LOCUS       DATLGF010000001         3611 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_158335, whole genome shotgun sequence.
ACCESSION   DATLGF010000001 DATLGF010000000
VERSION     DATLGF010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158335"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VM406_00005"
     CDS             <1..514
                     /locus_tag="VM406_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HVL74367.1"
                     /translation="NEISWFDWELAESDTGRAMMEFTRNCIAIRQQRPTLRSAEFLTA
                     TPRTVEGVPDTSWFDETGTEMTPERWSFAEGRLLALRRLAHSERLSHENAVAASLLLL
                     NAYSEDREFVLPPPYMPWRVAIDAAEPSGAPTVESAAQRIRDNRITVAAHSAVLLVAD
                     DVILDTGRQP"
     gene            774..2564
                     /gene="treZ"
                     /locus_tag="VM406_00010"
     CDS             774..2564
                     /gene="treZ"
                     /locus_tag="VM406_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HVL74368.1"
                     /translation="MNASTFTPSFGAVPIQTGSQPRTRFRFWAPNIEQAALEIEGREP
                     LPMNRLPGGWFGLEADCGAGARYRYRVRDDLAVPDPASRRQADDVHDASVVCDPNAWQ
                     WKHPDWRGRPWHETVLYELHPGICGGFDGIQKRLPELAALGITAIELMPIADFPGPRN
                     WGYDGVLHYAPDTAYGTPEQLKAMIDAAHGLGIMVFLDVVYNHFGPDGNYIGAYASDF
                     FNPDVHTPWGQAIDFRKRPVRDFFIENALYWLTEFRFDGLRFDAVHAIEEKDFLVEMA
                     ERIRAHVGESRHVHLVLENDENLASLLERAPRRLYDAQWNDDMHHAFHVLLTGERDGY
                     YCGYTAEPAQKLARGLAEGFIYQGEPSPFADNRPRGEPSAHLPPASFVVFLQNHDQIG
                     NRALGERLTTLADPRALRAAMGMLLLAPHIPMLFMGEEFGAREPFLYFTSHLTKELAD
                     AVRNGRRQEFARFPAFSDPAVRERIPDPNAEKTYTDSVPNPRRDDASDWLAWTRRLLE
                     LRRAHLFPRLEGATALGASAIGPAAVLARWRMGDGAVLTLAANLADAPAAVQPLPAGA
                     LLFETNAAAQGVARGELPAHAFLALLEEKA"
     gene            2561..>3611
                     /locus_tag="VM406_00015"
     CDS             2561..>3611
                     /locus_tag="VM406_00015"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HVL74369.1"
                     /translation="MSDQHILDLARAAGLALDWTNAHGEPMKVQPHALARILDALGLP
                     CASEQQARASHARLAEMEGRGALAPMVTGIVGHPLALPAIDGLAGRPWRIEFEGGGHL
                     EGHFAHDSGGGLQLPALEDFGYHRLLAGDAETTLAIAPRRCFGVADTVPQEEAARTRL
                     WGLGVQLYSLRRKGDGGYGDYTALADVARRAARTGAGAVAISPVHAMFSADVHRFSPY
                     GPSSRLFFNVLHIDPAAVLGPHALESALDELGNEAREELARLEQLELIDWPAASALRL
                     RLLRVLYKRFVARGDRAEFERYLAEAGEALQSHARFEALHAHRLATGQDAYWRHWPDA
                     LRDPRSAAVQAFARVR"
BASE COUNT          566 a         1218 c         1252 g          575 t
ORIGIN      
        1 caacgagatt tcctggttcg actgggagct ggccgagagc gacaccgggc gcgcgatgat
       61 ggagttcacc cgcaactgca tcgcgatccg ccagcagcgg ccgacactgc gcagcgccga
      121 gttcctcacc gcgacgccgc gcaccgtgga aggggtgccc gacaccagct ggttcgacga
      181 aacgggaacc gagatgacgc ctgagcgctg gagcttcgcc gaagggcgct tgctggccct
      241 gcgccgcctt gcgcattcgg agcgcctgag ccacgagaac gcggttgcgg cgtcgctgct
      301 gctgctcaat gcgtattcgg aggaccgcga attcgtgctg ccgccgccgt acatgccatg
      361 gcgggtggca atcgacgcgg ccgaaccgag cggcgcgccg acggtggagt cggccgcgca
      421 gcgcatccgc gacaaccgga tcacggtggc ggcgcacagc gcggtgctgc tggtggccga
      481 tgacgtgatt ctggatacgg ggaggcagcc gtgaggatgt tttgcgtgct tggcgctgct
      541 gcctgtcgcg ctgcctggtc cgggcaagag ctgtcgcgat cgccccatcc ctgtcgtccc
      601 cgcgcaggcg gggacccagt tccgccgcat caagtcgcgt cagcgcatcc cctgggatcg
      661 cgcctgcgcg ggagcgacat agcgagggaa aacgcttgaa tgaatagcat cccggccacg
      721 gccacgccgc cgccgcgacc atgcttccaa tcgaaaagac aagtcaggtt ttcatgaacg
      781 cctccacatt caccccatcc ttcggcgccg tcccgatcca aaccggctca caaccccgca
      841 cccggtttcg cttctgggcg ccgaacatcg aacaggcggc gctcgagatc gaaggccgcg
      901 aaccgctccc gatgaaccgg ctgccgggcg gctggtttgg gctggaagcc gattgcggag
      961 ccggtgcgcg ctaccgctac cgggtgcgcg acgatctcgc ggtgcccgac cctgcctcgc
     1021 gccgccaggc ggacgacgtg catgacgcat cggtcgtctg cgatccgaac gcctggcagt
     1081 ggaagcaccc ggactggcgc gggcggccgt ggcacgagac cgtgctgtac gagctgcacc
     1141 cgggcatctg cggtggcttc gacggcatcc agaagcgtct gccggaactc gcggcgctgg
     1201 gcatcaccgc gatcgagctg atgccgatcg ccgattttcc cggcccgcgc aactggggct
     1261 atgacggcgt gcttcactac gcgccggaca ccgcgtatgg cacgccggaa cagctcaagg
     1321 cgatgatcga tgccgcgcac ggactcggga tcatggtttt cctcgacgtg gtctacaacc
     1381 acttcggccc ggacggcaac tatatcggcg cctatgcgtc ggacttcttc aacccggacg
     1441 tgcacacgcc atggggccag gcgatcgatt ttcgcaagcg gccggtgcgc gacttcttca
     1501 tcgagaacgc gctgtactgg ctcaccgaat tccgcttcga cggcctgcgc ttcgacgccg
     1561 tgcatgcgat cgaggaaaag gacttcctgg tcgagatggc cgagcgcatc cgcgcccatg
     1621 tcggcgaatc ccggcatgtg catctcgtgc tggagaacga cgagaacctc gcgagcctgc
     1681 tggaacgcgc gccgcgccgc ctctatgacg cgcagtggaa cgacgacatg caccacgcgt
     1741 tccatgtgct gctgaccggc gagcgcgacg gttattactg cggctacact gccgaaccgg
     1801 cgcaaaagct cgcgcgcggc ctcgccgaag gcttcatcta ccagggcgaa ccgtcgccgt
     1861 tcgcggacaa ccggccgcgc ggcgagccca gcgcgcacct gccgccggca tcgttcgtgg
     1921 tgttcctgca gaaccacgac cagatcggca acagggcgct cggcgagcgg ctcaccacgc
     1981 ttgccgatcc gcgcgcgctg cgcgcggcca tgggcatgct gctgctggcg ccccatattc
     2041 caatgctgtt catgggcgag gaattcggcg cgcgcgaacc ctttctgtac ttcaccagcc
     2101 acctgacgaa ggagctggcc gatgcggtgc gcaacggcag gcgccaggag ttcgcgcgct
     2161 ttccggcgtt ctccgatccc gccgtgcgcg agcgcattcc ggacccgaac gccgagaaga
     2221 cctatacgga ctcggttccg aatccgcgac gggacgatgc aagcgactgg ctcgcatgga
     2281 cccggcgcct gctggagctg cgccgcgcgc acctgtttcc acgcctggaa ggcgcaacgg
     2341 cactcggggc aagcgcgatc gggccggccg cggtgctcgc gcgctggcgc atgggcgatg
     2401 gtgccgtgct gacgctggcg gcgaatcttg cggacgcgcc ggctgcggtg cagcccctgc
     2461 ccgcaggggc gctgctgttt gaaacgaatg ctgcagccca gggcgtggcg cgcggcgagc
     2521 tgccggcgca tgcgttcctg gcgctgctcg aggagaaggc atgagcgatc agcatattct
     2581 tgacctggcg cgtgcggccg gcctggcgct ggactggacc aatgcgcacg gtgagccgat
     2641 gaaggtacag ccgcatgcgc ttgcgcgcat tctggatgcg ctcgggctgc cgtgcgcgtc
     2701 cgagcagcag gcgcgcgcca gccatgcacg gctggcggaa atggaagggc ggggcgctct
     2761 ggcgccgatg gtcacaggca tcgtcggcca tccgctcgcg ctgcccgcga tagacgggct
     2821 tgccggccgg ccctggcgca tcgaattcga gggcggtggc cacctcgaag ggcactttgc
     2881 gcatgacagc ggcggcggat tgcagctgcc ggcgctggaa gatttcggct atcaccggct
     2941 gctggcgggg gacgcggaaa ccacgcttgc gatcgcgccg cgccgctgct tcggcgtggc
     3001 ggatacggtg ccgcaggaag aagcggcgcg tacgcggctg tgggggctgg gcgtgcaact
     3061 gtattcgcta aggcgcaagg gtgatggcgg ctatggcgac tacaccgcgc tggccgatgt
     3121 cgcgcggcgc gcggcgcgca ccggtgccgg ggcggtggcg atcagccccg tgcacgcgat
     3181 gttctccgcc gacgtgcatc gcttcagccc gtacgggccg tccagccgcc tgttcttcaa
     3241 cgtgcttcat atcgatccgg ccgccgtgct cggtccgcac gcactcgaat cggcgctgga
     3301 tgaactcggc aacgaggcgc gcgaggaact ggcgcgcctg gagcagctgg agctgatcga
     3361 ctggccggct gcatcggcgc tgcggctgcg cctgctgcgc gtgctgtaca agcgctttgt
     3421 cgcgcgcggc gatcgcgccg agttcgaacg ctatctcgcc gaggcaggcg aggcgttgca
     3481 gtcgcatgca cgcttcgagg cgctgcatgc gcaccgcctc gccaccgggc aggatgctta
     3541 ctggcggcat tggcccgatg cgctgcgcga cccgcgcagc gccgcggtgc aggcctttgc
     3601 ccgcgttcgg c
//
LOCUS       DATLGF010000002         5192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_31809,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000002 DATLGF010000000
VERSION     DATLGF010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31809"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VM406_00020"
     CDS             complement(<1..495)
                     /locus_tag="VM406_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HVL74370.1"
                     /translation="MDDIVRQAMAKWPNVPHCYGWLALDARGTWRMRDDRAQSMNLPG
                     DPIRNAALVGFINRNYLHGDQGRWYFQNGPQRVYVNLETTPYIARTDPAHGFLLHTGD
                     ALGPVEAAWLTDDGRLVLKNGERIAQVDDRDIAQCLPALCIDGKSVSDEALMAWLEQP
                     EYGRL"
     gene            complement(497..1438)
                     /locus_tag="VM406_00025"
     CDS             complement(497..1438)
                     /locus_tag="VM406_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVL74371.1"
                     /translation="MLYTSPSWLFGGHLQTIVPATLMRRPPVQYRRERWTAPDGDFVD
                     VDFIDGEPDRPFLVLFHGLEGSSASHYARALMAEIAALGWSGAVPHFRGCSGELNHAP
                     RFYHSGDAAEIEWIARRLAERCTGRRFYTVGVSLGGNAMLRWLGESQHQADFVDAACA
                     ISAPLDLAAGGAALSRGVNMIYTRHFLQTLKPKCLQKLAQHPGLFDRDAMLGARNLYE
                     FDNVVTAPLHGYRDTDDYWNRASARHVLPDITVRTLVLNAQNDPFLPGHHLPRHASSA
                     VTLDYPLHGGHVGFAVGRLPGRINWLPQRILHFLQEA"
     gene            complement(1449..1880)
                     /locus_tag="VM406_00030"
     CDS             complement(1449..1880)
                     /locus_tag="VM406_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVL74372.1"
                     /translation="MSTTQFHPETVAEIEAAFYAAVARADVEAVMALWANDETVVCIH
                     PGAPRLVGHASIRASWESIFEQGGVQIRPVQLHATRNAETAVHSIIEEVHRSGTQRPD
                     IHILATNVYMKTARGWRMVAHHASVVPGEAPPDEPAASMLH"
     gene            complement(2046..2246)
                     /locus_tag="VM406_00035"
     CDS             complement(2046..2246)
                     /locus_tag="VM406_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HVL74373.1"
                     /translation="MTQAKQDMSYVELDADDLPAYCPNPSMPIWSSHPRVFIPLDVNH
                     TGQCPYCGTVYKLKPGAVVKGH"
     gene            complement(2265..3185)
                     /locus_tag="VM406_00040"
     CDS             complement(2265..3185)
                     /locus_tag="VM406_00040"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HVL74374.1"
                     /translation="MSMADRDGKIWKDGELIEWRDATVHVLTHTLHYGMGVFEGVRAY
                     KTDKGTAIFRLKEHTQRLFNSAKIFQMQIPYDFETILEAQRQVVRENKLESCYLRPLV
                     WVGSEKLGIAAKGNTIHVAIAAWPWGAYLGEDGLAKGIRVKTSSFTRHHVNVSLVRAK
                     ACGYYINSILANQEALADGYDEALLLDTEGYVSEGSGENVFIVKNGKIYTPDLASCLD
                     GITRDAVLTMARDLGIEVIEKRITRDEMYCCDEAFFTGTAAEITPIRELDNRQIGEGR
                     RGPITEKLQSLFFDVVAGKAPQYAHWLTLV"
     gene            3414..4163
                     /locus_tag="VM406_00045"
     CDS             3414..4163
                     /locus_tag="VM406_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HVL74375.1"
                     /translation="MRNGEPGVAPPPAIDLERRAFQEGVRGAAPTMLGICAWGMVTGM
                     AMVKTGLTIPQALGMSFLVFAGSAQLAALPLIAANVPVWVVFVTAMIVNLRFVIFSAA
                     VGPHFAHLPWYRRLWHGYFNADIMMALFPRRFPPSTMDKPVGKVGFYNGIAYSNWVAW
                     QVGSVAGILLASQIPAHWGIGFAGTLALLAITIPLTVNLAALVGVITASIVAVAAAAL
                     PYRLGLLLGVIIGMVAAMIADTLLDKKKEHA"
     gene            4160..4483
                     /locus_tag="VM406_00050"
     CDS             4160..4483
                     /locus_tag="VM406_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HVL74376.1"
                     /translation="MSALDVWLTILLLTLATLLTRTPFFLLGNAVKIPPKVQHALRYA
                     PAAALAAIVAPDLVMTEGALHLDWTNPRLMAGIGATAFFLATRHLLGTIIVGMALFTL
                     LRVIL"
     gene            4560..>5192
                     /gene="pgk"
                     /locus_tag="VM406_00055"
     CDS             4560..>5192
                     /gene="pgk"
                     /locus_tag="VM406_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HVL74377.1"
                     /translation="MQFKRLTDLASNNQLQGKRVFIRADLNVPQDDAGNITEDTRIRA
                     SVPAIRQALDAGAAVMVTSHLGRPTEGEFKPEDTLAPIAERLSELLGQPVELKQNWVD
                     GVEVAPGKVVLLENCRVNKGEKKNSDELAQKMAKLCDVYVNDAFGTAHRAEATTHGIA
                     KYAPIACAGPLLAAELDALGKALGQPARPLVAIVAGSKVSTKLTILKSLAA"
BASE COUNT          842 a         1623 c         1722 g         1005 t
ORIGIN      
        1 cagccggccg tattccggct gctcgagcca cgccatcagc gcttcatcgc tgaccgattt
       61 cccatcgatg cataacgcgg gcaggcactg cgctatgtcg cggtcgtcga cctgcgcaat
      121 cctttcaccg tttttcagga cgagccgccc atcgtcggtg agccatgcgg cttccacggg
      181 gccgagtgcg tcgccggtgt gcagcaggaa tccgtgagcc gggtccgtgc gggcgatgta
      241 cggcgtcgtt tccaggttca cgtagacccg ctgcgggccg ttctggaagt accagcgtcc
      301 ttggtcgccg tgcaggtagt tgcggttgat gaagccgacc agcgcagcat tgcggatcgg
      361 atcgccgggc aggttcatcg actgggcgcg atcgtcgcgc atgcgccaag tgccgcgcgc
      421 atcgagcgcc agccagccgt agcagtgcgg cacgttcggc cacttggcca tcgcttgtct
      481 gacgatgtcg tccatcctag gcctcctgca ggaagtggag tatgcgttgc ggcagccagt
      541 tgatgcgtcc gggcaggcgt cccaccgcaa agccgacgtg cccgccgtgc agcggatagt
      601 cgagcgtcac ggcggatgaa gcgtggcgcg gcaggtggtg gcccggcagg aaggggtcgt
      661 tctgcgcgtt gagcaccagt gtgcgcacgg tgatgtcggg cagcacgtga cgggcgctgg
      721 cgcggttcca gtagtcgtcg gtatcgcgat agccgtgcag cggcgcggtg acgacgttgt
      781 cgaactcgta caggttgcgc gcaccaagca tggcgtcgcg atcgaacagg cccgggtgct
      841 gcgcaagctt ctgcaggcac ttcggcttga gcgtctgcag gaaatggcgg gtgtagatca
      901 tgttcacccc gcgcgagagc gccgcgcctc cggccgccag gtcgaggggc gccgagatcg
      961 cgcaggccgc atcgacgaag tcggcctggt gctgggactc gccgagccag cgcagcatcg
     1021 cgttgccccc gagcgagacg ccgacggtgt agaacctgcg gccggtgcaa cgttcggcca
     1081 ggcggcgcgc gatccactcg atctcggcgg cgtcgccgga gtgatagaag cgcggtgcat
     1141 ggttcagctc gcccgaacag ccgcgaaagt gcggcaccgc accggaccag ccgagcgccg
     1201 ctatctcggc catcagtgcg cgcgcatagt ggctggcgga cgagccttcg agaccatgga
     1261 acagaaccag aaatggccgg tctggctcgc catcgatgaa gtccacatcg acgaaatcgc
     1321 catccggcgc agtccagcgc tcacgccggt actggacggg cgggcggcgc atcagcgtcg
     1381 cgggcacgat ggtctgcaga tgaccgccga acagccatga gggcgaggtg tacagcatgg
     1441 cggtcaggct agtgcagcat ggacgccgcc ggttcgtccg gcggcgcttc gccgggcacg
     1501 accgaggcat ggtgcgcgac catgcgccag ccgcgcgcgg ttttcatgta gacgttggtg
     1561 gcgaggatat ggatgtcggg gcgctgcgtg ccgcttctgt gcacttcctc gatgatgctg
     1621 tgcaccgcgg tctccgcatt gcgggtcgca tgcagctgca cggggcggat ctgcacgccg
     1681 ccttgctcga agatcgactc ccacgacgcg cgtatcgacg catggccgac caggcgcggc
     1741 gcgcccggat ggatgcagac gacggtctcg tcattggccc acagcgccat gacggcttcg
     1801 acgtcggcgc gggcgacggc ggcatagaac gcggcttcga tctcggccac ggtttccggg
     1861 tggaattggg tagtggacat gtcggctgac aggcgatgct tgtggctcag tcgactattg
     1921 tacgagtgat tcgttcgatg ccgcagaaga ggcgggcttg gatgccggag ggtaagtcgg
     1981 gtggcttgga cgccggttgc ttccggcgtc caagccgtcg ttcaaacgag cattgcactg
     2041 tgcgttcaat gccccttgac gacggcgccc ggcttgagct tgtagacggt accgcaatac
     2101 gggcactgcc cggtgtggtt gacgtccagc gggatgaaca cgcgcggatg cgaggaccag
     2161 atcggcatgc tcggattcgg gcagtaggcc gggaggtcgt ccgcgtcgag ctccacgtag
     2221 ctcatgtctt gttttgcttg ggtcatggcg gtgcagggaa gtgattaaac cagggtcagc
     2281 cagtgcgcgt actgcggcgc cttgccggcc accacatcga agaacagcga ctgcagcttc
     2341 tccgtgatcg ggccgcgcct gccttcgccg atctggcggt tgtcgagctc gcgtatcggc
     2401 gtgatctcgg cggcggtgcc ggtgaagaag gcttcgtcgc agcagtacat ctcgtcgcgc
     2461 gtgatgcgct tctcgataac ctcgatgccg aggtcgcgcg ccatcgtcag caccgcgtcg
     2521 cgggtgatgc cgtccaggca ggaggcaaga tcgggcgtgt agatcttgcc gttcttgacg
     2581 atgaacacgt tctcgccgga cccttccgac acataacctt cggtgtcgag cagaagcgct
     2641 tcgtcgtagc cgtcggccag cgcttcctgg ttcgcgagaa tggagttgat gtagtagccg
     2701 caggccttcg ctcgcaccag cgacacgttc acatgatggc gagtgaacga cgaggtcttc
     2761 acgcggatgc ctttggcgag gccgtcttcg ccgaggtagg cgccccaggg ccaggcggcg
     2821 atcgcgacgt ggatggtgtt gcccttggcc gcgatgccga gcttctcgga gccgacccat
     2881 acgagcggac gcagatagca ggattcgagc ttgttctcgc gaacgacctg gcgctgcgct
     2941 tcgaggatgg tttcgaagtc gtaggggatc tgcatctgga agatcttggc cgagttgaac
     3001 aggcgctgcg tgtgctcctt caaccggaag atggccgtgc ctttgtcggt cttgtaggcg
     3061 cgcacgccct cgaacacgcc catgccatag tgcagggtgt gggtaagcac gtgtaccgtg
     3121 gcatcgcgcc attcgatcag ttcgccgtct ttccagattt ttccgtcgcg gtctgccatg
     3181 gacatgtgtg tgctccagtg tcgttcagaa ggcaagattt tacccggaac acgcgttgtc
     3241 cggttcgtcg ctagaatagc tggtttcgcc cttttccatt cccatccttc ctccatggca
     3301 gtgcaccttt tccccgcctg gtcggcttcc ccggcaatgc gtcgtgccac gccttttctt
     3361 cagtcttttt ccggccgcgc ttggaaagtc gcggccagtt ctggttatct gctatgagaa
     3421 acggcgaacc cggcgtcgcc cctccgcccg cgatcgacct cgaacgccgc gcgtttcaag
     3481 aaggcgtgcg cggcgcggcg ccgaccatgc tgggcatctg cgcctggggc atggtgacgg
     3541 gcatggcgat ggtcaagacc ggactgacca tcccgcaggc gcttggcatg agctttctcg
     3601 tgttcgccgg ctcggcccaa ctggcggcac tgccgctgat cgcggcgaac gtgccggtat
     3661 gggttgtgtt tgtgacggcc atgatcgtca acctgcgctt cgtgattttt tccgcggcgg
     3721 tcgggcctca cttcgcgcat ctgccgtggt accggcgcct ctggcacggt tacttcaatg
     3781 cagacatcat gatggcattg ttcccgcgcc gtttcccgcc ttcgacgatg gataagccgg
     3841 tcgggaaggt tggcttctac aacgggatcg cctactcgaa ctgggttgca tggcaggtcg
     3901 gctcggtcgc cgggattctg cttgccagtc agattcccgc acactggggg atcggttttg
     3961 ccggtacgct cgccctcctt gcgattacca taccgctcac cgtcaatctc gccgcgctgg
     4021 ttggcgtgat cacggccagc atcgtcgcgg tggctgccgc ggccctgccg tatcggctgg
     4081 gtctgctact cggggtcatt atcgggatgg ttgcggcgat gattgccgat acgctgcttg
     4141 acaagaagaa ggaacatgca tgagcgcact cgatgtctgg ctgaccatcc tgctgctgac
     4201 gctggcaacc ctgctcaccc ggaccccgtt cttcctgctc gggaatgcgg tcaagatccc
     4261 tccgaaagtc cagcacgcgc tgcgctacgc gccggcggcg gcgttggcgg cgatcgtcgc
     4321 tcccgacctg gtaatgaccg aaggagcgct gcacctggac tggacgaacc cgaggctgat
     4381 ggccggcatt ggcgccacgg ccttcttcct cgcgacgcgc catttgctgg gcacgatcat
     4441 cgtcggtatg gccttgttca ctttgctgcg cgtcatcttg tagggccgta acgctttttt
     4501 ggcaacccgg ctgcggtaaa ataccggttt ttccattcct tcatcccgcc tccgcagcca
     4561 tgcaattcaa gcgcctgact gatctcgcct ccaacaacca gctccaggga aaacgcgttt
     4621 tcatccgtgc cgacctcaac gtcccgcagg acgacgccgg caacatcacc gaggacaccc
     4681 gcatccgcgc atccgttcct gcgatccgcc aggcgctcga cgccggcgcc gcggtgatgg
     4741 tgacctcgca cctgggccgg ccgaccgaag gcgagttcaa gccggaagac acgctcgcgc
     4801 cgatcgccga gcggctgtcg gaactgctgg gccagccggt cgagctgaag cagaactggg
     4861 ttgacggcgt cgaggtggcg cccggcaagg tggtgctgct cgaaaactgc cgcgtgaaca
     4921 agggcgagaa gaagaacagc gacgaactcg cgcagaagat ggccaagctg tgcgacgtct
     4981 acgtgaacga tgcgttcggc accgcgcacc gcgcggaagc caccacccat ggcattgcga
     5041 agtacgcgcc gatcgcctgc gccggcccgc tgctggctgc cgagctcgat gcgctgggca
     5101 aggcgctcgg ccagccggcg cgtccgctcg tggccatcgt cgccggctcc aaggtgtcga
     5161 ccaagctgac catcctgaaa tcgctcgccg cg
//
LOCUS       DATLGF010000003         2982 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_221775, whole genome shotgun sequence.
ACCESSION   DATLGF010000003 DATLGF010000000
VERSION     DATLGF010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221775"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1433
                     /locus_tag="VM406_00060"
     CDS             <1..1433
                     /locus_tag="VM406_00060"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HVL74378.1"
                     /translation="AKSSQQLAELAAELGALGGVHVVVGHPLAEDGKRYNAASVLADG
                     KVIGTYRKHDLPNDTVFDEERYFDSVDQPFVFEVKGVRFGINLCEDTWSPHAPARAKE
                     AGAQVLLIPNGSPYHMHKQHRRYEVMRANVSVLGMPLVYANLVGGQDELVFDGNSFVM
                     DGMGVVCAQLRHCEEDLQVIEFDGAQPGTGTIAPELPIEAVVYQALVMGVRDYLGKNG
                     FPGAIIGLSGGVDSALTLAIAVDALGAERVRAVMMPSPYTAEISWVDSREMAARIGVR
                     YDEIPISRCFDAFRGTLAEEFKGLAEDTTEENIQARIRGTILMALSNKYGSIVLTTGN
                     KSEMAVGYCTLYGDMAGGFAVIKDIAKTFVYRLCRYRNSLSEVIPERILTRAPSAELR
                     PDQTDQDSLPEYEVLDGIMQMYMEEDRSIAEILAAGYRKEDVERVTRLIKVNEYKRRQ
                     APIGVRITPRAFGRDWRYPITSKFRD"
     gene            1455..1793
                     /locus_tag="VM406_00065"
     CDS             1455..1793
                     /locus_tag="VM406_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HVL74379.1"
                     /translation="MKLITAIIKPFKLDEVRESLGEVGVTGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEAVVDESVVDQAVDAIIKAARTGKIGDGKIFVRNVEQVIRI
                     RTGETGRDAV"
     gene            complement(1850..2452)
                     /locus_tag="VM406_00070"
     CDS             complement(1850..2452)
                     /locus_tag="VM406_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HVL74380.1"
                     /translation="MTLIKIIEVMAILVGAFSGFVEARSKKMDVVGVFTVAFITAFGG
                     GTLRDILLDKRPLFWVTHQEYAILIFVLALIATPLIRTLRKIVSERLIVIADALGLGL
                     FSIAGVAQALAAGMPLFIASMMGVITGIFGGVLRDIVCNEIPMVLRDGRPYAICSFAG
                     CWAYIVMHEAAVAPTFALWSSALLICGLRLASWTLDVKVK"
     gene            complement(2698..>2982)
                     /locus_tag="VM406_00075"
     CDS             complement(2698..>2982)
                     /locus_tag="VM406_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HVL74381.1"
                     /translation="LRRGHWVIQGQLDLHGMRRDEAREALGEFLRNAVKRGIRCVRIV
                     HGKGLGSVNKEPVLKNKVRSWLVQKDEVMAFCQARGDEGGAGAVVVLLRA"
BASE COUNT          558 a          938 c          969 g          517 t
ORIGIN      
        1 acgcaaaatc gtcccagcag ttggccgagc tcgccgccga actcggcgcc cttggcggcg
       61 tgcacgtggt ggtcggtcac ccgttggccg aggacggcaa gcgctacaac gcggcgtccg
      121 tgctggccga tggaaaggtg atcggcacct atcgcaagca cgatttgccg aacgacaccg
      181 tgttcgacga ggaacgctat ttcgattcgg tcgaccagcc cttcgtgttc gaggtcaagg
      241 gtgtgcgttt cggcatcaac ttgtgcgagg atacctggtc gccgcacgcc ccggcgcgcg
      301 cgaaggaggc aggcgcccag gtgctgctga ttccgaacgg ctcgccctac cacatgcaca
      361 agcagcatcg gcgctacgag gtgatgcgcg ccaatgtgag cgtgctcggg atgccgcttg
      421 tgtatgcgaa tctcgtcggc ggccaggatg aactggtgtt cgacggcaat tccttcgtga
      481 tggatggcat gggtgtcgtg tgcgcccagc tcaggcactg cgaggaagac ctgcaggtga
      541 tcgagttcga cggggcgcag cccggaactg gcacgatcgc accggagctg ccaatcgagg
      601 cggtggtgta ccaggcgctg gtcatggggg tgcgcgacta tctcggcaag aacggctttc
      661 ccggcgcgat catcggcttg tcgggcggcg tcgattcggc gcttacgctg gcgatcgcag
      721 ttgacgcgct cggcgcggaa cgcgtgcggg cggtgatgat gccctcgccg tatacggccg
      781 agatctcgtg ggtcgactcg cgcgagatgg ccgcgcgcat cggcgtgcgc tacgacgaga
      841 ttccgatttc ccggtgcttc gatgccttcc gcggcacgct ggcggaggaa ttcaaggggc
      901 tggccgagga tacgaccgaa gagaacatcc aggcgcgcat ccgcggcacc atcctcatgg
      961 cgctgtcgaa caagtacggc tccatcgtac tcaccaccgg caacaagagc gaaatggccg
     1021 tcggctactg cacgctgtac ggggacatgg ccggcgggtt tgcggtcatc aaggacatcg
     1081 ccaagacctt cgtctaccgg ctgtgccgtt atcgcaacag cctgtccgag gtaatcccgg
     1141 agcgcatact cacgcgcgcg ccgtccgccg agctgaggcc tgaccagacc gaccaggatt
     1201 cgctgcccga gtacgaggtg ctcgacggga tcatgcagat gtacatggaa gaggaccgca
     1261 gcatcgccga gatcctcgcc gccggctacc gcaaggagga tgtcgagcgg gtcacgcgcc
     1321 tgatcaaggt gaatgaatac aagcggcgcc aggcgccgat cggcgtgcgc atcacgccgc
     1381 gtgcgttcgg gcgtgactgg cgctatccga tcacttcgaa attccgtgat taacttttca
     1441 agcaggggga agcgatgaaa ctgattaccg ccatcatcaa gccgttcaag ctcgacgaag
     1501 tgcgcgaatc cttgggcgaa gtaggggtga ccggcctgac cgtcaccgaa gtcaagggct
     1561 tcggacgcca gaaggggcac acggagctgt accgcggcgc cgagtacgtg gtcgatttcc
     1621 tgccgaaggt gaagatcgag gcggtggtcg acgagagcgt ggtcgaccag gcggtcgacg
     1681 cgatcatcaa ggccgcgcgc accggcaaga tcggcgacgg caagatcttc gtgcgcaatg
     1741 tcgagcaggt catccgcatc cgcaccggcg aaaccggacg ggacgcggtt taatatccgt
     1801 cctgccggcg tgggcacggc gcgttcaggc gctgccgtgc ataccgaact tatttcacct
     1861 tcacgtccag cgtccagctc gccagccgca agccgcagat cagcagcgcg ctcgaccata
     1921 gcgcgaacgt cggtgccacc gcggcttcgt gcatcacgat gtaggcccag cagccggcga
     1981 acgagcagat cgcatacggg cgtccatcac gcagcaccat cgggatctcg ttgcaaacga
     2041 tgtcgcgcag cacgccgccg aagatcccgg tgatcacccc catcatcgac gcgatgaaga
     2101 gcggcatgcc ggcagccagc gcttgcgcta cgcctgcgat cgagaacagc cccagtccca
     2161 gcgcgtcggc gatcacgatc aaacgctccg agacgatctt gcgcaaggtg cggatcagcg
     2221 gtgtggcaat caaggccagc acgaagatca ggatcgcgta ctcctggtgc gtgacccaga
     2281 acagcgggcg cttgtcgagc aggatgtcgc gcagcgtccc gccgccgaac gcagtgatga
     2341 acgcgaccgt gaatacgcct accacgtcca tcttcttgct gcgcgcctcg acgaaacctg
     2401 agaacgcgcc gaccaggatg gccatgactt cgatgatttt gatgagggtc atgcgagcgg
     2461 ctcgagtgga gtcactgcat catacctttg gagctcgttt gggttgtgcc gcggcgtgca
     2521 tggaggctgg cgaggccttg tcattccgag tgcagcgatg aacctccggt gcatgggtcg
     2581 acgggattcc tcgctgcgct cgggacggcc ggtgatcgtg gtcaatcgag gtttgcgcca
     2641 tgcccgtccg ccgtgtgcgc gatgcgcgcc gtgaatcaag aaaacattcg gtcccgctca
     2701 tgccctcagc agcaccacca cagcgccggc cccgccttcg tcgccgcgcg cctggcagaa
     2761 ggccatcact tcatccttct gcaccagcca gctgcgcacc ttgttcttca gcaccggttc
     2821 cttgttcacc gagcctagcc ccttgccgtg gacgatgcgc acgcagcgga tgccgcgctt
     2881 gacggcgttg cgcaggaatt cgccgagtgc ttcacgcgcc tcgtcgcggc gcatcccatg
     2941 caggtcgagc tggccctgga tcacccagtg gccgcgccgc ag
//
LOCUS       DATLGF010000004         3533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_152,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000004 DATLGF010000000
VERSION     DATLGF010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /gene="rlmN"
                     /locus_tag="VM406_00080"
     CDS             <1..970
                     /gene="rlmN"
                     /locus_tag="VM406_00080"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HVL74382.1"
                     /translation="ISDHTSADGTRKWLLDVGQGNAVETVFIPEENRGTLCVSTQAGC
                     AVNCRFCSTGKQGFSRNLTVAEIVGQLWMAEFALRRSKGIEPGQGGKKSGERQITNVV
                     MMGMGEPLLNFEPTVTALKLMLDDNAYGLSRRRVTLSTSGVVPMIDRLATECPVALAV
                     SLHASNDALRDGLVPLNRKYPLAELMAACRRYLEFAPRDFITFEYCMLDGVNDADENA
                     RELVELVRRHNVPCKFNLIPFNPFPESGLVRSNNVRIKAFAQVLIDAGIVTTVRKTRG
                     DDIDAACGQLAGEVQDRTRVQERMQKMAEYQNKYGKNFGRIVEVPR"
     gene            967..1764
                     /gene="pilW"
                     /locus_tag="VM406_00085"
     CDS             967..1764
                     /gene="pilW"
                     /locus_tag="VM406_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HVL74383.1"
                     /translation="MSFARCFSTLAASAALMLLAGCAATPDPTDTFVQAGGSEQTENE
                     RRARIRLQLAVGYYEQRQFAVALEELRLALQADPRLADGYSMRGLIFMEMGENKLAED
                     SFQHALRLAPNAPDYNNNYGWFLCQTGRERQSIAYFENALRSRNYQSPAVALANAGTC
                     SLRVNELAAAERYLLQSFEIDAANPTTAFNLARLYHARRDDERARFYIARVMKADLMT
                     AEVLWLALRIERRLGDRAAEASLANQLRRRFPASPEFAAFQRGAFDE"
     gene            1757..2698
                     /locus_tag="VM406_00090"
     CDS             1757..2698
                     /locus_tag="VM406_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HVL74384.1"
                     /translation="MSEPENNLNPPAQEQDNPPSPAPDAPLSPGAKLAAGRQERGWTV
                     EQVAAHLKLAPRQVLAIESDDYAALPGMPIVRGFIRSYAKLLRIDAAPLLEQVGGAPA
                     SGQESLKPQQDLATPFAGGRVPSMSEKPGLSFKWIVGVLLLVLVAAAIWALQNGARDV
                     DSPPQAVTAEQEPAGVVEEPLPEVASDGDTPPGADPVQPEPPAATPAAPGAVPPPVTA
                     APARDAAAATQTPAAGPNQLTLRVREESWIEVRRAGGGNPLFARLARAGEDISVEVNE
                     PVVLVVGNAAGVDVALRGEPVALRASTTSNVARVTLK"
     gene            2707..>3533
                     /locus_tag="VM406_00095"
     CDS             2707..>3533
                     /locus_tag="VM406_00095"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HVL74385.1"
                     /translation="MSDCPISSGPAPRRFARGVTIRHGDRAVQMGGHAPVVVQSMTNT
                     DTADAIATAIQVKELARAGSELVRITVNTPEAAAAVPAIREQLDRMGIDVPLIGDFHY
                     NGHKLLQDYPECAQALSKYRINPGNVGQGAKRDTQFAQMIEVACRYDKPVRIGVNWGS
                     LDQSLLARIMDENAKRAQPWSAQAVMYEALVTSAIENAQRAEELGLAADRIVLSCKVS
                     GVQDLIAVYRELARRCDYPLHLGLTEAGMGSKGIVASTAALAVLLQEGIGDTIRVSLT
                     "
BASE COUNT          624 a         1154 c         1170 g          585 t
ORIGIN      
        1 catcagcgac cacacctccg ccgacggcac ccgcaagtgg ctgctcgacg tcggccaggg
       61 caatgcggtc gagaccgtat tcattcccga agagaaccgt ggcacgctgt gcgtgtcgac
      121 ccaggccggc tgcgcggtca actgccgctt ctgctcgacc gggaagcagg gcttctcgcg
      181 caacctcacc gtcgccgaga tcgtcggcca gttgtggatg gccgaattcg cgctgcgcag
      241 gagcaagggc atcgagcccg ggcaaggtgg gaagaaaagt ggcgagcgcc agatcaccaa
      301 tgtcgtgatg atgggcatgg gcgagccgtt gctcaatttc gagccgacgg ttaccgcgct
      361 caagctgatg ctcgacgata acgcctacgg cctgtcgcgc cgccgggtga cgctgtcaac
      421 ctccggcgtg gtgccgatga tcgaccgtct cgccaccgag tgtccggtcg cgctggccgt
      481 gtcgctgcat gcgtccaacg acgcgctgcg cgacgggctg gtgccgctca accgcaagta
      541 tccgctggcc gagctgatgg ccgcctgccg ccgctatctc gaattcgcgc cacgcgactt
      601 catcaccttc gaatactgca tgttggacgg cgtcaatgac gccgacgaga atgcgcgcga
      661 actcgtcgag ctggtgcgcc gccacaatgt cccgtgcaag ttcaacctga tcccgttcaa
      721 tccctttcct gagtccggcc tggtgcgctc caacaacgtg cgcatcaagg cattcgccca
      781 ggtcctgatc gacgccggca tagtgacgac ggtgcgcaag acgcgcggcg acgacatcga
      841 cgcggcctgc ggacagctcg caggcgaggt gcaggatcgc acccgcgtgc aggaacgcat
      901 gcagaagatg gcggaatacc agaacaaata cggcaagaat ttcggacgca tagtggaggt
      961 tcccaggtga gctttgcacg ctgtttttcg acgctcgcgg cgagcgccgc gctgatgctg
     1021 ctcgccggtt gcgccgcgac gccggatccg accgatacct ttgtccaggc cggcggttcc
     1081 gagcagacgg agaatgaaag gcgcgcgcgc attcgcctgc agcttgcggt cggctattac
     1141 gagcagcgac agttcgccgt cgcgctggag gaattgcgcc tggcgcttca ggccgatccg
     1201 cgtctggccg acggttacag catgcgcggc ctgatcttca tggagatggg cgagaacaag
     1261 cttgccgaag acagcttcca gcatgctttg cgtctggcgc cgaacgctcc ggattacaac
     1321 aacaactacg gctggttcct gtgccagacc ggccgtgaac gccagtcgat cgcctatttc
     1381 gagaatgcgc tcagaagccg caactaccag tcgcccgcgg tcgcgctggc caatgcgggc
     1441 acctgcagcc tgagggtcaa cgaacttgcc gctgccgaac gctacctgct gcagtccttt
     1501 gagatcgacg cggccaatcc gaccaccgcc ttcaatctcg cccgcctcta tcacgcccga
     1561 cgcgatgatg agcgtgcccg cttctatatc gcccgtgtga tgaaggccga cctgatgacg
     1621 gcggaagtgc tgtggctggc gctgcgtatt gagcggcggc tgggcgatcg cgctgccgag
     1681 gccagccttg ccaatcaatt gcgccgccgc ttcccggcgt cgccggagtt tgccgcattt
     1741 cagagaggcg cattcgatga gtgaaccgga aaacaatctg aatccgccgg ctcaagaaca
     1801 ggacaatccc ccgtcaccgg cgcccgatgc accgctttcc cccggcgcga agctggccgc
     1861 gggccggcag gagcgtggct ggacggtgga gcaggtcgcc gcgcacctga agctggcgcc
     1921 gcgccaggtt ctcgccattg aaagcgatga ctatgctgcg cttccgggca tgccgatcgt
     1981 gcgcggattc atccgctcct acgcaaagct gctgcggatc gatgctgcac ccttgctcga
     2041 gcaggtgggc ggcgcaccgg cctcgggcca ggaatcgctc aagccgcagc aggatctagc
     2101 aaccccgttt gcaggcgggc gggttccctc gatgagcgag aagccgggtc tgtcgttcaa
     2161 atggatcgtc ggtgtgctct tgctggtact cgtggccgcc gcgatctggg cgctgcagaa
     2221 cggagcccgg gacgtcgact cccctcccca ggccgtaacg gctgagcagg agccggccgg
     2281 cgtggtcgag gaaccgttgc cagaagtcgc atcggacggc gacaccccgc cgggagccga
     2341 tccggtgcag ccggagcccc ccgccgcgac gcctgcagcg ccgggagcgg tgccgccgcc
     2401 cgttactgcc gcccccgcgc gggatgctgc ggctgcgacg cagacgccgg cggccggacc
     2461 gaaccagctg acgctgcggg tgagggaaga atcatggatc gaagtgcgcc gggcaggcgg
     2521 aggcaacccg ctgttcgccc gtcttgcgcg cgcgggcgag gatatttcgg tggaagtgaa
     2581 tgagccggta gtgctggtcg ttggcaatgc cgccggcgtc gatgtcgcat tgcgcggcga
     2641 gccggttgcc ctgcgggcca gcacgaccag caatgtcgca cgcgtcacgt tgaagtgagt
     2701 ctggccatga gcgattgccc catttcttca ggtccggcgc cacgtcgctt cgcacgcggg
     2761 gtgacgatcc gtcatggcga tcgtgcggtc cagatgggcg gccacgcccc ggtcgtcgtg
     2821 cagtcgatga ccaataccga caccgccgac gcgatcgcca ccgcgatcca ggtgaaggag
     2881 ctcgcgcgcg cgggttcgga attggtgcgc atcacggtaa ataccccgga agccgccgcc
     2941 gccgtgcccg cgatccgcga gcagctcgac cgcatgggca tcgacgtgcc tctgattggc
     3001 gattttcact acaacggcca caagctgctg caggattatc cggaatgcgc ccaagccttg
     3061 tcgaagtacc gcatcaatcc gggcaacgtc gggcagggcg ccaagcgcga cacgcagttt
     3121 gcgcagatga tcgaggtggc ctgccgttac gacaagccgg tgcgcatcgg ggtgaactgg
     3181 ggcagcctgg accagtcgct gctggcgcgc atcatggacg agaacgccaa gcgcgcccaa
     3241 ccctggagcg cgcaggcggt gatgtacgaa gcattggtga cgtccgccat cgagaacgca
     3301 cagcgcgccg aagagctcgg cctggccgcc gacaggatcg tcctgtcgtg caaggtatcg
     3361 ggcgtgcagg acctgatcgc cgtctaccgc gaactggcgc gccgctgcga ctatccgctg
     3421 cacctgggac tgaccgaggc cggcatgggc agcaagggca tcgtcgcgtc gacggctgcg
     3481 ctcgccgtgc tgctgcagga aggcatcggc gacacgattc gcgtgtcgct gac
//
LOCUS       DATLGF010000005        14254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_47729,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000005 DATLGF010000000
VERSION     DATLGF010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 14254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14254
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47729"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="VM406_00100"
     CDS             <1..1098
                     /locus_tag="VM406_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVL74386.1"
                     /translation="TACRSSEGMTMNGMQWMFETMDAWRRRQGNAFDLAGLGPREVDC
                     QIDLDMPALRLRCYGGPEARAVLAVPAPIKRHYIWDLLPDCSAVRRLLDAGYRVYLIE
                     WKDPQGEMAEAGLQQYGSDWIDACVQRIRQDCPQTPLVLISHSLGGIFATIYASLHPE
                     QVRALVLLEVPLHFGKASGAFLPLLLAAPPTPLLLRDMTRVPGSLLSMVSAMASPRTF
                     HGERAADFAASLASPADLQRHMLVERWALDEAPMANLLFEQIVEDLYRNDKLMRGTLE
                     ISGTTIGPAQMRVPLFAVHDPASDIIPSESVTTYVDAAASDDKALATVESERGTGLSH
                     VAALVGPRAHALLWPRILSWLRNLPERRTGA"
     gene            complement(1154..1774)
                     /locus_tag="VM406_00105"
     CDS             complement(1154..1774)
                     /locus_tag="VM406_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74387.1"
                     /translation="MQKKSLAAFISALFVSSAAFATTGQPPQTEIRESTDPAVVAEVE
                     QRASEIIARQQDQQPGQASGETGVTGAGAAGEGAAGAARERDQERNGRSRDASGATGT
                     DATTGATGTDATTGATRTDATTGATGTDATTGATGTDATTGATGTDATTGATGTDATT
                     GATGTDGAARTGIDAPGATGTTDTTGTAGAGTSGGLHQDSPRQQQQ"
     gene            complement(1915..2427)
                     /gene="bamE"
                     /locus_tag="VM406_00110"
     CDS             complement(1915..2427)
                     /gene="bamE"
                     /locus_tag="VM406_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HVL74388.1"
                     /translation="MKSLYPAFAVLFFLMLVGCDRHGRFIEEPGLDRLEPGVSREADV
                     RAVFGQPDAIIPGPNGTRIMQYPRGPEGPRTWFFELDSEGILRGYRQVLTPENFSRIR
                     PGMSRDEVRMELGRPGSTTPFPLKNEEVWDWRYLQDNNPQFFSVHFDRTTGQVVRTST
                     SPANGEVPMR"
     gene            2553..4286
                     /locus_tag="VM406_00115"
     CDS             2553..4286
                     /locus_tag="VM406_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HVL74389.1"
                     /translation="MGYVNYLLLLGAALMFFGIVFGASSSRFGVPFLLIFLVVGMLAG
                     EDGFGGIVFHDYQLSFLVGNLALAIILLDGGLRTKMQTFRVALKPSLALATFGVVVTA
                     GLVGLFAAWVLGLDWRVALLLGTIVGSTDAAAVFSLLKASGTRLNDRISNTLEIESGI
                     NDPMAIFLTITLIELLTHTSGALSIPAMLQQLALQFSIGLAFGIGIGYLLGAVVSRIH
                     VGEGLHALLLCSGGVALFGLTNLVGGSGFLAIYLAGLIVGNSRYRVGENVMRAMDGMA
                     WLAQSGMFLLLGLLATPKEVWDIAGPALAIAIFLMLVGRPVAVILSLLPFRFSMKEVG
                     FISWVGLRGAVPIVMAMFPLLAGIEGALLIFNVAFMVVLASLLLQGTSVPFLARALGI
                     SMPERAEPVLRTQLRGAGEKGYEMAQFEIARHSAMLGAHAGTLTMPPDAHIVNVLRNG
                     TLLRADEAGALLVDDVVTVVCAEAALDRLGEMFHGRHSPGRTMREFYGDFMLDGDARL
                     ADVAALYGNMEEHPELQALTLGEAIERRLRRAPVEGDSVEVCSLLLTVRAMEGRVVTK
                     VGMKLPKIKSE"
     gene            complement(4378..5331)
                     /locus_tag="VM406_00120"
     CDS             complement(4378..5331)
                     /locus_tag="VM406_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVL74390.1"
                     /translation="MTITIARSHQIYSVKEVESAQEKARAMNAPELEAFYEDMITLGA
                     ERFVTTPSSTSALEPLYEDCPNFEEVLDDLKRYVALALAGGAGVNLMPVLLLGDPGVG
                     KTHFGKRLAAALGTEFEFISMNALSAGFVITGSSSTWKGARCGKVAERLVRGKFANPV
                     VLLDEVEKATGSTQSDPMAALYQLLEPETSRAFHDEFIDVDLDASQVFWVLTANSLDG
                     IPPALLSRMAVYEVPPPTREQAAGIAQRVYAGLLRELKLQNLEEELSDNVLDKLADVS
                     PREMRKALLDGLGFAVQSGRSALVPEDVRVRGEQGRRRIGF"
     gene            complement(5539..6900)
                     /locus_tag="VM406_00125"
     CDS             complement(5539..6900)
                     /locus_tag="VM406_00125"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVL74391.1"
                     /translation="MQLQFLGATGTVTGSKYLIEAGRARALVDCGLFQGYKQLRLRNW
                     ADLPIAPSGIHAVVLTHAHLDHSGYLPLLVRNGFRGKVRCTEATYELCRILLPDSARL
                     LEEEAAYANRKGYSKHQPALPLYTVADAERALRQFEPVEFDRSFVIAGSLLGRFARAG
                     HILGAAMVTIEDGLRSICFSGDLGRSQDVLMPPPDIVRAADYLVLESTYGDRLHDRTD
                     PMVVLAKTIKDTAARGGVVVIPAFAVGRAQELIYYMHRLRESGAIGPDLPIYLNSPMA
                     ADVTDLYSRYHRDHRLSAEACRAMCESVRIVNSVEESITLNRQRMPMVIIAASGMATG
                     GRVLHHLKAFAPDPRNTILFAGFQAGGTRGAAMLAGADAIKIHGEYVPVRAKVVQIEN
                     LSAHADADEILAWLRNFETAPREVFLTHGEPHAADVLRRRIEEELHWRCRVPEYLEGV
                     ALR"
     gene            7231..9180
                     /locus_tag="VM406_00130"
     CDS             7231..9180
                     /locus_tag="VM406_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HVL74392.1"
                     /translation="MTEQTDTSRTDQQQALIDSLIAFTKDHRAVYWSGTLSEFLERVM
                     TADPRGAARTSHQYMWDMIRWMGCEDENGMFRCKLFDQELFGVDEAIGRVVDYFKAAA
                     AGSEVGRRLLLLLGPPSGGKSTLVILLKRGLEEYSRTEEGALYAIQGCPVHESPLHLV
                     PHSLRPRFRDTYGVDITGEICPHCRSRLESEYQGDFLRMPVERIFISEAGRVGIGTYA
                     PHDPTTADLADLVGSVDLSKVAEYGDEGDPRAWSWSGAVYAGSRGMLEMIEILKVKRE
                     FLYLLLTLTQEKNVKVSRFPLIYLDETILAHTNLAEFRKFLQESENEALLDRMVIIQV
                     PYTLNYKDEARIYKKLISSAAPAFRQVHLDPHVLHAAAVFAILTRLQDADERELELGK
                     KVRLYANEEVEGYAHVDAERMKQRMPEEGLSGISPRFVINALSNAIIQSNAKSLTTMD
                     VLLALKDAIDNDARIDAKRKRKWIDYLVLARKDFYNRWVKEDVHKALFVSFEQEAQDL
                     LDKYLDEVEATLDNRQIKDPITNEERKPDERFLRAVEEKIHISDSGKQSFRQEVVRKA
                     MGAFKRGEKFTLDSHAQLHNAVQQYLFEQRRDVLRLVSSTTRPDEEVRQKISVVEKRL
                     VDEYGYDAHSAREALNYVTTLMAQE"
     gene            9203..10372
                     /locus_tag="VM406_00135"
     CDS             9203..10372
                     /locus_tag="VM406_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HVL74393.1"
                     /translation="MNDRLTLSAETPWYDLFSRGSRDWLRHNQKVREAVQQNLPDLVA
                     GPDVITGGQDKTVQVPVRLLEHARFRLSDSSHTQSSAGQGQGKPGDVLRQAQPQSGED
                     EGEGGNNEGEVKLLLEFKVEDIIDWLWEELQLPDLQPKRNTAVTDDEYVREGWDKHGA
                     RARLDRRRTVKEAVKRRATQERMQANPVPFTNEDLRFKQLVQRRRPATNAVVIFALDV
                     SASMTEAERKLAKSFFFFVLQGIRRRYGKVETRFIAHTTQAWEFSENDFFSVSGTGGT
                     GASTAFKLALELIENEYDPSQYNGYLFYASDGENFTEDRGAATDYLARLGKLLNFIGY
                     VETVPGSPRTTETEMKMLCGEVQRSGAPISSAVLARHDDVWNAIRKFFTEQSVER"
     gene            10439..11812
                     /locus_tag="VM406_00140"
     CDS             10439..11812
                     /locus_tag="VM406_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HVL74394.1"
                     /translation="MIAQLKQYSAQIEALARKLGLDYYPVDFELVPNNFMMEIAVYGL
                     PVRMHHWSFGVRYIHQLIRQGMGHSRIFEVMFPGDPCRAYLVNGNTLPENTLVTAHVL
                     GHADFSKNNHLFAKFMEMAGGHILEQAAARAHRIETAIQEHGQARVEAVLDAALALEP
                     HIDVTRELHRPLYPTEPVQEASPAPVDPFLQRYRALPGERLQETAGNAQRRMQIPPQP
                     EYDLLWFIAHYAPEMEDWERDVFLAVREESFYFYPVFACQIMNEGWASYWHARLLREA
                     DFLPHDLYLSAIKAHSDVVRPYAAERQLALAVNPYHLGFSMWEDIIEKHGLEKARQIM
                     REEDDFGFVRNYLTRDLIEKLELFVYEARDDGDIRIASADVHAVHEAILSPKYNFGAP
                     RIAVNHLHVDGSLQLLHDHQTDGRGIDLGRAERVLEYIARVWRRPVTLHTVGDRGEAR
                     VVTARRH"
     gene            complement(11809..13509)
                     /locus_tag="VM406_00145"
     CDS             complement(11809..13509)
                     /locus_tag="VM406_00145"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVL74395.1"
                     /translation="MHGPSLRRQLVLPFVLLVLFVSVAIGWVSFRAGAEAVQNLTERV
                     VMDLLNRLGNATERHLEAAVSAMDALRDGGGAQEAGADLQDFSALEARMWMVTRLSST
                     IDSLFYVSNEDGRFLGVRRLSDGSAEVLLRPPEGGPLHVYSASAPGRRERLLRVEEFE
                     PRQRTWYAVAAQAGEPVWTPLYQDDEGAGPTVALAAPVPRIDGTLAGVLAADFDLQAL
                     TAFMQQLPISSHGIAFIVDAQGAVIATSGHERPFAIASGVVRPLKVAEMDTPILRSAW
                     SRVGAMVRSEDGGRSGPWMTHADGTDIAATRLQNGYGLDWTLVVAVPRSDFMGDVARS
                     VAQAAFIGLVCVILAIVFGLSVLNRVLRDIRVLSEAARRVGEGEPLPALNIHRRDELG
                     QLAASFSEMEYGLRIDKLTAVFNRASLAAQIAAHRRQLDQKPGEKPAFALLFVDLDYF
                     KKVNDQHGHAAGDRVLVAVAARLKESVRVSDVVARYGGDEFVILLKGVTDVEDVMRMR
                     EKIRSVVEQPVRLDVITVTVGASVGWALFPEDGKDVDKLLKIADSRMYDTKRRRHAVA
                     "
     gene            13749..>14254
                     /locus_tag="VM406_00150"
     CDS             13749..>14254
                     /locus_tag="VM406_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74396.1"
                     /translation="MKKVSALLAALLASLALAACEQEGAGTGATGDTTAPQAETAAGM
                     PPATTPAEPAAEPAAEPASPGMADQQPGMTQPETGAAPGAEPAPGAAPGTTGDTGTTG
                     AAGAGTTGDAGTTGAAGAGTSGDTGMSGAAGAGTSGDTGTTGATGTGTDTGTGAGTGA
                     GTGAGTQQT"
BASE COUNT         2577 a         4656 c         4484 g         2537 t
ORIGIN      
        1 actgcatgcc gttcatcgga gggcatgacg atgaacggca tgcagtggat gttcgaaacg
       61 atggatgcat ggcgccggcg ccagggaaat gcgttcgacc ttgcggggct gggaccgcgg
      121 gaagtcgatt gccagatcga cctggacatg cctgcattgc gcctgcgctg ctacggcgga
      181 cccgaagcgc gtgccgtcct cgccgttccg gcaccgatca agcgtcacta catctgggat
      241 ctgctgcccg actgcagcgc agtccgacgc ctgctggacg ccggctatcg tgtctacctg
      301 attgaatgga aggatccgca aggcgaaatg gccgaagccg ggctgcagca gtacggcagc
      361 gactggatcg atgcctgcgt gcagcggata cgccaggact gtccgcagac gcccctcgtc
      421 ctgatcagcc attcgctcgg cggtatcttt gccaccatct atgcgtcgct gcaccccgag
      481 caggtacggg ctcttgtgct gctcgaagtc ccgctgcact ttggcaaggc cagcggcgcg
      541 tttcttccgc tgctgctggc tgcgccgcct accccgctgc tgctgcggga catgacgcgc
      601 gtgcccggct cgctgctgag catggtgagc gccatggctt ctccccgcac ctttcacggc
      661 gagcgcgccg ccgacttcgc agccagcctt gcctctcccg ccgacctgca acgccacatg
      721 ctggtcgagc gctgggcact ggatgaggcg ccgatggcca acttactgtt cgagcagatc
      781 gtggaagacc tgtaccgcaa tgacaagctg atgcggggga cgctggaaat ctccggaacc
      841 acgatcggcc ccgcgcagat gcgtgtgccg ctgtttgccg tgcatgaccc ggcaagcgac
      901 atcattccgt cagagtcggt gaccacctac gtcgatgcgg cggccagcga cgacaaggcg
      961 ctcgccaccg tcgaatcgga gcgcggcacc ggcctgtccc atgtggcggc cctggtgggg
     1021 ccgcgcgcgc acgcgctgct ctggccgcgc atcctgtcgt ggctccgaaa tctgcccgag
     1081 cgccgcacgg gcgcataaaa aacaggctgt cccgccaagg gcagcctgct tgagttgcgt
     1141 ctcaacctga tgcttactgc tgttgctggc gcggcgagtc ctgatggagg ccgccgctgg
     1201 tgccggcacc ggctgtaccc gtagtgtcgg tggtgccagt cgcgccaggc gcatcgatgc
     1261 ccgtacgggc ggcgccgtcg gtgccggtgg cgccggtcgt tgcgtcggtg ccggtcgcgc
     1321 cggttgttgc atcggtgccg gtggcgccgg tcgttgcatc ggtgccggtc gcgccggtcg
     1381 tcgcatcggt gccggtggcg ccggtcgttg catcggtgcg ggtcgcgccg gtcgttgcgt
     1441 cggtgccggt tgcgccggtc gttgcgtcgg tgccggtcgc gccgctggca tcacggctgc
     1501 gcccgttgcg ctcttgatcg cgctcacgtg cggccccagc cgcgccttca cccgccgctc
     1561 ccgcgccggt caccccggtc tcgccgcttg cctgcccggg ctgctgatcc tgctgacggg
     1621 caatgatctc ggacgcgcgc tgctcgactt ccgcgaccac ggccggatcg gtgctctccc
     1681 ggatttcggt ttgcggcggc tgtcccgtcg ttgcgaacgc cgcactgctg acgaacaacg
     1741 ccgaaatgaa tgctgccaga gatttctttt gcatctagac ctccttccat tgcgaaattt
     1801 cacgaatgcc ggcaagcgac agcctgccga actggcgaac tttgcaatac gacgctcaaa
     1861 acgccgcttc ggtttcgtga ttggccgagg gagatgtaac ttaatgattc cccatcaacg
     1921 catcggcacc tcgccgttag ccggcgaggt ggatgtgcgc acgacctgcc ccgttgtcct
     1981 gtcgaaatgc acgctgaaaa attgcgggtt gttgtcctgc agatagcgcc agtcccacac
     2041 ctcttcattc ttaagaggga aaggcgtggt gctgcccggg cgccccagtt ccatcctcac
     2101 ttcgtcgcgg ctcatgccgg ggcggatgcg ggaaaaattc tccggcgtca ggacctgtcg
     2161 gtagccgcgc aatatccctt cgctgtcgag ctcgaagaac caggtgcgcg gcccttccgg
     2221 cccgcgcgga tactgcatga tgcgcgtgcc attcggtccg ggaatgatgg cgtccggctg
     2281 accgaatacc gcgcgcacat cggcttcccg ggaaacgccc ggctccagcc ggtccagtcc
     2341 cggctcctcg ataaaacgcc cgtgccggtc gcaaccgaca agcatcagga aaaacagaac
     2401 ggcaaatgcg ggatacaggg acttcatcgc ggctcgtggc aggaagcgga gttccttgac
     2461 tgatcgtcct gcaaacaatt ggtttcatga aagcggattg ccacaacggc acggtacaat
     2521 ccactggcaa aaaaacgaca ctacaaacat agatgggcta tgtgaattac ctgctgctgc
     2581 tcggcgcggc gctaatgttc ttcggaatcg tctttggcgc ctcgtcgtcc aggttcggcg
     2641 ttcccttcct gctgatcttt ctggtggtcg gcatgctcgc gggcgaggat ggctttggcg
     2701 gtatcgtttt tcacgactat cagctcagct tcctcgtcgg aaatcttgcg ctcgccatca
     2761 tccttcttga cggcgggcta cgcacaaaaa tgcagacctt ccgggtggcg ctgaaacctt
     2821 cgctggcctt ggcaaccttt ggcgtcgtgg tcaccgcagg cctggtcggg ctgttcgccg
     2881 cgtgggtgct cggcctcgat tggcgcgttg cgctgctgct tggcaccatc gtgggttcca
     2941 ccgatgcggc cgcggtgttt tccctgctga aagccagcgg cacgcggctc aacgatcgta
     3001 tttcgaacac gttggagatc gagtcgggca tcaacgaccc gatggcgatc tttctgacca
     3061 ttacgctgat cgagctgctg acccacacct cgggcgcgct gtcgattccc gccatgcttc
     3121 agcaactggc gctgcagttc agcatcggtc ttgcgttcgg tatcgggatc ggctatctgc
     3181 tgggtgcggt cgtaagccga atccatgtgg gtgaaggcct gcatgcgctg cttctgtgct
     3241 ccggcggcgt cgccctgttc ggcttgacca acctggtcgg cggaagcggt tttctcgcga
     3301 tctacctggc cgggctgatc gtcggcaaca gcaggtaccg tgtcggcgag aatgtcatgc
     3361 gggcaatgga tggcatggcc tggctcgcgc aatcggggat gttcctgctg ctcggcctgc
     3421 tggcgacgcc gaaggaggtc tgggacattg caggcccggc attggcaatt gcgatcttcc
     3481 tgatgctggt gggccgaccg gtggctgtca tattgagtct gcttccattc cgcttcagca
     3541 tgaaggaagt gggcttcatt tcctgggtgg gcctgcgcgg cgcggtgccg atcgtgatgg
     3601 cgatgttccc gctgctggcc ggtatcgaag gcgcactgct catcttcaac gtggccttca
     3661 tggtggtgct cgcgtcgctg ctgctgcaag gcacctcggt tcccttcctg gcccgcgcgc
     3721 tcggcatcag catgcccgag cgggccgaac cggtgctgcg cacccagttg cgcggcgccg
     3781 gggagaaagg ctatgagatg gcgcagttcg agatcgcgcg ccactccgcg atgctgggcg
     3841 cccacgccgg cacgctgacg atgccccctg atgcgcatat cgtcaacgtg ctgcgcaacg
     3901 gcaccttgct acgggcggac gaagccggcg ccctgctggt cgacgacgtg gtcaccgtcg
     3961 tctgcgcgga agccgcgctg gaccgcctcg gggaaatgtt ccatgggcgg cacagccccg
     4021 gacgcacgat gcgcgagttc tacggcgact tcatgctcga cggcgatgcg cgattggccg
     4081 atgtggcggc gctgtacggc aacatggaag aacaccccga actgcaggcg cttaccttgg
     4141 gcgaagcgat cgagcgtcgc ttgcgccggg cgccggtgga aggcgattcc gtggaggtgt
     4201 gcagcctgct cctgaccgtg cgcgcaatgg aaggccgggt ggtcaccaag gtcgggatga
     4261 agctgccgaa gatcaagtcc gaataagcac cccgcgtctg gccgcactgc gcggactgtc
     4321 agcggcgcgg cgccttgcct tcggcattag tcggggtttc cttgcgccgg caaaccgtca
     4381 gaaccctatc cggcggcgcc cctgctcgcc gcgcacgcgt acgtcttccg gtaccagcgc
     4441 gctccgtccg gactgcaccg cgaatcccag tccatcgagc aaggccttgc gcatctcgcg
     4501 cggcgacacg tcggccagct tgtcgagcac gttgtcggac agttcctctt ccaggttctg
     4561 cagcttcagt tcgcgcaaca gacccgcgta cacgcgttgc gcgatgccgg ccgcctgctc
     4621 ccgtgttggc ggcggcactt cgtagacggc catgcgcgac agcagcgctg gcggaatgcc
     4681 atcgagactg ttggcggtca gcacccagaa cacctggctg gcgtcgaggt cgacatcgat
     4741 gaactcgtcg tgaaacgcgc gcgaggtttc cggctccagc agctggtaca gcgccgccat
     4801 cggatcggat tgggtggaac cggtcgcttt ctcgacctcg tcgagcagca cgacgggatt
     4861 ggcgaacttg cctcgcacca ggcgttcggc cacctttccg cacctggcgc ccttccaggt
     4921 ggaagaactg ccggtgatca cgaaaccagc cgacaaggcg ttcatgctga tgaactcgaa
     4981 ctcggtgcct agcgccgctg caagccgctt gccgaaatgc gtcttgccga cgccgggatc
     5041 gccgagcagc agcaccggca tcagattgac ccccgcgccg cctgccagcg ccagcgcgac
     5101 atagcgcttc agatcgtcca gcacctcctc gaagttcgga caatcttcat acagcggttc
     5161 gagcgcgctg gtgctcgatg gcgtcgtaac gaagcgctca gcgcccaggg tgatcatgtc
     5221 ctcgtagaac gcctccagtt ccggggcatt catcgcacgc gccttttcct gcgcgctttc
     5281 cacttccttc accgaataga tttgatgaga gcgggcgatc gtgatcgtca tgcgcaaatc
     5341 tcctcaatct gctgtgcggt gcatgcatcc gctgaagcgc tcgaagcaat ctccgttcca
     5401 ccggaaaaat ctttccttcc ggacgtgaag atttcagcga aaacaccggc ggccgtcaac
     5461 cttgcagcgc gcagctgtcg cgcgccgcaa acacggcaca ccgtcgctgg gcgccacgtt
     5521 cgccttgccc gcatcgcgtc agcgcagtgc aacgccctcc aggtattccg gcacgcggca
     5581 gcgccagtgc agttcctcct cgatccgacg gcgcagcaca tcagcggcgt gcggttctcc
     5641 gtgcgtgaga aacacctcgc ggggcgcggt ttcgaaattg cgcagccagg ccaggatctc
     5701 gtccgcatcc gcgtgggcgg acaggttctc gatctgcacc actttcgcac gcaccggcac
     5761 gtactcgccg tggatcttga tcgcgtcggc gccggcgagc atggccgctc cccgtgtacc
     5821 ccctgcctgg aagccggcga acaggatggt gttgcgcgga tccggtgcaa acgccttcaa
     5881 gtggtgcagc acccgtccac ctgtcgccat gccgcttgcg gcaatgatga ccatcggcat
     5941 gcgttgccgg ttcagcgtga tcgactcctc gacgctgttg acgatgcgca ccgattcgca
     6001 catcgcgcgg caggcctcgg cggacagccg gtgatcccga tggtagcggc tgtagaggtc
     6061 ggtgacgtcg gccgccatgg gactgttgag atagatcggc agatcagggc cgatcgcgcc
     6121 ggattcgcgc agccgatgca tgtaatagat cagctcctgc gcccgcccca ctgcgaaggc
     6181 cgggattacg accacgccgc cgcgcgcggc ggtgtccttg atggtcttgg ccagcaccac
     6241 cattggatcg gtacggtcat gcaggcggtc gccgtaggtc gattcgagca cgaggtaatc
     6301 ggcggcgcgc acgatgtcag gcggcggcat cagcacatcc tggctgcgtc ccaggtcgcc
     6361 ggagaagcag atggagcgca agccgtcctc gatcgtcacc atcgccgcgc cgaggatgtg
     6421 gccggcgcgc gcgaaacgcc cgagcaagga tccggcaatc acgaagctcc tgtcgaattc
     6481 caccggttcg aactgccgca gcgcgcgctc ggcatcagca accgtgtaga gcggcaaggc
     6541 cggctgatgc ttcgaatagc ccttgcggtt tgcatacgcc gcctcctcct cgagcaggcg
     6601 cgcgctgtcg ggcagcagga tgcggcacag ctcataggtt gcctcggtgc accgcacctt
     6661 gccgcggaac ccgttgcgca ccaggagcgg caggtagccg ctgtggtcga gatgagcatg
     6721 ggtgagcacc acggcatgaa tgccggaagg cgcaatcggc aggtccgccc agttgcgcag
     6781 ccgcagctgc ttgtatccct ggaacaggcc gcagtcgacc agcgcgcgcg cacggccggc
     6841 ctcgatcagg tacttggaac cggtgacggt gccggtggcg ccgagaaact gaagctgcat
     6901 ggaatccttc ctgaaggctt ttttctatcg ccactatccg catcggcttc gcttgcgccc
     6961 ttgatctgag tcaattttcg ccctggcgca gcaccggtgc gccctccggg cgcagtgctc
     7021 gcacgcattt tgcatccccg gcttgcatgg caccgaagct ggcacagaga cacggggcgc
     7081 aagccggcac cattgattgc gctcatgatc cgcgcttgca cgcgcggcct acactggcat
     7141 caggcgtatg aatgtcgaac ggaacagaac cgtgaacctg aatcgggtgt ccatcgtaaa
     7201 gagcatccgg ttttctgatg gggggacagc atgaccgaac agaccgacac cagccgtacc
     7261 gaccagcaac aggcattgat cgacagcctg attgccttca ccaaggacca tcgcgcggtc
     7321 tactggtcag gtaccctttc cgaatttctc gaacgcgtca tgacggccga tccgcgcggt
     7381 gccgcgcgca cctcgcacca gtacatgtgg gacatgatcc gctggatggg ctgcgaggat
     7441 gagaatggca tgttccgctg caagctgttc gaccaggagt tgttcggggt cgatgaggcg
     7501 atcgggcggg tggtcgatta cttcaaggca gcggcggcag gctcggaagt cggccgccgc
     7561 ctgctgctgc tgctcggccc gccttccggc gggaagtcca cgctcgtgat cctgctaaag
     7621 cgcggactcg aagagtacag ccgcaccgag gaaggggcgc tgtacgcaat ccagggatgc
     7681 ccggtgcatg aatcgccgct gcatctcgtc ccgcattcgc tgcgcccgcg ctttcgcgac
     7741 acctacggcg tcgacatcac cggcgaaatc tgcccgcatt gccgcagccg cctggagagc
     7801 gaataccagg gagatttcct gcgcatgccg gtcgagcgca tcttcatctc cgaagcgggc
     7861 cgtgtcggaa tcggcaccta cgcgccgcac gatcccacca ctgccgatct cgccgacctc
     7921 gtcggctccg tggatctctc caaggtcgcc gagtacggcg acgagggcga tccgcgcgcc
     7981 tggtcgtggt cgggtgccgt gtacgcaggc agccgcggca tgctcgagat gatcgagatc
     8041 ctgaaggtca agcgcgaatt cctctacctg ctgcttacgc tgacgcagga aaagaacgtg
     8101 aaggtctcgc gcttcccgct gatctatctg gacgagacca tcctcgcgca caccaacctc
     8161 gcggaattcc gcaagttcct ccaggagagc gagaacgaag ccctgcttga ccgcatggtc
     8221 atcatccagg tgccgtacac actgaattac aaggatgaag cacgaattta caagaagctg
     8281 atctcctctg cggccccggc cttcaggcag gtgcacctgg atccgcacgt actgcatgcg
     8341 gcggcggtat ttgcaatcct tacccgtctt caggatgccg atgagcgcga gcttgaactc
     8401 ggcaagaagg tgcgcctgta tgcgaacgag gaagtcgagg gctatgcgca cgtcgacgcc
     8461 gagcgaatga agcagcgcat gcccgaggaa gggctgtccg gcatttctcc acgtttcgtg
     8521 atcaatgcgc tgtcgaacgc aatcatccag tccaatgcga agagcctgac cacgatggat
     8581 gtgctgcttg ccctaaagga tgcgatcgac aacgacgcgc gcatcgacgc caagcgcaag
     8641 cgcaagtgga ttgactacct agtcctcgcg cgcaaggact tctacaaccg ttgggtcaag
     8701 gaagacgtgc acaaggcact gttcgtctcg tttgaacagg aggcccagga cttgctcgac
     8761 aagtatctgg acgaagtcga agcgacgctc gacaaccgcc agatcaagga tccgatcacc
     8821 aatgaggaac gcaagcccga cgagcgcttc ctgcgcgcgg tggaagagaa aatccacatc
     8881 tcggattcgg gcaagcaatc cttccgccag gaggtggtgc gcaaggccat gggcgcattc
     8941 aagcggggcg agaagtttac gctggacagc catgcccaac tgcataacgc ggtacagcag
     9001 tatctgttcg agcagcgccg cgacgtgttg cgcctggtca gctccaccac ccgcccggac
     9061 gaggaagtgc gtcagaaaat atcggtggtg gagaagcgcc tcgtggacga atatgggtac
     9121 gatgcgcata gcgcccgcga agcgctgaat tacgtgacca ctctgatggc ccaggagtga
     9181 gtgacgcacc ggagatgcat gcatgaatga ccgactcacc ctgtctgcag aaacgccgtg
     9241 gtacgacctg ttctcgcgcg gctcgcggga ctggttgcgc cacaaccaga aggtacgcga
     9301 ggcggtgcag cagaacctgc cggacctggt cgcgggcccg gatgtcatca ctggcggaca
     9361 ggacaagacc gtgcaggtac cggtgcgcct gctcgaacat gcacgctttc gcctgtccga
     9421 ttcctctcat acgcagtcca gcgccggcca ggggcagggc aaacctggcg atgtgctgcg
     9481 ccaggcgcag ccgcagtccg gcgaggacga aggcgaaggc ggcaataacg agggcgaagt
     9541 caagctcttg ctcgagttca aggtcgagga catcatcgac tggctgtggg aagagctgca
     9601 gcttcccgat ctgcagccca agcgcaatac cgccgtcacc gacgacgaat atgtgcgcga
     9661 aggctgggac aagcacggtg cgcgcgcgcg ccttgaccgc cgccgcacgg tgaaggaagc
     9721 ggtcaagcgg cgcgcgacgc aggagcgcat gcaggccaat ccggtgcctt tcaccaacga
     9781 agacctgcgc ttcaagcagc tggtgcaacg ccgccggcct gcgaccaatg cggtggtgat
     9841 cttcgcgctg gacgtctcgg catcgatgac ggaagccgag cgcaagctcg ccaagagctt
     9901 cttcttcttc gtgctgcaag gcattcgccg ccgctacggc aaggtggaaa cgcgcttcat
     9961 cgcacatacc acgcaggcct gggaattctc cgagaacgat ttcttctcgg tcagcggcac
    10021 cggcggcacc ggcgcgtcca cggcgttcaa gctggcgctg gaactgatcg agaatgaata
    10081 cgacccgtcc cagtacaacg gctacctgtt ctatgcgtcc gacggagaga acttcaccga
    10141 ggaccgggga gcggcgacgg attacctcgc gcggctgggc aagctgctca acttcatcgg
    10201 ctatgtcgaa accgtaccgg gctcgccgcg cacgacggag accgagatga agatgctgtg
    10261 cggcgaagtc cagcgcagcg gcgcgccgat ctccagcgcc gtgcttgcgc gccacgacga
    10321 cgtctggaac gccatccgca agttcttcac ggagcagtcg gtcgagcgct gatcggcatt
    10381 ccgcctttcc tttcctcgag ggcaacagct catgtgcacc accacgcaat caccagaaat
    10441 gatcgcccag ctgaagcagt actctgcgca gatcgaggcg ctcgcgagaa agctcgggct
    10501 ggactactac ccggtcgact tcgagctggt gccgaacaac ttcatgatgg agatcgcggt
    10561 ctacggcctg ccggtgcgca tgcaccactg gtcgttcggc gtgcgctaca tccatcagct
    10621 gattcgccag ggcatggggc attcgcgcat cttcgaggtg atgttccccg gcgatccctg
    10681 ccgcgcctac ctggtcaacg gcaacacgct gccggagaac acgctggtca ccgcgcacgt
    10741 gctcggccac gcggacttct ccaagaacaa ccatctgttc gcgaagttca tggaaatggc
    10801 cggcggtcac atactggaac aggcggccgc gcgcgcccat cggatcgaaa ccgcgatcca
    10861 ggagcacgga caggcccggg ttgaagccgt gctcgatgcc gcgctcgcgc tggaaccgca
    10921 catcgacgtc acgcgcgagc tgcaccgccc gctctaccct accgagccgg tgcaggaagc
    10981 ttcaccggcc ccggtcgatc ccttcctgca gcgctaccgg gcgctgcccg gcgagcggct
    11041 tcaggaaaca gcgggcaatg cgcagcggcg catgcagatc ccgccgcagc ccgaatacga
    11101 cctgctgtgg ttcatcgccc actacgcacc ggagatggaa gactgggaac gcgacgtgtt
    11161 tctcgcggtg cgcgaggagt cgttctactt ctatccggtc ttcgcctgcc agatcatgaa
    11221 cgagggctgg gcctcgtact ggcatgcgcg cctgctgcgc gaagcggatt tcctgccaca
    11281 cgacctctat ctttccgcga tcaaggcgca ttcggacgtc gtgcgtccgt atgcggcgga
    11341 gcgtcagctg gcgctggcgg tgaatcccta ccaccttggt ttttcgatgt gggaagacat
    11401 catcgaaaag cacggcctcg agaaggcgcg gcagatcatg cgcgaggagg acgatttcgg
    11461 cttcgtgcgc aactatctca cgcgcgacct gatcgagaag ctggagctgt tcgtctacga
    11521 ggcgcgcgac gacggcgaca tccgcattgc cagcgccgac gtgcacgcgg tgcacgaggc
    11581 catcctctca ccgaaataca acttcggcgc gccgcgcatc gcggtcaatc atctgcacgt
    11641 ggatggcagc ctgcagctgc tgcacgacca tcagaccgat ggccgcggca tcgatctcgg
    11701 ccgcgccgaa cgggtgctcg aatacatcgc ccgcgtctgg cggcgtccgg tcacgttgca
    11761 caccgtgggc gaccgcggcg aggcacgcgt ggtgaccgcg cggaggcatt aggccaccgc
    11821 atggcggcgg cgcttggtgt cgtacatccg gctatctgcg atcttcagca gcttgtcgac
    11881 atccttgccg tcctccggaa acagcgccca tccgaccgat gcgcccacag tgacggtgat
    11941 gacgtccagc cgcaccggct gttccaccac gctccggatc ttttcacgca tccgcatcac
    12001 gtcttcgacg tccgtcaccc ccttgagcag gatgacgaat tcatcgccgc cgtagcgcgc
    12061 gaccacgtcg gacacgcgca cggactcctt caggcgtgcc gccaccgcca ccagcacgcg
    12121 gtctcccgcc gcatggccgt gctggtcgtt gaccttcttg aagtaatcga gatcgacgaa
    12181 caggagcgcg aaggccggtt tctcgccggg cttctgatcg agctggcggc ggtgcgcagc
    12241 gatctgcgcg gcgagcgatg cgcggttgaa caccgcggtg agcttgtcga ttcgcagccc
    12301 gtactccatt tcgctgaatg aagcggcgag ctgcccgagt tcgtcacgcc ggtggatgtt
    12361 cagggcaggc aaaggctcgc cctctcccac gcggcgcgca gcctcgctga gaacgcggat
    12421 gtcgcgcaag acccggttga ggacagacag gccgaacacg atggcaagta tcacgcacac
    12481 caggccgatg aaggcggcct gggcgacgct gcgagcgaca tcgcccatga aatcgctgcg
    12541 gggcacggcc accaccagcg tccagtcgag cccatatccg ttctgcagac gcgtggcggc
    12601 gatgtcggtg ccgtcggcat gagtcatcca cggcccgctg cggccgccgt cctcgctgcg
    12661 caccatggcg cccacacgcg accacgcgct gcgcaggatc ggcgtatcca tctccgccac
    12721 tttcagaggc cgcaccacgc cgcttgcgat ggcaaaaggc cgttcgtggc cggaggtcgc
    12781 aatcaccgcc ccctgggcat ccacgatgaa ggcaatgcca tggctgctga tcggcaattg
    12841 ctgcatgaac gcggtcaggg cctgcaagtc gaagtcggcg gccagcacgc cggccagcgt
    12901 gccatcgatg cgcggcaccg gcgccgccag ggcgacggtc ggcccggccc cctcgtcgtc
    12961 ctggtatagc ggcgtccata ccggttcgcc ggcctgagcc gccaccgcat accatgtccg
    13021 ctggcgcggc tcgaactcct cgactcgcag caggcgctcg cgcctgccgg gagcgctggc
    13081 cgaataaaca tgcagcggtc cgccttcggg cgggcgcagc agcacttcgg cgctgccgtc
    13141 gctcaacctg cgcacaccga gaaaacgccc gtcctcgttg ctcacgtaga aaagactatc
    13201 gatcgtcgac gaaaggcgcg tgaccatcca catacgcgcc tcaagcgcgg aaaaatcctg
    13261 caggtccgct cccgcttcct gcgctccgcc tccgtcgcgc agcgcatcca tcgcggagac
    13321 cgcggcctcc aggtgccgct cggtcgcgtt cccaagccgg ttgagcagat ccatcaccac
    13381 gcgttcagtg aggttctgca ccgcctccgc gcccgcacga aacgagaccc agccgatggc
    13441 gaccgaaacg aacagcacga gcaggacgaa aggcaacacg agttgcctgc gcagcgacgg
    13501 accgtgcatg gaatgggcgg gacgggaaat gtttgcgacc ggaaattata gccgggcagc
    13561 aagattagct tgcaattacc gcacgtcgtg atgaacggca atcgaaatcc cggagttgaa
    13621 agcttgaacg aaatatatcg tcgtttgatt tttatcaagc gcttaagggg cacttaagcc
    13681 ggccggcttt ctcaaaccta gcacaacaca cctgcatgac cgaataactt tatgaaaggg
    13741 aaaatctcat gaaaaaagtc tccgcactgc tcgccgcgct gctcgcttct cttgctcttg
    13801 ccgcctgtga acaagaaggt gccggcaccg gcgccaccgg cgacacgacc gcgccgcagg
    13861 ccgaaaccgc agcaggcatg ccgcctgcca cgactccggc cgaaccggct gccgagccgg
    13921 ctgcagagcc ggcctcgcca ggcatggccg accagcagcc cggcatgacg caacccgaaa
    13981 ccggcgcggc acctggcgct gaacctgctc ctggcgcggc gcctggcacg accggcgata
    14041 ccggcacgac gggcgctgca ggcgcgggga cgaccggtga cgccggcacg accggggctg
    14101 caggcgccgg tacctcgggt gataccggca tgagcggcgc cgccggcgcc ggcaccagcg
    14161 gcgataccgg cacgaccggc gccaccggca cgggtaccga cacgggcacc ggtgctggaa
    14221 ccggcgcagg aacgggcgcc ggcactcagc agac
//
LOCUS       DATLGF010000006         6307 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_79429,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000006 DATLGF010000000
VERSION     DATLGF010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79429"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="VM406_00155"
     CDS             <1..1188
                     /locus_tag="VM406_00155"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HVL74397.1"
                     /translation="VYDAIRTPRGKGKKDGSLHTVKPVNLLTGLMHDLQSRHRLDTAL
                     VDDVVLGCVTPVGDQGACIAKTAALAAGWDWKASGMQLNRFCASGLEAVNLAAQKVRS
                     GWEDLVVAGGVESMSRVPMGSDGGAWAADPETSFATDFIPQGIGADLIATLEGFSRDD
                     VDAFAMRSQHKAAAARAAGRFRSMRPVRDQNGITVLAEDEFIKPSTTMETLAGLKPSF
                     EAPGKMGFDAVAIRKYPQVERIRHVHTAGNSSGIVDGAALALIGSERIGKQLNLTPRA
                     RILATAITGTDPTIMLTGPAPASRKALEKAGLTVDDIDLFEVNEAFAAVVMKFMKDMR
                     VPEEKVNVNGGSIALGHPLGATGCALLGTLLDELEARKLKRGLVTLCVGGGMGIATIV
                     ERV"
     gene            1199..3328
                     /locus_tag="VM406_00160"
     CDS             1199..3328
                     /locus_tag="VM406_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HVL74398.1"
                     /translation="MSIHWNKDTDGIVTLTIDMPGPVNTMNAAFQQAMGDAVARLEAE
                     RDAIKGVIIASAKDTFFAGGDLKELIAIPADGAAQCFEMVGRIKAVLRRLEKLGRPVV
                     AALNGSALGGGLELALACHHRISLDNAKIQFGFPEVTLGLLPGAGGVVRSVRLLGLQE
                     AMPYLVEGKRMAPAEAQAKGFVHALAKDRDDLITQARAWIAANPAPVQPWDADGYKLP
                     GGAPNTPKILPLQQVAPAMLREKTRGNYPAPEAILSAMIEGAQVDFDNALVIEGRYFT
                     QLATGQIAKNMIGTFFFQMQEVGAGKSRPKDVPKWKATKVGILGAGMMGAGIAWANAS
                     RGVPCVLKDVGREQAEKGKAYSAKLLDKRVRQGRISADKASATLNLITPTADAADLAG
                     CDLVIEAVYENRELKAQVTREAAERLAADGVMASNTSTLPITGLAEAFPDPARFVGLH
                     FFSPVDKMQLVEIIRGAKTSEQTLARAYDYVQQIGKIPIVVNDSRGFFTSRVFGTFVN
                     EGIAMLGEGVEPALIENAALAAGMPVGPLAVQDEVALTLMMHVARQTRADVGDSYVPH
                     PAEAIVERMVEEFKRCGKAAGAGFYEYPADGGRKFLWPKLREIFVKPEVQISFEDVRD
                     RMLYIQSIETMRCLEEKVLESTRDANIGSIFGIGFPGWTGGAAQFVNHVGVERFIARA
                     TALQERYGARFAPPRLLIELAESGRTL"
     gene            3652..5178
                     /locus_tag="VM406_00165"
     CDS             3652..5178
                     /locus_tag="VM406_00165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVL74399.1"
                     /translation="MAEDELRGVRAANAELRLQLAALREQGEASKVDAERFRSLIAIS
                     SDWYWEQDADLRFTMRTGWSLGEEVLVRHQYLGLRRWEQPGIAGPVDWDAHRRDLDAR
                     RPFRGLEYRLQIGNEVVWVSINGEPIFGSDGTFLGYRGTGRDISQRKRIEAELRASEE
                     RFHEIVGSMPVGLFIKDPEGRVILMNRECERQWGVRFEQIRNTDGSAFYPRETMNAFR
                     AQDRHAFACRRVIEYEELVSPLDHSRPIIARGMKKPVYDEAGQPRYLIGISIDISAHK
                     RSEEQLRESREALRQLARYQTHVKEEERKRIARDIHDELGQNLMALRLDVGSLINGVS
                     GDGSALSERAKLMMETVDRTIRSVRLIINDLRPAVLDLGLQAAVEWQVAEFMRRTNIS
                     CTLEIDFGEGEPAVEDDVATTFFRVLQESLTNVQRHANASWVRVGLHQDGTCLRLSIA
                     DDGKGGALIRRGKHSFGLLGMRERLKALGGSLEVVSKTGVGTRLTASLPLKAAPLAQS
                     "
     gene            5284..5655
                     /locus_tag="VM406_00170"
     CDS             5284..5655
                     /locus_tag="VM406_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HVL74400.1"
                     /translation="MQTRDVIAALAALAQESRLAVFRLLVQAGPSGMAASRIAEQVGI
                     APSSLSFHLKELTHAGLVTPSPEGRFVIYRANFDTMNGVLAFLTDNCCGGNPCAPVAA
                     CVPEQPIEFKKRGSKHAREDV"
     gene            5636..6136
                     /locus_tag="VM406_00175"
     CDS             5636..6136
                     /locus_tag="VM406_00175"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HVL74401.1"
                     /translation="MQEKTFNVLFLCTGNSARSIMAEALISTMGKGRFRGYSAGSKPG
                     GTVNPFAVEQVKSTGYPLENLRSKSWDEFAAPDAPRMDFIITVCDNAAGEVCPVWPGQ
                     PVTAHWGFEDPAAVDGSDETKREAFHKIFRQIAARINSFVNLPVHMLEKNAIKREMDE
                     IGNAAP"
     gene            6139..>6307
                     /locus_tag="VM406_00180"
     CDS             6139..>6307
                     /locus_tag="VM406_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HVL74402.1"
                     /translation="MSTQTRTLGTAIAGASLGFFERYLTIWVAACILAGILLGQAAPG
                     VFQALAALEIAH"
BASE COUNT         1161 a         2063 c         2061 g         1022 t
ORIGIN      
        1 gtatacgacg ccatccgcac cccgcgcggc aaaggcaaga aggacggcag cctgcacacc
       61 gtcaagccgg tgaacctgct gaccggactg atgcatgacc tgcagagccg gcaccgtctc
      121 gataccgctc tggtcgacga cgtagtgctc ggctgcgtga cgccggtcgg cgaccagggg
      181 gcctgcatcg cgaagaccgc cgcgctggcc gccggctggg actggaaggc ttccggcatg
      241 cagctgaacc gtttctgcgc atcaggcctg gaggcggtga acctggccgc gcagaaagta
      301 cgctcgggct gggaagacct ggtggtggcg gggggcgtcg aatcgatgtc gcgcgtgccc
      361 atgggttcgg acggcggcgc ctgggcagcc gaccccgaaa ccagcttcgc caccgacttc
      421 atcccgcaag gcataggcgc ggacctgatc gcgacgctcg aaggcttcag ccgcgacgat
      481 gtcgatgcgt tcgcgatgcg ctcgcagcac aaggcagcgg cggcgcgcgc cgccggccgc
      541 ttccgttcga tgcgcccggt gcgcgaccag aacggcatca cggtgctggc cgaagacgaa
      601 ttcatcaagc cgagcacgac gatggaaacg ctggccggcc tgaaaccctc gttcgaagcc
      661 ccgggcaaga tgggcttcga tgcggtggcg atccgcaagt acccgcaggt cgaacgcatc
      721 cgccacgtcc ataccgcagg caactcgtcc ggcatcgtcg acggcgccgc gctcgcgctg
      781 atcggcagcg agcgcatcgg caagcagctg aacctgaccc cgcgcgcccg catcctcgcc
      841 accgcgatca ccggcaccga cccgaccatc atgctgaccg gccctgcacc ggccagccgc
      901 aaggcgctcg agaaggcggg gctcaccgtc gacgacatcg acctgttcga ggtcaacgaa
      961 gccttcgctg cggtcgtgat gaagttcatg aaggacatgc gcgtgccgga ggagaaggtc
     1021 aacgtcaacg gaggctcgat cgcgctcggc catccgctgg gcgcaaccgg ctgtgcgctg
     1081 ctcggcaccc tgctcgacga actggaggcg cgcaagctga agcggggact ggtcacgctg
     1141 tgcgtgggtg gcggcatggg catcgcgacg atcgtcgaac gcgtctgagg agacaagaat
     1201 gagcatccac tggaacaagg acaccgacgg catcgtcacc ctcacgatcg acatgccggg
     1261 cccggtcaac acgatgaacg ccgccttcca gcaggcgatg ggtgacgccg tcgcccgcct
     1321 cgaggccgag cgcgacgcga tcaagggcgt gatcattgca tccgcgaagg acaccttctt
     1381 cgccggcggc gacctgaagg aattgattgc gattccggcc gacggcgccg cgcagtgctt
     1441 cgagatggtg ggacgcatca aggccgtcct gcgccgtctc gaaaagctcg gccggccggt
     1501 agtcgccgcg ctgaacggca gcgcgctcgg cggcggtctc gagcttgcgc tggcctgcca
     1561 tcaccggatc agcctcgaca acgcgaagat ccagttcggc ttcccggaag tgacgctcgg
     1621 gcttctgccc ggcgcaggcg gcgtggtgcg cagcgtgcgc ctgctcggcc tgcaggaagc
     1681 gatgccctat ctcgtcgagg gcaagcgcat ggcgccggca gaggcgcagg ccaagggctt
     1741 cgtgcacgcg ctggcgaagg accgcgacga tctgattacc caggcgcgcg cctggatcgc
     1801 ggccaaccct gcgcccgtgc agccctggga cgcggacggc tacaagctgc cgggcggcgc
     1861 gccgaacacg ccgaagatcc tgccgctgca gcaggtcgcg cccgcgatgc tgcgcgagaa
     1921 aacccgcggc aactacccgg cgccggaagc catcctgagc gcgatgatcg agggcgcgca
     1981 ggtggatttc gacaacgcgc tggtcatcga aggccgctac ttcacgcaat tagctaccgg
     2041 ccaaatcgcg aagaacatga tcggcacctt cttcttccag atgcaggaag tcggcgccgg
     2101 caaaagccgt ccgaaggacg tgccgaaatg gaaggcgacc aaggtcggca ttcttggcgc
     2161 cggcatgatg ggcgccggca tcgcctgggc caacgccagc cgcggcgtgc cctgcgtgct
     2221 gaaggacgtc gggcgcgaac aggcggagaa aggcaaggcc tacagcgcga agctgctgga
     2281 caagcgcgtc aggcagggcc gcatcagcgc ggacaaggcc tccgctacgc tcaacctgat
     2341 caccccgacc gcggatgcgg ccgatcttgc cggttgcgac ctcgtgatcg aggcggtgta
     2401 cgaaaaccgt gaattgaagg cccaggtcac gcgcgaagcc gccgagcggc tggccgccga
     2461 cggcgtgatg gcgtcgaata cctcgaccct gccgatcacc gggctggccg aagctttccc
     2521 cgaccctgcc cgcttcgtcg gcctgcattt cttctcgccg gtcgacaaga tgcagctggt
     2581 cgagatcatc cgcggcgcga agacatccga gcagacgctc gcacgcgcct acgactatgt
     2641 gcagcagatc ggcaagattc ccatcgtcgt caatgattcg cgcggcttct tcaccagccg
     2701 cgtgttcggg accttcgtca atgaaggcat cgcgatgctt ggcgaaggcg tggagccggc
     2761 gctgatcgag aatgccgcgc tggcggccgg catgccggtc ggaccgctcg cggtccagga
     2821 cgaagtggcg ctgacgctga tgatgcacgt ggcgcggcag acgcgcgcgg acgtgggcga
     2881 cagctatgtg ccgcatccgg ccgaagcgat cgtcgaacgg atggtcgagg aattcaagcg
     2941 ctgcggcaag gccgccggcg ccggcttcta cgaatatccg gccgacggcg gacgcaagtt
     3001 cctgtggccg aagctgcgcg agatcttcgt gaagcccgag gtgcagatct ccttcgaaga
     3061 cgtgcgcgat cgcatgctct acatccagag catcgaaacg atgcgctgcc tcgaagagaa
     3121 ggtgctggaa tcgacgcgcg acgccaatat cggctcgatc ttcggcatcg gctttccagg
     3181 ctggaccggc ggcgccgcgc agttcgtcaa ccacgtgggg gtggaacgct tcattgcgcg
     3241 cgcgactgcg ttgcaggaac gctatggcgc ccgattcgct cccccgcgac tgttgatcga
     3301 actggcggaa tccggccgga cgctgtaagg ggtcccgcgc tgaagtcgcc ccgcctgcca
     3361 tcccgacaaa aacgggttgg cattgatata tgattagcgt cttcagcgcc ttcaacaaaa
     3421 tcaatgccga gcgctcatcg cccgcccctg cgaacctctc acgccatact gccacagttg
     3481 ctggcgggcg ccgccctctt cgccggcgcc gggccggcaa aagcactccc gagtaccatt
     3541 gcgtccaccg tcgccacccc ccacacatgg ctgctgggct tgatggtcgc catgttttct
     3601 gccgccttgc tgctcatcca tcgctaccgg cgcgcggttc taagggcgcg catggcggaa
     3661 gacgaactgc gtggggtacg tgccgccaac gccgagctgc gtctgcagct agccgcgctg
     3721 cgcgaacaag gcgaagccag caaggtcgac gcggaacgct ttcgcagcct catcgcgatt
     3781 tcatctgatt ggtactggga acaggatgct gacctgcggt tcacgatgcg cacgggctgg
     3841 tcactcggcg aggaggtgct ggtgcgtcac caatacctgg gattgcgccg ctgggagcag
     3901 cccggcattg ccggccccgt cgactgggat gcgcatcgtc gcgacctgga cgcgcgccgg
     3961 ccgttccggg ggctggaata ccggctacag atcggcaacg aagtcgtctg ggtcagcatc
     4021 aatggcgaac cgatattcgg ttccgacggc accttcctcg gctatcgcgg cacggggcgc
     4081 gacatttcac agaggaagcg gatcgaggcc gaactgcgcg ccagcgaaga gcgcttccac
     4141 gaaatcgtcg gcagcatgcc ggtgggcctg ttcatcaagg accccgaggg ccgcgtcatc
     4201 ctgatgaacc gcgaatgcga gcgccagtgg ggcgtgcgct tcgagcagat ccgcaatacc
     4261 gacggctccg cgttctatcc gcgcgagacg atgaacgcgt tccgcgcaca ggatcggcac
     4321 gccttcgcct gccgccgggt catcgagtac gaggaattgg tctcgccgct cgatcactcg
     4381 cgccccatca tcgcgcgcgg catgaagaaa ccggtgtatg acgaggccgg gcaaccgcgc
     4441 tatctgatcg gcatctcgat cgacatcagc gcacacaagc gttccgagga acagctgcgc
     4501 gaatcgcgcg aggcgctgcg ccagcttgcg cgttaccaga cgcatgtgaa ggaagaggag
     4561 cgcaagcgca tcgcgcgtga tatccatgac gaactcggcc agaacctgat ggcgcttcgc
     4621 ctcgatgtcg gatcgctgat caacggcgtg tccggcgatg gttcggcact gtccgagcgg
     4681 gcgaagctga tgatggagac cgtggaccgc accatacgca gcgtgcgctt gatcatcaac
     4741 gacctgcgtc ccgcggtgct ggaccttggg ctgcaggcgg ccgtcgaatg gcaggtagcc
     4801 gagttcatgc gccgcaccaa catttcctgc acgctcgaaa tcgacttcgg tgagggcgag
     4861 ccggcggtcg aagacgacgt agccaccacc ttcttccgcg tgctgcagga gtcgctgacc
     4921 aacgtgcagc gccatgccaa tgcgtcctgg gtgcgggtcg gcctgcatca ggacggaacc
     4981 tgcctgcggc tctccattgc cgatgacggc aagggaggcg cgctgatccg ccgcggcaag
     5041 cacagcttcg gcctgctggg aatgcgcgag cggctcaagg ctctcggcgg ctcgctggaa
     5101 gtcgtcagca agactggcgt cggcacccgc ctcaccgcct cgctgccgct taaagccgcc
     5161 cccctggccc agtcctagcc ctccctggcc cgccgggcgc acattcctgc cacgctgcta
     5221 ttgccggctt tgccttgttc tatatttcga ttattattga accgttgaag tgaaggagat
     5281 gtcatgcaga ccagggacgt cattgccgcg cttgccgcgc tcgcccagga atcccggctt
     5341 gcggttttcc gtttgctggt ccaggcaggg ccgtccggga tggccgccag ccgaattgct
     5401 gaacaggtgg ggattgcccc ctcctcgctc tcgtttcatc tgaaggagct gacccatgcg
     5461 ggactggtta cgccttcacc ggaaggccga ttcgtcatct atcgcgcgaa ctttgacacg
     5521 atgaatggcg tgctcgcatt cctgacggac aactgttgcg gcggcaatcc ctgcgctccg
     5581 gttgccgcct gtgtaccgga acaacccatt gagttcaaga aaagagggag taaacatgca
     5641 agagaagacg tttaacgtgc tgttcctgtg caccggcaac tcggcgcgca gcatcatggc
     5701 cgaggcgttg atcagcacga tgggcaaggg ccgcttccgc ggctattcgg ctggcagcaa
     5761 accgggggga acggtgaacc cgtttgccgt cgagcaggtg aagtccaccg gctatccgct
     5821 cgagaacctg cgcagcaaga gctgggacga attcgcggcg ccggacgcgc cgcggatgga
     5881 tttcatcatc accgtctgcg acaacgccgc cggcgaggtc tgcccggtct ggcccggaca
     5941 gccggtgacc gcgcattggg gcttcgagga tccggcggca gtcgacggca gcgacgagac
     6001 aaagcgcgaa gccttccaca agatctttcg gcaaatcgcc gcgcgcatca acagtttcgt
     6061 gaatcttccg gtgcacatgc tcgagaagaa cgcgatcaag cgcgaaatgg acgagatcgg
     6121 caacgccgcg ccatgaacat gtcgacgcaa acccgcaccc tgggcaccgc gattgccggc
     6181 gcctcgctcg gcttcttcga gcgttacctg accatctggg ttgctgcctg catcctcgcc
     6241 ggcatcctgc tcggccaggc ggcgccgggc gtgtttcagg cgctggcagc gctcgagatc
     6301 gcccacg
//
LOCUS       DATLGF010000007         4926 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_79466,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000007 DATLGF010000000
VERSION     DATLGF010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79466"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VM406_00185"
     CDS             <1..223
                     /locus_tag="VM406_00185"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HVL74403.1"
                     /translation="ATPIVLSDRGDAQFPHHEVMVNLSPATPALFARFERLIELVGLD
                     EADRAAGRERFRHYRQRGYPIEHRNAGQK"
     gene            220..594
                     /locus_tag="VM406_00190"
     CDS             220..594
                     /locus_tag="VM406_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74404.1"
                     /translation="MSSFRPDAGIPVLTEIIDTPEQLDEPAPATPLASPAAVPAASDP
                     LDLERWEQLERELHERILQQVLKHIDFALEQRVRDSLADVLQTAVEQLAGDIRSGLQQ
                     TVKDVVTRAVMHEINVARTSKK"
     gene            733..2049
                     /locus_tag="VM406_00195"
     CDS             733..2049
                     /locus_tag="VM406_00195"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HVL74405.1"
                     /translation="MKVIVLGAGIIGTASAWFLHRAGHEVTVLEREPGAALETSFANG
                     GQISVSHAEPWANPSAPLKVLKWLGQEDAPLLFRLRPEWLQWRWGMQFLRECTPARTA
                     HNIRQIVAIAEYSRQTLDAVRAETGIEYDALTRGILHFYTDAKEFEHSLPAARLMREL
                     GCPRESISADEVVKIEPSLASIRHKIVGGDFTPTDESGDVYKFTAGLADKAAAGGVDF
                     RYNTTITRLLTEGTGAAARITGIEIIDAHGRHQVVRADAFVVALGSFSQPLLKPLGID
                     LMIYPGKGYSATYTITNPDTAPSVSLTDDGYKLVFSRLGDRLRVAGTCELNGYSRELN
                     STRCEAITRRTRELFPDACNYDDPVYWTGLRPLTPSNVPYIGKTRFANLFLNTGHGTL
                     GWTMGCGSGRAIAEIVSGRIPDVDFAFTGMSRTAMPARAATAAARA"
     gene            complement(2084..2593)
                     /locus_tag="VM406_00200"
     CDS             complement(2084..2593)
                     /locus_tag="VM406_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HVL74406.1"
                     /translation="MPKTQVLDKVDRKLLNLLQKDNQLPTRVLAEKVHISQPTCLRRV
                     RELRETGIIGADVSLVDPFALGYGMLAFLEVSLNNQSDEHMQDFEARMGKEPEVMQCY
                     FVSGDYDYFLVVHVVDMDAYYQFVRRVISGSGNVRHFQSRFPMKRAKFVTRIAFDEKA
                     PEVQVKVGR"
     gene            2820..3965
                     /locus_tag="VM406_00205"
     CDS             2820..3965
                     /locus_tag="VM406_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HVL74407.1"
                     /translation="MQFKTKMIPLAGAIAFMVAGTAAAQEQVVRIGHVGPISGSIAHL
                     GKDNENGARMAIEELNAKGITIGGRKIKFELLAEDDAADPKQGTAAAQKLVDAKVHGV
                     IGHLNSGTTIPASKIYNDAGIVQISPSATNPRYTQQGYAGAFRVVANDGQLGGTLGRY
                     AADQLKAKRVAVIDDRTAYGQGVAQEFVKGARAKGVEVVAQQYTNDKATDFNAILTAI
                     KARNPDVVFFGGMDAVAGPMLRQMKALGINAKFMGGDGICSQTLANLAGDAVGENQVY
                     CAEAGGVAETEKKGMEEFKARYKQRFGTDVQIYAPYVYDSVMIMVQAMQRANSWESDK
                     YLPELKKTNYKGVTGQIAFDDKGDIKDGALTLYTYRGGKRTELAVVK"
     gene            complement(4299..4667)
                     /locus_tag="VM406_00210"
     CDS             complement(4299..4667)
                     /locus_tag="VM406_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HVL74408.1"
                     /translation="MDRIFFAIGALSALISVAAGAFGAHGLRTRLGPDMLNVFEIGVR
                     YQMYHALALLAVAWACTRWPHPATTASGVLFIVGTVVFSGSLYVLSLSGIRWLGAVTP
                     FGGVALIAGWGCLAWAAARG"
BASE COUNT          979 a         1610 c         1536 g          801 t
ORIGIN      
        1 cgccacgcct atcgtcctca gcgatcgcgg cgacgcgcag tttccccacc atgaagtgat
       61 ggtgaacctc tctccagcga cgccggcgct gtttgcccgc ttcgagcggc tgatcgaact
      121 ggtcggcctg gacgaggccg accgcgccgc cggacgcgag cgctttcgcc actaccggca
      181 acgcggctat ccgatcgaac atcgcaacgc aggacagaaa tgagcagctt caggcccgat
      241 gccggcattc ccgtgctcac cgagatcatc gacaccccgg agcagctcga tgaacccgct
      301 ccggcaactc cgctcgcctc tcccgctgcc gtgccggcgg catccgatcc gctcgacctc
      361 gaacgctggg aacagctcga acgcgagctg cacgaacgca tcctgcagca ggtgctgaag
      421 cacattgact tcgcgctcga acaacgggtg cgcgacagcc tggctgacgt cctgcagacc
      481 gcggtcgagc agctggccgg cgacatccgc agcggtttgc agcagaccgt gaaggatgtg
      541 gtgacacgtg cggtgatgca cgagatcaac gtcgcgcgca cttcgaaaaa ataaaaaatc
      601 cccgaggatt tcgtggcttc gcgaacatgc gccggccacg cgtgccgcag gcggaaaatt
      661 tttacagccc tgaatgatca ctcaggaaaa cgataagtcc atacaatctc gacatcgttt
      721 ttggagtcaa gcatgaaagt gatcgttttg ggcgccggca tcatcggcac cgcatcagcg
      781 tggttcctgc acagggccgg gcatgaagtc acggtgctcg agcgcgagcc gggcgccgcg
      841 ctggagacca gtttcgccaa cgggggccag atctcggtgt cccacgccga accctgggcc
      901 aatccgtcgg ccccgctgaa ggtgctgaag tggctggggc aggaagatgc gccgctgctg
      961 ttccggctgc gtccggaatg gctgcaatgg cggtggggca tgcagttcct gcgcgaatgc
     1021 acgccagcgc gcaccgcgca caacatccgc cagatcgttg ccatcgccga atacagccgg
     1081 caaacgctcg acgccgtgcg cgccgagacc ggcatcgaat acgacgccct cacccgcggc
     1141 attctccact tctataccga cgcgaaggaa ttcgagcact ctcttcccgc cgcgcgcctg
     1201 atgcgagagc tgggctgccc gcgcgaatca atcagcgccg atgaagtcgt gaagatcgaa
     1261 ccgtcgctgg cctcgatccg gcacaagatc gtcggcggcg atttcactcc gaccgatgaa
     1321 tccggcgacg tgtacaagtt caccgccggg ctcgcggata aagccgccgc cggtggcgtg
     1381 gatttccgct acaacaccac gatcacccgc ctgctcaccg agggaaccgg cgcggcggcc
     1441 aggatcaccg gcatcgaaat catcgacgca cacggccggc accaggtggt gcgcgccgac
     1501 gccttcgtgg tcgccctggg cagcttttcg cagcctttgc tcaagccgct gggcatcgac
     1561 ctgatgatct atcccggcaa gggctactcc gccacctaca cgatcacgaa tccggacacc
     1621 gccccttcgg tttcgctcac cgacgacggc tacaagctgg tgttctcgcg cctgggcgac
     1681 cgcctgcgtg tggccggcac ctgcgagctc aacggctatt cgcgcgaact caacagcacg
     1741 cgctgcgaag cgatcacgcg acgcacgcgc gaactgttcc cggatgcctg caactacgac
     1801 gatccggtct actggaccgg actgcggccg ctgacgccgt cgaacgtgcc ctatatcggc
     1861 aagacccggt tcgcgaacct gttcctcaac accgggcatg gcacgctcgg gtggacgatg
     1921 ggctgcggct cgggtcgtgc aatcgcggaa atcgtttccg ggcgcattcc ggacgtcgac
     1981 ttcgcgttca ccggcatgtc gcgcacggcg atgccggccc gcgctgcgac cgcagccgca
     2041 cgggcctgaa gaatcgctcc cgcgctcgcg ggagccccct cccctaccgc ccgaccttca
     2101 cctgcacctc gggcgccttc tcgtcgaacg cgatgcgcgt gacgaacttc gcccgtttca
     2161 tcgggaagcg cgactggaag tggcgcacat tgccggagcc ggagatcacc cgccgcacga
     2221 actggtagta cgcgtccata tcgaccacgt gcacgacgag gaagtagtcg taatcgcccg
     2281 acacgaaata gcactgcatc acctcgggct ccttgcccat gcgcgcctcg aaatcctgca
     2341 tgtgttcgtc ggactggttg ttcagcgaca cctcgaggaa ggccagcatc ccgtagccca
     2401 gcgcgaacgg atcgaccagc gacacgtccg cgccgatgat cccggtctcc ctcagttcgc
     2461 gcacgcggcg caggcaggtc ggctgcgaga tgtgcacctt ctccgccagc acccgcgtcg
     2521 gcagctgatt gtccttttga agcagattca acagcttgcg atcgaccttg tcgaggacct
     2581 gagttttggg catataaaaa tcgctgaaat tcgaaaaaaa ccggagaaaa gttctaggcc
     2641 gggcgtttct tctgcgttac cgtctggctg gtcggaaaag aacggtatca aagtggcacg
     2701 gcatgcggaa caggccggga cagtgtcgat gccgatcgaa cctgcacaag gccgacgcgc
     2761 aaaaacgaaa aagtgttgca gcaaccctgt gtcgtttcaa tccttaggga ggatatttta
     2821 tgcagttcaa gacaaaaatg attccgctgg cgggcgcgat tgcgttcatg gtcgccggca
     2881 cggccgcggc gcaggagcag gtggtgcgca tcggccacgt tggtccgatc tccggctcga
     2941 tcgcccacct gggcaaggac aacgagaacg gcgcgcgcat ggcgatcgag gagctcaatg
     3001 cgaagggcat cacgatcggc ggccggaaga tcaagttcga actgctggcg gaagatgacg
     3061 ccgccgaccc gaaacaaggc accgcggccg cgcagaagct ggtcgatgcc aaggtccacg
     3121 gcgtgatcgg ccacctgaac tcgggcacca cgattccggc ctcgaagatc tacaacgatg
     3181 ccggcatcgt gcagatctcg ccgtcggcga ccaacccgcg ctacacccag cagggctatg
     3241 ccggcgcgtt tcgcgtggtg gccaacgacg gccagctcgg cggcacgctg ggccgttacg
     3301 cggccgacca gctgaaggcc aagcgcgtgg cggtgatcga cgaccgcacc gcgtacggcc
     3361 agggcgtggc gcaggagttc gtcaagggcg cgcgcgccaa gggcgtcgaa gtggtggcgc
     3421 agcaatacac gaatgacaag gcgaccgact tcaacgcgat cctgaccgcg atcaaggcgc
     3481 gcaatccgga cgtcgtgttc ttcggcggca tggatgcggt ggccggcccg atgctgcgcc
     3541 agatgaaggc gctgggcatc aacgccaagt tcatgggcgg cgacggcatc tgctcgcaga
     3601 cgctggccaa cctggccggc gacgcggtcg gcgagaacca ggtgtactgc gccgaagccg
     3661 gcggcgtggc cgagaccgag aagaagggca tggaggaatt caaggcgcgc tacaagcagc
     3721 gcttcggcac cgacgtgcag atctatgcac cgtacgtgta cgactcggtg atgatcatgg
     3781 tgcaggcgat gcagcgcgcc aactcctggg agtcggacaa gtacctgccg gagctgaaga
     3841 agaccaacta caagggcgtg accggccaga tcgcgttcga cgacaagggc gacatcaagg
     3901 atggcgcgct gacgctgtac acctaccgcg gcggcaagcg caccgagctg gcggtggtca
     3961 agtaatcggc ttcgctgcgt tcacgaacag ccccggcttg ccggggctgt tttttttggg
     4021 gttgcgtcct cgcttgccgg agggatgaga tccctcactg cgttcgggag gacagtaatc
     4081 aggcgatgcc cctggatctg tcgttccgag cgcagcaagg aacctcatcc gctcgctttg
     4141 cgccaccgct tgccgggcgg atgaggtccc tcactgcgtt cgggacgaca gtatttgggg
     4201 atgacagttt tttgggatga cagaaatcag gcgatgtccg ctggacctgt cgttccgagc
     4261 gcagcgagga acctcatccg ctcagtacga cgatgacttc acccccgcgc cgccgcccat
     4321 gccaaacacc cccagccggc gatcagcgcc acgccgccga acggggtgac tgcacccagc
     4381 cagcgtatgc cggacaggct cagcacataa agcgatcccg agaacaccac tgttcccacg
     4441 atgaacaaca cgccgcttgc ggtcgtggcc ggatgcggcc agcgcgtgca ggcccacgcg
     4501 acggccagca gcgccagcgc atggtacatc tggtagcgca cgccgatttc gaatacgttg
     4561 agcatgtccg gaccgagccg ggtgcggagg ccgtgcgcgc cgaaggcgcc ggcggcgacg
     4621 ctgatcaatg cggaaagcgc gccgattgcg aagaagatgc ggtccatgtc ggatagcgga
     4681 tagccgtggg aaaggggatg tcattgtgcc gcgaatcgcc agccgctgcc gatgcatgat
     4741 cggcatacaa tgccagttcc tctgactgcc gcacgcatcc atgcccgccc gcgacaaaca
     4801 tatcgccctc atctataccg gcggaaccat cggcatgacc cggactgcca atggttacgc
     4861 gccgatggcc gacttccccg accgcctcga cgcattgctg gccggcgccg gcgacgcgat
     4921 gccacg
//
LOCUS       DATLGF010000008        10792 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_16307,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000008 DATLGF010000000
VERSION     DATLGF010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 10792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10792
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16307"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VM406_00215"
     CDS             <1..359
                     /locus_tag="VM406_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL74409.1"
                     /translation="MPRVLALAIAMPLLVIWTDAVALVGGIAAAQFELGLTFAYFMQE
                     LPNAVPIANFWIGLGKGVVFGILIALVACHFGLRIEANTESLGQGTTLSVVTAITAVI
                     IADAIFAVVFADVGFY"
     gene            359..1147
                     /locus_tag="VM406_00220"
     CDS             359..1147
                     /locus_tag="VM406_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVL74410.1"
                     /translation="MAPVIEIHGLRTAFGAHVVHEDLDLSVEAGDILALVGGSGSGKT
                     TLLRHMLGLTRPQRGSVHVFGEEIGRLNSRRLQQVRNRWGMLFQHGALFSALTALENV
                     MLPLQELRTLPAPLVREAALLKLAMVGISASDAQKMPADLSGGMVKRVALARALALEP
                     ELLFLDEPTAGLDPSLSDSFVALIRSLHEHMRLTVVMVTHDLDTLFALATKVAVLADR
                     RVIACDAPHRVLEVEHPFITHFFLGERGKRALQEVRDTESEHGK"
     gene            1137..2072
                     /locus_tag="VM406_00225"
     CDS             1137..2072
                     /locus_tag="VM406_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HVL74411.1"
                     /translation="MENKSHAIAAGLFTILLVAATIAIGMWLTRDQSTWIPYEIATEH
                     SIPGLSPQAAVRYRGLDVGRVDTIRFDPEQAGRILVGIRVRPDTPITHSTFASLGYQG
                     VTGVAYVQLDDDGSKPAPLPSSEADPARLAMRPGLFEQLQYRGLAIMEQTEDLARRLN
                     VLMTDENQQTILAAFDNVSRAAREIEKVPRQLEPALASIAPVMRRAERAMGSLETVAG
                     NGARLTANLDGMVDDLRAPDGTLARIAGAAEQVGSVAYRIENETLPIVTDVRSSIRAL
                     NRALDNLAERPQSVLYGAPVVAPGPGEPGFVAPSR"
     gene            2081..2701
                     /locus_tag="VM406_00230"
     CDS             2081..2701
                     /locus_tag="VM406_00230"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HVL74412.1"
                     /translation="MRIPQCSTRLIAMLLALPLALSGCAAIRSEAPMMFDLGPVRLAP
                     DTRLLAGTEPVNVAEVATPAWLDSQYIYFRLNYAEPQQPRPYVASRWVMPPAQMLAQQ
                     IRQRIAQAGGIALRPGDGAFDAPTLRVQVDDFSQQFAGPERSAGRVALRATLLRGRTV
                     LGQRTFIHEVPAQRADAAGGAHALAVASDAALGELVAWLATLELRR"
     gene            2766..3926
                     /locus_tag="VM406_00235"
     CDS             2766..3926
                     /locus_tag="VM406_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HVL74413.1"
                     /translation="MNISDTPSFSASETLTAHEAGQVSTFARAGLLMYTCLIVYASLY
                     PFSNWHRMGLKAWDFLFMPMPYYWTGFDLATNVVGYVPFGILVVFALYPRVRGVAAAV
                     TATFLAMVLTGSMEALQTFLPNRVSSNLDFLANLGGGAIGAIAGALLTRSFLVESRML
                     QLRREWLVPEAGRGAIVAGLWMLAQIYPQPYMFGHGQVFPILSEWISRLTEAPIDLGA
                     LLRGDATLSIQDYWLAETLITASGMSGALLAWSFLLRKQAPTGLLIAGLLAGAALVKF
                     MSYALLFTPALALDWLTPGAKGGMLLGVLLVAGVAYAPPTVQRRIAIACLLISLVLIN
                     AMPENPYFAETLQSWIQGKFLNFNGAAHFLSLFWPFLALWFLLHPAHRKKRN"
     gene            3961..4281
                     /locus_tag="VM406_00240"
     CDS             3961..4281
                     /locus_tag="VM406_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HVL74414.1"
                     /translation="MNDKPYYEHHVFFCLNERPEGERTCCAAKGAQAAQEHAKKRIKE
                     LKLNGPGKVRINKAGCLDRCEEGPVVVIYPQGTWYTYVDKDDIDEIIDEHIVNGRVVE
                     RLKI"
     gene            4377..5003
                     /locus_tag="VM406_00245"
     CDS             4377..5003
                     /locus_tag="VM406_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL74415.1"
                     /translation="MNAQTQRFTLQGPAGALECALDLPADGAPRGIALVAHPHPLYGG
                     TMDNKVAQTLARAFVALGYAAVRMNFRGVGQSEGVHDEGRGETDDMALLLDHMQERYP
                     GLPVALSGFSFGTFVQSQLQQRLIAAGRPAERLVLVGSAAGKWAMPDIPANTILIHGE
                     YDETIPLSAVLNWARPQDIPVIVIPGADHFFHRKLQHIKNFVVEMWHR"
     gene            5151..6296
                     /locus_tag="VM406_00250"
     CDS             5151..6296
                     /locus_tag="VM406_00250"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HVL74416.1"
                     /translation="MKKLIAAIAATAFSVSAAWAQALPAPTIAAKSWLLLDATSGQVL
                     ASHEPTMRIEPASLTKIMTAYLVFSAIKEKRLDPEQLITVSVNAWKVDPGSSKMFIEP
                     RVPVSINDLLYGLIVQSGNDAAIALAEAVAGTEEAFVQLMNREAERMGLKSTRFSNPH
                     GLTSNDTYSTAQDLATLAHRLINDHPDFFRIYSTREFTYNKIKQQNRNRLLWLDPTVD
                     GMKTGYTAAAGFCLISTARRPNGDHERRLISVVLGTASDALRAQESQKLLNWGFQNFD
                     TVRLFAKGEPVATPQVWKGSLNKVKVGFTQDIFVTVPKGQGERIKPVLERTDPLIAPV
                     AQYSNIGTLKMMVDGKPIAELPVVSLEQVNQATVFGRAWDSVRLWIK"
     gene            6414..7256
                     /locus_tag="VM406_00255"
     CDS             6414..7256
                     /locus_tag="VM406_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HVL74417.1"
                     /translation="MQDPLVYLNGELTPLSQARIPVLDRGFIFGDGVYEVMPLYRRKP
                     FRAKQHMARLFRSLSEIGIANPHTEDDWMGLISSVADAFPADDQLVYIQVTRGVAKRA
                     HAFPKDVQPTVFIMTNPLVLPTDAQRADGLACVTFEDRRWLMCHIKSISLLGNVLAAQ
                     HAAEQGAAEVIQFRDGFLSEGAASNVWIVKDGTLMAPPKDNLILEGIRYGLLEELCEA
                     TGTPFEARRISREEVFAADEVLMSSASKEVIAVTSIDGKPVADGKPGPVYSRLLAAYR
                     EKTL"
     gene            7408..7677
                     /locus_tag="VM406_00260"
     CDS             7408..7677
                     /locus_tag="VM406_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HVL74418.1"
                     /translation="MTDQRDSLIEYPSDFPIKIMGVMQDEFAQTMVDVVVRHDPTFHA
                     GRMEMRASSKGTYLSLTVTVLATSREQLDNLYRELSSHPMVKVVL"
     gene            complement(7899..9251)
                     /gene="gorA"
                     /locus_tag="VM406_00265"
     CDS             complement(7899..9251)
                     /gene="gorA"
                     /locus_tag="VM406_00265"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HVL74419.1"
                     /translation="MTNTRFDVDLFTIGGGSGGVRASRFAAQYGARVALAERHKLGGT
                     CVNAGCIPKKLMSYAAHYRDDLAHAPAYGWDVGPAAFDWKTLLRNKDAEISRLNGVYR
                     SLLEKSGVTVHEGNAVIEDPHTVNIDGKRVTARHILVATGGRPSVPEITGKALGIVSD
                     DFFHLDDVPKRTVIAGGGYIAVELAAILNGLGSQVTLVHRGARLLRAMDADIGSFLGK
                     EMEKKGIAIKGGTVIASLASDGQGIRRALLDNGETLDAEAVLFATGRVPNTEGLGLEN
                     VGVRLSERGAIKVDANFTTSVPSIHAVGDVIDRVALTPVALAEGMVVASLLFCDGARK
                     LSYENIPTAVFSHPNVGTVGLTEATARERHAKVRVFKSEFTALKHTISGASERVMMKL
                     VVDEASDRVLGVHMVGADAGEIIQGFAVALQCGATKAQFDATIGIHPTIAEEFVTMRG
                     "
     gene            complement(9362..10255)
                     /gene="gcvA"
                     /locus_tag="VM406_00270"
     CDS             complement(9362..10255)
                     /gene="gcvA"
                     /locus_tag="VM406_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HVL74420.1"
                     /translation="MSDFRRLPNLAALRAFEAAARHGNFSRAAEEVHVTHGAISHQVR
                     ALEEELGVALFARHGKRITVTPEGEQFAAVVRRALGDVAAAAEALKITARQQRLTITA
                     LPSLASRWLAPRLGAFIDRHPDLEVMLQSSHALTDFVRESVDVGLRFGLGKYPGLNVE
                     KLMDDAFYPVASPRFNGGRLPKTPQQLKHMPLLRCELEPWTPWFRAAGLDLLEPTGGL
                     VFQDSSQLVRAAVEGNGIALARHVLAVGEIESGQLVRLFDVAIPCPQSYFFVCPPEAL
                     VKPQVQAFREWVMGEAQGMVC"
     gene            10400..10753
                     /locus_tag="VM406_00275"
     CDS             10400..10753
                     /locus_tag="VM406_00275"
                     /inference="COORDINATES: protein motif:HMM:NF022592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HVL74421.1"
                     /translation="MRDLFTSEAIHLEAGEVLSGAAKRARILRIKSGSAWVTVEGNRY
                     DYWLSAGDSLRVATGRLVVVEAQTGGFEAQTRVETAPAPLATEGMLRALLGTLRRWLR
                     QDRVTAPTVERRCCA"
BASE COUNT         1912 a         3629 c         3378 g         1873 t
ORIGIN      
        1 tcatgccgcg cgtgctggcg ctggcgattg ccatgccgct cctggtgatc tggaccgatg
       61 cggtcgctct cgtcggcggc atcgcggccg cccagttcga acttggattg acatttgcat
      121 acttcatgca ggaattgccg aacgccgtgc cgatcgcgaa tttctggata ggactcggca
      181 agggcgtcgt gttcggcatc ctgatcgcgc tggtggcctg ccatttcggc ctgcgcatcg
      241 aagccaacac cgaaagcctg ggacagggca cgacgctgtc ggtcgtgacc gcgatcaccg
      301 ccgtaatcat cgccgacgcc atcttcgcgg tggtgtttgc cgacgtgggg ttctactgat
      361 ggcgccggtg atcgagatac acggactgcg cacggcgttc ggggcgcacg tcgtgcacga
      421 ggatctggac ctcagcgtcg aagccggcga cattcttgcg ctggtcggcg gatccggctc
      481 cggcaagacc acgctgctgc ggcacatgct cggcctgacg aggccgcagc gcggcagcgt
      541 gcacgtattc ggcgaggaaa tcggccgact caacagcagg cgcctgcagc aggtgcgcaa
      601 ccgctggggc atgctgttcc agcacggcgc gctgttctcg gcgttgacgg cgctcgagaa
      661 cgtgatgctg ccgctccagg aactgcgcac cctgccggcg ccgctggtgc gcgaagcggc
      721 actgctgaag ctcgcgatgg tcggcatcag cgcgagcgac gcgcagaaga tgccggccga
      781 tctgtccggc ggcatggtca agcgcgttgc actggcgcgg gcgctggcgc tggagccgga
      841 actgctgttc ctggatgaac cgacggccgg gctggatccg agcctgtcgg acagtttcgt
      901 cgccttgatc cgcagcctgc acgagcacat gcggctgacg gtggtgatgg tcacgcacga
      961 tctggacacg ctctttgcgc tggccacgaa ggtggcggtg ctggccgacc ggcgcgtgat
     1021 cgcctgcgac gcgccacacc gggtgctgga agtcgagcac ccgttcatta cccatttctt
     1081 cctcggcgag cgcggcaagc gcgcgctgca ggaagtgcgc gacacggagt ctgaacatgg
     1141 aaaataaatc ccacgccatc gcggcagggc tgtttacgat actgctcgtg gccgccacca
     1201 tcgccatcgg catgtggctc acgcgcgacc agtcgacctg gatcccgtat gaaattgcaa
     1261 ccgagcactc gatccccgga ctcagtccgc aagccgccgt acgctatcgc gggctggacg
     1321 tcggccgggt cgacacgatc cgtttcgatc cggagcaagc cggacgcatc ctggtcggca
     1381 tccgcgtacg cccggacacg ccgatcacgc actccacctt cgcttcgctc ggctatcagg
     1441 gcgtgaccgg cgtggcctac gtgcagctcg acgacgacgg cagcaagccg gcgccgctgc
     1501 ccagcagcga agccgatccg gcgcgcctgg cgatgcgtcc cgggttgttc gagcaattgc
     1561 agtaccgcgg tctggcgatc atggagcaga ccgaagatct cgcgcgccgc ctgaacgtgc
     1621 tgatgaccga cgaaaaccag cagaccatac ttgccgcctt cgacaacgtc agccgcgccg
     1681 cgcgcgaaat cgagaaggtg ccgcgccagc tggaaccggc cctggcgagc attgcgccgg
     1741 tgatgcggcg cgcggaacgg gcgatgggat cgctggagac cgtggccggc aacggtgccc
     1801 ggctgaccgc caacctcgac ggcatggtcg acgacctgcg cgcacccgac ggcacgctgg
     1861 cgcgcatcgc cggcgccgcc gagcaggtcg gttcagtggc atatcgcatc gagaatgaaa
     1921 cgctgcccat cgtcaccgat gtgcgctcgt cgatacgcgc gctcaaccgc gcgctggaca
     1981 atctcgccga acgcccgcag agcgtgctgt acggcgcacc ggtggtggcg cctggtccgg
     2041 gcgagcccgg tttcgtcgcg ccgtcgcgct aggagattcc atgcgcatcc cccaatgttc
     2101 gacacgcctg atcgcaatgc tgctcgcgct gccgctcgcg ctgtccggct gcgccgcgat
     2161 ccgttcggaa gccccgatga tgttcgatct cggcccggtc aggctggcgc cggacacccg
     2221 gctgctggcc ggcacggagc cggtcaatgt ggcggaggtg gcaaccccgg catggctgga
     2281 cagccagtac atctacttcc ggctcaacta cgccgagccg cagcagccgc gcccgtatgt
     2341 ggccagccgc tgggtgatgc cgcctgcgca aatgcttgcg caacagatcc gccagcgcat
     2401 cgcccaggca ggcggcatcg cactgcgccc gggcgacggc gccttcgatg cccccacgct
     2461 gcgcgtgcag gtcgatgact tcagccagca gtttgccggc cccgagcgca gcgccggccg
     2521 ggtcgcgctg cgcgccacgc tgctgcgcgg gcgcaccgtg cttggccagc gcaccttcat
     2581 ccacgaagtg ccggcgcagc gcgcggacgc cgccggtggc gcccacgcgc tggccgtcgc
     2641 cagcgacgct gcgctgggcg agctggttgc atggctggca acgctggagc tgcgccgctg
     2701 atttcgcttg cccgccgtgc gcgcactacc gacaatagtg cgcatttgcg cgccacgccg
     2761 accgagtgaa catcagcgac accccatcct tttctgcaag cgagaccctg accgcccacg
     2821 aggccggaca ggtgtcgact ttcgcgcggg cgggcctgct gatgtacacc tgcctgatcg
     2881 tctacgcaag cctgtatcca ttttcgaact ggcatcgcat gggcctgaag gcatgggact
     2941 tcctgttcat gccgatgccc tactactgga ccggcttcga tctggcgacc aacgtggtcg
     3001 gctacgtccc cttcggcatc ctggtcgtat tcgcgctgta tccgcgcgtg cgcggcgtag
     3061 cagctgccgt gacggccacc ttcctcgcga tggtgctcac tggaagcatg gaagcgctgc
     3121 agaccttcct gcccaatcgc gtctcctcca atctcgactt tctcgccaac cttggcggcg
     3181 gtgcgatcgg cgcgatcgcc ggcgcgctgc tcacgcgcag cttccttgtc gaaagccgga
     3241 tgctgcagct gcgccgcgag tggctggtgc ctgaggccgg gcgcggcgcg atcgtggccg
     3301 gcctgtggat gcttgcgcag atctaccccc agccgtacat gttcggccac ggccaggtgt
     3361 ttccgatcct gtcggagtgg atatcccgat tgaccgaagc gccgatcgat ctcggtgcgc
     3421 tgctgcgcgg cgacgccacg ctgagtatcc aggactactg gctcgcggag accctgatca
     3481 ccgccagcgg aatgagcggc gcgctgctcg catggtcctt cctgctgcgc aagcaggcgc
     3541 cgaccgggct cttgatcgcc ggcctgctcg cgggcgcggc gctggtcaag ttcatgtcgt
     3601 atgcgctgct gtttacaccg gcgctggcgc tggactggct cacgcccggc gccaagggcg
     3661 gcatgctgct gggcgtgctg ctggtggcag gcgtggccta tgcgccgccg accgtgcagc
     3721 ggcgcatcgc gatcgcctgc ctgctgatca gcctcgtgct catcaatgcg atgcccgaga
     3781 atccgtactt cgcggagacg ctgcagtcgt ggatacaggg caagttcctc aacttcaacg
     3841 gcgccgccca tttcctctcg ctgttctggc ccttccttgc gctctggttc ctgctgcacc
     3901 cggcgcaccg gaaaaaacgc aactgatatc atggcgcgtt gacgtatcga gcgccgtgac
     3961 atgaacgaca agccgtatta cgaacaccac gtcttcttct gcctgaacga acggcccgaa
     4021 ggcgagcgca cctgctgcgc cgccaagggt gcgcaggcgg cccaggaaca cgcgaagaag
     4081 cgcatcaagg aactcaagct caacggcccg ggcaaggtgc gcatcaacaa ggccggctgc
     4141 ctcgaccgct gcgaggaagg cccggtggtg gtcatctacc cgcaaggaac ctggtacacc
     4201 tacgtggaca aggacgacat cgacgagatc atcgatgagc acatcgttaa cgggcgcgtg
     4261 gtggagaggt tgaagatctg atcatgtgag gacctccgcg caacgcgcgc agagacgccc
     4321 tcccctgctt cgggcggagg caacgcgtcc acgcaattga aaacaacaag ccatccatga
     4381 acgcccaaac ccaacgcttc accctccaag gtccggccgg cgccctcgaa tgcgcgctcg
     4441 acctccccgc cgatggcgcg ccgcgcggca tcgccctggt cgcccacccg cacccgctgt
     4501 acggcggcac aatggacaac aaggtcgccc agaccctcgc gcgcgccttc gttgcgctcg
     4561 gctacgcggc cgtgcgcatg aatttccgtg gggtcggcca gtccgaaggc gtgcatgacg
     4621 aagggcgcgg cgagaccgac gacatggcgc tcctgctcga tcacatgcag gaacgctatc
     4681 ccggcctgcc ggttgcgctt tcgggcttct cgttcggcac cttcgtccag tcgcagctgc
     4741 agcagcggct gatcgccgcg ggccgcccgg ccgagcggct ggtcctggtc ggctcggccg
     4801 ccggcaagtg ggcgatgccc gacatccccg ccaacaccat cctcatccac ggcgaatacg
     4861 acgagacgat cccgctgtcc gcggtgctca actgggcccg cccgcaggac atcccggtga
     4921 tcgtgattcc cggcgccgat catttttttc accgcaagtt gcaacacata aagaatttcg
     4981 tggtcgaaat gtggcatcgc tagccagccg gcaacatttc gcgccataaa caggaattgc
     5041 acgggaaaac tggctgattg cggtcaactt gtcgcggctg ggacgcatat aatgttccag
     5101 ctagtttcca accgacatcc gctgctgcgg acgcctcatc cagatctccc atgaaaaaac
     5161 tgatcgcggc catcgccgcc accgcctttt cggtctccgc cgcctgggcg caagccctgc
     5221 ccgcccccac catcgccgcc aagtcctggc tgctgctgga tgcgaccagc ggccaggtac
     5281 tggcttccca tgagccgacg atgcgcatcg agccggcttc gctgaccaag atcatgaccg
     5341 cctacctggt cttctcggcg atcaaggaga agcgcctcga tcccgagcag ctgatcaccg
     5401 tctcggtcaa tgcctggaag gtcgatccgg gcagctcgaa gatgttcatc gagccgcgcg
     5461 tgccggtgtc catcaatgac ctgctgtatg ggctgatcgt ccagtccggc aacgacgccg
     5521 cgatcgcgct ggccgaggcc gtggccggca ccgaagaagc cttcgtgcag ctgatgaacc
     5581 gcgaagccga gcgcatgggc ctgaagtcca cgcgcttttc caacccgcac ggcctgacca
     5641 gcaacgacac ctactccacc gcgcaggatc tggccacgct ggcgcatcgc ctgatcaacg
     5701 accatccgga tttcttcagg atctactcga cgcgcgagtt cacgtacaac aagatcaagc
     5761 agcagaaccg caaccgcctg ctgtggctgg acccgacggt cgacggcatg aagaccggct
     5821 acaccgcggc cgccggcttc tgcctgatct ccaccgcgcg ccgcccgaac ggcgaccatg
     5881 agcgccgcct gatctcggtc gtgctcggca ccgcttccga cgcgctgcgc gcccaggaaa
     5941 gccagaaact cttgaactgg ggcttccaga acttcgacac cgtgcgcctg ttcgccaagg
     6001 gcgagccggt cgccacgccg caggtgtgga agggttcgct gaacaaggtg aaggtcggct
     6061 tcacccagga catcttcgtc accgtcccga agggtcaggg cgagcgcatc aagcccgtgc
     6121 tggaacgcac cgacccgctg atcgcgccgg ttgcacagta cagcaacatc ggcacgctga
     6181 agatgatggt cgacggcaag ccgatcgccg agctgccggt ggtgtcgctg gagcaggtga
     6241 accaggccac cgtgttcggc cgcgcatggg actccgtgcg tctctggata aagtaaggtt
     6301 cggctccaat cgcttgcatg ccggcctcgc gccggcatgt tgctttgtgc ggcgtgcaac
     6361 atcacgtgtc agaatgcgcg cttcacggaa acacctcacg taaaaggcca cgcatgcaag
     6421 acccgctcgt ctacctgaac ggtgaactga ccccgctatc gcaggcgcgc attcccgtgc
     6481 ttgaccgcgg cttcatcttc ggcgacggcg tctacgaggt catgccgctg tacaggcgca
     6541 agccgttccg cgcgaagcag cacatggcgc ggctgttccg cagcctgagc gaaatcggta
     6601 ttgccaatcc gcatacggaa gatgattgga tggggctgat cagcagcgtc gccgatgcat
     6661 ttcccgccga cgatcagctg gtctacatcc aggtcacgcg tggcgtggcc aagcgcgcgc
     6721 atgcttttcc gaaggacgtg cagcccacgg tcttcatcat gaccaacccg ctggtgcttc
     6781 ctaccgacgc gcagcgggca gacggtctgg cctgtgtgac attcgaggat cgccgttggt
     6841 tgatgtgcca catcaagtcg atctcgctgc tgggcaacgt gctcgccgcc cagcatgcgg
     6901 ccgagcaggg cgcagccgaa gtgatccagt ttcgcgacgg gttcctcagc gagggcgcgg
     6961 cttcgaatgt gtggatcgtc aaggacggca cgttgatggc gcctccaaaa gacaacctga
     7021 ttctcgaggg catccgctac ggtctgctgg aagagttgtg cgaagcaacc ggcacgcctt
     7081 ttgaagcgcg caggatttcg cgcgaggaag tgtttgcggc ggatgaggtg ctgatgtcgt
     7141 cggcatccaa ggaagtgatt gccgtcacca gcatcgacgg caagccggtc gccgacggca
     7201 agcccgggcc ggtgtacagc cgcctgctcg cggcgtatcg tgaaaagacg ctttgaacta
     7261 agctgattgc tggcgaggct cgcgtttttc ggctcgcgat accgtcaact gaccatcgaa
     7321 caagcccccc ttctcaggga gagggggcag gtagccagaa tacgagccac acctgttcgc
     7381 ctccctgccg ccaaccaccg acacgccatg accgaccaac gtgacagcct gatcgaatac
     7441 ccgagcgact ttcccatcaa gatcatgggc gtgatgcagg acgaatttgc ccagaccatg
     7501 gtggacgtcg tcgtgcgcca tgacccgacc ttccacgccg gcaggatgga gatgcgcgca
     7561 tccagcaagg gcacctatct ctccctcacc gtcaccgtgc tggccaccag ccgcgagcaa
     7621 cttgataact tgtaccgtga gctgagttcg catccgatgg tgaaggtggt gctgtaacga
     7681 ggatagtgcg ctttgcgcgg tgtgaaccgg tgactccaga caagcgcagt ctcgtcacat
     7741 tccctgttcc cgccttggcc gcagcgagtg tgcaaacgca agcggggcaa gcccggccac
     7801 atgcggctat atgaagcacc gacattccgt cgtcccggcg acgtgtcggg cgtgcatcag
     7861 cgccactgga ccccggtttt cgccggagtg acgtccggct atccccgcat cgtcacaaac
     7921 tcctccgcaa tcgtcggatg gatgccgatc gtggcgtcaa actgcgcctt ggtcgcgccg
     7981 cattgcaggg cgaccgcaaa accctggatg atttccccgg catccgcacc caccatgtgc
     8041 acgcccagca cccggtcgct cgcttcgtcg accaccagct tcatcatcac gcgctcgctc
     8101 gcgccgctga tcgtgtgctt gagtgcggtg aattccgact tgaacacgcg caccttcgca
     8161 tgccgttcgc gcgcggtcgc ctcggtcaag ccgacggtgc cgacgttcgg atgactgaac
     8221 accgccgtcg ggatgttttc gtaagacagc ttgcgcgcgc cgtcgcagaa cagcagcgat
     8281 gccaccacca tcccctcggc gagcgcgacc ggcgtgaggg cgacgcgatc gatcacgtcg
     8341 ccgacggcgt ggatcgatgg cacgctggtg gtgaagttcg catcgacctt gatcgcgccg
     8401 cgctcggaca gcctgacgcc gacgttttcc agtcctaggc cttctgtatt tggcacgcgg
     8461 ccggttgcaa acagcactgc ctcggcgtcg agtgtctcac cgttgtcgag caatgcgcgc
     8521 ctgatgccct gcccgtccga tgccagcgat gcgatcaccg tcccgccctt gatcgcaatg
     8581 cccttcttct ccatctcctt gccgagaaag ctgccgatgt cggcgtccat cgcgcgcaac
     8641 agccgcgcgc cgcgatgcac gagcgtgact tggctgccga ggccgttcag gattgcggcc
     8701 agttcgacgg cgatatagcc gccgcctgcg atgacggtcc gcttcggcac gtcgtcgaga
     8761 tggaaaaaat cgtcggatac gatcccgagc gccttgcccg taatttcggg aaccgacggg
     8821 cgcccgccgg tcgcgaccag gatatgccgc gccgtgaccc gcttgccatc gatgttgacg
     8881 gtatgcgggt cttcgatgac cgcattgcct tcatggaccg tcacgccgga tttctcgagc
     8941 agcgaccggt atacgccatt gagccggctg atttcggcgt ccttgttgcg aagcagggtc
     9001 ttccagtcga acgcagcggg cccgacgtcc cagccatagg cgggtgcatg ggccagatcg
     9061 tcgcggtaat gggcggcata ggacatcagc ttcttcggga tgcaaccggc attcacgcag
     9121 gttccgccca gcttgtgccg ctcggccagg gccacgcgcg caccgtattg cgcggcaaag
     9181 cggctcgcgc gcaccccgcc cgagccgccg ccgatcgtga acaggtcaac gtcaaatcgc
     9241 gtattcgtca tgttcgctcc ctcgctttgc aaacgaccga aaccgccatt gagcagcggc
     9301 acggaaaggc tgtattacgc gatacattag ccgtgattca aaaacccggc tctgcaccgc
     9361 ttcagcacac catcccctgc gcctctccca tcacccactc tcggaatgcc tgcacctgcg
     9421 gcttgaccag cgcctcgggc gggcagacga aaaagtagga ctgcgggcac ggaatcgcca
     9481 catcgaacag ccgcaccagc tgccccgact cgatctcgcc aacagccagc acatggcgtg
     9541 ccagcgcgat gccattgcct tccaccgccg cgcgcaccag ttgcgaagaa tcctggaaca
     9601 ccaggccgcc ggtcggctcg agcagatcga gcccggccgc ccggaaccat ggcgtccacg
     9661 gttccagttc acagcgcaac agcggcatat gcttcagctg ctgcggcgtc ttcgggagcc
     9721 tgccgccatt gaaacggggg ctggcgaccg gatagaaggc gtcgtccatc agtttttcca
     9781 cgttcaggcc gggatacttc cccaagccga agcgcagacc cacatcaacc gactcgcgca
     9841 cgaaatcggt cagcgcgtgg ctggactgca gcatgacttc caggtcggga tggcggtcga
     9901 tgaaggcccc cagccgcggg gccagccagc gtgatgcaag cgacggcagc gcggtgatgg
     9961 tcaggcgctg ctggcgcgcg gtgatcttca gcgcttctgc cgccgcggcg acatcgccaa
    10021 gcgcacgccg tacgacagcg gcgaactgct cgccttccgg tgtgaccgtg atgcgcttgc
    10081 catgccgtgc aaacagcgcc acgcccagtt cctcctcgag cgcacgcacc tggtggctga
    10141 tcgctccatg cgtgacatgc acctcttccg cagcgcggga gaaattgccg tgccgcgcgg
    10201 cggcttcgaa ggcgcgcagg gcggccaggt tgggaaggcg gcggaagtcg ctcatggcat
    10261 gtgagatcgg ctaacaagaa agtcgaatat atatcgttta gacaagctag gttcaagtcc
    10321 tagaattccg tcaccccctt gcgtccgtgt ggcttccgtc ggaggccgga cgggggagca
    10381 atggaaggaa ccggaaatca tgagagacct gttcacaagc gaagcaatcc acctggaggc
    10441 cggcgaggtg ctgtccggcg cagccaagcg cgcgcgcatc ctgcgcatca agagcggcag
    10501 cgcctgggtc acagtggagg ggaaccgcta cgattactgg ctcagcgccg gcgactcgct
    10561 gcgcgtggcc accggcaggc tggtggtcgt cgaggcccag acgggcggtt tcgaggccca
    10621 gacacgggtg gaaaccgcgc cggcgccgtt ggcgaccgaa ggcatgctgc gtgcgctgct
    10681 cggcacgctg cggaggtggc ttcgccagga ccgcgtaacc gcaccgacgg tcgaacgccg
    10741 ctgctgcgca taggccaatc atgttccccg ccgatatctg gctggcctat ac
//
LOCUS       DATLGF010000009         8045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_63522,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000009 DATLGF010000000
VERSION     DATLGF010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 8045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8045
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63522"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..349
                     /locus_tag="VM406_00280"
     CDS             77..349
                     /locus_tag="VM406_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74422.1"
                     /translation="MDDDLDGMTRDALVAEVKRLRAGIRAHRDSSGHGLCWHHPQLWG
                     LLPEKTDPVPVVPEWPVFMRGCIRYRQSLDEQAPHAPRSDRPYEGE"
     gene            complement(413..769)
                     /locus_tag="VM406_00285"
     CDS             complement(413..769)
                     /locus_tag="VM406_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HVL74423.1"
                     /translation="MNDLDTLTQMQLRFRDARNWKQFHSLRNLIVSLNLEAAELLELT
                     QWKDDAALASALQDAQFREMLRDECADVLLYLVLVAEAGGVDLMEAARAKIAKNALKY
                     PVEKAYGSARKGEAGT"
     gene            complement(813..1706)
                     /locus_tag="VM406_00290"
     CDS             complement(813..1706)
                     /locus_tag="VM406_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL74424.1"
                     /translation="MLPDLEFFVLLARHRSLSAAARALDITPPAATRRLAQLEERLGV
                     RLVNRTTRSVSLTGEGQTYLEYATRILAELREMEEAVSRSRAAPQGLLRVNATLGFGR
                     TTIAPLVSAFVKGHPGVEVQLDVTDRPVDLVESGYDLAIRFGALPDRRLSARRLMSNR
                     RFLCASPRYLKQHGTPETIADLARHRCIIHRQDNDAHGIWRFLHGEHSEAIKVHGTVS
                     SNDGDIVLGWALDGHGILIRSEWDLAKYLESGRLHVVLPKLRLPPADLTVYYPAGRNV
                     SARMRAFIDFLVEHVQARARS"
     gene            1805..2971
                     /locus_tag="VM406_00295"
     CDS             1805..2971
                     /locus_tag="VM406_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011660310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HVL74425.1"
                     /translation="MRIVDIREKTFPISSPIRNAYIDFSKMTLSLVAVITDVVRNGRP
                     VVGYGFNSNGRYGQGSLMRERFIPRILEAAPESLLDDSGENLDPHKIWNCMFTNEKPG
                     GHGERSVAIGTIDMAVWDAVAKIAGKPLYQLLAERYGNGTPNREVFVYAAGGYYYPGK
                     NDDALKDEMRSYLDRGYNVVKMKIGGASLDVDLRRIDAALSVLQDGQKLAVDANGRFD
                     LDTAIAYAKALSRYDLFWYEEAGDPLDYELQATLRNYYDKPMATGENLFSMQDARNLI
                     RYGGMRADRDWLQFDCALSYGLVEYLRTLDMLHQHGWSAARCIPHGGHQMSLNIAAGL
                     GLGGNESYPDLFQPFGGFPDGVKVENGRITMPELDGIGFEGKANLYAVMRELAA"
     gene            complement(2972..3853)
                     /locus_tag="VM406_00300"
     CDS             complement(2972..3853)
                     /locus_tag="VM406_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVL74426.1"
                     /translation="MPLLQPLSRLLRPRRYRFAAAGAALASVLAAAGCGQLAQKEREL
                     TFRIEPGNASWYSGIPEGLVEKYIPVGNGEERLHTWWWPAEREHAPTLLYLHGARWNL
                     TGHLPRIALLRDLGFSVLAIDYRGFGRSGGELPSERTVYEDARIAWAYLVGLQPDPAR
                     RFVYGHSLGGAVAIELATALDAQDAGGVIVESTFTSLADVARSLSYPWLPLQLVMSQR
                     FDSVSKISRVDLPLLVVHGSEDRVVSPHFGAKLYEAAGGSKRLLMVEGAGHNNGMRLA
                     QREFREALGELFPIPAL"
     gene            complement(3949..4536)
                     /locus_tag="VM406_00305"
     CDS             complement(3949..4536)
                     /locus_tag="VM406_00305"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HVL74427.1"
                     /translation="MSKRILLIQGHPDASARHFGHELEDAYAEGALALGHEVRRIQIA
                     KLDFPMLRSQYDWEKGELPASLAESQEAIKWCEHLVLFFPLWLGDMPALMKAYLEQVA
                     RPGFAFGEAGTASFGNKGLLGRSARVVVTMGMPAMIYRWYFRAHSVKSLERNILGFVG
                     FSPVNETLIGNVGSIDAKSAERWIEKLATLGGLAE"
     gene            complement(4607..5764)
                     /locus_tag="VM406_00310"
     CDS             complement(4607..5764)
                     /locus_tag="VM406_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74428.1"
                     /translation="MPLSSSQRSLLPLVGLQGAVHSLGGFIGFFVVGEHSIHALLRYT
                     AGMMTAAMLATFIAYAFGSRLGLSARGLLRLGFVLPGVLLLAAGTSVAALALAFGTFL
                     GMTWSARHALEMSLMQNRERDGYAAHAVAMMLVPALLATLAATVILALGGQSAQHVYW
                     FYGAMCVAGGLLLGRGIPDTEPVALKAPLAVLRQPQFIACLPLFFLESGLFGISQAMA
                     AAGAVQALGSASQFGWVATVAGLVGGCALYLTRRQRDIHNRAGWLGAACLVIGLSYVL
                     LGASAWLPALFVVHAVTRAAGMPFLSASQQVMNQCTLDVRGELSDRIFARELVLWLLR
                     MISLGLFWLLAMALTPIQLLVAGASVLACATALEYLLGKSLFGRNLAHQPA"
     gene            6069..6881
                     /locus_tag="VM406_00315"
     CDS             6069..6881
                     /locus_tag="VM406_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVL74429.1"
                     /translation="MLTTPLSEVRASSLPFAATGGAAWQRQAKLWSSLKEVCDLLRIP
                     AKIQNDDFQFQHMQFKAGQRIHTIGQMFDTLYIVNSGFLKTVMIDEIGNEQVLSFPMK
                     GDLFGIDGIHSKQYSTETVALTNCDIILVPFKMFASLGRTQPDLEAAIYAVMSRELVR
                     EQAMISTLGSLSAEARVARFLAFLSTRFTEMGYSGTQFNLRMTRQEIGNYLGLTLETV
                     SRTFSAFNAIGLISVDQRAIQIHDLQALKTLRRLPPSRSRAKAKQPSVPLAA"
     gene            complement(6963..>8045)
                     /locus_tag="VM406_00320"
     CDS             complement(6963..>8045)
                     /locus_tag="VM406_00320"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HVL74430.1"
                     /translation="ALAARLGGDEFTVLLDDPPGRREAEALGERLMSVLAMPFQIEGQ
                     EMFVTASIGAARFPEDGDDAASLLKNADVAMYRVKQRGRNNFQFFTRDMNGAGLEQLM
                     LENGLRRALERGEFELHYQPQMCTRTGRIGGVEALIRWRRPEVGLVPPGAFIGLAEQN
                     GLIMTIGAWVLREACRQAKEWLDQGFEFGRVAVNLSARQFESGDLLQTIRDALYVTGL
                     PPTRLELEITESIIMRNPQEAIGVLERVRDLGIAIAVDDFGTGYSSLASLKQYPLDRL
                     KIDRSFISGIPEDADDIAITEAIVAVGHKMGLELVAEGVETAAQRDFLRKAGCDFAQG
                     YLLGRPVLPEALQERLAQVAGPASAA"
BASE COUNT         1418 a         2675 c         2646 g         1306 t
ORIGIN      
        1 gtactacacg tactgtctgc gtcggcgcgc cttgccatcg gcttctgtta gggcctctaa
       61 gttgcaggag gaaggcatgg acgacgatct cgacggcatg acgcgcgacg cgctggtcgc
      121 ggaagtgaag cgcctgcgcg cgggcatccg cgcgcatcgc gacagcagcg ggcatgggct
      181 gtgctggcat catccccagc tctggggctt gctgccggaa aagaccgatc cggttccggt
      241 ggtgccggaa tggccggtat tcatgcgcgg ctgcatccgc tatcggcagt cgctcgatga
      301 gcaggcgccg catgcgccgc gcagtgacag gccgtatgag ggggagtgag gggcgcggct
      361 ctttcgccta cgtttctcaa gttcgggggc gtcgcgacgg gcgcatggcg gactaggttc
      421 ccgcctcccc cttgcgcgcc gacccatacg ccttctccac cggatacttc agcgcattct
      481 tcgcgatctt cgcgcgcgcc gcctccatca ggtccacgcc gccggcctcg gcgaccagca
      541 ccagatagag cagcacgtcc gcgcactcat cgcgcagcat ttcgcggaac tgcgcatcct
      601 gcagcgcgga agcgagcgcc gcatcgtcct tccattgcgt cagttccagc agttccgccg
      661 cttccaggtt cagcgaaacg atcaggttgc gcagcgaatg gaattgtttc cagttgcgcg
      721 cgtcgcgaaa gcgcaactgc atctgcgtga gggtgtcgag gtcgttcatg atcgtgttgg
      781 ttgaggagat gcgatcgatt gtgccagcgc gttcagctgc gtgctcgcgc ctggacgtgc
      841 tcgacgagga agtcgatgaa cgcgcgcatc cgtgcggaga cattgcgccc ggccggatag
      901 tagacggtca ggtcagccgg cggcagccgc agcttgggca gcaccacgtg cagccgcccg
      961 ctttccaggt atttcgccaa atcccattcc gaacggatca ggatgccgtg gccgtccagc
     1021 gcccagccga gcacgatgtc gccgtcgttg ctggacacgg tgccatgcac cttgatcgcc
     1081 tcgctgtgtt cgccgtgcag gaagcgccag ataccgtggg catcgttgtc ctggcgatgg
     1141 atgatgcagc gatgccgggc gaggtcggca atcgtctccg gcgtgccgtg ctgcttcagg
     1201 tagcgcggcg atgcacacag gaagcgccgg ttcgacatca gccggcgcgc ggacagccgc
     1261 cggtcgggca gggcgccgaa gcggatcgca agatcgtagc cgctttccac caggtccacc
     1321 ggccggtcgg tcacgtcgag ctgcacctcc acgcccgggt ggcccttgac gaaggccgat
     1381 accagcggcg cgatcgtcgt gcggccgaaa cccagcgtcg cgttgacgcg cagcagtcct
     1441 tgcggcgcgg cacggctgcg cgagaccgcc tcctccatct cgcgcagctc ggccaggatg
     1501 cgggtggcgt actcgagata ggtctggccc tcgccggtca ggctcacgct gcgcgtggtg
     1561 cgattgacca ggcgcacgcc cagacgctct tccagctggg caagccggcg cgtggccgcc
     1621 ggcggcgtga tgtcgagcgc gcgcgccgcc gccgacaggc tgcggtggcg cgcaagcaac
     1681 acgaagaact cgagatcggg caacacgatt attcaggaaa agtgaaaaga aaagtgagtt
     1741 gccgatcaat tatagcctga ccgcccggcg ctattctgca gctgttcaac caagggaaac
     1801 cgccatgaga atcgtcgaca tccgcgaaaa aacctttccg atcagttcgc ccatccgcaa
     1861 tgcctacatc gacttcagca agatgacgct gagcctggtg gccgtcatca ccgatgtggt
     1921 gcgcaatggc cgtccggtgg tcggctacgg cttcaactcg aacgggcgct acggccaggg
     1981 cagcctgatg cgcgagcgct tcattccgcg catcctcgaa gctgcgccgg aatcgctgct
     2041 ggacgacagc ggcgagaacc tcgatccgca caagatctgg aactgcatgt tcaccaacga
     2101 aaagccgggc ggccatggcg agcgctcggt cgccatcggc acgatcgaca tggcagtgtg
     2161 ggacgcggtc gcgaagatcg ccggcaagcc gctgtaccag ctgctggccg agcgctatgg
     2221 caacggcacg ccgaaccgcg aggtgttcgt ctatgcggcc ggcggctact actatccggg
     2281 caagaacgac gacgcgctga aggacgagat gcgcagctac ctggaccgcg gctacaacgt
     2341 ggtgaagatg aagatcggcg gcgcgtcgct cgacgtcgac ctgcgccgca tcgacgcggc
     2401 actgtcggtg ctgcaggacg gccagaagct ggcggtcgat gcgaacggcc gcttcgacct
     2461 cgacaccgcg atcgcctacg cgaaggcgct gtcgcgctac gacctgttct ggtacgagga
     2521 agcgggcgac ccgctcgact acgagctgca ggccacactg cgcaactact acgacaagcc
     2581 aatggccacc ggcgagaacc tgttctcgat gcaggatgcg cgcaacctga tccgctacgg
     2641 cggcatgcgc gccgaccgcg actggctgca gttcgactgc gcgctgtctt acggcctggt
     2701 cgaatacctg cgcacgctcg acatgctgca ccagcacggc tggtcggccg cgcgctgcat
     2761 tccgcacggc ggccaccaga tgtcgctgaa tatcgccgcg ggtcttggtc tgggcggcaa
     2821 cgaatcctat ccggacctgt tccagccctt cggtggcttc cccgacggcg tgaaggtgga
     2881 gaacgggcgc atcacgatgc ccgagcttga tggcatcggt ttcgaaggca aggcgaacct
     2941 gtacgcggtg atgcgcgaac ttgcggcctg atcagagcgc ggggatcggg aagagctcgc
     3001 ccagcgcttc ccggaattcc cgctgcgcga gccgcatgcc gttgttgtgg ccggcgcctt
     3061 ccaccatcaa cagccgcttg gatccgccgg cggcctcgta cagcttcgcg ccgaagtgcg
     3121 gcgagacgac ccgatcctcg ctgccgtgga cgaccaggag cggcaggtcg acgcgggaaa
     3181 tcttgctcac cgaatcgaaa cgctgcgaca tcaccagctg cagcggcagc cagggatagc
     3241 tgagcgagcg cgccacatcg gccagggagg tgaaggtgga ttcgacgatg acgccgcccg
     3301 cgtcctgtgc atcgagcgcg gtggccagtt cgatcgccac cgccccgccc agcgagtgtc
     3361 catagacgaa gcgccgcgcg ggatcgggct gcagcccgac cagatacgcc caggcgatgc
     3421 gtgcgtcttc atagacggtg cgttcggacg gcaattcgcc gccgctgcgg ccgaagccgc
     3481 ggtagtcgat tgccagtacc gagaacccga ggtcgcgcag cagggcaatg cgcggcaggt
     3541 ggccggtcag gttccagcgc gcgccgtgca ggtagagcag ggtgggcgcg tgctcgcgct
     3601 cggccggcca ccaccaggtg tggagccgtt cttcgccatt cccgaccggg atgtattttt
     3661 cgaccagccc ctcggggatg ccgctgtacc agctggcgtt acccggttcg atgcggaagg
     3721 tgagttcgcg ctccttctgc gcgagctggc cgcagccggc ggcggccagc acggatgcga
     3781 gggcggcgcc ggcggcggcg aagcggtagc gacgagggcg aaggaggcgg gacagaggtt
     3841 gcagaagcgg catgaccgag cggctcaggt tcttggtatt gcgaaagatg cacgcggtta
     3901 cgacctcttg ccgcaggaaa cgttcctgtc cgctatcgag ccatggttct actcggcgag
     3961 tcccccgagc gtggccagct tctcgatcca tctttcggcg ctctttgcat cgatgctgcc
     4021 gacattgccg atcagtgtct cgttgaccgg cgagaagccg acgaagccga ggatgttgcg
     4081 ctcgagcgac ttgacgctgt gcgcgcggaa gtaccagcgg tagatcatcg ccggcatgcc
     4141 catcgtgacc accacgcggg ccgagcgtcc gagcagtccc ttgttgccga agctcgccgt
     4201 gccggcctcg ccgaatgcga agccgggccg ggcgacctgc tcgagatagg ccttcatcaa
     4261 ggcaggcatg tcgcccagcc atagcgggaa gaacagcacg aggtgctcgc accatttgat
     4321 cgcctcctgc gattccgcca ggctggcggg cagctcgccc ttttcccaat cgtactggct
     4381 gcgcagcatc gggaaatcca gcttggcgat ctggatgcgg cgcacctcgt gtcccaatgc
     4441 gagcgcgcct tcggcgtagg cgtcttccag ttcatggccg aagtggcggg cgctggcgtc
     4501 ggggtggccc tggatcagga gtatgcgttt gctcatggat tgacctgctg tcgatagacg
     4561 ggcaggttcg catccgggcg cagccgaaac cttgatctgc cgcaagtcac gccggttggt
     4621 gcgccaggtt gcgcccgaac agcgacttgc ccagcaggta ttcgagggcg gtcgcgcagg
     4681 ccagcacgct cgcccccgcc accagcagct ggatcggcgt gagcgccatc gccagcagcc
     4741 agaacaggcc gagcgagatc atgcgcagca gccacagcac cagttcgcgc gcgaagatgc
     4801 ggtccgacag ttcgccgcgc acatccagcg tgcactggtt catcacctgc tggctggccg
     4861 acaggaaggg catgcccgcc gcgcgcgtga ccgcatgcac gacgaacagc gccggcagcc
     4921 aggcgctggc gcccagcagt acgtaggaca ggccgataac caggcaggcc gcgcccagcc
     4981 agccggcgcg gttgtggatg tcgcgctggc ggcgtgtcag gtaaagcgcg cagccgccga
     5041 ccaggccggc cacggtcgcg acccagccga actggctcgc cgagcccagc gcctgcacgg
     5101 caccggccgc ggccatcgcc tggctgatgc cgaacaatcc ggattcgagg aagaacaggg
     5161 gcaggcaggc gatgaattgc ggctggcgca gcacggcaag cggcgccttc agggcgaccg
     5221 gctcggtatc cggaatcccg cgtccgagca gcaggccgcc ggccacgcac atcgcgccgt
     5281 agaaccagta cacgtgctgc gcgctctggc cgcccagcgc gagtatcacg gtcgcggcca
     5341 gcgtcgccag cagcgccggg accagcatca tcgccaccgc atgggccgca tacccgtcac
     5401 gctcgcggtt ctgcatcagc gacatctcca gcgcgtgacg cgcgctccag gtcatgccca
     5461 gaaaggttcc gaatgcgagc gccagtgcgg ccaccgacgt gccggcggcg agcagcagca
     5521 ccccaggcag cacgaagcca agacggagga gcccccgggc ggacaagcca agccgcgagc
     5581 cgaaggcata ggcgatgaag gtagccagca tcgcggcggt catcatgccg gcggtgtagc
     5641 gcagcagtgc atgaatgctg tgctcgccga ccacgaagaa accgatgaag ccgccaagcg
     5701 aatggactgc gccttgcagg ccgacaagcg gcagcagcga gcgttgcgag gatgacaggg
     5761 gcatgtggaa gagctgcgtc gggggaacgc gcttcggaca tgccgggcgg caatttgttc
     5821 cgaggaaacg gaatggcgcg cgcggcctgg gcgcgggcat gcctcaaggc agcgccgaat
     5881 gagcgtcgcg ccgcgaggag tcctgtagga atcgccttac tgcgctcctt gttgtccgac
     5941 agtgcatttc atcccacgct gatttccatg caaccttgcg gggaacataa tcctttcacc
     6001 gattgccgat tcgcaaatca agtggatcaa acgaaaaacc aacccgccac ataaataagg
     6061 aaaacgagat gctgactact cccctgtctg aagtgcgtgc ctcctccctg ccttttgccg
     6121 cgaccggcgg cgccgcgtgg caacgccagg ccaagctgtg gtccagcctg aaggaagtgt
     6181 gcgacctgct gcgcattccc gcgaagatcc agaacgacga tttccagttc cagcacatgc
     6241 agttcaaggc aggccagcgc atccatacga tcggccagat gttcgatacg ctgtacatcg
     6301 tcaactccgg cttcctgaag accgtgatga tcgacgagat cggcaacgag caggtgctct
     6361 cgttcccgat gaagggcgac ctgttcggca tcgacggcat ccactccaag cagtattcga
     6421 ccgagaccgt cgcgctgacc aactgcgaca tcatcctggt gccgttcaag atgttcgcct
     6481 cgctgggccg cacccagccc gacctggaag ccgcgatcta tgcggtgatg agccgcgagc
     6541 tggtgcgcga gcaggcgatg atcagcacgc tgggttcgct gtcggccgaa gcccgcgtgg
     6601 cgcgcttcct ggccttcctg tcgacccgat tcacggaaat gggctattcc ggcacccagt
     6661 tcaacctgcg catgacgcgc caggagatcg gcaactacct cggcctgacg ctggagacgg
     6721 tttcccggac gttctcggca ttcaacgcga tcggcctgat ctcggtcgac cagcgcgcga
     6781 ttcagatcca cgacctgcag gcgctgaaga ccctgcgccg cctgccgccg tcgcgctcgc
     6841 gcgccaaggc caagcagccg tcggtgccgc tggcagcata agcgcagctc ccgtttcact
     6901 ccctcccgag cccgccgggc ggtcctgtgg ccgtccggtt ttttttcgtc cgccgctggg
     6961 gctcaggccg ccgaagccgg gccggccacc tgcgccagcc gctcctgcag ggcctccggc
     7021 agcaccggcc ggcccagcag gtagccctgc gcgaagtcgc atccggcctt gcgcaggaag
     7081 tcgcgctgcg cggccgtttc cacgccctcg gcgaccagct ccagtcccat cttgtggccg
     7141 accgcgacga tggcttcggt gatcgcgatg tcgtcggcgt cctcggggat gccggaaatg
     7201 aagctgcggt caatcttcag gcggtccagc ggatactgct tcagacttgc cagcgacgaa
     7261 tagccggtgc cgaaatcgtc gaccgcgatc gcgatgccga gatcgcgcac gcgctcgagc
     7321 acgccgatgg cttcctgcgg attgcgcatg atgatgctct cggtgatctc cagttccagc
     7381 cgcgtcggcg gcagtccggt cacgtacagc gcatcgcgga tcgtttgcag caggtcgccc
     7441 gattcgaact ggcgcgccga caggttgacc gccacgcgcc cgaactcgaa accctggtcc
     7501 agccattcct tggcctggcg gcaggcctcc cgcagcaccc aggcgccgat cgtcatgatc
     7561 aagccgttct gctcggccag cccgatgaag gcgccgggcg gcaccagccc gacctccgga
     7621 cggcgccagc ggatcagcgc ttcgacgccg ccgatgcggc cggtgcgcgt gcacatctgc
     7681 ggctggtaat gcagctcgaa ctcgccgcgc tcgagcgcgc ggcgcaggcc gttctccagc
     7741 atcagctgct ccagcccggc gccgttcatg tcgcgcgtga agaactggaa gttgttgcgc
     7801 ccgcgctgct tgacgcgata catcgccacg tcggcattct tcagcaggct ggccgcgtca
     7861 tcgccgtcct cgggaaagcg cgccgcgccg atgctggccg tcacgaacat ctcctggcct
     7921 tcgatctgga acggcatggc cagtacgctc atcaggcgct cgccgagcgc ctcggcctcg
     7981 cggcggcccg gcgggtcgtc gagcaggacc gtgaattcgt cgccgcccag gcgcgcggcc
     8041 agcgc
//
LOCUS       DATLGF010000010         5473 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_158546, whole genome shotgun sequence.
ACCESSION   DATLGF010000010 DATLGF010000000
VERSION     DATLGF010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158546"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..1592
                     /locus_tag="VM406_00325"
     CDS             15..1592
                     /locus_tag="VM406_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HVL74431.1"
                     /translation="MKLVEVPQQAPAPEIPEIEARAPAPDKTAPAKRTAKSKPKTAES
                     QIKSTTSKTADKLGVTKMFVLDTNVLMHDPTALFRFEEHDIYLPMITLEELDDHKKGM
                     SEVARNARQVSRTLDALVSGVEEDAIEAGIPLSKLGNADAKGRLYFQTQLRGVKAPEG
                     LPVGKADNHILGVVQALEALHPGRPIVLVSKDINMRIKARAMGLPAEEYFNDHVLEDT
                     DLLYSGITQLPDDFWHKHAKGMESWQENKHGTGYTFYRVTGPAVPSLLINQFVFLEPS
                     NGEAPFYGQVRQINGKTAVLQTLRDYTHGKNSVWGITARNREQNFALNLLMNPECDFV
                     TLLGQAGTGKTLLALAAGLAQVLETKLYNEIIVTRVTVPVGEDIGFLPGTEEEKMGPW
                     MGAFDDNLEVLNKSDHDAGEWGRAATQDLIRSRIKIKSLNFMRGRTFVNKFLIIDEAQ
                     NLTPKQMKTLVTRAGPGTKIICLGNIAQIDTPYLTEGSSGLTYVVDRFKGWAHSGHVT
                     LARGERSRLADHASDVL"
     gene            complement(1714..2721)
                     /locus_tag="VM406_00330"
     CDS             complement(1714..2721)
                     /locus_tag="VM406_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003266172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HVL74432.1"
                     /translation="MYILDPGLYFVVLLIGGALAFGFMNALHDAADAIATVVSTRVLK
                     PYQAVVLAATCNGLAILFFPLFVAALLATGTVQELAADQYVVSGALAGAIAWLLLTWM
                     FKIPSSATHALVGALLGATVSKGGSEALITSGVAKILAFVLLSPLLGFVLGTGAVIVV
                     SWLFVRTTPRRVDKGFRRMQLLSAGLYNLGHGANNAQKALGMIWMLLLAAGALQANDM
                     PPYWAVVSSYAVVAAGTLLGGWRVVKTMGQKITRLKPVSGFCAEIGGALTMILANNIG
                     APISPRQALTGALVGVGASQRMTTVRWAVVGNMVWAWVITIPASAFAGAIAWWLAHRV
                     L"
     gene            complement(2735..3361)
                     /locus_tag="VM406_00335"
     CDS             complement(2735..3361)
                     /locus_tag="VM406_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HVL74433.1"
                     /translation="MFGRLMPREDKFFDLFVRHGDLCIKGAKEMVALMTDFNDLEHRA
                     HAIEVIEKQADKLTHETLDLLRTTFITPLDRDDIHKLITRMDDILDLLEDAGQTISLY
                     DIQTVTPEAKRLAELCLAGAEKVRQAVGLLQNMDNSVQILALCEEIDRVESDADHVLR
                     AAMSKLFRDEPDVRTLIKLKAIYEILETVTDRCEDVANIIEGIIVDNA"
     gene            complement(3389..4774)
                     /locus_tag="VM406_00340"
     CDS             complement(3389..4774)
                     /locus_tag="VM406_00340"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HVL74434.1"
                     /translation="MNDRADPQLDALRVPPHSIEAEQSVLGGLLLDNSAWDRIADFVN
                     EDDFYRYDHRIIFQHIAKLISNTKPADVITVYEALGSSGKADEVGGLAYLNALAQNTP
                     SAANIRRYAEIVRERGVLRKLVTVSDEIASTALNPQGKEVKQILDEAESKIFAIAEDG
                     ARGAQGWLAIQPLLTQVVERIDELYNRDNQNDITGVPTGFIDLDRMTSGLQPGDLVIV
                     AGRPSMGKTAFSLNIGENVAIDSGLPVAVFSMEMGGAQLAMRMLGSVGRLDQSRLRIG
                     KLNDEDWPRLTHAIQKMNEAQLYIDETPGLSPVELRARARRLARQCGKLGLIIVDYLQ
                     LMSGNGSGENRATEISEISRSLKGLAKELGCPLIALSQLNRSLEQRPNKRPVMSDLRE
                     SGAIEQDADVILFIYRDQVYNPDSPDKGTAEIIIGKQRNGPIGTVRLTFLGEYTKFDN
                     YTGGQAIFGGE"
     gene            complement(4897..5349)
                     /gene="rplI"
                     /locus_tag="VM406_00345"
     CDS             complement(4897..5349)
                     /gene="rplI"
                     /locus_tag="VM406_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HVL74435.1"
                     /translation="MQVILLEKVVNLGNLGDVVKVKDGYARNFLIPQRKARRATETAV
                     AEFEAKRAELEKAAAEKLAAAQAQGEKLGGTSVQIGQKAGVDGRLFGSVTNADIAEAV
                     TKQGFAVEKAQIRMPHGPIKMTGEHPVQVVLHTDVVVDITVQVVGEQA"
     gene            complement(5374..>5473)
                     /gene="rpsR"
                     /locus_tag="VM406_00350"
     CDS             complement(5374..>5473)
                     /gene="rpsR"
                     /locus_tag="VM406_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HVL74436.1"
                     /translation="TGTKAHYQRQVDTAIKRARYLALLPYTDLHNA"
BASE COUNT         1071 a         1747 c         1674 g          981 t
ORIGIN      
        1 cggtaaagcc gctgatgaag ctggtagaag tcccgcagca agcacccgcc cccgaaatcc
       61 ccgaaatcga agcgcgcgcc ccggcacccg acaagacggc gccagccaag cgcaccgcga
      121 agtcgaaacc caagaccgcc gaaagccaga tcaagtcgac caccagcaag accgccgaca
      181 agctcggcgt gaccaagatg ttcgttctcg acaccaatgt gctgatgcac gacccgaccg
      241 cactgttccg cttcgaagag cacgacatct acctgccgat gatcacgctc gaggaactgg
      301 atgatcacaa gaagggcatg tcggaggtcg cgcgcaacgc gcgccaggtg tcgcgcacgc
      361 tcgatgcgct ggtgagcggc gtggaagagg atgcgatcga ggccggcatc ccgctttcca
      421 agctgggcaa cgccgacgcg aaggggcgcc tgtacttcca gacccagctg cgcggcgtga
      481 aggcgccgga agggctgccg gtcggcaagg cggacaacca catactcgga gtcgtgcagg
      541 cgctggaggc gctgcatccg ggccgtccca tcgtactggt gtccaaggac atcaacatgc
      601 gcatcaaggc gcgcgcgatg ggccttccgg cggaagagta cttcaacgac cacgtcctcg
      661 aagacaccga cctgctctac tccggcatca cgcagctgcc ggacgacttc tggcacaagc
      721 acgcgaaggg catggaatcc tggcaggaga acaagcacgg caccggctac accttctacc
      781 gcgtgaccgg tcccgccgtg ccttcgctgc tgatcaacca gttcgtgttc ctggaaccga
      841 gcaatggcga agcaccgttc tacggccagg tccggcagat caacggcaag accgcggtgc
      901 tgcagacgct gcgcgactac acgcacggca agaacagcgt ctggggcatc acggcgcgca
      961 accgcgagca gaatttcgcg ttgaacctgt tgatgaatcc ggaatgcgat tttgtcacgc
     1021 tgctcggcca ggccggaacc ggcaagaccc tgctcgcgct ggccgccggc cttgcccagg
     1081 tgctcgagac caagctctac aacgagatca tcgtcacccg ggtcacggtg ccggtcggcg
     1141 aggacatcgg cttcttgcca ggcaccgagg aagaaaaaat ggggccctgg atgggcgcat
     1201 tcgatgacaa cctggaggtg ctgaacaagt ccgatcacga tgccggcgag tggggccgtg
     1261 cggcaaccca ggacctgatc cgctcgcgca tcaagatcaa gtcgctcaac ttcatgcgcg
     1321 gccgcacctt cgtcaacaag ttcctgatca tcgacgaagc gcagaacctg acgccaaagc
     1381 agatgaagac gctggtcacg cgcgccggcc ccggcacgaa aatcatctgc ctggggaata
     1441 ttgcgcagat cgacacgccc tacctgaccg aaggatcgag cgggctgacc tacgtggtcg
     1501 accgcttcaa gggatgggcg cacagcggac atgtgacgct ggcgcgcggc gaacgctcgc
     1561 ggctggccga tcacgcaagc gacgtgctct gagcaggagc gccggcgaaa cgaaaaaggg
     1621 gcctgcatct gcgggcccct tttcattggg ttcttgcttt ccggtgccgg tccgtcgatg
     1681 cgacccgcgg ccgcaacgca gacgaggaca ggatcacaag acccggtgtg caagccacca
     1741 ggcaatcgct cccgcaaatg cggaggccgg aatggtaatc acccaggccc agaccatgtt
     1801 cccgaccacg gcccagcgca ccgttgtcat gcgctgtgac gcacccacgc ccacgagcgc
     1861 cccggtcagc gcctgccggg gcgagatcgg cgcgccgatg ttgttggcca atatcatcgt
     1921 caacgcgccg ccaatctccg cgcagaagcc gctgacaggc ttgagccggg tgatcttctg
     1981 acccatcgtc ttcaccacgc gccatccgcc aagcagcgtg cccgcagcga ccaccgcata
     2041 gctgctgacc accgcccagt atggcggcat atcgtttgcc tggagcgcac ccgcggcgag
     2101 cagcagcatc cagatcattc cgagcgcctt ttgcgcattg ttggcgccat ggccgaggtt
     2161 gtacaggccg gccgacaaga gctgcatgcg acgaaacccc ttgtcgacgc ggcgtggcgt
     2221 ggtacgcacg aacagccagg acaccacgat cactgccccc gtgccgagca cgaatccgag
     2281 caacggcgac agcagcacga atgcgaggat cttcgcgact ccgctggtga tcaacgcctc
     2341 gctgcctccc ttggaaacgg tcgccccgag cagcgcacca accagcgcat gggtggcgga
     2401 agacgggatc ttgaacatcc atgtcagcag cagccacgcg atcgcacctg ccagcgcgcc
     2461 cgaaaccacg tactggtcgg cggccagttc ctgtactgtg ccggtcgcca acagcgcagc
     2521 aacgaacaat ggaaaaaaca ggatggcaag cccgttgcac gtggcggcca gcaccaccgc
     2581 ctggtagggc ttgagcacgc gcgtggagac gacagtggca atcgcgtcgg cggcgtcgtg
     2641 cagcgcgttc atgaagccga aggccagcgc gccgccgatc agcaggacga cgaaatacag
     2701 gcccggatcc agtatgtaca tgagcgatca gcgcctaggc attgtcgacg atgatgccct
     2761 cgatgatgtt cgccacatcc tcgcagcgat ccgttaccgt ctccaggatc tcgtagatcg
     2821 ccttcagctt gatcagcgtg cgcacgtccg gctcgtcgcg aaacagcttc gacatcgccg
     2881 cgcgcagcac atggtcggcg tcggattcga cccggtcgat ctcctcgcac agcgccagga
     2941 tctgcaccga gttatccatg ttctgcagca gcccgaccgc ctggcgcact ttctccgccc
     3001 ctgcgaggca caactcggcc aggcgcttgg cctccggcgt caccgtctgg atgtcgtaca
     3061 gcgaaatggt ctggccggcg tcctcgagca ggtcgaggat gtcgtccatg cgggtgatca
     3121 gcttgtggat gtcgtcccgg tccagcggcg tgatgaaggt cgtgcgcagc aggtccagcg
     3181 tttcatgcgt gagcttgtcc gcctgcttct cgatgacttc gatcgcgtgg gcgcgatgtt
     3241 cgaggtcatt gaagtccgtc atcagcgcga ccatttcctt cgcgcccttg atgcagaggt
     3301 cgccatgacg aacgaacagg tcgaagaatt tgtcctcgcg gggcatcaat cgtccaaaca
     3361 tcggatctct ttcaggtcgt gcgacggctt attcgccgcc gaagatggct tggccgccgg
     3421 tgtagttgtc gaacttcgtg tattcgccaa ggaaggtcag gcgcacggtg ccgatcgggc
     3481 cgttacgctg cttgccgatg atgatttcgg ccgttccctt gtcgggagaa tcggggttgt
     3541 agacttggtc gcggtatatg aacaggatca cgtcggcatc ctgctcgatg gcgccggatt
     3601 cgcgcagatc cgacatcacc ggtcgtttgt tcggccgctg ctcaagcgag cggttcagct
     3661 gcgacagcgc gatcaagggg cagcccagct cttttgcaag gcccttcagg cttcgggaga
     3721 tttccgagat ttcggtagcg cggttttcac ccgaaccgtt gcctgacatc agctgcaggt
     3781 aatcgacgat gatcaggccg agcttgccgc actgccgcgc cagacgccgc gcgcgggcgc
     3841 gcaattcaac cggcgagagg cctggcgtct cgtcgatata cagctgcgcc tcgttcatct
     3901 tctggatcgc atgcgtcagg cgcggccagt cctcgtcgtt cagtttgccg atacgcagac
     3961 ggctctggtc cagccgaccg accgaaccca gcatacgcat ggccagctgg gcgccgccca
     4021 tttccatcga aaacaccgcc accggcaggc cgctgtcgat ggcgacgttc tccccgatgt
     4081 tcagactgaa tgcggtcttg cccatcgaag gacgtccggc gacgatcacc aggtcgcccg
     4141 gctgcaggcc ggacgtcatc cggtcaagat cgatgaaccc cgtcggcacc ccggtaatgt
     4201 cgttctggtt gtcgcggttg tacagctcgt cgatgcgctc gacgacctgg gtcaggagcg
     4261 gctggatggc cagccaaccc tgtgcgccgc gcgcgccgtc ttcggcgatc gcaaatatct
     4321 tcgattcggc ttcgtcgagg atctgcttga cttccttgcc ctggggatta agcgcggtcg
     4381 aggcaatctc gtcggatacg gtgaccagtt tgcgcagcac accgcgctcg cgcacgatct
     4441 ctgcatagcg acggatattg gcagccgacg gcgtgttttg cgccaacgcg ttcaggtaag
     4501 ccaagccgcc gacttcgtca gccttgccgc ttgagcccag cgcctcataa accgtgatga
     4561 catcggccgg cttggtgtta ctgatcagct tggcgatgtg ctggaagatg atgcggtggt
     4621 cgtagcgata gaaatcgtcc tcgttaacga aatccgcgat acggtcccag gccgaattgt
     4681 cgagcagaag cccgccgaga acggactgtt ccgcttcgat ggaatgaggg gggacgcgca
     4741 aggcgtccag ctgcggatcg gcacggtcgt tcatggcgcg caattatact cgctgatact
     4801 cggcaggccg tgctggaaag gcggtccgga ggacgaaaaa cggacggaaa aaaaggccgg
     4861 acaagccggc ctttttcaaa tgttcaacag caacgcttag gcttgctcgc ccaccacctg
     4921 aacggtgatg tcgaccacga catcggtatg cagcacaacc tgcaccggat gttcgccggt
     4981 catcttgatg gggccgtgcg gcatgcggat ctgcgccttt tccacggcga agccctgctt
     5041 ggtcaccgct tcggcgatgt ctgcattggt caccgaaccg aacagacggc catcaacgcc
     5101 ggccttctgg ccgatctgga ccgaggtacc gcccagcttt tcgccctgcg cctgcgcggc
     5161 agccagcttc tcggctgcgg ccttttccag ttccgcacgc ttggcttcga actcggccac
     5221 agcggtttcg gttgcgcggc gagccttgcg ttgcgggatc aggaagttgc gggcatagcc
     5281 gtccttgacc ttgacgacat cacccaagtt gccgaggttg acgacttttt ccaacagaat
     5341 gacttgcatg tttttctcct atctcgtcgt caatcaggcg ttgtgcaggt cggtgtacgg
     5401 cagcaacgcg aggtagcgcg cacgcttgat tgcggtgtcg acctggcgct gatagtgcgc
     5461 cttggtgccg gtc
//
LOCUS       DATLGF010000011         8577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_293,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000011 DATLGF010000000
VERSION     DATLGF010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 8577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8577
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VM406_00355"
     CDS             <1..333
                     /locus_tag="VM406_00355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL74437.1"
                     /translation="VSEKETELPAELATALFRIFQEALTNVGKHAQATRVAVSLCASD
                     NSVSLEVVDNGRGIASQDRAKPRSFGIRGMTERVNALGGKLAIGPAAGGGTALQVRIP
                     LSESEHST"
     gene            330..980
                     /locus_tag="VM406_00360"
     CDS             330..980
                     /locus_tag="VM406_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL74438.1"
                     /translation="MTASPPIRILIADDHAIVREGLKQILAEAGDMVVTGEADNGHDA
                     IRLARRDEADILLLDISMPDKSGIEVLKQVRKDTPRTAVLMLSMHREDQYAIRSLKAG
                     AAGYMTKQSAPAELVDAIRLVASGRKYISAALAQELARQIGDENREVPPHELLSDREY
                     QTLVMIASGKTVSDIAAELSLSVKTISMYRSRVLQKMKLRHNAELTHYAIKNNLVE"
     gene            1286..2893
                     /locus_tag="VM406_00365"
     CDS             1286..2893
                     /locus_tag="VM406_00365"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVL74439.1"
                     /translation="MTGSNKPEQNPAEIAREAFRRLAARRIAPTPDAYREIYEEIAGA
                     PSRPGAEKVLAELAASLAATSASAATLSAELARSLERRDWNNCTRQLTELVAEQVRRA
                     EQAAEQRAPAPPNRRAQPFPLVDEAPAPAPGAPERRQDPEWRELLARALGVGIVALLR
                     NAPDLAQEAEKLAAFARDPANGIDTVSSRLKQLCFKIELRADDMAEEHDMLLRLFRLL
                     LENVTELVDDDTWLAGQMKAVQQLLAGPVSHAALADATRNLKEVIYKQGLLKRSLAEA
                     QATVKSMMMTFIDRLGAIAASTGDYHRKIDGYAQKINAAKGIAELNLILSDVMQDTRT
                     AQTEALRSRDEMIAARKDAQEAEGKVQQLEAQLEEMSELAMEDALTGSLNRRGLDDVF
                     ERELARAQRRHTPLCIALLDLDDFKRVNDTFGHGAGDGVLVHLVRVIRETLRTMDVIG
                     RFGGEEFLIILPDTALAEAAETIRRLQRELTKRIFMHENAKLLITFSAGVAERIGGED
                     QTSLIRRADGALYKAKAAGKNRVAVAD"
     gene            3314..4555
                     /locus_tag="VM406_00370"
     CDS             3314..4555
                     /locus_tag="VM406_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HVL74440.1"
                     /translation="MAAYINTNIASLNAQRNLTTSQSDLATSLQRLSSGLRINSAKDD
                     AAGLAISERFTTQIRGLNVAARNANDGISLAQTAEGAMTEIGNNLQRIRELAVQSRNA
                     TNSDSDRAALNAEAQQLKAEIDRVASTTAFNGTNLLNGSFTNQAFQVGANQGETISIS
                     SIVDASSDALGTWDSVATANASGASGAFANVTLAEGSGHTYSLSVGGVSILTATAPAD
                     DGDAGTSLTTVVNAASVDTALADSTVRTNLANAGITVSGSAANGDLVFTKTDGAAMNI
                     VVANGAGSGGFAGDDFATGTNAVDNGAESEAMTGFASLNISTAEGADAAILAMDGALS
                     ALNTARAEMGAVQNRFTSVIASLQTTSENLSASRSRIMDADFAAETAQLTRGQILQQA
                     GTAMLAQANSLPNGVLALLRG"
     gene            4631..4990
                     /locus_tag="VM406_00375"
     CDS             4631..4990
                     /locus_tag="VM406_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HVL74441.1"
                     /translation="MDIRPINTAATAPVAISESGTPPAAPKTAAPVQIADPVRAASPA
                     ESAQQLDDAVKSINKALKELTQGVEFTIDSNSDKPIVKVVDHETKEVLRQIPSEEALA
                     ISRALDRVQGLLIRQQA"
     gene            5098..6540
                     /gene="fliD"
                     /locus_tag="VM406_00380"
     CDS             5098..6540
                     /gene="fliD"
                     /locus_tag="VM406_00380"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HVL74442.1"
                     /translation="MPSLSSPGVGSNLDVREIVSQLMAVERQPLKVLDSKEASFKAKL
                     TTLGQIKSALTSLQSAALTLSLSSTYNAVKPSVADSGVMSVAITGATPPGSYNVEVKQ
                     LAQAQKLISGTHAAMDSTVGTGTLKIEVGRYSMPDAPPVGFTPKEGVDAVTITIDDKN
                     NTLSGIRDAINASGAGVTATIINDGSGYRLSLTSKESGEANEVRITASETGDPGLAQL
                     AYDRSEGGISNLHQNVAARDAILKIDGMLVTKPTNTITDAIQGVTLTLTKETSESGTK
                     LTLTHDTSAIRTAIDNFIKAYNEVHKQIGAATNYDATSQTASILTGDATMRSLQSQLR
                     NALSTALPGSPGGMSVLSQVGIAFQRDGSLALDADKLAKAIADPNKDVSQLFIRRENG
                     EPGFGGRIHGLVSKMILGEDALLNGRMDGINNSIKNLDKQRQAQEARMASIEQRYLAQ
                     FTALDVAISRMTSTSSFLTQQLEALKAQTK"
     gene            6554..7036
                     /gene="fliS"
                     /locus_tag="VM406_00385"
     CDS             6554..7036
                     /gene="fliS"
                     /locus_tag="VM406_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HVL74443.1"
                     /translation="MFGSSMSGASAYSKVGMETGVVAASPHKLIVMLFEGAMLSITAA
                     QKHMQAGETEAKGRAIGKAVSIIEEGLRASLDKKVGGEIALSLDSLYEYMVARLTIAN
                     LKNQPAILEEVHQLLKGLKDAWEAIDPARAAQSVQPAPAPAAKAYDPLAPTQSRLVKA
                     "
     gene            7043..7375
                     /locus_tag="VM406_00390"
     CDS             7043..7375
                     /locus_tag="VM406_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HVL74444.1"
                     /translation="MTNEDVLALYESVADITTQMLAAARTGDWEQLAALESRCSRQVE
                     ILKAEEPVAPLPPVQRERKVKIIKKILDDDRQIRDITEPWLAHLSAMISSAGTERKLA
                     KAYGAGQN"
     gene            7563..>8577
                     /locus_tag="VM406_00395"
     CDS             7563..>8577
                     /locus_tag="VM406_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HVL74445.1"
                     /translation="MLPRADLSTNRPPGAVEAPTPATSATDTRQEVFRRLNQIAIGKE
                     LSATVQARLEDGSHLVKIGDAAARMVLPAGTRVGDQFSMVFIAREPRPTFLLNGQAGS
                     ANASLSAAARLIDHLLHGARGDSAAMTVRGSTPLLPGPMMDAAQLAGQLQRALASSGL
                     FYESHLQEWVVGSRSKADLAREPQAAFAQPRQAPAPDGQEIARLAAGLKELGEGAQAL
                     LRMMRDAQRHAGMDADLLARTPAPLPALDPELGRLIQMQLNTLEQQQIRWQGQLFPGQ
                     EMEWDVTEERSQDDPDNHDASAWTSVVRFELPHLGPVEAVLRLAGDRVQAEIRAEKDA
                     SAAA"
BASE COUNT         1771 a         2942 c         2642 g         1222 t
ORIGIN      
        1 gtcagcgaaa aggagaccga gctgcccgcc gaacttgcga ccgcgctgtt tcgcatcttc
       61 caggaggcgc tgacgaatgt cggcaagcat gcgcaggcca cccgcgtcgc cgtcagcctc
      121 tgtgccagcg acaactcggt gagcctggag gtggtggaca atggccgcgg catcgccagc
      181 caggaccgcg ccaagccgcg ctccttcggc atacgcggca tgaccgaacg cgtcaacgcg
      241 ctgggcggca agctggcgat cggcccggcc gccggcggcg gcaccgcgct gcaggtccgc
      301 atacccctat ccgaatccga acacagcaca tgacagcaag ccctcccatc cgcatcctga
      361 tcgccgacga ccatgcgatc gtgcgcgaag gactcaagca gatcctcgcc gaggccggcg
      421 acatggtcgt gaccggcgag gccgacaacg gccacgatgc gatccggctc gcgcggcgcg
      481 acgaggccga catcctgctg ctcgacatct cgatgccgga caagagcggc atcgaagtgt
      541 tgaagcaggt gcgcaaggac acgccgcgca ccgccgtgct gatgctgtcg atgcaccgcg
      601 aggaccagta cgcgatccgc tcgctgaagg ccggcgcggc cggctacatg accaagcaga
      661 gcgcaccggc ggaactggtg gacgcgatcc gcctggtcgc ctccggacgc aagtacatca
      721 gcgccgccct cgcccaggaa ctggcacggc agatcggcga cgagaaccgc gaggtgccgc
      781 cgcacgaact cctctccgac cgcgaatacc agacgctggt gatgatcgcc tccggcaaga
      841 cggtcagcga catcgccgcc gagctgtcgc tgtcggtcaa gaccatcagc atgtaccgct
      901 cgcgcgtgct gcagaaaatg aagctgcggc acaacgcgga gctgacgcac tacgcgatca
      961 agaacaacct ggttgagtag gtttgccctc gcgcatatgc gacagggtgt gaggtgccat
     1021 gcttgacccc ggctcaaact caccatgtcg ttcccatagg cgcccccgcg caggcgggaa
     1081 cccagctccg tcaccgcagc accacagaag ccccgaacca cactcccgct ccgccaaagc
     1141 cacctgcccc agcgctccca cccgccaccc atccgcgaat tgcccatcat tccccccgct
     1201 gcttgccgaa ttgccagcct gtcatcgcga ttagcattca agaataccta gacgaatccg
     1261 cccgctgccg ggcccagatg ccgccatgac aggctcgaac aaaccagaac aaaatcccgc
     1321 cgagatcgcg cgggaagcct ttcgccgcct tgcggcgcgg cgcatcgcac ccacccccga
     1381 cgcctatcgc gaaatctacg aagaaattgc cggcgccccc tcgcgccccg gcgcggaaaa
     1441 agtgctggcc gaactcgccg ccagcctcgc cgccacttcg gcttcagcag ctacgctctc
     1501 ggccgagctc gcccgctcgc tcgagcggcg cgactggaac aattgcacgc gccagctgac
     1561 tgaactggtg gccgaacagg tacgacgggc ggagcaggcg gccgaacaac gcgcccccgc
     1621 gccgccaaac cggcgcgcgc aaccgtttcc gctggtggac gaggcgcccg cccctgcccc
     1681 cggcgcgccg gagcgcaggc aggacccgga atggcgcgaa ctgctggcgc gcgcactcgg
     1741 ggtgggcatc gtcgcgctgc tgcgcaacgc acccgatctg gcccaggaag ccgaaaagct
     1801 cgccgcgttc gcacgcgatc cggccaacgg catcgatacg gtgtccagcc ggctcaagca
     1861 gctgtgcttc aagatcgagc tgcgcgccga tgacatggcc gaggagcacg atatgctgct
     1921 gcggctgttc cgcctgctgc tggaaaacgt caccgagctc gtggacgacg acacctggct
     1981 ggcgggacag atgaaggcgg tccagcagct gcttgccgga cctgtcagcc atgccgcact
     2041 ggccgacgct acgcgcaacc tgaaggaagt catctacaag cagggcctgc tcaagcgcag
     2101 cctggccgaa gcccaggcga cggtaaaaag catgatgatg accttcatcg accgcctcgg
     2161 tgcgatcgcc gccagcaccg gcgactacca ccgcaagatc gacggctatg cgcagaagat
     2221 caatgcagcc aagggcatcg ccgaactcaa tctcatcctc tccgacgtga tgcaggacac
     2281 ccgtaccgcg cagaccgagg cgctgcgctc gcgcgacgag atgatcgccg cgcgcaagga
     2341 cgcgcaggaa gcggaaggca aggtccagca gctcgaggcg caactggaag aaatgagcga
     2401 gctggcgatg gaggacgcgc tgaccggcag cctgaaccgg cggggactcg acgacgtctt
     2461 cgaacgcgaa ctcgcgcgtg cacagcggcg ccacaccccg ctgtgcatcg ccctgctcga
     2521 cctcgacgac ttcaagcgcg tcaacgacac cttcggccat ggcgcgggcg acggcgtgct
     2581 cgtgcacctg gtgcgcgtga tccgcgagac gctgcgcacg atggacgtga tcggccgctt
     2641 cggcggcgaa gaattcctga tcatcctgcc ggacaccgcg ctcgccgaag cggccgagac
     2701 aatccgtcgc ctgcagcgcg aactcaccaa gcgcatcttc atgcacgaaa acgcgaagct
     2761 cctgatcacg ttcagcgccg gcgtggccga gcgcatcggc ggcgaagatc agaccagcct
     2821 gatccggcgc gccgacggcg cgctgtacaa ggccaaggcg gcgggcaaga accgggtcgc
     2881 cgtggcggac tgaggccgtt ctcgcctcac attctttcaa ttctcgcaac acgaagccag
     2941 accgggacgc ggttacggca gccgccgcca aaactttagg ccctccacca tcaccttcga
     3001 taacgcggcg cgcccgcggg caccacttca tggcgaaaac ccgcgccagc tctcgctttc
     3061 cgggccttgg acacccccgc gcgcgccgca aaaaaaattt cccccttaaa gaattttgtt
     3121 tcctcaccga aacagcaggt gtccggcttg gaaacggaca ggctgaaaaa aaccggaaaa
     3181 caccccctaa acttttttca gactcgtccg ttaaacgatt catcagcggt tcaaacggca
     3241 gcgaaagcgc ggtgggaccg gcaaggacgc caaggcggca tcgaacgatc gcagtttaaa
     3301 caggagaatc aacatggctg catatatcaa caccaacatc gcttcgctga acgcgcagcg
     3361 caacctgacc acgtcgcaga gcgatctcgc gacctcgctg cagcgcctgt cctcgggcct
     3421 gcgcatcaac agcgccaagg acgacgctgc cggcctggcg atctccgagc gcttcaccac
     3481 ccagatccgc ggcctgaacg tcgccgcccg caacgccaac gacggcatct ccctggcgca
     3541 aaccgccgaa ggcgcgatga ccgaaatcgg caacaacctg cagcgtatcc gcgaactggc
     3601 tgtgcagtcg cgcaacgcca cgaactcgga ttccgaccgc gccgcgctga acgccgaagc
     3661 ccagcagctg aaggcggaaa tcgaccgcgt cgcctcgacc accgcattca acgggacgaa
     3721 tctgctcaac ggcagcttca ccaaccaggc attccaggtt ggcgcgaacc agggtgaaac
     3781 tatcagcatc agcagcatcg tcgatgcgtc ttccgacgcc ctcggaactt gggattcggt
     3841 cgcaacggcg aacgccagcg gcgcatcggg cgcatttgcc aacgtgacgt tggcggaagg
     3901 ctctgggcac acgtattcgc tcagcgtcgg cggcgtctcg attcttacag caacggctcc
     3961 tgccgacgat ggcgacgcgg gcacaagcct caccaccgtc gtcaatgcag caagcgttga
     4021 taccgccctg gccgactcca cggtgcgaac aaacctggcc aacgccggca ttaccgtctc
     4081 cggtagcgct gcgaatggcg acctggtctt cacgaagacg gatggtgctg caatgaacat
     4141 cgttgtagcg aatggtgcgg gctccggcgg attcgcaggt gacgatttcg ctaccggtac
     4201 taacgcagtc gacaatggcg cggaatctga agcgatgacc ggcttcgcaa gcctgaacat
     4261 ctcgaccgcc gaaggcgctg acgcagcaat cctggcgatg gacggcgcgc tcagcgcact
     4321 gaacaccgcc cgcgccgaaa tgggtgcagt gcaaaaccgc ttcacctcgg tgatcgccag
     4381 cctgcagacc acgtcggaaa acctgtccgc atcgcgcagc cgcatcatgg atgccgactt
     4441 cgccgcagaa accgcgcagc tgacccgcgg ccagatcctg cagcaagccg gtaccgcaat
     4501 gctggcccag gccaactcgc tgccgaacgg cgtcctggcc ctgctgcgcg gctaagcagc
     4561 accagcctga tgtagcgacg cccggccggg gctttcccag gccgggcttt tctccttctc
     4621 ggagcacgtc atggacatac gacccatcaa cacggcagcg accgccccgg tcgccatcag
     4681 cgaaagcggc acgccgcccg ccgcgccgaa aaccgccgcc ccggtccaga ttgccgaccc
     4741 ggtgcgcgcc gcctcgccgg ccgaatcggc gcaacagctc gacgatgcgg tgaagtcgat
     4801 caacaaggcc ctgaaggaac tgacgcaagg cgtggaattc acgatcgaca gcaacagcga
     4861 caagcccatc gtcaaggttg tcgaccatga aacgaaggaa gtgctgcgcc agattccgag
     4921 cgaggaagcg ctggcgatct ccagggcgct ggaccgcgtg caaggcttgc tgatccgcca
     4981 acaggcttga tggcgggcgc cccgggaacg caggaatagg cgggaaaatc gccacctaaa
     5041 gcccgcttcg gcgctgccga tagcaaagta tattgatgct ttaattagga gactctagtg
     5101 ccctcactca gctctcccgg cgtcggctcc aacctggatg taagggaaat cgtctcccag
     5161 ttgatggcag tcgagcgcca gccattgaag gtcctggaca gcaaggaagc ctcgttcaag
     5221 gccaagctga cgacgctcgg ccagatcaag agcgcgttga cgtcgctgca gagcgcggcg
     5281 ctgacgctga gtctctcctc gacctacaac gcggtcaagc ccagtgtcgc ggactccggc
     5341 gtgatgagcg tggcgatcac cggcgccacc ccgcccggct cctacaacgt cgaggtcaag
     5401 cagctcgcgc aggcgcagaa gctgatttcc ggcacccatg ccgcgatgga ttccaccgtc
     5461 gggaccggca cgctgaagat cgaagtcggt cgctattcga tgcccgatgc accgccggtc
     5521 ggcttcacgc cgaaggaagg cgtggacgcc gtcacgatca cgatcgacga caagaacaac
     5581 acgctctccg gcatccgcga tgcgatcaac gcatccggcg ccggcgtcac cgccaccatc
     5641 atcaacgacg gatccggcta tcggctctcg ctgacatcga aggagtccgg cgaggcaaac
     5701 gaggtgcgga ttaccgcgag tgaaaccggg gatcccggcc ttgcccagct tgcctatgac
     5761 cgcagcgagg gcggaatctc gaatctgcat cagaacgtcg ccgcgcgcga cgcgatcctg
     5821 aagatcgacg gcatgctggt caccaagccg accaacacga tcaccgatgc gatccagggc
     5881 gtgacactca cgctcaccaa ggaaaccagc gagagcggca ccaagctgac gctcacccat
     5941 gacacgtcgg caatccgcac cgcgatcgac aacttcatca aggcctacaa cgaggttcac
     6001 aagcagatcg gcgcggcaac caactacgat gccacgagcc agacggcaag catcctgacc
     6061 ggcgacgcca cgatgcgctc gctgcagtcg cagctgcgca atgccctctc gacggcgctg
     6121 cccggctcgc ccggcggcat gtcggtgctg tcccaggtcg gcattgcatt ccagcgcgac
     6181 ggcagcctcg cgcttgatgc ggacaagctc gccaaggcca tcgccgatcc caacaaggat
     6241 gtcagccagc tgttcatccg tcgcgagaac ggtgaacccg gcttcggcgg ccgcatccac
     6301 ggtctggtca gcaagatgat cctgggcgag gatgcgctgc tcaacggccg catggacggc
     6361 atcaacaact cgatcaagaa cctcgacaag caaagacagg cgcaggaagc gcggatggcg
     6421 tcgatcgaac agcgctatct tgcccagttt accgcgctcg acgtggcaat ctcgagaatg
     6481 acctcgacca gcagcttcct cacgcagcag ctcgaggcgc tgaaggcaca gacgaaatag
     6541 caggagactc atcatgtttg gttcatcaat gagcggcgcc agcgcctaca gcaaggtcgg
     6601 catggaaacc ggcgttgtcg ccgcgagccc gcacaagctc atcgtcatgc tgttcgaggg
     6661 cgcgatgctg tcgattacag cggcgcagaa gcacatgcag gccggcgaga ccgaggccaa
     6721 gggccgcgcg atcggcaagg ccgtgtcgat catcgaggaa ggcctgcgcg cgagcctgga
     6781 caagaaggtc ggcggcgaga tcgcgctcag ccttgattcc ctctacgaat acatggtcgc
     6841 gcggctgacc atcgccaacc tgaagaacca gccggccatc ctggaagagg tccatcagct
     6901 gctcaagggc ctgaaggacg catgggaggc gatcgacccg gccagggccg cgcagagtgt
     6961 ccagcccgcg ccggctccgg cggcgaaggc ctacgacccg ctcgccccca ctcaatcccg
     7021 actcgtgaag gcttgaatga caatgactaa cgaagacgtt ctcgcgctgt acgaatcggt
     7081 tgcggacatc accacccaga tgctggctgc ggcgcgcacc ggcgactggg agcagcttgc
     7141 cgcgctggaa tcgcgctgca gccgccaggt cgagatattg aaggccgagg aaccggtcgc
     7201 tccgctgccg ccggtgcagc gcgagcgcaa ggtgaagatc atcaagaaga tcctggacga
     7261 cgaccgccag atccgtgaca tcaccgaacc ctggctggcc catttgtcgg ccatgatcag
     7321 cagcgccggc acggagcgca agctcgcgaa agcctacggc gcgggccaga actagtgtcc
     7381 tgtcccgtaa ataggtgcgc aaaatgaaat cgatgcattt cgggtcagga caaggcctat
     7441 gccggccgcg agcaaaccgc agcgataccg ggtggtatcg cgaggatttg cgacgcaggc
     7501 atggcccgaa aggcgcgatt tcatgcgcac ttatttgcgg gacaggacac taggcccccg
     7561 ccatgctgcc gcgggccgac ctcagcacca accgcccgcc tggcgcggtc gaggcgccga
     7621 cgcccgcgac ctccgccaca gatacccggc aggaagtctt ccggcggctg aaccagattg
     7681 cgatcggcaa ggagctgagc gcgacggtgc aggcgcgcct cgaggacggc agccacttgg
     7741 tgaagatcgg cgatgccgcc gcgcgcatgg tcctgcccgc ggggaccagg gtcggcgacc
     7801 agttttccat ggtcttcatc gcacgcgagc cgcgccccac tttcctgctg aacggccagg
     7861 ccggcagcgc caatgcgtca ctgtccgccg ccgctcgcct gatcgaccat ctgctccatg
     7921 gtgcgcgcgg cgacagcgcc gcgatgacgg tgcgcggcag cacgccgctc ttgcccgggc
     7981 cgatgatgga tgccgcacag ttggccggcc agttgcagcg cgcgctggca tccagcggcc
     8041 tgttctacga atcgcatctg caggaatggg tcgtcggcag ccgcagcaag gcggatctgg
     8101 cgcgcgagcc gcaggccgcg ttcgcccagc cgcgccaggc gcctgcgccg gacggacagg
     8161 agatcgcgcg gctggccgcg ggattgaagg aactcggcga aggcgcgcag gccctgttgc
     8221 gcatgatgcg cgatgcgcag cgccatgcgg gcatggacgc ggacctgctc gcgcgcacgc
     8281 cggcgccgct gcccgcgctt gatcctgagc tgggacggct gatccagatg cagctcaata
     8341 cgctggagca gcagcagatc cgctggcagg gccagctgtt tcccggtcag gagatggaat
     8401 gggatgtcac cgaggagcgc tcgcaggatg atccggacaa tcacgacgcg tccgcctgga
     8461 ccagcgtcgt gcgcttcgag ctgccgcatc tcggcccggt cgaggccgtg ctgcggctgg
     8521 cgggcgaccg cgtgcaggcg gagatccgcg ccgagaagga cgccagcgcc gccgcac
//
LOCUS       DATLGF010000012         4417 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_174533, whole genome shotgun sequence.
ACCESSION   DATLGF010000012 DATLGF010000000
VERSION     DATLGF010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174533"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VM406_00400"
     CDS             <1..220
                     /locus_tag="VM406_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HVL74446.1"
                     /translation="EKDMYQLTVEVMSCGHCIASVTKAVQTLDAGAKVDVELATQTVR
                     VDTRAALDDVRRAVADAGFPVHQAQAQG"
     gene            complement(235..621)
                     /gene="cueR"
                     /locus_tag="VM406_00405"
     CDS             complement(235..621)
                     /gene="cueR"
                     /locus_tag="VM406_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - intracellular copper ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HVL74447.1"
                     /translation="MNIGQAARESGVSAKMIRYYESIDLIAPGRRTEAGYRVYGENDV
                     HTLRFIKRARTLGFSLDQIRQLLSLWRDPHRASGDVKRIAEVHVEELDQRIREMTEMR
                     DTLARLATACCGDSRPECPILESLSE"
     gene            complement(618..3026)
                     /locus_tag="VM406_00410"
     CDS             complement(618..3026)
                     /locus_tag="VM406_00410"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HVL74448.1"
                     /translation="MQSPSLALPAEEARTFQLRIEGMSCASCVARVEKALRAAPGVIE
                     ASVNLATETASVTALPEVAPASLGAVVEKAGYHVAEDEIDLDIEGMSCASCVGRVERA
                     LRKVDGVLEASVNLATETARVRVAAGTPVDALLGAAEQAGYPARLHHAEPGSAPVVHG
                     DRERQHVIFAVLLSLPLAAPMIGQPFGYDWMLPGWLQWLLATPVQFWLGARFYRAGWA
                     ALRAGTGNMDLLVALGTSAAYGLSVYLLLEHGEHGMPHLYFEAAAVGITLVLLGKWLE
                     ARAKRQTTDAIRALQQLRPEVARVRDGVVEREVPIARVRTGDLVVVRPGERIAVDGEV
                     VEGISHVNESMITGESLPVARGPGERVTGGSINAEGLLLVRTTAVGAESTLARIIRLV
                     ENAQAAKAPIQRLVDKVSAVFVPVVLVIAAASLLGWSLAGADIETAILNAVSVLVIAC
                     PCALGLATPTAIMAGTGVGARAGILIKDAEALEQAHGVTAVVFDKTGTLTVGQPALVA
                     AQPNGIDDKTLLELAASVQQGSEHPLARAVLEAAQRQGVSPRPAADVAALPGRGMQAR
                     VGTDVLHLGSTRLMQEQRVDLEPLLARARELEGQGNTVSWLASGERLLGLLAFGDRIK
                     PGAERAVGRLRELGVKTVLLTGDNRGSAQAVGGALGIDEVVAEVLPADKAAKVVALKE
                     AGHVVAMVGDGINDAPALAAADVGVAMGTGTDVAMHAAGVTLMRGDPVLVADAIDVSR
                     RTYRKIRQNLFWAFIYNVIGLPLAAFGLLNPVLAGAAMAFSSVSVVANALLLRRWKPA
                     ERNV"
     gene            complement(3081..3950)
                     /locus_tag="VM406_00415"
     CDS             complement(3081..3950)
                     /locus_tag="VM406_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HVL74449.1"
                     /translation="MPRLLIVGCGDVGMRMLPLLRGRFRVFAVTSQEARAAELRAAGA
                     VPIVADLDRPHTLARLARLATAIVHLAPPQSQGQKDLRTRRLAAILPDRATLVYVSTS
                     GVYGDCGGEWVQETRPVRPQSARAQRRVDAEQVLRRWARRSGSRLAILRVPGIYAEDR
                     LPVERLKRATPALRDEDDVYTNHIHADDLARIVVTALFRARPCRIYHASDDSEMKMGA
                     YFDAVADAVDLPRAPRLPRAELERAVSPLLLSFMSESRRLANDRLKAELGVRLKCARV
                     ADALARMETRQAA"
     gene            4017..>4417
                     /locus_tag="VM406_00420"
     CDS             4017..>4417
                     /locus_tag="VM406_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HVL74450.1"
                     /translation="MTFQVTVQPSGRQFTCEESETVLTAAIRNGVGLPYGCKNGACGT
                     CKGKLLEGSVTHGAHQEKALSVAEEEKGLALFCCATAHSDLVIEAREVLGVGEIPIKK
                     MPTRVAKLDRVADDVMVVSLQLPANERLQYKA"
BASE COUNT          713 a         1607 c         1421 g          676 t
ORIGIN      
        1 ggagaaggat atgtaccagt tgacggtgga agtcatgagc tgcggacact gcatcgcctc
       61 ggtgacgaag gcggtgcaga cgctggacgc aggcgcgaag gtcgatgtgg agctggcaac
      121 gcagacggtg cgcgtggaca cccgcgccgc actcgacgac gtgcggcgcg cggtcgccga
      181 cgccggcttt cccgtgcatc aggcgcaggc acagggatag ccggcgtcgc ggcactactc
      241 cgacagactc tccaggatag gacactcggg ccggctgtcg ccgcagcaag ctgtcgccag
      301 cctggccagc gtgtcgcgca tctcggtcat ttcccggatc cgctggtcca gttcctcgac
      361 gtgcacttcg gcgatgcgct tgacgtcgcc gctggcgcgg tgcggatcgc gccacagcga
      421 cagcagctgc cggatctggt ccagtgaaaa gcccagcgtg cgggcgcgct tgatgaagcg
      481 cagcgtgtgc acgtcgttct cgccgtagac gcggtaaccg gcttcggtgc gccgcccggg
      541 cgcaatcagg tcgatgctct cgtagtagcg gatcatcttc gcgctcacgc ccgattcgcg
      601 ggccgcctgt ccgatattca tacgttcctt tccgccggct tccagcgccg caacagcaac
      661 gcattggcaa ccacgctgac cgagctgaac gccatcgcgg ccccggccag caccgggttg
      721 agcaggccga acgcggccag cggcagaccg atcacgttgt agatgaaggc ccagaacagg
      781 ttctggcgga tcttgcgata cgtgcgccgc gacacatcga tcgcgtcggc gacgagtacc
      841 gggtcaccgc gcatcaacgt cacgccggcc gcatgcatcg ccacatcggt gccggttccc
      901 atcgccacgc cgacatcggc cgcggccaga gccggtgcgt cattgatgcc atcgccgacc
      961 atcgccacca cgtgtccggc ctccttcagc gccacgacct tcgccgcctt gtcggcgggc
     1021 agcacctccg ccacgacctc gtcgatgccc agcgcgccgc caaccgcctg cgcgctgccc
     1081 cgattgtcgc cggtcaacag aaccgtcttc acgccgagtt cgcgcagccg tcccaccgct
     1141 cgctcggccc ccggcttgat ccggtcgccg aaagcgagca ggcccaagag ccgctcgccg
     1201 ctcgccagcc aggagacggt attgccctgc ccttccagct cgcgggcgcg cgccagcaag
     1261 ggttcgagat ccacgcgctg ctcctgcatc agccgcgtgc tgccgagatg cagcacgtct
     1321 gtgcccacgc gtgcctgcat tccacggccc ggcaacgcgg ccacatccgc cgccggcctg
     1381 ggcgacacgc cctgccgctg cgccgcttcc agcaccgcgc gcgccagcgg gtgctcgctg
     1441 ccctgctgta ccgacgccgc cagttccagc agggtcttgt cgtcgatgcc gttcggctgc
     1501 gcggccacca gcgccggctg gccgaccgtc agcgtgccgg tcttgtcgaa caccacggcg
     1561 gtgaccccat gcgcctgctc cagcgcctcg gcatccttga tcaaaatgcc ggcgcgcgcg
     1621 ccgacgccgg tgccggccat gatcgcggtc ggcgtcgcca gtccgagcgc gcacggacag
     1681 gcgatcacca gcaccgacac cgcgttcagg atcgcggttt cgatgtccgc ccccgccagc
     1741 gaccagccca gcagcgaagc cgccgcgatc accagtacga ccggcacgaa caccgcgctc
     1801 accttgtcca ccagccgctg gatcggcgcc ttggccgcct gggcgttctc caccaggcgg
     1861 atgatgcgcg ccagcgtcga ttcggcgccg accgccgtcg tgcgcaccag cagcaggcct
     1921 tccgcgttga tcgatccgcc ggtcacccgc tcgcccggtc cgcgcgccac cggcaggctt
     1981 tcgccggtaa tcatcgattc gttcacatgg ctgatgccct cgaccacctc gccgtcgact
     2041 gcgatgcgct cgcccggccg caccacgacg aggtcgccag tacgcacgcg ggcaatcggg
     2101 acttcgcgct cgacgacacc gtcgcgcacg cgcgcgacct cgggccgaag ctgctgcagc
     2161 gcacggatcg catccgtggt ctggcgcttg gcgcgcgcct ccagccattt gccaagcagc
     2221 accagggtga tcccgaccgc ggccgcttcg aaatacagat gaggcatccc gtgctccccg
     2281 tgttcgagca gcaggtagac agacaaacca tatgccgcgc tggtgccgag cgcgaccagc
     2341 aggtccatgt tgccggttcc ggcgcgcagc gccgcccagc cggcacggta gaagcgcgcg
     2401 ccgagccaga actgcaccgg cgtggccagc agccactgca gccagccggg cagcatccag
     2461 tcgtagccga acggctggcc gatcatcggc gcggccagcg gcagcgaaag cagtaccgcg
     2521 aagatcacgt gctggcgctc gcggtcgcca tgcacgaccg gtgctgagcc gggctcggca
     2581 tgatgcaggc gcgccggata gccggcttgt tcggcggcgc ccagcagcgc gtcgaccggc
     2641 gtgccggccg ccacacgcac gcgcgcggtt tcggtcgcca ggttcacgct ggcctctagc
     2701 acgccatcaa ccttgcgtag cgcccgttcc acccggccca cgcaggacgc gcagctcatg
     2761 ccttcgatgt cgaggtcgat ctcgtcctcg gccacgtgat aaccggcctt ctcgaccacc
     2821 gcgccaagac tcgccggcgc gacctcgggc agcgcggtga cgctcgcagt ctcggtggcc
     2881 agattcaccg acgcctcgat cacgccgggc gcggcgcgca aggccttctc cacgcgcgcg
     2941 acgcacgacg cgcagctcat gccctcgatt ctcaactgga aagtccgcgc ttcctcggcg
     3001 ggaagcgcca agcttgggga ttgcatgttg gctccatcgg tcaggatggg ctcattgtcc
     3061 tccttcccat gatgggaagg tcaagcggct tgtcgcgttt ccattctggc aagcgcatcc
     3121 gccacgcgcg cgcacttcag gcgcacgccc agttcggcct tgagccggtc gttggcaagc
     3181 cggcgcgact cggacatgaa agaaagcaac agcggtgaga ccgcacgctc gagttccgca
     3241 cgcggcaggc gcggcgcgcg cggcaggtcc actgcatcgg ccaccgcatc gaagtaggcg
     3301 cccatcttca tttcgctgtc gtcgctggcg tggtagatgc ggcagggccg cgcgcgaaac
     3361 agcgccgtca ccacgatgcg ggcgaggtcg tcggcgtgga tgtgattggt atagacgtca
     3421 tcctcatcgc gcagcgccgg cgtcgcgcgc ttcaggcgtt ccacaggaag ccggtcttcc
     3481 gcatagatgc cgggcacgcg caggatggcc aggcgcgagc cggagcgccg cgcccagcgc
     3541 cgcagtacct gctcggcatc gacacgccgc tgcgcgcggg cgctttgcgg acgcaccggc
     3601 cgggtttcct gcacccactc gccgccgcag tccccgtaca ccccgctcgt gctcacataa
     3661 acgagggtgg cccggtcggg taaaatggcg gccagtctgc gggtacgcag gtccttctgg
     3721 ccctgcgact gcggtggcgc gagatggacg atcgccgtcg ccaggcgcgc gagccgcgcc
     3781 aacgtgtggg gccgatcgag gtcggccacg atcggcaccg cgccggcggc gcgcagttcg
     3841 gctgcacgtg cttcctggct ggtcacggcg aatacgcgaa agcgcccgcg cagcagcggc
     3901 aacatgcgca tgcccacatc gccgcagccg acgatcaaca ggcgcggcat cgcaagacgc
     3961 cgccgcgctc cgttcccgct tccattgtct tcacattttt tcatcgtcgc gattgtatga
     4021 ctttccaagt taccgttcag cccagtggcc gccagttcac ctgcgaagaa agcgaaaccg
     4081 tcctcacggc cgcaatcaga aatggcgtcg gccttccgta tggttgcaag aacggcgcct
     4141 gcggcacctg caagggcaag ctgctggagg gttcggtcac ccacggcgcc caccaggaaa
     4201 aggcgctgtc ggtcgccgag gaagaaaagg gactcgcgct gttctgctgc gccaccgcgc
     4261 attcggacct ggtgatcgag gcgcgtgaag tgctgggcgt gggcgagatc ccgatcaaga
     4321 agatgccgac ccgcgtcgcg aagctcgacc gcgtcgccga cgacgtgatg gtcgtctcgc
     4381 tgcagctgcc cgccaacgag cggctgcaat acaaggc
//
LOCUS       DATLGF010000013         6029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_127489, whole genome shotgun sequence.
ACCESSION   DATLGF010000013 DATLGF010000000
VERSION     DATLGF010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127489"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..1175)
                     /locus_tag="VM406_00425"
     CDS             complement(210..1175)
                     /locus_tag="VM406_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74451.1"
                     /translation="MPTFTGGSMKKLPIAILVSLLGASGAAFAAGGTTGTTGDTGTTG
                     ATATSPVAGTIQEGRADAQDTAAGATTGDTTTGTTAAGTTDTAGTMGTTGETGTAGAA
                     ATGTDTTGTTGTTGTTGMTGTDTTGATGTTGMTGTGTTGATGTDATGATGAGATAGTA
                     TDATGATGTAGTVGTAPALCPCPPGVTGAPGTTGETGTGTDATGATGTTGTMGTGTEP
                     ATGGVTDTTGTTGTDATTGTGTGTGTTGDTGVTGTTGTGTTDTPGATGTDTTTGTTGD
                     TGTTGTTGAGATDTTGTTDTTGTTGTTGATGVYTGPGAAGTGTSR"
     gene            1473..2915
                     /locus_tag="VM406_00430"
     CDS             1473..2915
                     /locus_tag="VM406_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HVL74452.1"
                     /translation="MSEKHAAPPGTPSPASASGRPQPVIALREAIALIVGIVIGAGIF
                     AAPAMVAGMTGSAPWMFGAWLLGGLMSLIGALCYAELATAYSHAGGDYHFLRRAYGRS
                     VAFLFGWARFSVITTGSIALLGFVFGDYMNQVWPIGGFGILSGPTLYAAVSIVILSFI
                     NMRSTMSGTYTQTWLTALEVGGLLIIVAAALYLAGTGTPPATLPAADAPAGVPSAAAF
                     GLAMVFVLLTFGGWNEAAYISAELRDGRRGIVKALIWSIVIITAVYMLVIWAYWRALG
                     IEGMANSQAMAADVLGVAFGPVGAMSISLMVAVAALTSMNATMIVGARTNYAVGQDWH
                     SLKLLSHWDDERDAPLNALRVQTLAALLLVLVGAWTGAGFRSMVEFTAPVFWLFFLLS
                     GVSLFVLRYREPNTERPFKVPLYPLLPLVFCAMCAYMLWSSLSYVYSQSLGGLNAAWI
                     GVGVLAIGLVLLLLVRAQHARQQRSAVKTF"
     gene            2998..3531
                     /locus_tag="VM406_00435"
     CDS             2998..3531
                     /locus_tag="VM406_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVL74453.1"
                     /translation="MLLGSGPAHAQSTTEPVRPPDVPYVPTPQPVVDKMLEMARVGKD
                     DVVYDLGCGDGRIVITAASEFGARGVGIDINPVRIAEAKDNAQKAGVTDKVEFKVGDL
                     FESDFSNATVVTLYLLPEINRKLRPQLWRQLKVGTRVVSHDFDMGDEWPPEKVEQVGY
                     KTIYYWTITDANKKAVK"
     gene            complement(3566..>6029)
                     /locus_tag="VM406_00440"
     CDS             complement(3566..>6029)
                     /locus_tag="VM406_00440"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVL74454.1"
                     /translation="QRELLVVEDAYTDPRFADNPLVRQDPGIRFYAGAPLVTADGHAI
                     GSLGVMDRVARGLSPLQEQALRVLAHEVVAQIELRSKLEETRALLAERDQAQQALERS
                     HMQLEREVQARTEDLQQANRALNASAQRAVREALLSQAIIDSLPGLVYVLDCKGRFLR
                     WNRNYESIAGYSADEMVHARVEDFFPDPDQREQVMQALHGVLAGASNSVEADFLTRSG
                     ERIPYFFTGAPIELDGRAGVCGMGVDISARRRAEEALRLRDRAVQASVNAIIITDLAS
                     NITFVNPAFERITGYTMNEALGRNCRFLQAGDRDQPALATLRAAVDRREECAVLLRNY
                     RKDGTLFWNDVHIAPVRDPHGEVTHFVGVINDMTAIKRYEQELERLINVDALTTLASR
                     KVLHDRLRAAIATAHRLGTTVVVGFIDLDNFKFINESLGHTIGDALLKSVATRLLSCL
                     QGEDTVARYGGDEFAFILVGYNDETQVGMLVDKILKTIDQPFYVEEHQFFVSCSIGLS
                     LYPRDGDDADTLLKNADAAMHLAKERGRNNFQFFTPAMSRRVTERLSMEASLRRALAN
                     DEFELHYQPKLDLRTSQVVGAEALLRWFPRHGRGPVSIPPSAFIPLAEETGLINAIGE
                     WVLHTACAHNKALQARGLPPLRMAVNISARQFEPKTIVSLVQRALSASGLAAASLELE
                     LTESLVMRNPEEAIRVLGELKEMGLCLAIDDFGTGYSSLSQLQRFPVDLLKIDQSFVR
                     DIGADPNDAIIARAVISLGHSLGLDVIAEGVSSQEQLTFLRENGCDEIQGYLFSPPLP
                     MEDLVRFIQEPRPPGNVVPKGA"
BASE COUNT          904 a         1995 c         1978 g         1152 t
ORIGIN      
        1 gttcggccaa tgcggtccag ccgccgatgt tgtagctcag gctctgcatg tttgatgttc
       61 tcccgttggc gtgccggtgg cacgcgccgt tacgggaatt ggacccgaac ccgtgaagtt
      121 ccgcgatgac ggttgtcaat agaaaaacac ccgcgcgggc gtctgccccg tgcgggtgtc
      181 aatgcagcta ctggtctagc ggaaaaaact taacggctgg taccggttcc cgctgcgccc
      241 gggccggtgt agacaccggt tgccccggtc gtaccggtgg tgccggtcgt gtccgtcgtt
      301 ccagtcgtgt ccgtcgcgcc ggcgccggtc gtaccggtcg tgccggtgtc gccggtcgtg
      361 cccgtggtcg tgtcggttcc ggtggcgcct ggcgtgtccg tcgtgccggt gcccgtcgtg
      421 ccggttacgc ccgtgtcgcc tgtcgtgccg gtgccggtgc cggtgccggt agtcgcgtcg
      481 gtgccggtcg tgccggtcgt gtcagtgaca cccccggtag cgggctccgt tccggtgccc
      541 atcgtgccag tcgtcccggt agcgccggtg gcatcggtgc ccgtcccggt ttcaccggtc
      601 gtacccggcg cgccggtcac acccggcggg cagggacaca gtgcaggggc agtgcccacg
      661 gtgccggcgg taccggtggc accggtcgcg tcggtggcag ttccggcggt ggcaccggcg
      721 ccggttgcgc ccgtcgcatc tgtgccggtt gcacccgtcg tgccggtgcc ggtcatgccg
      781 gtcgtgccgg ttgcgcccgt cgtgtcagtg ccggtcatgc cagtcgtgcc ggttgtgccg
      841 gttgtgccgg tcgtatcggt ccccgtggcg gcggcacctg cggttccggt ctcgcccgtc
      901 gtacccatgg taccggcggt atcggtcgtg cccgccgcgg tcgtgccggt tgtggtgtca
      961 ccagtggtgg cgccggcagc cgtgtcctgc gcgtctgcgc gtccctcctg gatggtgccc
     1021 gcaacaggac tggtcgccgt tgcgccggtg gtgccggtat cacccgtcgt cccggttgtg
     1081 ccgccggcgg caaacgcagc cccgctcgcg cccaggagcg aaacgaggat cgctatcggt
     1141 agttttttca tgcttcctcc agtaaaagtt ggcaagtgaa tgtcctcggc catgccgaag
     1201 gtagtcgtta gacaaacatc aacccgctgg ttcgcgtaat cgcacgctct cttgtgctgc
     1261 agcgccccgg agcgaggcgc ggccgtgcgc gtatttgatg ggccgcaacc gccttgcagc
     1321 tgcgcaagca cgacgcgcca cttctggtgc acgatgcgag cggtttgcgc tgcgcgtagc
     1381 ggtttgttac gatcggttcc tgcattcggc cgccaacaag aacaacgagg cgcgtcgccc
     1441 gcccccctcc attccacttc cgatgagtca gcatgagcga aaaacacgca gcgccccctg
     1501 gtactccgtc accggcctcg gcttccggcc gcccccaacc tgtcatcgca ctgcgtgaag
     1561 cgattgcgct gatcgtcggc atcgtgatcg gcgccggcat cttcgctgct ccggcaatgg
     1621 tggccggcat gaccggcagc gcaccgtgga tgttcggcgc ctggctgctc ggcggcctga
     1681 tgtcgctgat cggcgcgctg tgctacgccg agctcgcgac cgcctattcg catgcaggcg
     1741 gcgactacca ctttctgcgc cgcgcatacg ggcgctcggt ggccttcctg ttcggttggg
     1801 cccgcttctc ggtgatcacc accggctcca ttgccctgct cggcttcgta ttcggcgact
     1861 acatgaacca ggtctggccg atcggcggtt tcggcatcct gtccggcccg acgctgtatg
     1921 cggcggtctc gatcgtgatc ctgtcgttca tcaacatgcg cagcacgatg tccggcactt
     1981 atacccaaac ctggctgacc gctctcgagg tgggcggtct gctgatcatt gtcgccgccg
     2041 ccctctatct cgccggcacc ggcacgccgc cggcgacgct gcccgccgcc gatgctcccg
     2101 caggcgtgcc gagcgccgcc gcgtttgggc tggcaatggt attcgtgctg ctgaccttcg
     2161 gcggctggaa cgaggccgcc tacatcagcg ccgagttgcg cgacggccgg cgcggcatcg
     2221 tcaaggcgct gatctggtcg atcgtcatca tcaccgcggt ctacatgctg gtgatctggg
     2281 cgtactggcg cgcgctcggc atcgagggca tggccaactc gcaggcgatg gccgccgacg
     2341 tgctcggcgt cgcattcggg ccggtcggcg ccatgtcgat ctcgctgatg gtcgcggtgg
     2401 cggcgctgac atccatgaac gccaccatga tcgtcggcgc ccgtacgaac tatgcggtcg
     2461 gccaggactg gcattcgctg aagttgctca gccactggga cgacgaacgc gacgccccgc
     2521 tcaacgcgct gcgcgtgcag acgcttgccg cgctgctgct ggtgctggtg ggcgcgtgga
     2581 ccggcgcggg cttccgctcg atggtcgaat tcaccgcgcc ggtgttctgg ctgttcttcc
     2641 tgctctccgg tgtctcgctg ttcgtgctgc gctaccgcga gccaaatacc gagcgcccgt
     2701 tcaaggtgcc gctgtatccg ctgctgccgc tggtgttctg cgcgatgtgc gcgtacatgc
     2761 tgtggtccag cctgtcctat gtttacagcc aatcgctggg cggactgaac gccgcctgga
     2821 tcggcgtggg cgtgctggcc atcggtctcg tcctgttgct gctggtgcgt gcgcagcatg
     2881 cgcgccagca acgttctgcc gtgaaaacct tttgaggaga catcgatgaa cgcaggttct
     2941 gctttctccg cgcgcaccgt gcgcctgttg tgggccgcgg cgatgctggc ggctctcatg
     3001 ttgctgggca gcggaccggc ccacgcccag tccaccacgg agccggttcg tccacctgat
     3061 gtgccgtatg tgccgacgcc gcagccagtg gtcgacaaga tgctggaaat ggcgcgcgtc
     3121 ggcaaggacg acgtggtcta cgacctcggc tgcggcgacg gccgcatcgt gattaccgcc
     3181 gccagcgagt tcggcgcgcg cggcgtgggc atcgacatca acccggtgcg tatcgcggag
     3241 gcgaaggaca atgcacagaa ggccggcgtg accgacaagg tcgagttcaa ggtcggcgat
     3301 ctgttcgaat ccgatttctc caatgcgact gtcgtgacgc tgtatctgct gccggaaatc
     3361 aaccgcaagc tgcgtccgca actgtggcgc cagctgaagg tcggcacgcg cgtggtctcg
     3421 cacgatttcg acatgggcga cgaatggccg ccggaaaagg tcgaacaggt cggctacaag
     3481 acgatctact actggaccat caccgacgcc aacaagaagg cggtgaagta gtgatgaaca
     3541 gcgtgcggcc gccccgccgc acgcactatg cacccttggg gaccacgttt ccgggcggcc
     3601 tcggctcctg gatgaaacgc acaaggtcct ccatcggcag tggcgggctg aacagataac
     3661 cctggatctc gtcgcagccg ttctccctca ggaaggtcag ctgctcctgg ctcgacacgc
     3721 cttccgcaat cacatccagc cccaggctgt ggccgagcga gatcaccgcg cgcgcaatga
     3781 tcgcgtcgtt cggatcggcg ccgatgtcgc gcacgaagga ctggtcgatc ttcagcaggt
     3841 cgaccgggaa acgctgcagc tggctcaggc tcgagtaacc ggtgccgaag tcgtcgatcg
     3901 ccaggcacaa gcccatctcc ttcagttcac caagtacacg gatggcctcc tccggattgc
     3961 gcatcacgag gctttccgtc agttcgagtt cgagggaggc ggccgccagc ccgctcgcgc
     4021 tgagcgcacg ctgcacaagg gaaacgattg tcttcggctc gaactggcgt gccgaaatgt
     4081 tcaccgccat gcgcagtggc ggcagaccgc gcgcctggag cgccttgttg tgcgcgcagg
     4141 ccgtgtgcag cacccactcg ccgatggcgt tgatcaggcc ggtctcctcg gccagcggaa
     4201 tgaaggccga cggcgggatc gaaaccggcc cgcgcccatg gcgcggaaac cagcgcagca
     4261 aggcttccgc gccgaccacc tgggaggtgc gcaggtccag cttgggctgg tagtgcagct
     4321 cgaactcgtc gttcgccagc gcgcgccgca ggctcgcctc catcgacagt cgctcggtca
     4381 cccgacggct catcgccggc gtgaagaact ggaaattgtt gcgcccgcgc tccttggcca
     4441 ggtgcatcgc cgcatcggca ttcttcagca gcgtgtcggc gtcgtcgccg tcgcgcggat
     4501 acaggctcag gccgatgctg cagctgacaa agaactggtg ttcttccacg tagaacggct
     4561 ggtctatcgt tttgaggatc ttgtccacca gcatgccgac ctgcgtctcg tcgttatagc
     4621 cgacgaggat gaaggcaaat tcatctccgc catagcgcgc caccgtgtcc tccccctgca
     4681 ggcagctcaa caggcgggtg gcgaccgact tcagcaaggc atcgccgatg gtatggccga
     4741 ggctttcgtt gatgaacttg aagttgtcga gatcgatgaa gccgaccacg acggtagtgc
     4801 cgagccggtg cgcggtggcg attgccgcgc gcaggcggtc atgcaggacc ttgcggctgg
     4861 ccagcgtggt cagcgcatcg acgttgatca ggcgctccag ctcctgctcg tagcgcttga
     4921 ttgcagtcat gtcgttgatc acgccgacga aatgcgtgac ctcgccgtgc ggatcccgaa
     4981 ccggggcgat gtgaacgtcg ttccagaaca gcgttccgtc cttgcggtag ttgcgcagca
     5041 ggaccgcgca ttcctcgcgc cggtcgacgg ccgcgcgcag ggtcgccagt gcaggctggt
     5101 cgcggtctcc tgcctgcagg aagcggcagt tgcgcccgag agcttcattc atcgtgtagc
     5161 cggtaatgcg ctcgaaggcc ggattgacga aggtaatgtt ggacgcgagg tcggtgatga
     5221 tgatggcatt cacgctggcc tgaacggccc ggtcgcgcag cctcaacgcc tcttccgcac
     5281 ggcggcgtgc gctgatgtcg acgcccattc cgcatactcc ggcgcggccg tcgagctcga
     5341 tgggcgcgcc ggtgaagaaa tacggaatac gctcgcccga gcgggtcagg aaatcggctt
     5401 cgacggaatt gctcgctccg gccagcacgc cgtgcaaggc ctgcatcacc tgctcgcgtt
     5461 ggtccggatc aggaaaaaaa tcttcgacgc gcgcgtggac catctcatcg gcgctgtagc
     5521 ccgcgatcga ctcgtagttg cgattccagc gcaggaagcg ccccttgcaa tcgagcacgt
     5581 aaaccaggcc gggaaggctg tcgatgatcg cctgtgacag cagggcttcg cgcacagccc
     5641 tctgcgcgct cgcattgagc gcgcggtttg cctgctgcaa atcctcggtg cgtgcctgca
     5701 cctcgcgctc caactgcata tgcgagcgct ccagcgcctg ctgcgcctga tcccgttcgg
     5761 ccaggagcgc ccgcgtttcc tcgagcttgc tgcgcagctc aatctgcgca accacttcat
     5821 gcgccagcac acgcagggcc tgctcctgca gcggcgacaa gccccgcgcc acccggtcca
     5881 tcacgccgag actgccgatc gcgtgtccgt ccgcggtgac cagcggcgca ccggcataga
     5941 agcggatccc gggatcctgc cgcaccagcg gattgtcggc gaaacgcgga tcggtgtacg
     6001 cgtcctcgac gaccagcagt tcccgttgc
//
LOCUS       DATLGF010000014         4654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_16817,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000014 DATLGF010000000
VERSION     DATLGF010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16817"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /gene="prpF"
                     /locus_tag="VM406_00445"
     CDS             <1..1115
                     /gene="prpF"
                     /locus_tag="VM406_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010809523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HVL74455.1"
                     /translation="EDLPEGARLPGAVRDAFLMRVIGSPDPYGKQIDGMGGATSSTSK
                     TVIVAPSTKPDHDVDYLFGQVSIDRPFVDWSGNCGNLSAAIGPFAISNGMVDRARIPD
                     NGIATVRIWQANIGKTIIAHVPMTDGAVQETGDFELDGVTFPAAEIQLEFMDPAAEEE
                     GAGGSMFPTGNLVDDLEVPGVGTLKATMINAGIPTIFVNAEAIGYTGTELQEAINGDA
                     KALAMFETIRAHGALRMGLIKSPDEAAKRQHTPKIAFVAPPKDYVASSGKEVKAGDID
                     LVVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGERTAVRFGHPSGTLRVG
                     AEARRVDGEWVVTKAIMSRSARVLMEGWVRVPRDAF"
     gene            1192..3102
                     /locus_tag="VM406_00450"
     CDS             1192..3102
                     /locus_tag="VM406_00450"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HVL74456.1"
                     /translation="MSYADFYQRSITDPDAFWGEQARLIDWEKPYSQVLDYSRPPFAR
                     WFVGGTTNLCHNAVDRWVASQGDKPALIAISTETGSETVYTFSQLQAEVMRMAAIMQS
                     LGVSRGDRVLIYMPMVPEAAFAMLACARIGAVHSVVFGGFASNSLATRIDDAKPSLIV
                     SADAGSRGGKAVAYKPLLDEAIRLAHHKPGKVLLVDRGLVPMERVAGRDEDYARLREQ
                     HIDAQVPVTWLESNEVSYILYTSGTTGKPKGVQRDVGGYAVALAASMKYIFCGNPGET
                     YFSTSDIGWVVGHSYIVYGPLIAGMATVMYEGLPVLATPRPDAGIWWSIVEKYRVTRM
                     FSAPTAVRVLKKQPPEFMKKYDLSSLKALYLAGEPLDEPTSSWIAGELGVPIIDNYWQ
                     TETGWPILSVAHGVEHTPTRLGSPGVPMYGYKVKLINEATGALCGANEKGVVVIEGPL
                     PPGCMQTVYGDDERFVKTYWSSTRSNAGEQLYSTFDWGIRDADGYYFILGRTDDVINV
                     AGHRLGTREIEESISSHPNVSEVAVVGVEDKLKGQVAMAFAVLKHPDAVATPEGRKAQ
                     EAEIMAVVDKQLGAVARPARVHFVTLLPKTRSGKLLRRSIQAICEGRDPGDLTTIEDP
                     TSLQQVRAALQG"
     gene            complement(3119..4054)
                     /locus_tag="VM406_00455"
     CDS             complement(3119..4054)
                     /locus_tag="VM406_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HVL74457.1"
                     /translation="MIDRHRAWTLFKRVLTVAFISIVLVLLVTQLRDVDWGEVRASLG
                     RYQAPVLLLAFALAAASHALYGLFDQIGRVYTGHRLARLRVARIAFVSYAFNLNMGAM
                     IGGVGFRYRLYSRYGLDGATITRVMGLSVAANWLGYLVVGGTVFAMREIALPAGWEIG
                     TAGLQWIGFAMLAAAAGYLAACGLSRRRSWTVRGHEIVLPSLRMALLQVLISSLNWML
                     IAALIHVLLFGQVSYLTVLAVFLISAIAGVLAHIPAGLGVIELVFVTLLGHLLAPSEL
                     IAALFAYRAVYYLAPLALALFVYLHLETRAKAHAQ"
     gene            complement(4047..>4654)
                     /gene="clsB"
                     /locus_tag="VM406_00460"
     CDS             complement(4047..>4654)
                     /gene="clsB"
                     /locus_tag="VM406_00460"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HVL74458.1"
                     /translation="TNDIERHYRHAIQAARREVWIACAYFFPSYSLLREMRQAALRGV
                     RVRLILQGKPDMVIVPIAARLLYGCLVRDGVSIYEYCRRPLHAKVALVDDEWATVGSS
                     NLEPFSLSLQLEANVMLRAPEFNRILRASLTQMIEQHCVEVEPQSLPRDSWWRSAVGV
                     AVFHFLRHFPWWAERLPTHRPRLTVLGAEERKQFTEGGGND"
BASE COUNT          857 a         1514 c         1568 g          715 t
ORIGIN      
        1 atgaagacct gcccgaaggc gcgcgcctgc ccggcgcggt gcgcgatgcc ttcctgatgc
       61 gcgtgatcgg cagccccgac ccctacggca agcagatcga cggcatgggc ggtgcgacct
      121 ccagcaccag caagaccgtg atcgtcgcac ccagcacgaa gccggatcac gacgtcgact
      181 acctgttcgg ccaggtctcg atcgacaggc ccttcgtcga ctggagcggc aactgcggca
      241 atttgtcggc ggcgatcggc ccgtttgcga tcagcaacgg catggtcgat cgggcacgga
      301 ttccggacaa cggcatcgca acggtgcgca tctggcaggc caacatcggc aagaccatca
      361 tcgcgcatgt gccgatgacc gatggggcgg tgcaggagac cggcgatttc gaactcgacg
      421 gtgtgacctt cccggccgcc gaaatccagc tcgagttcat ggatccggcc gccgaggagg
      481 agggcgcggg gggatcgatg ttcccgaccg ggaacctcgt cgacgatctc gaagtgcccg
      541 gcgtaggtac cttgaaggcg acgatgatca acgccggcat tcctacgatt ttcgtcaacg
      601 ccgaggcgat cggctatacc ggcaccgaat tgcaggaagc gatcaacggc gacgcgaagg
      661 cgctggcgat gttcgaaacc atccgcgcgc acggcgcgct gcgcatgggc ctgatcaagt
      721 cgccggatga agcggccaag cgccagcaca cgccgaagat cgcgttcgtg gcgccgccga
      781 aggattacgt cgcgtccagc ggcaaggaag tcaaggccgg cgacatcgat ctggtcgtgc
      841 gcgcgctgtc gatgggcaag ctgcaccacg cgatgatggg cacggccgcg gttgcgatcg
      901 gcactgctgc cgcgattccc ggcacgctgg tcaacctcgc cgccggcggc ggggagcgca
      961 ccgcggtgcg cttcggtcat ccgtccggca ccttgcgcgt gggggccgag gcgcggcgcg
     1021 tcgacggcga atgggtggtc accaaggcca tcatgagccg cagcgcgcgc gtgctgatgg
     1081 agggctgggt gcgggtgccc cgcgacgcct tttgaacccc ccaatctgac gtaagtccgc
     1141 cgtaagccgc tttaccgata ctggacggaa cagacgcaag gaggagaccc gatgagctat
     1201 gccgatttct accagcgctc gatcaccgat ccggacgcct tctggggcga gcaggcgagg
     1261 ctgatcgact gggaaaagcc gtattcgcaa gtgctcgatt acagccggcc gccgtttgcg
     1321 cgctggttcg tcggcggcac caccaacctg tgccacaacg cggtcgaccg ctgggtggca
     1381 agccagggcg acaagccggc gctgatcgcg atctcgaccg agaccggcag cgagacggtc
     1441 tacacgttct cgcagctgca ggcagaagtg atgcgcatgg cggcgatcat gcagtcgctc
     1501 ggcgtgtcca ggggcgaccg cgtgctgatt tacatgccga tggtgcccga ggcggcgttt
     1561 gcgatgctgg cctgcgcgcg catcggggcc gtgcactcgg tggtattcgg cggcttcgcg
     1621 tccaacagcc tcgccacccg catcgacgat gccaagccca gcctcatcgt gtctgccgat
     1681 gccggctcgc gcggcggcaa ggcggtcgcg tacaagccac tcttggacga ggcgatccgg
     1741 ctcgcgcatc acaagccggg caaggtgctg ctggtggatc gcggactggt tccgatggaa
     1801 cgcgtcgcag ggcgcgacga ggactacgcg cgcctgcgcg agcagcatat cgatgcacag
     1861 gtgccggtga cctggctcga atcgaacgag gtctcgtaca tcctctacac ctccggcacg
     1921 accggaaagc ccaagggcgt gcagcgcgac gtcggcggct atgcggtcgc gctggccgca
     1981 tcgatgaaat acatcttctg cggcaacccg ggggagacgt atttttccac ctcggacatc
     2041 ggctgggtcg tcggccattc gtacatcgtc tacgggcccc tgatcgccgg catggcgacg
     2101 gtgatgtacg aaggcctgcc cgtgctggcc accccgcggc ccgacgccgg catctggtgg
     2161 agcatcgtcg agaagtacag ggtcacccgc atgttctcgg cgccgaccgc ggtgcgcgtg
     2221 ctgaagaagc agccgcccga gttcatgaag aagtacgacc tgtcgtcgct caaggcgctg
     2281 tacctggccg gcgaaccgct ggacgagccg acctcctcct ggatcgccgg cgaactcggc
     2341 gtgcccatca tcgacaacta ctggcagacc gaaaccggct ggccgattct ctcggtcgcg
     2401 cacggcgtcg agcacacgcc gacgcgcctg ggcagccccg gcgtgccgat gtacggctac
     2461 aaggtcaagc tgatcaacga ggcgaccggc gccttgtgcg gcgcgaacga gaaaggcgtg
     2521 gtcgtgatcg aagggccgtt gccgccgggc tgcatgcaga ccgtgtacgg cgatgacgaa
     2581 cgtttcgtga agacctactg gtcctctacg cggtcaaacg ccggcgagca gctgtactcg
     2641 accttcgact ggggcatccg cgatgcggac ggctattact tcatcctcgg ccgcaccgac
     2701 gacgtgatca acgttgccgg ccaccggctc ggcacgcgag agatcgagga gagcatctcc
     2761 agccatccga acgtgtcgga ggtggccgtg gttggcgtcg aggacaagct gaaggggcag
     2821 gtggcgatgg cgttcgcggt cctgaagcat cccgatgcag tcgccacgcc cgaggggcgc
     2881 aaggcgcagg aggcggagat catggccgtg gtggacaagc agctgggcgc ggtcgcgcgt
     2941 ccggcgcgcg tgcatttcgt cacgctgctg ccgaaaacgc gttccggcaa gctgctgcgc
     3001 cgttcgatcc aggcgatctg cgaagggcgc gatcccggcg acctgacgac catcgaggac
     3061 ccgacctcgc tgcaacaggt gcgcgccgcg ttgcagggct gagcctgggt cttcatcctc
     3121 actgggcgtg ggccttggcc cgcgtttcca ggtggagata cacgaacagt gcgagcgcca
     3181 gcggcgccag gtagtagacc gcgcgatagg cgaacagcgc ggcgatcagc tcgctcggcg
     3241 cgaggaggtg gccgagcagg gtgacgaaca ccagttcgat caccccgagc ccggccggga
     3301 tgtgggcgag cacgccggcg atcgcgctga tcaggaacac cgccagcaca gtcaggtacg
     3361 agacctgccc gaacagcagt acatggatca gcgccgcaat cagcatccag ttcagcgagg
     3421 aaatcaggac ctgaagcaag gccatgcgca gcgagggcag cacgatctcg tgcccgcgca
     3481 cggtccagct gcgccgcctc gacaagccgc aggcggcaag atagccggcg gccgcggcca
     3541 gcattgcaaa gcctatccat tgcagtccgg cggtgccgat ctcccagccg gcgggcaagg
     3601 cgatctctct catcgcgaat accgtcccgc ccaccacgag gtagccaagc cagttcgccg
     3661 ccacgctcag tcccatcacc cgggtgatcg tggcgccgtc cagcccgtag cgtgaataga
     3721 gtcggtagcg aaatcccacg ccgccgatca tcgcgcccat gttcaggttg aacgcgtagc
     3781 tcacgaaagc gatccgcgca acccgcaggc gagcgaggcg gtgaccggta tagacgcgtc
     3841 cgatctggtc aaacaggccg taaagcgcgt ggctggcggc cgccagcgcg aatgcgagca
     3901 gcagtaccgg tgcctgatag cggccgagcg acgcgcgtac ctcgccccaa tcgacgtcgc
     3961 gcaactgggt caccagcagt accagcacga tgctgatgaa ggccaccgtc aatacccgct
     4021 tgaacagcgt ccaggcgcga tggcggtcaa tcattcccgc cgccctcggt gaattgcttg
     4081 cgttcctcgg ctccaagcac ggtcaggcgc gggcggtgcg tcggcaggcg ctcggcccac
     4141 cagggaaagt ggcgcaggaa gtggaacaca gcaacgccca ccgcgctgcg ccaccagctg
     4201 tcgcgcggca ggctttgcgg ttccacttcc acgcagtgct gctcgatcat ctgcgtcagc
     4261 gacgcgcgca ggatccggtt gaattcgggg gcacgcagca tcacgttggc ctcgagctgc
     4321 agcgacagac tgaacggttc gaggttgctg gagccgacgg tggcccattc gtcgtcgacg
     4381 agcgccacct tcgcatgcag cgggcgccgg caatattcgt agatggaaac gccgtcccgt
     4441 accaggcatc cgtacagcag gcgcgccgcg atggggacga tgaccatgtc cggcttgccc
     4501 tgcaggatca ggcgcacgcg cacgccgcgc agcgctgcct ggcgcatctc gcgcagcagc
     4561 gagtagctcg ggaagaagta ggcgcaggcg atccagactt cccggcgcgc ggcctggatc
     4621 gcatgtcgat agtgccgctc gatgtcattc gtat
//
LOCUS       DATLGF010000015         4986 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_16826,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000015 DATLGF010000000
VERSION     DATLGF010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16826"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="VM406_00465"
     CDS             complement(<1..1295)
                     /locus_tag="VM406_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HVL74459.1"
                     /translation="MHSDKRAPLAGFSRKTLSAAVVSALLVSNAFAAGLGRMTVLSSL
                     GQPLRAEIELTAVSKDEIGALAAKLASSEAFRQANIDFNAALLSLRFNVENRGDRHFI
                     RVTSSQPLNEPFVDMLLELSGPNGRLIREYTFLLDPPDLRMTQSASVAPAPVPQLGSA
                     GTQQAAAVSQQPGTAAPRAAAVPSASRAASPAAGAEAGVTDYQVRQGDTLTQIAGRVK
                     PAHVSLDQMVVALYQSNQDAFDEKNMNRLKAGQILNVPDAETAAAVEPAAARSIIIAQ
                     AADFNAYRQRLAGQVASASARRAGEARQAAGGQITARVEERAGPASASPDRLQLSHAT
                     ADGAGNVTAEDLIARDKALAEAQARVKELERNVSDLQKLLELKNASLAEQQNQAEAAR
                     SGATPEPAAPVAAAAPAAAPEPVQADAAPTPGSAAPAPVA"
     gene            complement(1666..2772)
                     /gene="asd"
                     /locus_tag="VM406_00470"
     CDS             complement(1666..2772)
                     /gene="asd"
                     /locus_tag="VM406_00470"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HVL74460.1"
                     /translation="MKSVGLVGWRGMVGSVLMQRMQEESDFDHIEPVFFSTSNAGGRA
                     PAMAKNETALKDANNIDELKKCDIIITCQGGDYTTEIFPKLRAAGWNGYWIDAASTLR
                     MADDAVIVLDPVNLHVIKDALGRGVKNYIGGNCTNSILLMGVNGLFKEGLVEWVSSMT
                     YQAASGGGANHMRELLKGMGVIHAAVADELATPSSAILDIDRKVAHTIRNDVPTEFFP
                     APLAGGLIPWIDKQLDNGQSKEEWKGQAEVNKILGTNHVIPVDGLCVRIGAMRCHSLA
                     LTLKLKKDVPLAQIEEIIRAGNPWVKWVPNERSATERELTPASITGNLQVGVGRVRKL
                     NMGPEYLSAFVIGDQLLWGAAEPLRRMLRILLEG"
     gene            complement(2800..3870)
                     /gene="leuB"
                     /locus_tag="VM406_00475"
     CDS             complement(2800..3870)
                     /gene="leuB"
                     /locus_tag="VM406_00475"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HVL74461.1"
                     /translation="MKIAILPGDGIGPEIIEQATRVLHALDEKFELEAAPVGGAGYEA
                     HGHPLPDATLKLAQEADAVLFGAVGDWKYDNLERALRPEQAILGLRKNLGLFANLRPA
                     ILYPELAGASTLKPEVVSGLDILIVRELTGDIYFGQPRGVRECPDGPFKGAREGFDTM
                     RYAEPEIRRIAHVAFRAAQKRGKRLTSVDKANVLETFQFWKDIVTDVHKEYPDVALDH
                     MYVDNAAMQLVRAPKKFDVIVTGNMFGDILSDAAAMLTGSIGMLPSASLDANNKGLYE
                     PSHGSAPDIAGKGVANPLATILSAAMMLRFSLNKAEQADRVEKAVKKVLAQGLRTPDI
                     YEEGTTKVGTKEMGDAVVRALG"
     gene            complement(3965..4612)
                     /gene="leuD"
                     /locus_tag="VM406_00480"
     CDS             complement(3965..4612)
                     /gene="leuD"
                     /locus_tag="VM406_00480"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HVL74462.1"
                     /translation="MDKFTVHEGLVAPLDRANVDTDAIIPKQFLKSIKRSGFGPNLFD
                     EWRYLDHGEPGMDNSTRPLNPDFVLNQPRYQGASILLTRKNFGCGSSREHAPWALQQY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTESQVDQLFNEVKAFPGYKLVIDLDKQTVA
                     TSNGGTVYPFEIDAFRKYCLLNGFDDIGLTLRHADKIRAFEERHLAAHPWLANTI"
     gene            complement(4661..4783)
                     /locus_tag="VM406_00485"
     CDS             complement(4661..4783)
                     /locus_tag="VM406_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HVL74463.1"
                     /translation="MKKAFMLLGVLAILAGCNTFQGVGKDVKRAGEAIENAARR"
     gene            complement(4805..>4986)
                     /locus_tag="VM406_00490"
     CDS             complement(4805..>4986)
                     /locus_tag="VM406_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HVL74464.1"
                     /translation="SMCLAMNADRLEPGERCASTSNRNFEGRQGQGGRTHLVSPAMAA
                     AAGIAGHFVDVRAMR"
BASE COUNT          850 a         1607 c         1560 g          969 t
ORIGIN      
        1 gcgacggggg ccggcgctgc gctgccaggc gtcggcgcgg catcggcctg taccggctcc
       61 ggcgctgctg cgggcgctgc tgcggcaacc ggcgcggcgg gctccggcgt cgcccccgac
      121 ctcgccgcct cggcctggtt ctgctgttcg gccaggctgg cattcttgag ctcgagcagc
      181 ttctgcaggt cgctgacgtt cctctcgagt tccttcacgc gcgcctgcgc ctcggcgagc
      241 gccttgtcgc gcgcgatcaa gtcctcggcc gtcacgttcc ctgcgccgtc tgccgttgca
      301 tgcgaaagtt gcaggcgatc cggcgacgcc gacgccgggc ctgcgcgctc ttccaccctc
      361 gcggtgatct gcccgcctgc ggcctgacgt gcttcgccgg cacgacgggc gctcgcggac
      421 gccacctggc cagccaggcg ctggcggtat gcgttgaagt cggccgcctg ggcaatgatg
      481 atgctgcgcg ccgccgcggg ctcgaccgct gccgcagtct ccgcgtcggg cacgttgaga
      541 atctggccgg ccttcaaccg gttcatgttc ttctcgtcga aggcatcctg gttgctctgg
      601 tacagcgcga ccaccatctg atcgagcgaa acgtgagccg gtttgacgcg acccgcgatc
      661 tgggtaagcg tatcaccctg ccgcacctgg taatcggtta ccccggcctc ggcacctgcc
      721 gccggggatg ccgcgcgtga agcagacgga accgcagcgg cgcgaggcgc cgccgtgccg
      781 ggctgttggg acactgccgc tgcctgctgc gtgccggccg aaccgagttg cggcacaggc
      841 gccggtgcca ctgacgcaga ctgggtcatg cgcaggtctg gcggatcgag caggaacgtg
      901 tattcccgga tcaggcggcc gttcggtccg ctcagctcga gcagcatgtc gacgaaaggc
      961 tcgttcagcg gctggctcga cgtcacccgg atgaaatggc ggtcgccgcg gttttcgaca
     1021 ttgaaacgca gcgaaagcaa ggccgcgttg aagtcgatgt tcgcctgacg aaatgcttcg
     1081 gacgacgcga gtttggcagc gagcgcgccg atctcgtcct tcgacacggc ggtcagctcg
     1141 atctcggcgc gcagcggctg gccgagcgag gacaggactg tcatgcgccc caggccggcg
     1201 gcaaaggcgt tcgagacgag cagtgccgag acgacggccg cactgagcgt cttcctgctg
     1261 aaaccggcga gcggcgcgcg cttatctgag tgcatcttgg agggcataaa agacagtccg
     1321 tgtcggaaac gaggcgccgc aacggcgcct cgaaaaatta ttcagggcaa ctttcaccat
     1381 atcatcatgg acttacggcc gcaagtgtat ccaagttgcc aactcatgca atagcgccaa
     1441 cgacctcatg cggggataaa aaccggtgca atcgcgggcg ttccatgtgc gatcgcgtgt
     1501 attgccgcat gacgcgaagc agatgctgca gattgcaggg cgcgatcaaa agttccgcag
     1561 cgtaagaacg ggccgtgggc cgcaagattt tgttgtccgc acacaacgca attggggccg
     1621 gcccgccgca aggcgagccg gccccaacct tggccggaaa gcgaatcagc cttccagcag
     1681 gatgcgcagc atccggcgca acggttccgc ggcgccccag agcagctggt cgccgatcac
     1741 gaaggccgac aggtattcgg gccccatgtt cagcttacgc acgcgtccga cgccaacctg
     1801 caggttgccc gtgatcgaag cgggtgtcag ctcgcgctcg gtggccgaac gctcgttcgg
     1861 cacccatttc acccacggat tgccggcgcg gatgatctct tcgatctgag cgagcggaac
     1921 atccttcttc agcttcagcg tcagtgccag gctgtggcag cgcatcgcgc cgatgcgcac
     1981 gcacaggccg tccaccggga tgacgtggtt ggtgccgagg atcttgttga cttcggcctg
     2041 ccccttccat tcttccttgg actggccgtt gtcgagctgc ttgtcgatcc acgggatcag
     2101 gccgccggcc aaaggcgccg ggaagaattc agtcggcacg tcgttgcgga tcgtatgggc
     2161 aaccttccga tcgatgtcca agatcgccga ggacggcgtg gccagctcgt cggccactgc
     2221 ggcgtgaatc acgcccatgc ccttgagcag ttcgcgcatg tgattcgcgc cgccgccgga
     2281 agcggcttgg taggtcatcg agctgaccca ttcgaccagg ccttccttga acaggccgtt
     2341 gacgcccatc agcaggatcg aattcgtgca gttgccgccg atgtaattct ttacaccccg
     2401 cccgagcgca tccttgatca catgcagatt gaccggatcg agcacgatga cggcgtcatc
     2461 ggccatgcgc agcgtcgagg ctgcgtcgat ccagtagccg ttccagcccg cagcgcgcag
     2521 tttcgggaaa atctcggtcg tgtagtcgcc gccctggcag gtaatgatga tgtcgcattt
     2581 tttcagctca tcaatattgt tggcgtcctt gagcgcggtt tcattcttcg ccatcgcggg
     2641 agctctgccg cccgcattgg atgtcgaaaa gaacaccggt tcgatatgat cgaaatcgct
     2701 ctcttcctgc atgcgctgca tcaggaccga gcccaccata cctcgccaac ctaccagacc
     2761 tactgacttc acgattttct ccctcgtcaa ttaaaccatc tatccaagcg cccgtaccac
     2821 agcatcaccc atctccttcg tgcctacctt ggtcgtacct tcttcgtaaa tatccggtgt
     2881 gcgcaatcct tgcgcaagca ctttcttgac cgccttctcg acgcggtcgg cctgctctgc
     2941 cttgttgagc gaaaaacgca acatcatcgc cgccgacagg atggttgcca gcgggttggc
     3001 gacgcctttt ccggcgatgt caggcgcgga gccgtgcgat ggttcgtaca accccttgtt
     3061 gttcgcgtcc agcgacgcgg aaggcagcat gccgatcgag ccggtcagca tggccgccgc
     3121 gtctgacagg atgtcgccga acatgttccc ggtcacgatc acgtcgaact tcttcggcgc
     3181 gcgcaccagc tgcatcgcgg cgttgtcaac gtacatgtgg tcgagcgcga catccgggta
     3241 ttccttgtgc acatcggtca cgatgtcttt ccagaactgg aaggtttcga gcacgttcgc
     3301 cttgtccaca ctggtcaggc gctttccgcg cttctgcgcg gcccggaagg cgacgtgggc
     3361 gatgcggcgg atttccggct cggcatagcg catggtatcg aagccttcgc gcgcgccctt
     3421 gaacgggccg tccgggcatt cgcgcacgcc gcgcggctgg ccgaaataga tgtcgccggt
     3481 cagctcgcgc acgatcagga tatcgaggcc ggacacgacc tcgggcttga gcgtcgaggc
     3541 gccggccagc tccgggtaga ggatcgccgg gcgcaggttg gcaaacaggc caagattctt
     3601 gcgcaggcca aggatcgcct gctcagggcg cagcgcgcgt tccaggttgt cgtatttcca
     3661 gtcgccgacc gcgccgaaca acaccgcatc ggcctcctgg gccagcttca gcgtggcgtc
     3721 cggaagcgga tggccatggg cctcgtagcc ggcgccgccg accggtgcag cttccagttc
     3781 aaacttttcg tccagcgcgt gcaacacgcg cgtcgcctgt tcgatgattt cgggaccgat
     3841 gccgtcgccg ggcaggattg cgattttcat tgtcgtattc ggtgcggtgg ttctcatgac
     3901 gctccgtcaa tgccgaaacc ggcgctcaag cgggcgcccg gctccggcat gcgccggaac
     3961 gacttcaaat ggtgttggcg agccaggggt gagcggccag atggcgctct tcgaacgcgc
     4021 ggatcttgtc cgcgtgccgc agcgtcagcc cgatgtcgtc gaagccgttc agcaggcagt
     4081 acttgcggaa agcgtcgatt tcgaacgggt agaccgtgcc gccgttgctc gtggcaaccg
     4141 tctgcttgtc caggtcgatc accagcttgt agcccgggaa ggccttgacc tcgttgaaca
     4201 actggtcgac ctgcgactcc gtgagcacga tcggcagcaa gccgttcttg tagcagttat
     4261 tgaaaaagat atccgcaaag ctcggcgcga tcaccgcgcg aaagccgtat tgctgaagcg
     4321 cccagggcgc gtgctcgcgc gaggagccgc agccgaagtt cttgcgcgtg agcaggatcg
     4381 acgcaccctg ataacgcggc tggttcagca cgaagtccgg gttgagcggg cgcgtcgaat
     4441 tgtccatgcc aggctcgccg tggtcgagat agcgccactc gtcgaacagg ttgggaccga
     4501 agccggagcg cttgatcgat ttcaggaatt gcttggggat gatggcgtcg gtatcgacat
     4561 tcgcgcggtc cagtggcgca accaatcctt catgtacggt gaatttgtcc atattcgctt
     4621 cgttcgtcgg cgacgaacct tctctcatcg gttggcggcg ttatcttctg gcggcgttct
     4681 cgatcgcttc gccggcccgc ttcacatcct tgcctacgcc ctggaaggtg ttgcaacccg
     4741 ccagaatcgc aagaactcca agcagcatga acgctttctt catattttcc tccctgagaa
     4801 acgactagcg catcgcccgg acgtcgacga aatgcccggc gatgccggcg gccgcggcca
     4861 tcgccggcga caccagatgc gtgcgtccgc cctgcccctg ccgcccttcg aagttgcggt
     4921 tcgaggtcga ggcgcagcgc tcgcccggct ccagccggtc ggcgttcatc gccaggcaca
     4981 tcgagc
//
LOCUS       DATLGF010000016         2371 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_174769, whole genome shotgun sequence.
ACCESSION   DATLGF010000016 DATLGF010000000
VERSION     DATLGF010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174769"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VM406_00495"
     CDS             <1..466
                     /locus_tag="VM406_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HVL74465.1"
                     /translation="TDVVRWLADVCADRRIPFPAFYLADTMGWANPKEIQRRIGAVRE
                     LVPQARIGLHLHDTRGLGGANVFAALQMGVELFDSSVAGLGGCPFAGHKDGSASGNVC
                     TEDMVFMCQEMGIDTGIDLEKLVEASRLAERVIGRPLSGHLMHSGTLAAMRA"
     gene            613..1104
                     /locus_tag="VM406_00500"
     CDS             613..1104
                     /locus_tag="VM406_00500"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HVL74466.1"
                     /translation="MPESADADDLLMRKWQQITVISPYSRELGLQLHAVQPDWCVIRA
                     PYDPRTVGNPETGVVHGGVITALLDSCYGLAIFVRMARLRRMATLDLRIDYLKPATPG
                     RDILGGAVCYKLGHELAFVRGAAYHDSPDDPIATSSAIFMFTEGPAISLDEAVQALEA
                     PMP"
     gene            1101..1559
                     /locus_tag="VM406_00505"
     CDS             1101..1559
                     /locus_tag="VM406_00505"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HVL74467.1"
                     /translation="MNLTELIRSARASRNWDPLIDLVPYARFLNLRLDIKGDQFTACM
                     PFDRKLIGNPALPALHGGATGGFIECAGLFYLLWHMESADLPKTINFNFEYLRSGRPE
                     DTYANVFMVKQGQRVAHLRVEAWQSSPDKPIATGHGNFMLKAAPKTEGNS"
     gene            1556..>2371
                     /locus_tag="VM406_00510"
     CDS             1556..>2371
                     /locus_tag="VM406_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL74468.1"
                     /translation="MTQNAYRSIYRAGLFAGQTVIVTGGGSGIGRCIAHELASLGADL
                     ALVGRKIEKLQAVKDEIADAAAGGRVTIHSCDIRDEAGVRQMVADVLGTHGRIAGLVN
                     NAGGQYPQPVRDITLKGWDAVVRNNLHGGFLVSREAYTQWMEAHGGAIVNIIADMWMG
                     MPGMAHSGAARAGMLSFTETAACEWGHAGVRVNAVAPGFVASSGFDTYGPEMKAKFRS
                     LMKTSPVQRYGTEAEVSSAVVYLLSEGAAYITGSFVRVDGGVPNARQTWKLEEH"
BASE COUNT          418 a          781 c          796 g          376 t
ORIGIN      
        1 caccgatgtc gtgcgctggc ttgccgacgt gtgcgccgac cggcgcatcc cgtttccggc
       61 cttctacctg gcggacacga tgggctgggc caatccgaaa gagatccagc gccgcatcgg
      121 cgcggtgcgg gagctcgtcc ctcaagcgcg catcggcctg catctgcacg atacgcgcgg
      181 cctgggcggc gccaacgtct tcgcagcgct gcagatgggc gtggagctgt tcgacagctc
      241 cgtcgccggc ctgggcggct gccccttcgc cgggcacaag gacggctccg cctcgggtaa
      301 cgtttgtacc gaggacatgg ttttcatgtg ccaggagatg gggatcgaca ctggaatcga
      361 cctggaaaag ctggtcgagg cgtcgcgcct ggcggagcgc gtcatcgggc gtcccctgag
      421 tgggcacttg atgcatagcg gtaccctagc cgccatgcgg gcctgaatac ttccggcgtg
      481 tcgaagtttc cgctgtgctg ccggcgactc cgcgatctca ggaaccgtcc atgacgaagc
      541 atgccccggg agatagcctg gcgctgcgcc cgcgtcgggc ccctgcggac gccacattca
      601 cctggagaag acatgccgga atcggctgat gcagacgacc tgctgatgcg aaagtggcag
      661 cagatcaccg tgatctcgcc gtacagccgc gagctgggac tgcagctgca cgcggttcaa
      721 cccgactggt gcgtgatccg ggcgccatac gatccacgga cggtcggcaa ccccgaaacg
      781 ggcgtggtgc acggcggcgt gatcaccgcc ttgctcgaca gctgctacgg cctcgcgatc
      841 ttcgtgcgca tggccaggct caggcgcatg gccacgctgg acctgcgcat cgattacctc
      901 aagccggcca ccccggggcg cgacatcctg ggcggtgcgg tctgctacaa gctcggccac
      961 gaactggcgt tcgtgcgcgg cgccgcctac cacgacagcc cggacgatcc gattgcgacc
     1021 tcgtccgcga tcttcatgtt caccgaaggg ccggcgattt cgctggacga agcggtgcag
     1081 gcactggagg caccgatgcc atgaacctga ccgaactgat tcggtccgcg cgggcgagcc
     1141 gcaactggga cccgctgatc gacctggttc cgtatgcgcg gttcctcaac ctgcgactgg
     1201 acatcaaggg cgaccagttc accgcctgca tgcctttcga ccgcaagctg atcggcaacc
     1261 cggcgctgcc ggcgctgcac ggtggcgcga ccggcggatt catcgagtgc gccgggctgt
     1321 tctacctgct gtggcacatg gaaagcgccg acctgcccaa gacgatcaat ttcaacttcg
     1381 agtacctgcg ctcgggccgg cccgaagaca cctacgccaa cgtgttcatg gtcaagcagg
     1441 gccagcgcgt ggcccacctg cgcgtcgagg cctggcaatc gagccccgac aagccgatcg
     1501 ccaccggaca cggcaacttc atgctgaagg cggcgccgaa gacggaagga aattcatgac
     1561 acagaacgcc tatcgatcca tctaccgcgc cggcttgttc gcggggcaga cggtcatcgt
     1621 cactggcggc ggcagcggca tcggccgatg catcgcccat gaactggcca gcctgggcgc
     1681 agacctggcc ctggtcgggc gcaagatcga gaagctccag gccgtgaaag acgagatcgc
     1741 ggatgcggca gccggaggca gggtcaccat ccattcgtgc gacatccggg acgaagcggg
     1801 ggtcaggcag atggtggccg acgtgctcgg gacgcacggc cgcatcgccg gccttgtcaa
     1861 caacgccggc ggccagtatc cgcagcccgt gcgcgacatc acgctcaagg gctgggacgc
     1921 agtggtgcgc aacaacctgc acggcggctt cctcgtctct cgagaggcct acacgcagtg
     1981 gatggaagcg cacggaggcg ccatcgtcaa catcatcgcg gacatgtgga tggggatgcc
     2041 ggggatggcg cacagcggcg ccgcgcgggc cggcatgctg tcgttcaccg agacggcggc
     2101 ctgcgagtgg ggacacgcgg gcgtgcgtgt caatgccgtc gcgcccggct tcgtcgccag
     2161 cagcggcttc gacacctatg gcccggagat gaaggccaag ttccgcagcc tgatgaaaac
     2221 gtccccggtg cagcgctacg gaaccgaggc cgaagtatcc tcggcggtgg tctacctgct
     2281 ttccgagggg gcagcgtaca tcacgggctc cttcgtccgt gtcgacggcg gcgtgcccaa
     2341 tgcgcgacag acctggaagc tggaggagca c
//
LOCUS       DATLGF010000017         6289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_48557,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000017 DATLGF010000000
VERSION     DATLGF010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48557"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1492)
                     /locus_tag="VM406_00515"
                     /pseudo
     CDS             complement(1..1492)
                     /locus_tag="VM406_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450995.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
     gene            complement(1602..1973)
                     /gene="crcB"
                     /locus_tag="VM406_00520"
     CDS             complement(1602..1973)
                     /gene="crcB"
                     /locus_tag="VM406_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HVL74469.1"
                     /translation="MNWLAIGVGAALGAWLRWALALWLNSVHSTLPHGTLAANLAGGF
                     MVGIAVAFFSVHEGLPIAWRLFVVTGFLGGLTTFSTFSAESLLLLQRGDVLWAFAHSA
                     LHLFGSLAMCAAGFALYRALA"
     gene            complement(1970..2449)
                     /gene="moaE"
                     /locus_tag="VM406_00525"
     CDS             complement(1970..2449)
                     /gene="moaE"
                     /locus_tag="VM406_00525"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HVL74470.1"
                     /translation="MPVRVQIEDFDLSAEVAALRAGNAKVGAVAAFIGTVRDMNDGEG
                     VASMELEHYPGMTETALEEIVTEAKRRWPVFDATVIHRVGPLQPLDQIVLVAVTSAHR
                     GEAFAACEFIMDYLKTAAPFWKKEQTAQGARWVDARVSDDAALARWGLRSTNLAGDE"
     gene            complement(2451..2708)
                     /gene="moaD"
                     /locus_tag="VM406_00530"
     CDS             complement(2451..2708)
                     /gene="moaD"
                     /locus_tag="VM406_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010115001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HVL74471.1"
                     /translation="MRIQLRFFASVREALGTGAEMLELPADVRTVGDVRAFLRQRGGA
                     WEETLAEGRALRMAWNHQMTGADTPIADGGEVAFFPPVTGG"
     gene            complement(2710..3963)
                     /gene="glp"
                     /locus_tag="VM406_00535"
     CDS             complement(2710..3963)
                     /gene="glp"
                     /locus_tag="VM406_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HVL74472.1"
                     /translation="MQTSGKKPMLSAAEALDLLLAAARRITDTETLPTFEANGRVLAA
                     DQTSRLDVPPMDNTQMDGYAVRAADCAGGAATLPVAQRIPAGHVGQPLLPGTAARIFT
                     GAMIPEGADAVVMQEQCEVVGNALGNTAGDAVIVRHAPQPGEWIRRRGEDIRAGSTIL
                     AAGTRLRAQELGLAASVGLAHLPLVRRVRVAMFFTGDELTMPGERLKPGAIYNSNRFV
                     LRGLLENLGCEITDFGIVPDSLEATRETLCHAAERHDLVLTSGGVSVGEADFVKPAVE
                     AEGRLDMWQIAIKPGKPLAFGEVRRKFGSAFFIGLPGNPVSSFVTFLLFVRPFLLRMQ
                     GVQRVAPRAYAMRADFDWPKADRRNEFLRARLNGEGGLELYPNQGSAVLTSTVWGDGL
                     VDNPPGTTISRGDIVRFLPFSDLLD"
     gene            complement(3979..5439)
                     /gene="thrC"
                     /locus_tag="VM406_00540"
     CDS             complement(3979..5439)
                     /gene="thrC"
                     /locus_tag="VM406_00540"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HVL74473.1"
                     /translation="MQYLSTRGQAPAQSFSQILLGGLAPDGGLYLPIEYPKVSGAELD
                     AWRKLSYADLAFEVLKKFATDIPEQDLKALAHKTYTPQVYRNARPDEDARQITPLRVL
                     EERDGGKLVLQALSNGPTLAFKDMAMQLLGNLFEYALAKQDAELNIFGATSGDTGSAA
                     EYAMRGKKGIRVFMLSPHRKMSAFQTAQMFSLQDPNIINIAVEGVFDDCQDMVKAVSN
                     DLAFKSRYKIGTVNSINWARVVAQVVYYFRGYLAATGSNDQKVSFTVPSGNFGNICAG
                     HIARMMGLPIDKLVVATNENDVLDEFFRTGVYRVRKSAETYHTTSPSMDISKASNFER
                     FIYDLLGRDAARVNALFRKVETHGGFDLSGRPGSDGDEFGKVAGYGFVSGKSVHADRI
                     ATIRDVHERYGITIDTHTADGVKVAREHLTPGVPMIVLETALPAKFNETIREALGRDA
                     ERPAGFENIEALPQRFIVMPADVEQIKQTIARHTGL"
     gene            complement(5507..>6289)
                     /locus_tag="VM406_00545"
     CDS             complement(5507..>6289)
                     /locus_tag="VM406_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HVL74474.1"
                     /translation="NGITQVYQLAALLSVTGEQAPLKAWTLNMNGLLNILELARERTQ
                     AGKPLKVFWPSSIAAFGPHTPAVDTPQLAVMDPTTIYGISKQAGERLCEYYFTKFGVD
                     VRSIRYPGIISYKAPPGGGTTDYAIAIFHAARRGERYTCFLGPETTLPMIYMPDAIRA
                     TIELMEAPPEKVRIRSAYNVAGLSFNPRELAAAIRKRVPGFEIVYAPDHRQAIADTWP
                     HSLDDTHARADWGWAAETGLDGLVDDMLAHVRPQAATLHHAA"
BASE COUNT         1020 a         2139 c         1990 g         1140 t
ORIGIN      
        1 ggccaagcgc cgcggcgact ggcaaaagat gtcggagctg caggcgcacc ttttcgatct
       61 cttccttgat ctgctggctg ccatgcacct gggacttctc cgccttccag acttcctcca
      121 ggtcggcata ctcgcgttcg agcttggcga tctcgtcctc gatcagcccc agccgcttct
      181 gcgacgcctc gtcggtttcc ttcttcaccg cctcgcgctc gatcttcagc tggatcaggc
      241 gccggtcgag ccggtccatc acctccggct tggaatcgat ctcgatcttg attttcgacg
      301 ccgcctcgtc gatcaggtcg atcgccttgt cgggcaggaa gcggtcggtg atgtagcggt
      361 gcgagagctc ggctgcggca acgatcgccg ggtccgtgat ctccacgccg tgatgcactt
      421 cgtacttttc ctgcaggccg cgcaggatcg cgatcgtgtc ctccacgctc ggctcgtcga
      481 cgagaatctt ctggaaacgg cgctccagcg cggcgtcctt ctcgatgtat ttgcggtact
      541 cgtccagcgt ggttgcgccg atgcagtgca gttcgccgcg cgccagcgcc ggcttgagca
      601 tattgccggc gtcgatcgcg ccttcggcct tgccggcgcc caccatcgtg tggatttcat
      661 cgatgaagac gatggtctgc ccttcgtcct gggcgatttc cttcagcacc gacttcaggc
      721 gctcctcgaa ttcgccgcgg tacttcgcgc cggccaggag cgccgccatg tcgagcgaaa
      781 gcacacgctt ggatttcagg ctgtcgggca cctcgccatt gacgatgcgc tgcgccagcc
      841 cctcgacgat cgcagtcttg cccacgcccg gctcgccgat cagcaccggg ttgttcttcg
      901 agcgccgctg cagcacctgg atcgcgcgcc ggatttcatc gtcgcggccg atcaccgggt
      961 cgagcttgcc ggcacgcgca cgctcggtca ggtccagcgt gtatttcttc agcgcttccc
     1021 gctgcccttc cgcgtcctgc gagttcacat gggtgccgcc gcgcaccgcg tcaatcgctg
     1081 cctccagcgc cttgcgcgtg aggccgttct cacgcgctag cctcccggcc tcggacttgt
     1141 cctcggtcag ggccagcagc accatctcgc tggcgatgaa ctggtcatcg cgcttctggg
     1201 cttccttgtc cgcaagattc agcagcgcga ccgattcgcg gccgatctgc acctcgccgc
     1261 cggtccccgt gaccttcggc aggcgctcca gcgcgctttt cagtcctgcg gacaggccgc
     1321 ccacgttcac gccggcgcgc tgcagcagcg agcgcgtgcc gccgtcctcc tgtttcagca
     1381 gggccgacag gatatgaacc ggctcgatgt actgattgtc gttgccgacc gccaggctct
     1441 gggcatcggc cagcgcttcc tgcagcttgg tcgtgaattt gtcgatacgc atggaacact
     1501 ccagtcagat tcctttgcca gctaagtaag gccgggcgac ggcttttcaa gcaaggatgt
     1561 cggggcattc ggtagacggg cgcggaaccg cgctaccctg ctcaggccag ggcgcgatac
     1621 agcgcgaagc cggcagcgca catcgccagt gaaccgaaca gatgcagggc cgaatgggca
     1681 aaggcccaga gcacgtcgcc gcgctggagc agcagcagcg attcggcgga gaaggtggag
     1741 aaggtggtga ggccgccgag gaaaccggtg accacgaaca gccgccaagc gatcggcagc
     1801 ccctcatgca cggagaaaaa tgcgacggca atgccgacca tgaatccgcc ggcgagattg
     1861 gcggccagcg tgccgtgcgg cagcgtggaa tgcaccgagt taagccacag cgccagcgcc
     1921 cagcgcagcc aggcgcccag cgccgcgccg acgccgatcg ccagccagtt cattcgtcgc
     1981 cggcgaggtt ggtagagcgc aacccccatc gcgccagcgc cgcatcgtcc gacaccctgg
     2041 cgtcgaccca gcgggcgcct tgcgcggtct gctctttttt ccagaacggc gcggccgtct
     2101 tcaggtagtc catgatgaac tcgcaggcgg cgaacgcctc gccgcggtgc gcggaggtaa
     2161 cggccaccag cacgatctgg tcgagcggct ggagcgggcc gacgcggtga atcacggttg
     2221 catcgaacac cggccagcgg cgcttcgcct cggtgacgat ctcttccagc gctgtctcgg
     2281 tcattcccgg gtagtgctcg agctccatcg acgcgacgcc ctcgccgtcg ttcatgtcgc
     2341 gcaccgtgcc aataaatgcc gccaccgcgc cgactttcgc attaccggca cgcaatgccg
     2401 ccacctcggc ggaaagatcg aaatcctcga tttgcacacg caccggcatg ctagcctccg
     2461 gttaccggcg gaaagaacgc gacctcaccg ccatcggcaa tgggtgtatc ggcgccggtc
     2521 atctggtgat tccaggccat gcgcagcgcc cgcccctcgg caagcgtctc ttcccacgcg
     2581 ccgccgcgtt gccgcaggaa agcccgcacg tcgccgaccg tcctcacgtc ggccggcagt
     2641 tcaagcattt ccgcgccggt tccgagcgcc tcgcgcacgc tcgcgaagaa tcgtagctgt
     2701 atacgcatat caatctagca ggtcgctgaa cggaaggaag cgcacgatgt cgccgcgcga
     2761 gatcgtggtt ccgggcggat tgtcgaccag cccgtcgccc cacaccgtcg aggtcagcac
     2821 cgccgagccc tggttcgggt acagctccag ccctccctcg ccattgaggc gcgcgcgcag
     2881 gaactcattg cggcggtccg ccttcggcca gtcgaaatcc gcgcgcatcg catacgcgcg
     2941 cggcgccacg cgctgcacgc cctgcattcg cagcaggaac ggccgcacaa acagcaggaa
     3001 ggtcacgaag cttgagaccg gattgccggg cagtccgatg aagaaggccg aaccgaactt
     3061 gcggcgcacc tcgccgaagg ccagcggctt gcctggcttg attgcgatct gccacatgtc
     3121 caggcgccct tccgcctcga ctgccggctt gacgaaatcc gcctcgccca ccgacacgcc
     3181 gccggaggtc agcaccaggt catggcgctc ggccgcatgg cacaaggtct cacgggtggc
     3241 ttcgagcgaa tcgggcacga tgccgaaatc ggtgatctcg cagccgaggt tttccagcag
     3301 cccgcgcagc acgaagcggt tggagttgta gatcgcgccg ggcttgagcc gctcgcccgg
     3361 catcgtcagt tcgtcgccgg tgaagaacat cgccacgcgc acgcgcctga ccagcggcag
     3421 atgtgcgagg ccgaccgacg cggccaggcc cagttcctgg gcgcgcaggc gcgtgccggc
     3481 cgcaagaatg gtgctgcccg cgcgaatgtc ttcgccacga cggcggatcc actcccccgg
     3541 ctgcggcgca tggcgcacga tgaccgcatc gcccgccgta ttgcccagcg catttcccac
     3601 cacctcgcac tgctcctgca tgacgaccgc atccgcaccc tcgggaatca tcgcgccggt
     3661 gaagatgcgc gcggccgtgc ccggcaatag cggctggccg acgtgcccgg ccggaatgcg
     3721 ctgcgcgacg ggcagcgttg ccgcaccgcc cgcgcagtcg gcggcacgca ccgcatagcc
     3781 gtccatctgg gtgttgtcca tcggcgggac atccagccgc gacgtctgat ccgcggccag
     3841 cacgcggccg ttcgcctcga aggtgggcag cgtttcggta tcggtaatcc gccttgccgc
     3901 cgcaagcagc aggtcgaggg cttcggctgc gctcagcatc ggtttctttc cgctcgtctg
     3961 catcctggca gcctccacct acaaaccggt gtgccgggcg atggtctgct tgatctgctc
     4021 gacgtccgca ggcatcacga tgaaacgctg cggcagcgcc tcgatgttct cgaagccggc
     4081 cgggcgctcc gcatcgcggc cgagcgcttc gcggatggtt tcattgaact tggccggcag
     4141 cgccgtctcc agcacgatca tcggcacgcc cggcgtcagg tgctcgcgcg cgaccttcac
     4201 gccgtcggcg gtgtgcgtgt cgatcgtgat gccatagcgc tcatgcacat cgcggatcgt
     4261 cgcaatgcgg tcggcatgca ccgacttgcc cgagacgaaa ccgtatcctg cgaccttgcc
     4321 gaactcgtcg ccgtcgctgc ccggcctgcc cgagagatcg aagccgccgt gcgtctcgac
     4381 cttgcggaac agcgcgttca cgcgcgcggc gtcgcggccg agcaggtcgt agatgaagcg
     4441 ttcgaaattg gacgccttgc tgatgtccat gctaggactg gtcgtatggt aggtctccgc
     4501 cgatttgcgc acgcggtaga cgccggtgcg gaagaactcg tcgagcacgt cgttctcgtt
     4561 ggtcgcgacc acgagcttgt cgatcggcag acccatcatg cgcgcgatat gccctgcgca
     4621 aatgttgccg aaatttcccg aggggacggt gaacgacacc ttctggtcgt tgctgccggt
     4681 ggctgccaga tagccgcgga agtagtagac cacctgagcg accacgcgcg cccagttgat
     4741 cgagttgacg gtgccgatct tgtaacgcga cttgaacgca aggtcgttcg acaccgcctt
     4801 gaccatgtcc tggcagtcgt cgaacacacc ctccactgcg atgttgatga tgttcgggtc
     4861 ctgcagcgag aacatctgcg cggtctggaa cgcgctcatc ttcctgtgcg gcgagagcat
     4921 gaacacacgg atgcccttct tgccgcgcat cgcgtattct gcggcgctgc cggtgtcgcc
     4981 ggaggtggcg ccgaagatgt tcaattcggc atcctgcttg gccagcgcgt actcgaacag
     5041 attgccgagc aactgcatcg ccatgtcctt gaaggccagc gtcgggccat tggaaagcgc
     5101 ctgcaggacc agcttgccgc cgtcacgctc ctcgagcacg cgcaacggcg tgatctgacg
     5161 cgcatcctcg tccgggcgcg cgttgcggta cacctgcggg gtatacgtct tgtgtgccag
     5221 cgccttcaga tcctgctcgg gaatatccgt cgcgaacttt ttcagcacct cgaaggcgag
     5281 atccgcgtag gacagcttgc gccaggcatc cagttccgca ccgctgacct tcgggtattc
     5341 gatcggaaga tacaggccgc cgtccggcgc gagaccgccg agaagaatct gggaaaagga
     5401 ttgcgccggc gcctggccgc gagtggatag gtattgcatg tcgggtggaa tgagaagggg
     5461 ctgccaagtg gcgattatac ccgccacccg gcggctgcct gcctcgttac gcggcgtggt
     5521 gcagtgtcgc ggcctgcggc cgcacatgcg ccagcatgtc gtcgaccagt ccgtccaggc
     5581 ctgtttccgc cgcccatccc cagtccgcgc gcgcatgggt gtcgtcgagg ctgtgcggcc
     5641 aggtatcggc gatcgcctgc cggtgatccg gggcgtagac aatttcgaaa cccggcaccc
     5701 gcttcctgat ggccgcggcc aactcgcgcg gattgaagct cagtccggcc acattgtagg
     5761 cggagcggat gcgcaccttc tccggcgggg cttccatcag ctcgatggtg gcgcgtatcg
     5821 cgtcgggcat gtagatcatc ggcagcgtgg tctcgggacc gaggaagcag gtgtaacgct
     5881 cgccgcggcg cgccgcatgg aagattgcga tcgcatagtc ggtggtcccg ccgccgggcg
     5941 gcgccttgta gctgatgatg ccgggatagc ggatgctgcg cacgtccacg ccgaacttcg
     6001 tgaagtagta ctcgcacagg cgctcgccgg cctgcttgct gatcccgtag atcgtggtcg
     6061 ggtccatcac cgccagctgc ggcgtgtcca ccgccggcgt gtgcgggccg aaggcggcga
     6121 tcgacgaagg ccagaacact ttcaacggct tgccggcctg cgtgcgttcg cgcgcaagct
     6181 ccaggatgtt cagcagcccg ttcatgttga gcgtccaggc cttcagcggc gcctgctccc
     6241 cggtgacgga cagcagcgcg gcaagctggt agacctgggt gatgccgtt
//
LOCUS       DATLGF010000018         7329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_206395, whole genome shotgun sequence.
ACCESSION   DATLGF010000018 DATLGF010000000
VERSION     DATLGF010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 7329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7329
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206395"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1620
                     /locus_tag="VM406_00550"
     CDS             <1..1620
                     /locus_tag="VM406_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HVL74475.1"
                     /translation="NLYREMQELAFAHLYPLRYKTLAKAVKAARGNRRELVSKILESV
                     TNTLAAGGIRAHVQGREKTLFGIYRKMRNKHLSFSQVLDVYGFRIVVDSFPNCYVALG
                     TLHALYKPMPGKFKDYIAIPKLNGYQSLHTTLIGPYGTPVEFQIRTEEMHRVAEAGVA
                     AHWLYKENDANLTDLQQRTHAWLQSLLDIQKQTGDSAEFLEHVKVDLFPDSVYVFTPK
                     SKIIALPRGATVLDFAYTIHTDIGDQAIAARVNHEHVPLRTELRNGDIVEIVTAPSSR
                     PTPNWLTFVRTGKARSAIRHHLRTINLAESTELGKRLLTQSLAALQVDPRLSDALVER
                     LLNESSARSMDELYADIGVGKRMATLVARHIRTLMENDPHAPLPPLSEAEQQPGKLDP
                     VVITGNEGVSVQLAPCCQPIPGDAIIGQLRRDQGISVHGAECEVAKRHQAKEPDRWIE
                     LAWGGDLTRRFDCRIKVLVENERGALARVAAEIGESDANITYVTMDDDREHAMQHLRF
                     TIQVEDRIHLARLMRNVRRIPGVARILRERG"
     gene            1718..2665
                     /locus_tag="VM406_00555"
     CDS             1718..2665
                     /locus_tag="VM406_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HVL74476.1"
                     /translation="MGKIHIGISGWRYAPWRGVFYPPGLPQDRELEFASRALSSVEIN
                     GAFYSLQSPESYAAWHAATPPGFTFSVKANRFLTHILRLRNLEKPMANVLASGIFNLR
                     EKLGPLLWQLPPTMRFDAAVIESFLALLPHDTRSALTLARKRERMMHGRSRLSIDANR
                     PLRHAIEVRHPSFVDEAFVTLLRKYRVALVVADTAGKWPDYEDVTADFMYLRLHGDTE
                     LYASGYSDAALARWAERIRAWSGGAEPDDANRICTAPAPRIEGRDVYCYFDNDVKVMA
                     PFDARRLIDMLGLDAGLAPLARQEAAPGESRGRRRMRRD"
     gene            complement(2766..3314)
                     /gene="greB"
                     /locus_tag="VM406_00560"
     CDS             complement(2766..3314)
                     /gene="greB"
                     /locus_tag="VM406_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HVL74477.1"
                     /translation="MNKAFVKEGDDEDDLDATPAIPPGTRNYITPAGHKRMKDELLHL
                     IDVERPEVVRIVSWAASNGDRSENGDYIYGKRRLREIDRRIRFLTKRLDIAEVVDPSV
                     HHGSDQVFFGATVTYENQAGEEHTVTIVGIDELDPLNGKISWISPVARALTKAREGDT
                     VVLRTPSGFDELTILEVRYPPP"
     gene            complement(3325..4611)
                     /locus_tag="VM406_00565"
     CDS             complement(3325..4611)
                     /locus_tag="VM406_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVL74478.1"
                     /translation="MEHSTLVPGRHAAAHLPRIREFFLARQPILNRDGTLYAYELLFR
                     SAAAGPASVTDDLAATAAVIAHASELGMENVIGASLAFINVNAEVLFSDFVHVLPRDT
                     VVLEVLETVDVTNALITRIAHLADAGYRFALDDVVADGERLERILPLVDIIKIDVKQL
                     TQPALRALTERLKREGKRLLAEKVETPEQFRTCLDLGFDYFQGYYFAKPSVLAGRKLA
                     PAQAALVRLMVQVVSGMNTEDLERTIKQDAALGLALLRLVNALPAGSEKPVDSLGQAL
                     QLIGPRQLQRWLQILVYAEPGATGAGTSPLLMTASTRGRLLERIAETVHPGERTAADC
                     AFTVGVMSLMDVLLASTMEEALEGLPVAPEIREALLERKGPYGDMLQLAESIEHVEDD
                     APKVAALLDKLGLSAEDFNALQLEAFEWSGALAHIK"
     gene            complement(4692..5639)
                     /gene="rlmJ"
                     /locus_tag="VM406_00570"
     CDS             complement(4692..5639)
                     /gene="rlmJ"
                     /locus_tag="VM406_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HVL74479.1"
                     /translation="MLSYRHAFHAGNHADVLKHCVLIQLLRYLGQKDAPYMYIDTHAG
                     AGVYALDTGAAAKNREYETGIARLWDRKDLPPVLDEYVGLVKAMNPSGKMRYYPGSPY
                     CADRTAREQDRIRLFELHPSDSRILDENFRKREDRLVQEGRRPASRGKRVMILKADGF
                     AGLKALLPPPSRRALVLIDPPYEVKDDYRRVRDTLVDALARFATGTYAVWYPVLQRME
                     SRQLPDKLKRLPAQSWLNVTLAVGAPAPDGFGLTASGMFILNPPWTLEPMLRELMPFM
                     KQALAQDEDARFSIESGAGQPVAAARGSVSGARAGADKV"
     gene            complement(5685..6107)
                     /gene="cadR"
                     /locus_tag="VM406_00575"
     CDS             complement(5685..6107)
                     /gene="cadR"
                     /locus_tag="VM406_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Pb(II)-responsive transcriptional
                     regulator"
                     /protein_id="HVL74480.1"
                     /translation="MKIGELAKATGCEVETIRYYEREGLLPAPPRSAANYRSYGPQHA
                     ERLMFIRHCRSLGMSLDDIRALQRVQAHPGSACDAVDQMLDRKLQETKTRIEELQRLE
                     QQLQSLRNACRSHLSARECGILQNLQSAALSNECVCHH"
     gene            6193..6822
                     /locus_tag="VM406_00580"
     CDS             6193..6822
                     /locus_tag="VM406_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HVL74481.1"
                     /translation="MSAHCCHAPVATRDPKYRRALWIALVVNALMFGVEIAGGLHAGS
                     AALLADAVDFFGDAANYGVSLFVLSMALAWRARAALLKGVCMGAFGIFVLGHAGWSIS
                     TGQAPEATTMGAIGALALLANLSVAALLYAYREGDANMRSVWLCTRNDAIGNVAVLIA
                     ALGVFGTGSGWPDWGVALLMGSLALSAGISVIRQARGELARPGRHEHAH"
     gene            complement(6856..>7329)
                     /locus_tag="VM406_00585"
     CDS             complement(6856..>7329)
                     /locus_tag="VM406_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74482.1"
                     /translation="GVTVAGFWTVLAAGATANRAGAFALMVVAGGAGLALMIDASQSL
                     GQLSGALAMTCAACFVASFASARAGFGQVATGTALLLLSGLLVSAHVYAEFSLTYVVL
                     LVAGLLGGAVPALLRLGGRGKHTMFGAGAATLVAGIPVLIAIALVLRAAQESGGY"
BASE COUNT         1270 a         2416 c         2367 g         1276 t
ORIGIN      
        1 aatctctacc gcgagatgca ggaactggcg ttcgcccacc tctatccgct gcgctacaag
       61 acgctggcca aggcggtgaa ggcggcgcgc ggcaatcggc gcgagctggt gagcaagatc
      121 ctggaatcgg tcaccaatac gctcgcggcc ggcggcatcc gcgcgcacgt gcagggccgc
      181 gaaaagacgc tgttcggcat ctaccggaag atgcgcaaca agcacctgtc gttctcccag
      241 gtgctcgacg tctacggctt tcgcatcgtg gtcgacagct tcccgaactg ttatgttgcg
      301 ctgggcacgc tgcatgcgct gtacaagccg atgccgggaa agttcaagga ttacatcgcg
      361 atcccgaagc tcaacggcta ccagtcgctg cacacgacgc tgatcggccc gtatggcaca
      421 ccggtcgaat tccagatccg taccgaggag atgcaccgcg ttgccgaagc cggcgtcgcg
      481 gcgcactggc tgtacaagga aaacgatgcg aatctgacgg acctgcagca gcgcacgcac
      541 gcctggctgc agtcgctgct cgacatacag aagcaaaccg gcgattcggc cgaattcctc
      601 gagcacgtga aggtcgatct gttccccgat tcggtctacg tgttcacgcc gaagtccaag
      661 atcatcgcgc tgccccgcgg cgcaacggtg ctcgacttcg cctacacgat ccacaccgac
      721 atcggcgacc aggccatcgc cgcacgcgtg aatcacgaac acgtgccgtt gcgcaccgag
      781 ttgcgcaacg gtgacatcgt cgagattgtc accgcgccct cctcgcgtcc gacgccgaac
      841 tggcttacct tcgtgcgaac cggcaaggcg cgctccgcga tccgccatca cctgcgcacg
      901 atcaacctgg ctgaatccac cgaactcggc aagcgcctgt tgacgcagtc gctcgcggcg
      961 ctgcaggtcg atcccaggct ttccgatgca ctggtcgagc gcctgctcaa cgaatcgagc
     1021 gcccgctcga tggacgagct gtacgccgat atcggcgtgg gcaagcgcat ggcgacgctg
     1081 gtcgcgcgcc atatccgcac gctgatggaa aacgacccgc acgcgcccct tcccccgctg
     1141 tcggaggccg agcaacagcc gggcaagctg gacccggtag tcatcaccgg caatgaaggc
     1201 gtgtcggtgc agctcgcgcc ctgctgccag ccgattcctg gcgacgccat catcggccag
     1261 ctgcggcgcg atcagggcat cagtgtgcat ggcgccgaat gcgaggtggc caaacgccac
     1321 caggccaagg agccggaccg gtggatcgaa ctggcctggg gcggcgacct cacgcgtcgc
     1381 ttcgactgcc ggatcaaggt cctggtcgag aacgagcgcg gcgcgctcgc gcgcgtggcc
     1441 gccgagatcg gcgaatctga cgccaacatc acctatgtga cgatggatga cgaccgcgaa
     1501 cacgcgatgc agcaccttcg attcacgata caggtcgagg accgcatcca tctggcccgc
     1561 ctgatgcgca acgtgcgccg gattccgggc gtggcacgca tcctgcgcga gcgcggctga
     1621 gcacttcgga ttgacgcagc tcggccggct cccgcaacgc cggaacgcag cctttccatg
     1681 cgcgtcgaac aagcaaaacg cattgttcgg accgcgcatg ggcaagattc acatcggtat
     1741 ctccggatgg cgttacgcgc cgtggcgtgg cgtgttttat ccgcccgggc tcccacagga
     1801 ccgcgaattg gagttcgcct cgcgcgcgct gtcgagcgtc gaaatcaatg gcgcgttcta
     1861 ttccctgcag tcgcccgaaa gctacgcagc atggcatgcg gccacgccgc caggcttcac
     1921 gttcagcgtg aaggcgaacc gcttccttac gcatatcctg cgactgcgaa acctcgagaa
     1981 gccgatggcc aacgtcctcg cgtccggcat cttcaacctg cgcgaaaagc tcggccccct
     2041 gctctggcag cttccgccaa ccatgcgttt cgacgccgca gtcatcgaat ccttcctcgc
     2101 cctgctgccg catgacaccc gctccgcact cacactggca cgcaagcgcg agcggatgat
     2161 gcacggccgt tcgcgcctgt cgatcgacgc gaaccgtccg ctgcgccacg cgatcgaggt
     2221 gcgtcatccg agcttcgtcg atgaggcttt cgtcacgctg ctgcgcaagt accgggtcgc
     2281 gctggtggtc gccgataccg ccggcaagtg gccggactac gaggacgtga ctgccgattt
     2341 catgtacttg cgcctgcacg gcgacaccga actctatgcg agcggctact cggatgcggc
     2401 gcttgcgcgc tgggcggagc gcatccgcgc atggagcggc ggcgcggaac ccgacgatgc
     2461 gaaccgcatc tgcacggcgc cggcgccgcg gatcgagggc cgcgatgtct actgctactt
     2521 tgacaacgat gtgaaggtca tggcgccgtt cgatgcgcgg cgcctgatcg acatgctcgg
     2581 tctcgacgct ggcctcgcgc cgctggcgcg gcaggaagcc gcgccgggcg aatcgcgcgg
     2641 caggcggcgc atgaggcggg actgatcgcc gggaagccct gggaattgcc gatggcaagg
     2701 cacggcgacg cgggcagtac gagcaagaag atgcaccgac gtcagcggca ttccgtcagg
     2761 gctgcttatg gcggcgggta gcggacttcc aggatggtga gctcgtcgaa cccggacggc
     2821 gtccgaagca ccacggtgtc tccctcgcgt gccttggtca gcgcgcgagc caccggcgaa
     2881 atccagctga tcttgccgtt cagcggatcg agctcgtcga tcccgactat ggtgaccgtg
     2941 tgttcctcgc cggcctggtt ctcgtaggtc acggtggcgc cgaagaagac ctggtcgctg
     3001 ccatggtgca cgctcgggtc gaccacttcg gcaatatcca ggcgcttggt caggaagcgg
     3061 atgcgccgat cgatttcgcg caggcggcgc ttgccgtaga tgtagtcccc gttctcggag
     3121 cggtcgccgt tcgatgccgc ccaggaaacg atgcgcacga cttccggccg ctccacgtct
     3181 atcaggtgca gcagctcgtc cttcatgcgc ttgtgtccgg ccggcgtgat gtagttgcgg
     3241 gtgccgggcg ggatggccgg cgtggcgtcg agatcgtctt cgtcgtcgcc ttccttgacg
     3301 aatgccttgt tcatgcgagg tattctattt gatgtgggcg agcgcgccgc tccattcgaa
     3361 ggcttccagt tgcagcgcgt tgaagtcctc ggcggacaga ccgagcttgt ccagcagcgc
     3421 cgccaccttg ggcgcatcgt cttcaacatg ttcgatcgac tcggcaagct gcagcatgtc
     3481 gccatacggc cccttgcgct ccagcaaggc ttcgcggatt tcgggtgcca cgggcaggcc
     3541 ttctagcgcc tcctccatcg tgcttgcgag cagcacatcc atcagtgaca tcacgccgac
     3601 ggtaaacgcg cagtctgcgg cggtacgttc accggggtgc acggtctcgg caatgcgctc
     3661 gagcaagcga ccgcgggtgc tcgcggtcat cagcaaagga gaggtgccgg cacctgtcgc
     3721 acccggctcc gcgtagacga ggatctgcag ccagcgctgc aactggcgcg ggccaataag
     3781 ctgcagggct tggccgaggg aatcgaccgg cttctcggac cctgccggca acgcattgac
     3841 caggcgcagg agcgccagtc cgagcgccgc gtcctgcttg atcgtgcgct ccaggtcttc
     3901 cgtattcatg cccgacacga cctgcaccat cagccgcacg agcgccgctt gggccggcgc
     3961 gagtttgcgc cccgcaagca ccgatggctt ggcgaagtag tagccctgga aataatcgaa
     4021 tccgagatcg agacaggtgc gaaactgctc cggggtctcc actttctcgg ccagcagtct
     4081 ctttccctcg cgcttcaggc gttccgtcag cgcgcgcaat gcgggctgcg tcaactgctt
     4141 gacatcgatc ttgatgatgt cgaccagcgg aaggatgcgt tcgagccgct cgccgtcggc
     4201 gaccacgtcg tccagcgcga agcggtagcc ggcatcggcg aggtgggcga tgcgcgtgat
     4261 cagtgcattg gtgacatcga cggtttccag cacttcaagc accacggtgt cccgcggcag
     4321 gacatgcacg aagtcgctga acagcacttc cgcattgacg ttgatgaagg ccagcgaggc
     4381 gccgatcacg ttttccatgc ccagctccga cgcgtgggcg atcactgccg ccgtggcggc
     4441 gagatcgtcg gtgacggaag ccggaccggc ggccgcggag cgaaacagca gctcgtaggc
     4501 gtacagcgtg ccgtcccggt tcaggatcgg ctggcgcgcg aggaaaaact ctctgatgcg
     4561 cggcaagtgc gcagcggcgt gtcgtccggg tacgagcgtg gagtgttcca ttgacatttc
     4621 catggggacg gcgccaccag caagaggaac tgcagggaga actggagcgc cgtcaacgcc
     4681 ttgcactttt atcacacttt atccgcgccc gcgcgtgctc ctgacacaga gccgcgcgca
     4741 gcggcgaccg gctggccggc tccgctctcg atggaaaagc gcgcgtcctc atcctgcgcc
     4801 agcgcctgct tcataaacgg catcagttcg cggagcatcg gctccagtgt ccacggcggg
     4861 ttgaggatga acatgccgct cgccgtcagc ccgaatccgt ccggtgccgg cgcaccgacc
     4921 gcaagcgtca cgttgagcca gctctgggcc ggcagacgct tgagcttgtc cgggagctgg
     4981 cgcgactcca tgcgctgcag caccgggtac cagacggcgt aggtgccggt ggcaaaccgc
     5041 gccagcgcgt cgaccagcgt gtcgcgcacg cgccggtaat cgtccttgac ctcgtacggc
     5101 gggtcgatca gcaccagagc gcgccgcgac ggcggcggca gaagcgcctt gagcccggcg
     5161 aatccgtcgg ccttgaggat catgacccgc ttgccgcgcg aagccggcct gcgcccctct
     5221 tggacgagcc ggtcttcgcg cttgcggaaa ttctcgtcca ggatgcggct gtcgctcgga
     5281 tgcagctcga acaggcgaat gcggtcctgt tcccgggcgg tccggtccgc gcaatacggc
     5341 gagcccgggt agtagcgcat cttcccgctc ggattcatcg ccttgaccag gccgacatac
     5401 tcgtcgagca ccggcggcag gtccttgcgg tcccacaggc gcgcaatgcc ggtctcgtac
     5461 tcgcgattct tcgcagcagc ccccgtgtcg agcgcataca cgccggcacc cgcatgggtg
     5521 tcgatgtaca tgtacggcgc gtccttctga ccgagatagc gcagcagctg gatcagcacg
     5581 cagtgtttga gcacgtcggc gtggttgccg gcgtggaagg catggcggta gctcagcatg
     5641 ggattggcgt gcgggaaagt ttgcggcggg gcgccattgt atcgctagtg gtgacagacg
     5701 cattcattgc tgagagccgc gctctgcagg ttctgcagga tcccgcattc gcgcgcgctc
     5761 aggtgcgagc ggcaggcgtt gcgcagactc tgcaactgct gctcgaggcg ctgcaattct
     5821 tcgatccgcg tcttggtttc ctgcaacttg cgatccagca tctggtctac tgcatcgcag
     5881 gcggatccgg gatgcgcctg gacacgctgc agcgccctta tgtcgtccag cgacatcccc
     5941 agcgagcggc aatggcgaat gaacatcagc cgctccgcgt gctgcgggcc atagctgcgg
     6001 tagttcgccg cgctgcgggg cggcgcgggc aacagtccct cgcgttcgta atagcggatt
     6061 gtttcgactt cgcagccggt tgccttggcc agttctccga ttttcacgtc gcttctccga
     6121 aagtgcttga ccctatagta gctacggggt ccgtaatagg caacgatcat tcacagtaac
     6181 tggagttccc agatgtcagc gcattgttgc cacgccccgg tcgccacccg cgacccgaaa
     6241 taccgtcgcg cactctggat agcactggtc gtcaatgcgc tgatgttcgg cgtcgagatt
     6301 gccggcggcc tgcacgccgg gtcggcggca ttgctggcgg atgcggtgga tttcttcggc
     6361 gatgccgcga actatggcgt atcgctgttc gtgctctcga tggcacttgc atggcgggcg
     6421 cgcgcggcgc tgctcaaggg cgtgtgcatg ggcgcgttcg gcatattcgt gctcggccac
     6481 gccggctgga gcatctcgac agggcaggcg ccggaagcga cgacgatggg ggcgatcggc
     6541 gcgctcgcgc tgctggcaaa cctctccgtg gccgccctgc tctatgcgta tcgcgaaggt
     6601 gacgcaaaca tgcgctcggt gtggctttgc acgcgcaatg acgcgatcgg caatgtagcc
     6661 gtcctgattg ctgcgctagg cgtgttcggt accggcagcg gctggccgga ctggggcgtg
     6721 gcgctgctga tgggttcgct ggccctgtcg gccggaatca gcgtcatccg gcaggcgcgc
     6781 ggcgagctgg cgcggcccgg ccggcatgag cacgcgcatt aacccgggcg gtctcagccc
     6841 gcgcgacttt acacactagt acccgccgct ttcctgcgcc gcgcgtaaca caagagcgat
     6901 cgcgatcagc accggaatgc cggcgaccaa agtggcggcg cccgcaccga acatggtgtg
     6961 ctttccgcgt ccgccgaggc gcagcagggc cggcactgca ccgccgagca gcccggcgac
     7021 cagcagaacc acgtaggtca gcgaaaactc cgcgtagaca tgtgcgctga ccagcaagcc
     7081 ggacagcagc agcaacgccg tgcccgtggc gacttggccg aagccggcgc gcgcggatgc
     7141 aaaactggcc acaaaacaag ccgcgcaggt catcgccagt gcgccgctca gctggccgag
     7201 cgactggctg gcgtcaatca tcagcgcaag accggcccct cccgccacca ccatcagcgc
     7261 gaacgcccca gcgcggttgg ccgttgcgcc agccgcaagc acggtccaga agccggccac
     7321 ggtcacgcc
//
LOCUS       DATLGF010000019         4110 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_222304, whole genome shotgun sequence.
ACCESSION   DATLGF010000019 DATLGF010000000
VERSION     DATLGF010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222304"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VM406_00590"
     CDS             complement(<1..231)
                     /locus_tag="VM406_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HVL74483.1"
                     /translation="MSEITPNEQAGGGGEEVLDAFEVRVLAVLAEKEALTPDNYPLSL
                     NALVAGCNQLSSREPVMSLSEETVREVLDRLVQ"
     gene            complement(232..984)
                     /gene="cysE"
                     /locus_tag="VM406_00595"
     CDS             complement(232..984)
                     /gene="cysE"
                     /locus_tag="VM406_00595"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HVL74484.1"
                     /translation="MFARIREDITSIMERDPAARSAWEVLTCYPGLHAIVMHRWARGC
                     WVRDFKWLGRFISQIARWLTGIEIHPGATIGRRVFIDHGFGVVIGETAEVGDDCTIYQ
                     GVTLGGTSLHKGAKRHPTLGRGVIIGAGAKVLGGFTVGEGAKVGSNAVVVKEVPPGAT
                     AVGNPAHIVKKDAGKQREEAAARVFAAYGVTPNAEEDPLSTALHKLIDHVAAQDQQMK
                     EILVALKNAGIGCAELQEREPMDAEQLNKLVD"
     gene            complement(1071..1811)
                     /gene="rlmB"
                     /locus_tag="VM406_00600"
     CDS             complement(1071..1811)
                     /gene="rlmB"
                     /locus_tag="VM406_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HVL74485.1"
                     /translation="MKSKLLFGFHAVTARMRHDASSIEEIYVDAQRHDRRMHDLLRAA
                     EAAKVRIIHAESQRLDGMAGTRRHQGVVAKAGELSLARNLDELLDAIEGPPLLLLLDG
                     VTDPHNLGACLRVADGAGAHAVIAPKDRAVGLNATAAKVASGAADTVPYITVTNLART
                     MRELKERDIWLIGTAEDADKTLYQADFTGPAGLVMGSEGEGMRRLTRENCDLLVSIPM
                     QGAVESLNVSVASGVCLYEARRQRLAAQ"
     gene            complement(2007..>4110)
                     /gene="rnr"
                     /locus_tag="VM406_00605"
     CDS             complement(2007..>4110)
                     /gene="rnr"
                     /locus_tag="VM406_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HVL74486.1"
                     /translation="VRIVGTDRRGRPEGTIVEVLERANTHVIGRLLNENGVWIVAPED
                     KRIGQDILLSGSPGKARSGQVVSVELIEQPSRYTQPIGRIAEVLGDIDDPGMEIEIAV
                     RKYGVPHEFSAAAKKLAAKLPDTVRESDLADRVDLRDVPLVTIDGEDARDFDDAVYCE
                     PVKIGRSNGYRLLVAIADVSHYVKPNDALDADALERSTSVYFPRRVIPMLPEKLSNGL
                     CSLNPDVDRLCLVCDAVITNKGELKAYQFYPAVMHSAARLTYTEVAAILSNTKGIEAA
                     RRAPLVPHLLNLYALFQVLLQARQARGAIDFETTETYIVCNAAGKIEQILPRTRNDAH
                     RLIEECMLAANVCAADLLERHKHPGVYRVHAPPSKEKLAQLRTFLKQMGLHLGGGDSP
                     SASDYAELLPKIKLRPDAVLLQTMLLRSMQQAVYSPDNIGHFGLAYEAYAHFTSPIRR
                     YPDLLTHRAIKAILQGKRYEPKGVDTSVLNTDLSPAARRMAAEDRAAGKKRKEGELAV
                     WEALGIHCSANERRADEASRDVEAWLKCYFIRDKLGEEFTGTISGVAPFGIFVQLDSL
                     FIEGLVHVTELGADYFQYDEVRHELRGERTGIRYQLTDRVTVQVSRVDLDARKIDLRL
                     VSEPDIRTVLRNEAKRAEARHRKLSADAEPVEEVAHKPARKTGGVRAPTKTRRAAAKS
                     SKSAGKSGGRARTPRKKK"
BASE COUNT          684 a         1379 c         1282 g          765 t
ORIGIN      
        1 ctggacgagg cggtcgagca cctcgcgcac cgtctcctcc gacaaggaca tcaccggctc
       61 ccggctcgac agctgattgc aacccgccac cagagcgttc agcgagagcg gatagttgtc
      121 gggcgtgagc gcttccttct ccgccagcac tgccagcacc ctcacctcga acgcatccag
      181 cacttcctcg ccgccgcccc cggcctgttc gttcggcgtg atctcgctca ttcagtccac
      241 cagcttgttg agttgttcgg cgtccatcgg ttcgcgctcc tgcagttccg cgcaaccgat
      301 cccggcattc ttcagcgcca ctagaatctc cttcatctgc tggtcctgcg cggccacatg
      361 atcgatcagc ttgtgcagcg cggtcgacag cggatcctcc tccgcgttcg gcgtgacgcc
      421 atatgctgcg aacacgcgcg cggccgcctc ttcgcgctgc ttgccggcat cctttttcac
      481 gatgtgcgcg ggattgccca ctgcggttgc gcccggcggc acctccttga ccaccacggc
      541 attggagccg accttggccc cctcgccgac ggtgaatccg cccaggacct ttgcacccgc
      601 gccgatgatc acgccgcgcc cgagcgtggg atggcgcttg gcgcccttgt gcagcgaggt
      661 gccgccgagc gtcacgcctt ggtagatcgt gcagtcgtcg ccgacttccg ctgtctcgcc
      721 gatcaccacg ccgaaaccat ggtcgatgaa tacgcggcgg ccgatcgtcg cccccggatg
      781 gatctcgatg ccggtcagcc agcgcgcgat ctgcgagatg aagcggccca gccacttgaa
      841 gtcgcgcacc cagcatccac gggcccagcg atgcatgacg atcgcgtgca ggccggggta
      901 acaggtcaag acttcccagg cggaacgcgc agcgggatcg cgttccatga tgctggtaat
      961 gtcttcacgg atgcgggcaa acatgactga tagggaaaga ttgcagggat gggtatgtta
     1021 acgtcgtgcg gccggtcgtg ccagccgcgc ccgaccacgg ctggatgggc ttattgcgca
     1081 gccagccgtt ggcgccgtgc ctcatacagg cagacacccg aggcaacgga gacattgagg
     1141 ctttccaccg cgccttgcat cggaatcgac accagcaggt cgcagttttc gcgcgtcaat
     1201 cggcgcatgc cctcgccctc ggagcccatc accagccccg cggggccagt gaaatcggcc
     1261 tgatacaggg tcttgtctgc atcttcggcg gtgccgatca gccagatgtc gcgctccttc
     1321 agctcgcgca tcgtgcgggc aagattcgtg accgtaatgt aaggaacagt gtcggccgct
     1381 ccgcttgcca ccttcgccgc cgtggcgttc agtcccaccg cgcgatcctt cggcgcgatc
     1441 accgcatgcg caccggcacc gtccgccacg cgcagacagg cgcccaggtt gtgcggatca
     1501 gtcacgccgt caagcagaag cagcagcggc gggccctcga tcgcgtccag cagttcatcg
     1561 aggttgcggg caagcgaaag ctcccctgcc ttcgcgacca ccccctggtg gcgcctcgta
     1621 cctgccatgc catcgagacg ctggctttcg gcatggatga tgcgcacctt cgcggcttcg
     1681 gcggcacgca acagatcatg catgcgccgg tcatggcgct gcgcgtcgac gtagatctcc
     1741 tcgatcgagg atgcatcgtg gcgcatgcgg gcggtgacgg cgtggaagcc gaacaggagt
     1801 ttgcttttca tcgaatccag aaatatgagt gatttggcgc acggtgcggg cagttgcgca
     1861 gaaccgcaag ctcacgacgg gcaagggcca cggcaacgtg atcgttcctg cgcttgacgc
     1921 gctgccgccc gcaaaatcgc gggacacatg acaacggcaa ccgctccggc aacacagctt
     1981 ctcgcgttca ccggtgcgga taccgcctat tttttcttgc gcggagtccg cgcgcgtcct
     2041 ccactcttgc ccgccgattt cgaggacttg gcggcggcgc ggcgcgtctt ggtcggggcc
     2101 cgcacaccac cggtcttgcg tgcaggcttg tgggcgactt cctccacggg ctcggcatcg
     2161 gcgctcagtt tcctgtgacg cgcctcggca cgcttggcct cgttgcgcaa cacggtgcga
     2221 atgtccggtt cgctgaccag gcgaagatcg atcttgcgcg cgtccagatc cacgcggctg
     2281 acctggaccg tcacgcgatc agtcagctgg tagcggatgc cggtgcgttc gccgcgcagc
     2341 tcatgtctta cctcgtcgta ctggaagtaa tcggcgccca gttcggtgac atgaacaaga
     2401 ccttcgatga acaggctgtc gagctgcacg aaaattccga acggcgcgac cccggagatc
     2461 gtgccggtga attcctcacc gagcttgtcg cggatgaagt aacacttgag ccaggcctcg
     2521 acgtcgcgcg aagcctcatc ggcacggcgc tcgttggccg agcaatggat gccgagcgcc
     2581 tcccacaccg ccagctcgcc ttccttgcgc ttcttgccgg cagcacggtc ttccgccgcc
     2641 atgcgtcgcg cggcgggcga aaggtcggta ttgagcacgc tggtatcgac acccttgggt
     2701 tcatagcgct tgccctgcag gatcgccttg atcgcacggt gcgtcagcag atcgggatag
     2761 cggcggatcg ggctcgtgaa atgggcatag gcctcgtagg cgagcccgaa atggccgatg
     2821 ttgtcggggc tgtagaccgc ctgctgcatg gaacgcagca acatcgtctg caacaggacc
     2881 gcgtcggggc gcagcttgat cttcggcagc agctcggcgt agtccgacgc gctcggcgaa
     2941 tcgccgccgc cgaggtgcag gcccatctgc ttgaggaagg tacgcaattg cgcaagcttt
     3001 tcctttgacg gcggcgcatg cacgcggtag acgccgggat gcttgtgacg ttccagcagg
     3061 tcggccgcgc acacgttcgc cgccagcatg cattcctcga tcagccggtg cgcatcgttg
     3121 cgcgtgcgcg gcaggatctg ttcgatcttg ccggcggcat tgcagacgat ataggtctcg
     3181 gtagtttcga agtcgatcgc gccgcgcgcc tggcgcgcct gcagcagcac ctggaacagc
     3241 gcgtaaaggt tgagcaggtg cggcaccagc ggcgcacgac gcgccgcctc gatgcccttg
     3301 gtattcgaca ggatcgccgc gacctctgta taggtcagcc gcgcggccga gtgcatgacg
     3361 gccggataga actggtaggc cttgagctcg cccttgttgg tgatgaccgc gtcgcatacc
     3421 aggcacaggc ggtccacgtc cggattcagc gagcataggc cgttcgacag cttctccggc
     3481 agcatcggaa tcacgcggcg cgggaaatag acggaggtgc tgcgctccag cgcatccgcg
     3541 tcgagcgcat cgttgggctt cacgtaatgg ctgacgtcgg caatggccac cagcagccgg
     3601 tagccgttgc tgcggccgat cttcaccggc tcgcagtaga cggcgtcgtc gaaatcgcgc
     3661 gcatcctcgc catcgatggt caccagtgga acgtcgcgca ggtcgacacg atcggccagg
     3721 tcagactcgc gcaccgtatc cggcagcttt gctgcaagct tctttgccgc ggcggagaat
     3781 tcatgtggca cgccgtactt gcgcaccgca atctcgatct ccatgccggg atcgtcgatg
     3841 tcgccgagca cttcggcgat gcgcccgatc ggctgcgtgt agcgcgaggg ctgctcgatc
     3901 agctccacgc tcaccacctg cccgctcctg gccttgccgg gcgagccgct caacagaatg
     3961 tcctggccga tgcgcttgtc ctcgggcgcg acgatccaca cgccattctc attgagcaga
     4021 cggccgatca cgtgcgtgtt cgcgcgttcg agcacctcga cgatcgtccc ctccggccgg
     4081 ccacgccgat cggtgccgac gatgcgcacc
//
LOCUS       DATLGF010000020         4003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_48688,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000020 DATLGF010000000
VERSION     DATLGF010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48688"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VM406_00610"
     CDS             <1..630
                     /locus_tag="VM406_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HVL74487.1"
                     /translation="GIDTAAHRGGLRGAGSTVAVIGTGADIVYPSRNRALAHEIAAGG
                     CIVSEYPLGTPGIASNFPRRNRIISGLARGVLVVEAAARSGSLITARVAAEQGRDVFA
                     IPGSIHSPLSKGCHVLIKQGAKLVESARDVLDELGEHAPAAAPRRPEQTIAASPLLDA
                     MSFDPVMPEDLASRCGIGMDQVQAELLQLEMQGMLEVLPGGKVRRLTLA"
     gene            829..1305
                     /locus_tag="VM406_00615"
     CDS             829..1305
                     /locus_tag="VM406_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HVL74488.1"
                     /translation="MFDVLVYLYETYYRPEACPDSEALIKKLSAIGFEEEEISRALGW
                     LTDLAQATSAVASGEDQPVASSSGMRVYAQRELDVFGTEAVGFLQFLESAKVIDSVQR
                     EIIIERALAASDEAVSLDKLKVIVLMVLWSQGKEPDGLIFDELFLDDEDTEPRLLH"
     gene            1543..>4003
                     /locus_tag="VM406_00620"
     CDS             1543..>4003
                     /locus_tag="VM406_00620"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HVL74489.1"
                     /translation="MTKTLIIAEKPSVANDIAKALGGFTKHDEYFESDEYVLSSAVGH
                     LVEIAAPEEYDVKRGKWTFTHLPVIPPHFALNPIAKTESRLKVLSKLIRRKDVTALVN
                     ACDAGREGELIFRLIAQYAKAKQPIKRLWLQSMTPAAIREGFARLRDDKEMLPLADAA
                     RCRSEADWLIGINGTRAMTAFNSKEGGFYLTTVGRVQTPTLSIVVEREEKIKKFVPRD
                     YWEVRAEFICAAGVYEGRWLDTKFKKDETDPEKRAERLWSKAAAESIVAACRGKQGSV
                     TEESKPATQLSPALFDLTSLQREANARFGFSAKNTLALAQALYEKHKVLTYPRTDSRH
                     LPEDYLSTVRETLDAVAENHNYHQFAKQILNKNWIKPNKRIFDNAKISDHFAIIPTTQ
                     LPKSLSEPEQKLYDMVVRRFMAVFFPAAEFQVTTRYTEVSGHTFKTEGKVMTNAGWLA
                     IYGKEAANDEDKENAGTLVAVAPGEKVKTEKVTANGLVTKPPARYTEATLLSAMEGAG
                     KLVEDEELREAMAGKGLGTPATRAAIIEGLLNEKYLLREGRELIPTAKAFQLMTLLRG
                     LGVDELTAPELTGEWEYKLAQMERGRISREEFMREIAQMTQVIVKRAKEYNNDTIPGE
                     YATLKTPCPNCGGVVKENYRRFACTQCDFSMSKIPGSRQFEIAEVEELLEKREIGPLQ
                     GFRSKMGRPFAAILKIVRDEDINNYKLEFDFGQAQEEGEDGEGVDFSDKTPLGPCPKC
                     GSGVYELGLAYVCEKTVAKPKACDFRSGRVILQQEIVPEQMAKLLNEGRTDLLPGFIS
                     QRTRRPFKAYLVRDKNGKVGFE"
BASE COUNT          828 a         1241 c         1264 g          670 t
ORIGIN      
        1 ggcatcgata ccgccgcgca ccgaggcggc ctgcgcggcg ccggatcgac ggttgcggtg
       61 atcggcaccg gcgccgacat cgtgtacccg tcgcgcaacc gcgcactggc gcacgagatc
      121 gccgccggcg gttgcatcgt cagcgaatac ccgctcggca cccctggcat cgcatcgaat
      181 ttcccgcgcc gcaatcgcat catttccgga ctcgcacgcg gcgtcctcgt ggtcgaggcg
      241 gccgcccgtt cgggctcgct gatcaccgcg agagtggcgg ccgaacaggg acgcgacgtg
      301 ttcgccattc ccggctcgat acacagcccg ctctccaagg ggtgccacgt gctgatcaag
      361 caaggggcga agctcgtgga atcagcccgg gatgtactcg acgagttggg cgagcatgcg
      421 cctgcggcgg cgccgaggcg gccggagcaa accatcgcgg cctcgccgct tctggacgca
      481 atgagcttcg atcccgtcat gccggaggac ctggcaagcc gttgcggaat cggaatggac
      541 caggtgcagg cggaactgct ccagctcgag atgcagggaa tgctggaagt gctgccgggc
      601 gggaaagtgc gcagattgac actggcgtga aacgcgccga cacgatttgc cgctgtcatc
      661 tcccgcgttt tgcgatatgc tgtaaatgtc ctgcccgcgc ccacgcgcgg cacgacaggc
      721 cgttcttgca ggagcagcat gctgcaggca agagcatgac gagagtgtca tccatcccaa
      781 cccggcccct gcgccgcctg gcgacgtggc ctcaacggta gataaatcat gttcgacgtt
      841 ctcgtttacc tctacgaaac ctattaccgc cccgaagcct gcccggactc cgaggctctc
      901 atcaagaaac tctccgcaat cggattcgaa gaagaagaaa tctcccgcgc gctcggttgg
      961 ctcaccgatc tcgcgcaggc gaccagcgcg gtcgcgtcag gtgaagacca accggttgca
     1021 tcttccagcg gcatgcgcgt gtacgcgcag cgcgaactcg acgtgttcgg caccgaggca
     1081 gtcggcttcc tccagttcct ggaatccgcc aaggtcatcg attccgttca gcgggagatc
     1141 atcatcgagc gcgcactcgc cgccagcgat gaagccgtct cgctggataa attgaaggtc
     1201 attgtcctga tggtattgtg gagccagggc aaggaacccg acgggctgat cttcgacgag
     1261 ttgttcctcg acgacgaaga cacggaaccg cgtctgctgc actaagtctc atccccttct
     1321 tgccaacgca acccgcttcg cgtcagtccg gcgcggcggc acccctcctt gcggtgtttg
     1381 ccgcaacgcg cttatcattg gccgcaaaat tgcctgcctg ttggatattc gacagggttt
     1441 gcggaacgac aagtcctccg tgccggcgcc accggcgtac ctgtttcgct gcaagactcg
     1501 ccgtctgagt gacggcgtct ttttagagac tgcattcata ccatgaccaa gaccctcatc
     1561 atcgcggaga agccctctgt cgcgaacgac atcgcgaagg cgcttggcgg cttcacgaag
     1621 cacgatgagt attttgaatc cgacgaatat gtgttgtcgt cggcggtggg tcatctggtc
     1681 gagatcgcgg cgccggaaga atacgacgtc aagcgcggca agtggacgtt tacgcacttg
     1741 ccggtgatcc cgccgcattt cgcgctgaac ccgatcgcca aaaccgagtc gcgcctgaag
     1801 gtgctgagca agctgatccg gcgcaaggac gtgacggccc ttgtcaatgc ctgcgacgcc
     1861 gggcgcgagg gcgagctgat cttccggctg atcgctcagt atgcgaaggc gaaacagccg
     1921 atcaagcggc tgtggctgca atcgatgaca ccggcggcaa tccgcgaagg atttgcgcgc
     1981 ttgcgcgacg acaaggaaat gctgccgctg gccgatgccg ctcgttgccg cagcgaagcg
     2041 gactggttga tcggaatcaa tggcacccgc gcgatgacgg ccttcaactc gaaggaaggc
     2101 ggcttctacc tgaccacggt cgggcgggtt cagacgccga ccttgtcgat cgtggtcgag
     2161 cgcgaagaaa agatcaagaa attcgtgccg cgcgattact gggaagtgcg cgcggagttc
     2221 atctgcgcgg caggcgtcta tgaagggcgc tggctagaca ccaaattcaa gaaggacgag
     2281 accgatcccg agaagcgcgc cgagcgtctg tggagcaagg ccgctgcgga atcgatcgtc
     2341 gccgcctgcc gcggcaagca gggctcggtc accgaagagt ccaagccggc cacccagctc
     2401 tctcctgcgc tgttcgatct gacgagcctg cagcgtgaag cgaacgctcg cttcggcttc
     2461 tcggcgaaga acacgctggc gctcgcgcag gcgctgtatg aaaagcacaa ggtgctgacc
     2521 tatccgcgta ccgattcgcg ccacctgccg gaagactatc tgagcacggt gcgcgagacg
     2581 ctcgacgcgg tcgcagagaa ccacaactac caccagttcg cgaagcagat cctgaacaag
     2641 aactggatca agccgaacaa gcgcatcttc gacaatgcga agatcagcga ccacttcgct
     2701 atcatcccga ccacccagct gccgaagagc ctgtccgaac cggagcagaa gctgtatgac
     2761 atggtcgtgc gccgcttcat ggccgtgttc ttcccggccg ccgagttcca ggtcaccacg
     2821 cgctacaccg aagtctcggg ccacaccttc aagaccgaag gcaaggtcat gaccaatgcc
     2881 ggctggcttg ccatctatgg caaggaggcg gccaacgacg aggacaagga aaacgccggc
     2941 acgctagtgg cggtggcgcc cggcgaaaag gtcaagaccg agaaggtcac cgccaacggc
     3001 ctggtcacga agccgccggc acgctatacg gaagccacgc tgctctccgc gatggagggt
     3061 gccggcaagc tggtcgagga cgaggaactg cgtgaagcga tggccggcaa gggcctcggc
     3121 acgccggcta cccgtgcggc catcatcgag ggcctgctga acgagaagta cctgctgcgc
     3181 gagggccgcg agctcatccc caccgccaag gcgttccagc tgatgacgct gctgcgcgga
     3241 ctgggcgtgg atgaactgac cgcaccggaa ctgactggcg agtgggagta caagcttgcg
     3301 cagatggagc gcggcaggat cagccgcgag gaattcatgc gcgagatcgc gcagatgacg
     3361 caggtcatcg tcaagcgcgc gaaggaatac aacaacgaca cgattcccgg cgagtacgcg
     3421 acgctgaaga cgccctgccc gaactgcggc ggcgtcgtga aggaaaacta tcgacgcttc
     3481 gcctgcacgc agtgcgattt ctcgatgagc aagatcccgg gcagccgcca gttcgagatc
     3541 gccgaagtcg aggaattgct ggaaaagcgc gagatcggtc cgctgcaggg cttccgttcg
     3601 aaaatgggac gcccgtttgc ggcgatcctg aagatcgtgc gcgacgagga catcaacaac
     3661 tacaagctgg aattcgattt cggacaggct caggaggaag gcgaggacgg cgaaggcgtg
     3721 gacttctccg acaagacgcc gctcggtccc tgcccgaaat gtggcagcgg cgtgtacgaa
     3781 ctcggtctgg cctacgtgtg cgagaagacg gtcgccaagc cgaaggcctg cgatttccgc
     3841 agcggccgcg tgatcctgca gcaggaaatc gtgccggaac agatggccaa gctgctcaac
     3901 gagggccgca ccgatctgct gcccggcttc atctcgcagc gcacgcggcg tccgttcaag
     3961 gcctatctgg tgcgggacaa gaacggcaag gtcggtttcg agt
//
LOCUS       DATLGF010000021        12852 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_206534, whole genome shotgun sequence.
ACCESSION   DATLGF010000021 DATLGF010000000
VERSION     DATLGF010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 12852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12852
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206534"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..2088)
                     /locus_tag="VM406_00625"
     CDS             complement(211..2088)
                     /locus_tag="VM406_00625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL74490.1"
                     /translation="MNARRRLQAIVCSPVLWRRAWALLLCLLALAVLPAHAVTVVDEA
                     QALILREGHAPLTKTVRLPFHWDAHYRHDHGRAHLKLDLPPASAPEEALYIRRIGNTF
                     RMTVDGEPFVAMGEAGNLRHDYGKQPRLFRLPPAPPGPPRRLEIEIEAQAAHSGGLSA
                     VLLGPRDEIEEIYKDEYFLRVTLYLVVALVSAVLGTVALLIWLRQRDRLFLIYAIAEF
                     LWAFQMLDVHFDQTPLPWPWWGILVLSARGLAAMAILKFVLGVMGREKSWLGLACNAL
                     LIAMVPALALAVTGVVRELEVWMRFAVELLLLASAFHVIRHGWRSPLLERRILAYSMV
                     GIVLVLLRDLLVLVVLPYTLPSLLGVGTWANHYGHITWSRYGWMLFGFALIWVIAERM
                     RRTSAELKAANTRLAQRLAQREAELSAAYAREAEAERQRATLEERQRLMRDIHDGLGS
                     QLVGAMQLAHDSDTPRPVLTRALQDAVDNLKLTVDAMQDTEGDLAALLGALRYRLAPR
                     LKAAGVELAWEVAPLPQLAGWSIQHSRHLQMILFEAFSNLLTHAAATRASLSAHRDAD
                     RVRVALADNGRGMAVGEQAGQGLANMHARAAALGAQLSFVSDGEGTRVELCLPLQVPG
                     L"
     gene            complement(2085..2789)
                     /locus_tag="VM406_00630"
     CDS             complement(2085..2789)
                     /locus_tag="VM406_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL74491.1"
                     /translation="MNAPSAQPITVAVVEDDVRARTQLCAAIGAEPGLVLAASFERLQ
                     PALEWVREHVVDVLLTDLGLPDGSGIALIRACAQRHSGTDIMVITMFGDEASVLASIE
                     AGASGYLLKDSNPPQVGQAIRELRAGGAPMSPMIARRVLTRMRTKETLPAPARKRPVP
                     GAPGVCLTRRESETLDLIARGYTYEEAARLMSVSVSTVQTYIRGIYGKLAVSSRTQAV
                     FEAHKLGLLMEGLLRP"
     gene            2980..4734
                     /locus_tag="VM406_00635"
     CDS             2980..4734
                     /locus_tag="VM406_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74492.1"
                     /translation="MNYPKTHALCAAIAVLLAGCGGGDTAVGDAVAPTLTLSGTAATG
                     LALADSPVAAKCASGSAETTTAADGGYQLTIPAGALPCVLQVSGSADGVAVVLHSVAD
                     AGSSDGAATTAVANITPLTEMVLAQMAGTMPSELFDNFDAAAAQQITPQAVSSASQQV
                     VTALKDATGIDLGSIDPFKAPLVAATPSAPTAGNQYDQLLDALKLKVSTEALPLVVNQ
                     IAAAAAAGTEASAPTLADVMTGVERGSLDGCPVAISGRYRMIDYWGRSLVRELDFKNN
                     KFNRGDGQLFLDIVPHATQACEFRASGQVDGDTIEYDVVFGPAGAGAYVSRNVTQGRS
                     TIGYIFPVQKHALSTVVGTWSFMQSGFMPGDGVQHYLGQISFAANGAVSFCDYVPASD
                     WACVAEPDVNETITEGPDHGFRINDGGEPAPMYAYRAPNGSVTLFGSTNPSGANTQST
                     EQTSFIAYKPAPVVLPQVGTVTKYWDIVQLRLPIGGGAFQNTTDFQRDANTVTAVDTV
                     ARTFSRQRASDGRPDTLLADKPVEGMRRRVATTWDDNGTTRTISGMVSISLPGLGMAL
                     GVSDGSGTHFLSVSPVRP"
     gene            5066..7819
                     /locus_tag="VM406_00640"
     CDS             5066..7819
                     /locus_tag="VM406_00640"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HVL74493.1"
                     /translation="MFLIPHPFGARALAAALFLGLNLFTEPGLAKVPGQLPGGIEQIT
                     TVEGVTEYRLPNGLRVLLLPDDAKPLVTVNLVYLVGSRHEGPGESGMAHLLEHLVFKG
                     TPTTRDPKAQFVARGMQWNGTTSFDRTNYFATFTPDNDNLSWYIGWLADSMVNSFIAQ
                     SDLDSEMTVVRNEFERAEVRTDRVLYQTMLGAAYQWHPYGRPVIGSRSDIENVSIERL
                     QRFYRHYYQPDNAVLVVGGNFDPAAVLDMVAQAFGGIPRPARVLEPQWTTEPVQQGER
                     RITVRRVGSVPLMAVSYHAAPAGSKAYAAQMVMRQILTRVPAGRLHSALVESGLAASI
                     YDWTGQTADPGVIFLGVVLNEDSDVERVKQVLLQTLESFDEPSEAEVARAKTMILNAI
                     NRSLLDANGVAMGLTEYIASGDWRLRFALRDWIAEVTPADVAQQAKSYLVESNRTLGR
                     FIPTEQPVRAPATPRPDLNALLQDYRGREAAGAIERFEPSNLAIEGHTIKRTLPGGMK
                     LALLPRATRGERVSGTLRLHWGNLEDLTGRRQDAMFLAQMMLKGTTSMTRLELSNRLS
                     DLDSSLTVGGGISGLSFNFNAPRENLDALIDLLADVVRNPVFPQSEFEQARRSFLAQN
                     QSGRNDPSTLAWNALNRHLIRAREADPRSVLTLDQAQQLGLRANVENLARFYREFAGA
                     SHSELAIVGPVDVDHVQARLQAAFGDWKSPRDYTRIPRLPEATEPERFALMMPDKANA
                     VYAAVQPIALEEQHADVPALFAAVQMLGGRAGARLWNRLREKEGLTYGIHAGLTVGML
                     EPGGRITISSTFAPQNRERFEAIVRDELEQVLRQGFTEAELADTKESILRSRRQALVG
                     EGSVAGMLADNLYWGRTMEWREQRDQAYAALQLADVNAALRKYLDPARLSIAVAGDFP
                     QEK"
     gene            8049..8489
                     /locus_tag="VM406_00645"
     CDS             8049..8489
                     /locus_tag="VM406_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVL74494.1"
                     /translation="MQSVAHILESKRHQRVHTIAPHASVLQALTLMAEMGIGALLVME
                     NERVVGIMSERDYARKVELKGRTAKDTTVAEIMTRNVIYVRPHQTNEECMALMTEGRL
                     RHLPVMDDGKLVGLISIGDLVADIISEQQFTIEQLQHYIQGDRA"
     gene            complement(8508..9266)
                     /locus_tag="VM406_00650"
     CDS             complement(8508..9266)
                     /locus_tag="VM406_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase"
                     /protein_id="HVL74495.1"
                     /translation="MKPIAPGTVIFHRHRGYGVLTSVNLLTGWVCARFGEEVRTLDLN
                     LSHDELQHADGEPIRFRRAAPDRMPHARLMAMVRELHRAGYQSLYLYSWPKPSGLHWR
                     WHLFTGQRNWLERPWREGWYGSGADYNFNPVLGWGDQPGASAAELASLLARFDPQGLA
                     QALRHDADHTAWFAAVCEALLPNYAYTLGWDLRDGPVPEHLPVIPVRKGVPPYAGPPL
                     PWPPGWTRIRAGKRVLTAGVSPKPAGLPEPGAIP"
     gene            9502..10008
                     /locus_tag="VM406_00655"
     CDS             9502..10008
                     /locus_tag="VM406_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74496.1"
                     /translation="MNTVPPAAARSIFGNAALYERLCAATEVLARVIARWPHALDRAR
                     LVQQTGRAAEEIDAICAALVRLDVLRPFGESAPAWLLAVGPAAVTLEDAFRCALAMHG
                     AESPEPRRPARPEVDMLVMQAAIAVDQRVLLLLRQFPLDRLALAQPGKAAAPRPACVR
                     QAWAVAFG"
     gene            10438..11415
                     /locus_tag="VM406_00660"
     CDS             10438..11415
                     /locus_tag="VM406_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HVL74497.1"
                     /translation="MRKALPRFFISSLLILGLSSCGGGSDSRVEVLGTAVQQDATADE
                     GLARFNALRASLGLPQLARVSLLDHAAQTHSDYQARNNTITHHQEAGKLGYQPGWENL
                     QGRITGSGYALTAPYAIGEVISRTRNRSGAYAADELVTAIYHRFGVFEPMFTRAGVGA
                     ATSGSGDTYFTANFVSPDLREGIGRTSIVVFPMDGQQDVRRNFFSDQEVPDPVPSKNE
                     VGYPVSVHADIAYIPPGATSARLLVLDVNTFTIRARGGSPLPVQPLWRSVDELTPRSA
                     AAIVPLDVLQPSTTYDVEFAGTLSERIPSTGETLNPVPVSRSWSFTTGP"
     gene            11534..>12852
                     /locus_tag="VM406_00665"
     CDS             11534..>12852
                     /locus_tag="VM406_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVL74498.1"
                     /translation="MTQAPAPIPLFDQDHETMAAAFEASATGICFLDSEGTLLRVNGA
                     LCDMLGHAPGTLNGRPWTVLAPPSLAARGTAFLASIAPDSALTSEWALTRADGSRLNA
                     FVTVRSLVLANGTRRFMASFSDLDQRRDAEQKSLSRSNDLYRTVVEYVGEAIIIVQDG
                     RISYANPRASELSGYNNHELLDKPFYSLIHPQDLARTVDRYQRSMAGMPISGRHAQFR
                     ILRRDGAERHVESSAVRIEREGRPALLAFLSDQTERQRQDMALMKSEQRHRQVVDNAT
                     EGIVVIQDGVIVFANRRTAEMAGVSIEQLQGRPFAATLHPNDQTAAAERYWRRLQGEP
                     VPPNGVVRLHNLRTKAYVWLDVSVVMIDWEGRPATLSFLTDISARKALEDRLKQSLEE
                     RDTIVENAILGMVFLNPNGRVNWANGAMYQIFGVEPGTPTGSLERFYP"
BASE COUNT         2200 a         4421 c         4210 g         2021 t
ORIGIN      
        1 gacagctacg agctctactt cggctcggcg tttcgcaacg tgctcgcgtt cgcggcgggg
       61 cagcctgtca atctcgtggc ggcgccgggc gagtgtcgtt cagtggtgcg agcaagctaa
      121 ctatgtcgtt cccgcctgag cggggcgaca acgtaagcga catggcaacc atgttcgctt
      181 gaccgaactg cgctcgcccc gcgcgcaatc ctacagaccc ggcacctgca gcggcaggca
      241 caattccacc cgcgtgccct cgccatccga cacgaagctc agctgcgcgc cgagggcggc
      301 cgcccgggcg tgcatgttgg ccagcccctg gcctgcctgc tcgccgaccg ccatgccgcg
      361 cccgttgtcg gccagcgcca cgcgaacccg atccgcgtcg cggtgcgcgc tcaggctcgc
      421 gcgcgtggcg gcggcatggg tcagcaggtt ggagaacgct tcaaacagga tcatctggag
      481 atggcgcgaa tgctggatcg accagcccgc cagctgcggc agcggcgcga cctcccaggc
      541 cagctccacg ccggcggctt tcaggcgcgg cgcaagccgg tagcgcaagg cgcccagcag
      601 cgcggcgaga tcgccctcgg tatcctgcat cgcatcgacg gtgagcttga ggttgtcgac
      661 cgcgtcctgc agcgcgcgcg tcagcaccgg gcgcggcgtg tcgctgtcat gggccagttg
      721 catcgccccg accagctggc tgccgagacc gtcatggatg tcgcgcatca ggcgctgccg
      781 ctcctccagc gttgcgcgct ggcgctcggc ctcggcttcg cgcgcgtagg ccgcgctcag
      841 ttcggcctcg cgctgcgcca ggcgttgcgc gagcctggta ttggcagctt tcagctccgc
      901 gctggtgcgc cgcatgcgtt cggcgatcac ccagatcagc gcgaagccga acagcatcca
      961 gccgtagcgc gaccaggtga tgtggccata gtggttggcc caggtgccga ccccgagcag
     1021 cgagggcagc gtgtagggca agaccaccag caccagcagg tcgcgcaaca gcaccagaac
     1081 gatcccgacc atcgaatagg cgaggatgcg gcgctccagc agcggactgc gccaaccgtg
     1141 ccggatcaca tggaacgcgc tggcgagcag cagcagctcg accgcaaagc gcatccatac
     1201 ctcgagttcg cgtacgacgc cggtgacggc cagcgccagg gccgggacca tcgcgatcaa
     1261 cagcgcattg cacgcaaggc cgagccagct tttctcgcgt cccatcacgc ccagcacgaa
     1321 cttcaggatc gccattgcgg cgagcccgcg cgcgctcagc accaggatgc cccaccatgg
     1381 ccaaggcagc ggcgtctggt cgaaatggac gtccagcatc tggaacgccc acaggaactc
     1441 ggcaatcgca taaatcagga acaggcggtc gcgctggcgc agccagatca gcagcgcgac
     1501 cgtgccgagc accgcgctga ccagggccac gacgagatag agggtgaccc gcaagaagta
     1561 ttcgtccttg tagatttcct cgatctcatc gcgcgggccg agcaggacgg cgctcaggcc
     1621 gcccgagtgc gccgcctggg cttcgatctc gatctcgagc cggcgcggcg ggccgggagg
     1681 agcgggaggc aggcgaaaca ggcgcggctg cttgccgtag tcgtggcgca ggttgccggc
     1741 ttcgcccatt gcgacgaatg gctccccgtc gacggtcatg cggaacgtgt tgccgatgcg
     1801 gcgtatgtaa agcgcctctt ccggggcgga agcgggcggc aggtccagct tcaggtgcgc
     1861 gcgcccgtga tcgtggcggt agtgcgcgtc ccagtgaaac ggcaggcgca ccgtcttcgt
     1921 gagcggcgcg tggccttcgc gcaggatcag cgcctgcgcc tcgtccacca cggtcaccgc
     1981 atgcgcgggg aggaccgcga gcgccagcag gcagagcagc agcgcccagg cccggcgcca
     2041 caagaccggg ctgcacacga ttgcctgcag gcgtcgtcgc gcgttcaagg tcgcagcagc
     2101 ccctccatca gcaggcccag cttgtgcgct tcgaagacgg cctgggttcg cgaactgacc
     2161 gcgagcttgc cgtagattcc gcgtatgtag gtctgtaccg tggacaccga gaccgacatc
     2221 aggcgcgcgg cttcttcgta ggtgtagccg cgcgcaatca ggtcgagcgt ttcggactcg
     2281 cgccgggtca ggcagacgcc cggggccccg ggcaccggac gcttgcgggc aggcgcggga
     2341 agcgtctcct tcgtgcgcat gcgcgtcagc accctgcggg cgatcatcgg gctcatcggt
     2401 gcgccgcccg cacgcagttc ccggatcgcc tgtccgacct ggggcgggtt actgtccttg
     2461 agcagatagc cggatgcgcc ggcttcgatc gaggcgagca cgctcgcttc atccccgaac
     2521 atcgtgatca ccatgatgtc ggtgccgctg tggcgctgcg cgcacgcacg gatcagcgcg
     2581 atgccgctgc cgtccggcag gccgagatcg gtcagaagca catcgacaac atgctcgcgc
     2641 acccactcca gcgcaggctg caggcgttcg aagctggccg caaggaccag gccgggctcg
     2701 gcgccgatcg ccgcgcaaag ctgggtgcgg gcgcgcacat catcctcgac cacggcgacg
     2761 gtaatgggtt gtgcggacgg tgcgttcatg ttggcaatcg gaaacctgga tgacccgatt
     2821 ctcggcgggg ccgccgcggc cggcctcaca tgttcatgcg aggcgccttg ccgattacca
     2881 atgttgccac gcttcacatt ttcatgtgaa gcgactttta cttccgcgct cctaacatcg
     2941 ccgcgagcaa tcacgctcca tgcgaaagag ggagaaatca tgaactatcc gaagacacac
     3001 gccctgtgcg ccgccatcgc ggtgctgttg gccggctgcg gcggcggcga cacagccgtc
     3061 ggcgacgccg tggcccccac cttgaccctg tccggcaccg ccgcaaccgg cctggcgctg
     3121 gccgacagcc cggttgcggc caagtgcgcg tccggcagcg ccgagacgac caccgccgcg
     3181 gacggcggct atcagctcac gatccccgcc ggcgcgctgc cttgcgtgct gcaggtgtcc
     3241 ggcagcgccg acggcgtggc cgtcgtgctg cattcggtcg ccgatgccgg cagcagcgac
     3301 ggcgcggcca ccacggcggt cgcgaacatc acgccgctga ccgagatggt gctcgcgcag
     3361 atggccggaa ccatgccgtc cgagctgttc gacaacttcg acgcggccgc cgcgcagcag
     3421 atcacgcccc aggcggtgag cagcgcgtcg cagcaggtag tgaccgcgct caaggacgcg
     3481 accggcatcg atctgggttc gatcgacccg ttcaaggcgc cgctggtggc cgccacgccg
     3541 agcgcgccga ccgccggcaa ccagtacgac cagctgctcg acgcgctgaa gctcaaggtt
     3601 tcgaccgaag cgctgccgct ggtcgtcaac cagatcgccg cggccgccgc tgccggaacc
     3661 gaggcatcgg cgccgacgct ggccgacgtg atgaccgggg tcgagcgcgg cagcctcgac
     3721 ggctgcccgg tggccatcag cggcaggtat cgcatgatcg actactgggg ccgcagcctg
     3781 gtgcgcgaac tggacttcaa gaacaacaag ttcaaccgcg gcgacggcca gctgttcctg
     3841 gacatcgtcc cgcatgcgac ccaggcgtgc gaattcaggg ccagcggtca ggtggatggc
     3901 gacacgatcg aatacgacgt cgtgttcggc ccggccggcg ccggcgccta tgtgagccgc
     3961 aacgtcaccc agggccgctc gacgatcggc tacatcttcc cggtccagaa gcatgcgctt
     4021 tccaccgtgg tcggcacctg gtcgttcatg cagagcggtt tcatgccggg cgacggggtg
     4081 cagcattacc tcggccagat ctcgttcgcc gcgaacgggg ccgtttcatt ctgcgattac
     4141 gtgcccgcga gcgattgggc atgcgtggcg gaacccgatg tcaacgaaac catcaccgaa
     4201 ggccccgatc acggcttccg catcaacgac ggcggcgagc cggcgccgat gtacgcctat
     4261 cgggcaccaa acggcagcgt gacgctgttc ggctcgacca acccgtccgg cgcgaacacg
     4321 cagagcaccg agcagaccag tttcatcgcc tacaagccgg ccccggtcgt gctgccgcag
     4381 gtgggaaccg tcaccaagta ctgggacatc gtgcaactgc ggctgccgat cggcggcggc
     4441 gcgttccaga acacgaccga cttccagcgc gacgccaaca cggtgactgc ggtcgacacg
     4501 gtcgcccgca cgttctcgcg ccagcgcgcc agcgacggcc ggcccgacac cctgcttgcc
     4561 gacaagcccg tcgaaggcat gcgccgtcgc gttgcgacga cctgggatga caatggaacg
     4621 acccgcacca tttccggcat ggtttcgatc tcgctcccgg gcttgggcat ggcgctgggc
     4681 gtcagcgacg gcagcggcac ccacttcctg agtgtgagcc cggtacgtcc ctgagcgtat
     4741 tgacgaaagg gcagcccgcg gactgccgtt caattaaggc atcgcgattc cgtggtcgcc
     4801 ccggcgaaag ccggggtcca gcgtcttcca tgcacgtccg acgccactgg atcccggctt
     4861 tcgccgggac gaccgaaact ttggatgctt gcctgaacgg cattcgccgc ggccgtctgt
     4921 tttcctcccc tgtcgttccc tcttcacccg tcacagccgc gcataaagct gcccggctga
     4981 acctttttct cccacggaag accaagcagg gcatcggcat gcctgacggt aagatgcgcc
     5041 catcgcttcc acggagtttt cccacgtgtt cctgatcccc catcccttcg gcgcgcgcgc
     5101 gctcgccgcc gccctctttc ttggcctgaa cctgtttacc gagccgggac ttgccaaggt
     5161 gccaggccag cttcccggcg gcatcgagca gatcacgacc gtggagggcg tgaccgaata
     5221 ccgcctcccc aacggcctgc gcgtgctgct gctgccggac gatgccaagc cgctggtgac
     5281 cgtgaacctg gtctacctgg tcggctcgcg ccacgaaggt ccgggcgaga gcggcatggc
     5341 ccacctgctg gagcacctgg tgttcaaggg cacgccgacc acgcgcgacc cgaaggccca
     5401 gttcgtcgcg cgcggcatgc agtggaacgg cacgacctcg ttcgaccgca ccaactactt
     5461 cgcgacgttc acgccggaca atgacaacct gtcctggtac atcggctggc tggccgattc
     5521 gatggtcaat tccttcatcg cgcaaagcga tctcgacagc gaaatgaccg tggtgcgcaa
     5581 cgagttcgag cgcgccgagg tgcgcaccga ccgcgtgctg taccagacca tgctcggcgc
     5641 cgcctaccag tggcacccgt acggtcgccc cgtgatcggc tcgcgctcgg atatcgagaa
     5701 cgtgtcgatc gagcgcctgc agcgcttcta ccgccattac taccagccgg acaacgcggt
     5761 gctggtggtc ggcggcaact tcgacccggc cgccgtgctc gacatggtcg cgcaagcctt
     5821 cggcggcatc ccccgcccgg cgcgcgtgct cgagccgcaa tggaccaccg agccggtgca
     5881 gcagggcgag cgccgcatca ccgtgcgccg cgtcggcagc gtgccgctga tggcggtcag
     5941 ctatcacgcg gcgccggccg gcagcaaggc gtacgcggcg cagatggtga tgcgccagat
     6001 cctcacgcgc gtgccggcag ggcgcctgca cagcgcactg gtcgagtccg gcctggccgc
     6061 atcgatctac gactggaccg gccagaccgc ggacccgggc gtgatcttcc tcggcgtggt
     6121 cctcaacgag gattcggacg tcgagcgcgt gaagcaggtc ctgctccaga cgctggaatc
     6181 cttcgacgag ccgagcgagg cggaagtcgc gcgcgccaag accatgatcc tgaatgcgat
     6241 caaccgctct ctgctcgatg ccaacggcgt cgcaatgggc ctgaccgaat acattgcctc
     6301 gggcgactgg cgcctgcgct ttgcgctgcg cgactggatc gccgaagtca ccccggccga
     6361 cgttgcccag caggcgaaaa gctacctggt ggagagcaac cgcacgctag gccgcttcat
     6421 tccgaccgag cagccggtgc gcgcgccggc caccccgcgc cctgacctga acgcactgct
     6481 gcaggactac cgcggacgcg aagccgccgg tgcgatcgag cgcttcgagc cgagcaacct
     6541 ggcgatcgag gggcatacga tcaagcgcac gctgcccggc ggcatgaagc tcgcgctgct
     6601 gccgcgcgcc acgcgcggcg agcgcgtgtc cgggacgctg cgtctgcact ggggcaatct
     6661 cgaggacctg accggccgcc ggcaggacgc gatgttcctc gcacagatga tgctgaaagg
     6721 cacgaccagc atgacccggc tggagctgag caaccgcctg tccgacctcg acagcagcct
     6781 gacggtcggc ggcggcatca gcggcctttc cttcaatttc aatgcgccgc gcgagaacct
     6841 cgacgcgctg atcgacctgc tcgccgacgt ggtgcgcaac ccggtcttcc cgcaaagcga
     6901 attcgagcag gcgcgccgca gcttccttgc acagaaccag agcgggcgca atgatccgtc
     6961 cacgctggcc tggaatgcgc tgaaccggca cttgatccgg gcccgggagg ccgatccgcg
     7021 cagcgtgctc acgctggacc aggcccagca gctgggcctg cgcgcgaacg tggagaatct
     7081 tgcacgcttc taccgcgaat ttgccggcgc cagccatagc gagctggcca tcgtcggtcc
     7141 ggtcgacgtc gatcatgtcc aggcccgcct gcaggccgcg ttcggcgact ggaagagccc
     7201 gcgcgactac acccgcattc cgcgtctgcc cgaggcgacc gagccggagc gcttcgcgct
     7261 gatgatgccg gacaaggcca atgcggtcta cgcggcggtg cagccgatcg cgctggagga
     7321 acagcatgcc gacgtgcccg cgctgtttgc cgctgtgcag atgctgggcg gccgagccgg
     7381 cgcgcgcctg tggaaccggc tgcgtgaaaa ggaggggctg acgtacggca tccatgccgg
     7441 cctcaccgtc ggcatgctcg agccgggcgg gcgcatcacg atcagcagca ccttcgcgcc
     7501 gcagaaccgc gaacgcttcg aggcgatcgt gcgcgacgaa ctggaacagg tgctgcgcca
     7561 gggcttcacc gaggccgaac tggccgacac gaaggaatcg attctgcgca gccgccgcca
     7621 ggcgctggtg ggcgaaggct cggtcgccgg catgctggcc gacaacctgt actggggacg
     7681 cacgatggaa tggcgcgagc agcgcgacca ggcttacgcg gcgctgcaac ttgccgatgt
     7741 gaacgccgcg cttcgcaaat acctggaccc ggcccgcctg tcgatcgccg tcgccggtga
     7801 cttcccgcaa gaaaaataaa caacgctcgc ggcagccggt tccggccgct tgcggcactg
     7861 cgaaaaagcg ccgacactgg atgcgtcggg acgactcccc gtcgtcccgg cgcagccgct
     7921 ccgggcggca cggttcatcc ggccggactg cggctcctgc acgcagtgag ctgccagaca
     7981 tgccggcccg cgcggcccgc aggtccggca ttgttcacgc accttgccac cagccacggg
     8041 agacacgtat gcagtcggtc gcacatatcc tcgaatccaa gcggcaccag cgcgttcaca
     8101 ccattgcccc gcatgcctcg gtactgcagg cgctgaccct gatggcagag atgggtatcg
     8161 gcgccctgct cgtgatggaa aacgaacggg tggttggcat catgtccgag cgcgactacg
     8221 cccggaaggt tgaattgaag ggtcgtacgg cgaaggatac gacggtggcg gagatcatga
     8281 cgcgcaacgt gatctacgtg cgcccgcacc agaccaacga ggaatgcatg gcgctgatga
     8341 ccgaggggcg gctgcgtcat ctcccggtga tggacgacgg caagctcgtc ggcctgatct
     8401 cgatcggcga tctggtcgcc gacatcatct cggaacagca gttcacgatc gaacagctgc
     8461 agcactacat ccagggcgac cgcgcgtaag cgcggacatg cggcccctca gggtatggcg
     8521 ccgggctcgg gaagccctgc cggcttcggg ctgactccgg ccgtcagcac gcgcttgccg
     8581 gcccggatgc gcgtccagcc ggggggccac ggcagcggcg ggcccgcgta gggcggcacg
     8641 cccttgcgca cgggaatcac cggcagatgt tccggcaccg gcccgtcgcg cagatcccac
     8701 cccagcgtat aggcgtagtt cggcagcagc gcctcgcata cggcggcgaa ccaggccgta
     8761 tggtcggcgt cgtggcgcag tgcctgcgcc agaccctgcg ggtcgaagcg tgccagcagg
     8821 ctggcgagtt cggccgcgct tgcgcctggc tggtcgcccc agccgagcac tgggttgaag
     8881 ttgtagtccg cgccggagcc ataccagcct tcgcgccacg gccgctccag ccagttgcgc
     8941 tgtccggtga acagatgcca gcgccagtgc aggccggagg gcttgggcca gctgtacagg
     9001 tacaggcttt gataacccgc gcggtgcagc tcccgcacca tcgccatcag gcgcgcatgc
     9061 ggcatccggt cgggggccgc gcggcgaaag cgtatcggct ccccgtcggc gtgctgcagt
     9121 tcatcgtgcg aaaggtttag atccagggtg cgcacttcct cgccgaagcg ggcacagacc
     9181 cagccggtca gcagattgac cgacgtgaga acgccatagc cgcggtggcg gtgaaagatg
     9241 acggttccgg gagcgatcgg tttcatggag tctggcagtg tacgggattg gcccggcagc
     9301 gggcaaaaag aacggcgcac ctgcgtgcac cgcgtattgc gttgtcgtca ggcaccggac
     9361 cggtgcacgt tgtcccggat taacccgggg caggccgttt tgaggcgtaa accgcaagga
     9421 aattccctgt cgccgcgtga gttgactggt ccgcttcttg caatgttgcc ggcttgcatc
     9481 gccaatcccg gagccaccag catgaacacc gtcccgccag ccgccgcgcg cagcatcttc
     9541 ggcaacgccg ccctctacga acgcctgtgt gcggccaccg aagtgctggc ccgcgtcatc
     9601 gcgcgctggc cgcatgcact cgaccgcgcg cggctggtgc agcagaccgg tcgagccgcg
     9661 gaagagatcg acgcgatctg cgcggcgctc gtccggctgg acgtgttgcg gccattcggg
     9721 gaaagcgcgc cggcctggct gttggccgtc ggacccgccg ccgtcacgct cgaggatgcg
     9781 ttccgctgcg cgctggccat gcacggcgcg gaatcccccg agccgcgccg gcctgcgcgt
     9841 cccgaagtcg acatgctggt catgcaggcg gcgatagcgg tcgaccagcg cgtcttgctg
     9901 ctgctgcgcc agtttccgct ggaccggctc gcactggctc agcccggcaa agcggcggcg
     9961 ccccggcccg cctgcgtcag gcaggcatgg gccgtggcat ttggctgagc gggaggggac
    10021 tgacgctcgg tcaaggctat tgatggacgg tcagtcgatg cgccgaaagc ataacgttat
    10081 gccaacattg cattggctgc cggtgtggca gttttattaa gattgcctgg cagtgaacga
    10141 agttggtccc ggtcactgct tttccaaggg gttggggagt gtcgtttcgg cgccactccc
    10201 tttttttatt gtgccgcggc tgcggttgcg cgcgcgtggg gtaccgatct agcacatccg
    10261 atcctaagcc cggagccttc cggagaagtt gagaagccgc aaaacgttgt attccgcaca
    10321 cgccaagatg actgccaaca ggcaaagcga tatgctgccg atgtgcaaca aaatgtaaat
    10381 caggcgtaac actttcggcc cgctaccggg gccgcgccag gcacaaagga ataccgaatg
    10441 cgcaaggctc tgccccgatt ttttatctcg tcccttttga ttctcggcct ctccagctgc
    10501 ggcggcggct cggacagccg ggtcgaggtg ctgggcacgg ccgtccagca ggatgcgact
    10561 gccgatgaag gattggctcg cttcaatgcg ctgcgcgcca gcctcgggct gccccagctc
    10621 gcgcgggttt ccctgctcga ccacgcggcg cagacgcatt ccgactacca ggcccgcaac
    10681 aacaccatca cccaccatca ggaggccggc aagctcggct accagcccgg atgggaaaac
    10741 ctgcagggtc gcattaccgg ctccggctac gcgctgacgg cgccgtatgc gatcggcgaa
    10801 gtgatctcac gcacgcgcaa ccggtccggt gcgtacgcgg ccgacgaact ggtcaccgcg
    10861 atctaccacc gcttcggggt attcgagccg atgttcaccc gcgccggcgt cggtgccgcg
    10921 acctcgggct ccggcgacac ctacttcact gcgaacttcg tcagccccga tctgcgcgag
    10981 ggaatcggcc gcaccagcat cgtcgtgttt ccgatggacg ggcagcagga tgtgcgtcgc
    11041 aacttcttca gcgatcagga agtcccggac ccggtgccgt cgaagaacga agtcggctat
    11101 ccggtcagcg tccatgccga cattgcctac attccgcccg gcgcgaccag cgcgcgtctg
    11161 ctggtgctcg atgtgaacac gttcacgatc cgtgcgcgcg gcggttcccc tttgcccgtg
    11221 cagccgctgt ggcgcagcgt ggacgagctc acccccagat cggccgccgc catcgtgccg
    11281 ctcgacgtgc tccagccatc caccacctat gacgtggaat tcgccggcac gctttccgag
    11341 cgcatcccgt ccaccggcga gaccctcaat ccggtgccgg tgagccgcag ctggagcttc
    11401 accaccggtc cctgagtttt ttacttcctg ttacacgccg gctgctcttc tgctatcttc
    11461 attgctggga gacatgaaga accggcccgc cgcgtcgaaa acggcaggcg ccgataacaa
    11521 gtagaagaaa cgcatgacgc aggcgcccgc gcccattccg ttgttcgacc aggaccacga
    11581 aacgatggcg gccgcgttcg aggccagcgc caccgggatc tgctttctgg acagcgaggg
    11641 aacgctgctc agggtgaacg gcgccctgtg cgacatgctc ggccatgcgc ccggaacgtt
    11701 gaacggaagg ccatggaccg tgctcgcgcc gcccagcctg gccgcgcgcg gcacggcctt
    11761 cctcgcgtcg atcgccccgg attccgccct gaccagcgag tgggcgctta cgcgcgccga
    11821 cggctcgcgc ctgaacgcct tcgtcaccgt ccgttccctc gtgctggcga acggcacgcg
    11881 ccgcttcatg gcgagctttt ccgacctcga ccagcgccgc gacgccgaac aaaagagcct
    11941 gagccgctcg aatgacctgt accggacggt cgtcgaatac gtcggcgagg cgatcatcat
    12001 cgtgcaggac ggccgcatca gctatgccaa cccgcgcgca tccgagcttt cgggctacaa
    12061 caaccacgag ctgctcgaca agccgttcta cagcctgatc catccgcagg atctggcaag
    12121 gacagtcgac cgctaccagc gcagcatggc cggcatgccg atctccggac gccacgccca
    12181 gtttcgcatc ctgcgccgcg acggggcgga gcgccatgtg gagtcgagcg ccgtgcgcat
    12241 cgagcgggaa gggcgtccgg cgctgttggc cttcctttcc gaccagacgg agcgccagcg
    12301 ccaggacatg gcgctgatga agtccgagca gcgccaccgg caggttgtcg ataacgccac
    12361 cgaagggatc gtggtcatcc aggacggagt gattgtcttc gccaaccggc gcacggccga
    12421 gatggccggc gtgtcgatcg aacaattgca gggccggccg ttcgcggcga ccctgcaccc
    12481 gaacgaccag accgccgccg ccgagcgcta ctggcgccgg ctgcaaggcg agccggtgcc
    12541 gccgaacggc gtagtgcgac tgcacaatct gcgcacgaag gcatatgtct ggctggatgt
    12601 ctcggtcgtc atgatcgact gggagggccg gccggccacg ctgtccttcc tgaccgacat
    12661 cagcgcgcga aaggcgctcg aggaccggct caagcaaagc ctggaagagc gcgacacgat
    12721 cgtcgaaaac gccatcctcg ggatggtttt cctgaacccc aacggcaggg tgaactgggc
    12781 caacggcgcg atgtaccaga tcttcggcgt cgagcccggc acgccgacag gctcgctgga
    12841 gcgcttctat cc
//
LOCUS       DATLGF010000022         9661 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_64033,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000022 DATLGF010000000
VERSION     DATLGF010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 9661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9661
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64033"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VM406_00670"
     CDS             complement(<1..423)
                     /locus_tag="VM406_00670"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HVL74499.1"
                     /translation="MTSPPSKRRSRAWFEKQDRDGFVHRSWTAKPGYPPDAFDGRPVI
                     GICNTWSELTPCNSHFRELAEFVKRGVLEAGGFPLEFPVMSLGETQLRPTAMLFRNLA
                     SMDVEESIRGNPMDGVVLLVGCDKTTPALLMGAASCGLP"
     gene            514..1290
                     /locus_tag="VM406_00675"
     CDS             514..1290
                     /locus_tag="VM406_00675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL74500.1"
                     /translation="MSEERGLAIFPSLEGRRVFITGGGSGIGAALVMAFAEQRAQVAF
                     VDIADAASRELCERIAGEGWPRPLFHLCDIRDVAALQQSIADFAAEIGEFEVLVNNAG
                     NDQRHRPEEVTPAFWDNCIAINQRAMFFACQAVIEGMKRRGEGSIINLGSTSWHIANG
                     GYPVYTTAKASVSGLTRGLARDLGQYGIRVNTVTPGWVMTERQLALWVDEEGEKLIER
                     SQCLKGRVMPWHVARMVLFLASHDAAMCSAQEFIVDGGWA"
     gene            1294..2205
                     /locus_tag="VM406_00680"
     CDS             1294..2205
                     /locus_tag="VM406_00680"
                     /inference="COORDINATES: protein motif:HMM:NF016894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HVL74501.1"
                     /translation="MGAELIVGIDWGTSNRRAYVLDARGALLRRHEDAMGILSVEGDY
                     RGSLQALLARLEVERGEVLMSGMVGSRNGWHQVPYLSTSAPLTALAEALFEVPDAPQG
                     LRCRIVPGYSHVDSNGLPDVMRGEETQLLGALALGAEQGASGWFLLPGTHSKWANVDN
                     GRIVEFFTYMTGELYALLTTHGTLARVAQADESVSEAFAAGLQAARHGSFSHLAFCCR
                     ALVVTDAMPASHTTSWLSGLLIGSELYDILRRVPSGMPQTVQVVGSPALARRYMAALE
                     LLGIPARTWEPDEVYLAALRILAGLPR"
     gene            2211..2837
                     /locus_tag="VM406_00685"
     CDS             2211..2837
                     /locus_tag="VM406_00685"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HVL74502.1"
                     /translation="MELFQPSPCPLVAILRGLTPAEAPEVGRILFESGFRILEVPLNR
                     PQAIEAMRELLRIAPDDALVGAGTVLTIEDVEAVRAAGGRLIVSPHCAVEVISHAVKS
                     GMIAMPGVTTPTEAYSALGAGAQGLKLFPAEMIPPAAVKSIRSILPPGTPLFPVGGIT
                     PQQMAPYVAAGATGFGIGGQLYRPQVEAGALKRAAAAFMSARKLILQG"
     gene            2974..4572
                     /locus_tag="VM406_00690"
     CDS             2974..4572
                     /locus_tag="VM406_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HVL74503.1"
                     /translation="MLSTANITMQFGPKPLFENISVKFGDGNRYGLIGANGCGKSTFM
                     KILGGDLEPTGGTVMLDPNERLGKLRQDQFAFEEMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATDDDYMRAADLEAKFAEYDGYTAEARAGELLLGVGVPTEQHNGPMSNVAPGW
                     KLRVLLAQALFSNPDILLLDEPTNNLDINTIRWLENVLNERNSTMIIISHDRHFLNQV
                     CTHMADMDYGTLKIYPGNYDDYMLASAQARERQAAANAKAKERVAELQDFVRRFSANK
                     SKARQATSRLKQIDKIKIDDFKPSSRQNPFIRFEGEKKLHRQAVEVEGLTKSYDRTIF
                     KNINLMVEAGEKIAIVGANGAGKTTLLRCIGAAACAMEPDSGKVKWAEHANIGYMPQD
                     PTEEFATDMNLTDWMGRWTQEGDDDQAVRSILGRLLFSGDDVKKSVKVLSGGEKGRMM
                     YGKLMLGRHNVLLLDEPTNHMDMESIESLNIALDKYAGTLIFVSHDREFVSSLASRIL
                     EVKEGEVIDFHGGYEEYLASQGIE"
     gene            complement(4603..4842)
                     /locus_tag="VM406_00695"
     CDS             complement(4603..4842)
                     /locus_tag="VM406_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74504.1"
                     /translation="MEDPLYQALYRFYTDTIRRNLGLRGHTLQMRVELARSSVELLAL
                     REAWLAALWQARGNDVARALRHELEMLLRRSRQPA"
     gene            5096..6235
                     /gene="nadA"
                     /locus_tag="VM406_00700"
     CDS             5096..6235
                     /gene="nadA"
                     /locus_tag="VM406_00700"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HVL74505.1"
                     /translation="MQTLEIKPVEFELPRMDAGTSCTASAWARVPAAPSPQEKAALKE
                     RIRRLLKDRQAVLVAHYYVDADLQDLAEETGGCVSDSLEMARFGRDHPARTLVVAGVR
                     FMGETAKILSPEKTVLMPDLDATCSLDLGCPADEFAAFCDAHPDRTVVVYANTSAAVK
                     ARADWMVTSSIGLQIVEHLHAQGKKILWAPDKHLGGYIQKKTGADMLLWQGSCIVHDE
                     FKGIELELLKKEHPGAKVLVHPESPEAVVAQADVVGSTSQLIAAARTLDAQEFIVATD
                     NGILHKMKMAAPGKTFIDAPTAGNSATCKSCAHCPWMAMNGLQNLADVLETGRNEIHV
                     DPEIGRKAFACIDRMLDFAAKQKANVRPGSDLAKEQQLFKGIGPA"
     gene            6232..7098
                     /gene="nadC"
                     /locus_tag="VM406_00705"
     CDS             6232..7098
                     /gene="nadC"
                     /locus_tag="VM406_00705"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HVL74506.1"
                     /translation="MSTLKNPFAPFDAALTAAFEANIQAALNEDVGSGDLTGLLVPED
                     EQVKARVVVREAAVLCGAPWFEAVMKQLDARMRIQWHYAEGDMMRADSEVCTIDGPAR
                     ALLTAERTALNFLQLLSGVASATRRYVDAIAGTRSAILDTRKTLPGLRLAQKYAVRVG
                     GGQNQRLALYDGILIKENHIAAAGGIAPAMKAAFALDAGVSIQIEVESLEELEQALAA
                     GASSILLDNFSLDLMREAVRITAGRAVLEASGGVNMETVRAIAETGVDRISIGSLTKD
                     VRATDYSLRILA"
     gene            7142..8041
                     /locus_tag="VM406_00710"
     CDS             7142..8041
                     /locus_tag="VM406_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140892.1"
                     /note="3' incision activity; acts with UvrC; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HVL74507.1"
                     /translation="MAEHLDAFRRMPALVTSVGLFKPDPADSFTYPDHIDPDSLAALP
                     SAPGVYIFLDERERPLYIGKSVNVRSRVLSHLRTPEEARMLQQTRRIDFHRTAGEIGA
                     LLLESRLIKDRQPVHNKKLRRAREMCALRIANGVPEIVHARDHDFASEHGLYGLFGTR
                     RAALERLRRLVEEQRLCPVLCGVEPAPKAGRACFACQVGRCAGACVGRESRDAHARRL
                     AGALEDLRIVTWPFPGPVGIIERCEGWRQMHVVDRWFYLGTVDQPRRMRAPAQKGRPR
                     FDVDTYSILVRPLLLGELELRML"
     gene            complement(8118..8678)
                     /locus_tag="VM406_00715"
     CDS             complement(8118..8678)
                     /locus_tag="VM406_00715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HVL74508.1"
                     /translation="MIRIAIIVGSTRPGRKADAVANWAYEIARQRNDAQFEVVDIQDF
                     NLPLLDEPVPPSMDQYSKPHTKAWADCIGSFDAFVFVTPEYNHGTSGALKNAIDFLYK
                     EWNNKAAGFVGYGSAGGVRAVESLRLVMGELQVADVRAQVMLSLSSDFENYTTFKPDQ
                     CHEATLGTMLDQLIAWGNALKTVRQA"
     gene            8777..8977
                     /locus_tag="VM406_00720"
     CDS             8777..8977
                     /locus_tag="VM406_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HVL74509.1"
                     /translation="MQRVLIAAGVLLLIIGVAWPWVSRLPLGRLPGDIHIVREGFSFY
                     FPIVTCIVISIVVTLLFWIFRR"
     gene            complement(9104..>9661)
                     /locus_tag="VM406_00725"
     CDS             complement(9104..>9661)
                     /locus_tag="VM406_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HVL74510.1"
                     /translation="VVASEVRSLAQRSAAAAKEIKALIDNSVEKVDTGARLVNEAGST
                     MHEIVDSIKRVADIMGEITAASAEQTSGIEQVNQAIAQMDQTTQQNASLVEEAAAAAE
                     AMQDQARQLSQAVSVFRIEANQAVTLAPVAPKPVRPAAAVAVPSKPVKPAVPAAAPGT
                     TERSVAPIPRRPAPPPGKGDDWEEF"
BASE COUNT         1660 a         3074 c         3287 g         1640 t
ORIGIN      
        1 cggcagcccg cagcttgcag cgcccatcag cagcgcgggc gtggtcttgt cgcagccgac
       61 caggagcaca acgccgtcca tcggattgcc gcggatcgat tcctcgacgt ccatgctcgc
      121 gaggttgcga aacagcatcg cggtcggccg aagctgcgtt tccccaaggg acatcaccgg
      181 gaactcaagc gggaatccgc ctgcctcaag tacaccgcgc ttgacgaatt cggccagctc
      241 gcggaagtgg ctgttgcagg gcgtgagttc ggaccacgtg ttgcagatgc cgatcacagg
      301 gcggccgtcg aaggcgtcgg gcggatagcc gggcttggcg gtccagctgc ggtgcacgaa
      361 gccgtcgcgg tcctgctttt cgaaccaggc gcggctgcgg cgtttggatg ggggggatgt
      421 catgcgtgcg tcccggaaag agatcggtgc gccggacgga aattccgggc tccaatatgg
      481 attggggtgc tgtttgaaag ttcaggagag cgcatgagcg aggaacgcgg actggcgatc
      541 tttcccagcc tggagggcag gcgggtgttc atcaccggtg gcggctccgg catcggcgcg
      601 gcgctcgtca tggcattcgc cgagcaacgc gcgcaggtcg ctttcgtcga catcgccgac
      661 gcggccagcc gcgagctgtg cgaacggatc gctggcgaag gctggcccag gcccttgttc
      721 catctttgcg atatccgcga tgtggcggcg ctgcagcaga gcatcgccga tttcgcagcc
      781 gagatcggcg agttcgaggt gctggtcaac aatgccggca acgaccagcg ccaccgcccc
      841 gaggaagtca cgccggcgtt ctgggacaac tgcatcgcga tcaaccagcg cgcgatgttc
      901 ttcgcctgcc aggcggtgat cgaaggaatg aagcggcgcg gtgaaggttc catcatcaat
      961 ctgggatcga ccagctggca tatcgccaac ggcggctatc cggtctacac gaccgcgaag
     1021 gcctcggtgt ccggcctcac ccgcggcttg gcgcgcgatc tcggccagta cggcatccgc
     1081 gtgaacacgg tgacaccggg atgggtgatg accgaacgcc agctggcgct gtgggtcgac
     1141 gaagagggcg agaagctgat cgagcgcagc cagtgcctga agggcagggt gatgccctgg
     1201 cacgtggcgc gcatggtgtt gtttctggcc tcgcatgacg cggccatgtg cagtgcacag
     1261 gaattcattg tcgatggagg atgggcatag cggatgggcg cagagctgat cgtcgggatc
     1321 gattggggaa cgagcaatcg ccgggcctat gtgctcgacg cgcgtggcgc gctgctgcgg
     1381 cggcacgagg acgcgatggg catcttaagc gtggagggcg attaccgtgg ctcgctgcag
     1441 gcgctgctcg cccggctcga ggtcgagcgc ggtgaagtgc tcatgtccgg gatggtcggc
     1501 agccgcaacg gctggcacca ggtgccctat ctgtcgacca gcgcgccgct gaccgcactc
     1561 gccgaagcgc tcttcgaggt gccggacgcg ccgcagggcc tgcgctgccg catcgtgccg
     1621 ggctacagcc acgtcgactc caacgggttg ccggacgtga tgcgtggcga ggaaacccag
     1681 cttctcggcg cgctcgcgct cggtgccgag cagggcgcgt ccggctggtt cctgctgccg
     1741 ggcacgcaca gcaagtgggc aaacgtggac aacgggcgca tcgtcgagtt cttcacctac
     1801 atgaccggcg agctgtatgc gttgctgacg acgcacggaa cgcttgcacg cgtggcgcag
     1861 gcggacgagt cggtgtcgga ggccttcgcc gccggcctgc aggcggcgcg ccacggcagc
     1921 ttcagccacc tcgcgttctg ctgccgcgcg ctggtggtga ccgacgcgat gccggccagc
     1981 catacgacgt cctggctgtc gggcctgctg atcggcagcg aactgtacga catcctcagg
     2041 cgcgtgccga gcggcatgcc gcagacggtg caggtggtcg gaagcccggc gctggcgcgc
     2101 cgctacatgg ccgcactcga gctgctcggc attcccgcgc gtacctggga gcccgacgag
     2161 gtttatctgg cggccctgcg cattcttgcc gggctgccgc gttgacggat atggaactct
     2221 ttcaaccttc accatgcccg ctggtcgcga tcctgcgcgg gctcacgccg gccgaagcac
     2281 ccgaggtcgg acgcatcctg tttgagtccg gctttcgcat cctcgaggtg ccgctgaacc
     2341 gcccgcaggc gatagaagcc atgcgcgaac tgttgcgcat cgccccggac gatgcgctgg
     2401 tcggcgccgg cactgtgctc accatcgaag atgtcgaagc ggtgcgcgcg gccggcgggc
     2461 ggctgatcgt gtcgcctcat tgcgcggtcg aagtgatatc ccacgcggtc aagtccggca
     2521 tgatagcgat gcccggggtc accacgccga ccgaagccta ttcggcgctg ggtgccggcg
     2581 cgcaggggct gaagctgttt cccgcggaaa tgattccacc ggcggcggtg aagtcgatcc
     2641 gctcgatcct gcctcccggt acgccgctgt tccccgtggg cggcatcacg cctcagcaga
     2701 tggcgccgta tgtcgccgcc ggcgccaccg gtttcggcat tggcggccag ctctaccgcc
     2761 cgcaggttga ggcgggggcg ctgaaacggg ctgccgcggc attcatgtcg gcccgcaagc
     2821 tgatcctgca gggctgaagc tgctcgaccg gggtgataaa atgcccggtt ttcccgaatg
     2881 ccaaaaggga atgccaaagg gcccgccgac acggtcaggc cgcttcgcaa cggcggcatt
     2941 cgggcaccct gctcatcctg gaaggttccg cacgtgctgt ccactgccaa tatcaccatg
     3001 cagttcggcc ccaagccgct gttcgaaaac atctccgtca agttcggcga cggcaaccgc
     3061 tacggtctga tcggcgcgaa tggctgcggc aagtcgacgt tcatgaagat cctcggcggc
     3121 gatctcgagc cgaccggcgg caccgtgatg ctcgatccga acgagcgcct cggcaagctg
     3181 cgccaggacc agtttgcgtt cgaggaaatg cgcgtgctcg acgtcgtcat gatggggcac
     3241 accgagatgt gggccgcgat gaccgagcgc gacgcgatct acgcgaaccc cgaagcgacc
     3301 gatgacgact acatgcgcgc ggccgatctc gaagccaagt tcgccgagta cgacggctac
     3361 accgccgagg cgcgcgcggg cgaactgctg ctgggcgtgg gcgtgccgac cgagcagcac
     3421 aacggcccga tgagcaacgt tgcgcccggc tggaagctgc gcgtgctgct cgcgcaggcg
     3481 ctgttctcga atccggacat cctgctgctc gacgagccga ccaacaacct cgacatcaac
     3541 accatccgct ggctcgagaa cgtgctcaac gagcgcaact cgacgatgat catcatctcg
     3601 cacgaccgcc acttcctgaa ccaggtgtgc acgcacatgg cggacatgga ctacggcacg
     3661 ctgaagatct atccgggcaa ctacgacgac tacatgctgg cttccgcgca ggcacgcgag
     3721 cggcaggccg ccgccaatgc gaaggccaag gagcgcgtgg cggagctgca ggatttcgtg
     3781 cgtcgcttct cggccaacaa gtccaaggcg cgccaggcga cctcgcgcct gaagcagatc
     3841 gacaagatca agatcgacga cttcaagccg tcgtcgcgcc agaacccgtt catccgcttc
     3901 gagggcgaga agaagctgca ccgccaggcg gtggaagtgg agggcctgac caagtcctat
     3961 gaccgcacga tcttcaagaa catcaacctg atggttgagg cgggcgagaa gatcgccatc
     4021 gtcggcgcca acggcgccgg caagaccacg ctgctgcgct gcatcggtgc ggccgcgtgc
     4081 gccatggagc ccgacagcgg caaggtcaag tgggctgaac acgcgaacat cggctacatg
     4141 ccgcaggatc cgaccgagga gttcgcgacc gacatgaacc tgaccgactg gatgggccgc
     4201 tggacccagg agggcgacga cgaccaggcc gtgcgttcca tcctcggccg cctgctgttc
     4261 tccggcgacg atgtgaagaa gtcggtgaag gtgctgtcag gcggcgagaa gggccgcatg
     4321 atgtacggca agctgatgct gggccggcac aacgtgctgc tgctcgacga gccgaccaac
     4381 cacatggaca tggagtcgat cgaatcgctc aacatcgcgc tcgacaagta cgcgggcacg
     4441 ctgattttcg tatcgcacga ccgcgagttc gtgtcgtctc tggctagccg catcctcgag
     4501 gtgaaggaag gcgaagtgat cgatttccac ggcggctacg aggagtacct ggccagccag
     4561 gggatcgagt agccaggtcg ggccgcgcaa ggccgggccg cgctaggcgg gttgccggga
     4621 ccggcgcagc agcatctcga gctcgtgccg cagcgcacgc gcaacgtcgt tgccgcgcgc
     4681 ctgccacagg gcggccagcc acgcttcgcg cagagcgagc agctcgaccg acgatcgggc
     4741 cagctccacg cgcatctgca gcgtgtggcc gcgcagcccg aggttgcgcc ggatcgtgtc
     4801 cgtgtagaag cggtacagcg cctgatagag agggtcttcc atggtcgccg ggttgcgcgg
     4861 gttctggcgt attgccgtgc gcgtgacgct tgtcgtggcg gaagaatcgt tgccggccag
     4921 gcgcaccact gcagtttagg caagcgatta caatccgttc tatcggagcg caacagaacg
     4981 cgcgcgattg attgctgtca atcgggtaca gttttttcgc tgcctccttg aatctgccgt
     5041 cgcggcaccc agttacattg catttcacgc cgtcaagccg agtcgtttcc agaccatgca
     5101 aactctagaa atcaagcccg tcgaatttga actgccgcgc atggatgccg gcaccagctg
     5161 cacggcctcg gcctgggccc gcgtgccggc agcgccgtcg ccgcaggaaa aagccgcgct
     5221 gaaggaacgt atcaggcgcc tgctgaagga taggcaggcg gtcctggtcg cgcactacta
     5281 cgtcgatgcc gatctgcagg atctggccga ggaaaccggc ggctgcgtct ccgattcgct
     5341 ggagatggcg cgcttcggcc gtgaccaccc ggccaggacg ctggtggtgg cgggcgtgcg
     5401 cttcatgggc gagactgcga agatcctgag cccggagaag accgtgctga tgccggatct
     5461 ggacgcgacc tgttcgctgg acctgggctg cccggccgac gagttcgccg ccttctgcga
     5521 cgcccatccg gatcgcaccg tggtcgtgta cgcgaacacc agcgcggcgg tgaaggcgcg
     5581 cgcggactgg atggtgacgt cgtcgatcgg gctgcagatc gtcgagcacc tgcatgcgca
     5641 gggcaagaag atcctgtggg cgccggacaa gcatctgggc ggctacatcc agaagaagac
     5701 cggcgccgac atgctgctgt ggcagggctc ctgcatcgtg catgacgaat tcaaggggat
     5761 cgagctggaa ctgctgaaga aggagcatcc gggcgcaaag gtgctggtac atcccgaatc
     5821 tccggaagcg gtggtcgccc aggccgacgt ggtcggctcg acctcgcagc tgatcgcggc
     5881 ggcgcgcacg ctcgacgcgc aggaattcat cgtcgccacc gacaacggca tcctgcacaa
     5941 gatgaagatg gccgcgccgg gcaagacctt catcgatgcg ccgaccgccg gcaacagcgc
     6001 gacctgcaag agctgcgcgc actgcccgtg gatggcgatg aacggactgc agaacctggc
     6061 ggacgtgctc gagaccggac gcaacgaaat ccatgtcgat ccggagatcg ggcgcaaggc
     6121 ctttgcctgc atcgaccgca tgctcgactt cgcggcgaag cagaaggcca acgtacgtcc
     6181 gggcagcgac ctggcgaaag aacaacaact cttcaaggga atcgggcccg catgagcacg
     6241 ctcaagaatc cgttcgctcc cttcgatgcc gcactgaccg ccgcattcga agccaacatt
     6301 caggccgcgc tgaacgagga cgttggtagc ggcgacctga ccgggctcct ggtgccggag
     6361 gacgagcagg tcaaggcgcg ggtggttgtg cgggaggcgg ccgtgctgtg cggcgcgccg
     6421 tggttcgagg cggtgatgaa gcagctcgac gcgcgcatgc gcatccagtg gcattacgcg
     6481 gaaggcgaca tgatgcgtgc cgattccgag gtgtgcacga tcgacggccc ggcgcgcgcg
     6541 ctgctgaccg ccgagcgcac ggccttgaat ttcctgcaac tgctgtccgg cgtggccagc
     6601 gcgacccgcc gctatgtgga cgcgatcgcg ggcacccgct cggcgatcct cgatacccgc
     6661 aagacgctgc ctggcctgcg gctggcgcag aagtatgcgg tgcgcgtcgg cggcggccag
     6721 aaccagcggc tggcccttta cgacggcatc ctgatcaagg aaaaccacat tgcggcggcc
     6781 ggcggcatcg cgccggcgat gaaggcggcg ttcgcactcg acgccggcgt atcgatccag
     6841 atcgaggtgg aaagcctgga agagctggaa caggcgctcg ccgccggcgc gtcgtccata
     6901 ctgctcgaca acttctcgct cgacctgatg cgcgaggcgg tgcgcatcac cgcgggacgc
     6961 gcggtgctgg aagcctccgg cggcgtcaac atggaaacgg tgcgcgcgat cgccgagacc
     7021 ggcgtggacc ggatttcgat cggcagcctg accaaggacg tgcgcgccac cgattattca
     7081 ctgcgcatac tcgcctgact tgccaattcg acgacgcttt ttggcgtcgt ctctgctatc
     7141 aatggcggaa catctcgatg cattccgccg catgcccgct ctcgtcacct cggtcggcct
     7201 gttcaaaccg gacccggccg actcgttcac ctatcccgac cacatcgatc cggacagcct
     7261 ggcggcgctg ccgtcagcgc ccggcgtcta catctttctc gacgagcgcg agcgcccgct
     7321 ctacatcggc aagagcgtca acgtccgcag ccgggtgctg tcgcaccttc gcacgccgga
     7381 agaggcgcgc atgctgcagc agacgcggcg catcgacttt catcgcaccg ccggcgagat
     7441 cggcgcgctg ctgctggaat cgcggctcat caaggaccgg cagccggtgc acaacaagaa
     7501 gctgcggcgc gcgcgcgaga tgtgtgcgct acgcatcgca aacggcgtgc cggagatcgt
     7561 gcatgcacgc gaccatgact tcgcatccga gcacggcctg tacggcctgt tcgggacgcg
     7621 gcgggcggcg ctggagcggc tgcgccggtt ggtcgaggag cagcgcttat gcccggtgct
     7681 gtgtggagtg gagccggcgc ccaaggccgg gcgcgcctgc ttcgcctgcc aggtcggacg
     7741 ctgcgccggc gcgtgcgtcg ggcgcgaatc gcgcgacgcc cacgcgcggc ggctggccgg
     7801 cgcgctggag gacctgcgca tcgtgacctg gccctttccg ggaccggtcg gcatcatcga
     7861 gcgatgcgag ggctggcgcc agatgcacgt ggtcgatcgc tggttctacc ttggcaccgt
     7921 cgaccagccg cggcgcatgc gcgcgcctgc gcagaaaggg cggccgcggt tcgacgtgga
     7981 tacctattcg atcctggtgc ggccgttgct gctgggcgaa ctcgagcttc gcatgcttta
     8041 aggtgcgctg agcatgtcct cgcgcggcgc atcctgaatg caggcgcgcg acgtcgcagg
     8101 aatgattcag tgcaggctta agcttgacgc accgtcttca gcgcattgcc ccaggcaatc
     8161 agctggtcaa gcatcgtgcc gagcgtggct tcgtggcact gatccggctt gaacgtggtg
     8221 tagttctcga aatccgagga cagcgacagc atcacctggg cgcgcacgtc ggccacctgc
     8281 agctcgccca tcacgaggcg caggctctcg accgcgcgca cgccgccggc gctgccgtag
     8341 ccgacaaagc cggcggcctt gttgttccat tccttataca gaaaatcaat cgcgttcttc
     8401 aatgcgccgg aggtgccatg gttgtactcc ggtgtaacga acacgaaggc atcgaagctg
     8461 ccgatgcagt ccgcccaggc cttggtatgc ggcttgctgt actggtccat cgacggtggt
     8521 accggctcgt ccagcagcgg caggttgaaa tcctggatgt cgacgacttc gaactgtgca
     8581 tcgttgcgct ggcgggcgat ttcgtacgcc caattggcga cggcgtcagc cttgcggccg
     8641 gggcgggtgc tgcccacaat gatggctatc ctgatcatgg tcttgtcctt ctcgttgcaa
     8701 cggatgcagt gatagaacgg caagaccggt catggttcct ggggatgtgg atctgaaatc
     8761 gatggaaagg ggatgcatgc aacgcgttct gattgccgcc ggcgtgctgc tgctgatcat
     8821 aggcgtggcc tggccatggg tttcgcgcct gccgctcggc agattgccag gcgacatcca
     8881 catcgtccgg gagggcttca gcttctactt tccgatcgtc acctgcatcg tgatctcgat
     8941 cgtcgtgacg ctgcttttct ggatcttcag gcgttgagcg ggcgcgggag cgaggcgggc
     9001 cctgcgtccg tgcaggcggg caccggcgaa aaccccctca gcagaggttt tcgcccgggg
     9061 ttgccggctt gacgtgcgtt tcgcggacgg ctcgtaatgg ggctcagaat tcttcccagt
     9121 cgtcgccctt gcccggcggc ggggcagggc ggcgcgggat cggggcgacc gagcgttcgg
     9181 tggtgccggg cgcggcggcc ggcaccgccg gcttgaccgg tttggacggc acggcaacgg
     9241 cggcagcggg gcgcaccggc ttgggcgcca ccggggccaa cgtcacggcc tgattcgctt
     9301 cgatgcggaa cacgctgacc gcctgcgaca actggcgcgc ctggtcctgc atcgcttcgg
     9361 cggcggcggc ggcttcttcg accagcgacg cgttctgctg cgtggtctgg tccatctgcg
     9421 caatggcctg gttcacctgc tcgatgccgg aagtctgctc ggcactggcg gcggtgattt
     9481 cgcccatgat gtcggccacg cgcttgatgc tgtcgacgat ctcgtgcatc gtgctgccgg
     9541 cctcgttgac cagcctcgcg ccggtgtcga ccttctccac cgagttgtcg atcagcgcct
     9601 tgatttcctt ggcggcagcg gcggaacgct gcgcaaggct gcgcacttcc gacgccacca
     9661 c
//
LOCUS       DATLGF010000023         6387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_17383,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000023 DATLGF010000000
VERSION     DATLGF010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17383"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VM406_00730"
     CDS             complement(<1..335)
                     /locus_tag="VM406_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HVL74511.1"
                     /translation="MLSGLGLEWMAALLVAGVAIDLLLGEPRRWHPLVGFGNVAAWIE
                     RRLNRAPAGDAGPLAFRTLKTRLTGFAAWALCVIPFVALAAIAIEAAGPPASLVMHLL
                     LLYACLGLR"
     gene            complement(329..886)
                     /gene="cobU"
                     /locus_tag="VM406_00735"
     CDS             complement(329..886)
                     /gene="cobU"
                     /locus_tag="VM406_00735"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HVL74512.1"
                     /translation="MTRTLVLGGARSGKSVHAERLALAGGKQCIYLATALAGDAEMAA
                     RIAHHRARRDAGWITVEETLALGDALLRWCAADRVVVVDCLTLWLSNLLFSGAEALPE
                     HGVIDPPPAFGAQRDALLDALEQVRGDLILVSNEVGLGVVPAGAVSRWFVDEAGRLNQ
                     AVAARCDKVVFVAAGLPLVLKGDPC"
     gene            complement(883..2181)
                     /locus_tag="VM406_00740"
     CDS             complement(883..2181)
                     /locus_tag="VM406_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HVL74513.1"
                     /translation="MAASARTVLISAVSSGQGKTTVTAALARKFVRGGRRVRVFKTGP
                     DFLDPMMLERASGSPVHVLDLWMVGEERCRALLAQAATEADVILIEGVMGLYDGTPSS
                     ADLARAFGVPVLAVLDASAMAQTAGAVALGLRDYGPVRLAGVIANRIASERHAQMVAE
                     AMRDIPLFGTLLRQDASLPERHLGLVQPGEVAELDTLLDALADALVLDPGAWEALEPV
                     VFTAASAAAPADRPLNGRTVAIARDAAFAFLYPANIDCLRDLGATVVYFSPLADEGVP
                     AGSDAVYLPGGYPELHAARLAHADHWKSSMRETHVAGVPILAECGGMMALAEHLVDTG
                     GHRWPMAGLLNGTVVMQKRLAAIGGQGWDTAYGCLRGHAFHYSRFETDEAEYARTRRH
                     PAGGDGEAIYRKGSLTASYFHSYFPSCPQAVATLLGGGSP"
     gene            complement(2185..2793)
                     /gene="cobO"
                     /locus_tag="VM406_00745"
     CDS             complement(2185..2793)
                     /gene="cobO"
                     /locus_tag="VM406_00745"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HVL74514.1"
                     /translation="MSEDALNERHRARMQRKKALVDSKIDAATIHTGVLLVHTGNGKG
                     KSSSAFGMVMRALGHGMKVGVVQFIKGAKPTGEEMFLRRFPDEVSFHAMGEGYTWETQ
                     DRERDVAAAQRAWTKAGEFLNDPSIGLVVLDELNIALKYRYLDVERVVADLMSRPEMQ
                     HVVVTGRAAPPELIEAADTASEMQLLKHAFKAGIAAQPGVEW"
     gene            complement(2786..3565)
                     /locus_tag="VM406_00750"
     CDS             complement(2786..3565)
                     /locus_tag="VM406_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL74515.1"
                     /translation="MITVKKLDLSVGARTLVTGLDWQVRAGECWSVIGPNGAGKSTLL
                     RTVAGLRESDGGTVELLDKPLASWPIEELARQRALLPQGRSDAFAYSVIETVLTARHP
                     YHNAHYWESDDDYRIATSALQALEVDHLAERDVRTLSGGERQRVAIACVIAQQTPLVL
                     LDEPAAALDLAHQVAVMRLFHALAHREGKAVILISHDLNLAHAVSTHALLLMGDGRWE
                     AGPAQDVMQASLLGACLGHPIEKVMHGNRTLFIPAEDPVHV"
     gene            complement(3562..4617)
                     /locus_tag="VM406_00755"
     CDS             complement(3562..4617)
                     /locus_tag="VM406_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HVL74516.1"
                     /translation="MHIPTPISSVHHARPVDVRRRTLRIVGALAAASLAAVLFACSIG
                     SVPLTPGELLQAAGALLGFSDVSLNATLLELRLHRALVAFGTGASLALAGVMMQALLR
                     NPLADPYVLGISSGAAVGALGAMLFGALAWVVDAAALGGAVIVSALLFLLAQRDLRGG
                     LPSDSNATSLLLTGVILAAGCGALITLMLSIAPDNRLRNMVFWLIGDLSGAQLRWMPW
                     LVLGLAVLYALRSARGINLMAQHAEGAWTLGIRVSALRQGLFLSGAALAASAVTSAGS
                     IGFVGLIIPHACRFAFGPDHRLLLPAAALAGGGFLVLADTLARTIVAPQQLPVGVITA
                     LIGVPVFLMQLHHIRKK"
     gene            complement(4742..>6387)
                     /locus_tag="VM406_00760"
     CDS             complement(4742..>6387)
                     /locus_tag="VM406_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVL74517.1"
                     /translation="DLLQRQRGIEVTRTGGRGANASVFLRGADNRQNIVLVDGVRVGS
                     ATSGGANWSAIPLAQIERVEVVFGPMSSLYGADAMGGVIQIFTRRGEPGVRANASASA
                     GSHDTRILDAGISGASGRLRFAVAAAHEESDGFSSKKPGVLSGFNPDRDGYRKESANG
                     SVGFELAKGHEIGASFLRSRLYTEYDNTATLNDHQVQHVETYALTSSNRITSSWNSRV
                     QLARAYDKGSNYTTTVSRFNTRQNHLSWQNDIQFGKDDLVQLIVERREEEVDSTTTAL
                     NRTRSTNSVAAAYQLKRGAHLGSLIARNDNNSQFGSFATGSIGYGYWFTDALRANASL
                     GTSFRAPTFNELYFPSFGFAGNAPERGRNMEVGLRYDTGATRITASWYRNRIRDLLVT
                     ARPCPVPGFGTSCSYNVNEAILTGVSLGASTRLGNFVYRATVDLQDPKDETTGLRLAR
                     RARAHGTLGVEYDTNALGGPLVAGIEAVLSGKRFDDTSNLNRLPGYGLVNLHASYEFA
                     RNWTVFGRWNNVFDKDYELARGYATEGSAVFVGIRYAMR"
BASE COUNT         1113 a         2200 c         2100 g          974 t
ORIGIN      
        1 ctgcgcaggc cgaggcaggc gtatagcagg agcaggtgca tcacgagcga tgcgggtggt
       61 ccggcggctt cgattgcgat cgccgccagc gcaacgaacg ggatcacgca cagcgcccat
      121 gcggcaaatc ccgtcagccg ggttttcagc gtgcgaaacg caagcggtcc ggcatcgcca
      181 gcgggagccc ggttaagccg ccgctcgatc cacgccgcaa cattgccgaa gccgaccagc
      241 ggatgccagc ggcgcggctc gccgagcagc aagtcgatcg ccacgccggc aacgagcaaa
      301 gcggccatcc attcgagccc cagaccgctc agcacggatc gcccttcaga accagcggca
      361 agccggcggc gacgaacacc accttgtcac aacgcgccgc cacggcctgg ttcagacggc
      421 ctgcctcgtc gacgaaccag cgcgacaccg ccccggccgg caccacgccc aggccgactt
      481 cgttcgaaac caggatcagg tccccgcgca cctgctccag cgcgtccagc agcgcatcac
      541 gttgcgcacc gaacgccggc ggcggatcga tgacgccatg ctcgggcaag gcctcggcgc
      601 cggaaaacag caggttggac agccacagtg tcagacagtc gacgaccacc acgcggtccg
      661 ccgcgcacca ccgcagcagc gcatcgccga gcgcaagcgt ctcctcgacg gtgatccagc
      721 ccgcgtcgcg gcgcgcgcga tgatgcgcga tgcgcgcagc catttcggca tcgcccgcca
      781 gcgcggtggc gaggtagatg cattgcttgc cgccggccag cgccagtcgc tcggcatgca
      841 cgctcttgcc cgagcgggct ccgccgagca ccagcgtgcg ggtcatggcg atcccccgcc
      901 caacaaggtc gccaccgcct gcggacagga cgggaagtag ctgtggaaat acgatgcggt
      961 gaggctgccc ttgcggtaga tcgcctcgcc gtccccgccg gccggatgtc gccgcgtgcg
     1021 cgcgtactcg gcttcgtcgg tctcgaagcg cgaataatga aaggcatgcc cgcgcaggca
     1081 gccataggca gtatcccagc cctgcccgcc gatggccgca aggcgcttct gcatgacgac
     1141 cgtgccattg agcagcccgg ccatcggcca gcggtgtccg ccagtgtcga ccagatgttc
     1201 ggccagcgcc atcatccctc cgcattcggc aaggatgggc actcccgcca catgcgtctc
     1261 ccgcatcgac gacttccagt gatcagcatg ggccagtcga gctgcatgca gttcgggata
     1321 accgccgggc aggtaaaccg catcggatcc ggccggaacg ccctcgtcgg ccaacggcga
     1381 gaagtagacg acggttgcgc cgaggtcgcg caggcagtcg atgttggccg gataaaggaa
     1441 ggcgaacgcg gcatcgcgtg cgatcgcgac ggtgcggccg ttgagcggcc tgtcggccgg
     1501 cgcggcggcc gaagccgcag taaatacgac cggctcgagc gcctcccagg caccggggtc
     1561 caggacgagc gcatcggcga gcgcatcgag cagcgtatcg agttcagcca cttcgccggg
     1621 ctgcaccagg ccgaggtgac gttccggcag cgaggcatcc tggcgcaaca gcgtgccgaa
     1681 caacggaatg tcgcgcatcg cttctgccac catctgggcg tggcgttcgc tggcgatgcg
     1741 gttggcgatc acgccggcca gccgcaccgg cccgtagtcg cgcaggccga gcgcaaccgc
     1801 acccgcggtc tgcgccatcg cggaggcgtc cagcaccgcc agcaccggca cgccgaacgc
     1861 gcgcgcaagg tcggcggacg atggcgtgcc atcgtacaga cccatcacgc cctcgatcag
     1921 gatcacgtcg gcttcggtcg ccgcctgcgc cagcagcgcg cggcagcgtt cctcgccgac
     1981 catccacagg tcgagcacgt gcaccgggct gccgctggcg cgttcgagca tcatcggatc
     2041 gaggaaatcc gggccggtct tgaataccct cacgcgccgg ccgccacgca caaacttgcg
     2101 cgcaagcgcg gcggtgaccg tggtcttgcc ctggccggac gatactgcgg aaatcaggac
     2161 ggtacgggcg ctcgctgcca tcgtctacca ctccacgccg ggctgcgcgg caatgccggc
     2221 cttgaacgca tgcttgagca gctgcatctc gctggcagtg tcggcggcct cgatcagttc
     2281 cggcggcgct gcgcggccgg tgaccaccac gtgctgcatc tcgggccgcg acatcaggtc
     2341 cgccacgacg cgctccacgt cgagatagcg gtatttcagc gcgatattga gctcatccag
     2401 cacgaccagc ccgatcgacg ggtcgttcag gaattcgccc gccttcgtcc acgcccgctg
     2461 cgccgcggct acgtcgcgtt cgcggtcctg ggtctcccag gtatagccct cgcccattgc
     2521 gtgaaaggac acttcatcgg gaaagcggcg caggaacatc tcctcgccgg tcggctttgc
     2581 gcccttgatg aattgcacga caccaacctt catcccgtgg ccaagcgcgc gcatgaccat
     2641 gccgaacgca ctcgaactct tgcccttgcc attgccggta tgaaccagca gcacgccggt
     2701 gtgaattgtg gcggcgtcga tcttgctgtc caccagcgcc ttcttgcgtt gcatgcgcgc
     2761 gcggtggcgt tcgttcaggg cgtcttcaga catggacagg atcctcagcg ggtatgaaaa
     2821 gggtacggtt gccgtgcatg actttctcga tcggatgacc gaggcaggcg ccgagcagcg
     2881 atgcctgcat cacgtcttgt gcagggcccg cctcccagcg gccgtcgccc atcagcagca
     2941 gcgcgtgcgt ggagaccgcg tgggcaaggt tcaggtcgtg gctgatcagg atcacggcct
     3001 tgccctcgcg atgcgcgaga gcatggaaca ggcgcatcac ggcgacctgg tgtgcgagat
     3061 cgagcgcggc cgccggctcg tccagcagga ccagcggcgt ctgctgcgca atcacgcagg
     3121 caatcgccac gcgctggcgt tccccgcccg acagtgtgcg cacatcgcgc tcggcaagat
     3181 ggtcgacttc cagcgcctgc agcgcggatg tggcgatcct gtagtcgtcg tcggattccc
     3241 agtaatgcgc attgtggtac gggtggcgcg cggtcagcac cgtttcgatc acgctgtagg
     3301 cgaaggcgtc gcttcgtccc tgcggcaaca gcgcccgctg gcgcgccagt tcctcgatcg
     3361 gccaggacgc gagcggcttg tccagcagct caaccgtgcc gccgtcggac tcgcgcaggc
     3421 cggcaacggt acgcagcagc gtgctcttgc ccgcaccgtt cgggccgatc acactccagc
     3481 actcgccggc ccggacctgc cagtcgagac cggtgacgag ggtgcgcgcg ccgacggaga
     3541 gatcaagttt ttttacggtg atcatttctt gcggatgtgg tgcaactgca tcaggaatac
     3601 cggcacgccg atcagcgcgg tgatcacacc caccggcaac tgctggggcg cgacgatcgt
     3661 gcgcgcgagc gtatctgcca ggaccaggaa accgcccccc gccagcgcgg cggccggcaa
     3721 cagcaaccga tgatcggggc cgaacgcaaa ccggcacgcg tgcggaatga tcaggccgac
     3781 gaagccgatg ctgccggcgc tggtgacggc gctcgcggca agcgcggcgc cggaaagaaa
     3841 caagccctgc cgcaaagccg acaccctgat gcccagcgtc catgctcctt cggcgtgctg
     3901 cgccatcagg ttgatgcccc gcgccgaacg cagcgcatac agcacggcga gaccaagtac
     3961 gagccatggc atccagcgca actgcgcgcc ggacaagtcg ccgatcagcc agaacaccat
     4021 gttgcgcagg cggttgtccg gcgcgatcga cagcatcagc gtgatcagcg cgccgcagcc
     4081 tgcggcgagg atcacgccag tcagtaacag cgacgtggcg ttgctgtcgc taggcaggcc
     4141 gccgcgcaga tcgcgctggg caagcaggaa caacagcgcc gacacgatca ccgctccacc
     4201 cagtgcggcg gcatcgacca cccaggccag cgcgccgaac agcatcgcac caagcgcgcc
     4261 gaccgccgcc ccgctggaaa tgcccagcac atagggatcg gcgagcgggt tgcgcagcag
     4321 cgcctgcatc atcacgccgg ccagcgcgag cgaggcgcca gtaccaaacg cgaccaaggc
     4381 gcggtgcagg cgcagttcga gcagcgtcgc attcaggctc acgtcggaaa agcccagcaa
     4441 tgcgccagcc gcctgcagca gctcacccgg ggtcagcggc accgaaccga tcgagcaggc
     4501 aaacagcacc gccgcaagcg aagccgcagc gagcgcgccg acgatgcgca gcgtgcggcg
     4561 gcgcacatcg accggacgcg catggtgaac ggagctgatg ggagtgggga tgtgcattgg
     4621 ttgtaagaat ccttgcgtcg acgcgtgcgc cgcgcggcta tcgcagccct cgtccccttg
     4681 cagccttact gccttgcggc cgccagcgga aagggcttcg gcaccacgac tgccgcggcc
     4741 ctcagcgcat cgcatacctg atgccgacga acaccgccga cccttcggtc gcatagccgc
     4801 gcgcgagttc gtaatccttg tcgaacacgt tgttccagcg gccgaacacg gtccagttgc
     4861 gcgcgaactc gtagctcgcg tgcaggttca ccagcccgta gcccggcagg cggttcaggt
     4921 tgctggtgtc atcgaatcgc ttgcccgaca gcacggcctc gatcccggcg accagcggtc
     4981 ctccgagggc gttcgtatcg tactccacac ccagcgtacc gtgggcgcgc gcgcgtcgtg
     5041 ccaggcgcag accggtggtt tcgtccttcg ggtcctgcag gtcgacggtg gcccggtaca
     5101 cgaagttgcc gaggcgcgtg ctggcgccga gcgatacgcc ggtcagaatc gcctcgttga
     5161 cgttgtacga gcaactggtg ccgaagcccg gcaccgggca tggccgcgcg gtgaccagca
     5221 gatcgcggat cctgttgcgg taccaggacg cggtgatgcg cgtggcgccg gtgtcgtagc
     5281 gcaatcccac ctccatgttg cgcccgcgct ccggcgcgtt gcccgcaaac ccgaagctgg
     5341 ggaagtacag ctcattgaag gtcggcgcgc ggaagctggt gccgagcgag gcattggcac
     5401 gcagcgcatc ggtgaaccag tagccgtagc cgatcgaacc ggtcgcgaac gagccgaact
     5461 gcgaattgtt gtcgttgcgc gcgatcaggc tgccgaggtg ggcgccgcgc ttcaactggt
     5521 aggcggccgc gacggaattg gtgctgcgcg tgcggttcag cgcggtggtg gtcgaatcga
     5581 cctcttcctc gcgccgctcc acgatcagct gcaccaggtc gtccttgccg aactggatgt
     5641 cgttctgcca ggacagatgg ttctggcgtg tattgaagcg cgacacggtg gtcgtgtagt
     5701 tgctgccctt gtcgtacgcg cgcgccagct gcacgcggct gttccagctt gaagtgatgc
     5761 ggttggacga agtcagcgca taggtctcga cgtgctgcac ctggtggtcg ttcagcgtgg
     5821 ctgtgttgtc gtactcggta tagagccggc tgcgcaagaa ggatgcgccg atctcgtgcc
     5881 ctttggcgag ctcgaaaccg acgctgccgt tggcactttc cttcctgtag ccgtcgcgat
     5941 ccgggttgaa tccagacagc acgccgggct tcttcgacga gaatccgtcc gactcctcgt
     6001 gagccgctgc caccgcgaaa cgcaggcgcc cggaggcgcc cgatatgcct gcatcaagga
     6061 tgcgcgtgtc gtggctgcct gcgctggccg acgcgttcgc gcgcacgcca ggctcgccgc
     6121 gcctggtgaa gatctggatc acgccgccca ttgcgtcggc accgtacagg ctgctcatcg
     6181 gcccgaacac gacttcgacg cgttcgatct gggccagcgg aatggcggac cagttcgccc
     6241 cgccggaggt ggccgaaccc acgcgcacgc catcgaccag cacgatgttc tggcgattgt
     6301 cggcgccgcg caggaagacc gaggcgttcg cgccgcggcc gcccgtgcgc gtgacctcga
     6361 tgccgcgctg gcgctgcagc agatcga
//
LOCUS       DATLGF010000024         2986 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_80540,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000024 DATLGF010000000
VERSION     DATLGF010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80540"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VM406_00765"
     CDS             complement(<1..792)
                     /locus_tag="VM406_00765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HVL74518.1"
                     /translation="MFQAILLNKNEDGTTDARLTQLDDDQLPQDGDVTVEVAYSTINF
                     KDALAITGKSPVVRKWPMVPGIDGAGRVVESTHPDWKSGDGFILNGWGVGEVHMGCLA
                     QRARLKGDWLIPLPAGMNPRTAMAIGTAGYTAMLCAMALREQGVEPGSGEILVTGASG
                     GVGSVAVAILAKRGYRVVASTGKLNEAEYLTSLGAAEVIDRAQLSAPGKPLQKERWAG
                     VVDAVGSHTLANAIAQTRYGGAVAACGLAQGMDLPASVAPFILRGV"
     gene            complement(911..1633)
                     /locus_tag="VM406_00770"
     CDS             complement(911..1633)
                     /locus_tag="VM406_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HVL74519.1"
                     /translation="MKTLIRLTSAALLALCATAAFAQSGIEASIKKTLEPRLGEGVKV
                     DSITRTPYSGLYEVRIGNDIFYTDAKGEYLFLGRIVDTKNFQDHTKARLDEINRIKFD
                     ELPFESALKMVRGNGKRVIAVFEDPNCGYCKRFRQTLNQMDNVTVYTFMYNILSEDSA
                     VKSRNVWCSADRNKAWDDWMLNGKVPASAPANCTAPNEAIRALGQRLNVTGTPTIFFT
                     DGSRIPGAIDAKGLESKLTSIK"
     gene            complement(1666..2856)
                     /locus_tag="VM406_00775"
     CDS             complement(1666..2856)
                     /locus_tag="VM406_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF family hydroxylase"
                     /protein_id="HVL74520.1"
                     /translation="MQLACDILVIGDGAVGKTAALGLAQAGLDVIVLRPGAARPATSP
                     EEWDVRVYALNRVAHDLLAALKVWPALDTSRIAEVDSMIVYGDGPAAGNLVFDAYGAR
                     STELAWIVEDRNLNVALDAALRFSPNLRSVSGNATRLEVDASHAAVTLDNGDVLRATL
                     VVGADGANSWVRGQCDIDLDYRSYGQRAIVSNFACEHPHHGAAYQWFSASEGIIALLP
                     LAGQRVSLVWSAPEALATSLLEGSLTQLARRLCALPGHRLGRLTPLQPEAVKSFPLSL
                     QRPHQIVAPRVALIGDAAHVVHPLAGHGMNLGFADVNELIRRIGEREAHRDCGDERVL
                     ARYARARKEDIFLMQAATDGLERLFSTDFEPVRIARNLGLNLVNKFPVLKRALISHAL
                     GRRS"
BASE COUNT          521 a          946 c          979 g          540 t
ORIGIN      
        1 gacgccgcgc agaataaagg gtgccaccga ggccggcaga tccatgccct gggcgaggcc
       61 gcatgcggca acagcgccgc cgtagcgggt ctgggcgatc gcattggcaa gcgtgtgcga
      121 gccgaccgca tccaccacgc cggcccagcg ttccttctgc agcggtttgc ccggggccga
      181 cagttgcgcg cgatcgatca cctcggccgc gcccagcgat gtcaggtact cggcttcatt
      241 gagcttgccg gtcgacgcga cgacccgata accgcgcttc gcgagtatcg ccaccgcgac
      301 gctgccgact ccaccggagg cgccggtgac gagaatctcg ccgctgccgg gttcgacgcc
      361 ctgctcgcgc aaggccattg cgcacagcat tgcggtatag cccgcggtcc cgatggccat
      421 cgccgtgcgc ggattcatgc cggccggcag cggaatgagc cagtctccct tgagccgtgc
      481 gcgctgcgcg aggcaaccca tgtgcacttc gccgacgccc cagccgttga gaatgaagcc
      541 atcgccgctc ttccagtccg gatgcgtcga ttcgacgacc ctgccggcac catcgatgcc
      601 gggcaccatc ggccatttgc gcaccaccgg cgacttgccg gtaatggcca gcgcatcctt
      661 gaaattgatc gtcgagtacg cgacctcgac cgtgacgtcg ccgtcctgtg gcagctggtc
      721 atcgtcgagt tgcgtgagcc gggcgtcggt ggtgccgtct tcgttcttgt tgagcagaat
      781 cgcctggaac atgtcgtctc cttccggggg gagaaacgat tatagagcgc gatgccgaat
      841 gacgcggccg acccgacagg cgaaaaaaat gccgcaggcg atgcggcatt tcttgacgat
      901 gcggtctgcc ttacttgatg gaggtgagct tgctctccag gcccttcgcg tcgatcgcac
      961 ccggaatgcg cgagccgtcg gtgaagaaga tggtcggcgt gccggtcacg ttcaggcgct
     1021 ggccgagcgc gcggatcgct tcattcggtg cggtgcagtt ggccggcgcg ctggccggga
     1081 ccttgccgtt tagcatccag tcatcccagg ccttgttgcg gtcggccgag caccagacat
     1141 tgcgcgactt gaccgccgaa tcctcggaca ggatgttgta catgaaggtg tagacggtca
     1201 cgttgtccat ctggttgagc gtctggcgga agcgcttgca gtagccgcag ttcggatcct
     1261 cgaacacggc gatcacacgc ttgccgtttc cgcgcaccat cttcagtgcc gattcgaacg
     1321 ggagctcgtc gaacttgatc cggttgatct catcgaggcg cgccttggtg tgatcctgga
     1381 agttcttcgt gtcgacgatg cggcccagga ataggtattc gcccttggcg tcggtgtaaa
     1441 aaatgtcgtt gccgatgcgc acttcgtaca agccgctgta tggggttctc gtgatcgagt
     1501 cgaccttcac accctcgccg agccgcggct ccaacgtctt cttgatcgag gcttcgatgc
     1561 ccgactgggc aaatgcggcg gtggcacaga gcgccagcag cgcggcagag gtcaggcgga
     1621 tgagtgtctt cattgtgtgt ccggttgcgc cacgaatggc ggtgttcagc tgcgccgtcc
     1681 cagagcgtgg gagatcagcg cacgctttaa gacaggaaac ttgttaacca agttcaatcc
     1741 caggttgcgg gcgatccgaa ccggttcgaa gtcggtggag aacaggcgct ccagtccatc
     1801 ggtggccgcc tgcatcagga aaatgtcttc cttgcgcgcg cgcgcatagc gcgccagcac
     1861 gcgctcgtcg ccgcaatcgc gatgcgcttc gcgctcgccg atgcggcgga tcagttcgtt
     1921 gacatctgca aaaccgaggt tcatgccatg gccggcgagc ggatgcacga catgcgcggc
     1981 atcgccgatc agggccaccc gcggggcgac gatctgatgc ggccgctgca gcgacagcgg
     2041 gaaggacttg accgcctccg gctgcagcgg cgtcaggcgt ccgagccggt ggccaggcaa
     2101 cgcgcacagg cggcgggcaa gctgggtgag cgaaccctcc agcaggctgg tggccagtgc
     2161 ttccggcgcc gaccacacca gcgaaacgcg ctgaccggcc agcggcaaca gcgcgatgat
     2221 gccctcgctg gcggagaacc attgataggc tgcgccgtga tgcggatgct cgcaggcgaa
     2281 attgctgacg atcgcacgct ggccgtagct gcggtaatcg agatcgatgt cgcactgtcc
     2341 gcgcacccac gagttcgcgc cgtctgcgcc gacgaccagc gtggcgcgca gcacatcgcc
     2401 gttgtcgagg gtgacagccg cgtggcttgc gtcgacctcg agccgggtcg cattgccgct
     2461 gacgctgcgc aagttcggcg agaagcgcag cgcggcgtcg agtgccacgt tcaaattgcg
     2521 gtcctcgacg atccacgcga gttcggtgct gcgcgcgcca taggcgtcga acaccaggtt
     2581 gcccgcggcg ggcccgtcgc cgtacacgat catcgaatcg acttcggcaa tgcgcgatgt
     2641 gtcgagcgca ggccagactt tcagcgcggc cagcaggtca tgagcaacgc gattgagcgc
     2701 atacacgcgc acatcccatt cctccgggga ggtggctggg cgggcggcgc ccggccgcag
     2761 gacgatcacg tcgaggccgg cctgggccag ccccaacgcg gcggtcttgc cgaccgcgcc
     2821 gtcaccgatg accagaatgt cgcaagcgag ctgcataagt ggcaatgcaa gaaaaaaccg
     2881 gtccattata gctgctgcga ttttcgctgc cgggcggaaa tgcttgcaca atctcacggc
     2941 tttgctatac ttgcgcgtct tggcccggta gctcagttgg tagagc
//
LOCUS       DATLGF010000025         6732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_159226, whole genome shotgun sequence.
ACCESSION   DATLGF010000025 DATLGF010000000
VERSION     DATLGF010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6732
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159226"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..2075
                     /locus_tag="VM406_00780"
     CDS             96..2075
                     /locus_tag="VM406_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HVL74521.1"
                     /translation="MNTILIANRGEIALRIMRTARRLGFRCAAVYSDADAGAEHVRAA
                     DAACGIGAGPARDSYLNVPAILAAARKLGADAVHPGYGFLAENAEFAEAVRDAGLVWI
                     GPSPAAMRAMGNKAAAKRLLAERDVPMLPGYQDSQQDAATLLREASRIGLPLMIKAAA
                     GGGGRGMRLVREEQELAEALARARSESEQAFGSGELILERALIAPRHVEVQVFADSRG
                     NVVHLGERDCSVQRRHQKLIEEAPSPAVDAALRARLGAAAVQAARACGYVGAGTIEFL
                     LDADGGFWFMEMNTRLQVEHPVTEEITGLDLVEWQLRIAQGEALPLSQEDIDARLARG
                     GHAIEVRLCAEDPAAAFLPQSGRVLSWMAAPDLRVEHALSDGAEVPPYYDSMLAKIVA
                     RGANRDEARRRLLRGLQTTRLLGIASNQDFLADCLAHPGFAAGEARTDFIDAHFPASA
                     RAAPAPSFDVQCAAAVVALARRLPAAPAYPDELAGWNSSGAFADSLDLEIDDTRCSVP
                     VRANGPLAWIIGTEGQAVRVRIAERDAAWVRLEIDGGVQETVGLAASGDLVYWRYRNR
                     NHTLRDLIREPAQTGASRQGGGLIRAPMSGKVTAVHVTAGASVKAGEAMLVLEAMKME
                     HTVTAPCAGVVEALHVHAGDQAGPGMVLAEIRPQA"
     gene            2095..3264
                     /locus_tag="VM406_00785"
     CDS             2095..3264
                     /locus_tag="VM406_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVL74522.1"
                     /translation="MLKPNPAWYTPTHEDFRAAVRRFVANEIAPHAAEWDEDETFPRE
                     LYEKAAAIGLLGLGFPERFGGTPDADPFHHLIASEELARCGSGGIVASLMSHSIGAPP
                     ILFAGRPELQERVLPQIIAGTKIAALAVTEPGGGSDVAALRTSARREGDHYIVNGEKT
                     FITSGMRADYYTVAVRTEPEAQGAAGISLLLIERDAPGFSRTPLKKMGWWASDTAHLR
                     FEGCRVPAANLLGEEGKGFRLIMRNFNMERFNLAAGAWGFAQVCHEDALAWAQERRTF
                     GKRLVEHQVVAHKLVDMATQLAATRAMLEDTAWRLAGPHQQGGELVAQLCMLKNLATR
                     TMQFCADAAVQTLGGMGYMRGTRVERIYRDVKVNMIGGGAEEVMKDLAARQLGYT"
     gene            3294..3794
                     /locus_tag="VM406_00790"
     CDS             3294..3794
                     /locus_tag="VM406_00790"
                     /inference="COORDINATES: protein motif:HMM:NF008675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HVL74523.1"
                     /translation="MKDTNLRMIDTPDFQNALRRILEEMIPFCRVLGIRLASFDPENP
                     QLEFAMRDELLGNVGKGMLHGGAIAAVLDTMAGYAIFLKMTQEDPKPDLMAQLQEFAR
                     MSTIDLRVDYLQPGRGERFTASATVTRLGRRVANVQMALRNEEGVCIATGAAAFMLHA
                     PKSGAG"
     gene            3938..5518
                     /locus_tag="VM406_00795"
     CDS             3938..5518
                     /locus_tag="VM406_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HVL74524.1"
                     /translation="MLRNLSIRVRLIGVLAFLALQITLGAIVGIYSLSMSNSDMQDVY
                     WNRLVPTGQLDRVVRLLQENQLTLAKAVANHQLLAKAISGDAAEYQAQLEQIENNSRE
                     IERQWTSYTASALTPQERSLAAKFDAARASFQTEALTPAVQALKEYNTVVASQILYGR
                     MAELFAPLDETIDALIQLQLDTARIQYERSQSAYLLIRNICVAAVLIGLLLAAFVGTW
                     LVRAICGPLDQAAALAGSVAQGDLSRDIRIASEDETGRLLQALSNMTTSLSRIVAQVR
                     QGADAMSTASAEIAAGNQDLSGRTEQQASALEETAASMEELTATVKQNASNAEQANQL
                     AQAASDVAGRGGAVVQQVVGTMDDIHASSKKIVDIIGVIDGIAFQTNILALNAAVEAA
                     RAGEQGRGFAVVASEVRNLAQRSAAAAKEIKALIDSSVGKVESGARLVNEAGSTMDEI
                     VSSIHKVAAMMSEITTASAEQTSGIEQVNRAIAQMDQVTQQNAALVEQAAAAAAAMQQ
                     QASSLAQAVSVFHIRSGR"
     gene            complement(5879..>6732)
                     /locus_tag="VM406_00800"
     CDS             complement(5879..>6732)
                     /locus_tag="VM406_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL74525.1"
                     /translation="TIGTPVVMATLPILFERNVPNMFPLTAAREMYEPFHRLKFSFAA
                     PYYNQARSGVQFMAKERGAKSFCIVYQDDEFGLEVMRGAEEGLQKLGLNWTERTTYKR
                     GATDFSSQVARMRASGCDTVVMGTIIRETIGVIGEARKSGWNPNFIGTSASYTELIHR
                     LGGKAMDGFYSAFQIGVPYLDDASKNVRDWGARYKDRFKEDPTVFSAYGYSMITMFAA
                     AAEKAGPNLTTDTLISTMESMWFPRDMFGSPGYRFTSTNRLGNDRSRIGQIQNGKWVA
                     VTDYLDY"
BASE COUNT         1223 a         2142 c         2271 g         1096 t
ORIGIN      
        1 gcgcaggccc tgcgcaacgg cgacgccgcc gaaggggtgc gcgccttcac cgagaaacgc
       61 ccgccgtcct gggcacagca agcggaacag cagacatgaa caccatcctg atcgcgaacc
      121 gcggagagat cgcgttgcgc atcatgcgca ccgcgcgccg gctcgggttt cgctgcgcgg
      181 ccgtgtactc cgacgcggat gccggcgccg agcacgtccg cgcggccgac gcggcctgcg
      241 gcataggtgc cggtcccgcc cgtgattcct acctgaacgt acccgccatc ctcgcggctg
      301 cgcgaaagtt gggtgccgat gcggttcacc ccggctacgg ctttttggcg gagaacgcgg
      361 aattcgccga agccgtgcgc gacgccggac tcgtctggat cggcccgtcc cctgccgcga
      421 tgcgcgcaat gggcaacaag gccgccgcca agcgcctgct ggccgagcgc gacgtgccga
      481 tgctgccggg ctaccaggac agccagcagg acgcggcgac gctgctgcgc gaagcgtcgc
      541 gcatcggcct gccgctgatg atcaaggccg ccgccggcgg cggcgggcgc gggatgcggc
      601 tggtgcggga ggaacaggag ctcgcggaag cactggcgcg tgcgcgttcg gagtcggagc
      661 aggcattcgg cagcggcgag ctgattctcg aacgcgcgct gattgcgccg cgtcacgtcg
      721 aggtgcaggt gttcgcggac agccgcggca atgtcgtgca cctgggcgag cgcgactgct
      781 ctgtgcagcg ccgtcaccag aaactgatcg aggaagcgcc gtcgccggcc gtcgatgccg
      841 cgctgcgtgc gcgcctgggc gctgccgcgg tgcaggccgc gcgcgcctgc ggctatgtag
      901 gcgccggcac gatcgaattc ctgctggatg ccgatggcgg cttctggttc atggagatga
      961 atacgcgcct gcaggtcgag catccggtga ccgaggaaat caccgggctg gacctggtcg
     1021 agtggcagct gcgtatcgcg cagggcgaag ccttgccgct gtcccaggaa gacatcgatg
     1081 cgcgccttgc gcgcggcgga cacgcgatcg aggtacgcct gtgcgccgaa gatccggcgg
     1141 cagcgttcct gccgcagagc ggacgcgtgc tgtcttggat ggcggccccg gaccttcgcg
     1201 tcgaacatgc actgtccgat ggggccgagg tgccgcctta ctacgattcg atgctggcga
     1261 agatcgtcgc ccgtggcgca aaccgcgacg aagcgcggcg ccggctgctg cgcgggctgc
     1321 aaacgaccag gctgctcggt atcgccagca atcaggactt tctcgccgat tgcctcgccc
     1381 atccggggtt cgcggcgggc gaggcacgca ccgatttcat cgatgcgcac tttccggctt
     1441 ccgcgcgggc cgcgcctgcg ccgtcgttcg acgtgcaatg tgcggcagcg gtcgttgcac
     1501 tggcacggcg gctgcccgcg gcgccggcct atccggacga actggccggc tggaacagca
     1561 gcggcgcatt tgccgacagc ctcgatctcg agatcgacga cacacgctgt tcggtgccgg
     1621 tccgtgccaa cggtccgttg gcatggatca tcggcacgga gggacaggcg gttcgtgtac
     1681 gcatcgccga gcgcgatgcg gcttgggttc gcttggagat cgacggcgga gtccaggaga
     1741 cggtcggtct tgcggcctcg ggcgatctgg tctactggcg ctaccgtaat cgcaaccaca
     1801 cgctgcgcga cctcatccgc gagccggcgc aaaccggcgc aagccgccag ggtggaggtc
     1861 tgatccgcgc gccgatgagc ggcaaggtca ccgccgtgca tgtgacggct ggcgcgagcg
     1921 tcaaggcggg ggaggcaatg ctggtgctgg aggcgatgaa gatggagcat acggttaccg
     1981 cgccctgcgc cggtgtcgtg gaggcgcttc acgttcatgc gggcgaccag gccggacccg
     2041 gcatggtgct ggccgagata aggccgcagg cctgaccgca gcgaggaccg caacatgctc
     2101 aaaccgaacc ctgcctggta cacgccgacc catgaggact tccgcgccgc cgtgcgccgc
     2161 ttcgtcgcaa acgaaatcgc tccgcacgcc gccgagtggg acgaggatga gaccttcccg
     2221 cgtgagctgt acgaaaaggc ggccgcaatc ggcctgctcg gcctgggttt tcccgagcgc
     2281 ttcggcggca cgccggacgc cgaccccttt catcatctaa tcgcctccga agagctggcg
     2341 cgctgcggca gcgggggcat cgtcgcttcg ctgatgtcgc acagcatcgg cgcaccgccg
     2401 attctgttcg ccggccgccc cgagctgcag gagcgggtgc tgccgcagat catcgccggc
     2461 acgaagatcg ccgcgctggc agtcacggag ccgggcggcg gttcggacgt ggccgcgctc
     2521 aggacaagcg cgcggcgcga aggcgatcac tacatcgtca atggcgaaaa gaccttcatc
     2581 acctcgggca tgcgcgccga ctactacacg gtcgccgtgc gcaccgaacc cgaggcgcag
     2641 ggcgcggcag gcatctcgct gctgctgatc gagcgcgacg cacccggttt ttcgcgcacg
     2701 ccgctgaaaa aaatgggctg gtgggcgtcc gacaccgcgc atctccggtt tgaaggctgc
     2761 agggtgccgg ctgcaaacct gctgggcgag gaaggcaagg gcttccggct catcatgcgc
     2821 aacttcaaca tggagcgctt caatctcgcc gccggcgcct ggggctttgc ccaggtctgc
     2881 catgaagatg cgctcgcctg ggcgcaggag cgtcgcacgt ttggaaaacg gctggtcgaa
     2941 caccaggtgg tcgcgcacaa gctggtggac atggccaccc agctggcggc cacgcgcgcg
     3001 atgctcgagg ataccgcatg gcggcttgcc ggcccgcatc agcagggcgg cgagctggtc
     3061 gcgcagctgt gcatgctgaa gaacctcgcc acccggacca tgcagttctg cgccgacgcg
     3121 gcggtccaga cgctgggcgg catgggctat atgcgcggca cccgcgtcga acggatctac
     3181 cgcgacgtca aggtcaacat gataggcggc ggcgccgagg aagtgatgaa ggacctggcc
     3241 gctcgacagc tcggatacac atgaatgcgt ggtgcggcca cgaacagcaa gcgatgaaag
     3301 acacgaacct gcgcatgatc gacaccccgg atttccagaa cgcgctgcgg cgcatcctgg
     3361 aagagatgat cccgttctgc cgcgtgctcg gcatccggct tgcgtcgttc gatccggaaa
     3421 atccccagct cgaattcgcg atgcgcgacg aactgctcgg caacgtcggc aagggcatgc
     3481 tgcatggcgg tgcgatcgcg gcagtgctcg acacgatggc cggctatgcg atcttcctga
     3541 aaatgaccca ggaggatccg aaaccggacc tgatggccca gttgcaggag tttgcgcgca
     3601 tgagcacgat cgacctgcgc gtcgactacc tgcagcccgg aaggggtgaa cggttcaccg
     3661 cgtccgccac cgtgacgcgg ctgggccggc gcgtggccaa cgtgcagatg gccttgcgca
     3721 acgaggaggg cgtctgcatc gcgaccggcg cggccgcgtt catgctgcat gcgccgaaga
     3781 gcggagcagg ctagcgtact gcgcaagccg cgatctcggt ccgaacctgt tacatgtgcg
     3841 caacattaaa tatgaacaat acgcactaaa ttcacgcgga gcttggttgc tcactctcag
     3901 cgaaacagaa tcattgtatt cactggagga gacaaccatg ctgcgtaacc tgagcatccg
     3961 tgttcgcctg atcggcgtgc ttgcatttct ggcactgcag atcacgcttg gcgcaatcgt
     4021 gggcatctac agcctgtcga tgtcgaacag cgacatgcag gatgtctatt ggaaccggtt
     4081 ggtcccgacc ggccagctcg atcgcgtggt acgcctgctg caggaaaacc agctgacctt
     4141 ggccaaggcg gttgcgaatc accagttgct tgccaaggcg atcagcgggg acgccgccga
     4201 gtaccaggcc cagctcgaac agatcgagaa caactcccgc gagatcgaac ggcagtggac
     4261 gtcctacacg gcaagcgcgc tgacgccgca ggagcgcagc cttgccgcga aattcgacgc
     4321 cgcccgcgcc agcttccaga ccgaagcgtt gactcccgca gtacaggcgc tcaaggagta
     4381 caacaccgtc gtcgcgagcc agatcctgta tggccgcatg gcagaactgt tcgcgcctct
     4441 cgacgagacg atcgatgcgt tgatccagct tcagctcgac acagccagga tccaatacga
     4501 gcgttcgcaa tcggcctacc tcctgatccg caacatctgt gtggcggccg tcctgatcgg
     4561 cctgctgctc gcggcctttg tcggcacctg gctggtgcgc gcgatttgcg gcccgctcga
     4621 tcaggccgcc gcgctggccg gcagcgtggc gcagggcgat ctctcgcggg acatccggat
     4681 tgcgagcgag gacgagacgg gtcggctgtt gcaagcactg tcgaacatga cgaccagcct
     4741 ttcgcgcatc gtggcccagg tccggcaggg cgctgacgcg atgtcgaccg cgtcggccga
     4801 aatcgccgcc ggcaaccagg acctgtccgg gcgcaccgag cagcaggcca gcgcgctgga
     4861 agagacggct gcgtcgatgg aggaactgac tgcgaccgtc aagcagaacg cgagcaatgc
     4921 cgagcaggcc aatcagcttg cgcaggcagc ttcggatgtc gccggccgtg gcggcgcggt
     4981 ggtgcagcaa gtggtgggca cgatggacga catccatgcc tcctcgaaga agatcgtcga
     5041 catcattggc gtgatcgacg gcatcgcctt ccagaccaac atcctcgcat tgaatgcggc
     5101 ggtggaggcg gcgcgtgcgg gcgagcaggg gcgcggcttt gccgtggtcg cgtccgaagt
     5161 gcgcaacctc gcgcagcgtt ctgccgcggc cgccaaggag atcaaggcct tgatcgattc
     5221 ctccgttggc aaagtcgaat cgggcgccag gcttgtgaac gaggccggct caacgatgga
     5281 cgagatcgtg tccagcattc acaaggtggc ggcgatgatg agcgagatca cgacggccag
     5341 cgcggagcag acgtccggca tcgagcaggt caatcgcgca atcgcccaaa tggaccaggt
     5401 cacgcaacag aacgccgcgc tggtcgagca agctgcggcc gcggcggcag ccatgcagca
     5461 acaggcatcc agcctcgcgc aggcggtcag cgtatttcac atccgcagcg gaaggtgacc
     5521 cccggtgtgc ggcgatcagg tccctcgctg cgctcgggac gacaggtggt tgggatattc
     5581 gccgtgcatc cgccgtgttc gccgcggtga aagacaacgg ttcaaaccct gaaccacggc
     5641 gggcacggca accacggcga aaaagcttgt cgttccgagc gcagcgagga atctcgggtc
     5701 catgtggcga tgaggtccct cgctgcgctc gggacaacag gcagttaggg gtttcgccgt
     5761 gcctgccgtg ttcgccgtgg tgaaaaacat tcagaccccc gaactgcaag ccgcgccagg
     5821 aaacaaaaag gcccgcatcg ctgcgggcct tttttgcaac tgacgcaaac agaagcgatc
     5881 agtaatccag gtagtcggtg accgccaccc acttgccgtt ctggatctgg ccgatgcgcg
     5941 aacgatcgtt gccgaggcgg ttcgtggaag tgaagcgata gcccgggctg ccgaacatgt
     6001 cgcgcgggaa ccacatcgac tccatcgtac tgatcagcgt gtcggtcgtc aggttcgggc
     6061 cggccttttc cgcggcagcg gcgaacatcg tgatcatcga gtagccatag gcggagaaca
     6121 cggtcggatc ttccttgaag cgatccttgt agcgggcgcc ccagtcgcgt acgttcttcg
     6181 acgcgtcatc gaggtagggc acgccgatct ggaaggccga atagaagccg tccatcgcct
     6241 tgccgccaag ccggtggatc agttcggtgt aggaagcgct ggtgccgatg aaattcgggt
     6301 tccagccgga cttgcgtgct tcaccgatca cgccgatggt ttcacgaatg atcgtaccca
     6361 tgacgacggt gtcgcatccg gaagcgcgca tgcgtgccac ctgcgaagaa aagtccgtcg
     6421 cgccgcgctt gtaggtcgtg cgctcggtcc agttcagccc aagtttctgc aggccttcct
     6481 cggcgccgcg catcacttcg aggccgaatt catcgtcctg atagacgatg cagaagctct
     6541 tcgcgccgcg ttccttcgcc atgaattgca cgccgctgcg cgcctggttg tagtacgggg
     6601 cggcgaacga aaacttcagg cggtggaagg gctcgtacat ttcccgcgcg gcggtcagcg
     6661 ggaacatgtt cggcacattg cgttcgaaca ggatcggaag cgtagccatc acgaccggag
     6721 tgccgatggt gc
//
LOCUS       DATLGF010000026         3859 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_49190,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000026 DATLGF010000000
VERSION     DATLGF010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49190"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /gene="aceA"
                     /locus_tag="VM406_00805"
     CDS             <1..899
                     /gene="aceA"
                     /locus_tag="VM406_00805"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HVL74526.1"
                     /translation="RGINPGDEGFIDYFLPIVADAEAGFGGVLNAFELMKNMIAAGAA
                     GVHFEDQLAAVKKCGHMGGKVLVPTQEAIEKLVAARFAADVMGVPTIVLARTDAEAAN
                     LITSDHDANDKPFLTGERTQEGFYRVKNGLEQAISRGVAYAPYADLVWCETGTPDLGF
                     AREFAQAVHEKCPGKLLSYNCSPSFNWKKNLDDKTIAEFQQKLSELGYKYQFITLAGI
                     HINWFNTFQFAHAYARGEGMKHYVNMVQEPEFAAREKGYTFVSHQQEVGAGYFDEVTT
                     VIQGGSSSVKALTGSTEEEQFH"
     gene            complement(980..2344)
                     /locus_tag="VM406_00810"
     CDS             complement(980..2344)
                     /locus_tag="VM406_00810"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HVL74527.1"
                     /translation="MMRSISIRLAVAFGLVALVVFSLSGIALYQALAVTLDRQQEADL
                     RDKLDVVEKLAGEIRTLERWDELRKTLATITSTDGNTRFWIACENTDLSYGETYPDHP
                     PGSKEIRTLRLEDDRYPMKTVGKTLRPMHEFPEVHLLVGVSSAPAAHTLQNFALALVL
                     ISLCGIALVALVGYRIARFGLRPLADLSAGAQALNPRQPSQRLSLSPAVRELEPLVES
                     FNGALARLEGAYAQLEGFSADVAHELRTPIGNMIGMTQVALTRERSRDELRETLESNL
                     EELERLRAIVNDMLFLARADRGEKAGPLEQVALAQEVAKTIDFFEPLLEEKSLSIEVR
                     GEVEAPVEKSLFRRALSNLLQNAIQHAPAGARLVVELRREPEHVAVAVANPGDTIPAE
                     HLPRLFDRFYRVDAARASSTDSHGLGLAIVHAIARMHGGGVFAESAGGINRIGLTLLP
                     ACPG"
     gene            complement(2341..3027)
                     /locus_tag="VM406_00815"
     CDS             complement(2341..3027)
                     /locus_tag="VM406_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HVL74528.1"
                     /translation="MKILLIEDEPKTADYLRKGLTEHSYVVDVARTGVDGRHLALVND
                     YDAVVLDVMLPGVDGFGVLEALRIHRQTPVIMLTARDRVEDRVRGLQAGADDYLVKPF
                     SFLELLARLQALVRRGKPQEAAQFQIADLHVDLIARKATRSGVRLDLTSKEFALLCAL
                     GRRRGQILSKTEIAELVWDMNFDSNTNVIEVAIKRLRTKMDGPFEPKLLHTVRGMGYV
                     LEHRVEEAAR"
     gene            3181..3771
                     /locus_tag="VM406_00820"
     CDS             3181..3771
                     /locus_tag="VM406_00820"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HVL74529.1"
                     /translation="MRFTSAIRQIGSASAAALFAASAFAAPVAGNYKVDPAHSVAYFE
                     VGHAGGISRFMGRFNDISGDLVVDTPEKSKVTVNIKTDSIDANHDGLNRHLRSPDFFS
                     AVQFPTMSFTSTAVTLNPAGEGTLAGNLTIRGVTKPVTFKLRHIGSGSGPRGDTRVGY
                     TATSTIKRSDFGMTYGIPQAATDEVELRINIEAIKQ"
BASE COUNT          691 a         1290 c         1196 g          682 t
ORIGIN      
        1 cgcgcggcat caacccgggc gacgaaggct tcatcgacta cttcctgccg atcgttgctg
       61 atgcggaagc cggtttcggc ggcgtgctga acgccttcga actgatgaag aacatgatcg
      121 ccgcgggcgc agccggcgtg cacttcgaag accagctggc tgccgtgaag aagtgcggcc
      181 acatgggcgg caaggtgctg gtgccgaccc aggaagcgat cgagaagctg gtagccgcgc
      241 gattcgccgc cgacgtgatg ggcgtgccca ccatcgtgct ggcccgcacc gatgccgaag
      301 ccgcgaacct gatcacctcg gaccacgacg ccaacgacaa gcccttcctg accggcgaac
      361 ggacccagga aggtttctac cgcgtcaaga acggcctgga gcaggcgatc tcgcgtggtg
      421 tcgcctacgc tccgtacgcc gatctggtgt ggtgcgaaac cggtacgccg gatctgggct
      481 ttgcccgcga attcgcgcag gctgtccatg aaaagtgccc gggcaagctg ctgtcctaca
      541 actgctcgcc gtcgttcaac tggaagaaga acctggacga caagaccatc gccgaattcc
      601 agcagaagct gtccgagctg ggctacaagt accagttcat cacgctggcc ggcatccaca
      661 tcaactggtt caacaccttc cagttcgcgc atgcctatgc acgcggcgaa ggcatgaagc
      721 actacgtgaa catggtgcag gagccggaat tcgctgcacg cgagaagggc tacaccttcg
      781 tctcgcacca gcaggaagtc ggcgccggct acttcgacga agtgaccacc gtgatccagg
      841 gcggctcctc gtccgtgaag gccctgaccg gctcgaccga agaagagcag ttccactgat
      901 ccgcgcgccc ggcacgccgg gccgcaaaag attctccgta ggacgttttt cagccgcacc
      961 tcccggtgcg gctgtttttt tatccggggc aggcgggcag gagggtcagt ccaatccgat
     1021 tgatgccgcc ggcgctttcc gcgaagacgc cgccgccatg catcctggcg atcgcatgca
     1081 cgatggccag ccccaggcca tggctgtcgg tgctcgaagc gcgcgccgca tcgacgcgat
     1141 agaagcggtc gaacaggcgc ggcaggtgtt cggcggggat tgtatcgccg ggattggcaa
     1201 ccgccaccgc cacatgttcc ggctcccgtc tgagctcgac gacgaggcgt gcaccggcag
     1261 gcgcgtgctg gatcgcgttt tgcagcagat ttgacagcgc tcggcggaac agggacttct
     1321 ccaccggcgc ctccacctcg ccgcgcactt cgatactgag cgatttctcc tccagcagcg
     1381 gctcgaaaaa gtcgatcgtt ttagccactt cctgcgccag cgccacctgt tcgaggggcc
     1441 ctgccttctc gccgcggtcg gcgcgcgcga ggaacagcat gtcgttgacg atggcgcgca
     1501 gccgttcgag ttcttccagg ttcgattcca gcgtctcgcg cagttcatcg cgactgcgct
     1561 cgcgggtgag ggcgacctgc gtcatgccga tcatgttgcc gatcggcgtg cgcagctcgt
     1621 gcgcgacatc ggcgctgaat ccttcgagct gggcataagc accctccagc cgggcgagcg
     1681 ccccgttgaa ggattccacc agcggctcca gctcacgcac ggccgggctc aggctcaggc
     1741 gctgggaggg ctgcctcggg ttcagggcct gcgcgccggc cgagagatcg gccagcggac
     1801 gcaggccaaa gcgcgcgatc cggtagccga ccagcgccac cagcgcgatc ccgcacagcg
     1861 agatcagtac cagcgccagc gcaaagttct gcagcgtgtg cgcggccggc gcgctgctca
     1921 cgccgaccag cagatgcact tcggggaatt cgtgcatcgg gcgcagcgtc ttgcccacgg
     1981 tcttcatcgg atagcggtcg tcctccaggc gcagcgttct gatttccttg ctgccgggcg
     2041 ggtggtcggg gtaggtctcg ccatagctca ggtcggtgtt ctcgcaggcg atccagaagc
     2101 gggtattgcc gtcggtggag gtgatcgtcg cgagcgtctt gcgcagctcg tcccagcgtt
     2161 ccagcgtcct gatctcgccg gccagctttt ccacgacatc gagcttgtcg cgcagatcgg
     2221 cttcctgctg gcggtccagc gtgacggcga gcgcctggta cagcgcaatg ccggacaatg
     2281 agaacaccac cagcgcgacc aggccgaagg ccacggccag gcgtatcgag atcgagcgca
     2341 tcatcgcgca gcttcctcga cgcggtgctc cagcacgtag cccatgccgc gcaccgtgtg
     2401 cagcagcttc ggctcgaacg ggccatccat cttcgtgcgc agccgcttga tggcgacctc
     2461 gatcacgttg gtattgctgt cgaagttcat gtcccatacc agctcggcaa tctcggtctt
     2521 ggacaggatc tggccgcgcc gccgtcccag cgcgcacaac agcgcgaact ccttcgatgt
     2581 cagatcgagc cgaacgccgc tgcgcgtggc cttgcgcgcg atcaggtcca catgcaggtc
     2641 ggcgatctgg aactgcgccg cttcctgcgg cttgccgcgc cgaaccagcg cctgcaggcg
     2701 cgccagcagt tcgaggaagg agaatggctt gactagatag tcatccgcgc ccgcctgcag
     2761 accccgcacg cggtcctcga cgcggtcgcg cgcggtcagc atgatgaccg gggtctgccg
     2821 gtggatacgc agcgcttcca gcacgccgaa gccgtccacg ccggggagca tcacgtccag
     2881 cacgaccgcg tcatagtcgt tgaccagcgc caggtggcgg ccgtccacgc ccgtgcgcgc
     2941 gacgtcgacc acgtagctgt gttcggtcag ccccttgcgc aggtagtccg cggtctttgg
     3001 ttcgtcttcg atgagcagga tcttcatgcg cgaatatgta ccagaaaatg ccttggcggc
     3061 agattacaaa tttgtcaggc gcgcgacagc atggtgtcgg cgtcgtttca ctagacttcg
     3121 gcagtcgtcg ctgcaagcca cgcgacggat caatccgaca accattgacg aggtctcatc
     3181 atgcgattca cttccgccat ccgccagatc ggctcggcat ccgccgccgc actgtttgcc
     3241 gcctcagcgt tcgccgcccc ggtggcgggc aactacaagg tcgatcccgc gcactcggtc
     3301 gcttatttcg aagtcggcca tgctggcggc atcagccgct tcatggggcg cttcaacgac
     3361 atctccggcg atctcgtggt cgatacgccc gagaagagca aggtcacggt gaatatcaag
     3421 accgacagca tcgacgccaa ccatgacggc ctgaatcgcc acctgcgcag cccagacttc
     3481 ttcagcgccg tacagttccc gacgatgagc tttacgtcca cggcggtcac gctcaatcct
     3541 gccggcgaag gtacgctggc cggcaacctg accatccgcg gcgtgaccaa gccggtcacg
     3601 ttcaagctgc gccatatcgg ctccggcagc ggcccgcgcg gcgacacccg cgtcggctat
     3661 accgccacct cgacgatcaa gcgcagcgat ttcggaatga cctacgggat cccgcaggcg
     3721 gcgaccgacg aggtcgaact gcgcatcaac atcgaagcga tcaagcagta attccatccg
     3781 cggcgggagt gatcccgcct ttgatcatgt cagcttcctt cattctcccc ctgctgctgc
     3841 aaagcgcgct gctgccgtt
//
LOCUS       DATLGF010000027         3296 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_128099, whole genome shotgun sequence.
ACCESSION   DATLGF010000027 DATLGF010000000
VERSION     DATLGF010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128099"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VM406_00825"
     CDS             <1..560
                     /locus_tag="VM406_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HVL74530.1"
                     /translation="PEHMYFVLVDAGRTSLVGSEFQDMLRCIRCGACMNHCPVYQKIG
                     GHSYGWVYPGPMGSVLTPSYAGIENALDLPQASTLCGECHVVCPVKIPLPDLLRKLRE
                     KQVAMHLRPATERLGFAVWAFFAKRPALYRIASRTGVRVLRMIAGGRKTISKLPLVHG
                     WTDYRDMPAPSGRTFQELYRKRKQK"
     gene            668..1705
                     /locus_tag="VM406_00830"
     CDS             668..1705
                     /locus_tag="VM406_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HVL74531.1"
                     /translation="MKPRSRLFPLALLALLLGVAALALIRPGGGAAASTANFSLLNVS
                     YDPTREFYTDFNAAFARHWKAQTGQDVHIQQSHGGSGKQARSIIDGLAADVATLALAG
                     DTDALHQHGGLIPADWQQRLPHNSSPYTSTIVFVVREGNPKRIRDWDDLVRSDVQVIT
                     PNPKTSGGARWNYLAAWEYAKRKYGGEAQAQDFVTRLYRNVPVLDAGARGSSVTFAQR
                     GQGDVFLSWENEAHLLEKEFGSRFDIVYPSLSIRAEPAVTVVDRNVDRKGTRAVAEAY
                     LQYLYSDEGQELAARHFFRPVNEAVQARHAGRFPHIELFDIDAAFGGWGEAAAKHFAD
                     GAIFDQIYAPR"
     gene            1806..2804
                     /locus_tag="VM406_00835"
     CDS             1806..2804
                     /locus_tag="VM406_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HVL74532.1"
                     /translation="MKTVIRSAFIALAFAASSVASAQTSLLNVSYDVAREFYKDFNTA
                     FVAHYKKSTGKDIKVDQSHAGSSAQARAVADGLDADVVTMNTTTDVEFLAERGVVAKD
                     WAKRFPHNAAPTTSTMLFLVRNGNPKNIKDWEDLIKPGVQVIVVNPKTGGNGRMAYLA
                     AWGYVKSKGGTDQQAQDFVRALYKNVPVLARGGRDATGIFLQRNIGDVLITFESEVVS
                     VEREFGAGKVDAVHPSVSIVAENPVAIVERTVNKKGTREAAQAYLDFLYSDEGQEIAA
                     KHNIRPRNPAILAKYSNNLKPIKLFTVAEYFGSLSEAQKVHFNDGGLFDKIYTAGR"
     gene            2804..>3296
                     /locus_tag="VM406_00840"
     CDS             2804..>3296
                     /locus_tag="VM406_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HVL74533.1"
                     /translation="MAGAPTTVLAGGAAAGAPARGARRGSARVLPGFNLTLGYTIFYL
                     SLIVLIPLTTLAFKTFTLTWPEFWEAVTAPRVLASYRLTFGASLFAATVNLFFGLLVA
                     WVLVRYTFPGKKIVDALVDLPFALPTAVAGISLSAILAGNGWIGQFLEPYGIQIAFTP
                     AGVV"
BASE COUNT          604 a         1072 c         1007 g          613 t
ORIGIN      
        1 gccccgagca catgtatttc gtgctggtcg acgccggccg caccagcctg gtcggcagcg
       61 aattccagga catgctgcgc tgcatccgct gcggcgcctg catgaaccat tgtccggtct
      121 accagaagat cggcggacac agctacggct gggtctatcc cgggccgatg ggcagcgtgc
      181 tcacgccctc atatgctggc atcgagaacg cgctcgacct gccccaggct tcgacgctgt
      241 gcggcgaatg ccatgtggtg tgtccggtga aaatcccgct gccggacctg ctgcgcaaat
      301 tgcgcgagaa gcaggtggcg atgcacttgc ggccggccac cgaacgtctt ggttttgctg
      361 tatgggcgtt ctttgcgaag cggccggcgc tgtaccggat cgccagtcgc acaggggtcc
      421 gcgtgctcag gatgattgcc gggggcagga agacgatctc gaagctgcct cttgtgcacg
      481 gctggaccga ctatcgtgac atgccggcgc catccggcag gacgttccag gagctgtaca
      541 ggaaacggaa gcaaaagtag ttatatacca gaaagtaata agtaacttag tttttattcg
      601 tttctgtttt atagatctgg tttcatcatc cggtgttcag gacgactcct cacaccggtt
      661 gcaccagatg aagccaagat cacgactctt tccgctggcc ctgctggcgc tgctgctggg
      721 agtggccgcg ctggcgctga ttcgcccggg tggcggggcc gcggcctcca ccgcgaattt
      781 ctcgctgctc aacgtctcct acgatcccac gcgcgagttc tacaccgact tcaatgccgc
      841 attcgcgcgt cattggaagg cgcagaccgg ccaggacgtg catatccaac aatcccacgg
      901 cggctccggc aagcaggcgc gctctatcat cgacgggctg gcggccgacg tggcgacgct
      961 ggccttggcg ggcgataccg atgcgctgca tcagcacggc ggcctgatcc cggccgactg
     1021 gcagcagcgc ctgccgcaca attcttcccc ctacacctcg accatcgtgt tcgtggtgcg
     1081 cgaaggcaat ccgaagcgca tccgcgactg ggacgacctc gtgcgctccg acgtgcaagt
     1141 gatcaccccg aacccgaaga cctcgggcgg ggcgcgctgg aactatctcg ccgcctggga
     1201 atacgcgaaa cgcaagtacg gcggcgaggc gcaggcgcaa gacttcgtca cgcgtctgta
     1261 ccgcaacgtg ccggtgcttg atgccggcgc gcgcggttct tcggtcacct tcgcccagcg
     1321 cggacagggc gatgtcttcc tgtcgtggga gaacgaagcc cacctgctgg agaaggaatt
     1381 cggcagtcgc ttcgacattg tctatccgtc gctgtccatc cgcgccgaac ccgccgtcac
     1441 ggtcgttgac cgcaatgtcg accgcaaggg cacgcgggcg gtagccgagg cttatctgca
     1501 atacctgtac agcgacgagg ggcaggaact ggcggcgcgc catttcttcc gcccggtgaa
     1561 cgaagccgtg caggcgcggc atgcgggacg ctttcctcac atcgaactct ttgacatcga
     1621 tgccgccttc ggcgggtggg gcgaggccgc agcgaagcat ttcgccgacg gagcgatctt
     1681 cgaccagatc tatgcgccga gatagctgtc cttatgccac caaagcataa gaaaacaaga
     1741 acaaagtagt ttgggtttgt aaggccagtc ggcagaatgc catccgttca tattggggat
     1801 tttcaatgaa aaccgtaatc cgctccgctt tcatcgcgct ggccttcgcc gccagttccg
     1861 ttgcttccgc ccagacctcg ctgctgaacg tctcgtatga cgtcgcgcgc gagttctaca
     1921 aggatttcaa caccgccttc gtcgcgcatt acaagaagtc gacgggcaag gacatcaagg
     1981 tcgaccagtc gcatgccggc tcttccgcgc aggcgcgcgc agtggccgac ggcctcgacg
     2041 ccgacgtggt gacgatgaac accaccaccg acgtcgagtt cctcgccgaa cgcggcgtgg
     2101 tggccaagga ctgggcaaag cgcttcccgc acaatgccgc gccgaccacc tcgaccatgc
     2161 tgttcctggt tcgcaacggc aacccgaaga acatcaagga ctgggaagac ctgatcaagc
     2221 cgggcgtcca ggtcatcgtc gtcaacccga agaccggcgg caacggccgc atggcctatc
     2281 tggccgcctg gggctacgtg aagagcaagg gcggcaccga tcagcaggcg caggacttcg
     2341 tgcgcgcgct gtacaagaac gtgccggtcc tggcgcgcgg cggtcgtgac gccaccggca
     2401 ttttcctgca gcgcaatatc ggcgacgtgc tgatcacctt cgaatccgaa gtcgtatcgg
     2461 tcgagcgcga attcggcgcc ggcaaggtcg atgcggtgca cccgtcggtc tccatcgtgg
     2521 ccgagaaccc ggtcgccatc gtcgaacgca cggtcaacaa gaagggtacg cgcgaagcgg
     2581 cccaggccta tctcgacttc ctgtactccg acgaaggcca ggaaatcgcg gccaagcaca
     2641 acatccgtcc gcgcaacccg gcgatcctgg cgaagtacag caacaacctg aagccgatca
     2701 agctgttcac ggttgccgaa tatttcggtt cgctgtccga agcgcagaag gtccacttca
     2761 atgacggtgg cctgttcgac aagatctaca cggcaggacg ctgatggccg gcgctccgac
     2821 caccgtactg gcaggcggag ccgctgccgg agccccggcg cgcggcgcgc gccggggtag
     2881 tgcgcgtgtg ctgcccggct tcaacctgac gctgggctac acgatcttct acctgagcct
     2941 gatcgtcctg attccgctga ccacgctggc cttcaagacc ttcacgctga cctggcccga
     3001 gttctgggaa gccgtcaccg caccccgcgt gctggcttcc taccgcctga ccttcggcgc
     3061 ctcgctgttt gcggcaaccg tcaatctgtt cttcggcctc ctggtggcct gggtgctggt
     3121 gcgctacacc ttccccggca agaagatcgt cgacgccctg gtcgacttgc cgttcgcgct
     3181 gcccaccgcg gtcgccggca tctcgctttc ggcgatcctc gccggcaacg gctggatcgg
     3241 ccagtttctc gagccgtacg gcatccagat cgcgttcacg ccggccggtg tcgtgg
//
LOCUS       DATLGF010000028         9599 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_17580,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000028 DATLGF010000000
VERSION     DATLGF010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 9599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9599
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17580"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2049
                     /locus_tag="VM406_00845"
     CDS             <1..2049
                     /locus_tag="VM406_00845"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HVL74534.1"
                     /translation="EQIANLFERVIQDERLYAVAFCGPAGDLVHTSRTFPAALGCTQS
                     AGAGNPGSVISLPDGPVHVSRSAVSEDQQTLGHLVLVHDMSFISRRSADTRNYVIALF
                     ALLGVVVSLLTVFIAHLSWRGWVRGVKGLLRGDLLRPFAPPAPELQPLVGDLRALLHE
                     INLERQGPSDPLQTWTPEKLRALLQRELAGDEVLVVSNREPYIHVSTARGIEIKRPAS
                     GLVTAVEAVMRACSGTWIAHGAGSADRQVVDSHARVPVPPSNPSYTLRRVWLTKEEEQ
                     GYYYGFANEGLWPLCHIAHVRPVFRSSDWEQYEKVNRRFADAVIREARTDDPVVLVQD
                     YHFALLPRMVREALPKATIITFWHIPWPNPESFGICPWRAEILEGLLGSTILGFHTPF
                     HRKNFLETVDRYLEARIEAEDSTISYGGKLTRVEAYPISIAWPQADAAAASVDECRSL
                     IRSELSLPQNALLGIGVDRMDYTKGILERFQAVERMLELFPEMVGRFTFIQIAAPSRS
                     SLDEYQNFEARVRALAQRINQRFSNGSYQPIMLKAEHHDQDALFRYYRASDVCLVTSL
                     HDGMNLVAKEFIATRDDEQGALVLSQFTGAARELHEALIVNPYHIEQSAEALYRALTM
                     PAAEQRERMRSMRSLVKDFNVYRWAGRMLIDAARLRQRERVMSRIDSHSGRTQLRRVV
                     "
     gene            2046..2843
                     /gene="otsB"
                     /locus_tag="VM406_00850"
     CDS             2046..2843
                     /gene="otsB"
                     /locus_tag="VM406_00850"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HVL74535.1"
                     /translation="MKHGALPFFEHGCAELDEMAAAGLLCAFDFDGTLAPIVTQPDQA
                     RTPLPVLQRLNELARFAPTAVITGRALADMRERLGFMPDYLIGNHGLEGAPGVDEDKL
                     RHAELCRRWKAALEAALQAHAPAVWIEDKTYSLSLHYRLAREQRAAEDALRTLLATLE
                     PPPRVIDGKCVFNVLPADALDKGRALEALMRDCGARCALYIGDDVTDEDVFRLRRPDI
                     LTVRIGRSQDSAARYYLNHRVDLIALLDRLLRRYTEMGSTPRTGSAR"
     gene            2840..3223
                     /locus_tag="VM406_00855"
     CDS             2840..3223
                     /locus_tag="VM406_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74536.1"
                     /translation="MRRTPIPEDVRSFLRSDIPTVPHLEALLLMRSTPDAQWNAVQLA
                     RRLYLSEVTASGLLRDLRSRGLVTPAEHGEACRYAPADSRTCALIDRLAQLYSTNLVG
                     ISNLIHSVAQTDRTARPPGSDRRGN"
     gene            complement(3147..3701)
                     /locus_tag="VM406_00860"
     CDS             complement(3147..3701)
                     /locus_tag="VM406_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74537.1"
                     /translation="MPFAFLFAMAALCVRLRALRRAQATCARRYWACIVAAHAALAYG
                     VLLLTVLTIDMLSAGRWGVPLEWPAFASLWSSSGNRTVLLLLDVASALLLSLTAAAAA
                     ARFGYVNAANVWHPPRRRDARRGREAMPGLPDPHPSFGAGAAAQSRAGDRVERGTVRV
                     SCRACRCQEAAPCDRSARRNGSDC"
     gene            3887..4303
                     /locus_tag="VM406_00865"
     CDS             3887..4303
                     /locus_tag="VM406_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HVL74538.1"
                     /translation="MPTLEEKAAFARRLQLALRRSPEPVQGATELALRFNLRHGGSAI
                     SPQTAHKWLSGRAIPSDDKLATLAAWLNVNPHWLHYGPPPATVLQAREQGQPYEVAEP
                     AVSQEALALARKIQLLGPHRRYLIEELVEQLLQEGG"
     gene            4429..4502
                     /locus_tag="VM406_00870"
     tRNA            4429..4502
                     /locus_tag="VM406_00870"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4461..4463,aa:Gly,seq:ccc)
     gene            4741..6666
                     /locus_tag="VM406_00875"
     CDS             4741..6666
                     /locus_tag="VM406_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HVL74539.1"
                     /translation="MLTDMTVRQAKATGKPYTIADFDGLSLFVSATGAKTWHFRYTWI
                     GQRARISLGCYPELSLRDARELRDEARSLVAKGINPRIERKQKRQAIMLAGENTFMTV
                     YEQWMEHRQLTLEEGRQSSLEQIRRVFKKDVFPYLKRLTIYEVTRPHLLEVIGRIEKR
                     NSLSVAEKVRTWFKQLFDYAMVVIPAMETNPATDLHVVAVPLPPVEHNPFLRMAELPE
                     FLQTLRKYSGMLKTQLAIRLLLLTGVRTGELRLATPDQFDLDRGLWIIPVMSLKQRKM
                     LTRKKRKRPTDIPPYIVPLSVQAREIVRHMLDDFKPVQKYLFAGVKRLTDRMSENTLN
                     AALKRMGYGDRLTGHGIRATISTALNELGYPKVWVDAQLSHADPNRISATYNHAEYVE
                     QRRVMMQDWADRLDLFEQNQVQTASMHLTIHLQGLPTIAGQQTTPLPTPGQHASILLV
                     APTEQSMPTGTLPTQRLSAVEMPEYAQPKLSDLQRERLKLLEIFEGPDNLVVADYAKL
                     AGKSRRWITYEIQAGNLLSIQLGNKGQRVPVWQLDPLKRQLVQAILKQTPRGVDTWDI
                     YHALLRPHDALGSRPPIEAVTPENMKIAVRVVVEQCSQLDEPGAPTPYPIEVRQSVQR
                     VIQNANAADAALPMGND"
     gene            complement(6725..7366)
                     /locus_tag="VM406_00880"
     CDS             complement(6725..7366)
                     /locus_tag="VM406_00880"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVL74540.1"
                     /translation="MSAPDRSPILKLAVSADTLSPRLASLLALQRAEEPETIILLQEV
                     AAKDLARGLEDGAYDAAIALVATTTVFAMKAQPLWADELALAVPLRSPLLAYAEVPLD
                     ALLHYPLLQWCLRACEALSQQVDARLGPEKHSAQEVTSFDLLAVLVAAGYAVGIAPRS
                     QITQVRGWGVAMRPLAEGSYVVRTQLLQPSRECLPAVERFAERAAKVAATEAG"
     gene            7473..7766
                     /locus_tag="VM406_00885"
     CDS             7473..7766
                     /locus_tag="VM406_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVL74541.1"
                     /translation="MQKRTVQRGRPVGSTTYEPEPALAFGQAVRATRMEQGMAQEELA
                     AQAAIERSHMGKIERGEHMPTLALILRIAIALNRSAAELIAATEENLRSGPRT"
     gene            complement(7794..8690)
                     /locus_tag="VM406_00890"
     CDS             complement(7794..8690)
                     /locus_tag="VM406_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL74542.1"
                     /translation="MELRHLRCFVVLAEELHFTRAAERLHIEQSPLSRTIKELEDELD
                     VLLFDRDRRGTRLTQAGEVFLQDVHRVFSTLELARENVKAVSSGYLGILRIAVPRGCI
                     GPRLSTFLARCREEDPAVEIRLTEASLADQLRGLRSGNFNLGFAYTADVGDGIIAEPI
                     WVDSVVVAVAARHPLLVHKQIPLEDLIRYPLVVADRRLFGNWDCEVGQLLRDAAVKLD
                     VEYVASVDMMLTLVAAGYGVGFASAARIGVLNRPDIVIRPLATPNVIHTTYLLRPESD
                     TDTPAAISLDRFIARLHEQIDE"
     gene            complement(9142..>9599)
                     /locus_tag="VM406_00895"
     CDS             complement(9142..>9599)
                     /locus_tag="VM406_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HVL74543.1"
                     /translation="SMAPAPTTTSGMEETPSGEHFVAWLRRRIEERGLIINDAKALVH
                     TVADTVYLVSPGVFQRYAQEHPETALLAKQDQMADWQWVQKRFEKLQLHRKQQNGLNI
                     WTCTVTGPRKSRRLHGYLLEDPRVLFIDLPPNNPYLSLSPSTDSVCQPS"
BASE COUNT         1918 a         3094 c         2928 g         1659 t
ORIGIN      
        1 gaacagattg ccaacctgtt cgagcgcgtg atccaggacg agcgtctgta cgcggtcgca
       61 ttctgcggcc cggccggcga cctcgttcat accagccgca ccttccccgc cgcgctgggc
      121 tgcacgcagt ccgccggcgc cggcaacccg ggatcggtga tcagccttcc cgatggtccg
      181 gtccacgtgt cgcgcagcgc cgtgagcgag gaccagcaga ctttgggaca cctggtcctg
      241 gtgcacgaca tgagcttcat cagccgccgc agcgccgata cgcgcaacta cgtgatcgcg
      301 ctgttcgcgc tgctcggcgt agtggtctcg ctgctgacgg tgttcatcgc ccatctgagc
      361 tggcgcggct gggtgcgcgg cgtcaagggc cttttgcgcg gcgatctgct gcggccgttc
      421 gccccgccgg cgccggagct gcagccgctg gtcggcgacc tgcgcgcgct gctgcatgag
      481 atcaacctcg aacgccaggg tccgagcgat ccattgcaga cctggacgcc ggaaaagctg
      541 cgcgcgctgc tgcagcgcga gctggccggc gacgaagtgc tggtggtctc caaccgcgaa
      601 ccctacatcc atgtctccac cgcgcgcggc atcgagatca agcggccggc cagcggcctc
      661 gtcaccgcgg tggaagcggt catgcgcgcc tgctccggca cctggatcgc gcacggggcc
      721 ggctccgcgg acaggcaggt ggtcgacagc cacgcccggg ttccggtccc cccgtccaat
      781 ccgagctata cgctgcgccg cgtctggctg accaaggaag aggaacaggg ctactactac
      841 ggcttcgcga acgagggcct gtggcccctg tgccatatcg cgcatgtgcg gccggtgttc
      901 cgctcgtcgg actgggagca gtacgagaag gtcaaccggc gcttcgccga cgcggtgatc
      961 cgcgaggcgc gcaccgacga tccggtcgtg ctggtgcagg actatcactt cgcgctgctg
     1021 ccgcgcatgg tgcgcgaggc gctgcccaag gccaccatca tcacgttctg gcacattccc
     1081 tggccaaatc cggagtcctt cggcatctgc ccgtggcgcg ccgaaatcct tgaaggactg
     1141 ctcggcagca ccatcctcgg cttccacacg cccttccatc gcaagaattt cctggagacc
     1201 gtcgaccgtt acctggaagc gcgcatcgag gcggaagact cgacgatctc ctatggcggc
     1261 aagctgaccc gcgtggaggc gtatccgatc tcgattgcct ggccgcaggc cgacgcagcg
     1321 gcggcttcgg tggacgaatg ccggtcgctg atccgcagcg aactctcgtt gccgcagaac
     1381 gcgctgcttg gcataggcgt cgaccggatg gactatacga agggcatcct cgagcgcttc
     1441 caggcggttg aacgcatgct cgaactgttt ccggagatgg tcgggcgctt caccttcatc
     1501 cagatcgccg cgccgagccg ctcctcgctc gacgagtacc agaacttcga agcgcgggtg
     1561 cgcgcgcttg cccagcgcat caaccagcgc ttctcgaacg gcagctacca gccgatcatg
     1621 ctcaaggccg agcatcacga ccaggatgcg ctgttccgct actaccgcgc ctccgacgtc
     1681 tgcctggtca ccagcctgca tgacgggatg aacctggtgg ccaaggaatt catcgccacg
     1741 cgcgacgacg agcagggggc gctggttctt tcgcagttca ccggcgccgc gcgcgaattg
     1801 catgaagcgc tgatcgtaaa tccgtatcac atcgaacaga gcgccgaggc gctgtaccgt
     1861 gcgctgacga tgccggcggc cgaacagcgc gagcgcatgc gcagcatgcg ctcgctggtg
     1921 aaggacttca acgtctaccg ctgggccggc cgcatgctga tcgacgcggc gcgcctgcgc
     1981 cagcgcgagc gggtgatgtc gcgcatcgat tcgcacagcg ggcgcacgca actgcgcagg
     2041 gttgtatgaa acacggcgcc ctgcccttct tcgaacacgg ctgcgcggag ctggacgaga
     2101 tggccgccgc tggcctgttg tgcgcgtttg actttgacgg cacgctggcg cccatcgtca
     2161 cgcagcccga ccaggcccgc acgccgcttc cggtgctgca acgcctgaac gagcttgcac
     2221 gctttgcgcc gaccgccgtg atcaccggcc gcgcgctcgc cgacatgcgc gagcgccttg
     2281 gcttcatgcc ggactatctc attggcaatc acgggctcga aggtgctccg ggcgtggacg
     2341 aggacaagct gcgccatgcc gagctgtgcc gtcgctggaa ggcggcgctg gaagcggcgc
     2401 tgcaggcgca cgcaccggcg gtctggatcg aggacaagac ctactcgctg tcactgcact
     2461 atcggctcgc gcgtgagcag cgcgccgccg aggacgcgct gcggaccctg ctcgcgacgc
     2521 tcgagccgcc tccccgcgtg atcgacggga agtgcgtgtt caacgtgctg ccggcggatg
     2581 cgctggacaa gggtcgtgcg ctggaagccc tgatgcgcga ttgcggcgcg cgctgcgcac
     2641 tgtacatcgg cgacgacgtg accgacgagg acgtgttccg gctgcgccgg cccgacatcc
     2701 tgaccgtgcg catcggccgt tcccaggaca gcgccgcccg ctactacctg aatcaccgcg
     2761 tcgacctgat cgcgctgctg gaccggctgc tgcgccgcta cacggagatg ggaagcacgc
     2821 cgcgcaccgg aagcgcgcga tgaggcgcac accgataccc gaggacgtgc gcagcttcct
     2881 gcgctcggac atccccacgg ttccgcatct ggaggccctg ctcctgatgc gaagcacgcc
     2941 cgatgcgcag tggaatgccg tccagctcgc gcgccgcctc tatctgagcg aagtgacggc
     3001 gagcggcttg ctgcgtgact tgcgcagccg cggtctcgtg acgccggccg aacacggcga
     3061 ggcctgccgc tatgcgccgg cggattcgcg cacctgcgcg ctgatcgacc gcctcgccca
     3121 actgtattcg accaacctgg tcgggatcag caatctgatc cattccgtcg cgcagaccga
     3181 tcgcacggcg cggcctcctg gcagcgacag gcgcggcaac tgacgcgcac cgtcccgcgt
     3241 tcgacgcgat cgccggcacg agactgcgcg gcagcgcctg cgccaaatga gggatgcggg
     3301 tcgggcagcc cgggcatcgc ttccctgcca cgccgtgcgt cccggcggcg cggcgggtgc
     3361 cagacgttgg ccgcgttcac gtaaccgaag cgcgccgccg ccgcggccgc cgtcagcgaa
     3421 agcagcagcg cacttgccac gtcgagaagc agcagcaccg tccggtttcc ggaagaggac
     3481 cacaggcttg cgaacgccgg ccattcgagc ggaacacccc agcgacccgc gctcagcata
     3541 tcgattgtca gcacggtcag gagcagcacc ccatacgcga gggccgcgtg cgcggccacg
     3601 atgcacgccc agtagcgacg cgcgcacgtc gcctgcgcac gccgcagcgc acgcagccgc
     3661 acgcacagcg cggccatcgc gaacaggaag gcgaatggca ccagaaatgg aaggacaagg
     3721 tagagcaccg cgtcgacctc tcaggaagaa atctgctccg cacgtccggg ccaccgcccg
     3781 aactcgccgc atcttatccc aagcaggcga tgttttaaac tgttcaggct atttttatcc
     3841 tggttcgttg acctacgcct cgacattgag gtccagaatg ccggccatgc cgacactcga
     3901 agaaaaagcc gcatttgcgc gtcgcctgca actggcattg cggcgcagcc cggagccggt
     3961 ccagggagcg accgagcttg cgctgcgttt caatctgcgc cacggcggca gcgcgatctc
     4021 gccgcaaacc gcgcacaaat ggttgagcgg gcgcgcaatt ccgagcgacg acaagctcgc
     4081 cacgctggct gcctggttga acgtcaatcc gcactggctg cactacggcc ctccgcccgc
     4141 gaccgtgctg caggcacgcg aacagggcca accgtacgag gtggcggaac cggcggtttc
     4201 gcaggaagca ctggcgctcg cacgcaagat ccagctgctc ggtccgcacc ggcgctatct
     4261 gatcgaggaa ctcgtggaac aactgctgca ggagggcggc tgagaggcgc tgacagaatg
     4321 ccgatggcaa ggcgcgccga ggcaggcagt accgggtagt acggcaggga ggcggaacgc
     4381 cgccagccgc tttttgtcag tgcttctaag gtataatccc cggctctcgc gggtgtagct
     4441 caatggtaga gcagaagctt cccaagctta cgacgagggt tcgattccct tcacccgctc
     4501 cacgtcgact ttccaaggac ttccaccgaa atccaaggag cgttgtaagt cactgatcta
     4561 gctaaggttt ttggccttac ctcggtccag tgacatccag ccaaatccac tgactaccgg
     4621 tacataacgg tacatttagt ggtacatcaa tgcggtgagc gttcgctccg cattgtggct
     4681 ggaggagccg ggccccgata acgaacatgg catccaactc acctttcagg agttggacac
     4741 atgcttacag acatgacggt ccggcaggcc aaggccactg gcaaaccgta caccatcgcc
     4801 gatttcgacg gcctttccct cttcgtttcc gccaccggcg ccaagacttg gcacttccgc
     4861 tatacctgga ttggtcagcg tgcccgtatt tccttaggct gctatcccga gctgtcgctg
     4921 cgcgatgcac gggaactgcg cgacgaggcg cgctccctgg ttgccaaagg catcaatcct
     4981 cgtatcgagc gcaagcaaaa gcgccaagcc atcatgctcg ccggcgagaa caccttcatg
     5041 acggtctacg agcagtggat ggaacaccgc caactcactc ttgaggaagg acgccagagt
     5101 tcgctggaac agatccgccg cgtcttcaag aaggacgtct tcccctacct gaagcggctg
     5161 accatctacg aggtcacccg cccccatctg ctggaagtca tcggccgcat cgagaagcgc
     5221 aactcgctgt cggtagccga gaaggtacgc acctggttca agcagttgtt cgactacgcg
     5281 atggtcgtca tcccggccat ggaaaccaac ccggccaccg acctgcacgt ggtcgcggtg
     5341 ccgctgccgc cggtcgaaca caacccgttc ctgcgcatgg ccgagctccc cgagttcctg
     5401 cagacgctac gcaaatacag cggcatgctg aagacgcagc tggcgatccg cctgctgctc
     5461 ctgaccggcg tgcgaacggg tgagctgcgg ctggccacgc cggatcagtt cgatctggac
     5521 cgtgggttgt ggatcatccc ggtcatgtcg ctcaagcagc gcaagatgct cacccgcaag
     5581 aagcgcaagc gccctaccga catcccgcca tacatcgtcc cgctgtctgt ccaggcgagg
     5641 gagatcgtcc gccacatgct cgacgacttc aaacccgtac agaaatacct gttcgccggg
     5701 gtcaagcgcc tcaccgaccg catgagcgag aacacgctca acgctgcact caagcgcatg
     5761 ggttacgggg accggctgac cggtcacggc attcgcgcga cgatctcaac cgcgctcaac
     5821 gaactgggtt atccgaaagt gtgggtggat gcccagctgt cgcacgccga ccccaaccgc
     5881 atcagcgcca cctataacca cgccgaatat gtcgagcaac gccgggtgat gatgcaggac
     5941 tgggcggacc gcctggatct cttcgaacaa aatcaggtgc agaccgccag catgcacctg
     6001 acgatccatc tgcagggctt gccgacgatc gccgggcagc agaccacccc gctgccgaca
     6061 cctgggcagc acgcatctat cctgctggta gcgcccaccg agcagagcat gccgacgggg
     6121 acacttccga cgcagcgcct gtcggctgtg gagatgcccg agtacgcgca gccaaagctc
     6181 tccgacctgc agcgcgaacg gctgaagctg ctggaaatct ttgaggggcc ggacaacctc
     6241 gtggtggccg actacgccaa gctggccggc aagtcgcgcc gttggatcac ctacgagatt
     6301 caggccggca acctgctgtc gattcagttg gggaacaagg gccaacgtgt tccggtgtgg
     6361 cagctcgatc cgctcaagcg tcaactggtc caggccatcc tcaagcagac gccccgcgga
     6421 gtggatacct gggatatcta tcacgcgctg ctgcgaccgc atgacgccct ggggagccgg
     6481 ccgcccattg aggcagtgac gcccgagaac atgaagatcg ctgtgcgggt ggtcgtggaa
     6541 cagtgttcac agctcgatga accgggggct ccgacgccat acccgatcga ggtgcggcag
     6601 agcgtgcagc gagtgattca aaacgctaac gcggctgatg cggcgctacc tatggggaac
     6661 gattgaaggg catatagccg atcggtagaa ctaccgggaa atacccgggc gatggacacc
     6721 ggagtcagcc tgcttctgtc gccgccacct tcgccgcacg ttcggcaaac ctctcgacag
     6781 ccggcagaca ctctcgcgat ggttgcagta actgggttct gacgacatag gaaccctcgg
     6841 ccagagggcg catggccacg ccccagccac gcacctgtgt gatttgtgag cggggcgcaa
     6901 tgccgacagc gtagcctgcc gccacgagta ccgccaagag gtcaaaggac gtgacctcct
     6961 gggcagaatg cttttccggt ccaaggcgcg catccacctg ctggctcaat gcttcacatg
     7021 cccgcaagca ccattgaagc agcggatagt gcagcaaggc atccaacgga acttcagcgt
     7081 acgcaagcaa cggcgaccgc aatgggaccg ccaaggccag ttcgtctgcc cacagcggct
     7141 gcgctttcat ggcgaagact gttgttgtgg caaccaatgc tatagcggcg tcgtaggcgc
     7201 cgtcctcaag tccgcgggcc aaatccttgg ctgcaacttc ttgcagcagg atgatggtct
     7261 ccggctcctc tgcgcgttgc agggcaagca gactcgcgag ccggggcgac agtgtgtcgg
     7321 ccgaaacggc gagcttcaga atcggtgatc ggtcgggagc actcaatgga atcactccat
     7381 gatggaaatc tggccgcgta ggccccttct catcatagca agagttaaat ttaacgtggt
     7441 taaaagcatg gcggtactcg acgcttctgc caatgcaaaa gcgtactgtc cagcgaggcc
     7501 gccctgtcgg atcgacaacc tatgaacccg aaccggcttt ggcctttggc caagccgttc
     7561 gcgcgactcg gatggaacag ggaatggctc aagaagaact ggcagcccag gcagcaatcg
     7621 agcgctccca tatgggcaag atagagcgcg gagaacacat gcccacgctg gcgctgatcc
     7681 tcagaatcgc catagcactg aacaggagtg ccgcagagct gatcgccgca acagaggaaa
     7741 acctgcgttc gggaccgaga acttagccag cgaaaaggaa gggacattgc ccgtcactcg
     7801 tctatctgct cgtgcaaacg cgctatgaat cgatccagag atatggcagc aggagtgtca
     7861 gtgtcgcttt ccggtcgtaa cagataggtc gtgtgaataa cgtttggagt cgcaagggga
     7921 cgaattacga tgtcgggacg atttaaaacc cctattcgtg cagcgctcgc gaatccaacg
     7981 ccatagcctg ctgcgaccaa cgtcagcatc atgtccaccg aggcaacgta ctcgacatcg
     8041 agcttgaccg ctgcatctcg cagcaattga cccacctcgc aatcccagtt gccgaatagc
     8101 cggcgatcgg ccactacgag cggataacgt atcaagtctt ccaaaggtat ttgcttgtga
     8161 acgagcagcg gatgccgcgc cgccaccgct acgactaccg agtcaaccca gatcggttcg
     8221 gcgatgatgc cgtccccgac atcggcggta tacgcaaacc ccaaattgaa gttccctgaa
     8281 cggagtccgc gcaattgatc ggccagcgag gcctcggtca gacgtatctc gactgccgga
     8341 tcttcctcgc ggcagcgtgc gagaaaggtt gagaggcgcg gaccaatgca tccacggggg
     8401 acggcaatgc gaagaatgcc caggtagcct gaagacactg cctttacatt ttcacgggcc
     8461 agttccagcg tcgaaaacac ccgatgcaca tcctggagga aaacctcgcc agcctgtgtc
     8521 agccgtgtac cgcgccgatc gcgatcaaat agcagcacgt caagttcatc ttcgagttcc
     8581 ttgatggtgc gggacagtgg ggactgctcg atatgcaatc gttcagcggc acgagtgaag
     8641 tgcaactcct ccgcgaggac aacaaagcag cgaagatggc gcaattccaa acctggccct
     8701 cccacgagac gccatttcct ggtaatggca tcgattcttg ccccccgact ttggggacga
     8761 aagattggat tgatgttcgc cgcggcaaca atggcgctcc ataatttcca gcgaacgcgc
     8821 tggcattgag actccctcat gcgccattta gcaactgccc ggttgtgaat gcacacacat
     8881 tgttatttta ccaatttttg aaaatgcctc gccgtcctgt gggtagaagg tctatttcac
     8941 cttaaatcgc aaaaaatcag aaagctgccc cgattccaca atttcgccgt gcggcagcaa
     9001 caggtacagg ggcaaacgaa gtaagaggag tccggcaatg atctcccgtg tatcttccgg
     9061 gtacgtgaca gtcagtcaga agagaagacg ccccttagca gatggtccgg tacccggccg
     9121 agtgaatgaa ccgggaaatt tctacgaagg ctggcacacg gagtcagtgg agggcgatag
     9181 agacaggtaa gggttattcg gcggtaaatc gatgaacaga acacgcggat cttccagcag
     9241 gtagccgtgg agacgccgcg acttccgcgg ccccgtgaca gtgcaggtcc agatgttcag
     9301 accgttctgc tgcttgcggt ggagttgcag cttttcgaac cgtttctgca cccactgcca
     9361 gtcggccatc tggtcctgct tggcaaggag ggcggtctcg ggatgttcct gagcgtagcg
     9421 ctggaatact ccgggactga ccagatagac ggtgtcggca accgtatgca ccagcgcctt
     9481 ggcgtcgtta attatgagcc cccgctcttc gatacgccgg cggagccagg cgacaaaatg
     9541 ttcaccagat ggcgtttcct ccattcctga agtcgttgtg ggagctggcg ccatcgagt
//
LOCUS       DATLGF010000029         6842 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_143354, whole genome shotgun sequence.
ACCESSION   DATLGF010000029 DATLGF010000000
VERSION     DATLGF010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6842
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143354"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2151)
                     /locus_tag="VM406_00900"
     CDS             complement(<1..2151)
                     /locus_tag="VM406_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVL74544.1"
                     /translation="MLISDICIRRPVFATVMSLIIVLIGLVCYDRLSLREYPKIDEPV
                     INVDTRYLGASAEIVESQVTKPLEDSLSGIDGIDTITSISRAEQSQITIRFRLDKDPD
                     VGASDVRDRVSRVRARLPQSIDEPVIAKVEADASPVIWLSFSSETMSQLDLTDIANRI
                     VKPRLQLLPGAADVRIGGERKYAMRIWLDRDRLASYRLTAADIEDALRRQNVEVPAGR
                     IESRQREFSVVSQTDLSTPEEFGAIVIRTVNGYPVRMRDVARVEVGPAAERNSVRFNG
                     QNAVSLGVVRQATANPLELARYVKAEVPRLNEELAPQGVSIAISVDNTVFIERSIQSV
                     YMTIAEAVVLVALVIFVFLRSFRASLIPLVTIPVSLIGAFGLMYLAGFTINTLTLLAL
                     VLAIGLVVDDAIVVLENIYRHVENGMKPVEAAFKGAKEIGFAVVAMTLTLAAVYAPVA
                     FTPGRTGRLFIEFALTLAGAVLVSGFVALTLSPMMCSKLLKHQEKHGVVYRVIENALL
                     ALASGYRRAVAFTLSHRWLVLLAYLLVIGGLVWLGTGMKRELAPTEDRGTIFTALSGP
                     DGASLQYTEKYARQLEQIAAEVPEIDRVFIVAGNPTVERGVAFFRTVDWSERNRSTMD
                     MIREMQPKMAAVPGVLAFPNAPPSLGQSIRERPVSLVVMTNASYGELAEAVQQIIAEA
                     STNPGLTNLDSDLRLNKPQVSVEVDRDRAADAGVQ"
     gene            complement(2162..3328)
                     /locus_tag="VM406_00905"
     CDS             complement(2162..3328)
                     /locus_tag="VM406_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVL74545.1"
                     /translation="MSPRKALYPLVAAVGIGALGYYAYHAQRQPNQPAPAIETARAPA
                     AAQAGGRQGGAGPVAVDVADVRKMSLVDEVHAVGSLRSNESVVIRPEVSGRITRLNFK
                     DGQRVERGQLLVGFDATVNQAEVRQAEAELNIAKANYNRNADLAKQKFISDRARDESM
                     ANVQVLEARLALAQARLSKMEIRAPFSGTVGIRNVSVGDFVKDGADLINLEDISSVKV
                     DFRVPERYVDAARPGQLIEVMVDALPGQVFRARVDAVDPQIDSTGRSALLRGRINNPE
                     GRLKPGMFARVRLILAERAQALVVPEEAIVPQGGEFSVWKVVDGKAVRTPVRTGLRRD
                     AQVEVVEGLTEGDVVVTAGQIRLSRDGTPVRIAGAPPASNAAPIAQPAKPPSRG"
     gene            complement(3415..3855)
                     /gene="rnhA"
                     /locus_tag="VM406_00910"
     CDS             complement(3415..3855)
                     /gene="rnhA"
                     /locus_tag="VM406_00910"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HVL74546.1"
                     /translation="MDKVEIFTDGACKGNPGRGGWGALLIAGGHTKELFGGELNTTNN
                     RMELRAVIEALGALKRPCEVIVHTDSQYVQKGISEWIHNWKARGWKTAAKEPVKNADL
                     WQALDAAQAQHKIQWRWVKGHAGHPGNERADQLANMGVASVAFV"
     gene            complement(3859..4629)
                     /locus_tag="VM406_00915"
     CDS             complement(3859..4629)
                     /locus_tag="VM406_00915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL74547.1"
                     /translation="MDQPSPEKSIIALGPWLDTPAGSYVKAWQQARLDMLTADIFGFN
                     AIQIGLPQLNALQDSRMPNRWLTDTHVPNGDVAGALPVVVAHDFAELPFASQSLDLVV
                     LPHVLEFAQEPHQVLREVERVLIPEGQVIVCGFNPASMWGVRQVASRMTGKPFLPEHG
                     EFISVPRLKDWLKLLNMEVNRGHFGCYAPPCATEKWLQRFRFMERAGGRWWPVLGAVY
                     IVQAIKRVRGMRLIGPAWSKKPATSRRGVPATNRIHKK"
     gene            4633..5406
                     /gene="gloB"
                     /locus_tag="VM406_00920"
     CDS             4633..5406
                     /gene="gloB"
                     /locus_tag="VM406_00920"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HVL74548.1"
                     /translation="MLQVLTVPAFQDNYLWLIHDGEHAAVVDPGDAAPVLEALREHRL
                     SLVAILLTHHHADHVGGVEALLRQFKVPVFGPANDGIAAVTVPLREDDIVDVPQLGLT
                     LRVLEVPGHTLGHIAYHAPQPGWLFCGDTLFAGGCGRLFEGTAEQMAASLGKLALLPD
                     QTQVYCAHEYTIANLRFALEVEPANQALQQRMQQEQAKRAQGRPTVPSTIAVEKATNP
                     FLRYRETAIADRLIQEGHLSSREPIAVFAALRHWKNSYR"
     gene            5548..>6842
                     /locus_tag="VM406_00925"
     CDS             5548..>6842
                     /locus_tag="VM406_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HVL74549.1"
                     /translation="MHSLTRRIIPSLLVLLSGIPASAIATDLTLPYRSWSQLSAKAAA
                     PFDPNDPIGEWLMMEEIDVWGRIRKGFAMPDLDHPLVATHTQWFAARPEYFDRTTPRA
                     ARYLYHVVQELEKRGMPTELALLPFIESAFNPHAYSSAKAAGLWQFIPSTGREYNLTQ
                     SVFMDERRSVIASTEAALTYLQKLHGMFGDWHLALAAYNWGEGSVQRAMKRSRAAGGA
                     TDFNGLARFMPAETRNYVPKLQALKNVIAAPDQYAIALPRVDNQPYFVTIGKTRDIDL
                     SLAAQLAEMPLEEFKALNPQFNKPVIPGGSGTQILLPRSNAEKFKENIANWSRALSSW
                     SAHTVTSTRERLETIAKKFGTTPEVLREVNNIPPRTALAAGSTILVPKSEAAADKNIA
                     PELLETARLVVVPDVETRRTTVKVSRNDTLSAICATLTR"
BASE COUNT         1238 a         2296 c         2101 g         1207 t
ORIGIN      
        1 ctgcacgccg gcatcggccg cgcggtcgcg atcgacttcg acgctgacct gcggcttgtt
       61 caggcgcaga tcggaatcga ggttggtcag tccgggattc gtgctggcct cggcaatgat
      121 ctgttgtacc gcctcggcca gttcaccgta tgaggcgttg gtcatcacaa ccaaactcac
      181 cgggcgctcg cggatcgact gccccagcga cggcggcgcg ttcgggaacg ccagcacgcc
      241 gggcacggcc gccatcttcg gttgcatctc gcgaatcatg tccatcgtgc tgcgattgcg
      301 ctcggaccag tcgacggtac ggaagaaggc gacgccgcgc tcgacggtcg ggttgccggc
      361 aacgatgaac acgcgatcaa tctccggcac ctcggcggcg atctgctcga gctggcgcgc
      421 atatttctcg gtgtactgca gggacgcgcc atccggtccg ctcagcgcgg tgaagatggt
      481 gccgcggtcc tcggttggcg ccagctcccg cttcatgccg gtccccagcc acaccaggcc
      541 gccgatcacg agcagatagg cgagcaagac cagccagcgg tgcgacagcg tgaaagccac
      601 cgcacgacga tatccgctgg cgagcgcgag caatgcattc tcgatcacgc gatagacgac
      661 accgtgcttc tcctggtgct tcaacagctt ggagcacatc atcggcgaca gcgtcaacgc
      721 aacgaagccg gacaccagca cggcgccggc cagcgtcagc gcgaactcga tgaacagccg
      781 cccggtgcgg cccggcgtga acgcgaccgg cgcgtagacc gccgccagcg tgagcgtcat
      841 cgccaccacc gcaaagccga tctccttcgc gcccttgaac gcggcttcaa ccggcttcat
      901 gccgttctcg acgtgacgat aaatgttttc cagcacgacg atcgcgtcgt cgacgaccag
      961 accgatggcg agcaccagcg ccagcaacgt gagcgtattg atcgtgaatc cggccaggta
     1021 catcaggccg aatgcgccga tcagcgagac cgggatcgtc accagcggaa tcagcgaggc
     1081 gcggaacgag cgaaggaata cgaagatcac cagcgcaacc agcaccaccg cctcggcgat
     1141 ggtcatgtag acggactgga tcgagcgctc gatgaatacg gtgttgtcga ccgagatcgc
     1201 aatgctcaca ccctgcggcg caagttcctc gttcaggcgc ggcacttccg ccttcacgta
     1261 gcgcgcgagc tcgagcgggt tcgcggtggc ctggcgcacc acgccgagcg acaccgcgtt
     1321 ctggccgttg aagcgcaccg agttgcgttc ggccgcgggg ccgacctcca cgcgcgccac
     1381 gtcgcgcatg cggaccggat agccgttgac ggtgcggatc acgatcgctc cgaattcttc
     1441 cggcgtggac agatcggtct gagagacgac ggagaactcg cgctggcgcg actcgatgcg
     1501 gccggccggc acttcgacgt tctggcggcg cagcgcgtct tcgatatcgg cggcggtcag
     1561 tcggtacgag gcaagacggt cgcggtccag ccagatgcgc atcgcatact tgcgctcgcc
     1621 gccgatgcgc acatcggccg cgccgggcag cagctgcagg cgcggcttga cgatgcggtt
     1681 ggcgatgtcg gtcaggtcga gctggctcat cgtctcgctg gagaacgaca gccagatcac
     1741 cgggctcgcg tccgcctcga ccttggcgat caccggctcg tcgatcgact gcggcaggcg
     1801 cgctcgcacg cgcgagacgc ggtcacgcac gtcgcttgcg ccgacgtccg gatccttgtc
     1861 gagacgaaat cggatcgtga tctggctttg ctcggcgcgt gaaatcgacg tgatggtatc
     1921 gatgccgtcg atgccggaca gcgaatcctc gagcggcttg gtgacctgcg actcgacgat
     1981 ctcggcactc gcgccgagat agcgggtgtc gacgttgatc accggctcgt cgattttcgg
     2041 atactcgcgc agcgacagcc ggtcgtagca gaccaggccg atcagcacaa tgatcagcga
     2101 catcacggtc gcaaagaccg gacgccggat gcagatgtcg gaaatcagca tgtgcgccct
     2161 ctcagccgcg tgacggcggc ttggccggct gggcgatcgg cgcggcattg ctggccggcg
     2221 gcgcaccggc gatgcgcacc ggggtgccgt cgcgcgacag ccggatctgg ccggcggtga
     2281 cgacgacgtc tccttcagtc aggccttcga cgacttccac ctgcgcgtca cggcgcaatc
     2341 cggtgcgcac cggcgtgcgc acggccttgc cgtcaaccac tttccacacg ctgaactcgc
     2401 cgccctgcgg cacgatcgct tcttccggga ccaccagcgc ctgcgcccgt tcggcgagga
     2461 tcaggcgcac gcgcgcgaac atgccgggct tgagccggcc ttcgggattg ttgatgcgtc
     2521 cgcgcaagag cgccgagcgg ccggtgctgt cgatctgcgg atcgacggcg tcgacacggg
     2581 cgcgaaacac ctgcccaggt agcgcatcga ccatcacctc gatcaactgt ccgggccggg
     2641 ccgcgtcgac atagcgttca ggcacgcgga aatcgacctt gacgctggag atgtcctcga
     2701 gattgatcag gtccgcgcca tccttgacga aatcgccgac gctgacattg cggatgccga
     2761 ccgtgccgga gaatggcgcg cgaatctcca ttttggaaag acgcgcctgc gcgagcgcaa
     2821 gacgcgcttc cagtacctgg acgttcgcca tcgactcgtc gcgggcgcga tcggagatga
     2881 atttctgctt ggcgaggtcg gcgttgcggt tgtagttggc cttcgcgatg ttcagctcgg
     2941 cctcggcctg gcgcacctcg gcctggttta ccgtggcatc gaagcccacc agcagctgcc
     3001 cgcgctccac gcgctggccg tccttgaagt tgagccgggt gatgcgaccg gatacctcgg
     3061 gccgaatcac gacggattcg ttcgaacgca agctgcccac cgcgtgcacc tcgtcgacca
     3121 ggctcatctt gcgcacatcg gccacgtcca ccgcgaccgg acccgcgccg ccctgccgtc
     3181 ctcccgcctg ggccgccgca ggcgctctag cagtctcgat cgcaggcgca ggttgattgg
     3241 gctggcgctg cgcgtggtag gcgtagtaac caagcgcgcc gatgccgacg gcggcgacca
     3301 gcgggtacag ggctttgcgg gggctcatga tcgaaacgta tccggacggc aatgaaatcg
     3361 gcaacgctcg aatttagcac aggcaggaaa accgcgaatg cccgatatac acgcttatac
     3421 aaacgccacc gaagccacgc ccatgttggc cagctggtcc gcgcgctcgt tgccgggatg
     3481 cccggcgtgc cccttcaccc accgccactg gatcttgtgc tgcgcctggg cggcgtcaag
     3541 cgcctgccac aggtctgcat tcttgaccgg ctccttcgcg gccgtcttcc agccgcgcgc
     3601 cttccagtta tggatccatt cgctgatgcc cttctggaca tactggctgt cagtgtggac
     3661 gatgacttcg cacgggcgct tcagcgcgcc gagcgcctcg atcaccgcac gcagctccat
     3721 ccggttgttg gtcgtgttca gttcgccgcc gaacagttct ttcgtatgcc cgcctgctat
     3781 cagcaaggcg ccccatccgc cgcggcccgg attacccttg caggccccgt cggtaaaaat
     3841 ttcgactttg tccatccctt atttcttgtg tatgcgattc gtggccggca ctccgcgacg
     3901 cgaggtcgcc ggcttcttgc tccaggccgg gccgatcagg cgcatgccgc gcacccgctt
     3961 gatcgcctgg acgatataga cagctcccag caccggccac cagcgtcccc ctgcccgctc
     4021 catgaagcgg aaacgctgca gccatttctc cgtcgcgcac ggtggcgcat agcagccgaa
     4081 atgaccgcga ttgacctcca tgttcaacag cttcagccag tccttcaggc gcggcacact
     4141 gatgaactcg ccatgctccg gaagaaacgg cttgccggtc atgcggctgg ccacctggcg
     4201 cactccccac atgctggccg ggttgaagcc gcagacaatc acctgccctt caggaatcaa
     4261 tacgcgttcg acctcgcgca ggacctggtg cggctcctgc gcgaactcga gcacatgcgg
     4321 cagcacgacg agatcgaggc tttgggacgc gaatggcagt tcggcaaaat cgtgcgcgac
     4381 gacgacgggc agcgcaccgg ccacatcgcc attcggcaca tgcgtgtcgg tcagccagcg
     4441 gttcggcatg cggctgtcct gcagcgcgtt cagctgcggc aggccgatct ggatcgcatt
     4501 gaagccgaag atatcggcgg tcagcatgtc caggcgcgcc tgctgccagg ccttcacata
     4561 gcttcccgcc ggcgtatcga gccaaggacc gagggctata atggattttt cgggtgacgg
     4621 ctgatccatc gtatgttgca agtcttgact gtaccggcgt tccaggacaa ttacctctgg
     4681 ctgattcacg atggcgagca cgccgccgtg gtcgatcccg gcgacgcggc cccggttctt
     4741 gaagccttgc gcgaacaccg tctttccctc gtcgctattc tactgacaca tcaccatgct
     4801 gaccatgtgg gcggtgtgga ggcgctcctg cgccagttca aggtcccggt gtttggtccc
     4861 gcgaatgacg gcatcgccgc cgtaaccgtg ccgctgcgcg aagacgatat cgtggatgtg
     4921 ccgcagctgg gcttgacgct tcgggtgctc gaagtgcccg gacatacgct tggtcacatc
     4981 gcctaccacg cgccgcagcc aggttggttg ttctgcggag acacgctgtt cgccggcggc
     5041 tgcggccgtc tgttcgaggg caccgcggaa cagatggcgg cgtcgcttgg caaacttgca
     5101 ctgcttcctg atcaaacgca agtttactgc gcgcacgagt acaccattgc aaaccttcgt
     5161 ttcgctctcg aagtcgaacc cgcgaaccag gccctccagc agcgcatgca gcaagaacaa
     5221 gcaaagcgcg cccaaggcag gcccacggtt ccgtcgacga tcgccgtgga gaaggccacc
     5281 aatccgttcc tgcgatatcg cgagacagcc attgccgatc gcttgattca ggaaggacat
     5341 ctttcctcgc gggaaccaat tgccgttttt gctgccttac ggcattggaa aaattcctat
     5401 cgttgacgag cgtcatcgcg cggcgattgt tgccgcgcaa ctcttgacgc aggaaaaccc
     5461 ggttacgtac acttcgtcgc ttcgcctgaa gcgggcagcc tcgcatgccc gcttcatgcc
     5521 tgtgactctc tcattacgcg aggacccatg cattcgctga caagaagaat aattccttcc
     5581 ctgttggttc ttctgtccgg cattcccgcc tccgccatcg caaccgacct caccctcccg
     5641 taccgcagct ggtcgcaact ctccgccaag gcagccgcgc ccttcgatcc caacgatccc
     5701 atcggcgagt ggctaatgat ggaggagatc gacgtatggg ggcggattcg caagggattt
     5761 gcgatgccgg atctcgacca tccgctggtc gcgacgcaca cacagtggtt cgccgcgcgc
     5821 cccgagtact tcgaccgcac cacgccgcgc gccgcgcgct atctctacca cgtggtgcag
     5881 gaactggaaa agcggggcat gccgaccgaa ctggcgctgc tgccgttcat cgagtccgcg
     5941 ttcaatccgc atgcctactc cagcgccaag gctgccggcc tgtggcagtt catcccctcg
     6001 accgggcgcg agtacaacct cacccaaagc gtgttcatgg acgaacgccg cagcgtgatc
     6061 gcctccaccg aggcggcgct gacctatctg cagaagctgc atggcatgtt cggcgactgg
     6121 catcttgcgc tggcggcata caactggggc gaggggtcgg tgcagcgcgc gatgaagcgt
     6181 tcgcgggccg caggcggcgc caccgacttc aacggtcttg cccgcttcat gccggccgag
     6241 acgcgcaact acgtgcccaa gctgcaggcg ctgaagaacg tgatcgcggc gcctgaccag
     6301 tatgcgatcg ccctgccgcg cgtcgacaac cagccttact tcgtcaccat cggcaagacc
     6361 cgcgacatcg atctctcgct tgccgcccag ctggccgaga tgccgctgga ggagttcaag
     6421 gcgctcaacc cgcagttcaa caagccggtc attcctggcg gatcgggtac gcagatcctg
     6481 ctcccgcgaa gcaatgccga gaaattcaag gaaaacatcg cgaactggag tcgcgcacta
     6541 tcgtcttgga gcgcgcacac cgtgaccagc acacgcgagc ggctggagac catcgcgaag
     6601 aaattcggaa ccacacccga agtgctgcgc gaagtgaaca acatcccgcc gcgcaccgcg
     6661 cttgccgccg gctcgacgat cctcgtcccc aagagcgagg ccgccgcaga caagaacatc
     6721 gcgccggagc tgctcgagac cgcgagactg gtggtcgtgc cggacgtcga aacccggcgc
     6781 acaaccgtca aggtcagtcg caacgatacg ctttccgcga tctgcgcgac gctgacacgg
     6841 tg
//
LOCUS       DATLGF010000030         7127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_17611,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000030 DATLGF010000000
VERSION     DATLGF010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 7127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7127
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17611"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..4890)
                     /locus_tag="VM406_00930"
     CDS             complement(181..4890)
                     /locus_tag="VM406_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HVL74550.1"
                     /translation="MKAKDLRSEALKRAYGTDEAKAPVPALAAAWLNAVEDEDLAGLD
                     AAHLVAPLRDGFPRVDCWRTSDGCRIESFDYEDGHGGTASALLVINPDVPFLVDSIVM
                     ALRRLQMPSRAVLNAVLVTERDASGQIISAAKAGRTRGEHESYVLCLMSEALGEQELE
                     ALKAAIRMAAGDAAVVCRDGPAMQAKMAQVGATAASRDASGQEVADFLDWARNGGFEC
                     FGYARYHIVPGQRDLVREPGSAVGVLADRGHPVYGNCLAGIPGDFDTLSSRRETLSVV
                     KADAHSTLHRDQQLDFIGVRDSDASGAVLGEHCFVGLFSRAAAASPLLQMPFARRRIR
                     QALTLAGVRPEGFRAEKFLEILESLPRTEVLEADPEWLAAVCGAVVALYKQPRGKVFA
                     RRDVYGRHLNVLAYVPRERYSGHLSDHLAQTLRSLSGARDVRVNTLVSDGPLARIYLI
                     AAGADITLDLETAVQQPLLEVVEGWHGALERMVDRTEDRALRNALRKFQSTLPADYVN
                     SVAPEVAYRDLCSLLGAPSAGRVRVRIEAGGDAVTIRLHSVNNVPPLSHILPALHNAG
                     VAIQRERTHAIPAGDGRRYFVTCLTIDTDSAMKLAKPQVAQAAQELFEALFNDEAEDG
                     RMNALVVEAGLRMKEVQLVRAYTSFWRQAGCRFSLRYMAERLKTQPGHVRTLVEAFCA
                     RFDPALSAEQRAFGTATLTGFRQRLSGVDHADTENILAAIADLMLATLRTSYFQPGQG
                     DTIVFKFDSSQLALLPQPRPYREIYVFARRFEGVHLRGGPVARGGLRWSDRMEDYRTE
                     VLGLVKAQMVKNAVIVPAGAKGGFVCKMIPRDATREAVAVEGEAVYRLFISGLLDVTD
                     NRVQGKIVRPAHTVCYDDPDPYLVVAADKGTATFSDIANAIAVRRGFWLGDAFASGGS
                     NGYDHKKLGITAKGAWEAVKRHFYELGHDLDTTPITVVGIGDMSGDVFGNGMLLSRQV
                     KLVAAFDHRHIFVDPNPDTAVSFEERRRLFAQPRSSWDDYDKTLISRGGGVWPRTARA
                     IELSPEARAVLGVDATSMTPDELMHAILKAPVDLFYNGGIGTYIKASTETHEQVKDRA
                     NDAIRVDGNQLRCRVVAEGGNLGATQAGRIEYALAGGHIFTDAIDNSAGVDCSDHEVN
                     LKIWLDTEVNAGKLAAEERNRLLNQVTPDVVALVLRDNALQTRLLVRETQAQSEPAVR
                     EAYAALIARLEGEGAFSRKLEQLPDAAVLAARGEQGKGLSAPELAVVIAHVKNRYKRL
                     LAEQNLLQHPWARSLLTPYFPPALTEGRDALEHPLANAILATVLANEAVNRCGPLMLA
                     EIALQRAVRETDVIGAWAQGWAALNLAPLFSALDAHALEVPRAVSQQVDRRSRALLRS
                     AIHGVLSVPEQQLRASGAIAELTALFGQPEALRELAPGAAERPSAAHPSLPSAFAQAW
                     DAIEAIAAIADFLFAGLAVPRPQGMDLPTFLRLGLTLRREAGIDGLERVLTHASASEA
                     QAPLRDHALQALRRAQQRLLMRVLHEAPNGGDPALLVTALIDKLRVGARNAAPSDLEH
                     AVLDVWALSEAASGEEA"
     gene            complement(5128..6471)
                     /gene="astB"
                     /locus_tag="VM406_00935"
     CDS             complement(5128..6471)
                     /gene="astB"
                     /locus_tag="VM406_00935"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HVL74551.1"
                     /translation="MMAREFNFDGLVGPTHNYAGLSFGNIASHSNINAVSNPREAALQ
                     GLAKMRTLARRGFAQALLPPQLRPNFDLLRRTGFTGSDAEVLARAQKEAPSILACAWS
                     AAPMWTANAATVSPSADTADGRVHFTVANLNNKLHRSYEHLQTARTLRAIFADARHFA
                     VHDALPQTGAFGDEGAANHTRLCAAHGEPGVEMFVYGRTEFDPGAPQPRRFPARQTLE
                     ASAAVARLHGLDPGRTLFVQQEPDTIDAGVFHNDVIAVGNANVLFYHQHAFAEEGRVL
                     DGLRRMLGRLGAELVPVRVSAADVPLDDAVASYLFNSQLLSRPDGRMALVVPQECEEV
                     PSVARYLKRLVAGSGPIDELISFDLRQSMRNGGGPACLRLRVALTDAEAAAMHQGVVL
                     TEDLYGRLVAWVGRHYRDRLAPQDLADPQLVSEVRAALAELEQILALPGLFAELE"
     gene            complement(6471..>7127)
                     /locus_tag="VM406_00940"
     CDS             complement(6471..>7127)
                     /locus_tag="VM406_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVL74552.1"
                     /translation="AFISAGQRCTCARRLIVEDGPRGEALLARLVEVAARIEVGQYDR
                     DPPPFMGPLVSAAGAEAMLRTQADLVARGGRLLLEMHRLQPGTGFVSPGIVDITNARD
                     VPDEEWFGPLLQVVRVPDFDAAMTAANATAYGLAAGLLSDDSALWERFQVEVRAGVVN
                     WNRPTTGASSSLPFGGVGKSGNHRPSAYYAADYCAWPVASLQSEALQMPRQPTPGLVF
                     "
BASE COUNT         1144 a         2494 c         2397 g         1092 t
ORIGIN      
        1 ccatgcacac caacggacag gagtacacgc acccggtccc caccgccccg gcacaggctc
       61 agcgtgcccg gggccgcctc gccgcacacc catccggcgc gcgcaaaccg gccggaccag
      121 cgcccgagat ctcccgctgc cagtgcggcg accgagggcg acagcttcat cggcccgccc
      181 tcatgcctcc tcgcccgatg ccgcctccga cagcgcccac acgtcgagca cggcatgctc
      241 caggtcggac ggcgcggcat tgcgcgcacc cacgcgcagc ttgtcgatca gcgccgtcac
      301 caagagcgcc ggatcgccgc cattcggcgc ctcgtgcagc acccgcatca gcaggcgttg
      361 ctgcgcgcgc cgcagcgcct gcagcgcgtg gtcgcgcagc ggcgcctgcg cctcgctggc
      421 gctggcatgg gtgagcaccc gctccagccc gtcgatgccg gcctcgcggc gcagcgtgag
      481 ccccagcctg aggaaggtcg gcagatccat gccctgcggg cgcggaacgg cgaggcccgc
      541 gaacaggaaa tcggcgatcg cggcgatcgc ctcgatcgca tcccaggcct gggcgaacgc
      601 tgacggcaga gacgggtggg ccgccgaagg acgctcggcc gcgcccggcg caagctcgcg
      661 cagcgcctca ggctgaccaa acagcgcggt cagttccgca atcgcgccgg atgcgcgcaa
      721 ctgctgctcc ggcaccgaga gcacgccgtg gattgcggac cggagcaatg cacgcgaacg
      781 gcggtccacc tgctgcgaca ccgcgcgcgg cacctccagc gcatgcgcat cgagggcgga
      841 gaacagcggg gcgagattca gcgccgccca gccctgcgcc caggcgccga tcacatcggt
      901 ttcgcgcacc gcgcgctgca gggcgatctc cgcaagcatc agcggcccgc agcggttgac
      961 cgcctcgttg gccagcacgg tggcgaggat cgcgttggcg agcggatgct cgagcgcatc
     1021 gcgcccttcg gtcagcgccg gcgggaaata gggcgtgagc aggctgcgcg cccatggatg
     1081 ctgcagcagg ttctgctcgg ccagcaggcg cttgtagcgg ttcttcacat gcgcgatcac
     1141 aaccgccagt tccggcgcgc tcaggccctt gccctgttcg ccgcgcgcag caagcacggc
     1201 ggcatccggc agctgctcga gcttgcgcga gaacgcgccc tcgccttcga gccgcgcgat
     1261 cagggccgcg taggcctcgc gcaccgccgg ttcggattgg gcctgggttt cacgcaccag
     1321 cagccgcgtc tgcagcgcgt tgtcgcgcaa taccagcgcg acgacgtccg gggtgacctg
     1381 gttcagcagc cggttgcgct cctcggccgc caacttgccg gcgttgactt cagtgtccag
     1441 ccagatcttc aggttgacct catggtccga gcagtcgacg ccggcggagt tgtcgattgc
     1501 gtcggtgaag atgtggccgc cggccagcgc gtattcgata cgcccggcct gggtcgcgcc
     1561 gaggttcccg ccttcggcga ccacgcggca gcgcagctga ttgccgtcga cgcgaatcgc
     1621 gtcgttggcg cggtccttca cctgctcgtg ggtctcggtc gaggccttga tgtaggtgcc
     1681 gatgccgccg ttgtagaaca ggtcgaccgg cgccttcagg atcgcgtgca tcagctcgtc
     1741 cggtgtcatg ctggttgcat ccacgccgag caccgcccgc gcttccggcg acagctcgat
     1801 cgcgcgcgcg gtgcgcggcc acacgccgcc gcccctggaa atcagggtct tgtcgtagtc
     1861 gtcccaggac gaacgcggct gggcgaacag gcgccggcgt tcctcgaagg acacggcggt
     1921 atccgggttc gggtcgacaa agatgtggcg atgatcgaag gccgccacca gcttcacctg
     1981 gcgcgacagc aacatgccgt tgccgaacac gtcgcccgac atgtcgccga tgccgaccac
     2041 ggtgatcggc gtggtgtcga gatcgtggcc gagttcgtag aaatgccgct tgaccgcttc
     2101 ccaagcgccc ttcgcggtga tgccgagctt cttgtggtcg tagccgttcg agccgcctga
     2161 cgcgaacgcg tcgccgagcc agaagccgcg ccgcaccgcg atcgcattgg cgatgtccga
     2221 aaaagttgcc gtgcccttgt ccgcggcgac caccagatac ggatcgggat cgtcgtagca
     2281 gaccgtgtgc gccggacgta cgatctttcc ctgcacgcgg ttgtcggtca cgtcgagcag
     2341 gccggaaatg aacaggcgat acacggcctc gccttcgacc gccaccgctt cacgcgtcgc
     2401 atccctgggg atcatcttgc agacgaaacc gcccttggcg ccggccggca cgatgaccgc
     2461 gttcttcacc atctgcgcct tcaccagccc gagtacttcg gtacggtagt cctccatccg
     2521 gtcggaccag cgcaggccgc cgcgtgcgac cggaccgccg cgcaggtgca cgccctcgaa
     2581 gcggcgcgcg aatacgtaga tctcgcgata aggccgcggc tgcggcagca gcgccagctg
     2641 gctgctgtcg aacttgaaga cgatggtgtc gccctgcccg ggctggaagt aggaggtgcg
     2701 cagcgtcgcc agcatcaggt cggcgatcgc ggcaagaatg ttctcggtgt ccgcgtgatc
     2761 gacgccggac aggcgctggc ggaaaccggt cagcgtggcg gtgccgaatg cgcgctgctc
     2821 ggccgacagc gccggatcga atcgcgcgca gaaggcctcg accagcgtgc gcacgtgtcc
     2881 gggctgggtc ttgagccgct cggccatgta gcgcagcgaa aagcggcagc cggcctggcg
     2941 ccagaagctc gtgtaggcgc gcaccagttg cacttccttc atgcgcaagc cggcttcgac
     3001 caccagcgcg ttcatccgcc catcctcggc ctcgtcgttg aacagcgcct cgaacagttc
     3061 ctgggccgcc tgcgcgacct gcggcttggc gagcttcatc gcgctgtcgg tgtcgatggt
     3121 gaggcaggtc acgaagtagc ggcggccgtc gccggccgga atcgcgtggg tgcgctcgcg
     3181 ctggatcgcg acgccggcgt tgtgcagcgc cggcaggata tgcgacagcg gcggcacgtt
     3241 gttgaccgaa tgcaaacgta tcgtcacggc atcgcccccg gcttcgatgc gcacccgcac
     3301 ccggcccgcg gaaggcgcgc cgagcagcga gcacaggtcg cggtaggcga cttcgggagc
     3361 gaccgagttc acatagtcgg ccggcagcgt gctctggaac ttgcgcagcg cgttgcgcaa
     3421 tgcccggtcc tcggtgcggt cgaccatgcg ctccagcgcc ccgtgccagc cttccaccac
     3481 ctcgagcagc ggctgctgca cggcggtttc caggtccagc gtgatgtccg cgccggcggc
     3541 gatcaggtag atgcgcgcca gcggaccgtc ggagaccagg gtgttgaccc gcacatcgcg
     3601 cgcaccggac aggctgcgca gcgtctgcgc aagatgatcg gacaaatggc cgctgtagcg
     3661 ctcgcgcggc acgtaggcga gcacgttcag gtggcggccg tacacatcgc ggcgcgcgaa
     3721 caccttgcca cgcggctgct tgtacagcgc gacgacggcg ccgcacactg cggccagcca
     3781 ttcgggatcg gcttcgagca cctcggtgcg cggcagcgat tccaggatct ccagaaactt
     3841 ttccgcgcga aagccctccg ggcgcacgcc ggccagcgtc agcgcctggc gtatgcggcg
     3901 gcgcgcgaac ggcatctgca gcaaggggct ggccgcggcg gcgcgcgaga acaggccgac
     3961 gaagcagtgt tctccgagca cggcgccgct ggcatcgctg tcgcgcacgc cgatgaagtc
     4021 cagctgctgg tcgcggtgca gcgtcgaatg cgcatccgcc ttcacgaccg acaaggtttc
     4081 gcgacgcgag gacagcgtgt cgaaatcgcc cggtatgccg gcaaggcagt tgccatagac
     4141 aggatggcca cgatcggcca gcacgccgac cgcgctgccc ggctcgcgca caaggtcgcg
     4201 ctggccggga acgatgtggt aacgcgcata accgaagcac tcgaagccgc cgttgcgcgc
     4261 ccagtcgagg aaatccgcca cttcctggcc agaagcgtca cgcgaggcgg ccgtcgcgcc
     4321 cacctgggcc atctttgcct gcatcgcggg gccgtcgcgg catacgacgg cggcgtcgcc
     4381 tgcagccatg cggatggccg ccttcaaggc ctccagttcc tgctcgccga gcgcttcgga
     4441 catcaggcac agcacgtagg attcgtgctc gccgcgcgtc ctgcccgcct tggccgcgct
     4501 gatgatctgg ccgctggcgt cgcgctcggt cacgagcacc gcattgagca ccgcgcgcga
     4561 aggcatctgc agacggcgca atgccatgac gatcgagtcg accaggaaag gcacgtcggg
     4621 attgatcacc agcagcgcgc tggccgttcc gccgtggcca tcctcgtagt cgaagctttc
     4681 gatcctgcaa ccgtccgacg tgcgccagca gtccacgcgt gggaaaccat cgcgcaacgg
     4741 cgcaaccaga tgggctgcat cgagcccggc aaggtcctcg tcctctaccg cgttcagcca
     4801 cgccgctgcc agcgcgggta ccggcgcctt cgcctcatcc gtgccatagg cgcgctttag
     4861 tgcttcgctg cgcaaatcct tcgctttcat cgtcatctcc tgttctcact tgtggtgggt
     4921 gtcgcgcgat tcgacgccgc acgcgggtaa tcgtgcggcg tgacgggcag caggcaggtc
     4981 gggccgcggc atggtgggcc agccggtcgc gtgccggcga cgtcgtccac ccgcgcgcat
     5041 cctcctcgcc atgtcgctca cgcgcaggat cgagcgtacc gcctcgacca ccgttgaaaa
     5101 ttttcttatt tccgacgccg cagaacctta ttcaagctca gcgaacaagc ccggcagcgc
     5161 gaggatctgc tccagttccg ccagcgcggc gcgcacttcc gagacgagtt gaggatccgc
     5221 aagatcctgc ggcgccaggc ggtcgcggta atggcgtccg acccacgcga ccaggcggcc
     5281 gtacaaatcc tccgtcagca ccacgccctg gtgcatcgcc gccgcctctg catcggtcag
     5341 ggccacgcgc aggcgcaagc aggccgggcc gccgccattg cgcatgctct ggcgcagatc
     5401 gaagctgatc agctcgtcga tcggcccgct tcccgcaacc aggcgtttca ggtaacgcgc
     5461 aaccgacggc acttcctcgc attcctgcgg cacgaccagc gccatgcgcc catccggccg
     5521 cgagagcaac tggctgttga acagatagct ggcgaccgcg tcatcgagcg gcacgtcggc
     5581 ggcactgacg cgtaccggca caagctcggc accgaggcgg ccgagcatgc ggcgcaggcc
     5641 gtcgagcacg cgaccttctt cggcaaacgc atgctggtga tagaacagca cattggcatt
     5701 tccgaccgcg atcacgtcgt tgtggaagac gccggcgtcg atggtgtccg gctcctgctg
     5761 cacgaacagc gtgcggccgg ggtcgaggcc atgcagccgg gcgactgccg cgctcgcttc
     5821 cagcgtctgg cgcgcgggaa agcgcctcgg ctgcggcgcg cccggatcga actcggtgcg
     5881 gccatagacg aacatctcga cgccgggctc gccgtgcgcc gcgcacagcc gggtgtggtt
     5941 ggctgcgcct tcatcgccga acgcaccggt ctgcggcaat gcgtcgtgca ccgcgaagtg
     6001 gcgggcatcg gcgaagatcg cgcgcagcgt gcgcgcggtc tgcagatgct cgtacgaacg
     6061 atgcagcttg ttgttcagat tggcgacagt gaagtgaacc cggccgtcgg ccgtgtccgc
     6121 gctcgggctg accgtggccg cattggccgt ccacatcggc gcggcggacc aggcgcaggc
     6181 cagaatggac ggcgcttcct tctgcgcgcg cgccagcacc tcggcatcgc tgccggtgaa
     6241 tccggtcctt cgcaacaaat cgaaattggg gcgcaattgc ggcggcagca aggcctgggc
     6301 aaacccgcgc cgggcgagcg tgcgcatctt cgccagaccc tgcagcgccg cctcgcgcgg
     6361 gttggatacg gcgttgatgt tgctgtggga agcgatgttc ccgaacgaca ggccggcgta
     6421 attgtgggtg ggccccacca gcccgtcgaa attgaattca cgtgccatga tcagaacacc
     6481 agtccgggcg tgggttggcg cggcatctgc aaagcctcgc tctgcaggct tgcgaccggc
     6541 cacgcgcagt agtcggccgc gtaataggcg ctcggacggt gattgccgga cttgccgaca
     6601 ccgccgaaag gcaggctgct ggacgcgccg gtggtcggtc ggttccagtt caccacgccg
     6661 gcgcgcacct cgacttggaa acgctcccac agtgcggagt cgtcgctgag caggccggcg
     6721 gcaaggccgt aggcggtggc gttggccgct gtcatcgcgg catcgaaatc gggcacgcgc
     6781 acaacctgca gcagcgggcc gaaccactcc tcgtccggta catcccgcgc gttggtgata
     6841 tcgacgatgc cgggagacac gaagccggta ccgggctgca gcctgtgcat ctccagcagc
     6901 aagcgtccgc cgcgcgcgac aaggtccgcc tgggttcgca gcatcgcctc ggcaccggcc
     6961 gccgacacca gcggccccat gaacggcggc gggtcgcggt cgtactggcc gacctcgatg
     7021 cgcgctgcga cctcgaccag acgtgcgagc aaggcttccc cgcgcggtcc gtcctcgacg
     7081 atcaggcggc gcgcgcaggt gcagcgctgg ccggcgctga tgaaggc
//
LOCUS       DATLGF010000031         7177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_80803,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000031 DATLGF010000000
VERSION     DATLGF010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 7177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7177
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80803"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VM406_00945"
     CDS             complement(<1..339)
                     /locus_tag="VM406_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74553.1"
                     /translation="MMGRTHFIAALAGLYALLAGIIALPCLAVWADLEAGERDALAAM
                     LAERAGLIAFLLLIAMAALGIIFDALYRRHRDQQQERDEPGPFGQHRGQRVALAGFQV
                     CPDGKAGQGDD"
     gene            complement(336..710)
                     /locus_tag="VM406_00950"
     CDS             complement(336..710)
                     /locus_tag="VM406_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL74554.1"
                     /translation="MQKKILIADDEPNIVISLEYLMRREGFEVAVATDGEAALQAMAA
                     RPPDLILLDIMLPKKDGFEVCQQIRANPDWQQVKVVMLTAKGRESEVAKGMALGADAY
                     MTKPFSTRELVAQVKQLLALPR"
     gene            complement(717..3503)
                     /locus_tag="VM406_00955"
     CDS             complement(717..3503)
                     /locus_tag="VM406_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HVL74555.1"
                     /translation="MLQGWVIVLVSFSYLGLLFAIAHYGDRRADAGRSVIDNPTIYAL
                     SLAVYCTTWTFYGSVGRAAATGIGFLPIYLGPTLMVALWWFVMRKIIRISKTNRITSI
                     ADFVASRYGKSQLLGGLVTIIAVIGIIPYISLQLKAVSGSFSILLHYPDIVMPDATGA
                     QPLFQDTALYIAIILAAFTILFGTRHLDATERHEGMVAAIAFESLVKLVAFFAVGLFV
                     TFGIYNGFGDIFSRAREIPELLTLMTVSENGIGYGSWWTLTLLSMLSIMFLPRQFQIT
                     VVENVNEEHLARAIWMLPLYLFLINIFVLPIALGGLMHFGSGTVDADTFVLTLPMAEQ
                     RQALALLVYIGGLSAATGMVIVETIALSTMICNDLVMPVLLRQKWLRLTESADLSGLL
                     LSIRRWAIAAILMLGYLYFRLAGEAYALVAIGLISFAAVAQFAPAIFGGIYWKQGTRN
                     GAIAGLAAGFVVWIYTLLLPSFAKSGWLPGHFLTDGLFGIASLRPLSLFGLTGLDELS
                     HSLFWSLLANIGCYVGVSLAGRPSAAETGQATLFVDVFKRTRGAEGSRVWRGSARVDE
                     LLPLIGRFIGPERARDAFLAYARSRGLSSIADLRADAELVHYAETLLAGAIGAASARV
                     MVASVAQEEPLGLDEVMNILDEASQVRAYSVQLEEKSRALEAASAELRAANERLKELD
                     RMKDDFMSTVTHELRTPLTSIRAFSELLLDDPRMDLSERERFLGIISKETERLTRLIN
                     QMLDMAKIESGNAEWHTEEVDMRAVVRESVDATAQLYQESSVTLDADLPESVPVVLAD
                     RDRLIQVMLNLLSNAVKFCEPGRGQVAVKLRNEQDCLRVDVRDNGPGVSAEDQAVIFE
                     RFRQVSNPHTGKPRGTGLGLPISRQIVERFGGRLWVESVPGRGACFSFTIPLHGRQHD
                     RQSAEPELEAGKTR"
     gene            complement(3508..3708)
                     /locus_tag="VM406_00960"
     CDS             complement(3508..3708)
                     /locus_tag="VM406_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74556.1"
                     /translation="MDRSSITGQRLVAVFLLGCVLLNYPILFLFNKAEGLLGIPSLYL
                     YLFGAWAFLIALMAWLIERNGD"
     gene            4052..6907
                     /locus_tag="VM406_00965"
     CDS             4052..6907
                     /locus_tag="VM406_00965"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HVL74557.1"
                     /translation="MMQQYFSNSYLFGGNAPYVEELYEAYLNNPGSVPDKWRAYFDAM
                     QNVPAVDGSNKPDIAHAPVIASFAERAKQGPIRTVTASADIEMGRKRVAVTQLIAAYR
                     FLGNRWANLDPLQRQERPAIPELEPSFYGFSDADMDIVFNISNTYFGPETATLRDLLQ
                     ALRDTYCRSIGAEFQYISDPGQKRWLQERLESVRSTPNFSPEKKKHILDRLTAAEGLE
                     RYLHTKYVGQKRFSLEGGESFIASLDEAIQRAGSKGVQEIVIGMAHRGRLNVLVNTLG
                     KMPKDLFAEFEGRHADDLPAGDVKYHQGFSSDISTAGGPVHLSLAFNPSHLEIVNPVV
                     EGSVKARMDRRGDKDGSQVLPILVHGDAAFAGQGVVMETLNLAQTRGYGTGGTVHIVI
                     NNQIGFTTSDPRDTRSTIYCSDVVKMIEAPVLHVNGDDPEAVVFATQIALDFRMEFKK
                     DVVVDIVCFRKLGHNEQDTPALTQPLMYKKIAQHPGTRKLYADKLITQSTIPADAGDG
                     MVKAYREAMDAGRHTVDPVISNFKSKYAVDWMPFLNRKWTDAADTAVPLAELKRLATR
                     ITTVPDDFKVHPLVEKVLADRAAMGRGEMNLDWGMGEHLAYASLVASGYAVRLTGQDA
                     GRGTFTHRHAVLHDQKRERWDQGTYVPLQNVSENQAPFTVIDSVLSEEAVLGFEYGYS
                     TAEPNALTIWEAQFGDFANGAQVVIDQFISSGEVKWGRASGLVMMLPHGYEGQGPEHS
                     SARIERFLQLCADNNMQVVQPTTAAQIFHLLRRQMIRLFRKPLVIMTPKSLLRNKDAG
                     SPLSELAKGTFQTVIGEVDDKIDPKKVKRVLACSGKVYYDLINARKERGASDVAIIRI
                     EQLYPFPHKAFAAELKKFPNFTELVWTQDEPQNQGPWFQIQHNIFENLEDGQKLAYAG
                     RPASASPAVGYYDKHYAQQKALIETAFSKLKGFVLTK"
     gene            6968..>7177
                     /locus_tag="VM406_00970"
     CDS             6968..>7177
                     /locus_tag="VM406_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HVL74558.1"
                     /translation="MAIIEVKVPQLSESVAEATLLSWHKKVGEAVGRDENLIDIETDK
                     VVLELPAPDGGVLVEILKEDGATVVA"
BASE COUNT         1394 a         2314 c         2283 g         1186 t
ORIGIN      
        1 atcatcgccc tgccctgcct tgccgtctgg gcagacctgg aagccggcga gcgcgacgcg
       61 ctggccgcga tgctggccga acgggccggg ctcatcgcgt tcctgttgct gatcgcgatg
      121 gcgacggtag agcgcgtcga agattatccc cagcgccgcc atcgcgatca gcaacaggaa
      181 cgcgatgagc ccggcccgtt cggccagcat cgcggccagc gcgtcgcgct cgccggcttc
      241 caggtctgcc cagacggcaa ggcagggcag ggcgatgatg cccgctagca gcgcatacag
      301 gccggcgagg gcagcaatga agtgtgtccg tcccatcatc tgggcaaggc aagcagctgc
      361 ttgacctgcg cgaccagttc gcgtgtcgaa aagggcttgg tcatgtaggc gtcggcgccg
      421 agggccattc ccttggcgac ctcgctctcg cgtcccttgg cggtgagcat gacgaccttg
      481 acctgctgcc agtcgggatt ggcgcggatc tgctggcaca cctcgaagcc gtccttcttc
      541 ggcaacatga tgtcgagcag gatcaggtcg ggagggcgtg cggccatcgc ttgcagcgca
      601 gcttcgccat cggtggcgac cgccacctcg aacccttcgc gcctcatcag gtattcgagc
      661 gaaatgacga tgttcggctc gtcgtccgca atcagtattt tcttctgcat gtctctctac
      721 cgcgtcttcc cggcttccag ttcaggttcg gccgattgcc tgtcgtgctg tcggccgtgc
      781 agcgggatgg tgaatgaaaa gcaggcgccc cggccaggca cgctttccac ccacagccgg
      841 ccgccgaaac gttcgacgat ctgccggctg atcggcaggc ccagtccggt cccgcgcggc
      901 ttgccggtgt gggggttgga aacctggcgg aatctctcga agatgacggc ctggtcctcg
      961 gcagacacgc ccggcccgtt gtcgcgcacg tcgacccgca ggcaatcctg ctcattgcgc
     1021 agcttcaccg caacctggcc ccggcccggc tcgcagaact ttaccgcgtt cgacagcagg
     1081 ttcaacatga cctggatcag gcggtcgcgg tcggccagga cgaccggaac gctctcgggc
     1141 aggtccgcgt cgagcgtgac cgacgattcc tggtagagct gggcggtggc gtcgaccgac
     1201 tcgcgcacca cggcgcgcat gtccacttcc tcggtatgcc actcggcgtt gcccgattcg
     1261 atcttggcca tgtcgagcat ctgattgatc aggcgggtca gccgctcggt ttccttggaa
     1321 atgatgccca ggaagcgctc gcgctcggac agatccatgc gcggatcgtc gagcagcagt
     1381 tcggaaaacg cgcgaatcga agtcagcggc gtgcgcagtt cgtgcgtcac cgtggacatg
     1441 aaatcgtcct tcatccggtc cagttccttg agccgctcgt tcgcggcgcg caactcggcc
     1501 gacgccgctt ccagcgcgcg cgatttttcc tccagttgca cggaataggc ccgcacctgc
     1561 gaagcttcgt cgaggatgtt catcacttcg tcgaggccga gcggctcctc ctgcgcgacc
     1621 gaggccacca tgacccgcgc cgaggcggcg ccgatcgccc cggccagcag cgtttcagcg
     1681 taatgcacca gctccgcgtc cgcgcgcagg tcggcgatgg acgagagccc gcgcgagcgg
     1741 gcataggcga ggaaggcgtc gcgcgcccgc tccgggccga tgaatcggcc gatcagcggc
     1801 agcagctcgt ccacccgcgc gctgccacgc cagacgcgcg aaccctcggc gccgcgcgtg
     1861 cgcttgaaca cgtcgacgaa cagcgtggcc tgaccggtct cggcggcgct cggccggcct
     1921 gcgagcgaca cgccgacata acagccgata ttggccagga ggctccaaaa gaggctgtgc
     1981 gacagctcgt cgagtccggt caggccgaac aggctgagcg ggcgcagcga tgcgatgccg
     2041 aacaggccgt cggtgaggaa gtgcccgggc agccagcccg acttcgcgaa cgagggcagc
     2101 agcagcgtgt agatccagac cacgaagccc gcggcgagcc cggcgatggc accgttgcgg
     2161 gtgccctgct tccagtagat accgccgaag atcgcgggcg cgaactgggc cacggccgcg
     2221 aacgagatca gtccgatcgc gaccagcgcg tacgcttcgc ccgcaagccg gaagtacagg
     2281 tagccgagca tcaggatcgc cgcgattgcc cagcggcgga tcgacagcag caggcccgaa
     2341 agatccgcgc tttcggtcag gcgcagccat ttctggcgca gcaggaccgg catcacaaga
     2401 tcgttgcaga tcatcgtcga cagcgcaatc gtctcgacga tcaccatgcc ggtcgcggca
     2461 gacaatccgc cgatatagac cagaagggcg agcgcctggc gctgctcggc catcggcagg
     2521 gtgagcacga aggtgtcggc atccacggtg cccgagccga aatgcatcag gccgcccagt
     2581 gcgatcggca gcacgaagat gttgatcagg aacaggtaga gcgggagcat ccagatcgcc
     2641 cgcgcgaggt gttcctcgtt gacgttctcc accacggtga tctggaactg gcgcggcagg
     2701 aacatgatgg agagcatcga cagcagggtc agcgtccacc agctgccgta gccgatgccg
     2761 ttttccgaca ccgtcatcag ggtcagcagt tccgggatct cgcgcgcgcg gctgaagatg
     2821 tcgccgaagc cgttgtagat gccgaaggtg acgaacaggc cgaccgcgaa gaaggcgacc
     2881 agcttgacca gcgattcgaa ggcgattgcc gccaccatcc cttcgtggcg ctcggtcgca
     2941 tccaggtgtc gggtgccgaa caggatcgtg aaggccgcga ggatgatcgc gatatacagt
     3001 gcggtgtcct ggaacagcgg ctgcgcaccg gtcgcgtcgg gcatcacgat gtccgggtag
     3061 tgaagcagga tagagaagct gcccgagacg gccttcaact gcagcgagat gtagggaatg
     3121 atcccgatca ccgcgatgat cgtcaccagg ccgcccagga gctggctctt gccgtagcgc
     3181 gaggcgacga agtcggcgat cgaggtgatc cggttggtct tgctgatgcg gatgatcttg
     3241 cgcatcacga accaccagag cgcgaccatc agggtcgggc ccaggtagat cggcaggaat
     3301 ccgatgccgg tggcggcggc gcggccgacg ctgccgtaga aggtccaggt cgtgcagtag
     3361 acggccaggg ataacgcgta gatggtcggg ttgtcgatga ccgagcggcc ggcatcggcg
     3421 cgccggtcgc cgtagtgggc gatcgcgaac agcagtccca ggtaggaaaa cgagaccagt
     3481 acgatcaccc agccctgcag catcgcgtca gtctccgttg cgctcgatca gccaggccat
     3541 cagggcgatc aggaaggccc aggcgccaaa caggtaaagg tagagcgacg ggatgccgag
     3601 caggccttcc gccttgttga acaggaacag gatggggtag ttgagcagca cgcagccgag
     3661 caggaacacg gcgaccaggc gctggccggt aatgctggat cgatccatga tgcctcctgc
     3721 gtcagcatag ttgcgcttac tgacgccacg ctgacggctg cccggaccgg tggggagagg
     3781 gagctgcgat ggcgcgccgc acgggtgttt ttgtcggcta tgcgcaacat cttactaaat
     3841 ccgcaagagg ctgtcgttcc cttcagtttt cgggccgtcg cggccgccgg agcatactgc
     3901 aagtatgcta ttctcttgct tggtaacact ctcaggtcct cggtccttcc cccacaactt
     3961 gatatgtgcg atccgctaac cggtcaggcc gtgtcgcgga aggtttagtc aacccgctaa
     4021 tctcgcgaag cgcgaagaaa ggtgagcaaa gatgatgcag cagtacttct ccaattccta
     4081 tttgtttgga ggaaacgcac cgtacgtcga agaactgtac gaggcgtatc tgaacaatcc
     4141 cggttccgtc cccgacaagt ggcgcgcgta tttcgacgcg atgcagaacg tgccggccgt
     4201 cgacggctcc aataaacccg acatcgcaca cgccccggtc atcgcctcgt tcgccgagcg
     4261 cgccaagcag ggcccgattc gcaccgtcac cgcgtcggcc gacatcgaga tgggacgcaa
     4321 gcgcgtcgcc gtcacccagc tgatcgccgc ctaccgcttc cttggcaacc gctgggccaa
     4381 cctcgatccg ctgcagcgcc aggagcgccc ggcgatcccc gagctggaac cgagcttcta
     4441 cggtttctcg gacgccgaca tggacatcgt gttcaacata agcaacacgt acttcggccc
     4501 cgaaaccgcg accctgcgcg atctgctgca agcactgcgc gacacctact gccgttcgat
     4561 cggcgccgaa ttccagtaca tcagcgaccc cggccagaag cgctggctgc aggagcgcct
     4621 ggagtcggtc cgttcgaccc cgaatttctc gcccgagaag aagaaacaca ttcttgaccg
     4681 cctgaccgcg gccgaaggtc tcgagcgcta cctgcacacc aagtacgtcg gccagaagcg
     4741 cttctcgctg gaaggcggcg aaagtttcat cgcgtcgctg gacgaggcga tccagcgtgc
     4801 cggcagcaag ggcgtccagg aaatcgtgat cggcatggcc caccgcggcc gcctgaacgt
     4861 gctggtcaat acccttggca agatgcccaa ggacctgttc gccgaattcg aaggccgcca
     4921 cgccgacgac ctgccggccg gcgacgtcaa gtaccaccag ggcttctcgt ccgacatttc
     4981 caccgccggc gggccggtcc acctgtcgct ggccttcaac ccctcgcatc tggaaatcgt
     5041 caatccggtg gtcgaaggct cggtcaaggc gcgcatggat cgccgcggcg acaaggacgg
     5101 ctcgcaagtg ctgccgatcc tggtgcacgg tgacgccgcc ttcgccggtc agggcgtggt
     5161 gatggaaacc ctgaacctgg cccagacccg cggctatggc accggcggca cggtgcacat
     5221 cgtcatcaac aaccagatcg gcttcaccac ctccgacccg cgcgacaccc ggtccacaat
     5281 ctactgctcg gacgtcgtga agatgatcga ggcgccggtg ctgcacgtaa acggcgacga
     5341 cccggaagcg gtcgtgttcg ccactcagat cgcgctcgac ttccgcatgg aattcaagaa
     5401 ggacgtcgtg gtggacatcg tctgcttccg caagctcggt cacaacgagc aggacacgcc
     5461 ggcgctgacc cagccgctga tgtacaagaa aatcgcccag catccaggca cgcgcaagct
     5521 ctacgccgac aagctgatca cccagagcac gatcccggcc gatgccggcg acggaatggt
     5581 caaggcctat cgcgaggcga tggacgccgg ccgccacacc gtcgatccgg tcatctccaa
     5641 cttcaagagc aagtacgcgg tcgactggat gccgttcctg aaccgcaagt ggaccgatgc
     5701 cgctgatacc gccgtgccgc tggctgaact gaagcgcctc gccacccgca tcaccacggt
     5761 gccggacgac ttcaaggtgc atccgctggt cgaaaaggta ttggccgacc gcgccgcgat
     5821 gggccgcggc gagatgaacc tcgactgggg catgggtgaa cacctggcct acgcctcgct
     5881 ggtggcttcc ggctacgccg tgcgcctgac cggccaggat gccggccgcg gcaccttcac
     5941 gcatcgccac gcggtgctgc atgaccagaa gcgcgagcgc tgggaccagg gcacctatgt
     6001 gccgctgcag aacgtatcgg aaaaccaggc gccgtttacg gtgatcgact cggtactgtc
     6061 cgaagaggcg gtgctcggtt tcgagtacgg ctactcgacc gccgagccga atgcgctgac
     6121 gatctgggaa gcccagttcg gcgacttcgc caacggcgcc caggtcgtga tcgaccagtt
     6181 catcagctcc ggcgaagtga agtggggccg cgcctcgggt ctcgtgatga tgctgccgca
     6241 cggctacgaa gggcagggcc cggagcattc ctccgcgcgc atcgagcgct tcctgcaact
     6301 gtgtgccgac aacaacatgc aggtggtgca gccaaccacg gccgcacaga tcttccacct
     6361 gctgcgccgg cagatgatcc gcctgttccg caagccgctg gtcatcatga cgccgaagtc
     6421 gttgctgcgt aacaaggacg ccggttcgcc gctgtccgag ctggccaagg gcactttcca
     6481 gaccgtgatc ggcgaggtcg atgacaagat cgatccgaag aaggtcaagc gcgtgctcgc
     6541 gtgttcgggc aaggtctatt acgacctcat caacgcgcgc aaggaacgcg gcgcctccga
     6601 tgtcgccatc atccggatcg agcagctgta tccgttcccg cacaaggcct tcgcggccga
     6661 gctcaagaag ttcccgaact tcaccgagct ggtgtggacc caggatgagc cgcagaacca
     6721 ggggccgtgg ttccagatcc agcacaacat cttcgagaac ctcgaagatg gccagaagct
     6781 ggcctacgcc ggacgtccgg cctcggcatc gcctgccgtg ggttactacg acaagcacta
     6841 tgcgcagcag aaggccctga tcgaaaccgc attctcgaag ctcaagggct tcgtcctgac
     6901 caagtaaaga cccgacggcg cactagaagc tggtgcgccg tttgaacaaa ccgaaacgga
     6961 gagttaaatg gcaatcattg aagtgaaagt accgcagttg tcggaatcgg tcgcggaagc
     7021 gaccctgctg tcgtggcaca agaaggtcgg cgaagccgtc ggccgtgatg agaacctgat
     7081 cgatatcgaa accgacaagg tggtgctgga actgccggcc ccggatggcg gcgtgctcgt
     7141 cgaaatcctg aaggaagacg gtgcgactgt cgtggcc
//
LOCUS       DATLGF010000032         2750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_143421, whole genome shotgun sequence.
ACCESSION   DATLGF010000032 DATLGF010000000
VERSION     DATLGF010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143421"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VM406_00975"
     CDS             <1..419
                     /locus_tag="VM406_00975"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HVL74559.1"
                     /translation="MRVIALRRLPHRPVLTADTTVEVDGRILGKPANEKEAMEMLRLM
                     SGRTHRVLTAVAVRRDDDHFQLLQVSEVRFAPLTEAMMRAYCMTTEPYDKAGGYGIQG
                     KAAIFVERITGSHSGIVGLPLHETAQLLHQAGIAIL"
     gene            416..1879
                     /gene="rng"
                     /locus_tag="VM406_00980"
     CDS             416..1879
                     /gene="rng"
                     /locus_tag="VM406_00980"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HVL74560.1"
                     /translation="MSEDILINITPQETRVALVLQGAVQELHVERTLSRGMTGNIYLG
                     KVVRVLPGMQSAFIDIGLERAAFLHVADIREARTNDNPNAPLLPIEKLLFDGQSLMVQ
                     VIKDPIGTKGARLSTQISIAGRMLVYLPHDPHIGISQRIEKDVDREQLKSRVKKLLPP
                     DEKGGVIIRTMAEDASEADLQMDIDYLRKTWATIQQEARSTPAPNLLYQDLSLAQRVL
                     RDFVNDDTSTIQVDSRENYQKLQQFGATYTPSVLAKLVHYTGERPLFDLYGVEEEIQR
                     ALGRRVDLKSGGYLIIDQTEAMTTIDVNTGSYVSGRNFDDTIFKTNLEAAHAIARQLR
                     LRNLGGIIILDFIDMENPEHRNAVLVELNKALAKDRTKVSVSPFSALGLVEMTRKRTR
                     ESLAHVLCEVCPACGGKGQVKTARTVCYEILRELLREAKQFNPREFRILASQVVIDMF
                     LEEESQHLAMLGDFIGKPISLQVESLYHQEQYDIILM"
     gene            2243..>2750
                     /locus_tag="VM406_00985"
     CDS             2243..>2750
                     /locus_tag="VM406_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HVL74561.1"
                     /translation="MKLDGRLWIAENGDPLAGHGRIELLARIGSLGSISGAAKNLGIS
                     YKAAWDAVDAMNNLAGEALVERSTGGRGGGGTRLTARGERLVAAFRQIEDEHRRFLDA
                     LGAHIDDFAGTYQVLRRLNMKTSARNQLHGRVAAIRPGAVNDEIEIALRGGDRLFATI
                     TRESTRHLA"
BASE COUNT          544 a          936 c          834 g          436 t
ORIGIN      
        1 cgatgcgggt gatcgcgctg cgccgcctgc cgcaccgccc ggtcctcacc gccgacacca
       61 ccgtcgaggt cgacggccgc atcctcggca agccggccaa tgaaaaggag gcgatggaga
      121 tgctgcgcct gatgtcggga cgcacgcacc gggtgctcac cgcggtggcc gtgcgccgcg
      181 acgacgacca tttccagctg ctgcaggttt ccgaggtgcg ctttgcgccg ctgaccgaag
      241 cgatgatgcg cgcctactgc atgaccaccg agccctacga caaggccggc ggctatggca
      301 ttcagggcaa ggcggcgatc ttcgtcgaac gcatcacggg cagccactcc ggcatcgtcg
      361 gcctgccgct gcacgaaacc gcacaactat tacatcaggc gggcatagca atcctatgag
      421 cgaagacatc ctcatcaata tcacgccgca ggagacgcgc gtggcattgg tgctgcaagg
      481 cgcggtacag gagctgcatg tggagcgcac gctctcgcgc ggcatgaccg gcaacatcta
      541 tctcggcaag gtcgtgcgcg tgctgcccgg catgcaatcg gcgttcatcg acatcgggct
      601 cgagcgcgcg gccttcctgc acgtggccga catccgcgag gcgcgcacca acgacaatcc
      661 gaacgcgccg ctgctgccga tcgagaaact gctgttcgac gggcagtcgc tgatggtgca
      721 ggtgatcaag gaccccatcg gcaccaaggg cgcgcggctg tccacccaga tctcgatcgc
      781 cgggcgcatg ctggtctacc tccctcacga tccgcacatc ggcatctcgc agcgcatcga
      841 gaaggacgtc gaccgcgaac agttgaagag ccgggtgaag aagctgctgc cgcccgacga
      901 gaaaggcggc gtgatcatcc gcacgatggc cgaggacgcc tccgaggccg atctgcagat
      961 ggacatcgac tacctgcgca agacctgggc gacgatccag caggaggcac gcagcacgcc
     1021 tgcgcccaac ctgctctacc aggatctctc cctcgcgcag cgcgtgctgc gcgacttcgt
     1081 caacgacgac acctcgacca ttcaggttga ctcgcgcgag aactaccaga agctgcagca
     1141 attcggcgcc acctacaccc cgtcggtgct tgccaagctg gtgcattaca ccggcgagcg
     1201 tccgctgttc gacctgtatg gcgtggagga ggaaatccag cgcgcgctcg ggcggcgcgt
     1261 ggatctgaaa tccggtggct acctgatcat cgaccagacc gaggcgatga ccacgatcga
     1321 cgtcaacacc ggcagctatg tcagcggccg caacttcgac gacacgatct tcaagaccaa
     1381 tctcgaggcc gcccatgcga tcgcgcgcca gctgcgcctg cgcaacctcg gcggcatcat
     1441 catcctcgac ttcatcgaca tggagaaccc cgagcaccgc aacgcggtgc tggtcgagct
     1501 gaacaaggcg ctcgcgaagg acaggaccaa ggtgtcggtc tcgccgttct ccgccctggg
     1561 actggtcgag atgacgcgca agcgcacccg tgaatcgctc gcgcatgtgc tgtgcgaagt
     1621 gtgccctgcc tgcggcggca agggccaggt caagaccgcg cgcacggtct gctacgaaat
     1681 cctgcgcgaa ctgctgcgcg aggccaagca gttcaatcca cgcgagttcc gcatcctcgc
     1741 ctcgcaggtg gtgatcgaca tgttcctcga agaggaatcg cagcatctcg ccatgctcgg
     1801 cgacttcatc ggcaagccga tctcgctgca ggtcgaaagc ctgtatcacc aggaacagta
     1861 cgacatcatc ctgatgtagg cggcgcgtcg ctgtttcgga ggtattgttg gcggcgatgg
     1921 caacacgtcg taccgcctta gatgtctcgc agctcgattc caattgagcg aacaatcaag
     1981 ctatgtcgct cccgcgcagg cgggagccca gttcgatcgc agcagcacaa ccggcgctcg
     2041 aaccgggaaa caacactgca tgttacttca gttgatcgac tgaaattcca accgaactcg
     2101 tcatcccttc agcgggaaac ggccccctcc tgctgccggc ggtctcaaca cgcccgaccg
     2161 tccgcagaat agctcttccg acccatacct ccccgccgcc gattgcgtta tattccacct
     2221 tatataacgc tttccaccac cgatgaaact cgacggacgc ctctggatcg ccgaaaacgg
     2281 cgacccgctg gccggacacg gccgcatcga actgctcgcg cgcatcggct cgctcggctc
     2341 gatcagcggc gcggcgaaga acctcgggat cagctacaag gcggcctggg acgccgttga
     2401 cgcgatgaac aacctggccg gtgaagcgct ggtcgagcgc agcaccggcg ggcgcggcgg
     2461 cggcggcacc cggctgaccg cgcgcggcga acggctggtg gccgccttcc gtcagatcga
     2521 ggacgagcac cggcgcttcc tcgatgcgct tggcgcgcac atcgacgact tcgccggcac
     2581 ctatcaggtg ttgcgcagac tgaacatgaa gaccagtgcc cgcaaccagt tgcacggccg
     2641 cgtcgccgcg atccggccgg gcgcagtgaa cgacgaaatc gagatcgccc tgcgcggcgg
     2701 cgaccgcctg ttcgccacga tcacgcgcga aagcacacgc catctcgcgc
//
LOCUS       DATLGF010000033         3351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_33458,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000033 DATLGF010000000
VERSION     DATLGF010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33458"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VM406_00990"
     CDS             <1..714
                     /locus_tag="VM406_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HVL74562.1"
                     /translation="CGETGMGRMLEPEFLLEETIAAFQAKRLAGKRVLLTAGPTFEPI
                     DPVRGITNLSSGKMGYALARAAREAGAEVTLVSGPTSLATPFGVHRINVQTAQQMHDV
                     VMSKVDGNDVFIAVAAVADWRVANTSEQKLKKKDATDLPQLIFEQNPDILAAVAASPK
                     RPYCVGFAAESENLLQYGEEKRRKKGIPLLVGNIGHQTFGKDENELVLFDESGSTPLP
                     RGRKQDLARRLIAEIAARI"
     gene            795..1244
                     /gene="dut"
                     /locus_tag="VM406_00995"
     CDS             795..1244
                     /gene="dut"
                     /locus_tag="VM406_00995"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HVL74563.1"
                     /translation="MKTIDLKILDARMKELLPAYATTGSAGLDLRACIDEAIVLKPGD
                     TRLIPTGLAIHIADPGYAAMILPRSGLGHKHGIVLGNLVGLIDSDYQGQLMVSTWNRG
                     TTEFTLNPMERLAQLVIVPVLQVGFNVVEEFTGSERGAGGFGSTGKH"
     gene            1282..2376
                     /locus_tag="VM406_01000"
     CDS             1282..2376
                     /locus_tag="VM406_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HVL74564.1"
                     /translation="MKRAARMLPLAAAVLLGACALPPQIEPGAATAPAPAPAAIPPQA
                     QQPAQPPASPADIQRLQDLVSQQDRLYRVAAPLLINNSELCRSHARALLGFRAKNRFS
                     YSAELSSAAHHLLGLDERLRVTGVIPNSGAARAGIQVGDVLLAVEGKAFPEGEQAESQ
                     AATLLAPLVAEKKVLKLTVLRAQDQLALDVPLTPACAFNIEVGNTDLVNAYGDGFRVL
                     LTRGMLNTLRNDDEVAYVLAREMAHNVLGHAARMNQQAAVGGIIDNLARLQPDMSTMT
                     GMAGLRPVSPDFDSAADRLAIYLLARAGYNIERAAPFWRRFTAQHPVSAQNAYSALHP
                     AIERRIGGIEQAVRDIRNRRANGRPLIPAP"
     gene            2587..>3351
                     /locus_tag="VM406_01005"
     CDS             2587..>3351
                     /locus_tag="VM406_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVL74565.1"
                     /translation="MKLNRWLMMLAAATAAFGATGVAMAQAQDFPNKPIRLIVPFPPG
                     GTTDILARAVGAELNKALGQPVVVENRAGAGGNIGSEAVAKSAPDGYTLLMGTVGTHG
                     INQSLYSKLPFDPIKDFAPVTLVASVPNVLVVNTALAEKNNITDVPSFISYLQKNPGK
                     VNMASSGNGTSIHLAGELFKTMTGTFMVHIPYRGSAPAVTDLIGGQADVMFDNLPSAL
                     PHIKSGRLRAIGVTSANASAALPDVPPIANTGGKLTG"
BASE COUNT          657 a         1152 c         1032 g          510 t
ORIGIN      
        1 tgcggcgaaa ccgggatggg acgcatgctc gaacccgaat tcctgctgga agaaaccatc
       61 gcggcgttcc aggccaagcg cctcgcgggc aagcgggtgc tgctgaccgc gggcccgacc
      121 ttcgagccca tcgatccggt acgcggcatc accaatcttt cgtccggcaa gatgggctat
      181 gcgctcgcgc gtgccgcgcg cgaagccggg gctgaggtca cgctggtttc cggcccgacc
      241 tcgctggcga cgccattcgg cgtgcaccgc atcaacgtgc aaaccgcgca gcagatgcat
      301 gacgtcgtaa tgtcgaaagt cgacggcaac gatgtgttca tcgcggtggc ggccgtggcc
      361 gactggcgcg tcgccaatac cagcgaacag aaactgaaga aaaaagatgc gaccgacctg
      421 ccgcagctga tcttcgaaca gaaccccgac atcctggcag cggtcgcggc aagtcctaag
      481 cgtccttact gcgtcggctt tgccgccgaa tcggagaacc tgttgcaata cggcgaggaa
      541 aagcgccgca aaaagggcat tcctctcctt gtcggcaata tcggccacca gaccttcggc
      601 aaggacgaaa acgagctcgt gctgttcgac gaaagcggca gcacgccact cccgcgcggg
      661 cgcaagcagg acctcgcgcg ccgcctgata gccgaaatcg ccgcgcgcat ctgagcgcct
      721 gcgccgccgc gtccgacgca cagcggcgcg gggaaggcgg cggccgcccc acgacatcca
      781 cccagaagca agccatgaag accatcgatc tgaaaatcct cgacgcccgc atgaaggaat
      841 tgctgcccgc gtatgcgaca accggcagcg ccggcctgga cctgcgggca tgcatcgacg
      901 aagcgatcgt gctcaaaccg ggcgacaccc ggctgattcc gaccgggctg gcgatccaca
      961 tcgccgaccc cggctacgcc gcgatgatcc tgccgcgcag cggcctgggg cacaagcacg
     1021 gtattgtcct ggggaacctt gttggcctga tcgactccga ttaccagggg caattgatgg
     1081 tctccacctg gaaccgcggc accaccgagt tcacgctgaa tccgatggag cgactcgcgc
     1141 agctggtgat cgtgccggtg ctgcaggtcg gcttcaacgt cgtggaggaa ttcacgggca
     1201 gcgagcgcgg tgccggcggc ttcggcagca cgggaaaaca ttgaaggaag acgaatgacg
     1261 gggattgggt acggacggac aatgaaacgg gcggcgcgca tgctgccact ggcggcggcg
     1321 gtcctgctcg gcgcctgcgc cctcccccct cagatcgagc cgggcgccgc taccgcgccc
     1381 gcgcccgcgc ccgcggccat cccgcctcaa gcccagcaac ccgcacaacc gcccgccagt
     1441 cccgccgaca tccagcgtct gcaagacctg gtcagccagc aggacaggct ctaccgagtg
     1501 gcggccccgc tgctcatcaa caattccgaa ctgtgccgca gccatgcgcg cgcactgctc
     1561 ggattccgcg cgaagaaccg cttctcctat tcggctgagc tgtcgagtgc cgcgcaccat
     1621 ctgctcggtc tggatgaacg cctgcgcgtg accggcgtga ttccgaacag cggcgccgcg
     1681 cgcgccggca tacaggtcgg cgatgtgctc ctggccgtcg agggcaaagc cttccccgaa
     1741 ggagagcagg cggaaagcca ggcggccacg ttgctggcgc cgctggtggc ggagaagaag
     1801 gtgctgaagc tgacagtgct gcgcgcacag gaccagcttg cactcgacgt tccgctcacg
     1861 cctgcctgcg cattcaacat cgaagtcggc aacaccgacc tcgtcaacgc ctatggcgac
     1921 ggcttccggg tactgctcac gcgcggcatg ctcaacaccc tgcgcaatga cgacgaggta
     1981 gcctatgtgc tcgcgcgcga gatggcgcac aatgtgctcg gccatgccgc ccgcatgaac
     2041 cagcaggcgg ctgtcggggg catcatcgac aatctcgcgc gcctgcagcc ggacatgagc
     2101 acgatgacgg gaatggcagg actgcgtccg gtttcacccg acttcgactc ggccgccgat
     2161 cggctggcga tctacctgct cgcccgggcc ggctacaaca tcgagcgtgc agccccgttc
     2221 tggcggcgct tcaccgcgca gcacccggta tcggcgcaga acgcgtacag cgcgctgcat
     2281 cctgcaatcg aacgccgtat cggcggaatc gagcaggctg tacgcgacat ccgcaaccgc
     2341 cgggcgaacg gccgcccgct catccccgca ccctgagccg catccgccgc gcgtgcaatg
     2401 cgcgcggcat ggacataccg gtttgctaaa gaccaagcag actgggctac tatcgaggta
     2461 ttcttgagcc tcgcgccgtt ccatcggtct gataccggcg caaacgaaaa gatgccgggg
     2521 aacaccggcc cgctcaagtt cccagccgca tccttgcgtg cggcaactta accccaagga
     2581 ggagacatga agctcaaccg ctggttgatg atgctcgccg cggcgaccgc ggcatttggc
     2641 gccaccggcg tcgcaatggc acaagcgcag gattttccga acaagccgat ccggctcatc
     2701 gttccattcc cgccgggcgg caccacggac atcctggcac gcgcagttgg tgccgaactg
     2761 aacaaggcgc tcggacaacc cgtcgtagtc gagaaccgtg ccggcgccgg cggcaacatc
     2821 ggctccgagg ctgtcgcgaa atccgcgccc gacggctaca cgctgctgat gggaaccgtc
     2881 ggcacgcacg gcatcaacca gtccctttat tccaagcttc cgttcgatcc gatcaaggac
     2941 ttcgcgcccg tgactctggt tgcgtcggtg cccaacgtac tggtcgtaaa caccgcgctg
     3001 gcagaaaaga acaacatcac cgacgtcccg agcttcatca gctatctgca aaagaatccg
     3061 ggcaaggtca acatggcttc gagcggcaac gggacgtcaa tccatctcgc cggcgagttg
     3121 ttcaagacca tgaccggaac cttcatggtc cacattccgt atcgcggctc cgcgccggcg
     3181 gtgaccgatc tgatcggcgg ccaggccgac gtgatgttcg acaacctgcc gtccgcgctc
     3241 ccgcacatca agtcgggccg gctgcgtgcc atcggcgtca ccagcgccaa tgccagcgca
     3301 gcgctgccgg atgtaccgcc gatcgcgaac accggcggca agctgaccgg c
//
LOCUS       DATLGF010000034         4375 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_96722,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000034 DATLGF010000000
VERSION     DATLGF010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96722"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1715)
                     /locus_tag="VM406_01010"
     CDS             complement(87..1715)
                     /locus_tag="VM406_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HVL74566.1"
                     /translation="MKVRILQACAVATCVIVVSAALAGIAQWSGWQPGVFTLVFAGCI
                     AGIAVSLLLLRKNASEAAPLIEKIGAEIDHLMIGAAETSHFVDSIRKRVDHDLETTRE
                     IAARATHTARATEQMAHNAERASLVAADVRDESVAGRGEVDRGLDQISNARQDAQAAS
                     EMMGKLQEKSRRIHVITEVINEIAARTNLLALNAAIEAARAGEHGRGFAVVAGEVRQL
                     AQRTKAATDDIGAMVREITEEAERATSGMAALTAKVMEAAQNVEKVHGFLSNIECSAG
                     ASQEEIAQIAAASREHVATTQLIADAIGRIQDGMLATEADLPVVSKSAMQLSERAESL
                     FVAATDCNAGTSHDEIRALAQQVAAQVGRLFEQAIASGKIGEAALFDRTYTPIPNTNP
                     PKHRTAFDAFTDQVLPAIQEPILERMPHLAYAGAVDNNGYFPTHNRKYCQPLTGNYET
                     DLVNNRTKRIFNDRTGARCGANTKPFLLQTYKRDTGEVMHDLSAPIYVNGRHWGGFRI
                     GYRSGSAPRPAPPQSAPRAATPPSLPAKQGKARS"
     gene            complement(1843..3234)
                     /gene="fumC"
                     /locus_tag="VM406_01015"
     CDS             complement(1843..3234)
                     /gene="fumC"
                     /locus_tag="VM406_01015"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HVL74567.1"
                     /translation="MADTRAERDSLGMVEVPADRLWGAQTQRSLEHFRISTERMPAEL
                     VSALAMVKRACAVVNRNLGRLETKTANAITAAAQEVLTGKWPDEFPLAVWQTGSGTQS
                     NMNMNEVLANRASELLGGARGSDRLVHPNDHVNMGQSSNDVFPTGMHVAAARAVANDL
                     LPALDALRATLQARAEAFADIVKIGRTHLQDATPLTLGQEFSGYVAQLDQARRGIEAS
                     LSGLYELAVGGTAVGTGLNTHPEFGERVSAQLAEDTGLPFVSAPNKFAALAGHDALVF
                     AHGALKTLAAALTKIANDVRWLASGPRSGLGEIRIPENEPGSSIMPGKVNPTQAEALT
                     MLCAQVFGNDVAVTFGGASGNFELNVYKPLIAHNFLQSVRLLADGMRSFDAHCARGIE
                     ADRVRIAELMERSLMLVTALAPHIGYDRAAQIAKLAHRDGCTLKQAALALGYVSEADF
                     DRWVRPEAMVSPG"
     gene            complement(3335..>4375)
                     /locus_tag="VM406_01020"
     CDS             complement(3335..>4375)
                     /locus_tag="VM406_01020"
                     /inference="COORDINATES: protein motif:HMM:NF017388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HVL74568.1"
                     /translation="ALDVTDPDSPTLLWEIDQTTESNLGFSFAKPVITKLNDGTWVVL
                     FTSGYNNISPGDGKGYLYVRNAVTGAHIRRIATNVGSTDNPSGLGKIASWADKPEKNN
                     LTTFTYGGDLQGNLWRFDINAGTATRMAILRDAAGAVQPITARPELGAVSGRRIIFVG
                     TGKYLELSDLEDAQTQTLYAIRDDLSAGTLDNPRTALVEQTLTTTGATRKVSSNAIDL
                     RRDRGWYIDFPDSGERVNVDIRLDSGTLIVPTTVPSATVCEPGGYGWLNYFNYLTGSV
                     VPGAEDGLSGQRYNAPIVGVNTYRLPNGKRIVTAVTANNPTPEKPPKGTGGDLPDAGF
                     RGKRVIYREISP"
BASE COUNT          722 a         1405 c         1428 g          820 t
ORIGIN      
        1 cctcagcgca tatgcgcgac ttgcccgccg gtgccgcgcg gcggcgacac aaggatctca
       61 acccccgccg aagtggctga cccgggctag gatctggcct tgccctgctt ggcaggcaag
      121 ctcgggggcg tggctgcccg cggcgcggac tgcggaggcg ccgggcgagg cgccgaaccg
      181 gaacgatagc cgatccggaa gccgccccaa tgtctgccgt ttacatagat cggcgccgag
      241 agatcgtgca tcacctctcc ggtgtcgcgc ttgtaggtct ggagcaggaa gggtttggta
      301 ttggcgccgc agcgtgcgcc ggtgcggtcg ttgaagatgc gcttggtgcg attgttcacg
      361 agatcggtct cgtagtttcc ggtcagcggc tggcaatact tgcggttgtg tgtcgggaag
      421 tagccgttgt tgtcgaccgc tcccgcatac gcaagatgcg gcatccgctc gaggatcggc
      481 tcctggatcg ccggcagcac ctggtcggtg aatgcgtcga aggcagtacg gtgctttggc
      541 gggttcgtgt tcggaatagg cgtgtaggtg cggtcgaaca atgccgcctc gccgatcttc
      601 ccggaagcga tcgcctgctc gaacagccgt ccgacctggg cggccacctg ctgcgccagc
      661 gcgcgaatct cgtcgtgcga ggtgccggca ttgcagtcgg tggcggcaac gaacaggctt
      721 tcggcacgct cgctgagctg catggccgac ttcgatacga cgggcaggtc ggcttccgtc
      781 gccagcatgc cgtcttgaat gcggccgatc gcgtccgcga tcaactgcgt cgtcgccaca
      841 tgttcacggg aggccgcagc gatctgggcg atctcttcct gtgaagcccc ggccgaacac
      901 tcgatattgg acaggaaacc gtggaccttt tccacgttct gggccgcttc catcaccttg
      961 gcagtgagcg ccgccatgcc cgaggtcgcg cgttcggctt cctcggtgat ctccctgacc
     1021 atcgcgccga tgtcatcggt ggccgccttg gtccgctgcg ccagctgcct cacctcgccg
     1081 gccaccaccg caaatccccg cccgtgctcg ccggcgcgcg cggcctcgat cgccgcattg
     1141 agcgccagca gattggtgcg cgcggcgatc tcgttgatca cctcggtgat cacatgaatg
     1201 cggcgcgact tctcctgcag cttgcccatc atctcggagg ccgcctgcgc gtcctggcgc
     1261 gcattgctga tctggtccag gccgcggtcg acttcgccgc gaccggccac gctttcatcg
     1321 cgcacatcgg ctgcgacgag ggaggcgcgc tcggcgttgt gcgccatttg ttcggtggca
     1381 cgcgcggtat gggtggcacg cgcggcgatt tcgcgggtgg tctccagatc gtggtcgacg
     1441 cgcttgcgga tcgaatcgac gaagtgcgag gtttcagcgg cgccgatcat caggtggtcg
     1501 atctcggcgc cgatcttctc gatcagcggc gccgcttcgg acgcgttctt ccgcaggagc
     1561 agcaaggaca ccgcgatgcc ggcgatgcag ccggcgaaca ccagggtgaa gaccccgggc
     1621 tgccaacccg accattgcgc aatccccgca agggcggcgc tcacaacgat gacgcatgtg
     1681 gcaacggcgc aggcctggag gatgcgtacc ttcatggtgt ctccgcatgt tgtcgtttgc
     1741 tctgatgata gttgaggttt ggaaacactc caagccatct cgaagcagga cgcagcgaat
     1801 gcgacaacag catggccgcc ggcgtgcgcc gaaggcgacc gttcagcccg ggctgaccat
     1861 cgcctccggc ctcacccagc gatcgaaatc ggcttcggag acatagccga gggccagcgc
     1921 cgcctgcttg agcgtgcagc cgtcgcgatg cgcgagcttg gcgatctgcg cggcgcggtc
     1981 gtagccgatg tgcggcgcca atgcggtcac cagcatcagc gagcgctcca tcagttcggc
     2041 gatccggacg cggtccgcct cgatgccgcg ggcgcaatgg gcgtcgaagc tgcgcatgcc
     2101 gtccgcgagc agacgcacgc tctgcaggaa gttgtgggcg atgagcggct tgtagacgtt
     2161 gagctcgaaa ttgccgctcg cgccgccgaa ggtcacggcc acatcgttgc cgaacacctg
     2221 cgcgcagagc atggtcagcg cttccgcctg cgtcggattc accttgccgg gcatgatcga
     2281 gctgccgggc tcgttttcag ggatcctgat ctcgcccagc ccggaacggg gaccggacgc
     2341 gagccagcgc acatcgttgg caatcttcgt cagagccgca gccagcgtct tcaacgcccc
     2401 atgcgcgaac acgagtgcgt catgtccggc cagcgcggcg aacttgttcg gcgccgaaac
     2461 gaatggaagg ccggtatctt cggcaagctg ggcggaaacc cgctctccga attcaggatg
     2521 ggtgttcagg ccggtcccca ccgccgtgcc gccgacagcc agttcataca ggccggaaag
     2581 actggcctcg atgccgcggc gcgcctggtc gagctgggcc acataacccg agaactcctg
     2641 ccccagcgta aggggggtgg catcctgcag atgggtgcgg ccaatcttga cgatgtcggc
     2701 gaaggcctcg gcgcgggcct gcaaggtcgc gcgcaacgca tccagcgccg gcagcagatc
     2761 gttcgcaacc gcacgcgcgg ccgccacgtg catcccggtc gggaacacgt cgttggacga
     2821 ctgccccatg ttcacatgat cgttcggatg cacgagccga tcagatccgc gcgccccgcc
     2881 gaggagttcc gaggcacgat tggccagcac ctcgttcatg ttcatgttgc tctgtgtgcc
     2941 ggaaccggtc tgccataccg caaggggaaa ttcatccggc cacttgccgg tgaggacttc
     3001 ctgggctgcc gcggtgatcg cattggctgt cttcgtctcg agtcgtccga gattgcgatt
     3061 gaccaccgcg caagcccgct tgaccatggc cagcgcgctc accaattctg ccggcatgcg
     3121 ttccgttgag attcggaaat gctcgagcga gcgctgggtc tgggcgcccc acaatcggtc
     3181 cgcgggcacc tccaccatgc ctagggagtc ccgttctgct ctggtatctg ccatggcgca
     3241 tcaacaggaa aggttcatgc tcccttgggt ctgtgccaaa cgagaaggtt cgcggcttgt
     3301 cgtaccgggc tgcggcacga caagcgggcg caccctacgg gctgatttcg cgatagatca
     3361 cgcgcttgcc gcgaaaaccc gcatcgggaa gatcgccgcc cgtgcccttc gggggtttct
     3421 ccggcgtcgg attgttcgcc gtcaccgcgg tcacgatgcg cttgccattc ggcaatcgat
     3481 aggtgttgac gccgacgatg ggcgcgttgt agcgctgccc ggacagcccg tcttccgcac
     3541 ccggcaccac gctgccggtc agatagttga agtagttcag ccagccgtag ccccccggct
     3601 cgcagaccgt cgccgaaggc acggtggtcg gcacgatcag cgtgccggaa tcgagccgga
     3661 tgtccacgtt tacccgctcg ccggaatcgg gaaaatcgat ataccagccc cgatcgcgcc
     3721 tgagatcgat ggcgttggac gacaccttcc ttgttgcgcc tgtcgtcgtc agagtctgct
     3781 cgacgagcgc cgtgcgcgga ttgtccagtg tgccggcaga aagatcgtcg cgtatcgcgt
     3841 acaaggtctg cgtctgggca tcctcgagat cggaaagctc gagatacttg ccggtgccga
     3901 caaaaatgat gcgcctgccg gagacagcac caagttcggg gcgtgcggtg atcggctgca
     3961 cggccccggc ggcgtcgcgc aggatcgcca tgcgcgtcgc cgtgccggca ttgatgtcga
     4021 agcgccacag attgccctgc aggtcgccgc cataggtgaa ggtggtcagg ttgttcttct
     4081 cgggcttgtc ggcccagctc gcgatcttgc cgagaccgga tggattgtcg gtggatccga
     4141 cgttcgtggc gatgcgtcgg atatgcgcgc cggtcaccgc attgcgcacg tacagatagc
     4201 ctttaccatc gcccgggctg atgttgttgt aacccgaggt gaacaagacg acccaggtcc
     4261 cgtcattgag cttcgtgatc acgggcttgg cgaagctaaa accaagattg gattccgtcg
     4321 tctggtcgat ttcccacaac agcgtgggtg agtccgggtc cgtcacatcc aacgc
//
LOCUS       DATLGF010000035         3054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_17725,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000035 DATLGF010000000
VERSION     DATLGF010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17725"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VM406_01025"
     CDS             complement(<1..206)
                     /locus_tag="VM406_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74569.1"
                     /translation="MLLRNSLWNLSGSAIPMVVALATIPILIGALGVEGFGIVTLVGS
                     IIGYFGVLDINLQAGSIKYLAEHHA"
     gene            complement(193..1608)
                     /locus_tag="VM406_01030"
     CDS             complement(193..1608)
                     /locus_tag="VM406_01030"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HVL74570.1"
                     /translation="MNSSLSTGAVITNLLAALFVIPIVWGFFIKAPKLVMLGFVALLF
                     MFSDSTWGQLQIENTIYSRGSGMFYFSLVNLGLLAAGMAALIKRLANPGGPQLAPPMA
                     KYFLTFGFILFGHMVVGLMQGIELPVILGYNGVLNVLNMFVFMYLVIMAFDSEENGQQ
                     LMLAVVLLAAIRSIYGGVRYVLFGGDSANPYRNFEGMDVRIYFFDIGDNFVAALGAFC
                     AAWLLTSPEVRLSLLKRALLFGFLLLQIAAVALSFRRSSLIGLALMFLFLFLRIPGQR
                     KLFFAMLAAGVLFIVATIFYQQRLQFASDEGLIASLIYDITSERDIRYGRFYELWLAA
                     QSMEDHWLFGRGTWGTFRGDTQALAFHTDFGFVHSGFGHLVLKTGLVGLAVFCALLAS
                     FTMFYLRHRNALVGNARLMADAGFAGFLFWIPTLLIGTPIIEIRTMLLFGLTLALPFI
                     AVGVQQRQEQAYPYHPAYAAA"
     gene            complement(1716..2669)
                     /locus_tag="VM406_01035"
     CDS             complement(1716..2669)
                     /locus_tag="VM406_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HVL74571.1"
                     /translation="MKQTIFVAGHRGLVGSAIVRALEARGDADIVTRTHAQLDLTDQA
                     QVRAFFAEQKIDQVYLAAARVGGIHANNTYPADFIRDNLLIQTNVIDAAWQSGVKRLL
                     FLGSSCIYPRMAPQPMPEACLMTGELEPTNEPYAIAKIAGIKMCESYRRQYGADFRSV
                     MPTNLYGPGDNYHPENSHVIPALIRRFHEAKQSGATSVAIWGTGTPRREFLFVDDMAS
                     ACLHVMNLDRATYDAHTTPQLSHINVGTGQDVTIREVAELVRKVTGYEGDIAFDTSRP
                     DGTPRKLLDTMKLGALGWRPQVQLEEGLRMTYASFQAEYGS"
     gene            complement(2691..>3054)
                     /locus_tag="VM406_01040"
     CDS             complement(2691..>3054)
                     /locus_tag="VM406_01040"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HVL74572.1"
                     /translation="LYAWAALACYLSMIVLGSIPGQAESLSEVVHDKMLHFVAYGVLS
                     VLVYGAVRGRLWVRGIGTIAVVGLLGAIDEGIQSFLPYRQASWADWQVDLIAALLTVT
                     VMTTLQVAQPRRGRGPLL"
BASE COUNT          627 a          910 c         1020 g          497 t
ORIGIN      
        1 gcatgatgtt cggcgaggta cttgatcgag ccggcctgca ggttgatgtc gagcacgccg
       61 aagtagccaa tgatcgagcc gaccagcgtg acgatgccga agccctccac accaagcgcg
      121 ccgatcagga tgggaatggt ggccagcgcc accaccatcg gaatcgcgga gccggacaga
      181 ttccagagcg agttacgcag cagcataggc ggggtgatag gggtaggcct gttcctgccg
      241 ttgttgtaca ccgacggcga tgaatggcaa ggcgagcgtc aggccgaaca gcagcatcgt
      301 gcggatctcg atgatcggtg taccgatcaa cagcgtcgga atccagaaca ggaaaccggc
      361 gaagccggca tcggccatca ggcgcgcatt gccgaccagc gcattgcgat gacgcaaata
      421 gaacatcgtg aagctcgcca gcagcgcgca gaagacggca aggccgacga ggccggtctt
      481 gaggaccagg tggccgaagc cgctgtgcac gaaaccgaaa tcggtgtgga acgcaagcgc
      541 ctgtgtatca ccgcggaagg tgccccaggt gccgcgtccg aacagccagt ggtcctccat
      601 gctctgtgcg gccagccaca gttcatagaa gcggccgtag cggatgtcgc gctcggaggt
      661 gatgtcgtag atcagcgagg cgatcaggcc ctcgtcgctg gcgaactgca ggcgctgctg
      721 atagaagatg gtggcgacga tgaacagcac gccggcggcg agcatcgcaa agaaaagctt
      781 gcgctggccc ggtatgcgca ggaacaggaa caggaacatc agggccaggc cgatcagcga
      841 agaacggcgg aacgacagtg cgaccgcggc gatctgcagc agcaggaagc cgaacagcaa
      901 cgcgcgtttc aggagcgaca ggcgcacctc gggcgaggtc agcagccatg ccgcgcagaa
      961 ggcgccgagc gccgcgacga agttgtcgcc gatgtcgaag aaatagatgc gcacgtccat
     1021 cccttcgaaa ttgcggtacg ggttggccga atcgccgccg aaaagcacgt agcgcacgcc
     1081 gccgtagatc gagcggatcg cggccaggag cacgacggcg agcatcagct gctggccgtt
     1141 ctcttcgctg tcgaacgcca tgatcaccag gtacatgaac acgaacatgt tcaggacgtt
     1201 caggacgccg ttgtagccca ggatgacggg caattcgatc ccctgcatca ggccgaccac
     1261 catatggccg aacaggatga agccgaaggt caggaaatac ttcgccatcg gcggcgcaag
     1321 ctgggggccg ccgggattgg cgagccgctt gatgagggcg gccatgccgg ctgcgagaag
     1381 tccgagattg accagtgaga agtagaacat ccccgaaccg cgcgaataga tcgtgttctc
     1441 gatctgaagc tggccccatg tcgagtctga aaacatgaac agcagtgcga cgaagccgag
     1501 catcaccagc ttgggtgcct tgatgaagaa gccccagacg atagggatga cgaacagcgc
     1561 ggccagcagg ttggtgatga ccgcgccggt tgaaagcgat gaattcatgg tgtgctcgat
     1621 gaaaaaccgg ccgggtggcc gccacgcgga gcgggcacgg gaaggaccgg ccgggcaggc
     1681 agcgcgccac aggggcgcgc ggcgcgggca ggaggctacg aaccgtactc ggcctggaag
     1741 ctcgcgtagg tcatgcgcag gccttcttcc agttgtacct gcgggcgcca gcccagcgcc
     1801 ccgagcttca tcgtatccag cagcttgcgc ggcgtgccgt cgggccggct cgtatcgaat
     1861 gcgatgtcgc cttcgtatcc ggtcactttc cgtaccagtt ccgcaacctc tcgtattgtc
     1921 acgtcctggc ccgtgcccac attgatgtgc gataactgag gggtggtgtg cgcatcatag
     1981 gtggcgcgat cgagattcat cacgtgcagg caggcgctgg ccatgtcgtc gacgaacagg
     2041 aattcacggc gcggcgtgcc ggtgccccag atcgcgacgc tcgtcgcgcc gctttgcttt
     2101 gcctcgtgga atcgccggat cagggcgggg atcacatggc tgttctccgg gtgatagttg
     2161 tcgcccgggc cgtacaggtt ggtcggcatc acgctgcgga agtccgcccc gtactggcgg
     2221 cgatagctct cgcacatctt gatgcccgcg atcttcgcga tggcgtacgg ttcgttggtc
     2281 ggctccagtt cgccggtcat caggcatgcc tcgggcatcg gttgcggggc catgcgcgga
     2341 tagatgcacg acgaacccag gaacagcaac cgcttgacgc cgctttgcca ggccgcgtcg
     2401 atgacgttgg tctggatcag caggttgtcg cggatgaagt cggcgggata agtgttgttt
     2461 gcgtggatgc cgcccacgcg cgctgccgca agatagacct ggtcgatctt ctgctccgcg
     2521 aagaaggcgc gcacctgggc ctggtccgtc aggtcgagct gcgcatgcgt gcgggtgacg
     2581 atgtcggcgt cgccgcgcgc ctcgagcgcg cgcacgatgg ccgagcccac cagtccgcgg
     2641 tggccggcta cgaatatggt ctgtttcata tcggtgttca tgaggctttc tcagagtagc
     2701 ggcccgcggc cccggcgggg ctgcgcgacc tgtagggtag tcatcacggt gacggtcagc
     2761 aaagctgcaa tcaggtcgac ctgccagtcc gcccagctcg cctgccggta gggcaggaat
     2821 gactggatgc cttcgtcgat cgcgccgagc aggccgacca cggcaatggt gccgatcccg
     2881 cgcacccata acctgccacg caccgcaccg tagacaagca cgctcaacac cccgtaggcg
     2941 acgaagtgca gcatcttgtc gtgcacgacc tcggacagcg attccgcctg gccgggaatc
     3001 gaaccgagca cgatcatgga cagataacag gcaagggctg cccatgcata gagc
//
LOCUS       DATLGF010000036         4255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_33537,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000036 DATLGF010000000
VERSION     DATLGF010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33537"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..574
                     /locus_tag="VM406_01045"
     CDS             53..574
                     /locus_tag="VM406_01045"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HVL74573.1"
                     /translation="MVRQNGQWSEPLFYNLGGISIGLQAGVEAGDVAFVLNNQRAVDN
                     FLQRNNWSLDAHAGLTVGTWAGKAQADAGRGDITIWTDAAGISGNLAVSIANINFDQE
                     ETYAYYGRQVAVQDVVGGRVTNPHSRILQQTLAAVAGPTATGAAATGAGAAGSGASGY
                     GHQGGQQDMAPSR"
     gene            complement(627..1100)
                     /locus_tag="VM406_01050"
     CDS             complement(627..1100)
                     /locus_tag="VM406_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74574.1"
                     /translation="MLSRRSCFRHFPVTGALLAALNIGPAGAQPTTGPEDTLITPVVP
                     PMVIIAPSEIRSDPTFARGCWVRLFPAQDYKGQDDLTVAGPTDLPSLRSPGGGLMWAQ
                     RAESVLVGPKAVVTLYESPGFRGKYATLGPGAQRARLREDLAFPQSVDALRIRCK"
     gene            1483..2121
                     /locus_tag="VM406_01055"
     CDS             1483..2121
                     /locus_tag="VM406_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HVL74575.1"
                     /translation="MSRFHLMSVWSVALALSGPGIASGKSLDAIDCRLHPGTLCADMN
                     LSGLRAAAMDLSNSQFSGSRLVGARLENTNLSHANLTRADLSKANLEAARLVEADLRA
                     ANLSGANLQRARLDGANLQNVRMLGGDLRQASLRNADFSRADLPASLMQHAQMQRADF
                     NGANLTKVALNGANLAGANFTNANLSGADLRGAVLEGAIFSNTHTTGCRGCP"
     gene            complement(2149..2961)
                     /locus_tag="VM406_01060"
     CDS             complement(2149..2961)
                     /locus_tag="VM406_01060"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HVL74576.1"
                     /translation="MAPAAHAAGVVQRLRLFSLHVPGASFARKESIMMHLKGFPQSTR
                     AWAVGLLLGAASFCATGGEHVGGTTGAGAEAPTGAVQPSTVQVSEGDRQIMREIAQAN
                     LTEIHASRLALERAQDQEVRQFAQRMIEDHTRLHERLADLARVKNVQLPDTPAREQQQ
                     MIERLGKLQGAQFDRAYREQIGEQAHEKTHDMMRRSITRAEDPQLMALITQALPVIEN
                     HLADAEQMHGHASRTGATGGTEPAAATGGANGGNTSTQEPVSGTAGDTGTPR"
     gene            3228..>4255
                     /locus_tag="VM406_01065"
     CDS             3228..>4255
                     /locus_tag="VM406_01065"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVL74577.1"
                     /translation="MVSQKPVILITGAAGRLGSAIIAALGDDYTIVGFERKCPKTPFD
                     CIEADIGSDEGTARACAQLRERYGSRIASVIHLAAFYDFSGEDNPLYESVTVQGTARL
                     LRALQGFEVEQFVYASTMLVHAPSEPGSPINEDSPIEPKWAYPQSKVRAEHEVRTQRG
                     DIPAVILRIAGVYTDYCEVPSLATQMQRIYERQMQSRVFPGNASHGQAFVHIEDVAAA
                     FKACVERRGRLPEETAILIGEPVTESYEAMQNLLGRLIHGEPWHTREIPKSVARTGAW
                     LEDKMEAVIPDAIDRGIEPFIKPFMVDLADDHYELDITRARTLLDWNPQHRLRRCLPM
                     MVGELRQNP"
BASE COUNT          755 a         1356 c         1389 g          755 t
ORIGIN      
        1 gagacagcgg gcggcgcttg ttctcggagg gcgcggcggg gaaggcgtgc tgatggtgcg
       61 ccagaacggc caatggagcg aacccttgtt ctacaacctc ggagggatca gcatcggcct
      121 gcaggccggc gtggaggcgg gcgacgtcgc ctttgtgctg aacaaccagc gcgcggtcga
      181 taatttcctg cagcggaaca actggtcgct cgacgcgcat gccggcctga ccgtcggcac
      241 ctgggcaggc aaggcgcagg cggacgccgg ccgcggcgac ataacgatct ggaccgacgc
      301 cgccggcatc tccggaaatc tcgcggtgag cattgccaac atcaatttcg accaggaaga
      361 aacctacgcc tactatgggc gtcaggtggc ggtgcaggat gtggtgggcg gccgggtgac
      421 gaatccgcat tcgcgcattt tgcagcagac acttgctgcg gtcgccgggc ccacggctac
      481 cggcgcagca gcgaccggtg ccggtgccgc aggctccgga gccagcggat acgggcatca
      541 gggcggccag caggacatgg caccaagccg ttgacacagg caggctccac ggcctgccgt
      601 atggcggcgc cggggagccg taaaccctat ttgcaacgta tgcgcagcgc gtcgactgat
      661 tggggaaacg caagatcctc gcgcagccgc gcgcgctgcg cgccagggcc tagcgtcgcg
      721 tacttgcccc ggaaacctgg cgactcgtaa agcgtgacga ctgctttcgg ccctacaagt
      781 acgctttcgg cccgttgcgc ccacatcagc ccgccacccg ggctgcgaag tgacggaaga
      841 tcggtcggtc cggccactgt caagtcgtcc tgtcccttgt agtcctgtgc cggaaacaga
      901 cgcacccagc agccgcgcgc gaacgtcgga tcggaacgga tttcgctcgg tgcaatgatg
      961 accatcggcg ggacgaccgg cgtgatgagc gtgtcctccg gaccggtcgt gggctgcgcg
     1021 ccggcaggac cgatgttcag ggccgcaagg agtgcgcccg taaccgggaa atgtcgaaaa
     1081 caagagcggc gagagagcat gggtttctcc ttcggcaatg gctggagggc tatgtcgtcg
     1141 cttccgatcg cccatcctct gcgcagcttc gatgagacgg tgcccgccga agttcgtgcg
     1201 ggagctgccc gactgccaat gcattgcctg cctgtcgttg caggtgaacg ccgacgccgg
     1261 ccatgggccg agcgggcacg cgcatgttca atccatgtca aacgggaggg ccgaccggtc
     1321 tggaaccgca tgcggcacca ccgatccaga agagggatcc tgtccgttgt atgcaggggg
     1381 atttgttttg gttcagcgcc aagcgttggc ggtacaccgt gctcccgcgg cccggccggg
     1441 gccagcacct ttgtcgcccc ggcgcgcgca ggagacaacg taatgagccg attccacttg
     1501 atgtcggttt ggagcgtcgc gctggcactt tccggcccag gcattgcgtc cggaaaaagc
     1561 ctggatgcga tcgactgtcg gcttcaccca ggtaccttgt gcgcggacat gaatctgagc
     1621 ggcttgcgcg ctgcggcgat ggatctgtcg aattcccagt tctccggatc acgtctggtc
     1681 ggggcacgat tggagaacac caacctttcc catgccaatc tgacccgcgc cgatctcagc
     1741 aaggccaatc ttgaagcagc gcggctcgta gaggcggatt tacgcgcagc caacctgagt
     1801 ggagcgaatc tccagcgcgc ccggcttgac ggcgcgaacc ttcagaacgt gcgcatgctc
     1861 ggaggagacc tgcgccaggc atcgctgcgc aatgccgact tttctcgcgc cgacctgcct
     1921 gcgtccctga tgcagcatgc gcaaatgcaa cgtgcggatt tcaacggggc aaacctcacc
     1981 aaggtcgcgc tgaacggcgc gaacctggct ggagcaaact ttaccaatgc caaccttagc
     2041 ggggcggatc ttcgtggagc agtgctcgag ggagcaatat tttccaacac gcacacgacg
     2101 ggttgcaggg gatgtccttg aacagccgca ccggactgac cgcctgaatc agcgaggcgt
     2161 tcccgtgtcg cctgccgtgc cggacaccgg ctcctgcgtg gatgtgtttc cgccgttggc
     2221 accgccggtt gcggcagcag gttcggttcc gccagttgca ccggttcgcg atgcatgccc
     2281 gtgcatttgt tccgcatcgg caaggtggtt ctcgatcacc ggaagtgcct gggtaatgag
     2341 tgccatgagc tgcgggtcct ccgcccgcgt aatcgagcgc ctcatcatgt cgtgcgtctt
     2401 ctcgtgcgct tgttcgccaa tctgctcgcg gtaggcgcga tcgaactgcg caccctgcaa
     2461 tttgccgagc cgctcgatca tctgttgctg ttcacgggcc ggcgtgtccg gaagctgaac
     2521 gttcttgacc cgggcgaggt ctgcaagacg ttcatggagg cgcgtgtggt cctcgatcat
     2581 gcgctgcgcg aactggcgga cttcctgatc ctgggcgcgc tcaagcgcca gccggctggc
     2641 gtggatctcg gtcaggttcg cctgggcaat ctcgcgcatg atctggcggt ccccctcgct
     2701 gacctggacc gtgctcggct gcacggcgcc ggtcggcgct tcggcgccgg cgcccgtggt
     2761 gccgccgaca tgctctccgc ccgtcgcgca gaaggacgcg gcgccgagca gcaggccgac
     2821 ggcccaagcg cgcgtagact gtggaaaacc tttcagatgc atcatgatgc tctcctttct
     2881 ggcgaagctt gcgccgggaa catggagtga aaaaagacgc aatcgctgta ccacgcctgc
     2941 ggcatgggca gcgggcgcca ttcggacagc cgcaagccga cgctgccgcg tcggcacacg
     3001 cggcgcttaa ttttttacaa ccgcgctgaa aaaaggtgtg gtggctgcat cgcctgtgca
     3061 ttgactccgg gcattgtcct atccatcact gctcgcgtcc gggacagtgt tggcgaccga
     3121 gcccggcatt ctgcgcgtta cgtgtcccgc ctttgtttcc gtcacgattt gcatcgcaag
     3181 tggcacgcat cctgcctgta tgaaaaatac tgaccaggag gtgcggcatg gtgtcgcaaa
     3241 aacccgtgat tctcatcacc ggcgcggccg gacgtctggg cagcgcgatc atcgccgcgc
     3301 tgggagacga ctacacgatc gtcggcttcg agcggaagtg cccgaagacg ccgttcgatt
     3361 gcatcgaagc cgacatcggc tccgacgagg gtaccgcacg tgcctgcgcg caactgcgcg
     3421 aacgctacgg cagccgcatc gcctcggtca tccacctggc cgccttctac gacttttccg
     3481 gggaggacaa cccgctctac gagtccgtta ccgtgcaggg cacggcgcgg ctgctgcgtg
     3541 cgctgcaggg cttcgaggtc gagcagttcg tgtacgcgag cacgatgctg gtccatgcgc
     3601 cgagcgagcc cggttcgccg atcaacgagg attcgccgat cgagccgaag tgggcgtatc
     3661 cgcagtccaa ggtgcgcgcg gaacacgagg tgcgcacgca acgcggcgac attccggcgg
     3721 tgatcctgcg cattgccggc gtctacaccg attactgcga ggtcccgtcg ctggccaccc
     3781 agatgcagcg catctacgag cgccagatgc agagccgcgt gtttcccgga aatgcctcgc
     3841 acggccaggc cttcgtgcat atcgaagatg tggcggccgc attcaaggcc tgcgtcgagc
     3901 gtcgcggccg gttgccggaa gaaaccgcga tcctgatcgg cgagccggtg accgagagct
     3961 atgaagcgat gcagaacctg ctcggccgcc tgatccacgg cgagccctgg catacgcgcg
     4021 agatcccgaa gtccgtggcc cgcaccggag cctggctgga ggacaagatg gaagcggtga
     4081 tcccggacgc gatcgaccgc ggcatcgagc ccttcatcaa gcccttcatg gtcgatctcg
     4141 ccgacgacca ctacgagctc gacatcacgc gggcgcgcac gctgctggac tggaatcccc
     4201 agcatcgcct gcgccgctgc ctgccgatga tggtgggaga actgcgccag aaccc
//
LOCUS       DATLGF010000037         8849 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_175389, whole genome shotgun sequence.
ACCESSION   DATLGF010000037 DATLGF010000000
VERSION     DATLGF010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 8849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8849
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175389"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="VM406_01070"
     CDS             <1..165
                     /locus_tag="VM406_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HVL74578.1"
                     /translation="IIASGQADMVALARGILYDPRWPWHAAARLGAQVDAPPQYWRSQ
                     PREVKDLFRK"
     gene            complement(166..822)
                     /locus_tag="VM406_01075"
     CDS             complement(166..822)
                     /locus_tag="VM406_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74579.1"
                     /translation="MGILGGMMKRHEPTLDYRTIDGGHPAPYPDMTDEQLYNYESAFD
                     IRPRTFSEPPRPRAPSAPPAAPERAGAEQTRPVRQKERLRRSVIFDAPRYVARRGAAP
                     AQAATLDFSKPVRTITTKQPVDIITTRARHPVYKVQGYIGDDDVLTVFTMDGRLCENG
                     PVFLENVPEPSRIYLNVYPNGDAASRERFRITQHDSREDADAQAQPGRLACVPLEIVR
                     "
     gene            1033..2793
                     /locus_tag="VM406_01080"
     CDS             1033..2793
                     /locus_tag="VM406_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVL74580.1"
                     /translation="MSTEHLPRSPSPSSSTFADRAAERYRLAVEGSGDGVWDWDIRSD
                     TVYYSPRWKDILGHAEHEIGSSTVEWEHRLHPQDRSAAMAALEACLQGRKAALSSEHR
                     LRCRNGEWKWVLLRARVVERDAGGKPLRMTGTMSDISRRRQSEERVWQHANYDMLTAL
                     PNRRLFRDRLEQEVKKAQRTGWQLALLFIDLDRFKEVNDLLGHDAGDMLLRQAAQRLA
                     GCVRDSDTVARLGGDEFTVILPGIEQMECVGKVADKILHALGEPFRLGNEVAYLSGSV
                     GITIFPDDAREPEELIRNADQAMYGAKHAGRNQFSYFTRSMQERAHARLRLAGDLRGA
                     LAAGQLQVYYQPVVELGSGRIVKAEALLRWHHPELGEVSPSDFIPLAEESGLIMQIGD
                     WVFRQAAACSQRWSERLGHPFQIGVNKSPVQFMAPVGEGDWATYLQQLGLAGSSISVE
                     ITEGLLLNASPLVSERLLAYRDAGIQVAIDDFGTGYSSMAYLKKFDIDYLKIDRSFVR
                     DMTSDDGDRAIAESVIVMAHRLGLKVIAEGIETPEQMELLRAADCDFGQGFLYAQAAP
                     PDEFERLLLEGTGQPGAALH"
     gene            complement(2812..3180)
                     /locus_tag="VM406_01085"
     CDS             complement(2812..3180)
                     /locus_tag="VM406_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HVL74581.1"
                     /translation="MGFGRTTPILRIFDEAKAKAFYLGFLGFRLDWEHRFEPGLPLYI
                     QVSKDACVLHLTEHHGDCCPGAALRIETDELDAFHQQLLAAGYQYARPAVERMPWGSR
                     DMSVTDPFGNRLTFTEAVSA"
     gene            complement(3196..3963)
                     /locus_tag="VM406_01090"
     CDS             complement(3196..3963)
                     /locus_tag="VM406_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL74582.1"
                     /translation="MKSKTLTFFAPWALLLAIIALWQAICTVFEVSEFIFPSPIAIAE
                     ALVEFAGPIAQHAWATFWVTMAGFAIAVAVGLMLGFILGSSPLIYSAAYPLMTAFNAL
                     PKAAFVPILVVWFGIGAGPAILTAFLISFFPIMVNIATGLATLEPELEDVLRVLGAKR
                     RDVLLKVGLPRSMPYFFASLKVAITLAFVGTTVSEMNASNEGIGYLLVSAGSSMKMPL
                     AFAGLVVIGVMAMIMYELFAVIEKRTTAWAHRGAQAH"
     gene            complement(3981..4766)
                     /locus_tag="VM406_01095"
     CDS             complement(3981..4766)
                     /locus_tag="VM406_01095"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL74583.1"
                     /translation="MTDTFVDFKNVFLAYQEGGEFAVEDINLSTRSGEFIAIVGPSGC
                     GKSTFMKLATGLKAPTRGSIAIGGQPVTGPLKIVGMAFQAPTLLPWRTTLDNVLLPLE
                     IVEPYRSNFKRNKAEYVERALALLKKVGLDGYADKFPWQLSGGMQQRASICRALIHEP
                     KMLLLDEPFGALDAFTREELWCMLRDLWSERQFNVILVTHDLREAAFLADTIYVMSKR
                     PGRILARRENPLPQPRDIDVTYTEPFTALVHELREHIGRVRSS"
     gene            complement(4825..5865)
                     /locus_tag="VM406_01100"
     CDS             complement(4825..5865)
                     /locus_tag="VM406_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL74584.1"
                     /translation="MTLNRIPRIARWASGLALVAFSLQASAQETKLKFQLDWRFEGPS
                     AIFLMAQTKGYFAQEKLDVTIDAGNGSGNAVNRVASGTYDMGFADLAALMEFVGNNPT
                     APNKPVGVMMVYNDTPAAVFALKKSGIKTPADLAGKKLGAPVFDAGRRGFPIFAKANK
                     LDPASAQWTSMDPPLRETMLVRGDVDAITGFYFTSLLNLNARGVKDEDIVVLPFSQHG
                     VKLYGNAIIASESFAKQNPEAVKGFLRAIAKSTRDVLANPEEAIKVVKARDGLIDEKL
                     ELRRLKLAIQHSIATQNARAEGFGQISTPRLTLMASQVSDTYGTKNRVDPNTIWNSGF
                     LPSKEELNVFPK"
     gene            complement(6250..6456)
                     /locus_tag="VM406_01105"
     CDS             complement(6250..6456)
                     /locus_tag="VM406_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HVL74585.1"
                     /translation="MLELRVENMTCGGCARHVTQAVQSVDSNARVDVDLAGKKVRVET
                     QADPEAVAAAIAEEGYPATLVRAG"
     gene            6603..7058
                     /locus_tag="VM406_01110"
     CDS             6603..7058
                     /locus_tag="VM406_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HVL74586.1"
                     /translation="MRTYLRFGAMIVTSTVVMYGLMYLNTYSVDHVVFSETRVYMALL
                     MGACMAVIMLTFMLHMHTNRTVNMAIYLGSAAIFFGALWLVRSQSTIDEVSWMRAMIP
                     HHSIAILTSERATISDPRAVRLSKEIIETQRREIAEMKALIQELRSRHQ"
     gene            complement(7152..>8849)
                     /locus_tag="VM406_01115"
     CDS             complement(7152..>8849)
                     /locus_tag="VM406_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HVL74587.1"
                     /translation="AYFYSVAIVFGLRGEDFFWELATLIDVMLLGHWLEMRSILGASG
                     ALEALVKLLPTTAHRLDGQGRLEDVAVSALKVGDRVVVKPGEKVPTDGVIVDGRSSVN
                     EAMLTGESKPVTRGVGDAAIGGAINGEGALTLEIRKTGDATYLAQVIDMVRKAQESRS
                     RTQDIANRAAHWLTWIALLGGGTTFAVWSLIGADLAFSLERMVTVMVVACPHALGLAV
                     PLVVSVSTSQAAGHGLLIRNRAAFEHARKLQAVIFDKTGTLTEGRFGVTDVVALDGGH
                     DERVLQLAASLETASEHPIAHGIVAHARERGLELWAPADFRNFTGEGAQARVDAALVR
                     VVSPGHVRRLGLTYDDSRIAPLLADGKTVVLVLEEQAVIGAIALADIVRPESFEAVQR
                     LKAMGVQCMMLTGDAEIVARTVARQLGLDDYFAEVLPHEKAAKVKEVRARGLVTAMVG
                     DGVNDAPALVESDLGIAIGAGTDVAIESADIVLVRSDPRDVVAITALSRATYRKMIQN
                     LGWATGYNSFALPLAAGVAYTAGFMLTPAFGAALMSISTVVVAINARLLGRQDPAQA"
BASE COUNT         1555 a         2890 c         2861 g         1543 t
ORIGIN      
        1 atcatcgcct caggccaggc tgacatggtc gcgctcgcgc gcggcatcct ctacgatccg
       61 cgctggccct ggcatgcggc tgcgcggctc ggcgcgcagg tcgacgcgcc gccgcaatac
      121 tggcgttctc agccgcgcga ggtgaaggac ctgttccgta agtagtcagc gcacaatctc
      181 caacggcacg caggccagcc ggccgggctg tgcctgcgca tccgcgtctt cccggctgtc
      241 gtgctgggtg atccggaaac gctcgcgaga agccgcatcg ccgttcggat agacgttcag
      301 gtagatgcgg ctcggctccg gcacgttctc gaggaacacc ggcccgtttt cgcacaggcg
      361 gccgtccatg gtgaacacgg tgagcacgtc gtcgtcgccg atgtagccct gcaccttgta
      421 gaccggatgg cgcgcgcggg tggtgatgat gtccaccggc tgcttcgtgg tgatggtgcg
      481 caccggcttg ctgaaatcca gcgtggcggc ctgcgccggt gcggcgccgc ggcgcgcgac
      541 gtagcgcggc gcgtcaaaga tgacactgcg ccgcaagcgc tccttctgac gcaccggccg
      601 ggtttgctcg gcgccggcgc gctcgggcgc cgcaggcggc gccgatggcg cgcgcggcct
      661 cggcggctcg ctgaaggtgc gcggccggat gtcgaaggcg ctttcgtagt tatagagctg
      721 ctcgtccgtc atgtccggat agggagcagg atgcccgccg tcaattgtgc ggtaatcgag
      781 cgtaggctcg tgccgcttca tcatgcctcc cagaatcccc acaggttctc ctcgcgtcct
      841 gtttcaatcg ttgtgcaaac cgtgcgggcg tgcgcatccc tgcaccgttg gcacggccga
      901 tttgaccatg ccggccgggc gccggttcgc tcgctttccg cgtcattgcc ggatatcaaa
      961 atccctcgtg gaacctcttg cattcccgtc gttctatcga tcacagaacc ggggcccaga
     1021 cggccttcat ccatgagcac agaacacctt ccccgttccc ccagcccctc ttcttcgacc
     1081 ttcgccgacc gggccgccga gcgttacagg ctcgcggtgg aaggcagcgg agacggcgtc
     1141 tgggactggg acatccgcag cgacacggtc tactactcgc cgcgctggaa agacatactc
     1201 ggccacgccg agcacgagat cggcagctct actgtcgaat gggagcacag gctgcatccg
     1261 caggaccgca gcgccgcgat ggccgcgctc gaggcctgtc tgcagggtcg caaggcggcg
     1321 ctctcgagcg agcatcggct gcgctgccgc aacggcgaat ggaaatgggt gctgctgcgc
     1381 gcccgggtcg tcgagcgcga cgccggcggc aagccgctgc gcatgaccgg cacgatgagc
     1441 gacatctcgc ggcggcgcca gtcggaggag cgcgtctggc agcatgcgaa ctacgacatg
     1501 ctgaccgccc tgccgaaccg ccgtctgttc cgcgaccggc tcgagcagga agtgaagaag
     1561 gcgcagcgca ccggctggca gctggcactg ctgttcatcg acctggatcg cttcaaggaa
     1621 gtcaacgatc tgctcggcca cgacgccggc gacatgctgc tgcgccaggc cgcgcagcgg
     1681 ctggccggct gcgtgcgcga ctcggacacc gtcgcaaggt tgggcggtga cgaattcacc
     1741 gtgatcctgc ccggcatcga gcagatggag tgcgtgggca aggtggcgga caagatcctg
     1801 cacgcgctgg gcgaaccgtt caggctcggc aacgaagtcg cctacctgtc cggcagcgtc
     1861 ggcatcacga tctttcccga cgacgcgcgc gagcccgagg aactgatccg caacgccgac
     1921 caggcgatgt acggcgccaa gcacgccggg cgcaaccagt tcagctattt cacccgttcg
     1981 atgcaggagc gcgcgcatgc gcggctgcgc ctggccggcg acctgcgcgg cgcgctcgcc
     2041 gccggccagc tgcaggtgta ctaccagccg gtggtggaac tcggcagcgg gcgcatcgtg
     2101 aaggccgagg cactgctgcg ctggcaccat ccggaacttg gtgaggtcag cccgtccgac
     2161 ttcatcccgc tcgccgagga gtccggcctg atcatgcaga tcggcgactg ggtgttccgc
     2221 caggccgcgg catgctcgca gcgctggagc gaacgcctgg ggcatccgtt ccagatcggc
     2281 gtgaacaagt cgccggtgca gttcatggcg ccggtcggcg agggcgactg ggccacgtat
     2341 ctgcagcagc tcggcctggc cggcagcagc atttccgtgg aaatcaccga agggctgctg
     2401 ctcaatgcct cgccactggt ttcggagcgc ctgctcgcct accgcgacgc cggcatccag
     2461 gtcgcgatcg acgacttcgg caccggctat tcatcgatgg cctacctgaa gaagttcgac
     2521 atcgactacc tgaagatcga ccgctcgttt gtgcgcgaca tgaccagcga cgatggcgac
     2581 cgcgcgattg ccgagtcggt gatcgtgatg gcgcaccgcc tggggctgaa ggtcatcgcg
     2641 gagggcatcg agacgcctga gcagatggaa ctgctgcgcg cggccgactg cgatttcggc
     2701 cagggcttcc tgtatgcgca ggcggcgccg ccggatgagt tcgagcgctt gttgctggag
     2761 ggcacgggac aacccggcgc ggcattgcat tgacgcgtgt ttaacgcccg ctcacgcgct
     2821 gaccgcctct gtaaaggtca gtcggttacc gaatggatcg gtgacggaca tgtcgcggct
     2881 gccccacggc atgcgctcca ccgcgggtct cgcgtattgg tagcccgcgg ccagaagctg
     2941 ctggtgaaag gcatcgagtt cgtcggtctc gatccgaagc gccgcgccgg gacagcagtc
     3001 gccatggtgt tccgtcagat gcagcacgca ggcatccttc gatacctgaa tgtacagcgg
     3061 cagtcccggc tcgaagcggt gctcccagtc gagccggaag ccgagaaagc caaggtagaa
     3121 cgccttggct ttcgcttcat cgaagatgcg caggatcggg gtcgtccttc cgaagcccat
     3181 gatcgcgccc cctgttcaat gcgcctgcgc gccgcgatgc gcccacgcgg tcgtgcgctt
     3241 ctcgatcacc gcgaacagct cgtacatgat catcgccatc acgccgatca cgaccagccc
     3301 ggcaaacgcc agcggcatct tcatcgagga accggccgag accagcaggt agccgatgcc
     3361 ttcgttggac gcattcatct ccgacaccgt ggtgccgacg aaggccagcg tgatcgccac
     3421 cttcaacgac gcgaagaagt agggcatcga gcgcggcagg ccgaccttga gcagcacgtc
     3481 gcggcgcttg gcgccgagca cgcgcagcac gtcttccagt tccggctcca gcgtggccag
     3541 gccggtggcg atgttgacca tgatcgggaa gaacgagatc aggaaggcgg tcaggatcgc
     3601 agggcctgcg ccgatgccga accacaccac caggatgggc acgaaggccg ccttcggcag
     3661 cgcgttgaag gcggtcatca gcggataggc ggccgaatag atcagcggcg acgagccgag
     3721 gatgaagccc agcatcaggc ccaccgccac cgcgatcgca aaaccggcca tggtgaccca
     3781 gaacgtcgcc caggcgtgct gggcgatcgg tccggcgaac tcgaccagcg cctcggcgat
     3841 cgcgatcggg ctcgggaaga tgaactcgga cacctcgaac accgtgcaga tcgcctgcca
     3901 cagcgcaatg atggccagca gcagcgccca gggcgcgaag aaagtgagtg tcttgctttt
     3961 catgaatcgt ccttcgtcga tcagctgctg cgcacgcgcc cgatgtgctc gcgcagttcg
     4021 tgcaccaggg cggtgaaggg ttcggtatag gtgacgtcga tgtcgcgcgg ctgcggcagc
     4081 gggttttcgc ggcgcgcaag gatgcgcccg ggacgtttgc tcatcacata gatggtatcg
     4141 gcgaggaagg ctgcttcgcg caagtcatgg gtgaccagga tcacgttgaa ctggcgctcg
     4201 ctccacaggt cgcgcagcat gcaccacagt tcttcgcgcg taaacgcgtc gagcgcgccg
     4261 aagggctcgt cgagcagcag catcttgggc tcatggatca gcgcgcggca gatcgaggcg
     4321 cgttgctgca tgccgccgga cagctgccag ggaaacttgt ccgcatagcc gtcgagtccg
     4381 accttcttca gcagagcgag cgcgcgctcg acgtactcgg ccttgttgcg cttgaagttg
     4441 ctgcggtacg gctcgacgat ttccagcggc agcagcacat tgtccagcgt ggtgcgccat
     4501 ggcagcaggg tcggcgcctg gaaggccatg ccgacgatct tcagcgggcc ggtgacgggc
     4561 tggccgccga tggcgatgct gccgcgtgtc ggcgctttca ggccggtggc cagtttcatg
     4621 aaggtggact tgccgcagcc ggaagggccg acgatggcga tgaactcgcc ggagcgagtc
     4681 gagaggttga tgtcttcgac tgcaaattcg cccccctcct gatatgcgag gaagacgttc
     4741 ttgaagtcga caaaggtgtc ggtcatggcg atgcgcgcga aaaaaagccc tcacccgtgg
     4801 atgagggccc gattcatagg gaaattactt cgggaagacg ttcagctctt ccttggatgg
     4861 caggaagccg ctgttccaga tcgtgttcgg atcgacgcgg ttcttggtgc cgtaggtgtc
     4921 cgagacctgc gaagccatca gcgtcaggcg cggcgtcgaa atctggccga agccttcggc
     4981 gcgtgcattc tgcgtggcga tcgagtgctg gatcgccagc ttcaggcggc gcagttccag
     5041 cttctcgtcg atcaggccgt cgcgcgcctt gaccaccttg atcgcttctt ccggattggc
     5101 cagcacgtcg cgggtcgact tcgcaatcgc gcgcaggaaa cccttcaccg cttccggatt
     5161 ctgcttggcg aagctttccg aggcgatgat cgcgttgccg tacagcttca cgccatgctg
     5221 ggagaacggc aggaccacga tgtcttcatc cttcaccccg cgcgcattca ggttcaggag
     5281 cgaggtgaag tagaaaccgg tgatcgcatc gacatcgccg cgcaccagca tggtttcacg
     5341 cagcggagga tccatgctgg tccattgcgc cgaggccggg tcgagcttgt tggccttggc
     5401 gaagatcgga aagccacggc ggccggcatc gaacaccggg gcgccgagct tcttgccggc
     5461 gaggtcggcc ggcgtcttga tgccggactt cttcaacgcg aacacggcag ccggcgtgtc
     5521 gttgtagacc atcatcacgc cgaccggctt gttcggagcg gtcgggttgt tgccgacgaa
     5581 ttccatcagt gcggcgagat cggcgaagcc catgtcgtag gtgcctgagg ccacgcggtt
     5641 gaccgcattg cccgagccgt tgccggcatc gatcgtcacg tccagcttct cctgcgcgaa
     5701 atagcccttg gtctgggcca tcaggaagat tgccgacggc ccttcgaagc gccagtcgag
     5761 ttggaacttc agcttggtct cctgggccga ggcttgcagg gaaaaggcga ccagcgcaag
     5821 gccggaagcc cagcgcgcga tgcggggaat gcgattcagg gtcatcggga tatctccatg
     5881 agcgggggta aaccggcgtt tggccgggtg cagcctttga ttcttatggc cccgcccttg
     5941 gacaaaggcg gggcacgcgt gcgctgatca tacttgatcg cacaccggac cgggactgcg
     6001 acatatgctg cacagcaata catatgccag gcgccgacgc accggtccag tgcgccgccg
     6061 gctcgattcg gtgcatttct ttccgcatcc gcgtcttgtt ttgcgcactc ccggtgcacc
     6121 cgcagtgcgt ccgcacaagg atgatgcatc ggccaccacg cgtggccgat gtgagcgccg
     6181 cagggccagc gccactccgg attgcgggcg ccggctcccg ccccggtccg gcgctgttgg
     6241 cggaagggcc tacccggccc gcacaagcgt cgccgggtag ccttcctcgg caattgctgc
     6301 ggcgacagcc tcgggatccg cctgcgtctc gacccgcacc ttcttgccgg ccagatcgac
     6361 gtccactcgc gcattgctgt ccaccgactg cacggcctgt gtgacgtggc gcgcgcagcc
     6421 gccgcaagtc atgttttcaa cgcgtagttc aagcatcgcc gctctcctca agaaaattga
     6481 ccgtggtgcc agcttgatgc ttgccaccgt gggaaggtca atggctgcag tgaacgaatt
     6541 tcccgatcct gtgttcctat tgttcacaaa gcagacgtgc gttactccag gaaaggccag
     6601 gcatgcgaac ctatctcaga ttcggcgcaa tgatcgtcac gtcgaccgtt gtcatgtacg
     6661 gattgatgta tctgaacacc tacagcgtgg accatgtcgt ctttagcgaa acccgggtct
     6721 acatggcact tctcatgggt gcctgcatgg cagtcatcat gctcaccttc atgctgcaca
     6781 tgcatacgaa ccgcaccgtc aacatggcga tctacctggg cagcgcggca atatttttcg
     6841 gggcgctatg gctggtgcgc agccagtcca ccatcgacga agtatcgtgg atgcgcgcga
     6901 tgattccgca tcactcgatt gcgatcctga cgagcgagcg agcgacgatt tcagaccctc
     6961 gggcggtaag gctctccaag gagattatcg agacccagcg ccgcgagatc gccgaaatga
     7021 aggcgctcat ccaggagctg cgatcccggc accaatgaca aattgctcga ccagcgagcg
     7081 gtggaacggt catgagcatt caaccggggc gagcgggatg acaagcccga caggtcgtcg
     7141 cagttggaag ctcaagcctg tgccggatcc tgccgcccca gcaaccgggc attgattgcc
     7201 accacgaccg tgctgatcga catcagcgcg gcgccgaaag ccggcgtcag catgaagccc
     7261 gccgtgtaag cgacgccggc ggcgagaggg agcgcgaagc tgttgtaccc cgttgcccag
     7321 cccaggttct ggatcatctt gcgataggtc gcgcgcgaca gtgcggtgat cgccacgacg
     7381 tcgcgcggat cgctgcgcac cagaacgatg tcggccgatt cgatcgccac gtcggttccg
     7441 gcgccgatgg cgatgccgag atccgattcg accagtgcag gcgcatcgtt gacaccatcg
     7501 ccaaccattg ccgtcaccag gccgcgcgcg cgcacctcct tcaccttggc cgctttttca
     7561 tgcggcagca cctcggcaaa gtaatcatcc aggccgagct ggcgcgccac cgtgcgcgcg
     7621 acgatttcgg catccccggt cagcatcatg cactgcacgc ccatggcctt cagccgctgc
     7681 accgcctcga aggattccgg ccgcacgata tcggccaggg caatggctcc gatcaccgcc
     7741 tgttcctcga gcaccagtac cacggtcttg ccgtcggcca gcagcggcgc aatacggctg
     7801 tcatcgtagg tgagcccgag ccggcgcaca tggccggggc tcaccacccg caccagcgca
     7861 gcgtcgacgc gcgcctgcgc gccctcgccg gtaaagttgc ggaagtcggc gggcgcccac
     7921 agctcgagcc ctcgctcgcg tgcatgggcc acgatgccgt gcgcgatcgg gtgttcggac
     7981 gccgtctcaa gcgatgcggc cagttgcagc acgcgctcgt cgtggccgcc gtcgagcgca
     8041 accacatcgg tgacgccgaa gcgcccttct gtcagggtgc cggtcttgtc gaagatcacc
     8101 gcctgcagtt tgcgcgcgtg ctcgaaggcc gcgcgattgc ggatcagcag accatgtccg
     8161 gcggcctgcg acgtgctgac cgacaccacc aatggcaccg ccagtccgag tgcatgcgga
     8221 caggccacca ccatcacggt caccatgcgc tcgagtgaaa acgccaggtc ggcgccgatc
     8281 agcgaccaga cggcaaaggt ggtgccgccg cccagcaagg cgatccaggt cagccagtgt
     8341 gcagcgcggt tggcgatgtc ctgggtgcgc gagcggctct cctgcgcctt gcgcaccatg
     8401 tcgatgacct gggccaggta agtcgcatcg ccggttttgc ggatttcgag cgtgagcgcg
     8461 ccctcgccat tgatcgcgcc gccgatcgcc gcatcgccga cgccccgggt gaccggcttc
     8521 gattcgccgg tcagcatcgc ttcgttcacg ctgctgcgtc cgtcgacaat cacaccgtcg
     8581 gtcggcacct tttcgccggg cttgacgacg acccggtctc cgaccttcag cgcgctcacc
     8641 gccacatcct ccaggcggcc ctgcccatcc agccggtgcg cggtggtcgg caacagcttg
     8701 accaatgcct ccagcgcgcc ggacgcgccc aggatggagc gcatctccag ccagtgcccg
     8761 agcagcatca cgtcgatcag ggtggccagc tcccagaaaa aatcctcgcc gcgcagcccg
     8821 aacacgatgg ccaccgaata gaagtacgc
//
LOCUS       DATLGF010000038        26988 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_128336, whole genome shotgun sequence.
ACCESSION   DATLGF010000038 DATLGF010000000
VERSION     DATLGF010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 26988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26988
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128336"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VM406_01120"
     CDS             complement(<1..554)
                     /locus_tag="VM406_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVL74588.1"
                     /translation="MRKLKLVMVGNGMAGVRTLEELLKLAPDIYEITVFGAEPYANYN
                     RILLSPVLAGEQTIKDIMLNDVDWYAQNGITLHLGKTITQIDRARRQVIADDGTTAEY
                     DRLLLATGSNPFMLPVPGKDLDGVISYRDIQDTNAMIDAAAKHRHAVVIGGGLLGLEA
                     ANGLKLRGMDVTVVHLPNWLMERQL"
     gene            806..2020
                     /locus_tag="VM406_01125"
     CDS             806..2020
                     /locus_tag="VM406_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HVL74589.1"
                     /translation="MKTTSFWKAGHAPTLFASFFYFDLSFMVWVLLGPLAVQIASDLH
                     LTAAQKGLMVATPLLAGALLRIVMGVLVDHLKPKKAGVIGQVVVMAGMLWAWLGSLDS
                     YAHMLTLGVILGVAGSAFAVALPLASRWYPPEHQGTALGIAGAGNSGTAFAALFAPGL
                     ALAFGWQNVFGLALIPLGVAFIVYLAFAKDAPTAPAPKALGEYLAVLKDRDAWWFMFF
                     YSVTFGGFSGLASSLTIYFNSEYGLSPVTAGYFTAACVFAGSLVRPLGGIVADRIGGI
                     KTLSLTYVLAAVFLLLASLRLPTYELALLVFVLAMLALGTGNGAVFQLVPQRFRKEIG
                     VMTGLVGMAGGIGGFYLASSLGYSKQLTGDYQFGLTVFAGLAALALVGLSAVKTRWRT
                     TWGNAAMTTAKI"
     gene            2080..3783
                     /locus_tag="VM406_01130"
     CDS             2080..3783
                     /locus_tag="VM406_01130"
                     /EC_number="2.7.11.-"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-serine/threonine
                     kinase/phosphatase"
                     /protein_id="HVL74590.1"
                     /translation="MTLSVSAGHASATGLRERNEDFVGMVTPKEPELSTKGMIAAVAD
                     GVSGNAGGRDAAEYTVRGLLADYYATPDTWPVTQSLDRVLQAINSWVQRHGTLRSELA
                     GMATTLTTVVLRGGFYYFSHVGDTRLYLMRNGRLARLTTDHVWDRPEMQHVLTRAVGL
                     DTRLAIDHGMGALREGDVLLLVSDGVWAALAEYDLADCARRTAERRCDAQTCADDLIE
                     QALARGSTDNCSAIVLRVDKLPRENLADALTTSRTLPLPPRMKPGQALDGYLVESLIH
                     ASRATLLYRVVESATQRRLVLKTLHPDCADDEQERAAFAHEEWLARRAVARFFPQVVP
                     AENRNWLYYLSTWHEGHTLQQMLDVGTYFTVPEAVGHAERLVRALGALHRRSILHRDI
                     KPANVHLGRDGELRVLDFGVAVSGLDEQQALAAPKAGTPSFLAPELFEGASPSPQSDL
                     YAAGVTLYLMLTRHYPYGEIEPFQHPRFGEPVRPSRYRPDLPLWLENVLLKAVAREPA
                     DRFETAEEFQIALERGASRPLRAPGPVPLAQRNPELLWRSLAAGSLVVNLLLLYLLVM
                     R"
     gene            complement(3923..4732)
                     /locus_tag="VM406_01135"
     CDS             complement(3923..4732)
                     /locus_tag="VM406_01135"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL74591.1"
                     /translation="MDKFNKFIDIQHAEMVFDTKKGRFHALRDINLAVKKGEFITLIG
                     HSGCGKSTLLNLIAGLLDATGGTLLCANREIAGPAPERAVVFQNHSLLPWLTCFENVH
                     LAVERVFGTSETRAQLQERTRAALALVGLSHAQNKRPHEISGGMKQRVGIARALSMEP
                     KVLLMDEPFGALDALTRAHLQDELLKIVAKTQSTVVMVTHDVDEAVLLSDRIVMMTNG
                     PAATIGDIVEVRLPRPRDRVALAHDALYVEYRSAVLEFLYHRQAHPAQEAA"
     gene            complement(4735..5625)
                     /gene="ntrB"
                     /locus_tag="VM406_01140"
     CDS             complement(4735..5625)
                     /gene="ntrB"
                     /locus_tag="VM406_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HVL74592.1"
                     /translation="MENLSRKRAAPAPQVPAELPKAAAQPVAADRPPARAFSLKPFFA
                     AALPPLFGIALLIVVWQIVASRTADFPSPAVTWAHAVELFSDPFYSNGPNDQGIGWNL
                     LSSLRRVATGFGLAALIGIPLGFMIGRFKFLSGMFNPIISLLKPVSPLAWLPIGLLVF
                     KAANPAAIWAIFICSIWPMIINTAVGVQRVPQDYMNVARVLNLSEWKILTKILFPSVL
                     PYMLTGVRLAIGTAWLVIVAAEMLTGGVGIGFWVWDEWNNLNVPHILIAIVVIGVVGL
                     LLEQALVALAKAFTYEDVRS"
     gene            complement(5647..6885)
                     /locus_tag="VM406_01145"
     CDS             complement(5647..6885)
                     /locus_tag="VM406_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HVL74593.1"
                     /translation="MKDSKTNPTRRSLLKAGVAAGALSGLATKGVWAAGSDKPEKEEV
                     KIGFIPLTDCASVVMASVLGFDRKYGIRIVPTKEASWAGVRDKLVNGELDAAHVLYGL
                     IYGVHLGVSGPKKDMAMLMTLNHNGQAITLSKKLADKGAVDGASLAKLMGSEKREYTF
                     AQTFPTGTHAMWLYYWLAANGIDPMRDAKIITVPPPQMVANMRVGNMDGFCVGEPWNH
                     RAIMDNIGVTAITTQDIWKDHPEKVLGTTAEWVNKHPNTARAMTAAVLEASKWIDASL
                     QNRLKMAETVADKSYVNTSVDAINQRILGRYQNGMGRTWDDPNAMKFYNDGYVNMPYL
                     SDGMWFLTQHRRWGLLKEDVDYLAVASQINQIALYREAAALAKVNAPKDTMRTSKLVD
                     GVLWDGKNPKEYAGSFRIKV"
     gene            complement(7141..7764)
                     /locus_tag="VM406_01150"
     CDS             complement(7141..7764)
                     /locus_tag="VM406_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HVL74594.1"
                     /translation="MSDPKQLKILVVNSLVVPDDADEATQIQAERARALRIGLLEGGY
                     NILAALPPGVDLPAQIAELQPDVIIVDEQSDAVLRSVVEATAQERRPIVCFTEDQDPE
                     KMEAAIEAGVSAYVVAGLAAERVKAVLDVALARFRVEQRLRDELNETRIKLAERKLIE
                     RAKGLLMERHRCTEDEAYKKLRRLAMDKNLKLSDVAQRMIDVADLLI"
     gene            7869..8897
                     /gene="tsaD"
                     /locus_tag="VM406_01155"
     CDS             7869..8897
                     /gene="tsaD"
                     /locus_tag="VM406_01155"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HVL74595.1"
                     /translation="MIVLGVESSCDETGLALYDTQRGLLAHALHSQVAMHEEYGGVVP
                     ELASRDHIRRAIPLLEQVLKQGGIGLGDIDAIAYTQGPGLAGALLVGASIACGLGLAL
                     DRPVLGVHHLEGHLLSPLLASEPPEFPFVALLISGGHTQLMRVDGVGRYALLGETLDD
                     AAGEAFDKSAKLLGLGYPGGPAISRLAEFGDPSVYTLPRPMLHSKDLNFSFSGLKTAV
                     LTVVRNQRTNICEQDKADIARAFVDAIVEVLVAKCVTALKQTGLKRLVIAGGVGANRQ
                     LREALNAAAQKRRFRVFYPELEFCTDNGAMIAFAGAMRLMRNPALATKEYGFTVRPRW
                     PLDELEAA"
     gene            complement(8947..9552)
                     /gene="plsY"
                     /locus_tag="VM406_01160"
     CDS             complement(8947..9552)
                     /gene="plsY"
                     /locus_tag="VM406_01160"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HVL74596.1"
                     /translation="MNTLLFALAAYLVGSVSFAVVVSKLYGLSDPRTYGSKNPGATNV
                     LRTGNKAAAALTLLGDGVKGWLAVWLAVQFGPQYGVGDGGIALVALAVFLGHLFPVFF
                     RFVGGKGVATALGVLLGISGWLGLATLATWLLVAYVSRYSSLAALVAAVAAPLYYALF
                     FGMDAIMLAIVVMSGLLIYRHEQNIGKLLSGKESKIGGGKK"
     gene            complement(9560..10051)
                     /gene="ybaK"
                     /locus_tag="VM406_01165"
     CDS             complement(9560..10051)
                     /gene="ybaK"
                     /locus_tag="VM406_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HVL74597.1"
                     /translation="MARKDHVSETPATQFLRKHGIAFTEHPYAYEEHGGTAVSARELG
                     VDEHSVVKTLVMQDEAGKPLIVLMHGDRKVSTKNLARQIGCKSVEPCKPEVANRHSGF
                     LVGGTSPFGTRKAMPVYVEESILSLDKIYINGGRRGYLVGIDPNILTRVLPAKPVHCA
                     LEE"
     gene            10304..10876
                     /gene="phaP"
                     /locus_tag="VM406_01170"
     CDS             10304..10876
                     /gene="phaP"
                     /locus_tag="VM406_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870345.1"
                     /note="Members of this family are phasins (small proteins
                     associated with inclusions such as PHA granules). Note
                     that several different families of phasins have been named
                     PhaP despite very little sequence similarity to each
                     other; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01841 family phasin"
                     /protein_id="HVL74598.1"
                     /translation="MFTVPEQFSGVARANLESQLAVLTALTSKAFENLEKVADLNINA
                     AKASIEDSASVARQLLSARDGQELITVATAQAQPTAAKAIAYTRQLAGIAASAQVELA
                     RAAEEQFAETSRKLAELVDDVAKNAPPGSENVVSIMKTAIGTANAGYEQLSKSTKQAV
                     EVMEANMNTAVSQFAQAAEKTTTRARAAAR"
     gene            complement(10942..12333)
                     /locus_tag="VM406_01175"
     CDS             complement(10942..12333)
                     /locus_tag="VM406_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HVL74599.1"
                     /translation="MKNRALPTPLLSLLAALVLAGCAAAPPIAPVVDVPAQFKEAPAS
                     WKAAEPAEAQARGEWWRAFRDPALDALIEQASTANADLAAAAARVKQARALAGVEAAA
                     RVPQLQAGFGAERAGTGAGVQRNAFTGLIGASYELDLFGRVAASVDAATADADRSEAL
                     HRSVLLALKADVAQTYFRLRGADAERALLQRTAALREENVRINQRRFELGDVGELDVV
                     RARTELALTRSDLHALERQRAQLEHALATLLGRPAPAFALDASPLAQAAFLPAIPAGL
                     PSALLERRPDIAAAQYAMMAAQSRVGVARSALFPAIRLTAAGGVESADLSELLRWSSR
                     SWALGALAALPLLDGGRNRAVIARQQAVLEEAGEQYRGQVLRAFAEVEDNLVGVRTLA
                     SQIDALDEALASARRSVELAESLYRAGRGSYLELLDAQRNLAAAERSAVQLRSARAVS
                     TVALVRALGGGWQ"
     gene            complement(12369..15542)
                     /locus_tag="VM406_01180"
     CDS             complement(12369..15542)
                     /locus_tag="VM406_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HVL74600.1"
                     /translation="MNISRFFIDRPIFAGVLSILIFIAGVLAIFRLPVSEYPEVVPPS
                     VVVRAIYPGANPKVIAETVAAPLEEQINGVENMLYMSSQATSDGSLTLTVTFQVGTDV
                     EQAETQVQNRVQRALPRLPEEVRQIGVVTAKSSPNLTMVVHLWSPDGRYDDVYLRNFA
                     VLNVKDRLARIPGMGEVLLFGAGDYAMRVWLDPQKIAARGMTATDVVAAIREQNVQVA
                     AGVVGAAPSRGSEFQLAVNTRGRLESEEEFGEVVVKTGRDGSVTRLRDVARIELGAND
                     YALRALLNNKSAAAIAVFEAPNANALQLSADVRDAMAELKRTFPEGVEYDVVYDPTQF
                     VRASIKAVVMTLLEAVALVVLVVIVFLQTWRASIIPLAAVPVSIIGTFAVLLLFGFSI
                     NTLSLFGLVLAIGIVVDDAIVVVENVERNIAAGLAPREATVQAMKEVSGPIIAIALVL
                     CAVFVPIAFVSGLSGQFYQQFALTIAISTVISAFNSLTLSPALAAVLLKPHDAPKDAL
                     TRGMDKLFGRFFRWFNAAFGRASVRYEGGVKQVLARKSLSLGVYAVLVVAAVGVFQSV
                     PSGFVPGQDKQYLVGFAQLPDAASMERTDAVIRRMSDIATSVPGVKDSVAFPGLSING
                     FTNAPNAGIVFFGLDDFSARRTPQLSGEAIAQQVNQKLAAIEDAFIMVFPPPPVNGLG
                     TIGGFKMMVEDRGNLGYDALYQAVQALQMKAWQTPELAGVFSSYQINVPQLFADIDRT
                     KARQMGVPLSTIYQTLQINLGSLYVNDFNKFGRTYQVRVQADAPFRSRAEDIAQLKVR
                     SDRGEMIPLSSLMQVRDSYGPDRVQRYNAYVSADINGGPAPGYSSGQAQAAMERIARE
                     VLPPGIAFEWTELTYQEILAGNTMLVIFPLCVLLVFLVLAAQYESWTLPLAIILIVPM
                     SILCALLGVKLTGGDNNVFTQIALFVLVGLASKNAILIVEFARELEAQGKSIVAAALE
                     ASRLRLRPILMTSFAFIMGVVPLVLSSGAGAEMRHAMGVAVFFGMLGVTFFGLFLTPL
                     FYVLLRSLAVRIVRRPAAVAEPQRGAL"
     gene            complement(15628..16833)
                     /locus_tag="VM406_01185"
     CDS             complement(15628..16833)
                     /locus_tag="VM406_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVL74601.1"
                     /translation="MKTLLTLSPAARAAVLTASALAALLLAACGKPVAQELGDAAQAG
                     PPVSAAEVLEKTIVETQEFSGRLEAVERVEVRPRVSGFISSVHFTPGAVVKKGELLFV
                     IDPRPYEAEAMRTEAAAAAARARAELAKVELARAERLVAERAIAQREADEKAASAKEL
                     EAAARAARAAHEAARLNLGFTRVHAPIGGRISKAEVTAGNLVDANVVLTTVVSGDPIY
                     ASFDGDEESWLRVGAMARKGTAVAVRIGLAGESGFPHEGRLEFVDNRIDPATGSVRMR
                     AVLRNPEGALAPGLFARIQLASGNGTGGETRATLISERAVGTDQDRKYVYVVDGEGKA
                     EYRPVTLGPAVDGLRVVRSGLTPGEKIIVNGLQRVRPGAPVTAQLVPMQAEGERAASA
                     PGQSSQPQS"
     gene            complement(16901..17713)
                     /locus_tag="VM406_01190"
     CDS             complement(16901..17713)
                     /locus_tag="VM406_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL74602.1"
                     /translation="MDAELLVRQMPAAGARSAVAARLYAPAGARAAGLVVLFPCGGFE
                     TADLDAVDGLATGLARASGYRVLASTYTTAPVQPFPAAAEDAYAVLAWAMRERAGLGW
                     DGRRLIAAGMEAGGNLAAVAALMARDRGGPRIAGQMLLMPMLDPGLCSESMRQRQAER
                     VTASEVARCVAAYRGYLPHAADRMHPYACPLQVSRLKGLPPALILSVSDDPLRDEAEQ
                     YGAKLAAAGVRTTLARLAPAAAPTLAGVRCDEARSAQMLEHIVQFLAGLDVI"
     gene            17967..19007
                     /locus_tag="VM406_01195"
     CDS             17967..19007
                     /locus_tag="VM406_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014631692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL74603.1"
                     /translation="MDKLQAMEVFVQVVDSGGFTRAAENMQLPKATVSTLVRDLEASL
                     SVKLLHRTTRRVSVTADGAAYYERCLRILADVREAEDALSHTRMSPRGRLRVDVPTAL
                     GRHVLIPALPDFLERYPDIQLDLGCSDRAVDLVEEGVDCAVRAGPLDDLSLIARRVGE
                     LHVVTCAAPSYLALHGRPAHPSELLRHRCLNHFSSRSGKVMEWDFQRGDERLQLKVPG
                     RLALNDSDAYMAALLAGMGIARTMVTSICPYIKAGQLELVLGDWCVEPVPLHVVYPQN
                     RHLSAKVRVFVEWVAQTLAEHPSMQVPEGPCPLHTACISDSYGDGFQPSVPAYSFPTS
                     HVETSVTTTSAR"
     gene            complement(19107..19472)
                     /locus_tag="VM406_01200"
     CDS             complement(19107..19472)
                     /locus_tag="VM406_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HVL74604.1"
                     /translation="MIKVVLNCIGLVAVALGVIGIFLPLVPTTPFLLLASACFLRGSD
                     RLHRWLLNNRWCGKFIRDFEEKRAVPLRAKVTAIALMWISMAFSMTIVPTWWHKAMLL
                     VIAVCVTAYLVRLRTLKEE"
     gene            complement(19469..20377)
                     /gene="xerD"
                     /locus_tag="VM406_01205"
     CDS             complement(19469..20377)
                     /gene="xerD"
                     /locus_tag="VM406_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HVL74605.1"
                     /translation="MNGIAASQAAIDEFCDSLWLEDGLAKNTLEAYRRDMALFADWLH
                     RARGKQLYAADGEDLSAYFATRHEETKATSANRRLSVFKRFFRHALRQNRISTDPCLK
                     LRTAKQPPRFPKTLSEAQVEALLAAPDVSTPLGLRDRTMLELMYASGLRVTELVLLKS
                     IEVGMNEGVLRITGKGSKTRLVPFGEVARAWIERYLKEARPVILNGRSDDALFVTGRG
                     CPMTRQMFWTLIKKHALAAGIDAPLSPHTLRHAFATHLLNHGADLRVVQLLLGHADIS
                     TTQIYTHVARERLKRLHAQHHPRGSA"
     gene            complement(20374..20874)
                     /locus_tag="VM406_01210"
     CDS             complement(20374..20874)
                     /locus_tag="VM406_01210"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HVL74606.1"
                     /translation="MISPAPASVFSAIVKAPFGGVGIRTDAAMLRELVYLPPSFAEAA
                     PADALAERAARQIERYLIEPDFRFDLPLASVGTDFQRRVWAAISAIPRGEVATYGQIA
                     KLIRSAPRAIGQACGANWFPIVIPCHRVTATGGLGGFSNHDDETGFHLGVKRWLLAHE
                     GITAWA"
     gene            complement(20993..21967)
                     /locus_tag="VM406_01215"
     CDS             complement(20993..21967)
                     /locus_tag="VM406_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein BugD"
                     /protein_id="HVL74607.1"
                     /translation="MKIKSIGLIAGLALVASTSAYAQNYPTKTVTMVVPFAAGGPTDT
                     VARLIAQSMSKTLGQQVIVENVGGAGGTIGVARVAKADNDGHTVLLHHIGMSTAPALY
                     RRLSYNPITDFEPIGLVTDVPMTLIARGNFPPKDLKEVISHIKANRDKVTYANAGLGS
                     ASHLCGTLFMSAIGAEMTTVPYKGTGPAMNDLLGGQVDLMCDQTTNTTGQIRAEKVKV
                     YGVTTKTRVPSLPNVPTIHEAGLPNFEVAVWHGVYAPKGTPKVAIDRLTNALQVALED
                     GTVKQRFAELGTEPVAKNRATPDALRAHLKAEIDKWGPVIRAAGAYAD"
     gene            complement(22258..23262)
                     /locus_tag="VM406_01220"
     CDS             complement(22258..23262)
                     /locus_tag="VM406_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HVL74608.1"
                     /translation="MTFKSCILIIAAAFAACFSSLVAAQEQIVIKFSHVVAPETPKGK
                     AALRFKELAEKATNGRVRVDVYPNSQLYKDREELEALQLGAVQMLAPSLAKFGPLGIR
                     EFEVFDLPFIFPNKEALHRVKEGPIGQVLLHKLEAKGIKGLAYWDNGFKVMSANRPLH
                     EPEDFRGLKMRVQPSRVLDAQMRALGANPISLGFGDVYKALQAGVVDGTENPPSNFYT
                     QNMHQLQRHLTVSDHGYLGYAVIVNKKFWDGLPSGIRSALEGAMDEATRYANAIAQKE
                     NDAALEAVRTSGRTIVHVLSEEDKETWRAALEPVHKEMEARIGKALINAVKREIASEY
                     "
     gene            23416..25458
                     /locus_tag="VM406_01225"
     CDS             23416..25458
                     /locus_tag="VM406_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVL74609.1"
                     /translation="MPALLNFAPLARRRRIWQWLVPMLLVLLFLITLLWLPRQAQQME
                     ANDRQEQLIADTLWVEQTIRFQLERIEDGIRLIASEIGTGYLGGQRLRERMTILDRNN
                     RELARLVWFDANGNSVASTDDRALRLEDLPAASRLTAEHARETRTPQYSQPAPPNGLT
                     GPILMDYHFPIFQNNRYLGSLVATYHMSAILDEMVPWWFAQDNEIVLTDADDNVLARR
                     AAGGAGRGVYTHSRDLHLPYANVTLRTNSVKSAPKLLPNLLVGSVIALSIGLIWSLSA
                     LWKDINRRIAAEGALREQVAFRAAMENSLVTGLRARDLEGRITYVNPAFCNMVGMRAE
                     DLLGHKPPMPYWAPEAMNEYQERYAQMLAGTVKPQGHETIFQRADGERIPVLVFESPL
                     VDDSGRQTGWMGSILDISDRKRFEDLNRRQQEKLQASARLATMGEIASTLAHELNQPL
                     AAISSYTTGALNLLDSEAARAGTLDTAMLKGALEKANAQAQRAGHIIRSVHTFVKRRE
                     PSREKVDLSALIDSVIPLVELQARQFFVVIQTEVEDKLPPVLADPVMLEQVLLNLTRN
                     GIEAMQAVPPPRRRLRITAGLDRQSPVSVVVSVTDQGHGIPDEVAERLFSPFFSTKAE
                     GMGMGLNICRTTIEFHGGKLSYAANPAGGTIFRFSLPVSATAGIETVAAAPVISGK"
     gene            25539..26129
                     /locus_tag="VM406_01230"
     CDS             25539..26129
                     /locus_tag="VM406_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL74610.1"
                     /translation="MLHIVDDEEVIRDSLSWLAQSRSIPTAVYDSGAAFLAALGPDKA
                     FDQEGECVLLDVRMPDMSGAVLFDTLANRGLIQRLPVIFLTGHGDVPMAVDSLKRGAF
                     DFFEKPFNDNKLMDRVREALAASRKALDMAEVHARLANLSAREREVLDLILAGKMNKV
                     IADELGISMRTVEVHRAHIFDKMSVKTAVELARLLK"
     gene            26205..>26988
                     /locus_tag="VM406_01235"
     CDS             26205..>26988
                     /locus_tag="VM406_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HVL74611.1"
                     /translation="MSIATILFPDFALILLGFLLMRYADWGNGFWSGLEKLIYYFLFP
                     ALLFNTTSRTEFDFASTGVMLQVAVIGILSAIALGWLAKPLFRAPPMMFESGVQTAFR
                     FNSYIALAVASRLAGDKGTTLMALIIGFAVPLCNMAAVHALARKNGRLLRELARNPLL
                     LATASGMAFNLLGLELPEMVSATLGRMGNASIALGLIAVGAGLRLSGVEAGKDMAAYF
                     IAVKLLAKPAIALLLGMALQLPPPQLQIVVAFCAMPTASSAYV"
BASE COUNT         4672 a         9026 c         8613 g         4677 t
ORIGIN      
        1 agctggcgct ccatcagcca gttcggcagg tgcacgacgg tcacgtccat gccgcgcagc
       61 ttcaggccat tggcggcttc cagcccgagc aggccgccgc cgatcacgac cgcgtgcctg
      121 tgtttcgccg cggcgtcgat catcgcgttc gtgtcctgga tgtcgcgata ggaaatgacg
      181 ccgtcgagat ccttgccggg caccgggagc atgaagggat tggagccggt tgccagcagc
      241 aggcgatcgt attcggccgt ggtgccatcg tcggcgatca cctggcgtct tgcgcgatcg
      301 atctgcgtga tcgtcttgcc gagatggagt gtgatgccat tctgcgcata ccagtcgaca
      361 tcattgagca tgatgtcctt gatggtctgc tcgccggcca gcaccggcga gagcaggatg
      421 cggttgtaat tcgcatacgg ctcggcgccg aacacagtga tctcataaat gtcgggagca
      481 agtttcagca attcttccag cgtgcgaacg ccggccatgc cgttcccgac cattaccaac
      541 ttcagcttcc tcattaaagc tccccttgct gacggcctcg ccattgaaac cattcgagcg
      601 cgggctgatg caaaacgagt gccagtggtt ttctcatgaa aatgcactgc gttggcgtga
      661 tttcgcacta gcgcagtgca cgagaaggcg cgcacatggt gcgacgcacg ttgcgaaggc
      721 acgaacatgc gctgttttcg cgacaaatcc gtggcacacc gattgcatcc ctgcacggca
      781 attccatcgg atgcaaggga gcatcgtgaa aacaacgtcg ttctggaagg cgggtcatgc
      841 accgaccctg ttcgcctcgt ttttttattt cgatctgagc ttcatggtct gggtgctgct
      901 ggggccgctg gccgtgcaga tcgcatcgga cctgcatctg acggctgcgc agaaagggct
      961 gatggtcgcc acgccgcttc tggccggcgc attgctgcgc attgtgatgg gcgtgctggt
     1021 cgaccacctg aagccgaaga aagccggcgt gatcggccag gtggtcgtga tggcaggcat
     1081 gctttgggcg tggctgggca gcctcgacag ctatgcgcac atgctcacgc tcggcgtgat
     1141 cctcggcgtg gccggctcgg cgttcgcggt tgcgctgccg ctcgcctcgc gctggtatcc
     1201 gcccgaacac cagggcacgg cgctgggcat tgccggcgcg gggaactcgg gcacggcctt
     1261 cgccgcgctg ttcgcgcctg gccttgcgct cgcgttcggc tggcagaacg tgttcgggct
     1321 ggcgctgatt ccgcttggcg tcgccttcat cgtttacctg gccttcgcca aggatgcgcc
     1381 gaccgcgccc gcgcccaagg ccctcggcga gtacctcgca gtgctcaagg acagggatgc
     1441 gtggtggttc atgttcttct acagcgtcac cttcggcggg ttttccggcc tcgcctcttc
     1501 gctgaccatc tatttcaaca gcgagtacgg gctgtcgccc gtcacggccg gctacttcac
     1561 tgcggcctgc gtattcgccg gctcgctggt gcgtccgctc ggcggcatcg tggccgaccg
     1621 catcggcggc atcaagacgc tgtccttgac gtatgtgctg gcggcggtgt tcctgctgct
     1681 ggccagcctg cggcttccca cctacgagct ggcgctgctg gttttcgtgc tggcgatgct
     1741 ggcattgggc accggaaacg gcgccgtgtt ccagctggtg ccgcagcgct ttcgcaagga
     1801 aatcggcgtg atgaccggcc tggtcggcat ggccggcggc atcggcggct tctatcttgc
     1861 gtccagcctc ggctattcga agcagctcac cggcgactac cagttcggcc tgaccgtgtt
     1921 cgccggactg gcggcactgg cgctcgtcgg cctctccgcg gtgaagacgc gctggcgcac
     1981 gacctggggc aatgcggcga tgacgaccgc caagatctga acgtagaatc gctggaggcc
     2041 aattccatgg gcgtggccct tccctccacg cgtcccgaca tgaccctctc cgtcagcgcc
     2101 ggccatgcgt cggcaaccgg cttgcgcgaa cgtaacgagg atttcgtcgg catggtcacg
     2161 ccgaaggagc cggaactcag caccaagggc atgatcgcgg ccgtcgccga cggcgtctcc
     2221 ggcaatgccg gcgggcgcga tgcggccgaa tacacggtgc gcggattgct ggccgactac
     2281 tacgccacgc cggacacctg gccggtcacg cagtcgctgg accgcgtgct gcaggcgatc
     2341 aatagctggg tacaacgaca cggcacgctg cgcagcgaac tggccggcat ggccaccacg
     2401 ctgaccaccg tcgtgctgcg cggcggcttc tactattttt cccacgtcgg cgacacccgg
     2461 ctttacctga tgcgcaatgg ccggctcgcg cgcctgacca ccgatcatgt ctgggaccgc
     2521 ccggaaatgc agcatgtgct cacgcgtgcg gtcggtctcg atacgcgcct ggcgatcgac
     2581 cacggcatgg gcgcgctgcg cgagggcgac gtgctgctgc tggtgtccga cggcgtctgg
     2641 gccgcgcttg ccgaatacga tctagccgat tgcgccagac gcaccgccga gcgccgctgc
     2701 gatgcccaga cgtgtgcgga cgacctgatc gagcaggcgc ttgcccgcgg ctcgaccgac
     2761 aattgcagcg cgatcgtcct gcgcgttgac aagctgccac gggaaaacct cgccgatgcg
     2821 ctgaccactt cccgcacgct gccgctgccg ccgcggatga agccgggaca ggcgctcgac
     2881 ggctacctcg tcgaaagcct gatccacgcg tcgcgcgcga cgctgctgta ccgtgtcgtc
     2941 gagtcggcca cgcagcgtcg gttggtgctc aagacgctgc atccggactg cgcggacgat
     3001 gaacaggagc gcgccgcgtt cgcccacgag gagtggctcg cacggcgcgc ggtcgcacgc
     3061 ttctttccgc aggtggtccc tgccgagaac cgcaactggc tgtactacct gagcacctgg
     3121 cacgaaggcc atacgctgca acagatgctc gatgtcggca cctacttcac ggtgccggag
     3181 gcggtcggcc atgccgagcg gctggtgcgc gcgctcggcg cgctgcaccg gcgcagcatc
     3241 ctgcatcggg acatcaagcc cgccaacgtc cacctcggca gggacggcga gctgagggtg
     3301 ctcgatttcg gcgtggccgt ctccggcctg gacgagcagc aggcgctcgc cgcgcccaaa
     3361 gccggtacgc cgtccttcct tgcgccggag ctgttcgaag gcgcgtcgcc ctcgccgcaa
     3421 agcgacctgt acgcggccgg cgtgacccta tacctgatgc tgacccgcca ctacccgtat
     3481 ggcgaaatcg aacccttcca gcatccgcgc ttcggcgaac cggtgcggcc gagccggtac
     3541 aggccggacc tgccgttgtg gctggagaat gtcttgctga aggccgtcgc ccgcgagccc
     3601 gcggatcgct ttgagaccgc cgaagagttc cagatcgcgc tggagcgtgg tgcgagccgg
     3661 ccgttgcgcg cgccggggcc ggttccgctc gcgcagcgca atccggaatt gctgtggcgc
     3721 tcgctcgcgg caggctcgct ggtggtgaac ctgctgttgc tgtatctgct ggtgatgcgg
     3781 tagctgtgca ggtattgagc agggcgaacg gatgaggccc ctcactgcgt tcgggacgac
     3841 aagagtacgc cctgtcgttc cgaaagcagt gaggaacctc atccatctgc aacgtgaagg
     3901 cgcccgcacc acgcgcgcgt cgctacgccg cctcctgcgc cggatgcgcc tgccggtgat
     3961 acaggaactc cagcaccgcg ctgcggtact cgacatagag cgcatcatgc gcgagcgcca
     4021 cccggtcgcg cgggcgcggc agccggacct cgacgatatc gccgatcgtg gccgcaggcc
     4081 cgttggtcat catcacgatc cggtccgaca gcagcaccgc ctcatccacg tcgtgcgtga
     4141 ccatcaccac ggtcgactgc gtcttggcca cgatcttcag gagttcgtcc tgcaggtgcg
     4201 cgcgcgtgag cgcatcgagc gcgccgaacg gctcgtccat cagcagcacc ttcggctcca
     4261 tcgacagcgc ccgcgcgatg ccgacacgct gtttcatccc accggaaatt tcatgcggac
     4321 gcttgttctg cgcgtgcgac agccccacca gagcgagcgc ggcgcgcgtg cgctcttgga
     4381 gttgcgcgcg cgtttcgctt gtcccgaaca ctcgctccac cgccagatgg acattctcga
     4441 aacaggtcag ccacggcagc agcgaatggt tctggaagac gaccgcccgc tccggcgccg
     4501 gaccggcgat ttcgcggttg gcgcacaaca gcgtgccccc ggtcgcatcg agcaggccgg
     4561 cgatcaggtt cagcagcgtc gacttgccgc agcccgagtg gccgatcagc gtgatgaact
     4621 cccccttctt cacggccagg ttgatgtcgc gcagcgcgtg gaaacggcct ttcttcgtat
     4681 cgaacaccat ttcggcatgc tggatgtcga tgaacttgtt gaacttatcc atgatcagct
     4741 ccgcacgtct tcataggtga aggcctttgc cagcgccacc agcgcctgct ccagcaacag
     4801 cccgaccacg ccgatcacca cgatggcgat caggatgtga ggcacgttca ggttgttcca
     4861 ttcgtcccag acccagaagc cgattccgac gccgccggtc agcatctcgg ccgcgacgat
     4921 caccagccag gccgtgccga tcgccagccg cacgccggtc agcatgtacg gcagcaccga
     4981 cgggaacagg atcttcgtga ggatcttcca ctccgacagg ttcagcacgc gcgcgacgtt
     5041 catgtagtcc tgcggcacgc gctgcacgcc aaccgcggtg ttgatgatca tcggccagat
     5101 cgagcagatg aagattgccc agatcgcagc cgggttcgcg gccttgaata ccagaaggcc
     5161 gatcggcagc caggccagcg gcgataccgg tttcaacagg ctgatgatcg gattgaacat
     5221 ccccgacagg aacttgaagc ggccgatcat gaagccgagc ggaatgccga tcagcgcagc
     5281 cagcccgaag ccggttgcga cacggcgcag cgaggacagc aggttccagc ctatgccctg
     5341 gtcgttcggg ccgttgctgt agaacggatc ggagaacagt tcgaccgcgt gcgcccaggt
     5401 caccgcaggc gacggaaaat cggctgtgcg cgacgcgacg atctgccaga ccacgatcaa
     5461 cagcgcaatg ccgaacagcg gcggcagcgc ggcagcaaag aatggcttga gcgaaaacgc
     5521 acgcgccggg ggtcgatctg ccgccaccgg ctgcgcggca gccttgggca attcggcggg
     5581 gacttgcggc gcgggagcgg cccgcttgcg ggaaaggttt tccatgactg cactcatcgc
     5641 cgcacctcag accttgatcc tgaacgagcc cgcatactcc ttcgggttct tgccgtccca
     5701 gagtacgccg tcgaccagct tcgaggtgcg catcgtgtcc ttgggcgcgt tcaccttcgc
     5761 cagcgccgcc gcttcgcggt acagcgcgat ctggttgatc tgggaggcca cggcgagata
     5821 atcgacgtct tccttcagca atccccagcg gcggtgctgc gtcaggaacc acatgccgtc
     5881 ggacagatac ggcatgttca cgtagccgtc gttgtagaac ttcatcgcgt tcggatcgtc
     5941 ccaggtcctg cccatgccgt tctggtagcg gccgaggatg cgctggttga tcgcgtcgac
     6001 actggtgttc acgtatgact tgtcggcgac ggtttccgcc attttcaggc ggttctgcag
     6061 gctcgcatcg atccatttgc tcgcctccag cacggcggcg gtcatcgcgc gcgccgtgtt
     6121 cggatgcttg ttcacccact ccgcggtggt gccgagcacc ttctccggat gatccttcca
     6181 gatgtcctgc gtggtgatcg cggtgacgcc gatgttgtcc atgatcgcgc gatggttcca
     6241 cggctcgccg acgcagaagc cgtccatgtt cccgacgcgc atgttcgcca ccatctgcgg
     6301 cggcggcacg gtgatgatct tggcgtctct catcgggtcg atgccgttgg ccgcgagcca
     6361 gtagtacagc cacatcgcgt gcgtgccggt cgggaaggtc tgggcgaacg tgtattcgcg
     6421 cttctcgctg cccatcagct tcgccagcga tgcgccgtcg accgcgccct tgtcggccag
     6481 cttcttcgac agcgtgatcg cctgcccgtt atggttcagc gtcatcagca tcgccatgtc
     6541 ctttttcgga ccggagacgc ccaggtgcac gccgtagatc aggccataga gcacatgggc
     6601 ggcgtcgagc tcgccgttga ccagcttgtc gcgcacgccg gcccaggacg cttccttggt
     6661 cggcacgatc ctgatgccgt acttcctgtc gaagccgagc accgaggcca tcaccaccga
     6721 cgcgcagtcg gtcaggggaa tgaaaccaat cttcacctcc tccttttccg gcttgtcgga
     6781 accggccgcc cacacgcctt ttgtcgcgag gccggacaag gcgcccgcgg cgacgccggc
     6841 cttcagcaag ctgcggcgag tcggattggt tttgctgtct ttcatgaatc ccccagtgga
     6901 tatggccgaa ataaaaaagg cgtacgtgcc tggcgggtgg atacccggtc agcacggacg
     6961 cctttgtcca aactactccg gccctcgctg gccggagcag aaatctgttg cgccatcgtt
     7021 ggcgcgcatc aagtattgca agcgttgtgc cagcgcctga tgcgcggcgg tggagcaaaa
     7081 atcaggggga atcgggattc acggcgcacc gcgaacgtgc ggtgcgtcaa cctggggcac
     7141 tcaaatgagg agatcggcga cgtcgatcat tctctgggcg acgtcggaca gcttcagatt
     7201 cttgtccatc gcgagccgcc gcagcttctt gtaggcctcg tcctcggtgc agcggtgccg
     7261 ttccatcaac aggccctttg cgcgctcgat cagcttgcgc tcggccagct tgatgcgggt
     7321 ttcgttgagc tcgtcgcgca gtctctgctc gaccctgaag cgcgcaagcg cgacgtcgag
     7381 cacggccttc acgcgctcgg ccgcgaggcc ggccacgacg taggccgata ccccagcctc
     7441 gatcgccgcc tccattttct ccgggtcctg gtcctcggtg aagcagacga tgggccggcg
     7501 ttcctgcgcg gtcgcttcca ccacgcttct cagcaccgcg tcggattgtt cgtcgacgat
     7561 gatgacgtcc ggctgcagtt cggcgatctg ggcgggcagg tccacacctg gcggcagcgc
     7621 ggccaggatg ttgtagccgc cttcgagcag gccgatgcgc agcgcgcgcg cacgctcggc
     7681 ctggatctgg gtggcttcgt cggcgtcgtc gggcactacc agggaattga cgaccaggat
     7741 cttcagctgc ttcgggtcac tcatgcttgg ctcttgtgat caatacggaa aggctccctt
     7801 cgtatcgaat acgactccgc cctttccttt agaatggcgc attgcaagaa ccctgccatc
     7861 tccccttcat gattgtcctc ggcgtcgaat cctcctgcga cgaaaccggc ctcgctttgt
     7921 atgacacgca acgcgggctg cttgcgcatg cgctgcattc gcaggtcgcg atgcacgaag
     7981 agtacggcgg cgtcgtgccc gagctcgcgt cgcgcgacca tatccggcgg gcgattccgc
     8041 tgctcgaaca ggtgttgaag caggggggga tcgggctcgg cgacatcgac gcgatcgcct
     8101 acacgcaggg cccgggcctg gccggcgcgc tgctggtcgg cgcctcgatc gcctgcggac
     8161 tgggattggc gctcgatcgc ccggtgctgg gcgtgcacca cctggagggc cacctgctgt
     8221 cgccgctctt ggccagcgag ccaccggaat tcccgttcgt cgcgctgctg atttcaggcg
     8281 gccatacgca gttgatgcgc gtagacggcg tcggccgtta tgcgctgctc ggtgaaaccc
     8341 tcgacgatgc ggccggtgag gcgttcgaca agtcggccaa gctgctcggg ctgggctacc
     8401 cgggcggccc tgcgatctcg cgactggccg agttcggcga cccttccgtc tacacgctgc
     8461 cgcggccgat gctgcattcg aaggatctga acttcagctt ttccggcctg aagaccgcgg
     8521 tgctcaccgt cgtgcgcaac cagcgcacga acatttgcga acaggacaag gcggacattg
     8581 cgcgcgcctt cgtcgacgcg atcgtcgaag tgctggtcgc caagtgcgtg acggcgctga
     8641 agcagaccgg cctgaagcgc ctggtgattg ccggcggcgt cggcgcgaac cgccagttgc
     8701 gcgaggcgct caacgccgcc gcgcagaagc ggcgcttccg cgtcttttac ccggaactgg
     8761 agttctgcac cgacaacggt gcgatgatcg ccttcgccgg tgcgatgcgg ctgatgcgca
     8821 atccggcgct ggcgacgaag gagtacggct tcaccgtgcg gccgcgctgg ccgctcgacg
     8881 aactggaagc ggcatagagc tacgtagatc ggctggactc gcgtcgcgtt ggcgacggtg
     8941 tcccgcctat ttcttcccgc cgccgatctt gctttccttg cccgacagga gcttgccgat
     9001 gttctgctcg tggcggtaga tcaacagccc actcatcacg acgatcgcca gcatgatcgc
     9061 gtccatcccg aagaacagcg cgtaatacag cggagccgca accgccgcga ccagcgcggc
     9121 cagcgaggag taacggctca cgtacgcgac cagcagccag gtcgccaatg tggcaagccc
     9181 gagccagccg gagatgccga gcaggacgcc gagcgcggtc gccacgccct tgccaccgac
     9241 gaagcgaaag aacaccggga acaggtggcc gaggaagacc gccagcgcca ccagcgcgat
     9301 gccgccatcg ccgaccccgt attgcggacc gaactggact gcgagccaga cggccagcca
     9361 gcccttgacc ccgtcgccca gcaaggtcag cgcggcggcg gccttgttcc cggtgcgcag
     9421 cacattggtc gcgccgggat tcttcgaccc gtatgtccgc ggatccgaca gaccgtagag
     9481 cttgctcacc accacggcaa acgaaaccga accgaccagg taggcggcca gcgcaaagag
     9541 cagggtattc atgatgtcct tattcctcca gcgcgcaatg caccggcttg gccggcagta
     9601 cccgggtcag gatattggga tcgatcccga ccagataacc acgccggcct ccattgatat
     9661 agatcttgtc gagcgacaag atgctctcct ccacgtacac cggcatggcc ttgcgcgttc
     9721 cgaacggcga cgttccgccg accagaaagc ccgagtggcg gtttgctact tccggcttgc
     9781 agggctccac cgacttgcag ccgatctggc gcgccaggtt cttcgtcgag accttgcgat
     9841 cgccgtgcat cagcacgatc agcggcttgc ccgcctcgtc ctgcatgacc agcgtcttca
     9901 cgacgctgtg ttcgtcgacg ccgagttcgc gcgccgaaac ggccgtgccc ccgtgctcct
     9961 cataagcgta gggatgctcg gtgaacgcga tcccgtgctt gcgcaggaac tgggtcgcgg
    10021 gagtttcgga aacgtgatct ttcctggcca tcgtgacggt gcgaaaaagt gccgcacatt
    10081 atgcagcaag agcacgaagc aaagcagatc tccgttgctc ctgggaaaaa cgaactgtgc
    10141 gaaatcaata tagggcgagc ccgatgctgc caaaatcgcc ggtgcgggcg cgaatttgtg
    10201 cgccgcacaa aaatcttctt gacatgtcgg aacaggcgca taaaataggt tttgttgcag
    10261 cgcacaatgc tgacatgacc aacttctgac aaggagaaac acaatgttca ccgtacccga
    10321 gcaattttcc ggcgttgccc gcgccaacct cgaatcccaa ctcgcagtcc tgaccgcgct
    10381 gaccagcaag gccttcgaaa acctggaaaa agtggctgac ctgaacatca acgccgccaa
    10441 ggcgtcgatc gaggattctg cctctgtcgc ccgtcagctg ctgtcggcca gggacggcca
    10501 ggaactgatc accgttgcga ctgcccaggc tcagcccacc gcggccaagg cgatcgccta
    10561 cacccgccag ctggccggca tcgccgccag cgcgcaagtc gagctggccc gcgccgccga
    10621 agagcagttc gccgaaacca gccgcaagct ggctgaactg gtcgacgatg tggcgaagaa
    10681 cgccccgccc ggttcggaga acgtggtctc gatcatgaag actgcgatcg gcaccgcaaa
    10741 cgccggctac gagcagctga gcaagtcgac caagcaggcc gtcgaagtca tggaagcgaa
    10801 catgaacacc gccgtcagcc agttcgccca ggcggccgag aagaccacga cgcgtgcccg
    10861 cgccgccgcg cggtaatcca gccgcacaaa aaacaaagcc ccgcacggga aaccctgcgg
    10921 ggctttttca tgggctgcga tttactgcca tccaccgccc agtgcccgca ccagggccac
    10981 cgtcgacacc gcgcgggcgc tgcgcaactg caccgcactg cgctcggcgg cggcaagatt
    11041 gcgctgggcg tcgagcaact ccaggtagct gccgcgtccg gcccggtaga ggctctcggc
    11101 aagttcgacc gagcgtcttg cggaagcgag cgcctcgtcc agcgcatcga tctgcgacgc
    11161 gagcgtgcgc acgccgacca gattgtcttc gacctcggca aaggcccgca gcacctggcc
    11221 gcgatactgc tcccccgcct cctccaggac cgcctgctgc cgcgcgatga cagcacggtt
    11281 gcgccccccg tccagcagtg gcagcgccgc cagcgcgcca agtgcccagc tgcggctgct
    11341 ccagcgcaac agttccgaca gatcggccga ctccaccccg ccggcggcgg tcagccggat
    11401 cgccgggaac agcgcgctgc gcgcaacgcc gacgcgggac tgtgccgcca tcatcgcgta
    11461 ctgggcggcg gcaatgtctg gccggcgctc cagcagggcc gacggcaatc cggccgggat
    11521 agcgggcaga aaggcggcct gggcgagcgg actcgcgtcg agcgcaaacg ccggcgccgg
    11581 ccggcccagc aacgtggcca gcgcatgctc tagctgggcg cgctggcgct ccaaggcatg
    11641 caggtccgaa cgggtcagcg ccagctcggt gcgcgcgcgc accacgtcca gctcgccgac
    11701 atcgccgagc tcgaagcggc gctgattgat gcgcacgttc tcctcgcgca gcgcggcggt
    11761 acgttgcagc agcgcgcgct cggcatcggc gccgcgcagg cggaaatagg tctgcgcgac
    11821 atcggccttt agcgccaaca gcacggagcg atgcagcgcc tcgctgcggt cggcatcggc
    11881 cgtggccgcg tccacgctgg ccgcaacccg gccgaacaga tcgagctcgt agcttgcgcc
    11941 gatcaagccg gtaaatgcat tgcgctgcac gcctgcgcct gtcccggcgc gctccgcgcc
    12001 gaacccggcc tgcagctgcg gcacgcgggc ggcagcctcc acgccggcca gcgcccgcgc
    12061 ctgcttcacc cgcgcggcgg cggcggcgag atccgcgttg gcggtgctcg cctgctcaat
    12121 cagcgcatcg agggccgggt cccggaacgc cctccaccac tcgccgcgcg cctgcgcctc
    12181 ggccggttcc gcagccttcc acgatgcggg cgcttccttg aattgcgccg gcacatccac
    12241 aaccggcgcg atcggcggcg cagcggcgca gccggccagc acgagggcgg ccagcagcga
    12301 cagcaagggt gttggtaatg cacggttttt catagtcttg cttccttggt cattgaagtc
    12361 ggcttgcatc agagcgctcc tcgttgcggt tcggccactg cagccggccg gcgtacgatg
    12421 cgcaccgcga ggctgcgcag cagcacgtag aacaagggcg tgaggaacag gccgaagaag
    12481 gtgacgccga gcatgccgaa gaacacggcc acgcccatcg catggcgcat ctcggcgccc
    12541 gcccccgagg acagcaccag cggcaccacg cccatgatga aagcgaacga agtcatcaga
    12601 atcggacgca gccgcaggcg gctggcttcc agcgccgcag ccacgatcga tttgccctgc
    12661 gcctcgagct cgcgcgcgaa ctcgacgatc aggatcgcgt tcttcgaggc caggccgacc
    12721 agcacgaaca aggcgatctg cgtgaacaca ttgttgtcgc cgccggtcag cttcacgccc
    12781 agcagcgcac acaggatcga catcggcacg atcaggatga tggccagcgg cagggtccag
    12841 ctctcgtact gcgcagccag caccaggaac accagcaaca cgcacagcgg gaagatgacg
    12901 agcatggtgt tgccggccag gatctcctga taggtcagct cggtccattc gaacgcaatt
    12961 cccggcggca gcacttcgcg cgcgatgcgc tccatcgccg cctgcgcctg gccgctcgaa
    13021 tagccgggcg cggggccgcc gttgatgtcg gccgacacat aggcgttgta gcgctgcacg
    13081 cgatccgggc cgtagctgtc gcgcacctgc atcagcgaag acagcggaat catttcaccg
    13141 cggtcgctgc gcaccttcaa ctgggcgatg tcttcggcgc gcgagcgaaa cggcgcatcg
    13201 gcctgcaccc ggacctggta ggtgcgcccg aacttgttga agtcattgac gtacagcgag
    13261 ccgagattga tttgaagggt ctgatagatc gtcgacagcg gcacgcccat ctgcctggcc
    13321 ttggtgcggt cgatgtcggc gaacagctgc ggcacgttga tctggtagct ggagaacacg
    13381 ccggccagtt ccggcgtctg ccaggccttc atctgcaagg cctgcaccgc ctggtacagc
    13441 gcgtcatagc cgagattgcc gcggtcctct accatcatct tgaagccgcc gattgtgccg
    13501 agcccattca ccgggggcgg cggaaaaacc atgatgaagg catcctcgat cgccgccagc
    13561 ttctggttga cctgctgcgc gatcgcctct cccgacagtt gcggcgtgcg gcgcgcgctg
    13621 aaatcgtcca ggccgaagaa gacgattccg gcgttcggcg cattcgtgaa accgttgatc
    13681 gagagccccg gaaacgcgac ggaatccttc acccccggta ccgaggtggc gatgtcggac
    13741 atgcggcgga tcaccgcgtc ggtccgctcc atcgaagcgg catcgggcag ctgcgcgaag
    13801 ccgaccaggt actgcttgtc ctgccccggc acgaagccgc tcggcaccga ctggaacacg
    13861 ccgaccgcgg ccacaaccag caccgcatac acgccgagcg agaggctctt gcgcgccagc
    13921 acctgcttga cgccgccttc gtagcgcacc gaggcccgcc cgaatgccgc attgaaccag
    13981 cggaagaaac ggccgaacag cttgtccatg ccgcgcgtga gcgcatcctt gggcgcatcg
    14041 tgcggcttga gcagcaccgc ggccagcgcc ggcgacagag tcagcgaatt gaacgcggag
    14101 atcacggtgg agatcgcaat cgtcagcgca aactgctgat agaactggcc ggacaggcca
    14161 gatacgaacg cgatcggcac gaacaccgcg cacaggacca gcgcaatcgc gatgatcggc
    14221 ccgctgactt ccttcatcgc ctgcacggtg gcctcgcgcg gcgccagtcc ggccgcgatg
    14281 ttgcgctcga cgttctccac gaccacgatc gcgtcgtcga ccacgatgcc gatcgccagc
    14341 acgaggccga acagggacag cgtattgatc gagaagccga acagcagcag cacggcaaac
    14401 gtcccgatga tggaaaccgg cacggcggcc agcggaatga tcgaggcgcg ccaggtttgc
    14461 aggaacacga tcacgaccag caccaccagg gccaccgcct caagcaaggt catcaccacg
    14521 gccttgatcg aggcccgcac gaactgggtc gggtcgtaca cgacgtcgta ctccacgcct
    14581 tcggggaatg tccgcttcag ctcggccatc gcgtcgcgca cgtccgccga cagctgcaag
    14641 gcattcgcgt tcggcgcttc gaagacggcg atcgccgccg cactcttgtt gttcagcagc
    14701 gcgcgcagcg catagtcgtt cgcccccagc tcgatgcgcg ccacgtcgcg caggcgtgtg
    14761 accgagccgt cgcggccggt cttgacgacg acctcgccga actcctcttc gctttccagc
    14821 cgcccgcgcg tgttcaccgc aagctggaat tcgctgccgc gcgaaggcgc tgcgccaacc
    14881 acgccggcgg ccacctgcac gttctgctcg cggatggcgg cgaccacgtc ggtcgccgtc
    14941 atgccgcgcg ccgcaatctt ctgcggatcc agccatacgc gcattgcgta gtcgcctgcg
    15001 ccgaacagca gcacctcgcc catgccgggg atgcgcgcga gcctgtcctt cacattgagc
    15061 acggcgaagt tgcgcaggta gacgtcgtca tagcgaccgt ccggcgacca tagatgcacg
    15121 accatcgtca ggttcggcga gctcttggcg gtgaccacgc cgatctggcg cacctcctcg
    15181 ggcaggcgcg gcagcgcgcg ctgcacgcgg ttctgcacct gcgtttcggc ctgctcgacg
    15241 tcggtgccga cctggaaggt gacggtcagc gtcaaactgc cgtcggaggt ggcctgcgag
    15301 gacatgtaca gcatgttttc cacgccgttg atctgctctt ccagcggcgc ggcgacggtt
    15361 tcggcaatga ccttgggatt ggcgccggga tagattgcac gcaccaccac cgagggcggc
    15421 acgacttccg ggtattcgga taccggcagt ctgaatatcg ccagcacgcc ggcgatgaag
    15481 atgagtatcg acagcacgcc tgcgaagatc ggacggtcga tgaagaaacg ggaaatgttc
    15541 atgatgagat cttgtgcagt gtccgggcaa gccgggggca tccttccgcg cccgcggcgc
    15601 ggaaggtgct tttgtctgtg cgaagaatca ggactgcggt tgggacgact ggcccggcgc
    15661 actcgctgcc cgttcgcctt cggcctgcat cggcacgagc tgggcagtga ccggtgcgcc
    15721 ggggcgcacg cgctgcaagc cgttgacgat gatcttctcg ccgggcgtca ggccgctgcg
    15781 caccacgcgc aagccatcga cagcgggccc gagcgtcacc ggacggtatt cggccttgcc
    15841 ttcgccatcg acgacataaa catacttgcg gtcctggtcg gtgccgactg cacgctcgct
    15901 gatcagcgtc gcgcgtgtct cgccgccggt gccattgccg cttgcgagct ggatgcgggc
    15961 gaacaggccg ggcgcgagcg cgccctccgg gttgcgcagc accgcgcgca tgcgcacgct
    16021 gccggtggcg ggatcgatgc ggttgtcgac gaattccagt ctgccttcat gcgggaagcc
    16081 gctctccccg gccaggccga tgcgcacggc aacggccgtc cccttgcgcg ccatcgcacc
    16141 gacgcgcagc caggattcct catcgccgtc gaagctcgca tagatcgggt cgcccgagac
    16201 caccgtggtc agcaccacgt tcgcatcgac cagattgccg gcggtgacct cggccttgct
    16261 gatgcgaccg ccgatcggcg catgcacgcg ggtgaatccg agattgaggc gggcggcttc
    16321 gtgcgccgcc cgggccgccc gcgccgccgc ttccagctcc ttcgccgacg cagccttttc
    16381 atccgcctcg cgctgtgcga tcgcccgctc cgcaaccagg cgttcggcgc gagcgagttc
    16441 gaccttcgcc agttccgcac gggcacgcgc agcggcagcc gcagcctcgg tgcgcatcgc
    16501 ctcggcttcg tacggtcgcg gatcgatgac gaacaacagt tcccctttct tcaccaccgc
    16561 ccccggcgtg aagtgcaccg aagagatgaa gccggagacg cgcgggcgca cctcgacccg
    16621 ctcgaccgct tccagtcgtc cggagaattc ctgggtttcg acgatggttt tttccagcac
    16681 ttcggcggcc gataccgggg ggccggcctg cgctgcgtcg ccgagctcct gcgcgaccgg
    16741 tttgccgcag gcggcaagca gcagtgcggc caaggcactg gccgtgagaa cggctgcacg
    16801 cgccgcgggg gacaaggtaa ggagggtttt catgttgttc ttccttcgtt ttaagttctc
    16861 tgtgttcctg tagccacctt ccggtggcgg caagatggat tcaaatgacg tccaatcccg
    16921 cgaggaattg aacgatgtgt tccagcatct gtgcgctgcg tgcctcatcg cagcgcacac
    16981 cggccagcgt gggcgccgcg gccggcgcaa gccgtgcaag cgtggtacgc acgccggcgg
    17041 cggccagctt cgccccatac tgttcagcct cgtcgcgcag cgggtcatcg ctgaccgaca
    17101 ggatcagcgc aggcggcagc cccttcaggc ggctgacctg caacggacac gcatacggat
    17161 gcatgcgatc ggctgcatgc ggcaggtagc cgcggtaggc ggcgacgcag cgcgcaactt
    17221 ccgaggcggt gacgcgctcg gcctgccgct gccgcatcga ttcactgcac agccccggat
    17281 cgagcattgg catcagcagc atctgcccgg cgatgcgcgg cccgccgcga tcgcgcgcca
    17341 tcagcgccgc cacggcagcg agattgccgc ccgcctccat ccctgccgct atcaggcgcc
    17401 ggccatccca tcccagaccc gcgcgctccc gcatcgccca tgccagcacc gcgtaggcat
    17461 cctcggctgc agccggaaac ggctgcaccg gcgccgtggt gtaagtcgat gcgagcaccc
    17521 ggtagccgct cgcccgcgca aggccggtcg ccaggccgtc gaccgcgtcg agatccgccg
    17581 tttcgaatcc gccgcacgga aacagcacga ccaggcccgc tgcccgcgca cctgccggtg
    17641 catacagccg cgctgcgacg gcgctgcgcg cgcctgcagc cggcatctgg cgcacgagca
    17701 gttccgcgtc cacgccggca ccgcttcggc ccgttcgcct cgtcatccgt cccggcccag
    17761 ttcgcatcac cgttccctgc ctttctgtca tgcggcggac tatagctttg ttctagaatc
    17821 cgaataaaga cgcgccatgc gaattcaatg ttcaagaatc tgaacaatga cggcttgcat
    17881 caacgtgcag acactgcact gtaggcgggg gatgctgaca gcctgatgtc agcagatgtg
    17941 ggaaaggcaa cgaggagatt gcggtaatgg acaagcttca ggctatggaa gtgttcgtgc
    18001 aggtcgtcga cagcggcggc ttcacacgcg cggcggaaaa catgcagctg cccaaggcaa
    18061 ccgtttcgac gctggtgcgc gacctggaag catcactgtc ggtgaaactg ctgcaccgga
    18121 ccactcgacg cgtgagcgtg acggccgatg gcgcggccta ttacgaacgc tgcctgcgaa
    18181 tacttgcgga tgtgcgcgaa gccgaggacg cgctctctca cacccgcatg agtccgcgcg
    18241 gacggttgcg cgtggatgtg ccgaccgcgc tgggacggca tgtgctgatc ccggcgctgc
    18301 cggacttcct cgaacgctac cccgacatcc agcttgatct gggatgcagc gaccgcgcgg
    18361 tcgacctcgt cgaggaaggg gtggattgcg cggtacgcgc cggtccgctg gacgacctct
    18421 cgctgatcgc ccgccgtgtc ggcgagctgc atgtggtgac ctgcgccgcg ccctcctacc
    18481 tcgcactgca cggacggccg gcccatccat cggagctgtt gcggcatcgc tgcctcaatc
    18541 atttttcttc gcgcagcggc aaggtcatgg aatgggactt ccagcgcggc gacgaacgcc
    18601 tccagctgaa ggtaccgggg cggctggcgc tgaacgattc cgacgcctac atggcggcgc
    18661 tgctggccgg gatgggcatt gcgcgcacca tggtcacgtc aatctgcccg tacatcaagg
    18721 ccggtcagct ggaacttgtg ctgggcgact ggtgcgtcga gccggtgccg ctgcacgtgg
    18781 tctatccgca gaaccgtcac ctgtcggcga aggtgcgcgt attcgtcgaa tgggttgcgc
    18841 agacgctggc cgagcatccg tcgatgcaag tgccggaagg cccttgcccg ctgcatacgg
    18901 cctgcatttc agactcctat ggcgacggct tccagccgtc cgtgcccgcc tacagcttcc
    18961 ctaccagcca tgtggaaacc agcgtgacga cgacgagcgc gagatagaca aaggtggaca
    19021 tggcaggcgc aagcgaaaac ggaaccggaa agtgtgccgc gaccaggcac gtgtaatttg
    19081 accggcagca agaggtttgg ttgcggctat tcctctttca gcgtcctgag gcgcacgaga
    19141 taggccgtca cgcaaaccgc gatcaccagc agcatcgcct tgtgccacca ggtcggcacg
    19201 atggtcatcg agaacgccat cgagatccac atcagcgcga tcgccgtcac cttcgcgcgc
    19261 aacggcaccg cacgtttctc ctcaaaatcc cggatgaact tgccgcacca gcgattgttc
    19321 agcagccagc ggtgcagccg gtccgagccg cgcagaaagc aggcggaggc cagcagcagg
    19381 aaaggcgtgg tcggcacgag cggcagaaag atgccgatca cgcccagcgc gacggcgacc
    19441 aggccgatgc agttgaggac caccttgatc atgcggagcc ccgcggatgg tgctgcgcat
    19501 gcagccgctt gaggcgttcg cgtgcgacgt gggtgtagat ctgcgtggtg gaaatgtcgg
    19561 cgtgaccgag cagcagctgc acgacgcgca ggtcggcgcc atggttgagc agatgggtgg
    19621 caaacgcatg gcgcagcgtg tgcggtgaaa gcggtgcatc gatgccggct gccaacgcat
    19681 gcttcttgat cagcgtccag aacatctggc gcgtcatcgg gcagccgcgg ccggtgacga
    19741 acagcgcatc gtcgctgcgc ccgttgagga tgaccggccg cgcctccttc aggtagcgct
    19801 cgatccacgc gcgcgccact tcgccgaacg gcaccagccg ggtcttgctg ccctttccgg
    19861 tgatgcgcag cacgccttcg ttcatgccga cctcgatcga cttcagcagc accagctcgg
    19921 tcacgcgcag gccgctcgcg tacatcagtt ccagcatcgt gcggtcgcgc aggccgagcg
    19981 gagtcgatac gtccggagcc gcgagcaagg cctcgacctg cgcctcggac aaggtcttcg
    20041 gaaagcgtgg cggctgcttc gccgttctca gcttcaggca gggatcggtg gagatccggt
    20101 tctggcgcag cgcatgccgg aagaagcgct tgaagaccga caggcgccgg ttggccgaag
    20161 tcgccttggt ctcttcgtga cgggtggcga aataggcgct cagatcctcg ccatccgccg
    20221 catacagctg cttgccgcgt gcgcggtgca accagtcggc gaacagcgcc atgtcacgcc
    20281 ggtaggcctc cagcgtgttc ttggcaaggc cgtcctccag ccacaggctg tcgcagaatt
    20341 cgtcgatggc cgcctggctt gccgctattc cgttcatgcc caggcggtga tcccttcatg
    20401 ggcgagcagc cagcgcttga cgccgagatg aaagccggtt tcatcgtcat gattggagaa
    20461 tccgccgagc ccgccggtgg cggtcacccg gtggcaggga atcacgatcg gaaaccagtt
    20521 tgccccacag gcctgcccga tcgcacgcgg cgcggagcgg atcagtttcg cgatctggcc
    20581 gtaggtcgcc acctcgccgc gcggaatcgc actgatcgcc gcccacacgc ggcgctggaa
    20641 atcggtgcca acagaggcca gcgggagatc gaagcgaaaa tccggctcga tcagatagcg
    20701 ctcgatctgt cgcgcggccc gttcggccag cgcgtcggcc ggcgccgctt cggcgaagct
    20761 cggggggaga tacaccagct ccctgagcat cgcggcatcc gtgcggatgc cgacgccgcc
    20821 gaaaggcgcc ttcacgatcg cggaaaaaac actggctggg gcgggggaaa tcatcgcgct
    20881 agcataagcc aaatcggccg gccggcgcat ctgttgcgtc gcgctgccgc ttcgcaaaaa
    20941 caaaatggcc gcaccatcgg gtgcggccat tcgggatgag acgggatgtc gatcagtcag
    21001 cgtaggcgcc ggctgcgcgg atcaccggac cccacttgtc gatctcggcc ttcaggtggg
    21061 cgcgcagcgc gtccggagtg gcgcggttct tcgcgaccgg ctcggtgccc agttcagcga
    21121 aacgctgctt cacggtcccg tcttccagcg caacctgcag cgcattggtc aggcgatcga
    21181 ttgcgacctt cggcgtgccc ttcggcgcgt acacaccgtg ccacacggcc acttcgaagt
    21241 tcggcagacc ggcttcatga atggtcggga cgttcggcag cgacggcacg cgagtcttgg
    21301 tggtcacgcc gtagaccttc accttctccg cccggatctg gccggtggtg ttggtggtct
    21361 ggtcgcacat caggtcaacc tggccgccca gcaggtcgtt catcgccggg ccggtgccct
    21421 tgtacggaac ggtggtcatt tcggcgccga tcgcgctcat gaacagggtg ccgcacagat
    21481 gcgaggccga acccaggccg gcgttggcgt aggtcacctt gtcgcggttc gccttgatgt
    21541 gcgagatgac ttccttcagg tctttcggcg ggaaattgcc gcgcgcgatc agggtcatcg
    21601 gcacgtcggt caccaggccg atcggttcga agtcggtgat cgggttgtag gaaagcctgc
    21661 ggtacagggc cggagcggtc gacatgccga tgtggtgcag cagcacggta tgaccgtcgt
    21721 tgtcggcctt ggccacgcgc gcgacaccga tggtgccgcc ggcgccgccg acgttttcga
    21781 cgatcacctg ctgaccgagt gtcttgctca tggattgcgc gatcaggcgc gccaccgtat
    21841 cggtcgggcc gccggccgcg aacggcacca ccatggtcac cgtcttggtc gggtagttct
    21901 gggcatacgc ggatgtgctg gcaaccagcg caaggccggc gatgaggccg atggacttga
    21961 ttttcatgag cattgctcct ctgtattggt ttgatctgaa cggaaggcgc tcgcgcctgc
    22021 caagggccgg tctgaagccg ggcctgtccg ctgcgggcac aatgcctgac cgggttcggc
    22081 agggccgatt ctccagatta agcatgtctc cggcgcagat tattgcgacg gcgctcgcgc
    22141 atccacaatt gtggaaagcc gcaattattg cataaccaat tgtcaatcgt tgcgggaatt
    22201 gctgcacagc aagtcgcacg tcggcagaag aggccgcgcc gggcgcgccc ggccgaatca
    22261 gtactcggac gcgatctctc gcttgaccgc gttgatcagc gccttgccga tgcgcgcttc
    22321 catttccttg tgcaccggct ccagcgccgc gcgccaggtc tccttgtctt cctcgctcag
    22381 tacgtgaacg atggttctcc ccgacgtgcg gaccgcctcc agcgcggcat cgttttcctt
    22441 ctgcgcgatc gcattcgcat accgggttgc ctcgtccatc gccccctcga gcgcactgcg
    22501 gatgccggag ggcaggccgt cccagaattt cttgttcacg atcaccgcgt agccgaggta
    22561 gccgtggtcg gacacggtca ggtgacgctg cagctgatgc atgttctgcg tatagaagtt
    22621 ggacggcgga ttctcggtac cgtcgacgac gcccgcctgc agcgccttgt acacatcgcc
    22681 gaagccgagc gagatcggat tggcgcccag cgcgcgcatc tgcgcatcga gcacgcgcga
    22741 aggctggacg cgcatcttca ggccgcgaaa atcctcgggt tcgtgtagcg ggcgattcgc
    22801 ggacattacc ttgaagccgt tgtcccagta agccagcccc ttgatgccct tggcttccag
    22861 cttgtgcagc agcacctgcc cgatcgggcc ttccttgacc cgatgcaggg cctccttgtt
    22921 gggaaagatg aaaggcagat cgaacacctc gaactcgcgg atgccgagcg gaccgaactt
    22981 ggccagcgac ggcgcgagca tctgtaccgc cccgagctgc agcgcctcca gctcctcgcg
    23041 gtccttgtac aactggctgt tcggatagac gtcgacgcgc acgcgcccgt tggtggcttt
    23101 ttcggcaagc tccttgaagc gcagcgcggc cttgcccttc ggtgtttccg gcgcgaccac
    23161 gtggctgaac ttgatgacga tctgctcctg cgccgcgacg agcgacgaga agcaggcggc
    23221 gaaggcggcg gcaatgatga gaatgcagga tttgaacgtc atgtctgtcc ggcagggcgg
    23281 ggaataagcg cgtcgccggc ctggcattgg catcgagcga ttgtcgggat gccggcgcaa
    23341 gggctcaagt gtggataacc acaacgcgcg gccgacaaga atacacgcgg gcgctcggct
    23401 acactatctg cactcatgcc ggccctcctg aatttcgccc cgctcgcccg ccgccgccgc
    23461 atctggcaat ggctggtgcc gatgctgctc gtcctgttgt ttctgatcac cctgctctgg
    23521 ctgccgcgcc aggcccagca gatggaggcc aacgaccgtc aggaacagct gatcgcagac
    23581 accttgtggg tggagcagac catccgtttc cagcttgaac gcatcgagga cggcatccgg
    23641 ctgatcgcca gcgagatcgg caccggctac ctcggcggcc agcgcctgcg tgagcgcatg
    23701 accatcctcg accgcaacaa ccgcgagctt gcgcgcctgg tctggttcga tgcgaacggc
    23761 aacagcgtcg cctcgaccga cgaccgcgca ctgcggctgg aggacttgcc ggccgcatcg
    23821 aggctgaccg ccgagcatgc gcgggagacg cgcaccccgc agtacagcca gccggcgccg
    23881 ccgaacgggt tgaccgggcc gatcctgatg gactaccact tcccgatctt ccagaacaac
    23941 cgctatctgg gcagcctggt ggccacgtac cacatgtcgg caatcctgga cgagatggtg
    24001 ccgtggtggt tcgcgcagga caacgagatc gtgctgaccg atgccgacga caacgtgctc
    24061 gcgcgccgcg cagccggcgg cgccggccgg ggcgtctaca cgcattcgcg cgacctgcac
    24121 ctgccgtatg cgaacgtgac cctgcgcacc aacagcgtca agagcgcgcc caagctcctg
    24181 cccaacctgc tggtcggctc ggtgatcgcg ctgtcgatcg gcctgatctg gagcctgtcg
    24241 gcgctgtgga aggacatcaa ccggcgcatc gctgccgaag gcgcgttgcg cgagcaggtg
    24301 gcattccgcg cggcgatgga aaactcgctg gtcaccggcc tgcgcgcgcg cgacctggag
    24361 gggcgcatca cctacgtcaa tccggcgttc tgcaacatgg tcggcatgcg cgccgaggac
    24421 ctgctcgggc acaagccgcc gatgccctac tgggcgccgg aggcgatgaa cgaataccag
    24481 gagcgctatg cgcagatgct ggccggcacg gtcaagccgc aggggcacga aacgatcttc
    24541 cagcgcgccg acggcgagcg cattccggtg ctggtgttcg agtctccgct ggtcgacgac
    24601 agcggccgcc agaccggatg gatgggctcg atcctcgata tctccgatcg caagcgcttc
    24661 gaagacctga accggcgcca gcaggaaaag ctgcaggcca gcgcgcggct ggcgacgatg
    24721 ggcgaaatcg cctccacgct cgcgcacgaa ctgaaccagc cgctggccgc catttccagc
    24781 tacacgaccg gcgcgctgaa cctgctcgac agcgaggccg cccgcgccgg gacgctggat
    24841 accgcgatgc tcaagggcgc gctcgaaaag gccaacgcgc aggcgcagcg cgccgggcac
    24901 atcatccgca gcgtgcatac cttcgtgaag cggcgcgaac ccagccgcga gaaggttgac
    24961 ctttcggcat tgatcgacag cgtgatcccg ctggtggaac tgcaggcgcg ccagttcttc
    25021 gtggtgatcc agaccgaggt ggaagacaag ctgccgccgg tgcttgccga cccggtgatg
    25081 ctcgaacagg tgctgctgaa cctgacgcgc aacggcatcg aagcgatgca ggcggttcca
    25141 cctccgcgcc gacgcctgcg catcaccgcc ggcttggacc gccagtctcc ggtcagcgtg
    25201 gtggtctccg tgaccgacca gggccacggg attccggacg aggtcgccga gcgcctgttc
    25261 tcgccgtttt tctccaccaa ggccgaaggc atgggcatgg gcctgaacat ctgccgcacc
    25321 acgatcgagt tccatggcgg gaagctcagc tatgcggcga accccgccgg cgggaccatt
    25381 ttccgcttct ccctgccggt ttcagcgacc gcaggcatcg aaacagtagc cgccgccccc
    25441 gtaatcagtg gaaaataacg cgccgggctc ctgcactgcc cggcaatacc acttaacaag
    25501 aatgccgaat cgagccggca ctcacgggga cccactggat gctgcatata gtcgacgacg
    25561 aggaagtcat tcgcgattcg ctgagctggc tggcacagtc gcgttcgatt ccgactgccg
    25621 tctatgacag cggcgcggcc ttcctcgccg cgctcgggcc cgacaaggcc ttcgaccagg
    25681 agggcgaatg cgtgctgctc gacgtgcgca tgcccgacat gagcggcgcc gtcctgttcg
    25741 acaccctcgc caaccgcggc ctgatccagc ggctgcccgt catattcctg accggccacg
    25801 gcgacgtacc gatggcggtg gacagcctga agcgcggcgc cttcgatttc ttcgaaaaac
    25861 cgttcaacga caacaagctg atggaccgcg tgcgcgaggc gctggccgcc tcgcgcaagg
    25921 cgctcgacat ggcagaagtg cacgcgcggc tggccaatct gtccgcacgc gagcgcgaag
    25981 tgctcgatct gattctggcc gggaagatga acaaggtgat cgccgatgaa ctcgggatca
    26041 gcatgcgcac cgtcgaggtg catcgcgcgc acatctttga caagatgagc gtgaagaccg
    26101 cggttgagct ggcccgcctg ctgaaatagc gccgccccgc gccagcgtcc agcgtgaaag
    26161 accggtaaca ttgccggtcg ccaagctgtc gctttacctc ctccatgtcc atcgccacca
    26221 tcctgttccc cgacttcgcg ctgatcctgc tcggcttcct gctgatgcgc tatgcggact
    26281 gggggaacgg cttctggagc gggctggaaa agctgatcta ctacttcctg ttcccggccc
    26341 tgctgttcaa caccacctcg cgtaccgagt ttgatttcgc cagcaccgga gtgatgctgc
    26401 aggtcgccgt gatcggcatc ctcagcgcaa tcgcactcgg ctggctggca aagccgctgt
    26461 tccgcgcgcc gccgatgatg ttcgagtccg gcgtgcagac cgcctttcgt ttcaattcct
    26521 acattgcgct ggccgtcgcc tcgcgcctgg caggcgacaa gggcacgacg ctgatggcgc
    26581 tgatcatcgg cttcgcggta cccctttgca atatggcagc cgtgcatgct ctggcgcgca
    26641 agaacggtcg cctgctgcgc gaactggcgc gcaacccgct tctgctcgcg accgccagcg
    26701 gcatggcctt caatctgctc ggcctggagc tgccggagat ggtcagcgcc acgctggggc
    26761 gcatgggcaa cgcatcgatc gcgcttggcc tgatcgccgt cggcgccggg ctgcgcctgt
    26821 cgggggtgga ggccggaaag gacatggccg cctacttcat cgccgtcaag ctgctggcca
    26881 agccggccat cgcgctgctg ctcggcatgg cgctgcaact gccgccacca caattgcaga
    26941 tcgtcgtggc cttctgcgca atgccgacgg cgtcgagcgc ctacgtac
//
LOCUS       DATLGF010000039         4443 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_96771,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000039 DATLGF010000000
VERSION     DATLGF010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96771"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..630)
                     /locus_tag="VM406_01240"
     CDS             complement(7..630)
                     /locus_tag="VM406_01240"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HVL74612.1"
                     /translation="MSQFFHIHPENPQLRLVRQAAQIIHAGGLVALPTDSSYALVCHL
                     DDKAAVERLRRIRGVDEKHHLTLLCRDLSEIAQYAKVDNRQYRMLKAATPGPYTFILE
                     ATKEVPRRLSHPSRKTIGLRVPENRIAHALLEELQQPLLGATLILPGDEEALNDPEEV
                     RERLEKQIELVIDGGACSMQPTTVIDLTGAEPELLRAGRGDPSLFGL"
     gene            complement(680..1552)
                     /gene="htpX"
                     /locus_tag="VM406_01245"
     CDS             complement(680..1552)
                     /gene="htpX"
                     /locus_tag="VM406_01245"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HVL74613.1"
                     /translation="MKRIVLFLATNLAVLVVLSVVMSLTGLHRFVTAEGLDLGALAVF
                     SLVVGFTGAFISLLLSKPMAKWTTKARVIESPSSSTELWLLETVKKLSQKAGIAMPEV
                     AIYEGEPNAFATGAFKNSALVAVSTGLLQSMTKEEVEAVLAHEVAHVANGDMVTLTLI
                     QGVVNTFVVFLARVVGYFIDKAVFRSERPGIGYFGTVIVCEIIFGILASMIVAWFSRY
                     REFRADAGAAGLMGTPQPMVKALARLGGLHAPDSMPQAYKAMGINSGRSGFSALFATH
                     PPIEERIAALRNAR"
     gene            complement(1663..2499)
                     /locus_tag="VM406_01250"
     CDS             complement(1663..2499)
                     /locus_tag="VM406_01250"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HVL74614.1"
                     /translation="MHNVDLHCHSTVSDGALTPSAVAERAHAHGVTFWALTDHDELGG
                     IPEARAAAQALGMGYATGVEISVTWAGRTVHIVGLGFDENDETLRAGLAETRSGRERR
                     ALEMALQLRSVGIEGAFEGALKYVGNPDLISRTHFARYLVEIGVCENTNEVFRNYLVE
                     GRPGYVPHRWARLADAVSWIRGAGGVAVVAHPGRYDFTDLELEAFLDEFKRAGGTGIE
                     VITGSHTPDEYGRFAAVARRHGLLASRGSDFHAPGESCTEFGSLPQLPADLKPIWHDW
                     RM"
     gene            complement(2605..3480)
                     /locus_tag="VM406_01255"
     CDS             complement(2605..3480)
                     /locus_tag="VM406_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL74615.1"
                     /translation="MTPSRSEFVQRRGLRLHLRHWGREDAPRLFMLHGWMDVSASFQF
                     VVDCLRQDWHVIAPDWRGFGLSERTRSDCYWFPDYIGDLDAILEHCAPNESVNLLGHS
                     MGANVAMLYAGIRPERIAKLINLEGFGMAASDPAQAPKRYAEWLDQLRTGAQMRAYGS
                     MEEVAARLRKTNPRLSVARAEFLARHWAAPNAAGEWEILGDPAHKLPNPVLYRVEEVL
                     ACWRAISAPVLWVEADDTEAWRWLGGKEKGRAEVDRRIACISEVRTATIAGAGHMLHH
                     DQPECLAALIEDFLS"
     gene            3609..>4443
                     /locus_tag="VM406_01260"
     CDS             3609..>4443
                     /locus_tag="VM406_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HVL74616.1"
                     /translation="MKAAKAPQVPSQGDRYQELYESFRWHVPADLNIAQVCCLRWADD
                     KRRIAVYYEDEAGQTATLTYAALHEQAARLANVLRGLGVSKGDRVAIVLPQRTETAIA
                     HIACYLLGAVAMPLSILFGPEALEYRLQNSEAGVAIVDQAGYQNLQQARASCPNLRHV
                     LTVSCDGGDDLDWHAELSKAGNRFEPVATTAQDPAVLIYTSGTTGAPKGALIPHSAII
                     GNLTGFVASQNWFPQPGDVFWSPADWAWTGGLWDALLPTLYFGMPIVGYRGRFSPESA
                     FS"
BASE COUNT          807 a         1462 c         1431 g          743 t
ORIGIN      
        1 tggcgcttac aggccgaaca gggacgggtc gccgcgtccg gcgcgcagca gttccggctc
       61 ggcgccggtc aggtcgatga ccgtggtcgg ctgcatgctg caggcgccgc cgtcgatcac
      121 cagctcgatc tgcttttcca gccgttcgcg cacttcctcg ggatcgttga gcgcctcctc
      181 gtcgccgggc aggatcagcg tcgcgcctag cagcggctgt tgcaattctt ccagcaacgc
      241 atgcgcaata cggttttccg gtacccgcag gccaatggtc ttgcgcgaag gatgggagag
      301 ccggcgcggc acttccttcg tcgcttccag gataaaggtg tacggacccg gcgtggcggc
      361 cttgagcatc cggtactggc gattgtccac cttcgcatac tgggcgattt cggacaggtc
      421 gcggcacaac agcgtcagat ggtgcttttc gtcgaccccg cggatgcggc gcaggcgctc
      481 gaccgccgcc ttgtcgtcca ggtggcagac cagcgcgtag ctggaatcgg tcggcaacgc
      541 aaccagtccg ccggcatgga tgatctgggc ggcctgcctg accaggcgca gttgcgggtt
      601 ttccggatgg atatgaaaaa attggctcat aaaaaaatag cggcggtcgc tcggaccgcc
      661 gcttgtcgat tcgattcgat cagcgtgcgt tgcgcagggc tgcgatgcgc tcctcgatcg
      721 gcgggtgggt ggcaaacagc gccgagaacc cggagcggcc gctgttgatg cccatcgcct
      781 tgtaggcctg cggcatgctg tccggcgcgt gcaggccacc caggcgagcc agcgccttga
      841 ccataggctg cggcgtgccc atcaggcccg ctgcgccggc atcggcgcga aactcgcggt
      901 agcgcgagaa ccaggcgacg atcatggaag ccagaatgcc gaagatgatc tcgcagacga
      961 tcacggtgcc gaaataaccg atgccaggac gttcgctgcg gaacaccgcc ttgtcgatga
     1021 agtagccgac cacgcgcgcg aggaacacga cgaaggtgtt gaccacgccc tggatcaggg
     1081 tcagcgtcac catgtcgccg ttggccacgt gcgcgacttc atgcgcgagg acggcctcaa
     1141 cctcttcctt ggtcatgctc tgcagcaggc cggtggaaac tgcgaccagc gcggagttct
     1201 tgaaggcgcc cgtcgcaaac gcattcggct cgccctcgta gatggcgact tcgggcatcg
     1261 cgatgcctgc cttctgcgac agcttcttga cagtctcgag cagccaaagc tcggtcgacg
     1321 aagacggcga ttcaatcacg cgcgccttgg tcgtccactt cgccatcggt ttcgagagca
     1381 ggagcgaaat gaacgcgccg gtgaagccga ccaccagcga gaacacggcg agtgcgccga
     1441 gatccaggcc ctcggccgtc acgaaacggt gcaggccggt cagcgacatc acgaccgaaa
     1501 gcacgaccag cacggcaagg ttggtcgcaa ggaaaaggac aatacgtttc attcacagcc
     1561 ctcctgtcaa gcgtagggga aagatggggt tctcgaaggg aatttcaagc ggcgccatga
     1621 tgccacaggc catgcccgtc cgacagcagg caaaagcgaa catcacattc gccagtcgtg
     1681 ccagatcggc ttcagatcgg caggcagctg cggcagcgag ccgaactcgg tgcacgactc
     1741 cccgggggcg tgaaaatccg agccgcgcga ggccagcagg ccatgccgcc gcgcgacggc
     1801 ggcgaagcgt ccgtattcgt ccggcgtatg gctgccggtg atcacttcga tgccggtgcc
     1861 gccggcgcgc ttgaattcat cgaggaaggc ttcgagctcg agatccgtga aatcgtagcg
     1921 tccgggatgc gcgaccacgg cgacgccgcc ggcgccccgg atccaggaga cggcatcggc
     1981 caggcgagcc cagcggtgcg gaacataacc gggcctgccc tccacaaggt agttgcggaa
     2041 cacctcgttg gtgttctcgc acacgccgat ctcgaccagg tagcgggcaa agtgggtgcg
     2101 cgagatcagg tcggggttgc cgacgtactt gagcgcgccc tcgaaggccc cctcgatacc
     2161 caccgagcgc aactgcaacg ccatctcgag cgcgcgccgc tcgcgcccgg aacgcgtttc
     2221 cgccagaccg gcgcgcaggg tttcatcatt ctcgtcaaag ccgagaccga cgatatgaac
     2281 ggtgcgtccc gcccaggtca ccgagatttc cacgcccgtc gcgtaaccca tgcccagcgc
     2341 ctgcgcggcg gcgcgggctt ccgggatgcc gccgagttcg tcgtggtcgg tcagggccca
     2401 gaaagtcacg ccgtgcgcgt gggcgcgctc ggcgaccgcc gaaggcgtca gcgccccgtc
     2461 ggaaacggtg gagtggcagt gcaagtcgac gttatgcatg gtggatgttg atagcggcat
     2521 gatgatcatt ttacgcgctg ggcccctgcc tcgcgcagcc ccgccaccgc ttggaccgcg
     2581 gatccgccat tgcgatccgc caattcagga gagaaagtcc tcgatcagcg ccgccaggca
     2641 ttccggctgg tcgtgatgca gcatgtggcc ggcgccggcg atcgtcgcgg tgcgcacctc
     2701 gctgatgcag gcgatgcggc gatcgacttc cgcccgccct ttctccttgc ctcccagcca
     2761 gcgccaggct tcggtatcgt cggcctccac ccacagcacc ggtgcgctga tcgcgcgcca
     2821 gcaagccagc acttcctcga cgcggtacag caccggattg ggcagcttgt gcgcgggatc
     2881 gccgaggatt tcccattctc ccgccgcgtt cggggccgcc caatgccggg ccaggaattc
     2941 agcgcgcgcc acggacagcc gcggattggt cttgcgcagc cgtgcggcaa cctcctccat
     3001 gctgccgtag gcgcgcatct gcgcaccggt gcgcagctga tcgagccact ccgcgtagcg
     3061 cttgggcgcc tgcgccggat cgctcgccgc catgccgaaa ccctcgaggt tgatcagctt
     3121 cgcaatgcgc tcgggacgga tgccggcata cagcatcgcg acgttcgcgc ccatgctgtg
     3181 tccgagcagg ttgacggact cgttcggcgc gcagtgctca agtatcgcgt cgagatcgcc
     3241 gatatagtcc gggaaccagt agcagtccga ccgcgtgcgc tcggacaagc cgaatccgcg
     3301 ccagtccggc gcgatcacat gccagtcctg cctgaggcag tcaaccacga actggaatga
     3361 agccgacacg tccatccagc cgtgcagcat gaaaagcctg ggcgcgtctt cgcgacccca
     3421 gtggcgcagg tgcaggcgca gtccgcgccg ctgaacgaac tcggaacgcg acggggtcat
     3481 tgacacggct cccggcgcgc tttggatggc ggataaatcg gcataatagg catctccatc
     3541 ggcttgtgag acgacatccg tggacgtcaa taaaagaacg atcgttcgat aagtataaag
     3601 ggagtgagat gaaagctgcc aaggccccgc aagtcccgtc gcaaggcgac cgttaccagg
     3661 aactgtatga atccttccgc tggcatgttc cggcggacct gaacatcgcg caggtctgct
     3721 gcctgcgctg ggccgacgac aagcgccgca tcgcggtgta ctacgaggac gaagccggcc
     3781 agaccgccac cctcacgtac gccgcgctgc atgaacaggc cgcgcgactg gcgaacgtgc
     3841 tgcgcggcct cggagtgagc aagggcgatc gcgtcgccat cgtactgccc cagcgcaccg
     3901 aaaccgctat tgcccacatc gcctgctacc tgctcggcgc ggtggcgatg ccgttgtcca
     3961 tcctgttcgg ccccgaagcg ctcgaatacc ggctgcagaa cagcgaggca ggcgtggcga
     4021 tcgtcgacca ggccggctac cagaacctgc agcaggcgcg cgcaagctgc ccgaacctca
     4081 ggcatgtgct taccgtgtcc tgcgacggcg gcgacgacct ggactggcat gcagaactgt
     4141 cgaaggcggg caatcgcttc gaaccggtgg ccaccacggc gcaggatccc gcggtgctga
     4201 tctacacgtc cggcaccacc ggcgcgccga agggcgcgct gattccgcat tccgcgatca
     4261 tcggtaatct caccggcttc gtcgcttcgc agaactggtt tccgcaaccc ggcgacgtgt
     4321 tctggtcgcc ggccgactgg gcctggacag gaggcttgtg ggacgcgctg ttgccgacgc
     4381 tttacttcgg catgccgatc gttggctatc gcggccggtt ctcgcccgag agcgctttct
     4441 ccc
//
LOCUS       DATLGF010000040         7991 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_96887,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000040 DATLGF010000000
VERSION     DATLGF010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 7991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7991
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96887"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="VM406_01265"
     CDS             <1..1479
                     /locus_tag="VM406_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HVL74617.1"
                     /translation="GMNAPEVTVEVHLANGLPAFTIVGLPETEVKEARDRVRAALQNA
                     RFEFPARRITVNLAPADLPKESGRFDLPIALGILAASGQMPAEELHRYEFAGELSLSG
                     ELRPIRGALAMTYAMQRAGGPGRGFILPRANAEEAALVRDAAIYPADNLLQVCAHFAA
                     SDADSRLTRHQYGTEQVVPSYPDFAEVKGQGQAKRALEVAAAGTHSVLMMGPPGSGKS
                     MLASRFPGILPAMTDDEALESAAVQSLTGGFSAAKWKMRPFRSPHHTASGVALVGGGG
                     TPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLESGHITISRAARQADFPARFQLI
                     AAMNPCPCGYLGHHANKCRCTPDQVLRYQGRISGPLLDRIDMQIQVAALPHDDLLRQG
                     DGETSATIGERTAAAFRRQLERQGKPNSALGPKDIDALCKPDNAAEQLLRTAMTRLNW
                     SARAYHRVLKVARTIADLADAKTISQPHVAEAIQYRRLLSEG"
     gene            1535..2425
                     /locus_tag="VM406_01270"
     CDS             1535..2425
                     /locus_tag="VM406_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL74618.1"
                     /translation="MLKTVIAGDLEIAYQERGSADAPAVILLHGFPYDIHGYDEVATQ
                     LAPSARVIVPYLRGFGPTRFLSAATMRSGQQAAIAHDLLALMDALSIPSAVLAGYDWG
                     ARAACIVAALWPQRVDGLVTGGGYQIQDIAAAAHPLVPDLEHRLWYQYYFHGERGRAG
                     LAANRFELGRLLWQQWSPSWAFSDETYAQSAAAFDNPDFVDVVVHSYRHRFGLVPGDP
                     RFDATEALLAQQPPILVPAVVLEGADDGVAPPLEQDRKRDLFGARYERRLIPGVGHNI
                     PQEAPQAFAEAIASLLPAAV"
     gene            2433..3281
                     /locus_tag="VM406_01275"
     CDS             2433..3281
                     /locus_tag="VM406_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVL74619.1"
                     /translation="MFNHPSEFVTFKASAHLPGVELYSARLIDHAFAPHVHDGYSIGA
                     IEAGVERFRYLGTEHVAPAGTLVLLNPDELHTGQAEVEAGWTYQMLYIEPDRLRAMNG
                     DEAWFPRAAVHDPQLSARFRHLFARLWNASDDLSFVSDFTVLVDAIVARYGRNAGPPV
                     HADARQLKRIAAMRRVLDCIEANLDQPLPLETLAAEAGMSLFHFVRVFTAAYHATPHQ
                     YVQARRAARAKALLARRAMPAEAAAAAGLTDQSHLNRWFKRAYGVTPAQYQQQIGTRP
                     PARPRA"
     gene            3327..4292
                     /locus_tag="VM406_01280"
     CDS             3327..4292
                     /locus_tag="VM406_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVL74620.1"
                     /translation="MLTGLLCALGAGLMWGLVFVAPLMLWDYPGLMLSFGRYLGFGLI
                     ALVPAFFDWTRIRALTRADWIAALKLSLVGNILYYAFLATAIQLADAPLPAMIIGTLP
                     LVISICSNWSPGHAGETIPWMRLAPSLLIIAAGLLLVNAGELAHLDGARSTADYLLGC
                     TVALGALAAWTWYPIRNARYLKAHPRIRSSTWATAQGLATLPLAAIGFAGYGIHTWLN
                     GGGYAFPAGPRPFDFIALMLTLGLCASWLGTLLWNKASQKLPTSLAGQLIVFETLAAL
                     LYAFLWRGAMPAPGVLAGIALLCAGVVLGMRAFRNTAGAATEAAT"
     gene            4385..4918
                     /locus_tag="VM406_01285"
     CDS             4385..4918
                     /locus_tag="VM406_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74621.1"
                     /translation="MRHSTLRRAAAAACCVLLLACSPKFDWREVHGSAAPYSALLPAK
                     PATHARDINLDGLPVTMTMTGAEVDSVTYAIGTIELAEPAQAAHALNAMRTALVRNIH
                     GTVTQEKSSAPADGTTMLELEANGPLPQGRRLLQARLIARERHVYQLLVVGDERDFSR
                     EAADTFFTSFRLSSTNN"
     gene            4923..6170
                     /locus_tag="VM406_01290"
     CDS             4923..6170
                     /locus_tag="VM406_01290"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL74622.1"
                     /translation="MSSSLKGVLAIRVFLSFAFAYFLSYAFRSVNAVIGPELRVDLSL
                     SNADLGLLSAAYFLTFAAMQLPLGVWLDKYGPRRTESALLLVAAAGSAIFAMSETAAG
                     LWTGRALIGVGVSGCLMAPFKAYRLWFPAERQAQLASWMLVAGTSGALASTVPVSAAM
                     PIIGWRGVFWVMCAMILAASAAIFFLLKRIEREHPAPVAAPSSTPVAGATYASIFGNP
                     FFRRMAILGAINLASFSALQTLWAGPWMVTVLGMEAHQSANVLFMFNLVLLCAYLVLG
                     WWAPRHVSYGGGAGIPALRVVAIGIGLAILVQFAIIAFTGTWSWMLWILLALCLTMNT
                     LAATAVSLHFPPASAGRANSSFNLTQFAGTFIVQWGIGLLIDAFGTAGASSVDAMRLA
                     FAVCAGVQALALAAFLLAGRRSA"
     gene            6241..6576
                     /locus_tag="VM406_01295"
     CDS             6241..6576
                     /locus_tag="VM406_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HVL74623.1"
                     /translation="MLITFKSKAAADILMYEKHAKPILDLLNRDVERGIITAAEMNDA
                     VARLEAAIEESRRREAQERAQREADSEEDEEDEAQPQQTVSFATRAHPLLEMMRAAAR
                     SGANIMWGV"
     gene            6604..7239
                     /locus_tag="VM406_01300"
     CDS             6604..7239
                     /locus_tag="VM406_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HVL74624.1"
                     /translation="MDTLGIVNFGLFVAAVLLLNVTPGPDTAYIVGRSLAQGRMAGLV
                     SALGISAGCCVHALALAIGLSALLQASAGAFTAIKLAGGAYLAWLGLRMLLSRPQNAA
                     AAPVAPARAMSMIFMQALATNVLNPKVILFFLAFIPQFVRPDAPHKALGFLVLGVALA
                     LISLAWNGATALLAGTLARGAGRNPTVRVWLERVVGASFIALGARLALGRS"
     gene            7241..>7991
                     /locus_tag="VM406_01305"
     CDS             7241..>7991
                     /locus_tag="VM406_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HVL74625.1"
                     /translation="MAGPARPRTPALRTDSLAYALSGAAHAVAIVAEGSALPQALASV
                     FRRLQAPPQARGAIQDIAYRTMRQRASSDALLAQLSDRPVSPPTLHALLACSLALLQP
                     AAGEDPGSLPYEAFTVVDQAVAAAGANPALARAKGMVNAVLRRFLREQAALLPALQHD
                     PVTRWNHPRWWIDALRSAYPDSWQAILEAGNRPPPLTLRVNLRRVTPEDYLARLAAAG
                     ISARQIGPQAVRLDRALPVGQIPGFDEGLVSV"
BASE COUNT         1210 a         2833 c         2588 g         1360 t
ORIGIN      
        1 ggcatgaacg cccccgaagt caccgtggaa gtccacctcg ccaacggcct gccggctttc
       61 accatcgtcg gcctgccgga aaccgaggtg aaggaagcgc gcgaccgggt gcgcgccgcg
      121 ttgcagaacg cgcgtttcga attccccgcc cgccgcatca ccgtgaatct cgcgccagcc
      181 gacctgccca aggaatccgg ccgcttcgat ctgccgatcg cgcttggcat cctcgccgcc
      241 tccggccaga tgccggccga ggagctgcat cgctacgaat tcgccggaga gctgtcgctg
      301 tccggggaac tgcggccgat tcgcggcgca ctggcgatga cgtatgcgat gcagcgggcc
      361 ggcggccccg ggcgcggctt catcctgccg cgcgcgaatg ccgaggaggc ggccctggtg
      421 cgcgacgcgg cgatctaccc ggctgacaac ctgctgcagg tctgcgcaca ctttgcagcg
      481 agcgatgcgg acagccggct cacgcgccac cagtacggca ccgaacaggt cgtgccaagc
      541 tatccggact ttgccgaggt caagggacag gggcaggcca agcgcgcgct cgaggtggcc
      601 gcggccggaa cgcacagcgt gctgatgatg ggcccgcccg gcagcggcaa atcgatgctg
      661 gcctcgcgct ttccgggcat cctgccggcg atgacggatg acgaggcgct ggaatccgcc
      721 gcagtgcaat cgctgaccgg cggcttctcg gcggcgaaat ggaagatgcg gccgttccgc
      781 tcgccgcacc ataccgcctc gggcgtggcc ctggtcggtg gcggcggcac accgcgcccc
      841 ggcgagatct cgctggcgca ccacggcgtg ctgtttctcg acgaactgcc cgagttcgac
      901 cgcaaggtgc tggaagtgct gcgcgaaccg ctcgaatccg gccacatcac gatctctcgc
      961 gccgcgcgcc aggcggactt ccccgcgcgc ttccagctga tcgccgcgat gaacccctgc
     1021 ccctgcggct atctcggcca ccacgcaaac aaatgccgct gcacgccgga tcaggtgctg
     1081 cgctatcagg gcaggatttc cgggcccctg ctcgaccgca tcgacatgca gatccaggtc
     1141 gccgcgctgc cgcacgacga tctgctcaga cagggcgacg gcgagaccag cgcgacgatc
     1201 ggcgagcgta ccgccgccgc gttccgccgc cagctcgaac gccagggcaa acccaacagt
     1261 gcgctcggcc cgaaggacat cgatgcgctg tgcaagccag acaacgccgc cgaacaattg
     1321 ctgcgcacgg cgatgacaag gctgaactgg tcggcgcgcg cctaccatcg cgtgctgaag
     1381 gtcgcgcgca cgattgccga cctggccgat gcgaaaacca tcagccagcc gcacgtggcg
     1441 gaggcgatcc agtatcggcg gctgttgagc gaggggtagg cgacacgcgc ggcggcggac
     1501 gattacactg acgcttcacc gatccgcttg tgccatgttg aaaaccgtca tcgccggcga
     1561 cctcgaaatc gcctatcagg aacgcgggtc cgccgacgcg ccggccgtga tcctgctgca
     1621 cggctttccg tacgacatac acggctatga cgaggtggcc acgcagcttg cgccctcggc
     1681 ccgcgtaatc gttccctatc tgcgcggctt cggtccgacc cgtttcctgt cggccgcgac
     1741 catgcgctcc ggccagcagg cggcgatcgc gcacgacctg ctcgcgctga tggacgccct
     1801 gtcgattccg tctgcggttc tggccggcta cgactggggc gcgcgcgctg cctgcatcgt
     1861 cgcggcgctt tggccgcaac gcgtcgacgg cttggtcacc ggtggcggct atcagatcca
     1921 ggacatcgcc gcggcggcgc atccgctggt gccggatctc gaacatcgcc tctggtacca
     1981 gtattacttt cacggcgagc gcgggcgcgc cggcctcgcg gccaatcgct tcgagctggg
     2041 tcgcctgctg tggcagcaat ggtcgccgag ctgggcgttc agcgatgaaa cctatgcgca
     2101 aagcgcggcc gcgttcgaca acccggactt cgtcgatgtg gtcgtgcact cgtaccgtca
     2161 tcgcttcggc ctggtgccgg gcgatccgcg ctttgacgca accgaagcgc tgctcgcaca
     2221 gcagccgccg attctcgtgc cggccgtcgt gctggaaggc gccgacgacg gcgtcgcacc
     2281 gcctttggag caggaccgca aacgcgacct gttcggtgcg cgctacgaac gtcgattgat
     2341 ccccggcgtc ggccacaaca ttccgcagga agcgccgcag gcctttgcag aagcaatcgc
     2401 ctcgctgctg cccgcggccg tctgagagca gcatgttcaa ccacccgtcc gagttcgtca
     2461 ccttcaaggc cagcgcgcac ctgcccggcg tcgagctgta ttccgcacgc ctgatcgacc
     2521 atgcgttcgc gccgcacgtg catgacggct attcaatcgg cgcaatcgaa gccggcgtcg
     2581 agcgtttccg ctatctcggc accgaacacg tcgcgccggc cggcacgctg gtgctgctca
     2641 acccggacga gctgcacacc ggccaggccg aggtcgaggc cggctggacc tatcagatgc
     2701 tgtacatcga gccggaccgg ctgcgcgcga tgaacgggga cgaagcctgg ttcccgcggg
     2761 cggcagtgca tgatccgcaa ttgagcgcgc gctttcgcca cctgttcgcg cgcctgtgga
     2821 acgcgtcgga cgacctgtcc ttcgtgtcgg acttcacggt actggtcgac gcgatcgtcg
     2881 cgcgctacgg gcgcaatgcc ggaccgcccg tgcatgccga tgcacgccag ctcaagcgca
     2941 tcgcggccat gcgccgcgtg ctcgattgca tcgaagccaa tctcgaccaa ccgctgccgc
     3001 tggagacgct ggcggcggaa gccggcatgt cgttgtttca tttcgtgcgc gtattcacgg
     3061 ccgcctacca tgccaccccg caccagtacg tgcaggcgcg ccgcgcggcg cgcgcaaagg
     3121 cgctgctcgc gcgacgcgcg atgcccgcgg aggccgccgc agcggccggc ctgaccgacc
     3181 agagccacct gaaccgctgg ttcaagcgtg cctacggcgt cacgcctgcg caataccaac
     3241 agcaaatcgg tacaagaccg ccggcccggc cgcgcgcatg atgcgggcac cggcgcagat
     3301 gcgccaattc cttttcttcc gcgatgatgt tgactggttt gctatgcgcg ctcggcgccg
     3361 gcctgatgtg gggacttgtg ttcgtcgctc cgctgatgct gtgggactat ccgggcctga
     3421 tgctgtcatt cggccgctat ctcggcttcg gcctgatcgc gctggtgccc gcattcttcg
     3481 actggacgcg catccgcgcg ctcacgcggg ccgactggat cgccgccctc aagctttcac
     3541 tggtcggcaa catcctgtac tacgctttcc tggccaccgc gatccagctg gccgacgcgc
     3601 cgctgccggc aatgatcatc ggcacgctgc cgctcgtgat ctcgatctgt tcgaactggt
     3661 cgcccggcca tgccggcgag acgattccat ggatgcggct cgcgccttcg ctgctgatca
     3721 tcgccgccgg cctgctgctg gtgaatgcgg gggaactggc gcaccttgat ggcgcgcgca
     3781 gcaccgccga ctacctgctc ggctgcacag tcgcgctcgg ggcgctggcg gcgtggacct
     3841 ggtacccgat ccgcaatgcc cgctatctga aggcgcatcc gcgcatccgc tcgtccacat
     3901 gggcgaccgc gcaagggctc gcgacgctgc cgctggccgc catcggcttt gccggttacg
     3961 gcatccatac ctggctgaac ggcggcggct acgcatttcc cgcggggccg cgcccgttcg
     4021 atttcatcgc gctcatgctc acactcggac tgtgcgcgtc gtggctcggc accctgctgt
     4081 ggaacaaggc cagccagaag ctgccgacct cgctggccgg acaactgatc gtcttcgaga
     4141 cgctggccgc cctgctgtat gcgttcctgt ggcgcggcgc gatgccggcc cccggcgtgc
     4201 tggccgggat tgccctgctg tgcgcaggcg tcgtgctggg gatgcgggca ttccgcaaca
     4261 cggccggtgc cgcaaccgaa gcggcgacat gatgcagacg cgttcccgcg tccttgctcg
     4321 aacggcaagt gcaattcgga tactgcgcca cgcgtgaata cggccgacct gctaccatcg
     4381 ccggatgcga cactccaccc tgcgccgcgc ggctgccgcg gcttgctgcg ttctgctcct
     4441 tgcctgcagc cccaagttcg actggcgcga agtgcatggc agcgccgccc cgtattcggc
     4501 gctgctgccg gccaagccgg ccacccatgc acgcgacatc aatcttgacg gccttcccgt
     4561 gaccatgacg atgaccggcg ccgaggtcga cagcgtcacc tacgcgatcg gcaccatcga
     4621 actggccgag cctgcacagg cagcgcacgc gctcaacgcg atgcgaaccg cgctggtgcg
     4681 caatatccac ggcaccgtca cgcaggaaaa gagctccgct cccgccgacg gcacgaccat
     4741 gcttgaactg gaggccaacg gcccgctgcc gcagggccgc cgcctgctgc aggcgcgcct
     4801 gatcgcgcgc gaacgtcacg tctaccaact gctcgtcgtc ggtgacgaac gcgacttctc
     4861 gcgtgaagcc gccgacacct tcttcacatc gttccgtctg tcctcgacca acaactagaa
     4921 caatgagctc cagcctgaaa ggggtactcg cgatccgcgt gttcctcagc ttcgcgtttg
     4981 cctacttcct gtcctatgcg ttccgctcgg tgaatgcagt gatcggcccg gaactgcgtg
     5041 tcgacctgtc tctgtcgaac gccgacctcg gcctgctgtc cgccgcctat ttcctcacct
     5101 tcgccgcgat gcagcttccg ctcggcgtct ggctggacaa gtacggtccg cgccgcaccg
     5161 aatccgcgtt gctgctggtg gcggccgccg gctcggccat cttcgcgatg agcgagaccg
     5221 cggcgggact ttggaccggc cgcgcgctga tcggggtcgg cgtttcgggc tgcctgatgg
     5281 cgccgttcaa ggcttatcgg ctctggtttc cggccgagcg ccaggcccag ctcgccagct
     5341 ggatgctggt ggccggcacg tccggagcac tggcgtcgac cgtgccggtg agcgccgcga
     5401 tgccgatcat cggctggcgc ggcgtgttct gggtgatgtg cgcaatgatc ctggccgcgt
     5461 cagctgcgat cttcttcctg ctcaagcgga tcgagcggga acatccggct cccgtggccg
     5521 cgccgtcgag caccccggtc gccggcgcca cctacgccag catcttcggc aacccgttct
     5581 tccgccgcat ggccatcctc ggcgcgatca accttgccag cttttcggcg ctgcaaacgc
     5641 tgtgggccgg gccgtggatg gtgacggtac tgggcatgga agcgcaccag agtgcgaatg
     5701 tgctgttcat gttcaacctg gtcctgctct gcgcctacct cgtgctcggc tggtgggcgc
     5761 cgcgtcacgt ctcatacgga ggcggcgccg gcatcccggc actgcgcgtg gtcgccatcg
     5821 gaatcggcct tgcgatcctg gtacagttcg ccatcattgc ctttaccggc acctggagct
     5881 ggatgctgtg gatcttgctg gcgttgtgcc tgacaatgaa cacgctggcc gccaccgccg
     5941 tcagcctgca tttccctccg gcatcggccg ggcgcgcgaa cagctcgttc aacctgaccc
     6001 agtttgccgg caccttcatc gtgcaatggg gaatcggcct gctgatcgac gcgttcggaa
     6061 cggcaggcgc cagcagcgtt gacgcgatgc ggctggcctt cgcggtgtgc gccggcgtgc
     6121 aggcgcttgc actggccgca ttcctgctcg ccggacgcag atccgcctga cccacggcaa
     6181 gacggggaac ccggaaacgt tttcaactgt ttgcctagac cgaatttcat tggaggtccc
     6241 atgctgatca cgttcaaatc gaaggccgcc gccgacatcc tgatgtacga gaagcatgcc
     6301 aagcccatcc tcgacctgct caaccgggac gtcgaacgcg gcatcatcac ggccgcggag
     6361 atgaacgacg cggtcgccag gctggaagcg gcaatcgagg aaagccgtcg acgcgaggcg
     6421 caggagcgcg cgcagcgcga agcggactcc gaagaggacg aagaggacga ggcgcaaccg
     6481 cagcaaacgg tgagcttcgc cactcgcgcc caccccctgc tggaaatgat gcgcgcagcc
     6541 gcccgcagcg gcgccaacat catgtggggc gtgtaacgca tccagactgt ttgcgggatg
     6601 accatggaca cgctaggtat cgtcaatttc ggcctgttcg ttgcggcggt cctgctgctc
     6661 aacgtcacgc ccggcccgga cacggcctat attgtcgggc gcagcctcgc gcaagggcgc
     6721 atggccggcc tggtgtcggc cctcggcatt tccgcgggct gctgcgtgca tgcgctggcg
     6781 ctggcgatcg ggctgagcgc gctgctgcag gcttccgccg gcgcgttcac cgcgatcaag
     6841 ctcgccggcg gcgcctacct tgcctggctc ggcctgcgca tgctgctgtc cagaccgcag
     6901 aacgccgcgg ccgcgccggt cgcgccggct cgcgcgatgt cgatgatttt catgcaggca
     6961 ctggcgacca atgtgctcaa tcccaaagtg atcctgttct tcctggcgtt catcccgcaa
     7021 ttcgtgcgcc cggatgcgcc gcacaaggcg ctcggctttc tcgtgctggg cgtggcgctc
     7081 gcgctgatca gcctggcctg gaacggcgcc accgcgctgc tggccggcac gctggcgcgc
     7141 ggcgcgggcc gcaacccgac ggtgcgtgtt tggcttgaac gggtggtcgg cgcaagcttc
     7201 attgcactgg gcgcgcggct tgcattgggg cgcagctgag atggccggac ccgcacgccc
     7261 gcgcacaccc gcgctgcgca ccgactcgct ggcctatgcg ctgtcgggcg ccgcgcacgc
     7321 cgttgcgatc gtggccgaag gctcggccct gccgcaggcg ctggcaagcg tcttccgccg
     7381 cctgcaggcg ccgccgcagg cgcgcggtgc gatccaggac atcgcctatc gcacgatgcg
     7441 ccagcgtgcc agttccgacg ccctgctcgc gcaactcagc gaccgcccgg tctccccccc
     7501 gacgctgcat gcgctgctcg cgtgctcgct cgcacttctg cagccggctg caggcgaaga
     7561 cccgggcagc ctgccgtacg aggcgttcac ggtggtggac caggccgttg ccgccgccgg
     7621 cgccaatccc gccctggccc gcgccaaggg catggtcaac gcggtcctga ggcgcttcct
     7681 gcgcgaacag gcagcactgc tgccggcgct ccagcacgac ccggtcacgc gctggaacca
     7741 tccgcgctgg tggatcgatg cattgcgcag cgcctatccc gattcctggc aggcgattct
     7801 cgaagccggc aaccggccgc cgccgctcac cctgcgcgtg aacctgcgcc gcgttacgcc
     7861 cgaggattat cttgcccgtc tggccgcggc gggaatcagt gcgcggcaga tcggcccgca
     7921 ggcggtccgg ctggaccgcg cgctgccggt gggccagatc cccgggttcg atgaaggcct
     7981 ggtttcggtg c
//
LOCUS       DATLGF010000041         5299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_33734,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000041 DATLGF010000000
VERSION     DATLGF010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5299
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33734"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /gene="bamD"
                     /locus_tag="VM406_01310"
     CDS             <1..315
                     /gene="bamD"
                     /locus_tag="VM406_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HVL74626.1"
                     /translation="YASDSIARMNYLVNSLAQYEVHVANYYYRRGAYLAAANRAQRAI
                     SEYREAPAIEEALYVLMRSYDALGMTQLRDDAARVMRATYPDSVYFRGGPQKSEPWWK
                     IW"
     gene            complement(357..1133)
                     /gene="yaaA"
                     /locus_tag="VM406_01315"
     CDS             complement(357..1133)
                     /gene="yaaA"
                     /locus_tag="VM406_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HVL74627.1"
                     /translation="MLIVISPAKTLDYDTAAQTDVQTMPEFLDDAAELVSVLRKLSPA
                     EIGSLMHISDPLALLNAGRYASWSSRFTKQNAKQAVLAFNGDVYEGLDAASLNAGQLD
                     YLQAHLRILSGLYGVLRPLDLMQPYRLEMGTRLATGRGKDLYAFWGARVTEALGAAMR
                     AAGANALVNLASEEYFKVVRPALLGVPVITPVFEDWKGGRYKIISFHAKRARGLMTRH
                     AALHGITDPRALKGFDAEGYAFDAAASGDTQWVFRRRLED"
     gene            1170..1598
                     /locus_tag="VM406_01320"
     CDS             1170..1598
                     /locus_tag="VM406_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HVL74628.1"
                     /translation="MIPKRIVLDTNICLDLFVFCDPRWNALLAALESGAVQAVTRADC
                     RTEWIRVLDYPHLPLDDHSRAQAMSRFDALIPVVPSEPAGTAPLPLCKDPDDQKFLEL
                     ARDAGAAQLITKDKALLKLARKTARAGMFEILPPERWQAE"
     gene            1656..2837
                     /locus_tag="VM406_01325"
     CDS             1656..2837
                     /locus_tag="VM406_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HVL74629.1"
                     /translation="MNAPLTSAEFTPALQSRLPNVGTTIFTVMSALAVEKGAVNLGQG
                     FPDFDCDPALVDAVAEAMKAGLNQYPPMAGVPALREAIAAKIQTLYGRRYDPAAEITV
                     TAGATQALVTAILCAVHPGDEVIVIEPVYDSYAPSIELAGGKVVPVQMEVGAQGYAIP
                     WDKVAAAVSARTRLIMINSPHNPTGSVLRQSDMQALADIVRGTDILLVSDEVYEHMVY
                     DGQPHESVCRHPELAARAFVVSSFGKTYHVTGWKVGYVAAPAALTAEFRKVHQFNVFT
                     VNTPVQHALAQYMRNPAPYLELPAFYQRKRDLFRSRLQASRFKLLPSDGTYFQCVDYS
                     AISDLPEADFARWLTTEIGVAAIPVSAFYSRPKESGVVRFCFAKKDETLNAALDLLTR
                     V"
     gene            2935..4086
                     /locus_tag="VM406_01330"
     CDS             2935..4086
                     /locus_tag="VM406_01330"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HVL74630.1"
                     /translation="MEEQRRISLNDLVVGEPLPWDVYGTGNKLLLRRGHVLDSARQAE
                     DLVARGVFIDGGQAERDRQARLDALEAARREAPSALRLINESVARLGRLLYNLGNEAD
                     PGSRIAEIGAAINAALDINTDVALASIQLNRESGTYAVRHAVDTAILALLVARAMRRD
                     EAERRSLVAAALTMNVGMLRFQDHLQGKVEPLSEAERADVRGHTEESARLLAAAGIDD
                     PLWLACVQMHHDDPDGSGYPLGARVTDIPEAARILAMADRYCAAVSLRAYRKTLLPGP
                     ALRDVLMRGGKTSEPVLAAHLIKELGTSPPGTLVRLADGEIGVVTRRASSTSAPVVHA
                     FIGPRGSPLSFPIKRDTGKPLHAIREVLPSDHAVLRFPLQQLWGSEAAP"
     gene            complement(4087..4737)
                     /locus_tag="VM406_01335"
     CDS             complement(4087..4737)
                     /locus_tag="VM406_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HVL74631.1"
                     /translation="MIKLCGFAVSNYYNKVKLALLEKGVPFEEELVWTDRSPALLARS
                     PLGKVPFMETPHGRLCESQVLIEYIEHAYPEKPLLPKDPFAAAKVRELVTFMELHLEL
                     VAREVYAEAFFGGTVSDEVKARTQKLLSRNGKAFGKLAKFSPYVAGDAFTIADCAALV
                     HLPLVSMASKKLFGEDVLAEYPVRDYIKMLGERETVQKVNADRKANQELMAARAKQ"
     gene            4890..>5299
                     /locus_tag="VM406_01340"
     CDS             4890..>5299
                     /locus_tag="VM406_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVL74632.1"
                     /translation="MSAELIAQRRDATLVLTISNPGARNALHPDMYAAAIETLTTAER
                     DDSLRAVVLTGADQYFCAGGNLRRLLENRSKDPSEQAQSIDQLHGWIEAIRDCPKPVI
                     AAVEGGAAGAGCSLALACDFIVAGQSAKFAMSYV"
BASE COUNT          942 a         1813 c         1621 g          923 t
ORIGIN      
        1 tatgcgagcg actccatcgc gcgcatgaac tacctggtca attcgctcgc ccagtacgaa
       61 gtgcatgtcg ccaactacta ctaccgccgc ggcgcctatc tggctgcggc caaccgcgcc
      121 cagcgcgcaa tttccgaata ccgcgaggcg ccggccatcg aagaggcgct gtatgtgctg
      181 atgcgttcct acgatgcgct gggcatgacc caactacgcg acgatgcagc acgcgtgatg
      241 cgggccacct accccgacag cgtctacttc cgcggtgggc cgcagaagag cgagccctgg
      301 tggaagatct ggtagcgtaa gaaagcctcg gaagaaccga ggcttttttg tttcattcaa
      361 tcctcgaggc gtcgccggaa gacccattgc gtatcgccgg acgccgcggc atcaaacgcg
      421 tagccttcgg cgtcaaaccc cttgagcgcc cgcggatcgg tgatgccgtg cagcgccgca
      481 tgacgcgtca tcagaccgcg cgcgcgcttc gcatggaagg aaatgatctt gtacctgccg
      541 cccttccagt cctcgaacac cggcgtgatc accgggacgc cgagcagcgc cggcctgacc
      601 accttgaagt actcttcgga agccaggttc accagggcat tggcgccggc cgctcgcatc
      661 gccgcgccga gcgcttccgt cacgcgcgcg ccccagaatg catacaggtc cttgccgcgg
      721 ccggtggcca ggcgcgttcc catttccagc cggtacggct gcatcaggtc cagcggccgc
      781 agcacgccgt acaggccgga caggatgcgc aagtgcgcct gcaggtaatc gagctggccc
      841 gcattcagcg aagcggcatc caggccttcg tacacgtcgc cgttgaatgc cagcacggcc
      901 tgtttcgcat tctgcttcgt gaaacgactg gaccagcttg catagcgtcc ggcgttcagc
      961 aatgcaagcg gatcggaaat gtgcatgagg ctgccgatct cggccgggga cagcttgcgc
     1021 agcaccgaca ccagttccgc cgcatcgtcg aggaactccg gcatcgtttg cacatcggtc
     1081 tgcgcagctg tgtcatagtc gagcgttttc gctggggaga tcactatcaa catgtcgaac
     1141 cggcctgctt ctgtttccaa gaacccgcca tgatacccaa gcgcatcgtt ctcgacacca
     1201 acatctgcct ggacctgttc gtcttctgcg atccgcgctg gaacgcgctg ctcgccgcgt
     1261 tggagtcagg cgccgtgcag gcggtgacgc gcgcggactg ccgcaccgaa tggatcaggg
     1321 tgctcgacta tccgcatctt ccgctcgacg accacagccg cgcccaggcg atgtcccgct
     1381 tcgacgcgct gattccggtc gtgccgagcg agcctgccgg cactgcgccg cttccgctct
     1441 gcaaggatcc ggacgaccag aaattcctcg agctcgcccg cgacgccggc gcggcacagt
     1501 tgatcacgaa ggacaaggcc ctgctcaagc ttgcgcgcaa gaccgcacgt gccggcatgt
     1561 tcgagatcct gccgcccgaa cgctggcagg cggaatgacc cgcgcgtagc cgtcgttgga
     1621 tacaatggtc gaacctgtct gaaccgctcg atacgatgaa cgcaccgctg acctccgccg
     1681 aattcactcc cgccctgcaa tcgcgcctgc ccaacgtcgg cacgacgatc ttcaccgtca
     1741 tgtcggcgct ggcggtggaa aaaggcgcgg tcaatctcgg ccagggcttt ccggatttcg
     1801 attgcgaccc ggcgctggtg gacgcggtcg ccgaagcgat gaaggccggg ctgaaccagt
     1861 atccgccgat ggccggcgtg ccggcgctgc gcgaggcgat cgccgccaag atccagacgc
     1921 tgtacggccg ccgctacgat cccgcggccg agatcaccgt gacggccggc gccacgcaag
     1981 cgctggtgac cgccatcctg tgcgccgtgc atcccggcga cgaagtcatc gtgatcgagc
     2041 cggtctatga cagctacgcg ccgtcgatcg agctggccgg cggcaaggtg gtgccggtgc
     2101 agatggaggt gggggcacag ggctatgcca tcccctggga caaggtcgcg gccgccgtgt
     2161 ccgcccggac ccgtctcatc atgattaact ccccgcacaa ccccaccggt tcggtgctgc
     2221 ggcaaagcga catgcaggcg ctcgccgata ttgtgcgcgg caccgacatc ctgctggtgt
     2281 cggacgaggt ctacgagcac atggtctacg acggccagcc gcacgaatca gtgtgccgcc
     2341 atccggaact ggccgcgcgc gccttcgtgg tgtccagctt cggcaagacc tatcacgtga
     2401 ccggctggaa ggtcggctac gtcgcggcgc cggccgcgct cacggccgag ttccgcaagg
     2461 tgcaccagtt caacgtgttt acggtcaata cgccggtcca acacgctttg gcgcagtaca
     2521 tgcgcaatcc cgccccctac ctggaactgc cggcgttcta ccaaagaaag cgcgacctgt
     2581 tccgcagccg cctgcaggcc tcccgcttca agctgcttcc atccgacggc acctacttcc
     2641 agtgcgtcga ctactcggcg atctcggatc tgcccgaggc cgatttcgcg cgctggctca
     2701 ctaccgaaat cggcgtggcc gccattcccg tttccgcgtt ctacagccgg ccgaaggagt
     2761 cgggcgtcgt gcgcttttgc tttgcgaaga aggacgaaac cctgaacgcg gcgctggacc
     2821 tgctcacaag agtctagggc cgttcaccgg ccctcaagct tggcacttcc ttcgccgtaa
     2881 acctgatcaa gggcgcgcac gcccgcgcgt ccggttgcgc atggagggag tgccatggaa
     2941 gagcaacgtc gcatcagcct gaatgacctg gtggtcgggg aacctctgcc ttgggatgtc
     3001 tacggcacag ggaacaagct gctgctacgg cgcggccacg tgctcgacag cgccaggcag
     3061 gcggaggatc tcgtggcgcg cggggtgttc atcgacggcg ggcaggccga gcgcgatcga
     3121 caggcccgcc tggacgcgct ggaagcagcc aggcgcgaag cgccctcggc actgcgcctg
     3181 atcaacgagt ctgtcgcgcg cttgggccgc ctgctgtaca acctgggcaa tgaagccgat
     3241 cccgggtcac gcatcgcgga aatcggcgcg gcgataaacg ctgcgctcga catcaacacc
     3301 gacgtcgcgc tggcctcgat ccagctcaat cgcgagtccg gcacctacgc ggtacgccat
     3361 gcggtcgaca cggccatcct ggccctgctg gtcgcgcgcg cgatgcgaag ggacgaagcg
     3421 gaacgccgca gcctcgtcgc cgccgcgctg acgatgaacg tcggcatgct gcgctttcag
     3481 gaccatcttc agggcaaggt ggagccgctc tccgaggcgg aacgggccga cgtgcgcggc
     3541 cataccgagg aaagcgcgcg gctgctggcg gcagccggca ttgatgatcc gctgtggctg
     3601 gcctgcgtgc agatgcacca cgacgatccc gatggaagcg gctatccgct tggcgcgcgt
     3661 gtcaccgaca tcccggaagc ggcacgtatc ctcgcgatgg cggaccgcta ttgcgcggcc
     3721 gtctcgctgc gcgcctaccg caagacccta ctgcccggac ccgcgctgcg cgatgtgctg
     3781 atgcggggcg ggaaaacctc ggaaccggtg ctagcggccc atctgatcaa ggaattggga
     3841 acctcgcctc ccggcacgtt ggtccggctc gcggatggcg agatcggcgt cgtgacgcgg
     3901 cgcgcaagtt cgacgtccgc accggtcgtt catgctttca tcggcccgcg cggctcgccg
     3961 ttgtcgttcc ctatcaaacg ggataccggc aagccgctgc acgccatccg cgaagtgctg
     4021 ccttcggacc acgccgttct tcgatttccg ctgcagcagc tttggggcag cgaagccgcg
     4081 ccctgactat tgcttggcgc gcgcggccat cagttcctgg ttggccttgc gatcggcgtt
     4141 gaccttctgg accgtctcac gctcgcccag catcttgatg tagtcgcgca ccggatactc
     4201 ggccagcacg tcctcgccga acagcttctt cgaggccatc gacaccagcg gcaagtgcac
     4261 gagcgccgcg cagtcagcga tcgtgaacgc atccccggcg acatacggcg agaacttcgc
     4321 cagcttgccg aaggccttcc cgttacggct caacagcttc tgcgtacgcg ctttcacctc
     4381 gtccgacacc gttccgccga agaaggcctc cgcatagact tcccgagcga ccagctcgag
     4441 atgcagctcc atgaacgtga ccagctcacg cacctttgcc gccgcaaacg ggtccttcgg
     4501 cagcagcggc ttttccggat acgcgtgctc gatgtactcg atcagcacct gcgattcgca
     4561 cagcctgccg tgcggcgttt ccatgaacgg caccttgccc agcggcgagc gcgccagcag
     4621 cgcgggcgag cggtcggtcc acaccagctc ctcctcgaag ggcacgccct tttccagcaa
     4681 cgccagcttg accttgttgt agtaattgct gaccgcgaaa ccgcagagct tgatcatgat
     4741 gttgtctcct cttgttgaat ggcgccatac actctatccg aactgcaatg cgtgcgcttt
     4801 tgcaccttcc ataacgattt tgcggaatcg gcaataatag gcacttgccc ctgcgggcat
     4861 ttaccaccgt cccactcacc ttactcgcca tgtccgccga actgattgcc cagcgccgcg
     4921 acgccacgct cgtcctgaca atatcgaacc cgggggcgcg caacgcgctg catcccgaca
     4981 tgtacgccgc cgccatcgag accctgacca ccgccgagcg cgatgacagc ctgcgcgcgg
     5041 tggtgctgac cggagccgac cagtatttct gcgcaggcgg caatctgcgc cggctgctgg
     5101 agaaccgcag caaagacccg agcgaacagg cgcagtcgat cgaccagctg cacggatgga
     5161 tcgaagcgat acgcgattgc ccgaaaccgg tgatcgccgc ggtcgaaggc ggcgcggccg
     5221 gcgccggatg ctcgctcgcg ctcgcctgcg atttcatcgt cgccggccaa tccgcaaaat
     5281 ttgcgatgag ctatgtgaa
//
LOCUS       DATLGF010000042         4405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_222769, whole genome shotgun sequence.
ACCESSION   DATLGF010000042 DATLGF010000000
VERSION     DATLGF010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222769"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /gene="hflK"
                     /locus_tag="VM406_01345"
     CDS             <1..887
                     /gene="hflK"
                     /locus_tag="VM406_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HVL74633.1"
                     /translation="PYPIQSHETVNVDTVRTVEVGYRNHVRNKQPRESLMLTDDANII
                     DIQFAVQYRLTNAADWLFNNREQEEMVKQVAETSIREVVGRSKMDFVLYEGREQVAFD
                     VNQLMQQIVDRYNSGVLITNVTLQTVQPPEQVQAAFDDAVKAGQDRERQKNEGQAYAN
                     DVIPRARGAASRLLQEAEAYRSRVVATAEGDADRFRQVLFEYQKAPAVTRERMYLETM
                     QQIFANTTKVMVDTRNQSNLLYLPLDKLIAQTAGEAARPQPAPGAHSVDPAQLEAQQR
                     QRDQRSREGRDAREREAR"
     gene            887..1783
                     /gene="hflC"
                     /locus_tag="VM406_01350"
     CDS             887..1783
                     /gene="hflC"
                     /locus_tag="VM406_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HVL74634.1"
                     /translation="MNRLFGYVIAALILLMVAMSTLFVVDQRKFAIVFALGEVRSVVD
                     EPGLHFKLPPPFQNVLFLDKRILTLDTPEADRFITAEKMNILVDSYVKWRIIEPRLYF
                     VSFTGDERRAQDRLAQIARAALNEEITRRTVGAVISAERAEVMNAVQRKVTEEAKRIG
                     IGIVDVRLKRVEYVEQISTSVYDRMRAERVRVANELRSTGAAESEMIRADADRQRTVI
                     LAEAYRDAEKIRGDGDAKATNIYGQAFGQNPEFARFYRSLEAYRASFRDRSDMMVLDP
                     SSEFFRYFKNATPGAQAPAAPR"
     gene            1826..1990
                     /locus_tag="VM406_01355"
     CDS             1826..1990
                     /locus_tag="VM406_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HVL74635.1"
                     /translation="MMLLLEGIIPFLFPRQWRAAFSRIMGLSDGQIRFLGLFALICGL
                     VLIGLVRAFS"
     gene            1998..3149
                     /locus_tag="VM406_01360"
     CDS             1998..3149
                     /locus_tag="VM406_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HVL74636.1"
                     /translation="MPAWLLPENIADVLPSEARKIEELRRAILDNFRLYGYELVMPPM
                     LEYVESLLTGAGQDMDLRLFKLVDQLSGRTMGVRADMTTQVARIDAHLLNRNSVTRLC
                     YAGSVLHARPSGLYATREPFQIGAEIYGHAGLEADAEVQALALESLRLAGIAQVRLDL
                     CHVGVLRAIIAGDAAARHNEAHLFELLQAKDVPGLREVTRDYAPRTRSALLALPGLYG
                     GPEVIERARMMLDDVSGVGQALDELAHLIAQIDGADVTVDLADLRGYHYHSGVMFAAY
                     VPGLPNALARGGRYDEVGEAFGRARPATGFSMDLRELARLMPGAARKQAVRAPWSTDA
                     ALRAKVAELRRAGEIVIQAMPGHENDHDEFDCDRMLVCEHGNWILKTLG"
     gene            3155..>4405
                     /locus_tag="VM406_01365"
     CDS             3155..>4405
                     /locus_tag="VM406_01365"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HVL74637.1"
                     /translation="MAKNVVVIGTQWGDEGKGKVVDWLTDHAQGVVRFQGGHNAGHTL
                     VIGGQKTALQLIPSGIMRAGVACYIGNGVVLSVPDLLREIDKLEANGVEVASRLKVSE
                     ACPVILPYHTALDAAREAARGAAKIGTTGKGIGPAYEDKVARRAIRVADLLSEARFAE
                     KLRENLDYHNFVLTQYLKADAVDYQKTYDDAMAMVPRLRPMVTDVSSALYAAHKAGSS
                     LLFEGAQGSMLDVDHGTYPFVTSSNCVAGNASSGTGVGPNMLHYVLGITKAYTTRVGS
                     GPFPSELPTDAGIGKHLASVGHEFGTVTGRARRCGWFDAALLRRSVQINGVSGMCLTK
                     LDVLDGIETLKLCTGYTLDGRNVDIFPVGAEDAARCQPVYEEMPGWSESTVGAKSLAA
                     LPATARAYIKRIEELVGVPIDMVST"
BASE COUNT          792 a         1394 c         1453 g          766 t
ORIGIN      
        1 ggccgtatcc gatccagagc cacgagacgg ttaacgtcga taccgttcgc acggtcgagg
       61 tcggatatcg caaccatgtc cgcaacaagc agccacgcga gtcgctgatg ctgaccgacg
      121 acgcgaacat catcgacatc cagttcgcgg ttcagtaccg cctgaccaac gccgctgact
      181 ggttgttcaa caatcgcgag caggaagaga tggtcaagca ggtggccgag acctcgattc
      241 gcgaagtggt cggccgcagc aagatggact tcgtgctcta cgagggccgc gagcaggtgg
      301 cgttcgatgt caatcaactg atgcagcaga tcgtggaccg ctacaactcc ggtgtgctga
      361 tcacgaacgt gaccctgcag accgtacagc cgcccgagca ggtgcaggcg gcgtttgacg
      421 acgcggtcaa ggccggtcag gaccgcgagc gccagaagaa tgaaggccag gcgtatgcga
      481 acgacgtgat cccgcgtgcg cgtggtgccg cgtcgcgtct gttgcaggaa gccgaggcct
      541 accgctcccg cgtggttgcc accgcagagg gtgacgcaga ccgcttccgc caggtgctgt
      601 tcgagtacca gaaggcaccg gcggtcacgc gcgagcggat gtatctcgaa acgatgcagc
      661 agatttttgc aaacaccacc aaggtcatgg tcgacacccg caaccagagc aatctgctct
      721 atctgccgct agacaagctg attgcccaga cggccggcga ggctgcgcgt ccccagcccg
      781 cgcccggcgc acattctgtc gatccggcgc agctggaagc gcagcagcgc cagcgtgacc
      841 agcgcagtcg cgaaggccgc gatgcgcgtg aacgggaggc acgctgatga accgcctctt
      901 cggttatgtg attgccgccc tgatcctgct catggtcgcg atgtccacgc tgttcgtcgt
      961 ggatcagcgc aagttcgcga tcgtgttcgc cctgggcgag gtgcgcagtg tcgtcgatga
     1021 acccggcctg catttcaaac tcccgccgcc tttccagaac gtgctgttcc ttgacaagcg
     1081 catcctgacg ctggatacgc cggaagcgga ccgcttcatc acggccgaga agatgaatat
     1141 cctggtcgac tcgtatgtga agtggcgcat catcgagccg cgcctgtact tcgtgtcctt
     1201 caccggcgac gagcgccgtg cccaggatcg cctggcgcag atcgccaggg cggcgctgaa
     1261 cgaggaaatc acgcggcgca cggtgggcgc ggtgatctcg gccgagcgcg cggaggtcat
     1321 gaacgccgtc cagcggaaag tgaccgagga agccaagcgc atcggcatcg gcatcgtcga
     1381 cgtgcggctg aagcgcgtcg agtacgtcga gcagatcagc acctcggtct atgaccgcat
     1441 gcgtgcggag cgtgtgcgcg tggccaacga gctgcgttcg accggcgcgg ccgaatcgga
     1501 aatgatccgt gcggatgcgg accgccagcg aaccgtgatc ctcgccgaag cctatcgcga
     1561 cgccgagaag atccgcggtg acggcgatgc gaaggcgacc aacatctacg gtcaggcgtt
     1621 cggccaaaat ccggagttcg cgcgcttcta ccgcagcctg gaagcctatc gtgcgagctt
     1681 ccgcgatcgt agcgacatga tggtgctcga tccgagttcg gagttcttcc gctacttcaa
     1741 gaacgcaacg cctggcgccc aggcgcccgc cgctccccgc tgatgcggat actgtgtgaa
     1801 cggctcctgg accattgcgc tcggaatgat gctgctcctc gagggcatca ttccgtttct
     1861 ctttccgcgg caatggcgcg ccgcctttag ccgtatcatg ggcctgtccg acggacagat
     1921 ccgctttctc ggcctatttg cactgatttg cggtcttgtc ctgatcggac tggtccgcgc
     1981 cttttcctga tcgcgttatg cctgcctggc tacttcccga gaatattgcc gacgtcttgc
     2041 cgtccgaggc gcgcaagatc gaagaactgc gccgcgcgat actcgacaac ttccgcctgt
     2101 acggctatga gctggtcatg ccgccgatgc tcgagtatgt cgagtcgctg ctgaccggtg
     2161 ccggccagga catggacctg cgcctgttca agctggtcga ccagctctcg ggccgcacga
     2221 tgggcgtgcg cgcggacatg acgacccagg tcgcgcgcat cgacgcgcat ttactcaatc
     2281 gcaactcggt cacgcgactc tgctatgcgg gcagcgtgtt gcacgcgcgc ccgtcgggcc
     2341 tgtacgcgac gcgggagccg ttccagatcg gtgcggaaat ctatggccat gccgggctgg
     2401 aagccgatgc agaggtccag gcgcttgcgc tcgagtccct caggctggcc ggcattgccc
     2461 aggttcggct ggacctgtgt catgtcgggg tgctgcgggc gatcattgcc ggcgatgcgg
     2521 cggcccggca caacgaagcg cacctgttcg aattgctcca ggccaaggat gtgccgggcc
     2581 tgcgcgaggt gacgcgcgat tacgcgccgc gcacccgcag cgcgctcctt gcgctgccgg
     2641 gcctttacgg cggaccggaa gtcatcgagc gggcgcgcat gatgcttgac gatgtgtccg
     2701 gcgtgggcca ggcattggat gaactggcgc acctgattgc ccagatcgat ggcgcggacg
     2761 tgacggtcga tctggccgat ctgcgcggct accattacca cagcggcgtg atgttcgccg
     2821 cgtacgtgcc ggggctgccg aatgcattgg cgcggggcgg gcgctacgac gaagtcggcg
     2881 aggcattcgg ccgcgcacgc ccggcgaccg gcttttcgat ggacttgcgc gaactcgctc
     2941 gcctgatgcc gggcgccgcg cgcaagcagg cggtgcgggc accatggagc accgacgccg
     3001 cgctgcgcgc gaaggttgcg gaactgcgcc gggctggcga aatcgtgatc caggcgatgc
     3061 ccggacacga gaatgatcac gacgagttcg actgcgatcg catgctcgtc tgcgaacacg
     3121 gaaactggat tcttaaaaca ctaggctaat gagaatggca aagaatgtgg ttgtcatcgg
     3181 cacccaatgg ggcgatgaag gcaagggcaa ggtcgtcgac tggctgaccg atcatgcaca
     3241 gggcgtcgtg cgctttcagg gcgggcacaa cgccggccac acgctggtga tcggcgggca
     3301 gaagacggca ttgcagctga ttccgtccgg catcatgcgc gccggcgtcg cctgctacat
     3361 cggcaatggc gtggtgctgt cggtgccgga tctgctgcgc gagatcgaca agctcgaggc
     3421 caacggcgtc gaggtcgcct cgcgcctgaa ggtgtccgag gcctgcccgg tgatcctgcc
     3481 gtatcacacg gcgctcgatg ccgcgcgcga ggctgcacgc ggcgcggcca agatcggcac
     3541 caccggcaag ggcatcggcc cggcctacga agacaaggtc gcgcggcgcg cgatccgcgt
     3601 cgcggacctg ctcagcgagg cgcgctttgc cgagaagctg cgggagaacc tcgactacca
     3661 caacttcgtg ctcacgcaat acctgaaggc cgacgcggtc gactaccaaa agacctacga
     3721 cgatgcgatg gcgatggtgc cgcgcctgcg cccgatggtc accgacgttt cgtcggcact
     3781 gtatgccgcg cacaaggcgg gcagcagcct gttgttcgaa ggcgcgcagg gtagcatgct
     3841 cgatgtcgac cacggcacct atccgttcgt gacttcgagc aactgcgttg ccggcaatgc
     3901 atccagcggc acgggcgtcg gaccgaacat gctgcactat gtcctcggca ttaccaaggc
     3961 ctacaccaca cgcgtcggtt cgggcccgtt cccgtccgaa ctgcccaccg atgccggcat
     4021 cggcaagcac ctggcttccg tcggccacga gttcggtacc gtcaccggtc gcgcgcgccg
     4081 ctgcggctgg ttcgatgccg cgttgctgcg ccgctcggtg cagatcaacg gcgtgtccgg
     4141 tatgtgcctg accaagctcg acgttctaga cggcatcgag acgctgaagc tgtgcaccgg
     4201 ctacacgctg gacggtcgca acgtcgacat cttcccggtc ggcgcggaag atgcggcgcg
     4261 ctgccagccg gtgtacgagg aaatgccggg ctggagcgaa tcgacggtcg gcgctaagtc
     4321 gctggccgcg ctgccggcca ctgcgcgcgc gtacatcaag cgcatcgagg aactggtcgg
     4381 ggtgccgatc gacatggtgt cgacc
//
LOCUS       DATLGF010000043        11526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_966,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000043 DATLGF010000000
VERSION     DATLGF010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 11526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11526
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="VM406_01370"
     CDS             complement(<1..1035)
                     /locus_tag="VM406_01370"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HVL74638.1"
                     /translation="MRAMTPSSFASPVADLWRRTDRVLLAIAAILFVLVLLDPRQAQE
                     SLLFLGRALGAIAPILALSVALAAGIKAAGAEMLVSRAFAARQGRAILVAAIAGALSP
                     LCSCGVVPLIAGLLAAGVPLAPVMAFWLASPVMDPVMFVLTSGAIGVEFAIAKTAAAI
                     FMGVAGGWITRALTRSALLEQPLRATDAELDTRRLARLDAMRAAPVQWRFWTEADRAQ
                     VFLLSATRNAWTLGRWLALAFVLESLMLAWLPAEAVARTLGDSGGMAIPLAVLLGIPA
                     YLNGLAAIPLVGGLIGMGMNPAVGLAFMVAGGVTSIPAAMAVWALVRPRVFVLYVGLA
                     LLLALASAWLY"
     gene            complement(1110..1415)
                     /locus_tag="VM406_01375"
     CDS             complement(1110..1415)
                     /locus_tag="VM406_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HVL74639.1"
                     /translation="MNIDEVARCLSELGNTVRLEIFRLLVRAGDEGLSISEIRNAIGI
                     PASTLGFHLRGLVEAGLVLQERRGREVRCRANYPRLRGAIDFVMEECCKGAAVVQEL"
     gene            complement(1513..4212)
                     /locus_tag="VM406_01380"
     CDS             complement(1513..4212)
                     /locus_tag="VM406_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HVL74640.1"
                     /translation="MVESEDARAETKDEPARQRKKNDGKGAKRSWRRTVLRVALASVG
                     VFVVLLVGAGVFVANYLHGLNKTLPGVDAMLQARGAEPSILLSASGTHLATFSRGHFA
                     RVSLDEVSPYVVQALIAVEDHRFYEHKGIDYERTLGALWYTLRGNMQGGSTLTQQLAR
                     NMFPVRVGRERTLERKLREMLTALKIEQTYTKDEILETYLNSVPFLYQVTGIEMAART
                     YFDKSAADLDLLESATLVGMLKGTSYYNPVLHPERAQRRRNIVLQQMVRHGMLDAAVL
                     QRLRNEPLNVQLTRQPDPLGSAPHFAAHVRRQLIEWAEANDYNIYTDGLIVHSTIDDR
                     FQQAAAAAVERQTKLLQDIADVEWAQRDARVASQSPAHYTKLRKKIEPFSRFWEERPG
                     LLEAFVRESREFRRAQNSGESAEAALQRLLGDERFIARLREFKTRLEAGFVAIDPRSG
                     EVKAWIGSRNFEIDQFDHVAQAVRQPGSTFKPFVYGAALEHGLRPELQYPDGKIEIRL
                     SDGGVWRPTDMSGFSNRLMSMREGLVFSKNTITAQVMLDVGVEQVARLAKAVGIRSSR
                     LRLVPSLSLGTSPVTLLEMVSSYATIAQTGEYHAPLTIKRITDREGKVIAEFSRTPEY
                     VMSEETALELIDMMRGVVRQGTGTEVRNRFNITADIAGKTGTTQYNTDGWFILMHPEL
                     VAGAWVGFNDQRVTMRSDYWGQGGRNAVLLVGDFFRDTLRAKLIDAKARFPQANRPPP
                     LMVRAPLDEGAHVVQGIDLLPDGHGYITSSNGETMVILTPDGHHEVERRASAPPSAPE
                     QRAEATDAPVEPPPVAAAPDRAASASADGVGERSSGQSGTEGTSRSSEPVAQAPTVAP
                     PASPPPSGAAAAPATASAEQQRSARRNLQFRAGERWNGEIVPH"
     gene            4354..5490
                     /locus_tag="VM406_01385"
     CDS             4354..5490
                     /locus_tag="VM406_01385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVL74641.1"
                     /translation="MHLSFWEHDAAPDADFIVVGAGLIGLQTALELRALAPDARIAVL
                     ERGVLPYGASTRNAGFACFGSLTEILHDFDTMGEDAALAVVEQRWRGLARLRARVGEA
                     ALGFEPHGGYELLSTAQLPALERMDEANARLRPLFGRDVFQLDAAMRAASGFGPQVAA
                     LVANPLEGQLHSGRMMRALSRLAVDAGIALYGGVTVRSLHEGAAGVELHAGSAADPRE
                     LRFRAARVALCTNGLLGELAPACAVRPGRGQVLVTAPVAGLRWRGTFHMDEGYWYFRN
                     IGDRVLLGGGRHLDFAGEETSAMGLSAVIQQAQERLLRETILPGSKVAIEHRWSGIMG
                     FSADKQPIVRAVSPRVAIGFGCNGMGVALGADIAARTAALLHGC"
     gene            5783..6217
                     /locus_tag="VM406_01390"
     CDS             5783..6217
                     /locus_tag="VM406_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74642.1"
                     /translation="MPNILSCYYLDDPLTDEELAFVARTLTGYWARFKTGAQEVHQVR
                     VPGVLPLPDENGEYADSREERAQRVRHNLRHAGIRGDAGRRVVWVMPKDADWDAVFQF
                     AIREETGFAPFVAQRWFPKDGEQVRGSLRVIDTDMLLRGLES"
     gene            6228..6686
                     /locus_tag="VM406_01395"
     CDS             6228..6686
                     /locus_tag="VM406_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVL74643.1"
                     /translation="MSVKPIPEGYRNITPYLTIRNAAAAIDFYKQAFGAEERFRMSEP
                     DGRVGHAEIQIGDSLLMLSDEYPEMQATGPQTIGGTPVMMHLYVEDVDAVVKRAVDAG
                     AQLVRPVENQFYGDRAGLLQDPFGHKWWVSTHVEDVSPDELERRRQTAHA"
     gene            6785..7216
                     /locus_tag="VM406_01400"
     CDS             6785..7216
                     /locus_tag="VM406_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74644.1"
                     /translation="MASIQQQDGQDRQKKGAASGYAAGSEGWDSSEDFSDLPASSQAA
                     PPPQSDQETQSEFALSRASDDDEQDGRFSVAEEQNFDQQSDGARRVGQVPADAGAAGL
                     AGADAGDAMSESLQQDTQLEGEGQVPDSLKQAIERSVPPDG"
     gene            7233..7478
                     /locus_tag="VM406_01405"
     CDS             7233..7478
                     /locus_tag="VM406_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74645.1"
                     /translation="MLFYRRQAHVMPYVMELSGRLSILFSIGNSAGAENIMGRSLYNR
                     IQLGRRRLLLNMRRVRAAAGFLALCTGIVVWMIATYE"
     gene            7643..8584
                     /locus_tag="VM406_01410"
     CDS             7643..8584
                     /locus_tag="VM406_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYG1 family protein"
                     /protein_id="HVL74646.1"
                     /translation="MIIATHSGKFHADDVWAVAVLDIVFPGCELIRTRDPALIERADF
                     AVDVGGVWDPAAGRFDHHQKGFDAARASGVVYASAGLVWKAHGARCVARIAEREAGVQ
                     LDDERASQIAYAIDADVVQYLDMSDTGAARSAPGGYGLSALISGYNPNWMDEQHAGNA
                     AAAEALRLARFRRAMAVMEDVIVNQVKYRVGSLRALDQVRNGELLEGGRVLFLANAGL
                     PWSGVVRNEMPKVLLVIGYSIGDDRYMLSTVPANPETFEARMDLPAPWAGLREAELAA
                     ETGVPDAVFCHNKRFIAAARSFEGALALARIALRDAR"
     gene            8619..8942
                     /locus_tag="VM406_01415"
     CDS             8619..8942
                     /locus_tag="VM406_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HVL74647.1"
                     /translation="MGMSTTFLTEEPPRGEVDTLPGATVLEFGTGWCGFCRAAQPLLA
                     EAFDSHPQVRHLKIEDGRGRPLGRSFRVKLWPTLVFLRDGQEIARLVRPDSAAEIRDA
                     LQRIA"
     gene            complement(9053..9475)
                     /locus_tag="VM406_01420"
     CDS             complement(9053..9475)
                     /locus_tag="VM406_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74648.1"
                     /translation="MSAVLHPHPAALLQSAETRHLEGSLLERLGARAGLSPALASLLP
                     AFVSGAFAGAVVALALGGSFSQWLENWLVAWAVAFPLFYLAGTPLLRAVRFVSTPAKP
                     EAAALGIADIASASARASQRHGFTVLRNLKVREDFYRA"
     gene            complement(9501..9797)
                     /locus_tag="VM406_01425"
     CDS             complement(9501..9797)
                     /locus_tag="VM406_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74649.1"
                     /translation="MSLISALVPAEAVLANAGAVARPLLGLGLLTALVIFFRPFLAGL
                     LRAALMVLAPRTASALHAAREKAAGVMMLHRMAREVERNQPALAAELRAIASRD"
     gene            9956..10918
                     /locus_tag="VM406_01430"
     CDS             9956..10918
                     /locus_tag="VM406_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL74650.1"
                     /translation="MRFLTLDLNLLRVFDAVMTEQNLTRAASRLAMTQPAVSNALRRL
                     REALGDELLIRTAHGVKPTPRAETLWPAVRRALTELEEAITPEENFDIARAENTFRLA
                     MADATAALWLPSLVRSIEREAPLLTIRMVPLTTREPRPMLLRGDIDLAVGFFPGVVAQ
                     LAGGQVASVSPIRHEQLYSGRYVCVMRKNHPLADKELTLDAYCAANHLLVSFSGRAHG
                     LVDEALAQLGRERRILLTVNQFFTAGRVVATSDLITVLPRHLVASTGITGALATKELP
                     FDLPNVHIDMLWHERDTRSPAHKWLRAHLSGTTRETFAATGGRA"
     gene            10915..11403
                     /locus_tag="VM406_01435"
     CDS             10915..11403
                     /locus_tag="VM406_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family B"
                     /protein_id="HVL74651.1"
                     /translation="MSAPAIRAAVADDAPVLCAIYNHYVLTSAISFEEAPVSIDEMAA
                     RIRATSPWFVLEQDGVVAGYAYATPWRTRAAYRYSVESTVYVAPDRHGQGFGAQLYRA
                     LIAALRERGVHSVIGGIAQPNPASVALHEKLGFEQVALFREVGRKFDRWIDVGYWQLR
                     LE"
BASE COUNT         1957 a         3752 c         3908 g         1909 t
ORIGIN      
        1 gtacagccag gcgctggcaa gggccagcag caatgccagc ccgacataca gaacgaacac
       61 gcgcggccgc accagcgccc agaccgccat cgccgccgga atgctggtga cgccgccggc
      121 caccatgaag gccaggccga ccgccgggtt catccccatt ccgatcaagc cgcccacgag
      181 aggaatcgcc gccaggccgt tcaggtaggc cggaatgccg aggagtaccg cgagcggaat
      241 cgccatgccc ccgctgtcgc caagcgtacg ggcgacggct tcggccggca gccacgccag
      301 catcaggctc tcaagcacga aggccagcgc gagccagcgg ccgagcgtcc acgcgttgcg
      361 ggtcgccgat agcagaaaaa cctgggcgcg gtctgcttcc gtccagaacc gccattgcac
      421 gggcgcagcg cgcatcgcgt ccaagcgtgc gagccggcgc gtatccagtt cggcatcagt
      481 ggctcgcaag ggttgctcga gcagcgcgga acgtgtcagc gcacgggtga tccagccgcc
      541 cgccaccccc atgaagatcg ctgccgccgt cttggctatt gcaaattcca cgccgattgc
      601 gccggaagtg agtacaaaca tcacaggatc catcaccggc gaagccagcc agaaggccat
      661 gaccggcgcg agcggcaccc cggcggcgag cagaccggcg atcagcggga cgacgccgca
      721 ggagcacagt ggggagaggg cgccggcgat ggcggcgaca aggatcgcac ggccttggcg
      781 cgcggcgaag gcccggctga ccagcatctc ggcccccgct gccttgatcc cggcggccaa
      841 ggccaccgac agcgccagga tcggtgcgat ggcgccaagc gcgcgaccca ggaaaagcag
      901 gctttcctgc gcctggcgcg gatcgagcag tacgagcacg aacaggatcg cagcgatggc
      961 gagcaggacg cggtcggtgc ggcgccagag atcggcaaca ggtgatgcaa aagaggatgg
     1021 agtcatagcg cgcatggtct tctcctgtct atatatctag aaatatgggt ttgttgaggt
     1081 aaaaaaaatg gcgcatcgcc accgatcgct caaagctcct gtacgacggc cgcgcccttg
     1141 cagcattcct ccatcacgaa gtcgatcgcg ccgcgcagcc gcgggtaatt cgccctgcag
     1201 cgcacttccc gtccgcgtcg ctcctgcagc accagacccg cctcgaccag gccgcgcagg
     1261 tggaatccga gcgtcgaagc cgggattccg atcgcgttgc ggatctcgct gatgctcagc
     1321 ccctcgtcgc cggcccgcac cagcaggcgg aagatctcca ggcggacggt gttgccgagt
     1381 tcggacaggc agcgggcgac ttcatcgatg ttcatggcct gcactatagc cgaggctcgt
     1441 ccaaattaca actataaaac tagcgatata gatttatttc cgccccgccg ggcaggcggg
     1501 gctgcgtcgg cctcagtggg ggacgatttc cccgttccag cgctcccccg cgcggaactg
     1561 caggttgcgg cgcgcgctgc gctgctgttc cgcgctggcc gtggcgggcg ctgccgctgc
     1621 gccggaaggc ggcggtgaag ctgggggggc aacggtcggc gcctgggcca ctggctcgga
     1681 ggaacggctg gtgccttcag tgccggactg gccgctgctg cgttcgccga cgccatccgc
     1741 ggaagccgac gcggcgcgat ccggagcggc ggccaccggc ggtggctcga caggcgcgtc
     1801 cgttgcctcc gctcgctgtt ccggcgcgct tggtggcgcg ctggcacggc gctcgacctc
     1861 gtggtggccg tcgggagtga gaatcaccat tgtttcccca ttgctgctgg tgatatagcc
     1921 gtgtccgtct ggcagcaggt cgatgccctg tacgacgtgt gccccctcgt cgagcggcgc
     1981 acgcaccatc aagggcggtg gccggttcgc ctgaggaaag cgcgccttcg catcgatcag
     2041 cttcgcgcgc agcgtgtcgc ggaagaagtc gccgaccagc agcaccgcat tgcgtccgcc
     2101 ctgaccccaa tagtcgctgc gcatggtcac gcgctggtcg ttgaagccga cccaggcgcc
     2161 ggcgaccagt tccggatgca tcaggatgaa ccagccgtcg gtgttgtact gggtggtgcc
     2221 ggtcttgccg gcgatgtcgg cggtgatgtt gaagcggttg cgcacctcgg tgccggtgcc
     2281 ttgccgaacc acgccgcgca tcatgtcgat gagttccagc gcggtctctt cggacatcac
     2341 gtattccggc gtgcggctga attcagcgat caccttgcct tcacggtcgg tgatgcgctt
     2401 gatcgtcagc ggggcgtgat actcgccggt ctgggcgatc gtcgcgtagg aggacaccat
     2461 ttccagcagc gtgaccgggc tggtgccgag agacagcgag ggcaccaggc gcagccggct
     2521 gctgcggatg ccgacggcct tcgccaggcg cgcgacctgc tcgacgccca cgtccagcat
     2581 cacctgggcg gtgatggtgt tcttcgaaaa gaccaggccc tcgcgcatcg acatcaggcg
     2641 attggaaaag ccggacatgt cggtgggccg ccacacgccg ccatccgaga ggcggatctc
     2701 gatcttgccg tcggggtact gcagctcggg ccgcaggccg tgctccagcg ccgcgccgta
     2761 gacgaagggc ttgaacgtgg agcccggctg gcgcaccgcc tgcgcgacat ggtcgaactg
     2821 atcgatttcg aaattgcggc tgccgatcca ggccttcacc tcgcccgagc gcgggtcgat
     2881 cgccacgaaa ccggcttcaa ggcgcgtctt gaattcgcgc aggcgtgcga tgaagcgttc
     2941 gtcgccgagc agacgctgca gcgccgcttc cgccgactcg ccgctgttct gcgcgcgccg
     3001 gaactcgcgg ctctcgcgca cgaaggcttc cagcagtccc ggacgttctt cccagaagcg
     3061 gctgaacggc tcgattttct tgcgcagctt cgtatagtgc gccggcgatt gcgaggccac
     3121 ccgggcatcg cgctgcgccc attcgacgtc cgcgatgtcc tgcaggagtt tcgtctggcg
     3181 ctcgacggcg gcggcagccg cctgctgaaa gcgatcgtct atcgtgctgt gcacgatcag
     3241 gccgtcggtg tagatgttgt agtcgttggc ctcggcccat tcgatcagct ggcgccgcac
     3301 atgggcggcg aagtgcggcg cgctgcccag cggatcgggc tggcgcgtga gctgcacgtt
     3361 cagcggctcg ttgcgcaggc gctgcagcac ggccgcgtcg agcatcccgt gccgcaccat
     3421 ctgctggagc acgatgttgc ggcgccgctg cgcacgctcc gggtgcagca ccgggttgta
     3481 atagctggtg cccttgagca tgcccacgag cgtggcgctc tcgagcaggt cgagatcggc
     3541 agccgacttg tcgaaatagg tgcgcgcggc catctcgatg ccggtgacct gatacaggaa
     3601 gggcaccgaa ttcaggtagg tctcgagaat ttcgtccttg gtgtaggtct gctcgatctt
     3661 gagcgctgtg agcatctcgc gcagcttgcg ctccagcgtg cgctcgcggc ccacgcgcac
     3721 cggaaacatg ttgcgcgcaa gctgctgagt gagcgtggac ccgccctgca tgttgccgcg
     3781 cagcgtgtac cacagcgccc ccagcgtgcg ttcatagtcg atgcccttgt gctcgtagaa
     3841 gcgatgatcc tcgaccgcga tcagcgcctg caccacgtag ggcgacacct cgtcgagcga
     3901 cacgcgggcg aaatggccgc ggctgaaggt cgccaggtgc gtgcccgacg ccgacagcag
     3961 gatgctgggc tcggcgccgc gcgcctgcag catcgcatcg acgcctggaa gggtcttgtt
     4021 caatccgtgc agatagttgg ccacgaacac acccgcgcca accagcagca cgacgaatac
     4081 gccgacgctc gccagcgcca cgcgcagcac agtcctgcgc caggatcgct tcgcgccctt
     4141 cccgtcgttc tttttccgtt gccgcgccgg ttcgtctttc gtttccgcgc gtgcgtcctc
     4201 gctctccacc acgccttccc ctgctgtccg aattggcttt tggacaagcc ccttttcgaa
     4261 tggttttggt ccggacttgg aagaatcaca aggaaatcgc ccctcaatgt aagcaattgt
     4321 gcgcagtatg atgtgcagca gaagggattg cttatgcact tgagtttctg ggaacacgac
     4381 gccgcgccgg acgccgattt catcgtcgtt ggcgcggggc tgatcggcct gcagaccgcg
     4441 ctggagctgc gcgcgctcgc gccggacgcg cgcatcgcgg tgcttgaacg cggcgtgttg
     4501 ccgtacggcg cgtcgacccg caatgccggc ttcgcctgct tcggcagcct gacggaaatc
     4561 ctgcacgact tcgacacgat gggcgaagac gcggcgctgg ccgtcgtcga gcagcgctgg
     4621 cgcggcctgg cgcggctgcg cgcgcgggtg ggcgaagcgg cactcggttt cgagccgcac
     4681 ggcggctacg aactgctgag caccgcgcaa ctgcccgcgc tggaacgcat ggatgaagcg
     4741 aatgcacgcc tgcgcccgct gttcggccgc gacgtgtttc agctcgatgc ggcgatgcgc
     4801 gccgccagtg gtttcgggcc gcaggttgcc gcgctggtgg ccaatccgct cgaagggcag
     4861 ttgcactccg gacgcatgat gcgggcgctg tcccggctcg ccgtcgacgc cggcatcgct
     4921 ctgtacggcg gcgtgaccgt gcgctcgctg catgaagggg cagccggcgt ggagctgcat
     4981 gcgggcagcg ccgccgaccc gcgtgagctg cgctttcgcg cggcgcgcgt ggcgctgtgc
     5041 accaacggcc tgcttggtga gctcgctccc gcctgcgcag tgcgccccgg ccgcggccag
     5101 gtcctcgtca ccgcgccggt tgccggcttg cgttggcgcg gcacctttca catggacgag
     5161 ggttactggt acttccgcaa catcggcgat cgcgtgctgc tgggcggcgg gcgccatctc
     5221 gatttcgccg gggaagagac gagcgcgatg ggcctgagcg cggtcatcca gcaggcgcag
     5281 gaacgcttgc tgcgcgagac catcctgccg ggcagcaagg tcgcgatcga acaccgctgg
     5341 tccggcatca tgggtttttc cgccgacaag cagccgatcg tgcgcgccgt gtcgccgcgc
     5401 gtggcgatcg gtttcggctg caacggtatg ggtgtcgcgc tcggcgccga catcgccgcg
     5461 cgcaccgccg ccctgctgca cggctgctga caggatggtg tcagcagcgc ggggctagcc
     5521 tctcgggcag cggaaagaat cgttaaggct gcgctggaca agtcccgcga tggcgcgccc
     5581 gaaatcggga tggtatgcaa ggcgaaagcc ggagcgatgg cggggctatc gcgaggattt
     5641 tcaacgcagc agaccgcccg gtttctggat gtgcgacgcg cggggacctg ttcagcgtag
     5701 ccttaatccc gctcagcggt gccgcggatt gcatgcggga gtcgcggcgg gagcgcgtat
     5761 gctgtctgtt cgcgaggaag ccatgccgaa cattctttcc tgctattacc tggatgaccc
     5821 gctcaccgac gaggagctgg cgtttgtcgc gcgcacgctg accggctact gggcgcgctt
     5881 caagaccggc gcccaggaag tgcaccaggt gcgcgtgccg ggcgtgctgc ccttgccgga
     5941 cgagaacggc gagtacgcgg atagccgcga ggagcgcgcg cagcgggtca ggcacaacct
     6001 gcgccacgcc ggcatacgcg gcgacgccgg ccgacgcgtg gtgtgggtga tgccgaagga
     6061 tgcggactgg gatgccgtct tccagtttgc gatccgcgag gaaaccggct ttgcgccttt
     6121 cgttgcccaa cgctggttcc cgaaggacgg tgaacaggtg cgcggcagcc tgcgggtgat
     6181 cgataccgac atgctgctgc gcggacttga atcataggag taccgacatg agcgtcaaac
     6241 cgattcccga aggctaccgc aacatcaccc cctacctgac gatcaggaac gccgcggccg
     6301 cgatcgattt ctacaagcag gcatttggcg cggaggaacg ctttcggatg agcgagccgg
     6361 acggccgcgt cggccatgcc gagattcaga tcggcgattc gctgctgatg ttgtccgacg
     6421 aatatccgga gatgcaggcg accggtcccc agaccatcgg tggcacgccg gtgatgatgc
     6481 acctgtatgt cgaggacgtg gacgcggtgg tcaagcgtgc ggtggacgcg ggtgcgcaac
     6541 tggtgcgacc ggtggagaac cagttctacg gcgaccgcgc cggcctgctg caggacccgt
     6601 tcggccacaa gtggtgggtt tccacgcatg tcgaggacgt gtcgcccgac gaactggagc
     6661 gccgccgtca gaccgcgcac gcatagaaag aagaaagcgc gcgacatgcc cgcaacttgt
     6721 gcggcgccat cggctctcag tgtgatcggg tctctctgag actgatcgag acaggagatg
     6781 cacgatggca agcattcagc agcaggatgg acaggatcgg cagaaaaaag gcgccgcatc
     6841 cggttacgca gccggcagcg aaggatggga cagcagcgag gatttttccg atctgcccgc
     6901 ttccagccag gcagcgccgc cgccgcaatc cgaccaggag acgcagtcgg aattcgcgct
     6961 gtcgcgcgcg tcggacgacg atgagcagga cgggcgcttc tcggttgccg aggaacagaa
     7021 cttcgaccag cagtcggacg gcgcacgcag ggtcggccag gtcccggccg acgccggggc
     7081 ggcgggcctg gcgggcgccg atgccggcga tgcaatgagc gaatcgctgc agcaggacac
     7141 gcagcttgaa ggcgaaggac aggtgccgga cagcttgaaa caggcgatcg aacgctccgt
     7201 tcccccggac ggctgatggc gcgagcgcgc tgttgctttt ttaccgcagg caagcgcatg
     7261 tcatgccata tgtcatggag ttgtcagggc ggctctctat actcttctct atcggcaaca
     7321 gtgccggtgc ggagaacatc atgggaaggt cgctctacaa cagaattcag ctcggacgcc
     7381 gccggctgtt gctgaacatg cgccgcgtcc gtgcggctgc gggttttctg gcgctgtgta
     7441 ccggcatcgt ggtgtggatg atcgccacct acgaatgagc gccgcgctgg cgggccgtca
     7501 gtccgcgcct gcaagcgctg catcccggcc ctcgctgccg ggataaagcc cgcctgcgaa
     7561 cggtcacatc atgaccgggg cgcgcagccg cttgcgcgtc gcgcgtacaa tgcgccgcgc
     7621 gccctcccac ttcccttttc gcatgatcat cgccacccac tccggcaagt tccacgcaga
     7681 cgatgtctgg gccgtcgcgg tgctcgacat cgtcttcccc ggctgcgagc tgattcgcac
     7741 gcgcgacccg gcgctgattg aacgcgccga tttcgccgtc gacgtcggcg gcgtctggga
     7801 tcccgccgca ggccgcttcg accatcacca gaaaggcttc gatgccgccc gcgcttccgg
     7861 cgtcgtctat gccagtgccg gactcgtttg gaaggcgcac ggcgcgcgct gcgtggcgcg
     7921 cattgccgag cgcgaggccg gcgtgcagct cgacgacgag cgggccagcc agatcgccta
     7981 tgcgatcgat gccgatgtcg tgcagtacct cgacatgtcc gataccggcg ccgcgcgtag
     8041 tgcgcccggc ggttacggcc tgtcggcgct gatcagcggc tacaacccga actggatgga
     8101 cgagcagcat gccggcaacg ccgcggccgc ggaagcgctg cgccttgccc gcttccggcg
     8161 cgcgatggca gtgatggagg atgtcatcgt caaccaggtg aaataccgcg tgggcagcct
     8221 gcgcgcgctc gatcaggtgc gcaatggaga actgctggaa ggcggccgcg tgctgttcct
     8281 ggcaaacgcc ggcctgccgt ggtccggggt ggtgcgcaac gaaatgccga aggtgcttct
     8341 ggtgatcggc tacagcatcg gcgacgaccg ctacatgctg agtaccgtgc cggccaatcc
     8401 ggaaaccttc gaggcgcgca tggacctgcc ggcgccgtgg gccggcctgc gcgaggccga
     8461 gctggccgcc gagaccggcg tgccggatgc ggtgttctgc cacaacaagc gtttcatcgc
     8521 cgccgcgcgc tcattcgaag gcgcgctggc gctggcacgc attgcgctgc gcgacgcgcg
     8581 atgaaccccg cgcgcgcccg aactgcctgg agaccgtcat gggaatgagc accacgtttc
     8641 tcaccgaaga gccgccacgc ggcgaagtcg atacgctgcc cggtgcgacc gtgctggagt
     8701 tcggcaccgg ctggtgcggt ttctgccgtg ccgcgcagcc gctgctggcg gaggcctttg
     8761 acagccaccc tcaggtacgg cacctgaaga tcgaggatgg acgcggccgt ccgctcgggc
     8821 gctcgttccg cgtcaagctg tggccgaccc tggttttcct gcgtgacggc caggaaatcg
     8881 cccggctggt gcggccggac agtgccgccg agattcgtga cgcgctgcaa agaatcgcgt
     8941 agcgttcagt cagcattccg cggcgaaaaa aaaccccgca aagccgaagc ttgcggggtg
     9001 gaacccttcc tgggaaggaa aggaggagac actgtagaac cgattgcctc gcctacgcac
     9061 ggtagaagtc ttcacgtacc ttcaagttcc gcaaaacggt aaatccgtgg cgctgcgagg
     9121 cgcgggctga cgcagaggca atatcggcga tgcccagcgc agcggcttcc ggctttgccg
     9181 gagtggaaac aaagcgcacc gcgcgcagca gcggcgttcc ggccaggtag aacagcggaa
     9241 atgccaccgc ccaggcgacc agccagtttt ccagccactg gctgaagctg ccgcccaacg
     9301 cgagcgccac gaccgcgccg gcgaaggcgc cggaaacgaa ggccggcagc agcgaagcga
     9361 gcgccggcga caggccggca cgggcgccga ggcgctccag cagactgcct tccaggtgac
     9421 gggtctcggc agattgcagc aaggctgcgg gatggggatg caggactgcg ctcatgttcg
     9481 ttccttggca agaataggga tcagtcgcgg gaggcgatcg cgcgcaactc ggcagcaagg
     9541 gccggctggt tgcgctcgac ctcgcgcgcc atgcgatgga gcatcatcac gccggctgcc
     9601 ttctcgcggg cggcgtgcag cgcggacgcg gtacgcgggg cgagcaccat cagggccgcg
     9661 cgcagcaggc cggcgaggaa ggggcggaag aaaatgacga gggcggtcaa gaggcccagg
     9721 cccagcagcg ggcgggcaac ggcgccggcg ttggcgagca ctgcttcggc agggacgagc
     9781 gcggaaatca gggacatggt ttactccttc agtaaggaaa ctcggaaatt tctttcgttt
     9841 gtgcattgca gtatatggac gggcgagtta taactccaat tccgtttttg gatatcaaac
     9901 ataaagattc tgaatatcat ctgccgttgc ccggcacctt cactttcccg ctgccatgcg
     9961 ctttctcacc cttgacctga acctgttgcg cgtcttcgac gccgtgatga ccgaacagaa
    10021 cctcacccgc gccgcgagcc ggctggcgat gacccagccg gccgtttcca atgcgttgcg
    10081 gcgcctgcgc gaagcgctcg gtgacgagtt gctgatccgc accgcgcatg gcgtcaagcc
    10141 cacgccccgc gccgagacct tgtggccggc ggtgcgccgc gcattgaccg aactggaaga
    10201 ggcgatcacg ccggaagaga acttcgatat cgcgcgcgcc gagaacacct tccggctggc
    10261 aatggcggat gcgaccgcgg cattgtggct gccgtcgctg gtgcgctcga tcgaacgcga
    10321 agccccgctg ctgacgatcc gcatggtgcc gctgaccacg cgcgagccgc gaccgatgct
    10381 gctgcgcggc gacatcgatc tggcagtcgg cttctttccc ggcgtggtcg cccagctggc
    10441 cggggggcag gtggcctcgg tctcgccgat acgccatgag cagctgtatt cgggccgcta
    10501 tgtctgcgtg atgcgcaaga accacccgct ggcggacaag gaactcacgc tcgacgccta
    10561 ctgcgccgcc aatcacctgc tggtgagctt ctccgggcgg gcgcacggcc tggtggacga
    10621 ggcgctggcc cagctcgggc gcgagcggcg catcctgttg acggtcaacc agttcttcac
    10681 cgccggccgg gtggtcgcca cgtccgacct gatcaccgtg ctgccgcgcc acctggtggc
    10741 atccaccggc atcaccggcg cgctggcgac gaaggagctg ccgttcgatt tgccgaacgt
    10801 gcatatcgac atgctctggc acgagcgcga tacgcgcagt ccggcgcaca aatggctgcg
    10861 cgctcatctc tccggcacca cccgcgagac cttcgccgcg accggcgggc gcgcgtgagc
    10921 gcgccggcca tccgggcggc ggtcgcggac gacgcgccgg tcctctgcgc gatctacaac
    10981 cactatgtct tgaccagcgc gatcagtttc gaggaagcgc cggtgtcaat cgacgagatg
    11041 gccgcgcgaa tacgcgcgac ctcgccctgg ttcgtgctgg agcaggacgg cgtggtggcc
    11101 ggctatgcat atgcgacgcc atggcgcacg cgggccgcgt accggtattc ggtcgagagc
    11161 acggtgtatg tcgcgcccga ccggcacggg cagggattcg gcgcacagct gtatcgggcg
    11221 ctgatcgccg cactgcggga acgcggcgtg cacagcgtga taggcggcat cgcgcagccg
    11281 aatccggcca gcgttgccct gcatgagaag ctcggcttcg agcaggtcgc cctctttcgc
    11341 gaagtggggc gcaagttcga tcgctggatc gatgtgggtt actggcaact gcggctcgaa
    11401 tagctgctgt catgaaactg tcatgcggcc gtcacgatgc cgtaacgcaa gcttcctaga
    11461 atcccgtgcg tcttcaaccc caccacagga atgacgcatg aacaagccca acgacctgcc
    11521 cgtcaa
//
LOCUS       DATLGF010000044        11381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_18101,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000044 DATLGF010000000
VERSION     DATLGF010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 11381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11381
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18101"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /gene="uraD"
                     /locus_tag="VM406_01440"
     CDS             complement(<1..807)
                     /gene="uraD"
                     /locus_tag="VM406_01440"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HVL74652.1"
                     /translation="MTLTLDTLNTCPAPDFVSALGGIYEHSPWIPERAAARRPFTTIT
                     ALKQALQAVVTAASEEEQLGLIRAHPELAGKAAIAGELTKESTGEQAKAGLNNCSPEE
                     FATLHRLNADYNAKFGFPFILAVKGPDGKGYSRAQIIETFSRRLKNGRADEMAECLRQ
                     IHRIAELRINDLLGYTPALGNQIMEWAEVIGAWSDEPNNLTCAYMTNAHRKTAVQLAA
                     WMREAGMQVQIDAIGNVVGRYLSDNPDAKTLMTGSHYDTVRNGGKYDGRLG"
     gene            complement(814..1776)
                     /gene="puuE"
                     /locus_tag="VM406_01445"
     CDS             complement(814..1776)
                     /gene="puuE"
                     /locus_tag="VM406_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HVL74653.1"
                     /translation="MTDTYQDYPRDLVGYGRNPPHAQWPGGARIAVQFVLNYEEGGEN
                     CVLHGDRASETFLSEIIGAQAFEMRHMSMESIYEYGSRAGLWRLLRMFEERRLPLTIF
                     GVSMALKRHPEATAAFVELGHEIACHGLRWISYQHVDEATERAHMAEAVQIIRELTGS
                     APLGWYTGRDSPNTRRLVVEHGGFVYDADYYGDDLPFWQPVTISENGKPLTKPQLVVP
                     YTLDANDMRFAAMQGFNSGTQFFDYLKDAFDVLYAEGDPDGLNRPKMMSIGLHCRLVG
                     RPGRAAALARFLDYIQSHDKVWITRRIDIANHWREVHPYTDPAR"
     gene            2001..3512
                     /locus_tag="VM406_01450"
     CDS             2001..3512
                     /locus_tag="VM406_01450"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVL74654.1"
                     /translation="MKALRNIWLWLAIVVLVAGGWYGYRTWAGKETGPEYRIARIERG
                     AITASVSASGTLNPVVSVQVGSQVSGQLRDVLVDYNTPVKRGQLIARIDPESFEYRVR
                     QAQADLDAAQAQVMTQQANQAAQRASLAKAEVDLAEAKRDLARKQQLVERGFLSSAEQ
                     ERALAVYHAALEQVNTAKAQGGVAAAGLRNAQAVVKQREAALAQARTELGRTEIRAPV
                     DGVVIKRSVERGQTVAASLQAPELFVIAENLSDMQVEAAIDESEIGRLREGQRATFTV
                     DAFPGRSFEGTVKQIRLAAQNVSNVVTYTVVISAANPRGELLPGMTANVRVVTASRDD
                     VLKVANGALRFRPPGAAPVAQTAGRAGADIRTQLDQLDLSAEQRGQLEGVFAEERERR
                     AAAGAEQGERPRTAARNRAETRARIMAILTPEQKKKFEAMADAAESGGQPGRIYVLDD
                     KRQPRPIDVRLGLADSSATEILSPEVREGMEVIVGTALPGAANASRPPGPRLF"
     gene            3515..4249
                     /locus_tag="VM406_01455"
     CDS             3515..4249
                     /locus_tag="VM406_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL74655.1"
                     /translation="MLIETKDLAKEYVMGTNHVRALRGVSVAIDEGEFVAIMGASGSG
                     KSTFMNLLGCLDAPTSGEYRLAGESVSAMGQDQLAVIRNRRIGFVFQQFNLLPRTTAL
                     DNVELPLLYAGLGNEERRRRATLRLQQVGLGERMDHHPSQLSGGQQQRVAIARALAND
                     PSLILADEPTGALDSRTSIEIMALLQDLNRQGMTIVIVTHEHDVAEFASRLITFRDGR
                     VISDTRNAPKDALAALAALDDARDAA"
     gene            4246..5478
                     /locus_tag="VM406_01460"
     CDS             4246..5478
                     /locus_tag="VM406_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL74656.1"
                     /translation="MSWLATLKIALNALRVNKLRSGLTMLGIIIGVAAVITMVAVGAG
                     AQARVEEQIKSLGSNLMMVMPGSTTASGVRMGAGANMSLTEDDAAAIAREIPGVHVAA
                     PSNRRTEQVVAPGSNWSTQVFGVNPDYLIARDWPLTAGRMFDEMELSGSAKVAVIGQT
                     TAQYLFGDADPIDQMIRIRNVPFTVVGILERKGQSLMGQDQDDVVMLPLATARNRLFG
                     TTQGRMRAVGTIQVKVHDGVDMKEVEDQVRELLRQRKRTQPGADDPFTVRNLTEILQT
                     QEAAARIMSILLAAVASVSLVVGGIGIMNIMLVSVTERTREIGLRMAVGARGRDILTQ
                     FLVEAVTLSLIGGVIGIVLGVTGSMLVSHFAGWTTQLSPQAIVLAVGFSAGIGIFFGF
                     YPARKAASLSPIQALRYE"
     gene            5471..5731
                     /locus_tag="VM406_01465"
     CDS             5471..5731
                     /locus_tag="VM406_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HVL74657.1"
                     /translation="MSECRAGCGACCIAPSISSPIPGMPDGKPAGVRCVQLDDANRCL
                     IFGKPGRPAVCESLQPSPDMCGANREEAIFWLQRLEHLTACG"
     gene            5766..6320
                     /locus_tag="VM406_01470"
     CDS             5766..6320
                     /locus_tag="VM406_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1439 domain-containing protein"
                     /protein_id="HVL74658.1"
                     /translation="MLSRRLAVFAIVAAPLLLTSCAHLMGPRQMEIPVARLQEALSRQ
                     FPFNNRYLELLDVRVTNPRVALLPDSNRILTSMDASIAPPFMNKSWLGNLAVSGQLRF
                     DPARNALVLAEPRVETFDVMGLDPLYANQIRRVGSLLAEQLLKDVPLYTFRPDEFRYA
                     GTTFLPTQITTRSSGLVVTFEPVR"
     gene            6421..7242
                     /locus_tag="VM406_01475"
     CDS             6421..7242
                     /locus_tag="VM406_01475"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HVL74659.1"
                     /translation="MDLTLAFKAFLLGIVEGFTEFLPISSTGHLILVGSMLDFNDDKA
                     KVFEIAIQSGALLAVCWEYRARIMETVSGLFSDAAARRFAINIFIAFLPLAVLGLLFI
                     KAIKAFLFAPVPVALAFIIGGFIILIVEARAKAEPAARVPTMDDMSPLDALKVGLLQA
                     VSLIPGTSRSGASIIGGMWIGMSRKAATEFSFFLAIPTLFGATAYSMYKYRDLFSADD
                     IPWFGTGAVAAFVSAFLCVRWLLHFISRHDFRVFGWYRIVVGLLILGAAWFGWTH"
     gene            7249..9072
                     /gene="recQ"
                     /locus_tag="VM406_01480"
     CDS             7249..9072
                     /gene="recQ"
                     /locus_tag="VM406_01480"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HVL74660.1"
                     /translation="MAATLDERALQVLQSVFGYPAFRGQQADIIRQVAGGGDALVLMP
                     TGGGKSLCYQIPSLLRDGVGVVVSPLIALMQDQVDALAEVGVRAAYLNSTQDFRETVR
                     VEQAVRDGELDLLYVAPERLLTSRCMELLDDAKLALFAIDEAHCVSQWGHDFRPEYIR
                     LSILHERFPHIPRIALTATADPQTRAEIVRRLQLEEAAQFVSSFDRPNIRYQIVEKTS
                     GRNQVLDFIRAEHEGEAGIVYCLSRKKVEETAQFLCDHGIDALPYHAGMELRDRTANQ
                     ARFLREDGIVMVATIAFGMGIDKPDVRFVAHLDLPKSMEGYYQETGRAGRDGLPAEAW
                     MAYGLQDVVQQRRMIDESEADENFKRVQSAKLDAMLGLCETLSCRRVRLLEYFGQSSQ
                     PCGNCDNCLNPPQSFDGTVAVQKLLSAIYRVDQRFGAGHVLDVLRGVESEKVKQWRHE
                     ALSVFGVGSDVGETEWRAILRQAIALGLIEVDHEAYGALKLTEHARPVLRGERTVQLR
                     QYRKPAKARREAPKRKTAYAEAGLSPVEQAIFEKLRWWRMETAREHNVPAYVIFHDAT
                     MREIARAKPSSLNDLRGISGVGEKKLETYGEEIVSLIAGME"
     gene            complement(9226..9945)
                     /gene="trmB"
                     /locus_tag="VM406_01485"
     CDS             complement(9226..9945)
                     /gene="trmB"
                     /locus_tag="VM406_01485"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HVL74661.1"
                     /translation="MSDTDKPDTPARNMLYDPTERRIRSFVTRAGRLSTAQAKALETL
                     GPRFCISYQKAAFDFDAAFGRPAPTILEIGFGMGDTTAAIAAAMPEKNFIGVEVHTPG
                     VGSLLKLIGEQNLSNLRIIQHDAFEVVTNMIKPASLAGVHIFFPDPWHKARHNKRRLI
                     QAPFVELLSSRIAPGGYIHCATDWQDYAEQMLQVLAEEPTLANTADGYAPRPDYRPVT
                     KFENRGLRLGHGVWDLVFVKR"
     gene            10706..>11381
                     /locus_tag="VM406_01490"
     CDS             10706..>11381
                     /locus_tag="VM406_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74662.1"
                     /translation="MHDSPPVSKVYYRPIEAAIRWAGLLKRKKDILCLISSPRHFPTA
                     LNFPGWAELRLCTERIYDAIFNGELPYGCNGITQNDKALWDSPDLSIRHLDLKRWMRD
                     HYPEERPAFLFSRHERIAHPVITLETGQAMLVERQALKAELAQCRRQFQTLQEQHHVL
                     SKQAGVISACNEGSLTDRAESTYQHIIGGMLDLMLGQSPAGSPYSCFRTQEAIVSALI
                     AHHDGIM"
BASE COUNT         1957 a         3687 c         3711 g         2026 t
ORIGIN      
        1 gcccaggcgc ccgtcgtact tgccgccgtt gcgcacggtg tcgtagtgcg agccggtcat
       61 cagcgtcttc gcatccgggt tgtcggacag gtagcggccg accacgttgc cgatcgcatc
      121 gatctgcacc tgcatgccgg cctcgcgcat ccaggccgcc agctgcaccg cggtcttgcg
      181 gtgcgcattg gtcatgtacg cacaggtcag gttgttcggc tcgtcgctcc aggcgccgat
      241 cacctccgcc cattccatga tctggttgcc cagcgccggc gtgtagccga gcaggtcgtt
      301 gatgcgcagt tcggcgatgc ggtggatctg gcgcaggcat tccgccattt cgtcggcgcg
      361 gccgttcttc agccgccggc tgaaagtctc gatgatctgc gcgcgactgt agcccttgcc
      421 gtcgggcccc ttgaccgcga ggatgaacgg aaagccgaac ttcgcgttgt agtcggcgtt
      481 gagccggtgc agcgtggcga actcttccgg gctgcagttg ttgagtccgg ccttggcctg
      541 ctcgccggtc gattccttcg tcagttctcc ggcgatcgcc gccttgccgg cgagctccgg
      601 gtgggcgcgg atcaggccga gctgttcctc ctcgcttgcc gcggtgacca ccgcctgcag
      661 cgcctgtttg agcgcggtga tggtggtgaa cggacggcgc gcggccgcgc gttcgggaat
      721 ccacggcgaa tgctcgtaga tgccgcccag cgcgctgacg aagtcggggg cgggacaggt
      781 gttgagtgtg tcgagcgtga gggtcatggg cggtcagcga gcgggatcgg tgtaagggtg
      841 gacttcgcgc cagtgattgg cgatgtcgat gcggcgcgtg atccagacct tgtcgtgcga
      901 ctggatgtag tcgaggaagc gcgccagcgc ggcggcgcgg ccggggcggc cgaccagccg
      961 gcagtgcagg ccgatcgaca tcatcttcgg ccggttcagt ccgtccgggt cgccctcggc
     1021 atacagcacg tcgaacgcat ccttcaggta gtcgaagaac tgcgtgccgg aattgaaacc
     1081 ctgcatcgcc gcaaagcgca tgtcgttcgc atccagcgtg tagggcacga ccagttgcgg
     1141 cttcgtcagc ggcttgccgt tctcgctgat cgtgaccggt tgccagaacg gcaggtcgtc
     1201 gccgtagtag tcggcgtcgt agacgaaacc gccatgctcg accacgagcc gccgggtatt
     1261 cggcgagtcg cgcccggtgt accagcccag cggcgcggaa cctgtcagct cgcggatgat
     1321 ctgaacggct tcggccatgt gcgcgcgctc ggtcgcctcg tcgacatgct ggtaactgat
     1381 ccagcgcagt ccgtggcatg caatctcgtg gcccagttcg acgaaggcgg cggtcgcttc
     1441 cggatgacgc ttgagcgcca tcgacacgcc gaagatggtc agcggcaggc ggcgttcctc
     1501 gaacatgcgc aacagccgcc acagcccggc gcgcgagccg tactcgtaga tcgactccat
     1561 gctcatgtgg cgcatctcga aggcctgggc gccgatgatc tcggacagga aggtctcgga
     1621 tgcgcggtcg ccgtgcagca cgcagttctc gccgccttcc tcgtagttga ggacaaactg
     1681 cacggcgatg cgggccccgc cgggccactg cgcatgcggg ggattgcggc cgtagccgac
     1741 caggtcgcgc gggtaatctt gataagtgtc agtcacggga agtcagcttc agcggatgtc
     1801 ggatgatggt ataggcaagc ggcaggccag tcatatgcga atcgagatag ccggcctagt
     1861 gtcaagaata tactttcgcg ccatgccgcg ccgggccgtt cctccggcag gggacacagt
     1921 acgatacaaa gcgtgaccca cccccgcacc cccggccgtt acaattcccg ggcaccttct
     1981 tccaactttt tcccttatta atgaaagctt tacgaaacat ctggctttgg ctggcgatcg
     2041 tcgtgctggt tgcgggcggc tggtacggct accgcacctg ggccggcaag gaaaccggcc
     2101 ccgagtaccg catcgcgcgc atcgagcgcg gcgcgatcac cgccagcgtg tcggcttccg
     2161 gcacgctgaa tccggtggtg tcggtgcagg tgggctccca ggtgtccgga cagttgcgcg
     2221 acgtgctggt cgactacaac acgccggtca agcgcggcca gctgatcgcg cgcatcgatc
     2281 cggagtcctt cgagtaccgt gtgcgccagg cgcaggcgga cctcgacgcc gcccaggccc
     2341 aggtgatgac ccagcaggcg aaccaggccg cgcaacgcgc atcgctcgcc aaggccgagg
     2401 tggatctggc cgaagccaag cgcgatctgg cgcgcaagca gcagctggtg gaacgcggct
     2461 ttttgtccag cgccgaacag gaaagggcgc tggccgtcta ccacgccgca ctcgagcagg
     2521 tgaataccgc gaaggcccag ggcggcgtgg ccgcggccgg cctgcgcaat gcgcaggcgg
     2581 tggtcaagca gcgcgaggcg gcgcttgcgc aggccaggac cgaactggga cgcaccgaga
     2641 tccgcgcgcc ggtcgacggt gtggtgatca agcgcagcgt cgagcgcggc cagaccgtgg
     2701 cggccagcct gcaggcgccg gaactgttcg tgattgcgga aaacctgtct gacatgcagg
     2761 tcgaggcggc gatcgacgaa tccgagatcg gtcgcctgcg cgaggggcag cgcgcgacct
     2821 tcaccgtcga tgccttcccg ggacgcagct tcgaaggcac ggtcaagcag atccggctcg
     2881 ccgcgcagaa cgtgtcgaac gtggtcacgt atacggtagt catcagcgcg gccaatccgc
     2941 gcggcgagct gctgcccggc atgactgcga acgtgcgtgt ggtgacggcc agccgcgacg
     3001 atgtgctgaa ggtcgccaac ggcgcgctgc gctttcgccc gccgggcgcg gcaccggtcg
     3061 cgcagacggc cggccgcgcc ggcgccgaca tccgcacgca gctcgaccag ctggacctga
     3121 gcgccgagca gcgcggtcag ctcgagggcg tcttcgccga ggaacgcgag cgccgcgccg
     3181 cggccggagc ggagcagggc gagcgcccgc gcaccgcagc gcgcaaccgt gccgaaacgc
     3241 gcgcgcgcat catggcgatc ctcacgcccg agcagaagaa aaagttcgag gcgatggcgg
     3301 acgcagccga atccggcggc cagccgggac gcatctatgt gcttgacgac aagcgccaac
     3361 cgcgtccgat cgacgtgcgc ctgggcctgg ccgacagcag cgcgaccgag atcctttcgc
     3421 ccgaggtgag agagggcatg gaagtgatcg tcggcaccgc gctgccgggc gccgccaatg
     3481 catcccgccc gccgggcccg cgcctgttct gacgatgctg atcgaaacca aagacctggc
     3541 gaaggaatac gtgatgggca cgaaccatgt tcgtgcgctg cgcggggtgt cggtcgcgat
     3601 cgatgaaggc gaattcgtcg cgatcatggg cgcgtccggt tcgggcaagt ccacgttcat
     3661 gaacctgctc ggttgcctcg acgcgccgac ttccggcgaa taccggctgg ccggcgagag
     3721 cgtctcggcg atggggcagg accagctggc cgtgatccgc aaccgccgca tcggcttcgt
     3781 gttccagcag ttcaacctgc tgccgcgcac gaccgcgctg gacaacgtcg aactgccgct
     3841 cctgtacgcc ggactcggca acgaggaacg ccgccggcgc gcgacgctgc ggctgcagca
     3901 ggtcggcctg ggcgagcgca tggaccacca tccgtcgcag ctctccggcg gccagcagca
     3961 gcgcgtggcg attgcgcggg cgttggccaa cgatccgtcg ctgatcctcg ccgacgaacc
     4021 gaccggcgcg ctcgattcgc gcaccagcat cgagatcatg gcgctgctgc aggatctgaa
     4081 ccggcagggc atgaccatcg tgatcgtcac ccatgagcac gacgtggccg aattcgcatc
     4141 gcggctgatt accttccgcg acggccgcgt catttccgac acccgcaacg cgccgaagga
     4201 tgcgctcgcc gcgctggccg cgctcgatga cgcgagggac gccgcatgag ctggctggca
     4261 acgctgaaga tcgcgctcaa tgcgctgcgg gtcaacaagc tgcgctccgg gctcacaatg
     4321 ctcggcatta tcatcggcgt ggccgcggtg atcacgatgg tcgccgtcgg cgccggcgcg
     4381 caggcgcgcg tcgaggagca gatcaagagc ctggggtcga acctgatgat ggtgatgccc
     4441 ggctccacca ccgcctccgg cgtgcgcatg ggcgcgggcg cgaacatgag cctgaccgaa
     4501 gacgatgcgg ccgcgatcgc acgcgagatc cccggcgtgc atgtggccgc gccgtcgaac
     4561 cgccgcaccg agcaggtggt cgcgcccggt tccaactggt cgacccaggt gttcggcgtc
     4621 aatccggatt atttgatcgc acgcgactgg ccgctcaccg ccggacgcat gttcgacgag
     4681 atggagctgt ccggctcggc caaggtagcg gtgatcggcc agaccaccgc gcagtacctg
     4741 tttggcgacg ccgacccgat cgaccagatg atccgcatcc gcaacgtgcc gttcaccgtc
     4801 gtcggcatcc ttgagcgcaa gggccagagc ctgatggggc aggaccagga cgacgtcgtg
     4861 atgctgccgc tggccaccgc gcgcaaccga ctgttcggca ccacgcaggg ccgcatgcgc
     4921 gcggtcggca cgatccaggt gaaggtgcac gacggcgtgg acatgaagga ggtcgaggac
     4981 caggtgcgcg aactgctgcg gcagcgcaag cgcacccagc cgggcgccga cgacccgttc
     5041 accgtgcgca atctgaccga aattctgcag acgcaggaag cggccgcgcg catcatgagc
     5101 atcctgctgg ccgcggtggc gtcggtgtcg cttgtcgtcg gcggcatcgg catcatgaac
     5161 atcatgctgg tgtcggtgac cgagcgcacc cgcgagatcg gtctgcgcat ggcggtcggc
     5221 gcgcgcgggc gcgacatcct cacgcaattc ctggtcgagg cggtcacgct gtcgctgatc
     5281 ggcggcgtga tcggaatcgt gcttggcgtg acgggctcga tgctggtcag ccatttcgcc
     5341 ggctggacca cgcaactgtc gccgcaggcc atcgtgctgg ccgtcggctt ttcggccggg
     5401 atcggcatct tcttcggctt ctacccggcg cgcaaggcgg catcgctgtc gccgatacag
     5461 gcgctgcgct atgagtgagt gccgcgccgg ctgcggcgcc tgctgcatcg cgccgtcgat
     5521 ctcgtcgccg attccgggca tgccggacgg caagcccgcc ggcgtgcgct gcgtgcagct
     5581 cgatgacgcg aatcgctgcc tgatctttgg caagcccggc cgcccggcgg tatgcgaaag
     5641 tctgcaaccg tcgcccgata tgtgcggcgc aaaccgggaa gaggcgatct tctggctgca
     5701 gcgcctggaa cacctgaccg cttgcggctg actacaatat gccgtccact ctcaccggag
     5761 gaaacatgct ttcacgccga cttgccgtct ttgcaatcgt tgccgcgccg ctgctgctga
     5821 cttcctgtgc gcacctgatg ggcccgcgcc agatggagat cccggttgcg cgcctgcagg
     5881 aagcgctgtc gcgccagttc ccgttcaaca accgctacct cgaactgctc gacgtgcgcg
     5941 tgaccaatcc ccgcgtcgcg cttttgccgg acagcaatcg catcctgacc tcgatggacg
     6001 cgtcgatcgc accgccgttc atgaataagt cgtggctggg caatctcgcc gtttccggcc
     6061 agctgcgctt cgatccggcg cgcaatgcgc tggtgctggc agagccgcgc gtggagacct
     6121 tcgacgtgat gggactcgat ccgctgtacg cgaaccagat ccgtcgcgtg ggcagcctgc
     6181 tggccgagca gctgctgaag gatgtgccct tgtacacatt ccggccggat gaattccgct
     6241 atgcgggaac gacgttttta ccgacgcaga tcacgacgcg ctcgagtgga ctcgtggtaa
     6301 ccttcgagcc ggttcgctga ggcgggatcc ttcggccgct cgcgccgcga aagcgtctcg
     6361 gtaccgtatc aatccgatcg cgccgcggcc gtcgccgtcc gcatcaccac ccgagcacac
     6421 atggatctca ccctcgcatt caaggccttc cttctcggca tcgtcgaagg cttcaccgaa
     6481 ttcctgccga tctcgtcgac cggccacctg atcctggtgg gcagcatgct cgacttcaat
     6541 gacgacaagg ccaaggtgtt cgagatcgcg atccagtccg gggccctgct ggcggtgtgc
     6601 tgggagtacc gggcgcgcat catggaaacc gtctcaggtc tcttcagtga cgcggcggcc
     6661 cggcgcttcg cgatcaatat tttcatcgcc tttctgccgc tggccgtgct cggcctcttg
     6721 ttcatcaagg caatcaaggc cttcctgttc gctccggttc cggtggcact ggccttcatc
     6781 atcggcggct tcatcatcct gatcgtcgaa gcgcgagcca aggccgaacc cgctgcccgc
     6841 gtgccgacga tggacgacat gtcgccgctg gatgcgctga aggtcggcct gctgcaagcc
     6901 gtctcgctga tcccgggcac cagccgctcc ggcgcttcga tcattggcgg catgtggata
     6961 gggatgtcgc gcaaggcggc caccgagttc tcgttcttcc tggcgattcc gacgctgttc
     7021 ggcgccaccg cgtactcgat gtacaagtac cgcgacctgt tttccgccga cgacattccc
     7081 tggttcggca ccggcgcggt ggcggccttc gtctctgcct tcctgtgcgt gcgctggctg
     7141 ctgcatttca tttcgcgcca cgatttccgg gtgttcggct ggtaccgcat cgtggtcggc
     7201 ctgctgatcc tgggcgccgc ctggttcggc tggacacatt gataggctat ggccgctacg
     7261 cttgacgaac gcgcgctgca ggtcctgcag tcggttttcg gttatcccgc gtttcgcggc
     7321 cagcaggccg acatcatccg ccaggtcgcc ggtggcggcg acgccctggt gttgatgccc
     7381 accggcggcg gcaagtcgct gtgctatcag attccatccc tgctgcgcga cggcgtcggc
     7441 gtggtcgtct ccccgctgat cgcgctgatg caggaccagg tcgacgcgct cgccgaggtc
     7501 ggcgtacgcg ccgcctacct gaattccacc caggatttcc gcgagaccgt gcgcgtcgag
     7561 caggctgtgc gcgacggcga gctcgacctg ctctacgtcg ctccggaacg cctgctcacc
     7621 tcgcgctgca tggaactgct cgacgacgcg aagctcgcgc tgttcgcgat cgacgaggcg
     7681 cactgcgtgt cgcaatgggg ccacgacttc cggcccgagt acatcaggct gtcgatcctg
     7741 cacgagcgct tcccgcacat cccgcgcatc gcgctgaccg cgaccgccga tccgcagacg
     7801 cgcgccgaaa tcgtgcgccg cctgcagctg gaggaggccg cgcagttcgt gtcctcgttc
     7861 gaccggccga acatccgcta ccagatcgtc gagaagacca gcggccgcaa ccaggtgctc
     7921 gacttcatcc gtgccgagca tgaaggcgag gccggcatcg tgtactgcct gtcacgcaag
     7981 aaggtcgagg aaacggcgca gttcctgtgc gaccacggca tcgacgcgct gccttaccac
     8041 gccggcatgg aactgcgcga tcgcacggcc aaccaggcgc gcttcctgcg cgaggacggc
     8101 atcgtgatgg tcgccaccat cgccttcggc atgggcatcg acaagccgga tgtacgcttc
     8161 gtcgcccatc ttgatctgcc caagagcatg gaaggctatt accaggagac cggccgcgcc
     8221 ggtcgcgacg gcctgcccgc cgaagcatgg atggcctacg gcctgcagga cgtggtgcag
     8281 cagcgccgca tgatcgacga atccgaagcc gacgaaaact tcaagcgcgt gcagtccgcc
     8341 aagctcgacg caatgcttgg cttgtgcgaa accctgtcct gccggcgcgt gcgcctgctc
     8401 gaatatttcg gccagtcgtc tcagccctgc ggcaattgcg acaactgcct gaatccgccg
     8461 cagtcgtttg acggcacggt cgcggtgcag aagctgctgt cggcgatcta ccgggtcgac
     8521 cagcgcttcg gcgccgggca tgtgctggac gtgctgcgcg gcgtggaatc ggaaaaagtg
     8581 aagcagtggc gccacgaggc gctgtccgtg tttggcgtgg gcagcgatgt cggcgagacg
     8641 gaatggcgcg cgatcctgcg ccaggcgatt gcgctcggtc tgatcgaagt cgatcacgag
     8701 gcctacggcg cgctcaagct caccgaacac gcgcgcccgg tattgcgcgg cgaaaggacg
     8761 gtgcagttgc ggcagtaccg caagccggcg aaagcgcgcc gtgaagcgcc gaagcgcaag
     8821 accgcttacg cggaagcggg gctttctccc gtcgagcagg cgatcttcga gaagctgcgc
     8881 tggtggcgca tggaaaccgc gcgcgaacac aatgtgccgg cctacgtgat cttccacgac
     8941 gcgacgatgc gcgagattgc ccgcgccaag ccgtcttcac tcaatgacct gcgcgggata
     9001 tccggcgtcg gcgagaagaa gctcgagacc tacggcgagg agatcgtgag cctgattgcc
     9061 gggatggagt agtaggagcg gggttcggcg ccgtccgggg tgcgggcgga tgaggttctt
     9121 cgcgttcgct cggaacgaca ggaaatattt gtcattccga acgcagtgag gaatctccag
     9181 cgttcaagcc gcctcagccc tcagagcggg cggtgtacga agccgctacc gcttcacgaa
     9241 caccagatcc cacaccccgt gcccgagccg caggccgcgg ttttcgaact tggtcaccgg
     9301 ccgatagtcc ggccgcggcg catagccgtc ggcggtattg gccagcgtcg gctcctctgc
     9361 gagcacctgc agcatctgct ccgcgtagtc ctgccagtcg gtcgcgcaat gaatataccc
     9421 gcccggcgcg atccggctcg acagcagttc gacgaatggc gcctggatca ggcggcgctt
     9481 gttgtgccgt gccttgtgcc agggatcggg aaagaagata tgcacaccgg ccagcgatgc
     9541 cggcttgatc atattcgtga cgacctcgaa cgcgtcgtgc tggatgatgc gcaggttgga
     9601 gaggttctgc tcgccgatca gcttcagcag gctgcccacg cccggcgtgt gcacttcgac
     9661 gccgatgaag ttcttctccg gcatcgctgc cgcgatcgcc gcagtcgtgt cgcccatgcc
     9721 gaaaccgatc tccaggatgg tcggggcggg gcgtccgaat gccgcgtcga agtcgaacgc
     9781 cgccttctgg taagagatgc aaaagcgcgg gccgagggtc tcgagcgcct tggcctgggc
     9841 ggtcgacagg cggccggccc gcgtgacgaa gctgcggatg cggcgctcgg tcgggtcgta
     9901 caacatgttg cgggctgggg tgtcgggctt gtcggtatcg ctcatgcttg agagtcgtgc
     9961 gggcgaaaaa aaagcacctg gcggtgcggt ggaacggcat ctgacggcgc cgggacggcg
    10021 ttggagcggg tgaaggaaac aaaacaccgc acccaaccca ttgattgaaa aaagattcct
    10081 tctgttcgct caacgggtaa agaccatatt atgtaccgcc ccttccttct tcggcaagta
    10141 aagctgtctg ctgacatctt ctgacggcca ctcactgcca ccaaggcttg gtcaccatgc
    10201 gacttccggc atcgatctct ccaagctcac cctcatgctc tgggacgcgc tgaaagcccc
    10261 gtaattcacg cgctgcttgc ttctatttca gtctgacagt atttcccaca ggtcttaagc
    10321 acgcgtacgg atcagccgaa catacccagc cttgctacat ggcggcactc atcgaacttg
    10381 tgacgagcat gctgatagac ccgacgcaga gcagatgatg caaagcgggt cttgctgaat
    10441 tcccgcctag caataacgct cttgcaaacg cgcaactggc atgcgtcgca gtgctgttgt
    10501 gagattccgc cagcatcacc gatgcagtca agcgtggaac ggctcaaaca tccaactttt
    10561 tagatagaca gcccgcgacg atcaggccga ggcaacactc ggcgcgcatg aatgagcgtt
    10621 gctcattctc tgctgttggc tacgcccgag tactcaggtc ttgatgcgct attgacgtaa
    10681 atattccttg tagggagatg gatgcatgca cgacagtcca cctgtgtcta aggtctatta
    10741 tcgaccaatc gaagcagcca tacgctgggc aggtttgctg aagcgcaaga aagatatctt
    10801 gtgcttgatc tcgtcaccga ggcactttcc cacggcgctc aacttccctg gatgggccga
    10861 gctacgcctg tgcaccgagc gcatttacga cgcgatcttc aacggcgaac ttccctatgg
    10921 ctgcaatggc atcacacaga acgacaaggc gctctgggat tcccccgatc tctccatcag
    10981 acatctggat ctcaagcgtt ggatgcggga tcactaccct gaagagcgac cagcctttct
    11041 cttcagccgc cacgagcgca ttgcgcatcc cgtcatcacc ttggaaacgg ggcaagcgat
    11101 gctggtggag cggcaggcat tgaaggcgga actggcgcaa tgccgtcgcc agttccagac
    11161 gctgcaggag cagcatcacg tcctgtcgaa acaggccgga gtgatttccg catgcaacga
    11221 gggctcgctc acggatcgcg cggagtccac ctaccagcac atcatcggcg gcatgcttga
    11281 tctgatgctg ggccagtctc cggcaggcag tccttactcg tgcttcagga cacaggaagc
    11341 catcgtcagc gccctgattg cgcatcacga cggcatcatg g
//
LOCUS       DATLGF010000045         7966 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_206892, whole genome shotgun sequence.
ACCESSION   DATLGF010000045 DATLGF010000000
VERSION     DATLGF010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 7966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7966
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206892"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VM406_01495"
     CDS             <1..428
                     /locus_tag="VM406_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HVL74663.1"
                     /translation="PAPAASVAPASPPAAPAARAPAAVSTPSVAGAPPVGSESPARSD
                     DGARALALLEGRADPRTTEKKAEKFVIQVAALASQEKVNELQGRLAGAGIKSFTQKVA
                     TESGERTRVRVGPFASRDEAEKMRERIVKMGLNGTLVPA"
     gene            430..918
                     /locus_tag="VM406_01500"
     CDS             430..918
                     /locus_tag="VM406_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HVL74664.1"
                     /translation="MTLFDYLVLVILACSIIISTLRGLVREILSLLSWIVSFVVANAL
                     SEDFAQWLPEIFAGKMTRLIVAFLVLFVGTRLLMGLVSMAVDALVKAAGLTLADRGLG
                     GLFGLARGLVLVMAGVLLCGMTAIPQQPFWTQALFSPLAETAARTVKPYLPGDLARHV
                     SF"
     gene            944..2461
                     /gene="purF"
                     /locus_tag="VM406_01505"
     CDS             944..2461
                     /gene="purF"
                     /locus_tag="VM406_01505"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HVL74665.1"
                     /translation="MCGIVGVVSQGPVNQLIYDALLLLQHRGQDAAGIATNHGNTFSM
                     HKANGLVRDVFRTRNMRSLPGTAGIGQVRYPTAGSSSEEEAQPFYVNAPFGIILAHNG
                     NLTNWEQLKNELFRNDRRHINTDSDSEVLLNVLAHEIQQATSGYSLDPSALFKAVSAL
                     HRRVRGSYAVVAQIAGYGLLAFRDPFGIRPLCIGFNETDQGPEYMVASESVALEGLGF
                     RFLRDVMPGEAIFIDLDGHLYNQQCADNPILAPCAFEFVYLARPDSTIDGASVYASRL
                     KMGEHLAAKIARQFPAGDIDVVMPIPDSSRPAAMELALKLNIAYREGFIKNRYIGRTF
                     IMPGQALRRKSVRQKLNAIGSEFKGKSVLLVDDSIVRGTTSREIVQMARDSGAKRVIF
                     ASAAPPVMFPNVYGIDMPTRSELIAHGRSEEEVCREITADALVYQDVEALRRSISEVN
                     PLLKRFEASCFDGVYVTGDVSPDYLERIEVARNTPKPQAEEGARSQLNLGLARAD"
     gene            complement(2468..5797)
                     /locus_tag="VM406_01510"
     CDS             complement(2468..5797)
                     /locus_tag="VM406_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVL74666.1"
                     /translation="MATILIVDDHVLNRQFLMVLLGNGGHRLLEAKDGAEGLELAQRH
                     CPDLVIADILMPNMDGYEFVSRMRSIAQIAETPVIFYTAAYHEREAATMALACGVRWV
                     LPKPSEPALILETVHQALGTPADQLPEPAEPALSPSSERLDGLNRQLNAYLEEVETSR
                     HMMSRLVRDGDREGTDTGHLQQVVDRLSNALSSLQAVSLRLTALIELGIELSTERDPD
                     RLLQIGCRVVQDLTVAKYAGIGILDADGDGLRHWVFRGLDEDAASGVASMNPRLGVFG
                     TALAQRRPLRLSGLEPAARPAGLPAAHPPIRSLLVVPLVSQDKVFGWLYLADKLGAEG
                     FGEVDERAAGTVAAQLAVIYENLSLYGEIRKQHDRLALEVGDRQHAQDALRRTLRART
                     VMAECNHAMVHAGDEAQLLNGTCAALVEAGRYQAAWISYVDEQDGSQTVVASAGAVPR
                     QVRQNPGAAGPNGNLDSERSWLVIEMGAGDEAPHQELALDHGVSMSLPLRHNGKVFGM
                     LTIHEADSNAFDREHVDMLKGLARDIAFGVTSLRTKAAREKAERDLRATEDKLSSILD
                     SIDNVVWSATDCEFIYINSMVEHIYGRKADEFYADRHLWFHVIHPEDRAAARDAHARL
                     PESGTFSQEYRIVRPDGEIRWIEERARAIRNARGELLRVDGVAIDISKRKAYEARIEF
                     LADHDALTGLANRNLLKDRLTQAMAHARRSGEQVALLFLDLDRFKGVNDSFGHHLGDA
                     LLLQVAARLREVVREGDTVARQGGDEFIILLCGVRRVEDVTTVAGKIFDAFSEAFTVE
                     GRDLYVSTSIGITLFPDDADDIQSLLRNADTAMYRAKEEQGNAFHFYSREMSERALER
                     MELESALRRAIERKEFELHYQPKVDARSRRIIGAEALIRWNHPRLGLVSPTRFIPMAE
                     EIGLIVPIGDWVLKTACAQNKAWQQAGLPRICVSVNLSARQFAQEGLVGSVERTLAHI
                     DLAPQDLELELTESVLMNGAGHYENKLRELQDLGVQLSIDDFGTGYSSLSYLKRFPLH
                     HLKIDQSFVRDIANDVDDAAITSTVISLGHSLNLKVIAEGVETEEQVRFLRDHDCDEM
                     QGYYFSRPLPANEFASLLQRT"
     gene            6072..7313
                     /locus_tag="VM406_01515"
     CDS             6072..7313
                     /locus_tag="VM406_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HVL74667.1"
                     /translation="MTDHKKYGFTTTLLHSDRRKPIEHGALHKPVHTSVTFGYGDARE
                     LADVFQGKKPGYRYGRQGNPTVSALEDKVNAMEEGLTTICFSTGMAAVGALVQGLLRT
                     GDHVVSSAFLFGNTNSLWQTVDAQGMPVSFVDATDAEAVAAAITPATRLVFVETIANP
                     RTQVADLKRIGELCRARGILYVVDNTMTSPYLFRPASVGAGLVVNSLTKSIGGHGNAL
                     GGSLTDTGLYDWTRFPNIADNYKRFPSAQWGMAQIRAKALRDFGAALGPEAAHHLAVG
                     AETMALRMERSCANALALARMLEQDARVAAVYYPGLVSHPQHQLASELFRAHGYLFSF
                     ELHEGLDCFAYLNRLRLAVPASNLGDNRTLVIPVAHTIFHEMGPERRAGMGIADSLIR
                     VSVGIEDTDDLLEDFSEALAL"
     gene            7412..>7966
                     /locus_tag="VM406_01520"
     CDS             7412..>7966
                     /locus_tag="VM406_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSHA biogenesis protein MshE"
                     /protein_id="HVL74668.1"
                     /translation="MPRPEKVRLGEILVQQKLLSDEQLQFALSEQKRTGRKLGRVFID
                     NGFVTEEQICGALAKQLNIPYVNLKFFNIKPEIVRLLPETQARRFRALVLEDRHGALL
                     IGMADPTDLFAYDEISRTVRRGIELAVVNEGELLQTIDRIYRRTEEITDLARELEQDL
                     GETAVDFGGLAGTPGIEEAPVVKLL"
BASE COUNT         1382 a         2543 c         2549 g         1492 t
ORIGIN      
        1 gtcctgcccc tgcagcctcg gttgctcccg catctccccc ggctgcacct gcggcccgcg
       61 cgccggccgc cgtttcgacg ccatccgttg caggcgcgcc gccggtcggc tccgagagtc
      121 ctgcgcgcag cgacgacggc gcacgtgcac tggcgctgct ggaaggccgc gcagacccgc
      181 gcacgaccga gaaaaaggcg gaaaaatttg taatccaggt tgcggcactt gcgtcgcagg
      241 aaaaggtcaa cgaactgcaa ggcaggttgg cgggcgccgg catcaagtcg tttacgcaga
      301 aggtggccac cgaaagcggc gaacgcaccc gggtgcgagt gggaccattc gccagccggg
      361 acgaagcgga gaagatgcgc gagcgcatcg tcaagatggg tctgaacggt acgctggtgc
      421 cggcctgacg tgacgctgtt cgactatcta gtcctggtga tactggcgtg ttcgatcatc
      481 atcagcacgc tgcgcgggct ggtcagggaa attctctcgc tgctgagctg gatcgtttcc
      541 ttcgtggtcg ccaatgcgct gagcgaggat ttcgcgcaat ggctgcccga gatattcgcg
      601 ggaaagatga cgcgactgat cgtcgctttc cttgtactgt tcgtcggaac gcggctgctg
      661 atggggctcg tgagcatggc agtggatgcc ttggtgaagg ccgccggcct gacgctggcc
      721 gatcgtgggc tgggcggcct gttcggcctg gcgcgcggac tggtgctggt catggcgggg
      781 gtgctgttgt gcggcatgac ggccattccg cagcagccgt tctggaccca ggccttgttc
      841 agcccgctgg ccgaaaccgc ggcgcgcacc gtcaaaccct atctgccggg cgatctcgcg
      901 cggcacgtaa gtttttaatc tctctccatc gaggaaatcc atcatgtgcg gcattgtcgg
      961 cgttgtttcc caaggcccgg tcaatcagct gatttacgat gcgctgctgc tgctgcagca
     1021 ccgcggccag gacgctgcag gcatcgcgac caatcacggc aacacgttct cgatgcacaa
     1081 ggccaacggc ctggtgcgcg atgttttccg cacccgcaac atgcgctcgc tgcccggcac
     1141 cgcaggcatc ggccaggtgc gctatccgac cgccggttcg tcgagcgagg aggaagcgca
     1201 gccgttctac gtgaacgcgc cgttcggcat catcctcgcg cacaacggca acctgaccaa
     1261 ttgggagcag ctgaagaatg agctgtttcg caatgaccgc cggcacatca acacggattc
     1321 cgattcggag gtcctgctca acgtgctcgc gcacgaaatc cagcaggcga cttccggcta
     1381 ttcgctcgat ccatccgcgc tgttcaaggc ggtgtcggcg ctgcaccggc gcgtgcgcgg
     1441 ctcgtatgcg gtggtcgccc agatcgccgg ctacggcctg ctcgcgttcc gcgacccgtt
     1501 cggcatccgg ccgctttgca tcggcttcaa cgagaccgac cagggcccgg aatacatggt
     1561 ggcgagcgaa tcggtggcgc tggaaggcct gggtttccgc ttcctgcgcg acgtgatgcc
     1621 gggcgaggcg atcttcatcg atctggatgg ccacctgtac aaccagcagt gcgcggacaa
     1681 tccgatcctg gcgccgtgcg cattcgaatt cgtctacctg gcccgccccg attccaccat
     1741 cgacggcgcc tcggtctacg cgtcgcggct gaagatggga gagcatctgg ccgcgaagat
     1801 tgcgcgccag tttccggccg gcgacatcga cgtggtgatg ccgattccgg attcctcccg
     1861 ccccgctgcg atggagctgg cattgaagct caacatcgcg taccgcgaag gcttcatcaa
     1921 gaaccgctac atcggccgca ccttcatcat gcccggccag gcgctgcgca ggaaatcggt
     1981 gcgccagaag ctcaatgcga tcggcagcga attcaagggc aagtcggtgc tgctggtgga
     2041 tgactccatc gtgcgcggca cgaccagccg cgaaatcgtg cagatggcgc gcgactccgg
     2101 cgccaagcgc gtgatcttcg cgtcggccgc gccgcccgtg atgttcccga acgtgtacgg
     2161 catcgatatg cccacgcgca gcgaactgat tgcgcatggc cgcagtgagg aggaggtgtg
     2221 tcgcgagatc accgccgacg cgctggtcta ccaggacgtg gaggcactgc gccgctcgat
     2281 ttcggaagtc aatccgctgc tcaagcgctt cgaggcttcc tgctttgacg gcgtgtatgt
     2341 gaccggcgac gtgtcgccgg actacctgga gcgcatcgag gtcgcacgca acacaccgaa
     2401 gccgcaggcg gaggagggcg cgcgttccca gctcaacctc ggcctggcgc gcgccgactg
     2461 acgggcatta cgtccgctgc agcagcgagg caaactcgtt cgccggcagc ggacggctga
     2521 agtaatagcc ctgcatctcg tcgcagtcgt ggtcgcgcag gaagcgcacc tgttcctcgg
     2581 tttccacgcc ttcggcgatc accttcagat tcaggctgtg cccgagcgaa atcacggtgc
     2641 tggtgatggc cgcgtcgtcc acgtcgttgg cgatgtcccg cacgaaggac tggtcgatct
     2701 tcagatgatg cagcgggaat cgcttcagat agctgaggct ggaatagccg gtgccgaagt
     2761 cgtcgatcga cagctgcacg cccaggtctt gcagctcgcg cagcttgttc tcatagtgcc
     2821 cggcgccatt catcagcacg ctttccgtca gctccagttc aagatcctgc ggggccaggt
     2881 cgatgtgcgc cagggtgcgc tcgactgagc ccaccagccc ttcctgggcg aactggcgcg
     2941 cagacaggtt caccgatacg cagatgcgcg gcaggccggc ctgctgccat gccttgttct
     3001 gcgcgcaggc ggtcttcagg acccagtctc cgatcgggac gatcagcccg atttcctccg
     3061 ccatcggaat gaaacgcgtc ggcgaaacga gtccgagccg cggatggttc cagcggatca
     3121 gggcctcggc gccgatgatg cggcggctgc gcgcgtcgac cttgggctgg tagtgcagct
     3181 caaactcctt gcgttcgatc gccctgcgca gcgcgctttc cagctccatg cgttcgagcg
     3241 cgcgttcgct catctcgcgg gagtaaaaat gaaaggcgtt gccctgttcc tccttcgcgc
     3301 ggtacatcgc cgtgtcggcg ttgcgcagaa gcgactggat gtcgtcggcg tcgtcgggaa
     3361 agagcgtgat gccgatgctg gtgctgacgt acaggtcgcg cccttcgacc gtgaacgctt
     3421 ccgagaacgc gtcgaagatc ttgccggcaa ccgtggtgac atcttcaacc cggcgtactc
     3481 cgcagagcag gatgatgaac tcgtcgccgc cctggcgcgc caccgtgtcg ccctcgcgca
     3541 ccacctcgcg cagccgtgcg gcgacctgga gcagcagcgc atcgccgagg tgatggccga
     3601 agctgtcatt gacgcccttg aagcggtcga ggtcgaggaa cagcagcgcc acctgctcgc
     3661 cgctgcgacg agcgtgcgcc atcgcctggg tcaggcgatc cttgagcaga ttgcggttgg
     3721 ccaacccggt cagcgcgtcg tggtcggcga ggaattcgat tcttgcctcg taagccttgc
     3781 gcttgctgat gtcgatggcg acgccgtcca cgcgcagcag ctcgccgcgc gcattgcgga
     3841 tcgcacgtgc gcgttcctcg atccagcgta tttccccgtc aggccggacg atccggtatt
     3901 cctggctgaa cgtgccggat tcgggtagcc gtgcgtgcgc atcccgggct gcggcccggt
     3961 cttccggatg gattacatgg aaccacaagt gacggtccgc ataaaactca tcggccttgc
     4021 gtccgtagat gtgctcgacc atcgagttga tgtagatgaa ctcgcagtcg gtggccgacc
     4081 agacgacgtt gtcgatcgaa tcgaggatgc tggagagctt gtcctcggtc gctctcaggt
     4141 cccgttccgc cttttcgcgc gcggccttgg tgcgcaggct ggtcacgccg aaggcgatat
     4201 cccgtgccag gcccttcagc atgtcgacgt gctcacggtc gaaggcgttc gaatccgcct
     4261 cgtggatcgt cagcatgccg aacaccttgc cgttatggcg cagcggaagc gacatggata
     4321 cgccgtggtc cagagcaagc tcctgatgcg gcgcctcgtc gcccgctccc atttcgatga
     4381 ccagccagga ccgctcgctg tccaggttcc cgttcggccc ggcggcgccg ggattttgcc
     4441 ggacctggcg cggcacggcg ccggcgctgg cgacaacggt ctggctgccg tcctgttcgt
     4501 ccacatagct gatccaggcg gcctggtagc ggcctgcctc taccagggca gcgcaggtgc
     4561 cgttgagcag ttgagcctcg tcgccggcat gcaccatggc gtgattgcat tcagccataa
     4621 cggtgcgggc acgaagggtt cggcgcaagg cgtcctgcgc atgctggcga tcgccgactt
     4681 ccagcgccag tcggtcgtgc tgtttgcgga tctcgccata cagagacagg ttttcgtaga
     4741 tgacggccag ctgtgcggcc accgtgccgg cagcacgctc gtccacctcg ccgaagccct
     4801 cggcgccaag tttgtcggcc agatagagcc agccgaatac cttgtcctgc gagaccagcg
     4861 gcacgacgag cagcgagcgg attggcgggt gggcggccgg aaggccggca ggtcgcgccg
     4921 cgggctccag tccggaaagt cgaaggggcc ggcgctgagc aagcgcggtg ccgaatacgc
     4981 cgagacgcgg gttcatcgat gccacgccgg atgccgcgtc ctcgtccagt ccgcggaata
     5041 cccagtgacg cagtccatcg ccatcggcgt cgagaatacc gatgccggca tatttcgcga
     5101 ccgtcaggtc ctgcaccacc ctgcagccga tctgcagcag gcggtcggga tcgcgttcgg
     5161 tggatagctc gatgcccagt tcgatgagcg cagtcagacg aaggctgact gcctgcaggc
     5221 tggataacgc gttggacagg cgatcgacca cctgctgcag gtgaccggtg tcggtgcctt
     5281 cccggtcgcc gtcgcggacc agccgcgaca tcatgtgtct gctggtttcg acttcctcca
     5341 gataggcatt cagctggcgg ttcaggccgt cgaggcgctc gctcgaaggc gacagggcag
     5401 gttcggctgg ctcgggcagc tggtcggcag gcgtgcccag cgcctggtgg acggtctcaa
     5461 ggatcagggc cggttcagaa ggcttgggca gcacccagcg caccccgcag gcgagcgcca
     5521 tcgtggcggc ctcgcgctca tggtaggcgg cggtgtagaa gatgaccggc gtttcggcga
     5581 tttgcgcgat ggagcgcatg cgcgagacga actcgtagcc gtccatgttg ggcatcagga
     5641 tatcggcaat gacaaggtcg ggacagtgcc gttgcgcgag ttcgagccct tccgccccgt
     5701 ccttggcctc cagaaggcga tggccgccat tcccaaggag caccatcagg aattgccggt
     5761 ttaaaacatg gtcgtcaacg atcaggatgg tcgccatttc tgcttgtctt gtcgggttcc
     5821 aaggtgtcgt gtctttcgcg catgttctcc ttcggcagaa gctgtcccaa aatgctacct
     5881 ttctgtaaca cgcgcaggag actattcatt ccctcatttg attgcgatca cttgtgcggc
     5941 cagaagtgca attccggtac ggatttggac gacggctgtc ggatctccct gccgccgcgc
     6001 cggttttgcg ccgcagcgaa tttccgctac gatggctcct cgccatgcgc ggccctttcc
     6061 atctcctcac catgaccgac cacaagaaat acggcttcac cacgacgctg ctgcacagcg
     6121 accgcagaaa accaatcgaa cacggtgccc tccacaagcc tgtccacacg tccgtcacct
     6181 tcggctatgg cgatgcgcgc gaactggccg acgtattcca gggcaagaag cccggctacc
     6241 gctacgggcg ccagggcaat ccgaccgtca gcgcgctgga ggacaaggtc aatgcgatgg
     6301 aggaagggct cacgaccatc tgtttctcga ctggaatggc ggcagtcggc gcgctcgtgc
     6361 agggcttgct gcgaaccgga gatcacgtgg tctcttcggc attcctgttc gggaacacca
     6421 acagcctgtg gcagacggtg gatgcgcaag gcatgcccgt gagcttcgtc gacgcgaccg
     6481 acgcggaggc ggtggcagcg gcgatcacgc cggccacgcg cctggttttc gtggaaacca
     6541 ttgcgaaccc gcgcacccag gtcgcggacc tcaagcgcat cggcgagctg tgccgcgcgc
     6601 gcggcattct ctatgtcgtc gacaacacga tgacctcgcc ttacctgttc cgcccggcga
     6661 gcgtgggtgc cgggctggtc gtcaattccc tgaccaagtc cataggcggg cacggcaacg
     6721 ccctcggggg cagcctgacc gacacgggtc tatacgactg gacccggttt ccgaacatcg
     6781 cggacaacta caagcgtttc ccgagcgccc agtggggcat ggcgcaaatt cgcgcaaagg
     6841 cgttgcgcga cttcggtgcc gcgctcggtc ccgaggccgc gcatcatctt gcggttgggg
     6901 ccgaaaccat ggctttgcgg atggaacgca gttgcgcgaa cgcgcttgcg ctagcccgca
     6961 tgctggagca ggacgcacgt gtggcggccg tctactatcc cggtctcgtc tcacatccgc
     7021 aacaccagct cgcaagcgaa ctgttccgcg cacatggcta tttgttcagc ttcgagctgc
     7081 atgaagggct ggattgcttc gcctacctga accgcctgcg gcttgctgtt ccggccagca
     7141 atctcgggga caaccgcacc ctcgtcatac ccgttgctca cacgatcttc cacgaaatgg
     7201 gacccgagcg gcgcgccggc atgggcattg cggattccct gatccgggtg tcggtcggta
     7261 tcgaagacac ggacgacctg ctggaggatt tcagcgaggc tctggcgcta tagtttctac
     7321 ccggcactat cgtgccgaaa atctgctcgc ggcacctacg cgcatctcct acaatacgcc
     7381 gggggaattt tcccttcagc aaggagcggt tatgccacgg ccggaaaagg tgcgcctggg
     7441 tgagatcttg gtacagcaga agttgctttc ggacgagcaa cttcaattcg cactctctga
     7501 gcagaagcgt accggccgca agcttggccg cgtcttcatc gacaacggat tcgtcactga
     7561 agaacagatc tgcggcgccc tcgcgaagca gctcaacatt ccctacgtca atctgaagtt
     7621 cttcaacatc aagccggaaa tcgtgcgcct gctgccggaa acgcaggcgc gccgctttcg
     7681 tgcgctcgtg ctcgaggacc ggcacggcgc gctgctgatc ggcatggcgg acccgaccga
     7741 tctgttcgcc tacgacgaga tttcgcgcac cgtgcgtcgc ggcatcgagc ttgccgtcgt
     7801 caacgaaggc gaactgctgc agacgatcga ccgcatctac cggcgcaccg aggagatcac
     7861 cgatctcgca cgggagctgg aacaggatct cggcgagacc gcggtggatt ttggcgggct
     7921 cgccggcacg cccggcatag aggaggcgcc cgtcgtcaag ctgctg
//
LOCUS       DATLGF010000046         4106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_1075,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000046 DATLGF010000000
VERSION     DATLGF010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VM406_01525"
     CDS             complement(<1..827)
                     /locus_tag="VM406_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74669.1"
                     /translation="MKRTDDSAPCTALFRWLATLAAMLVALALLPPPTHAQLPGFPAA
                     TDAQAQDEAAPDQPEPIPLLEIPTQAAADLAEARRRQARPWIKDAIGLGASRLPRLEA
                     ALHGQRQGLEADFPAFQLEDLQRREERLRELEQQLVPVRRDLAAAALQLERDLRDLAR
                     LSARWAQTLEQAEPDMPPDLLARVRETVAAVQAAHRNLQDEQALVLSLQSRMADLATA
                     IDELREQYATAGGQAIRRLFRRDSAPLWSREFWLASVASFAPEANRHFREEHQRLSL"
     gene            complement(824..4051)
                     /locus_tag="VM406_01530"
     CDS             complement(824..4051)
                     /locus_tag="VM406_01530"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVL74670.1"
                     /translation="MSGQPDPGAAREPSEAVRKASTLARFFFLKTTFGILLAVTLVVG
                     GLFSYNLLVKEALPDLDIPQAMITTSWPGADPQTMERQVTDVIEDELATLQGVRSINS
                     ASFDSFSIISVEFQAGVNSVDAMARLRAAVSDAEADLPAEAERPAINQLSVDDRPILT
                     LALYGDADAATLSALVRSIRDRLERITGVNEVDLGGAREEIVQILLQPERLLALALSP
                     TQIRSAIQQANIEQPFGEIQSPDIGAVVRLEGRFRDLDDLRALPVATLGQGAAARTVR
                     LDEVATVQRTLEADQTRAFFSTGGQPYRPSTEVSVRKTPGADTVLLVEQIFATLSEMQ
                     QAPEWPQGVAFAAVQDDADQIWSSLSDVFVNGLQAMLVVFAVLFLAISWREGVIAGLS
                     IPVTFAGVLLAILLFGYSLNELVIIGMVIALGLIIDVFIIMVEGLHEDIYLNRRSFGE
                     SVLATVDRYAVPALAGQLTTVLALAPLMAIGGIAGEFIRVLPVTTITCLVLAFVVALL
                     ATLPLSRFLLGRVKVPEGEQAHSRADRVTVRAMDWLEAWNSRHVLSSRARAWLWVIGA
                     VLVFAISLFAIAQSRVELYPAQDGPRLGINIELPPTTQLAVSQQVADQAGEILRNKPY
                     LESTIKLVGRKSPFTAGSPADALQPTEAENFIGFSAIFVDRDLRDAPSYELADVLRDE
                     IAAYLERNVAGATLQVVAEAGGPSPGDPIEIQIIGPDMDTLRALSQQVQNLLRNTAGA
                     FNVRDNLGALNPEVALRPNREALEFFGISHAELAAQIRIAMSNDVIGTFTVAGGGDDI
                     DIRLGTEWATRPGEAGGPGSIEEIALVRAYTPAGESVSLFQLVRPLQSEGAVAIAHRD
                     GERALTVLARNQDRTVGEIMADVEPALRQMQAGWPPGYSFAIRGEAEEAEETFGAAGI
                     ALVVAIILVMGVLVIVFDSFRQSFIILATMPLALIGTFLGFFLFGISFSFFAMVGVIS
                     LVGIVVNNGIVMVDTMNRTLRQGVTIAEAAAAGSARRLRPVLTTSVTTIVGLIPLAVG
                     SPMYRPLTLVIIFGLVSATVLSLFVVPALYLLLTPEKHRHEESLD"
BASE COUNT          678 a         1409 c         1382 g          637 t
ORIGIN      
        1 tagaggctca gccgttggtg ctcttcgcgg aaatggcggt tcgcttctgg tgcaaagctg
       61 gcgaccgatg ccagccagaa ttccctgctc cacagcggcg cactgtcgcg ccggaacagc
      121 cggcggatcg cctgtccgcc ggctgttgcg tactgctcgc gcaactcgtc gatcgcggtg
      181 gcgaggtcgg ccatccggct ctgcagggac aggaccagcg cctgctcgtc ctgcaggttc
      241 ctgtgggctg cctggacggc agcgacggtt tcgcgtaccc gtgcgagcag gtcgggcggc
      301 atgtccggct cggcctgttc gagcgtctgt gcccatcgcg cggaaagccg ggccaggtca
      361 cgcaggtcac gttccagctg caaggccgcc gcagccagat ccctgcgtac cggcaccagt
      421 tgctgctcga gttcgcgcag gcgttcctcg cgccgctgca ggtcttcgag ctgaaatgcc
      481 ggaaaatccg cttccagccc ctggcgctgg ccgtgcaagg cggcctccag ccgcggcagg
      541 cgcgacgcgc ccaggccgat cgcgtccttg atccacgggc gcgcctggcg ccgtcgcgcc
      601 tcggccagat cggcggccgc ctgcgtcgga atctcgagca gcgggatcgg ctccggctga
      661 tccggcgcgg cctcgtcctg cgcctgcgcg tcggtcgcag ccgggaagcc cggcagctgg
      721 gcatgggttg gcggcggcag cagcgcgagc gcgaccagca tggccgcgag cgtggccagc
      781 cagcggaaca gcgcggtgca cggagctgaa tcatcggtgc gcttcaatcg agcgactcct
      841 catggcgatg tttttccggc gtcagcagca ggtacagtgc aggcaccacg aacagcgaga
      901 gcaccgtggc cgacaccagc ccgaagatga tgacgagggt caatggccgg tacatcgggc
      961 tgccgaccgc aagcgggatc aggccgacga tggtggtgac tgaggtcgtc agcacaggcc
     1021 gcagccggcg cgccgagccc gccgccgccg cttcggcgat cgtcacgcct tgcctgagcg
     1081 tgcggttcat cgtgtcgacc atcacgatgc cgttgttgac cacgatgccg accagcgaga
     1141 ttacgcccac catcgcgaag aacgagaacg aaattccaaa caggaagaag ccgaggaagg
     1201 tgccgatcag cgccagcggc atggtcgcga ggatgatgaa ggactggcgg aagctgtcga
     1261 acacgatgac cagcaccccc atcacgagga tgatcgcgac taccagcgca atcccggcgg
     1321 cgccgaaggt ctcctcggct tcctcggctt cgccgcggat cgcgaagctg tagccgggcg
     1381 gccagcccgc ctgcatctgg cgcaacgccg gctcgacatc ggccatgatc tcgcccacgg
     1441 tccggtcctg gttgcgtgca agcacggtca gcgcacgttc gccgtcccgg tgcgcgatcg
     1501 cgacggcgcc ctcggattgc agcggccgca ccagctggaa cagcgacacc gattcgccgg
     1561 ccggcgtgta cgcgcgcacc agtgcgatct cctcgatgct gccgggcccg cccgcttcgc
     1621 ccggtcgggt cgcccactcg gtgccgagcc ggatgtcgat gtcatcgccg ccgccggcaa
     1681 cggtaaaggt gccgatcacg tcattgctca tcgcgatgcg gatctgtgcc gccagctcgg
     1741 cgtggctgat gccgaagaac tccagcgcct cgcggttcgg gcgcagcgcc acctccgggt
     1801 tcagcgcgcc gaggttgtcg cgcacattga aggcccccgc ggtattgcgc agcaggttct
     1861 gtacctgctg ggacagcgcg cgcagcgtat ccatgtccgg gccgatgatc tggatctcga
     1921 tcgggtctcc gggcgagggg ccgcccgcct cggccaccac ctgcagtgtc gcgccggcga
     1981 cgttgcgttc gagataggcg gcgatttcgt cgcgcaggac atcggccagc tcgtacgacg
     2041 gggcgtcgcg caggtcgcgg tcgacgaaga tcgcggaaaa gccgatgaag ttttccgcct
     2101 cggtcggctg cagcgcatcc gcgggcgacc cggccgtaaa cgggctcttg cgcccgacca
     2161 gcttgatcgt gctttccaga tacggtttgt tgcgcaatat ttcgccggcc tggtcggcca
     2221 cctgctgcga caccgcgagc tgcgtggtcg ggggcagttc gatgttgatg cccagccgcg
     2281 gcccgtcctg cgccgggtag agttccacgc ggctctgcgc gattgcgaac agcgaaatgg
     2341 cgaagaccag caccgcaccg atcacccaca accatgcacg agcgcggctg gacagcacgt
     2401 ggcggctgtt ccaggcctcg agccagtcca tcgcccgtac cgtgacccgg tccgcgcggc
     2461 tgtgcgcctg ctcgccttcg gggaccttca ccctgccgag caagaagcgc gacagcggca
     2521 gcgtggccag cagcgcgacg acgaaggcca gcaccaaaca ggtgatggtc gtgaccggca
     2581 gcacgcggat gaactccccg gcgatgccgc cgattgccat caacggggcc agcgccagca
     2641 ccgtggtcag ctgtcccgcc agggcgggga ccgcgtagcg gtccaccgtc gccagcaccg
     2701 attccccgaa gctgcgcctg ttcaggtaga tgtcctcgtg caggccttcc accatgatga
     2761 tgaagacgtc gatgatcagg ccgagcgcga tcaccatgcc gatgatgacc agttcgttca
     2821 gcgaatagcc aaacagcagg atcgccagca gcacgccagc gaaagtcacg ggaatcgaca
     2881 ggccggcgat caccccctcg cgccaggaaa tggcgagaaa gagcacggcg aacaccacca
     2941 gcattgcctg cagtccgttg acgaacacat cgctcaacga ggaccagatc tggtcggcgt
     3001 cgtcctgtac ggcggcaaag gccacgccct gcggccactc cggcgcctgc tgcatctcgg
     3061 acagcgtcgc aaagatctgt tcgaccagca gcaccgtgtc ggccccgggc gtcttcctga
     3121 cggaaacctc ggtcgatggt cggtagggct ggccaccggt gctgaagaag gcgcgggtct
     3181 gatccgcttc cagcgtgcgc tggacggtgg cgacctcgtc cagccgcacc gtgcgcgcgg
     3241 cagcgccctg gcccagtgtg gccaccggca gcgcgcgcag gtcgtcgagg tcgcggaagc
     3301 ggccttccag ccgcaccacg gcgccgatgt ccgggctctg gatctcgccg aacggctgct
     3361 cgatgttggc ctgctggatc gcgctgcgga tctgggtggg ggagagcgcc agcgccagca
     3421 gccgctccgg ttgcagcagt atctgcacga tttcctcgcg cgcgccgccg agatcgacct
     3481 cgttgacgcc tgtgatgcgc tccagccggt cgcggatgct gcggacgagc gcgctcagcg
     3541 tggccgcgtc ggcgtcgccg tacagcgcga gcgtgaggat ggggcggtcg tccaccgaca
     3601 gctgattgat cgccggccgc tccgcttccg ccggcaaatc cgcctcggcg tcgctgaccg
     3661 ccgcgcgcag ccgcgccatc gcatcgacgg aattcacgcc ggcctggaac tcgacggaga
     3721 tgatggaaaa cgagtcgaag gaagcgctgt tgatcgaccg caccccttgc agcgtggcca
     3781 gctcatcctc gatcacgtcg gtgacctggc gctccatcgt ttgcggatcg gcgccgggcc
     3841 aggaagtggt gatcatcgcc tgcggaatgt ccaggtcagg caaggcctcc ttgaccagca
     3901 ggttgtagga aaacagcccg ccaaccacca gcgtcaccgc cagcaggatg ccgaacgtgg
     3961 tcttcaggaa aaagaagcgc gccagcgtcg aggccttgcg cacggcctcg ctcggctcgc
     4021 gggccgcgcc cggatccggc tgcccgctca tgacgccggc gcttgctgcc gggccgggtc
     4081 gagaatgcgc acgcgctgcc cgtcat
//
LOCUS       DATLGF010000047         5373 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_191040, whole genome shotgun sequence.
ACCESSION   DATLGF010000047 DATLGF010000000
VERSION     DATLGF010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191040"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VM406_01535"
     CDS             <1..253
                     /locus_tag="VM406_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HVL74671.1"
                     /translation="LLASAGLLEGRTMTSWPEIESDMRQVGANWVDQEVVVDGNLITS
                     RKPDDVPAFSQRLIEALQQRLQASISGTRDEYPEIGPQG"
     gene            complement(309..1067)
                     /locus_tag="VM406_01540"
     CDS             complement(309..1067)
                     /locus_tag="VM406_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HVL74672.1"
                     /translation="MDAFLNALRAELPSFWAAQVLATNLIVILNLLGALLLGLLVGYE
                     RSYRGRAAGMRTYGLVCMASAALTVVGGYPGEWFGGQTVLGIGADPTRIIQGIVTGVG
                     FLGAGVIMKEGFNISGLTTAASIWAASVIGVLVGVGFYLAAIVLALTAAICMIWVPKV
                     AGHLPSRQTIAITLRFKEGFTPSEQALSQSALARGYEIAPGSISIALAEGKVKWNFVA
                     VAFDRNAGAPISDLARELSEVEGVEGFHLSPARN"
     gene            complement(1084..1782)
                     /locus_tag="VM406_01545"
     CDS             complement(1084..1782)
                     /locus_tag="VM406_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HVL74673.1"
                     /translation="MSGDKPASKLPFKEALRQTIWAKVLTPEQMARVEADTVEQFVPK
                     GAYVCRKGEMVDAWIGVIEGLVKMNNLSREGKSVTFTGLSAGGWFGEGTLLKQEARKY
                     DVTALRDSRIARMPARTFNWLLDSSLPFTRFLLMQLNERLGQFIGLVEFDRMLDVDAR
                     VARCLAQMFNATLYPGTETLLQISQEEIGYLSGASRQRANQALQLLEKEGLLKVDYGG
                     IQILDIEGLRRFGA"
     gene            2007..3986
                     /locus_tag="VM406_01550"
     CDS             2007..3986
                     /locus_tag="VM406_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVL74674.1"
                     /translation="MANTRPTAGDAEATFPRLLLQHAQMRPDRPAFREKDLGIWQTST
                     WKEVADEVRALACGLAAMGFKRGMNLAIIGDNRPRLYWAMAAAQCLGGVAVPLYQDAP
                     AADMAYVLGDAGIEFAVAEDQEQVDKLLEIKQDLGDAALISHIIYDDARGMRHYTQPE
                     LSSFEKVQELGRELERNNPDFFMSQVNAGRGDDVAVMLYTSGTTGKPKGVCQTHTSFI
                     AAATGGVGFDKLTERDDILSYLPMAWVGDHLFSFAQALVAGFTVNCPESGDTVMTDLR
                     EIGPTYYFAPPRVFENMLTTVMIRMEDAGYIKRKLFHYYMEVARRVGSDILDGKPVGL
                     LDRLNYMLGSLLVYGPLKNVLGLSRVRVAYTAGAAIGPDLFRFYRSIGVNLKQLYGAT
                     ETCAYVCLQPDGNIKFDSVGLPAPGVEVKLADNGEVLVRSASMLKEYYKRPDATAEVI
                     DAEGYFHTGDAGVFDEEGHLKIIDRAADVGKMLSGEMFAPNYIENKLKFFPFIKEAVA
                     FGNQREMVCAFINIDMEAVGNWAERRGIPYSGYTDLAGKRETYDLIAECVEKVNADLA
                     NEAMMSGTQIHRFLVLHKELDPDDDELTRTRKVRRRFISEKYKVLIDALYSGKTSQYI
                     ETQVKFEDGRQGMVAADLRIVDVKTFSTPPLKKAA"
     gene            3988..4776
                     /locus_tag="VM406_01555"
     CDS             3988..4776
                     /locus_tag="VM406_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL74675.1"
                     /translation="MNANRKIGEVILDLRNISLSFGGVKALTDISFDVREHEIRAIIG
                     PNGAGKSSMLNVINGVYRPQQGEIVYRGEHRRDMNTYAAAKSGIARTFQNIALFKGMS
                     VLDNIMTGRNLKMQTNFLLQALYWGPAQKEEIEHRKKVEEIIDFLEIQHIRKTPVSRL
                     PYGLQKRVELGRALAAEPQILLLDEPMAGMNVEEKQDMCRFILDVNDQFGTTIVLIEH
                     DMGVVMDISDRVVVLDYGKKIGDGTPDQVRNNQDVINAYLGVAH"
     gene            4786..>5373
                     /locus_tag="VM406_01560"
     CDS             4786..>5373
                     /locus_tag="VM406_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVL74676.1"
                     /translation="MDFAFFLEVLIGGLLAGVMYALVALGFVLIYKASGVFNFAQGAM
                     VFFAALTCVGLVDTFGLSLWIAIPLTMVAMVILGLATERIVLRPLVAQHEITLFMATI
                     GLTFFIEGLAQLIWGANVRRLDLGIEDQPIMWLMDNYNLMVSQFDVIAATICAALVAS
                     LAVFFSKTKIGRALRAVADDHQAALAVGIPLQQIWG"
BASE COUNT         1033 a         1716 c         1699 g          925 t
ORIGIN      
        1 cctgttggcc tcggccggcc tgctggaagg gcgcacgatg acgagctggc ctgaaatcga
       61 atccgacatg cgccaggtag gcgcgaattg ggtcgatcag gaagtggtgg tggacggcaa
      121 tctgatcacc agccgcaagc cggacgacgt ccccgccttc agccagcgcc tgatcgaggc
      181 cctccagcag cggctgcagg ccagcataag cggcacccgc gatgaatatc cggagattgg
      241 tccgcagggg tgatggtgtg gccgggtgat gccgggacgt cgtcccggcg tacgtgccgg
      301 gctgtcgctc agttgcgcgc cggggacagg tgaaagccct cgaccccctc gacctcggac
      361 agctcgcgcg cgagatcgct gattggagcg ccggcgttcc ggtcgaaagc caccgcgacg
      421 aaattccact tgaccttgcc ttcggccagc gcgatcgaga tcgaccccgg cgcgatctcg
      481 tagccgcgcg ccagcgccga ctgcgacaac gcctgttcgc tcggggtgaa accttccttg
      541 aaacgcagcg tgatcgcaat ggtctggcgc gacggcagat ggccggcgac cttcggcacc
      601 cagatcatgc agatcgcagc cgtcagcgcc agcacgatgg ccgccagata gaagcccacg
      661 ccgaccagca cgccgatcac cgacgcggcc cagatcgagg cggcggtggt cagcccgctg
      721 atgttgaagc cttccttcat gatgaccccg gcgccgagaa agccgacgcc ggtgacgatg
      781 ccctggatga tgcgggtcgg atcggcgccg atgccgagca cggtctggcc gccgaaccat
      841 tcgcccgggt agccgccgac caccgtcagc gcggccgagg ccatgcagac cagcccgtag
      901 gtgcgcatgc ccgccgcgcg cccgcggtac gagcgctcgt agccgaccag caggcccagc
      961 aacagggccc cgagcaggtt gagaatgacg atcaggttgg tggccagcac ctgcgccgcc
     1021 cagaacgagg gcagctcggc gcgcaaggcg ttcaggaatg catccatgca gcagggaaaa
     1081 gaattaagct ccgaagcggc gcaggccctc gatatcgaga atctggatgc cgccgtaatc
     1141 gaccttcagc agaccttctt tctccagcag ctgcaaggcc tggttcgcgc gctggcgtga
     1201 agcgccggaa aggtagccga tctcttcctg agagatctgc agcagggttt cggtccccgg
     1261 gtacagcgtg gcgttgaaca tctgcgcgag gcagcgggcg acgcgggcat cgacatcgag
     1321 catgcggtcg aactctacca gcccgatgaa ctggccgagg cgctcgttga gttgcatcaa
     1381 caggaaccgg gtaaaaggca ggctgctgtc gagcagccag ttgaaggtac gcgccggcat
     1441 gcgcgcgatg cggctgtcgc gcagcgcggt cacgtcgtac ttgcgcgcct cctgcttgag
     1501 cagcgtgcct tcaccgaacc agccgccggc ggacaggccg gtaaacgtca ccgacttgcc
     1561 ttcgcgggaa aggttgttca tcttcaccag cccttcgatc acgccgatcc atgcatcgac
     1621 catctcgccc ttgcggcaca cgtatgcgcc cttgggtacg aactgctcga ccgtgtcggc
     1681 ctccacgcgc gccatctgct cgggtgtgag gaccttggcc cagatcgttt gccgcagcgc
     1741 ctccttgaaa ggaagtttgc tggctggctt gtctccgctc attgtgcttt gcaacaccga
     1801 tagtaatgat ttttttatgg attgtcggcg aaacgacagc atcgcaggtg cgcgctgtct
     1861 agtatgctga caactataag aatgacggag acattgtaag ccctagtcga ttgccgcggc
     1921 actaccgtgc gcaaggctct cgcttgcccg cccctgcgcg cgctctccgc ggaaaaacat
     1981 tcaccaattc acagagagtg ggacgaatgg caaacacgag accgacggcg ggcgacgcag
     2041 aagcgacctt cccccgcctg ctgctgcagc acgcgcaaat gcgccccgac aggccggcct
     2101 tccgcgagaa ggaccttggc atctggcaaa cctcgacctg gaaagaagtc gccgacgaag
     2161 tgcgtgcgct tgcctgcggg ctggcggcca tgggcttcaa gcgcgggatg aatctcgcga
     2221 tcatcggcga caaccgtccg cgcctgtact gggcgatggc ggccgcccag tgcctgggcg
     2281 gcgtcgcggt gccgctttac caggacgcgc cggcagccga catggcctac gtgctcggcg
     2341 acgccggcat cgaattcgcg gtggccgaag accaggaaca ggtggacaag ctgcttgaga
     2401 tcaagcagga cctcggcgac gcggcgctga tctcccacat catctacgac gacgcgcgcg
     2461 gcatgcgcca ctacacgcag ccggaactgt cgtcgttcga gaaggtgcag gaactcgggc
     2521 gcgaactcga gcgcaacaac ccggatttct tcatgagcca ggtcaacgcc ggacgcgggg
     2581 acgacgtggc cgtgatgctc tacacctcgg gcaccaccgg caagccgaag ggcgtgtgcc
     2641 agacgcacac ctcgttcatt gccgcggcca caggcggcgt cggcttcgac aagctcaccg
     2701 agcgggacga catcctgtcc tacctgccaa tggcctgggt gggcgaccac ctgttctcgt
     2761 tcgcacaagc gctggtggcc ggtttcacag tcaattgccc ggagtccggc gacacggtga
     2821 tgaccgacct gcgcgagatc gggccgacct actacttcgc gccgccgcgc gtcttcgaga
     2881 acatgctgac caccgtgatg atccgcatgg aagacgctgg ctacatcaag cgcaagctgt
     2941 tccattacta catggaggtc gcgcgccgcg tcggctccga tatcctggac ggcaagccgg
     3001 tcggcctgct ggaccggctg aactacatgc tcggcagcct gctggtctac ggcccgctga
     3061 agaacgtgct cggcctgtcg cgcgtgcgcg tggcctacac cgcgggcgcg gcgatcggcc
     3121 cggacctgtt ccgcttctac cgctcgatcg gcgtgaacct caagcagctg tacggcgcca
     3181 ccgaaacctg tgcgtatgtg tgcctgcaac cggacggcaa catcaagttc gacagcgtcg
     3241 gtctgccggc gccgggcgtc gaggtcaagc tggccgacaa cggtgaagtg ctggtgcgct
     3301 cggcctcgat gctcaaggag tactacaagc ggcctgacgc caccgcggaa gtcatcgacg
     3361 cggagggcta cttccatacc ggcgacgccg gcgtgttcga cgaggaaggc cacctgaaga
     3421 tcatcgaccg cgcggccgac gtcggcaaga tgctgtcggg cgagatgttc gcgccgaact
     3481 acatcgagaa caagctgaag ttcttcccgt tcatcaagga agcggtcgcc ttcggcaacc
     3541 agcgcgaaat ggtctgcgcg ttcatcaaca tcgacatgga agcggtcggc aactgggccg
     3601 agcggcgcgg catcccgtat tccggctaca ccgacctcgc cggcaagcgc gaaacctacg
     3661 acctgatcgc ggaatgcgtg gagaaggtca atgccgacct tgcgaacgaa gcgatgatgt
     3721 cgggcaccca gatccaccgc ttcctggtcc tgcacaagga actcgatccg gacgatgacg
     3781 agctgacccg tacccgcaag gtgcgccgcc gcttcatctc cgagaagtac aaggtgctga
     3841 tcgacgcgct ttattccggc aagacttcgc agtacatcga gacccaggtc aagttcgaag
     3901 acggccgcca gggcatggtt gcggccgacc tgcgcatcgt cgatgtgaag acgttctcca
     3961 ctccgccact gaagaaggcc gcctgacatg aatgcaaacc gcaagatcgg cgaagtcatc
     4021 ctcgacctgc gcaacatctc gctgtccttc ggcggcgtca aggctctgac cgacatttcc
     4081 ttcgatgtgc gcgagcacga aattcgcgcc atcatcggcc ccaacggggc cggcaagagt
     4141 tcaatgctca acgtgatcaa cggcgtctac cgtccgcagc agggagagat cgtctatcgc
     4201 ggcgagcacc ggcgcgacat gaacacctac gcggctgcca agagcggcat cgcgcgcacc
     4261 ttccagaaca tcgcgctgtt caagggcatg agcgtgctgg acaacatcat gaccggccgc
     4321 aatctgaaaa tgcagaccaa cttcctgctg caggcgctgt attggggccc ggcgcagaag
     4381 gaagaaatcg agcaccgcaa gaaggtcgag gaaatcatcg atttcctcga gatccagcac
     4441 atccgcaaga caccggtctc gcgcctgccg tatggcctgc aaaagcgcgt cgagctgggc
     4501 cgtgcgctgg ccgccgagcc gcagatcctg ctgctggacg agcccatggc cggcatgaac
     4561 gtggaagaaa agcaggacat gtgccgcttc atcctcgacg tcaacgacca gttcggcacc
     4621 accatcgtgc tgatcgagca cgacatgggc gtggtgatgg acatctcgga ccgcgtggtc
     4681 gtgctcgact acggcaagaa gatcggggac ggcacgccgg atcaggtgcg caacaaccag
     4741 gatgtgatca atgcctatct tggcgtggcc cactgaggac ctatcatgga tttcgctttt
     4801 tttctcgaag tactgatcgg cggcctgctc gccggcgtga tgtacgcgct ggtcgcgctc
     4861 ggtttcgtgc tgatctacaa ggcctcgggc gtgttcaatt tcgcgcaggg cgcgatggtg
     4921 ttcttcgccg cgctgacctg cgttggcctg gtcgatacct tcggcctgtc gctgtggatc
     4981 gcgattccgc tcacgatggt cgcgatggtg atcctggggc tggccaccga acgcatcgtg
     5041 ctgcggccgc tggttgccca gcatgagatt acgttgttca tggccaccat cggcctgacc
     5101 tttttcatcg aaggtctggc gcagctgatc tggggcgcga acgtgcgccg gctcgacctg
     5161 ggcatcgagg atcagccgat catgtggctg atggacaact acaacctgat ggtgtcgcag
     5221 ttcgacgtga tcgccgcgac catctgcgcg gcgctggtcg ccagtctcgc ggtgttcttc
     5281 tcgaagacca agatcggccg cgcgctgcgc gcggtggccg acgatcacca ggcggcattg
     5341 gcggtcggca ttccgctgca gcagatctgg ggc
//
LOCUS       DATLGF010000048         5606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_18373,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000048 DATLGF010000000
VERSION     DATLGF010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18373"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1888
                     /locus_tag="VM406_01565"
     CDS             <1..1888
                     /locus_tag="VM406_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HVL74677.1"
                     /translation="NGDNPGVPDAKAALDGARQILMERFAEDASLLQALREYLNEHGV
                     VESKVVDGKQDAGAKYADYFDYSEPISAIPSHRALALFRGRREEMLNVALRLDSEEDK
                     PKWDAPHNPCEGRIASRFGVSNQGRPADKWLSDTVRWAWRVKIFMHLETELMTALRER
                     AETEAINVFARNLKDLLLAAPAGPRVTIGLDPGLRTGVKVAVVDATGKVVDTATIFPH
                     QPRNDWDGSIQTLALLARKHEVGLVAIGNGTASRETDKLAQELIKRHPELKLTKIVVS
                     EAGASVYSASEFASRELPELDVSLRGAVSIARRLQDPLAELVKIDPKSIGVGQYQHDV
                     SQSQLARSLDAVVEDCVNAVGVDVNTASAPLLARVSGLNASVAQSIVNYRDMKGMFRS
                     RAALRDVPRLGEKTFEQAAGFLRIMNGDNPLDASAVHPESYPVVERILSDIKKDVKAV
                     IGDGKLLKSLSPARYADERFGVPTVNDILKELEKPGRDPRPEFTTATFKDGVEDIKDL
                     QPGMILEGVVTNVAAFGAFVDIGVHQDGLVHISALSNTFVRDPHTVVKAGQVVKVRVL
                     EVDEKRRRIALTMRLDDKAPAPGSRNEQRGDRNDARRLAQHQQGRQAAPAGAMAAAFA
                     KLRS"
     gene            1959..2270
                     /gene="grxD"
                     /locus_tag="VM406_01570"
     CDS             1959..2270
                     /gene="grxD"
                     /locus_tag="VM406_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HVL74678.1"
                     /translation="MSDVQTWIKETVTQHPVVLFMKGTAQFPQCGFSGRAVQILNACG
                     VENIITVNVLEDPEVRQGIKEYSNWPTIPQLYVNGELVGGCDIMTEMYQSGELQTLLK
                     G"
     gene            2290..2871
                     /locus_tag="VM406_01575"
     CDS             2290..2871
                     /locus_tag="VM406_01575"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HVL74679.1"
                     /translation="MASKVLIVAITGATGAVYGVRLLEVLRELGGVETHLVMSDAGVL
                     SLHQETGMSRKEAEALADVVYSVREVGAAIASGSFRCDGMVVAPCSMKTLAAIAHGLS
                     DNLISRAADVMLKERRRLVLMARETPLNLAHLRNMTAVTEMGAIVFPPLPAFYQRPQN
                     VQELVDHSLARVLDLLDIDQALAPRWTGMQGTP"
     gene            complement(2944..3019)
                     /locus_tag="VM406_01580"
     tRNA            complement(2944..3019)
                     /locus_tag="VM406_01580"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2984..2986),aa:Thr,seq:tgt)
     gene            complement(3054..3530)
                     /locus_tag="VM406_01585"
     CDS             complement(3054..3530)
                     /locus_tag="VM406_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HVL74680.1"
                     /translation="MSEIPTKPYLLRAIYEWCTDNGYTPYLASAVDTRTQVPREFVKN
                     GEIVLNISFTATSGLKLGNELIEFHARFGGVSREISIPVENVVAIYARENGQGMAFEV
                     ARREGEGEEAAEEKAGPALSAVPSPQNGEDADGDGGPGGSDDPPKKGGRPALTRIK"
     gene            complement(3569..4180)
                     /locus_tag="VM406_01590"
     CDS             complement(3569..4180)
                     /locus_tag="VM406_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HVL74681.1"
                     /translation="MMVLYSGTTCPFSQRCRLVLFEKGMDFEIRDVDLFNKPEDISVM
                     NPYGQVPILVERDLVLYESNIINEYIDERFPHPQLMPADPLMRARARLMLFNFEKELF
                     IHVHTLENDKAKGAEKLQDKARAEIRDRLTTLAPLFLKNKYMLGDEFSMLDVAIAPLL
                     WRLDHYGIELSKTAAPLMKYAERIFSRPAYIEALTPSEKVMRR"
     gene            complement(4299..5018)
                     /locus_tag="VM406_01595"
     CDS             complement(4299..5018)
                     /locus_tag="VM406_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HVL74682.1"
                     /translation="MKLMKKLIAALAFVPMLAIAAEATFPLDTAPRRTTDMAALQNGA
                     KLFVNYCLNCHSASAMRYNLLFTSDKVGDLMTIAMSRRDAATWFGAAPPDLSVIARAK
                     SSHDGPGADWLYTYLRTFYKDDTRELGWNNLVFPNVGMPHAMWELQGIREAVFEEVKD
                     PHNPNATIEKFVGFRQVRPGTMTPLDFDHATADLVAYLEWMAEPAQNKRKQLGVIVLL
                     FLFGFCVLTWLLNKTYWKDIK"
     gene            complement(5040..>5606)
                     /locus_tag="VM406_01600"
     CDS             complement(5040..>5606)
                     /locus_tag="VM406_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HVL74683.1"
                     /translation="GGYFLEYNNFIPADPLKTPPHIAPVWYFTPFYSILRATTSAFMW
                     ALIVGVIGYVLLIVLTTRLSSKIKIATAVIGLVTVIAMLVFDAKFWGVVLMGVSVLIL
                     AGLPWLDRSPVRSIRYRPNWHKWVYVVFGISFLILGYLGVLPPTEGRTLVSQVCTFIY
                     FGFFLLMPWWSAMGTFKPVPERVTFQPH"
BASE COUNT         1067 a         1760 c         1793 g          986 t
ORIGIN      
        1 aaacggcgac aaccccggcg tgccggatgc caaggccgcg ctcgacggcg cccgccagat
       61 cctgatggag cgcttcgcgg aggacgccag tctgctgcag gccttgcgcg agtatctgaa
      121 cgagcacggt gtggtcgaat cgaaggtggt cgacggcaag caggatgcgg gagcgaagta
      181 tgccgactac ttcgactact cggaaccgat ctcggccatt ccgtcccacc gtgcgctggc
      241 cttgttccgg gggcgccgcg aagaaatgtt gaacgtggcg ctgcgcctgg acagcgagga
      301 agacaagccg aaatgggacg cgccgcacaa cccctgcgag ggccgcatcg cgagccgatt
      361 cggcgtcagc aaccagggcc gccctgccga caagtggctg tcggacaccg tgcgctgggc
      421 ctggcgcgtg aaaatcttca tgcatctgga aaccgagctg atgaccgcgc tgcgcgaacg
      481 cgcggagacc gaggcgatca acgtgtttgc ccgcaacctg aaggacctgc tgctggcggc
      541 gcccgccggc ccgcgcgtga ccatcggtct cgatccgggc ctgcgcaccg gcgtgaaggt
      601 cgcggtcgtc gatgcgacgg gcaaggtggt cgataccgcc acgatctttc cgcatcagcc
      661 gcgcaacgac tgggacggct cgatccagac cctggcgctg ctggctcgca agcatgaagt
      721 cgggctggtt gcgatcggca acggcaccgc ctcgcgcgag accgacaagc tggcgcagga
      781 gctcatcaag cgccatcctg aactcaagct gaccaagatc gtcgtgtccg aggcgggcgc
      841 gtcggtgtac tccgcgtccg agttcgcgtc gcgcgaactg ccggagctcg atgtctcgct
      901 gcgcggcgcg gtctcgatcg cccggcgcct gcaggacccg ttggccgaac tggtgaagat
      961 cgacccgaag tcgatcggcg tcggccagta ccagcacgat gtcagccagt cccagctggc
     1021 gcgctcgctg gatgcggtcg tcgaggattg cgtgaacgcc gtcggcgtgg acgtcaatac
     1081 cgcctcggcg ccgcttcttg cgcgcgtgtc cggcctcaat gccagcgtcg cgcaaagcat
     1141 cgtcaattac cgcgacatga agggcatgtt ccgttcgcgc gccgcgctgc gggacgtgcc
     1201 acgactgggc gagaagacct tcgagcaggc ggcgggcttc ctgcgcatca tgaatggcga
     1261 caacccgctc gacgcctcgg ccgtgcaccc ggaatcctat ccggtggtcg agcgcatcct
     1321 gtccgacatc aagaaagatg tgaaggccgt gattggcgac ggcaagctgt tgaagtcgct
     1381 cagccccgcc aggtatgcgg acgagcgctt cggtgtgccc actgtcaacg atatcctgaa
     1441 ggaactggaa aagccgggac gcgatccgcg gccggagttc accaccgcga cgttcaagga
     1501 tggcgtcgaa gacatcaagg atttgcagcc gggcatgatt ctcgagggcg tggtgaccaa
     1561 tgtcgccgca ttcggcgcct tcgtcgacat cggcgtgcac caggatgggc tggtgcatat
     1621 ttccgcgctg tcgaacacct tcgtgcgcga cccgcacacg gtcgtgaaag ccggccaggt
     1681 ggtcaaggtc cgggtgctcg aagtcgacga gaagcgccgg cggattgcgc tgacgatgcg
     1741 gctggacgac aaggcgccgg cacccggcag ccgcaacgag cagcgcggcg accgcaatga
     1801 cgcgcgccgg ctcgcccagc accagcaggg ccgccaggcg gctccggccg gcgcgatggc
     1861 ggcagcgttc gcgaagctgc gttcctgaaa gccaggccgg tccggggccg gccttttccc
     1921 tataatgagg gttttccccc atacctgaag gcagcaccat gagcgatgtc caaacctgga
     1981 tcaaggaaac cgtcacccag caccctgtcg tgctgttcat gaaaggcacc gcccagtttc
     2041 cgcaatgcgg cttttccggc cgcgccgtgc agattctcaa cgcctgcggc gtcgagaaca
     2101 tcatcaccgt caatgtcctc gaagacccgg aagtccgcca agggatcaag gaatattcga
     2161 actggccgac tatcccgcag ctgtatgtga acggcgaatt ggtcggcggc tgcgacatca
     2221 tgaccgagat gtaccagtcg ggcgagctgc agaccctgct caagggctga cgaggttcgc
     2281 gcgacgcgca tggcgtccaa ggtcctcatc gtcgccatca cgggcgccac cggcgccgtc
     2341 tacggcgtgc gtctgctcga agtgctgcgc gaactcggcg gtgtcgaaac ccatctggtg
     2401 atgtccgatg ccggtgtgct cagcctgcac caggaaaccg ggatgagccg caaggaggcc
     2461 gaggcgctgg ccgacgtcgt ctacagcgtg cgcgaagtcg gcgccgcgat tgccagcggc
     2521 tccttccgct gcgacggcat ggtggtcgcg ccctgctcca tgaagacgct ggccgccatc
     2581 gcgcacggac tgtcggacaa cctgatctcg cgcgccgcgg acgtgatgct gaaggagcgc
     2641 cgccggctcg tgctgatggc gcgcgaaacc ccgctgaacc tggcccacct gcgcaacatg
     2701 acggccgtca ccgagatggg cgcgatcgtc tttccgccgc tgcctgcctt ctatcagcgc
     2761 ccgcaaaacg tgcaggaact tgtcgatcac tcgctagccc gcgtgctcga tctgctcgac
     2821 attgatcagg cgcttgcgcc gcgctggaca ggcatgcaag gcaccccttg aagcgcaggc
     2881 tcggaccggc aagccttggc ccaaatgaaa aagcccatga ccgaggtcat gggctttttg
     2941 ttttggtgcc ggctgcagga ctcgaacccg ccacctgatg attacaaatc aactgctcta
     3001 ccagatgagc taagccggcg aaagctcgct attgtacgcg attcggctgc actttatttg
     3061 attcgtgtaa gcgccggacg cccgcccttc ttcggcggat cgtcgctgcc gccgggacca
     3121 ccatccccat cggcgtcctc gccgttctgt ggcgacggca cggcggagag cgccgggccg
     3181 gccttttcct cggccgcttc ctcaccttcg ccttcgcggc gcgcgacctc gaaggccatg
     3241 ccctgcccgt tctcacgggc gtagattgcg acgacgttct cgaccgggat tgaaatctct
     3301 cgagagacgc cgccgaagcg ggcgtgaaac tcgatgagtt cattgccgag cttcagtccg
     3361 ctcgtcgctg tgaagctgat gttgaggacg atttcgccat tcttcacgaa ctcgcgcggc
     3421 acctgcgtgc gggtgtcgac cgcgctcgcg aggtagggcg tgtagccgtt gtcggtgcac
     3481 cactcgtaaa tggcgcgcaa caggtaaggc ttggtaggaa tctcggacat ggctgtttgc
     3541 tttgtcgcct tgccgcggcg acacggcctc agcggcgcat cactttctcg gaaggcgtca
     3601 gggcttcgat atatgcggga cgggagaaaa tgcgttcagc atacttcatc aggggcgccg
     3661 ccgtcttcga caactcgatg ccgtaatggt ccaggcgcca gagcaacggt gcgatcgcca
     3721 cgtcgagcat cgagaattcg tcgccaagca tgtacttgtt cttcaggaac agcggagcaa
     3781 gcgtggtcag gcggtcgcga atctctgcac gggccttgtc ttgaagtttt tccgcgccct
     3841 tggccttgtc gttttccaag gtgtggacgt gaatgaacaa ctccttttca aagttgaaca
     3901 gcatcaggcg ggcacgcgcg cgcatcagcg gatcggccgg catcagttgc ggatgcggga
     3961 aacgctcgtc gatgtactcg ttgatgatgt tcgactcgta caacaccagg tcgcgctcaa
     4021 cgagaatcgg cacctgcccg taggggttca tcacggaaat gtcttccggc ttgttgaaca
     4081 ggtcaacatc ccggatttcg aaatccatgc ccttttcgaa caggacgagg cggcaacgct
     4141 gggaaaatgg gcaggtcgtg cccgagtaga gaaccatcat tttgcagttc cttggaaaca
     4201 aaggggtgag acgtgcaacg gctcaccccg agactagcgc gacaccccga gcggatcagc
     4261 catcgcgccg gcagatccgc gggggcaatg caagtgcttt acttgatgtc tttccagtac
     4321 gtcttgttca gcaaccaggt cagcacgcag aaaccgaaca ggaacaacag cacgatcaca
     4381 ccgagctgct tgcgcttgtt ctgcgcgggt tcggccatcc actccaggta agcgaccagg
     4441 tccgcggtgg cgtgatcgaa gtcgagcggg gtcatcgtgc cgggtcgcac ctggcggaag
     4501 ccgacgaact tctcgatcgt cgcattcggg ttgtgcggat ccttcacttc ctcgaagacc
     4561 gcctcgcgaa tgccctgcag ttcccacatc gcatgcggca tgccgacgtt cgggaacacc
     4621 aggttgttcc agcccagttc gcgcgtgtcg tccttgtaga acgtgcgcag ataggtgtac
     4681 agccagtccg cgcccggacc gtcatgcgac gacttcgcgc gggcgatgac ggacagatcc
     4741 ggtggtgcgg cgccgaacca ggtggcggcg tcgcggcgcg acatcgcgat ggtcatcagg
     4801 tcaccgacct tgtcggacgt aaacagcagg ttgtagcgca tcgcggacgc cgagtggcag
     4861 ttcaggcagt agttcacaaa cagcttggcg ccgttctgca gcgccgccat gtccgtggtg
     4921 cgacgcggtg ccgtatcgag cgggaaggtc gcttcggccg caatcgcgag catcggcacg
     4981 aaagccaggg ccgcaatcag tttcttcatc agtttcatga ttctctattc ctctgcggct
     5041 tagtgcggct ggaaggtcac gcgctcgggg accggcttga aggtacccat cgcgctccac
     5101 cacggcatca gcaggaagaa gccgaagtag atgaaagtgc acacctgcga caccaaggtg
     5161 cggccttcgg tcggcggcag cacgccgaga tagccgagga tcaggaacga gatgccgaac
     5221 accacataga cccacttgtg ccagttcggg cgatagcgga tcgagcgcac cggcgagcgg
     5281 tccagccacg gcagaccggc caggataagc acggacacgc ccatcagcac cacgccccag
     5341 aacttcgcgt cgaacaccag catcgcgatg acggtcacca ggccgatcac ggccgtggcg
     5401 atcttgatct tcgacgacag gcgggtggtc aggacgatca gcagcacgta gccgatcacg
     5461 ccgacgatca gcgcccacat gaacgccgag gtggtcgcgc gcaggatcga gtagaacggc
     5521 gtgaagtacc agaccggcgc gatgtgcggc ggcgtcttca gcgggtcggc cgggatgaag
     5581 ttgttgtact cgaggaagta gccgcc
//
LOCUS       DATLGF010000049        18526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_206987, whole genome shotgun sequence.
ACCESSION   DATLGF010000049 DATLGF010000000
VERSION     DATLGF010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 18526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18526
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206987"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="VM406_01605"
     CDS             complement(<1..931)
                     /locus_tag="VM406_01605"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HVL74684.1"
                     /translation="MQVILLHHRFSAKTVTLTRWHLTAAAAGFLVLVLGAASLLYALT
                     LRYAAQLDIPVVRDALANAAVGDPAKQDQYIKENLAAMAIRIGEMQAQLMRLDALGER
                     VQGLAGVKPQEFNFREPPPRGGAHPSVGEPLSMSEFQQSLDALARDVEYRADYMNVVE
                     STLMGYKIRSKLLPTVHPVNVAYNASGFGWRIDPFTGRSARHEGIDFAAPAGTPIVAA
                     AGGVVIAAHYHPQYGNMLDIDHGNDIVTRYAHASRLHARVGDIVRRGQRVADVGSTGR
                     STGAHLHFEVLVKGVPQNPHKFLSAGADQARLAA"
     gene            983..1498
                     /locus_tag="VM406_01610"
     CDS             983..1498
                     /locus_tag="VM406_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HVL74685.1"
                     /translation="MRNSSFRSTSSLVRGARANTRGTREAADFLRSHDRMAALVSVAE
                     RLAALDRDCASLLPTGFSACRVLHLDGGQLVLAVPGAAWAAKLKQQLPRLQDGLVKRG
                     WQVNAIRLKVQGGNISLPTRPPKQLVMPATARQAFQELESSLDKSKGNEALKGALRAL
                     LERHGPGGSNR"
     gene            complement(1521..2456)
                     /gene="lpxC"
                     /locus_tag="VM406_01615"
     CDS             complement(1521..2456)
                     /gene="lpxC"
                     /locus_tag="VM406_01615"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HVL74686.1"
                     /translation="MLKQRTVKQQIKTIGVGLHSGTKVELTLRPAPPDTGIVFRRVDL
                     DPVVELPASAMGVGDTRMASVLQKDGARVSTVEHLMSACAGLGIDNLHVDVTAEEIPI
                     MDGSAATFVFLLQQAGLLEQAAPKRFIRVKRPVEVREGSGAQEKWARLEPYNGFRLRF
                     FIEFNHPAVDATGQVAEVDFEVESYVKEIARARTFGFMQDVETLRGMGLARGGSLENA
                     IVMDEYRVLNPDGLRYANEFVRHKILDAIGDLYLVGHPLLASYVAHKSGHAMNNQLLR
                     ALLAQPDAYEIVSFDSIEQAPRTYAQQAEEEWAYA"
     gene            complement(2535..4019)
                     /gene="glpK"
                     /locus_tag="VM406_01620"
     CDS             complement(2535..4019)
                     /gene="glpK"
                     /locus_tag="VM406_01620"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HVL74687.1"
                     /translation="MSSFILALDQGTTSSRAILFDRAGRMHGIAQQEFRQIFPQSGWV
                     EHDANEIWSSQLAVARQVMKETGVTAASIAAIGITNQRETVVLWDRKTGEPVANAIVW
                     QDHRTAELCDSLRSDGHEPLFREKTGLVVDAYFSGTKLKWLLDHVPGARERARRGELA
                     FGTVDSWLIFKLCGRHVIDTSNASRTLMFNIHTLRWDEELLELLDVPADLLPEVVPSS
                     GVVGHAEAACFGAAIPIAGIAGDQQAATFGQACHGCGMAKNTYGTGCFMLLNTGKRAM
                     SSRNNLLTTIGWTVNGVTEYMLEGAVFNAGAAVQWLRDGLGIIRESAEVEALATSVPD
                     TGGVFFVPAFSGLGAPYWDAYARGTLVGISRGTTRAHIARATLAGIAYQSADVLEAMQ
                     KDAAMEMTELRVDGGAARNDLLMQFQADVLGVPVVRPAVTETTALGAAYLAGLAVGFW
                     SSQDEIAAQWQVERRFEPAMSIDERAQRLATWRRAVERARAWVE"
     gene            complement(4190..5917)
                     /locus_tag="VM406_01625"
     CDS             complement(4190..5917)
                     /locus_tag="VM406_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL74688.1"
                     /translation="MRLKLTALAMASMLACGASWADMKAAERWVDQEFQPSTLNRAQQ
                     LEEMKWFIDAAAKLRARGVKEINVVSETIDTHVYESKTLAKAFEEITGIRVKHDLIQE
                     GDVVEKLQTSMQSGKSIYDGWISDSDLIGTHYRYGAIEPLSDYIAGKGKEFTNPNLDL
                     KDFIGLSFTTAPDGKVYQLPDQQFANLYWFRADLFADKVLQDRFKAKYGYELGVPLNW
                     SAYEDIADFFTNDVKEINGRRIYGHMDYGKKDPSLGWRFTDAWLSMAGAADKGIPNGL
                     PVDEWGIRVADDKCTPVGASVARGGATNSPAAVYALTKYVDWMKKYAPPEAIGMTFSE
                     AGPVPAQGQIAQQIFWYTAFTASMTKQGLPVVHPDGRPKWRMAPGPHGPYWKQGMQNG
                     YQDVGAWTFFRSTPDDRKAAAWLYAQFVNAKTTSLKKSIVGLTFVRDSDINHQYFTDN
                     AHKYGGLVEFYRSPARVAWTPTGNNVPDYPRLAQLWWKNVANAVTGERTPQQAMDTLA
                     EEMDQVMARLQRSGMKNCAPKLNPKSDPAKWLSDTGAPWKKLDNEKPKGQTIRYDVLL
                     QAWKEGRER"
     gene            complement(6003..6287)
                     /locus_tag="VM406_01630"
     CDS             complement(6003..6287)
                     /locus_tag="VM406_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2160 domain-containing protein"
                     /protein_id="HVL74689.1"
                     /translation="MQEAFAWMAWTTPVAIFFTAIVLMLIAMTVLELRSPTIMRKGFL
                     PIPTTRGDRLFIGLLSAAYVNLAWTGLTDLTQWVGAVLGLGVLWIVMRWG"
     gene            complement(6289..7095)
                     /locus_tag="VM406_01635"
     CDS             complement(6289..7095)
                     /locus_tag="VM406_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HVL74690.1"
                     /translation="MMSKHITRPLLALYLLLSLLPIYWMLNMSLKTNAEIVGTLTLWP
                     QNLTFDNFRTIFTDPSWYDGYINSIIYVLINMVLSVAIALPAAYAFSRYRFLGDKHLF
                     FWLLTNRMTPPAVFLLPFFQLYSTVGLMDTHVAVALAHMVFNVPLAVWILEGFMSGVP
                     KEIDETAYIDGYSFPAFFVKIFLPMIKAGVGVTMFFCFMFSWVELLLARTLTSVNAKP
                     IAATMTRTVSAAGMDWGVLAAAGVLTIVPGALVIWFVRHYIAKGFAMGRV"
     gene            complement(7092..7961)
                     /locus_tag="VM406_01640"
     CDS             complement(7092..7961)
                     /locus_tag="VM406_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HVL74691.1"
                     /translation="MKPYNNRAWFLILPVILAVAFSAILPLMTIVNYSVQDILGPGQS
                     VFVGTEWFQMVMRDSELHGALLRQILFSLSVLAIEIPLGILIALTLPAQGWRASLALV
                     LIALPLLIPWNVVGTIWQIFGRGDIGLLGYTINQIGFSYNYSANPIDAWVTVLAMDVW
                     HWTPLVVLLCYAGLRAIPDAYYQAAKIDGASRWAVFRFIQLPKLRNVLMIAVLLRFMD
                     SFMIYTEPFVLTGGGPGSTTTFLSQYLTTKAVGQFDLGPAAAFSLIYFLIILLFCFVL
                     YNWMQRVGTGDAK"
     gene            complement(7963..9045)
                     /locus_tag="VM406_01645"
     CDS             complement(7963..9045)
                     /locus_tag="VM406_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL74692.1"
                     /translation="MARIELVELAHAYKPNPTQPGDYALRPMTMTWDDGGAYALLGPS
                     GCGKTTLLNIISGLVRPSHGRVLFDGKDVTALPPEQRNIAQVFQFPVIYDTMTVFDNL
                     AFPLRNRGWGAAEVGRRVSQVAEMLDLTSDLKHRASGLSADAKQKISLGRGLVRPDVA
                     AVLFDEPLTVIDPHLKWLLRRKLKEIHHELKLSLIYVTHDQVEALTFADKVVVMTQGE
                     VVQAGSAQELFEEPAHTFVGYFIGNPGMNLLPCTVRDGAAYVEGNGIALSDKARAALK
                     GAAQATLGIRPEFVECASAPGQGAAQATVSTVRDLGTHFLAEFQVGARSVAAKLRTMH
                     AVPGETVWLRLPPERTLYYVNDKRVA"
     gene            complement(9033..10145)
                     /locus_tag="VM406_01650"
     CDS             complement(9033..10145)
                     /locus_tag="VM406_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL74693.1"
                     /translation="MQLTLENVSKKHGAEDHVYPLSLQLAPGTINVLLGTTLAGKTSL
                     MRLMAGLDRPTGGRVLEEGRDVTGVPVRERNLAMVYQQFINYPSLTVYENIASPLRLR
                     RLPEDQVRKRVTEMAERLHITPYLQRSPAELSGGQQQRTALARALVKEAGLLLLDEPL
                     VNLDYKLREELRRELSDLFADGKTTVVYATTEPLEALQLGGQVCVLHEGRLLQSGPTL
                     DVFRAPNCIQAARTFSDPPINLMPAVLQDDGRANLGNGLLLPLNPHQKRAAAGARDIV
                     LGLRAHSLRLTQRPGDATIDAEIELAEISGSETYVHVHRGRIELVAQLQGVHNLEIGL
                     PCSLHCQPDDLFVFARDGELLYAPNQYQQGGEAWRA"
     gene            complement(10278..11819)
                     /gene="glpD"
                     /locus_tag="VM406_01655"
     CDS             complement(10278..11819)
                     /gene="glpD"
                     /locus_tag="VM406_01655"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HVL74694.1"
                     /translation="MPRQLLECDLLIIGGGINGVGIARDAAGRGLSVVLCEKDDLGAH
                     TSSASSKLIHGGLRYLENREFGLVRKALIEREVLLRAAPHIMWPMRFVLPHDRGQRPA
                     WMIRTGLFLYDSLARRDLLPGSQSVDLRSHPAGDPLKAEFTRGFVYSDGWVDDARLVA
                     LSAVDAAAKGARILTRTACTAVERVGSRWSATLWGQDGAAWSVSARALVNAAGPWAAQ
                     VLELASKRGAARPLRLIKGSHIVVPRMFDHPYAYIFQHPDGRIVFAIPFQGEFTLIGT
                     TDIEYHGDAGTVAISSEEITYLCDLSARYFSRPVTPADVVWSYSGVRPLVEDEASDAS
                     SVTRDYKLELEAGTAPLLSVLGGKITTFRKLAEEAVDLIAPLLSARGERWTADACLPG
                     GDLYGSAPCKRAVLEFDSFVQALQQRYAWLPPVLMARYARAYGTRTELLLAGRNCIAD
                     MGDEVTSGLYEAEIDYLVEHEWARTAADILWRRTKLGLRAPPGAEAALDARLAQRVTR
                     RRTQA"
     gene            11997..12758
                     /locus_tag="VM406_01660"
     CDS             11997..12758
                     /locus_tag="VM406_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HVL74695.1"
                     /translation="MTLNPRQRQLLDMVRRKGTISVEELAQQLEVTPQTVRRDIKQME
                     EAKLLARYHGGVGVPSSVENIDYGQRQALNIEAKRRIAAAVAAQVPAGCSLLINIGTT
                     TEEVARALVHHEGLHVVTNNLNVAAILADNPHSEVIIAGGVVRARDRGIVGEATIDFI
                     RQFKVDIGIIGISSIEPDGTLRDYDPREVKVAQTIIEQSREVWLVADHDKFGRPALVR
                     LAHVSQIDMLFTDARPPEALARALKEARVKVVVAP"
     gene            complement(12828..13331)
                     /locus_tag="VM406_01665"
     CDS             complement(12828..13331)
                     /locus_tag="VM406_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HVL74696.1"
                     /translation="MTIKIGDRLPEATLYEFIDTETEGCTLGPNAFKVSDLVKGKKIA
                     IFALPGAYTPTCSAKHVPGYVEHADALKNKGVDEIWCISVNDAFVMGAWCREQKAGGK
                     VRMMADGSGEFTKAVGLELDLTAKGMGVRSQRYSMLVEDGVVKQVNIEAPGKFEVSNA
                     ETMLAQV"
     gene            complement(13508..14695)
                     /gene="ftsZ"
                     /locus_tag="VM406_01670"
     CDS             complement(13508..14695)
                     /gene="ftsZ"
                     /locus_tag="VM406_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HVL74697.1"
                     /translation="MEIDMIDNANLGTVIKVVGVGGAGGNAVQHMINKGVSGVEFIAA
                     NTDAQALAQSKAHNVIQIGDTGLGAGMKPEVGRKLAEESRARIEDALRGAHMVFIAAG
                     MGGGTGTGAAPIVAQVAKELGALTVAVVSKPFSYEGAKCMEIADEGLEALSQHVDSLI
                     VILNEKLEDIYEDDSMIEWLQHADDVLNNAVAGIAEIINVPGHINVDFNDVKTIMGEQ
                     GKAMMGTATASGVDRARIAAEQAVASPLLDGIDLSGARGVLVNVTASRSLKGKEIKEV
                     MATVRAFAAPDASIAQGIAYDDSMGDDIRVTVVATGLGRARKNVQLVQTPVLRTGTHN
                     EPIMADATVAQTATGAVGASFGGLKAPAVWRRESASETVRALEKNGMETYDIPAFLRK
                     QAD"
     gene            complement(14796..16028)
                     /gene="ftsA"
                     /locus_tag="VM406_01675"
     CDS             complement(14796..16028)
                     /gene="ftsA"
                     /locus_tag="VM406_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HVL74698.1"
                     /translation="MTKDAKNLIVGLDIGTSKVVAVVAEVLPNGRHEVIGLGQHESKG
                     LKKGVVVNIEATVESIQRALEEAELMADCKIRNVYTGIAGSHIRSFNSSGMVAIKDKE
                     VTPTDVARVIETAKAVNIPTDQQLLHTVPQEFIVDNQEDVREPIGMSGIRLEVKVHIV
                     TGAVSAVQNIVKCVRRCGLEVSDLILQPMASADAVLTPDEKELGVVLVDIGGGTTDVA
                     IFTEGAIRHTAVIPIAGDQITNDIAMALRTPTAEAEEIKLRFGVAKQVLADPNETLEV
                     PGLGDRGPRALSRQALAAVIEPRVEELFALVHQVVRESGYEEVLSSGIVLTGGSSTMP
                     GMVELAEDIFLKPTRLGVPEYSGQLADVVRSPRYSTVLGLLMEAKKQYLRGHLVTRQD
                     GSVGAVWKRMKEWFFGTF"
     gene            complement(16039..16806)
                     /locus_tag="VM406_01680"
     CDS             complement(16039..16806)
                     /locus_tag="VM406_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HVL74699.1"
                     /translation="MWHDVRTLNATTNALLGLLVLGLLASGFWWVAHRPMFTFRVVVV
                     EGAQGAPLRHVSAPTIRTTALPGVKGNFFTADLDAVRQAFESVPWVRQATVRREWPNR
                     LIVNVEEHRALGAWGDDGRLLSVKGEVFTANLAEAEADGPLPVFSGPEGSAKDVVARF
                     RELHEWLKPAGLVPESVALSERYAWTVKLVGGMTVELGREQARVSVQERAQRLVGIYP
                     QLAARLQDRIETVDMRYPNGLALSAQGLKLATEGKKK"
     gene            complement(16811..17782)
                     /locus_tag="VM406_01685"
     CDS             complement(16811..17782)
                     /locus_tag="VM406_01685"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HVL74700.1"
                     /translation="MGKEFGKVGVLYGGRSAEREVSLMSGSGVLKALQSKGIDAHGFD
                     TGERSIAELAAQKFDRAFIALHGRYGEDGTLQGVLEQLGIPYTGSGVMASAIGMDKVM
                     SKRIWINHGLPTPRFVVLDARDTTREQLRAVPDELGLPLMLKAPHEGSTIGIAKVAGY
                     SEMQNGFELCAKYDEVVLAEEFISGRELTVPVLGRGRHARALPIVEIRAPQGNYDYEH
                     KYFSDDTQYLCPAPLDEALTRRIQSLAVGAFNALGCAGWARVDFMLRATDNEPFLLEI
                     NTSPGMTGHSLVPMSAKADGVSYEDLCVEILRMATLELKPNTEWRPA"
     gene            complement(17786..>18526)
                     /locus_tag="VM406_01690"
     CDS             complement(17786..>18526)
                     /locus_tag="VM406_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HVL74701.1"
                     /translation="CLDDPNVREIMPFISKPIVTYGFHPEAEVRAVDAKAVDSHMEFT
                     VLQKDYPPLQVHLNQPGMHNVQNACAAIAIARELGVDDSATQKALKEFNGVGRRFTRY
                     GDVALPGGGAFTLVDDYGHHPVEMAATIAAARGAYPGRRLVLAFQPHRYTRTRDLFED
                     FVKVMSSVDALVLGEVYAAGEAPIVAADGRSLAHALRVLGKVEPVFVEDVAEMPEIIR
                     SVAKDGDVVVTMGAGSISAVPGKLAGKV"
BASE COUNT         3161 a         6098 c         5917 g         3350 t
ORIGIN      
        1 ttgcggccag acgcgcctgg tcggcgccgg cggagaggaa tttgtgcgga ttctggggaa
       61 cgcccttgac cagcacttcg aagtgcagat gggcgccggt ggagcgcccc gtggaaccga
      121 catcggccac acgctggccg cgacggacga tgtcgccgac acgtgcatgc agacgggatg
      181 catgcgcata gcgggtgaca atgtcattgc cgtggtcgat gtcgagcatg ttgccgtact
      241 gggggtggta gtgggcggcg atcacgaccc ctccggcggc ggcgacgatg ggcgtgcctg
      301 ccggcgccgc gaaatcgatg ccttcgtgac gcgcgctgcg gccggtgaac gggtcgatac
      361 gccagccgaa gccggaagca ttgtaggcaa cgttgaccgg atgaacggtc ggcagcagct
      421 tcgagcggat cttgtatccc atcagcgtcg attcgaccac gttcatgtag tcggcccgat
      481 actcgacgtc gcgcgcaagc gcatcgagcg actgctggaa ctcgctcatg ctcagcggct
      541 cgccgacgct cggatgggcg ccgccccggg gcggcggttc gcgaaaattg aactcctggg
      601 gctttacgcc ggccaaaccc tgaacgcgct ctccgagcgc atccaggcgc atcagctgcg
      661 cctgcatctc gccaatgcgg atcgccatcg cggcgaggtt ttccttgatg tactggtcct
      721 gcttggcggg atcgccgacg gcggcattgg ccagcgcgtc gcgcacgacg ggaatatcga
      781 gctgggcggc gtagcgcagc gtgagggcgt agagtagcga tgcggcgccg agcaccagga
      841 cgaggaaacc ggcagcggcg gctgtcaggt gccagcgggt aagcgtgacc gtcttggcgc
      901 tgaaacggtg gtgcaacagt atgacctgca tctaactcct ccgatacgtc cggtccggct
      961 gagctgtgtt aaggtgcgcg ccatgcgcaa ttcctcgttt cgctcaacgt cttccctggt
     1021 tcgcggtgcg cgcgccaata cgcgcggaac ccgggaggcg gcggattttc tccgttctca
     1081 tgaccggatg gcggccctgg tatccgtggc cgagcgcctg gcggcgctcg atcgggactg
     1141 cgcgtctctt ctgcctacgg gtttcagtgc ctgtcgcgtc ctgcacctgg acgggggtca
     1201 actggtgctt gccgttcccg gcgcggcctg ggccgccaaa ctgaagcaac agctaccgag
     1261 actgcaggac gggcttgtaa aacgtggttg gcaggttaac gcaatacgtt taaaagtgca
     1321 aggcggaaac atttccttac caacaagacc gccaaagcag ctggtgatgc cggcgacagc
     1381 ccggcaagca ttccaggagc tggaaagctc gcttgacaaa tccaagggga acgaagcgct
     1441 gaagggcgcc ttgcgggcac tgctggagcg gcacgggccg ggcggctcga accgttgatc
     1501 cggtcgggcg ccggaccgga tcaggcgtag gcccactcct cttctgcctg ctgcgcgtag
     1561 gtgcgcggcg cctgctcgat cgaatcgaag gacacgattt cgtaggcatc cggttgcgcg
     1621 agcagcgcac gcagaagctg gttgttcatt gcgtgtcccg acttgtgggc cacatagctc
     1681 gccagcaagg gatggccaac caggtaaagg tcgccgatcg cgtcgagaat cttgtgccgc
     1741 acgaattcgt tcgcgtagcg cagcccgtcc gggttgagca cgcgatactc atccatcacg
     1801 attgcgttct ccagagagcc tccgcgcgcc agccccatcc cgcgcagggt ttccacgtcc
     1861 tgcatgaagc cgaaggtgcg tgcccgcgcg atctccttca cgtaggactc cacctcgaaa
     1921 tcgacttcgg caacctgtcc ggtggcatcg acggcgggat gattgaactc gatgaagaag
     1981 cgcaggcgga aaccgttgta aggctccagg cgcgcccatt tttcctgggc gccgctgccc
     2041 tcgcgcacct cgacgggccg cttgacgcgg atgaagcgct tgggcgccgc ctgttcgagc
     2101 aggccggctt gctgcagcag gaacacgaag gtcgcggccg aaccgtccat gatcgggatt
     2161 tcctcggcgg tgacgtcgac atggagattg tcgatgccga gaccggcgca ggccgacatc
     2221 agatgctcga ccgtcgacac gcgcgcgccg tccttttgca gcactgatgc catgcgcgtg
     2281 tcgcccacgc ccattgcact ggccgggagt tcgacgacag gatcgagatc gacgcgccga
     2341 aacacgatgc ccgtgtcggg cggcgccggc cgcagggtca gttcgacctt ggtgccggag
     2401 tgcagcccga ccccgatcgt cttgatttgt tgtttgacag tgcgttgttt gagcatggcg
     2461 cgagtatagc aacttacccg cgagtcattt gtgtgcgtgt gtatcgctgt cggttcgata
     2521 ccacgccaca tttcctactc gacccaggcc cgcgcgcgct cgactgcacg acgccatgtg
     2581 gccaggcgct gggcccgctc gtcgatcgac atcgccggct cgaagcgcct ctcgacttgc
     2641 cattgcgcag cgatctcatc ctgcgaactc cagaatccga ccgcaagccc ggcgagatag
     2701 gccgcgccga gggccgtcgt ttctgtcacg gccggccgca ccaccggcac ccccagcaca
     2761 tcggcctgga actgcatcag cagatcgttg cgcgcggcgc cgccgtcaac ccgcagctcg
     2821 gtcatttcca tcgcggcgtc cttctgcatc gcttcgagta catccgcgct ctggtaggcg
     2881 atgccggcca gcgtggcgcg cgcgatgtgg gcgcgcgtgg tgccgcggct gatgccaacc
     2941 agggtgccgc gcgcatacgc atcccagtac ggcgcgccca gccctgaaaa agccggcacg
     3001 aaaaacaccc cgccggtgtc cggcacgctg gtggcaagtg cctcgacctc cgccgattcc
     3061 cggatgatgc cgagcccgtc gcgcagccat tgcacggcgg cgccggcatt gaacaccgcg
     3121 ccctccagca tgtattcggt gaccccgttt acggtccagc ctattgtggt caggaggttg
     3181 ttgcgggatg acatcgcccg cttgccggtg ttgagcagca tgaagcagcc ggtgccgtag
     3241 gtattcttgg ccatcccgca gccatggcac gcctggccga aggtggccgc ctgctggtcg
     3301 ccggcaatcc cggcgatggg tatcgccgcg ccgaagcacg ccgcttcggc atgcccgacc
     3361 acgccgctgc tgggcacgac ttccggcagc aggtccgccg gcacatcgag caattcgagc
     3421 agctcctcgt cccagcgcag ggtgtggatg ttgaacatca gcgtgcgcga ggcgttgctg
     3481 gtgtcgatca catgccggcc gcagagcttg aagatcagcc aggagtctac ggttccgaaa
     3541 gccagttcgc cgcggcgcgc gcgctcgcgc gcacccggga catggtcgag cagccacttg
     3601 agcttggttc cggagaaata ggcgtcgacc accagtccgg tcttctcgcg aaacaagggc
     3661 tcgtggccgt cggaccggag gctgtcgcac agctccgcgg tgcggtgatc ctgccagacg
     3721 atcgcgttgg caaccggttc gccggtcttg cgatcccaca gcaccacggt ctcgcgctgg
     3781 ttcgtgatcc cgatggccgc gatgctggcc gccgtgacgc cggtttcctt catcacttgc
     3841 cgggcgacgg cgagttggct gctccagatc tcgttcgcgt catgctcgac ccagcccgac
     3901 tggggaaaaa tctgccggaa ctcctgctga gcgatcccgt gcatacgccc cgcgcgatcg
     3961 aataggatgg cgcgggaact ggtggtgccc tgatcgaggg cgaggataaa gctgctcatg
     4021 accggtcgtc tcctgcttgt atcgttggtt cggccattat gaagcgactt gtcggcggcg
     4081 ccacgcgtgg cgcgccaaca aagtcgcctg gttagaaaag gcaacggcga gcctgcttat
     4141 gcgaaggctc gccgtggagc ggatcagatg ctccgctccg aggcgggaat taacgctccc
     4201 gcccttcctt ccatgcctgc agcaggacat cgtaacggat cgtctggccc ttcggcttct
     4261 cgttgtcgag cttcttccat ggcgcgccgg tatccgatag ccacttcgcc ggatcgctct
     4321 tcggattgag cttgggcgcg cagttcttca tgccgctgcg ttgcaggcgc gccatcacct
     4381 gatccatttc ctcggcaagc gtgtccatcg cctgctgcgg cgtgcgctcg ccggtcacgg
     4441 cgttggcgac gttcttccac cacagttggg ccaggcgcgg atagtccggc acgttgttgc
     4501 cggtcggcgt ccaggccacg cgtgccgggc tgcggtagaa ctcgaccagg ccgccgtact
     4561 tgtgcgcgtt gtcggtgaag tattgatggt tgatgtccga gtcgcgcacg aaggtgaggc
     4621 cgacgatgga cttcttcagc gacgtggtct tcgcgttgac gaactgcgca taaagccagg
     4681 ccgcggcctt gcggtcgtcc ggggtggacc tgaagaaggt ccaggcgccg acgtcctggt
     4741 agccgttctg catcccctgc ttccagtaag gaccgtgcgg cccgggggcc atgcgccact
     4801 tcggacggcc gtccggatgc acgaccggca ggccctgctt ggtcatgctg gcggtgaatg
     4861 cggtgtacca gaagatctgc tgggcgatct ggccctgcgc cggcaccggc cccgcttccg
     4921 agaaggtcat gccgatcgcc tcgggcggcg catacttctt catccagtcg acatacttgg
     4981 tcagcgcata aaccgccgcc ggcgagttag tcgcaccgcc gcgcgccacc gaggcgccga
     5041 ccggcgtgca cttgtcgtcg gcgacacgaa tgccccattc gtcgaccggc agtccgttcg
     5101 gaatgccctt gtcggcggcg cccgccatcg acagccaggc atcggtgaag cgccagccga
     5161 gcgacggatc cttcttgcca tagtccatgt ggccgtagat gcgccggccg ttgatctcct
     5221 tcacatcgtt ggtgaagaaa tcggcgatgt cctcataggc cgaccagttc agcggcacgc
     5281 cgagttcata cccgtacttg gccttgaagc ggtcctgcaa caccttgtcg gcgaacaggt
     5341 cggcgcggaa ccagtacaga ttggcgaact gctggtccgg caactggtac accttgccgt
     5401 ccggggcggt ggtgaaggac aggccgatga agtccttcag gtccaggtta ggattggtga
     5461 attccttgcc ctttcccgcg atgtagtccg acagcggctc gatcgcgccg tagcggtaat
     5521 gtgtgccgat caggtcggaa tcggagatcc agccgtcgta gatgctcttg ccagactgca
     5581 tcgaggtctg cagcttctcg accacgtcgc cttcctggat caggtcgtgc ttgacccgga
     5641 tgccggtgat ttcctcgaag gccttggcca gcgtcttcga ttcatagacg tgggtatcga
     5701 tggtttccga caccacgttg atttccttca cgccgcgcgc gcgcagcttc gcggcggcgt
     5761 cgatgaacca tttcatttcc tccagctgct gtgcgcgatt gagcgtcgag ggctggaatt
     5821 cctggtccac ccaacgttca gccgccttca tgtccgccca gcttgcgccg caagccagca
     5881 tggacgccat tgcaagtgcc gtcagtttca gtctcatatc tcccaccttc cgtgtatgcc
     5941 ttccccgttc ttgaaggtcg cccggcacca gggaaggctt gtgccggccg ccgcaacgct
     6001 ggttaacccc aacgcatcac gatccagagc accccgaggc ccaggaccgc gccgacccat
     6061 tgcgtcaggt cggtcaggcc agtccatgcc aggttcacat aggcggcgct gagcaggccg
     6121 atgaacagcc ggtcgccgcg ggtggtcgga atcgggagaa agcccttgcg catgatggtc
     6181 ggcgaccgca gttcgagcac ggtcatcgcg atgagcatca gcacgatggc tgtaaagaag
     6241 atggcaaccg gcgtggtcca ggccatccag gcgaatgctt cctgcatctc acaccctccc
     6301 catggcgaag cccttggcga tgtaatggcg cacgaaccag atcaccagcg cgcccggcac
     6361 gatggtcagc acgccggcgg cggccagcac gccccagtcc atgccggcgg cagagacggt
     6421 gcgcgtcatc gtcgcggcga tcggcttggc gttgaccgag gtgagcgttc gcgccagcag
     6481 cagttcgacc cagctgaaca tgaagcagaa gaacatcgtc acgccgacac ccgccttgat
     6541 catcggcagg aagatcttca cgaagaaggc cgggaagctg tagccgtcga tataggccgt
     6601 ctcgtcgatc tccttcggca cgccggacat gaagccttcc aggatccata ccgccagcgg
     6661 cacgttgaac accatgtgcg cgagcgcgac tgcgacgtgg gtatccatca gcccgacggt
     6721 cgagtacagc tggaagaacg gcagcaggaa caccgccggc ggcgtcatgc ggttggtcag
     6781 cagccagaag aacaggtgct tgtcgccgag gaagcggtag cgcgagaacg cataggccgc
     6841 cggcagcgcg atggccaccg acaacaccat gttgatgagg acgtagatga tggagttgat
     6901 gtagccgtcg taccaggatg gatcggtgaa gatggtcctg aaattgtcaa aggtcaggtt
     6961 ctgcggccac agcgtcaagg tgccaacgat ttcggcgttg gtcttcaacg acatgttcag
     7021 catccagtag atcggcagca gcgacagcag caggtagagc gccagcagcg gccgggtaat
     7081 gtgcttgctc atcatttggc gtctcccgtt ccgacacgtt gcatccagtt gtagagcacg
     7141 aagcagaaca gcaggatgat caggaaatag atcagcgaga aggctgcggc cggacccaga
     7201 tcgaactggc ccaccgcctt cgtcgtcagg tattgcgaca ggaaggtcgt cgtgctgccc
     7261 gggccgccgc cggtaagcac gaatggctcg gtgtagatca tgaagctgtc catgaaacgc
     7321 agcagcacgg caatcatcag cacgttgcgc agcttgggca gctggatgaa gcggaatacc
     7381 gcccagcgcg aggcgccgtc gatcttcgcg gcctggtagt aggcatccgg gatcgctcgc
     7441 aggccggcgt agcagagcag caccaccagc ggcgtccagt gccagacgtc catcgcgagc
     7501 acggtgaccc aggcgtcgat cggattggcg ctgtagttgt acgagaagcc gatctgattg
     7561 atcgtgtagc cgagcagccc gatgtcgccg cggccgaaaa tctgccagat cgtgccgacg
     7621 acgttccatg gaatcagaag cggaagcgca atcagcacca gcgcgagcga agcccgccag
     7681 ccctgcgcgg gcagcgtcag ggcgatcagg atgccgagcg ggatctcgat ggccagcacc
     7741 gacagcgaga acaggatctg ccgcaggagc gcgccatgca gctcgctgtc gcgcatgacc
     7801 atctggaacc actcggtgcc gacgaacacg ctctggccgg ggccgaggat gtcctggacc
     7861 gagtagttca cgatggtcat caacggcagg atcgcggaga acgcgacagc caggatcacc
     7921 ggcagaatca ggaaccaggc gcgattgttg taaggtttca tgtcaggcca cccgcttgtc
     7981 gttgacgtaa tacagcgtgc gttccggcgg caggcgcagc cacaccgtct cgcccggcac
     8041 cgcatgcatc gtgcgcagct tggccgccac gctgcgcgcc cccacctgga actcggcgag
     8101 gaaatgcgtg ccgaggtcgc gcaccgtgct gacggttgcc tgcgccgcgc cctgccccgg
     8161 cgcactcgcg cattccacga actctggccg gatgccgagc gtcgcctgcg ccgcgccctt
     8221 gagcgcggcg cgcgccttgt cggacaaggc aatgccgttg ccctccacat aggccgcgcc
     8281 gtcgcgcacg gtgcacggca ggaggttcat gccggggttg ccgatgaagt agccgacgaa
     8341 ggtgtgcgcc ggttcttcga acagttcctg cgcgctcccc gcctgcacga cctcgccctg
     8401 ggtcatcacg acgaccttgt cggcgaaggt cagtgcctcg acctggtcgt gggtcacata
     8461 gatcagcgac agcttgagtt cgtgatggat ctccttgagc ttgcgacgca gcagccactt
     8521 caggtgcgga tcgatgacgg tcagcggttc gtcgaacagc acggccgcca catccggccg
     8581 caccaggccg cggccgagcg atatcttctg cttcgcatcg gcagacagcc cgctggcgcg
     8641 gtgcttgagg tcgctggtga ggtccagcat ttcagcgacc tggctgaccc ggcgaccgac
     8701 ttcggccgcg ccccagccgc gattgcgcag cgggaaggcg agattgtcga acaccgtcat
     8761 cgtgtcgtag atgaccggga actggaacac ctgtgcgatg ttgcgctgtt cgggcggcag
     8821 cgcggtcacg tccttgccgt cgaacagcac gcgtccgtgc gagggccgca caaggccgga
     8881 gatgatgttg agcagcgtgg tcttgccgca gccggacggg ccgagcagcg cgtaggcgcc
     8941 gccgtcatcc caggtcatcg tcatcggccg cagcgcgtag tcgcccggct gcgtcggatt
     9001 gggcttgtag gcgtgcgcca gttcgaccag ttctatgcgc gccatgcttc gcctccttgc
     9061 tggtactgat tgggcgcgta cagcagttcg ccgtcgcgcg cgaaaacgaa caggtcgtcg
     9121 ggctggcagt gcaggctgca gggcaagccg atctccaggt tgtgcacgcc ttgcagctgg
     9181 gcgaccagtt cgatgcggcc gcggtgcacg tgcacatagg tctccgaacc gctgatctcg
     9241 gccagctcga tctcggcatc gatggtggcg tcgcccggcc gctgcgtcag gcgcaggctg
     9301 tgcgcgcgca agccgaggac gatgtcgcgg gcgccggccg cggcgcgctt ctggtgtgga
     9361 ttgagcggca gcagcaggcc gttgccgaga ttggcgcggc cgtcgtcctg caggaccgcc
     9421 ggcatcaggt tgatcggcgg gtcgctgaag gtgcgcgcgg cctggatgca gttcggcgcg
     9481 cggaacacgt ccagcgtcgg cccgctctgc agcagccgcc cttcatgcag cacgcaaacc
     9541 tgcccgccga gctgcagcgc ttcgagcggc tcggtcgttg catagacgac ggtggtcttg
     9601 ccgtccgcaa acaggtcgct cagctcacga cgcagttcct cgcgcagctt gtaatcgagg
     9661 ttgaccagcg gctcgtccag cagcagcagg cccgcttcct tgaccagtgc gcgcgcgagg
     9721 gcggtgcgct gctgctggcc gcccgacagc tcggccggag aacgctgcag atacggcgtg
     9781 atgtggagac gctcggccat ctcggtcacg cgcttcctga cctggtcttc gggcaggcgg
     9841 cgcagccgca gcggcgaggc gatgttctcg tagacggtga gcgacggata gttgatgaat
     9901 tgctggtaga ccatcgcgag attgcgctcg cgcaccggca cgccggtgac gtcgcgcccc
     9961 tcctccagca cgcggccgcc ggtcgggcgg tcgagcccgg ccatcaggcg catcaggctg
    10021 gtcttgccgg cgagcgtcgt tcccagcagg acgttgatcg tgccgggagc gagttgcagc
    10081 gacagcggat agacatgatc ttccgcgccg tgcttcttgc tcacgttctc aagtgtcagt
    10141 tgcactactc ctcctagttg ttcaagcgcc gcttagaagc cccaacaaga agccgctggc
    10201 agcgttgcgc ctccttgccg tactacacgt actgtctgcg tcggcgcgcc ttgccaccgg
    10261 cttcttgtta gggcttctta tgcctgcgtg cgtcttcttg tcactcgttg cgccagcctc
    10321 gcgtcgagcg ccgcttcggc acccgggggc gcgcgcaggc caagcttggt gcggcgccat
    10381 agaatgtcgg ccgcggtccg cgcccactca tgctcgacga ggtagtcgat ttccgcttcg
    10441 tacaggccgg acgtcacttc gtcacccatg tcggcgatgc agttgcgccc ggccagcaac
    10501 agctcggtgc gggtgccgta tgcccgcgcg tagcgcgcca tcagcacggg cggcagccag
    10561 gcgtagcgtt gctgcagcgc ctgcacgaag ctgtcgaact cgagcaccgc gcgcttgcag
    10621 ggcgcgctgc cgtacagatc gccgcccggc aggcaggcgt cggcggtcca ccgttcgccg
    10681 cgggcggaca gcagcggcgc gatcaggtcg accgcttcct cggcgagctt gcggaacgtg
    10741 gtgatcttgc cgccaaggac ggacagcagc ggcgctgtgc cggcttcgag ctcgagcttg
    10801 tagtcgcgcg tgacggacga cgcgtcggac gcctcgtcct cgaccagcgg gcgcaccccc
    10861 gagtacgacc acacgacgtc ggccggcgtg accggccggc tgaagtagcg tgccgacagg
    10921 tcgcacagat aggtgatttc ctcgcttgag atcgcaaccg tgccggcatc gccgtggtat
    10981 tcgatatcgg tcgtgccgat cagcgtaaac tcgccctgga acggaatcgc gaacacgatg
    11041 cgcccgtccg gatgctggaa gatgtacgca tacggatgat cgaacatgcg cggcaccacg
    11101 atgtggctgc ccttgatcag gcgcagcggc cgcgcggcgc cgcgcttgct ggccagttcg
    11161 agcacctggg cagcccaggg accggccgca ttgaccagtg cgcgagcgct caccgaccat
    11221 gcggcgccgt cctgccccca cagcgtcgcg gaccagcggc tgccgacgcg ctcgactgcc
    11281 gtgcaggcgg tacgcgtgag gatgcgcgcg ccctttgcgg cggcgtcgac tgcgctgagc
    11341 gccaccagcc gggcgtcgtc gacccagccg tcggaataga cgaaaccgcg cgtgaactcg
    11401 gccttgagcg gatctccggc gggatggctg cgcaggtcga cgctctgcga tccgggcagc
    11461 aggtcgcggc gcgcgagcga gtcgtagaga aacaggccgg tgcggatcat ccaggccggg
    11521 cgctggccgc gatcgtgcgg cagcacgaag cgcatcggcc acatgatgtg cggggctgcc
    11581 ctgagcagca cttcgcgctc gatcaaggcc ttgcgcacca gcccgaactc gcggttctcc
    11641 agataacgca ggccaccgtg gatcagcttg gatgatgcgg acgaagtgtg cgcgccgagg
    11701 tcatccttct cgcacagcac caccgacagc ccgcggccgg cggcgtcgcg cgcgatgccc
    11761 acgccattga tgccgccgcc gatgatgagc agatcgcatt cgagtagctg gcgcggcatg
    11821 tccatggctt agcgggagaa ggatgaaagg gatgaaaatg atgaaaaaaa ccttgtcatg
    11881 tacatacaat acgccaagct ccgaaaattt tgcaacgcct tttttaatct tttattttcg
    11941 tttgcgttcg ttttggagtt atattgcatg cgagaaatga ttttcgaggg gaaggaatga
    12001 cgctcaatcc acgccagcga cagttgctgg acatggtgcg caggaagggc acgatctcgg
    12061 tcgaggaatt ggctcaacag ctcgaagtga cgccgcagac cgtgcgccgc gacatcaagc
    12121 agatggagga agcgaagctg ctggcgcgct accacggcgg cgtgggcgtg ccttcttcgg
    12181 tggagaacat cgactacggc cagcgccagg cgctcaacat cgaagccaag cggcgcatcg
    12241 ccgccgcagt ggcggcgcag gtgccggccg ggtgctcgct gctgatcaac atcggcacga
    12301 cgacggagga agtggcgcgc gcactcgtcc accacgaggg cttgcacgtg gtgacgaaca
    12361 acctgaacgt ggcggcaatc ctggcggaca acccgcacag cgaagtcatc attgccggcg
    12421 gcgtggtgcg tgcgcgcgac cgcggcatcg tcggcgaggc gaccatcgac ttcatccgtc
    12481 agttcaaggt agacatcggc atcatcggga tctcgagcat cgagcccgac ggcacgctgc
    12541 gcgactatga tccgcgcgaa gtgaaggtgg cgcagaccat catcgagcag tcacgcgagg
    12601 tgtggctggt cgccgatcac gacaagttcg gccgcccggc gctggtgcgc ctggcccatg
    12661 tttcgcagat cgacatgttg tttaccgatg ccaggccgcc ggaggcgctg gcgcgggcgc
    12721 tgaaggaagc acgggtgaag gtggtggtgg cgccgtagcg ggcagccgga aggattccga
    12781 cagacatcag gaccgggagc agagcccggt cccggataca caattcttca gacctgcgcc
    12841 agcatggttt cggcgtttga cacctcgaac ttgcccggcg cctcaatgtt cacctgcttc
    12901 acgacaccat cctcgaccag catggagtag cgctgcgagc gcacgcccat gcccttggcg
    12961 gtcaggtcca gttccagtcc caccgctttc gtgaactcgc cgctgccgtc ggccatcatg
    13021 cgtaccttgc cgccggcctt ctgctcgcgg caccaggccc ccatcacgaa cgcgtcattg
    13081 accgagatgc accagatctc gtccacgcct ttgttcttca gcgcatcggc gtgctcgaca
    13141 tagccgggca catgcttggc ggaacaggtc ggggtatagg cgccgggcag cgcgaagatc
    13201 gcgatcttct tgcccttgac caggtcgctg accttgaatg cgttcgggcc gagggtgcag
    13261 ccttcggttt cggtatcgat gaattcgtac aaggtggctt caggcaggcg gtcgccgatt
    13321 ttgatcgtca tgatggtgtc cttgttctga tgggaaatca cgcgctgcgg ccgccgtccc
    13381 gcgcatgcgg aagcggcgac tgcggcacaa gtagaaacgc cgcgccgggg cgagccctcg
    13441 gcgcggcgct cagagcgcag tatgccggcg aattgcgatt tctgcagtgg cggccggctg
    13501 gcgcgcgtca gtccgcctgc ttgcgcagga aggccgggat gtcgtaggtc tccatcccgt
    13561 tcttctcgag cgcgcgcacg gtttccgacg ccgattcgcg acgccacacc gccggcgcct
    13621 tcaggccgcc gaagctggcg ccgacggcgc ccgtggcggt ctgggcgacg gtggcgtcgg
    13681 ccatgatcgg ctcgttgtgc gtgccggtgc gcaacaccgg ggtctgcacc agctgcacgt
    13741 tcttgcgggc gcggcccagg ccagttgcaa ccacggtcac gcggatgtcg tcgcccatcg
    13801 aatcgtcgta ggcaatgccc tgcgcgatcg atgcgtccgg agcggcgaag gcgcgcacgg
    13861 tggccatcac ttccttgatc tccttgccct tcaggctgcg gctcgcggtc acattgacca
    13921 gcacgccgcg cgcgccggac aggtcgatgc cgtccaagag cggcgaagcg acggcctgct
    13981 cggccgcgat gcgcgcacgg tcgacgccgg aagccgtcgc ggtacccatc atcgccttgc
    14041 cctgctcgcc catgatggtc ttgacgtcgt tgaagtcgac attgatgtgg ccgggcacgt
    14101 tgatgatctc ggcgatgccg gcgaccgcat tgttcagcac gtcatcggca tgctgcagcc
    14161 actcgatcat gctgtcgtcc tcgtagatgt cctcgagctt ctcgttgagg atcacgatca
    14221 gcgaatcaac atgctgggac aaggcttcca gaccctcgtc ggcgatttcc atgcacttgg
    14281 cgccttcgta ggaaaagggc ttcgaaacga ccgccacagt caatgcaccg agttccttgg
    14341 cgacctgggc gacgataggc gcggcgccag tgccggtgcc gccgcccatg ccggcggcga
    14401 tgaacaccat gtgcgcgccg cgcagtgcgt cctcgatgcg cgcgcgcgat tcctcggcca
    14461 gcttgcggcc gacctccggc ttcatgccgg cgccaagacc cgtgtcgccg atctggatca
    14521 cattgtgcgc cttcgactgc gcgagcgcct gggcgtcggt gttggcagcg atgaattcca
    14581 cgccggacac gcccttgttg atcatgtgct gcacggcatt gccgcctgca ccgcccacac
    14641 ctaccacctt gatcacggtg ccgagatttg cgttatcgat catgtcgatt tccatggttg
    14701 actcccttat caatgatgca gttgtcagtc gttagtcact acgcgatgtg gcgactagcc
    14761 actgctaact gcggttaatt acagatgccc aaaacttaaa acgtcccgaa gaaccactcc
    14821 ttcatgcgct tccataccgc ccccaccgaa ccgtcctggc gcgtcaccag atgcccgcgc
    14881 aggtattgct tctttgcttc catcaacagg ccgagcaccg tggaatagcg cggactgcgc
    14941 accacgtccg ccagctggcc gctgtattcc gggacaccca ggcgggtcgg cttgaggaaa
    15001 atgtcttcgg ccagctcgac cattcccggc atcgtcgacg aaccgccggt cagcacgatg
    15061 ccggaagaca gcacttcctc atagccggat tcgcgcacga cctggtgcac cagcgcgaac
    15121 agttcttcga cccgcggctc gatcaccgcc gccagggcct gacgggacag cgcacgcgga
    15181 ccgcggtcgc ccaggcccgg cacttccagc gtttcgttcg gatcggccag cacctgcttg
    15241 gccacgccga aacgcagctt gatctcctcc gcctcggcgg tcggcgtgcg cagcgccatc
    15301 gcgatgtcgt tcgtaatctg gtcgccggca atcgggatca ccgcggtatg gcggatcgcg
    15361 ccttcggtga agatcgccac gtcggtggtg ccgccgccga tgtcgaccag caccacgccg
    15421 agctccttct catccggcgt cagcaccgcg tcggcggagg ccatcggctg caggatcaga
    15481 tccgagactt caagcccgca gcggcgcacg cacttgacga tgttctgtac ggccgacacc
    15541 gcgccggtga cgatgtgcac cttcacttcc aagcggatgc cgctcatccc gatcggctcg
    15601 cgcacgtctt cctggttgtc gacgatgaat tcctgcggga ccgtgtgcag cagctgctgg
    15661 tcggtcggga tgttgaccgc cttggcggtc tcgatcacgc gcgccacgtc ggtcggcgtg
    15721 acttccttgt ccttgatcgc caccatgccg ctggaattga agctgcggat atggctgccg
    15781 gcgatcccgg tgtagacatt gcggatcttg cagtcggcca tcagctcggc ttcctcgagc
    15841 gcgcgctgta tcgattccac cgtcgcctcg atgttgacca ccactccctt cttcaagccc
    15901 ttcgactcgt gctggccaag gccgatgact tcgtgcctgc cgttgggcag cacctcggcc
    15961 acgaccgcca ccaccttcga ggtgccgatg tcgaggccga cgatcaggtt cttcgcgtct
    16021 tttgtcatga tgatccgctt attttttctt cccttcggta gccagcttca ggccctgcgc
    16081 gctcaatgcc agcccgttcg gataccgcat atcgactgtt tcaatcctgt cctgcaggcg
    16141 cgcggccagc tgcgggtaaa tcccgaccag ccgctgcgcc ctttcctgca ccgaaacgcg
    16201 cgcctgctcg cgtccgagtt ccaccgtcat gccgccgacc agcttcaccg tccacgcata
    16261 gcgctcggac agggcaaccg attccggcac cagccccgcg ggcttgagcc attcatgcag
    16321 ctcgcgaaag cgcgccacca cgtccttcgc actgccctcc ggtccggaga acaccggcag
    16381 cggcccgtcc gcctccgctt cggccaggtt cgcggtgaat acttccccct tcaccgacag
    16441 caacctgccg tcgtcgcccc acgcgcccag cgcgcggtgc tcctccacgt tcacgatcag
    16501 ccggttcggc cattcgcgcc gcactgtcgc ctggcgcacc cacggcacgc tctcgaacgc
    16561 ctggcgcacc gcatcgaggt cggcggtgaa gaaattcccc ttcacgcccg gcagcgccgt
    16621 cgtgcggatc gtcggcgcgc tcacatgccg cagcggcgca ccttgcgcac cttcaacgac
    16681 gaccacccgg aaggtgaaca tcggtcggtg cgcaacccac cagaagcccg acgcgagcaa
    16741 tcccagcacc agcagtccga gcagtgcatt ggttgtcgca ttgagcgtcc gtacgtcgtg
    16801 ccacatcgcg tcaggccggc cgccactccg tattcggttt caactccagc gtcgccatgc
    16861 gcaggatttc cacgcacagg tcctcgtagc tcacgccgtc ggccttggcc gacatcggca
    16921 ccagcgaatg tccggtcatc ccgggagagg tattgatctc cagcaggaag ggctcgttgt
    16981 ccgtcgcgcg cagcatgaaa tccacgcgcg cccagccggc gcagccgagc gcattgaacg
    17041 cgcccacggc aagcgactgg atgcgcctgg tcaatgcctc gtccagcggc gccgggcaca
    17101 ggtactgcgt gtcgtcgctg aaatacttgt gttcgtagtc gtagttgccc tgcggggcgc
    17161 ggatctcgac gatgggcagc gcgcgcgcat ggcggccgcg cccgagcacc ggcacggtca
    17221 gttcgcgtcc gctgatgaat tcctcggcca gcacgacttc gtcatacttc gcgcacagct
    17281 cgaacccgtt ctgcatctcc gaatagccgg cgaccttggc gatgccgatc gtcgaaccct
    17341 cgtgcggcgc cttcagcatc agcggcaggc cgagttcgtc cggcaccgcg cgcaactgct
    17401 cgcgcgtggt gtcgcgtgca tcgagcacga cgaagcgcgg cgtcggcagg ccgtggttga
    17461 tccagatgcg cttgctcatc accttgtcca tgccgatcgc cgaagccatc acaccggatc
    17521 cggtgtacgg aatcccgagc tgctccagaa cgccctgcag cgtgccgtcc tcgccgtagc
    17581 ggccgtgcag cgcaatgaac gcgcgatcga acttctgcgc cgccagctcg gcgatgctgc
    17641 gctcaccggt atcgaaaccg tgcgcgtcga tgcccttgct ctgcagcgcc ttcagcacgc
    17701 cgctgcccga catcagcgag acttcccgct ccgccgagcg gccgccgtac agcacgccga
    17761 cctttccgaa ttccttgccc attgcctaca ccttccccgc cagcttcccc ggcactgcac
    17821 tgatcgatcc cgcgcccatc gtgaccacga cgtcgccatc ctttgcgaca ctcctgatga
    17881 tctccggcat ttccgccaca tcctcgacga acaccggctc caccttgccc agcacgcgca
    17941 acgcatgcgc gagagagcgg ccgtcggcgg cgacgatcgg cgcttcgccg gccgcgtaca
    18001 cttcgccgag caccagcgca tcgacgctcg acatcacctt cacgaagtcc tcgaacaggt
    18061 cgcgggtgcg ggtgtagcga tgcggctgga aggccagcac cagacggcgg ccgggatagg
    18121 cgccccgtgc ggccgcgatg gtcgccgcca tctcgaccgg gtgatgacca tagtcgtcga
    18181 ccagcgtgaa cgcgccgccg cccggcaagg ccacgtcgcc gtagcgcgtg aaacggcgtc
    18241 caacgccgtt gaattccttc aacgccttct gcgtcgcgct gtcatcgaca ccgagttcgc
    18301 gcgcgatcgc gatcgccgcg caggcgttct gcacgttgtg catgccgggc tggttcaggt
    18361 gcacctgcag cggcggatag tccttctgca gcacggtgaa ttccatgtgg ctgtcgaccg
    18421 ccttcgcatc caccgcgcgc acctcggcct ccggatgaaa gccgtatgtg acgatgggct
    18481 tggagatgaa cggcatgatc tcgcgtacgt tcgggtcgtc caggca
//
LOCUS       DATLGF010000050         5558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_49700,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000050 DATLGF010000000
VERSION     DATLGF010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49700"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VM406_01695"
     CDS             <1..801
                     /locus_tag="VM406_01695"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HVL74702.1"
                     /translation="LGTPVIVENRPGAAGRIAAEAVKNAAPDGKTLLVTPVAMMSLFP
                     KIYKNLRYDPVNDFAPVAQLSVFEYAVAVSPQSPVKNVTELADWVRKNPQRANYGTSA
                     PGSLPHFFGVMFAKTANIEMTHVGYKGSTPALSDLIGGHLPILFNTASDLAEMHKAGR
                     IRILATSGSQRLQALPDVPTFKESGYKLEAVGWNGMYAPAGTPRDVIEKLNRAAVDAL
                     KDPDVKSKLALRGFTATGTSPQDLARIQKADAELWAPAIEASGFTPED"
     gene            830..2398
                     /locus_tag="VM406_01700"
     CDS             830..2398
                     /locus_tag="VM406_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HVL74703.1"
                     /translation="MGTPKNVLFIMCDQLRADHLSCYGHPYLHTPNIDALARRGVRFE
                     QAFVQSGVCGPSRMSFYTGRYPSSHGATWNRVPLSMNEITLGEYLRGRGMDLALAGKT
                     HVLPDKHAMERLDMDGGSALAALLREGGFSLVDRYDGHHAEPEGDYAHYLRRNGYESS
                     DPWSDFVIAAINEQGEKVSGWNMRNVHLPARVRKEHSETAYTTDQAIDFIRSRGDAPW
                     ALHLSYVKPHWPYMAPAPYHNMYTLDQCLPLRRHKDELISQHPVLEAYRQQEECANFM
                     REEVSNAVRPAYQGLIKQLDDEIGRLWETLEKLGRWDDTLVVFTADHGDYLGDHWLGE
                     KEQFHDTVQRVPYLVYDPSEKADLTRGSVSRQLVEAIDTVPTILDALECGIPAHRVEG
                     RSLLALTRASDVAGAAWRDAVFSELDYSYRLARRLLGRDVDECRAWMVRTQNWKYVHW
                     QGYAPQLFDLRTDPDEFRDLGTSAEHASVRAEMHQRLFDWFCKLKRRTTVTHEEVERG
                     TNRYKEAGVFYGVW"
     gene            complement(2487..3332)
                     /locus_tag="VM406_01705"
     CDS             complement(2487..3332)
                     /locus_tag="VM406_01705"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HVL74704.1"
                     /translation="MNEQMAAVQPAQYRPWDIGTGVHGYAWEAPRPRAAILLQHGFAE
                     HAQRYVRQYSALIPHLLDIGISVYAFDLRGHGQSPGRRAVTDVETAVADHLAARRKLE
                     ALPLPLFLIGHSLGGIVTATSVVRNPAALQGVILSSPALLVSANPLTRLFARVVGAIA
                     PSLPAKSLPPSGISRIPEQVLQLVQDPLAYHGRMPAGLAASILFTSRGNWNRYRDWQA
                     PTLVIHGTADTFTEPEGSLRFIDAIRSADKTLHLVEGGYHELLNDVGSEQVLQVILGW
                     LEERI"
     gene            3533..4897
                     /locus_tag="VM406_01710"
     CDS             3533..4897
                     /locus_tag="VM406_01710"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HVL74705.1"
                     /translation="MSDLLSKLHRNPLSRPFMRVLGLPNPVNLARAEGGYVEEPFAGR
                     TALLLPTAGGYAFGQLAQALEQAGASLRHSIDESDTAPLDIVVMDATGLRAPEDCRAL
                     YDGFQPVIRRIARNGRVLLLAALPDEAADPVAAAVARGVEGFSRSLAKELGRKGVCVN
                     LGYVSPAAVDRLDGPLRFFCGPQSTYVSGQAVRIRERVAAPAALLRTRALEGKTALVT
                     GAARGIGMAAAERLAEEGARVICLDIPTALDDLNDTCARVGAHPLALDISTSDAPSRL
                     AQALREPQWRGGVDIVVHNAGITRDRTLAKMSAREWDMVIAVNLAAIAAIDETLVSET
                     LLRDEGRIVCVSSISGVAGNFGQTNYAASKAALIGYAAAMASRLAARGICINAVAPGF
                     IETAMTGKMPFFTREIGRRLNSLQQGGQPRDVAELITFLATPGAHGISGETIRVCGQG
                     MVGA"
     gene            4935..5291
                     /locus_tag="VM406_01715"
     CDS             4935..5291
                     /locus_tag="VM406_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74706.1"
                     /translation="MQESLAVALDHGVLLAQPGTRASLQTSNARISSEGVMGDHELSY
                     PRAGSQLAFARAATALAVVSVGAFAIGALAVRWLAIKQLAIEGAAIKRLRIGELEVDR
                     LQVNETITQHQPSSPS"
BASE COUNT         1002 a         1817 c         1810 g          929 t
ORIGIN      
        1 cttggaactc cggtgattgt cgagaacagg ccgggcgccg ccgggcgtat tgccgccgaa
       61 gccgtgaaaa atgccgcgcc tgacggcaag accctgctcg tcacgccggt ggcgatgatg
      121 tccctgttcc ccaagatcta caagaacctg cgctacgatc cggtgaatga cttcgcgccc
      181 gtcgcgcagc tgtcggtttt cgagtacgcg gtggcggtgt ctccccagtc gcccgtgaag
      241 aacgtcactg aactcgccga ctgggtgcgc aagaacccgc agagagcgaa ctatgggaca
      301 tcggcaccag gctccctccc ccacttcttc ggcgtaatgt tcgccaagac cgccaacatc
      361 gagatgacgc atgtgggcta caagggctcc acgccggcgc tcagcgatct gatcggcggc
      421 caccttccga tcctcttcaa taccgcatcc gacctggccg aaatgcacaa ggccggacgt
      481 atccggatcc ttgcgacttc gggatcccag cgtcttcaag cgcttcctga tgtccctacc
      541 ttcaaggagt cgggatacaa gctcgaagcg gttggctgga acgggatgta tgcccctgcc
      601 ggcacgccgc gcgacgtgat cgaaaagctc aaccgggctg ccgtcgacgc gctcaaggat
      661 ccggacgtga aatccaagct cgcgctgcgc ggcttcaccg cgaccgggac ctcgccacag
      721 gacctggcgc gcatccagaa ggcagacgcg gaactgtggg cacctgccat cgaggcctcc
      781 ggattcacgc cggaagactg agcaatcccg tccctcacat ctttcgaaaa tgggcacgcc
      841 gaaaaacgta cttttcatca tgtgcgacca gttgcgggcg gatcatctgt cctgctacgg
      901 gcacccttac ctgcacacgc cgaacataga cgcgctggca cgccgcggcg tgcgtttcga
      961 gcaggcattc gtccagtccg gcgtatgcgg tccgtcgcgc atgtcgttct ataccggtcg
     1021 ctacccgtcc agccatggcg cgacctggaa cagggtgccg ttgtcgatga acgaaattac
     1081 gctcggcgaa tacctgcggg gccgcgggat ggacctggca ctggcaggga agacgcatgt
     1141 gttgccggac aagcacgcga tggagcggct cgacatggac ggcggctccg cactggcagc
     1201 cttgctgcgc gagggcggat tcagcctggt ggaccgctat gacggacatc atgctgagcc
     1261 cgaaggggat tacgctcact atctgcgccg caacggctat gagagcagcg atccttggag
     1321 cgacttcgtc attgccgcca tcaacgagca aggcgagaag gtatctggat ggaacatgcg
     1381 caacgtgcac ctgccggcgc gcgtacgcaa ggagcattcc gagacggcct acacgacgga
     1441 ccaggcaatc gacttcatcc gttcgcgcgg cgacgcgccc tgggcactcc acctttccta
     1501 cgtcaagccg cactggccct acatggcccc ggcgccgtac cacaacatgt acacgctgga
     1561 tcagtgcctg ccactgcgcc gccacaagga cgaattgatc agccagcatc ctgtgctgga
     1621 ggcctatcgg cagcaggagg aatgcgcaaa cttcatgcgt gaagaagtca gcaacgccgt
     1681 gcgtccggcc tatcagggcc ttatcaagca gctggacgac gagataggac gcctgtggga
     1741 aacgctggaa aagctgggcc gctgggatga cacactcgtc gtgttcaccg cggaccatgg
     1801 cgactacctc ggcgaccact ggcttggcga gaaggagcaa tttcacgaca cggtgcagcg
     1861 ggtgccgtat ctcgtctacg atccgtccga gaaggcggac ctcacccgcg gatcggtgag
     1921 ccggcaactc gtggaagcga tcgataccgt tccgaccata ctcgatgcac tggaatgcgg
     1981 gattccggcg catcgcgtcg aaggccggtc gctgctcgcc ctgacccgcg ccagtgacgt
     2041 ggccggcgcc gcgtggcgcg atgcggtgtt ctccgagctc gattactcat atcggctggc
     2101 gcgtcgcctg ctcgggcgcg acgttgacga atgccgcgcc tggatggtcc gtacccagaa
     2161 ctggaagtac gtgcattggc agggctatgc gccgcagctg ttcgatctgc gcaccgatcc
     2221 ggacgagttc cgcgacctcg ggaccagcgc ggaacatgcg tcggtgcggg cggaaatgca
     2281 tcagcgcctg ttcgactggt tctgcaagct caagcggcgt accactgtaa cgcacgaaga
     2341 agtcgaacgc ggcacgaacc ggtacaagga ggcaggcgtc ttctatggcg tgtggtgagc
     2401 ggcacctccg gtgccgtcca tgccggtgcg gcgcgactgt cagaaagcgt cgagggcgct
     2461 gatgctggtg cggtgcggcg gcgctatcag atgcgctctt ccagccagcc gaggatcacc
     2521 tgcaacacct gttcgctgcc gacatcgttc aacagctcgt ggtatccgcc ctcgaccagg
     2581 tgcaacgtct tgtccgcgga ccggatcgcg tcgatgaagc gcaggctgcc ttcgggttcg
     2641 gtgaaggtgt cggcggtgcc gtggatcaca agcgtcggcg cctgccagtc gcgataacgg
     2701 ttccagttgc cgcgcgaggt gaacaggatg ctcgccgcca gcccggccgg catgcgccca
     2761 tggtacgcga gcggatcctg cacgagctgc agcacctgct cgggaatgcg cgagatgcct
     2821 gaaggcggca gcgacttcgc gggcaaggag ggtgcgatcg cgccgaccac gcgcgcaaac
     2881 aggcgggtga gcggattggc actgacgagc agcgccggac tactgaggat cacgccctgc
     2941 agcgcggcag ggttgcgcac cacgctggtg gcggtgacga tgccgccgag cgagtgcccg
     3001 atcaggaaga gcggaagcgg cagcgcttcg agcttgcggc gtgcggcgag gtgatcggct
     3061 accgccgtct cgacgtcggt caccgcgcgc cggcccggag actgtccgtg cccgcgcaga
     3121 tcgaacgcat agacactgat cccgatatcc agaagatgcg gaatcagcgc gctgtactgc
     3181 cgcacatagc gctgcgcatg ctcggcaaac ccgtgctgca gcaggatcgc cgcgcgcggc
     3241 cgcggcgcct cccacgcata gccgtgcacg ccggtgccga tatcccaggg ccggtactga
     3301 gccggctgta cggccgccat ctgttcgttc atcgcgtctc cctcgaaaac cttcgacttg
     3361 ccgcgcatgc gtgagccgcc cgcggaccag ccgccattgt cattcacaca tgcgcggatg
     3421 gaaagcacgc ggtacgcagg attgcccgca ttgtccgcgt cacctatgat gggcgccgcc
     3481 ctccgggctg agccggcggc gcgaaatacc catatcgaaa agcgaaaaac cgatgtccga
     3541 tctgctgagc aagctgcacc gcaatcccct gtccaggccc ttcatgcgcg tgctgggtct
     3601 gccgaatccg gtgaatctcg cgcgggccga aggcggctac gtggaagagc cgttcgcggg
     3661 aaggacggca ctgctgctgc cgacggccgg cggctacgca ttcggccagc tcgcacaggc
     3721 tctggagcag gcgggcgcga gcttgcggca ttcgatcgac gaaagcgaca ctgcgccgct
     3781 cgacatcgtc gtcatggatg cgaccggcct gcgcgcaccc gaggactgcc gtgcgctgta
     3841 tgacggcttc caaccggtca tccgccgcat cgcccgcaat ggccgtgtgc tgctgcttgc
     3901 cgcgttgccg gacgaggcgg ccgatccggt cgccgcagcc gtcgcgcgcg gcgtcgaggg
     3961 attcagccgc tcgctcgcca aggagcttgg ccgcaagggc gtctgcgtga atctcggcta
     4021 tgtgtcgccc gccgccgtcg atcggctgga cggaccgctg cgctttttct gcgggccgca
     4081 atcgacctac gtgtcgggcc aggcggtgcg catccgcgag cgcgtggcgg cgcccgccgc
     4141 gcttctgcgc acgcgggctc tggagggcaa gaccgcgctc gtcaccggcg ccgcacgcgg
     4201 catcggcatg gccgcggccg aacgcctcgc cgaggaaggg gcgcgcgtga tctgcctgga
     4261 catcccgacc gcgctggacg acttgaacga cacctgtgcg cgcgtcggcg cgcatccgct
     4321 cgcgctcgac atttccacct ccgacgcgcc gtcgcggctg gcgcaagcct tgcgcgagcc
     4381 gcaatggcgt ggcggcgtag acatcgtcgt gcacaatgcc ggcatcacgc gcgaccgcac
     4441 gctggcgaag atgtccgcgc gcgagtggga catggtgatc gccgtgaacc tcgcggcgat
     4501 tgccgcaatc gatgaaaccc tggtgtcgga aacgctgctg cgcgacgagg gccgcatcgt
     4561 ctgcgtgtca tcgatcagcg gcgtggccgg caacttcggc cagaccaatt acgccgcctc
     4621 gaaggccgcg ctgatcggct acgcggccgc gatggcgtcg cgccttgccg cacgcggcat
     4681 ctgcatcaac gccgtcgcac cgggcttcat cgaaaccgcg atgaccggga agatgccgtt
     4741 cttcacacgc gagatcgggc gccggctgaa ttcgctgcaa caaggcgggc agccgcgcga
     4801 cgtggccgag ctgatcacct tcctcgcgac acctggcgcg cacggaatca gcggcgagac
     4861 gatacgtgtt tgcggacagg gaatggtggg cgcgtgaacg gcagttcctg acgatagtcg
     4921 ggcatcagag caacatgcaa gaaagtctgg cggttgcgct cgatcacggc gtcttgcttg
     4981 cgcaacccgg aacgcgcgca tctctccaaa cctctaacgc acgcatttct tcggagggcg
     5041 ttatgggcga ccacgaactt tcctatccgc gtgccggttc gcaactcgcg tttgcacgtg
     5101 cggcgactgc ccttgccgtg gtgagcgtcg gcgcgttcgc gatcggcgcg ctggcggtgc
     5161 gctggctggc gatcaagcaa ctggctatcg aaggggcggc aatcaagcga ttgcgcatcg
     5221 gtgaacttga ggttgaccgc ttgcaggtga acgagacgat cacgcagcac cagccatcgt
     5281 ctccttcatg acgtgagcac gccttggaag ccctgacaac atgccgatgg caaggcgcgc
     5341 agtcgcagac agcaggtggt gtacggcaag gaggcgcaac gccgccggcg gcattttgcc
     5401 agggcttctt accgcgaccg cccgcgttcg accggtatgc cccagatccc gatgcccttc
     5461 gcgtgtgcga tgcgttcgag cgccgtgtat tcgaaaggcg catccggtgt cgccacagcc
     5521 cagccccgtt gcagcatcca tgcgccgaga tcgtcgcc
//
LOCUS       DATLGF010000051         3133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_18599,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000051 DATLGF010000000
VERSION     DATLGF010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18599"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="VM406_01720"
     CDS             <1..1209
                     /locus_tag="VM406_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74707.1"
                     /translation="RPAGSAGVEYRAPTREELLRIEREVLTPLLGLANTSDPAGAKQA
                     PSTFAAAGASARAAGLELRWLGGSTGSYFLLSDEGNGRGWILIRAGQAENLVLAVPRP
                     QAEKGTLETALAAFEDLQARMLVIAGAEPDANRDAKTDVLAVANAASLFTLAHQAALR
                     AMDTEPGAVLHVRAFGVRADAPAATEDALLSFDDGARDRNALTGAAAKVLRGLEDMGF
                     NVRVGTGAEDSAGYDATTGPQASYMSQTRNKRFGALWISPLARQQLGEGALESGRRQF
                     AALGMLVQQGAIEQALAERRMGTRRLPAALRESAEKYRQTQDIVLLSAMQKRFADLQF
                     ERFEDSGGRGAYLLVSDGSRSLVGVLALSAPARGDAQDRTAIAVPPGPLARQDVARFI
                     RSRSQWMVIE"
     gene            1206..>3133
                     /locus_tag="VM406_01725"
     CDS             1206..>3133
                     /locus_tag="VM406_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74708.1"
                     /translation="MSLRLIFRLASLILVAAAALFGASRFQASPLPQAEVGATFRALS
                     EHAEATRGNVLTVGGPGVVYALPPSAGAVRVLSNANLFEVEHARAARQADAQRRWNYA
                     IEVQELDAGGTVLSERVHHFRRDLVEFYLPEGVRGTGSFYLEQSAPFPLAAANTRMRF
                     DAGARPARLRLRLLSADPDIADVLVRLAVPVPVSQARAESVWRRLNDAQRERLAMGNV
                     FPPKLLTEAERASLIASRWQPIGAQEPSTERDIYVLRGYDLGAPLDRVQVPGLAVGPS
                     RWATVQLPEQGGEVRIVLETDEVTGESSAQSEQVAVRWFGHSAFLRRESSHAWQGGQF
                     ERREKFPGGWLEVRASREASVRFLLVEGERETDITPPVQHLRAWEAKPADQAAHEDVR
                     DTAALEFSISHAANASTPLRLVLRRTVAAAARLSSAPVEVSFLDAEGATVRKFELKPE
                     MLASSYDAPWPEAAGEQVSDPWEVFFAVPQQVAKLRIAATAPTLVNAYVRPAHLPRML
                     RMPEDLTTPEAAETAIPGWFALNPDRHDARILAGQSSLLTLRGRPPEDRPELSAGQYQ
                     WEDFDPVQGGAARVFLAPREAGVPERREAIGQTYRPLPSDKRASFVSEPGRKQVTARL
                     AWTGAGQEAFRFKVLLPGT"
BASE COUNT          502 a         1026 c         1104 g          501 t
ORIGIN      
        1 cggccggcgg gctccgcggg cgtggaatac cgcgcaccga cccgtgaaga actgcttcgc
       61 atcgaacgtg aagtcctgac gcccttgctc ggactggcga acacatccga tccggccggc
      121 gccaagcagg cacccagcac gtttgccgcc gccggtgcga gtgcacgcgc cgcagggctc
      181 gagctgcgct ggctgggcgg cagcacggga agctacttcc tgctgtccga cgaagggaac
      241 ggacgcggct ggatcctcat ccgcgctggc caggccgaga acctggtact tgccgtgccg
      301 cgaccgcagg ccgagaaggg cacgctggaa acggcgctcg cggccttcga ggatctgcag
      361 gcacgcatgc tggtcattgc cggtgccgaa ccggatgcga atcgcgatgc aaaaaccgac
      421 gtgctggccg tcgccaatgc cgccagcctg ttcacgctgg cgcatcaggc ggcattgcgc
      481 gccatggata cggagccggg cgcggtgctg cacgtgcgcg ccttcggcgt acgcgccgat
      541 gcgccggcag cgaccgaaga tgcactgctg agcttcgacg atggtgcgcg ggacaggaat
      601 gcgctcaccg gcgcggccgc caaagtgctg cgcggcctgg aagacatggg cttcaacgtc
      661 agagtcggga ccggcgccga ggacagcgcg ggctatgacg caacgacggg cccgcaagcc
      721 tcctacatga gccagacccg gaacaagcgg ttcggtgcgc tgtggatctc gccactcgca
      781 cgccagcagc tcggtgaggg tgcattggaa agcgggcgta ggcagtttgc cgcgctgggc
      841 atgctggtac agcaaggtgc gatcgagcag gcgcttgccg agcgccggat gggcacgcgc
      901 cggcttccgg ccgcactgcg cgaaagtgcg gagaagtacc ggcagaccca ggacatcgtg
      961 ctgctctccg caatgcagaa acgctttgcc gacctgcagt tcgaacgatt cgaggacagc
     1021 ggcggacgcg gcgcctacct gctcgtcagc gatggcagcc ggtccctggt cggcgtcctg
     1081 gccctgagcg cgcctgcccg gggcgatgcg caagacagga ccgcgatcgc ggtgccgccg
     1141 ggtcctctgg cgcgacagga tgttgcgcgt tttatccgca gccgtagcca atggatggtg
     1201 attgaatgag tttgcgactg atattccgtt tggccagcct gatcctggtc gccgccgctg
     1261 ccttgtttgg cgcatcccgt tttcaggcat cgccgctgcc gcaggccgag gtcggtgcaa
     1321 cattccgcgc actgtccgaa catgccgaag ccacgcgcgg caatgtgctg acggtcggtg
     1381 gccccggcgt cgtctatgcg ctgccgccat ctgccggcgc ggtacgcgtg ctcagcaatg
     1441 ccaatctgtt cgaggtcgag catgcacgcg ctgcacggca agccgatgcg cagcggcgct
     1501 ggaactatgc gatcgaggtg caggaactgg atgcgggcgg aaccgtcctt tccgaacgcg
     1561 tgcaccactt ccgtcgcgac ctggtcgaat tctatcttcc ggaaggcgtg cggggcaccg
     1621 gctcgttcta tctcgaacag tcggcgccgt ttccgcttgc tgccgcgaac acgagaatgc
     1681 gcttcgacgc cggcgcgcgc cccgcacgcc tgcgcctgcg gctcttgagc gccgaccccg
     1741 acattgccga tgtgctggtg cggcttgcag tgccggtccc ggtatcgcaa gcgagagcgg
     1801 aatcggtatg gcgtcgcttg aatgatgcgc agcgcgagcg tttggcaatg ggtaacgtgt
     1861 ttccgcccaa gctcttgacc gaggccgagc gagccagcct gatcgcatcg cgctggcagc
     1921 cgattggcgc gcaggagccg tcaacggagc gcgacatcta cgtgttgcgc gggtatgacc
     1981 tcggtgcgcc gctggatcgt gtccaggttc cggggctggc ggtcggcccg tcgcgctggg
     2041 cgacggtgca gttgcccgag cagggcggcg aggtgcgcat cgtgctggag accgacgagg
     2101 tgacggggga atcctctgcc cagtcggagc aggttgcggt gcgttggttt ggccacagcg
     2161 cctttctgcg gcgcgagtcc agccatgcct ggcagggcgg tcagttcgag cgccgggaga
     2221 agtttcccgg cggctggctt gaagtgcgcg ccagccgcga ggccagcgtc cgcttcctgc
     2281 tggtcgaggg cgagcgcgag accgacatca cgccgccggt gcagcacctg cgcgcctggg
     2341 aggcgaaacc ggccgatcag gcggcgcacg aggacgtgcg cgacacggca gcgctcgagt
     2401 tttcgatcag ccacgcagcc aatgcgtcga cacccttgcg tctggtgttg cgccgcacgg
     2461 ttgccgcagc cgcgcggctg tcatcggctc cagtggaagt aagcttcctg gacgcggaag
     2521 gcgccacggt gcgcaagttc gagctgaagc cggagatgct ggcgtcctcc tacgacgcgc
     2581 cctggccaga agcagccggc gaacaggttt ccgatccctg ggaagtgttc ttcgcggtgc
     2641 cgcaacaggt ggcgaagctg cgcattgccg ccacggcgcc gaccctcgtc aacgcctatg
     2701 tgcgccccgc gcacctgccg cgcatgctgc gcatgccgga agacctgacc acgccggaag
     2761 cggccgagac cgcgattccc ggatggtttg cgctcaatcc ggacaggcat gacgcacgca
     2821 tcctggcagg gcagagcagc ctgctgacgc tgcgcggccg cccgccggag gacaggcccg
     2881 agctcagcgc cggccagtac cagtgggagg atttcgatcc ggttcagggc ggggccgcgc
     2941 gcgtgttcct tgcgccgcgc gaagccgggg tgccggagcg gcgcgaggcg atcggccaga
     3001 cctatcggcc gctgccatcc gacaagcgcg cctccttcgt ctccgaaccc ggccgcaagc
     3061 aggtgacggc aaggcttgca tggaccggtg ctgggcagga agccttccgc ttcaaggtct
     3121 tgctgcccgg cac
//
LOCUS       DATLGF010000052         3300 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_34172,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000052 DATLGF010000000
VERSION     DATLGF010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34172"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..540)
                     /locus_tag="VM406_01730"
     CDS             complement(34..540)
                     /locus_tag="VM406_01730"
                     /inference="COORDINATES: protein motif:HMM:NF044847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74709.1"
                     /translation="MNDVRLLVNGKEYGGWKDIEITAGIERQVRDFTLAVTDRWPGQQ
                     DLPRRIRPGDVCELFIGKDKLLTGYVDATPIRYDGKSVSVGVKGRSKTADLVDCSAVH
                     SPGRWSGARVDRIAADLARPYGIEVQAQVPVGAPMTHAIDQGESVFESIACVIGAPTG
                     TCACTSIP"
     gene            complement(537..1748)
                     /locus_tag="VM406_01735"
     CDS             complement(537..1748)
                     /locus_tag="VM406_01735"
                     /inference="COORDINATES: protein motif:HMM:NF018818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HVL74710.1"
                     /translation="MAWKDKLQPASFRGVPFEVESDDATFGRRVQVHEYPHRDQPYVE
                     DLGRAAREMNITAFLVGPDYMDKRDRLLAAIEEAGPGTLIHPWYGERKVCIREPASVS
                     HSTRNGGMCVISLAFVEAGELAFPEARDSHGAQSLIAADALQAAAIDDFAESFSVDQM
                     PAFVTSDALSVFGGGLGNIESALGSARGLLANPLGSLQSRLPGLIGDSAGLAGSVFGM
                     FRRGDSVLTAARGLFGGAGVYSMNRDAVRALLGLSQRFQAPARPASLSPSRQRSIENE
                     RAANALMSRAMLVQAAGMTATMPLPVYDDAVEIRKQFNGALDVESLTASDEVYTALQD
                     LRAKVHADITNRLASSARLQTIRPKGVTPALVLAYDHYGDVRREQELVEANRLSRPGF
                     VPAEPLKVLAV"
     gene            complement(1807..2358)
                     /locus_tag="VM406_01740"
     CDS             complement(1807..2358)
                     /locus_tag="VM406_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74711.1"
                     /translation="MSKLKKVGLWVLGIFVGLTILGGIIGESPEAQQAATPAEYPPVA
                     AQPVAAVADPEAERKAAKEQAHERARAISAKALEGIKLAPKWESVEARKVEADYYHVV
                     IWYKRDPSSYGEVERDTKEVARALLREIQSAGALDEKNYFMVVAVSGRMKEKGETGRD
                     MVRLFGETTYDISKDSLKFKPSA"
     gene            complement(2418..>3300)
                     /locus_tag="VM406_01745"
     CDS             complement(2418..>3300)
                     /locus_tag="VM406_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74712.1"
                     /translation="LSPHVMPLVEGIVEWVAANKELIRAEVGAWIRDVAASIKGWLAS
                     GGMDRLKAGIESLVSGIGSFVNAMGGMRNVMIAFGVLIALGPAASIAQLAAVFVQIGT
                     YVAPLVVKSLLVIGAAFKSVALALLTNPIGLVVTGIALAAGLLIYNWDKIGPWFASLW
                     TNVQGYFRGAWEGIKTLLSWSPLGLVMKTWEPLVSWFSGLWDRVKGFIEPIVSAGKAV
                     GGWFGGGKQAQQSGGLAGGRSPLVAAGGLASQANVSGEMRVRFENAPPGMRVDQGKTN
                     QSALAFNPDVGYRMAAY"
BASE COUNT          566 a         1172 c         1002 g          560 t
ORIGIN      
        1 cgtcgatcgc atcgccgccg atctggcccg gccctacggg atcgaggtgc aggcgcaggt
       61 gcccgtcggc gcgccgatga cgcacgcgat cgactcgaac accgactcgc cctggtcgat
      121 cgcgtgcgtc atcggcgcgc cgacgggcac ctgcgcctgc acctcgatcc cgtagggccg
      181 ggccagatcg gcggcgatgc gatcgacgcg cgcgccgctc cagcgccccg gcgaatgcac
      241 cgccgagcag tcgaccaggt cggccgtctt gctcctgccc ttcacgccaa ccgagacgga
      301 cttgccgtcg tagcggatcg gcgtggcgtc cacgtagccg gtgagcagct tgtccttgcc
      361 gatgaacagc tcgcaaacat cgcccgggcg aatccgccgc ggcagatcct gctggccggg
      421 ccacctgtcg gtcacggcaa gcgtgaagtc gcgcacctgg cgctcgatgc cggcggtgat
      481 ctcaatgtct ttccagccgc catactcctt gccgttcacc agcagtcgga cgtcgttcat
      541 accgcgagca ctttcaaggg ttcagcaggc acgaagccgg gccgggacag gcggttggct
      601 tccaccagct cctgctcccg gcgcacgtcg ccgtagtggt cgtacgccag caccagcgcc
      661 ggcgtcacgc ccttgggccg gatcgtctgc agccgggccg acgaagcgag gcggttcgtg
      721 atgtcggcgt gcaccttcgc gcgcaggtcc tgcagcgcgg tgtagacctc gtcgctggcc
      781 gtcagcgact cgacatcgag tgcgccgttg aactgcttgc ggatctcgac cgcgtcgtca
      841 tagaccggca gcggcatcgt ggccgtcatg ccggcggcct gaaccagcat cgcccggctc
      901 atcagtgcat tcgccgcgcg ctcgttctcg atcgagcgct gccggctggg cgagagcgag
      961 gccgggcgcg ccggcgcctg gaatcgctgg ctgaggccga gcagtgcgcg cactgcgtcg
     1021 cggttcatcg aatagacgcc cgcaccgccg aacagaccgc gcgcggcggt cagcaccgaa
     1081 tccccgcgtc gaaacatccc gaagaccgag ccggcaaggc cggcagagtc gccgatcagc
     1141 ccgggcaggc gggattgcag cgagccgagc ggattggcca gcaggccgcg tgcggatccc
     1201 agcgccgact cgatattgcc gaggccaccg ccgaacaccg acagcgcgtc gctggtgaca
     1261 aacgccggca tctggtcgac cgagaagctc tcggcgaagt catcgatcgc cgccgcctgc
     1321 agcgcgtccg cagcaatcag ggactgcgcg ccgtgcgagt cgcgcgcttc cgggaacgct
     1381 agctcgcccg cttcgacgaa cgccagcgag atcacgcaca tgccgccatt tctggtgctg
     1441 tgcgagacgg acgccggctc gcggatgcag accttgcgct cgccgtacca cggatggatc
     1501 agcgtgccgg gcccggcctc ctcgatggcg gcaagcaggc gatcccgctt gtccatgtag
     1561 tcgggaccga ccaggaacgc ggtgatgttc atctcccgcg ccgcgcgccc caggtcctcg
     1621 acatagggct ggtcccggtg cggatactcg tgcacctgga cgcgccggcc gaaggtggcg
     1681 tcgtcggact cgacttcgaa gggcacgccg cggaaggatg ccggctgcag tttgtctttc
     1741 caagccatgt gtccgcccaa ataaaaaagg cacccgaagg tgcctttctc tcgctgccca
     1801 aacccgctat gcgctgggct tgaacttcag tgaatctttc gagatgtcgt atgtcgtctc
     1861 gccgaacagc cgaaccatgt cacggccggt ttcacccttt tccttcatcc ggccgctgac
     1921 cgccaccacc atgaagtagt ttttctcgtc aagggcacca gcggactgaa tctcacgcaa
     1981 gagcgcgcgt gccacttcct tcgtgtcacg ctcaacctcg ccatagctgc tgggatcgcg
     2041 cttgtaccag atgaccacgt gatagtagtc agcttcgacc ttgcgcgcct cgaccgactc
     2101 ccacttcggc gccagcttga tcccctccag tgccttggcc gaaatcgccc ttgcgcgttc
     2161 gtgtgcctgc tctttggctg ctttccgttc tgcctccggg tccgcaaccg ccgccaccgg
     2221 ctgagccgcc acaggcggat actcggccgg cgtggccgcc tgctgcgctt ccggcgactc
     2281 gccgatgatg ccacccaaga tggtgaggcc gacgaaaata cccagcaccc acagaccgac
     2341 tttcttcagc tttgacatag cactcccctt attgccgtga aaagttttgt ttccggcaat
     2401 atagcagagc cgtcgcttta gtaagcggcc atccggtacc cgacgtcggg gttgaaagcc
     2461 agcgcacttt gattcgtttt gccctggtca acgcgcatcc ccggcggcgc gttctcgaag
     2521 cgcacccgca tctcgccgct cacgttggcc tgactggcga gtccgccggc agctacaagc
     2581 ggcgagcgcc ctcctgccag cccgccagac tgctgcgcct gcttcccgcc gccaaaccag
     2641 ccgccaaccg ccttgcctgc actgacgatc ggctcgatga agccctttac acggtcccac
     2701 aaaccagaga accagctgac aagcggctcc caggtcttca tcaccagacc aagcggagac
     2761 caggacaaca gtgtcttgat gccttcccat gcgcctctga agtagccctg cacgttggtc
     2821 cagagggatg caaaccacgg gccgatcttg tcccagttgt agatcagcag gccggccgca
     2881 agcgcgatcc ccgtgaccac cagtccgatc gggtttgtca gcagcgcgag ggccacgctc
     2941 ttgaaggcgg cgccaatcac gagcagggac ttcaccacca gaggcgccac gtaggttccg
     3001 atctggacga acacggctgc cagctgtgcg atcgaggcgg cgggcccgag ggcaatcagg
     3061 acgccgaagg cgatcattac gttccgcatg ccgcccatcg cattgacgaa cgatccaatg
     3121 cccgagacca gcgactcgat gcccgccttc aggcggtcca tcccgccgct ggcgagccag
     3181 cccttgatcg acgcggccac atcgcggatc caggcaccga cctccgcgcg gatcagttcc
     3241 ttgtttgcgg ccacccactc gacgatgccc tcgaccagcg gcatcacgtg cggtgacaac
//
LOCUS       DATLGF010000053         2130 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_223004, whole genome shotgun sequence.
ACCESSION   DATLGF010000053 DATLGF010000000
VERSION     DATLGF010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223004"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /gene="accD"
                     /locus_tag="VM406_01750"
     CDS             <1..661
                     /gene="accD"
                     /locus_tag="VM406_01750"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HVL74713.1"
                     /translation="GGRYEIGQEALPVDTLKFKDSKKYPDRLKEAMEATGETDALVVL
                     GGSIMSLPVVVACFEFEFMGGSMGSVVGERFVRGAQTALEQKVPFICITATGGARMQE
                     GLLSLMQMAKTTAMLTKLAEKKLPFISVLTDPTMGGVSASFAFMGDVVIAEPKALIGF
                     AGPRVIENTVREKLPEGFQRSEFLLQKGAIDMIVDRRKMREEIARLLALLQNQPAEVL
                     A"
     gene            737..2041
                     /gene="folC"
                     /locus_tag="VM406_01755"
     CDS             737..2041
                     /gene="folC"
                     /locus_tag="VM406_01755"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HVL74714.1"
                     /translation="MQETPHTLAAWLTRLEAMHPTAIDMGLERVGQVKERLGIGFECP
                     VITVGGTNGKGSTCAMLESILLQAGYRVGLYTSPHLIDFNERARINGEPVSDGALVEQ
                     FAAVDAQRGDISLTYFEFTTLAVLRLFSQAGLDAVILEVGLGGRLDAVNVIDADVAIV
                     TSVDIDHAEYLGDTREKIGFEKAGIYRSGRTAICGDPVPPRSLVEHAEAIGADLWLFG
                     RDFNYAGDKQQWNYGGRGMRRSSLAYPSLRGANQLLNASAALAALEALRHRLPVGAQE
                     VRTGLVLVDLPGRFQVLPGRPSVILDVAHNPHAAATLAQNLDNMGFHRYTFAVFGAMQ
                     DKDIEGVGAQLKDRVDHWCLTDLPLPRAARAADLAQRLEKLGVTPSREAGAERSVETF
                     ATPAEAFASAQKRAGESDRIAVFGSFLTVAGVMQAIRQEKAS"
BASE COUNT          384 a          654 c          713 g          379 t
ORIGIN      
        1 aggcggtcgt tatgagatcg gccaggaagc gctgccggtc gacacgctca agttcaagga
       61 cagcaagaag tacccggacc gcctgaagga agcgatggaa gccaccggtg aaaccgatgc
      121 gctggtcgtg ttgggcggat caatcatgag ccttccggtc gtcgtcgcct gcttcgaatt
      181 cgagttcatg ggcggctcga tgggttcggt ggtcggcgag cgcttcgtgc gcggcgcgca
      241 gaccgctctg gaacagaagg tgccgttcat ctgcattact gcgaccggcg gcgcgcgcat
      301 gcaggaaggc ctgctgtcgc tgatgcagat ggccaagacc actgcaatgc tgaccaagct
      361 cgccgaaaag aagctgccct ttatttcggt gctgaccgac cccaccatgg gtggcgtgtc
      421 cgcttcgttc gccttcatgg gcgatgtcgt gatcgccgag ccgaaggcgc tgatcggctt
      481 cgccgggccg cgcgtgatcg agaacacggt ccgcgagaag ttgccggaag gcttccagcg
      541 ttcggagttc ctgctgcaaa agggcgcgat cgacatgatc gtcgaccgcc gcaagatgcg
      601 tgaagagatc gcgcgcctgc tggcgttgct gcagaaccag ccggcggaag tgctggccta
      661 ggaagatacg ccattgcgca cggcgggcac ggcgtcatgg ccgtcctcgc cgtgtttcat
      721 ttcaggatcg tcattcatgc aagagactcc ccataccctc gccgcctggc tcacgcggct
      781 cgaagccatg catccgacag cgatcgacat gggcctggaa cgcgtcgggc aggtcaagga
      841 gcgcctcggg attggattcg agtgccccgt aatcaccgtg ggcggcacca acggcaaggg
      901 gtcgacctgc gcgatgctcg aatccatcct gctgcaggcc ggctatcggg tcggcctgta
      961 tacctctccc cacctgatcg acttcaacga gcgcgcgcgg atcaacggcg agccggtgtc
     1021 ggacggggcc ctggtcgagc agttcgcggc ggtcgatgcc cagcgcggcg acatttccct
     1081 cacgtacttc gagttcacga cactggcggt cctgcgcctg ttctcgcagg ccggcctcga
     1141 tgcggtgatc ctcgaagtgg ggctgggcgg ccgccttgat gcggtgaacg tgatcgacgc
     1201 cgatgtggcg atcgtcacca gcgtcgacat cgaccacgcc gagtatctgg gcgacacccg
     1261 cgagaagatc ggcttcgaga aggccggcat ctaccgctcc ggacgcaccg cgatctgcgg
     1321 cgatccggtg ccgccgcgtt cgctggtcga gcacgccgag gcgatcggtg ccgacctctg
     1381 gttgttcggg cgcgacttca actatgccgg cgacaagcag cagtggaatt acggcgggcg
     1441 cgggatgcgg cgcagttcgc tcgcgtatcc gagcctgcgt ggagccaatc agctgcttaa
     1501 tgcgtcggcc gcgctggccg cgctcgaggc gctgcgccac cgcctgccgg taggggcgca
     1561 ggaggtgcgc acaggcctgg tcctggtcga tctgcccggg cgattccagg tgttgccggg
     1621 gcgccccagc gtgatcctgg atgtcgcgca taatccacac gccgccgcga cgctcgccca
     1681 gaatctcgat aacatgggtt tccacagata cacattcgcc gtgttcggtg cgatgcagga
     1741 caaggatatc gagggagtgg gggcccaact gaaggaccgc gtcgaccact ggtgcctgac
     1801 cgatctgccg ctgccgcgtg ccgcccgggc cgcggatctg gcgcagcgcc tggaaaaact
     1861 gggggtgacg ccaagccggg aagccggggc agaacgcagc gtagagacat ttgccacacc
     1921 cgccgaagcg tttgccagcg cgcaaaagcg ggcaggcgag agtgatagaa ttgcggtctt
     1981 cggatcattc ctgacggtgg ccggcgtgat gcaggcaatc aggcaggaaa aagcgtcctg
     2041 aatcttgcca aggcggcagg ctttccagca tcttcagatg ggcctatttc tcgcatgagc
     2101 ttgttctcgt tcttccgcaa aaacaagcag
//
LOCUS       DATLGF010000054         7464 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_159802, whole genome shotgun sequence.
ACCESSION   DATLGF010000054 DATLGF010000000
VERSION     DATLGF010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7464
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159802"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..895)
                     /gene="ubiE"
                     /locus_tag="VM406_01760"
     CDS             complement(161..895)
                     /gene="ubiE"
                     /locus_tag="VM406_01760"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HVL74715.1"
                     /translation="MTNTTHFGYQTVPEEEKVRKVAEVFHSVANKYDVMNDLMSGGLH
                     RIWKAFTIAQAGVRPGFKVLDIAGGTGDLAKAFAKQAGPSGEVWLTDINESMLRVGRD
                     RLLNKGITSPALLCDAEKLPFPSNYFDRVSVAFGLRNMTHKDAALAEMQRVLKPGGKL
                     LVLEFSKVRDALQKPYDVYSFSVLPWLGKQIANDAESYRYLAESIRMHPDQETLKQML
                     QQAGLERVEYFNLTAGVAALHTGIKL"
     gene            complement(996..1376)
                     /locus_tag="VM406_01765"
     CDS             complement(996..1376)
                     /locus_tag="VM406_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HVL74716.1"
                     /translation="MNRVPTSLTVHKQSRVLEVAFDSGETFSLPFEYLRVYSPSAEVK
                     GHGPGQEVLQTGKRDVLLTALEPVGHYAVQPHFSDGHNSGIYTWDYLYALGADYERNW
                     SDYLRRLQAAGFPGESGRDAPAVT"
     gene            complement(1376..1816)
                     /locus_tag="VM406_01770"
     CDS             complement(1376..1816)
                     /locus_tag="VM406_01770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HVL74717.1"
                     /translation="MTAMPECELCREPGGEELYRDDNYRIVLVDEARYPGFCRVIWNA
                     HVREMTDLSTADRAILIAAVWQVEEAVREAMAPHKVNVASLGNVVPHLHWHVIPRYED
                     DAHFPNPIWGGVRRETPAALLAERRGRVERLREILRERFEQSLL"
     gene            complement(1819..5829)
                     /locus_tag="VM406_01775"
     CDS             complement(1819..5829)
                     /locus_tag="VM406_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HVL74718.1"
                     /translation="MNAPAQIQALMSDDQGAEHESNAPVRLREIPYNYTSFSDREIVM
                     RLLGEDAWRVLDELRSERQTGRSARMLYEVLGDIWVVRRNPYLQDDLLENPKRRAALI
                     DALNHRLGEVEKRRLASGLENAGDPAAIRRSANVDLLVKAARKAVAEFADDFRTTLEL
                     RKRTVKALGRHTAKDNIKFDGLSRVSHVTDATDWRVEYPFVVLTPDSEDEMAGLVKAC
                     IELGLTIIPRGGGTGYTGGAVPLTPLSAVINTEKLEQLGAVEMTMLPGVHRPYATIYT
                     GAGVVTKRVSDAAEKAGFVFAVDPTSAEASCIGGNIAMNAGGKKAVLWGTALDNLASW
                     RMVDPNGDWLEVTRIDHNLGKIHDAPMARFKLEWTHPSEKGAKLGAPFKSEILEIEGR
                     RFRKEGLGKDVTDKFLAGLPGVQKEGTDGLITSARWILHRMPKFARTVCLEFFGQARD
                     AIPSIVEIKDYLDAEAKKGGAILAGLEHLDERYLRAVGYATKSKRGVLPKMALIGDIV
                     GDDEDAVARAASEVIRMANTRVGEGFVAVSPETRKKFWLDRARTAAIAKHTNAFKINE
                     DVVIPLDRMGEYTDGIERINIELSIKNKLQFLAELRGFFAGGHLPVGKSDDADAHDIP
                     PEELLGDRVNQAARLIEDTHARWSYLLSNLDQPLTAARASLEALGVSLTWIDERIGRQ
                     PDATVFHVLQDRTIRISWKSEIRAPLRQIFNGGAFKQILDECTAIHKRVLRGRVFVAL
                     HMHAGDGNVHTNIPVNSDHYQMLQDAHAAVARIMALARSLNGVISGEHGIGITKLEFL
                     TEDEIKDFRDYKLRVDPEGRFNKGKLLNDPSMPSDLRNAYTPSFGLMGHESLIMQQSD
                     IGAIANSVKDCLRCGKCKPVCATHVPRANLLYSPRNKILATSLLVEAFLYEEQTRRGV
                     SIKHWEEFEDVADHCTVCHKCVNPCPVDIDFGDVSMNMRSLLRKMDKKSFNPGTAAAM
                     FFLNATDPATINATRAAMTGIGFKAQRLANDVLKKFAKKQTKAPPATHGKPPVKEQVI
                     HFINKKMPGNLPKKAARALLDIEDDKIVPIIRDPKKTTADTEAVFYFPGCGSERLFSQ
                     VGLATQAMLWHVGVQTVLPPGYLCCGYPQRGAGQFDKAEKMMTDNRVLFHRVANTLNY
                     LDIKTVVVSCGTCYDQLQSYEFEKIFPGCRIIDIHEYLLEKGVKLEGVGGVRYMYHDP
                     CHTPMKQQDPLKTVNALITTTDGQKVEKNERCCGESGTFGVSRPDISTQVRFRKEEEM
                     RKGADKLRADGFDGEVKILTSCPSCLQGLSRYNEDAGSTADYIVVEMAKHLLGENWLP
                     EYVARANDGGIERVLV"
     gene            complement(5977..6468)
                     /locus_tag="VM406_01780"
     CDS             complement(5977..6468)
                     /locus_tag="VM406_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HVL74719.1"
                     /translation="MIESAILWLLNLLAVPEVGLTSVFIVSLLSATLLPMGSEPAVFA
                     VIKVNGAMFWPVIAVATVGNTIGGAIDYWMGYGAKQAFASGANPRWLGWFERFGPKTM
                     LLAWLPGIGDPLCTLGGWLKMPFWPCVTYMAIGKFLRYVTMTTVLLYIPDGVWQFIGS
                     LLI"
     gene            6596..>7464
                     /gene="ilvA"
                     /locus_tag="VM406_01785"
     CDS             6596..>7464
                     /gene="ilvA"
                     /locus_tag="VM406_01785"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HVL74720.1"
                     /translation="MTIDYLQKILTARVYDVAIESPLEFAPLLSQRTENHIYLKREDV
                     QSVFSFKLRGAYNKMAHLKPAQLKRGVICASAGNHAQGVALSASRLSCRAVIVMPTTT
                     PPVKINAVKALGGEVVLFGDSYSDAYNHALTLEKKEKLTFVHPFDDPDVIAGQGTIGM
                     EILRQHQAPIHAIFAAIGGGGLISGVAAYVKAVRPDIKIIGVQSTDSDAMARSLKAGR
                     RVALPDVGLFCDGTAVKLVGEETFRLAKKYVDEIILVDTDEVCAALKDVFQDTRSILE
                     PAGALAVAGAKAYV"
BASE COUNT         1279 a         2387 c         2385 g         1413 t
ORIGIN      
        1 gcttgctgtt gctgcggcgg tgcctgacgc tgcagaccct gcgactgctt gccgaaggac
       61 ccgccgccgc ccaggcggcg cgcttcggcg tcggcggctg ccagcgacag gccgcttgtt
      121 gagatgacca gtgcgatcag aacctttttc atcccagctc ctagagtttg atgccagtgt
      181 gcaaggcggc caccccggcc gtcaggttga agtattcgac acgttccagt ccagcttgtt
      241 gcaacatctg cttcagcgtc tcctgatcag gatgcatgcg gatcgattcg gcgaggtaac
      301 ggtagctttc ggcgtcattg gcgatctgct tgcccagcca cggcagcacc gagaacgaat
      361 agacgtcata cggtttctgc agtgcatccc ggaccttgga gaactcgagc acgagcagct
      421 tgccgccagg tttgagcacc cgctgcatct ccgccagcgc ggcgtccttg tgcgtcatgt
      481 tgcgcaggcc aaaagccacg ctcacgcggt cgaagtaatt cgacgggaac ggaagtttct
      541 cggcgtcgca gagaagagcg gggctggtga tccccttgtt caacagtcgg tcgcgaccga
      601 cgcgcagcat cgattcgttg atgtcggtca gccacacctc gccgctcggg cccgcctgct
      661 tcgcgaacgc cttggcaaga tcgccggtcc cgccggcgat gtcgagcacc ttgaagccgg
      721 ggcgcacgcc cgcctgcgca atcgtgaacg ccttccagat ccggtgcagt ccgccggaca
      781 tcaggtcgtt catcacatcg tacttgttcg caacggaatg gaagacttcc gcaaccttgc
      841 ggactttctc ttcctcgggg acggtctggt agccgaagtg ggtggtgttg gtcatcgccg
      901 ctgtctctgt tggcaggcgg tcattataca agtgcgatgc agccctcacg tcgcgccagt
      961 ccaacggccg cgcaatcgcg gccgcaccca ttgcttcagg tcacagccgg tgcgtcgcgg
     1021 ccgctttctc cgggaaaacc tgccgcctgc agacgccgca gatagtcgct ccagttgcgc
     1081 tcgtaatcgg cacccagcgc gtacagataa tcccaggtat agatgccgga gttgtggccg
     1141 tcggaaaaat gcggctggac cgcatagtgg cccaccggtt cgagcgcggt cagcagcacg
     1201 tcgcgcttgc cggtctgcag cacttcctgc ccggggccgt gccccttgac ctcggcggaa
     1261 ggggagtaga cgcgcaggta ctcgaacggc agggagaagg tttcgccgga atcgaacgcc
     1321 acctccagca cgcgcgattg cttgtgcacg gtgagtgagg tcgggacgcg gttcatcaga
     1381 gcagcgactg ttcgaaacgc tcgcgcagga tctcgcgcag gcgttccacc cgcccgcggc
     1441 gttcggccag cagcgccgct ggcgtttcgc gcctgacacc gccccagatc gggttcggga
     1501 aatgcgcgtc gtcctcgtag cgcggaatca catgccagtg cagatgcggc acgacattgc
     1561 cgaggctggc aacattgacc ttgtgcggcg ccatcgcctc gcgcaccgct tcctcgacct
     1621 gccagacggc tgcgatcagg atcgcgcggt cggcggtgga caggtccgtc atctcgcgca
     1681 cgtgggcatt ccagatcacc cggcagaagc ccgggtagcg ggcttcatcg accagcacga
     1741 tgcggtagtt gtcgtcccgg tagagctctt ctccaccggg ctccctgcac agttcgcatt
     1801 cgggcatggc cgtcatcgtc acaccagtac gcgctcgatg ccgccgtcgt tggcgcgcgc
     1861 aacatactcg ggcagccagt tctcgcccag caggtgcttg gccatctcga cgacgatgta
     1921 gtccgcggtg ctgcctgcat cctcgttgta gcgcgacagg ccctgcaggc acgacgggca
     1981 ggaggtcagg atcttcactt caccgtcgaa gccgtcggcg cgcagtttgt cagcgccctt
     2041 cctcatctcc tcttccttgc gaaagcgcac ctgggtggag atgtcgggac gcgagacgcc
     2101 gaacgtgcca gattcgccgc agcagcgttc gttcttctcg accttctgcc cgtctgtcgt
     2161 cgtgatcagc gcgttgaccg tcttcagcgg atcctgctgc ttcatcggag tgtggcaggg
     2221 gtcgtggtac atgtagcgca cgccgcccac gccttcgagc ttgacgccct tctccagcag
     2281 atactcgtgg atgtcgatga tccggcagcc ggggaagatc ttttcaaact cgtagctctg
     2341 caactggtcg tagcaggtgc cgcaggagac cacgacggtc ttgatgtcca ggtagttcag
     2401 cgtgttcgca acacggtgga acagcacccg gttgtccgtc atcatctttt cggccttgtc
     2461 gaactggccg gcgccgcgtt gcggatagcc gcagcacaga tagcccggcg gcagcacggt
     2521 ctgcacgccg acatgccaga gcatcgcctg cgtggccagg ccgacctgcg agaacaggcg
     2581 ctcggagccg cagcccggga agtagaacac ggcttcggtg tcggcggtgg tcttcttcgg
     2641 gtcgcggatg ataggaacga tcttgtcgtc ctcgatgtcg agcagcgcgc gcgcagcctt
     2701 cttcggcagg ttgcccggca tcttcttgtt gatgaagtgg atcacctgct ccttcaccgg
     2761 cggcttgccg tgggtcgcag gcggcgcctt ggtctgcttc ttcgcgaact tcttcagcac
     2821 gtcattggcc aggcgctgcg ccttgaagcc gatgccggtc atcgccgcgc gggtggcgtt
     2881 gatcgtcgcc gggtcggtcg cattcaggaa gaacatcgcg gcggccgtac cgggattgaa
     2941 ggacttcttg tccatcttgc gcagcaggct gcgcatgttc atcgacacgt cgccgaaatc
     3001 gatgtcgacc gggcagggat tcacgcactt gtggcagacc gtgcagtggt cggccacgtc
     3061 ctcgaattct tcccagtgct tgatcgatac gccgcgccgg gtctgctctt cgtacaggaa
     3121 ggcttcgacc agcagcgagg tcgcgaggat cttgttgcgc ggcgagtaca gcaggttcgc
     3181 gcgcggcaca tgggtggcgc acaccggctt gcacttgccg cagcgcaggc agtccttcac
     3241 gctgttggca atcgcgccga tatcgctctg ctgcatgatc agagattcat gccccatcaa
     3301 accgaaggaa ggcgtgtagg cattgcgcag gtccgacggc atggacggat cgttcagcag
     3361 cttgcccttg ttgaagcgcc cttccggatc gacgcgcagc ttgtagtcgc ggaaatcctt
     3421 gatttcgtct tcggtcagga actccagctt cgtgatgccg atgccgtgct cgcccgagat
     3481 cacgccgttg agcgagcgcg ccagcgccat gatgcgcgcg accgcggcat gcgcgtcctg
     3541 cagcatctgg tagtggtcgg aattgacggg gatgttggtg tggacgtttc cgtcgcccgc
     3601 atgcatgtgc agcgcgacga acacgcggcc gcgcaacacg cgcttgtgga tcgcagtgca
     3661 ctcgtcgagg atctgcttga acgcgccgcc gttgaagatc tggcgcagcg gcgcgcggat
     3721 ttccgacttc caggagatgc gaatcgtgcg gtcctgcagc acgtggaaca ccgtggcatc
     3781 cggctggcgg ccgatgcgct cgtcgatcca ggtcagcgac acgcccagcg cttcgaggct
     3841 cgcgcgtgca gccgtcagcg gctggtcgag gttggacagc aggtaggacc agcgcgcatg
     3901 cgtgtcttcg atcaggcgcg cggcctggtt gacgcggtcg ccgagcagct cttccggcgg
     3961 gatgtcgtgt gcatcggcat cgtcgctctt gccaaccggc agatggccgc cggcgaagaa
     4021 accgcgcaat tcggccagga actgcagctt gttcttgatc gacagttcga tgttgatgcg
     4081 ctcgatgccg tcggtgtact cgcccatgcg gtcgagcgga atcacgacgt cctcgttgat
     4141 cttgaacgcg ttggtgtgct tggcgatcgc ggcggtgcgt gcgcggtcca gccagaattt
     4201 cttgcgcgtc tccgggctca cggcgacgaa accttcgccg acgcgggtat tggccatgcg
     4261 gatgacttcg gaagccgcgc gtgcgaccgc gtcctcgtcg tcgccgacga tgtcgccgat
     4321 cagcgccatc ttgggcagca cgccgcgctt ggacttggtc gcatagccga ccgcgcgcag
     4381 gtagcgctcg tccaggtgct cgagaccggc gaggatggcg ccacccttct tcgcctcggc
     4441 atcgaggtaa tccttgattt cgacgatgga cggaatcgcg tcgcgcgcct ggccaaaaaa
     4501 ctccaggcag acggtgcgcg cgaacttcgg catccggtgc aggatccagc gcgccgaggt
     4561 gatcagcccg tcggtgcctt ccttctgcac gccgggcaag ccggcaagga acttgtcggt
     4621 gacgtccttg cccagccctt ccttgcgaaa gcgccggcct tcgatttcca ggatttcgga
     4681 cttgaacggc gcgccgagtt tggcgccctt ttcgctcgga tgggtccatt caagcttgaa
     4741 ccgcgccatc ggcgcgtcat ggatcttgcc gaggttgtgg tcgatgcgcg tgacttccag
     4801 ccagtcgccg ttcgggtcga ccatgcgcca ggacgcgagg ttgtccagag ccgtgcccca
     4861 cagcacggcc ttcttgccac ccgcgttcat cgcgatgttg ccgccgatgc acgaggcttc
     4921 cgccgaggtc gggtcgaccg caaacacgaa gccggccttc tcggccgcgt ccgagacgcg
     4981 cttggtcacc acgccggcgc cagtgtagat cgtcgcgtac ggacgatgca cgcccggcag
     5041 catggtcatt tccaccgcgc ccaactgctc cagcttctcg gtgttgatca cggccgacag
     5101 cggcgtcagc ggtaccgcgc cgccggtata gccggtgccg cctccgcgcg gaatgatggt
     5161 gagcccgagt tcgatgcagg ccttcaccag gccggccatc tcgtcctcgg aatccggggt
     5221 cagcacgacg aacggatatt cgacgcgcca gtcggtcgcg tcggtcacgt gcgagacgcg
     5281 cgacaggccg tcgaacttga tgttgtcctt ggctgtgtgg cggccgagcg ccttcaccgt
     5341 gcgcttgcgc agctccagcg tggtgcggaa atcgtcggcg aactcggcca cggccttgcg
     5401 cgcggccttg accagcaggt cgacgttggc gctgcggcgg atggcggccg ggtctccggc
     5461 attctccagt cccgatgcca ggcggcgctt ttcgacttcg ccaaggcgat gattgagcgc
     5521 gtcgatcagc gcggcgcggc gcttcggatt ctccagcagg tcatcctgca ggtagggatt
     5581 gcggcgcacc acccagatgt cgcccagcac ttcatacagc atgcgggccg accggccggt
     5641 ctggcgctcg ctgcgcagct cgtcgaggac gcgccaggcg tcttcgccga gcaggcgcat
     5701 gacgatctcg cggtccgaaa acgaggtgta gttgtacggg atttcacgca ggcggactgg
     5761 cgcgttggac tcgtgctcgg caccctggtc gtcggacatc aaggcttgaa tttgggcggg
     5821 ggcgttcatc agggtgacgg gacagacgct aaaggcgcat tctaacgtat tgcgccgcaa
     5881 ccaaacccct acgccctctg aggggatggg tcaatatcat attgccaatg cttgatcgcg
     5941 cccagtccgc gcacgaatgt tacgccatga tgatgttcaa atgagcaggc tgccgatgaa
     6001 ctgccagacg ccgtccggga tgtagagcag cactgtcgtc atcgtcacgt agcgcaggaa
     6061 tttgccgatt gccatatagg tcacgcacgg ccagaacggc atcttcagcc agccgcccaa
     6121 tgtgcacagc ggatcgccta tgcccggcag ccaggcgagc agcatggtct tcgggccgaa
     6181 acgctcaaac cagccgagcc agcgcgggtt ggcgccgctg gcgaaggcct gcttggcgcc
     6241 atagcccatc cagtagtcga tcgccccgcc gatggtattg cccaccgtcg ccaccgcaat
     6301 caccggccag aacattgcgc cattgacctt gatcaccgcg aacacggcgg gctccgatcc
     6361 catgggcagc aaggtcgccg aaagcaggct gacaatgaag accgaggtca gccccacctc
     6421 gggaacggcc agaaggttca gcagccagag gatggcggat tcgatcatgc gaagacttga
     6481 tgcgacatag gcaggcaggt atgcaaagtg gacagcctgg aggcccggcg aaggcggtcc
     6541 attataatga cgggttccgc gcgcgctgcg cggatgcgct cttcaaaacc cgcctatgac
     6601 tatcgactac ctgcaaaaga tcctcaccgc acgcgtgtac gacgtcgcca tcgagtctcc
     6661 gctcgagttc gcacccctcc tgtcgcagcg gacggaaaac cacatatacc tgaagcggga
     6721 ggatgtgcag agcgtgttca gcttcaagct gcgtggcgcc tataacaaga tggcgcacct
     6781 gaagccggcc cagttgaagc gcggcgtgat ctgcgcgtcg gccggcaatc atgcgcaggg
     6841 cgtggcgctg tcagcctcgc gcctgtcctg ccgcgccgtc atcgtgatgc cgaccaccac
     6901 gcctccggtc aagatcaatg ccgtcaaggc cctcggcggc gaagtggtcc tgttcggcga
     6961 ctcctacagc gacgcctaca accatgcgct gacgctggag aaaaaggaaa agctgacctt
     7021 cgtacacccg ttcgacgatc cggacgtgat cgccggccag ggcacgatcg gcatggagat
     7081 cctgcgccag caccaggcgc cgatccatgc gatcttcgcc gccatcggcg gcggcggcct
     7141 gatttcaggc gtagccgcct atgtgaaagc ggtacgcccg gacatcaaga tcatcggcgt
     7201 gcagtcgacc gattccgatg cgatggcgcg cagcctgaag gccggccgcc gcgtagcgct
     7261 gcccgacgtc ggcctgttct gcgacggcac cgcggtcaag ctggtcggcg aagagacctt
     7321 ccgtctcgcg aagaagtatg tggacgagat catcctggtc gacaccgacg aggtctgcgc
     7381 cgcgctgaag gacgtgttcc aggacacgcg cagcatcctc gaaccggccg gcgcgctggc
     7441 ggtggccggc gccaaggcgt acgt
//
LOCUS       DATLGF010000055         2447 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_175817, whole genome shotgun sequence.
ACCESSION   DATLGF010000055 DATLGF010000000
VERSION     DATLGF010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175817"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VM406_01790"
     CDS             complement(<1..678)
                     /locus_tag="VM406_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVL74721.1"
                     /translation="MTDAIERHTVAMHFVSAAVAHLQPAAKERALAVAGIPAHLLDQP
                     NARVPANAYSALWLAVARELDDEFFGLDRRGMKVGSFALICQAVLSCADLDRALKRML
                     RGFALFLDEVHGELHLEGGDAVVRLTNTISQEDDRRFADETLLILVHGLMCWLAGRRI
                     PLRHVSFRHARPPYAREYTLMYCDDLTFDAPQTAMHFEGRALSLPVSQDTSTLQHFLR
                     TAPQSVFL"
     gene            787..>2447
                     /locus_tag="VM406_01795"
     CDS             787..>2447
                     /locus_tag="VM406_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802892.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HVL74722.1"
                     /translation="MTDLSAEYRALANYRPTHKVRFVTAASLFDGHDAAINIMRRILQ
                     SMGTEVIHLGHNRSVDEVVTAALQEDVQGIAISSYQGGHVEYFKYMVDLLKQRGGSHI
                     QVFGGGGGVIVPAEIRELQDYGVARIYSPEDGQRMGLPGMIGEMVMRCDKDVAQYAPR
                     ALDAIQGHGEQNWRALAQLITAIENGSADADLMKAVRAQADTRSVPVLGITGTGGAGK
                     SSLTDELVRRLRLDQDDNLRVAIISIDPSRRKSGGALLGDRIRMNAISPWQQGPRVYM
                     RSLATRDFGSEISAALPDVIGACKAAGFDLVIVETSGIGQGDAAIVPHVDVPLYVMTP
                     EFGAASQLEKIDMLDFAQFVAINKFDRKGAADALRDVAKQVQRNKEAFGRRPEEMPVF
                     GTMAARFNDDGVTALFQALKLRLAEIGLRLHEGKLPLVNVRHSSNQTPVVPPARTRYL
                     SEISDTVRAYKQRARRQAVIARELQQLRSSQRMLTSEVEDAAGPAADLLETLAAAREG
                     RLDGDARKLLAQWPALQKAYAGDEYVVKIRDKEIRTRLTSKSLSGT"
BASE COUNT          464 a          768 c          820 g          395 t
ORIGIN      
        1 gaggaacact gactgcggcg cagtacgcag gaagtgttgc aacgtcgatg tgtcctgaga
       61 caccggcagc gacagcgccc gcccttcgaa gtgcatggcg gtctgcggcg catcaaaggt
      121 gaggtcgtcg caatacatca aggtgtattc gcgcgcgtac gggggacgcg cgtgtcgaaa
      181 agacacatgc ctcagcggaa tgcgccggcc tgcaagccag cacatcagac catgcacgag
      241 gatgaggagt gtttcatcgg cgaaccggcg atcgtcctct tgcgaaatcg tgttcgtgag
      301 ccgcacgacc gcgtcgccgc cctccaggtg cagctctccg tgcacctcgt ccaggaacaa
      361 ggcaaagccg cggagcatgc gtttgagagc ccgatccagg tcggcacagg agagcaccgc
      421 ctggcagatg agggcgaaac tgccgacctt catcccgcgc cggtcgagcc cgaagaattc
      481 gtcatcgagt tcacgggcta cggccagcca aagcgcggag tacgcgttag cgggcacgcg
      541 cgcgttcggt tggtccagca agtgcgccgg gatgcctgcg acggctagcg cgcgctcctt
      601 cgcggcgggc tgcagatgag ctaccgccgc actcacgaaa tgcatggcga cggtgtgtct
      661 ctcgatggcg tcggtcatgg gcagcccgat tatcggggtg gcaaaaaatg ccacctcgct
      721 gcacccgcag gacatggtgc cccgtgggca ccgctcctaa agtggtgacg ctcctggaga
      781 ccgaccatga ccgacctgtc cgccgaatac cgcgcccttg ccaactaccg cccgacccac
      841 aaggtccgat tcgtcacggc cgcctcgctc ttcgacgggc acgacgccgc catcaacatc
      901 atgcggcgca tcctgcagtc catgggcacg gaagtgatcc acctgggcca caaccgcagc
      961 gtggacgaag tggtgacggc ggccctgcag gaagacgtgc agggcatcgc catcagctcc
     1021 tatcagggcg gccacgtcga atacttcaag tacatggtgg acctgctgaa acagcgcggc
     1081 ggatcgcaca tccaggtttt cggcggtggt ggtggcgtaa tcgtgccggc ggaaatccgc
     1141 gagttgcagg actacggcgt cgctcgaatc tatagcccgg aagacggtca gcgcatgggc
     1201 ctgccgggca tgatcggcga gatggtgatg cgctgcgaca aggacgtggc ccagtacgcg
     1261 ccccgcgcgc tggacgcgat ccagggccat ggcgagcaga actggcgcgc cctcgcgcaa
     1321 ctgatcaccg cgatcgagaa cggttctgcg gacgccgatc tgatgaaggc cgtgcgcgcg
     1381 caggcggata cgcgttccgt gccggtcctc ggcatcaccg gtaccggggg ggctggcaag
     1441 tcgtcgctca ccgacgaact ggtacgccgc ctgcgcctcg accaggacga caacctgcgt
     1501 gtggcgatca tctcgatcga tccatcgcgc cgaaagagcg gtggcgcgct gctgggggat
     1561 cgcatccgca tgaacgcgat ctcgccgtgg cagcaaggcc cgcgtgtcta catgcggtcg
     1621 ctcgccaccc gcgacttcgg cagcgagatt tctgcggcgc tgccggatgt gatcggcgcc
     1681 tgcaaggcgg ccggcttcga cctggtgatc gtcgagacct ccggcatcgg ccagggcgat
     1741 gcggcgatcg tgccgcacgt ggatgttccg ctgtacgtga tgacgcccga attcggcgct
     1801 gcgagccagc tggaaaagat cgacatgctg gacttcgcac agttcgtcgc gatcaacaaa
     1861 ttcgaccgca aaggcgcagc ggatgcgctg cgcgatgttg ccaagcaggt gcagcgaaac
     1921 aaggaagcgt ttggcaggcg gccggaggag atgcccgtgt tcggcacgat ggctgcgcgt
     1981 ttcaacgacg atggggtcac ggcgctgttt caggctttga agctgcgact cgctgagatt
     2041 gggctgaggt tgcacgaagg caagctgccc ctcgtgaatg tgcgccacag cagcaatcag
     2101 actccggtgg tgccgccggc gcgcacgcgc tacctctccg aaatttcgga tactgtcagg
     2161 gcctacaagc agcgcgcacg caggcaggca gtgatcgctc gcgagctgca acagctccgt
     2221 tcgtcgcagc ggatgctgac ttcggaagtt gaggacgccg ccggtccggc tgctgacctg
     2281 ctcgaaacgc tggcggctgc gcgcgagggg cggctcgacg gcgatgcgcg caagctgctg
     2341 gcccagtggc cggccctgca gaaggcctat gccggcgacg aatacgtggt aaagatccgc
     2401 gacaaggaga tccggacgcg gctcacgtcg aagtcgctct ccggcac
//
LOCUS       DATLGF010000056        12157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_207174, whole genome shotgun sequence.
ACCESSION   DATLGF010000056 DATLGF010000000
VERSION     DATLGF010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 12157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12157
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207174"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VM406_01800"
     CDS             complement(<1..196)
                     /locus_tag="VM406_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74723.1"
                     /translation="MNATSRSAEQEREHVIATVVLAFVKDPFARWLYPQAHQYLAHVP
                     RMTAALVGKAFENETADHIAG"
     gene            354..815
                     /locus_tag="VM406_01805"
     CDS             354..815
                     /locus_tag="VM406_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HVL74724.1"
                     /translation="MQVGEPQAMSICAAGPADFERLTVLWEASVRATHHFLTDDYLAL
                     LRDKVRNEYLAAVELRTCRNDAGDILGFIGVSGRKVEMLFVDPQRRGEGIGRRLMEYA
                     VREMGATEVDVNEQNPAAAGFYRHLGFEPVGRSPLDGLGRPYPVLHLRLVH"
     gene            1189..1956
                     /locus_tag="VM406_01810"
     CDS             1189..1956
                     /locus_tag="VM406_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HVL74725.1"
                     /translation="MSALPLSSMLHAADSTPVAVAARLGLVLALHAGFFYVLHQGLEQ
                     PPASMQTAKTVFVSFVPVAPEPVPEPQPRIEPEPQHVQQPDPPKHKPAPKPVVKPQPK
                     PVEKTPAPVLSEPSERAIEVPPAPPPEPAPAPAAAAPAAQAVAPAPAAPPGPPAPVAP
                     PAPRTITSGIQYLQAPAPEYPPASRRMGEEGQAVVRVFVNERGRPERAEIQDSSGSQR
                     LDEAARRAVLRAVFKPHIENGRPVAVFAVIPIRFQLD"
     gene            1982..2710
                     /locus_tag="VM406_01815"
     CDS             1982..2710
                     /locus_tag="VM406_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HVL74726.1"
                     /translation="MEVVNPYGLQNLWAQGDLLIKGVALLLLAMSVASWYVILTKAMQ
                     LWRYNRAAHAAGHEFWDATNIEDGLATLGTNNPFADIAKAGVVSMRHHNAHKGHLHDQ
                     LSVSDWVTLSLRQAIDEASAKLQSGMAILASVGSSAPFVGLFGTVWGIYHALVAIGTS
                     GQANIDKVAGPVGEALIMTALGLAVAIPATFGYNALVRGNKTVIAKLNKFGFDLHALF
                     VTGARADGDSGAAAPKLVPIRGGA"
     gene            2710..3126
                     /locus_tag="VM406_01820"
     CDS             2710..3126
                     /locus_tag="VM406_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HVL74727.1"
                     /translation="MAMGTLSENEDDFNPEINTTPLVDVMLVLLIIFIITIPVLNHAV
                     SIDLPRATNQPLEMKPQSISLTINQAGTVFWNDQPVDKEELAARIAGAAAMQPQPELH
                     MKVERTAQFETVAQVMAAAQSGGLGKIGFVTEAREN"
     gene            3414..4253
                     /locus_tag="VM406_01825"
     CDS             3414..4253
                     /locus_tag="VM406_01825"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HVL74728.1"
                     /translation="MTTTIGLLEAAFLGLIQGLTEFLPISSSAHLRIIGPFLPSGLDP
                     GAAFTAITQIGTELAVLIYFRHDILRIARAWLRSLFESDARGHVDARLGWLVILGSLP
                     IAILGLLFQDAIETYLRNLYLTAAMLVLFGLILGAADRYGKRRRSLDQMTWRDGILFG
                     FAQAMALIPGVSRSGGTITAGLIMGYSREAAARYSFLLAIPAVMASGLYQLYKSWGVA
                     GPVDAASTALATAVSFVVGYVVIVVFLRIVSSRGYWPFVLYRIVLGFSVMTLLGLGHL
                     TPY"
     gene            4351..5406
                     /locus_tag="VM406_01830"
     CDS             4351..5406
                     /locus_tag="VM406_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HVL74729.1"
                     /translation="MFLLVAYVLLALLFSFLCSIAEASLLSITPSYVADLKDKQPGRA
                     ELLKKLREDNIDRSLAAILTVNTIAHTVGAIGAGAQATLVFGSAWFGVFSAVMTLLIL
                     FLSEIVPKTLGAVYWRQLALPTAIYVNVLVKTMYPLIWVSEKLTQWIAGGQKSQPFSR
                     DEFVAMAGLGQQEGHIDERESKIIRNLFLFRSVDTGAVMTPRVVVAAVQQDTPVEQAL
                     EATESTPFSRLPVYETDLDTVTGFVLREDLLVAQNEGRGHVPVREFRRDLIAVVDSTP
                     LSRLLETLLAYRQHIAAVVGEYGETKGVVTLEDVVETLLGIEIVDEGDKIADMQQLAR
                     QMWARRAGRLGIRPEQT"
     gene            complement(5572..6018)
                     /locus_tag="VM406_01835"
     CDS             complement(5572..6018)
                     /locus_tag="VM406_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74730.1"
                     /translation="MNRRLARLIAGCCAMAAAVLLSAACDSRQEPSPSVSVSPQEQKL
                     LAEMNALGRVEAEGNSYKYRLLATCRLEVEESLEGSLLERHVVSLQDASYVPFAYATG
                     LGYALRLASDPRKSDIYRAMSLEQIERMQSLFDALTKTCIESAPAS"
     gene            complement(6015..7349)
                     /locus_tag="VM406_01840"
     CDS             complement(6015..7349)
                     /locus_tag="VM406_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HVL74731.1"
                     /translation="MEVLILLGLILLNGVFAMSEIALVTARRARLARLAEDGSRPAAV
                     AVALHDEPTRFLSTIQIGITSIGVLSGIVGESALAGPLATWFQSLGIAQATSQIAATT
                     AVVVIITYFAIVFGELVPKRMGQFNPEGIALLVARPMNGLSRLTRPFVRLLSASTDTT
                     LRLLGKREQQDQGVTEEEIHALLTEGSEAGVIERHEHAMVRNVFRLDERLVGSLMIPR
                     FDIVYLDVNLPIEENLKRITASEHSRFPVCRGGLRDVIGILSAKQVLSQTLMGGMPDL
                     AASAQPAVFVPEAMTGMALLEIFRASQTQMVLVIDEYGEVQGLVTLQDVLEAVTGEFT
                     PSNLDDAWAVQREDGSWLLDGMIPIPELKDKLELKSVPEEERGRYHTLSGLLMCLLER
                     VPQTADIATWDGWRFEVVDMDGKRIDKVLASRLPSPPTEAGPAGAAAEKDTP"
     gene            7950..10046
                     /locus_tag="VM406_01845"
     CDS             7950..10046
                     /locus_tag="VM406_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVL74732.1"
                     /translation="MTLPKTAIAASAIALPCLALPLPALAQAETLAPIVVTATAPGAD
                     TRDLPLSIDRLDQDDIQVGNPMVNLSETLNRVPGIVVRNRQNYAQDLQISSRGFGARA
                     TFGVRGVRLYVDGIPATMPDGQGQTSNFDLASAAHIDVLRGPFSALYGNSSGGVVSIY
                     TEDGQPGLALSPFAQFGSWNTRRYGIKSAGEHGMLNHVLSINRFETDGYRAHSAARRD
                     IANAKLRLVPGGDTIWTLVANRVSMPEVLDPLGLPREQMLAAPRSVHPNALLFNTRKS
                     VRQQQLGMNAEHFLNAQDSLHLMAYGGQRDVLQFLAIPPAPQAAPTHSGGVVDLGRDF
                     GGIDLRWAREAAAGWLWTAGLSVETVEEDRRGYENFIGDTPGVRGALRRNERNTITSI
                     EQYAQAQWEPTQQWLLLAGVRHSMVRVRTRDRYVAPGNPDDSGAIRYRATNPVLGATW
                     RATADLNVYASYGRGFETPTLNELSYRTSGNGAAGFNFSLRPATSSNIEVGIKAAVAQ
                     HWRLNLAAFHIDTRNELTVLSSAGGRAVFQNAASTRRRGLELGVEGRWDNGLGILLSA
                     SLLDARYADRNGATTFAGNRLPGVPARTGFAELSWRAPQGGFSTALELQHVGRVYVND
                     ANSEAADAYTIANARIGFEQQIGGWTLREFLRVDNLANRRHVGSVIVNEGNGRFYEPG
                     PGRHVLVGVTASHAWK"
     gene            10513..11244
                     /locus_tag="VM406_01850"
     CDS             10513..11244
                     /locus_tag="VM406_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74733.1"
                     /translation="MRFTQAFKAIPPVALATLCMLAASAAQAGRPMITDDAAILEANA
                     CQLETWYQATRSAGNEWWALPACNVGGYAEVAIGGARLREPDGRTTVAVIQAKALLRA
                     YDETRPGFGMVLGTERDRGVHRGSAGNPFIAGIASVPLRGEAMLLHANLGWQRVRDDF
                     AGTRENSATWALALDSEVAARTRVSLESYGQSRERAMAQFGVAYDLAPGRVQIDGSVG
                     SRIARGAPDRYATIGLVFFTDPLFR"
     gene            11313..>12157
                     /locus_tag="VM406_01855"
     CDS             11313..>12157
                     /locus_tag="VM406_01855"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVL74734.1"
                     /translation="MEREELIVYIQSLLEAQDAQTIRALMNRVSMADLAEALSNVPVE
                     DAVRFWNMLDQEERLELFVYFPHGIQDAILAQLPRPALVALFDQLPSDDRADIYKRMD
                     DEMREKLMPALAKVERDDILKLAAYEEGTVGSITTSDYATVQEDMTVAEALRVIRAGA
                     PDKETIYVVYVLDELRRLVGTVSLRELVLEPESTRLGDLMHREPIYAEADAPREEAAR
                     LISRYDLLAVPVVDAGRRMIGIVTVDDAMAVAQEETTEDFHKSGGMAAPEGMSVRSAS
                     VFQLYR"
BASE COUNT         2214 a         4019 c         3770 g         2154 t
ORIGIN      
        1 agccggcaat gtgatccgca gtttcgtttt cgaaagcctt gccgaccagc gctgcggtca
       61 tgcgcggcac gtgcgccagg tattgatgcg cttgcggata cagccagcga gcgaacggat
      121 ctttcacaaa cgcgaggacg accgtggcga tgacgtgttc tcgctcctgc tccgcgctgc
      181 gcgatgttgc attcatggcc tgccccttgc ggtgatgttc ctacagccta gtacaccgcg
      241 gccgccggta aaggcgacag gcggctttaa ccggcgcacg ctgggatctg gcgagcagct
      301 gccgtagact tgcgttcgtc agcagccctg cgcaaccgga ccgggaggag atcatgcagg
      361 ttggcgaacc ccaggccatg tcgatctgtg cggccggccc ggccgacttc gagcgtctca
      421 ccgtgctgtg ggaagcatcg gtgcgcgcga cccatcactt cctcaccgac gactacctcg
      481 cgctgttgcg cgacaaggtg cgcaacgaat acctcgccgc cgtcgagctg cgcacctgcc
      541 gtaacgacgc cggcgacatc cttggtttca tcggcgtcag cggtcgcaag gtcgagatgc
      601 tgttcgtgga cccgcagcgc cgcggcgagg gcatcggccg ccggctgatg gaatacgcgg
      661 ttcgcgagat gggggcgacc gaggtcgatg tgaacgagca gaacccggcg gccgccggct
      721 tctaccgcca cctcggtttc gagccggtcg ggcgatcgcc gctcgacggg ctcgggaggc
      781 cgtacccggt cctgcacctg cggcttgtgc actgaaccag cgccgttcaa ccggcgccgg
      841 cagcaatgcc acgcggaggg tgacgaagga tacgaggtaa gccagcgcga acaacaatcc
      901 gacctcgccg acggaacatc gcgccggtac agcgtccacc tggttgcgca gccgtcgtcg
      961 gatgcgcgac cgcgataccg tgaatgagaa tgattcgcac ttacggtata attgcgctac
     1021 acgaggcccc gaccgccatg gaactgaaga aaaatcaatg gtttacttgc gacccctgtc
     1081 ccgacggagc gcgcccgcgc caaaagccgg tcttatacta gttcgtcccc gattcattgg
     1141 cgcaccgctc ttgccagcac cggccccgca ttctgcaaag gacttagcgt gagtgccctc
     1201 cccctttcct ccatgctgca cgcggccgac agcacgcccg tggccgtcgc tgcccgcctg
     1261 ggcctcgtcc tcgcgctcca tgccggcttc ttctacgtgc tgcatcaggg cctggaacag
     1321 ccgcctgcta gcatgcagac cgcaaagacg gtctttgtct cgttcgtgcc cgtcgcaccc
     1381 gagccggtac ccgagccgca gccgcgcatc gagcccgagc cccagcacgt gcagcagccc
     1441 gatccgccca agcacaagcc ggcacccaag ccggtggtca aaccccagcc caagccggtc
     1501 gagaagacgc cggcgccggt actgagcgag ccgtccgagc gcgcgatcga agtgccgccc
     1561 gcaccgccgc cggaacccgc gccggcccct gccgccgccg cgccggcagc acaggccgtc
     1621 gcccccgcac ctgccgcgcc gccgggccca ccagcaccgg tcgcgccgcc cgcgccacgc
     1681 acgattacct ccggcatcca gtacctgcag gcgcctgctc ccgagtatcc gcctgcgtcg
     1741 cgccgcatgg gcgaggaagg ccaggcggtg gtgcgcgtgt tcgtcaatga gcgcggccgc
     1801 ccggaacgcg ccgaaatcca ggattcctcg ggctcgcagc gcctggacga agcggcccgc
     1861 cgcgccgtgc tgcgcgcggt gttcaagccg catatcgaaa acggccgccc ggtcgccgtg
     1921 ttcgctgtca tcccgatccg ttttcaactc gattaaaccg aatcaactca aggagctttt
     1981 catggaagtc gtcaacccgt atggcctgca gaacctgtgg gcacagggcg atctgctgat
     2041 caagggcgtg gccctgctgc tcttggccat gtcggtcgcg tcctggtacg tgatcctgac
     2101 caaggccatg cagctgtggc gctacaaccg cgccgcgcac gccgccggtc atgagttctg
     2161 ggacgccacc aacatcgagg acggcctcgc caccctcggc accaacaacc cgttcgccga
     2221 catcgccaag gccggcgtcg tctcgatgcg ccaccacaat gcccacaaag gacatctgca
     2281 cgaccagctg tcggtgtccg actgggtcac gctgtcactg cgccaggcga ttgacgaagc
     2341 ctctgccaag ctgcagtccg gcatggcgat cctcgcctcg gtcggttctt ccgccccgtt
     2401 cgtcgggcta ttcggcacgg tatggggcat ctaccatgcg ctggtggcca tcggcacctc
     2461 ggggcaggcc aacatcgaca aggttgcggg tccggtcggc gaggcgctga tcatgacggc
     2521 gctgggcctg gcggtcgcaa tccccgccac ctttggctac aacgcgctgg tacgcggcaa
     2581 caagaccgtg atcgccaagc tcaacaagtt cggcttcgac ctgcatgcgc tgttcgtcac
     2641 cggtgcgcgc gccgacggcg attccggggc agccgcgccg aagctggtgc cgatccgcgg
     2701 aggtgcatga tggcgatggg tacgctgtcc gagaacgagg acgacttcaa tccggagatc
     2761 aacaccacgc cgctggtcga tgtgatgctg gtgctgctga tcatcttcat catcacgatt
     2821 cccgtcctca atcatgcggt gtcgatcgac ttgccgcgcg cgaccaacca gccgctggag
     2881 atgaaaccgc aaagcatcag cctgacgatc aaccaggcgg gaacggtgtt ctggaacgac
     2941 cagccggtcg acaaggaaga actggccgcg cgcatcgctg gtgcagcggc gatgcagccg
     3001 cagcccgagc tgcatatgaa ggtggagcgt accgcccagt tcgaaacggt ggcgcaggtg
     3061 atggcagcgg cgcaatcggg cggcctgggc aagatcgggt tcgtgacgga ggcgcgggaa
     3121 aactgacgcg caagacctgc cgcaaatccg acaagtcgga ccagacgtct tgccgtatcg
     3181 gcatccctcg acggccagac ctcccccgtc aggcagcgga atgaagatct cccgccagcg
     3241 cgcctcgtag cgatcccctt cctgaacaaa gcggacatgc ggtgcggatc cggaccggcg
     3301 agacgattcc ggtccaattg ctccgacgca aaccggcgtt gactgtaagt ccggatcatg
     3361 gaagcatctt gcttcacgtc cacggcagtt gccacaacat ttcccaccga atcttgacca
     3421 ccaccatagg gcttctagaa gccgctttcc ttggcctgat acaggggctc accgaatttc
     3481 ttccaatttc gtccagcgcg catctgcgca tcattggccc cttcctgcct tcgggtctgg
     3541 accccggagc cgcattcacg gcgatcacac agatcgggac ggagcttgcg gtcctgatct
     3601 atttccgcca tgacattctt cgcattgcgc gcgcctggtt gcgctctctg ttcgagtccg
     3661 atgcgcgcgg ccacgtcgac gcccgtctcg gctggctcgt catccttggt tcattgccga
     3721 tcgccattct cggattgctg ttccaagacg ccatcgagac atatctgcgg aatctgtacc
     3781 tgacagcagc gatgctggtc ctattcggcc tgatcctggg cgcggccgac cgttacggca
     3841 agcggcgacg ttcccttgac cagatgacct ggcgcgacgg catactgttc gggttcgctc
     3901 aggcgatggc cctcattccg ggggtctcac gctccggcgg aaccatcact gcgggactga
     3961 tcatggggta ttcccgcgag gccgcagccc gctactcgtt tctgctcgcc atcccggcgg
     4021 tcatggcctc aggcttatac cagctctaca aaagctgggg agttgccgga ccggttgacg
     4081 cggcgtctac ggcgttggca acagcggtat ccttcgtggt tggctacgtc gttatcgtcg
     4141 tattcctgcg catcgtcagc agccgaggct actggccgtt cgtgctctat cgcatcgtcc
     4201 ttggcttttc ggtcatgacc ttgctcgggc tcgggcatct aacgccctat tgaacctgcg
     4261 aacccggacg ccctccatca cccgatggag aggaacgcac ctttacctgg aacgattgag
     4321 ggcgcggccc aggtggccgg tgggggcgac gtgttcctgc tggtcgcgta tgtgctgctg
     4381 gcgttgctgt tctcgtttct ctgctcgata gcagaagcgt cgctgctcag catcacccct
     4441 tcttatgttg ccgatctgaa agacaagcag ccggggagag ccgagctgct gaaaaaactc
     4501 cgggaagaca atatcgaccg ttcgcttgct gcgatcctga cggtcaacac cattgcacac
     4561 acggtgggag caattggtgc cggggcgcag gcgacactgg tcttcggtag cgcctggttc
     4621 ggtgttttct ccgccgtcat gactctgctg atcctgttcc tgtcagagat cgtgcccaag
     4681 acgctgggcg cagtctactg gcgtcagctt gcgctcccga ctgccatcta tgtcaatgtg
     4741 ctggtgaaga cgatgtatcc gttgatctgg gtttcggaaa agctgacgca atggatcgcg
     4801 ggcggccaga aatcccagcc gttcagccgc gatgagtttg tcgcaatggc ggggctcggg
     4861 cagcaggagg gacacatcga cgaacgggag agcaagatca tccgcaatct gttcctgttc
     4921 cgttcggtcg ataccggcgc ggtcatgacc ccgcgtgtcg tggtcgccgc ggtccagcag
     4981 gacacgccgg tcgaacaggc actcgaggcg accgagagca cgcctttctc gcggctgccg
     5041 gtctacgaaa ccgatctcga caccgtgacc ggctttgttt tgcgggagga tctccttgtt
     5101 gcgcaaaacg aagggcgtgg gcatgtgccg gtcagggagt tccggcgcga cctcattgcc
     5161 gttgtggaca gcacgccctt gtcccgtctc cttgagacct tgctcgccta ccgccagcat
     5221 atcgccgcag tcgttggtga atacggggag accaaaggcg tcgtcacgct cgaggatgtg
     5281 gttgaaaccc tgcttggtat cgagatcgtg gatgaaggcg acaagattgc ggacatgcag
     5341 cagcttgccc ggcagatgtg ggcacgcagg gccgggcgcc tcggcatcag gccggagcag
     5401 acctgagaca tctcacgtgt gcgaggtcag tcgagagcgt gccagagctg ggttgcgatc
     5461 atcagcagaa acatgactcc gagcaggagc cacttcagtt tctcgttcat ggtattgagt
     5521 aggagtgaat tcaggggggc gggcgcgcga gaaggcccgc acaccaattg tttaagaggc
     5581 cggcgccgat tcgatgcaag tcttggtcaa tgcgtcgaag agcgattgca tcctctcgat
     5641 ctgctcgagg gacatggcgc ggtagatgtc cgacttgcgc gggtcggagg ccagcctgag
     5701 cgcatagccg agcccggtcg cataggcgaa gggcacatat gaggcgtctt gcagtgagac
     5761 gacatggcgt tcaagcaggc ttccctccag actttcctcc acttcaagac gacaagtggc
     5821 cagcaggcgg tatttgtagg aattaccttc cgcttcgacg cggcccaacg cgttcatttc
     5881 ggctagaagt ttctgctcct gcggcgatac gctcaccgaa ggagagggtt cttgccggct
     5941 gtcgcatgcc gcactgagga gaaccgcggc agccatggcg cagcagcccg cgataagacg
     6001 ggccaggcgt cggttcatgg cgtatccttt tctgccgctg ctccggcagg gccagcttcc
     6061 gttggcggcg aaggcaggcg cgaagccagc accttgtcga tccgtttgcc atccatatcg
     6121 acgacttcaa accgccatcc gtcccatgtg gcgatatcgg cggtctgggg aacccgctcg
     6181 agcaggcaca tcaagaggcc gctaagcgtg tggtagcggc cgcgctcttc ttcaggaaca
     6241 gacttcagct ccagcttgtc cttgagttcg ggaatcggaa tcatgccatc aagcagccat
     6301 gacccgtcct cgcgctggac cgcccaggcg tcgtccagat tgctaggagt gaactcgccg
     6361 gtgaccgcct ccagcacatc ctgcagtgtc accagaccct gtacttctcc gtactcatca
     6421 atcaccagga ccatctgggt ctgcgatgcg cggaagattt caagcagcgc catgccggtc
     6481 atcgcttccg gcacgaacac ggcaggctgc gcgctggccg ccaggtccgg catgccaccc
     6541 atgagggttt ggctcagaac ctgctttgcg ctgaggatgc cgatgacgtc acgcaggccc
     6601 ccgcggcaaa ccggaaagcg cgaatgttcg gaagcggtga tgcgcttgag gttctcttca
     6661 atcggcaggt tgacgtcgag gtagacgatg tcgaagcgcg ggatcatgag cgagccgacc
     6721 aagcgttcgt caagccggaa cacgttgcgc accattgcat gctcgtggcg ttctatcacg
     6781 ccggcttccg agccttcggt caggagggcg tgaatctcct cctccgtcac accctggtcc
     6841 tgttgctcgc gcttgccaag gaggcgcagc gtcgtgtcgg tcgacgccga cagcagccgc
     6901 acgaacgggc gggttagccg ggacagcccg ttcatcggcc gtgcaacgag caaggcaatg
     6961 ccttccggat tgaattgccc catgcgcttg ggcacgagct cgccaaacac gatggcaaag
     7021 tacgtgatga tcacgacgac agccgtcgtc gcagcgatct ggctggtcgc ctgggcgatg
     7081 ccgagagact ggaaccatgt cgcaagcgga cctgcaagcg cgctctctcc gacgatgccg
     7141 ctcagcacac cgatagacgt aatgccgatc tggattgttg agagaaaacg ggtcggctcg
     7201 tcatgtaagg caaccgcgac agcggcgggc cggctgccgt cctcggcgag ccgggcaagg
     7261 cgggcgcggc gcgccgtcac gagcgcaatc tcggacatgg cgaaaacgcc attgagcagg
     7321 atgaggccaa gaagtatcag gacttccatc acgtgcctcc agtgcgcaag gcgaatgtgc
     7381 ccaattgcag gctgctgccg ccgcgaatca acgcaattaa cctgcaggaa aggaatgtat
     7441 tcgcacccgg agggggcgga aaggaaagct taaagcggga agacgcgcgc ttgggcgcag
     7501 gagaagggca acttcgacaa ctcgaacagt ccatggtggt ccaggcaagc ctggaagatc
     7561 ctcactagag tttgggaagc gatatcctaa gacggcgcat tgacttcgtc aaatgccatc
     7621 gagtcctgtt ctttccatcc tagagctata tctactcggg gctagcgagc gtcttcttgc
     7681 cagggaatac attaatcgtc gttgcggaca aaagttcata acctattgag gatcataatt
     7741 accgggaaca actagttgcc cgcaagttac gttgcccgtt cgtctgcttt taaacataac
     7801 cggaagaggt tgcgcgtggc tgccccggca ctcgggcagc ccaatggaag tcatcattcg
     7861 ggcagccctt gcattgccac aagcggagcc ctcccgcccc gcgctaagct ggccgctgcg
     7921 ttgaaaagcc gacccagccg cccaacaaga tgacgcttcc caagaccgcc attgcagcca
     7981 gcgcgattgc gctcccctgc cttgctctgc ccctgccggc cctcgcccag gcggagaccc
     8041 tcgcgcccat cgtggtcacc gccactgccc caggcgccga cacgcgcgat ctgcccctgt
     8101 cgatcgaccg cctcgaccag gatgacatcc aggtcgggaa cccgatggtc aacctgtcgg
     8161 agacgctcaa ccgcgtgccc ggcatcgtgg tgcgcaatcg ccagaactat gcgcaggacc
     8221 tgcagatttc ctcgcgcggc ttcggcgcgc gcgcaacctt cggagtgcgc ggcgtgcggc
     8281 tgtatgtgga cggcattccg gccacgatgc cggacggcca ggggcagacc tcgaacttcg
     8341 acctcgcgtc ggctgctcac atcgacgtac tgcgcggccc gttctctgcg ctctacggca
     8401 actcctcggg cggcgtggtc tcgatctaca ccgaggacgg acaacctggc ctcgcgctgt
     8461 ctccgttcgc gcaattcggc agctggaaca cgcgccgcta cggcatcaag agcgccggcg
     8521 agcacggcat gctcaatcat gtattgagca tcaaccgctt cgagaccgat ggctatcgcg
     8581 cccacagcgc ggcacgtcgc gatatcgcca acgcgaagct gcgcctcgtc ccgggcggcg
     8641 acacgatctg gacgctggtc gccaaccgcg tttcgatgcc ggaggtgctc gacccgctcg
     8701 gcctgccgcg cgaacagatg ctggcggcgc cgcgcagcgt gcatccgaac gcactgctgt
     8761 tcaacacgcg caagtcggtg cgtcagcagc agctgggcat gaacgccgag catttcctga
     8821 acgcgcagga cagcctgcac ctgatggcct atggcggcca gcgcgatgtg ctgcagtttc
     8881 tcgccattcc cccggcacct caggcggcgc ccacgcactc gggcggcgtg gtggatctgg
     8941 gccgcgattt cggcgggatc gacctgcgct gggctcgcga agccgcagcc ggctggctat
     9001 ggacagccgg gctcagcgtc gagacggtcg aggaagaccg gcgcggctac gagaacttca
     9061 tcggcgatac gccgggcgtg cgcggcgccc tgcgccgcaa cgagcgcaac accatcacca
     9121 gcatcgagca gtatgcacag gcgcaatggg agccgacgca acaatggctg ctgctggccg
     9181 gcgtgcgcca cagcatggtg cgcgtgcgca cgcgcgaccg ctacgtcgcc cccggcaatc
     9241 cggacgacag cggcgccatc cgctaccgcg ccaccaatcc ggtgctgggc gccacctggc
     9301 gcgccaccgc cgacctgaac gtctacgcgt cctacgggcg cggcttcgaa acgccgaccc
     9361 tgaacgagct gtcgtatcgc acgagcggga atggcgccgc gggcttcaac ttcagcctgc
     9421 gtccggccac cagcagcaat atcgaggtag gcatcaaggc cgccgtggcg caacactggc
     9481 ggctgaacct tgcagccttt catatcgaca cgcgcaacga actgacggta ctttccagcg
     9541 ccggcggccg cgcagtgttc cagaacgccg catccacccg ccggcgcggc ctcgaacttg
     9601 gcgtcgaagg ccgctgggac aacggcctcg gcatcctgct gtcggcgagc ctgctcgatg
     9661 cgcgctatgc cgaccgcaat ggcgcaacca ccttcgccgg caaccgcctg cccggcgtgc
     9721 ccgcgcgcac cggcttcgcc gagctgtcct ggcgcgcccc gcagggcggc ttctccaccg
     9781 ccctcgaact ccagcacgtg ggccgagtct acgtcaacga cgccaacagc gaagccgccg
     9841 atgcctacac gatcgccaat gcccgcatcg ggttcgagca gcagatcggc gggtggaccc
     9901 tgcgcgagtt cctccgcgtg gacaacctgg ccaaccgcag gcatgtgggg tcggtaatcg
     9961 tcaacgaggg caacgggcgc ttctacgagc ctggcccggg ccgacatgtg ctggtcggcg
    10021 tgacggccag ccatgcatgg aagtagccgc aataaaaacg ctcgggacgc ctcgaacgcg
    10081 tcatattccg gtcattccgt acctttacaa tttacgacgt ttccccgaca atgcgcgctt
    10141 catcaattgc ggagtccatt cgtgggcact tgttcgagtg acagtagtac ctgtcgatct
    10201 tccggcagga cgttctcggc gaaatagcgc gcaagtctgt tcttgctgct gtctcgccta
    10261 gagcgcgagg cggcggcgac cgatcccggc atccagccgg atcccagctt cccctgcata
    10321 caatctggtg caaccccgtc gtggccgtcc attgctgtga aatgggcggc acctcgtccg
    10381 ttcggcatgc gtgcatgcgc acatgtcgca gagcggactg aacggggcgg cgccacgcgt
    10441 tccgttcgcg gaacgcctgt cccgatccag cgcttgcatc ggtcttcgcc tctctcagaa
    10501 ggaggcaatg ccatgcgttt cacacaagcg ttcaaagcca tcccgcccgt cgcgttggcc
    10561 accctgtgca tgcttgcggc cagcgccgcg caggcgggcc gtccgatgat taccgacgac
    10621 gcggccatcc tcgaagccaa cgcctgccag ctcgagacct ggtaccaggc cacccgcagc
    10681 gccggtaatg aatggtgggc cctgcccgcc tgcaacgtgg gcggctacgc ggaagtggcc
    10741 atcggcggcg cacgcctgcg cgagcctgac ggccgcacca ccgtcgcggt gatccaggca
    10801 aaggcgctgc tgcgtgctta cgacgaaacg cgccccggct tcggcatggt gctcggcacc
    10861 gagcgcgatc gcggggtgca ccgcggttcg gccggaaatc ccttcatcgc cggcattgcc
    10921 agtgtcccgc tgcgcggcga agccatgctc ctgcatgcca acctcggctg gcagcgcgtc
    10981 cgcgacgatt tcgccggcac ccgcgaaaat tccgccacct gggcgctggc gctcgacagc
    11041 gaagtcgccg cccgcacccg cgtatcgctc gaatcctatg gccagtcgcg cgagcgcgcg
    11101 atggcgcagt tcggcgtggc ctacgatctc gccccgggcc gggtgcagat cgacggcagc
    11161 gtcggcagcc gcatcgcgcg cggcgcgccg gaccgctacg ccaccatcgg actggtcttc
    11221 ttcaccgatc ctctcttccg ctgaacaagc agaaccgacc agacaaacac tgatgacatg
    11281 ccggcagcga tgctgcccgg cgggaggaag aaatggaacg cgaagaactc atcgtctata
    11341 tccagtcgct gctggaagcc caggacgcgc agaccatccg tgcgctgatg aaccgggtat
    11401 cgatggcaga cctggccgag gccttgtcga acgtgccggt cgaagacgcc gtgcgcttct
    11461 ggaacatgct ggaccaggaa gaacggctcg agctgttcgt ctatttcccg cacgggatcc
    11521 aggatgcgat cctcgcgcag ctgccacgcc ctgccctggt cgccctgttc gatcagctgc
    11581 cgtcggatga ccgtgccgac atctataagc gcatggacga cgagatgcgc gagaagttga
    11641 tgccggcgct ggccaaggtc gagcgcgatg acatcctgaa gctcgccgcc tacgaggaag
    11701 ggacggtcgg ctccatcacc acgtcggact acgccaccgt gcaggaagac atgaccgtgg
    11761 ccgaagcctt gcgcgtgatc cgcgcgggcg cgccggacaa ggaaaccatt tacgtcgtct
    11821 acgtgctgga cgagctgcgc cgtctggtcg gcaccgtgtc gctgcgcgaa ctggtgctgg
    11881 agccggagtc gacccgcttg ggcgacctga tgcaccgtga gccgatctat gccgaggccg
    11941 acgcgccgcg cgaggaagcc gcgcgcctga tctcccgcta tgacctgctg gccgtgccgg
    12001 tggtcgatgc gggccgacgc atgatcggca tcgtcaccgt cgatgacgcg atggccgtcg
    12061 cccaggaaga aaccactgaa gacttccaca agtccggcgg catggccgcg cccgaaggca
    12121 tgtcggtgcg ctcggcctcg gtgttccagc tgtatcg
//
LOCUS       DATLGF010000057         3416 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_1333,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000057 DATLGF010000000
VERSION     DATLGF010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VM406_01860"
     CDS             complement(<1..989)
                     /locus_tag="VM406_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HVL74735.1"
                     /translation="MRFRNQLLVLVLSVVIPAFLAAILAVWYVFSEEQQGQEKGMLEA
                     TRTFSLLVGKELQAREAALLTLRNSAALAKGDLAEFYSIARRLAPTPETAIFLTDRSD
                     RQILNTRAPFGAALPPGRSGNFSALLEGDGSGRTLVSDVFLAPVGKRFDFSIQVPVYD
                     AGGLRYFLGMGINASAMQPLLDLQRLPANWLAVILDRQGVVVSRSRNPEQYVGKAATD
                     EFRARLSAAREGVFPSVTLDGVPVKTFFSRVAFSDWTVIVSIPEAELRSAPLRAAAFL
                     AAIMAILLGVAFFVAQRIAASVNTRMEQLGVAAEKLGMGEEVAYRPQGLVEIDT"
     gene            complement(1123..2049)
                     /locus_tag="VM406_01865"
     CDS             complement(1123..2049)
                     /locus_tag="VM406_01865"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL74736.1"
                     /translation="MNLLVALEALMAERHVTRAAERVAMSQPAMSRALGRLRDTFHDP
                     LLVRAGSEMVPTARGRALAQQVGRVLDEVRNMLTEDSFEPASFTGRFKLLTVDYASLT
                     SLPAMLGALLGEAPEMRMEVADAGNDWADRLQAGEADLVLGVVGAAPAGIFQRAVLED
                     RFACIMRRGHPLAGRPLDLDSFLAQRHVLISSPGRERSAVDMALERLGRPPRNITLRL
                     PHFMASTAIIAATDLVMTLPQRIAAYMARHEALELRDPPVELEGFTLRLLWHQRSHDD
                     PAHRWMRERGAKAIQNAARLPAVRSRAGKSQE"
     gene            2166..3038
                     /locus_tag="VM406_01870"
     CDS             2166..3038
                     /locus_tag="VM406_01870"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HVL74737.1"
                     /translation="MYAVIGVSGNTGSAVARALLDLGRKVRAVVRRPEAGDEIRKQGG
                     EIAVADLGDAKALAAALTGVDGAYIMTPPLLDVQDPDGTKRKMIATIRAAAEQAGAPP
                     LVVLSSEGAHHSSGTGPVVSLHALEQSLRDYPGRLTFLRAPYFLDNWMEAIAPATEQG
                     VFPSFMAPGVGIDMATTEEIGRTAAQLLLETPGHHRVVELRSARLYSAQDVARELGQL
                     LGKPLEVVYPPRSEWNGILEGAGLSRIGAAQMAELFDCINSGRIGFSGEGEYRTAHEG
                     PAAMFRRALGVNTV"
     gene            complement(3169..>3416)
                     /locus_tag="VM406_01875"
     CDS             complement(3169..>3416)
                     /locus_tag="VM406_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HVL74738.1"
                     /translation="TSLSCTKRMYPMHYPFPPQDVVEFFFTYYGPTVRALAALDPDKQ
                     RTLREELVQLWAQNNRATDGTSTHIESEYLEVDGVRA"
BASE COUNT          625 a         1091 c         1120 g          580 t
ORIGIN      
        1 gtgtcgatct cgaccagtcc ctgtggccgg tatgcgactt cctcgcccat tcccagtttt
       61 tctgctgcca cgcccagttg ctccatgcgc gtgttcacac ttgccgcaat ccgttgtgca
      121 acgaagaacg cgacgccgag cagaatcgcc atgatggcgg caaggaaggc cgcggcgcgc
      181 aagggcgcac tgcgcaactc cgcttcgggg atgctcacga tgacggtcca gtcggagaag
      241 gcaacacggc tgaagaaggt tttgaccgga acaccgtcca gggtcacgct ggggaacacc
      301 ccttcgcggg cggctgacag ccgtgcccgg aactcgtccg tcgcagcctt gccgacgtat
      361 tgttccggat tcctcgagcg cgacacgacc acgccctgcc ggtcgagaat gacggccagc
      421 cagtttgcag gcaggcgttg aaggtccagc agaggctgca tggcggaggc attgattccc
      481 atcccgagga agtaacgaag cccgccggcg tcgtagaccg gcacctgtat cgagaagtcg
      541 aaacgttttc cgaccggagc aagaaagacg tccgatacca gggtgcgacc ggaaccatct
      601 ccttccagca gcgcactgaa atttccggag cggcccggcg gcagcgccgc gccaaacggt
      661 gcccgcgtgt tcagaatttg ccggtcgctg cggtcggtga ggaagatggc ggtttcgggt
      721 gttggcgcaa gacgcctggc gatcgaatag aactccgcca gatcgccctt ggcaagcgct
      781 gccgaattgc ggagtgtgag cagcgcggct tctctggcct gcagctcctt gccgacgagg
      841 agggaaaagg tccgtgtcgc ttccagcatt cccttctctt gcccctgctg ctcttcgctg
      901 aagacgtacc agaccgcgag gatcgcagcc agaaaggccg ggattacgac agacagcaca
      961 aggaccagca gttgattgcg aaagcgcatg gggcggttcg aggtaggcgg gccgacgcgg
     1021 gagggagcgc cccgggctgt catggagcga cggcttgccg actatacatc atgtggatgc
     1081 gatggacaca agcttggcgg gtgggcggta caccgctttg gctcattcct gcgattttcc
     1141 cgccctgctt cggacggcag gcagtcgcgc ggcattctgt atcgctttgg ctccgcgttc
     1201 gcgcatccag cgatgagcgg ggtcgtcgtg gctgcgttga tgccagagca gacgcagcgt
     1261 gaatccctcc agttccaccg gggggtcacg cagctccagg gcctcatggc gggccatgta
     1321 tgcggcgatc cgctgcggca gggtcatcac aagatcggtc gctgcaatga ttgcggtcga
     1381 cgccatgaag tgcggcaggc gcagggtgat gttgcgcggc ggacgaccaa gccgctccag
     1441 cgccatatcc acggcactgc gttcgcgtcc cggcgaactg atcagcacat ggcgctgcgc
     1501 gaggaaacta tcgaggtcca gcggccgtcc cgccaacgga tgtccgcgcc gcatgatgca
     1561 ggcgaaccgg tcttcgagca ccgcacgctg gaagatgccg gcgggcgcag cgccgactac
     1621 cccgagcacc aggtcggcct caccggcctg gaggcggtcc gcccagtcgt tgccggcgtc
     1681 tgccacctcc atccgcatct ccggcgcctc gcccagcagc gctccaagca tggccggcag
     1741 cgaggtcagg ctggcgtagt cgaccgtcag caacttgaag cgcccggtaa agctcgccgg
     1801 ctcgaacgaa tcctccgtca gcatgttgcg gacttcgtcc agcacgcgac cgacctgctg
     1861 cgccagagcc cggcctctcg ccgtgggcac catctcggaa cctgctcgca ccagcaacgg
     1921 gtcgtgaaag gtatcgcgca gccggccgag agcacggctc atcgccggct ggctcatggc
     1981 cacccgctcc gcggcccggg taacgtggcg ctctgccatc aatgcctcca gggccacgag
     2041 caggttcaaa tccagcgcgc gaagattcgc ttcatgcatg acggctatcc caaatatgca
     2101 ttggacgcat cttaacccgg tacttagcat gaaggctcat cgtcgctgaa accaggagaa
     2161 atgctatgta cgcagtcatt ggagtatctg gaaacaccgg ctcggccgtg gcccgggccc
     2221 tgctcgattt gggcaggaaa gtacgcgcgg tcgtccgccg gcccgaggcg ggcgacgaga
     2281 tccgcaagca aggtggtgaa atcgccgtgg cggatctggg ggatgccaag gccctcgccg
     2341 cggcgctcac gggcgtcgac ggcgcctaca tcatgacccc gccgctcctc gatgtgcagg
     2401 acccggacgg caccaaacgg aagatgatcg ccaccatccg cgcggccgcc gaacaggccg
     2461 gcgccccgcc gcttgtcgtg ctctcctccg agggcgccca ccattcttcg ggcaccgggc
     2521 cggtggtgag tttgcacgcg ctggagcagt cgctgcgcga ctatccgggc aggctgacct
     2581 tcctgcgggc accctacttc ctggataact ggatggaggc aattgcaccg gcgaccgaac
     2641 agggtgtgtt cccgtcgttc atggcaccgg gggtcggcat cgacatggcg accaccgaag
     2701 agatcggccg gacggccgca cagctgctgc tggaaacgcc cggccaccat cgcgtcgtcg
     2761 aactgcgcag cgcccgcctg tacagcgcgc aggacgtggc gcgcgagctc ggccagctat
     2821 tgggcaagcc gcttgaagtg gtgtacccgc cgcgcagcga gtggaacggc attcttgagg
     2881 gtgccgggct ctcgcgtatc ggcgcggcgc agatggcgga gctgttcgac tgcatcaaca
     2941 gcggccggat cggcttctcc ggcgaaggcg agtaccggac cgcccatgaa gggcctgcgg
     3001 cgatgttcag acgggcgctt ggagtgaata ccgtgtgagg cggagtgaac ggggcagact
     3061 gcattactcg cgcggtgttc ctgggaaggg gacagggctc tgcaaacgca gtctgctgcc
     3121 tgtcacgcca cccttgcacg atgaatgcgg ggtgcagcaa tccactcctt aagcacgcac
     3181 cccgtccacc tccagatact cggactcgat gtgggtcgag gtaccgtccg tcgcacggtt
     3241 gttctgcgcc catagctgca ccaattcttc gcgcaaggtc cgctgcttgt ccgggtcgag
     3301 cgccgcgagg gcgcgtacgg tcgggccgta ataggtgaag aagaactcga ccacgtcttg
     3361 cggaggaaac ggataatgca tcggatacat gcgctttgtg cagctgagcg aagtcg
//
LOCUS       DATLGF010000058         3431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_207194, whole genome shotgun sequence.
ACCESSION   DATLGF010000058 DATLGF010000000
VERSION     DATLGF010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207194"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /gene="miaA"
                     /locus_tag="VM406_01880"
     CDS             complement(<1..786)
                     /gene="miaA"
                     /locus_tag="VM406_01880"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HVL74739.1"
                     /translation="MKPLVVAILGPTASGKTAAALALAEALPCEIVSMDSALVYREMD
                     VGTAKPTAAERAAVPHHLIDIIDPADAYSAMQFRGDALRLVEEIRARGRLPLLVGGTM
                     LYYKALRDGLDALPEADPALRARLDEEAERFGVPALHARLAELDPLTAARLKPNDSQR
                     IQRALEIQMLTGRPMSALLAQAPKPALPFDLLPISLEPSDRTVLHERIGRRFDAMLAA
                     PLSLLDEVEGLRARGDLHLGLPSMRCVGYRQAWEYLDGLYDRAA"
     gene            complement(783..1991)
                     /locus_tag="VM406_01885"
     CDS             complement(783..1991)
                     /locus_tag="VM406_01885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HVL74740.1"
                     /translation="MEARPLAPYAHAPLLLFIASFLMLQPLSTDLYLASLPGLATYFQ
                     VPAATVQLTLSLFVLGFGAAQLVVGPLSDRFGRRPVALGGLALYVFASLLCSIATSVG
                     LLIAARFLQALGCCAAVVISRAVVRDAYAPEHGARVVARASTWLSLAPIFGPILGSYL
                     QVAFGWRAAFVALATLSAGLLMLSIARMPETNAHKNAEATRVAGLLGNYRHVLASRAF
                     WAHALPGALSYGAIFAFISGSSFVLIRVLGVPTEWFGYCFALGVVGYMLGTLACRRLL
                     AALGAQRTLRLGSLVSLASGAGFVLAVLAGVAHWSLVVAAMFVTMAAHGINFPVAQAG
                     AVAPFPKQAGTAAGLMGALYMGVAFLVGTTVGATHDGTLLPLALVSCALGLCIFASAR
                     AIRLAAEPAR"
     gene            complement(1994..>3431)
                     /gene="mutL"
                     /locus_tag="VM406_01890"
     CDS             complement(1994..>3431)
                     /gene="mutL"
                     /locus_tag="VM406_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HVL74741.1"
                     /translation="PGAVPQPSSGAPGTTVDVRELYFNTPARRKFLKSEQTEFGHCAE
                     VVRRIALSRPDVAFSLTHNGKTVDHWNLAEFARRSAQVLGGEFATARLPLDEAAGPLR
                     LHGFVGLPTASKARADAQYFYVNGRFVRDKLLTHAVRAAYQDVLHGDRYPSYVLALEL
                     DPALVDVNVHPSKIEVRFRDSRAVHQFVFHAVARVLAHTAPGATDDAIAPVAAPSRAI
                     PWIRTPQQVSFGAQFHAPPPAGVAQTLADYGALFQSASVDVPSAAAQSPQRYNASAPR
                     ADEQDAQDEFPLGFALAQLHGIYILAQNRKGLVLVDMHAAHERILYEQLKCALDGDAM
                     QVQPLLIPVSFHADAVEVGTAEENRDALTALGFDISALSPTTLAVRAVPALLKNADAQ
                     ALARDVLRDVREFGGSRVLIERRNELLGTLACHTAVRANRSLTLPEMNALLRQMEATE
                     RADQCNHGRPTWVQLALPDLDRLFLRGQ"
BASE COUNT          580 a         1160 c         1253 g          438 t
ORIGIN      
        1 ggccgcgcgg tcatagagtc cgtcgaggta ttcccaggcc tgacgatagc cgacgcagcg
       61 catcgacggc aggccgagat gcaggtcgcc gcgcgcgcgc agaccttcga cctcgtcgag
      121 cagcgaaagc ggggcggcaa gcatcgcatc gaagcgccgc ccgatgcgtt cgtgcaggac
      181 cgtgcggtcc gagggttcca gcgagatcgg cagcaggtcg aacggcagcg ccggcttcgg
      241 cgcctgcgcg agcagggccg acatcggccg cccggtcagc atctggatct ccagcgcgcg
      301 ctggatgcgc tggctgtcat tcggcttcag ccgggcggca gtgagcggat cgagctcggc
      361 cagccgcgcg tgcagcgcgg gcacgccgaa gcgttccgcc tcttcgtcga gccgcgcgcg
      421 cagcgccgga tcagcctcgg gcagcgcatc cagtccgtcg cgcaaggcct tgtaatagag
      481 catcgtgccg ccgacgagca gcggcaggcg cccgcgcgcg cgaatctcct cgaccaggcg
      541 cagcgcatcg ccgcgaaact gcatcgccga ataggcgtcg gccggatcga tgatgtcgat
      601 caggtgatgc gggacggcgg cacgttccgc ggcggtcggc ttggcggtgc cgacatccat
      661 ctcccggtag accagggccg agtccatcga cacgatctcg cagggcaacg cctcggcgag
      721 cgccagcgcg gcggcggtct tgccggatgc ggtggggccg aggatggcga cgaccagcgg
      781 tttcatcggg ccggctccgc cgccaggcgg attgcgcgcg cgctggcgaa gatgcacagg
      841 ccgagcgcgc acgacaccag ggccagcggc agcaacgtgc cgtcgtgcgt cgcgccgacc
      901 gtggtgccga cgaggaaggc gacgcccatg tagagcgcgc ccatcagtcc ggcggcggtg
      961 ccggcctgtt tcgggaatgg cgcaaccgcg ccggcctggg caaccgggaa attgatcccg
     1021 tgcgcggcca tcgtcacgaa catcgcagct accaccagcg accagtgcgc cacgccggcc
     1081 agcacggcca gcacgaagcc ggcgccgctg gcaagcgaaa ccaggctgcc caggcgcagc
     1141 gtgcgctggg cgccgagcgc ggcgagcagt cgccggcagg ccagcgtccc cagcatgtag
     1201 ccgaccacgc cgagcgcaaa acagtagccg aaccactcgg tgggcacgcc gagcacgcgg
     1261 atcagcacga aggatgagcc cgagatgaat gcgaagatcg cgccatacga cagcgccccg
     1321 ggaagcgcgt gcgcccagaa cgcccgactg gcaagcacat gccggtagtt gccgagcagg
     1381 ccggcgacgc gggtcgcctc ggcattcttg tgtgcgttgg tttccggcat gcgcgcgatc
     1441 gacagcatca gcaggccggc cgacagcgtc gccagcgcga cgaaggcggc gcgccagccg
     1501 aatgcgacct gcagatagga cccgaggatg gggccgaaga tcggcgccag cgacagccag
     1561 gtgctcgccc gcgcaaccac gcgcgcccca tgttcgggcg cataggcgtc gcgcacgact
     1621 gcgcgcgaga tcaccaccgc cgcacagcag cccagcgcct gcaggaagcg cgccgcgatc
     1681 agcaggccga ccgaggtcgc gatgctgcac aagaggctgg cgaacacata cagcgccagt
     1741 ccgccgagcg cgaccgggcg gcggccgaag cggtcggaca gcgggccgac caccagctgg
     1801 gctgcgccga agcccagcac gaacagcgac agcgtcagct gcacggtggc ggccgggacc
     1861 tggaagtaag ttgcgagccc gggcagggag gcgaggtaga gatcggtcga cagcggctgc
     1921 agcatcagga acgaggcgat gaacagcaac agcggggcgt gcgcgtaggg cgcgagcggc
     1981 cgggcttcca tgattactgt ccgcgcagga acagcctgtc gaggtccggc agcgccagct
     2041 ggacccaggt cggccgtccg tggttgcact ggtccgcgcg ttcggttgcc tccatctggc
     2101 gcagcagtgc attcatctca ggcagcgtca ggctgcggtt cgcgcgcacg gcggtgtggc
     2161 aggcgagcgt gccgagcagt tcgttgcgac gctcgatcag cacccgcgag cccccgaact
     2221 cgcgcacgtc gcgcagtacg tcgcgcgcca gcgcctgcgc atcggcgttc ttcagcagcg
     2281 ccggcaccgc gcgcacggcc agcgtggtcg gggagagtgc gctgatgtcg aaaccgaggg
     2341 cggtcagcgc atcgcggttc tcctcggcgg tgccgacctc gaccgcatcg gcatggaagc
     2401 tgaccggaat cagcagcggc tggacctgca tcgcatctcc gtccagcgcg cacttgagct
     2461 gctcatagag gatgcgctca tgggctgcat gcatgtcgac gagcaccagc cccttgcggt
     2521 tctgcgcgag gatgtagatg ccgtgcagct gcgcgagtgc gaagccgagc gggaattcgt
     2581 cctgcgcgtc ctgctcgtcg gcacgcggcg cgctcgcgtt gtaccgttgc ggcgattgcg
     2641 cggcggcgga cggcacgtcg actgaagccg actggaacag tgcgccgtaa tcggccagcg
     2701 tctgcgcgac gccagccggc ggcggcgcat ggaactgcgc gccgaatgaa acctgctgcg
     2761 gcgtgcggat ccacggaatc gcgcgcgacg gcgccgccac cggggcgatt gcgtcatcgg
     2821 ttgcgccggg tgccgtgtgc gcgagcacgc gggcgaccgc atggaacacg aactggtgca
     2881 ccgcgcggct gtcgcgaaac cgcacctcga tcttggacgg atgcacgttg acgtcgacca
     2941 gggccgggtc gagctcgagc gccagcacgt aggacgggta gcggtcgcca tgcagcacgt
     3001 cctggtaggc ggcgcgcacc gcgtgggtca gcagcttgtc gcgcacgaag cgcccgttca
     3061 catagaaata ctgggcgtcg gcgcgtgcct tggacgcggt cggcagcccg acgaagccgt
     3121 gcaggcgcaa cggccccgcg gcctcgtcca gaggcaggcg cgcggtggcg aactcgccgc
     3181 ccagcacctg cgcgctgcgc cgcgcgaatt cggcaagatt ccagtgatcg acggtcttgc
     3241 cgttatgggt gagcgagaac gcgacatccg ggcgcgacag cgcaatgcgg cgcacgacct
     3301 cggcgcagtg gccgaactcg gtctgctcgg atttcaggaa cttgcgccgc gccggcgtgt
     3361 tgaagtacag ctcgcgcacg tcgacggtgg tgccgggcgc gcccgaggac ggctgcggga
     3421 cggcgccggg c
//
LOCUS       DATLGF010000059         2244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_207216, whole genome shotgun sequence.
ACCESSION   DATLGF010000059 DATLGF010000000
VERSION     DATLGF010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207216"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VM406_01895"
     CDS             complement(<1..823)
                     /locus_tag="VM406_01895"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVL74742.1"
                     /translation="MATIEHVIEVNAAPDAVYDCLVRFDAYPSFVQDVRQVRRVGALR
                     LEWQSGHGEQARSWEAEIVQQIPGRRVVLRTMRGPRYEVTAELGSMAAQDGGRGTAVP
                     RTRLRLLIEFDEHEQILPEHGDVVAALRARSEQDLARFKKHVEHHVPAGVHVAALEPA
                     RTRPVELDAGAPVGKTIGQTAAEREESMRSQPHWQQPAEPHAGEHAGERHGHGLRRMT
                     PSLPDLWNLWQQPFRMMRSFSRAVHGFEQVFESVRTGGRREETLPKAWTPTVETAQ"
     gene            1162..>2244
                     /locus_tag="VM406_01900"
     CDS             1162..>2244
                     /locus_tag="VM406_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HVL74743.1"
                     /translation="MSESTRAAEAAAARQPAEPAHSDTRVLRDLWSVVSRFRARIVAA
                     LVFLVLAKIAVVSVPLVLKRIVDDLSRPEQLVALPIILLAGYAILRFASTLFNELRDL
                     AFSRVTQHTVATYAQKVFSHLHELSARFHAQRRLGGLLPDIDRGTSGIAFLLSVGLFT
                     IVPTLLEIGLVVAILTSRYSLYFTLILAVTFLVYSGFTIVFTARRAIFQRRVNRLDSS
                     AKSLLADSLMNYDTVKYFTNERQESNRFAGVMDHWTDAAVANQKALFILHVGQSAIIA
                     GGVAAVMLLAGNGVMTGAMTVGDLILINAYVIQVCLPLNALGFVYREARDALVNAEKL
                     FALLREKPEVDEKPGMPQLEVSRGEVV"
BASE COUNT          355 a          778 c          720 g          391 t
ORIGIN      
        1 gctgggccgt ttccaccgtg ggcgtccagg ccttgggcag cgtctcctct cgccgtccgc
       61 cggtgcgcac gctctcgaac acctgctcga aaccatgcac ggcgcgcgag aagctgcgca
      121 tcatccggaa cggctgctgc cacaggttcc acagatcggg aaggctgggc gtcatccgtc
      181 gcagcccgtg gccatggcgt tcccccgcgt gttcgcccgc atgcggctcc gccggctgct
      241 gccagtgggg ctggctgcgc atcgactcct cgcgctcggc cgcggtctgt ccgatggtct
      301 tgccgaccgg cgccccggcg tcgagctcga ccggccgggt gcgcgccggc tctagcgccg
      361 ccacgtgcac gccggccgga acatggtgct ccacgtgttt cttgaaacgc gccaggtcct
      421 gttccgagcg cgcgcgcagc gcggccacga catcgccgtg ctcgggaagg atctgctcgt
      481 gttcgtcgaa ctcgatgagc agccgcagcc gggtgcgcgg cacggccgtg ccgcgcccgc
      541 catcctgcgc cgccatgctc ccgagttcgg ccgttacctc gtagcgcgga ccgcgcatgg
      601 tgcgcagcac cacgcgccgg cccggaatct gctgcacgat ctcggcctcc cagctgcgcg
      661 cctgctcgcc atggccgctc tgccattcga ggcgcagtgc gcccacccgc cgcacctggc
      721 gaacgtcctg cacgaagctc gggtacgcgt cgaaacgcac gaggcagtcg tacaccgcgt
      781 ccggcgcagc attgacctcg atcacgtgtt cgattgttgc catggccact ctcctcgctg
      841 ttttccgcac cactgaccgt gtggaatgga gtgtgctacc ggaatcacgg cgggaccgga
      901 agctcacgcg gcgatggacg gatcgcaatt tccataccgg cccgtgcggg ctgcgccagc
      961 cagggcccgt gcggcggcat gcgcctggaa cgcgttcgcg cgcagcccgg cttggccctc
     1021 agaacggaag cgggaaatga agatcggaca ggcatgcagc gcctcagcgc gaaggtgtgc
     1081 gccgacaaga ttgcacgggc cgggtaaaga ttacagaagc gcatcagcac gccggtccga
     1141 acggaaagga gaagcagcaa gttgagcgag tccacacgtg ccgccgaagc cgcggcggca
     1201 aggcagccgg ccgaaccggc ccattccgat acccgcgtgc tgcgcgacct gtggagcgtc
     1261 gtctcgcgtt ttcgcgcccg tatcgtcgct gcgctggtct tcctcgtgct ggcgaagatc
     1321 gcagtggttt ccgtgccgct cgtgctcaag cgcatcgtcg atgatctttc ccggccggaa
     1381 cagctggttg cgctgccgat catcctgctg gccggctatg cgatcctgcg cttcgcgagc
     1441 acactgttca acgaattgcg cgaccttgcc ttctcgcgcg tgacccagca cacggtcgcc
     1501 acctatgcgc agaaggtgtt ttcccacctg catgaactga gcgcgcgctt ccatgcacag
     1561 cgccgcctgg gcggcctgct gcccgacatc gaccgcggta ccagcgggat cgccttcctg
     1621 ctcagcgtcg gcttgtttac gatcgtgccg acgctgctcg agatcggact cgtggtggcc
     1681 attctcacca gccgctacag tctctacttc acgttgatcc tggccgtgac ctttctcgtc
     1741 tacagcggct tcacgatcgt gttcactgcc cgccgggcga tcttccagcg ccgcgtcaac
     1801 cggctggatt ccagcgccaa gagcctgctg gccgacagcc tgatgaacta cgacacggtg
     1861 aagtacttca cgaacgagcg gcaggaatcg aatcgcttcg ccggcgtgat ggatcactgg
     1921 accgacgcgg cggtggcgaa ccagaaagcc ctgttcatac tgcacgtcgg gcaaagcgcg
     1981 atcatcgccg gcggcgtggc cgcggtgatg ctgctcgccg gaaacggcgt gatgaccggc
     2041 gcgatgacgg tgggcgacct catcctgatc aacgcctacg tgatccaggt ctgcctgccg
     2101 ctcaatgccc tcggcttcgt ctaccgcgag gcgcgcgatg cgctggtcaa cgcggaaaag
     2161 ctgttcgcgc tgctgcgcga aaaaccggag gtcgacgaaa agcccggcat gccgcagctc
     2221 gaggtgagcc gcggcgaagt ggtg
//
LOCUS       DATLGF010000060         3477 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_19066,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000060 DATLGF010000000
VERSION     DATLGF010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19066"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VM406_01905"
     CDS             <1..447
                     /locus_tag="VM406_01905"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVL74744.1"
                     /translation="LHIPYKGANEAQIDVATGLVDFFFANLPSALAMVNQGRLKVLAV
                     TSSKRVRQFPNVPTMAEAGVSDFNITSWLGVYGPAGMPKAIVDQLSADIVAGVSQGEP
                     RTRLENVGFEVNPMRSEQWDRFNKEELARWSDIAKKAGISIEFGKG"
     gene            552..2102
                     /locus_tag="VM406_01910"
     CDS             552..2102
                     /locus_tag="VM406_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HVL74745.1"
                     /translation="MTGADILCETLLANGVDVCFANPGTSEMHFVAALDRQPRMRCVL
                     GLFEGVVTGAADGYARMADKPAATLLHLGPGLANGLANLHNARRASSPVVNIVGEHAS
                     YHLAHDAPLTSDIEGLARPMSGWVRTIGSADNISGDAAQAITAARGAPGQVATLILPA
                     DAAWGEAQQDGIAVAAPARLAPVTEAKLNEAAQALRSGKRTMILLTGKALRDGALDTA
                     SRIAQFSGAALAAERMAGRIPRGAGRVAIERIPYKLESAVSFLREFEQLILIGAREPV
                     AFFAYPGKPSRLVAPECKVMELAAPGDDLVQALENLAQALNLPTTLQPLRQERIDTGL
                     PSGALNADAVHAVVARMMPEQAIVCDESITSGQNLLRITRGAAPHDYLPLTGGAIGLG
                     LPMATGAAVACPGRKVISLQADGSGMYTLQALWTQAREQLDVVTIIFANRSYAILHGE
                     LKNVGAGEAGRNARRMLDLDDPAMNWVSLAEGMGVEAVRVATTEQLGAALQSALKRRG
                     PMLIEAVI"
     gene            2125..3402
                     /gene="gabT"
                     /locus_tag="VM406_01915"
     CDS             2125..3402
                     /gene="gabT"
                     /locus_tag="VM406_01915"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HVL74746.1"
                     /translation="MKTTNAALQERKIDATVPGVGVLTQVYAERASNAELWDVEGRRY
                     IDFTSGIGVLNTGHCHPKVVAAVEAQLKKFTHTCFQVFPYEPYVELAEKLNRLTPGTH
                     KKRTAFFTTGAEAAENAVKIARVATGRTGIIAFSGAFHGRTMMGMALTGKVMPYKAGF
                     GPFPADVYHAPFPVALHGVSVEDSLDALDRLFKADVDPQRVAAIFIEPVQGEGGFYVT
                     PPEFMRKLRAICDYHGILLVIDEVQTGFARTGKLFATEHYDVVPDMMLMAKGLAGGFP
                     LSAVTGRAEIMEAPKPGGLGGTYAGSPIAVAAALAVLDAIEQEGLVARANEIGAKLRN
                     TLEGVRGAVREIADVRGLGAMVAVEFNQPDTGRPDPDFAKTVQARALERGLMLLTCGI
                     HFNVIRFLIPLTIQHEVLDEGLEKLVDALTARA"
BASE COUNT          627 a         1178 c         1142 g          530 t
ORIGIN      
        1 ctgcatatcc cgtacaaggg cgccaacgaa gcgcagatcg atgtggccac cggcctggtc
       61 gacttcttct tcgccaacct gcccagcgcg ctggcaatgg tcaatcaagg ccgcctcaag
      121 gtcctggccg tgaccagctc caagcgcgtg cgccagttcc cgaacgtgcc gacgatggcc
      181 gaagccggcg tgtccgactt caacatcacc agctggctag gcgtgtacgg cccggccggc
      241 atgccgaagg ccatcgtgga ccagctcagc gccgacatcg tggccggcgt ttcccagggt
      301 gaaccgcgta cccgtctgga aaacgtcggc ttcgaagtga acccgatgcg ctccgaacaa
      361 tgggatcgct tcaacaagga agagctcgcg cgctggtcgg acatcgcgaa gaaggcaggc
      421 atcagcatcg agttcggcaa gggctgatct ccgccatcgt ccgcgagcct cggctcgcga
      481 gagccaacgt acagaccagc ctgcatatcc catgcaggct ggcacacatc tggaatctgg
      541 aacactgaac aatgaccggt gctgatatct tgtgcgagac cctcctggcc aatggagtgg
      601 acgtctgctt cgccaacccg ggaacctcgg aaatgcattt tgtcgcagcg ctggaccgcc
      661 agccgcgcat gcgctgcgtc ctcggcctgt tcgaaggtgt cgtcaccggc gcggccgacg
      721 gctacgcgcg catggccgac aagccggcgg ccaccctgct ccatctcggc ccggggctgg
      781 caaacgggct cgccaacctg cacaacgctc ggcgcgcgag ttcgccggtg gtcaatatcg
      841 tcggcgaaca tgcgagctat cacctggccc acgatgcgcc gctgacgagc gacatcgaag
      901 gactggccag gccgatgtcg ggctgggtgc gcacaatcgg gtcagcggac aacatttccg
      961 gcgatgccgc acaggccatc acggccgcgc gcggcgcacc cggccaggtt gcgaccctga
     1021 tcctgccggc cgacgcggca tggggcgagg cgcagcagga cggcatcgcc gttgccgcgc
     1081 cggcgcgcct ggcgccggtc acggaagcga agctcaatga agcagcgcag gccctgcgca
     1141 gcggcaagcg gaccatgatc ctgctgaccg gcaaggcctt gcgcgacggc gcgctggata
     1201 ccgccagccg catcgcccaa ttttccggcg cggcgctggc agccgagcgc atggccggcc
     1261 gcatcccccg cggcgccggc cgcgtggcga tcgaacgcat cccctacaag ctcgaatccg
     1321 cggtctcctt cctgcgcgaa ttcgaacaac tgatcctgat cggcgcgcgc gagccggtcg
     1381 ccttcttcgc ctatccgggc aagccgagcc gactggtcgc gccggaatgc aaggtgatgg
     1441 aactggcggc cccgggcgac gacctcgtcc aggcgctgga gaacctggca caggcgctga
     1501 acctgcctac cacgctgcag ccgctgcgcc aggaacgcat cgacaccgga ctgccttccg
     1561 gggcactcaa cgccgatgcc gtgcacgcgg tagtggcccg gatgatgccg gagcaggcga
     1621 tcgtctgcga cgagtcgatc acctccggcc agaacctgct gcgcatcacg cgcggcgcgg
     1681 ccccgcacga ctatctgccg ctgaccggcg gcgccatcgg cctcggcctg ccgatggcca
     1741 ccggcgccgc cgtcgcctgc cccggccgca aggtcatcag cctgcaggcc gacggcagcg
     1801 ggatgtacac gctgcaggcg ctgtggacac aggcgcgcga gcaactcgac gtggtcacga
     1861 tcatcttcgc caaccgctcg tacgcgatcc tccatggcga actgaagaac gtcggcgctg
     1921 gcgaagcggg gcgcaacgcg cggcgcatgc tggatctgga cgaccccgca atgaactggg
     1981 tgtcgctcgc cgaaggcatg ggcgtggaag cagtgcgcgt ggccaccact gaacaactgg
     2041 gcgcggccct gcaatcggcg ctgaagcggc gcggccccat gctgatcgaa gcagttatct
     2101 gaaccattca acgacggagc aaacatgaag acaacgaacg cggcgctgca ggaacgcaag
     2161 atcgacgcaa ccgttcccgg tgtgggcgta ctgacccagg tctacgcgga gcgtgcgagc
     2221 aacgccgagc tgtgggatgt cgaaggccgc cgctatatcg acttcacatc cggcatcggc
     2281 gtactcaaca ccgggcattg ccatccgaag gtcgtcgcgg cagtcgaagc gcagctgaag
     2341 aagtttacgc acacctgctt ccaggtcttt ccgtacgagc cgtatgtcga actggccgaa
     2401 aagctgaacc gcctcacgcc cggcacgcac aagaagcgca ccgccttctt caccaccggc
     2461 gccgaggcgg cggagaatgc ggtcaagatc gcacgcgtgg cgaccggacg caccggcatc
     2521 atcgcgttct cgggtgcgtt tcatggccgc acgatgatgg gcatggcgct gaccggcaag
     2581 gtgatgccgt acaaggccgg attcggcccc ttccccgccg atgtctatca cgcgccgttt
     2641 cccgtcgcgc tgcatggcgt gagcgtcgag gattcgctcg atgcgctcga ccgcctgttc
     2701 aaggctgacg tcgatccgca gcgcgtcgcc gcgatcttca tcgagccggt gcagggcgaa
     2761 ggcggcttct acgtgacgcc gccggagttc atgcgcaagc tgcgcgcgat ctgcgactac
     2821 cacggcatcc tgctggtcat cgacgaggtg cagaccggct ttgcgcgcac cggcaagctg
     2881 ttcgcgaccg agcactatga cgtcgtaccc gacatgatgc tgatggccaa gggcctggct
     2941 ggcggtttcc cgctctcggc cgtgaccggc cgcgcggaaa tcatggaagc gccgaagccg
     3001 ggcgggttgg gcggcaccta cgccggcagc ccgatcgcgg tggcggcggc gctcgccgtg
     3061 ctcgatgcga tcgaacagga gggactggtc gcgcgcgcca acgagatcgg cgcgaaactg
     3121 cgcaacacgc tggaaggcgt gcgcggcgcg gtgcgcgaga tcgccgacgt gcgcggcctg
     3181 ggtgcgatgg tggccgtcga gttcaaccag cctgatacag gccggccgga cccggacttc
     3241 gccaagacgg tgcaggccag agcgctcgag cggggactga tgctgttgac ctgcggcatc
     3301 cacttcaacg tgatccgctt cctgatcccg ctcacgatcc agcacgaagt actggacgag
     3361 gggctggaga aactggtgga tgcgttgact gcacgcgctt gaaggagatc gtgcgctgcc
     3421 cgccttcccc gcgcgggcag cgcgatctgt cgaatcggcg ccacgttttc tcgtgac
//
LOCUS       DATLGF010000061         2938 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_144043, whole genome shotgun sequence.
ACCESSION   DATLGF010000061 DATLGF010000000
VERSION     DATLGF010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144043"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VM406_01920"
     CDS             <1..260
                     /locus_tag="VM406_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HVL74747.1"
                     /translation="VMVAAGDAPMKLSAQNLLAGTVARLVRGAVSAEVVIQLPGGATI
                     CAIVSNDSVDAMQLGEGQPATAIINASHVILGVPASYQETP"
     gene            257..1006
                     /gene="modA"
                     /locus_tag="VM406_01925"
     CDS             257..1006
                     /gene="modA"
                     /locus_tag="VM406_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HVL74748.1"
                     /translation="MKRLLAALLFCVSGAAVADVLTVAVAANVQYAFSAIAEAFQKES
                     NHRIRPIYNSSGKLTAQITHGAPFDVFLSADMEYPQALHRSGHAPQAPRVYASGALVL
                     WTMHPALDLQDWRNALVSSQVRRIAIANPKLAPYGREAMKAFAHFGLADAITPKLVYG
                     ESVSQVNQYTHSRAVDAGITAKSVVMAPEMQGQGRWVELPQDSYVPIEQGIVVLAYGR
                     RNNPVAAQQFHDFVLSPAGRAILSRYGYLLP"
     gene            1003..1413
                     /locus_tag="VM406_01930"
     CDS             1003..1413
                     /locus_tag="VM406_01930"
                     /inference="COORDINATES: protein motif:HMM:NF015424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HVL74749.1"
                     /translation="MNRLPAIVRAVEIDGSIALVDAQVGAHSYASLMVGASSEAAEWK
                     VGEPVELVFAETEVALATQLGGTLSMRNRFPATVRTIESGRILTRVAMEFDGHLLHAL
                     VTTRAARELDLQVGDAVTGLVKANEMTVTNPSAQ"
     gene            1410..2087
                     /gene="modB"
                     /locus_tag="VM406_01935"
     CDS             1410..2087
                     /gene="modB"
                     /locus_tag="VM406_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HVL74750.1"
                     /translation="MNHDWEPLLLTFRLAAITTAILFVIGVPLACWIAYTRVRIKPVI
                     ETLVAMPLVLPPSVLGFYLLLAFSPQNAFGRRLDEWFGIQLVFSFPGLVIGSVIFSLP
                     FMVHPIQSGLQNLPRSLLEASRTLGKSDLQTLFRILLPNIKPALLSGGVLSFAHTVGE
                     FGVILMIGGNIPGVTKVASIAIYDEVESLNYTAANFYAMVLFAVSFAILLMVYLMNKK
                     LVRAGGP"
     gene            2084..>2938
                     /locus_tag="VM406_01940"
     CDS             2084..>2938
                     /locus_tag="VM406_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVL74751.1"
                     /translation="MIVIDIARQLHSAAGPMRLAFAARIERHELATLFGPSGAGKTTI
                     LRLLAGLASPDEGRIEVDGVAWFDSARRIDLPARQRSIGFVFQDYALFPNLTVRENIA
                     YALETKDEGWVDELVALADLKSLQQRMPATLSGGQKQRVALVRALARKPALLLLDEPL
                     SALDHAMRVQLQDDLLRMHQRFGLTTMLVSHDVGEVFRLSRRVLRIEGGRLVEDGSPE
                     QVFLHRRMAGRFNLRAQVLSVRRQDVVHVVALLIGQEITEVIASEDEIAGLRPGDFVS
                     VSSKAFSPI"
BASE COUNT          487 a          974 c          960 g          517 t
ORIGIN      
        1 gggtcatggt ggccgccggc gatgcgccga tgaagctttc cgcgcagaac ctgctggccg
       61 gcaccgtggc gcgcctggtc cgcggcgcgg tcagtgccga ggtagtgatc cagcttcccg
      121 gcggcgcgac gatctgcgca atcgtcagca atgacagcgt ggacgcgatg cagctcggcg
      181 aaggacagcc ggccaccgcc atcatcaatg cctcgcatgt gatcctcggc gtgcccgctt
      241 cgtatcagga gacgccatga aacgcttgct tgcagcgctg ctgttctgcg tcagcggcgc
      301 agccgttgcc gacgtgctga ccgtcgccgt tgccgcaaac gtccagtacg cgttctccgc
      361 gatcgcggaa gcctttcaga aagagtcgaa ccaccggatc cggccgatct acaactcgtc
      421 cggcaagctg accgcgcaga tcacccatgg cgcgccgttc gacgtcttcc tgtcggcgga
      481 catggaatac ccgcaggcat tgcaccgctc cggccacgca ccgcaagcac cgcgcgtcta
      541 cgccagcggc gcgctggtgc tgtggaccat gcacccggcc ctggacctgc aggactggcg
      601 caacgcgctg gtctcgagcc aggtgcgtcg gatcgcgatc gccaatccca agctggctcc
      661 ttacggccgc gaagcaatga aggcattcgc gcacttcggg ttggccgacg ccatcacgcc
      721 caagctcgtg tacggcgaga gcgtctcgca ggtgaaccag tacacgcact cgcgcgcggt
      781 cgatgccggc atcaccgcga agtcggtcgt gatggcgccg gaaatgcagg gacaggggcg
      841 ctgggtcgaa ctgccgcaag actcgtatgt acctatcgaa cagggcatcg tcgtgctggc
      901 ctacggccgc aggaacaatc cggtcgccgc gcagcagttt cacgacttcg tgctgtcccc
      961 tgccgggcgc gcgatccttt cccgctacgg ctacctgctc ccatgaaccg cctgcccgcc
     1021 atcgtgcgtg ccgtggaaat cgacggcagc atcgcgctgg tcgatgcgca ggtcggcgcc
     1081 cattcatatg cgtcgctgat ggtcggcgca agcagcgagg cggcggaatg gaaagtcggc
     1141 gaacctgtgg aactggtgtt cgccgaaacc gaggtggcgc tggccacgca actgggcggc
     1201 accctcagca tgcgcaaccg ctttcctgcc acggtgcgca cgatcgagtc cgggcgcatc
     1261 ctgacgcgcg tcgcgatgga gttcgatggg catctgctgc acgcgctcgt gaccacccgc
     1321 gccgcgcgcg aactggacct gcaggtcggc gatgcggtga ccggcctcgt caaggccaat
     1381 gaaatgacgg tgaccaaccc gtccgcgcaa tgaatcacga ctgggaaccg ctgctgctca
     1441 cattccggtt ggccgcgatc acgacggcga tcctgttcgt gatcggcgtg ccgctggcat
     1501 gctggattgc gtacacgcgc gtgcgcatca agccggtgat cgagacgctg gtggccatgc
     1561 cgctggtgct gccgccctcg gtgctgggct tctacctgct gctcgcgttc agcccgcaga
     1621 acgcgttcgg acgcaggctg gacgaatggt tcggcataca gctggtgttc agctttcccg
     1681 ggctcgtgat cggctcggtg atcttcagcc tgccgttcat ggtccatccg atccagtccg
     1741 gcctgcagaa cctgcccagg tcgctgctgg aagcctcgcg cacgctcggc aagtccgatc
     1801 tgcaaacgct gtttcgcatc ctgctgccaa acatcaagcc cgccctgctc tccggcggcg
     1861 tgctcagctt cgcccatacg gtaggcgagt tcggcgtgat cctgatgata ggcgggaaca
     1921 ttcccggcgt gaccaaggtc gcgtcgatcg cgatctacga cgaagtcgaa agcctgaact
     1981 acacggcggc caacttctac gcgatggtgc tgttcgcggt ttcgttcgcg atcctgctga
     2041 tggtctatct gatgaacaag aagctggtgc gggcgggcgg cccatgatcg tcatcgacat
     2101 tgcacggcaa ctgcacagcg ccgccgggcc gatgcgcctc gcgttcgccg cgcgcatcga
     2161 gcgccacgag ctcgccacac tgttcggtcc gtccggcgcc ggcaagacca cgatcctgcg
     2221 cctgctggca ggactggcca gcccggacga agggcgcatc gaggtcgacg gcgtcgcctg
     2281 gttcgactcc gcgcgccgca tcgacctgcc cgcccgtcag cgcagcatcg gcttcgtgtt
     2341 ccaggactac gcgctgtttc cgaacctgac ggtgcgcgag aacatcgcct atgcgctgga
     2401 aacgaaggac gagggctggg tcgacgaact ggtggcgctt gcggatctaa agtccttgca
     2461 gcagcgcatg ccggccacgc tgtcaggcgg ccagaagcag cgcgtcgcgc tggtgcgcgc
     2521 actggcgcgc aagccggccc tgctgctgct ggacgaaccg ctgtcggcgc tcgatcatgc
     2581 gatgcgcgtg cagctgcagg acgatctgtt gcgcatgcat cagcgtttcg gtctcacgac
     2641 gatgctggtg agccacgacg tcggcgaagt gttccggctg tcccggcgcg tgctgcgcat
     2701 cgaaggcggc cgcctcgtcg aggacggcag ccccgagcag gtcttcctgc acaggcgcat
     2761 ggccggcagg ttcaacctgc gcgcgcaggt gctgtcagtg cgccgccagg acgtggtcca
     2821 tgtcgttgcg ctgctgatcg gccaggagat caccgaggtg atcgcgtcgg aagacgagat
     2881 tgcgggactc agaccgggcg acttcgtctc ggtgtcgagc aaggcgttca gcccgata
//
LOCUS       DATLGF010000062         3318 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_207304, whole genome shotgun sequence.
ACCESSION   DATLGF010000062 DATLGF010000000
VERSION     DATLGF010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207304"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..675
                     /locus_tag="VM406_01945"
     CDS             49..675
                     /locus_tag="VM406_01945"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVL74752.1"
                     /translation="MKNLSHPALAAGGEAGWSERLLSAQRRKLFEAFTQFRQGGADDT
                     VLEVRHADAAWMRNWTESPPKSLVAACAIDPALPALRLPYPDNAFDWVFCPEVIEHAG
                     APERQQRLVAECYRVARKGVFLTTPNRRHPLEFNTGLPFAHWLPDTLYRRCLERFPRR
                     QGPLALLDSARLYRLAEALPGAPAHDVGHKRVFGIKAHFFLMVSKPGR"
     gene            903..1388
                     /locus_tag="VM406_01950"
     CDS             903..1388
                     /locus_tag="VM406_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL74753.1"
                     /translation="MPTFSIRFAAPADVPAILAMIRELAEFEKLTHLFVADETALAEA
                     LFAERPHCECLVGEEDGRPVAFALFFHNFSTFLARRGLYLEDLYVKPEHRGRGHAEAM
                     LRELARIAVERGCGRFEWSVLDWNENAIRFYRKMGAEVMPDWRICRVTGDGLSALARQ
                     G"
     gene            1592..1780
                     /locus_tag="VM406_01955"
     CDS             1592..1780
                     /locus_tag="VM406_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74754.1"
                     /translation="MKKILTVLATLALLSGCANGTFGGMTGGPGYGHGASGAHGQDVR
                     TNPMNHPLNPAPYYGGGI"
     gene            complement(2028..>3318)
                     /gene="ettA"
                     /locus_tag="VM406_01960"
     CDS             complement(2028..>3318)
                     /gene="ettA"
                     /locus_tag="VM406_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HVL74755.1"
                     /translation="LEAVIAAAGAEGDAEHLLEIAADALRLPPWDAKIEHLSGGEKRR
                     VALCKLLLSRPDMLLLDEPTNHLDAESVDWLEQFLTRFPGTVVAVTHDRYFLDNAAEW
                     ILELDRGHGIPWKGNYSSWLDQKETRLKQEESSESARQKALKKELEWVRQNPKGRQAK
                     SKARLARFNELSDHEYQKRNETQEIFIPVAERLGNEVIEFKNVSKAYGDRLLIDNLSF
                     RIPAGAIVGIIGPNGAGKSTLFRMIAGREKPDSGEIVVGPTVKLSLVDQSREELSNKK
                     TVWEDISNGADILTVGRFEMPSRAYLGRFNFKGADQQKIVGNLSGGERGRLHLAKTLL
                     MGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLDRIATHILAFEGDS
                     QVVFFDGNYQEYEADKKKRLGEEAAKPKRIRYKPITR"
BASE COUNT          533 a         1086 c         1064 g          635 t
ORIGIN      
        1 cgataatgtt cgcgtggtca aagcggccgc gccatttgcg gaggagtgat gaaaaacctg
       61 agccatcccg cgcttgcagc cggcggcgaa gccggctgga gcgagcgcct gctgagcgcg
      121 cagcgccgca agctgttcga ggcgttcacc cagttccgcc agggcggcgc cgacgacacc
      181 gtgctggaag tgcgccacgc cgacgccgcc tggatgcgca actggaccga atcgccgccg
      241 aagtcgctcg tggccgcctg cgcgatcgat ccggcgctgc cggccttgcg gctgccatac
      301 ccggacaatg ccttcgactg ggtattctgt ccggaggtca tcgagcatgc cggcgcgccc
      361 gagcgccagc aaaggctggt cgccgagtgc tacagggtcg cgcgcaaggg cgtattcctg
      421 accacgccga accgccgcca tccgctcgaa ttcaataccg gactgccgtt tgcgcactgg
      481 ctgccggaca ccttgtaccg gcgctgcctc gaacgcttcc cgcggcgcca gggaccgctg
      541 gcgctgctcg actcggcgcg gctgtaccgc ctggccgaag ccctgcccgg cgcccccgcg
      601 catgatgtcg gccacaagcg ggtgttcggc atcaaggcgc atttctttct gatggtcagc
      661 aagccgggtc gctgacttta cggacaggcg cgagctgtgc atcaacgcca ctggaccccg
      721 gctttcgccg gggtgacgcg ctgttgggcg cgcagtcgag ctcagcatcg gtcatcccgg
      781 cgaaagccgg gatccagcgg cgtttctcca tttgccacgg gcacgcattg acgcctcggc
      841 gcctcgtgca tcctttgccc aaggcctaca atggtgcgtt tcgaacaacg acgcacattg
      901 ccatgccgac cttttccatc cgatttgcag cccccgccga cgtacccgcg atcctcgcca
      961 tgatccgtga gctcgcggag ttcgagaaac tgacgcacct gttcgttgcc gatgaaaccg
     1021 cgctcgccga agccctgttc gccgagcgcc cccattgcga atgcctggtc ggcgaggaag
     1081 acggccggcc ggtcgcgttc gcgctgttct tccacaactt ctcgaccttc cttgcccggc
     1141 gcggcctgta tctcgaggat ctgtacgtca agcccgaaca ccgcgggcgc ggccatgccg
     1201 aggcgatgct gcgcgaactg gcgcgcatcg ccgtcgagcg cggctgcggc cgctttgaat
     1261 ggtcggtgct ggactggaac gagaatgcga tccgctttta ccgcaagatg ggcgcggagg
     1321 tgatgccgga ctggcgcatc tgccgcgtga ccggcgacgg cctgagcgca ctggcgcggc
     1381 aagggtgacg gccccgggga cgcgcaggac accgccgtgt tccggcacgc gcctgcgtga
     1441 cgcttcagaa gccgatggaa gggttttcgg gacctgtcct gatattgctc aataaatccg
     1501 cctgtggtgt ttgtggtccc gcaggcaggc gcgcgcgact cctatactgg ccgatgaagg
     1561 gagaagggga agtcttcatt cggagccaac aatgaagaag atactcactg ttctcgcaac
     1621 ccttgcgctg ctttcgggct gtgccaacgg cacgttcggc ggcatgaccg gcggtcctgg
     1681 ctacgggcat ggcgcctccg gcgcccacgg ccaggacgtc cgcaccaatc cgatgaacca
     1741 tccgctcaat cccgctccgt actacggcgg cggaatctga tcggcaggct tcggtttaat
     1801 tgcggcacaa gaaatccggc gagcgacgac tgccgtcagc ttccgtatcc cggcattccg
     1861 tcgtcccggc gaaagccggg attcaacgac gtgccggctc tgcatccgcg ccactggacc
     1921 tcggctttcg ccggggtgac gtcccggttt gaggtcaagt ggcgaggcct cgagtgaacg
     1981 gcatgacggc ttgcgccggc ttttggctcg cgtcggattt ccttccgtca gcgggtgatc
     2041 ggcttgtaac ggatgcgctt cggcttggcc gcttcctcgc ccagccgctt tttcttgtcg
     2101 gcctcgtact cttggtagtt cccgtcgaag aacaccacct gtgaatcgcc ctcgaacgcg
     2161 aggatgtggg tggcgatgcg gtcgaggaac cagcggtcgt gcgagatcac catcacgctg
     2221 ccggcgaatt ccagcagcgc atcttccagc gcgcgcaggg tttccacatc caggtcgttc
     2281 gacggttcat cgagcagcag cacgttgccg cccatgagca gggtcttggc caggtgcagg
     2341 cgtccgcgtt cgccgcccga aagattgccg acgatcttct gctggtcggc gcccttgaag
     2401 ttgaagcggc ccagataggc gcgcgacggc atctcgaaac ggccgacggt gaggatgtcg
     2461 gcgccattgg agatgtcttc ccagacggtc ttcttgttcg acaactcctc gcgcgactgg
     2521 tccaccagcg acaacttgac ggtcgggccg acgacgatct cgccggaatc cggcttttcc
     2581 ctgccggcga tcatgcgaaa cagcgtggac ttgccggcgc cgttgggacc gatgatgccg
     2641 acgatcgcac cggccgggat ccggaacgac aggttgtcga tcagcaggcg gtcgccatag
     2701 gccttcgaga cattcttgaa ctcgattact tcattaccga ggcgctcggc caccgggatg
     2761 aagatttcct gcgtctcgtt gcgcttctgg tattcgtggt ctgagagttc gttaaagcgg
     2821 gccaaacgtg ccttgctctt ggcctggcgg cctttcggat tctggcgcac ccattccagt
     2881 tctttcttca acgccttttg gcgcgccgat tcgctcgact cttcctgctt caggcgcgtc
     2941 tctttctgat cgagccagga gctgtaattg cccttccagg ggatgccgtg gccgcggtcg
     3001 agttcgagaa tccactcggc ggcgttgtcg aggaagtagc ggtcgtgggt cacagccaca
     3061 acggtgccgg ggaagcgcgt gaggaactgc tcgagccagt ccaccgattc tgcatcgagg
     3121 tggttggtcg gctcgtccag cagcagcatg tcggggcgcg acagcagcag cttgcacagc
     3181 gccacgcggc gcttctcacc gcccgacagg tgctcgatct tggcgtccca cggcggcagg
     3241 cgcagcgcgt cggcggcgat ctccagcagg tgctcggcgt cgccctcggc gccggcggcg
     3301 gcgatcacgg cttccagc
//
LOCUS       DATLGF010000063         8654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_112533, whole genome shotgun sequence.
ACCESSION   DATLGF010000063 DATLGF010000000
VERSION     DATLGF010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 8654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8654
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112533"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2232
                     /locus_tag="VM406_01965"
     CDS             <1..2232
                     /locus_tag="VM406_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL74756.1"
                     /translation="LLRLLHTLKGSARMAGAMNLGQHLHDMETRAENIMRLGLPSGIA
                     IDDLLARHDQGLQMFEELQNPGARKAAAAEAAAAFQAPQPESDAPTLQPLVPALARTS
                     LATAPERQVATPAGSTPLVRVRADILDRLVNQAGEVSISRSRLETEVGTLRQSLGELT
                     ENVGRLREQLREIEMQAESQIASRMQQSGDREFDPLEFDRFTRLQELTRMMAESVNDV
                     GSVHHTLSRTLDSATGDLGTQARLTRELQQDLMRVRMVQFASISERLFRVTRQAAKEV
                     DKRVNLDIRGGGVEVDRGVLEKMAGPFEHLLRNAIVHGIESREARRAAGKNEIGELLV
                     EVRQEGNEVVLQFSDDGQGLNLARIRAKGERLGLIGPDTPATDGEVADLIFHPGFSTA
                     EEITELAGRGVGMDVVRSEAATLGGRVESHSEPGRGTRFTIRLPLTLAVTQVVILSTG
                     GKTYAVPSVLVEQVQQLKANALAAAYNEGAVMWQGQRVAIHYLSALLGDTKAAPVAQQ
                     YTPVMILKSGSERVALHVDEVVGNREVVVKNIGPQLARMIGIAGATVLGSGDIVLILN
                     PVPLAQRAALDDMRAPRLIDSDAPQHMGAVAELSHAAPRVADPEPVQGLRTQNIVMVV
                     DDSLTVRRVTQRLLSREGYQVVLAKDGVDALEQLQSITPDVMLVDIEMPRMDGFDLTR
                     NVRDDQRTRHIPIIMITSRTAGKHRNYALELGVNEYLGKPYQEERLLELISGYVSNRV
                     SAS"
     gene            2624..3298
                     /locus_tag="VM406_01970"
     CDS             2624..3298
                     /locus_tag="VM406_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HVL74757.1"
                     /translation="MASMVKPSKSRAFSSSPGAPQDAPDFSGAANASPGSDFQLADHF
                     LIAMPSMLDPVFGGTLVYLCEHNAEGALGVIVNKPTDMTVATLLERVDLKLEIAPSES
                     ALGDQPVLFGGPVQVERGFVLHTQHGPYSSTLKVSEEVALTTSRDILEAVATGRGPRR
                     LLITLGCAGWSAGQLEQEIVNNGWLTVRADPAIMFELPPEQRFVAAMKLLGIDPAMLA
                     GVAGHA"
     gene            3328..3717
                     /gene="ruvX"
                     /locus_tag="VM406_01975"
     CDS             3328..3717
                     /gene="ruvX"
                     /locus_tag="VM406_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HVL74758.1"
                     /translation="METVLAFDFGLKRIGVAVGNSLTRHAQPLTVIQAATNDAKFEAI
                     GKLIEAWQPKRCIVGLPMHPDGAEHEMSVRCRRFANQLNGRFGVATTLVDERYSSAVL
                     QQRRGETIDAEAAAIILQQYFNDHAES"
     gene            3704..4219
                     /gene="pyrR"
                     /locus_tag="VM406_01980"
     CDS             3704..4219
                     /gene="pyrR"
                     /locus_tag="VM406_01980"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HVL74759.1"
                     /translation="MPNPEFDAEALYRVLADRVKAGLAETGDPAAIAIVGIYSGGAWL
                     AERLAAELQTTQPVGFIDVSFYRDDYAEKGLHAEVQPTQIPFSVEGATILLVDDVLYT
                     GRTTRAAINELFDYGRPARIMLAALVDRGGRELPVAADFVAQTVSLAKDQKLDLKRAE
                     DGRLSLTVKHA"
     gene            4212..5171
                     /locus_tag="VM406_01985"
     CDS             4212..5171
                     /locus_tag="VM406_01985"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HVL74760.1"
                     /translation="MHNPQLNKNGELQHLLTIEGLPRSVITHILDTASSFVSIGDREV
                     KKVPLMRGKSVFNLFFENSTRTRTTFEIASKRLSADVINLNIAASSTSKGESLLDTID
                     NLSAMHADMFVVRHAQSGAPYLIAKHLIDTKQSHVHVVNAGDGRHAHPTQGLLDMYTI
                     RHYKKDFTNLRVAVVGDILHSRVARSDIHALTTLGVPEVRAIGPRTLLPGGLEQMGVR
                     VFTDINQGLKDVDVIIMLRLQNERMSGALLPSAQEFFKSYGLTPERLALAKPDAIVMH
                     PGPMNRGVEIDSAVADGAQAVILPQVTFGIAVRMAVMSILAGN"
     gene            5175..6458
                     /locus_tag="VM406_01990"
     CDS             5175..6458
                     /locus_tag="VM406_01990"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HVL74761.1"
                     /translation="MKIEIKNGRVIDPANGIDAQRNVYIADGRIAAVGEAPAGFTADQ
                     TIDAAGMIVVPGLVDLSARLREPGYEYKATLESEMQAAMQGGVTSLLCPPDTDPVLDE
                     PGLVEMLKHRARNLNQANVYPLGALTMGLRGEALTEMAELTEAGCIGFAQAEQPIGDT
                     TVMLRALQYAKTFGYTVWLRPQDPHLGRNGNAHSGAVASRLGLAGVPVMAETIALYTI
                     FELVRATGARVHLCRISSAAGLELMRAAKKEGLPVSCDVGVHHIHLTDLDIGFFDSNA
                     RLTPPLREQRDRDAIRAALADGTIDAICSDHTPVDDDEKLLPFGEATPGATGLELLLP
                     LTLKWAGECLGDKGLSTAIARITSDAARVAGLAAGALTRGAAADLCVFDPYARWKVEA
                     KALASQGKHTPFIGYELEGQVRATVVAGRVAYQRG"
     gene            6525..7289
                     /locus_tag="VM406_01995"
     CDS             6525..7289
                     /locus_tag="VM406_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HVL74762.1"
                     /translation="MRLLRLLWLILHLFRGLATCALIFPFVDVRGREHHIRRWSHALL
                     RICRVELRVIRNDEAEDLPRALIVANHHSWLDIFVINAMHPCRFVAKSEIRKWPLIGW
                     LCEQVGTIFIERGRLRDVRRIYQGLVESLHAGEHVAFFPEGTVSEQGSLHPFHANLFE
                     AAIEAAVPVQPFALRYLDRAGNYAESIRYVVPITFPQSLWMILGGPPIVAELRLLAPI
                     PTPHGAHRRDLANAARAAIAPALGIPTAEVQTEPGA"
     gene            complement(7268..8089)
                     /locus_tag="VM406_02000"
     CDS             complement(7268..8089)
                     /locus_tag="VM406_02000"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HVL74763.1"
                     /translation="MSTYVIGDIQGCGERLEVLLRKIEEEDAGASFVFVGDLVNRGPR
                     SLETLRRVRALGERARTVLGNHDLHLLAVANDIRAPHRSDTLDPILQAADSRELLDWL
                     RKQPLALEVNGHLVVHAGVLPQWTAAQTLALAQEVESVLRGPDWVEFLKRMYGNTPAR
                     WSDDLEGADRLRCIVNALTRLRFCSADGTMELTSKGVETEMPGYMPWFDVPGRKTEAV
                     TVVFGHWSTLGLVLRPNLISLDTGCLWGGKLTAVRLSDRHVVQVNCPQYQAPGSV"
     gene            8237..>8654
                     /locus_tag="VM406_02005"
     CDS             8237..>8654
                     /locus_tag="VM406_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HVL74764.1"
                     /translation="MLIPIILSGGAGTRLWPVSREAHPKPFMRIGGGKSLIAQTCERA
                     QTTSPGGMSLVVTNREYYLRTREELAKQDFMPHYLLEPVGRNTAPAVAAAALWALRQD
                     ADACLLVMPADHLITDTAAFQQAAKYAEMLARDGFLV"
BASE COUNT         1553 a         2914 c         2759 g         1428 t
ORIGIN      
        1 ctgctgcgcc tgctgcatac cctcaagggc agcgcgcgca tggccggcgc gatgaatctc
       61 ggccagcatc tgcacgacat ggaaacgcgt gccgagaaca tcatgcgtct cggcctgccg
      121 tcgggtatcg ccatcgacga cctgttggct cgccacgacc agggcctgca gatgttcgag
      181 gaactgcaga atcccggtgc gcgcaaggcc gccgcagccg aagcggccgc cgccttccag
      241 gcgccgcagc cggaatcgga tgcgccgacg ctgcagccgc tcgttccggc gctcgccagg
      301 acatcgctcg cgacagcgcc cgaacggcag gtggcaacgc cggccggttc gaccccgctg
      361 gttcgcgtgc gcgccgacat cctcgaccgc ctggtcaacc aggccggtga agtctcgatc
      421 tcgcgctcgc gcctggaaac cgaggtcggg accctgcgcc agtccctggg cgaactgacc
      481 gagaacgtcg gccgcctgcg cgagcagttg cgcgagattg agatgcaggc cgagtcacag
      541 attgcttcgc gcatgcagca atcgggcgac cgcgagttcg acccgctcga gttcgaccgc
      601 ttcacccgcc tgcaggagct gacccggatg atggccgaga gcgtcaacga cgtcggctcg
      661 gtgcaccaca cgctgtcccg tacgctcgac agcgcgaccg gcgacctggg cacccaggcc
      721 cggctgacgc gcgaattgca gcaggatctg atgcgcgttc ggatggtgca gttcgccagc
      781 atttcggagc gcctgttccg cgtcacccgc caggccgcca aggaagtgga caagcgggtc
      841 aacctggaca tccgcggcgg cggcgtggaa gtcgaccgcg gcgtgctgga aaagatggcc
      901 ggcccgttcg agcacctgct gcgcaatgca atcgtgcacg gcatcgagtc acgcgaagcg
      961 cgacgcgcgg ccggcaagaa cgagatcggc gaactgctgg ttgaagtgcg ccaggagggc
     1021 aacgaggtcg tcctgcaatt ctccgacgac ggtcagggac tgaacctggc ccgcatccgc
     1081 gccaagggcg agcgcctcgg gctgatcggc ccggacacgc cggctaccga cggcgaagtt
     1141 gccgacctga tcttccatcc cggattctcg acggcggaag aaatcaccga gctcgctggc
     1201 cgcggcgtcg gcatggacgt ggtccgctcc gaagccgcca cactgggcgg ccgggtcgaa
     1261 tcgcattccg aaccggggcg cggcacgcgc ttcacgatcc gcctgccgct gacgcttgca
     1321 gtcacccagg tcgtgatcct gagcaccggc ggcaagacct atgcggtgcc ctccgtgctc
     1381 gtggagcagg tccagcagct gaaggcaaat gcactggcgg ccgcgtacaa cgagggtgcg
     1441 gtgatgtggc agggacagcg cgtggccatc cactacctgt ctgcgctgct cggcgatacc
     1501 aaggcagccc cggtggccca gcagtacacg cctgtgatga ttctcaagag cggcagcgaa
     1561 cgcgtggccc tgcatgtgga cgaagtcgtc ggcaaccgcg aagtggtggt caagaacatc
     1621 ggcccgcagc ttgcgcggat gatcggcatt gcaggcgcaa ccgtgctcgg ttccggcgac
     1681 atcgtgctga tcctgaaccc ggtgccgctg gcccagcgcg ccgccctcga cgacatgcgc
     1741 gccccgcggc tgatcgactc ggacgcaccg cagcacatgg gcgcggtggc cgaactgtcc
     1801 cacgctgcgc cgcgcgtggc cgatcccgag ccggtgcagg gcctgcgcac gcagaacatc
     1861 gtgatggtgg tcgacgattc cctcaccgtg cgccgcgtca cgcaacgcct gctctcgcgt
     1921 gaaggctatc aggtggtgct cgccaaggac ggcgtcgacg cgctcgagca attgcagtcg
     1981 atcacgccgg acgtgatgct ggtggatatc gaaatgccgc gcatggacgg cttcgacttg
     2041 acccgcaacg tgcgcgacga tcagcgtacc cgtcatattc cgatcatcat gatcacctcg
     2101 cgcacggccg gcaagcatcg caattacgcg ctcgagctgg gcgtcaacga gtacctcggc
     2161 aagccgtatc aggaagagcg tttgctggag ttgatttcgg gatatgtgag caaccgggtg
     2221 agtgcgtcct gaggcaatgt cgtttctgcg cgggcgcact cctgttcgga atacttgtgc
     2281 ggattcaggc aacgccttgt tctggccggc tccgccacgt gcaagagcga tgcgcgactt
     2341 gatcgtgatg ttgggaaggg gtggcgcgta aggcgtgcca tcgcccgatt cctgtcgtcc
     2401 cgaacgtagt gagggatctc atcgccctgg caagcgaggg cgcagagcaa gcggatgagg
     2461 tcaacgaact aattccggac agcaatgcgc cttcgcggcg gccgcggagg gggacgccac
     2521 tgattgacat gtccacatca acgcaatcga ccccaaactc gcatcagctt cgacgatcgc
     2581 ccgcccgccc ggccaagccg gccggcacac caaagctgat aaaatggcca gcatggtcaa
     2641 acccagcaag tcccgcgcat tttcctcatc tcctggcgcc ccgcaggacg cgcccgactt
     2701 ttccggggcc gccaacgcca gccccggatc cgactttcag ctcgccgacc atttcctgat
     2761 cgccatgccg tcgatgctcg acccggtgtt cggcggcacg ctggtgtatc tgtgcgaaca
     2821 caatgccgaa ggtgcgctgg gcgtcatcgt caacaagccg accgacatga cggttgcaac
     2881 gctgctcgaa cgggtggacc tcaagctgga gatcgcgccg tcggaaagcg cgctgggtga
     2941 ccagccggtg ctgttcggcg gaccggtcca ggtcgagcgc ggcttcgtgc tgcatacaca
     3001 gcatgggccg tactcgtcga cgctgaaggt cagcgaggaa gtggccctca ccacttcgcg
     3061 cgacatcctc gaggcggtag cgaccggccg cggcccgcgc cgcctgctga tcacgctggg
     3121 ttgcgcaggc tggagcgccg gccagctcga gcaggaaatc gtcaacaacg gctggctcac
     3181 cgtgcgtgcc gacccggcaa tcatgttcga gctccccccg gagcagcgct ttgttgccgc
     3241 gatgaagctg ctcggcatcg atccagcgat gctggccggt gtggcgggcc atgcctgacc
     3301 cggaccgcct cgtatccaac ccctgtcgtg gaaaccgtac tggcattcga tttcggcctc
     3361 aagcgcatcg gcgtggcggt cggcaattcg ctgacgcgtc atgcccagcc gttgacggtg
     3421 atccaggccg cgaccaacga tgcaaagttc gaagcgatcg gcaagctgat cgaggcctgg
     3481 cagccgaagc gttgcatcgt cggcctgccg atgcatccgg acggcgccga gcacgagatg
     3541 agcgtgcgat gccgccgctt cgcaaatcaa ttgaacggcc gtttcggcgt ggcgaccacg
     3601 ctggtggacg agcgttactc gtcggccgtg ctgcagcagc ggcgcggcga aaccatcgat
     3661 gccgaagcgg ccgcaatcat tctccaacaa tacttcaatg accatgccga atcctgaatt
     3721 cgacgccgag gccctgtacc gggtgctcgc cgaccgcgtc aaggccgggc tggccgagac
     3781 cggcgatcct gccgccattg ccatcgtcgg catctactcc ggcggcgcct ggctggccga
     3841 gcggctcgcc gccgaactcc agaccacgca gccggtcggc ttcatcgacg tctcgttcta
     3901 tcgtgacgac tatgccgaga agggcctgca tgccgaggtg cagccgaccc agatcccgtt
     3961 cagcgtggag ggcgcgacga tcctgctcgt ggacgacgtg ctctataccg gccgcaccac
     4021 gcgtgcagcg atcaacgaac tgttcgacta cgggcgccct gcgcgcatca tgctcgccgc
     4081 gctggtcgac cggggcggac gggagctgcc tgtggcggcc gatttcgtcg cgcagacggt
     4141 aagtctcgcg aaggaccaga aactcgatct gaagcgtgcc gaagacggcc gcctctccct
     4201 gacggtgaaa catgcataac ccgcaactga acaagaatgg cgagctgcag cacctgctca
     4261 ccatcgaagg cctgccccgg tctgtcatca cgcacatcct cgataccgca tcctcgttcg
     4321 tcagcatcgg cgaccgcgaa gtgaagaagg tgccgctcat gcgcggcaag agcgtcttca
     4381 acctgttctt cgagaattcg acgcgtaccc gcaccacatt cgagatcgca tccaagcgtc
     4441 tgtcggccga cgtgatcaac ctgaacatcg cggcctccag cacttccaag ggcgaatcgc
     4501 tgctcgacac catcgacaac ctctcggcca tgcatgccga catgttcgtg gttcggcatg
     4561 cgcagtcggg cgcgccctac ctgattgcca agcacctgat cgacacgaag cagagccacg
     4621 tgcatgtcgt caacgccggc gacggccgcc atgcgcaccc aacccagggc ctgctggaca
     4681 tgtacacgat ccgccactac aagaaggatt tcacgaacct gcgcgtggcg gtcgtcggcg
     4741 acatcctgca cagccgggtt gcgcgctccg acatccatgc gctcaccacc ctgggcgtgc
     4801 cggaagtgcg cgcgatcggc ccgcgcacac tgctgccggg cgggctggag cagatgggcg
     4861 tgcgcgtgtt caccgacatc aaccagggcc tgaaggacgt ggacgtcatc atcatgctcc
     4921 gcctgcagaa cgagcgcatg tccggcgcct tgctgccctc ggcccaggaa ttcttcaaga
     4981 gctacggcct gacgccggag cggctggcgc tggccaagcc tgacgcgatc gtgatgcacc
     5041 ccggaccaat gaaccgcggc gtggagatcg actccgccgt ggccgacggc gcccaggcgg
     5101 tgattcttcc gcaggtgacc ttcggcattg cggtgcgcat ggcggtgatg agcatactgg
     5161 caggtaacta aagcatgaaa atcgagatca agaatggccg cgtgatcgat ccggccaacg
     5221 gcatcgacgc gcagagaaac gtctacatcg ccgacggccg gattgccgcg gttggagaag
     5281 cacccgccgg attcacggcc gaccagacga tcgacgccgc cggcatgatc gtcgtgcccg
     5341 gcctggttga cctttctgcc cgcctgcgcg agcccggcta cgaatacaag gccacgctgg
     5401 aatcggaaat gcaggcggcg atgcagggcg gcgtgaccag tctgctgtgc ccgcccgata
     5461 ccgaccccgt tctggacgaa cccggcctgg tggagatgct caagcaccgc gcgcgcaacc
     5521 tgaaccaggc caacgtgtat ccgctcggcg cgctgacaat gggcctgcgc ggcgaggcgc
     5581 tgaccgagat ggccgaactg accgaggcgg gctgcatcgg ctttgcccag gccgagcagc
     5641 cgatcggcga caccaccgtg atgctgcgcg cgctgcagta tgcgaagacc ttcggctaca
     5701 ccgtctggct gcgtccgcaa gacccgcacc tgggccgcaa cggcaatgcg cacagcggcg
     5761 cggtggcgtc tcgcctgggg ctggccgggg tgccggtgat ggccgagacg atcgccctgt
     5821 acaccatctt cgaactggtg cgcgcgaccg gcgcccgcgt gcacttgtgc cgcatctcct
     5881 ccgccgccgg gctggagttg atgcgtgcgg cgaagaagga gggtctgccg gtcagctgcg
     5941 atgtcggcgt gcatcacatc cacctgaccg atctcgacat cggcttcttc gattcgaacg
     6001 cccgcctgac gcccccgctg cgtgagcaac gcgaccgcga cgcgattcgc gccgcactcg
     6061 ccgacggcac catcgatgcg atctgctccg accacacgcc ggtagacgac gatgaaaaac
     6121 tgctgccgtt cggcgaagcg acgcccggtg cgactggact ggaactgctg ctgccgctca
     6181 cgctcaagtg ggccggggag tgcctgggcg acaagggcct gtccacggcg attgccagga
     6241 tcacgtccga tgccgcgcgt gtggctggtc ttgcggccgg tgcgctgacg cgaggtgccg
     6301 cggccgatct ctgcgtgttc gatccgtacg cgcgctggaa ggtggaagcg aaggcgctcg
     6361 ccagccaggg caagcacacg cctttcatcg gatacgagct ggaagggcag gtgcgcgcga
     6421 cggtggtggc gggacgggtt gcgtatcagc gtgggtaagg gtgctgcttg cgagcgcaat
     6481 cgggcgacgg tgatgcgcct gaaagcgcgg gcccttccgc tcgcatgaga ctcttgcgcc
     6541 tcctctggct gatcctccac ctgttccgcg ggctggccac ctgcgcgctg atctttccct
     6601 tcgtcgacgt gcgcggccgc gagcaccata tccgccgctg gtcgcacgcg ctattgcgca
     6661 tctgccgtgt cgagctgcgc gtgatccgca acgacgaggc ggaagacctg ccgcgtgcgc
     6721 tcatcgtcgc caaccaccat tcctggctcg acatcttcgt gatcaacgcg atgcatccgt
     6781 gccggttcgt cgcgaagtca gagatccgca aatggcctct gatcggctgg ctgtgcgaac
     6841 aggtcggcac gatcttcatc gaacgcgggc ggctgcgcga cgtgcggcgc atctaccagg
     6901 gcctggtcga gagcctgcat gcgggcgagc atgtcgcgtt ctttccggaa ggaacggtct
     6961 ccgagcaggg cagcctgcac ccgttccacg ctaacctgtt cgaggcggcg attgaagcgg
     7021 cggtgccggt gcagccgttt gcattgcgct atctcgaccg ggcaggcaac tacgccgaat
     7081 cgatccgcta cgttgtcccg atcaccttcc cgcagagcct gtggatgatc ctcggcggcc
     7141 cgcccatcgt tgccgagctg agactgcttg cgcctatccc cacgccgcat ggcgcgcacc
     7201 ggcgcgacct tgcgaatgcg gcccgcgccg cgatcgcgcc ggcgctgggc atcccgaccg
     7261 cagaagttca gaccgagcca ggcgcctgat actgcgggca gttgacctgc accacgtgcc
     7321 ggtccgataa ccgcaccgcc gtaagcttgc cgccccacag gcagccggta tccaggctga
     7381 tcagattcgg ccgcagcacg aggccgagcg tggaccagtg accgaacacc acggtgaccg
     7441 cctcggtctt gcggcccggc acatcgaacc atggcatata gcccggcatc tccgtctcca
     7501 cacccttcga agtgagttcc atcgtgccgt ccgcgctgca gaagcgcagc cgcgtgagcg
     7561 cattgacgat gcagcgcaac cggtcggcgc cttctagatc gtctgaccag cgcgccggcg
     7621 tgttgccgta catgcgcttc agaaattcca cccagtccgg gccgcgcagc accgactcca
     7681 cttcctgcgc aagcgcaagc gtctgcgccg ccgtccactg cggcagcacg ccggcgtgca
     7741 cgaccaggtg gccgttgacc tcgagcgcca gcggctgctt gcgcagccag tcgagcagtt
     7801 cacggctgtc cgccgcctga agaatcggat ccagcgtgtc gctgcgatgc ggcgcgcgga
     7861 tatcgtttgc gaccgcgagc agatgcagat cgtgattgcc tagtacggtg cgggcccgct
     7921 cgcccagcgc gcgcacccgg cgcagggttt ccagcgaacg gggaccgcgg ttcacaagat
     7981 ctccgacgaa cacgaaggac gcgcccgcat cctcttcctc gatcttgcgc aggagcactt
     8041 ccagccgctc gccgcagccc tggatatcac cgatgacgta ggttgacatc aataaacgtt
     8101 gacgtgaaaa aaacaaggag cacgccgttt gttgaatgac aaaaggataa aaaaacccgt
     8161 gctctataaa agagtgtccc ttgtatgcag cccatgtatg taaccccatg tggtcgcacc
     8221 aacactcgct tatatcatgt taattccaat cattctttcc ggcggcgcgg gcacgcgcct
     8281 gtggccggtt tcgcgcgaag cgcaccccaa gcctttcatg cgtatcggag gcggcaagag
     8341 cctgattgca cagacctgcg agcgcgccca gactaccagc cccggcggca tgtccctggt
     8401 ggtgaccaat cgcgagtact acctgcgcac gcgcgaggaa ctcgccaagc aggatttcat
     8461 gccgcactac ctgctcgagc cggtgggccg caataccgcg cccgccgtcg ctgccgcggc
     8521 gctgtgggcg ctcaggcagg atgcggatgc ctgcctgctc gtgatgcccg ccgatcacct
     8581 gatcaccgac accgccgcct tccagcaggc cgcgaagtac gctgaaatgc tcgcgcgtga
     8641 cggcttcctt gtct
//
LOCUS       DATLGF010000064         2956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_50023,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000064 DATLGF010000000
VERSION     DATLGF010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50023"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VM406_02010"
     CDS             complement(<1..221)
                     /locus_tag="VM406_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74765.1"
                     /translation="MRPEPRKRSDYLHFHTISTRWMDNDAYGHVNNVVYYSWFDTVVN
                     RFLVAGGGHVFVFTEDADLVAYAGVPERG"
     gene            complement(211..1119)
                     /locus_tag="VM406_02015"
     CDS             complement(211..1119)
                     /locus_tag="VM406_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVL74766.1"
                     /translation="MSETGLSRTSSHTGRTAIPFVFVLIWSTGFVVAKFGLPYAPPLT
                     FLLLRFAGVLIVLLPLIWILRLPWPQGQIHHIAVAGVLLQAGYLGGVWYAIWLGMPAG
                     LSALIVGMQPVLTALFASFVGESVDRRQWTGLGFGLAGVALVVADKVSLTGLSWQSIV
                     LCTFALLAITAGTLYQKRYCASFDLRTGTVIQFGASFLVMLPLALAFEGLDPAFSAVQ
                     WTPQFIGAWLWAVLALSIGAIFLLFALIRRSAATEVTTLMYLTPPTTAVMAWLMFGEA
                     FSVRAMVGMALAVTGVALVVKKQYET"
     gene            complement(1116..2030)
                     /locus_tag="VM406_02020"
     CDS             complement(1116..2030)
                     /locus_tag="VM406_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HVL74767.1"
                     /translation="MKAFYSDHFVLPLPQGHRFPMQKYRMVRDAVLHQLAMVELSPAP
                     AASDGVLALAHHPEYIRRIAEGATSDEEQKAIGFPWSPQMVERARRSVGATIAACRAA
                     LDHGAGANLAGGTHHAYADCGGGFCVFNDAAVAARLMQAERRAARVAIIDLDVHQGNG
                     TAAILSRDDSVFTLSLHGASNYPFDKECSDLDVPLADGTGDAEYLHALGDALDELARR
                     FAPQLIIYLAGADPHEGDRLGRLKLTTEGLRRRDAMVFEFAARRRLPVAVTMAGGYGR
                     AIEDTVAVHVQTLAEAARYAAAWEGKLR"
     gene            2264..2827
                     /locus_tag="VM406_02025"
     CDS             2264..2827
                     /locus_tag="VM406_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HVL74768.1"
                     /translation="MFAIPEQFSAASKASLEAQLALLSSLTSKTFESVEKVVELNLEA
                     ARASLEESAANARQLLAAKDAQEWMSLAAAQAQPNAEKALSYGRHLASIASGVQAEFH
                     KAAEAQVAESSRKLLELVEEVTKNAPGGSENAVALLKSAIGNASAGYEQMTKSTKQAV
                     ETLEANLNNAVTQFTQPVAKAARAAKK"
BASE COUNT          607 a          955 c          944 g          450 t
ORIGIN      
        1 tggccacgct cgggaacacc agcgtacgct acgaggtcgg catcttccgt gaagacgaag
       61 acatgcccgc cgcccgcgac caggaagcga ttgaccaccg tgtcgaacca gctgtagtag
      121 acgacgttgt tgacgtgccc ataggcgtcg ttatccatcc agcgcgtgct gatcgtgtgg
      181 aaatgcagat agtcgctgcg cttgcggggt tcaggtctca tattgttttt tcaccacgag
      241 tgctacgcca gtgacagcca gcgccatgcc gaccatcgca cgcacgctga acgcttcccc
      301 gaacatcagc caggccatga cggccgtggt cggcggggtc aggtacatca atgttgtgac
      361 ctcggttgct gcgctcctgc ggatcagcgc gaacagcagg aagatcgcgc cgatcgacaa
      421 cgcaagcacc gcccacagcc acgccccgat gaactggggc gtccactgca cggcggagaa
      481 agcgggatcg aggccttcga acgccagcgc cagcggcagc atgaccagga acgaggcacc
      541 gaactggatc acggttccgg tgcgcaggtc gaaactcgcg caatagcgct tctggtatag
      601 cgtgccggcg gtgatcgcca gcaaggcaaa tgtgcacagc acaatgctct gccatgataa
      661 gccggtcagc gataccttgt cggcgaccac caaggcgacg ccggcgagac cgaagccaag
      721 gcccgtccat tgcctgcgat cgaccgactc gccgacgaac gaagcgaaca gcgccgtcag
      781 cacaggctgc atgccgacga tgagcgccga caggccggcc ggcatgccaa gccagatcgc
      841 ataccagacg ccgcccagat agccggcctg cagcagcacg ccggccacgg cgatgtgatg
      901 aatctggcct tgcggccacg gcaggcgcag tatccagatc agcggcagca gcacaatcag
      961 gacgccggca aagcgcagca gcaggaaggt cagcggaggc gcatacggca ggccgaactt
     1021 cgcgaccacg aagcccgtgc tccagatcag cacgaacacg aacgggatgg cggtacggcc
     1081 ggtatggctc gacgtccggc tcagtccggt ttcgctcatc gcagctttcc ttcccaggcc
     1141 gccgcgtagc gtgcggcctc ggcgagggtc tggacatgca ccgcgacggt gtcctcgatc
     1201 gcgcgaccgt agccgccggc catcgtcacc gccaccggca agcgacgacg cgcggcgaac
     1261 tcgaacacca tggcatcgcg ccggcgcagg ccttccgtcg tgagcttgag ccgtccgagg
     1321 cgatcgcctt cgtgcggatc ggcgccggcc aggtagatga tgagctgcgg cgcgaaacga
     1381 cgcgccagct cgtccagcgc atcgccgagg gcgtgcagat attcggcgtc gccggtgccg
     1441 tcggcgagcg gcacgtcaag gtcgctgcat tccttgtcga acgggtagtt cgacgcgccg
     1501 tgcagcgaga gcgtaaacac actgtcgtcg cgggagagga tggcggcggt gccattgccc
     1561 tggtggacgt cgaggtcgat gatcgccacc cgcgccgcgc gccgctcggc ctgcatcagg
     1621 cgggccgcca ctgcagcgtc gttgaaaacg caaaagccgc cgccgcaatc ggcataggca
     1681 tggtgcgtac ctccggcgag attcgcgccg gcgccatggt caagcgcggc ccggcaggcg
     1741 gcgatggtcg cgcccaccga gcggcgcgcg cgttcaacca tctgcggcga ccacggaaaa
     1801 ccgatcgcct tctgctcctc gtcgcttgtc gcgccttccg cgatacggcg tatgtactcc
     1861 ggatgatggg ccagcgccag caccccatcg cttgccgccg gcgccggact gagttcaacc
     1921 atggcgagct ggtgcagaac ggcgtcgcgc accatgcggt acttctgcat cgggaatcgg
     1981 tggccctgcg gaagcggcag cacgaaatgg tcgctgtaga aggccttcaa ggggggaatt
     2041 gacgtatacg taaagtagcg gcgagtgtag cacgggaggg cttgccaata tgctcctgtc
     2101 ctgcgcggca cgttgacaca cggcaacgtg gcgcatttta ttgcgaagca caaaaaatcg
     2161 gttgacacca ggcgtgatca acgtagaatg cgcgattgtt gcgatgcaca aaacgcctcg
     2221 cattttttgc gccattcctg gaaccccctc cgtaggagac gaaatgtttg cgatccccga
     2281 acagttttcc gccgcttcca aagccagcct cgaagcccag ctcgccctgc tgagcagcct
     2341 gaccagcaag accttcgaga gcgtggaaaa ggttgtcgaa ttgaatctcg aagccgccag
     2401 ggcctcgctg gaagagtccg ccgcgaatgc acgccagctg ctcgccgcca aggatgcgca
     2461 ggaatggatg agcctggctg ccgcgcaggc tcagccgaac gccgaaaagg cgctgtctta
     2521 cggccgccac ctggccagca ttgccagcgg cgtgcaggcc gaattccaca aggccgccga
     2581 agcgcaggtt gccgaatcca gccgcaagct gctggaactg gttgaggaag tcacgaagaa
     2641 cgcaccgggc ggctctgaaa acgccgtcgc cctgctgaag tcggcaatcg gcaacgccag
     2701 cgccggctac gagcagatga ccaaatccac caagcaggcg gtcgagacgt tggaggccaa
     2761 tctgaacaat gcggtgaccc agttcaccca gccggtggcc aaggcagcgc gcgcggcaaa
     2821 gaagtaattg gtgcgagcgg cgctgggcgc cgcctccgcc gaaaaggccc cgccgctgcg
     2881 gggctttttt tcgtccgttc ggcggtacca accagaaaca aaaagcgccc cgaagggcgc
     2941 cttgcacgtg atgaac
//
LOCUS       DATLGF010000065         7673 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_19359,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000065 DATLGF010000000
VERSION     DATLGF010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 7673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7673
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19359"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VM406_02030"
     CDS             complement(<1..718)
                     /locus_tag="VM406_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HVL74769.1"
                     /translation="MSLQEVKVPDIGDFSEVEVIELLVKPGDTIKVDQSLATVESDKA
                     SMEIPSSHAGVVKEVKIKVGDKVSEGSLLLVVESEAGAAAPAPAPAAAPAPAPAAAPA
                     PAAASAPAPAGAPEEVFVPDIGDFQDVEVIELMVKPGDAVKVDQSLMTVESDKASMEI
                     PSTHAGVVQDIKVKVGDKVSKGSLILTLAGAAGAPAPAPSAAAPAPAATPPAPAPAAP
                     PAAARVAPTAALEPVERNGVK"
     gene            complement(973..3669)
                     /gene="aceE"
                     /locus_tag="VM406_02035"
     CDS             complement(973..3669)
                     /gene="aceE"
                     /locus_tag="VM406_02035"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HVL74770.1"
                     /translation="MSAQLDQVLAQAANDPDVMETQEWLDALEAVIDTEGPERAHYLM
                     ERMVDLARRRGAHIPFSANTAYVNTIPAHLEEHCPGNLEYEERLRSWMRWNAMAMVVK
                     ANRADGDLGGHISSFASLANMLGIGFNHFWHAPTEEHGGDLLYIQGHSSPGIYARAFL
                     EGRLSEDQMLNFRREVDGKGLSSYPHPKLMPDFWQFPTVSMGLGPLMAIYQARFLKYL
                     HARSIAKTDNRKVWAFLGDGEMDEPESMGAIGVAGREQLDNLVFVVNCNLQRLDGPVR
                     GNGKIIQELEGEFRGAGWNVIKVIWGSYWDELLARDKEGILQRVMMETVDGEYQNYKA
                     KDGAYVRKHFFGKHPKLLEMVSRMSDDDIWRLNRGGHDPHKIYAAFKRAQEHTGQPTV
                     LLVKTIKGFGMGKSGEARNTAHQTKKLDDAAVREMRDRFNIPIPDDKLPEIPFFKPSD
                     DAPEMKYLHERRKALGGYLPQRRVKAEESLPVPPLSAFQAVLDPTAEGREISTTQAYV
                     RIITALLRDANLGQRVVPILVDEARTFGMEGLFRQIGIYSQQGQLYEPVDRDQVMYYR
                     EDKAGQILQEGINEAGAMSSWIAAATSYSTNNRIMIPFYIFYSMFGLQRVGDLAWAAG
                     DMRARGFLLGGTAGRTTLNGEGLQHEDGHSHVMASTIPNCIPYDPTFAHEVAVIIHDG
                     LKRMVENQEDVFYYITLMNENYSHPGLKPGQEEGILKGLYLLQQGDANAKKRVQLMGS
                     GTILREAIFAAELLQNDWGIAADVWSAPSFTLLARDGQDADRWNLVHPLEQPRLPYVT
                     QQLQGTSGPIVATSDYMRTFAEQIRAFMPKGRTYKVLGTDGFGRSDSRAKLREFFEVN
                     RYYVTVAALKALAEDGTIEASVVADAIRKYGIDPNKPNPVTL"
     gene            3916..4761
                     /gene="folD"
                     /locus_tag="VM406_02040"
     CDS             3916..4761
                     /gene="folD"
                     /locus_tag="VM406_02040"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HVL74771.1"
                     /translation="MPAQLINGTLLSQQLRADVARRAAALTAKGKQPGLAVVLVGDNP
                     ASQVYVRNKVKACEDNGIHSVFEKYEADLSEEALLARIDALNSDPKIHGILVQLPLPA
                     HIDAHKVIEAIAPEKDVDGFHISNAGLLMTGQPLFRPCTPYGVMKMLESIDYPVRGAH
                     AVVVGASNIVGKPQAMLLLQAGATVTICNSKTRDLGHHTRDADILVVATGKRNIVTAD
                     MIKPGAVVIDVGMNRDDEGKLCGDVDFANAKEVAGWITPVPGGVGPMTITMLLVNTIE
                     AAERT"
     gene            4761..6827
                     /locus_tag="VM406_02045"
     CDS             4761..6827
                     /locus_tag="VM406_02045"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HVL74772.1"
                     /translation="MTRHTNPLLDFSDLPRFEAVKPEHVGPAVDKLLAENRTVVAQLE
                     QPQDHVTWENFVAPLENATERLSRAWGVVGHLNSVMDSPELRAAYNENLPKVTEFWTG
                     LGQNLALFRKYKAIRESAVFDTLSPARKRIIENALRDFRLGGAELPDDKKARFAAIQE
                     KLAALRTKFSENVLDATNDFTLFIEDEADLAGLPQDTLHGARAAAERDGKPGYKFTLH
                     FPSYFPILQYADKRALREAIYRANVTKASELGAKAEWDNTHNIEEILKLRAEEARLLG
                     FSSFAEVSLVAKMADTPQQVIAFLEDLARRARPFAEKDLAELRDFARAELGLETLEAW
                     DVAYASEKLRQKRYAFSEQEVKQYFPEPKVIEGLFRVVQSLFGVSVRPDAAACWHKDV
                     KFFRIEKDGELVGQFYLDLYARHGKRGGAWMNDARGRRRLVSHTQTPVAYLVCNFSEP
                     TVVNGKTRPALFTHDEVITLFHEFGHGLHHMLTRVDELGVAGIAGVEWDAVELPSQFM
                     ENFCWEWEVLQQMTAHVDTGEPLPRPLYDKMIAAKNFQSGLQTLRQVEFALFDMHLHF
                     DHDPNGSQPVQQVLDEVRQRVAVMIPPSFNRFQNSFGHIFAGGYAAGYYSYKWAEVLS
                     ADAYSAFEEAGEKTGSVLSVETGLKFLTEVLAAGGARSALESFKAFRGREPKIDALLR
                     HSGMAA"
     gene            6939..7565
                     /locus_tag="VM406_02050"
     CDS             6939..7565
                     /locus_tag="VM406_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HVL74773.1"
                     /translation="MKHLQPLLPVLLVLCAGAAHAQLYKWVGPDGKVTYSDVPPPAGA
                     KAEARAAPAATGGAASDLPYELAQAARNHPVVLYTTADCLPCDDGRKLLNARGIPFRE
                     RTVTSNEDIAQVRQLGGDTNLPLLTVGRARERGYEPNAWNNALTIAGYPAASRLPRNY
                     RQPAPEPAAPRVEPVAPKQEEPAAAPAPATPPTELPPATGNAPPGFRF"
     gene            7565..>7673
                     /locus_tag="VM406_02055"
     CDS             7565..>7673
                     /locus_tag="VM406_02055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HVL74774.1"
                     /translation="MTRIDFHTDLPDKIAYCCRLARKAYQSGARVVVVAP"
BASE COUNT         1315 a         2512 c         2470 g         1376 t
ORIGIN      
        1 gtttcacgcc gttccgttcg accggctcca gcgcggcggt gggcgcaacg cgggcggcgg
       61 ccggcggcgc ggcgggcgcc ggcgccggcg gggtcgcggc aggggccgga gccgcagcgg
      121 aaggcgcggg tgcaggcgcg ccagccgcac cggccagcgt caggatcaac gaacccttgg
      181 ataccttgtc gcccaccttg accttgatgt cctgcacgac gccggcatgg gtcgacggaa
      241 tttccatgct ggccttgtcc gattccaccg tcatcagcga ctggtcgacc ttcacggcat
      301 cgcccggctt gaccatcagc tcgatgacct ccacgtcctg gaaatcaccg atatcgggaa
      361 cgaacacctc ctccggcgcg cccgcgggcg cgggcgcgga ggcggcggcc ggggccggcg
      421 cggctgccgg tgccggtgcc ggtgcggctg ccggggcagg cgccggcgcc gcggcgccag
      481 cttcggattc caccaccagc aacagcgaac cctcggagac cttgtcgccg accttgatct
      541 tcacttcctt gacaacgccg gcatggctgg acgggatctc catgctggcc ttgtcggatt
      601 cgacggtcgc gagcgactgg tccaccttga tcgtgtcgcc gggcttgacc agcaactcga
      661 tcacctccac ttcactgaaa tcgccgatat ccgggacttt gacttcctgc aagctcatct
      721 tgtgtgctcc gttttttgtt ctatcaccag cccgcccctt cccctgccgg ggcgagggtc
      781 gggatggggt actgagtccg agcgaacgac tttgccaggc ccaagctcgc ccccctccct
      841 ggcccgcccg cctcacagct tgcaagctgc tcggatttgc gtggcgcaaa gcatgctttg
      901 cgaaaccccg cgctcatccc cctgtatgga gaaaggaacc ggccgtcgat cttgaaagag
      961 aaatctctat gcttacagcg ttaccggatt gggcttgttc ggatcgatgc cgtacttccg
     1021 gattgcgtcc gccaccaccg aagcttcgat cgtaccgtct tcggcgagcg ccttcagcgc
     1081 ggcgaccgtc acgtagtaac gatttacctc aaagaattcg cgcagcttgg cccggctgtc
     1141 ggaacggccg aagccgtcgg tacccaagac cttgtaggtg cggcccttcg gcatgaaggc
     1201 gcgaatctgc tcggcgaacg tgcgcatgta gtcggaagtg gcgacgatcg gcccggacgt
     1261 gccctgcagc tgctgcgtga cgtacggcag gcgcggctgc tcgagcgggt ggacgaggtt
     1321 ccagcggtcg gcgtcctggc cgtcgcgcgc cagcagggta aacgaaggcg cggaccagac
     1381 gtcggcggcg atgccccagt cgttctgcag cagttcggcg gcgaagatcg cctcgcgcag
     1441 gatcgtgccg gagcccatca gctgcacccg cttcttcgcg tttgcatcgc cctgctgcag
     1501 caggtacagg cccttcagga tgccctcttc ctggcccggc ttcaagcccg gatggctgta
     1561 gttctcattc atcagggtga tgtaatagaa cacatcctcc tggttctcga ccatgcgctt
     1621 caagccgtca tggatgatca ccgcgacttc gtgcgcgaag gtcgggtcgt acggaatgca
     1681 gttcgggatc gtcgacgcca tgacgtggct gtggccgtcc tcgtgctgca ggccctcgcc
     1741 gttcagggtc gtgcggccgg cggtgccgcc gagcaggaag ccgcgcgcgc gcatgtcgcc
     1801 agcggcccag gccaggtcgc cgacgcgctg cagcccgaac atcgagtaga agatgtagaa
     1861 cgggatcatg atgcggttgt tcgtcgaata cgacgtcgcc gcggcgatcc aggagctcat
     1921 cgcgcccgct tcgttgatgc cttcctgcaa gatctggccg gccttgtcct cgcggtagta
     1981 catcacctgg tcgcggtcga ccggctcgta cagctggccc tgctggctgt agatgccgat
     2041 ctggcggaac aggccttcca tgccgaaggt gcgcgcttcg tcgaccagga tcggcacgac
     2101 gcgctggccg aggttcgcat cgcgcaggag ggctgtgatg atgcgcacgt acgcctgcgt
     2161 cgtcgagatt tcgcggcctt cggcggtcgg atcgagcacc gcctggaacg ccgacagcgg
     2221 cggcaccggc aggctttcct cggccttcac gcggcgctgc ggcaggtagc cgcctagggc
     2281 cttgcggcgc tcgtgcaggt acttcatttc cggcgcatcg tcggacggct tgaagaacgg
     2341 gatttccggc agcttgtcgt ccggaatcgg gatgttgaag cggtcgcgca tctcgcgcac
     2401 ggcggcgtcg tcgagcttct tggtctggtg cgcggtgttg cgcgcctcgc ccgacttgcc
     2461 catgccgaag cccttgatgg tcttgaccag cagcacggtc ggctggccgg tgtgctcctg
     2521 cgcccgtttg aacgcggcgt agatcttgtg cggatcatgg ccgccgcggt tcaggcgcca
     2581 gatgtcgtcg tccgacatgc gcgacaccat ctccaggagc ttcggatgct tgccgaagaa
     2641 gtgcttgcgc acgtacgcgc cgtccttggc cttgtagttc tggtattcgc cgtcgacggt
     2701 ttccatcatc acgcgctgca ggatgccttc cttgtcgcgc gcgagcagct cgtcccagta
     2761 gctgccccag atgaccttga tcacgttcca gccggcgccg cggaactcgc cttccagttc
     2821 ctggatgatc ttgccgttgc cgcgcaccgg gccgtccagg cgctgcaggt tgcagttgac
     2881 gacgaacacc aggttgtcga gctgctcgcg tccggccacg ccgatcgcgc ccatcgattc
     2941 cggctcgtcc atttcaccgt caccaaggaa ggcccagacc ttgcgattgt cggtcttggc
     3001 gatgctgcgc gcgtgcagat acttcaggaa gcgcgcctgg tagatcgcca tcagcgggcc
     3061 gaggcccatc gacaccgtcg ggaactgcca gaaatcgggc atcagcttcg ggtgcgggta
     3121 ggacgacagg cccttgccgt ctacctcgcg gcggaagttc agcatctggt cttcggacag
     3181 gcgcccctcc aggaaggcgc gagcgtagat gccgggagag gaatggccct ggatgtacag
     3241 caggtcgccg ccgtgctctt cggtcggcgc gtgccagaag tggttgaagc cgatgcccag
     3301 catgttggcc agcgacgcaa acgaggagat gtggccgccg aggtccccat cggcacggtt
     3361 ggccttcacc accatcgcca tggcgttcca gcgcatccag gagcgcaggc gctcctcata
     3421 ttcgaggttg ccgggacagt gttcttccag gtgggcgggg atcgtgttga cgtaggcggt
     3481 gttggccgag aacgggatat gggcgccgcg gcggcgggcc agatcgacca tgcgctccat
     3541 caggtaatgc gcacgctcgg gaccctcggt gtcgatcacg gcctcaaggg cgtcgagcca
     3601 ttcctgcgtt tccattacat cgggatcgtt ggcggcttgc gccaatacct ggtcaagctg
     3661 ggcggacatc gtgcgtctcc tctggtgggg gctccgagcg ggtctcggcg tggggatttg
     3721 gacaagatct ggccggcgtg gtgcgcggtg aaaagagttt aacagcagtt tttctttttt
     3781 caaatagcga tacgtctttt cataatgcgg gattttgtgg tgcagtgcag cagcgcggct
     3841 tccgccgctc cagacgggac gcccgcttcg cccaccttat aattccgctt ttcccatccc
     3901 gcccgtctac ctgccatgcc cgcccaactc atcaacggaa ccctcctctc ccaacaattg
     3961 cgcgctgatg tcgcacggcg cgcggccgcg ctcactgcca agggcaagca gcccggactg
     4021 gcggtcgtgc tggtcggcga caatcccgcg tcccaggtct atgtgcgcaa caaggtcaag
     4081 gcctgcgaag acaacggcat tcattcggtg ttcgagaaat acgaggccga cctgagcgag
     4141 gaggcgctct tggcgcgcat cgatgcgctc aacagcgatc cgaagatcca cggcatcctg
     4201 gtgcaacttc cgctgccggc gcacatcgat gcacacaagg tgatcgaggc gatcgcgccg
     4261 gagaaggatg tcgacggctt ccatatcagc aacgccggcc tgctgatgac cggccagccg
     4321 ctgttccgtc cgtgcacgcc gtatggggtg atgaagatgc tcgaatcgat cgactacccg
     4381 gtgcgcggcg cgcatgcggt cgtggtgggc gcgtcgaaca tcgtcggcaa gccgcaggcg
     4441 atgctgctct tgcaggcggg tgcgaccgtc accatctgca actccaagac ccgggacctc
     4501 ggccaccaca cccgtgatgc cgacatcctc gtggtcgcca ccggcaagcg caacatcgtc
     4561 accgccgata tgatcaaacc aggtgccgtg gtgatcgacg tcggcatgaa ccgcgacgac
     4621 gaaggcaagc tgtgcggcga tgtcgatttc gccaatgcca aggaagtcgc cggctggatc
     4681 acgcctgttc cgggcggggt cggaccgatg acgatcacga tgctgctagt caacacaatc
     4741 gaggccgctg aaagaacctg atgaccagac acacgaatcc tcttctcgat ttctccgacc
     4801 tgccgcgatt tgaggcggtc aagcccgaac acgtcggccc cgcggtcgac aaactcctgg
     4861 ccgaaaaccg cacggtggtc gcgcagctgg aacagccgca agaccacgtc acctgggaga
     4921 acttcgtcgc cccgctggaa aacgcaaccg agcgcttgag ccgcgcctgg ggtgtggtcg
     4981 ggcacctgaa ctcggtgatg gattcgccgg aactgcgcgc cgcgtacaac gagaacctcc
     5041 ccaaggtcac cgagttctgg accgggttgg gccagaacct cgcgctgttc cgcaaataca
     5101 aggcgattcg cgaaagcgcg gtgttcgaca cgctcagccc ggcccgcaag cggatcatcg
     5161 agaacgcgct gcgcgatttc cgcctcggcg gcgcggagct gcccgacgac aagaaggcgc
     5221 gcttcgccgc aatccaggaa aagctggccg cgctgcgcac gaagttctcg gaaaacgtgc
     5281 tggatgcgac caacgacttc acccttttca tcgaggacga ggccgacctc gccggcctgc
     5341 cgcaggacac gctgcatggc gcgcgcgcag ccgccgagcg cgacggcaag cccggttaca
     5401 agttcacgct gcacttcccg tcctatttcc cgatcctgca atacgcggac aagcgcgcgc
     5461 tgcgcgaggc gatctaccgg gccaacgtga ccaaggcgtc cgaactcggc gccaaggcgg
     5521 agtgggataa cacgcacaat atcgaagaga tcctgaagct gcgcgcggaa gaggcgcggc
     5581 tgctgggctt ttccagcttc gcggaagtgt cgctggtggc gaagatggcc gatacgccgc
     5641 agcaggtgat cgccttcctg gaagacctgg cgcgccgcgc gcgcccgttc gccgagaaag
     5701 atctggccga gctgcgcgat ttcgcgcgcg cggagctcgg cctggagacg ctggaagcct
     5761 gggatgtcgc ctatgcgtcg gaaaagctgc gccagaagcg ctacgcgttc tccgagcagg
     5821 aggtcaagca gtacttcccg gagccgaagg tgatcgaggg cctgttccgc gtcgtgcagt
     5881 cgctgttcgg cgtgagcgtc aggccggatg ctgcggcgtg ctggcacaag gacgtgaagt
     5941 tcttccgcat cgagaaggac ggcgagctgg tcggccagtt ctacctggac ctgtatgccc
     6001 gccacggcaa gcgcggcggc gcgtggatga acgacgcgcg cggacgccgc cgtctcgttt
     6061 cgcataccca gacgccggtt gcttatctgg tttgcaattt ttccgagccg accgtcgtca
     6121 acgggaagac ccgcccggcg ttgttcacgc acgacgaagt catcacgctg ttccacgagt
     6181 ttggacacgg gctgcaccac atgctgaccc gggtcgatga actgggcgtg gccggcattg
     6241 ccggcgtcga atgggatgcg gtcgaactgc cgtcccagtt catggagaac ttctgctggg
     6301 agtgggaagt gctgcagcag atgaccgctc acgtggatac cggcgaaccg ctgccgcgcc
     6361 cgctgtacga caagatgatc gcggcgaaga atttccagtc cggcttgcag acgctgcgcc
     6421 aggtcgagtt cgcgctgttc gacatgcatt tgcatttcga ccacgatccg aacggcagcc
     6481 aaccggtcca gcaggtgctc gacgaagtcc ggcagcgtgt cgccgtgatg atcccgccgt
     6541 ctttcaatcg gtttcagaat tccttcgggc acatctttgc cggcgggtat gcggcgggat
     6601 actatagcta caagtgggcc gaagtcctgt cggccgacgc ctacagcgcc ttcgaggagg
     6661 cgggagagaa gactggcagc gtgctgtcgg tcgaaaccgg cctcaagttt ttgaccgaag
     6721 tactggcggc gggcggcgca cggtccgcgc tggagtcgtt caaggccttc cgcggccgcg
     6781 aaccgaaaat cgatgccctg ctgcgccaca gcggcatggc cgcctagtgg tccggcacac
     6841 tccagatttc gttttactcc gagttgatcg aaggcgagcg caaccggcgg aaacgaaaaa
     6901 tgggtgtggc gggccactaa cccctcttgg aatatcgcat gaagcacctg cagccgttgt
     6961 tgcctgtctt gctcgtcctg tgcgccggcg cggcgcacgc gcaactgtac aaatgggtcg
     7021 gtcccgacgg caaggtcacg tacagtgacg tgcccccgcc ggccggcgcg aaagccgaag
     7081 cccgcgccgc tcctgccgcg acgggtggcg ccgcaagcga tctgccgtac gagctcgccc
     7141 aggcggcacg taatcatccc gtcgtgctct acacgaccgc ggattgcctc ccgtgcgatg
     7201 acggccgcaa gctgcttaac gcgcgcggca tcccgttccg cgaacggacc gtgacgagca
     7261 acgaggacat cgcgcaggta cgccagctcg gcggcgacac caacctcccg ctgctgaccg
     7321 tcggtcgcgc gcgcgaacgc ggctacgagc ccaatgcctg gaacaacgcg ctgacgatcg
     7381 ccggctatcc tgccgccagc cgcctgcccc gcaactatcg tcagcccgcc ccggaaccgg
     7441 cggcgccgcg cgtcgagccg gtcgcgccga aacaggaaga gcctgccgcc gcgcccgcgc
     7501 cggcgacccc accgaccgaa ttgccaccgg ccaccggcaa cgcgccgccc ggtttccgct
     7561 tctgatgacc cgcatcgatt tccataccga tctgcccgac aagatcgcct actgctgccg
     7621 cctggcgcgc aaggcctacc agagcggagc ccgcgtggtc gtcgtggcgc ccg
//
LOCUS       DATLGF010000066         4115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_50096,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000066 DATLGF010000000
VERSION     DATLGF010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50096"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(335..1540)
                     /locus_tag="VM406_02060"
     CDS             complement(335..1540)
                     /locus_tag="VM406_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010811703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVL74775.1"
                     /translation="MTERYVKKREAILHAAAQLFNQHGIKGATLSDVAGSVGLSTNSI
                     TYYYKKKEDLVVACLLRSIEVISGICDEAVRGATPELRVRAFIRLLFARLASIGREQA
                     PELMSFRDVQMLGSPHAEVIFSAYTDLFRRARQLLVDGRPPLEQRAGINARCHLLLTL
                     GVTSLRWCARYDPDDYELVGEEMADMLIGGLCAGGMRWHPVALDEALAHPPLTVDSTQ
                     DAFLRAATALINELGYRGASVDRISARLNVTKGSFYHHNPNKEDLISSCFARTFAVIR
                     QAQMTARHGPGSGWDKLCALSRALVRYQFSEHGPLLRMSAWSELPEDLRAQTLHAVNQ
                     IELRYASFVVEGMKDGSIRPVDQTVAALLVSNMVNAAVVLDRWIPDVSADDAVELFAR
                     PLFVGILAQ"
     gene            complement(1654..2763)
                     /locus_tag="VM406_02065"
     CDS             complement(1654..2763)
                     /locus_tag="VM406_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HVL74776.1"
                     /translation="MEQRAKAVICRELNQPVVVEDIIVEAPGRGEVMIKLAACGVCHS
                     DLSATNGTIPFPPPVILGHEGAGRVVAVGEGVVDLSVGDHVLSSFVSMCGRCRYCQTG
                     RPQLCDQAARAAYTLPDGTVRTRDLSGNRLNVFSGCGVMAEYATLHVDNVVRIDNDVA
                     LDKAALIGCGVMTGVGAAVNTARVDAGSIAVVFGCGGVGLNAIQGCAIAGARMIVAVD
                     TSDAKLDMAREFGATHVVNGTAEENVVKRILKLTDGGADYAFECVGHGEIAAQAYGVL
                     RKGGTAVVVGVAAPKDLTTIRTASLTFGEKTLTGSYFGSARPREDFPRLLGLYSVGRL
                     KLDELITRTYTIAEAPQAFADMQAGRNARGVIVFA"
     gene            complement(2927..4084)
                     /locus_tag="VM406_02070"
     CDS             complement(2927..4084)
                     /locus_tag="VM406_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL74777.1"
                     /translation="MKKHAITAALAVGLSFAGAAMAQEVVKIGFSGPLSGGAALYGKN
                     VLSGMEMAVQEINAKGLEVGGKKYKLEIVSLDDKYNPSETGINVQRLIQQHKTPAVLV
                     PHSGGIFAVQAFNERSNVLLLAYTSLPQTTARGNKLTIRIPPEFTGYIEPFTKHAMSK
                     FGKNVAIANADHDYAKAWTAAFKPAWEAAGGKVVAENPMSYNKATDFYSGVSRVLQAK
                     PDVLFIGGASEPTALVVKQARELGFKGGFIIIDQAKMDEMAKVIGGYGPIEGSIGVLP
                     VIEDSRQQTQSWVERFRKMNPGRDPSSEVSLNYTAVHATALAMQLAGTVDDPAKIRAQ
                     MDRAFKELPAEHNPNDINGVDANGGSLANTYVATVENGKVRQVSLRDLGAK"
BASE COUNT          669 a         1366 c         1339 g          741 t
ORIGIN      
        1 tccttctagg gggaggccta ggggcgcccg gcgtagggag ggggtgcgaa actcgcgcgg
       61 gcgaatctgc cattgcaagc tccgtacccc catcccagcc ttccccctgg aagggggaag
      121 gagtcagttc atcacgacca agtcgcgcgt cactcttcca ggtggtagag ccaacagcat
      181 caaggcgttt gccaaagcgg caagtacttg ttccggacag tagtgcgcct gcgcgggaac
      241 gaaaaagcca gagccggtgg atctgccgtg ctttgcacgc gccttgcgcg acgacgacgc
      301 cgcccgaaca cagccgcaac cgctggacaa caagctactg cgccaggatc ccgacaaaca
      361 gcggccgcgc gaacaattcc accgcgtcgt cggcgctcac gtccggaatc caccggtcga
      421 gcacgaccgc ggcattgacc atgttcgaca ccaggagtgc ggccaccgtc tggtcgaccg
      481 gacggatcga accgtccttc attccctcga ccacgaagct cgcgtaacgc agctcgatct
      541 gattgaccgc gtgcagcgtc tgcgcgcgca ggtcctcggg cagctcgctc caggccgaca
      601 ttcgcaagag cggcccatgc tcggaaaact ggtagcgcac cagcgcgcgc gacagcgcgc
      661 acagcttgtc ccagccgctt cccgggccgt ggcgggccgt catctgcgcc tggcgaatca
      721 cggcgaaagt gcgcgcaaag caggaggaga tcaggtcctc cttgttcggg ttgtgatggt
      781 agaacgatcc cttggtgacg ttcagccggg ccgagatgcg gtccaccgac gcgccgcggt
      841 agccgagttc attgatcagc gcggtggccg cgcgcaggaa cgcgtcctgg gtgctgtcga
      901 cggtcagcgg cggatgcgcg agcgcctcgt ccagcgcgac cgggtgccag cgcattccac
      961 ccgcgcacag tccgccgatg agcatgtccg ccatctcctc gccgaccagt tcgtagtcgt
     1021 ccggatcgta gcgcgcgcac cagcgcaacg aggtgacgcc gagcgtgagc agcaggtggc
     1081 agcgcgcgtt gatgccggcg cgctgctcga gcggcggccg gccgtcgacc agcagctggc
     1141 gcgcgcggcg aaacagatcg gtatacgcgg agaagatcac ttcagcgtgc gggctgccca
     1201 gcatctgcac gtcgcgaaag ctcatcagct cgggcgcctg ttcgcgtccg atgctggcca
     1261 ggcgggcaaa cagcaggcgg atgaacgcac gtacccgcag ttccggcgtc gcgccgcgca
     1321 ccgcctcgtc acaaatgccg ctgatcacct cgatcgaacg caacaggcag gcgacgacga
     1381 ggtcttcctt tttcttgtag tagtaggtga tgctgttggt cgacaggccg acgctcccgg
     1441 ccacgtccga cagcgtcgcg cccttgatgc cgtgctggtt gaacagctgc gccgccgcgt
     1501 gcaggatcgc ctcgcgcttc ttgacatagc gctcggtcat gcggcttgcc tgcacgtgct
     1561 cttccatacg tcccgtttta tttatatgag gaaggcggcg attatactgc ccggcccagg
     1621 gcgaacgagg ctcgtcgcca gggcgacgcc ttgttatgcg aacacgatca cgccgcgggc
     1681 gttccgcccc gcctgcatgt cggcaaacgc ctgcggcgcc tcggcgatcg tgtaggtccg
     1741 ggtgatcagt tcgtcgagct ttaggcggcc gacgctgtag aggccgagca ggcgcggaaa
     1801 gtcctcgcgc ggccgcgcgg agccgaagta gctgccggtc agcgtcttct cgccgaaggt
     1861 gaggctggcg gtgcgtatcg tggttaagtc cttcggggcg gccacgccga ccaccactgc
     1921 agtgccgccc ttgcgcagca cgccgtaggc ctgggccgcg atctcgccgt gccccacgca
     1981 ttcgaatgca taatccgcgc cgccgtcggt cagcttcagg atgcgtttga ccacgttctc
     2041 ttcggccgtg ccgttgacca catgcgtggc cccgaactcg cgcgccatgt ccagcttggc
     2101 gtcggaggtg tcgaccgcga cgatcatccg cgcgccggcg atcgcgcagc cctgtatcgc
     2161 attcagtccg acgccgccgc agccgaacac gaccgcgatc gagccggcat ccaccctggc
     2221 cgtattgacg gccgcgccga cgccggtcat caccccgcaa ccgatcagtg ctgccttgtc
     2281 gagcgccacg tcattgtcga tgcgcacgac gttgtcgaca tgcagggtcg cgtattcggc
     2341 catcacgccg cagccggaga agacgttgag ccggttgccg gacagatcgc gcgtacgtac
     2401 ggttccgtcc ggcagcgtgt acgccgcgcg cgcggcctgg tcgcacagtt gcggacggcc
     2461 ggtctggcag tagcggcagc ggccgcacat gctgacgaag gaactgagca cgtggtcgcc
     2521 gaccgacagg tcgaccacgc cctcgccgac cgcaaccacg cggcccgcgc cttcatgccc
     2581 gagaatgacc ggaggcggga aaggaatcgt gccgttggtc gccgacaggt cgctgtggca
     2641 gacgccgcag gcggccagct tgatcatcac ttcgccacgg cccggcgcct cgacgatgat
     2701 gtcctcgacc acgaccggct ggttcagctc gcggcagatc accgccttcg cgcgttgttc
     2761 catctgtctc gttcctgaaa gtgaagaaag ccggctgatg ccgggaatgt cgttcacttc
     2821 agcggctgcg ccatggcatg ggcagtcgtc aggcgcgcgg cgttccgtgc atcggccggc
     2881 tcgtcgggcc ccgcctgtgc ggggccttgg gagcaggtga aggtgcttac ttcgcgccca
     2941 gatcgcgcag gctcacctgg cgcaccttgc cgttctcgac ggtggcgaca taggtgttgg
     3001 ccagcgagcc gccgttcgca tcgacgccgt tgatgtcgtt cgggttgtgc tcagccggca
     3061 gttccttgaa tgcgcggtcc atctgggcgc ggatcttggc cggatcgtcc acggtgccgg
     3121 ccagctgcat cgccagcgcg gtcgcgtgca cggcggtgta gttcagcgac acctcggagc
     3181 tcgggtcgcg gcccggattc atcttgcgga agcgctcgac ccaagattgg gtctgctggc
     3241 gcgagtcctc gatcaccggc agcacgccaa tcgatccttc gatcggaccg tagccgccga
     3301 tcaccttggc catctcgtcc atcttggcct ggtcgatgat gatgaaaccg cccttgaagc
     3361 ccagctcgcg cgcctgcttg accaccagcg cggtcggttc ggacgcgccg ccgatgaaca
     3421 ggacatccgg cttggcctgc agtacgcgcg acacaccgga gtagaagtcg gtggccttgt
     3481 tgtaggacat cgggttttcc gccaccacct tgccgccggc ggcttcccag gccggcttga
     3541 aggctgcggt ccaggccttc gcgtagtcgt ggtcggcgtt ggcgatggcc acgttcttgc
     3601 cgaacttgct catcgcatgc ttggtgaagg gctcgatgta gccggtgaat tcggggggaa
     3661 tgcggatcgt gagcttgttg ccgcgcgcgg tcgtctgcgg caggctcgta taggccagca
     3721 gcagcacgtt ggagcgctcg ttgaacgcct gcaccgcgaa gatgccgccc gagtgcggga
     3781 ccagcaccgc cggcgtcttg tgctgctgga tcaggcgctg cacgttgatg ccggtttcgg
     3841 acgggttgta cttgtcatcc agcgacacga tctcgagctt gtatttcttg ccgccgacct
     3901 cgaggccctt tgcattgatt tcctgcaccg ccatttccat gccggacagc acgttcttgc
     3961 cgtacagtgc ggcgccgccg gacagcgggc cggaaaagcc gatcttcacg acttcctgcg
     4021 ccatcgcagc acctgcgaag gacagaccga cagccaaggc tgcggtaatt gcatgcttct
     4081 tcatcgcttg tctcctgtgg tgggttgtct actgc
//
LOCUS       DATLGF010000067         3707 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_129906, whole genome shotgun sequence.
ACCESSION   DATLGF010000067 DATLGF010000000
VERSION     DATLGF010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129906"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3525
                     /gene="hrpA"
                     /locus_tag="VM406_02075"
     CDS             <1..3525
                     /gene="hrpA"
                     /locus_tag="VM406_02075"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HVL74778.1"
                     /translation="VGYKVRFTDTLSKGASVKLMTDGILLAETQTDPLLRQYDTIIID
                     EAHERSLNIDFLLGYLKQLLPKRPDLKLIITSATIDADRFARHFARGDAPAPVIEVSG
                     RLYPVELRYRPVEPAQKEESKTDGRKDARREQRDLMDAVVDAVDELARIGPGDVLVFL
                     PGEREIRDAAEALRKHHPPHVEILPLFARLSAQEQERVFKPSNARRIVLATNVAETSL
                     TVPGIRYVVDTGLARVKRYSYRNKVEQLQIEPIAQSAANQRAGRCGRVANGICIRLYD
                     EADFNSRPRFSDPEILRSSLAAVILRMKSLRLSDVETFPFIEPPPGRAIADGYQLLQE
                     LGAVDDDNRLTPLGRQLARLPLDPRVGRMILAARDNNALAEVLVIAAALSVQDPRDRP
                     LDAQDAADNAHRKFADEKSEFLAYLRIWKWFEEAVEHKKSNKQLAENCRANFLSQLRL
                     REWRDVHSQLLTLVREQGWRLNQTPATYEQLHTALLTGLLGNVGYKLEDDPQYLGARG
                     IKFLIWPGSSLAKKAGRWIMAAELVDTTRLYARCVAQIQPEWLERIGAHLLKKSWGEP
                     RWEKRAAQVVASERATLYGLVVYSQRRMNYGAINPKEAREIFIRSALVQGEYETRAPF
                     FAHNQKLVREIENLEHKSRRLDVLVDEELIVAFYDKLVPRHIHDGVAFEKWLKEATAA
                     NPKLLHLNRDDLMRHEAAGVTTDLFPKAMTVAGVSMALTYHFEPGSPRDGVTLAIPLF
                     ALNQVSAERCEWLVPGMLKEKVHLLLKSLPQKLRRHCVPLPDYAAGFCERAAFGKGSL
                     IDALIADVREHAGVMPKTADFKVETLPAHLFMNFKVIDEHGRQLEMGRNLAGLQAQFG
                     GQARERFQQLAEKTIVATVTAEHVPTPNKAAAAPAQHQNLTGWSFGELPELLEIQQGK
                     QTLIGFPALVDKGSHCDIEVFDDPAEAARIHHAGLRRLFALNLKEQLKFLEKNVPSLQ
                     QMGMQFMSLGTQEELREQIVQAGMDRAFMLDPLPKNGDEFMRRRDEGKARLNLLVNEI
                     ARLAGQILAEYHALPKKLQGVKAHAQSAADIQSQLQALIGKRFISDTPYEQLVHFPRY
                     LKAITVRLDKLRADPARDQRLMGEWAQVALPWQRAQKDRQKNADPRMQEFRWLLEELR
                     VSLFAQELRTPMPVSVKRLQKVWESMQR"
BASE COUNT          707 a         1176 c         1221 g          603 t
ORIGIN      
        1 gtcggctaca aggtccgctt caccgacacg ctctccaagg gcgcatcggt caagctgatg
       61 accgacggca tcctgctcgc cgagacccag accgatccgc tgctgcggca gtacgacacg
      121 atcatcatcg acgaggcgca cgaacgcagc ctgaacatcg atttcctgct cggctacctg
      181 aagcagctgc tgccgaaacg tccggatctg aagctgatca tcacctcggc caccatcgac
      241 gcagaccgct tcgcgcgcca cttcgcccgg ggcgatgcgc ccgcgccggt gatcgaggtg
      301 tcggggcgcc tgtacccggt tgagctccgc taccgtccgg tggagccggc gcagaaggaa
      361 gagagcaaga cagacgggcg caaggatgcg cggcgcgagc agcgcgacct gatggacgcg
      421 gtcgtcgatg cggtcgacga gctggcgcgc atcggtcccg gcgatgtgct ggtcttcctg
      481 cccggcgaac gcgagatccg cgacgccgcc gaagcgctgc gcaagcatca tccgccgcac
      541 gtggaaatcc tgccgctgtt cgcgcgtctt tccgcccagg agcaggagag ggtgttcaag
      601 ccgtcgaatg cgcgccgcat cgtgctggcg accaacgtgg ccgagacctc gctgacggtg
      661 ccgggcattc gttacgtggt cgataccggg ctggcgcgcg tgaagcgcta cagctaccgc
      721 aacaaggtcg agcagttgca gatcgagccg atcgcgcaat ccgctgccaa ccagcgcgcc
      781 ggccgctgcg gccgcgtggc caacggcatc tgcatccggc tgtacgacga ggccgacttc
      841 aacagccgcc cgcgcttttc cgacccggag atcctgcgct cctcgctggc ggccgtgatc
      901 ctgcggatga agtcgctgcg cctgtcggat gtcgagacct ttccattcat cgagccgccg
      961 ccgggccgtg ccattgccga cggctaccag ctgctgcagg aactaggcgc ggtcgacgac
     1021 gacaatcgcc tgaccccgtt gggccggcaa ctcgcccgtc tgccgctgga tccgcgcgtg
     1081 ggccgcatga ttcttgcggc gcgcgacaac aatgcgctgg ccgaagtgct ggtgatcgcc
     1141 gccgcgctgt ccgtgcagga tccgcgcgac cgcccgctcg acgcgcagga cgcagcagac
     1201 aatgcgcacc gcaagttcgc cgacgagaag tccgaattcc tggcctacct gaggatctgg
     1261 aaatggttcg aggaagcggt cgagcacaag aagtcgaaca agcagctggc cgaaaactgc
     1321 cgcgcgaact tcctgtcgca gctgcgcctg cgcgaatggc gcgacgtgca ctcgcagctg
     1381 ctgacgctgg ttcgcgagca gggctggcgc ttgaaccaga cgccggcgac ctacgagcag
     1441 ctgcatacgg cacttctgac cggcctgctc ggcaacgtcg gctacaagct cgaggacgat
     1501 ccgcaatacc tgggcgcgcg cgggatcaag ttcctgatct ggcctggatc gagcctcgca
     1561 aagaaagcgg ggcgctggat catggcggcc gaactggtgg acacgacccg gctgtatgcg
     1621 cgctgtgtcg cgcagatcca gcccgagtgg ctggaacgca tcggcgccca cctgctgaag
     1681 aaatcctggg gcgagccgcg ctgggaaaaa cgcgccgcac aggtggtggc atcggaacgt
     1741 gcgacgctgt acggcctggt cgtctacagc cagcgccgca tgaactacgg cgcgatcaat
     1801 ccgaaggagg cacgcgagat cttcatccgc agcgcgctgg tgcagggcga atacgaaacc
     1861 cgggcgccgt tcttcgcaca caaccagaag ctggtgcgcg aaatcgagaa cctggagcac
     1921 aagtcgcgcc ggctcgacgt gctggtcgac gaggaactga tcgtcgcttt ctacgacaag
     1981 ctggtgccgc ggcacatcca tgacggcgtc gcattcgaga aatggctgaa ggaagcgacg
     2041 gccgccaacc cgaagctctt gcacctgaac cgcgacgacc tgatgcgtca cgaagcggcc
     2101 ggggtgacga ccgacctgtt tccgaaggcg atgacggtgg ccggcgtttc gatggcgctc
     2161 acctatcatt tcgagccggg cagtccgcgc gacggcgtga cgctggcgat tccgctgttt
     2221 gcactgaacc aggtgtctgc cgagcgttgc gagtggctgg tgccgggaat gctgaaggaa
     2281 aaggtgcacc tgctgctgaa atctctgccg cagaagctgc gccgtcattg cgtgccgctg
     2341 ccggattacg ccgccggctt ctgcgagcgc gccgccttcg gcaagggcag cctgatcgac
     2401 gcgttgattg cggacgtgcg cgagcacgcc ggggtcatgc cgaagaccgc ggatttcaag
     2461 gtggagacgt tacctgccca cctgttcatg aacttcaagg tcatcgacga gcacggccgg
     2521 cagctcgaaa tggggcgcaa cctcgccggc ctgcaggcac agttcggcgg acaggcccgc
     2581 gagcgcttcc agcagcttgc cgaaaagacg atagtcgcca cggtcactgc tgagcatgtg
     2641 cctaccccga acaaggctgc cgcagcgcct gcccagcatc agaacctgac cggctggtcg
     2701 ttcggcgagc ttccggagct gctcgaaatc cagcagggca agcagacgct gatcggcttc
     2761 cccgcgctgg tcgacaaggg cagtcactgc gacatcgaag tgttcgacga tccagccgaa
     2821 gcggcgcgca tccaccacgc cggcctgcgc cgcctgttcg cgctcaatct gaaggaacag
     2881 ttgaagttcc tggagaagaa cgtgcctagc ttgcagcaga tggggatgca attcatgtcg
     2941 ctcggaacgc aggaagagtt gcgcgagcaa atcgttcagg ccggcatgga ccgagcgttc
     3001 atgctggatc cgctgccgaa gaatggggac gagttcatgc gccgccgcga cgaaggcaag
     3061 gcgcgcttga atctgctggt caacgagatt gcgcggctgg ccggacagat cctggccgag
     3121 tatcacgcgc tgccgaagaa gctgcagggc gtgaaggcgc atgcgcaaag cgcggctgac
     3181 atccagtcgc agttgcaggc attgatcggc aagcgcttca tctccgacac gccgtatgaa
     3241 cagctcgttc acttcccgcg ttacctgaag gcgatcactg tccggctcga caagctgcgc
     3301 gccgatccgg cgcgcgacca gcggctgatg ggcgaatggg ctcaggtggc gctgccctgg
     3361 cagcgcgcgc aaaaggacag gcagaagaac gccgatccgc gcatgcagga gtttcgctgg
     3421 ctgctggagg agttgcgcgt gtcgctgttc gcgcaggagt tgcgcacgcc gatgccggtg
     3481 tcggtgaagc ggctgcagaa ggtgtgggag tcgatgcagc gctagcgcgc gccagggcag
     3541 ttcaggtgct gtcccacgca cgaagggatg aggtccctcg ctatgttcgg gacaccagca
     3601 gattggggct gtttgccttg atctccatgt gcgccgtggt gacgaacatt ctccaaaaat
     3661 ttcgaaccac ggcgatcacg gcgcgcactg cgaaaaacct gtcattc
//
LOCUS       DATLGF010000068         5677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_35014,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000068 DATLGF010000000
VERSION     DATLGF010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5677
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35014"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3124
                     /locus_tag="VM406_02080"
     CDS             <1..3124
                     /locus_tag="VM406_02080"
                     /inference="COORDINATES: protein motif:HMM:NF017388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HVL74779.1"
                     /translation="YGKHYKPVGELQRNAGRVRVGAMGYLLDDSATRYGGVLRAPIKY
                     LGPHRHAAPGFVRMANERLEWNPETGVFYRNPEDPTARDSASVNSGVINYLNKFGRNS
                     QYKALDPVGELYYEGLRYLQGLQPTPEAVSDINDSMRDGFPVHASWNDPLLASCQRNY
                     ILAIADVNTHWDRYIPGNTRTTFGKNQNANDPARPLEPAVPGKTPALDVRTWTRKVGE
                     LEADAGGTFGNPAPRAHLAGLDTLDTGSGGHGTYYMAGLAYWANTQDIRVDKPARVQT
                     FAIDVDEGGNGIVDGNTRGLKPRDSQLYLAAKYGGFRDRNGDGNPFKTIGPDGKPVHD
                     PYGEWDNGEGVPANYFLAGQPREMIDAIRSVFAGISASSGTLSGVSLSTGRIGSDGLY
                     IYQPGFDPLRWSGTLRKLKLSLDDDGGVRVSDAAEWEAGAVLTGTANKASVPDANQRR
                     IFAGSGSGAGFKTVPFLWDSLDTAQRQALNRSSSDGAEDGLGAARVAYLRGTRNQELG
                     RPNGMFRQRDSLLGDIVNSNPVHVGPPSGNAHGPGYAEFREKYKSRVPAVYIGANDGM
                     MHAFSAADGRELFAYVPAALVPQLAQLTDPAYAHRPFVDGGLSVAEARVGDEWKTVLL
                     GAMGGGAQGVFALDVSDPGNFAAGAGALFEFTDADDPDIGNVMGAPAIARLRVGSKDG
                     VTQYKYFAIVASGLNNYVPDGEGRHNSDAAGALFLLALDKPAGVAWKAGVNYYKVRLP
                     AADKTKPNGLSQPALVAGADAAVHTVYVGDLQGQMWRIRLGTNPATLAGPGAATPIFT
                     AAHEGKIQPVTAQPRVAFAPGGYVVLFGTGKFIEAADAAPGGYEVQSFYAVLDTLAPG
                     YHASRSDLVARSLTPSADGGYQVTGDPVTYGNAAGSRKGWYIDFPDSDKSGERIVTNP
                     VVLNGYVFFNSLIPGSDPCAAGGGRSYAVGTLSGMPAQGRTIGHLSQVGMLSAPVVLE
                     SAVKRGARDAIGGRIVERRHQVVNFGTGGARGMGAPADNGSGAVTLRAGRLSWREVLN
                     WQELSNAGKK"
     gene            3108..3569
                     /locus_tag="VM406_02085"
     CDS             3108..3569
                     /locus_tag="VM406_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HVL74780.1"
                     /translation="MRERSSNRSSRGGFTLIELLVAVAVVAVLAAVAYPAYTESVRKA
                     RRAEARAALLQLMQQQERFYTQRNTYIAFSATSTDPDAKRFRWHSGDTPGTSAYQIRG
                     VACGTEPLTACVRLVAQAGGSHVDPAYKDSACPAFALLSNGQREGNAACWN"
     gene            3573..4061
                     /locus_tag="VM406_02090"
     CDS             3573..4061
                     /locus_tag="VM406_02090"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HVL74781.1"
                     /translation="MLQRGFSLIELMTVVAVAVIVLGIGVPGMRGLVVSQQLTGAANA
                     LHASIKLARSEAISRGARVEIAPAAPGVWAAGWVVYIDQDGNGSYGAGDQLLYSHGPL
                     RAGFMVTTTFAQNSIPYTGTGRALAAGTLKLSAHGQGRNITVNLLGRTRICRPAEVGK
                     DC"
     gene            4188..5258
                     /gene="ribD"
                     /locus_tag="VM406_02095"
     CDS             4188..5258
                     /gene="ribD"
                     /locus_tag="VM406_02095"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HVL74782.1"
                     /translation="MSLALDWAARAMFITTPNPRVGCVIVRDGQVLGAGHTLPPGQAH
                     AEVQALRDAAERGHHVRGATAYVTLEPCSHFGRTPPCADALVQSGVARVVAAVADPNP
                     LVAGQGLARLREAGIEVACGVLEDQAREMNIGFFSRMQRGRPWVRMKAAASLDGKTAL
                     HDGRSQWITSAAARDDGHWWRARACAVLTGIGTVMQDDPQMTVRAVQTPRQPRRIVID
                     SRLQIDPAARILEGGGTWIATAASNPDKEARLREQGAEILRLPDAQGKVDLAALIQEL
                     GRRQINEVHVEAGAKLNGSLIRAECVDELLLYFAPSLIGEARGMFVLPALEDLAAQRR
                     LAFHETTQIGPDLRILARFIRD"
     gene            5264..>5677
                     /locus_tag="VM406_02100"
     CDS             5264..>5677
                     /locus_tag="VM406_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006755087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HVL74783.1"
                     /translation="MFTGIVAAVGKILATTPLGSEPDAGLRLTVEANCQLMEDVALGD
                     SIAINGACMTVVEKTADQFTVDVSRESLNRTVGLDQPGEVNLEKALTLAERLGGHLVS
                     GHVDGLGTVRRFEPVGESMELVIEAPKELGKYLAYK"
BASE COUNT          981 a         1887 c         1917 g          892 t
ORIGIN      
        1 gtacggcaag cactacaagc cagtcggcga actgcagcgc aatgcggggc gcgtgcgcgt
       61 cggagcgatg ggctatctgc tcgacgactc ggcgacgcgc tatggcggtg tgctgcgggc
      121 accgatcaag tacctgggcc cgcatcgtca tgccgcgcct ggcttcgtgc gcatggccaa
      181 tgagcgtctg gaatggaatc cggagaccgg cgtgttctac cgcaatccgg aagacccgac
      241 tgcacgcgac agcgcgagcg tcaacagcgg ggtgatcaac tacctgaaca agttcggacg
      301 caacagccag tacaaggcgc tcgacccggt cggcgagctc tattacgaag ggttgcgcta
      361 tctgcagggc ctgcagccga ccccggaggc ggtctccgac atcaatgact ccatgcgcga
      421 cgggtttccg gtgcatgcga gctggaacga tccgctgctg gcgtcctgcc agcgcaacta
      481 catcctcgcg attgccgacg tcaacaccca ttgggaccgc tatatcccgg ggaacacgcg
      541 caccaccttt ggcaagaacc agaatgcgaa cgatccggcc cgtccgctgg agcccgcggt
      601 gcccggcaag actcctgcgc tcgacgtgcg cacctggacg cgcaaagtcg gtgaactgga
      661 ggccgacgcc ggtggcacat tcggcaatcc ggctccgcgt gcgcacttgg cagggctcga
      721 taccctggat accggctcag gcggtcacgg cacctattac atggcggggc ttgcgtactg
      781 ggcgaacacc caggacatcc gcgtcgacaa gccggcacgg gtgcagactt tcgcgatcga
      841 cgtcgatgaa ggcggaaacg gcattgtcga cggcaatacc cgcggcctca agccgcgcga
      901 cagccagctc tacctcgcgg ccaagtacgg cggtttccgc gaccgcaacg gcgacggcaa
      961 tccattcaag accatcggcc cggacggcaa gccggtgcac gacccgtatg gcgaatggga
     1021 caacggcgaa ggcgtgccgg caaattactt tctcgctggc cagccgcgcg agatgatcga
     1081 cgcgatacgc agcgtgttcg ccggcatctc ggcaagcagc ggcacgctgt ccggggtgtc
     1141 gctgtcgacc ggccgcatcg gcagcgacgg cctgtacatc tatcagcccg gcttcgatcc
     1201 gctgcgctgg agcgggacgc tgcgcaagct gaagctcagc ctggacgatg acggtggcgt
     1261 gcgggtttcc gacgccgccg aatgggaggc cggcgcggtg ttgacgggca cggcgaacaa
     1321 ggcgtcggtg ccggatgcga accagcgccg gatctttgcc ggcagcggca gcggagccgg
     1381 cttcaagacc gttccctttc tctgggacag cctggacacg gcgcagcggc aggcgctaaa
     1441 ccgctcttca tccgacggcg ccgaggatgg actcggcgcg gcgcgcgttg cctacctgcg
     1501 cggaacacgg aaccaggagc tggggcggcc aaacggcatg tttcgccagc gtgacagcct
     1561 gcttggtgac atcgtcaaca gcaatccggt gcatgtcggc ccgccttcgg gcaatgcgca
     1621 cggccccggc tatgccgaat tccgcgagaa gtacaagtca cgtgtgcccg cggtctacat
     1681 cggtgccaat gacggcatga tgcacgcgtt ctccgcggcg gacgggcgcg aactgtttgc
     1741 gtatgtcccc gccgcgctcg ttccccagct tgcgcaattg accgaccccg cctatgcgca
     1801 ccggccgttc gtggacggcg gcctgtcggt cgccgaagcg cgcgtcgggg acgagtggaa
     1861 gacggtgctg cttggcgcaa tgggtggcgg tgcgcagggc gtgttcgcgc tggacgtgag
     1921 cgatccgggg aatttcgccg ccggtgcagg cgcgctgttc gagttcacgg acgccgacga
     1981 tcccgacatc ggcaatgtga tgggcgcccc cgccatcgcc cgcctgcggg ttggcagcaa
     2041 ggacggcgtg acccaataca agtacttcgc gatcgtcgcc agcggcctga acaactacgt
     2101 gccggacggc gaggggcgcc ataacagcga tgcggcaggc gcgctgttcc tgctcgcgct
     2161 ggacaaaccg gccggcgttg cctggaaggc gggcgtcaat tactacaagg tccggctgcc
     2221 ggccgcggac aagacgaagc cgaacggctt gtcgcaaccg gcgctggttg ccggcgcgga
     2281 cgccgcggtg cacaccgtgt acgtcggcga tctgcaaggc cagatgtggc gaatccggct
     2341 cggcacgaat ccggctacgc ttgcgggccc aggcgccgcc acgcccatct tcacagctgc
     2401 gcacgaaggc aagatccagc cggtcaccgc gcagccgcgc gtggcgttcg ccccgggcgg
     2461 ctatgtggtc ctgttcggca ccggcaaatt catcgaagcc gccgacgccg cgcctggcgg
     2521 ctacgaggtc cagtcctttt acgcggtgct cgataccctt gcaccgggct accacgcaag
     2581 ccgtagcgac ctcgtcgcgc gcagcctcac gccgagtgcg gacggcggct atcaggttac
     2641 cggtgatccc gtcacctacg ggaacgcggc gggcagccgc aagggctggt acatcgattt
     2701 ccccgattcg gacaagagcg gcgagcgcat cgtgacgaac cctgtcgtgc tcaatggcta
     2761 cgtgttcttc aacagcctca tcccaggcag cgacccctgc gccgccggcg gcggtcgctc
     2821 gtatgcggtc ggcacgctct ccggcatgcc ggcccagggc cgcacgatcg gccacctgtc
     2881 gcaggtgggc atgctcagcg cgccggtcgt gctggaaagc gcggtcaagc gcggcgcgcg
     2941 cgatgcgatc ggcggacgga tcgtcgagcg ccggcatcag gtggtcaatt tcggcaccgg
     3001 cggcgcaagg ggcatgggcg cgccggcgga caacggcagc ggtgcggtga cgctgcgggc
     3061 agggcgcctg agctggcgcg aagtgttgaa ctggcaggag ttgagcaatg cgggaaagaa
     3121 gtagcaacag gagcagccgc ggcgggttca ccctcatcga actgctggtc gccgtggcgg
     3181 tcgtcgcggt tctcgccgcc gtcgcctatc cggcctatac cgagtcggtg cgcaaggcca
     3241 ggcgcgcgga ggcgcgcgcc gcgctgctgc aactgatgca gcagcaggaa cgcttctata
     3301 cccagcgcaa cacctatatc gcgttctccg ccacttccac cgatccggat gcgaagcgct
     3361 tccgatggca ctccggcgat acgccgggga cgagcgccta ccagatccgc ggcgtcgcct
     3421 gcgggaccga accgctcacc gcctgcgtgc ggctcgtggc gcaggccggc ggcagtcatg
     3481 tcgacccggc ctacaaggac agcgcctgtc ccgcctttgc cttgctgagc aacggccagc
     3541 gggaaggcaa tgccgcctgc tggaactagc tgatgctgca acgcggattc tcgctgatcg
     3601 agctgatgac cgtcgtcgcg gtcgccgtca tcgtcctcgg catcggcgtg ccgggcatgc
     3661 gcgggctcgt ggtcagccag caattgaccg gcgctgccaa cgccctgcat gcgtccatca
     3721 agctggcccg ctccgaagcg ataagccgcg gcgcacgcgt ggaaattgcg cctgccgcgc
     3781 caggcgtctg ggccgccggc tgggtggtgt acattgatca ggacggcaac gggagttacg
     3841 gggcgggcga ccagctgctt tactcgcacg gccccttgcg agccggattc atggttacga
     3901 ctacgtttgc gcagaacagc attccctaca ccggcaccgg ccgcgcgctt gccgcgggta
     3961 ccctgaagct gtcggcgcat gggcagggca ggaacatcac cgtcaacctg ctcggccgca
     4021 cacgtatctg ccggccggcc gaggtgggca aggattgctg acgcggcgac cctttccaga
     4081 tctgtaacgg cgcggccttg cggctacaat cacgcctatc cgccgcaacg gccccaggat
     4141 ccgccttgac tgcagcttcc gctcccatcc tcgacgacgc ccaggccatg tcgctcgccc
     4201 tcgactgggc ggccagggcc atgttcatca ccacgccgaa tccgcgcgtc ggctgcgtga
     4261 tcgtgcgcga cggccaggtg ctgggggcgg gacacacgct gccgcccgga caggcgcacg
     4321 cggaagtgca ggcgctgcgg gatgcggccg agcggggcca tcatgtgcgc ggcgcaaccg
     4381 cctatgtgac gctggagccg tgcagccatt tcggacggac gcccccttgc gccgacgcgc
     4441 tcgtccaatc cggcgtcgca cgtgtggtcg cggccgtggc ggacccgaac ccgctggttg
     4501 cgggacaagg tcttgcgcgg ctgcgcgaag ccggcatcga agtcgcctgc ggcgtgctgg
     4561 aagaccaggc acgcgagatg aatatcggat tcttctcgcg catgcagcgc ggccgtccct
     4621 gggtacgcat gaaagccgcc gccagtcttg acggcaagac cgccttgcat gacggccgca
     4681 gccaatggat tacgtccgcc gccgcccgcg acgacggcca ctggtggcgc gcccgagcat
     4741 gcgcggtgct aaccggcatc ggcaccgtca tgcaggacga tccgcagatg acggtgcggg
     4801 ccgttcagac gccgcgccag ccgcgccgca tcgtgatcga cagccggctc cagatcgacc
     4861 cggccgcacg catactggaa ggcggcggca cctggatcgc aaccgctgcg tccaacccgg
     4921 acaaggaggc gcggctgcgc gagcaggggg ccgagatcct ccgcctcccg gatgcgcagg
     4981 gcaaggtcga cctcgccgcg ctgattcagg aactcggtcg gcgccagatc aatgaagtgc
     5041 atgtcgaagc cggcgcgaaa ctcaatggtt cgctgatccg cgcagagtgt gtcgatgagt
     5101 tgctcctgta tttcgcgccc agcctgatcg gcgaggcgcg cggcatgttt gtgctgcccg
     5161 cgctcgaaga ccttgcggcc cagcgccggc ttgccttcca cgaaaccacg cagatcggtc
     5221 ccgatttgcg tatccttgcc cgttttatca gagattgatc gccatgttta ccggaatcgt
     5281 tgccgccgtc ggaaaaatcc ttgccaccac gccgctcgga agcgaaccgg acgcgggcct
     5341 gcgcctgacc gtcgaagcca attgccagtt aatggaggat gtcgcgctcg gagactccat
     5401 tgcaatcaac ggcgcctgca tgacagtggt cgagaagacc gccgatcagt tcactgttga
     5461 tgtgtcgcgc gaaagcctga accgcacggt cggactggac cagcccggcg aggtcaacct
     5521 ggaaaaggcg ctcacactgg ccgagcgcct cggcggacac ctggtatccg gccacgtgga
     5581 cgggttgggc acggtgcgcc gtttcgagcc ggtcggcgag tcgatggagc tggtgatcga
     5641 ggcgccgaag gaattgggga aatacctggc ctacaag
//
LOCUS       DATLGF010000069         2163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_223401, whole genome shotgun sequence.
ACCESSION   DATLGF010000069 DATLGF010000000
VERSION     DATLGF010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223401"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..909)
                     /locus_tag="VM406_02105"
     CDS             complement(148..909)
                     /locus_tag="VM406_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL74784.1"
                     /translation="MNLHGIKAIYHFEMARWWRTLLQSIASPVISTSLYFIVFGAAIG
                     ARMEQIDGISYGAYIVPGLIMLSVLTESISNASFGIYMPKWAGTIYEVLSAPVSPLEI
                     VIGYVGAAATKSILLGLIILVTARLFVDYSILHPLWMIGFLVLTSVTFSLFGFVIGIW
                     ADGWEKLQIVPVMIVTPLAFLGGSFYSIRMLPDFWQQVTLFNPVVYLVSGFRWSFYGV
                     SDVDVTISLGMTLAFMVACLGLIWWIFRTGYRLKK"
     gene            complement(906..1841)
                     /locus_tag="VM406_02110"
     CDS             complement(906..1841)
                     /locus_tag="VM406_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL74785.1"
                     /translation="MQPVISIQALTKTYASGFQALKEIHLDIRRGEIFALLGPNGAGK
                     TTLISIICGIVTASTGTVLADGHDIARDYRAARSKIGLVPQELSTDAFERVIDTVRFS
                     RGLFGKAPDDAYLEKVLRDLSLWDKRNEKIMALSGGMKRRVMIAKALSHEPNILFLDE
                     PTAGVDVELRRDMWEMVRRLRESGVTIILTTHYIEEAEEMADRIGVINKGEILVVEDK
                     DVLMKKLGKRQLTLLLQQPVESVPQELARFGLELSSDGSQLVYTFDAMGEHPAVPVLL
                     QQLAAHGIHFKDLQTRESSLEEIFVNLVRGERSRT"
BASE COUNT          396 a          676 c          702 g          389 t
ORIGIN      
        1 gggctacgcg ctcggcaggc cggagccgat gacgagcgtc cctcgtgtgt ccgaaggcga
       61 ggtgcggcca ggcgcggcga cgctttgggc gagcgcgcgc tgacgcttcc gtgtcgggtg
      121 cctggcgtcg cgcaggcgcc gggcgtgcta cttcttgagc ctgtacccgg tccggaaaat
      181 ccaccagatc aagccgaggc aggcgaccat gaacgcgagc gtcatgccca ggctgatcgt
      241 cacatccaca tccgacacgc cataaaagct ccagcggaag ccgctgacca ggtatacgac
      301 cggattgaac agcgtgacct gctgccagaa gtccggcagc atgcggatcg aatagaaact
      361 tccgccgaga aaggccagcg gcgtgacgat catcaccggt acgatctgca gcttctccca
      421 cccgtcggcc cagatgccga tcacgaagcc gaacaggctg aaggtcaccg aagtcaggac
      481 caggaagccg atcatccaga gtgggtgcag gatcgaatag tcgacgaaca ggcgcgcggt
      541 gacgaggatg atcaggccga gcaggatgga cttggttgcg gccgccccca catagccgat
      601 cacgatctcc agcggcgaga caggtgcgga cagcacctcg tagatggtcc ccgcccactt
      661 cggcatgtag atgccgaacg aggcgttgga aatgctttca gtcagcaccg acagcatgat
      721 cagacccgga acgatgtatg ccccgtagct gatgccgtcg atctgctcca tgcgcgcgcc
      781 gatggccgcg ccaaacacga tgaagtacag cgaggtcgag atcaccggcg aggcgatgct
      841 ttgcagcagc gtgcgccacc agcgcgccat ttcgaagtga tagatcgcct tgattccatg
      901 caggttcatg tccggctccg ttcgccgcgc accaggttga cgaagatttc ctccagcgag
      961 ctctcgcgcg tctgcaggtc cttgaagtga atgccgtgtg cggcgagctg ctgcagcagc
     1021 accggcacgg cagggtgctc gcccattgca tcgaaggtgt agacgagctg gctgccgtct
     1081 gacgaaagct ccaggccgaa gcgtgcaagt tcctgcggca cggactccac cggttgctgc
     1141 agcagcaggg tcagctggcg cttgccgagc tttttcatca gcacgtcctt gtcctcgacc
     1201 accaggatct cgcccttgtt gatcacgccg atgcggtcgg ccatttcttc cgcctcttcg
     1261 atgtagtggg tggtcaggat gatggtcacg ccgctttcgc gcaggcgcct gaccatctcc
     1321 cacatgtcgc gccgcagttc gacgtcgacg ccggcggtcg gctcgtcgag gaacaggatg
     1381 ttcggttcat ggctcagcgc cttggcgatc atcacgcggc gcttcatccc gcccgacagc
     1441 gccatgatct tctcattgcg cttgtcccac agcgacaggt cgcgcagcac cttttcgaga
     1501 taggcgtcgt cgggcgcctt gccgaacagg ccgcgggaaa agcgcacggt gtcgatcacg
     1561 cgttcgaagg cgtcggttga aagttcctgc ggcaccaggc cgatcttgct gcgcgcggcg
     1621 cggtagtcgc gcgcaatgtc gtggccgtcg gccaggacag tcccggtgct ggcggtgaca
     1681 atgccgcaga tgatgctgat gagggtggtt ttcccggccc cgttgggtcc gagcagcgcg
     1741 aaaatttccc cgcgccggat gtcgagatgg atttccttga gggcctggaa gccggacgcg
     1801 taggtcttgg tcagggcctg tatcgaaatg actggctgca catgcgctcc gtcgagacgt
     1861 gagggcggcc ggcctgagcc cggctcctgc ctcgccccct gttactgcct gcaaaggggc
     1921 acatgttaaa acatcaagcc gtttgctgcg cgacgctctc ggccgtgtgc acgcggttgc
     1981 gtcccgcgcg cttggcccgg taaagggcct ggtcggcggc cgcgagaagc tggtccatgc
     2041 cgccaccggt tccgggtcaa gcgcgcggga cgcaaccgcg tgcacacggc cgagagcgtc
     2101 gcgcagcaaa cggcttgatg ttttaacatg tgcccctttg caggcagtaa cagggggcga
     2161 ggc
//
LOCUS       DATLGF010000070         3122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_82343,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000070 DATLGF010000000
VERSION     DATLGF010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82343"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VM406_02115"
     CDS             complement(<1..228)
                     /locus_tag="VM406_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HVL74786.1"
                     /translation="MVATTRRATPGTQRGVSLVEALVTIVIIALGLLGLVGLQSRLQL
                     SEIEAYQRSQAMILLEDMANRISMNRAAIANY"
     gene            complement(222..635)
                     /locus_tag="VM406_02120"
     CDS             complement(222..635)
                     /locus_tag="VM406_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HVL74787.1"
                     /translation="MASPAIVTKIARGFTLIELMIAVAVIAILASIALPSYQQYVVRS
                     HRTAAQAQMMEIANLQQQYFLANRAYADNDDLEDMGYSLPAEVASRYTYASTPGTGAV
                     PSFTITFTPIAGGPQAADGALTLNSDGRKMPANKW"
     gene            781..1770
                     /locus_tag="VM406_02125"
     CDS             781..1770
                     /locus_tag="VM406_02125"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HVL74788.1"
                     /translation="MDKSDLDSYATLSPETILDAVEGFGLRPDGRLLALNSYENRVYQ
                     LGLEEGRPVVAKFYRPGRWSDHQIEDELDFVDELAEAEIPVVPAMTGPDGSTLLHFAG
                     FRFALFPLRGGRPPELEDRHTLEWIGRFLGRIHAVGARRSFVHRPPLDASTFGDEPLA
                     YLLENDWLPAELRAAYESVARQALEAVRACYARAGEVRTIRLHGDCHAGNVLWTDAGP
                     HFVDFDDSRMGPAVQDLWMLLSGERGDMTRQLADVLAGYEDFRNFDPRELHLVEALRT
                     LRLIHYSAWIARRWEDPAFPVAFPWFNTARYWQDRILELREQVALMQEPPLAV"
     gene            complement(1797..2135)
                     /locus_tag="VM406_02130"
     CDS             complement(1797..2135)
                     /locus_tag="VM406_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74789.1"
                     /translation="MHTIPETKKSHLWRKVVWHTDPEQYPLGPYHSVEVYCCEEANGY
                     SVWYVRRLGKDDRRGLAGMENGDYLLNYFPKTRRDDAIERAVLIANGDSVVEKTIEQL
                     DALAKAAQRV"
     gene            complement(2162..2596)
                     /locus_tag="VM406_02135"
     CDS             complement(2162..2596)
                     /locus_tag="VM406_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HVL74790.1"
                     /translation="MDETDNKILALLMDDARMATTTIAKRVGIARTTAIARIAALEKR
                     GVIAGYGVRLNQALYQPAVRAYVGVAVDPRKAAGLVKALEKMPQLETLCAVSGPVDYM
                     LTLRCQSTAELDGLLDRIGAVDGVRQTSTSIILTKRIDRSPL"
     gene            2639..>3122
                     /locus_tag="VM406_02140"
     CDS             2639..>3122
                     /locus_tag="VM406_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HVL74791.1"
                     /translation="MESDVPTTPCQLFSDNTWAHTFLIRSSTKTKGSAMKSRLILLGA
                     GKIGDAILNLLSHSGDYRITVADRDPARLAHIDALRFPDVQTVRADVSEAGALVDLID
                     GHEVTVSASPYFLTPAIAAAAKKAGSHYFDLTEDVESTRAVKQLAEDTDTAFVPQCGL
                     A"
BASE COUNT          505 a          991 c          994 g          632 t
ORIGIN      
        1 gtagttggca atggcggctc ggttcattga aatgcggttc gccatatcct ccagcaggat
       61 catcgcctgg ctgcgctggt aagcctcgat ctccgacagt tgcaatctgg actgcaggcc
      121 caccaatccg agcaggccca atgcgatgat gacgatggtg accagcgcct cgaccaggga
      181 gacgcctcgc tgtgttccgg gagtggcccg ccgagtggtc gctaccattt gttagcaggc
      241 attttgcgcc cgtcgctgtt aagggtgagg gcgccatcgg cggcttgggg gccgcccgca
      301 attggcgtaa aggtgatcgt gaagctcggg accgcccctg ttcccggtgt gctcgcatac
      361 gtgtagcgac tcgcgacttc tgccgggagc gaatagccca tgtcctccag gtcgtcgttg
      421 tcggcgtagg ccctgttggc gaggaagtac tgctgctgca ggttcgcaat ctccatcatc
      481 tgcgcctgcg ctgccgtccg atggctgcgt acgacatact gctggtagct cggaagtgcg
      541 atcgatgcca ggatggcaat taccgccacg gcgatcatga gttcaatcag cgtgaaccct
      601 cgggcgatct tggtgacaat tgcaggagat gccatgcggt cctctggagg agagtttctc
      661 gtcggcaata ataataatag gaaatttccg cgcggcagcg attttgttgt gacgagcggt
      721 cggtttctgg tgacaaacgg caaccatggt gttgtgaatc cgtcctttct tgctccccac
      781 atggacaaat cagaccttga tagttatgca acgctctcgc ccgagaccat ccttgatgcg
      841 gtcgagggct ttgggcttcg tccggacggc cgtctgctcg cgctcaacag ctatgagaac
      901 cgggtttatc agcttggcct cgaggagggg cgcccggtgg tcgccaagtt ctaccggccg
      961 ggcaggtggt ccgatcacca gatcgaggat gagctcgact ttgtggatga actggccgag
     1021 gcggagatcc ctgtcgtgcc agcaatgacg gggccggacg gcagcacgct tctgcacttc
     1081 gccggctttc gatttgcgct gtttccgctt cggggcggac gcccgcccga gcttgaagac
     1141 cgccatacac tcgagtggat cggccgcttc ctcggccgca tccatgcggt cggcgcgcga
     1201 cgctcgttcg tccaccgtcc gcccctcgat gcatcgacgt tcggcgacga accgctggcc
     1261 tatctgcttg agaacgactg gctgccggcc gagctgcgcg ccgcttacga aagcgtggcc
     1321 cgccaagcgc tggaggcggt gcgcgcctgt tatgcccgcg ccggcgaggt gcgcacgatc
     1381 cgcctgcatg gcgactgcca cgccggcaac gtgctgtgga ccgacgccgg cccgcatttc
     1441 gtcgatttcg acgacagccg catgggaccg gcggtgcagg acctgtggat gctgctgtcc
     1501 ggcgagcgtg gcgacatgac ccgccagctg gccgacgtgc tggccgggta tgaggatttc
     1561 cgcaatttcg atccgcgcga gctgcatctg gtcgaggcgc tgcgtaccct gcgcctgatc
     1621 cactactcgg cctggattgc gcggcgctgg gaggacccgg ccttcccggt cgccttcccg
     1681 tggttcaaca ccgcgcgcta ctggcaggac cgtatcctcg agctgcgcga gcaggtggcg
     1741 ctgatgcagg agccgccgct cgcagtgtga caggaccgat ccctgccgga acaggcttac
     1801 acccgctgcg cggccttcgc gagcgcgtcg agctgctcga tggtcttttc gaccacgctg
     1861 tcgccgttgg cgatcagcac cgcgcgctcg atcgcgtcgt cgcggcgcgt cttcgggaag
     1921 tagttgagca ggtagtcgcc gttttccatc cccgccagcc cgcgccggtc atccttgccg
     1981 agcctgcgca cgtaccagac cgaatagccg ttcgcttcct cgcagcagta aacctcgacc
     2041 gagtggtacg ggcccaatgg gtactgttcc gggtcggtat gccagacaac cttgcgccac
     2101 aggtggctct tcttcgtttc ggggatggtg tgcatgtgga gtcctggtcg ttgtgccgcg
     2161 gtcagagcgg gcttcggtct attctcttgg tcaagataat cgaggtcgag gtttgccgca
     2221 cgccgtcgac tgcaccgatc cggtcgagca ggccgtccag ttcggcagtg gactggcagc
     2281 gcagcgtcaa catgtagtcg accggtccgc tgaccgcgca cagcgtctcc agctgcggca
     2341 tcttctccag cgccttgacc agcccggccg ccttgcgcgg atcgaccgcg acgccgacgt
     2401 aggcgcgcac ggccggctga tacagcgcct ggttcagccg cacgccgtag ccggcgatca
     2461 cgccgcgctt ttccagtgcg gcgatgcgcg cgatcgcggt cgtgcgcgcg atgccgacgc
     2521 gcttggcgat cgtcgtggtg gccatgcgcg cgtcgtccat cagcagcgca agaatcttgt
     2581 tgtcggtctc gtccatccgg gtttgctgcg ctttgtttcg ttcgattgac gaattgtagt
     2641 ggaaagcgac gttccgacga caccgtgcca gctgttttct gacaacacct gggcgcacac
     2701 tttccttatt cgctcatcaa caaaaacgaa aggaagtgcg atgaaatccc gcctgattct
     2761 cctcggcgcc ggcaaaattg gcgacgccat cctgaacctg ctctcgcaca gcggcgacta
     2821 ccggatcacg gtggcagatc gcgatccggc gcgccttgcg catatcgacg cgctgcgctt
     2881 tccggacgtg caaaccgtgc gggccgacgt ctcggaggcg ggcgcgctgg tcgatctgat
     2941 cgacggccac gaagtcacgg tgtcggcctc gccctatttc ctcaccccgg caatcgccgc
     3001 cgcggcgaag aaggcgggtt cgcattactt cgacctgacc gaggacgtcg agagcacgcg
     3061 cgccgtcaag caactggccg aggataccga caccgccttc gtgccgcagt gcggtcttgc
     3121 ac
//
LOCUS       DATLGF010000071         2107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_19480,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000071 DATLGF010000000
VERSION     DATLGF010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19480"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VM406_02145"
     CDS             complement(<1..331)
                     /locus_tag="VM406_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HVL74792.1"
                     /translation="MKSGKFRELVLGKALDPLKPETRHSLALVAFFAWVGLGADGLSS
                     SAYGPEYTFRALGENTDLGLYLAFATAATVFIIALAYNQVIELFPTGGGGYRVATKLV
                     GPYMGLIA"
     gene            501..585
                     /locus_tag="VM406_02150"
     tRNA            501..585
                     /locus_tag="VM406_02150"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Leu,seq:caa)
     gene            721..1629
                     /locus_tag="VM406_02155"
     CDS             721..1629
                     /locus_tag="VM406_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HVL74793.1"
                     /translation="MPVSLDNLLVVGISSRALFDLEAEEAIFRTQGLEAYRRHQVEHE
                     NEILKLGGGFALVRALLKLNQLAHNQRFVEVVIMSRNSTETSLRIFNSIKHYGLDITR
                     AVLSGGAPLAPYLRAFNVSLFLSLHEDDVQLAIDSGVAAALLYQKPDNAFEELDQIRI
                     AFDGDAVIFSDESERIFQTQGIEAFEHHERENALRPLPEGPFARLLKALSFIQNNLKT
                     ADGGKAPIRTALVTARSSPAHERVIRTLRAWDVAIDETFFMGGVAKQDVLAAFKPHIF
                     FDDQQGHCERAAPLVQTGRVPVKSIG"
     gene            complement(1712..>2107)
                     /locus_tag="VM406_02160"
     CDS             complement(1712..>2107)
                     /locus_tag="VM406_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HVL74794.1"
                     /translation="DPAGLVALWREGLLAQKVLLGQTKGYRAHPQLMRFAAMPDPVLA
                     IGCYLSEVVAEAGRRGYRFDATKIVRTGPYPVMRVTTGQMAYEWTHLLTKLSLRSPTL
                     HEKRKNIREPDPHPLFAVVPGPVEDWERV"
BASE COUNT          385 a          682 c          671 g          369 t
ORIGIN      
        1 cggcgatcag gcccatatag ggaccgacca gcttggtcgc cacgcgatag ccgccgccgc
       61 cggtcggaaa cagttcgatg acctggttgt aggccagcgc gatgatgaag actgtcgcgg
      121 cggtggcaaa ggcgaggtac agcccgagat cggtgttctc gccgagcgcg cggaacgtgt
      181 attcaggacc gtaggcggaa gacgagaggc cgtccgctcc caggccgacc caggcgaaga
      241 atgcgaccag cgccagcgag tgccgggtct cgggcttgag cggatcgagc gccttgccga
      301 gcacgagttc gcggaattta cctgacttca tggagcgcct gccgcgaggc aaggattgcg
      361 gagaagccgc gatgatacgc ctgtgcgcag aaaactgtgc agtgcgctat gacgcggcga
      421 cggtgacgct ggccggattg actggcacac cctcggttgc cggttctcca tgaatcggcg
      481 ataatgcgcg cttgtctgtc gccgagatgg cggaacaggt agacgcacgg gactcaaaat
      541 cccgcgccgc aaggcgtgcc ggttcgattc cggctctcgg caccagcgtg acaggcaacg
      601 caatgacgca cccggcacgg tgcgtttttt tatgcccgga cggcaagccg tctgtgtccg
      661 tgcgtgaaat cggcacctgc ttataatcag ctcacgcgat caccgccgca catccccatc
      721 atgcccgtat ccctcgacaa tctgctggtc gtcggcattt cctcgcgtgc cctgttcgac
      781 ctcgaggcgg aggaggccat cttccgcaca caggggctgg aagcctatcg ccgccaccag
      841 gtcgagcatg aaaacgaaat tctgaaactg ggtggcggtt tcgccctggt gcgcgcactg
      901 ctcaagctga accagctcgc gcacaaccag cgcttcgtcg aagtcgtgat catgtcgcgc
      961 aattcgaccg agacttcgct gcgcattttc aattcgatca agcattacgg gctcgacatc
     1021 acacgggccg tgctgtcggg cggcgcgccg ctggcgccgt atctgcgcgc gttcaacgtg
     1081 agcctgttcc tgtcgctgca cgaggacgac gtgcagctgg cgatcgattc cggcgtggct
     1141 gccgccttgc tctaccagaa accggacaac gcgttcgagg aactggacca gatccgcatc
     1201 gccttcgacg gcgacgcggt gatcttctcc gacgagtccg agcgcatctt tcaaacgcag
     1261 ggcatcgagg ccttcgagca ccatgaacgc gagaacgcgc tgcgcccgct gcctgaagga
     1321 ccgttcgccc gcctgctgaa ggccttgtcc ttcatccaga acaacctgaa gacggccgac
     1381 ggcggcaagg cgccgatccg caccgcgctg gtcaccgcgc gatcctcgcc ggcgcatgaa
     1441 cgcgtgatcc gcacgctgcg tgcctgggat gtggcgatcg acgagacctt cttcatgggc
     1501 ggcgtggcca agcaggatgt gctggccgcg ttcaagccgc atatcttctt cgacgaccag
     1561 cagggccatt gcgagcgggc cgcgccgctc gtgcagaccg gacgcgtgcc ggtcaagagc
     1621 atcggctagt ttttcgccgc gccgggaaca gggctcctga gcgcatatcg cgcgggggca
     1681 ggatcggcaa aggcaccccg gccgctcgcg ctcacacgcg ctcccagtcc tctaccggcc
     1741 cgggaacgac ggcaaaaagg ggatggggat ccggctcccg aatattcttg cgcttttcat
     1801 gcagtgtggg cgagcgcagg ctcagcttcg tgagcagatg cgtccactcg tatgccatct
     1861 gcccggtggt cacgcgcatg accggatatg gtcccgtgcg cacgattttc gtcgcgtcga
     1921 accggtagcc gcgccgccct gcttctgcga cgacctcgga aaggtagcag ccgatcgcaa
     1981 gcacgggatc gggcatcgcg gcgaagcgca tcagttgcgg atgtgcccga taccctttcg
     2041 tctgccccag caggaccttc tgcgcgagca gtccttcgcg ccagagcgcc acgaggcccg
     2101 ccggatc
//
LOCUS       DATLGF010000072         2540 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_191547, whole genome shotgun sequence.
ACCESSION   DATLGF010000072 DATLGF010000000
VERSION     DATLGF010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191547"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VM406_02165"
     CDS             <1..648
                     /locus_tag="VM406_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HVL74795.1"
                     /translation="WGRFKARTRPTPGNHEYYLPHAGAYFAYFGAAAGPARRGYYSFG
                     AGTWRVVSLNSNLRPADHRLQLDWLAEELKANPARCILAFWHHPRFSSGEHGDHAQMT
                     RAWTLLQAAGADVVLSAHEHLYERFAPQDADGRRNEAHGIRQFVVGTGGAPLDPFRGT
                     KPNSEARNSDVHGVLKLTLRDGGYDWEFLAVEPDRYTDRGSGACKAAPSPSGDRP"
     gene            645..1199
                     /gene="thpR"
                     /locus_tag="VM406_02170"
     CDS             645..1199
                     /gene="thpR"
                     /locus_tag="VM406_02170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HVL74796.1"
                     /translation="MNQHTGPMQRLFLALWPDDDTRNALSALQAGARGRLVPYADLHL
                     TLVFLGQQPLASLPDLTDVLAHLPRGEIEMRLDKVGYFTRNRILWAGSHSLPPALPEL
                     HKFMMDALAERGVAWKEDGREYSPHITLARDADAPMDLVFEPFVWRARHVALVRSNSQ
                     PQGSRYEVIASRMLDEDVRLPDPG"
     gene            complement(1200..2033)
                     /gene="tcdA"
                     /locus_tag="VM406_02175"
     CDS             complement(1200..2033)
                     /gene="tcdA"
                     /locus_tag="VM406_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HVL74797.1"
                     /translation="MNAIVSLPDLPVLDGNETPFAIEHDIDFERRFGGVARLYGDAAL
                     ARFGNARVCVVGVGGVGSWVVEALARSAIGNITMIDLDNVAESNINRQLHALSSTLGK
                     AKVSALAERIAQINPYCRVTEIEDFVAEDNLEQFIGAGRFDFVIDAIDNVRAKTALIV
                     YCRAKGIRLVTIGGAGGQIDPTRIEVRDLARTEQEPLLAKVRKRLRAQHGFPRGDKSR
                     FNIDAVFSTEPLRMPQGACATGDAVTGLNCAGFGSSMAVTASFGLVAAAQVLRKLADE
                     A"
     gene            2148..>2540
                     /locus_tag="VM406_02180"
     CDS             2148..>2540
                     /locus_tag="VM406_02180"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL74798.1"
                     /translation="MLPSAPLSLVILVSLLGHLALAGTRVTTSLYALSLHASAFTIGT
                     LIAVFALFPMLLAVPAGRLVDRVGVVRPLAAGAAAVLIGCLLAAAAGDLTLLYPAAAL
                     IGTGFMAIQIAAQHMVGTMSTSEKRAANF"
BASE COUNT          414 a          885 c          808 g          433 t
ORIGIN      
        1 tggggccgct tcaaggcgcg cacccgcccc acccccggca accacgagta ctaccttcct
       61 cacgccggcg cgtacttcgc ctattttgga gcggccgcgg gcccggcgcg gcgcggctac
      121 tacagtttcg gcgccggcac ctggcgcgtc gtctcgctca acagcaacct gagacccgcc
      181 gaccaccgtc tgcagctcga ctggctggct gaagaactca aggccaatcc ggcacgctgc
      241 atcctcgcct tctggcacca cccgcgcttc agttccggcg aacacggcga ccatgcccag
      301 atgacgaggg cctggacatt gctgcaggca gcgggagcgg acgtcgtcct gtcggcgcac
      361 gaacatctct atgagcgctt cgccccgcag gacgccgacg gccggcgcaa cgaagcgcat
      421 gggatccgac agttcgtggt aggcaccgga ggggcgcccc tggatccgtt tcgcggcacc
      481 aagcccaaca gcgaggcgcg caacagcgac gtacacggcg tgctcaagct cacgctgcgc
      541 gatggcgggt acgattggga attcctggcg gtcgaaccgg accgctacac cgaccgcggc
      601 agcggcgcat gcaaggccgc gccatcacca tccggagatc gcccatgaac cagcacaccg
      661 gtccgatgca gcgcctgttc ttggcgctct ggccagacga tgacacgcgc aatgcgttaa
      721 gcgcactgca ggccggcgcg cgcgggcgcc tggtgccgta cgcggacctg cacctgacgc
      781 tggtctttct tggccagcag ccgctcgcca gcctgcccga cctcacggac gtgctggccc
      841 accttccgcg cggcgagatc gagatgaggc tggacaaggt cggctacttc acccgcaacc
      901 gcatcctgtg ggcaggcagc cacagcctgc ctcctgcgct gccggagctg cacaaattca
      961 tgatggatgc actggccgaa cgcggcgtgg catggaagga ggacgggcgc gaatacagtc
     1021 cgcacatcac gctcgcgcgc gacgccgacg cgccgatgga cctggtgttc gagccgttcg
     1081 tctggcgcgc gcgccacgtc gcgctggtgc gctcgaacag ccagccgcag gggtcgcgct
     1141 atgaagtgat cgccagccgc atgctcgacg aagacgtgcg ccttcccgat cccggctgat
     1201 caggcctcgt cagccagctt gcgcaacacc tgggcggcgg cgaccaatcc gaagctggcc
     1261 gtcaccgcca tcgaagagcc gaagccggcg cagttcaggc cggtgactgc gtcgccggtt
     1321 gcgcaggcgc cttgcggcat gcgcagcggc tcggtcgaga acaccgcgtc gatgttgaac
     1381 ctgctcttgt cgccgcgcgg gaagccatgc tgcgcgcgca agcgcttgcg caccttggcc
     1441 agcagcggct cctgttcggt gcgcgcgagg tcgcgcactt cgatacgggt cgggtcgatc
     1501 tgcccgccag ccccgcctat cgtgaccagg cgaatgccct tggcgcggca gtacacgatc
     1561 agtgcggttt ttgcgcgcac gttgtcgatt gcgtcgatga cgaaatcgaa gcggccggcg
     1621 ccgatgaact gctcgaggtt gtcttccgcg acgaaatcct cgatctcggt gacgcggcaa
     1681 tacggattga tctgcgcgat gcgctcggcc agcgcgctga ccttggcctt gccgagcgtg
     1741 ctgctgagcg catggagctg gcggttgatg ttggactctg caacgttgtc caggtcgatc
     1801 atcgtgatgt tgccaatcgc gctgcgtgca agcgcctcta ccacccagga gccgaccccg
     1861 cctacgccga ccacgcacac ccgcgcattg ccaaagcgcg ccagtgccgc atccccgtac
     1921 aggcgtgcga cgccgccgaa gcgccgttcg aaatcgatgt catgttcgat tgcaaacggg
     1981 gtttcgttgc catcaaggac aggcaggtcg ggcaaggaga caatcgcgtt catcgcgcca
     2041 ttttaacgga gggaaacagc aaagttttcg catagaatcg ttttcggtgc cgaagttcaa
     2101 cgtatcgtgc gatgcaatgg tcggcgccgt tttccacccc gtttcgcgtg cttccctccg
     2161 ccccgctttc tcttgtgatc ctggtctccc tgctcggcca tctggcgctg gccggcaccc
     2221 gggtcaccac ttcgctgtac gcgctgtcac tgcatgcgtc cgcatttacg atcggcacgc
     2281 tgatcgccgt gttcgcgctg ttcccgatgc tgctggccgt gcctgcggga cgcctggtcg
     2341 accgcgtcgg cgtggtcagg ccgctggccg ccggcgcggc ggcagtgctg atcggctgcc
     2401 tgctcgcggc cgccgccggc gacctgaccc tgctctaccc cgccgcggcc ctgatcggca
     2461 ccggcttcat ggcgatacag atcgccgccc agcacatggt cggcacgatg agcacgagcg
     2521 agaagcgcgc ggccaacttc
//
LOCUS       DATLGF010000073         5022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_35098,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000073 DATLGF010000000
VERSION     DATLGF010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35098"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VM406_02185"
     CDS             <1..807
                     /locus_tag="VM406_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HVL74799.1"
                     /translation="VLERGRMEKRAAFGLKPEQTNRVDFNFYVEDDVVTILRRKRGAP
                     LDKIVSELMIFANSTWGKLMHDHGVPGIYRTQGASGGWPGRMQVRMLTHAAPHQGLGV
                     DQYAWSTSPLRRYTDLVNQWQILACVEHGVTAPLVAPFKPKDADLFAIVSAFDAAYSA
                     YAEFQSNMERYWCLRWLAQENARHVNAVVLKDEMLRLVDIPLVIRLTGMPPTARGTEV
                     RLELIRWDEVDLNVEARLIEITSDQEATAAGSTEETPTADSEADAESLDE"
     gene            916..1812
                     /locus_tag="VM406_02190"
     CDS             916..1812
                     /locus_tag="VM406_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HVL74800.1"
                     /translation="MKLFLENRLLSAAFGVSLAVHGMLLAVRFTAPEAFNLKPADPGL
                     EVILVNAKHDRAPLKADALAQANLDGGGNADSGRSKSPLPDSGRHADGDSATAMQRRI
                     EQLEEQQRMLAQLSKKSPDTLRAPADAVRERQARPQQGTDANETQRELTRQAAEIARR
                     IEDENKRPKKTFISPSTRQVGYAMYFKSVSERIEKFGTLNFPQHNGRKLYGELTVSIS
                     IFQDGRIYDKDPNQGITIDRSSGNTVLDEAARRIVRQSAPFGAFPNNMRTSGKDDVWV
                     MTTRFKFTRDGELETEMRGGPG"
     gene            1809..2624
                     /gene="aroE"
                     /locus_tag="VM406_02195"
     CDS             1809..2624
                     /gene="aroE"
                     /locus_tag="VM406_02195"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HVL74801.1"
                     /translation="MNVDRYGVIGNPVAHSKSPEIHAAFAAQTGQALSYERVLAPLDG
                     FADTVARLAREGYKGANVTVPFKLEALALCDTLTPRARTAGAVNTLSFDGGQIHGDNT
                     DGIGLVTDIVRNAGVELAGRQILLLGAGGAVRGVLLPLLEQKPARVVIANRTVARAEE
                     LAETFAQYAQVAASSFAALDTPFDVVINGTAASLAAELPPVPPAVFATGGYAYDMMYA
                     KEPTIFLQFAARSGVRVRDGLGMLVEQAAEAFFVWRGVRPDTAPVYHALRAAL"
     gene            2625..3320
                     /gene="mtgA"
                     /locus_tag="VM406_02200"
     CDS             2625..3320
                     /gene="mtgA"
                     /locus_tag="VM406_02200"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HVL74802.1"
                     /translation="MKQLRRLLLWAALLPIALVLLLQLYFLAQILWWKHFNPQSTSFM
                     KRQLAMLREKDPKATLKHQWVPYDSISIHLKRAIIAAEDANFSSHDGIDWTALQKAFE
                     KNQRKGRVVAGGSTITQQLAKNLFLSGERSYVRKAQEVVITYMLEYTMSKERIFEIYL
                     NVAEWGVGVFGAEAAARHYYGVSAARLSRYQAARLAVMLPQPRFYDRNRNSAYLARRT
                     DLILRRMQAAELP"
     gene            3476..4354
                     /locus_tag="VM406_02205"
     CDS             3476..4354
                     /locus_tag="VM406_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HVL74803.1"
                     /translation="MINVFVLENGRLNQVNIDSREDLIKVQPVWVDLTDPSDEERAWV
                     RSIYDVALPGEDEVKDIEASARYYEAGNGDLHLRSDFLLEEWDGPSQTVTVAFILSRN
                     ILFSVHASDLPIFRLVRLRARSRPGSIVDYKDVLLDLYATDAEAAKALNAIAVQEDLN
                     GRIRRDMMDTRRAISYLMRGRVLNADQFEEARQIMRDIESLDGHTAFLFDKINFLMEA
                     TVGFININQNKIIKIFSVSAVAFLPPTLIASVYGMNFRVLPELDWSFGYPFSIALMVI
                     SAVTPFWYFRKRGWLN"
     gene            complement(4519..>5022)
                     /locus_tag="VM406_02210"
     CDS             complement(4519..>5022)
                     /locus_tag="VM406_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVL74804.1"
                     /translation="ITTLGKVIGGGLPVAAFGASREIMKHLAPLGPVYQAGTLSGNPV
                     AVAAGMATLKLIQEAGFYERLSAQTGKLAAGLSAAAKDAGVSFCADAVGGMFGLYFAD
                     KVPGSYAQMMACDKERFNRFFHAMLERGVYLAPSAFEACFVSAAHDDAVIAATIDAAS
                     EAFQSIA"
BASE COUNT          940 a         1646 c         1577 g          859 t
ORIGIN      
        1 gtgctcgagc gcggccgcat ggaaaaacgc gcagcgttcg gactcaagcc ggagcagacc
       61 aatcgcgtgg acttcaactt ctacgtcgag gacgacgtcg tcaccatcct gcggcgcaag
      121 cgcggcgcgc cgctggacaa gatcgtgtcc gagctgatga tcttcgccaa cagcacctgg
      181 ggcaagctga tgcacgacca cggcgtgccc ggcatctacc gcacgcaagg cgcgtccggc
      241 ggctggccgg gacgcatgca ggtgcgcatg ctcacccacg cggcgccgca tcagggcctc
      301 ggcgtcgatc aatatgcatg gagcacctcg ccgctgcgcc gctacaccga cctggtcaac
      361 cagtggcaga tccttgcctg cgtcgaacac ggcgtgaccg cgccgctcgt cgcgccgttc
      421 aagccgaagg acgccgacct gttcgcgatc gtttcggcat tcgacgccgc ctattcggcc
      481 tatgccgaat tccagtccaa catggaacgt tactggtgcc tgcgctggct ggcgcaggag
      541 aacgcgcgcc atgtcaacgc ggtggtgctc aaggacgaaa tgctgcgcct ggtcgacatt
      601 ccgctggtga tccggctgac cggcatgccg ccgaccgcgc gcggcaccga ggtgcggctg
      661 gagctgatcc gctgggatga agtcgatctg aacgtcgaag cgcgcctgat cgaaattaca
      721 agcgatcagg aagcgaccgc ggccggcagc actgaagaaa cgccaaccgc tgacagcgag
      781 gcggacgccg aatcattgga cgagtgacac gcgtggcggg aataccgcga gattcggtaa
      841 cagaaggctc atctttgccg gcttgcggtt ttctttgacg taaaattcgc tgcgtccaac
      901 ccgaagtctg tccgcgtgaa gctttttcta gaaaaccgcc tgttgagtgc cgcgttcggg
      961 gtgtccctgg ctgtgcatgg catgctgctc gcggtgcgct tcacggcgcc cgaggcattc
     1021 aatctgaagc cggccgatcc cggtctcgaa gtcatcctcg tcaatgccaa gcatgaccgc
     1081 gcgccgctca aggctgacgc gctggcgcaa gccaatctcg acggcggcgg caacgccgac
     1141 agcgggcgct cgaagtcgcc cctgccggat tccggccgcc atgccgacgg cgacagcgcg
     1201 acggccatgc agcgtcgcat cgagcagctg gaagaacagc aacgcatgct cgcgcagctg
     1261 agcaagaaga gtcccgatac gctgcgcgcg ccggccgatg ccgtcaggga gcgccaggcg
     1321 cggccgcagc agggcacgga cgccaacgaa acgcagcgcg agctaacgcg gcaggcggcc
     1381 gagattgcca gacgcatcga ggatgagaac aagcgtccga aaaagacctt catttcgccc
     1441 agcacccgcc aggtcgggta tgcgatgtat ttcaagagcg tcagcgaacg catcgaaaaa
     1501 ttcggcacgc tcaacttccc gcagcacaat ggcaggaagc tgtacggcga gctgacggta
     1561 tcgatttcga tcttccagga cggccgcatc tacgacaagg acccgaacca ggggatcacg
     1621 atcgaccggt cgtccggcaa caccgtgctc gacgaagcgg cgcgccgcat cgtgcgccag
     1681 tcagcaccat tcggcgcgtt cccgaacaac atgcgcacca gcggcaagga tgatgtctgg
     1741 gtcatgacca cccgcttcaa gttcacccgc gacggcgagc tggagacgga aatgcgcgga
     1801 ggaccgggtt gaacgtcgac cgatatggcg tgatcggcaa tcccgtcgcg cacagcaagt
     1861 ccccggaaat ccatgccgcc tttgccgccc agaccggcca ggcgctgagc tacgagcgtg
     1921 tgttggcgcc gctggacggg ttcgccgaca ccgtcgcgcg gttggcccgg gaaggctaca
     1981 agggcgcaaa tgtcacggtg ccgttcaagc tcgaggcact ggcactgtgc gatacgctca
     2041 cgccgcgcgc gcgcacggcg ggggcggtca ataccttgag cttcgacggc gggcagatcc
     2101 acggcgacaa taccgacggc atcggcctgg tgacggatat cgttcgcaac gccggggtcg
     2161 agctggcggg ccggcagatt ctgctgctcg gtgccggcgg ggctgtgcgg ggtgtgctgc
     2221 ttccgctgct cgaacagaag ccggcccggg tcgtgatcgc caaccgcacg gtggcccgcg
     2281 ccgaggagct ggccgagaca tttgctcagt acgcccaagt tgcagcgagc agcttcgcgg
     2341 cgctcgacac gcctttcgat gtcgtgatca acggcacggc ggccagcctt gctgccgaac
     2401 tgccaccggt tccccctgcg gtgttcgcca ctggcggata cgcctacgac atgatgtatg
     2461 cgaaggagcc gaccatcttc ctgcagtttg ccgcgcgcag cggcgtgcgt gtgcgcgacg
     2521 gtcttggcat gctggtcgaa caggctgccg aagccttctt cgtctggcgc ggcgtgcgcc
     2581 cggatacggc gcccgtctac catgcgctgc gcgcggcgct ctgaatgaaa cagctgcgca
     2641 ggctgctgct gtgggcggcg ttgctgccga tcgcgctcgt gctcctgctg cagctgtatt
     2701 tcctcgcgca gatcctttgg tggaagcatt tcaatccgca gtcgaccagc ttcatgaagc
     2761 gccagctcgc aatgctgcgc gagaaggacc cgaaggcgac gctcaagcac cagtgggtgc
     2821 cgtacgacag catctccatc catttgaagc gcgcgatcat cgccgcggag gacgccaatt
     2881 tttccagcca cgacggcatc gactggactg cgctgcagaa ggcgtttgaa aagaatcagc
     2941 gcaagggccg cgtcgtggcc ggcggatcca cgatcaccca gcaactcgcg aaaaacctgt
     3001 tcctttccgg cgagcgcagc tacgtgcgca aggcccagga agtggtgatc acctacatgc
     3061 tcgaatacac gatgagcaag gaacgcatct tcgagatcta cctgaacgtc gccgaatggg
     3121 gtgtcggggt attcggagcg gaggccgctg cgcgtcacta ctacggcgtg tcagccgcac
     3181 gcttgtctcg ctatcaggcc gcccgactcg cggtcatgct gccgcagccg cgcttctatg
     3241 acaggaatcg caactcggcc tacctcgcgc gccgcaccga cctgatcctc aggcgcatgc
     3301 aggcggccga gttgccatga tcgtgcaacg cggtgcgcgc atagcgccgg tgcggcggga
     3361 tcggggcctt cccgccttgc tagtgcactg cggtatcagt aaacttgcgt ccggctccgg
     3421 cgccagaggc cggggcggtg cgccgcgctc gcgtcgtgcc tgaccgggaa agcgcatgat
     3481 caacgtattc gtcctcgaaa acggacgcct caatcaagtc aacatcgatt cccgcgagga
     3541 cctgatcaag gtgcagccgg tatgggtgga tctgaccgat ccatccgacg aggagcgggc
     3601 ctgggtccgg agcatctacg acgtggcgct gcccggcgag gacgaagtca aggacatcga
     3661 ggcgtcggcg cgctattacg aagccggcaa tggcgacctc cacctgcgca gcgacttcct
     3721 gctggaggaa tgggacggcc cctcccagac ggtcacggtg gcgttcatcc tctcgaggaa
     3781 catcctgttc tcggttcacg cgagcgacct gccgatcttc cgcctggtcc gcctgcgagc
     3841 gcgttcgcgt cccggctcca tcgtcgacta caaggatgtg ctgctggatc tgtacgctac
     3901 cgacgccgag gcggccaagg cgctcaatgc catcgcggtc caggaagacc tgaacgggcg
     3961 catccggcgc gacatgatgg atacgcggcg cgcgatcagt tatctgatgc gcggccgcgt
     4021 cctcaacgcc gaccagttcg aggaagcgcg ccagatcatg cgcgacatcg aatccctgga
     4081 cggtcacacg gccttcctgt tcgacaagat caacttcctg atggaagcga cagtcggctt
     4141 catcaacatc aaccagaaca agatcatcaa gatcttttcg gtctccgccg tcgccttcct
     4201 gccgcccacg ctgatcgcga gcgtctacgg catgaacttc cgcgtgctgc cggaactcga
     4261 ctggagcttc ggctatccgt tctcgattgc gctgatggtg atctctgccg tcacgccgtt
     4321 ctggtatttc cgcaagcgcg gctggttgaa ctgaaaccgg cgccgttatg gcgctgtttc
     4381 gtcagccggc catcgcctgt ttactgtcgt cccgaatgta gtgagggatc tcatccgttt
     4441 gccaagcgac ggcgtacagc aagcagatga ggttcccccg cttcgctcgg aacggcggca
     4501 ttggatccat cgcctgtctt aagctatcga ctggaacgcc tcgctcgccg catcgatcgt
     4561 cgccgcgatc accgcatcat catgtgcagc cgacacgaag cacgcctcga atgccgacgg
     4621 cgccaggtag acgccccgtt ccagcatcgc gtggaagaag cggttgaagc gctccttgtc
     4681 gcaagccatc atctgggcgt acgagcccgg caccttgtcc gcgaagtaca ggccgaacat
     4741 gccgcccacc gcatccgcgc agaagctcac acccgcatcc ttcgcggcgg cgctgagccc
     4801 cgcggcgagc ttgccggtct gcgccgaaag acgctcgtag aaacccgcct cctggatcag
     4861 cttcagggtc gccattcccg ccgcgaccgc gaccggattg cccgatagcg tgcccgcctg
     4921 atagaccggg ccgagcggcg caaggtgctt catgatttcc cggctggccc cgaacgcggc
     4981 caccggcagg ccgccgccga ttaccttgcc cagcgttgtg at
//
LOCUS       DATLGF010000074         4920 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_191602, whole genome shotgun sequence.
ACCESSION   DATLGF010000074 DATLGF010000000
VERSION     DATLGF010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191602"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1861)
                     /locus_tag="VM406_02215"
     CDS             complement(62..1861)
                     /locus_tag="VM406_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HVL74805.1"
                     /translation="MRKFLAPLLLLGLLQHGSSALAAHAFSLYDTPKYPAGFSHFEYV
                     NPNAPRGGELYLANPDRRTSFDKFNPFSMKGVSAAGVANLMFETLATQSADEVATMYG
                     LLAEDMELAPDRMSMTFRLHPNARFNNGDPVTAEDVKHSFDTLIKFGAPQFKLLYSDV
                     ERCVVVDERTVRFDFKELNRELPLIVGGTPVFSKKWGANTTFDRIQLEAPIATGPYLI
                     ERYDTGRSITFRRNPDYWGNDIAVRRGMFNFERIVYRFYKDDVARLEAFKAGEFDMVV
                     ENSAKNWARAYVGRQFDNGEIVKRELEHSNGAGMQGFIMNTRRPQFQDVRVRQALAYA
                     LDYEWMNRQLFYKQYTRIYSYFNNSEMAAKGLPSEAELKLLEPLREQLDPVVFGPAPV
                     PPNTDPPGSLRANLLKARELFREAGWEYRDGALRNKAGQPFTFEIMDDQGAMSRVVSV
                     YVRNLRILGVDVRQRTADFTLVQKRMQDFDFDMTSVRFPDTTSPGNEMFDMFGSQAAD
                     ETGSSNYWGLKDPAVDKLVSTLVNAETRADLVAAARALDRVLLHKHLVVTHWYSSTHR
                     VAYRNRFGMPDKPPLYYTADPYVISTWWQVKPR"
     gene            2186..2968
                     /gene="fabI"
                     /locus_tag="VM406_02220"
     CDS             2186..2968
                     /gene="fabI"
                     /locus_tag="VM406_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HVL74806.1"
                     /translation="MAFLQGKKILITGLLSNRSIAYGIAQACKREGAELAFTYVGERF
                     KDRITEFAKEFDSSLVFDCDVSSDEQINALFADLGKSWDHLDGLVHSIGFAPREAIAG
                     DFLDGLSREAFRIAHDISSYSFPAMAKAALPMLRPGSALLTLTYLGSVRATPNYNTMG
                     LAKASLEASVRYLAESLGPKGVRVNGISAGPIKTLAASGIKDFSKLLGIVASHAPLRR
                     NVTIEDVGNTAAFLLSDLAGGITSEITYVDGGFSHVMCAPTE"
     gene            3456..4856
                     /locus_tag="VM406_02225"
     CDS             3456..4856
                     /locus_tag="VM406_02225"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HVL74807.1"
                     /translation="MSFEALGLHASILKALTEAGYTKPTPVQEQAVPAAMEGRDLLVS
                     SQTGSGKTAAFMLPALHRFALEEQNFVPGTKTPNQERQSARSRGERPRFTPAQPKMLV
                     LTPTRELALQVTTATTKYGSNLRRVRAVSILGGMPYPKQMQLLSRNPEILVATPGRLI
                     DHMESGKIDFSQLQMLVLDEADRMLDMGFIDDIEKIVAATPESRQTMLFSATLDGVVG
                     NMARRITKTPLVIQIAGSASKHENIQQRVHFVDDLSHKNRVLDHLLRDTSLDQAVVFT
                     ATKRDADTIADRLNINGFAAAALHGDMHQGARNRTLDALRRGQVRVLVATDVAARGID
                     VPGITHVVNYDLPKFAEDYVHRIGRTGRAGRNGLAISLVNHSETVNIKRIERFTKQLI
                     PVDVIEGLEPKKSAAPRSTRKPGGWKPGEGRRQGQGDRPGQRSFAKPGAPRKEGGGYG
                     KSRFADNGARRGYGER"
BASE COUNT          913 a         1608 c         1523 g          876 t
ORIGIN      
        1 cacccgatcc cggcccgcac acatcgccgc ctgaaaccgc tccgatgtgc caggcgccgt
       61 gttaccgcgg cttcacctgc caccaggtcg aaattacata cggatccgcc gtgtaataca
      121 gcggcggctt atccggcatg ccgaagcggt tgcgataggc gacgcggtgg gtcgacgaat
      181 accagtgggt cacgaccagg tgcttgtgca gcagtacgcg gtccagcgca cgcgcggcgg
      241 cgacgaggtc ggcgcgggtc tcggcgttga ccagcgtgga aaccagcttg tccacggcag
      301 gatccttgag cccccagtag ttgctcgagc cagtctcgtc tgctgcctgg gagccgaaca
      361 tatcgaacat ttcattgccg ggactggtgg tgtccgggaa gcgcacgctg gtcatgtcga
      421 aatcgaagtc ctgcatgcgc ttctgcacca gcgtgaagtc ggcggtgcgc tggcgcacgt
      481 cgacgccgag aatgcgcagg ttgcgcacat agacgctgac gacgcgcgac attgcgccct
      541 ggtcgtccat gatttcgaag gtaaacggct gccccgcctt gttgcgcagc gcgccgtcgc
      601 gatattccca gcctgcctcc cgaaacagtt cgcgcgcctt gagcaggttc gcacgcagcg
      661 agccgggcgg atcggtattc ggcggcaccg gcgcgggacc gaacacgacc gggtcgagct
      721 gctcgcgcag cggttcgagc agtttcagct cggcttccga cggcaggcct ttcgcggcca
      781 tctcgctgtt gttgaagtac gaatagatgc gcgtgtactg cttgtagaac agctgccggt
      841 tcatccactc gtagtcgagc gcgtaggcga gcgcctggcg cacgcgcacg tcctggaact
      901 gcggccggcg ggtattcatg atgaaaccct gcatgccggc gccgttggag tgttccagct
      961 cgcgcttgac gatctcgcca ttgtcgaact ggcggccgac ataggcgcgc gcccagttct
     1021 tcgcgctgtt ctcgaccacc atgtcgaatt cgccggcctt gaacgcctcc aggcgtgcaa
     1081 cgtcgtcctt gtagaagcgg tagacgatgc gttcgaagtt gaacatgccg cgccgcacgg
     1141 cgatgtcgtt gccccagtaa tcggggttgc ggcggaaggt gatcgagcgg ccggtgtcgt
     1201 agcgctcgat cagatagggc ccggtcgcaa tcggcgcttc gagctggatg cggtcgaacg
     1261 tcgtgttcgc cccccacttc ttcgagaaca ccggcgtgcc gccgacgatc agcggcagct
     1321 cgcggttgag ttccttgaaa tcgaatcgca cggtccgttc gtcgaccacg acgcagcgct
     1381 cgacatccga atacagcagc ttgaactgcg gcgcgccgaa cttgatcagc gtgtcgaacg
     1441 aatgcttgac gtcttccgcg gtgaccggat cgccgttgtt gaaacgcgcg ttcggatgca
     1501 gccggaaggt catcgacatg cggtccggcg ccagctccat gtcctcggcg agcaggccgt
     1561 acatggtcgc gacctcgtcc gccgactggg tggcgagcgt ctcgaacatc aggttcgcga
     1621 cgccggcggc ggagacgccc ttcatcgaga acggattgaa cttgtcgaag ctggtgcggc
     1681 ggtccggatt ggccaggtac agctcgcccc cgcgcggcgc gttcgggttc acgtactcga
     1741 agtggctgaa accggccggg tactttggcg tgtcgtagag agaaaacgcg tgggcggcca
     1801 gtgcgctgct gccgtgctgg aggagaccga gcagaagcag aggggcgaga aatttacgca
     1861 tgcacctgaa tggaaaagtg ctgttgggca tgggtgccga aaagtcgggc tattgtaccc
     1921 aaagggggct tccgcgcacg cattattttt ccccggatcg acgaacgttg caggacatta
     1981 agtacattta aaccttttat catggaagtt ggcggaagaa cttgattccc gccagaacct
     2041 tcgggaagtg ccgcagttgc cgggcctgcc atccacccct acaatcgcgc cagattgtgg
     2101 agcgctgaaa acgggagacc gctttcagcg cttcttgttg ttgtcggcgt cctgcgccgg
     2161 atcatgaaca catattggag tcaccatggc tttcctgcaa ggcaagaaga tcctgatcac
     2221 cggactgctg tccaaccgtt ccatcgccta cggcatcgcc caggcctgca agcgcgaggg
     2281 cgcggaactg gccttcacct atgtcggcga gcgcttcaag gaccgcatca ccgagtttgc
     2341 gaaagagttc gacagcagcc tggtgttcga ctgcgatgtc tccagcgacg agcagatcaa
     2401 cgcgttgttc gccgacctcg gcaaatcctg ggatcacctc gacggcctgg tgcactcgat
     2461 cggtttcgcg ccgcgcgagg caattgcagg cgacttcctc gacggtctgt cgcgcgaggc
     2521 cttccgcatc gcgcacgaca tctcctccta cagcttcccc gcaatggcca aggccgcgct
     2581 gcccatgctg cgccccggct cggccctgct gacgctgacc tacctcggtt cggtgcgcgc
     2641 caccccgaac tacaacacga tgggcctggc aaaggcatcg ctggaggcca gcgtgcgcta
     2701 cctggccgag tcactcggtc cgaagggcgt gcgcgtaaac ggcatctccg ccggcccgat
     2761 caagacgctg gccgcatccg gcatcaagga tttttccaaa ctgctcggca tcgtcgcctc
     2821 gcatgcgccg ctgcgccgca acgtcaccat cgaggatgtc ggcaacaccg ccgccttcct
     2881 gctgtccgac ctcgccggcg gcatcaccag cgagatcacc tatgtcgatg gcggcttctc
     2941 gcatgtgatg tgcgcgccaa cggagtgaga ctgccggatc tttcagtgaa gatttgaaca
     3001 acggcgggca cggcggacac ggcgaggact tgtcattccg agcccagcga ggaatctcga
     3061 ttctgcgatc ggatgagatc cctcgctgcg ctcgggatga cagtgagttg ggtttttcgc
     3121 cgtgttcgcc gtgtccgccg tggttcaaac gaaaacacct ccgcacacca aacgctccag
     3181 catagagaaa tcccgcacgc aggcgcacat ctctgtataa tgcgccgctg tgccacgcaa
     3241 accgtggtcg atcgcagcat cgcaaaccgt ttagcagttc tcaaaaagcc atcccgcctc
     3301 ctggtgtcgc tctcttactg acaccgtccc ggcgcaaagc ttttgtgccg aaatctctca
     3361 gattcccgtc tcagctgttt cgctggttgg ctttgccctt ttatccgtcc gcacctgtca
     3421 cggacagtcg cggcgtcgaa tcaagaaaga aatacatgtc atttgaagca ctaggcctgc
     3481 acgcgtccat cctcaaggca ctgaccgaag ccggctacac gaaacccacc ccggtacagg
     3541 aacaggccgt tcccgccgcg atggaaggcc gggacctgct ggtctcctcg caaaccggtt
     3601 ccggcaagac cgcagcgttc atgctgccgg cgctgcaccg ctttgcgctc gaagagcaga
     3661 acttcgtgcc gggcacgaag actccgaacc aggaacgcca gtcggcgcgt tcgcgcggcg
     3721 aacgtccgcg cttcacgccg gcccagccga agatgctggt gctgacgccg acccgcgagc
     3781 tggcgctgca ggtcaccacc gcaaccacca agtacggcag caatctgcgc cgcgtgcgcg
     3841 ctgtctcgat cctgggcggc atgccgtatc cgaagcagat gcaactgctc tcgcgcaatc
     3901 cggaaatcct ggttgcaacc cccggccgcc tgatcgacca catggagtcg ggcaagatcg
     3961 atttctcgca gctgcagatg ctggtcctcg acgaggccga ccgcatgctc gacatgggct
     4021 tcatcgacga catcgagaag atcgtcgcgg cgacgcccga gtcgcgccag accatgctgt
     4081 tctccgccac gctcgacggc gtggtgggca acatggcgcg ccgcatcacg aagacgccgc
     4141 tggtgatcca gatcgccggc tcggcttcga agcacgagaa catccagcag cgcgttcact
     4201 ttgtcgatga tctctcgcac aagaaccgcg tgctcgacca tctgctgcgc gatacatcgc
     4261 tagaccaggc cgtcgtgttc acggcgacaa agcgcgatgc ggacacgatc gccgaccgcc
     4321 tgaacatcaa cggctttgct gccgccgccc tgcatggcga catgcaccag ggcgcacgca
     4381 accgcaccct cgacgcgctg cgtcgcggcc aggtgcgcgt gctggtggcc accgacgtgg
     4441 ccgcgcgcgg catcgacgtg ccgggcatca cccacgtggt gaactacgac ctgccgaagt
     4501 tcgcggaaga ctacgtgcac cgtatcggcc gcaccggccg tgccggccgc aacggactgg
     4561 cgatctcgct ggtcaaccac agcgaaaccg tcaacatcaa gcgcatcgag cgcttcacca
     4621 agcagctgat tccggtcgac gtgatcgagg ggctggagcc gaagaagagc gcagcgccgc
     4681 gcagcacccg caagccgggc ggctggaagc cgggcgaggg ccgccgccag ggccagggcg
     4741 accgtccggg ccagcgcagc ttcgccaagc cgggcgcgcc gcgcaaggaa ggcggcggct
     4801 acggcaagtc gcgcttcgcc gacaacggcg cacgccgcgg ctacggcgaa cgctgatcgc
     4861 cgaggccgcc gccttccttg cgcggcgcgc ccggcttggc gaagctgcgc tggcccggac
//
LOCUS       DATLGF010000075         2903 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_176339, whole genome shotgun sequence.
ACCESSION   DATLGF010000075 DATLGF010000000
VERSION     DATLGF010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176339"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VM406_02230"
     CDS             <1..436
                     /locus_tag="VM406_02230"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HVL74808.1"
                     /translation="LYKFMAYVEWPAGRAADGPLKIGVIGADEVAHELMRLVRLRGSQ
                     DRPVTVHRLRPTEAPTGLHALFIGGGYGQSPEPLLRQAQQESVLTVTENEQAFAHGSV
                     INFKLLDGRVRFDISLPAAQRHNLRLSSRLIAVAHEVHRREP"
     gene            433..2325
                     /locus_tag="VM406_02235"
     CDS             433..2325
                     /locus_tag="VM406_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL74809.1"
                     /translation="MIRSIATSVRRRLIAVILLTTAITLALTAGSVLLVETYIYERAR
                     VTELTGQAELLGLVSAAALRFDDRDAAAENLAMLHVRPRIMAAAIYDENGRLYAAYVN
                     PRHQGGVIPPQAGAPGHAVQASEIALFQPIVEQGERLGTIYLHAQFGLAERVAVYAAV
                     LAAALAGGMAIAWLLASRLQRSFTHPIYAITAAAKRVIATRDFSRRVTRSSNDEIGVL
                     VDAFNDMLHEVGLRTRALEEANRALAQEMEVRRGAEERLRQADRRKDEFLAMLAHELR
                     NPLAPISAAAQLLRLGRLDEERTRQTSELIARQVDHMTSLVNDLLDVSRVTRGLIILD
                     RKQQDLRRIASDAVDQVRPLLDMRGHRLHVALPDTPALVHGDDKRLVQVLANLLNNAA
                     KYTPTGGDIRLNVALLDEEVELSVIDNGIGMDPELQSHVFELFTQAERTSDRSQGGLG
                     LGLALVKSIVELHGGRVAAYSEGDGKGSAFSVRLPRSVSVAAPAAQPEQQAWRAAGGQ
                     ARVLVVDDNHDAADSLAAFLLAAGYDVHVSYDPFDAIERARVLVPQVCLLDIGLPGMD
                     GHALARRLRTLPGMADGLLIAVSGYGQPQDREAALAAGFDRHLVKPVDVPALARLLAE
                     RSPVAS"
     gene            2506..>2903
                     /locus_tag="VM406_02240"
     CDS             2506..>2903
                     /locus_tag="VM406_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL74810.1"
                     /translation="MRVLVVEDDASHAQFIADGLRRHGHHVDHARDGRDGLFLATEET
                     YDVLILDRMLPKLDGLSIVQTLRNAGNGTPALILSALGKVEERIKGLRAGGDDYLAKP
                     FEFGELLARIEALARRARPEQAEQVRQRVG"
BASE COUNT          471 a          971 c         1004 g          457 t
ORIGIN      
        1 cctctacaag ttcatggcct atgtcgagtg gccggcagga agggcggcgg acggaccgct
       61 gaagatcggc gtgatcgggg ccgacgaagt cgcgcacgaa ctcatgcgcc tggtcaggct
      121 gcgcggcagc caggaccggc cggtgacggt gcatcgcctg cgtccgacgg aggctccgac
      181 ggggctccat gcgctgttca tcggcggcgg ctacggccag agcccggagc ccctgctgcg
      241 gcaggcccag caggaaagcg tgctgaccgt gaccgaaaac gaacaggcgt ttgcccatgg
      301 cagcgtgatc aacttcaagc tgctcgacgg ccgcgtgcgc ttcgacatct cgctgccggc
      361 cgcgcagcgt cataacctgc gcctgagttc gcgcctgatc gccgttgccc acgaggtcca
      421 caggagggag ccatgatccg ttccattgcc acgtccgtcc gtcggcgcct gatcgcggta
      481 atccttctga ccaccgccat cacgctcgca ttgacggccg gatcggtgct gctggtggag
      541 acctatatct acgagcgggc gcgtgtcacc gaactgaccg ggcaggccga actgctcggt
      601 ctcgtgtcgg cggcggccct gcgcttcgac gaccgcgatg cggcggccga gaacctggca
      661 atgctccatg tgcggccgcg catcatggcc gccgcaatct acgacgaaaa cggccggctg
      721 tacgccgcct atgtcaatcc gcgtcatcaa gggggcgtca ttccaccgca ggccggcgcg
      781 cccggtcacg cggtgcaggc gtccgagatt gcgctgttcc agcctatcgt cgagcagggc
      841 gagcggctgg ggacgatcta cctgcatgcg cagttcggcc tcgccgagcg cgtggccgtc
      901 tatgccgcgg tgctggcggc cgccctcgcg ggcgggatgg cgatcgcatg gttgctggcc
      961 agccggctgc agcgcagctt cacccacccg atctatgcga ttaccgcggc ggccaagcgc
     1021 gtgatcgcca cccgcgattt ttcacgccgc gtcacccgca gttccaacga cgaaatcggt
     1081 gtgctcgtgg acgccttcaa tgacatgttg catgaagtcg gcctgcgcac ccgtgcgctc
     1141 gaggaggcga accgtgcgct ggcccaggag atggaggtca ggcgcggggc cgaggaaaga
     1201 ttgcgccagg ccgaccggcg caaggacgag ttcctcgcga tgctcgccca tgagctgcgc
     1261 aatccgctcg cgcccatcag tgcggcggcc cagttgctca ggctcggccg gctggacgag
     1321 gagcgcaccc gccagaccag cgaactgatc gcgcgccagg tcgaccacat gacaagcctg
     1381 gtcaacgacc tgctcgatgt gtcgcgcgtc acgcgcgggc tgatcatcct cgatcgcaag
     1441 cagcaggatc tgcgccgcat cgccagcgac gccgtcgatc aggtgcgtcc gctgctcgac
     1501 atgcgcgggc ataggctgca cgttgccttg ccggacacgc cggccctcgt gcatggcgac
     1561 gacaagcgcc tcgtgcaggt actggcaaac ctgctcaaca acgccgccaa gtacacgccg
     1621 acgggtgggg acatccggct gaacgttgcg ctgctggacg aggaggtgga gctcagcgtc
     1681 atcgacaacg ggatcggcat ggacccggag ctgcagtcac atgtgttcga gctgtttacc
     1741 caggccgagc gcacctcgga ccggtcccag ggcgggcttg gactcggact ggcactggtc
     1801 aagagcatcg tggagctgca cggcggacgc gtggccgcct acagtgaagg cgacggcaag
     1861 ggcagcgcct tctccgtgcg cctgccgcgc tccgtatcgg tggccgcgcc cgccgcgcag
     1921 ccggagcagc aggcgtggag ggcggccggc ggccaggccc gcgtcctggt cgtggacgac
     1981 aatcacgatg ccgccgactc cctggccgcc ttcctgctgg cggcaggata cgacgtgcat
     2041 gtgtcctacg atccgttcga tgcgatcgag cgcgcgcgcg tgctggtccc gcaggtctgc
     2101 ctgctcgaca tcgggctgcc gggaatggac ggtcatgcgc tggcgcgccg gctgcgcacg
     2161 ctgcccggca tggccgatgg gctgctgatc gccgtgagcg gatacgggca gccgcaggac
     2221 agggaggcgg cgctcgccgc cggcttcgac cggcacttgg tcaagccggt ggatgtaccg
     2281 gcgctggcgc gtctgctcgc cgagcggtcc ccggttgcgt cgtgaacgcc gcagcggccg
     2341 tcattgcaaa aatttgagac gggcgatgcc ggatcgcaca agcgaaaacg gctaaactgt
     2401 tcgctccgtt tgccggcccg cttgcgctct ttcccagcgc gggcgggccg cgtgcgttct
     2461 gcgggcgcaa acgatgcaat gcaatcaacg aggcagggag taccgatgcg agttctcgtc
     2521 gtggaagacg atgccagcca tgcgcaattc attgcggacg gcctgcgccg gcacggccac
     2581 catgtcgacc atgcgcgcga cggccgcgac ggcctgttcc tggcgacgga ggaaacgtac
     2641 gacgttctga tcctcgatcg catgctgccc aagctcgacg ggctttcgat cgtgcagacc
     2701 ctgcgcaacg ccggcaatgg cacgcccgcc ctgatcctca gcgcgttggg caaggtggag
     2761 gagcgcatca agggcctgcg ggccggcggt gacgactacc tcgccaagcc gttcgagttc
     2821 ggcgaactgc tcgcgcgtat cgaagcgctg gcgcgccggg cgcgtccgga gcaggccgag
     2881 caggtcaggc agcgcgtcgg cga
//
LOCUS       DATLGF010000076         4560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_160372, whole genome shotgun sequence.
ACCESSION   DATLGF010000076 DATLGF010000000
VERSION     DATLGF010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160372"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VM406_02245"
     CDS             complement(<1..304)
                     /locus_tag="VM406_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HVL74811.1"
                     /translation="MKKCDCRAYVIAGLMLATFATAVQAQYVWVDERGRKQFSDMPPP
                     ASVPAHRILKQPGVPAERPMAGDAAGGEPETRAPTPPAQQAEAPPTLAERDAEFRRR"
     gene            448..1908
                     /gene="guaB"
                     /locus_tag="VM406_02250"
     CDS             448..1908
                     /gene="guaB"
                     /locus_tag="VM406_02250"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HVL74812.1"
                     /translation="MRLLQKALTFDDVLLVPAFSQILPKDTSLTTRLTRNITLNIPLL
                     SAAMDTVTEARLAIAMAQEGGIGIVHKNLKPADQAREVAKVKRFESGVVRDPITIPPT
                     MRIREVIALSQQHGISGFPVVEGRKVVGIITNRDLRFEEELDAQVRDKMTPREKLVYV
                     DESAELAEAKRLMNKHRLERVLVVNDAFELRGLITVKDIQKATEHPNASKDDQGKLRV
                     GAAVGVGADNDERIDLLVKAGVDVIVVDTAHGHSKGVLDRVRWVKKNYPEVDVIGGNI
                     ATAAAAHALLEHGADGVKVGIGPGSICTTRIVAGVGVPQITAISNVAEALKGTGVPCI
                     ADGGIRFSGDIAKALAAGASSVMMGSMFAGTEEAPGEVILYQGRSYKSYRGMGSLGAM
                     SEGSADRYFQDAANNADKFVPEGIEGRVPYKGSVLAILYQLVGGVRSSMGYCGCSTID
                     EFREKAEFVEITAAGMRESHVHDVQITKEAPNYRAE"
     gene            1927..2955
                     /locus_tag="VM406_02255"
     CDS             1927..2955
                     /locus_tag="VM406_02255"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HVL74813.1"
                     /translation="MAPIEQAEGEAGTAGPDPGRFAVQWDDADPHIARISRLGRSLFK
                     VAGCHVSFDGLQGGEPDEGDAERAFFREVSLCDEPVVVRDTRLHLSLREHRAVTGAPH
                     IRFYAAYPLRGQHREPAGSISLVDYAPRPDFGFEQRRLLGDLAALVERELHLRSVSAT
                     QRDLQKQNKHLRRKSLVDPLIGTWNRGAIMRILSIEAVRCEKAGLPLSLIVADLDHFK
                     QINDRFGHPAGDAVLVKVASRLRSCIRPQEALGRYGGEEFLVVLPGASLPTALAIAER
                     MRAAVAGRPETVAGTTLALTISAGVASTETFASASMDELISRADVALYKAKDKGRNCV
                     VSAVSDLS"
     gene            2974..4554
                     /gene="guaA"
                     /locus_tag="VM406_02260"
     CDS             2974..4554
                     /gene="guaA"
                     /locus_tag="VM406_02260"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HVL74814.1"
                     /translation="MHSKILILDFGSQVTQLIARRVREAGVFSEVFPYDVSDEFVRSY
                     GAAGIILSGGPNSVTEGDTPRAPQAVFELGVPVLGICYGMQTMAEQLGGKVENGKVRE
                     FGYAEVRARGHTALLNGIADFVTDEGHGMLKVWMSHGDKIIEMPPGFKLMASTDNCPI
                     AGMADEERRLYAVQFHPEVTHTVQGKAMLGRFVHEICGCKSDWNMPDYISEAVEAIRK
                     QVGSDEVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLDEGTMVMNMFAKNL
                     GVKVIHVDATEQFMGKLAGVTDPEAKRKIIGREFVEVFQDEAGKLKNAKWLAQGTIYP
                     DVIESAGKGKKTAHTIKSHHNVGGLPETLNLKLLEPLRELFKDEVRELGVALGLPHDM
                     VYRHPFPGPGLGVRILGEVKKEYADLLRRADAIFIEELRAAKDEEGKSWYDKTSQAFA
                     VFLPVKSVGVMGDGRTYEYVVALRAVQTQDFMTAHWAHLPHDLLGRVSNRIINEVRGL
                     NRVVYDISGKPPATIEWE"
BASE COUNT          829 a         1399 c         1573 g          759 t
ORIGIN      
        1 gccgtctacg gaactcggca tcgcgctcgg ccagcgtggg cggggcctcg gcctgctgcg
       61 cggggggcgt cggcgcgcgc gtctcgggct cgccgcccgc cgcgtcgccg gccatcgggc
      121 gctccgccgg cactcccggc tgcttcagga tgcggtgcgc cggcaccgat gcgggcggcg
      181 gcatgtccga gaactgcttg cggcctcttt catcgaccca gacatactgc gcctgtaccg
      241 ccgtggcgaa tgttgccagc atcagtcccg cgatcacata agcccggcag tcacactttt
      301 tcatctgcat ttccgagtga agaacgagtg tcgatttcgc catgctttga tgatgatgtc
      361 aatcaagccg ctgcccataa gctgctgaaa aagcgacgtt tttaggcgcc ttctgtataa
      421 ttgcgctttg cgcctatagg aaacactatg cgtctactcc agaaagcact cacgttcgat
      481 gatgtgctgc tcgttccggc cttttcccag attctcccca aagacacctc cctgacaacg
      541 cgcctgacgc gcaacatcac attgaacatc ccgctgctgt cggcggcgat ggataccgtg
      601 accgaagcca ggctcgcgat cgcgatggcg caggagggag gcatcggcat cgtccacaag
      661 aacctgaagc cggcggacca ggcgcgcgaa gtggcgaagg tcaagcgttt cgagtccggt
      721 gtggtgcgcg atccgatcac gattccgccg acgatgcgca tccgcgaagt catcgcgctg
      781 tcgcagcagc atggcatcag cgggtttccg gtggtcgaag ggcgcaaggt ggtcggcatc
      841 atcacgaacc gcgacctgcg cttcgaggag gagctggacg cacaggtgcg cgacaagatg
      901 acgccgcgcg agaagctggt gtatgtggac gagagcgccg agctggccga ggcgaagcgt
      961 ctgatgaaca agcacaggtt ggagcgggtg ctggtggtca acgacgcgtt cgagttgcgc
     1021 ggcctgatca cggtgaagga tatccagaag gcgaccgagc atccgaacgc cagcaaggac
     1081 gaccagggca agctgcgcgt cggtgcggcg gtcggtgtgg gtgccgacaa tgacgagcgc
     1141 atcgacctgc tggtgaaggc gggtgtggac gtgatcgtgg tcgataccgc gcacggccat
     1201 tcgaagggcg tgctcgaccg cgtgcgctgg gtcaagaaga attacccgga agtggacgtc
     1261 atcggcggca acatcgccac cgcggcggcc gcgcacgcgc tgctcgagca tggcgccgac
     1321 ggcgtgaagg tcggcatcgg ccccggctcg atctgcacga cccgcatcgt ggccggcgtg
     1381 ggcgtaccgc agatcaccgc catctccaat gtcgccgagg ccctgaaggg caccggcgtg
     1441 ccctgcatcg ccgacggcgg catccgcttc tccggcgaca tcgccaaggc cctggccgcc
     1501 ggcgcctcga gcgtaatgat gggcagcatg ttcgccggca ccgaggaagc gccgggcgaa
     1561 gtgatcctgt accagggtcg cagctacaag tcgtaccgcg gcatgggcag cctgggcgcg
     1621 atgtccgaag gctcggccga ccgctatttc caggacgcgg caaacaatgc cgacaagttc
     1681 gtgcccgagg ggatcgaggg acgcgtgccg tacaagggca gcgtgctggc gatcctgtac
     1741 cagctggtcg gcggcgtgcg ttcgtcgatg ggctactgcg gctgctcaac gatcgacgag
     1801 ttccgcgaga aggccgagtt cgtcgaaatc accgctgccg gcatgcgcga atcgcacgtc
     1861 cacgacgtgc agatcacgaa ggaagcgccg aactatcgcg ccgagtaaat tctgcgcacg
     1921 cggctcatgg caccgatcga acaagcggaa ggcgaagccg gcactgctgg gccggatccc
     1981 gggcggttcg cggtgcaatg ggacgatgcc gaccctcaca tcgcgcgcat ctcgcgcctg
     2041 ggtcgcagcc tgttcaaggt ggccggctgc catgtcagtt tcgacggatt gcaaggcggc
     2101 gaaccggacg agggggatgc cgagagggca ttcttccgtg aggtgtcgct gtgcgatgaa
     2161 ccggtggtcg tgcgcgatac ccgtctgcac ctgtcgttgc gggaacaccg ggcggtgacg
     2221 ggcgcgccgc acatacgctt ctatgccgcc tatccgctgc gtgggcagca ccgggagccg
     2281 gccggcagca tcagcctggt cgactatgcg ccgcgtccgg atttcggttt cgaacagcga
     2341 cgtctgttgg gcgatcttgc cgcgctggtt gaacgcgagc tgcatctgcg gtcggtcagc
     2401 gcgacgcagc gcgatctgca gaagcagaac aagcacctgc ggcgcaaatc gctggtggac
     2461 ccgttgatcg ggacctggaa ccgcggcgcg atcatgcgca tcctgagcat cgaggcggtg
     2521 cgctgcgaga aggcgggtct gccgctgtcg ctgatcgtcg ccgatctcga tcacttcaag
     2581 cagatcaacg accgtttcgg ccatccggcc ggcgacgcgg tgctggtgaa ggtggcgagc
     2641 cggctgcgct cctgcatcag gccgcaggaa gcgctcggac gctatggcgg cgaggagttc
     2701 ctggtcgtgc tgccgggcgc gtcgctgccg accgcgctgg cgatcgcgga gcgcatgcgt
     2761 gccgctgtcg cagggcggcc ggagacggtc gcgggaacga cgctcgcgct gaccatcagc
     2821 gccggcgttg catcgaccga gacgtttgct tcggcaagca tggatgaact gatcagccgc
     2881 gccgacgtgg ctttgtacaa ggcgaaggac aagggccgca attgcgtggt cagcgccgta
     2941 tccgatttat cgtaatcctc cacacatact ttcatgcatt ccaagatcct gatcctcgat
     3001 ttcggctccc aggttaccca gctgatcgcg cgccgcgtgc gcgaggccgg cgtgttttcc
     3061 gaggtcttcc cgtatgacgt cagtgacgag ttcgtccgca gctacggcgc cgcaggcatc
     3121 atcctctccg gcggaccgaa ctcggtgacg gaaggggata cgccgcgtgc gccgcaggcg
     3181 gtattcgagc tcggcgtgcc ggtgctgggc atctgctacg gcatgcagac gatggccgag
     3241 cagctcggcg gcaaggtcga gaacggcaag gtgcgcgagt tcggctacgc ggaagtgcgc
     3301 gcacgcggcc ataccgcgct cttgaacggc atcgccgatt tcgtcacaga cgaaggccac
     3361 ggcatgctga aggtctggat gagtcatggc gacaagatca tcgagatgcc gccgggcttc
     3421 aagctgatgg cgtcgaccga caactgcccg atcgccggca tggccgacga ggaacgccgc
     3481 ctgtatgcgg tgcagttcca cccggaggtc acacacacgg ttcagggcaa ggccatgctc
     3541 gggcgtttcg tgcacgagat ttgcggctgc aagtcggact ggaacatgcc ggactacatc
     3601 agtgaggcgg tcgaggcgat ccgcaagcag gtcggcagcg atgaagtgat cctcggcctg
     3661 tccggcggcg tcgacagcag tgtggcggcc gcgctgatcc accgcgcgat cggcgaccag
     3721 ctgacctgcg tgttcgtcga ccacggcctg ctgcgcctgg acgagggcac gatggtgatg
     3781 aacatgttcg cgaagaacct cggcgtgaag gtgattcacg tcgacgcgac cgagcagttc
     3841 atgggcaagc tggccggcgt gaccgacccg gaagccaagc gcaagatcat cggccgcgag
     3901 ttcgtcgagg tgttccagga cgaggcgggc aagctgaaga atgcgaaatg gctggcccag
     3961 ggcacgattt atccggacgt gatcgaaagc gccggcaagg gcaagaagac cgcgcacacg
     4021 atcaagagcc atcacaacgt cggcggcttg ccggagacgc tcaacctgaa gctgctggag
     4081 ccgctgcgcg agctgttcaa ggacgaggtg cgcgagctgg gcgtcgcgct cgggctgccg
     4141 cacgacatgg tgtaccgcca tccgttcccc ggcccgggcc tgggcgtgcg catcctcggc
     4201 gaagtgaaga aggagtatgc ggatctgctg cgccgcgccg acgcgatctt catcgaggag
     4261 ctgcgcgcgg cgaaggacga ggaaggcaag tcctggtacg acaagaccag ccaggcgttc
     4321 gcggtgttcc tgccggtcaa gtccgtcggc gtgatgggcg acgggcgcac ctacgagtac
     4381 gtggtcgcgc tgcgcgcggt gcagacgcag gacttcatga ccgcgcactg ggcacacctg
     4441 ccgcacgatc tgctgggccg ggtgtcgaat cgcatcatca acgaggtgcg cgggctgaac
     4501 cgggtcgtct atgacatttc cggcaagccg ccggcgacga tcgagtggga atgacggcgc
//
LOCUS       DATLGF010000077         4637 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_82667,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000077 DATLGF010000000
VERSION     DATLGF010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82667"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VM406_02265"
     CDS             <1..681
                     /locus_tag="VM406_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HVL74815.1"
                     /translation="QVRRVLQRKGFDGAQIMILDALLKLRQVCCDPYLLKGVRHDPGM
                     ERAKMDMLRDMLPAMVEEGRRILMFSQFTELLGLIEPELDAMGLPWLALTGKTAPSER
                     ADLVHRFQRGQVPILLVSLKAGGVGLNLTAADTVIHLDPWWNPAVVEQATARAHRIGQ
                     TQPVFVYKLVVEGSIEERMLELQARKTALADGVLGSDPGMSEKFGPDELGRLLAPMDK
                     HGVFSGTT"
     gene            771..1295
                     /locus_tag="VM406_02270"
     CDS             771..1295
                     /locus_tag="VM406_02270"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HVL74816.1"
                     /translation="MKANDGLVLRLERFLPFPRAVVYRALTEPGELVKWWGPRGFSVP
                     AIAFNPQVGGRFRITMQPPDGDAFHLSGTFREVDPPARLAYTFQWTPPDPDDRETLAA
                     LSLEERDGGTELVLTHSGFATEQRRALHEAGWTESLDRLEQALSARHIERSFSSRLAP
                     SSERPPPGSGSGDL"
     gene            1567..2577
                     /locus_tag="VM406_02275"
     CDS             1567..2577
                     /locus_tag="VM406_02275"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVL74817.1"
                     /translation="MQQRSLGPFQVSAIGLGCMNLSHAYGAPVPEAEAERLLLRALDL
                     GITHFDTAALYGFGANETLVGKVLGKHRSRFTLASKCAMTGVRGEDGALRRVIDGRPE
                     TLKRTCEEALKRLQTDVIDLYYLHRWDKQVPIEDSMGAMADLVKEGKIRTVGLSEVSA
                     ATLRRAHAVHPVTALQTEYSLWTRNPEIAVLEACRELGVSFVAFSPVARGFLAGALTA
                     MKDVDAFDAKDIRRTMPRFTPEHFPANLRLLDGYRALARELDTTPAQLALAWLLRKGQ
                     DILPIPGTTRIAHLEENVAAVNVSLPDEAIARVEAIVNQQTVSGNRYPAQSRAEVDTE
                     EF"
     gene            2661..2906
                     /locus_tag="VM406_02280"
     CDS             2661..2906
                     /locus_tag="VM406_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HVL74818.1"
                     /translation="MFSLPPLPAAPPGRYRHYKGGEYEVLGVARHSESLEPLVLYRPL
                     YNDSGLWVRPYAMFFGDVDVNGKQVARFARVDEQDKG"
     gene            complement(3186..4154)
                     /locus_tag="VM406_02285"
     CDS             complement(3186..4154)
                     /locus_tag="VM406_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL74819.1"
                     /translation="MRKVLAGGAAILLALTVSVVVGSYLLDTRHAYERVRGKSKILAS
                     PFGNIEYTEGGKGPPVLVIHGGGGGYDQGELVVDAVLGDDFHWITPSRFGYLGSSMPA
                     SATWDEQAHAYAFLLDHLDIERVALVALSQGGPSALLLAAMYPERVSSLTCLSCGVVA
                     STSADQAQANQKGDMLRTVFAHDFTYWFISKYFKRRLMGVLGASEAVVAGLTPEERSL
                     IERIIAYMNPAAPRSAGVVLDNTAPLPGKRIASIAAPTLIVHAKDDLLQLYHNAEFAA
                     ANIPGAQLMHFETGGHVVVVVQREAIGAAIRRHIRLHADTTSLQVQ"
     gene            complement(4262..>4637)
                     /locus_tag="VM406_02290"
     CDS             complement(4262..>4637)
                     /locus_tag="VM406_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HVL74820.1"
                     /translation="ATDQRRIAPSHQSNGDGYLLTRETIAWFHAHYIQDADGDLDWRA
                     SPLLHGDLSGLPPAFVLVAGYDPLRDEGCQYAQRLSAAGNRCTLVNFERQIHGFITMG
                     RVIDEAHDAVALCALQLRHALA"
BASE COUNT          805 a         1499 c         1539 g          794 t
ORIGIN      
        1 caggtgcgcc gcgtcctgca gcgcaagggc ttcgacggcg cgcagatcat gatcctcgat
       61 gcgctgctca agttgcggca ggtttgctgc gacccctatc tgctcaaggg cgtgcggcac
      121 gaccccggca tggagcgcgc gaagatggac atgctgcgcg acatgctgcc cgccatggtg
      181 gaggagggcc ggcgcatcct aatgttctcg cagttcaccg aactgctggg cctgatcgaa
      241 cccgaactgg acgcgatggg tttgccgtgg cttgccctga ccggcaagac ggcgccgtcc
      301 gagcgcgcag atctcgttca ccggttccag cgcggccagg tgccgatcct gctggtcagc
      361 ctgaaggccg gcggcgtcgg cctcaacctc accgcagcgg acaccgtgat ccatctggac
      421 ccgtggtgga atcccgcagt cgtcgagcaa gccaccgcgc gcgcccaccg catcggccag
      481 acccagcccg tgttcgtcta caagctggtg gtggagggga gcatcgagga aagaatgctg
      541 gagctgcagg cgcgcaagac cgcgctggcg gacggcgtgc tggggagcga tccggggatg
      601 tcggaaaagt tcgggcctga tgaactgggc cggctgctcg cgccgatgga taagcacggt
      661 gtgttcagcg gcacaacatg aagcggtcgg gaatggcaat gatcttgctt gacccatgct
      721 gcgatgtgca aggcgcagcc aggcagcggg aagcacaacg gagaagtgag atgaaggcca
      781 acgatggact ggtgctgcgc ctggagcggt tcctgccctt tccccgcgcg gttgtctacc
      841 gcgcgctgac ggagccgggc gagctcgtca aatggtgggg gccgagaggc ttcagcgtgc
      901 ctgccattgc gttcaatccg caagtcggcg gccgctttcg gatcacgatg cagccaccgg
      961 atggcgacgc cttccatctt tcgggaacgt tccgggaggt cgatcctccc gcccgtctgg
     1021 catacacgtt tcaatggacc ccgcctgatc cggacgatcg cgagacgctt gccgcccttt
     1081 ccctggagga gcgagatggc ggaaccgagc ttgtcctcac gcatagcggc tttgccacgg
     1141 aacaacggcg cgccttgcat gaggcaggat ggaccgaaag tcttgatcgg ctggagcagg
     1201 cgctcagcgc acggcacatt gaacgttcgt tttcttcccg ccttgccccc agcagcgagc
     1261 gaccgccccc aggttcaggt tccggcgacc tttgaagcga acgcgccggt tccgtcggca
     1321 gaaaaagccg aatgcggatt ggaacagccg gcgggaagct atggacaagc ggacgcgagc
     1381 cggatcttca tccggtcctc gcgcggcggt gcgttcggcg gcctcacagg cacgtgtgaa
     1441 atgctggagc gactggcccg ctgcacaaaa tcaccattcg tcgtaatctc cctgcaggtt
     1501 ctatcgccgt ctcggcggct ccgttacctt cgaccggagc cgcccttttt tttcaggagc
     1561 gaccccatgc aacaacgatc cctcggcccc ttccaggtca gcgccatcgg tctgggctgc
     1621 atgaacctga gccacgccta cggcgcgccg gtccctgaag cggaagcgga acgcctgctg
     1681 ctgcgcgcgc tcgatctcgg catcacccat ttcgacacgg ccgccctgta cggcttcggc
     1741 gccaatgaaa ccctggtcgg caaagtgctc ggcaagcatc gctcgcgctt caccctggct
     1801 tccaagtgcg cgatgaccgg cgtgcgcggc gaggatggcg cgctgcgccg tgtgatcgac
     1861 gggcgtccgg aaacgctcaa gcgtacctgc gaggaagccc tcaagcgcct gcagacggac
     1921 gtgatcgatc tctactacct gcaccgctgg gacaagcagg tgccgatcga ggacagcatg
     1981 ggcgcgatgg cggatctggt gaaggagggc aagatccgca cagtcggcct gtccgaagtg
     2041 tcggccgcga cgctgcgccg tgcgcatgcc gtgcatccgg tgaccgcgct gcagaccgag
     2101 tattcgctgt ggacgcgcaa tcccgagatc gccgtgctgg aggcctgccg cgaactcggc
     2161 gtcagctttg tggccttcag tccggtcgcg cgcgggtttc tcgccggagc attgaccgcc
     2221 atgaaggatg tcgacgcctt cgacgcaaag gacatccgcc gcacgatgcc gcgcttcacc
     2281 cctgagcatt tccccgccaa cctgcgcctg cttgacggct accgtgcgtt ggcccgcgaa
     2341 ctggacacca cgccggccca gctggccctg gcctggctgc tgcgcaaggg gcaggacatt
     2401 ctcccgatcc ccggcacgac ccgtatcgcg catctcgaag agaacgtcgc ggcagtgaat
     2461 gtgagcttgc cagacgaggc cattgcacgg gtggaggcga tcgtgaacca gcagacggtg
     2521 agcggcaatc gttatccggc gcaatcgcgg gccgaggtgg atacggagga gttttgagca
     2581 tgcgggcgca ggctgcccgg tgcagcgccc cgcgcaactt gctaccatgg ccgcgtccga
     2641 tctcgctccg gttcaaccac atgttcagcc tgcctccgct acccgctgca ccgcctggcc
     2701 gctatcgcca ctacaagggc ggcgagtatg aagttctcgg cgtcgcgcga cacagcgaaa
     2761 gcctggaacc tctcgtgctg taccggccgc tgtacaacga cagcgggctg tgggtgcgtc
     2821 cgtacgcgat gttcttcggc gacgtggacg tgaacgggaa gcaggtggca cgttttgccc
     2881 gcgtcgatga acaggacaag ggctgagggc ccgctgactt tgaagggagg cgaggatcgt
     2941 ggtccgtatc gtcgggctgc gcagcgaccg cgtgcccgac gaaggtctgc gcatcggcac
     3001 ggtgcgcaga ccgcgcaacg acgctgggtc ccggctttcg ccgggacgac ggcccggcgg
     3061 cgtgcagctg cggcttcact ccggcacgca gcgcggtagg ctgggatgaa atcccagctt
     3121 tcgattccga ttggatcctt gctgggattt catcccagcc tacggccctg ccggccggcg
     3181 gtcgatcatt gcacctgcag cgaggtcgtg tccgcgtgca aacggatgtg tctgcgaatc
     3241 gccgcgccga tcgcctcgcg ctgcacgacc accacgacat ggccgcccgt ttcgaagtgc
     3301 atcagctgcg ctccgggaat gttggcggcg gcgaactcgg cgttgtggta gagctgcagc
     3361 aggtcgtcct tcgcatgcac gatcaaggtc ggcgcggcga tcgaggcgat cctcttcccc
     3421 ggaagaggcg cggtattgtc gagaacgacc cctgccgagc ggggtgccgc cggattcatg
     3481 tacgcgatga tgcgctcgat gagcgatcgt tcctcgggcg tcaaccctgc caccacggcc
     3541 tcgctggctc cgagcacgcc catgagccgt cgcttgaagt acttcgatat gaaccagtac
     3601 gtgaaatcgt gggcgaacac ggtcctcagc atgtcgccct tctggttcgc ctgcgcctgg
     3661 tctgccgagg tcgaggccac gacgccgcag gacaggcagg tcagcgagga cactctctcg
     3721 ggatacattg ccgccagcag gagcgcggag gggccgccct gagagagggc gacgagcgcg
     3781 actcgctcga tgtcgaggtg gtcgagcaga aaggcgtacg cgtgcgcctg ctcgtcccag
     3841 gtggcactcg ccggcatgct ggaaccgaga tagccgaacc gtgacggggt gatccagtgg
     3901 aaatcgtccc caagcaccgc atccaccacg agctcgccct ggtcgtagcc gcccccgccg
     3961 ccatgaatga cgaggaccgg ggggcccttt cctccctcgg tgtactcgat gtttccgaag
     4021 ggcgaggcca ggatcttgct tttgccacgc acacgctcat acgcgtggcg ggtgtcgagg
     4081 agatagctcc cgacgaccac ggacaccgtc agggcaagca gaattgccgc cccgccggcg
     4141 aggacctttc gcatcatcgc ctccactggc aacggggtat cgacactcca gtttagtgcc
     4201 gtatggcggg atttcgtccc aggctccggc cctgcgctcc cgatgcgaac acgctccgcg
     4261 atcaggcgag cgcgtggcgc agctgcaacg cgcacaaggc caccgcatcg tgggcttcgt
     4321 cgatcacccg ccccatcgta atgaagccgt ggatctggcg ctcgaaattg accagcgtgc
     4381 agcggttgcc ggccgccgaa aggcgctgcg cgtactgaca gccttcgtcg cgcagcgggt
     4441 cgtagccggc cacgagtacg aaggccggtg gcaggccgga gagatccccg tgcagcaagg
     4501 gcgaggcgcg ccaatcgagg tcgccgtccg catcctggat gtaatgggca tgaaaccacg
     4561 cgatcgtctc gcgcgtgagc agatagccgt cgccgttgct ctggtgggat ggtgcaatcc
     4621 ggcgctggtc ggtggcg
//
LOCUS       DATLGF010000078         2431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_20012,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000078 DATLGF010000000
VERSION     DATLGF010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20012"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VM406_02295"
     CDS             <1..470
                     /locus_tag="VM406_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HVL74821.1"
                     /translation="PAAVQTTFACTEVGNFSNDEAQRFAAVLGDLAQRAQRRPVQAAS
                     SYMVYMPPQPDREGAERKAAELRSLGIHDFFIIQDNSALRWGISLGVFKTEEGARTHL
                     AGLSQKGVRSARIGERGAGAALVAFQFADLDEGARATLEQATAGMRQTVKDCP"
     gene            complement(467..1582)
                     /locus_tag="VM406_02300"
     CDS             complement(467..1582)
                     /locus_tag="VM406_02300"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HVL74822.1"
                     /translation="MMRIAGAVAALVVLSACSREVTVKIGVVAPMSGALAQYGKDIAN
                     GARVAVDELNNEHFMIKGKRARFELVVEDDKASPEEGKAAAQRLIDAKVAAVFGHFNS
                     GVSIAAAPAYAQAGIPQLSVSTNPQYTRMGLKTTFRITADDIQQGATLGRLISERLRS
                     RNLYVVDDKTLFGIGVADEVERAVKNRRIDPLRESVDGKSADYASLAQKIIAANPDAV
                     FFGGDEAVGLPLLKALRAAGSSARFVVADAMCDNSTIKGAGGAANANYYCSIAGVPPS
                     WLSAGIGFMEMYKKQYGTPGAYSALSYDGIHVLAQAMQEAGSAEPSDYLPVLAKGSFS
                     GKIQGAIEFDAKGDIKDGTVVIYQSVGGQLVEQRNLL"
     gene            complement(1727..1978)
                     /locus_tag="VM406_02305"
     CDS             complement(1727..1978)
                     /locus_tag="VM406_02305"
                     /inference="COORDINATES: protein motif:HMM:NF025733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HVL74823.1"
                     /translation="MPAHLSRLRPAPMSTCQRCKTAFTCGMADTKDSMQPCWCTALPA
                     LAPDLYKDAVGCYCPACLRELVSRQSAPSPSSASVPPKL"
     gene            2065..>2431
                     /gene="argB"
                     /locus_tag="VM406_02310"
     CDS             2065..>2431
                     /gene="argB"
                     /locus_tag="VM406_02310"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HVL74824.1"
                     /translation="MSDFPSSAADLSAVSPQIKAQILAEALPYIRKFHGKTIVVKYGG
                     NAMTEERLKHGFARDVILLKLVGMNPVVVHGGGPQIDNALKRLGKQGTFVQGMRITDE
                     ETMEVVEWVLGGEVQQDIVM"
BASE COUNT          425 a          769 c          805 g          432 t
ORIGIN      
        1 ggccggccgc cgtccagacg accttcgcct gcaccgaggt gggcaatttc agcaacgacg
       61 aggcgcagcg cttcgccgcc gtcctgggcg atctcgccca gcgcgcgcag cgccggccgg
      121 tgcaggcagc cagcagctac atggtctaca tgccgccgca gccggaccgt gaaggcgcgg
      181 aacgcaaggc cgccgagctg cgcagcctgg ggatccacga cttcttcatc atccaggaca
      241 actccgcgct gcgctggggc atatcgctgg gcgtcttcaa gaccgaggag ggcgcgcgca
      301 cccatctggc gggcctgagc cagaagggcg tgcgcagcgc gcgcatcggc gagcgtggcg
      361 cgggagcggc gctggtcgcc ttccagttcg ccgatctgga cgagggcgcg cgcgccacgc
      421 ttgaacaggc gaccgccggc atgcggcaga cggtgaagga ctgcccttaa agcaggttgc
      481 gctgctccac cagctgtccg ccgacggact ggtagatcac cacggtgccg tccttgatgt
      541 cgcccttcgc atcgaactcg atcgcgccct ggatcttgcc ggagaacgaa cccttggcca
      601 gcaccggcag atagtcggac ggctcggccg atcccgcttc ctgcatcgcc tgggcgagca
      661 catggatgcc gtcatacgag agggcggagt acgctcccgg cgttccgtac tgcttcttgt
      721 acatctccat gaagccgatg cccgcggaca gccacgaggg cgggacgcct gcgatcgagc
      781 agtagtagtt cgcgttggcc gcgccaccgg cgcccttgat ggtggagttg tcgcacatcg
      841 cgtcggcaac cacgaagcgc gcggacgaac cagccgcgcg cagggccttc aacaagggca
      901 ggccgaccgc ctcgtcgcca ccgaaaaaga cggcatccgg attggcggcg atgatctttt
      961 gcgcgaggct ggcgtagtcg gcgctcttgc cgtcgacgct ctcgcgcagc ggatcgatcc
     1021 tgcgattctt caccgcgcgt tcgacctcgt cggccacgcc gatgccgaac agcgtcttgt
     1081 cgtcgacgac atacaggttg cggctgcgca ggcgctcgct gatcaggcgg ccgagggtcg
     1141 cgccctgctg gatgtcgtcc gccgtgatgc ggaaggttgt cttcagcccc atgcgcgtgt
     1201 actgcggatt ggtcgagacc gacagctgcg ggatgccggc ttgcgcgtac gcgggagcgg
     1261 ccgcaatcga cacgccggag ttgaagtgtc cgaacaccgc agcgaccttc gcgtcgatca
     1321 ggcgctgggc ggccgccttg ccttcctcgg ggctggcctt gtcgtcttcc acgaccagtt
     1381 cgaagcgcgc gcgcttgccc ttgatcatga agtgctcgtt attgagttca tcgaccgcca
     1441 cgcgcgcgcc gttggcgatg tccttcccgt attgcgccaa ggcccccgac atcggcgcaa
     1501 ccaccccgat cttgacggtc acctcgcggc tgcaggcact gagcacgaca agcgccgcga
     1561 cggcgccggc tatacgcatc attcccatgt ctcctccttg ttattggtgt caacgaacgc
     1621 cgattcatct taggcgccgt tgcacacaat taaattcgtg attgggacgc gcggcaagtt
     1681 tttatttgct atgtggtaaa aaatgctacc aatggttaca gaatgcttac agcttgggcg
     1741 ggacggacgc agaggagggg gaaggggcgc tctggcggct gaccagttcg cgcaggcagg
     1801 cggggcagta gcagccaact gcgtccttgt acaggtcggg ggcaagcgcg ggcagggccg
     1861 tgcaccagca gggctgcatg gaatccttgg tatcagccat gccgcaggtg aacgcggttt
     1921 tgcaacgttg gcaggtgctc atgggggccg ggcgaagtct ggacaggtgt gctggcattt
     1981 tacttgcgtg aatgcgctgc cgcagtgcag tgctcccgct gcattcgctg taaaatcccg
     2041 gcacttttct ccacagggca ccccatgagc gatttcccca gttcggccgc cgacctgagc
     2101 gcggtctccc cgcagatcaa ggcgcagatc ctggccgagg cgctgcctta catccgcaag
     2161 ttccatggca agaccatcgt cgtcaaatac ggcgggaatg cgatgaccga agagcgcctg
     2221 aagcacggat tcgcgcgcga cgtgatcctg ttgaagctgg tcggcatgaa tccggtggtc
     2281 gtgcatggcg gcggtccgca gatcgacaac gcattgaagc ggctcggcaa gcagggcacc
     2341 ttcgtgcagg gcatgcgcat caccgatgaa gagacgatgg aagtcgtcga atgggtgctg
     2401 ggcggcgagg tgcagcagga catcgtcatg c
//
LOCUS       DATLGF010000079         2468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_130559, whole genome shotgun sequence.
ACCESSION   DATLGF010000079 DATLGF010000000
VERSION     DATLGF010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130559"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /gene="folP"
                     /locus_tag="VM406_02315"
     CDS             complement(<1..788)
                     /gene="folP"
                     /locus_tag="VM406_02315"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HVL74825.1"
                     /translation="MKNSLHCGRFQLPLDGEGVLPLVMGILNVTPDSFSDGGRFASLD
                     AALYAAEQMIADGVDIIDIGGESSRPGAQPLSLDQELERVMPLVYALRDCGKPLSVDT
                     YKPDVMCEVLGAGVDMINDINGFRAEGALNAVKDSDCALCIMHMLGSPATMQIEPCYG
                     DVVAEVEAFLLERIAQCEAAGIARQRLCIDPGFGFGKTVVHNLALLKRLDRLAGHGIP
                     VLAGLSRKSTIGALTGRPVEQRLAGSLAAVLAAVSHGARIVRVH"
     gene            complement(854..>2468)
                     /gene="ftsH"
                     /locus_tag="VM406_02320"
     CDS             complement(854..>2468)
                     /gene="ftsH"
                     /locus_tag="VM406_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HVL74826.1"
                     /translation="VKFDVKQPEEQSFLSQVFISWFPMLLLIGVWIFFMRQMQGGGKG
                     GAFSFGRSKARMLDEASNNVTFADVAGCDEAKEEVQELVDFLRDPTKFQKLGGRIPRG
                     VLMVGPPGTGKTLLARAIAGEAKVPFFTISGSDFVEMFVGVGASRVRDMFEQAKKHSP
                     CIIFIDEIDAVGRHRGAGMGGGNDEREQTLNQLLVEMDGFEANSGVIVIAATNRADVL
                     DKALLRPGRFDRQVMVGLPDIRGREQILYVHMRKVPIAPDVKADILARGTPGFSGADL
                     ANLVNEAALFAARRSKRLVEMQDFEDAKDKIVMGPERKSAVMREEERRNTAYHESGHA
                     VVAKLLPKADPVHKVTIMPRGYALGLTWQLPEHDRVNMYKDKMLEEIAILFGGRIAEE
                     IFMGQMSTGASNDFERATKLARAMVTRYGMSENLGTMVYEDSEQDAYFGRMSAKTVSE
                     ATQQKVDAEIRAILDQQYALARHLLEEHREKVDVMAKALLEWETLDADQVNDIMEGKD
                     PRPPKTGAPAKKPSADGASGDISPNPAPA"
BASE COUNT          437 a          819 c          760 g          452 t
ORIGIN      
        1 tcgtgtacgc gcacgatgcg agcgccatgg gaaacggcgg cgagcaccgc ggccaggctt
       61 ccggccaggc gctgctcgac cggccggccg gtaagcgcac cgatcgtcga cttgcgcgag
      121 agcccggcga gcacggggat gccatgcccc gcaagccgat ccagtcgctt gagcaaggcg
      181 agattatgca cgaccgtctt gccgaatccg aacccggggt cgatgcacag gcgctggcgc
      241 gcgatgccgg cagcttcgca ctgcgcgatc cgctccagca gaaacgcctc cacttcggcg
      301 acgacatcgc catagcacgg ctcgatctgc atcgtcgcgg gcgagccgag catgtgcatg
      361 atacaaagcg cgcagtcgga gtccttgacc gcgttcaacg ccccctccgc gcgaaatccg
      421 ttgatgtcat tgatcatgtc cacgccggcg cccagcacct cgcacatcac gtccggtttg
      481 taagtgtcaa cggaaagcgg cttgccgcaa tcgcgcagcg cgtagaccag gggcattaca
      541 cgctcgagtt cctgatcaag agacaagggt tgcgctccgg gccgactgga ctccccgcct
      601 atgtcgatga tgtccacgcc gtcagcaatc atctgctctg ccgcatacag cgcagcatcc
      661 agggacgcga accggccgcc gtccgagaat gaatcgggag tcacgttcag tatccccatc
      721 accaggggca gcaccccttc cccgtcgagc ggcagctgaa agcgtccgca gtgcaagctg
      781 ttcttcatgg tgtcgtgaca aacaaaaagg gcgaggattt ctcctcaccc ttgtcgatgg
      841 attgcggccg tgctcaggcc ggtgcgggat tcggcgaaat gtcgccggaa gcgccatcgg
      901 cggacggctt cttcgcgggc gcgcccgtct tcggaggacg cggatccttg ccctccatga
      961 tgtcgttgac ctggtcggcg tccagcgtct cccattcgag cagggccttg gccatcacgt
     1021 cgaccttctc gcggtgctct tcaagcagat ggcgcgccag cgcatattgc tgatcgagga
     1081 tcgcgcggat ctccgcgtca accttttgct gggtcgcctc ggacaccgtc ttggccgaca
     1141 tgcggccgaa gtacgcatcc tgctcgctgt cctcataaac catggtgccg aggttctcgg
     1201 acatgccgta gcgggtcacc atcgcacgcg cgagcttggt cgcccgctcg aagtcgttcg
     1261 aggcaccggt cgacatctgc cccatgaaga tctcttcggc aatgcgtccg ccgaacagga
     1321 tcgcgatctc ctcgagcatc ttgtccttgt acatgttcac gcggtcgtgc tcgggcagct
     1381 gccaggtcag gcccagcgca tagccgcgcg gcatgatcgt gaccttgtgc accggatcgg
     1441 ccttcggcag cagcttggcg accaccgcat ggccggactc gtggtaggcc gtgttgcggc
     1501 gctcttcctc gcgcatcacc gcggacttgc gctccggacc catcacgatc ttgtccttcg
     1561 cgtcctcgaa atcctgcatc tcgacgaggc gcttgctgcg gcgggcggcg aacagcgcgg
     1621 cttcgttaac caggtttgcg aggtcggcgc cggagaagcc gggggtgccg cgggcgagga
     1681 tgtcggcctt cacatcgggt gcgatcggca cctttcgcat atgcacatac aggatctgct
     1741 cgcggccgcg gatgtcgggc aggccgacca tcacctggcg atcgaagcgg cccggacgca
     1801 acagcgcctt gtcgagcacg tcggcccggt tggtcgcggc gataacgatg acgccggaat
     1861 tggcttcgaa gccgtccatc tcgaccagca gctggttgag cgtctgctca cgctcgtcgt
     1921 tgccaccgcc catgccggcg ccgcgatggc gcccgaccgc gtcgatttca tcgatgaaga
     1981 tgatgcacgg agaatgcttc tttgcctgct cgaacatgtc gcgcacgcgc gaggcaccca
     2041 cgccaacgaa catttcgacg aagtcggagc cggaaatcgt gaagaacggc accttcgcct
     2101 caccggcgat cgcgcgggcg agcagcgtct tgccggtgcc gggcgggccg accatcagca
     2161 cgcctcgggg aatgcggccg cccagctttt ggaacttggt cggatcgcgc aggaagtcga
     2221 ccagctcctg gacttcctcc ttcgcttcgt cgcagccggc gacgtcagca aacgtgacgt
     2281 tgttgctggc ctcgtcgagc atgcgcgcct tcgaacggcc gaatgaaaag gctccgccct
     2341 tgccgccgcc ctgcatctgg cgcatgaaga agatccacac gccgatcaac agcagcatcg
     2401 ggaaccatga aatgaatact tgcgacagga agctctgctc ttccggttgc ttgacgtcga
     2461 acttgacc
//
LOCUS       DATLGF010000080         5336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_191805, whole genome shotgun sequence.
ACCESSION   DATLGF010000080 DATLGF010000000
VERSION     DATLGF010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191805"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /locus_tag="VM406_02325"
     CDS             complement(<1..1021)
                     /locus_tag="VM406_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HVL74827.1"
                     /translation="MLPLSPSIFKAYDIRGIIGKTLDIGIARKIGQAFGTAARIRDER
                     TVVIGRDGRLSGPDLSAALASGLQRAGVDVIDLGVVATPLVYFATQVLDGEHSAQSGI
                     MVTGSHNPPDYNGFKMVLAGEAIYGDAIQSLYRAIEKGDFAQGNGSYSTYDIRAAYLE
                     RIASDVKLARPMKIAVDCGNGVAGAFAGDLYRALGCEVIELFCDVDGNFPNHHPDPAH
                     PENLQDLIRCLRETDAEIGLAFDGDGDRLGVVTKDGQIIYPDRQLMLFAADVLTRHPG
                     REILYDVKCTRHIAPWVRKHGGVPLMWKTGHSLVKAKMRETGAPLGGEMSGHIFFKDR
                     WYGFDD"
     gene            complement(1124..3382)
                     /locus_tag="VM406_02330"
     CDS             complement(1124..3382)
                     /locus_tag="VM406_02330"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HVL74828.1"
                     /translation="MMNQSIPRLPGPTAARPVQKEAELASYFDTVWDNRWLILGIAFI
                     VALAGVVYAMLAKPVYQATMLIHVEEEATRQPANIITDLNSMFDVKATASAEMELLQS
                     RLVVARAIDNLRLYISARPKQFPIVGGWIAERNRGLSEPGLFGYGGYTWGAEKINVGV
                     FNVPDAMHNREFTLVAQPDGAYRLLQQDSGIDVQARVGETAIFELPRGTIEIRVDQLE
                     ARPGAHFQLVRSSRLAMIEGVQRSLRVGEQGGRQSGVIGVQLQGGDPSVIHAILTEVG
                     KEYVRQNTARKTEDAEKTLAYLNKQLPELKTQLEQAEAKYNAYRNQHGTIDLGEESRL
                     SLQQSSAAKLRLMELQQRRIELLARYTTDHPAVQGVDSQLRELRSQVNSLESHIKTLP
                     NVEQNLLRLRREVDVNTELYTALLNTQQQLRLASIGRVSNVRLVDAPMLPERPIKPNR
                     PVIIALAILGGLFLGILAAFVRKSLSASIDTPEQLEDVAGVPVYATIPHSRKQRELYQ
                     QVNVKHQKVPLLANVSSSDIAVESLRNFRTALQFALPSFKNNIVLISGATPGMGKSFV
                     SANLAAIMAATGKRVLLIDGDLRNGLLHLYFGFGRHDGLSDAIAGARRLDQVIHRSVI
                     ENMDFITTGTLPQHPAELLLRPNFGAILQELSGHYDLVLIDAPPILAVADTMIIGAHA
                     GGIYLMTRAGVTTPGEVQESIKRLRQAGLEPNGVLLNDLKPRPGRYGYGYTYGVPYGQ
                     RPAGSPPLIEAS"
     gene            complement(3521..4699)
                     /locus_tag="VM406_02335"
     CDS             complement(3521..4699)
                     /locus_tag="VM406_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011004310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HVL74829.1"
                     /translation="MRNSATAFKMYGVAALLPLLAACGTVAPGLHFDRGSAASGASAP
                     STEQAAGAETDAMKAVLKPITPQLVRAEAEARERQATQDISALLGKPESYKIGPGDVL
                     SIVVWDHPELSNAATVATGGPIGPGADAATSAAAAPPAGFIVDHDGVIQFPYAGEVKV
                     SGLTQDQARNVVTQKLARYIRDPRVTLRVQSFRSKRVYVDGEVRSPGLQAINDIPMTL
                     VEAINRAGGVLPTGDQSQIVVSRGGTSYHINLPQLVQRGVNPTSIMLANGDVVRVRSR
                     EESKVFVSGEVVSPRALPMHNGRLSLNEALGEAGGVNPLSGDGSQIYVVRRNGAEQVV
                     YQLDGRSQGALAMAEGFELAPKDVVYVAATPLANWHRAISLILPGALSSAVGAVGPAR
                     "
     gene            complement(4970..>5336)
                     /locus_tag="VM406_02340"
     CDS             complement(4970..>5336)
                     /locus_tag="VM406_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HVL74830.1"
                     /translation="LFLFTLASGVLVLYAALVGTQDERIRESGLLRALGATRRQLTRA
                     QWIEFALVGGLAGMLAATGAALVGWVLAGRVFNFDWTFSPVVWIAGLVVGALCAFIGG
                     WFGLRNVLRHPPLHTLRGA"
BASE COUNT          899 a         1738 c         1783 g          916 t
ORIGIN      
        1 cgtcgtcgaa gccgtaccag cggtccttga agaagatgtg gccgctcatc tcgccgccca
       61 gcggcgcacc ggtctcgcgc atcttggcct tgaccagcga gtggcccgtc ttccacatca
      121 gcggcacgcc gccgtgcttc ctgacccatg gtgcgatgtg ccgcgtgcac ttgacgtcgt
      181 acaggatctc gcgccccgga tggcgcgtga gcacgtcggc cgcgaacagc atcagctggc
      241 ggtccggata gatgatctgg ccgtccttgg tcaccacgcc gaggcggtcg ccgtcgccgt
      301 cgaaggccag gccgatttcg gcatcggtct cgcgcaggca ccggatcagg tcctgcaggt
      361 tctcaggatg ggcgggatcg ggatggtggt tcgggaagtt gccgtctacg tcgcagaaca
      421 gttcaatgac ttcgcagccg agcgcgcggt agaggtcgcc ggcgaaagcg ccggccacgc
      481 cgttgccgca atccacggcg atcttcatcg ggcgcgcaag cttcacgtcg gatgcgatgc
      541 gctcgaggta ggctgcgcgg atgtcatagg tggaatagct gccgttgccc tgcgcgaagt
      601 cgcccttctc gatcgcgcgg tacaggctct ggatcgcgtc gccgtagatc gcctcgccgg
      661 cgagcaccat cttgaaaccg ttgtagtccg gcggattgtg gctgccggtg accatgatgc
      721 cggactgcgc cgaatgttca ccatcgagca cctgggtggc gaaatacacg agcggcgtgg
      781 cgaccacgcc aagatcgatc acgtccacgc ctgcacgttg caggccggag gccagcgccg
      841 cggacagatc cgggcccgac aggcgcccgt cgcgcccgat caccacggtg cgctcgtcgc
      901 ggatgcgcgc ggcggtgccg aacgcctggc cgatcttgcg ggcgatgccg atgtccagag
      961 tcttgccgat gatgcctcgg atgtcgtagg ccttgaaaat ggagggggaa agtggaagca
     1021 cgatgttctc agactgaaga tggaggagag gtccgccttc ctcctggcag gaggaagcat
     1081 gcaagcggcg agacgcgaat gcgtctcgcc ggggccgacg cgtttacgac gcctcgatca
     1141 gcggcgggct tcccgcgggg cgctgaccgt aaggcacccc gtaagtatag ccatacccat
     1201 aacggcccgg gcgcggcttg agatcgttga gcagcacgcc gttgggctcc agccccgcct
     1261 ggcgcagccg cttgatcgac tcctgcactt cgcccggcgt ggtcacgccg gcgcgcgtca
     1321 tcaggtagat gccgcccgca tgcgcgccga tgatcatcgt gtcggcaacg gccaggatcg
     1381 gcggcgcgtc gatcagcacc aggtcatagt ggcccgacag ttcctgcagg atcgcgccga
     1441 aattcgggcg cagcagcagt tcggccggat gctgcggcaa cgtgccggtc gtgatgaagt
     1501 ccatgttctc gatcacgctg cggtggatca cctgatcgag gcggcgtgcg cccgcgatgg
     1561 cgtcggagag accgtcatgg cgtccgaaac cgaagtacag gtgcaacagg ccgttgcgca
     1621 gatcgccgtc gatcagcagc acgcgcttgc ccgttgcggc catgattgcg gcgaggttcg
     1681 ccgacacgaa ggacttgccc atgcccggtg tcgcgccgga gatcagcacg atgttgttct
     1741 tgaagctggg cagcgcgaac tgcaacgccg tgcggaagtt gcgcaggctc tcgaccgcga
     1801 tgtcggatga cgacacattt gccagcagcg gcaccttctg gtgcttgacg ttgacctgct
     1861 ggtagagctc gcgctgcttg cggctgtgcg gaatggttgc gtacacgggc acgccggcca
     1921 cgtcttcgag ctgctcggga gtatcgatgc tcgcgctcag cgacttgcgc acgaaagcgg
     1981 ccaggatgcc aaggaacagg ccgccaagta tcgccaacgc gatgatgacc ggccggttcg
     2041 gcttgatcgg ccgctccggc agcatcggcg cgtccaccag gcgcacgttg gagacgcgtc
     2101 cgatcgatgc cagcctgagc tgctgctggg tgttcagcaa cgcggtgtac agctcggtgt
     2161 tcacatccac ctcgcggcgc aggcgcagca ggttctgctc gacattcggc agggtcttga
     2221 tgtgcgactc cagggaattg acctgcgagc gcagttcgcg cagctggctg tcgacgccct
     2281 gcacggccgg atgatcggtg gtgtagcgcg ccagcagttc aatgcggcgc tgctgcaact
     2341 ccatcaggcg cagcttcgcc gccgacgact gctgcagcga caggcgcgac tcttcgccaa
     2401 gatcgatcgt gccgtgctgg ttgcggtagg cgttgtactt ggcctcggcc tgctcgagct
     2461 gcgtcttcag ttcagggagc tgcttgttca ggtaggccag cgtcttttcc gcatcttcgg
     2521 tcttgcgggc ggtgttctgc ctgacgtatt ccttgcccac ttccgtgaga atcgcgtgga
     2581 tgacgcttgg atcgccgccc tgcagctgca cgccgatcac gccggactgg cgaccgccct
     2641 gctcgcccac ccgcagcgag cgctgcaccc cttcgatcat ggcaaggcgc gaggagcgca
     2701 cgagctggaa atgcgcgccc gggcgggctt ccagctggtc gactcggatc tcgatggtgc
     2761 cccgcggcag ttcgaaaatc gccgtctcgc cgaccctcgc ctgcacatcg atgccgctat
     2821 cctgctgcag caaccggtac gccccgtcgg gctgtgccac cagcgtgaac tcccggttgt
     2881 gcatcgcgtc gggtacattg aacacgccga cattgatctt ttccgcaccc caggtatagc
     2941 cgccgtagcc gaacagcccc ggctcggaca gaccgcgatt gcgctcggcg atccagccgc
     3001 cgacgatcgg gaactgcttt ggcctggcgg agatgtaaag tcgcagattg tcgatcgcgc
     3061 gcgccaccac gaggcgcgac tgcagcagtt ccatctcggc gctggcagtg gccttgacgt
     3121 cgaacatcga gttgaggtcg gtgatgatgt tggccggctg ccgcgtcgct tcttcctcga
     3181 cgtggatcag catcgtcgcc tggtacaccg gcttggccag catcgcatac acgacgccgg
     3241 cgagtgcgac gatgaaggcg atgccgagaa tgagccagcg gttatcccaa acagtatcga
     3301 agtagctggc cagctcggcc tccttctgca ccggacgtgc agcagtgggg ccaggcagtc
     3361 tgggaatgga ttgattcatc ataatcgtct gattggttgg attcttgaac gtcggccttg
     3421 gccgatggcg gtgttcggag cggcttcggc gcaggatgca aggaagaaat gcgacggccc
     3481 ggcgcggtct cctgcgacgc gccgagccgc tgtgtcaaca tcagcgggcg ggaccgaccg
     3541 cgcccacggc cgaggacagg gcccccggca ggatcaggct gatggcgcgg tgccagttgg
     3601 ccagaggcgt ggccgcgacg tacaccacat ccttcggcgc cagctcgaag ccttcggcca
     3661 tcgccagcgc gccctgcgac cgtccgtcga gctggtagac gacctgctcg gcgccgttac
     3721 ggcgcaccac gtagatctga ctgccgtcgc cggagagcgg attgacgccg ccggcctcgc
     3781 ccagcgcctc gttcagcgag agacggccgt tgtgcatcgg cagcgcgcgc ggactgacca
     3841 cctcgccgga cacgaatacc ttgctttcct cgcgcgagcg cacgcgcacc acgtcgccgt
     3901 tggcgagcat gatcgatgtc gggttgacgc cgcgctgcac cagctgcggc aggttgatgt
     3961 ggtagctggt gccgccgcgg ctgacgacaa tctggctctg gtcgccggta ggcagtacgc
     4021 cgccggcgcg gttgatcgcc tcaaccaggg tcatcggaat gtcgttgatc gcctgcaggc
     4081 cgggactgcg cacctcgccg tcaacgtaga cacgcttgct gcggaacgac tgcacgcgca
     4141 gcgtcacgcg cgggtcgcgg atgtagcgcg ccagcttctg cgtgaccaca ttgcgcgcct
     4201 ggtcctgggt cagccccgac accttgactt cgccggcgta cggaaactgg atcacgccgt
     4261 catggtcgac gatgaagccc gccgggggcg cggccgcggc cgaggtggcg gcatccgccc
     4321 ccgggccgat cgggccgccg gtcgcgaccg tggcggcgtt ggagagttcg gggtgatccc
     4381 acacgacgat ggacagcacg tcacccgggc cgatcttgta cgactcgggc ttgcccagca
     4441 aggcgctgat atcctgggtc gcctggcgct cgcgcgcctc cgcttcggca cgcacgagct
     4501 gcggcgtgat cggcttgagc acggccttca tggcatcggt ctccgcgccg gctgcctgtt
     4561 ctgtgctcgg ggcgctggcg cctgaagctg ccgagccgcg gtcgaagtgg agcccgggcg
     4621 caaccgtgcc gcaggccgcc agtagcggca gcagcgcggc gacgccatac atcttgaaag
     4681 ctgttgctga gttgcgcata tcgaataccc caggtgatga gagcggccat cctccgatgg
     4741 cccgcgttga acgtcctgat ataccggctt gccggccggc gacttgtctc atgcacaact
     4801 ctacggacgc gggccatccc cgctttgagg ggcatcaacg ggctcctaga tatgaatcag
     4861 cgcaatatcg cgctacggaa acgaaccgtt acacgcttgt ggacacacaa aaaaagaacg
     4921 caggaacctt gcggaacctg cgttcgaatg cgcgagcgtg caagagggtt caggcgcccc
     4981 gcaaggtgtg cagcggcggg tggcgcagca cgttgcgcag gccgaaccag ccgccgatga
     5041 atgcgcacag tgcgccgacg acgagcccgg cgatccagac caccggactg aacgtccagt
     5101 cgaaattgaa cacgcggcca gccagtaccc agccgaccag ggccgcgccg gttgcggcaa
     5161 gcataccggc caggccgccg accagcgcga actcgatcca ttgcgcgcgc gtaagctggc
     5221 gccgcgtcgc gccgagcgcg cgcagcaggc ccgattcgcg aatgcgctcg tcctgcgtgc
     5281 cgaccagcgc cgcgtacagc accagcacgc ccgaggccag cgtaaacagg aacagg
//
LOCUS       DATLGF010000081         5829 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_1863,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000081 DATLGF010000000
VERSION     DATLGF010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5829
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1863"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VM406_02345"
     CDS             complement(<1..298)
                     /locus_tag="VM406_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74831.1"
                     /translation="MGRSGLFWPGLPEIDSPTLASPLLAQLADSRLEAAAASGTLVVL
                     YPDLGEPFRSVFSQMIEGIESASRLQVRSIAVAAGMTPNDVGVIVRRAGARAVFA"
     gene            complement(455..2458)
                     /locus_tag="VM406_02350"
     CDS             complement(455..2458)
                     /locus_tag="VM406_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVL74832.1"
                     /translation="MSDEDDLLLMYGDKSYVSIATGGRMPTVRAPSVATVITAEDIKA
                     IGATDLDEVLETVPGVHVSRAVITNAPVYVFRGMHHENNPQVLILLNGVPMNRLFKGN
                     RGDVWGGFPVEHIARIEVIRGPGSALYGADAFAGVINIITKNARDIDGTQTGLRAGSF
                     KTGDAWLLHGGQAGPVEIAGYLRLGTTDGAGRLIAADAQTGWDRAFNTDASLAPGRMN
                     DNRNAIDAHLDLRLRNWRWWTALKERRDVGSGVGVAQALDPTGRSYSQNLTTSLGYHE
                     RGIVPDWDLDVQASFTRYREFSDLVLYPAGAFEGRFVDGFIGNPYKWERQGRLSASAF
                     YNGFERHRVRVGAGYTAESIYRTREWRNYTPDFMPIGSGSRADVIEVTESAPFLRPQS
                     RNIRYVYLQDEWDFAADWSLTAGVRHDRYSDFGSTTNPRAALVWHAAYNLTAKLLYGS
                     AFRAPSFTELYAINNPVGRGNPGVQPERMRTIEAVLNWQARPDLQLGASVYQYRMSDM
                     LRLVGIDFANAGSQTGRGLEIEATWDLARTLRVSGNAAYQEAFDPMTGANAGNAPRTK
                     LYLRSDWRFMPGWLLNTQLNWVGTREREPGDTRAPLPGYRTVDFTLRTDLRSSPWELA
                     LSVRNVFNADVREPSPLTAPFVNLPFDIPLPGRSVFVQAVHRF"
     gene            complement(2597..3376)
                     /locus_tag="VM406_02355"
     CDS             complement(2597..3376)
                     /locus_tag="VM406_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74833.1"
                     /translation="MGILRSRMGAFFPGTHVIFRGHDLHTELKDMDWVELYVFGITGR
                     RFTSPQLRLLHAIWTTTSYPDGRIWNNRVAGLAGSVRSTGNLGMAAALAVSEASIYGR
                     GIDKRAIDFLIRTRQRVEAGERLEQCVREELDAHRSIAGYGRPVVNGDERNPHMLALC
                     RDLGLDQGAYLELALQIEHVLLEGRWRMKMNYGGLAAALCADLGLSPREYYLFMFPAF
                     LAGMPPCYIEASDREAGSLYPTPCDGVAYDGPARRQWHGGK"
     gene            complement(3415..4212)
                     /locus_tag="VM406_02360"
     CDS             complement(3415..4212)
                     /locus_tag="VM406_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HVL74834.1"
                     /translation="MSDNRTATSIRTKIWCEDHETDNRFAVRAAYCRGFDVYGDMLGR
                     ARWVDMLYLLFHAEPASVAQADLLEDLAFALANPGPRDASVHAAMCGGVGGSGTAAIL
                     MAALAVGAGQHGGGRDVYLAMQGWHACGADPDKWREWLAALPASPGSVWPDPSHAPGF
                     DSHAPRTSVPVRQLLSRLATHGCGWRLSFLAQSLARFEEMTCRGLSMAGVAAAAYSDL
                     GFSPEQGEALHLLLRLPGAAAHALEQRGLGYKTFPFGKMELQDAEGV"
     gene            complement(4233..>5829)
                     /locus_tag="VM406_02365"
     CDS             complement(4233..>5829)
                     /locus_tag="VM406_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HVL74835.1"
                     /translation="YTRESGRIGVCVATSGPGATNLITGVACAYDNGIPMLVITGQPA
                     LPLFGKRALQESACTGINTLEMFRHCTHYNSLVSHPDQLEAKLVAALMRATRTPRGPV
                     HLSIPLDILRSPTTLRAPSYDIGRLQQVPPLVSDDGVEHLGCHLIKARRIVILLGGGC
                     GAAAAGIVRLAQRLGAMVVTTPDGKGLIAPGHPLHAGVFGFAGHASAEAALRDPAVDL
                     ILAVGASMGEWTSAGWSEALLNGRLIHVDESDEHFSRSPMAKLHVQGHLPTLFERLTE
                     WLGPAGETQVSERLPQTDCPGDARARMGLREQAEELVHPHALMHVLGERLPPATRFLA
                     DAGNSMAWAIHYLKPRDWSARANESAAPDRDGDPAHIAGGWLRVTMDFAPMGWAIGGA
                     VGTALANPNVPVVCLTGDGSILMNGQEISTAVAEKLSVIFIVLNDAAFGMVKHGQRLS
                     GASRVAYELPRTDFAGLAAALGAHSCVVRSVDELATLDFPALCRRNGPTVVDVHIDGE
                     AIPPIGVRIQALRAAAVSSGTPD"
BASE COUNT         1085 a         1937 c         1841 g          966 t
ORIGIN      
        1 gggcgaacac ggcgcgcgca ccggctcgcc tgacgatcac tccgacgtca ttgggcgtca
       61 tgccagccgc gaccgcgata ctgcgaacct gaagccggga tgccgactcg atgccctcga
      121 tcatctggct gaacacgctg cgaaacggct cgccaagatc cgggtagagc acgacaagcg
      181 ttccggacgc ggcggcggct tccagcctgc tgtccgccag ctgcgcgagc aacggcgaag
      241 ccagcgtggg cgaatcgatt tcgggcaggc caggccagaa caacccgctt ctgcccatgc
      301 gcacacctgc acccgcgccc cactccccct tgccgtcggc ggcggctgga acgaaagcgg
      361 tgacaagccc gagcgcaagc ccaagacaaa cgcgcgcaag acgtggctgc ggagctggtc
      421 ttcggcggta tggagttgcc tgttccatca tcgatcaaaa gcgatgcacg gcctgcacga
      481 acacggagcg tcccggaagc ggaatatcga acggaagatt cacgaatggc gcggtcaggg
      541 ggctgggctc gcgcacgtcg gcattgaaga cattccggac ggacaaggcc aattcccagg
      601 ggctgctgcg caggtcggtg cgcagcgtga agtcgacggt gcggtagcct ggcagcggcg
      661 cacgcgtatc accaggctca cgttcgcgcg tgccgaccca gttcaactgg gtattgagca
      721 gccacccggg catgaagcgc cagtcggatc tcaggtagag cttggtgcgc ggcgcattcc
      781 ccgcattggc gccggtcatg ggatcgaacg cctcctggta ggccgcgttt ccggaaacac
      841 gaagggttcg cgcaaggtcc caggtcgcct cgatctcgag accgcgcccg gtttgcgagc
      901 cggcattcgc gaaatcgatc ccgaccaggc gcagcatgtc gctcatgcgg tactggtaaa
      961 cactggcccc cagctgaaga tcggggcgcg cctgccagtt caggaccgcc tcgatcgtgc
     1021 gcatgcgctc cggctgtacg ccgggattgc cgcgtccgac cgggttgttg atcgcataca
     1081 gctcggtaaa cgaaggcgcg cgaaacgccg agccgtacaa gagcttcgcc gtgaggttgt
     1141 atgccgcgtg ccacaccagc gcggcgcgcg gattggtagt gctgccgaag tccgaatagc
     1201 ggtcatgacg cacgccggca gtcagcgacc agtcggctgc gaaatcccat tcgtcctgca
     1261 ggtatacata tcggatattg cgactttgcg ggcgcaggaa tggcgccgac tcggtcacct
     1321 cgatgacatc cgcgcgcgag ccgctgccga tcggcatgaa gtcgggggtg tagtttctcc
     1381 actcgcgggt ccggtagatc gattcggcgg tataacctgc gcctacccgc acacgatgac
     1441 gttcgaaccc gttatagaac gccgaggccg acaatcgtcc ctggcgttcc cacttgtagg
     1501 gattcccgat gaaaccgtcg acaaacctgc cctcgaatgc gccggccgga taaaggacga
     1561 gatcggagaa ttcgcgatag cgcgtaaagc tcgcctgcac gtccaggtcc cagtcgggca
     1621 cgatgccgcg ctcgtggtat cccaggctgg tcgtcaggtt ctggctgtag ctgcgccccg
     1681 tcggatccag cgcctgagcc acgccaacgc cggaaccgac atcgcgccgt tccttgagtg
     1741 ccgtccacca gcgccaattg cgcagcctga gatcgaggtg ggcatcgatg gcgttgcgat
     1801 tgtcgttcat ccgacccggc gcgagcgagg catcggtatt gaacgccctg tcccaaccgg
     1861 tttgcgcgtc tgccgcgatc aggcgtcctg ctccgtccgt ggtgccgagt cgcaagtagc
     1921 cggcgatctc gaccggcccc gcctggccac cgtgaagaag ccaggcgtcg ccggtcttga
     1981 acgaccccgc ccgcaagccg gtctgcgtgc catcgatgtc gcgtgcattc ttggtgatga
     2041 tgttgatgac gcccgcgaac gcgtccgcgc catacagggc cgagcccggt ccgcgaatga
     2101 cttcgatgcg cgcaatgtgc tcgacaggaa agccgcccca gacgtcgccg cggtttccct
     2161 tgaacagacg gttcatcggg acgccattga gcaggatcag cacctgcgga ttgttctcat
     2221 ggtgcatgcc gcgaaacacg tagaccggcg cattcgtgat gacagccctt gagacgtgca
     2281 caccgggaac ggtctcaaga acctcgtcca gatccgtggc cccgatggcc ttgatgtcct
     2341 cggcggtgat gacggtggcg accgacggcg cccgcaccgt tggcatgcgg ccgccggttg
     2401 caatgctgac gtatgacttg tcgccgtaca tcaggagcag gtcgtcctcg tcggacattt
     2461 gcgctgctgc cagactgctc atgacaaggc ttatgacaag cacgagctgg cgcggatgct
     2521 tcgcatccat acactcccct tcaacgatcc ctatacaact tgtccgactg cctgggcggg
     2581 cggatgccga ccctcactat ttaccgccat gccattgccg ccgtgccggg ccgtcgtaag
     2641 cgacgccgtc gcatggggtc ggatacaggc tccccgcttc ccggtcggag gcctcgatat
     2701 agcaaggcgg catgcccgcc agaaaagccg gaaacatgaa caggtaatat tcgcggggcg
     2761 acaggccaag gtccgcgcac agcgccgcgg cgaggccgcc gtagttcatc ttcatgcgcc
     2821 aacgaccttc gagcagcaca tgctcgatct gcaacgccag ctcgaggtac gccccctggt
     2881 caagccccag gtcgcgacac agggcaagca tgtggggatt gcgctcatcc ccgttcacca
     2941 caggccggcc gtaaccggca atgctgcggt gcgcgtccag ttcctcgcgg acgcactgct
     3001 caaggcgctc tccggcctcg acgcgctgcc gtgtcctgat caggaaatcg atggcgcgct
     3061 tgtcgatccc gcgaccatag atcgacgctt ccgataccgc cagagccgct gccatgccga
     3121 ggttgcccgt gctgcgcaca cttcccgcca ggcccgcgac gcggttgttc cagattcgcc
     3181 catccggata actggtggtt gtccagatgg catggagcag gcgcagctgc ggtgaagtga
     3241 agcgacgccc ggtgatgccg aagacgtaga gctccaccca gtccatgtcc ttcagttccg
     3301 tatgcaggtc gtgcccgcgg aagatgacat gcgttcccgg aaagaatgcg cccatgcgcg
     3361 aacgcaggat gcccacatgc tcgtcaagcg cgtgtggcgc cgctcccgat acgttcatac
     3421 gccctccgca tcttgcagtt ccatcttccc gaatgggaag gtcttgtaac ccagcccgcg
     3481 ctgctccagc gcatgcgccg cagcccccgg caggcgcagc agaagatgga gcgcctcgcc
     3541 ctgctcggga ctgaaaccaa gatccgagta tgccgctgcg gcgacaccgg ccatcgacag
     3601 gccgcggcag gtcatctcct cgaaacgggc aagcgactgg gccagaaaag aaagtcgcca
     3661 cccgcaaccg tgcgtggcca ggcggctcag caactggcga acgggcacgc ttgtgcgagg
     3721 agcatgcgag tcgaatcccg gggcatgcga aggatccggc cacacgctgc ccggcgatgc
     3781 cggcaaggcg gcgagccact cgcgccactt gtccggatcg gctccgcacg cgtgccatcc
     3841 ctgcatggcg agatagacgt cgcgcccgcc gccatgctgc ccggcgccca cggcaagggc
     3901 cgccatcagg atggctgcgg tgccggaccc gccgacgcct ccgcacatgg cggcgtgcac
     3961 cgaggcgtcg cgaggccccg gattggcgag cgcgaaggcc aggtcttcca gcaggtcggc
     4021 ctgcgccacg ctggccggct cggcatggaa cagcaggtag agcatgtcca cccatcgcgc
     4081 ccgcccgagc atgtctccat acacgtcgaa gccgcggcag taagccgccc gaacagcaaa
     4141 acggttgtcg gtctcgtgat cctcgcacca tatcttggtg cggatgcttg tcgcagtcct
     4201 gttatcgctc atggcgtcgt tgtccgaagg tttcaatcgg gagtgccgga ggataccgcc
     4261 gccgcacgca acgcctggat gcgcacacct atcggcggga ttgcctcgcc gtcgatatgc
     4321 acgtccacca cggtgggccc gttgcggcga caaagcgccg ggaaatccag ggtggcgagt
     4381 tcgtcgacgc tgcgaaccac acacgagtgg gcaccgagtg cggctgccaa gccagcgaaa
     4441 tcggtccggg gcagctcata cgccacgcgg ctcgcaccgg aaagccgttg cccgtgcttg
     4501 accatgccga acgccgcgtc gttcagcacg atgaatatca ccgagagctt ctctgcgacg
     4561 gcggtggaaa tttcctgccc attcatcagg atgctgccgt cacccgtcag gcagacgact
     4621 ggcacgttcg ggttcgccag cgcagttccg accgcaccgc cgattgccca gcccatcggg
     4681 gcaaaatcca tggtgacgcg caaccagcct ccagcgatat gcgccggatc gccgtcgcga
     4741 tcgggcgctg cgctctcgtt tgcccgtgca gaccaatccc gcggcttaag atagtgaatg
     4801 gcccatgcca tgctgtttcc cgcatcggcg agaaaacggg tcgcgggggg cagacgctcg
     4861 ccaaggacat gcatcagcgc gtgcgggtgt accaactctt ctgcttgttc acgtagaccc
     4921 atgcgagcac gcgcatcgcc cggacaatca gtctgcggca agcgctcgct gacttgcgtc
     4981 tccccggccg ggccgagcca ctccgtcagg cgttcgaaca gggttggcaa atgaccctgc
     5041 acatgcagct tcgccatcgg actgcgcgaa aaatgctcgt ccgattcgtc gacgtgaatg
     5101 agcctgccgt taagcagtgc ttcactccat cctgcgctcg tccattcccc catgctggcg
     5161 ccgaccgcaa gaatgagatc gacggccgga tcacgcaggg ccgcctcggc gctcgcatgg
     5221 ccggcaaagc cgaacacgcc cgcatgcaat ggatgacccg gagcgatcaa gcccttgccg
     5281 tccggagtgg tgacgaccat tgcccccaag cgctgcgcaa gccggacgat gcccgcggct
     5341 gcggcgccac agccaccccc gagaagaatc acgatcctgc gcgccttgat gaggtgacag
     5401 ccgagatgct cgacgccatc gtcactcaca agcggcggca cctgctgcaa gcggccgatg
     5461 tcgtaggacg gcgcgcgcaa cgtcgtgggg ctgcgcagga tgtccagcgg aatggaaaga
     5521 tgcaccggcc cgcgcggtgt gcgcgtcgca cgcatcagtg cggcgacgag cttggcctca
     5581 agctggtccg gatgcgaaac gagtgagttg tagtgcgtgc agtggcggaa catttccagc
     5641 gtgttgatgc cggtgcaagc cgactcctgg agcgcacgct tgccgaacaa cggcagcgcc
     5701 ggctggccgg tgatgacgag catcgggatg ccgttgtcat aggcgcaggc cacgccggtg
     5761 atcagattgg tcgcgcccgg accggaagtg gcgacgcata ccccgatccg gccgctctcg
     5821 cgggtatag
//
LOCUS       DATLGF010000082         6672 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_130643, whole genome shotgun sequence.
ACCESSION   DATLGF010000082 DATLGF010000000
VERSION     DATLGF010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6672
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130643"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..669
                     /locus_tag="VM406_02370"
     CDS             46..669
                     /locus_tag="VM406_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74836.1"
                     /translation="MRRLWAVGQDPLEREASRALLARAAARFTPDAELEADAGNLIAS
                     MLAAGLDREAAAWVPVVNRIDNHNGDSAWAMLALAAPDTNGLDVSAGRVNAFITRDTS
                     PGRKRSALLVAGLAGLGRLDLDAADRLNSRHNLGLDRRSRWTRVIDSSAALGQPATVL
                     LLTGTGFQTSLFEQLPSAHLYHSVAALRRTGQDFTARMIAAEALFRT"
     gene            666..1553
                     /locus_tag="VM406_02375"
     CDS             666..1553
                     /locus_tag="VM406_02375"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HVL74837.1"
                     /translation="MTDEDHALVDRFLDMLAAEAGASKHTLAAYRSDLAQASADLGEP
                     MGQATSAAISGLGESWSHLAPSTVARRAAALRRFFAFLVDEGCRVDDPSDALPKPALQ
                     RPLPRVLDEQEVTRIFETASALAASGRPTAVRNLALLELLYGSGLRASELVSLPRGAL
                     RKGQPFLILRGKGSKERLVPISSRAEQAVEEWRAHVPLASPWLFPGGKAHISRVRLFQ
                     IVREIAAQVGISPQRVSPHVLRHAFATHLLSGGADLRVLQSLLGHADIGTTQIYTHVD
                     SGRLVELVNARHPLATRGR"
     gene            1588..2529
                     /locus_tag="VM406_02380"
     CDS             1588..2529
                     /locus_tag="VM406_02380"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HVL74838.1"
                     /translation="MLTFLDFEKPIAELEARVSELRETANAGELDLDQEIARLEAKSA
                     KLLRETYAKLTPWQKAQVARHPDRPHFKDFVAGLTDDFMPLAGDRSFADDPAIVGGLA
                     RVDGRRVMLIGHEKGDDTASRLKHNFGMAKPEGYRKAIRLMELADRFGVPVVTLVDTP
                     GAFPGVQAEERGQAEAIARSTEQCLALGVPMIAAIVGEGGSGGAVAISAANRVLMFEH
                     AVYSVISPEGCASILWRTAEKAAEAAEAMRITANDLQSLGVIDRIIQEPLGGAHRDPT
                     AAIASLKAAIVEELNGCSKLGRAELAAQRRAKFLNMG"
     gene            2609..4078
                     /locus_tag="VM406_02385"
     CDS             2609..4078
                     /locus_tag="VM406_02385"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HVL74839.1"
                     /translation="MMRTPLSLLAATAVVLAPAAAAQTGRALNPRDVATAQRQHAELV
                     AEFGGAETGARGAYVESVGRRVAAFSGITNPAGVFRFTALNSAVENAFADPGGYVYIT
                     RQLMTVMNDEAELAFVLGHETAHIAAMHGRARQSAATRNTIGGILGAIVGSVLGGGFG
                     SAIAQLSQQAAQLYTLSFSREQEYQADVLGIRYMTSAGYDPGGAADMLAALTRSSAIQ
                     ARVQGRTNRQVPEWASTHPLSENRTQQARAEAQRTGRLGTGVRNRDQFLAQLNGVYVD
                     DDPAQGIIEGRTFTHPDLMMQFAVPVGYLMQNSTRAVGIRGSAGQAQFSGGRFGGSIE
                     QYIHQVLQQLTGGRVQMAVPPPRRTVINGMPAAFTTARANTSSGAVDVSVVAYEWDRD
                     TKYHFVMLTQAGTGVGPFTPMVNSLRRITPAESAAIRPRVIQIVSVGPNDTVHSLAGR
                     MAYRDFQLDRFLALNNLPPNARLVPGQRVKLVVFGHRRA"
     gene            complement(4101..4280)
                     /locus_tag="VM406_02390"
     CDS             complement(4101..4280)
                     /locus_tag="VM406_02390"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HVL74840.1"
                     /translation="MGAIRRILRRLLADDSGATAIEYGLIASLIIIAMMGGLATLGGG
                     AGGMWTKIQEAVVSS"
     gene            complement(4385..5164)
                     /locus_tag="VM406_02395"
     CDS             complement(4385..5164)
                     /locus_tag="VM406_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVL74841.1"
                     /translation="MVRQLFDMRLRATRRDRAQRIGTALFLHERAFEDCLERLQGIRR
                     RFQSALLIGCPDPNWPRRLGVLTGHVDVADPGPMFAAAADGELVIEDEADFPSGAYDL
                     VVAIGTLDSVNDLPAALQLIKDALRPDSLLIGAIVGGDSLPHLRAAMREADIAMGVAS
                     PHVHPRIEASALAALLSGAGFVMPVVDVDRVDVSYASLGRLVADLRAMAGTNLLSERD
                     RQPLTRRAYAAACTAFAEAGDGNRTLERFEILHFACWTASR"
     gene            5142..5945
                     /locus_tag="VM406_02400"
     CDS             5142..5945
                     /locus_tag="VM406_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HVL74842.1"
                     /translation="MSNSWRTIRPLVRRASLGDKSLMIAHAAIGSGFRHLLDFALPAR
                     CAGCGIIVDEIHSFCGDCWKQIEFLAGGCTCCGLPLKGTDIETCVACLAKPPRIERTR
                     AAVAYDDKSRSIALRLKYSRRVALARTMARYMAPLVDRDAAKPVLVPVPLHRLRLWNR
                     GFNQSALVAQELSRRIGFPHDPFLLRRVKRTPPLKGLSQLQRRKTIAGAFAVHKGACL
                     NGATVILVDDVLTTGSTANACARVLQRAGADRVHLVTWARVVRPNPFTR"
     gene            5972..6229
                     /gene="grxC"
                     /locus_tag="VM406_02405"
     CDS             5972..6229
                     /gene="grxC"
                     /locus_tag="VM406_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018574392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HVL74843.1"
                     /translation="MVKVEIYTKAYCPYCYRAKALLDSKKVEYQEHAVDGGGPTRQEM
                     IQRAGGRTTVPQVFIDGRHIGGCDDLYDLERGNMLDKILSA"
     gene            6233..>6672
                     /locus_tag="VM406_02410"
     CDS             6233..>6672
                     /locus_tag="VM406_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HVL74844.1"
                     /translation="MTRIALFQSCTGIAPMANADALVAAVGQAAAGGAEMLFTPEMSG
                     LLDRDASRAASSLREEKDDPVLAACREAASRHGLWLHLGSLAVKSGEGKLANRAFVIN
                     PQGEVLARYDKIHLFDVDLPTGESWRESAIYRAGQDAVLVQETP"
BASE COUNT         1197 a         2110 c         2165 g         1200 t
ORIGIN      
        1 cggctggcct ttgccggaag gacggaacag gctcggctcg aggccatgcg gcgcctgtgg
       61 gcggtgggcc aagacccgct ggagcgcgaa gcatcgcgcg ccttgcttgc gcgagcggcc
      121 gcaaggttca cgcctgacgc agaactggaa gcggatgccg gtaatctcat cgcatcgatg
      181 ctggcagcgg gcctcgaccg ggaagccgcg gcttgggtgc cggtcgtcaa tcgcatcgac
      241 aatcacaacg gtgatagtgc ttgggcaatg ctggccctcg cggcgccgga cacgaacgga
      301 ctcgacgtga gcgccggccg cgtgaatgcg ttcatcactc gcgacaccag cccgggccgc
      361 aagcgcagcg cactcctcgt tgcgggtttg gcaggcctag ggcgccttga cctcgacgca
      421 gccgaccggc tcaacagccg ccacaacctt ggactcgacc gcaggagccg ctggacccgg
      481 gtcatcgaca gttccgccgc cctcggccag cctgcgaccg tgctcttgct aaccggcacc
      541 ggctttcaga cctcgctttt tgagcaactg ccatcagcgc atctctatca ttcggttgcg
      601 gcgctgcggc gcacgggaca ggatttcacc gcgcgcatga tcgcggcaga ggcgctcttc
      661 cgtacgtgac cgacgaggac cacgcgctgg tcgaccggtt cctcgacatg ctggccgccg
      721 aggcaggggc ctcaaagcat acgctggcgg catatcgcag cgaccttgcg caggcttccg
      781 ccgatctagg tgaaccgatg ggccaggcga ccagcgcagc catttccgga ctgggagaaa
      841 gttggtccca ccttgcccct tcgactgtgg cccgtcgagc cgcggccctg cggcgcttct
      901 tcgcctttct cgtcgacgag ggatgccggg tggatgatcc ctctgatgct ttgccgaagc
      961 ccgcgctgca gcgacccctg ccgcgggtgc ttgatgagca ggaagtaacg cgcattttcg
     1021 agacggcgag tgcccttgcg gcgagtggtc gccccaccgc tgttcgaaat ctcgccttgc
     1081 tcgagctgct ttatgggtct gggctgcgcg ccagcgaact cgtcagcttg ccgcgcggcg
     1141 cgcttcgcaa gggccaacca tttctcattc tccgcggcaa gggcagcaag gagcgcctgg
     1201 tgccaatttc ctcgcgagcc gagcaagcgg tggaagagtg gcgcgcgcat gtcccgctgg
     1261 cgagcccatg gctgtttccc ggcgggaagg cgcacatcag ccgagtccgg cttttccaga
     1321 tcgttcgaga gatcgccgcg caggtgggga tttccccgca gcgcgtcagt ccgcatgtct
     1381 tgcgccacgc ttttgccacg cacctgctat caggcggtgc agacctccgc gttctccagt
     1441 cgctattggg tcatgcggac attggcacga cgcagatcta cactcatgtc gatagcgggc
     1501 ggctcgtgga gctggtcaac gccaggcacc ccctggctac ccgcggtcgt tgacggcgcg
     1561 gcagccccgt cctaggcgcc cgcggcgatg ctcaccttcc tcgactttga gaagccgatt
     1621 gccgagctcg aagctcgggt ctccgaactt cgggagacgg cgaatgcggg ggaactcgac
     1681 ctcgaccagg agattgcccg gcttgaagcg aagtcagcaa agcttctccg tgaaacctat
     1741 gcgaagctga cgccttggca aaaggcgcag gtcgcccgcc atccggaccg gcctcacttc
     1801 aaggatttcg tcgccgggct gaccgacgat ttcatgccgc tggcagggga caggtcgttt
     1861 gcggatgacc cggcgatcgt cggcggtctc gcgcgggtcg atggtcggcg ggtgatgctg
     1921 atagggcatg agaagggcga cgacaccgcc tcgcggctca agcacaattt cgggatggca
     1981 aagcccgagg gctaccgcaa agccatccga ttgatggaac ttgcggaccg ttttggagtg
     2041 ccggtcgtga ctttggtcga cacgccgggc gcctttcccg gtgtccaggc tgaggagagg
     2101 gggcaggccg aggcgatcgc ccggtcaacc gagcaatgcc ttgcgctggg agtgccgatg
     2161 atcgcggcaa tcgttggtga aggcggttcc gggggcgccg tggccatctc tgcggcgaat
     2221 cgcgtgctga tgttcgaaca tgccgtctat tcggtcattt ctcccgaggg atgtgcatcg
     2281 atcctttggc ggaccgctga gaaggcagct gaagcggccg aggcaatgcg gatcaccgcc
     2341 aacgatctgc agtcgctcgg tgtgatcgat cggatcatcc aggagcccct cgggggcgcg
     2401 cacagggacc ccaccgccgc catcgcctcg cttaaagcag cgattgtcga ggaactgaac
     2461 ggctgctcga agttgggccg ggcggagctt gcagcccaaa gaagggccaa gttcctgaac
     2521 atgggttagc ccggcaagga ggaaccagcc gccgtcatct cccgttcaaa ccgcaaggct
     2581 tacgagcctc cagcgaactg gagagagaat gatgcgcacc cccttgtccc tgctggcggc
     2641 gacggccgtc gtgcttgcgc ccgccgccgc ggcgcagacc ggccgagcgc ttaatccacg
     2701 cgacgtcgcc actgcgcagc gccagcacgc cgagctggtc gctgaattcg gcggtgcgga
     2761 aaccggcgcc cgcggcgctt atgtcgaatc cgtcgggcgc agggtcgcgg ccttttcggg
     2821 cattaccaac ccggccggag tcttccggtt cacagcgctc aattcggcgg ttgagaatgc
     2881 tttcgccgac ccgggcggat acgtctacat cacgcgccag ttgatgaccg tcatgaacga
     2941 tgaggcggag ctcgccttcg tcctcgggca tgagacggcc cacatcgcag cgatgcacgg
     3001 cagggctcgc cagtcggccg cgacgcgaaa caccatcggt ggcatccttg gcgccatcgt
     3061 tggaagtgtc ctcggaggcg gcttcggcag cgccatcgcc cagctcagcc agcaggcagc
     3121 gcagctctac actttgagct tttcgcgcga gcaggaatat caggcggacg tcctcggaat
     3181 ccgctacatg acctcggccg gctacgatcc aggcggggcg gctgacatgc tcgcggcgct
     3241 cacccgttcg agcgcgattc aagcccgggt tcaaggccgc accaaccgcc aggtcccgga
     3301 gtgggcgagc acccacccct tgagtgaaaa tcgcactcag caggcgcgcg cggaagcgca
     3361 gcggactggc cggcttggca cgggtgtgcg caatcgcgac cagttcctcg cccagctcaa
     3421 cggcgtgtat gtcgatgacg atcctgccca gggcattatc gagggacgca cattcaccca
     3481 tccggacctg atgatgcagt tcgctgtgcc ggtgggttat ttgatgcaga acagcacccg
     3541 tgcggtcggc attcgcggct cggcggggca ggcgcagttc agcggcggcc gatttggcgg
     3601 gtcgatcgag caatatatcc atcaggtgct tcaacagctt accggaggca gggtccagat
     3661 ggccgtgccg ccgccgcggc gcaccgtgat caatggcatg cccgcagcct tcacaacggc
     3721 acgcgcgaat acgtcctcgg gcgctgtgga tgtcagcgtc gttgcctacg aatgggatcg
     3781 cgacacgaaa tatcacttcg tcatgctgac gcaggcaggg accggcgtcg ggccgttcac
     3841 gccaatggtc aactcgctgc ggcgcataac gcctgcggaa tccgcagcga taaggccgcg
     3901 ggtgatccaa atcgtgtcgg ttgggccgaa cgatacggtg cattcgctgg ccggccgaat
     3961 ggcctaccgc gatttccaac tcgaccgttt tctcgcgctg aacaaccttc cacccaatgc
     4021 tcgcctcgtg ccgggtcaac gggtgaagct ggtcgtcttc gggcaccggc gggcgtaggt
     4081 ggcgttctgc aggccgaagt ctagctgctt acgacggctt cctggatctt cgtccacata
     4141 cctcccgcgc ctccgccgag agtcgccaat ccgcccatca tcgcaatgat gatcagggag
     4201 gcgatgagcc catattcgat cgcggtcgcg ccgctgtcgt cagcgagcaa cctgcgcaaa
     4261 atccttcgga tagcgcccac gccaccctcc tctggtccac gtccacgacg caaacttacc
     4321 ggcagctggg ttaacaactc ctcccataga agcgttaacg gcggcaaatg tgtgccgaaa
     4381 cgtcttagcg cgaggcagtc cagcaggcga aatgcaggat ctcgaatctt tccagcgttc
     4441 ggttgccgtc tccggcctct gcgaacgcgg tgcaagccgc tgcatatgct ctgcgggtga
     4501 gtggctggcg gtcccgttcc gaaagtaaat tggtcccagc catggcccga aggtcggcta
     4561 caaggcggcc gagcgatgca tagctgacgt ccacccgatc gacgtcgacc accggcatga
     4621 cgaaccctgc gccggacagc aaagccgcca gcgcagaagc ttcaatgcgc gggtgtacgt
     4681 gcggggacgc aacgcccatg gcgatgtcgg cttctcgcat cgcggcgcgg aggtgaggaa
     4741 ggctgtcgcc accgacgatc gcgccgatca gcagggaatc cggacgcagg gcatccttga
     4801 tcagttggag cgcggccggc agatcattga cgctatcgag cgtgccgatg gctacgacca
     4861 ggtcatacgc gccggatggg aagtctgctt cgtcttcgat gacgagctcg ccgtcagccg
     4921 ctgcggcgaa cataggcccg gggtcggcta cgtcgacatg gcccgtcagg accccgagac
     4981 gccggggcca gttcggatcc gggcagccaa taagcagggc ggattggaat cgacggcgga
     5041 ttccttggag ccgttccagg cagtcttcga aagcccgctc gtgcaggaac agcgcagtcc
     5101 cgatccgttg ggcacgatcc cgtctcgttg cgcgcaatcg catgtcgaac agctggcgta
     5161 ccattcgacc gcttgtgcga cgcgcgtcgc tgggcgacaa gtcgctgatg atcgcccatg
     5221 cggcaattgg ttcaggcttc cggcacctgc tcgacttcgc cctgccggca cggtgcgcag
     5281 gctgcgggat catcgtcgac gagattcaca gcttttgcgg cgattgctgg aagcaaatcg
     5341 aatttcttgc gggcggatgc acctgttgcg gcctaccgtt gaaaggcacc gatatcgaga
     5401 cctgcgtcgc gtgcctcgcg aagccaccgc gtatagaacg gacgcgggcc gcagtcgcgt
     5461 atgacgacaa gtcccgaagc atcgcgctgc gcctgaaata tagccgaagg gtcgcgctgg
     5521 cgaggaccat ggcgcgttac atggcgccgc tggtggatcg cgacgctgca aagcccgtgc
     5581 tggtgccggt tccgcttcac cggttgcggc tgtggaatcg tggattcaac cagtccgcgc
     5641 tcgtcgccca ggaactttcg cggcgcattg ggtttccgca cgacccattt ttgctccggc
     5701 gcgtgaagcg cacgccgcca ctcaagggct tgtcgcaatt gcaacgacgc aagacgattg
     5761 ccggagcctt tgccgtgcac aagggcgcct gtctgaacgg cgccaccgtg attctcgtcg
     5821 atgacgtgtt gaccaccgga agcactgcga atgcctgcgc gagggtgctt cagcgagcgg
     5881 gggcggaccg cgttcatctg gtaacgtggg cccgggtggt gcgaccgaac ccgttcacgc
     5941 gttaggtctg ccccaataaa ggtgagcatc tgtggtcaaa gtggaaatat ataccaaggc
     6001 ttactgcccc tattgttacc gcgccaaggc cctgctggat tcaaagaagg ttgaatatca
     6061 ggagcatgcc gtcgacggcg gcggaccaac caggcaggaa atgattcagc gcgctggcgg
     6121 acgcacgacg gtgccgcagg tcttcatcga tggcaggcat atcggcggct gcgacgatct
     6181 gtatgatctc gaacgtggaa acatgctgga caagattctc tcagcctgat cgatgacccg
     6241 aattgcactc tttcaatcct gcaccggcat cgccccgatg gcgaatgccg acgccttggt
     6301 cgctgccgtg ggacaggccg cggccggcgg cgccgagatg cttttcacgc cggaaatgtc
     6361 gggcttgctc gaccgcgatg cttcacgcgc tgcttcaagt cttcgcgagg agaaagacga
     6421 tccggtactg gcggcgtgcc gggaagcggc atcgaggcat ggattgtggc tgcacctcgg
     6481 ctcgctggcc gtcaaatcgg gtgaggggaa gctggcgaac cgagcgttcg tcatcaatcc
     6541 gcagggtgaa gtgctggcgc gctacgacaa gatccatctg tttgacgtgg atcttccaac
     6601 gggcgagagc tggcgcgagt ccgccattta ccgcgccggc caggacgcgg tgcttgtcca
     6661 ggagacgccg at
//
LOCUS       DATLGF010000083         6741 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_97735,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000083 DATLGF010000000
VERSION     DATLGF010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6741
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97735"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="VM406_02415"
     CDS             <1..995
                     /locus_tag="VM406_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVL74845.1"
                     /translation="TQVIAAAGLSVVIVIALVQAANNETSVGGFVSFITAMLMLLTPL
                     KRLAELNGPLQRGLAAADSVFELIDASSERTSGRTLDGRAAGRIDFVDVGFAYPGRPE
                     EALSGINLAIQPGETVAFVGMSGGGKSTLVNLVPAFYEPTRGEVRLDGEPIADLALTS
                     LRAQIAMVSQNVVLFDDTVAANIAYGDANPDMARVQAAARAAHLDGVIAALPDGFDTM
                     IGDNGNKLSGGQRQRLAIARAIYKDAPILILDEATSALDTESERAVQAALDELMRGRT
                     TLVIAHRLSTIERADRIVVLVGGRIVETGTHVELLEKNGAYANLYHLQFEKETS"
     gene            995..1585
                     /locus_tag="VM406_02420"
     CDS             995..1585
                     /locus_tag="VM406_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HVL74846.1"
                     /translation="MSNTPAISRFPVPDVNELPDDIKEMILAVQEKAGFVPNVFLTLA
                     HRPAEFRAFFAYHDALMLKETGSLSKADREMIVTATSAKNSCLYCVVAHGALLRIYAK
                     QPLVADQVAVNHAKADITPRQKAMLDFAIKVCLRSHEVGDEDYALLRSHGFDDEDIWD
                     IAAITAFFGLSNRMANVISMRPNDEFYLMGRVPRSK"
     gene            complement(1794..3017)
                     /gene="hutI"
                     /locus_tag="VM406_02425"
     CDS             complement(1794..3017)
                     /gene="hutI"
                     /locus_tag="VM406_02425"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HVL74847.1"
                     /translation="MNETWDALWTNVHLATMEGAGYGEVQDGAIAVRDGRIAWLGRRA
                     DLPDGQRARKHHDGQGRWLTPGLIDCHTHIVHAGNRSMEFEARLNGASYEEIARAGGG
                     IMATVGATRAASEEALLTASMRRARQLIAEGVTTIEIKSGYGLDLENERKMLRVARRL
                     GEHLPVRVVTTFLGAHALPPEYSGRPDDYIDAVCDQLAVLADEGLVDAVDAFCERIGF
                     SREQTARVFDAARRHGLPVKLHAEQLSDQDGAALVARYRGLSADHLEYVSQTGIAAMA
                     RAGTVAVLLPGAFYFLRETRLPPVDALRVAGVPIAVATDCNPGTSPMTSLLLAMNMAC
                     TLFRLTPQEALAGTTIHAARALGLQDELGSMRVGKRADLALWDVERPAELAYAIGGNP
                     CVGVVRGGDALTRVE"
     gene            complement(3010..3996)
                     /gene="hutG"
                     /locus_tag="VM406_02430"
     CDS             complement(3010..3996)
                     /gene="hutG"
                     /locus_tag="VM406_02430"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050415 - formimidoylglutamase activity
                     [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HVL74848.1"
                     /translation="MIRQPDMRAWQGRVDAEEGPLGQRWHQIVRPFEAADAAGSVALI
                     GFACDAGVARNHGRTGARGGPAALRATLSNLPARDCNALRDAGDVICTPDDGGDGLEA
                     AQAELALRIAGLLDAGALPLGMGGGHEIAWASFSGLATHLAATEATPRIGILNLDAHF
                     DLRMASAPSSGTPFRQIAEDCAARGWPFHYCCLGVSDYANTQALFARARQLGVTWLRD
                     EDMDITRRDQVLRTLDTFLVGVDHLYLTVCLDVLPAAVAPGVSAPAARGVPLEVIEPV
                     IDRAVASGKLRVADIAELNPDRDIDQRTARVAARLIARIARAHAADKEGRHE"
     gene            complement(4005..5540)
                     /gene="hutH"
                     /locus_tag="VM406_02435"
     CDS             complement(4005..5540)
                     /gene="hutH"
                     /locus_tag="VM406_02435"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HVL74849.1"
                     /translation="METKLIPGQLTLDMLRRIWRGPVTLALEPSAYEAIHASAATIRR
                     ILERGAPAYGINTGFGKLAKTHIPDAQLEALQRNLILSHSVGTGALLDDRVVRLILAT
                     KIGSLARGYSGIRASVIDTMIAMFNAGILPCIPSKGSVGASGDLAPLAHMTLAMMGEG
                     QVRVGGNVVPASAALAQANIEAVRLAPKEGLALINGTQVSTALALDGLFMAERMLEAG
                     TVAGALSVDAAKGSDAPFDPRIHAVRGQPGQIALARVYRELLAGSEIRRSHLDNDERV
                     QDPYSLRCQPQVMGACLDVINHAARTLLTEANAVTDNPLVYADSGDVISGGNFHAEPV
                     AFAADTLALAIAETGALAERRIALLIDSTLSGLPPFLVASSGLNSGFMIAHVTAAALA
                     SENKSLAHPASVDSLPTSANQEDHVSMATFAARRLHEMAENTATILGIELLAAAQGID
                     FHRPLATSPRLAAVHAHLREHVPFYDKDRLFAPDIEHARGIVLDGALSRDCGDLFNGL
                     YIG"
     gene            complement(5577..>6741)
                     /gene="hutU"
                     /locus_tag="VM406_02440"
     CDS             complement(5577..>6741)
                     /gene="hutU"
                     /locus_tag="VM406_02440"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HVL74850.1"
                     /translation="GGMGGAQPLAATLAGAVSLNIECQQSRIDFRLRTRYLDKQARDL
                     DHALELVREHCARKEAVSIGLLGNAAEIFPELVKRAQAGGMRPDLVTDQTSAHDLING
                     YLPRGWSVEQWKAAQNDPQQHARLGAEAAASCAVHVRAMLDFHAMGIPTVDYGNNIRQ
                     VALDQGVEHAFDFPGFVPAYIRPMFCEGKGPFRWVALSGDPEDIYRTDAKIKELFPEN
                     RHVHRWLDMARERIAFQGLPARICWLGLGERHRAGLAFNEMVKNGELKAPIVIGRDHL
                     DTGSVASPNRETEGMRDGSDAVSDWPLLNALINTAGGATWVSLHHGGGVGMGYSQHAG
                     MVIVADGSEAAARRLGRVLVNDSGSGVMRHADAGYEAAVACAQRNGLKLPMVK"
BASE COUNT         1087 a         2257 c         2280 g         1117 t
ORIGIN      
        1 tgacccaggt gatcgcggcg gcgggcctgt cggtcgtgat cgtgatcgcg ctggtccagg
       61 cggcgaacaa cgagacgtcg gtcggcggct tcgtctcgtt catcaccgcg atgctgatgc
      121 tgcttacgcc gttgaagcgc ctggcggaac tgaacggccc gttgcagcgc ggcctcgccg
      181 cggccgactc ggtgttcgag ctgatcgacg cgtcgagcga acgcacgtcc gggaggacgc
      241 tggatggccg cgccgcaggg cgcatcgatt tcgtcgacgt gggcttcgcc tatccgggcc
      301 ggccggaaga ggcgctctcc ggcatcaacc tcgccatcca gccgggagag acggtggcct
      361 tcgtcggcat gtccggcggc ggcaagtcga cgctggtgaa cctggtgccg gccttctacg
      421 aaccgacgcg aggggaagtg cggctagacg gcgagccgat cgccgatctt gcgctgacca
      481 gcctgcgcgc gcagatcgcg atggtgagcc agaacgtggt gctgttcgac gacacggtcg
      541 cggcgaacat tgcctatggc gatgcgaatc ccgacatggc gcgcgtgcag gccgccgcgc
      601 gggcggcgca tctcgatggc gtgatcgccg cactgcccga tggcttcgac accatgatcg
      661 gcgacaatgg caacaaactg tccggcggcc agcgccagcg cctggcgatc gcccgcgcga
      721 tctacaagga tgcgcctatc ctgatccttg acgaggcgac atcggcgctg gataccgaat
      781 ccgagcgcgc ggtacaggct gcgctggatg agttgatgcg cggacgcacg acgctggtga
      841 tcgcgcaccg gctgtcgacg atcgagcggg ccgacaggat cgtcgtgctg gtcggcggcc
      901 gtatcgtcga gaccggcacg catgtggaat tgctggagaa gaatggcgcc tacgccaacc
      961 tgtatcactt gcagttcgaa aaggagacct cgtgatgtcg aacacccctg cgatttcccg
     1021 ttttcccgtt ccggatgtga atgaattgcc ggacgatatc aaggagatga ttctcgcggt
     1081 gcaggagaag gccggcttcg tgccgaatgt cttcctgacg ctggcgcatc gcccggccga
     1141 gttccgcgcg ttcttcgcgt atcacgatgc gttgatgctg aaggaaaccg gcagcctgag
     1201 caaggccgac cgcgagatga tcgtcaccgc caccagcgcg aagaacagct gcctgtactg
     1261 cgtggtcgcg cacggcgcct tgttgcgcat ctatgcaaag cagccgttgg tcgcggacca
     1321 ggtcgcggtc aaccatgcga aggccgacat cacgccacgc cagaaggcga tgctcgattt
     1381 cgcaatcaag gtctgcctgc gctcgcacga ggttggcgat gaagactatg cgcttctgcg
     1441 cagccatggc ttcgacgacg aggacatctg ggatatcgcg gcgatcaccg cattcttcgg
     1501 tctctcgaac cggatggcga acgtgatctc gatgcggccg aatgatgagt tctacctgat
     1561 ggggcgggtg ccgcgcagca agtgagcggg cgggttttcc gcgcttggtc agacgtgaac
     1621 cggttcgaac gtctgtagta ctgcatgcga ctaagccggg ggcgtccggc taaacactgc
     1681 catcccgaac gcagtgaggg atctcatccg ttcagtaagc aaggcgcgca gcacgtggaa
     1741 gaagctcctg tctcgctcca ctgctcagcg accccgtctt acggttaccg tcatcactcc
     1801 acccgcgtca acgcgtcccc acccctgacc accccgacac agggattccc tccaatcgca
     1861 taggccagtt ccgccggccg ctccacgtcc cacaacgcaa gatccgcgcg cttgccgacg
     1921 cgcatgctgc ccagctcgtc ctgcaggccg agcgcgcgtg cggcgtggat cgtggtgccg
     1981 gccagcgctt cctgcggtgt gagacggaac agcgtgcagg ccatgttcat cgccagcagc
     2041 agcgaagtca tcggcgaagt gccggggttg caatcggtcg caactgcgat cggcacgccg
     2101 gccacgcgca gcgcatccac cggcgggagc cgggtctcgc gcaggaagta gaacgcgccg
     2161 ggaagcaaca ccgcgacggt gccggcgcgc gccatcgcgg cgatcccggt ctgcgacaca
     2221 tattcgaggt gatcggccga cagcccgcgg taacgcgcga ccagcgccgc gccgtcctgg
     2281 tccgacaact gctccgcatg cagcttgacc ggcagaccat ggcggcgtgc cgcgtcgaag
     2341 acgcgcgcgg tctgctcgcg ggaaaagccg atgcgctcgc agaaggcgtc gaccgcatcg
     2401 accaggcctt cgtcggccag caccgcaagc tggtcgcaga cggcgtcgat atagtcgtcc
     2461 gggcgacccg aatactcggg cggcaaggca tgcgcgccga ggaaggtggt caccacgcgt
     2521 accggcagat gctcgccgag ccggcgtgcg acgcgcagca tcttgcgctc gttctccagg
     2581 tccagcccgt agcccgattt gatctcgatg gtggttacgc cctcggcgat cagctgtctg
     2641 gcgcggcgca tgctcgcggt cagcagcgcc tcttcgctcg ccgcacgggt tgcgcccacg
     2701 gtagccatga tgccgccgcc cgcgcgcgcg atctcctcat agctcgcgcc gttcaggcgc
     2761 gcctcgaact ccatgctgcg gttgcccgca tggacgatgt gggtgtggca gtcgatcagg
     2821 cccggcgtga gccagcggcc ctggccgtca tgatgcttgc gcgcgcgctg tccgtcgggc
     2881 aggtcggcgc gccggcccag ccaggcgatg cggccgtcgc gcactgcgat cgcaccgtcc
     2941 tgcacttcgc cgtagcccgc gccctccatg gtggcgagat gcacattggt ccacagcgcg
     3001 tcccatgttt cattcatgcc tgccctcctt gtccgccgca tgtgcccgag caatccgggc
     3061 aatcagccgc gccgccacgc gcgcggtgcg ctggtcgatg tccctgtccg gattgagttc
     3121 ggcaatgtcg gccacgcgca gcttgccgga tgccacggcg cggtcgatca ccggttcgat
     3181 cacctccagc ggcacgccgc gcgcggccgg cgcactgacg ccgggtgcga cggccgccgg
     3241 cagcacgtcg agacagacgg tgaggtagag gtgatcgact ccgacgagga aggtatcgag
     3301 cgtgcgcagc acctgatcgc ggcgcgtgat gtccatatct tcatcccgca gccaggtcac
     3361 gccgagctgc cgcgcgcgtg caaacagcgc ctgcgtgttc gcgtagtcgc tcacgccgag
     3421 gcagcagtaa tgaaacggcc agccgcgcgc cgcgcaatcc tccgcgatct gccggaacgg
     3481 cgtgccggaa ctcggggcac tggccatgcg cagatcgaag tgcgcatcga ggttcaggat
     3541 gccgatgcgc ggcgtcgctt cggtggcggc cagatgtgtc gccaggcccg agaacgatgc
     3601 ccaggcgatc tcatgaccgc cgcccatgcc caaaggcaac gcgccggcat cgagcaagcc
     3661 ggcaatacgc agtgccagtt ccgcctgtgc ggcctccagt ccgtcgccgc catcgtccgg
     3721 cgtgcagatc acgtcgccgg cgtcgcgcag cgcgttgcag tcgcgcgccg gcaggttcga
     3781 cagcgtcgcg cgcaacgcgg cgggcccgcc acgcgcgccg gtgcggccgt ggttgcgggc
     3841 cacgccggca tcgcaggcga acccgatcag cgcgacgctg ccggccgcgt cggcggcctc
     3901 aaacggacgc acgatctggt gccagcgctg tccgagcgga ccttcttccg cgtcgacgcg
     3961 cccctgccag gcgcgcatgt cgggttgccg gatcatgtgg ttgcctatcc gatgtagaga
     4021 ccgttgaaca gatcgccgca gtcgcgactg agcgcgccgt cgagcacgat gccgcgcgcg
     4081 tgctcgatgt ccggcgcgaa caggcggtcc ttgtcgtaga acggcacatg ctcgcgcaga
     4141 tgcgcatgca cggcggcaag ccgcggcgac gtcgccagcg gccggtggaa atcgatgccc
     4201 tgcgcggcgg cgagcagttc gatcccgagt atggtcgccg tgttctcggc catctcgtgc
     4261 aggcgccgcg cggcgaaggt ggccatgctc acatggtctt cctggttcgc cgaggtgggc
     4321 aggctgtcga ccgaggccgg atgggcgagc gacttgtttt cggacgcgag cgcggcggcg
     4381 gtcacgtgcg cgatcatgaa gccggaattc aggccggatg aggcaaccag gaaaggcggc
     4441 aggccggaca gcgtggagtc gatcagcagc gcgatgcggc gctcggcgag cgcgccggtt
     4501 tcggcgatcg ccagcgccag cgtgtcggcg gcaaaggcga ccggctccgc atggaagttg
     4561 ccgccggaga tcacgtcgcc gctgtccgca tagaccagcg ggttgtcggt gaccgcgttg
     4621 gcttcagtca gcagcgtgcg cgcggcgtgg ttgatcacgt ccaggcaggc gcccatcacc
     4681 tgcggctggc agcgcaggct gtacgggtcc tgcacgcgtt cgtcgttgtc caggtgcgag
     4741 cggcggatct cgctgccggc cagcagttcg cggtagacgc gcgccagcgc gatctggccg
     4801 ggttgcccgc gcaccgcgtg gatgcgcgga tcgaacggcg cgtcgctgcc cttggcggca
     4861 tcgaccgaca gagcgccggc gaccgtgccg gcttccagca tgcgttcggc catgaacagg
     4921 ccgtcgagcg ccagcgcggt cgacacctgc gtgccgttga tcagggccag gccttccttc
     4981 ggcgcgagcc gcacggcctc gatgttcgcc tgcgcgagcg ccgcgcttgc ggggaccaca
     5041 ttcccgccta cccgcacctg tccttcgccc atcattgcca gcgtcatgtg cgcgagcggc
     5101 gcgaggtcgc ccgaggcgcc gactgatccc ttcgatggaa tgcagggcag gatgccggcg
     5161 ttgaacatcg cgatcattgt gtcgatcacc gatgcgcgga tgccggaata gccgcgcgcg
     5221 aggctgccga tcttggtcgc gagtatcagg cgcacgacgc ggtcgtcgag cagcgcgccg
     5281 gtgccgaccg agtgcgacag gatcaggttg cgttgcagcg cttccagttg cgcgtcaggg
     5341 atatgcgtct tcgccagctt gccgaagccg gtattgatgc cgtaggccgg ggcgccgcgt
     5401 tcgagaatcc tgcgtatcgt cgcggccgag gcatgaatgg cttcgtaggc cgaaggttcc
     5461 agcgcgaggg tgaccgggcc gcgccagatg cggcgaagca tgtccagcgt gagctggccg
     5521 ggaatgagct tggtttccat gtttgtcttg tccgtgtcct cgatgtacag ccgtccttac
     5581 ttcaccatcg gcagcttcag gccattgcgc tgtgcgcagg caacggccgc ctcgtacccg
     5641 gcgtccgcat gccgcatcac acccgatccg ctgtcgttga ccagcacgcg ccccaggcgc
     5701 cgcgcagcag cctcgctgcc gtcggccacg atcaccatgc cggcgtgctg cgaatagccc
     5761 atgccgacgc cgccgccatg atgcagagac acccaggtcg cgccgccggc ggtattgatc
     5821 agcgcattga gcaagggcca gtccgagacc gcgtcgctgc cgtcgcgcat gccctccgtc
     5881 tcgcggttcg ggctggcgac cgagccggtg tcgagatgat cccggccgat cacgatcggc
     5941 gccttcagtt caccattctt caccatctcg ttgaatgcaa ggccggcgcg atggcgttcg
     6001 cccagaccca gccagcagat gcgtgccggc aagccctgga acgcgatgcg ctcgcgcgcc
     6061 atgtccagcc agcgatgcac atgccggttc tctgggaaca gttccttgat cttcgcatcg
     6121 gtccggtaga tgtcttccgg gtcgccggac agcgcgaccc agcggaacgg acccttgccc
     6181 tcgcagaaca tcggccggat atacgcggga acgaagccgg gaaagtcgaa cgcatgctcc
     6241 acaccctggt cgagtgcgac ctgccggatg ttgttgccat agtccacggt cggaatgccc
     6301 atcgcgtgga aatccagcat cgcgcgcaca tgcaccgcgc aggaagcagc ggcttcggcg
     6361 ccgaggcgcg cgtgctgctg cggatcgttc tgcgcggcct tccattgttc gacgctccag
     6421 ccgcgcggca gatagccgtt gatcaggtcg tgggccgagg tctggtcggt gacaagatcg
     6481 gggcgcatgc cgccggcctg cgcgcgcttg accagttcgg gaaaaatctc ggcggcgttg
     6541 ccgagcagac cgatcgagac ggcctccttg cgcgcgcaat gctcgcgcac cagttccagt
     6601 gcatggtcca gatcgcgtgc ctgcttgtcc aggtagcgcg tgcgcagacg gaaatcgata
     6661 cggctctgct ggcattcgat attcagcgac accgcgccgg ccagcgtggc cgccagcggc
     6721 tgcgcgccgc ccatgccgcc c
//
LOCUS       DATLGF010000084        29170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_1922,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000084 DATLGF010000000
VERSION     DATLGF010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 29170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29170
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1922"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VM406_02445"
     CDS             complement(<1..239)
                     /locus_tag="VM406_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVL74851.1"
                     /translation="MPEFPIRYESIEGDGVRLHTLVAGDGPPVILLHGFPESAHSWRH
                     QVPALVAAGYSAWVPNLRGYPPSDVPAARGAYRLE"
     gene            407..1141
                     /locus_tag="VM406_02450"
     CDS             407..1141
                     /locus_tag="VM406_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL74852.1"
                     /translation="MPSPHAAPSSQEAVFILRNVSKTYTMGEVQVRALMEVELELYRG
                     EFVVLLGASGSGKSTLLNILGGLDTPSTGTVTYLDHNLTEADDNALTRFRREHVGFVF
                     QFYNLIPSLTALENVQLVTEISESPMDPTEALDMVGLADRMHHFPAQLSGGEQQRVAI
                     ARAIAKRPDLLLCDEPTGALDYRTGKLVLSVLDRINRELGTTVAVITHNAAIAGMADR
                     VIHISSGRIQEITRNPARVSIEEIAW"
     gene            1135..3498
                     /locus_tag="VM406_02455"
     CDS             1135..3498
                     /locus_tag="VM406_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL74853.1"
                     /translation="MVAALDRKLLRDMWRMRGQAVAIALVALCAIATFVMMRGAYEAL
                     METRAVYYERYRFADVFAHVKRAPDTLLAQVREIPGVNEVHGRVVFQASLDVPGLDEP
                     ATGRLVSIPERLDEGLNRVFLRAGRAPQAGNRNEIIVSESFAQANKLQPGDSLLAVIN
                     ARQERLHIVGIGLSPEYVSEIGEGVFPDNRRFGVMWMEHEALAAALDMRDAFNDLTLT
                     LAPHGSEAEVIARLDILLAPYGTLGAYGRDDQVSHRFISDEMAQTRVSATVIPSIFLG
                     VVAFLIHNVLLRLTTLQRAQIALLKSFGYSNFAVGAHYLKFALATTMTGGALGIAVGV
                     WMGEGLAIRYAEFFKFPTFAFPLSAFSIAVSLLIAAASAVFGAALAVRRILRLPPAEA
                     MRPESPARFRPGPLERMGLQRFIALPVRIILRNLERRPLKALMSVFGIALAVSLLVTG
                     QFAFDALSEMVRLQFRVVQRDDVMVGFNEVRDRAAVHELASLPGVLRVEPVRNVPIEL
                     RAGHRMHKTALMALGPDPQLRRVVDEHELAVALPDDGIVLNAMLAELLAVRRGDHVFV
                     SFLDGRRETLSARVAMIVEEPIGSFAYMDLAALARMRAEAETISGAFLAVDPAERATL
                     YRTLKEVPAISSVNLREATIDSFMATVAENMRINTIVLTVFACVIAVGIVYNSARIAL
                     SEHALELASLRILGFTKNEACALLLGQQALITLAALPLGCLLGYGFAALIAALLSQET
                     FRIPLVVSTETFVFSMSVVVAAAVGSGFLVWRRVQGLDLIEVLKTRE"
     gene            3500..4699
                     /locus_tag="VM406_02460"
     CDS             3500..4699
                     /locus_tag="VM406_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVL74854.1"
                     /translation="MNKRNNNHRFRRLAYWAIGLLLAAGVLWIIFGPETVSVETATVV
                     EGPMQVTVNNQGQVRMRDRYVVTAPVAAELQRIALRQGDAVRRGQTVALLDPVPLDLR
                     QRQETVARLESARAIAREAAARVQRANAETELATSELERTRRLVDDGFVSAQAAEKAA
                     IAARSARAELDAARLRHQAAQADVRAAEAALAAADAPPGRERQIPLTAPVDGYVVQLE
                     ERSGRTVAAGTPLVTIGDPSRYEIVVDVLSTDAVRIPEHAPMLLEGWGGGRTLRAQVR
                     LVEPVAFTKISALGVEEQRVNVVADPVDQLGPLGDGYRIEARIVIWAADQVLKVPGSS
                     LFRVGERWRVFVLREGRLREQEVEVGRRNQDEAQVLSGLQAGDVVVRFPSNDLRDGMR
                     AEPRTRT"
     gene            complement(4701..5345)
                     /locus_tag="VM406_02465"
     CDS             complement(4701..5345)
                     /locus_tag="VM406_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVL74855.1"
                     /translation="MLSSQIDHITVTAASLEEGAQYVIDALGIAPQPGGEHPRMGTHN
                     LLMRLGESTYLEVIAPNPDAPAPDRPRWFALDDAESVARPRLATWVARTDDIHTASVA
                     APEPLGKIEPMSRASLNWLISIPEDGALVLGGAAPALIEWQVAAHPASGMADLGCTLE
                     RLELFHPDAERVTRLLRGLGMEGRVHASALPAGSVPFLLAHVRTPRGLCSIGGT"
     gene            5449..6432
                     /locus_tag="VM406_02470"
     CDS             5449..6432
                     /locus_tag="VM406_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HVL74856.1"
                     /translation="MRAMLLDTPGRPLRAALVPVPDPGPHDVLLRVRACGVCRTDLHI
                     VDGDLAPHKQPVIPGHEIVGTVEATGAQVSGFAVGDRIGVPWLAGTCRHCAWCDDGHE
                     NLCDAARFTGYDRDGGYAQYTCADARYCFALPDNYDDLHAAPLLCAGLIGYRAYAMIG
                     NARRIGLYGFGAAAHILVQVARHEGREVYAFTRSADTRSQAFALSLGAAWAGDAGTMP
                     PEPLDAAILFAPVGALVPQALAATRKGGTVVCAGIHMSDIPAFPYSLLWGERCVRSVA
                     NLTHADGDAFFAIASRMTIRTSPHPFPLEDANAALAQLRAGAFDGAAVLVP"
     gene            complement(6793..7572)
                     /locus_tag="VM406_02475"
     CDS             complement(6793..7572)
                     /locus_tag="VM406_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HVL74857.1"
                     /translation="MAKLLAIDILNLVRRLYEANSEPDTAEKMEAVLHGAAAAVRRVL
                     EVHQPTHVLCALDHGGPSWRHAIHPAYRETHASMPDPLRETLPRLHALLAGLGVPVVS
                     VEGAEADDMIATAVRRWLAEGRGEAVIASTDRKLHPLVAEGALVWDAFRNEWHDSAWV
                     ERRYGVPPEMLADLLALVGNPAHGIPGIPKIGEKTAAKLLHAYRDLDGIMAGAGILLN
                     PLGQRLRAGRDDAYLSRELLRLKSDVRLGITWNALRFPAAR"
     gene            complement(7813..8289)
                     /locus_tag="VM406_02480"
     CDS             complement(7813..8289)
                     /locus_tag="VM406_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVL74858.1"
                     /translation="MEPQGAPIVTAQMLIRKPVEKVFDAFINPAVTTLFWFSKSSGPL
                     EAGREVLWEWEGLGASAVVRVQEIEPCLRILIEWDDPPCPVEWLFTPYEEQGTLVRIS
                     NWGFKGSADEVMTQALDAKGGFTMVLAGAKALLEHGIVLDLVGDQYPERVLRAFPA"
     gene            complement(8332..9636)
                     /locus_tag="VM406_02485"
     CDS             complement(8332..9636)
                     /locus_tag="VM406_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74859.1"
                     /translation="MRADTPPSALEQVRSFPLIEALFGRRARRFSLGASIPDGPLAYT
                     SRHEPVPLTEFEQMMVLTAAGGNTGWHFMITRNPRYAPHLANYSAAAGGRTFPSAAGF
                     HTSELFFTDDNGVYFFPTRDAPRLVERAPGAPVDPQAVVDAHRKRVRKLHDARLNIPP
                     AEPYMEGHNTWCANRPGSTLIIPVADIAQHAIAILCFLTQNGYCLDDDVNREPIPGLE
                     DLRQALKIENTFPLTYFDQYALTEATVEIGTSCFAGALMLQAMGLGGWMFDGIDRHVI
                     LGASGDPDVPGLGFRYDSDERWSIPNPTGLPGVFEGYCPPHFPDMRAAVEAFVQRKFG
                     PGGPFNPDTPGPWKESARVRASAEVHGDAFKECVGLQAQYVFDRFGKFPATVPSIFSL
                     MYLQAHHLDLEFYDHYFAPGAYLQTHAEHMARWHGAEPQPGQ"
     gene            complement(9921..11294)
                     /gene="radA"
                     /locus_tag="VM406_02490"
     CDS             complement(9921..11294)
                     /gene="radA"
                     /locus_tag="VM406_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HVL74860.1"
                     /translation="MAKQKTQYVCTECGGVTAKWAGQCPSCGQWNTLVESVIEPSGGN
                     RFSGQYQGLAQTSPVLSLADIEAIDVPRFTTGIDEFDRVLGGGLVAGGVVLIGGDPGI
                     GKSTLLLQALANMARVKKALYVSGEESGAQIALRAKRLALEAKDLKLQAEIQLEKILA
                     TLAEHKPEVAVIDSIQTIYSDALTSAPGSVAQVRECAAQLTRVAKQSGIAIIMVGHVT
                     KEGALAGPRVLEHIVDTVLYFEGDTHSSFRLVRAFKNRFGAVNELGVFAMTEKGLKGV
                     SNPSALFLSQHDNQVPGSCVMVTQEGTRPLLVEIQALVDTSHVPNARRLSVGLEQNRL
                     AMLLAVLHRHAGIAAFDQDVFINAVGGVKITEPAADLAVLLAIHSSMRNKPLPRGLVV
                     FGEIGLAGEIRPAPRGQERLREAAKLGFSQALIPKSNAPKQKIEGLNVIGVERIDEAL
                     NRLREVD"
     gene            complement(11287..12369)
                     /gene="alr"
                     /locus_tag="VM406_02495"
     CDS             complement(11287..12369)
                     /gene="alr"
                     /locus_tag="VM406_02495"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HVL74861.1"
                     /translation="MPRPLTATIHLSALRHNLALARRQAGNARVWAIVKANAYGHGLE
                     RAMRAFADADGLGLVEVDAAVRLRELGWRKPILLLEGFFDASDVPLLARYRIETAVHC
                     DEQIALLAEASADAAIEVHLKMNSGMNRLGFTPDAYRTAHAHLRANPAVAGITLMTHF
                     ANADDPGCAGLPLEAQLCRFNEGAAGLAGARSLSNSAAVLTRPDLAADWVRPGIMLYG
                     GTPGGRSAADFGLQPAMSLQAGIIGVQDVAAGAAVGYGSRFIADRPMRIGVIACGYAD
                     GYPRHAPAGTPVVVDGRRTGIVGRVSMDMITIDLTDIPGAGIGSTVELWGRQLPIDEV
                     AAAAGTIGYELMCALAPRVQVVEDNG"
     gene            complement(12461..13435)
                     /locus_tag="VM406_02500"
     CDS             complement(12461..13435)
                     /locus_tag="VM406_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HVL74862.1"
                     /translation="MRSSAHPAEPGRPPSHQSGFHQRWGHLRDPHVRTLAWLLDAPAL
                     LDPDAPEWAGRIASLPTADDATAAWLAALDRAPAPLHAALDMQPFTRLGRYAENLLGY
                     YLREHGHLVAHNLQVRAEKGGTIGEFDFILRADNGALMHWEFATKLYLLETEGAGHVA
                     DYFVGPNLADTLGAKIRKIMDHQLALSAHPAAQPVLPEPVAAAQALVKGWLFYHRATP
                     QVAAPAGIAHAHCRGFWCALDEFAGDAASRYAILPRLSWLAPARVPVEEGMDRDTLLR
                     ELAQRLSGDAAPVLVAILEPAGGDLLEVDRGFVVPDDWRERARRRLSG"
     gene            complement(13410..14225)
                     /locus_tag="VM406_02505"
     CDS             complement(13410..14225)
                     /locus_tag="VM406_02505"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HVL74863.1"
                     /translation="MGLGPQWTRRDLPRPAEVPGAAAAVSEEAVVAAMPQQEEAAVIV
                     DDFIDAFDAAPPAQPVLALAEDAGRAEEDAAIARMSWEELEQAIASCTRCGLCKGRTR
                     AVPGVGDRKARWMFIGEGPGREEDQRGEPFVGRAGQLLDNMLAAMKLARGQNAYIANI
                     VKCRPTDAAGKDRRPTAEEAAACLPYLLRQIALAQPDLVVALGKTSAVTLLGMDPETP
                     VASLRGRVHTYAARPMVVTYHPAYLLRAPEDKRKAWADLCLALSEYAKLGAPG"
     gene            complement(14264..14755)
                     /gene="rimI"
                     /locus_tag="VM406_02510"
     CDS             complement(14264..14755)
                     /gene="rimI"
                     /locus_tag="VM406_02510"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HVL74864.1"
                     /translation="MSASFRVVSQSPTPAQLQFVPMRADDLPEVLAIENDVYPYPWTR
                     GNFLDSLYSGYRAELLRDDAGMLVGYFLLMMAVDEAHLLNISVRRDLHGHGIGRFQLD
                     RVADIARASGMASVLLEVRPSNARAYAIYLKYGFAEIGRRRGYYPSAGNTREDAIVMR
                     YTL"
     gene            complement(14752..15441)
                     /gene="tsaB"
                     /locus_tag="VM406_02515"
     CDS             complement(14752..15441)
                     /gene="tsaB"
                     /locus_tag="VM406_02515"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HVL74865.1"
                     /translation="MPTILAIETSSELASAALLHNGQLLFREASGFQTHSQTLLPMVQ
                     AVLAEAGIALADVDAIGFGAGPGSFTGVRTACGVAQGLGFGAGRPLVPVVTLEAMAEA
                     CRSETGAADVLVLLDARMEEVYWAQYRWCDGWQTVIAPRLGPAAAVAAEGEVVACGNG
                     LAAYASAFAPMPFAARGLAHVMPHAKQIALLAARRFAAGVSVDPREASPLYLRDKVAL
                     TTSERNARAAA"
     gene            15523..15720
                     /locus_tag="VM406_02520"
     CDS             15523..15720
                     /locus_tag="VM406_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfB family protein"
                     /protein_id="HVL74866.1"
                     /translation="MDVNTIAHLATTLASTATNQAVTVAVAKKAQQVQASSAAAMVAA
                     LPPVTSANLPAHIGRHVDTTA"
     gene            15918..16325
                     /locus_tag="VM406_02525"
     CDS             15918..16325
                     /locus_tag="VM406_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HVL74867.1"
                     /translation="MPAASLTLEADNQTLVAEWLGEGLWVVACLCAAWCDTCRAYRPG
                     FDALAAAHPDKRFVWIDIEDQADLVGDIDVENFPTLLIQRGDAVSFFGTVLPDAKLAD
                     RLIQAQADKSEAQLRADAAGSLGEFSLKTRLAQ"
     gene            16464..17075
                     /locus_tag="VM406_02530"
     CDS             16464..17075
                     /locus_tag="VM406_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HVL74868.1"
                     /translation="MFDNFFQAFIILLLVTDPFGSVPLFASALAHVAPERRTKVIIRE
                     CAIAFVLLLLFMFFGRYFLAALQLSDIALRIGGGVILFLIALRMVFPQPGGVFGSDGS
                     ESGEPFIVPLAIPALAGPSALVTVLLFSSRNAVETLMNVGVLVLVALVWLAVLLGAER
                     MQQVMGPRIMTAVERLMGLILTAMAVEMLLAGISAYVKSLALA"
     gene            complement(17116..18144)
                     /locus_tag="VM406_02535"
     CDS             complement(17116..18144)
                     /locus_tag="VM406_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVL74869.1"
                     /translation="MYKTTTTIGFARKAVLAMAAAAAMLPLTTSAQSAPQEGLSSRPI
                     TLIVPFPPGGPLDSAARILAEAVRPTLGTVVVENRSGAGGNIGMEAVAKAAPDGHTIG
                     IGALSTHAINPWLYTRMPFDPVKDFAPITLITDLPNVLVLSTSFAEKNKINTVADFVT
                     YLKANPGKINYGSGGNGSGGHLAAELFKQMAGVFMVHIPYRGAAPAQTALLSGEVDVM
                     FDNLGSAAPRIRDGQIKALAVTTTKRSAMLPDVPTVAESGVAGFKDFHVSTWFGLFAP
                     AGVQPAIINRLNKEFTAAMRTPAVKERMSRIAAEPSPTTPAQFGDLVKRELARYEGVV
                     KRSGAKVD"
     gene            complement(18385..18759)
                     /locus_tag="VM406_02540"
     CDS             complement(18385..18759)
                     /locus_tag="VM406_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HVL74870.1"
                     /translation="MSLCSVDSAAFLLLHRESLLEEGGEAGGISEEKLDAALAYPLRE
                     ARLTIPDVAALAAAYAVGMLRYRPFANGNQRAALLALGLFLYSNDWRLEATQEEATAA
                     IRRLDCGEMNEDELANWVRKNL"
     gene            complement(18756..18980)
                     /locus_tag="VM406_02545"
     CDS             complement(18756..18980)
                     /locus_tag="VM406_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HVL74871.1"
                     /translation="MHALKLIRIGDAVGVVLPREALARLQRGTGEIVFLTESHHGLTL
                     TADDPVLQKQLDVGREFIGAYRGALKALQR"
     gene            complement(19290..21512)
                     /locus_tag="VM406_02550"
     CDS             complement(19290..21512)
                     /locus_tag="VM406_02550"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HVL74872.1"
                     /translation="MSNRTTCHGLQVATNLYQFIEEKVLPGTGIDSASFWKNFDAIAH
                     ELAPKNAALLAERDRLQLELDNWHRANPGPIRDIAAYRGFLESIGYLVPRPDDVKVTT
                     ANVDAELALQAGPQLVVPILNARYALNAANARWGSLYDALYGTDAIPETEGGEKVGPN
                     GEAYNPSRGAKVIAFARNFLDQSAPLAKGSHRDSTGYRVEGGKLVVSLQDGSSTGLAD
                     ENKFIGFQGDAANPSSVLLKNNGLHIDIQIDRNTAIGKSDAAGVADVVIEAALSTILD
                     LEDSIAAVDADDKVLAYSNWLGILKGSLVEEVSKGGKTFTRTLNKDRIYNAGAGAADA
                     QDGKVRLHGRSLLFLRNVGHLMTNPAILLKDGKEIPEGIMDAVITVTIALHDLKRAKD
                     QDAGNSRTGSVYIVKPKMHGPAEVAFASELFGRVEAMVGLAPNTVKLGIMDEERRTSV
                     NLSACLKEASGRVAFINTGFLDRTGDEMHTAMHAGPMLRKGDMKTSTWLQSYERSNVL
                     VGLSAGLSGRAQIGKGMWAMPDMMAAMMEQKIAHPKSGANTAWVPSPTAATLHALHYH
                     QVDVFAVQRELQKLDAKTEIERLLEGLLQVPVAAEPKWTPAEIQQELDNNAQGILGYV
                     VRWIDQGVGCSKVPDIHNVGLMEDRATLRISSQHIANWLLHGIVSEAQVTETLQRMAK
                     VVDGQNAGDPFYKPMAPDFESSYAYRAARDLVFKGLEQPSGYTEPLLHAWRLKVKASA
                     "
     gene            complement(21539..22297)
                     /locus_tag="VM406_02555"
     CDS             complement(21539..22297)
                     /locus_tag="VM406_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVL74873.1"
                     /translation="MSVVDILIVSCAAFLGGAINSVAGGGTFFSFPALLAAGVPPVTA
                     NASNSVAMWPASLSGAWAYRHELARYKRYLIPMGIVSFIGGVGGGLLLLSTRDATFAK
                     LIPWLLLFATILFAFSSQLGTLLRGAKAIKPGNTPGALAGQAVVSIYGGFFGAGMGIM
                     MLASLAMSGHDDVHEINAIKNLLSAVIYSVTVATFVIAGAVSWPHTIVMLATATLGGY
                     IGASVARKIPALWLRRFIIAVGLLLTAFYFHKTV"
     gene            complement(22363..23040)
                     /locus_tag="VM406_02560"
     CDS             complement(22363..23040)
                     /locus_tag="VM406_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HVL74874.1"
                     /translation="MSIQAITFDAYGTLFDVYSIGALAERLFPGNGKALAELWRDKQI
                     EYTRLRTMCAMYKPFWEVTQDALVFSCRKLKLDLTLDAQNALMGQYAKLEAFPENLEV
                     LQQLQSMGLKLAILSNGNPQMLDAAVEAAGMRGIFHHILSVDAVKKYKTAPEAYQLAP
                     DVFGVSARNILFVSSNCWDACGATWFGYTTFWVNRADAPLEELGVTPTATGPDMTALL
                     EYVREGK"
     gene            23153..24091
                     /locus_tag="VM406_02565"
     CDS             23153..24091
                     /locus_tag="VM406_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL74875.1"
                     /translation="MDHFKQISSFVEVATRGSLTAAARAEGVAPAMIGRRLDALEERL
                     GVKLLQRSTRKIMLTNEGAIFLEDCQRILADLQDAEAGVSERSARASGHLLVSAPAGF
                     GRQHVAPLIPSFLAQHRDVTVTLNLNDRVVDLIGEGIDVAIRIAALSDSNLIGVKLAD
                     NKRVVVASPAYLKRHGRPESLDDLARHNCLAISSEGSQRGWTFRHAGKNVTLRVSGNM
                     VCNDGAVLHEWALAGKGLAWRSMWEVGAAIEAGALVTVLDEFAAPGNDIYAVFAQRRH
                     LPLRIRAFVDFLRRTYAEPGYWRERLRPSPPADQNW"
     gene            complement(24079..25428)
                     /locus_tag="VM406_02570"
     CDS             complement(24079..25428)
                     /locus_tag="VM406_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HVL74876.1"
                     /translation="MIKLFKAVKASAAAPAQPQPTAASADLEQFVAGIARRASGLGKE
                     SAELNGLVEELAAAGVKQKETFAVLSQEIGAMARANEEIEARTRASAASVAKARTAVE
                     QVGQGVASVTDKLSDVAAAAHDITQVALQTRLVAFNASVEAKRAGEAGRGFGVVAEAV
                     KDLAAKVEHSSKLIMSTVEELEARISALAADIRSDAAGAGSQGFEAAVSEVERSVEAI
                     AAAARQNLQACTGVLGSVDGLSAQVGKTAQALESARTRTEGFLSLSESLIDLAAESGI
                     ETEDTPFIKASIDITARIGAMFEDAVREGRIGLEDLFDQNYRPVPGSDPEQFVTRFTD
                     FCDATLQDLLDSVLGWSPKVAFGVICDQRGYIPTHNRKYAKPMSKDKVWNQANCRNRR
                     FFIGRTEMAAIRNERPFLLQTYRRDMGGGQFVVMKNLSVPLFVAGRRWGVFRMGYQF"
     gene            complement(25614..25817)
                     /locus_tag="VM406_02575"
     CDS             complement(25614..25817)
                     /locus_tag="VM406_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HVL74877.1"
                     /translation="MATGTVKWFNDSKGFGFITPDDGGEDVFAHFSAIQSNGFKSLKE
                     NQRVSFEVTTGPKGKQASAIQPL"
     gene            26034..27749
                     /locus_tag="VM406_02580"
     CDS             26034..27749
                     /locus_tag="VM406_02580"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HVL74878.1"
                     /translation="MPASPLPPGQDFRVSRLRTGRRADAIKRSLREHFGFSDLRPGQR
                     EVIDSVLAGRHTLAIMPTGAGKSLCYQLPALEMPGTTVVVSPLIALMKDQSEKLLDAG
                     LPAEELNSTLNESEEEEALERIEDAGSEFIFATPERLTDPEFIELLQRSRVSLFVVDE
                     AHCISHWGHDFRPAYLQLGAVIAALGGPTVLALTATATDEVVRDITHQLGLPAMHVIN
                     TGIYRPNLNYQVIQVTDEDARLPTLIDLVASMEGSGIVYTATVKAVEEVFEALQQAGQ
                     NAISYHGQMGGALRAHNQDIFMDGTARVMVATNAFGMGIDKPDIRFVIHYQIPSSLQA
                     YYQESGRAGRDGLPARCVLMYYRKDRQVQQFFLARRYPGIDELAAVYKTLRDLSQRGA
                     DTGMRQVDATLEDVPSTRVQVALKLLKDGGLVEQDRNLKYRPVGRHVERRRLDALASQ
                     YRDKSARDHEALEKMVFYAQTGFCRWKVLVEHFGEHVPWSHCGHCDNCVDPPERHLAP
                     EHVREHVPEEAAPAGPGLSPGTAVRVPKYGEGRVREQAGEKVTVEFPDGAHRVFLASF
                     VKTAE"
     gene            27865..>29170
                     /locus_tag="VM406_02585"
     CDS             27865..>29170
                     /locus_tag="VM406_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HVL74879.1"
                     /translation="MSNSPWHHRPAEDALAAHDASAHGLSGAEARERLARHGPNRLPP
                     PKKRGPLLRFLAQFHNVLIYVLLAAALVTALLGHWIDSIVILGVVLANTVIGFFQEGK
                     AEAALEAIRSMLSPTAVVMRDGRRQELPAEELVPGDIVVLASGDKVPADLRLIEVRNL
                     RIEEAALTGESVPVEKHLLAVAENAPLGDRRCMAYSGTLVTFGQARGLVVATGEATEL
                     GRINRMLAQVQELATPLLRKMATFARWLTGAILVIASALFAFGLLLRGYSATEMFTAA
                     VGLAVAAIPEGLPAILTVTLAIGVQRMAAQRAIIRRLPAVETLGSVTVICSDKTGTLT
                     RNEMTVQRVVTAVRSFDVSGTGYAPHGGFEIEGKEFGPDQAPELHDIAMVALLCNDAV
                     LRQEEGRWILEGDPTEGALLTLGMKAGLDPHSAGETFPRVDVI"
BASE COUNT         4767 a         9763 c         9715 g         4925 t
ORIGIN      
        1 tattcgaggc ggtaggcgcc gcgcgccgcg ggcacgtccg acggcggata gccgcgcagg
       61 ttcggcaccc aggccgagta cccggccgca accagcgccg gcacctggtg gcgccaggaa
      121 tgcgcacttt cgggaaagcc gtgcagcagt atcactggcg gaccatcgcc ggcgaccagc
      181 gtgtgcaggc gcacgccgtc gccttctatg ctttcgtatc ggatcgggaa ttcgggcacg
      241 gatatctgtt ggtgaccgcc aggggacggc agcatgtgcc ggagccggag cgaaaggaga
      301 aacacgacac catcgcgcga agccttgccg cccggcttga cctgcatcaa tcccgtggcg
      361 gggtgccgcc ttctagaatc ggctggtctc cccaacggcc gttccgatgc cctcgccgca
      421 cgcagcccct tccagccagg aagccgtctt catcctccgc aacgtcagca agacctacac
      481 gatgggcgag gtacaggtgc gcgcgctgat ggaggtggag ctggaacttt accgcggaga
      541 attcgtcgtg ctgctgggcg cgtccggcag cggcaagtcc acgctgctca acatcctcgg
      601 ggggctcgat acgcccagca ccggcacggt cacctatctc gaccacaatc tcaccgaggc
      661 ggacgacaac gcgctcacac gctttcggcg cgagcacgtc ggattcgtgt tccagttcta
      721 caacctgatt cccagtctta ccgcgctgga aaacgtgcaa ctggtgaccg agatcagcga
      781 gagtccgatg gacccgaccg aagcgctcga catggtcggc cttgcagacc gcatgcacca
      841 tttccccgcg cagctgtccg gcggcgagca gcagcgggtg gcgatagcac gcgcgatcgc
      901 caagcggccc gacctgctgc tgtgcgacga acccaccggc gcgctcgact accgcaccgg
      961 caagctggtg ctatcggtgc tcgaccgaat caaccgcgaa ctgggcacga cggtcgccgt
     1021 gatcactcac aatgccgcga tcgcaggcat ggcggaccgc gtgatccaca tcagcagcgg
     1081 acgcatccag gagatcaccc gcaaccccgc ccgcgtctcg atcgaggaga tcgcgtggta
     1141 gccgcgctgg accgcaagct gctgcgcgac atgtggcgca tgcgcggaca ggcggtcgca
     1201 atcgcgctgg tcgcgctgtg cgcgatcgcc accttcgtga tgatgcgcgg ggcctatgaa
     1261 gcgctgatgg aaacgcgcgc cgtctactac gagcgctatc gtttcgccga tgtgttcgcg
     1321 cacgtgaagc gcgcgcccga caccctgctc gcgcaggtgc gagagatccc gggcgtcaat
     1381 gaggtgcacg ggcgagtcgt gttccaggca tcgctggacg tgcccggact ggacgaaccg
     1441 gctaccggcc ggctggtttc gattcccgag cggctggacg aagggctgaa ccgggtattc
     1501 ctgcgcgccg gccgcgcgcc gcaggccggc aaccgcaacg agatcatcgt cagcgaatcg
     1561 tttgcccagg caaacaagct gcagccgggc gacagcctgc tcgcggtcat caacgcgcgg
     1621 caggagcggc tgcacatcgt cggcatcggg ctgtcgccgg aatacgtatc ggagatcggc
     1681 gaaggcgtgt ttccggacaa ccgccgcttc ggcgtgatgt ggatggagca cgaggcgctc
     1741 gccgcggcgc tggacatgcg cgacgcgttc aacgatctga cgctcacgct cgcgccgcat
     1801 ggcagcgagg ccgaagtcat tgcccggctc gacatcctgc tcgcccccta tggcacgctc
     1861 ggcgcctacg gccgcgacga ccaggtctcg caccgcttca tcagcgacga gatggcgcag
     1921 acacgcgtga gcgcgactgt gattccgtcg atctttctcg gcgttgtcgc cttcctgatc
     1981 cacaacgtac tgctgcgcct gaccacgctg cagcgcgcgc agatcgcgct gctcaagagc
     2041 ttcggctaca gcaacttcgc ggtcggcgcg cattatctga aattcgcgct cgccacgacg
     2101 atgacaggcg gcgcgctcgg catcgcggtc ggcgtgtgga tgggcgaagg cctggcgatt
     2161 cgctatgcag agttcttcaa gtttccgacc ttcgcgtttc cgctgtcggc gttctcgatc
     2221 gccgtatcgc tgctgatcgc ggcagcctcg gcggtgttcg gcgccgcgct cgcggtgcgg
     2281 cggatcctgc ggctgccgcc ggccgaggcg atgcggccgg aatcgccggc gcgctttcgt
     2341 ccgggtccgc tcgaacgcat gggactgcag cgcttcattg cgctgccggt gcgcatcatc
     2401 ctgcgcaacc tggaacggcg cccgctgaag gcgctgatgt cggtgttcgg aatcgcgctg
     2461 gcggtgtcgc tgctggtgac cgggcagttc gcattcgacg cactcagcga gatggtgcgg
     2521 ctgcagttcc gtgtagtgca gcgcgacgac gtgatggtcg gcttcaacga ggtgcgcgac
     2581 cgggcggccg tgcatgaact ggccagcctt cccggcgtat tgcgcgtgga gccggtacgc
     2641 aatgtgccga tcgagctgcg cgccggacac cgcatgcaca agaccgcgct gatggcgctc
     2701 gggccggacc cgcagctgcg ccgggtggtc gacgagcacg agctggccgt cgcgctgccg
     2761 gacgacggca tcgtgctcaa tgcgatgctg gccgaactgc ttgcggtaag gcgcggcgac
     2821 catgtgttcg tgagcttcct ggacggccgc cgcgaaacgc tgtcggcgcg cgtggcgatg
     2881 atcgtcgagg aaccgatcgg cagcttcgcc tacatggacc tcgccgcgct ggcgcgcatg
     2941 cgcgccgaag ccgaaaccat ttccggcgcc tttctcgcgg tggaccctgc cgaacgcgcc
     3001 acgctctacc ggacgctgaa ggaagtaccg gcgatcagca gcgttaacct gcgcgaggcg
     3061 accatcgaca gcttcatggc caccgtcgcc gagaacatgc gcatcaacac gattgtgctg
     3121 actgtatttg cctgcgtgat tgcggtgggc atcgtctaca actcggcgcg cattgcgctg
     3181 tccgagcatg cgctggaact ggccagcctg cgcatcctcg gcttcacgaa gaacgaagcc
     3241 tgcgcgctgc tgctcggtca gcaggcgctg atcacacttg ccgcgctgcc gctcggctgc
     3301 ctgctcggct acggcttcgc cgcactgatc gcggcgctgc tcagtcagga aaccttccgc
     3361 atcccgctgg tcgtctccac agagaccttc gtgttctcga tgtccgtggt ggtggctgcg
     3421 gcggtgggct ccggatttct ggtctggcgc agggtgcaag ggctggacct gatcgaagta
     3481 ctgaagaccc gcgaatgaca tgaacaagcg caacaacaat catcgtttcc gccgcctcgc
     3541 ctactgggcg atcggcctgc tgctcgcggc cggtgtgctg tggatcatct tcgggccgga
     3601 gacggtctcg gtggagaccg caaccgtcgt tgaaggaccg atgcaggtca cggtgaacaa
     3661 ccagggccag gtgcgcatgc gcgaccgcta tgtcgtcacg gcaccggtgg cggccgaact
     3721 gcaacgcatc gcgctgcgcc agggcgacgc ggtgcgccgc gggcagacgg tcgcgctgct
     3781 cgatccggtg ccgctggacc tgcgtcagcg ccaggagacg gtggcgcgac tcgaatcggc
     3841 gcgcgcgatc gcacgcgaag cggccgcccg cgtgcagcgc gcgaacgctg aaacggaact
     3901 ggcaacaagc gaactcgagc gtacgcgcag gctggtcgac gacggtttcg tttccgcgca
     3961 ggccgcggag aaggctgcga ttgccgcccg ctcggcgcgc gcggaactgg acgccgcccg
     4021 cctgcgtcac caggccgcgc aggccgatgt gcgcgcggcc gaagcggcgc tggcggcggc
     4081 ggacgcaccg ccggggcgcg agcgccagat accgctcacc gcgccggtgg acggctacgt
     4141 cgtgcagctg gaggaacgta gcggccgcac ggtcgctgcc ggcacgccgc tggtgacgat
     4201 aggcgatccc tcgcgctacg agatcgtggt ggacgtgctg tccaccgatg cggtgcgcat
     4261 tcccgagcac gcaccgatgc tgctcgaagg ctggggcggc ggccggacgc tgcgtgcgca
     4321 ggtgcgcctg gtcgagccgg tcgcattcac gaagatttcc gcacttggcg tcgaggagca
     4381 gcgcgtcaac gtcgtggccg atcccgtcga tcagctgggt ccgctcggcg acggctaccg
     4441 tatcgaggcg cgcatcgtga tctgggccgc cgaccaggtg ctgaaggtgc cgggcagcag
     4501 tctgttccgc gtcggcgaac ggtggcgggt attcgtgctg cgcgaagggc gcttgcgcga
     4561 acaggaagtg gaagtcggcc ggcgcaacca ggacgaggct caggttctct ccggactgca
     4621 ggcgggcgac gtggtggtgc ggtttccgag caatgatctg cgcgatggga tgcgcgccga
     4681 gccgcgcacg cggacctgat tcaggtcccg cctatgctgc acaggccacg cggcgtcctg
     4741 acatgggcaa gcagaaaggg aacgcttccg gcaggcagcg cggacgcatg caccctgccc
     4801 tccatcccca gcccccgcag cagccgcgtc acgcgttccg catccggatg gaacagttca
     4861 agccgctcca gcgtgcagcc gagatcggcc atgccggatg ccggatgcgc cgccacctgc
     4921 cattcgatca gcgccggcgc cgcgccgccc agcaccaggg cgccgtcttc cggaatgctg
     4981 atcagccagt tcaacgacgc gcgcgacatc ggctcgatct tgccaagcgg ctcgggcgcg
     5041 gccacgctgg ctgtgtgaat gtcgtccgtg cgcgcaaccc aggtcgccag ccgcggccgt
     5101 gcgacggatt ctgcatcgtc cagcgcaaac cagcgcgggc ggtccggcgc cggcgcgtcc
     5161 ggatttggcg caatgacttc aaggtaagtc gactcgccca gtcgcatcag caggttgtgc
     5221 gtgcccatgc gcggatgctc gccgccgggc tgcggcgcga tgccaagcgc atcgatgacg
     5281 tactgcgcgc cttcctcgag cgaagcggca gtcacggtga tgtggtcgat ctggctggaa
     5341 agcaagcggc acctctggct gggattgaaa atcgctccgc cgaatggcgg ggcggcttct
     5401 attatggaat gaaacgctac gcacgcggag gaacccatgc cggcagtcat gcgcgcgatg
     5461 cttctcgaca cgcccggccg gccgctgcgt gccgcgctgg taccggtccc cgatcccggt
     5521 ccgcacgatg tgctgctgcg ggtgcgtgcc tgcggcgtct gccgcaccga tctgcacatc
     5581 gtcgatggcg atctcgcgcc gcacaagcaa ccggtgattc caggccacga aatcgtcggc
     5641 accgttgaag cgaccggcgc gcaggtctcg ggttttgcgg tcggcgaccg catcggcgtg
     5701 ccctggctcg ccggcacctg ccgccactgc gcatggtgcg acgacggaca cgagaatctg
     5761 tgcgacgccg cccgcttcac cgggtacgac cgcgacggcg gctacgcgca atacacctgt
     5821 gccgacgccc gctactgctt cgcgctgccg gataactatg acgacctgca cgcggctccg
     5881 ctgctgtgcg ccggcctgat cggctaccgc gcctacgcga tgatcggaaa cgcccggcgc
     5941 atcggcctct acggcttcgg cgcggccgcg cacattctcg tgcaggtcgc gcgccatgaa
     6001 ggcagggaag tctatgcgtt cacgcggtcc gcagacacgc gctcgcaggc gtttgcgctg
     6061 tcgctcggcg cggcatgggc gggcgacgcg ggcaccatgc cgccggagcc gctggatgcg
     6121 gcgatcctgt ttgcgccggt cggcgcgctg gtgccgcagg cgcttgccgc cacgcgcaag
     6181 ggcggcaccg tcgtctgcgc gggcatccac atgagcgaca tccccgcatt tccgtattcg
     6241 ctgctgtggg gcgagcgctg cgtgcgctcg gtcgccaatc tgacccacgc cgacggcgac
     6301 gcattcttcg cgattgcaag caggatgacg atacgcacat cgccacaccc tttcccgctg
     6361 gaagacgcga atgccgcgct cgcgcaactg cgcgccggcg cgttcgacgg cgccgcagtg
     6421 ctcgtgccct gaatgcgtgt tccgttgaag gaactcaaca agcgctcgac ttgggtcccg
     6481 ctgcaagcgc ggctggccca gactgtctga gccccgcctt gcacgcgggc cgcacacttt
     6541 ccgagcggag gccggtcatg gatgcgagca ggattgcctt aatcgctgtt ttggcgggtt
     6601 tgtttcacgg atgcgtggtg ctcgtcgacg agccgaggaa tccgccgttg gaggccccgc
     6661 caccgcccgc attgccgacg ccggagccgg cgcagtccgg tgctggcgca gcaccggccg
     6721 cgacgccgct cgtgcccgag caaaacgcca aacccggggg aatcagagca tactggcgtt
     6781 acttgcagcc gctcaacgag cagcaggaaa acgcaacgcg ttccaggtga tgcccaggcg
     6841 tacgtccgat ttcagccgca gcagctcgcg cgacaggtag gcatcgtcgc ggccggcgcg
     6901 cagtcgctgg ccgagcggat tgagcaggat accggcgccg gccatgatgc cgtccagatc
     6961 acggtacgca tgcagcagct tcgcggcggt tttctcgccg atcttcggta tgccgggaat
     7021 gccgtgtgcc ggattgccga ccagcgcgag caggtcggcc agcatttccg gcggtacgcc
     7081 atagcgccgc tcgacccacg cactgtcgtg ccattcgttc ctgaacgcat cccagaccag
     7141 cgcgccctcg gcgaccagcg ggtgcagctt ccggtcggtg gaggcgatca ccgcttcccc
     7201 gcgcccctcg gccagccagc ggcgcaccgc ggtggcaatc atgtcgtccg cctccgcgcc
     7261 ttcgacggag acgaccggca cgccgagacc ggccagcagt gcgtgcaggc gtggcagcgt
     7321 ctcccgcagc ggatccggca tcgacgcgtg cgtttcacga taggccgggt gaatcgcatg
     7381 gcgccatgac ggcccgccat gatcgagcgc gcacagcaca tgcgtcggct ggtggacctc
     7441 aagcacgcga cgcacggccg cagccgcgcc gtgcaggacg gcttccattt tctccgcggt
     7501 atccggctcg gaattcgctt cgtagaggcg acgaaccagg ttcaggatgt cgatggcgag
     7561 gagcttggcc attccgtcgt tttcagctga cagcgtgttg cgtcatccgc acaagcggct
     7621 cgacgcaaag gccgatgtct tgcggcaggc tcggaacgtg gcgtgtcagc atagggttcg
     7681 gggaaggtcc ggattcggag gcgatcgtac gggcggacgc gcaggctgcc gcgactttag
     7741 catgctgcca gtctggcaaa ccagcttgcg cgcgaacgcg gcgcctgcgg gactgcgttc
     7801 cggtcccgcc gctcaggccg gaaacgcccg cagcacgcgc tccgggtact ggtcgccgac
     7861 caggtcaagc acgatgccgt gttcgagcag agccttcgca ccggccagca ccatcgtgaa
     7921 gccgcccttg gcatcgagcg cctgcgtcat cacctcgtcc gcgcttccct tgaagcccca
     7981 gttcgaaatg cgcaccagcg tcccctgctc ctcgtacggc gtaaacagcc attcgaccgg
     8041 gcacggcgga tcgtcccatt cgatcaggat gcgcaggcag ggctcgattt cctgaacacg
     8101 caccacggcc gaggcgccca gcccctccca ctcccacagc acctccctgc cagcttcgag
     8161 cggaccgctg ctcttcgaga accaaaacag cgtggtcacg gcgggattga tgaaggcgtc
     8221 gaatactttc tcgacaggct tgcgtatcag catctgtgcg gtaacgatgg gcgcgccctg
     8281 cggttccatt cgagtctcct gtgtgtggct ggacacgcgc gccggacaac gttattgccc
     8341 cggctgcggc tcggctccgt gccagcgcgc catgtgctcc gcatgcgtct gcaggtaggc
     8401 tcccggcgcg aaatagtgat catagaactc cagatccaga tgatgcgcct gcagatacat
     8461 caatgaaaag atgctcggca ccgtggccgg gaatttgccg aagcggtcga aaacgtactg
     8521 cgcctgcagc cccacgcatt ccttgaacgc gtcgccgtgc acctcggcgc tcgcacgcac
     8581 gcgcgcactc tccttccacg gacccggcgt gtccggattg aacggcccgc cggggccgaa
     8641 cttgcgctgc acgaaagcct cgaccgccgc acgcatgtcc ggaaagtgcg gcgggcagta
     8701 gccctcgaat acgccgggca ggccggtcgg attggggatg gaccagcgtt cgtcgctgtc
     8761 gtagcgaaag ccgaggccgg gaacgtccgg gtcgccgctc gcgccgagga tcacgtggcg
     8821 gtcgatgccg tcgaacatcc agccgcccag gcccatcgcc tgcagcatca gcgcgccggc
     8881 aaagcagctg gtgccgatct cgacggtcgc ctcggtcagc gcgtattggt cgaaataggt
     8941 cagcgggaag gtgttttcga tcttcagcgc ctggcgcaag tcctccagcc cgggaatcgg
     9001 ttcgcggttg acgtcgtcgt ccaggcagta gccgttctgc gtgaggaagc agaggatcgc
     9061 gatcgcgtgc tgggcgatgt cggccaccgg aatgatcagc gtgctgccgg gccggttcgc
     9121 gcaccaggtg ttgtgcccct ccatgtacgg ctctgccggc gggatgttca gcctcgcgtc
     9181 gtgcagcttg cgcacgcgct ttctgtgcgc gtcgacgacc gcctgcggat cgaccggcgc
     9241 gcccggcgcg cgctcgacca gcctgggcgc atcgcgcgtg gggaagaagt acacgccgtt
     9301 atcgtcggtg aagaacagtt cgctggtgtg aaagccggcc gccgacggga aggtacgccc
     9361 gcccgccgcc gcagaatagt tggccaggtg cggcgcgtag cgcgggttgc gggtgatcat
     9421 gaaatgccag ccggtattgc cgcccgccgc ggtgagcacc atcatctgct cgaactcggt
     9481 cagcggcacc ggttcatggc gcgaggtgta ggccagcggg ccgtccggaa tcgacgcgcc
     9541 gagcgaaaac cgccgcgcgc gccggccgaa caaggcttcg atcaggggaa agctgcgcac
     9601 ctgctcgagc gcgctgggag gggtgtcggc acgcattgca agcctccttg ccatggcttc
     9661 acggtagcgg ccgggtggat cggcaggatg cgctccgccc ggtcggcggg cgtacgggtt
     9721 cgcgttgccg ggcggacggg cgggaccccg tctttctccc ggcgcgcgac gggcccataa
     9781 cgggtccgca agccagtcta gtccatgcgg catgcggatg cgggcggcgc cagcgcgcgt
     9841 atgcgggttt cctgccggcg tttgatgtac gcgaatagat cgcgcgaaat gacaccccgc
     9901 tggaagcggg gccggtgccg ctagtccact tcgcgcaggc ggttcagcgc ctcgtcgatg
     9961 cgttccacgc cgatcacgtt caggccctcg atcttctgct tcggcgcatt cgacttcggg
    10021 atcagcgcct gcgagaagcc gagcttggcc gcttcgcgca ggcgttcctg tccgcgcggc
    10081 gccgggcgga tctcgccggc cagtccgatc tcgccgaaca ccacgaggcc gcgcggcagc
    10141 ggcttgttgc gcatcgacga gtggatcgcg agcagcaccg caaggtcggc cgcgggctcg
    10201 gtgatcttca cgccgccgac cgcgttgatg aatacgtcct ggtcgaaggc tgcaatgccg
    10261 gcatgccggt gcagcaccgc cagcagcatc gccagccggt tctgctccag ccctaccgac
    10321 aggcggcgcg cattcggcac atgggaggtg tcgaccagcg cctggatctc gaccagcagc
    10381 gggcgcgtgc cttcctgggt caccatcacg caggagcccg gcacctggtt gtcgtgctgg
    10441 gagagaaaca gcgcggaagg attcgacacg cccttcaggc ccttttccgt catcgcgaac
    10501 acgccgagct cgttgaccgc gccgaaacgg ttcttgaacg cgcgaacgag gcggaagctc
    10561 gaatgggtat cgccctcgaa gtacagcacg gtgtcgacga tgtgctcgag cacgcgcggg
    10621 ccggcgagcg ccccctcctt cgtcacgtgg ccgaccatga tgatcgcgat gcccgactgc
    10681 ttggccacgc gcgtgagctg cgccgcgcac tcgcgcacct gcgccaccga gccgggagcg
    10741 gacgtcagcg catcggaata aatggtctgg atcgagtcga tcaccgccac ttccggcttg
    10801 tgctcggcca gcgtggcgag aatcttttca agctggatct cggcctgcag cttcaggtcc
    10861 ttcgcctcca gcgccaggcg tttggcgcgc agcgcaatct gcgcgccgga ttcctcgccg
    10921 ctcacgtaca gcgccttctt cacgcgggcc atgttggcca gcgcctgcag cagcaacgtc
    10981 gatttgccga tgccgggatc gccgccgatc agcacgacgc cacccgcgac cagcccaccg
    11041 ccgagcaccc ggtcgaactc gtcgatgccg gtggtgaagc gcggcacgtc gattgcctcg
    11101 atgtcggcca gcgagagcac cggcgaggtc tgggcgaggc cctggtactg gcccgaaaag
    11161 cggttgccgc cggacggctc gatcacgctt tcgaccagcg tgttccattg gccgcaggac
    11221 gggcactggc cggcccactt ggcggtcacg ccgccgcatt cggtgcaaac gtattgggtt
    11281 ttctgcttag ccattgtctt ccaccacctg cacgcgcggc gccagcgcgc acatcagttc
    11341 gtagcctatc gtcccggcgg ccgcggccac ttcgtcgatc ggcagttgcc ggccccacag
    11401 ttcgaccgtg ctgccgatgc cggcgcccgg gatatcggtg agatcgatcg tgatcatgtc
    11461 catcgacacg cgcccgacga tgccggttcg gcggccgtcg acgacgaccg gcgtaccggc
    11521 cggcgcatgg cgcggataac cgtccgcata cccgcaggca atgacgccga tgcgcatcgg
    11581 cctgtcggcg atgaagcggc tgccatagcc gaccgcggcg cccgccgcga cgtcctgcac
    11641 gccgatgatg cccgcctgca ggctcatcgc gggctgcagc ccgaaatccg ccgcgctcct
    11701 gccgcccggc gtgccgccat acagcatgat gccggggcgc acccagtccg ccgctaggtc
    11761 gggccgggtc agtaccgccg ccgagttcga caagctgcgg gcgccggcca gaccggcggc
    11821 gccttcgttg aagcggcaca gctgcgcttc cagcggcagg cccgcacagc cgggatcgtc
    11881 ggcattggcg aagtgggtca tcagcgtgat gccggcgacc gcggggttcg cgcgcaggtg
    11941 tgcatgcgcg gtgcgatagg cgtcgggcgt gaagccgagg cgattcatgc cgctgttcat
    12001 cttcaggtgc acctcgattg cggcgtcggc agaggcctct gcgagcagtg cgatctgctc
    12061 gtcgcaatgc acggcagtct ctatccggta gcgcgccagc aacggcacgt cgctcgcgtc
    12121 gaagaagcct tccagcagca ggataggctt gcgccagccg agttcgcgca ggcgcaccgc
    12181 cgcatcgact tcgaccagcc cgagcccgtc ggcatcggca aacgcgcgca tcgcgcgttc
    12241 cagcccgtgg ccataggcgt tggctttcac gatcgcccat acgcgggcgt tgccggcctg
    12301 ccggcgcgcg agcgcaaggt tgtggcgcag ggccgaaaga tggatggtgg cagtcagcgg
    12361 acggggcatg tcgggatgtt cggagagggt gttcgcattg ccgggcggac cttggcaacg
    12421 cgacggcaca tcggacgtga agcagggatt ctaccggagc tcacccgctc agacgccgcc
    12481 gtgcgcgctc gcgccagtcg tcgggcacga cgaagccgcg gtcgacctcg agtaggtcgc
    12541 cgccggccgg ctcgaggatg gcgaccagca ccggcgcggc gtcgccgctc agcctttgcg
    12601 caagctcgcg cagcagcgtg tcgcggtcca tgccctcttc caccggcacg cgcgccggcg
    12661 cgagccagga caggcgcggc aggatcgcat aacgcgatgc cgcgtcgccg gcaaactcgt
    12721 ccagcgcgca ccagaagccg cggcaatgcg cgtgcgcgat gccggcgggc gctgcgacct
    12781 gtggggttgc ccggtgatag aacagccagc ccttgaccag cgcctgcgct gcggcgaccg
    12841 gctccggcag caccggctgc gcggcgggat gggcggacag cgcgagctgg tggtccatga
    12901 tcttgcggat cttcgcgccc agcgtgtcgg ccaggttcgg cccgacgaag tagtcggcga
    12961 catggccggc gccctcggtt tcgagcagat aaagcttggt cgcgaactcc cagtgcatga
    13021 gcgcgccatt gtcggcgcgc agaatgaaat cgaactcgcc aatcgtgccg cccttctcgg
    13081 cacgcacctg caggttatgc gcgaccaggt ggccgtgctc gcgcaggtag tagccgagca
    13141 ggttctccgc atagcggccg agccgcgtga acggctgcat atccagggcc gcgtgcagcg
    13201 gcgccggcgc ccggtcgagt gcggccagcc atgcggcggt ggcgtcatcc gcggtcggca
    13261 ggctggcgat gcggcccgcc cattccggcg cgtcgggatc gagaagtgcg ggcgcgtcga
    13321 gcagccaggc gagcgtgcgc acgtgcggat cgcgcaggtg gccccagcgt tgatgaaatc
    13381 cgctctggtg gctgggcggc cggcccggct cagccgggtg cgccgagctt cgcatattcg
    13441 gacagggcca ggcacaggtc tgcccaggcc tttctcttgt cctcgggcgc gcgcagcagg
    13501 taggcgggat ggtaggtcac caccatcggc cgcgccgcgt aggtgtgcac acggccgcgc
    13561 aggctggcga ccggcgtctc cgggtccatg cccagcagcg tgacggccga ggtcttgccg
    13621 agcgcaacca ccaggtccgg ctgcgccagc gcgatctggc gcagcaggta gggcaggcag
    13681 gcggccgctt cttccgcggt cggacggcgg tccttgccgg cggcatcggt cgggcggcac
    13741 ttgacgatgt tcgcaatgta ggcgttctgg ccgcgcgcga gcttcatcgc ggcgagcatg
    13801 ttgtcgagca attgtccggc gcggccgacg aagggttcgc cgcgctgatc ctcttccctt
    13861 ccgggaccct cgccgatgaa catccagcgc gccttgcggt cgccgacgcc cgggacggcc
    13921 ctggtgcggc ccttgcacag gccgcaacgg gtgcagctgg cgatcgcctg ctcaagctcc
    13981 tcccagctca tgcgcgcgat cgcggcatcc tcctcggcgc ggccggcgtc ttcggcaagc
    14041 gccagcaccg gctgggccgg cggggcggcg tcaaacgcat cgatgaaatc gtcgacgatg
    14101 accgccgctt cttcctgctg cggcatggcg gcgaccaccg cctcttcgct gaccgccgca
    14161 gcggcgcccg gcacctcggc cggccgcggc agatcgcgcc gcgtccactg cggacccagt
    14221 cccatttctt cgagaaacgc cgcgcggcgt gcgggatcga tgctcacagc gtgtacctca
    14281 tcacgatcgc gtcttcgcgg gtgttgccgg cggacggata gtagccgcgg cgccgtccga
    14341 tctcggcaaa gccgtacttc agatagatcg cataggcacg ggcattggac gggcgcacct
    14401 cgagcagcac cgaagccatg ccggacgcgc gtgcgatatc ggccacgcgg tcgagctgga
    14461 agcggccgat accgtgtccg tgcagatctc ggcgcacgct gatgttgagc aggtgggcct
    14521 cgtcgacggc catcatcagc agaaagtagc cgaccagcat gccggcgtca tcgcgcagga
    14581 gttccgcgcg atagccgctg tagagggaat cgaggaaatt gccgcgcgtc caggggtatg
    14641 gatagacgtc gttctcgatg gccagcacct cgggcaggtc atccgcgcgc atcggcacga
    14701 actgcagttg cgccggcgtg ggcgactggg aaacaacgcg gaaactggcg ctcatgcggc
    14761 ggcccttgcg ttgcgttcgc tggtcgtcag cgcgaccttg tcgcgcaggt agagcgggct
    14821 ggcttcgcgc ggatccaccg acacacccgc cgcgaagcgc cgcgcggcga gcagcgctat
    14881 ctgcttcgca tgcggcatca catgtgcaag accgcgcgcg gcgaacggca tcggcgcaaa
    14941 ggcggatgcg taggcagcca ggccgttgcc gcatgcgacc acctcgcctt cggccgcaac
    15001 ggccgcggcc ggacccagac gcggggcaat caccgtctgc cagccgtcgc accagcggta
    15061 ctgcgcccag tagacctctt ccatgcgcgc gtcgagcaag accagtacgt cggccgcacc
    15121 ggtctcgctg cggcaggctt cggccatcgc ctccagcgtg accaccggca ccagcggacg
    15181 gccggctccg aagcccagcc cctgcgcgac gccgcaagcg gtgcgcaccc cggtaaacga
    15241 gccggggccg gcgccgaacc cgatcgcatc cacgtccgcc agcgcgatgc ctgcctcggc
    15301 cagcaccgcc tgcaccatcg gcagcagcgt ctgtgaatgg gtctggaagc cggatgcttc
    15361 gcgaaacagc agctgcccgt tgtggagcaa agcggcggaa gcgagttcgg aggaggtttc
    15421 gatggcgagg atggtcggca tggccggtat tttaccgcgc ggggcagccc taaatcccga
    15481 cgcggccctg ccgtaaacag ggattcacac caggagaatg tcatggacgt caacacgatt
    15541 gcgcatctgg ccaccaccct tgcctccacg gcaacgaacc aggcagttac ggtggccgtc
    15601 gcgaagaagg cgcagcaggt gcaggcctcg agcgccgcgg cgatggtcgc agcccttccc
    15661 ccggtcacga gcgcaaatct gccggcccat atcggccggc atgtggacac gactgcttaa
    15721 gcttcctgca tgggaagtgc ggttcaatta agcgataacc gttgttatgt cgctcccgcg
    15781 caggcgggag cgacatagct agggaattcg ctcagccgac ggcattccac tgccagacca
    15841 ccaccaccgc agtcccccgc acagtcccgc gccatcgcat acaatgacgg ccgctcaccc
    15901 gcaggtccaa agcaccgatg ccagccgcca gtctcaccct cgaagcagac aaccagaccc
    15961 tcgtcgccga atggctcggt gaggggctgt gggtggtcgc ctgcctgtgc gcggcctggt
    16021 gcgacacctg ccgcgcctac cgtcccggct ttgacgcgct ggccgccgcc catccggaca
    16081 agcgcttcgt ctggattgac atcgaggacc aggccgatct cgtcggcgac atcgacgtcg
    16141 agaactttcc taccctgctg atccagcgcg gcgacgcggt cagcttcttc ggcaccgtgc
    16201 tccccgacgc gaagctggct gatcgcctga tccaggcgca ggccgacaag agcgaagcgc
    16261 aactgcgggc cgacgcggcc ggaagcctcg gagaattcag cctgaagacg cgcttggcgc
    16321 agtaacccat gcgcggacct ctgcgccaaa tcccgccgtg ttcgccgtgt tcaagattcg
    16381 cgcgacttca tgatcctcgc gaacgccggc gccttgcccg catggcatcc tgatccttcc
    16441 cacactgcgg gagaagcgac gccatgttcg acaatttctt ccaggccttc atcatcctgc
    16501 tgctcgttac cgacccgttc ggcagcgtgc cgctgttcgc cagcgccctt gcccatgtgg
    16561 cgccggagcg gcgcaccaag gtcatcattc gcgaatgcgc gatcgcgttc gtgctgctcc
    16621 tgctgttcat gttcttcggc cgctacttcc tcgcggccct gcagctttcc gatattgcgc
    16681 tgcgcattgg cggcggggtg atcctgttcc tgatcgcgtt gcgcatggta tttccgcagc
    16741 ccggcggcgt attcggcagt gacggcagcg aaagcggcga gcccttcatc gtgccgctgg
    16801 cgattcctgc gctggccggc ccgtcggcgc tggtgacggt gctgctgttc tcgtcgcgca
    16861 atgcggtcga gacactgatg aatgtcggcg tgctggtgct ggtggcgctg gtatggctgg
    16921 cggtgctgct cggggcggag cggatgcagc aggtgatggg gccgcgcatc atgacggcgg
    16981 tcgaacgcct gatgggactg atactgaccg cgatggcggt ggagatgctg ctggcgggga
    17041 tcagtgccta cgtgaagtcg ctggcactgg catgaaaccg ccgcgccggg cggcgcggcg
    17101 gtgcatggaa caaggtcagt cgaccttcgc gccggagcgc ttgacgacac cctcgtagcg
    17161 cgcgagttcg cgcttgacca ggtcaccgaa ctgcgccggc gtggtcggcg acggttctgc
    17221 ggcgatacgg ctcatgcgct ccttcaccgc cggcgtgcgc attgcggcgg tgaattcctt
    17281 gttcaaccgg ttgatgatgg cgggctgcac gcctgccggc gcgaacaggc cgaaccaggt
    17341 gctgacatgg aagtccttga agccggccac accgctctcg gcgacggtcg gcacatccgg
    17401 cagcatcgcc gaacgcttgg tcgtggtgac tgccagtgcc ttgatctgac cgtcgcggat
    17461 gcgcggcgcg gccgaaccga ggttgtcgaa catgacgtcg acttcgccgc tcaacaacgc
    17521 cgtctgcgcc ggcgctgcgc cgcggtacgg aatgtggacc atgaacacgc cggccatctg
    17581 cttgaacagt tcggccgcca gatggccgcc gctgccgttg ccgccggagc cgtagttgat
    17641 cttgcccggg ttggccttca ggtaggtgac gaaatccgcc accgtgttga tcttgttctt
    17701 ctccgcgaag ctggtgctga gcaccagaac gttcggcagg tcggtgatca gcgtgatcgg
    17761 cgcgaaatcc ttgaccgggt cgaacggcat gcgggtgtac agccacggat tgatcgcgtg
    17821 ggtggacagc gcgccgatgc cgatcgtgtg gccgtccggc gccgccttcg caaccgcttc
    17881 catgccgatg ttgccgccgg cgccggagcg gttctcgacc actaccgtgc ccagcgtcgg
    17941 gcgaaccgcc tctgcgagga tacgggcggc cgaatcgagg ggaccaccgg gcgggaacgg
    18001 cacgatcagc gtgatcgggc gcgaagaaag accttcctgg ggggcggatt gcgcgctggt
    18061 ggtcagcggc agcatcgctg cggccgcggc catggcgagc acggccttgc gtgcgaatcc
    18121 gattgtcgtt gtggttttat acatggtgct atctactcct ttgaaaaagc accgcaacca
    18181 tagtcgcttg cgcgacgaac atcaagccgt acggcaactt ttttcgcgcc ctggccgaat
    18241 gagcgcgcga aacggcgccg tcggccgcct tacctggagg cagcccatcg catcgcaata
    18301 ggagcggagc tgcagcgcac ggacgcgcct gctgtgcccg gcacgtaacg tgcgatcagg
    18361 gcgcaggttg ttcaaggctt gtgatcagag gttcttgcgg acccagttgg cgagttcatc
    18421 ctcgttcatc tcgccgcagt ccagacgccg gatggcggcg gtggcttctt cctgcgtcgc
    18481 ttccagccgc cagtcgttgg agtaaagaaa gaggccgagc gcgagcaggg ccgcgcgctg
    18541 gttgccgttc gcaaacggcc ggtacctgag catgccgact gcgtacgcgg cggcaagcgc
    18601 ggccacgtca gggatcgtca gccgggcctc gcgcagcgga taggcaagcg cggcatcgag
    18661 tttttcctcg ctgatgccgc ccgcctcgcc cccctcttcg agcaggcttt cgcggtgcag
    18721 cagcagaaag gccgccgaat ccaccgaaca caggctcatc gctgcagcgc cttcagcgct
    18781 ccccgatacg cgccgatgaa ttcgcgcccc acgtcgagct gcttctgcag caccggatcg
    18841 tcggcagtga gcgtgagccc gtggtgggac tccgtcaaaa agacgatttc tccggtcccg
    18901 cgctggagcc tggcgagggc ttcgcgcggc aggacgacgc cgacggcatc gccgatccgt
    18961 atcagtttga gcgcatgcat aaacggtcct cacagacatg caggagcgcg gcgactttcg
    19021 gccagacaag tgtttcttcg tccggccggc tcggccggag ccgagagccg ccctctctac
    19081 ctgcgatcag gctgacgtat tcaagttccg tcgcctgcct gccggcgcgg cgcgcagcgg
    19141 cgccgtcgaa caggaaggtt cccttgctgc atgaggacga cacgtccgcg cgaatccggg
    19201 ccgatccggg cggagccggg caggttcggg cttgaaggga cggcaggcgc gacccgccgt
    19261 ccgcgaccgg ccgtccccga ccattagcct caggcgctcg ccttcacctt cagccgccag
    19321 gcatgcagca gcggctcggt atagccgctg ggctgctcca ggcccttgaa gaccaggtcg
    19381 cgcgcggcgc ggtacgcgta ggacgactcg aagtccggcg ccatcggctt gtagaacggg
    19441 tcgcccgcgt tctgtccgtc gaccaccttg gccatgcgct gcagcgtttc ggtgacctgt
    19501 gcttcgctga cgatcccgtg cagcagccag ttcgcaatgt gctggctgga gatgcgcagc
    19561 gtcgcgcggt cttccatcag cccgacgttg tggatgtccg ggaccttcga gcatcccacg
    19621 ccctggtcga tccagcgcac cacgtagccg aggatgccct gcgcattgtt gtccagctcc
    19681 tgctggatct cggccggcgt ccacttcggc tcggcggcaa ccggcacctg cagcagtcct
    19741 tcgagcagac gctcgatctc ggtcttcgcg tcgagcttct ggagctcacg ctggaccgcg
    19801 aacacgtcga cctgatggta gtgcagcgca tgcagcgtgg cggccgtcgg cgacggcacc
    19861 caggcggtgt tcgcgccgga cttcggatgc gcgatcttct gttccatcat cgccgccatc
    19921 atgtccggca tcgcccacat gcccttgccg atctgcgcgc gcccggacag gccggccgac
    19981 aggccgacca gcacgttgct gcgctcgtag gactgcagcc atgtggaggt cttcatgtcg
    20041 cccttgcgca gcatcgggcc cgcatgcatc gcggtgtgca tctcgtcgcc ggtgcgatcg
    20101 aggaagccgg tgttgatgaa ggccacgcga ccggaggcct ccttcaggca ggccgagagg
    20161 ttcacgctgg tgcgacgttc ctcgtccatg atgccgagct tgacggtatt cggcgcgaga
    20221 ccgaccatcg cctcgacgcg gccgaacagc tcgctggcga atgcgacttc ggccggcccg
    20281 tgcatcttcg gcttgacgat gtagaccgag ccggtgcgcg agttgccagc atcctgatcc
    20341 ttcgcgcgct tcaggtcgtg cagcgcgatc gtcacggtaa tcacggcgtc catgatccct
    20401 tcagggatct ccttgccgtc cttgagcagg atggccgggt tcgtcatcag gtggccgacg
    20461 ttgcgcagga acagcagcga gcggccgtgc aggcgcacct tgccgtcctg tgcgtcggcg
    20521 gcgccggcgc cggcgttgta gatgcggtcc ttgttcaatg tgcgggtgaa ggtcttgcca
    20581 cccttgctca cctcctcgac cagcgaaccc ttcaggatgc cgagccagtt gctgtaggcg
    20641 agcaccttgt cgtcggcgtc caccgcggca atcgagtcct cgaggtcgag aatcgtcgac
    20701 agcgccgctt cgatcaccac atcggccacg ccggccgcat cgctcttgcc gatcgcggtg
    20761 ttgcggtcga tctggatgtc gatgtgcagg ccgttgttct tcaggagcac cgacgacgga
    20821 ttggccgcat cgccctggaa gccgatgaac ttgttctcgt cggccaggcc ggtgctgctg
    20881 ccgtcctgca ggctgacgac cagcttgccg ccttcgacgc ggtagccggt cgaatcgcgg
    20941 tgcgaaccct tggccagcgg cgcggactgg tcgaggaagt tgcgcgcgaa cgcaatcacc
    21001 ttcgcgccgc ggctcgggtt gtaggcttcg ccgttcgggc cgaccttctc gccgccctcg
    21061 gtctcgggaa tcgcatcggt gccgtacagc gcgtcataca gcgaacccca gcgcgcattg
    21121 gcggcgttga gcgcatagcg cgcgttcagg atcggcacga ccagctgcgg gcccgcctgc
    21181 agcgcgagtt cggcatcgac gttggcggtc gtgaccttca cgtcgtccgg gcgcggcacg
    21241 agatagccga tcgattcgag gaagccgcgg taggcggcaa tgtcgcggat cggccccggg
    21301 ttggcgcggt gccagttgtc cagctcgagc tgcaggcgat cgcgttcggc cagcaaggca
    21361 gcgttcttcg gcgcgagctc atgcgcgatc gcgtcgaagt tcttccagaa gctggcgctg
    21421 tcgatgccgg tgccggggag caccttctct tcgatgaact ggtacaggtt ggtggcgact
    21481 tgcagcccgt ggcaggtggt gcggttcgac atggagaact ccttcgggtt ggtgtttttt
    21541 atacggtctt gtggaaatag aacgcggtca gcaagagtcc gaccgcaatg atgaagcggc
    21601 gcagccacag cgcggggatc ttgcgcgcga ccgaagcgcc gatgtacccg cccagggttg
    21661 ccgtggcgag catgacgatg gtgtgcggcc agctgaccgc gccggcgatc acgaaggtgg
    21721 cgaccgtcac gctgtagatc acggccgaca gcaggttctt gatcgcattg atctcgtgca
    21781 cgtcgtcgtg accggacatt gcgaggctgg cgagcatcat gattcccatg ccggcgccga
    21841 agaagccgcc gtagatcgac accaccgcct ggccggcgag cgcgcccggc gtgttgccgg
    21901 gcttgatggc cttcgcgccg cgcaggagcg tgccgagctg gctggagaac gcgaacagga
    21961 tcgttgcaaa cagcagcagc cagggaatca gcttcgcgaa ggttgcatcc cgggtcgaga
    22021 gcagcagcag cccgccgccg acgccgccga tgaacgagac gatgcccatc gggatcagat
    22081 aacgcttgta gcgcgcgagt tcatgccggt acgcccaggc gccggacagg ctggccggcc
    22141 acatcgccac cgagttgctc gcgttcgcgg tcaccggcgg cacgcccgcg gccagcaatg
    22201 ccgggaagga aaagaaggtg ccgcccccgg caaccgagtt gatcgcgccg ccgaggaagg
    22261 cggcgcagga gacgatcagg atgtcgacga cagacatggt tcagcgggtt ctgcagtgga
    22321 tggaacagct tttcgccggt gcgggtgcgg caatcagacg catcacttcc cctctcgcac
    22381 gtactcgagc aaggcggtca tgtccggacc ggtcgcggtc ggcgtcacgc cgagttcttc
    22441 cagcggcgcg tcggcgcggt tgacccagaa ggtggtgtag ccgaaccagg tcgcgccgca
    22501 ggcgtcccag cagttgctgg agacgaacag gatgttgcgc gccgacacgc cgaatacatc
    22561 cggcgcaagc tggtacgcct cgggtgcggt cttgtacttc ttcaccgcgt ccaccgacag
    22621 gatgtgatgg aagatcccgc gcatgccagc cgcttccacc gccgcatcca gcatctgcgg
    22681 attgccgttg gaaagaatgg ccagcttcag gcccatgctc tgcagctgct gcagcacctc
    22741 caggttctcc ggaaaggctt ccagcttcgc gtactgcccc atcagcgcgt tctgcgcatc
    22801 cagcgtcagg tccagcttga gcttcctgca cgagaacacc agcgcatcct gcgtcacttc
    22861 ccagaacggt ttgtacatcg cgcacatcgt gcgcaggcgc gtgtactcga tctgcttgtc
    22921 gcgccacagt tcggccagcg ccttgccgtt gccgggaaac aggcgttcgg ccagcgcgcc
    22981 gatcgagtac acgtcgaaca gcgtgccgta ggcgtcgaag gtaatggcct ggatgctcat
    23041 ctggggggag gtcaagtctt atacaagacc cgagtatatc gatgaaaaaa ggctggaatc
    23101 ctcacgttta gtcactttat tattgcgttt tagtttcaaa tcgaggcgcg ccatggacca
    23161 tttcaagcaa atttcttctt tcgtggaagt ggcaacgcgc ggcagcctga ccgccgccgc
    23221 ccgtgcggaa ggtgtcgcgc cggcgatgat agggcggcgc ctggatgcgc tggaggagcg
    23281 cctcggggtc aagctgctgc agcgctctac ccgcaagatc atgctgacca acgaaggggc
    23341 catcttcctc gaggactgcc agcgcatcct cgccgacctc caggacgcgg aagccggcgt
    23401 ttccgaacgc agcgcgcgcg ccagtggcca tctgctggtc tcggcgccgg ccggcttcgg
    23461 gcgccagcac gtggccccgc tgataccctc cttcctcgcc cagcaccgtg acgtcaccgt
    23521 cacgctgaac ctgaacgacc gggtggtgga cctgatcggc gaaggcatcg atgtggcgat
    23581 ccggatcgcc gcattgtcgg actcaaacct gatcggggtg aagctggccg acaacaagcg
    23641 cgtggtggtc gcctcccccg cctatctgaa gcggcacggc cggccggagt cgctggacga
    23701 cttggcccgc cacaactgcc tggcgatctc cagtgaaggc agccagcgcg gctggacctt
    23761 ccgccacgcc ggcaagaacg tgacgctgcg ggtgagcggg aacatggtgt gcaacgacgg
    23821 cgccgtgctg cacgaatggg cgctggccgg caagggcctg gcctggcgtt cgatgtggga
    23881 agtgggagcg gcgatcgaag cgggtgccct ggtgaccgtg ctcgacgagt tcgccgcgcc
    23941 cggcaacgac atctatgcgg tgttcgcgca gcgccgccac ctgccgctgc gcatccgcgc
    24001 cttcgtcgac ttccttcgcc gcacctatgc cgagcccggc tactggcgcg aacggctgcg
    24061 tccgtcgccg cctgccgatc agaactggta gcccatccgg aacacgcccc agcggcgccc
    24121 agcgacgaac agcggcaccg acaggttctt catcacgacg aactggccgc cgcccatgtc
    24181 gcgccggtag gtctgcagca ggaacggccg ttcgttcctg atggccgcca tttccgtgcg
    24241 gccgatgaag aagcggcgat tgcgacagtt cgcctggttc cagaccttgt cctttgacat
    24301 cggcttcgca tacttgcggt tgtgcgtggg gatgtagccg cgctggtcgc agatcacgcc
    24361 gaaggccact ttcggcgacc agccgagcac gctgtcgagc aggtcctgca gcgtcgcatc
    24421 gcagaaatcg gtgaagcgcg tgacgaactg ctccggatcg gagccgggta ccggacgata
    24481 gttctggtcg aacaggtctt ccaggccgat gcgcccttcc ctgaccgcat cctcgaacat
    24541 cgcgccaatg cgggcagtga tgtcgatcga agccttgatg aacggcgtgt cctcggtttc
    24601 gatgccgctt tccgcagcca ggtcgatcag cgactcggac agcgacagga agccttcggt
    24661 gcgggtacgc gcggattcga gcgcctgcgc cgtcttgccg acctgcgccg acaggccgtc
    24721 tacagatccg agcacgccgg tgcaggcctg caggttctgg cgcgccgccg cggcgatcgc
    24781 ctcgacgctg cgctcgacct cggataccgc cgcctcgaac ccctggctgc cggcgccggc
    24841 cgcgtccgaa cggatgtccg cggccagcgc ggaaatgcgc gcctccagtt cctcgacagt
    24901 gctcatgatg agcttggacg agtgctcgac cttggcggcc aggtccttga ccgcttcggc
    24961 caccacgccg aagccgcgtc cggcctcgcc cgcgcgcttg gcttcgaccg acgcgttgaa
    25021 tgcgaccagc cgcgtctgca gcgccacctg ggtgatgtcg tgggccgcgg cagccacgtc
    25081 ggagagcttg tcggtcaccg aggccacacc ctgccccacc tgctcgaccg cggtgcgcgc
    25141 cttcgccacc gaagccgcgc tggcacgcgt gcgcgcctcg atctcttcgt tggcgcgcgc
    25201 catcgccccg atctcctgcg acagcaccgc gaaggtttcc ttctgcttca cgccggcggc
    25261 agccagttcc tcgaccagtc cgttgagttc cgcggactcc ttgccaagcc cggacgcgcg
    25321 gcgcgcgatg ccggctacga actgttcgag atccgctgaa gccgccgtcg gttgtggctg
    25381 cgcgggcgcg gcggcactcg ccttgactgc cttgaaaagc ttgatcatat gtcccctgta
    25441 tgtgccggcg tggttcaccg attgttcttg tgcatcgtgc aggcgcttgc gcgctcgacg
    25501 atgctgttta cggcaggaac cgggtgtccc ttgagtcagg acgaggcgga caggtaaaaa
    25561 aaatgccgcc cggcgaaccg ggcggcattt ccgtcaaaac ctgtattgca gctttacagc
    25621 ggctggatcg ccgatgcttg cttgcccttc gggccggtgg tgacttcaaa cgacacgcgc
    25681 tggttttcct tgagggactt gaaaccgttg ctctggatcg ccgagaagtg cgcgaacacg
    25741 tcttcgccgc catcgtccgg ggtgatgaag ccaaaaccct tggagtcgtt gaaccacttg
    25801 acagtacctg ttgccattac catttcctta gaactacgtt taacacgggc ttgcgccccc
    25861 aacctttgaa gcaagaaaga aatgacagcg atactgcata cctagctcgg acttcaatcg
    25921 gtggtccatt atacgtcaag gattttgcac gggtactgga atcgaagcag tgctgcgtcg
    25981 atgcgacgcc ttcgcgtttg ccgctggcat gcttgctgca tcatctgacc cctatgcctg
    26041 catctcccct tccacccgga caagattttc gtgtctcgcg cctgcgcacc ggccgtcgtg
    26101 cggacgccat caagcgcagc ctgcgcgaac acttcggctt ctccgacctg cgccccggcc
    26161 agcgcgaggt gatcgacagc gtgcttgccg gacggcacac gctcgccatc atgcctaccg
    26221 gcgccggcaa atcgctctgc taccagctgc cggcgcttga aatgccgggc accaccgtgg
    26281 tcgtctcgcc gctgattgcg ctgatgaagg accagagcga gaagctgctg gatgccggac
    26341 tgccggcgga ggaactcaac agcacgctca atgaaagcga ggaggaagaa gcgctcgaac
    26401 gcatcgagga tgccggcagc gaattcatct tcgcgacacc ggagcgcctg accgatcccg
    26461 aattcatcga actgctacag cgcagccggg tcagcctgtt cgtcgtcgac gaggctcatt
    26521 gcatttccca ctgggggcat gatttccgcc cggcctacct gcagctgggc gcggtgatcg
    26581 ccgcgctggg cgggcccacc gtactggcgc tcaccgcgac cgcgactgat gaagtcgtgc
    26641 gcgacatcac gcatcagctg ggattgccgg cgatgcatgt aatcaacacg ggcatctacc
    26701 gtcccaacct gaactaccag gtcatccagg tcaccgacga ggacgcgcgg ctgccgacac
    26761 tgatcgacct cgtcgcctcg atggaagggt cgggcatcgt ctacaccgcc acggtgaagg
    26821 cggtggagga agtattcgag gccctgcagc aggccggcca gaacgcgatc agctaccacg
    26881 gccagatggg cggcgcactg cgcgcgcata accaggacat tttcatggac ggcaccgcgc
    26941 gcgtgatggt cgccaccaac gcgttcggca tgggcatcga caagccggac atccggttcg
    27001 tgatccatta ccagatcccg tccagccttc aagcctatta ccaggagtcc ggccgtgccg
    27061 ggcgcgacgg gctgcctgcc cgctgcgtgc tgatgtacta ccgcaaggat cgccaggtgc
    27121 agcagttctt cctggcgcgg cgctaccccg gcatcgacga gttggcggcg gtgtacaaga
    27181 ccctgcgcga tctgagccag cgcggcgcgg ataccggcat gcgccaggtt gacgcgacgc
    27241 tcgaggacgt cccttccacc cgcgtgcagg tcgcgttgaa gctgctcaag gacggcggat
    27301 tggtggaaca ggaccgcaat ctgaaatacc gtcccgtcgg caggcacgtc gagcgccgtc
    27361 gtctcgatgc gctcgcaagc cagtaccgcg acaagagtgc gcgcgaccac gaggccctgg
    27421 agaagatggt gttctacgcg cagaccggct tctgccgctg gaaggtgctg gtcgaacatt
    27481 tcggcgagca cgtgccgtgg tcgcactgcg ggcattgcga caattgcgtg gatccgcccg
    27541 aacgtcacct ggcccccgaa cacgtgcgcg agcatgtgcc cgaggaggcc gctcccgcgg
    27601 ggccgggatt gtcgcccggc actgcggtgc gcgtgccaaa gtatggcgaa ggacgcgtgc
    27661 gggaacaggc gggcgagaaa gtgacggtcg agttccctga tggcgcacac cgcgtattcc
    27721 ttgccagctt cgtcaagacc gcagagtgag cggggagcaa accggacttt tccgcccggc
    27781 tcaaaattga cctgcgtcaa atgaatcggc cggcgccatc ggtaccgtgc gacctttccc
    27841 cctgttcgcg agaaggaagc cgtcttgtcc aactccccct ggcaccaccg cccggccgag
    27901 gacgccctcg ccgcccacga cgccagcgct catggcctgt ccggcgccga ggcgcgcgag
    27961 cgcctcgcgc ggcacggccc gaaccgcctg ccgccgccaa aaaaacgggg accgctgctg
    28021 cgcttccttg cccagtttca caacgtgctg atctatgtgc tgctcgcggc ggcgctggtg
    28081 accgccctgc tcggccactg gatcgacagc atcgtcatcc tcggggtcgt gctcgccaac
    28141 accgtgatcg gctttttcca ggaaggcaag gcggaagcgg cgctggaagc gatccgcagc
    28201 atgctctcgc cgaccgccgt ggtcatgcgc gacggccggc gccaggaact cccggccgag
    28261 gaactggtgc ccggggatat cgtcgtcctg gcctccggcg acaaggttcc ggcggatctg
    28321 cggctgatcg aagtgcgcaa tctgcgcatc gaagaagcgg cgctgaccgg ggaatcggtg
    28381 ccggtggaaa agcacctgct ggcggtagcg gagaacgcac cgctcggcga ccgccgctgc
    28441 atggcctatt ccggcaccct ggtcaccttc ggccaggcgc gcgggctggt ggtcgcaaca
    28501 ggcgaagcca ccgagctcgg ccgcatcaat cgcatgctcg cgcaggtgca ggaactggcc
    28561 acgccgctgc tgcgcaagat ggcgaccttc gcgcgctggc tgaccggcgc catcctcgtc
    28621 atcgccagcg ccctgttcgc gttcgggctg ctgttgcgcg gctactcggc aaccgagatg
    28681 ttcaccgccg ccgtggggct ggcagtggct gcgattcccg aaggactgcc ggcgatcctg
    28741 acagtcaccc ttgcgatcgg cgtgcagcgc atggccgccc agcgcgcgat catccggcgg
    28801 ctgccggcgg tggaaaccct gggctcggtc acggtaattt gttcggacaa gaccggcacg
    28861 ctgacccgga acgagatgac ggttcagcgc gtcgtcacgg ccgtgcgcag cttcgacgtc
    28921 agcggcaccg gttatgcgcc gcacggggga ttcgagatcg agggcaagga attcggcccg
    28981 gatcaggcac ccgagctgca cgacatcgca atggttgcgc tgctctgcaa cgacgcggtc
    29041 ctgcgccagg aagaaggcag atggatcctg gaaggcgatc cgaccgaagg cgcactgctg
    29101 acgctcggca tgaaagccgg gctcgatccg cacagcgccg gagaaacctt tccgcgcgtc
    29161 gacgtgattc
//
LOCUS       DATLGF010000085         6632 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_1994,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000085 DATLGF010000000
VERSION     DATLGF010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6632
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1994"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /gene="atpA"
                     /locus_tag="VM406_02590"
     CDS             complement(<1..1388)
                     /gene="atpA"
                     /locus_tag="VM406_02590"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HVL74880.1"
                     /translation="MQLNPSEISELIKSRIQGLGETAEIQNEGTVISLTDGIARIHGL
                     SGAMQGEMLEFPGNTFGLALNLERDSVGAVILGDYEHISEGDTVKCTGRILEVPIGPE
                     LRGRVVNALGQPIDGKGPINTKLTAPIEKIAPGVIARQSVSQPMQTGLKSIDSMVPIG
                     RGQRELIIGDRQTGKSAVAVDAIINQKGQNMTCIYVAIGQKASTVKNIVRALEQHGAM
                     EYTIVVAANASESAAMQYVSAYSGCAMGEYFRDRGEDALIVYDDLSKQAVAYRQVSLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNPDYVEKFTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETSLFNAGIRPAINAGISVSRVGGAAQTKVIKGLSGG
                     IRTDLAQYRELAAFAQFASDLDEATRRQLDRGARVTELLKQPQYSPLPISLMAVSLFA
                     VNKGYFDDIEVK"
     gene            complement(1425..1958)
                     /locus_tag="VM406_02595"
     CDS             complement(1425..1958)
                     /locus_tag="VM406_02595"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HVL74881.1"
                     /translation="MAELATIARPYAEALFRVAKSGKLAAWTDLVSEMAAVAAHPDIK
                     SFASNPKLSDQQVVDTFLSLLKSNVTPEAKNFVVMLVENGRLSLLPEIGRQFFELKNA
                     DEGAADAEITSAFEMSDAQVKDLVATLENKFGRKLNPIVKVDSSLIGGVRVAVGDQVL
                     DTSVRAKLQQMYAALAS"
     gene            complement(1961..2431)
                     /locus_tag="VM406_02600"
     CDS             complement(1961..2431)
                     /locus_tag="VM406_02600"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HVL74882.1"
                     /translation="MNLNATLFAQFVVFFILAWFTMKFVWPPLMKALDERAERIATGL
                     AAAERGKAEMAAAEKRVQAELASARDESQKRVSDAEKRAQAIIEEAKKTAAEEAARII
                     AQAQADAEQQVARARDELRNQVAALAVSGAEQILKREVNAAAHADLLNQLKSGL"
     gene            complement(2504..2746)
                     /gene="atpE"
                     /locus_tag="VM406_02605"
     CDS             complement(2504..2746)
                     /gene="atpE"
                     /locus_tag="VM406_02605"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HVL74883.1"
                     /translation="MTDASFIALACAFIIGLGAIGASIGIAVMGGKFLEASARQPELM
                     NALQTRMFVLAGLIDAAFLIAVGIAMLFAFANPFAG"
     gene            complement(2830..3645)
                     /gene="atpB"
                     /locus_tag="VM406_02610"
     CDS             complement(2830..3645)
                     /gene="atpB"
                     /locus_tag="VM406_02610"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011655618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HVL74884.1"
                     /translation="MASANPTPSEYIVHHLGHLATSKQTAIVDFSVLNIDTLFWSIMM
                     GALTLFLLHRAAKRATSGVPGRFQAMVEMLVEMVENQSKSVVHGDRRFIAPLALTVFV
                     WIIFMNALDFLPVDMFAALFSVLGIDHILAYHRVVPTADLNGPLGMALGVLALMVYYS
                     IKIKGLGGWIHELFAAPFGPWMAPFNLLLNIVEYAAKTVSLGMRLFGNMYAGELVFLL
                     IALLGGTATALGFVGQVIAGSAWAIFHILIIVLQAFIFMMLTLVYLGQAHEGH"
     gene            complement(3647..3985)
                     /locus_tag="VM406_02615"
     CDS             complement(3647..3985)
                     /locus_tag="VM406_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HVL74885.1"
                     /translation="MLRIVLLQVAATLVTGLVAGLLAGKHALLSALLGGMCCVVPNAL
                     FALRLYMNAHKPGGASPATFFIGEFVKIVLTIALLAAVAVLYRDVNWLALIAAFIVAL
                     KSYIFILFRH"
     gene            complement(4360..4902)
                     /locus_tag="VM406_02620"
     CDS             complement(4360..4902)
                     /locus_tag="VM406_02620"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL74886.1"
                     /translation="MHARILILPGLYNSGEGHWQTLWEQTLPGAIRVQQREWNRPSRE
                     EWVLTLDAAVRASEGPVVLAAHSLGCALTAWWAQSAAQPELAKVRGALLVAPPDVERA
                     DFPRFVTGFAPMPAQRLPFATIVVASSDDPWCAFDRARRWSSTWGSDFVAAGPRGHIN
                     ADSALGDWAAGRDWLRQLAQ"
     gene            complement(4907..5788)
                     /locus_tag="VM406_02625"
     CDS             complement(4907..5788)
                     /locus_tag="VM406_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HVL74887.1"
                     /translation="MAIKKTKGLGRGLDALLGGSSDFSDAAPSVPGAPSALNVAQLQA
                     GKYQPRTRMDEGALQELANSIKAQGLMQPILVRPIAANGTDRYEIIAGERRFRAAQLA
                     GLTEVPVLVKDVDDQAAAAMALIENIQREDLNPLEEAQGIQRLISDFGFTHEQAAGAV
                     GRSRSAVSNLLRLLNLAKPVQTMLMAGDLDMGHARALLAVDSANQITLANQIVAKRMS
                     VREAEKLVAKSSAEQSSKPRAAKEKSRDIARLEEELSDALATAVAIKVGAKNRGQLIV
                     DFADLDALDALIAKLRA"
     gene            complement(5801..6571)
                     /locus_tag="VM406_02630"
     CDS             complement(5801..6571)
                     /locus_tag="VM406_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVL74888.1"
                     /translation="MAKIFCIANQKGGVGKTTTAVNLAAGLAQLDQRVLLVDLDPQGN
                     ATMGAGINKAELEASVYEVLLGLSDIAIARKRSESGRFDVLPANRELAGAEVEMVELE
                     NREKRLKDAIAAVAADYDFVLIDCPPALSMLTLNGLCAAHGVIIPMQCEYYALEGLSD
                     LVNTIKKVHAKLNPDLKIIGLLRVMFDPRMTLSQQVSAQLEQHFGDKVFKTIIPRNVR
                     LAEAPSYGMPGVSFDPASKGAQAYIAFGAEMVERIQNM"
BASE COUNT         1228 a         2019 c         2131 g         1254 t
ORIGIN      
        1 tgcttcacct cgatgtcgtc gaagtagccc ttgttcactg cgaacagcga cacggccatc
       61 agcgagatcg gcagcggcga gtattgcggc tgcttcagca gttcggtcac gcgcgcgccg
      121 cggtcgagct ggcggcgggt ggcttcgtcg aggtcagaag cgaactgcgc gaacgcggcc
      181 agttcacggt actgcgccag gtcggtacgg ataccgccgg acaggccctt gatgaccttg
      241 gtctgggcag caccaccgac gcgcgacacc gagatacctg cgttgatcgc gggacggata
      301 ccggcgttga acagcgaggt ttccaggaag atctgaccgt cggtgatcga aatcacgttg
      361 gtcggaacga aggcggacac gtcaccggct tgggtttcga tgatcggcag tgcggtcagc
      421 gaaccggtct tgcccttgac ttcgccgttg gtgaacttct cgacgtagtc ggggttcacg
      481 cgtgcggcgc gctcgagcag gcggctgtgc aggtagaaca cgtcgcccgg gtaggcttcg
      541 cggcccggcg gacggcgcag cagcagcgaa acctgacggt aggccacggc ctgcttggac
      601 aggtcgtcat agacgatcag cgcatcttcg ccgcggtcgc ggaagtattc gcccatcgcg
      661 cagccggagt aggccgacac gtactgcatc gcggcggatt cggaagcgtt ggcggcaacc
      721 acgatcgtgt actccatcgc gccatgctgt tccagcgcgc gcacgatgtt cttgaccgtc
      781 gatgccttct ggccgatcgc gacgtagata cacgtcatgt tctggccctt ctggttgatg
      841 atcgcatcaa ccgcaacggc cgacttgccg gtctggcggt cgccgatgat cagctcgcgc
      901 tggccacggc cgatcggcac catcgagtcg atcgacttca ggccggtctg catcggctgc
      961 gagacggact gacgagcaat gacgcccggg gcgatctttt cgatcggggc ggtcagcttg
     1021 gtattgatcg ggcccttgcc atcgatcggc tgacccaggg cgttcacgac gcggccgcgc
     1081 agttccgggc cgatcggcac ttccaggatg cggcccgtgc acttgacggt gtcgccttcg
     1141 gagatgtgtt cgtagtcgcc caggataacg gcgcccacgg aatcgcgctc gaggttcagc
     1201 gcgaggccga aggtgttgcc cgggaattcc agcatttcgc cttgcatcgc gccggacagg
     1261 ccgtggatgc gggcaatgcc gtcggtcagc gaaatgaccg tgccttcgtt ttgaatctca
     1321 gcggtctcgc caaggccttg aatccggctc ttgatcagtt cgctgatttc agaggggttg
     1381 agttgcatga taactcctaa attttttcac tctgcctgtg cggctcagga agccagcgcg
     1441 gcgtacatct gctgcagctt cgcgcgcacc gaggtatcga gtacctggtc gcccactgcc
     1501 acgcgcacac cgccgatcag cgaactgtcg accttgacga tcgggttgag cttgcgtccg
     1561 aacttgtttt ccagcgtagc gaccaggtct ttcacctgtg catcgctcat ctcgaacgcg
     1621 ctcgtgatct cggcatcggc tgcaccttca tccgcattct tcagttcgaa gaactgcctg
     1681 ccgatctccg gcagcaacga cagacgcccg ttctcgacca gcatcacgac gaagtttttg
     1741 gcttcggggg tgacgttgga cttcaacagg gacaaaaagg tgtcaacgac ttgctggtcc
     1801 gagagtttcg gattgctggc gaaggacttg atgtcgggat gagcggcaac tgccgccatt
     1861 tccgacacta ggtcggtcca ggcggcaagc ttgccggact tggctacacg gaacagcgcc
     1921 tctgcataag gacgagcgat cgttgcgagt tcggccatga ttacagcccg ctcttcagtt
     1981 ggttcaacag gtcggcgtgc gccgctgcat tcacctcacg cttcagaatc tgctcggcac
     2041 cggacacggc aagcgccgcg acctggttgc gcagttcatc gcgtgcacga gcgacttgct
     2101 gttcggcgtc ggcttgggcc tgcgcgatga tgcgcgcggc ttcttccgct gccgtcttct
     2161 tggcttcttc gatgatcgcc tgagcacgct tctcagcgtc gctgacgcgc ttctggcttt
     2221 cgtcgcgggc agacgccagc tcggcctgaa cacgcttttc ggctgcagcc atctcggcct
     2281 tgccgcgctc ggcagcggcc aggccggtcg cgatcctctc ggcgcgctcg tcgagcgctt
     2341 tcatcagcgg cggccacacg aacttcatcg tgaaccacgc gaggatgaag aagaccacga
     2401 actgtgcaaa cagggttgcg tttaagttca cggttttttc cttctcagag taacgaatgc
     2461 cgaacgttca ggttcggctt tgcaagtcag ccgggtagct gagttaccct gcgaacgggt
     2521 ttgcgaatgc aaacagcatg gcgataccga cggcgatcag gaacgctgcg tcgatcaggc
     2581 cggcgagaac gaacatacgg gtttgcagtg cgttcatgag ttcaggctga cgtgccgatg
     2641 cctcgaggaa cttgccgccc atcacggcga taccaatgga tgcaccgatg gcaccgaggc
     2701 cgatgataaa agcgcaagcc agagcgataa aagaagcgtc agtcatgatt ccctcaaaag
     2761 gttaagtaaa aaaacaaaaa agaaactgct ggtactgcca tcggttgcgg ccttccggcc
     2821 gcgaattcat caatgccctt cgtgtgcctg gccgaggtac accagggtca gcatcatgaa
     2881 gatgaaggcc tggagcacaa tgatcaggat gtggaagatc gcccatgcgg agcccgcaat
     2941 cacctggccc acaaagccaa gcgcggtggc ggtgccgccc aggagtgcga tgagcaggaa
     3001 caccagctca ccggcataca tgtttccgaa aagacgcatg ccgagcgata ccgtcttcgc
     3061 cgcgtattcg acaatgttga gaagcaggtt gaacggcgcc atccagggac cgaacggcgc
     3121 ggcgaaaagc tcgtggatcc agcctcccaa gcccttgatc ttgatgctgt agtagaccat
     3181 cagcgccagc acgccgaggg ccatgccgag cgggccgtta aggtcggccg tcgggaccac
     3241 gcgatgatag gcaagaatgt ggtcgatgcc gagaacggag aacagggcgg cgaacatgtc
     3301 gaccggcagg aagtcgagcg cgttcatgaa gatgatccag acgaacacgg tcagcgccag
     3361 cggagcgatg aagcggcggt cgccatgcac gacggacttg gactggtttt ccaccatttc
     3421 gaccagcatc tcgaccatgg cctggaagcg gcccggaacg ccggaggtgg cgcgcttggc
     3481 agcacggtgc aggaggaaca gggtcagcgc gcccatcatg atcgaccaga acagcgtgtc
     3541 gatgttgagc acggagaaat cgacgatggc tgtctgcttg ctggtggcaa ggtggcctag
     3601 gtggtgaacg atatactccg acggagtggg attcgctgat gccatgtcag tgcctaaaca
     3661 atatgaagat gtaacttttc agcgcgacga tgaaagcggc gatcagcgcc agccagttca
     3721 cgtcgcggta caacacggcc accgcggcta aaagcgcgat agtcagtacg atctttacga
     3781 attcaccgat gaagaatgtc gccggactgg cgcctcccgg cttatgcgca ttcatgtaaa
     3841 ggcgcaaagc gaacaacgcg ttcggcacca cgcagcacat gccccccagc agcgcggaca
     3901 acaatgcatg cttccccgcc aacaagccgg cgaccagacc ggtcaccaac gtggctgcaa
     3961 cttgcaggag gacaatgcgc agcatgaggg tcctgatgag atgcgatccg gcttcgctta
     4021 tggctcgcca ggcgccgcgc tgggtatgat gcttcgcgac aaaccacggg attatagggg
     4081 ttttccacat gcagcgtcaa accagcatct ctccagaact gtacaaatcc gtacggtaaa
     4141 actacggtac gagcgggcgt tcataccgaa gggtgatctt tcgcaaattt ctatgtccgc
     4201 gtcggaatca gcttgagact ccgcccttct gccggcaatt acttgtttgg taaatgcgaa
     4261 cggcgaccgg acacggtatc gatgtgttca gatggattgc attttctagg cgttgcgaac
     4321 ggcgggagat cgcgctgcga ggtgcgtgaa cgaaccgctt cactgcgcca gctgacgcag
     4381 ccagtcccgc cccgcagccc agtcgcccaa ggcgctgtcg gcgttgatgt ggccgcgtgg
     4441 gccggctgcg acgaagtcgc ttccccatgt gctggaccaa cgacgggctc ggtcgaacgc
     4501 acaccaggga tcgtcgctcg acgcgacaac gatggtcgca aacggcaagc gctgcgccgg
     4561 catcggcgcg aagcctgtga cgaagcgcgg gaaatcggcg cgctccacat cgggcggcgc
     4621 aaccagcagc gcgccgcgca ctttcgccag ttccggctgc gccgcggact gcgcccacca
     4681 ggcggtcagg gcgcagccca ggctgtgggc ggcgaggacc accgggccct cgctggcgcg
     4741 cacggccgcg tcgagcgtga gtacccactc ttcgcgggaa ggccgattcc actcgcgctg
     4801 ctgcacgcgt atcgcccccg gcagggtctg ttcccacagg gtctgccaat ggccttcgcc
     4861 ggagttgtac aggccgggca ggatcaggat ccgcgcgtgc atcgtgtcag gcgcgcagct
     4921 tggcgatcag cgcatcgagc gcgtcgagat cggcgaagtc gacgatcagc tggccgcggt
     4981 tcttcgcgcc gaccttgatc gccaccgcgg tggccagcgc atcggacagc tcttcctcca
     5041 gccgggcgat gtcgcgcgac ttctccttcg cggcgcgcgg tttgctgctc tgttccgcgc
     5101 tgctcttggc gaccagcttc tcggcttcgc gcaccgacat gcgcttggcg acgatctggt
     5161 tggccagcgt gatctggttc gcggaatcga ccgcgagcag cgcgcgggcg tggcccatgt
     5221 cgaggtcgcc ggccatcagc atcgtctgca ccggcttggc caggttcaac aggcgcagca
     5281 ggttcgacac agcgctgcgc gagcggccga ccgcgccggc ggcctgctcg tgggtgaagc
     5341 cgaaatcgga gataagccgc tggatgccct gggcttcttc gagcgggttc aggtcttcgc
     5401 gctggatgtt ttcgatcagc gccatcgcgg cggcggcctg gtcgtcgacg tccttgacca
     5461 gcaccggcac ctcggtcagg cccgccagct gggcggcgcg gaagcggcgt tcaccggcga
     5521 tgatttcgta gcggtcggta ccgttggccg cgatcgggcg caccaggata ggctgcatca
     5581 ggccctgggc cttgatcgaa ttggccagtt cctgcagcgc gccttcgtcc atccgggtgc
     5641 gcggctgata cttgccggcc tgaagctggg cgacgttgag cgcgctgggt gcgccgggca
     5701 ccgacggcgc cgcgtcggag aaatcggagg aaccgccgag cagcgcgtcg agaccgcggc
     5761 ccaggccctt ggttttcttg attgccatga tgagactgcc ttacatgttc tggatgcgtt
     5821 cgaccatttc ggcgccgaac gcgatgtacg cctgcgcgcc cttggaggcc ggatcgaagg
     5881 agacgcccgg catgccgtag gacggcgcct cggccaggcg cacgttgcgc gggatgatgg
     5941 tcttgaacac cttgtcgccg aaatgctgct cgagctgggc cgagacctgc tgcgacaacg
     6001 tcatgcgcgg atcgaacatc acgcgcaaga ggccgatgat cttcaagtcg gggttcagct
     6061 tcgcgtgcac cttcttgatc gtgttgacca ggtcggacag gccttcgagc gcgtagtact
     6121 cgcactgcat cggaatgatc acgccgtgcg ccgcgcacag gccgttgagc gtcagcatgg
     6181 acagcgccgg cgggcagtcg atcagcacga agtcgtagtc ggcggccacg gcggcgatcg
     6241 catccttcag gcgcttctcg cggttttcca gttcgaccat ctcgacttcg gcgccggcta
     6301 gctcgcggtt ggcggggagc acgtcgaagc ggccgctctc ggagcgtttg cgcgcgatgg
     6361 cgatgtcgga caggccgagc agcacttcat agaccgaggc ttccagttcg gccttgttga
     6421 tgcctgcgcc cattgtcgcg ttgccttgcg ggtcgaggtc gaccagcagt acgcgctgat
     6481 cgagctgcgc aagaccggcc gcgagattga ccgccgtcgt ggtcttgccg accccgccct
     6541 tctggttcgc gatgcaaaag atcttggcca tgggattcag gcccctccgc gggccggcag
     6601 gtggtgggat gtcgggcggg tcaccggctg aa
//
LOCUS       DATLGF010000086         6194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_144714, whole genome shotgun sequence.
ACCESSION   DATLGF010000086 DATLGF010000000
VERSION     DATLGF010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144714"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1232
                     /locus_tag="VM406_02635"
     CDS             171..1232
                     /locus_tag="VM406_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVL74889.1"
                     /translation="MNIWSYIIKRMLLMVPTLLGVIAITFAVIQFVPGGPVEQALMEL
                     RTAQVGGGEASGAGAGEYRGRQGLDAERVKEIQALYGFDKPPIERFWSMLKGFATFDL
                     GQSFYHHMDVWSLVVSKMPVSISLGMWTFFLTYFISLPLGVAKAVREGTRFDIGTSIA
                     VLIGYSIPGFVLGVFLLVMFAGGTFVQWFPLRGLTSDNWEELTLMGKVLDYLWHVTLP
                     VIASVAGSFAVMTMLTKNTFLEEIRKQYVLTARAKGLSEKSVLYKHVFRNALIPLVTG
                     FPAAFIGAFFAGSLLIETLFSLDGLGLLSYESVIRRDYPVVMGTLYLFTLIGLVVKLI
                     GDLCYVWVDPRVQFESLAR"
     gene            1243..2319
                     /locus_tag="VM406_02640"
     CDS             1243..2319
                     /locus_tag="VM406_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL74890.1"
                     /translation="MHSNEMMDNPVTAPLKLAPSVSPWKRVWLRFKQNRRGYWSLIIF
                     SILFGISLVAEVISNDKPLMVRYNGELLFPIVQNYPETRFGGDFDTPADYHDPFIKDQ
                     FERDGNWAIYPINRYSYDTLNYFAKEPNPSPPSSENWLGTDDRGRDVVARLLYGFRIS
                     VLFGLALTATGVVLGILAGAIQGYFAGKTDLLFQRFMEIWGSMPELYLLIIFASIFEP
                     SIGLLLILLSLFGWMGLADYVRADFLRNRNLEYVQAARAIGLSNSQIIWRHVLPNSLT
                     PVVTFLPFRMSAAILALTSLDFLGLGVPPPTPSLGELLAQGKNNLDAWWIALSTFFVL
                     TTMLLLLTNIGDALRNALDVRKKS"
     gene            2316..3938
                     /locus_tag="VM406_02645"
     CDS             2316..3938
                     /locus_tag="VM406_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL74891.1"
                     /translation="MSLIDVRNLRVEFGQNTVVQDVSFQIAPGEKFALVGESGSGKTV
                     TAYAILRLNQDAKYGGAILFEGQDLLKKTEAQMRGVRGKDVAMIFQEPMTALNPLYTI
                     GNQIAEVLMLHEGLNGKQAANRAVELLDKTGIPDPARRALAFPHQLSGGQRQRAMIAM
                     ALACRPKLLIADEPTTALDVTIQVQILELLNELQREHDMAVLMITHDLNLVRHFADRV
                     GVMEKGRLIESAMTDMLFANPREAYTKKLLGSRPRRVVDEVDPGQMPMLACRDLSCAF
                     PVKKGWFRKEKFVAVEKTALELRPRETLGIVGESGSGKSTLGMALLRLSIAQVDGEIS
                     FIGHPISAMSRNELRPLRAKMQVVFQDPFASLSPRRTIEQIVGEGLELHKPELGRDGI
                     RNAVGEALSEVGLAPEMMTRYPHEFSGGQRQRIAIARALVLKPELILLDEPTSSLDVS
                     VQQQVLLLLAELQRKYGIAYLFISHDLAVIRAMAHRVLVMKDGKVVESGPAQEVLSNP
                     REAYTQRLLAAATYSALNRQAVESAAVTGAEG"
     gene            complement(4055..5518)
                     /locus_tag="VM406_02650"
     CDS             complement(4055..5518)
                     /locus_tag="VM406_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HVL74892.1"
                     /translation="MTGIIGILLALILLIYLAYRGFSVLLLAPILALLAVLFAPDSPL
                     LASYTQVFMRALGDFVVLYFPLFLLGAVFGKLMEVSGCAHALARWIVGRLGERRAILA
                     IVLACAILTYGGVSLFVVAFAVYPIAAALFRQANVPKRLIPGTIALGAFTFTMTALPG
                     TPAIQNAIPMPFFGTTPFAAPGIGIIAAAIMFGVGMAWLAYRARRAAQAGEGYGESDA
                     PQAGMTQQPDKALRPLAAGENYDLAEVGGSTPAAEEPGAFVAVLPIVLVIAANYLFSA
                     IIWPALDTAYLALPLYGETDLQQVLGVWSIICALTLSIVVLLALNLRRIPSVKAALDD
                     GAAASVLPIFNTASLVGFGAVIASLGAFALIRDGVLGIAPGNPLISLAVAVNILAGIT
                     GSASGGMSIALQTLGDSYLAMGQEAGIAPALLHRVTAVATGGLDSLPHNGAVITLLSI
                     CGLTHRKAYFDIFVVAVLGPWLALIAIVAIGTFWGAF"
     gene            complement(5701..>6194)
                     /locus_tag="VM406_02655"
     CDS             complement(5701..>6194)
                     /locus_tag="VM406_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HVL74893.1"
                     /translation="VILVFGNLLSGFGIAAYEDTVAAYIALVFFLPLLVGSGGNAGSQ
                     AATLMVRALATGDVHMRDWLKMLGREFTVAGLLGLTMALAISGIGVARGGTDIAIVVA
                     TSMILIVVMGSLIGMSLPFILARFRLDPATASAPLVTSIADAVGVLIYLSLAFMLLGD
                     PPV"
BASE COUNT         1126 a         1965 c         2073 g         1030 t
ORIGIN      
        1 ggatggcgca gaggatggcg ttcggacggg cgtcgccgga agtcggagtc tgcaccccct
       61 ccctggccct cccctggaag ggggagggga tctgtcccgc cggagcccga tgcttcatcc
      121 cttggcaggc cgataggcga aggccgcgat aaacgtacag aaagctcacc atgaacatct
      181 ggtcctacat catcaagcgc atgctgctga tggtccccac cttgctcggg gtgatcgcga
      241 tcaccttcgc ggtgatccag ttcgtccccg gcgggccggt cgagcaggcg ctgatggagt
      301 tgcgcaccgc ccaggtcggc ggcggcgaag cgtccggcgc cggggcaggg gagtatcgcg
      361 gccggcaggg gctggatgcc gagcgcgtga aggaaatcca ggcgctgtac ggtttcgaca
      421 agccgccgat cgaacgcttc tggagcatgc tgaagggctt cgccaccttc gatctcggcc
      481 agagcttcta tcaccacatg gacgtctggt cgctcgtggt gtcgaagatg ccggtctcga
      541 tcagcctggg catgtggacc ttcttcctca cctacttcat ctcgctgccg ctgggcgtgg
      601 ccaaggcggt gcgcgagggc acgcgcttcg atatcggcac cagcatcgcg gtgctgatcg
      661 gctattcgat tcccggcttc gtgctgggcg tgttcctgct ggtgatgttt gccggcggca
      721 cgttcgtcca gtggttcccg ctgcgcggac tgacctcgga caactgggag gaactgacgc
      781 tgatgggcaa ggtgctcgac tacctgtggc acgtcacgct gccggtgatc gcctcggtcg
      841 ccggttcgtt cgcggtgatg acgatgctga ccaagaacac cttcctcgaa gagatccgca
      901 agcaatacgt gctgaccgcc cgcgcaaagg gcctgtcgga aaaatcggtg ctctacaagc
      961 acgtgttccg caatgcgctg attccgctgg tcaccggttt tccggcggcc ttcatcggcg
     1021 ccttcttcgc cggcagcctg ctgatcgaga ccctgttctc gctcgacggg ctgggcctgc
     1081 tgtcctatga atcggtaatc cggcgcgact atccggtcgt gatgggcacg ctgtatctgt
     1141 ttacgctgat cggcctggtg gtcaagctga tcggcgacct ctgctacgtg tgggtcgatc
     1201 cgcgcgtgca gttcgaaagc ctggcgcggt gatgcgatga cgatgcattc gaatgaaatg
     1261 atggacaacc cggtcacggc gccgctgaag ctggcgccgt cggtgtcgcc gtggaagcgg
     1321 gtgtggctgc gcttcaagca aaaccggcgc ggctactgga gcctgatcat cttctcgatc
     1381 ctgttcggca tcagcctggt cgccgaggtg atctcgaacg acaagccgct gatggtgcgc
     1441 tacaacggcg aactgctgtt cccgatcgtg cagaactatc cggaaacccg cttcggtggc
     1501 gacttcgata cgcctgccga ttaccacgat cctttcatca aggaccagtt cgagcgcgac
     1561 ggcaactggg cgatctatcc gatcaaccgc tatagctacg acacgctgaa ctacttcgcg
     1621 aaggagccga atccgtcgcc gccttcgtcg gagaactggc tcggcaccga cgatcgcggc
     1681 cgcgacgtgg tggcgcgcct gctgtacggc ttccggatct cggtgctgtt cggcctggcg
     1741 ctgaccgcga ccggcgtggt cctcggcatc ctcgcggggg cgatccaggg ttacttcgca
     1801 ggcaagaccg acctgctgtt ccagcgcttc atggagatct ggggctcgat gccggagctc
     1861 tacctgctga tcatcttcgc gtcgatcttc gagcccagca taggcctgct gctgatcctg
     1921 ctgtcgctgt tcggctggat gggtctggcc gactatgtgc gcgcggactt cctgcgcaac
     1981 cgcaacctcg aatacgtgca ggccgcgcgc gcgatcggcc tgtcgaactc ccagatcatc
     2041 tggcgtcatg tgctgccgaa cagcctgacc ccggtggtga ccttcctgcc gttccggatg
     2101 agcgccgcga tcctcgcgct gaccagcctg gactttctcg gcctcggcgt accgccgccc
     2161 acgcccagcc tgggcgagct gctggcccag ggcaagaaca atctggacgc gtggtggatc
     2221 gcgctgtcga ccttcttcgt gctgaccacg atgctgctgt tgttgaccaa tatcggcgat
     2281 gccctgcgca acgcgctcga cgtgaggaaa aagtcatgag cctgatcgac gtacggaacc
     2341 tgcgcgtcga gttcggccag aacacggtgg tgcaggatgt gagcttccag atcgcgcccg
     2401 gcgagaaatt cgcgctggtc ggcgaatccg gctcgggcaa gacggtcact gcgtatgcga
     2461 tcctgcgcct gaaccaggat gcgaagtacg gtggcgctat cctgttcgaa ggccaggacc
     2521 tgctgaagaa gaccgaggcg cagatgcgcg gcgtgcgcgg caaggatgtc gcgatgatct
     2581 tccaggagcc gatgacggcc ctgaacccgc tgtacacgat cggcaaccag attgccgagg
     2641 tgctgatgct gcatgaaggg ctcaacggca agcaggcggc caaccgcgcg gtggaactgc
     2701 tcgacaagac cggcattccc gatccggcgc ggcgcgcgct cgcgtttccg caccagctct
     2761 ccggcggcca gcgccagcgc gcgatgatcg cgatggcgct ggcctgccgt cccaagctcc
     2821 tgatcgccga cgaaccgacc actgcgctgg acgtgacgat ccaggtgcag atcctggagc
     2881 tgctcaacga attgcagcgc gagcacgaca tggcggtgct gatgatcacg cacgacctca
     2941 acctggtgcg gcatttcgcc gatcgcgtcg gcgtgatgga gaaggggcgg ctgatcgagt
     3001 cggcaatgac cgatatgctg ttcgccaacc cgcgcgaggc gtatacgaag aagctgcttg
     3061 gcagccgccc gcgccgcgtc gtcgacgagg tcgatcccgg ccagatgccg atgctggcct
     3121 gccgcgacct cagctgtgca ttcccggtga agaagggctg gttcaggaag gaaaaattcg
     3181 tcgcggtgga aaagaccgcg ctcgaactgc gtccgcgcga gacgctgggg atcgtcggcg
     3241 agtcgggctc gggcaagtcc accctgggga tggcactgct gcggctgtca atcgcccagg
     3301 tcgacggcga gatttcgttc atcggccatc cgatcagtgc gatgtcgcgc aacgaactgc
     3361 gtccgctgcg cgcgaagatg caggtggtgt tccaggatcc gttcgcctcg ctttcaccgc
     3421 gccgcaccat cgagcagatc gtcggcgaag gcctggaact gcacaagccc gaactcgggc
     3481 gcgacggcat ccgcaacgcg gtcggcgaag cgctgtccga agtcggcctt gcgccggaga
     3541 tgatgacgcg ctacccgcac gaattctccg gcgggcagcg ccagcgcatt gcgattgcgc
     3601 gcgcgctggt gctcaagccg gagctgatcc tgcttgacga gccgacttcc tcgctggacg
     3661 tctcggtgca gcagcaggtg ttgctgctgc tggccgagct gcagcgcaag tacggcatcg
     3721 cctacctgtt catcagccac gacctggcgg tgatccgcgc gatggcgcac cgggtgctgg
     3781 tgatgaagga cggcaaggtg gtcgagagcg ggccggcgca ggaggtgctg tcgaacccga
     3841 gggaggccta cacgcagcgg ctgctggcgg cggccacgta ttcggcgctg aatcgccagg
     3901 cggtggaatc ggctgcggtg accggtgcgg agggatgaga tccctcggct ttgcctcgcg
     3961 atgacagctt tgctgtcgtt acgagcgcag cgagaacctc acccgcatga tccgccgcgc
     4021 aatggggggc cggcgcagaa ccgatgccca gccttcagaa cgctccccag aacgtgccga
     4081 ttgcgacgat cgcgatcaac gccagccacg gccccagcac cgcgaccacg aagatgtcga
     4141 agtacgcctt cctgtgcgtc agcccgcaga tcgacagcag cgtgatcacc gcgccgttgt
     4201 gcggcaatga atccagcccg ccggtggcca ccgccgtgac gcggtgcagc agcgccggcg
     4261 caatgcccgc ttcctgtccc atcgccagat agctgtcgcc cagcgtctga agcgcgatgc
     4321 tcatcccgcc ggaggcagaa ccggtaatcc ctgcaagtat gttgaccgcg accgccaagg
     4381 agatcagcgg gttgccgggg gcaatgccga gcacgccgtc gcggatcagc gcgaaggccc
     4441 cgagcgatgc aatcaccgcg ccgaagccga ccagcgaagc cgtattgaag atgggcagca
     4501 ccgaggccgc cgcgccgtcg tcgagcgcgg ccttcacgct cggaatgcgg cgcaggttca
     4561 gcgccagcag caccacgatc gacagcgtca gcgcgcagat gatcgaccag acgcccagca
     4621 cctgctgcag gtcggtttca ccatacagcg gcagcgcgag ataagcggtg tcgagcgccg
     4681 gccagatgat ggccgagaac agatagttgg ccgcgatcac cagcacgatg ggcagcaccg
     4741 ccacgaaggc gccgggttct tcggcggccg gtgtgctgcc gccgacttcg gcaagatcat
     4801 agttctcgcc cgcagccagc ggtcgcagtg ccttgtccgg ttgctgcgtc atgccggcct
     4861 ggggggcgtc ggattcgccg tagccctcgc cggcctgcgc ggcgcggcgc gcacgatagg
     4921 ccagccacgc catgcccacg ccgaacatga tggcagccgc aatgatgccg atccccggag
     4981 cggcaaaggg cgtggtgccg aagaagggca tgggaatggc gttctggatc gccggcgtgc
     5041 ccgggagcgc ggtcatcgtg aacgtaaagg cgcccagcgc gatcgtcccc gggatcaggc
     5101 gcttcggcac gttcgcctgg cgaaacagcg cggcggcgat cgggtagacc gcgaaggcga
     5161 ccacgaacag ggacacgccg ccgtaggtca ggatcgcgca ggcgagcacg atcgccagaa
     5221 tcgcgcggcg ctccccgagg cggccgacga tccagcgcgc cagcgcatgg gcgcagcccg
     5281 agacttccat cagtttcccg aacaccgcgc cgagcaggaa cagcgggaag tacagcacga
     5341 cgaaatcgcc gagcgcgcgc atgaacacct gggtgtagct cgcgagcagc ggggagtcgg
     5401 gggcgaacag caccgcgagc agcgcgagga tgggggcgag cagcagaacg gagaagccgc
     5461 ggtacgcgag atagatcagc aggatgaggg cgaggaggat gccgatgatt ccagtcatga
     5521 tgttttcagt gcgcgaaacc ggcgcatcga ggccgccgca tcgaagaatg agcgcgaggc
     5581 gggaagttca ttcccgcaat tggcgctgca tcaagtgaag ggagcgcgat gagtcgtatg
     5641 ggcaactgcg caagtatgcg ggcggaacct gcgcagttgc atgattgagt catggaacgg
     5701 ctagaccggc ggatccccga gcagcatgaa cgccagcgac aggtagatca gcacgccgac
     5761 cgcgtccgca atcgaggtca ccagcggcgc gctggccgtg gctggatcga gccggaagcg
     5821 tgcaaggatg aacggcaggc tcatgccgat caggctgccc atcacgacga tcaggatcat
     5881 cgacgttgcc accacgattg cgatgtcggt gccaccccgc gcgacgccga ttcccgagat
     5941 ggccagcgcc atcgtcagcc cgagcaggcc ggcgaccgtg aactcccgtc ccagcatctt
     6001 cagccagtcg cgcatgtgca cgtcgccggt cgccagcgcg cgcaccatca gcgtggcggc
     6061 ctgggagccg gcgttgccgc cgctgccgac gagcagcgga aggaagaaca ccagcgcgat
     6121 ataggcggcc accgtgtcct cgtaggcggc gatgccgaag ccggaaagca ggttgccaaa
     6181 caccaggatg acca
//
LOCUS       DATLGF010000087         9875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_35862,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000087 DATLGF010000000
VERSION     DATLGF010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 9875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9875
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35862"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VM406_02660"
     CDS             complement(<1..610)
                     /locus_tag="VM406_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74894.1"
                     /translation="MIGSADEDWLRERWLDLVTPYTSDSFGVHATWHALYACYSDPRR
                     SYHNLEHVAALLRLADQERAQICEPEVVEFAIWFHDAIYDTHARDNEQKSAAWAREAL
                     EAISVDPRYVDAVEACILATQNHALGAAAIADLPLFLDLDLAILGAEDADYDDYSRRV
                     REEYAWVPQAQYREGRLAVLRRFAERDRLFLTPSMIARFEARA"
     gene            complement(629..1225)
                     /gene="recR"
                     /locus_tag="VM406_02665"
     CDS             complement(629..1225)
                     /gene="recR"
                     /locus_tag="VM406_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HVL74895.1"
                     /translation="MKSPTSLELLSEALRRLPGVGPKSAQRIAYHLMQHDREGASLLG
                     RALLQAVEKLRHCARCNTFTEQEVCATCQDPERDASLLCVVETPADQLMIEQTMTYKG
                     LYFVLMGRLSPLDGVGPRDLHLEKLVARATDGLVQEVVMATNFTSEGEATAHYISETL
                     KSRGLKVSRLARGVPVGGELEYVDAGTIARAMLDRRAT"
     gene            complement(1360..1686)
                     /locus_tag="VM406_02670"
     CDS             complement(1360..1686)
                     /locus_tag="VM406_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HVL74896.1"
                     /translation="MMKGQLAGLMKQAQAMQDNMKKMQEQLASIEVEGESGAGMVKVV
                     MTCKNDVKRVTIDPSLLGDDKDMLEDLVAAAFNDAVRKAEATSQEKMAGLTAGLPLPP
                     GFKMPF"
     gene            complement(1734..3680)
                     /gene="dnaX"
                     /locus_tag="VM406_02675"
     CDS             complement(1734..3680)
                     /gene="dnaX"
                     /locus_tag="VM406_02675"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HVL74897.1"
                     /translation="MSYQVLARKYRPRSFETLVGQEHVVRALTHALEQKRLHHAYLFT
                     GTRGVGKTTLSRILAKSLNCETGITARPCGTCEACTAIDAGRFVDYIEMDAASNRGVD
                     EMAQLLEQAIYAPSNARFKVYMIDEVHMLTNHAFNAMLKTLEEPPEHVKFILATTDPQ
                     KIPVTVLSRCLQFNLKQMPPGHIIGHLENILGQEGIAFETPALRLLAQGAHGSMRDAL
                     SLTDQAIAYAAGQVTLESVQGMLGALDQSYLIRVLDALAEQDGTALLAVADEMATRSL
                     SYSAALQDLGSLLHRIAMAQSVPAAIGDDLPERAEVLRLAEKFEPEEVQLFYQIAVHG
                     RNELSLAPDEYAGFSMTLLRMLAFRPGHAEAVRAPAEAPAAARPASATRPAAAAQNRE
                     PVAAAAPAAPAAPAAPAAPAMARPAGMSPARAALEAARSGKPLSMPAPAAATAPAPKP
                     VQPVPEPDSGVPPWMDEGPPDEFSARPGSAPAQKKTESAAVELKEPVSATAPVEPPPL
                     ARAPREVAPVPALNWDGDWPTLAAKLPLRGVVHQLAQQSELLECEPHGEGTAFLLRIP
                     VDTLRSAGAVDKLATALSEHFGKPVRVNTEIGAVARTANAAAVAEREARQREAEESMH
                     NDPFVQTLMREFGASFVPGSIRPV"
     gene            complement(3860..3958)
                     /gene="ffs"
                     /locus_tag="VM406_02680"
     ncRNA           complement(3860..3958)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VM406_02680"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(4032..5516)
                     /gene="ubiD"
                     /locus_tag="VM406_02685"
     CDS             complement(4032..5516)
                     /gene="ubiD"
                     /locus_tag="VM406_02685"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041322210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HVL74898.1"
                     /translation="MRYTDLRDFIDQLEKTGELRRVRVPVSPKLEMTEICDRTLRAEG
                     PALLFELPEGHTMAVLGNLFGTTRRVALGMGANDLGELRKIGHVLARLKEPEPPKGFK
                     DVLGLGGLVKSLWDMAPKELRSAPCQEVVWEGGEVDLGRLPIQHCWPGDVAPLITWGL
                     VVTKGPHKKRQNLGIYRQQVLGRNKVIMRWLAHRGGALDFREHARLHPGRPYPVAVAL
                     GADPATILGAVTPVPDSLSEYQFAGLLRGSRTELVKTIGSELRVPASAEIVLEGHIYP
                     DADHPSGFEHALEGPYGDHTGYYNEQDWFPVFTIDRITMRRDPIYHSTYTGKPPDEPA
                     VLGVALNEVFVPLLQKQFAEITDFYLPPEGCSYRMAVVQMRKAYPGHAKRVMFGVWSF
                     LRQFMYTKFIVVVDEDVNVRDWKEVIWAITTRVDPSRDTTLVDNTPIDYLDFASPVSG
                     LGSKMGIDATNKWPGETSREWGRTIEMSPEVKARVDRIWEELGL"
     gene            5959..6876
                     /locus_tag="VM406_02690"
     CDS             5959..6876
                     /locus_tag="VM406_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HVL74899.1"
                     /translation="MSNRWMRSMVEISRREAGRSAAIALVVRDTASGFFTTARHALMM
                     IGFVALCATAALVARPEFGDRALMMLGFAEEAAAEETLSQPPLVAALLPAAPAAAHAV
                     EAATHDAAEELRRQQERVANWIARRYRVASDATHLFVTTAYQTARELKLDPLLILSVM
                     AIESRFNPFAESPAGAQGLMQVMSHIHKDKFEPLGGIEAALNPVANIQVGSRILKEYV
                     TRGGSVEAGLKMYVGAAAFDTDFGYGAKVLAEHARLKDVAMGRKVPIHTRAASAPKPA
                     AAPSAPETAQASAESGQPLAQAEQVASAL"
     gene            complement(6919..8097)
                     /locus_tag="VM406_02695"
     CDS             complement(6919..8097)
                     /locus_tag="VM406_02695"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HVL74900.1"
                     /translation="MQIDHLLAGRLANIAPFHVMELAKMAADLERQGRSIIHMGIGEP
                     DFTAPPPVVEAAMQAMTDGRLQYTAAMGITPLREAISAHYRRTYGLDVAPQRIIVTAG
                     ASAALLLACAALVERDSEVLMPDPSYPCNRHFVAAFEGRARMIASGPAERFQLTEAMV
                     REHWSPATRGVLLASPSNPTGTSIAPDELAQIVAAVREKGGFTIVDEIYQGLSYDAAP
                     TSALSLGEDVVVINSFSKYFNMTGWRLGWLVVPPALVPQIEKLAQNLFICASGVAQHA
                     GLACFSEAALSIYEQRKAEFKRRRDYIVPALEDLGFAVPVRPDGAFYVYADCSRLADD
                     ADRLTIDMLNHAGVVLVPGLDFGPHTARQYIRLSYATSMENLQEAVRRLRVFFDQRSK
                     "
     gene            complement(8104..8574)
                     /gene="nusB"
                     /locus_tag="VM406_02700"
     CDS             complement(8104..8574)
                     /gene="nusB"
                     /locus_tag="VM406_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HVL74901.1"
                     /translation="MSQKSMHANPSKNRTPRHRAREFALQGLYQWLLNQDDAGAIDAH
                     IRQAHGFDKADSEHFDTLLHGTIRQAPQLREGLGPLIDRPLDQLSPVEHAALLIGAYE
                     LSNHIEIPYKVVINEAVELTKSFGGIDGHKYVNGVLDKFAARVRVAETEDAKKR"
     gene            complement(8574..9059)
                     /gene="ribH"
                     /locus_tag="VM406_02705"
     CDS             complement(8574..9059)
                     /gene="ribH"
                     /locus_tag="VM406_02705"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HVL74902.1"
                     /translation="MTVGAYETNLDGESLRIGIVQSRFNEDVCHGLLSACLAELKRLG
                     VADEDVLHVTVPGALEIPLALQKMAETEQFDALIALGAVIRGETYHFELVSNESGAGI
                     TRVSLDFGIPIANAVLTTNTDEQAEVRMAEKGADAARVAVEMANLALALDELAEAGED
                     E"
     gene            complement(9172..>9875)
                     /locus_tag="VM406_02710"
     CDS             complement(9172..>9875)
                     /locus_tag="VM406_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HVL74903.1"
                     /translation="LMRAGHTEAGCDFAQLAGLTPAAVICEILKDDGTMARLPDLIEF
                     ANEHGLKIGTIADLIHYRSRNESIVERIGERSMQTAFGEFKSIAYRDKPSGGAHLALL
                     HGDPRPDLETLVRVHQPVSILDLLETRATTHSWTIASAMEAIRASERGAIVLLNCEES
                     ADEMFAQFNALNSPGDRPQPRGSRMDLRTYGIGAQILKDLGVGKMRLLANPRKMPSMT
                     GFNLEVVGYRACPDN"
BASE COUNT         1655 a         3291 c         3133 g         1796 t
ORIGIN      
        1 gggcccttgc ttcgaagcgc gcgatcatcg acggcgtgag gaacagcctg tcgcgttcgg
       61 cgaagcggcg cagcacggcc agccgccctt cccggtactg cgcctgcgga acccacgcgt
      121 actcctcgcg gaccctgcga ctgtagtcgt catagtcggc gtcctcggca ccgagaatgg
      181 ccagatcgag atcgagaaac aatggcaggt cggcaattgc ggcagccccc agcgcatggt
      241 tttgcgtggc cagaatacag gcctccacgg catccacata tcgcggatcg acgcttatcg
      301 cctccagcgc ctcgcgcgcc caggcggcgc tcttctgttc gttgtcccgc gcatgcgtgt
      361 cgtagatcgc atcgtggaac cagatcgcga actccaccac ttccggctcg cagatctgtg
      421 cgcgctcctg atccgccagc cgcagcagtg cagccacgtg ctccaggtta tggtagctgc
      481 ggcgcggatc ggagtaacag gcgtacaacg catgccaggt cgcgtgcacg ccgaaactgt
      541 cggaggtgta cggcgttacg aggtccagcc agcgctcgcg cagccaatcc tcgtctgcac
      601 taccgatcat catgccctcc tggtcggatc aggtcgcgcg ccggtcgagc atcgcgcgcg
      661 cgatggtccc ggcgtcgacg tactcaagct caccgccgac cggcacgcca cgcgcaagcc
      721 ggcttacctt gagcccgcgc gacttcaggg tttcgctgat gtagtgcgcg gtcgcctcgc
      781 cttcactggt gaaattggtc gccatgacca cctcctgcac gaggccgtcg gtcgcgcgcg
      841 cgaccagctt ctcaagatga agatcgcgcg gacccacccc gtcaagtggc gacagccgcc
      901 ccatcagcac gaagtacagg cccttgtagg tcatcgtctg ctcgatcatc agttgatcgg
      961 ccggcgtctc gacgacgcac agcaagctgg cgtcgcgctc tggatcctgg caggtcgcgc
     1021 agacttcctg ctcggtgaag gtgttgcagc gcgcgcagtg acgcagcttc tccaccgcct
     1081 gcagcagcgc gcggccaagc agcgacgcac cttcacggtc gtgctgcatc aggtggtagg
     1141 cgatgcgctg ggccgacttc gggccgacgc cgggcaaccg gcgcagggcc tccgaaagca
     1201 gttccagact ggtcggcgat ttcacgggat cagggataga ctgggagcca cggcgggcac
     1261 ggcgcaccaa aacgtgcgac cttgcgacga tcggtcttga cgaagcacac gttcatcgcg
     1321 tctggccgtg ctccgcgttg aagtcatcgt caatcggcct cagaacggca tcttgaagcc
     1381 gggcggcagc ggcaggcccg cggtcaggcc ggccatcttt tcctgcgagg tcgcttcggc
     1441 cttgcgcacc gcatcgttga acgcggccgc caccaggtct tcgagcatgt ccttgtcgtc
     1501 gcccagcagg gacgggtcga tcgtcacgcg cttgacatcg ttcttgcagg tcatgacgac
     1561 cttcaccatg ccggcgccgg actcgccctc gacttcgatc gatgcgagct gttcctgcat
     1621 cttcttcatg ttgtcctgca tggcctgcgc ctgcttcatc agtcccgcga gttggccctt
     1681 catcatgatc gctctccttg gtcggtgtgt tcaatgggaa tacggttacc cgctcagacg
     1741 ggccggatgg agccgggcac gaagctcgcg ccgaactccc gcatcagggt ttgcacgaac
     1801 ggatcgttgt gcatgctttc ctcggcttcg cgctggcgcg cctcgcgctc ggccacggca
     1861 gccgcgttgg ccgtgcgcgc caccgcgccg atttcggtat tcacgcgcac cggcttgcca
     1921 aagtgttcgc tcaatgccgt cgcaagcttg tccaccgcgc cggccgaacg cagggtgtcg
     1981 accggaatgc gcaacaggaa cgccgtgccc tcgccgtgcg gctcgcattc gagcagttcg
     2041 ctttgctgtg ccagttgatg gaccacgccc cgtagcggca gcttcgctgc cagcgtgggc
     2101 cagtcgccgt cccagttcag agcgggaaca ggcgcgacct cgcgcggtgc gcgtgccagc
     2161 ggcggcggct cgacgggagc cgttgccgac accggctcct tcagttcaac ggctgcggat
     2221 tcagtttttt tttgagctgg agccgaaccg ggccgcgcgg agaactcatc gggcgggcct
     2281 tcgtccatcc agggcggcac cccggaatcg ggttccggca ccggctggac cggtttcggc
     2341 gccggtgcag tcgctgcggc gggcgcaggc atggaaagcg gcttgccgct gcgtgccgcc
     2401 tcgagcgcgg cgcgtgccgg gctcatgccc gccgggcggg ccattgccgg agccgcgggt
     2461 gccgcaggtg ccgcaggtgc cgcaggtgct gccgccgcaa ccggttcccg attctgtgcc
     2521 gcggccgccg gtcgtgtcgc tgacgcgggg cgagccgcgg caggcgcttc ggccggcgcc
     2581 cgcaccgcct ccgcatgtcc gggtcggaag gccagcatgc gcagcagagt catcgagaaa
     2641 ccggcgtact cgtcgggcgc gagactgagt tcgttgcggc cgtgaacggc gatctgatag
     2701 aacagctgca cttcctccgg ctcgaacttc tccgccaggc gcagcacctc ggcgcgctcg
     2761 ggcaggtcgt cgccgatcgc ggccggcacc gactgcgcca ttgcgatccg gtgcaacagg
     2821 ctgcccaggt cctgcagtgc ggcgctgtag gacaggctgc gcgtcgccat ctcgtctgcc
     2881 accgcaagca aggcggttcc gtcctgctcg gccagcgcat caagcacacg gatcaggtag
     2941 gactggtcga gcgcaccgag catgccctgc accgattcca gcgtcacctg accggccgca
     3001 taggcgatcg cctgatcggt cagcgacagc gcatcgcgca tagacccatg cgcaccctgc
     3061 gcgagcaggc gcagcgccgg cgtctcgaac gcgatgcctt cctggccgag gatgttctcg
     3121 agatggccga tgatatgacc cggcggcatc tgcttcaggt tgaactgcag gcagcgcgac
     3181 agcacggtga cgggaatttt ctgcggatct gtggtcgcga ggatgaactt cacatgctcc
     3241 ggcggctctt ccagcgtctt cagcatcgca ttgaacgcgt ggttggtcag catgtgcact
     3301 tcgtcgatca tgtagacctt gaagcgcgcg ttcgaaggtg cgtagatcgc ctgttccagc
     3361 aactgcgcca tttcatcgac gccgcggttc gaggctgcgt ccatctcgat gtagtcgacg
     3421 aagcgtccgg catcaattgc cgtgcaggct tcgcatgtcc cgcagggccg cgcggtgatg
     3481 ccggtttcgc agttgagcga cttggcgaga atgcgcgaga gcgttgtctt gcccactccg
     3541 cgcgtgccgg taaacagata cgcatggtgg agccgttttt gctccagtgc atgggtcaac
     3601 gcacgcacga catgctcctg cccgaccaac gtctcgaagc tgcgggggcg atacttgcgg
     3661 gcgagaacct gataggacat gggaggcgtg cgtgaatgcg tttgctgcaa cagccggaat
     3721 tttacccgac ctgtgcgcgg tcccaagcgc ggttcgattg cgggcacgca tgaaagacgg
     3781 catcaaccca tggcgacact gtcgagcatg cgggataagg cggacgaaac gtcgcggcag
     3841 gaaggaggaa tgagaataag ggggcgagcc ttgtctgcgg cacttgcggg aaatggctgt
     3901 ggctgcttcg ttcccgacct gaccaggttg accacgccgc aatgcgcagg ggcccgccga
     3961 cctgcattct atcagcatcg tggggtgctg tcctctgatg aaacatcaag gcaacgctgc
     4021 tttcctgccc actaaagccc cagttcctcc cagatccggt ccaccctggc cttcacctcc
     4081 gggctcatct ctattgtccg cccccactcg cggctggtct cgcccggcca cttgttggtc
     4141 gcgtcgatgc ccatcttgct gcccaggccg ctgaccggtg atgcgaaatc gaggtagtcg
     4201 atcggcgtgt tgtcgaccag cgtggtgtcg cgcgacggat ccacgcgcgt ggtgatcgcc
     4261 cagatcacct ccttccagtc gcgcacgttc acatcctcgt cgacgacgac aatgaacttc
     4321 gtatacatga actgacgcag gaagctccag accccgaaca tcacgcgctt cgcatggcca
     4381 ggataggcct tgcgcatctg gaccaccgcc atccggtagc tgcagccctc gggcggcagg
     4441 tagaaatcgg taatctcggc gaactgcttc tgcagcagcg gcacgaacac ttcattgagc
     4501 gccacgccga gcacggccgg ctcgtccggc ggcttgccgg tgtaggtcga gtgatagatt
     4561 ggatcgcgcc gcatcgtgat gcggtcgatc gtgaagaccg gaaaccagtc ctgctcgttg
     4621 taatagccgg tgtgatcgcc gtacggacct tcgagcgcat gttcgaagcc gctgggatga
     4681 tcggcatccg gatagatatg gccttcgaga acgatctccg ccgaggccgg cacccgcagt
     4741 tcactgccga tcgtcttgac cagctccgta cggctgccgc gcagcaggcc ggcgaactgg
     4801 tactcggaca gactgtccgg caccggcgtc accgcgccca gtatcgttgc cggatccgcg
     4861 ccgagcgcca ccgccaccgg atacggccgc cccggatgaa ggcgcgcatg ctcgcggaaa
     4921 tcgagcgccc caccgcgatg cgcgagccag cgcatgatta ccttgttgcg ccccagcacc
     4981 tgctggcggt agatgccgag attctgccgc ttcttgtgcg gccccttggt gaccacgagg
     5041 ccccaggtga tcaacggggc gacgtcgccc ggccagcagt gctggatcgg cagcctgcca
     5101 aggtcgacct cgccgccctc ccacacgact tcctggcaag gcgccgagcg cagctccttc
     5161 ggggccatgt cccacagcga tttcaccagc ccgcccagcc cgagcacatc cttgaagccc
     5221 ttcggcggtt ccggctcctt caggcgcgcc agcacgtggc cgatcttgcg cagctcgccc
     5281 aagtcgttcg cgcccattcc aagagcgacg cgccgcgtgg tgccgaacag attccccagc
     5341 acggccatcg tatgcccctc tggtagctcg aacagcaacg ccggcccctc cgcgcgcagc
     5401 gtgcggtcgc agatctcggt catttcaagc ttcggggaca ccggcacgcg cacgcgccgc
     5461 agttctccag tcttctccag ctggtcaatg aagtcgcgca ggtcggtata tctcatgatt
     5521 tttcacaatg ctggaacctg ctttccattc tctaatacga gcgccaagcc cttgatttcc
     5581 aagacctccc gatgcgtcgg cgcaaggttt ctatatttag aagtaataag aaatagattc
     5641 tatagtattg acgcgttata aacagctccc tacaattcgc gcaactttga acgaggggtg
     5701 gatttccggc cgaaccagcc ggctcgagac gtccactcac tgccaacggg ttccgggtcc
     5761 cacattgtaa ggagtgtttc acaaggcgtc cgccttgcac ccgcaaaagt tgccatatcg
     5821 agcggccgcc cggtcgcctg gcaactccgc cggccggcat ctggcgctgc gcgccgatcc
     5881 cagccgatgt cactgattcc gaggcgtggc ccgcctgcaa gcaggaaggg tcgtgctccg
     5941 gcgattcagg aggtatatat gtcaaaccga tggatgcgct ccatggtcga gatcagccgg
     6001 cgcgaggccg gtcggtccgc cgccattgcg ctcgtcgtgc gcgataccgc gagcggattc
     6061 ttcacgaccg ctcgccacgc actgatgatg atcggcttcg ttgcactgtg cgcaacggca
     6121 gcgctcgtcg cgcggcccga gttcggcgac cgcgcgctga tgatgctcgg cttcgccgag
     6181 gaggcggccg ccgaggagac tctgtctcag ccccctctcg ttgccgcgct gttgcccgcc
     6241 gcacccgctg ccgcgcacgc ggtcgaagcg gccacccacg acgctgccga ggaattgcgt
     6301 cggcagcagg aacgggtcgc caactggatt gcccgccgct accgggtcgc cagcgacgcc
     6361 acccacctgt tcgtcaccac cgcctaccag acggccaggg aactcaagct tgacccgctg
     6421 ctcattctgt ctgtgatggc gatcgaatcg cgcttcaatc cgttcgcgga gagtccggcc
     6481 ggtgcgcagg gcctgatgca ggtgatgtcg cacatccaca aggacaagtt cgagccgctc
     6541 ggcggcatag aggcggcgct gaacccggtt gccaacatcc aggtcggctc gcgcatcctg
     6601 aaggaatacg tgacccgcgg cggatcggtc gaggctggcc tgaagatgta cgtgggtgcc
     6661 gccgcgttcg acacggactt cggctatggg gcgaaggtgc tggccgaaca tgcgcgcctg
     6721 aaggatgtgg cgatgggccg caaggtcccg atccatacgc gcgccgcctc ggccccgaag
     6781 cccgctgctg cgccgagcgc gcctgagacg gcgcaggcga gcgccgaatc gggccagccg
     6841 ctcgcccagg cagagcaggt ggcgtcggcg ctttgaacac cgagtcagtc acaaaaaaag
     6901 gagccaatgg ctcctttttc atttcgaccg ctgatcgaag aacaccctga gccgccgcac
     6961 tgcctcctgc aggttctcca ttgaagtggc atacgacagc cggatgtact gccgcgccgt
     7021 atgcggcccg aaatcgagac ccgggaccag caccaccccc gcatggttga gcatgtcgat
     7081 ggtcaggcgg tccgcgtcat ccgccagcct ggaacagtcg gcgtacacgt agaacgcgcc
     7141 gtcgggcctg accgggaccg cgaatccgag atcctcgagt gccggaacga tgtagtcgcg
     7201 ccgccgcttg aattccgcct tgcgctgttc gtagatggac agcgcggctt cgctgaagca
     7261 ggccagtccg gcatgttgcg cgacgccaga cgcgcagatg aacaggtttt gcgcgagctt
     7321 ttcgatctgc gggaccagcg ccggcggcac caccagccag ccgaggcgcc agcctgtcat
     7381 gttgaagtac tttgagaagc tgttgatgac gaccacatcc tcgccgagcg acagcgccga
     7441 ggtcggcgcc gcgtcgtagc tcaaaccctg gtagatctcg tcgacgatgg tgaacccgcc
     7501 cttctcgcgc acagccgcga cgatctgcgc cagctcgtcc ggcgcgatcg aggttccggt
     7561 cgggtttgac ggcgatgcca gcagcacgcc gcgtgtcgcg ggcgaccagt gctcgcgcac
     7621 catcgcctcg gtcagctgga agcgctcggc cgggccgctg gcaatcatac gcgcccgccc
     7681 ctcgaacgcg gcgacgaaat gtcggttgca cggataggat ggatcgggca tcagcacttc
     7741 gctgtcccgc tccaccagcg cggcgcaggc cagcagcagc gccgccgagg cgccggccgt
     7801 gacgatgatg cgctgcggcg ccacgtccag cccgtaggtg cggcgatagt gggccgaaat
     7861 cgcctcccgc agcggcgtga tgcccatcgc ggcggtgtac tggaggcgac cgtcggtcat
     7921 cgcctgcata gcggcctcga cgaccggcgg gggcgcggtg aagtccggct cgccgatccc
     7981 catgtgaatg atggaacgtc cctggcgctc caggtccgca gccatcttcg ccagctccat
     8041 cacgtggaaa ggcgcgatat tggccaggcg tcccgccagc aagtgatcga tttgcatgcc
     8101 gcttcagcgc ttctttgcgt cttcggtctc cgccacgcgc accctggcgg cgaacttgtc
     8161 gagcacgccg ttcacatact tgtgcccgtc gatgccgccg aaggacttgg tcagctcgac
     8221 cgcctcgttg atcaccacct tgtacgggat ttcgatgtga ttgctcagtt cgtaggcgcc
     8281 gatcagcagc gccgcgtgct cgaccgggga aagctggtcg agcggccggt cgatcaaggg
     8341 accgaggcct tcgcgcagct gcggcgcctg ccggatcgtg ccgtgcagca aggtgtcgaa
     8401 atgctcggaa tccgccttgt cgaaaccgtg ggcttggcgg atgtgggcat cgatggctcc
     8461 ggcgtcatcc tggttcagca accactggta tagcccctgc aatgcgaact cgcgcgcgcg
     8521 gtggcgcggc gtgcggttct tgctcgggtt ggcatgcatg ctcttctgcg acatcactcg
     8581 tcctcgccgg cctcggccag ctcatcaagc gccagagcca gattcgccat ctcgacggcc
     8641 acgcgtgcgg cgtcggcgcc cttctcggcc atgcgtacct cggcctgctc gtctgtattc
     8701 gtggtcagca ccgcattggc gatcgggatg ccgaagtcga gcgacacgcg cgtgatgccg
     8761 gcgcccgact cgttcgagac cagctcgaaa tgataggttt ccccgcgtat gaccgcgccc
     8821 aacgcgatca gtgcatcgaa ctgctcggtt tccgccatct tctgcagcgc cagcgggatt
     8881 tccagagcgc cgggcacggt aacgtgcagc acgtcctcgt ctgccacgcc aagccgcttg
     8941 agctcggcca ggcaagccga cagcagtccg tggcagacgt cttcgttgaa gcgggattgc
     9001 acgatgccga tgcgcaggct ttcgccatcg agattcgttt cgtaggcacc taccgtcatg
     9061 atggtttcct ttggcacggg gccgttatcg cggatggccc ggcgcacagg gcgctgtttc
     9121 catccgcggt gttgaaagaa agacgccggc ccagtgccgg cgatcagaga ttcagttgtc
     9181 cggacacgcc cggtagccga ccacttcgag gttgaagccc gtcatcgacg gcatcttgcg
     9241 cggattggcc agcagacgca tcttgccgac gccgagatcc ttcaggatct gggcgccgat
     9301 gccgtaggtg cgcaggtcca tgcgcgagcc acgcggctgc ggccggtcgc cgggcgagtt
     9361 cagcgcattg aactgcgcaa acatctcgtc ggccgattct tcgcaattga gcaggacgat
     9421 ggcgccgcgc tcgcttgcgc ggatcgcttc catcgccgac gcgatggtcc acgaatgggt
     9481 cgtggcgcgg gtttcaagca gatcgaggat ggataccggc tgatgcacgc gcaccagcgt
     9541 ctcgagatca ggccgcggat cgccgtgcag cagcgccagg tgggcgccgc cactgggctt
     9601 gtcgcggtac gcaatgctct tgaactcccc gaacgccgtc tgcatgctgc gctcgccgat
     9661 gcgttcgacg atgctttcgt tgcggctgcg gtagtggatc aggtcggcga tcgtgccgat
     9721 cttcagaccg tgctcgttgg cgaactcgat caggtccggc aggcgcgcca tcgtgccgtc
     9781 atccttcagg atctcgcaga tcacggcggc gggcgtcagg cccgccagct gcgcgaagtc
     9841 gcaaccggct tcggtatggc cggcgcgcat cagca
//
LOCUS       DATLGF010000088         8826 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_113079, whole genome shotgun sequence.
ACCESSION   DATLGF010000088 DATLGF010000000
VERSION     DATLGF010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 8826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8826
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113079"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1671
                     /gene="rpoB"
                     /locus_tag="VM406_02715"
     CDS             <1..1671
                     /gene="rpoB"
                     /locus_tag="VM406_02715"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465494.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HVL74904.1"
                     /translation="WNGYNFEDSILISEKVVSDDRYTSIHIEELSVLARDTKLGPEDI
                     TRDISNLSESQLSRLDESGIVYIGAEVQAGDTLVGKVTPKGETQLTPEEKLLRAIFGE
                     KASDVKDTSLRVDQGSQGTVIDVQVFTREGIQRDKRAQQIIDDELKRFRLDLNDQLRI
                     VEADAYDRIEKLLTGKVANGGPNKLAKGTKIDKAYLQSIDKYHWFDIRPADDDVAAQL
                     ESIKNSIEQQRHSFDLAFEEKRKKLTQGDELPAGVLKMVKVYLAVKRRLQPGDKMAGR
                     HGNKGVVSRIVPVEDMPYMADGTPADIVLNPLGVPSRMNVGQVLEVHLGWASKGLGLR
                     IGEMLRAQQKAAELRAFLGKIYNESGRSEDLDSLSDEEILTLANNLKHGVPFATPVFD
                     GAHEGEIRRMLDLAYPDEIATQLGMTPSKNQVTMYDGRTGEAFERPVTVGYMHVLKLH
                     HLVDDKMHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASYVLQEMLTVK
                     SDDVTGRTKVYENLVKGDHVIDAGMPESFNVLVKEIRSLGIDIDLERE"
     gene            1763..6004
                     /gene="rpoC"
                     /locus_tag="VM406_02720"
     CDS             1763..6004
                     /gene="rpoC"
                     /locus_tag="VM406_02720"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HVL74905.1"
                     /translation="MKALLDLFKQVQQSEQFDAIKIGLASPEKIRSWSYGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPIKDYECLCGKYKRLKHRGVICEKCGVEVTLAKVRRERM
                     GHIELASPTAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAYVVTDPGMTPLKKCQIM
                     SEDDYAAKYEEYGDDFTAFMGAEGIRELLRTIDIDREVDQLRQELKDSKSEAKIKKYA
                     KRLKVLEAFQRSGIKPEWMIMEVLPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLMELRAPEIITRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLAEMIKGK
                     GGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKLMALELFKPFIFNKLELMGLAT
                     TIKAAKKLVEMQEPVVWDILEEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAFNADFDGDQMAVHVPLSIEAQMEARTLMLASNNVLFPSNGEPSIVPSQDIVL
                     GLYYATREKINGRGEGMMFADVGEVIRAYDNKEIELTSRITVRITEYPKDPATGEFVK
                     TVNRYETTVGRAILSEILPKGLPFPVLNKALKKKEISKLINTSFRKCGLRATVVFADQ
                     LMQSGFRLATRAGISICVDDMLVPPQKATIIGQAESEVKQIEQQYASGLVTAGERYNK
                     VVDIWGKAGDEVGKAMMEQLKVEDVTKRDGSKGVQESFNAIYMMADSGARGSAAQIRQ
                     LAGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLT
                     RRLVDVTQDLVVTEDDCGTANGASMKALVEGGEVIEALRDRILGRVAANDVINPENQE
                     TLYEAGTLLDEDAVETIEALGIDEVKVRTPLTCDTRYGLCAKCYGRDLGRGMLVNAGE
                     AVGVVAAQSIGEPGTQLTMRTFHIGGAASRAAVASSVEAKSNGTVRFTATMRYVTNGK
                     GEQIVISRSGEVLITDDIGRERERHKVPYGATLLVKDGLAIKAGTALANWDPLTRPII
                     TEYAGTVKFENVEEGVTVAKQIDEVTGLSTLVVIDAKRRGSSTKVMRPQVKLLNEKGE
                     EVKIAGTEHAVTIGFQVGALITVKDGQQVSVGEVLARIPTESQKTRDITGGLPRVAEL
                     FEARSPKDAGMLAEVTGTVAFGKETKGKQRLEITDMDGNKHEFLITKDKQVLVHDGQV
                     VNKGEMIVDGPADPQDILRLLGIEALARYIVDEVQDVYRLQGVKINDKHIEVIVRQML
                     RRVQVVDAGDTNYIPGEQVERSELLDENDRVIAEGKRPATYDNVLLGITKASLSTDSF
                     ISAASFQETTRVLTEAAIMGKKDGLRGLKENVIVGRLIPAGTGLAYHRARKEKETWEA
                     EERSALLLAEESARSAEAEAAHAEGGEPQQDEGQA"
     gene            6333..6716
                     /gene="rpsL"
                     /locus_tag="VM406_02725"
     CDS             6333..6716
                     /gene="rpsL"
                     /locus_tag="VM406_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HVL74906.1"
                     /translation="MPTINQLIRKPRVSAVVKSKSPALENSPQKRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGFEVISYIGGEGHNLQEHSVVLLRGGRVKDLPGVRYHMVRGAL
                     DTQGVKDRKQARSKYGAKRAKAAKK"
     gene            6879..7349
                     /gene="rpsG"
                     /locus_tag="VM406_02730"
     CDS             6879..7349
                     /gene="rpsG"
                     /locus_tag="VM406_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HVL74907.1"
                     /translation="MPRRREVPKREILPDPKFGNVEVAKFVNVLMLSGKKSVAENIIY
                     GAVDHIQQKSGKDPLEVFTAAINNCKPMVEVKSRRVGGANYQVPVEVRPVRRLALSMR
                     WLREAANKRSEKSMPQRLGGELMEAAEGRGGAMKKRDEVHRMAEANKAFSHFRF"
     gene            7515..>8826
                     /gene="fusA"
                     /locus_tag="VM406_02735"
     CDS             7515..>8826
                     /gene="fusA"
                     /locus_tag="VM406_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006482929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HVL74908.1"
                     /translation="MARKTPIERYRNIGISAHIDAGKTTTTERILFFTGVNHKLGEVH
                     DGAATMDWMEQEQERGITITSAATTCFWKGMAGNFPEHRINIIDTPGHVDFTIEVERS
                     MRVLDGACMVYCAVGGVQPQSETVWRQANKYRVPRLAFVNKMDRTGANFFKVYDQMRA
                     RLKANPVPIQVPIGAEDTFEGVVDLIKMKAIYWDEASQGTKFEYRDIPAELQATSDEW
                     REKMVEAAAEANEELMNKYLEEGELSEEEIKKAIRLRTIAGEIVPMMCGTAFKNKGVQ
                     AMLDAVIEYLPAPTDIPPVQGTDENEQPTSRKADDNEKFSALAFKIMTDPFVGQLIFF
                     RVYSGVVNSGDTVYNSVKGKKERLGRILQMHANQREEIKEVRAGDIAAAVGLKEATTG
                     DTLCDPDSIVTLERMVFPEPVISQAVGLGLRLDRLRDHRLREHHA"
BASE COUNT         1879 a         2655 c         2813 g         1479 t
ORIGIN      
        1 tggaacggct acaacttcga ggactcgatc ctgatctcgg agaaggtggt ttccgacgac
       61 cgctacacgt cgatccacat cgaggagctg tcggtgctcg cgcgcgacac caagctcggt
      121 cccgaggaca tcacgcgcga catctcgaac ctgtcggaga gccagctgtc gcgcctggac
      181 gagtcgggaa tcgtatacat cggcgccgag gtgcaggccg gcgacacgct ggtgggcaag
      241 gtcacgccga agggcgagac gcagctgacg ccggaagaga agctgctgcg cgcgatcttc
      301 ggcgagaagg cgtccgacgt gaaggacacc tcgctgcgcg tcgaccaggg ctcgcaaggc
      361 accgtcatcg acgtgcaggt cttcacccgc gaaggcatcc agcgcgacaa gcgggcccag
      421 cagatcatcg acgatgagct caagcgcttc cgcctggacc tgaacgacca gctgcgcatc
      481 gtcgaagccg acgcgtacga ccggatcgag aagctcctca cgggcaaggt ggccaacggc
      541 ggcccgaaca agctcgccaa gggcaccaag atcgacaagg cctacctgca gtcgatcgac
      601 aagtaccact ggttcgacat ccgccctgcg gacgacgacg tggccgcgca gctcgagtcg
      661 atcaagaact cgatcgagca gcagcgccac agcttcgacc tggcgttcga ggaaaagcgc
      721 aagaagctca cgcagggcga cgaactgccg gcgggcgtgc tcaagatggt caaggtctac
      781 ctggccgtca agcgccgcct gcagcccggt gacaagatgg ccggtcgtca cggcaacaag
      841 ggtgtggtgt cgcgcatcgt gccggtcgaa gacatgccgt acatggctga cggcacgccg
      901 gccgacatcg tgctgaaccc gctcggcgtg ccgtcgcgga tgaacgttgg tcaggtgctc
      961 gaggttcacc tcggctgggc atctaagggt cttggcctgc gcatcggcga aatgctgcgc
     1021 gcacagcaga aggcggccga acttcgcgcc ttcctcggca agatctacaa cgaatccggc
     1081 cgcagcgaag acctcgacag cctgtcggat gaggaaatcc tcacgctcgc gaacaacctg
     1141 aagcatggcg tgccgttcgc aaccccggtg ttcgacggcg cgcacgaagg cgagatccgc
     1201 cgcatgctgg atctggccta tccggacgag atcgcgaccc agctcggcat gacgccgtcg
     1261 aagaaccagg tcacgatgta cgacggccgc acaggcgaag ccttcgagcg cccggtcacg
     1321 gtcggctaca tgcacgtgct caagctgcac cacctggtcg acgacaagat gcacgcacgc
     1381 tccaccggcc cgtactcgct ggtgacccag cagccgctgg gcggcaaggc gcagttcggc
     1441 ggccagcgct tcggtgaaat ggaagtgtgg gcgctggaag cctatggcgc gtcgtatgtg
     1501 ctgcaggaga tgctcaccgt gaagtcggac gacgtcaccg gccgtaccaa ggtctacgag
     1561 aacctggtca agggcgatca cgtaatcgac gccggcatgc cggagtcctt caacgtgctg
     1621 gtgaaggaaa tccgctcgct gggtatcgat atcgacctgg aacgcgagta atcgggcgca
     1681 ggcgccggcc gcatgcagat gcgcccggcg cggaactacc gaagcaacag atattttgat
     1741 tcacgccact ctggagtgat agatgaaagc actgctcgat ctattcaagc aagttcagca
     1801 aagcgaacaa ttcgacgcga tcaagatcgg cctcgcgtcg cccgagaaga tccgttcctg
     1861 gtcctacggt gaagtcaaga agccggagac catcaactac cgtacgttca agcccgagcg
     1921 cgacggcctg ttctgcgcca agatcttcgg cccgatcaag gactacgaat gcctgtgcgg
     1981 aaagtacaag cggctgaagc accgcggcgt gatctgcgag aagtgcggcg tggaggtcac
     2041 gctggccaag gtgcgccgcg agcgcatggg ccacatcgag ctggcctcgc cgaccgcgca
     2101 tatctggttc ctgaagtcgc tgccgtcgcg tctgggcatg gtgctcgaca tgacgctgcg
     2161 cgacatcgag cgcgtgctgt atttcgaagc ctacgtcgtg accgatccgg gcatgacccc
     2221 gctgaagaag tgccagatca tgtcggaaga cgactacgcg gccaagtacg aagagtacgg
     2281 cgatgacttc accgccttca tgggcgccga aggcattcgc gagctcttgc gaacgatcga
     2341 catcgaccgc gaggtcgacc agctgcgcca ggagctgaag gattcgaagt ccgaagccaa
     2401 gatcaagaag tacgccaagc gcctgaaggt gctcgaggcc ttccagcgtt ccggcatcaa
     2461 gcccgagtgg atgatcatgg aagtgctgcc ggtcctgccg ccggaactgc gtccgctggt
     2521 gccgctggat ggcggccgct ttgcgacctc cgacctgaac gatctgtatc gccgcgtcat
     2581 caaccgcaac aatcgtttga agcggctgat ggagctgcgt gcgccggaaa tcatcacgcg
     2641 caatgaaaag cgcatgctgc aggaagccgt cgactcgctg ctcgacaatg gccgtcgcgg
     2701 caaggccatg actggcgcca acaagcgtcc gctgaagtcg ctcgctgaaa tgatcaaggg
     2761 caagggcggc cgcttccgtc agaacctgct cggcaagcgc gtcgactatt ccggccgttc
     2821 ggtcatcgtc gtgggcccgc aactgaagct gcaccagtgc ggcctgccga agctgatggc
     2881 gctcgaactg ttcaagccct tcatcttcaa caagctcgag ctgatgggcc tggcaacgac
     2941 gatcaaggcc gccaagaagc tggtcgagat gcaggaaccg gtggtctggg acatcctgga
     3001 agaagtgatt cgcgaacatc cggtcctgct caaccgcgcg ccgacgctgc accgtctggg
     3061 catccaggcg tttgagccgg tgctgatcga aggcaaggcg atccagctgc atccgctggt
     3121 ctgcgcggca ttcaacgccg acttcgacgg cgaccagatg gctgtccacg tcccgctgtc
     3181 gatcgaggcg cagatggaag cccgcacact gatgctggcc tccaacaacg tgctgttccc
     3241 gtccaacggc gagccgtcga tcgtcccgtc tcaggacatc gtgctcggtc tgtactacgc
     3301 aacccgcgag aagatcaacg gccgcggcga ggggatgatg ttcgccgatg tgggcgaagt
     3361 gatccgcgcc tacgacaaca aggaaatcga gctgacctcg cgcatcaccg tgcgcatcac
     3421 cgagtacccg aaggacccgg ccaccggtga gttcgtgaag acggtgaacc gctatgagac
     3481 gactgtcggc cgtgcgatcc tgtccgagat cctgccgaag ggcctgccgt tcccggtgct
     3541 gaacaaggcg ctgaagaaga aggaaatctc gaagctgatc aatacctcgt tccgcaagtg
     3601 cggcctgcgt gcgaccgtcg tgttcgccga tcagctgatg cagtcgggct tccgcctggc
     3661 gacccgcgcc ggcatctcga tctgcgtgga tgacatgctg gtgccgccgc agaaggcgac
     3721 catcatcggc caagccgaat cggaagtgaa gcagatcgag cagcagtacg cgtccggtct
     3781 tgtgacggct ggcgagcgct acaacaaggt ggtcgacatc tggggcaagg ccggcgacga
     3841 agtcggcaag gccatgatgg agcagttgaa ggtcgaggac gtgaccaagc gcgacggcag
     3901 caagggcgtg caggaatcgt tcaacgcgat ctacatgatg gccgattccg gtgcacgggg
     3961 ttccgccgcg cagatccgcc agcttgccgg catgcgcggc ctgatggcca agccggacgg
     4021 ttcgatcatc gaaacgccga ttaccgcgaa cttccgcgag ggcttgaacg ttctgcagta
     4081 cttcatctcg acccacggcg cccgcaaggg tctggccgat acagcgctga agactgcaaa
     4141 ctccggttac ctgacccgtc gtctcgtcga cgtgacccag gatctggtgg ttaccgaaga
     4201 cgattgcggc accgccaacg gcgcgtcgat gaaggcgctg gtcgaaggtg gtgaggtgat
     4261 cgaagcgctg cgcgaccgta tcctcggccg cgtggcagcg aacgacgtca tcaatccgga
     4321 aaaccaggaa acgctgtacg aggccggcac gctgctggac gaagacgcgg tcgagacgat
     4381 cgaggcgctg ggcatcgacg aagtgaaggt acgcacaccg ctcacttgcg acacacgcta
     4441 cggtctgtgc gcgaagtgct acggccgcga ccttggccgc ggcatgctgg tcaatgccgg
     4501 cgaagccgtc ggtgtggtgg cagcgcagtc gatcggtgaa ccgggtaccc agctgacgat
     4561 gcgtaccttc cacatcggtg gtgcggcatc gcgtgcagcg gtggcctcct cggtggaagc
     4621 caagtcgaac ggcacagtgc gcttcaccgc gaccatgcgt tacgtgacca acggcaaggg
     4681 cgaacagatc gtcatttccc gttccggcga agtgctgatc acggacgaca tcggccgcga
     4741 gcgcgagcgt cacaaggtgc cctacggtgc aacgctcctg gtcaaggatg gcctggcgat
     4801 caaggctggt accgcactgg cgaactggga cccgctgacc cgtccgatca tcaccgagta
     4861 cgcaggtacc gtgaagttcg agaacgtgga agaaggcgtg accgtcgcca agcagatcga
     4921 cgaagtaacc ggtctgtcga ccctggtcgt cattgacgcc aagcgccgtg gttcctccac
     4981 caaggtcatg cgtccgcagg tgaagctgct caacgagaag ggcgaggaag taaagatcgc
     5041 cggcaccgaa catgcggtga cgattggctt ccaggtcggc gcgctgatca ccgtgaagga
     5101 cggccagcag gtatcggtcg gtgaagtgct cgcacgtatc ccgaccgaat cgcagaagac
     5161 ccgtgacatt accggcggtc tgccgcgtgt ggctgaactg ttcgaggccc gttcgccgaa
     5221 ggatgccggc atgctggcgg aagtcaccgg tacggtggca ttcggcaagg agaccaaggg
     5281 caagcagcgt ctggaaatca ctgacatgga cggcaacaag cacgagttcc tgatcaccaa
     5341 ggacaagcag gtgctggtgc atgacggcca ggtggtgaac aagggcgaaa tgatcgtgga
     5401 cggtccggca gatccgcagg acatcctgcg tctcttgggc atcgaggcgt tggcgcgcta
     5461 catcgtcgac gaggtgcagg acgtctaccg tctgcagggc gtgaagatca acgacaagca
     5521 catcgaggtg atcgtgcgcc agatgctgcg gcgcgtgcag gtcgtggacg ccggggatac
     5581 caactacatc ccgggcgaac aggtcgagcg ctccgagctg ctggacgaaa acgaccgcgt
     5641 catcgccgaa ggaaagcgtc cggccaccta cgacaacgtg ctgctgggta tcaccaaggc
     5701 ctcgctgtcg accgactcct tcatctcggc cgcatcgttc caggaaacca cgcgtgtcct
     5761 gaccgaagcc gcgatcatgg gcaagaagga cggcctgcgt ggtctgaagg aaaacgtcat
     5821 cgtgggtcgc ctgatcccgg cgggtaccgg cctggcttat caccgcgccc gcaaggagaa
     5881 ggagacttgg gaagccgaag agcgcagcgc gctgctgctg gcggaggaat ccgcccgttc
     5941 ggccgaagcc gaggcggccc atgcagaagg gggcgagccg cagcaggacg agggccaagc
     6001 ctgattcagt cagggcgctt gactgcgcaa aaacggcacc gcacagcggt gccgtttttt
     6061 attcctgaga gcggcggatg caaagctgca ttgacagtcg cagccgttct ccgttaaact
     6121 cgcgagttct cgatttcagg ctttcccggg cctgggcgtt ctttggtctg caggtcttgt
     6181 cgttttcccg ccttccgcaa cgctcgcccc cggcttgaat cgcgcccgaa tcgaacccga
     6241 aacgggcacc tgaaagggaa gcttcccctc ccggcgccgg atttttcctg cgtcgggttt
     6301 gttcatgtag ttactgtttt ttttaaagac cgatgccaac aatcaatcaa ctgattcgca
     6361 agccgcgggt ttccgcggtt gtgaagagca agtcgccggc gctggaaaac agcccgcaga
     6421 aacgcggcgt ctgcacacgc gtctacacca cgaccccgaa gaagccgaac tcggcgctgc
     6481 gtaaggtcgc caaggtgcgc ctgaccaacg gtttcgaagt catttcgtat atcggcggcg
     6541 aaggccacaa tctgcaggaa cacagcgtcg tgctgctgcg cggcggccgt gtgaaggatt
     6601 tgccgggtgt gcgttaccac atggtgcgcg gcgcactgga tacccagggc gtcaaggacc
     6661 gcaagcaggc ccgctcgaag tatggtgcca agcgcgccaa ggctgccaag aagtaactga
     6721 attttccgtc gtgtgccact ctgttggcgc ggaggcggat tgagtcggcc cggagtgcga
     6781 atgattgggg tcgagtaagt gggtgatcag gatggtcatc cgggagtgcg ggcgaaattc
     6841 tccacgccgc gctcaactga agagagaaag gaatcgaaat gccacgtcgt cgtgaagtcc
     6901 ccaagcggga aatcctcccg gatccgaagt tcggcaatgt cgaagttgcc aagttcgtca
     6961 acgtcctgat gctgtccggt aaaaaatcgg tcgccgagaa catcatctat ggtgcggttg
     7021 accacatcca gcaaaagagc ggcaaggatc cgctggaagt cttcaccgcc gccatcaaca
     7081 actgcaagcc gatggtcgaa gtgaagtcgc gccgcgtcgg cggtgcgaac taccaggtgc
     7141 cggttgaggt gcgcccggtg cgccgcctcg cgctgtccat gcgctggctg cgtgaagctg
     7201 cgaacaagcg cagcgaaaag tcgatgcccc agcgtctggg cggtgagttg atggaagctg
     7261 ctgaaggccg tggcggcgcc atgaagaagc gtgacgaagt tcaccgcatg gctgaggcga
     7321 acaaggcgtt ctcgcacttc cgcttctaaa gagaatcaaa acgatcgttg ccttcgcatc
     7381 gctgaaggcg ttgcagcaac agggtcaaaa ccgggcgatt accgcaaagc ggcgctcggt
     7441 tttggcccat ctggattaac ccggatttaa cccgatcccc gcacgcatgg cgcggggcca
     7501 gcttaggatt gaatatggct cgcaagaccc ccatcgagcg ctaccgcaac atcggtattt
     7561 ccgctcatat tgacgccggt aaaaccacca cgaccgagcg tatcctgttc ttcaccggtg
     7621 tgaaccacaa gctgggtgaa gtgcacgatg gcgctgcaac catggactgg atggagcagg
     7681 agcaagagcg cggcatcacg atcacgtccg ccgccaccac ctgcttctgg aagggcatgg
     7741 ccggcaattt ccccgagcac cgcatcaaca tcatcgacac cccgggtcac gtggacttca
     7801 ccatcgaggt ggagcgttcg atgcgcgttc tcgacggcgc ctgcatggtc tactgcgccg
     7861 tgggtggcgt gcagccgcag tcggaaaccg tatggcgtca ggccaacaag taccgggttc
     7921 cccgtctggc attcgtgaac aagatggacc gcaccggcgc gaacttcttc aaggtctatg
     7981 accagatgcg cgcgcgcctg aaggccaatc cggttccgat ccaggtgccg atcggcgccg
     8041 aagatacgtt cgaaggcgtg gtcgacctga tcaagatgaa ggcgatctac tgggacgagg
     8101 cttcccaggg tacgaaattc gaataccgcg atatccctgc cgaactgcaa gcgaccagcg
     8161 acgaatggcg cgagaagatg gttgaggctg ccgccgaggc caacgaagag ctgatgaaca
     8221 agtacctcga agagggggag ttgtccgagg aagagatcaa gaaagccatc cgtctgcgca
     8281 ccatcgccgg tgagatcgtg ccgatgatgt gcggcacggc tttcaaaaac aagggcgtgc
     8341 aggccatgct cgacgccgtg atcgagtacc tgccggcgcc gaccgacatc ccgccggtcc
     8401 agggcacgga cgaaaacgag cagccgacgt cgcgcaaggc ggatgacaac gaaaagttct
     8461 ccgcgctcgc gttcaagatc atgaccgacc cgttcgtcgg ccagttgatc ttcttccgcg
     8521 tctattcggg cgtcgtgaat tccggtgaca ccgtatacaa ctcggtcaag ggcaagaagg
     8581 agcggctcgg ccggatcctg caaatgcacg ccaaccagcg cgaagaaatc aaggaagtgc
     8641 gcgcgggcga catcgccgct gctgtcggcc tgaaggaagc gaccaccggc gacacgctgt
     8701 gcgacccgga ttcgatcgtc acgctcgagc gcatggtgtt cccggagcct gtgatctcgc
     8761 aggcggtcgg ccttggtctt cggctcgacc gcctgcgaga tcaccggctc cgggaacacc
     8821 atgcgc
//
LOCUS       DATLGF010000089         3293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_191937, whole genome shotgun sequence.
ACCESSION   DATLGF010000089 DATLGF010000000
VERSION     DATLGF010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191937"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VM406_02740"
     CDS             <1..617
                     /locus_tag="VM406_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVL74909.1"
                     /translation="AVVRELRALDIEVHTNEQVVEVRKDGVQMASGRFIPSDIMVWAA
                     GIKVADFMKELGGLETNRINQLVVQRNLQTTRDPSIFAIGDCAACPQEDGKFVPPRAQ
                     AAHQQAKTLALSLERMISGKPLLDYTYRDYGSLVSLGDYSAVGSLMGAITRGSMFIEG
                     HVAKWMYWWLRKHHQIAVNGMFHTWLATWAELINRARNPRIKLH"
     gene            complement(695..1159)
                     /locus_tag="VM406_02745"
     CDS             complement(695..1159)
                     /locus_tag="VM406_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HVL74910.1"
                     /translation="MTTIRKQLLIGITALGLGMGSLAVHAQPSGAPGDRQAKIERMHE
                     RMQQKTEELRAKLNLTPQQEPAWNAYVAAMTPKQGERPARPSREEMAKLTAPERMDRM
                     HAMMQRAEQAMAVRAKATRDFYAVLTPEQRKVFDENFHMGRGHHGKKGMPRG"
     gene            1373..2092
                     /locus_tag="VM406_02750"
     CDS             1373..2092
                     /locus_tag="VM406_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL74911.1"
                     /translation="METPPHILIVDDDREISSLLADYLQRNGCQARTAGDGKAMQRQM
                     EQGRVDLIVLDLNLPGEDGLTLCRNLRAHSSIPVIMLTARGEPVDRILGLEMGADDYL
                     PKPFEPRELLARIRSVLRRTQALPPNLREDQPRLARFAGWTMDFTARHLVNPEGVVVA
                     LSGAEYRLLKIFIDHANRVLNRDQILNLTHGRDADPFDRSIDLQISRLRQKLGDDARS
                     PQIIKTVRNEGYVLATPVTKE"
     gene            2089..>3293
                     /locus_tag="VM406_02755"
     CDS             2089..>3293
                     /locus_tag="VM406_02755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL74912.1"
                     /translation="MKAVWQSMTSRVFLVLFAGMVITALLTAAVAMQERKRMISQFRD
                     YHAVERVTQFIVSLDTIPPELREAFYSATAGSGMRAAPASVDDAVLPDSTSLAQQLAQ
                     RLPEDYEVDSAAPLPGECAGTPGRPRRNADGRAPCEVLLVGLPDGEQMRLSVFTPRPP
                     SFVPRFDPSSAILLFLASIGVLAALVARMTMRPLKRLADAANELGKNIDRPPLPETGA
                     REIRQASAAFNAMQARIRQHVRQRTQILAAITHDLQTPLTRLRLRLEKVEDVELRDKL
                     IADLSATQVMVREGLDLARSMDNGEPLRQLDLDSLVDSICTDAADAGQDVRFEGGSGA
                     SVLARPAALRRCLENLIGNAVKYGLHAAVSVRSDGARAIVCVRDAGPGIPDEELEKVF
                     EPFYRLEDSR"
BASE COUNT          545 a         1126 c         1061 g          561 t
ORIGIN      
        1 atgcggtggt gcgcgaactg cgtgcgctcg acatcgaggt gcacacgaac gagcaggtgg
       61 tcgaggtccg caaggacggc gtgcagatgg cgagcggccg cttcattcct agcgacatca
      121 tggtctgggc ggccggcatc aaggtcgccg atttcatgaa ggaactgggc gggctggaga
      181 ccaaccgcat caaccagctg gtggtccagc gcaacctgca gaccacccgc gatccgtcca
      241 tcttcgcgat cggcgactgc gcggcctgtc cgcaggagga cggcaagttc gtcccgccgc
      301 gcgcccaggc cgcgcaccag caggccaaga cgctggcgct gtcgctggaa cgcatgatct
      361 ccggcaagcc gctgctcgac tatacctacc gcgactacgg atcactggtt tcgctcggcg
      421 attacagcgc ggtgggaagc ctgatgggcg cgatcacccg cggctcgatg ttcatcgaag
      481 ggcacgtcgc gaagtggatg tactggtggc tgcgcaagca tcaccagatt gcggtcaacg
      541 gcatgttcca cacctggctg gccacctggg cggagctgat caaccgcgcg cgcaacccgc
      601 gcatcaagct gcactgaacg taaacagcga ggcggcgcgt gcctcacctg cctcggtgaa
      661 gcgcgcgccg ccctgatcgc aaagggtggt gcgatcagcc gcgcggcatt cccttcttcc
      721 catgatggcc gcggcccatg tggaagttct cgtcgaacac cttgcgctgc tccggcgtca
      781 acaccgcgta gaagtcgcgc gttgcctttg cgcgcacggc catcgcctgc tcggcgcgct
      841 gcatcatcgc atgcatgcgg tccatgcgtt ccggcgccgt cagcttcgcc atctcttcgc
      901 ggctcgggcg ggcgggacgc tcaccctgct tcggggtcat cgccgccacg taggcgttcc
      961 acgccggctc ctgctgcggc gtcaggttca gcttggcgcg cagttcctcg gtcttctgct
     1021 gcatgcgctc atgcatgcgc tcgatcttgg cctgccggtc accgggtgcg ccggacggtt
     1081 gtgcgtgcac ggcaagcgag cccatgccga gcccgagggc ggtgataccg atcaggagtt
     1141 gcttgcgaat cgtggtcatg gtctttcctt tgcttgagag gttgcgttgc cgggtcgtct
     1201 ccccgaacgc ttgccatgat gcccgcggca cgtatcggcg gcatgtcctt cgtgtatcgc
     1261 tacgtacgcc gccgcgcccg tgtttgtatc cgcgtgtatc gcgtggcgat acggaaacct
     1321 ccggctacaa acgcggcccg cccgggccac gcggaattgc gataatgcgc ccatggaaac
     1381 ccctccccac atcctgattg tcgacgacga ccgcgagatc agctcgctgc tcgcggatta
     1441 tcttcagcgc aacggctgcc aggcgcgcac ggccggcgac ggcaaggcga tgcagcggca
     1501 gatggaacag ggtcgtgtcg acctgatcgt gctcgacctc aaccttcccg gcgaagacgg
     1561 cctgacgctg tgccgcaacc tgcgcgcgca ctccagcatc ccggtcatca tgctgaccgc
     1621 gcgcggggaa ccggtcgacc gcatcctcgg cctggaaatg ggcgccgacg actacctgcc
     1681 gaagcccttc gaaccgcgtg aactgctggc gcgcatccgc agcgtactgc gccgcacgca
     1741 ggcgctgccg ccgaatctgc gcgaggacca gccgcggctg gcccgcttcg ccggctggac
     1801 gatggatttc accgcgcgcc acctggtcaa tcccgagggc gtggtggtgg cgctgtccgg
     1861 cgccgaatac cggctgctga agatcttcat cgaccatgcg aaccgcgtgc tcaaccgcga
     1921 ccagatcctg aacctcacgc acgggcgcga cgccgatccg ttcgaccgct cgatcgacct
     1981 gcaaatctcg cggctgcgcc agaaacttgg agacgacgcg cgctcgcccc agatcatcaa
     2041 gaccgtgcgc aacgaaggct acgtgctcgc cacgccggtg accaaggaat gaaggccgtc
     2101 tggcaatcga tgaccagccg cgtgttcctg gtgctgttcg ccggcatggt gatcacggcc
     2161 ctgctgaccg cggcggtggc gatgcaggag cgcaagcgca tgatcagcca gttccgcgac
     2221 tatcacgccg tcgagcgggt gacccagttc atcgtgtcgc tggacacgat cccgccggag
     2281 ctgcgcgagg cattctatag cgccaccgcc ggcagcggca tgcgcgccgc gcccgcatcc
     2341 gtcgacgatg cggttcttcc cgatagcacc tccctcgcac agcagctcgc gcaacgcctt
     2401 cctgaggatt acgaagtgga ctcggcggcg ccgctccccg gcgaatgcgc cgggacgccg
     2461 ggccggcccc gtcgcaatgc cgacggtcgg gcgccatgcg aagtcctgct ggtcgggctt
     2521 cccgacggcg agcagatgag gctgtcggta tttacaccgc gcccgccttc attcgttccg
     2581 cgcttcgacc ccagcagcgc aatcctgctg ttcctggcca gcatcggcgt gcttgcggcc
     2641 ctggtggcgc gcatgacgat gcgtccgctg aagcgcctgg ccgatgccgc caacgagctt
     2701 ggaaagaaca tcgacaggcc accgctgcca gagaccggcg cgcgcgagat ccgccaggcg
     2761 tccgcggcct tcaacgcaat gcaggcgcgt atccgccagc acgtgcgcca gcgcacgcag
     2821 atccttgccg caatcacgca cgatctgcag acgccgctga cccggctgcg tctgcggctg
     2881 gaaaaggtgg aagacgtgga gttgcgcgac aagctgatag ccgacctgtc ggccacccag
     2941 gtcatggtgc gcgaaggtct ggatcttgca cgcagcatgg acaacggcga accgctgcgc
     3001 cagctcgacc tggactcgct ggtcgacagc atctgcaccg atgcggccga cgccggccag
     3061 gacgtgcgct tcgagggcgg cagcggcgca tccgtgctcg cgcgtcctgc ggccctgcgc
     3121 cgctgtctcg aaaacctgat cggcaatgcg gtcaagtacg gccttcacgc ggcggtgtcg
     3181 gtgcgttccg atggtgctcg cgcgatcgta tgcgtgcgcg acgccgggcc cggtattcct
     3241 gatgaagaat tggaaaaggt gttcgaaccg ttctaccgcc tcgaagattc gcg
//
LOCUS       DATLGF010000090         2244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_144740, whole genome shotgun sequence.
ACCESSION   DATLGF010000090 DATLGF010000000
VERSION     DATLGF010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144740"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VM406_02760"
     CDS             <1..814
                     /locus_tag="VM406_02760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVL74913.1"
                     /translation="TVHCAEEGPDITYIPSMGPHGPAAARNHGWRAARGEIVAFTDDD
                     TIARADWLRNGLRAFRDDVHAVWGRIVMPLSGTPTDYELDAKNLEHAEFVTANCFVRR
                     KVMEELGGFDERFRFAWREDSDLYFRLLEYRATILHEPNAVMMHPIRPASWGVSVSQV
                     KKVQFDALLYKKHPELYRRKIRSSPRWDFYLTVAALFAAVLLALIGHPVAALLAGAVW
                     AGSTARFCLQRLRRTSKSPLHIAEMVFTSMLIPPLAVFWRAVGALRYRVAFV"
     gene            896..1846
                     /locus_tag="VM406_02765"
     CDS             896..1846
                     /locus_tag="VM406_02765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014725668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVL74914.1"
                     /translation="MPQRLKILTWHTHGSYLYYLTRAPHDFYVLSKPDRPPGYSGRHG
                     HFPWGDNVHDMPIERVKETQFDCILFQDDHQYLEDQHLYLSESQRRLPKIYLEHDPPR
                     ENPTEMRHPVDDPGTLLVHVTHFNALMWDSGRTPTRVIEHGVIVPEHVRYQGDLARGL
                     VVVNNIASRGRRLGGDVFAHARERVPLDLVGMGATETGGLGEVQHAQLAEFSARYRFF
                     FNPIRYTSLGLAVIEAMMIGMPVVALATTEMATVIDNGVNGYADTDVDKLVVAMQGLL
                     DDPALARRLGDAAQACARERFSIARFASDWDRTLRDVTAH"
     gene            1874..>2244
                     /locus_tag="VM406_02770"
     CDS             1874..>2244
                     /locus_tag="VM406_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74915.1"
                     /translation="MAVSGSYFIDRAAAEKAIALAMPMIEQAMQRKEVGESGFLYIVV
                     MDPLLTPANSSFEEAILHEHAVGDRDKWDADYGAFARAKARVAWRTGMDGHLVQELRP
                     HMLTARDTVLWGSVVVAGIVVA"
BASE COUNT          380 a          760 c          721 g          383 t
ORIGIN      
        1 gaccgtgcac tgcgccgaag agggcccgga catcacgtac attccgtcga tgggcccgca
       61 tggcccggcg gccgcgcgca accacggctg gcgcgccgcg cgcggcgaga tcgttgcctt
      121 caccgacgac gacaccatcg cccgcgccga ctggctgcgc aatggcctgc gcgcgtttcg
      181 cgacgatgtg cacgcggtgt gggggcgcat cgtgatgccg ctgtccggca ccccgacgga
      241 ctatgagctc gatgcgaaga atctcgagca tgcggagttc gtcaccgcca actgcttcgt
      301 gcgccgcaag gtgatggagg aactgggcgg cttcgacgag cgcttccgtt tcgcgtggcg
      361 cgaggattcc gacctttact tccgcctgct cgaataccgc gcaaccatcc tgcacgagcc
      421 gaacgcagtc atgatgcatc cgatccgtcc cgcgtcctgg ggcgtgtcgg tgtcgcaggt
      481 gaagaaggtg cagttcgacg cgctgctcta caagaagcat ccggagctgt accggcgcaa
      541 gatccgttcg tcgccgcgct gggatttcta cctgactgtc gcggcgctgt tcgccgccgt
      601 gctgctcgcg ctgatcgggc acccggtggc agccctgctc gccggcgcag tctgggcggg
      661 atcgacggca cgcttctgcc tgcaacggct gcgccgcacc tcgaaatcgc cgctgcacat
      721 cgccgagatg gtgttcacct cgatgctgat cccgccgctg gcggtgttct ggcgcgctgt
      781 cggcgcgctc cggtaccgcg tggccttcgt ctgatgcggc acgcgggtga catcccgcaa
      841 tgcgccgaac cgaggctctg caacaatcgg gcgtgacacc atttcaccca cgcccatgcc
      901 gcaacgcttg aagatcctga cctggcacac ccacggcagc tacctgtact acctgacccg
      961 ggccccgcat gatttctacg tgctttccaa gccggaccgc ccgcccggat acagcggccg
     1021 gcacgggcac tttccctggg gcgacaatgt gcacgacatg ccgatcgagc gtgtcaagga
     1081 aacgcagttc gactgcatcc tgttccagga cgaccaccaa tacctggagg accagcatct
     1141 ctacctctcc gagtcgcagc gccggcttcc gaagatctat ctcgaacacg acccgccgcg
     1201 cgagaacccg acggagatgc gtcatccggt cgacgatccc ggcaccctgc tcgtgcacgt
     1261 cacccatttc aatgcgctga tgtgggacag cggccgcacg cccacgcgag tgatcgaaca
     1321 cggcgtgatc gtgcccgagc acgtgcgcta ccagggggac ctggcgcgcg ggctggtggt
     1381 ggtcaacaac atcgccagcc gcggccgccg cctgggcggc gacgtgtttg cgcatgcgcg
     1441 cgaacgcgtg ccgctcgatc tggtcggcat gggcgcgacc gagactggcg gactgggcga
     1501 agtgcagcac gcgcagctcg ccgaattctc ggcacgctac cgctttttct tcaatccgat
     1561 ccgctacacc agcctgggcc ttgcggtcat cgaggcgatg atgatcggca tgccggtggt
     1621 ggcgctggcc acgacagaga tggccaccgt gatcgacaac ggcgtcaacg gctacgccga
     1681 caccgacgtc gacaagctgg ttgtcgccat gcagggtctg ctcgacgatc ccgcgctggc
     1741 gcgccggctc ggagacgcag cgcaggcctg tgcgcgcgaa cgcttctcga ttgcacgctt
     1801 tgccagcgac tgggaccgca cgctgcgcga cgtgaccgca cactgattgt tcatttcact
     1861 caaggatctc gacatggccg tatcaggtag ctatttcatt gaccgtgccg ccgcggaaaa
     1921 ggcaatcgcg ctcgcgatgc cgatgatcga gcaggcgatg cagcgcaagg aagtcggcga
     1981 gagcggattt ctctacatcg tcgtgatgga tccgctgctc acaccggcca actccagctt
     2041 cgaggaagcg atcctgcacg aacacgcggt cggcgaccgc gacaaatggg acgcggacta
     2101 cggcgccttc gcgcgcgcga aggcgcgcgt ggcctggcgt accggcatgg acggccacct
     2161 cgtccaggaa ctgcgtccgc acatgctgac cgcgcgcgat acggtgctgt ggggcagcgt
     2221 ggtggtggcc ggcatcgtgg tcgc
//
LOCUS       DATLGF010000091         4088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_208123, whole genome shotgun sequence.
ACCESSION   DATLGF010000091 DATLGF010000000
VERSION     DATLGF010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208123"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..89
                     /locus_tag="VM406_02775"
                     /pseudo
     CDS             1..89
                     /locus_tag="VM406_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
     gene            complement(81..605)
                     /locus_tag="VM406_02780"
                     /pseudo
     CDS             complement(81..605)
                     /locus_tag="VM406_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835996.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
     gene            complement(605..1570)
                     /gene="msrP"
                     /locus_tag="VM406_02785"
     CDS             complement(605..1570)
                     /gene="msrP"
                     /locus_tag="VM406_02785"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HVL74916.1"
                     /translation="MQIKRRPNGIDIPYPSEITPRAVYEGRRAFLKQLAAGAALASTG
                     GLAFSQDRPAQKLSATLNPAYVLMDRPTTYKDATTYNNFYEFGTDKADPARHAGALRT
                     RPWTVAIEGEVLRPQTIDIETLLKLAPLEERVYRLRCVEGWSMVIPWIGYSLSHLMRK
                     VEPTGNARFVEFVTLADRAQMPGLRAPIIEWPYVEALRMDEAMHPLTLLTLGMYGEVL
                     PNQNGAPVRLVVPWKYGFKSAKSLVKIRFVREQPRTAWMISAPQEYGFYANVNPDVDH
                     PRWSQATERRIGEDGFFSRKRKTLMFNGYSEVDSLYAGMDLHRNY"
     gene            complement(1627..2778)
                     /gene="ccsB"
                     /locus_tag="VM406_02790"
     CDS             complement(1627..2778)
                     /gene="ccsB"
                     /locus_tag="VM406_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HVL74917.1"
                     /translation="MEISQSTAYTPTPGFFRRLSVVDWLYAAALMAGALYALARFSEH
                     MDIYEQGILLLSAPTFAWLGWQWKPLRTLAAVVAVLALGGISLYDGSLDAAEQRFFLK
                     YLLSSQSAILWMSTLFVLSTLFYIGSLATRSDTGSAIGSKMAWAAVAMGFTGLLVRWF
                     ESYLLGPDIGYIPVSNLYEVFIQFCLITTLFYLYYEERYATRQMGAFVMLIVTAAVAF
                     LLWYMLTRDAAQIQPLVPALQSWWMKIHVPANFIGYGTFAIAAMVAIAYLFKAKGVLA
                     DRLPSLEVLDDVMYKAIAIGFAFFTIATILGALWAAEAWGGYWSWDPKETWALIVWLN
                     YAAWLHMRLMKGLRGQVAAWWAIVGLFVTTFAFLGVNMFLSGLHSYGEL"
     gene            complement(2841..>4088)
                     /locus_tag="VM406_02795"
     CDS             complement(2841..>4088)
                     /locus_tag="VM406_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HVL74918.1"
                     /translation="VVHDPETGTSFPATIKVNHPLIYKGLAVYQSSFDDGGSRLKLTG
                     YSMRGSQAAPFDIEGEVGASTALGSTIGSEYTIEWSDFRAFNVENMAQAGQDLRAVDG
                     AKRFDQRLAAGIERQLGSAARVSNDRELRNVGPSLQYKVRDKTGQAREYHNYMLPVEL
                     DGVRVFLSGMRENPNDGFRYLRIPADENGTVEDWMRLRAAVADPELRAIAARQYAARA
                     IPLERDGADDLRRQLEQSALRGLEIFAGDGDVGGYLAVSRFIERVPPGEQEKAAEVFM
                     KILNGSMWELWQAARARAGLAPTPENEEQAGFLQIATNAISDATFYGAPVYLQLKEFE
                     EIKASVLQVTRSPGKNVVYLGCLLLVIGIFAMFYIRERRLWVWIKGGSDGQAHALMAL
                     TTQRKTLDFEKDFETLKSRLTQP"
BASE COUNT          786 a         1317 c         1295 g          690 t
ORIGIN      
        1 ggccgttcat cacggtcggc ttcatcgcat tcgtgctgct gattccgctt gcggcgacca
       61 gcacgaaccg gatggtgcgc cggctcggcg aaatcgctct tccccgcccg catccaccag
      121 aagtgcagca cgcccagcgg cgcgattacg tagatcaacc ggtgcaggcg ctgccagttc
      181 ctgccgagcc ggcgcaccat ccggttcgtg ctggtcgccg caagcggaat cagcagcacg
      241 aatgcgatga agccgaccgt gatgaacggc cgcttgatta cgtcgcgcca catctcggcg
      301 acatcgaaga aatggtcgaa ccagaagaag gtcaggaagt gcaggctggc gtagaagaag
      361 gcaaacaatc cgaccatgcg ccgcagctgc agcggccagg cccagccgga aagacgccgc
      421 agcggcgtca tcgtcagcgt gatgcagaga aagtacagcg tccaggcgcc ggtactgcgc
      481 gtgatcgcct cgatcggatt ggcgccgagt ccgtcggcga ccgtcagcca ggccagccgc
      541 aggaacggca gcaggcagag caggaacacg cccgctttga aggccgtaag gcgcctgccc
      601 tgcatcagta gtttcggtgg aggtccatgc ccgcgtacag cgaatcgact tcgctatagc
      661 cgttgaacat cagtgtcttg cgcttgcgcg agaagaagcc gtcctcgccg attctgcgtt
      721 cggtcgcctg cgaccagcgc gggtggtcga cgtcggggtt cacgttcgca tagaagccat
      781 actcctgcgg cgcggaaatc atccacgcgg tgcggggttg ttcgcgcaca aagcgtatct
      841 tcaccaggga cttggccgac ttgaagccgt acttccacgg caccacgagc cgtaccggcg
      901 caccgttctg gttgggcagc acctcgccgt acatgccgag cgtgagcagg gtcagcggat
      961 gcatcgcctc gtccatgcgt agcgcctcca catacggcca ttcgatgatg ggggcgcgca
     1021 agcccggcat ctgggcgcga tcggcgagtg tgacgaactc cacgaagcgc gcgttgccgg
     1081 tcggctccac tttccgcatc agatgcgaca acgagtagcc gatccacgga atgaccatcg
     1141 accagccctc gacacaacgc agccggtaga cgcgctcctc aagtggcgcg agcttgagga
     1201 gggtctcgat atcgatggtc tgcggccgca gcacctcgcc ctcgatcgcc acggtccagg
     1261 gccgcgtcct cagcgcgccg gcgtggcggg caggatcagc tttatcggtg ccgaactcat
     1321 agaaattgtt gtacgtggtc gcgtccttgt aggtggtcgg acggtccatc aacacgtagg
     1381 cggggttaag tgtcgcggac agtttctgtg cgggacgatc ctgcgagaac gcgaggccgc
     1441 cagtgctggc gagcgccgcg cctgccgcga gctgcttgag aaaggcgcgc cgtccttcat
     1501 agacggcgcg gggggtgatc tcggagggat acgggatgtc gatcccgttc ggacggcgct
     1561 tgatctgcat ggcggcacct cgcggatgaa tgactaaaca tacaccatcc gcgggtgctc
     1621 cgctgatcag agctccccgt aggaatgcag cccggagagg aacatgttga ctccaaggaa
     1681 ggcgaaggtg gtcacgaaca ggccgacgat cgcccaccag gcggcgacct gtccgcgcag
     1741 gcccttcatg aggcgcatgt gcagccacgc cgcgtagttg agccagacga tcagcgccca
     1801 ggtctccttc gggtcccagg accagtagcc gccccaggcc tcggccgccc acagggcccc
     1861 gaggatggtc gcgatggtga agaatgcaaa gccgatcgcg atcgccttgt acatgacatc
     1921 gtcgagcact tccaacgacg gcaagcggtc ggccaatacg cccttggcct tgaacagata
     1981 ggcaatcgcc accatcgcgg cgatggcgaa cgtgccatag ccgatgaaat tcgcggggac
     2041 atggatcttc atccaccagc tctgcagcgc cggcaccagc ggctggatct gcgcggcatc
     2101 acgcgtgagc atgtaccaca gcaggaaggc cactgccgcg gtgacgatca gcatcacgaa
     2161 cgcacccatc tgccgcgttg cgtagcgctc ttcgtagtag aggtaaaaca gcgtggtgat
     2221 caggcagaac tggatgaaca cttcatacag attggaaacc gggatgtaac cgatgtccgg
     2281 accgagcaga taggactcga accagcgcac cagcaggccg gtgaaaccca tcgccacggc
     2341 cgcccaagcc atcttcgagc cgatcgcact gcctgtatcc gagcgggtcg ccagcgagcc
     2401 gatgtagaac agggtcgaga gcacgaacag cgtgctcatc catagaatcg ccgactggct
     2461 cgacaatagg tacttcagga agaagcgctg ctccgctgca tccagcgatc cgtcgtacaa
     2521 cgaaatcccg cccagggcca ggaccgcgac caccgcggcc agcgtgcgca acggcttcca
     2581 ttgccagccc agccaggcga aggtgggcgc cgacagcagc aggatgccct gctcgtagat
     2641 gtccatgtgt tcgctgaagc gggccagcgc gtacagcgcg ccggccatca gggcggcggc
     2701 atacagccag tccaccacgc tcaggcggcg gaagaatccg ggggtgggcg tgtaggccgt
     2761 tgactgcgaa atttccatca ttgatctccg aaccgccgaa gcactaggct ccggcatagt
     2821 gtactttgct cgcgcagggg tcaaggctgc gtcaggcgcg acttcagggt ctcgaagtcc
     2881 ttctcgaaat cgagagtctt gcgctgcgtg gtcagcgcca tcagcgcgtg cgcctgcccg
     2941 tcgctgccac ccttgatcca gacccagagc cggcgctcgc ggatgtagaa catggcaaag
     3001 atcccgatca ccagcagcag gcaaccgaga tacacgacgt tcttgccggg cgagcgtgtc
     3061 acctgcaata ccgaggcctt gatttcctcg aactccttga gttgcagata gaccggtgcg
     3121 ccgtagaacg tggcgtccga aatcgcgttc gtcgcaatct gcaggaaacc ggcctgctcc
     3181 tcgttttcag gcgttggcgc cagaccggcg cgcgcgcgcg ccgcctgcca cagttcccac
     3241 atgctgccgt tcaggatctt catgaagacc tcggccgcct tttcctgctc gcctggaggc
     3301 acgcgctcga tgaaacgcga caccgccaga taaccgccga catcgccgtc accggcgaag
     3361 atctccagac cacgcagcgc cgactgctcg agctggcggc gcaagtcgtc ggccccatcg
     3421 cgttccagcg ggatggcccg ggccgcgtat tgccgcgcgg cgatggcgcg cagttcggga
     3481 tcggccacag cggcgcgcag gcgcatccag tcttccaccg tgccgttctc gtcggcaggt
     3541 atgcgcaggt agcggaagcc gtcgttcgga ttctcgcgca tgccgctcag gaaaacacgc
     3601 acgccgtcga gttcgaccgg cagcatgtag ttgtgatatt cgcgcgcctg cccggtcttg
     3661 tcgcgcacct tgtactgcaa gctcggaccg acgttgcgca gctcgcgatc gttggagacg
     3721 cgcgcggccg agccgagttg acgctcgatg cccgccgcta ggcgctggtc gaaccgcttg
     3781 gcgccgtcga ccgcacgcag gtcctgcccg gcctgcgcca tgttctcgac gttgaacgca
     3841 cggaaatcgg accattcgat cgtgtactcg gagccgatgg tgctgcccag cgcggtgctc
     3901 gctcccacct cgccttcgat atcgaacggc gccgcctgcg agccgcgcat cgagtagccg
     3961 gtcagcttca ggcggctgcc gccgtcgtcg aagctggatt ggtatacggc gaggcccttg
     4021 tagatcagcg ggtgattgac cttgatggtg gccggaaagc tggtgcccgt ctcggggtcg
     4081 tgcaccac
//
LOCUS       DATLGF010000092         3534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_20529,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000092 DATLGF010000000
VERSION     DATLGF010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20529"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VM406_02800"
     CDS             complement(<1..553)
                     /locus_tag="VM406_02800"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVL74919.1"
                     /translation="MDRRTVLKAAAGTAAFSMFGSAFAQDKYPSKPIRMVVPYPPGGV
                     TDLAGRVSSEILSNKYGQPVVVENRPGANALIGQQYVAGLRPDGYTLMIGGLGSQVLP
                     PATIRNLPLDIPNRFVPIAQIAEFVNILVVKGDSPINSVEELIRHAKSSKEPITYSSN
                     GVGTSAHMTSEYFAMRTGIKTLHI"
     gene            complement(669..2099)
                     /locus_tag="VM406_02805"
     CDS             complement(669..2099)
                     /locus_tag="VM406_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVL74920.1"
                     /translation="MQQNYIAGQWVDGDAVSRNINPSDLSDVIGEYAQASADQVREAI
                     RAARKASSSWAHTTAQAKADALDKIGSEILARKQELSTLLAREEGKTLAEADGEVTRA
                     VQVFKFFAGEALRVTGEKLPICRPGIEPEITREPVGVVGIITPWNFPIAIPAWKIAPA
                     LAYGNAVVFKPADLVPASAWALAEIISRAGLPEGTFNLVMGRGTTVGKTIVSDPGIDA
                     ITFTGSVNTGRMIAASAIERMAKVQMEMGGKNPLVVMDDADLAVAVECAANGAFFATG
                     QRCTASSRLLVHKNIYGKFVDALIERMGTLRVGHALQPDTKIGPVVDASQLEQNQRYV
                     EIGRNEGAKLVFGGERIKRDTDGYFFSPALFTDCDNRMKISQEEIFGPIAAVIPFGSY
                     EEALELANDTPFGLSSGICTTSLKHANHFKRHAQAGMVMINCPTAGVDAHVPFGGRKG
                     SSYGPREQGRYAAEFFTTVKTAYVAA"
     gene            complement(2117..>3534)
                     /locus_tag="VM406_02810"
     CDS             complement(2117..>3534)
                     /locus_tag="VM406_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HVL74921.1"
                     /translation="MKFRNLMSMDVEEMIRAQPMDAVVLVGGCDKTVPAQLMGAFSAG
                     KPAIQLVGGPMMTGRYQGERLGACTDCRRYWGRYRAGEVSAIEIKKIEGNLATTAGTC
                     AVMGTASTMACITETLGMLLPYTAAIPAVHADRMRAAEATGRRAVEMALEKVKPTDIV
                     NEKSIENALRMLLAISGSTNALVHLTAMAGRLGIDISLERLNELSETTPVLVDLKPTG
                     SYYMEDFNAAGGVPAVMRELRDLLHLDCMTVSGLTVGEIIERVPEWSDTNVIRPRKQP
                     LREVGGLLGLFGSLAPNGALIKRAAADEALFEHTGRAVVFDNLEDLSKRIDDPDLDVK
                     ADDVLVLRNAGPKAAAMPEAGYIPIPSKLAKQGVKDMLRISDARMSGTAYGTIVLHVS
                     PEAAMHGPLALVQNGDRIRLSLTERRLDLLVDEATLAQRRAALPPLETSSERGYSGLY
                     QRHVMQAEHGCDFDFLRKSAG"
BASE COUNT          574 a         1133 c         1136 g          691 t
ORIGIN      
        1 ggatatgcag ggtcttgatc ccggtgcgca tcgcgaagta ttcgctggtc atgtgcgccg
       61 aggtgcccac gccattgctg ctataggtga ttggttcctt cgagctcttc gcatggcgga
      121 tcaattcctc gaccgagttg atcggcgagt cgccctttac caccaggatg ttgacgaact
      181 cggcaatctg cgcaatcggc acgaagcggt tcggaatgtc gagcgggaga ttgcggatcg
      241 tcgccggcgg caatacctgg ctgccgagcc cgccaatcat cagcgtgtag ccgtcgggac
      301 gcaggccggc cacgtactgc tggccgatca gcgcgttcgc gcccggacgg ttttcgacca
      361 cgaccggctg gccgtacttg ttgctcagga tttccgacga aacgcggcca gccaaatcag
      421 tgacgccacc cggcgggtag ggcacgacca tgcggatggg ttttgacggg tacttgtcct
      481 gtgcgaaggc gctgccaaac atgctgaagg cggcggtgcc tgctgctgct ttgagtacgg
      541 tacgacgatc catggcaaat ctcctctggt tagttgcggg cgatgaactg cgtggcacta
      601 ctaatctgat aatactattt attattgtta attctgcccg ggaacccggg cggtacaacg
      661 cttttacttc aagcggcgac gtaggcggtt ttcacggtgg tgaagaactc cgccgcatac
      721 cggccctgtt cgcgcgggcc gtagctcgat cccttgcggc cgccgaacgg gacgtgggcg
      781 tccacccccg cggtcgggca attgatcatc accatgccgg cctgtgcatg acgcttgaag
      841 tggttcgcat gcttgagcga ggtggtgcag atgccggacg acaggccgaa cggcgtgtcg
      901 ttggcaagct cgagcgcttc ctcgtagctg ccgaacggga tgacggcggc gatcggtccg
      961 aagatttctt cctggctgat cttcatgcgg ttgtcgcagt cggtaaacag cgccggcgag
     1021 aagaagtatc cgtcagtgtc gcgcttgatt cgctcgccgc cgaacaccag cttcgcgcct
     1081 tcgttgcgac cgatctcgac atagcgctgg ttctgttcga gctggctggc atcgaccacc
     1141 gggccgatct tggtatccgg ctgcagcgcg tggccgacgc gcagcgttcc catgcgttcg
     1201 atcagcgcat cgacgaactt gccgtagatg ttcttgtgca ccaggagacg gctggacgcg
     1261 gtgcagcgct gcccggtcgc gaagaacgcg ccgttggcgg cgcattcaac cgcgacggcg
     1321 agatcggcat catccatcac caccagcgga ttttttccgc ccatttccat ctgcaccttg
     1381 gccatgcgct cgatggccga ggcggcgatc atgcggccgg tgttcaccga accggtgaag
     1441 gtgatcgcat cgatgcccgg atcgctgacg atggtcttgc cgaccgtcgt gccgcggccc
     1501 atcacgaggt tgaacgtgcc ttccggcagg ccggcgcggc tgatgatttc cgccagcgcc
     1561 caggcgctgg ccggcaccag gtcggccggc ttgaacacga ccgcgttgcc gtaggcgagc
     1621 gctggcgcga tcttccaggc cggaatcgcg atcgggaaat tccacggcgt gatgatgccg
     1681 accacgccaa ccggctcgcg cgtgatttcc ggttcgatgc cgggacggca gatcggcagc
     1741 ttctcgccgg tgacgcgcag cgcttcgccg gcgaagaact tgaagacctg caccgcgcgg
     1801 gtgacttcgc catcggcttc ggccagggtc ttgccttcct cgcgcgccag gagcgtgctc
     1861 agttcctgct tgcgcgccag gatttccgag ccgatcttgt cgagcgcgtc tgccttggcc
     1921 tgtgcggtag tgtgcgccca ggaggaagaa gccttgcgcg cggcgcggat cgcttcacgc
     1981 acctggtcgg cgctggcctg cgcgtattcg ccgatgacgt cggacaggtc ggaaggattg
     2041 atgttgcggc tgaccgcgtc gccatccacc cactggccgg caatatagtt ttgttgcatg
     2101 gtgttctcca agtaggctag ccggcgctct tgcgcaggaa atcaaaatcg caaccgtgtt
     2161 ccgcctgcat cacgtgccgc tgatacagcc ccgaatagcc gcgctcgctg ctcgtttcga
     2221 gcggcggcag ggcagcgcgg cgctgtgcca gcgtggcttc gtccaccagc aaatccagcc
     2281 tgcgctcggt cagcgacaga cggatccggt cgccgttctg caccagcgcc agcgggccgt
     2341 gcatcgctgc ctcgggtgag acgtgcagga cgatggtgcc gtaggcggtg ccgctcatgc
     2401 gcgcatccga aatgcgaagc atgtccttca ctccttgctt tgccagctta gacggaatcg
     2461 ggatgtagcc ggcctcgggc atggcagcgg ccttcggccc ggcattgcgc aagaccagca
     2521 cgtcgtccgc cttcacatcg agatccggat cgtcgatgcg cttggacagg tcttcgaggt
     2581 tgtcgaacac cacggcgcgg ccggtgtgct cgaacagcgc ctcgtcggca gcggcgcgct
     2641 tgatcagtgc gccgttgggg gcgaggctgc cgaacaggcc gagcaggccg cccacctcgc
     2701 gcagcggctg cttgcgcggc cggatcacgt tggtatccga ccactcgggc acgcgctcga
     2761 tgatttcgcc aaccgtcagg ccggacaccg tcatgcaatc caggtgcagc aggtcccgca
     2821 gttcgcgcat cacggcgggc acgccgcctg cggcattgaa gtcttccatg tagtacgaac
     2881 cggtcggctt caggtcgacc agcaccggcg tcgtttccga gagctcgttg agccgctcca
     2941 gcgaaatatc gatacccagg cgtcccgcca tggccgtcag gtgcaccagc gcattggtcg
     3001 agccgctgat ggccagcagc atgcgcagcg cgttctcgat cgacttctcg ttgacgatat
     3061 cggtcggctt caccttttcc agcgccattt ccaccgcccg ccggccggtt gcttcggctg
     3121 cgcgcatgcg gtcggcatgc acggccggaa tcgcggcggt gtagggcagc agcatgccga
     3181 gcgtctcggt gatgcaggcc atcgtgctgg ccgtgcccat caccgcgcag gtgccggcgg
     3241 tggttgcgag attgccctcg atcttcttga tctcgatggc cgacacttcg cctgcgcgat
     3301 agcggcccca gtagcgacgg caatcggtgc aggcaccgag acgctcgccc tggtagcggc
     3361 cggtcatcat cggcccgccc accagctgga tcgccggctt gccggccgag aaggcgccca
     3421 tcagctgcgc cggcacggtc ttgtcgcaac cgccgaccag caccaccgca tccatcggct
     3481 gggcgcggat catttcctcc acgtccatcg acatcaggtt gcggaacttc atgc
//
LOCUS       DATLGF010000093         2048 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_191985, whole genome shotgun sequence.
ACCESSION   DATLGF010000093 DATLGF010000000
VERSION     DATLGF010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191985"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VM406_02815"
     CDS             <1..862
                     /locus_tag="VM406_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVL74922.1"
                     /translation="MEKYGITNTFLFPTALKMMMKAYPSPREKFRLKLRAIMSAGEAV
                     GDAVFHWCETALGVTPNEMFGQTEMNYIVGNSARQWPARPGSMGRPYPGHRVAVIDDQ
                     GKPVAPGEIGEVALNRTDMHGHPDPIFFLGYWKNPEATRNKYSGDWCRTGDLALIDED
                     GYLWYQGRADDMFKAAGYRIGPSEIENCLVKHPAVANAAVVPKPDVERGNLVKAYVVL
                     TPGTARSAEADARLIAELQEHVRGKLAPYEYPKEIEFIDELPMTTTGKVQRRILRLQE
                     AERAAARQSA"
     gene            complement(873..1202)
                     /locus_tag="VM406_02820"
     CDS             complement(873..1202)
                     /locus_tag="VM406_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74923.1"
                     /translation="MDRPKAAIARIVLAGLLVVAGAGCEREDRTAGEPGPAERAGERL
                     DHAFSRAGEELNKMAERTGKHMEDFGRRLQNEAQQARREKEARRAEEARQAQAASEAQ
                     PGQTSGE"
     gene            complement(1276..2046)
                     /locus_tag="VM406_02825"
     CDS             complement(1276..2046)
                     /locus_tag="VM406_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HVL74924.1"
                     /translation="MCEPDPLSKTQGVAQLLAARRAGALTLDRDAILTPRAGIPGRPG
                     RPALVPPTAVPQRSVHTAEGRAALIHALAHIEFNAINLALDAIWRFPAMPPAYYDDWT
                     RVAGEEARHFELLCAHLGTLGHAYGDFPGHDSLWEMAEKTADDGLARMALVPRTLEAR
                     GLDASPAIRAKLAAAGDLAAAAILDIILHDEIGHVAIGNRWFGWLCRSKGLDPLLAYA
                     EMAQRYKAPMLRPPFNLEARRRAGFTEAELDLLPRKHA"
BASE COUNT          342 a          679 c          691 g          336 t
ORIGIN      
        1 gatggagaag tacgggatca cgaacacctt cctgttcccg accgcgctga agatgatgat
       61 gaaggcctat ccgtcaccac gcgagaagtt ccgtctcaag ctgcgtgcga tcatgagcgc
      121 cggcgaagcg gtcggcgacg cggtattcca ctggtgcgaa accgcgctcg gcgtgacgcc
      181 gaacgaaatg ttcggccaga ccgagatgaa ctacatcgtc ggcaacagcg cccgtcaatg
      241 gcctgcgagg cccggcagca tgggccgtcc atatccgggc caccgcgttg ccgtgatcga
      301 cgaccaaggc aagccggtag cgccgggcga aatcggtgag gtcgcgttga accgcaccga
      361 catgcatggc catccggacc cgattttctt ccttggctac tggaagaatc cggaggcgac
      421 ccgcaacaag tacagcggcg actggtgccg caccggcgac ctcgcgctga tcgatgaaga
      481 cggctatctc tggtaccagg gacgggcgga cgacatgttc aaggctgccg ggtaccggat
      541 cggtccgtcg gagatcgaga actgcctggt caagcacccg gccgtcgcca atgcagctgt
      601 cgtgccgaag ccggatgtgg agcgcggcaa cctggtcaag gcttatgtgg tactcacgcc
      661 aggtacagca cgcagcgcgg aagccgacgc gcgcctgatc gccgagctgc aggagcatgt
      721 gcgcggaaag ctcgcgccat acgagtaccc gaaggagatc gagttcatcg acgaattgcc
      781 gatgacgacg accggcaagg tgcagcgccg catcctccgc ctgcaggagg cggaacgcgc
      841 ggccgcccgc cagtcagcct gaaccgcggc ggctattcgc cgctggtctg cccgggctgg
      901 gcttcgctcg ccgcctgagc ctggcgggct tcctctgccc gcctggcttc cttttcccgg
      961 cgcgcttgct gggcctcgtt ctgcaggcgc cggccgaaat cctccatgtg cttgccggtc
     1021 cgctcggcca tcttgttcag ttcctcgccg gcgcgggaga acgcatgatc gagccgctcg
     1081 ccggcgcgct cggccgggcc cggctccccg gccgtgcggt cttcacgttc gcagccggcg
     1141 ccggcaacga cgagcagccc tgccagcacg atgcgggcga tcgctgcctt cggtctgtcc
     1201 atttccttcc ccgttcgctt cattccatgg cactcaggaa tgtgactgcc ggtgcgcgag
     1261 aaggttttcg acgcgttaag cgtgcttgcg cggcagcaaa tccagttcgg cttcggtgaa
     1321 accggcgcgt cggcgcgcct cgagattgaa cggggggcgc agcattggcg ccttgtagcg
     1381 ttgcgccatc tccgcatagg cgagcagcgg atcgaggcct ttgctccgac acagccagcc
     1441 gaaccagcgg ttgccgatcg cgacatgtcc gatctcgtca tgcaggatga tgtcgaggat
     1501 cgcggcggca gcgagatcgc cggcagcggc cagcttggcg cggatcgcgg gagaggcgtc
     1561 cagtccgcgc gcttccagcg tgcgcggcac cagcgccatg cgcgccagcc cgtcgtctgc
     1621 cgtcttctcg gccatctccc acaggctgtc atgtccggga aagtcgccgt acgcgtggcc
     1681 cagtgtgccc aggtgcgcgc acaggagttc gaaatgacgg gcctcttcgc cagccacccg
     1741 cgtccagtcg tcgtaatagg ccggcggcat cgcgggaaaa cgccagatcg cgtcgagcgc
     1801 gaggttgatc gcattgaact cgatgtgcgc gagcgcatgg atcagcgcgg cccgcccctc
     1861 ggccgtgtgg accgagcgct ggggaaccgc ggttggcgga accagcgcag ggcgccccgg
     1921 tcttcccgga atgccggcgc gcggcgtgag gattgcgtcg cggtccagcg tcaaggcacc
     1981 ggcgcgacgc gcggcgagca gctgcgcgac accctgggtc ttggacagcg gatcgggctc
     2041 gcacaggc
//
LOCUS       DATLGF010000094         5557 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_131033, whole genome shotgun sequence.
ACCESSION   DATLGF010000094 DATLGF010000000
VERSION     DATLGF010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131033"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1528
                     /locus_tag="VM406_02830"
     CDS             <1..1528
                     /locus_tag="VM406_02830"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HVL74925.1"
                     /translation="PSLVRRLRTGAGIVLLALPLTAVLFVLFPRMQGPLWGLPADAHS
                     ARTGLSETMEPGNIARLARSEAIAFRARFAGAPPAQSELYWRGIVMGDYDGRTWRPLE
                     MGPSRPVWVDLRGAPLEYQVTLEPHGKRWLFALDLPRAVPALDENSVLATSDLQLIAA
                     QPINTRVRYDAASHVDYLLQPNEPAAALERWLRLPAGFNPRTRELAARLRASAAGDAA
                     RVETVLRMFREQPFRYTLEPPRLGTHVVDEFLFDSRAGFCEHYAGAFVFLMREMGIPA
                     RVVTGYQGGEINPADGFMVVRQSDAHAWAEVWLRGRGWVRVDPTAAVAPERVEQNLAS
                     LIPPTAFGGLVTLDPSRSMIANQLLRLRHGWEAMNNSWNQWVLNYSPQRQRELLRRFG
                     FSDPDWRTLGLLLGVCLVLAAALTLLLMTLRQKRHDPLEALYETLCRRMARRGMARLP
                     HEGPRAYATRLVGADSSLNGQEKDAVARFLSTYEASRYGAPEHRAPLAQLKTLLAQCR
                     "
     gene            1525..2619
                     /gene="mltB"
                     /locus_tag="VM406_02835"
     CDS             1525..2619
                     /gene="mltB"
                     /locus_tag="VM406_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HVL74926.1"
                     /translation="MNRLSAITVSTAFAAALLAPYTLANQPQRAAAKKPAAPAQGARA
                     SEVNFMQWTEVGTFIDHMAGTHGFERRQLEGLFREVRHTERAIQLVMPAPPGRPKNWQ
                     AYRARFIDPVRINAGVAFWNTHADALARAEQRYGVPAEIIVAIIGVETVYGRNTGNFR
                     VVDVLTTLGFDYPEKPNREARMRFFRGELENTLLYARESGIDPLTLLGSYAGAIGWPQ
                     FMPGSIRRWAVDFDGDGKVDLRNSPVDAIGSVANFLVQHGWKPGEPIVFLTEVLQDDD
                     ERPWEMFIGQGLEAKFTLEELKNAGVNPPIDPPADMRYGLVDLQNGLQPTEYWLAANN
                     FFAITKYNRSYFYAMSVVDLARAVRVARAR"
     gene            3057..3515
                     /locus_tag="VM406_02840"
     CDS             3057..3515
                     /locus_tag="VM406_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HVL74927.1"
                     /translation="MRIDRFLTQEDAAVISRLAETLLRTNDGRVHYAEQLIDLISTSI
                     LLPETDTRSDFVSLYSNVSYRIVGSNERQNICIVCPDEANEHIACASILAPLSMAIFG
                     RTIGAIVEVELPFKRTQSVEIMDVQPTIMEDAGPSLCSPAAPSDASTIER"
     gene            3576..4487
                     /locus_tag="VM406_02845"
     CDS             3576..4487
                     /locus_tag="VM406_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVL74928.1"
                     /translation="MRRIRTILAVTDYSDAGRRAELRAAMLCAEHKAEVFEVLTVAER
                     DQDLEQALAARAHDANRIPGEDAPRAPLFGASHGQVHAGAACARTAGFGAPAYVVTRR
                     AEELRVDLTIASYQSGKVPVGKQRHADALELIRLADSPVLLVKREPRHAYRKTLLATD
                     FGDASIEAVRMCMRIAPGAHHTLFHTLGGDVDDAAVARAEDIVYLSRYRTSELAARRL
                     NELAAMLGSPREALSRVVRFGKPVAAITQFASRIDADLVAIGKTGEHGMSRSEVGIFA
                     ARLVNELDCDVLIVPGEPEGWPGGTAA"
     gene            4521..4937
                     /gene="rnk"
                     /locus_tag="VM406_02850"
     CDS             4521..4937
                     /gene="rnk"
                     /locus_tag="VM406_02850"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HVL74929.1"
                     /translation="MHTAETPRTVVASVLDRMRLVDLLGRLQTGTAAVDLLRRKLAMA
                     RLLLPQEMLPDVVTMNTRVMFLDLDTEGEYGLKLTYPEEAAMPGDISVLTPVGAALFG
                     LRVGQDIKWQGRSGRRLGVRVLKIVYQPEADGQYLV"
     gene            complement(4963..5544)
                     /locus_tag="VM406_02855"
     CDS             complement(4963..5544)
                     /locus_tag="VM406_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HVL74930.1"
                     /translation="MGLRVDVELPDDELERELETLWRRLNRPGDILHVLPCLPISHLG
                     LVFRYREADGEHYVYVEDPTRQRLAGYTVFNRLVELSRRADPHMRAPHSKFASAYQRR
                     GIAKAVYSWWLDSGRCLISGVRQSSGAHALWHSLSGQYALHYVELRAKALRWFATPAG
                     CVHADLYTRMLLLGRGQSLRHFSKATGMQLGGG"
BASE COUNT          954 a         1879 c         1786 g          938 t
ORIGIN      
        1 gccctcgctg gtgcggcgcc tgcgcaccgg ggccggcatc gtgctcctgg cgctgccgct
       61 caccgccgtg ctgttcgtgc tgtttccccg catgcaagga ccgctgtggg ggctgccggc
      121 ggacgcgcac agcgcgcgca ccggcctgtc ggaaacgatg gagccgggca atatcgcccg
      181 cctcgcgcgc tccgaggcga ttgcctttcg cgcaaggttt gccggcgccc cgcccgcgca
      241 gagcgagctg tactggcgcg gcatcgtgat gggcgactac gacggccgca cgtggcgtcc
      301 gctcgagatg ggaccgtcgc gcccggtctg ggtggacctg cggggcgcgc cgcttgaata
      361 ccaggtcacg ctcgagcccc acggcaagcg ctggctgttt gcgctcgatc tcccgcgcgc
      421 ggttcctgcg ctcgacgaaa attccgttct ggccacttcc gacctgcaac ttattgccgc
      481 gcagccgatc aatacccgcg tccgctatga cgccgcttcc catgtcgact acctgctcca
      541 gccgaacgaa ccggccgccg cgctcgaacg ctggctgcgg cttcccgccg gcttcaaccc
      601 gcgcacgcgc gaactcgccg cacgcctgcg cgcgtcggca gccggcgacg cggcgcgggt
      661 cgagacagtg ctgcgcatgt tccgcgaaca accgttccgc tacacgctgg agccgccgcg
      721 cctgggcacc catgtggtcg acgaattcct gttcgacagc cgcgccggct tctgcgagca
      781 ctatgcgggg gccttcgtgt tcctgatgcg cgaaatgggc attccggcgc gcgtggtcac
      841 cggctaccag ggcggcgaga tcaacccggc cgacggcttc atggtggtac gccagtccga
      901 cgcgcatgcc tgggccgaag tctggctgcg cggacgcggc tgggtgcggg tcgatccaac
      961 tgccgcggtc gcgcccgagc gcgtcgagca gaacctcgcc agcctgattc ccccgaccgc
     1021 attcggcgga ctggtcacgc tcgacccgtc gcgcagcatg atcgcgaacc agctgctgcg
     1081 gctgcgccac ggatgggaag cgatgaacaa ttcgtggaac cagtgggtgc tcaactattc
     1141 accgcagcgc cagcgcgagc tgctgcgccg cttcggcttt tccgaccccg actggcgcac
     1201 gctgggcctg ctgctcggcg tatgcctcgt gctggccgcc gcgctcaccc tgctcctgat
     1261 gacgctgcgc cagaagcgcc atgatccgct cgaggcgctt tacgaaacac tctgccgacg
     1321 catggctcga cgcggcatgg cgcgcctgcc gcacgaaggc ccgcgcgcct acgcaacgcg
     1381 cctcgtcggt gctgattcaa gcctgaacgg acaggaaaag gacgccgtgg cgcgctttct
     1441 gtccacctat gaagccagcc gctacggggc gccggaacat cgcgcgcccc tggcccaact
     1501 caagaccctg cttgcccaat gccgatgaat cgactttccg ccatcacggt ttcgactgcc
     1561 ttcgctgccg ccctgctcgc gccatacacc ctcgccaacc agccgcagcg cgccgccgca
     1621 aagaagccgg ccgcaccggc ccagggtgcg cgcgcctcgg aggtgaactt catgcagtgg
     1681 accgaggtgg gcaccttcat cgaccacatg gccggcaccc acggcttcga acgtcgccag
     1741 ctggagggcc tgtttcgcga agtgcgccac accgagcgcg cgatccagct ggtgatgccg
     1801 gctccgccgg gccggccgaa gaactggcag gcctaccggg cccgcttcat cgacccggtg
     1861 cgcatcaacg ccggcgtcgc gttctggaac acccatgccg acgcgctcgc gcgcgccgag
     1921 cagcgttacg gcgtgccggc cgagatcatc gtcgcgatca tcggcgtgga gaccgtctat
     1981 ggccgcaata ccggcaactt ccgcgtggtg gatgtgctga ccacgctcgg cttcgactac
     2041 ccggagaagc cgaaccgcga agcgcgcatg cgcttcttcc ggggcgaact cgagaacacg
     2101 ctgctgtatg cgcgcgaatc cggcatcgac ccgctcaccc tgctcggctc ctacgccggc
     2161 gcgatcggct ggccgcaatt catgcccggc agcatccggc gctgggccgt cgacttcgac
     2221 ggcgacggca aggtcgatct gcgcaactcc cccgtcgacg cgatcggcag cgtcgccaac
     2281 ttcctggtcc agcatggctg gaagccgggc gagcccatcg tcttcctgac cgaggtgctg
     2341 caggacgacg acgagcgccc gtgggaaatg ttcatcggcc agggcctgga ggcgaagttc
     2401 acgctggaag aactgaagaa cgccggcgtg aatcccccga tcgacccgcc ggcagacatg
     2461 cgctacggcc tcgtcgacct gcagaacggc ctgcagccga ccgaatactg gctcgccgca
     2521 aacaacttct tcgcgatcac caagtacaac cgcagctatt tctatgcgat gtcggtggtc
     2581 gatctggcgc gcgcggtgcg ggtagcgagg gcgcggtgat gaaaccgcat tcccctttgc
     2641 cccatcgagt tgacgaaaac cccccaacgt atatactcgg cttcattcag caacccaccg
     2701 taacggccac cgagcaccga cgtgaatccg tcctgcaaag tcttcaagcg caacgactct
     2761 ccccggcaaa ggggcgggtt ctgcgtggcc gcacgttatt tctctctccg ttgtaaggcc
     2821 cgctccacgc acggatcgat tgctctgcac atccgacgta tgcagcttgc tacgcgagac
     2881 ttcggctgcc tctcttcggc tcggtgagtt cgtaagagaa ggaccgctct cctcccgctc
     2941 accgagcaac ccctccagaa aagactttcg cacgcgcgcc tcgctcgcga gggcgcgagc
     3001 atttcacttt gcattgtttg gccaccaccg cgatgtggcc gggggattgt attgccatga
     3061 gaatcgatcg tttccttacc caagaggatg ccgccgtcat cagtcgcctc gcagagacgc
     3121 tgctgcgcac gaacgacggg cgggttcact acgccgagca gctgatcgat ctgatttcga
     3181 cctcgatcct gctgcccgaa acggatacgc gcagcgactt cgtttcgctc tattccaacg
     3241 tcagctaccg gatcgtgggc tcgaacgagc gccagaacat atgcatcgtc tgcccggatg
     3301 aagcgaacga gcatatcgcc tgcgcttcca tcctcgcgcc gctgtccatg gcgatcttcg
     3361 gccgcacgat aggcgcaatc gtcgaagtcg agcttccgtt caagcgcacc cagtccgtcg
     3421 agatcatgga tgtccagcct acgatcatgg aggatgccgg gccgtccctg tgctcgcctg
     3481 ccgcgccatc ggacgcatcg acgattgaac gctgaggccg cgccggctaa gcgggatctc
     3541 tttgcggctt ctcctcagac aggcaggtga atgaaatgag acggatcagg acgattctgg
     3601 cagtgacgga ttactccgac gcgggacggc gcgcggagct tcgcgcggca atgctatgcg
     3661 ccgagcacaa ggcggaggtg tttgaggtcc tcacggtggc ggagcgcgat caggacctcg
     3721 aacaggcgct ggcggcacgc gcacatgacg caaaccgcat tccgggcgaa gatgctccgc
     3781 gcgcgccttt gttcggcgca tcgcacgggc aggtgcatgc tggcgccgct tgtgcgagaa
     3841 cggcagggtt cggcgcgcct gcctatgtcg tcacgcgccg ggccgaagag ctgcgcgtcg
     3901 acctcaccat tgcgtcatac cagtcgggaa aggtgccggt cggcaagcaa aggcacgctg
     3961 atgcgctgga gctgatccgc ctggccgaca gtccggtatt gctggtgaag cgggagccgc
     4021 ggcatgcgta caggaagacg ctgcttgcga ctgatttcgg cgacgcgtcg atcgaagccg
     4081 tccgcatgtg catgcgcatc gcgccgggcg ctcaccatac gcttttccac acgcttggcg
     4141 gggacgtgga cgatgcggcc gtggcgcggg ccgaggacat cgtctatctg tcgcgctatc
     4201 ggacgagcga gctggcggcg cggcgtctga acgagcttgc ggccatgctt ggcagcccgc
     4261 gcgaggcact gtcgcgtgtg gtgcgcttcg ggaagccggt tgcggcgatt acgcagttcg
     4321 ccagccgcat cgacgcggac ctggtcgcca tcggcaagac cggggagcat ggcatgagcc
     4381 gctccgaggt aggcatcttt gcggcgcgcc ttgtgaatga actcgattgc gacgtcctga
     4441 tcgtcccggg cgaaccggaa ggctggcccg gcggcacggc cgcctgacgg gcgggcttgt
     4501 gacatgtgaa ggagctcggc atgcacacag ctgaaacacc tcggacagtg gtcgcatcgg
     4561 tgctggaccg catgcgcctt gtcgacctgc tgggccgtct gcagacgggg acggccgccg
     4621 tcgatctgct gcggcgcaag ctggcaatgg cccggctgct gctgccgcag gagatgctgc
     4681 cggatgtggt cacgatgaac acacgcgtca tgttcctgga cctggacacc gagggcgagt
     4741 atgggctgaa actgacctac cccgaagagg ccgcgatgcc tggcgatatc tcggtgctca
     4801 cgcctgtcgg tgcggccctg tttggcctgc gggttgggca ggacatcaaa tggcagggac
     4861 gcagcggaag aagactgggc gtacgtgttc tcaagatcgt gtatcaaccc gaagcggacg
     4921 gtcagtacct ggtctgatcc cagacccgcg catgtagggt cactatcctc cgcccagctg
     4981 catgcccgtt gccttggaga agtgccgcaa gctctgccca cgccccaaca gcagcatgcg
     5041 cgtatagaga tcggcatgaa cgcagcccgc gggtgtcgca aaccagcgca gcgccttggc
     5101 gcgcagctcg acgtaatgca aggcgtactg ccccgagagg gaatgccaca acgcatgggc
     5161 gccgctcgac tggcgcacgc cgctgatcag gcagcggccg ctgtcgagcc accacgagta
     5221 gaccgctttc gcaatgccgc gcctctgata cgcggacgca aacttcgaat gcggggcgcg
     5281 catgtgcgga tcggcgcgcc ggctcaactc caccaggcga ttgaagaccg tgtacccggc
     5341 aaggcgctgg cgggtcggat cttccacgta cacgtagtgt tcgccatccg cttcccggta
     5401 acggaatacc aggcccaggt gggatatcgg caggcaggga agaacatgga gaatgtcgcc
     5461 ggggcggttc aggcgccgcc acagggtttc aagctcgcgc tcgagctcat cgtcgggcag
     5521 ctccacatcg acacgaagac ccagcggcgc ggcgaac
//
LOCUS       DATLGF010000095         2962 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_131046, whole genome shotgun sequence.
ACCESSION   DATLGF010000095 DATLGF010000000
VERSION     DATLGF010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131046"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VM406_02860"
     CDS             complement(<1..711)
                     /locus_tag="VM406_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HVL74931.1"
                     /translation="MKTISSREQALALDRADELAPLREAFALPEGVIYLDGNSLGARP
                     HAAAARAQKLIAAEWGDDLIRSWNKAGWFDMPARLGDKLAPLIGAGPGEVVVTDTTSV
                     NLFKALAAALRRQACDPARAERRVIVTERSNFPTDAYIAQGLSEWLAHGYRVRMVDSP
                     EELDGALDEHAAVLMLTQVNYRTGAVHDMAALDALAHARGVLTVWDLAHSAGAGAVID
                     LRQHQHGRVLVQCAVQFLG"
     gene            complement(708..1343)
                     /gene="kynB"
                     /locus_tag="VM406_02865"
     CDS             complement(708..1343)
                     /gene="kynB"
                     /locus_tag="VM406_02865"
                     /EC_number="3.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylformamidase"
                     /protein_id="HVL74932.1"
                     /translation="MPRLFDISPTLGPALPVWPGDTPFSAEETWQMSDQCPVRVSRIT
                     MSTHSGAHCDAPSHYDPDGAAIDAVALDAYIGPCRVIACEGVRRIEPRHLQASLERLP
                     ARVLLRTYAHAPQQRWDADFACVAAETVDMLARQGVVLIGIDTPSLDPQQSKTMDAHQ
                     AVRHHGMAILEGIVLDGIAAGDYELIALPLKLQGLDASPVRAVLRELKESA"
     gene            1476..1952
                     /locus_tag="VM406_02870"
     CDS             1476..1952
                     /locus_tag="VM406_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HVL74933.1"
                     /translation="MKLDATDLKILTLLQQDGRISNQELAEKVFLSPSACLRRVRLLE
                     ESGAIAGYRAVLDPAVLGLEVDAFVQVTMRRDVERWHENFTAAMRTWPEVVGAYIITG
                     DANYLLRVRARNLKHYSAFVLEKLYKTAGVLDIRSNIVMQTIKDTPDIASDLFSAA"
     gene            complement(1958..2446)
                     /locus_tag="VM406_02875"
     CDS             complement(1958..2446)
                     /locus_tag="VM406_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74934.1"
                     /translation="MQPRTYVAYSPDRSGSVFALFYCGGGGSLHGWHVEAREPYFSSA
                     FFMMENLYSSEPLHMYRSVQDDVYGPWTIDSPPVPDRIRCPLPDAACHDLERLQSDFV
                     DDWLVFREDCRHPSVRVKPRRLGRMQRCGDAWVYATPGLDETVVQLVRRYWRLNEKLR
                     LH"
     gene            2634..>2962
                     /locus_tag="VM406_02880"
     CDS             2634..>2962
                     /locus_tag="VM406_02880"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HVL74935.1"
                     /translation="MQQVPNPMANFYQTQLEASRRLADAVFSGTEKIDRLMLEATHRA
                     IDEQLNMVQAMAGGSDVASVGMNAADRMLKRGNGAVNYQAEIMRAVAEMQTEIGRSMQ
                     VYVEQMGT"
BASE COUNT          533 a          946 c          993 g          490 t
ORIGIN      
        1 cccgaggaac tggacggcgc actggacgag cacgcggccg tgctgatgct gacgcaagtc
       61 aattaccgca ccggcgccgg ccgaatgggc gagatcccat accgtgagca cgccgcgcgc
      121 gtgcgcgagt gcatcaagtg ctgccatgtc atgcacggcg ccggtgcggt aattgacttg
      181 cgtcagcatc agcacggccg cgtgctcgtc cagtgcgccg tccagttcct cgggcgagtc
      241 gaccatgcgc acccggtagc cgtgcgcgag ccattcggac aggccttgcg cgatgtaggc
      301 gtcggtgggg aagttgctgc gctcggtgac gatcacccga cgctcggcgc gagccggatc
      361 gcaggcctgc ctgcgcagcg cggcggccag cgccttgaac aggttgaccg aggtggtgtc
      421 ggtgacgacg acttcacccg ggccggcgcc gatcaacggg gccagcttgt cgcccaggcg
      481 cgccggcatg tcgaaccagc cggccttgtt ccagctgcgg atcaggtcat cgccccattc
      541 ggcggcaatc agcttctggg cgcgggcggc tgccgcgtgc ggtcgggcgc cgagggaatt
      601 gccgtccagg tagatcacgc cttcgggcag tgcgaaggct tcgcgcaagg gcgcaagttc
      661 atcggcgcgg tccagcgcaa gcgcctgttc gcggctgctg atggttttca tgccgactcc
      721 ttcagttcgc gcagcaccgc gcgcaccggg ctcgcgtcca gtccctggag cttcaacggc
      781 agggcgatca gttcgtagtc gccggcggca atgccgtcga ggacgatacc ctcgaggatg
      841 gccatgccgt gatggcgcac ggcctgatgc gcgtccatcg tcttcgattg ttgcgggtcc
      901 agcgaagggg tgtcgatgcc gatcagcacg acgccttgac gcgcgagcat gtcgaccgtt
      961 tcagctgcga cacaggcaaa gtccgcgtcc cagcgctgct gcggcgcatg cgcgtaggtg
     1021 cgcagtagca cgcgcgccgg cagccgttcg agcgaggcct gaagatgccg cggctcgatg
     1081 cggcgcacgc cctcgcaggc tatcaccctg cacgggccga tgtaggcgtc gagcgcgacc
     1141 gcgtcgatcg ccgccccgtc cggatcatag tgcgaggggg cgtcgcaatg cgcgccgctg
     1201 tgcgtggaca tggtgatgcg cgagacgcgc accggacact ggtcagacat ctgccaggtc
     1261 tcttcggcgg aaaacggtgt gtcgccgggc cagaccggca gtgcgggtcc cagcgtgggg
     1321 ctgatatcga acaggcgtgg catggtgaac cttgcagtcg tcatgcggag aattgtatgc
     1381 gcgcggcgtc gacgatactt gccgatctgg tatcgaaaat gcgttcttta tcgcataaag
     1441 ttgcgcacac ccgtagatcc ttcgcagaac attcgatgaa actcgacgcc accgacctga
     1501 agatcctcac cctgctgcag caggacggcc gcatcagcaa ccaggagctt gccgagaaag
     1561 tgttcctctc cccgtcggcc tgcctgcgcc gtgtgcgcct gctggaggag agcggggcga
     1621 tcgccggcta ccgggccgtg ctcgaccccg ccgtgctcgg cctggaggtc gacgccttcg
     1681 tgcaggtgac gatgcggcgc gacgtcgagc gctggcacga aaactttacg gcggcgatgc
     1741 gcacctggcc cgaggtggtc ggcgcctaca tcatcaccgg cgacgcgaac tacctgctgc
     1801 gcgtgcgcgc gcgcaatctg aagcactact cggccttcgt tcttgagaag ctgtacaaga
     1861 ccgccggcgt gctcgacatc cgctccaaca tcgtgatgca gaccatcaag gacacgcccg
     1921 acatcgcgag tgatctgttt agtgcagcct gagcttttta gtgcagcctg agcttctcgt
     1981 tcagccgcca gtagcgtcgc accagctgca cgacggtttc gtccaggccg ggcgttgcgt
     2041 atacccaggc atcgccgcag cgctgcatgc ggcccagccg gcgcggcttc acccgcaccg
     2101 acggatgccg gcagtcctcg cgaaaaacca gccagtcgtc cacgaagtcc gattgcaggc
     2161 gctcgaggtc gtggcaggcg gcatccggca gcgggcagcg gatgcggtcc ggcaccgggg
     2221 gactgtcgat ggtccagggg ccgtagacgt cgtcctgcac gctgcggtac atgtgcagcg
     2281 gttccgacga atacaggttc tccatcatga agaacgccga cgagaagtag ggttcgcgcg
     2341 cctccacatg ccagccgtgc aggctgccgc cgcctccgca gtagaagagg gcgaacacgg
     2401 agccggatcg gtccggcgaa taagcaacgt aggtgcgcgg ctgcatgacg gatacggtcg
     2461 gatcaacgaa gcgcagttca ggtagaccgt gcagtgccgc gagtgttcgg gcgtgcgccc
     2521 gagtgaaaca gcatttgatc cgcctcaagc gaacgcccgt cgcgccccag caacggcggg
     2581 gattgcgtcc agaatggcgc gttgacccga ccgacagaag acaggagagc agcatgcaac
     2641 aggttccgaa tcccatggcc aatttctacc agacgcagct cgaagcctcg cggcgcctcg
     2701 cagatgccgt gttttccggc accgagaaaa tcgaccgcct catgcttgaa gcaacccatc
     2761 gcgctatcga cgaacagttg aacatggtcc aggcaatggc cggcggcagc gacgtggcca
     2821 gcgtcggcat gaacgctgcc gaccgcatgt tgaagcgcgg gaatggcgcg gtgaattacc
     2881 aggccgaaat catgcgcgcc gtggccgaga tgcagaccga gatcggacgc tcgatgcagg
     2941 tctatgtcga gcagatgggc ac
//
LOCUS       DATLGF010000096         4038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_65491,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000096 DATLGF010000000
VERSION     DATLGF010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65491"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="VM406_02885"
     CDS             <1..1027
                     /locus_tag="VM406_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HVL74936.1"
                     /translation="YDGVVFESPRYGRTGTGTTFELQTAMAALENTESCIATASGLSA
                     IAAVLLSHAVPGRQILVFEGVYGRTRTLCEKELNRLGVKVRFFSSEAELRERLEAPTT
                     LVYVEVPASLTMQMLDVEAVCHDAHAAGVPVACDSTWGTPAFFHPHALGVDISIHSAT
                     KYINGHSDLMLGLITGTYEALEPARTWCDRYGSHVAPDVCWLALRGLRTLGVRMQRHQ
                     ENAVAVATWLRGQERVKAVYFPVLPEDRGHALWKKQFSGGGGPFSFEFEACDEAAFSR
                     FIDNLQLFGLGTSWGGVDSLVMPAIPHHLRALPVQPDEGRLVRLHIGLADPQDLCADL
                     QQAFEQM"
     gene            1065..2222
                     /locus_tag="VM406_02890"
     CDS             1065..2222
                     /locus_tag="VM406_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVL74937.1"
                     /translation="MKISNRLRMAKPLPTTALHGRAEQLKARGVPVIDLAIALSNHPT
                     PQPVRERIEEGLRATAPLPYTEVTGAARLRGVLMDKLKRENAIEAGADEIIVTNGAKQ
                     AVYEALYALTDPGDTVLVFRPYWPANVAIPELLGLRTELADLPDTITSEFLAKLPPAK
                     VVILNNPHNPVGKVFTADELACLRDWIAANKMVALVDESYEQLIFEGRHISLAATCDW
                     RELGIVSIFSASQSYGMMGWRVGFAVASAAIVRAMETVQGPITAAASALTQLAAEGAF
                     SSGARTAMLEDYRVRRDMAIEMFGKASWLKMRSPHSGPYLWGDVSALTMNTIAFAEEM
                     LEQEKIAVMPGEALGVPGHIRLSFISDTADTLRAGVAGIIRFGDAYAARKA"
     gene            2296..>4038
                     /locus_tag="VM406_02895"
     CDS             2296..>4038
                     /locus_tag="VM406_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL74938.1"
                     /translation="MTFPAFSFNSLRGRLLLLVAFAIMPSVLMTVYTGWKERQHAFRM
                     AQENLQRLTNLAAVNESESLRAARQLLIGLANTPELLASDEQCAALLQATHKRNPGYA
                     NLGLIQMNGDVTCSAVPSQRPVNLADRDHFQRAISERRFIAGNYIFGRVIQQHTINLT
                     YPVIREDGQVAAVVFAAMTLPELDKFIQGINLPRGSVLITVDSQGNIISRRPAPEQWF
                     GKRVPDALWDAITRPERRPQIVRGPDGVGRLHAFSRVGSRDLSDYTVTIGLPVDDIVA
                     SAWRDQLLVWSVLALITILTLFATWVVGNNMIVARVRKLAATAQDLASGKLDARTGIP
                     HRNEEISSLAHALDDMAEALQRKEAERDRAEEELRAADRRKDEFLAMLAHELRNPLAP
                     IRTAADVLRFSQGDPERVRHTSEIIARQVDHMTGLVDDLLDVSRVTRGLVSLNRETLD
                     LRGVIAGAIEQSRSLIQDQAHTFTVDTPEEAAWVRGDRLRLIQVVTNLLNNAAKYTPR
                     QGRIHLWIKGMDGDWAVGVRDNGVGISKELLPHIFELFSQAERTPDRSQGGLGLGLAL
                     VKSLVELHGGSISVR"
BASE COUNT          703 a         1360 c         1319 g          656 t
ORIGIN      
        1 ctatgacggg gtggtcttcg agtcgccgcg ctacggccgc acgggcaccg gcacgacctt
       61 cgagctgcag acggcgatgg ccgcgctgga gaacacggaa agctgcattg cgaccgcgag
      121 cggactctcc gccattgccg cggtgctgct gtcgcatgcc gtgcccggca ggcagatcct
      181 ggtgtttgag ggcgtctacg ggcgcacgcg taccctgtgc gaaaaggaac tgaacaggct
      241 cggcgtgaaa gtgcgcttct tttcttccga ggccgagctg cgcgaacgcc tggaagcgcc
      301 gacgacgctg gtctacgtgg aagtcccggc gtcgctgacg atgcagatgc tcgacgtgga
      361 agcggtctgc cacgacgcgc acgcggcagg cgttccggtt gcctgcgatt cgacctgggg
      421 cacgccggcc ttctttcacc cgcacgcgct cggcgtggac atctcgattc actcggcaac
      481 caagtacatc aatggccatt ccgacctgat gctcgggttg atcaccggca cctatgaagc
      541 gctggaaccg gcacgcacct ggtgcgaccg ctatggctca catgtggcgc cggatgtctg
      601 ctggctcgcg ctgcgcgggc tgcgcacgct cggcgtgcgc atgcagcgcc atcaggaaaa
      661 tgcggtggca gtcgcaacct ggctgcgcgg tcaggaacgc gtgaaggcgg tgtacttccc
      721 ggtgcttccg gaggaccgcg gccatgcgct gtggaaaaag cagttcagcg gcggcggagg
      781 accgttcagc ttcgagttcg aagcctgcga cgaggcggcc ttctcgcgct tcatcgacaa
      841 cctgcagctg ttcggtctcg gcaccagctg gggcggcgtg gacagcctcg tgatgccggc
      901 gatcccgcat catctgcgcg cgctgccggt gcaaccagac gaaggccggc tggtgcgcct
      961 gcatatcggc ctggcagatc cgcaggacct gtgcgccgac ctgcaacagg cctttgagca
     1021 gatgtgagca ccgcaatgac aacaaccagg gagaggacaa gaacatgaag atttccaacc
     1081 gcctgcgcat ggccaagccg ctgcccacca ccgcgctgca cggccgcgcc gaacagctga
     1141 aggccagggg cgtgccggtc atcgatctcg cgatcgcgct gtcgaaccac cccacgccgc
     1201 agccggtgcg cgagcgcatc gaggaaggcc tgcgcgcgac cgcgccgctt ccctataccg
     1261 aagtgaccgg cgcggcgcgt ctgcgcggtg tgctgatgga caagctcaag cgcgaaaatg
     1321 cgatcgaggc cggcgccgat gaaatcatcg tcacgaatgg cgccaagcaa gcggtgtacg
     1381 aggcgctgta tgcgctgacc gatccgggcg atacggtgct cgtgttccgc ccgtactggc
     1441 cggcgaacgt cgccatcccc gagctgctcg gcctgcgcac cgaactggcc gacctgcccg
     1501 atacgatcac ttcggaattc ctggccaagc tgccgccggc gaaggtggtg atcctgaaca
     1561 atccgcacaa cccggtcggc aaggtgttca ccgccgacga gctcgcctgc ctgcgcgact
     1621 ggatcgccgc caacaagatg gtggcgctcg tcgatgaaag ctacgagcag ctgatcttcg
     1681 aaggccggca catcagcctg gccgccacct gcgactggcg cgaactcggc atcgtcagca
     1741 ttttttctgc atcgcagagc tacgggatga tgggctggcg ggtcggcttt gcggtggcgt
     1801 ccgcggctat cgtgcgcgca atggaaaccg tgcagggacc gatcaccgcc gccgcctccg
     1861 cgctcaccca gctcgctgcc gaaggcgcat tctcgtcggg cgcgcgcacc gcgatgctcg
     1921 aggactatcg cgtccggcgc gacatggcga tcgagatgtt cggcaaggca tcgtggctga
     1981 aaatgcgcag cccgcattcc ggcccttacc tgtggggcga cgtgtcggcg ctgacgatga
     2041 acacgattgc cttcgccgag gagatgctcg agcaggaaaa gattgcggtc atgcccggcg
     2101 aggcgctcgg cgtgcccggc cacatccgac tgagtttcat ttccgatacc gccgatacgc
     2161 tgcgcgccgg cgtggccggc atcatccgtt tcggcgacgc ttacgcggcg aggaaggcgt
     2221 gaggccgccg gtgtccggcc tgcgcacctg cctcggcatt ttcccgctgc ggcccggcgg
     2281 ccgcaggatt ccaccatgac gtttccagct ttcagtttca acagcctgcg cggccgcctg
     2341 ctcctgctgg tggcatttgc gatcatgcct tccgtgctga tgacggtgta caccggctgg
     2401 aaggagagac agcatgcgtt ccgcatggcg caggagaacc tgcagcggct caccaatctc
     2461 gcggcggtca acgagtccga atcactgcgc gccgcacgcc agctcctgat cgggctggcc
     2521 aacacccccg agctgctcgc gtcggacgaa cagtgcgccg cgctgctgca ggcaacccac
     2581 aagcgcaacc cggggtacgc gaatctcggg ctgatccaga tgaacggcga cgtcacctgc
     2641 agcgccgttc cttcccagcg ccccgtcaat ctcgccgatc gcgatcattt ccagcgggcc
     2701 atctccgaac gccgcttcat cgccggcaac tacatcttcg ggcgcgtaat ccagcagcac
     2761 acaatcaacc tgacctatcc ggtcatccgc gaggatggcc aagtcgcggc ggtcgtgttt
     2821 gcggcgatga cgctgcccga gctggacaaa ttcattcaag gcatcaacct gccgcgcggc
     2881 tcggtcctta tcaccgtcga ttcacagggc aacatcatct cgcgccgccc cgcgcccgag
     2941 cagtggttcg gcaagcgcgt gccggatgcg ctgtgggacg cgatcacgcg gccggagcgc
     3001 cgtccacaga tcgtgcgcgg ccccgacggc gtgggccgcc tgcacgcgtt ctcgcgggtc
     3061 ggctcgcgcg acctttccga ctacacggtc accatcgggc tgccggtgga cgacatcgtg
     3121 gcctccgcgt ggcgcgacca gctgctcgtc tggagcgtgc tcgcgctgat cacgatcctg
     3181 accctgtttg cgacctgggt ggtcggcaac aacatgatcg tcgcccgggt acgcaagctt
     3241 gccgccaccg cgcaggacct cgcctccggc aagctcgacg cgcgcaccgg cataccgcat
     3301 cgcaatgaag agatcagcag cctcgcgcac gcactcgacg acatggccga ggcgctgcaa
     3361 cgcaaggagg ccgagcgcga ccgcgcggaa gaggagttgc gcgcggcgga ccggcgcaag
     3421 gacgagtttc tggcgatgct cgcgcatgag ctgcgcaacc cccttgcgcc gatccgcacc
     3481 gccgccgatg tgctgcgctt ttcacagggc gacccggagc gtgtgcgcca taccagcgag
     3541 atcatcgcgc gccaggtcga ccacatgacc ggactggtcg acgacctgct ggacgtctcg
     3601 cgcgtcacgc gtggactggt gagcctgaat cgcgaaacgc tggacctgcg cggcgtgatc
     3661 gccggcgcga tcgagcagag ccgctcgctg atccaggacc aggcgcatac gttcacagtg
     3721 gacacgcccg aggaagccgc ctgggtgcgc ggcgaccggc tgcgcctgat ccaggtcgtc
     3781 accaacctgc tcaacaacgc cgccaagtac acgccccgtc agggccgcat tcacctgtgg
     3841 atcaagggca tggacggcga ctgggcggtc ggggtcaggg acaacggcgt gggcatcagc
     3901 aaggaattgc tgccgcacat tttcgaactg ttcagccagg ccgaacgcac accggaccgc
     3961 tcgcaaggcg gactggggct gggcctggcg ttggtgaaga gcctggtcga gctgcacggc
     4021 ggcagcatct ccgtgcgc
//
LOCUS       DATLGF010000097         3442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_131198, whole genome shotgun sequence.
ACCESSION   DATLGF010000097 DATLGF010000000
VERSION     DATLGF010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131198"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VM406_02900"
     CDS             <1..865
                     /locus_tag="VM406_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HVL74939.1"
                     /translation="ETGKEELQSSNQELQTVNDQLKLKLEEAAKANDDLNNLIASSDV
                     ATLFLDREMRILRYTPRIADIFNVIAADVGRPLRHITSRLDNPQLAEEAAGVFDTLQP
                     LEREARSRDGRHYIVRVHPYRTNDDRIAGAVMSFFDITSRRAAEQALRENEARLRTLL
                     TGFAQIFWETDAEGKVVEDSPSWRAYTGQTLEEWLGDGWLHAVHPDDRAQTLHEWREA
                     VAARRDVNTEFRLRRADGVWRRVNVRAVPLYDSDGTLLKWVGMNIDIEQRRRVGDRRG
                     HEETHSASQKD"
     gene            complement(924..2318)
                     /locus_tag="VM406_02905"
     CDS             complement(924..2318)
                     /locus_tag="VM406_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HVL74940.1"
                     /translation="MAEPIHVAVVGHTNAGKTSLLRTLTRRSSFGEVSERPGTTRHVE
                     AIDLKVEGASAVRFFDTPGLEDAVTLVDYMKALEHCPTPPDRVRAFLAGPEAHAAFEQ
                     EAKVLRQMLQVDAGIYVIDTREPVLPKFRAEIEILTWCAKPIMPVLNFVRDPHGRRDE
                     WLATLAAYNLHACVQFDAVAPFVGSERQLYQDMATLLRERRGELERVVRALETLRRER
                     QAAGCRIIADLLVSMAALRRPIAQEAFADTAQRERFVRAFRETVLRRSRAAVDDLLAV
                     YGFRGEEADAAVLPWLDGRWEDDLFNPETLRDAGRKLGTGAAVGGAIGFAADIALAGL
                     SLGAGTALGATIGGIASQGWGQFGRRLANRMRGVQELTLESEVLYVLAAQMLDLLAAL
                     ERRGHAAQGKIAASAGADAESAFRDVVGELQGARGQPAWSEGRAAAQDLRRERLVDVV
                     SGRLAPMLDARLTS"
     gene            complement(2311..>3442)
                     /locus_tag="VM406_02910"
     CDS             complement(2311..>3442)
                     /locus_tag="VM406_02910"
                     /inference="COORDINATES: protein motif:HMM:NF022518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HVL74941.1"
                     /translation="YGIAAAVVGGGRTVNAVTAFFALLGMPTLLLMLWAAMAIGGGSA
                     GGLGRVSFGNLLLRVLARLPGERNPHAPALAHAAYALLQRARLLPWAFGIVSHAVWAA
                     AFVLILAALWFAFSFQAYRLTWETTILEPGFFVRFVTVTGVIPHALGFPLPDAATLLD
                     PAAPGGDHRAWAWWLIGCAFVYGLLPRVVLALASWIAWRRGARRLHLDTAEPYYRKLL
                     ARFAEMEQPVVVDAEQPTPQEAAVMPAAGEPAGPARAVVGFELPPEQAWPPQPLEDAD
                     LIERIAGAGNERRALLEKLGRLRPRALLVVCHAASTPDRGTERFLREAAHEAGRTALL
                     LAAPEAGGAQRWQDWLRQAGLVRIDCLTDAGQAAAWLEGAHG"
BASE COUNT          576 a         1268 c         1156 g          442 t
ORIGIN      
        1 ggaaaccggc aaggaggagc tgcagtcgtc caaccaggaa ctgcagaccg tcaacgacca
       61 gctgaagctg aagctggagg aagccgccaa ggccaacgac gacctgaaca acctgatcgc
      121 ctcctccgac gtggccacgc tgttcctcga ccgcgagatg cggatcctgc gctatacgcc
      181 gcgcatcgcc gacatcttca atgtcatcgc cgccgacgtg gggcggccgc ttcggcacat
      241 caccagccgg ctggacaatc cgcagctggc cgaagaagcg gccggtgtgt tcgacacgct
      301 gcagccgctg gagcgcgagg cacgcagcag ggatggacgc cactacatcg tgcgcgtgca
      361 tccataccgc accaacgatg accgcatcgc gggcgcggtg atgagcttct tcgacatcac
      421 cagccggcgc gccgcggaac aggcgctacg cgagaacgag gcacgtctgc gcacgctgtt
      481 gaccggcttt gcgcagatat tctgggaaac cgatgccgag ggcaaggtgg tcgaggactc
      541 tccctcgtgg cgcgcctaca ccgggcagac gctggaagag tggctcggcg acggctggct
      601 ccatgccgta catccggatg accgggcaca gacgctgcac gaatggcgcg aagcagtcgc
      661 ggccaggcgc gacgtcaata ccgagttccg gctgcgccgc gccgacggcg tctggcgccg
      721 cgtcaatgtg cgcgcggtgc cgctgtacga cagcgacggc acgctgctca agtgggtggg
      781 gatgaacatc gatatcgaac aacgccggcg ggtgggggac cggcgggggc acgaggagac
      841 gcacagcgcg tcgcaaaagg attgacgaga aagggcgaga gctggctggc cgctgcgcaa
      901 gcccttgccg tgcgctccct gagtcagctc gtcaatcgcg catccagcat cggcgccagc
      961 ctcccgctga ccacgtcgac caggcgctcc ctgcgcaagt cctgcgccgc cgcgcgcccc
     1021 tcgctccacg ccggctgccc gcgcgcgccc tgcagttccc cgaccacgtc gcgaaacgca
     1081 gactccgcgt cggcaccggc gcttgccgcg atcttcccct gcgccgcatg cccgcgccgc
     1141 tcgagcgcgg ccagcaggtc gagcatctgc gcggccagca catacagcac ctcgctctcc
     1201 agcgtgagtt cctgcacgcc tcgcatccgg ttcgccagcc gccgcccgaa ctgtccccag
     1261 ccctggctgg cgatgccgcc gatcgtcgcg cctagcgcgg tgccggcgcc gagcgagagc
     1321 ccggccagcg cgatgtccgc cgcgaacccg atcgcgccgc cgaccgcagc gccggtgccg
     1381 agcttgcgcc ccgcatcgcg cagcgtttcc gggttgaaca ggtcgtcttc ccagcggccg
     1441 tccagccatg gcaggaccgc cgcgtccgct tcctcgccgc gaaagccgta gacggccagc
     1501 agatcgtcga ctgccgcgcg cgagcggcgc agcaccgttt cacggaaggc gcgcacgaag
     1561 cgctcgcgct gcgcggtatc ggcgaacgct tcctgcgcaa tcggccggcg cagcgccgcc
     1621 atcgacacca gcaggtccgc gatgatgcgg cagcccgccg cctgccgttc gcgccgcagc
     1681 gtctccagcg cgcgcaccac ccgttccagt tcgccgcgcc gctcgcgcag cagcgtcgcc
     1741 atgtcctggt acagctgccg ctccgagccg acgaagggcg cgaccgcgtc gaactgcacg
     1801 caggcgtgca ggttgtaggc ggccagcgtc gccagccact cgtccctgcg gccatgggga
     1861 tcgcgcacga agttcagcac cggcatgatc ggcttcgcgc accaggtgag gatttcgatc
     1921 tcggcccgga acttcggcag caccggctcg cgcgtgtcga tcacgtagat gccggcatcg
     1981 acctgcagca tctggcgcag caccttcgcc tcctgctcga acgccgcatg cgcctccgga
     2041 ccggcgagga aggcgcgcac ccggtccggc ggcgtggggc aatgttccag cgctttcatg
     2101 taatcgacca gcgtgaccgc atcctccagg cccggggtat cgaagaagcg cacggcgctg
     2161 gcgccctcca ccttcaggtc gatggcctcc acgtggcgcg tggtgcccgg acgctcggag
     2221 acttcgccga acgacgagcg tcgcgtgagc gtgcgcagca gcgaagtctt gccggcattg
     2281 gtgtgcccga ccacggcgac atggatcggc tcagccatgc gcaccctcga gccatgcggc
     2341 agcctggccg gcgtcggtga ggcagtcgat gcgcacaagc ccggcctgac gcagccagtc
     2401 ctgccagcgt tgcgcgccgc cggcctccgg ggcggccagc agcagcgcgg tacggcccgc
     2461 ttcgtgcgcc gcctcgcgca ggaagcgctc ggtgccgcga tccggcgtgg aggccgcatg
     2521 acagacgacc aagagcgcgc gcgggcgcag ccgccccagc ttctccagca gcgcgcggcg
     2581 ctcgttgccc gcgccggcga tgcgttcaat cagatcggca tcttcgagcg gctgcggcgg
     2641 ccatgcctgt tcgggcggca gctcgaagcc gacgaccgcc cgcgccggcc ctgccggctc
     2701 gcccgcggca ggcatcaccg ccgcttcctg cggcgtcggc tgctcggcat ccacgaccac
     2761 cggctgctcc atctcggcga agcgggcgag cagcttgcgg tagtacggct cggcggtgtc
     2821 caggtgcagc cgacgcgcgc cgcgccgcca ggcgatccag ctcgcaagcg ccagcaccac
     2881 cctcggcagc agcccgtaga cgaacgcgca gccgatcagc caccacgccc acgcacggtg
     2941 atcgccgccg ggcgcggccg gatcgagcag ggttgccgca tccggcagcg gaaaaccgag
     3001 tgcgtgcgga atgacgccgg tgacggtgac gaagcgcacg aagaaaccgg gttcgaggat
     3061 ggtggtctcc caggtcaggc ggtaggcctg gaacgaaaac gcgaaccaca gtgcggccag
     3121 gatcagcacg aaggccgcgg cccacaccgc atggctgacg atgccgaacg cccacggcag
     3181 aagccgcgcg cgctgcagca atgcgtaggc ggcatgggcg agagccggag cgtgcgggtt
     3241 gcgctcgccg ggcaggcgcg cgagcacgcg caacagcagg ttgccaaagg aaacacggcc
     3301 aagcccgcct gcactgccgc ccccgatcgc catcgccgcc cacagcatca gaagcagcgt
     3361 cggcatgccg agcagcgcga agaaagccgt caccgcgttc accgtgcgcc cgccgccgac
     3421 caccgccgcc gcgatcccgt at
//
LOCUS       DATLGF010000098         3640 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_145074, whole genome shotgun sequence.
ACCESSION   DATLGF010000098 DATLGF010000000
VERSION     DATLGF010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145074"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VM406_02915"
     CDS             complement(<1..267)
                     /locus_tag="VM406_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74942.1"
                     /translation="MQMFWNAIRAWLPALCALALAGCGGGDVRLTGDAGQGAAPLVAD
                     TFAANLTAEAQIPASTSGGQGAATVTVDPDSRRMVAVLVTSGVVG"
     gene            complement(454..1326)
                     /locus_tag="VM406_02920"
     CDS             complement(454..1326)
                     /locus_tag="VM406_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013390964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL74943.1"
                     /translation="MRNIDLESLHIFKAVVECGGISRAAAQLHRVQSNVTTRIRNLEE
                     RLGVQLFLRQGGKLVLSAEGRLLLGYAERLLNLSSEAESALRSGTPGGVLRIGTMEST
                     AAARLPPLLARYHADYPQVRIELVTGTSGALVGKVQRFEIEAAFVARPFNEAGLDTQL
                     AFVEELVLIAPRDWKGGPDEAGLRGRTLIAFSTGCSYRRILEDWLGESAIVPERVLEL
                     GSYHAIVACVAAGTGIAIVPRAVLATLREQPAIAVHPLPPKVARAETHLVWRAGHASV
                     ALGALRGQLASVSM"
     gene            1391..2554
                     /locus_tag="VM406_02925"
     CDS             1391..2554
                     /locus_tag="VM406_02925"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HVL74944.1"
                     /translation="MSSFASTSSRNPWPGTLAGLAALAVAMGIGRFAFTPVLPMMLEE
                     RLLTIAAGAWLASANYLGYLLGAVSAMFVQASAERAIRISLVVIVASTCAMALHLPIV
                     AWAGLRLLAGIASAWVLISVSAWAMDWVLRHQRPFLSSVVFCGVGTGIAAAGLLGIAI
                     QSAAHGSSAAWLALGAVAMVVTAAIWNRFGASEARAKGARPVLRWNAQSLRLVAAYGL
                     FGFGYIIPATFLPVQAKAALQGSAAFAWSWPVFGLAAALSTLTVAMWVRRIGNRRLWL
                     ACQLIMAVGVILPVASASLAAILGASLAVGGTFMVITAAALQEARQTAGPQATGLLAA
                     MTAAFATGQMLGPLTIGSQGSFDIPLAVAAAALVAGALLIVRPTRTETLQPQE"
     gene            2559..3164
                     /locus_tag="VM406_02930"
     CDS             2559..3164
                     /locus_tag="VM406_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HVL74945.1"
                     /translation="MSTPDTRHLDCMPGPERMPALAPEQMDAAQKAAADALIAGPRGA
                     VFGPFIPLMRSPELLDRLQKVGEYLRFQSSLETRINEFVMMIVSRQWTQQFEWCMHYP
                     LAVKAGIRAELLDALAEGRRPRGMAPDEEAAYDVCDELDRTRGVSDATYARAVEQFGE
                     RGVLDMLGLIGYFSTVSMVMNVARTPPMAGSTTAPLAPFPR"
     gene            complement(3209..>3640)
                     /locus_tag="VM406_02935"
     CDS             complement(3209..>3640)
                     /locus_tag="VM406_02935"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVL74946.1"
                     /translation="ADASVVGIDLSNEMLGIARERARQLAGRNIHLAAMNAEEMDFPD
                     GSFDCVAVPYVLSVTPHPEKLVAEIRRVCRKGGTILILNHFSGSRFWWFLERAVSSLA
                     NRIGFRSDFCYNEQILRYDWEVLSVKKVNLFGLSRLVAIRN"
BASE COUNT          562 a         1211 c         1245 g          622 t
ORIGIN      
        1 ccccaccacg ccgctggtga ccagcacggc caccatgcgt ctgctgtcag ggtccaccgt
       61 aacggtggcg gcaccctggc cgccgctggt gctggcggga atctgcgctt cggccgtcag
      121 gtttgcggca aacgtgtcag ccaccagcgg cgcggctcct tggccggcat cgccggtgag
      181 gcggacgtcg ccgccgccgc agccggctag cgcgagcgcg cagagggccg gcagccaggc
      241 gcgtatcgcg ttccagaaca tttgcatcgc gtactccttg ctgtgcttgc gccgcaagtc
      301 cgggcgcggc cgatgccgca gcccggctct cgcggccggg acgaacatcg cgcggatgca
      361 gcgttcgtgc cagacgggga ggggccaagc aaggcgcagt gaggcaggca gaaggaaaga
      421 attacggacg caggcggcgc cggccggcaa atcctacatg gacacgcttg ccaactgccc
      481 gcgcagcgct ccgagcgcca ccgaggcatg gccggcgcgc cagacgagat gggtttctgc
      541 gcgcgcgacc ttcggcggca gcggatggac cgcaattgcc ggctgttcgc gcagcgttgc
      601 cagcaccgcg cgtggaacga tcgcgattcc ggtgcctgcc gccacgcagg cgacgatggc
      661 gtgataggag ccgagttcaa gcacccgctc gggcacgatt gccgactcgc caagccagtc
      721 ttccaggatg cggcggtatg agcatccggt ggaaaaggcg atcagcgtgc gcccgcgcag
      781 gccggcttcg tcggggccgc ccttccagtc gcgcggcgcg atcaggacca gttcttcaac
      841 gaaagccagc tgcgtgtcca gtcccgcttc attgaacggc cgcgcaacga acgcggcttc
      901 gatttcgaaa cgctggacct tgcctaccag cgccccgctg gtgccggtca cgagttcgat
      961 gcgcacctgc ggatagtcgg cgtggtagcg ggcgagcagg ggcggcagcc gggcggcggc
     1021 ggtgctttcc atcgtgccga tgcgcaatac accacccggc gtcccgctgc gcaacgcgct
     1081 ttccgcctcg ctcgacaggt tcagcaggcg ctcggcatag ccgagcagga ggcggccctc
     1141 ggcggacaac accagcttgc cgccttggcg caggaacagc tggacgccca ggcgctcttc
     1201 caggttgcgg atgcgcgtgg tcacgttcga ctgcacccgg tgcaactggg cggccgcgcg
     1261 cgagatgccg ccgcactcga cgacggcctt gaagatgtgc agggattcca gatcaatgtt
     1321 tctcattttg agattctcct attcgaatta ttcatttttc gaaataggtg ggtcaagtta
     1381 gacttccgtc atgtcctcct ttgcctctac ttcttcccgc aatccctggc ccggcacgct
     1441 ggccggtctc gccgcgctcg ccgtagccat gggaatcggg cggttcgcct tcacgccggt
     1501 cttgccgatg atgctggagg agcgcctgct caccatcgcc gccggcgcct ggctggcttc
     1561 ggcgaactac ctcggctacc tgctcggcgc cgtctcggcc atgttcgtgc aggccagtgc
     1621 cgagcgcgca atccgcatca gcctggtcgt catcgtggcg agcacctgcg cgatggccct
     1681 gcatctgccc atcgtcgcgt gggcagggtt gcgcctgctc gccggcatcg ccagcgcgtg
     1741 ggtgctgatc tcggtgtctg cctgggcgat ggactgggtg ctgcggcatc agcgtccgtt
     1801 cctatccagc gtggtgtttt gcggcgtcgg caccggcatc gccgcggccg gcttgctcgg
     1861 cattgcgatc cagtcggccg cgcacggctc ctctgccgcc tggctggcgc tgggggccgt
     1921 ggcgatggtg gtgacggcgg caatctggaa ccggttcggc gccagcgagg cgcgcgcaaa
     1981 gggcgcgcgg ccggtgctgc gctggaacgc gcagtcgctg cgcctggtcg ccgcctacgg
     2041 cctgttcgga ttcggctaca tcatccccgc gacctttctg ccggtccagg ccaaggcggc
     2101 gctgcaggga agcgcggcgt tcgcctggtc gtggccggtg ttcggcctgg cggcggcgct
     2161 gtccacgctg accgtagcga tgtgggtgcg ccgcatcggc aaccgcaggt tgtggctggc
     2221 ctgccagctg atcatggccg tcggcgtcat ccttcccgtg gcgagcgcca gcctggccgc
     2281 gatactcggc gcctcgctgg ccgtgggcgg caccttcatg gtcattaccg ccgccgcgct
     2341 gcaggaggcg cgtcagacgg cgggaccgca ggccaccggc ctgcttgccg cgatgacggc
     2401 cgctttcgcg accggacaga tgctgggacc gctcacaatc ggctcccagg gcagcttcga
     2461 cattcccctt gcggtggcgg cggcggccct ggtggccggc gcgctcttga tcgtcaggcc
     2521 aacccgtacc gaaaccctac aaccacagga gtaagcagat gagcacacca gacacccgcc
     2581 acctcgattg catgcccgga ccggagcgca tgcccgcgct ggcgccggag cagatggacg
     2641 cggctcaaaa agccgcagcc gatgcgctga tcgccggtcc gcgcggggcg gtattcggtc
     2701 ccttcatccc gctcatgcgc agcccggaac tattggatcg cttgcagaag gtcggcgaat
     2761 acctgcgctt tcagagttcg ctggagacgc gcatcaacga gttcgtgatg atgatcgtct
     2821 cgcgccagtg gacccagcag ttcgagtggt gcatgcacta tccgctggcg gtgaaggccg
     2881 ggatccgcgc cgagctgctc gatgcgctcg ccgaaggcag gcgcccgcgc ggcatggcgc
     2941 ctgacgagga ggctgcctac gatgtctgcg atgaactcga ccgcacgcgc ggcgtgtcgg
     3001 acgccaccta tgcgcgtgca gtggaacagt ttggcgagcg cggcgtgctc gacatgctcg
     3061 gtttgatcgg ctacttcagc acggtgtcga tggtgatgaa tgtcgcgcgt acgccgccca
     3121 tggccggcag caccacggcg ccgctggcgc cgtttccgcg ttgacagagc agggaaggga
     3181 agcggcgggc cgtgatggcc cgtcggcgtc agttgcgaat cgcgaccagg cgcgacagcc
     3241 cgaacaggtt gaccttcttg accgagagca cttcccagtc gtagcgcagg atctgctcgt
     3301 tgtagcagaa gtcggaacga aagccgatgc ggttcgccag cgagctgacc gcgcgctcga
     3361 ggaaccacca gaaacggctg ccgctgaagt ggttgaggat caggatcgtt ccgcccttgc
     3421 ggcacacgcg gcgaatctcc gcaaccagtt tttccggatg cggcgtcaca gatagcacgt
     3481 acggaacggc gacgcagtcg aaactgccgt ccgggaagtc catttcctcc gcattcatcg
     3541 cggccaggtg gatattgcgg ccagccagct gacgcgcgcg ttcgcgggcg atgccgagca
     3601 tctcgttgga aaggtcgatg ccgacgacgg atgcgtcggc
//
LOCUS       DATLGF010000099         4162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_51409,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000099 DATLGF010000000
VERSION     DATLGF010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51409"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VM406_02940"
     CDS             complement(<1..373)
                     /locus_tag="VM406_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HVL74947.1"
                     /translation="MPYRPTEKTVARKAEIRQRLIDAAMEIVASKGFGGLTVSLLADE
                     AGIATGAVYKHFDSKAQICAEVFRIATEKEVNLVRETASKSGSPSERLLEAIAVFAHR
                     AIRGQRLAYALIAEPVDAAVDE"
     gene            617..2230
                     /locus_tag="VM406_02945"
     CDS             617..2230
                     /locus_tag="VM406_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVL74948.1"
                     /translation="MPLEDYNLYQQDTALREAVLREGGGWAEERLQDFGKLTGSRELI
                     ELGFQANENKPVLHTHDRHGHRVDEVRFHPAYHRLMQISLENGLHASHWRDPRPGAHV
                     ARAAMFYMMSQVEAAHCCPITMTSAAIPALRAQPELASQWEQKILALAYDPRNVPAER
                     KEAITVGMAMTEKQGGSDVRANTTRAFPLGAGGPGQAYELVGHKYFVSAPMCDVFLVL
                     AQAPGGLSCFLLPRWRPDGSKNPLQIQRLKNKMGNVANASSETELRGAHAVMIGEEGR
                     GVATILKMVAMTRFDCMIGSSAGMRQAAAQALHHCANRSAFGRLLAEQPLMQNVLADL
                     VIESEAALAMTMRIARALDASEHDGVERLLVRVGTAVGKYWICKRTPGHAYEAMECIG
                     GSGVMEDSIMPRLLRESPVNSIGEGSGNVQCLDMLRAMSQSPQSVEAFVAEVRSAAGQ
                     DTRLDRHVGKLLKELADRDDIEYRARAVVEKMALALQGALLVKHAPSEVADAFCASRL
                     NDEVSGFVYGGLPRGVDCAALVRRATPVV"
     gene            2483..3874
                     /locus_tag="VM406_02950"
     CDS             2483..3874
                     /locus_tag="VM406_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HVL74949.1"
                     /translation="MQVTATDELLRKLASAMPLSRRSFLTRTALGLTASVAAGGCRAS
                     DDKKLPASLRSPDEPIFHNSRDWEEVRGLFSLNPDVVHMSALYVASHPKPVRDAIEYY
                     RRELDTNPVVFLNQQNRRRVNEARDAAARYLGVEERDDIALTDSTTMGLGLVYNGLRL
                     GEGDEVVTTLNDYYVTHESLRLASERKGIVVRRVQLYEDIHKATEDSLTKVIVDAINP
                     RTRALALTWVHSGTGLKLPIARICNAVQRLNAKRDDAERVLVVVDGVHGFGVEDVTMP
                     GMGCDFFIAGCHKWLFGPRGTGIIWGSAQGWERMQPIVPSFHDDGTREAWMTGTDVTG
                     RTDGRRMTPGGFKPFEHQWAISEAFAMHEELGKARVQRRTHDLASQLKEGLRKMRHVS
                     LRTPMSQKLSAGIVCLEVDGMDPWTAVARLRERGIIATVTPYAERYLRFAPSIRNSPQ
                     EVEKALEGVRALA"
     gene            complement(3883..>4162)
                     /locus_tag="VM406_02955"
     CDS             complement(3883..>4162)
                     /locus_tag="VM406_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HVL74950.1"
                     /translation="AEGMRWPDMQGWVLLLSIGASALAAQFALTRAFARGNTLLAVTL
                     QYTGVLFAAGWGILLWGDVPGTASWLGMALIVVCGIASTWISSAPKRD"
BASE COUNT          749 a         1305 c         1437 g          671 t
ORIGIN      
        1 cttcatccac ggccgcgtcg accggttcgg cgatcagggc gtaggccagg cgctgcccgc
       61 ggatcgcgcg gtgggcaaac accgcaattg cctcgagcag tcgctctgac ggggagccgc
      121 tcttgctcgc ggtctcgcgc accaggttga cttccttttc ggtggcgata cgaaacacct
      181 ccgcgcagat ctgcgccttg gaatcgaagt gcttgtagac ggcgccggtc gcaatgcccg
      241 cttcgtcggc aagcaggctc acggtcagtc ctccgaagcc cttgctggcg acgatctcca
      301 tggccgcgtc gatcaggcgc tgcctgatct cggccttgcg tgcgacggtt ttctcggtgg
      361 ggcgataggg catgattgac taaacgccta tgttttggcc gggcagtgta accgagcgga
      421 atcaagccaa acaagctcgg cttgacaccc ttgaagccgc gtggtctact ggcccggcac
      481 gcgtgaatac taattcacac ctttcttgct gaatgcgcgc gcgaggccta tgctgaccat
      541 ggaggtgacc caaatgcttg atcttgcaca ggaaaatcgg tatttcgcag agacgcacga
      601 ggttcgtaac caacctgtgc cacttgagga ctacaacctg taccagcagg acacagcctt
      661 gcgcgaagcg gtgctgcgcg aaggcggggg atgggccgaa gagcgcctgc aggactttgg
      721 aaaactgacc ggttcgcgcg aactgatcga gctgggcttt caggccaacg agaacaagcc
      781 ggtgttgcat acgcatgatc gccacggaca tcgcgtcgac gaagtgcgct ttcatccggc
      841 ataccaccgg ctgatgcaga tctcgctgga aaacggcttg cacgcctccc actggcgcga
      901 tccgcgtccc ggcgcgcacg tggcgcgcgc ggcgatgttc tacatgatga gccaggtcga
      961 ggcggcgcac tgctgcccga tcacgatgac ctcggcggcg atccccgcgc tgcgcgcgca
     1021 gcctgagctg gcgtcgcagt gggaacagaa gatcctcgcg ctcgcctacg acccgcgcaa
     1081 cgtgccggcc gagcgcaagg aggcgatcac cgtcggcatg gcgatgacgg agaagcaggg
     1141 cggctcggat gtgcgcgcca acaccacgcg ggcgtttccg ctgggcgcgg gcggcccggg
     1201 ccaggcttac gagctggtcg gccacaagta cttcgtgtcc gcgccgatgt gcgatgtatt
     1261 cctggtgctg gcgcaggcgc cgggcgggct gtcctgcttc ctgctgccgc gctggcggcc
     1321 ggacggcagc aagaacccgc tgcagatcca gcgcctgaag aacaagatgg gcaacgtcgc
     1381 caacgcgtcg agcgaaaccg agctgcgcgg cgcccatgcg gtgatgatcg gcgaggaggg
     1441 gcgcggggtg gcgaccatcc tgaagatggt cgcgatgacc cgcttcgact gcatgatcgg
     1501 ttcatccgcc ggcatgcgcc aggctgcggc ccaggcgctg catcattgcg cgaaccgcag
     1561 cgcgttcgga cgcctgctgg ccgagcagcc gctgatgcag aacgtgctgg ccgatctggt
     1621 gatcgagagc gaggccgcgt tggcgatgac gatgcgtatc gcccgcgccc tcgatgccag
     1681 cgagcacgac ggggtcgaac ggctgctggt ccgggtgggc acggcggtcg ggaagtactg
     1741 gatctgcaag cgcaccccgg gccacgccta cgaggcgatg gagtgcatag gcggcagcgg
     1801 cgtgatggag gacagcatca tgccgcgcct gctgcgcgag tcgccggtca actcgatcgg
     1861 ggaaggcagc ggcaacgtgc agtgcctgga catgctgcgc gcgatgagcc agagcccgca
     1921 gtcggtcgag gccttcgtcg cagaggtgcg ttcggccgcc gggcaggata cgcggctgga
     1981 ccgccatgtc ggcaagctgc tgaaggaact ggccgaccgc gacgacatcg agtaccgggc
     2041 gcgcgccgtg gtcgagaaga tggcgctggc cctgcagggc gcgctgctgg tcaagcatgc
     2101 gccgtccgag gtggccgatg cgttctgcgc cagccggctg aacgatgagg ttagcggatt
     2161 cgtctacggc ggcttgccgc gcggcgtcga ttgcgcggcg ctggtcaggc gggcgacgcc
     2221 ggtcgtgtag acattgccga gcccggtgcc ggaagatgcc ggtcaatgtt gcgagcaggt
     2281 agagtggcat tgacggcttc cgaagcggtg agcgcaggca aaacttcgca atcgacttca
     2341 aatgtacttt gtaagccgtt gattcccaat ggcttcgtca tttgcgaacg agacgtgtgc
     2401 gtgctcgcgc cgcgttcgcc gggtatggtt tcgtcgatgc gggcgggagg cggcggcggc
     2461 caagatgctc ctggcgcgaa acatgcaagt tacggctacc gacgaactct tgcgcaagct
     2521 tgcctccgcc atgccgctgt cccgccgcag cttcctgacc cgcaccgccc ttggactgac
     2581 tgccagcgtc gccgccggcg gctgcagggc gagcgacgac aagaagcttc ccgcgagcct
     2641 gcggtcgccc gacgagccga tattccacaa ttcccgggat tgggaagaag tgcgcggact
     2701 gttcagcctg aatccggatg tggtccacat gagcgcgctg tacgtggcgt cgcatccgaa
     2761 gccggtacgc gacgcgatcg agtactaccg gcgcgagctt gacaccaacc cggtcgtgtt
     2821 cctgaaccag cagaaccggc gtcgcgtgaa tgaggctcgg gatgcggcgg cacgctacct
     2881 cggggttgag gagcgtgacg acatcgcgct gaccgacagc acgacaatgg ggctcgggct
     2941 tgtctacaac ggcctgcggc tgggcgaggg cgacgaggtc gtcaccacgc tcaacgacta
     3001 ttacgtcacc cacgaatccc tgcggctggc atccgagcgc aaaggaatcg tggtgcggcg
     3061 ggtgcagctg tacgaggaca tccacaaggc gaccgaggac agcctgacca aggtgatcgt
     3121 cgatgcgatc aatccgcgca cgcgcgcgct ggcgctgacc tgggtgcact ccggcaccgg
     3181 cctgaagctg ccgattgcgc gcatctgcaa cgcggttcaa cgcctcaacg cgaagcgaga
     3241 cgacgccgag cgggtgctgg tcgtggtcga cggcgtgcac ggcttcggag tcgaggatgt
     3301 gacgatgccg ggcatgggct gtgatttctt catcgccggc tgccacaagt ggctgttcgg
     3361 tccgcgcgga accggcatca tatggggcag cgcacagggc tgggaacgga tgcagcccat
     3421 cgtgccgagc ttccatgacg acggcacccg cgaggcgtgg atgaccggca ccgacgtcac
     3481 cggccgcacg gatggtcggc gcatgacgcc gggcggcttc aagccgttcg agcaccagtg
     3541 ggcgatcagc gaagcctttg cgatgcacga ggaactcggc aaggcgcgcg tgcagcgacg
     3601 cacccatgac ctggccagcc agttgaagga gggcctgcgc aagatgcgtc acgtgagcct
     3661 gcgtacgccg atgtcgcaga aactgtctgc cggcatcgtc tgcctcgaag tcgacgggat
     3721 ggacccatgg acggccgtcg cccgcctgcg tgagcgcggc atcatcgcga ccgtgacgcc
     3781 gtacgcggaa cggtacctgc gctttgcacc cagcatcaga aattcgccgc aggaggtcga
     3841 aaaggcgctc gagggcgtac gtgcgctcgc ctgagagggg aatcaatccc gtttcggcgc
     3901 gctcgagatc caggtcgagg cgatcccgca gacgacgatc agcgccattc ccagccatga
     3961 cgccgtgccc ggcacgtcgc cccacaggag aatcccccag ccggccgcaa acagtacacc
     4021 cgtgtactgc agggtgacgg cgagcagcgt gttgccgcgg gcaaaggcgc gggtgagggc
     4081 gaactgggcc gccagcgcgg aggcgccgat cgagagcagc agcacccagc cttgcatgtc
     4141 gggccagcgc atgccctccg cc
//
LOCUS       DATLGF010000100         9283 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_36721,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000100 DATLGF010000000
VERSION     DATLGF010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 9283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9283
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36721"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1553)
                     /locus_tag="VM406_02960"
     CDS             complement(<1..1553)
                     /locus_tag="VM406_02960"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HVL74951.1"
                     /translation="MKSPVPGGRTAPLNDASRARMQALISVALRNSVAALDGFTRRLA
                     SALSEAARTAPAPAALALRQAVVHLEREPASFHTLFADCLRQAFEQEVQLLHARTKSR
                     LRSGALDLSLDNFDAMQRKVQIDNHAQAFDRANAARLAAVHARLAQLLGREPAGLSNP
                     FRAELFLGAAASAWQRFEAEPEAQRVFLQQLGPAAFLPLDAILDAVDAELRERSAGAA
                     PMPASSPRALLEAERQALMQGGRTLDRAFAHLARTAALPPATLELLALLQPVLRMVAQ
                     ADGRFIVNPRHPGRRLVQLVVQASLAVAQGSDAARALEAALHAIAARLASDRGNAALD
                     AACKEVDAILAPLLQAVADRRDACIADATQQEREARAEKIARDEVLARLEGGTVPEFV
                     ESFLLEQWTRVLAAAQNGQIAKPAMLANLVQAMDELIWSVQAKEPHERAQLQERLPAL
                     IALLNAWLNVVKWEGAAREAFFARLARRHAELARGEAVPDARGRLQQSLDATERASEH
                     ELRRRADELA"
     gene            complement(1580..1756)
                     /locus_tag="VM406_02965"
     CDS             complement(1580..1756)
                     /locus_tag="VM406_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74952.1"
                     /translation="MTDRDEDRKERDPNPMDHPEEQPVEGDGSPKFGRLLLVLLGAIV
                     LVILITLASEAIYS"
     gene            complement(1753..2727)
                     /locus_tag="VM406_02970"
     CDS             complement(1753..2727)
                     /locus_tag="VM406_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HVL74953.1"
                     /translation="MTKAIRIAKTGGPEVMEYVDVEVGEPGPGQARVRHAAVGLNYID
                     VYFRDGTYPQPLPGALGMEGAGTVEAVGEGVTHVKPGDRVAYAARPPGAYAEARVMSA
                     AGLVRLPDSIAFETAAAMMLQGLTVQYLFRRTFPLKGGETILFHAAAGGVGLIACQWA
                     RALGVTMIGTVGSDEKAALAKAHGCTHTINYNKENFVERVKDITGGKGVPVVYDSIGK
                     DTFTGSLDCLAPLGLMVSYGNASGVVPPFSLQELASRGSLYITRPTLFSYIAKREDLE
                     AATADLFAMVESGKIRIDINQRYALADVAQAHRDLEARKTTGSTILLP"
     gene            complement(2746..3135)
                     /locus_tag="VM406_02975"
     CDS             complement(2746..3135)
                     /locus_tag="VM406_02975"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HVL74954.1"
                     /translation="MNKKRIALIAHDKKKDDMVELAFEHADILRQCLLVATGTTGKRL
                     SEEVGLTVERKLSGPFGGDLQIGAELSEGKVDCVIFLRDPMTPQPHEPDINALVRACD
                     VHNIPCATNVATAHLLLEQLVPHHPHK"
     gene            complement(3409..4188)
                     /locus_tag="VM406_02980"
     CDS             complement(3409..4188)
                     /locus_tag="VM406_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL74955.1"
                     /translation="MDLGIRGRHALVCGASKGLGRGCAQALAAEGVSVTLVARGADAL
                     EAAAEDIRKATGVAVVAVAADITTPEGRAQALAACPQPDILVTNAGGPPPGDFRDWTR
                     DDWIAALDANMLTPIELIKATVDGMIARRFGRIVNITSSAVKAPIDILGLSNGARSGL
                     TGFVAGLARKTVEHNVTINNLLPGQFETDRLKKTMGVSAAAAGKSVDEMFEQRRKLIP
                     ARRFGTAEEFGAVCAFLCSAQAGYMTGQNVLLDGGNYPGTF"
     gene            complement(4197..5108)
                     /locus_tag="VM406_02985"
     CDS             complement(4197..5108)
                     /locus_tag="VM406_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVL74956.1"
                     /translation="MSVAASTPAARQVFLGGLAIAIAGAVLFSAKAIVAKLIYRYDVD
                     AVTLIALRMLLSLPFFGAIALWQTWKAEPLAPADRWRIVVMGLLGYYLSSFLDFSGLQ
                     YISAGLERLILFLTPSFVLLISMFVLRRRITVIEWLALGTAYMGTILVFMHDVRLGGG
                     NVVLGSALVLGSALCYAIYLVMSAEILKRVGALRLVAYAMCVSTVACVVQFFLLRPPS
                     MLIQPADVYWLSLINAVFCTVFPVYLTMVAVERIGAPSTSQAGMIGPVATLFLAAAIL
                     DEPVTSIQLAGTGLVLAGIYLLSRKKV"
     gene            complement(5149..6603)
                     /locus_tag="VM406_02990"
     CDS             complement(5149..6603)
                     /locus_tag="VM406_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HVL74957.1"
                     /translation="MSKPLLIATRAQHALELLPQLANRHGCITGATGTGKTVTLQMMA
                     QQLSDIGVPVFMADVKGDLSGIARPGGDNAKVRERLAALGLPEPAWRGCPVTFWDVYG
                     ERGHPVRATITDLGPLLLARMLNLNDTQQGVLQLVFKIADDNGLLLLDIKDLRAMLQH
                     VGDNAAQFKTGYGNISPASIGAIQRGLIAIEEQGGDKFFGEPMLNIEDFMQTDADGRG
                     VVNILAADRLLHAPRLYAIFLLWMLSELFELLPEVGDVDKPKLVFFFDEAHLLFNEAP
                     KPLLQKIEQVVRLIRSKGVGVYFVTQNPLDIPDTVLGQLGNRVQHALRAFTPRDQKAV
                     QAAAQTFRQNPALDTAKAITELAVGEALVSFLDAKGAPQIVERAFIVPPASRIGPLTD
                     AERREVMAASLVAGVYDQPEDRKSAYEHLKERVEQELPKAPPAKAKETARTPASARRG
                     DSVVEAMMKSAARSIGSQIGRQIIRGVLGSILKK"
     gene            6749..7474
                     /locus_tag="VM406_02995"
     CDS             6749..7474
                     /locus_tag="VM406_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HVL74958.1"
                     /translation="MAGHSKWANIKHKKAATDAKRGKIWTRLIKEITVAAKLGGGDIN
                     ANPRLRLAVEKAADANMPKDNVQRAIQRGTGGAEGANYEEIRYEGYGINGAAIIVDCM
                     TDNRVRTVAEVRHAFSKYGGNMGTEGSVAFLFKHCGQLLFAPGTNEDALMEAALEAGA
                     EDVVTDEEGGIEVITAPHDFSVVKEALEKSGFKAEVAEVVMKPSTETVFTGDDAVKMQ
                     KLLDALENLDDVQEVYTNAVIAE"
     gene            7575..8849
                     /gene="purD"
                     /locus_tag="VM406_03000"
     CDS             7575..8849
                     /gene="purD"
                     /locus_tag="VM406_03000"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HVL74959.1"
                     /translation="MKILVVGSGGREHALAWKLAQSHRIQKVYVAPGNGGTALDPRLE
                     NVDITDPAWLAQFAIKENIALTVVGPEAPLAAGIVNIFRDQGLKIFGPSKEAAQLESS
                     KDFAKAFMKRHRIPTADYQTFSDVLAARKYIRQKGAPIVIKADGLAAGKGVVVAMTID
                     EAIDAVDSMLSQNRFGDAGARVVVEEFLEGEEASFIVMVDGKNVLPLATSQDHKRLKD
                     NDEGPNTGGMGAYSPAPIVTPSLHARIMREIINPTVLGMSKDGIPYTGFLYAGLMIDA
                     KGNPKTLEFNCRMGDPETQPIMARLKTDFVDVVEHAVDATLDQIELEWDRRTALGVVM
                     AAANYPETPRKGDVITGIPPETADSVTFHAGTTLKDGKPVTSGGRVLCVVGLGDSVRM
                     AQKNAYEVVDKIRFEGAQFRRDIGWRALKRPG"
     gene            9085..>9283
                     /locus_tag="VM406_03005"
     CDS             9085..>9283
                     /locus_tag="VM406_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HVL74960.1"
                     /translation="MDASQVKPYLLSLQQRIVTALEAIDGASFLRDEWQRAPGESPID
                     GGGITCILEDGKVLERGGVGFS"
BASE COUNT         1673 a         3041 c         3074 g         1495 t
ORIGIN      
        1 gcgagttcgt cggcgcgtcg acgcagctca tgctcgctcg cgcgctcggt cgcatccagg
       61 ctttgctgca gccgtccgcg cgcatccggc acggcctcgc cgcgcgcgag ctccgcatgg
      121 cggcgggcca accgcgcaaa gaaggcttca cgagccgccc cttcccactt caccacgttg
      181 agccaggcat tgagcagcgc gatcagggcc ggcaggcgct cctgcagctg cgcgcgctcg
      241 tgcggctcct tcgcctgcac gctccagatc agctcgtcca tcgcctgcac caggttggcg
      301 agcatggccg gcttggcgat ctggccgttc tgggcggcgg ccagcacgcg cgtccattgt
      361 tcgaggagga aggattcgac gaattccggc acggtgccgc cttccagccg cgccagcacc
      421 tcgtcgcgcg caattttctc ggcgcgcgct tcacgctcct gctgcgtcgc gtcggcgatg
      481 caggcatcac gccggtcggc gaccgcctgc agcagcggcg ccagaatcgc gtcgacttcc
      541 ttgcaggccg cgtcgagcgc ggcgttaccg cggtcgctgg caaggcgcgc ggcgatcgca
      601 tgcagcgcag cctccagcgc acgcgccgca tccgagccct gggcgaccgc aaggctcgcc
      661 tgcaccacga gctggaccag acgccggcca gggtggcgtg gattgacgat gaaacggccg
      721 tcggcctgcg cgaccatgcg caagaccggt tgcaggaggg cgagcagttc cagcgtggcc
      781 gggggcaagg cagccgtgcg cgcgaggtgg gcaaacgcac ggtccagcgt gcggccgccc
      841 tgcatcagcg cctggcgctc ggcctcaagc agcgcacggg gggaagacgc cggcatgggc
      901 gctgcgccgg cgctgcgttc gcgcagttcg gcatcgaccg cgtcgagaat ggcatccaga
      961 ggcaaaaagg cggcaggacc gagttgctgg aggaagacgc gctgcgcctc gggttcggcc
     1021 tcgaagcgct gccaggcgct ggccgctgcg cccagaaaca gttcggcccg gaagggattg
     1081 gagagtcccg ccggctcgcg cccgagcaat tgcgcaagcc gcgcatggac ggcggccagg
     1141 cgagcggcat tggcccggtc gaaggcctgc gcatggttgt cgatctggac cttgcgctgc
     1201 atcgcgtcga agttgtcgag cgagaggtcg agcgcgccgc tgcgcaggcg gctcttcgtg
     1261 cgggcatgca gcagctgcac ttcctgttca aacgcctgcc gcaggcagtc ggcgaacagc
     1321 gtgtggaacg aagcgggttc gcgctccaga tgcacgacgg cctgcctgag cgcgagcgcg
     1381 gccggcgcgg gcgccgtccg tgccgcctcg ctcagcgcgc tggcaagccg gcgcgtgaag
     1441 ccatcgaggg cggccacgct gttgcgcagc gcgaccgaga tcagcgcctg catgcgtgcg
     1501 cgcgacgcgt cgttcagagg cgcggtccgt ccgccgggaa caggtgactt catcgatgag
     1561 gcggcgccac caggggcgcc taggagtaaa tggcttcgga ggcgagtgta atcagaatca
     1621 ccagcacgat ggcgccaagc agaacgagca gcaggcgtcc gaacttcggg ctgccgtcgc
     1681 cctcgacggg ctgctcttcc ggatggtcca tcgggttggg gtcgcgttcc ttgcggtctt
     1741 cgtcgcggtc ggtcatggca gcaggatggt ggagccggtg gtcttgcgcg cctccaggtc
     1801 gcgatgcgcc tgggccacgt cggcgagcgc gtagcgctgg ttgatgtcga tcctgatctt
     1861 gccggactcg accatcgcga acagatcggc agtggcggct tccaggtctt cgcgcttcgc
     1921 gatgtacgag aacagcgtcg gccgggtgat gtacagcgaa ccgcgcgaag ccagttcctg
     1981 cagcgagaat ggcggaacga cgccggaagc attgccgtag ctgaccatca gcccgagcgg
     2041 agccaggcag tccagcgagc cggtgaaggt gtccttgccg atcgagtcgt agaccacggg
     2101 cacgcccttg ccgccggtga tgtccttgac gcgttcgacg aagttctctt tgttgtagtt
     2161 gatcgtgtgc gtgcagccgt gcgccttggc cagcgccgcc ttctcgtccg aaccgacggt
     2221 accgatcatg gtcacgccga gcgcgcgcgc ccactggcag gcgatcaggc cgacgccgcc
     2281 ggcggctgcg tggaacagga tggtctcgcc gcccttcaac gggaaagtgc ggcggaacag
     2341 gtactgcacg gtcaggccct gcagcatcat cgcggcggca gtctcgaatg cgatggaatc
     2401 cggcagcctg accaggcccg cagccgacat cacgcgtgcc tcggcatagg cgcccggcgg
     2461 gcgtgcggcg taggcaacgc ggtcgcccgg cttgacatgg gtaacgccct cgcccaccgc
     2521 ctcgacggtg ccggccccct ccatgcccag cgcgccgggc agcggctgcg ggtaggtgcc
     2581 gtcgcggaag tagacatcga tgtagttcag gccgactgcc gcgtggcgca cgcgcgcctg
     2641 gcccggcccc ggctcgccga cctcgacgtc cacgtactcc atcacttccg ggccgccggt
     2701 tttcgcgatg cggattgcct tggtcatctc gttctccttg ttgcgctatt tgtggggatg
     2761 gtggggcacc agttgctcaa gcagcaagtg agcggtggcc acgttcgttg cgcaaggaat
     2821 gttgtggacg tcgcaggcgc gaaccagcgc gttgatgtcc ggttcgtgcg gctgcggcgt
     2881 catcggatcg cgcaggaaga tcacgcagtc caccttgcct tcggacaatt ctgcaccaat
     2941 ctgcaggtcg ccgccgaagg gcccggagag cttgcgctcg actgtcaggc cgacctcctc
     3001 ggacaggcgc tttccggtgg tgccggtggc caccagcagg cactgccgca ggatgtcggc
     3061 atgttcgaac gccagttcga ccatgtcgtc cttcttcttg tcgtgcgcga tcagggcaat
     3121 gcgtttcttg ttcatggcag ggcagtggca ggaaagaaag caaggagggc aaggtcgtca
     3181 gtttaccggg ttcggcggta tccggaggtg aaatcggcaa gattgcgtcc agcgatgagg
     3241 agcggcgccg ggggccgccc tttaagccgg cgagagctcg ccttcgcccg cttgagccgc
     3301 aacgacggtg ttcaacacgg attgcaaaag attaggtttc ttttgcgagc ggaagagcgc
     3361 gaacggtaag cgggtatcga aagggaattt gctggcgacg ctccaagttc agaacgtgcc
     3421 ggggtagtta ccgccgtcaa gaagcacatt ctgcccggtc atgtagccgg cctgcgcact
     3481 gcacaaaaac gcgcataccg cgccaaactc ttccgccgtg ccgaagcgcc tggccgggat
     3541 cagctttctg cgctgctcga acatctcgtc gacactcttg ccggccgcgg ccgccgagac
     3601 gcccatcgtc tttttcaggc gatcggtctc gaactggccg ggaagcagat tgttgatcgt
     3661 gacgttgtgc tcgaccgtct tgcgcgcgag cccggcgaca aagccggtca acccggagcg
     3721 ggcgccgttg gacaggccaa ggatgtcgat tggcgccttg accgcgctgg aggtgatgtt
     3781 gacgatgcgc ccgaagcggc gcgcgatcat gccgtccacg gtcgccttga tcagctcgat
     3841 cggtgtcagc atgttcgcgt ccagcgcggc gatccagtcg tcgcgggtcc agtcgcggaa
     3901 atcgccgggc ggcggcccgc cggcattggt gaccaggatg tccggctgcg gacaggccgc
     3961 gagcgcctgc gcgcgccctt caggtgttgt aatgtcggcc gccaccgcca ccacggcgac
     4021 gccagtggcc ttgcggatgt cctcggcggc cgcttccagc gcgtcggcgc cgcgcgccac
     4081 cagcgtcacg ctcacgcctt cggccgccag cgcctgcgcg cagccgcgtc ccaatccctt
     4141 gctggcgccg cacaccagcg cgtgcctgcc gcgtataccc agatccatat ggtctcctat
     4201 acctttttgc gagaaagcag ataaatgccg gccagcacca ggccggtgcc ggcgagctgg
     4261 atgctggtga cgggttcgtc gaggatcgcc gcggccagga acagcgtggc gaccggcccg
     4321 atcatgccgg cctgcgaagt ggatggcgcg ccgatgcgtt cgaccgccac catcgtcagg
     4381 taaaccggga acaccgtgca gaacaccgcg ttgatcagcg acagccaata cacgtcggcc
     4441 ggctggatga gcatcgaagg cggccgcagc aggaagaact ggacgacgca ggcaaccgtc
     4501 gacacgcaca tcgcataagc aaccagccgc agcgcaccga cgcgtttaag tatctcggcc
     4561 gacatcacca gatagatcgc atagcacagc gcactgccga gcacgagcgc gctgcccagc
     4621 acaacattgc cgccgcccag ccgcacgtcg tgcatgaata ccagtatcgt gcccatgtag
     4681 gcagtgccga gcgccagcca ttcgatgacg gtgatgcggc gcctgagcac gaacatcgag
     4741 atcagcagca cgaacgaagg cgtcaggaac agaatcaggc gttccaggcc ggccgaaatg
     4801 tactgcaacc cgctgaaatc gagaaagctg gaaaggtagt agccgagcag gcccatcacg
     4861 acgatgcgcc agcggtcggc cggcgccagc ggctcagcct tccaggtctg ccatagcgcg
     4921 atcgcgccga agaacggcag cgacaacagc atgcgcagcg caatcagcgt gacggcgtcg
     4981 acgtcgtagc ggtagatcag cttggcgacg atcgccttgg ccgagaacag cacggcgccg
     5041 gcgatcgcaa tggcgagccc gccgaggaag acctggcggg cagcgggggt ggaggcagca
     5101 acagacatcg gccgattgta cgacggcctg aaaattctga ctttccgcct atttcttcag
     5161 gatcgaacca agcacgccac gaatgatctg ccgcccgatc tgcgaaccga tgctgcgcgc
     5221 agccgacttc atcatcgcct ccaccaccga gtcgccgcgg cgtgcggagg cgggggtgcg
     5281 cgccgtttcc tttgccttcg ccggcggcgc cttcggcagt tcctgctcga cgcgctcctt
     5341 cagatgctcg taagcggact tgcgatcttc cggctggtcg tacacgccgg ccaccagcga
     5401 agccgccatc acctcccggc gttcggcgtc ggtgagcggc ccgatgcgcg aggcgggcgg
     5461 cacgatgaag gcgcgctcga cgatctgcgg cgcacccttc gcatccagaa aggacaccag
     5521 cgcctcgccg accgcgagct cggtaatcgc cttggcggta tcgagggccg gattctggcg
     5581 gaacgtctgc gcggccgcct gcaccgcctt ctggtcgcgc ggggtaaatg cgcgcagtgc
     5641 gtgctgcacg cggttgccga gctggccgag cacggtgtcg ggaatgtcga gcgggttctg
     5701 ggtgacgaag tacacgccca cgcccttgga gcggatcagg cgcaccacct gctcgatctt
     5761 ctgcaacagc ggcttcggcg cttcgttgaa cagcaggtgc gcctcgtcga agaagaacac
     5821 cagcttgggc ttgtcgacat cgccgacttc tggcagcagc tcgaacagct cggacagcat
     5881 ccacaacagg aagatcgcat acagccgcgg cgcatggagc aggcggtcgg ccgcgaggat
     5941 gttcaccacg ccgcgtccgt cggcgtcggt ctgcatgaag tcctcgatgt tgagcatcgg
     6001 ctcgccgaag aatttgtcgc cgccctgctc ctcgatcgcg atcaagccgc gctggatcgc
     6061 gccgatcgaa gccggcgaga tattgccgta cccggtcttg aactgcgcgg cgttgtcccc
     6121 cacgtgctgc agcattgcgc gcaggtcctt gatgtcgagc agcagcaggc cgttgtcgtc
     6181 ggcgatcttg aacaccagtt gcagcacacc ctgctgggtg tcgttcaggt tcagcatgcg
     6241 cgccagcagc agcgggccga ggtcggtgat ggtcgcgcgc accgggtggc cgcgctcgcc
     6301 gtacacgtcc cagaaggtga ccgggcagcc gcgccacgcc ggctcgggca agcccagcgc
     6361 ggccagccgc tcgcgcacct tggcattgtc gccgccgggc ctggcgatgc ccgacagatc
     6421 gcccttgaca tctgccatga acaccggcac gccgatgtcg gacaattgct gggccatcat
     6481 ctggagggtg acggttttgc cggtcccggt cgcgccggtg atgcagccgt gccggttggc
     6541 cagttgcggc aggagttcga gcgcgtgttg cgcgcgcgtc gcaatcagta gcggttttga
     6601 catgttttca aggagatagc ggcgattttg gcaagggccg gtatgttaaa atccccggtc
     6661 gatttttcgg cggtgcccgg ctattctgga ccaaagccgt ccgccccgca gcagcgcttc
     6721 cctcctcatt cccaggaaag acatctccat ggctggacac agtaaatggg ccaacatcaa
     6781 gcataagaag gcggcgaccg atgccaagcg cggcaagatc tggacccgcc tgatcaagga
     6841 aatcacggtt gcggccaagc tgggcggcgg cgacatcaac gccaacccgc gcctgcgcct
     6901 ggccgttgaa aaggcggccg acgccaacat gccgaaggac aacgtgcagc gtgcaatcca
     6961 gcgcggcacc ggcggcgcgg aaggcgcgaa ctacgaggaa atccgctacg aaggctacgg
     7021 catcaacggg gccgcgatca tcgtcgattg catgaccgac aaccgcgtgc gcacggtggc
     7081 cgaagtgcgc cacgcattct ccaagtacgg cggcaacatg ggcaccgaag ggtcggtggc
     7141 cttcctattc aagcattgcg gccagctgct gttcgcgccc ggcaccaatg aagatgcgct
     7201 gatggaagcg gcgctcgaag ccggggccga ggacgtggtg accgacgagg aaggcggcat
     7261 cgaagtcatc accgcgccgc acgatttctc ggtcgtgaag gaagcgctgg aaaaatccgg
     7321 cttcaaggcc gaagtggccg aagtcgtgat gaagccatcc accgaaaccg tcttcaccgg
     7381 cgacgacgcg gtgaagatgc aaaagctcct ggacgcgctc gagaatctcg acgatgtgca
     7441 ggaggtctac accaacgcgg tgattgcaga gtaagttccc ttgaaccacg gcggattgac
     7501 atgttccgat taccgccgtg cctcgccaca ttcgccgcgt tcaccgcggc taaccgcttt
     7561 ttttgttgaa catcatgaag atcctggtag tcggctcggg tggccgtgaa cacgcactgg
     7621 cctggaagct ggcccaatcg caccgcattc agaaagtcta tgtcgcaccc ggcaacggcg
     7681 gcaccgcgct cgacccgcgg ctggagaacg tcgacatcac cgatccggcc tggctcgccc
     7741 agttcgcgat caaggaaaac atcgcgctca cagtcgtcgg ccccgaggcg ccgctggccg
     7801 ccggcatcgt caacatcttc cgcgaccagg gcctgaagat cttcggcccg agcaaggaag
     7861 ccgcgcagct ggaaagctcg aaggacttcg cgaaggcctt catgaagcgc caccgcatcc
     7921 cgaccgccga ctatcagacc ttctccgatg tgctggccgc gcgcaaatac atccgccaga
     7981 agggcgcgcc catcgtgatc aaggccgacg gcctggccgc cggcaagggc gtggtggtcg
     8041 cgatgacgat cgacgaggcg atcgatgcgg tcgattcgat gctgtcgcag aaccgcttcg
     8101 gcgacgccgg tgcgcgcgtg gtggtcgagg aattcctaga aggcgaggaa gcgagcttca
     8161 tcgtgatggt cgacggcaag aacgtgctgc cgctggccac cagccaggac cacaagcgcc
     8221 tgaaggacaa tgacgaaggc ccgaacaccg gcggcatggg cgcgtactcg cccgcgccca
     8281 tcgtcacgcc ttcgctgcac gcgcgcatca tgcgcgagat catcaacccg accgtgctgg
     8341 gcatgtccaa ggacggcatt ccgtacaccg gcttcctcta tgccggcctg atgatcgacg
     8401 ccaagggcaa tccaaagacg ctcgaattca actgccgcat gggcgacccg gaaacccagc
     8461 cgatcatggc gcggctaaag accgatttcg tcgacgtggt cgaacacgcg gttgatgcca
     8521 cactcgacca gatcgaactg gaatgggacc gccgcaccgc gctcggcgtg gtgatggccg
     8581 cggccaatta ccctgagacg ccgcgcaagg gcgatgtgat caccggcatt ccgcccgaga
     8641 cggcggacag tgtgaccttc catgccggca ccacgctcaa ggacggcaag ccggtcacct
     8701 cgggcggccg cgtgctgtgc gtggtgggcc tgggcgacag cgtgcgcatg gcgcagaaga
     8761 acgcctacga ggtggtcgac aagatccgtt tcgaaggcgc gcaattccgg cgggatatcg
     8821 ggtggcgggc gctgaagcgg ccggggtgag cccggcgcat cggcaaagct gctaacctct
     8881 caatcatcca ctggcccagc cccttccccc ttccaggggg aaggcttgga taaagcggcc
     8941 cgcggtgggg tgcgaagccc gatccagcgc catgcttaat tgacctccgt accccctccc
     9001 taaccctccc cctggaaggg ggagggtcca ttccgcggca taaccagcca ctgccaaggc
     9061 gagcgtaatc aacgagcggc atacatggac gcatcccaag tcaaacccta cctcctctcc
     9121 ctgcagcaac gcatcgtcac cgccctcgaa gcgatcgacg gcgcaagctt cctgcgcgac
     9181 gagtggcaac gcgcgcccgg cgaaagcccg atcgacggcg gcggcatcac ctgcatcctc
     9241 gaagacggca aggtgctcga gcgcggcggc gtcggcttct cgc
//
LOCUS       DATLGF010000101         2898 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_224194, whole genome shotgun sequence.
ACCESSION   DATLGF010000101 DATLGF010000000
VERSION     DATLGF010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224194"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VM406_03010"
     CDS             <1..628
                     /locus_tag="VM406_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HVL74961.1"
                     /translation="PDSTGTEIDLSATEAMLRMLDPLAAEYEKSGVNRQRAGNRATYT
                     APSNMYKTGDGAYVTIVGSSSVIFRRLCQAMGRAELADDARFASNRSRMENVDAIDGE
                     IAGWCGSRTYDEVAVALTEFAVPFSKIYSAADIVADPHFIARGAVIRLPDAEHGSLPA
                     PCVVPRFAGYQPEPPRTGPAPGEHNEEVFGALGLCAETLAQLRARGVV"
     gene            682..1485
                     /locus_tag="VM406_03015"
     CDS             682..1485
                     /locus_tag="VM406_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008163954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HVL74962.1"
                     /translation="MEEIRKGLAPEEDDPQFARTLARGIDILLCYRVGESVLGNKDFA
                     ARTGLSKTTVARLMHTLAQLGYLRHDPKLAKYRLGAPVLSMGYPLLASMQIRQLARPL
                     MRELAERTGGAVSLGMRDRTSMIYVETSRSTDRLVRTPDIGAPIPILTTAMGRAWLCK
                     ADARERDEVLNRIRLNDPEHFERHYPAAMQAVREFGQRKYCRNRGEWRGDVYGFAVPL
                     ARSFQSQQFVVNCGIPAAAHASAAAAEKEVAPQLISLVQSLQITLGMTS"
     gene            complement(1511..2272)
                     /locus_tag="VM406_03020"
     CDS             complement(1511..2272)
                     /locus_tag="VM406_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HVL74963.1"
                     /translation="MRHPDPSFATTLAHGLALLQCFRVDAPTLSNKELSDATGLSKST
                     ITRLTYTLMSRGLLAYDAHTRRYQLGPTALSLGYPLLAHLRVRQIARPLMQQLADFAE
                     GSVSLGMRDRLNVVYIETSRGHESIEFRPDIGASLPILASAMGRAWLAAAPPGQRDDV
                     IEAFAQGNRSLRTRARKVIATAVADLETKGFCVSHGDWQPDIHALAIPLRKPVDDEVV
                     VLNCGVLTRRLKGEAIERRLGERLIETARRIEMAL"
     gene            2428..>2898
                     /locus_tag="VM406_03025"
     CDS             2428..>2898
                     /locus_tag="VM406_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVL74964.1"
                     /translation="MIRDPEGFASFLQELRRFVRERLVPQEKTVMEQNEVPQAIVDEM
                     AGQGLFGYSIPEEYGGAGMTTEELVLAAIELSQCSVAFRARVGTNTGIGSEGLVADGT
                     AEQKRKYLPRLASGEITGAFALTEPEAGSDATSLRSSARRDGDDYILNGVKCFIT"
BASE COUNT          509 a          926 c          974 g          489 t
ORIGIN      
        1 acccgacagc accggcaccg agatcgacct gtccgccacc gaggcgatgc tgcgcatgct
       61 cgatccgctg gcggccgaat acgagaagag cggcgtcaat cgccagcgcg ccggcaatcg
      121 cgcgacctac accgcgccgt cgaacatgta caagaccggc gatggcgcct acgtgaccat
      181 cgtcggatcg tcgtccgtga tcttccgccg cctgtgccag gcgatgggcc gcgccgaact
      241 ggccgacgat gcgcgattcg ccagcaatcg cagccggatg gagaacgtcg acgcgataga
      301 cggagagatc gcgggctggt gcggctcgcg cacctacgat gaggtcgccg tcgccctcac
      361 cgagtttgcg gtgccgttca gcaagatcta ttctgcggcc gacatcgtcg ccgatccgca
      421 tttcatcgcg cgcggtgcgg tgatccgctt gccggatgcc gagcatggct cgctgccggc
      481 gccttgcgtg gtgccgcgct ttgccggcta tcagccggaa ccgccgcgta ccggaccggc
      541 gccgggggag cacaatgagg aagtgttcgg cgcgctcggt ctatgcgcgg aaacgctcgc
      601 ccagctgcgc gcacgcgggg tggtatgaga agcatggctg cgcgatcgct gctatagtcg
      661 gccgattcaa tcgatgcagc gatggaagag attcgcaagg ggcttgcgcc cgaggaagac
      721 gacccgcagt tcgcgcgaac gctcgcgcgc ggcatcgata tcctgctttg ctatcgggtg
      781 ggcgaatcgg tgctcggcaa caaggatttc gccgcgcgca ccggactgtc caagaccacg
      841 gtcgcgcgcc tgatgcatac gctcgcgcag ctcggttact tgcgccacga tcccaaactc
      901 gcgaagtacc ggctcggcgc gccggtgctg tcgatgggct atccgctgct ggccagcatg
      961 cagatccgcc agctcgcgcg gccgctgatg cgcgaactgg ccgagcgcac cggcggcgcg
     1021 gtgtcgctcg ggatgcgcga ccgaaccagc atgatctacg tcgaaacctc tcgctcgacc
     1081 gacagactgg tcagaacccc ggacatcggc gcgccgattc ccattctgac caccgcgatg
     1141 ggccgggcct ggctgtgcaa ggcggatgcg cgcgagcgcg acgaggtgct caaccgcatc
     1201 cgtctgaacg atccggaaca tttcgaacgt cactatccgg ccgcgatgca ggcggtgcgc
     1261 gagttcggac agcgcaagta ctgtcgcaac cgcggcgaat ggcgtgggga tgtctacgga
     1321 ttcgcggttc cgcttgcgcg cagcttccag tcccagcagt tcgtcgtcaa ttgcggcatt
     1381 ccggctgccg cccatgcgtc tgctgcggcg gccgagaaag aggtggcgcc gcagctgatc
     1441 tcgctcgtgc agagcctgca gatcacgctc ggcatgacat cgtaagaaag ggattaggcg
     1501 cccgggccgc tcacagcgcc atctctatcc tgcgcgcggt ttcgatcagg cgctctccca
     1561 gcctgcgttc gatcgcctcg cccttcaggc ggcgcgtcag gacgccgcag ttcagcacca
     1621 ccacctcgtc atcgacaggc ttgcgcagcg ggatcgccag cgcgtggatg tcgggctgcc
     1681 agtcgccgtg cgatacacag aagcccttgg tctccagatc cgccacagcc gtcgcaatca
     1741 ctttcctcgc ccgggtacgc agcgagcggt tgccttgcgc gaacgcctcg atcacgtcgt
     1801 cgcgctggcc gggcggcgcc gccgccagcc aagcccgccc catcgcgctc gccaatattg
     1861 gcagcgaggc gccgatgtcc gggcggaact cgatcgattc atggccgcgg ctggtttcga
     1921 tgtagaccac gttgagacgg tcgcgcatgc ccagcgacac cgaaccttct gcaaagtcgg
     1981 ccagctgctg catcaggggg cgggcgatct ggcgcacacg caagtgggcc agcagcggat
     2041 agccgagcga cagtgcggtc gggcctagtt gatagcggcg cgtgtgcgca tcgtaggcga
     2101 gcaggccgcg actcatgagc gtgtaggtca ggcgcgtgat ggtcgacttg gagaggccgg
     2161 tggcgtccga tagttccttg ttgctcagcg tcggggcatc gacgcgaaag cattgcagca
     2221 gcgcgaggcc gtgggcgagc gtggtggcaa aactggggtc cggatgacgc atgatcagtg
     2281 gcgtgcagcg caaggggcag gggcatcata gcggacatca gatcgcatat gtccggccgt
     2341 tgttcaagat atcgaaacaa gcgtttcgct tcggtcgcga tggttctagt ctgcagtcac
     2401 cacaatgatt catgaaggag accagacgtg atccgcgatc ccgaagggtt tgcgtctttt
     2461 ctgcaggaac tgcgccgctt cgtacgcgag cgcctcgttc cgcaggaaaa gacggtgatg
     2521 gagcagaacg aggtgccgca ggccatcgtt gacgagatgg ccgggcaggg cctgttcggc
     2581 tattccattc ccgaggagta tggcggagcg ggaatgacca ccgaggagct ggtgctggcg
     2641 gccatcgaac tgtcgcagtg ctcggttgcg ttccgcgccc gtgtcggaac gaataccggc
     2701 atcggatccg aaggcctggt cgccgacggc accgcggaac agaaacgcaa gtatctgccg
     2761 cggctggcca gcggcgagat caccggcgcg ttcgcgctga ccgagcccga ggcgggctcg
     2821 gacgccacct cgctgcgttc gagcgcgcgg cgcgacggcg acgattacat cctcaacggc
     2881 gtgaagtgct tcatcacg
//
LOCUS       DATLGF010000102         8070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_51449,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000102 DATLGF010000000
VERSION     DATLGF010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 8070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8070
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51449"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1023
                     /gene="ybiB"
                     /locus_tag="VM406_03030"
     CDS             49..1023
                     /gene="ybiB"
                     /locus_tag="VM406_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HVL74965.1"
                     /translation="MTTTNEPFRAAPFIKEIGRGKKGARDMSREDARTLYTAMLERRV
                     SDLELGGILIAMRIKGESVDEIAGFLDAAEAAFERLPDPGGEYAPIIIPSYNGSRQMP
                     NLTPLLALLLARAGAPVLIHGVTKDPGRVTTAEVLRELGHEFATTHEQASAGFAAGRP
                     VFMPIESLAPRLAEMLALRRVLGLRNSTHTLVKIMQPFACPALLLTSYTHPDYLEMLG
                     AYFSTAAAVERGDAFLMRGTEGETVANAKRGQQIAWFHEHQRTVLAERQAPTDDTPPL
                     PEASDAASTARWIEAVLKGKQAVPGPIGGQIEHCLNVARGLRRRFANG"
     gene            1295..2491
                     /locus_tag="VM406_03035"
     CDS             1295..2491
                     /locus_tag="VM406_03035"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVL74966.1"
                     /translation="MTTHFDVAVIGAGAAGMMCAAVAAQRGRKVVLIDHAAKLAEKIR
                     ISGGGRCNFTNLHAGPQNYVSENPHFCKSALARYTPQDFLALVRRYRIAYHEKHKGQL
                     FCDDSAEQIIEMLKAECDVGGVAWRMPCKVEALRKEGERFMLLTSAGEYHADNVVIAT
                     GGLSIPKIGATDFAYRIASQFGLKQIAPRPGLVPLTFDAAGWQPFLPLAGIALEVDIE
                     TGQKKTRMQFREDLLFTHRGLSGPAVLQISSFWQPGAPLVVNLLPELDAEAALLEQKA
                     SSRKQLGNVLAQWLPARLAEGLLEAHGFDPATRLADMQDKKLRSLGAALNRWTIVPDG
                     SEGYRKAEVTCGGVDTRELSQQTMMANRAPGLYFIGEAVDVTGWLGGYNFQWAWASGF
                     AAGKAI"
     gene            2563..2775
                     /gene="rpsU"
                     /locus_tag="VM406_03040"
     CDS             2563..2775
                     /gene="rpsU"
                     /locus_tag="VM406_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HVL74967.1"
                     /translation="MTTIRVKENEPFEVAMRRFKRTIEKTGLLTELRAREFYEKPTAE
                     RKRKLAAAVKRHYKRIRSQQLPKKLY"
     gene            2911..3360
                     /locus_tag="VM406_03045"
     CDS             2911..3360
                     /locus_tag="VM406_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HVL74968.1"
                     /translation="MSLKEQITEDMKAAMRAKDTGRLSTIRLLTAAMKQKEVDERIEL
                     TDAHVLAIIEKMIKQRRDSISQFEAGGRQDLAEIEKAELAVLSGYMPAALSDAEVQAE
                     VDAAIAAAGASGPQDMGKVMGVLKPRLSGRADMTAVSAKVKAALAKA"
     gene            3456..5249
                     /gene="dnaG"
                     /locus_tag="VM406_03050"
     CDS             3456..5249
                     /gene="dnaG"
                     /locus_tag="VM406_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HVL74969.1"
                     /translation="MIPQSFIQDLLNRVDIVDVVGRYVQLKKGGANYMGLCPFHNEKS
                     PSFTVSPTKQFYHCFGCGAHGTAIGFLIEYSGMGFVDAVKDLAQSVGMTVPEDDRLPP
                     AQRAEVQAKSVALTEVLAKACDFYRHQLRGAPHAIAYLKGRGLTGEIAAKFGLGYAPD
                     GWDSLRAVFPDYGLDALVESGLVIDKSEEEGGGRKRYDRFRDRIMFPIRNTKGQVIGF
                     GGRVLGQGEPKYLNSPETPLFQKGYELYGLFEARQAIREAGYVLVTEGYMDVVALAQL
                     GFPQAVATLGTACTPTHVQKLLKQTDQVVFSFDGDAAGRRAARRALDACLPHATDSKT
                     IRFLFLPPEHDPDSFVREQGAEAFERALREAMPLSQFLVNEVVAGKDLDTPEGRAHAQ
                     HEAKPLLQAMPASALRWQIVRTLAQTTRTSPAELEALLELPQPVARVKAAPPRAKRSA
                     PMGLERQMIRLLLAHPGLAAMLDEAALDAAARMAPDNAALLRQLVDACRALGEGASFA
                     ALAEQLRAAGPDFDALIAEIAGEGESDQETARLELAGAMRQTRLQMVRAEMEQLAASG
                     LASEDARTRYRALMQEQEQLRRPVQVESTVR"
     gene            complement(5449..5715)
                     /locus_tag="VM406_03055"
     CDS             complement(5449..5715)
                     /locus_tag="VM406_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74970.1"
                     /translation="MAVVLVAPFGAGFADVLVGIFAATDAFAPVLVAAGFAIDLPEAA
                     LVAAAPGFFAADFAAGVAFAGFFIAFAMDSIQMIALLRREVCAW"
     gene            5840..8062
                     /gene="rpoD"
                     /locus_tag="VM406_03060"
     CDS             5840..8062
                     /gene="rpoD"
                     /locus_tag="VM406_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HVL74971.1"
                     /translation="MTNKKTEVKAAKPSKAQASTESKIESKVMAVSQTTDAAVLASID
                     TSGYVLPAVKVPGRRGRKPKDFQPENDEVAALNAVERAELKAADKARAKDRKAKEKAL
                     LKDAFSSDTEASEEEIEQRRQKLKTLIKLGKDRGFLTYSEINDHLPENIVEPEAIEGI
                     IGTFNDMGIMVYEQAPDAETLLLSDSVATAATDDDADAAAEAALSTVDSDFGRTTDPV
                     RMYMREMGSVELLTREGEIEIAKRIEEGLKDMIQAISACPTTIAEILAAADRIARDEI
                     KIDEIVDGLVDPNAVEEVAPVAVAAAAASDDEDEDEDEDEDEAEEEENTSGGAAGFSA
                     EQLEQLKRDSLALFDEIAAQFDKMRKAYEKEGYASKPYVKAQETISNTLLSIRFTAKF
                     VEKLCDTLRGQVDEVRQIERQILDVAVNRSGMPRTHFIEVFPGNETNLDWVDGEVAAG
                     KPYSVVLGRNVPAIKELQQKLIDLQARVVLPLPDLREINKKMSAGEMKARRAKREMTE
                     ANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAI
                     TRSIADQARTIRIPVHMIETINKMNRISRQILQETGAEPDPATLAIKMEMPEDKIRKI
                     MKIAKEPISMETPIGDDDDSHLGDFIEDNNTLAPADAALHASMRGVVKDVLDSLTPRE
                     AKVLRMRFGIEMSTDHTLEEVGKQFDVTRERIRQIEAKALRKLKHPSRSRKMRSFLDQ
                     "
BASE COUNT         1657 a         2606 c         2568 g         1239 t
ORIGIN      
        1 ccgccgcatt gtcacgcgca cccttccttc ggtgtaccct cccacctcat gaccaccacc
       61 aacgaaccat tccgcgccgc ccccttcatc aaggaaatcg gccgcggcaa gaagggcgcg
      121 cgcgacatga gccgtgagga cgcccgcacg ctgtacaccg cgatgctcga gcgccgcgta
      181 tccgatctcg agctgggcgg catcctgatc gccatgcgca tcaagggcga gtcggtcgac
      241 gagatcgccg gcttcctcga tgccgccgaa gccgcgttcg agcggctgcc cgatcccggt
      301 ggcgagtatg cgccgatcat cattcccagc tacaacggct cgcggcaaat gccgaacctc
      361 acgccgctgc tcgcgttgct gctcgcgcgt gccggcgccc cggtgctgat tcatggggta
      421 acgaaggatc cgggcagggt gaccaccgcg gaagtgctgc gcgaactggg gcatgagttt
      481 gcaacaacgc atgaacaggc tagcgccggc ttcgcggcgg gccggccggt cttcatgccg
      541 atcgagtcgc tggctcctcg ccttgcggag atgctggcac tgcgccgcgt gctcggcttg
      601 cgcaattcca cgcacacgct ggtgaagatc atgcaaccct ttgcctgccc ggcattgctg
      661 ctgacgtcct acacccatcc cgactacctg gagatgctcg gcgcctattt ctcgacggcc
      721 gctgcggtcg agcgcggcga cgccttcctg atgcgcggca ccgaagggga gacggttgcg
      781 aacgcgaagc gcggacagca gatcgcgtgg tttcatgaac atcaacgcac ggtgctggcc
      841 gagcgccagg cgccgaccga cgacacgcct ccgttgcccg aggccagcga tgcggcaagc
      901 accgcacgct ggatcgaagc ggtattgaag ggcaagcaag cggtgcccgg tccgatcggc
      961 gggcagatcg agcattgcct gaacgtcgca cgcggcttgc gacgtcggtt tgccaacgga
     1021 tgaggtcctt cgcctccgct caggacgaca gcatttcgtg agcgtgtcgt cctgagccgc
     1081 aggcgaagga cctcatccgc atcaagcggg catcgtcgcc tggcgcaggg atgaggtcct
     1141 tcgcattcgc tcaggacgac aacactcctt gaacctgtcg tcctgagccg caggcgaagg
     1201 acctcatccg cccgacacgt gccggcgcgt gcatcgcggc aagcgtcatc gcccccatac
     1261 cgtacaatcc gcactccact cgcatcccac gcctatgacc acccatttcg acgtcgccgt
     1321 catcggcgcc ggcgctgccg gcatgatgtg cgcagccgtc gccgcccagc gcggacgcaa
     1381 ggtcgtgctg atcgaccatg ccgcgaaact tgccgaaaaa atccgcatat ccggcggcgg
     1441 ccgctgcaat ttcaccaacc ttcatgccgg tccgcagaac tacgtgtcgg aaaatccgca
     1501 tttctgcaag agcgcgctgg cgcgctacac gccgcaggat ttcctcgcgc tggtcaggcg
     1561 ctaccgcatc gcgtaccacg agaagcacaa gggccagctg ttctgcgacg attccgccga
     1621 acaaatcatc gagatgctca aagccgagtg cgacgtcggc ggcgtggcct ggcgcatgcc
     1681 gtgcaaggtg gaggcactgc gcaaggaggg ggagcgcttc atgttgctga ccagcgcagg
     1741 cgaataccac gccgacaacg tggtgatcgc taccggcggg ctgtcgatcc cgaaaatcgg
     1801 cgccaccgac ttcgcctatc ggatcgcaag ccagttcgga ctgaaacaga tcgcgccgcg
     1861 ccccgggctg gtgccgctga ccttcgatgc cgccggctgg cagcccttcc ttccgctggc
     1921 cggtattgca ctcgaagtgg acatcgaaac cgggcagaag aaaacccgca tgcagttccg
     1981 cgaagacctg ctgttcaccc atcgcggcct gtccggtccg gcggtgttgc agatctccag
     2041 cttctggcag ccgggtgcgc cgctcgtcgt caacctactt ccggagctgg atgcggaggc
     2101 agccttgctc gaacagaaag cctcgagcag gaagcagctc ggcaacgtgc ttgcgcagtg
     2161 gctgccggca cgcctggccg agggcctgct cgaggcccac ggtttcgatc cggccacccg
     2221 cctggcggac atgcaggaca agaaactgcg ctcgcttggc gcggcgctga accgctggac
     2281 catcgttccc gacggctcgg aaggctaccg caaggccgaa gtgacctgcg gcggcgtcga
     2341 cacgcgcgaa ctgtcccagc agacgatgat ggccaaccgg gcgccgggcc tttacttcat
     2401 cggggaggct gtcgatgtga ccggctggct cggcggatac aacttccagt gggcatgggc
     2461 aagcggcttt gctgccggga aggcaatctg agcgcagcgt ttgcatgaaa tccgaaccct
     2521 gctataatcg cgctctttcc caagacaaac cggtttcagt acatgaccac tattcgcgtt
     2581 aaagagaacg agcctttcga agtcgccatg cgtcgcttca agcgcaccat cgagaaaacc
     2641 ggtctgctga ccgagctgcg tgcgcgcgag ttctacgaga agccgactgc tgagcgcaag
     2701 cgcaagctcg ccgctgccgt gaagcgccac tacaagcgca tccgcagcca gcaactgccg
     2761 aagaaactgt actgattcgc aacgccgcac gctgtgtgcg gtgcttcgca gacccgctcc
     2821 ggaacgcgcc gcagcgggtt ttcgtgtttt atggccggat ttgcagcaga tcgcgaccgg
     2881 accgggcggc cagccagcat tggagaaacc atgagcctca aagaacaaat caccgaagac
     2941 atgaaggccg caatgcgcgc caaggacacg ggccgcctgt ccacgatccg cctgctgacc
     3001 gccgcgatga agcagaagga agtcgatgag cgcatcgagc tgactgacgc ccacgtcctc
     3061 gcgatcatcg agaaaatgat caagcagcgc agggattcga tcagccagtt cgaggccggc
     3121 ggccgccagg atctcgccga gatcgagaag gccgaactgg ccgtgctgtc gggctacatg
     3181 ccggccgcgc tgtcggacgc ggaagtgcag gcggaggtcg atgcggcaat cgccgccgcc
     3241 ggcgcatcgg gcccccagga catgggcaag gtcatgggcg tgctgaaacc caggctgtcc
     3301 ggccgcgccg acatgacggc ggtgtcggcc aaggtcaagg cggcacttgc caaggcctga
     3361 gggcatagca gaatgactgg gtgggcggac tacggccatc cgcggcgcag cgtgctgcgc
     3421 caaccccttc gactcaacgg actcagcacc tcgccgtgat tccgcaatcc ttcatccagg
     3481 acctgctcaa tcgcgtcgac atcgtcgatg tggtgggccg ctacgtccag ctgaagaagg
     3541 gcggggccaa ctacatgggc ctgtgcccgt tccacaacga aaaatccccc agctttacag
     3601 tcagtcccac caagcagttc tatcactgct tcggctgcgg cgcccacggc acggcgatcg
     3661 gcttcctgat cgagtattcc ggcatgggct tcgtcgacgc ggtgaaagac ctcgcccaga
     3721 gcgtaggcat gacggtgccc gaggacgacc ggctcccgcc ggcccagcgc gccgaagtgc
     3781 aggccaagag cgtcgcactg accgaggtat tggccaaggc ctgcgatttc taccgccacc
     3841 agctgcgcgg cgccccgcat gcgatcgcct acctgaaagg gcgcggactg accggcgaaa
     3901 tcgccgcgaa gttcggcctc ggctatgcgc cggacggctg ggacagcctg cgcgccgtgt
     3961 ttccggacta cggcctcgac gcgctggtcg aatccggtct cgtgatcgac aagagcgagg
     4021 aagagggggg cggccgcaag cgctacgacc gttttcgcga ccgcatcatg ttcccgatca
     4081 gaaacaccaa gggacaggtg atcggcttcg gcggacgcgt gctcggccag ggcgaaccga
     4141 agtacttgaa ctccccggaa acacccttat ttcagaaggg ctacgagttg tacggactgt
     4201 tcgaggcgcg ccaggccatt cgtgaggctg gttacgtgct ggtgacggaa ggttacatgg
     4261 acgtggtcgc actggcccag ctggggtttc cgcaggcggt cgcgacgctg ggaaccgcat
     4321 gcacgccgac ccatgtgcag aagctgctga agcagaccga tcaggtggtg ttcagcttcg
     4381 acggcgatgc cgcgggacgc cgcgcagcac gccgggcgct ggatgcctgc ctgccccatg
     4441 caacagacag caagaccatt cgtttcctgt tcctgcctcc cgaacatgat ccggacagtt
     4501 tcgtgcggga acagggcgct gaagcgttcg agcgcgcgct gcgtgaagcg atgccgctgt
     4561 cgcaattcct cgtcaacgaa gtggtcgcgg gcaaggacct ggatacgccg gaagggcgcg
     4621 cccatgcgca gcacgaggcc aagccgctgc tgcaggcaat gccggcctcg gcgctgcgct
     4681 ggcaaatcgt gcgcacgctg gcccagacca cgcgcacctc gccggccgag ctggaggcct
     4741 tgctggaact gccgcaaccg gtggcacggg tgaaggcggc gccgccgcgc gccaagcgca
     4801 gcgcgccgat gggcctggag cgacagatga tccggctgct gctcgcccat ccgggtctgg
     4861 cggcgatgct ggatgaggcc gcccttgatg cggcggcacg catggcaccg gacaatgccg
     4921 cgctgctgcg gcaactggtc gatgcctgca gggccttggg cgagggtgca agttttgccg
     4981 cgctggccga gcaattgcgc gccgccggac cggatttcga cgcgctgatc gcggaaatcg
     5041 cgggcgaagg cgagtcggac caggaaaccg cccgcctgga gcttgctggt gcaatgcgac
     5101 agactagact gcagatggtg cgggccgaaa tggagcagtt ggccgcgtcg gggctcgcat
     5161 ccgaggatgc gcgtacacga tatcgcgccc tgatgcaaga acaagagcaa ctccggcgcc
     5221 cggtgcaggt cgaaagcact gtgcgctagc gcattgccgt tgaaagcgga tggccgcatg
     5281 ttgtcaatct gccccggcca cggggctgcg gcgaggaaat gttgctctca ctgtgttatc
     5341 atttaaagct tttgattcaa tgtcttagag agtttctgcc ggtcgccgag ccggggaaat
     5401 ctcgaagcaa tgtcgcgcgg gctatgccga accagatctg aggcggctct accatgcgca
     5461 aacttctcgg cgcaagagag cgatcatttg gattgaatcc atggcaaatg caatgaaaaa
     5521 acccgcaaaa gcgacgccgg cagcgaaatc cgccgcaaaa aaaccgggcg ctgctgcaac
     5581 taaagccgcc tccggcaagt cgatggcaaa gcctgccgcg actaagaccg gcgcgaaagc
     5641 gtcggttgcg gcgaagattc cgaccaagac atccgcgaag cccgctccga aaggggcaac
     5701 caggacgact gccaagtctc agggtgtgaa gtccgcggtg accaagtcgg ctcaggcgac
     5761 cgcacccagg gcggtcgcac cgaaagcgaa aacttcatcc cgagcagcag tagcggctca
     5821 agagaaattg aaagtacccg tgacgaacaa aaaaaccgaa gtaaaggctg ccaagccctc
     5881 caaggcgcag gcaagcacag agtccaagat cgaatcgaag gtgatggcgg tgagccagac
     5941 caccgacgcg gccgtgctgg cctcgatcga tacgtcgggc tacgtgctgc cggcggtgaa
     6001 ggtgcccggc cgccgcggcc gcaagccgaa ggatttccag cccgagaacg acgaggtcgc
     6061 cgcgctgaac gcggtcgagc gcgccgagct caaggccgcc gacaaggcgc gcgcgaagga
     6121 tcgcaaggcc aaggaaaagg ccttgctgaa ggacgcattc tcgtctgaca ccgaagccag
     6181 cgaagaggaa atcgagcagc gccgtcagaa gctcaagacc ctgatcaagc tcggcaagga
     6241 ccgcggcttc ctgacctatt ccgagatcaa cgaccacctg cccgagaaca tcgtcgagcc
     6301 ggaggccatt gaaggcatca tcggcacgtt caacgacatg ggcatcatgg tctacgaaca
     6361 ggccccggat gccgagaccc tgctcctgtc cgacagcgtg gccaccgccg ccaccgatga
     6421 cgacgccgac gccgccgccg aagcggccct gtcgaccgtc gattccgatt tcggccgcac
     6481 caccgacccg gtgcgcatgt acatgcgcga gatgggctcg gtcgaactgc tgacccgcga
     6541 gggcgaaatc gaaatcgcca agcgcatcga ggaaggcctg aaggacatga tccaggcgat
     6601 ctccgcctgc ccgaccacga tcgccgagat cctcgccgcc gccgaccgca tcgcgcgcga
     6661 cgaaatcaag atcgacgaga tcgtcgacgg cctggtcgat ccgaatgccg tcgaggaagt
     6721 ggcgccggtg gccgtggccg ccgcagcagc gtccgacgac gaggatgaag acgaggacga
     6781 agacgaggac gaagccgagg aagaggaaaa cacgagcggc ggcgccgccg gtttctctgc
     6841 cgagcagctc gagcagttga agcgcgactc gctcgccctg ttcgacgaga tcgccgccca
     6901 gttcgacaag atgcgcaagg cctacgagaa ggaaggctac gcgtccaagc cctacgtgaa
     6961 ggcgcaggag accatctcga acaccctgct gtcgatccgc ttcaccgcaa agttcgtcga
     7021 gaagctgtgc gacacgctgc gcggccaggt cgatgaagtg cgccagatcg agcgccagat
     7081 cctcgacgtc gccgtgaacc gcagcggcat gccgcgcacg cacttcatcg aggtcttccc
     7141 gggcaacgag accaacctcg actgggtcga cggcgaagtc gcggccggca agccgtacag
     7201 cgtcgtcctc ggccgcaatg tcccggccat caaggaactg cagcagaagc tgatcgacct
     7261 gcaggcgcgt gtcgtgctgc cgctgccgga tcttcgcgag atcaacaaga agatgtcggc
     7321 cggcgagatg aaggcgcgcc gcgccaagcg cgaaatgacc gaggccaacc tgcgcctggt
     7381 gatctcgatc gcgaagaagt acacgaaccg gggcctgcag ttcctcgacc tgatccagga
     7441 aggcaacatc ggcttgatga aggcggtgga caagttcgaa taccgtcgcg gctacaaatt
     7501 ctcgacgtat gcgacctggt ggatccgcca ggcgatcacc cgctcgatcg ccgaccaggc
     7561 gcgcacgatc cgcatcccgg tgcacatgat cgaaacgatc aacaagatga accggatctc
     7621 gcgccagatc ctgcaggaaa ccggcgccga gccggatccg gcgacgctgg cgatcaagat
     7681 ggagatgccg gaagacaaga tccgcaagat catgaagatc gcgaaggaac cgatctccat
     7741 ggagacgccg atcggcgacg acgacgactc gcatctgggc gacttcatcg aggacaacaa
     7801 tacgttggcc ccggccgacg cggcgttgca tgcctcgatg cgcggcgtgg tcaaggacgt
     7861 gctcgattcg ctgacgccgc gcgaggccaa ggtgctgcgc atgcgcttcg gcatcgaaat
     7921 gtcgaccgac cacacgctgg aagaagtcgg caagcagttc gatgtcaccc gcgagcgcat
     7981 ccgccagatc gaagccaagg cgctgcgcaa gctcaagcat ccgagccgct cgcggaagat
     8041 gagaagcttc ctcgaccaat agggtggccc
//
LOCUS       DATLGF010000103         5173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_36730,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000103 DATLGF010000000
VERSION     DATLGF010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36730"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..2106)
                     /gene="uvrB"
                     /locus_tag="VM406_03065"
     CDS             complement(13..2106)
                     /gene="uvrB"
                     /locus_tag="VM406_03065"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HVL74972.1"
                     /translation="MADLSQASSSVDESRIITFPDSPYRLYQPFPPAGDQPDAIAKLV
                     EGIEDGLSFQTLLGVTGSGKTYTMANVIARAGRPAIIFAPNKTLAAQLYSEFREFFPH
                     NAVEYFVSYYDYYQPEAYVPQRDLFIEKDSSINEHIEQMRLSCTKSLMERRDVVIVAT
                     VSAIYGIGNPNEYHRMILTLRAKDKVSQRDVIARLIQMQYTRNEVDFGRGTFRVRGDT
                     IDVFPAEHAELALRIETFDDEIDSLQLFDPLTGRVRQKIPRFTIYPGSHYVTPRETVL
                     RAIESIKEELRERLEFFRREGKLVEEQRLEQRTRFDLEMMQEIGFTKGIENYSRHLSG
                     AKPGEPPPTLVDYLPQDALMFLDESHVLLGQLNGMYNGDRARKVNLVDYGFRLPSALD
                     NRPLKFQEFEAKMRQTVFVSATPAEYENSHAGQVVEQVVRPTGLVDPAIIVRPASTQV
                     DDLMNEITERVKKDERVLVTTLTKRMAEQLTEFLSDNGVKVRYLHSDIDTVERVEIIR
                     DLRLGTFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRSERSLIQTIGRAARNLNG
                     TAILYADQMTDSMRRAIGETERRRAKQIAFNAAHGITPAGIKKEIRELIDGVYNPQEA
                     RLEQQVAQDQARYEGMSEKQVSKEIKRLEKLMVEHAKNLEFEKAAQVRDQLHVLKEQL
                     FGAPGADNVVPLAGK"
     gene            2257..3471
                     /locus_tag="VM406_03070"
     CDS             2257..3471
                     /locus_tag="VM406_03070"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HVL74973.1"
                     /translation="MTQAHSASLFAAVEMAPRDPILGITEAFNADKNPNKVNLGVGVY
                     YDDNGKVPLLQCVRKAEAQLAELAAPRTYLPIEGLAAYDRAVQDLVFGADSTVVQEKR
                     VITAQALGGTGALKLGADFLKRFAPNAEVWISDPSWENHRALFESAGFKVNNYPYYDP
                     ATRGVNFEGMLGALKTMPAGAIVVLHACCHNPTGADLSDAQWQDVIKAVTERGLVPFL
                     DMAYQGFGDGIAEDGKVVRQFAETGAPLFVSNSFSKSFSLYGERVGALSIVSSSSEES
                     ARVLSQLKRVIRTNYSNPPVHGGQVVATVLSSPELRALWEEELAAMRVRIREMRQALV
                     AKLKEKAPGKDFDFVIRQRGMFSYSGLSKEQVGRLRDEFSVYAVDTGRICVAALNSRN
                     IDAVTNAIAKVL"
     gene            3556..3631
                     /locus_tag="VM406_03075"
     tRNA            3556..3631
                     /locus_tag="VM406_03075"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3589..3591,aa:Asn,seq:gtt)
     gene            4106..4861
                     /locus_tag="VM406_03080"
     CDS             4106..4861
                     /locus_tag="VM406_03080"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HVL74974.1"
                     /translation="MSRAFLESTTSVTSSKGQPAIELIVRAESHSALRVAVEHGADTI
                     RLACRTRSSVGAATRHGLHRAGMKKVVQLARRHGCAVGLDLDAPDLEQSAVGALLHSL
                     DYGIDSVCVTSPALALYLRMNHPAIRVSLQVSDAALDKRALTLLRLRLGIERIVLPRV
                     VSLVQLRQAAQVHGLHLELFGYGAGCALVAGRRESAGASVVAAAHTATEGLLGRCSTG
                     LVASNDSGFGISHEDDGMVRRAMPSSCGTAFVA"
     gene            complement(4957..>5173)
                     /locus_tag="VM406_03085"
     CDS             complement(4957..>5173)
                     /locus_tag="VM406_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HVL74975.1"
                     /translation="STACGLLVCVVIASLFIGTAIQVELATFIAVCFVAGMFSLIGSF
                     IYLLREVFIATRVLSAQRLIVMDKASS"
BASE COUNT          936 a         1638 c         1563 g         1036 t
ORIGIN      
        1 attgtacgat gcctactttc cggccagcgg caccacgttg tccgcgccgg gcgcgccgaa
       61 cagctgctcc ttcagcacgt gcagctggtc gcgtacctgc gcggctttct cgaactcgag
      121 attcttcgcg tgctcgacca tcagcttttc gagccgcttg atctccttgc tgacctgctt
      181 ctcgctcatc ccctcgtagc gcgcctgatc ctgggcgacc tgctgctcca gccgcgcctc
      241 ctgcgggttg taaacgccgt caatcagctc ccggatctcc ttcttgattc cggccggcgt
      301 gatgccatgt gccgcgttga aagcgatctg cttggcgcgg cggcgctcgg tttcgccgat
      361 cgcccggcgc atcgaatcgg tcatttggtc cgcataaaga attgcagtgc cgttcaggtt
      421 gcgtgccgcg cggccgatcg tctggatcag gctgcgctcg gagcgcagga agccctcctt
      481 gtccgcatcg aggatcgcaa ccagcgacac ttccggaatg tcgagccctt cgcgcagcag
      541 gttgatcccg accagcacat cgaaggtgcc caggcgcagg tcgcggatga tctccacccg
      601 ctcgacggta tcgatgtcgc tgtgcaggta gcgcaccttc acgccgttgt cgctcaggaa
      661 ctcggtcagc tgctcggcca tgcgcttggt caacgtggtg accagcacac gctcatcttt
      721 cttcacgcgc tcggtgatct cattcatcag atcgtccacc tgggtgctgg ccggacgcac
      781 gatgatcgca ggatcaacca ggccggtggg ccgcaccacc tgctcgacca cctgccccgc
      841 atggctgttc tcgtactctg ctggggtcgc ggacacgaag accgtctgcc gcatcttggc
      901 ttcgaattcc tggaacttga gcggccggtt gtccagcgcc gaaggcaggc ggaacccgta
      961 gtcgaccagg ttcaccttgc gcgcgcggtc gccgttgtac atgccgttca actggccgag
     1021 caacacatgc gactcgtcga ggaacatcag cgcgtcctgc ggcagatagt cgaccagcgt
     1081 gggcggaggt tcgcccggct tggcgccgga gaggtggcgc gaatagttct cgatgccctt
     1141 cgtgaagccg atctcctgca tcatttcaag gtcgaaacgg gtacgctgct ccaggcgctg
     1201 ctcttcgaca agcttgcctt cccgccggaa gaactccagc cgctcgcgca gctcctcctt
     1261 gatgctttcg atcgcgcgca gcacagtctc gcgcggcgtg acgtaatgcg aaccgggata
     1321 gatcgtgaaa cgcgggatct tctggcgcac ccgtccggtc agcggatcga acagctgcag
     1381 gctgtcgatc tcgtcgtcga aggtttcgat gcgcagcgcc agctccgcat gctccgcggg
     1441 gaacacatcg atcgtgtcgc cgcgcacgcg gaacgtgccg cgaccgaaat cgacctcgtt
     1501 gcgcgtgtac tgcatctgga tcaggcgcgc gatcacgtcg cgctggctga ccttgtcctt
     1561 cgcgcgcagc gtcaggatca tccggtggta ttcgttcgga ttcccgatac cgtagatcgc
     1621 cgagaccgtc gccacgatca ccacgtcgcg ccgctccatc agggacttgg tgcaggaaag
     1681 ccgcatctgc tcgatatgct cgttgatcga ggaatccttc tcgatgaaca ggtcgcgctg
     1741 cgggacgtag gcttccggct ggtagtaatc gtagtagctg acgaagtatt cgaccgcgtt
     1801 gtgcggaaag aactcgcgga actcgctgta caactgcgcc gccagcgtct tgttcggcgc
     1861 aaaaatgatg gccggccgcc ctgccctggc gatcacgttc gccatcgtgt atgtcttgcc
     1921 ggaaccggtc acgccgagca gcgtctggaa cgacaatccg tcttcgatgc cttccaccag
     1981 tttggcaatg gcatcgggct ggtcgccggc cggagggaac ggttgataca accggtaggg
     2041 cgagtcgggg aaggtgatga tccgcgactc gtcgacgctg ctgcttgcct gggataaatc
     2101 tgccatctgg ataggacctt tggtaaaatt caaggtttcg cgccgggcgg ttcccctccc
     2161 gcaccaaccc gccgacaatg cgtcagccag cgcccgaata agcgcatgta taagcgtccg
     2221 tagcctcttc cgcagtcaac ttttgatggt aacacgatga cccaagccca ctccgcctcc
     2281 ctttttgcgg ccgtcgaaat ggcgccgcgc gatccgattc tcggcatcac cgaagccttc
     2341 aacgcagaca agaacccgaa caaagtgaac ctcggcgtgg gcgtctacta cgatgacaac
     2401 ggcaaggtgc cgctgctgca atgcgtgcgc aaggccgaag cgcagctggc cgaactggcc
     2461 gcgccgcgca cctacctccc gatcgaaggc ctcgcagcat atgaccgcgc ggtacaggat
     2521 ctggttttcg gcgccgactc cactgtcgtg caggaaaagc gtgtcattac ggcacaggca
     2581 ctgggcggca ccggtgcact gaagcttggc gccgacttcc tgaagcgctt cgcgcccaac
     2641 gcggaagtgt ggatcagcga tcccagctgg gagaatcacc gcgcgctgtt cgagagcgcc
     2701 ggcttcaagg tgaacaacta tccgtactat gaccctgcca cccgtggcgt caacttcgag
     2761 ggcatgctgg gcgccctgaa gaccatgcct gcgggcgcca tcgtcgtgct gcacgcgtgc
     2821 tgccacaatc cgaccggcgc cgacctgtcc gatgcacaat ggcaggacgt catcaaggcg
     2881 gtgaccgaac gcggcctcgt tcccttcctc gacatggcct accagggctt cggcgacggc
     2941 atcgccgaag atggcaaggt ggtgcgccaa tttgccgaaa ccggcgctcc gctgttcgtc
     3001 tccaactcgt tctccaagtc cttctcgctg tacggtgagc gcgtcggcgc gctcagcatc
     3061 gtgtcttcca gcagcgaaga atcggcacgc gtgctgtcgc aactgaagcg cgtgatccgc
     3121 accaactact cgaatccgcc cgtccatggc ggccaggtgg tcgcaaccgt gctttcctcg
     3181 ccggagcttc gcgcgttgtg ggaagaggaa cttgctgcca tgcgcgtgcg catccgcgaa
     3241 atgcgccagg cgctcgtcgc caagctcaag gaaaaggcgc cgggcaagga tttcgatttc
     3301 gtgatcaggc agcgcgggat gttctcctac tcgggtctga gcaaggagca ggtcggtcgg
     3361 ctgcgcgatg aattctcggt ttatgcagtc gacaccggcc gcatttgcgt ggccgcgctg
     3421 aactctcgca acatcgacgc agtcacaaac gcgattgcca aggtgctctg atttttgagc
     3481 gggccgccgg gcaaaaaatt tcccggcggc tttgacacac tgagaaaaat cggcctataa
     3541 tagcgtctct gctgttccct gatagctcag tcggtagagc gacggactgt taatccgcag
     3601 gtccctggtt cgagtccagg tcggggagcc aaaaaatcct aaaagcccag cctcagcgct
     3661 gggctttttc atttctcccg cgcattcgcc cccgagcacg attccttccc ccgtttgatt
     3721 cccggcaaag tgttgtctca ctggcgcgct atgttgtggc gataggcatt ttcgcctccc
     3781 acaatcctcc aggagctcaa aaatgtatat cggactggga acactgctcg tcatcatcat
     3841 cctgattctc ctcttcacct gaccctccct gccatgcggc gcttcggcgc cgattcttgc
     3901 cttccggtac gtctcttgag ataagtcaaa tccgccggca atcgtgttgc taacgtgaaa
     3961 tcagtgcagg atcgctggtt gaggttgcgc ggccggcatc ccgccgcatg aagacttctt
     4021 ttgaatgaac accgggcctc ggcgcagcca ggcaggatcc cagaacgcgc cggtcgtccg
     4081 tgtatgcgga gaaaggagac ggagcatgag ccgagctttc ctggaatcga caaccagcgt
     4141 aacatcgtca aaaggtcagc ctgcgatcga actgatcgtc agggcggaga gccactccgc
     4201 tcttcgggtg gcggtagaac atggggcgga cacgatcagg ctggcctgcc gcacccgatc
     4261 cagcgtcggc gccgctactc gacacggcct gcatcgcgcg ggaatgaaga aggtggttca
     4321 actggcgcgt cgccatggct gcgcggtcgg gctcgatctt gatgcgcctg atctggaaca
     4381 atcggctgtt ggtgctctgc tgcacagtct cgactacggg atcgactcgg tctgcgtgac
     4441 ttcccccgcc ctggcgctgt atctgcgcat gaaccatccc gcgatccggg tctccttgca
     4501 ggtaagtgac gctgcgctgg ataaacgtgc gctgacgctg ctgcggctgc gccttggcat
     4561 cgaacgcatc gtcctgccgc gggtggtcag tttggtgcaa ctacgccagg cggcacaggt
     4621 gcacggcctg catctcgagt tgtttggcta cggggccggc tgcgcactgg ttgccggacg
     4681 ccgggagagt gccggggcca gcgtcgtggc ggccgcgcac actgctacgg aaggcctgct
     4741 tggccgctgc tccaccggct tggtggcgag caacgacagc ggttttggca tatcgcatga
     4801 ggatgacgga atggtgcggc gcgcgatgcc cagctcgtgc gggacggcgt tcgtggcgtg
     4861 attctggacg caccccgttc cactacaacc gtcaccggcc tggtgcgcgc atggcgcacg
     4921 gcactggatt gctctcctgc agaagcgcgg gcgtcgctat gacgacgctt tgtccatcac
     4981 tatcaggcgc tgggccgaca gcacacgggt cgcgatgaag acctcacgaa gcaggtagat
     5041 gaagctgccg atcagtgaaa acattcctgc gacgaagcag acggcgatga aggtggcaag
     5101 ctcgacctga atcgctgttc cgatgaagag cgatgcaatc accacgcaga ccaggaggcc
     5161 gcacgcggtg ctc
//
LOCUS       DATLGF010000104         3135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_36817,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000104 DATLGF010000000
VERSION     DATLGF010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36817"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VM406_03090"
     CDS             <1..755
                     /locus_tag="VM406_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HVL74976.1"
                     /translation="IILVTGPTGSGKTTTLYTTLKALATSEVNVCTVEDPIEMVEASF
                     NQMQVQHGIDLSFADGVRALMRQDPDIIMVGEIRDLETAEMAIQAALTGHLVLSTLHT
                     NDAPSAVMRLLELGVPYYLLEATLIGIMAQRLVRTLCPECKSGDGTLNDEVWAALTEG
                     WKLEKPRTVYRAVGCPACRQTGYRGRTGLYELVSVSHPFSKLIKQETDIHALRQQSIA
                     DGMKPLRIAGALKIAEGATTVDEVLKVTSSGA"
     gene            826..901
                     /locus_tag="VM406_03095"
     tRNA            826..901
                     /locus_tag="VM406_03095"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:859..861,aa:Lys,seq:ctt)
     gene            1231..1383
                     /locus_tag="VM406_03100"
     CDS             1231..1383
                     /locus_tag="VM406_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74977.1"
                     /translation="MTQFDLASLAGCGNRFLVELEKGKQTVQMQKVLDVLALLGLEVV
                     IRKKGS"
     gene            1383..2738
                     /locus_tag="VM406_03105"
     CDS             1383..2738
                     /locus_tag="VM406_03105"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HVL74978.1"
                     /translation="MAAAALPTALSVYWSDEHVGTLHRTDPLSFAYARAWLDKAAAPL
                     HPHIPLAEGTIATPAVHAFFENLLPEADQRRIIGLKHQVSSVFGLLALMGGDTAGAIV
                     LTPEGERPTPPAYQRLTWDQVNALLHADGKFVRERAAIEAEAAAAGMPVPRLSLSGAQ
                     LKMLLSIDENGNPWRPMGTSPSTHILKPDIVRTDLKLFASAVNETIVMRAARICELPT
                     ARVAYQATTKACLIERYDRYWRDDRALGRLWQADFCQIAGKASDEKYEADGGPTLKEC
                     YSIIRSYSVQPAVDLRNFLRWIFFNLYVGNNDCHAKNLSMLAGPEGLRLAPFYDLMST
                     RVYPGLAANFAFRIGGESEPGKLASPHLASLADELGLPLKYLTRIATTMASQVKSALP
                     QAVEWVKKDLGPNEAVMAARLQHKIASVTKRMQARLCGEAPHPEPKDDLEDAPAQGPH
                     A"
     gene            complement(2752..>3135)
                     /locus_tag="VM406_03110"
     CDS             complement(2752..>3135)
                     /locus_tag="VM406_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013954286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HVL74979.1"
                     /translation="VECILGAHRDPTFGPVVMFGMGGVNVELLKDVSFRLAPIGIDEA
                     HAMIAECRSAPLLDGYRGAPLADKEALAAAIVRLSELAATCGDSLESIDINPFAVLPQ
                     GRGALALDAVVIGRGAEAADPARLA"
BASE COUNT          626 a         1001 c          936 g          572 t
ORIGIN      
        1 gcatcatcct cgtgaccggc cccaccggct ccggcaagac caccacgctc tacaccacgc
       61 tgaaggcgct ggcgacttcc gaagtcaacg tctgcacggt ggaagacccg atcgaaatgg
      121 tcgaggcctc gttcaaccag atgcaggtcc agcacggcat cgatctgtcg ttcgccgacg
      181 gcgtgcgcgc actgatgcgc caggacccgg acatcatcat ggtcggcgag atccgcgacc
      241 tggaaaccgc cgagatggcg atccaggccg cgctgaccgg ccacctcgtg ctctccacgc
      301 tgcacaccaa cgacgcgccg tcggccgtga tgcgcctgct cgaactcggc gtgccctact
      361 acctgctgga agccacgctg atcggcatca tggcgcagcg cctggtgcgc acgctgtgcc
      421 cggaatgcaa gagcggcgac ggcacgctca acgacgaggt ctgggcggcg ctgaccgagg
      481 gctggaaact ggaaaaacca cgaacggtct accgcgccgt cggctgtccc gcttgccgcc
      541 agaccggcta tcgcggccgc accggcctgt acgaactcgt ctcggtcagc catccattct
      601 cgaagctgat caagcaggaa accgacatcc acgcgctgcg ccagcaaagc atcgccgacg
      661 gaatgaagcc gctgcgcatc gccggcgcgc tgaagattgc cgaaggggca acgacagtcg
      721 acgaagtgct gaaggtcacc tcgtccggtg cgtgatgcgc ggaaactttt ttcgacgaag
      781 ggctgacaag ccgcgttctt tgttgctata atgcgcgtct tcgttgggtc gttagctcag
      841 ttggtagagc agcggactct taatccgtag gtcgtaggtt cgacccctac acgacccacc
      901 agcgaattca agcaaaagcc cagtctccgg actgggcttt ttgcgtttcg gggaccccgg
      961 aaattcatga tcagtgccca cgaacgatat tttcccgatc ggtcatttct caaccattcc
     1021 cgtgcgccct ttccgcgtaa tttcccgatc ggtcattttt ttaccaaacc tcccggtaac
     1081 cgattataat tttcccgatc ggtcatttct taggtgggtt gcggcatgaa gaagattttg
     1141 tcggccggga cggaagatgc actgccttcg gccgtagcaa ccacggagca ggtcggaaac
     1201 cttgtccagc gggtccggaa ggagcagcag atgacgcaat tcgatctggc atccctggcc
     1261 ggctgcggca accgcttcct ggtcgagttg gagaagggca aacagaccgt ccagatgcag
     1321 aaggtcctgg atgtgctcgc actcctcggg ctggaggtcg tcatcaggaa gaagggctcg
     1381 taatggcggc cgcggcgctt cccactgccc tctcggtcta ttggagcgat gaacatgtcg
     1441 gcacgctgca tcggaccgac ccgctctcat tcgcctatgc acgggcttgg ctggacaagg
     1501 ccgcagcgcc cttgcacccg cacatcccgc tcgcggaagg caccatcgca acgcccgcgg
     1561 tgcatgcgtt cttcgagaac ctgcttccgg aggccgacca gcgcaggatc atcggcctga
     1621 agcaccaggt ctcgtcggta ttcggactac tggctctgat gggcggcgat actgccggcg
     1681 ccattgtgct cacgccggaa ggcgagcgcc ctacccctcc tgcctatcag cgtttgactt
     1741 gggatcaagt aaacgctctt cttcatgctg acggtaagtt tgtccgggaa cgtgcggcga
     1801 tcgaagcaga agccgctgct gcgggaatgc cggtcccgcg gctttcgcta tccggggcac
     1861 agctgaaaat gctgctttcg atcgatgaga acggcaatcc ttggcggccc atggggacct
     1921 cgccgtcgac ccacatactg aagccggaca tcgtccgcac agacctgaag ctctttgcat
     1981 cggccgtcaa cgagaccatc gtgatgcgtg cagcccggat ctgcgaactg ccgactgccc
     2041 gcgttgcata ccaggccacc accaaggctt gcctgatcga acgatacgat cgttattggc
     2101 gcgacgatcg ggccctgggg cgactttggc aagcggattt ctgtcagatc gcgggcaagg
     2161 catccgacga aaagtacgaa gccgacggtg gcccgacact caaggagtgt tactcgatca
     2221 tcaggtccta ctcggtgcag ccggccgtcg atttgcgcaa cttcctgcgc tggatcttct
     2281 tcaaccttta cgtcggcaat aacgattgcc acgcaaagaa tctctccatg ctcgcaggac
     2341 ctgaaggact gcggctcgcg cccttctacg acctgatgag cacccgcgtg tatccggggc
     2401 tggcagcgaa cttcgctttt cgaatcggcg gcgagagcga accaggaaaa ctcgcttcac
     2461 ctcacctggc atcgctcgcg gacgaacttg gtctgccgct caagtacctg acgaggatcg
     2521 cgacgacgat ggcatcacaa gtcaaaagcg cgcttccgca agcggtcgag tgggtgaaaa
     2581 aggacctcgg tccgaacgag gcggtgatgg ccgcgcgcct gcagcacaag attgcgtccg
     2641 tcacaaaacg gatgcaggcg aggttatgcg gtgaggcgcc tcatccggag ccgaaagacg
     2701 acctggagga cgcgcccgca caagggccac acgcgtagct gctcgcagag ttcaagccag
     2761 acgcgcagga tcagcagcct cggcaccccg accaatcacc accgcatcca gcgccagcgc
     2821 cccgcgccct tgcggcagca cggcgaacgg gttgatgtcg atcgattcca gtgaatcgcc
     2881 gcatgtcgcc gcgagttccg acaggcgcac gatggctgcg gccagggctt ccttgtcggc
     2941 aagcggcgcg ccgcggtagc cgtccagcag cggggccgat ctgcactcgg cgatcattgc
     3001 atgcgcttca tcgatcccga ttggcgcgag gcgaaagctg acgtccttca gcaactcgac
     3061 gttgacgccg cccatgccga acatcacgac cgggccgaag gtgggatcgc ggtgggcgcc
     3121 gagtatgcat tcgac
//
LOCUS       DATLGF010000105         8121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_21162,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000105 DATLGF010000000
VERSION     DATLGF010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 8121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8121
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21162"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1361)
                     /locus_tag="VM406_03115"
     CDS             complement(57..1361)
                     /locus_tag="VM406_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL74980.1"
                     /translation="MIHPASASVLRRSTDDAGPGMDSARGARNEAGGAGRPDLTRRTV
                     VRALVAAAAGAALPLPLAAASPVLLGLDAEFGLAHSTSAQAIELGMRVAIDEINRAGG
                     VLGGRPLQLVTRDHRSIPARGVRNLEQLAALPDLVAVFGGRFSPVVIEQLPLIAKLRL
                     PFMAVWSSADMITDNGMQPNYVFRLSLRDSIAMPKLLSSASARGLAPVGMLLTNTSWG
                     RSNLAAAEQHVRQGGKPAIAAVAWFNAADRSFIEKYLGLVDSGARAVILVGNDEEGAT
                     LVREVAGLPRQRRVPIISHWGITGGEFFTQAGAALREVDLSVIQTFSFFRADPGAVQR
                     FLRTAAQVSEGRVRRIENIDAPVGAAHAYDATHIVARAVEQAGSTRREAVRDALERLG
                     SYDGLIKRYSPPFTPTRHDALGPAELLMTRYREDGILVPVEQ"
     gene            1491..2057
                     /locus_tag="VM406_03120"
     CDS             1491..2057
                     /locus_tag="VM406_03120"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HVL74981.1"
                     /translation="MTILVLGLVLFLGIHLVPTSPALRMQLAGRLGEHGYRGGFSAMA
                     AIGLILIVVGYGIAPPGPQLFEPLHAARVWAPAAMVLSFILLAAANMRTHLRRVLRHP
                     MLIGVGLWALVHLLANGSLRATILFGAFLAYVVLDFASATARHAVKTFSPAFSQDVIA
                     VAAGTVLALLVMALHGWLFGVAVMPFPG"
     gene            2161..3081
                     /locus_tag="VM406_03125"
     CDS             2161..3081
                     /locus_tag="VM406_03125"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF009604.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase"
                     /protein_id="HVL74982.1"
                     /translation="MRRRALLIINRNSRSGAEDAERIRARLAGCGIEVFEPKPTLHAE
                     ICDVIRNHRQKVDMIVVGGGDGSLNAVAPALVECGLPLGVIPLGTANDLARTLSIPPD
                     PDQACMIIGAGCRHRIDLGRVNGRYFFNVAHIGLGARVTHTVSGPAKRQLGAFAYFAA
                     LARALRSTRTFHALIDCDGRRLEMDAVQIAVGNGRYFGGGTTVAAAAAIDDGRFFLCG
                     VEPLGWREVIRMALRARALYAGRFEERDPVQVEQGREIFVHTRRPMIISADGELVGRT
                     PARFELLERAVEVFVPQAYLENEVKHVAQG"
     gene            3065..3502
                     /locus_tag="VM406_03130"
     CDS             3065..3502
                     /locus_tag="VM406_03130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HVL74983.1"
                     /translation="MLLRDERQAALNRVETLCINSADDYASAATQTDDAALAALFEQL
                     GQERRGMAEALAPHIRALGDLPQDPDPDREALDHVVVGLKALLSGDGGATLVEHRLRA
                     EDALREAVLAAQDADLPEHTHAALAGMLAGIDRARARLLEIRA"
     gene            complement(3518..4654)
                     /locus_tag="VM406_03135"
     CDS             complement(3518..4654)
                     /locus_tag="VM406_03135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL74984.1"
                     /translation="MITTETAAGNIAALDRSNARTAASGALTAECFEHAVPPFVEEAL
                     EGLYGNLFSTLAHQRVYGKSRSLSTFVLRDGTQLRTVWLYERRGRTVRVLNEGIEVHA
                     HEVARFAELVFAPGSAAGAITFHAIQPRVQGLALPWRRYNCLEDMVLELPPTSTEYLA
                     QLGKSTRSYIHRYMNKLRRDFPSVRFELVEAHEIDEGKIRRIIELNRARMSHKGKVSI
                     NDDTLAEQITHLAMDCGMIGALTIEGQVVAGTINYRVADNYFLEVIAHDPRYNAYRLG
                     TLCCYLTVCECIARGGREYHFLWGQDEYKKRLLGKRRDLDDLTVYRSRLHMVSHADLA
                     LRQAADGWKRRAQLRIRTLRQGDGPAARTFDALLRRIREMKSGG"
     gene            complement(4823..6943)
                     /locus_tag="VM406_03140"
     CDS             complement(4823..6943)
                     /locus_tag="VM406_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVL74985.1"
                     /translation="MPDPLPPSPPSASPPGLPGEAARALLAQFQGMVYRCRIDSAWTL
                     EFISEGCTRMTGYAPDDFTLRGLITYEGLIHPEDRERVRAQVREALAADGHFSLEYRI
                     ICRDGSVRWVGEQGKVIADGHRHPVALQGFVQDITARIESERALREAERRYRGIFENA
                     VEGMYRSTPEQGFLDVNPALARICGYGSVTELLEAVRDIANQLYADPRRRAALIGQLQ
                     QFGEVRSFESEIKRRDGSRTWVSENARVVRDACGNRMVIEGTVSDISERREYEARIRH
                     QATHDALTGLPNRMLLLDRLQQAVYAAKRSDTRVAVVFVDLDQFKFVNDSLGHEAGDM
                     LLARMAARLRATVRACDTVSRHGGDSFVLVLTACVSLEAVQVAVRRIMAALAEPLEIG
                     AAEIQVTCSVGISLYPDHESSPDALLRQADSALYKAKEAGRNSYAFFDARTRLDASHR
                     LALLNSLRHALVQQQFRLHYQPRIDLASGALVGAEALIRWLHPSRGMVPPAQFIPVAE
                     DSGMILAIGEWVLRTACAQNKAWQEAGMAPIPVSVNLSPRQLAHEHFVDLVADALAAS
                     GLEARYLELEITETLVMRDVEQSRSVFERLKELGVGISIDDFGTGYSSLNYLKRFPVD
                     TLKIDRSFIRDVAVDSDDAGIAMAVISLGRVLQLQVLAEGVETEAQRQFLLENGCHHA
                     QGYLFAHPLEPDEFAARFLVPGPR"
     gene            7172..8017
                     /locus_tag="VM406_03145"
     CDS             7172..8017
                     /locus_tag="VM406_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVL74986.1"
                     /translation="MSYKTILVFADESRHAQKRFEIAARLALQEDAHLAGVAATAMPG
                     AFYLPEMIGESTMSLTAYLEYLRQRADGVLAQFEGTARNAGVPGYERRVVDDEAASAL
                     CLQARYADLVVIGQTDPDEQLPALRQGFPEYVVLNAARPVLVIPYAGDFPQIGKRIVV
                     AWDASTEATRAITAALPFLRRADVVQVTVFDAGARGLTHGEQPGADIALFLSRHGVKV
                     EVSQQVTADRIDVGNALLSAAADFNADMMVMGCYGHSRFREVLLGGVSNTIFRSMTVP
                     VLMCH"
BASE COUNT         1285 a         2716 c         2748 g         1372 t
ORIGIN      
        1 ggcggcggcc accgcgaaac ggccggccag gctggtcggg gcaagggcgc gccgcttcat
       61 tgctccaccg gcacgaggat gccgtcctcg cgatagcggg tcatcagcag ttccgccggc
      121 cccagcgcgt catgacgcgt cggcgtgaat ggcggcgagt agcgcttgat cagcccgtcg
      181 taggatccca ggcgctcgag cgcatcgcgc acggcttcgc gccgggtact gccggcctgt
      241 tcgaccgccc gtgcaacgat gtgggtggcg tcataggcgt gcgccgctcc gaccggggcg
      301 tcgatgttct cgatgcggcg aaccctgccc tcgctcactt gcgcagcggt gcgcaggaaa
      361 cgctggaccg cgcccggatc cgccctgaaa aagctgaagg tctggatcac gctcaggtcg
      421 acctcgcgca gcgccgcgcc ggcctgggta aaaaactcgc cgccggtaat gccccagtgg
      481 ctgatgatgg gcacgcggcg ctgccgcggc aggccagcca cctcgcgaac cagggttgcg
      541 ccctcctcat cgttgccgac caggatgacg gctcgggcac cgctgtcaac gagtccgagg
      601 tacttctcga tgaagctgcg atcggcggcg ttgaaccacg cgacggcggc gatggccggc
      661 ttgcccccct gccgcacgtg ctgttcggca gcggcaagat tgctgcgccc ccaggacgta
      721 ttggtcagca acatgccgac cggggccaag ccccgggccg aagcggatga cagcagcttg
      781 ggcatggcga tgctgtcgcg caacgacaga cggaatacat agttgggctg catgccgttg
      841 tcggtaatca tgtcggccga cgaccatacc gccatgaagg gcagccgcag tttcgcgatc
      901 agcggcagtt gctcgatcac gaccgggctg aaacggccgc cgaacacggc caccaggtcg
      961 ggcagggcgg ccagctgctc gagattgcgc acgccgcgcg ccggtatcga gcggtggtca
     1021 cgcgtgacca gctgcagcgg tcgtccgccc agcaccccgc cggcgcggtt gatttcatcg
     1081 atcgccaccc gcatcccgag ttcaatcgct tgggccgatg tactgtgtgc aaggccgaat
     1141 tcggcatcga gaccgagcag gacaggtgat gcggccgcca gcggaagcgg aagcgccgca
     1201 cccgccgctg ccgccacaag cgcgcgcacc accgtgcgac gagtcaggtc gggccggccc
     1261 gcgcctcccg cttcgttccg cgcaccccgc gcagaatcca tgccggggcc ggcatcatcg
     1321 gtcgatctgc gcaacacgct cgcggaggca gggtggatca tgggcagtct ttcggaacat
     1381 gacgggaatc cggccgaacg tccatcatag ccgcagttcc ggcggccgag atggcgttgc
     1441 gcaacggtct gctgcgcttc ggcattactg tgcacacatc ggaggtgagc atgacgatcc
     1501 tggttctcgg cctggtgctg tttctcggta tccatctggt tcccacgtcc cccgcgctgc
     1561 ggatgcagct cgccggccgc ctcggggagc acggctatcg cggcggattt tccgccatgg
     1621 cggcgatcgg gctgatcctg atcgtggtcg gctacggcat cgcgccgccc ggtccgcagc
     1681 tgttcgagcc gctccatgcg gcgcgcgtat gggcgccggc ggccatggtg ctgagcttca
     1741 tcctgctggc ggcagccaac atgcgcaccc atctgcgtcg cgtgctgcgt catccgatgc
     1801 tgatcggcgt gggcctgtgg gcgctcgtgc acctgctcgc caacggcagc ctgcgcgcga
     1861 ccattctgtt cggagcgttt ctcgcctatg tggttctcga tttcgcgtcg gccaccgcgc
     1921 ggcacgcggt caagacattc tcgccggcct tcagccagga tgtgatcgcc gtcgccgccg
     1981 gcacggtgct cgccctgctg gtcatggcgc tgcatggctg gctgttcggt gtggcggtga
     2041 tgccgtttcc tggctgaacg caacccttca tctttctcaa gggacggcgc ttgcgctgga
     2101 acgggaggcc ggcgtcgcat gtcctagttc tgccgtgcta cgcggcagga cggaggtctc
     2161 gtgcgccggc gcgcgttact gatcattaat cgcaacagcc gcagcggcgc ggaggacgca
     2221 gagcgcatac gcgcgcgcct ggcgggctgc ggcatcgagg tgttcgagcc gaaaccgacg
     2281 ctgcatgccg aaatctgcga tgtcatccgc aaccatcggc agaaggtgga catgatcgtc
     2341 gtgggcggcg gcgacggttc gctcaatgcg gtggcgccgg ccctggtcga atgcggtctg
     2401 ccgctgggcg tgattccgct gggtacggcc aacgacctcg cgcgcacgct gtcgatccca
     2461 cccgatcccg atcaggcctg catgatcatc ggcgcaggat gccggcaccg catcgacctg
     2521 gggcgcgtga acggccgcta tttcttcaac gtcgcgcaca tcggcctggg cgcacgggtc
     2581 acgcataccg tgtcggggcc ggccaagcgc cagctgggcg ccttcgccta cttcgccgcg
     2641 ctcgcacggg cgctgcgctc gacgcgcacc ttccatgcgc tgatcgactg cgacggtcgg
     2701 cggctggaga tggacgcggt gcagatcgcc gtgggcaacg gccgctactt tggcggcggc
     2761 acgacggtcg ccgcggccgc tgcgatcgac gacggccgct ttttcctgtg cggcgtcgaa
     2821 ccgctcggct ggcgcgaggt gattcgcatg gcgctgcgcg cacgggcgct gtacgccggg
     2881 cggttcgagg agcgcgatcc ggtacaggtc gaacaggggc gcgagatctt cgtgcacacg
     2941 cgccgcccga tgatcatcag cgccgacggc gaactggtcg ggcgcacccc cgcccgcttc
     3001 gagcttctcg agcgcgcggt cgaggtgttc gtgccgcagg cctatctgga aaacgaggtg
     3061 aagcatgttg ctcagggatg aaagacaggc ggcgctcaat cgcgtcgaaa cgctgtgcat
     3121 caacagcgcc gacgactacg cgtccgccgc cacgcagacc gatgatgcgg cgctggccgc
     3181 gctgttcgag cagcttggtc aggagcgccg cggcatggcc gaggcgctgg cgccgcacat
     3241 ccgcgcgctc ggggaccttc cgcaggaccc ggatccggac cgcgaggcgc tcgaccacgt
     3301 cgtggtagga ctcaaggccc tgctgtccgg agatggcggc gccacgctgg tcgaacaccg
     3361 gctgcgcgcg gaggatgcac tgcgcgaggc cgtgctcgca gcgcaggatg cggatctgcc
     3421 ggaacacacg catgcggcgc tcgccggcat gctggcgggg atcgaccgcg cgcgtgcccg
     3481 cctgctcgaa atacgcgcct gagaagcgcg cggccgttca gcccccgctc ttcatttcac
     3541 ggatgcggcg cagcagcgcg tcgaacgtgc gcgccgccgg tccatcgccc tgtcgcagcg
     3601 tgcgtatgcg cagttgcgcc cggcgcttcc agccgtcggc ggcctggcgc agcgccaggt
     3661 cggcatggga caccatgtgc aggcgcgagc ggtacacggt caggtcgtcc aggtcgcgcc
     3721 gcttgccgag cagccgtttc ttgtattcgt cctgccccca gagaaagtgg tactcgcggc
     3781 cgccgcgcgc aatgcactcg cataccgtca ggtagcagca cagggtgccg agccggtagg
     3841 cgttatagcg gggatcgtga gcgatcactt ccaggaagta gttgtcggct acccggtaat
     3901 tgatcgtgcc ggcgaccacc tgtccttcga tggtgagcgc gccgatcatc ccgcagtcca
     3961 ttgcaaggtg ggtgatctgc tcggccagcg tgtcgtcgtt gatcgatacc ttgcccttgt
     4021 gcgacatgcg cgcccgattg agttcgatga tgcggcggat cttgccctcg tcgatctcgt
     4081 gcgcctcgac gagctcgaag cgcaccgacg ggaagtcgcg gcgcagcttg ttcatgtagc
     4141 gatggatgta gctgcgcgtg gacttgccga gctgggccag gtactcggtc gaggtcggcg
     4201 gcagttcgag caccatgtcc tcgaggcagt tgtagcgccg ccagggcagt gccaggcctt
     4261 gcacgcgcgg ctggatcgca tgaaaggtga tcgcgccggc cgccgagccg ggggcgaaga
     4321 ccagctcggc gaatcgggcg acttcatgcg cgtggacctc gatgccttcg ttgagcacgc
     4381 ggacggtacg cccgcgccgt tcatacagcc agacggtgcg cagctgtgtg ccgtcgcgca
     4441 gcacgaaagt gctcaggctg cgcgacttgc cgtagacacg ctggtgcgcc agtgtggaaa
     4501 acagattgcc gtacaagccc tcgagcgcct cctccacgaa aggcggcacc gcgtgctcga
     4561 agcattcggc ggtcagggcg ccgctggccg ccgtgcgcgc attcgacctg tccagcgcag
     4621 cgatatttcc ggccgctgtt tcagtggtga tcatgactcc tgctccatcc ctttgcgttc
     4681 gcactcgacc cgcgacgcag tctggcgccg ctggtctaag gttggattgc cgatgcttca
     4741 ggagttcatc tcgccacggg aacgccaatg tctcgtttgt gccagcgcaa acggcgcgta
     4801 ttccgggttt cccgcagggg attcagcgcg ggccgggaac gaggaagcgg gccgcgaact
     4861 cgtccggctc cagcggatgg gcgaacagat agccctgcgc atggtggcag ccgttctcca
     4921 gcaggaattg gcgctgcgcc tcggtttcga caccttcggc cagcacctgc agctgcagca
     4981 cgcgaccgag cgagatcacc gccatcgcga tgcccgcatc gtcgctgtcg acggccacat
     5041 cccggatgaa cgagcggtcg atcttgagcg tgtcgaccgg gaagcgcttc aggtagttca
     5101 ggcttgagta gccggttccg aagtcgtcga tcgaaattcc cacgccaagc tccttgagcc
     5161 gttcgaacac cgagcgcgac tgctcgacgt cgcgcatgac cagcgtctcg gtgatttcca
     5221 gctcgagata gcgcgcctcc agacccgacg cggccagtgc atcggcgacg aggtcgacga
     5281 agtgctcgtg ggcgagctgg cgcggcgaca gattcaccga taccggtatc ggcgccatgc
     5341 cggcttcctg ccaggccttg ttctgcgcac aggccgtcct gagcacccat tcgccgatcg
     5401 caaggatcat gccgctgtct tcggcgaccg ggatgaactg cgcgggcggc accatgccgc
     5461 gcgacggatg cagccagcgg atcagggctt cggccccgac cagcgcgccg ctggccagat
     5521 cgatgcgcgg ctggtagtgc aggcggaact gctgctggac gagcgcatgg cgcaggctgt
     5581 tgagcagggc cagccggtga ctggcgtcaa ggcgggtgcg ggcgtcgaag aacgcatagc
     5641 tgttgcgccc cgcctccttg gccttgtaca gcgccgagtc ggcctggcgc agcagcgcgt
     5701 cggggctgct ctcgtggtcc ggatacaggc tgatgccgac gctgcaggtc acctggatct
     5761 cggccgcgcc gatctccagc ggctcggcga gggcggccat gatgcggcgc accgccacct
     5821 gcaccgcttc gagcgagacg caggccgtca gcaccagcac gaacgagtcg ccgccatggc
     5881 gcgagacggt atcgcaggcg cgcaccgtcg cgcgcaagcg cgccgccatc ctggccagca
     5941 gcatgtcgcc ggcttcatgg ccaaggctgt cgttaacgaa cttgaactgg tcgaggtcga
     6001 cgaacaccac cgccacgcgc gtgtcgctgc gcttggccgc atacacggcc tgctgcaggc
     6061 ggtcgagcag cagcatgcgg ttgggcagcc cggtcagcgc gtcgtgggtg gcctggtggc
     6121 ggatgcgcgc ttcgtattcg cgccgttcgc tgatgtcgct gaccgtccct tcgatgacca
     6181 tccggttgcc gcatgcgtcg cgcacgaccc tggcgttctc cgacacccag gtgcggctgc
     6241 cgtcgcggcg cttgatctcg gattcgaaac tgcgcacttc accaaactgc tgaagctgtc
     6301 cgatcagcgc ggcgcgccgg cgcgggtccg catacagctg gtttgcgatg tcgcgcacgg
     6361 cctccagcag ttccgtcacg ctgccgtacc cgcagatgcg ggccagcgcc ggattcacat
     6421 ccaggaagcc ctgttcaggc gtggaccggt acatcccttc caccgcgttc tcgaagatgc
     6481 cgcggtagcg ccgctccgct tcgcgcagcg cccgttccga ctcgatgcgc gcggtgatgt
     6541 cctgcacgaa tccctgcagc gcgaccggat gccggtgtcc gtcggcaatg accttgccct
     6601 gctcccccac ccagcgcacc gagccgtcgc ggcagatgat ccggtactcg aggctgaaat
     6661 gcccgtcggc ggcgagcgcc tccctgacct gcgcgcgcac gcgctcccgg tcctccgggt
     6721 gaatcaggcc ctcgtaggtg atcaggcccc ggagcgtgaa atcgtccggc gcgtagccgg
     6781 tcatccgggt gcagccctcg ctgatgaatt ccagagtcca ggcgctgtcg atcctgcagc
     6841 ggtagaccat cccctggaac tgcgcgagca gcgcgcgcgc agcctcgccc gggagtccgg
     6901 gtggtgaagc cgaaggcggc gaaggcggca gcggatcagg catgcgggag acgtgggaca
     6961 ttattgccgt agggcaacat gatgccatgc cggactttga ttgtaaaaaa tgtgtgacaa
     7021 cgagaagcag tgtttcagcg gtttgacatg cgccgccaac ggctgggcgc cgaattgacc
     7081 tggatcaagg gaaatggaag gtgtggcaac taacctgcaa taacaacctg gttccgcgcc
     7141 gaaacggcgc ggatctgacg aaaggaagag catgagctac aagaccatac tggttttcgc
     7201 cgacgaatcg cgccacgccc agaagcgctt cgagatcgcc gcgcgcctgg cgctgcagga
     7261 agacgcgcac ctggccggtg tggccgccac cgcgatgccg ggcgccttct acctgcccga
     7321 gatgatcggc gagagcacga tgagcctgac cgcctatctc gagtatctgc gccagcgcgc
     7381 cgacggcgtg ctcgcgcagt tcgagggcac cgcacgcaac gccggcgtgc cgggctacga
     7441 acggcgcgtg gtcgacgacg aggcggcgtc ggctctgtgc ctgcaggcac gctacgcgga
     7501 cctggtcgtg atcggccaga ccgatccgga cgagcagctg ccggcgctgc gccagggttt
     7561 tccggagtac gtggttctca acgccgcgcg cccggtgctg gtgattccct acgccgggga
     7621 ctttccgcag atcggcaaac gcatcgtggt ggcctgggac gccagcaccg aagccacgcg
     7681 cgcgatcacc gccgcgctgc cgttcctgcg ccgcgccgac gttgtccagg taacggtgtt
     7741 cgatgccggc gcacgcgggc tgacccatgg cgagcagccc ggcgccgaca tcgccctgtt
     7801 tctgagccgg cacggcgtca aggtcgaggt atcgcagcag gtgaccgctg accgcatcga
     7861 tgtcggcaac gcgctgctct ccgccgccgc cgacttcaac gccgacatga tggtgatggg
     7921 ctgctacggc cattcgcgct tccgcgaagt gttgctgggc ggcgtatcga acaccatctt
     7981 ccgctcgatg acggtgcccg tgctgatgtg ccactgacat cctgcaacag gcccgcgcgc
     8041 cggagcacag cgcgcgcgtt ctcgactacc attgagggtt gccccagttt ccaggcccct
     8101 catgtcttca gacctgttca a
//
LOCUS       DATLGF010000106         3301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_224286, whole genome shotgun sequence.
ACCESSION   DATLGF010000106 DATLGF010000000
VERSION     DATLGF010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224286"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /gene="gatB"
                     /locus_tag="VM406_03150"
     CDS             complement(<1..1213)
                     /gene="gatB"
                     /locus_tag="VM406_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HVL74987.1"
                     /translation="MQWEVVIGLETHAQLSTESKIFSGASTRFGAAPNTQASPVDLAL
                     PGVLPVLNKGAVERAIQFGLAIGATVAPRSIFARKNYFYPDLPKGYQISQYEIPVVQG
                     GTISFVLEKDGKAEMKTVQLTRAHLEEDAGKSLHEDYHGMSGIDLNRAGTPLLEIVTE
                     PDMRSAAEAVAYAKALHALVVWLGICDGNMQEGSFRCDANVSVRPLGQQEYGTRCEIK
                     NLNSFRFLEEAINYEVRRQIELIEDGGKVVQETRLYDPDRKETRSMRSKEDAQDYRYF
                     PDPDLPPLVIANEWIERVKAAMPELPGAMRERFVRDYGLPEYDAAVLTQSKAMAAYFE
                     AVLAKAGHEQAKPAANWLMGDVSSTLNREGVDIADAPVKAEQLALLLQRIADGTISNK
                     IAKEVFGAMWEA"
     gene            complement(1282..1758)
                     /locus_tag="VM406_03155"
     CDS             complement(1282..1758)
                     /locus_tag="VM406_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74988.1"
                     /translation="MRALPAALLALAWSAPLAAAQQLDARFSCSDKQEVAGEQLTFAD
                     HGRIRLSGDRIEAFRWESALYRSTHGFDCNIDEEDGLKAELTGQAAWRIELRDPLEAR
                     MRRGYDFARHMVCTVRLEREGDTVHIRPTCPALCGSRNNFSELSVNTKTGECQYQH"
     gene            complement(1755..3215)
                     /gene="gatA"
                     /locus_tag="VM406_03160"
     CDS             complement(1755..3215)
                     /gene="gatA"
                     /locus_tag="VM406_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HVL74989.1"
                     /translation="MHTKTIKELSTLLQSRQISATDLARLYLDRAKNSDLNAYIDINE
                     ALTLQQAEEADRRLAGNDATPLTGIPIAHKDIFVTRGWRSTAGSKMLSGYTSPFDATV
                     VEQFHKAGTVTLGKLNCDEFAMGSSNENSFFGPVKNPWDKAAIPGGSSGGSAAAVAAR
                     LAPGVTATDTGGSIRQPAAFCGVTGIKPTYGRVSRFGMIAFASSLDQGGPMAQTAEDC
                     ALLLNAMAGFDPKDSTSLERPAEDFTRELNAPLSGLKIGVPREYFGEGLADDVEQAVR
                     AALAEYEKLGATLVDISLPNTKLSIPAYYVIAPAEASSNLSRFDGVRYGHRAKDYVDL
                     ADMYRKTRAEGFGEEVKRRILVGAYVLSHGYYDAYYLQAQKIRRLIAQDFQQAFAQCD
                     VIMGPVAPSVAWNLGDKADDPVANYLADIFTLSTSLAGLPGMSVPCGFGKGERNGKRP
                     VGLQIIGNTFQEARLLNVAHQYQLATDWHTRCPEGV"
BASE COUNT          528 a         1028 c         1101 g          644 t
ORIGIN      
        1 gggcttccca catcgcgccg aacacttcct tggcgatctt gttcgagatg gtaccgtcgg
       61 cgatgcgctg cagcagcaac gcgagctgtt cggccttgac cggcgcgtcg gcgatgtcca
      121 cgccttcgcg gttcagcgtc gaggacacat cgcccatcag ccagttcgcc gccggcttgg
      181 cctgttcgtg gccggccttt gcaagcaccg cctcgaaata ggcagccatc gccttcgact
      241 gcgtcagcac cgccgcgtcg tactcgggca ggccgtagtc gcgcacgaag cgctcgcgca
      301 tcgcgccggg cagctccggc atcgcggcct tcacgcgctc gatccattcg ttggcgatca
      361 ccagcggcgg caggtcgggg tcggggaagt agcggtagtc ctgcgcgtct tccttgctgc
      421 gcatcgagcg ggtttccttg cgatccgggt cgtacaggcg ggtttcctgc accaccttgc
      481 cgccgtcctc gatcagctcg atctggcgcc gcacttcata gttgatcgcc tcttccagga
      541 accggaacga gttcaggttc ttgatctcgc aacgcgtgcc gtattcctgc tggccgagcg
      601 ggcgcaccga gacattggcg tcgcagcgga acgagccttc ctgcatgttg ccgtcgcaga
      661 tgcccagcca cactaccagc gcgtgcagcg ccttcgcgta ggcgacagct tcggcggcgc
      721 tgcgcatgtc gggctcggtg acaatctcaa gcagcggcgt gccggcgcgg ttcaggtcga
      781 tgccgctcat gccgtgatag tcctcgtgca gcgacttgcc ggcgtcttcc tccaggtggg
      841 cgcgggtcag ctggaccgtc ttcatctcgg ccttgccgtc cttttccagc acgaaggaaa
      901 tcgtgccacc ctgcacgacc gggatttcgt actggctgat ctggtagccc ttcggaagat
      961 ccgggtagaa gtagttcttg cgcgcgaaaa tcgagcgcgg cgcaaccgtt gcgccgatcg
     1021 caagaccgaa ctggatcgcg cgctcgaccg cccccttgtt cagtaccggc agcacgcccg
     1081 gcagcgccag gtcaaccggg ctggcctggg tgttcggcgc ggcgccgaag cgggtggagg
     1141 cgccgctgaa aatcttcgat tcggtggaga gctgggcatg cgtttccagg ccgatcacta
     1201 cttcccactg catggtgtcc tcgttcgttt tttgtccgga ccgccccgcc gttgcaggcg
     1261 ggaaggaagc cgggcgcggt ttcagtgttg gtattggcac tcgccggttt tggtattcac
     1321 cgacagttcc gagaaattgt tgcgcgagcc gcacagcgcg gggcaggtcg ggcggatatg
     1381 caccgtgtcg ccctcgcgtt ccagccgcac cgtgcagacc atgtggcggg cgaagtcgta
     1441 gccgcggcgc atgcgcgcct cgagcggatc acgcaattcg atgcgccagg cggcctggcc
     1501 ggtgagctct gccttcaggc cgtcttcctc gtcgatgttg cagtcgaagc cgtgggtgct
     1561 gcgatacagc gcggattccc agcggaaggc ctcgatgcgg tcgccggaca gacggatgcg
     1621 gccgtggtcg gcaaaggtga gctgttcgcc cgcgacctcc tgcttgtcgc tgcacgagaa
     1681 acgtgcgtcg agctgttggg ccgctgccaa cggcgcggac catgccaggg cgaggagggc
     1741 ggcgggcaag gctctcatac gccttccgga cagcgcgtgt gccagtcggt ggcgagctgg
     1801 tactggtggg cgacgttcag caagcgggcc tcctggaagg tgttgccgat gatttgcagt
     1861 ccgaccggac gcttgccgtt tcgttcaccc ttgccgaagc cgcaaggcac cgacatgccg
     1921 ggcaggccgg cgaggctggt cgacagcgtg aagatgtcgg ccagatagtt ggccaccgga
     1981 tcgtcggcct tgtcgccgag attccaggcc acggagggcg ccaccggccc catgatcaca
     2041 tcgcattgcg caaatgcctg ctggaagtcc tgcgcgatca ggcggcggat cttctgcgcc
     2101 tgcaggtagt aggcatcgta gtagccgtgc gagagcacgt aggcgccgac caggatgcgg
     2161 cgcttgacct cttcgccgaa gccttcggcg cgcgtcttgc ggtacatgtc ggccaggtcg
     2221 acgtagtcct tcgcgcggtg gccgtagcgc acgccgtcga agcgcgacag gttcgacgag
     2281 gcttcagcag gcgcgatcac gtagtaggcc gggatcgaca gtttcgtgtt cggcagcgag
     2341 atgtcgacca gggttgcgcc cagcttttca tattcggcca gcgcggcgcg taccgcctgc
     2401 tccacgtcgt cggccaggcc ctcgccgaag tattcgcgcg gcacgccgat cttgagtccg
     2461 gaaagcggcg cgttcagctc gcgcgtgaag tcttcggccg ggcgttcaag gctggtcgag
     2521 tccttcggat cgaagccggc catcgcattg agcagcagcg cgcaatcctc cgcggtctgg
     2581 gccatggggc cgccctggtc cagcgaagat gcaaaagcga tcatgccgaa gcgcgacacg
     2641 cggccgtagg tcggcttgat gccggttacg ccgcagaaag cagccggctg gcgaatcgag
     2701 ccgccggtgt cggtggcagt gacgccgggc gccaggcggg cggcaacagc cgcagcggaa
     2761 ccgcccgacg agccgcccgg aatcgcggcc ttgtcccagg ggttcttcac cggtccgaag
     2821 aacgaattct cgttcgagga gcccatggcg aattcatcac agttgagttt tccgagcgta
     2881 acagtgcctg ccttgtggaa ctgttcgacg actgtcgcat cgaaggggct cgtgtagcct
     2941 gagagcatct tcgacccggc ggtcgagcgc cagccgcgcg tgacgaagat gtccttgtgc
     3001 gcaatcggta tgccggtcaa gggcgtggca tcgttcccgg ccaggcgccg gtcggcttct
     3061 tcagcctgct gcagggtcaa ggcttcgttg atgtcgatgt aggcattcag atcactgttc
     3121 tttgccctgt cgagatagag gcgagccagg tccgtcgccg agatttgccg gctttgcagc
     3181 agagtggata gttctttaat ggtcttggta tgcatggcgt gattaggcat tcaacttctg
     3241 cgcgaattgg cgcttgcagg cgcagaaaac gcgaaaaatt ttttccgcac gggaattatt
     3301 c
//
LOCUS       DATLGF010000107         8652 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_131525, whole genome shotgun sequence.
ACCESSION   DATLGF010000107 DATLGF010000000
VERSION     DATLGF010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 8652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8652
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131525"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VM406_03165"
     CDS             complement(<1..223)
                     /locus_tag="VM406_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVL74990.1"
                     /translation="MTSVRVERAGRVTTVILDRAAARNAVDRATADALAAAFRTFEAD
                     DGSDVAVLWGAGGNFCAGADLKALDDPDRR"
     gene            complement(220..567)
                     /locus_tag="VM406_03170"
     CDS             complement(220..567)
                     /locus_tag="VM406_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL74991.1"
                     /translation="MRVKLVQIAHARGGDKGDKADLGLFAYDVAGYEVIRREVSAERV
                     AAHFAGIARGRVECFPLDNLLALKFVLHEALDGGAARSLRSDNLGKSLAAGLLRMEIE
                     VPDTLPLRHEELL"
     gene            complement(569..1954)
                     /locus_tag="VM406_03175"
     CDS             complement(569..1954)
                     /locus_tag="VM406_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HVL74992.1"
                     /translation="MKTVRIGAGLGFYGDAWAPVAASVERGGVQYIASDHLAELTLAI
                     LQKDRAKDPAAGYARDLVPMLARLWPLAQKNGGVKFLLNAGGLNPHGARDALAELFRK
                     KGWHARIAVVTGDDVIDRLDALRAQGDALAHMDTGVALETVRERMVFANAYLGAAPLV
                     RALEQGADIVLCGRVADAALFLAPLVHEFGWTLDARSDAQRNLLAQGLTVGHLLECSG
                     QGSGGNFGGLDWQEIPDLAHIGYPIAEVSEDGCAVITKAPGTGGRVSFDTVREQLLYE
                     VQDPHNYFSPDVVLDMGGLRVDDLGDDRVRVTGAIGKPRPQKLKIVGGYSDGWMGQAT
                     FGYSWPQAYAKAERSAQIIRQTLAEQRMPVDEIRVEYLGYDSILGPLADPAQRGQLNE
                     VYLRMAIRSQDRRVADGFGRLFPWLALSGPPFVGGRFAMQPASELLGIWPTLAAREAI
                     EDAVRVDVMEV"
     gene            complement(1962..2741)
                     /locus_tag="VM406_03180"
     CDS             complement(1962..2741)
                     /locus_tag="VM406_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVL74993.1"
                     /translation="MTTRLTIADGLATVVFDDAATLNAMGPKQIADMNRVIAALADDA
                     SVRVVLLRAEGPAFGVGGDLRAFNPKTGNAAAALREIGRDLNPMILRLRTLPAIVVAA
                     VHGAVAGGSMGLMCAADIVLVAEGTKFSTAYARIAASPDAGNSWFLPRLVGQRKALEW
                     LLLSDTFDAATAHAFGLVNQVVPAAQLAAESERIVARLLAGPHSSHARIKRLVYQSET
                     TPLPVQLDAEIEHFAQCAEGPDFAEGVAAFVEKRAPRFNRS"
     gene            complement(2738..3820)
                     /locus_tag="VM406_03185"
     CDS             complement(2738..3820)
                     /locus_tag="VM406_03185"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HVL74994.1"
                     /translation="MPQTTPSDHDVRADEFHVRDALPATAATAAAARKRVPPEILARC
                     TRLDPVRSLLSTIDTFGFLLVLGVVGVAGGTLVWPAVLVVILMPAAQHRLAILSHEAT
                     HYRLFKTRALNDFTGWLASALIGISVYGYRVIHRIHHNHLYEKIDPDMPLQAGYPRGR
                     AYLFRKLGKDLLGVTAWKNYAYFFGAPAMNRDTGGRGGKNGDPLADTTPALRRQALVD
                     RWRVAAFQLALMGALLWAGWLHYYLVLWVLPLLTLYQAVLRLRAVCEHGAPGNTATPL
                     TAARTNVELPLLARFYLFPHHVNYHIEHHLYPAVPHYHLPRLHTELKQAGLLQGAEVR
                     AFRSTLARVFADPPPSAQPRTPRTAT"
     gene            4068..4958
                     /locus_tag="VM406_03190"
     CDS             4068..4958
                     /locus_tag="VM406_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVL74995.1"
                     /translation="MVGLQIALSGIAQGCIYGLIALGFVLIYKATETVNFAQGELMML
                     GAFIGLSCVTLLGLPFWLAIAVTMLAMLSFGMIMERSLLRPLLGQPAFTVVMVTIGFG
                     YLARGVVTMVPPWGTDTHVMPAPYKDELVRIGAEGGQQLVASMEQIVIIAATAMLCAV
                     LFLFFRFTRLGIAMQAASQNQLAAFYMGIPVRRLNSLVWGISAAVAAIAGILLAPITF
                     VHSNMGFIGMKAFPAAVVGGFGSIPGAIVGGLVIGLVESFSGYYLPEGFKDVAAYVVV
                     LIMLVVRPNGLFGEKLRKRV"
     gene            5016..6092
                     /locus_tag="VM406_03195"
     CDS             5016..6092
                     /locus_tag="VM406_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVL74996.1"
                     /translation="MRFLFKTDYNQDIRLLKHEGMRFWYGVLAVLLLLAPWLLTEYHL
                     SQLVFVLIYAIAALGLMLLSGFTGQVSIGHAAFLAVGAYTEAYLAARGWPFVFSLTAA
                     AGLSAITGIIIGLPALRTKGMYLAIATLAFGFIVEEILTRAEGITGGAGGMMVGRIVL
                     FGFEIDTETKFYFLSLAITVIVTLAVVNLLRSPTGRAFVAIRDSEISAQSMGINLARY
                     KTTAFALSAALAGIAGALYAHKIRFLTPEQFGFLQSIELLMIIVIGGLGFVHGAFFGA
                     AFWVLIQQLIVSFKGYLPDAIAHQTGLQPTIFGIILIAFVLFEPMGLYGRWLKIRTYL
                     ELFPFYRKGMFKRQKSYMKSERLR"
     gene            6089..6862
                     /locus_tag="VM406_03200"
     CDS             6089..6862
                     /locus_tag="VM406_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL74997.1"
                     /translation="MSTPLLQAENLTVKFGGLTAVNNVSFTVREGEIFTLIGPNGAGK
                     TTTFNLISGLYQPTSGTIRLDGETISGLAPYRIAEMGVARTFQNIELFEHATVLQNLL
                     IGRHTQRRTSLWQEIVFADAVRRAELDFRAEVEKVIEFLDLQHYRDSLVAGLPYGVRK
                     VVELARALSMQPRLLLLDEPSSGLNTEETDDMAFWIKDIQTDLGITVIMVEHDMSLVS
                     RVSDRVLALNYGEVLATGTAAEVQSHPAVIEAYLGGVEA"
     gene            6859..7629
                     /locus_tag="VM406_03205"
     CDS             6859..7629
                     /locus_tag="VM406_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL74998.1"
                     /translation="MSDILQLNNVESSYGPVKAIRGVSLKVKEGSIATVLGSNGAGKS
                     TILKTISGIIDPQKGTILFQGKPIQKKDPADIVRDGIVHVPEGREVFPLLSVYDNLLM
                     GSYTRSDRDAVARDIEAAYAYFPILKERAKQEAGLLSGGQQQMLAIARGLMAQPKVML
                     LDEPSLGLSPKLTKEIFEIIRRINAERGTTILLVEQNALVALNYADYGYVLEVGRIVM
                     EDTCAALREKEDIKEFYLGMKETGVRGERRWKRKKNWR"
     gene            7665..>8652
                     /locus_tag="VM406_03210"
     CDS             7665..>8652
                     /locus_tag="VM406_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVL74999.1"
                     /translation="MQHTIVDDTIIVPGDNIPRLFWNAVAKRRDKICLRQKEYGIWQS
                     MTYGEVAQVVSEIGYGLVALGFEPGERASILANTIREWFCADLAVLCAGGVSNGIYPT
                     DSPSQVEYLMQDSGSAYIFVEDEEQLDKVLEVRGSLPDLRKIIVFDMEGLRNLNDEQI
                     ISLDQLRALGRDYEAQDPSARAVEWESRLDARRPEDLAILIYTSGTTGRPKGAMISHH
                     NVITAVRGFNSVLPQQEDDERMCFLPLCHVAERLGGAYFALYSGARLNFVENPETIPE
                     NVREISPTIFLAVPRIWEKFYSGVMIALSEGTTLEKLAYKKAIEVGKEAATFL"
BASE COUNT         1570 a         2882 c         2726 g         1474 t
ORIGIN      
        1 tgcgccggtc cgggtcatcc agcgccttca ggtccgcgcc cgcgcagaaa ttgccgccgg
       61 cgccccacag caccgcgaca tcgctgccgt cgtcggcctc gaacgtgcga aacgccgcag
      121 ccagcgcatc ggcggtcgcg cggtccaccg cgttgcgcgc ggccgcacga tcgagaatca
      181 cggtggtcac ccggccggcg cgttccacgc ggacgctggt cacagcagct cctcgtggcg
      241 cagcggcaag gtatccggca cctcgatttc catgcgcagc aggccggccg ccagcgactt
      301 tccgaggttg tcgctgcgca gcgaacgggc ggcgccgccg tccagcgcct cgtgcaatac
      361 gaacttcagg gccagcagat tgtcgagcgg aaaacattcc accctgcccc gcgcaatgcc
      421 ggcgaaatgc gcggccaccc gctccgcgct aacttcgcgc cgaatcacct cgtaccccgc
      481 gacgtcatag gcaaacaggc caagatcggc cttgtcgccc ttgtcgccgc cgcgcgcgtg
      541 cgcgatctgc accagtttca cccgcatctc agacctccat cacatcgaca cgcacagcat
      601 cctcgattgc ctcgcgcgcg gccagcgtcg gccagatgcc gagcaactcg gacgccggct
      661 gcatcgcgaa acgcccgccg acgaacggcg gcccggacag tgccaaccat gggaacaggc
      721 gaccgaagcc gtccgcgacc cgcctgtcct ggctgcggat cgccatgcgc agatagacct
      781 cgttgagctg tccgcgctgc gccggatcgg ccagcggccc gaggatcgag tcatagccga
      841 ggtattcgac acggatctcg tcgaccggca tgcgctgctc tgcgagcgtc tggcggatga
      901 tctgcgcgct gcgctcggcc ttcgcgtacg cctgcggcca gctgtagccg aacgtcgcct
      961 gtcccatcca gccgtcgctg tagccgccga cgatcttcag tttctgcggg cgcggcttgc
     1021 cgatggcgcc ggtcacgcgc acgcggtcgt cgccgaggtc atcgacgcgc aggccgccca
     1081 tgtcgagcac cacgtcaggc gaaaagtaat tgtgcggatc ctgcacctcg tacagcagct
     1141 gctcgcgcac ggtatcaaag gacacgcggc cgccggttcc cggcgccttc gtgatcaccg
     1201 cgcagccgtc ctcgctgacc tcggcgatcg gatagccgat atgggcgaga tccggaatct
     1261 cctgccagtc gagtccgccg aagttgccgc cgctgccctg ccccgagcat tcgagcagat
     1321 ggcccacggt cagaccctgc gcgagcaggt tccgttgcgc gtcgctccgc gcatcgagcg
     1381 tccagccgaa ctcatggacc agcggcgcca ggaacagcgc cgcgtcggcc acccgtccgc
     1441 acagcacgat gtcggcgccc tgctccagcg cgcgcaccag cggcgccgcg ccgaggtagg
     1501 cgttggcaaa caccatgcgt tcgcgcaccg tctccagcgc caccccggta tccatgtggg
     1561 cgagcgcgtc gccctgtgcg cgcaacgcgt ccagccgatc gatcacgtcg tcaccggtca
     1621 ccaccgcaat gcgcgcatgc cagcctttct tgcgaaacag ctcggccagc gcatcgcgcg
     1681 cgccgtgggg attgaggccg ccggcattga gcaggaactt cacgccgccg ttcttctgcg
     1741 cgagcggcca caatcgcgcg agcatcggga cgaggtcgcg cgcatagccg gcggccggat
     1801 cctttgcgcg atccttctgc aggattgcca gcgtcagctc ggcgagatga tcggatgcga
     1861 tgtactgcac gccgccgcgt tcaacgctgg cggcaaccgg tgcccacgca tcgccgtaga
     1921 agccgaggcc tgcgcctata cgtaccgtct tcatcgccgc ctcaggaccg gttgaagcgc
     1981 ggcgcgcgct tttcgacgaa tgccgccacg ccctcggcga aatccggccc ttcggcgcac
     2041 tgcgcgaaat gctcgatttc ggcgtccagc tgaaccggca acggcgtggt ttccgactgg
     2101 tagacgaggc gcttgatccg cgcatgcgag ctgtgcggtc cagccaggag gcgcgccacg
     2161 atgcgctcgc tttccgccgc gagctgcgcc gcgggcacga cctgattgac gagaccgaag
     2221 gcatgcgcgg ttgcggcatc gaaggtatcg gagagcagca gccactccag cgccttgcgc
     2281 tggccgacca ggcgcggcag gaaccagctg ttgccggcat cggggctcgc ggcgatgcgc
     2341 gcgtaggcgg tcgagaattt cgtcccttca gccacaagca cgatatcggc agcgcacatc
     2401 agtcccatgc tgccgccggc gaccgcgcca tgcacggccg cgacgacgat cgccggcagc
     2461 gtgcgcagcc gcaggatcat cggattgaga tcgcggccga tttcgcgcag cgccgccgcc
     2521 gcattgccgg tcttaggatt gaacgcgcgc aggtcgccgc cgacgccgaa cgccggtcct
     2581 tcggcgcgca gcagcaccac gcgcacgctc gcatcgtcgg cgagcgcggc gatcacccga
     2641 ttcatgtcgg caatttgctt tggccccatc gcattgagcg tggcggcatc gtcgaacacg
     2701 acggtcgcaa gaccgtcggc aatcgtgagt cgtgtggtca tgtcgcggtc ctgggcgtgc
     2761 ggggttgagc ggaaggcggg ggatcggcga acacgcgcgc cagcgtcgaa cggaacgcgc
     2821 gcacctcggc gccctggagc aggccggcct gcttcagttc cgtgtgcagg cgcggcagat
     2881 ggtagtgcgg gacagccggg taaagatgat gctcgatgtg atagttgacg tggtgcggaa
     2941 agagatagaa acgcgccagc agcggcagtt cgacattggt gcgtgccgcc gtcagcggcg
     3001 ttgcggtgtt cccgggcgcg ccgtgctcgc ataccgcgcg caggcgcagc acggcctggt
     3061 acaaggtcag cagcggcagg acccagagca ccaggtagta atgcagccat ccggcccaga
     3121 gcagcgcgcc catcagagcg agctggaacg cggccacccg ccagcggtcg acgagcgcct
     3181 gccggcgcag cgcgggcgtc gtgtcggcaa gcggatcgcc gttcttgccg ccgcgtccgc
     3241 cggtgtcgcg gttcatcgcc ggcgcaccaa agaagtatgc gtagttcttc catgcagtga
     3301 caccgagcag atccttgcca agcttgcgga acaggtaggc acgtccgcgc ggataaccgg
     3361 cctgcagcgg catgtcggga tcgatttttt catacaggtg attgtgatgg atgcggtgga
     3421 tgacccgata gccgtacacc gaaatcccga tcagcgccga ggccagccat ccggtgaaat
     3481 cgttcagcgc gcgcgtcttg aacaggcgat agtgggtcgc ctcatgcgaa aggatcgcga
     3541 ggcgatgctg cgcggccggc atcaggatca ccacgaggac ggccggccac accagcgtcc
     3601 cgccggccac acccacgacc ccgagcacga gcaggaatcc gaaggtgtcg atcgtcgaca
     3661 gcagcgagcg caccggatcg agtcgcgtgc agcgcgccag gatttccggc ggcacgcgct
     3721 tgcgcgcagc ggcggctgta gcggcagtag caggcagggc atcgcgaacg tgaaactcgt
     3781 ccgcacgtac gtcatgatcg gagggagtgg tctgcggcat cgcgtctcct agttggcttt
     3841 ttgatgttca tgctagtcat gccaacgcga aaaagcaact gcttggttgg catgagcttg
     3901 ttcacgaaca gatcgacgcg gcgaacgctg cgcgcgaata gtgtttgcga aaaagtgcac
     3961 agtcgtgcta aaaatacgcc tcgcgacagg tgaaccaaat tcaacaaaaa acaggtcata
     4021 ctgcgcgtac atacgaataa cccggcgcaa caacgcacgg aggagacatg gtaggtttgc
     4081 aaatcgcgtt gagcggtatt gcgcagggct gtatctacgg cttgatcgca ctcggcttcg
     4141 tcctgatcta caaggcgacg gagaccgtca acttcgcaca gggcgagttg atgatgctgg
     4201 gggccttcat cggcctgtcc tgcgtcaccc ttctcggcct tccgttctgg ctcgccatcg
     4261 ccgtcaccat gctggccatg ctgtccttcg ggatgatcat ggaacgcagc ctgctgcgtc
     4321 cgctgctcgg ccagcccgcg ttcaccgtcg tgatggtgac catcggcttc ggctatctgg
     4381 cgcgcggcgt cgtgacgatg gtgccgccct ggggcaccga tacccacgtg atgccggcgc
     4441 cttacaagga tgaactggtg cgcatcggcg ccgaaggcgg tcagcagctg gtcgcgtcga
     4501 tggagcagat cgtcatcatc gccgccaccg ccatgctgtg cgcggtcctc ttcctgttct
     4561 tccgcttcac ccggctcggc atcgcaatgc aggccgcctc ccagaaccag ctcgccgcgt
     4621 tctacatggg cattcccgtg cggcgcctga actcgctggt ctggggcatc agcgccgccg
     4681 ttgccgccat cgccggcatc ctgctcgcgc cgatcacctt cgtccattcc aacatgggct
     4741 tcatcggcat gaaggccttc cccgccgcgg tcgtcggcgg gttcggcagc atccccggcg
     4801 cgatcgtcgg cggtctcgtc atcggcctgg tcgagtcctt ttccggctat tacctaccgg
     4861 aagggttcaa ggacgtcgcc gcctacgtgg tcgtcctgat catgctggtg gtgcgcccga
     4921 acggcctgtt cggcgaaaag ctgcgcaagc gcgtataagc cgctgcggcc ccaccctgcc
     4981 gcgactccct acgaaaagaa agaggaaaga agccgatgcg tttcctgttc aagaccgact
     5041 acaaccagga catccgcctg ctcaagcatg agggcatgcg gttctggtat ggcgtgctgg
     5101 cggtactgct gctgctggcg ccctggctgc tgaccgaata ccacctctcg caactggtgt
     5161 tcgtgctgat ctatgcgatt gccgcactcg ggctgatgct gctctccggg ttcaccgggc
     5221 aagtctcgat cggccacgcg gccttcctgg ccgtcggtgc ctacaccgaa gcctacctcg
     5281 ctgcgcgcgg ctggcccttc gtgttctcgc tgaccgccgc cgccggcctg tcggccatca
     5341 ccggcatcat catcggcctg cctgcattgc gcaccaaggg catgtacctg gcgatcgcga
     5401 cgcttgcctt cggcttcatc gtcgaagaaa ttctgacccg cgccgaaggc atcaccggcg
     5461 gcgcaggcgg catgatggtc ggtcgcatcg tcctgttcgg cttcgagatc gacaccgaga
     5521 ccaagttcta cttcctctct ctcgccatta ccgtgatcgt cacgctcgcg gtggtgaacc
     5581 tgctgcgcag cccgaccggc cgcgcgttcg tcgccatccg agactcggaa atctccgcgc
     5641 agagcatggg catcaatctc gcccgctaca agacaacagc gtttgcacta tccgcggcat
     5701 tggcgggcat cgccggcgcg ctgtacgcgc acaagatccg cttcctgacg ccggagcagt
     5761 tcggcttcct gcagtcgatc gaactgctga tgatcatcgt gatcggcggt ctcggcttcg
     5821 tgcacggcgc cttcttcggc gcggcgttct gggtattgat ccagcagctg atcgtcagct
     5881 tcaagggtta tctccccgat gcgatcgcgc accagaccgg gctgcagccg accatcttcg
     5941 gcatcatcct gatcgccttc gtgctgttcg agccgatggg tctgtacggg cgatggctga
     6001 agatacgcac ctacctcgaa ctgttcccct tctatcgcaa gggcatgttc aagcgtcaga
     6061 agtcctacat gaaatcggag cgcctgagat gagcacgccc ctcctccagg cagagaacct
     6121 gaccgtgaag ttcggcgggc tcactgcagt caacaatgtc agcttcactg tgcgcgaagg
     6181 cgagatcttc acgctgatcg gaccgaacgg cgccggcaag acgacgacct tcaacctgat
     6241 cagcggcctg taccagccca ccagcgggac catcaggctg gacggcgaaa cgatttcggg
     6301 cctcgcaccg tatcggatcg ccgaaatggg cgtggcgcgg acgttccaga acatcgagct
     6361 gttcgaacat gcgacggtgc tgcagaacct cctgatcggc cgccatacgc agcgccgaac
     6421 cagcctgtgg caggaaatcg tctttgccga tgcggtacgt cgcgcagagc tggatttccg
     6481 cgccgaagtg gaaaaggtca tcgagtttct cgacctgcag cattaccgtg actcgctggt
     6541 ggccggcctt ccgtatggcg tgcgcaaggt ggtcgaactg gcgcgcgcgc tgtcgatgca
     6601 gccgcgcctg ctgctgctcg acgaaccctc ttccgggctg aacaccgagg aaaccgacga
     6661 catggcgttc tggatcaagg atatccagac ggaccttggc atcacggtga tcatggtcga
     6721 acacgacatg tctctggtca gccgcgtgtc cgaccgcgtg ctcgcgctga actatggcga
     6781 ggtactggca accggcactg ccgccgaggt gcagtcccac cccgccgtca tcgaagccta
     6841 tctgggaggt gtcgaggcat gagcgacatc ctgcaactga acaacgtcga gagcagctac
     6901 ggcccggtga aggcgatccg cggcgtgagc ctgaaggtca aggaaggcag catcgcgacc
     6961 gtgctcggct ccaacggcgc cggcaagtcc acgatcctga aaaccatctc cggcatcatc
     7021 gatccgcaga aaggcacgat cctgttccag ggcaagccga tacagaaaaa ggatccggcg
     7081 gacatcgtgc gcgacggcat cgtccacgtg cccgaaggcc gcgaagtctt cccgctgctg
     7141 tccgtctacg acaaccttct gatgggcagc tacacccgca gcgaccgcga cgcggtcgca
     7201 cgcgacatcg aggccgccta tgcctacttt ccgatcctga aggaacgcgc gaagcaggaa
     7261 gccgggctgc tctcgggcgg ccagcagcag atgctggcga tcgcccgcgg cctgatggcg
     7321 cagccgaagg tgatgctgct cgatgaaccg agccttggcc tgtcgcccaa gctcaccaag
     7381 gaaatcttcg agatcatccg tcgcatcaac gccgagcgcg gcaccacgat cctgctggtc
     7441 gagcagaacg cactggtcgc gctgaactat gcggactacg gttacgtgct ggaagtcggc
     7501 cgcatcgtga tggaagacac ctgtgcggcg ctgcgcgaga aagaggacat caaggagttc
     7561 tatctcggca tgaaggagac cggcgtgcgc ggcgagcggc gctggaagcg caagaagaac
     7621 tggcgataag aggacgcaac gtggacagga tcacgactac agcgatgcag cacacgatcg
     7681 tggacgacac catcatcgtc cccggcgaca acattccccg cctgttctgg aacgcggtgg
     7741 cgaaacggcg cgacaagatc tgcctgcgcc agaaggaata cggaatctgg cagagcatga
     7801 cctacggcga ggtcgcccag gtcgtgagcg agatcggcta tggcctggtc gcgctcggct
     7861 tcgagccggg agagcgcgcg tcgattctcg cgaacacgat acgcgagtgg ttctgcgccg
     7921 acctcgcggt tttgtgcgca ggcggcgtct cgaacggcat ctacccgacc gactccccgt
     7981 cccaggtcga atacctgatg caggattccg gctcggccta catcttcgtc gaggacgagg
     8041 aacagctgga caaggtgctc gaggtacgcg gcagcctgcc cgacctgcgc aagatcatcg
     8101 tgttcgacat ggaaggcctg cgcaacctga acgacgaaca gatcatcagt ctcgatcaac
     8161 tgcgcgcgct cggacgggat tacgaagcgc aggatccgtc ggcgcgcgcg gtcgaatggg
     8221 aaagccggct cgacgcccgc cgccccgagg acctcgcgat cctgatctac acctccggca
     8281 ccaccggacg gccgaagggg gcgatgatct cgcaccacaa tgtgatcacc gcagtgcgcg
     8341 gcttcaacag cgtgctgccc cagcaggagg acgacgagcg catgtgcttc ctgccgctct
     8401 gtcacgtcgc cgagcggctg ggcggcgcct attttgcgct gtactccggc gcgcgcctga
     8461 acttcgtcga gaacccggag acgatcccgg agaacgttcg cgaaatctcg ccgacgatct
     8521 tcctggccgt gccgcgcatc tgggagaagt tctactccgg cgtgatgatc gcgctgtccg
     8581 agggcaccac gctggagaaa ctggcctaca agaaggcgat cgaggtgggc aaggaagccg
     8641 ccaccttcct cg
//
LOCUS       DATLGF010000108         2085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_145514, whole genome shotgun sequence.
ACCESSION   DATLGF010000108 DATLGF010000000
VERSION     DATLGF010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145514"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="VM406_03215"
     CDS             complement(<1..161)
                     /locus_tag="VM406_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HVL75000.1"
                     /translation="MSASSSPSPLLAGFPPVLDADTRVLILGSFPGVASLAAQQYYAH
                     PRNQFWRLVG"
     gene            297..1052
                     /locus_tag="VM406_03220"
     CDS             297..1052
                     /locus_tag="VM406_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL75001.1"
                     /translation="MHPDKGLVLITGGSRGIGAATAVLAAERGHAVCISYLQNRAAAD
                     EVADRIRGAGGSALAVRADVASEEDVMRMFDAVDASGHRLAGLVNNAGILERQMRVED
                     MDAARITRVLATNVTGSFICAREAVRRMSTRHGGRGGAIVNVSSMAARLGSPNEYVDY
                     AASKGAIDAMTVGLAREVAAEGIRVNAVRPGLIATDIHASGGEPGRVERVKGSVPMRR
                     GGEAIEVARAILWLLSDEASYSTGTFIDVSGGR"
     gene            1072..1839
                     /locus_tag="VM406_03225"
     CDS             1072..1839
                     /locus_tag="VM406_03225"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVL75002.1"
                     /translation="MYLWHGRALIVGPGIDSSPHAHHAAQLTLALERPVRVRRDATQP
                     WQETRAALFAPNDAHQLDCGGAPMAHLFIDMPLRPAALPSELRASFADDPVFEPVRMA
                     LDKAAHTGLDESAAARLVREWLECALPGAPAGFGYDPRIGQALQWIARHPGERTDGRA
                     LAAMVHLSESRFTHLFRQQTGLSLSRYLIWARLLHAVELAADGASMTEAAHGAGFADL
                     AHLSRSFRGTFGVMPSALRKMTIVFKRADNAATTLAP"
     gene            1869..>2085
                     /locus_tag="VM406_03230"
     CDS             1869..>2085
                     /locus_tag="VM406_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HVL75003.1"
                     /translation="MENLASRQIETLLARYAESHRHPQNEVIHFICIPAIVFSLLGLL
                     WVAHPLVAVVVTAASLIYYLTLSVPFAL"
BASE COUNT          327 a          683 c          748 g          327 t
ORIGIN      
        1 ccgaccaggc gccagaactg gttgcgcgga tgcgcgtagt actgctgcgc ggcgagcgag
       61 gcgacgccgg gaaagctgcc gaggatcagg acgcgggtgt cggcatcgag gaccggcgga
      121 aagccggcga ggagcgggga gggggagctg ctggcggaca ttcgggcatt ctagccgggt
      181 gtcggtgttg cttctggagg tggtgtttgc ggcaatgcgg gaggaaacgc ggcatgcccg
      241 attcgcaagc acgaaactgc gtaatatcct gcaaacgagt attccacgga gacgccatgc
      301 acccagacaa aggcctcgtc ctgatcaccg gcggcagccg cggcatcggc gccgccaccg
      361 ccgtgcttgc ggccgagcgc ggccatgccg tgtgcatcag ctacctgcag aaccgcgcgg
      421 cggccgacga ggtggccgac cgcatacgcg gcgccggcgg cagcgcgttg gcggtgcgcg
      481 ccgatgtggc ttccgaagag gacgtgatgc gcatgttcga tgcggtcgac gcaagcggcc
      541 accggctcgc aggcctggtc aacaatgccg gcatcctcga gcgccagatg cgcgtggaag
      601 acatggatgc ggcgcgcatc acgcgcgtgc tggcgaccaa cgtcaccggc agcttcatct
      661 gcgcgcgcga ggcggtgcgc cgcatgtcga cgcgccatgg cgggcgaggc ggcgcgatcg
      721 tgaatgtctc gtcgatggcg gcgcgcctgg gttcgccgaa cgagtacgtc gactatgccg
      781 cgtccaaggg cgcgatcgac gcgatgacgg tgggcctggc gcgcgaagtg gccgccgaag
      841 gcatccgcgt caatgcggtg cgcccgggac tgatcgcgac cgacattcac gccagcggcg
      901 gcgagcccgg acgggtggag cgcgtgaaag gcagtgtgcc gatgcggcgc ggcggcgaag
      961 cgatcgaagt cgcgcgcgcg atcctgtggc tgctttcgga cgaggcgtcc tacagcaccg
     1021 ggaccttcat cgacgtcagc ggcggccgct gacatgcacg ctcacacctc catgtatctc
     1081 tggcacgggc gcgcgctgat cgtcggcccg ggcatcgatt ccagccccca tgcccatcac
     1141 gccgcccagc tcacgctggc gctcgagcgg ccggtgcggg tgcggcgtga tgcgacccag
     1201 ccgtggcagg aaacccgggc cgcgctgttc gcgccgaacg atgcgcatca gctcgattgc
     1261 ggtggtgcgc cgatggctca tctgttcatc gacatgccgc tgcgcccggc cgcgctgcct
     1321 agcgagctgc gggcatcgtt cgccgatgat ccagtcttcg agccggtgcg catggcgctc
     1381 gataaggccg cgcacaccgg gctcgacgaa agcgccgccg cgcgtctcgt acgggagtgg
     1441 ctcgaatgtg cgctgccggg cgcgcccgcc ggcttcggct acgatccgcg catcggccag
     1501 gcgctgcagt ggatcgcccg ccatccgggc gagcggacgg acggccgcgc gctcgcggcc
     1561 atggtgcacc tgtcggaaag ccgtttcacc cacctgttcc ggcagcagac cggcctgtcg
     1621 ctttcacgct acctgatctg ggcgcggctg ctgcatgcgg tggaactggc ggcggacggc
     1681 gcgagcatga ccgaggcggc ccatggggcc ggttttgccg acctggccca cctgagccgg
     1741 tctttccggg gcaccttcgg cgtgatgccg tcggcgctgc ggaaaatgac gattgtgttc
     1801 aagcgcgccg ataacgcggc gactacactg gcgccatagg gctcgcgtcg aagcctgagg
     1861 agactgagat ggaaaacctt gcaagccgtc aaatcgaaac cctgctcgcg cgctatgcgg
     1921 agagccaccg ccatccgcag aatgaagtga tccacttcat ctgcatcccc gcgatcgtgt
     1981 tctcgctgct gggcctgctg tgggtcgcgc atccgctggt cgcggtagtc gtgaccgcgg
     2041 cctcgctgat ctactacctg acgctgtcgg tgccgttcgc gctcg
//
LOCUS       DATLGF010000109         2801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_66341,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000109 DATLGF010000000
VERSION     DATLGF010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66341"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VM406_03235"
     CDS             complement(<1..495)
                     /locus_tag="VM406_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HVL75004.1"
                     /translation="MSASTAGLELRTERRWLAEAVELVSSMRFAISLLTLISIASVIG
                     TVLQQNEPMPNYVNQFGPFWFEVFDKLGLYAVYSAWWFLLIMTFLVISTTLCIVRNAP
                     KMLKDMRSWRENVREQSLRNFPHRAEWSGSGAPEQFAGVIARRVEDSGYKLKMFAKDG
                     GVLIA"
     gene            complement(616..1266)
                     /locus_tag="VM406_03240"
     CDS             complement(616..1266)
                     /locus_tag="VM406_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVL75005.1"
                     /translation="MKRAFLPLVKSLFVAVIGVASIAHAQEAVKVDPARGEKIYSSGD
                     AARNVTACVACHGAAGNSTIAQNPKLAAQHAPYLVKQMVDFQSPQRANSVMSPIAKAM
                     SEQDIRDVAAYLEIQEKKPGAARNKDTIELGKQIYRGGIPEKNVPACAACHGPSGSGM
                     PALYARLGGQHQDYTAQQLINFRARARNNSEQMVTIARRMTDDEIQAVADYIAGLR"
     gene            1481..2164
                     /gene="yihA"
                     /locus_tag="VM406_03245"
     CDS             1481..2164
                     /gene="yihA"
                     /locus_tag="VM406_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HVL75006.1"
                     /translation="MSLLWQARFLTTVNHLGDLPRLPVPEVAFAGRSNAGKSSAINVL
                     CNQKRLAFASRTPGRTQHINYFSIGGAHVAQHRNDPTRLEEVRALLVDLPGYGYAEVP
                     GEAKRHWNELLGTYVQTREQLCALVLIMDARRPFTELDVQMLEWFASTGKPVHCLLTK
                     ADKLNRSESTNALRLARTVLASYVDAQGRPFPFTAQLFSAMKRIGLEEAEQKVLDFLG
                     LGQGEETSE"
     gene            2373..>2801
                     /locus_tag="VM406_03250"
     CDS             2373..>2801
                     /locus_tag="VM406_03250"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139692.1"
                     /note="catalyzes the formation of porphobilinogen from
                     5-aminolevulinate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HVL75007.1"
                     /translation="MTASLPPAAFPAVRMRRMRKDAFSRAMIREHVLTPADLIYPVFI
                     LDGKNQREKVASMPGVERLSADLLLAVAEDCVALGIPVLALFPVINPALKTPDGSEAL
                     NPDGLVPRAVRELKSRFPELGLLTDVALDPYTSHGQDGVIN"
BASE COUNT          533 a          921 c          813 g          534 t
ORIGIN      
        1 agcaatcaac acgccaccgt ccttggcgaa catcttgagc ttgtagccgc tgtcctcgac
       61 gcgccgggcg atgacgcctg caaactgctc cggcgcgccg ctgcccgacc attccgcccg
      121 atgcgggaag ttgcgcagcg attgttcgcg cacgttctcg cgccagctgc gcatgtcctt
      181 gagcatcttc ggggcattgc gcacgatgca cagcgtggtc gagatcacca ggaaggtcat
      241 gatgagcaag aaccaccatg cagagtacac cgcgtacagc ccgagcttgt cgaacacctc
      301 aaaccagaag gggccgaact ggttgacgta gttcggcatc ggctcgttct gctgcagcac
      361 cgtgccgatc accgatgcaa tcgaaatcag cgtcagcaga ctgatcgcga aacgcatcga
      421 tgacaccagc tcgaccgcct ctgccagcca gcgccgttcg gtcctcagtt ccaggcccgc
      481 tgtacttgca ctcatcacac tttactcaca cgacccacgc acctggcgcg tagtcattca
      541 tgcagcttgc gccgctcaaa aaataagggg cggcttgcgc cacccctgcg tccttccctg
      601 ttcttgttgt cttttttagc gcaggccggc gatgtaatct gccactgcct gaatctcgtc
      661 gtccgtcatg cggcgcgcga tggtcaccat ctgttcgctg ttgttccggg cacgcgcacg
      721 gaaattgatc agctgctgcg cagtatagtc ctgatgctgg ccgcctaggc gcgcatacag
      781 cgccggcatg ccggaaccgc tgggaccatg gcaggcggcg caggccggca cgttcttctc
      841 gggaatgccg ccgcgataga tctgcttgcc gagctcgatg gtgtccttgt tgcgcgcggc
      901 gcccggcttc ttttcctgaa tctcgagata agccgccacg tcacggatgt cctgttcgct
      961 catggccttg gcgatcggcg acatgacaga attggcgcgc tggggcgact ggaagtccac
     1021 catctgcttg accagatacg gcgcatgctg ggctgccagc ttcgggttct gggcaattgt
     1081 cgaattgccg gccgcgccat ggcaggccac gcaagcggtg acgttgcgtg ctgcatcgcc
     1141 gctgctgtag atcttctcgc cgcgggccgg gtcgaccttg acggcttcct gcgcatgggc
     1201 aatcgaagcc acgccgatca cggcgacaaa cagggatttc accaacggta ggaatgcacg
     1261 cttcattcaa acacctgaga caaagtatta agtattcttt tcggccgaca catgccgcgc
     1321 cggcttggcg ctactgcaaa taacctctta ttgtacaata ggcgggctca atgttgcgag
     1381 gaaagtcgac gcgccgcgcc ctgccgcccc gtcctcgcgc ccctcgaata cgcctttcgg
     1441 tccgctcgtt cgccggactg cgccaacccc tcttcacctc atgtccctgc tctggcaagc
     1501 ccgttttctc acgaccgtca atcacctggg cgacctgccg cgcctgccag tccccgaagt
     1561 cgcgttcgcc ggtcgctcga acgccggcaa atcgagcgct atcaacgtgc tgtgcaacca
     1621 gaagcgcctt gcgttcgcct cgcgcacacc gggacgcacc cagcacatca actatttctc
     1681 gatcggcggc gcgcacgtcg cccagcaccg caatgaccca acccgcctgg aggaagtgcg
     1741 cgcgctgctg gtggacctgc ccggatacgg ttacgccgaa gtaccgggcg aagccaagcg
     1801 ccattggaat gaattgctcg gcacctatgt gcagacccgc gaacagcttt gcgcgctggt
     1861 gctcatcatg gacgcccgcc gtccgttcac cgaactcgac gtgcagatgc tcgagtggtt
     1921 cgcctccacc ggcaaaccgg tgcattgcct gctgaccaag gcagacaagc tcaaccgcag
     1981 cgaatcaacc aatgcgctgc gcctggcgcg cactgtgctg gccagctatg tcgacgcaca
     2041 gggcagaccc tttccgttca ccgcgcagct gttttcggcg atgaaacgca tcggccttga
     2101 ggaagcggag cagaaggtgc tggactttct cggactcggc cagggcgaag agacatccga
     2161 ataagacggg caaaaaaaac cctggagaaa ggggaagatc tccagggtag taacgccgta
     2221 ccgcgtctgc ggccggcccc gctcagggag gagaagcggg aggtgtgtga acacctgttg
     2281 ctaagagggc cagcggccga aaaagtttcg cttcccgtag aatttgatcg attttgactc
     2341 ttcgctcgag acgagcgcaa aggaccgata ccatgacagc ctcccttccg ccggccgcct
     2401 ttcccgccgt ccgcatgcgg cgcatgcgca aggacgcatt ttcgcgcgca atgatccgcg
     2461 agcacgtcct cacgccggcc gacctgatct atccggtttt catcctggac ggcaagaacc
     2521 agcgcgagaa ggtcgcctcg atgcccggag tggaacgcct gtcggcggac ctgctgcttg
     2581 cagtggcgga agactgcgtc gccctcggca ttccggtgct ggcgcttttt ccggtaatca
     2641 atcctgcgct gaagacgcca gacggcagcg aagccttgaa tccggacgga ctggtcccgc
     2701 gggcggtgcg cgaactgaag tcgcgcttcc cggaactggg gctgctgacc gacgttgcgc
     2761 tcgaccccta caccagccac ggccaggacg gcgtcatcaa c
//
LOCUS       DATLGF010000110         2666 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_208610, whole genome shotgun sequence.
ACCESSION   DATLGF010000110 DATLGF010000000
VERSION     DATLGF010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208610"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VM406_03255"
     CDS             complement(<1..629)
                     /locus_tag="VM406_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVL75008.1"
                     /translation="MDIKHYMNEVGQRARKASRAMAKADTAAKNRALMLIAAAIRRDA
                     DALRAANRKDLDAARANGLADAMLDRLTLSDKAIATMAEGLEQIAALPDPIGEISNMK
                     YRPTGIQVGQMRVPLGVIGIIYEARPNVTVDAAGLCIKSGNATILRGGSEAIHCNQAL
                     AALVKEGLDGAGLPQDAVQIIETTDRAAVVSMICTASCGRPAPSRPSFT"
     gene            complement(756..1772)
                     /gene="holA"
                     /locus_tag="VM406_03260"
     CDS             complement(756..1772)
                     /gene="holA"
                     /locus_tag="VM406_03260"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HVL75009.1"
                     /translation="MQLRPDALDAQLAKSLAPLYVITSDEHLLALEAADKIRKAARAQ
                     GFTERDVLVVERYFKWGALTEANQSQSLFGDRKLIELRIPSGKPGKEGGQALQDYVAN
                     LNPDNVTLISLPKLDWQTQKSAWVAALQRAAAYIEIPLVERPQLPAWVGSRLAAQGQS
                     AERASLDFIADRVEGNLLAAHQEIQKLALLHPPGKLSFEQVQDAVLNVARYDVFKLNE
                     AMLAGDAVRLTRMIEGLKGEGEALPLVLWAMAEEIRTLLRLKSGVAQGKALGVLMKES
                     RIWGPRERLMEPALRRLSLPQLESALREAAQVDRMVKGLRAKAFAGDAWDALLQLGLK
                     VARG"
     gene            complement(1775..2263)
                     /gene="lptE"
                     /locus_tag="VM406_03265"
     CDS             complement(1775..2263)
                     /gene="lptE"
                     /locus_tag="VM406_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HVL75010.1"
                     /translation="MKRRTALLALSTLGLSACGFQLRGSGRQSALRFKTLHIAGNNGG
                     LAFALRRMIEAGNGTTVVDDPKQAEAIIDILGEARDRGTITLNTQGRIREYSLYYRVN
                     FRVRGAAGQEMLAPTLIVLRRDLSFNEAQAIAKEREEEMLFRDMQGDAVQQILRRLAA
                     IH"
     gene            complement(2268..>2666)
                     /locus_tag="VM406_03270"
     CDS             complement(2268..>2666)
                     /locus_tag="VM406_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HVL75011.1"
                     /translation="KMLNTLEGAKLSDSAQTNAVIAEGLSIFLRVLYPVAPHIAHVLW
                     NELGFEVAHGDILDAPWPQVDPAALEQAEIELMVQVNGKLRGSVIVPKDADKAAIEAA
                     ALANENVKKFVEGAPKKVIVVPGKLINIVA"
BASE COUNT          406 a          915 c          852 g          493 t
ORIGIN      
        1 ctggtgaagg aaggcctgga tggcgccggc ctgccgcagg acgcggtgca gatcatcgag
       61 acgaccgccg cgcggtcggt cgtctcgatg atctgcaccg cgtcctgcgg caggccggcg
      121 ccatccaggc cttccttcac cagggccgcg agcgcctggt tgcagtggat cgcttccgag
      181 ccgccgcgca ggatggtcgc gttgccgctc ttgatgcaca ggccggcggc gtcgacggtc
      241 acgttcggac gcgcctcgta gatgatgccg atcacgccca gcggcacgcg catctgcccg
      301 acctggatgc cggtcgggcg gtacttcatg ttcgaaattt cgccgatcgg gtccggcagg
      361 gcggcaatct gttccaggcc ttcggccatt gtggcaatgg ccttgtcgga cagcgtcagc
      421 cggtccagca tcgcatcggc caggccgttc gcgcgcgccg cgtccagatc cttccggttg
      481 gctgcgcgca aggcatcggc atcgcgccgg attgccgccg cgatcagcat cagcgcacgg
      541 ttcttcgcgg ccgtgtcggc tttcgccatc gcccgcgagg ccttgcgcgc ccgctggccg
      601 acttcgttca tgtagtgctt gatgtccatt gttccgctca caaaaaataa tccgctgatg
      661 ttgcaggcgc gggcaggttg cgccgacgcg ggaagggggc gttccggcga aagccggatc
      721 cggcggcgct cgcccggaaa acggccaatc cccgtttatc cgcgcgcgac cttcaaaccc
      781 agttgcagca acgcgtccca cgcatcgccg gcaaaggcct ttgcgcgcaa gcccttcacc
      841 atcctgtcca cctgggccgc ctcgcgcagc gcgctttcca gctgcggcag cgagagccgc
      901 cgcagcgccg gctccatcaa ccgctcgcgc ggaccccaga tccgggattc cttcatcagc
      961 acgcccagcg ccttgccctg ggcgacgccg gacttgagcc tgagcagcgt gcggatttcc
     1021 tcggccatcg cccacagcac cagcggcagc gcctcgcctt cgcccttcaa gccttcgatc
     1081 atgcgcgtaa gccgcaccgc atcgccggcc agcatcgctt cgttcaattt gaacacgtca
     1141 tagcgcgcca cgttcagcac cgcatcctgg acctgctcga aactcagctt gccgggtgga
     1201 tgcagcagcg cgagcttctg gatttcctgg tgcgcggcga gcaggttgcc ctcgacgcgg
     1261 tcggcgatga aatccaggct cgcgcgttcc gcgctctggc cctgggcggc aaggcggctg
     1321 ccgacccagg caggcagctg cggccgctcg accagcggga tctcgatgta ggcggcggcg
     1381 cgctgcagtg ccgccaccca ggcgctcttc tgcgtttgcc agtccagttt tggcaggctg
     1441 atcagcgtga cattgtccgg attcagattg gccacgtagt cctgcagcgc ctgcccgcct
     1501 tccttgcccg gcttgccgga aggaatacgc agttcgatca gcttgcggtc gccgaacagc
     1561 gactgcgact gattcgcttc ggtaagcgct ccccacttga agtaacgctc gactaccagc
     1621 acgtcgcgtt cggtgaagcc ttgtgcccgg gccgccttgc ggatcttgtc ggccgcctcc
     1681 agcgcgagca ggtgctcgtc gctggtgatc acgtacagcg gcgccagcga cttcgcaagc
     1741 tgtgcgtcga gtgcgtcggg ccgcagctgc atggctagtg gatcgcggcc agacgccgca
     1801 gtatctgctg gaccgcgtca ccctgcatgt cgcgaaacag catttcttcc tcgcgttcct
     1861 tcgcgatcgc ctgcgcttcg ttgaagctca ggtcgcgccg caggacgatc agggtcggcg
     1921 ccagcatttc ctggccggcg gcgccgcgca cccggaagtt cacgcggtag tacagcgagt
     1981 actcgcgaat gcgtccctgg gtgttcagcg tgatcgttcc gcggtcacgg gcttcgccga
     2041 ggatgtcgat gatcgcttcg gcctgcttcg gatcgtcgac cacggtcgtg ccgttgccgg
     2101 cctctatcat gcggcgcagc gcaaaggcga gaccgccatt gttgccggca atgtgcaggg
     2161 tcttgaaccg cagggccgac tgccggcccg agccgcgcag ctggaaaccg caggcggaca
     2221 ggcccagggt ggacaaggcg agcagggcgg tgcgacgttt catgggatta cgcgacgatg
     2281 ttgatcaact tgccgggaac gacgatcacc ttcttcggcg cgccttcgac gaacttcttc
     2341 acattctcgt tggccagcgc cgccgcttcg atcgcggcct tgtcggcgtc cttgggcacg
     2401 atcaccgagc cgcgcagctt gccattgacc tgcaccatca gctcgatctc ggcctgttcg
     2461 agcgcggcgg gatcgacctg cggccagggc gcatcgagga tgtcgccatg cgcgacctcg
     2521 aagccgagct cgttccacag cacatgcgcg atatgcggcg cgaccgggta gagcacgcgc
     2581 aggaagatcg acaggccctc cgcaatcacc gcgttggtct gggccgagtc gctcagcttc
     2641 gcgccttcca gcgtgttgag catctt
//
LOCUS       DATLGF010000111        16027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_145646, whole genome shotgun sequence.
ACCESSION   DATLGF010000111 DATLGF010000000
VERSION     DATLGF010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 16027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16027
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145646"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VM406_03275"
     CDS             complement(<1..583)
                     /locus_tag="VM406_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HVL75012.1"
                     /translation="MSLSSRSHAETGTSSAGPLAGIRILDMATVLAAPFGTTLCADMG
                     ADVIKLELPDGSDALRTLAPIDLFWKVTNRGKKGITLDVRTPEGRELFLKLAATVDVL
                     VENFRTGTLDKWGLDLATLHAANPRLIVLRLTGFGQTGPYAGRPGFARIFEAMSGFAN
                     LTGDAQSGPQHMNFPLGDVVSGVFCAFSIASAVA"
     gene            complement(700..1980)
                     /gene="cls"
                     /locus_tag="VM406_03280"
     CDS             complement(700..1980)
                     /gene="cls"
                     /locus_tag="VM406_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HVL75013.1"
                     /translation="MPRSSHIRTIVWTAIVTVILALVVTNLTTADPRLRTQVEHRYGV
                     AEPQFLRTMGVLLGPPVAEGNSVQHLENGVRIFPAMLEAIASAERTITFEMYIYWSGE
                     IADRFAAALIERARSGVKVHVLLDWVGSAKMDLTLIDRMRDGGIEVERFHKPTIRNLP
                     RMNNRTHRRILVIDGKVGFTGGVGIGDEWTGDAHGPDHWRESHFRVEGPVVAQMQAVF
                     VDNWTRATGRVLYTEDYFPSLTARGQAPAQMFSSSPAGGSESMHLMYLLAISAAQESI
                     LLSGSYFVPDALAVEALVKARERGVRVSIIVPGPHMDVEVVRRASRARWGELLAAGVE
                     IAEYQPTMYHVKVLVIDGFMVSVGSTNFDNRSFRINDEASLNVYDRQLAAQMQQVFEA
                     DLAKSKRVTLEMWENRPFREKVVEQVASQLGRQL"
     gene            complement(2101..2460)
                     /locus_tag="VM406_03285"
     CDS             complement(2101..2460)
                     /locus_tag="VM406_03285"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HVL75014.1"
                     /translation="MQIQLNTDKHVVGSPSLQERVEQMLEQELKHLSDRITRIEVHLN
                     DVNSAKAGDDDKRCQLEARLAGANPISVEHRAPNMELAIAGAAEQLARALANLQGKAE
                     ASSKRRESIRHMDPGGL"
     gene            complement(2778..4289)
                     /gene="ntrC"
                     /locus_tag="VM406_03290"
     CDS             complement(2778..4289)
                     /gene="ntrC"
                     /locus_tag="VM406_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HVL75015.1"
                     /translation="MKPIWIVDDDESIRWVLEKALARENLATRSFSNVRDAMAALQDG
                     APQVLVSDIRMPGESGLALLQAAKEMHPGLPVIVITAFSDLDSAVAAFQGGAFEYLAK
                     PFDVDKAVELIRRALEESLREADVEQANGVTPEILGQAPAMQDVFRAIGRLSQSNVTV
                     LITGESGTGKELVARALHKHSPRAAQPYIALNTAAIPKDLLESELFGHERGAFTGAQA
                     MRRGRFEQAENGTLFLDEIGDMPFDLQTRLLRVLSDGHFYRVGGHQPLKANVRVIAAT
                     HQNLEQRVRDGLFREDLYHRLNVIRLRLPSLRERREDIPLLTRYFLAQSARQLGVEAK
                     RMSDAAMHYLTGLDFPGNVRQLENLCNWITVMAPGQTVEIKDLPPDLVRGETTGSTQS
                     ATGPDGSQAPAPQEESPVPVPTIEAAREPGLAPAVRPGWISLLEAEAAQMLAAGQPEV
                     MDALGRQFESALIKTALRHTHGRRNDAAVRLGIGRNTITRKIQELGIDGAADD"
     gene            complement(4323..5396)
                     /gene="glnL"
                     /locus_tag="VM406_03295"
     CDS             complement(4323..5396)
                     /gene="glnL"
                     /locus_tag="VM406_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HVL75016.1"
                     /translation="MKHSPPSLEGLELLSSSVLLLDAEDRVVYANAAAENLLESSFRM
                     LAQQRIADLFFNGGQLHPVLEQARAHEFADKRLDLALERAGREPLHVHLIVTALDIPA
                     TPVLLELRENVQQLKLDREERLLDQSQLNKELIRNLAHEIKNPLGGIRGAAQLLELEL
                     PERHLRELREYTQVIVKEADRLQTLVDRLLAPHRHPHIVGDVNIHEVCERVRSLMLAE
                     FPSGLTITRDYDLSVPEFRGDKEQLIQAVLNIVRNAAQALEEKIADGDAQITLRTRVA
                     RQITLAKIRYRLALDLHIVDNGPGIPPDIQDRVFYPLVSGRAGGSGLGLTLAQTFVQQ
                     HMGVIECESRPGCTDFRVLIPLP"
     gene            complement(5585..6094)
                     /locus_tag="VM406_03300"
     CDS             complement(5585..6094)
                     /locus_tag="VM406_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HVL75017.1"
                     /translation="MKKQLSLLLLLSGFAGVAHAQGEVYVCVDEDGRREYRNTGMTKG
                     CKRVDLPGLSMVPAPASAPAPARAAPAAPTAAARPAAAPVDFPKVDSATQRARDTDRR
                     RILQDEMRAEEQKLAGLKKEFNNGEPERRGDERNYAKYQERVALMQEDIRRTEKNIEA
                     LQRELNNLR"
     gene            complement(6289..7704)
                     /gene="glnA"
                     /locus_tag="VM406_03305"
     CDS             complement(6289..7704)
                     /gene="glnA"
                     /locus_tag="VM406_03305"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HVL75018.1"
                     /translation="MARTAEEVLKMAKENEVKFIDFRFADTRGKEQHVTVPVSQFGMD
                     KFESGHAFDGSSIAGWKGIEASDMLLMPDPNTANIDPFMEETTLFMQCDVIEPSDGKG
                     YDRDPRSIAKRAEAYLKSSGIGDTAYFGPEPEFFIFDGVRWKIDMSGCFVKVDSEEAS
                     WSTDREFEGGNTGHRPTVKGGYFPVPPVDSFQDMRSEMCLILEALGIPVEVHHHEVAG
                     AGQNEIGTKFSTLVERADWTQTLKYVIWNVAHTYGKTATFMPKPIVGDNGSGMHVHQS
                     VWKDGKNLFAGDGYAGLSDFALYYIGGIIKHARALNAITNPGTNSYKRLVPGFEAPVK
                     LAYSARNRSASIRIPHVANPKARRVEARFPDPLSNPYLCFSALLMAGLDGVQNKIHPG
                     EAASKDLYHLPPEEDALIPTVCSSLDQALEYLDKDREFLTRGGVFSDTMIDAYIELKM
                     QEVQRFRMTTHPVEFDMYYSL"
     gene            8045..8503
                     /locus_tag="VM406_03310"
     CDS             8045..8503
                     /locus_tag="VM406_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HVL75019.1"
                     /translation="MSLNILETARERGRQHNLPYAGALTPQEAYALLQQEKSAMLVDV
                     RTNAERDWVGRVKIDPAQHAAIEWSLYPGGTPNPAFLDQLQSAAQKDDVLLFLCRSGV
                     RSRHAARLATEHGFLNCFDILEGFEGDKDANGHRKTVCGWCKAGLPWVGA"
     gene            complement(8516..9862)
                     /locus_tag="VM406_03315"
     CDS             complement(8516..9862)
                     /locus_tag="VM406_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HVL75020.1"
                     /translation="MSRIFSFSRISLRGLLFSAGPVLMLLAAACLLAYWIVDPSPPPK
                     VTLSTGQENSAYEEIGKRYAAQLARMGVKVDLVRSLGSQENLDRLVDPRSGVGIAFVQ
                     SGSTEMAQAERQGLVSLGSLFTEPVWLFMRERKAVTRLSQLRGMKVNVGPEGTGVPQL
                     FKRILAVNGIEPGELALSGLENTPATVALLEGQIDALVFSSAPDAPLVQMLLQTPGIR
                     LFDFAQAEAYTRRLPFLSHVVLPRGIVDLGRDLPARDYHLIAPTATLVARADLHPALV
                     DLFVHAAAQIHNDAGWFQRRGEFPNANYTEIPVASSAQKYYRSGPPFLQRYLPFWLAN
                     LFDRMWVIVVALGALMLPLSRIVPPLYVWQVRSRVYRWYGQLRAVEQALEEVPQDRRA
                     DVYREQLERLNEIEDRVNHIAIPLSFADELYGLRSHIGFVRRRIAALMEERAGEPA"
     gene            complement(9962..10525)
                     /locus_tag="VM406_03320"
     CDS             complement(9962..10525)
                     /locus_tag="VM406_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75021.1"
                     /translation="MLTATYSLVAIAAEQEKTRTMLNRLQQFLDAAWSGLQNVDFGFL
                     ESGYHRLLQFDQYLRERKIERFLIPVLRGTGREAELLIAEMESLRHKSIGYLREAGAL
                     LASRTDFGRLQVSRVCESMSAYCSQAASRLTLEEQSLLPLARRLLSVEDWFRLASDFL
                     SRDSAPGRRSGRPNRGYAHPGARRDMH"
     gene            complement(10671..12329)
                     /locus_tag="VM406_03325"
     CDS             complement(10671..12329)
                     /locus_tag="VM406_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HVL75022.1"
                     /translation="MASITETPDTVDAASAPRKPAELIAREVARRRTFGIISHPDAGK
                     TTLTEKLLLFSGAIQLAGTVKARKSGRHATSDWMEIEKQRGISVASSVMQFEYREHVV
                     NLLDTPGHEDFSEDTYRVLTAVDSALMVIDAAKGVEEQTIKLLNVCRMRATPILTFMN
                     KLDRETRDPLELLDEVESVLKIQCAPVTWPIGMGKNFRGVYHLLRDEILLFKAGEERA
                     DQEFEVVKGIDNPRLAELFPMEMEQLKMEVELVHGASHPFDLDAFLAGTQTPVFFGSA
                     INNFGVREILNALLDWAPAPRSRDATVRQVEPVEEPFSGFVFKIQANMDPAHRDRIAF
                     LRVCSGRFERGMKVRHLRLNREIKVSSVVTFMASSREQVEEAYAGDIIGLPNHGNMQI
                     GDSFSEGEMLQFTGIPYFAPDFFRLVRIRNPLKIKQLHKGLQQLGEEGAVQVFKPVHG
                     GDLILGAVGVLQFEVVASRLMNEYGVDAVFEGTSISSARWISSDDKKALADFERSSAG
                     ANLAHDAAGNLAYLATSGVNLRLTQERWPQLTFHSTREHAARLE"
     gene            complement(12413..13000)
                     /gene="orn"
                     /locus_tag="VM406_03330"
     CDS             complement(12413..13000)
                     /gene="orn"
                     /locus_tag="VM406_03330"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HVL75023.1"
                     /translation="MSQATPSERPAAAPARPNEFNLVWVDMEMTGLDPDTDRIIEVAV
                     VVTDSELNVLAEGPVFAIHQPDEVLDGMDAWNKGTHGRSGLIDRVKASKVSEADAEAA
                     LIDFLKHFVPAGKSPMCGNTICQDRRFMARGMPKLEAFFHYRNLDVSTLKELCKRWKP
                     EVASGFKKHQKHTALADILESIEELRYYREHFIQR"
     gene            13042..14304
                     /locus_tag="VM406_03335"
     CDS             13042..14304
                     /locus_tag="VM406_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HVL75024.1"
                     /translation="MTSLAFSILFVGFLLLTLSLRFWLASRHIRHVQRHRDAVPAEFS
                     HKIPLYAHQKAADYTVARTRFGLVTMIVNAAVLIGFTLFGGLQWLSVAVFDATGPGMW
                     HQLGLVAAFAVISGLVDLPFDYYRQFGLEERFGFNKMTPKLFFSDLVKSVLLGVAIGL
                     PLLWVILMLMEQAGDLWWFYAWLVWIAFQLLMLVLYPTVIAPMFNKFTPLEDASLRER
                     IEGLMKRVGFASKGLFVMDGSKRSAHGNAYFSGFGAAKRIVFFDTLLARLAPQEIEAV
                     LAHELGHFKLRHIIKRIAVMFGLSLAFLALLGYLKNQVWFYTGLGVDPMVPGNNDAMA
                     LILFMLVLPVFTFVFAPLASLTSRKHEFEADAFAARHTDPQDLVSALVKLYEDNASTL
                     TPDPIHSAFYDSHPPASVRIQHLLAQQH"
     gene            14304..15197
                     /gene="rsgA"
                     /locus_tag="VM406_03340"
     CDS             14304..15197
                     /gene="rsgA"
                     /locus_tag="VM406_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HVL75025.1"
                     /translation="MEPMRGTVIAAHGRHYLVQAGEQRLNCVTRGKKSDVATGDMVEI
                     KQTSQDQGVIEKIGERRTLLYRSDQYKSKLLAANVTQLFIVVATEPGYSDDLVSRALV
                     AAEAAGVAPHLILNKIDVTASLDKTRERLRMYAAIGYPIHEVSVRTQPEQTRALLMPM
                     LQGHATILIGQSGMGKSSLINLLVPDADLATREISDALDSGKHTTTFTRLFALDAHTA
                     VIDSPGFQEFGLYHLTEGMLERAFPEFAPHLGQCRFYNCHHLSEPGCAVLGAVETGAI
                     HPMRHALYQQLVHESSQKIGY"
     gene            15306..>16027
                     /locus_tag="VM406_03345"
     CDS             15306..>16027
                     /locus_tag="VM406_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HVL75026.1"
                     /translation="MAIKHFLQVSDFTLDEFEHVIERASVIKRKFKNYEPYHPLVDRT
                     LVMVFEKNSTRTRLSFEAGMHQLGGAAIYLNTRDSQLGRGEPVEDAAQVMSRMCDIIM
                     IRTFGQDIIERFAAHSRVPVINGLTNEHHPCQVLADIFTYIEHRGSIAGKTVAWIGDA
                     NNMLYSWLQAAQVFGFHVNVSTPKRYEIDPALVAPDNSSYTVFADPSDACENADLVTT
                     DVWTSMGYEEENAARLKAFKGW"
BASE COUNT         2811 a         5144 c         5092 g         2980 t
ORIGIN      
        1 gcgcgacggc gctggcgatc gagaacgcac agaaaacacc cgagaccacg tcgcccaacg
       61 ggaaattcat gtgctgcggt ccgctctgcg cgtcgccggt gaggttcgcg aagccgctca
      121 tcgcctcgaa gatgcgcgca aagcccgggc gtccggcata cggcccggtc tggccgaatc
      181 cggtcaggcg gagcacgatc aggcgcggat tggctgcatg cagggtggcg aggtccagac
      241 cccacttgtc cagcgtgccg gtgcggaaat tctcgacgag cacatcgacg gtggccgcca
      301 gcttcaggaa cagctctctc ccttccggtg tgcgcacatc gagcgtgatg cccttcttgc
      361 cgcggttcgt caccttccag aacaggtcga tcggcgccag cgtgcgcagc gcgtcgctgc
      421 catctggcag ctccagcttg atgacatccg cgcccatgtc cgcacacagc gtggtgccaa
      481 agggcgcggc gagcacggtt gccatgtcga gaatgcggat gcctgcgagc ggtccggcgg
      541 aggaagtgcc agtttctgcg tggctgcgcg aggagagaga catgccgatc catgcaagtt
      601 gggaacgcag gcaagtctgc cgcccacccc gctcgcgccg caaccgcagt ttcgacatct
      661 tgaaccgtgt tcgccgctgc atggcggcgg gcgcgccggc tacaactgcc gccccagctg
      721 cgaagccacc tgttccacca ccttttcacg gaaggggcgg ttctcccaca tctccagcgt
      781 cacgcgcttt gacttggcca gatcagcttc gaatacctgc tgcatctgcg ccgcaagctg
      841 ccggtcgtag acattcaggc tggcctcgtc gttgatgcga aacgaacggt tgtcgaagtt
      901 ggtcgagccg accgaaacca tgaagccgtc tatcaccagc accttcacgt ggtacatggt
      961 cggctggtat tcggcgattt ccacgcccgc cgccagcaac tcgccccaac gcgcgcgcga
     1021 ggcgcgccgc accacttcca cgtccatgtg cggtccgggc acgatgatcg acacgcgcac
     1081 gccgcgctcg cgcgccttca ccagcgcctc cacagcgagc gcatcaggca cgaagtagga
     1141 acccgacagc aggatgcttt cctgcgccgc cgaaatcgcc agcagataca tcaggtgcat
     1201 gctttcgctg ccgcctgcag gagaactgct gaacatctgg gccggcgcct ggccgcgcgc
     1261 tgtcaggctc ggaaaatagt cctcggtata aagaacacgg ccggtggcgc gtgtccagtt
     1321 gtcgacgaag accgcctgca tctgcgcgac caccgggcct tccacccgga agtgggactc
     1381 gcgccaatgg tccgggccgt gcgcatcccc ggtccattcg tcgccgatgc cgacgccgcc
     1441 ggtgaagccg accttgccgt cgatgacgag gatgcgccga tgcgtgcggt tgttcatgcg
     1501 cggcaggttc cggatggtcg gcttgtgaaa gcgttcgacc tcgatgccgc cgtcgcgcat
     1561 ccggtcgatc agcgtcagat ccatcttcgc gctgccaacc caatcgagca gaacatgcac
     1621 cttgacgccc gagcgggcgc gctcgatcag tgcggcggca aagcggtcgg cgatctcccc
     1681 ggaccagtag atgtacatct cgaacgtgat cgtgcgctcg gcggaagcga tggcctcgag
     1741 catggccggg aagatgcgca cgccgttctc caggtgctgc acggaattgc cttcggcaac
     1801 cggcggcccg agcaggacgc ccatggtgcg caggaactgc ggctcggcca cgccgtagcg
     1861 atgctcgacc tgcgtgcgca aacgcgggtc ggcagtggtc aggttcgtga cgaccagcgc
     1921 gagaatgacc gtcacgatgg cggtccagac gatggtcctg atatgcgatg aacgcggcat
     1981 gggcgcaggt agcgtggttc gcctatgcta cgtaggcctc atgcttgcgt tcttgcgttt
     2041 gggtaagggt acgctcggta acggtggttg ccagcatcca gttgcgcgcc ggcatgcgct
     2101 ctacaagcct cccgggtcca tgtgacggat cgactcgcgt cgtttcgagg acgcctccgc
     2161 cttgccttga agattggcca gagcgcgcgc cagttgctcc gcagccccgg cgatggccag
     2221 ctccatgttc ggcgctcgat gctccacgga aatcggattc gcaccggcca ggcgcgcttc
     2281 gagctggcaa cgcttgtcgt cgtcgccggc cttggcgcta ttcacgtcat tcagatggac
     2341 ttcgatgcgt gtgatgcggt cgctgagatg cttgagttcc tgctccagca tctgttcaac
     2401 gcgctcttgc aggcttgggg acccgactac gtgtttgtcg gtgttgagct ggatttgcac
     2461 gactgcctcc tcgatcgatt gacaatgcca gtgtgatggc ggcaaatcgc ctcggttccc
     2521 gtgcgccggt cgccacgcgg cagagagcgc aacgggaagc cgccgccgcg agcggaattg
     2581 ctttccaatg ctgcgttgcc ctcgccatgt cgtccccgcg caggcgggga cccagctccg
     2641 acgtatgcag agaggttcaa acttgagctg gccccgcccg cgcgggagcg atatgaatgg
     2701 gaaatgtgaa cgacatcggc acacggcgcg cttgccgatg ccgacacaac gccactcccg
     2761 ccgcccggcc cgagccctca gtcgtcggcc gccccgtcga tgcccagttc ctggatcttg
     2821 cgcgtgatcg tgttgcggcc gatcccgagc cgcactgccg catcgttgcg gcggccgtga
     2881 gtgtggcgca acgccgtctt gatcagcgcc gattcgaact gccgcccgag cgcgtccatc
     2941 acttccggct ggccggcggc aagcatctgg gccgcctcgg cttcgagcaa ggaaatccac
     3001 ccggggcgca ctgccggcgc aagtcccggc tcgcgtgcgg cttcgatggt cgggacaggg
     3061 acaggactct cctcctgcgg cgccggcgcc tggctgccgt cggggcccgt cgcgctctgt
     3121 gtagagccgg ttgtttcgcc gcgcaccaga tcgggcggca gatccttgat ctccaccgtc
     3181 tgccccggcg ccatcaccgt gatccagttg cacaggttct cgagctggcg cacgttgcca
     3241 gggaagtcga gcccggtgag gtaatgcatc gccgcgtccg acatgcgctt ggcttcaacg
     3301 ccaagctgcc gtgcgctctg tgcgagaaag tagcgcgtga gtagcgggat gtcctcgcgc
     3361 cgttcgcgca ggctcggcag acgcaggcgg atcacgttca ggcgatggta caagtcctcg
     3421 cggaacaggc cgtcgcgcac gcgctgttcg aggttctgat gcgtcgccgc gatcacgcgc
     3481 acattcgcct tcaacggctg gtgaccgccg acgcggtaga agtggccgtc ggagagcacg
     3541 cgcagcaggc gcgtctgcag atcgaacggc atgtcgccga tttcatcgag gaagagcgtg
     3601 ccgttctccg cctgctcgaa gcgaccgcgc cgcatcgcct gcgcgccggt gaacgcgccg
     3661 cgctcgtgcc cgaacagttc ggactcgagc aggtccttcg gaatcgccgc ggtattgagc
     3721 gcgatgtacg gctgtgccgc gcgcgggctg tgcttgtgca gcgcgcgcgc gaccagctcc
     3781 ttgccggtgc ccgattcgcc ggtgatcagc accgtcacgt tcgattgcga cagccggccg
     3841 atcgcgcgga atacatcctg catggccggc gcctggccga ggatttcggg cgtgacgccg
     3901 ttggcttgtt cgacgtcggc ttcgcgcagg ctctcttcga gcgcgcggcg tatcagctcg
     3961 accgccttgt cgacatcgaa cggcttggcc aggtattcga aggcgccgcc ctggaatgcc
     4021 gcaacggccg agtcgaggtc ggagaatgcg gtgatgacga tgaccggcag ccccggatgc
     4081 atttccttgg cggcctgcag cagtgcgagc cccgattcgc ctggcatgcg gatgtccgac
     4141 accagcacct gcggcgcacc atcctggagc gccgccatcg catcgcgcac attcgagaag
     4201 ctcctggttg cgaggttttc gcgggcgagc gccttctcga gcacccagcg tatcgattcg
     4261 tcgtcgtcga caatccagat cggcttcatc ttgtgtcttg cgtccctgct atctgcatgt
     4321 ggttagggca gcgggatcag aacgcggaag tcagtgcatc ccggcctgct ctcgcattcg
     4381 atcacgccca tgtgctgctg cacgaaggtc tgagccaggg tcaaccccaa tccgcttccc
     4441 cccgcgcgtc ccgacaccag tggataaaac acgcggtcct gaatgtcagg cgggatgcct
     4501 ggcccgttgt caacgatatg caagtccaat gccagcctgt aacgaatttt cgcgagcgtg
     4561 atctggcgcg ccacgcgggt gcgcagcgtg atctgcgcgt cgccgtcggc gatcttttct
     4621 tcgagcgcct gggccgcgtt gcgcacgatg ttgagcaccg cctggatcaa ctgctccttg
     4681 tcgccgcgga attcggggac ggacaggtcg tagtcgcgcg tgatcgtcag gccgctcggg
     4741 aattcggcca gcatcaggct gcgcacccgc tcgcatactt catggatgtt tacgtcgccg
     4801 acgatgtgcg gatgcctgtg cggcgccagc agacgatcga ccagcgtctg cagccggtcg
     4861 gcttccttga cgatgacctg cgtgtactcg cgcagctcgc gcaggtgacg ttcgggcagc
     4921 tcgagttcca gcagctgcgc ggcgccgcgt atgccgccca gcggattctt gatttcgtgg
     4981 gcgagattgc ggatcagctc cttgttgagc tggctctgat cgagcaggcg ttcttcacgg
     5041 tccagcttga gctgctgtac gttctcgcgc agctccagca gcaccggcgt ggccgggatg
     5101 tcgagcgcgg tgacgatcag gtgcacgtgc agcggctcgc gcccggcgcg ctcaagggcg
     5161 agatcgagcc gcttgtcagc gaattcgtgc gcgcgggcct gttccagcac cgggtggagc
     5221 tggccgccgt tgaagaagag atcggcgatg cgctgctgcg cgagcatgcg gaacgaactc
     5281 tccagcaggt tctcggccgc ggcgttggca tagacaacgc ggtcttccgc gtccagcagc
     5341 aagaccgagg aagacaggag ttcgaggcct tcaagagagg gggggctgtg tttcacaaga
     5401 ggttcccgtc aatgatgcag ctagcttatc gcatcgcgtg ccgcagatgg tttgtccggc
     5461 gccggcacct gcggttcagg tcacgatgct tgccgtcggc gtgcgaagga cagccgtgga
     5521 cttttcgcat catcaacgac aaggggccgc aatgcggccc cttgaatgca agcacgatgc
     5581 ccactcagcg caggttgttg agttcccgct gcagcgcttc gatattcttc tcggtacgac
     5641 ggatgtcttc ctgcatcagg gccacgcgct cctggtactt cgcatagttg cgctcgtcgc
     5701 cgcgccgctc gggctcgccg ttgttgaact ccttcttcag gccggcgagc ttctgctcct
     5761 cggcgcgcat ctcgtcctgc aggatgcggc gccggtcggt gtcgcgcgcc cgctgggtcg
     5821 cgctgtcgac cttcgggaaa tccaccggcg cggcggccgg acgcgcagcg gcggtcggtg
     5881 cggcaggcgc cgcgcgcgca ggagcgggcg ccgaagccgg cgccggcacc atcgacaagc
     5941 caggcagatc gacccgcttg cagcctttcg tcatgccggt gttgcgatac tcgcggcggc
     6001 cgtcctcatc gacgcagaca tagacctctc cctgggcatg cgcgacgccc gcgaaaccgg
     6061 agaggagaag cagcagggat agttgtttct tcattatgtt gcttctaaga aactgcagcg
     6121 cttactagtt taggataaga atcaaagcat tacaaacgga tgctgcccat ccgtcataag
     6181 attgccacgg cgcaaaacag ttcccgacac acaagatcgc cagcctgtcc cgaaaaaaaa
     6241 aagcgggaaa ggcttgcgcc cttcccgctt cgttccgcgg agactcgatt acagcgagta
     6301 gtacatgtcg aactcgaccg ggtgggtcgt catgcggaag cgctggactt cctgcatctt
     6361 cagttcgatg taggcgtcga tcatcgtgtc gctgaacacg ccgccgcggg tcaggaactc
     6421 gcggtccttg tcgaggtact cgagtgcctg gtcgagcgac gagcacacag tcgggatcag
     6481 cgcatcttct tccggcggca ggtggtacag gtccttcgaa gcggcttcgc ccgggtggat
     6541 cttgttctgc acgccatcga gacccgccat cagcagcgcc gagaagcaca ggtaggggtt
     6601 cgacagcgga tccgggaagc gcgcctcgac gcggcgcgcc ttcggattgg ccacgtgcgg
     6661 aatgcggatc gatgccgagc ggttgcgggc cgagtaggcc agcttgaccg gcgcttcgaa
     6721 gcccggcacc aggcgcttgt acgagttggt gcccgggttg gtgatcgcgt tcagcgcgcg
     6781 ggcgtgcttg atgatgccgc cgatgtagta cagcgcgaag tccgacaggc cggcatagcc
     6841 gtcgcccgcg aacaggttct tgccgtcctt ccagaccgac tggtgcacgt gcatgccgga
     6901 gccgttgtcg cctacgatgg gcttgggcat gaaggtcgcg gtcttgccgt aggtgtgggc
     6961 gacgttccag atcacgtact tcagggtctg cgtccagtcg gcgcgctcga ccagggtcga
     7021 gaacttggtg ccgatttcgt tctggccggc gcccgccact tcatggtggt gcacttcgac
     7081 cgggatgccg agcgcttcga ggatcaggca catttccgag cgcatgtcct ggaagctgtc
     7141 gaccggcgga accgggaagt agccgccctt gacggtcgga cggtggccgg tgttgccgcc
     7201 ttcgaactcg cggtcggtcg accaggacgc ctcttcggag tcgaccttca cgaagcagcc
     7261 cgacatgtcg atcttccagc gcacgccgtc gaagatgaag aattcgggtt ccggaccgaa
     7321 gtaggcggtg tcgccgatgc cggaagactt caggtaggct tccgcgcgct tggcgatcga
     7381 gcgcggatcg cggtcatagc ccttgccgtc ggaaggctcg atcacgtcgc actgcatgaa
     7441 cagcgtggtc tcttccatga acgggtcgat gttggcggtg ttcggatccg gcatcagcag
     7501 catgtcggac gcttcgatgc ctttccagcc ggcgatcgag gaaccgtcaa acgcatgacc
     7561 ggactcgaac ttgtccatgc cgaactgcga gaccggcacg gtgacgtgct gttccttgcc
     7621 gcgggtgtct gcgaaacgga agtcaatgaa cttgacctcg ttttccttgg ccattttcaa
     7681 gacctcttcg gccgtccttg ccatgcgaat ctcctaattg agcaaaagtg tggaatttta
     7741 gaaccgtccc tggcgggcgt cgaacgccgg cgcaaagcgg gtggatcgtg gccggattta
     7801 gcagtttccg tgccaccaca tttttcacga cggtgccgtc gagttcgcga ggatcggcgc
     7861 ggtttgaggc ctgtttgaac gctatcgcgg catccgcgta caagaccagc ccggccacgt
     7921 ttcaccgaaa ccgtgcgtgt tgactgtacg cgcactattt tagttcacct acgcaccatt
     7981 tttgtgcgcc gacatccggg gcagcgcgcg ctagaatgaa ggcctcgatc accaaggagc
     8041 aagcatgagc ctcaatattc tcgagaccgc acgtgaacgg ggccgccagc acaacctgcc
     8101 ctacgcgggt gcgctcacgc cccaggaagc ctacgcgctg ctgcagcagg aaaagtcggc
     8161 gatgctcgtc gacgtgcgca cgaacgccga acgcgactgg gtcggccgcg taaagatcga
     8221 cccggcccaa catgcggcga tcgaatggag cctgtacccg ggcggcacgc cgaaccctgc
     8281 tttcctcgac cagctccaat ccgccgcaca gaaggatgac gttctgctgt tcctgtgccg
     8341 ctccggcgtg cgctcgcgcc atgcggccag gctcgcaacc gagcacggct tcttgaactg
     8401 cttcgacatt ctggaaggct tcgaaggcga caaggatgcc aacggccatc gcaagaccgt
     8461 ctgcggctgg tgcaaggccg ggttgccctg ggtgggcgcg taggctccaa caccgctagg
     8521 ccggctcccc ggctcgctct tccatcagcg ccgcgatccg ccggcgcacg aagccgatgt
     8581 ggctgcgcag cccgtacagc tcgtcggcga acgagagcgg gatcgcgatg tggttcaccc
     8641 ggtcctcgat ctcgttcagg cgttcgagct gttcgcgata cacgtcggcg cggcgatcct
     8701 gcggcacctc ctccagcgcc tgctccaccg cgcgcaactg gccgtaccag cgatagacgc
     8761 gcgaacgcac ctgccacaca tacagcggcg ggacgatgcg cgatagcggc agcatcagcg
     8821 cgcccagcgc caccacgatc acccacatcc tgtcgaagag gttggccagc cagaacggca
     8881 ggtagcgctg caggaacggc ggcccgctgc ggtagtactt ctgcgccgac gacgcgaccg
     8941 ggatctcggt gtagttcgca ttcgggaatt cgccgcgccg ctggaaccag ccggcatcgt
     9001 tgtggatctg cgcggccgcg tgcacgaaca gatcgacgag ggccggatgc aggtcggcgc
     9061 gcgcgaccag tgtcgctgtc ggcgcgatca ggtgatagtc ccgcgcgggc aggtcgcggc
     9121 ccaggtcgac gatcccgcgc ggcagcacca catgggaaag gaaaggcaga cggcgcgtgt
     9181 aggcctcggc ctgggcaaaa tcgaacaggc ggattcccgg cgtctgcagc agcatctgga
     9241 ccagcggcgc gtcgggcgcc gagctgaaca cgagcgcgtc gatttgacct tcgagcagcg
     9301 cgaccgtcgc aggggtgttc tccagcccgc ttagcgccag ctcacccggc tcgatgccgt
     9361 tgaccgccag gatgcgcttg aacagctgcg gcacgccggt gccttccggc cccacgttga
     9421 ccttcatacc gcgcagctgg gacagtcggg tgaccgcctt cctttcgcgc atgaacagcc
     9481 agaccggctc ggtaaacagg cttcccaggg acaccagccc ctgacgttcg gcctgggcca
     9541 tttccgtcga accgctctgc acgaatgcaa tgccgacgcc ggagcggggg tcgacaagac
     9601 gatcgagatt ctcttgcgag ccgagcgagc gaacgaggtc gaccttcacc cccatgcgcg
     9661 cgagctgggc ggcgtaacgc ttgccgattt cttcataggc gctgttctcc tgtccggtcg
     9721 acagcgtcac ctttggaggc ggcgaaggat cgacgatcca gtaggcaagc agacaggccg
     9781 ccgccaggag catgagcacc ggccccgccg agaacagcag gccgcgcagt gagatacggg
     9841 agaacgaaaa gatccttgac atggaagagc ctggaaaatc caggccacac catgccaaga
     9901 aacctgcgtg gaggcaagca ggagtgtgct gcgcgcgttg gcggggctgt aaccgagggt
     9961 atcagtgcat gtcgcggcgg gcacccggat gggcgtaccc gcggttgggc cgccctgaac
    10021 gccggccggg agcgctgtca cgtgaaagga agtcggaagc aagccggaac cagtcctcca
    10081 ccgacagcag ccggcgcgcc agcggcagca gtgactgctc ctccagcgtc aggcgcgaag
    10141 cggcctggct gcaataggca gacatcgatt cgcacacgcg actgacctgc agacggccga
    10201 aatcggtgcg cgaggccagc agcgcgcccg cttcacgcag atacccgatg gatttgtgcc
    10261 gcaggctctc catttcggcg atcagcaact cggcctcgcg tcccgtgcct ctcagcaccg
    10321 gaatcagaaa acgttcgatc ttccgctcgc gaaggtattg atcgaactgc agcaggcgat
    10381 ggtagcccga ctcgagaaaa ccaaaatcga cattttgcag gccgctccat gcggcatcga
    10441 gaaactgctg cagccggttg agcatcgtgc gtgtcttttc ctgctcggct gcaatggcga
    10501 cgagagaata ggtggcggtc agcattgagg gctcctggat tcttcggtgc gctggttgaa
    10561 agttggggaa gtataggcgt ggggatatgt ccgccgttga tcttcctcaa atgcgttcga
    10621 gccctgcttg aagccaggaa ttgcggcatg ccgcaaggca tgccgccgca tcactccagc
    10681 cgggccgcgt gttcgcgtgt ggagtggaag gtcagctgcg gccagcgctc ctgcgtcagg
    10741 cgcagattca ccccggaggt ggcgaggtag gcgagattgc cggccgcgtc gtgcgcgagg
    10801 ttggcaccgg ccgacgagcg ttcgaagtcg gccagcgcct tcttgtcatc ggaggagatc
    10861 cagcgcgcgc tgctgatgct ggtgccttcg aacaccgcgt ccacgccgta ctcgttcatc
    10921 aggcggctcg cgacgacttc gaactgcagc acacccaccg cgcccaggat caggtcgccg
    10981 ccatgcaccg gcttgaacac ctgcaccgcg ccctcctcgc ccaactgctg caggcccttg
    11041 tgcagctgct tgatcttcaa cggattgcga atgcgcacca gccggaagaa gtcgggggcg
    11101 aaatacggaa tgccggtgaa ctgcagcatc tcgccttcgg aaaagctgtc gccgatctgc
    11161 atgttgccgt ggttcggcag gccgatgatg tcgcccgcat aggcttcctc gacctgctcg
    11221 cggctggaag ccatgaaggt caccacgctt gacaccttga tctcgcggtt caggcgcaga
    11281 tgcctgacct tcatcccgcg ctcgaatcgg ccggagcaca cgcgcaggaa ggcgatgcgg
    11341 tcgcggtggg ccggatccat gttggcctgg atcttgaaga cgaagccgga gaacggctcc
    11401 tcgaccggct cgacctggcg tacggtggcg tcgcgcgagc gtggcgccgg tgcccagtcg
    11461 agcagcgcgt tgaggatttc gcgcacgccg aagttgttga tcgccgaacc gaagaacacc
    11521 ggcgtctgcg tgccggcgag gaaagcatcc aggtcgaacg ggtgcgaggc accgtgcacc
    11581 agttccacct ccatcttcag ctgctccatc tccatcggga acagctcggc caggcgcgga
    11641 ttgtcgatgc ccttgaccac ctcgaactcc tggtccgcgc gctcctcgcc ggccttgaac
    11701 agcaggattt cgtcgcgcag caggtgatag acgccgcgaa aattcttgcc catgccgatc
    11761 ggccaggtca ccggcgcgca ctggatcttc agcaccgatt cgacttcgtc gagcagttcg
    11821 agcggatcgc gcgtctcgcg gtccagcttg ttcatgaagg tcaggatcgg ggtggcgcgc
    11881 atgcggcaga cattgagcag cttgatcgtc tgctcttcca cgcccttggc cgcgtcgatc
    11941 accatcagcg ccgaatcgac cgccgtgagc acacgatagg tgtcttccga aaagtcttcg
    12001 tggcccggcg tgtcgagcag gttgaccacg tgctcgcggt attcgaactg catgaccgag
    12061 ctcgcgaccg agatgccgcg ctgcttttcg atctccatcc agtccgaggt tgcgtggcgg
    12121 ccgctcttgc gcgccttgac ggtcccggca agctggatcg cgccggagaa cagcagcagc
    12181 ttttcggtca gcgtggtctt gccggcgtcc gggtgggaga tgatgccgaa ggtgcggcgg
    12241 cgcgcgactt cgcgagcgat cagttcggcg ggtttgcgcg gcgcggatgc ggcgtcaacg
    12301 gtgtcagggg tttcggtgat ggaagccatg ttgcggcgag aatgaaaacg tccctgccgg
    12361 gcagggactt ggacggctga aaacgatgca gacagtgcaa cggtcgcgtt gctcagcgct
    12421 ggatgaaatg ttcgcggtag taacgcagtt cctcgatcga ctcgagtatg tcggccagcg
    12481 ccgtgtgctt ctggtgcttc ttgaagccgg aagccacttc cggcttccag cgcttgcaca
    12541 gctccttcag cgtggagacg tcgagattgc ggtagtggaa gaacgcttcc agcttcggca
    12601 tgccgcgcgc catgaagcgg cggtcctggc agatcgtgtt gccgcacatc ggcgatttgc
    12661 cggccggcac gaaatgcttc aggaaatcga tcagcgcggc ttcggcatcg gcttcgctga
    12721 ccttggacgc cttgacccgg tcgatcagac cggagcgccc gtgggtgccc ttgttccacg
    12781 catccatgcc gtcgagcact tcgtcgggct gatgaatggc aaaaacggga ccttccgcca
    12841 acacattgag ttccgaatcg gtaaccacaa cggcaacttc gatgatgcgg tcggtgtcgg
    12901 gatcgaggcc ggtcatttcc atgtcgaccc agaccaggtt gaactcgttc ggccgcgcgg
    12961 gagccgcggc aggccgctcg gaaggggtgg cttgtgacat aatttatcct cttgaatcca
    13021 gcattttctc acaggcaggg catgacctca cttgcgttct ccatcttgtt cgtcggcttc
    13081 ctgctcctga ctctttcctt gcgtttctgg ctggcttccc ggcacatccg ccatgtccag
    13141 cgccaccgcg acgccgtccc cgccgagttt tcccacaaga ttccgctgta cgcgcaccag
    13201 aaggcggccg actacacagt cgcgcgcaca cgcttcgggc tcgtcacgat gatcgtcaat
    13261 gccgcggtcc tgatcggctt cacgctgttc ggcggcctgc aatggctgtc ggttgccgtg
    13321 ttcgacgcga ccgggccggg aatgtggcac cagctcgggc tggtggcggc atttgccgtg
    13381 atttccggac tggtggacct gccgttcgac tactaccggc agttcggtct cgaagaacgc
    13441 ttcgggttca acaagatgac gccgaagctc ttcttcagcg acctggtcaa gagcgtgctg
    13501 cttggcgtgg cgatcggcct gccgctgctg tgggtgattc tgatgctgat ggagcaggcc
    13561 ggcgatctct ggtggttcta cgcctggctg gtctggatcg cgttccagct gctgatgctg
    13621 gtgctgtatc cgaccgtgat cgcgccgatg ttcaacaagt tcacgccgct cgaggatgcc
    13681 agcctgcgcg agcgcatcga aggactgatg aaacgcgtcg gcttcgcgtc gaagggcctg
    13741 ttcgtgatgg atggctccaa gcgcagcgcg catggcaacg cctatttctc cggcttcggc
    13801 gccgccaagc gcatcgtgtt cttcgacacc ctgctcgcgc gcctggcacc gcaggagatc
    13861 gaagcggtgc tcgcgcacga gctcggacat ttcaagctgc gccacatcat caagcgcatc
    13921 gccgtcatgt tcggcctgtc gctcgccttc ctggccctgc tgggttatct gaagaaccag
    13981 gtctggttct acaccggcct cggcgtggat cccatggttc ctggcaacaa tgacgcgatg
    14041 gcgctgatcc tgttcatgct ggtcctgccg gtgttcacct tcgtgttcgc gccgctggcc
    14101 tcgctgacct cacgcaagca cgagttcgag gccgacgcgt tcgcggcccg gcatacggat
    14161 ccgcaggacc tcgtatccgc gctggtcaag ctgtacgagg acaatgcgtc gaccctcacc
    14221 ccggatccga tccactcggc gttctacgac tcgcacccgc cggccagcgt gcgcatccag
    14281 cacctgctcg cgcagcagca ctgatggagc cgatgcgcgg taccgtcatc gccgcccacg
    14341 gacgccacta tctggtccag gccggcgaac agcgcctgaa ctgcgtgacc cgtggcaaga
    14401 agagcgatgt cgcgaccggt gacatggtgg agatcaagca gacctcgcag gatcagggcg
    14461 taatcgagaa gatcggcgag cgcaggaccc tgctctatcg ctcggaccag tacaagtcca
    14521 agctgctggc ggccaacgtc acgcagctgt tcatcgtggt cgccaccgag cccggttatt
    14581 cggacgacct cgtctcgcgg gcgctggtcg ccgccgaggc ggctggcgtg gcgccacacc
    14641 tgatcctcaa caagatcgac gttaccgcgt cactcgataa gacgcgcgaa cgcctgcgca
    14701 tgtatgcggc catcggctat ccgatccatg aagtgtcggt acgcacgcaa cccgagcaga
    14761 cgcgcgcact gctgatgccg atgttgcaag ggcatgcgac gatcctgatc ggccagtccg
    14821 gcatgggcaa gtcctcgctg atcaacctgc tggtgccgga tgcggacctg gccacacgcg
    14881 agatttccga cgcgctcgac tccggcaagc acaccaccac cttcacccgc ctgttcgcgc
    14941 tcgatgcgca cacggccgtg atcgactccc cgggcttcca ggaattcggc ctctatcacc
    15001 tgaccgaggg catgctggaa cgggcatttc cggagttcgc cccgcatctg ggccaatgcc
    15061 gtttctacaa ctgccatcac ctgagcgaac cgggctgcgc ggtgctggga gcggttgaaa
    15121 ccggtgccat ccatccgatg cgccacgcgc tgtaccagca actggtgcac gagtcctcgc
    15181 agaaaatcgg ttactgaggc gcgccaccgt ttgcatgcgg ctatcttttt ccgagaaaat
    15241 ccccttataa ttgaacaagt tacaaactcc ggcggcaggc gacaaccaag ccgccgtttt
    15301 catttatggc gatcaaacac ttcctgcagg tttccgactt cacgctggat gaattcgaac
    15361 acgtgatcga gcgcgccagc gtgatcaagc ggaagttcaa gaactacgag ccctaccacc
    15421 cgctggtcga ccgcacgctc gtgatggtgt tcgagaaaaa ttcgacgcgc acccgcctgt
    15481 cgttcgaagc cggcatgcat cagcttggcg gcgccgcaat ctacctgaac acccgcgaca
    15541 gccagctcgg ccgtggcgag ccggtcgaag atgccgcgca ggtgatgtcc cgcatgtgcg
    15601 acatcatcat gatccgcacc ttcggccaag acatcatcga gcgcttcgcc gcccattcgc
    15661 gcgtgccggt gatcaacggc ctgaccaacg agcatcaccc ctgccaggtg ctggccgaca
    15721 tcttcaccta catcgaacac cgcggctcca tcgccggcaa gacggtggcg tggatcgggg
    15781 acgccaacaa catgctgtat tcatggctgc aggccgcgca ggtgttcggc ttccacgtca
    15841 acgtctccac gcccaagcgc tacgagatcg atccggccct ggtggcgccc gacaacagca
    15901 gctataccgt gttcgccgat ccttccgacg cctgcgagaa cgccgacctg gtcactaccg
    15961 acgtctggac cagcatgggt tacgaggaag agaacgccgc gcgcctgaag gcgttcaagg
    16021 gctggat
//
LOCUS       DATLGF010000112         3594 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_161580, whole genome shotgun sequence.
ACCESSION   DATLGF010000112 DATLGF010000000
VERSION     DATLGF010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161580"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..889)
                     /locus_tag="VM406_03350"
     CDS             complement(110..889)
                     /locus_tag="VM406_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007583737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL75027.1"
                     /translation="MQGHHMNATSFNGKSVLVTGAASGIGRAAALAFAQQGAQVAVCD
                     IDAAGGEAVAAEIAQEGGTAIFIKADVSRSADVETLMARVVDTYGRLDCAFNNAGIEI
                     EHLPLAEADEATFDRLMSVNVKGVWLCMKHEIRQMLKQGGGAIVNTASVAGLVGAPLQ
                     PVYAATKHAVVGLTKTAAAEYGRFGIRVNTVCPGIIRTPMLERALEREPRREKTITKV
                     HPIGRLGEAQEIANAALWLCSDSASFVTGHQLAVDGGLTAV"
     gene            complement(909..2126)
                     /locus_tag="VM406_03355"
     CDS             complement(909..2126)
                     /locus_tag="VM406_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVL75028.1"
                     /translation="MDFDYSPKVKDLQKRLMAFMDEHIYPNEAKFFAEIEQNRANGNA
                     WVPTKLMEELKEKARAAGLWNLFLPASTRGAGLTNLEYAPLCEIMGRATWSAEVFNCS
                     APDTGNMETLERYASEELKQQWLEPLLEGKIRSCFAMTEPAVASSDATNIEASIVRDG
                     DEYVINGRKWWSSGANDPRCKVFIFMGKTDPSAEKHRQQSMIVVPRDTPGITVLRHLP
                     VFGYDDAPHGHGEVLFENVRVPASNILLGEGRGFEIAQGRLGPGRIHHCMRLIGLAER
                     ALEDMCKRTLKRVAFGRPVAEQTVTLERIANARILIDQARFLVLNAAHMMDTVGNKVA
                     AKEIAMIKVAAPNMACQVIDWAIQAHGGGGVSDDFVLAYSYAQARTLRLADGPDEVHR
                     NQIGKMELKKYRQ"
     gene            complement(2202..2900)
                     /locus_tag="VM406_03360"
     CDS             complement(2202..2900)
                     /locus_tag="VM406_03360"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HVL75029.1"
                     /translation="MGQIYLIRHGQASFGSADYDALSALGEEQARLLGRWFAHAGQRF
                     SCVLSGDLKRQRQTAALCMETLYGSDVPVCEVDGGFNEYDHHEVLVRQRPEFADPNEV
                     RRFLAASANPRAAFQDVFAAAMSRWMSGRHNAEYGETWPQFSARCVAAVERLFERGRG
                     QTIAVFTSGGTISAICQRVLGMSDAHTARLSWGLVNSGVTRLLYQPGQLSLSYLNSYA
                     HLEWQGRADALTWR"
     gene            3004..>3594
                     /locus_tag="VM406_03365"
     CDS             3004..>3594
                     /locus_tag="VM406_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL75030.1"
                     /translation="MHISRIDLNLFTVFEAIFSEGSVTRASERLNLTQPAISHALGRL
                     RQLFDDPLFVRQGHAMVPTPLARQVIEPVRQALRGLEVTLAGVQAFEPASASKRFNLA
                     VRDVLETTVLPPLMRRVRADAPGVDIAAMHVERRELEHELASGTIDGAIDVLLPLSPD
                     LRHTRIFRDSTVVVARRGHPEIDGTLDLAAYLRQDHV"
BASE COUNT          574 a         1217 c         1102 g          701 t
ORIGIN      
        1 cgattttccg gcctcgattt tccggcctcg attttccggc ctcgattttc cggcctcgat
       61 ttttcggcct cgcttctcga acgcctactc gttccgcctc gctcccggtt cagaccgccg
      121 tcaagccgcc atcgacggca agctgatggc cggtcacgaa cgatgcactg tccgagcata
      181 gccaaagtgc ggcgttcgca atctcctgcg cctctcccag gcggccgatg ggatgcactt
      241 tcgtgatcgt cttttctcgc cgcggttcgc gctccagcgc gcgctcgagc atcggggtgc
      301 ggatgatgcc tggacacacg gtgttgacac ggatgccgaa gcgcccgtat tccgcggcgg
      361 cggtcttggt gaggcccacc accgcgtgct tggtggccgc atacaccggc tgcaacggcg
      421 ccccgaccag gcccgcgacg gaagcggtgt tgacgatcgc gccgcctccc tgcttgagca
      481 tctggcggat ctcgtgcttc atgcacagcc acacgccctt gacgttcaca ctcatcaagc
      541 gatcgaaggt cgcctcgtct gcctcggcca gcgggaggtg ctcgatctcg atgccggcat
      601 tgttgaacgc gcagtcgagg cggccgtagg tgtcgacgac ccgcgccatc aaggtttcca
      661 cgtccgccga tcgtgatacg tcggccttga tgaagatcgc cgtaccgcct tcctgcgcga
      721 tctccgccgc taccgcctcc ccgccggcgg cgtcgatgtc gcagaccgcc acctgcgcgc
      781 cctgctgtgc gaatgcgagc gcggccgctc gcccgatacc gctggccgcg ccggtgacca
      841 gcaccgactt gccgttgaac gatgtcgcat tcatgtgatg cccctgcatt gtcgtgttcg
      901 aatggtgctc attgcctgta cttcttcaac tccatcttgc cgatctgatt gcggtgcact
      961 tcatccgggc cgtctgccag ccgcagcgtg cgcgcctgcg cataggagta ggcaaggacg
     1021 aagtcgtccg acacgccgcc gccgccgtgc gcctggatcg cccagtcgat cacctggcag
     1081 gccatgttcg gcgcggccac cttgatcatc gcgatctcct tcgcggcgac cttgttgccg
     1141 acggtgtcca tcatgtgcgc ggcattgagc acgaggaagc gtgcctggtc gatcaggatg
     1201 cgcgcgttcg cgatgcgctc tagcgtcacc gtctgttcgg ccaccggcct gccgaacgcc
     1261 acccgcttca aggtgcgctt acacatgtct tccaatgcgc gctcggccag gccgatcagg
     1321 cgcatgcagt ggtggatgcg gccgggcccg agccgccctt gcgcgatttc gaagccgcgt
     1381 ccctcgccaa gcaggatgtt ggaagccggc accctcacgt tttcgaacaa gacctcgccg
     1441 tggccgtgcg gggcgtcgtc gtagccgaat accggcaggt ggcgcagcac cgtgattccg
     1501 ggcgtatcgc gcggtaccac gatcatcgat tgctgcctgt gcttctcggc gctcgggtcg
     1561 gtcttgccca tgaagatgaa caccttgcag cgcggatcgt tggcgccgga cgaccaccac
     1621 ttgcgtccgt tgatcacgta ttcatcgccg tcgcgcacga tcgaagcttc gatgttggtc
     1681 gcgtccgacg aggcaaccgc cggctcggtc atcgcgaagc acgagcggat cttcccctcc
     1741 agcagcggct ccagccactg ctgcttgagt tcctcgctcg catagcgttc cagcgtttcc
     1801 atgttgccgg tgtccggcgc ggagcagttg aacacttcgg ccgaccaggt cgcgcgtccc
     1861 atgatttcgc acagcggggc gtattcgaga ttggtcaggc cggcgccgcg cgtggacgcg
     1921 ggcaggaaaa ggttccacag gcccgccgcg cgcgccttct ccttcagttc ctccatcagc
     1981 ttcgtcggca cccaggcatt gccattcgca cggttctgct cgatctcggc gaagaacttc
     2041 gcctcgttcg gatagatgtg ctcgtccatg aacgccatca ggcgcttctg caggtccttg
     2101 accttcgggc tgtagtcgaa gtccattcgt tactccttgg ttatgcgcgc gaggcgcggg
     2161 ctgtggtgct acttcggtgc gatgttgcgc gcgctggcgc gtcaccgcca tgtcaatgca
     2221 tcggcgcgcc cctgccactc gaggtgcgcg tagctgttca ggtatgacag gctgagctgt
     2281 ccgggttgat agagcagacg cgtgacgccg ctgttgacca gaccccagct caggcgcgcg
     2341 gtatgcgcgt cgctcatgcc gagcacgcgc tggcagattg cggaaatggt gccgccggag
     2401 gtaaacacgg cgatcgtctg gccgcgcccg cgttcgaaca ggcgctcgac ggccgcgaca
     2461 caacgcgcgg aaaactgcgg ccaggtttcg ccatactcgg cgttgtggcg gccgctcatc
     2521 cagcgcgaca ttgccgcggc gaacacatcc tggaatgccg ccctcggatt ggcgctcgcg
     2581 gcaaggaagc gccggacctc gttcggatcc gcgaattccg gccgctgccg cacgaggacc
     2641 tcgtgatgat cgtattcgtt gaacccgcca tcgacttcgc agaccggcac atcgctgcca
     2701 taaagcgtct ccatgcagag cgccgccgtc tggcgctggc gcttcaggtc gccggagagc
     2761 acgcacgaga aacgctgccc cgcatgggca aaccatctgc cgagcaggcg cgcctgctct
     2821 tctcccagcg cagacagcgc atcgtagtcg gcactgccga aggaagcctg gccgtgtcgg
     2881 atcaggtaaa tctgtcccat agcaactctc gctcgcgccg attggtcggc tgttacgaca
     2941 ggagcatagc cgccgatgta aaatcgatca aatgaatagt tattattctc cagcataagt
     3001 acgatgcata tttctcggat cgatctgaac ctgtttaccg tcttcgaggc gatcttcagc
     3061 gaaggcagcg tcacccgcgc gagcgaacgc ctgaacctga cccaaccggc gatcagccat
     3121 gcgctcgggc gcctgcggca gctgttcgac gatccgctgt tcgttcgaca ggggcacgca
     3181 atggtgccta ctccactggc gcgccaggtg atcgagccgg tacggcaggc gttacgcggg
     3241 ctggaagtca cgctcgccgg cgtgcaggcc ttcgaaccgg ccagcgcatc caagcgcttc
     3301 aacctcgccg tgcgtgacgt gctggaaacc acggtgctgc cgccgctgat gcggcgcgtg
     3361 cgcgccgacg cgccgggcgt ggacatcgct gccatgcatg tggaacggcg cgaactcgag
     3421 cacgagctcg ccagcggcac gatagacggc gcaatcgatg tcctgctgcc actgtctccg
     3481 gacctgcggc atacgcgcat cttccgcgat tcgaccgtcg tggtcgctcg ccgcggccat
     3541 cccgagatcg acggcacgct cgatctggcc gcctacctgc gccaggatca tgtg
//
LOCUS       DATLGF010000113         3354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_161649, whole genome shotgun sequence.
ACCESSION   DATLGF010000113 DATLGF010000000
VERSION     DATLGF010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161649"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..815)
                     /locus_tag="VM406_03370"
     CDS             complement(150..815)
                     /locus_tag="VM406_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL75031.1"
                     /translation="MLTLHDVSKSYAGRKILSGLSYTLHDGEYVAVMGESGVGKSTLL
                     NLIAGLDAPDAGDIVIDGVSLAGIDDDAATRLRRKQMGFVFQAFHILPHLTLAQNVAL
                     PLLLNRAPPQQRVLAMLEAVGLGPRADDFPRQLSGGELQRVAIARALVHRPRVLLADE
                     PTGNLDPDTAADVLELLRREIKQNGGCAIMVTHSPVAAASADRVLLLTRTGLQPHVPK
                     GHA"
     gene            991..1968
                     /gene="egtD"
                     /locus_tag="VM406_03375"
     CDS             991..1968
                     /gene="egtD"
                     /locus_tag="VM406_03375"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HVL75032.1"
                     /translation="MQALSQSDLCSHGFIQLWSPDTRAIRAEIIAGLQAGQAAIAPKY
                     FYDALGSRLFEAVCELPEYYPTRTEAAVLESSLMQIARAVGRGGTLIDLGAGNCAKAA
                     RLLPALQPSQYVPVDISVDFLAEAVERLRHRFPRLPILGIGMDFAEELALPEAVATHQ
                     RLFFYPGSSIGNFTPSQALHLLRRIRAAGGDLLIGVDLVKDMAPLEAAYDDALGVTAA
                     FNRNLLLHVNRLIGADFAVEDWQHRAFFNAAESRIEMHLVARRDLAVRWQGGERAFVH
                     GESIHTESSYKYTRERFFSLLEAAGFRHLRSWEDPRGWFMVCLASEERR"
     gene            1965..3197
                     /gene="egtB"
                     /locus_tag="VM406_03380"
     CDS             1965..3197
                     /gene="egtB"
                     /locus_tag="VM406_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HVL75033.1"
                     /translation="MTAALYDRFRRVRAETLALAAPLSEEDCCVQSMTDASPVKWHLA
                     HTSWFFETFILERFERPFHAFQPAFRMLFNSYYNGVGDKHPRPQRGMITRPGLAEVLA
                     YRRNVDERMAMVIGEGAASSGLGELVELGLQHEQQHQELVLTDLKHLLSMNPLKPAYA
                     QRGAVHATLAPEHRWIGFDGGVVHIGHAGDGFCFDNELPRHRQFVEPYALSSRLVTNA
                     EYLAFIEDGGYRNPAVWLAEGWDWVRNGGNGESGGSGAIEAPLYWHRAESGWQEFTLH
                     GMQALEPDRPATHLSFYEADAFARWAGARLPTEAEWEHAAASCPGDIDPCGIAQLFGV
                     SWQWTSSSYSPYPGFTAPRGAIGEYNGKFMCNQYVLRGSSIATPAGHTRISYRNFFPA
                     YARWQFSGIRLARSAWKA"
BASE COUNT          570 a         1071 c         1120 g          593 t
ORIGIN      
        1 attaaatgcc gcggcgttga tcagatagat cgcgaaaccc agcgcgacac cgatcgcaat
       61 cgcggtcagt gcgaccaggt tgcgcagcgg atgggcacgc cactccccct gcaggagcca
      121 gcgcgccagc acgccgggaa ccgcctcgct catgcatggc ccttcgggac atgcggctgc
      181 aacccggtgc gggtgagaag cagcacgcgg tcggcactgg cggcggcgac gggggagtgc
      241 gtgaccatga tcgcgcagcc tccgttctgc ttgatctcgc gccgcagcag ttcgagcaca
      301 tccgcggccg tatcgggatc gaggttgccc gtcggttcat cggcaagcag gacgcgcgga
      361 cggtgcacca gcgcccgtgc gatcgccaca cgctgcagct cgcccccgga cagctggcgc
      421 ggaaagtcgt cggcgcgcgg gccaagcccg accgcctcca gcatcgccag cacgcgctgc
      481 tgcggggggg cgcggttcag cagcagtggc agggccacgt tctgggccag cgtgagatgg
      541 ggcaggatgt ggaatgcctg gaacacgaag cccatctgct tgcggcgcag gcgggtcgcg
      601 gcatcatcgt cgatcccggc cagcgagacg ccgtcgatca cgatgtcgcc ggcatccggc
      661 gcgtcgagtc ccgcgatcag gttgagcagc gtcgatttac cgacgccgga ttcgcccatc
      721 accgcgacat attcgccgtc gtgcagcgtg tagctgagtc ccgaaagaat tttccttccg
      781 gcataggact tggaaacgtc gtgcagggtg agcatggatc gaacgcaaca atgggaacgg
      841 ggatggcggc acgcaactca cagcagcagg cagttcctcg cccgccgttg tgtcggctca
      901 agtgaggtgc ggcagtgcct gcctatgata gccacaaccc cttgccgccg ttcatcctcg
      961 cgcggcccct caacctttct gactttcctc atgcaagcgc tgtctcaatc cgatctctgc
     1021 agtcatggct tcatccagct ctggtcgccg gacacaaggg cgattcgcgc cgaaatcatt
     1081 gccggactgc aggccgggca ggccgcgatt gcaccgaagt acttctatga cgcgctcggc
     1141 tcccgcttgt tcgaggccgt ctgcgaattg cccgagtatt acccgacgcg caccgaggcc
     1201 gcggtgcttg aatcctcgct gatgcagatc gcacgcgcgg tgggacgcgg cgggacgctg
     1261 attgacctgg gcgccggcaa ctgcgcaaag gcggcgcgac tgctcccggc cctgcaaccc
     1321 tcgcagtacg tgccggtgga catttcggtg gacttccttg ccgaagcggt cgagcgactg
     1381 cgccaccgtt tcccccgtct tccgatactc gggatcggca tggatttcgc ggaggaactg
     1441 gcgctgcccg aggcggtggc cacgcatcag cgattgtttt tttatccggg ttcgtcgatc
     1501 ggcaacttca cgccgtccca ggcgctgcat ctgctgcgcc ggatacgcgc cgccgggggc
     1561 gacctgctga tcggcgtgga tctggtcaag gacatggcgc cgctggaggc ggcctacgac
     1621 gatgcgctcg gggtgacggc ggcgttcaat cgcaacctgc tgctgcacgt gaaccggttg
     1681 atcggcgccg acttcgccgt cgaggactgg cagcatcgcg cgttcttcaa cgccgccgag
     1741 agccgcatcg aaatgcatct ggtcgcgcgt cgcgacctgg cggtgcgctg gcagggagga
     1801 gagcgagcgt tcgtccacgg cgagagcatc cataccgaat cgagctacaa gtacacgcgc
     1861 gagcgctttt tttcgctgct cgaagcggcc ggatttcggc atctgcgcag ctgggaagat
     1921 ccgcgcggct ggttcatggt ctgtcttgcc agcgaggagc gacgatgacg gctgcgctgt
     1981 acgatcgatt ccgccgggtg cgcgccgaaa cgctggcgct ggcggcgccg ctgtccgagg
     2041 aagactgctg cgtgcagtcg atgaccgacg cgagcccggt gaaatggcat ctcgcgcaca
     2101 ccagctggtt cttcgagacc ttcatcctcg agcggttcga gcggcccttc catgcgttcc
     2161 agccggcgtt ccggatgttg ttcaactcgt actacaacgg cgtcggcgac aagcatccac
     2221 ggccgcagcg cggcatgatc acgcgccccg ggctggccga ggtgctcgcc taccggcgaa
     2281 atgtcgacga acgtatggcg atggtgatcg gggagggcgc ggcgtcatcc gggcttggcg
     2341 agctggttga actcgggctg cagcacgaac agcagcacca ggaactggtt ctcaccgacc
     2401 ttaagcacct gctttcgatg aatccgctca agcccgccta tgcgcagcgt ggcgcagtgc
     2461 acgctacgct tgcgcccgag caccgctgga tcggcttcga tggcggggtc gtgcatatcg
     2521 gccacgccgg cgacggattc tgcttcgaca acgaattgcc gcgccaccgc cagttcgtcg
     2581 agccctacgc gctgtcgagc cggctggtga ccaacgccga gtacctggct ttcatcgagg
     2641 atggcggcta ccggaatccg gcggtgtggc tcgccgaagg atgggactgg gtgcgaaacg
     2701 gaggaaacgg ggaaagcggg gggagcgggg cgatcgaggc accgctttac tggcacaggg
     2761 ccgaaagcgg ctggcaggaa ttcacgttgc acggcatgca ggcgctcgag cccgaccgtc
     2821 ccgccacgca cctgtctttc tacgaggccg acgcctttgc gcgctgggcc ggcgcgcgcc
     2881 tgcccaccga ggccgaatgg gagcatgcgg cggcatcctg tccgggcgac atcgatccgt
     2941 gcggcatcgc gcagctgttc ggcgtctctt ggcagtggac ctcgtcgagc tattcaccct
     3001 atcccgggtt cacggcgccg cgcggcgcga tcggcgaata caacggcaag ttcatgtgca
     3061 atcagtacgt gctgcgcggt tcgtcgatcg caacaccggc cggccatacg cgcatttcct
     3121 atcgcaattt ctttccggcc tatgcgcgct ggcagttctc aggaatacgt ctggccaggt
     3181 cggcttggaa ggcctgacaa atgcggttca ggtaggcgaa caaccttgtc atgtcgtgcc
     3241 ggcgcaggcg ggagccgatt ctgaagtgct gcgagtgccg gtactgggtt ccggcctcgc
     3301 gggaacggca tggcaagcgt ggtcgcatga atgagcacat ttccgacaca gggc
//
LOCUS       DATLGF010000114         2760 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_98600,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000114 DATLGF010000000
VERSION     DATLGF010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98600"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..1574
                     /gene="ppnN"
                     /locus_tag="VM406_03385"
     CDS             204..1574
                     /gene="ppnN"
                     /locus_tag="VM406_03385"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HVL75034.1"
                     /translation="MNHLVVDALISPEGRLDILSKAEVAKLLDTSQGGLYSTFRSCAL
                     AVLNCGSPLDDGKELLERYKSFDISIIQRERGIKLDIRGAPAHAFVDGKMIKGIHEHL
                     FAVLRDVIYVNDEIIGNPLFDLSTSEGVTDSVFHILRNAGVLRPLTNPNLVVCWGGHS
                     ISREEYDYSKKVGYQLGLRALDICTGCGPGAMKGPMKGAAISHAKQRIGNGRYLGFTE
                     PGIIAAESPNPIVNDLVILPDIEKRLEAFVRAGHGIVVFPGGAGTAEEILYVLGILLH
                     PDNAEIPFPLIFTGPESSQDYFRQIDHFIGATIGPKAQKRYKIIIDDPELVAQEMQAG
                     IKDVREFRKARSDAYYFNWLLKIDAEFQRPFHPNHENMRNLSLNKDQEAHVLAANLRR
                     AFSGVVAGNVKDQGIRAVEQHGRFEIRGDRDIMDPMDALLASFVAQHRMKLPGTAYHP
                     CYRIIK"
     gene            complement(1783..>2760)
                     /locus_tag="VM406_03390"
     CDS             complement(1783..>2760)
                     /locus_tag="VM406_03390"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HVL75035.1"
                     /translation="RIASKVLAEFHNDTTLFSDCLRDFDSFVAEHLREDDDRTPRAIE
                     AVEAAEKYSVLQASATRVLCEVLLPLNVDKRISDFLIAAWPHVLVRAAWRDAQQGGTL
                     LDQYRAVLPELLWSVEPIGAQERQGLIKLLPDLVRRLRAALQSIQLPEDEAKAILDLL
                     VEMHTQVLRAAVASPGRLNLQQLRESFARVGTPWARASWEQSDPPPVRESVVEETFAG
                     IDCEIRVGVAVPASAADRDFLAQTYLLGTRVELRAQAELVPAQLVWISTHRSLYLFKS
                     DAGRFVLYSTAALLEALAEQDVIPLEYAPVFERAVESLLFGAQKLDAPA"
BASE COUNT          522 a          903 c          857 g          478 t
ORIGIN      
        1 ctgctcatgc gcgccgccgc tccagttgcg ctgttgcata tcggggtccg gtctgatggt
       61 gcagcgcgag gccgcgacac cctgtttcga cgcaggccct catgaatcgt tcgaggggaa
      121 atgtctgtca tgcatgacgc cgattgaccc gcggcatgcg cggatacgct aagctcacgg
      181 aaccggacca ctcgccaatt catatgaacc acctcgtcgt cgatgcactg atctcgcccg
      241 aagggcgcct ggacatactc tccaaggcag aagtcgccaa gctgcttgat accagccagg
      301 gcggcctgta ttccaccttc cgtagctgcg cgctggcggt gctcaactgc ggcagtccgc
      361 tcgatgacgg caaggagctg ctcgagcgct acaaatcgtt cgacatcagc atcatccagc
      421 gtgaacgcgg catcaagctc gacatacgcg gcgcccccgc ccacgccttc gtcgacggca
      481 agatgatcaa gggcatccat gaacatctgt tcgcggtgct gcgcgacgtg atctacgtca
      541 atgacgagat catcggcaat ccgttgttcg acctgtccac cagcgagggt gtcaccgact
      601 cggtgttcca catcctgcgc aatgccggcg tactgcgtcc gctgacgaat ccgaatctgg
      661 tcgtctgctg gggtggacat tcaatttcgc gcgaggaata tgactattcg aagaaggtcg
      721 gctatcagct cggcctgcgc gcgctcgaca tctgcaccgg ctgcgggccc ggcgcgatga
      781 aggggccgat gaagggcgca gcgatttcgc atgcgaagca gcgtatcggc aacggccgct
      841 acctggggtt caccgagccc gggatcatcg cggccgagtc gccaaatccg atcgtcaacg
      901 atctcgtgat cctgccggac atcgagaagc gcctcgaagc ctttgtgcgc gccggccacg
      961 gcatcgttgt gtttccaggg ggcgcgggca cggcggagga aatcctgtat gtgctgggca
     1021 tcctgctgca cccggacaat gccgagattc cgtttccgct gatctttacc ggcccggaat
     1081 cctcacagga ctacttccgc cagatcgacc atttcatcgg cgcgacgatc ggcccgaaag
     1141 cgcagaaacg ctacaagatc atcatcgacg atcctgaact ggtcgcgcag gaaatgcagg
     1201 cgggcatcaa ggacgtgcgc gagttccgca aggcacgcag cgacgcctat tacttcaact
     1261 ggctgctcaa gatcgacgcg gagttccagc gcccgttcca tcccaaccac gagaacatgc
     1321 gcaatctgag cctgaacaag gatcaggagg cgcacgtgct cgccgccaac ctgcggcgcg
     1381 cattctcggg tgtcgtggcg gggaacgtga aggaccaggg catccgtgcg gtcgaacagc
     1441 acgggcgttt cgagatccgg ggcgaccgcg acatcatgga cccgatggat gccctgctcg
     1501 cctccttcgt cgcgcagcac cggatgaagc tgccgggcac ggcgtaccat ccttgctacc
     1561 ggatcatcaa gtagcgccga tatcggccaa gacatcggcc catctaccgt cgtgccgctc
     1621 agtcaaggta tcgttgttcc gtcgacgccc cggcgaaagc cggggcccag cgtcttccat
     1681 ggacatgttg acgccgctgg gtgccggctt tcgccgggaa cggccgaacg gtggatgcat
     1741 atctgaacgg cattagccct acaccgctcc aaataccggc acttatgccg gcgcatccag
     1801 tttctgtgcg ccgaaaagca gcgattcgac cgcccgttca aacaccggcg cgtactcgag
     1861 ggggatgacg tcctgctcgg ccagggcctc gagcagcgcc gcggtcgagt acagcacgaa
     1921 cctgccggca tcgctcttga acaggtagag cgagcggtgg gtagagatcc acaccagctg
     1981 cgcgggcacc agttcggcct gcgcgcgcag ctcgacccgg gtgccgagca gataggtctg
     2041 ggcgaggaag tcacggtcgg ccgcgctggc cggcaccgca acgccgacgc gtatctcgca
     2101 gtcgataccg gcgaaggttt cctccacgac ggattcacgt accggcggcg gatcggattg
     2161 ctcccagctc gcgcgtgccc acggtgtgcc gaccctggcg aaactctccc gcaactgctg
     2221 caggttcagg cgaccagggc tggccaccgc cgcacgcaga acctgggtat gcatctccac
     2281 gagaaggtcg agtatcgcct tggcctcgtc ctccggcagc tggatcgact gcagcgcggc
     2341 gcgcagccgg cgcacgaggt cgggcagcag cttgatcagg ccctgccgtt cctgcgcgcc
     2401 gatcggctcc acgctccaca gcaattcagg caggaccgcg cggtactgat cgagcagcgt
     2461 gccgccctgc tgcgcgtcgc gccaggcggc gcgaaccagc acgtgcggcc aggcagcgat
     2521 gaggaaatcc gagatgcgct tgtccacatt cagcggcagc aggacttcac acagcacgcg
     2581 cgtggcgctg gcctgcagca cgctgtactt ctccgccgcc tcgacggcct cgatcgcgcg
     2641 cggcgtgcgg tcgtcgtcct cgcgcaggtg ctcggccacg aaactgtcga aatcgcgcag
     2701 gcagtcggaa aaaagcgtgg tgtcgttatg gaattccgcc agcaccttgg aggcgatgcg
//
LOCUS       DATLGF010000115         3808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_37373,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000115 DATLGF010000000
VERSION     DATLGF010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37373"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..2249
                     /locus_tag="VM406_03395"
     CDS             24..2249
                     /locus_tag="VM406_03395"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HVL75036.1"
                     /translation="MRFVLPLVLVIALVAYVVVPLADSLTLRWFVRDLDTRSQTLAVA
                     LQEPLREYVQQGAEQRIIQMFQRAQRDDRLYAIAFCDATGVLRYHTANYPAALGCREG
                     NTSQSADSLVRLPQGQFHVSQSQLDQDNRTLGKLILVHDMSYIESRSADTRRYLLLLF
                     GVLAVVTSTIAVVAAHLSWRNWISTMKAVLRRGPGTTPRPHTGQADIEPLEGDLRSLL
                     HEYTLERRSNEDATYLWTPEKLRTLLHEELAGDEILVVSNREPYMHARTRHGIEVQRP
                     ASGLVTAVEPVMRACSGTWIAHGSGSADQETVDRNDRVRVPPANPSYTLRRVWLSKEE
                     EQGYYYGFANEGMWPLCHIAHVRPVFRSSDWEQYEKVNRRFADAVVQEARTDDPVVLV
                     QDYHFALLPRMVRNALPKATIITFWHIPWPNPESFGICPWREEILEGLLGSTILGFHT
                     PFHCKNFIETVDRYLETRIEHEASTISFGGELTQVQHYPISIAWPEPDPNAPTVAECR
                     SAIRSELGLSGDHLLALGVDRLDYTKGIIERIQAVERMLELYPGLVGRFSLVQIAAPT
                     RSSLDEYQNFEARVRALVQRVNSRFGGGAYQPVILKAEHHGPELVNRYYRACDVCMVT
                     SLHDGMNLVAKEFIAARDDERGVLLLSQFTGAARELHEALIVNPYHIEQSAEALYRGL
                     TMPEIEQCERMRSMRSLVKDFNIYRWAGRMLLDAARLRQRQRIVMKIRSHSRKSLRRV
                     V"
     gene            2249..3061
                     /gene="otsB"
                     /locus_tag="VM406_03400"
     CDS             2249..3061
                     /gene="otsB"
                     /locus_tag="VM406_03400"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HVL75037.1"
                     /translation="MQHLFSSDEGRRRLAEFAAPGLLCAFDFDGTLTPIISHPGDVRL
                     PEDMRARLARLQEHAPVAIVTGRSLADIAPRLGFTPDLLVGNHGLEGLPGARAAELAE
                     LHLRVCAGWRRQLDTLLAAELPDPGIEIEDKRYSLSVHYRHARAPEAARAELEPLLLG
                     LEPSPRVVAGKCVYNLMPADAGNKGTAMATLIDESAARAAVYIGDDVTDEDVFRVGRA
                     DLLSIRVDAGADDSAASYYLRGVEEVGLLVEQLTERLGAAGARNWLRDRTGV"
     gene            3074..>3808
                     /locus_tag="VM406_03405"
     CDS             3074..>3808
                     /locus_tag="VM406_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HVL75038.1"
                     /translation="MEEGALLGAVDIGGTKVAVSVAGRAGPLARVTEPTVKEGAERAL
                     PDQVIRLLGQACAQAGADAAQLRTIGVASCGPFIRKAGYLAVSTPNICGGLANATDLP
                     NDWVAIPLEAVLRERFERVEIENDGVAALVAERTFGAAVGEPDCVYATWSTGVGFGLC
                     VNGRILRGKHGNAGHGGHMLMSEARDVWCGCGNRGDLEALIAGRSLGEQLGMSTPALF
                     AAARNGDRRALEVAEDAARWFGRGLYN"
BASE COUNT          641 a         1250 c         1287 g          630 t
ORIGIN      
        1 cacctagact acttcgcctt tcgttgcgtt ttgtcctgcc tctcgtactg gtgatcgcgc
       61 tcgtcgccta cgtcgtggtc ccgctggcgg acagcctgac cttgcgctgg ttcgtacgcg
      121 acctcgacac ccgttcgcag acgcttgccg tggccctgca ggaaccgctg cgcgaatatg
      181 tgcagcaggg cgcggagcag cgcatcatcc agatgttcca gcgcgcgcag cgcgacgacc
      241 ggttgtacgc gatcgcgttc tgcgacgcga ccggcgtctt gcgctaccac accgccaatt
      301 accctgccgc gctcggatgc cgcgagggca acacctcgca gtccgccgac tcgctcgtca
      361 ggcttccaca gggacaattt cacgtttcgc aaagccagct ggatcaggac aaccgcacgc
      421 tgggcaagct catactcgtc cacgacatga gttacatcga gtcgcgcagc gccgatacga
      481 ggcgttacct gctcctgctg ttcggcgtgc tggctgttgt cacctcgacg atagccgtcg
      541 tggccgccca tctgagctgg cgcaactgga tttccacaat gaaagcggtg ctgcggcgcg
      601 ggccaggcac taccccgcgc ccgcatacgg ggcaggccga catcgaaccg ctcgaaggcg
      661 atctgcgcag tctgctgcac gagtacacgc tggagcgccg cagcaacgaa gacgcgacct
      721 atctctggac ccccgagaag ttgcgcacgc tgctgcacga ggaactggcc ggagacgaga
      781 tcctggtagt gtcgaaccgc gagccctaca tgcatgcgcg cacccggcac ggcatcgagg
      841 tccagcggcc cgcgagcgga ctggtgaccg ccgtcgagcc ggtgatgcgc gcctgctccg
      901 gtacctggat tgcccacggc agcggttcgg ccgaccagga gacggtcgac cgcaacgacc
      961 gcgtccgggt gccgccggcc aacccgagct acacgctgcg ccgtgtctgg ctatccaagg
     1021 aggaggagca gggctattac tacggctttg ccaacgaggg aatgtggccg ctgtgccata
     1081 tcgcccatgt gcggccggtg ttccgctcgt cggactggga gcagtacgag aaggtcaacc
     1141 ggcgcttcgc cgatgcggtg gtgcaggaag cgcgcaccga cgatccggtg gtgctggtgc
     1201 aggattacca ttttgcgctg ctgccgcgca tggtgcgcaa cgcattgccg aaggcgacca
     1261 tcatcacttt ctggcacatt ccctggccga acccggaatc cttcggcata tgcccgtggc
     1321 gcgaggagat actcgaagga ctgctgggca gcaccattct gggtttccat acgccgttcc
     1381 actgcaagaa tttcatcgag accgtggacc gctatcttga gacgcgcatc gagcacgagg
     1441 cctccaccat ctcctttggc ggcgagctga cccaggtcca gcactatccg atttcgatcg
     1501 catggccgga acccgatccg aacgcgccga cggtcgccga gtgcaggtcg gcgatccgtt
     1561 cggaactcgg cctgtccggc gatcacctgc tggcgctcgg tgtcgaccgc ctcgactata
     1621 cgaaggggat catcgagcgc atccaggcgg tggagcggat gctggagctg taccccggcc
     1681 tggtcgggcg gttctcgctg gtgcagatcg ccgcgccgac ccgctcctcg ctcgacgagt
     1741 accagaactt cgaagcgcgg gtgcgcgcgc tcgtgcagcg cgtcaacagc cgattcggcg
     1801 gcggcgccta ccagccggtg atcctcaagg ccgagcatca cggccccgag ctggtgaatc
     1861 gctactaccg cgcctgcgac gtctgcatgg tcaccagcct gcatgacggg atgaacctcg
     1921 ttgccaagga attcatcgcc gcacgcgacg acgagcgcgg cgtgctgctg ctcagccagt
     1981 tcaccggcgc cgcgcgcgaa ttgcatgaag cgctgatcgt gaatccgtat cacatcgaac
     2041 agagcgccga ggcgttgtat cgcgggctga ccatgccgga gatcgagcag tgcgaacgga
     2101 tgcgcagcat gcgttcgctg gtgaaggact tcaacatcta ccggtgggcc ggccgcatgc
     2161 tgctcgacgc ggcacgcctg cgccagcgcc agcgcatcgt gatgaagatc cgctcgcaca
     2221 gcaggaaatc gctgaggaga gtggtctgat gcagcacctg ttctcgagtg acgagggccg
     2281 gcggcggctg gccgagttcg ccgctcccgg cctgttgtgc gcgttcgatt tcgacggcac
     2341 gctgaccccg atcatctccc accccggcga cgtgcgcctg cccgaggaca tgcgtgcgcg
     2401 cctggcgcgg ctgcaggaac atgcgccagt cgccatcgtc accgggcgtt cgctggcgga
     2461 catcgctcca cgcctgggat tcacgcccga tcttctggtc ggcaatcacg gactggaagg
     2521 cctgcccggc gcgcgcgcgg ccgagctggc ggaactgcac ctgcgcgtgt gtgccggatg
     2581 gcggcgccag ctcgatacgc tgcttgcggc cgaactgccg gatccgggca tcgagatcga
     2641 ggacaagcgc tactcgctgt ccgtgcatta ccggcatgcg cgcgcgccgg aggcggcccg
     2701 ggccgagctt gagccgctgc tgctcggcct ggagccgtcg ccgcgcgtcg ttgccggcaa
     2761 atgcgtgtac aacctgatgc cggccgacgc gggcaacaag ggcacggcaa tggcaacgct
     2821 gatcgacgag agcgctgcgc gcgcagcggt ctacatcggc gacgacgtga ccgacgaaga
     2881 tgtgttccgc gtcggtcgtg ccgacctgct gtcgatacgg gttgacgccg gcgccgacga
     2941 cagcgcagcg tcgtattatc tcaggggggt ggaggaagtc ggtctgctag tcgagcagct
     3001 gaccgaacgg ctcggcgccg ccggtgcgcg caactggctg cgcgatagaa ccggcgtata
     3061 gaaggaggcg aggatggagg agggcgctct gctcggcgcg gtggacatcg gcggcaccaa
     3121 ggtggcggtc agcgtggctg gaagggccgg gccgctggcg cgcgtgaccg agccgacggt
     3181 caaagagggg gccgagcgcg ctttgccgga tcaggtcatc cgcctgctcg ggcaggcctg
     3241 cgcccaggcc ggtgcggatg cggcgcagct gcgcaccatc ggggtcgcat cctgcggtcc
     3301 gttcatacgc aaagccgggt acctcgccgt gtccacgccg aacatttgcg gcgggctcgc
     3361 caacgccacc gatctgccca acgactgggt cgcgattccg ctggaggcgg tactgcgcga
     3421 acgattcgaa cgcgtcgaaa tcgagaacga cggcgtcgct gcactggtcg ccgagcgcac
     3481 cttcggtgcg gcggtcggcg agcccgactg cgtgtacgcg acctggagca caggcgtcgg
     3541 cttcggcctg tgcgtcaacg gccgtatcct gcgcggcaag cacggcaatg ccggccatgg
     3601 cgggcacatg ctgatgagcg aggcgcgcga cgtatggtgc ggatgcggca accgcggaga
     3661 tctcgaggcc ttgattgcgg ggcgcagtct cggcgagcaa cttggcatga gcacgccggc
     3721 gctgttcgcc gccgcaagaa acggcgaccg gcgcgcgctg gaagtcgccg aagatgccgc
     3781 gcgctggttc ggccgtggct tgtacaac
//
LOCUS       DATLGF010000116        13348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_2758,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000116 DATLGF010000000
VERSION     DATLGF010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 13348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13348
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2758"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1732
                     /gene="pepN"
                     /locus_tag="VM406_03410"
     CDS             <1..1732
                     /gene="pepN"
                     /locus_tag="VM406_03410"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HVL75039.1"
                     /translation="DWFQLSLKEGLTVFRDQEFSADMVGTPSGRAVKRIEDVRVLRQA
                     QFPEDAGPMAHPVRPDSYVEINNFYTVTIYEKGSEVVRMYQTLLGREGFRRGMDLYFQ
                     RHDGQAVTCDDFRAAMADAGKRDLSQFELWYSQAGTPHLKVETRYDETSQTYMVTLTQ
                     SCPPTPGQKEKLPFHIPVAIGLLDSGGRDMPLVLDKIEGGDDEPVTTMVLELTEPSQT
                     FHFVDIHECPVPSVLRNFSAPVILDIDYTDAELAFLMANDSDAFNRWEAGQRLAMRRM
                     LALVAIVQSGRTADDLIFVEGVLTDAFRSTLIDTRLDPAFVDVALTLPSEVVIAEQME
                     QIDPQAIHTVRRFMRRSLAEALRVDWEVAYQANRTPGAYSPDPASVAKRALKNLALTY
                     LAELDDEAAHELAQTQYDNANNMTDRMAALSALVHGTAPGREAMLAHFYEEFEHEALV
                     VDKWFALQATSRTTDVAAVRRLMEHPAFTLKNPNRARSLIFSFCNGNHAQFHAPDGSG
                     YAFWAEQVIALNALNPQVAARLARSLDRWRKYVPALQDKMRAALERVASSKGLSKDVL
                     EVVTKSLSNN"
     gene            1765..2769
                     /locus_tag="VM406_03415"
     CDS             1765..2769
                     /locus_tag="VM406_03415"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HVL75040.1"
                     /translation="MKRVSLTQYLVEEQRLHNTIPAELRLLIEVVARACKAISNSVGK
                     GALGEVLGSADSENVQGEVQKKLDVISNELLLQANEWGGHLAAMASEEMETIHPIPHR
                     YPKGEYLLLFDPLDGSSNIDVNVSIGTIFSVLKAPEGMGEPTEEAFLQCGAKQVAAGY
                     AVYGPQTVLVLTTGNGVQCFTLDREMGSWVLTQRDMQIPAETKEYAINASNARHWYPP
                     VKRYIDELQAGETGPRGKNFNMRWIASMVADVHRILNRGGIFMYPADAREPDKPGKLR
                     LMYEANPMAFIVEQAGGAATNGRQRIMDIQPKKLHERVAVFLGSKSEVERVTSYHVDA
                     "
     gene            2837..2912
                     /locus_tag="VM406_03420"
     tRNA            2837..2912
                     /locus_tag="VM406_03420"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2870..2872,aa:Thr,seq:cgt)
     gene            3236..4456
                     /locus_tag="VM406_03425"
     CDS             3236..4456
                     /locus_tag="VM406_03425"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVL75041.1"
                     /translation="MTFLHAPTLALAIAIVQSALTAILLFFLFTRKTYPGFARLISCQ
                     VFWCAAYFLILGRGVLPDAISIVLANYLIILGLGILYEGLAEIADSPSSGRTRAMIHL
                     GGLACIALMSWHLYIEPNFNARVAELNVYRIAMSGLCVALVLRHRASPQFRAVLPLMM
                     VLFGVSIVSSAGRAFLVMQAAPIESVSNDLMFHLHMLVDLFLFVVFGFIVLLLSTVRT
                     EQELEVARRQAEHASRADPLTGLWNRLHFESAGRFEEARAKRYKLPLSVMVFDIDHFK
                     DVNDRNGHLTGDSVIRAVASVATDTLRSSDLIFRWGGEEFVVLLPVDIHAAADAAEKL
                     RLAIEGTEIPGCGKVTVSVGVAQMDSDKDLMECLQRADKQLYRAKEAGRNRTMVQAGT
                     GGSGTAAVAQEGAL"
     gene            complement(5001..7586)
                     /locus_tag="VM406_03430"
     CDS             complement(5001..7586)
                     /locus_tag="VM406_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVL75042.1"
                     /translation="MSERVSREQQRLKALEALEILDTPSEPMFDRLTQLAATLCRTPI
                     ASISLIDGNRQWFKSRVGLDLTEVARTVSICTYTLLQGRPVVVEDTLNDPNFRSNAFV
                     VGPPHVRFYAGIPLITSDGQTVGTLSVMGRQPRRLSTTKLELLQLLADQIIAQMELRS
                     QRAALDRALGAHEELNAKLAHQAEHLRAAQRIAQVGSWEMRLPDRQLRLSEETFRIFG
                     VGYRDEAVEFAAFMSSVHVDDRAGLELALERALAADGTLDHAHRIVRPGGEIRYVHQR
                     GELRTGADRAQVLAGTVQDITQEEESKAALQLLSACVSRVSDTVMITEACPCAEPGPR
                     IVFVNEAFEDITGYPVREVIGRSPRFLQGPRTQRSQLDRIRSALERNERVSCEIINYR
                     KDGREFWVEIDIAPVVIGGQVTHFVSVQRDITERKASEQKIEQLAFFDPLTQLPNRRL
                     LMDRLDHAIKTTLRKTCCGAVLFLDLDNFKSINDTLGHDKGDRLLIHVAQRLNKVIRA
                     SNTVARIGGDEFVILLEDLSHNRMEAAAQAEYVAEKVLGCVQEPVDIDGYEHACTTSI
                     GVILFNNEDDNGDELLKRADVAMYQAKAAGRNVIRFFDQGMQTLVNRRVMLERDMRSS
                     LRRGDFILHYQPQVDADGTVVGIEALVRWNHPREGLLYPSAFIPLAEESRLIVQLGQW
                     VLEDACRNLASRQAGPGSTAPRMSVNVSAQQFHHPEFLPRTLQAIEAARVDPRNLKLE
                     LSESILVENFESTAEKMKTLKKLGVRFSLDDFGTGYSSLSYLKRLPVDEVKIDRAFVR
                     DILHDPDDAAIVRTIVSLCDILGFDVIAEGVETEGQRRFLAEIGCRVYQGNLFSPPRP
                     LVPSH"
     gene            complement(7788..8606)
                     /locus_tag="VM406_03435"
     CDS             complement(7788..8606)
                     /locus_tag="VM406_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HVL75043.1"
                     /translation="MKVRSMKEDDPLEDFAHREITLEGKTRTVHVAGSGAAVIVMAEM
                     PGISPQVARFARWVRDAGFTVYMPSLFGRDGAVPDAEEGAEVFRRACISAEFRALGGG
                     QSSPVTQWLRALARLAHGECGGPGVGAVGMCFTGNFALTMMLEPAMLAPVLCQPSLPF
                     DAPAALEIAPDELAAVARRLEREDLTALAYRFEGDRFCRAQRFAAYAEALGERFVARV
                     LPDSAASTDVAPFFAQHVRSPHSVVTAHLIDAVGQPTLAARDEILAFFRRRLAA"
     gene            8709..9725
                     /locus_tag="VM406_03440"
     CDS             8709..9725
                     /locus_tag="VM406_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVL75044.1"
                     /translation="MNDFTVLILPGANASSVATTLDVLAAAAATASRAGASAPRWRVL
                     CAGGASAPLSNGMSVKGAALPARVRSEHSVWIVPGLGLTHPKALAQRFTEPDALRAIA
                     ALRAHARAGGAVAASCSAVFLLQAAGLLAGRKVTTSWWLAPLLHRLAPGCTVDADRMV
                     IEDGPLVTAGAAMAQTDLMLHLVRTRFGVALADAVARVLLIDARAAQAPFVVPEMMAM
                     GNELVARITARVEAGLPHAPCIEELAMEFAMSARTLARHVKAATGRSPLALVQSVRLN
                     KARALLENSKLSVDEVALRVGYEDATALRRLMRKATGATPRQFRANGREGRPAPLRSS
                     ASPA"
     gene            9833..10900
                     /locus_tag="VM406_03445"
     CDS             9833..10900
                     /locus_tag="VM406_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HVL75045.1"
                     /translation="MVWTFIGGLVALVAGAELLVRGASRLALSLGISPLVVGLTIVAF
                     GTSAPEVAVSVGAVLEGQINIAVGNVVGSNIFNVLFILGLSALITPLVVHSQIIRQEV
                     PIMIGASLLLLVQGLDGVLGLFDAALLAALLIAYTVFLILQSRREAAGEPDAGSQSTS
                     VAGKWTSHWAVQLLLIAAGLGLLVLGSQWLVQSAVTFARLIGVSDLVIGLTIVAAGTS
                     MPEVATSVMAALRKERDIAIGNVVGSCTFNILGCLGIAGLASAQGLSLAPAVLNFDIW
                     VMLAVSFACLPVFISGREIARWEGGVFLAYYAAYATYLIMAAQSHERLAQFSAVMMSF
                     VLPITVITLTVVLLRPAVRRG"
     gene            11103..11501
                     /locus_tag="VM406_03450"
     CDS             11103..11501
                     /locus_tag="VM406_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVL75046.1"
                     /translation="MYAYEQVRKYREAVLSGNLAAVLELFEPAATVSDPIAGHGSAQE
                     FHTCLFRKRRRAIARLRNVLDGLDKNVIGLQFSYTWVLDDGNTLTAEGVTLFELAPET
                     GRFTKLTLIYDASDLRRHLPRADFEGLALN"
     gene            11664..12824
                     /locus_tag="VM406_03455"
     CDS             11664..12824
                     /locus_tag="VM406_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HVL75047.1"
                     /translation="MKTMFRNLALCAPVALLAVACGGGSSDTSHGTLGVYLTDAPACG
                     FNEVNVTISKVRVHAAENAGENASGWHEIVLEPARQFDLLKLTNGVLAELGEVELPAG
                     RYTQMRLVLAPNTGAAPLANSVVPVGGSPVALATPSAIQTGIKLVGGFDIAGGDRTEL
                     ALDFDACKSVVTRGHGGHLLKPVISIVPMASSGAISGVLDPSITAADNPMVSAQVDGV
                     IVKSTVPDTTGAFSLAPLPAGNYTVVVTADARASDVITSVPVTVSKDTPLSTAAAPLT
                     LQPSATATVSGKLTPADAQALVSATQSFAAGPRVTIKYRNADVVTGDYSMTLPTAAPR
                     LGQFGALPLTLTAQPAIAGKYSVEASAAGYQPRTQAADVSTGDVSTDFSLAP"
     gene            13057..>13348
                     /locus_tag="VM406_03460"
     CDS             13057..>13348
                     /locus_tag="VM406_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HVL75048.1"
                     /translation="MPLLAPSRTSDDLGKLLLRVTLAFLILLHGIAKVLNGVDPIMGM
                     LARQGFPPALAYLVYLGEVIAPVLIIIGIWTRPAALIIAINMVVAVWLVHMRE"
BASE COUNT         2300 a         4306 c         4305 g         2437 t
ORIGIN      
        1 cgactggttc cagctgtcgc tgaaggaagg cctgacggtg ttccgtgacc aggagttctc
       61 cgccgacatg gtcggcaccc caagcgggcg cgctgtcaag cgcatcgagg acgtgcgcgt
      121 gctgcgccag gcccagtttc ccgaagacgc gggcccgatg gcgcacccgg tgcgtcccga
      181 ttcctacgtc gagatcaaca atttctacac ggtcacgatc tacgagaagg gctcggaagt
      241 ggtcaggatg tatcagaccc tgctcggccg cgaaggcttc cgccgcggga tggacctgta
      301 cttccagcgc catgacggac aggcggtcac gtgcgacgac ttccgcgccg cgatggccga
      361 cgccggcaag cgcgacctgt cgcagttcga gctctggtac agccaggccg gcacgccgca
      421 tttaaaggtc gagacgcgct acgacgagac ttcgcagacc tacatggtga cgctcacgca
      481 gagctgccca cccacgcccg gacagaagga aaaactgccg ttccacattc cggtcgccat
      541 cggcctgctc gattccggcg ggcgcgacat gccgctggtg ctggacaaga tcgaaggcgg
      601 cgacgatgaa ccggtcacga cgatggtgct ggagctgacc gagccgagcc agaccttcca
      661 tttcgtcgac atccacgaat gtccggtgcc ctcggtgctg cgcaacttct ccgcaccggt
      721 catcctcgac atcgactaca ccgacgccga actcgcattc ctgatggcca acgacagcga
      781 cgcgttcaac cgctgggaag ccggccagcg cctggcgatg cgccgcatgc tggcgctggt
      841 cgcaatcgtg cagagcggac gtaccgcgga cgatctgata tttgtcgaag gcgtgctcac
      901 cgatgcattc cgctcgacgc tgatcgacac ccggctcgat cccgccttcg tcgacgttgc
      961 gctcacgctg ccgtcggaag tggtcatcgc cgagcagatg gagcagatcg atccgcaggc
     1021 gatccacacg gtgcgccgct tcatgcgccg ctcgctcgcc gaggcgctgc gggtggattg
     1081 ggaagttgcc tatcaggcca accgtacacc cggcgcgtac agtccggatc ctgcctccgt
     1141 cgccaagcgt gcgctgaaga atctggcgct gacctacctg gccgagctgg acgatgaagc
     1201 cgcgcacgaa ctggcgcaga cccagtacga caacgccaac aacatgaccg accggatggc
     1261 cgcgctgtcc gcgctggtcc acggtaccgc gccgggtcgc gaagccatgc tcgcccactt
     1321 ctatgaagag ttcgagcatg aagcgctggt ggtcgacaag tggtttgcct tgcaggccac
     1381 ctcgcgcacc accgatgtgg cggccgtgcg ccggctgatg gaacatccgg cgtttacgct
     1441 gaagaatccg aaccgcgcgc gcagcctgat cttcagcttc tgcaacggca atcatgcgca
     1501 gttccacgcg ccggacggca gcggctatgc gttctgggcc gaacaggtga tcgcgctcaa
     1561 tgcgctgaac ccgcaggtcg ccgcccgcct tgcccgctcg ctggaccgct ggcgcaagta
     1621 tgtgcctgcg ctgcaggaca agatgcgcgc cgcgctggag cgggttgcct cctccaaggg
     1681 gctctcgaag gacgtgctcg aagtcgtcac caagtcgctc tcgaataatt gagtcataga
     1741 taacaaccac cacccgaacc caccatgaaa cgagtcagcc tcacccaata cctggtcgaa
     1801 gaacagcgcc tgcacaacac gattcccgcc gagctgcgcc tgttgatcga agtcgtcgca
     1861 cgcgcctgca aggccatcag caactcggtc ggcaagggcg cgctcggcga ggtgctcggc
     1921 agcgccgaca gcgagaacgt gcagggcgag gtgcagaaga aactggacgt gatcagcaac
     1981 gagctgctgc tgcaggccaa cgagtggggc ggccacctcg ccgcgatggc gtccgaggaa
     2041 atggagacca tccacccgat cccgcaccgc tatccgaagg gcgaatacct gctgctgttc
     2101 gatccgttgg acggctcgtc gaacatcgac gtcaacgtct cgatcggcac catcttctcg
     2161 gtgctgaagg cccccgaagg catgggggaa ccgacagagg aagccttcct gcagtgcggc
     2221 gcaaaacagg tcgccgccgg ctacgcggtc tacggcccgc agacggtgct ggtgctcacc
     2281 accggcaacg gcgtgcaatg cttcacgctg gaccgcgaga tgggctcctg ggtgctcacg
     2341 cagcgcgaca tgcagattcc ggccgagacc aaggaatatg cgatcaacgc ctccaacgcg
     2401 cgccactggt acccgccggt caagcgctac atcgacgaac ttcaggccgg cgagaccggc
     2461 ccgcgtggca agaacttcaa catgcgctgg atcgcctcga tggtcgccga cgtccatcgc
     2521 atcctgaacc gcggcggcat cttcatgtac ccggccgatg cccgcgaacc cgacaagccc
     2581 ggcaagctgc gcctgatgta cgaagccaat ccgatggcct tcatcgtcga acaggccggc
     2641 ggcgccgcca ccaacggcag gcagcgcatc atggacatcc agccgaaaaa gctgcacgaa
     2701 cgcgtggcgg tcttcctcgg ctctaagagc gaggtcgagc gggtcacgag ctaccacgtc
     2761 gatgcttgac tcaggccaat tcccgcagcg gcggggctag cgggaaagcg gggtttattg
     2821 ttagaatcgc cgtttcgctg atgtagctca gttggtagag caactgattc gtaatcagta
     2881 ggtcggaggt tcgactcctc tcatcagcac cagaaaatca aggggttagc gccgaaaggt
     2941 gctagcccct ttctgttttg cctgaaatcg tgcatcggac atcttcgtct gcttgccttg
     3001 ccttctacgc ctttcgccag gttctccgcc gtccaggctc aatccgcagg cgtgcgcccg
     3061 ctccgacgga gtagctcgat tcacgcggcg cacgagtttc ggatccgcgc gcgtcgccct
     3121 gacatccccg tagtgggtac aacgcccctg gtggaccgat tgcgtccgct gtgcacgcaa
     3181 ttgccggggc gcggatggga gcgtatagtt gctcaccata aatattcgcg cctagatgac
     3241 tttcctccac gctccgacgc tcgcgctcgc gatcgccatt gtccagtcgg cgctcacggc
     3301 catcctcctg tttttccttt tcacgcgtaa aacctatcct ggtttcgcgc gcctgatcag
     3361 ctgccaggtt ttctggtgcg cagcctattt cctcatcctc ggtcgcgggg tcttgccgga
     3421 cgcgatatcc atcgttctcg ccaactactt gatcatcctc ggcctcggca tcctttacga
     3481 gggcctggcc gagattgccg acagtccgag cagcgggcgc acgcgcgcca tgatccatct
     3541 tggcgggctg gcctgcatcg cattgatgtc gtggcatttg tacatcgagc cgaacttcaa
     3601 tgcgcgggtg gcggaattga atgtctatcg catcgccatg tcgggcttgt gcgtcgcgct
     3661 cgtgctgcgc catcgcgctt cgccgcagtt ccgtgccgtt ctccccttga tgatggtgct
     3721 gttcggcgtt tccatcgtct cgtcagccgg gcgcgctttt ctggtgatgc aggccgcgcc
     3781 gatcgaaagc gtgagtaacg atctcatgtt ccatctgcac atgctggtgg atctgttcct
     3841 gttcgtcgtg ttcgggttca tcgtgctgct gttgtccacc gtgcgcaccg agcaggaact
     3901 ggaggtggcg cgtcgacagg ccgagcacgc gtccagggcg gatccgctca ccggcttgtg
     3961 gaatcgcctg catttcgaga gtgcgggccg attcgaagag gcgcgcgcaa agcgctacaa
     4021 gctgccgctg tcggtgatgg tgttcgacat cgaccatttc aaggacgtca acgaccgcaa
     4081 cggccatctg acgggcgaca gcgttatccg ggctgttgca tcagtggcca ccgacacatt
     4141 gcgctccagc gacctgatct tccgctgggg aggcgaggag ttcgtggtgc tgctgccggt
     4201 cgatatccat gcggccgcgg acgcagccga gaagctgcga ttggcgatcg aaggaaccga
     4261 gattccaggc tgcggcaagg tgaccgtgtc ggtgggcgtg gcccagatgg attccgacaa
     4321 ggacctgatg gaatgcctgc agcgagcgga caaacagttg tacagggcaa aggaagcagg
     4381 gcgaaatcgc accatggttc aggcggggac gggcggcagc ggtaccgctg cggtggccca
     4441 ggaaggcgca ctgtaaccgc aatgttcagc gcctctcacc agcaccagga caagcaaggg
     4501 gttgggcgac gcgagcccct tgcgcgcgca gcttccgcgc acccagcttc gcatgggtcg
     4561 aaacgaacac tttcgacgcg gaacgccggc acgtccgggc gatggcctgc agctctgcag
     4621 gcgaccaggc gcacgacgtc actggctaca cccacgagca gctgaccgac gaccttctta
     4681 accgctacgc acgcttccgc catacccggc ggctttcgtg aacgagagtg aatcgccctg
     4741 gaattcctgg acactcgcca gcctctcgaa gtactgctgc tcggggtttt ttcggcgagt
     4801 gccggttgta gtacccgtgg cgacgttccg gctaagactg cctgggcaaa ccatgcggca
     4861 cggcaggccg gcacccaatt tcagcctgcc gcgcttcgtg gcgcagttgc gcaaaccgcg
     4921 gcttgggtgt cgatgccaag cttatgcaag cgcatgaagt catacgtctc gatcgagcag
     4981 tctccccagc gttatatcgc tcaatgcgat gggaccagtg gtcgcggcgg gctgaacaga
     5041 ttgccctgat agacccggca gccgatctcg gcgaggaagc ggcgctgccc ttcggtttcg
     5101 acgccctcgg cgatcacgtc gaagccgaga atgtcgcaca gcgacacgat ggtcctgacg
     5161 atcgccgcgt catccgggtc gtggagaatg tcgcgcacga acgcacggtc gatcttcact
     5221 tcgtcgaccg gcaggcgctt caggtaggac agcgatgaat agccggtgcc gaaatcgtcg
     5281 agcgaaaagc gcacgccgag cttcttcaag gttttcattt tttccgccgt cgactcgaag
     5341 ttctcgacga ggatgctttc cgacagttcc agcttgagat tgcgtggatc gacgcgggcg
     5401 gcttcaatgg cctgcagtgt acgaggcagg aactccgggt ggtggaattg ctgcgcgctg
     5461 acgttgaccg acatgcgcgg cgccgttgac ccggggccag cctggcgcga cgcgaggttg
     5521 cgacaggcgt cctccagcac ccattgcccg agctggacga tcaggcggct ttcttcggca
     5581 agcggaatga acgcggacgg gtagagcaga ccctcgcgcg gatgattcca cctgaccagg
     5641 gcttcgatgc cgactaccgt accgtcggcg tcgacctgcg gctgatagtg caggatgaag
     5701 tcgcctcgac gcaggctgct gcgcatatcg cgttcgagca tgacccggcg gttgacgagc
     5761 gtctgcatgc cctggtcgaa gaagcggatc acgttgcgtc cggccgcctt ggcctggtac
     5821 atcgcgacgt cggcgcgctt gagcagctcg tcgccgttgt catcctcgtt gttgaagagg
     5881 atgacgccga tactggtggt gcacgcatgt tcgtagccgt cgatgtcgac cggttcctgc
     5941 acgcagccga ggaccttttc ggcgacatat tccgcctgtg cggcagcctc catccggttg
     6001 tggctcagat cttccaggag gatgacgaat tcgtcgccac cgatgcgcgc gaccgtattg
     6061 ctggccctga tcaccttgtt caggcgctgc gcgacgtgta tcagcaggcg gtcgcccttg
     6121 tcgtgtccga gcgtgtcgtt gatgctcttg aagttgtcca ggtccaggaa tagcacggcg
     6181 ccgcagcacg tcttgcgcaa ggtcgtcttg atcgcatggt cgagccggtc catcagcagg
     6241 cggcggttcg gcagctgggt cagcggatcg aagaacgcga gctgttcgat cttctgttca
     6301 ctggccttgc gctcggtgat atcgcgctgg accgaaacga agtgggtgac ctgaccgccg
     6361 atgacgaccg gggcgatatc gatttccacc cagaactctc tcccgtcctt gcggtaattg
     6421 atgatttcgc aagatacccg ttcattcctt tccagcgcgc tgcggatccg gtccagctgg
     6481 gagcgctggg tcctgggacc ttgcaggaag cgcggcgagc gtccgatcac ctcgcgtacc
     6541 ggatagccgg taatgtcctc gaacgcttca ttgacgaaga cgatgcgcgg cccgggctcg
     6601 gcgcaggggc aggcctccgt gatcatcacc gtgtcgctca cgcgcgatac gcaggcggag
     6661 agcagttgga gcgcggcttt cgactcttcc tcctgggtga tgtcctgtac cgttcccgcg
     6721 agcacctggg cacgatccgc acctgtgcgc agttctccac gttgatggac ataccggatc
     6781 tcgccgcccg gccgtacgat ccgatgggcg tggtcgagcg tcccgtccgc cgcgagcgcg
     6841 cgctcaagcg ccagttccag cccggcgcgg tcgtcgacat gcacggacga catgaaggcg
     6901 gcgaattcga ccgcctcgtc gcggtacccg acgccgaaga tacgaaacgt ctcctcggac
     6961 agacgcagtt gccggtcagg cagccgcatc tcccagcttc ccacttgggc gatgcgctgg
     7021 gccgcgcgca agtgttcggc ctgatgggcg agtttcgcgt tcagctcctc gtgggcgccg
     7081 agggcgcggt ccagcgcggc gcgctggctg cgcagctcca tctgcgcaat gatctggtcg
     7141 gcaagcagct gcagcagttc cagcttggtc gtgctcaggc gccgcggctg gcggcccatc
     7201 acggacaggg tgcccacagt ttgtccgtca gaggtgatca gcggaatccc cgcgtagaag
     7261 cgcacatggg gcgggccgac cacaaatgcg ttgctgcgga aattcggatc gttcagcgtg
     7321 tcttcgacca cgaccggtcg gccctgaagc agggtgtagg tgcagatcga gacggtccgg
     7381 gcaacctcgg tcaggtccag gccgactctc gatttgaacc attgccggtt accgtcgatc
     7441 agggaaatcg atgcgatcgg cgtgcggcag agcgtggcgg cgagttgcgt gagccgatcg
     7501 aacatcggct cggaaggcgt atcgagaatc tcaagcgctt ctagtgcctt gagcctctgc
     7561 tgctccctgg atactcgttc ggacatgctc gctctccgat cgcggcaacg ggttctgacc
     7621 ctgacctccc gattgtgaga cctggttgtc ccgttgttcc tgctcgagtc aagatttctg
     7681 tcgcaagcat attccgcgcc ggccgcggcg gcagcgcgat gaatcaaatc gaaggcgcat
     7741 ggcgggcaag aacggttttc atgcccgcat gcggccgcgc aacacgttta tgccgccagc
     7801 cgccgcctga agaacgcgag gatctcgtca cgggcggcga gggtgggctg gccgaccgcg
     7861 tcgatcaggt gggccgtcac tacgctatgc gggctgcgca catgctgcgc gaagaacggc
     7921 gccacatcgg tgctggcggc gctgtcgggg aggacgcgtg cgacgaagcg ttcgccgagc
     7981 gcctcggcgt aggcggcgaa gcgctgcgcg cggcagaagc ggtccccttc gaagcgatag
     8041 gccagcgccg tgagatcttc ccgctcgaga cgccgggcca ccgcggccag ttcgtcgggc
     8101 gcgatctcca gcgctgccgg agcgtcgaac ggcagcgagg gctggcacag caccggagcg
     8161 agcatcgccg gctcgagcat catcgtgagc gcgaagttac cggtgaagca catgccgacc
     8221 gcgcccacac cgggtccgcc gcattcaccg tgcgcgagcc gggcaagcgc gcgcagccac
     8281 tgcgtgaccg ggctcgactg gccgccgccg agcgcgcgaa actcggcgct gatgcaggcg
     8341 cgccggaaca cctcggcgcc ttcctcggcg tccggcaccg ccccgtcgcg cccgaacagc
     8401 gacggcatgt acacggtgaa gccggcgtcg cgcacccagc gggcgaagcg ggcgacctgc
     8461 ggactgattc cgggcatctc ggccatgacg atcacggccg cgccgctgcc ggctacgtgg
     8521 acggtcctgg ttttgccctc gagcgtgatc tcccgatgcg cgaagtcctc gagcggatcg
     8581 tcctctttca tgctgcgcac tttcatctga agccctcctg ttgtgggttg acgggagtca
     8641 taagtacacc tcaccgcggc ctcgtccggg agtgtcggaa tcgacatctt tcgtgctaga
     8701 tttgccacat gaatgatttc accgttctga tccttccggg cgcgaatgcg tccagcgtcg
     8761 cgaccacgct cgacgtgctg gccgcggcgg cggcgacggc ctcgcgcgcc ggcgcgagcg
     8821 cgccgcgctg gcgcgtgctc tgcgcgggtg gagcgagcgc gcccttgtcg aacgggatgt
     8881 cggtgaaggg agcggcgctt cctgcccgcg tgcggtccga gcattcggtc tggatcgtgc
     8941 ccggcctagg gctgacacat ccgaaggcgc tcgcgcagcg ctttacggag ccggacgcgt
     9001 tgcgggcgat cgcggcgctg cgcgcgcacg cgcgcgccgg cggcgcggtg gcggcctcgt
     9061 gctcggccgt gttcctgctg caggccgccg ggctgctggc aggaaggaag gtgaccacct
     9121 catggtggct ggcgccgctg ctgcacaggc tcgcgccggg ttgcacggtg gatgcggacc
     9181 gcatggtgat cgaagacggc ccgctggtga ccgccggtgc ggcgatggcg cagaccgacc
     9241 tgatgctgca ccttgtgaga acgcgcttcg gcgtggcgct ggccgatgcg gtcgcgcggg
     9301 tgctgctgat tgatgcgcgc gccgcgcagg cgccgttcgt cgtgccggaa atgatggcaa
     9361 tgggtaacga gctggttgcc cggatcactg cgcgcgtgga ggcgggcttg ccgcatgcgc
     9421 cctgcatcga ggagcttgcc atggaattcg cgatgtccgc acgcacgctg gcgcggcacg
     9481 tgaaggcagc caccgggcgc agtccgctgg cgcttgtgca gagtgtgcgc ttgaacaagg
     9541 cgcgcgcgct gctggagaac agcaagctgt cagtcgacga agtggcgctg cgcgtcggct
     9601 atgaggacgc gaccgcgctg cggcgcttga tgcgaaaggc aaccggcgcc acgccgcggc
     9661 agttccgcgc gaacggacgc gagggccgcc cggcaccgct gcgcagttca gcctcgcccg
     9721 cataatgcgg ccgccggaga cgcggcttct tgcgtcgagt ccaccttgaa caaagcgcag
     9781 ttgcgcgccc ttgctcataa tggcgcgacg actgacgaga ggagagggcg tgatggtgtg
     9841 gacgttcata ggcggcctgg tggcactggt cgcaggcgcg gaactgctgg tgcggggcgc
     9901 atcccggctc gctctgtctc tgggaatctc tccgctcgtg gtcggcctga cgatcgttgc
     9961 gttcggcacc agcgccccgg aagtcgcggt ttcggtcggc gcggtcctcg aaggacagat
    10021 caatatcgca gtcggcaacg tggtcggcag caacatattc aacgtgctgt tcatcctcgg
    10081 gctgtctgcg ctgatcacgc cgctggtcgt ccacagccag atcatccgcc aggaagtgcc
    10141 gatcatgatc ggcgcctcgc tgctgctgct cgtgcaggga ctggacggtg tgctcggcct
    10201 gttcgacgcc gctctgctgg ccgctttgct gatcgcctac acggtattcc tgatcctgca
    10261 atcccgtcgc gaggcagcgg gcgagccgga cgcagggtcg cagagcacaa gcgtggcggg
    10321 taaatggacg tcccactggg ccgtgcagct gctgctcatt gccgcgggac tggggctgct
    10381 cgtgctcggc tcgcagtggc tggtgcagtc ggccgtcacc ttcgcgcggc ttatcggcgt
    10441 gagcgacctg gtgatcggcc tgacgatcgt ggccgccggg acctcgatgc ccgaagtggc
    10501 gacctccgtc atggcggccc tgcgcaagga gcgcgatatc gcgatcggca acgtggtggg
    10561 cagctgcacc ttcaacatcc tgggctgcct cggcatcgcg ggactggcga gcgcgcaggg
    10621 gctcagtctt gcgccggcag tgctgaactt cgatatctgg gtgatgcttg ccgtctcgtt
    10681 cgcctgcctg ccggtattca tttcgggccg tgaaatcgcg cgctgggaag gaggggtgtt
    10741 tctcgcctac tacgccgcct acgcgaccta cctgatcatg gccgcgcaaa gccacgaacg
    10801 gcttgcccag ttcagcgcgg tgatgatgtc ctttgtcctg cccattactg tgatcacgct
    10861 cactgtcgtg ctgctgcggc cggccgtccg gcgcggctga gtccgcgaag aacgcgaggc
    10921 gcaggcgccg actcccgcga gcggccacgc ctcccccgcc ggcacgggcg gaggtgaacc
    10981 gccatgcttg atgtaagtca agccgttgag atatcccaaa gttatgggat tgctgcgccg
    11041 cagcagggtg catatccttt cagcaatcag ggttagtcgg cctcatttca agcgggcaaa
    11101 gcatgtatgc atatgaacag gtgcgcaaat accgggaagc ggttctttcc gggaacctgg
    11161 ctgcagtact cgaattattc gagccggcgg cgacggtgag cgatccgatt gccggccatg
    11221 gttcggcgca agagtttcat acctgtttgt tccgcaagcg ccggcgcgcg atcgcacgcc
    11281 tgcgcaatgt actcgatggc ctcgacaaga acgtgatcgg actccagttc agctacacct
    11341 gggtgctcga cgatggcaat acgctgactg cggaaggcgt cacactgttt gaactggcac
    11401 ccgaaaccgg acgtttcacg aagctcacgc tgatctacga tgcgagcgat ctgcggcgcc
    11461 atctgccgcg cgccgacttt gaaggactgg cgctgaactg aggcattgcg cgggggctgc
    11521 gcgaaaaact tacgctattg caacaaattg ttcgagatgt atcctcgcga aacaagccgc
    11581 cccgcgcggc atgccttccc ctaaatttcc cacttgcaat tttcgccgcc cgtcagcggc
    11641 ctatcccatt tgcaaggaag ctcatgaaga caatgtttcg caatcttgcc ttgtgcgcgc
    11701 cggtggccct gctggccgtt gcctgcggcg gcggttcttc cgataccagc cacggcacgc
    11761 tcggtgtcta tctgaccgac gccccggcat gcggcttcaa cgaggtgaac gtcacgatca
    11821 gcaaggttcg cgtgcatgcc gccgagaacg cgggagagaa cgcctcgggc tggcatgaaa
    11881 tcgtgctcga gccggcacgc cagttcgatc ttctcaagct caccaacggc gtgctcgccg
    11941 aactcggcga ggttgagctg ccggcgggcc ggtacacgca gatgcggctg gtgcttgcac
    12001 cgaacaccgg cgcggcaccg ctggccaatt ccgtcgtgcc agtgggcggg tcgccagttg
    12061 cgctggccac gccgagcgcg atccagaccg gcatcaagct cgtcggcggc ttcgatattg
    12121 ccggcggcga caggaccgaa ctcgccctcg atttcgacgc gtgcaagtcc gtcgtcaccc
    12181 gcgggcacgg cggccatctg ctcaagccgg tcatcagcat cgtgccgatg gcctccagcg
    12241 gcgcgatcag tggcgtgctc gatccgtcga tcaccgcggc agacaatccg atggtctcgg
    12301 cccaggtcga cggcgtgatc gtcaagagca ccgtgcccga tacgaccggc gcgttctcgc
    12361 tggcgccgct gccggcgggg aactatacgg tggtcgtcac cgccgacgcc cgcgccagcg
    12421 acgtgatcac aagcgtaccg gtcacggtca gcaaggacac gccgctcagc accgcggccg
    12481 ccccgctgac gctgcagccg tcggcgaccg ccacggtcag cggcaagctc acgccggccg
    12541 atgcgcaagc gctggtgtcg gccacccaga gtttcgcggc cgggccgcgg gtgacgatca
    12601 agtaccgaaa tgccgatgtc gtgaccggcg attactcgat gacgctgcct accgccgcgc
    12661 cgcgcctcgg ccagttcggc gcgctgccgc tcacgctgac cgcgcagcct gccatcgccg
    12721 ggaagtattc ggtggaggcg agcgcggcgg ggtatcagcc gcgcacgcaa gcggcggacg
    12781 tttcgaccgg tgacgtctcc accgacttca gcctggctcc ctgacccgaa caacgccgcg
    12841 caccgcgcgg cgttgttcat tgccgggtcg tggcaagcgc ttgatccgcc ccgtgcccgc
    12901 ctgcccgggc tgtgtgacca tatggcccgc cgaggtgagc gacatagcaa aggtcttcgc
    12961 tcgaatgtgt tcgcttcaga ctatccgcgc aactgaacaa catccggccc acggcctccc
    13021 aacccgcaaa cagatcaaca acaagaggaa accgccatgc ccctacttgc acccagccga
    13081 acctccgacg atctcggcaa gcttctgctg cgtgtcacgc tcgccttcct gatcctcctg
    13141 cacggcatcg ccaaggtgct gaacggcgtc gatccgatca tgggcatgct ggcgcgtcag
    13201 ggatttccgc cggcactcgc ctatctcgtg tacctgggcg aggtgatcgc gcctgtcctg
    13261 atcatcatcg gcatctggac acgcccggct gcgctgatca tcgcgatcaa catggtggtg
    13321 gcggtctggc tcgtacacat gcgtgaac
//
LOCUS       DATLGF010000117         2502 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_224496, whole genome shotgun sequence.
ACCESSION   DATLGF010000117 DATLGF010000000
VERSION     DATLGF010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224496"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VM406_03465"
     CDS             <1..207
                     /locus_tag="VM406_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008110868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75049.1"
                     /translation="IHKAEIREIDGILYCNDGDWVESLTALVEERSGELRLVTWQEVM
                     AQTARAEHAPAFIDTEEAQCEPAM"
     gene            189..1397
                     /locus_tag="VM406_03470"
     CDS             189..1397
                     /locus_tag="VM406_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVL75050.1"
                     /translation="MRTGYVAEADRLPLETLLGTDFAPTAVPRHAPATLLVISVSAGA
                     GHVRAAQAICAAANTAGRGTARHIDAMDHVLLGLRAIYTDVYLRIVARHPLMWSWLYH
                     KSNAAHADSLWQRLRRASERWSAQRLLSEIEQAQPQAIVCTHFLPAELLARMRREGRL
                     ACPVWVQVTDFDLHRMWVHEGVDGYFAASEEIAYRMRAAGMAADAIRVTGIPVMPAFA
                     QPLERAACAAELGIDARRTTVLLMGGGAGLGRLDETAERLLQAAPEIQMIALAGRNAD
                     VLAALRRLAARHPKRLVALGYTEHVERVMACSDLAITKPGGLSTSECLAMGLPMILTQ
                     PIPGQEERNADYLLEQGAALKTCDMAALEFRLRQLLGHPAQLAEMRKRALSLARVHAA
                     REVVDTVLPS"
     gene            1400..1885
                     /locus_tag="VM406_03475"
     CDS             1400..1885
                     /locus_tag="VM406_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75051.1"
                     /translation="MTSRNAETWRLAATLAYTMMLALLFAQVEIHIEGDAGWAANLPT
                     WRIEDHWMLDVFWGGRPLTGYHATMFSFIGLAFHLPAFFKPRWGWPDEARVLGCLMVF
                     WIVEDFLWFVTNPGYGIGRFTEEHIAWHKHWFLFAPVDYWVFGMIALALLVYSMRSRA
                     A"
     gene            2111..>2502
                     /locus_tag="VM406_03480"
     CDS             2111..>2502
                     /locus_tag="VM406_03480"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HVL75052.1"
                     /translation="MNENAFQPWWVLIFRGVALIAFGAVALILPGITLVVLMALFATY
                     ALIGGAAAVFGSVANRREKLDWGAPLMLGLFSIGTGAIAFLHPGLTALILVLLIAAHA
                     LIVGALDIITAARMHGDQQHEDQGGQAG"
BASE COUNT          400 a          833 c          821 g          448 t
ORIGIN      
        1 atccacaagg ccgagatccg cgaaatcgac ggcatcctgt actgcaatga cggcgactgg
       61 gtcgagagcc tgaccgcgct ggtcgaggag cgcagcggcg aactgcggct ggtgacctgg
      121 caggaagtga tggcgcagac cgcgcgggct gagcatgcgc cggccttcat cgacaccgag
      181 gaagcgcaat gcgaaccggc tatgtagcgg aagcggaccg cctgccgctg gagacgctgc
      241 tcgggacaga ctttgctccc accgccgtcc cgcgccacgc gcctgccacg ctgctcgtga
      301 tctcggtttc cgccggtgcc ggccacgtgc gcgcggcaca ggcgatctgc gccgcagcca
      361 ataccgctgg gcgcggcacg gcacgtcata tcgacgcgat ggaccatgtg ttactcggac
      421 tgcgcgcgat ctacaccgat gtctatctgc gcatcgtcgc gcgccatccg ttgatgtggt
      481 catggctgta ccacaagagc aacgccgcgc atgccgactc gctctggcag cgcttgcgtc
      541 gcgcctcgga gcgctggagc gcgcagcgcc tgctttcgga gatcgaacaa gcccagcctc
      601 aggcgattgt ctgcacccat ttcctgccgg cagagttgct cgcgcgaatg cgccgcgaag
      661 gcaggctcgc ctgtccggtc tgggtgcagg tcaccgattt cgacctgcat cggatgtggg
      721 tgcatgaggg cgtggacggc tacttcgcgg cgagcgaaga gatcgcgtac cgaatgcggg
      781 cggccggcat ggcggccgat gcgatccgcg tgaccggtat tcccgtgatg ccggcgtttg
      841 ctcagccgct ggaacgtgcg gcctgcgcgg ccgaacttgg catcgatgca cgccgcacga
      901 cggtgctgct gatgggcggc ggcgccggtc tgggccggct tgacgagacc gcagaacgcc
      961 tgctgcaggc cgcgccggag atccagatga tcgcgctggc gggacgcaat gccgacgtgc
     1021 tcgccgcgct gcgtcgtctc gccgcgcgcc atccgaaacg cctcgtcgcg ctcggctaca
     1081 ccgagcatgt cgagcgtgtg atggcatgca gcgacctggc gatcaccaag ccgggcggac
     1141 tgtccacctc cgagtgcctg gcgatgggac tgccgatgat cctcacgcag cctatcccgg
     1201 gccaggaaga gcgcaacgcg gattacctgc tcgaacaggg cgcggcgctc aagacctgcg
     1261 acatggccgc gctggagttc cgcctgcgcc agttgctcgg gcatcccgcg cagctggcgg
     1321 agatgcgcaa gcgcgcactg tccctcgccc gcgtgcatgc cgcgcgcgag gtcgtcgata
     1381 ccgtcctgcc ttcctgacca tgaccagccg caatgcagaa acctggcgcc tcgctgccac
     1441 gctcgcctac acgatgatgc tcgcgctgct gttcgcgcag gtcgagatcc acattgaagg
     1501 cgatgccggc tgggccgcca acctgccgac ctggcgcatc gaggatcact ggatgctcga
     1561 cgtgttctgg ggcggccgtc cgctgaccgg ctatcacgcc accatgttca gcttcatcgg
     1621 actggcattc cacctgccgg ccttcttcaa gccgcgctgg ggctggcccg acgaagcgcg
     1681 cgtgctcggc tgcctgatgg tgttctggat cgtcgaggac ttcctctggt tcgtgaccaa
     1741 cccgggttac ggcatcgggc ggttcacgga agaacacatc gcatggcaca agcactggtt
     1801 cctgttcgcg ccggttgatt actgggtgtt cgggatgatt gcgctggcgc tgctggtgta
     1861 ctcgatgcgc tcgcgggcgg catagggttg atgactgtca tcggggtggg gaaggatgag
     1921 attcctcgct gcgctctgaa tgacagctca tttcctgtcg tcccgaggcg cgctgcgacg
     1981 cgggacctca tcgcttcgaa ccgcgatggc atgtgctgcc cgcgaactcc cgccctcccc
     2041 gccgttccaa cccgaaaacg gcgccacccg tcgtactcat cagcaagtga gcaaccacaa
     2101 ggaggacaac atgaacgaaa acgcctttca gccatggtgg gtgctgatct tccgcggcgt
     2161 tgccctgatc gccttcggag cggtcgcgct gattcttccg ggcattacgc tggtcgtgct
     2221 gatggccttg ttcgccacct acgcgctgat cggcggcgcg gccgccgtgt tcggctcggt
     2281 tgccaaccgc cgcgaaaaac tcgactgggg cgcgccgctg atgctcggcc tgttcagcat
     2341 cggcacaggc gcgatcgcgt tcctgcatcc cggcttgacc gccttgatcc tcgtgctgct
     2401 gatcgccgcg catgcgctga tcgtcggcgc gcttgacatc atcaccgccg cacgcatgca
     2461 cggcgatcag cagcacgagg atcaaggcgg tcaagccggg at
//
LOCUS       DATLGF010000118         2357 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_113784, whole genome shotgun sequence.
ACCESSION   DATLGF010000118 DATLGF010000000
VERSION     DATLGF010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113784"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="VM406_03485"
     CDS             complement(<1..1147)
                     /locus_tag="VM406_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004555160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HVL75053.1"
                     /translation="MPAIESKIATSGDAFAQNRAALLAQIEALRALEARTCNASSASK
                     PLFDKRGQMLPRARVARLLDAGAPFLELSTLAGYLMDHKDPEKSIPGGGVIAGIGYVE
                     GVRAMVVASDAGINAGAMKMAGLDKLLRAQHIALAQKLPFIHLVESAGANLLEYRVEH
                     FIHGGSAFCNLARLSAAGLPVITVVHGSSTAGGAYMPGLSDYVVMVRDRARAFLAGPP
                     LLKAATGEIATEEELGGADMHAHVSGLAEYLAENDADALRIARDIVASLDWNRDQPPL
                     RHGWKAPLYDADELLGIMPSDPKKPVDMREVIARIVDGSDFLEFKSAYGPATVVGHAA
                     ICGVRVGILTNNGPLDPAGANKATHFMQACCQSGTPLLFLQNTTGYIV"
     gene            complement(1150..2034)
                     /locus_tag="VM406_03490"
     CDS             complement(1150..2034)
                     /locus_tag="VM406_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012760941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL75054.1"
                     /translation="MSYRSIFRPGLFAGQVAVVTGAGSGIGRCTAHELAALGAKVALV
                     GRNPDKLASVYGELADAGGQASMHICDIRDEDAVLATVEAVLNQHGRIDALVNNAGGQ
                     FPSPLKDISLKGWEAVVRTNLTGGFLMARACLNAWMQAQGGAIVNITADNWGSMPAMG
                     HSGAARAGMVSFTETAALEWAQYGVRVNAVAPGYVASSGMDSYPPQMGPVLRSAMTGV
                     PLQRMASEAEVSAAIVFLLSEAASFITGTVLRVDGGRPQMRAGWTAGIAKSDKPAVRA
                     FDGFPLARPPKIFNPDQD"
BASE COUNT          393 a          798 c          780 g          386 t
ORIGIN      
        1 ccacgatgta gccggtggtg ttctgaagga acagcagcgg cgtgccggac tggcagcagg
       61 cctgcatgaa gtgggtcgcc ttgttcgcgc cggcagggtc cagcgggccg ttgttcgtca
      121 ggatgccgac gcgcacgccg cagatcgccg catgcccgac cacggtggcc ggcccatagg
      181 cggacttgaa ttcgaggaag tccgagccgt cgacgatgcg tgcgatcacc tcgcgcatgt
      241 cgaccggttt cttcgggtcc gagggcatga tgcccagcag ttcgtcggcg tcatacagcg
      301 gcgccttcca gccgtgccga aggggcggct ggtcgcggtt ccagtccagc gaggcgacga
      361 tgtcgcgcgc gatccgcaag gcgtccgcat cgttctcggc aaggtattcg gcaaggcctg
      421 atacatgcgc atgcatgtcg gcgccgccga gttcctcctc ggtggcgatc tcgccggttg
      481 cagccttgag cagcggagga ccggccagga aggcgcgcgc acggtcgcgc accatcacca
      541 cgtaatcgga caggccgggc atataggcac cgcccgccgt cgaggaaccg tgcacgaccg
      601 tgatcacggg caggccggcg gccgacagcc gcgcgagatt gcagaacgcg ctgccgccat
      661 ggatgaaatg ctcgacccgg tactccagca ggttcgcgcc ggcgctctcg accaggtgga
      721 tgaacggcag cttctgcgcg agcgcgatgt gctgggcgcg cagcagcttg tcgagcccgg
      781 ccatcttcat cgcgcccgca ttgatgccgg cgtcggatgc cacgaccatc gcgcgcacgc
      841 cttcgacata gccgatgccg gcgatcacgc cgccgcccgg aatgctcttt tccgggtcct
      901 tgtggtccat caggtaaccg gcgagcgtcg acagttcaag gaacggcgcc ccggcatcga
      961 gcaggcgcgc gacccgggcg cgcggcagca tctgcccgcg cttgtcgaac agcggcttcg
     1021 atgcggagga ggcattgcag gtgcgggctt cgagcgcacg caaggcttcg atctgcgcca
     1081 gcagcgccgc gcggttctgc gcaaacgcgt cgccggatgt cgcaatcttc gattcgatgg
     1141 ccggcatgct cagtcctggt ccgggttgaa gatcttcggg gggcgcgcaa gcggaaagcc
     1201 gtcaaacgcg cgtacggccg gtttgtcgga cttcgcgatg ccggcagtcc agccagcgcg
     1261 catctgcggc cggccgccgt cgacgcgcag cacggtgccg gtgatgaagg aagcggcctc
     1321 ggacagcaga aacacgatcg ccgccgacac ttccgcttcg ctggccatgc gctgcagcgg
     1381 cacgcccgtc atcgcgctgc gcagcaccgg ccccatctgc ggcggatagg aatccatgcc
     1441 gctggaggca acatagccgg gcgcaaccgc attcacgcgc accccgtact gcgcccattc
     1501 aagcgcggcg gtctcggtga aactgaccat gccggcgcgc gcggcgccgg aatgccccat
     1561 cgccggcatg ctgccccagt tgtcggcggt gatgttgacg atcgcgccgc cctgcgcctg
     1621 catccatgca ttcaggcagg cgcgcgccat caggaatccg ccggtcaggt tcgtgcgcac
     1681 gaccgcctcc cagcctttca gtgaaatgtc cttcagcggc gacggaaact gcccgcccgc
     1741 attgttgacg agcgcatcga tacggccatg ctggttcagc accgcctcga cggtcgccag
     1801 gaccgcatcc tcgtcgcgga tgtcgcagat gtgcatgctg gcctggccgc cggcgtcggc
     1861 gagctcgccg tagaccgacg ccagcttgtc cggattgcgc ccgaccagcg cgactttcgc
     1921 gccgagcgcg gcgagttcgt gcgcggtaca gcggccgatg cccgagccag cgccggtgac
     1981 gacggcaacc tgcccggcga acaggccggg gcggaatatg gagcggtagg acatggtctc
     2041 ctcgttgttc tgttctgttc tgttcgtcgg gcttccgaac cgtgacggca ctgcgcatgg
     2101 tcagggagat ccgatctaac gcgatgcccg tgatctgtcg ctcccgcgca ggcgggagcc
     2161 cagttcagac tccagcagta ctgcgaaccc cgagccaggc tccgcctgcg gaacgacggg
     2221 caagccgacg ccctcaaggc tgcgacccat tctactaacc aagcagttgc tttgtaaagc
     2281 cgcgaccctt atgattgctc cacgctttca caccgatctg tgaggagacc cccgtgaccg
     2341 acgcatatgt atacgac
//
LOCUS       DATLGF010000119        15660 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_21583,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000119 DATLGF010000000
VERSION     DATLGF010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 15660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15660
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21583"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VM406_03495"
     CDS             complement(<1..275)
                     /locus_tag="VM406_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75055.1"
                     /translation="MTSLPLSLRSRTTRTLLVGFLATLAILALVCTYGFLRVHKMNED
                     LRDAAQGRAHKIALIEGMQSLTRDAVSALYLSQMQPGAADAASRKTI"
     gene            548..1423
                     /gene="asd"
                     /locus_tag="VM406_03500"
     CDS             548..1423
                     /gene="asd"
                     /locus_tag="VM406_03500"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666823.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HVL75056.1"
                     /translation="MSRFFILHQYLLPKQALTELAGRFARGEGGRFTQPVIRWFIQRY
                     GVDMTEAQNPDVASYASFNDFFTRALREDARPLADADFICPVDGAISQFGPLEHDQII
                     QAKGHRYSSTALLGGDASLARMFDHGSFATLYLSPRDYHRIHMPCDGRLTRMIYVPGA
                     LFSVNPATARGVPGLFARNERVVCVFESAFGPFALVLVGATIVGSMATVWHGVVNPPR
                     SRTVREWHYRDQEIVLAKGAEMGRFLLGSTVVMLFPKDVLEFNREWTATRAIRMGEAM
                     GHKPRGSRYDTPGNT"
     gene            1401..1817
                     /locus_tag="VM406_03505"
     CDS             1401..1817
                     /locus_tag="VM406_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HVL75057.1"
                     /translation="MTHQAILEFSTAGRGTRDITAEVARVVEQAGVKAGIVHVFVQHT
                     SCSLTITENADPDVRRDLETLLGRLAPDGDPAYRHDLEGPDDMAAHGRSVLTGPALSV
                     PVGDGRMLLGTWQGIFLWEHRTGPQRRRVVVTVLSG"
     gene            1885..3012
                     /locus_tag="VM406_03510"
     CDS             1885..3012
                     /locus_tag="VM406_03510"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HVL75058.1"
                     /translation="MPFTDLKTQYRALESALHGRIQRVLEHGQFILGPEVAELETALA
                     GYTGARHCVTVANGTDALQIALLALGVGPGDEVITPAFSFVASAEAIVLAGAVPVFVD
                     VEADTGNVDATKIGPAIGPRTRAILPVSLYGQPPDMQAISALAQAHGLAVVEDGAQSF
                     GACYRDMRSGNLSTIGCTSFYPSKPLGCYGDGGALFTNDDALAEAARQLRDHGQSGRY
                     LHTRIGLNSRMDSLQCAILLAKLERFPWELERRAALGRRYGELLDAAGVARIRQRRDR
                     SSVFAQYTILADDRARVCALLQEAGIPSAIHYPLPLNRQPAYAHYPQTDGCPVAAELS
                     ARVLSLPMSADLAEPDQSRIVERVAGAVRDVRNKPPHRSGA"
     gene            complement(2897..3985)
                     /locus_tag="VM406_03515"
     CDS             complement(2897..3985)
                     /locus_tag="VM406_03515"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HVL75059.1"
                     /translation="MKLAIIGCGRQGERHLAGLRESRYAAIPVVVADLHASRSRGLAA
                     RFHTCDAATVDAALADRDVGAALICTPTMSHAALCARALQAGKHVLCEKPLCDSLSQA
                     ARLHALARQADRVLAVGYIYRHAPRLAAARAALVEDSFGSPATPLGKVLTAWLRAGGR
                     GDQSRWKHQRADGGGAVNEMLVHMIDLGIWIFGRCERVHVLAKKTLNPVRSIAGETFS
                     PDTEDFVIVKMACAGGLEYFIEADLATPGFVQYLDVVGENGNLFTSILPEFPSYLFLR
                     TEKDGYPAGRSVLGEGPGNYYVPQIEAFLDATQGRPAPSLHRAEDSLEVMRLIDEVAC
                     SGHPAPPRPRAPRSATGLALPGRRSWEG"
     gene            complement(4177..4833)
                     /locus_tag="VM406_03520"
     CDS             complement(4177..4833)
                     /locus_tag="VM406_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVL75060.1"
                     /translation="MHLPLTPASAPTTAGYRERTWQKKHADLLRAATRLFAQQGIVNT
                     STSEVAAAAKSTERTLFNHFQSKEGLVRAVIAEAIVPHLAERAVSDVRALLGCAPEEL
                     EQHIAGLLSARRLAYEENTELTRLLLMELVRDEAVRKDFGAQWYAQIWLPIRDLFAEL
                     QAGGKLRHELSADTLATIFYSITVGYLIGRTILAPEAPWRDEQDVQDLARFFLRGAGA
                     "
     gene            complement(4888..5595)
                     /locus_tag="VM406_03525"
     CDS             complement(4888..5595)
                     /locus_tag="VM406_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HVL75061.1"
                     /translation="MSDLSAFPITKKWPARHPERIQLYSLPTPNGVKVSIMLEETGLP
                     YEAHLVSFQHNDQTSPEFLSLNPNNKIPAIIDPAGPGGKPLPLFESGAILLYLADKTG
                     KFLPDDPALRYQTVQWLMFQMGGIGPMFGQLGFFHKFAGREYEDKRPRDRYVAEAKRL
                     LGVLEQRLAGHAWIMGDEYTIADIATFPWVRNLVGFYDAGELVGFGDYPQVKRALDAF
                     AARPAVVAGLHVPARPA"
     gene            5759..7267
                     /locus_tag="VM406_03530"
     CDS             5759..7267
                     /locus_tag="VM406_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HVL75062.1"
                     /translation="MIASPDNLLPVFTLASSFIPGLIIFFLDESRENLRVFLNMAGAT
                     IKLVFVGIMLWGVTQGYEYRFSHRVVPGLELVLQVDALALLFVTLSAVLWFLTTLYAI
                     GYLQHSADRNRFFGFFSLCVTTTVGIALAGNLFTFFFFYELLTLATYPLVVHRGNAAS
                     LHAGNRYLAYTLGGGALLLVGTVWLYALCGTVDFTHTGNAARAGPEAATQLRIIFLFL
                     IAGLGVKASLIPLHGWLPSAMVAPAPVSALLHAVAVVKAGAFGVVRVLYEVYGIEVAH
                     GLGLLLPLALLASATILWGSLRALFQDDLKRRLAYSTVSQVSYIVLGVALFGPLGTIG
                     GLAHLIHQGIMKVTMFFCAGNYAETLGIHKVTELDGVGARMPWNTLAFTCAALGMIGV
                     PPMAGFISKWYLGLGALDSGAGWALALLAVSSLLNAAYFLPILYRAWFKHPAAPFENL
                     IPRRGRWETSRLLLIPPLATAVMTVAAGLFAAYPISPLGWAQLIVSLQYHGL"
     gene            7270..8922
                     /locus_tag="VM406_03535"
     CDS             7270..8922
                     /locus_tag="VM406_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 5 family protein"
                     /protein_id="HVL75063.1"
                     /translation="MHALILLAFLLPLGTAALLLLRAVRPAARAAAPFTPLPALALAV
                     LAPAGTTVEVPWLLLGARFGVDETGRMFLLFTSFVWLIAGWFARGYLHGDQKRTRFWA
                     FFLATQTGNLGLCLALDAATFYLLFALMTFCGYGLVIHAGTAEALRAGRVYLAMAMLG
                     EAMLVAGILLLVYSGQNNHYFDALAAAPVTQLHATLLLCGFGIKAGILLLHMWLPLAH
                     PVAPIPASAVLSGVMLKAGVLGWVRYLPLGSLALPETGQVLMGVGLLAMFYGVAVGVT
                     QSNMKVLLAYSSISQMGFIALGVGAGLLAPQLWPLLLPAVTLYAFHHALAKSALFLGC
                     GVVASTRKRHRVLAALAIPALALAGLTFTSGALVKSGLKVALADVPAPWPAWLHWLLP
                     LAAVGTTLLMARFMLLAARRESNEHHRGMVLPWSLLLIAVLGSTWLLAPEGGFAYALK
                     AGTLLSSTWPVVAGLILAAIAARSGRRVPAVPPGDLLLPVEKCLAWLGRCTLTAVSWL
                     PSVLWDDRDLHLQLERIARHFTVERTVRGVLLLFIALTVARF"
     gene            complement(8955..9149)
                     /locus_tag="VM406_03540"
     CDS             complement(8955..9149)
                     /locus_tag="VM406_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75064.1"
                     /translation="MTNQKTPEQKPEDPKRADARTEAEAKSVDGKAGQGNKSSHDHMS
                     HIKANTHEGAGGGAKAKRGH"
     gene            complement(9341..10684)
                     /gene="gdhA"
                     /locus_tag="VM406_03545"
     CDS             complement(9341..10684)
                     /gene="gdhA"
                     /locus_tag="VM406_03545"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HVL75065.1"
                     /translation="MKYQSLEEFLDYVKARNPGQPEFHQAVVEVMESLWPFIAKHPRY
                     AEHGLLDQLVEPERVIQFRVSWVNDKGEVQVNRGYRIQHSSAIGPYKGGLRFHPSVNL
                     SILKFLAFEQTFKNALTTLPMGGGKGGSDFDPKGKSPGEVMRFCQAFAAELFRHVGSD
                     TDVPAGDIGVGGREVGFMVGMVKKLTNRSDCVFTGKGLSFGGSLIRPEATGYGTVYFV
                     EEMLRQKGRSLEGMRVSVSGSGNVAQYAVEKAMACGAKVVTVSDSSGTVVDEEGFTPE
                     KLAVLMEVKNHYYGRVNDYAEKVGAKFIAGATPWNVKVDVALPCATQNELDEKDAATL
                     IANGVICVAEGANMPCTAEAAKLFEEKGVLYAPGKASNAGGVATSGLEMTQNAMRLSW
                     NRDEVDERLKSIMVNIHAACMKYGQREDGSVSYIDGANIAGFVKVADAMLAQGVI"
     gene            10980..11519
                     /gene="gloA"
                     /locus_tag="VM406_03550"
     CDS             10980..11519
                     /gene="gloA"
                     /locus_tag="VM406_03550"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HVL75066.1"
                     /translation="MPFTTENHPGVHQDTPAATRGFTLNHTMVRVKDPAISLDFYTRI
                     LGMRVLRKLDFPEMKFSLYFLARLDENEAPPEDVGERTAWTFSQRGILELTHNWGTES
                     DAEFRYHDGNAQPQGYGHICFSVPDLAAAIRWFDENNVQYVKRPEQGKMRDVAFIKDP
                     DGYWIEIVQPGLLKALGRS"
     gene            complement(11570..12244)
                     /locus_tag="VM406_03555"
     CDS             complement(11570..12244)
                     /locus_tag="VM406_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312699.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HVL75067.1"
                     /translation="MPAPHAPGRPASRSRAARPLPPRVAPQTDSLDDVRAAARGCQAC
                     PLWAGATQTVFGEGAEHGRVMLVGEQPGDQEDLSGHPFVGPAGRLLDRALAEAGIERD
                     EVYVTNAVKHFKYELRGKRRLHKKPGDVEIAACVQWLERELDLVAPALVVALGATAAR
                     ALLGRATPIEANRGRLMSFGEDRQLLITTHPSALLRMPGELKAAAYARFVADLRIAAP
                     FASGSG"
     gene            12503..14350
                     /locus_tag="VM406_03560"
     CDS             12503..14350
                     /locus_tag="VM406_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HVL75068.1"
                     /translation="MVRNAHARSASFGLHTAENPEYSPLTRSALGAVIEQNHALHQYA
                     LPVMETLYEQIVNTHSMVILTDAQGVIMHALGDDDFLEKARRVALAPGVAWSEQRRGT
                     NAIGTAIAEAAPALVHADEHYLRANHVLTCSAAPIFDAAGTLAGVLDVTGDRHSFHPH
                     TMALVRMSAQMIENQLLAAACADGITLRFHSRAEFLGTLMEGIVTFAPGGRCISANRS
                     AQFQLGLAPTALRTHTLQSLFGIGVSAMVDHYRTAMPDPLTLFLPSGVRVQARAEVRL
                     SHAASIAASGARASAGAPRQTATVRPRSALHALDTGDPQIAALIARLEKIKGRDIPVV
                     IHGETGTGKELLAHAIHRDSPRAAGPFIAVNCAAIPESLIESELFGYEDGAFTGARRK
                     GHQGRILQADGGTLFLDEIGDMPPALQARLLRVLQERMVTPLGGSRCVPVNVAIICAT
                     HRNLRDMMTAGSFREDLYYRLNGLVVKLPPLRERQDFDAVIGKLLAAEAPERRYRIAP
                     EVMAQFRRHPWPGNLRQLANLVRTAVVMAGDDEVIGLAHMPDDFLDDLAPSDVPAARC
                     TRAPAAGSGRLEDVEAQAIRAAIGAHGGNVSAAARALGISRNTIYRKLR"
     gene            complement(14343..14723)
                     /locus_tag="VM406_03565"
     CDS             complement(14343..14723)
                     /locus_tag="VM406_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008347693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HVL75069.1"
                     /translation="MNDETVPPRVLATEAARELIAALKARHGKLMFFQSGGCCDGSAP
                     MCFPEGEFPVGAADVRLGDLDGVPFYIGGDQYEYWKHTQLIIDVVPGGGGMFSLENGS
                     GKRFLTRSRLFTDAEYARLMQDAT"
     gene            complement(14900..>15660)
                     /gene="adh"
                     /locus_tag="VM406_03570"
     CDS             complement(14900..>15660)
                     /gene="adh"
                     /locus_tag="VM406_03570"
                     /EC_number="1.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HVL75070.1"
                     /translation="NIIPVTLELGGKSPNVFFEDVMAADDDYFDKCLEGFAMFALNQG
                     EVCTCPSRVLVQESIFDRFMERALARVAAIRQGNPLDSQTMIGAQASQEQLEKILSYL
                     DIGRQEGAQVLAGGERNVLDGDLEKGYYVKPTVFKGHNKMRIFQEEIFGPVVSVTTFK
                     DEDEALEIANDTLYGLGAGVWTRDGARAFRMGRGIEAGRVWTNCYHLYPAHAAFGGYK
                     QSGIGRENHKMMLAHYQQTKNLLVSYSPKALGFF"
BASE COUNT         2448 a         5331 c         5055 g         2826 t
ORIGIN      
        1 tcgatcgtct tgcgactggc cgcgtctgcc gcaccgggct gcatctggct cagatagagc
       61 gccgagaccg cgtcgcgcgt gaggctctgc atgccttcga tcagcgcaat cttgtgcgcc
      121 ctgccctgcg cggcgtcgcg caggtcttca ttcatcttgt gcacgcgcag aaagccgtag
      181 gtgcagacca gggccaggat ggccagcgtg gcgaggaagc cgacgagcag ggtgcgcgtc
      241 gtgcggctgc gcaaggacag cggcagggac gtcatgcccg gcgcagcttg taacgggtat
      301 gcgttccctc cagcgggtct tcgcttcggt tgcaagcggc gcagtgcggc ctcgggtccc
      361 tggttttcac gcgtggtgtc ggtcgattaa cgtgcggcaa acgatagtgg tgttcccggc
      421 aggcgtcaag ccggttcctg ccgcagcgtc cgccttcgcc ccttgcacgg ggacccggac
      481 cgccctttcg gcaggacgcc gcatgtcacg cggctataat cggggcgatt caatttcggg
      541 atttgccgtg tcgcgtttct tcatactcca tcaatacctt ctacccaagc aggcactgac
      601 cgaactcgcg ggccgcttcg cgcgcggcga gggcggccgc ttcacgcagc cggtgatacg
      661 ctggttcatc cagcgctacg gcgtcgacat gaccgaggcg cagaaccccg acgtcgccag
      721 ctacgcaagc ttcaacgatt tcttcacgcg cgcgctgcgc gaagatgcac gcccgctcgc
      781 cgacgccgac ttcatctgcc cggtagacgg ggcgatcagc cagttcggcc ccctcgaaca
      841 tgaccagatc atccaggcca agggacaccg ctattcgagc accgcactgc ttggcggcga
      901 cgcgtcgctg gcgcggatgt tcgaccacgg cagctttgcc acgctgtacc tgagcccgcg
      961 cgattaccat cgcatccaca tgccgtgcga cggccgcctg acgcgcatga tctatgttcc
     1021 cggcgcgctg ttctcggtca atccggccac cgcgcgcggg gtgcccggcc tgttcgcgcg
     1081 caacgaacgc gtcgtctgcg tcttcgaatc cgcgttcggc ccgtttgcgc tggtgctggt
     1141 cggcgccacc atcgtcggca gcatggcgac ggtctggcat ggcgtggtga acccgccgcg
     1201 ctcgcgcacc gtgcgcgaat ggcactaccg cgaccaggag atcgtgctgg caaaaggtgc
     1261 ggaaatgggt cgcttcctgc tcggctccac ggtcgtgatg ctgttcccga aggacgtgct
     1321 cgagttcaac cgggaatgga ctgccacgcg cgcaatccgc atgggcgagg caatggggca
     1381 caagccgcgg gggagccggt atgacacacc aggcaatact tgaattttcc accgccggcc
     1441 gcggcacgcg cgacatcacg gccgaggtcg cgcgggtggt cgagcaggct ggcgtgaagg
     1501 ccggcatcgt ccatgtgttc gttcagcaca ccagttgctc gctgacgatc accgagaacg
     1561 ccgatcccga tgtgcggcgc gatctggaaa ctttgctcgg gcggcttgcg cccgacggcg
     1621 atcccgccta ccgccacgat ctcgaaggtc cggacgacat ggccgcgcac gggcgcagtg
     1681 tgctgaccgg ccctgccctt tccgtgcccg tcggcgacgg ccggatgttg cttggtacct
     1741 ggcaaggcat cttcctgtgg gaacaccgta ccggcccgca gcggcggcgc gtcgtcgtca
     1801 cggtactcag cggctgaagc gtcaaccatc ggcgctgcgc gctgtcacac cggcagcagg
     1861 aaccttccac gccggccgcc gatgattccg tttaccgatc tgaagacgca gtaccgtgcg
     1921 ctcgagtctg cattgcatgg gcgcatccag cgcgtgctcg agcacggcca gttcatcctc
     1981 ggccccgagg ttgccgaact cgagaccgcg ctcgccggct acacgggcgc gcgccactgc
     2041 gtcaccgtcg ccaacggaac cgatgcgctg cagatcgcgc tgctggccct tggcgtcggg
     2101 cccggcgacg aagtgatcac gcccgcgttc agcttcgtcg cctccgccga ggcgatcgtg
     2161 ctggccggcg cggtgccggt gttcgtcgac gtggaagccg ataccggcaa tgtcgatgcc
     2221 acgaagatcg ggcccgccat cgggccgcgc acccgggcga tcctgcctgt ttcgctgtac
     2281 ggccagccgc cggacatgca ggccatcagc gcgctggcgc aagcgcacgg cctggccgtc
     2341 gtcgaggacg gcgcgcaaag cttcggcgcc tgctaccgcg acatgcgtag cggcaatctc
     2401 tccacgatag gctgcaccag cttctatccg agcaagccgc tgggctgcta cggtgacggg
     2461 ggagcgctgt ttacgaacga tgacgcgctc gccgaggccg cgcggcagct gcgcgatcac
     2521 ggccagtcag ggcgctatct gcacacgcgt atcgggctca acagcaggat ggacagcctg
     2581 cagtgcgcga tcctgctcgc caagcttgaa cgcttcccat gggagctcga acggcgcgca
     2641 gcgctcggcc ggcgctatgg cgaactgctc gacgcggccg gcgttgcccg catccggcaa
     2701 cgacgcgacc gcagcagcgt gttcgcgcag tacacgattc ttgcggacga tcgcgcgcgc
     2761 gtgtgcgcgc tgctgcagga agccggcatt ccgagcgcga tccactatcc attacccctc
     2821 aatcgccagc cggcctatgc gcactatccg cagaccgacg gctgcccggt cgcagcggaa
     2881 ttgtctgcac gcgtgctcag ccttcccatg agcgccgacc tggcagagcc agaccagtcg
     2941 cggatcgtgg agcgcgtggc cggggcggtg cgggatgtcc ggaacaagcc acctcatcga
     3001 tcaggcgcat gacctccaga ctgtcttccg cccggtgcag cgacggcgcc ggacggccct
     3061 gcgtggcgtc gaggaaggct tcgatctggg gtacgtaata gttgccaggt ccttcgccca
     3121 gcacgctgcg tccggccgga tagccgtcct tttccgttct caggaacaga tagctgggga
     3181 attccggcag gatcgacgtg aacaggttgc cgttctctcc gaccacgtcg aggtactgca
     3241 cgaaaccggg cgtggccagg tccgcctcga tgaagtattc gaggccgccg gcgcaggcca
     3301 tcttgacgat gacgaagtcc tccgtatccg gcgagaaggt ttcgcctgcg atcgagcgca
     3361 ccgggttaag cgttttcttg gccagcacat gcacgcgttc gcatcggccg aaaatccaga
     3421 tgcccaggtc gatcatgtgc accagcatct cgttgaccgc tccgccgccg tcggcgcgct
     3481 ggtgtttcca gcgcgattgg tcgccgcgcc cgcccgcgcg cagccatgcg gtcaggacct
     3541 tgcccagcgg agtggcggga ctgccgaaac tgtcttcgac gagtgctgcc cgcgccgccg
     3601 caagccgcgg cgcgtgacga tagatgtagc cgactgcgag tacccggtcg gcctgccgcg
     3661 ccagcgcatg cagccgcgcg gcctgcgaca gactgtcgca caagggtttc tcgcacagca
     3721 cgtgcttgcc ggcctgcagc gcgcgtgcgc acagcgccgc gtgcgacatg gtcggcgtgc
     3781 agatcagcgc ggcgccgaca tcccggtcgg ccagtgccgc atccaccgtc gctgcgtcgc
     3841 atgtgtggaa tcgtgccgcc agtccacgcg aacgcgacgc atggagatcg gcaacgacaa
     3901 ccggaatggc tgcgtatctg ctttcgcgca ggccggccag gtgccgctca ccctggcggc
     3961 cgcagccgat gatcgcgagt ttcatgatgt tggaggcgct ctcgttcgcg cagcgggact
     4021 gcggggaaga gcgcgccttg cgcccgcttc tcgcccccgg catgcgccgt cgaagttgga
     4081 gtcgcgcatg cgccgctgtg atcggaagaa tgtttgagcc cgatcaggat ttcgaccggg
     4141 agccggccaa tggcgcggcc ccggtcttgc gccgcgtcac gccccggcgc cgcgcaggaa
     4201 gaagcgggcg agatcctgca cgtcctgctc gtcgcgccat ggcgcttccg gcgcgagtat
     4261 ggtgcggccg atcaggtagc cgaccgtgat cgagtagaag atcgtcgcca gcgtgtcggc
     4321 ggagagctcg tgcctcaatt tgccgcctgc ctgcagctcg gcgaacagat cgcgtatcgg
     4381 cagccagatt tgcgcatacc attgcgcacc gaaatccttg cgcaccgctt cgtcgcgcac
     4441 gagctccatc agcagaagcc gggtcagctc ggtgttttcc tcatacgcga ggcgccgggc
     4501 gctgagcagg cccgcgatgt gctgttcgag ttcttcaggc gcgcagccaa gcagcgcgcg
     4561 cacgtcggac accgcccgtt cggccaggtg cggcacgatg gcctcggcga tcaccgcgcg
     4621 aaccaggcct tccttcgact ggaaatgatt gaacagcgtg cgttcggtcg atttggccgc
     4681 tgccgccacc tcgctggtgc tggtgttgac gatgccttgc tgggcgaaca gccgggtcgc
     4741 ggcgcgcagc agatccgcgt gtttcttctg ccacgttcgt tcccggtaac cggcggtggt
     4801 gggtgcggaa gcgggagtga gagggagatg catgggaaag aggaaattgc tgggcggcaa
     4861 gtatagtgct tgcgtctcaa tcctgcttca ggccggtcgc gccggaacgt gcagccccgc
     4921 cacgaccgcc ggccgcgccg caaacgcatc gagcgcccgc ttcacctggg gatagtcgcc
     4981 aaagccgacg agctctccgg cgtcgtagaa gccgaccaga ttgcgcaccc aagggaaggt
     5041 cgcgatatcg gcgatcgtgt actcgtcacc catgatccac gcatgcccgg caagtctttg
     5101 ctcgagcacg ccgagcaggc gtttcgcttc ggcgacatag cggtcgcgcg gacgcttgtc
     5161 ctcgtattca cgtccggcga atttatggaa gaagccgagc tggccgaaca tcggcccgat
     5221 gccgcccatc tggaacatca gccactgaac ggtctggtag cgcagcgcgg gatcgtcggg
     5281 caggaacttg ccggtcttgt ccgcgagata gagcaggatc gcgccggatt cgaacagcgg
     5341 cagcggcttg ccgcccggtc ctgcgggatc gatgatggcc gggatcttgt tgttcgggtt
     5401 caaggacagg aactcgggcg aggtctggtc gttgtgctga aaactgacca gatgcgcctc
     5461 gtacggcaac ccggtctcct ccagcatgat cgacaccttc accccattgg gcgtaggcag
     5521 cgagtacagc tggatgcgct caggatggcg cgccggccat ttcttcgtga tcgggaaggc
     5581 ggagagatcg ctcatgtcgg ttcgtgtcgg aatcatgggg aagcgaacat cttagagcgg
     5641 attgcagatc ggcgccgggc gcgcctgcgg cgcccaaaac tcgcaccggc caaacgcatc
     5701 caaccgaaag atgctccgta ataacacgcg gccgttccag cgtcgtcgcc cctcaacaat
     5761 gattgcatcg cccgacaatc tgcttccggt gttcacgctg gcgagttcgt tcattcccgg
     5821 cctgatcatc tttttcctcg acgagtcgcg cgagaatctg cgcgtattcc tcaacatggc
     5881 cggcgccacg atcaagctgg tcttcgtcgg catcatgctc tggggcgtca cccagggata
     5941 cgagtaccgt ttcagtcacc gcgtcgtgcc agggctcgag cttgtgctgc aggtcgatgc
     6001 gcttgcgctg ctgttcgtca cgctctctgc agtgctgtgg tttctcacca cgctctatgc
     6061 gatcggctac ctgcagcatt cggccgaccg caaccgcttc ttcggcttct tcagcctttg
     6121 cgtgacgacc accgtcggga tcgcactggc cggcaacctg ttcaccttct tctttttcta
     6181 cgagctgctc acgctggcca cctacccgct cgtggtccat cgcggcaacg ccgcctcgct
     6241 gcacgctggc aaccgctatc tcgcctacac gctgggcggc ggcgcccttc tcctcgtggg
     6301 taccgtgtgg ctgtacgcac tgtgcggcac agtggacttc acccacaccg gcaacgcggc
     6361 ccgcgccggc ccggaggcag cgacgcagct gcgcatcatc ttcctgttcc tgatcgcggg
     6421 gctgggcgtc aaggcctcgc tgatccccct gcacggctgg ctgccctctg caatggtggc
     6481 accggcgccg gtctcggcac tgctgcatgc ggttgccgtg gtcaaggccg gcgccttcgg
     6541 cgtggtgcgc gtgctgtacg aggtctacgg catcgaagtc gcccacgggc tcgggctgct
     6601 gctcccgctg gcgctgctgg cttcggcgac gatcctgtgg ggctcgctgc gcgcgctgtt
     6661 ccaggatgac ctcaagcgcc gcctggccta ctctacagtg agccaggtct cctacatcgt
     6721 gctgggcgtc gcgctgttcg gaccgctggg cacgataggc gggctcgccc atctgatcca
     6781 ccagggcatc atgaaggtca cgatgttctt ctgcgccggc aattacgccg agacgctcgg
     6841 cattcacaag gtcacagagc tcgacggcgt gggtgcgcgc atgccttgga acacgctggc
     6901 gttcacctgc gcggcgctcg gcatgatcgg cgtgcccccg atggccggct tcatcagcaa
     6961 gtggtacctg gggctcggcg cgctcgactc gggcgccggc tgggcgctgg cgctgctggc
     7021 ggtatccagc ctgctcaatg ccgcctactt cctgccgatt ctctaccgcg cctggttcaa
     7081 gcatccggcc gcgccgttcg agaacctgat cccgcgccgc ggccgctggg aaacctcgcg
     7141 gctgctgctt atcccgccgc ttgcgacggc cgtgatgacg gttgcagccg gcctgttcgc
     7201 cgcatatccg atcagcccgc ttggctgggc gcagctgatc gtcagtcttc aataccacgg
     7261 gctgtgacga tgcatgcgct gattctgctt gccttcctgc tgccgctcgg caccgccgcg
     7321 ctgctcctgc tgcgcgccgt gcgtccggcg gcccgggccg cggcgccctt cacgccgctg
     7381 ccggcactgg cgctggccgt gctggcccct gcaggaacca cggtcgaagt cccgtggctc
     7441 ctgctcggcg cacgcttcgg cgtcgacgaa accgggcgca tgttcctgct ttttaccagc
     7501 ttcgtctggc tgatcgcggg ctggtttgcg cgcggctacc tgcacgggga ccagaaacgc
     7561 acgcgcttct gggcgttctt cctcgcgacc cagaccggca atctcgggct ctgccttgcg
     7621 cttgacgccg ccaccttcta tctgctgttc gcgctgatga ccttctgcgg ctatggcctg
     7681 gtgatccacg ccggcactgc cgaagcgctg cgtgccgggc gcgtctatct cgcgatggcg
     7741 atgctgggcg aggcaatgct ggtggccggc atcctgctgc tggtgtactc aggccaaaac
     7801 aaccattact tcgatgcgct cgccgctgcg cccgtcacgc agctgcacgc gacgctgctg
     7861 ctgtgcggct tcggcatcaa ggccggcatt ctgctcctgc acatgtggct gccgctggcc
     7921 catccggtcg cgccaattcc cgccagcgcc gtgctgtccg gcgtcatgct caaggccggc
     7981 gtgctgggct gggtgcgtta tctcccgctt ggctcgctcg cgcttccgga aaccgggcag
     8041 gttctgatgg gcgtggggct gctggcgatg ttctatggcg tcgccgtcgg ggtgacgcag
     8101 tccaacatga aggtgctgct ggcgtactcc agcatcagtc agatgggatt cattgcgctc
     8161 ggcgtcggcg ccggtctgct ggcgccgcag ctctggcccc tgctgctgcc ggcggtgacc
     8221 ttgtacgcgt tccatcatgc gctggcgaaa agcgccttgt tcctcggctg cggcgtcgtc
     8281 gcttcgacgc ggaagcggca ccgcgtgctg gccgcgttgg cgatcccggc gctggccctc
     8341 gcgggcctca cgttcaccag cggcgcgctg gtgaagagcg gcctgaaagt cgcgctggcc
     8401 gatgtgccgg cgccctggcc ggcatggctg cactggctgc tgccgctggc ggccgtcggc
     8461 accacgctgc tgatggcgcg cttcatgctg ctggccgcaa ggcgcgaatc caacgaacac
     8521 caccggggca tggtattgcc ctggagcctg ctgctgattg ccgtgctcgg ctccacctgg
     8581 ttgctggccc ctgaaggcgg gttcgcgtac gcgctcaagg ccgggacact gctgtcgtcg
     8641 acctggccgg tcgttgcggg actgatcctg gccgccatcg cggcccgttc ggggcgccgt
     8701 gtcccggccg tgccgcccgg agacctcctg ctgcccgtcg aaaagtgcct tgcctggctg
     8761 ggacgctgca cgctgacggc ggtcagctgg ctgccgtcgg tgctctggga tgaccgcgac
     8821 ctgcacctgc agcttgagcg catcgcacgc cacttcaccg tggaacgcac ggtgcgcggc
     8881 gtgctgctgc tgttcattgc gctgacggtt gcgcgcttct gaacgcagcg cggcgcagca
     8941 tcagggatcg cgactcagtg cccgcgcttg gccttggcgc cgccgccggc gccttcgtgg
     9001 gtgttggcct tgatatggct catgtggtcg tggctgctct tgttgccttg accggctttg
     9061 ccgtcgaccg atttcgcttc cgcctcggtc ctggcatccg cgcgcttcgg atcttccggt
     9121 ttctgctctg gggtcttctg gttcgtcatc acggtccttt cgcaaactgt ggagctactc
     9181 atgcgattga caggatggac gcgggaaagg tcccggtggg gcaggatgtt gcgggggaaa
     9241 gaaattgaac cacgcgaacg cgacgagcac tgcggcgcgc tctgcgtggt tgctgtcccg
     9301 gcaccgccgg ccgcgggccg gggtgcaggc aatacaagga tcagatcacg ccctgggcga
     9361 gcatcgcgtc ggccactttc acgaagccgg cgatgttggc gccgtcgatg tagctgacgc
     9421 tgccatcttc acgctggccg tacttcatgc aggccgcatg gatgttgacc atgatgctct
     9481 tgaggcgctc gtcgacctcg tcgcgattcc aggacaggcg catcgcgttc tgggtcatct
     9541 ccaggcccga ggtggcaacg ccgcccgcat tgctggcctt gcccggcgcg tacagcacgc
     9601 ccttttcctc gaacagcttg gccgcctcgg cggtgcaggg catgttcgcg ccttcggcca
     9661 cgcagatcac gccgttggcg atcagggtgg cggcgtcctt ctcgtccagt tcgttctgcg
     9721 tcgcacacgg cagtgcaacg tcgaccttca cattccacgg cgtggcgccg gcgatgaact
     9781 tcgcgccgac cttctccgcg tagtcgttga cccggccgta gtagtggttc ttcacttcca
     9841 tcagcaccgc gagtttttcc ggcgtgaagc cttcctcgtc gaccacggtg ccggaagaat
     9901 cggacacggt cacgaccttg gcgccgcagg ccatcgcctt ctcgaccgcg tactgggcca
     9961 cattgcccga accggacacc gagacgcgca tgccttccag cgagcggccc ttctgcctga
    10021 gcatttcctc gacgaagtac acggtgccgt agccggtggc ttccggacgg atcagcgagc
    10081 cgccgaagct cagacccttg ccggtgaata cacagtcgga acggttggtg agcttcttca
    10141 ccatgccgac catgaagccg acctcgcgcc cgcccacgcc gatgtcgccg gccggcacgt
    10201 cggtatccga gccgacatgg cggaacagtt cagcggcgaa cgcctggcag aagcgcatca
    10261 cctcgcccgg gctcttgccc ttcgggtcga aatcggaacc gcccttgccg ccgcccatgg
    10321 gcagcgtggt cagtgcgttc ttgaaggtct gctcgaaggc gaggaacttg aggatcgaca
    10381 ggtttaccga cgggtggaag cgcaggccgc ccttgtaggg accgatcgcc gagctgtgct
    10441 ggatgcggta accgcggttt acctgcactt cgcccttgtc attgacccat gacacgcgga
    10501 actggatcac gcgctcgggt tcgaccagct ggtccagcag cccgtgctcg gcatagcgcg
    10561 gatgcttggc aatgaacggc cacaggctct ccatcacttc caccacggcc tggtggaact
    10621 ccggctggcc ggggttgcgc gctttgacgt agtcgaggaa ttcttccagg ctttggtact
    10681 tcatgacgtc ctttcgcaaa ggggggggaa atgtgcgcgg gacactttca tggtggccgc
    10741 gtcttcggga gtgggatttt gccccaattc gaggcggaaa gatcgtcttt ggcgaacaaa
    10801 tcgcgcactt tttgtgcaca aatgcaccaa aaattggcgg aggggcaacg aacaaacggc
    10861 ctgggcccgg ggtcgaaccg gtttgcgcgc cgccttgtct ttcctgcaag cggaccgcgc
    10921 ctggcctaca cttgcggccc cacgcttttc actccacttc gcgtaatcac ataccgaaca
    10981 tgcccttcac gaccgaaaac caccccggcg tccatcagga tacgcccgcc gccacccgcg
    11041 gcttcaccct gaaccacacg atggtacgcg tcaaggatcc ggcgatctcg ctggatttct
    11101 atacgcgcat tctcggcatg cgcgtgctgc gcaagctcga tttccccgag atgaagttct
    11161 cgctctactt cctcgcgcgc ctggacgaga acgaggcgcc gcccgaggat gtcggcgagc
    11221 gcaccgcctg gaccttcagc cagcgcggca tcctggaact cacccacaac tggggcaccg
    11281 agagcgatgc cgagttccgc taccacgacg gcaacgccca gccgcagggc tacggccaca
    11341 tctgtttttc cgtacccgac ctggctgcgg cgatccgctg gttcgatgag aacaatgtgc
    11401 agtacgtgaa acggcccgag cagggcaaga tgcgcgacgt cgccttcatc aaggatccgg
    11461 acggctactg gatcgaaatc gtccagcccg gcctgttgaa ggcgctcggg cggtcctgag
    11521 gcgagaagcc gcggcgaccg ccgggatcag cgccggctga gcaaagcagt cagccggacc
    11581 cgctggcgaa cggcgccgca atgcgcaaat ccgcaacgaa acgcgcatag gcggccgcct
    11641 tcagctcgcc gggcatgcgc agcaaggcgg atggatgggt ggtgatcaga agctgcctgt
    11701 cttcgccgaa cgacatcagg cggccgcggt tggcctcgat cggcgtggcg cggccgagca
    11761 gcgcgcgcgc cgcggtcgcg cccagggcca ccaccagcgc gggcgccacc aggtccagtt
    11821 cgcgttccag ccactgcacg caggcggcga tctcgacatc gcccggtttc ttgtgcaggc
    11881 gccgcttgcc gcgcagctcg tacttgaagt gcttgaccgc attggtcacg tacacctcgt
    11941 cgcgttcgat gcctgcctcc gccagcgcac ggtcgagcag ccgcccggcg gggccgacga
    12001 acggatggcc ggacaggtct tcctggtcgc caggttgctc gcctacaagc atcacgcgcc
    12061 catgctcggc gccttcgccg aacacggtct gggtggcccc tgcccatagc ggacatgcct
    12121 ggcagccgcg cgcggcggca cgcacgtcat cgagcgaatc ggtttgcggc gcgacgcgcg
    12181 gcggcaatgg tctggctgcg cgcgagcgcg acgcgggacg gccgggagca tgaggtgcgg
    12241 gcatgcagct cagatgcgcg gcccgtgcaa atgttcacct cgctgccgcc cggaacatgc
    12301 agcaccgaac gtgcgcatac cggtattcac accccctgcg atacgtgata atggacgccg
    12361 ccgcagccgc cccggccgcg ctgccgcaca gccacatacg cacagcgggc gcacgacccg
    12421 cgaggagacc acagtggatc atccaggcgc acacccctcg gcgttacctc atggcactct
    12481 tccggcgcag ctgcacagcg caatggtgag gaatgcacac gcgcgctcgg cctcgttcgg
    12541 gctccatacc gccgagaacc ccgagtattc cccgctcacc cgttcggcgc tcggcgccgt
    12601 gatagagcag aaccacgcgc tgcaccagta cgcgctgccg gtgatggaga cgctgtacga
    12661 gcagatcgtc aacacccaca gcatggtgat actgaccgac gcgcaaggcg tgatcatgca
    12721 tgcgctcggc gacgacgatt ttctcgagaa ggcgcgccgc gtggcgcttg cgcccggcgt
    12781 cgcctggtcc gagcagcggc gcggcacgaa tgcaatcgga acggcgattg cggaggccgc
    12841 gccggcactg gtgcatgcgg atgaacacta cctgcgcgcc aaccatgtgc tgacctgctc
    12901 ggccgcgccg atcttcgacg ccgcgggaac gctggccggc gtactcgacg tcaccggcga
    12961 ccgccacagc ttccatccgc acacgatggc gctggtgcgc atgtcggcgc agatgatcga
    13021 gaaccagctg ctcgcggccg cctgcgccga cggcatcacg ctgcgctttc acagccgggc
    13081 agagttcctc ggcacgctga tggaaggcat cgtcacgttc gcgccgggcg gccgctgcat
    13141 cagcgccaac cgcagcgcgc agttccagct cggactggcg ccgacagcgc tgcgcacgca
    13201 cacgctgcaa tcgctgttcg gcatcggggt gtcggccatg gtcgatcatt accgtaccgc
    13261 gatgccggac ccgcttactc ttttcctgcc cagcggcgtg cgcgtgcagg cgcgcgccga
    13321 agtacggctc agccatgccg caagcattgc ggcttccggc gcccgtgcga gcgcgggcgc
    13381 gccgcgccag accgccacag tccgcccccg ctcggcactg catgcgctgg acactggcga
    13441 tccgcagatc gcggccctca tcgcacggct cgagaagatc aaagggcgcg acatccctgt
    13501 cgtcattcac ggcgaaaccg gcaccggcaa ggaactgctc gcccacgcga tacaccgcga
    13561 ttcgccacgc gcggcgggcc ccttcatcgc ggtaaactgc gcggccatcc cggaatcact
    13621 gatcgaatcc gaactgttcg gctacgagga cggcgcattc accggtgcgc gccgcaaggg
    13681 ccatcagggc cgcatcctgc aggccgacgg cggcacgctc tttctcgacg agatcggcga
    13741 catgccgccg gccctgcagg cgcggctgct gcgcgtgctg caggaacgca tggtcactcc
    13801 gctgggaggc agccgctgcg tgccggtcaa cgtggccatc atctgcgcga cgcatcgcaa
    13861 cctgcgcgac atgatgactg ccggcagctt ccgcgaagac ctgtattacc gccttaacgg
    13921 tctcgtcgta aagctgccgc cgctgcgcga gcgccaggac ttcgacgcgg tgatcgggaa
    13981 actgcttgct gccgaggcgc ccgagcgacg ctaccggatc gcgcccgagg tcatggcgca
    14041 gtttcggcgc cacccgtggc cgggcaatct gcgccagctg gccaatctgg ttcgcaccgc
    14101 ggtggtgatg gcgggcgacg acgaagtgat cggccttgcg cacatgccgg acgactttct
    14161 cgatgatctg gcgccgtccg atgtgccggc tgcgcgctgc acgcgcgccc ctgcggccgg
    14221 cagcggacgg ctcgaagatg tcgaagcgca ggcgattcgc gcggcgatcg gcgcgcacgg
    14281 cggcaatgtc tccgccgccg cgcgcgcact gggcatttcg cgcaacacca tctaccgcaa
    14341 gctcaggtag cgtcctgcat cagccgcgca tactcggcgt ccgtgaacag ccgcgagcgc
    14401 gtgagaaagc gcttgccgct accgttctcc agcgagaaca tgccgccgcc cccggggacg
    14461 acgtcgatga tcagctgcgt atgcttccag tattcgtact ggtcgccgcc gatatagaac
    14521 ggcacgccgt ccagatcgcc gaggcgtacg tcggccgcgc cgaccgggaa ctccccctct
    14581 ggaaaacaca tcggcgcgct gccgtcgcag cagccgccgg actggaagaa catcagcttg
    14641 ccatggcgcg ccttcagtgc ggcgatcagt tcgcgcgccg cctcggtggc gagcacgcga
    14701 ggcggaacgg tttcgtcgtt cattgtttct ccggatccgt gtgctgcgtt cggcgttgtc
    14761 atgcatgggc cgagttccaa cgtggctgga ccccggcatt cgccggggtg acgcttgggg
    14821 atgaccggca ccctgcgcct cggcgcacgc cggggcccag cgacgtcatc ccccgcccgc
    14881 ggcacggccc cttcctgcat cagaagaacc cgagcgcctt cgggctgtag ctcaccagca
    14941 ggttcttcgt ctgctgatag tgcgccagca tcatcttgtg gttctcccgc ccgatgccgg
    15001 attgcttgta gccgccgaag gccgcgtgag ccgggtacag gtggtagcag ttggtccata
    15061 cccggccggc ttcgataccc cggcccatgc ggaacgcgcg cgcgccgtcg cgcgtccaca
    15121 cgccggcacc cagtccgtac agcgtgtcgt tggcgatctc cagcgcctcg tcctcgtcct
    15181 tgaacgtggt gaccgacacg accgggccga agatctcttc ctggaagatg cgcatcttgt
    15241 tgtggccctt gaacacggtc ggcttcacgt aatagccctt ctcgaggtcg ccatcgagta
    15301 cgtttcgctc gccgccggcc agcacctgcg cgccttcctg gcggccgatg tcgagatagg
    15361 acaggatctt ctccagctgc tcctgagaag cctgggcgcc gatcatggtc tggctgtcga
    15421 gcggattgcc ctggcggatc gccgccaccc gtgcgagcgc gcgctccatg aagcgatcga
    15481 agatcgattc ctgcaccagc acgcgcgacg gacaggtgca cacctcgccc tggttcagcg
    15541 cgaacatcgc gaagccttcg aggcacttgt cgaaatagtc gtcgtcggcc gccatcacgt
    15601 cctcgaagaa gacgttcggc gacttgccgc ccagctccag tgtgaccgga atgatgttct
//
LOCUS       DATLGF010000120         5101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_145698, whole genome shotgun sequence.
ACCESSION   DATLGF010000120 DATLGF010000000
VERSION     DATLGF010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145698"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /gene="rplQ"
                     /locus_tag="VM406_03575"
     CDS             complement(<1..350)
                     /gene="rplQ"
                     /locus_tag="VM406_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HVL75071.1"
                     /translation="MRHRHGLRKLNRTSSHRLAMLRNMTVSLLRHEAIKTTLPKAKEL
                     RRVVEPILTLGKTDNLSNKRLAFSRLRDREMVVKLFNELGPRYANRNGGYLRILKMGF
                     RQGDNAPMAFVELV"
     gene            complement(481..1458)
                     /gene="rpoA"
                     /locus_tag="VM406_03580"
     CDS             complement(481..1458)
                     /gene="rpoA"
                     /locus_tag="VM406_03580"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HVL75072.1"
                     /translation="MQNSLLKPRIIEVEALGAGHAKVVMEPFERGYGHTLGNALRRVL
                     LSSMVGYAPTEVTIAGVVHEYSSLDGVQEDVVDMLLNLKGVVFKLHNRDEVTLSLKKE
                     GEGTVLASDIDLPHDVELINPDHVIANLTAGGKLDMQIKVEKGRGYVPGNVRRLSEDA
                     NKTIGRIVLDASFSPVRRVSYAVESARVEQRTDLDKLVMNIETNGVISPEEAIRQSAR
                     VLVDQLNVFAALEGTEAAAEAPSRAPAVDPILLRPVDDLELTVRSANCLKAENIYYIG
                     DLIQRSENELLKTPNLGRKSLNEIKEVLASRGLTLGMKLENWPPAGLEK"
     gene            complement(1554..2177)
                     /gene="rpsD"
                     /locus_tag="VM406_03585"
     CDS             complement(1554..2177)
                     /gene="rpsD"
                     /locus_tag="VM406_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HVL75073.1"
                     /translation="MARYIGPKAKLSRREGTDLFLKSARRSLDSKCKLDSKPGQHGRT
                     SGARTSDYGNQLREKQKVKRMYGILERQFRRYFAEADRRKGNTGENLLKLLESRLDNV
                     AYRMGFGSTRAEARQLVSHKAFTVNGEVVNIASYLVKPGDVVAVREKAKKQARILEAL
                     SLAEQSGLPAWVSVDAKKMEGTFKSFPERSEIANDVNESLIVELYSR"
     gene            complement(2336..2740)
                     /gene="rpsK"
                     /locus_tag="VM406_03590"
     CDS             complement(2336..2740)
                     /gene="rpsK"
                     /locus_tag="VM406_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HVL75074.1"
                     /translation="MAKAQSSAAAARARKKVKKNVAEGVAHIHASFNNTIITITDRQG
                     NALSWATSGGAGFKGSRKSTPFAAQVAAEAAGRVAQEYGVKNLEVRIKGPGPGRESAV
                     RALNNLGIKITQIQDVTPVPHNGCRPPKRRRI"
     gene            complement(2783..3148)
                     /gene="rpsM"
                     /locus_tag="VM406_03595"
     CDS             complement(2783..3148)
                     /gene="rpsM"
                     /locus_tag="VM406_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HVL75075.1"
                     /translation="MARIAGVNVPNHQHTVIGLTAIFGIGRPRAQQICEQTGVSTTKK
                     VKDLDDNELEKLRDAIGKFVVEGDLRRQVSMDIKRLMDLGCYRGLRHRKGLPVRGQRT
                     RTNARTRKGPRKAAQALKK"
     gene            complement(3176..3289)
                     /gene="rpmJ"
                     /locus_tag="VM406_03600"
     CDS             complement(3176..3289)
                     /gene="rpmJ"
                     /locus_tag="VM406_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HVL75076.1"
                     /translation="MKVLASVKRICRNCKIIKRKGVVRVICTEPRHKQRQG"
     gene            complement(3319..3537)
                     /gene="infA"
                     /locus_tag="VM406_03605"
     CDS             complement(3319..3537)
                     /gene="infA"
                     /locus_tag="VM406_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HVL75077.1"
                     /translation="MAKDDVIQMQGEVLENLPNATFRVKLENGHVVLGHISGKMRMNY
                     IRILPGDRVTVELTPYDLSRARIVFRTK"
     gene            complement(3562..4896)
                     /gene="secY"
                     /locus_tag="VM406_03610"
                     /pseudo
     CDS             complement(3562..4896)
                     /gene="secY"
                     /locus_tag="VM406_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464914.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
     gene            complement(4927..>5101)
                     /locus_tag="VM406_03615"
     CDS             complement(4927..>5101)
                     /locus_tag="VM406_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uL15 family ribosomal protein"
                     /protein_id="HVL75078.1"
                     /translation="VEEVDMLALKQAGLVPELARAVRVIKAGEITRKVSLKGLVATKG
                     AKAAIEAAGGSVA"
BASE COUNT          959 a         1573 c         1519 g         1050 t
ORIGIN      
        1 tcgaccagct cgacgaacgc catcggcgcg ttgtcgccct gacggaagcc catcttcagg
       61 atgcgcaggt agccgccgtt gcggttcgcg tagcgcgggc cgagttcgtt gaacagcttg
      121 acgaccattt cgcggtcgcg caggcgggag aacgccagac gcttgttcga gaggttgtcg
      181 gtcttgccga gggtgaggat cggctcgacg acgcggcgca gttccttcgc cttcggcagc
      241 gtggtcttga ttgcttcatg acgcagcagc gagacggtca tgttgcgcag catcgcgaga
      301 cggtgggacg aggtacggtt cagcttgcga aggccgtgac gatgacgcat ggtatttcct
      361 ttcgattgag tttgaatcca gctcttctat cgtcaggtat gccctgacgc gggccggtaa
      421 gaatttaacg acagctttgc aaaaacacac cttccccgcg cacggggaag gctacggcaa
      481 ttacttttcc agaccagccg gcggccagtt ctcgagcttc atgcccaggg tcaggccgcg
      541 ggaggcgagc acttccttga tctcgttgag cgatttgcgg cccaggttcg gcgtcttcaa
      601 aagctcgttc tcgctgcgct ggatcaggtc gccgatatag tagatgttct ccgccttcag
      661 gcagttcgcc gaacgcacgg tcagttccag gtcgtcgacc ggacgcagca agatcggatc
      721 cacggccggt gcgcgcgacg gtgcttcggc ggcggcttcc gtgccttcca gcgccgcaaa
      781 aacattcagc tgatcaacca gcacgcgggc ggactggcgg atcgcttcct ccggcgagat
      841 cacgccgttg gtctcgatgt tcatcaccag cttgtcgagg tcggtgcgct gttccacgcg
      901 ggccgattcg accgcgtacg atacacgacg caccggcgag aacgacgcgt ccagcacgat
      961 gcggccgatg gtcttgttcg cgtcttccga caggcgacgc acattgcccg ggacgtagcc
     1021 acggcccttt tcaaccttga tctgcatgtc cagcttgccg ccggcagtca ggttggcgat
     1081 cacgtgatcc ggattgatca gctcgacgtc gtgcggcaga tcgatgtccg aagccagcac
     1141 agtgccctcg ccttccttct tcagcgacag cgtcacttcg tcgcggttgt gcagcttgaa
     1201 caccacgccc ttgaggttca gcagcatgtc gaccacgtct tcctggacgc cgtccagcga
     1261 cgaatattca tgcaccacgc cggcaatcgt tacttccgtg ggcgcataac cgaccatcga
     1321 cgagagcagc acacggcgca gcgcgttccc gagggtgtgg ccgtaaccgc gttcaaacgg
     1381 ctccatcacg actttcgcgt gaccggcacc gagcgcctcg acttcgatga tacggggctt
     1441 caacaagctg ttttgcatgg aatgtccttt tcaataccct cggctcgtta caccgataag
     1501 gctgatggca ttacaaagaa atccgcctcc tggatcagga ggcggggaac caattagcgg
     1561 gaatacagtt cgacgatcag cgattcgttg acgtcgttgg cgatttcgct acgctcgggg
     1621 aaggacttga acgtgccttc catcttcttg gcgtccacgg aaacccacgc cggcagacca
     1681 ctctgctcgg ccagcgacag cgcctcgagg atacgcgcct gcttcttggc cttctcacgc
     1741 acggcaacga cgtcgcccgg cttgaccaga tacgaggcga tgttcacgac ttcgccattg
     1801 acggtgaacg ccttgtggct gaccagctgg cgcgcttcgg cgcgggtcga accgaagccc
     1861 atgcggtagg cgacgttgtc cagacgcgat tcgaggagct tcagcagatt ctcaccggtg
     1921 ttgcccttgc ggcggtcggc ctcggcaaag taacggcgga actggcgttc gaggatgccg
     1981 tacatgcgct tgaccttctg cttttcgcgc agctgattgc cgtagtccga ggtgcgggca
     2041 cccgaggtgc ggccgtgctg acccggcttg gagtcgagct tgcacttgga atccagcgag
     2101 cggcgcgcgc tcttcagaaa caggtcggtg ccttcacggc gggacagttt ggctttgggt
     2161 ccgatataac gtgccacgtt gatttccttt tgttgaatga cgccccagac agccgctcat
     2221 gtacggcgac gactgacggg cgctagtccg gtcctgatgc aaccggacgg tggtcttaag
     2281 aacaaaaccc gccaagcatg ttggcgggcc gctactgcaa cttctgcgac gtcggttaga
     2341 tgcggcggcg cttcggcgga cggcagccgt tgtgcggaac cggcgtcacg tcctgaatct
     2401 gggtgatctt gatgcccagg ttgttcaatg cgcgcacggc cgactcacgg cccgggcccg
     2461 gacccttgat acgcacttcc aggttcttca cgccgtattc ctgagcgaca cggccggcag
     2521 cttcggctgc gacctgcgcg gcgaacggcg tcgacttgcg cgagcccttg aagccagcgc
     2581 cgccggacgt cgcccacgac agcgcgttgc cctggcggtc ggtgatcgtg atgatggtgt
     2641 tgttgaacga ggcgtggatg tgcgcaacac cttcggcgac attcttcttg actttcttgc
     2701 gtgcgcgggc tgctgcagcg ctgctttggg cttttgccat aactctttcc ttgaatccga
     2761 ctattcgaac ggggcgtggc gattatttct tcagcgcctg cgccgccttg cgcggaccct
     2821 tgcgggtacg ggcattggtg cgggtgcgct ggccgcggac cggcaggccc ttgcgatgac
     2881 gcaggccgcg atagcagcca agatccatca ggcgcttgat gtccatcgac acttgacgac
     2941 gcaggtcacc ctcaacgacg aacttgccga tcgcatcgcg cagcttttcc agttcgttat
     3001 cgtccaggtc cttgaccttc ttcgtcgtcg aaacgcccgt ctgttcgcag atctgctgcg
     3061 cgcgcggacg accgattccg aagatcgcgg tcaagccgat aacggtgtgt tggtggttgg
     3121 ggacgttgac ccctgcaata cgtgccattc gttattcctt gatcaataac gttaattaac
     3181 cctggcgctg cttgtggcgg ggttcggtgc aaatcacgcg aacaacgccc ttgcgcttga
     3241 tgatcttgca gttgcggcag atgcgcttga ctgatgcgag cactttcatt tttgccctct
     3301 ttcttgctgt aggttgcttt acttcgtacg gaacacgata cgtgcccggc tcaaatcata
     3361 gggagtcagt tccacggtga ccctgtctcc gggaagaatc cggatgtagt tcatccgcat
     3421 ctttccggag atatggccga gtaccacatg tccgttttcc agcttgactc gaaacgttgc
     3481 attcggaagg ttctcgagaa cctcgccctg catctgaata acgtcgtctt ttgccattcg
     3541 gtacgctcgc tcgtgcgcca atcaacgcat cggaatgccg cccttgaagt tcgccttgcg
     3601 cagcagcgac tcgtactgct gcgacatcac gtagttctgg acctgcgcca tgaagtccat
     3661 ggtcacgacg acgatgatga gcagcgaggt tccaccgaaa tagaacggta cctgccagcg
     3721 ggcaaccagg aattccggca acaggcagac cagcgtaatg tacagggcgc cagccagcgt
     3781 cagacgcatc aggatcttgt cgatgtaccg cgcggtctgc tcccccggac gaatcccggg
     3841 cacgaaggcg ccgctcttct tcagattgtc tgccgtttcc ttggcattga aaaccaatgc
     3901 ggtgtagaaa aagcagaaga agatgatcgc gcccgcatac agcagagcat ggatcggttc
     3961 ccccggcgcc atcgacgcag ccaggtcgcg caggaagcgg accaccggat tcacgtcctc
     4021 accgccggca gtaaaccagc tggtgatcgt tgccgggaac aggatgatcg acgacgcgaa
     4081 gatcggcgga atcacaccag ccatgttcag cttaagcggc aagtggctgc tttgcccgcc
     4141 gtagatcttg ttgcccacct ggcgcttcgc gtagttcacc aggatcttgc gctgaccgcg
     4201 ttcaatgaag accactgcaa acgtgaccgc ggccacgatc gcgcagatca ggatcgccga
     4261 cagcgtgtgc atggcaccag tgcgcaccag ctcgaacaag ccgcccagcg cgctaggcaa
     4321 gcccgcaacg ataccggcaa agatgatgat cgagatgccg ttgccgatgc cgcgctcggt
     4381 gatctgctcg ccgagccaca tcaggaacat cgtgcccgtc accagcgaca ccaccgtggt
     4441 gaagcggaat gcgagaccag ggtcgatcac caggccgggt tgcgattcca gcgtgatcgc
     4501 gattgcgatc gcctgaaata gcgccagaac caacgtgccg tagcgcgtgt actgcgtgat
     4561 cttgcgccgg ccagcctcgc cttccttctt cagtgcctcg agctgcggcg acaccaccga
     4621 cagcagctgc atgatgatcg acgccgaaat gtacggcatg atccccagcg ccaagacgga
     4681 gaaccgctcc agtgcaccgc cctagaacat gttgaacatg ccgagcagtc cgccggactg
     4741 ttggtcgaac agcactgcca gttgcgccgg gtcgattccc ggcaccggaa tgtgggtgcc
     4801 gagacggtag accacgagtg cgcccagcag gaaccaaagg cgtccccaag ggaagccgct
     4861 ggctgcgctc ttagcgagtg gtgaagacgt cgccaattat tgctccgaac aagctgcttg
     4921 ctttgcttag gcgaccgaac cgccggccgc ttcgatggct gccttcgcac ccttggtcgc
     4981 gaccagaccc ttgaggctca ccttgcgggt gatctcgcca gccttgatca cgcgcacggc
     5041 gcgcgccagc tcgggtacca ggcccgcctg cttcagagcc agcatgtcga cctcctcgac
     5101 c
//
LOCUS       DATLGF010000121         8680 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_37475,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000121 DATLGF010000000
VERSION     DATLGF010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 8680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8680
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37475"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VM406_03620"
     CDS             complement(<1..641)
                     /locus_tag="VM406_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HVL75079.1"
                     /translation="MSAVISEANTSQDFFVADLSLADWGRKEIKIAETEMPGLMAIRE
                     EFAAAQPLKGARITGSLHMTIQTAVLIQTLEALGAKVRWASCNIYSTQDHAAAAIAAN
                     GTPVFAFKGENLDEYWDFTHRIFEWPNGEYSNMILDDGGDATLLLHLGARAEKDIGVL
                     DNPDSEEATCLFNAIRKKLATDPTWYSTRLAQIKGVTEETTTGVHRLYDMMKEG"
     regulatory      complement(673..761)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            complement(805..1662)
                     /locus_tag="VM406_03625"
     CDS             complement(805..1662)
                     /locus_tag="VM406_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family murein peptide amidase A"
                     /protein_id="HVL75080.1"
                     /translation="MPAHAGMQQAEWCARLGARLASVTPDLCERSALRPTGAASHEGF
                     PILMRAVPADVRGNAKPVRVMLLGGIHGDELTSVSVVFQWMDLLDQGGARRFEWRIAP
                     VLNPDGLLAPKPQRMNARGVDLNRNFPTPGWERDAKHYWVQMTRSDPRRFPGEAPLSE
                     PESRWLFEEMERFRPDVIISVHAPFGVLDFDGPSSSTPPRQFGRLYLSRVGVYPGSLG
                     NYGGMHKQVPVITIELPNARTMPPKDEVQRIWRDMLAWIDRTLAQEAQPSSGIRRVAA
                     EQARQVEEH"
     gene            complement(1763..2926)
                     /gene="metK"
                     /locus_tag="VM406_03630"
     CDS             complement(1763..2926)
                     /gene="metK"
                     /locus_tag="VM406_03630"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HVL75081.1"
                     /translation="MANQYLFTSESVSEGHPDKVADQISDSILDAILAQDPKARVAAE
                     TLCNTGLVLLAGEITTTANVDYITVARDTLKRIGYNNAEFGIDYNTCCVMVAYDKQSP
                     DIKQGVDEGSGIDLDQGAGDQGLMFGYACNETPELMPAAIYYAHRIVERQSQLRKDGR
                     LPWLRPDAKSQVTLRYVDGKQVSIDTIVLSTQHSPEMQHKAIEEAAIEEIIRPVVPKE
                     WLKNTRYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRS
                     AAYAGRYVAKNIVAAGLAERCQIQVSYAIGVARPTSVMVTTFGTGRISDEQLSELVQE
                     LFDLRPKGIIQMLDLQRPIYAKTAAYGHFGREEPEFSWERTDKVEALRAAARL"
     gene            3141..3983
                     /locus_tag="VM406_03635"
     CDS             3141..3983
                     /locus_tag="VM406_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HVL75082.1"
                     /translation="MLVTLFRLLSRLPLSWLHAMGAGLGWLVYLSSPTYRRRMKQNIA
                     RAGYAQHLRRAIAEAGKNVMELPFVWFASEERLLSAAILEDWDLIERTLAEGRGLIFL
                     TPHLGCFEVTAQVVATRLPMTVLYRPPKKAALKPLLEGARKRGRLSLAPANLSGVRIL
                     LKTLRNGDAVGMLPDQVPQQGEGVWAPFFGKAAYTMTLPAKLQQMTDAPVIIAYGLRV
                     RGGFRIVIERLHEPLSGTTEEQARAVNAAMERLIAHCPEQYFWSYNRYKTPPGVAPAA
                     TEVR"
     gene            3980..4876
                     /locus_tag="VM406_03640"
     CDS             3980..4876
                     /locus_tag="VM406_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HVL75083.1"
                     /translation="MKALLLTLWLLHWLPLPVLGRLGEGIGSVLFAALKKRRDIALTN
                     LRLCMPELPERERRAIARRHFQAYARSVLERSVLWWASEARLRRLIVVEPSMPIEQMT
                     SSPTILLCPHFVCLDVAGAAIAMEISACSMYAEQQNKAFDAVLRRGRSRFKPVKLFTR
                     AEGIKPVIRAMREGLPFFMLPDMDFGAKDAEFVPFFGIPAATLTAPARLAAATGARVI
                     PVIATYLPGYRGWKVRFYPAWEDYPGPDIAAATRRMNAFIEDRVREVPAEYFWAHKRF
                     KTRPPGEPGFYGRAAAPATQTL"
     gene            4884..5735
                     /gene="dapF"
                     /locus_tag="VM406_03645"
     CDS             4884..5735
                     /gene="dapF"
                     /locus_tag="VM406_03645"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HVL75084.1"
                     /translation="MKLKFTKMHGAGNDFIVIDAINQQVGLSPAQWKSLADRRFGIGA
                     DQMLVVERAQAPGVDFRYRIFNADGGEVEQCGNGARAFVKFVSEKGLTDKRTIRVETM
                     AGVIAPTLEDDGRVTVDMGAPILEAERVPFDASGLSGRSEGRDTLWPLELDGGTRWIS
                     VVSMGNPHAVQMVPDSATAPVQQEGPQIESHPRFPRRVNAGFMQIVDRHHIRLRVYER
                     GAGETLACGTGACAAAVAGIRRGLLDTPVRVSTLGGELTIAWNGEDSPVLMTGPAVSV
                     FEGEIEI"
     gene            5820..6488
                     /locus_tag="VM406_03650"
     CDS             5820..6488
                     /locus_tag="VM406_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HVL75085.1"
                     /translation="MNYQPDSQAIADFLLAHPQFFEEHADLLARITLTSPLGGRTVSL
                     QERQMEVLREKNRGLELRLAELMRIAQENDSITDKMQAWTRDLLRARNDVDLPHVLVN
                     GLQTIFGVPHVTLRLWGVSESYAHTWFAAPVSEDARLFCNGLTQPYCGPNQDFEAASW
                     LDEGAEVCSLAMIALRGAEGGPAFGLLVLGSPDPQRFTSEMATDYLAKMGETASAAVA
                     GLLD"
     gene            6494..7450
                     /gene="xerC"
                     /locus_tag="VM406_03655"
     CDS             6494..7450
                     /gene="xerC"
                     /locus_tag="VM406_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HVL75086.1"
                     /translation="MQAEEERAAVAAYLDELRQQRKLSAHTVEGYGRDLAQLLELAKE
                     LPAGADLAKLSHFHIRKFAAQLHSRGLNPRSIARKLSAWRGFYAWYADRVALPANPVD
                     GIKAPRRARPLPKALSADDAVRVVAQARPDRDPNASLQLCNLAMFELLYSSGLRVSEL
                     CGLDVRYVKEGRYVSAGWIDFDECEVNVTGKGSRQRKVPVGQPALQAIRNWLAVRPAL
                     VKADPHPLFLNERGARVSPRVVHARLKAHSRALAIPTNVHPHVLRHSFASHVLQSSGD
                     LRAVQEMLGHASIAATQVYTSLDFQRLAQVYDAAHPRARKKD"
     gene            7475..8677
                     /locus_tag="VM406_03660"
     CDS             7475..8677
                     /locus_tag="VM406_03660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HVL75087.1"
                     /translation="MLTITLKPGKEKSLLRRHPWLFPTAIARVEGKAEERDRPGATAL
                     IQTDTGRFLARAAYSPHSQIRARVWTFEEQEPVDHALIKRRVQQALAYRRAAVHGTDA
                     VRLIFGEADGFPGLVVDWYGGANGYLVGQFQSAGVEAWKAPIVQALTGATACPNVYER
                     SDASVREREGLPLVTGVLAGEAPPDDIEVIENGVRYGVDIKAGHKTGFYIDQRDNRRL
                     VMELAADRDVLNCFCYTGGFSLAALKGGAKSVISIDSAGDALALAQRNVGRNGFDAAR
                     AQWWDADVFKTLRRLREEGRRFDLIVLDPPKFASSPGQVERAARAYKDINLLGFQLLR
                     PGGLLFTYSCSGAISADLFQKIVAGAANDAAVDARILRRLSAGVDHPMTTSFPEGEYL
                     KGLLLQRP"
BASE COUNT         1430 a         2897 c         2823 g         1530 t
ORIGIN      
        1 ccctccttca tcatgtcgta gagccggtgg acgccggtgg tggtctcctc ggtgacgccc
       61 ttgatctggg caaggcgggt cgaataccag gtcggatcgg tcgcaagctt cttgcggatc
      121 gcattgaaca ggcaggtcgc ctcttccgaa tcggggttgt cgagcacgcc gatgtccttc
      181 tctgcgcgcg cgcccaggtg cagcagcagc gtcgcgtcgc cgccgtcgtc gaggatcatg
      241 ttcgagtatt cgccgttcgg ccactcgaag atgcggtgcg tgaagtccca gtactcatcg
      301 aggttctcgc ccttgaatgc gaacaccggc gtgccgttgg ccgcgatcgc ggcggcggcg
      361 tggtcctggg tcgagtagat gttgcacgag gcccagcgca ccttggcgcc cagcgcttcc
      421 agcgtctgga tcagcaccgc ggtctggatc gtcatgtgca gcgaaccggt gatgcgcgcg
      481 cccttgagcg gctgggcggc ggcaaactcc tcgcggatcg ccatcaggcc cggcatttcg
      541 gtctcggcga tcttgatctc cttgcggccc cagtcggcca gcgagagatc ggcaacgaag
      601 aagtcttggg aagtattggc ttctgaaatc acggcgctca tcacgccctc ctttccagtg
      661 aataagaaaa aagtaacgtg agcgccgttg cgacaggggg atgccccggg atccaagcct
      721 gacccggtct gaacagccgg gctgcaacgc tccttggaat cctgaattat agccgacagg
      781 gcaagcgccg ccagcgggaa aacctcaatg ctcttcgact tgacgcgcct gctcggccgc
      841 cacgcggcgg atgccggacg aaggctgcgc ctcctgcgcc agcgtgcggt ctatccacgc
      901 gagcatgtcg cgccagatgc gctgcacctc atccttcggc ggcatcgtgc gcgcattcgg
      961 cagctcgatc gtgatcaccg gcacctgctt gtgcatgccg ccgtagttgc ccagcgatcc
     1021 cggatagacg cccacccggc tcaggtacag ccggccgaac tggcgcggcg gcgtgctgga
     1081 actcggaccg tcgaaatcta gcacgccgaa cggcgcatgc accgagatga tcacgtccgg
     1141 acgaaagcgc tccatctctt caaacagcca gcgcgattcc ggttccgaca gcggcgcctc
     1201 cccggggaag cggcgcggat cgctgcgcgt catctgcacc cagtagtgct tggcgtcgcg
     1261 ctcccatccg ggtgtcggga agttgcggtt cagatcgaca ccgcgtgcat tcatgcgctg
     1321 cggcttgggc gcgagcaggc catccggatt cagcaccggt gcgatgcgcc attcgaaacg
     1381 gcgcgcaccc ccctgatcga gcaggtccat ccactggaac acgaccgaca ccgaggtgag
     1441 ttcgtcacca tggatgccgc caaggagcat gacgcgcacc ggcttcgcgt tgccgcgcac
     1501 gtcggccgga accgcgcgca tcaggatcgg aaacccctcg tgggaagcgg cgccggtggg
     1561 gcgcagcgcg ctgcgttcgc acaggtccgg cgtaacgctc gccaggcgcg cgcccaggcg
     1621 tgcgcaccac tccgcctgct gcatgccggc gtgcgccggc acggcacagg cgatcccgat
     1681 ccaggccgca agccctgcgg cgacactacg aagcacttgc aaaacccgtc atcctcccga
     1741 ttcgcgccgc agtcgctccg ccttacagcc gtgcggccgc gcgcagcgct tcaaccttgt
     1801 cggtgcgctc ccagctgaac tcgggctcct cacggccgaa gtggccgtag gcggcggtct
     1861 tcgcgtagat cggacgctgc aggtcgagca tctgaatgat gcccttcggg cgcaggtcga
     1921 acagctcctg caccagttcg gacagctgtt cgtcgctgat ccggccggtg ccgaaagtcg
     1981 tcaccatcac cgaggtcggg cgcgccacgc cgatcgcgta cgacacctga atctggcagc
     2041 gctcggccag gccggcggcg acgatattct tcgccacgta gcgcccggca tatgcggccg
     2101 agcggtcgac cttggtcgga tccttgccgg agaatgcgcc accgccgtgc ggcgcggcgc
     2161 cgccgtaggt gtcgacgatg atcttgcgcc cggtcagacc gcagtcgcct tgcggaccgc
     2221 cgatcacgaa gcgaccggtc ggattgatca ggtagcgggt gttcttcagc cattccttcg
     2281 gcacgaccgg acggatgatt tcctcgatcg ccgcttcctc gatcgccttg tgctgcatct
     2341 cggggctgtg ctgggtcgac agcacgatgg tgtcgatcga gacctgcttg ccgtcgacgt
     2401 agcgcagcgt gacttgcgac ttcgcatccg gacgcagcca gggcaggcgg ccatccttgc
     2461 gcagctgcga ctggcgctcg acgatgcggt gcgcatagta gatcgccgcc ggcatcagct
     2521 cgggcgtttc attgcacgcg tagccgaaca tcaggccctg gtcgccggcg ccctggtcaa
     2581 ggtcgatgcc gctgccttcg tcgacaccct gcttgatgtc gggcgactgc ttgtcgtacg
     2641 cgaccatcac gcagcaggtg ttgtagtcga tgccgaactc ggcgttgttg tagccgatgc
     2701 gcttcaaggt gtcgcgcgcg accgtgatgt agtcgacgtt ggcggtcgtg gtgatttcgc
     2761 cggccagcag caccagtccg gtgttgcaca gcgtttcggc ggccacgcgc gccttcggat
     2821 cctgggcgag gattgcgtcg aggatggagt ccgagatctg gtccgcgacc ttgtccggat
     2881 gaccttcgga gacggattcg gaagtgaaaa gatattgatt tgccatcgca ggctcctgtt
     2941 gttgttgacc cctgtcgcgg ccaatcccat tgtgcctgcg acgctttagc gagattttta
     3001 ttccgcctcg caagttgtct gattaactcg gcggaccggt ttcatcggca tgcggcgaac
     3061 ctatgcctcc ctgcgacgaa accttggtgt gctattttac gcgtctgcaa atttttgaca
     3121 aacgttccgc acgctctttg atgctggtca ctctgtttcg cctgctgtca agattgccat
     3181 tgtcctggct gcacgcgatg ggcgcaggcc tgggctggct ggtctaccta tcttccccca
     3241 cctaccgacg tcgcatgaag cagaacattg cgcgcgcagg atatgcgcaa catctgcgtc
     3301 gcgcgatcgc agaagcggga aagaacgtga tggaactgcc gttcgtgtgg ttcgcgtccg
     3361 aagagcggct gttgtccgcg gccatcctcg aagactggga cctgatcgag cgcacgctgg
     3421 ccgaaggccg cggcctgatc ttcctcacgc cccatctcgg ctgcttcgag gtgactgccc
     3481 aggtcgtcgc cacgaggctg ccgatgacgg ttctctatcg cccgccaaag aaggcggccc
     3541 taaagccgct gctcgaaggc gcgcgcaagc gcggccggct atcgcttgca ccggcaaatc
     3601 tatccggcgt gcgcattctg ctcaagacgc tcaggaacgg cgatgcggtg ggcatgctgc
     3661 cggaccaggt gccgcagcag ggcgaaggcg tatgggcacc tttcttcggc aaagctgcct
     3721 atacgatgac gctgccggcc aagctgcagc agatgacgga cgcgcccgtc atcatcgcct
     3781 acggcctgcg cgtacgcggt ggcttccgca tcgtgatcga gcgcctgcat gagccgctgt
     3841 ccggtacaac cgaggaacag gcgcgcgcgg tcaatgccgc gatggagcgg ctgatcgcgc
     3901 attgccccga acagtatttc tggagctata accgctacaa gaccccgccg ggagtcgctc
     3961 ccgccgcgac agaggttcga tgaaagcatt gctattgaca ctatggctgt tgcactggct
     4021 gccgttgccg gtgctgggac ggctcggaga aggcataggc agcgtgctgt tcgccgcgct
     4081 gaagaagcgg cgcgacatcg cgctgaccaa cctgcggctg tgcatgccgg aactgcccga
     4141 acgcgaacgc cgcgcgatcg cgcgccgcca cttccaggcg tatgcgcgca gcgtcctcga
     4201 acgcagcgtg ctgtggtggg catccgaggc acgcctgcgc cggctgatcg tggtcgaacc
     4261 gtcgatgccg atcgagcaga tgacctcgag tcctaccatc ctgctgtgcc cgcactttgt
     4321 ctgcctcgac gtggccggcg cggcgatcgc aatggaaatc tcggcctgtt cgatgtatgc
     4381 ggagcagcag aacaaggcgt tcgacgcggt gttgcggcgc ggccgctcgc gcttcaagcc
     4441 ggtcaagctg tttacccgcg ccgagggcat caagccggtg attcgcgcga tgcgcgaagg
     4501 gctgccgttc ttcatgctgc cggacatgga ctttggcgcg aaggatgccg agttcgtccc
     4561 cttcttcggc atccccgccg ccaccctgac cgcgccggcg cggctcgcgg cggccaccgg
     4621 cgcaagagtg atcccggtga tcgcgaccta cctgcccggc tatcgcggct ggaaggtgcg
     4681 cttctacccg gcctgggaag actatccggg gccggacatc gccgcggcca cgcgccgcat
     4741 gaacgccttc atcgaggacc gggtgcgcga ggtgccggcc gaatacttct gggcgcacaa
     4801 gcgcttcaag acacgtccgc ccggcgagcc cggcttttat ggacgcgccg ccgccccggc
     4861 aacgcaaacc ctataatgcc gcgatgaagc tcaagttcac gaaaatgcac ggcgccggca
     4921 acgatttcat cgtcatcgac gcgatcaacc agcaggtggg gctcagcccc gcgcaatgga
     4981 agtcgctcgc cgaccgccgc ttcggcatcg gggccgacca gatgctcgtg gtcgagcgtg
     5041 cgcaggcgcc cggcgtcgat ttccgctatc gcatcttcaa tgccgacggc ggcgaggtcg
     5101 agcagtgcgg caatggcgcg cgcgcgttcg tgaagttcgt cagcgaaaag ggtttgacgg
     5161 acaagcgcac gatacgtgtc gaaaccatgg cgggcgtgat cgcgccgacg ctggaagacg
     5221 acgggcgggt gaccgtcgac atgggcgcgc cgattctcga agccgaacgc gtaccgttcg
     5281 acgcttccgg cctgtcaggc cgaagcgagg ggcgcgatac gctttggccg ctcgagctcg
     5341 atggcggcac gcgctggatc tcggtggtct cgatgggcaa tccgcacgcg gtgcagatgg
     5401 tgcccgattc cgccactgcg ccagttcagc aggaaggccc gcagatcgaa agccatcccc
     5461 gctttccgcg ccgagtcaat gccggcttca tgcagatcgt cgaccgccac catatccggc
     5521 tgcgcgtcta cgagcgtggc gccggcgaga cgctggcctg cggcaccgga gcctgcgcgg
     5581 ccgcggtggc cggcattcgc cgcggcctgc tcgatacgcc ggtgcgcgtc tcgacgctgg
     5641 gcggtgagct gacgatcgcc tggaacgggg aagacagtcc ggtgctgatg accggtcccg
     5701 ccgtctcggt attcgaaggc gaaatcgaga tttgacgaca tccccgcccc ttcggcgttg
     5761 ccggctcggg tagaatcacg ggttgattcc aggcatcacc acaccccacg caccccgcca
     5821 tgaactacca accggactcc caggccattg ccgacttcct gctcgcgcat ccgcagttct
     5881 tcgaggagca tgcggacctg ctggcgcgca tcacgctgac cagcccgttg ggcgggcgca
     5941 cggtttcgct gcaggagcgg cagatggagg tgctgcgcga aaagaaccgc gggctggagc
     6001 tgcgcctggc ggaactcatg cgcatcgccc aggaaaacga cagcatcacc gacaagatgc
     6061 aggcctggac gcgcgacctg ctgcgcgccc gcaacgatgt cgatctgccg cacgtcctgg
     6121 tcaatggcct gcagacgatc ttcggcgtgc cgcacgttac gctgcgcctg tggggcgtct
     6181 cggagagcta cgcccatacc tggttcgcgg cgccggtgtc ggaggatgcg cggctgtttt
     6241 gcaacgggct gacccagccc tactgcggcc cgaaccagga tttcgaagct gcatcctggc
     6301 tggacgaggg cgccgaggtg tgttcgctgg cgatgatcgc gctgcgcggc gccgagggcg
     6361 gcccggcgtt cggcctcctg gtgctcggtt cgcccgatcc gcagcgcttc acttccgaga
     6421 tggcgaccga ctatctcgcc aagatgggcg agacagccag cgccgcagtc gccggcctgc
     6481 tcgactgagt gcaatgcagg cggaggagga gcgcgccgcc gttgccgcct acctggacga
     6541 actgcggcag caacgcaagc tgtccgcaca taccgtcgag ggctacgggc gggacctggc
     6601 gcaactgctg gagctggcga aagaacttcc cgccggtgct gacctcgcca agctgtccca
     6661 ctttcatatc cgcaagttcg ccgcgcaatt gcactcgcgc ggcctgaacc cgcgctcgat
     6721 cgcgcgcaag ctgtccgcct ggcgcggctt ctatgcctgg tatgctgacc gtgtcgcctt
     6781 acccgccaat ccggtcgacg ggatcaaggc gccccgtcgc gccaggccgc tgccgaaggc
     6841 cttgtctgcg gacgacgcgg ttcgcgtggt agcgcaagcg cggccggacc gcgacccgaa
     6901 cgcgtcgctc cagctgtgca acctggcgat gttcgaactg ctgtactcga gcgggctacg
     6961 cgtatccgaa ctgtgcggac tcgacgtgcg ttacgtgaag gaaggccgct acgtatcggc
     7021 cggctggatc gacttcgacg aatgcgaagt caatgtgacc ggcaagggca gccgccagcg
     7081 caaggtgccg gtcggccagc cggcgctgca ggcgatccgc aactggctgg ccgtgcggcc
     7141 ggcgctggtc aaggccgatc cccatccgct gttcctgaac gagcgcggcg cgcgggtttc
     7201 gccgcgggtg gtccatgcac gcctgaaagc ccactcccgc gcactggcga tcccgactaa
     7261 cgtccatccg cacgtgctgc gacactcgtt cgcctcccac gtgctgcaat cctcgggcga
     7321 cctgcgcgcg gtgcaggaaa tgctgggcca cgcgtcgatc gccgcgacgc aggtctatac
     7381 ttccctggac ttccagcgcc tggcccaggt ctatgacgcg gcccatccgc gcgccaggaa
     7441 gaaagactga cggttttccc ctcttccgta tcccatgctc accatcaccc tcaagccagg
     7501 caaggaaaaa agcctgctgc gccgccatcc gtggttattt cccaccgcca tcgcccgtgt
     7561 tgaaggcaag gccgaggaac gcgaccggcc cggcgcgacc gcgctgatcc agacagatac
     7621 cgggcgcttc ctcgcgcgtg ccgcctacag cccgcactcg cagatccgcg cgcgcgtctg
     7681 gactttcgag gagcaggagc cggtcgacca cgcactgatc aagcgccgcg tgcaacaggc
     7741 tctggcctac cgtcgcgcgg ccgtgcacgg caccgacgcg gtgcggctga tcttcggcga
     7801 ggccgacggc tttcccggac tggtggtgga ctggtacggc ggcgcaaacg gatatctcgt
     7861 cggccagttc cagtccgccg gcgtcgaggc ctggaaggcg ccgatcgttc aggcgctgac
     7921 cggcgcgacc gcctgcccga acgtctacga gcgttccgac gcctcggtgc gcgagcgcga
     7981 gggtctgccg ctggtcaccg gcgtgctggc cggggaggcg cctccggatg acatcgaagt
     8041 gatcgagaac ggcgtgcgct acggcgtcga catcaaggcc ggccacaaga ccggtttcta
     8101 catcgaccag cgcgacaacc ggcggctggt gatggagttg gccgcggacc gcgacgtcct
     8161 caactgcttt tgctataccg gtggcttttc gctggcggcg ctcaaaggcg gagcgaaaag
     8221 cgtgatctcg atcgattcgg ccggcgacgc gctcgcgctc gcgcagcgca acgtcgggcg
     8281 caacgggttc gacgccgcac gggcgcaatg gtgggatgcc gacgtcttca agaccctgcg
     8341 ccgcctgcgc gaggaaggcc ggcgcttcga cctgatcgtg ctcgatccgc ccaagttcgc
     8401 ctcgtcgccc ggtcaggtgg agcgcgccgc gcgcgcatac aaggacatca atctgctcgg
     8461 tttccagctg cttcgccccg gcggcctgtt gtttacctac tcctgctcgg gtgcgatcag
     8521 cgccgacctg ttccagaaga tcgtcgccgg cgcggccaac gatgccgccg tcgacgcgcg
     8581 catcctgcgc cggctgtcgg cgggcgtcga tcacccgatg accacctcgt ttccggaagg
     8641 cgagtacctg aagggcctgc tgctgcagcg tccctgatgt
//
LOCUS       DATLGF010000122         8013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_113895, whole genome shotgun sequence.
ACCESSION   DATLGF010000122 DATLGF010000000
VERSION     DATLGF010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 8013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8013
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113895"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1104)
                     /locus_tag="VM406_03665"
     CDS             complement(184..1104)
                     /locus_tag="VM406_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75088.1"
                     /translation="MPDYNSRYPDGLHRRFIPEETPVMRKPAAAPQPVDPALLPQRRP
                     IFTAVRWGAVLAGVAVGLSMQLVITLLGIAAGLTAGDVVGRERFGIGPLLWAALSMLV
                     AAFAGGYVAARMSGLKRTADGILHGAVSWAVTTILFAVLATSIGGALVGGAMAGGVFT
                     SMAQLAQAQEQVAQGEGPVATFMRQQVDEMDVISLSLFRQYIRSGEREAAVSLLIETT
                     SLEPGRAEAVVDQALILSGAPEAASPGVRKNTEVAVQGAGLAAWIVFGAVALGLALGM
                     AGGRLGVAGARRVNWSDRQGEPPPEEDANR"
     gene            complement(1150..1608)
                     /locus_tag="VM406_03670"
     CDS             complement(1150..1608)
                     /locus_tag="VM406_03670"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HVL75089.1"
                     /translation="MWNRQFKGAVIGVCILGLAACAAPQQSAQQGVPAAGATTSATGY
                     GVVQAIDIVPREQTGTGVGTVAGAVVGGALGSQVGGGSGRTAATIAGAAGGALAGGAL
                     TRGTHPGHVYRVAILMDNGSVQTMVQETPPGLQVGERVRLSNGVVVERYR"
     gene            1878..3173
                     /locus_tag="VM406_03675"
     CDS             1878..3173
                     /locus_tag="VM406_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013094097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HVL75090.1"
                     /translation="MRKIALISEHASPIARLGSVDSGGQNVYVAQLARHLARQGYQVD
                     VFTRRDSPALATLVHWQPGVRIIHVPAGPAEYIPKEAMLPYMEEFGRFMIRFAQRQKD
                     PYDIVHANFFMSGMVAQQMKKALGIPFVMTFHALGRVRRLNQKEADGFPDLRFAIEER
                     LIREADRIVAECPQDKQDMEDLYGACPEHIDIVPCGFDPEEFGTVTPDARGHLGLARD
                     EFIVLQLGRMVPRKGVDNVIRATAMLRHQYQVPARLLVVGGNSAQPDPDATPELGRLM
                     RLTHELGIDEAVTFTGQRQREQLRYYYSAADVFVTTPWYEPFGITPLEAMACATPVVG
                     SAVGGIKYTVVDGETGYLVPPNDPASLAERLAWLHAHPQLAQRLGWAGMRRAYQLFTW
                     RSVAASIAQVYENALASQPLWQARVVGAEMPSSAAGAAS"
     gene            3265..4032
                     /locus_tag="VM406_03680"
     CDS             3265..4032
                     /locus_tag="VM406_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL75091.1"
                     /translation="MSPTQVSSYVADPAESRHLAGKTILVTGGASGLGAALSHVLVSS
                     GANVVIGDLNLDKAQTVAALLEDAPGKAHALELNVGDPASAERAVEDSVERFGQLDIL
                     INNAGTDVTCSIDEMDIGDWDRVMRTNLYGPFLLSKFAARKMKANGGQIVNIASTAAK
                     RAWPNASAYHASKWGLLGLSHALHAELRSQRIKVTAVLAGGMRTPFLLDRFPDIDVST
                     LQDPVNVARAVKSVLMLPEQTVIPEVMVIPMTESSWP"
     gene            4038..4637
                     /locus_tag="VM406_03685"
     CDS             4038..4637
                     /locus_tag="VM406_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HVL75092.1"
                     /translation="MRAIFLDKDGTLIEDVPYNVDPALITLSWQAGPALQLLQHLGYK
                     LIVVTNQSGVARGLFTEAALLPVRRRLDALLAQYGVRLDGFYYCPHSPQGVVERYAVS
                     CTCRKPMPGLLTRAAHEHGLDLGQSWMIGDILNDVEAGRRAGCRTVLIDNGNETEWER
                     SPLRVPDLVAPDLHTAAGLIAAAAAADAAAPQLGGAGSP"
     gene            4634..5728
                     /gene="waaF"
                     /locus_tag="VM406_03690"
     CDS             4634..5728
                     /gene="waaF"
                     /locus_tag="VM406_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HVL75093.1"
                     /translation="MSTSSDRWQQARNILAIRLDYLGDVLMTTPALRALRNRAGGRIT
                     LLTSPGGAAVAPCIPEIDDTIVYAAPWLKHSEAPDAARDLAMIETLRARRFDAAVIFT
                     VYSQNPLPAALLCHLAGIPLRLAHCRENPYQLLSDWVAETEPQQQVRHEVRRQLDLVA
                     TVGARCEDERLSFCVPDTAQEAVRAHLARLHVRKRFIVLHPGASAASRRYPAASFRRA
                     AELLSRNFRHPLVFTGSTAEAPLIDEIRAGLPDAHSLAGRLSLAELGALLREAALVVT
                     NNTGPAHLAAAIGTPVVDLYALTNPQHTPWQVPARVLFQDVSCRYCYRSACPMGHQAC
                     LASVAPERVLEAAAELLGGARDSRPALPPL"
     gene            5974..6480
                     /locus_tag="VM406_03695"
     CDS             5974..6480
                     /locus_tag="VM406_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HVL75094.1"
                     /translation="MRLLAEDHKKVKKMFDEFEKIKDKEDIDDEQKQLLVETACAELT
                     IHAQLEEELFYPAARDAIDDMDLLDEAEVEHASAKQLISELATMQPGDALYNAKFKVL
                     GEYVKHHIEEEEKELFPLIKKSELDLAELAEEMQERRMELREELGFTAVETDEDAEAA
                     PKARKNVH"
     gene            6509..7093
                     /locus_tag="VM406_03700"
     CDS             6509..7093
                     /locus_tag="VM406_03700"
                     /inference="COORDINATES: protein motif:HMM:NF014755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HVL75095.1"
                     /translation="MTLQQEPVPAASSLTHAVDILAFVEAQEIPPDYFETPYFLAPEP
                     GGEKDYALLRETLRRTRKIGIAYVVIQARQHLAALIPHGQSLVLNTLRWNTEDRERAR
                     TAREAVAVADPARAPIERGTTPPLQRMRVTGCEDFDPDDLEELLEESLFDCDDVIASL
                     MRPRLHYSVPPAGRGTTLRQPLGAARPRRFARRA"
     gene            complement(7193..7690)
                     /locus_tag="VM406_03705"
     CDS             complement(7193..7690)
                     /locus_tag="VM406_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HVL75096.1"
                     /translation="MSALLAMPDWGDLFSLTVPLTEIIARGSIMYCFLFMLFRFVIRR
                     DVGAVGIADLLVLVIVADAAQNGMAGDSTSLADGMALVATLIGWNVLLDWLAFRFAFV
                     RRFAEPKPIKLAERGRLLPRNMRREFISEDELWSKLRQSGVESLDQVKVVYMEADGQI
                     SVIKA"
     gene            complement(7772..>8013)
                     /locus_tag="VM406_03710"
     CDS             complement(7772..>8013)
                     /locus_tag="VM406_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75097.1"
                     /translation="YVLNADRPTIREAPGFDMARWPDLGDPHWRATVDGYWARRTGHA
                     VGLHNDPLSAPAATTGTGQGSGAARGGAPPPERAR"
BASE COUNT         1390 a         2772 c         2584 g         1267 t
ORIGIN      
        1 gtgctgtggg gcagcgtggt ggtggacggc atcgtggtcg cggtcagcgg cgccaacccc
       61 tggtacgacg aagccttcgc cggcaccgtg gcgatgtgcc tgaaggcgat cgcgaaagcg
      121 ggcatcgtgg aaaagcgcgc cggcgggctg ttcctccccg cgaagtagag gcaagcgcgc
      181 cggctaccgg tttgcatctt cctccggcgg cggctcgccc tgccggtccg accagttgac
      241 ccggcgcgcg ccggccacgc cgaggcgccc gccggccatg ccgagcgcaa gtcccagggc
      301 gaccgcgccg aacacgatcc aggcggccag tcccgcaccc tgcactgcca cctcggtgtt
      361 cttcctgact ccgggcgagg ccgcctccgg ggcgccggac aggatcagcg cctgatccac
      421 cacagcctcc gcgcgaccag gctccagcga tgtggtctcg atcagcaggc tcaccgcggc
      481 ctcgcgctcg ccgctgcgga tgtattggcg gaacaggctc aggctgatca catccatctc
      541 gtcgacctgc tggcgcatga aggtggccac cgggccttcg ccctgcgcca cctgttcctg
      601 cgcctgggcg agctgggcca tgctggtgaa cacgccgccg gccatcgccc cgccgaccag
      661 ggcgccgcct atcgaggttg ccaggaccgc gaacaggatg gtggtgaccg cccatgacac
      721 cgcgccatgc aggatgccgt cggcggtacg cttcaacccg gacatgcgcg cggcgacata
      781 accgccggcg aatgccgcca ccagcatgct caacgccgcc cacagcagcg ggccgatgcc
      841 gaagcgctca cggccgacca cgtcgccggc ggtcaggccg gcggcgatgc cgagcagcgt
      901 gatgacaagc tgcatcgata gcccgaccgc gacgccggcg agaacggcgc cccagcgcac
      961 cgcggtgaag atcggccggc gctgcggcaa gagcgcggga tcgaccggct ggggcgctgc
     1021 ggccggcttg cgcatgaccg gcgtttcctc cggaatgaaa cgccggtgca ggccgtcggg
     1081 gtagcggctg ttgtagtcag gcatctgcgc gtttttccgt cagaccaaaa agatgcgccc
     1141 ggccccgcat cagcgatagc gctcgacgac cactccattg gacaagcgca cgcgctcgcc
     1201 gacctgcaga ccgggcggtg tttcctgcac catcgtctgc accgaaccgt tgtccatcag
     1261 gatcgcaacg cgatagacgt gtccgggatg ggtgccgcgc gtcagcgctc cgcccgcaag
     1321 cgcgccgccg gccgctccgg cgatcgtggc ggcagtgcgt ccggacccgc cgccgacctg
     1381 gcttccgagc gcgccgccga cgacggcccc cgccacggtg ccgaccccgg tgcccgtctg
     1441 ctcgcgcgga acgatgtcga tcgcctgaac cacgccatag ccggtcgcac tcgttgtcgc
     1501 gccggccgcc ggtacgccct gctgcgcgct ctgctggggt gctgcgcagg ccgcgaggcc
     1561 aagtatgcag acgccgatca cggcgccctt gaactgtctg ttccacatag gaagctcctt
     1621 ttcatccagg cacggcgccg gcacgcggcg cctgtcacgt ttccccggcg atgcagggaa
     1681 catgccagtc agcgcggcaa cacaggcgtt gcagcaccgc cgcagggtcg tgaaaagctt
     1741 gcagccgccg gtaggaatta ctcccggtct ggcgaggtat gccgcggcaa cttcgcgcgt
     1801 tacgagcagt ggcccccggg tacgttgatt gctttgcaac ctctcacgag ttctcgccct
     1861 tttcctacgt ataacttatg agaaaaattg cactgatcag cgagcacgct tcgcccatcg
     1921 cgcggctcgg cagcgtcgac agcggcggtc agaacgtcta tgtcgcccag ctcgcccgcc
     1981 atctggcacg ccagggctac caggtcgacg tcttcacgcg ccgcgacagc cccgcccttg
     2041 ccaccctcgt ccactggcag ccgggcgtgc gcatcattca cgtccccgcc ggccccgccg
     2101 aatacattcc caaggaagcg atgctgccgt acatggagga gttcggccgc ttcatgatcc
     2161 gcttcgcgca gcgccagaag gatccatacg acatcgtgca cgccaacttc ttcatgtccg
     2221 gcatggtcgc gcagcagatg aagaaagcgc tcgggattcc gttcgtgatg accttccatg
     2281 cgctcgggcg cgtacgccgc ctcaaccaga aggaagccga cggctttccg gacctgcgct
     2341 ttgcaatcga agagcgcctg atccgcgaag ccgaccgcat cgtcgccgag tgtccgcaag
     2401 acaagcagga catggaagat ttgtacggcg cctgccctga acatatcgac atcgtcccct
     2461 gcggtttcga tcccgaggaa ttcggcacgg tcactcccga cgcccgcggc catctcgggc
     2521 tcgcacgcga cgagttcatc gtgctgcagc tcggccgcat ggtcccccgc aagggggtgg
     2581 acaacgtgat ccgcgccacc gccatgctgc gccaccagta tcaggtgccg gcgcgcctgc
     2641 tcgtcgtcgg cggcaactcg gcacagccgg acccggatgc cacgcctgaa ctcgggcggc
     2701 tcatgcggct gacgcacgag ctgggcatcg acgaggcagt caccttcacc gggcagcgcc
     2761 agcgcgagca gctgcgctat tactacagcg cggccgatgt gttcgtgacg acgccctggt
     2821 acgagccttt cggcatcacg ccgctggaag cgatggcctg cgcgacgccg gtggtcggca
     2881 gcgcggtagg cggaatcaag tacacggtgg tcgacggcga aaccggctac ctggtgccgc
     2941 cgaacgaccc ggcatcgctg gccgaacgcc ttgcatggct gcatgcgcat ccgcagctcg
     3001 cgcagcgcct gggctgggcc ggcatgcgcc gcgcctacca gctgtttacc tggcgcagcg
     3061 tcgccgccag catcgcgcag gtttatgaaa atgcgctggc gtcccagcct ctgtggcagg
     3121 cacgggtggt cggcgccgag atgccgtcga gcgccgccgg cgccgcctct tgattccggc
     3181 ccggggcatc actccatttt catcaaggag cccgcatcga tgcccgcgta gccttgcagc
     3241 tgaactttca cactggagaa ggaattgagc cccacccaag tatcaagcta tgtagctgac
     3301 ccggccgagt cccggcacct ggccggcaag accatactcg tcaccggcgg cgccagcgga
     3361 ctcggcgcgg ccctgtcgca tgtgctggta tcttcgggcg ccaatgtcgt catcggcgac
     3421 ctcaacctgg acaaggcgca gaccgtggcc gccttgctgg aggacgcgcc gggcaaggcg
     3481 catgcactgg aactcaacgt cggcgatccg gcatcggccg agcgtgcggt cgaagacagc
     3541 gtcgagcggt tcggccagct cgacatcctg atcaacaatg ccggcaccga cgtcacctgc
     3601 tcgatcgacg agatggacat cggcgactgg gaccgcgtga tgcgcaccaa cctctacggc
     3661 ccgttcctgc tgtcgaagtt tgcggcgcgg aagatgaagg ccaacggggg acagatcgtc
     3721 aacattgctt ccaccgccgc caagcgcgcg tggccgaacg cctccgccta tcacgcaagc
     3781 aagtggggcc tgctcggact gtcgcacgcg ctgcatgcgg aactgcgatc gcagcgcatc
     3841 aaggtcacgg cggtgctggc cggcggcatg cgcactccgt tcctgctcga ccgcttcccg
     3901 gacatcgacg tctccaccct gcaggatccg gtgaatgtcg cgcgcgcggt caagtcggtg
     3961 ctcatgctgc ccgagcagac cgtgatcccc gaggtcatgg tgatcccgat gaccgaaagc
     4021 tcctggccct gagcttcatg cgcgcgatct tcctcgacaa ggacggcact ctcatcgagg
     4081 atgtcccgta caacgtcgac ccggcgctga tcacgctgag ctggcaggcc ggcccggcgc
     4141 tgcagctgct ccagcatctg ggctacaagc tgatcgtcgt gaccaaccag tcgggcgtgg
     4201 cgcgcggcct gttcaccgag gcggcactgt tgccggtgcg gcggcggctc gatgcgctgc
     4261 tggcccagta cggggtcagg ctcgacggct tctactactg cccccacagc ccgcagggcg
     4321 tggtggagcg ttatgcggtc tcctgcacct gccgcaagcc gatgcccggt ctgctcaccc
     4381 gtgctgcgca cgagcacggg ctggatcttg gccagtcatg gatgatcggc gacatactca
     4441 acgacgtcga agcgggaagg cgcgccggtt gccgcacagt gctgatcgac aacggcaatg
     4501 agaccgaatg ggagcgctcg ccgctgcgcg tgccggatct cgtcgcgccc gacctgcaca
     4561 cggccgccgg cctgatcgct gctgctgccg ccgcggatgc cgccgccccg cagcttggcg
     4621 gcgccggttc gccatgagca cgtcctcgga ccgctggcag caggcgcgca acatactcgc
     4681 catccgcctc gattacctcg gcgacgtcct gatgacgacg ccggcactgc gcgcgctgcg
     4741 caatcgcgcc ggcgggcgca tcacgctgct cacctcgcca ggcggtgcag cggtcgctcc
     4801 ctgcattcct gaaatcgacg acaccatcgt ctacgcagcg ccgtggctca agcacagcga
     4861 ggcgcccgat gccgcgcgcg atctggccat gatcgagacg ctgcgcgcgc gccgcttcga
     4921 tgcggcggtg atcttcacgg tctacagcca gaatccgctg cccgccgcgc tgctttgcca
     4981 cctcgccggc attccgcttc ggctcgcaca ttgccgcgag aacccgtacc agttgctgag
     5041 cgactgggtc gccgagacag agccgcaaca gcaggtgcgg catgaagtgc gccgccagct
     5101 cgacctcgtt gcaacggtcg gcgcgcgctg cgaggacgag cgcctgtcgt tttgcgtgcc
     5161 ggacaccgca caagaggcgg tgcgcgccca tcttgcccgc ctgcatgtgc gcaaacgatt
     5221 catcgtgctg catccgggcg cgagcgcggc atcgcgccgt tacccagcag cctcgttccg
     5281 ccgcgcggcc gagctgctct cccggaattt ccgccatccg ctcgttttta caggcagtac
     5341 cgccgaagcg ccgctgatcg acgagatccg ggccggcctg cccgacgcgc attcgctggc
     5401 gggcagactc tcgcttgccg agctcggcgc gctgctgcgg gaggccgccc tggtcgtcac
     5461 caacaacacc ggcccggccc accttgcagc cgccatcggc acgccagtcg tggacctgta
     5521 cgcgctcacc aatccgcagc acacgccatg gcaggtgccg gcgcgcgtgc tgttccagga
     5581 cgtttcctgc cgctactgct atcgcagcgc ctgcccgatg ggtcatcaag cctgccttgc
     5641 aagcgtggcg cccgagcgcg tgctcgaagc cgcggccgaa ttgctcggcg gcgcgcgtga
     5701 cagcagaccg gccttgccgc cgctctgacg tacggtagct tttacacgcc cggacacgca
     5761 atttctcgtg tgccgcattg ccggtgttgc ttgcgggccg gattttttcg tgctgcgtca
     5821 atgagttgcc agcaccgagg gcgtggcatg gagcttgcga agactgcagc accgagccac
     5881 caggagaacg aaatgccaag aagtgaagcg acatccaaaa ccaacaaaaa gacttcgccg
     5941 cgagcagccg gaaaagctgc ctcacaggat gcattgcgcc tgctagcgga ggatcacaag
     6001 aaggtaaaga agatgttcga cgagttcgag aagatcaagg acaaggagga catcgacgat
     6061 gagcaaaagc aactgctggt ggaaaccgct tgtgccgagc tgacgatcca cgcgcagctc
     6121 gaggaggagc tgttctaccc ggccgcgcgc gacgcgatcg acgacatgga cctgctcgac
     6181 gaagccgagg tcgagcatgc ttcagccaag cagctgatct ccgagctggc gacgatgcag
     6241 ccgggcgatg cgctgtacaa cgcgaagttc aaggtgctgg gcgagtacgt gaagcaccac
     6301 atcgaagagg aagaaaagga gcttttccct ctcatcaaga aatcggaact cgatcttgcg
     6361 gaactcgccg aggaaatgca ggaacgcagg atggaactgc gcgaagaact cgggtttacc
     6421 gccgtggaaa cggatgagga tgccgaggcg gccccgaagg cccgaaagaa tgtccactga
     6481 aatcggcaac ttggaagaaa ggcagctcgt gaccctgcaa caagaaccgg ttcctgccgc
     6541 cagcagcctg acccacgcgg tcgacatcct cgcgtttgtc gaggcgcagg aaattccgcc
     6601 ggattatttt gaaacgcctt acttcctcgc acccgagccg ggcggagaga aggactacgc
     6661 actattgcgc gaaaccctgc gccgcacgcg caagatcggc attgcctacg tggtgatcca
     6721 ggcacgccag cacctggcgg cgctgatccc gcatggccag tcgctggtgc tgaacacgct
     6781 gcgctggaac accgaggacc gcgagcgcgc gcgcacggca cgtgaagccg tggcggtggc
     6841 ggaccctgca cgtgcgccga tcgagcgcgg caccacgccg ccgctgcagc gcatgcgcgt
     6901 gaccggttgc gaggatttcg atccggacga ccttgaagaa ttgctggaag aaagcctgtt
     6961 cgactgcgac gacgtgatcg ccagcctgat gcgcccgcgg ctgcattaca gcgtgccgcc
     7021 ggcggggcgc ggaacgactc tgcgccagcc gctcggcgcc gcacgtccgc gccgttttgc
     7081 tcggcgcgcc tgatctcctc tccgatcttg ccatgttcgg gtttgcggag cactccggtg
     7141 ctccgctttt ttatgtccgc cagtcgggtc cgcgcggtgt cctgccgcgc aatcacgcct
     7201 tgatcacgct gatctgcccg tcggcctcca tgtagaccac ctttacctgg tcgagtgatt
     7261 ccacgccgga ctggcgcagc ttgctccaca gttcgtcttc cgagatgaac tcgcggcgca
     7321 tgttgcgcgg gagcaggcga ccccgctcgg ccagcttgat cggcttgggc tccgcgaagc
     7381 ggcgcacgaa tgcaaagcgg aacgccagcc agtccagcag cacgttccag ccgatcagcg
     7441 tcgccaccag cgccatgcca tccgccaggg acgtcgagtc gccggccatg ccgttctgcg
     7501 cggcatcagc gacgatgacc agcaccagca ggtccgcgat gccgaccgcg ccgacgtcgc
     7561 gccggatcac gaagcgaaac agcatgaaca ggaagcagta catgatcgac ccgcgcgcaa
     7621 tgatctccgt cagcggcacg gtcaacgaaa acagatcgcc ccagtcgggc atggcgagaa
     7681 gagcactcat ggatatcact cgaaacggtc aacgacgtgc ggcacgcgtg cgcactggcg
     7741 gcgcgcggcg cgaggcgggg gatgcagatc ctcagcgggc gcgctcggga ggcggcgcgc
     7801 cgccacgtgc ggctccgctg ccctgcccgg tgccggtcgt cgcagcaggt gcggagaggg
     7861 gatcgttgtg caggccgacg gcgtggcccg tccggcgtgc ccagtagccg tccacggtag
     7921 cgcgccagtg cgggtcgccg agatccggcc agcgcgccat gtcgaagccg ggcgcctcgc
     7981 gtatggtggg ccggtcggcg ttgagcacgt agg
//
LOCUS       DATLGF010000123         3401 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_84194,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000123 DATLGF010000000
VERSION     DATLGF010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84194"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VM406_03715"
     CDS             <1..416
                     /locus_tag="VM406_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HVL75098.1"
                     /translation="FGSVDLAVDLGIDCDPDGSEDELLHFRSHVVLASRVGGLAAPVD
                     GVSTAIDDVARLRADAERARRLGFGAKLCIHPKQVAAVQATFTPGAERVAWARRVCAA
                     FATAGGSAVAVDGQMIDKPLYERARAILSSAAGTR"
     gene            449..1234
                     /locus_tag="VM406_03720"
     CDS             449..1234
                     /locus_tag="VM406_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HVL75099.1"
                     /translation="MARKTKPIRSDIAWSTPDRIEVHGKSLPDELIGHINLGDMAFLQ
                     IVGRMPTPQESKLFNAVAVTLVEHGITPSALAARLTYAGAPESLQAAVAAGLCGLGSV
                     FVGSTEGTARMLYEALPPGARGADLEKIAREVVAGFRARGAIVPGLGHPLHKPVDPRA
                     PRLFQIAAETGFSGDYIKLEQLISAEAERLSGKMLPVNATGAIGAICCELGLPWKIVR
                     GIGVLARAIGLVGHILEESNNPMAVEIWQRVEDEATAHIRPGA"
     gene            1242..2252
                     /gene="bioB"
                     /locus_tag="VM406_03725"
     CDS             1242..2252
                     /gene="bioB"
                     /locus_tag="VM406_03725"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HVL75100.1"
                     /translation="MTAMETKTTLTPVQVVRRTIAAPAAADARWPLADVAALFELPFN
                     DLIFRAQQVHREHFDANEVQLSTLLSIKTGGCEEDCGYCPQSVHYDTGIEASKLLEPA
                     AVAAAARAAREQGASRFCMGAAWRAPKDRDIQKVVDLVRVVKAEGLEACCTLGMLTDT
                     QAQSLKHAGLDYYNHNVDTAPEAYGRIISTRTYDDRLQTLQRVREAGINVCCGGIVGM
                     GESRHERAGLVAQIANLDPYPESVPINQLVQVEGTPLHGTDVLDPFEFVRTIAVARIT
                     MPRARVRLSAGRQQMGEAVQALCFLAGANSIFYGDKLLTTGNPDWAADQRLLGSLGIT
                     AR"
     gene            2276..2659
                     /gene="panD"
                     /locus_tag="VM406_03730"
     CDS             2276..2659
                     /gene="panD"
                     /locus_tag="VM406_03730"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HVL75101.1"
                     /translation="MYRQMLKSKIHRATVTACELHYEGSCAIDVDLLDAADLRENEQV
                     HIWNIANGERFVTYAIRAQRGSGIISLNGSAARRAALGDLIIIAAFGWVAEAELAKHG
                     PKLVFLDERNRIRECRSTIPVKGAA"
     gene            2656..>3401
                     /gene="panB"
                     /locus_tag="VM406_03735"
     CDS             2656..>3401
                     /gene="panB"
                     /locus_tag="VM406_03735"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HVL75102.1"
                     /translation="MNIAGAIQGERKPVTLHRLRRMLADGEKIAMLTAYDASFARLAD
                     NAGMDCLLVGDSLGMVVQGRTSTVPVTLNEVAYHLRCVAAGNRNAWLLGDLPFGTYEA
                     GPNQALHSCAVLLQAGAQMVKLEGGWTVETVRFLVERGIPVCAHLGLTPQSANSRGGY
                     RVQGRDEASAQVLERQARELAGAGAAMLVLELVPAALAQRVQEQNPALVVIGIGAGAG
                     VAGQVLVLHDMLGVTQGKLPPSAMKLRTKRG"
BASE COUNT          574 a         1128 c         1167 g          532 t
ORIGIN      
        1 cgttcggcag cgtcgacctg gcggtcgacc tcggcatcga ctgcgaccct gacggcagcg
       61 aagacgagtt gctgcacttc cgctcgcacg tggtgctggc ttcgcgcgtg ggaggcttgg
      121 ctgcgcctgt cgacggcgtc agcaccgcca tcgacgatgt ggcgcggctg cgcgccgacg
      181 ccgagcgcgc gcggcgcctg ggcttcggcg ccaagctatg catccacccc aagcaggtgg
      241 ctgccgtgca agccaccttc accccgggcg ccgaacgcgt cgcctgggcg cgccgcgttt
      301 gcgcggcctt cgcaaccgcc ggcggttccg ccgtggcggt cgacggccag atgatcgaca
      361 agccgctcta cgagcgcgcg cgcgcgatcc tgagcagtgc agctggcacg agatgaactc
      421 cctaaagcag aacgaggaaa ggaacccaat ggcccgcaag accaaaccca tccgatcgga
      481 catcgcctgg agcacgcccg accgcatcga ggtccacggc aagagccttc ccgacgaatt
      541 gatcggccat atcaacctcg gcgacatggc ctttctgcag atcgtggggc gcatgccgac
      601 accgcaggag tcgaagctct tcaacgccgt ggcggtgacg ctcgtcgaac atggcatcac
      661 gcccagcgcg cttgcagcgc ggctgaccta cgcaggcgct cccgaatcgc tgcaggccgc
      721 cgtggcggcc gggctgtgcg ggctggggtc ggtgttcgtg ggctccaccg aaggcaccgc
      781 gagaatgctc tacgaggcgt tgccgccggg cgcgcgaggt gccgatctgg agaagatcgc
      841 gcgcgaagtc gtggccggct tccgtgcgcg gggcgccatc gtgccaggcc tggggcatcc
      901 cctgcacaag ccggtggatc cgcgggcccc gcggctgttt cagatcgctg cggaaaccgg
      961 gttttccggc gactacatca agctcgagca actgatcagc gcggaggccg agaggctgtc
     1021 cgggaagatg ctgccggtca acgctaccgg agcgatcggt gccatctgct gcgaactggg
     1081 cctgccctgg aagatcgtgc gcgggatcgg cgtgctcgcg cgagccatcg ggctggttgg
     1141 gcacattctg gaagaaagca acaatccgat ggcggtggag atctggcagc gtgtcgaaga
     1201 tgaggccacc gcgcatatcc gccccggcgc ttgaccctgg cgtgaccgcc atggaaacca
     1261 agacgaccct cacgcctgtc caggtggtgc gccgcaccat tgccgcgccg gccgcagcgg
     1321 atgcccgctg gcccctggcc gatgtggccg cgctgttcga gctgcccttc aatgacctga
     1381 tcttccgcgc ccaacaagtc caccgggagc acttcgacgc caacgaggtt cagctgtcaa
     1441 cgctgctgtc catcaagact ggcggctgcg aggaagactg cggctactgc ccgcagtcgg
     1501 tgcactacga cacggggatc gaggccagca aactcctgga gcccgcggcg gtggccgcag
     1561 ccgcgcgtgc tgcgcgcgaa cagggtgcgt cgcgcttctg catgggtgcg gcctggcgcg
     1621 cgcccaagga ccgcgacatc caaaaggtgg tcgatttggt gcgcgtggtc aaggccgaag
     1681 gcctggaagc ctgctgtacg ttgggcatgc tgaccgacac gcaggcgcag tccctgaagc
     1741 atgccggcct cgactactac aaccacaacg tcgacaccgc gcccgaggct tatgggcgca
     1801 tcatctcgac gcgcacctat gacgaccggc tgcagacgct gcagcgcgtg cgggaggccg
     1861 gcatcaatgt ctgctgcggt ggcatcgtcg gcatgggcga gtcgcgccac gagcgcgccg
     1921 ggctggtggc gcagatcgcc aacctcgatc cgtatccgga gtcggtaccc atcaaccagc
     1981 tggtgcaggt ggaaggcacg ccgctgcacg gcaccgacgt gctcgatcca ttcgagttcg
     2041 tgcgcaccat cgccgtggcg cgtatcacca tgcccagggc ccgcgtgcgc ctgtcggccg
     2101 gtcgccagca gatgggcgag gcggtgcagg cgctgtgctt cctggccggc gccaattcca
     2161 tcttctacgg cgacaagctg ctgaccacag gcaacccgga ctgggcagcc gatcagcgcc
     2221 tgctcggctc gctgggaatc actgcgcgct gaacgcggcg ccatgagaaa tcatcatgta
     2281 ccgccagatg ctgaaatcga agatccaccg cgcgacggtg accgcctgcg aactgcacta
     2341 cgagggctca tgtgccatcg acgtcgactt gttggacgcc gctgacctgc gcgagaacga
     2401 gcaggtgcac atctggaaca tcgccaacgg cgagcgtttc gtcacctatg ccatccgggc
     2461 gcagcgcggc agcggcatca tctcgctgaa cggctccgcg gctcgccgcg cagccctggg
     2521 cgacctgatc atcatcgcgg ccttcggctg ggtggccgaa gccgagctgg cgaagcacgg
     2581 cccgaagctg gtgttcttgg acgagcgcaa tcgcatccgt gagtgccgtt ccaccatccc
     2641 ggtgaagggc gcggcatgaa catcgcaggc gctatccagg gcgagcgcaa gcctgtcacc
     2701 ctgcaccggc tgcgtcgcat gcttgccgac ggcgagaaga tcgccatgct gacggcttac
     2761 gacgccagct tcgcccgcct agcggacaac gcgggcatgg actgtctgct ggtgggcgac
     2821 tcgctgggca tggtggtgca ggggcgcacg agcaccgtgc cggtgacgct gaacgaagtg
     2881 gcctatcacc tgcgatgcgt cgctgcaggc aaccgcaatg cctggttgct gggcgatctg
     2941 cccttcggca cgtatgaagc tggccctaat caggcgctgc actcctgcgc cgtgctgttg
     3001 caggccggcg cgcagatggt caagctggaa ggcggttgga cggtggagac cgttcgcttc
     3061 ttggtcgagc gcggcatccc ggtctgcgcg cacctgggcc tgacgccgca gagcgcaaat
     3121 tctcggggag ggtatcgggt ccagggtcgc gacgaagcca gcgcccaggt gctggagcgt
     3181 caagcccgcg aactcgcggg ggcgggcgca gccatgctgg tcctggaact cgtgccggcc
     3241 gcgctggcac agcgggtgca ggagcagaat ccggcgctgg tcgtcatcgg gatcggtgcc
     3301 ggagcgggcg ttgccggcca ggtactggtg ctgcacgaca tgctgggcgt cacccaaggc
     3361 aagctgccgc cctcggccat gaagttgcgc acgaaacgcg g
//
LOCUS       DATLGF010000124         4294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_37591,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000124 DATLGF010000000
VERSION     DATLGF010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37591"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1264)
                     /gene="dapC"
                     /locus_tag="VM406_03740"
     CDS             complement(71..1264)
                     /gene="dapC"
                     /locus_tag="VM406_03740"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HVL75103.1"
                     /translation="MNPLLSKLQPYPFEKLRQLFAGVTPNPQFSPISLGIGEPKHPTP
                     EFIKQALIDNLGGLASYPSTAGTDALRGAISGWLARRYGIPAPDPATQILPVSGSREA
                     LFALAQTVVDPVPGALVMCPNPFYQIYEGAALLAGAQPYFVNAEPSRNFAPDYRGVPA
                     DVWARVQLLYVCSPGNPTGAVLGLEDWKELFDLSDRYGFVIASDECYSEIYFKGEAPL
                     GGLEAAHRLGRSGYPRLIAFSSLSKRSNVPGMRSGFVAGDASVIKQYLLYRTYHGSAM
                     SPSVQAASVAAWNDERHVEENREKYIAKFAQITPMLQEVLDVQLPDAAFYLWAKVPQM
                     SDTEYARRLYAEYNVTVLPGSYLAREANGVNPGANRIRMALVAETEECLEAARRIVAF
                     TRSLS"
     gene            1430..>4294
                     /gene="smc"
                     /locus_tag="VM406_03745"
     CDS             1430..>4294
                     /gene="smc"
                     /locus_tag="VM406_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HVL75104.1"
                     /translation="MRLTSIKLAGFKSFVDPTHFQVPGQLVGVVGPNGCGKSNIIDAV
                     RWVLGESKASELRGESMQDVIFNGSTNRKAAGRASVELVFDNSDGRAAGQWSQYAEIA
                     VKRVLTRDGTSTYYINNQAVRRRDIQDIFLGTGLGPRAYAIIGQGMISRIIEARPEEL
                     RIFLEEAAGVSKYKERRRETENRLHDTRENLTRVEDILRELSANLEKLEAQAAVAQRF
                     RELQADQEEKQKLLWLLRRNEARNEQERFFREIEKAQTDLEEQTAKLRHIETELEQMR
                     HAHYAAGDRMHTAQGHLYQTNSEIGSLEAQIKFVIESRSRLQTQINTLTAQRDQWQRQ
                     GQQFQDDLAEAEMHLEEHAMKVEQAQQTAHDQNERIPALEQAWREAQLKATESRAKIM
                     QVQQQIELESAHQRNASNILSNLAARRERLAQEKSGMTLPDSAHLSNLKMQLEEKQAA
                     LEEARMQLEEAQEQQPKLEEERRGAQQQLNAETAANAQLEARLSALRQLQENVQTQGK
                     VQPWLQKHELGELPRLWQKLDVAQGWETALEAVLRERTSALELSNLDWAKAFFNDAPP
                     AKLALYTPNATAAPASEAPAGLKTFLSLLQLNDPGVRALMQDWLHNVFVAEDAATAFA
                     ERARLPAGGVFLTRQGHLVARSSVRFYASDSEQDGMLARQHEIENLTKQLRAQQMLAD
                     EAKARAVRSEAALSQATQRLQELRQRVAALTQGVHALQIEVMKLSEVEERFNQRSRQI
                     EADLAEIAAHEAEQQQVRVESEAKFEQLDIELAELQERHEQGQTAYLAQEQQLNEARL
                     RLRELERAAQEAEFAEKAHRNRIEELKRSIATALEQSAQLFASMQQGRLELENLDDQA
                     AQAGLQALLEKRSEQERALADARHELDQLTQKLRHAEEARMQAERSLQPLRERIMELQ
                     LKEQAARLNQEQYAQQLAEAQADEAALSEKLGGEMKASWLQ"
BASE COUNT          874 a         1325 c         1469 g          626 t
ORIGIN      
        1 gtcttcccac gcctggtcga tgatttgctg aagatcctgg gacatgcttg gtcgagtgtg
       61 aggttgagat tcaggaaagg gaacgggtga aggcgacgat gcgacgcgcc gcctcgagac
      121 attcttcggt ttcggcgacg agggccatgc ggatgcggtt cgccccggga ttgacgccgt
      181 tcgcctcgcg cgcgagatag cttcccggca acaccgtcac attatattcg gcgtacaggc
      241 ggcgcgcgta ctcggtgtcg ctcatctgtg gcactttcgc ccacaggtag aaggcggcgt
      301 ccggaagttg cacgtcgagc acttcctgca gcatcggcgt gatctgcgcg aacttggcga
      361 tgtacttctc gcggttttcc tcgacatggc gttcgtcgtt ccacgcagcc acggaggcag
      421 cctggacgga cgggctcatc gcgctgccgt ggtaggtccg gtacagcagg tattgcttga
      481 tgacggatgc atcgccggcc acgaagccgg aacgcatgcc ggggacattg gagcgcttgg
      541 acaggctcga gaacgcgatg aggcgtggat agccgctgcg cccgagcctg tgcgccgctt
      601 ccagtccgcc gagcggcgcc tcgcccttga aatagatctc cgaatagcat tcgtcggaag
      661 cgatcacgaa tccgtagcgg tcggaaaggt cgaacaattc cttccagtcc tccagcccca
      721 gcacggcgcc ggtggggttg cccggtgaac atacgtaaag cagttgcacg cgcgcccaga
      781 catcggccgg cacaccgcgg taatccggcg cgaagttgcg cgagggttcg gcgttcacga
      841 agtacggctg tgcgccggcc agcagcgccg caccttcata gatctgatag aacggattcg
      901 ggcacatgac cagtgcgccg gggaccgggt cgaccaccgt ctgggccagc gcgaacaagg
      961 cctcgcgcga gccgctgacc ggcaggatct gggtggcggg gtcgggcgcc gggataccgt
     1021 agcggcgcgc gagccagccg gaaatcgcgc cgcgcagcgc gtcggtgccg gcggtgctcg
     1081 gatagctggc caggcccccc agattgtcga tcagcgcctg cttgatgaat tccggcgtcg
     1141 gatgcttcgg ctcgccgatt ccgaggctga tcgggctgaa ctgcgggttc ggcgtgaccc
     1201 cagcgaacag ttgcctcagt ttttcgaacg ggtagggttg cagcttcgac aagagcggat
     1261 tcacgggatg ggcagggggg aggtgagtgg aatggcaagt gccgcaaacg cggcattata
     1321 ccgtcgtgca tcgaggccgt ccgagtggca aagcggctgg ggcccgatgt tatgataggc
     1381 gcctttcaag cggggctcgc cccgtggcaa gcaaggaacg gttgacaacg tgcgcctaac
     1441 ttcgatcaaa ctcgcgggat tcaagtcgtt cgtggacccc acccacttcc aggtgccggg
     1501 ccagctggtc ggcgtagtgg gaccgaacgg ctgcggcaag tccaacatca tcgatgcggt
     1561 gcgctgggtg ctcggcgaat ccaaggcgtc cgagctgcgc ggcgaatcga tgcaggacgt
     1621 gatcttcaac ggatcgacca accgcaaggc ggccggacgc gcatccgttg aactggtgtt
     1681 cgacaacagc gacgggcgtg ccgccggcca gtggagccag tatgccgaaa tcgcggtcaa
     1741 gcgcgtgctc acgcgcgacg gcacctcgac ctattacatc aacaaccagg cggtgcgccg
     1801 ccgcgacatc caggacatct tcctcggcac cggcctcggt ccgcgcgcct acgccatcat
     1861 cggccagggg atgatctcgc gcatcatcga agcgcgcccg gaggagctgc gcatcttcct
     1921 ggaagaggcc gctggcgtat cgaaatacaa ggaacgccgg cgcgaaaccg agaaccgcct
     1981 gcacgatacc cgcgagaacc tgacgcgggt ggaagacatc ctgcgcgaac tgtccgctaa
     2041 cctggagaag ctggaagccc aggcggcggt cgcgcagcgc ttccgtgagc tgcaggccga
     2101 ccaggaggag aagcagaagc tgctttggct gctgcgccgg aacgaagcca ggaacgagca
     2161 ggaacgattc ttccgagaga tagagaaggc gcagaccgat ctcgaggaac agaccgcgaa
     2221 gctgcgccac atcgaaaccg agctcgagca gatgcggcac gcacactacg cggccggcga
     2281 ccgcatgcac actgcccagg gccacctgta ccagaccaat tccgagatcg gcagcctgga
     2341 agcgcagatc aagttcgtga tcgagtcgcg ctcccgtctg cagacacaga tcaacacact
     2401 caccgcgcag cgcgaccagt ggcagcgcca gggccagcaa ttccaggatg acctcgccga
     2461 agccgagatg cacctggaag aacacgcaat gaaggtggag caggcgcagc agaccgcgca
     2521 cgatcagaac gagcgcatcc cggcactgga acaggcctgg cgggaagcgc agctcaaggc
     2581 caccgagtcg cgcgcgaaga tcatgcaggt ccagcagcag atcgagctgg agtccgcaca
     2641 ccagcgcaac gcgtcgaaca tcctctccaa tctggccgcc cgccgcgagc gcctggcgca
     2701 ggaaaagagc ggcatgacgc tgccggacag cgcgcacctg tccaatctga agatgcagct
     2761 ggaagagaag caggcggctc tggaagaggc gcggatgcag ctggaggaag cgcaggagca
     2821 gcagccgaag ctggaagaag agcggcgcgg cgcccagcag cagctcaatg cggaaacggc
     2881 cgccaacgcc cagctggaag cgcgcctgag tgccctgagg cagctgcagg aaaatgtgca
     2941 gacccagggc aaggttcagc cctggctgca gaagcacgaa cttggcgagc tgccgcgcct
     3001 gtggcagaag ctcgatgtgg cccagggctg ggagacggcg ctggaagccg tgctgcgcga
     3061 gcgcacctct gcgctggaat tgtcgaacct cgactgggcc aaggcgtttt tcaacgatgc
     3121 gccgccggcg aagcttgccc tttacacccc caatgccaca gcggcgcccg catccgaggc
     3181 gccggccggc ctcaagacct tcctgagcct gttgcagctc aatgatcctg gcgtgcgcgc
     3241 gctgatgcag gactggctgc acaacgtgtt cgtcgcggaa gatgcggcga cggccttcgc
     3301 cgaacgtgcg aggctgccgg ccggtggcgt gttcctcacg cgccaggggc atctggttgc
     3361 tcgctcgagc gtgcgcttct atgcgtcgga ctccgagcag gacggtatgc ttgcgcgcca
     3421 gcacgagatc gagaatctga ccaagcagct gcgcgcacag caaatgcttg ccgacgaggc
     3481 caaggcgcgg gcggtgcgtt ccgaggccgc gctgagccaa gccacgcagc ggctgcagga
     3541 attgcgccag cgtgtagccg cgctgacgca gggcgtgcat gcgttgcaga tcgaggtcat
     3601 gaagctgtcc gaggtcgagg agcggttcaa ccagcgcagc cggcagatcg aggccgatct
     3661 ggccgagatc gccgcgcacg aagccgagca gcagcaggtc agggttgaat cggaagcgaa
     3721 attcgagcag ctcgacatcg aactggccga actgcaggaa cggcatgagc aggggcagac
     3781 cgcctacctg gcccaggagc agcagttgaa cgaggcgcgc ctgcgcctgc gcgagctcga
     3841 acgcgccgcc caggaggcgg aatttgcgga gaaggcgcac cgcaaccgca tcgaagagtt
     3901 gaagcgcagc attgccaccg cgctggaaca gagcgcgcaa cttttcgcga gcatgcagca
     3961 gggtcggctc gaactcgaaa acctggacga ccaggcggcc caggccggct tgcaggcgct
     4021 gctggaaaag cgcagtgagc aggagcgcgc gctggccgat gcccgccacg agctcgacca
     4081 gctgacccag aagctgcgcc atgccgagga agcccggatg caggcagagc gctcgctgca
     4141 gccgctgcgc gaacgcatca tggagctgca gctgaaggag caggccgcgc ggctgaatca
     4201 ggagcagtac gcgcagcagc tcgccgaggc gcaggcggac gaggcggcgc tctcggagaa
     4261 gctgggcggc gagatgaagg cgtcctggct gcag
//
LOCUS       DATLGF010000125         4936 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_161905, whole genome shotgun sequence.
ACCESSION   DATLGF010000125 DATLGF010000000
VERSION     DATLGF010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161905"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VM406_03750"
     CDS             complement(<1..668)
                     /locus_tag="VM406_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HVL75105.1"
                     /translation="MSYVINPPSVPAVPVAGSDQLFPVHRIYCVGRNYAEHAKEMGAT
                     GREAPFFFMKPGDAVLPVAEGAIGELPYPGQTADVHHEIELVVAIGKGGRDIPAAQAL
                     DHVWGYAIGLDMTRRDLQAEAKKHGRPWCTAKGFDHSAPIGPIHPAAALGDPAEAQIR
                     LEVNGAPRQNSVVGKMIWNIAETIEHLSKYFELQPGDLIFTGTPEGVAAVQRGDVLEG
                     SVSNL"
     gene            complement(798..1523)
                     /locus_tag="VM406_03755"
     CDS             complement(798..1523)
                     /locus_tag="VM406_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL75106.1"
                     /translation="MSQNVLKISGLKIAYGGIQAVKGIDLEVNEGELVTLIGANGAGK
                     TTTLKAITSTLPGCKVEGEIRYVGEPIQGADSFKLVKRHLAMVPEGRGVFTRMTILEN
                     LQMGAYIRNDKGGIEADIDKWFGVFPRLKERSSQLAGTLSGGEQQMLAMARALMSHPK
                     LLLLDEPSMGLSPIMVEKIFEVVRAVSAQGVTILLVEQNAKLALQAAHRGYVMDSGLI
                     TMTGDAKDMLHDPKVREAYLGEG"
     gene            complement(1523..2293)
                     /locus_tag="VM406_03760"
     CDS             complement(1523..2293)
                     /locus_tag="VM406_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL75107.1"
                     /translation="MSEQIILNIAGVNKRFGGLQALSEVSVKIMRGQIYGLIGPNGAG
                     KTTFFNVITGLYQPDSGSFELAGKPYSPSAPHEVAKAGIARTFQNIRLFGEMSALENV
                     MVGRHVRTKQGVLGAVLHHKAAREEEAGIRKRAMELLDFVGIAHFAHRTSRHLSYGDQ
                     RRLEIARALATDPQLLALDEPAAGMNATEKLALRELLVKIKDEGKTILLIEHDVKLVM
                     GLCDRITVLDYGKPIAEGLPAEVQKNPAVIEAYLGGAH"
     gene            complement(2305..3525)
                     /locus_tag="VM406_03765"
     CDS             complement(2305..3525)
                     /locus_tag="VM406_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL75108.1"
                     /translation="MSALFDVKHNPQKAYISFTILAIVLIAFPFVAAPFGNSWVRIAD
                     LALLYILLALGLNIVVGFAGLLDLGYIAFYAMGAYMTGLLASPQMAAVIESFLYNYPA
                     VGEFLVWLLGEEIRQNGIHLSVWVIVPLGAFLAAILGALLGAPCLKLRGDYLAIVTLG
                     FGEIIRIFLNNLNGPVNITNGPQGINMIEPIRVFGVNLAGEPGSRATVYIGNFSMPSV
                     NAYYFLFLFLVVLTVIICVRLQNSRLGRAWVAIREDEIAAKAMGINTRNVKLLAFAMG
                     ASFGGVAGAMFASFQGFVSPESFTLMESIAVLAMVVLGGIGHIPGVILGGVILASLPE
                     VLRHIVEPVQRTLFGQVLVDAEVMRQLLYGLAMVVIMLYRPAGLWPSPRHEDRMDANA
                     PKRHDEKEPAALAA"
     gene            complement(3549..4478)
                     /locus_tag="VM406_03770"
     CDS             complement(3549..4478)
                     /locus_tag="VM406_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVL75109.1"
                     /translation="MDILIQQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDVLM
                     IGAMVGIMILKVLQNVAPELPGVVKLAIAILGAIPVCAMVNVIIERVAYRRLRNAPRL
                     APLITAIGVSILLQTFAMLIWGRSPIPFPQVMPSEPFHVFGALISPTQVMLLVLAMIS
                     MALLVLLVEKTKMGRAMRATAENPRVAGLMGVDANKVIVATFAIGAALAAVAGVMWGA
                     NYATAQFAMGFIPGLKAFSAAVLGGIGNIYGAMVGGILLGLIEALGAGYIGDLTGGFL
                     GSHYQDIFAFIVLIIVLTLRPSGIMGERVADRA"
BASE COUNT          948 a         1569 c         1517 g          902 t
ORIGIN      
        1 agatttgaca ccgagccctc cagcacatcg ccgcgctgca ccgctgcgac gccttcgggc
       61 gtgccggtga agatcaggtc gccgggctgc agctcgaaat acttcgacag atgctcgatg
      121 gtctcggcaa tgttccagat cattttcccc acgacgctgt tctggcgcgg cgcgccgttt
      181 acctcgagac ggatctgcgc ctccgccgga tcgccaagcg ctgcagcagg atgaatcggc
      241 ccgatcggtg cggaatggtc gaaccctttc gccgtgcacc acgggcgtcc atgcttcttg
      301 gcctcggcct gcaggtcgcg ccgcgtcatg tcgaggccga tcgcatatcc ccatacatga
      361 tcaagcgcct gcgcggccgg aatgtcgcgt ccgcccttgc cgatcgcgac taccagctcg
      421 atctcgtgat gcacgtcggc agtctggccc ggataaggca gttccccgat cgctccttcc
      481 gcaaccggca gcaccgcgtc acccggtttc atgaagaaga acggcgcctc gcggccggtc
      541 gcgcccattt ccttcgcgtg ctcggcatag ttgcgtccga cgcagtagat gcggtgaacc
      601 ggaaagagct gatcgctgcc tgcgacaggc acggcgggaa cggagggcgg gttgatcacg
      661 tacgacatga atggcctcga agggatgcag tgaaggaggc gtcgattatg ccaccgggca
      721 aagacgcatg ctttgccccc ttgaaaagca gagccgcccc gtcgcagcgc gaacggggcg
      781 gcactgtcgg tagccgatca gccctcgccg agataggcct cgcgcacctt cggatcgtgc
      841 agcatgtcct tggcgtcgcc ggtcatcgtg atcagaccgg agtccatcac gtagccgcgg
      901 tgggcagcct gaagagccag cttcgcgttc tgttcgacca gcagaattgt gaccccctgg
      961 gcggataccg cgcgcacaac ctcgaaaatc ttctcgacca tgatcggcga cagacccatt
     1021 gaaggctcgt ccagcagcag aagcttcgga tggctcatca gcgcgcgtgc catcgccagc
     1081 atctgctgct cgccgccgga cagcgtgccg gccagttgcg aggaacgctc cttcaggcgc
     1141 gggaacacgc cgaaccactt gtcgatgtca gcctcgatgc cacccttgtc gttgcggatg
     1201 taggcgccca tctggagatt ctcaaggatg gtcatgcggg taaacacgcc gcgaccttcc
     1261 ggcaccatcg ccagatgacg cttgaccagc ttgaacgagt ccgctccctg gatcggctcg
     1321 cccacatagc ggatctcgcc ttcgaccttg cagcccggaa gcgtactggt aattgccttg
     1381 agcgtcgtgg tcttgcctgc gccgttggcg ccgatcaggg tcaccagttc gccctcgttg
     1441 acttcgaggt cgatgccctt gaccgcctgg atgccgccgt aagcgatctt caggccgctg
     1501 atcttcaata cgttctgact cattagtggg cacctcccag atatgcctcg atcaccgccg
     1561 ggttcttctg cacctcggcc ggcaggcctt ctgcaatcgg cttgccgtag tccagcacgg
     1621 tgatgcggtc gcacagtccc atcaccagct tcacgtcgtg ttcgatcaat aggatggtct
     1681 tgccttcgtc cttgatcttg accagcagct cgcgcagcgc gagcttctcg gtcgcgttca
     1741 tgccggcggc cggctcgtcc agcgcaagca gctgcggatc ggtcgccagc gcgcgcgcaa
     1801 tctccaggcg gcgctggtcg ccataggaca gatgccggga ggtgcggtgg gcgaagtgcg
     1861 cgatgccgac aaagtccagc agctccatcg cgcgcttgcg gataccggct tcttcctcgc
     1921 gcgcggcctt gtggtgcagc accgcgccga gcacgccctg cttggtgcgc acgtgccggc
     1981 ccaccatcac gttctccagc gccgacatct cgccgaacag ccggatgttc tggaaggtac
     2041 gcgcgatccc ggccttggcc acttcatgcg gggccgacgg cgagtacggc ttgccggcca
     2101 gttcgaagct gcccgaatcg ggttggtaca ggcccgtgat gacgttgaag aaggtggtct
     2161 tgccggcgcc attggggccg atcagcccgt agatctggcc gcgcatgatc ttgacgctta
     2221 cctcggagag cgcctgcaga ccgccgaagc gcttgttgac gcctgcgata ttgagaatga
     2281 tttgttcgct cattgcagtg atccttatgc agcgagggcc gcgggttcct tttcgtcgtg
     2341 acgcttgggc gcattcgcgt ccatgcggtc ctcatgcctg ggcgagggcc acaggccggc
     2401 cggtcggtac agcatgatca cgaccatcgc caggccgtag agcagctgac gcatcacttc
     2461 ggcgtcgacc agcacctggc cgaacaaggt gcgctgcacc ggttcgacga tatggcgcaa
     2521 cacttccggc agcgaagcga ggatcacccc gcccaggatc acgcccggga tgtggccgat
     2581 tcctcccagc acgaccatcg cgagcacggc gatcgattcc atcagcgtga acgattccgg
     2641 cgagacgaag ccttggaacg acgcgaacat cgcaccggcc acgccaccga acgatgcccc
     2701 cattgcgaag gccagcagct tgacgttgcg ggtattgatg cccatcgcct tggcggcgat
     2761 ctcatcttcg cgaatcgcca cccatgcacg gcccaggcgc gagttctgca ggcgcacgca
     2821 gatgatcacc gtgagcacga cgaggaacag gaacaggaag tagtacgcat tcaccgaagg
     2881 catcgaaaaa ttgccgatgt agacggtcgc gcgcgagccc ggctcgccgg cgaggttcac
     2941 cccgaacacg cggatcggct cgatcatgtt gatgccctgc gggccgttgg tgatgttgac
     3001 cgggccgttc aggttgttca ggaagatgcg gatgatttcg ccaaagccca gcgtgacgat
     3061 ggcgaggtag tcgccgcgca gcttcaggca cggcgctccc agcagcgcac ccagtatcgc
     3121 cgcgagaaag gcaccgagcg gcacgatcac ccagaccgag aggtgtatcc cgttctggcg
     3181 gatttcctcg ccaagcaacc agaccaggaa ctcgccgacg gccgggtagt tgtagaggaa
     3241 ggactcgatc accgcggcca tctgcggaga agccagaaga ccggtcatgt aggcgcccat
     3301 cgcgtagaac gcgatgtaac cgaggtccag caggccggca aaaccgacca cgatgttcag
     3361 gccgagcgcc agcaggatgt acagcagcgc gaggtcggcg atgcggaccc aggagttacc
     3421 gaacggcgcg gccacgaacg ggaaggcgat cagcacgatc gcgaggatcg tgaagctgat
     3481 gtaggccttc tgcggattgt gtttgacgtc gaatagtgcg gacatggttc tctccttttc
     3541 ctgtcgcgtc aggcgcgatc cgcaacgcgt tcgcccatga tgccggacgg ccgcagcgta
     3601 agcacgatga tcagtacgat gaatgcaaag atgtcctggt aatggctgcc gaggaagccg
     3661 ccggtcaggt cgccgatgta gccggcgccg agcgcctcga tcaggccgag caggatgccg
     3721 ccgaccatcg cgccgtagat atttccgata ccgcccagca ccgctgcgga gaacgccttc
     3781 agcccgggga tgaagcccat tgcgaactgc gcggtcgcat agttggcgcc ccacatcacg
     3841 ccggccaccg cggccagcgc cgcaccgatc gcaaaggtcg cgacaatgac cttgttcgcg
     3901 tccacgccca tcaggcccgc cacgcgcggg ttttcggcgg tggcgcgcat tgcgcggccc
     3961 atcttggtct tctcgacgag cagcaccagg agcgccatcg aaatcatcgc cagcaccagc
     4021 agcatcacct gcgtcggtga gatcagcgcg ccgaacacgt ggaacggttc ggatggcatg
     4081 acttgcggga acggaatcgg gctgcggccc cagatcagca tcgcgaaggt ctgcagcagg
     4141 atggacacgc cgatggccgt gatcagtgga gccagtcgcg gcgcattgcg caggcggcgg
     4201 taggccacgc gttcgatgat cacgttgacc atcgcgcaca ccgggatcgc accgaggatc
     4261 gcgatcgcca gcttcaccac gccgggaagc tcgggtgcca cgttctgcag caccttcagg
     4321 atcatgatcc cgaccatcgc accaatcatc aacacgtcgc catgcgcgaa gttgatcagg
     4381 ttaagcacgc cgtacaccat cgtgtaaccg agcgcgatca gcgcatacat gctgcccagc
     4441 actaatccat tgatgatttg ttggataagg atatccatag ttgcctactc tctataaggt
     4501 cgtttaatgc agttgacgca tgtccgctgc cgccgatgcg tgtcggaggc atgcgtgcgc
     4561 cgtataacaa aagcggcacc gcccggggtg ccgcctgcaa tcaacgatgg ttacctggaa
     4621 agatttgccg caatgcgagg gatggcggtg aagggagtgt tgcgcatgag tcgtgtctcc
     4681 tggcgtgtct ctggtgaaaa ctttttgtaa tagtgagtga ggccttgtga gccgcaccgg
     4741 acgggcgcga ttatacccaa catgttccgc gatgaaacta tggcgtatac ccaacaccgg
     4801 caagacgcga acgattgcgc ccgccccgcg atatgcggta ggcgccttgc cgaccgtgcc
     4861 gcctctgcat gcctacgctt tgcagattgc aatcatagcc acgttcgaca agaacaatcg
     4921 ttggaaatag tttggc
//
LOCUS       DATLGF010000126         6508 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_21804,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000126 DATLGF010000000
VERSION     DATLGF010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21804"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2140)
                     /locus_tag="VM406_03775"
     CDS             complement(<1..2140)
                     /locus_tag="VM406_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HVL75110.1"
                     /translation="MQNLLHNLNPEQLAAVTLPAQSALILAGAGSGKTRVLTTRIAWL
                     IQTGQVSPAGVLAVTFTNKAAKEMLSRLAAMLPINTRGMWIGTFHGLCNRLLRAHYRD
                     AGLPQTFQILDSTDQLSAIKRLLKTLNVDDEKYPPRNLMYFINNAKEQGLRAHDVEAY
                     DDYNRRFVELYEAYDHQCQREGVVDFAELLLRTYELLMRNQPLREHYQARFKHILVDE
                     FQDTNDLQYKWLKLMAGSGNAVFAVGDDDQSIYAFRGANVGNMVAFEREFHVKNLIKL
                     EQNYRSHGHILDAANILISHNSRRLGKNLRTDAGHGEPVRVYEASSDIQEAQWVIEEA
                     KNLINEGAARSEIAILYRSNAQSRVIEHALFTAGIPYRVYGGQRFFERAEIKHAIAYL
                     QLMDNPHNDSAFLRVVNFPARGIGARSIEQLQDAARTHGLSLYAAVAHVGGKAGSTLG
                     SFVRLVESMRFEAQNLPLQEMVQLMLDMSGLVTHYQNEKEGADRIENLEQLVSAAMLF
                     VSEEGYGIDAPAFLGPRAQASAGTLLSGGDEVLDADAPLPTVMSALSAFLSHASLEAG
                     DNQAQAGQDALQLMTVHSAKGLEFDAVFITGLEEGLFPHDNSAQEEGGLEEERRLMYV
                     AITRARKRLYMSLSQTRMLHGQTRYNMKSRFLDELPEGSLKWLSPRVQPHWFGSRKSA
                     WDDAVDVEANTIAQTFARKDASGWRAGQTVS"
     gene            complement(2190..2669)
                     /locus_tag="VM406_03780"
     CDS             complement(2190..2669)
                     /locus_tag="VM406_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75111.1"
                     /translation="MSNLGSAKAAIKAELQHAREGVAYYQSLVTALEEALNRLDSVGP
                     ETAKAPGRKGAATAVTAVKATRGRRGRKPRIETGAAQEGELPGTRKEFWTDLVDAQPK
                     SAVEVVNAAVEKLDFTPTREQVKKLAQRATYALNTMVKSGEISDSGSGRARRFFRAQ"
     gene            complement(2787..3395)
                     /gene="thiE"
                     /locus_tag="VM406_03785"
     CDS             complement(2787..3395)
                     /gene="thiE"
                     /locus_tag="VM406_03785"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HVL75112.1"
                     /translation="MKGLYLVTPDWDDTERMIAATEAGLRGGAILVQYRHKTAQPTLR
                     KQQAEALLALCRRYEKPFIVNDYVELGMEIGADGVHVGGTDMDVSKVRALLGPDRIVG
                     ASCYGSLELARRAHAAGASYVAFGGFYPSRVKKYEVTTSPTIVQESKAELTLPVVVIG
                     GMTSENARPLVKHGTDMVAVISSVYMSEDPEKAARAFSELFV"
     gene            complement(3392..4183)
                     /locus_tag="VM406_03790"
     CDS             complement(3392..4183)
                     /locus_tag="VM406_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HVL75113.1"
                     /translation="MTRRCVLVFAGSDPSGGAGLQADLQAIAAQGAHALTVVTAVTVQ
                     DNDRVFSVNAVAAELVTRQAQALIDKIDVAAVKIGIPGSLPNAEAIAAIVAHLHHRNP
                     GLPVVLDTVLASGKGDALSSGDPIDGVRSLLPHATLVMPNLPEAARLCPGQSEIDVQA
                     QALLRACPNVLIKGGHGGGDEVVNTWFARDVRHSWRWPRLAGQFHGTGCTLASAVAGL
                     LAQGLPMRAALEAAQSYVHQTLVAAYAIAPGQRIPQRSPTSREIQ"
     gene            complement(4180..4977)
                     /locus_tag="VM406_03795"
     CDS             complement(4180..4977)
                     /locus_tag="VM406_03795"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HVL75114.1"
                     /translation="MSDQSTPDQGLTIAGKTYRSRLLVGSGKYKDLEQTRLATEASDA
                     EIITVAIRRVNIGQDPNAPNLLDVVPPSHYTILPNTAGCYNAKDAVYTLQLARELLNG
                     HKLVKLEVLGDERTLFPNMPETLKAAETLVNDGFDVMVYCSDDPIQAKMLEEIGCVAV
                     MPLASLIGSGMGILNPWNLSLIIEQAKVPVLVDAGVGTASDAAIAMELGCDGVLMNTA
                     IAGAKDPVRMARAMKLAVEAGREAYLAGRIPKRFAASPSSPMEGRAT"
     gene            complement(5049..5249)
                     /gene="thiS"
                     /locus_tag="VM406_03800"
     CDS             complement(5049..5249)
                     /gene="thiS"
                     /locus_tag="VM406_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HVL75115.1"
                     /translation="MEIELNGAPHPVAENLNVQDLIASLELTGKSLAVAINREVVPRQ
                     RWAERVLQAGDKVDVVKAIGGG"
     gene            complement(5252..>6508)
                     /gene="thiC"
                     /locus_tag="VM406_03805"
     CDS             complement(5252..>6508)
                     /gene="thiC"
                     /locus_tag="VM406_03805"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HVL75116.1"
                     /translation="NINHPETEPMAIGRNFLVKINANIGNSAVTSSIGEEVEKMTWAI
                     RWGADTVMDLSTGRHIHETREWIIRNSPVPIGTVPIYQALEKVHGKAEELTWEIFRDT
                     LIEQAEQGVDYFTIHAGVRLQYVPLTAKRMTGIVSRGGSIMAKWCLAHHKESFLYEHF
                     EEICEIMKAYDVSFSLGDGLRPGSIYDANDEAQLGELKTLGELTQIAWKHDVQVMIEG
                     PGHVPMHLIKENMDIQLEQCKEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWYGTAM
                     LCYVTPKEHLGLPNKDDVKEGIITYKLAAHAADLAKGHPGAQVRDNALSKARFEFRWE
                     DQFNLGLDPDKAREFHDETLPKDSAKVAHFCSMCGPHFCSMKITQDVRDFAASQGISE
                     DDALKKGMEVKAVEFVKSGAELYRKQ"
BASE COUNT         1144 a         2068 c         1942 g         1354 t
ORIGIN      
        1 gtgaaacggt ctggccggcg cgccatcccg atgcgtcctt gcgcgcgaaa gtctgggcga
       61 tcgtgttggc ttcaacatcg acggcgtcgt cccatgccga cttgcgtgac ccgaaccagt
      121 gcggctgtac gcgcggcgag agccacttca gcgagccttc aggcagctcg tcgaggaaac
      181 gcgacttcat gttgtagcgg gtctggccgt gcagcatgcg tgtttgcgac aagctcatgt
      241 agaggcgctt gcgcgcgcgc gtgatcgcca cgtacatcag ccggcgctct tcctccagcc
      301 cgccctcttc ctgcgcgctg ttatcgtgcg ggaacagtcc ttcttccagg ccggtgatga
      361 ataccgcatc gaactccagc cccttggccg aatgcacagt catcaattgc agcgcatcct
      421 gcccggcctg ggcctggttg tcgccggctt ccagcgacgc gtgcgagagg aaggcggaca
      481 gcgccgacat cacggtcggc agcggcgcgt cggcgtcgag cacttcatcg ccgccggaaa
      541 gcagcgtccc cgccgaggcc tgcgcgcgcg gaccgaggaa ggccggcgca tcgatcccgt
      601 agccctcctc ggaaacgaac agcatcgcgg cgctgaccag ctgctcgagg ttctcgatac
      661 ggtccgcgcc ttctttctca ttctgatagt gcgtgaccag accgctcata tccagcatca
      721 gctgcaccat ctcctgcagc ggcaggttct gcgcctcgaa gcgcatgctc tcgaccagcc
      781 tgacaaaact gccgagcgta ctgccggcct tgccgccgac gtgggctacc gccgcataga
      841 gcgacaggcc atgcgtgcgc gccgcatcct gcaattgttc tatcgagcgg gcgccgatgc
      901 cgcgcgccgg gaaattcacc acgcgcagga acgccgaatc gttgtgcggg ttgtccatca
      961 attgcaaata ggcgatcgcg tgcttgattt cagcgcgctc gaagaagcgc tgcccgccgt
     1021 agacgcgata cggaatgccc gcggtgaaca gcgcatgttc gatcacccgc gactgggcat
     1081 tcgaacgata gaggatcgcg atttcgctgc gcgcggcccc ttcattgatc aggttcttcg
     1141 cctcctcgat cacccactgc gcttcctgga tgtcgctgct ggcctcatac acgcgcaccg
     1201 gttctccgtg cccggcatcg gtgcgcaggt tcttgcccag gcgcctgctg ttatgcgaaa
     1261 tgaggatatt ggcggcatcg agaatatggc cgtgcgagcg gtagttctgc tcgagcttga
     1321 tcaggttctt gacatggaat tcacgctcga aggcgaccat gttgccgaca ttggcgccgc
     1381 gaaacgcgta gatgctctga tcgtcgtcgc ccactgcaaa gacggcgttg ccgctaccgg
     1441 ccatcagctt cagccatttg tattgcaggt cgttggtatc ctgaaattcg tcgacgagga
     1501 tgtgtttgaa acgcgcctgg taatgttcac gcaacggctg gttgcgcatc agcagttcat
     1561 acgtccgcaa caacagctcg gcgaaatcga cgacgccttc gcgctggcac tgatggtcat
     1621 acgcctcgta caactcgacg aatcgccggt tgtaatcatc gtaggcctcc acatcgtgtg
     1681 cgcgcagccc ctgctccttc gcattgttta tgaaatacat caggttgcga ggcgggtact
     1741 tttcatcgtc cacattcaag gtcttcaaca gacgcttgat agctgaaagc tggtcggtgg
     1801 aatcgagaat ctggaatgtc tgcggaagcc cggcatcgcg ataatgcgcg cgcagaagac
     1861 ggttgcacaa gccatgaaag gtgccgatcc acatcccgcg tgtattgatc ggcaacattg
     1921 ccgcaaggcg cgacagcatt tccttcgcgg ccttattggt aaacgtgact gcaagcacgc
     1981 cggccggcga cacctggccg gtctggatca gccatgcgat gcgcgtagtg agcacgcgcg
     2041 tcttgcctga gcccgccccg gccaggatca gggccgattg tgcgggaagg gtgacggcgg
     2101 cgagttgttc gggattgaga ttgtggagga gattctgcat ccggcgatta tagtcctcca
     2161 ggggaggcgc gccggatgca ggtgacgcgt tattgcgccc ggaagaagcg gcgggcgcgg
     2221 ccgctgccgg aatcggagat ttctccgctc ttgaccatcg tgttcaacgc ataggtggca
     2281 cgctgcgcaa gcttcttcac ctgttcgcgc gtcggcgtga agtcgagttt ttcaacggcg
     2341 gcgttcacaa cttcgacagc ggacttcggc tgagcgtcga ccaggtcggt ccagaattcc
     2401 ttgcgggtgc cgggcagttc tccttcctgc gcggcgcccg tttcaatgcg cggcttgcgg
     2461 ccgcggcgcc cgcgcgtggc cttcacagcc gtcaccgccg tcgcggcgcc cttgcgtccc
     2521 ggtgcctttg cggtttcagg gccgacgctg tccagacggt ttaatgcttc ttcgagcgcc
     2581 gtcaccagcg actgataata agcgacgcct tcgcgggcgt gttgcagttc agccttgatc
     2641 gccgcttttg cagaaccgag attcgacatg gatattctcc tggtgttgtc gtttactgtt
     2701 gcaacttcga atttttttga ttctaccagt aagcgttaac acttgttgaa ttccagtttg
     2761 aagcgcatca atgatttccg aaatcttcaa acaaaaagtt cagaaaacgc acgcgccgct
     2821 ttctccgggt cttcagacat gtaaacactg ctgatgaccg cgaccatatc tgttccatgt
     2881 ttgacaagag ggcgcgcatt ctcgctggtc atgccgccga tgacgacgac gggcagtgtc
     2941 aattccgcct tggattcctg aacgatggtt ggagatgttg ttacttcata cttttttacg
     3001 cgcgaaggat aaaaaccgcc gaaggccacg taactcgccc ctgccgcatg cgcgcgacgc
     3061 gccagttcaa gactcccgta gcaggaggca cctacgatgc ggtccggtcc gagcaaggcg
     3121 cgcaccttcg agacgtccat gtccgttccg ccgacatgaa cgccgtcggc gccgatttcc
     3181 ataccgagtt cgacgtaatc attcacgatg aacggttttt catatcgacg gcacagtgcg
     3241 agcagggcct cggcctgctg tttgcgcaac gtgggctgcg ctgtcttgtg acggtattgc
     3301 acgagtatcg ccccaccccg caatcccgcc tctgtggcag caatcatgcg ttcggtgtcg
     3361 tcccagtcgg gcgtaacgag atataagcct ttcattgaat ttcccttgaa gttggactcc
     3421 gctgcggaat gcgctggcca ggcgcgatcg cataggcagc gacaagcgtt tgatggacat
     3481 atgactgcgc cgcttccaac gcagcgcgca tcggcaggcc ttgcgccagc aaccccgcga
     3541 ccgcggacgc gagcgtgcag ccggttccgt gaaactggcc ggccagccga ggccagcgcc
     3601 agctgtggcg cacatcccgc gcaaaccagg tattgaccac ctcgtccccg ccgccgtggc
     3661 ctcccttgat caatacgttc gggcaggcgc gcagcaaagc ttgcgcctgc acatcgattt
     3721 ccgattggcc cggacacagt cgcgccgcct cgggcaggtt cggcatcacc agcgttgcat
     3781 gcggcagcag gcttcgcacg ccatcgatcg ggtcgccgct ggacaaggca tctcccttgc
     3841 cgctcgccag gaccgtatcc agcaccacgg gcaagccggg atttcgatga tgcaagtgcg
     3901 caactatcgc ggcaatcgcc tccgcattcg gcaagctgcc gggaatgccg atcttcaccg
     3961 cggcaacgtc gatcttgtcg atcaaagcct gcgcctgccg cgtgaccagc tcggccgcca
     4021 ccgcgttgac gctgaagacc cgatcgttgt cctgcacggt aacggcggtg acgacggtga
     4081 gtgcatgggc gccctgggcc gcgatcgcct gcaggtcggc ctgcagaccg gcgccgccgc
     4141 tcgggtcgga gccggcaaac accaggacgc agcggcgcgt catgtcgcgc gcccctccat
     4201 cggggacgac ggcgacgcgg cgaagcgctt cgggatacgc ccggccagat aggcttcacg
     4261 gccggcctcg acagcaagct tcatcgcacg cgccatgcgc accgggtcct tcgcacctgc
     4321 gattgcggta ttcatcagca cgccgtcgca gccgagctcc atcgcgattg ccgcgtccga
     4381 ggcggtgccg acaccggcgt cgaccagcac cggcaccttc gcctgctcga tgatcagcga
     4441 caggttccag ggattcagga tgcccatgcc ggagccgatc agcgaagcca gcggcatcac
     4501 cgccacgcag ccgatttctt cgagcatctt cgcctggatc ggatcgtcgc tgcagtagac
     4561 catcacgtcg aagccatcgt tgaccagcgt ttcggcagcc ttcaatgtct ccggcatgtt
     4621 cggaaacagc gtgcgctcat cacccagcac ttctagcttg accagcttgt ggccgttcaa
     4681 cagctcgcgc gcgagctgca gcgtgtagac cgcatccttc gcgttgtagc agccggcggt
     4741 attgggcagg atcgtgtagt gcgaaggcgg caccacatcg agcaggttgg gcgcgttcgg
     4801 gtcctggccg atgttcacgc gacgaatcgc caccgtgatg atctcggcgt cgctcgcttc
     4861 ggtcgccagc cgcgtctgtt cgaggtcctt gtacttgccg ctgccgacca ggagtcggga
     4921 acggtaggtc ttgccggcga tggtcaagcc ttggtctggt gtgctttggt cgctcatggg
     4981 atccctttac tcttccattg gaagactcca ttcccccttt caggaggaag gagctctaga
     5041 taggacgttc aaccgccgcc gattgccttg acgacgtcca ccttgtcgcc tgcctgcagc
     5101 acacgctcgg cccagcgctg gcgcggcacc acctcgcggt tgatcgccac agccagcgac
     5161 ttgccagtca attccagcga ggcgatcaag tcctgcacgt tgaggttttc ggctaccgga
     5221 tgcggcgcgc cgttcaattc gatttccatc gtcattgctt gcgatacagt tcggcgccgg
     5281 atttcacgaa ctccaccgcc ttcacttcca ttcccttctt cagcgcatca tcttccgaaa
     5341 tgccctggga agccgcaaaa tcgcgcacgt cctgggtgat cttcatcgaa cagaaatgcg
     5401 gcccgcacat cgagcagaaa tgcgcgacct tggccgaatc cttcggcagc gtctcgtcgt
     5461 ggaattcgcg cgccttgtcc ggatccagcc cgagattgaa ctggtcttcc cagcggaact
     5521 cgaagcgcgc cttcgacagc gcgttgtcgc gcacctgcgc gccgggatgg ccctttgcca
     5581 ggtcggcggc gtgcgcggcc agcttgtagg tgatgatgcc ttccttcaca tcgtccttgt
     5641 tcggcaggcc cagatgctcc ttcggcgtca cgtagcacag catcgccgtg ccgtaccagc
     5701 cgatcatcgc tgcgccgatg ccggacgtga tgtggtcgta gcccggggcg atatcggtgg
     5761 tcaagggacc cagcgtatag aacggcgctt ccttgcactg ctcgagctgg atatccatgt
     5821 tttccttgat caggtgcatc ggcacgtggc cggggccctc gatcatcacc tgcacgtcgt
     5881 gcttccaggc gatctgcgtc agctcgccca gcgtcttcag ctcgcccagc tgcgcttcgt
     5941 cgttcgcgtc gtagatcgag cctggacgca ggccgtcgcc aagcgagaac gacacgtcgt
     6001 aggccttcat gatttcgcag atctcttcga aatgctcgta caggaaggat tccttgtgat
     6061 gcgcgaggca ccacttggcc atgatcgagc cgccgcgcga gacgatgccg gtcatgcgct
     6121 tggccgtcag cggtacgtac tgcaggcgta cgccagcgtg aatcgtgaag tagtcgacgc
     6181 cctgctcggc ctgttcgatc agcgtgtcgc ggaagatttc ccaggtcagc tcctctgcct
     6241 ttccgtgcac cttttccagc gcctggtaga tcggcaccgt gccgatcggc accgggctgt
     6301 tgcggatgat ccactcgcgg gtctcgtgga tgtgccggcc ggtcgacagg tccatcacgg
     6361 tgtccgcgcc ccagcggatc gcccaggtca tcttctcgac ctcctcgccg atcgagctgg
     6421 tgacggcgga gttgccgatg ttcgcgttga tcttgacgag gaagttgcgg ccgatcgcca
     6481 tcggctcggt ttcgggatgg ttgatgtt
//
LOCUS       DATLGF010000127         2151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_145949, whole genome shotgun sequence.
ACCESSION   DATLGF010000127 DATLGF010000000
VERSION     DATLGF010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145949"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VM406_03810"
     CDS             complement(<1..258)
                     /locus_tag="VM406_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75117.1"
                     /translation="MATIIAGRFQQQIEAEHMIAELLREGFSRQNIATFYLNPAGQHA
                     TYELGGDHDKSAGAKHSGVGVAAGVATGAVIGIAATPALGPL"
     gene            504..1400
                     /locus_tag="VM406_03815"
     CDS             504..1400
                     /locus_tag="VM406_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75118.1"
                     /translation="MQNTIIAVYDDYNDAEATMKELLLAGFSRTDVQLNPHADSTGVD
                     RASSVDEQTGGHAHGIGHFFRSLFGMEDTEEEEGRFRRDMYAEAIRRGSSLVTVHADR
                     DDQLEMALAILRRHNPVDLDKRSEHWRREGWSGFEESAPHYTGEQSRAERMRHAAASA
                     DPQAGPAGAGPASRSDAGLHVIRRRPAGGAAQEANARSARAAARADDFGDTVASADDA
                     DYRTHWENVYGRTGRRYEDYDAAYRYGALMGGSHGFRNYRWEDAEPRMRSDWEARNPG
                     SSWEQVRDAVRYGAEKGGGSIR"
     gene            complement(1408..1881)
                     /locus_tag="VM406_03820"
     CDS             complement(1408..1881)
                     /locus_tag="VM406_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVL75119.1"
                     /translation="MKFAHLIEINDPRNPLIDPLSRSQLWRGLVLRATQPEAFVPQLD
                     RCTLLEQSGLHLVRELQYGDIVLRDNVRFLPQQQVIYEVPAQEGLPPSRLTMTIEEPE
                     SGALFVRFEYETESSAEQDAVSAMYDDFRRSAYKESDLDTVRGIRELADAGRLGQ"
     gene            complement(1925..>2151)
                     /locus_tag="VM406_03825"
     CDS             complement(1925..>2151)
                     /locus_tag="VM406_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVL75120.1"
                     /translation="NMQMHAALASCLELARKLRRSSVAVGVESREDWALLRELGCTYA
                     QGWFIAAPMEAPALPAWSAQWSQFFRTALN"
BASE COUNT          398 a          677 c          730 g          346 t
ORIGIN      
        1 gaggggcccc aaggcgggcg tggcggcgat gccaatcact gcaccggtgg caacgcccgc
       61 cgcgacgccc acgccggagt gcttggcccc tgcggatttg tcatggtcac caccgagctc
      121 ataggtggcg tgctggccgg ccgggttcaa gtagaacgtt gcgatgttct ggcgcgaaaa
      181 tccctcgcgc agcaactcgg cgatcatgtg ttccgcctcg atctgctgct ggaagcgtcc
      241 tgcaatgatg gtggccatgg ctccctcctc ctagaaaaag cgtgcggtat gcaaattgag
      301 agcagccgtt gcacgaatag ttcttgcaga actgccacac accggcctgc gtcagcggca
      361 tgaaaaattc gccgcctgca cggtcgcacg gcgcgcacgc gccgtgcgag cacccgcgct
      421 cgggactggc acgtctcctg caagagatga ctattgccgg catgccgctt gcagccgaca
      481 gggtttttca tggagcgctg gaaatgcaga acacgatcat tgctgtgtac gacgattaca
      541 acgacgctga agcgacgatg aaggaattgc tgctggcggg cttttcacgc actgacgtgc
      601 agctcaatcc gcatgccgac agtaccggag tcgaccgcgc cagcagcgtg gacgagcaga
      661 ccggtggtca cgcccacggc atcggacatt tcttccggtc attgttcggc atggaggaca
      721 cggaggaaga ggaagggcgc ttccggcgcg atatgtatgc cgaggcaatc cggcgcggca
      781 gctccctcgt aacggtgcac gcggaccgcg acgatcagct cgagatggcg ctcgcgatcc
      841 tgcggcgcca taacccggtc gatctcgaca aacgttccga gcattggcgc cgcgagggct
      901 ggagcgggtt tgaggaaagt gcgccgcact acacgggaga gcaaagtcgc gccgagcgta
      961 tgcgccatgc ggccgcgagc gccgacccgc aggctggacc ggcgggagcg ggccccgctt
     1021 cgcgcagcga cgccggactg cacgtgatcc ggcggcggcc ggccggaggt gcggcgcagg
     1081 aggcgaatgc ccgaagcgcg cgagcggccg cccgcgcgga cgatttcggc gacaccgtcg
     1141 ccagtgccga cgacgccgat taccgcaccc actgggagaa cgtttacggt cgcacgggac
     1201 ggcgctacga ggactatgac gccgcctacc gttatggcgc cttgatgggc ggcagccatg
     1261 gattccggaa ctaccgttgg gaagatgccg agccgcggat gcgcagcgac tgggaagcgc
     1321 gcaacccggg cagcagctgg gagcaggtca gagacgcggt gcgctacggc gcggaaaaag
     1381 ggggcgggtc gatccgttag gtgcagccta ctggccgaga cgccctgcgt cggccagttc
     1441 ccggatgccg cgcaccgtgt cgaggtcgga ttccttgtat gccgagcgcc ggaaatcgtc
     1501 atacatcgcg ctgaccgcat cctgctcggc gctcgattcc gtttcgtatt cgaagcgcac
     1561 gaacagcgcc ccgctttccg gctcctcgat cgtcattgtc aggcgcgacg gcggcaagcc
     1621 ctcctgcgcc ggcacttcat agatcacctg ctgctgcggc aggaagcgca cgttgtcgcg
     1681 caagacgata tcgccatact gcagctcgcg taccaggtgc aggccggact gctcgaggag
     1741 cgtacaacgg tcaagttgcg ggacgaaggc ttcgggctgt gtcgcgcgca gcaccaggcc
     1801 gcgccagagc tgggagcggg agagcgggtc gatcagggga ttgcgcgggt cgttgatttc
     1861 tatcagatga gcaaacttca tggcggcaag ctgcgctgtg acacagcctt cgacggcaaa
     1921 gcgttcagtt tagcgccgtt cggaagaact gcgaccattg tgcgctccag gccggaagcg
     1981 ccggggcttc catcggcgcg gcaatgaacc agccttgcgc ataggtgcag ccgagctcgc
     2041 gcagcagcgc ccagtcctcg cgcgactcga cgcccacggc caccgagctg cggcgcagct
     2101 tgcgcgccag ctccaggcag gacgccagcg cggcgtgcat ctgcatgttt g
//
LOCUS       DATLGF010000128         4562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_37647,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000128 DATLGF010000000
VERSION     DATLGF010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37647"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..620
                     /gene="fur"
                     /locus_tag="VM406_03830"
     CDS             189..620
                     /gene="fur"
                     /locus_tag="VM406_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HVL75121.1"
                     /translation="MSSNPTDLKASGLKATLPRLKILDIFHNSPVRHLSAEDVYKTLL
                     AENMDIGLATVYRVLTQFEQAGLLHRNHFETGKAVFELNEGSHHDHLVCLDCGRVEEF
                     YDEEIEKRQQRIASERGFSIAEHALALYGHCHKTDCPHRTR"
     gene            675..998
                     /locus_tag="VM406_03835"
     CDS             675..998
                     /locus_tag="VM406_03835"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HVL75122.1"
                     /translation="MKIDTRSFARKPVRGRALIVLPNGAKWPAKPKDLSKDGISVVTS
                     EPLAMDVKCTVALETVCGSEIVHLMAQAKVIYCILSGTEGFRAGLQFTQIDSANNAVL
                     AQLLA"
     gene            complement(1040..2062)
                     /gene="hrcA"
                     /locus_tag="VM406_03840"
     CDS             complement(1040..2062)
                     /gene="hrcA"
                     /locus_tag="VM406_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HVL75123.1"
                     /translation="MQLDVRAQTLLKTLVERYIADGQPVGSRALSKLSGLDLSPATIR
                     NIMADLEEMGFVASPHTSAGRVPTPRGYRVFVDTLLTVQAIDESTVESKLQSRKQSTS
                     PQRIIANAAQVLSSLSQFAGVVMTPRHESVFQQIEFLRLSERRILLVIVAPNGEVQNR
                     LLPTETDYTPAQLVQASNYLNQHFAGCTFDQVRARLQNELRQLQNDMTRLMQAAVEAG
                     SDAMADAGEDVLISGERNLLSVSDLASNMNSLRKLFDLFEQKTSLVQLLDMSSKASGV
                     QIFIGGESQLVPVDAMSIVTAPYEANGRIVGTLGVIGPTRMAYERVIPIVDITAKLLS
                     NALSHS"
     gene            complement(2140..2847)
                     /locus_tag="VM406_03845"
     CDS             complement(2140..2847)
                     /locus_tag="VM406_03845"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HVL75124.1"
                     /translation="MPPSNTPVKAVLFDLDDTLWPIVPAIERAEALMYDWLERHVPAV
                     AREVTIESMRARRQALMETDPIYQIDLRRLRHAVLAEAFHQHGADPALVEHAMEVFSR
                     ARNEVQPFDDVVPVLTRLRQRYILGSVSNGVADLKAIGLAHFFHASVAAYQLGCAKPD
                     PEIFLTACRTLDISPEHAVYVGDDPLLDVKGAQRAGLRAVWLDRALHPRRPVPADLQP
                     DAVCADLHALHAWLESR"
     gene            2901..3818
                     /locus_tag="VM406_03850"
     CDS             2901..3818
                     /locus_tag="VM406_03850"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HVL75125.1"
                     /translation="MLPSQPHQLPAFKTVAVIGKYMAAGIEESLSELASFLASTGRTV
                     VFERETAQNLQLEPAAVTMTPDEIGRHADVAVVVGGDGTMLGIARQLAPYDVPLIGIN
                     QGRLGFMTDISQDVMIAALSDMLDGKLESERRTLLEGWVMRDGTTIFNALAFNDVVVS
                     RGSTSGMAELKVEVDDRFMYNQRSDGLIVATPTGSTAYALSAGGPLLHPSLTGIVLVP
                     IAPHALSNRPIVVPDSSAIEIEIMGGRDISVNFDMQSLASLQHHDRILIKRSCHTTTF
                     LHPQGWSYYDTLREKLHWHEYPSVAGQLK"
     gene            3848..>4562
                     /locus_tag="VM406_03855"
     CDS             3848..>4562
                     /locus_tag="VM406_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVL75126.1"
                     /translation="MLRTLSIRDFVIVDAIELEFAPGFTVFTGETGAGKSILIDALAL
                     ALGGRGDASVVREGAAKTEISAEFDVAPDTAAWLDEHEFSAEDGTVLLRRVIDNAGRS
                     RAFINGSSATVAQLREVGEMLIDIHGQHAHQSLLRADAQRMLLDSQAGLQDQAKEVAQ
                     AYKAWRALARQREQFEADARNVLLERERLEWQVGELDKLAPKAGEWAEVSNEHSRLSH
                     AASLIEGAQEALDAISESDD"
BASE COUNT          832 a         1449 c         1488 g          793 t
ORIGIN      
        1 ctcttccatc aatggattct tgccagcgca tcccgtcaag agcacggcgg cggccaccag
       61 gacggtgccg gcgccaaagg cgcgttcagg acggaaaaac agcatacgca taagcggtct
      121 cgattcgttg ggcaagcagg ccaaaaggct atatgataaa gcagatattc cggaatcgac
      181 catcgataat gagcagcaat cccaccgatc tgaaagccag cggtttgaag gcaaccctgc
      241 cgcggctgaa gattctcgac atcttccaca acagtccggt acgccacctc agcgccgaag
      301 acgtctacaa gacgctgctg gccgagaaca tggatatcgg cctggcgacc gtctaccgcg
      361 tactcacgca attcgagcag gccggcctgc tgcatcgcaa ccacttcgag accgggaagg
      421 cggttttcga actgaacgaa gggtcgcatc acgaccacct ggtctgcctc gattgcggcc
      481 gcgtcgaaga gttctacgac gaggagatcg aaaagcgcca gcagcgcatc gccagcgagc
      541 gcggattctc gatcgccgaa cacgctctgg cgctctacgg ccactgccac aagacagact
      601 gcccgcatcg cacacgctga tccccgatcc aggcggccct gccggcccgc ccacatctgc
      661 cggcattccg attcatgaag atagatacac gcagtttcgc gcgcaagccg gtcagggggc
      721 gcgcgttgat cgtccttccg aacggggcca agtggcccgc caagccgaag gatttgtcca
      781 aggatggcat ctccgtcgtc acgtccgaac cgcttgcgat ggacgtgaag tgcacggtgg
      841 cgctcgagac cgtctgcggt agcgagatcg tccacctgat ggcccaggcc aaggtgatct
      901 actgcatcct gagcgggacc gagggttttc gcgcgggact ccagttcacc cagatcgatt
      961 cggcaaacaa cgcggtcctt gcgcagttgt tggcgtagtc tgcccggcag tcgggcggcc
     1021 agcctggtga gtttgtcgcc taagagtggc tgagggcgtt ggacaacagc ttggccgtga
     1081 tgtcgacgat gggaatcacc cgctcgtatg ccatccgggt cgggccgatc acgccaagcg
     1141 tgccgacgat cctgccattc gcctcgtacg gtgcggtgac gatgctcatc gcatcgaccg
     1201 gcaccagctg ggattccccg ccgatgaaga tctgcacgcc gctcgccttg ctcgacatgt
     1261 cgagcagctg gacgaggctg gtcttctgct cgaacagatc gaacagcttg cgcagcgagt
     1321 tcatgttgga tgcgaggtcg gacacgctga gcaggttgcg ctctccggag atcagcacat
     1381 cctcgccggc gtcggccatt gcgtcgctgc cggcttcgac ggcggcctgc atcagccggg
     1441 tcatgtcgtt ctgcagctgg cgcagttcgt tttgcagacg cgcgcgcacc tggtcgaagg
     1501 tgcagccggc aaagtgctgg ttcaggtagt tggaggcctg tacgagctgg gccggggtgt
     1561 agtcggtctc ggtgggcagc agccggttct ggacttcgcc gttcggcgcc acgatcacca
     1621 gcaggatgcg acgctccgac aggcgcagaa actcgatctg ctggaatacc gactcgtgcc
     1681 gcggcgtcat caccacgccg gcgaactgcg acagcgagga cagcacctgc gcggcattgg
     1741 caatgatgcg ctgcggcgac gtgctctgct tacgcgactg cagtttcgat tccaccgtgc
     1801 tttcgtcgat tgcttgcacg gtgagcaagg tgtctacgaa gacccggtac ccgcgcggcg
     1861 tcggcacgcg tccggccgag gtgtgcggac tggccacgaa gcccatctct tccaggtcgg
     1921 ccatgatgtt gcgtatggtc gccggagaaa gatcgaggcc tgagagtttc gacaaggcgc
     1981 gcgagccgac cggctggccg tcggcaatgt atcgctcaac gagggtcttg agcagggtct
     2041 gggcgcggac gtcgagttgc atgcacgtat tttagccagc gtaatgggcc gccgccactc
     2101 ggctgtgtcc gaatcgcgca actccgtgct tgcgctggct cagcggctct cgagccaggc
     2161 atgcaaagcg tgcaagtcgg cgcagacggc atcgggctgc aggtctgccg gaacggggcg
     2221 ccgcggatgc agtgcacggt ccagccatac cgcgcgcagg ccggcccgct gcgcgccctt
     2281 gacgtcgagc aaggggtcgt ctccgacgta caccgcgtgc tccggagaga tgtcgagcgt
     2341 gcggcaggct gtcagaaaga tctcgggatc gggcttcgcg catccgagct ggtaggcggc
     2401 tacggacgca tggaagaagt gcgccagtcc tattgccttc aggtcggcca cgccgtttga
     2461 caccgaaccg aggatgtaac gctgccgcag ccgcgtgagc accggcacca cgtcgtcgaa
     2521 gggctgcact tcgttgcgcg cgcgcgagaa cacctccatc gcgtgctcga ccagcgcagg
     2581 gtcggcgccg tgctggtgga aggcctcggc cagcacggca tggcgcaggc ggcgcaggtc
     2641 gatctggtag atcggatcgg tttccatcag tgcctgccgg cgtgcgcgca tgctctcgat
     2701 cgtcacttcg cgcgcgaccg cgggcacgtg gcgctcaagc cagtcgtaca tgagcgcctc
     2761 ggcgcgctcg atggcgggga cgatgggcca gagggtgtcg tcgaggtcga acaggacggc
     2821 tttgacgggg gtgttcgacg gcggcatggg cggctcgggc aagctgtgac ggacagacga
     2881 attatggtct aatctcggcc atgttgcctt cccagccaca ccagcttcct gcgttcaaga
     2941 ccgttgccgt catcggcaag tacatggccg cgggcatcga ggaatccctc tcggaactcg
     3001 cctccttcct cgccagcacc ggccgcaccg tggtgttcga gcgcgaaacc gcacagaatc
     3061 tccagctcga gccggcggcc gttacgatga ctcccgacga gatcggtcgc catgccgacg
     3121 tcgcggtggt ggtcggcggc gacggcacca tgctcggcat cgcgcgtcag cttgcccctt
     3181 acgatgtccc gctgatcggc atcaaccagg ggcgtctggg tttcatgacc gacatcagcc
     3241 aggacgtgat gatcgccgcg ctgtccgaca tgctcgacgg caagctggaa tccgaacggc
     3301 gcacgctgct cgagggttgg gtgatgcgcg acggcacgac gatcttcaat gcgctcgcgt
     3361 tcaacgacgt ggtggtgtcg cgcggctcga cctccggcat ggccgagctg aaggtcgagg
     3421 tcgacgaccg cttcatgtac aaccagcgct ccgacggcct gatcgtcgct acgcccaccg
     3481 gttccaccgc ctatgcgctg tcggccggcg gtccgctgct tcaccccagc ctgaccggca
     3541 tcgtgctggt gccgatcgcg ccgcatgcgc tgtcgaaccg gccgatcgtg gtgccggaca
     3601 gcagcgccat cgagatcgag atcatgggcg ggcgcgatat cagcgtgaat ttcgacatgc
     3661 agtcgcttgc cagtctgcag caccatgacc gcatcctcat caagcgttcc tgccacacga
     3721 cgacattcct gcatccgcag ggatggagtt actacgacac gctgcgcgag aagttgcact
     3781 ggcatgaata tccgtccgtg gccggacagt taaaatagcc ggtcatttcc cagcacccct
     3841 gtccgacatg ctgcgcacgc tatccatacg cgactttgtg atcgtcgacg cgatcgaact
     3901 cgagttcgcg ccgggcttca ccgtttttac cggcgaaacc ggtgccggca aatcgatcct
     3961 gatcgatgcc ctggcgctgg cgctgggcgg gcggggcgat gccagcgtgg tgcgcgaggg
     4021 cgcggcgaaa accgagatca gcgcggagtt cgatgtcgcg ccggataccg ccgcctggct
     4081 cgacgaacac gagttttccg ccgaagacgg gactgtgctg ctgcgtcgcg tgatcgacaa
     4141 cgcgggccgc tcgcgcgcct tcatcaatgg cagcagcgcc accgtcgccc agctgcgcga
     4201 ggtcggcgaa atgctgatcg acatccacgg ccagcatgcg catcagtcgc tgctgcgggc
     4261 cgatgcccag cgcatgctgc tcgacagcca ggccgggctg caggaccagg cgaaggaggt
     4321 cgcacaggct tacaaagcct ggcgggcgct cgcgcgccag cgcgagcagt tcgaggccga
     4381 tgcccgcaac gtcctgctcg agcgcgagcg cctggaatgg caggtcggcg aactcgacaa
     4441 gcttgcgccc aaggcaggtg aatgggccga ggtcagcaac gaacacagcc ggctgtcgca
     4501 cgcggccagc ctgatcgagg gggcgcagga ggcgctggac gcgatctcgg agtccgacga
     4561 ca
//
LOCUS       DATLGF010000129         9952 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_66676,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000129 DATLGF010000000
VERSION     DATLGF010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 9952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9952
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66676"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VM406_03860"
     CDS             complement(<1..544)
                     /locus_tag="VM406_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVL75127.1"
                     /translation="MTLLNIPFDLIDGPTWLSEAARERVGRHNAMRQVPTLVLPDGEI
                     LTESAAILMYLADAHPEARLAPKADEASRRQFLRWMFFVSSAIYSLHWIKPDVGRVGA
                     PQSARDGVVQAVHERIAFCWRTMDAQLNPQKYLLGDDLTVLDLYVAVVSRFGPWRERF
                     YEEAPRMTPAIRRVDEEPRLR"
     gene            826..2517
                     /locus_tag="VM406_03865"
     CDS             826..2517
                     /locus_tag="VM406_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HVL75128.1"
                     /translation="MDLFSRLARFFPFLRWPRPTLATLRADAWAGITVGLVLVPQSLA
                     YATLAGMPPHTGLYAALVPAVVGALWGSSPLLAVGPVALTSLLVFGALAPLAQPGSAQ
                     WVELAIWLALYAGLMQFALGAFRLGRIADLVSHPVVLGFINAAAVIIILTQLPALLGL
                     SVPAGASWTQAHFDPATAAFGLAAVALLFLFRRFLPRLPGILIVTIVGIVASAVFGYA
                     EAGGAVVGAIPAGLPAPVLPSAIPFDVHRQLLPAALILALVSFTEAMSSCRALARRRN
                     EQWDESQELVGQGMAKIAAAFSGAFPVSGSFSRSALNLYAGAVSAWSTLFATACVLLS
                     LLFLTEYLYDLPFAVLAAIIIVPVLNLIDVAGLRRLLKVSRNDGVVALVTFAATLAAL
                     PDLYWGVAAGVGLNMVVFLYRRMHPRIIEVALHPDGTLRDSARFGLPRLAPDVIAVRM
                     DSALNFLTGNVLERFVLGRCREDRSIRRVLLCSNAWNDIDASGIDTLEALRFALEVEG
                     VELYVSAVKIQVWSVMDRAGFAERLGKARVFATDREAVDALSGEPDASTTASPAS"
     gene            complement(2758..4350)
                     /locus_tag="VM406_03870"
     CDS             complement(2758..4350)
                     /locus_tag="VM406_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HVL75129.1"
                     /translation="MMPTQSLQPASTTPSFLRVFSPRADNIAPLVILLLLLHLALWTV
                     FTGLSHRSPDWDNMEELVWAGGLEWGYYKHPPLPTWVLYGLVSVFGRPVWLTFFTGQL
                     SVVLALWMVWKLGCEMTTPRRALIATLFVSLVAYFTVRGVMNNHNTMQLWSVAGAIWM
                     LYRATRYDSMRAWALLGVFSACAFLTKYSVLVQFATFFLYLLFTGQLRRARTWKGIAL
                     ATAVLLVLVAPHLIWLARQAGGPLTYAENAMAPLTTYWQVLGDQFDFTLTNLGRLAVV
                     GLAVALVAVWQRRQLRTTPAAARPPKMARSLAASDRFFLLFVGFAPLLLTMTVAGAMK
                     IPLLAHWPTTFFMLFGFLPFWLLRSDDDAALLRKTMIVVITLQVLGAALYGVARGPLA
                     DHAGRPSRATFPGAEVARLVHAEWTSRMQAPLRVVAADTWLGGNIATRIGRDVQVLID
                     GDFGKAPWVDRESAQCGMLVAINRSPANPDRTDEKVVALMEQAAHRGTVEVPWTRKTG
                     GPVVVVEWGIIPAGADCAAAGR"
     gene            4506..5510
                     /locus_tag="VM406_03875"
     CDS             4506..5510
                     /locus_tag="VM406_03875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVL75130.1"
                     /translation="MVTSTALARKRPADALFSCVIPAYNEAPHLARFLQELRATLDTF
                     ATRYEIIVVNDGSSDNTEQEIAPFLGQSGLRYISLSRNFGKEAALSAGIDAAKGDAVI
                     LMDADFQHPLELLPQMAQKWWEGIDMVYGVISDRSAESQLKRWGTGMFYQLMESGAPI
                     KIPRNAGDFRLMDRSVVNALRQLPERNRFMKGLYAWVGFSSVALPFVPNRRATGVSSF
                     SYRGLSRLALAGVTAFTTLPLRVWSAVGVAVSIFAILYAFWIAGTTLLFGNDVPGWAT
                     LAAGLMFFSGVQLISIGVLGEYLGRVYDEVKKRPLYIVAHDVDNGPHKPAAEGLPPCT
                     "
     gene            5501..5911
                     /locus_tag="VM406_03880"
     CDS             5501..5911
                     /locus_tag="VM406_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HVL75131.1"
                     /translation="MHLKELLRMLRFGSVGLLAAAVHYWVVIALVELAGIAPMRANVG
                     AFAVAFWVSYFGHRHWTFSDTVATHAPGAHSSLPRFLAVALLGFCMNAILFYLLLQYA
                     GWPYYVSLAVVVFAVAAMTYLLSRLWAFRRAVRT"
     gene            5908..6717
                     /locus_tag="VM406_03885"
     CDS             5908..6717
                     /locus_tag="VM406_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HVL75132.1"
                     /translation="MTHTTIPVALCADDYAQHPGIDAGILALLGLGRLTAVSCFATSP
                     RWQTVSAPALRAHRAQADIGLHFNLTEGFGKPMPSLHAVILRSLLRRLDPADVEQALT
                     EQLDAFEAGFGQPPDFIDGHQHVHQLPGVREVVLKLIRQRYPGHPVWVRNTVPADRSW
                     RGKPQVLKLLGGQSLAAALKRARIASNDGFAGVYGFDKADYASSFKAWLERARPGMLI
                     MCHPAAQAYPNDEIAQQRVVEYRFFTSPRFDEMLAATRTSLVRLSRIASNV"
     gene            complement(6721..7626)
                     /locus_tag="VM406_03890"
     CDS             complement(6721..7626)
                     /locus_tag="VM406_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007736323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL75133.1"
                     /translation="MATNVTLRQLRYFVAAAQSGQFSMAATSEHVSQSAITNAVLALE
                     QELGVRLFDRHPHGVTLTAEGEDFLHHAHHILDSVQDAMHKPRFGARELRGTVRIAAS
                     YTVLGYFLPELLARFRASYPEIEIDLRDMERPEIEEAVQAGAVDVGVAILSNVTRVGR
                     FGHAVLIRSRRQLWVAPSHPLAALPSPALKDIAAYPYIMITADEGEASALRYWKARRL
                     EPNIGFRTTSIEAVRGLVAHGFGVTILSDMVFRPWSLEGKRIEARPILDAVPQMDVGI
                     LWNKAGKLSRPAELFQQFLIHAYGH"
     gene            7721..>9952
                     /locus_tag="VM406_03895"
     CDS             7721..>9952
                     /locus_tag="VM406_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HVL75134.1"
                     /translation="MNAPITPDMQSDMQKALAAATIDDKYLVEKGRAYLSGTQALVRL
                     PMLQHELDRRSGLDTAGYISGYRGSPLGGLDMALWKAKKYLQQRNIVFQPGVNEDLAA
                     TAVWGTQQTNLWGTGKHDGVFGMWYGKGPGVDRSGDVFKHANSAGTAPHGGVLALAGD
                     DHAAKSSSVAHQSEHALIAAGIPVLYPSNVQEYLDYGLHAWAMSRFSGLWVGMKCVTD
                     VVESSASVEVDPDRVSIVRPDDFEMPEGGLNIRWPDPPLAQEARLYDYKWYAALAYIR
                     ANKLNRVVVDSPNARFGIITAGKAYLDVRQALADLGLDDEACARVGIRVFKVGCVWPL
                     DAQDARAFAEGLQEILVVEEKRQILEYALKEELYNWREDVRPRVFGKFDERGNEGGEW
                     SVPRGNWLLPARYELSPAIIARAVAKRLAGLDLPEDIRSRIAARLAVLDAAETVTAPK
                     PAEERKPWFCSGCPHNTSTRVPEGSRALAGIGCHYMAMWMDRKTETFTQMGGEGVSWL
                     GQMHFSNDRHVFVNLGDGTYFHSGHLAIRAAVAAGASVTYKILYNDAVAMTGGQPVDG
                     VLTVPQIAQQLIAEGVKKLVIVTDEPEKYTPQVKLPEGVPVFHRDELDRVQIELRETP
                     GTTVLIYDQTCATEKRRRRKRGTYPDPARRLFINDAVCEGCGDCSVQSNCLSVEPLAT
                     PFGTKRRINQSSCNKDFSCAKGFCPSFVAAEGAQPRRPQGAGKGVSQPDFAALPLPNL
                     PDLA"
BASE COUNT         1666 a         3294 c         3285 g         1707 t
ORIGIN      
        1 cccgcagccg cggctcctcg tcgacgcgcc tgattgccgg cgtcatgcgc ggcgcctctt
       61 catagaagcg ctcgcgccac gggccgaagc gggagaccac ggcgacatag agatcgagaa
      121 ccgtcaagtc gtcgccaagc aggtacttct gcggattcaa ctgggcatcc atcgtgcgcc
      181 agcagaaggc gatgcgctcg tggaccgcct gaaccacacc gtctcttgcg ctttgcggtg
      241 cgccgacccg accgacgtcc ggcttgatcc aatgcagcga gtagatcgcg gatgaaacga
      301 agaacatcca gcgaaggaac tgcctgcgcg aggcttcatc cgcttttgga gccagcctgg
      361 cttcggggtg cgcatcggcg aggtacatca ggatcgcggc actctcggtg agaatctccc
      421 cgtccggcag gaccagcgta ggaacctggc gcatcgcgtt gtgcctgcca acgcgttcgc
      481 gagccgcttc cgagagccag gtggggccat cgatgagatc gaacgggatg ttcagaagcg
      541 tcaacgccgc ttccacggca acggagcccg aggcggctgc gccataaagc ctgagttttt
      601 cagcttggtg cattgagagg gtggatgtca aggtcctaag gtagtttgca acgaaggtca
      661 ggcagcgcca aacgctcgac ccaggaactt gtggcgagat attcgtccaa tcaggtcgtc
      721 ggcggcaaga ctgatgggta tttggtttgt gtttccatca tgcaagccag aatagtgagc
      781 atgtgataag gtcacccgcc tgatccgaac acgccgcctc acccgatgga cctcttctcc
      841 cggcttgccc gcttcttccc gttcctgcgc tggccgcgtc ccacgctggc caccttgcgc
      901 gccgacgcct gggccggcat cactgtggga ttggtactgg taccgcagtc gctcgcctat
      961 gcaacgctgg ccggcatgcc gccgcatacc ggcctctatg cggcgctggt gccggccgtg
     1021 gtgggtgcgt tgtggggatc gtcgccactg ctggcggtcg ggccggtcgc actgaccagt
     1081 ctgctggtgt tcggcgcgct ggcgccgctg gcacagccgg gcagcgcaca gtgggtggag
     1141 ctggcgatct ggctggcctt gtacgcaggc ctgatgcagt tcgcgctcgg tgcattccgg
     1201 ctcggccgca tcgccgatct ggtgtctcat ccggtggtgc tcggcttcat caacgcggcc
     1261 gcggtgatca tcatcttgac gcagctgccg gcactgcttg gcttgagcgt gccggccgga
     1321 gcaagctgga cgcaggcgca tttcgatccc gccacggcgg cgttcggcct cgcggccgtt
     1381 gcgctgctgt tcctgttcag gcgctttctg ccgcgcctgc ccggcatcct gatcgtcacg
     1441 atcgtcggca tcgtcgccag cgcggtgttc ggctatgccg aggcgggcgg tgcagtggtg
     1501 ggcgcgattc cggccggatt gccggcgccg gtgctgccgt cggcgatccc gttcgacgtg
     1561 catcgccagc tgctgccggc cgcgctgatc ctcgcactgg tcagcttcac ggaggcgatg
     1621 tcgagctgcc gtgcactggc gcggcgacgc aacgagcaat gggacgagag ccaggaactg
     1681 gtcggccagg gcatggcaaa gatcgccgcc gctttctccg gcgccttccc ggtcagcggc
     1741 tcgttctcgc gctcggcgct caatctgtat gccggcgcgg tcagtgcgtg gtcgacgctg
     1801 ttcgcgacag cctgcgtgct gctgagcctg ctgttcctga ccgagtatct gtacgacctg
     1861 ccgttcgcgg tgctggctgc gatcatcatc gtgccggtgc tgaacctgat cgacgtggcc
     1921 ggactgcgcc gcctgctcaa ggtctcgcgc aacgacggcg tcgtcgcgct cgtcaccttc
     1981 gccgcgacgc tggcggccct gcccgatctg tactggggtg tggccgcggg tgtcgggctg
     2041 aacatggtgg tgttcctgta ccggcgcatg catccgcgca tcatcgaagt cgcactgcat
     2101 ccggacggca cgctgcgcga cagcgcgcgt ttcggtttgc cgcggctggc gccggacgtc
     2161 atcgctgtgc gcatggattc ggcgctgaac ttcctgaccg gcaacgtgct ggaacgcttc
     2221 gtgctcggcc gctgccgcga ggaccgcagc atccgccgcg tgctgctgtg ctcgaacgcc
     2281 tggaacgaca tcgacgccag cggcatcgac acgctggagg cgctgcgctt cgcgctggag
     2341 gtcgagggcg tcgagctgta cgtcagcgcg gtcaagatcc aggtctggag cgtgatggat
     2401 cgcgcgggat tcgccgagcg cctcggcaag gcgcgcgtgt ttgcaacaga ccgcgaggcg
     2461 gtcgacgcac tctcgggcga gccggatgcg agcacgaccg cttcgcctgc atcgtagcat
     2521 cggcaaacaa ccgcttcctg cgagggacag cccatgaaca ccccgctgcg caagctagac
     2581 ctgacctcgc tgcgcctgtt cgtcgcggtg tgccgagcag aacatcgcac acgccgctga
     2641 acgcgaactg atcgccgtcg gcggggagcc ggcgcatcga agcggtgttc gaggcggggc
     2701 actggttgta ttcggatgag gatgggatcg ttctggcgcg tgcgcgcttg cagacgatta
     2761 acggcccgcc gccgcgcagt cggccccggc cggaatgatg ccccactcga cgaccaccac
     2821 cgggccgcct gtcttgcgcg tccacggcac ttcgaccgtg ccccggtgcg cggcctgctc
     2881 catcagcgcg acgacctttt catcggtgcg gtccggattg gccgggctgc ggttgatggc
     2941 caccagcatc ccgcactgcg cgctttccct atccacccag ggcgccttgc cgaagtcgcc
     3001 gtcgatcagg acctgcacgt cgcggccgat gcgcgtcgca atgtttcccc ccagccaggt
     3061 gtccgcggcg accacgcgca gcggagcctg catgcggctc gtccactcgg catgcaccag
     3121 gcgcgccact tcggcgccgg ggaaggtggc ccggcttgga cgcccggcat ggtcggccag
     3181 cggaccgcgc gcgacgccgt agagggcggc gccgaggacc tgcagcgtga tcacgacgat
     3241 catcgtcttg cgcagcaagg ccgcatcatc gtcgctgcgc agcagccaga acgggagaaa
     3301 gccgaacagc atgaagaagg tcgtcggcca gtgcgcaagc agcggaatct tcattgcgcc
     3361 ggccaccgtc atggtcagca gcagcggcgc gaagccgacg aacagcagga agaagcggtc
     3421 ggatgcggcc agcgagcggg ccatcttcgg cgggcgggcc gcggccggcg tcgtgcgcag
     3481 ctgccggcgc tgccagaccg cgaccagcgc gaccgccagg cccacgacgg cgagccggcc
     3541 gagattggtc agcgtgaaat cgaactggtc gccaagcacc tgccagtagg tcgtcagcgg
     3601 cgccatcgcg ttttccgcat aggtcagcgg tccgccggcc tggcgggcaa gccagatcag
     3661 gtgcggcgcg accagcacaa gcaggacggc ggtcgccagc gcaatgccct tccacgtccg
     3721 cgcccgccgc agctgcccgg tgaacagcag gtacaggaag aacgtggcga actggacaag
     3781 cacgctgtac ttggtcagga aggcgcaggc ggaaaacacg ccgagcaggg cccaggcgcg
     3841 catgctgtcg tagcgggtgg cgcgatacag catccagatc gcgccggcga ccgaccacag
     3901 ctgcatcgtg ttgtgattgt tcatcacccc gcgcaccgtg aagtacgcga ccagcgagac
     3961 gaacagcgtg gcgatcagcg cgcgcctggg cgtggtcatc tcgcaaccga gtttccagac
     4021 catccacagc gccagcacga cactgagctg gccggtgaag aaggtcagcc agaccgggcg
     4081 gccgaatacc gataccagcc cgtacagtac ccaggtcggc aacggcgggt gcttgtaata
     4141 gccccattcc agcccgccgg cccagaccag ttcttccatg ttgtcccagt cgggagaacg
     4201 atggctcagg ccggtgaata ccgtccacaa ggccagatgc agcaatagca ggagaatgac
     4261 gaggggcgcg atgttgtcgg ccctgggcga aaacactctc agaaacgaag gcgtcgtcga
     4321 ggcgggttgg agcgattgag tcggcatcat ctggtgcacc gggaaggtcg tatcgtggat
     4381 cgtgttgcgg cgcctgaatg cgggccgggc gcgccgtcgg cgacagggcg gtgaattata
     4441 atcgctgacc ctggcgccgc tcggacgcgg cgccgctttg caacttgaca gaatcgcact
     4501 ccctcatggt gacatcgacc gcactcgcga ggaaacgtcc cgccgatgcg ctgttttcct
     4561 gcgtgatccc cgcctataac gaagcaccgc acctcgcccg gtttctgcag gaattgaggg
     4621 ccacgctcga cacgtttgcg acgcgctatg aaatcatcgt cgtcaacgac ggcagcagtg
     4681 acaacaccga gcaggagatt gcgcccttcc tcggccagag cggactgcgc tatatcagcc
     4741 tgtcacgcaa cttcggcaag gaagcagcct tgtccgccgg tatcgatgcg gccaagggtg
     4801 acgcggtgat cctgatggat gccgatttcc agcatccgct cgaactgttg ccgcaaatgg
     4861 cgcagaagtg gtgggagggc atcgacatgg tctacggcgt gatcagcgac cgcagcgccg
     4921 aatctcagct caagcgctgg ggcaccggca tgttctatca actgatggaa tccggcgcgc
     4981 cgatcaagat cccgcgcaat gcaggcgatt tccgtttgat ggacaggagc gtcgtcaatg
     5041 ccttgcgcca gctgcccgag cgcaaccgct tcatgaaagg cttgtatgcc tgggtcggct
     5101 tcagcagcgt cgcgctgccg ttcgtgccga accggcgcgc aaccggcgtg tcgagtttca
     5161 gctaccgcgg cctgagccgc ctggcgctgg ccggcgtgac cgctttcacc acgctgccgt
     5221 tgcgcgtctg gagcgcggtc ggcgtcgcgg tctcgatctt cgcgatcctg tacgcgttct
     5281 ggatcgccgg caccacgctt ttgttcggca acgacgtgcc gggctgggca acgctggccg
     5341 ccggcctgat gttcttttcc ggcgtgcagc tgatctcgat cggcgtactc ggcgaatatc
     5401 tcgggcgcgt ctatgacgaa gtcaagaaac ggccgctcta catcgtcgcc cacgacgtcg
     5461 acaatggccc gcacaagccg gccgccgagg gcctgcctcc gtgcacctga aggaactgct
     5521 gcgcatgctg cgcttcggca gcgtcggtct gctggccgct gccgtgcatt actgggtggt
     5581 gatcgccttg gtcgagctgg ccggcatcgc gccgatgcgc gcgaatgtcg gcgcgtttgc
     5641 ggtcgcgttc tgggtcagtt atttcggcca ccggcactgg acgttttccg ataccgtcgc
     5701 cacccacgcg cccggtgcgc attcctcgct gccgcgcttt ctggccgtcg cattgctcgg
     5761 gttctgcatg aacgcgatcc tgttctatct gctgctgcaa tacgccggct ggccgtacta
     5821 cgtctcgctc gcggtcgtgg tgttcgcggt ggcggccatg acttacctgt tgagccgcct
     5881 gtgggcattc cgccgcgcgg tccgcacatg acacacacca ccattccagt cgccttgtgc
     5941 gcggacgatt atgcgcagca ccctggcatc gacgccggca tcctcgcgct gctgggcctg
     6001 ggacgcctga ccgcggtcag ctgctttgcc acctcgccgc gctggcagac ggtatcggcg
     6061 ccggcgctgc gtgcgcatcg cgcgcaggcc gatatcggcc tgcatttcaa cctgaccgaa
     6121 ggtttcggca agccgatgcc gagcctgcac gcggtgatcc tgcgcagcct gctgcgccgc
     6181 ctggacccgg ccgacgtgga acaggcgctg accgagcagc tcgatgcgtt cgaggccggc
     6241 ttcgggcagc cgccggactt catcgatggg catcagcatg tgcaccagtt gccgggcgtg
     6301 cgcgaggtcg tgctgaagct gatcaggcag cgctatcccg gccatccggt ctgggtgcgc
     6361 aatacggtgc cggcagaccg ttcgtggcgc ggcaagccgc aggtgctgaa acttttgggc
     6421 ggccagtcgc tggccgcggc attgaaacgc gcgcgcatcg ccagcaatga cggcttcgcc
     6481 ggcgtctacg gcttcgacaa ggccgattac gcttccagct tcaaggcgtg gctggagcgg
     6541 gcacggccgg gcatgctgat catgtgccat ccggccgcgc aggcgtatcc gaatgacgag
     6601 attgcgcagc agcgcgtggt cgagtaccgc ttcttcacgt ccccccgatt cgacgagatg
     6661 ctggccgcca cgcggaccag cctggtgcgg ctgtcgcgaa ttgcgtcgaa cgtataagcg
     6721 tcagtgcccg tacgcgtgaa tgaggaactg ctgaaacagt tccgccggcc ggctgagctt
     6781 gcccgccttg ttccacagaa ttcccacatc catctgcggc accgcatcga gaatcggccg
     6841 cgcctcgatg cgcttgcctt ccagcgacca cgggcgaaac accatgtccg acaggatcgt
     6901 cacgccgaaa ccatgggcga ccaagccgcg caccgcctcg atcgaggtgg tgcgaaatcc
     6961 gatattcggt tccagccgcc gcgccttcca gtagcgcagc gccgacgctt ctccttcgtc
     7021 ggcggtgatc atgatgtacg gataggcggc gatgtccttc agtgctggcg agggcagtgc
     7081 ggccaacgga tgcgagggcg ccacccatag ctggcggcgc gagcggatca gcaccgcgtg
     7141 gccgaagcgg ccgactcgcg tgacgttcga caggatcgcc acgccgacgt cgaccgcgcc
     7201 ggcctgcacc gcctcttcga tctccggccg ctccatgtcg cgcaggtcga tctcgatctc
     7261 cggatagctg gctcgaaagc gcgccagcag ttcgggcagg aaatacccga gcaccgtgta
     7321 ggacgcggcg atgcgcaccg tgccgcgcaa ttcgcgcgcg ccgaagcgcg gtttgtgcat
     7381 cgcatcctgc accgagtcga ggatgtggtg tgcgtgatgc aggaagtcct cgccctcggc
     7441 ggtcagcgtc acgccgtgcg ggtggcggtc gaacagccgt acgcccagct cctgttccag
     7501 cgccagcacc gcattcgtga tcgccgactg cgatacgtgt tcgctggtgg ccgccatcga
     7561 gaactggccg ctttgcgccg ctgcaacgaa gtagcgcagc tgccgcaacg tgacattggt
     7621 cgccatgcgt ttatcgaata ccggtgttga gaatttgtga ttttacgatg gaccacccca
     7681 aaaatatact cgggcgcatc gttgaaaaag gagacacgcg atgaacgcgc ccattacgcc
     7741 cgacatgcag tccgacatgc aaaaggcgct cgccgccgcc acgatcgacg acaagtatct
     7801 ggtggaaaaa ggccgcgcct acctgagcgg cacccaggcg ctggtgcgcc tgccgatgct
     7861 gcagcatgaa ctggaccggc gcagcgggct cgacaccgcc ggctatatct ccggctaccg
     7921 cggctcgccg ctcggcggac tcgacatggc gctgtggaag gcgaagaagt atctgcagca
     7981 gcgcaacatc gtgttccagc ccggcgtcaa cgaagacctc gccgccaccg cggtgtgggg
     8041 cacccagcag accaatctgt ggggtaccgg caagcatgac ggcgtgttcg gcatgtggta
     8101 cggaaaaggg ccgggcgtgg accgcagcgg cgacgtgttc aagcacgcga actcggccgg
     8161 caccgcgccg cacggcggcg tgctcgcgct ggccggcgac gaccatgcgg ccaaatcctc
     8221 ctcggtcgcg caccagtccg aacacgcgct gatcgccgcc ggcattccgg tgctgtatcc
     8281 gtcgaacgtg caggaatacc tggactacgg cctgcacgcg tgggcgatga gccgcttctc
     8341 cggcctttgg gttggcatga agtgcgtgac cgacgtggtc gaatccagcg cctccgtcga
     8401 agtggacccg gaccgcgtgt ccatcgtgcg cccggacgat ttcgagatgc ccgaaggcgg
     8461 gctgaacatc cgctggccgg acccgccgct ggcgcaggag gcgcgcctgt acgactacaa
     8521 gtggtatgcc gcgctcgcct acatccgtgc caacaagctc aatcgcgtgg tggttgattc
     8581 cccgaacgcg cgcttcggca tcatcaccgc cggcaaggcc tatctcgacg tgcgccaggc
     8641 gctcgccgat ctcgggctcg acgatgaagc ctgcgcgcgc gtcggcatcc gcgtgttcaa
     8701 ggtcggctgc gtgtggccgt tggacgcgca ggacgcgcgc gccttcgccg aaggcctgca
     8761 ggaaatcctg gtggtcgagg aaaagcgcca gatcctcgaa tacgcgctga aggaagaact
     8821 gtacaactgg cgcgaggacg tgcgaccgcg cgtgttcggc aagttcgacg agcgcggcaa
     8881 cgagggcggc gaatggtcgg tcccgcgcgg caactggctg ctgccggcac gctacgagct
     8941 gtcgccggcg atcatcgcgc gcgcggtcgc caagcgcctg gccggcctcg acctgcccga
     9001 agacatccgc tcacgcattg ccgcccgact cgcggtgctc gacgccgccg aaaccgtcac
     9061 cgctcccaag ccggccgaag aacgcaagcc atggttctgc tcgggctgtc cacacaacac
     9121 gtcgacccgc gtgcccgaag gctcgcgcgc gctggccggc atcggctgcc attacatggc
     9181 gatgtggatg gaccgcaaga ccgagacctt cacgcagatg ggcggcgaag gcgtcagctg
     9241 gctcggccag atgcatttct cgaacgacag gcacgtgttc gtcaatctcg gcgacggcac
     9301 ctatttccat tccggccatc tcgcgattcg cgcggcggtc gccgccggtg ccagcgtcac
     9361 ctacaagatt ctctacaacg atgcggtcgc gatgaccggc gggcaaccgg tcgacggcgt
     9421 gctgacggtg ccgcagatcg cgcagcagct gattgccgaa ggcgtgaaga agctggtgat
     9481 cgtcaccgac gagccggaaa agtacacgcc gcaggtgaag ctgcccgagg gcgtgcccgt
     9541 gttccaccgc gacgaactcg accgcgtgca gatcgaactg cgcgagacgc ccggcaccac
     9601 ggtgctgatc tacgaccaga cctgcgcgac cgagaagcgg cgccgccgca agcgcggcac
     9661 ctatcccgac ccggcacgcc gcctgttcat caacgatgcg gtgtgcgaag gctgcggcga
     9721 ctgctcggtg cagtcgaact gcctgtcggt cgagccgctg gccacgccgt tcggcaccaa
     9781 gcgccgcatc aaccaatcct cgtgcaacaa ggacttctcc tgcgcgaagg gcttctgccc
     9841 gagcttcgtc gccgccgagg gcgcgcaacc gcgccgcccg caaggcgccg gcaagggtgt
     9901 gtcgcagccg gatttcgcgg cgttgccgct gccgaacctg ccggacctgg cg
//
LOCUS       DATLGF010000130         2035 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_145990, whole genome shotgun sequence.
ACCESSION   DATLGF010000130 DATLGF010000000
VERSION     DATLGF010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145990"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VM406_03900"
     CDS             <1..271
                     /locus_tag="VM406_03900"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVL75135.1"
                     /translation="REGGYLREQLLALSDDEHRCRYKILHIDANWKNYVAEMHLLPVT
                     EGDKCFGEWWAEFDVPAEEEAAAIERVQGTFRTFFECVNQRFGRK"
     gene            268..1533
                     /locus_tag="VM406_03905"
     CDS             268..1533
                     /locus_tag="VM406_03905"
                     /inference="COORDINATES: protein motif:HMM:NF002674.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HVL75136.1"
                     /translation="MSARPPVAWVVGTFDTKAEELNYLAALLREAGVRALTVDVSTGA
                     ARLPEQAGRPDVGAREIARFHPDGEDAVFGSGDRGQAVIAMSAALCALVSARLRDGEI
                     GGMLGIGGSGGTSMIAPAMRLLPLGMPKLLVSTMAAGNMAPFVDVVDMTTMFPVTDIA
                     GLNRLSRRILANAAHALAGMLLHPPPASGDERPAIAISMFGVTTPCVQAIRGLLGDAY
                     DTQVFHANGAGGRTLERLAAGGHFSVVVDITTTEVGQHLAGAVCDAGPDRLHAAAVHG
                     LPWIGSLGALDMLNWGPIETVPERYRGRTFHVHNANVTLMRTNAEELAAAGRVIAARL
                     NEAAGPVWLYVPEGGLSALDTPGKPFHDPAANRVLFDTLSACFEQTATHHLVRLPHHI
                     NDPEFAGIVAARTEQLMRERAPAGSAERA"
     gene            complement(1553..>2035)
                     /locus_tag="VM406_03910"
     CDS             complement(1553..>2035)
                     /locus_tag="VM406_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL75137.1"
                     /translation="GWGSEFVIRLPRAASEDAPPGGDDAGTQERAPASASLRLLVVDD
                     NVDAANTLASLLRSAGHQVSIAYDAREALACAERESPHALLLDIGLPDIDGFELARRL
                     RRLPQTAKATLVAVTGYGRNEDRARSKASGFDHHFVKPVDPRKLARLLDGVAREPGSA
                     "
BASE COUNT          308 a          694 c          716 g          317 t
ORIGIN      
        1 gcgcgagggc ggctatttgc gcgaacagct gctcgcgctg tccgacgacg aacatcgctg
       61 ccgctacaag atcctgcaca tcgacgcgaa ctggaagaac tacgtcgccg agatgcacct
      121 gctgcctgtc accgaaggcg acaagtgctt cggcgagtgg tgggccgaat tcgatgtgcc
      181 ggcggaagaa gaagcggcgg cgatcgagcg cgtgcagggc acgttccgca cgttcttcga
      241 gtgcgtcaat cagcgcttcg gccggaaatg agtgcccggc ctccggttgc gtgggtggtc
      301 ggcacctttg acaccaaggc ggaggagctg aactacctgg ccgcgctgct gcgcgaggcc
      361 ggcgtgcggg cgttgacggt ggatgtgagc accggcgcgg cgcgcctgcc ggagcaggca
      421 gggcggcccg atgtcggtgc gcgtgaaatc gcgcgctttc atcccgacgg cgaggatgcg
      481 gtattcggca gcggcgaccg cggccaggcg gtgatcgcga tgagcgcggc cttgtgcgcg
      541 ctggtgtcgg cacgtctgcg agacggggag atcggcggca tgctgggcat cggcggatcc
      601 ggcggcacct cgatgatcgc gccggcgatg cgcttgcttc cgctggggat gccgaagctc
      661 ttggtgtcca cgatggctgc cggcaacatg gcgcctttcg tcgacgtcgt cgacatgacg
      721 accatgttcc cggtcaccga catcgccggg ctgaaccggc tgtcgcgcag gatccttgcc
      781 aacgccgcgc acgcgctggc cggcatgctg ctgcatccgc cgccggcgtc gggcgacgag
      841 cgtcctgcga tcgcgatttc gatgttcggc gtgaccacac cctgcgtgca ggccatccgc
      901 ggcctcctgg gggacgcata tgacacccag gtattccatg ccaacggcgc aggcggccgc
      961 acgctcgagc ggctcgccgc cggcggccac ttcagcgtcg tcgtcgatat caccacgacc
     1021 gaggttggcc agcatctggc cggcgccgtc tgcgatgccg gccccgacag gctgcatgcg
     1081 gcggccgtgc acggtctgcc atggatcggc tctctcggcg cgctggacat gctcaactgg
     1141 gggccgatcg aaaccgtacc cgagcgctat cgtggtcgta cattccacgt gcacaacgcc
     1201 aacgtgacgc tgatgcgcac caacgcggaa gaactggcgg ccgccggccg cgtcattgcc
     1261 gcgaggctca acgaagcggc gggaccggtg tggctttacg tcccggaagg cgggctgtcc
     1321 gcgctcgaca cgcccggcaa gcccttccat gatccggccg cgaaccgcgt gctgttcgac
     1381 acgctgtctg cctgctttga gcagaccgcc acgcaccacc tcgtgcgcct gccgcatcac
     1441 atcaacgatc cggaattcgc cgggatcgtg gcggcacgga cagagcagct gatgcgcgag
     1501 cgggcgccgg ccggcagcgc cgaacgcgcc tgacggaaga atttccgtcg acctacgcgc
     1561 tccccggctc gcgcgcgacg ccgtccagca gccgcgccag cttccgcgga tcgaccggct
     1621 tgacgaagtg atggtcgaat cccgacgcct tgctgcgcgc ccggtcctcg ttgcggccgt
     1681 agccggtcac cgcaaccagc gtggccttcg cggtctgcgg caggcgtcgc aggcggcgcg
     1741 ccagttcgaa gccgtcgatg tcgggcaggc cgatgtccag cagcagcgcg tgcggcgact
     1801 cgcgttcggc acaggcgagc gcttcgcgcg catcgtacgc gatgctgacc tgatggccgg
     1861 cgctgcgcag cagagaggca agcgtattgg ccgcgtcgac gttgtcgtcc accacaagca
     1921 gccgcagcga cgcactggcc ggcgcgcgtt cctgggtgcc ggcgtcgtcg ccgccgggcg
     1981 gggcgtcctc gctggcggca cgcggcaggc ggatcacgaa ctcgctgccc cagcc
//
LOCUS       DATLGF010000131        15216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_84343,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000131 DATLGF010000000
VERSION     DATLGF010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 15216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15216
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84343"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..2241
                     /locus_tag="VM406_03915"
     CDS             82..2241
                     /locus_tag="VM406_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HVL75138.1"
                     /translation="MTRLPASIPLRPLIRVLAAALAAFSVPGVASAQPPEQEAPTVVS
                     AERMTGRPDIEVILEGDVEIERGPTTVFADHATYDIVEDEVHAIGNIRLLRMGDTYTG
                     DELRMRIAAGRGFVSQPTYSLQRNQGHGTASRIVFDSRERATVYEGTYSTCEGPDPDW
                     YLRSSRLALDQERDEGVGFGTWLYFKNVPIMALPVMSFPLSEERRSGVLPPSIGTTNR
                     GGVELTVPYYFNIAPNRDLTLYPKVYGRRGLQLGVEARYLEPDFHGETRIEGLPNDRV
                     TGTSRYALTSTHSQRFGSRWSFGWNLNHASDDDYPSDFARTQTAASQRLLLRDTHLAY
                     GGNGWGATLRASGYQVLQDPLAPIARPYDRLPQLNLYAGRSNIGGYDWHADSELTRFW
                     HPDQVRGERLVFNPRISYPFIRPGYFLTPRLALHASAYRVGLPGVVDNVSLTRVLPTA
                     SLDGGLIFERDAQFFGEAMTQTLEPRLFYVYTPYRDQSAFPLFDTAEADVSFAQLFSE
                     NRFVGHDRIADANHITAAVVNRLLDTEGGERLRVAVGQRYYLTPQRVALGAVPPPGRS
                     DILAAVYGRLTQTVTVEANVQYSQLQGRVARSNYGVRYQPGPLRVLNLQYRQDRANNL
                     EQIDLSAQWPIASRWYGVGRINYSLPDQRLAETLAGFEYKHDCWIFRFVAQRTPTATR
                     EATTSFFLQLELIGLTRLGSNPIEALRTNIPGYQLVN"
     gene            2266..3639
                     /locus_tag="VM406_03920"
     CDS             2266..3639
                     /locus_tag="VM406_03920"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HVL75139.1"
                     /translation="MNITPISRLVRFAPHSIAVAMAVLCAGTLVALPAAAQASGAPRV
                     QLADAIVAVVNNEAITRVELEQRMRRVERTMQAQGIGLPPVDQFERQVLESMILDRAQ
                     LQLAREMGIRVDDAMLDRAISRIAEQNGMSIAAFRTQLEREGLSFARFREEIREEIAM
                     QRVREREVDNKIQIPESEVDNFLAAEAQAVQAEQEYSISHILVRIPENASAEQIAERQ
                     QRAEDVMRQVQGGSDFARLAATYSDAPDALSGGDLGWRTRDRLPQIFLDAVDPLQPGQ
                     VSRPLRSANGFHILKLNERRSAQEGDALSGAVTQTRARHILIRVNQVTSAAEARRRLA
                     ELKQRLDNGAATFEELARTFSGDGSAAKGGDLGWLYPGDTVPEFERAMNALEPGQISE
                     PVESPFGFHLIQVVERKQDDVSEGRKRGMARLIIRDRKLEEATADWLRQLRDRTYVEY
                     RLDESRY"
     gene            3647..4669
                     /gene="pdxA"
                     /locus_tag="VM406_03925"
     CDS             3647..4669
                     /gene="pdxA"
                     /locus_tag="VM406_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HVL75140.1"
                     /translation="MGPVIAITCGEPAGIGPEISLRAAWALRGEVDCALIGDAAYLAS
                     LAPQIDPAIALMALPIQALRDGGIPAVAGRLPVIDCPVDDPVVPGKPDARNAKAVLQT
                     LDAAIDGIRRGRLAAMVTAPLQKSTINDAGISFSGHTEYLAQKTGTPQVVMMLAGGTP
                     LLRVALATTHLPLKDVPAAITFDGLSTTLDIIDSDLRAKFGIAAPRILVTGLNPHAGE
                     GGYLGREEIDVIAPAIEAARQRGILATGPWPADTLFQQKYLADADCVLAMYHDQGLPV
                     LKHASFGRGVNITLGLPLIRTSVDHGTALDLASQGLGRADVGSMIEAIRLAANMARAS
                     ARMNTA"
     gene            4666..5442
                     /gene="rsmA"
                     /locus_tag="VM406_03930"
     CDS             4666..5442
                     /gene="rsmA"
                     /locus_tag="VM406_03930"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HVL75141.1"
                     /translation="MKHIPRKRFGQNFLTDETVLHDIIRAIDPKPDDVMVEIGPGLAA
                     MTRLLLESLNRLHVVELDRDLVARLKKGFDPARLIVHEGDALQFDFAAIPRPEGSPLR
                     VVGNLPYNISSPLLFHLAQIAPIVHDQHFMLQKEVVERMVAEPGGKSYGRLSVMLQWR
                     YYMEMLFVVPPTAFDPPPQVDSAIVRMIPLAQPLECDAALLEQVVAKAFSQRRKVIRN
                     CLAGMFSEQQLVDAGVDPQVRPEAVPVERYVALANLLRAK"
     gene            complement(5692..6081)
                     /gene="gloA"
                     /locus_tag="VM406_03935"
     CDS             complement(5692..6081)
                     /gene="gloA"
                     /locus_tag="VM406_03935"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HVL75142.1"
                     /translation="MRILHTMLRVGDLQRSIDFYTKVLGMKLLRTTDRPEQKYSLAFL
                     GYGSNPEHAEIELTYNYGVDSYDLGSAYGHIAIGVEDAYKMCEQVKAAGGNVTREAGP
                     VKGGSTVIAFVQDPDGYKIELIERKNG"
     gene            complement(6092..6952)
                     /locus_tag="VM406_03940"
     CDS             complement(6092..6952)
                     /locus_tag="VM406_03940"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HVL75143.1"
                     /translation="MKLRRPTPPDPRQLALQLDFSDPPAPAPSRTDPPAPPASAPLPP
                     AHPRRRLLLRDHPLDYRLLRSKRRTIGFLIDDEGLRITAPKWVTLAEIENAIREKERW
                     ILNKLSERRERLARRLPAQMQWCDGAALPYLGIEITLRVRAAQTAGVAFDAAGRELTV
                     NLPPDSGEQQLKDRVQAWLQQEARRVFAERLPLYAEKLGASYRAFALSSATTQWGSCT
                     ADGRIRLNWRLIHFGLSIIDYVVAHELAHLREMNHSPRFWATVQSIFPGFEEARRALR
                     ERGPETLPIF"
     gene            complement(6924..7709)
                     /locus_tag="VM406_03945"
     CDS             complement(6924..7709)
                     /locus_tag="VM406_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HVL75144.1"
                     /translation="MSKPILFLRSLAFALLMLVATVVWAPLCFLFAPLPYKQRYHMTA
                     IWNRIIIWGAKAICGIHYQVKGMENLPDAPVILLSKHQSAWETIFYLLVMPRPLVYVF
                     KKELLYIPFFGWGIALLRMIPIDRSKGKDAFAQVVIHGRKRLADGQWVIMFPEGTRSA
                     VGSKGKYKAGGTRLAVETNTPVVPIAMNSGECWPKNSFIKRPGTITVSIGKPIAPDGM
                     DAVQLLEKVENWIESEMRVISSPGIYSNAPYPHSIEAATPDSA"
     gene            complement(7735..8295)
                     /gene="gmhB"
                     /locus_tag="VM406_03950"
     CDS             complement(7735..8295)
                     /gene="gmhB"
                     /locus_tag="VM406_03950"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HVL75145.1"
                     /translation="MKLVILDRDGVINHDSDHFIKSPDEWVPIPGSLQAIARLNQAGY
                     KVVVCTNQSGVARGLFDMAALNAIHQKMHASARQAGADISAVFFCPHAADDNCECRKP
                     KPGMLQAISKRFDVSLKGVASVGDSLRDLQSSFLVGCTPYLVLTGKGERTREKGGLPP
                     GTAVYPDLAAVVDHLLKVDAPVDATV"
     gene            complement(8297..10393)
                     /gene="glyS"
                     /locus_tag="VM406_03955"
     CDS             complement(8297..10393)
                     /gene="glyS"
                     /locus_tag="VM406_03955"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HVL75146.1"
                     /translation="MTQTLLVELLTEELPPKALAKLGEAFAAGIFNVLKTRDFVEADA
                     VATSFATPRRLAVSITGVRAVSPDKSIREKILPVAVALDKEGKPAAPLAKKLAALGFP
                     DLKVSDLERASDGKAESFFHTYTAPGAPLHEGLQAALEDTLAKLPIPKVMSYQRPDGA
                     TVHFVRPAHKLVALHGAEVVPVAILGLAAGRITLGHRFLSSGDIAIDHAQDYASTLEV
                     RGKVIASQPARKEKIRAALHDRAGQDKVLMPEALLDEVTALVEWPVVYECKFEDSFLA
                     VPQECLILTMQTNQKYFALTDANGRLRSRFLIVSNLETVDPQHIIGGNERVVRPRLAD
                     AKFFFEQDQKKRLEDRVPGLANVVYHNKLGTQAQRTERVKALAGAIAAAMGAAMDANV
                     ALAERAALLAKADLLTDMVGEFPELQGIMGTYYARHDGEHEDVAQAISEHYQPRFAGD
                     ALPATGTGTAVALADKLETLVGIWGIGLQPTGDKDPFALRRHALGVLRMLVEKRLPLS
                     LSKLLDQAAQQFAGIPAFKDAREEVTAFTYERLRGLLREHGYAQGEIEAVIAQQPDRL
                     DNLLERLEAVRSFAALPEAASLAAANKRITNILKKTEAAGTAVNPGLLKELAEEGLYS
                     AMNLLKPQVDAAFAGGDFTGTLKALAQLRTNVDAFFNDVMVMVDDERLRNNRIALLSD
                     LHGMMNRVADISKLAA"
     gene            complement(10471..11382)
                     /gene="glyQ"
                     /locus_tag="VM406_03960"
     CDS             complement(10471..11382)
                     /gene="glyQ"
                     /locus_tag="VM406_03960"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HVL75147.1"
                     /translation="MLTFQQVILKLQAYWDAQGCALLQPYDMEVGAGTSHTATFLRAI
                     GPEPWRAAYVQPSRRPKDGRYGENPNRMQHYYQYQVVLKPAPENILDLYLGSLEALGF
                     DLKQNDVRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCKPVLGEITY
                     GLERLAMYLQGVENVYDLVWTEWEEHGVTKRLTYGDVFHQNEVEQSTFNFEHSNTEFL
                     FSLFSNYETEAKRLMEVPLALPAYEMILKAAHTFNLLDARGAISVTERAAYIGRIRNL
                     SRSVAQAYYESRERLGFPMCGDVRQAA"
     gene            complement(11482..13032)
                     /gene="lnt"
                     /locus_tag="VM406_03965"
     CDS             complement(11482..13032)
                     /gene="lnt"
                     /locus_tag="VM406_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HVL75148.1"
                     /translation="MRTRPFPISSSLALPLVALLAGAVNVFAFAPFGLWPLQIATLAL
                     AFHLVLAASNARAATLIGWAYGTGWLVTGVHWLYISMNRYGGLPGWMAALAVLALAAG
                     LSLYAGIALGGARWLADRWRFSRPVMLLLALPAAWTLAEWSRGWILTGFPWVSSGYAH
                     SVGPLAGYAPVVGVYGIGWVAAFIAGALLLLPRLRWPAAIAVLLLGGGFALKNVQWTV
                     PHGEPITVRLLQGNVPQDLKFEPRVIDATLTLYNAMITGTPADLIATPETALPMLSSR
                     LPPGYLDNLADFAERTDSHIAVGLPVVLGPGEYANSVLGLGPGAHAEPYRYDKHHLVP
                     FGEFIPLGARWFVNMMEIPLGDFARGRLLQHPFQVRDQWVMPNVCYEDLFGEEIAAQL
                     RAGVRGEMPQATMLLNVSNIAWFGDSIAVPQHLQVSQMRSLETGRPMLRATNTGATAV
                     VGPDGKVQAQLPPFSLGALSAEVQGYAGTTPYILLGNVPVVLLAFLLLAAACLSGRNP
                     RPAAGKNS"
     gene            complement(13071..13961)
                     /locus_tag="VM406_03970"
     CDS             complement(13071..13961)
                     /locus_tag="VM406_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HVL75149.1"
                     /translation="MHDQPGGARFTDTKPQRSLFERLTALISPEPENRAELLTILQDA
                     QQRGLIDADALSMIEGVFQVSDLSARDIMVPRPQMDVIDITKPIESWIPMVLETAHSR
                     FPAVEGDRDKVAGILLAKDLLRYYAEELFDVRDMLRPAVFIPESKQLNVLLRDFRANR
                     NHMAIVVDEYGGVAGLITIEDVLEQIVGDIEDEYDFDEEEDNILPVHDGARGLRWRVK
                     ALTEITQFNEALSTDFSDEDVDTIGGLVANHLGRVPRKGDVFDIGNLRFEVLRADARQ
                     VHVLLVEKLPMPVAVAQDDH"
     gene            complement(14123..14584)
                     /gene="ybeY"
                     /locus_tag="VM406_03975"
     CDS             complement(14123..14584)
                     /gene="ybeY"
                     /locus_tag="VM406_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HVL75150.1"
                     /translation="MTKKHKLALSVQYADARLKDVVTRDKLRRWVQAALFAPAELTIR
                     FVNAEEGRTLNREYRGKDYATNVLTFAYTEDEDAELTHADIILCTDVLEREAAEQRKP
                     LLAHAAHLVVHGVLHAQGYDHETDEEAVEMEGLEVEILASLGEANPYLPGE"
     gene            complement(14568..>15216)
                     /locus_tag="VM406_03980"
     CDS             complement(14568..>15216)
                     /locus_tag="VM406_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HVL75151.1"
                     /translation="TPNQTEYLRSILEHDVSFGVGPAGTGKTYLAVACAVDALERDAI
                     KRIVLTRPAVEAGERLGFLPGDLAQKVDPYLRPLYDALYDLLGFDRTQKMFEKQVIEI
                     APLAYMRGRTLNHAFIILDEAQNTTPEQMKMFLTRIGFGSKAVVTGDITQIDLQRTQK
                     SGLVDAMQILKKVRGIAFTHFTSADVVRHPLVARIVDAYDAASTPKRTARNDEKA"
BASE COUNT         2595 a         4935 c         5031 g         2655 t
ORIGIN      
        1 gacatgctga agcttgagga ggatgagttc ccatataata agggattaag ctcgaaaaag
       61 cgtccgccga ccccgccctt catgacccgg ttgccagcgt caattcccct gcgtcccctg
      121 attcgggttc tcgccgccgc gctggcggcg ttttcggttc caggcgttgc gtcggcgcag
      181 ccgcccgagc aggaggcgcc caccgtcgtc agcgccgaac gcatgaccgg ccggccggac
      241 atcgaagtca tcctcgaagg cgacgtcgag atcgaacgcg gcccgaccac ggtgttcgcg
      301 gaccatgcca cctacgacat cgtcgaggac gaagtccatg cgatcggcaa catccggctg
      361 ctgcgcatgg gtgataccta taccggcgac gaactgcgca tgcgcatcgc cgccgggcgc
      421 ggtttcgtgt cccagcccac ctattcgctg cagcgcaacc aggggcatgg aacggcgtcg
      481 cgcatcgtgt tcgacagccg cgagcgcgcc accgtgtacg aaggtaccta cagtacctgc
      541 gaaggcccgg acccggactg gtacctgagg tcgagccggc ttgcgctgga ccaggagcgc
      601 gacgagggtg tcggtttcgg cacctggctc tatttcaaga atgtgccgat catggcgctg
      661 ccggtgatgt cgttcccgct ctcggaagaa cgtagaagcg gcgtgctgcc gccgagcatc
      721 ggcaccacca accgcggcgg cgtcgagctg accgtcccgt actacttcaa cattgcaccg
      781 aaccgcgacc tgacgctcta tcccaaggtc tatgggcggc gcgggctgca gctcggcgtc
      841 gaggcgcgct acctcgagcc ggattttcat ggcgagacgc gcatcgaagg cttgccgaac
      901 gaccgcgtca ccggcaccag ccgctacgcg ctcacctcga cccactcgca gcgcttcggc
      961 tcgcgctggt cgttcggctg gaatctgaat cacgcctccg acgacgatta cccgagcgat
     1021 tttgcgcgca cacagactgc ggcctcgcag cgcctgctgc tgcgcgacac gcatctcgcc
     1081 tatggcggca atggctgggg cgcgacgctg cgcgcctccg gctaccaggt tctgcaggat
     1141 ccgctcgcgc cgatcgcacg gccctatgac cggctgcccc agctcaacct gtacgcgggc
     1201 cgcagcaata tcggcggtta cgattggcac gcggattcgg agctgacgcg gttctggcat
     1261 ccggaccagg tacgcggcga gcgcctggta ttcaatccgc gaatttccta tccgttcatc
     1321 cgccccggct atttcctcac gccgcgtctt gctctgcacg cctccgccta tcgcgtcggc
     1381 ctgccgggcg tcgtcgacaa cgtctcgctc acccgtgtgc tgccgaccgc ttcgctggac
     1441 ggcggcctga ttttcgagcg cgatgcccag ttcttcggcg aggcgatgac gcagacgctg
     1501 gagccgcgcc tgttctacgt ctacacccct taccgcgacc aaagtgcgtt cccgttgttc
     1561 gacactgcgg aggcggacgt cagcttcgcc cagctgttca gcgaaaaccg cttcgtcggt
     1621 cacgaccgca ttgccgatgc gaaccacatc accgccgccg tggtgaaccg cctgctggac
     1681 accgaaggcg gggagcggct gcgcgttgcg gtcggccagc gctattacct gacgccccaa
     1741 cgcgtggcgc tcggcgcggt gcccccaccc gggcgttccg acattctggc cgccgtgtac
     1801 ggccggctga cgcagaccgt caccgtcgag gctaatgtcc agtacagcca gctgcaggga
     1861 cgtgtcgcac gctccaacta cggcgtgcgc taccagcccg gtccgctgcg cgtgctcaac
     1921 ctgcagtacc gccaggaccg cgctaacaat ctggagcaga tcgacctctc ggcccagtgg
     1981 ccgatcgcgt cgcgctggta tggcgtgggc cgcatcaact actcgctgcc cgaccaacgg
     2041 cttgcggaaa cgctcgccgg gttcgagtac aagcatgact gctggatctt ccgcttcgtc
     2101 gcgcaacgca cgccaaccgc aacccgcgag gcgaccacat ccttcttcct gcaactggaa
     2161 ctgatcgggc taacccgcct cggttccaat ccgatcgaag cgctccgcac caacattccc
     2221 ggctatcagc tggtgaattg atccgtaact tctgacaggc ccaagatgaa catcactccg
     2281 atttcccgcc tcgtacgttt cgcgccgcat tcgatcgcag tcgcgatggc ggtgctgtgc
     2341 gccggcacgc tggtcgcgct tccggccgcc gcgcaagcgt ccggtgcgcc gcgcgtgcaa
     2401 ttggccgatg cgatcgtggc cgtggtgaac aatgaagcga tcacccgtgt cgaactcgag
     2461 caacgcatgc gccgcgtgga gcgcacgatg caggcccagg gcatcggcct gccgccggtc
     2521 gatcagttcg agcgccaggt gctcgagagc atgatcctcg accgcgcgca gctccagctc
     2581 gcacgcgaga tgggcattcg cgtcgatgat gcgatgctcg accgagccat ttcgcgcatc
     2641 gccgaacaga acggcatgag catcgcggcg ttccgtaccc agctcgaacg cgaagggctg
     2701 tcgttcgccc gcttccgcga ggagatccgc gaggagatcg cgatgcaacg cgtgcgcgag
     2761 cgcgaggtcg acaacaagat ccagatcccg gaatcggaag tcgacaattt cctcgcggcc
     2821 gaagcccagg cggtgcaggc cgaacaggag tacagcatca gccacatcct ggtgcgcatt
     2881 cccgaaaatg ccagcgccga gcagatcgcc gagcgccagc agcgcgccga ggacgtgatg
     2941 cgccaggtgc agggcgggag tgatttcgct cgccttgccg cgacgtattc ggatgcgccc
     3001 gacgcgctgt caggcggcga tcttggctgg cgcacacgcg accgcctgcc gcagattttc
     3061 ctcgacgccg tcgacccgct ccagcccggg caggtgtcgc ggccgctgcg cagcgccaac
     3121 ggtttccaca tcctcaagct caatgagcgg cgcagcgcgc aggaaggcga tgcgctcagc
     3181 ggagccgtta cccagacgcg cgcgcgccac atcctgatcc gcgtgaacca ggtcacttcg
     3241 gccgccgagg cgcgccgcag gctggcggaa ctcaagcagc gcctggacaa cggcgccgcc
     3301 accttcgagg aactggcccg caccttctcc ggcgacggca gcgcggccaa gggcggcgac
     3361 ctcggctggc tgtatccggg cgacacggtg ccggagttcg agcgtgcgat gaatgcgctg
     3421 gaaccggggc agatcagcga gccggtcgag tcgccgttcg gcttccacct gatccaggtg
     3481 gtcgaacgca agcaggatga cgtgtccgaa gggcgcaagc gcggcatggc gcgcctgatc
     3541 atccgcgacc gcaagctgga ggaagcgacc gcggactggc tgcgccagtt gcgcgatcgc
     3601 acctatgtcg aataccgcct cgacgaatcg cgctactgat ccggccatgg gccccgtcat
     3661 cgcaatcacc tgcggcgagc cggccggcat cggcccggag atctcgctgc gggccgcgtg
     3721 ggcactgcgc ggcgaggtcg actgcgcgct gatcggcgat gccgcctacc tggcgtcgct
     3781 ggccccgcag atcgatccgg ccatcgcctt gatggcgctc ccgatccagg cgctgcgcga
     3841 tggcggcatt ccggcggtgg caggacgcct gcccgtcatc gactgtccgg tggacgaccc
     3901 cgtcgttccc ggcaagcccg atgcacgcaa tgcgaaggct gtgctgcaga cgctggatgc
     3961 ggcgatcgac ggcatccgac gcggccgtct tgcggcgatg gtcaccgcac cgctgcagaa
     4021 aagcacgatc aacgatgccg gcatttcttt ctccggccat acggaatacc ttgcacagaa
     4081 gaccggaacg ccgcaggtgg taatgatgct cgccggcggc acgccgctgc tgcgagtcgc
     4141 gctggcgacc acgcacctgc cgctgaagga cgttccggcg gcgatcacct tcgatgggct
     4201 gtcgaccacg ctggacatca tcgacagcga cctgcgcgcg aaattcggca tcgccgcgcc
     4261 gcgcatcctg gtgaccgggc tcaatccgca cgcgggtgag ggcggctatc tgggccgcga
     4321 ggaaatcgac gtgatcgccc cggcgatcga agcagcccgg cagcgcggca tcctggcgac
     4381 cggcccgtgg ccggcagaca cgctgttcca gcagaagtac ctggccgatg ccgactgcgt
     4441 gctcgcgatg taccacgacc agggcttgcc ggtcctgaag catgcgagct tcggccgcgg
     4501 cgtgaacatc accctcggtc ttccgctgat ccgtacttcc gtcgaccatg ggacggcgct
     4561 ggatcttgcg tcgcagggac tgggacgagc cgatgtcggc agcatgatcg aggcgatccg
     4621 cctggcggcg aacatggcgc gcgcatccgc gcgtatgaac acagcatgaa gcacatcccg
     4681 cgcaaacgct tcggccagaa cttcctgacc gatgagactg tcctgcacga catcatccgc
     4741 gcaatcgatc cgaagcccga cgacgtgatg gtcgagatcg gcccgggcct ggccgcgatg
     4801 acgcggctgc tgctcgaatc gttgaaccgg ctgcatgtgg tcgaactgga ccgcgacctg
     4861 gtcgcgcgcc tgaagaaagg attcgatccg gcacgcctga tcgtccacga gggcgacgcg
     4921 ctgcagttcg attttgcagc gatcccgcgt ccggaaggtt cgcctctgcg cgtggtcggc
     4981 aacctgcctt acaacatttc cagcccgctg ctgtttcacc tcgcgcagat cgcgccgatc
     5041 gtccacgacc agcatttcat gctgcagaag gaagtggtcg agcgcatggt ggcggagccc
     5101 ggcggcaagt cctacgggcg cctgtcggtg atgctgcaat ggcgctacta catggagatg
     5161 ctgtttgtgg tgccgccgac tgccttcgat ccgccgccgc aggtcgattc ggcgatcgtg
     5221 cgcatgattc cgctcgcgca gccgctggag tgcgacgcgg cattgctgga gcaggtggtg
     5281 gcgaaagcgt tctcgcagcg gcgcaaggtc atccggaatt gcctggccgg gatgttcagc
     5341 gagcagcagc tcgtcgacgc cggcgtggac ccgcaggtca ggccggaggc ggtgccggtc
     5401 gagcggtatg tggccctggc gaatctgctt cgggcgaagt gagcgtcgga cggcgtgcgg
     5461 cgggatgaga tccttcgcct gcggctcagg atgacaggtt cggggaggac ttgtcgtcct
     5521 gagcgccagc gaaggacctc atccattcgc acagcgacga ccctgtttca agcctgatga
     5581 gatccctcgc ctgcggctca ggatgacagg ttcggggagg acctcatccg ttcgcacagc
     5641 gacgatgccg ttacggtccg agatgagatc ctcggcctcc cgtaaccacg cctacccgtt
     5701 cttccgctcg atcaattcga tcttgtagcc gtccgggtcc tgcacgaacg caatcaccgt
     5761 gcttccgcct ttcaccggcc cggcttcgcg ggtgacgttg ccgcctgcgg ccttgacctg
     5821 ttcgcacatc ttgtacgcat cctcgacgcc gatcgcgatg tgaccatatg cgctgccaag
     5881 atcgtaggaa tccacgccgt agttgtaggt cagctcgatt tccgcatgtt cgggattgct
     5941 gccgtagccg aggaaagcga ggctgtactt ctgttccggg cggtcggtcg tgcgcagcag
     6001 cttcatgccg agcaccttgg tgtagaagtc gatggagcgc tgcaggtcgc cgacccgcag
     6061 catcgtgtgc aggatgcgca tggtaatggt cctagaaaat gggcagggtt tccggcccgc
     6121 gttcacgcaa cgcacggcgc gcttcctcga agccgggaaa gatcgattgt actgtcgccc
     6181 agaaacgcgg gctgtgattc atctcgcgca agtgcgcgag ttcatgggcc accacgtaat
     6241 cgatgatgga aagcccgaaa tggatcagcc gccaattcag gcggatgcgc ccgtccgcgg
     6301 tacacgagcc ccattgcgtg gtggccgacg acagtgcgaa cgcgcggtag ctcgcgccca
     6361 gtttttccgc atacagcggc agacgctcgg cgaacacgcg cctggcctcc tgctgcagcc
     6421 acgcctgcac ccggtccttc agctgctgct cgcccgaatc cggcggcagg ttcaccgtca
     6481 gctcgcggcc ggccgcgtcg aaggccacgc cggccgtctg ggccgcgcgc acgcgcaatg
     6541 tgatttcgat gccgagataa ggcagggccg cgccgtcgca ccactgcatc tgtgccggca
     6601 ggcggcgcgc gaggcgttcg cgccgttcgc tgagcttgtt caggatccag cgctccttct
     6661 cgcgaatcgc gttttcgatt tcggcaagcg tcacccactt cggcgcggtg atgcgcaagc
     6721 cttcatcgtc gatcaggaag ccgatcgtgc gccgcttcga acgcagcagc cggtagtcga
     6781 gcggatgatc gcgcaacagc aggcggcggc gcggatgtgc aggaggcaat ggggcagacg
     6841 ctggcggagc cggcggatcg gtccgggatg gagcgggggc cggcggatcg gaaaaatcga
     6901 gctgcagcgc cagttggcgc ggatcaggcg gagtcgggcg tcgcagcttc aatggaatgc
     6961 ggataaggcg cgttggaata gatgccgggc gaggagatca cccgcatctc ggattctatc
     7021 cagttctcca ctttctcgag caactggacc gcatccatgc catcgggcgc aatcggcttg
     7081 ccgatcgata ccgtgatggt gccggggcgc ttgatgaagg aattctttgg ccagcattcg
     7141 ccggagttca tcgcgatcgg caccacgggc gtgttggtct cgacggccag ccgggtgccg
     7201 ccggccttgt acttgccctt gctgccgacc gccgagcgcg tgccctcggg gaacatgatg
     7261 acccactgcc cgtcggccag gcgcttgcgg ccgtgaatga cgacctgcgc gaacgcatcc
     7321 ttgcccttgc tgcggtcgat cgggatcatc cgcaacagcg cgatacccca gccgaagaac
     7381 gggatgtaga gcagttcctt cttgaacacg tagaccagcg ggcgcggcat gaccagcagg
     7441 tagaagatcg tttcccacgc cgactggtgc ttggagagca ggatcaccgg cgcgtccggc
     7501 agattctcca tcccctttac ctggtaatgg atgccgcaga tcgcttttgc tccccagatg
     7561 atgatgcgat tccagatcgc ggtcatgtgg tagcgctgct tgtacggcag cggggcgaac
     7621 aggaagcaca gcggcgccca tacgacggtc gcgaccagca tcagcagggc aaaggcgagc
     7681 gagcgcagga acaggatcgg cttggacatc gggtctccgg gaagagggag gaaattacac
     7741 ggtggcatcg accggggcat ccaccttcag caggtggtcg accacggcgg ccagatcggg
     7801 gtagacggcg gtgccgggcg gcaggccgcc tttttcgcgg gtcctttcgc ccttgccggt
     7861 cagcaccagg tagggggtac agccgaccag gaagctcgac tgcagatcgc gcagcgagtc
     7921 gccgaccgag gccacgccct tgaggctcac gtcgaagcgc ttggaaattg cctgcaacat
     7981 gcccggcttc ggcttgcggc attcgcaatt gtcgtccgcc gcgtgcgggc agaagaacac
     8041 ggccgagata tccgcgccgg cctggcgcgc gctggcgtgc atcttctggt gaatcgcatt
     8101 aagcgcggcc atgtcgaaca ggccgcgcgc gacgccggat tgattggtgc agacgacaac
     8161 tttgtagccc gcctggttca ggcgcgcgat cgcctgcagc gatccgggga tcggcaccca
     8221 ttcgtccgga gacttgatga aatgatccga gtcgtgattg atcacgccat cacggtcgag
     8281 gatgaccagc ttcatgtcat gcggcgagtt tggaaatatc ggccacgcgg ttcatcatgc
     8341 cgtgcagatc agacagcagc gcgatgcgat tgttgcgcag acgttcgtcg tcgaccatca
     8401 ccatcacgtc gttgaagaag gcgtcgacgt tggtgcgcaa ctgggcgagc gccttcagcg
     8461 tgccggtgaa atcccccccg gcaaacgcgg cgtcgacttg cggcttcagc aggttcatcg
     8521 ccgagtacag gccttcttcc gccaattcct tcaacaggcc cgggttgacg gccgtgccgg
     8581 ccgcctcggt cttcttcagg atgttggtga tccgcttgtt ggccgcggcc agcgatgcgg
     8641 cttcaggcag ggcggcaaag ctgcgcaccg cctcgagacg ctcgagcagg ttgtcgagtc
     8701 ggtccggttg ctgggcgatc acggcttcga tctcgccctg cgcatagccg tgctcgcgca
     8761 gcaggccgcg caggcgttcg taagtgaaag cagtcacttc ctcgcgcgca tccttgaagg
     8821 cgggaatgcc ggcgaactgt tgcgccgcct gatcgagcag cttggacagg gacaggggca
     8881 ggcgcttttc gaccagcata cgcagcacgc cgagtgcatg gcggcgcagc gcaaacgggt
     8941 ccttgtcgcc ggtcggctgc aggccgatgc cccagatgcc gaccagggtt tccagcttgt
     9001 cggccagcgc caccgctgtg ccggtcccgg tggcgggaag cgcgtcgccg gcaaaccgcg
     9061 gctggtagtg ctcggagatc gcttgcgcca cgtcctcgtg ctcgccatcg tggcgcgcat
     9121 agtaggtgcc catgatgccc tgcagctccg ggaattctcc gaccatgtcg gtcagcaggt
     9181 cggctttcgc cagcagcgcg gcgcgttcgg ccagcgcgac attggcgtcc atcgcggcgc
     9241 ccatcgcggc ggcgatcgcc ccggccaggg ccttcacgcg ctcagtgcgc tgcgcctggg
     9301 tgcccagctt gttgtgatag accacgttcg ccaggcccgg cacccggtct tccagccgct
     9361 tcttctggtc ctgctcgaag aagaacttgg cgtcggccag gcgcgggcgc accacgcgct
     9421 cgttgccgcc gatgatgtgc tgcggatcga cggtctccag attggagacg atcaggaagc
     9481 gcgaacgcag gcggccgttg gcatcggtca gcgcaaagta tttctggttg gtctgcatcg
     9541 tcaggatcag gcattcctgc ggcacggcca ggaagctgtc ctcgaacttg cattcataca
     9601 ccaccggcca ttcgaccagg gcggtcacct cgtctagcag cgcttccggc atcagcacct
     9661 tgtcctggcc ggcgcggtcg tgcaacgcgg cgcggatctt ttccttgcgg gcgggctggc
     9721 tggcgatcac cttgccgcgc acctccagcg tggacgcgta gtcctgggca tggtcgatcg
     9781 cgatgtcgcc ggatgaaagg aaacggtgtc cgagcgtgat gcggccggcc gccaggccca
     9841 ggatggccac cggcaccacc tcggcgccat gcagcgcgac cagcttgtgc gccgggcgca
     9901 cgaagtgcac ggtcgcgccg tccgggcgct ggtagctcat caccttcgga atcggcagct
     9961 ttgccagcgt gtcttcgagc gccgcctgca ggccttcatg cagcggcgcg ccgggggcgg
    10021 tgtaggtatg gaaaaaactt tcggccttgc cgtcggatgc gcgctccagg tcggaaacct
    10081 tcagatcggg aaagccgagc gcggcgagct tcttcgccag cggcgcggcc ggcttgcctt
    10141 ccttgtcgag ggcgacggcc accggcagga tcttttcgcg gatcgacttg tccggcgata
    10201 cggcgcgtac gccggtgatc gagacggcga gccggcgcgg cgtggcgaac gaggtggcga
    10261 cggcgtcggc ttcgacgaag tcgcgcgtct tgaggacgtt gaagatgccg gcggcaaagg
    10321 cctcgccgag cttcgcaagc gccttcggcg gcagttcttc ggtcagcagt tcgactagca
    10381 gggtctgggt catggcttgt cattcccgca agcgggatgg tttgcgttgt tctcgttgga
    10441 gcaggattcc cgtgcgcggg cgcggcggat tcaggcggcc tggcgcacgt ccccgcacat
    10501 cgggaagccg aggcgctcgc gcgattcgta atacgcctgc gcgaccgagc gcgacagatt
    10561 gcggatgcgg ccgatgtagg cggcgcgctc ggtcaccgaa atcgcgccgc gcgcgtccag
    10621 caggttgaag gtgtgcgcgg ccttcaggat catctcgtag gccggcagcg cgagcggcac
    10681 ttccatcagg cgcttggctt cggtttcgta gttcgagaac agcgagaaca ggaattcggt
    10741 gttcgagtgc tcgaaattga aggtcgactg ctcgacttcg ttctggtgga acacgtcgcc
    10801 ataggtcagg cgcttggtca cgccatgctc ttcccattcg gtccatacca ggtcgtagac
    10861 gttttccacg ccctgcaggt acatcgccag gcgctccagg ccgtaggtga tctcgccgag
    10921 caccggcttg cagtcgatgc cgccgacctg ctggaagtag gtgaactgcg tgacttccat
    10981 gccgttgagc cacacttccc agcccaggcc ccaggcgccc agcgtcgggt tttcccaatc
    11041 gtcctcgacg aagcgcacgt cgttctgctt cagatcgaag ccgagtgcct ccagcgagcc
    11101 gagatacaag tcgaggatgt tttccggcgc gggcttgagc accacctggt actggtagta
    11161 atgctgcatc cggttcgggt tctcgccgta gcgcccgtcc ttcggccggc gcgacggctg
    11221 cacgtaggcg gcgcgccacg gttccggccc gatcgcgcgc aggaaggtcg cggtatgcga
    11281 ggtcccggcg ccgacttcca tgtcataggg ttggagcagt gcgcagccct gcgcatccca
    11341 gtaggcctgc agcttgagga tgacttgttg aaatgtgagc atgatcagat gggttcgacg
    11401 cgtggcgccg gccgaagcgg cagcaaggga agcgacaccg gcacgacatc gtccacagta
    11461 tccggacgaa aaccaccgat tttagctgtt tttaccagcg gcaggccggg gattgcgccc
    11521 gctcaggcag gctgccgcca gcagcaggaa ggccagcagc acgaccggca cgttgccgag
    11581 gaggatgtag ggcgtggtgc cggcatagcc ctggacctcg gcgctgagcg cgccgagcga
    11641 aaacggcggg agctgcgcct gcaccttgcc gtcggggccg acgacggcgg tggcgccggt
    11701 gttggtcgcg cgcagcatcg ggcgtccggt ttcgagcgaa cgcatctgcg agacctgcag
    11761 atgttgcggc accgcgatcg aatcgccgaa ccaggcgatg ttcgagacat tgagcagcat
    11821 ggtggcctgc ggcatctcgc cgcggacgcc ggcacgtagc tgggcggcga tttcctcgcc
    11881 gaacaagtcc tcgtagcaga cgttcggcat cacccactgg tcgcggacct ggaacgggtg
    11941 ctgcagcagc ctgccgcggg cgaagtcgcc aaggggaatt tccatcatgt tcacgaacca
    12001 gcgtgcgccg agcgggatga attccccgaa agggacgagg tggtgcttgt cgtagcggta
    12061 aggttcggcg tgcgcgccgg gaccgagccc gagcacgctg ttcgcatact cgccggggcc
    12121 gagcacgacc ggaaggccga ccgcgatgtg gctgtccgtg cgttcggcga agtcggcgag
    12181 gttgtcgaga tagcccggcg gcaggcgcga cgagagcatc ggcagcgcgg tttccggcgt
    12241 ggcgatcaga tcggccggcg tgccggtgat catcgcgttg tacagcgtga gcgtggcgtc
    12301 gattacgcgc ggctcgaact tcaggtcctg cggcacattg ccctggagca ggcgtacggt
    12361 gatcggctcg ccgtggggta ccgtccattg cacgttcttc agcgcgaatc cgccgccaag
    12421 cagcagcacg gcgatcgcgg ctggccagcg cagccgcggc aacagcaaca gcgcgccggc
    12481 gatgaaggcc gcgacccagc cgatgccgta caccccgaca accggggcgt agcccgcaag
    12541 cgggccgacg ctgtgcgcat agcccgaact cacccacggg aagccagtca ggatccagcc
    12601 gcgcgaccat tctgccagcg tccacgcagc cggcagcgcg agcagcagca tgaccggccg
    12661 cgaaaagcgc cagcggtcgg ccagccagcg cgcgccgccc agcgcgatcc cggcgtacag
    12721 gctcagtccc gcggccagcg cgagcaccgc cagcgccgcc atccagccgg gcagtccgcc
    12781 gtagcggttc atgctgatgt aaagccagtg cacgccggtc accagccagc cggtgccgta
    12841 ggcccatccg atcagcgtgg ccgcgcgcgc gttactagca gcaagaacga ggtggaaggc
    12901 tagcgccagc gtggcgatct gcaacggcca caggccgaag ggcgcgaacg cgaagacatt
    12961 taccgcgccc gccagcagcg ccacgagcgg cagcgcgagc gacgaggaaa tcgggaaggg
    13021 gcgggtgcgc acgtattcca tcgctcacgc gcaccgggca aacgcggccg tcaatggtcg
    13081 tcctgcgcga cggccaccgg catcggcagc ttctccacca gcagcacatg cacctggcgc
    13141 gcatcggcgc gcaggacctc gaagcgcagg ttgccgatat cgaacacgtc gcccttgcgc
    13201 ggcacgcggc cgaggtggtt ggcgaccagt ccgcctatgg tgtcgacgtc ctcgtcggaa
    13261 aaatcggtgg acagcgcctc gttgaactgc gtgatttcgg tgagcgcctt cacgcgccag
    13321 cgcaggccgc gcgcgccgtc gtgcaccggc aggatgttgt cctcttcctc gtcgaaatcg
    13381 tattcgtcct cgatgtcacc gacgatctgc tcgagcacgt cctcgatcgt gatcaggccg
    13441 gccacgccgc cgtattcgtc gacgacgatg gccatgtgat tgcggttggc gcggaaatcg
    13501 cgcagcagca cattgagctg cttggattcc ggaatgaaca ccgccggacg cagcatgtcg
    13561 cgcacgtcga aaagttcctc tgcgtaatag cgcagcagat ccttggcaag caggattccg
    13621 gcgaccttgt cgcgatcgcc ctcgacggcc ggaaagcgtg aatgcgcggt ttcgagaacc
    13681 atcggaatcc agctctcgat cggcttggtg atgtcgatca cgtccatctg cggacgcgga
    13741 accatgatgt cgcgggcgga caggtcggaa acctggaaca cgccttcgat catcgacagc
    13801 gcgtccgcat cgatcaggcc gcgctgctgc gcgtcctgca ggatggtgag cagctcggcc
    13861 cggttttccg gctcggggga gatcagtgcg gtcaggcgtt cgaacagcga ccgctgcggt
    13921 ttggtgtcag tgaatctggc accaccaggt tgatcgtgca tttggcggga agccgttgtt
    13981 ggtgtgggca taggatacaa caaatggaat ccgcgaccgc ggggaggttc ccggaaaacg
    14041 aggggccatc gtgcccgcat ggaacgccgc aatggccccg cgggcttcag tgttgccgaa
    14101 agtggaagtt tcccctgcgc tctcactcgc ccggcaagta aggattcgct tcgccgagag
    14161 acgccagaat ctcgacttca agcccttcca tctcgaccgc ttcctcatcg gtttcgtggt
    14221 cgtacccttg ggcgtgcagc acgccgtgaa cgaccaggtg ggcggcgtgg gcgagcagcg
    14281 gcttgcgttg ctcggcggct tcacgttcaa gcacgtcggt gcagaggatg atgtcggcgt
    14341 gggtgagctc cgcgtcctcg tcctcggtat acgcaaaggt cagcacgttg gtcgcgtaat
    14401 ccttgccgcg gtattctcgg ttgagcgtgc ggccttcctc ggcattgacg aaacggatcg
    14461 tcaactcggc cggggcgaac agggcggcct gcacccaccg gcgcagcttg tcgcgcgtga
    14521 cgacatcctt cagccgcgcg tcggcatact gcaccgacag cgcgagctta tgctttttcg
    14581 tcattgcggg cggtgcgctt cggggtgctg gcggcgtcgt aggcatcgac gatgcgcgcc
    14641 accagcgggt ggcgcaccac gtcggcgctg gtgaaatggg tgaaggcgat gccgcgcacc
    14701 ttcttcagga tctgcatcgc gtcgaccagc ccgctctttt gcgtgcgctg caggtcgatc
    14761 tgggtgatgt cgccggtcac caccgccttg ctgccgaaac cgatgcgtgt caggaacatc
    14821 ttcatctgct cgggcgtggt gttctgggct tcatcgagga tgatgaacgc atgattgagc
    14881 gtgcgcccgc gcatgtaggc gagcggggcg atctcgatca cctgtttctc gaacatcttc
    14941 tgcgtgcggt cgaagccgag caggtcgtac agcgcgtcgt acagcgggcg cagataggga
    15001 tcgaccttct gcgcgaggtc gccgggcaga aagccgagcc gctcgccggc ttccaccgcg
    15061 gggcgtgtca gcacgatgcg cttgatcgcg tcgcgctcga gcgcgtcgac cgcgcaggcg
    15121 accgccagat aggtcttgcc ggtgccggcc ggaccgacgc cgaagctcac gtcgtgctcg
    15181 aggatggaac gcaggtactc ggtctggttc ggcgta
//
LOCUS       DATLGF010000132         2056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_52497,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000132 DATLGF010000000
VERSION     DATLGF010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52497"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VM406_03985"
     CDS             complement(<1..786)
                     /locus_tag="VM406_03985"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVL75152.1"
                     /translation="MNIKTSMRSLACALAMIPVLAFAHGGEDHSHDAEAKTNPGKALP
                     VLVGSESPSRLPDGSVFLPKGSQNLLEVRTVLTSLGDVSQTVELKGRVKADPSAGGQV
                     QAPQMGRIVANGVGLPALGQSVKAGQVLAWLEPVVGSVERGNQQAQLAQIRNELAQAE
                     RRVERYAQLEGSIPQKEIDAAVSELQALKQQKAALDGGLSARQPLVAPASGVLSNVAV
                     VAGQVVDAKETIFTVVDPQRLIVEALAYDARLANNIASVSGTTE"
     gene            complement(809..1360)
                     /locus_tag="VM406_03990"
     CDS             complement(809..1360)
                     /locus_tag="VM406_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75153.1"
                     /translation="MKLRTSFLHSALLSAAVTLAATHAPAAFGHAGEDHGDAPAVAVS
                     GQVLAPRTEARSEDVEMLAVYESGALTLYFADYRTNAPIAGAEVEIESGTSKAVAKAI
                     GDGVYKADTPWIGGPGKHPLVVTIQGEQVTDLLEAGLDIAAPAAEIKENRIGKLGKLS
                     VAGSIGGAAALGLVALGLRRRRK"
     gene            complement(1357..>2056)
                     /locus_tag="VM406_03995"
     CDS             complement(1357..>2056)
                     /locus_tag="VM406_03995"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HVL75154.1"
                     /translation="AHADLLLARGETIAVQTELSAAEQALLEVRQDYLQLVGDDRLPA
                     DAVETAPGVQAIDTHPRLLASARRVEAARARAQLTARTGRENPELALLARRERGDADE
                     RYGNAFGVAIRIPLGTAARNAPRMAVAQAELTEAETEHARLREALEQAQRKAQAEMDS
                     ARTRAELAEARLVLARENQALAKKAFSLGEKSLFEFQRVQSALDESALAAGQAAIEQH
                     RAQARLNQASGVLP"
BASE COUNT          263 a          709 c          689 g          395 t
ORIGIN      
        1 ctccgtcgtg cccgacacgc tggcgatgtt gttggccaac cgcgcgtcat aggcgagcgc
       61 ttcgacgatc agccgctgcg ggtcgaccac cgtgaaaatc gtctccttgg cgtcgaccac
      121 ctgtccggcc accacggcca cgttgctgag cacgccggaa gccggcgcca ccagcggctg
      181 gcgcgcggaa agcccgccgt cgagcgccgc cttctgctgc ttcagcgcct gcagctccga
      241 taccgccgcg tcgatttcct tctgcggaat cgagccttca agctgcgcat agcgctcgac
      301 gcggcgctcg gcctgggcga gttcgttgcg gatctgcgcc agttgtgcct gctggttgcc
      361 gcgctcgaca ctgccgacca ccggctccag ccaggccagc acctggccgg ccttcacgct
      421 ctgtccgagc gccgggaggc cgaccccgtt tgccacgatc cggcccatct gcggcgcctg
      481 cacctgcccg ccggccgagg ggtcggcctt gacgcggccc ttcagctcga ccgtctgact
      541 gacgtcgccc aggctggtca gcaccgtgcg cacttcgagc aggttctggc tgcccttggg
      601 caggaatacg ctgccatccg gcaagcgcga aggcgactcg ctgccgacca gaacgggcag
      661 tgccttgccc ggattcgtct tcgcctctgc gtcatggctg tggtcttccc cgccgtgcgc
      721 gaatgcgagc acggggatca tcgcgagggc gcaggccagg ctgcgcatgg aagtcttgat
      781 gttcatgtct gttcctgtct gttccggtct acttcctgcg gcggcgcagg ccgagcgcga
      841 cgaggccgag tgcggccgcg ccgccgatcg agcccgccac gctgagtttt ccgagttttc
      901 cgattcggtt ttctttgatt tcggcggcgg gtgcggcaat gtccaggccg gcttcgagca
      961 ggtccgtcac ttgctcaccc tggatcgtca ccaccaatgg atgcttgccg gggccgccga
     1021 tccagggcgt gtcggccttg tacacgccgt cgccaatggc cttggcgaca gccttcgatg
     1081 tcccgctttc gatttcgact tcggcgccgg cgatcggcgc gttcgtcctg tagtcggcga
     1141 aatagagggt cagtgcgccg ctctcgtaga cggcgagcat ctccacatct tccgagcgcg
     1201 cctcggtgcg tggcgcgagc acctgcccgg acacggcgac ggccggtgca tcgccgtggt
     1261 cttctccggc atggccgaag gcggcgggcg cgtgcgttgc ggcaagcgtc acggcagccg
     1321 acaacagggc ggaatgtaaa aaggatgtgc gaagtttcat ggcaggactc ccgatgcctg
     1381 gttcaggcgc gcttgcgcgc ggtgttgttc gatggccgcc tggccggcgg cgagtgcgga
     1441 ttcgtcgagc gcggactgca cgcgctggaa ctcgaacagg cttttctctc cgagggagaa
     1501 cgccttcttc gccagcgcct ggttttcccg tgccagcaca aggcgcgcct cggccagctc
     1561 ggcgcgcgtc cgtgcgctgt ccatttcggc ctgagccttg cgttgcgcct gttcgagggc
     1621 ttcgcgcagg cgcgcgtgtt ctgtttcggc ttccgtcagc tccgcctggg caacggccat
     1681 gcgcggcgca ttgcgtgcgg cggttccaag tggaatccgg atcgcgacgc cgaaggcgtt
     1741 cccgtagcgc tcgtccgcgt cgccgcgctc gcgtcgcgcg agcagcgcca gctccgggtt
     1801 ctcgcgcccg gttcttgcag tcagttgtgc gcgcgcccgc gccgcttcca cccgccttgc
     1861 gctggcgagc agccgcggat gcgtgtcgat tgcttgcacg ccgggagcgg tctcgactgc
     1921 atcggcaggc aggcggtcgt caccgaccag ttgcaggtag tcctggcgta cctcaagcag
     1981 ggcctgttcg gcggcgctca gttctgtctg caccgcgatg gtctcgccgc gcgccagtag
     2041 cagatcggca tgggcg
//
LOCUS       DATLGF010000133         3413 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_37732,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000133 DATLGF010000000
VERSION     DATLGF010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37732"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..858)
                     /locus_tag="VM406_04000"
     CDS             complement(61..858)
                     /locus_tag="VM406_04000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HVL75155.1"
                     /translation="MRHLRTRGFTLVELVVVIALTGVLASVVAIFLRAPVRQYVDLGR
                     RAELSDTADTALRRIARDLRHALPNSVRVGAGCTGTNVCSIEFFPTVGGGRYRVGSGD
                     ELDFTRADTSFDVVGPMPQASTAHHVVVYNLGIHGADAYSGAAAATDVRRPISGVGAG
                     SLSITSSHPFPFESPGQRFHVVSSPVRYVCNPQAGTLVREAGYGWPEAGTTGTSRLLA
                     RGVSRCLFTYEQSAAAQRSGLVTMQITLSEGGEGVSLYHAVHVSNQP"
     gene            complement(855..1457)
                     /locus_tag="VM406_04005"
     CDS             complement(855..1457)
                     /locus_tag="VM406_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HVL75156.1"
                     /translation="MCTERRRRQAGISLVELVIFIVVVSIGLVGLLAVMNVANRGSAD
                     PLVRKQALAIAESILEEVMLMPFTFCDPDDPAAADAAGASDCGPGMDQNNGGNPFTGP
                     IPASETRYSSTAPFDNVADYAGFTMTSGIADSTGDGTAVIPGLQNYRLQPIGIQRIAL
                     GNITAGGNALLITVTVTDAGGANAVTLQGIRTRYAPRAMP"
     gene            complement(1447..1965)
                     /locus_tag="VM406_04010"
     CDS             complement(1447..1965)
                     /locus_tag="VM406_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75157.1"
                     /translation="MATLRFLNSNAPNHFASMAKRGHVALAGKSGFTLIELAGILVIV
                     AILAVAVTPRFFSQSVFANRAVHDEMVAFLRYAQKSAVAQRRNVCVAFTGSSATLTIS
                     ANAGPAASCSNSLTGPDGQAPYRIDVPAGFASVPAAFHFTALGQASAGQTLSIAGHGV
                     PIRIEAETGYVY"
     gene            complement(2081..2521)
                     /locus_tag="VM406_04015"
     CDS             complement(2081..2521)
                     /locus_tag="VM406_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HVL75158.1"
                     /translation="MKNNQSGFTLIELVVVIVILGILAVTALPRFVDLSGDARTAATQ
                     GVAGALGSASSINYATAVARGTSSGPLASVTTPTSGVIDTSNGCSNAVAQALLQAGVT
                     FGTAAGQYNVTGASLTTVGQTTDCTVRNNDGAQPTATFTLVGAR"
     gene            complement(2558..3085)
                     /locus_tag="VM406_04020"
     CDS             complement(2558..3085)
                     /locus_tag="VM406_04020"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HVL75159.1"
                     /translation="MGHCPTRSRARGFTLLELVVVTSTVALLSGVLLDRVQFYQEMAE
                     RAAMQQTAGAIRTSLYLQALNLIAKGRHNEIDELAKRNPMEALAEKPANYGGEIISVR
                     QNNVVSGHWYFNSDDKTLIYLVHNGKHFRAEHDPKQVVYRVRILRGERAENGSEKPVE
                     GVILEQVNSFSWFKQ"
     gene            complement(3094..>3413)
                     /locus_tag="VM406_04025"
     CDS             complement(3094..>3413)
                     /locus_tag="VM406_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HVL75160.1"
                     /translation="YMTARIEQMRDGVERGESILRTAVATGVFTPVVLQMIAVGEETG
                     ELDELMGDIADMYDREVEYELKTLSAQIEPILITGLAVLVLILALGVFLPIWDLGAAA
                     LNK"
BASE COUNT          619 a         1109 c         1092 g          593 t
ORIGIN      
        1 ccatcaggaa caccgctgac acaagggtga agccgcgctg gcgggcagga cgaagcttca
       61 tcatggctgg ttgctcacgt ggaccgcgtg atagagcgag accccctcgc ccccttccga
      121 caatgtgatc tgcatcgtca ccaggccgga ccgttgcgcc gccgcggact gttcgtaagt
      181 gaagaggcag cggctcacac cacgggcaag cagccggctg gttccggtcg tgccagcctc
      241 gggccagccg tatccggcct cgcgcaccag cgtaccggcc tgcgggttgc agacgtaccg
      301 caccggcgag ctcacgacat gaaagcgctg cccgggcgac tcgaacggaa acgggtggga
      361 ggacgtgatg ctcaggctgc ccgcgccgac gccgctgatc gggcgccgca catccgtcgc
      421 ggcggccgcg ccggaatagg cgtcggctcc gtgtatgccg aggttgtaga cgaccacgtg
      481 atgcgcggtg cttgcctgcg gcatcggccc gaccacgtcg aagctcgtgt cggcgcgtgt
      541 gaaatcgagc tcgtcgccgc ttcccaccct gtaccggccg cctccgaccg tcggaaagaa
      601 ctcgatgctg cacacgttcg tccccgtgca gccggcgccg acgcgcacgc tgttcggaag
      661 cgcatggcgc aggtcgcgcg cgatccggcg caaggccgta tccgccgtat cgctcaactc
      721 ggcgcgacgg ccgagatcga catactggcg caccggcgcg cgcagaaaga tcgcgaccac
      781 ggaggcgagc acgccggtca gcgcaatcac cacgacaagc tcgacgaggg tgaatccccg
      841 ggtgcgcaag tgcctcatgg cattgccctc ggcgcatagc gggtacgaat gccttgcagc
      901 gtgaccgcgt tggcgccgcc ggcatccgtc acggtcacag tgatcagcaa tgcgttgccc
      961 ccggccgtga tattaccgag cgcaatgcgc tgtatgccga tcggctgcag ccggtagttc
     1021 tgcaagccgg gaatgactgc ggtgccgtct ccggtactgt cggcgatgcc gctggtcatc
     1081 gtgaagccgg cgtaatcggc aacgttgtcg aacggcgccg tgctcgagta acgcgtttcc
     1141 gacgccggta tcgggccggt gaacggattg ccgccgttgt tctggtccat gccggggccg
     1201 caatcggagg caccggccgc atcggccgca gccggatcgt ccgggtcgca aaaggtgaac
     1261 ggcatcagca tcacttcctc gaggatcgat tcggcgattg ccagcgcctg cttgcgcacc
     1321 agcggatccg cactgccgcg gttcgccacg ttcatcacgg ccagcaggcc gaccaggccg
     1381 atcgacacca cgacgatgaa aatcaccagt tcgaccaggg aaatgcccgc ctggcggcga
     1441 cggcgctcag tacacatagc cggtttccgc ctcgatcctg atcggcacgc catggccggc
     1501 gatgcttagc gtctgccccg cgctggcctg gccgagcgcg gtgaaatgaa atgcggccgg
     1561 cacactggca aagccggcag ggacatcgat cctgtacgga gcctgcccgt ccggcccggt
     1621 cagggagttg ctgcagcttg cggcagggcc ggcattcgcg gagatggtca gcgtcgccga
     1681 acttccagtg aacgccacgc atacgttgcg gcgctgcgcg accgcgctct tctgcgcata
     1741 gcgcaggaag gccaccattt cgtcatgaac ggcgcggttg gcgaacacgg actgggagaa
     1801 gaagcgtggg gtgaccgcca cggccaagat ggcgacgatg acgaggatgc ctgccagctc
     1861 gatcaaggtg aaacccgact tgcccgcgag tgcaacatgc cctcgcttcg ccatcgaagc
     1921 gaagtggttc ggtgcgttcg agttgagaaa gcggagagtc gccatgagag tgaccgcatg
     1981 gggttgaacc gcttgcagat ctgctgtgcg gccccggaaa gaattcggga ccgcgggcag
     2041 cgactgccgg cggtcccgat tcctttactg tcgccgggtc ttagcgggca ccgaccaggg
     2101 tgaacgttgc ggtcggctga gcaccgtcat tgttacgaac cgtgcagtcg gtggtctggc
     2161 cgacggtggt gagcgaagcg ccggtcacgt tgtattggcc cgctgcggtc ccgaaggtga
     2221 cgccggcctg caggagggct tgagcgacag cgttcgagca gccattgctg gtatcaatca
     2281 cgcccgaggt cggcgtagtc acgctcgcga gcgggccgga agacgtgccg cgcgccacgg
     2341 cggtcgcgta attgatgctg ctcgccgaac ccagcgcacc ggccacgcct tgcgtcgctg
     2401 cagtgcgcgc atcacccgac aggtcgacga aacgcggcag cgcggtcacg gccaggatgc
     2461 cgagaatgac gatgacgacg acgagttcga tcagcgtgaa gccggactga ttgtttttca
     2521 ctttattgct cctcatgttt aaacgccctc agggtgacta ctgcttgaac caagaaaaag
     2581 aattcacctg ctccagaatc acgccctcga ccggcttttc cgagccgttc tccgctcgct
     2641 ctccccgcag aatccggacg cggtagacga cctgtttcgg atcatgttcc gcccggaaat
     2701 gcttgccatt atgcaccagg taaatcagcg ttttatcgtc tgagttgaaa taccagtgcc
     2761 ccgatacaac attgttttgc cgtacggaaa ttatttcgcc gccatagttc gcgggcttct
     2821 ccgcaagcgc ctccatcgga ttgcgcttgg ccagttcatc gatctcgttg tgccgtcctt
     2881 ttgcgatcag attgagcgcc tgcaggtaaa gcgaggtgcg gatcgcaccg gcggtctgct
     2941 gcatggccgc gcgctcagcc atctcctggt agaactgcac gcggtccagc agcacgccgg
     3001 acagcagcgc caccgtgctg gtgaccacca ccaactccag cagcgtgaag ccgcgcgcgc
     3061 ggcttcgagt gggacaatgg cccatgcgtc gcctcacttg ttcagggccg ccgcgcccaa
     3121 atcccagatc ggcaggaaca cgccgagggc gaggatcagg accagcacgg ccaggccggt
     3181 gatgagaatc ggctcgatct gcgccgacag cgtcttcagc tcgtattcga cttcgcggtc
     3241 atacatgtcg gcgatgtcgc ccatgagctc atcgagctcg ccggtttctt cgcccaccgc
     3301 aatcatctgc agcactaccg gcgtgaacac ccctgtggcc accgccgtgc gcaggatcga
     3361 ttcgccgcgc tcgacgccgt cgcgcatctg ctcgatgcgc gcagtcatgt agg
//
LOCUS       DATLGF010000134         8952 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_52524,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000134 DATLGF010000000
VERSION     DATLGF010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 8952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8952
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52524"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VM406_04030"
     CDS             complement(<1..479)
                     /locus_tag="VM406_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HVL75161.1"
                     /translation="MNSLVNIVAQAQADFTAAEDGAALENAKAKYLGKTGQITEQMKG
                     LGKLAPEERKAQGAVINAAKEQIEQALSARREELANAQMQARLNAEAIDVTLPGRGRG
                     VGGIHPVMRTWERIEQIFGSIGFDVADGPEIETDWTNFTALNSPENHPARSMQDTFY"
     gene            complement(622..981)
                     /gene="rplT"
                     /locus_tag="VM406_04035"
     CDS             complement(622..981)
                     /gene="rplT"
                     /locus_tag="VM406_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HVL75162.1"
                     /translation="MPRVKRGVTARARHKKVLDQAKGYRGRRSTVYRIAKQAVMRAGQ
                     YAYRDRRNKKRVFRALWIARINAASRAHGVTYSVFMNGLKKANIGLDRKVLADMAVTD
                     KPAFAAIVNQVKASIAA"
     gene            complement(1003..1200)
                     /gene="rpmI"
                     /locus_tag="VM406_04040"
     CDS             complement(1003..1200)
                     /gene="rpmI"
                     /locus_tag="VM406_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HVL75163.1"
                     /translation="MPKMKTKSSAKKRFRVRPGGTVKRGQAFKRHILTKKTTKNKRQL
                     RGAVDVHDADVVSVRAMMPFA"
     gene            complement(1356..1892)
                     /gene="infC"
                     /locus_tag="VM406_04045"
     CDS             complement(1356..1892)
                     /gene="infC"
                     /locus_tag="VM406_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HVL75164.1"
                     /translation="MKETAIATDKSHRINGEIHAPQVRLNGVDNEPLGIVNLAEAFRL
                     AEEANVDLVEIAPNAQPPVCRLMDYGKFKYQEQKRAHEAKLKQKVIQVKEVKFRPGTD
                     DGDYNIKLRNLTRFLEEGDKTKITLRFRGREMAHQEIGMRMLERLKADLEPFGQVEQF
                     PKMEGRQMVMVLAPKKKK"
     gene            complement(1922..3829)
                     /gene="thrS"
                     /locus_tag="VM406_04050"
     CDS             complement(1922..3829)
                     /gene="thrS"
                     /locus_tag="VM406_04050"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HVL75165.1"
                     /translation="MVSVRLPDGSQRQFEGPVTVAEVASSIGAGLAKAALAGRVDGKL
                     VDTSYTIERDAELAIVTDKDPDGLEVIRHSTAHLLAYAVKELFPDAQVTIGPVIDNGF
                     YYDFSYKRPFTPEDLQAIEKKMAELAKKDEPVTRKVLPRDEAVAYFKSIGEKYKAEII
                     ESIPQGEDVSLYSEGNFTDLCRGPHVPSTGKLKVFKLMKLAGAYWRGDAKNEMLQRIY
                     GTAWAKKEEQEAYLHMLEEAEKRDHRKLGRALDLFHFQDEAPGLVFWHPKGWTVWQQV
                     EQYMRRVYRESGYQEVKAPQILDRSLWEQTGHWQNYRENMFVTESENRNYALKPMNCP
                     GHVQIYSSGLRSYRDLPLRFGEFGQCHRNEPSGALHGLMRVRGFTQDDGHIFCTEEQI
                     QGECTAFHQQAMAVYKDFGFSQIAIKLALRPENRIGSDETWDHAEETLRAALRACGVS
                     WEELPGEGAFYGPKIEYHLKDSLGRPWQVGTMQVDFSMPGRLGAEYVADDNTRKVPVM
                     LHRAIVGSLERFIGILIENHAGALPIWLAPVQVAVLNISDAQSDYAQAVAQNLKKQGF
                     RVHLDLRNEKITYKIREHSIQKLPYIVVIGDKERDANTVAVRARGNVDLGVMSVDALV
                     ERLRNEVDTKA"
     gene            complement(4024..4100)
                     /locus_tag="VM406_04055"
     tRNA            complement(4024..4100)
                     /locus_tag="VM406_04055"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4064..4066),aa:Val,seq:gac)
     gene            4165..5274
                     /locus_tag="VM406_04060"
     CDS             4165..5274
                     /locus_tag="VM406_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HVL75166.1"
                     /translation="MLKHATINRLPARRFRESAWIHRWQKVCFVAAIPPSHKRPEANM
                     SRHNRHSSGSSPSASDLQAHRAGDALHGHAIEGRDPRVLGAAVLLTFMFAAVEAITGF
                     LANSLALIADAGHMVTDSMALLLALFAQMLARRPPSPRHSFGYGRSEPLAAFINGLLM
                     LGVVAWILYEAVQRFMDPPEVRGMMVMIVAAIGLAINAAIAWILSRDRSNINTRAALV
                     HVMGDLLASVAAITSGAVIYLTGWMMVDPVLSVMVSLLILRSTAVVLRDAYHVLMVGV
                     PLHIDYVQVGADITAVPGVLSVHDLHVWEMAAGQPALIGHVEINDLQDWPRIRTEIDA
                     MLLARHGIDHVTIQPESSSMMSGEAPIASAPRSRH"
     gene            complement(5243..7495)
                     /locus_tag="VM406_04065"
     CDS             complement(5243..7495)
                     /locus_tag="VM406_04065"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HVL75167.1"
                     /translation="MVSVAATQTDAHAQLVAGLNADDAARVRAAAHFVEPLYRGKTVA
                     AGQDTFAFLLGVATTLATLNTDADTRLAALLYELPVVDVDAAAQIAERFGEGTAALVD
                     GIRQLMRLHDQAFGYQEVGKGKNATQQAAEQLETLRKMLLAMASDMRVVLVRLASRLA
                     TLRYFAEQKCENETTRSYARETMDLYAPLANRLGIWQLKWELEDLAFRFLEPATYKRI
                     AKMLEEKRLEREGFVEHAIVRLKEALSTAGIKAEVYGRPKHIYSIWSKMRGKSLNFSE
                     LYDVRAFRVIVDDIKDCYAVLSLVHDLWTPIAEEFDDYISRPKPNGYQSLHTVVVAED
                     GRALEVQIRTQDMHRFAEYGVASHWRYKESGGSNFSAQKYDEKVAWLRQLLAWKSEVA
                     DAVVGHEEMQREWVEKLKSATLDDRIYVMTPQARVIELPRGATPIDFAYHLHTDIGHR
                     CRGARVDGTMVPLNTQLKNGQTVEIITARGGVSAGPSRDWLTPGYVASPRTRAKVRAW
                     FNAIDQQETLSRGRGMVEKTLQREGKTATNLEELAQKLGFAKVDELFLAVGKDEFSPR
                     TIEQALHADEAPKASEADLDPALLPNKSRASSVAQGARSGVLVVGTDGLLTQLARCCK
                     PAPPDEIVGFITRGKGVSIHRATCKNLAQMRHKAGERVIQTTWGEPGRDTVYPVDIFI
                     LAADRQGLLRDISEVFSREKINVIGVSTQSAKGQARMAFTAEISSTAQLQKAIGIIRE
                     VSGVTEVQRQ"
     gene            complement(7501..7851)
                     /locus_tag="VM406_04070"
     CDS             complement(7501..7851)
                     /locus_tag="VM406_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HVL75168.1"
                     /translation="MNIERLHVGEKLSEVAIHNGVVYLAGQVAEDLSQDIAGQTREVL
                     GHVDRLLAEAGSDKSRILMCQIFLADLNDIAAMNAVWMEWVAPGNSPPRATVQALLAK
                     PEWRIEMVVTAAQK"
     gene            complement(7873..8739)
                     /locus_tag="VM406_04075"
     CDS             complement(7873..8739)
                     /locus_tag="VM406_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL75169.1"
                     /translation="MNAPRIRFVQCLSPAGLHRVAYKEWGAADNPRVLICVHGVTRVS
                     DDFDRLAAAMSDTYRVICPDIVGRGRSDWLRDAKHYAVPQYVSDMVTLVARADAQTVH
                     WFGTSMGGLIGMGLAGLPGSPIEKLILNDVGPALNPAALARIGQYIGQPVRFASFEDG
                     ERYIREISRPFGGHTDEEWRKFASDVLRREDDGQWRLHYDPDIAAPLRASDAQSMARA
                     EAALWQAYDAIKCPTLLVRGAQSDLLTAETARTMTERGPRAQLVEIPEVGHAPSFLHD
                     DQIAIARRFLLG"
     gene            8861..>8952
                     /locus_tag="VM406_04080"
     CDS             8861..>8952
                     /locus_tag="VM406_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HVL75170.1"
                     /translation="MHKGRTALVTGSTSGIGLGIAREFAAQGAN"
BASE COUNT         1544 a         2899 c         2626 g         1883 t
ORIGIN      
        1 atgtagaacg tgtcctgcat cgaacgggcc gggtgattct ccgggctgtt gagtgctgtg
       61 aaattggtcc agtcggtctc gatctcgggg ccgtccgcga cgtcgaaacc tatcgaaccg
      121 aaaatctgtt cgatacgctc ccaggtgcgc atcaccgggt ggatgccgcc tacgccccgt
      181 ccgcggcccg gaagcgtcac gtcgatcgcc tcggcattca gccgcgcctg catctgcgca
      241 ttggccagct cctcgcgccg ggcagaaagc gcctgctcga tctgttcctt ggctgcgttg
      301 atcaccgcgc cctgggcctt gcgctcctcg ggcgcgagct tgccgaggcc cttcatctgc
      361 tcggtgatct gtccggtctt gccaaggtac ttcgccttgg cgttctcaag tgccgcgccg
      421 tcttcggcgg ccgtgaaatc ggcctgcgcc tgcgcaacga tgtttacgag ggagttcatg
      481 cgactttttc gcccgtgagt gtgatacgaa tccgcaaacg cgcatcggat gggatccgcc
      541 aaaataaaac ggggcatcgg tcaagacctt tgccccgctc aatggcggca ccgcctgacg
      601 acggcgccca cgtcgactac gtcaagcagc gatgctggcc ttcacctgat tgacaatcgc
      661 ggcaaacgcc ggcttgtcgg tcacggccat gtcggccaga accttgcggt caaggccgat
      721 gttggccttc ttcaggccgt tcatgaacac gctgtaggtg acgccgtgcg cccggctcgc
      781 ggcgttgata cgcgcgatcc acaatgcgcg gaacacgcgc ttcttgttgc ggcgatcgcg
      841 ataggcgtat tgcccggcgc gcatgaccgc ctgcttggcg atacggtaga cggtgctgcg
      901 acggccgcgg taacccttgg cctggtcgag aactttctta tgacgggccc gtgcggtgac
      961 cccacgtttt actcgaggca tggtagctcc ttgatagtga ggttaagcga acggcatcat
     1021 tgcgcgcacg gacacgacat cagcgtcatg cacgtccact gcaccgcgca gctggcgctt
     1081 gttcttggtg gttttcttgg tcaggatgtg acgcttgaag gcttggccgc gcttgacggt
     1141 tccgcccggg cgcacgcgaa aacgcttctt cgcgctgctc ttggttttca tcttcggcat
     1201 agcaaatccg ccctcgcggg cggctccggt tttagcatga ttgcaggtgg caatacttca
     1261 ttgcacttgg atgcctgcac tcacttgtta tgcaagggaa tcgagtcccc tgccatctgc
     1321 ccgccccgct cgcgcggcgc gcgctgattc cttgcttact tctttttctt cggggccagc
     1381 accatcacca tctggcgccc ttccatcttc ggaaactgct cgacctggcc aaacggttcc
     1441 agatcggcct tcagtcgctc gagcatgcgc atcccgattt cctggtgcgc catctcgcgg
     1501 ccgcggaatc gcagcgtgat cttcgtcttg tcgccttcct cgaggaaccg ggtcaggttg
     1561 cgcagcttga tgttgtaatc gccatcgtcg gtacccggcc ggaatttcac ttccttgacc
     1621 tggatgacct tctgcttcag cttggcttcg tgggcgcgct tctgttcctg atacttgaac
     1681 ttgccgtagt ccatcaggcg gcacaccggg ggctgcgcat tcggcgcaat ttccaccaga
     1741 tccacatttg cctcttccgc gaggcggaag gcttcagcga ggttgacgat gccaagcggt
     1801 tcgttatcga ccccattcag acgcacttgc ggcgcgtgga tttcaccgtt tatgcgatgc
     1861 gacttgtccg tagctattgc agtttccttt atcaatcaaa taattaagcc gtgcttggcg
     1921 ctcaggcttt ggtgtcgacc tcgttcctga gtcgttccac cagtgcgtcg accgacatca
     1981 ctcccaggtc gacattgccc cgcgcacgca cggccaccgt attcgcatcc cgctccttgt
     2041 cgccgatgac gacgatataa ggcagcttct ggatcgaatg ctcgcgtatt ttataggtta
     2101 ttttctcgtt gcgcaaatcg aggtgcaccc taaacccttg ttttttcagg ttttgtgcca
     2161 cggcttgcgc atagtcagac tgcgcgtccg agatattgag cacggcaacc tgcaccggcg
     2221 caagccagat cggcagcgcg cccgcatggt tttcgatcag aatcccgatg aagcgctcga
     2281 gcgaaccgac gatcgcccgg tgcaacatca ccggcacctt cctcgtgttg tcatcggcca
     2341 cgtactcggc gccgaggcgc cccggcatcg agaaatccac ctgcatcgtt cccacctgcc
     2401 acgggcggcc gagagaatcc ttcaagtggt attcgatctt gggcccgtag aaggcaccct
     2461 cgcccggcaa ctcttcccat gacacgccac aggcgcgcag cgcggcgcgc agggtttcct
     2521 cggcgtggtc ccaggtctcg tcggaaccga tacgattctc ggggcgcagc gccagcttga
     2581 tcgcgatctg ggaaaagccg aagtccttgt agaccgccat cgcctgctgg tggaacgccg
     2641 tgcactcgcc ctggatctgc tcttccgtgc agaagatatg gccgtcgtcc tgcgtgaagc
     2701 cgcgcacgcg catcaatcca tgcagtgcac cggacggctc gttgcgatgg cattgaccga
     2761 actcaccgaa gcgcagcggc aggtcgcggt aactgcgcag gccggaactg taaatctgga
     2821 cgtggccggg gcagttcatc ggcttgagcg catagttgcg gttctccgat tcagtcacga
     2881 acatgttctc gcggtagttc tgccagtgac cagtctgctc ccacaggctg cgatcgagga
     2941 tttgcggcgc cttgacctcc tggtaaccgc tctcccgata cacgcggcgc atgtattgct
     3001 cgacctgctg ccagaccgtc cagcccttcg gatgccagaa caccaggcca ggcgcctcgt
     3061 cctggaagtg gaacaggtcg agcgcgcggc ccagcttgcg gtggtcgcgc ttctcagcct
     3121 cttccagcat gtgcaggtag gcctcctgct cttctttctt cgcccatgcg gtgccgtaga
     3181 tgcgctggag catctcgttc ttcgcatcgc cgcgccagta ggcgccggcc agcttcatca
     3241 gcttgaatac cttgagcttg ccggtcgaag gtacgtgcgg accgcggcac aggtcagtga
     3301 aattgccctc ggagtacagg gagacatctt cgccctgcgg gatcgactcg atgatctcgg
     3361 ccttgtactt ctcaccgatc gacttgaagt aggcgaccgc ctcgtcgcgc ggcaacacct
     3421 tgcgcgtgac cggctcgtcc ttcttcgcca gctccgccat cttcttctcg atcgcctgca
     3481 ggtcttccgg cgtaaacgga cgcttgtagg aaaaatcgta atagaagccg ttgtcgatca
     3541 ccggaccgat ggtgacctgc gcgtccggga acaattcctt caccgcataa gccagcagat
     3601 gggcggtaga gtgacgaatg acttccagtc catcgggatc cttgtccgtc acgatggcca
     3661 gttcggcatc acgttcgatg gtgtaggacg tatcgacaag cttgccgtcg acgcggccgg
     3721 ccagcgcggc cttcgccagg cccgctccga tactcgacgc gacctcggct acggtgacgg
     3781 gcccttcgaa ctggcgctgg gaaccatcgg gaagtcggac tgaaaccata gtgtgctccg
     3841 tggaagatgc ggcaacctgc atcaaaatct gcggacgaaa aaaaacgcgg gctagccgcg
     3901 ttttttccgt tctgatcgaa acgaaaaagc ctgccgacta gcgtcgctcc caaaccattt
     3961 gcgtggtagt tcgcggtgtc ataaccgggc tgcctttctc gctcttacag gtcctgcatt
     4021 cgttggtagg ctcgattgga ctcgaaccaa cgacccctac catgtcaagg tagtgctcta
     4081 accagctgag ctacgagcct aaagaaacga aattatagcg gcaatttttt agattagcta
     4141 gtgcctcatc tcaacgtgtt gcagatgctc aagcatgcaa caatcaaccg actgcccgcc
     4201 cgccgcttcc gggagagcgc gtggatccac cgctggcaga aagtatgctt cgtcgccgca
     4261 atcccgccct ctcacaaacg acccgaagca aacatgtcaa gacacaaccg gcactcatcc
     4321 ggctcaagcc ccagcgcaag cgatctccag gcccaccgtg cgggcgacgc ccttcacggg
     4381 cacgcaatcg aaggccgcga cccgcgtgtg ctgggagcgg ctgtcctcct cacctttatg
     4441 tttgcggcgg tcgaagccat cacgggattc ctggcgaact cgctcgcact gattgccgac
     4501 gcgggacaca tggtgacgga cagcatggca ctcctgctcg ccctgttcgc ccagatgctg
     4561 gcgcgccggc cgccctcgcc aaggcactcg ttcggatacg gccgctcgga accgctggcg
     4621 gccttcatca acggcctgct tatgctcggc gtggtcgcat ggattctgta tgaggcggtg
     4681 cagcgcttca tggacccgcc cgaggtgcgc ggaatgatgg tgatgatcgt tgcggcaatc
     4741 ggccttgcca tcaatgcggc aatcgcctgg atcctctccc gcgatcgcag caacatcaac
     4801 acgcgcgccg cgctggttca tgtaatgggc gacctgctcg cctccgtcgc cgccatcacg
     4861 tccggcgcgg tgatctatct gaccggctgg atgatggtcg acccggtgtt gtcggtaatg
     4921 gtgtcactgc tgatcctgcg ctccaccgcc gtggtcctgc gcgacgccta tcacgtactg
     4981 atggtcggcg tgcccctgca tatcgactac gttcaggtcg gcgccgacat caccgccgtg
     5041 ccgggcgtgc tgtccgtaca cgacctgcat gtctgggaaa tggctgcggg ccagccggcc
     5101 ctgattgggc acgtcgagat caatgacttg caggactggc ctcgcatccg gaccgaaatt
     5161 gatgccatgc tcctcgcccg ccacgggatc gaccatgtca cgattcagcc ggagtcgtcg
     5221 agcatgatgt cgggcgaggc gcctattgcc tctgcacctc ggtcacgcca ctgacttctc
     5281 ggatgattcc gatcgccttc tgcagctgag cggtggatga aatctccgcg gtgaaggcca
     5341 tgcgcgcctg ccctttggcg ctttgggtgc tcacgccgat cacgttgatc ttctcgcgcg
     5401 agaacacttc tgaaatatcc cgcagcaaac cctgccggtc ggcggcaaga atgaagatat
     5461 ccaccgggta taccgtatcc ctgcccggct cgccccaggt ggtctggatc acccgctcgc
     5521 cggccttatg gcgcatctgt gcgagattct tgcaggtggc gcggtggatc gagacgccct
     5581 tgcctcgggt aatgaagccg acgatttcat cggggggcgc cggcttgcag cagcgcgcca
     5641 gctgggtcag caagccgtcg gtgccgacga ccagcacgcc cgagcgcgca ccttgcgcca
     5701 cgctcgatgc tcggctcttg tttggcagca gcgccgggtc gaggtccgcc tcgctcgcct
     5761 tcggcgcctc atcggcgtgc aaggcctgtt cgatcgtgcg cggactgaac tcgtccttgc
     5821 ccacggcgag gaatagttca tccaccttgg caaacccgag tttctgcgcg agctcctcca
     5881 ggttggtcgc ggtcttgcct tcgcgctgca aggtcttttc caccatgccg cggccgcggg
     5941 acagcgtctc ctgctggtcg atcgcattga accaggcgcg caccttggca cgggtgcgcg
     6001 ggctggcgac gtaccccggg gtcagccagt cgcgcgacgg accggcgctg acgccgccgc
     6061 gcgcggtgat gatctcgacg gtctggccgt tcttgagttg cgtattgagc ggcaccatag
     6121 tgccgtcaac ccttgcgccc cggcagcggt ggccgatgtc ggtgtgcagg tgataggcaa
     6181 agtcgatcgg cgtggcgccg cgaggcaact cgatcacgcg cgcttgcggg gtcatcacgt
     6241 agatgcgatc gtccagcgtg gccgacttga gtttctcgac ccattcgcgc tgcatctctt
     6301 cgtgtccgac gaccgcgtcg gcgacctcgc ttttccaggc cagcaactgg cgcagccacg
     6361 cgaccttctc gtcatatttc tgggcgctga agttggagcc gccgctctcc ttgtaacgcc
     6421 agtgcgacgc gactccatat tcggcaaagc gatgcatgtc ctgcgtgcga atctgcactt
     6481 ccaacgcgcg tccgtcctcc gccaccacga cggtgtgcag ggactggtac ccattgggct
     6541 tcggccgcga aatatagtcg tcgaactcct ccgctatcgg cgtccacaga tcgtgcacca
     6601 ggctcagtac ggcgtagcaa tccttgatgt cgtcgacgat cacgcgaaac gcgcgcacgt
     6661 cgtacagctc ggagaagttg agcgacttgc cacgcatctt gctccagatg ctgtagatgt
     6721 gcttgggccg gccgtacact tcggccttga ttccggccgt ggacagtgct tccttcaaac
     6781 gcacaattgc gtgctcgaca aaaccctcgc gctcgaggcg cttttcctca agcatcttcg
     6841 cgatgcgctt gtaggtggcc ggctcgagga aacggaaagc gaggtcttca agctcccact
     6901 tcaactgcca gatgccgagc cggtttgcca gcggcgcata caggtccatc gtctcgcgcg
     6961 cataggagcg tgtggtctcg ttctcgcact tttgttcggc aaaatagcgc agcgtggcca
     7021 gacgggaggc aaggcgaacc agtacaacgc gcatgtcgct ggccatcgcc agcagcatct
     7081 tgcgcagggt ctccagctgc tcggccgcct gctgggtagc gttcttgccc ttgccaacct
     7141 cctggtagcc gaaagcctga tcgtgcagcc gcatcagctg gcgtatgccg tctaccaacg
     7201 cggccgtccc ctcgccgaaa cgttcggcga tctgtgcggc cgcgtcgaca tccaccaccg
     7261 gcagctcgta cagcagtgcg gccaggcgcg tatcggcatc ggtgttgagc gttgccaagg
     7321 tggtcgccac gccaagcagg aaggcaaatg tgtcctgtcc ggccgcgacc gtcttccccc
     7381 ggtataacgg ttcgacgaag tgcgcagccg cgcgcacgcg cgcggcatca tcggcattca
     7441 gcccggcgac cagttgtgcg tgtgcatcgg tttgcgttgc tgcgactgag accattgcgg
     7501 ctatttctgt gcggccgtca ccaccatttc aatccgccat tcgggcttgg ccagcagggc
     7561 ctgaacggtc gcgcggggcg gggaattacc cggcgccacc cactccatcc acaccgcgtt
     7621 catcgccgcg atgtcgttca ggtccgccaa gaagatctgg cacatcagga tgcgcgattt
     7681 gtcgctgccc gcctcggcca gcagccgatc gacgtggccc agcacttccc gtgtctgccc
     7741 ggcgatgtcc tggctgagat cctcggccac ctggccggcg agataaacca cgccgttgtg
     7801 tatcgccacc tcggagagct tttccccgac gtgcagtcgt tcgatgttca tgtttgtcct
     7861 tttccggtct gctcagccga gcaggaaacg tcgcgcgatc gcgatctgat catcatgcag
     7921 gaagctcggt gcgtgcccca cctcgggaat ttccacgagt tgcgcacgcg gtcctcgctc
     7981 cgtcattgtc cgcgccgtct ccgcggtgag caagtccgac tgcgcgccgc gcaccagcag
     8041 ggtcggacac ttgatcgcgt catacgcctg ccacaatgcg gcttccgcgc gcgccatgct
     8101 ctgcgcgtcc gatgcgcgga gcggcgccgc aatatcggga tcgtagtgca gccgccattg
     8161 tccgtcgtcc tcgcgccgca gcacgtcgga ggcgaacttg cgccactcct cgtccgtgtg
     8221 cccgccgaat ggacgggaaa tctcgcgaat ataccgttct ccgtcctcga agctcgcgaa
     8281 tcggacaggc tggccgatgt actggccaat ccgcgcaagt gcggccggat tcagggccgg
     8341 tccgacgtcg ttgaggatca gcttttcaat cggacttccc gggagccccg ccagtcccat
     8401 cccgatgagg ccgcccatcg aggtgccgaa ccagtgcacc gtctgcgcgt ccgcgcgggc
     8461 gacaagagtc accatatcgc tcacgtattg cggcaccgca tagtgtttcg cgtcgcgcaa
     8521 ccagtccgac ctgccgcgcc cgacgatatc cggacagatg acgcggtaag tgtccgacat
     8581 cgccgccgcc agcctgtcga aatcatctga tacccgggtg acgccgtgaa cgcagatcag
     8641 gacgcgcgga ttgtccgcag cgccccattc cttgtaggcg actctgtgca acccggcagg
     8701 ggacaggcat tggacgaatc ggatgcgagg cgcgttcatg ttgttggccg atctggactt
     8761 ttcgcttatc aaacgttcat tttaaggggc gataagctta aaatcctacc tgacttcgac
     8821 gccttacaat ttattctcag gggaaatcat gcagggcact atgcataaag gaaggacggc
     8881 gctggtcacc ggctcgacct ccggcatcgg cttgggcatt gcgcgcgagt tcgccgcgca
     8941 aggcgccaat at
//
LOCUS       DATLGF010000135         6449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_132349, whole genome shotgun sequence.
ACCESSION   DATLGF010000135 DATLGF010000000
VERSION     DATLGF010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6449
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132349"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /gene="tilS"
                     /locus_tag="VM406_04085"
     CDS             complement(<1..1202)
                     /gene="tilS"
                     /locus_tag="VM406_04085"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HVL75171.1"
                     /translation="MSESVEEMFERALEAILARVLASAGPQDAQALAVAYSGGLDSTV
                     LLHLVHAFAARRGLRLFAFHVDHGLSPNARDWAEHCALQCARLGIGFDSRSLALAVGR
                     NIEQEARHARYAALGALCRQHSVVLLLTAHHQDDQAETVLLQLLRGAGVAGLSGMEAA
                     GPAPELLGDGATWIGRPLLGLTRLQLQQWAEQCELAWIEDESNRDARYTRNHLRQQIT
                     PRIGQAFPGYQARIARAAHHAAEAQAVLTDMAALDMQQCADGEGLDLDALRGLSEARL
                     NNLLRYWFARHGLRMPTTAWLAQLRVQAFAAADDARVRITHPDCEVWRYRKRLMLVER
                     IDERVLDALPQTLHWDGQGSIHVPAFGGWLEFTMQGEGVDAGWLRGQTLTIRHRSGGE
                     RLKPARNRP"
     gene            complement(1206..2180)
                     /locus_tag="VM406_04090"
     CDS             complement(1206..2180)
                     /locus_tag="VM406_04090"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HVL75172.1"
                     /translation="MSKTTFLGFEQPIAELEAKIEELRFVQDDSAVDISEEIERLSKK
                     SQQLTKDIYAKLTPWQVSQIARHPQRPYTMDYVNQIFTDFHELHGDRTYADDLSIIGG
                     LARFNGQPCMVIGHQKGRDTKERAMRNFGMPKPEGYRKAMRLMKLAEKFGLPVFTFVD
                     TPGAFPGIDAEERGQSEAIGHNLYVMAELKVPLIATIIGEGGSGGALAIAVGDVVLML
                     QYATYSVISPEGCASILWKTSERAADAADALGLTAHRLKALNLIDKIVNEPLGGAHRD
                     HKQMAALLKRALADSLRQVQGMKPKELIAARHEKLMTYGKFKEIASPE"
     gene            complement(2216..2854)
                     /locus_tag="VM406_04095"
     CDS             complement(2216..2854)
                     /locus_tag="VM406_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HVL75173.1"
                     /translation="MALLIQPATPAYWQSAKEELMRRDRIMRRLIPQFGDMQLAARGE
                     AFVTLARSIVSQQISVKAAESIWQRLLLTCPKCTPAQIVKAGPEALSACGLSKRKVEY
                     VLDLAEHFRARRVHADKWAEMDDEDVIAELIQIRGIGRWTAEMFLIFNLLRPNVLPLD
                     DLGLLKGISVNYFSGEPVSRSDAREVAANWDPWRTVATWYLWRSLDSAPVVY"
     gene            complement(2860..4245)
                     /gene="cysS"
                     /locus_tag="VM406_04100"
     CDS             complement(2860..4245)
                     /gene="cysS"
                     /locus_tag="VM406_04100"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HVL75174.1"
                     /translation="MTALQIYNSLAREKQTFVPLQPGKVRMYVCGMTVYDYCHLGHAR
                     VMVVFDMVQRWLRRSGYDVTYVRNITDIDDKIIKRAVENGEPISQLTRRFIAAMDEDA
                     AALGVQKPDHEPRATEYVPQMLGLIGKLEKNGLAYRAPDGDVNFSVRDFPGYGKLSGK
                     SLDDLQAGTRVELNTGKRDPLDFVLWKSAKASEPDEVKWDSPWGKGRPGWHIECSAMS
                     CELLGAHFDIHGGGEDLQFPHHENEIAQSEGAHRDTFVNYWMHNGFIRVDNEKMSKSL
                     GNFFTIRDVLTKYDAEVVRFFILRAHYRSPLNYSDAHLDDAKNALGRLYTALKEVQGD
                     GGRLDRDEIHARRFADAMNDDFNTPVAFAVLFDLASEVNKTRSPELARQLRELGDVIG
                     LLQREPQEYLKGGASAGLDEARILAQIEARAAAKKAKNFAEADRIRAELLAEGVVLED
                     KPGGLTEWRRA"
     gene            4504..5082
                     /locus_tag="VM406_04105"
     CDS             4504..5082
                     /locus_tag="VM406_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HVL75175.1"
                     /translation="MTLARRTLLKLLASLPLAGAVSIASASEAPQVLLKTSMGDIVLA
                     LDQDKAPKTVANFLQYVKNGHYNGTIFHRVIDGFMIQGGGFLPDMSQKATAAPIENEA
                     KNGLKNVRYSIAMARTAAPHSATAQFFINVRDNTALDHPGHDGWGYAVFGRVIKGQDV
                     VDRIKAVPTTNAGMHQNVPAKPVIIESARIVK"
     gene            5113..5601
                     /locus_tag="VM406_04110"
     CDS             5113..5601
                     /locus_tag="VM406_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HVL75176.1"
                     /translation="MAVILNTNHGAIKIELDADKAPKTVENFIEYVKSGHYDGTIFHR
                     VIDGFMIQGGGFEPGMKQKPTREPIENEAKNGLKNDTYTIAMARTSAPHSATAQFFIN
                     VKNNSFLDYPGQDGWGYCVFGKVVEGQDVVDKIRSVKTTRAGMHADVPVEDVVIEKAE
                     VV"
     gene            5614..6405
                     /locus_tag="VM406_04115"
     CDS             5614..6405
                     /locus_tag="VM406_04115"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HVL75177.1"
                     /translation="MAHSAKAQPQAVALFVSDLHLQDALPRTTDCFLRFLREHAGSAQ
                     RLYLLGDLFDAWPGDDDLAAPLPRRMAGALAEVAASGVATAWIGGNRDFLVGQVFAQE
                     ARLQLLQEPHVVELAGKRLVLLHGDAQCTDDRDYQAFRVQVRDPAWQQAFLARPLAER
                     KAIVANLRERSRAAQRDKTCEIMDVNEAAIAAVFEQTQAEVMVHGHTHRPAVHRVEVA
                     GATRTRIVLPDWDCEQEPARGGWLALLEDGSLRRYRTDGSLEDAV"
BASE COUNT         1173 a         2118 c         2003 g         1155 t
ORIGIN      
        1 ggccggttcc gcgcgggctt gagccgctcc ccgccgctgc ggtgcctaat cgtcaaggtc
       61 tggccgcgca gccatccggc gtccaccccc tccccttgca tggtgaactc cagccatccg
      121 ccgaatgcgg gcacgtggat cgaaccctgc ccgtcccagt gcagcgtctg cggaagcgca
      181 tcgagcaccc gctcgtcgat gcgctccacc agcatcaggc gcttgcgata gcgccagacc
      241 tcgcagtccg gatgcgtgat gcgcacgcgc gcgtcatcgg cggccgcgaa ggcctgcacc
      301 cgcaactgcg caagccatgc ggtcgtcggc atgcgcagcc cgtggcgtgc gaaccagtag
      361 cgcagcagat tgttcaggcg cgcttcgctg aggccgcgca gtgcatcgag atccagcccc
      421 tctccgtctg cgcactgttg catgtcgagc gccgccatgt ccgtgagcac ggcctgggcc
      481 tcggccgcat ggtgcgcggc ccgcgcaatg cgcgcctgat acccggggaa agcctgcccg
      541 atgcgtggcg tgatctgctg gcgcaggtgg ttgcgcgtgt agcgcgcatc gcgattggat
      601 tcatcctcga tccacgccag ctcgcattgc tccgcccatt gctgcagctg aagacgggta
      661 agcccaagca agggacgtcc gatccaggtt gcaccatcgc cgagcagttc cggcgccggc
      721 ccggccgcct ccatgcccga caagccggcc acgccggcgc cgcgcagcag ttgcagcagc
      781 accgtctcgg cctggtcatc ctggtgatgc gcggtcagca gcagcacgac gctatgctgc
      841 cggcacagcg cgccgagggc cgcatagcgg gcgtggcgcg cctcctgctc gatgttgcgc
      901 ccgactgcca gtgcgaggct gcggctgtcg aagccgatcc caaggcgggc gcactggagg
      961 gcgcaatgct ccgcccagtc gcgcgcattc gggctcaatc cgtgatcaac atggaatgca
     1021 aacaggcgaa gcccgcgccg agctgcaaac gcgtgcacca gatgcaggag aacggtggaa
     1081 tcgagcccgc cgctgtaggc gaccgccaat gcctgcgcat cctgagggcc ggcagatgca
     1141 agaacgcgcg ccagaattgc ttccagcgcg cgttcaaaca tttcctccac ggactcgctc
     1201 acggcttact cgggtgaggc gatttccttg aatttcccgt aggtcatcag cttctcgtgg
     1261 cgtgccgcga tcaactcctt cggcttcatg ccctgcacct ggcgcagcga atcggccagc
     1321 gctcgcttga gcagcgccgc catctgcttg tgatcacgat gcgcgccgcc cagcggctcg
     1381 ttgacaatct tgtcgatcag gttcagggcc ttcaggcggt gcgcggtcag gccgagcgca
     1441 tcggcggcgt cggccgcacg ctctgaggtc ttccacagga tcgacgcgca gccttccggc
     1501 gagatcaccg aataggtcgc gtattgcagc atcagcacca cgtcgccgac cgcgatcgcg
     1561 agtgccccgc ccgagccgcc ctcgccgatg atggtggcga tcaggggcac cttcagttcg
     1621 gccatcacat acaggttgtg accgatcgct tcggactggc cgcgttcttc cgcatcgatg
     1681 ccgggaaatg cgccgggcgt gtcgacgaag gtaaacaccg gcaggccgaa cttctcggca
     1741 agcttcatca ggcgcatggc cttgcgatag ccttccggtt tgggcatgcc gaagttgcgc
     1801 atcgcgcgct ccttcgtgtc gcgccccttc tggtggccga tcaccatgca cggctggcca
     1861 ttgaagcgtg ccaggccgcc gatgatggac aggtcgtcgg cgtaggtgcg atcgccgtgc
     1921 aactcgtgga agtccgtgaa gatctggttc acgtagtcca tcgtgtacgg gcgctgcgga
     1981 tgacgcgcga tctgcgacac ctgccagggg gtgagcttgg cgtagatgtc cttggtcagt
     2041 tgctggctct tcttcgagag ccgttcgatt tcctcggaga tgtcgaccgc ggaatcatcc
     2101 tgtacgaagc gcagttcttc gatcttcgct tccagttccg cgatcggctg ttcaaagccg
     2161 agaaaggtag tcttgctcat cgagcctcca cataccggcg catgccggca cttgcttaat
     2221 aaacgacggg cgcagaatcc agactgcgcc acaggtacca ggtggcgacc gtgcgccacg
     2281 ggtcccagtt ggcggccact tcgcgcgcat cgctgcgcga gaccggctcg ccggaaaaat
     2341 agttgacgct gattcccttc aacaacccga ggtcatcgag cggaaggacg ttcggccgga
     2401 gcagattaaa tatcagaaac atctcggctg tccagcggcc gatgccgcgg atctggatca
     2461 gttccgcaat gacatcttcg tcgtccatct ccgcccactt gtccgcatgc acgcggcgcg
     2521 cacggaagtg ctcggccagg tcgagcacgt actcgacctt gcgcttggac agcccgcacg
     2581 ccgaaagtgc ctccggtccc gccttcacta tctgggcggg cgtgcatttc ggacaagtca
     2641 gaagcaggcg ttgccaaatc gactcggccg ccttcaccga tatctgctgg gaaacgatgg
     2701 aacgggcgag cgtgacgaag gcctcgccgc gcgcagccag ttgcatatcg ccgaattggg
     2761 gaatgagccg gcgcatgatg cggtcgcgcc gcatcagctc ctccttcgcg ctctgccagt
     2821 aggcaggcgt agcgggttgg atcagcaagg ccatgtggct tacgccctgc gccactcggt
     2881 caggccgccc ggtttgtcct cgagcaccac gccctcggcc agcaactcgg cgcggatgcg
     2941 atccgcctcc gcgaagtttt ttgccttctt ggccgctgca cgggcctcga tctgtgcgag
     3001 gatgcgtgcc tcgtccaggc cggccgaagc accgcctttc aggtattcct gcggctcgcg
     3061 ctgcagcaat ccgatcacgt cgcccaactc cctgagctgg cgcgccagct caggtgagcg
     3121 cgtcttgttc acttcgcttg ccaggtcgaa cagcaccgca aatgcgaccg gggtgttgaa
     3181 gtcgtcgttc atcgcgtcgg caaaacggcg cgcatggatt tcgtcacggt cgagccgacc
     3241 gccgtcgccc tgcacttcct tgagcgcggt gtacaagcgg ccgagcgcgt tcttcgcgtc
     3301 gtccagatgc gcgtccgaat agttcagcgg gctgcgatag tgggcacgca ggatgaagaa
     3361 acgcacgact tcggcgtcgt acttcgtcaa cacatcacgg atcgtgaaga aattgccgag
     3421 cgacttcgac atcttctcgt tgtcgacccg gatgaaaccg ttgtgcatcc agtagttgac
     3481 gaaggtgtcg cgatgcgcgc cctcggactg ggcgatttcg ttctcgtgat gggggaactg
     3541 cagatcttcg ccaccgccgt ggatgtcgaa atgcgcaccg agcagttcgc agctcatggc
     3601 cgagcactcg atatgccagc cgggtcgtcc cttgccccac ggcgaatccc acttcacctc
     3661 gtccggctcg gatgccttgg cggacttcca cagcacgaag tcgagcggat cgcgcttgcc
     3721 ggtattgagt tcgacgcgcg tgccggcctg gaggtcgtcg agcgacttgc cggacagctt
     3781 gccgtagcca gggaagtcgc gcacggaaaa attcacgtca ccgtcggggg cgcgataggc
     3841 caggccgttc ttctcgagct tgccgatcag gcccagcatt tgcggcacgt attcagttgc
     3901 gcgcggctca tgatcgggct tctgcacgcc cagcgcggcc gcgtcctcgt ccatcgccgc
     3961 aatgaagcgc cgggtcagct gcgaaatcgg ctcgccattc tcgaccgcgc gcttgatgat
     4021 cttgtcgtcg atatcggtga tgttgcgcac ataggtgacg tcgtatcccg aacggcgcag
     4081 ccagcgctgc accatgtcga acaccaccat cacgcgcgca tgtccgaggt ggcaataatc
     4141 gtagaccgtc attccacaga catacatgcg caccttgccc ggttgtaaag gaacaaaggt
     4201 ctgcttctcg cgcgcgagcg agttgtaaat ctggagtgca gtcatggagt cgtatcgggc
     4261 ttggcggact tgcttggagt tggaatcagg cgcagcgggc acatcggaca gcaccatccg
     4321 gataccgcgg cggcgacagc cttgacaggg aggcagtgcc gggcgcgcgg accactgcta
     4381 gaattgaccg cttcgccgca gtatagcatc gccgaaagac gccctcccgc ccctcccgcc
     4441 gtggctgccg gggcacggaa gatacacaaa cacgattttt ctgtaaagca cgaggacata
     4501 cgcatgacgc ttgcgcgccg cacccttctg aagctgctcg cctcacttcc gctcgccggt
     4561 gccgtcagca tcgcatccgc cagcgaagcg ccccaggtgc tcctgaagac cagcatgggc
     4621 gacatcgtgc tcgcgctgga ccaggacaag gcgccgaaga ccgtcgcaaa ctttctccaa
     4681 tacgtgaaga acggccatta caacggcacg atctttcatc gcgtgatcga cggcttcatg
     4741 atccagggcg gcggattcct ccctgacatg agccagaagg cgaccgcggc gccgatcgag
     4801 aacgaggcga agaacggcct gaagaacgtt cgctattcga tcgcaatggc gcgcaccgcc
     4861 gcgccgcatt ccgcgaccgc ccagttcttc atcaacgtgc gcgacaacac ggcgctcgac
     4921 catcccggcc acgacggctg gggctacgcg gtgttcggcc gggtgatcaa gggccaggac
     4981 gtggtcgacc gtatcaaggc cgtgcccacg acgaacgccg gcatgcacca gaacgtaccg
     5041 gccaagccgg tcatcatcga gtcggccaga atcgtcaagt aagctatttt caacccgtac
     5101 aggagccccg acatggcagt catcctcaat accaaccacg gtgccatcaa gatcgagctc
     5161 gacgccgaca aggcgccgaa gaccgtcgaa aactttatcg aatacgtgaa gtccggccat
     5221 tacgacggca cgatcttcca tcgggtgatc gacggcttca tgatccaggg cggcggcttc
     5281 gaacccggca tgaagcagaa gcccacacgc gagccgatcg agaacgaagc caagaacggt
     5341 ctgaagaacg atacctacac gatcgcaatg gcgcgcactt cggccccgca ttcggccacc
     5401 gcgcagttct tcatcaacgt gaagaacaac agcttcctcg actacccggg ccaggacggc
     5461 tggggctact gcgtgttcgg caaggtggtg gaaggtcagg atgtggtcga caagatcagg
     5521 tcggtcaaga ccacgcgtgc cggcatgcac gccgatgtgc ctgtcgagga cgtggtgatc
     5581 gagaaggccg aggtcgtctg agtgccgacg ggcatggctc actccgcgaa agcgcaacct
     5641 caggcggttg cgcttttcgt ttcggatctg catctgcagg atgcgctgcc gcgcacgacg
     5701 gactgtttcc tgcgcttcct gcgcgagcac gcagggtcgg cgcagcgtct atatctgctc
     5761 ggcgacctgt tcgacgcctg gccgggcgat gacgatctcg ccgcgccgct tccgcggcgc
     5821 atggccggcg cattggccga agttgcagcc tctggcgtcg ccaccgcctg gatcggtggc
     5881 aaccgcgatt tcctggtcgg ccaagtcttc gcgcaggaag cccgcctgca attgctgcag
     5941 gaaccgcacg tggtggaact ggcaggcaag cgccttgtac tgctgcatgg cgatgcgcaa
     6001 tgcaccgacg accgcgacta ccaggccttc cgcgtgcagg tgcgcgatcc tgcctggcag
     6061 caggccttcc tcgcgcgccc gctggcggag cgcaaggcaa tcgtcgcaaa cctgcgcgaa
     6121 cgcagccgcg cggcgcagcg cgacaagacc tgcgagatca tggatgtgaa cgaagcggcg
     6181 atcgccgccg tgttcgaaca aacgcaggcc gaggtgatgg tgcatggtca cacccatcgc
     6241 cccgcggtgc atcgcgtgga agttgccggc gccacacgca cgcgcatcgt gcttccggac
     6301 tgggattgcg aacaggagcc cgcccggggc ggctggctgg cgctgctgga ggacggcagc
     6361 ttgcgccgct atcgcaccga cggctcgctc gaagacgccg tctgaatttg caacgaaccg
     6421 ccgccttatt cgaactgctt cctgaagcc
//
LOCUS       DATLGF010000136         4390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_52573,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000136 DATLGF010000000
VERSION     DATLGF010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52573"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /gene="aspS"
                     /locus_tag="VM406_04120"
     CDS             <1..1064
                     /gene="aspS"
                     /locus_tag="VM406_04120"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HVL75178.1"
                     /translation="RDLFEGMIRGVFKNVLDVDLPNPFPVMPYSEAMGLYGSDKPDMR
                     VKLQFTELTEQMKDVEFKVFSGAANMAGGRVVALRVPGGGAMPRSEIDAYTQFVAIYG
                     AKGLAWIKVNEKAKGRDGLQSPIVKNIHDAGLAAILEKTGAQDGDLIFFGADKAKVVN
                     DAIGALRVKIGHSEFGRKNGLFEDTWRPLWVIDFPMFEYDEDEQRYTAAHHPFTSPKD
                     GHEDLLETDPGRALAKAYDMVLNGWELGGGSVRIHRADVQSKVFRALKIEAEEAQLKF
                     GFLLDALQYGAPPHGGLAFGLDRLVTMMAGAESIRDVIAFPKTQRAQCLLTQAPSEVD
                     EKQLRELHIRLRNIEAAKA"
     gene            1110..1595
                     /gene="nudB"
                     /locus_tag="VM406_04125"
     CDS             1110..1595
                     /gene="nudB"
                     /locus_tag="VM406_04125"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HVL75179.1"
                     /translation="MNRPYKIPESVLVVIHTAELEVLLIERADKPGFWQSVTGSKDAP
                     EEPLEATAIREVFEETGIRVVDRPLAANEVAPDCLTDWQLSNVYEIYPVWRHRYAPGV
                     TRNTEHVFGLRVPRNTPIALAPREHLNHLWLPYREAADRCFSPSNAEAILQLPRHIAG
                     G"
     gene            1681..2499
                     /locus_tag="VM406_04130"
     CDS             1681..2499
                     /locus_tag="VM406_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HVL75180.1"
                     /translation="MKIRIATYNIHKGVSALRSVPRVHALKQALASMEADVLFLQEVQ
                     GRHDKYATRHAALWPEQAQHHYLAGESHHAVYGMNAVYDHGHHGNALLSRYPVLSSRN
                     QDVSDHAYESRGILHCVVQAPACDLHCYVIHLGLFAGSRRRQTEALVSAVLSSSPPNA
                     PLIIAGDFNDWTNQLSATLRKELGVTEVFDTNLPTRGFGAYLRTLSGRGPNVLPARTF
                     PAALPWLRLDRIYVRGFSVDSAQVLHGALWARLSDHAPIVASLELKMTQSAYGT"
     gene            2582..3748
                     /gene="clsB"
                     /locus_tag="VM406_04135"
     CDS             2582..3748
                     /gene="clsB"
                     /locus_tag="VM406_04135"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HVL75181.1"
                     /translation="MPPVEFTAGNEVRLLHCGAEYFPALIAAIDAARREIFLETYIFA
                     LDHTGNEVKDALARAAARGVSVSVTTDWLGSGRAACALLDDYFRQHRIRYRVFNPWFT
                     RGVARMHRKVCVVDAQVAFLGGLNINFDYVSDDGFAVPLPAPRWDFAVMVRGPLVPII
                     HREVEAQWLYQDGLHLRFRFERFLENQRRRVPPFDEPVLAAFVYRDNLRNRRTIQRSY
                     LKALGAARRSALLANPYFAPNRKLRDGLAAAARRGVEVTLLIGVGQFRIQDAVAHSFY
                     PKLLKSGVKVVEYRKTQLHGKVAVIDDDWATVGSSNWDGLSLFVNHEANVIVRDQAFA
                     RDLRRQIDRAVAEGVVVRMEDFANIPWYERAWYGMAALIYKTVLRVITRGRYTE"
     gene            complement(3736..>4390)
                     /locus_tag="VM406_04140"
     CDS             complement(3736..>4390)
                     /locus_tag="VM406_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVL75182.1"
                     /translation="RQRADERVVTVTSVPSLVARWLLPRLGRLQRCHPDIDMRVLASL
                     HPVDLVHEGIDVAIRLGTGPYPGLQHDVLMEERFSAVCSPAFLTAAGDLRTPADLLRQ
                     PLLHDELEPRIPDEVNWVRWLRHFGVVYKGPTRPSFSHTYLTLEAAANGQGIAVAADV
                     FVEEDLRNGRLVRVFEERVTGPYRFHLLRALHAEARPAVKAFCDWLLEEARESSYSV"
BASE COUNT          750 a         1459 c         1433 g          748 t
ORIGIN      
        1 tccgcgacct gttcgaaggc atgatccgcg gcgtgttcaa gaacgtgctg gacgtcgacc
       61 tgccgaaccc gttcccggtg atgccgtact cggaagcgat gggcctgtac ggctcggaca
      121 agcccgacat gcgggtgaag ctgcagttca ccgagctgac cgagcagatg aaggacgtcg
      181 agttcaaggt gttctccggt gcggccaaca tggctggcgg gcgcgtcgtc gcgctgcgcg
      241 tgccgggtgg cggcgcgatg ccgcgctcgg aaatcgacgc ctatacgcag ttcgttgcga
      301 tctacggcgc caagggcctg gcctggatca aggtcaacga gaaggccaag ggccgcgacg
      361 gcctgcagtc gcccatcgtc aagaacatcc atgatgccgg cctggccgcc atcctggaga
      421 aaaccggcgc gcaggacggc gacctgatct tcttcggcgc cgacaaggcc aaggtcgtca
      481 acgacgcgat cggcgcgctg cgcgtgaaga tcggccacag cgaattcggc cgcaagaacg
      541 gcctgttcga ggatacgtgg cgccctctgt gggtgatcga tttcccgatg ttcgagtacg
      601 acgaggacga gcagcgctac accgccgccc accacccgtt cacctcgccg aaggacggcc
      661 acgaagacct gctcgaaacc gatccgggcc gtgcgctggc caaggcctac gacatggtgc
      721 tgaacggctg ggaactgggc gggggctcgg tgcgtatcca ccgcgccgat gtgcagagca
      781 aggtgttccg cgcgctgaag atcgaggccg aagaagcgca gctcaagttc ggcttcctgc
      841 tcgatgcgct gcagtacggc gcgccgccgc atggcggcct ggccttcggc ctggaccgcc
      901 tggtcacgat gatggccggc gccgaatcga tccgcgacgt gatcgctttc ccgaagaccc
      961 agcgcgcgca gtgcctgctg acccaggcgc cgtcggaagt cgacgagaag cagttgcgcg
     1021 agttgcatat ccgcctgcgc aacatcgagg ccgccaaggc ctgagccgcc ggcgcgtccc
     1081 tccgtgcgcg gcacggaggg aaccttcgga tgaaccgacc ttacaagatc cccgaatcgg
     1141 tgctcgtcgt gatccacacg gcagagctcg aggtcttgct gatcgagcgc gccgacaagc
     1201 ccggcttctg gcaatcggtg accggttcga aggatgcgcc ggaggagccg ctggaagcga
     1261 ccgcgatccg cgaggttttc gaggaaaccg gcattcgcgt cgtcgatcgc ccgcttgcgg
     1321 caaacgaggt cgcgcccgat tgcctgaccg actggcagct ctccaacgtc tacgagatct
     1381 atccggtctg gcgccatcgc tatgcaccgg gcgtgacccg caacaccgag catgtattcg
     1441 gcctgcgcgt gccgcgcaat acaccgatcg cgcttgcgcc gcgcgagcat ctgaaccacc
     1501 tatggctccc ctatcgcgaa gcggcggacc gctgcttctc tccttccaac gccgaagcga
     1561 tcctgcaact gccccgccac atcgcgggcg gctgaaactt gttctgcgtc aagccggtca
     1621 gattcatatc ttggttccgg cgggcccgtc ccgcattttc ccggcagcat acataaggac
     1681 atgaagatca ggatcgcgac ctacaacatc cacaagggcg tcagcgcgct gcgcagcgtt
     1741 ccccgcgtcc atgctttgaa gcaggccctg gcttcgatgg aggccgacgt gctgttcctg
     1801 caggaggtgc agggcaggca tgacaagtac gcgacgcgcc atgcggcgct gtggcccgag
     1861 caggcccagc atcattacct ggccggcgag tcgcatcatg cggtgtacgg aatgaacgcg
     1921 gtgtacgacc acggccatca cggcaacgcc ttgctcagcc gttatccggt cctgtcctcg
     1981 cgcaaccagg atgtttcgga ccacgcctac gagtcgcgcg gcatcctgca ctgcgtggtg
     2041 caggcgccgg cctgcgacct gcactgctat gtgatccatc tcggcctgtt cgccggcagc
     2101 cggcgccgcc agacggaggc cctggtgtcg gcggtgttgt catcctcgcc tcccaatgcg
     2161 ccgctgatca tcgccggcga tttcaacgac tggaccaacc agttgagcgc gaccttgcgc
     2221 aaggaactcg gcgtgaccga ggtgttcgac accaatctgc ccacgcgcgg cttcggtgcc
     2281 tatctgcgca cgctctccgg gcgcgggccg aacgtgttgc cggcccggac ctttccggct
     2341 gcgctgccct ggctgcggct ggaccgcatc tatgtgcgcg gcttcagcgt cgactcggcc
     2401 caggtcctgc acggcgcgct gtgggcccgg ctgtccgatc atgcgccgat cgtcgcctcg
     2461 ctcgaactga agatgacgca gtcggcctac ggcacctgag cgggacttgt cacccgccaa
     2521 gagttgacca tgggtgacgg ggtagaatcg cagccatttc atcgggagtt cagccgcatt
     2581 catgccaccg gtcgagttca ccgccggcaa cgaggtgagg ctgctgcatt gcggcgctga
     2641 gtacttccca gcgttgatcg cagcaatcga cgccgcccgc cgcgagatct ttctcgagac
     2701 ttacatcttc gccctcgacc ataccggcaa cgaggtcaag gatgcgctcg cgcgcgcggc
     2761 ggcgcgcggc gtgtcggtca gcgtcacgac agactggctg ggaagcggcc gcgccgcctg
     2821 cgcgctgctc gacgattact tccgccagca ccggatccgg tatcgcgtct tcaacccctg
     2881 gttcacgcgg ggtgtggcgc gcatgcaccg caaggtgtgc gtggtcgatg cgcaggtcgc
     2941 cttcctcggc ggactcaaca tcaatttcga ttacgtctcg gacgacggct tcgctgtgcc
     3001 gctaccggcg ccgcgctggg atttcgcggt gatggtgcgc ggcccgctgg tgccgatcat
     3061 ccaccgcgaa gtcgaggcgc agtggctgta ccaggacggc ctgcacctgc gttttcgctt
     3121 cgaacgcttc ctcgagaatc agcggcgccg cgtgccgccg ttcgacgagc cggtgctggc
     3181 ggccttcgtt tatcgcgaca atctgcgcaa ccggcgtacg atacagcgtt cctatctgaa
     3241 ggcgctcggt gcggcgcggc gcagcgcctt gctcgccaac ccctacttcg cccccaatcg
     3301 caagttgcgc gacgggctgg ccgccgccgc gcggcgcggc gtggaggtga cgctcttgat
     3361 cggcgtcggc cagttccgca tccaggatgc ggtcgcgcat tcgttctatc cgaagctgct
     3421 caagagcggc gtgaaggtgg tcgaataccg caagacccag ttgcacggca aggttgcggt
     3481 aatcgacgac gactgggcga ccgtcggttc aagcaactgg gacggactgt cgctgttcgt
     3541 caaccacgaa gcgaacgtga tcgtgcgcga ccaggcgttc gcgcgcgacc tgcgccggca
     3601 gatcgaccga gcggttgcgg aaggcgtggt ggtacggatg gaagacttcg ccaacattcc
     3661 ctggtacgag cgcgcctggt acggcatggc cgcgctgatc tacaagaccg tgctgcgcgt
     3721 gatcacgcgc gggcgctata ccgagtagct cgattcgcgt gcctcttcca gcagccagtc
     3781 gcagaacgcc ttcaccgccg gccgcgcctc ggcatgcaag gcgcgcagca ggtgaaaacg
     3841 gtagggaccg gtcacccgtt cttcgaagac gcgcaccagg cggccgttgc gcagatcctc
     3901 ctcgacgaag acgtccgccg ccaccgcgat gccctgcccg ttggccgccg cctccagcgt
     3961 gagataggta tgcgagaagc tgggccgggt cggacccttg tagacgacgc cgaaatggcg
     4021 cagccagcgg acccagttca cttcgtcggg gatgcgcggt tcgagttcgt catgcagcag
     4081 cggctggcgc agcaggtcgg ccggcgtgcg caggtcgccg gccgccgtga gaaaggccgg
     4141 actgcatacg gcggaaaaac gttcctccat cagcacgtcg tgctgcaggc cgggatacgg
     4201 tccagtgccg aggcggattg ccacgtcgat gccctcgtgc acgagatcca ccggatgcag
     4261 cgaggccagc acccgcatgt cgatgtccgg atggcagcgc tgcagccggc ccaggcgcgg
     4321 cagcagccag cgcgcaacaa gcgatggcac tgacgtcacg gtcaccacgc gttcgtcggc
     4381 gcgctgtctg
//
LOCUS       DATLGF010000137         3921 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_66717,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000137 DATLGF010000000
VERSION     DATLGF010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66717"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1343)
                     /gene="pgi"
                     /locus_tag="VM406_04145"
     CDS             complement(<1..1343)
                     /gene="pgi"
                     /locus_tag="VM406_04145"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HVL75183.1"
                     /translation="MSHIALTHYPAWTALAEHFETAKDWGLRALFERQPGRFEAFSLE
                     AAGLLLDYSKNRVDADTMRLLLQLARERHVEQRRDAMFAGAKINVTERRSVLHAALRM
                     PRSRSLVVDGQDVIPEVHAVLDRIQAFTERVRSGEWVGGTGARITDIVNIGIGGSDHG
                     PEMVCRALRPFVQLGLTLHFVANVDGHDLDAVLQRVNPETTLFVVASKTFTTRETMMN
                     AESARAWFTARRPVNELPRHFVAVSTNTSAVRDFGIDPENMFPFWDWVGGRYSVWSAI
                     GLPVALAIGFERFAEFLAGAHAMDEHFRTAPLEHNMPVVLGMIGIWYRNFFRWPSISI
                     APYLQDLSQFPAYLQQLDMESNGKRVTLAGDEAEVASGPIVWGDVGTNGQHAYFQLLH
                     QGTDTTPVDFIAALKPAHALPGHHAVLLANCLAQAEAFMRGKNADEARADMQAQG"
     gene            1593..3533
                     /gene="edd"
                     /locus_tag="VM406_04150"
     CDS             1593..3533
                     /gene="edd"
                     /locus_tag="VM406_04150"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HVL75184.1"
                     /translation="MPIHSTLAAVTQRITERSRPFRQSYLARMDAARVQGVHRGTLAC
                     TNLAHGFAAFPANDKLRLKQQRVPSVAIVSAYNDMLSAHQPFERFPKIIKDAVREAGG
                     VAQFAGGVPAMCDGVTQGQPGMELSLFSRDTIAMGTAVALSHNMFDAALYLGVCDKIV
                     PGLVIGALHFGHLPAVFIPAGPMTSGLPNSEKARIRQLYAQGKVGRDALLESESSSYH
                     APGTCTFYGTANSNQMLMEVMGLHLPGSAFINPQDPLRDALTAAGARRAVELAGQGKN
                     YIPVGRVVDEKSIVNAIVALLSTGGSTNHTLHLVAMARAAGIVIDWDDFDKLSSVVPL
                     LTRIYPNGEADVNVFQAAGGPGFVIRELLDAGLLHDDVWTVLGQGLRAHCNEPYFESG
                     ALAWRPAPAESGDSKVLRGAANPFAADGGLRLLTGNLGRSVIKVSAVKPQHRVVEAPA
                     LVFDSQEALMDAYRAGKLDRDFIAVVRFQGPRANGMPELHALTPALANLQDAGRRVAL
                     VTDGRMSGASGKVPAAIHVSPEVLAGGPLARVRDGDLIRLDAEAGVLQALVPEAEWDA
                     REFPHADLSASHTGMGRELFAVFRATVGPAEEGAATFGLPPALNMPDETDNPLAVPQE
                     QAYSDEDYLFTAKRKGPTHDPA"
     gene            3520..>3921
                     /gene="eda"
                     /locus_tag="VM406_04155"
     CDS             3520..>3921
                     /gene="eda"
                     /locus_tag="VM406_04155"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HVL75185.1"
                     /translation="MTLLEIMRASPVIPVIAIDDPQQAVPLARALVDGGIRVLEITLR
                     TEHGLGAIRAIAEQVPQAIVGVGTLTQPQEFRAARDAGAVFGVSPGLTATLIEAARSS
                     GLPLLPGVMTPSEVMTAREAGFSQLKLFPAVP"
BASE COUNT          666 a         1271 c         1324 g          660 t
ORIGIN      
        1 aagccttgcg cttgcatgtc ggcccgcgcc tcgtcggcgt tcttgccgcg catgaaggct
       61 tccgcctgcg cgaggcagtt ggccagcagc accgcatgat ggccgggcag cgcgtgcgcg
      121 ggcttgaggg cggcgatgaa atcgaccggc gtggtgtccg ttccctggtg cagcagctgg
      181 aaatacgcgt gctgtccgtt ggtgccgacg tcgccccaga cgatcggacc gctcgcgacc
      241 tcggcctcgt cgccggccag cgtgacccgc ttgccgttgc tctccatgtc gagctgctgc
      301 agataggcgg gaaactggct cagatcctgc aggtaggggg cgatcgagat tgacggccag
      361 cggaagaagt tgcggtacca gatcccgatc atgccgagaa cgaccggcat gttgtgctcc
      421 agtggcgcgg tcctgaaatg ctcgtccatt gcatgggcgc cggcgaggaa ttccgcgaag
      481 cgctcgaagc cgattgccag cgccaccggc aggccgatcg ccgaccacac cgaatagcgg
      541 ccgccgaccc agtcccagaa cggaaacatg ttttccgggt cgatgccgaa gtcgcgtacc
      601 gcgctggtat tcgtcgatac cgcaacgaaa tgccggggca gctcattgac cgggcggcgc
      661 gcggtgaacc aggcgcgcgc ggactcggca ttcatcatcg tctcacgcgt ggtgaaggtc
      721 ttggacgcga cgacgaacag cgtggtctcg ggattgacgc gctggagcac cgcatccagg
      781 tcatgcccgt cgacgttggc gacgaaatgc agggtcagac ccagctgcac aaagggccgc
      841 agcgcgcggc agaccatctc ggggccgtgg tccgagccgc cgatgccgat gttgacgatg
      901 tcggtgatcc gggcgccggt gccgccgacc cattcgccgc tgcgcacgcg ctcggtaaag
      961 gcctggatgc ggtcgagcac cgcatgaacc tcggggatga catcctgtcc atccacgacg
     1021 agcgaccggg aacgcggcat gcgcagcgcg gcgtgcagga cggaacgccg ttccgtcaca
     1081 ttgatcttgg caccggcaaa catggcgtcg cgcctttgct cgacatggcg ctcgcgggcc
     1141 agctgcagca agaggcgcat cgtgtccgca tccacgcggt tcttcgagta atcgagcagt
     1201 agtccggccg cctccagcga gaacgcctcg aagcgccctg gctgccgctc gaacagcgca
     1261 cgcagccccc agtccttggc ggtctcgaag tgttcggcga gggctgtcca cgcggggtag
     1321 tgggtcaacg cgatgtgaga catggtgtgc gcgtcaggag ttgaaaaagg tgttcaacta
     1381 tacaatacga atcggaaaaa ttactacaaa aaatttcgat ccgaatgcac gacacttgat
     1441 tccgctcagc actgctgcgc cgattttcac tcgagatgcg ccggataaag cttgcgcagg
     1501 atcaaatgga ttagcggaag tcggagcgaa ttccggtaac gcaagcatcg acagcatgta
     1561 gtaaaattac agaggttttc gaggagcaaa tcatgcccat acattccact ctggccgcgg
     1621 tcacgcagcg catcaccgag cgcagccgac cattccgcca gtcctatctc gcgcgcatgg
     1681 acgccgcgcg cgtgcagggc gtccatcgcg gcacgctcgc ctgcacgaat ctcgcgcacg
     1741 gcttcgccgc gttcccggcg aacgacaagc tcagactcaa gcagcagcgg gttccttctg
     1801 tcgccatcgt gtccgcctac aacgacatgt tgtcggccca ccagccgttc gagcgtttcc
     1861 cgaagatcat caaggacgcg gtgcgcgagg cgggcggggt cgcccagttc gccggaggcg
     1921 tgccggccat gtgcgacggc gtcacccagg gccagcccgg catggagttg tcgctgtttt
     1981 cgcgcgacac gatcgcgatg ggcaccgcgg tggccttgtc gcacaacatg ttcgacgccg
     2041 ccctctacct cggcgtgtgc gacaagatcg tgcccggcct ggtgatcggc gcgctgcact
     2101 tcggccactt gccggcggtg ttcatcccgg ccggtccgat gacgagcggc ttgccgaatt
     2161 cggagaaggc gagaatccgc cagctttatg cgcagggcaa ggtcggacgg gacgcgctgc
     2221 tggagtccga gtccagctcc tatcacgcgc cgggaacctg caccttctac ggcaccgcca
     2281 acagcaatca gatgctgatg gaggtaatgg gcctgcacct gcccggctcg gccttcatca
     2341 atccgcagga cccgttgcgc gacgcgctaa ccgcggccgg cgcgcgtcgc gcggtcgagc
     2401 ttgccggaca ggggaagaac tacattcccg tgggccgcgt ggtcgatgag aagtccatcg
     2461 tcaatgcaat cgtcgcgctg ctctcgaccg gcggttcgac caaccatacc ttgcacctcg
     2521 tcgcaatggc ccgcgccgcc ggcatcgtga tcgactggga cgacttcgac aagctgtctt
     2581 ccgtggtgcc cctcctgacg cgcatctatc cgaacggcga agcggatgtg aacgtgttcc
     2641 aggctgccgg cggtcccggt ttcgtgatcc gcgaactgct cgatgcgggc ctcttgcatg
     2701 acgacgtgtg gacggtgctg gggcagggct tgcgcgcgca ctgcaacgag ccgtacttcg
     2761 agagtggagc gctcgcgtgg cgtcccgctc cggcggaaag cggcgacagc aaggttctgc
     2821 gcggcgcggc caatccgttc gcggcagacg gggggctgcg gctgttgaca ggcaacctcg
     2881 gccgttcggt gatcaaggtc tcggcagtca agccgcagca ccgagtcgtc gaggcgccgg
     2941 cactggtgtt tgattcccag gaagccctga tggacgccta ccgagccggc aagctggacc
     3001 gtgacttcat tgccgtcgtg cgcttccagg gtccgcgcgc gaacggcatg ccggaactgc
     3061 atgcgctcac gcctgcgctg gcgaacctgc aggacgccgg gcggcgcgtg gccctggtga
     3121 ccgacggccg catgtcgggc gcatccggca aggtgccggc ggcgatccat gtctctccgg
     3181 aggtgctggc cggcggaccg ctggcgcgcg tgcgcgacgg cgacctcatc cgcctggatg
     3241 cggaagccgg cgtgttgcag gcgctggtgc ctgaggccga gtgggacgcg cgcgagttcc
     3301 cgcatgccga cctgtcggcc agccataccg gcatggggcg cgagctgttc gccgtattcc
     3361 gcgccaccgt cgggccggcg gaagagggcg ccgccacctt cggcctgccg ccggccttga
     3421 acatgccgga cgagaccgac aacccgctgg ccgttccgca ggagcaggcc tattcggacg
     3481 aagactatct gttcactgcc aaacgaaagg gaccgaccca tgaccctgct tgagatcatg
     3541 cgcgcctcgc cggtgattcc ggtgattgcg atcgacgatc cgcaacaggc ggtgccgctg
     3601 gcgcgcgcgc tggtggatgg cggcatccgc gtgctggaga tcacgctgcg caccgagcac
     3661 gggctgggag cgatccgggc gatcgccgag caggttccgc aggccatcgt gggcgtcggc
     3721 acgctgactc aaccccagga gttccgggcg gcgcgcgatg cgggggcggt gttcggggtc
     3781 tcgccggggc tgaccgcgac gctcatcgag gccgcgcgca gcagcggcct gccgctgttg
     3841 cccggcgtga tgacgccgtc cgaggtgatg acggcccgcg aggccggctt ttcgcagttg
     3901 aagctgttcc cggccgtgcc g
//
LOCUS       DATLGF010000138         4568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_162156, whole genome shotgun sequence.
ACCESSION   DATLGF010000138 DATLGF010000000
VERSION     DATLGF010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162156"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VM406_04160"
     CDS             <1..553
                     /locus_tag="VM406_04160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVL75186.1"
                     /translation="NPGFALHVAASLAMRLGRTVRILFVGSGDSEAAVRALAAARPGL
                     VEAEFHGHASQTELPALYRSARLFLFPTQADVWGVVANEACAAGLPILVSPHAGVAGE
                     LIVDGENGYVCELDVELWADRAARLLSDEALYRRFAEHSRALVRRYTFEHAATGIVDA
                     CRYAAMRHGAGHIDTSHGTIGRS"
     gene            565..1737
                     /locus_tag="VM406_04165"
     CDS             565..1737
                     /locus_tag="VM406_04165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVL75187.1"
                     /translation="MKVLVAHNAYQQRGGEDMVVDAEVALLREHGHEVEVYRQHNDAL
                     NQMSRAAAAVGTVWSRRTSADIERLCGSFAPDVIHVHNTFPLISPSLYWAAGRHRVPV
                     VQTLHNFRLLCPQAIFLREGRICEDCVGKTPWRAVTRKCYRDSSLQSAVITGMLATHR
                     ALGTYRHQVTRYIALNRFARDKYVEGGLPADRFRIKPNFVASSVRPTSDQRSGGLYVG
                     RLSEEKGVSVLAEAVRALPGASIEVIGSGPLEPLVREVFGPRLLGFRPLDDILVRMAR
                     ARFLVVPSICYENAPRTIVEAFSCGLPVIASRLGSLADMVEDGETGLLFAPGDPADLA
                     RKIAWANAHPEQMARMGRAARLEYERLYTPTRNHELLIDIYEDAIAAVRRQQPCAA"
     gene            1688..2494
                     /locus_tag="VM406_04170"
     CDS             1688..2494
                     /locus_tag="VM406_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HVL75188.1"
                     /translation="MKMQLQPYVDNSPAQREGKPVLEAFIDAMTWSEAISRIAGWAAA
                     RESRYVCICNVHSVVTTTRDVEFKIAVNNADMATPDGAPIAWALRHLGHTGQQRINGP
                     DLMWKYLAEAERRDQSVFFYGSTEATLERLRLTLARKFPLLRIAGCYSPPFRSLSREE
                     DEQIVRMINDSGANVVFVGLGCPKQEKWMADHRGRVQAVMIGVGAAFDYHAGVLKRAP
                     VWWQNHGLEWLFRLGAEPRRLFRRYMVTNTLFVFGFLRQVVVDKLPFSGT"
     gene            complement(2750..3535)
                     /locus_tag="VM406_04175"
     CDS             complement(2750..3535)
                     /locus_tag="VM406_04175"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HVL75189.1"
                     /translation="MMHSHPVFTCSIDDGHPLDLKTAELLEKHGLYATFFIPIRNCEG
                     DSVLGVTGIRQLARRFEVGSHTYDHRYLKTIDVWQAYHQINDGKKRLEDVVGARVNGF
                     CYPGGRYRRRDVELVKACGFTYARTTMNLCFDAGEKPFEIPTTIQFYPHDRAVYLRNF
                     VGSGNWFNRTTGLRLALVHEDWIRRLYAMFDHACRTGGVFHLWGHSKQIEQLAAWRQL
                     DDFLRHVASSIPRTACMTNEQLAARARVDVTAGGAAVVTNGRG"
     gene            complement(3581..>4568)
                     /locus_tag="VM406_04180"
     CDS             complement(3581..>4568)
                     /locus_tag="VM406_04180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVL75190.1"
                     /translation="DFPHVFLKERIRTVKGRYIHNNPDVVTALRRFQPDVVVTDGFNP
                     TQLYAFAYAWFKALPHVPLTDGTLLSEQALSAVHRTVRRVVYVRSHAFLSASLGGQHL
                     YESYGIAPENCFKSYLCIDNDAYLREAHASWKRHDLMFCGRMVPEKGPLFALRIAIET
                     ARRLGRRVSILFVGSGSEDERVREAAAQYSRFVDAHFHGFAKQRELPKLYASARMFLF
                     PTHADVWGVVANEACAAGLPVIVSPHAGVAGELVVHEENGYVCELDAQLWADRCVELL
                     SDEARRARFGRRSLMLVREYSFDNAARGFVDACDHALAAALSNKASKAGEAI"
BASE COUNT          783 a         1513 c         1488 g          784 t
ORIGIN      
        1 gaatccaggc ttcgcgcttc acgtcgcagc atcgctggcg atgcggctgg gccgcacggt
       61 gcgcatcctg ttcgtcggaa gcggcgactc cgaagcggcg gtacgcgcgt tggccgcggc
      121 acggccggga ctggtggagg ccgaattcca cggtcatgca tcgcagaccg agctgccggc
      181 cttgtaccgc tcggcgcgcc tgttcctgtt tccgacccag gccgatgtct ggggcgtggt
      241 cgccaacgaa gcctgcgcgg ccgggctgcc catcctcgta tcgccgcacg ccggcgtagc
      301 gggcgaactg attgtcgatg gggaaaacgg ctatgtctgc gaactcgatg tcgagctgtg
      361 ggccgatcgt gccgctcgcc tgctctcgga cgaggcgctg taccgccgct tcgccgagca
      421 cagccgtgcg ctggtgcgtc gctacacctt cgagcacgcc gccaccggaa tcgtggacgc
      481 ctgccgctac gcggcgatgc gtcacggcgc cggtcacatc gacacgtcgc acggcacgat
      541 cggccgttcc taggggcgca agacatgaaa gtactggtcg ctcacaacgc ctaccagcag
      601 cgcggcgggg aagacatggt cgtcgacgcc gaagtggcgc tgctgcgcga gcacggccat
      661 gaagtcgagg tctatcgtca gcacaatgac gcactgaacc agatgtcgcg tgcggcggct
      721 gccgtcggca ccgtgtggtc gcggcgcacc tcggcagaca tcgaacggct gtgcggcagc
      781 ttcgcgcccg acgtcatcca cgtccacaac acctttccgc tgatctcgcc gtccctgtac
      841 tgggcggccg gacggcatcg cgtgccggtc gtgcagaccc tgcacaactt ccgcctgctg
      901 tgtccgcaag cgatattcct gcgcgagggt cgcatctgcg aggactgtgt cggcaagacg
      961 ccctggcgcg cggtcactcg caagtgctac cgggactcgt ccttgcagtc ggccgtcatc
     1021 acgggcatgc tcgcgaccca ccgtgcgctc gggacctatc gccaccaggt cacgcgctac
     1081 atcgcgctca accgtttcgc ccgggacaaa tacgtggagg gcggattgcc ggcagaccgc
     1141 tttcgcatca agccgaactt cgtcgcctcc agcgtgcgtc caacctcgga ccagcgttcc
     1201 ggcggactgt acgtcggccg cttgtcggag gaaaaaggcg tatcggtgct tgccgaggcc
     1261 gtgcgcgcac tgccaggcgc cagcatcgag gtcatcggca gcgggcctct ggaaccgctg
     1321 gtacgggaag tcttcgggcc gcgccttctt ggtttccggc cgctcgacga catcctcgtg
     1381 cggatggcac gcgcgcgctt cctggtcgtg ccgagcatct gctacgagaa cgcgccgcgc
     1441 accatcgtcg aggccttctc ctgcggcctg ccggtgatcg ccagccgtct cgggtccctg
     1501 gccgacatgg tcgaggacgg tgaaaccggg ctgctgttcg cgcccggaga tcccgcagac
     1561 ctggcacgca agatcgcctg ggccaatgcc caccccgaac agatggcgcg catggggcgc
     1621 gccgcccgtc tcgaatacga acgactctac acgcccacgc gcaaccatga actcctcatc
     1681 gacatctatg aagatgcaat tgcagccgta cgtcgacaac agccctgcgc agcgtgaagg
     1741 caagcccgta ctcgaagcct tcatcgacgc gatgacctgg agcgaggcaa tctccaggat
     1801 cgccgggtgg gccgcggcac gggaatcccg ctatgtctgc atctgcaacg tccactcggt
     1861 ggtcacaacc acgcgcgatg tcgagttcaa gatcgccgtc aacaacgcgg acatggccac
     1921 gccggacggc gcgccgatcg cctgggcgct gcgccatctc ggtcataccg gccagcaacg
     1981 catcaacggg ccggacctga tgtggaagta cctcgcggag gccgagcggc gcgaccagtc
     2041 cgtgttcttc tacggcagca ccgaggccac gctggagcgg ctgcgtctca cgctcgcacg
     2101 caagttcccg ctgctgcgca ttgccggctg ctactcgccc cctttccgct cgctttcgcg
     2161 cgaggaggac gagcagatcg tccgcatgat caacgactcc ggcgccaatg tcgtgttcgt
     2221 cggactgggt tgcccgaagc aggagaaatg gatggcggat caccgcggcc gcgtgcaggc
     2281 ggtgatgatc ggggtcggcg ccgccttcga ttaccacgcc ggcgtgctca agcgcgcgcc
     2341 ggtctggtgg cagaaccacg gactggaatg gctgtttcgc ctgggcgccg agccgcgccg
     2401 cctgttccgg cgctacatgg tgaccaacac gctgttcgtg ttcgggttcc tgcgccaggt
     2461 ggtggtggac aagctgccgt tttcggggac ttgaaccgaa cgcgcgcacc gcccggagcg
     2521 acggcagtct cgccgtggct caaggtccag gcagttgttc accacggcga acacggcgaa
     2581 agtcccaact ccctgtcgtc ccgagcgcag cgagggacct catcgtcccg tgcaccagag
     2641 attcctcgct gcgctcggaa tgactagctt ttccaccgtg gtcgccgtgc tcgccgtggt
     2701 tcagcgttca gccaggtgtg caggcagccc acctcatgcc gggcgggcgc tagccgcgcc
     2761 cgttcgtgac gaccgcggcg ccgccagcag tcacgtcgac gcgggcgcgg gcggccagct
     2821 gttcgttggt catgcaggca gtgcgcggaa tagaagatgc gacgtggcgc aggaaatcgt
     2881 ccagctgccg ccatgcggcg agctgctcga tctgctttga atgtccccac aggtggaaca
     2941 ctccgccggt cctgcaggcg tgatcgaaca tcgcgtacag gcggcggatc cagtcttcgt
     3001 ggaccagcgc caggcgcagt ccggtcgtgc ggttgaacca gttgccggaa ccgacgaaat
     3061 tgcgcaggta gaccgcgcgg tcatgcggat agaactggat cgtcgtcgga atctcgaacg
     3121 gcttttcgcc cgcatcgaag caaagattca tcgtcgtgcg cgcataggtg aagccgcagg
     3181 ccttgacgag ctccacgtca cggcggcgat agcgtccgcc cggatagcaa aagccgttca
     3241 cccttgcccc gacgacatcc tcgagccgct tcttgccgtc gttgatctgg tgatacgcct
     3301 gccacacgtc gatggttttc aggtagcggt ggtcgtaggt gtgcgagccg acctcgaagc
     3361 ggcgcgcaag ctgccggatc ccggtgacgc cgagcaccga atcgccttcg cagttacgga
     3421 tgggaatgaa aaaggttgcg tacaggccgt gcttctcgag cagctctgcg gtcttcagat
     3481 caagcgggtg gccatcgtcg atggaacagg tgaaaacggg gtgggaatgc atcatgtgct
     3541 ccaaaccggg gacaggcgaa acccgctctg cacgttgcgc tcagatggcc tcgccggcct
     3601 tgctcgcctt gttcgacaag gcggcagcga gcgcgtgatc gcaggcatcg acgaaaccgc
     3661 gggcagcatt atcgaagctg tactcgcgca ccagcatcag gctgcgccgc ccgaagcggg
     3721 cacgcctggc ttcgtcgctc agcagttcca cgcagcgatc ggcccaaagc tgggcgtcga
     3781 gctcacagac gtagccattc tcctcgtgaa cgacgagttc tccggccacg ccggcgtgcg
     3841 gggacacgat caccggaagc cccgcggcac aggcttcgtt ggccaccacg ccccagacat
     3901 cggcgtgcgt ggggaacagg aacatgcggg ccgacgcgta cagtttcggc agttcgcgct
     3961 gcttcgcaaa gccgtggaag tgcgcgtcga cgaagcggct gtattgcgcg gccgcctcgc
     4021 gcacgcgttc gtcttcactg cccgatccga cgaacaggat gctcacgcgc cggcccagcc
     4081 tgcgcgcggt ttcaatcgcg atgcgcagcg cgaacaacgg tcccttttca ggcaccatgc
     4141 gtccgcagaa catcaggtcg tgacgcttcc agcttgcgtg cgcttcgcgc aagtaggcgt
     4201 cgttgtcgat gcacaggtag gacttgaagc agttctccgg cgcgatgccg tagctctcgt
     4261 acagatgctg tccgcccagg ctcgccgaca gaaatgcgtg cgagcgcaca tagacgacgc
     4321 ggcgcaccgt ccggtgcacg gcgctcagcg cctgctcgga cagcagtgtg ccgtcggtca
     4381 gcggcacgtg cggcagcgcc ttgaaccacg cgtaggcgaa tgcgtacagc tgggtcggat
     4441 tgaagccgtc ggtgacgacc acgtccggct ggaagcgccg cagggccgtc acgacgtccg
     4501 gattgttatg gatgtagcgg cccttgacgg tacggatgcg ctccttgagg aacacgtgag
     4561 ggaaatcc
//
LOCUS       DATLGF010000139         3207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_209022, whole genome shotgun sequence.
ACCESSION   DATLGF010000139 DATLGF010000000
VERSION     DATLGF010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209022"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VM406_04185"
     CDS             complement(<1..1026)
                     /locus_tag="VM406_04185"
                     /inference="COORDINATES: protein motif:HMM:NF019924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HVL75191.1"
                     /translation="MRPRVLLVASEAIPLIKTGGLADVVASMANALRLQGVEVTLLLP
                     AYPAALAALGCVAEIARLEGLPAGPARLLQGRIAATGVPVLLLENAGFSARSGNPYVD
                     ADGNEYADNAACFAALAHAAVRICAGATPVPVPHVVHANDWHAALIPSLLRAAGIERV
                     GTVLTVHNLAFQGNYPLELGAQVGLPPSMLGPDVAEFWGRLSFLKAGICHADRISIVS
                     HSYAQEVLTPRFGCGLDGALQARREAIVPIPNGVDLDTWNAATDLLIARRFTAADLAS
                     KAWCKRALLRLFDLPVAPHAPLLALGSRITHQKMADVALEALPQVLDRHPRLQVVVLG
                     CGDHAYED"
     gene            1452..3002
                     /locus_tag="VM406_04190"
     CDS             1452..3002
                     /locus_tag="VM406_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HVL75192.1"
                     /translation="MIIYQVYPRSFLDTNDDGIGDLAGIEQRLDYIKRLGADAVWISP
                     FFKSPMKDFGYDVADYRAVDPIFGSMDDFKKLMRRAKSLGLGVFVDMVISHTSEEHPW
                     FRDSQARRIGRDDWYLWADPKPDGSPPNNWVSVFGGTAWRWDAQRRQYYYHSFFSSQP
                     DLNMHNPAVQDAVLAEMEYWLDLGVSGFRFDACNHIFQDLQLRNNPAREDSSAALHPY
                     GFQVHVYDQGRPENLAFLERVRKLLDAYGAYSLAEVGGADPLRLMGQYTEAGRLHSAY
                     SFALMRPDHNADYVRNVIATLEDSVRAPGTPCYAMSNHDKPRVASRWQNGRSFLPAAK
                     MHMALLLAMRGDICLYQGEELGLPEAQVPYERLQDNFGKTFWPKFKGRDGCRTPMPWT
                     DAEHGGFSTAEPWLPVDPAHRAMSVAQQESDPDSMLHFARELIALRKGNPALDQGGIT
                     LLDAPDGVLAFSRRHHGQEVSCLFNLDTSAKRVDAPAFASVLREQNSLVQGGQVHLDV
                     CGFCLLQH"
BASE COUNT          602 a         1079 c         1004 g          522 t
ORIGIN      
        1 atcctcgtaa gcgtggtcgc cgcaaccgag cacgaccact tgcaagcgcg gatgcctgtc
       61 aagaacctgc ggcagcgctt ccagcgccac gtccgccatc ttctggtggg taatgcggct
      121 gcccagcgca agcagcggcg cgtgcggcgc caccgggagg tcgaacaggc gcagcagcgc
      181 gcgcttgcac caggccttgc tggccaggtc ggccgccgtg aaacgccggg cgatgagcag
      241 gtcggtcgcc gcgttccagg tatcgaggtc aacgccgttg ggaatcggca cgattgcttc
      301 gcggcgcgcc tgcagcgcac cgtccaagcc gcagccgaag cgcggcgtca gtacttcctg
      361 cgcataggaa tggctgacga tggaaatgcg gtccgcatgg cagatgccgg ccttcaggaa
      421 actgagcctg ccccagaact cggcgacatc cgggcccagc atcgagggcg gcaggccgac
      481 ttgcgcgccg agttcgagcg gatagttgcc ctggaacgcg aggttgtgca cggtgagcac
      541 ggtgccgacg cgctcgatgc cggccgcgcg cagcagcgaa ggaatcaagg ccgcgtgcca
      601 gtcgttcgca tgcacgacgt gcggcaccgg cacgggcgtc gcgccggcgc agatccggac
      661 cgcagcatgc gccagcgccg cgaagcaggc ggcattgtcc gcatattcgt tgccgtcggc
      721 gtcgacgtaa ggattgccgc tgcgcgcgga aaaccccgca ttctcaagca gcagcaccgg
      781 cacgccggtc gcggcaatgc gtccttgcag aagccgcgca gggccggccg gcaggccctc
      841 cagccgagcg atttcggcaa cgcagccgag cgcggccagc gccgccggat aggcagggag
      901 caggagcgtg acctcgacgc cctgcaggcg caaggcattg gccatggaag cgacaacgtc
      961 ggcgagcccg ccggtcttga tcaaggggat ggcttcggaa gcaacgagta gtacgcgggg
     1021 gcgcaagatg atgacctctt tcggttgaac gcggccgcct tgtcacagtg aacgcacgct
     1081 ccatagccgc gggcggcatg gagaaatttt cgcggtgggg ggtgcaggcg tgtcctgaat
     1141 agtaaatttt ccaaagggcg gcccattacg ccgcgtcaaa tggcaatgca gcaaaactgt
     1201 tgtgctgatg tttatccact cgaacaagtt ccttgtcggc tgaacaaatt ccaccgtcgt
     1261 atgcgcgcga aaatgcattt tttccgcacc tcaagtattg accccacgca aatcatcgct
     1321 accatctgac gcatcagcgt tgcaccgggt aaagccatcc ccctgcatca caccgcttgt
     1381 ttcttcgtca ctccgtttgc ccctcgacgc ctgcaggcgc acggcgggca agcctttcgg
     1441 caagaacccc catgatcatc tatcaggtct acccgcgcag tttcctcgac accaacgatg
     1501 acggcatcgg cgatctcgcc ggcatcgaac agcgcctcga ctacatcaag cgcctcggcg
     1561 cggacgcggt atggatcagt ccgttcttca agtcgccgat gaaggacttc ggctacgacg
     1621 tggccgacta tcgcgccgtc gatccgatct tcggcagcat ggacgacttc aagaagctga
     1681 tgcggcgtgc gaaatcgctc gggctcggcg tcttcgtcga catggtcatt tcgcacacct
     1741 cggaggaaca tccgtggttt cgcgacagcc aggcgcgccg catcgggcgc gacgactggt
     1801 atctgtgggc agatccgaag ccggacggca gcccgccgaa caactgggtg tcggtcttcg
     1861 gcggcaccgc gtggcgctgg gacgcgcaac gccggcagta ttactaccac tcgttcttct
     1921 ccagccagcc ggacctgaac atgcacaacc cggccgtgca ggacgcggtg ctggccgaaa
     1981 tggaatactg gctggacctg ggcgtgtccg gtttccgttt cgacgcgtgc aaccacatct
     2041 tccaggacct gcagctgcgc aacaatccgg cgcgcgaaga cagcagcgcc gcgctgcatc
     2101 catacggctt ccaggtccac gtctatgacc agggccggcc ggagaacctg gccttcctcg
     2161 agcgggtgcg caagttgctc gacgcctacg gcgcctacag ccttgccgaa gtcggcggcg
     2221 ccgatccgct gcggctgatg ggccagtaca ctgaggccgg ccgcctgcat tccgcgtaca
     2281 gcttcgcgct gatgcgcccc gatcacaacg cggactacgt tcgcaacgtg atcgcgacgc
     2341 tggaagacag cgtgcgcgcg cccggcacgc cctgctatgc aatgagcaac catgacaagc
     2401 cgcgcgtggc cagccgctgg caaaacggac gctccttcct gcccgccgcg aagatgcaca
     2461 tggcgctgct gctcgcgatg cgcggcgaca tctgcctgta ccagggcgaa gaactcggcc
     2521 tgcctgaagc gcaagtgccg tacgaacgtc tgcaggacaa tttcggcaag accttctggc
     2581 ccaagttcaa gggccgtgac ggctgccgca cgccgatgcc gtggaccgat gccgagcacg
     2641 gcggcttcag cacggccgag ccatggctgc cggtcgatcc agcccaccgc gcgatgagcg
     2701 tcgcgcagca ggaaagcgat ccggattcca tgctgcattt cgcccgcgaa ctgatcgcgc
     2761 tgcgcaaggg caatccggcg ctggatcagg gcggcatcac gctgctcgat gcccccgacg
     2821 gcgtgctggc cttcagccgc cgccatcacg gacaggaggt aagctgcctg ttcaacctcg
     2881 acaccagcgc aaagcgcgtc gatgccccag ccttcgcgag cgtgctgcgc gaacagaaca
     2941 gcctcgttca aggcggccag gtccatctcg acgtgtgcgg gttctgcctg ctgcaacact
     3001 gaaggtccca ccgcagtatc aatgctgttc aattaagcga atattcgtgc catgtcgttc
     3061 ccgcgcaggc ggaaacccag ttccggcttc cgcggccgcg ccgctctggt catgcacgcc
     3121 gactcccacc cgggcgacgc cgtcacacgg cgcacgcagg ctgcctcatc cgctccgcaa
     3181 cgcgacaaac agccagcccc gctgcgc
//
LOCUS       DATLGF010000140         2321 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_3071,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000140 DATLGF010000000
VERSION     DATLGF010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3071"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="VM406_04195"
     CDS             complement(<1..1292)
                     /locus_tag="VM406_04195"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HVL75193.1"
                     /translation="MAISVFELFKIGIGPSSSHTVGPMRAARQFVAGLKEAGTLMQVA
                     AVKAELYGSLGATGKGHGSDKAVLLGLEGEAPDLVDVDGIDERLAHIRTQRRLFLLGE
                     HEIRFVEGEHLLMYRRQSLPFHPNGMRFTAYAGDGAELASKVYYSVGGGFVVDETAAG
                     AGRIVADNTTVRYPFKTADEMLALCREHRLSISQLMLENESAWRSEHETRAGLLRIWQ
                     VMQECVKRGCEREGVLPGPLKVRRRAAELFRKLSATPEANLRDPLTTLDWVNLYALAV
                     NEENAAGGRVVTAPTNGAAGIIPAVLHYYHRFLPNANEDGVIRFLLTAAAIGILYKEN
                     ASISGAEVGCQGEVGVACSMAAGALAEVMGGTPQQVENAAEIGMEHNLGLTCDPIGGL
                     VQVPCIERNAMGSIKAINAARMALRGDGKHYVSLDKVI"
     gene            complement(1302..>2321)
                     /locus_tag="VM406_04200"
     CDS             complement(1302..>2321)
                     /locus_tag="VM406_04200"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006006.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HVL75194.1"
                     /translation="AGMQVVVVGCDAAGNVDVADLKAKAEKHSKDLAAIMITYPSTHG
                     VFEEAVREVCEIVHAHGGQVYIDGANMNAMVGLCAPGKFGGDVSHLNLHKTFCIPHGG
                     GGPGVGPIGVKAHLAPFLPGHRTLENKELAVCAAPWGSASILPISWTYIKLMGREGLK
                     AASETAILNANYIAKRLAAHYPILYAGEGGLVAHECIVDLRPLKDTGVTVDDVAKRLM
                     DFGFHAPTMSFPVPGTLMIEPTESEAKEELDRFCDAMIAIREEIRTIERGELTAEESP
                     LRFAPHTAADLVGDWDRKYTREQAVFPVRSLRKQKYWPPVGRVDNVYGDRNVVCACPP
                     MADYE"
BASE COUNT          363 a          824 c          719 g          415 t
ORIGIN      
        1 ttgatgacct tgtcgagcga gacgtaatgc ttgccgtcgc cgcgcaaggc cattcgcgcc
       61 gcgttgatcg ccttgatcga acccatcgcg ttgcgctcga tgcaaggcac ctggaccagg
      121 ccgccgatcg gatcgcaggt cagccccagg ttgtgctcca tgccgatctc ggcagcgttc
      181 tcgacctgct gcggcgttcc ccccatgact tcggccagcg cgccggccgc catcgagcag
      241 gccacgccga cctccccctg acagcccacc tcggcccccg atatcgacgc gttttccttg
      301 tacaggatgc cgatcgccgc ggcggtcaag aggaagcgga tcacgccgtc ctcgttcgcg
      361 ttcgggagaa agcgatggta gtaatgcagc acagcgggga tgatgccggc cgcgccattg
      421 gtcggcgcgg tgacgacccg gccgcccgct gcgttttcct cgttgaccgc cagcgcatac
      481 aggttgaccc agtccagcgt cgtcagcgga tcgcgcagat tcgcttcagg cgtggcggag
      541 agcttgcgga acaattcggc cgcgcgccgg cgcaccttca gcggtccggg cagcacgccc
      601 tcgcgctcgc agccgcgctt cacgcattcc tgcatcacct gccagatgcg caagaggccg
      661 gcgcgcgtct cgtgctcgct gcgccacgcg ctttcgttct ccaacatcag ctggctgatc
      721 gatagccggt gttcgcggca tagcgccagc atctcgtccg cggtcttgaa cgggtacctg
      781 acagtagtgt tgtcggcgac gatgcgtccc gcaccggcgg ccgtctcgtc gacgacgaag
      841 ccgccgccga ccgagtagta gaccttgctc gccagttcgg cgccgtcgcc ggcataggcg
      901 gtaaagcgca tgccattcgg atggaacggc agcgactggc gccggtacat cagcagatgc
      961 tcgccttcga cgaatcggat ttcgtgctcg ccgagcaaaa aaaggcgccg ctgcgtgcgg
     1021 atgtgcgcaa gtcgttcgtc gatgccgtcg acatcgacca ggtccggcgc ctcgccttcc
     1081 agcccgagca gcaccgcctt gtcgctgccg tggcccttgc cggtcgcacc gagcgagccg
     1141 tacagctcgg ccttcaccgc ggccacctgc atcagcgtcc cggcctcctt gagcccggcc
     1201 acgaactgcc tggccgcgcg catcgggccg accgtatgcg agctcgacgg cccgatcccg
     1261 atcttgaaca gttcgaagac ggagatcgcc atggcagcgg cctactcgta gtccgccatc
     1321 ggcgggcagg cgcagacgac gttgcggtcg ccgtagacgt tgtccacgcg gccgaccggc
     1381 ggccagtact tctgcttcct cagcgagcgc accgggaaca ccgcctgctc gcgcgtgtac
     1441 ttgcggtccc aatcgccgac cagatcggca gcggtgtgcg gcgcgaaacg cagcgggctt
     1501 tcctcggcgg tgagttcgcc gcgctcgatc gtacggatct cctcgcggat cgcaatcatc
     1561 gcgtcgcaga agcggtccag ctcttccttg gcttcgcttt cggttggctc gatcatcagc
     1621 gtgccgggca ccgggaacga catcgttggc gcgtggaaac cgaagtccat caggcgcttc
     1681 gccacatcgt ccacggtcac gccggtgtcc ttcaacggac gcaggtcgac gatgcactcg
     1741 tgcgcgacca gaccgccctc tcccgcatac aggatcgggt agtgcgcagc cagccgcttc
     1801 gcgatgtagt tcgcgttcag gatcgccgtc tcgctggccg ccttcagtcc ttcgcggccc
     1861 atcagcttga tgtaggtcca gctgatcggc aggatgctcg cgctgcccca gggcgccgcg
     1921 cagactgcca gttccttgtt ctccagcgtg cggtggcccg gcaggaaggg cgccagatgc
     1981 gccttcacgc cgatcggacc gacgccgggc ccgcccccgc cgtgcggaat gcagaaggtc
     2041 ttgtgcaggt tcaggtgcga cacgtcgccg ccgaacttgc ccggggcgca caggccgacc
     2101 atcgcattca tgttcgcgcc gtcgatatac acctgcccgc catgcgcgtg cacgatctcg
     2161 cacacttcgc gcaccgcctc ctcgaacacg ccgtgcgtgg acggataggt gatcatgatg
     2221 gccgccaagt ccttcgaatg cttctcggcc ttggccttca aatctgccac gtcgacgttg
     2281 ccggcagcgt cgcagccgac taccaccacc tgcatgccgg c
//
LOCUS       DATLGF010000141         7268 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_84705,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000141 DATLGF010000000
VERSION     DATLGF010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7268
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84705"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1523)
                     /locus_tag="VM406_04205"
     CDS             complement(<1..1523)
                     /locus_tag="VM406_04205"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HVL75195.1"
                     /translation="MTSGSTVLLECGRTYQGTLDLRGKSDITVRTAGTCGNATISPGR
                     ALSGWSRHSGNVFSVPVEGDVAQVIVDGQPLALAHWPNRPQTWARATSTTATTLTHAM
                     PNADLVGASLVFRPYDWAIEARRITAYAGTTMTLAPTGNINFDGYALSGAVDFYVEGK
                     LWMLDSPGEWAVSEGRLHVWTPDGQSPEGRVWVSGPRDGIDAVATRAITIQDVNIYGA
                     VNGINAVNASALRVVNVHIANSSGNGILNSGGSALAVERSSIRNSRHDAIAVKWGGGG
                     EVIRNNVIDASGTVGMPTNVHAAINLVAGTGSLVQNNTVTRSGYIGIRAFRNAAVSGN
                     TIDQACLVLTDCGGIYTDAPDRAPLNTRIENNTISRVAPDQRLAWGIFLGEYANGVTI
                     AGNTITDSCNGIEILNGFSNRISNNVFARNTQAHIQMVEFGGAPVVANNVVSGNTFTT
                     TGRQEMYRLSSELGTSAIARFGSYAGNSYVSNASIFANYNGEALSFAQWRARTGQDG"
     gene            2107..2241
                     /locus_tag="VM406_04210"
     CDS             2107..2241
                     /locus_tag="VM406_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75196.1"
                     /translation="MIDDYTAHSRLRVLNRVAEGLQRRLGKQATKPEAAAPVAQKEAA
                     "
     gene            complement(2238..2900)
                     /locus_tag="VM406_04215"
     CDS             complement(2238..2900)
                     /locus_tag="VM406_04215"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVL75197.1"
                     /translation="MSTRSKKPTVDFRQAQESLRADLRQGMLDAATRLLDEEGPSALT
                     VRRVADAVNCSTTLLYSLFGGKDGLANELYLEGFARLKREFEDARAAAEKKKSGRDGL
                     APLLLLAKVYHQYAKRNPSYYMVMFGDAIAGFVPPVASRKQAWESLAVLIDTFDAAMK
                     DGTLPPSNPTAAARLLWAAMHGAISLELKGYYLKSERAAELFDAAVDAVLLALQQPAR
                     SR"
     gene            3027..3998
                     /locus_tag="VM406_04220"
     CDS             3027..3998
                     /locus_tag="VM406_04220"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HVL75198.1"
                     /translation="MSNPRLPAALQNLALPVIASPMFIASGPALVTAQCKAGIVGSFP
                     ALNARPAEMLDKWLTDIQAELAAYRAANPGAKIGPIAVNQIVHHSNDRLEHDVEVCVK
                     HQIPIIISSLRAPPQEMLDAVHSYGGIVLHDVISIRHAKKALEAGVDGLILVAAGAGG
                     HAGMLSPFALVGEVRKFFDGPIALSGAIATGDAVLAAEAMGADFAYIGSRWLATQEAN
                     VDDAYREAIIESTAADVIYTNLFTGVHGNYLRKSIEAAGLDPENLPVADKTKMNFGSG
                     GNTKAKAWRDIWGAGQGVGLMDAVLTVPEVMEKLKAEYMAARRRLAL"
     gene            complement(4137..5600)
                     /locus_tag="VM406_04225"
     CDS             complement(4137..5600)
                     /locus_tag="VM406_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HVL75199.1"
                     /translation="MKTACKFIVPALLLAGCAAVQPPPPYQRPDLGLTPADTFAAAPG
                     EPTQPTSADLLWWRRFDDPAMTQWVEQALAGNLEVAAAFERVEQAQALLRRASAERGP
                     VLGARGQIGVNRRSGGAGRTSGTSGSAGTTTGGVQTNGATAGAGLSIEWNADLWGGLR
                     QAEASAAASLLRSEDLAQAARLATAGTAARGYIEWREAQNEWQVLEQTLRLREDMVRL
                     VRVRVDVGLSPAIDLTRAQADLAAVQAERDAAAGRARQAEVALHVLAGRRPSATTPLP
                     QADARIPDLAGVVPVPRPVDLLRLRPDVRAAERALIAAYADVGVARAALYPTLRLPGD
                     LLVTAAGLGTGSIVQSVTASLGAILDATLIDGGRRAADVDAARSRAREAALVYERTVL
                     EALEQVESALIARETITSELQARRAAAETATQALEQARAMYTEGLAGFADVLEAQRTW
                     LANRLEFTRGEAAAARAAIASFEAIGVVDPGQMRAPA"
     gene            complement(5597..>7268)
                     /locus_tag="VM406_04230"
     CDS             complement(5597..>7268)
                     /locus_tag="VM406_04230"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVL75200.1"
                     /translation="SLVPAIAARLSFTREPGRIQTRMAALGHSLGERYERVLRRLLGV
                     PWLVVLACLVVVGIAALLFPRIGEELVPEEDRGVLRIVMNGPDGVGLSYMDRQVELAE
                     QVVAQAGAGAAIRNVFTITGGWDPNRGVVIVRLHHWDERDLDQGELAARLRRPLSEIP
                     GAQVRVAGGNSLGIRGADGSLQFSLTGPDHESIAKAADAFARVLEENLAPLQDIRVEY
                     RPTQPQITLALDRRRAADLGVSIDAIRAALRALVDGDEIAELSIEDRTVPVIVESTAG
                     SVRGPQDLMSLQVRAAGERLVPLSQFVTLTEQGVPAELERHGLRRAVSMSAQPADGYT
                     LRAAVDDVRALASEHLPDGINLIFRGEAATLEETARGVWITFGIALLVVFLVLVAQFE
                     SLSSAAVVMITVPLALCAAVFALLLTGTTLNIYSQIGLLLLVGVTAKNGILMVEFANQ
                     LRDRGRSVEQATAEAASVRLRPITMTIASTVMGALPLIIGSGPGAESRAAIGWVIFGG
                     LGLAVVFTLFLTPVVYLLLSRHAKPRSAQGRRLDEEIRAQSVTAEKSP"
BASE COUNT         1162 a         2459 c         2460 g         1187 t
ORIGIN      
        1 tctccatcct gtccggtgcg cgcgcgccat tgcgcgaacg acagcgcctc gccgttgtag
       61 ttggcgaaga tcgacgcatt gctcacgtag ctgttgccgg cgtagctgcc gaagcgcgca
      121 atcgcggacg tgccgagttc gctgctcagc cggtacatct cctggcgccc ggtggtcgtg
      181 aaggtattgc cgctgacgac gttgttcgcc accaccggcg cgccgccgaa ctcgaccatc
      241 tggatgtgcg cctgcgtgtt gcgtgcaaag acgttgttgc tgatgcggtt gctgaagccg
      301 ttgaggatct cgataccgtt gcagctgtcg gtgatcgtgt tgccggcgat cgtcaccccg
      361 ttcgcatatt cgccgaggaa gatgccccag gccagccgct ggtccggcgc gacccggctg
      421 atcgtattgt tctcgatgcg ggtgttcagc ggcgcgcgat ccggcgcatc ggtgtagatg
      481 ccgccgcagt cggtcagcac caggcaggcc tggtcgatgg tgttgccgga caccgccgca
      541 ttgcggaacg cgcggatgcc gatatagccc gagcgcgtga cggtgttgtt ctgcacgagc
      601 gatccggtgc cggcgaccag gttgatcgcc gcatgcacgt tggtcggcat gccgaccgtg
      661 ccggatgcgt cgatcacatt gttgcggatg acttcgccgc cgccgcccca tttgaccgcg
      721 atcgcgtcat ggcgcgagtt gcggatgctg ctgcgctcga cagcgagcgc gctgccgccg
      781 gagttcagga tgccgttgcc ggacgaattc gcgatatgta cgttgacgac gcgcagcgcg
      841 gatgcattga ccgcattgat gccgttgacc gcgccgtaga tgttcacgtc ctggatcgtg
      901 atcgcgcgcg tggcgaccgc atcgatgccg tcgcgcggac cggaaaccca gacgcgtcct
      961 tccggcgatt ggccgtccgg cgtccagaca tggagccggc cttcgctcac cgcccattct
     1021 ccgggcgaat caagcatcca cagcttgccc tcgacataga aatcgaccgc gcccgacagc
     1081 gcatagccgt cgaagttgat gttcccggtc ggggccagcg tcatcgtcgt gcccgcgtag
     1141 gcggtgatgc ggcgcgcctc gatcgcccag tcgtaaggac gaaacacgag gcttgcgccg
     1201 accaggtccg cattcggcat tgcgtgcgta agcgtggtgg cggtcgtcga cgtcgcacgc
     1261 gcccaggtct gcggccggtt cggccagtgc gccagcgcga gcggctgacc gtcgacgatc
     1321 acctgcgcga catcgccttc caccggtacc gaaaacacat tgccgctgtg ccggctccag
     1381 ccggacagcg cgcgtcccgg gctgatcgtc gcgttgccgc aggtacccgc ggtgcgcacg
     1441 gtgatgtcgg acttgccgcg caagtccagc gtgccctggt aggtgcggcc gcattcgagc
     1501 aggaccgtgc tgccgcttgt caccgtcggc agcggcagcg gcggctgcgc attcgcgggc
     1561 ggagcgagtg aggccggaaa actcgcgtcg gcagatgcat gcacgctggt ggaggcgtcg
     1621 gatgatgcgc cgcttccgcc gcaggctgcg agcgccgcga tcagggttaa ggtcgccgcg
     1681 cgatgcgctg gttcagcgac gcggcgcccg ggtgagacac cacgacctgt ctgtttggca
     1741 ttcggtgaat tcatgttctt cttgcacgca gccgcagagg gtgcggcctg ttggatcgga
     1801 actcgattga atcaccggca atggaattga ggaaatgccg gcctgataat ttcaggcggg
     1861 aaaattgtta cgttcagtga gttttgtaag gggatgggca tgcggcgcga cttgcctgac
     1921 gtgcttctaa aaaatatttc tgaatggaac aaaactcgct gcgcccgcat ctaaccgcca
     1981 tcttgtcctt cccgtctctt tcaacctcag tcgcaatcgg tcggaaacat gaagtggtat
     2041 tacgaatggc gcctcaacag ggtgcgtgca acaatttctg cgcttgaaca agaaacaagg
     2101 ctgcgattga tcgacgacta caccgcgcat tcacgactgc gggtgctgaa ccgcgtcgcc
     2161 gagggattgc agcgccggct tggcaagcag gcgaccaagc cggaggcggc tgcgcccgtc
     2221 gcgcagaagg aagcggccta acgcgaacgc gcgggttgct gcaacgcgag cagcaccgca
     2281 tcgaccgccg catcgaacag ctccgccgcg cgctcgctct tcaggtagta gcccttaagt
     2341 tcaaggctga tcgcgccgtg catcgccgcc cataaaaggc gtgctgccgc ggtcggattg
     2401 gacggcggca gcgtgccgtc cttcatcgcc gcatcgaagg tgtcgatcag caccgcgagc
     2461 gactcccacg cctgcttgcg cgatgcgacc ggcggcacga agccggcgat cgcatcgccg
     2521 aacatcacca tgtagtaact ggggttgcgc ttcgcatact ggtgatagac cttggccagc
     2581 aagagcagcg gcgcaaggcc gtcgcggccg ctcttcttct tttcggccgc ggcgcgcgcg
     2641 tcctcgaact cgcgcttcag gcgcgcgaag ccttccaggt agagctcgtt ggccaggccg
     2701 tccttgccgc cgaacagcga atagagcagc gtcgtcgagc agttcaccgc gtcggcgacg
     2761 cggcgcaccg tgagcgcgga cgggccttcc tcgtcgagca ggcgcgtggc ggcatccagc
     2821 atgccctggc gcagatcggc gcgcagactc tcctgcgcct ggcggaaatc caccgtcggt
     2881 ttcttggaac gggtactcaa ttcggccttg ctgataacgt tgttttttct gataacatca
     2941 taaacagaac cacgaaaaaa acgcaaatcg cgcgcccgct tcgcgcagct tgcacgagac
     3001 caacgagaca tctccaagga gccatcatga gcaatcctcg cctgccggcc gccctccaga
     3061 atcttgcact gccggttatc gcctctccga tgttcatcgc cagcggcccc gcgctggtga
     3121 ccgcccagtg caaggccggc atcgtcggct cgtttccggc gctcaatgcg cgtccggcag
     3181 agatgctgga caagtggctg accgacatcc aggccgaact ggctgcgtac cgcgcggcca
     3241 atcccggcgc aaagatcggg ccgatcgcgg tgaaccagat cgtgcaccat tcgaacgacc
     3301 gcctcgaaca cgatgtcgag gtctgcgtga agcaccagat cccgatcatc atctccagcc
     3361 tgcgcgcgcc gccgcaggag atgctggacg cggtgcacag ctatggcggc atcgtgctgc
     3421 acgacgtgat ttcgatccgc catgcgaaga aggcgctgga ggctggcgtg gacggcctga
     3481 tcctggtcgc ggccggtgcc ggcggccatg ccggcatgtt gtcgccgttc gccctggtcg
     3541 gcgaagtgcg caagttcttc gatggcccga ttgcgctgtc cggcgcgatc gccaccggcg
     3601 acgcggtgct ggcggccgag gcgatgggcg cggatttcgc gtacatcggc tcgcgctggc
     3661 tcgcgacgca ggaagccaac gtcgacgatg cgtaccgcga ggcgatcatc gaatcgaccg
     3721 ccgcggacgt gatctacacg aacctgttta ccggcgtgca cggcaattac ctgcgcaagt
     3781 cgatcgaggc ggccggtctc gatccggaga atctgccggt ggccgacaag accaagatga
     3841 acttcggctc gggtggcaac accaaggcca aggcctggcg cgacatctgg ggtgcgggcc
     3901 agggcgtggg cctgatggat gcggtgctga cggtgccgga agtgatggag aagctcaagg
     3961 ccgagtacat ggcggcgcgg cgcaggctgg cgttgtgaag cgctgacgcg gttttatgcg
     4021 gccgagctgg gtccccgcct gtacagggac gacatggcgg ggtaatcacc cgatgcagga
     4081 tgtgcctcga cgcagagcgc gcaagagttt cggcgattcc atgttcgcct cccccctcac
     4141 gccggtgctc gcatctgacc cggatcgacg accccgatcg cctcgaagct cgcgattgcg
     4201 gcgcgcgcgg ctgcggcttc gccgcgggtg aactcgagcc ggttggccag ccaggtgcgc
     4261 tgggcctcga gtacgtccgc aaagcccgcc agtccttccg tgtacatcgc gcgcgcctgc
     4321 tcgagcgcct gggtcgccgt ttcggcagcg gcgcggcggg cctgcagttc cgatgtgatc
     4381 gtctccctgg cgatcagcgc gctttccacc tgttccagcg cttccagcac cgtgcgttca
     4441 tagaccagcg cggcttcgcg cgcgcgcgag cgagccgcgt cgacatcggc ggcgcgcctt
     4501 ccgccatcga tcagcgtcgc atcgaggatc gcgcccaacg aagccgtcac gctttgcacg
     4561 atgctgccgg tgccgagacc ggcggcggtc accagcaggt cgccgggcag gcgcagggtc
     4621 ggatacagcg cggcgcgcgc aacgccgaca tccgcatagg cggcgatcag ggcacgctcg
     4681 gcggcgcgca cgtccggccg caggcgtagc aggtcgaccg ggcgcggcac cggcaccacg
     4741 ccggcaaggt cggggatgcg cgcgtcggcc tgcggcagcg gcgtcgtggc cgaggggcgc
     4801 cggccggcca gcacgtgcag cgccacttcc gcctggcgcg cccgacccgc agccgcatcg
     4861 cgctcggcct gcaccgccgc caggtcggcc tgcgcgcgcg tgaggtcgat cgccggtgac
     4921 aggccgacgt ccacgcgtac ccgcaccagg cgcaccatgt cttcgcgcaa gcgcagcgtc
     4981 tgctccagca cctgccattc gttctgcgcc tcgcgccatt cgatgtagcc gcgcgcggcg
     5041 gtgccggcgg tcgccaggcg cgcggcctgg gcgaggtctt cgctgcgcag cagcgaggcg
     5101 gcggccgagg cttcggcctg gcgcaggccg ccccatagat cggcattcca ttcgatcgac
     5161 aggccggcgc ccgcggtggc gccattggtc tgcacgccgc cggtggtcgt gcctgcgctg
     5221 ccggacgtgc cgctggtgcg cccggcgccg ccgctgcgcc tgttcacgcc gatctggccg
     5281 cgcgcgccca gcaccgggcc gcgttcggcg ctggcgcggc gcagcagcgc ctgcgcctgc
     5341 tcgacccgct cgaacgcggc cgccacttcc aggttgccgg cgagcgcctg ttcgacccat
     5401 tgcgtcatcg ccgggtcgtc gaagcggcgc caccagagca gatcggcgga cgtcggctgc
     5461 gtcggctccc ccggcgcggc ggcgaaggtg tcggccgggg tgaggccgag gtcgggacgc
     5521 tggtagggcg ggggcggttg taccgccgcg cagcctgcca gcagcagggc gggaacgatg
     5581 aacttgcaag cggtcttcat gggctcttct ctgcggtaac ggattgggcg cggatttcct
     5641 cgtccaggcg ccggccttgc gcgctgcgcg gcttcgcatg cctggataac aacaggtaga
     5701 ccacgggcgt gaggaaaagt gtgaacacga ccgccaggcc gagaccgccg aagatcaccc
     5761 agccgatcgc cgcgcgcgat tccgcgcccg gcccgctgcc gatgatcagg ggcaaggcgc
     5821 ccatcacggt ggaggcgatc gtcatcgtga tcggccgcag gcgcacgctc gcggcttctg
     5881 cggtcgcctg ctcgacgctg cggccgcggt cgcgcagctg gttcgcgaac tcgaccatca
     5941 ggatgccgtt cttcgccgtc accccgacca ggagcagcag gccgatctgg ctgtagatgt
     6001 tgagcgtggt gccggtcagc agcagcgcaa ataccgccgc gcacagggcc agcggcaccg
     6061 tgatcatcac caccgccgcc gaggacaggc tctcgaactg cgcgaccagc accaggaaca
     6121 ccacgagcaa agcgataccg aacgtgatcc agacgccgcg cgcggtctct tccagcgtgg
     6181 cggcctcgcc gcgaaagatc aggttgatgc cgtccggcag gtgctcggac gcgagcgcgc
     6241 gcacgtcgtc gaccgcggcg cgcagcgtgt agccgtcggc cggctgggcg ctcatcgaca
     6301 ccgcgcggcg caggccgtgg cgttcgagtt cggccggcac gccttgctcg gtcagcgtga
     6361 cgaactgcga cagcggcacc aaccgctcgc cggcggcgcg cacctgcagc gacatcaggt
     6421 cctgcgggcc gcgcacgctg ccggcagtgg attcgacgat caccggcacc gtgcggtcct
     6481 cgatcgacag ctcggcgatc tcgtcgccgt cgaccagcgc gcgcagcgcg gcgcggatcg
     6541 catcgatcga cacgccgagg tcggcagcac gccggcggtc cagcgccagc gtgatctgtg
     6601 gctgggtcgg ccggtattcg acgcggatgt cctgcaaggg ggcgaggttt tcctccagca
     6661 cgcgggcgaa tgcatcggcg gccttggcga tgctttcatg atcggggcca gtcagcgaga
     6721 actgcaggct gccgtccgcg ccgcgtatgc cgaggctgtt gcccccggcc acgcgcacct
     6781 gagcgccggg aatctccgaa agcgggcgac gcaaacgcgc ggccagctcg ccctggtcca
     6841 ggtcgcgctc gtcccagtga tgcaggcgca cgatgaccac gccgcggttt gggtcccagc
     6901 cgccggtgat cgtaaacacg ttgcggatgg ctgcgcccgc gccggcctgc gcgaccacct
     6961 gctcggcaag ctcgacctgg cgatccatgt aggagagccc gaccccgtcc ggaccgttca
     7021 tcacgatgcg cagcacgccg cggtcttcct ccggcaccag ttcctcgccg atgcgcggaa
     7081 acaggagcgc cgcgatgcca accaccacca ggcaggccag caccaccagc cacggcacgc
     7141 cgagcaggcg gcgcagcacg cgctcgtaac gctcgcccag gctgtggcct agcgccgcca
     7201 tgcgcgtctg tatccgaccc ggctcgcgcg taaacgacaa gcgcgccgcg atcgccggca
     7261 ccagcgac
//
LOCUS       DATLGF010000142        19809 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_52897,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000142 DATLGF010000000
VERSION     DATLGF010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 19809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19809
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52897"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /locus_tag="VM406_04235"
     CDS             complement(<1..1332)
                     /locus_tag="VM406_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HVL75201.1"
                     /translation="MRSAPTAQIVDLVERRVLSLTPPQALFFSFFALSLVGTLLLKLP
                     AATTSPISWMQALFTATSAATVTGLVVVDTGTQFTLTGQLILLALLQLGGLGFMSFGV
                     FIISLARSRLNLGQRAVMREALNQSGSGDMRRLLRRMFVFTVVIELVGTLLLATKWVP
                     ELGWGRGLYHSFFHAISAFNNGGFVLDSDSLMSEVGSPIVNIVITGLFISGGIGFVVV
                     ADMMEKQRFSDYTLHTKLMLFGTAILSVVGMLVLFILEYGNPDTLGGLASLSDQLWAA
                     WFASVTPRSAGFNTIDTGAMTEASALFTMVLMFIGGGAGSTAGGIKLTTFIVILLTAR
                     AFLLGHDRPVVFGRSLDYGIIIRALTVSVIWVIAVILGTFILTLTESARFIDIAFEVT
                     SAGCTVGLSRGITPDLSPIGQATVIGLMLIGRAGPLALAFVLVHRYPSRIQY"
     gene            complement(1329..1979)
                     /locus_tag="VM406_04240"
     CDS             complement(1329..1979)
                     /locus_tag="VM406_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HVL75202.1"
                     /translation="MKNTITVIGLGIFGSTIALELTRLGHDVLGIDINPKRVADIADR
                     ITQAVVADARDERVLNELGVPDSDVVVVAIGEDIEANILASLLADSMPKPRVWAKALN
                     HNHHQILSKIGTDYIIHPEHEMGLRVARTLIYPEVVDYISLGDDLFISEVRASERLIG
                     KKLEHLHLDEYGVQCLMVKHHGLAMSPPQSEYLLELGDQIVLFGTLGQLRKIAKYL"
     gene            2419..3402
                     /locus_tag="VM406_04245"
     CDS             2419..3402
                     /locus_tag="VM406_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVL75203.1"
                     /translation="MIKQFRRTAFSLCASALLVAPALAQSPAQNYPNQTVRIVVPYAP
                     GGFNDTIARVMGKKLQDAWGQPVIADNRPGGGTILGLTHAAKAPADGYTLVVVGFPTV
                     ANQFLYKRLPYDTNKDFTPVMLGAYSPNLLVVREDSPIKSVKDFVEMAKAKPGRLNYA
                     TAGNGTSNHLTMEYFKSMAGIDLVQVPYKGSAPMITDMLGGQIDIMFDNAPHVMPHVK
                     AGKLRALAITSAKRSPLLPDVPTVAELGYPGFEVSVWYGFAAPAGTPPAIVAKLNQEM
                     NKALQADDVKKIFADSGVEPLGGSVQQFDAFFKAQTERWGKVIKEANVTAD"
     gene            3425..6430
                     /locus_tag="VM406_04250"
     CDS             3425..6430
                     /locus_tag="VM406_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVL75204.1"
                     /translation="MDMRAAGAAQSKVAEPFVGRPMQRIEDVSLLTGRGQYGDDVGVK
                     PGTLYAAIVRSPHAHAILGAVDTSAAERAPGVRAVLTGKDIGEWSRPFVVGVKAPMEM
                     WALARERVRFVGDPVAVVVAESRYLAEDAADLVKVEYTPLPAVTTVEDAIRADAPVLH
                     EGVGSNIVSDRSFRYGDPETAFANADHVIELHAEYPRNSCTPIEGSVVVAEYLSDEEG
                     YDASSNFMGPFSLHTVMAMALRVPGSKLRHRTFRDSGGSFGVKHAVFQYVVLMCLASR
                     KARAPVKWVEDRLEHLVALTSATGRVSTIRAAVQADGRITALSYDQFDDCGGYLRAPE
                     PATFYRMHGCLTGAYAIPNLAVHNRVVLTNKTPAGLVRGFGGPQVYFQLERLMQRISV
                     TLGIDPIELYRRNFIAADAFPYSAPAGALIDSGNYQLALELVEREGGLAELYARRERA
                     RAEGRLFGIGFAAIVEPSISNMGYISTVLPKEARSKAGPKNGAIASATLAIDPLGSVS
                     VVIASAPAGQGHRTVCAQVVADVFGISPELVAVNVDLDTQKDAWSVAAGNYSSRFAGA
                     VAGTVHLAACKMRDKLARIAAPQLGCEPGDVVFEGGHIYAKDKPQPSLPFARMAASPH
                     WAPGLLPEGEEPGLRETAFWTAEQLTAPDEDDRVNTSAAYGFAFDVCAVEIDRNTGRV
                     RIDKYVTTHDAGKILNPALADGQIRGAFAQALGAALMEEFRYGEDGSFQSGTFADYLV
                     PTTCEVPEPTIIHMETPSPFTPLGAKGLGEGNSMSTPPCIANAVADALGVADVQLPLT
                     PSKVMALIGMDDRPPSKPRAVEAPAAPRGDKKGGKSLAARGEVRLAASPEAVFGVLLD
                     PVALAKVIPGCNALEQVGENRYRADVTVGVGMIKARYAAEVALSELDPPRSLRLAGSG
                     LSAVGSAAGSGLVTLEPAEGGTLLRYDYAADVSGKVAAVGSRMLEGAAKIVLNQLFEQ
                     LGREAGGGGAAPVRLSWWQRLLALLGVQR"
     gene            6427..7245
                     /locus_tag="VM406_04255"
     CDS             6427..7245
                     /locus_tag="VM406_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HVL75205.1"
                     /translation="MKPQAFDYVRADSADEAVALLADSGSEARILAGGQSLMAVLNMR
                     LAQPSLLIDISRTPDLNYVRARDGVLAIGAAATQASVEWRKTLAQEVPLLAQAFPHIS
                     HFQVRNRGTVCGSVAHADPSAELPLCLLVLGGQVVLRRRKGRRVLAADDFFQGMLMTA
                     READELVEEVRYPLAAAGHRYAFNEFSIRHGDFAIVSVAAAVGPQDIRFAVGGVADRP
                     RVTSLPLLTGADLDTAINDFAWELEARDDHHASAVFRRHLVRRLGREVIEKAMA"
     gene            7242..7763
                     /locus_tag="VM406_04260"
     CDS             7242..7763
                     /locus_tag="VM406_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HVL75206.1"
                     /translation="MKKHVKGELMQVRLVLNGQERSGACEPRTLLSDFLRDTLGATGT
                     HVGCEHGVCGACTVQIDGVAGRSCLTLAVQADGRRIDTVEGLAGDKEGLSDLQEAFRR
                     NHALQCGFCTAGILMSCDDYLKRKPDPTEAEVRDMLSGHLCRCTGYTNIVKAVLETAA
                     SRRERAAAVLTND"
     gene            7776..9329
                     /locus_tag="VM406_04265"
     CDS             7776..9329
                     /locus_tag="VM406_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVL75207.1"
                     /translation="MLDLGRTFLQSVERSPNALAIIDGERRFSYAQWYEQIQRTAQGL
                     RSNGIGKGDHIVVILQNRYEMATLHWACQFLGVIVTPLNWRAKPEEVDYCATDAQARA
                     IVFEAISAESVAASPVAQGLPRIAVGDAPGGTLAFEQLQQQAPAAPILEAGSDDYSLM
                     LYTSGTTGKPKGVPRRHRHERAAALAHVAQNLYRRGERTLGVMPLYHTMGVRSLLAMA
                     LVDGLFVCVPKFDSGLALRCIAEEKITCLYLVPTLYHDMLAWREQHGADISSVTKLGF
                     AGAPMHDALLQRLNEVFKPELFVNHYGSSEVYTFSIDQDATKKPGSAGRAGINTRIRV
                     VKLNAQTPDDLAAVGEEGQIIADLSGDEAFEAYWNRPDANAKSIRDGWYFTGDTGYFD
                     EDGDLFVSGRVDDMIISGGENISPVDIESVLSLHPAVDDVAVAGLKDERWGQRVVAFV
                     KPKGEIDAAALDAHCKHSDLLNFKRPREYVFVREIPKSPVGKVLRRKLVAGEYEPAGK
                     EETDKEGSA"
     gene            9586..10446
                     /locus_tag="VM406_04270"
     CDS             9586..10446
                     /locus_tag="VM406_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVL75208.1"
                     /translation="MTTALRIAQGNFGRVALLDMDAPLVTHAHSQCHVILKASGADSA
                     FYVSEERLPVTDRTAILVNAWEPHYFHSEPDFPRTVYLVLYIEPKWLAALHDPLRLSA
                     HPGFFPRPCVDITPHIRKLADDLIGDMLSLIPIAQDKLETALFDLMLAISEQHSAWRE
                     IDGVVRPQGLQLPDSRIQRAITYIRDNLGRPLDADALAASAHLSRAQFFVRFRRATGM
                     TPNVFINTLRTETACRRLACQDERINMGRLSEDLGFSDQAHFTRFIRGHLGVTPSEYR
                     RVVQVYGDAA"
     gene            complement(10938..11870)
                     /locus_tag="VM406_04275"
     CDS             complement(10938..11870)
                     /locus_tag="VM406_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HVL75209.1"
                     /translation="MFFLHPPKLREAEVFTTMPDRFRKKGVSTIWADANRGGAQIDSF
                     LEGPVFDRAGNLYVTDIPFGRIFRISPAGEWELVAEYDGEPNGMKFLNDEELVIADYK
                     NGLVRLDIKTGQVRPFLDRRNTERFKGVNDLVFDSRGNLYFTDQGQTGMHDPSGRVYR
                     LTPDGKLDLLISNAPSPNGLVFSPDEKVLFVAMTRGNCIWRAPLLPDGSVGKVGQFFT
                     SYGPSGPDGLAMDEAGQLIVANPGLGWAWVLNGRGEPVEILRSTKGTSLTNVAFGGNG
                     RRTAFFTESVSGSVLYADMPNMGRAIFQGRASVV"
     gene            complement(11887..12822)
                     /locus_tag="VM406_04280"
     CDS             complement(11887..12822)
                     /locus_tag="VM406_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVL75210.1"
                     /translation="MFGAVTLSQAAHAAYPEKPVRIVVPYAPGGAVDVVARKIAQKLT
                     EQTKQSFFVENRAGATGTIGTNFVARAPADGYTLLAIDNTYSVLPYVFKNLPWNHATG
                     LVPITVSMFAPVVAVVPGDSQFKDLPSLVAHARANPKKLTYGSGGNGSAPHFTGEAFQ
                     LVTGTDLMHVPYKGAGEAMTGLISGQIDMIFVSTPSAGSHVKGGKARALAISGTARST
                     SLPAVPTFAEAGLQNFNIMNWSGLAAPAGTPDAVVNFLHSEVKKALASPDVKEFLENM
                     GGQAGGMAPADFARLIKDETSRWEEVARKANIERQ"
     gene            complement(12941..13879)
                     /locus_tag="VM406_04285"
     CDS             complement(12941..13879)
                     /locus_tag="VM406_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HVL75211.1"
                     /translation="MSASAPSRPVILVTGADLAPQAVEILEKFELVYAGKTPDEDGLV
                     ELCARHQPVAILVRYGKISARVMDASKKLRVISKHGSGIDTIDSAAAAERGIAVKAAA
                     GANAVAVAEHAWALIFACAKDVVKLNTRMHGGHWDKATHKSLELCGKTLGLIGMGAIG
                     RKTAEVGVALGMKVLAFDPYASAVPEGVIMEELDTVMREAHVLSLHCPLTESNRNMIS
                     RDAIGKMRDGAILVNTARGGLVDEEAVLEALQSGKLRAAGLDSFQREPLVGGHLFTNV
                     DNAILSPHIGGVTADAYIGMGCGAARNILAVLNGEI"
     gene            complement(13876..14577)
                     /locus_tag="VM406_04290"
     CDS             complement(13876..14577)
                     /locus_tag="VM406_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HVL75212.1"
                     /translation="MTEQTKAFPEIRRDFARVSSELVAQAAGFQAAILADVNERRGAL
                     HGRIAAVSPSMKLAGSAVTVEVRPGDNLMIHAAMAIAHPGDVLVIDGKGDLSSALMGA
                     IMMNQAKAMGLAGVVVDGAVRDILELQELGFPVFAAGTNPNGPTKYIPGRVNWDISIG
                     GVAIHPGDLVVGDADGVVVIEREKVASLMAPAARKVADENKRIQGILRGEQLRPGWLD
                     GALAAAGLLKQGETL"
     gene            14769..15683
                     /locus_tag="VM406_04295"
     CDS             14769..15683
                     /locus_tag="VM406_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL75213.1"
                     /translation="MAFDIDLRDLRYFEAIAELQHVGRAAEKLHRTQPALTSAIRRLE
                     AACGAPLFEKAGRGIRLSEAGHVLLKWAQRMRYDMEDARREIAEFGQGLSGQVRIGIV
                     PTAAQFILPAAARELLHEAPNVKLKTVVGLVDTMKPLLQSGELDLMVGTESAKDAQFA
                     SELLAEDQIVVAAYKKHELFSRAVKLEDLTRYRWVLQPPGAPTRDWLDHTFERKGFPR
                     PQVQVEASMLFILPALIAETGLLSFVSRHHLGQTKGTTLREIRLPATTMRRRLVVTYR
                     KDGYLSPAASRMIAILAATAKQNLSPDR"
     gene            15981..17105
                     /locus_tag="VM406_04300"
     CDS             15981..17105
                     /locus_tag="VM406_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVL75214.1"
                     /translation="MKWIRRIPAHIINCPSSLFAGAAHFGTRCNKGSDEGAWNGAHPL
                     ITTETLMNTIGRVFLAAALSATMTSAAFAQAEYPNKPIRLIVPFPPGGGTDIVARVVA
                     NKLQEELKWTIVVENRPGAGGTIGLDVAAKAPADGYTIVMGQTSNLTVSPALYPRLTY
                     NPVRDFAPVTTVATAPLVLVTRTDSPFKTVADVISAAKAKPGEINFASPGNGTVAHLT
                     GELFQKAANVKLEHIPYKGTAQAMTDVLGGKVELYMSSVPSAMSQIKSGKIRPIVVTS
                     AKRSSDLPNVPTMHESGYKDFDAFTWFGLLVPAGTPAPVISRLNTEVNKVLARKDVQE
                     RISAEGGNVQGSTPEAFAALIKEEIPRWAVVVKESGATID"
     gene            17260..17709
                     /locus_tag="VM406_04305"
     CDS             17260..17709
                     /locus_tag="VM406_04305"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HVL75215.1"
                     /translation="MTHRNYKDILGGLILVVLGLFVAYYSTQHYQMGTLTRMGPGAFP
                     RALGYLLAGLGVLILVPAFFRSGTRMIFDWRPFLACATSILVFAITVKYVGMIPAIVL
                     LVVTAALADDKLSPKGIIALAIAASILGVVIFSYGFEMSIPLVKWGG"
     gene            17714..19261
                     /locus_tag="VM406_04310"
     CDS             17714..19261
                     /locus_tag="VM406_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HVL75216.1"
                     /translation="MDLANLAGNMALGLSVAFSYTTLLYCFIGVLLGTFVGVLPGIGS
                     LAAIALLLPITFHLDPTSAVVMLAGVYYGSTYGGSTASILLNLPGTPSSAVVCLDGYP
                     MAQQGRAGVALFMTTIASFVGGSLGIIILMLFAPAIGEFALQFGPAEYFAMMLLGLVA
                     ASTISAGSPIKGIAMVVLGVLIGIIGTDVNSGTQRLTFDTPELADGIHLVAIAMGIFG
                     VAEVIASIRNVRHRKVDNKSITFRSMVPTRDDWRRSWGPMARGTAIGSFFGALPGTGG
                     TIASFMSYAVEKKVSKDPERFGKGAIEGIMGPETANNACDQTAFIPTLTLGIPGSVVM
                     ALIIGALMIHGIAPGPSLMVENPDLFWGLIMSFWIGNIMLLVINIPLIGVWVRVLSVP
                     HHVLYPIILMFICMGAYSVNNSNFDVYLVLAFGVIGYLMRVLEFPPAPLLLGLVLGPM
                     MEEYLRRALLLSRGNFSTFLESPISATLICLTGVLLLWSLVALVMPRRPKVVRSVAPP
                     AAELSTE"
BASE COUNT         3629 a         6338 c         6295 g         3547 t
ORIGIN      
        1 gtattggatc cgcgacggat agcggtgcac cagcacgaag gccagcgcga gcggtccggc
       61 gcggccgatc agcatcagcc cgatcaccgt ggcctggccg atcgggctca ggtcgggcgt
      121 gatgccgcgc gaaaggccga ccgtgcagcc ggccgaggtc acttcgaatg cgatgtcgat
      181 gaagcgggcc gattcggtca gcgtcaggat gaaggtgccg agaatgacgg cgatcaccca
      241 gatcaccgaa accgtcaacg cgcggatgat gatgccgtag tccaggctgc ggccgaacac
      301 gacgggcctg tcgtggccga gcaggaaggc gcgcgcggtc aggaggatga cgatgaaggt
      361 agtgagcttg atgccgcccg cggtcgagcc cgcgccgccg ccaatgaaca tcagcaccat
      421 cgtgaagagc gcgctggcct ccgtcatcgc accggtgtct atcgtgttga agccagccga
      481 gcgcggtgta accgaggcga accacgcggc ccacagctgg tccgacaggc tggccagtcc
      541 gcccagtgtg tcggggttgc cgtattccag tatgaacagc accagcatgc cgaccacgct
      601 gagaatggcc gttccgaaca gcatcagctt ggtgtgcagc gtgtaatccg aaaaacgctg
      661 cttctccatc atgtcggcca ccaccacgaa gccgatgccg cctgagatga acagaccggt
      721 gatcacgata ttgacgatcg ggctgccaac ctcggacatc agactgtccg aatcgagcac
      781 gaagccgcca ttgttgaacg ccgagattgc gtggaagaag ctgtgataca ggccgcgtcc
      841 ccagccgagc tccggtaccc atttcgtcgc cagcagcagc gtgccgacca gttcgatgac
      901 gaccgtgaag acaaacatgc ggcgcaagag ccgccgcatg tcgccgctgc cgctctggtt
      961 cagcgcctcg cgcatcaccg cgcgctggcc aaggttcagg cggctgcggg cgagtgaaat
     1021 gatgaatacg ccgaagctca tgaagcccag gccgcccagt tgcagcagcg cgagcaggat
     1081 cagctggccg gtcagcgtga actgggtgcc ggtgtcgacc acgaccaggc cggtgacggt
     1141 cgcggcggac gtggcggtaa acagcgcctg catccagctg attggcgagg tggtggccgc
     1201 cggcaacttc agcagcagcg tgcccaccag cgacagcgcg aagaacgaga aaaacagcgc
     1261 ctgcggcggc gtcaggctca ggacccggcg ctcgaccagg tcgacgatct gcgcagtggg
     1321 tgccgatctc acaggtattt agcgatcttg cgcagttgcc cgagcgtgcc gaacaggacg
     1381 atctggtcgc ccagttccag caggtattcc gactgcggcg gcgacatcgc cagcccatgg
     1441 tgcttgacca tcaggcattg cacgccgtat tcgtccaggt gcagatgctc gagcttcttg
     1501 ccgatcaggc gctccgaggc gcgcacttcg gaaatgaaca ggtcgtcgcc gaggctgatg
     1561 tagtcgacca cctccggata aatcagcgtg cgcgccacgc gcaggcccat ttcatgctcg
     1621 ggatggatga tgtagtcggt gccgatcttc gacaggatct ggtggtggtt gtggttgagc
     1681 gccttcgccc agacccgggg cttgggcatg ctgtcggcca gcagcgaggc gaggatgttg
     1741 gcctcgatgt cctcgccgat cgccaccacg accacgtccg aatcaggcac accgagttca
     1801 ttgaggaccc gttcgtcgcg cgcgtcggcg accaccgcct gggtgatgcg gtcggcgatg
     1861 tcggccaccc gcttgggatt gatgtcgatg cccagtacgt catgaccgag ccgggtgagt
     1921 tcgagcgcga tggtcgagcc gaagattccc aggccgatga cggtgatggt gtttttcatg
     1981 tctgcccgct ctttctgcgc acaagaaccg tctttgcgcc gcaacccgca atgataatcg
     2041 accggcagcg ggcatgcctg cccggttgcc tccccggcgc gatcccgttt tcgggctggt
     2101 tcggagcgag ccaggcatca gcggcggtga taaaggtgag gttgctgact tgcatgttcg
     2161 cccgctctca tgcgctcgat cggaacgtag acggaatgcc ggccccgcgc gccgcgggat
     2221 ttcaatatca gttatacatc ccatcagaaa acattatttg tattgatgga tgaccggtcc
     2281 ttatacttca gcggacacaa gaagaagacc cacccgcgcg acgggaacga agagggggag
     2341 acggcctgtc ggacatgctt gtccctgtgt ctccttctcc acccgccccg gaaattcgaa
     2401 ccaaggattg gagaccacat gatcaagcag tttcgccgca ccgccttctc cctttgcgcc
     2461 agcgcccttc tggttgcacc ggccctggcg cagtcgcctg cgcagaacta cccgaaccag
     2521 acggtgcgca tcgtcgtgcc gtatgcgccg ggcggtttca acgacacgat tgcccgcgtg
     2581 atgggcaaga agctgcagga tgcatggggg cagccggtga tcgcggacaa ccggccgggc
     2641 ggaggaacga ttcttggcct cacccatgcg gccaaggcgc cggccgacgg ctatacgctc
     2701 gtggtcgtgg gctttccgac ggttgccaac cagtttcttt acaagcgcct gccctacgac
     2761 acgaacaagg atttcacgcc ggtgatgctg ggcgcgtact cgccgaacct gctagtggtg
     2821 agggaagact cgccgatcaa gtcggtgaag gactttgtcg agatggccaa ggccaagccg
     2881 ggcaggctga actacgcgac cgccggcaac ggcacgtcca atcacctgac gatggagtac
     2941 ttcaagagca tggctggcat cgacctggtg caggtgccgt acaagggcag cgcgccgatg
     3001 atcaccgaca tgctcggcgg ccagatcgac atcatgttcg acaacgcgcc gcacgtgatg
     3061 ccgcacgtca aggccggcaa gctgcgtgcg ttggcgatta ccagcgcgaa gcgctcgccg
     3121 ctgctgccgg atgtgcccac cgtcgccgaa ctcggctatc cgggcttcga agtctcggtg
     3181 tggtacggct ttgccgcgcc ggccggcacg ccgcccgcga tcgtcgccaa gctcaatcag
     3241 gaaatgaaca aggcgctgca ggccgacgac gtgaagaaga tcttcgccga ctccggcgtg
     3301 gagccgctgg gcggcagcgt gcagcagttc gacgccttct tcaaggcgca gaccgaacgc
     3361 tggggcaagg tgatcaagga agccaacgtc acagcggact gagtctcacc gcaaaggaac
     3421 gcagatggat atgagagccg ccggggcggc acagagcaag gtcgcggagc cgtttgtcgg
     3481 ccgtccgatg cagcgcatcg aagacgtgtc gctgctgacc ggccgcggcc agtatggcga
     3541 cgatgtcgga gtcaagcccg gcacgctgta cgcagccatc gtgcgttccc cgcatgccca
     3601 cgcaatcctc ggcgcggtcg acaccagcgc ggcggaacgc gcgccgggcg tgcgcgccgt
     3661 gctcaccggc aaggatatcg gcgagtggtc gcgccccttc gtggtcggcg tgaaagcgcc
     3721 gatggagatg tgggcgctgg cgcgcgagcg cgtgcgtttc gtcggcgacc cggtcgccgt
     3781 ggtggtggcc gaatcgcgct acctggccga agacgcggcc gatctggtga aggtcgagta
     3841 cacgccgctg cccgcggtga cgaccgtcga agatgcgatc cgtgccgatg cgccggtgct
     3901 gcacgaaggc gtcggctcga acatcgtcag cgatcgcagc tttcgctacg gcgatccgga
     3961 aaccgcattc gccaatgccg accatgtgat cgaactgcac gcggagtacc cgcgcaattc
     4021 ctgcaccccg atcgagggtt cggtcgtggt cgccgaatac ctgtccgatg aagaaggcta
     4081 cgacgcctcc tccaacttca tggggccgtt ctcgctgcac accgtgatgg cgatggcgct
     4141 gcgcgtaccc ggcagcaagc tgcgccaccg caccttccgc gattccggcg gcagcttcgg
     4201 cgtcaagcac gcggtgttcc agtacgtggt gctgatgtgc ctggcttcac gcaaggcgcg
     4261 cgcgccggtg aagtgggtcg aggacaggct cgaacacttg gtcgcgctca cctctgccac
     4321 cgggcgcgtg tccaccatcc gcgcggcggt gcaggccgac ggccgcatca ccgcgctgtc
     4381 ctacgaccag ttcgacgact gcggcgggta tctgcgcgcg ccggagccgg cgaccttcta
     4441 ccgcatgcac ggctgcctga ccggcgccta tgcgattccc aatctcgcgg tacataaccg
     4501 cgtggtgctg accaacaaga cgcccgccgg gctggtgcgc ggcttcggcg gcccgcaggt
     4561 gtatttccag ctcgagcgcc tgatgcagcg catctcggta acgctgggca tcgatccgat
     4621 cgagctgtac cgccgcaatt tcattgcggc cgacgcgttt ccgtacagcg cgcccgccgg
     4681 cgcgctgatc gattccggca actaccagct tgcgctggaa ctcgtggagc gggagggcgg
     4741 cctggccgag ctgtatgcgc gccgcgagcg cgcgcgcgcc gaagggcggc tgttcggcat
     4801 cggctttgcc gcgatcgtcg agccctcgat ctccaacatg ggctacatct ccaccgtgct
     4861 gcccaaggaa gcccgttcca aggccgggcc gaagaacggc gcgatcgcct cggccacgct
     4921 ggccatcgat ccgctcggca gcgtgagcgt ggtgattgcc tcggcgccgg ccgggcaggg
     4981 gcaccgcacc gtctgcgcgc aggtggtggc cgatgtgttc ggcatttcgc ccgagctggt
     5041 ggcggtgaac gtggatctcg atacgcagaa ggatgcgtgg tcggtcgcgg ccggcaacta
     5101 ttccagccgc ttcgccggcg cggtggccgg caccgtgcac ctggccgcct gcaagatgcg
     5161 cgacaagctg gcccgcattg ccgcgccaca gctcggctgc gaaccgggcg atgtggtgtt
     5221 cgaaggcggc catatctacg caaaggacaa accgcaaccg tcattgccgt tcgcgcgcat
     5281 ggcggcaagc ccgcactggg cgcccggcct gctgcccgag ggcgaggagc ccggcctgcg
     5341 cgagaccgcg ttctggaccg ccgagcagct gaccgcgccg gacgaagacg accgcgtcaa
     5401 tacctcggcc gcctatggtt tcgcattcga cgtctgcgcg gtcgagatcg accgcaatac
     5461 cgggcgcgtg cgcatcgaca aatacgtgac gacgcatgac gccggcaaga tcctcaaccc
     5521 cgcgctcgcc gacggccaga tccgcggcgc gttcgcgcag gccctcggtg ccgcgctgat
     5581 ggaggaattc cgctacggcg aggatggcag tttccagtcc ggcacctttg ccgactatct
     5641 ggtgccgacc acctgcgaag tgccggagcc gaccatcatc cacatggaga cgccgtcgcc
     5701 gttcacgccg ctgggcgcga agggcctggg ggagggcaac agcatgagca cgccgccctg
     5761 catcgccaat gcggtggccg acgcgctcgg cgtggccgac gtgcagctgc cgctgacgcc
     5821 gtcgaaggtg atggcgctga tcggcatgga cgaccggccg ccgtcgaagc cgcgcgcggt
     5881 agaggcgccg gccgcgccga ggggcgacaa gaagggcggc aaatcgctcg cggcccgcgg
     5941 cgaggtgcgg ctggccgcat cgccggaagc ggtgtttggc gtgctgctcg atccggtcgc
     6001 actggccaag gtgatccccg gctgcaatgc gctcgagcag gtcggcgaga accgttaccg
     6061 cgccgacgtg accgtcggcg tgggcatgat caaggcgcgc tatgcggccg aggtggcctt
     6121 gtccgaactc gatccgccgc gcagcctgcg cctggccggc tcgggcctgt ccgccgtggg
     6181 cagtgccgcc ggaagcggac tggtgacgct ggagccggcc gagggcggca cgctgctgcg
     6241 ctacgactat gcagccgacg tctccggcaa ggtggccgcg gtcggcagcc gcatgctcga
     6301 aggcgcggcg aagatcgtcc tgaaccagtt gttcgaacag cttgggcgcg aggcgggcgg
     6361 cggcggggcc gcaccggtgc gcctgagctg gtggcagcgc ctgctagcgc tattgggagt
     6421 gcagcgatga agccgcaggc tttcgattac gtccgggccg acagcgccga tgaggcggtg
     6481 gcgctgctgg ccgattccgg ctccgaggcg cgcatcctgg ccggcggcca gtcgctgatg
     6541 gcggtgctca acatgcgcct ggcgcagccc agcctgttga tcgatatctc gcgcacaccg
     6601 gacctgaact acgtgcgcgc gcgggacggc gtgctcgcca tcggcgcggc cgccacgcag
     6661 gccagcgtgg aatggcggaa gacgctcgcg caggaggtac cgctgcttgc gcaggccttc
     6721 ccgcatatct cgcatttcca ggtacgcaac cgtgggactg tctgcggctc ggtcgcccat
     6781 gccgatccga gcgccgaact gccgctgtgc ctgctcgtgc tcggcggaca ggtggtgctg
     6841 cgccgtcgca agggccgccg cgttctggcc gccgacgatt tcttccaggg catgctgatg
     6901 accgcgcgcg aagccgacga actggtcgag gaagtccgct atccgctggc cgcggccggc
     6961 caccgctacg cgttcaacga gttctcgatc cgccacggcg acttcgcgat cgtttcggtg
     7021 gccgcggcgg tcggtccgca ggacatccgc ttcgccgtcg gcggcgtggc cgaccgtccg
     7081 cgcgtgacct cgctgccgct gctcacgggc gcggacctcg ataccgcaat caacgatttc
     7141 gcctgggagc tggaggcgcg cgacgaccat cacgcgtccg ccgtgttccg gcgccacctg
     7201 gtgcgccgcc tcgggcgcga agtcatcgaa aaggcaatgg catgaaaaag catgtgaagg
     7261 gcgaactgat gcaggtgcgc ttggtgctca acggccagga gcgcagcggc gcctgcgagc
     7321 cgcgcacctt gttgtcggac ttcctgcgtg acacgctcgg cgcaaccggc acccacgtcg
     7381 gctgcgagca cggcgtctgc ggcgcctgca ccgtgcagat cgacggcgtg gccggtcgct
     7441 cgtgcctgac gctggccgtg caggccgacg ggcggcgcat cgatacggtc gaagggctgg
     7501 cgggcgacaa ggaaggcctg tccgacttgc aggaggcgtt tcgcaggaac catgcgctgc
     7561 agtgcggctt ctgcaccgcc ggcatcctga tgtcctgcga cgactacctg aagcgcaagc
     7621 ccgaccccac cgaggcggag gtgcgcgaca tgctgtccgg ccacctgtgc cgctgcaccg
     7681 gttacaccaa catcgtcaag gccgtgctcg aaaccgccgc ttcgcggcgc gagcgtgccg
     7741 ccgcggtact cacgaacgac tagaaggagc aagccatgct ggacctggga cggactttcc
     7801 tgcagagcgt ggagcgcagc ccgaatgcgc tcgccatcat cgacggcgag cgccgtttca
     7861 gctatgcgca gtggtatgag cagatccagc gcaccgcgca ggggctgcgc agcaacggca
     7921 ttggcaaggg cgaccatatc gtcgtgatcc tgcagaaccg ctacgaaatg gcgaccctgc
     7981 actgggcctg ccagttcctt ggcgtgatcg tcacgccgct gaactggcgc gccaagccgg
     8041 aggaagtcga ctactgcgca accgatgcgc aggcgcgcgc gatcgtgttc gaggcgatca
     8101 gcgccgagtc ggtcgcagcc agcccggtcg cgcagggcct gccgcgcatt gcggtgggag
     8161 atgcaccggg cggcacgctg gccttcgagc agctgcagca gcaggcgccc gccgcgccta
     8221 tcctcgaagc cggcagcgac gactactcgc tgatgctgta cacctccggc accaccggca
     8281 agcccaaggg cgttccccgc cgccaccgcc acgaacgcgc ggccgcgctc gcgcatgtcg
     8341 cacagaacct gtaccgccgc ggcgagcgca cgctcggcgt gatgccgctg taccacacga
     8401 tgggcgtgcg ctcgctgctg gcgatggcgc tggttgacgg cctgttcgtc tgcgtgccga
     8461 agttcgattc cggcctcgca ctgcgctgca ttgccgaaga aaagattacc tgcctgtacc
     8521 tggtgccgac gctgtaccac gacatgctgg cgtggcgcga gcagcatggc gccgacatct
     8581 cctcggtcac caagctcggc ttcgccggcg cgccgatgca tgacgcgctg ctgcagcgcc
     8641 tgaacgaggt cttcaagccc gaactgttcg tcaaccacta cggctcgtcc gaggtctata
     8701 cgttcagcat cgaccaggac gcgacgaaga agccgggcag cgccggccgc gccgggatca
     8761 acacccgcat ccgcgtggtg aagctcaacg cccagacgcc ggatgatctg gccgcagtcg
     8821 gcgaggaagg gcagatcatt gcggatctca gcggcgacga agccttcgag gcctactgga
     8881 accggccgga tgcgaacgcc aaatcgatcc gcgacggctg gtacttcacc ggcgacaccg
     8941 gctatttcga cgaggacggc gacctgttcg tgtccggccg cgtcgatgac atgatcatca
     9001 gcggcggcga gaacatctcg ccggtggaca tcgaatcggt gctgtcgctg catccggcgg
     9061 tggacgacgt ggcggtggcc ggcctgaagg acgagcgctg gggccagcgc gtggtggctt
     9121 tcgtcaagcc caagggcgag atcgacgccg ccgcgctgga cgcgcactgc aagcactcgg
     9181 acctgctgaa cttcaagcgc ccgcgggagt atgtgttcgt gcgcgaaatt ccgaagtcgc
     9241 cggtgggcaa ggtgttgcgg cgcaagctgg tggctggcga atatgagccg gcgggcaagg
     9301 aagagacgga caaggaaggc agcgcctgag caacttgctg cggcgcacgc tgacccggaa
     9361 aaaaggggca ttgccggcca tgccgttcag cccaggcctc gttattcccc gcgtcgcccc
     9421 ggcgaaagcc ggggcccagc gtcttccgtc cacgtcttga cgcccctgag tcccggcttt
     9481 tgccgggatg accgagacca tggatgcgca tccgacggga atcaggacag agcccgacac
     9541 cggcggcggg gatcgaagaa cttttcggaa tgttcggaga agacgatgac aacagcgctc
     9601 agaatcgcac aagggaattt cggcagggtc gccttgctcg acatggacgc gccgctggtc
     9661 acgcacgcgc attcccagtg ccatgtgatc ctgaaagcca gcggcgccga ttcggctttc
     9721 tacgtcagtg aggagcgcct tccggtgacg gaccgcaccg cgatcctcgt caatgcctgg
     9781 gagccgcatt acttccacag cgagccggat tttccgcgca ccgtctacct ggtgctctac
     9841 atcgaaccga agtggctggc cgcactgcac gacccattgc gcctgtccgc gcatcccggc
     9901 ttcttcccgc gcccctgcgt cgacatcaca ccgcacatcc gcaagctcgc cgacgatctg
     9961 atcggcgaca tgctgtcctt gattccgatc gcacaggaca agctggagac ggccttgttc
    10021 gacctgatgc tggcaatcag cgaacagcat tcagcctggc gcgagatcga cggggtcgtg
    10081 cggccgcaag ggctgcagct gcctgactcc cgcatccaga gagcgatcac ctacatccgg
    10141 gacaatctcg gccgtccgct ggacgcggac gcgctcgccg cctctgccca cctgtcacgc
    10201 gcccagttct tcgtgcgctt ccgtcgcgcg accgggatga cgccaaacgt gttcatcaac
    10261 acgttgcgca ccgaaaccgc atgccggcgc ctggcctgcc aggacgagcg catcaacatg
    10321 gggcggctct cggaagatct cggtttctcg gatcaggcgc acttcacgcg cttcatccgc
    10381 gggcatctcg gggttacgcc aagtgagtat cggcgggtgg tgcaggtgta tggggatgcg
    10441 gcgtgaaaaa tggtgcggga cggggaatac ctccacgtcg tgtgcgatgg catcgatacc
    10501 actgtctcct gttccccctg atgtggcatc aggtaacgtg aacgtggtca tcttgcactt
    10561 ggcagggatt gcgcggcact tgaacgctgg gtgaagggcc gcactcgacc caacccgatg
    10621 tggccggctc agtaaagcag ccactcgccc ggcaaatatc gaatggttca ctggtatcta
    10681 caaagctgac ggcagttcag agagcaactc gaggcagcaa aaagaagaaa atccacctgg
    10741 atcttcgcct aatcttcacc gctgctgaca tagtccctgc tgagaagaga agtggcatgt
    10801 ctgacgagct atacctgaga taccgcggcg gcaattaatg cttggccatt cgagatccgc
    10861 cgtcacatcg atctcctgcg ctatgcaatc ttgggaggta tccaagggac tttctcaaca
    10921 atccaggtcg cagacgtcta aacgacactc gcccgccctt ggaagatcgc acggcccatg
    10981 ttcggcatgt ccgcgtacag gacagaaccc gacacggatt cagtgaaaaa ggccgtgcgg
    11041 cgtccgttcc ctccaaaagc cacattggtt aacgaagtgc ctttggtgct acgcaggatc
    11101 tcgaccggct caccacgccc gttcagtacc catgcccagc ccaagccggg gttggcaaca
    11161 atgagctgac ctgcttcgtc catcgccaac ccgtccgggc cactcgggcc atacgaggta
    11221 aagaactgac cgaccttgcc cactgacccg tcgggaagca ggggcgcgcg ccagatgcag
    11281 ttcccgcggg tcatcgcgac gaacaagacc ttttcatcgg gcgagaagac gagcccattc
    11341 ggactcggtg cattggatat caagaggtcc agcttgccgt ccggcgtgag ccggtacacg
    11401 cgccccgagg ggtcgtgcat gccggtttgc ccttgatcgg taaagtagag atttccgcgc
    11461 gaatcaaaga ccaggtcgtt gacgcccttg aagcgttcag tattgcggcg atcgaggaag
    11521 ggccggacct gaccggtctt gatgtccagg cgcaccaggc cgttcttgta atccgcgatc
    11581 acgagttcct catcattgag gaatttcatg ccgttcggct cgccgtcata ctcggcaacc
    11641 agttcccatt cgccggccgg gctgatccgg aagatccgcc cgaacgggat atcggtcacg
    11701 tacaggttgc cggcccggtc gaataccggg ccttcgagaa atgagtcgat ctgcgcccca
    11761 ccgcgatttg cgtccgccca tatcgtgctc acgcccttct tccggaatct gtccggcatg
    11821 gtcgtgaaga cttcggcttc gcgcagcttt ggcgggtgca gaaagaacat gtcgactccg
    11881 ttcagattat tgacgctcga tgtttgcctt cctggcgact tcttcccaac ggctcgtttc
    11941 gtccttgatc aggcgcgcga aatcggccgg ggccatgccg cctgcctgcc cgcccatgtt
    12001 ttccaggaac tccttgacgt ccggagaagc aagggctttc ttgacttcgc tgtgcaggaa
    12061 gttcaccact gcgtcgggtg tgccggcggg cgctgcaagg ccagaccagt tcatgatatt
    12121 gaagttctgc agccctgcct ccgcaaacgt cggtaccgca ggcaacgagg tcgagcgcgc
    12181 agtaccgctg atggccagcg cacgcgcctt gccgcccttg acatgggaac cggccgaagg
    12241 cgtcgacacg aaaatcatat cgatctggcc ggagatgagg ccggtcattg cctcgcccgc
    12301 gcccttatac ggtacgtgca tgagatccgt tccggtcacc agctggaacg cttctccggt
    12361 gaaatgcggt gcactgccgt tgccgcccga tccataggtc agcttcttgg gattggcgcg
    12421 ggcatgcgca accaggctag gcaggtcctt gaactgggaa tcgccgggca ccaccgcgac
    12481 caccggggcg aacatgctga ccgtgatcgg aaccaagccc gtcgcgtgat tccatggcag
    12541 gttcttgaag acatacggga gcaccgaata cgtattatcg atggccagca gggtgtagcc
    12601 atcggcgggg gcacgggcca caaaattggt gccgatcgta ccggttgcgc cagccctgtt
    12661 ctcgacgaag aaggattgct tggtctgctc ggtcagtttc tgggcaatct tgcgggcgac
    12721 cacgtcgacg gcgccgccgg gtgcgtacgg gaccacgata cgaaccggct tctcgggata
    12781 ggcagcatgc gcggcttgcg aaagcgtgac ggctccgaac aacgctacga gcaggcgagt
    12841 gaatttcatc tttgtctcct tctggtaaga gccgctttca gcggcacggc aatgcaggga
    12901 tgtggccgcg gacggtatgt tatacagtcc gcacacgacg ctaaatctcg ccgttgagca
    12961 cggccaggat attgcgggcc gcaccacagc ccattccgat ataggcgtcg gcagtgacgc
    13021 caccgatatg cggactgagg atggcgttgt ccacattggt gaaaaggtgc ccaccgacga
    13081 gcggctccct ctggaagctg tcgaggccgg cggcgcgcag tttgcccgat tgaagcgctt
    13141 ccagcacagc ctcttcatcg accaggccgc cgcgcgcggt attgaccagg atcgcaccgt
    13201 cgcgcatctt gccgattgcg tcacggctga tcatgttcct gttcgactcg gtcaacgggc
    13261 agtgcagcga caacacgtgc gcttccctca tgactgtgtc gagttcctcc atgatcacgc
    13321 cctccggcac ggcgctcgca tacggatcaa aggccagcac cttcatcccg agcgcaacgc
    13381 cgacttccgc agtcttccgg ccgatggcgc ccatgccaat caggcccagg gtcttgccgc
    13441 aaagctccag gcttttgtgc gtggctttgt cccaatgacc gccgtgcatg cgcgtattga
    13501 gtttcaccac gtccttggca caggcaaaga tcaacgccca ggcatgctcg gccactgcta
    13561 ctgcgttggc gcccgccgcc gccttcaccg caatgccgcg ctcggcggca gctgcggagt
    13621 cgatggtgtc gatgccggat ccgtgcttcg agatcacacg cagcttcttg ctggcgtcca
    13681 tcacgcgcgc agaaatcttc ccgtagcgca cgaggatggc aacgggctga tgccgcgcgc
    13741 acagttcgac caggccgtct tcgtcgggag tcttccctgc gtagaccagc tcgaattttt
    13801 ccaggatctc caccgcttgc ggggccagat cagcgccggt gacgagaatt accggacgcg
    13861 agggtgcgga cgcgctcata gggtttctcc ttgcttgagc aggcccgcag cggcaagcgc
    13921 accgtccagc cagcccggtc gcagctgttc gccgcgcaga atgccctgga tccgcttgtt
    13981 ctcatccgca actttcctgg cagccggcgc catcagcgaa gcgactttct cgcgctcgat
    14041 gacgaccacg ccgtcggcat cgccgaccac caggtcaccg gggtggatcg ccactccgcc
    14101 tatcgagatg tcccagttca cgcggccggg aatgtacttg gtcgggccgt tcgggttggt
    14161 gcccgctgca aagaccggaa agcccagctc ctgcagttca aggatgtcgc gaaccgctcc
    14221 atcgacgaca acgcccgcaa ggcccatcgc cttggcctgg ttcatcatga tcgcgcccat
    14281 cagtgccgaa ctcagatcgc ccttgccgtc gatgaccagc acgtcgccgg gatgtgcgat
    14341 tgccatcgcc gcatgaatca tcaggttgtc accggggcgt acctcgaccg tgacggccga
    14401 gccggccagc ttcatcgaag gagacacggc ggcaatccga ccgtgcaggg caccccggcg
    14461 ctcattgacg tcggcaagga tggcggcctg gaacccggcg gcctgggcca ccagttctga
    14521 cgaaacacgc gcgaagtcgc ggcggatttc ggggaatgct tttgtctgct ctgtcatggg
    14581 gcttctgcgg aaaaaggagt gatcaactgg catcggcgcg tcagaaagac gcccgccaac
    14641 tgagatgctg ctgtcgccgc ccgcagggtg aaatcggcgt gggccggtac caggggagga
    14701 ggcagtgggc cgagtatgtg tgacataatc aaccgcgtct atttcatgtt tcttcacgat
    14761 ccataaatat ggcttttgat attgatctgc gggatctgcg ctacttcgaa gcgattgcgg
    14821 agctgcaaca cgtcgggcgg gcggccgaga aactgcaccg aacgcaaccg gcgctgacca
    14881 gcgccatccg ccggctggaa gcggcctgcg gcgcgccgct cttcgagaaa gccggccgcg
    14941 gcatccgcct ctcggaagcc ggccatgtgt tgctcaagtg ggcgcagcgc atgcgctacg
    15001 acatggagga tgcgcgccgg gaaatcgcag agttcgggca gggtctttcg gggcaggtac
    15061 gcattggcat cgtgccgacc gctgcgcaat tcatcctccc cgcggccgct agagagcttc
    15121 tgcatgaagc ccccaacgtc aagctcaaga ccgtggtcgg acttgtcgac acgatgaagc
    15181 cgctgctgca atcaggcgaa ctggacctga tggtgggaac ggaaagcgcc aaggatgcgc
    15241 agtttgcatc ggaactgctt gctgaggacc agatcgtcgt cgcggcctac aagaagcacg
    15301 aactgttctc ccgagcggtc aaactggaag atctgacgcg ctaccgctgg gtactccagc
    15361 ccccgggagc gcccacccgc gactggctgg accacacctt cgagcgcaaa ggatttcccc
    15421 ggcctcaggt gcaggtggaa gcaagcatgc tgttcattct gcctgcactg attgcggaaa
    15481 caggcttgct gagctttgtc tcgcgccacc acctggggca aaccaagggc acgaccctca
    15541 gagagatacg cctgccggcc acgaccatgc gccgtcgcct ggtcgtcacc taccgcaagg
    15601 acggctatct atcgccggcc gcctcgcgca tgatcgccat cctcgccgca acggcgaagc
    15661 agaatctgag cccggaccga tagtcgagcc ggttttcacc cggcgtccga aaccgattga
    15721 ggcttgcgga tactcgccct gacagggttc ggtgaacgcc cgccccccgg ggcgccaagt
    15781 ggcgcctttg cgtccccgtc taatgatcgc ggcccagagt gggaacccgc tgctggggcc
    15841 aataacccgt ccgtcgcttc ttcaccagca cttcttgcgc ctccctcgcg cacttgaaga
    15901 ggggggccat cggattggaa ggaggctgaa tccgggaatg ccccaatacc aaaagcgaaa
    15961 tttatggttc cgaaaaaata atgaaatgga ttcgcaggat tccggcacac ataatcaact
    16021 gcccctcgtc cctttttgca ggagcagcgc acttcggtac gcgatgcaac aagggatcgg
    16081 atgaaggcgc ctggaacggc gcacaccctt tgataacaac ggagacgtta atgaacacca
    16141 tcggtcgcgt ttttctcgcc gccgccctgt ctgccacgat gacgagcgcc gcgtttgcac
    16201 aggctgagta tccgaacaaa cccattcgcc tgatcgtccc gttcccgccg ggaggaggaa
    16261 ctgacatcgt cgcgcgtgtg gtggccaaca agcttcagga agagctcaag tggacgatcg
    16321 tcgtggaaaa ccgcccgggt gcgggcggaa cgatcggcct ggacgtggca gcgaaggccc
    16381 ctgcggacgg atataccatc gtcatgggac agaccagcaa cctgacggtg agcccggccc
    16441 tgtatccgcg gctgacgtat aacccggtca gggattttgc cccggtgacg accgttgcga
    16501 cggcaccgct cgtcctggtg acgcggacag actctccgtt caagaccgta gccgacgtca
    16561 tcagcgcagc gaaggcaaaa ccgggtgaaa tcaactttgc ctcgcccgga aacggcaccg
    16621 tcgcgcatct gaccggcgaa ctcttccaga aagcggcaaa cgtgaagctc gagcacattc
    16681 cctacaaggg aacggcacag gcgatgaccg atgtacttgg cgggaaggtc gagttgtaca
    16741 tgtcgtcggt cccctcggcg atgtcgcaga tcaagagcgg caagatccgc ccgatcgtgg
    16801 tcacgtcggc caagcgttcg agcgatctgc ccaacgttcc gaccatgcat gaatccggat
    16861 acaaggactt cgatgcgttc acctggttcg gtctgctggt gccggcaggt acgccggcgc
    16921 ccgtcatctc gcgcctgaat accgaggtca acaaggttct tgcacgcaag gatgtgcagg
    16981 aacgaatcag cgccgaaggc ggcaacgtcc agggcagcac accggaagcg ttcgctgccc
    17041 tgatcaagga agaaatcccg aggtgggcag tggtcgtcaa ggaatccggc gccacgatcg
    17101 actgacacat tgcgtctcct ccgcacggaa gctgaagccc gacagggcag cagcttccgt
    17161 tttttcatat agcaccgcgc cgcattcgcc ttccaccgac gacatggccc ataccggcca
    17221 gagggaacct ggacatccca gcatcggagc atgaaatcca tgacacatcg caactacaag
    17281 gacattctgg gaggcctgat cctggtcgtc ctcggattgt tcgtcgcgta ctactcgacg
    17341 cagcattatc agatgggaac cctgacccga atgggtcccg gagcgtttcc gcgtgcactg
    17401 ggctatctgc tcgcgggact cggtgtcctg atactggtac ccgcattctt ccgcagcggc
    17461 acccgcatga tttttgactg gcgccccttc cttgcatgcg ccaccagcat tctggtcttc
    17521 gccatcacgg tgaagtatgt cggcatgatc cccgccatcg tgctgctcgt cgtcacggcc
    17581 gctcttgcgg atgacaagct gagcccgaaa ggaatcattg cattggccat cgccgcttcc
    17641 atactcggcg tcgtgatatt cagctacggc ttcgaaatgt cgattccgct tgtgaagtgg
    17701 ggcgggtgaa gaaatggatc tcgcaaacct tgctggcaac atggccctcg ggctctcggt
    17761 tgcattctcg tacaccactc tgctctactg cttcatcggt gtcctgctcg gcactttcgt
    17821 cggcgttttg ccgggtatcg gttcgctggc ggcgatcgcg ctgctcttgc caatcacctt
    17881 ccatctggat ccaacctcgg ccgtcgtgat gctggccggt gtctactacg gctctacgta
    17941 cggcggatcg acggcgtcca tcctgctcaa cctgcccggc acgccatcct ctgccgtggt
    18001 gtgcctcgac ggctatccaa tggcccagca aggccgggcc ggggtcgcgc tcttcatgac
    18061 gaccatcgca tcattcgtcg gcggcagtct cggcatcatt atcctgatgc tgttcgcacc
    18121 ggcgattggc gaatttgcgt tgcagttcgg gccagccgag tacttcgcca tgatgctgct
    18181 gggtctggtc gccgcctcga ccatatcggc aggctctccg atcaagggaa tcgcgatggt
    18241 ggtactcggc gtcctgatcg ggatcatcgg caccgacgtc aacagcggca cacaacgctt
    18301 gaccttcgat acgccggaac tggccgacgg gatccacctc gttgccatcg cgatgggcat
    18361 ctttggtgta gcggaagtca ttgccagcat ccgcaacgtc agacaccgca aggtggacaa
    18421 caagagcatc acgttcaggt ccatggtgcc gacgcgagac gattggcgcc gctcatgggg
    18481 cccgatggcg cgaggcacgg ccataggatc gttcttcggc gcactgcccg gcacgggcgg
    18541 caccatcgcc tcgttcatgt cctatgcggt ggaaaagaag gtgtcgaagg accccgagcg
    18601 cttcggcaag ggggcgatcg aaggcatcat gggcccggaa actgcaaaca atgcctgcga
    18661 ccagacggcc ttcattccga cactcacgct cggcatcccg ggcagcgtgg tgatggccct
    18721 gatcatcggc gccctgatga tccacggtat tgctcccggg ccgtccttga tggtcgagaa
    18781 cccggatctg ttctggggcc tgatcatgag cttctggatc gggaacatca tgctccttgt
    18841 gattaacatt cccctcattg gtgtctgggt tcgcgtactg tccgtgccgc atcacgtgct
    18901 gtatcccatc atcctgatgt tcatctgcat gggagcgtac agcgtcaaca acagcaactt
    18961 cgacgtctac ctggtactgg ccttcggcgt aatcggctac ctgatgcggg tactggaatt
    19021 tcctccggcg ccattactgc ttggtctggt gcttggcccc atgatggagg agtatctgcg
    19081 tcgtgcgctg ttgctgtcgc gcggcaattt ctccaccttc ctggagagtc cgatcagcgc
    19141 gacgctgatc tgcctcactg gagtattgct cctttggtca ctagtggctc tcgttatgcc
    19201 gcgcaggccc aaggtcgtac gcagtgttgc ccctccagca gcggaattga gcaccgagtg
    19261 attcggagtg caaccagcgt cttgacatcc ccgatgccgg tcttcgctgc ggagtcacta
    19321 ttccgcgtaa tggcgccact ggtgctcgac gcgatggcgc aacttcgagt ggacgatttc
    19381 gggactcgat cggggggaga aggatgcgag tttctcgcgt ctttgcaacc ccgattccgg
    19441 gcggcttagc gagtattttt tgtcacctgg cgacatgggc cggctctccg aggacctcgg
    19501 tttctcggat caggcgcatt tcacccgttt catccgcggg caccctgggc gttacgccga
    19561 gcgagtactg gcgggtggtg cagggtatgg ggatgcggcc tgaacagcat cgccggggtg
    19621 gaaggcccat ttgcgccgcc tggtccacac aggccggtcc gcctctggcg atcggcccct
    19681 tccggagccg gatgtcgtgg cgcgaccgat acgcggaagc gctggaaaac agcaggtcgc
    19741 ggttggcacg cgcctgcctg gtcaggcaag actgccgtgt tcctgtcgcg cgaggcgaaa
    19801 cacgctgac
//
LOCUS       DATLGF010000143         7996 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_192774, whole genome shotgun sequence.
ACCESSION   DATLGF010000143 DATLGF010000000
VERSION     DATLGF010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 7996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7996
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192774"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1265)
                     /locus_tag="VM406_04315"
     CDS             complement(132..1265)
                     /locus_tag="VM406_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75217.1"
                     /translation="MRLFPLLLALSFLLLPSVAAAQPERPRQTSALGNAYFAGGDLRI
                     TSPVAGDLIAAGGRLSIEAPVGADAALAGGTVDVRAPIREDLRAAGGSVTISQSIGAD
                     LVAAGGSVQVDRTARIGGSAWLAGSDVRLAGAVGENVQIYANRIVVDAEIAGDVRLAG
                     QEIELTPAARIGGNLNYASPQPPAGLRPEQVGGAVTRQQTPEQWREGRRTPGTGWLHP
                     VFWAAMLAFGAVLTLLFPAAANGVARTLEAHPWRSLLLGLALVFTVPPVAVMTMVTII
                     GLPVGLSLLLLYPIALLLGYLATAFFLSRRAAIALRQGEAPGRGRRILFLALALLVLS
                     LLLAIPFLGPLVAIVATVAGAGALLGWAYLRIRWGPDATARPL"
     gene            complement(1412..2059)
                     /locus_tag="VM406_04320"
     CDS             complement(1412..2059)
                     /locus_tag="VM406_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HVL75218.1"
                     /translation="MSIKPGSRDALLVVDVQNDFLAGGSLPVPGGDAIIPILNRYIAL
                     FKRNRLPVFATRAWHPQRHASFAEQGGPWPPHCVAGTPGAAFPASLALPDDVIVVSKV
                     ANEQRDAYSGFQETDLDWRLRIAGVDRLFVGGMATDYCVLNTVRDALAHGFDVMLLLD
                     AIRAINTRARDGDAAIAEMLARGALGVQLDDLEAGWERRPHPGNARARSSQGRPA"
     gene            2469..2918
                     /locus_tag="VM406_04325"
     CDS             2469..2918
                     /locus_tag="VM406_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVL75219.1"
                     /translation="MAIKDFCNTDVVICDADTAVPDVAGLMRAHHVGDVIVIEERDGR
                     RFPAGIVTDRDIVLEAVSVGLDVKVFTAGDLMTSPLITVNATSGVFETLQTMSRHRVR
                     RIGVVHEDGSLYGVVSADDLIKLLAQELTSLTVAIADQPAKESRLRK"
     gene            2926..4710
                     /locus_tag="VM406_04330"
     CDS             2926..4710
                     /locus_tag="VM406_04330"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVL75220.1"
                     /translation="MDGARADAGQSQRLAAQDRLVRRLAGALSRSGPVQCHETHISWV
                     LVAGGCAYKFKKAVHFDFVDFSTLAARRFYCEEELRLNRRLAPELYLAVVPVTGSVDA
                     PELQDEGGPDSSSDSSLTDTSSHGGHEPGFDGNAAAGPGAGFQRGGHLPARDWQSEGN
                     LPSERPEADEAIEYAVKMRAFEQDALWSTRLHTGRLGREEIDQLAVLVADFHAHAPAA
                     PPETGWGSARVQRAIADENIATIARLLRGDAHTHVLARLKEWDAAETPRMASLIDARK
                     AAGFVRECHGDLHAGNIVTTSGKVQVFDCIEFNERMRWIDVINDIAFACMDLSARGRS
                     DYSTRLLNAWLEESGDYAGLALLRYYLTQRALVRCKVASLRVRQHEDAATTDAAWREA
                     ADYLALAARCTDVPAPFVGVMHGFSGSGKSYLSLLLAEASGAIRLRSDVERKRLHGLA
                     ATTRGGAKLYARSASEAVYALLARRTRQIVEAGYPVIVDAAFLKRAQRDILRQAAMRL
                     GVPFLIVDVQTSQSTMEARVAARSRAGSDASDADLAVLRRQLASDEPLAADERDLAVR
                     VTCESGGEQEALARVLARLPRGYADAAR"
     gene            complement(4689..5600)
                     /locus_tag="VM406_04335"
     CDS             complement(4689..5600)
                     /locus_tag="VM406_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL75221.1"
                     /translation="MRRRIPSTGALIAFEAAARHESFTKAAEDLALTQSAVCRQIAGL
                     EDYLGVALFRRTRRGVKLTEAGLAYSRQVTARLDAVEHDTLALMAHQGRGVTLELAVV
                     PTFATQWLLPRLAPFLRGHLDITVNMATRTRPFLFGESEFDAAIYFGDAGWPGTEAHY
                     LMHENAVPVCSPRLLATLKKRSIAPHDLARLPLLQQSTRPYAWRQWFEAAGLQVAHDM
                     AGPRLELFSMLAQAAMHDMGVALIPPMMIEAELASGRLVVACNHSCASDKAYYLIVPE
                     QKSESAALLTFRQWLEREAALYRAASA"
     gene            5756..6943
                     /locus_tag="VM406_04340"
     CDS             5756..6943
                     /locus_tag="VM406_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HVL75222.1"
                     /translation="MASNKVVFDWENPLLLDTQLTEDERMVRDSARAYAQDKLMPRIL
                     DAFRNERTDREIFNEMGELGLLGATIPEQYGGAGLNYVSYGLIAREVERVDSGYRSMM
                     SVQSSLVMVPINEFGSEEQKRKYLPKLASGEWVGCFGLTEPNHGSDPGSMVTRAKKVD
                     GGYLLSGTKMWITNSPIADVFVVWAKTEDDVIRGFILEKGWKGLSAPAIHGKVGLRAS
                     ITGEIVMDEVFVPEENLMPGVTGLKGPFTCLNSARFGIAWGALGAAEFCYETARQYVL
                     DRKQFGRPLAANQLIQKKLADMSTDISLGLQGCLRLGRMKEEGIAALELTSMMKRNSC
                     GKALDIARVARDMLGGNGISDEFGVIRHVVNLEVVNTYEGTHDVHALILGRAITGIAA
                     FAS"
     gene            6946..>7996
                     /locus_tag="VM406_04345"
     CDS             6946..>7996
                     /locus_tag="VM406_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HVL75223.1"
                     /translation="MAGALSHIRVLDLSRILAGPWAGQILADLGADVIKVERPGSGDD
                     TRGWGPPFLKDREGNPTDVAAYYLCANRNKRSIAVDITRPEGQAVIRALAAQSDVVLE
                     NFKVGGLKQYGLDYDSLKALNPRLIYCSITGFGQTGPYAARAGYDFLIQGMGGLMSLT
                     GLPDGEPGGGPMKVGVALTDVLTGLYAATAVLAALAHRDQGGKGQHIDLALLDVQVAC
                     LANQALNYLTTGASPKRLGNSHPSIVPYQDFPTADGYMILAIGNDGQFARFCEVAGCP
                     ELAADSRFATNKGRVENRSVLIPLLRQKTVFRTTAEWVAALESVAVPCGPINTLAEVF
                     ADPQVVARGLKVEMSH"
BASE COUNT         1349 a         2577 c         2798 g         1272 t
ORIGIN      
        1 ggatcggcag ccagtcgggg tgcatcatcg gcaagcgcgg gggacgggaa gggcggggcg
       61 cgaagtcatg ggcgcgggtc agtctgcggt gcggcctgtt ccaggaggga atgccgtggg
      121 actttatcac atcagagtgg gcgcgcggtc gcgtcgggcc cccagcgtat gcgcaggtag
      181 gcccagccca gcagcgcgcc ggcgccggca accgtggcca cgatggccac cagcggcccg
      241 aggaagggga tcgccagaag caggctcagc accagcagcg ccagcgcgag aaacagaatc
      301 cgcctgccgc gcccgggcgc ctcgccctgg cgcagcgcga tcgccgcccg cctgctcagg
      361 aagaacgcgg ttgcgagata gccgagcagc agggcgatcg ggtagagcag gagcagcgac
      421 aggccgactg gcagaccgat gatggtgacc attgtcatca cggccacggg cggcaccgtg
      481 aacaccagtg caaggccgag cagcaggctg cgccaggggt gcgcttccag cgtgcgcgcg
      541 acgccgttcg ccgcagccgg gaacagcagg gtgaggacgg cgccgaaagc cagcatcgct
      601 gcccagaaca ccggatgcag ccagccggtg ccgggcgtgc gccggccttc gcgccactgt
      661 tcgggcgtct gctggcgggt gaccgcgcca ccgacctgct cggggcgcag tccggccggc
      721 ggctgcgggc tcgcatagtt cagattgccg ccgatgcggg cggccggcgt cagttcgatt
      781 tcctggccgg ccagccgtac gtccccggca atctcggcat cgaccacgat tcggttggcg
      841 tagatctgta cgttttcgcc caccgctccg gccagccgca cgtcgctgcc ggccagccag
      901 gcgctgccgc ctatgcgcgc ggtccggtcg acctgcacgc tgccgccggc cgccaccagg
      961 tccgcgccta tgctctggct gatcgtgacc gagccgccgg ccgcgcgcag gtcttcccgg
     1021 atcggcgcgc gcacgtccac cgtgccgccg gcaagcgcgg cgtccgcgcc gaccggcgct
     1081 tcgatgctca ggcggccgcc ggcggcgatc aggtcgccgg ccaccgggct ggtgatacgc
     1141 aggtcgccgc cggcgaaata ggcattgccc agtgcgctgg tctgccgagg gcgctcgggc
     1201 tgggcggccg caacggaggg aagcaacaga aacgagagag cgagaagtag cgggaaaagc
     1261 cgcatgccat tcctcccttc ctgagcgagg cgcaacgcgt cggactgcca tgccggcggt
     1321 gatggtgttc actcaagcaa tggcccaaac gatgtcgcct ccgcctgcgc ggggcgacag
     1381 gtttgggcaa tcgctcaaac gagccgcgtc tctacgccgg ccggccctgc gagctgcgcg
     1441 cccgggcgtt tcccggatgc gggcggcgct cccatcccgc ttcgaggtca tcgagctgca
     1501 caccgagcgc gccgcgcgcg agcatttccg cgatcgccgc atcgccgtca cgtgcacgcg
     1561 tgttgatcgc gcgtatcgcg tcgagcaaca gcatcacgtc gaagccatgc gcgagcgcat
     1621 cgcgcaccgt gttcagcacg caatagtcgg tcgccatccc gccaacgaac aggcggtcca
     1681 cgccggcgat gcgcaggcgc cagtcgaggt cggtctcctg gaagcccgaa taggcgtcgc
     1741 gctgctcgtt ggctaccttg gataccacga tcacatcatc cggcagggca agcgaggcgg
     1801 ggaaggctgc gcccggtgtt ccggccacgc agtgcggcgg ccagggcccg ccttgctcgg
     1861 cgaaggaagc gtgccgctgc ggatgccagg cgcgcgtcgc gaacaccgga agccggttgc
     1921 gtttgaacag cgcgatatag cggttgagaa tcggaatgat ggcatcgcct cccggcaccg
     1981 gaaggctgcc acccgcaaga aagtcatttt gaacatcgac cacgagcagg gcgtcgcggc
     2041 tgccgggttt gatcgacatg gcaccaagcc tgataggacg gttcgtaaca ggctctagct
     2101 taggctttgc aacatcggca gagaaatcgg cggggacgcg atttgatgca agtcaaagcc
     2161 gtgaacttca tgcagcgtga agctgggaag ttgccgtggc cgctctgagc gatacgccat
     2221 gtccgcgtcg acgaagaccg cgttcgcagc acgcgggctg gccgattctg gcatcggcat
     2281 gcgcacggac cttgccggcg atgccccata tccggattgc gcgtacagcg tggctgaggc
     2341 tgaatacggt gggcgagccg cgcgttgcag gaaccggcgc gcatggccga cagacaaagc
     2401 ggcaatgcgt gagccggatt ccgcttccgg acggcagctt gtctcccgtc atttctgtgg
     2461 aggtgcgcat ggcaatcaag gatttttgca ataccgatgt ggtgatctgc gacgccgaca
     2521 cggccgtgcc ggatgtcgcc gggttgatgc gagcgcatca cgtcggcgac gtgatcgtga
     2581 tcgaagagcg cgacggcagg cgttttccgg ccgggatcgt caccgaccgc gatatcgttc
     2641 tcgaggcggt gtcggtcgga ctggacgtga aggtgttcac cgccggcgac ttgatgacct
     2701 cgccgctgat caccgtgaat gcgacttccg gcgtgttcga aaccttgcag acgatgagcc
     2761 gtcaccgggt gcggcgcatc ggcgtcgtgc acgaggacgg ttcgctgtac ggcgtggtca
     2821 gcgcggacga cctgatcaag ctgctggccc aggaactgac ctcgctgacc gtcgcgatcg
     2881 ccgatcaacc cgcgaaggaa tcgcggctgc gcaagtaggc cgtcgatgga tggcgcgcgc
     2941 gcagacgccg ggcagtcgca gcgcctggct gcccaggacc ggctggtgcg gcgcctcgcc
     3001 ggagccctta gccgcagcgg gccggtgcag tgccacgaaa cgcacatctc ctgggttctc
     3061 gttgccggcg gctgcgcgta caagttcaag aaggcggtgc atttcgattt cgtcgatttc
     3121 tccacgctgg ccgcgcgccg tttttactgc gaggaggagt tgcggctgaa ccgccggctt
     3181 gcgccggagc tgtatctcgc ggtcgtcccg gttaccggca gcgtcgatgc gcctgaattg
     3241 caggacgagg gcggaccgga cagttcaagc gattcatctc ttaccgatac atcaagccat
     3301 ggcgggcatg agcccggttt cgacgggaac gcggctgccg gccccggagc cgggttccag
     3361 cggggcggcc atctgcctgc acgggattgg cagagcgagg gcaatctccc atccgagcga
     3421 ccggaggcgg acgaggcgat cgaatacgcg gtcaagatgc gcgcgttcga acaggacgcg
     3481 ttgtggagta cgcggctgca tacgggccgc ctcggccgtg aggaaatcga tcagctagcc
     3541 gtgctggtcg cggattttca tgcgcacgca ccggccgcgc cgccggaaac cggctggggc
     3601 agcgcgcggg tgcagcgcgc aatcgccgat gagaacattg cgaccattgc ccgattgttg
     3661 cgcggtgacg cgcacacgca cgtgctcgcc cggctgaagg aatgggacgc cgccgaaacg
     3721 ccgcgcatgg cctcgctgat cgacgcgcgc aaggcagccg gattcgtgcg cgagtgccat
     3781 ggcgacctcc atgcgggaaa catcgtcacg acgtccggca aggtccaggt gttcgactgc
     3841 atcgaattca acgagcgcat gcgctggatc gacgtcatca acgacatcgc gtttgcctgc
     3901 atggacctga gcgcgcgcgg caggagcgat tactcgacgc gcctgctcaa tgcctggctg
     3961 gaggagagcg gcgactacgc cggcctggct ctgctgcgct actacctgac tcagcgtgcg
     4021 ctggtgcgct gcaaggtcgc ctcgctgcgc gtgcggcagc atgaagacgc agcgacgacc
     4081 gacgcggcct ggcgggaagc ggccgattac ctggcgctcg ccgcgcgctg taccgatgtg
     4141 ccagcgccgt tcgtcggcgt catgcatggc ttctccggca gcgggaaatc ctacctgtcg
     4201 ttgctgctgg ccgaggcgag cggcgcaatc cgtctgcgtt cagacgtcga gcgcaagcgg
     4261 ctgcacgggc tggcggccac cacgcgcggc ggcgccaaac tgtatgcgcg cagcgcgagc
     4321 gaggccgtgt acgcgctgct cgcccgacgc acgcggcaga tcgtcgaggc gggctatccg
     4381 gtgatcgtcg acgccgcctt cctcaagcgc gcgcagcgcg atatcctgcg tcaggccgca
     4441 atgcggctgg gcgtgccgtt cctgatcgtg gatgtacaga cttcgcagtc gacgatggag
     4501 gccagggtgg ccgcgcgaag ccgggccggc agcgacgcat cggacgccga tctcgcggtg
     4561 ctgcgccggc agctggcaag cgatgagccg ctcgccgccg acgagcgcga cctggcggtg
     4621 cgcgtcacat gcgaaagcgg tggcgagcag gaagcgctgg cccgcgtgct cgcgcgcctt
     4681 ccgcgaggct atgccgacgc ggcccggtag agcgccgcct cgcgttccag ccattgccgg
     4741 aaagtgagca gcgcggcgct ttccgacttt tgctcgggga cgatcaggta gtacgccttg
     4801 tcgctcgcgc agctgtgatt gcaggcgacg acgagacggc ccgaggccag ctccgcttcg
     4861 atcatcatcg gcggaatcag cgcgacgccc atgtcgtgca tcgcggcctg ggccagcatc
     4921 gaaaacagtt ccaggcgcgg accggccatg tcgtgcgcga cctgcaggcc ggcggcttcg
     4981 aaccattgcc gccacgcgta cggtcgcgtg ctttgctgca gcagcggcag ccgcgcgagg
     5041 tcgtgcgggg cgattgagcg cttcttcagc gtggcgagca ggcgcgggct gcataccggc
     5101 accgcattct cgtgcatcag gtaatgcgcc tcggtgccgg gccaaccggc gtcgccgaag
     5161 tagatggctg cgtcgaattc ggattcgccg aacaggaagg gccgcgtgcg tgtggccatg
     5221 ttgacggtga tgtcgaggtg cccgcgcaag aacggtgcga gcctgggcaa tagccactgc
     5281 gtggcaaaag tgggcaccac ggccagctcc agcgtcacgc cgcgtccctg gtgcgccatc
     5341 agcgccagcg tgtcatgttc gaccgcgtcg agccgggcgg tgacctgccg gctgtaggcg
     5401 agacccgctt cggtcagctt gacgccgcgc cgcgtgcggc gaaacagcgc cacacccagg
     5461 tagtcttcga gtccggcgat ctggcggcag accgcgctct gcgtcagcgc gaggtcctcg
     5521 gccgccttcg tgaagctttc gtgccgcgcc gccgcctcga aggcgatcag cgcgccggta
     5581 ctgggaatcc tgcgccgcat cgtgatggtc cggaatgtgt cggagtgcga ttttcgcaca
     5641 ggtgcgtacc aaatagtcgt ttgcggccgt gccgcggtgc tcctatgatc ggcggctgga
     5701 ggcggcatcc gtgcaggtgc cgacatgagg acacgaagtg acgaagagga acgcaatggc
     5761 atccaacaag gtggtattcg actgggaaaa cccgctgctg ctcgatacgc agctgaccga
     5821 ggacgagcgc atggtgcgcg acagcgcccg cgcctatgcg caggacaagc tgatgccgcg
     5881 catccttgac gcgttccgca atgagcgtac cgaccgcgag atcttcaacg aaatgggcga
     5941 gctggggctc ctcggcgcga ccattcccga gcagtacggc ggcgccggac tcaactatgt
     6001 gtcctacggc ctgatcgcgc gcgaagtgga gcgcgtggat tccggctacc gctcaatgat
     6061 gagcgtgcag tcttcgctgg tcatggtgcc gatcaatgaa ttcggcagcg aggaacagaa
     6121 gaggaagtat ctgcccaagc tggccagcgg cgaatgggtc ggctgcttcg ggctgaccga
     6181 gccgaaccac ggctccgatc ccggctcgat ggtcacgcgc gcgaagaagg tagacggcgg
     6241 ctatctgctg tccggcacca agatgtggat caccaacagc ccgatcgcag acgtgttcgt
     6301 ggtatgggcc aagaccgagg acgacgtgat ccgcggcttc atcctggaga agggctggaa
     6361 ggggctgtcc gctccggcga ttcacggcaa ggttggcctg cgcgcctcga tcaccggcga
     6421 gatcgtgatg gacgaggtgt tcgtgcctga agagaacctg atgcccggcg tgaccggcct
     6481 gaaggggccg ttcacctgcc tgaactcagc gcgcttcggc attgcctggg gcgcgctcgg
     6541 cgcggccgag ttctgctacg agaccgcgcg ccagtatgtg ctggatcgca agcagttcgg
     6601 ccggccgctg gcggccaacc agctgatcca gaagaagctg gccgacatga gcaccgatat
     6661 ctcgctcggc ctgcagggct gcctgcggct gggccggatg aaggaagaag ggatcgcggc
     6721 gctcgaactg acctcgatga tgaagcgcaa ttcctgcggc aaggcgctgg acattgcccg
     6781 cgtcgcgcgc gacatgctgg gcggcaacgg catctccgac gagttcggcg tgatccgcca
     6841 cgtggtgaac ctggaagtgg tcaacaccta cgagggtacg cacgacgtgc acgcgctgat
     6901 ccttggccgc gcgatcaccg gcatcgccgc gttcgcgagc tgatcatggc cggcgcgctg
     6961 tcgcatatcc gcgtgctcga cctgtcccgc atcctcgcgg gaccatgggc cgggcagatc
     7021 ctggccgatc tcggcgccga cgtgatcaag gtcgagcgcc ccggcagcgg cgacgatacg
     7081 cgcggctggg gacccccgtt cctgaaggac agggaaggca acccgaccga cgtcgccgcc
     7141 tactacctgt gcgcgaaccg caacaagcgt tcgatcgcgg tcgacatcac ccgcccggaa
     7201 ggtcaggcgg tgatccgtgc gctggccgcg cagtccgacg tggtgctgga gaatttcaag
     7261 gtcggtggcc tgaagcagta cgggctcgac tacgactcgc tgaaagcgct gaacccgcgt
     7321 ctgatctatt gctcgatcac cggcttcggc cagaccgggc cctatgcggc gcgcgccggt
     7381 tacgacttcc tgatccaggg catgggcggg ctgatgagcc tgaccggcct gccggacggc
     7441 gaaccgggtg gcgggccgat gaaggtgggc gtcgcgctga ccgatgtgct gaccgggctg
     7501 tacgcggcca cggccgtgct tgctgcgctg gcgcatcgcg accagggcgg caagggccag
     7561 cacatcgacc tggccctgct cgacgtgcag gtcgcctgcc tcgcgaacca ggcgctgaac
     7621 tacctgacca ccggtgcctc gcccaaacgg ctcggcaaca gccatccgag catcgttccg
     7681 tatcaggact tcccgaccgc cgacggctac atgattctcg cgattggcaa cgatggccag
     7741 ttcgcgcgct tttgcgaagt ggccggctgc ccggagctgg cggccgattc ccgctttgcc
     7801 acgaacaagg gcagggtgga gaaccgttcg gtgctgattc cgctgctgcg ccagaaaacc
     7861 gtgtttcgca ccaccgccga gtgggtcgcg gcactggagt cggtggcggt tccctgcggt
     7921 ccgatcaaca cgctggccga ggtgtttgcc gatccgcagg tcgtcgcccg cggtctgaag
     7981 gtcgagatgt cgcacg
//
LOCUS       DATLGF010000144         5557 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_132603, whole genome shotgun sequence.
ACCESSION   DATLGF010000144 DATLGF010000000
VERSION     DATLGF010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132603"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VM406_04350"
     CDS             complement(<1..692)
                     /locus_tag="VM406_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL75224.1"
                     /translation="MDKLKQIEAFVCAVEKGSLARAALEQKVTPVMLGRRIDALEKRL
                     GVKLMHRSTRHLSLTEEGTVFLDHCRKLLGDLERAEHMVSEGRHKATGHLVVSAPAAF
                     GRKHVAPHAPAFVAANPGVRISFNLTDRVVDLVREGYDVGIRIGGAIDPNFVAVKLAG
                     NRRVVCASPTYLARHGVPRTLQDLDRHNCLAFNLQGGQQRGWYFQHNGRPVTVKVNGN
                     LDCNDGELLHRWA"
     gene            831..2606
                     /gene="gcl"
                     /locus_tag="VM406_04355"
     CDS             831..2606
                     /gene="gcl"
                     /locus_tag="VM406_04355"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HVL75225.1"
                     /translation="MARMTAAEAAVCVLEIEGVAQAFGVPGAAINPFYAALKRRGSIR
                     HVLARHVEGAAHMAEGYTRARAGNIGLCIGTSGPAGTDMITGLYSAQADSIPILCVTG
                     QAPRARLHKEDFQAVDIEAIARPVTKWAVTVLEPALVPRVFQQAFHLMRSGRPGPVLI
                     DLPFDVQMAEIEFDPQTYAPLPAYKPAATRAQIEKALAMLNEAERPLITAGGGIINAD
                     ASASLVEFAEILGVPVIPTLMGWGAIADDHPLMAGMVGLQTSHRYGNATLLASDFVLG
                     IGNRWANRHTGSIEVFTRGRKFVHVDVEPTQIGRVFEPDYGIVSDAGAALALFVEVAR
                     AWKEAGRLRDRSTWVAQCRERKRTLLRRTHFDSVPIKPQRVYEEMNRAFGRDTCYVST
                     IGLSQIAAAQFLHVYRPRNWINCGQAGPLGWTVPAALGVCAADPDREVVAISGDYDFQ
                     FMIEELAVGAQFRLPYLHVVVNNAYLGLIRQAQRGFDMDYSVQLSFENINAPELEGYG
                     VDHVAVVEGLGCKAIRVKRHEDIQDAFAQARAWMREYRVPVVVEIILERVTNISMGTE
                     IDSINEFEELASGREHAPTAIALLD"
     gene            2619..3398
                     /gene="hyi"
                     /locus_tag="VM406_04360"
     CDS             2619..3398
                     /gene="hyi"
                     /locus_tag="VM406_04360"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HVL75226.1"
                     /translation="MMKLAANLTMLFNEVPFLDRFEQAARAGFRGVEFLFPYGFHADQ
                     IAEKLDAYQLELVLHNLPAGDWEAGERGIACHPGRSAEFREGVEEALHYAKGLGVRQL
                     NCLLGKLPPGTDREEATRTASDNLRYAADMLQEHGIRLLIEPINSFDIPGFSLTHTQQ
                     AVDLIRRTGSSNIYLQYDIYHMQRMEGELANTIKANRDVIGHVQVADNPGRFEPGTGE
                     INYRFLFKLLDEIGYDGWVGCEYKPRAGTVEGLGWRSLHGV"
     gene            3466..4356
                     /locus_tag="VM406_04365"
     CDS             3466..4356
                     /locus_tag="VM406_04365"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HVL75227.1"
                     /translation="MAKVGFIGLGIMGAPMAGHLQDGGHKLYLHNRRNPPFELIEGGA
                     TVCASGAEVAKRADIIITMVPDTPDVEDVLFGRNGIAAGLEPGKIVVDMSSISPLATK
                     GFARRINELGCAYLDAPVSGGEVGAKAAALTIMVGGPESAFAEVRPLFELMGRNVTLV
                     GGNGDGQTTKVANQIIVALTIQAVAEALLFASKAGADPAKVREALMGGFASSRILEVH
                     GERMIRRTFSPGFRIELHQKDLNLALQGAKALGIPLPNTATAQELMNACAAHGMSALD
                     HSALCRAIELMANHEIATVG"
     gene            4353..>5557
                     /locus_tag="VM406_04370"
     CDS             4353..>5557
                     /locus_tag="VM406_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HVL75228.1"
                     /translation="MKRIDPRRLLLDMYASAVAAVTAEHCLPRFLPAPPRGRTIVIGA
                     GKGAAAMALAVERHWQGELAGLVVTRYGHGAPCERIEVVEAAHPVPDAAGRAAAERML
                     QMVQGLGEDDLVLCLISGGGSALLALPAPGITLADKQALNKALLRSGASISEINCVRK
                     HLSAIKGGRLALACAPARVVTLLISDVPGDDPGVIASGPTLPDPTRCADALAILRKYG
                     IAIPAAVERHLASGAGETPKPGDVRFARNSTAVIACAADALEAAAATARAAGITPYIL
                     SDDLEGEAREAARVLAALARRSALHDQPFARPCVLISGGETTVTVKGHGRGGRNAEFL
                     LSLAVSLDGLPGVSAIACDTDGIDGSEDNAGALYLPDSMARAAALGINAKAMLENNDG
                     YGFFDALGDL"
BASE COUNT          953 a         1787 c         1918 g          899 t
ORIGIN      
        1 gcccagcggt gcagcagttc cccgtcgttg caatcgaggt tgccattcac cttgacagtg
       61 accggcctgc cgttgtgctg gaaataccag ccgcgctgtt gcccgccctg caggttgaag
      121 gccaggcagt tgtggcgatc caggtcctgc agcgtcctgg gtacgccatg acgggcgaga
      181 taggtgggcg atgcgcagac gacgcggcgg ttgccggcga gcttgaccgc gacgaagttc
      241 ggatcgatcg cgccgccgat gcgaatgccc acatcgtagc cttcccggac gaggtcgacc
      301 acgcggtccg tcaggttgaa cgagatgcgc accccgggat tggcggcgac aaaggccggt
      361 gcatgcggcg ccacatgctt gcggccgaac gccgcgggcg cggacaccac cagatgcccg
      421 gttgccttgt gccggccctc ggagaccatg tgctcggcgc gttccaggtc gcccagcagc
      481 ttgcggcagt gatcgaggaa gaccgtgcct tcctcggtca gcgacaggtg tcgcgtggaa
      541 cgatgcatca gcttgactcc tagccgcttc tccagcgcgt cgatccggcg gcccagcatc
      601 accggcgtca ccttctgctc cagcgcggcg cgcgccaggc tgcctttttc caccgcgcag
      661 acgaaggctt cgatctgctt gagcttgtcc attgagttcc ccccgcgctg tccctggtcg
      721 attattccat accgggggta tcgaataaaa cgatttctgg tgttcttatc aaaccagtga
      781 tcgaccatta gcatcttctc caacaacaga tcgcggacat aggaggagac atggcaagga
      841 tgacggcggc cgaggccgca gtgtgcgtgc tggaaataga gggggtggcg caggcattcg
      901 gtgtgcccgg ggcagcgatc aatccgttct acgcggcgct gaaaaggcgc ggcagcatcc
      961 gccatgtcct tgcccgtcat gtcgaaggcg ccgcgcatat ggcagagggc tatacccggg
     1021 cgcgggccgg caacatcggc ttatgcatcg gcacctcggg gccggcaggc acggacatga
     1081 ttaccggcct gtattcggcc caggccgatt ccattcccat cctctgcgtg accgggcagg
     1141 cgccgcgcgc tcggcttcac aaggaggact tccaggcggt ggatatcgag gccatcgcca
     1201 ggccggtcac caagtgggcg gtcaccgtgc ttgaaccggc gctggtgccg agggtgttcc
     1261 agcaggcttt ccacctgatg cgctcgggcc gcccagggcc ggtgctgatc gatctgccct
     1321 tcgatgtcca gatggccgaa atcgagttcg atccgcagac ctacgcaccg ctgcccgcct
     1381 acaagccggc ggcgacgcgc gcccagatcg aaaaggcgct ggccatgctc aacgaggccg
     1441 agcgcccgct gatcaccgcg ggaggcggga tcatcaatgc cgacgcatcc gcttcgctgg
     1501 tggaattcgc cgagatactc ggcgtgccgg tgattccgac actgatgggc tggggcgcga
     1561 tcgcggacga ccaccctctg atggccggca tggtcggcct gcagaccagc caccgctacg
     1621 gcaatgccac gctgctggct tcggatttcg tgctcggcat cggcaatcgc tgggccaatc
     1681 gccacaccgg gtcgatcgag gtgttcacgc gcgggcgcaa gttcgtgcac gtggacgtcg
     1741 aaccgaccca gatcggccgc gtgttcgagc cggactacgg gatcgtctcg gacgccggtg
     1801 cggcgctcgc gctgttcgtc gaagtcgcgc gcgcatggaa ggaggccgga cggctccggg
     1861 accgcagcac gtgggtcgcg cagtgccggg agcgcaagcg cacgctgctg cgccgcacac
     1921 acttcgatag cgtcccgatc aagccgcagc gggtgtacga ggagatgaac cgcgcgttcg
     1981 ggcgcgacac ctgctatgtc agcacgatag gactgtccca gatcgccgcc gcgcaattcc
     2041 tgcatgtata caggccgcgc aactggatca actgcggaca ggcagggccg ctgggttgga
     2101 cagtgcccgc cgcgctcggt gtgtgcgcgg ccgatccgga tcgcgaggtg gtggcgatct
     2161 cgggcgacta tgacttccag ttcatgatcg aagagcttgc cgtcggagcc cagttcaggc
     2221 tgccctacct gcacgtggtg gtgaacaacg cctatctcgg gctgatccgc caggcccagc
     2281 gcggcttcga catggattac agcgtgcaac tgtcgttcga gaacatcaat gcgccggaac
     2341 tcgagggcta cggcgtcgac catgtcgcgg tcgtcgaagg actgggctgc aaggcaatcc
     2401 gggtcaagcg gcacgaggac atccaggatg cgttcgccca ggcgcgcgcg tggatgcgcg
     2461 agtaccgcgt gccggtcgtg gtcgagatca tcctggagcg cgtgaccaac atatcgatgg
     2521 gcaccgaaat cgacagcatc aacgagttcg aagaactggc gagcgggcgc gagcacgcgc
     2581 ccactgccat tgccttgctt gactgaggga ggaagacgat gatgaagctc gcagccaacc
     2641 tgaccatgct cttcaacgag gttccgttcc tggaccgctt cgagcaggcg gcgcgcgcgg
     2701 gttttcgcgg cgtggagttc ctgtttccgt atgggttcca tgcggaccag atcgccgaga
     2761 agctcgatgc gtaccagctc gaactggtgc tgcacaatct gccggccggc gactgggaag
     2821 cgggggagcg cggcatcgcc tgccacccgg gcagaagcgc ggagtttcgc gagggcgtcg
     2881 aggaggcgct gcattacgcg aaggggcttg gcgtgcggca gctcaactgc ctgctcggca
     2941 agctgccgcc gggcacggac cgggaggagg cgacgcgcac ggcgtctgac aacctgcgct
     3001 acgcggccga catgttgcag gagcacggga tccgcctgct gatcgagccg atcaacagct
     3061 tcgatatccc cggcttttcg ctcactcaca cccagcaggc ggtggacctg atccggcgca
     3121 ccgggtcgtc caacatctat ctgcaatacg acatctatca catgcagcgc atggagggcg
     3181 aactcgccaa caccatcaag gccaatcgcg acgtgatcgg tcatgtccag gtggcggaca
     3241 accccggacg cttcgagccg ggaaccggag agatcaacta ccgcttcctg ttcaagctgc
     3301 tcgacgagat cggctacgac ggctgggtcg gatgcgaata caagccgcgc gccggcacgg
     3361 tggaagggct cggatggcgc tcgctgcatg gcgtgtgata tgcgaaacgg cggcacggcc
     3421 gcaggcgcgg cgacatgcgc agcacaacca gaaaggagaa caggcatggc aaaggtaggc
     3481 ttcatcgggc tgggcatcat gggcgcaccg atggcagggc atctgcagga tggcggacac
     3541 aagctttacc tgcacaaccg gcgtaatccg ccgtttgaac tgatcgaagg tggcgccacc
     3601 gtctgcgcgt ccggggctga ggtcgccaag cgcgccgaca tcatcatcac gatggttccc
     3661 gatacgccgg atgtggagga tgtgctgttc gggcgcaacg gtatcgccgc aggtctagag
     3721 ccgggcaaga tcgtggtcga catgagctcg atctcgccgc ttgccacgaa gggcttcgcc
     3781 cggcgcatca acgagctcgg ctgcgcctac ctggatgcgc cggtgtccgg cggtgaagtc
     3841 ggtgccaagg cggccgcgct caccatcatg gtgggcgggc cggagtcggc cttcgccgaa
     3901 gtcaggccct tgttcgagct gatgggcagg aacgtcacgc tggtcggcgg caatggcgat
     3961 ggacagacca ccaaggtggc caaccagatc atcgtcgcgc tcaccatcca ggcggtggcc
     4021 gaagcgctgc tgttcgcgtc caaggccggg gcggacccgg cgaaggtgcg cgaggccctg
     4081 atgggcgggt ttgcttcctc gcgcattctc gaggtgcacg gcgagcgcat gatcaggcgc
     4141 acgttttcgc cgggctttcg gatcgagttg caccagaagg atctgaatct cgcgctgcag
     4201 ggcgccaagg cactgggtat cccgctgccg aatacggcca ccgcccagga attgatgaac
     4261 gcctgtgcgg cgcacggcat gagcgctctc gatcattcgg cgctgtgccg cgcgatcgag
     4321 ctgatggcaa atcacgaaat tgccaccgta ggatgaagcg catcgatccc cgccgcctgc
     4381 tgctcgacat gtacgccagc gcggtggccg ccgtgaccgc cgagcactgc ctgccgcggt
     4441 ttttgcccgc gccgccgcgc ggacgcacga tcgtgatcgg cgccggcaag ggcgccgcgg
     4501 cgatggcgct ggcggtggag cggcactggc agggcgagct ggccgggctg gtggtgaccc
     4561 gctacggcca tggcgcgccg tgcgagcgca tcgaggtcgt ggaagcggcg cacccggtgc
     4621 cggacgcagc cgggcgcgcg gccgccgaac ggatgctgca gatggtgcag gggctcggcg
     4681 aagacgacct cgtgctgtgc ctgatctccg gcggcggctc ggccctgctg gcgctgccgg
     4741 cgcccggcat cacgctggca gacaagcagg cgctcaacaa ggcgctgttg cgcagcggcg
     4801 cgtcgatttc cgagatcaac tgcgtgcgca agcacctgtc ggcgatcaag ggcgggcggc
     4861 tggcgctggc ctgcgcaccg gcgcgggtgg tgacgctgct gatctccgac gtgcccggcg
     4921 acgatcccgg cgtgatcgcc agcggcccca cgctgcccga tccgacccgc tgcgccgatg
     4981 cgctggcgat cctgcgcaag tacggcattg cgattccggc ggccgtcgaa cgccatctgg
     5041 ccagcggggc cggcgagacg cccaagcccg gcgatgtgcg ctttgcgcgc aacagcacgg
     5101 ccgtgatcgc gtgcgcggcc gatgcgctgg aagcggcggc ggccaccgcg cgcgcggccg
     5161 ggatcacgcc ctacattctt tcggacgacc tggaaggcga agcgcgcgag gccgcgcgcg
     5221 tgctggccgc gctggcgcgc cgcagcgcgc tgcacgacca gccgtttgcg cggccctgcg
     5281 tgctgatttc cggcggcgag accaccgtga cggtcaaggg ccacgggcgc ggcgggcgca
     5341 acgcggaatt cctgctgagc ctggcggtga gcctggacgg cctgccgggc gtgtcggcca
     5401 tcgcctgcga caccgacggc atcgacggct cggaagacaa cgccggcgcg ctctacctgc
     5461 ccgacagcat ggcgcgcgca gccgcgctgg gcatcaacgc caaggccatg ctggagaaca
     5521 acgacggcta cggttttttc gatgcgctcg gcgacct
//
LOCUS       DATLGF010000145         5222 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_132737, whole genome shotgun sequence.
ACCESSION   DATLGF010000145 DATLGF010000000
VERSION     DATLGF010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132737"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VM406_04375"
     CDS             complement(<1..288)
                     /locus_tag="VM406_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HVL75229.1"
                     /translation="MPRPRALATLALLPFCVVLHAQDLPANGNAVAVYAAGDIANCRR
                     HLASAAPAGATAELVASALADSPYALVLTLGDHTYPLGEMREFKDCYEPTWG"
     gene            complement(301..669)
                     /locus_tag="VM406_04380"
     CDS             complement(301..669)
                     /locus_tag="VM406_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75230.1"
                     /translation="MEPQEQEGRRQGGGHWMNEPDIGSGDKSPAQRDIEREQRALGAE
                     RGTADQDGGDTLQSGDHIARIATRRMDDGMFEAQVFVRLGREPEIAETYIPAGTYSTE
                     QEARSAARERAQRALDEREF"
     gene            803..1201
                     /locus_tag="VM406_04385"
     CDS             803..1201
                     /locus_tag="VM406_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75231.1"
                     /translation="MNQGHTGSGRDMKPGNDGRIPVRSTEDPAGVDDLGLPADARPDG
                     TRAASEVRPDPERTQANDNEPVRTGTVPLEENQQHPSDPLKPGEADEYGTRLEKLIAY
                     IEPPGHEVKDDDIFDPGHMTPGAPPVDNRS"
     gene            1251..1529
                     /locus_tag="VM406_04390"
     CDS             1251..1529
                     /locus_tag="VM406_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75232.1"
                     /translation="MPSKERYDQYAIELNQRFDDLVQWAIANWPREDFPLMQSDFDQA
                     RRQIAEIAGPRLGEPGEQQDGKPVVPDPPGLDDGRKGEFRNLNPMPWP"
     gene            1655..2041
                     /locus_tag="VM406_04395"
     CDS             1655..2041
                     /locus_tag="VM406_04395"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HVL75233.1"
                     /translation="MFKKIKSLFVYALIAAFSFISFTQPARAAMIGTDQVMSAELAQQ
                     NHQKIAAALERADVIAQLEQFGVSKEAAAERVAALTDAEAAMLAQHIDSMPAGGSVLG
                     WIGFIVVVLIITDLLGLTRIFPFTRT"
     gene            2048..3016
                     /locus_tag="VM406_04400"
     CDS             2048..3016
                     /locus_tag="VM406_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA2778 family cysteine peptidase"
                     /protein_id="HVL75234.1"
                     /translation="MQGAILRPRQRWLPGVFLVLVLLLSGCATQTHNLLQGRDASIPP
                     RQELVATPFFPQELYQCGPASLATVLQVAGVSATPEQLVSQVYIPERQGSLQTEMIAA
                     ARRHGTLAVTIPPRLDALLQEVANGTPVIVLQNLSLPVQPVWHYAVVIGYDLDRREVV
                     LRSGTTEREVMPMRTFEHTWARSNHWAMVAMPAGRLPATAAEDEVAPALVALERVAKP
                     EQAHAAYAAALQRWPDNLILLMGAGNAAYRMGKLDQAATALRRATTVHPDSAPAFNNL
                     ANVLIELGDYDSARVAAERAVALGGQWSSAAQATLETVRRRQMSGL"
     gene            3334..5169
                     /locus_tag="VM406_04405"
     CDS             3334..5169
                     /locus_tag="VM406_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75235.1"
                     /translation="MSGPPVVVAGSDLMISEVATNYFSDDIAWFEVYNPTGNPISLAA
                     YELRASHIDITTRASSASPARFALPAVTVDPGAYLVIAGRVFNALKANSQMVYVRSEN
                     LVPFWTAHGSVELVRNGATVDFVRFGNSTAAPLSANAWRSGPVPALPSGPNEHGKSIV
                     RLLSAAMPDTNTAADWALVNFATPAGPNDVNPGVTDSDGDGIPDSAKRPGGTYAGLDL
                     YAMGARAGRRDIFMEVDYMTGTDPALTPRREALQMMVDAFARRNIGLHIDAGPLYGET
                     TDRAHFNLGGGNAVAFAPCIELATNGTGARAGCTSFYDYKSANFDVRRNLVFHYALFA
                     NSQRQDGAAGSSGVAELNGNDLIVTLGGYGFSTSPGPSLNMLVNLQASTLMHELGHNL
                     GLRHGGNEDANYKPNHYSVMNYMYQFAGLSATPNSAHAAERYYLANGMKNKTYCNLVE
                     NSPCGSDFRIDFSDGTSTPLDENSLLESANIGRGAVAGAFADWDNNGVLAIQRYARNL
                     NPQEGGARTVLRDYDEWSNLAIPFARGRSGNNYGMAADQETLFATHLTARSEQAERND
                     KPLRTNPMNHRAQRRIVEDALPYHFQRSLREAQAARAHSWKKKDE"
BASE COUNT          937 a         1745 c         1665 g          875 t
ORIGIN      
        1 gccccaggtc ggttcgtagc aatccttgaa ttcgcgcatc tcgccgagcg ggtaggtgtg
       61 gtcgccgagc gtgagcacca gcgcgtaagg tgaatcggcc agcgccgaag caacgagttc
      121 ggcggtcgca ccggcagggg cggcggacgc cagatggcgg cggcagttgg cgatgtctcc
      181 ggcggcgtag acggccaccg cattgccgtt tgcgggcagg tcttgcgcat gcaggactac
      241 gcagaacggc agcagcgcca gcgtggcgag cgcgcgggga cggggcattg cgcagtggta
      301 tcagaactcc cgttcatcca gggcacgttg cgcgcgctcg cgcgccgccg agcgtgcttc
      361 ctgttcggtg gaataggttc ctgcggggat gtacgtttcg gcaatctctg gctcgcgccc
      421 aaggcgcacg aaaacctgtg cctcgaacat cccgtcgtcc atgcggcgcg tggcgatgcg
      481 ggcgatgtgg tcgccgcttt gcagcgtgtc cccgccgtcc tggtcggccg ttccgcgctc
      541 tgcgccaagc gcgcgctgtt cgcgctcgat gtcgcgctgg gccggcgact tgtccccgct
      601 gccgatgtcc ggttcgttca tccagtggcc gccaccctgg cgccggcctt cctgttcctg
      661 gggttccatg tgacctccgt cttgcatccc tgacttctct gttcagatgg acaaggcgca
      721 cgatagttcg agcgcgccga ttgcgcgcga ttcaccgaac catgcggaat gtccttcatc
      781 taactttttt tcggaggtga gcatgaacca aggacatacc ggctccggcc gcgacatgaa
      841 gcccggcaat gacggacgca ttccagtgcg cagcaccgag gatccggcgg gcgtggacga
      901 tctcggcctg ccggccgatg cccgtcccga cggaacccgc gcggcctcgg aggtgcgccc
      961 cgatcccgag cgcacccagg cgaatgacaa cgaaccggtg cgcaccggca cggtgccgct
     1021 ggaagaaaac cagcagcacc cttccgatcc gctcaagccc ggcgaagccg acgagtacgg
     1081 aacgcgcctg gaaaagctga tcgcatacat cgagccgccc ggacacgagg tcaaggacga
     1141 cgatatcttc gacccgggcc acatgacccc gggcgcgccg ccggtcgaca accggtcctg
     1201 agaccagtcc aggccactga cgccgcgcgc gccgcggcaa ggagagcaat gtgccgagca
     1261 aggagcgata cgatcagtat gcgatcgagt tgaaccagcg ttttgacgat ctcgtgcagt
     1321 gggcgatcgc caactggccg cgggaggact tcccgctgat gcagtcagac tttgaccagg
     1381 cgcggcgcca gatcgccgag atcgccggcc cgcgcctggg cgaaccgggc gaacagcagg
     1441 acggcaaacc cgtcgttccg gacccgccgg gactggacga cggccgcaag ggtgaattcc
     1501 gcaacctcaa cccgatgcca tggccgtgag ggggcggaat ttgcgttttc tcaagaaaac
     1561 tgtatgaggc tgcggaaaat ggaattcaat gcggccactc gctggtcgta gtgaagtggt
     1621 ctgttcattc aacctttcga catacgaggt agccatgttc aaaaagatca agagcctgtt
     1681 cgtctacgcg ctgatcgcag cgttcagctt catcagtttc acgcagcccg cgcgcgccgc
     1741 gatgatcggg accgatcagg tgatgtcagc cgagctggcg cagcaaaacc atcaaaagat
     1801 cgcggcggcg ctggaacgcg cggatgtgat cgcgcaactg gagcagttcg gcgtgagcaa
     1861 ggaagcggcc gccgagcgcg tcgccgcgct gactgacgcg gaagccgcga tgctcgcgca
     1921 gcacatcgac agcatgcctg caggcggcag cgtacttggc tggatcggct tcattgtcgt
     1981 ggtgctgatc attaccgacc tgctcggcct gacccgcatc ttcccgttca cccgcacctg
     2041 agcgcgcatg cagggcgcga ttttacgccc ccggcagcgc tggctgccgg gcgttttcct
     2101 ggtcctcgtc ctcctgctgt caggctgcgc cacgcagacg cacaacctgc tgcaggggcg
     2161 cgacgcttcc atcccgccgc gacaggaact cgtcgccacc ccgttcttcc cgcaggagct
     2221 ctaccagtgc gggccggcgt cgctggccac ggtgctgcag gttgccggcg tgtccgccac
     2281 gcccgagcag ctcgtctccc aggtctatat cccggaacgg cagggcagcc tgcagaccga
     2341 gatgattgcg gcggcgcgcc gccacggcac gctggccgtc accataccgc cgcggctgga
     2401 cgcgctgctg caggaggtgg cgaacggcac gccggtcatc gtcctgcaaa acctcagtct
     2461 gccggtgcag ccggtctggc attacgcggt cgtgatcggc tacgacctcg accggcgcga
     2521 agtcgtgctg cgctccggca ccaccgagcg ggaagtgatg ccgatgcgaa ccttcgagca
     2581 cacctgggcg cgcagcaatc attgggcaat ggttgccatg cctgcgggcc ggctgcccgc
     2641 cacggcagca gaggacgagg tcgcgccggc gctcgtcgcg ctggagcgcg tcgccaagcc
     2701 ggaacaggcc catgctgcct atgcggccgc cttgcagcgc tggccggaca atctcatcct
     2761 gctgatgggt gccggcaatg cagcatatcg aatgggcaaa ctggaccagg ctgcaacggc
     2821 gctgcggcgt gcgaccaccg ttcatccgga cagcgcacca gccttcaaca acctggcgaa
     2881 cgttctgatc gagctcggcg attacgactc cgcccgcgtg gccgccgagc gggccgtcgc
     2941 gctcggcggt cagtggtcga gcgcggccca ggccacgctc gagactgtgc gtcggcgcca
     3001 gatgtcgggg ctgtgagcgc gcaccccggg ctggcaaccg ggttcgttgt caggcagttc
     3061 acagcggccc tcgagcaggg ccgctttcct tttcatcctg ccgttctgag ctgattcctg
     3121 ccgcgcggat gcaacaattt ttgccgcgcg aaaactttcc gcgcggatag atgtctgtag
     3181 gcactcgatt ctccttcggg tgcttacgtg acaagacaaa caagaaaccg ccgtccggcc
     3241 ttgcgcagcc tgggttgcgc gcttgccttc gcggccatgc tctccgcctg cggcggcgga
     3301 tcggagccag gccaggaccc ggcctcggaa ctgatgagcg ggccgcccgt cgtcgtggcc
     3361 ggctcggacc tgatgatttc cgaagtcgcc acgaattact tcagcgacga catcgcctgg
     3421 ttcgaggtct acaacccgac gggcaatccg atatcgctcg ctgcctatga actgcgggcg
     3481 agtcatatcg acatcaccac ccgcgccagc tctgccagtc cggcgcgctt tgcgctgccc
     3541 gcggtcacgg tggacccggg cgcatatctc gtcatagccg ggcgcgtctt caatgcattg
     3601 aaggcgaact cccagatggt ctacgtgcgc agtgagaacc tcgtgccgtt ctggaccgcg
     3661 cacggctcgg tcgagctggt gcgcaatggc gcaaccgtcg atttcgtgcg cttcggcaac
     3721 tccactgccg ccccgctctc ggccaacgcc tggcgcagcg gtccggtgcc cgcgcttcct
     3781 tcggggccga acgagcatgg caagtcgatc gtgcgcctgc tgtcggccgc catgccggac
     3841 acgaacaccg ccgctgactg ggcgctggtg aactttgcga cacctgcggg cccgaacgat
     3901 gtgaatcccg gcgtgaccga ctccgacgga gacggcattc ccgacagcgc caagcgtcca
     3961 ggcggaacct acgccggtct cgacctgtat gcgatgggcg cacgtgccgg cagacgtgac
     4021 atcttcatgg aggttgacta catgacgggc accgacccgg cgctcacgcc gcgcagggag
     4081 gcattgcaga tgatggtcga cgcatttgca cgacgcaaca tcggcctgca catcgacgcc
     4141 ggtccgctgt acggcgaaac gacggatcgc gcccacttca acctcggcgg cggcaatgcc
     4201 gtcgcgttcg ctccatgcat cgagctggcc accaacggga ccggcgcgcg tgccggatgc
     4261 acgtcgttct acgattacaa gagcgcgaat ttcgatgtgc gccgcaatct cgtcttccac
     4321 tacgcgttgt tcgccaattc gcagcggcag gacggcgcgg ccggatcctc gggggtggct
     4381 gaattgaacg gcaacgacct gatcgttacg ctgggcggct acggtttctc gacctcgccg
     4441 gggccgagcc tgaacatgct ggtcaacctg caggcaagca cgctgatgca cgaactcggc
     4501 cacaacctcg gcctgcgcca cggcggcaac gaggacgcga actacaagcc gaatcactac
     4561 agcgtgatga actacatgta ccagttcgcc ggcctgagcg cgacaccaaa ttccgcgcat
     4621 gccgccgagc gctactatct cgccaacggc atgaagaaca agacttactg caacttggtc
     4681 gagaactcgc cgtgcggctc ggatttccgc atcgatttct ccgacgggac cagcacaccg
     4741 ctggacgaaa acagtctgct tgaatccgca aacatcggtc gcggcgcagt ggccggcgcg
     4801 tttgccgact gggacaacaa cggcgtgctc gccatccagc gctacgcccg caacctgaat
     4861 ccgcaggagg gcggggcgcg caccgtgctg cgcgactacg acgaatggag caatctcgcg
     4921 atcccgtttg cccgcggacg cagtggcaac aactacggga tggcagccga tcaggaaacc
     4981 ttgttcgcca cccatttgac ggcgcgctcc gagcaggccg aacgcaacga caagcccctg
     5041 cgcaccaacc cgatgaacca ccgtgcccag cgacggatcg tcgaagatgc gttgccgtat
     5101 cacttccagc gcagtctgcg cgaagcgcaa gcggcgcggg cccattcatg gaagaagaaa
     5161 gatgaatgag cgccgactcg caatgatcag cctcgcgctg tgcgccgccg cgctcgccgt
     5221 ac
//
LOCUS       DATLGF010000146         3958 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_162587, whole genome shotgun sequence.
ACCESSION   DATLGF010000146 DATLGF010000000
VERSION     DATLGF010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162587"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1300
                     /locus_tag="VM406_04410"
     CDS             119..1300
                     /locus_tag="VM406_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HVL75236.1"
                     /translation="MNDPIVIVGAARTPMGAFQGDFSSLAAADLGAVAIRAAVERANL
                     EPNLVNEVLFGNCLMAGQGQAPARQAAIKAGIPVSAGAVTLSKMCGSAMKATMIGYDS
                     ILAGSNEVVVAGGMESMTNAPYLIPKARGGYRIGHGMIYDHMMLDGLEDAYERGRSMG
                     TFGEECAAKYEFTREQQDAYAIESVKRAQAATNDGSFQWEIAPVTVAAKGGEVVIDKD
                     EGPLKAKLDKIASLKPAFKKDGTITAASSSSINDGAAALVLMRESTAKRLGCTPIARI
                     LGHTTHSQEPNWFTTAPVGAIDKLYKKLGIGTKDVDLFEVNEAFAVVPMAAMRDHGIA
                     HDKINLHGGACALGHPIGASGARILVTLIGALRKTGGRLGVASLCIGGGEGTAMAIEM
                     V"
     gene            1417..2085
                     /locus_tag="VM406_04415"
     CDS             1417..2085
                     /locus_tag="VM406_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL75237.1"
                     /translation="MPTALILGASRGIGRELVRQLRAAGWKVYATARDDAALAALNQE
                     GAVAYRVDVTRPDSLAGLGWQLDGEALDLAIYVAGVFGSREGATSPPTAADFDRVMHT
                     NVLGAMQVIPTIAPMVEAARGRFAFISSGMGSIDQTTQSYGWVYRASKAALNMVVKSA
                     AQDYPNAIFLALSPGWVKTDMGGESAPTEAGESVRGMLQVLETATPADSGGFRHFSGR
                     ELPW"
     gene            2114..3244
                     /locus_tag="VM406_04420"
     CDS             2114..3244
                     /locus_tag="VM406_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVL75238.1"
                     /translation="MVLSEQHEMIRDALRAFAQERLAPNAARWDREHVFPKQELKGLA
                     ELGAFGVAVPEQYGGAGLDYTSLALVLEEIAAGDGGTSTIISVNNCPVCSIAMMFANE
                     EQKERFLKPLARGDMLGAFCLTEPHVGSDAAALRTTAVREGNEYVLNGVKQFITSGKT
                     ADVAIVMAVTDKGAGKRGISAFWVPTNTPGYVVARLEEKMGQHSSDTAQILFENCRIP
                     AENLIGEEGQGYKIALSGLEGGRIGIASQAVGMARAAFEAALAYAKERTSFGKTLFEH
                     QAVQFKLADMATQIEAARQLILHAAALKDAGKPCLKEAAMAKLFASEMAERVCSDAIQ
                     VHGGYGYVSDFPVERIYRDVRVCQIYEGASEIQKILIARALN"
     gene            3256..3663
                     /locus_tag="VM406_04425"
     CDS             3256..3663
                     /locus_tag="VM406_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HVL75239.1"
                     /translation="MALKKGYKALVDEAMAQVKTYSVEEARAKLEDANVQFVDIRDVR
                     ELEREGIVPGAYPAPRGMLEFWVDPESPYFKPVFGEDKEFIFFCAAGWRSALATKTAQ
                     DMGMEKVAHIDGGFKAWKEAGAPVGVKESKAKS"
     gene            3814..>3958
                     /locus_tag="VM406_04430"
     CDS             3814..>3958
                     /locus_tag="VM406_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HVL75240.1"
                     /translation="MNKSSNKPCPCGGQNYETCCARYIEGEALAQTAEQLMRSRYSAY
                     TLGK"
BASE COUNT          761 a         1225 c         1378 g          594 t
ORIGIN      
        1 gccaaggagc gcatcctcgc cggcgacatc gccgacgtgt tcccgtatcc gcaggaattg
       61 agattcagct acaaacccct gacccccgcg gcacaggccg catgactgga gacaaaaaat
      121 gaatgacccg attgtgatcg ttggcgccgc ccgcaccccg atgggtgcgt tccagggcga
      181 tttttcctca ctcgccgcgg ccgatctcgg cgcggtggcg atccgcgccg cggtcgaacg
      241 cgcgaacctg gagccgaacc tggtcaatga agtcctgttc ggcaactgcc tgatggccgg
      301 ccagggccag gcaccggccc gccaggccgc catcaaggcc ggcattccgg tatccgccgg
      361 cgcggtgacg ctgtcgaaga tgtgcgggtc ggcgatgaag gcgacgatga tcggctacga
      421 ctcgattctt gccggaagca acgaggtcgt ggtcgccggc ggcatggaat cgatgaccaa
      481 cgccccgtac ctgatcccga aggcgcgtgg cggctaccgc atcggccacg gcatgatcta
      541 tgaccacatg atgctcgacg gcctcgaaga tgcgtacgag cgcggccgct cgatgggcac
      601 cttcggcgag gagtgcgcgg ccaagtacga gttcacgcgc gagcagcagg atgcgtatgc
      661 gatcgagtcg gtcaagcgcg cgcaggcggc gaccaatgac ggttcgttcc agtgggaaat
      721 cgcgccggtg acggtggcgg ccaagggcgg cgaagtggtg attgacaagg acgaaggtcc
      781 gctcaaggcg aagctcgaca agatcgcctc gctgaagccg gcgttcaaga aggacggcac
      841 gatcaccgcc gcgtcgtcgt cctcgatcaa cgacggcgcc gccgcgctgg tgctgatgcg
      901 cgaatcgacc gccaagcgcc tcggctgcac gccgatcgca cgcatcctcg gccacaccac
      961 gcattcgcag gagccgaact ggttcaccac tgccccggtg ggcgcgatcg acaagctgta
     1021 caagaagctc ggcatcggta ccaaggacgt cgacctgttc gaggtgaacg aagccttcgc
     1081 cgtcgtgccg atggccgcga tgcgcgacca cggcatcgcg cacgacaaga tcaacctcca
     1141 cggcggcgcc tgcgcgctcg gccacccgat cggcgcgtcc ggcgcgcgca tcctcgtcac
     1201 gctgatcggc gcgctgagga agaccggcgg caggctcggc gtggcctcgc tgtgcatcgg
     1261 tggcggggaa ggaacggcga tggcgattga gatggtgtga tccaggttcc tcccccttca
     1321 agggggaggt caggaggggg atgggattgc acttgacacc catccctacc ccagccctcc
     1381 ccttgaaggg gagggagcgc agataggaca accaagatgc ccaccgccct catcctcggc
     1441 gcctcgcgcg gcatcggccg cgaactcgtc cgccagctgc gtgcagccgg ctggaaggtc
     1501 tacgccaccg cgcgcgacga cgccgcgctg gcggccctga accaggaggg tgcggtggcg
     1561 tacagggtcg acgtcaccag gcccgattcg ctggccggcc tcggctggca gctcgacggc
     1621 gaggcgctgg acctggcgat ctacgtggcc ggcgtattcg gctcgcgcga gggcgcaacg
     1681 tcgccaccga cggcggccga tttcgaccgc gtgatgcaca ccaatgtgct cggcgcgatg
     1741 caggtgattc cgacgatcgc gccgatggtc gaggccgcgc gcggccggtt tgcattcatc
     1801 agcagcggca tgggcagcat cgatcagacc acacagagct acggctgggt gtaccgggcg
     1861 tcgaaagccg cgctgaacat ggtggtcaag agcgcggcgc aggattaccc gaacgcgatc
     1921 ttcctcgcgc tgtcgccggg ctgggtgaag accgacatgg gcggcgaatc ggcgccgacc
     1981 gaggccgggg aaagcgtgcg cggcatgctg caggtactgg agaccgcaac gcctgcggac
     2041 agtggaggct tccgccattt cagcggacgc gaactgccgt ggtaggcgtt gcacgataac
     2101 gagaaagaga atcatggttt tatccgaaca acatgaaatg atccgcgatg cgctgcgcgc
     2161 gttcgcacag gaacgccttg cgccgaatgc cgcccgctgg gaccgcgaac acgtcttccc
     2221 gaagcaagag ctgaagggcc tggccgaact cggcgcgttc ggcgtggcgg tgccggagca
     2281 gtacggcggc gccggcctcg attacacctc gctggccctg gtgctggaag agatcgcggc
     2341 cggcgacggc ggcacctcga ccatcatctc ggtcaacaat tgcccggtgt gcagcatcgc
     2401 gatgatgttc gccaatgaag agcagaagga gcgcttcctg aagccgctcg cgcgcggcga
     2461 catgctgggc gcgttctgtc tgaccgagcc gcatgtgggc agcgatgccg ccgcgctgcg
     2521 caccaccgcg gtacgcgaag gcaacgaata cgtgctcaac ggcgtcaagc agttcatcac
     2581 cagcggcaag accgccgacg tggcgatcgt gatggcggtc accgacaagg gcgcgggcaa
     2641 gcgcggcatc agcgcgttct gggtgccgac caatacgccc ggttacgtgg tcgcgcggct
     2701 ggaagagaag atgggccagc actcgtccga caccgcgcag atcctgttcg agaattgccg
     2761 cattccggcg gagaacctga tcggcgagga agggcagggc tacaagatcg cgttgtccgg
     2821 cctcgaaggc gggcgcatcg gcatcgcgtc gcaggcggtc ggcatggcgc gcgcggcctt
     2881 cgaggcggcg ctggcctatg cgaaggaacg gacctcgttc ggcaagacat tgttcgagca
     2941 ccaggcggtg cagttcaagc tggccgacat ggccacgcag atcgaggcgg cgcgccagct
     3001 gatcctgcat gcggcggcgc tgaaggatgc cggcaagccc tgcctcaagg aagcggcgat
     3061 ggccaagctg ttcgccagcg aaatggccga gcgggtgtgc tcggacgcga tccaggtcca
     3121 tggcggctac ggctatgtgt ccgactttcc cgtcgagcgt atctaccggg atgtgcgggt
     3181 atgccagatc tacgaaggcg cgagcgagat ccagaagatc ctgattgcga gagcactcaa
     3241 ctgagggagg cgaagatggc attgaagaag ggctacaagg cgctcgtcga cgaggcgatg
     3301 gcgcaggtga agacctacag cgtggaagag gcgcgcgcga agctggagga tgcgaacgtg
     3361 cagttcgtcg acatccgcga cgtgcgcgag ctcgagcgcg agggcatcgt tcccggcgcc
     3421 tatccggcgc cgcgcgggat gctcgagttc tgggtcgatc ccgagtcgcc ctacttcaag
     3481 ccggtattcg gcgaggacaa ggaattcatc ttcttctgcg cagccggctg gcgcagcgcg
     3541 ctggcgacca agaccgcgca ggacatgggg atggagaagg tcgcgcacat cgatggcggg
     3601 ttcaaggcgt ggaaggaggc gggggcgccg gtgggggtga aggagagcaa ggctaagagc
     3661 tagaggaccg gctccttccc cctttcaggg gaaaggttgg gatgggggta gggagtctgc
     3721 gctggcggcg acgctggatc ggagtccgaa cccccacccc aacctttccc ctgaaagggg
     3781 gagggaacag gcaatcgcgt tggacaccag ttgatgaaca aatcgagtaa caagccctgc
     3841 ccttgcggcg gccaaaatta tgagacctgc tgcgcccgct acatcgaagg cgaggcgcta
     3901 gcacaaaccg ccgaacaact gatgcgctcg cgctacagcg cgtacacgct cggcaagg
//
LOCUS       DATLGF010000147         3293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_22506,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000147 DATLGF010000000
VERSION     DATLGF010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22506"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VM406_04435"
     CDS             <1..633
                     /locus_tag="VM406_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVL75241.1"
                     /translation="SGISAFIVERGMPGLSTGAPYRKMGQEGSPVSEVYFNDCRVPAS
                     NLIGGVEGMGFRTAMKVLNKQRIHLAALCIGPAIRMLDEAIRFVTQRSQFGKPVAEFQ
                     LIQAMIADCQTEIHAARALVLETARQRDRGEDVSLTASMCKYFASEMCGRVADRCVQM
                     FGGYGYIADYDIERFYRDVRLFRLYEGTSQIHQLNIAKRTLAAAGYDARG"
     gene            657..1625
                     /locus_tag="VM406_04440"
     CDS             657..1625
                     /locus_tag="VM406_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVL75242.1"
                     /translation="MMKTILAAAALSLAATLPAAADPSYPSKPIRLIVPFAPGGSTDV
                     LGRIIANELRVELGQPVIVENKPGAGGNIGGDLVAKAAPDGYTLLMAAAGPTVINPSL
                     YAKMTFSPTKDLTPVTMLVREHNLMAVHPSVPARTVKEFIAYAKANPNTVNFGSPGNG
                     SPAHLAGELMNQKAGAKMAHIAYKGSGPAVVDLVAGHISMMIDNMPVLLPHVQNGKLR
                     ALAVPSTSRAVAAPEVPTFAEAGMKDFVITAWKGVMAPAGTPPAVINKLQGTMAKIMA
                     KPEMKQRMSEMGAEPVVDTPAEFGRTIQTETAWWAELVKSTGARID"
     gene            1643..2806
                     /locus_tag="VM406_04445"
     CDS             1643..2806
                     /locus_tag="VM406_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVL75243.1"
                     /translation="MSVHNEEAERLREQVREFIETEAIPREHPSLAHDVAALDAVIRE
                     LQEKARERGVYAPQLPKDLGGCGLGWVARAGILEEAGRSFLGPGALNCAPPDQPNMIN
                     LLQNGTPEQKRRYLMPLVEGRVRSCFAMTEPAPGAGSDPGMLAATAVRRGDGWVLNGH
                     KWFISGAVGAAFSIVLARAEEGATMFIVDAGTPGYELVRNIGSMDGYQIGGHGEIRLV
                     DCEVGPDAVLGEVGKGFEYAQARLEPARLSHCMRFIGRASRAIEIAQDYALRRRSFGT
                     LLADLQQVQAMVADSHIDLHASRLMTIDCAARLDAGLSVKHESAMTKIFVSEAVYRVA
                     DRAVQIMGAVGISEDTPVSLILRELRPFRIYDGASELHRATLARRIFKERLRP"
     gene            complement(2784..>3293)
                     /locus_tag="VM406_04450"
     CDS             complement(2784..>3293)
                     /locus_tag="VM406_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVL75244.1"
                     /translation="LYDSLNVREEKLAIVASANHPEAHARRLAISDLAQSPWVVYSAN
                     MPMRLLLEREFHEAGLQFPLNLIETTSAFTTLALLQRNPSMVAVFSIDVARFCTGFGM
                     TTILPVELRSRSDPYFLVTRRDRSLSPVAQLLVQEFLSEPAIAWRDADQVLTGLPLPD
                     ERFKDEVFP"
BASE COUNT          614 a         1051 c         1092 g          536 t
ORIGIN      
        1 tcgggaatct ccgccttcat cgtcgagcgc ggcatgcctg ggctgtcgac cggcgcgcca
       61 tatcgaaaga tgggccagga aggatcgccg gtttcggagg tgtatttcaa cgactgccgt
      121 gtgccggctt caaacctgat cggtggcgtc gaaggcatgg gattccgcac tgcgatgaag
      181 gtgctgaaca agcagcgcat ccatctggcc gcgctgtgca taggcccggc aatccgcatg
      241 ctggacgagg cgatccgctt cgtgacccag cgcagtcagt tcggcaagcc ggtcgccgag
      301 ttccagctga tccaggcgat gatcgccgac tgccagaccg aaatccatgc cgcgcgggcg
      361 ctggtgctgg agacggcgcg gcagcgcgac cgcggcgagg atgtgtcgct tactgcatcg
      421 atgtgcaagt acttcgcttc cgagatgtgc ggccgtgtgg ccgaccgctg tgtgcagatg
      481 ttcggcggct acggctacat cgccgactac gacatcgagc gtttctaccg ggatgtgcgc
      541 ctgttccgcc tgtacgaggg aaccagccag atccatcagc tcaacattgc caagcgcacg
      601 ctggccgcgg ccggctacga cgcgcgaggc tgataaccac ttacaaggag acaagcatga
      661 tgaagactat cctcgcagct gcagcactga gcctcgccgc cacactcccc gccgccgccg
      721 acccgagcta tccatccaag cctatccgcc tgatcgtgcc gttcgcaccc ggcggctcga
      781 ccgacgtgct gggacgcatc atcgccaatg aactgcgcgt cgagctgggg cagccggtca
      841 tcgtggaaaa caagccgggt gcgggcggca atatcggtgg tgatctggtc gccaaggcgg
      901 cgccggacgg ctacacgctg ctgatggccg cggccggacc caccgtcatc aaccccagcc
      961 tgtacgcgaa gatgaccttc agtccgacga aggacctgac cccggtgacg atgctggtgc
     1021 gcgagcacaa cctgatggcg gtgcatcctt ccgtgccggc caggacagtg aaggaattca
     1081 tcgcctatgc aaaagcgaac cccaataccg tcaacttcgg ttctcccggc aacggcagcc
     1141 cggcgcatct ggccggcgaa ctgatgaacc agaaggccgg cgcaaagatg gcgcatattg
     1201 cctacaaggg aagtggcccc gccgtggtcg acctggtcgc cgggcacatc agcatgatga
     1261 tcgacaacat gcctgtcctg ctgccgcatg tgcagaacgg caagctgcgc gcgctcgcgg
     1321 ttcccagcac gagtcgcgcg gtcgccgcgc ccgaggtgcc cacgttcgcc gaggccggga
     1381 tgaaggactt cgtgatcacc gcgtggaagg gcgtgatggc gccggccggc acgccgcctg
     1441 ccgtgatcaa caagctgcag ggcacgatgg cgaaaatcat ggccaagccc gaaatgaaac
     1501 agcgcatgag cgagatgggt gccgagccgg tggtcgacac gccggccgag ttcggtcgca
     1561 cgatccagac cgaaactgcg tggtgggccg aactggtcaa gtcgaccggc gcacgcatcg
     1621 actgagcccg gaacgaatga tcatgtccgt gcacaatgag gaagcagaac ggctgcgcga
     1681 gcaagtccgc gagttcatcg agaccgaggc catcccgcgc gaacatccct cgctcgcgca
     1741 cgacgtcgcc gcgctcgacg cggtgatccg ggaactgcag gaaaaggcgc gcgaacgtgg
     1801 tgtttatgcg ccccagctgc cgaaggacct gggtggctgc ggactcggct gggtggcgcg
     1861 cgccggcatc ctggaggaag ccggacgcag cttcctcggg cccggcgcgc tcaactgcgc
     1921 gccgcccgac cagcccaaca tgatcaacct gctgcagaac ggaacccccg agcagaagcg
     1981 ccgctacctg atgccgcttg tcgagggaag ggtgcgctca tgcttcgcga tgacggagcc
     2041 cgcgcccggc gccggttctg atccgggcat gctcgctgcc accgccgtgc ggcgcggtga
     2101 cggctgggtg ctgaacgggc acaagtggtt catcagcggc gcggtcggcg cggcattttc
     2161 gatcgtgctc gcccgcgccg aagaaggcgc gacgatgttc atcgtcgatg ccggcacgcc
     2221 cggctacgaa ttggtgcgca acatcggcag catggacggc taccagatcg gcggccacgg
     2281 cgagatccgg ctcgtcgatt gcgaagtcgg ccccgatgcg gtgctcggcg aggtcggcaa
     2341 gggattcgag tatgcgcagg cacggctgga gccggcgcgc ctgtctcact gcatgcgctt
     2401 catcggccgc gcctcgcgcg cgatcgagat cgcgcaggac tacgcgttgc ggcgccgttc
     2461 tttcggcacg ctgctcgcgg acttgcagca ggtccaggcg atggtggccg attcccatat
     2521 cgacctgcac gcaagccgct tgatgacaat cgattgcgcc gccaggctcg atgcgggcct
     2581 gtcggtcaag catgaatcgg cgatgacgaa gatcttcgtg tccgaggccg tgtatcgcgt
     2641 cgccgaccgc gcggtgcaga tcatgggcgc agtgggcatc tcggaggaca cgccagtgtc
     2701 gctgatcctg cgcgagttgc gtccgttccg catctatgac ggcgccagcg aactgcatcg
     2761 ggcaacgctg gcgcggcgca ttttcaagga aagacttcgt ccttgaatcg ttcgtccggc
     2821 agcgggaggc cggtcagcac ctgatcggca tccctccagg cgatggcggg ctccgaaagg
     2881 aactcctgca cgagcagctg ggccaccggc gacaggctgc ggtctcgccg cgtgacgagg
     2941 aagtacggat cgctgcgcga acgcagttcc accggaagga tcgtcgtcat gccgaagccg
     3001 gtgcagaagc gcgccacgtc gatcgaaaac accgcaacca tcgacgggtt gcgctgcagt
     3061 agcgcgagtg tcgtgaatgc ggacgtggtc tcgatcaggt tgagcgggaa ctgcaggccc
     3121 gcttcatgga actcgcgttc aagcagcagg cgcatcggca tgttcgccga atacacgacc
     3181 cacggagatt gggcaaggtc cgaaatggcc agccgtcgcg cgtgcgcctc cggatggttc
     3241 gcgcttgcca cgatcgccag cttttcctcg cgcacgttga ggctatcgta aag
//
LOCUS       DATLGF010000148         4748 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_22521,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000148 DATLGF010000000
VERSION     DATLGF010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22521"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VM406_04455"
     CDS             <1..865
                     /locus_tag="VM406_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVL75245.1"
                     /translation="GAWLDEQIQVLQQEMACRRALYAADDGSLVPPLDGATAIIVDDG
                     IATGNTMRAALAAVAEEHPARVLFAAPVGAPDMVQALQAQAEGLCLAAPDDFHAVSPY
                     YTDFTQTTDEEVVRLLAQSRAGLAATQKESRMKKISDLMTRDVATVSPQDSLQRAAQL
                     MKECDVGALPVCDGQTLVGMITDRDITVRASADGQAPDNVKVGEVMSEGITYCFEDQD
                     VEEVLQQMGTEQVRRLPVLTRDKRLCGMVALGDIATRDQTHTDSALEGISQPPAGGQQ
                     GAQGESPSRPS"
     gene            972..1556
                     /locus_tag="VM406_04460"
     CDS             972..1556
                     /locus_tag="VM406_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HVL75246.1"
                     /translation="MKSLKSTLSPSITNMIRMDHTAVLEIFHQYELDASPNTKKGLVD
                     SACLALEIHAQLEEEIFYPAMRAVGSDHAELEKVVPEHNEMRRLIVQLRSMEPTSPGY
                     DETFMQLMRDVMHHIADEETILLADAERKLGDRLGELGADMTTRRLQLSAPRAGEMAT
                     NAVRAFPTSSMLMAAGAVIAGTYLAKRAGSRHHH"
     gene            1627..2499
                     /locus_tag="VM406_04465"
     CDS             1627..2499
                     /locus_tag="VM406_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HVL75247.1"
                     /translation="MEIPGLRGLGPFQLIKESIADFLDDDMLTHASALAFQALFSIFP
                     FIIFLIALLGFLQLTSFFEWLQSHAVMMLPAEAAKPVTEVIAGLQQQQGGLLSFGVIF
                     ALWTASAAVRVVMNATNAAYDVREARPAWKLYPLSIIYTIGIAAMLILSAVLMVLGPQ
                     AMEWIGNQVGFGDTFVTVWTWLRWPAAFLLLILATAIIYYVCPNVEQEFRFITPGAMI
                     AVVVWILASVGFNFYVANFADYNAMYGSIGAIIVLLFYLFISSAVLLFGAEVNAVIEH
                     HAPSGKDKGQKRAD"
     gene            2528..3361
                     /locus_tag="VM406_04470"
     CDS             2528..3361
                     /locus_tag="VM406_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVL75248.1"
                     /translation="MRTVSLPDGTLVPALGQGTWCMAENPRLRSAERDALACGIELGM
                     TLIDTAEMYGDGAAEELVGEVIGGRREQVFLVSKVLPENGSRQGTIAACERSLRRLGT
                     DRIDLYLLHWRGSVPLEETVSAFMRLKRDGKIRYWGVSNFDVSDMEELVRLDSGAMVA
                     TNQVLYNLRRRGIEYDLLPWSVRRQMPTMAYSPIEQGRLVDAEVLKEIADKHMVTPAQ
                     VALSWVLRREDVIAIPKAARIEHVRQNRAALDLVLNEEDLARLDEAYPPPKGPVPLEM
                     I"
     gene            complement(3389..4594)
                     /locus_tag="VM406_04475"
     CDS             complement(3389..4594)
                     /locus_tag="VM406_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL75249.1"
                     /translation="MNSATNAPSHSSIPPLAVPLLSLAAFGSGISLRVTDPMLPFLAQ
                     EFGVALGQAAHVITVFSIAYGLSQLIFGPVGDRYGKYLVVGLASMACALTALLCALAP
                     GYGLLLGARLLAGATAAAIIPLAMAWIGDVVPYQERQPVIARFLIGQVLGLSSGVLLG
                     GLAADIGNWRLPFFFISAVFVVVSVAILSLNRRLPAHARITHRAEGHALVRMVREFHE
                     VLRLPWARTILLAVLLEGAFLYGAFAFIASHAHVRFGISLTTAGALVMLFGLGGFVFA
                     IASAALVRALGEVRLAIVGGALVALSWAGIGLAPAWGWVTIACFAAGIGFYMMHTTLQ
                     INATQMAPERRGAAVSAFASCFFLGQSIGVGTAGLIVEWWGTRAVMLLGAAGVLAVAL
                     RFAANLQRR"
BASE COUNT          929 a         1548 c         1533 g          738 t
ORIGIN      
        1 gggcgcgtgg ctggacgagc agatccaggt actgcaacag gagatggcat gccgacgcgc
       61 gctctacgca gccgacgatg gatccctggt gccgccgctg gacggggcga ccgcgatcat
      121 cgtcgacgat ggcatcgcca ccggcaacac catgcgtgcg gcgctggccg cagtggccga
      181 agaacatccg gcgcgggtac tgtttgccgc gccggtcggc gcgcccgaca tggtgcaggc
      241 cttgcaagcg caggccgagg gactgtgtct tgccgccccg gatgattttc atgcggtcag
      301 tccttattac acagacttta cgcagaccac ggacgaggaa gtcgtgcgct tgctcgcgca
      361 atcgcgcgcc ggcctcgccg ccactcaaaa ggagagcagg atgaaaaaga tttcggatct
      421 gatgacacgc gacgttgcaa cggtcagtcc gcaggacagc ctgcagcgcg ccgcgcaact
      481 gatgaaggaa tgcgacgtcg gtgcgctgcc cgtgtgcgac ggacagaccc tggtcggcat
      541 gatcaccgat cgcgacatca cggtgcgagc cagcgccgac ggccaggcgc cggacaacgt
      601 gaaggtcggt gaagtgatga gcgaaggcat cacctattgc ttcgaggatc aggacgtgga
      661 agaggtgctg cagcagatgg gcaccgagca ggtgcgccgc ctgccggtgc tgacccgcga
      721 caagcggctg tgcggcatgg ttgcactcgg cgacatcgcc acgcgcgacc agactcatac
      781 cgacagcgcg ctggaaggca tctcgcaacc gccggccggc ggccagcaag gcgcccaagg
      841 cgagtccccg tcgcggccgt cctgatacgg cgggagctta ccgtcccttc cggccgtcca
      901 agcaccgccg ggtacggggc ttgctctgca cgccccgacc accctgcgca ccatccctgg
      961 aggaagccac aatgaaatca ttgaaaagta cgctgtcccc gagcatcacc aacatgatcc
     1021 gcatggatca cactgccgtg ctggaaatct ttcaccaata tgagctcgat gcgtcgccga
     1081 acaccaagaa gggcctggtc gacagcgcat gcctggccct ggagatccac gcgcagctcg
     1141 aggaagagat tttctacccg gcgatgcgcg cagtcggttc cgaccacgca gagctcgaga
     1201 aggtggtccc ggaacacaac gaaatgcgcc gtctgatcgt ccagctgcgc tcgatggaac
     1261 cgacctcgcc cggctatgac gaaaccttca tgcagctcat gcgcgacgtg atgcaccaca
     1321 tcgccgacga ggagaccatc ctgctggccg acgccgaacg caaactggga gacaggctgg
     1381 gcgaactcgg agcggacatg accacccggc ggctgcagct ttcggccccg cgcgcaggcg
     1441 aaatggcgac caatgcggta cgcgcattcc cgaccagctc gatgctgatg gcggccggcg
     1501 cggtgattgc cggcacctac ctggccaagc gcgccggttc gcgccatcac cactgaaaac
     1561 ttccaatcct tacccggtgc cgccaggcgc cggggctgac caacaacaag aaggaagggg
     1621 attcccatgg agattcccgg cttgcgcggg ctgggtccgt tccagctgat caaggagtcg
     1681 atcgccgatt ttctcgatga cgacatgctc acgcatgcgt ccgccctcgc ctttcaggca
     1741 ctgttttcga tcttcccgtt catcattttc ctgatcgcgc tgctcggctt cctgcaactg
     1801 acgagcttct tcgaatggtt gcagtcccat gcggtgatga tgctgcccgc cgaagcggcc
     1861 aagccggtta ccgaagtgat cgccggcctg cagcagcagc aaggcggatt gctctccttc
     1921 ggcgtgatct tcgcgctgtg gacggcatcc gccgcggtgc gggtcgtgat gaatgcgacg
     1981 aacgccgcct acgacgtacg cgaagcgcgc cccgcctgga aactgtaccc gctgtcgatc
     2041 atctacacga tcggcatcgc cgccatgctg atcctctccg ccgtgctgat ggtgcttggt
     2101 ccgcaggcga tggaatggat aggcaaccag gtcggcttcg gcgacacctt cgtgacggtc
     2161 tggacctggc tgcgctggcc ggcggccttc ctgctgctca tcctcgcaac ggcgatcatt
     2221 tactacgtct gtccgaacgt cgagcaggag ttccgcttca tcacgcccgg cgcgatgatt
     2281 gccgtcgtgg tctggatcct cgcatcggta ggcttcaatt tctatgtggc caatttcgcc
     2341 gactacaacg cgatgtacgg cagcataggc gcgatcatcg tgctgctgtt ctacctgttc
     2401 atttcctccg ccgtcctgct gttcggggcc gaagtcaatg cagtgatcga acaccacgcg
     2461 ccctcgggca aggataaggg gcaaaagcgt gcggactgaa tccgcgcagc gaaaggaaac
     2521 cggcacgatg agaacagtaa gtcttcccga tggaaccctc gtccccgccc tcggccaggg
     2581 gacctggtgc atggcggaga atccgcgcct gcgctcggcc gagcgcgatg cgctggcctg
     2641 cggaatcgaa ctcggcatga cgctgatcga caccgccgaa atgtatggcg atggcgctgc
     2701 ggaagaactg gtcggcgagg tgatcggcgg acggcgcgag caggttttcc tcgtcagcaa
     2761 ggtcctgccg gagaacggct cgcggcaagg gacgatcgca gcctgcgagc gcagcctgcg
     2821 ccgcctcggc accgatcgca tcgatctcta cctgctgcac tggcgtggca gcgtgccgct
     2881 ggaggagacg gtgtcagcct tcatgcgctt gaagcgcgac ggcaagatcc gctactgggg
     2941 cgtgtccaac ttcgatgtct ccgacatgga agagctggtg cgcctggact ccggcgcgat
     3001 ggtggcgacg aaccaggtgc tctacaacct gaggcggcgc ggcatcgagt acgacctgct
     3061 gccctggagc gtgcgccggc agatgccgac catggcctat tccccgatcg aacagggccg
     3121 cctggtcgac gctgaggtac tgaaggaaat cgcggacaag cacatggtca cgcccgcaca
     3181 ggtggcactt tcctgggtgc tgcgccggga ggacgtgatc gcgatcccga aggcggcacg
     3241 tatcgagcat gtacggcaga accgggccgc gctcgacctc gtgctcaacg aggaagacct
     3301 tgcgcgcctg gacgaggcct atccgccgcc gaagggtccg gtcccgctgg agatgatctg
     3361 acgtggcgca ccgcccgcgc cgcgctcact agcggcgctg caggttcgcc gcaaagcgca
     3421 gcgccaccgc cagcacgccg gccgcgccga gcagcatcac tgcgcgcgtg ccccaccatt
     3481 caacaatcag acctgccgtg ccgaccccga tcgactgtcc aaggaagaag caggacgcaa
     3541 atgccgagac ggccgcgccg cgccgctccg gcgccatctg cgtggcgttg atctgcagcg
     3601 tggtgtgcat catgtagaag ccgatgcccg cggcaaagca ggcgatcgtc acccagcccc
     3661 aggccggtgc gagcccgatc cccgcccagg acagggcgac cagcgcgccg ccgacgatgg
     3721 ccaggcgcac ttctccaagc gcgcgcacca gcgcagcgga tgcaatcgcg aagacgaagc
     3781 cgcccagacc aaatagcatc accagcgcgc cggccgtcgt cagcgatatc ccgaaacgca
     3841 catgcgcatg cgaggcgatg aacgcaaagg cgccgtagag gaaggcgcct tcgagcagca
     3901 cggcgagcag gatcgtgcgc gcccacggca ggcgcagcac ctcgtgaaat tcgcgcacca
     3961 tcctgacgag cgcatggcct tcggcgcggt gggtgatgcg cgcatgcgcg ggcagtcgcc
     4021 ggttcaggga caggatggcg acgctgacga cgacgaacac cgcagaaatg aagaaaaacg
     4081 gcaggcgcca gttgccgatg tccgccgcca gcccgcccag cagcacgccg ctggaaagcc
     4141 cgagcacctg cccgatcaga aagcgcgcaa tcaccggctg gcgttcctgg tatggcacca
     4201 cgtcgccgat ccaggccatc gccagcggaa taatggccgc ggccgtggcg ccggcgagca
     4261 ggcgcgcgcc gagcaggaga ccgtaacccg gcgcgagcgc gcacaacagc gcggtcagcg
     4321 cgcaggccat cgaagccagg ccgaccacga gatactttcc atagcggtcg ccgaccgggc
     4381 cgaagatcaa ctgcgacagc ccataggcaa tcgagaagac ggtgatcacg tgcgccgcct
     4441 ggccgagcgc gacgccgaat tcctgcgcga ggaaaggcag catcgggtcg gtcacgcgca
     4501 acgagatgcc gctgccgaag gcggcgagcg acagcagcgg aacggcaaga ggcggaatgg
     4561 aggagtgcga gggggcgttc gtagcggaat tcatcggttg tggcattgct tcaatgcaac
     4621 gattctagcc ttggacggat tttttggccg gcgcattgga aatcctcgtg cacgcgctcc
     4681 ggaaaagatt gacccaggtc aatcacatgc gcgcccctgg cgggctacgc ttggggccgc
     4741 cgcggatt
//
LOCUS       DATLGF010000149         7405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_3249,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000149 DATLGF010000000
VERSION     DATLGF010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 7405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7405
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3249"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /gene="phoR"
                     /locus_tag="VM406_04480"
     CDS             complement(<1..1229)
                     /gene="phoR"
                     /locus_tag="VM406_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HVL75250.1"
                     /translation="MNPRLVFWIPALLRLCFMLLGAGIIWYFFGLTAGLVAALIGVCT
                     FLYVQLNYLYQLSDWLENPSSVRLPDGWGAWTDVFSRLYKLRREDEKHQTELTEWLAR
                     FRQAMSLLPDGVVIMDDVLFLEWCNPTAEQHLGLQLERDKGMRVTNLVRNPDFIDYII
                     LGRYENPLTLSLRDRKLIVQIIPFENRRQILVTHDATEAQRIEMMRRDFIANASHELR
                     TPLTVINGFLEIACSQPDLDNETRLAHLKLMSEQGQRMQNLVDDMLTLTRLESIEYPL
                     RAERVDVRALLEQIHAEAEALSGGRHAISLDCDGPDIKGGVEELRSAFGNLVSNAVRY
                     TPEGGRIDMSWRVRDGVPQFSVRDNGIGIRPEHISRLTERFYRVDKSRSRQTQGTGLG
                     LPGTALVDAVETLGQAR"
     gene            complement(1245..1934)
                     /locus_tag="VM406_04485"
     CDS             complement(1245..1934)
                     /locus_tag="VM406_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL75251.1"
                     /translation="MAGSETTILVVEDEPPIVELITFSLRDSGWATRPVETATEAWEV
                     IQRDTPQMVLLDWMLPDQSGLKLLSRIRADRNVGQLPVIMLTAKSMEEDKIAGLDSGA
                     DDYITKPFSPRELNSRIKALFRRKSPEHATSVMTAGPVRLDPTSNTVEVNAARVDIGQ
                     TEFRLLKFLLANPDRVFSRSQLLDKVWGDHVAIEERTVDVHVLRLRKALKEAESLIKT
                     VRSVGYMLSEK"
     gene            complement(1936..2643)
                     /gene="phoU"
                     /locus_tag="VM406_04490"
     CDS             complement(1936..2643)
                     /gene="phoU"
                     /locus_tag="VM406_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HVL75252.1"
                     /translation="MPGDHSSKQYDQDLEAIRSKVLLMGGLVESQFKDAVECVLTGDV
                     ARAEEVIKSDENVNRLEVTLDDTCSHLIVKRQPAANDLRTVMATLKVITDLERIGDEA
                     TKIARTAKSLKERGALGSLNPYESIRVMAASAADSLHDALDAFARLDHKQASRIITGD
                     EAIDHEFRSVLRNMITFMMEDPRTISAALDTLWAAKAIERIGDHAKNIAEYVIYVVEG
                     KDIRHTDYVYTHGEQAK"
     gene            complement(2678..3439)
                     /gene="pstB"
                     /locus_tag="VM406_04495"
     CDS             complement(2678..3439)
                     /gene="pstB"
                     /locus_tag="VM406_04495"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HVL75253.1"
                     /translation="MTGKATLEINNLNFFYGNFQGLKNVSLNIYEKKVTAFIGPSGCG
                     KSTLLRTLNRMYDLYPGQRAEGKIVYNGRNILEPGQDVNVLRAKVGMVFQKPTPFPMS
                     VYDNIAFGVRLYENLSRGEMDERVEWALKKAALWGEVKDKLQKSGLSLSGGQQQRLCI
                     ARSVAVKPDVLLLDEPTSALDPISTAKIEELIAELKEDYTITIVTHNMQQAARCSDYT
                     AYMYLGELVEFGETDQIFMNPGKKETQDYITGRFG"
     gene            complement(3452..4300)
                     /gene="pstA"
                     /locus_tag="VM406_04500"
     CDS             complement(3452..4300)
                     /gene="pstA"
                     /locus_tag="VM406_04500"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HVL75254.1"
                     /translation="MMNPVYRRRLWVHRIGIALSVFAMAIGLFFLAWILFTLLVRGFG
                     ALSFALFTETTPAPGSEGGGLANAIVGSLLMVGFATLISTPVGILAGVYLAEYGEESW
                     LAQITRFVTDIMLSAPSIVIGLFVYAIYVANVQHFSGWAGTFAISLIAVPVVVRTTDN
                     MLKLVPNSLREAAFALGAPRWKVATMVRLRAVKAGVVTGVLLAIARISGETAPLLFTA
                     LNNQFFSTDMNAPMANLPVVIFQFAMSPYDNWRSLAWGGALLITFSVLLLNILSRTLF
                     RQKVSS"
     gene            complement(4297..5259)
                     /gene="pstC"
                     /locus_tag="VM406_04505"
     CDS             complement(4297..5259)
                     /gene="pstC"
                     /locus_tag="VM406_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HVL75255.1"
                     /translation="MAARSLAFTVRKQRVQDFLFHKITLIFALSVLLVLLGIIASLMI
                     GAWPAFKEFGPAFVTTVEWDPVNDQYGALIAIVGTLATSIIALLVAFPISFGIAVFLT
                     EICPTALKRPLGTAVELLAGVPSIIYGMWGLFIFAPLFGDYVQPALSATLGQVPLIGP
                     LFTGPMMGIGILTAGLILAVMIIPFIASVMRDVFEIVPAVLKESAYGLGCTKWEVVRK
                     VVLPYTRTGVVGGVMLGLGRALGETMAVTFVIGNAHFLSWSLFSPGNSIASTLANEFA
                     EAESELHLASLFALGLILFVITFIVLAAAKFMLVGMSRREGTKS"
     gene            complement(5443..6486)
                     /gene="pstS"
                     /locus_tag="VM406_04510"
     CDS             complement(5443..6486)
                     /gene="pstS"
                     /locus_tag="VM406_04510"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HVL75256.1"
                     /translation="MQLSKFLKSAVLGFAALAISTSASAQITGAGATFPYPIYAKWAE
                     AYRNATGVSLNYQSIGSGGGIKQIKARTVDFGASDMPLPAKELADSGLVQFPAIIGGV
                     VPVMNVEGVKPGQLRLTGPVLAAIYTGTIKRWDAPEIAALNQGLKLPAQPINVVYRSD
                     GSGTTFLFTDYLSKVSPEFKEKVGSGTAVKWPTGMGGKGNEGVAANVQRIRNSIGYVE
                     VAYAKRNNITHAQMQNRAGEFVQPEDASFQAAAAGADWAKTPGFAIVLTDQPGKGAWP
                     ITGASFILMHKVQQNPARGQEVLKFFDWSFKNGGAMATELDYVPMPASVVKLVQDAWR
                     AELRDPQGKPILQ"
     gene            complement(6632..7282)
                     /gene="upp"
                     /locus_tag="VM406_04515"
     CDS             complement(6632..7282)
                     /gene="upp"
                     /locus_tag="VM406_04515"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HVL75257.1"
                     /translation="MKQDPRFPNLFILDHPLIQHKLTHMRDRETSTRTFRELLRELTL
                     LMGYEVTRELPLTTRRIETPMQAMDAPVIAGRKLAVVPVLRAGIGMSDGLLELIPSAR
                     VGHIGVYRDHETHEPVEYMVRLPDLHERTFILCDPMVATGNSAVHAVDVLRKRGVAPE
                     RILFLALVAAPEGVEVFQAAHPDVRLYVASLDDALDQHAYILPGLGDAGDRIFGTK"
BASE COUNT         1411 a         2287 c         2318 g         1389 t
ORIGIN      
        1 tctcgcgcct gaccgagcgt ttctaccgcg tcgacaagag ccgttcccgg cagacccagg
       61 cccgtgccct gggtctgccg ggaacggctc ttgtcgacgc ggtagaaacg ctcggtcagg
      121 cgcgagatgt gctcggggcg gatgccgatg ccgttgtcgc gcaccgaaaa ctgcggcacg
      181 ccgtcgcgca cgcgccagct catatcgatg cgtccgccct cgggcgtgta gcgcacggcg
      241 ttggacacca ggttgccgaa ggccgagcgc aactcctcaa ccccgccctt gatgtcaggg
      301 ccatcgcagt ccaggctgat cgcatggcga ccgcccgaca acgcctcggc ctcggcatga
      361 atctgctcca gcagtgcacg cacgtcgacg cgctcggcgc gcagcggata ttcgatcgat
      421 tccagccgcg tgagcgtgag catgtcgtcg accaggtttt gcatgcgctg gccctgctcg
      481 ctcatcagct tcaggtgcgc caggcgcgtc tcgttgtcca gatccggctg actgcaggcg
      541 atctcgagaa aaccgttgat gacggtgagc ggcgtgcgca gctcgtgcga agcattggcg
      601 atgaaatcgc gccgcatcat ctcgatgcgc tgcgcctcgg tcgcatcgtg cgtgaccagg
      661 atctggcggc ggttttcgaa cgggatgatc tgtacgatca gcttgcggtc gcgcaggcta
      721 agcgtgagcg ggttctcgta gcggccgagg atgatgtagt cgatgaaatc cggattacgc
      781 accaggttcg tcacgcgcat gcccttgtcg cgctcgagct gcagcccgag gtgttgctcg
      841 gcggtcggat tgcaccattc gagaaacagc acgtcgtcca tgatgaccac gccgtcgggc
      901 agcaggctca tcgcctgacg gaagcgcgcg agccactcgg tcagttcggt ctgatgcttc
      961 tcgtcctcgc ggcgcagctt gtacaggcga gagaacacgt cggtccacgc gccccatccg
     1021 tccggcaggc gcacgctgga tggattttcg agccagtcgg agagttgata gagatagttg
     1081 agctgcacat acaggaaggt gcacacgccg atcagggcgg cgaccagtcc cgcggtcagc
     1141 ccgaagaagt accagatgat cccggcaccg agcagcatga aacacaagcg cagcagggcc
     1201 ggaatccaga acacgaggcg aggattcatg cgagaggaag ggtgctactt ttcggacagc
     1261 atgtatccta cactacgcac ggtcttgatc agggattccg cttccttgag cgccttgcgc
     1321 agccgaagaa catggacgtc gacggtgcgc tcttcgatcg ccacatgatc gccccagacc
     1381 ttgtcgagca gttggctgcg cgagaacacc cggtccggat tcgcaagcag gaatttcagc
     1441 agccggaact cggtctggcc gatgtcgacg cgcgcggcgt tgacttccac ggtgttgctg
     1501 gtcgggtcaa gcctgaccgg cccggccgtc atgacggaag tcgcgtgctc ggggctcttg
     1561 cgccggaaca gcgccttgat gcgcgaattg agctcgcgcg gggagaatgg ctttgtgatg
     1621 tagtcatccg cgccactgtc gagtcccgcg atcttgtctt cttccatgct tttcgcggtc
     1681 agcatgatga ccggcagctg gccgacgttg cgatcggcgc ggatgcgcga aagcagcttg
     1741 agcccgctct ggtctggaag catccagtcc agcagcacca tctgcggcgt gtcgcgctgg
     1801 atgacctccc atgcctcggt tgcggtctcg accggacgcg tcgcccatcc tgaatcgcgc
     1861 aaggaaaacg tgatcagctc gacaatcggc ggttcgtctt cgacgaccag gatggtggtt
     1921 tcgctccccg ccatgttact tcgcctgctc cccgtgcgtg tacacgtagt ccgtatggcg
     1981 gatgtccttg ccctcgacca cgtagatcac gtattcggcg atgttcttcg catggtcgcc
     2041 gatgcgctcg atggccttgg ccgcccacag cgtgtcgagc gcggccgaga tcgtgcgcgg
     2101 atcttccatc atgaaggtga tcatgttgcg cagcaccgag cggaactcgt ggtcgatcgc
     2161 ctcgtcgccg gtaatgatgc ggctggcctg cttgtggtcg agacgggcga aggcgtcgag
     2221 cgcgtcgtgc agcgaatcgg cggccgacgc cgccatgacg cggatcgact catacggatt
     2281 gagcgaaccg agcgcgccgc gctccttcag gctcttggcc gtgcgtgcga tcttggtcgc
     2341 ctcgtcgccg atgcgctcaa ggtccgtgat gaccttcagc gtcgccatca cggtacgcag
     2401 gtcattggcg gccggttggc gcttgacgat cagatggctg caggtgtcgt cgagcgtgac
     2461 ttcgaggcga ttgacgttct cgtcgctctt gatcacttct tctgcgcgtg cgacatcgcc
     2521 ggtgagcaca cattcgacgg catccttgaa ctggctctcg accagcccgc ccatcagcag
     2581 caccttcgag cggatcgctt cgagatcctg gtcgtattgt ttggaggaat ggtcgcctgg
     2641 catctttatc tcctgatttg gtttgtacgg ccgccgctca accgaagcgg ccggtgatgt
     2701 agtcctgggt ttccttcttg ccggggttca tgaagatctg gtcggtttcg ccgaattcaa
     2761 ccaactctcc caggtacatg tatgcagtgt agtccgagca acgcgctgcc tgctgcatgt
     2821 tgtgcgtgac gattgtgatc gtgtaatcct ctttcagctc cgcaatcagc tcttcgatct
     2881 ttgcggtcga aatcgggtcg agcgcggaag tcggctcatc cagcagcagt acgtccggct
     2941 tgaccgccac gctgcgtgcg atgcacaggc gctgctgctg gccgcccgaa agcgacaggc
     3001 cgctcttctg cagcttgtcc ttgacctcgc cccacagcgc ggccttcttc agcgcccatt
     3061 ccacgcgctc gtccatttca ccgcgcgaca ggttttcata aaggcgcacg ccgaaggcga
     3121 tgttgtcgta caccgacatc gggaacggcg tcggcttctg gaacaccatg ccgaccttag
     3181 cgcgcagcac gttgacgtcc tggccgggtt cgaggatgtt gcggccgttg tagacgatct
     3241 tgccttccgc gcgctggccc ggatacaggt catacatgcg gttgagcgtg cgcagcagcg
     3301 ttgacttgcc gcagccggac gggccgatga acgccgtgac tttcttttcg tagatattga
     3361 gactgacgtt cttcaggccc tggaaattgc cgtagaagaa attcaggttg ttgatttcaa
     3421 gcgtagcctt gccggtcatg ggacaccttt gttaagacga gactttctgg cgaaacagcg
     3481 tgcgcgaaag gatgttcagg agcagcacgc tgaacgtgat cagcaatgcc ccgccccagg
     3541 cgagcgagcg ccagttgtcg taagggctca tcgcaaactg gaagatcacc accggcaggt
     3601 tcgccatcgg cgcgttcatg tcggtgctga agaactggtt gttcagcgcg gtgaagagca
     3661 gcggcgcggt ctctccggag atccgcgcga tggccagcaa cacgccggtg accacgccag
     3721 ccttgaccgc gcgcaggcgc accattgtgg cgaccttcca gcgcggcgcg ccgagtgcga
     3781 atgccgcttc gcgcaggctg ttcggaacga gcttgagcat gttgtcggtc gtgcgcacca
     3841 cgaccgggac cgcaatcagt gaaatcgcga acgtgcccgc ccagccggag aagtgctgga
     3901 cgttggcgac atagatcgca tagacgaaca ggccgattac gatcgacggc gccgacagca
     3961 tgatgtcggt gacgaagcgt gtaatctggg ccagccagct ttcctcgccg tactcggcga
     4021 gatagacgcc ggccaggatg ccgaccggcg tgctgatcag cgtcgcaaac ccgaccatca
     4081 gtaggctgcc gacgatcgcg tttgccagac cgccgccctc gctgcccggg gccggcgtag
     4141 tctcggtgaa cagggcaaag ctcagtgcgc cgaagccgcg caccagcaag gtgaacagga
     4201 tccaggcaag gaaaaacagg ccgatggcca ttgcgaacac cgacaaggcg atgccgatgc
     4261 ggtggaccca gagacggcgg cggtagacag ggttcatcat gacttggtcc cttccctgcg
     4321 cgacatgccg actagcatga acttggcggc ggccaacacg ataaaggtaa tgacaaacag
     4381 gatcagcccg agcgcgaaca gcgaagcaag atgcagctcc gattcggctt cggcaaattc
     4441 attggcgagc gtcgatgcga tgctgttccc aggagagaac agcgaccagg acaggaagtg
     4501 ggcgttgccg atcacgaagg tgaccgccat cgtttcgccc agcgcccgcc ccaggccgag
     4561 catcacgccg ccgaccacgc cggtgcgggt gtaaggcagc acgaccttgc gcaccacctc
     4621 ccatttcgtg cagcccaggc cataggccga ctccttcaac accgccggca cgatctcgaa
     4681 cacgtcacgc attaccgaag cgataaaagg aatgatcatc accgccagga tcagaccggc
     4741 ggtcaggatg ccgatgccca tcatcggccc ggtgaaaagc ggaccgatca gcggcacctg
     4801 gccgagcgtc gcagacaagg ccggctgcac gtagtcgccg aacagcggcg caaagatgaa
     4861 caggccccac atcccgtaga tgatcgacgg cacgccggcc aaaagctcaa ccgcggtgcc
     4921 gagtggacgc ttgagcgcgg tcggacagat ttcagtgaga aagacggcaa tgccgaagct
     4981 gatcgggaac gccaccagca aggcgatgat cgaggtcgcc aatgttccga cgatggcgat
     5041 cagcgcgccg tactggtcgt tgaccggatc ccactcgacg gtggtgacaa aggccgggcc
     5101 gaattccttg aacgcgggcc aggcgccgat catcagcgag gcgatgatgc ccaggagcac
     5161 gagcaacacg ctcaacgcga agatcaatgt gatcttgtgg aacagaaagt cctgcacccg
     5221 ctgcttgcgc acggtaaacg caagtgagcg ggcggcaatg gactggtttc ggtcttctag
     5281 ggtggtcgat tgcgtcacgg tcgatgtgcg ggcgcgtaag cccccggggg atgatggaca
     5341 ggcaaaacgg gttgcgcccg aatattatcc gaagatgaca ttccgggcga caacccctcg
     5401 atctgtcatg cgggtgtcat gcacccgcat gcttgggcgg cattactgca agatcggctt
     5461 gccttgcggg tcacgcagtt cagcgcgcca agcgtcctgc accagcttga ccaccgatgc
     5521 cggcatcggg acgtaatcca gttcagtcgc catcgcgccg ccgttcttga acgaccagtc
     5581 gaagaacttc agcacttcct gaccgcgggc cgggttctgc tgcaccttgt gcatcaggat
     5641 gaacgacgcg ccggtgatcg gccaggcacc cttgcccggc tggtcggtca gcacgatcgc
     5701 aaaaccgggc gtcttggccc agtccgcccc cgccgcggct gcctggaaac tcgcatcctc
     5761 gggctgaacg aactcacccg cccggttctg catctgcgcg tgggtgatat tgttgcgctt
     5821 cgcgtatgca acctcgacat acccgatcga gttgcggatg cgctgaacgt tggcggcaac
     5881 accctcgttg cctttcccgc ccatgccggt gggccacttc acggcagttc cggatccaac
     5941 cttttccttg aattccgggc tgaccttgga caggtagtcg gtgaacagga aagtggtgcc
     6001 tgacccgtcc gaacgataaa ccacattgat cggctgcgcc ggcagcttca atccctggtt
     6061 cagtgcggca atctcgggcg catcccagcg cttgatcgtg cccgtataga ttgcggcgag
     6121 cacggggccg gtcaggcgca gctgtcccgg cttcacgcct tcgacattca tgaccggcac
     6181 gacaccgccg atgatggccg ggaactgcac caggcccgaa tccgcgagct cctttgccgg
     6241 cagcggcatg tcggacgcgc cgaagtcgac ggtgcgcgcc ttgatctgct tgatcccgcc
     6301 gcccgagccg atcgactggt agttcaggct gaccccggtg gcattgcgat aggcctcggc
     6361 ccacttcgcg tagatcgggt acgggaaagt cgcgccggcg ccggtgatct gcgcgcttgc
     6421 ggaggtcgag atcgccagag cggcgaagcc gagtactgcc gatttcagga atttgctgag
     6481 ttgcatgtct gctcctaggg agttgtttgt cttcatggga cagggcgaac tctatccaac
     6541 aaatgtgaca gcttcatgac acagactggg acaggcgaaa taaagtgcca tgctccgagg
     6601 catgcaaaac aggcaagttc acgcagcgcg cctacttggt gccgaagatg cgatcccctg
     6661 cgtccccgag ccccggcagg atgtaggcat gctggtcgag cgcatcgtcg agcgaagcga
     6721 cgtacagccg cacgtcggga tgggcggcct ggaacacctc cacgccttcc ggagcggcaa
     6781 ccaaggcaag aaacaggatg cgttcgggcg cgacgccgcg cttgcgcagt acgtccaccg
     6841 catgcaccgc cgagttcccg gtcgcgacca ttgggtcgca caggatgaag gtgcgttcgt
     6901 gcaaatcggg cagtctgacc atgtattcga ccggctcgtg cgtctcgtga tcacggtaga
     6961 cgccgatgtg tcccacccgc gccgacggaa tcagctcgag cagaccgtcg ctcatgccga
     7021 tgcccgcacg cagcaccggc acgaccgcca gcttcctgcc ggcgattacc ggcgcgtcca
     7081 tcgcctgcat cggggtttcg atgcgccggg tggtcagcgg caattcccgc gtgacttcat
     7141 agcccatcag cagggtcagt tcacgcagca gttcgcggaa agtccgcgtg gacgtctccc
     7201 gatcgcgcat gtgggtcagc ttgtgctgga tcagcggatg atcgaggatg aagagattgg
     7261 gaaaacgcgg atcctgtttc atgggcggcg aaaaaagttg ggacggccgc cagtgtaacg
     7321 ctcaccacag caaacgcgat accgcgtgca ggcacaagcc gaccagtccg ccgaccagcg
     7381 tgccgttgat ccgtatgtac tgcag
//
LOCUS       DATLGF010000150         6166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_114482, whole genome shotgun sequence.
ACCESSION   DATLGF010000150 DATLGF010000000
VERSION     DATLGF010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6166
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114482"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="VM406_04520"
     CDS             complement(<1..1056)
                     /locus_tag="VM406_04520"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF045262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVL75258.1"
                     /translation="MNSALARGRSPFRFEPKIEARYEADTASRRNRELVGSGLVALLI
                     YNLFLINDYLIRPEMLSTTLGLRLGIMTPVGMFALWLIHRGQPPMLRETLIAATLPLA
                     MTLSALICYLSTSPFATYDPFSFSLIVLAGTTVFSLRFLHALASTAVSIAIMAAFLFI
                     DPKFVPELKGYALLVVVSTGIFTLIANYRLEASRRHSYLIARRESLRASAALEDNQAL
                     TRISLTDPLTRLPNRRQFEDSFAACWNQAAACDGSIGLLMIDVDHFKAFNDRYGHPVG
                     DRCLQQVAEALKAQVRADVDLVARIGGEEFVILLPGADTAAARQAGDRLRQAVEAIGI
                     AHESASDRPVVTISIGVA"
     gene            1318..1983
                     /locus_tag="VM406_04525"
     CDS             1318..1983
                     /locus_tag="VM406_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75259.1"
                     /translation="MGPIARTSPWRPSGAKRMAALMRCAAVEPESKHRPQGRSLQSEN
                     NRVSTARSKRWNGNVNALPIEFLEGVMEKMRAYGDRVQFSLLGGAGGPEYQVLNPLGK
                     AMAFDRHHHLLRPGDETFAGSNASPVYTCDQIQSIVAGNRVETGSQSKRPRVVRAATG
                     GTRASAARLAEQQAAERYAYFKENRAMLPATITQHTAEIDAHMKAGKPVAEAFDAVVR
                     KYY"
     gene            complement(2169..2900)
                     /locus_tag="VM406_04530"
     CDS             complement(2169..2900)
                     /locus_tag="VM406_04530"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HVL75260.1"
                     /translation="MRYELYYWPSIQGRGEFVRLALEDAGADYVDVARRAERNGMGMS
                     AMMAYLEGDETACPPFAPPFLKAGELVIAQTANILFFLGPRLGLAPDDEAGRLWVHQL
                     QLTIADMVTEVHDTHHPIGSGLYYEEQKKEAARRSADFIGARLPKFFDYFETALARNP
                     CGPVWMAGDRPSYADLSMFQMIAGLRYAFPRTMERIEPDYPLLVALHDGVAARPRIKA
                     YLKSKRRIPFNNDDIFRHYPELDSG"
     gene            complement(3016..3399)
                     /locus_tag="VM406_04535"
     CDS             complement(3016..3399)
                     /locus_tag="VM406_04535"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HVL75261.1"
                     /translation="MKHILCITALVLGSAALAPAVAAPQIGIHVTVGTPPPPVRYEAA
                     PRPHRGHEWIPGYWDWTGRRYVWVPGRWEKARAGHYYRRAEWRQGPRGWTLVRGGWQA
                     HPRPHHVRTPPPPRAYTPHSHGPRR"
     gene            3641..3955
                     /locus_tag="VM406_04540"
     CDS             3641..3955
                     /locus_tag="VM406_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75262.1"
                     /translation="MIIARWSIDARFGHKQAVIDSMIRWQKEIGTQIGWNEMRLLTGS
                     VGARESTVQSEVRLNSLAELSEAWDKLGTIDAHKQWSKELEPNIVSGTPRWEVFRVLA
                     EK"
     gene            complement(4022..4165)
                     /locus_tag="VM406_04545"
     CDS             complement(4022..4165)
                     /locus_tag="VM406_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75263.1"
                     /translation="MSRALPPGMPDRLDVSIVRSEQGVERSTSLVYGRHTYLIHYEFR
                     PAG"
     gene            complement(4351..5088)
                     /locus_tag="VM406_04550"
     CDS             complement(4351..5088)
                     /locus_tag="VM406_04550"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HVL75264.1"
                     /translation="MFALLLLSFGVLAILAGRVDRARRLARFFARQMLRLGGLPVSVS
                     GLDRLPPAPHVLLANHTSFLDPIVLVACLPPVPGYTYTTRRQHLLQVLLWPFLHSLHT
                     LILKPHTSGESGDHHHSVNVERMEAVLRRGDNLMVFPEGAFRPEPGLAPFHSGAFVAA
                     AQAQVPIVVVGLRGARDALRLGTWRPRRASLALEIGTVLQPSGSDPRAIHALMDEAYA
                     AMVPLTGEPPRAGVHGWAHADDAHRRA"
     gene            5289..6050
                     /locus_tag="VM406_04555"
     CDS             5289..6050
                     /locus_tag="VM406_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL75265.1"
                     /translation="MRAQLTYRLKRLNRSLRARLCLFSSRFDALGAPSPLLKLEYAAH
                     KESRTLIIFLPGIDDLAEDFERRGFIHELRRAGVAADAIAVDAHFGYYARRVILERVT
                     EDVIASAHASGYEEIWLVGISLGGFGAASYAARHPSHVNGVLLLAPYLGDKPLIDEIV
                     TAGGLDAWDPGQVTHADYPRSLWSWLKRHPSMPGAAPPIYLGYGTGDAFARANALLAD
                     VLPERHVFAISGRHDWHTWMQIWRMFLGEWGAHRR"
BASE COUNT         1118 a         1924 c         2069 g         1055 t
ORIGIN      
        1 ggcaaccccg atgctgatgg tgacgaccgg acgatcggac gcgctttcat gtgcaattcc
       61 gatggcctcg accgcctggc gcagccgatc gccggcctgc cgagcggcgg cggtatcggc
      121 gccagggagc aggatgacga attcttcacc gccgatgcga gcaaccagat cgacgtcggc
      181 gcgcacctgt gccttcagcg cttcggcgac ctgctgcagg cagcggtcgc cgaccgggtg
      241 gccgtagcgg tcgttgaagg ccttgaagtg gtcgacgtcg atcatcagca atccgatcga
      301 accgtcgcat gcggcggcct ggttccagca ggcggcaaag ctgtcttcga actggcgccg
      361 gttcggcagg cgcgtcagcg gatcggtcag cgagattcgc gtgagcgcct ggttgtcttc
      421 gagggcggcg ctggcgcgca ggctttcacg ccgcgcgatc agataggagt ggcgccggct
      481 ggcttccagc cggtagttgg cgatcagcgt gaaaatgccc gtcgagacga cgaccagcag
      541 cgcatagccc ttgagctccg gcacgaactt ggggtcgatg aagaggaacg ccgccatgat
      601 ggcgatgctg acggcggtgg acgccagcgc gtgcagaaag cgcagggaaa acacggtcgt
      661 cccggccagc acgatgaggc tgaacgaaaa cggatcgtaa gtcgcgaacg gcgaggtcga
      721 gagatagcag atcagcgcag acagcgtcat ggccagcggg agcgtcgccg cgatcagggt
      781 ctcgcgcagc ataggcggct ggccgcgatg gatcagccaa agcgcaaaca tcccgaccgg
      841 cgtcatgatc cccagtcgca ggcccagcgt ggtcgacagc atctcgggcc ggatcaggta
      901 atcgttgatc aggaacaggt tgtagatcag cagcgcgacc agtcccgaac cgaccagctc
      961 ccggttgcgc cgggatgcgg tatcggcttc gtagcgcgcc tcgatcttcg gctcgaagcg
     1021 gaacgggctg cgcccccggg ccaaggcaga attcacggcc tgaagttcgc cggccaaaaa
     1081 agaagcctcg ccaggtacgc gctgcatatc aaaccgcctt catcccgtgt atttctctga
     1141 ggtaatacta acagtaacaa caacgaagaa gcgagaggcc gatccgtcca cgcgaaaaaa
     1201 tggtcgagcg ctcggaaaat gaatcaggag cggcgcgtgc ccgctgcgcg gccggaagcg
     1261 cttgtgcccg atcaaaggct ttcaggctgt cctgcatcag cataggtggg gagaacaatg
     1321 gggccgatcg cgcgtacgtc gccgtggcgg ccttccggcg cgaagcggat ggcggcactc
     1381 atgcgctgcg ccgcagtcga accagaatcg aaacatcgcc cgcaggggcg ttccttacag
     1441 agcgagaaca acagagtgag tacagcaaga agcaagcgtt ggaacggcaa tgtcaatgcc
     1501 ctcccgatcg aattccttga aggcgtcatg gagaaaatgc gcgcctatgg cgaccgcgtg
     1561 cagttttcac tgctcggcgg cgccggcggg cctgagtatc aggtgctcaa cccgctcggc
     1621 aaggccatgg cgttcgatcg ccaccaccat cttctgcgtc ccggcgatga gaccttcgcc
     1681 ggcagcaacg cgagtcccgt ctatacgtgc gaccagatcc agtcgatcgt tgccggcaac
     1741 cgcgtcgaga cggggtcgca gtcaaagcgg ccgcgggtcg tacgggccgc cacgggcggc
     1801 acccgtgcga gcgccgcgcg gctggccgaa cagcaggcgg ccgagcgcta tgcgtatttc
     1861 aaggaaaacc gcgccatgct gcccgcgacg atcacgcagc acacggccga gatcgatgcg
     1921 cacatgaagg ccggcaagcc ggtcgccgag gcgttcgatg cggtggtgag gaagtactac
     1981 tgagggggtg gagcggtagg acgagcacgc cgtccgtgcc aggggtgtct ggtgctggta
     2041 ttgctttccg accgccagct ccggtggacg cagcctgttc ttttgaaaac aatgtctcac
     2101 tccgacggct gcatgcctgc catgttacgc attgacgcgg ggaacaggtg ttactttgcg
     2161 taacctgcct acccgctgtc cagctcgggg tagtgccgga agatgtcatc gttgttgaac
     2221 gggatgcgcc gctttgattt caggtacgcc ttgatgcgcg gccgtgccgc cacgccgtcg
     2281 tgcagggcga cgagcagcgg atagtcgggc tcgatgcgct ccatcgtgcg cgggaatgcg
     2341 tagcgcaggc cggcgatcat ctggaacatc gacagatcgg catagctcgg cctgtccccc
     2401 gccatccaca ccggcccgca gggattgcgc gcgagcgcgg tctcgaagta gtcgaagaac
     2461 ttcggcagcc gcgcgccgat gaagtccgcg ctgcgccgtg cggcctcctt cttctgttcc
     2521 tcgtagtaga gcccgctgcc gatggggtgg tgggtgtcgt gcacctcggt caccatgtcg
     2581 gcaatggtca gctggagctg gtgcacccac agccggccgg cctcgtcgtc cggcgcgagg
     2641 cccaggcgag gtccgagaaa gaacaggatg ttggccgtct gcgcgatgac gagctcgccg
     2701 gctttcagga atggcggcgc gaacggcggg caggcagttt cgtccccctc gaggtacgcc
     2761 atcatcgccg acatgcccat gccgttgcgc tcggcgcggc gcgccacgtc gacgtagtcc
     2821 gcgccggcgt cttccagcgc gagccgcacg aattcgccgc gtccctggat ggatggccag
     2881 tagtagagct catagcgcat gcggcacctc gtcgaagtcc tgttttctgc ctttttccgg
     2941 cctcagccat gagaccggca tctcctgcgg ccattcccga cttgcctgca gcgatcgcgc
     3001 accatcatgc gcgactcagc ggcgcggacc atgggaatgt ggagtgtagg cacgcggagg
     3061 aggcggcgtg cgcacatggt gcggtctcgg gtgggcctgc catccgcctc gcaccagcgt
     3121 ccagccgcgc ggcccctggc gccattccgc gcgccgatag taatggcccg cgcgcgcttt
     3181 ttcccagcgg ccaggcaccc agacgtagcg gcgcccggtc cagtcccagt aacccggaat
     3241 ccattcatgg ccgcgatgcg ggcgtggcgc cgcctcgtag cgcaccggcg gcggcggtgt
     3301 gccgaccgtg acgtggatgc cgatctgcgg tgcggccacg gccggcgcga gagcggcgct
     3361 gccaagaacg agtgcggtga tgcagagaat gtgtttcatg tcgatctccg ttcgggtagg
     3421 tgggatcgac tatagagagc gcatgcctgg caacaaactg ccgatgcaag ttctttcacg
     3481 gaaaggcaga gcgatacatt gcgaaatgcg catgcacaga agcgtttgag gcggaacaag
     3541 ccggcagggg gcgtgctgcc tatagtgggc tgagaccggc ttttccggcg cgggtgtgcg
     3601 gcatggcgcc acgcgccggt ttcttcacgg gagaccagcc atgatcattg cacgctggag
     3661 catcgatgcc cgcttcgggc ataaacaggc cgtgatcgat tccatgatcc ggtggcagaa
     3721 ggaaatcggc acacagatcg gttggaacga gatgcggctg ctgaccggtt cggtcggcgc
     3781 gcgcgagtcg acagtccagt ccgaggtgcg cctgaacagc cttgccgaac tgagcgaggc
     3841 atgggacaag ctcggcacca tcgacgcgca caagcagtgg agcaaggaac ttgagcccaa
     3901 tatcgtttcc ggtacgccgc gctgggaagt attccgcgtg ctggcggaga aataacccgt
     3961 cgcaccatcc gaccgccacc accggctggt ttgaagatct ccgccggcgc ggggccgctc
     4021 atcatccggc cggccggaat tcgtagtgga tcagataggt gtgccggcca tagacgagcg
     4081 acgtggacct ttccacgcct tgctcggaac gcacaatgga gacatccagg cgatccggca
     4141 tgcccggcgg gagggcacgc gacatgcggg cgtaatcgcg ccgcgagatc tgcgcaggca
     4201 tcacccattc gcgcgccggc gcagcgttgc gctcgatggg tgccgatggt ggcgaaggcg
     4261 gaaggattcc tgacataagg caacaagctt ggcgaccgta gggctttgaa agccttgcac
     4321 cgtcttcgtt ccgcacggct ttcgttccat tcatgcgcgg cggtgtgcat catcggcatg
     4381 ggcccagcca tgtacgccgg cgcgcggcgg ctctccggtg agcgggacca tcgccgcata
     4441 tgcctcgtcc atcagtgcgt gtatggctcg cgggtcgctg cctgatggct gcagcaccgt
     4501 gccgatctcg agcgcaagcg atgcgcggcg cggcctccac gtccccaggc gcagcgcatc
     4561 gcgcgcgccg cgcagcccga cgacgacgat tggaacctgc gcctgcgccg cggcgacgaa
     4621 tgcgccggag tgaaacgggg ccaggccggg ctcgggccgg aacgctccct ccggaaacac
     4681 catcaggttg tctccccgcc gcagcaccgc ctccatccgc tccacgttca cgctgtggtg
     4741 atggtcaccg ctctcgccgc tggtgtgcgg cttgagtatc agggtatgca ggctgtgaag
     4801 aaaaggccac agcaggacct gcagcaagtg ttgccggcgc gtcgtgtacg tgtatcccgg
     4861 caccggcggc aggcaggcga ccagaacgat gggatcgagg aagctggtgt gatttgccag
     4921 aaggacatgc ggtgccggcg gcaggcgatc cagaccgctg acggacaccg gcagtccgcc
     4981 caggcgcagc atctgacgtg cgaagaagcg tgccagtcgg cgcgcgcggt ctacccggcc
     5041 tgcgaggatt gcgagcaccc cgaacgagag cagcagcagc gcgaacatga cccaggcgta
     5101 gcagctgtac gcccaggcgc cggcgcgaga ggtctgcggg tcgggagctt gcgtcatctg
     5161 tgtcaggcat ccaggcggca gtcgtggcag tgtgcaaccg cgcagcgcgc gcgggctcaa
     5221 agggagcagg cggcgcgtgc aacaaggtgg cactgccgct cgcatatggc gtgccaggaa
     5281 ccaatcccat gcgtgcccag ttgacctatc gcctgaagcg cctgaaccgc agcctgaggg
     5341 cgcggctatg cctgttttcg tcccgcttcg atgcgctcgg cgcaccgtcg ccactgttga
     5401 agcttgagta tgcggctcat aaggagagcc gcacgctgat catctttctc cccggtatag
     5461 acgacctggc cgaggacttc gaacggcgcg gattcatcca cgaactgcgt cgcgcgggcg
     5521 tagctgccga tgcaattgcg gtcgacgcgc atttcggtta ctacgcccgg cgcgtgattc
     5581 tcgagcgcgt caccgaggat gtgatcgcct cggcccatgc gagcgggtat gaggagatct
     5641 ggctggtcgg gatttccctt ggcggattcg gcgcagcttc ctacgcggcc cgccatccgt
     5701 cgcatgtcaa cggggtcctg ctgctcgcgc cttatctggg cgacaagccg ctgatcgatg
     5761 agattgtcac agcaggcgga ctggacgcat gggatcccgg acaggtcacg catgcggact
     5821 atccgcgcag cctgtggagc tggctgaaac ggcatccttc catgcccggg gccgcgccgc
     5881 cgatctatct tggctacggt accggtgatg cgttcgcgcg cgccaacgcg cttctcgccg
     5941 acgtgcttcc cgagcggcat gtctttgcga tctccggccg ccacgactgg catacctgga
     6001 tgcagatctg gcggatgttt ctcggcgagt ggggcgcgca tcggcgctga gaacggcgcg
     6061 cggcgtcagg tgttgctttt gcgtcagttc tgccgcttcc atgcattttt tccatttatc
     6121 gcggaccaca ccctgattac aatcttgcct ttcagaaaca agaccc
//
LOCUS       DATLGF010000151         6554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_85071,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000151 DATLGF010000000
VERSION     DATLGF010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85071"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1663)
                     /gene="aceK"
                     /locus_tag="VM406_04560"
     CDS             complement(<1..1663)
                     /gene="aceK"
                     /locus_tag="VM406_04560"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HVL75266.1"
                     /translation="MRDVFPKLLSSQIAFDIARTILDGFNKHYRLFRAASQDARRYFE
                     TRDWKTAQVKARERIAFYDTRVQECVQALEDEYDEEDLTDDVWREVKLHYIGLLLGHK
                     QPELAETFFNSVCCNILHRSYYHNDFIFVWPAIATDYIEPDEDRPTYRVYYPAKDGLR
                     YTLKRIITNFQLQGEFADLDRDVGFVETRMRTMFGSELLEPNHQIQVLATLFYRNKGA
                     YIVGKGINGNRVYPFVVPILTNRKNELVLDTVLFDPTLITVLFSFTRAYFMVDMEVPS
                     AYVRFLRTLMPYKPRSEIYTILGLQKQGKAAFYRDFLHHLRYSSDCFEAAPGIRGLVM
                     VVFALPSFPYVFKVIKDYFPPPKETTRALVKEKYLLVKNHDRVGRMADTLEYSSVAFP
                     RARFSDALLEELKEVAPSIVEEDGDEVIIKHLYIERRMVPLNIYLSEAEKRGDHAAVE
                     HGIVEYGNAIRDLVAVNIFPGDMLYKNFGVTKHGRVVFYDYDEIEYITDCNFRTIPAP
                     RNEEDEMSAEPWYPVGKNDVFPEQFGTFLLGHPEVRKYFLKHNADL"
     gene            complement(1656..2591)
                     /locus_tag="VM406_04565"
     CDS             complement(1656..2591)
                     /locus_tag="VM406_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HVL75267.1"
                     /translation="MHPLHIATPLLTHPALSARLGKQVLLKMENLQPSGSFKLRGIGA
                     MCQRAVQAGARLLVCPSGGNAGAAAAVAGAALGVPVVIVVPVTTHVSVQDKLRQFGAR
                     VQVHGDVWDEANSFALQLCEQQGGAYVPPFDHPDIWDGNATMIDEALAQGARFDAVVC
                     SVGGGGLMCGVLEGLHRNGMGHVPLLAVETEGAASLRAAIQAGELVTLPAITSLATSL
                     AAKRVAAAALEWTRRHDVRSVVVSDRQALDACVAFADDQRALVEPACGAALAVAYGNL
                     PVLREFERPLVIVCGGLAVDLAKLDGWRTSESRHA"
     gene            complement(2594..3031)
                     /locus_tag="VM406_04570"
     CDS             complement(2594..3031)
                     /locus_tag="VM406_04570"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HVL75268.1"
                     /translation="MMAFETYRGFVYPASLDHIGHMNVQFYTGRFDEATWHLFARLGI
                     TQTYLKANKRGMAALDQHTQYKREVLVGSVLHITSELLEIKRKTVRFMHTMINSETGE
                     VVATTELVAAFLDAEARRATEFPPEILDAALPLLRTEAPASSA"
     gene            complement(3028..3462)
                     /locus_tag="VM406_04575"
     CDS             complement(3028..3462)
                     /locus_tag="VM406_04575"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HVL75269.1"
                     /translation="MHTPNPHYRDTIAASFREAQFLVDLGIRLVDCGPGWCETVLELA
                     PRHCQHHGFVHAGVQATIADHTAGAAAMSVTPEGSTVLTSEFKIHLLRPGKGERLTAR
                     AQVLKPGRMFHVVECEVFALTGDERSLVSKLSATMAVVERSA"
     gene            complement(3630..4814)
                     /locus_tag="VM406_04580"
     CDS             complement(3630..4814)
                     /locus_tag="VM406_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HVL75270.1"
                     /translation="MIHLPGLTFDHGEDIAALRETVQQFAQAEIAPRAAEIDRSDQFP
                     MDLWKKMGDLGLLGVTVDEEYGGTNMGYLAHIVAMEEISRASASVGLSYGAHSNLCVN
                     QIKRNGTEEQKQKYLPKLISGEHVGALAMSEPNAGSDVVSMKLRADWKGDRWVLNGTK
                     MWITNGPDADVLVVYAKNDIEAGPRGMTAFLIEKGFPGFSVAQKLDKLGMRGSHTGEL
                     VFENCEVPAENVLGGLGKGVNVLMSGLDFERTVLSGGPLGIMQACMDAVVPYVHDRKQ
                     FGQPIGEFQLMQGKLADMYSTMMACKAYVYAVGQACDRAKTPEQVRALRKDAAGAILY
                     SAEKATWMAGEAIQALGGNGYINEYPVGRLWRDAKLYEIGAGTSEIRRMLIGRELFAE
                     TR"
     gene            complement(4942..5379)
                     /locus_tag="VM406_04585"
     CDS             complement(4942..5379)
                     /locus_tag="VM406_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013337847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HVL75271.1"
                     /translation="MPTYTITELAREFDITPRAIRFYEDQGLLAPKREGAGGRTRVYS
                     ARERTRLKLTLRGKRLGLSLSEIKSLVDMYESPKDTAAQLKRFQAVLAQHRETLERQR
                     EDIEVTLAEIAAHEVECRRLLEMERPAKAGEKKSGARKLKDAA"
     gene            5507..6550
                     /locus_tag="VM406_04590"
     CDS             5507..6550
                     /locus_tag="VM406_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVL75272.1"
                     /translation="MNPLESQLEYALADLLPDAGVPHEVAPGVFWLRMRLPFALDHIN
                     LWLLRDGNGWTVVDCGIATDETRAAWEQVFATGLQGLPITRVLVTHCHPDHVGLADWL
                     CERWQAPLWMSAGEYAFARMMSASLPGSDGTAMFPHFQRHGLTDPAMQERLQARKSYY
                     TNLVPAVPIAYRRIREQDVVDIGGRGWRVITGFGHSPEHVSLYCAELGVLISGDMLLP
                     RISTNVSVFAIEPEANPVQLYLDSLQAYLPLPADTLVLPSHGKPFRGAHTRVAQLQAH
                     HAERLDEVLDACATPKSAADIVPVMFKRPLDAHQLTFALGEALAHLHKLWFDGALQRE
                     HGEDGIIRFCRTA"
BASE COUNT         1109 a         2160 c         2087 g         1198 t
ORIGIN      
        1 gcagatcggc gttgtgcttc aggaagtact tgcgcacttc cgggtgcccg agcaggaagg
       61 tgccgaactg ctcggggaag acgtcgttct ttccaaccgg ataccagggc tcggccgaca
      121 tctcgtcctc ttcgttgcgc ggtgccggaa tcgtgcggaa attgcagtcg gtgatgtact
      181 cgatctcgtc gtagtcgtag aacaccacgc ggccatgctt ggtcacgccg aaattcttgt
      241 acagcatgtc gccggggaag atgttgaccg cgaccagatc gcggattgcg ttgccgtact
      301 cgacgatgcc gtgctcgacg gccgcatggt cgccgcgctt ttccgcttcg ctcaggtaga
      361 tgttcagcgg caccatgcgc cgctcgatgt acaggtgctt gatgatcacc tcgtcgccat
      421 cctcctcgac gatggacggc gccacttcct tcagttcctc cagcagcgcg tcggaaaagc
      481 gcgcgcgcgg aaacgccacg ctcgagtact ccagcgtgtc ggccatgcgg ccgactcggt
      541 cgtggttctt cacgagcagg tacttttcct tgaccagcgc gcgcgtcgtc tccttcggcg
      601 gcgggaagta atccttgatc accttgaaca cgtagggaaa ggacggcagc gcaaacacca
      661 ccatcaccag cccgcgtatg ccgggcgcgg cctcgaagca gtcggatgaa tagcgcaggt
      721 gatggaggaa gtcgcgatag aacgccgcct tgccctgctt ctgcaggccg agaatcgtgt
      781 agatctcgct cctcggcttg tacggcatca gcgtgcgcag gaaacgcaca taggccgacg
      841 gcacttccat gtcgaccatg aagtaggcgc gcgtgaacga gaacagcacc gtgatcagcg
      901 tcggatcgaa caacacggtg tcgagcacca gctcgttctt ccggttggtc aggatcggca
      961 ccacgaacgg atagacgcgg ttgccgttga tgcccttgcc gacgatgtag gcgcccttgt
     1021 tgcggtagaa cagcgtggcg agcacctgga tctggtggtt cggctccagc agttccgagc
     1081 cgaacatcgt gcgcatgcgc gtctcgacaa agccgacgtc gcggtcgaga tcggcgaact
     1141 cgccttgcag ctggaaattc gtgatgatgc gcttcaacgt atagcgcagc ccgtccttgg
     1201 ccggatagta aacacgatag gtcggcctgt cctcgtccgg ttcgatgtaa tcggtggcga
     1261 tcgccggcca gacgaagatg aaatcgttgt ggtagtacga gcggtgcagg atgttgcagc
     1321 agaccgaatt gaagaaggtc tcggccagtt ccggctgctt gtggccgagc agcaagccga
     1381 tgtagtgcag cttgacctcg cgccagacat catcggtcag gtcctcctcg tcatactcgt
     1441 cctccagcgc ctgcacgcat tcctgcaccc gcgtgtcata gaacgcgatg cgctcgcgcg
     1501 ccttgacctg cgcggtcttc cagtcgcgcg tctcgaaata gcggcgcgca tcctggctgg
     1561 ccgcgcggaa cagccggtag tgcttgttga agccgtccag gatcgtgcgc gcgatgtcga
     1621 acgcgatctg ggacgagagc agcttgggga agacgtcacg catggcggga ttccgaggtt
     1681 ctccagccgt ccagtttagc caaatccacc gccagcccgc cgcagacgat gacgagcggg
     1741 cgctcgaatt cccgcagcac gggcaggttc ccgtaggcaa cggcaagtgc cgcgccgcag
     1801 gcgggctcca ccagggcacg ctggtcgtcg gcgaacgcga cacaagcgtc gagcgcctgc
     1861 cggtccgaca ccaccacgct gcgcacatcg tgacgtcggg tccattccag cgccgccgcc
     1921 gccacgcgct tggcagccag ggaggtcgcc agcgaggtga tcgccggcag cgtgaccagc
     1981 tcgccagcct ggattgccgc acgcagcgat gcggcgccct cggtctctac cgcgagcagc
     2041 ggaacatgtc ccatgccgtt gcggtgcagg ccttcgagca cgccgcacat cagcccgccg
     2101 ccgccgaccg agcagaccac cgcatcgaag cgtgcaccct gcgcgagcgc ttcatcgatc
     2161 atcgtcgcgt tgccgtccca gatgtccgga tggtcgaacg gcggcacgta ggcgccgccc
     2221 tgttgctcgc aaagctgcag cgcaaagctg ttggcttcgt cccagacgtc gccgtgcacc
     2281 tgcacgcggg cgccgaactg gcgcagcttg tcctgcaccg agacatgggt ggtcaccggc
     2341 accacgatca ccaccggcac gccgagcgca gccccggcca cggccgcggc cgccccggca
     2401 ttgccgccgg acgggcagac caggaggcgc gcgccggcct gcaccgcgcg ctggcacatc
     2461 gcgccgatgc cgcgcagctt gaacgaaccc gacggctgca ggttttccat cttcaagagc
     2521 acctgcttgc ccaggcgggc ggacaaagcc gggtgggtca gcagcggcgt ggcgatgtgg
     2581 agtggatgca tggctatgcg cttgaggcgg gcgcttcggt gcgcaacagg ggcagcgcgg
     2641 cgtccagaat ttccggcgga aattccgtgg cgcggcgggc ttcggcatcg aggaatgcgg
     2701 cgaccagctc ggtcgtggcg accacttctc ctgtttcact gttgatcatc gtgtgcatga
     2761 agcgcaccgt cttgcgcttg atctcgagca gctcgctcgt gatatgcagc accgagccta
     2821 ccaacacctc gcgcttgtac tgcgtgtgct gatccagcgc cgccatgccg cgcttgttcg
     2881 ccttcagata ggtctgcgta atccccaacc tggcgaacag gtgccaggtc gcttcatcga
     2941 aacggccggt gtagaactgc acgttcatgt gcccgatatg gtcgagcgac gcgggataga
     3001 cgaagccgcg ataggtttcg aacgccatca tgcactcctt tcgacgacgg ccatcgtcgc
     3061 gctcagcttg ctgacgaggc tgcgctcgtc gccggtgagc gcgaacacct cgcactcgac
     3121 cacgtggaac atgcggccgg gcttgagtac ctgcgcgcgc gccgtcagcc gctctccctt
     3181 gcctggacgc agcagatgga tcttgaattc ggaagtcagc accgtgctcc cctcgggcgt
     3241 gacgctcatc gccgcggcgc cggcggtgtg gtcggcgatg gtcgcctgca cgcccgcatg
     3301 cacgaagccg tggtgctggc aatggcgcgg cgccagttcc agcaccgtct cgcaccaccc
     3361 cggtccgcag tcgaccaggc ggatgccgag gtcaacgagg aactgcgcct cgcggaagga
     3421 ggcggcgatg gtgtcgcggt agtgggggtt cggcgtatgc atgagccttc ctgtttgaac
     3481 aacgacggcc ggttccttcc ccctggaagg gggaaggcca ggatgggggc gtggacccga
     3541 tgctggcaac gacattcgat tgctctgcgc accaccaccc tgaccctccc ctcaaaggcg
     3601 gagagaaccc gacagcggaa gaacgacgat taccgcgtct ccgcaaacaa ctcccgcccg
     3661 atcagcatcc ggcggatctc gctggtgccg gcgccgatct catacagctt cgcatcgcgc
     3721 cacaggcggc cgaccgggta ttcgttgatg tagccgttgc cgccaagcgc ctggatcgcc
     3781 tcgccggcca tccaggtcgc cttctcggcc gagtagagga tcgcaccggc ggcgtccttg
     3841 cgcagcgcgc gcacctgctc gggcgtcttc gcgcggtcgc aggcctggcc caccgcgtag
     3901 acgtaggcct tgcaggccat catcgtcgag tacatgtctg ccagcttgcc ctgcatcagc
     3961 tggaactcgc cgatcggctg gccgaactgc ttgcggtcat gcacgtaagg caccaccgca
     4021 tccatgcagg cctgcatgat gcccagcggc ccgccggaga gcaccgtgcg ctcgaagtca
     4081 aggccggaca tcagcacgtt gacacccttg ccgagaccgc ccagcacgtt ctcggccggc
     4141 acttcgcagt tctcgaacac cagttcgccg gtgtgcgagc cgcgcatgcc gagcttgtcg
     4201 agcttttgcg caacggaaaa gccggggaaa cccttctcga tcaggaaggc cgtcatgccg
     4261 cgcgggccgg cttcgatgtc gttcttcgca tagacgacca gcacatccgc gtccgggccg
     4321 ttggtgatcc acatcttggt gccgttcaac acccagcgat cgcccttcca gtcggcgcgc
     4381 agcttcatgc tgaccacgtc cgagccggca ttcggctccg acatcgccag cgcgccgaca
     4441 tgctcgccgg agatcagctt gggcaggtac ttctgcttct gctcctcggt gccgttgcgc
     4501 ttgatctggt tcacgcacag gttcgagtgc gcaccgtagg acaggccgac cgaagccgag
     4561 gcgcgcgaga tctcctccat cgcgacgatg tgcgcgaggt agcccatgtt ggtgccgccg
     4621 tattcctcgt cgacggtgac gcccagcagg cccaggtcgc ccatcttctt ccacaagtcc
     4681 atcgggaact ggtcgctgcg gtcgatctcg gcggcgcgcg gggcgatttc ggcctgggca
     4741 aactgctgca cggtttcgcg cagggcggcg atgtcctcgc catggtcgaa tgtcaagccg
     4801 ggcaggtgaa tcatgtcgtc ctctcgttat cgtaggggtt gggggccagc ggttagggcg
     4861 aaacggcccg atgtgcgaat aaatgatacg gtctatttac gtttacgtaa acgtaaagca
     4921 gatacggtaa aaataccgcg ctcaggcggc gtccttcagc ttgcgcgcgc cgctcttttt
     4981 ctcgcccgcc ttcgccggcc tttccatctc gagcaggcgc cggcactcga cctcgtgcgc
     5041 tgcgatctcg gccagcgtca cctcgatatc ctcgcgctgg cgttcgagcg tctcacggtg
     5101 ctgggcgagc acggcctgga agcgcttcaa ttgtgccgcg gtatccttcg gcgactcgta
     5161 catgtcgacc agactcttga tttccgacag ggacagcccg aggcgcttgc cgcgcagggt
     5221 gagcttgaga cgggtgcgtt cgcgcgcgct gtagacccgt gtgcgcccgc ccgcgccttc
     5281 gcgcttgggc gcaagcagcc cctggtcttc gtagaagcga atggcgcgcg gcgtgatgtc
     5341 gaattcacgg gccaattcgg tgatcgtgta ggtgggcatg aggctcgctc ggcagtggat
     5401 acggattgcg tttagaatcg ccggtatgtc ttgcagttta cgttaacgta aactgcattc
     5461 gtattgtagg cccgcgccgg ccgcttgtga atggcccctc gaaagaatga atccgctcga
     5521 atcgcaactc gaatacgccc tggccgacct gctgcctgac gccggcgtgc cgcacgaagt
     5581 cgcccccggg gtgttctggc tgcgcatgcg cctgccgttc gcgctggacc acatcaacct
     5641 gtggctgctg cgcgatggca atggctggac ggtggtcgac tgcggcatcg cgaccgacga
     5701 aacccgtgcc gcgtgggaac aggtgttcgc gactgggctg caggggctgc cgatcacgcg
     5761 tgtgctggtg acccactgcc atccggacca cgtcggcctg gccgactggc tgtgcgagcg
     5821 ctggcaggcg ccgctgtgga tgagcgccgg cgaatacgcg ttcgcgcgga tgatgtcggc
     5881 atcgctgccc ggctccgacg gcaccgcgat gttcccgcac ttccagcgcc atggcctgac
     5941 cgatccggcg atgcaggagc gcctgcaggc ccgcaagagt tattacacga acctggtgcc
     6001 ggctgtgccg atcgcctacc gccgcattcg cgaacaagac gtcgtggaca tcggcgggcg
     6061 cggctggcgg gtcatcaccg gcttcggcca ttcgcccgag cacgtgtcac tgtactgcgc
     6121 ggagcttggc gtcctgattt ccggcgacat gctgctgccg cgcatttcaa caaacgtttc
     6181 ggtgttcgcg atcgaacccg aggccaatcc cgtccagctc tacctggact cgctgcaggc
     6241 ctacctcccg ctgcctgcgg acacgctggt gctgccctcg cacggcaagc cgtttcgcgg
     6301 cgcgcacacc cgcgtcgccc agctgcaggc ccaccacgcc gagcggctgg acgaagtgct
     6361 ggacgcctgc gcaacgccga agagcgccgc cgacatcgta ccggtgatgt tcaagcgccc
     6421 gctcgacgcc caccagctca cgttcgcgct cggggaggcg ctcgcccacc tgcacaagct
     6481 gtggtttgac ggcgcgctgc agcgtgagca tggcgaggac ggcatcatcc gcttttgccg
     6541 caccgcctga ccgg
//
LOCUS       DATLGF010000152         4398 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_22718,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000152 DATLGF010000000
VERSION     DATLGF010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22718"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VM406_04595"
     CDS             complement(<1..762)
                     /locus_tag="VM406_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HVL75273.1"
                     /translation="MSRIERRVLLERFQDMVRRREPIVGAGAGTGISAKCEEAGGADL
                     IIIYNSGRYRMAGRASSAGLLAYGNANQIVVEMAGEVLPVCKRTPVIAGVNGTDPFII
                     MPVFLKQLKEMGFSGVQNFPTVGIIDGTFRQSLEETGICFASEVDMIRQAHELDLLTT
                     PYVFSADEAVEMAKAGADLIVPHMGVTTGGTIGATTAKSLDESVRLIDEWGEAARRIR
                     KDVIIIAHGGPIATPEDVEYVLKRSEHCNGFYGASS"
     gene            complement(759..1763)
                     /locus_tag="VM406_04600"
     CDS             complement(759..1763)
                     /locus_tag="VM406_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVL75274.1"
                     /translation="MMRATLRVAAILGLLLLLAVPWMPTVVYPVFVMKLFCMALFACA
                     YGLLLGFGGMMSFGHAAFFGGAAYLTGVATKHWGLPTELGLVVGMASAALLGLVIGGL
                     AIRRQGIYLAMITLALAQMVYFIFLQAPFTGAEDGMQNIPRGSLLGVIDLRSDVAMYY
                     LVMTLTALGLWIIHRVVHSPFGHVIVAIREHEPRAVSLGYPVARYKLMLFVLSAALSG
                     LAGSLKALVFGLAALTDVHWHLSGDVVLMALLGGMHTVFGPAVGAALVVGLQFYLDAL
                     GGWVTLITGIVFMVCVLSFRKGIVGTLIELAERRAARGAADAVRGSGSATLQNRESSI
                     "
     gene            complement(1760..2653)
                     /locus_tag="VM406_04605"
     CDS             complement(1760..2653)
                     /locus_tag="VM406_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVL75275.1"
                     /translation="MSEVLGMPLPVLAGQTVLGLVNGCFYAVLSLGLAVIFGMLKIVN
                     FAHGAFFMLGGFGAWMLLTYVGGNYWLALILVPLIVGALGGVFERSFIRRLYGLDPIY
                     GLLLTFGLVLALEGGFRVAYGTSGKPFPVPEALRGVFDMGFMVMPLYRIWVIVAALLA
                     CLGTWWLIERTRLGAMLRAATEKPALTQSFGIDVPRMLTLTYAGGVALAAFAGVLAAP
                     ILHVNSGMGSNLVIIVFAIVVIGGMGSVLGSIVTALGLGVIEALTKVFYSEASTTVIF
                     VVMALVLLLRPNGLFGREGAA"
     gene            2686..3591
                     /locus_tag="VM406_04610"
     CDS             2686..3591
                     /locus_tag="VM406_04610"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVL75276.1"
                     /translation="MPRHPDLSEEIHNFGEDSVITTVRAEHCEALAARDIAHVAVIDA
                     VEPYTIVRTNLSGAYFQVCLGGEGQTLLDGRWYAHREGAASFAPAHALHALHCVPGKP
                     WKLAWVRFMPSSPYSRSSAMGPRMMRFSGVALEHAILGLYNEMTSGGADSATCAMWVS
                     LIERYAERVIEPWREEPRLMRVWDIVRNDLARDWTLADLAALANTSEEHLRRLCQKCF
                     GRSPGKQLASIRIAHAAHLLATTQDKIDAIAHSVGYVNPFAFSNTFKKLTGFRPSDYR
                     AHPNAVPHGLDSQAGRNPVKVVRKR"
     gene            complement(3541..4245)
                     /locus_tag="VM406_04615"
     CDS             complement(3541..4245)
                     /locus_tag="VM406_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL75277.1"
                     /translation="MHKPLLSIQQLRAWYGPSQALHGVSLDVREGELVTLLGRNGAGK
                     TTLLKAIMGMVGRVEGSIRYRGFELVGDAPHHIARAGIGYCPDDRGIYASLTVRENLL
                     LPPVVSEGGMSEEEIFQLFPNLAERANSPGTKLSGGEQQMLAIARILRTGARLLLLDE
                     PTEGLAPVIVQRIGELLQQLKARGYTILLVEQNVEFAAELADRHYLIETGRVAAEYDA
                     LTLAHDFDRVAARLGV"
     gene            complement(4238..>4398)
                     /locus_tag="VM406_04620"
     CDS             complement(4238..>4398)
                     /locus_tag="VM406_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75278.1"
                     /translation="LSVVSELCDRITVLARGKVIAEGDYASIAADERVIEAYLGRPKR
                     KTDEAAHA"
BASE COUNT          808 a         1520 c         1395 g          675 t
ORIGIN      
        1 gctgctcgcg ccatagaagc cgttgcagtg ctcgctgcgc ttgagcacgt actccacatc
       61 ttccggcgtg gcgatcggcc cgccatgcgc gatgatgatg acatctttgc ggatccgccg
      121 tgccgcctcg ccccattcat cgatcaggcg caccgactcg tcgagcgatt tcgcggtcgt
      181 cgcgccgatg gtgccgccgg tggtcacgcc catgtgcggc acgatcaggt ccgcgccggc
      241 cttggccatc tcgaccgcct cgtcggcgga gaacacgtag ggcgtggtca gcagatcgag
      301 ctcgtgcgcc tggcggatca tgtcgacctc ggacgcgaag cagatcccgg tttcttccag
      361 gctctggcgg aaggtgccgt cgatgatgcc gaccgtcgga aagttctgca cgcccgagaa
      421 gcccatttcc ttcagctgct tcaggaacac cggcatgatg atgaagggat cggtgccgtt
      481 gacgccggcg atcaccggcg tgcgcttgca taccggcaga acctcgcccg ccatctcgac
      541 gacgatttgg ttggcgttgc cgtaggccag caggcccgca gacgacgccc gccccgccat
      601 ccggtagcgc ccggagttgt agatgatgat caggtccgcg ccgcccgctt cctcgcactt
      661 ggccgagatg ccggtcccgg cccctgctcc gacgatcggc tcgcgccggc gcaccatgtc
      721 ctggaagcgc tccagcaaga ccctgcgttc aattctgctc atatgctcga ttccctattt
      781 tgtagtgttg cacttcccga tccccgcact gcgtccgcgg ccccgcgtgc ggcacgccgc
      841 tcggccagct caatcaaggt tccgacgatg ccctttctga acgacagcac gcacaccatg
      901 aacacgatgc cggtgatcag cgtgacccag ccgcccagcg catccaggta gaactgcagg
      961 ccgaccacca gcgctgcgcc gaccgccggg ccgaacaccg tgtgcatccc gcccagaaga
     1021 gccatcagca ccacgtcgcc ggacagatgc cagtgcacgt cggtcagcgc cgccaatccg
     1081 aacaccaggg ccttcagcga gcccgccagg ccggacagcg cggccgacag cacgaacagc
     1141 atcagcttgt agcgggcgac cggatagccg agcgacaccg cgcgcggctc atgctcgcgg
     1201 atcgcgacga tcacgtggcc gaacggcgag tgcacgacgc gatggatgat ccacagcccg
     1261 agcgcggtaa gcgtcatcac gaggtagtac atcgcgacgt cgctgcgcag gtcgatgacg
     1321 ccgagcaggc tgccgcgcgg tatgttctgc atgccgtctt ccgcgccggt gaacggcgcc
     1381 tgcaggaaga tgaagtagac catctgggcc agtgccagcg tgatcatcgc taggtagatg
     1441 ccctggcgcc ggatcgccag cccgcctatg accagcccga gcagagcggc actggccatg
     1501 ccgacgacca ggccaagctc ggtcggcaaa ccccagtgct tggtcgccac gccggtcagg
     1561 tacgcggcgc cgccgaagaa ggccgcgtgg ccgaaggaca tcatgccgcc gaagccgagc
     1621 agcagcccgt aggcgcaggc gaacaaggcc atgcagaaca gcttcatcac gaacaccgga
     1681 tacacgacgg tcggcatcca cggcaccgcc agcagcagga gcaggccgag tatggcggca
     1741 acgcgcaggg tggctcgcat catgccgctc cttcccggcc gaacaggccg ttgggccgca
     1801 acagcagcac cagcgccatc acgacgaaga tcacggtggt cgaggcttcc gaatagaaca
     1861 ccttggtcag cgcttcgatg acgcccagcc caagcgcggt gacgatagac ccgagcaccg
     1921 aacccatgcc gccgatcacc acgatcgcga acacgatgat caccaggttc gagcccatgc
     1981 cggagttcac atgcaggatc ggcgcggcga gcacgccggc gaaggcggcc agcgcgacgc
     2041 cgccggcata ggtgagcgtg agcatgcgcg gcacgtcgat gccgaacgac tgggtaagcg
     2101 cgggcttttc ggttgccgcg cgcagcatcg cgccaagccg cgtgcgctcg atcagccacc
     2161 aggtgcccag acacgcgagc agggcagcta cgatcaccca gatccggtac agcggcatca
     2221 ccatgaagcc catgtcgaac acgccgcgca acgcctccgg taccgggaag ggcttgccgg
     2281 aagtgccgta ggcgacccgg aacccgcctt cgagcgccag caccaggccg aaggtcagca
     2341 gcagcccgta gatcggatcc agtccgtaca ggcggcggat gaagctgcgc tcgaacacgc
     2401 cgccgagcgc gccgacgatc agcggcacga gaatcagcgc gagccagtaa ttgccgccca
     2461 cgtaggtcag cagcatccag gcgccgaagc cgccgagcat gaagaacgcg ccgtgggcga
     2521 aattgacgat cttgagcatg ccgaaaatga cggccaggcc caggctcagt accgcataga
     2581 agcagccgtt gaccagcccg agtacggtct gcccggcgag caccggtagc ggcatgccga
     2641 gaacttcgct cataatcaca gttccttttc tgctcgccga atgccatgcc gcgacatccg
     2701 gacctttccg aggaaatcca caacttcggt gaagacagcg tgatcaccac cgtgcgcgcc
     2761 gagcattgcg aggcgctggc cgcgcgtgac atcgcgcatg tggcggtgat cgacgcggtc
     2821 gagccctaca cgatcgtgcg caccaacctg agcggcgcct acttccaggt ctgcctcggc
     2881 ggcgaaggcc agacgctgct cgacgggcgc tggtacgcgc accgggaagg cgcggccagc
     2941 tttgcgccgg cgcatgcgct gcacgccttg cactgcgtgc ccggcaagcc ctggaagctg
     3001 gcctgggtgc gcttcatgcc gagctcgccc tactcgcgca gcagcgcaat ggggccgcgc
     3061 atgatgcgct tctccggcgt cgcgctcgaa cacgcgatcc tcggcctgta caacgagatg
     3121 acgtccggcg gcgccgacag cgcgacctgc gcgatgtggg tcagcctgat cgaacgctat
     3181 gccgagcgcg tgatcgagcc ctggcgcgag gagccgcgtc tgatgcgggt gtgggacatc
     3241 gtgcgcaacg atcttgcgcg cgactggacg ctggccgacc tcgccgcgct ggccaatacc
     3301 agcgaggagc atctgcgccg cctgtgccag aaatgcttcg ggcgcagtcc cggcaagcag
     3361 ctggccagca tccgcattgc ccacgccgcg cacctgctgg ccacgacgca ggacaagatc
     3421 gacgcgatcg cgcacagcgt cggttatgtg aatccgttcg cgttttccaa cacgttcaag
     3481 aagctgaccg gctttcgtcc gtccgattac cgtgcccacc cgaatgccgt cccgcatggt
     3541 ctagactccc aggcgggccg caacccggtc aaagtcgtgc gcaagcgtta gcgcgtcgta
     3601 ttcggcggcg acacgcccgg tctcgattag gtaatgacgg tccgcgagct cggcggcgaa
     3661 ttccacgttc tgttcgacca gcaggatcgt gtagccgcgc gccttcaact gctgcagcag
     3721 ttcgccgatg cgctgcacga tcaccggcgc gagcccttcg gtcggctcgt cgagcagcag
     3781 caggcgcgcg ccggtgcgca ggatgcgcgc gatcgcgagc atctgctgct ccccgccgga
     3841 gagcttggtg cccggcgaat tcgcacgctc ggccaggttc ggaaacaact ggaatatttc
     3901 ctcctcgctc atgccgccct cggacaccac cggcggcagc agcaggtttt ccctgactgt
     3961 gagggaggcg tagatgccgc ggtcgtccgg gcaatagccg ataccggcac gggcgatgtg
     4021 atgcggcgcg tcgccgacga gctcgaagcc ccgatagcgg atcgagcctt ccacgcggcc
     4081 gaccatgccc atgatggcct tcaacagcgt ggtcttgccg gcgccgttgc ggcccagcag
     4141 cgtgaccagc tcgccttcgc gcacgtcaag actcacgccg tgcagcgcct ggctggggcc
     4201 gtaccacgcg cgcagttgct ggatcgacag gagcggctta tgcatgcgcg gcctcatcgg
     4261 tcttgcgctt cgggcggccg agataggctt cgatcaccct ctcgtcggcg gcaatgctcg
     4321 cgtagtcgcc ttcggcgatc accttgccgc gcgccagcac cgtgatgcgg tcgcacagtt
     4381 ccgacaccac ggaaagat
//
LOCUS       DATLGF010000153         2596 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_133020, whole genome shotgun sequence.
ACCESSION   DATLGF010000153 DATLGF010000000
VERSION     DATLGF010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133020"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /gene="mutL"
                     /locus_tag="VM406_04625"
     CDS             complement(<1..420)
                     /gene="mutL"
                     /locus_tag="VM406_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HVL75279.1"
                     /translation="MTATPATPSLHRPIRALSDQLISQIAAGEVVDRPSAVVKELLEN
                     ALDAGSTQVTVRIEEGGVKRIAITDNGRGIAPEQMPLALARHATSKIASLEDLENVNT
                     LGFRGEALASIASVAQLTLTSRTADAPHAWELNGMPGA"
     gene            complement(485..988)
                     /locus_tag="VM406_04630"
     CDS             complement(485..988)
                     /locus_tag="VM406_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HVL75280.1"
                     /translation="MMQAVANPSAEAADASLAAHEFLAFTLGKEEYGIHILKVQELRG
                     YEEPTRIANAPEFIKGVVNLRGIIVPIVDMRIKFNLGTPTYDQFTVVIILNIAGRVVG
                     MVVDSVSDVITLAPEQVKPAPEMGTALNTDYLIGLGTLDQRMLILVDIDRLMSSAEMG
                     LIDKIAA"
     gene            complement(1225..2526)
                     /locus_tag="VM406_04635"
     CDS             complement(1225..2526)
                     /locus_tag="VM406_04635"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HVL75281.1"
                     /translation="MPRPIEVRPQPLTANKRRVILKSGATLLLSVFAPLPALAAQIVA
                     VRTWPAPDYTRVTLESDVQLSANHFIIKDPERLVVDIEGLALNPTLKELVAKIQPNDP
                     YIEQVRIGQNRPNVVRLVFDLKEEIDPQVFTLAPVGEYRHRLVFDLFPAHAPDPIAAL
                     IRRGGWADETPAEQPPALAEAPRGAQPARAEPQAPARTTRMITIALDPGHGGEDPGAI
                     GRRGTREKDVVLSIARRLKARIDQQPNMRAMLTRTGDYFVPLHVRVQKARQVQADLFV
                     SIHADAWVEPHARGASVFVLSEKGASSTTARWLANKENAADLIGGVNIKSQDRQLAHV
                     LLDLSTTAQINDSLRVGGAVLRELGTINRLHKPTVEQAGFAVLRAPDIPSILVETAFI
                     SNPEEEAKLRNESYQDTVADAILRGIRQYFDKSPLTRTRMM"
BASE COUNT          392 a          803 c          922 g          479 t
ORIGIN      
        1 ggcgccgggc atgccgttca gttcccaggc gtgcggtgcg tcggccgtgc gggaggtgag
       61 tgtcagttgc gcgaccgatg cgatcgaggc cagcgcctcg ccgcgaaagc ctagcgtgtt
      121 gacgttctcc aggtcttcca gcgacgcgat cttcgaggtc gcgtggcgcg ccagcgccag
      181 cggcatctgc tcgggtgcga tgccgcgccc gttgtcggtg atggcgatgc gcttgacccc
      241 gccttcttcg atgcgcaccg tgacctgggt cgagccggcg tcgagtgcgt tttccagcaa
      301 ctccttgacg acggccgacg ggcgatcgac cacctcgccg gcggcgatct gtgaaatgag
      361 ctggtcggaa agcgcccgga tcgggcgatg caaggagggt gtggcgggcg ttgcggtcat
      421 cgccacatta taaggggacg cagggccgcg cccggcggcg gccgggcgtg gacggccggc
      481 aatctcaggc ggcgatcttg tcgatcaggc ccatttcggc gctggacatc aggcggtcga
      541 tgtcgaccag gatcagcatg cgctgatcga gcgtgcccag gccgatcagg taatcggtgt
      601 tgagcgcggt gcccatttcc ggcgccggct tgacctgctc cggcgccagc gtgatcacgt
      661 cggacacgct gtcgaccacc atgccgacga cgcgcccggc gatgttcagg atgatcacga
      721 ccgtgaactg gtcgtaggtt ggcgtgccga ggttgaactt gatgcgcatg tcgacgatcg
      781 gcacgatgat gccgcgcaga ttcacgacgc ccttgatgaa ctccggcgca ttggcgatgc
      841 gagtcggctc ttcgtagccg cgcagttcct gcaccttgag aatatggatg ccgtactctt
      901 ccttgcccag ggtgaaggcg aggaattcgt gggcggcaag gctggcgtcg gccgcttcgg
      961 cgctcgggtt ggctacggct tgcatcatga tgggttcctt tccagtcgcc ggcgcaaaag
     1021 gggcctgggg gcccgtggcg acgtgtgagt gaaggttagc agagtcctgg aaatattgcg
     1081 atagtgcaac atcccggggc acggtgcagg gcggcggcac cgccccgctg tggccgcact
     1141 gcaacaggct gcgcgcaccg ccattgccgt tcgatcaagc cgctgcgagt gcccgccatc
     1201 cgcgtcccgt cgaggcgcgg cgcctcacat catccgggtt cgcgtcagcg ggctcttgtc
     1261 gaagtactgt cgaattcctc gcaggatcgc gtcggccacc gtgtcctggt agctctcatt
     1321 gcgcagcttg gcttcttcct cggggttgga gatgaaggcg gtttcgacca ggatgctggg
     1381 aatgtcgggc gcgcgcagca ctgcgaagcc ggcctgctcg accgttggct tgtgcagccg
     1441 gttgatcgtg ccgagctcgc gcagaacggc gccgcccacg cgcaggctgt cgttgatctg
     1501 ggcggtggtg gacaggtcga gcagcacatg ggcgagctgg cggtcctgcg acttgatgtt
     1561 gacgccgccg atcaggtcgg ccgcgttttc cttgttcgcc agccagcgcg cggtggtcga
     1621 cgacgcgccc ttctcggaca gcacgaacac cgacgcgccg cgcgcatgcg gttccaccca
     1681 ggcatcggcg tggatcgaca cgaacaggtc ggcctgaacc tggcgcgcct tctgcacgcg
     1741 tacgtgcagc ggcacgaaat agtcgccggt gcgcgtgagc atcgcgcgca tgttcggctg
     1801 ctggtcgatc ctggccttga gccggcgggc gatggacagc accacgtcct tttcgcgggt
     1861 gccgcggcgg ccgatggcgc cgggatcctc gccgccatgt ccgggatcga gcgcgatggt
     1921 gatcattcgc gtggtacgcg cgggcgcctg cggctccgcg cgcgccggct gcgcgccgcg
     1981 gggggcttcg gccagcgcgg gaggctgctc ggccggcgtc tcatcggccc agccgccgcg
     2041 acggatcagt gcggcgatcg gatcgggcgc atgcgcgggg aacaggtcga acaccaggcg
     2101 gtggcgatat tcgcccacgg gagccagcgt gaatacctgc gggtcgattt cttccttcag
     2161 atcgaacacc aggcgcacca cgttcggccg gttctggccg atgcgcacct gctcgatgta
     2221 gggatcgttg ggctggatct tcgcgaccag ttccttgagc gtcgggttga gcgccagccc
     2281 ttcgatgtcc accaccaggc gctccgggtc cttgatgatg aaatggttgg cgctgagctg
     2341 cacgtcgctt tccagcgtga cgcgcgtgta gtccggggcc ggccaggtgc gcacggcgac
     2401 gatctgcgcg gcgagggccg gaagcggggc gaagacggag agcagcagcg tggcaccgct
     2461 tttcaggatg acgcgacgct tgtttgcagt cagaggttgg gggcgaactt caatcgggcg
     2521 aggcattgac ttccctgctc ggacaatgcc tgcaattcta acccacgagc aacatcgatg
     2581 atgctcagat gcacat
//
LOCUS       DATLGF010000154         3961 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_162883, whole genome shotgun sequence.
ACCESSION   DATLGF010000154 DATLGF010000000
VERSION     DATLGF010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162883"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VM406_04640"
     CDS             complement(<1..479)
                     /locus_tag="VM406_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HVL75282.1"
                     /translation="MELTGKRIVLGLTGGIACYKAAELTRMLTKAGASVQVVMTSAAA
                     QFITPVTMQALSGKTVYTDQWDARIPNNMPHIDLTRDADAILVAPCSADFLFKLAHGA
                     CDDLLSTLCLARPATLPLLVAPAMNVEMWQNPATQRNVAQLRADGVAILGPEAGDQA"
     gene            complement(520..978)
                     /gene="lspA"
                     /locus_tag="VM406_04645"
     CDS             complement(520..978)
                     /gene="lspA"
                     /locus_tag="VM406_04645"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HVL75283.1"
                     /translation="MMRWLVIAALIVLVDQITKVSITQFFSYGESRPVTGFFNLVLAH
                     NPGAAFSFLAGAGGWQRWFFTGIAVVAVVTILFLLKRHSGQRMFCWALALILGGAIGN
                     LIDRTVYGYVIDFLDFHVAGWHWPAFNVADMAIVGGAGLFILDELRRVGK"
     gene            complement(1046..3925)
                     /gene="ileS"
                     /locus_tag="VM406_04650"
     CDS             complement(1046..3925)
                     /gene="ileS"
                     /locus_tag="VM406_04650"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HVL75284.1"
                     /translation="MSEQKNKYPVNLTETTFPMRGDLAKREPKWVQEWQAKEIYKRIR
                     KASAGRPKWILHDGPPYANGDIHIGHAVNKILKDTIVKSRNMAGYDAQYVPGWDCHGM
                     PIEIQIEKQFGKNLPTAEVLAKSRAYAAEQIERQKKDFMRLGVLGDWDNPYKTMNFRN
                     EADEIRALGLILEKGYVYRGLKPVNWCFDCGSALAEAEVEYQDKRDPEIYVGFPAEDR
                     DKLAQAFGLATLPAEAVYAVIWTTTPWTIPSNQALNVHPEFSYQLLRLEGGKHHGRHV
                     IIAAERVAPFLKEIGEKQNGDTAEAAATVLGETTGTKLAHVRFKHPFAHLDPAYNRFS
                     PVYLGDYVTLDAGTGIVHSAPAYGVEDFISCKTHGMRDDEILNPVMGDGRFASWLPFF
                     GGLNIWDASKPICAKLDEVGALFGFGIKPHSYMHCWRHKTPIIYRATSQWFAGMDTVP
                     KDNGPTLRETALAGIEATAFFPAWGKARLHGMIANRPDWTLSRQRQWGVPMAFFVHKE
                     TGELHPRTLELLEEVARRVEQNGIEAWHALDPKELLGEDAALYEKNRDTLDVWFDSGT
                     THHTVLRGSHADESQFPADLYLEGSDQHRGWFHSSLLTSSMINRQPPYKALLTHGFVV
                     DGEGKKMSKSKGNVVAPQKVSDTLGAEILRLWVASTDYSGELSISDEILKRVVEAYRR
                     IRNTLRFLLANTSDFDPGKDAVPVAELFEIDRYAIARMAELQGEILAHFEVFEFHPVV
                     GKLQMYCSEDLGGFYLDILKDRLYTCGADSRARRSAQNALWHITHALLRVMAPMLSFT
                     AEEAWAAFAGKETYEASSETIFTQTYYVLPEIADGADLLARFGALRELRAQVTKQLEE
                     VRMAGGIGSSLQAEIEIKASGAKYAMLKSFGDDLKFVFITSSATVSEVANDAEEAVIV
                     TPSAHQKCERCWHYREDVGVDAAHPGICGRCVSNLFGAGEARQAA"
BASE COUNT          670 a         1227 c         1299 g          765 t
ORIGIN      
        1 caggcctgat cgccggcttc cggcccgagg atcgcaacgc cgtccgcgcg cagttgcgcg
       61 acgttgcgct gggtcgcagg gttctgccac atctccacgt tcatcgccgg cgcaaccagc
      121 aggggcaggg tggccggacg cgccaggcac agcgtcgaga gcaggtcgtc gcaggccccg
      181 tgggcgagct tgaacaggaa atcggccgag cacggcgcga ccaggatcgc atcggcgtcg
      241 cgcgtcaggt cgatgtgcgg catgttgttc ggaatgcgcg catcccactg atcggtgtag
      301 acggtcttgc ccgacagcgc ctgcatcgtg actggcgtga tgaactgggc ggcggcactg
      361 gtcatcacga cctggaccga tgcaccggcc ttggtcagca tgcgcgtcag ttccgccgcc
      421 ttgtagcagg cgatgccgcc ggtgaggccg agtacgatgc gtttgccagt gagttccatg
      481 tgtgttcccg gtttgtgtca gcagcgcgct gcgctagttc tactttccca cccgcctcaa
      541 ttcatccagg atgaacagtc ccgcgccgcc aacgatcgcc atatccgcaa cattgaacgc
      601 aggccaatgc cagccggcga cgtggaaatc gaggaagtcg atcacgtagc catagacggt
      661 gcgatcgatc aggttgccga tcgcgccgcc gaggatcagg gccagcgccc agcagaacat
      721 ccgctggccc gagtgacgct tgagcaggaa gaggatggtc accacggcca cgaccgcgat
      781 accggtgaag aaccagcgct gccagccacc cgcgccggca agaaagctga atgccgcccc
      841 gggattgtgc gccagcacca ggttgaaaaa gccggtgacc gggcgcgact cgccatagct
      901 gaagaactgg gtgatgctca ccttggtgat ctggtcgacc agcacgatca gcgctgcgat
      961 gactagccag cgcatcatgc ccggctcgtt ggcggaaaag gttttcttgt atgccattgt
     1021 ggttccggtt ggtcctgttt accgctcagg cggcttgccg ggcttcgccc gcgccgaaca
     1081 ggttcgagac gcagcgcccg cagatgcccg gatgggctgc atcgacgccg acgtcttcgc
     1141 ggtaatgcca gcagcgctcg cacttctgat gggccgacgg cgtgacgatc accgcttcct
     1201 ccgcatcgtt cgcgacttcg ctcacggtgg cggacgaggt gatgaacacg aacttcaggt
     1261 cgtcgccgaa gctcttgagc atcgcatatt tcgcgccgct ggccttgatc tcgatctccg
     1321 cctgcagcga ggagccgatg ccgccggcca tgcgcacttc ctcgagctgc ttggtaacct
     1381 gcgcgcgcag ttctcgcagc gcgccgaacc tggccagcag gtcggcgcca tcggcgatct
     1441 ccggcagcac gtagtaggtc tgcgtgaaga tggtttcgct gctcgcctca tacgtttcct
     1501 tgccggcgaa cgcggcccac gcctcttctg cggtgaacga gagcatcggc gccatcacgc
     1561 gcagcagcgc atgggtgatg tgccacagcg cgttctgggc cgaacggcgt gccctggaat
     1621 cggcgccgca ggtgtacagg cggtccttca ggatgtcgag gtagaagccg ccgaggtctt
     1681 ccgagcagta catctgcagc ttgccgacga ccgggtggaa ctcgaacact tcgaagtgcg
     1741 caaggatctc gccctggagt tcggccatgc gcgcgattgc atagcggtcg atctcgaaca
     1801 gttcggcaac cggcaccgca tccttgccgg gatcgaaatc cgaggtgttg gcgagcagga
     1861 agcgcagcgt gttgcggatg cggcggtagg cctcgaccac gcgcttgagg atctcgtcgg
     1921 agatcgacag ttcgccggag tagtcggtgg acgcgaccca gagacgcagg atttcggcgc
     1981 ccagcgtgtc cgacaccttc tgcggcgcga ccacgttgcc cttggacttc gacatcttct
     2041 tgccttcgcc gtccaccacg aagccatgcg tgagcagggc cttgtacggc ggctggcgat
     2101 tgatcatcga ggaggtcagc agcgaggaat gaaaccagcc gcggtgctgg tccgagcctt
     2161 cgaggtagag gtcggccggg aactgagatt cgtccgcgtg cgagccgcgc agcaccgtat
     2221 ggtgggtggt gccggaatcg aaccagacgt cgagcgtgtc acggtttttt tcgtacagcg
     2281 cggcatcttc gccgagcagt tccttcggat cgagcgcatg ccaggcttcg atgccgttct
     2341 gctcgacgcg tctggcaact tcctcgagca gctcgagcgt gcgcggatgc agctcgcccg
     2401 tttccttgtg cacgaagaat gccatcggca cgccccactg gcgctggcgc gaaagcgtcc
     2461 agtccgggcg gtttgcaatc atgccgtgca gacgcgcctt gccccaggcg gggaagaagg
     2521 cggttgcctc gatgccggcc agcgcggttt cgcgcagcgt cggcccgtta tccttgggca
     2581 ccgtgtccat gccggcgaac cattgcgagg tcgcacgata gatgatcggc gtcttgtggc
     2641 gccagcagtg catgtagctg tgcggcttga tgccaaagcc gaacagcgcg ccaacttcgt
     2701 cgagcttcgc gcagatgggc ttggacgcat cccagatgtt caggccaccg aagaacggca
     2761 gccaggaagc gaacctgccg tcgcccatca ccgggttcag gatttcgtcg tcgcgcatgc
     2821 catgggtctt gcacgagatg aagtcttcca cgccgtaggc cggcgcggag tggacgatgc
     2881 cggtgccggc gtcgagggtc acgtagtcgc cgaggtagac cggagaaaag cggttgtacg
     2941 cgggatcgag atgggcgaac ggatgcttga agcgcacgtg cgcgagcttc gtgccggtgg
     3001 tttcgccgag gacggtcgcg gccgcttcgg cagtatcacc gttctgcttc tccccgatct
     3061 ccttcaggaa cggcgcaacg cgttcggctg cgatgatcac atgcctgccg tggtgcttgc
     3121 cgccctccag ccgcagcaac tggtagctga actcgggatg gacgttgagt gcctggttgg
     3181 acggaatggt ccacggcgta gtggtccaga tcaccgcgta gaccgcttcg gccggcagcg
     3241 tggccaggcc gaacgcctgc gcgagcttgt cgcggtcctc ggccgggaag ccgacgtaaa
     3301 tttccgggtc gcgcttgtcc tgatattcga cctccgcctc ggccagcgcc gagccgcagt
     3361 cgaagcacca gttcaccggc ttcaggccgc ggtagacata gcccttctcg aggatgaggc
     3421 cgagcgcgcg gatctcgtcg gcctcattgc ggaagttcat cgtcttgtac ggattgtccc
     3481 agtcgccaag cacgcccagg cgcatgaaat ccttcttctg gcgctcgatc tgctcggcgg
     3541 cgtaggcgcg cgacttggcc agcacttcgg cagtcggcag gttcttgccg aactgcttct
     3601 cgatctggat ctcaatcggc atgccgtggc aatcccagcc gggcacgtac tgcgcgtcgt
     3661 agccggccat gttgcgcgac ttgacgatgg tgtccttcag gatcttgttg accgcgtggc
     3721 cgatgtgaat gtcgccgttc gcgtacggcg ggccgtcgtg caggatccac ttcgggcggc
     3781 cggcggatgc cttgcggatg cgcttgtaga tctctttggc ctgccactcc tgcacccatt
     3841 tcggttcgcg cttggcaaga tcgccgcgca tcggaaaggt ggtttcggtc aggttgaccg
     3901 ggtacttgtt tttttgctcg gacatgatgg atttccgtat cggtgttcgg tggatcaaac
     3961 g
//
LOCUS       DATLGF010000155        11363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_22878,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000155 DATLGF010000000
VERSION     DATLGF010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 11363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11363
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22878"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1020)
                     /locus_tag="VM406_04655"
     CDS             complement(70..1020)
                     /locus_tag="VM406_04655"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008110854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HVL75285.1"
                     /translation="MALENLMVFTGNANPELAAGVAKQLRIPLGKAVVSKFSDGEVMV
                     EINENVRGKDVFVLQSTCAPTNDNLMEIMLMVDALKRASAGRITAAIPYFGYARQDRR
                     PRSARVAISAKVVANMLQQAGVERVLIMDLHADQIQGFFDIPVDNIYASPILLSDLIK
                     KNYDDLLVVSPDVGGVVRARALAKRLDCDLAIIDKRRPKANVSEVMHIIGEVEGRNCV
                     IMDDMVDTAGTLTKAAEVLKERGAKKVVAYCTHPVLSGPAIERIATSPLDELVVTDTI
                     PLTADARTCSKIRQLTCADLLAETFKRITTGDSVSSLFAD"
     gene            complement(1147..1223)
                     /locus_tag="VM406_04660"
     tRNA            complement(1147..1223)
                     /locus_tag="VM406_04660"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1187..1189),aa:Gln,seq:ttg)
     gene            complement(1274..2143)
                     /gene="ispE"
                     /locus_tag="VM406_04665"
     CDS             complement(1274..2143)
                     /gene="ispE"
                     /locus_tag="VM406_04665"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HVL75286.1"
                     /translation="MATDVLNNCPAPAKLNLFLHVTGRRADGYHELQTVFQLLDRGDV
                     LHFRLREDGEIRRVSDLPGVPAENDLVVRAARLLQSHAGCAQGVDIAVEKHLPMGGGL
                     GGGSSDAATTLLALNHLWRTGLSRTELMQLGLRLGADVPFFVFGQNAFAEGVGEALVA
                     VATPDCWYVVVEPGVETPTAQIFSDPHLTRDTKAVRISDFSGASVGFGKNDLQAVATR
                     LFAPISEALGWLGQFGQARMTGSGACVFCAFAKEQDADAVLEAARVTGKPAWKVWKAK
                     AIERHPLSHLLQS"
     gene            complement(2125..2736)
                     /locus_tag="VM406_04670"
     CDS             complement(2125..2736)
                     /locus_tag="VM406_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018057790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HVL75287.1"
                     /translation="MTGSAIRIALLALPLLLTACAPLQPAAPAAPAAIRAYQQSIELG
                     GRLSVRYETLHGEEALHGGFQWSQAGPATSVVLLSPLGQAMARIDVTPATATLLQAGQ
                     PARSAPDPDTLTATTLGWPLPVAGLRDWLQGFAIDEQGRQFIASPQNTSVNTRDGWLV
                     LYPAWVDDGAGTPRPRRIDLSRRTAQAGEVAIRIVIDTWQPTS"
     gene            complement(2733..4442)
                     /locus_tag="VM406_04675"
     CDS             complement(2733..4442)
                     /locus_tag="VM406_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVL75288.1"
                     /translation="MRRVVAQAPAGAAPASQHAADAAEVPAPQEPLPHMALTRELMFR
                     ILGAEIAFQRGEWQSAYITLLGLAQQTRDPRLARRAAEFAHLARQQAEVLTAVRLWRE
                     LAPHSDEANQYFLGFVIMGDHLEEARPVLEQRLKDAPAQMRGALAFQFQQHLARARDK
                     AAAFKLLDQVLAPYVAIPEVRLAIAQAAVTNGDLDRARNEARQALAAKPDSELAILSL
                     AQATPDKADATSLLEGFLKRQPQSREVRLARARLLVEQQQYQTARAEFDRLLRDNPQD
                     LTALYALGVLGVQTGDLAGAERHLHAYLEVLEQNPDAERDPSQAVLLLAQIAEERKDT
                     DAMLKWLSRIEGGEVYVSAQIRRAQLLAKGGDVEQARSLLRQVNPASERDDAMLIMAE
                     GQLLRDAGRLDEALKVYETGLARLPDNTDLLYDHAMLAEKRGQWDLMETSLRKIIEIS
                     PQHQHAYNALGYSLADRNIRLQEAFELISKALALAPDDAFTIDSMGWVQYRLGRLDEA
                     EKLLRRAYSLRQDAEIATHLGEVLWVRGQREAALQLWREAQAKDPQNDTLKSTLARLN
                     AEL"
     gene            4740..5561
                     /gene="mutM"
                     /locus_tag="VM406_04680"
     CDS             4740..5561
                     /gene="mutM"
                     /locus_tag="VM406_04680"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HVL75289.1"
                     /translation="MPELPEVEVTRLGVAPHIEGRRVTGVTLRHTGLRWPFPVQLAER
                     LCGRTVRATARRGKYLLIRFDHGDLIVHLGMSGHLRILPVGTPAQKHDHFDLELGEHL
                     LRMNDPRRFGAILWHGREEGDIEEHLLLRGLGVEPLGEGFDGALLYRATRGRSAPIKQ
                     VLLAGDIVVGVGNIYACESLFKAGIHPKTAAGRIGLARYKKLAAAIRETLAAAIAQGG
                     STLRDFVAVNGQSGYFQQSYFVYSRAGEACRICRGNVRLLRQGQRSTFYCPNCQK"
     gene            5612..7549
                     /locus_tag="VM406_04685"
     CDS             5612..7549
                     /locus_tag="VM406_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HVL75290.1"
                     /translation="MSNLVQNFQQYSGWRSAVMAALERYRACVAAADLADAASDQRFA
                     RMLARLNDDKLTIAFVAEFSRGKSELINAIFFADYGQRILPSSAGRTTMCPTELLYDE
                     TFPPSIRLLPIETRAEAQSTSDYKEQSHVWTVLPLDIQSGEGMLDAFRQVSLTKRVPV
                     DEARRYGLYHDDDPDAALSVGEDGLVEISRWRHAIVNFPHPLLKQGLVIIDTPGLNAI
                     GTEPELTLNLIPNAHAVLFILAADTGVTRSDIEVWRDHIGGGPARMVVLNKIDSMWDE
                     LRGAEEVERQIAGQVAAVAQTLSLSERQVYPVSAQKGLVGKVNQDAALLAKSRLPQLE
                     SALSGELIPSKQRIVREQLHAEIGELTSAKQALLAARTRNVVEQLVELKSLRGKNQNI
                     IEHMMKRVDLEKKEFDASLLKLQGTRAVFTRLSTEVFTSLGMDVLKEDIRRTREAMDR
                     SKFSAGLRSAVREFFEQIRKNLTASSQKTDEITEMMTIMYRRFSSEHGLALSTPMPFT
                     LEKYLREVEIIEGVYHKQFGTATLLTTPQVVLMRKFFDSIASRVKQSFLQANRDVEAW
                     LKVVMAPLEAQVWEHKAQLKKRRQSIERIHMATDSLEEKLEALEAMQAELEEQKKTLL
                     QIEGELNAAIASQLTSLRAAA"
     gene            7565..8692
                     /gene="mutY"
                     /locus_tag="VM406_04690"
     CDS             7565..8692
                     /gene="mutY"
                     /locus_tag="VM406_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HVL75291.1"
                     /translation="MKRVVDRAPHAAEPGISLADPEFSRSVIEWQRSHGRHALPWQNT
                     RDAYRIWLSEIMLQQTQVSAVIPYYLRFLERFPDVAALAAGAQEDVMAHWSGLGYYSR
                     ARNLHRCAQRIVAEYGGSFPRDPAALAALPGIGRSTAAAIAAFAYGERAAILDGNVKR
                     VFCRVFGIEGFPGAKPIENALWERAQALLPEQGVEAYTQGLMDLGATVCTRGKPSCAR
                     CPLSSRCDAYASNRTHLLPTARPAKEVPRRSVAMLVIEAGEHVLLEQRPDSGIWGGLL
                     SLPEIPAETAEEGRVTQAAARFGHVEAVERLQPFTHVFTHFRLDVLPFRVRLTQPVTF
                     AGEVRYVWSAHKGVADAALPAPVKKLLLSLYSRTGFQAQLV"
     gene            complement(8689..9810)
                     /locus_tag="VM406_04695"
     CDS             complement(8689..9810)
                     /locus_tag="VM406_04695"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVL75292.1"
                     /translation="MSANRMFLDDAREATRPESLDRRKPQSGSTLPQAAPVALTGALY
                     VASLFVFFRHGFIDRVTLASAAGAAALIIGMFQLVFHKGWNRRLDDPALMLPQLFASL
                     VLMLGVAWMERATQVALVPFALIAFSFGLFRLSAASLTLLATAFLLAYLGMIVVRARA
                     EGFDFGFHSDLVQWCVLALTLPGMILVARQIHNLRQTLRSTRSELQHFEEKAARDELT
                     GLFNRRQLQTELNQAKLRADRHGQTFSLCLIDVDHFKDINDSHGHLAGDIILREFSRM
                     ARESVREADVLGRYGGDEFMQILHDTDVKGAVMHAERLRVYAHFLDFGKVLEQGKISL
                     SIGVAQYRRGERIDDLISRADSALYQAKQLGRNRVEWVD"
     gene            complement(10015..10641)
                     /locus_tag="VM406_04700"
     CDS             complement(10015..10641)
                     /locus_tag="VM406_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HVL75293.1"
                     /translation="MPNDLGWLPLFPLKTVLFPDGILPLRIFETRYIDMVRDCMKTGA
                     PFGVALIRNGQEVGAAAEPEPVGCLARITDWDMQELGVMHLRVQGGERFRVLETRVRA
                     DQRLEARVEAIEPDPEIPVSDIHVRCAKTLKIVIDDIESRARAAQDGEFVSPFAYPIR
                     LDSAAWVANRWCEILPIPLKARQKLLELEDPGARLSLVYQYLQQHKII"
     gene            complement(10646..>11363)
                     /gene="rapZ"
                     /locus_tag="VM406_04705"
     CDS             complement(10646..>11363)
                     /gene="rapZ"
                     /locus_tag="VM406_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HVL75294.1"
                     /translation="ALAVAVDVRSADSLADLPGDIRTLKEQGHDVKVLFLTAKTESLI
                     TRFSETRRSHPLSHRLRSAQPNGGQPTLTECILQEREMLSSIEGLGHVIDTSGLSANK
                     LRSWIKDLVGNERSPLTLLFESFAFKFGVPLDADLVFDVRVLPNPYYDTNLRPFTGRD
                     TPVVAFLDAQEQVGELLADIRGFVEKWLPAFRQDNRSYLTVAIGCTGGQHRSVYMVEK
                     LARHFSGGAERVLLRHRELN"
BASE COUNT         1976 a         3614 c         3791 g         1982 t
ORIGIN      
        1 cggtttgtcc cccgcgacca ggggacatga aaaacccggc cccctgttcg aggggccggg
       61 cgtcaaaatt taatcggcaa acagcgagct gaccgaatcg ccggtggtga tgcgcttgaa
      121 ggtctcggca agcaggtccg cgcaggtgag ctggcggatc ttgctgcagg ttcttgcgtc
      181 ggcggtcagc ggaatggtgt cggtaacgac cagttcgtcc agcggcgacg tggcgatgcg
      241 ctcgatcgcc gggcccgaca gcaccgggtg cgtacagtag gcgaccacct tcttcgcgcc
      301 gcgttccttc agcacctcgg cggccttggt cagcgtgccg gcggtgtcga ccatgtcgtc
      361 catgatcacg cagttgcggc cctcgacttc accgatgatg tgcatgactt ccgacacgtt
      421 cgccttcgga cgacgcttgt cgatgatggc caggtcgcag tccaggcgct tggccagcgc
      481 acgggcgcgc accacgccgc cgacatcagg cgagaccacc agcaaatcgt cgtaattctt
      541 cttgatcagg tccgacagca ggatcggcga cgcatagata ttgtcgacgg ggatatcgaa
      601 gaagccctgg atctggtccg cgtgcaggtc catgatcagc acacgctcga caccggcctg
      661 ctgcagcatg ttcgcaacca ccttggccga gattgccacg cgcgccgaac gcgggcgccg
      721 gtcctggcgg gcgtagccga agtaggggat cgcggcggtg atgcggccgg cggaggcgcg
      781 cttcaacgcg tcgaccatca acatgatttc catcaggttg tcgttggtcg gcgcgcaggt
      841 cgattgcaga acgaatacat ccttgccgcg gacgttttcg ttgatctcca ccatgacttc
      901 cccatcggag aacttggaga cgacggcctt gccgagcgga atgcgaagct gcttggcgac
      961 gccggcagcc agttcggggt tggcattgcc ggtaaagacc atcaggtttt caagcgccat
     1021 gggacaccta gtgcaatcgt ttttctgagg ggggtagaag ggagaaaagc cgccaaaact
     1081 tgactggttg cccatcgggc gtcgtctcgt gttggcggct tttctcgggt ttcttctaga
     1141 acttaatggc aggggaggaa ggactcgaac cctcgcatgc cggaatcaaa atccggtgcc
     1201 ttaaccaact tggcgactcc cctacacaac cttgctgtct gccgccggtc actacgacgt
     1261 cagacaaatt cctttacgac tgcaacaagt gcgagagcgg gtggcgctcg atcgcctttg
     1321 ctttccagac cttccaggcc ggcttgccag tgacgcgcgc cgcttccagc actgcgtcag
     1381 catcctgctc cttggcgaat gcgcagaaca cgcatgcgcc ggagccggtc atccttgcct
     1441 gtccgaactg ccccagccat cccagtgctt cggagatcgg tgcaaagagc cgtgtggcaa
     1501 ccgcttgcag atcgtttttt ccgaatccga cagaagctcc ggaaaagtcc gatattctga
     1561 cggctttcgt atcccttgtc aaatgcggat cggaaaaaat ttgcgcggtc ggcgtttcaa
     1621 cccccggctc gactacgaca taccagcagt ccggggtggc gaccgcgacc agcgcttcgc
     1681 cgaccccctc ggcgaacgcg ttctgtccga acacgaagaa cggcacgtcc gccccgagcc
     1741 gcaggcccaa ttgcatcaac tcggtgcggg agagcccggt ccgccacagg tggttcagcg
     1801 ccagcagcgt ggtggcggca tcggaggagc caccgcccag gccgccgccc atcggcaggt
     1861 gcttttcgac ggcaatgtct acaccttgcg cgcagccggc atgcgactgc aggaggcgcg
     1921 cggcgcgcac gaccaggtcg ttctcggccg gcacgccggg cagatcgctc acccgccgga
     1981 tctcaccgtc ctcgcgcagc cggaagtgca gcacatcgcc acggtcgagc agctggaaca
     2041 ccgtctgcag ttcgtggtag ccgtccgcgc ggcggccggt gacatgcagg aacaggttga
     2101 gcttggccgg cgcggggcag ttgttcagga cgtcggttgc catgtgtcga tcacgatgcg
     2161 gatggccacc tcgccggctt gcgcggtgcg gcgggaaaga tcgatgcggc gcggacgcgg
     2221 cgttccggcg ccgtcgtcca cccaggccgg gtagagcacg agccagccgt cgcgggtatt
     2281 gacggacgtg ttttgcggac tggcgatgaa ctggcggccc tgctcatcga tcgcgaagcc
     2341 ctgcagccag tcgcgcaggc ccgccacagg taacggccag ccgagcgtag tggcggtcag
     2401 ggtatccggg tccggcgcgc tgcgcgccgg ctgtcctgcc tgcagcaggg tggccgtcgc
     2461 gggcgtgacg tcgatgcgcg ccatcgcctg accgagcggg gagagcagga ccaccgacgt
     2521 ggcaggtccg gcctggctcc attggaagcc gccgtgcagc gcttcttcgc cgtgcagggt
     2581 ttcgtagcgc accgacagcc ggcctcccag ttcgatgctc tgctgatacg cgcgaatggc
     2641 cgcaggagcg gccggcgcgg ccggctgcag cggcgcgcag gccgtgagca gcagcggcag
     2701 cgcgaggagc gcgatccgga ttgccgatcc cgtcatagct cagcgttgag ccgtgccagc
     2761 gtgctcttga gcgtgtcgtt ctgcgggtcc ttcgcctggg cttcgcgcca cagctggagc
     2821 gccgcctcgc gctggccgcg cacccacagc acttcaccaa gatgggtggc gatctcggcg
     2881 tcctggcgca gagagtatgc acggcgcagc agcttttccg cttcgtcgag gcggcccagc
     2941 cggtactgca cccaccccat gctgtcgatg gtgaaggcgt cgtcaggcgc cagcgcgagc
     3001 gccttgctga tcagctcgaa cgcttcctgc aggcggatgt tgcggtcggc cagcgaatag
     3061 ccgagcgcgt tgtacgcgtg ctggtgctgc ggggatatct cgatgatctt gcgcagcgag
     3121 gtctccatca gatcccattg gccgcgcttt tccgccagca tcgcatggtc gtacagcagg
     3181 tcggtgttgt ccggcaggcg cgcgaggcca gtctcgtaca ccttgagcgc ctcgtcgaga
     3241 cggccggcgt cgcgcaacag ctggccctcg gccatgatca gcatcgcatc gtcgcgctcg
     3301 ctggccggat tcacctgccg cagcagcgag cgcgcctgtt cgacgtcgcc gccctttgcc
     3361 agcagctggg ctcggcggat ctgcgcactg acgtagactt cgccgccttc gatgcgcgag
     3421 agccacttca gcattgcgtc ggtgtccttg cgctcctcgg cgatctgcgc aagcagcagc
     3481 accgcctgcg acggatcgcg ttcggcatcc ggattctgct ccagcacctc aaggtaggca
     3541 tgcagatgac gctcggcgcc ggcgaggtcg ccggtctgca cacccagcac gccgagcgca
     3601 tagagggcgg tgaggtcctg cggattgtca cgcaggagcc gatcgaactc ggcgcgtgca
     3661 gtctggtact gctgctgctc gaccaggagc cgcgcccggg caaggcgcac ttcgcgcgac
     3721 tgcggctgcc gcttcaggaa gccctccagc agcgacgtcg catcggcctt gtccggcgtg
     3781 gcctgggcca gcgacaggat cgccagctcg gaatcgggtt tggccgccag cgcctgcctg
     3841 gcttcgttgc gcgcccggtc gaggtcgcca ttggtcacgg ccgcctgggc gatggccagg
     3901 cgcacctcgg gaatcgccac atacggcgcc agcacctgat cgaggagctt gaaggccgct
     3961 gccttgtcgc gcgcgcgcgc caaatgctgc tggaactgga aggccagcgc accgcgcatt
     4021 tgcgccggcg catccttgag ccgctgttcg aggacagggc gggcttcttc gagatggtcg
     4081 cccatgatca cgaagccgag aaaatactgg ttggcttcgt cggaatgcgg ggcgagttcg
     4141 cgccacaggc gcactgcggt caagacctcg gcctgctggc gggccaggtg ggcgaattca
     4201 gcggctcggc gcgccaggcg cgggtcccgg gtctgctggg caaggccgag cagggtgatg
     4261 taagccgatt gccactcgcc gcgctggaat gcgatctcgg cgccgaggat gcgaaacatc
     4321 agttcgcgcg taagcgccat gtgcggcagg ggctcctgcg gcgccggcac ctcggcggca
     4381 tccgccgcgt gctgcgacgc cggcgccgca ccggcaggtg cctgggcgac gacgcgccgc
     4441 acgggtacag gctggctgcc agcttcgggc gctacggcat tgcttgccgc catcggcgcg
     4501 tgagttgcgc atgcggagac ggcgagcgag agagttacaa tgagcagcgg ttttttcaat
     4561 gtatggttcc ggcgggtcga gcaggtctgc cggcatgcat ggtggacaag tgctgcgtgg
     4621 cacttgtccc gcggtgccag cctgggcaat tctacgccct tcgcgcgccc ggtgcgcgtc
     4681 cgcaaccccc tgtttgctca aggattctgc ccgcattgcg cggctgaaaa taaggtctga
     4741 tgcctgaatt gccagaagtg gaagtgacgc gcctgggcgt cgccccgcac atcgagggcc
     4801 gccgcgtaac cggcgtgacg ctgcgccata ccggcttgcg ctggccgttt cccgtgcagc
     4861 ttgccgagcg gctgtgcggc cgcaccgtgc gcgcgaccgc gcggcgcgga aagtacctgc
     4921 tgatccgctt cgaccatggg gacttgatcg tccacctcgg catgtccggc cacctgcgca
     4981 tacttcccgt ggggacgccc gcgcagaagc atgatcattt cgacctcgaa ctcggcgaac
     5041 atctgctgcg catgaacgat ccacgccggt tcggcgcgat tctctggcac ggtcgcgagg
     5101 aaggcgacat cgaagagcat ctgctgctgc gcggactagg cgtggagccg ctgggcgagg
     5161 gattcgacgg tgccttgctc taccgggcca cacgcgggcg cagcgcgccg atcaagcagg
     5221 tgttgctggc tggcgacatc gtggtcggcg tcggcaacat ctacgcctgc gagagcctgt
     5281 tcaaggccgg catccatcca aagaccgcgg ccgggcgcat cgggctggcg cgctacaaga
     5341 aacttgccgc ggcgatacgc gagacgctgg ccgccgccat cgcgcagggc ggcagcacgc
     5401 tgcgcgattt cgtcgccgtc aatgggcagt ccggctactt ccagcaaagc tacttcgtct
     5461 acagccgcgc cggtgaagca tgccgcatct gtcgcggcaa cgtgcgcctg ctgcgccagg
     5521 ggcaacgttc gaccttctac tgcccaaatt gtcaaaagtg agggccggtg ctagattcga
     5581 gccttgttac gcgtgagaaa ccggggatga tgtgagcaat ctggtccaga acttccagca
     5641 atacagtgga tggcgcagcg cggtgatggc cgcgctcgag cgttatcggg cctgcgtcgc
     5701 cgctgccgat ctcgctgacg ctgccagcga ccagcgcttc gcgcgcatgc tcgcgcgcct
     5761 gaacgatgac aagctcacca tcgccttcgt cgccgagttc tcgcgcggca aatccgagct
     5821 gatcaacgcg atcttcttcg ccgactacgg ccagcgcatt ctgccgtcct cggccgggcg
     5881 cacgacgatg tgcccgaccg agttgctgta cgacgaaacc ttcccgcctt cgatccgcct
     5941 gttgccgata gaaacacgcg ccgaggcaca gtccaccagc gactacaagg aacagagcca
     6001 tgtctggacc gtgctgccgc tcgacatcca gtcgggcgaa ggcatgctcg acgcgtttcg
     6061 ccaggtcagc ctgaccaagc gggtgccggt ggacgaggcc aggcgctacg gcctgtacca
     6121 cgatgacgac cccgacgccg cactctcggt cggcgaggat ggcctggtcg agatctcgcg
     6181 ctggcgtcac gcgatcgtca acttcccgca cccgctgttg aagcagggcc tggtgatcat
     6241 cgacacgccc ggcctgaacg cgatcggtac cgaacccgag ctcaccctga acctgatccc
     6301 gaacgcgcac gcggtgctgt tcatcctggc cgccgacacc ggcgtgacga ggagcgacat
     6361 cgaagtctgg cgcgaccata tcggcggcgg accggcgcgc atggtggtgc tcaacaagat
     6421 cgattcgatg tgggacgagc tgcgcggcgc cgaagaagtc gagcgccaga tcgccggcca
     6481 ggtcgcggcg gttgcgcaga cgctgtcgct ctccgaacgc caggtgtatc cggtatccgc
     6541 gcagaagggg ctggtcggca aggtcaacca ggacgcggcg ctgctcgcga agagccggct
     6601 gccgcagctg gaaagcgcgc tttccggcga actcatcccg agcaagcaac ggatcgtgcg
     6661 cgaacagctg catgccgaga tcggcgagct cacgtcggcc aagcaggcgc tcctggccgc
     6721 gcgcacgcgc aacgtggtcg agcagctggt ggagctgaag agcctgcgcg gcaagaacca
     6781 gaacatcatc gagcacatga tgaagcgcgt cgatctcgaa aagaaggagt tcgacgcgag
     6841 cctgctcaag ctgcagggca cccgcgcggt gtttacccgc ctgtccaccg aggtcttcac
     6901 gagcctcggc atggacgtgc tgaaggaaga catccgcagg acgcgcgagg cgatggatcg
     6961 cagcaagttc tccgccggcc tgcgttcggc tgtgcgtgaa tttttcgaac agattcgcaa
     7021 gaacctgacc gcctcgagcc agaagaccga cgagatcacc gagatgatga cgatcatgta
     7081 tcgccgcttc tcgtccgagc atggtctcgc gctgtccacg ccgatgccgt ttacgctgga
     7141 aaaatacctg cgcgaggtcg agatcattga aggcgtgtat cacaagcagt ttggcacggc
     7201 cacgctgctg accacccccc aggtggtcct gatgcgaaag ttcttcgatt cgatcgcgtc
     7261 gcgcgtcaag caaagcttcc tgcaggccaa tcgcgatgtc gaggcctggc tgaaggtggt
     7321 gatggcgccg ctggaagcgc aggtatggga gcacaaggcg cagctgaaga agcgccgcca
     7381 gtcgatcgag cgcattcaca tggccaccga cagcctcgag gaaaagctgg aggcgctcga
     7441 agcgatgcag gccgagctcg aggagcagaa gaaaacgctg ctgcagatcg aaggcgagct
     7501 caatgcggcg atcgcatccc agctgacctc gctgcgggct gcggcctaac cgcaagcttt
     7561 tgccatgaaa cgcgtagtcg accgcgcgcc gcacgcggcg gaaccgggca tttccctcgc
     7621 cgatccggaa ttttcacgca gtgtcatcga atggcagcgc agccacggcc gccatgctct
     7681 gccgtggcag aacacgcgcg acgcctaccg gatctggctg tccgaaatca tgctgcagca
     7741 gacacaggtc tccgccgtca ttccgtatta cctgcgcttc ctcgaacggt ttcccgatgt
     7801 cgcggcgctt gccgccggcg cgcaggagga tgtgatggcg cactggagcg ggctgggcta
     7861 ctactcccgc gcccgcaacc tgcaccgctg cgcgcagcgc atcgtggccg aatatggcgg
     7921 tagttttccg cgcgatccgg ccgcacttgc cgcgcttcca ggcattggcc gatcgaccgc
     7981 ggctgcgatc gcggcattcg cctatggcga gcgggcggca atcctcgacg gcaacgtgaa
     8041 gcgggtgttc tgccgggtgt tcggcatcga agggtttcca ggtgcgaagc cgatcgaaaa
     8101 tgcgctctgg gaacgcgcac aggccttgct cccggagcag ggcgtggaag cctacacgca
     8161 gggcctgatg gacctcggcg cgacagtctg cacccgtgga aagccttcct gcgcgcgttg
     8221 tccgctttct tcgcgctgcg acgcctatgc gagcaaccgt acccatctgt tgcccaccgc
     8281 ccgccccgcg aaggaggtgc cgcggcgcag cgtcgcgatg ctggtgatcg aagcgggcga
     8341 gcacgtgctg ctggaacagc gtccggacag cggcatctgg ggtggcttgc tgtcgttgcc
     8401 ggaaataccg gcagagacgg ccgaggaggg gagggtgacg caggcggccg cgcgcttcgg
     8461 ccatgtggaa gcggtcgaac ggctgcagcc gttcacgcat gtgttcaccc actttcgcct
     8521 ggatgtcctg ccatttcgcg tgcggctgac gcagccggtc acctttgccg gagaagtgcg
     8581 gtacgtctgg tctgcgcaca agggggtggc cgatgccgcg ctgccggcgc ctgtgaaaaa
     8641 actgctgcta tcgctgtatt cgcgtaccgg gttccaggcg cagctggtct agtccaccca
     8701 ctccacgcga ttgcgcccca gctgcttggc ctggtagagc gccgagtcgg cgcgcgagat
     8761 cagatcgtcg atgcgttcgc cgcgccggta ctgggccacg ccgatggaca gcgaaatctt
     8821 tccttgttcc agtaccttgc cgaagtcgag gaagtgggcg tacacgcgca ggcgttcggc
     8881 gtgcatcacg gctcccttga cgtcggtgtc atgcaggatc tgcatgaatt catcgccgcc
     8941 atagcgtccc agcacgtcgg cctcgcgcac gctctcgcgt gccatccggg aaaactcgcg
     9001 caggatgatg tcgcccgcaa gatggccgtg gctgtcgttg atatccttga agtgatcgac
     9061 gtcgatcagg cacagcgaga aagtctggcc gtggcgatcg gcccgcagct tcgcctggtt
     9121 cagctcggtc tgcagctggc ggcggttgaa caggccggtc aactcgtcgc gcgccgcctt
     9181 ttcctcgaaa tgctgcagct cgctgcgtgt gctgcgcagg gtctggcgca ggttgtggat
     9241 ctggcgcgcg acgagaatca tgccgggcag ggtaagcgcc agcacgcacc attgcacgag
     9301 gtcggaatga aagccgaaat cgaaaccctc ggcgcgggcg cgcaccacga tcatgcccag
     9361 ataggcgagc aggaaagcgg tcgcgagcag ggtcagcgac gccgccgaga ggcgaaagag
     9421 tccgaatgaa aatgcgatca gcgcaaacgg cacgagtgcc acctgggttg cgcgttccat
     9481 ccatgccacg cccagcatca acacgagcga ggcgaacaac tggggcaaca tcagggcggg
     9541 gtcgtccagc cggcggttcc atcccttgtg gaagacgagc tggaacattc caatgatcag
     9601 ggcggccgca ccggcggcgc tggcgagcgt gacgcggtcg atgaagccat ggcggaagaa
     9661 cacaaacagg ctggccacat acagcgctcc cgtcagcgcg accggcgctg cctgcggcag
     9721 ggtcgagccg gactgaggct tgcggcggtc cagcgactcc ggccgcgttg cttcgcgtgc
     9781 gtcatccagg aacatacgat tggcgctcaa gtcaggtccg tggcgtgggt aaaggggcga
     9841 tgctcaggct gatgcgcatc gtcgacgctg tctggtacgc gggaagatgc gttcgagcat
     9901 ttatcctatg aacgcgtcgg tcgccgtttg cgcggtgaca aatcggatca atataggcgt
     9961 gattcggacc ggagaacccg ctgtgcgcgc ggtcttctat tgccggactg gcaatcagat
    10021 gatcttgtgc tgctgcaggt actggtaaac cagcgagagc cgggcgcccg gatcctcgag
    10081 ctcgagcagc ttctggcgtg ccttgagcgg gatagggagg atctcgcacc agcggttggc
    10141 gacccaggcg gcactgtcga gacggatcgg gtacgcgaac gggctgacga actctccgtc
    10201 ctgtgcggcg cgggcgcggc tctcgatgtc gtcgatgacg atcttgagcg tcttggcgca
    10261 gcggacatgg atgtcgctga cggggatttc cggatccggc tcgatggctt cgacgcgcgc
    10321 ttcaagccgc tggtccgcgc gcacccgggt ttccaggacg cgaaagcgct cgccgccctg
    10381 cacccgcagg tgcatcacgc cgagttcctg catgtcccag tcggtgatcc gggcaaggca
    10441 tccgaccggc tccggttcgg ccgcggcgcc gacttcctgt ccgttccgga tcaacgccac
    10501 gccgaacggt gcgccggtct tcatgcaatc gcgcaccatg tcgatgtagc gcgtctcgaa
    10561 gatgcgcagg gggagaatgc cgtccgggaa gagaactgtc ttgagaggga aaagcgggag
    10621 ccagccgagg tcgttgggca taggttcagt tcagttcccg gtgtcgcagc agcacccgtt
    10681 cggcgccgcc gctgaagtgg cgcgcgagtt tttccaccat gtagacggaa cggtgctggc
    10741 cgccggtaca cccgattgcg accgtcaggt agctgcggtt gtcctgccgg aaggcgggca
    10801 gccatttttc gacgaagccg cgtatgtcgg ccagcagctc gccgacctgt tcctgcgcat
    10861 cgaggaaggc caccaccggg gtatcgcgac cggtgaacgg gcgcaggttg gtgtcgtaat
    10921 agggattcgg cagcacgcgc acgtcgaaca ccaggtccgc gtcgagcggc acgccgaact
    10981 tgaacgcgaa ggactcgaac agcagcgtga gcggggagcg ttcgttgccg accaggtcct
    11041 tgatccagct gcgcagcttg ttggccgaca gtccggatgt gtcgatgaca tggccgaggc
    11101 cctcgatcga cgacagcatc tcgcgttcct gcaggatgca ttcggtcagc gtcggctgtc
    11161 ccccgttagg ctgggcgctg cgcaacctgt gcgaaagcgg gtggctgcgg cgggtctcgg
    11221 aaaagcgggt gatcagggat tcggtcttcg ccgtcaggaa cagcaccttg acgtcatggc
    11281 cctgttcctt cagcgtgcgg atgtcgccgg gcaggtcggc gagggaatcg gcgctgcgca
    11341 cgtccacagc cacagcgagc gct
//
LOCUS       DATLGF010000156         5937 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_85220,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000156 DATLGF010000000
VERSION     DATLGF010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5937
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85220"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2299)
                     /locus_tag="VM406_04710"
     CDS             complement(<1..2299)
                     /locus_tag="VM406_04710"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVL75295.1"
                     /translation="MTPPADASERHPRLRHALASFARPLRSPAFGWLVGALLILGVGW
                     LVLLQHLAADRRTAEDAALRSASILAEAYASHLQQSLEAVDQVALYLKFAWEYSGGTF
                     RLENIEAANVLPPGSDFVAAIVDRNGRIISSTVPLAGKPLDARHEPFFRAHLGVPVDF
                     FYIGPMRPGTLNGKTVVQFSRQLIDKNGVFAGVVLISTPPEYFIANYDEVTLGQHGLL
                     AILGGAGTVRMIRVGDRISGPAGGTLLAAPPLEGSKGRSFLEGSRWFLDGRSRYAAWN
                     DTRAYDMVALAAIDQETALLPHMQRRASLITGAIAASSALLLAALVATLFSAQLANRQ
                     QQLEALRRSYRAATEGGGDGYYIARPLFDATGKVSDFETVDCNARGAQFFEMRREELL
                     GKRASGLTDAGERRRVLANLTTAYESGIFEGEQPWRIGTHVRWVHLRAIRSNDTVAIT
                     ARDITATKEYLGELERRGNEDALTGLPNRHWVKDYLPAAMERTDRERRRLAVLFIDLD
                     GFKAVNDTMGHDAGDEVLRNAGLRLRDAVRPHDKVVRIGGDEFIVVLEDVGAASDAAH
                     VAERIVHAFQEPFRLSKGTHSIGTSIGIAVYPEDGPDADTLLKHADIAMYSAKTSGKQ
                     RYHFFDEEFSIALRIRHENEAELRAALERNELVVHYQPRIDLHTGRSSSLEALVRWQH
                     PQRGLIHPADFIPIAEDSGLIVPLGQQVIDKVCAQLALWGRQGKELVPVSINVSARQF
                     QEIDVAAALAEALQRYGVAPSLIELE"
     gene            complement(2658..4343)
                     /gene="yidC"
                     /locus_tag="VM406_04715"
     CDS             complement(2658..4343)
                     /gene="yidC"
                     /locus_tag="VM406_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HVL75296.1"
                     /translation="MDIQRTILWVVFSLSLLLLWDNWMRYSGHQSMFFPSPAPTQQAE
                     PGAAPANGAAPRADVPQATMPATAAPAAVPGTTAPAASETITITTDRYRAEIDTVGGE
                     IKRLELLGHRDTVDPNRNVVLLDNTAARTYVAQTGLIGGPFPNHRSTFTARPGPRELQ
                     NNELQLVMESTQDGVTVVKTYTFHRDSYVIDVRHDVVNNSGAPIAPSVYLQIMRDGNK
                     PAGESALYMTFTGPAIYTEAEKFQKIDFDDVAKGRAKHVTRADDGWVAMVQHYFVTAF
                     VPPEGVTRENFTRRIDNNLYAVGSIIPMGTVEPGATASMDSRLYSGPQESARLVEVAE
                     GLDLVKDYGWLTILARPIFWLMDQIHKVLGNWGWTIIALTVLIKLAFFPLSAASYRSM
                     AKMKTVTPKLQSIRERYKDEPQKMNQAMMELYKTEKINPLGGCLPILVQIPVFIALYW
                     VLLASVEMRNAPWFWINDLSSPDTLFTIPGVNMPIGALPIIMAITMFIQVKLNPTPPD
                     PIQAKVMMAMPIVFSVMFFFFPAGLVLYWVVNNILSIAQQWVITRKIEAGKPA"
     gene            complement(4366..4635)
                     /gene="yidD"
                     /locus_tag="VM406_04720"
     CDS             complement(4366..4635)
                     /gene="yidD"
                     /locus_tag="VM406_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HVL75297.1"
                     /translation="MKTLLLLLLRAYQLAISPFLGRNCRFYPSCSAYAIEAIRVHGSA
                     KGSYLAARRLCKCHPWHPGGVDPVPGTADENPDSRRARRKTPDHA"
     gene            complement(4643..5158)
                     /locus_tag="VM406_04725"
     CDS             complement(4643..5158)
                     /locus_tag="VM406_04725"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HVL75298.1"
                     /translation="MKPADSQAAAPPAVPPAAPATPANLHAYPRAARIVRTDEFSSVF
                     HLRPVRRSAHFMLYVRPTALPRARLGIVAAKRFAPRAVTRNTIKRITREIFRQSSLPG
                     GDCIVRLTRPVNSKADPATSTRLKTTLRSELHDLLHGYFKAAKQPEKPSEKQSEKRPD
                     QPPENPPGNRS"
     gene            complement(5174..5308)
                     /gene="rpmH"
                     /locus_tag="VM406_04730"
     CDS             complement(5174..5308)
                     /gene="rpmH"
                     /locus_tag="VM406_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HVL75299.1"
                     /translation="MKRTYQPSVVRRKRTHGFRVRMSTRSGRAVLNARRAKGRKRLAV
                     "
BASE COUNT          984 a         1869 c         1984 g         1100 t
ORIGIN      
        1 gctccagttc gatcagcgac ggtgcgacgc cgtaccgctg caacgcctcg gcaagcgccg
       61 ccgctacatc gatttcctga aactggcgcg ccgacacatt gatcgacacc ggcaccagct
      121 ccttgccctg cctgccccac agcgcgagct gcgcgcacac cttgtcgatc acctgttgcc
      181 cgagcggcac gatcaggccg ctgtcctcgg cgatcgggat gaaatcggcc ggatggatca
      241 gcccgcgctg cgggtgctgc cagcgcacca gcgcttccag gctggagctg cggccggtat
      301 gcaggtcgat gcgcggctgg taatgcacga ccagttcatt gcgctccagc gcggcgcgca
      361 gctcggcctc gttttcgtgc cggatgcgca gcgcgatcga gaactcttcg tcgaagaaat
      421 ggtagcgctg cttgccggaa gtcttggcgg agtacatcgc gatatccgca tgcttgagca
      481 gggtgtccgc atccggcccg tcttccggat agaccgcgat cccgatcgag gtgccgatcg
      541 aatgcgtgcc cttgctcagc cggaatggtt cctggaacgc gtgcacgatg cgctcggcca
      601 catgggcggc atcgcttgcc gcgccgacgt cctccagcac cacgatgaac tcgtcgccgc
      661 cgatgcgtac caccttgtcg tgcgggcgca ccgcatcgcg caggcgcagt ccggcgttgc
      721 gcagcacctc gtcgccggcg tcatggccca tcgtgtcgtt gaccgccttg aagccgtcga
      781 ggtcgatgaa cagcaccgcc aggcggcgcc gctcgcggtc ggtgcgttcc attgcggccg
      841 gcagatagtc cttgacccag tgacgattcg gcaggccggt cagcgcgtcc tcgttgccgc
      901 ggcgttcgag ctcgcccagg tattccttcg tggcggtgat gtcgcgcgcg gtgatcgcga
      961 ccgtgtcgtt ggagcggatt gcgcgcaagt gcacccagcg tacatgcgtg ccgatgcgcc
     1021 atggctgttc gccttcgaag attccgcttt cgtaggcagt cgtcaggttg gccagcacgc
     1081 gccggcgctc gccggcgtcg gtcaggccgg aggcgcgctt gccgagcagc tcctcgcgcc
     1141 gcatttcgaa gaactgggcg ccgcgcgcat tgcagtccac cgtttcgaag tccgaaacct
     1201 tccccgtcgc atcgaaaagc ggcctggcga tgtaataccc gtctccgcct ccctcggtgg
     1261 cggcccggta ggagcgccgc agcgcctcca gctgctgctg gcggttcgcc agctgggcgg
     1321 aaaaaagggt agcgaccaga gcggcgagca gcagtgccga ggacgcggcg attgcgccgg
     1381 tgatcagcga ggcgcggcgc tgcatgtgcg gcagcagcgc ggtttcctgg tctatcgccg
     1441 ccagcgcgac catgtcgtag gcgcgtgtat cgttccacgc ggcatagcgg ctgcggccgt
     1501 cgaggaacca gcggcttcct tccaggaagc tgcgtccctt cgacccttcc agcggcggcg
     1561 ccgcaagaag ggtgccgccg gccggtcccg agatcctgtc gccgacccgt atcatgcgca
     1621 ctgtgccggc cccgccgagt atcgccagca gcccgtgctg gcccagcgtg acctcgtcgt
     1681 agttcgcgat gaagtattca ggcggcgtgg atatgaggac gacccccgcg aacactccgt
     1741 tcttgtcgat caactggcgg gaaaactgta cgaccgtctt gccgttcagc gtcccagggc
     1801 gcatcggacc gatgtagaag aagtcgaccg gcacgccgag atgcgcacgg aaaaacggtt
     1861 cgtggcgggc gtcgagtggc tttccggcga gcggaacggt gctggagatg atgcgcccgt
     1921 tgcggtccac gatcgcggcg acgaagtccg agcccggcgg caggacgttg gcggcttcta
     1981 tgttttcgag gcggaaggtg ccgcccgagt attcccacgc gaacttcagg tatagcgcca
     2041 cctggtccac ggcctccagc gattgctgga gatgactcgc atacgcttct gccaggatcg
     2101 acgccgagcg cagtgccgcg tcttccgccg tgcgcctgtc cgcggccagg tgctgtaaga
     2161 gcacgagcca gccgacgccg aggatgagca gcgcgccgac cagccagccg aacgcgggcg
     2221 agcgcaacgg tcgcgcgaag ctcgcgagcg cgtgccgcag ccgagggtgg cgctcggagg
     2281 catctgccgg gggcgtcatc gaaatcgcca ttccttcctt cgcgggctgc tgtcttggcc
     2341 gtattgcatc atcgacccgt tgcgtcaacc gccgggctgg cgcaagaggt cgatgtcgtg
     2401 ggaggcgtcg cccgaaggag caaggtgggg gaagggctcc gcttgtcatt tatcgggtcc
     2461 gcagcgtttc ccttcaatac tgccgggctg cctgaggggc ggccacaaac cttgctgaga
     2521 acgggggcgg acccggatag gaatcttttg gcatggcgaa gtggcaaaga gttccgcagg
     2581 tcgctgaacg cgccggtcgc attgcacggg cgaaaaaaaa cccgcgcagg gcgcgggctt
     2641 cttcgggcgg ggcggggtca cgccggcttg ccggcctcga tcttcctcgt gatcacccac
     2701 tgctgcgcga tcgacaggat gttgttcacg acccagtaca gcacgaggcc ggccgggaag
     2761 aagaagaaca tcaccgagaa cacgatcggc atggccatca tcaccttggc ctggatcggg
     2821 tccggcggcg tggggttcag cttgacctgg atgaacatcg tgatcgccat gatgatcggc
     2881 agcgcgccga tcggcatgtt gacgccggga atggtgaaca gcgtgtccgg cgaggacagg
     2941 tcattgatcc agaaccacgg cgcattgcgc atttccaccg atgccagcag cacccagtac
     3001 agcgcgatga acaccgggat ctgcaccagg atcggcaggc agccgccgag cgggttgatc
     3061 ttctcggtct tgtacagctc catcatcgcc tggttcattt tctgcggctc gtccttgtaa
     3121 cgctcgcgga tagactgcag cttcggcgtg acggtcttca tcttcgccat gctgcgatac
     3181 gacgcggccg acagcgggaa gaaggccagc ttgatcagca ccgtcagcgc gatgatggtc
     3241 cagccccagt tgcccagaac cttgtggatc tggtccatca gccagaagat cggccgcgcg
     3301 aggatggtga gccagccgta gtccttcacc aggtccagcc cctcggccac ctcgaccagg
     3361 cgcgcggatt cctgcgggcc cgaatacagg cgcgagtcca tcgaggcggt tgcgcccggt
     3421 tccaccgtac ccatcggaat gatcgatccg accgcataca ggttattgtc gatgcggcgc
     3481 gtgaagttct cgcgcgtgac gccttcgggc ggcacgaagg cggtgacgaa atagtgctgc
     3541 accatcgcca cccagccgtc gtcggcccgg gtcacatgct tggcgcgtcc cttggccacg
     3601 tcgtcgaagt cgatcttctg gaatttctcg gcctcggtgt agatggcagg gccggtgaag
     3661 gtcatgtaca gcgcggactc gccggccggc ttgttgccgt cccgcatgat ctgcaggtag
     3721 accgacgggg cgatcggcgc gccgctgttg ttcaccacgt catggcgcac atcgatcacg
     3781 taggagtcgc gatggaaggt ataggtcttc accaccgtca cgccatcctg cgtcgattcc
     3841 atcaccagct gcagctcgtt gttctgcagc tcgcgcgggc ccggccgggc ggtgaaggtg
     3901 gagcggtggt tcgggaacgg gccgccgatc aggccggttt gcgcgacata ggtgcgcgcg
     3961 gcggtattgt cgagcagcac cacgttgcgg ttcggatcga ccgtgtcgcg atgaccgagc
     4021 agttcgagcc gcttgatctc gccgcccacc gtgtcgatct cggcgcggta ccggtcggtc
     4081 gtgatcgtga tggtctcgct tgcagccggc gcggtggtgc cgggcacggc ggccggcgcc
     4141 gccgttgccg gcatggtcgc ctgcggtacg tcggcgcgcg gtgcggcgcc gttggcagga
     4201 gccgcgcccg gctcggcctg ctgggtcggc gccgggctcg ggaagaacat cgactggtgc
     4261 ccgctgtagc gcatccagtt gtcccacagg agcagcagcg acaacgagaa cacgacccac
     4321 aggatggtac gttgaatatc catatctaat agttatcgag atcgattaag cgtgatcggg
     4381 agttttgcgg cgtgcgcggc gagaatcggg gttctcgtcc gccgtgcccg gaacaggatc
     4441 gactccgccg ggatgccagg gatgacactt gcacaggcgc ctggccgcca gataactgcc
     4501 cttcgccgag ccgtgcacgc ggattgcctc gatggcgtag gcggagcagg acgggtaaaa
     4561 acgacagttc cggcccagga acgggctgat tgccagctgg tacgcgcgca atagtagcag
     4621 caaaagcgtt ttcatcgggc attcatgagc gattgccggg aggattttcc ggcggctggt
     4681 cgggccgctt ctcggactgc ttctcagatg gcttctcagg ctgcttggca gccttgaaat
     4741 agccatgcag gagatcgtgc aattcggacc gcagggtcgt cttcaggcgt gtcgaggtgg
     4801 cgggatcggc cttgctgttg accggacggg tcaggcgtac gatgcagtcg ccgccgggca
     4861 gcgaggactg acggaagatt tcgcgcgtga tgcgcttgat cgtattgcgt gtgaccgcgc
     4921 gcggcgcaaa tcgttttgcg gcaacgatgc ccaggcgcgc ccgcggcagc gcggtcgggc
     4981 ggacgtacag catgaagtgc gcgctgcggc gcaccgggcg caaatgaaaa acggatgaaa
     5041 actcatccgt tcttacgatc cgcgcggcgc gcggataggc gtggagattc gctggagtcg
     5101 caggggctgc aggaggaact gccgggggag ctgcggcctg cgaatccgcc ggtttcacgc
     5161 tgttgctgcg gactcagaca gccaggcgct tgcggccctt ggcgcggcga gcgttgagta
     5221 cggcacggcc cgagcgggtc gacatgcgga cgcggaaacc gtgggtgcgc ttgcggcgga
     5281 cgacggaggg ttggtaggta cgtttcatgt tgttctcgct aactgttcag cagaaatggg
     5341 tgggttcgtc acgtcggccc attcttgttt tggtgaggca cgcaaaacga aacaggcagt
     5401 gaacccgcga ttagacactg ttttctgctt ctgtgtcaag cacttaggcg cggcgatggc
     5461 gttgtcgatc tgctctggag gtgggttctg gcgacccttg caatgcgttc acccgtcgct
     5521 cgatccccaa acgttgtcat cctgagccgc ccgcgaagga tcccgcgcgc ctccgcgggc
     5581 tccgcccgtt gctgcaggga tgaggtcctt cgcttgcgct caggacgaca gctcgtggta
     5641 gtccgtccgc gctgccattt ctcatcccga gccgcaggcg agggacctca tccgttcgtc
     5701 ccgcggcgtc ccacggttgc ccccgtcatc ccccttgcct actacgtcca aaccccccgg
     5761 ttttgcaagc acttttttat acgacaaaac tgtttcaacc tgtggataac tcgccgcctt
     5821 cgggtataga atagccctcc cgcgcggccc gcccctggcg gttgcttgtc ggtacggcaa
     5881 tttttcccgg tgcaagtccc ggaccccgca actgccggcg gcgagcaatt ccaaggc
//
LOCUS       DATLGF010000157         3154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_38523,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000157 DATLGF010000000
VERSION     DATLGF010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38523"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1436
                     /locus_tag="VM406_04735"
     CDS             <1..1436
                     /locus_tag="VM406_04735"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVL75300.1"
                     /translation="PELVQRLRFAPALPTPPSTALRVIELANDPSANLMQIADCVALD
                     PALAAKMLKVANSPLYNARRGVANVRQAVSLLGTRAAISVALSFSLVPGLRGASAEAA
                     RFWRRSVLAAHACRLLAQHCKLNPDDALPAGLLQDIGILAMDAVLGEPYRNIADAAVD
                     QDALVEAERETFGAGHDEVGYWLLKRWNLPDQLALACLTSHVTPPAERDSPTIAACVA
                     VSGYIADIFLNSGIAVSVLKAGNSARHWLGIEADAFGQLIEEMSASAESLEELFELDL
                     IDHAQAAALAAEARELMLVANLGQHRELEEKARRDALTGAHNRSYFEDALAQEFGVAS
                     RHGWPLSLAFLDIDHFKRINDQYGHAVGDSALVALARLISAQIRTGDIFARYGGEEFL
                     LLFPGTPVESATLVLQRIRETVERFEHPIGVDQTFRLTVSAGLACHTQGSTVYMSAAE
                     LLAAADQALYAAKRAGRNRIETAPPPR"
     gene            1490..2587
                     /locus_tag="VM406_04740"
     CDS             1490..2587
                     /locus_tag="VM406_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid 1,2-hydratase"
                     /protein_id="HVL75301.1"
                     /translation="MPLLNGLRVVPWMQLCIAALLTAMVAPAAAAGFAAVTPGKPLSF
                     PRDFGAHPDFRTEWWYATGWLTLPDGKPLGFQVTFFRSATEHDRANPSRFAPHQIIIA
                     HAALADPQHGRLIHDEKSARAGFGMAFAREGDTDVKLGDWRFVREADGRYRASIPARD
                     FTLELSLLPTQPPMLQGERGYSRKGPQPQQASYYYSQPQLRVDGTVTRAGERLAVTGE
                     AWLDHEWSTTVLDPSAEGWDWIGINLDGGGALVAFRIRTRDGGDLWAYAALRAPDGSV
                     QQFGPEQVSFSPRRLWRSPRTDASYPVAYTVTAGPHTWQIDSLMDDQELDSRATTGAV
                     YWEGAVTVSQDGRPVGRGYLEMTGYVSPIQL"
     gene            2584..>3154
                     /locus_tag="VM406_04745"
     CDS             2584..>3154
                     /locus_tag="VM406_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HVL75302.1"
                     /translation="MTPTSSERAAGSLATLKRLLPFLAPYRMQFMLAGMALLVAAGAT
                     LAVPYAFRQMIDVGFGPAIVGHAAAEQLGSREHIDLYFLALFGVACVLAVATAARFYM
                     VSWLGERVTADLRAAIYRHVITQSPQFFEITRTGEVLSRLTTDTTLIQALVGTSISLA
                     LRNALLFVGGLVMLFVTSPRLSAIILVLLM"
BASE COUNT          496 a         1119 c         1014 g          525 t
ORIGIN      
        1 cccccgaact cgtgcagcgc ctgcgcttcg ctccggcctt gccgactccg ccctcgacgg
       61 cgctgcgcgt gatcgaactg gcgaacgacc cctccgccaa cctgatgcag atcgccgact
      121 gcgtggcgct cgatcccgcg ctggcggcaa aaatgctcaa ggtggccaat tccccgctct
      181 acaacgcacg gcgcggcgtg gccaacgtgc gccaggcggt gagcctgctc ggcaccaggg
      241 cggcgatctc ggtcgccctg tcgttctcgc tggtgcccgg actgcgcggc gcttcagccg
      301 aagccgcgcg cttctggcgc cgctcggtgc tcgccgccca cgcctgccgc ctgctcgcac
      361 agcattgcaa gctcaatccc gacgacgcgc ttcccgccgg cctgctgcag gacatcggca
      421 tcctggcgat ggacgcggtc ctcggcgagc cttaccggaa tatcgccgat gcagcggtcg
      481 accaggacgc gctggtcgaa gccgagcgcg aaaccttcgg agccgggcat gacgaagtcg
      541 ggtactggct gctcaagcgc tggaacctgc cggaccagct ggcgctggcc tgcctgacca
      601 gtcatgtgac gccgccagcc gagcgcgaca gtccgaccat tgccgcctgc gtagccgtgt
      661 ccggctatat agcggacatc ttcctcaaca gcggcatcgc ggtcagcgtg ctcaaggcgg
      721 gcaacagcgc gcgccactgg ctgggcatcg aagccgatgc gttcggccag ctgatcgagg
      781 agatgagcgc gtcggccgaa agcctggaag aactgttcga actcgatctc atcgaccatg
      841 cacaggcggc cgcactggca gccgaggcgc gcgagctgat gctcgtcgcc aatctcggcc
      901 agcaccgcga actagaggaa aaagcgcggc gcgacgcgct caccggcgcc cacaaccgtt
      961 cctatttcga ggacgcgctc gcgcaggaat tcggcgtcgc cagtcgccat ggctggccgc
     1021 tgtcgctcgc cttcctcgac atcgaccatt tcaagcgcat caacgatcag tacggccacg
     1081 cggtcggcga cagcgcgctg gtcgcgctgg cgcggctgat ctcggcccag atccgcaccg
     1141 gcgatatctt cgcgcgctac ggcggggaag aattcctgct gctgtttccg ggcacgccgg
     1201 tggagtcggc caccctggtg cttcagcgca tcagggagac ggtcgagcgc ttcgagcacc
     1261 cgatcggcgt cgaccagacc ttccgcctga ccgtctctgc gggactggcc tgccatacgc
     1321 agggcagcac cgtctatatg tctgccgccg agctgctggc cgcggccgac caggcgctgt
     1381 acgcggccaa gcgcgccgga cgcaaccgca tcgagaccgc gccgccgccg cgctgagctt
     1441 gcgagcgcaa tagcacggat aatcgctcct tcttatggag cgaatgaaca tgcctctact
     1501 caacggactg cgcgtcgtac cgtggatgca actgtgcatt gccgcactgc tgacggccat
     1561 ggtcgcgccg gccgcggccg ccggctttgc cgcggtcacg ccgggcaagc ccctaagttt
     1621 tccgcgcgat ttcggagccc acccggattt ccgcaccgaa tggtggtacg cgaccggatg
     1681 gctgacgctg cctgacggca agccgctcgg tttccaggtc accttcttcc gctccgccac
     1741 cgagcatgac cgagccaatc ccagccgctt cgcgccgcac cagatcatca ttgcccacgc
     1801 cgcgcttgcg gatccgcagc acggccgcct gattcacgat gaaaaaagcg cccgcgccgg
     1861 ctttggcatg gcgtttgcgc gtgaaggcga taccgatgtg aagctcggcg actggcgctt
     1921 tgtgcgcgag gccgacggcc gctaccgcgc cagcattccg gcgcgcgatt tcacgctgga
     1981 actaagcctg cttccaacgc aaccgccgat gctgcagggc gaacgcggct actcgcgcaa
     2041 gggtccgcag ccgcagcagg cgagctacta ctacagccag ccgcagctgc gggtggacgg
     2101 caccgtgacg cgcgctggcg aacgccttgc ggtgacaggc gaagcctggc tcgaccatga
     2161 atggtcgacc accgtgctcg acccgagcgc cgagggctgg gactggatcg gcatcaatct
     2221 cgacggcggc ggtgcgctgg tcgccttccg catccggacc cgcgacggcg gcgacctttg
     2281 ggcctatgcc gcgctgcggg cgccggacgg cagcgtccag cagttcggac cggaacaggt
     2341 atcgttcagc ccgcgccgac tatggcgctc accgcgcacc gacgcaagct atccggtcgc
     2401 ctacacggtt actgccggcc cgcatacctg gcagatcgat tccctgatgg acgaccagga
     2461 actcgattcg cgcgcgacca ccggcgcggt ctactgggaa ggcgcggtga cggtctcgca
     2521 ggacggccgg cctgtggggc gcggatacct tgaaatgacg ggctacgtga gcccgatcca
     2581 gctatgaccc cgaccagctc cgagcgcgcg gccggcagcc tcgcgacgct gaagcggttg
     2641 ctgccgtttc tcgcgcctta ccgcatgcag ttcatgctcg ccggcatggc cctgctcgtc
     2701 gcggccggcg cgacgctggc ggtgccctat gcgttccgcc agatgatcga tgtcggcttc
     2761 ggtccggcca tcgtcggcca tgccgccgcc gagcagctgg gcagccggga gcacatcgac
     2821 ctgtattttc tcgcgctgtt cggtgtggcc tgcgtgctgg cggttgccac cgccgcgcgc
     2881 ttctacatgg tttcctggct gggagagcgc gtgaccgccg acctgcgcgc ggcgatctac
     2941 cggcatgtga tcacgcagag tccgcagttc ttcgagatca cgcgcaccgg cgaggtgctc
     3001 tcgcgcctga ccaccgacac cacgctgatc caggcgctgg tcggcaccag tatctcgctg
     3061 gcgctgcgca atgcgctttt gttcgtcggc ggactggtca tgctgttcgt gaccagcccg
     3121 cgcctgtcgg ccatcatcct ggtcctgctg atgc
//
LOCUS       DATLGF010000158         6141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_225093, whole genome shotgun sequence.
ACCESSION   DATLGF010000158 DATLGF010000000
VERSION     DATLGF010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225093"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="VM406_04750"
     CDS             <1..948
                     /locus_tag="VM406_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HVL75303.1"
                     /translation="LLHSAPNDQMTAFEYKEMQQAHMGLRGNAYSFIERDGRGRIQSL
                     LPQDPDKVTVRRNPSDGAIVYDVSGVENGIPARRMFHPRGFSLDGLIGLNPIALARET
                     IGLAIAGEKAGAEAVGRGIVPPAALEVPGTPDKKQRADIRESWREIHQGNKNDIAVIG
                     GNMKLHDLRINMADMQFMESRKFQVTEIARMFRIPPHMLADLERATFSNIEHQDLQFV
                     KYTLLPWLVRWEQRANLSLLSADEREEGYYVKFNAAALLRGDMKARFESYKMGRDMGV
                     YNANEIRGLEDLDPYEGGDTYLEPLNMQRVGEPREEEQE"
     gene            945..1868
                     /locus_tag="VM406_04755"
     CDS             945..1868
                     /locus_tag="VM406_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HVL75304.1"
                     /translation="MKKAYLAELVFNTPLAIHGAKLEVILNVLKGHLNIEDIGAFDAS
                     SLPAGLNAAKQGETTAYDLGHGVAVLPVHGSLTHRTRGLDAMSGLHSYKQMSMDFRAM
                     LNDSSVRHIVLDVNSPGGHVNGLFDLADEIFAARGTKPISAIVDESGYSAGYAIASAA
                     DEIIVPRMGGVGSVGIVAAHVDKSAAMEREGVKVIPIYAGARKADGLPGVPLTDEAHA
                     RLQAEVDLAYELFVETVARNRGMSVEAVKATEAGTYRGADAVRVRFADRVMTANDAIR
                     EIVAKNAGGSAARNSNGRVQRAAQAMRIRSA"
     gene            1945..3213
                     /locus_tag="VM406_04760"
     CDS             1945..3213
                     /locus_tag="VM406_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HVL75305.1"
                     /translation="MKDINSLLQRRAGVQARVAELAALESAGTELTAEQLAEFGALQA
                     EFDDLSAKIGRIQSAERMAAVTATPLTGLTVPAVPKAAKQQGDDIGMIVRSLCASKGD
                     PRAAANFADTHGYPELAAALNTGTNSAGGFIVPPGYVPEFIEMLRPRSVVRASGARVV
                     PMPNGTLTMPKLTGSSTATYQGENDDITPSQPAFGQMTLTRKKLTALVPVSNDLIRFS
                     SPQANTIVRDDMLQSIGLREDIGFLRDDGTGNLPKGLRHWAPAGNVIAANATINLQNV
                     KNDLGKLELALLSNNVRMIAPGWVFTPRTMVFLQNLLDGNGNKAFPEIDAGMLRGKPY
                     KLTTQIPENLGAGSNESEIYLVDFNEVIIGEATGLIIDVSSEASFKDGANMVSAFSQD
                     LTLVRAITEHDIQPRHNAAVAVLTAVRWTP"
     gene            3297..3470
                     /locus_tag="VM406_04765"
     CDS             3297..3470
                     /locus_tag="VM406_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75306.1"
                     /translation="MKKVVIKFTATVSPYQTGEIAGFDEETAKKYIAAKLAVEYKADK
                     KSADDGAGDGSQK"
     gene            3598..4167
                     /locus_tag="VM406_04770"
     CDS             3598..4167
                     /locus_tag="VM406_04770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HVL75307.1"
                     /translation="MNLRLVTPPDGELIPLNEVKTHLRVDSALTADDGLIQSLVTAAM
                     QTAEHRTGRSLLPQTWELALDRFPEKIALDKPPIIEVESVKYVDANGDLQILPESDYL
                     LDDFGEPGRLIPAYSKSWPMTREQPNAVRIRFRAGYENAAAVPQAIKQWILLTVGTMY
                     ENREGVVVGQSVQSLPHIDGLLDRATVWR"
     gene            4169..4513
                     /locus_tag="VM406_04775"
     CDS             4169..4513
                     /locus_tag="VM406_04775"
                     /inference="COORDINATES: protein motif:HMM:NF017343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HVL75308.1"
                     /translation="MAAGRLNKRLRIRAWQDNPSGMADIEPTYSAGMEVWAGVEPTRG
                     SVYYGAKQTGDDVTHLITMRLSPTVNERTVTPSHVIDDEVAGTRYRVKRSMDLKGQGR
                     WLQIEAELLGAI"
     gene            4513..4971
                     /locus_tag="VM406_04780"
     CDS             4513..4971
                     /locus_tag="VM406_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75309.1"
                     /translation="MLEVNVKLAGHTALDFDKKKMRRALRQIGGGVRKEARRLVARRA
                     VSQPGQDPGRDSGTLSRSIKVKVFSSGMGAVVSPQKTAEMGDAFYPAFLVYGVRRDAK
                     RRKDHQRQVDNGKGWRMAPRNDFMMSAQFKRSDVSKAALMEALKDALKPR"
     gene            4968..5516
                     /locus_tag="VM406_04785"
     CDS             4968..5516
                     /locus_tag="VM406_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75310.1"
                     /translation="MNLNIVIAHLRARVPEFANRVAGAAQFSGIPESANLPVPAAYVI
                     PMDESPENNVSQNGYRQRVREGFAVVVVLSNTPDERGQAAALTVHDMRAKLFRALLGF
                     EVGEEYGGIEFDGGNLLRMDRARMYYQFEFAADYEIGEEDTWLDVRDSDLPDWEGVDI
                     DVDLSMPDGQIDATLSIDLPHQ"
     gene            5526..5696
                     /locus_tag="VM406_04790"
     CDS             5526..5696
                     /locus_tag="VM406_04790"
                     /inference="COORDINATES: protein motif:HMM:NF022401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HVL75311.1"
                     /translation="MRIKPAPGRQVPDPERGGYLSAEGRTVEPTQYWLRRLFDGDVIE
                     VQPSAKVRKGAK"
     gene            5696..6139
                     /locus_tag="VM406_04795"
     CDS             5696..6139
                     /locus_tag="VM406_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75312.1"
                     /translation="MQFNNIPLNVRVPLFYAEVDNSQAGYFSQTLRTLIIGQMLATGV
                     AAANVPQLVSRTDAAKEQFGVGSMLAAMHAKYRANDSFGEVWCLPLADGAGAAATGSF
                     AISGTASKAGTLSAYIGGERIQVAVAAADAAAAAATATWMRSPPM"
BASE COUNT         1211 a         1917 c         2004 g         1009 t
ORIGIN      
        1 ctgctgcaca gtgcgccgaa cgaccagatg acggcgttcg agtacaagga aatgcagcag
       61 gcgcacatgg gcctgcgcgg taacgcctat tcgttcatcg agagggatgg tcgcggtcgc
      121 atccagtcgc tcctgccgca ggatccggac aaggtgacgg tgcgacgcaa tcccagcgac
      181 ggcgctatcg tctacgacgt gtccggcgtc gaaaacggca ttccggcacg tcgcatgttc
      241 cacccacgcg gcttctcact cgacggactg atcggtctga acccgatcgc gctcgcgcgc
      301 gaaacgatcg gcctggcgat cgccggcgag aaggccggtg ccgaggcggt gggacgcggg
      361 atcgtgccgc cagcggcgct ggaggtgccg ggcaccccgg acaagaaaca gcgtgccgac
      421 atccgggagt cctggcgcga gatccaccag gggaacaaga acgacatcgc cgtcatcggc
      481 ggcaacatga agctgcacga tctgcgcatc aacatggcgg acatgcagtt tatggagtcg
      541 cgcaagttcc aggtgacaga gatcgccagg atgttccgga ttccgccgca catgctggcc
      601 gatttggagc gcgccacctt cagcaacatc gagcaccagg atctgcagtt cgtgaagtac
      661 acgctgctgc cctggctggt tcgctgggag cagcgcgcga acctgtcgct cctgtcggcc
      721 gacgagcggg aggaaggcta ctacgtgaag ttcaacgcag cggcgctgct gcgcggcgac
      781 atgaaggcgc gcttcgagtc ctacaagatg gggcgtgaca tgggcgtcta caacgcgaac
      841 gagatccgtg gtctggagga tctggatccg tacgaaggcg gcgacactta tctggaaccg
      901 ctcaacatgc aacgcgtcgg cgagccgcgc gaagaggagc aggaatgaaa aaggcttacc
      961 ttgccgagct ggtgttcaac acccctctgg cgatccacgg cgcgaagctg gaagtgatcc
     1021 tgaatgtcct gaagggacat ctgaacattg aggatattgg cgcgttcgat gcctccagtc
     1081 tgcctgctgg cttgaacgcg gcgaagcaag gagagaccac ggcctacgat cttggccatg
     1141 gggtggcggt tctgccggtg cacggatcgc ttacgcaccg cactcgcggc ctggatgcaa
     1201 tgtccggtct gcacagctac aagcagatgt ccatggactt ccgagcgatg ctgaacgaca
     1261 gctcggtgcg ccacatcgtc ctcgacgtga actctccggg tggccacgtg aatggcctct
     1321 tcgatctcgc tgatgaaatt ttcgctgcgc gcggaacgaa gccgatttcc gccatcgtgg
     1381 acgagtcggg ctacagcgcg gggtatgcca tcgcgtcggc agcggacgaa atcatcgttc
     1441 cgcggatggg cggggttggt tcggtcggaa tcgttgccgc gcatgtcgat aagagcgcgg
     1501 caatggagcg tgagggcgtg aaggtgatcc cgatctacgc cggtgcgaga aaggccgatg
     1561 ggctccctgg cgtgccgttg accgatgagg cgcatgcccg gctgcaggca gaggtcgacc
     1621 tggcgtatga gttgttcgtg gaaaccgttg cccgcaaccg cggcatgtct gtcgaggctg
     1681 tgaaagcgac cgaggcgggc acctaccgtg gtgcggatgc agtacgggtt cggttcgcgg
     1741 acagggtgat gaccgcgaac gatgcgatcc gcgagatcgt tgcgaagaat gcaggcggct
     1801 cggccgcgcg caactcgaac gggcgcgtcc agcgcgccgc ccaggcgatg cgtattcgct
     1861 ccgcttaaac caagcatcat caggatcccc gcgaggggta agttgccgcc cgctgggcgg
     1921 ctttttcatt ttgaaaggaa agcaatgaaa gacatcaaca gtcttctgca gcgccgcgcg
     1981 ggggtgcaag cacgtgtcgc ggaactggcc gcgctcgagt ccgcaggcac tgaactgacg
     2041 gcagagcagc tggccgaatt cggcgccctg caggccgagt tcgacgacct gagcgccaaa
     2101 atcggccgca tccagtcggc agagcgcatg gccgctgtga ccgccacccc gctgaccggc
     2161 ctgaccgttc cggccgtgcc gaaagccgca aagcagcagg gcgacgacat cggcatgatc
     2221 gtgcgctcgc tgtgcgcctc gaagggcgat ccgcgcgccg cggcgaactt cgctgacacg
     2281 cacggctatc ccgagctggc agccgcactc aataccggca ccaactctgc gggtggcttc
     2341 atcgtaccgc cgggctacgt gccggagttc atcgagatgc tgcgtccgcg ctcggtcgtc
     2401 cgtgcctctg gcgcgcgcgt ggtaccgatg ccgaacggca cgctcaccat gccgaagctg
     2461 accggcagct cgaccgccac ctaccagggt gagaacgacg acatcacccc gtctcagcct
     2521 gccttcggtc agatgaccct gactcgcaaa aagctgaccg cactcgtgcc ggtttcgaac
     2581 gacctgatcc gcttttcgag cccgcaggcc aacacaatcg tgcgcgacga catgctgcag
     2641 tcgatcggcc tgcgcgaaga catcggcttc ctgcgcgacg acggcaccgg caacctgccg
     2701 aaaggcttgc gccactgggc gccggccggc aacgtgatcg cggccaacgc cacgatcaac
     2761 ctgcagaacg tcaagaacga tctgggcaag ctggagctcg cgctgctgag caacaacgtg
     2821 cggatgatcg cgccgggctg ggtgttcacc cctcgcacga tggtgttcct gcagaacctg
     2881 ctggacggga atggcaacaa ggcctttccc gaaatcgacg ccggcatgct gcgcgggaag
     2941 ccgtacaagc tgaccaccca gatcccggaa aacctcggtg caggcagcaa cgagtcggag
     3001 atctacctgg tcgacttcaa cgaagtcatc atcggcgaag ccaccgggct gatcatcgat
     3061 gtctccagcg aggcgtcgtt caaggacggc gcgaacatgg tctccgcgtt ctcgcaggac
     3121 ctgacgctgg tgcgcgcgat caccgagcac gacatccagc cgcgccacaa cgctgcagtg
     3181 gcggtgctga ccgccgtccg ctggacgccg taatcagctg ctgacggatg gggatggctg
     3241 tgcgccatcc ccctatgtgc agacgtctgc accattcctc ttcacctgga gttgtcatga
     3301 aaaaggtcgt catcaagttc accgcaacgg tgagcccgta tcagaccggc gagatcgccg
     3361 gcttcgacga agaaaccgcc aagaagtaca tcgcagccaa gctggccgtc gagtacaagg
     3421 ccgacaagaa gtccgccgac gacggcgccg gagacggcag ccagaaatag ccgtcctgat
     3481 agggcgggcc ggtcaagccg tgagtgccgg cgtagtgaaa aaccacggta aggccccgcg
     3541 ccgctgcata ttctccagcg tgagctgggg ccccgattcc agacatgagt gagcaccatg
     3601 aaccttcgtc ttgtgacgcc gcccgatggc gagctgattc cgctgaacga ggtcaagact
     3661 caccttcgtg tcgattccgc attgaccgcc gatgacgggc tgatccagtc gctcgtgacg
     3721 gcggccatgc agaccgccga gcaccggacc ggccgctccc tgctgccgca gacgtgggag
     3781 ctggcgcttg atcgcttccc ggaaaaaatc gctctcgaca agccgccaat catcgaggtc
     3841 gaaagcgtga agtatgtgga cgccaatggc gacctgcaga tccttcctga gagcgactat
     3901 ctactggacg acttcggcga gcccgggcgc ctgattcccg cctacagcaa gtcatggcca
     3961 atgacacgcg aacagccgaa tgcggtgagg atccggttcc gggctggcta cgagaacgcc
     4021 gccgcggtcc cgcaagcgat caagcagtgg atcctgctga ccgtggggac gatgtacgag
     4081 aaccgtgaag gtgtggtcgt gggtcagtcc gtccagtcgc tgccgcatat cgacggcttg
     4141 ctggaccggg ccactgtttg gaggtgatat ggcggcaggg cgtctgaaca agcggctccg
     4201 gatccgcgcc tggcaggaca acccgagcgg aatggcagac atcgagccga catattccgc
     4261 cggcatggaa gtatgggccg gcgtcgagcc gacacgcggc tccgtctact acggcgcgaa
     4321 gcagaccggc gacgatgtga cgcacctgat caccatgcgg ctctcaccga ctgtgaacga
     4381 gcggacagta accccgagtc acgtgatcga cgatgaggtg gcgggcactc gttaccgcgt
     4441 caagcgatcg atggacctca aagggcaggg gcgctggctc cagatcgagg cggaattgct
     4501 gggagcaatc tgatgcttga ggtcaacgtc aaactcgccg gccacacggc tctcgacttc
     4561 gacaagaaga agatgcgccg cgccctgcgg caaatcggtg gaggtgtgcg caaggaagcg
     4621 cggcgcctgg tggcgcggcg tgccgtctcg cagcccggac aggatcccgg ccgcgacagc
     4681 ggcacgttat cgcgcagcat caaggtcaag gttttctcgt ccggcatggg cgctgttgtc
     4741 tcgccgcaga agactgccga gatgggagac gcgttctatc cggcgttcct ggtctacggc
     4801 gtgcggcgcg acgccaagcg ccgcaaggac caccagcggc aggtcgacaa cggcaagggc
     4861 tggcgtatgg cgccacgtaa cgatttcatg atgtccgcgc agttcaagcg cagcgacgtc
     4921 agcaaggcag ctttgatgga ggcgctgaaa gacgcgctca agccacgatg aacctgaaca
     4981 tcgtcattgc acatctgcgc gcgcgagtgc cggagttcgc caaccgcgtg gccggtgctg
     5041 cgcagttctc cgggatcccg gagagcgcca acctgccggt gccggcggcc tacgtgattc
     5101 cgatggacga gagcccggag aacaacgtta gccagaacgg ctatcggcag cgggtacgcg
     5161 agggcttcgc cgtcgttgtc gtgctctcca acacgccgga cgagcgcggc caggcggcgg
     5221 cgctgaccgt gcacgacatg cgggccaagc tgttccgggc gctgctcggc ttcgaggtcg
     5281 gtgaggagta cggcggcatc gaattcgacg gcggcaatct gctgcgcatg gaccgcgcgc
     5341 gaatgtacta ccagttcgag ttcgccgccg actacgagat cggcgaggaa gacacctggt
     5401 tggatgtgcg cgacagcgac ctgcccgact gggagggtgt cgacatcgat gtcgatctct
     5461 cgatgccgga cggccagatc gacgcaacgc tctccatcga ccttcctcac cagtaggagc
     5521 aactcatgcg aatcaaacca gcgcctggtc ggcaagtgcc cgacccggag cggggcggct
     5581 atttgtccgc cgaaggccgc accgtcgagc ccacccagta ttggctgcgt cgcctgttcg
     5641 atggcgacgt gatcgaagtt caaccctccg caaaagtccg taaaggagcg aagtaatgca
     5701 attcaacaat atccctctga acgttcgcgt cccgctgttc tatgcggagg tcgacaattc
     5761 gcaggccggc tacttctcgc agaccctgcg cacgctgatc atcggccaga tgctggctac
     5821 gggcgtcgcc gctgccaacg tgccgcagct ggtgagccgc acggacgccg ccaaggaaca
     5881 gttcggcgtc gggtcgatgc tggccgccat gcacgccaag taccgcgcca acgacagctt
     5941 cggcgaagtg tggtgcctgc cgctggctga tggcgccggc gcggcggcga ccggttcgtt
     6001 cgcgatctcc ggtaccgcat ccaaggccgg cacgctcagc gcctacatcg gcggcgagcg
     6061 catccaggtg gcagtggcag cggcggacgc tgcggccgcc gctgccactg ccacctggat
     6121 gcgctcgccg ccgatgtagg c
//
LOCUS       DATLGF010000159         3530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_178088, whole genome shotgun sequence.
ACCESSION   DATLGF010000159 DATLGF010000000
VERSION     DATLGF010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178088"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VM406_04800"
     CDS             complement(<1..368)
                     /locus_tag="VM406_04800"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVL75313.1"
                     /translation="MNQTAKKVLVVGASGGSGRAAVEALLAQGCEVTAFSRHADSLAS
                     MPGRLRTLRGDALDPAQVDAVVAGQDAVIVTLGISEPALRVRLFGSAGTAINVRSAGT
                     RNVIAAMHRHGVRRLVVQTS"
     gene            503..1471
                     /locus_tag="VM406_04805"
     CDS             503..1471
                     /locus_tag="VM406_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007739025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVL75314.1"
                     /translation="MNDKTPWHSVDPLGEALHFLRMSGVFYCRSEFSAPWGLALPSMP
                     DCLMFHVVNSGRCWLQATEEPALLLQPGDFVLVAHGAGHRLLSEPHAKAQDLFTLPRE
                     EVSDRFEILRHGGGGAGTQLICGAVRFDHPAARRLVALLPPVLAIDGGSLADAGWMQA
                     TLRLMAAEAGSLRPGGEAVITRLADILVIQAIRSWLAKAPGAQSGWLGALQDRQIGRA
                     LALIHREPGREWTLAGLAGEVAMSRSAFAARFAELVGVPSMQYLAHWRMQLAQGWLQE
                     SDAGVAELAARLGYRSEAAFCRAFKRVTGMAPGVARRGGEDAGVDR"
     gene            complement(1684..2112)
                     /locus_tag="VM406_04810"
     CDS             complement(1684..2112)
                     /locus_tag="VM406_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL75315.1"
                     /translation="MITLIERLDAYQSALYPAESNHFVDIAALARPNVLFAVARDGTG
                     SAVGCGAIVLEADHGEIKRMFVCESWRGRGLSRRLLSFLEHAARERGCTSLRLETGIH
                     QPEALGLYARAGFMRRGPFGSYREDPLSVFMEKNLEADRQ"
     gene            2263..3225
                     /locus_tag="VM406_04815"
     CDS             2263..3225
                     /locus_tag="VM406_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVL75316.1"
                     /translation="MKMRALLAGMVLSLWTSVAMADAWPAKTIRFVVAAPAGSSLDVI
                     ARTLSEKLKDRLGQSFVVENIPGASGTIATGQVARATPDGHTILISFNGPLAFTQFLT
                     KLNYDPQRDLAPVILTSSQPNVLAVNANVPVNNVRELIDHLKKNPGRMNYASVGNGSS
                     SHLTAELFKSETGTFIVHIPYNGAPPAALSLASGDTQLLFTIPTVINPQVKAGRVKAL
                     AVTSAKRFPLLPELPTVAESGLPGFESIAWNGILVPAGTPPEVIERLNREINAALQLP
                     DVRTRLSNAGLEVAGGSADAFRSLMASEAKKWSEIIAKTGAKLD"
BASE COUNT          583 a         1160 c         1166 g          621 t
ORIGIN      
        1 aagctggtct gcacgacgag gcggcgcacg ccgtgcctgt gcattgcggc gatcacgttg
       61 cgcgtgccgg ccgagcggac gttgattgcc gttcccgcgc tgccgaacag gcgcacgcgc
      121 agcgccggct cgctgatgcc gagggtcacg atgactgcgt cctgcccggc gaccaccgcg
      181 tcgacctgcg cgggatcgag cgcgtcgccg cgcagtgtgc gcagccgccc cggcatcgac
      241 gcgagcgagt cggcgtggcg ggagaaggcg gtgacttcgc aaccctgcgc gagcagggct
      301 tccaccgcgg cacggccgga gccgccgctc gcacctacca ccaggacttt cttcgctgtc
      361 tgattcatgt cgacctccat cgcttgttgg tgggtcaatg tagggcggca aactgcgacg
      421 aacaatgcgc accagcccgc tcttcttgcc ctttcgtcca gcgatgctgg acggctgatg
      481 catggcgtcc gatactgccc ccatgaacga caagactccc tggcacagcg tcgatccgct
      541 cggcgaggcg ctgcatttcc tgcgcatgag cggcgtgttc tattgccgtt cggaattcag
      601 cgcgccgtgg ggactggcgc tgccgtccat gccggattgc ctgatgttcc atgttgtcaa
      661 tagcggccgc tgctggctgc aggcaacgga agagccggcg ctgctgctgc agccgggcga
      721 tttcgtgctg gtcgcgcacg gcgccggcca tcgcctgcta tcggagccgc acgcgaaagc
      781 gcaggacctt ttcacgctgc cgcgcgaaga agtgagtgac cgcttcgaga tcctccgcca
      841 cgggggcggc ggcgccggga cgcagctgat ctgcggtgcg gtccgcttcg atcatccggc
      901 ggcgcgccgg ctggtggccc tgctgcctcc ggtgcttgcg atcgatggcg gcagcctcgc
      961 ggatgccggc tggatgcagg cgacgctgcg cctgatggcc gccgaggccg gcagcctgcg
     1021 ccccggcggc gaggcggtga tcacgcgact ggcggacatc ctcgtcatcc aggcgatccg
     1081 cagctggctc gcgaaggcgc cgggggcgca gtcggggtgg ctgggcgcat tgcaggacag
     1141 gcagatcggg cgcgcgcttg cgctgatcca ccgcgagccg ggccgcgagt ggacgctggc
     1201 cggactcgcg ggcgaggtcg cgatgtctcg ctcggcgttt gccgcgcgct ttgccgaact
     1261 ggtcggcgtg ccttcgatgc agtatctggc gcactggcgc atgcagcttg cgcagggctg
     1321 gctgcaggag agcgatgcgg gcgtcgccga actggcggcg cgcctcggct atcgatcgga
     1381 agcggcgttc tgccgggcgt tcaagcgtgt gactggaatg gcgccggggg tcgcgcggcg
     1441 cggcggggaa gacgctggtg ttgaccgctg acggcgggcg gcgcgcagat caccggcaga
     1501 taaactcgca gcgtgggccg ctgcgtccca cccagcgccc gtccgggcac tgcttcgcat
     1561 cctgcgtgca catgatctgt tcctcgcgcg gcacgtttac ggggtctgca tcggggcgcg
     1621 gcattgttgc cggcacgcac gacaccagca tcaagccgca taacgcggat aaaacttttt
     1681 ccttcattgc ctgtctgcct ccaggttctt ctccatgaac acggaaagtg ggtcctcgcg
     1741 gtaacttccg aacgggccgc ggcgcatgaa gccggcgcgc gcgtagagtc ccagcgcctc
     1801 aggctggtgg atgccggtct cgagccgcag cgaggtgcat ccgcgttccc gtgccgcatg
     1861 ctcgagaaac gacaacaggc gccgtgacag tccgcgcccg cgccaggatt cgcagacgaa
     1921 catgcgcttg atttctccat gatccgcttc cagcacgatt gcgccgcagc cgacggcgct
     1981 gccggtgccg tcgcgggcga cggcgaacag cacgttgggt cgggcgagcg cggcgatgtc
     2041 gacgaagtga ttgctttctg ccgggtacag ggcggattgg taggcatcga gccgttcgat
     2101 cagggtaatc acgtcgggct gatttggaga ttcgcaggcg atttgcacgg tctggatcat
     2161 tccatgaaga aagagaattg agatgtatca atggggccgg tacctgaaaa cggtccggaa
     2221 gtgacacata atggcccact tttccccctt ggagctatcc cgatgaaaat gcgcgccctg
     2281 ctggccggca tggtattgag cctttggacc agtgttgcga tggccgacgc ctggcccgcc
     2341 aagacgatcc gattcgtcgt tgccgccccg gccggctcgt cactggatgt gatcgcgcgc
     2401 acgctgtcgg agaaattgaa ggaccggctg ggccagtcct ttgtcgtgga aaacatcccc
     2461 ggcgcctcgg gcacgatcgc aaccggccag gtcgcgcgcg ccacgccgga cggccacacg
     2521 atcctcattt ccttcaacgg cccgctcgcc ttcacccagt tcctgaccaa actgaactac
     2581 gatccgcagc gcgatctcgc cccggtgatc ctgaccagct cccaaccgaa cgtgctcgcc
     2641 gtcaacgcca atgtgccggt caacaatgtg cgcgagctga tcgaccatct gaagaagaac
     2701 ccgggccgga tgaactatgc gtctgtcggc aacggctcgt cctcgcacct gacggccgaa
     2761 ctgttcaaga gcgagaccgg caccttcatc gttcacattc cctacaacgg cgcgccgccg
     2821 gccgcgctgt cgctggcctc cggcgatacc cagttgctgt ttacgatccc gacagtgatc
     2881 aacccccagg tgaaggccgg ccgggtgaag gcgctggcgg tgaccagcgc aaagcgcttc
     2941 ccgctgctgc ccgagttgcc gacggtcgcc gaatccggcc tgcccggctt cgaatcgatc
     3001 gcctggaacg ggattctcgt tcccgccgga acgccgccgg aagtgatcga acggctcaac
     3061 cgcgaaatca acgccgcgct gcaactgcct gatgtgcgga cacggctcag caatgccggc
     3121 ctggaagtgg ccggcggcag cgccgatgcg ttccgttcgc tgatggcctc ggaagcgaag
     3181 aagtggtccg aaatcatcgc gaagaccgga gcgaagctgg attgactgcg ggaaggggat
     3241 ttgccggacc taccgttcac ccgggcgacc atgcttctca ttgttgctcc cttgggcgag
     3301 cccccgccca ggggagcgac agagcaggca tagccgttca agcgaatgac gtttccatcc
     3361 cgcccgccct tctccatcac ctgcgatagc aaccatgaga ttcaacacgc aacttgccca
     3421 gctgggtcgc gcgagcgcaa ctgccgccca ggccgtgaat ccgcctctgg tccgtgcatc
     3481 caccacgctg ttttcctcgc tggcggaata taagaaatcc tatgacgggg
//
LOCUS       DATLGF010000160        11759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_3391,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000160 DATLGF010000000
VERSION     DATLGF010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 11759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11759
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3391"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VM406_04820"
     CDS             <1..362
                     /locus_tag="VM406_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75317.1"
                     /translation="VEQMGTQAASTQSEATRHAGKAAKTTQTQASTAAGAAADTMYNP
                     MMSMFSLWESAFREASAMATRGMSAAQSGFDRTTQTASGFAESMMHAAEDVTHTTAHA
                     GRKAASEAADGSSSRKR"
     gene            complement(374..1705)
                     /locus_tag="VM406_04825"
     CDS             complement(374..1705)
                     /locus_tag="VM406_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HVL75318.1"
                     /translation="MDEQALVGSIAARAVPLSGALDVDAVLKDIGDARIVLLGEATHG
                     THEFYRCRAEISKRLIAEREFDAIAIEGDWPDALQIDREVRQHADHDDSLLAARQALT
                     AFHRFPQWMWRNREMLELCAWLHRHNRERKNPQQRAGVYGLDLYSLRRSMEAVVAYLS
                     RVDPAAAREARARYGCFDQVADDPQRYGYRASFGMNEDCEDEVVQQLLAMRARDHSDV
                     QGDEEAFFAERNAAVAQNAEAYYRAMFHGRNHSWNLRDTHMAETLQALAEHIEKRKGR
                     PAGLIVWAHNSHVGDARATEMGEEGQLTLGQLVRERFSAKCYLLGFTTHAGTVCAASD
                     WGGRFEHKAVRQSRRDSFENLFHRCAPEDFFLSLRGWKQAGQPSLGRRLERAIGVIYL
                     PEHERASHYFHADITAQFDGVIHLDTTRAVTPLDPEGIPAPGPADTFPTGE"
     gene            1815..2252
                     /locus_tag="VM406_04830"
     CDS             1815..2252
                     /locus_tag="VM406_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVL75319.1"
                     /translation="MQKISDVMTRDVRTISPHDSIKRAAQLMDELNVGALPVCEGQRL
                     VGMITDRDITVRATSAGESPEMVSVEEVMTDQVRWCTEDQTVDEVMQQMADVQIRRIP
                     VLDRDTHQLIGIVSLGDLATKHSAQVDRTLEGISTPAQPDRPQ"
     gene            complement(2327..2980)
                     /locus_tag="VM406_04835"
     CDS             complement(2327..2980)
                     /locus_tag="VM406_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75320.1"
                     /translation="MVLAANQVLFNGNGIGYEAFSTSGAYGQAPGTNAPLSSFGLRVY
                     QEGMAGQSNTVRVALEMDQTDGSALLQMLVDRATLSISETGALSITVPDNANAYVVVN
                     DGAGNSATVSTSEIADNVIRFAPIADDPTSFGLIVDVDAIATAAAAASPQAATALQTL
                     RTLAARYDMRMAITGVGLRSSGGNDLTAGTTPITVGDQPAVSGGGVAGVFLGGGLTP"
     gene            complement(3003..3131)
                     /locus_tag="VM406_04840"
     CDS             complement(3003..3131)
                     /locus_tag="VM406_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75321.1"
                     /translation="MLKLKNAALTLAVVMALAACGGVPGLFGSEHAGSEQPGHAAG"
     gene            3713..4486
                     /locus_tag="VM406_04845"
     CDS             3713..4486
                     /locus_tag="VM406_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HVL75322.1"
                     /translation="MKLLSPSLLVCAALALSACGGSGGSGDGSPLQPGNPNPPGQNPP
                     PQEPPSQPPPPNTDPYVIEYHGDSTIWGWETGTSGARVGTPAPTAFANALPSTPQNEV
                     RNLGINGSTACGLLEGANGQETWATKMAESNATHVILNHGINDSSNGAVGYDVPRYRS
                     CLTQLAAIARANGKEVVFETPNPIAGSRMDDYVVAMRSVAATQQPAIPVIDQHQYLTD
                     FLAGRDVHEIVPDGAHPSQQTYIMKGEYAAQRFMAIFPR"
     gene            4630..5742
                     /locus_tag="VM406_04850"
     CDS             4630..5742
                     /locus_tag="VM406_04850"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HVL75323.1"
                     /translation="MIRSLEGMRGIAALIVALYHLGIGAQHVAVIRNGYLFVDLFFVL
                     SGFIMCAAYGRRLTGGEDLRVFLIRRIGRLFPLLVFSTLAFILAANAVVFAKRLLLER
                     GYGAMLHNPDGLQWVIPTASEILATVTLTHSMGVFDDLILNTPSWSISVEFWAYVLFA
                     LLCLGLTGRNRLMMFGAVSVVAAVITVWASVTVHQCLVQRGCLSLTYDFGFPRAVFSF
                     FLGALAFYASRHVRGMHGMLQAASLVLLAVVFLVVDHAPGIAFILPLVFALLILSVCS
                     DEGPMSSLLRPRFFQLLGLWSYSIYLMHMPLLLFFENLSRRFDGLFGGIAVMASFVAV
                     LLAVSAWTYRYVEDPMRAWFNRMAVRREVPAGSTNL"
     gene            5745..6407
                     /locus_tag="VM406_04855"
     CDS             5745..6407
                     /locus_tag="VM406_04855"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HVL75324.1"
                     /translation="MRLSAILLCCALAAATASQAQPRPVLVEYYGDSTVWGYASGSDG
                     RQVARPAPQVFAEALRTRVRVDVRNHGVSGSTACALLEGTDGRHPPWPEQMRASKAQF
                     VILNHAINDQWRHDLEAYRACLRELARTARAHGKQVIFETPNPTRDSGVGGLDTYVAA
                     MKEVALQEGLPVIDQYTFLNDKLRGRSPLLFAPDGLHPSDTVYVMKGMFAAHAFSRLF
                     GL"
     gene            6622..7092
                     /locus_tag="VM406_04860"
     CDS             6622..7092
                     /locus_tag="VM406_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVL75325.1"
                     /translation="MYKHILLPTDGSALAREAVQSGILFAKNIGAEVLGLYVIPEPRA
                     EQLDAWLHHDPLFPKHRKMMFDKFADEALSFVANSALAEEVPCLYRKVQGAEPWIAIL
                     KVAEQSHCDLIYMASHGWKGGIGDHVGSQTLKILHHSPIPVLVHKPRKAATAPA"
     gene            7171..8169
                     /locus_tag="VM406_04865"
     CDS             7171..8169
                     /locus_tag="VM406_04865"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HVL75326.1"
                     /translation="MRVAVFSSKHYDRIFLDEANGGRHELVYHEARLTPATAALAEGF
                     AAVCAFVNDQLSEPALEMLHAGGTRLLALRSAGFNHVDLAAAERLGMTVARVPAYSPH
                     AVAEHAAALVLSLNRRTYRAYNRVREGNFSLDGLLGFDLNGKTVGVIGTGKIGLVFAR
                     IMHGFGCRILASDPTPAADAPGHVEYVDTDTLYRESDIISLHCPLTPQTRHLIDGAAI
                     AQMKRGVMLINTSRGQVVDTAALLAGLKSGRIGFVGLDVYEEEESLFFQDLSDRVIAD
                     DMFMRLTTFHNVLITGHQGFFTREAMGNIAAITIGNITAFESGQGTLHRVGAQRLA"
     gene            8166..9698
                     /locus_tag="VM406_04870"
     CDS             8166..9698
                     /locus_tag="VM406_04870"
                     /inference="COORDINATES: protein motif:HMM:NF014683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HVL75327.1"
                     /translation="MSALAALPIAVILVLMIGLGWSAARAGALGLVAALVIAWAAFGL
                     GARVMPELGAAAAAGGALGEAVFLALTILWIIFPALCIHELQVRTGSIDVLRVAMTRI
                     AGDARILALLVAWFFGLFIEGAAGFGTSVALAAPFLVSAGFGRVAAVSMALIGHAIGV
                     SFGAVGTPVVPQAAVSGLSPLAIAGATANYHLLIGWMMPLAVMFLATRAQAATPASRV
                     APAPWGWTFVAAACFLVPYYLIARLIGPELPTLAGALVGGLLFVGALRMARRRSGAEA
                     DATRAADLLRAAAPYLLLIALVVCTRMIGPLQERLRAVEIAWTYASFGGSMQILYHPG
                     TMLLASFLLGALVQRVPAVTLREAIGAVLRRLLPVTVALAAMLGLSRVMVHAGMIDAL
                     AAAAAAAAAGTWPALWPVLAPAIGLLGTFVTGSATASNILFTEFQVATAERLGLSVLG
                     LTAAQGFGAAIGNIICPHNIVAASATVQLVGREGEVLRRTLLPALACALAGGFLALWL
                     YA"
     gene            9887..10333
                     /locus_tag="VM406_04875"
     CDS             9887..10333
                     /locus_tag="VM406_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HVL75328.1"
                     /translation="MAGNLMRFDPMRELTRFDPFRGMDDMLKEFSLAPSLRGLEPEPR
                     IRLDITESDDAYEVKADVPGVKKEDIKISIEGGTVSIRASAREESERKEGNMMCSERY
                     YGEQYRSFTLPAEVDDSKAEARYQDGVLHLKLPKKEGRTAKQITVQ"
     gene            10347..>11759
                     /gene="ppsA"
                     /locus_tag="VM406_04880"
     CDS             10347..>11759
                     /gene="ppsA"
                     /locus_tag="VM406_04880"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HVL75329.1"
                     /translation="MDAPGAQFIRWFADLGVADVAVAGGKNASLGEMVRALAPRGINV
                     PNGFAITADAYRYLLDSAGAWDALRATLDGLNANDVTDLARRAHDAREIIYGAPLPDD
                     LAAQILDAYAALRRQYGELTLAVRSSATAEDLPTASFAGQHETFLNISGDARLLDAVK
                     RCFASLFQDRAIRYRIDNGFDHFKVLLSVGVMKMVRSDLAASGVMFSLDTETGFRDVV
                     FITGAYGLGENVVQGAVDPDEFYVFKPAFRNGARAVLRRSLGAKKIRMVYSGAAGRET
                     TRNVPTPREDRQRFCISDAEVLELAQAAIVIEEHYSGAAGTPMPMDIEWAKDGVDGKL
                     YIVQARPETVAAHRAPTMLEEYVLESKGQVRARGRAVGEKVVSGVARVISGVGQLHEF
                     RPGEVLVADTTTPDWGTVMKTASAVVTNRGGRTCHAAIVARELGIPAVVGCDNATERI
                     RSGDEVTVSCAEGDAGFVYAG"
BASE COUNT         1937 a         3859 c         3894 g         2069 t
ORIGIN      
        1 atgtcgagca gatgggcacg caggcagcga gtacccagtc cgaggcgacg cgccatgccg
       61 gcaaggcggc aaagaccaca cagacccagg ccagcaccgc ggcgggcgcc gcagcggaca
      121 ccatgtacaa cccgatgatg agcatgttct cgttatggga atcggcgttc cgcgaagcta
      181 gcgcaatggc cacgcgcggc atgtcggcgg cgcagtcggg cttcgaccgc acgacccaga
      241 ccgcctccgg gtttgcggaa agcatgatgc atgcggccga ggacgtcacg cataccaccg
      301 ctcacgccgg caggaaggcc gccagcgagg cagcggacgg ttcgagttcg cgcaagcgct
      361 agcgctagcg cgctcattcg ccggtcggaa acgtgtccgc cggccccgga gcgggaatgc
      421 cctcggggtc gagcggcgtg accgcgcgcg tcgtatccag gtgaatcacg ccgtcgaact
      481 gcgcggtgat gtccgcgtgg aagtagtggc tcgcgcgctc atgttcgggc aggtagatca
      541 cgccgatcgc gcgctccagc cgccggccca atgagggctg gcccgcctgt ttccagccgc
      601 gcagcgaaag gaagaaatct tccggcgcgc agcgatggaa cagattctcg aagctgtccc
      661 tgcgcgactg gcgcaccgcc ttgtgctcga agcgcccgcc ccagtcggag gccgcgcata
      721 ccgtccccgc atgcgtggta aagccgagca gatagcactt tgcgctaaag cgctcgcgca
      781 ccagctggcc cagcgtgagc tggccttcct cgcccatctc ggtggcgcgg gcgtcgccca
      841 catgcgagtt gtgcgcccac acgatgaggc cggccgggcg ccccttgcgc ttctcgatgt
      901 gctcggccag cgcctgcaag gtttcggcca tgtgggtgtc gcgcaggttc caggagtgat
      961 tgcggccatg gaacatcgcg cggtagtacg cctccgcgtt ctgcgcgacc gcggcgttgc
     1021 gctcggcgaa gaatgcttct tcatccccct gcacgtcgga atggtcgcgc gctcgcatcg
     1081 ccagcagctg ctgcacgacc tcatcctcgc aatcctcgtt catgccgaaa ctggcgcggt
     1141 agccgtagcg ctgcggatcg tcggcgacct ggtcgaagca gccgtagcgg gcccgcgcct
     1201 ctcgagcggc agccgggtcg acgcggctca ggtaggcgac gaccgcctcc atcgagcggc
     1261 gcaggctgta gaggtcgagc ccgtacacgc cggcgcgttg ctgcggattc ttccgttcgc
     1321 gattgtgccg atgcagccac gcgcacagtt ccagcatctc gcggttgcgc cacatccact
     1381 gcggaaagcg gtggaacgcg gtcagcgcct gccttgcggc gagcagcgag tcgtcgtgat
     1441 ccgcatgctg gcgcacctcc cggtcgatct gcagcgcgtc cggccagtcg ccctcaatgg
     1501 cgatagcgtc gaactcgcgc tcggcgatca ggcgtttgct gatttcggcg cggcagcggt
     1561 agaactcgtg ggttccgtgc gtcgcctcgc cgagcaggac gatgcgcgca tcgccgatgt
     1621 ctttcagaac ggcatccaca tcaagggccc cggatagcgg tacggcccgc gcggcaatgc
     1681 tgcccaccaa tgcctgctcg tccatcgttt gcacctccag gtcattggat ggcgatcgtg
     1741 ccggacggtt cgacatggca cttttactgt ctcttggctg tctgtgcgct tatctagtca
     1801 ccaaggagca ggcaatgcag aagatttcgg atgtgatgac gcgcgacgtg cgcacgatca
     1861 gtccgcacga cagtatcaag cgcgcggcgc agctgatgga tgaactcaat gttggcgcat
     1921 tgccggtctg cgaaggccag cgcctggtcg gcatgatcac cgaccgcgac atcacggtgc
     1981 gcgcgacgtc ggcgggggaa tcgccggaga tggtaagcgt ggaggaagtg atgacggacc
     2041 aggtgcgctg gtgcaccgag gaccagactg tggacgaggt gatgcagcag atggccgacg
     2101 tgcagatccg ccgcatcccg gtgctcgatc gcgacacgca ccagttgatc ggcatcgtct
     2161 cgctgggcga cctggccacc aagcattcag cccaggtcga ccgcacgctg gagggaatat
     2221 cgacgccggc gcagccggac cgcccgcagt aacgcggccg gaaaagaaat ggggcggccg
     2281 tacagcgccg ccccatcccc ccctcccgaa gagggttggc cgaagcttac ggggtcaggc
     2341 cgccgcccag gaagacgccg gccacgccgc cgcccgatac cgcgggctgg tcgcccacgg
     2401 tgatcggggt cgtgcccgcc gtcaagtcgt tgccgcccga cgaacgaagg ccgacgccgg
     2461 tgatcgccat gcgcatgtcg tagcgcgccg ccagcgtgcg cagcgtctgc agcgcggtag
     2521 ctgcctgagg cgatgccgct gcggctgcgg tcgcgatcgc gtccacgtca acgatcaggc
     2581 cgaagctggt cgggtcatcg gcaatcggtg cgaaacggat cacgttgtcc gcgatctcgc
     2641 tggtgctgac cgtagcggag ttgccagcgc catcgttcac caccacgtag gcattggcgt
     2701 tgtccggcac cgtgatcgag agcgcgccgg tctcgctgat gctcagcgtt gcgcggtcca
     2761 ccagcatctg cagcagcgcg ctgccatcgg tctgatccat ttccagcgcg acgcggacag
     2821 tgttcgactg gccggccatg ccttcctgat acacgcgcag gccaaaggag ctcagcggcg
     2881 cgttcgtgcc gggcgcctgg ccatatgcgc ccgaggtgct gaaggcctcg tagccgatgc
     2941 cgttgccatt gaacaggacc tggttcgcgg ccagtaccat gttgccctcg aaggtggcgt
     3001 cttcagccgg cggcgtgccc gggttgttcg gatccggcgt gttcggatcc gaacaacccg
     3061 ggcacgccgc cgcaggctgc gagcgccatg accactgcca acgtgagggc tgcgttcttg
     3121 agtttgagca tgtgaaatcc tctcttcggt ttaacgggaa agtggtattg cgggtagtac
     3181 acagccgacg ctacgtcgtt gctgagccgt cagattaggg cggggaaaca ggtacgcagt
     3241 tgcgggaagt caccgctgcc gacgcgcaca gttgcgacga gtcaagcgtc ggccatttct
     3301 acgcggaaga gcatttccac atgtaaagag aaggcggtgc ggcgagcaaa agggggccag
     3361 catcggcgct gatatgtcga tcactcgagc gactgtgctc gctatgtcgc ctccgggtag
     3421 gcgcggaccc cgcacaagcc tgtgcagccc aggctaaagg gggacctggg ctccgggcct
     3481 gagcgggggc gacattgcgc gagcatgaac gggaacccgc gccagtcttt cactctgcaa
     3541 gtccttacgg ccagttgcag ttttcgagaa tgcgcaacat ctaccggaag tgttcattaa
     3601 gcgatcacaa cgcacttgcg gcgcgccaat ggaatgatgt gtccagtctg catgcggcaa
     3661 aaaaacacga ccgcctgcgt ccgttatcac aacctttcat tggagaactc gattgaagct
     3721 gctctcccca agtcttctcg tatgcgccgc gctcgcattg agcgcgtgcg gcggatccgg
     3781 tggatccggc gatggtagtc cgctgcagcc tggcaatccc aatccccccg ggcagaaccc
     3841 gcctccgcag gagccgccca gccagccgcc gccgccgaac accgatcctt atgtcatcga
     3901 ataccacggc gactcgacca tctggggatg ggaaaccgga accagcggcg cgcgcgtggg
     3961 tacaccggcg cctacggcat ttgcaaacgc gttgccgagc acgccgcaga acgaggtgcg
     4021 caacctcggc ataaacggct ccaccgcctg cggcctgctc gagggtgcga acggccagga
     4081 aacctgggca acgaagatgg ctgaatccaa tgccacccat gtgatcctga accatggcat
     4141 caacgacagc agcaacggcg ccgttggcta tgacgtaccg cgctaccgct cctgcctgac
     4201 gcagctcgcc gccattgcgc gcgccaacgg caaagaggtg gtgttcgaaa ccccgaatcc
     4261 gatcgcgggc tcgagaatgg acgactatgt ggtagcgatg cgctcggtcg cagcaaccca
     4321 gcagccggcg attcccgtca tcgaccagca ccagtacctg accgactttc tcgccgggcg
     4381 cgacgtgcac gagatcgtgc cggacggtgc acatccgtcg cagcagacct acatcatgaa
     4441 gggcgagtac gcggcacaac gcttcatggc gatcttccca cgctgatccc gtccctgcgc
     4501 ctgccgtgct tttttttccg cacggcaggt gagcagcctc aagaagcggc accgcgccgc
     4561 gtcgcatact cgaagcaggc atgccgcccg gcatgtgaac tccgcaaccc agagaatgac
     4621 agccacttca tgattcgttc gctcgaaggc atgcgcggca tcgcggcgct catcgtcgcc
     4681 ctgtatcacc tgggcatcgg cgcgcagcac gtcgcggtga tccgtaacgg ttaccttttc
     4741 gtcgacctgt tcttcgtact cagcggcttc atcatgtgcg ccgcctacgg caggcgcctg
     4801 acaggcgggg aagacctgcg cgtattcctg atccgccgca tcggccgcct gtttccgctg
     4861 ctggtgttct ccacgctggc gttcatcctc gccgcaaacg ccgtcgtgtt cgccaagcgc
     4921 ctgctgctcg agcgcggcta cggcgcgatg ctgcacaatc ccgacggctt gcaatgggtg
     4981 atcccaacgg caagcgagat cctggcgaca gtcacgctca cgcacagcat gggcgtgttc
     5041 gacgacctga tcctgaacac gcccagctgg agcatcagcg tggagttctg ggcatacgtg
     5101 ctgttcgccc tgctgtgcct cgggctcacc gggcgcaacc gcctgatgat gttcggcgca
     5161 gtcagcgtgg tcgctgcagt cattaccgtc tgggcctcgg tcaccgtcca ccaatgcctt
     5221 gtgcagcgcg gctgcctgtc gctcacctac gacttcggct ttccccgcgc ggtattttca
     5281 ttcttcctcg gcgcgctcgc gttctacgcg agccggcatg tgcgcggcat gcacggcatg
     5341 cttcaggcgg cgtcgctggt cctgctcgcg gtggtgttcc tggtcgttga tcatgcaccg
     5401 ggcatcgcct tcatcctgcc gctggtgttc gcgctgctga tcctgtcggt atgcagcgac
     5461 gaagggccga tgtcgagcct gctgcgcccg cgattctttc agctgctcgg gctgtggtct
     5521 tactcgatct acctgatgca catgccgctg ctgctgttct tcgaaaacct gtcccgccgc
     5581 ttcgacggcc tgtttggcgg catcgcggtc atggcgtcgt tcgtcgccgt cctcctcgcc
     5641 gtgtcggcgt ggacttaccg ctacgtcgag gatccgatgc gagcgtggtt caaccgcatg
     5701 gccgtgcgcc gcgaggtgcc ggccggcagc accaacctgt gaacatgaga ctttccgcca
     5761 tcctgctttg ctgcgcgctg gcggccgcga ccgcgtcgca ggcccagccg cgacccgttc
     5821 tggtcgagta ttacggcgat tccaccgtct ggggctatgc gagcggcagc gacggtcgcc
     5881 aggtggccag gccggcgccc caggtgttcg ctgaagcgct gcgcacgcgc gtgagggtgg
     5941 acgtgcgcaa tcacggcgta agcggatcga ccgcctgcgc gctgctggaa ggcaccgatg
     6001 ggcgccatcc gccatggccg gagcagatgc gcgcatcgaa agcgcagttc gtgatcctca
     6061 accatgcgat caacgaccag tggcggcacg atctcgaggc ttaccgcgct tgcctgcgcg
     6121 agctcgcgcg cactgcgcgc gcgcatggca agcaggtcat cttcgaaaca ccgaatccga
     6181 cccgcgacag cggcgtcggc ggactggata cctatgtcgc ggcgatgaag gaggtggcgc
     6241 tgcaggaagg gctgccggtg atcgatcaat acaccttcct gaacgataag ttgcgcggcc
     6301 gcagtccgct gctgttcgcc cccgacggcc tgcatccttc cgacacggtc tacgtgatga
     6361 agggcatgtt cgcagcccac gccttcagca ggctgttcgg cctctgaatc cgattctcgt
     6421 cttcgattgc gcgccgcctt gagtacgctt gatccaggtc aaggccgccg aagtcgcggc
     6481 ggatgaagct tgggcgccaa atccgggatc acgcatcacg cgcggggaga gcgtcccctc
     6541 gccgagccgc atgcgcaggg gggcggtgtc gtgctgcaag cccggacagc catgctgttg
     6601 aagcaggaaa gggcgcgcgc catgtacaag cacatccttc ttccgaccga cggttccgcg
     6661 ctggcccgcg aagctgtgca gagcggcatc ctgtttgcga agaacatcgg agcggaggtg
     6721 ctgggactgt atgtgattcc cgagccgcgg gccgagcagc tcgacgcctg gctgcatcac
     6781 gatccgctgt ttccgaagca tcgcaagatg atgttcgaca agttcgcgga tgaagcgttg
     6841 agcttcgtcg ccaacagtgc gctggccgag gaggtgccat gcctctaccg gaaggtgcag
     6901 ggcgcagaac catggatcgc gatcctgaag gtggcggaac agtcgcactg cgatctgatc
     6961 tacatggcat cgcatggatg gaagggcggc attggcgacc atgtcggcag tcagaccctg
     7021 aagatccttc atcacagccc gatcccggta ctggtccaca agccgcggaa ggctgcgacg
     7081 gcgcctgcct gagcggcggc gattttccat gcctgcatga ccggttcccg ggaccggcag
     7141 cgggcttgtt cgtgtcatgg agtcagatgt atgcgcgtgg cggttttcag cagcaagcat
     7201 tacgaccgga tcttcctcga tgaagccaat gggggaaggc atgagctggt ctatcacgag
     7261 gcgcgtctga cgccggccac cgcggcgctc gccgagggct ttgcggcagt ctgcgcgttc
     7321 gtcaacgacc agctatccga gccggcgctc gagatgctcc acgcgggcgg cacgcgcctg
     7381 cttgcgctgc gctcggccgg tttcaaccat gtggacctgg cggcggccga gcgcctcggc
     7441 atgacggtgg ctcgggttcc ggcctattcg ccgcacgccg tcgccgagca cgcggcggcc
     7501 ctggtgctga gcctgaaccg gcgcacctac cgcgcctaca accgcgtccg cgaaggtaat
     7561 ttttcactcg acggcctgct cggattcgac ctgaacggca agacggtcgg ggtcatcggc
     7621 accggcaaga tcggcctggt ctttgcgcgc atcatgcacg gcttcggctg ccgcatcctg
     7681 gccagcgatc ccaccccggc cgccgatgcg cccgggcatg tcgagtacgt cgacaccgat
     7741 accttgtacc gggagtcgga catcatctcg ctgcattgcc cgctgacgcc gcagacgcgc
     7801 cacctgatcg acggtgcggc gatcgcgcag atgaagcggg gcgtcatgct gatcaatacc
     7861 agccgcggcc aggtggtcga tacggcggcg ctgctggccg gcctgaagtc cggcaggatc
     7921 ggctttgtcg gcctggacgt ctacgaggaa gaggaaagcc tgtttttcca ggatctttcc
     7981 gaccgggtca tcgccgacga catgttcatg cgcctgacca cctttcacaa cgtgctcatc
     8041 accggacacc aggggttttt cacccgcgaa gccatgggca atatcgccgc gatcaccatc
     8101 ggcaacatca ccgcgttcga gagcgggcag gggacgcttc accgcgtcgg cgcgcagcgg
     8161 ctcgcgtgag tgcgctggcg gcactgccga tcgcagtcat cctggtcctg atgattggcc
     8221 tgggctggtc ggcggcgcgg gcgggcgcgc tcggccttgt cgcggcgctc gtcattgctt
     8281 gggccgcgtt cggactcggc gcgcgcgtca tgcccgaact cggcgcggcc gcggccgccg
     8341 gcggagcgct gggcgaagcg gtgttcctgg ccctgacgat actgtggatc atctttcccg
     8401 cgctgtgcat ccacgaactg caggtcagga ccggcagcat cgacgtgctg cgcgtcgcaa
     8461 tgacgcggat cgcgggcgac gcgcgcatcc tcgcgttgct ggtggcctgg ttcttcgggc
     8521 tgttcatcga gggcgccgcc ggattcggca cctccgtggc gcttgccgcg ccgttcctgg
     8581 tgagtgccgg cttcggccgc gttgcggcgg taagcatggc gctgatcggc catgcgatcg
     8641 gcgtttcctt cggtgccgtc ggcacgccgg tcgttccaca ggcagcggtc tcggggcttt
     8701 cgccgctggc cattgcgggc gccactgcca actaccatct gctgatcggc tggatgatgc
     8761 cgctcgcagt catgttcctg gccacacgcg cccaggctgc gacgcctgct tcacgcgtcg
     8821 cgcccgctcc atggggctgg acgttcgtgg ccgccgcctg tttcctggtg ccctactatc
     8881 tgatcgccag gctgatcgga ccggaattgc cgaccctggc gggcgcactg gtcggcggcc
     8941 tgctgttcgt cggcgcattg cgcatggcgc gacggcgatc cggtgccgag gcggacgcca
     9001 cgcgggctgc cgatctgctg cgcgccgctg ccccttacct gctgctgatc gcgctggtcg
     9061 tttgcacccg catgatcggt cccttgcagg agcgcctgcg ggctgtcgag atcgcgtgga
     9121 cctacgcatc gttcggcggc agcatgcaga tcctctacca cccaggcacg atgctgctcg
     9181 ccagcttcct gctcggtgcg ctggtgcagc gtgtgccggc ggtcacgctg cgcgaggcga
     9241 tcggcgccgt gctgcggcgt ctgctgccgg tgacggtggc gctggcggcg atgctcggcc
     9301 tgtcgcgcgt gatggtccat gccggcatga tcgacgcgct cgccgccgcg gcagcggctg
     9361 cggcggcggg gacgtggccg gcgctgtggc ccgtgttagc gccggcgatc ggcttgctcg
     9421 gcaccttcgt gaccggctcg gccaccgcgt ccaacatcct gtttaccgag ttccaggtgg
     9481 cgaccgccga gcgtctcggg ctgtcggtgc tgggcctgac ggccgcgcag ggtttcggcg
     9541 ccgcgatcgg caacatcatc tgcccgcaca acatcgtcgc ggccagcgcg accgtgcaac
     9601 tggtcggccg cgagggagaa gtgctgcggc ggacactgct gccggcgctc gcctgtgcgc
     9661 tcgccggcgg ttttctggcc ctctggctgt acgcgtaaga gcggccagca aggccagcaa
     9721 gccggcacga cgcgcaagcg taaatgtgcc ggcgcttccg acgcgccttc gcgcatcaag
     9781 cccgccttga tcttgatcat gcgtgagccc gttcggcttc ctaacatgaa ccgaccggga
     9841 gtggcgcatc gctccgcttc cgcttcaccg ttcacggagg caagacatgg ccggcaacct
     9901 gatgcgattc gatccgatgc gcgagctgac gcgcttcgac cctttccgcg gcatggatga
     9961 catgctgaaa gagttctctc ttgccccatc gctgcggggc cttgaacccg agccgcgcat
    10021 ccggctcgac atcaccgaga gcgacgatgc gtatgaagtg aaggccgacg tgccgggcgt
    10081 gaagaaggaa gacatcaaga tttcgattga aggcggcacc gtctcgatcc gggcgagcgc
    10141 gagggaagaa tccgagcgca aggaaggcaa catgatgtgc agcgagcgct actacggcga
    10201 gcagtaccgc agcttcacct tgccagccga ggtcgacgac agcaaggccg aagcgcgcta
    10261 ccaggatggc gtgctgcacc tgaagctgcc gaagaaggag ggcaggacgg ccaagcagat
    10321 cacggtgcaa tagcgaagcg cgcagcatgg acgcgcccgg cgctcaattc atccgctggt
    10381 tcgccgatct cggcgtcgcg gatgtggcgg ttgcgggcgg caagaacgcg tcgctcggcg
    10441 agatggtgcg agcgctcgcg ccgcgcggca tcaatgtgcc gaacggcttt gcgattaccg
    10501 ccgacgcgta ccgctacctt ctcgacagcg caggggcgtg ggacgcattg cgcgccaccc
    10561 tcgacgggct gaacgcgaat gatgtgacag atctcgcgcg ccgcgcgcac gacgcgcgcg
    10621 agatcatcta cggcgcgccg ttgccggacg acctcgctgc ccagattctc gatgcctatg
    10681 ccgcattgcg ccgccagtac ggggagctga cgcttgccgt gcgcagttcc gccaccgccg
    10741 aggaccttcc gacggcgagc ttcgccggcc agcatgaaac cttcctgaac atttcgggtg
    10801 acgcgcgcct gctcgatgcg gtcaagcgct gctttgccag cctgttccag gaccgcgcga
    10861 tccgctaccg gatcgacaac ggcttcgatc atttcaaggt cctgctgtcg gtcggcgtga
    10921 tgaagatggt gcgttccgat ctcgccgcct ccggcgtgat gttttcgctc gacaccgaga
    10981 ccggctttcg tgacgtggtg ttcatcaccg gggcctacgg cctcggcgag aacgtcgtgc
    11041 agggcgcggt cgatcccgac gagttctacg tgttcaagcc cgcgttcagg aacggcgcgc
    11101 gcgcggtgtt gcggcgcagc ctcggcgcca agaagatccg catggtgtac tcgggcgccg
    11161 ccggccgcga gaccacccgc aacgtgccga cgccgcgcga agacaggcaa cgtttctgca
    11221 tcagcgacgc cgaggtgctg gagctcgcgc aggcggcgat cgtgatcgag gaacactaca
    11281 gcggcgcggc cggcacgccg atgccgatgg atatcgaatg ggcgaaggat ggcgtcgacg
    11341 gcaagctgta catcgtgcag gcgcggccgg agacggttgc cgcgcaccgc gcgcccacga
    11401 tgctggaaga gtacgtgctg gagagcaagg gacaggtgcg agcgcgcggc cgcgcggtcg
    11461 gcgaaaaggt ggtcagcggt gtggcgcggg tgatttccgg cgtcggccag ctgcacgagt
    11521 tccggcccgg cgaagtgctg gtcgccgata ccacgacgcc ggactggggc acggtgatga
    11581 agaccgcttc agccgtcgtg accaatcgcg gcgggcgcac ctgccacgcg gccatcgtcg
    11641 cgcgcgaact cggcattccg gccgtggtcg gctgcgacaa cgccaccgag cggatccgct
    11701 ccggcgatga agtgacggtc tcctgcgcag aaggcgacgc cggcttcgtc tatgccggc
//
LOCUS       DATLGF010000161         2500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_147029, whole genome shotgun sequence.
ACCESSION   DATLGF010000161 DATLGF010000000
VERSION     DATLGF010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147029"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VM406_04885"
     CDS             <1..398
                     /locus_tag="VM406_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FCSD flavin-binding domain-containing protein"
                     /protein_id="HVL75330.1"
                     /translation="LANNRWCGVDYLTFESTVARDVHVLGDSIQIAPGMPKSGHMANN
                     HGKVAAAAIVAQLSGWAPNPAPMLTNTCYSFVDNRQVMHVASVHAYDAADRTFKTVPG
                     SGGVSTAHSEMEGVYAWNWARNIWADALA"
     gene            complement(493..1446)
                     /locus_tag="VM406_04890"
     CDS             complement(493..1446)
                     /locus_tag="VM406_04890"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HVL75331.1"
                     /translation="MKEQIPNETCWGVDVASEHLDIARHGSQQVQRIGNDPKAIAKWL
                     RSAPANSRLGLESTGSYHLQLARLAHERGITVYVLNPRDIKRYAQGLGQRGKTDRLDA
                     RVIARFIEREHEHLRAWQPPTQQQQELDDLLRERAALQKTYAALKQSVAKRPKPAAML
                     AKLLHEMKAALQDFERAIAQAVRELPQGDTAFGVITSIPGIGLLTGAALLRLFTRAPD
                     ASTDAIVALTGLDPRPMDSGTRRGVRRLSKRGDANTRRLLYNAGMAGAKTDTWRPIYE
                     RERAKGLSSTAALLVLARRLVRIAYSLFKSGQQFDRARVMT"
     gene            complement(1736..>2500)
                     /locus_tag="VM406_04895"
     CDS             complement(1736..>2500)
                     /locus_tag="VM406_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVL75332.1"
                     /translation="KGVLAGIAGDDGAMRRVIDGRPESIMRHCEDSLRRLQTDVIDLY
                     YLHRWDKKVPIEESVGAMSRLVEQGKVRAIGLSEVSAATLRKAHAVHPIAAVQTEYSL
                     WTRNPEIAVLAACRELGATFVAFSPVARGFLCDSLHDVGSLHEKDIRRGMPRFEPANY
                     AANLKLLPGYKALAQEAGCTPAQLALAWLLHKGEDIIPIPGTTSVEHLREDLAAVDVR
                     LSPDLMARLEAHINERTVSGNRYNQQSRSEVDTEEF"
BASE COUNT          381 a          851 c          813 g          455 t
ORIGIN      
        1 acctggccaa caaccgctgg tgcggcgtcg actacctcac cttcgagtcc accgtggcgc
       61 gcgacgtgca cgtgctgggc gactcgatcc agatcgcgcc gggcatgccc aagagcggcc
      121 acatggccaa caaccacggc aaggtcgcgg ccgcggccat cgtggcccag ctctcgggct
      181 gggcgcccaa cccggcgccc atgctgacca acacctgcta cagcttcgtc gacaaccggc
      241 aggtcatgca cgtggccagc gtgcatgcct acgacgcggc cgaccgcacc ttcaagaccg
      301 tccccggctc cggcggcgtg agcaccgcgc acagcgagat ggagggcgtg tacgcctgga
      361 actgggcacg caacatctgg gccgacgcgc tggcgtgagc gcgggctcca tcaacgcgga
      421 gcctgtcgaa ggcctcgcgc gggagccctt cgacaaacct cctccgttcg ggctgaggtt
      481 ctatggttct cgtcaagtca tcacacgcgc gcggtcgaac tgctgaccgc ttttgaacag
      541 gctgtaggcg atgcgcacca gacgcctggc caggaccagc aaggccgcgg tgctcgatag
      601 gcccttggcg cgctcgcgct catagatcgg tcgccaggtg tcggttttgg ccccggccat
      661 gccggcgttg taaagcagcc ggcgggtgtt cgcatcgccg cgcttggaca ggcgtcgaac
      721 acccctgcgg gtgcccgaat ccatgggccg tggatccaat ccggtgagtg ccacgatcgc
      781 atcggtcgag gcatctggcg ccctggtgaa caggcgcagc agtgcagcac cggtgagcag
      841 gccaatgccg gggatggagg tgatcacgcc gaaggcggta tcgccctggg gcaactcgcg
      901 aaccgcctgg gcaatggcac gctcgaagtc ctgcaaggcg gccttcatct catgcaggag
      961 cttggccagc atggcggctg gcttgggccg cttggccacc gactgcttca gcgcggcata
     1021 tgttttctgc agcgcagccc gctcgcgcag cagatcgtcc agctcttgct gctgctgggt
     1081 cggcggctgc catgcgcgca ggtgctcgtg ctcacgctcg atgaagcgtg cgatcacgcg
     1141 ggcatccagc cgatcggtct tgcctcgctg gcccagccct tgggcataac gcttgatgtc
     1201 gcgcggattg agcacgtaca ccgtgatgcc gcgttcatgg gccagtcggg ccagctgcag
     1261 gtgatagctg cccgtggatt ccaagccgag gcggctgttc gccggagccg atcgaagcca
     1321 cttggcaatg gccttgggat cgttgccaat gcgctgcacc tgctggctgc cgtgccgggc
     1381 gatgtccagg tgctcgctgg ccacgtccac tccccagcag gtttcgttcg gaatctgttc
     1441 cttcatttgt caaaatgccg gggccggagt ggggctcttg cagcggcgtc agcttgtata
     1501 aatcggcgct cgcctgaagg cggcgctagg ttcctaatcg acgctcggtg ccagagtggg
     1561 gctttaactc agtcggtcgg tcgaacgtgc ctcgttcgcc gatgcctgtc gatgtccctc
     1621 cactccggca ccttgccctt tcttgatggt tgctgttgag gttgcaaacc atacaagcct
     1681 gtcgaagggc gcgagtgctt ccgttcgccc tgagcctatc gaagggcttt tcccctcaga
     1741 actcctccgt atccacctcg ctgcgcgatt gctggttgta gcggttgccg ctcaccgtgc
     1801 gctcgttgat gtgcgcctcc agccgcgcca tcagatccgg cgacaggcgc acgtccaccg
     1861 cggccaggtc ttcgcgcaaa tgctccacgc tcgtcgttcc gggaatcgga atgatgtcct
     1921 cgcccttgtg cagcagccag gccagggcca gctgcgccgg ggtgcagccg gcctcctgcg
     1981 ccaaagcctt gtaccccggc agcagcttca ggttggcggc gtagttcgcc ggctcgaagc
     2041 gcggcatgcc gcggcggatg tccttctcgt gcaggctgcc gacgtcgtgc agcgaatcgc
     2101 acaggaagcc gcgcgccacc gggctgaagg ccacgaaggt cgcgcccagt tcgcggcagg
     2161 cggccagcac cgcgatctcg gggttgcggg tccacagcga atactcggtc tgcaccgccg
     2221 cgatggggtg caccgcgtgc gccttgcgca gcgtcgccgc agatacttcc gacaggccga
     2281 tggcgcgcac cttgccctgc tccaccaggc ggctcatcgc gcccacgctc tcctcgatgg
     2341 gcaccttctt gtcccagcgg tgcaggtagt acaggtcgat cacgtcggtc tgcaggcgcc
     2401 gcaggctgtc ctcgcagtgc cgcatgatgc tctcgggccg gccgtcgatc acgcgccgca
     2461 tcgcgccgtc gtcgccggcg atcccggcga gcacgccctt
//
LOCUS       DATLGF010000162         2570 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_147053, whole genome shotgun sequence.
ACCESSION   DATLGF010000162 DATLGF010000000
VERSION     DATLGF010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147053"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VM406_04900"
     CDS             <1..645
                     /locus_tag="VM406_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVL75333.1"
                     /translation="TDDKGAAQKVVFDKLIVSIGRVPNTIGLNAEAVGLQLDERGFIA
                     VDGDCRTNLPNVWAVGDVVRGPMLAHKAEEEGVAVAERIAGQHGHVNFNTIPWVIYTS
                     PEIAWVGRTEQQLKAEGVAYKAGTFPFMANGRARALGDTSGMVKFLADAKSDEILGVH
                     IVGPMASELIAEAVVAMEFRASSEDIARICHAHPSLSESTKEAALAVDKRSLNF"
     gene            655..1062
                     /locus_tag="VM406_04905"
     CDS             655..1062
                     /locus_tag="VM406_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75334.1"
                     /translation="MRYTVLLLSLALAACATTGPQERGIAIETATGGQPLAGTQCTVS
                     TAAGSWEVVTPAVIDVGAPSGDLRVVCNRPGFRTSETIHRAYLPAGPGPTVGVGIGGG
                     SRHTGASIGMSVPFGVGGQASYPRHIRVDMNPL"
     gene            1187..2284
                     /gene="zapE"
                     /locus_tag="VM406_04910"
     CDS             1187..2284
                     /gene="zapE"
                     /locus_tag="VM406_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HVL75335.1"
                     /translation="MNVREYYEHALAERGYQSDPAQVRAVERLQQAYDDWVAYKSQRS
                     NRLMRLISRPQVPRGLYMWGGVGRGKSFLMDSFYSVVPVKRKTRLHFHEFMRDVHRQL
                     DELKGVADPLHEVARRIARKHRLICFDEFHVSDIADAMILYNLLKALFDNGVSFVMTS
                     NYVPDALYPDGLHRDRILPAIALLKARMDVLNVDAGNDYRKRALEQVEAYHTPLGAQA
                     DRALRKAFAKLADTADEDPRLHIEAREIRALRRAGGAVWFDFATLCGGPRSQNDYLEI
                     ASRFHTVILSDVPRMSAAMSSEARRFTWLIDVFYDHKVKLLISAEVAPEELYTEGTLA
                     HEFHRTVSRIIEMQSAEYMQSERRSSAATLS"
     gene            2295..>2570
                     /locus_tag="VM406_04915"
     CDS             2295..>2570
                     /locus_tag="VM406_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75336.1"
                     /translation="MDALPVFATACRALALYLALSLTASAQEQQPTPGAIASLEAAEQ
                     ALEMVGAERGRIEAQFAEEEYACHARFFVSRCLDEAHERRRQALAPLN"
BASE COUNT          458 a          820 c          844 g          448 t
ORIGIN      
        1 accgacgaca agggcgcggc gcagaaggtc gtcttcgaca agctgatcgt ctcgatcggc
       61 cgcgtgccga atacaatcgg cctgaatgcc gaagcggtcg gtttgcaact ggatgagcgc
      121 ggcttcattg ccgtcgatgg cgattgcagg accaacctgc cgaatgtctg ggcagtcggt
      181 gacgttgtgc gcggcccgat gctcgcgcac aaggccgagg aagagggtgt cgccgtggcc
      241 gagcgcattg ccggccagca cgggcacgtg aacttcaata cgatcccctg ggtcatatat
      301 acgtctccgg aaatcgcctg ggtgggccgc accgagcagc agctgaaggc cgaaggcgtc
      361 gcctacaagg ccggcacctt cccgttcatg gccaacgggc gtgcccgcgc gctgggcgat
      421 acctccggca tggtgaaatt cctggctgac gccaagagcg acgaaatcct cggcgtacac
      481 atcgtcggtc cgatggcgtc ggaactgatc gccgaggcgg tcgtcgcgat ggagttccgt
      541 gcctcgtcgg aagacattgc ccgtatctgc cacgcgcatc cttcgctgtc ggaatcgacc
      601 aaggaggcgg cgctggcggt ggacaaacgt tcgctcaact tctaggagtc acgcatgcgc
      661 tacacggtcc tgttgttgtc cctggccctg gctgcctgcg cgacgacggg gccgcaggag
      721 cgcggcatcg caatcgagac cgcgaccggc gggcagccgc tcgccggcac gcagtgcacg
      781 gtcagcacgg ccgctggaag ctgggaggtc gtgacgccgg ccgtaatcga tgtcggcgcg
      841 ccgagcggag acttgcgggt cgtctgcaat cgcccgggct ttcgcacctc cgagaccatc
      901 catcgcgcct atcttccggc cggaccgggg ccgaccgtgg gggtgggcat cggcggcggt
      961 tcgcgccata ccggcgccag catcgggatg agcgtgccgt ttggcgtggg cggacaggcg
     1021 agctatccgc gccacatccg cgtcgacatg aatcccctgt aaagcggatg cagggctgat
     1081 cgtattcaaa tgcaagggcg gactggttcc gcccgtttta ttgacggtgc ctgcgcggca
     1141 gccgaaatgc cgggaggaag ccggcgagcg ggttgaacat aacgatatga acgtcaggga
     1201 gtattacgag catgcgctcg ccgagcgcgg ctaccagtcg gatccggcgc aggtgcgcgc
     1261 cgtcgagcgc ctgcagcagg cctatgacga ctgggtagcc tacaaatcgc agcgctcgaa
     1321 tcggctgatg cgcctgatca gccggcccca ggtgccgcgc ggcctttaca tgtggggcgg
     1381 tgtcgggcgt ggcaagtcct tcctgatgga cagcttttac tcggtcgttc cggtcaagcg
     1441 caagacgcga ctccacttcc acgagttcat gcgcgacgtg catcggcagc tcgacgaact
     1501 caagggcgtg gccgatccgc ttcatgaggt ggcgcgccgc atcgcccgca agcaccgcct
     1561 gatctgcttc gacgagttcc acgtgtccga catcgcggac gcgatgatcc tgtacaacct
     1621 gctcaaggcc ttgttcgaca acggcgtgtc cttcgtcatg acctcgaact atgtgccgga
     1681 tgcgctgtat cccgatggcc tgcatcgtga tcggatcctg ccggcgatcg cgctgctgaa
     1741 ggcacgcatg gacgttctca acgtcgatgc cggcaacgac taccgcaagc gcgcgctcga
     1801 acaggtcgag gcgtaccaca cgccgctcgg tgcgcaggcc gaccgcgcat tgcgcaaggc
     1861 gttcgcgaag ctggccgaca cggccgacga ggatccgcgc ctgcacatcg aagcccgcga
     1921 gattcgcgcg ctgcgccgcg ccggcggcgc ggtatggttc gacttcgcga ccctgtgcgg
     1981 cgggccgcgc tcacagaacg attacctcga gattgccagc cgcttccaca ccgtgatcct
     2041 ctcggacgtg ccccggatgt cggcggcgat gtcgtcagaa gcgcgccgct tcacctggct
     2101 gatcgacgtc ttctatgatc acaaggtcaa gctcctgata tcggccgagg ttgcgcctga
     2161 agagctctac accgaaggta cgctggccca cgagtttcat cgcactgtct cgcgcatcat
     2221 cgaaatgcag tcggccgagt acatgcaaag cgaacgccgc agcagcgcgg ccacactctc
     2281 ttgaggaccc gatcatggat gcgcttccag ttttcgcaac agcctgccgc gcgctcgcac
     2341 tgtacctggc attgtcattg acggcgtctg cccaggaaca gcagccgacc cctggggcga
     2401 tcgcttcgct ggaggcggcc gaacaggcgc tggagatggt gggcgcggag cgcggacgga
     2461 tagaggcgca gtttgccgaa gaagagtatg catgccatgc gcgctttttc gttagccgct
     2521 gcctcgacga agcgcacgaa cggcgccgcc aggcgctcgc gccattgaac
//
LOCUS       DATLGF010000163        20266 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_114712, whole genome shotgun sequence.
ACCESSION   DATLGF010000163 DATLGF010000000
VERSION     DATLGF010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 20266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20266
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114712"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /gene="nadB"
                     /locus_tag="VM406_04920"
     CDS             complement(<1..1175)
                     /gene="nadB"
                     /locus_tag="VM406_04920"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HVL75337.1"
                     /translation="MNFDVAIIGSGLAGLSVALHLAQTRKVVVVSKRSLLDGASNWAQ
                     GGIAAVLDSKDSHEEHIADTLVAGAGLCDEGATRFIVEHSREAIEWLIAQGVPFTKDA
                     TAELGFHLTREGGHSQRRIIHAADATGHAVQLTLVEKVRNHPNITILERHCAIDVIKS
                     DRLDGHEGPPRCVGVYVQDVDNGEVMTLAADHTVLATGGAGKVYLYTTNPDTATGDGI
                     AMAWRAGCRVANMEFIQFHPTCLYHPYAKEFLISEAVRGEGGLLKLPAAAGAAAGHRF
                     MPDHDARAELAPRDVVARAIDFEMKKRGLDYVDLDISHQSPEFLKEHFPTIYARCLEL
                     GIDITKQPIPVVPAAHYTCGGIITDLAGRTDLPGLYAVGEVACTGLHGANRLASNSLL
                     "
     gene            1253..3682
                     /locus_tag="VM406_04925"
     CDS             1253..3682
                     /locus_tag="VM406_04925"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HVL75338.1"
                     /translation="MKRAFGILGLVILAALVALAGAALWYRSASQPKINGSSQVQGLQ
                     APIDIVRDAEGIPHIYAQSSEDAYFGLGYVHAQDRLWQLELNRRIPAGRMAEILGPNA
                     VDTDRFLRTLGVRRNAEAILANLAPETRAALDAYARGINAYLADRSGPLPPEFLLTGA
                     PAPEPWQPVDSIGWQTMMAWDLGANWTQEVLRMRLAQRLSLEQINEFLAPYPGESVPQ
                     TMDYTALYRKLAGTSEQLAALIQAAPPSYVDGMGSNNWVVSGALAESGKPLLANDPHL
                     GLSAPALWYFAHMSAPGMNVIGATLPGLPTVVLGRNDRIAWGFTNTGPDVQDLYIERI
                     NPDNPRQYQTPEGWTNFEVRSETIKVKGQPDVTVEVRATRHGPVLTGALPLFAKAGLD
                     ANHVISFAWTALRPDDLTLQAGIKFGRARNWAEFLDGARDFSSPQQSMVYADVDGNIG
                     FIAAGRVPVRKPENDLKGLAPAPGWDARYDWAGFIPFEDLPQAYNPESQRVMTANHKI
                     VADDYPHFLTSEWTLPYRAQRIDQLLDARDRHSLDSFAAIQKDHLSLAAAELLPLVRN
                     TTVRSERGRAALKLLQGWNGSMDAERSEPLIFNAWMRFASFRIFVDELGEPLLRDYWE
                     QRNVHQAMVNVLSDREGQSRWCADVSRRDAVDAQACSAVLADALEDALDDLEQRYGRN
                     MEKWQWGHAHFARSEHRPFARVPALARFFDIRVPTPGDTHTVNVGRHNLRDEAMPYAN
                     RHAASLRALYDLSNLEDSRFIHSTGQSGNVLSPLYRNFTQRWANVEFVRMRTERTAVE
                     QGSIGTLRLVP"
     gene            3993..5171
                     /locus_tag="VM406_04930"
     CDS             3993..5171
                     /locus_tag="VM406_04930"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019718767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HVL75339.1"
                     /translation="MTDAVIVSTARTGLAKSWKGAFNMTHGATMGGHVLKAAIERAGI
                     DAAEVEDVIMGCATPEGATGSNIARQIALRGGCPVTTAGMTINRFCSSGLQTIATAAQ
                     RVIAGEGDIYAAGGVESISCVQNEANRHMIVEAWLKQNKPEVYWPMLQTAEMVAKRYN
                     IPKERQDEYGVQSQQRAAAAQAAGKFNDEIVPMTTVMGVADKETGMLVTREVTISADE
                     GIRADTTLEGVSKIRTALPGGVITAGNASQFSDGAACTIVMNSKLAEQKGLQPLGIFR
                     GFAVAGCEPDEMGIGPVFAVPKLLKKAGLKVEDIGLWELNEAFAVQVLYCADKLGIPM
                     DRLNVNGGAIAVGHPYGVSGARLTGHALIEGKRRGAKYVVVTMCIGGGQGAAGLFEVV
                     "
     gene            5373..7184
                     /locus_tag="VM406_04935"
     CDS             5373..7184
                     /locus_tag="VM406_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HVL75340.1"
                     /translation="MQSKIIPRRDLDFLLYEWLDAEALTQRARFADHSRETFNAALDT
                     CERIATELFAPHNKKADQNEPHFDGETVHIIPEVKTALKAFSEAGLMAAGQDYEYGGM
                     QLPCLIEKAGFAYFKGANVGTSSYPFLTIGNANTLLKCGTPEQIETFVKPMLEGRFFG
                     TMCLSEPQAGSSLSDIVTRAEPQPDGTYRIKGNKMWISAGEHELSENIVHLVLAKVPD
                     ENGKLIPGVKGISLFIVPKKLVNSDGSLGERNDVVLAGLNHKMGYRGTTNCLLNFGEG
                     KFKPGGQAGAVGYLVGEVHKGLANMFHMMNEARIGVGTGAVMLGYTGYLHALEYARER
                     PQGRHPMNKDPAQPQIPIIQHTDVKRMLLAQKAYVEGGMALNLYCARLVDDERTAPDA
                     DERRRAALLLEILTPIAKSWPSQWCLEANNLAIQVHGGYGYTREYNVEQFYRDNRLNP
                     IHEGTHGIQALDLLGRKVVMKDGAAFALLAERMRQTIAQARDTELAGYAQALEATLAR
                     MASVTRTLYAAGDMNKTLANASAYLEAFGHMVVAWIWLEQGLRALGKDGHDADFYRGK
                     LQACAYFFRWELPKTAPQLDLLESLDTTTLDMQDAWF"
     gene            7351..7653
                     /locus_tag="VM406_04940"
     CDS             7351..7653
                     /locus_tag="VM406_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HVL75341.1"
                     /translation="MLKHIVMWKLKDQAEGADRATNARKMKEMLEACRHIVPGILKLE
                     VGLAQPGLEATYDVVLYSEFESKAALDAYQEHPDHVALKPFFGAVREARQCMDYEV"
     gene            7676..8461
                     /locus_tag="VM406_04945"
     CDS             7676..8461
                     /locus_tag="VM406_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL75342.1"
                     /translation="MRTTQQLFDLAGRTALVTGGSRGLGLQIAEALGEQGAKLVLTSR
                     KQADLDEAVAHLKERGIEASAIAADVSRDENIAPLVEEAIKRLGHIDILINNAGATWG
                     APAEDHPVEAWDKVMNLNVRSIFLLSQAVAKASMIPRKYGRIVNVASIAGLAGNAPGS
                     MQTIAYNTSKGAVVNFTRALAGEWGPHGITVNALAPGFFPSKMTKGVLENLGVENLKR
                     RAPLQRIGDDEDLKGAALLFASDAGKHITGQILAVDGGVSAVH"
     gene            8461..8892
                     /locus_tag="VM406_04950"
     CDS             8461..8892
                     /locus_tag="VM406_04950"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HVL75343.1"
                     /translation="MTRANPFPVEIPFLDDLGVEFLGMAAGEAQVALNLDERHMNSWM
                     VTHGGVTMTLLDVVMSMAGRSLDPEARGGVTVEMKTSFLQPGGKPGSRIIAKGRAFHR
                     STTMCFCEGEVWNGDKLVAKGMGTFKYLKRLDVAKKMEHGT"
     gene            8915..9910
                     /locus_tag="VM406_04955"
     CDS             8915..9910
                     /locus_tag="VM406_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HVL75344.1"
                     /translation="MSTYKRIVLASRPEAEVTPSNFRLEEVPVPEIGDGEILIRNHYL
                     SLDPYMRMRMTTAKSYAEPQPLDQTMIGGTVGEVVASKHPKFKVGDKVQGMLGWAEMG
                     VSDGTMLRTLDTTHVPLSAYLGAVGMPGMTAWYGLTQIMQPKEGETIVVSAASGAVGS
                     VVGQLAKLRGCRAVGIAGGKEKCAYVTDELGFDACIDYKAGQLEADLAKATPDGIDAL
                     FENVGGAGMDACLARMNPFGRIALCGLIAGYDGQPMPIYNIGKVLTMRLTMRGFIISE
                     HMDLWPQGLRELGTLVATGKLKFRESLAQGLEAAPEAFIGMLKGRNFGKQLVRLV"
     gene            9937..10845
                     /locus_tag="VM406_04960"
     CDS             9937..10845
                     /locus_tag="VM406_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL75345.1"
                     /translation="MKNFEGRVAVITGAGSGFGREFARIGARLKMKLALADVQQDALD
                     ATRAELEQQGAEVLALRCDVRKADEVQALADATMQRFGAVHLVFNNAGVGHGGLVWET
                     SVADWEWVLGVNLWGVIHGVRIFTPLMLETAKKEPGYEGHIVNTASMAGLLNAPNMGV
                     YNVSKHAVVSLSETLYHDLQLIDAPIGASVLCPYFVPTGIHQSMRNRPDELKDDAPMT
                     ASQRASRAVSEKAVTSGKVSAAEVAEKTFDAIRSGGFYIYSHPQALGNVQTRMEDIIT
                     QRNPTDPFAAAPQVRDMLKAQLQAGG"
     gene            10848..11747
                     /locus_tag="VM406_04965"
     CDS             10848..11747
                     /locus_tag="VM406_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL75346.1"
                     /translation="MGAPITEGQYASLPNSIRLHYASAGEKGRPLLLFVHGFPEFWFE
                     WEAQLSEFGNDWYAVAPDLRGYNLSDIPREVSAYKAKHLIEDLQQLIAHLGYERCVMV
                     AHDWGGAVAWSLAIAAPARLHKLVIINSPHPYLFARDLSNDPEQQAASQYMNWLRKEG
                     SEDALAKDDFARIEALLTNMGVSATPWFTPEVRAKYRSCWARGLAGGVNYYRASPLYP
                     PLHDDPGARRLRLDPKDFRVHVPTLVIWGERDLALRTSLLDGLSDFVHDLTVERIADG
                     THWVIHEQPERVNRLLRAFLSSA"
     gene            11870..12307
                     /locus_tag="VM406_04970"
     CDS             11870..12307
                     /locus_tag="VM406_04970"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HVL75347.1"
                     /translation="MAREHFSFFHTLRVRWAEVDMQAIVFNGHYLTYFDVAFTEYWRE
                     TGLPNVLDQAAEGRELFARKATVEYHAPARFDDVLDIGVRCAAFGRTSVRFMLEIHRG
                     DEHLISGELVYVYADTAIRKGVALPQDWRETLVRFEKIAPEVA"
     gene            12304..12726
                     /locus_tag="VM406_04975"
     CDS             12304..12726
                     /locus_tag="VM406_04975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL75348.1"
                     /translation="MSIKVTLGDWETQREGAQAVRYQVFVVEQKVPLEMEWDEMDARC
                     VHALVLDDEARPVATGRLLPDGHIGRMAVLPRARGNGVGAAVLQALMKAAKARGDTRV
                     MLNAQTHAEPFYARFGFVRDGEEFEEAGIPHIAMQCRI"
     gene            complement(12768..13493)
                     /locus_tag="VM406_04980"
     CDS             complement(12768..13493)
                     /locus_tag="VM406_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HVL75349.1"
                     /translation="MCLIVFAWKLVPGMPLLAAGNRDEFYDRPAATAEWWDDHPQIYA
                     GRDLRGGGTWMGITRGGRFAAVTNIRAPLDKRQEAPSRGGIVTDFLRGDDSPERYVAA
                     LSERAGHYNGFNLLVGDREQLIWYSNSHADDPRNGRPLEPGIYGLSNGTLDAPWPKVV
                     RSKAQFASLLCQGAPEDAYFEMLTDTTRARDCRLPKTGVDIEWERILSAVCIESPVYG
                     TRASTVVQLFDQGAPVLRERAIR"
     gene            complement(13493..13777)
                     /locus_tag="VM406_04985"
     CDS             complement(13493..13777)
                     /locus_tag="VM406_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4936 family protein"
                     /protein_id="HVL75350.1"
                     /translation="MARHLYVYYRVPVEHASQMHQRAQALLKSISRECGIVARLQRRP
                     EEKDGRQTWMEVYEAVPAGFEETLERAAEREGMTGLIDGQRHTEIFVDVT"
     gene            complement(13786..14844)
                     /locus_tag="VM406_04990"
     CDS             complement(13786..14844)
                     /locus_tag="VM406_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HVL75351.1"
                     /translation="MTDLNHPWLQFLAANGARLDDGQTGEVLGFDSINTDATDPAQGY
                     AVPLTELGLIEATGADAAGFLHNQLTNDIEHLDAAHARRAGYCTPKGRLLATFLVWRT
                     ADAIMLQLPRALQPAVQKRLQIFVLRAKARLADAGADQVAIGLGGKRAAEALAPWFAT
                     PPDAPLAKIDNEAGTLIRQPDAFGQPRWMWIAPLALAQQAWPALSASLAPSPAHVWRR
                     ADIEAGVPQVVAQTQELFVPQMINFELVDGVSFKKGCYPGQEIVARSQYLGKQKRRML
                     PARVASDSVAPGMELFASADPGQPCGMVVNAAPAPSGGAELLVEIKLAAREEGSVHLG
                     AADGPALEFLPLPYPLPQ"
     gene            15024..16013
                     /gene="mltG"
                     /locus_tag="VM406_04995"
     CDS             15024..16013
                     /gene="mltG"
                     /locus_tag="VM406_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HVL75352.1"
                     /translation="MRKLFVYLFLLGLVAAVIFAVWAYRPLVPSNGTPIEFEVRAGSS
                     VRSTMRQMVAGGVPVNPWMMEALARLSGKSSRLKAGFYEVTPGTSPLALIDKLARGDY
                     VTATVTIIEGWTFRQMRAAIAAQPALRHHTSKLSDSELLAKIGSEFKHPEGLFFPDTY
                     RFAKGTSDLQVFRQAHALMQRRLNEVWERRDPSLPYKSPYDVLIMASIIEKETGQSDE
                     RNLVAAVFVNRLRLGMLLQTDPTVIYGMGDRFDGNIRKRDLTTDTPYNTYTRAGLPPT
                     PIALPGAASLLAAVTPAESNALYFVSRGDGTSQFSTNLADHNRAVNKYQRSRR"
     gene            16039..16653
                     /gene="tmk"
                     /locus_tag="VM406_05000"
     CDS             16039..16653
                     /gene="tmk"
                     /locus_tag="VM406_05000"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HVL75353.1"
                     /translation="MKSGKFITFEGIDGAGKSTHVAAVSEQLRALGKTVVLTREPGGT
                     PLGERLRELLLHESMHLETEALLMFASRREHLAQVIEPALARGDWVISDRFTDASFAY
                     QGGGRKLPLDKLRVLEQWVHPHLQPDLTLLFDVPIDVARARLDGSRTLDKFEQEKADF
                     FGAVRAGYLQRAADFPERFRVIDSTRPLETVREEVRGVIAALAT"
     gene            16650..17690
                     /locus_tag="VM406_05005"
     CDS             16650..17690
                     /locus_tag="VM406_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HVL75354.1"
                     /translation="MNDFFPWHEAAWRQLQQMLGRLPHAILLHGPQGTGKTAFAEAFA
                     QSLLCTNRHQDGRACGACEACGWFAQYAHPDYRRVRPEALDDEPGEGGEGEDGGDGDT
                     KKSAKSKTPSKEIKIDQIRALGDFMNVSTHRQGLRVVLLYPAEALNTMAANALLKTLE
                     EPPPATVFILVSHAIERLLPTILSRCRKFALPMPPRAQALAWLKEQGVADADDWLAEQ
                     GGAPLSAIAASASAVRELQDEFLRALARPDTELALKTAERLQKAPVSDLVLWQQRWLY
                     DVLAHKLSGTIRYYPRYRRELESLSRRAPAAALLAAVKRANERRAVAEHPLAARLFIE
                     DLLLDYTAVFAS"
     gene            17709..18098
                     /locus_tag="VM406_05010"
     CDS             17709..18098
                     /locus_tag="VM406_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HVL75355.1"
                     /translation="MPDPNTISPSASGAAGAVRPSVLSLPIKEKAALYAAYMPYLANG
                     GIFVPTNKPYKIGDEIYLILTLMDDPTKYPIAGKIAWITPAGANNNKAQGIGVHFSSD
                     ESGQRVKLRIEELLGAALRSTRATHTL"
     gene            18133..18630
                     /locus_tag="VM406_05015"
     CDS             18133..18630
                     /locus_tag="VM406_05015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL75356.1"
                     /translation="MAFSHRLARFEDLPAIVAIYNSTVASREVTADTEPITVESRHAW
                     FAEHTPDRRPLWVAEQDGEIIGWLSYSNFYGRPAYAGTSELSIYIAENARGKGLGRYF
                     LEQAIAHAPKIQVHTLLGFIFGHNMPSLALFERFGFERWAHLPRVATLDGIERDLVIL
                     GKRVA"
     gene            18666..19451
                     /locus_tag="VM406_05020"
     CDS             18666..19451
                     /locus_tag="VM406_05020"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HVL75357.1"
                     /translation="MYIDSHCHINFPELAGRLPEIFARMADKQVTHALCVSVNLPEFP
                     QVLALAEQHPNIFASVGVHPDYEDSPEPTVEELVRLSGHPKIVAIGETGLDYYRLEGD
                     LEWQRERFRTHIRASRATGKPLIIHTRAASEDTIRIMREEGAGTDAGGAGGVMHCFTE
                     SLEVAQAAMEMGFYISFSGIVTFKSARELQAVAQAVPLERMLIETDSPYLAPVPYRGK
                     TNEPGFVAHVGEFLATLKDVPVEQLARQTSANFFNLFKTAKSL"
     gene            19448..20143
                     /locus_tag="VM406_05025"
     CDS             19448..20143
                     /locus_tag="VM406_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HVL75358.1"
                     /translation="MSATAADSSAAMRRRMLVAGLACMLGVVAPPAAFAQSSYVAYFR
                     AVKIDNTRTVGSLLARGFDPNTIEPDRGETGMMLALREGAPGVFRLLLEHPETNVNVR
                     AFNGDSALMIACYTGNGEAARALIERGAAVHHPGWTPLHYAAAGGHNDIAQMLMALGA
                     PPDARSPNNTTPMMMAALGGHIMTVKVLLDAGADATLKNDHGMTAIDFARRNDHQDIV
                     DGLIWRLRRAGKL"
     gene            complement(20121..>20266)
                     /locus_tag="VM406_05030"
     CDS             complement(20121..>20266)
                     /locus_tag="VM406_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL75359.1"
                     /translation="RDTGGTGLGLTIARNIAEQHGGSVALSNCEGGGLEARLELPTVS
                     RPV"
BASE COUNT         3636 a         6508 c         6680 g         3442 t
ORIGIN      
        1 agcagcgagt tgctggccag gcggttggcg ccgtgcaggc cggtgcaggc gacctcgccc
       61 acggcgtaca ggccgggcag gtcggtgcgg cccgccaggt cggtgatgat gccgccgcag
      121 gtgtaatggg cggcgggcac gaccggaatc ggctgcttcg tgatgtcgat gcccagttcc
      181 aggcagcgcg cgtaaatggt agggaaatgt tccttcagga attccgggct ctggtggctg
      241 atgtcgagat cgacgtagtc gagcccgcgc ttcttcattt cgaagtcgat cgcgcgcgcc
      301 acgacatcgc gcggcgccag ttccgcacgc gcgtcgtggt cgggcatgaa gcgatgcccg
      361 gccgccgcgc cggctgccgc aggcagcttc agcagcccgc cttcgccgcg cacggcctcg
      421 gaaatcagga attccttcgc ataggggtgg tacaggcagg tcggatggaa ctggatgaac
      481 tccatgttcg cgacgcggca gccggcgcgc caggccatcg cgatgccgtc gccggtcgcg
      541 gtgtccggat tggtcgtgta cagatagacc ttgccggccc cgccggtggc cagcaccgta
      601 tggtcggcgg ccagcgtcat cacctcgccg ttgtcgacgt cctgcacata gacgccgacg
      661 cagcgcggcg gaccttcgtg gccgtcgagc cggtccgact tgatcacgtc gatcgcgcaa
      721 tggcgttcga ggatggtgat gttcggatgg ttgcgcacct tctccaccag cgtaagctgc
      781 accgcatggc cggtggcgtc ggcggcgtgg atgatgcgcc gctggctgtg cccgccttcg
      841 cgcgtcaggt gaaatccgag ttcggcggtc gcatccttgg tgaacggcac gccctgtgcg
      901 atcagccact cgatcgcctc gcggctgtgc tcgacgatga agcgggtcgc cccttcgtcg
      961 cacagcccgg cgccggccac cagcgtgtcg gcgatgtgtt cctcgtggct gtccttggaa
     1021 tcgagaaccg cggcgatgcc gccctgcgcc cagttgctgg cgccgtcgag caggctgcgc
     1081 ttggagacaa cgacaacttt gcgggtttgc gcgagatgca gcgcgacgga caggccggcc
     1141 aggccgctgc cgatgatggc gacgtcgaaa ttcatggtgt cgatagtatg cggatcggcc
     1201 acatcttatc attcgtcact gtgatagtct gtgcgcccat ccaggaggaa gcatgaagcg
     1261 tgcgtttgga attctcggtc tggtcatcct tgcggcgttg gtggcgcttg ccggcgccgc
     1321 gctctggtat cgcagcgctt cgcagcccaa gatcaacggc agctcgcagg tgcagggcct
     1381 gcaggcgccg atcgacatcg tgcgcgatgc ggaaggcatt ccgcacatct acgcgcaatc
     1441 gtcggaagac gcgtatttcg gcctcggcta cgtgcatgcc caggaccgcc tctggcagct
     1501 cgaactgaac cgccgcattc cggccggccg catggccgag atcctcgggc cgaacgctgt
     1561 cgacaccgac cgcttcctgc gcacgctcgg cgtgcgccgc aacgccgaag ccatcctcgc
     1621 caacctcgcg ccggaaacgc gcgcggcgct cgacgcctat gcgcgcggca tcaacgccta
     1681 tctcgccgac cgcagcggac cgctgccgcc ggaattcctg ctgaccggcg cgccggcacc
     1741 tgaaccctgg caaccggtcg attcgatcgg ctggcagacc atgatggcct gggacctcgg
     1801 cgcaaactgg acgcaggagg tgttgcgcat gcggctcgcg caacgcctgt cgttggagca
     1861 gatcaacgag tttctcgcac cgtatccggg cgagtcggtg ccgcagacga tggactacac
     1921 ggcgctgtac cgcaagctcg ccggcaccag cgagcaactg gccgcgctga tacaggccgc
     1981 gccgccttct tacgtggatg gcatggggtc gaacaactgg gtggtgagcg gcgcgctcgc
     2041 cgagagcggc aagccgctgt tggcaaacga tccccacctg ggcctgtccg cgccggcgct
     2101 gtggtatttc gcgcacatgt cggcgcccgg catgaacgtg atcggcgcca cgctgccggg
     2161 cttgcccacc gtggtgctgg gccgcaatga ccgcatcgcc tggggattca ccaataccgg
     2221 tcccgatgtg caggatctgt acatcgaacg catcaatccg gataacccgc gtcaatacca
     2281 gacgccggag gggtggacca acttcgaggt gcgcagcgag acgatcaagg tcaagggaca
     2341 gccggacgtc accgttgagg tgcgcgcgac ccggcacggg ccggtcctca ccggcgcgct
     2401 gccgctgttc gccaaggccg gtctcgacgc aaatcacgta atctcgttcg cctggaccgc
     2461 gctacgcccg gacgacctga cgctgcaggc cggcatcaag ttcgggcgcg cacgcaactg
     2521 ggcggaattc ctcgacggcg cgcgagattt ttcgtcgccg cagcagagca tggtgtacgc
     2581 ggacgtcgat ggaaacatcg gcttcatcgc cgccggccgg gtgccggtgc gcaagccgga
     2641 aaacgatctg aagggtctcg cgcctgcacc gggctgggac gcgcgctatg actgggccgg
     2701 cttcattccg ttcgaggacc tgccgcaggc gtataacccg gaatctcagc gcgtgatgac
     2761 ggctaaccac aagatcgttg ctgacgatta cccgcacttc ctgaccagcg aatggacgct
     2821 gccataccgt gcgcagcgta tcgatcagtt gctggacgcg cgcgaccggc acagcctcga
     2881 cagcttcgcc gcaatccaga aggaccacct gtcgctggcc gcggccgaac tgctgccgct
     2941 ggtgcgcaac accactgtcc gatcggaacg cggccgcgcg gcgctcaagc tgctgcaagg
     3001 atggaacggc tcgatggacg cagagcgcag cgagccgctg atcttcaatg catggatgcg
     3061 tttcgcatcg ttccgcatct tcgtcgatga acttggggag ccgttgctgc gcgactactg
     3121 ggagcagcgc aacgtgcatc aggccatggt gaatgtgctc agcgaccgcg aaggacagag
     3181 ccgctggtgt gcggatgtca gccgccggga cgcggtcgat gcgcaggcat gctcggcggt
     3241 gctggccgat gcgctcgagg acgcgctcga tgatctggag cagcgctatg gccgcaacat
     3301 ggagaagtgg cagtgggggc acgcgcactt cgcacgctcc gaacatcgtc catttgcccg
     3361 cgtgccggcg ctggcgcgct tcttcgacat ccgcgtgcct accccgggcg acactcacac
     3421 ggtaaacgtc ggtcgccaca acctgcgcga cgaggcgatg ccgtacgcga accggcatgc
     3481 ggcgagcctg cgcgcgctgt atgacctctc caacctggaa gactcgcgct tcatccattc
     3541 gaccggccag tccggcaatg tgctgtcgcc gctgtaccgc aacttcacgc agcgctgggc
     3601 gaatgtggag ttcgtgcgga tgcgcacgga acgcaccgcg gtggagcagg gcagcatcgg
     3661 cacgctgcgt ctggtgccct gataaaaaaa cggccaccgg aatgaccggt ggccgaaagc
     3721 caggcatgcc acgggagggt gcgcctggga ctccatcgtc agtaaaccgc ctgacggcaa
     3781 tgcgtcaatc ctgcctcctt ttgcttacgt gaaccttgcg tacagtgcct tttcaggcga
     3841 ctttgcgtac gtacccggtc ttccataatt ttgcgaacga ccgtgctaaa actgtgctat
     3901 tctgcattcg tcatcggccc gccgagacgg tgccggcaaa gtttttccca cccacgaatc
     3961 acacatacag cacactgttc aaggagactg aaatgactga cgccgtcatc gtatcgaccg
     4021 cccgtaccgg ccttgccaaa tcctggaagg gcgcattcaa catgacccat ggcgccacca
     4081 tgggcgggca tgtgctgaag gccgcgatcg agcgggccgg catcgacgcg gccgaggtcg
     4141 aggacgtgat catgggttgc gcgacaccgg aaggcgcgac cggcagcaac atcgcgcgcc
     4201 agatcgcgtt gcgtggcggc tgtccggtca ccaccgcggg catgacgatc aaccgtttct
     4261 gctcgtccgg cctgcagacc atcgcaaccg ccgcccagcg cgttatcgca ggcgaaggcg
     4321 acatctatgc ggccggcggc gtcgaatcga tctcctgcgt gcagaacgaa gcgaaccgcc
     4381 acatgatcgt cgaagcgtgg ctcaagcaga acaagcccga ggtctattgg ccgatgctgc
     4441 agaccgccga gatggtcgcc aagcgctaca acatcccgaa ggagcgccag gacgaatacg
     4501 gcgtgcagag ccagcagcgc gccgccgccg cacaggccgc cggcaagttc aatgatgaaa
     4561 tcgtcccgat gaccaccgtg atgggcgtgg ccgacaagga aaccggcatg ctggtcacgc
     4621 gcgaagtgac gatctccgcc gacgagggca tccgcgctga cactacgctc gaaggtgtct
     4681 cgaagatccg caccgcgctg ccgggcggcg tgatcaccgc cggcaatgcc agccagttct
     4741 ccgacggtgc agcctgcacg atcgtaatga acagcaagct tgcggaacag aagggcctgc
     4801 agcccctggg catcttccgc ggtttcgcgg ttgccggctg cgagccggac gaaatgggca
     4861 tcggtccggt gttcgcggtg cctaagctgt tgaaaaaggc cggtctgaag gtcgaggaca
     4921 tcgggctgtg ggaactgaac gaggcgtttg cggtgcaggt gctgtattgc gcggacaagc
     4981 tcggcattcc gatggatcgt ctgaacgtga acggcggcgc gatcgccgtc ggccatccat
     5041 atggcgtatc cggcgcgcgc ctgaccggcc atgcgctgat cgaaggcaag cgccgcggcg
     5101 cgaagtatgt ggttgtgacg atgtgtatcg gcggcggaca gggggctgcg gggttgtttg
     5161 aggtggtttg ataccgggct gtcgcttcgc ccgcggccgg gctccttccc cctttgaggg
     5221 ggaaggttgg gatggggtgc gaaggtcatg tataggccag cgcttgaaaa cctccgtacc
     5281 ctccctggcc ctccccctgg aagggacgag ggaaccggtc aacggtggcg cgcccaaagc
     5341 acgttctcat tgcattcgcc cctggagccc ccatgcaatc caagatcatc ccccgccgcg
     5401 acctcgactt cctgctctac gaatggctgg acgccgaggc gctcacgcag cgcgcgcgct
     5461 tcgccgatca ctcgcgcgaa accttcaacg ccgcactcga cacttgcgag cgcatcgcca
     5521 ccgaactgtt cgcgccccac aacaaaaaag ccgaccagaa cgagccgcat ttcgacggcg
     5581 agaccgtgca catcatcccc gaggtcaaga ccgcgctgaa ggctttcagc gaagccggcc
     5641 tgatggccgc cgggcaggac tacgaatacg gcggcatgca gctgccctgc ctgatcgaga
     5701 aggctggctt cgcctatttc aagggtgcca atgttggcac ctcgtcctac ccgttcctga
     5761 ccatcggcaa cgccaacacg ctgctcaagt gcggcacgcc cgagcagatc gagaccttcg
     5821 tcaagccgat gctcgaaggc cgcttcttcg gcacgatgtg cctgtccgag ccgcaggcgg
     5881 ggtcctcgct gtcggacatc gtcacgcgcg ccgagccgca gccggacggc acctaccgca
     5941 tcaagggcaa caagatgtgg atctcggccg gtgaacacga gctgtccgag aacatcgtgc
     6001 atctggtgct ggccaaggtg ccggacgaga acggcaagct gatcccgggt gtgaagggca
     6061 tctcgctgtt catcgtgccg aagaaactgg tcaattcgga tggctcgctc ggcgagcgca
     6121 atgacgtcgt gctggcgggc ctgaaccaca agatgggcta tcgcggcacc accaactgcc
     6181 tgctgaactt cggcgagggc aagttcaagc cgggcggcca ggccggtgcg gtcggctacc
     6241 tggtcggcga ggtgcacaag gggctcgcca acatgttcca catgatgaac gaggcccgca
     6301 tcggcgtggg taccggcgcg gtaatgctcg gctataccgg atacctgcac gcgctggagt
     6361 atgcgcgcga gcggccgcag ggccgccatc cgatgaacaa ggatccggcg cagccgcaga
     6421 ttccgatcat ccagcacacc gacgtcaagc gcatgctgct tgcgcagaaa gcgtatgtcg
     6481 aaggcggcat ggcgctgaac ctgtactgcg cgcgcctggt cgatgacgag cgcaccgcgc
     6541 cggatgcgga tgaacgccgc cgcgccgcgc tgctgctcga gatcctgacc ccgatcgcga
     6601 agtcctggcc ttcgcagtgg tgtctggaag caaacaatct cgcaatccag gtgcacggcg
     6661 gctacggcta tacgcgcgag tacaacgtcg agcagttcta ccgcgacaac cgtctgaacc
     6721 cgatccacga aggcacgcac gggatccagg cgctcgacct gctcgggcgc aaggtggtga
     6781 tgaaggatgg cgccgcattt gcgctgctgg ccgagcggat gcgccagacg atcgcgcagg
     6841 cgcgcgatac ggaactcgcc ggatacgcgc aggcgctgga agcgacgctc gcacggatgg
     6901 cgtcggtcac gcgcacgctg tatgcggcgg gagacatgaa caagaccctg gccaatgcca
     6961 gcgcatacct ggaagcgttc gggcacatgg tggtggcctg gatctggctg gagcagggct
     7021 tgcgcgcgct cggcaaggac gggcatgacg ccgatttcta ccgcggcaag ctgcaggcct
     7081 gcgcctactt cttccgttgg gaattgccga agaccgcgcc gcagcttgac ctgctggaaa
     7141 gcctggacac gactacgctg gacatgcagg atgcgtggtt ctaattaaaa cccgcctcgg
     7201 taggattccc tccccctttc agggggaggg ttagggtggg ggtgatgaag tcgagcaagt
     7261 acttgctgaa aatcggcctc cgtaccccct ccctagccct ccccctgaga gggggagggg
     7321 acccttccgg gcgacgaacg gaaattcacc atgcttaaac acatcgtcat gtggaaactg
     7381 aaagaccagg ccgaaggcgc cgaccgcgcg accaatgcgc gcaagatgaa ggagatgctg
     7441 gaggcatgca ggcacatcgt gcccggcatc ctcaagctcg aggtcgggct ggctcagccg
     7501 gggctggaag ccacctacga cgtggtgctg tactccgaat tcgaatcgaa ggcggcgctg
     7561 gatgcctacc aggagcatcc ggaccatgtc gcgctcaagc ccttcttcgg cgcggtgcgc
     7621 gaggcgcgcc agtgcatgga ttacgaagtt tgatcatcta aaaaaaggag acaccatgcg
     7681 taccactcaa caactctttg atctcgccgg caggaccgcg ctcgtcaccg gcggctcgcg
     7741 cggcctcgga ctgcagatcg ccgaagcgct cggcgagcag ggcgcgaaac tcgtactcac
     7801 ctcgcgcaag caggccgatc tcgacgaggc cgtcgcccac ctgaaggaac gcggtatcga
     7861 ggcgagcgcg atcgccgccg acgtctcgcg cgacgaaaac atcgcgccgc tcgtcgagga
     7921 agcgatcaag cgcctcggcc acatcgacat cctgatcaac aatgccggcg cgacctgggg
     7981 tgcgcccgcg gaagatcatc ctgtcgaagc ctgggacaag gtgatgaacc tgaacgtgcg
     8041 cagcatcttc ctgctgtcgc aggcggtggc caaggcatcg atgattccgc gcaagtatgg
     8101 ccgcatcgtc aacgtcgcct ccatcgcggg cctggccggc aacgcgccgg gctcgatgca
     8161 gacgatcgcc tacaacacct cgaagggggc ggtcgtcaat ttcacccgcg cgctggccgg
     8221 cgaatggggc ccgcacggca tcacggtcaa tgcgctggcg cccggattct tcccgtccaa
     8281 gatgaccaag ggcgtgctgg agaaccttgg ggtcgagaac ctcaagcgcc gcgcgccgct
     8341 gcagcgcatc ggcgacgacg aggatctgaa gggcgcagcg ctgctgttcg cgtccgatgc
     8401 cggcaagcac atcaccggac agattctcgc ggttgatggc ggcgtctcgg cggtgcattg
     8461 atgacgagag cgaatccttt cccggtcgag atcccgtttc tggacgatct cggcgtcgag
     8521 tttctcggta tggccgccgg cgaggcgcag gtggcgctga acctcgacga gcgccacatg
     8581 aacagctgga tggtcaccca tggcggcgtg acgatgacct tgctcgatgt ggtgatgtcg
     8641 atggccggcc gctcgctcga tcccgaggcg cgcggcggcg tgaccgtgga aatgaagacc
     8701 agctttctgc agcccggcgg caagccgggt tcgcgcatca tagccaaggg cagggcgttc
     8761 caccgctcta ccacgatgtg cttctgcgag ggtgaagtct ggaacggcga caagctggtc
     8821 gccaagggaa tgggcacgtt caagtacctg aagcggctgg acgtggcaaa gaagatggag
     8881 cacgggacct gatcattttc ctggaggatc gtcaatgagc acctacaagc gcatcgttct
     8941 tgcctcgcgt cccgaggccg aagtcacccc ttccaacttc cggctcgagg aagtgccggt
     9001 gccggaaatc ggggacggcg aaatcctgat ccgcaatcac tacctgtcgc tcgacccgta
     9061 catgcgcatg cgcatgacca cggcgaagag ctatgcggaa ccgcagccgc tggaccagac
     9121 gatgatcggc ggcaccgtcg gcgaagtggt ggcctcgaag cacccgaagt tcaaggtcgg
     9181 cgacaaggtg cagggcatgc tgggctgggc cgagatgggc gtgtcggacg gcacgatgct
     9241 gcgcacgctc gatacgaccc atgtgccgct gtcggcctac ctcggtgcgg tcggcatgcc
     9301 gggcatgacc gcatggtatg ggctcacgca gatcatgcag ccaaaggaag gcgagaccat
     9361 cgtggtgtcg gcagccagcg gcgcggtcgg cagcgtggtc ggccagctgg cgaaactgcg
     9421 cggctgccgc gcggtcggga tcgccggcgg caaggagaag tgcgcgtacg tgaccgatga
     9481 gctcggcttc gatgcctgca tcgactacaa ggcgggccag ctcgaggctg acctcgcgaa
     9541 ggcgacgccc gacggtatcg acgcgctgtt cgaaaacgta ggcggcgccg ggatggatgc
     9601 ctgcctggcg cgcatgaatc ctttcgggcg gatcgcgctg tgcggactga tcgccggata
     9661 cgacggccag ccgatgccga tctacaacat cgggaaggtt ctgacgatgc ggctgaccat
     9721 gcgcggcttc atcatctccg agcacatgga cctgtggccg cagggactga gggaactggg
     9781 cacgctggtc gcgacgggca agctcaagtt ccgggaatcg cttgcgcaag gcctggaagc
     9841 ggcgccggag gcattcatcg gcatgctcaa gggacgcaat ttcggcaagc agctggtcag
     9901 gctggtctga acaattaatc aaagacagga gacgaaatga agaacttcga gggcagggtc
     9961 gccgtgatca ccggcgccgg aagcggcttc ggcagggagt tcgcacgcat cggcgcacgc
    10021 ctgaagatga agctggcgct ggccgacgtg cagcaggacg cgctggacgc cacacgcgcc
    10081 gagctggaac agcagggcgc cgaagtgctg gcgctgcgct gcgatgtacg caaggcagac
    10141 gaggtacagg cgcttgcgga cgcgacgatg cagcgcttcg gcgcggtcca cctcgtgttc
    10201 aacaatgccg gggtcggcca tggcgggctg gtctgggaaa cttcggtcgc cgactgggag
    10261 tgggtgctcg gcgtgaacct gtggggcgtg atccacggcg tgcgcatctt caccccgctg
    10321 atgctggaga ctgcgaagaa agagcccggc tacgaaggac acatcgtcaa caccgcctcg
    10381 atggcgggcc tgctgaatgc gccgaacatg ggcgtctaca acgtttccaa gcacgcggtc
    10441 gtgtcgctgt ccgaaaccct gtatcacgat ctgcagttga tcgacgcacc gatcggcgca
    10501 tcggtgctgt gcccgtattt cgtccccacc ggcatccacc agtctatgcg caaccgtccc
    10561 gacgagctga aggacgacgc accgatgacc gctagccagc gcgcttcccg cgcggtctcc
    10621 gagaaagcgg tcacctcggg caaggtgagt gccgccgagg tcgcggaaaa gaccttcgat
    10681 gcgatccgca gcggcggctt ctacatctat tcgcatccgc aggcgctcgg caatgtgcag
    10741 acgcgcatgg aggacatcat cacccagcgc aacccgaccg atccgtttgc cgccgcaccg
    10801 caggtgcgcg acatgttgaa ggcgcagctg caggcgggag gctgaacatg ggcgcgccga
    10861 tcacggaagg gcagtatgcg agcctgccca acagcatccg cctgcactat gccagtgccg
    10921 gcgagaaggg ccggccgctg ctcctgttcg tgcacggttt tcccgagttc tggttcgagt
    10981 gggaagccca gctgtccgag ttcggcaacg attggtacgc ggtggcgccg gatctgcgcg
    11041 gatacaacct gtcggacata ccgcgggagg tatcggccta caaggcgaag cacctgatcg
    11101 aagacctgca gcagctgatc gcccatcttg gctacgaacg ctgcgtgatg gtcgcgcacg
    11161 actggggcgg agcggttgcc tggagcctcg cgatcgccgc acccgcgcgg ctgcacaagc
    11221 tggtgatcat caactcgccg catccttatc tgttcgcgcg ggatctttcg aacgacccgg
    11281 aacagcaggc ggccagccag tacatgaact ggctgcgcaa ggaaggctcg gaagacgctc
    11341 ttgccaagga tgacttcgcc aggatcgagg ccctgctgac caacatgggc gtctccgcca
    11401 cgccatggtt cacaccggag gtgcgggcga aataccgcag ctgctgggcg cgtggcctgg
    11461 ccgggggcgt caactactat cgcgcgtcgc cgctgtatcc gccgctgcat gatgatcccg
    11521 gcgcgcgccg cctgcggctg gatccgaagg acttccgcgt gcacgtgccg acgctggtga
    11581 tctggggcga acgcgatctg gccttgcgta catccctgct cgacggcctg agcgacttcg
    11641 tccacgatct gacggtcgaa cggattgccg acggcacgca ctgggtgatc catgagcagc
    11701 cggaacgcgt caaccgcctc ctgcgtgcgt tcctgtccag tgcctgaaaa cgccgctcgc
    11761 aatgcgggcg gcgcgatcag gcgctacact tgcaaggtgt gcgcgcggga gtgcgctttg
    11821 cgcacgcggc gctcctgcgg cgatccacgc tgaacctcat tcctcacaca tggcacgcga
    11881 acacttctcc ttcttccaca cgctgcgcgt acgctgggcc gaagtcgaca tgcaggcaat
    11941 cgtcttcaat ggccactacc tgacgtactt tgacgtcgcc ttcaccgagt actggcgcga
    12001 aaccggcctg cccaatgtgc tggaccaggc cgcagaaggg cgcgagctgt ttgcacgcaa
    12061 ggccacggtc gagtaccacg cgccggcgcg cttcgacgac gtgctcgaca tcggcgtgcg
    12121 ctgcgcggcc ttcgggcgca catcggtgcg cttcatgctc gagatccatc gcggcgacga
    12181 gcacctgatt tcgggggagc tggtctatgt gtacgccgac accgccatac gcaagggcgt
    12241 cgcgctgccg caggactggc gggagaccct ggtccggttc gagaagatcg cgccggaggt
    12301 cgcatgagca tcaaggtgac tctcggtgac tgggagacgc agcgggaggg ggcgcaggcg
    12361 gtgcgctacc aagtcttcgt ggtggagcag aaggtcccgc tggaaatgga gtgggatgag
    12421 atggatgcgc gctgcgtgca tgcgctcgtg ctcgacgatg aggcaagacc ggttgcaacc
    12481 ggccgcctgc tgcccgacgg tcacatcggc cgcatggccg tactgccgcg tgcgcgcggc
    12541 aacggcgtcg gcgcggcagt gctgcaggcg ctgatgaagg cggcaaaggc gcgcggcgat
    12601 acgcgcgtga tgctcaatgc gcagacgcat gccgaaccgt tttatgcgcg gtttggtttt
    12661 gtgcgcgatg gcgaggaatt cgaggaggcg gggattccgc acatcgcgat gcaatgccga
    12721 atctgaggcc gcgaaattct gcgcgtggcc cggcgcttca gatcgttcta ccggatcgca
    12781 cgctcgcgca gaacgggtgc tccctggtcg aatagctgga caacggtgga tgcgcgtgtc
    12841 ccgtagacag gggattcgat gcagaccgcc gagagaatgc gctcccattc gatatccacg
    12901 cccgtcttcg gcaagcggca atcgcgcgcg cgcgtggtgt cggtgagcat ctcgaagtac
    12961 gcgtcctcag gcgccccctg gcacagcagg ctcgcgaact gcgccttgct gcgcaccacc
    13021 ttgggccacg gcgcatcgag cgtcccgttg gacaggccat agatgcccgg ttcgagcggc
    13081 ctgccgttgc gcgggtcgtc ggcgtggctg tttgaatacc agatcaattg ctcgcggtcg
    13141 ccgaccagca ggttgaaacc gttgtaatgg ccggcgcgct cggacagcgc ggcgacgtac
    13201 cgctcgggag aatcatcgcc ccgcaggaag tcggtcacga tgccgccgcg ggacggcgct
    13261 tcctggcgct tgtcgagcgg ggcgcggatg ttggtgacgg cggcgaaccg gccgccgcgg
    13321 gtgatgccca tccaggtgcc gccgccacgc agatcgcgtc cagcatagat ctgcgggtgg
    13381 tcatcccacc attcggccgt ggccgcaggg cggtcgtaga actcgtctcg gttaccggct
    13441 gccaggagtg gcatgccggg gaccagcttc caggcgaata cgatcaggca catcaagtca
    13501 catccacgaa gatttccgta tggcgctgac cgtcgatcaa accggtcatt ccttcgcgct
    13561 cggcggcgcg ctccagcgtc tcttcgaatc cggccgggac cgcctcatag acttccatcc
    13621 atgtctggcg gccgtccttc tcctccgggc ggcgctgaag tctggcgacg ataccacatt
    13681 ccctggaaat cgatttcagc agcgcttgcg cacgctggtg catttgcgac gcgtgttcga
    13741 cggggacgcg gtagtagacg tagaggtgac gtgccatggc aatgcctatt gcggaagcgg
    13801 atacggcagg gggaggaatt ccagcgccgg gccgtcggcc gcaccgaggt gcacgctgcc
    13861 ttcttcgcgc gcggcgagct tgatctctac gagcagctct gcgccgcccg aaggcgccgg
    13921 cgcggcgttg accaccatgc cgcagggctg gcccgggtca gcgctggcga acagctccat
    13981 gcccggtgcg accgagtcgc tggccacccg cgccggcagc atgcggcgct tctgcttgcc
    14041 caggtactgg ctgcgcgcga cgatctcctg gcccggatag caacccttct tgaagctgac
    14101 gccatcgacc agctcgaagt tgatcatctg cggcacgaac agctcctggg tctgcgcgac
    14161 gacctggggc acgcccgcct cgatgtcggc gcggcgccac acgtgggcgg gcgagggcgc
    14221 gaggctggcc gatagcgcgg gccacgcctg ctgtgcgagg gcgagcggcg caatccacat
    14281 ccagcgcggc tgcccgaacg cgtccggctg gcggatcagc gtgcccgcct cgttgtcgat
    14341 cttcgcgagc ggggcgtccg gcggcgtcgc aaaccatggc gccaaggcct cggccgcacg
    14401 cttgccgccc agcccgatcg caacctggtc ggcgccggca tcggccagcc gcgccttggc
    14461 gcgcagcacg aagatctgca ggcgcttctg cacggccggt tgcagtgcgc gcggcagttg
    14521 cagcatgatt gcgtcggccg tgcgccagac taggaaggtt gccagcaggc gccccttcgg
    14581 cgtgcagtag ccggcgcggc gcgcgtgcgc ggcgtccaga tgctcgatgt cgttcgtcaa
    14641 ctggttgtgc aggaaaccgg cggcatccgc gccggtcgcc tcgatcaggc cgagctcggt
    14701 cagcgggacg gcgtagcctt gggccgggtc ggtcgcatca gtgttgatgc tgtcgaatcc
    14761 cagcacctcg ccggtctggc cgtcgtcaag ccgggcgccg ttcgcggcca ggaattgcaa
    14821 ccatggatgg ttcagatcag tcatagcttc ggaaaattgt ggtttcgcat cattgctggc
    14881 ccggccagac tccttgatgg cgcggcgcag ccgcctctgc ggatgaagca aatcgtttcg
    14941 gattagccgg tatcattccg gctcgattat agtgagcccg caacaaacgc gtcgccggac
    15001 gaccggcctg aaccatgtcc ttgatccgaa aactgttcgt ttacctgttc ttgctcggcc
    15061 tcgttgccgc tgtaatcttt gccgtatggg cttatcgtcc cctggtcccg tcaaatggca
    15121 cgccgatcga gttcgaggtg cgggccggca gcagcgtgcg cagcacgatg cgccagatgg
    15181 tggcgggcgg ggtgccggtc aacccgtgga tgatggaagc gctcgcgcgc ctgtcgggca
    15241 agagttcgcg cctgaaggcc ggtttctatg aagtgacgcc gggcacctcg ccgctggccc
    15301 tgatcgacaa gctggcccgc ggcgactacg tgacggccac cgtcaccatc atcgaaggct
    15361 ggacgttccg ccagatgcgc gcggcgatcg cggcccagcc ggcactgcgc catcatacga
    15421 gcaagctgtc tgacagcgaa ctgctggcga agatcggttc ggagttcaag catcctgaag
    15481 gcctgttctt cccggatacc taccgcttcg cgaagggaac cagcgatctt caggtgttcc
    15541 gtcaggccca cgcgctgatg cagcgccggc tcaatgaagt gtgggaaagg cgcgacccca
    15601 gcctgccgta caagagtccg tatgacgtgc tgatcatggc ttcgatcatc gagaaggaaa
    15661 ccgggcagag cgacgagcgc aacctggtgg ccgcggtgtt cgtgaaccgg ctgcggcttg
    15721 gcatgctgct gcagaccgac ccgaccgtca tctacggcat gggtgaccgc ttcgacggca
    15781 acatccgcaa gcgcgatctg accaccgaca cgccttacaa cacctacacc cgggccggcc
    15841 tgccgccgac accgatcgcg ttgccgggcg ccgcctcgct gctggccgcc gtcacgcccg
    15901 ccgagagcaa tgcgctgtac ttcgtctccc gcggcgacgg caccagccag ttctccacca
    15961 accttgccga tcacaatcgc gcagtgaata aatatcagcg ttcccggcga taatgcagtt
    16021 tcaccgggaa gctgaaccat gaaatctggc aagttcatca ccttcgaggg catcgacggc
    16081 gccggcaagt cgacccatgt cgcggccgtc tccgagcaac tgcgcgcgct cggcaagacc
    16141 gtcgtgctga cgcgcgagcc gggcggcacg ccgctgggcg aaaggctgcg cgaactgctg
    16201 ctgcacgagt ccatgcacct cgaaaccgag gcgctgctga tgttcgcgtc gcggcgcgaa
    16261 catctggcgc aggtgatcga acccgcgctt gcgcgcggtg actgggtgat ctcggaccgc
    16321 ttcaccgacg catccttcgc gtaccagggc ggcggacgca agcttccgct cgacaagctg
    16381 cgcgtgctgg agcaatgggt gcatccgcat ttgcagccgg atctcacgct gctgttcgat
    16441 gtgccgatcg acgtcgcgcg cgcgcgcctt gacggctcgc gtacgctcga caagttcgaa
    16501 caggagaagg ccgatttctt cggcgccgtg cgggccggct acctgcagcg cgcggccgat
    16561 tttccggagc gctttcgcgt gatcgattcc acccggccgc ttgaaacggt gcgcgaggaa
    16621 gtgcgcggcg tgattgctgc ccttgcaaca tgaacgactt ttttccctgg cacgaggccg
    16681 cgtggcgcca gttgcagcag atgctcggcc gcctgcccca tgcgatcctg ctgcacggac
    16741 cgcagggcac cggcaagacc gccttcgccg aagcgtttgc ccagtcgctg ctgtgcacga
    16801 accgccacca ggacggccga gcctgcggcg catgcgaggc ctgcggctgg ttcgcgcagt
    16861 acgcgcatcc cgactatcgc cgcgtgcggc ccgaagcgct ggacgacgaa ccgggcgaag
    16921 gcggcgaggg tgaggacggc ggcgacggcg atacgaagaa gtctgcgaag agcaagacgc
    16981 cgtcgaagga aatcaagatc gaccagatcc gcgcgctcgg cgatttcatg aacgtttcga
    17041 cgcaccggca gggcctgcgc gtggtattgc tgtatccggc cgaggcgctc aacacgatgg
    17101 cggccaatgc gctgctgaag acgctggagg aaccgccacc tgcgacggtg ttcatcctgg
    17161 tttcgcacgc gatcgagagg cttttgccga ccatcctgtc gcgctgccgc aagttcgcgc
    17221 tgccgatgcc gccgcgcgcg caggcgctgg cctggctgaa ggaacagggc gtggccgatg
    17281 cggatgactg gctggcggag cagggcggtg cgccgctttc ggccatcgcg gcgtcggcgt
    17341 cggcggttcg cgaacttcag gacgaattcc tgcgcgcgct ggcgcgcccg gacacggagc
    17401 tggccttgaa gacggccgaa cgcctgcaga aggcgccggt atccgatctg gtgttgtggc
    17461 aacagcgctg gctttacgat gtgctggcgc acaaactttc gggcacaatc cggtattatc
    17521 cgcgttaccg cagggaactg gagtcactct cccggcgggc gcccgccgcc gcgctgctcg
    17581 cggcggtcaa gcgcgcgaat gagcggcgcg cggttgccga gcatcctttg gctgcgcggc
    17641 tcttcatcga agacctgctg ctcgactaca ccgcagtgtt tgcatcctga tcacattccg
    17701 aggaccgcat gcccgatccg aacacgatct cgccttccgc ttccggagct gccggcgccg
    17761 tgcgtccttc cgtgctgtcg ctgccgatca aggaaaaggc cgcgctgtac gcggcctaca
    17821 tgccgtatct cgccaacggc ggcatcttcg tgccgaccaa caagccatac aagatcggcg
    17881 acgagatcta tctgatcctc acgttgatgg acgatccgac caaatacccg atcgccggca
    17941 agatcgcctg gatcacgccg gccggcgcca acaacaacaa ggcacagggc atcggcgtgc
    18001 acttctcgtc ggacgagagc gggcagcgtg tgaagctgcg catcgaggaa ttgctcggcg
    18061 cggcgctgcg ctcgactcga gcaacgcata ccctctgatc ttccgtcccg ttcctgtttc
    18121 atgtcccgcc acatggcttt ctctcaccgc cttgcgcgct tcgaagacct gcccgccatt
    18181 gtcgcgatct acaacagcac cgtagcctcg cgcgaagtca ctgccgatac cgaaccgata
    18241 accgtcgagt cgcgccacgc gtggttcgcc gagcacacgc ctgaccggcg gccattgtgg
    18301 gtggcggaac aggatgggga gatcatcggc tggctgtcgt attcgaactt ctacggccgc
    18361 cccgcttatg ccgggacctc cgagctgtca atctatattg ccgagaatgc gcgcggcaag
    18421 gggctgggcc ggtatttcct cgaacaggcg atcgcgcacg cgccgaagat ccaggtgcat
    18481 acgctgctgg gtttcatctt tggccacaac atgccgagcc tcgcgctgtt cgagcgcttc
    18541 ggcttcgaac gctgggcgca tctgccgcgg gtggcgacgc ttgacggcat cgagcgcgac
    18601 ctggtcattc tcggcaagcg cgtcgcctga ctgcgaccgg cgggtgcagc gcgtaaaatg
    18661 cgtacatgta catcgattcc cactgccaca tcaacttccc cgaactcgcc gggcgcctgc
    18721 ccgagatttt cgcgcgcatg gccgacaagc aggttaccca tgcgctgtgc gtgtcggtga
    18781 acctgcccga gtttccgcaa gtcctcgcgc tcgccgagca gcatcccaac atattcgcct
    18841 cggtcggtgt gcatcctgat tacgaagatt cgcccgaacc cacggtcgag gagctggtac
    18901 gcctgtccgg gcatccgaag atcgtggcca tcggcgagac cgggctggac tactaccgtc
    18961 tcgaaggcga tctcgaatgg cagcgcgagc ggttccgcac ccatatccgc gcgtcgcgcg
    19021 cgacgggcaa gccgctgatc atccatacgc gcgcagcgtc ggaggacacc atccgcatca
    19081 tgcgcgagga aggtgccggc acggacgccg gaggcgcggg cggcgtcatg cattgcttca
    19141 ccgaatcgct ggaggtcgcg caggcggcga tggaaatggg gttctatatc tcgttctctg
    19201 gcatcgtcac cttcaagagt gcgcgcgagc tgcaggcggt cgcgcaggcg gtgccgctgg
    19261 agcggatgct gattgagacc gactcgccgt atctcgcccc cgtgccgtat cgcggcaaga
    19321 ccaacgagcc ggggttcgtc gctcacgtcg gtgagttcct tgcaacgctc aaggatgtgc
    19381 cggtcgagca gctcgcgcgc cagaccagcg cgaatttctt caacctgttc aagacagcga
    19441 aaagcctatg agtgcgaccg ccgcggattc cagtgccgcc atgcgccgac gcatgctggt
    19501 ggcggggctt gcctgcatgc tgggcgtcgt cgcgccgccc gcggcgttcg cccagagttc
    19561 ctacgtcgcg tacttccgcg cggtgaagat cgacaatacg cgcacggtcg gctctttgct
    19621 cgcgcgcggt ttcgatccga acacgatcga gcccgatcgc ggcgagaccg gcatgatgct
    19681 ggcgctgcgt gaaggcgcgc cgggcgtgtt ccggctgctg ctggaacacc cggagaccaa
    19741 tgtgaatgtg cgcgccttca acggcgacag cgcgctgatg attgcctgct acaccggcaa
    19801 cggcgaagcc gcgcgtgcac tgatcgagcg cggcgccgcc gtgcaccatc ccggctggac
    19861 gccattgcac tatgctgccg ctggcggcca taacgacatc gcgcagatgc tgatggcgct
    19921 gggcgcgccg ccggatgcgc gctcgccgaa caacaccacg ccgatgatga tggcggcgct
    19981 gggcggacac atcatgaccg tcaaggtgct gctggatgcc ggggccgacg cgacgctgaa
    20041 aaacgatcac ggcatgaccg cgatcgattt cgcgcgccgc aacgaccacc aggacatcgt
    20101 cgacgggctg atctggcggc tcagacgggc cgggaaactg taggcagctc gaggcgcgcc
    20161 tccagtcccc cgccctcgca attggacagc gcgacgctgc cgccatgctg ctcggcgatg
    20221 ttgcgggcaa tcgtcaggcc aagtccggtt ccgccagtat cacgcg
//
LOCUS       DATLGF010000164         6118 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_67316,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000164 DATLGF010000000
VERSION     DATLGF010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67316"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..100)
                     /locus_tag="VM406_05035"
     CDS             complement(<1..100)
                     /locus_tag="VM406_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HVL75360.1"
                     /translation="MNCPLRKRWTRRKEARPQELLDAALDVFVERGY"
     gene            complement(534..1472)
                     /locus_tag="VM406_05040"
     CDS             complement(534..1472)
                     /locus_tag="VM406_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVL75361.1"
                     /translation="MAGLPDHLDIHLIRILYLLLSEKNVSRVALKLNQPQPSISAALR
                     KLRDLTGDPLLVRGARGMVPTQHGESLLKPAKHILDETERLFAHKTAFAPQQEARTFR
                     IAAPDYLDSQFLPNIVAGVRRESPKSRVVIHSLGPEIDYLRHLSDGELDLVIANWEEP
                     PQHLHMSKLFEDEIACIMRADCAYALRTGEDEMTLEDYLALPHVAPSQMLAGYSGVIE
                     SFLAKQGLHRNVVIESAYFGLIPYMLTRTDLVLTTGRQFMKFYEKSLPLKTFRVPVNF
                     PAMRFYQLWHERVHHAPEHKWLRDLVASAAGALVSR"
     gene            1714..2940
                     /locus_tag="VM406_05045"
     CDS             1714..2940
                     /locus_tag="VM406_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HVL75362.1"
                     /translation="MEALLLNYGVEWLNLLLRWLHLITGIAWIGASFYFVWLDNSIKP
                     PKPGSDLEKAGVSGELWAVHGGGFYNPQKYLVAPKQLPPELHWFKWEAYWTWLSGFAL
                     LWVVYYFNPSAMMIDRSVMDLAPWQAVAIGLGSLVVGWTVYDLLCRSPLGQREGLLAI
                     VIFVLLVASSWGLGQMLGGRAAYIHVGAMIGTIMVANVLMVIIPGQRKMVDAMKAGGM
                     PDPVYGKRAKQRSVHNNYFTLPVLFIMISNHYAMTYNHPHNWAVLALIMAAGVLIRHF
                     FNLKHKGIYAWQYPAAGVALLIATAVWIAPSPVQRAAPVAGADAPAAAVDTARIREVV
                     MQRCAVCHSAQPTQPGFASAPAGVMLHTEDLMRQHAERIYRTTVQTRSMPIGNLTNMT
                     EEERALIAQWFQAGAR"
     gene            2965..4596
                     /locus_tag="VM406_05050"
     CDS             2965..4596
                     /locus_tag="VM406_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HVL75363.1"
                     /translation="MNTIQIDGFNLTAEQVVSIARNPDVKVELAQSSRDALKQSRDYI
                     ESTWMHDEAPMMYSFNTGVGLLKDTRIKVEHIELFQTQMIKAHAAGLGEPFSEEVSRA
                     TMLLRANAFASNYSAPRVEVVDRLLAFVNAGIHPIMPQKGSVGASGDLAPLAYLGAAI
                     AGFDEAEVMYQGRRMKAVEAIAAAGIGPVKFDLKAKDASALINGCTVSLAVAVLAARD
                     ARDLLADACVSLGLTLEAMRAEMAGFDERIHLARPHPGQIKTAAIVRKLLEGSTRTTH
                     EARAVQFPDELRRSDVPYTPRIQDVYSLRCAPQVYGPVFDALDYIDNIVAKEINSATD
                     NPLIFSKEGGGFEIISGGNFHGQYLAQAMDLLAMAITDLGSICERRIARLIDPTLSWG
                     LPRNLMTGIRGVNTGYPVVQCSMSALVMENRTLCMPGSVDSIPAKGNSEDHISNSTWC
                     ARKAATVIANAQYIIGVEMLLAAQGLTMTEDLLPGFVLGRGTQAAYDEIRRQIPACLD
                     GDRWFHDDIAAARAFVTSGSVRGAVEKEVGEFMRG"
     gene            complement(4793..>6118)
                     /locus_tag="VM406_05055"
     CDS             complement(4793..>6118)
                     /locus_tag="VM406_05055"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HVL75364.1"
                     /translation="GHAETLRAIDAANAAWLAWRELTGKDRAIILRRWFDLLIANADD
                     LAALMTAEQGKPLAEAKGEVVYGASFIEWFAEEAKRVSGDVMASTWSDKRMVVLKQPV
                     GVCAAITPWNFPIAMITRKVAPAIAAGCTIVIKPAEQTPLSALAMAELAQRAGFPPGV
                     INVVTADAQRSVEIGKALCESPIVRHLSFTGSTPVGRILMQQCAPTVKKLALELGGHA
                     PFIVFEDADLDAAVEGALISKYRNAGQTCVCTNRFYVHESVYDAFIEKLAAGAARIKV
                     GSGFEQGVAQGPLIDDQAVAKVEEHVADAVAKGAKVLAGGKLHALGGTFYEPTVLGNV
                     SADMKVMREETFGPVAAVAKFSTEEEAIAAANDTEFGLAAYFYSRDVGRVWRVGEKLE
                     YGMVGINTGLISNEVAPFGGVKQSGLGREGSKYGIDEYLEMKYLCIGGI"
BASE COUNT         1077 a         1960 c         1978 g         1103 t
ORIGIN      
        1 catagccgcg ttcgacgaac acgtcgagcg ccgcgtccag caactcctgc ggacgcgcct
       61 ccttgcggcg cgtccagcgt tttctcagag ggcaattcat cgacatccga ataactaact
      121 tttgagtcag tagtgtgagc gggaaatgga ggcgcgtcaa gcggagccgg gggcggcgat
      181 gacgtgacat gatggtacgt tgcgcggcca ggagccgcgg cctgtgggaa tgccttcact
      241 taagcccatt cctttcgctc cccgaggcgg gctcccgctc aggcgggagc ctaggggacg
      301 cagtgacgcg gcctgaagcg gcgcagctgg gtccccgcct gcgcggggac gacattgcgc
      361 ggatggtggc tcgtcccgga tatcttcgcg atgacacgcg cggggctgct tgcaaatgcg
      421 atgcccctgc attgtcgctc ccctaggcgt gccccgctca ggcgggagcc cagatgatgc
      481 cgtgacgcgc tcgatgcggc ggagctggac tgccgaagcc gagacggcct cctctacctc
      541 gacaccagcg cacccgcagc cgacgccacc aggtcccgca gccacttgtg ctccggcgca
      601 tggtgcacgc gttcatgcca aagctggtaa aagcgcatgg ccgggaagtt caccggcacg
      661 cggaaggtct tcagcggcag gctcttctcg tagaacttca tgaactgccg gccggtggtg
      721 agaacaaggt cggtgcgggt gagcatgtag gggatcagcc cgaaatatgc cgattcgatc
      781 accacgttgc ggtgcaggcc ttgcttggcg aggaaggact cgattacgcc ggaatagccg
      841 gccagcatct gcgagggcgc cacatgcggc agcgccagat agtcctccag cgtcatttca
      901 tcttcgccgg tgcgcagcgc atacgcgcag tccgcccgca tgatgcaggc gatctcgtcc
      961 tcgaacagct tggacatgtg caggtgctgc ggcggctctt cccagttggc gatgacgaga
     1021 tcgagttcgc cgtcggacag gtggcgcaga tagtcgatct cggggccgag gctgtggatc
     1081 accacgcggc tcttcggcga ttcgcgccgc acgccggcga cgatgttcgg caggaactgg
     1141 ctgtcgagat aatcgggcgc ggcgatgcgg aaggtgcgtg cttcctgctg cggcgcgaag
     1201 gccgtcttgt gcgcgaacag gcgctcggtt tcgtcgagga tgtgcttggc gggcttgagc
     1261 aggctttcac catgctgggt cggcaccatg ccgcgcgcgc cgcgcaccag cagcggatcg
     1321 ccggtcagat cgcgcaactt gcgcaatgcc gccgagatcg aaggctgggg ttgattgagc
     1381 ttcagtgcga cgcgggaaac gttcttctcg gaaaggagca ggtagagaat tcggataaga
     1441 tgaatgtcga gatggtccgg caaacccgcc atgcgatgcc tctatatggg gttgggtatg
     1501 tgaaatatac taggtgccca atgatgcatg ggaaaaaaga gtctatcttg taatactctt
     1561 gccagcatca gcctgcgcgc atgaagggta tgtgccgcac ggcattcaac aggtcgcagc
     1621 ggagctttca acagttcgcg gccaaccggg agtgaatccc tttctcgtct ggagcgcccg
     1681 cggtggcaga cgagcttcaa tcatgaggaa tcgatggaag ctttgctgct caactatggc
     1741 gtggaatggc tcaatctgct gctgcgctgg ctgcacctga tcaccggtat cgcctggatc
     1801 ggcgcttcgt tctacttcgt ctggctcgac aactcgatca agccccccaa gcccggctcg
     1861 gacctggaaa aggccggcgt ctccggtgag ctctgggccg tgcatggcgg cggcttctac
     1921 aacccgcaga aataccttgt cgcgcccaag cagttgccgc cggagctgca ctggttcaag
     1981 tgggaggctt actggacctg gctgtccggc ttcgcgctgc tgtgggtcgt ctactacttc
     2041 aatccctcgg caatgatgat cgaccgctcg gtcatggatc tcgccccgtg gcaggcggtg
     2101 gcgatcggcc tgggctcgct ggtcgtcggc tggaccgtct acgatctgct gtgccgctcg
     2161 ccgctcggac agcgcgaagg gctgctggcg atcgtgatct tcgtgctgct ggtggcgtcg
     2221 tcctggggtc tcgggcagat gctcggcggc cgcgcggcct acatccatgt cggcgcgatg
     2281 atcggcacca tcatggtcgc caacgtactg atggtgatca ttcccggcca gcgcaagatg
     2341 gtcgatgcga tgaaggccgg cggcatgccg gacccggtct acggcaagcg cgccaagcag
     2401 cgcagcgtgc acaacaatta cttcacgctg ccggtgctgt tcatcatgat cagcaatcac
     2461 tacgcgatga cctacaacca tccgcacaac tgggcggtgc tggcgctgat catggccgcg
     2521 ggggtactga tccgccattt cttcaatctc aagcacaagg gcatctatgc gtggcagtat
     2581 ccggccgccg gcgtggcgct gctgatcgcc accgcggtgt ggatcgcgcc gtcgccggtc
     2641 cagcgggcgg ccccggttgc aggtgccgat gcgccagcgg cggccgtgga caccgcccgg
     2701 atccgggaag tcgtgatgca gcgctgcgcg gtctgccatt ccgcccagcc gacccagccg
     2761 ggctttgcca gcgcgccggc cggcgtaatg ctgcacaccg aagacctgat gcgccagcat
     2821 gcggagcgca tctatcgcac cacggtccag acccggtcca tgccaatcgg caaccttacc
     2881 aacatgaccg aggaagagcg cgcgctgatt gcgcaatggt tccaggcagg ggcccgctag
     2941 ccgaaatata gagagagcac atccatgaac accattcaga tcgacggctt caacctcacc
     3001 gctgaacagg tcgtcagcat cgcccggaat cccgacgtca aggtcgagct cgcccagtcc
     3061 tcgcgcgatg cgctcaagca aagccgcgac tatatcgagt ccacctggat gcacgatgaa
     3121 gcgccgatga tgtacagctt caatactggc gtcggcctgc tgaaggacac gcgcatcaag
     3181 gtcgagcaca tcgagctgtt ccagacgcag atgatcaagg cgcatgcggc ggggctgggc
     3241 gaaccgtttt cggaggaagt cagccgcgcc acgatgctgc tgcgcgcgaa tgcctttgcc
     3301 agtaactatt cggcgccgcg tgtcgaagtg gtcgaccgcc tgctggcctt cgtgaacgcc
     3361 ggcattcacc cgatcatgcc gcagaagggt tcggtcggcg cgtccggcga cctcgcgccg
     3421 ctggcctacc tgggcgccgc gatcgccggt ttcgacgaag cggaagtgat gtaccagggg
     3481 cggcgcatga aggcggtcga ggcgatcgcc gccgccggta tcggcccggt caagttcgac
     3541 ctgaaggcca aggatgcgtc ggcgctgatc aacggctgca ccgtctcgct cgcggtcgcg
     3601 gtactggccg cgcgcgacgc gcgcgacctg ctggccgacg cctgcgtgtc gctcggcctg
     3661 acgctggaag cgatgcgcgc ggagatggcg ggattcgacg agcgcatcca cctcgcgcgc
     3721 ccgcatcccg gccagatcaa gaccgccgcc atcgtgcgca agctgctcga aggctcgacc
     3781 cgcaccacgc atgaggcacg cgccgtgcag ttcccggatg aactgcgtcg cagcgacgtt
     3841 ccatacaccc cgcgcatcca ggacgtctat tcgctgcgct gcgcgccgca ggtctacgga
     3901 ccggtgttcg atgcgctgga ctacatcgac aacatcgtcg ccaaggaaat caattccgcg
     3961 accgacaatc cgctgatctt ctcgaaagaa ggcggcggtt tcgagatcat ctccggcggc
     4021 aatttccacg gacagtacct ggcgcaggca atggatctgc tggcgatggc gatcaccgac
     4081 ctgggcagca tctgcgagcg ccgtatcgcc cgcctgatcg accccacgct ctcgtgggga
     4141 ttgccgcgca acctgatgac cggcatacgc ggcgtgaaca ccggttatcc ggtcgtgcaa
     4201 tgctcgatga gtgcgctggt gatggaaaac cgcacgctat gcatgcccgg cagcgtggac
     4261 agcatcccgg ccaagggcaa cagcgaggat cacatctcca actccacctg gtgcgcacgc
     4321 aaggcggcca cggtgatcgc caatgcgcag tacatcatcg gcgtggaaat gctgctcgcc
     4381 gcacagggcc tgaccatgac cgaggatctg ctgccgggct tcgtgctcgg gcgcggcacg
     4441 caggccgcgt acgacgaaat ccggcgccag atcccggcct gcctcgacgg cgaccgctgg
     4501 ttccatgacg acatcgccgc tgcgcgtgct tttgtgacga gcggttcggt gcgtggggcg
     4561 gtggagaagg aggttggaga gttcatgcgc ggctgagctg tgcgctcggc agtgttgcgc
     4621 gcattggatc ggcggtaagc gacgcgctct tgatcgggcg ccatcagcgc atcccctggg
     4681 ctccagcctc cgcgggtgcg acaagcaggg ggcgtcgcat tggcactcag gctggacacg
     4741 tcgagccgac ggcatcggac cgtgccgcca tgccccgcca tgtcggaaga cactagatcc
     4801 ccccgatgca caggtacttc atttcgaggt actcgtcgat cccgtacttc gatccctcgc
     4861 gcccgaggcc ggactgcttg acgccgccga acggcgcgac ttcgttcgag atcaggccgg
     4921 tattgatgcc gaccattccg tactccagct tttcgccgac gcgccatacg cggccgacgt
     4981 cgcgcgaata gaagtatgcg gccaggccga actcggtgtc gttcgccgcg gcgatcgctt
     5041 cttcctcggt gctgaacttc gccaccgcgg caaccgggcc gaaggtttcc tcgcgcatca
     5101 ccttcatgtc ggcgctcaca ttgcccagca cggtaggctc gtaaaaagtg ccgccaagcg
     5161 catgcagctt gccgcccgcg agcaccttcg cgcccttggc gaccgcgtcg gccacatgtt
     5221 cctcgacctt ggccacggct tggtcatcaa tcagcggacc ctgggctacg ccttgctcga
     5281 agccgctgcc caccttgatc ctggcagcgc ccgcggcgag cttctcgatg aacgcgtcgt
     5341 acaccgactc atgcacatag aagcggttcg tgcatacgca ggtctggccg gcattgcggt
     5401 acttcgagat gagcgcgccc tcgacggctg catcgaggtc cgcgtcttcg aacacgatga
     5461 acggcgcatg gccgccaagc tccagcgcga gcttcttcac ggtcggcgcg cattgctgca
     5521 tcaggatgcg gcccaccggc gtggagccgg taaaggacag gtggcgcacg atcgggctct
     5581 cgcacagcgc cttgccgatt tcgaccgaac gctgcgcatc ggcggtgacg acgttgatca
     5641 cgccgggcgg aaagccggcg cgctgcgcca gctccgccat cgccaaagcg gacagcggtg
     5701 tctgctcggc cggcttgatc acgatcgtgc aaccggccgc gatcgccggg gcgaccttac
     5761 gggtgatcat cgcaatcggg aagttccatg gggtgatcgc cgcgcatacg ccgactggct
     5821 gcttcagcac caccatgcgc ttgtcggacc aggtgctggc catcacgtcg ccgctgacgc
     5881 gcttggcttc ttcggcaaac cattcgatga agcttgcgcc gtagacgacc tcgcccttcg
     5941 cttcggcaag cggcttgcct tgctcggcgg tcatcagcgc agcgaggtcg tcggcgttgg
     6001 cgatgagcaa gtcgaaccaa cggcgcagga tgattgcgcg atccttgccg gtcagttcgc
     6061 gccaggccag ccatgccgcg ttggcggcgt cgatggcccg cagcgtttcg gcgtggcc
//
LOCUS       DATLGF010000165         7533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_114792, whole genome shotgun sequence.
ACCESSION   DATLGF010000165 DATLGF010000000
VERSION     DATLGF010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 7533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7533
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114792"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..630)
                     /locus_tag="VM406_05060"
     CDS             complement(19..630)
                     /locus_tag="VM406_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HVL75365.1"
                     /translation="MSATMQTQQQPRKAWDAIAIGASAGGVDAIARLLEALPAHCRAA
                     VFVVLHLAPDSRSVLAQLLARRCALPVKEAEDKELAQPGVVYLCVPDYHLMVEPDRTL
                     ALSRDEPVHYSRPSIDVLFESAALAYRDSLLGIILTGASRDGADGLKQVRACGGTAWV
                     QDPSDAAAPTMPDAALAHAGADAVLPLEKIAARLATFCSQDAA"
     gene            complement(627..1445)
                     /locus_tag="VM406_05065"
     CDS             complement(627..1445)
                     /locus_tag="VM406_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HVL75366.1"
                     /translation="MDSDFDIELKLLLEGVYLKYQHDFRHYAVASLRRRMQQAMARFD
                     CKSLSQLQHRVLHEPEVFAQMLAYFTVQVSEMFRDPSYFKALREHVIPMLRTYPSIKV
                     WIAGCSTGEEAWSLAILLEEEGLLDRTVMYATDINPAALRAAEAGVYDLDRIARFSVN
                     YQQAGGTRSLSDYYQAAYDRAIFRQSLKSQIVFADHSLATDSVFSEMHLVSCRNVLIY
                     FDRELQNRAVKLFYDSLVHRGFLGIGPKENLRFTPFVHAFSEIAPGERIYQRIG"
     gene            complement(1452..4577)
                     /locus_tag="VM406_05070"
     CDS             complement(1452..4577)
                     /locus_tag="VM406_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL75367.1"
                     /translation="MPSQDKLRRTSYFYILGISLLVFLIDCFTPLGYAIWLLYVFPVA
                     LAMLESRPDLPVAIAVGLLPLLLIGYVLSPEGVNETIAVVNRMIAVLAVFGVAYMSRR
                     MISERLVARHLIWIEKGRAEVSRSILGEPTVEELSDRILQALARYFGAQVAAFYRIEG
                     DALVRSGGYALDPARTPREIALGQGLTGEAARSGKPVAVSDLPPDYLHVSSATGTAVP
                     AQVLAAPFTTDGRVSGVLEFGFVKPGDLRSEIELLELVSEKIGSAMRSADYRQHLKNL
                     LEETQRQSEELQAQQEELRVTNEELAEQAKVLQESQAQLESQQSDLEQANVQLEERNQ
                     LLEQQKRNLIQAQLALEKNSAELERANRYKSEFLANMSHELRTPLNSALILANVLAEN
                     KAGTLTDEQVRYARTIHSANTDLLALINDILDLSKIEAGQVEVTVEPVPIERILGALR
                     QTFEPVAREKGLSLRFAASADAPGSMQTDPLRLQQILRNLLSNALKFTSEGEVRLDVS
                     RAPDNRLRFAVVDTGVGIAEDQQEVIFEAFRQADGSTSRTHGGTGLGLSISRQLAQLL
                     GGDVHVSSRPGAGSTFTLEVPCEITVPETGSASVPAALPAPRRAAAEAVPASAPADSL
                     EKRAEPARAKGMTAVEQLADDRDNRSRDRLILVIEDDAKFARILYDLAHEMDFDCLHA
                     ATGAEAIQLAEQFKPSGILLDVGLPDQSGLSVLETIKRNPATRHLPVHVLSVDDYMQS
                     AFELGAVGYALKPVAREQLIEAIGRLERVSQTQVRQVLIVEDNIALRESLEVMLGGED
                     IEITLAGSVAEALEKLSASTFDCMVMDLMLPDGSGYDLLERISEEGKYAFPPVIVYTG
                     RVLSRDEEQRLRRYSRSIIVKGAKSPERLLDEVTLFLHRVESSLPPDQQKLLSQARQR
                     DSAFEGRRILIAEDDVRNVFALSSIFEPLGANLVIARNGKEALAKLEQEPEIDLVLMD
                     LMMPEMDGLTAIRRLREQPRHSRLPVIALTAKAMADDRRDCLAAGASDYIAKPIIVDQ
                     LLSLCRVWMPK"
     gene            4809..6095
                     /locus_tag="VM406_05075"
     CDS             4809..6095
                     /locus_tag="VM406_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVL75368.1"
                     /translation="MKLETLAVHAGYSPDPTTKAVAAPIYQTVAYAFDNAQHGADLFD
                     LKVAGNIYTRIMNPTQDILEKRMAALEGGVGALAVASGMAAITYAVQTICEAGDNIVS
                     ASTLYGGTYNLFAHTLPQMGIETRFADAARPDSFAALIDARTKAIFCESIGNPLGNIT
                     DFAALADIAHAHGIPLIVDNTVPTPYLCRPFEHGADIVVHSLTKYLGGHGNSVGGAIV
                     DSGKFPWAQHKDRFKRLNEPDVSYHGVVYTEALGEAAFIGRARVVPLRNMGAAIAPMN
                     AFLILQGIETLALRMDRICENALKVARYLKSHSKVAWVNYAGLEDHADHALAQKYMAG
                     RASGILSFGLKAADKADPRAAGARVLDALQLFTRLVNIGDAKSLATHPASTTHRQLNP
                     EELAKAGVSEEMLRLSIGIEHVDDLIADLDQALAAA"
     gene            6130..6771
                     /locus_tag="VM406_05080"
     CDS             6130..6771
                     /locus_tag="VM406_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HVL75369.1"
                     /translation="MCANYVPSRYEHLMRHFQVAPPDSPWKEEAYPGYMAPIVRLPRA
                     DALPGDRAAVLGMFGMVPHWADLKLARQTYNARTETVAAKPSFRNAFKRRQYCLIPAE
                     SFYEPSYETGKPVRWEIARADGAPMAIAGIWEYRADGPNGLPLISFSMLTVNADGHPM
                     MQRFHKPNDEKRMVVILDPEQYDAWLHCTPDEAVHYFTRYPSEGLTARPAPRT"
     gene            6783..>7533
                     /locus_tag="VM406_05085"
     CDS             6783..>7533
                     /locus_tag="VM406_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta(1)-pyrroline-2-carboxylate reductase
                     family protein"
                     /protein_id="HVL75370.1"
                     /translation="MPNSALLTPEATAAALPYPRLAQAIASLLADPGVTVPPRLVERL
                     PNGASLFVMPAHDGKVAITKLITFVPDDSERGLPSIQGDIVVFDVATGQRIAILDGPT
                     VTARRTAAVSLLAAQTLAPRTDGPLLIVGAGVQGRSHLEAFRAGLGVDEVWIASRSAT
                     SAQALVAHAQSLGMRARQVQDADAALAECPLVVTATPAQQVVLRARPRDDAFVAAVGA
                     FTPKMVEWAPEVSTHLAATGTLVVDTRDADHE"
BASE COUNT         1301 a         2500 c         2465 g         1267 t
ORIGIN      
        1 tatcggtcat ggcggctttc aggcagcgtc ctgggaacaa aaggtggcaa ggcgggccgc
       61 gattttctca agcggcagca ccgcgtcggc gcctgcatgg gccagcgccg cgtcaggcat
      121 ggtgggggcc gcggcatctg acgggtcctg gacccaggcc gtcccgccgc acgcgcgcac
      181 ctgtttcagc ccatccgcac cgtcgcggct ggcgccggtc aggatgatgc cgagcaggct
      241 gtcgcggtag gcgagggcgg ccgattcgaa cagcacatcg atcgaagggc gcgagtagtg
      301 gaccggctcg tcgcgcgaca atgccagcgt gcggtccggt tcgaccatca ggtgatagtc
      361 gggaacgcac aggtagacga cgccgggctg cgcgagctcc ttgtcctcgg cttccttcac
      421 gggcagtgcg cagcggcggg caagcagctg ggcaagtacg ctgcgcgagt ccggcgctag
      481 gtgcagcacg acgaataccg ccgcgcgaca gtgtgcgggc agggcctcga gcaggcgcgc
      541 aatcgcatcg actccgccgg cggacgcgcc gatggcaatc gcgtcccagg cctttcgggg
      601 ctgctgctgc gtttgcatgg ttgcgctcat cctatcctct gatagatgcg ttcgccgggg
      661 gcgatctccg agaacgcgtg cacgaacggc gtgaaacgga gattttcctt cgggccgata
      721 ccgaggaagc cgcgatggac cagcgaatcg tagaagagct tgaccgcgcg gttctgcagc
      781 tctcggtcga agtagatcag cacattgcgg caggacacca gatgcatctc cgagaatacg
      841 ctgtcggtgg ccaggctgtg gtccgcgaac acgatctgag atttcagcga ctgacggaag
      901 atcgcgcggt catacgcggc ctggtagtaa tccgacagcg atcgcgtccc gcccgcctgc
      961 tggtagttga ccgagaagcg cgcaatgcgg tcgagatcgt agacgcccgc ctcggcagca
     1021 cgcagggcgg cggggttgat gtcggtcgca tacatcaccg tgcgatcgag cagcccctct
     1081 tcctcgagca ggatcgccag cgaccacgct tcctctccgg tgctgcatcc ggcgatccat
     1141 accttgatcg aagggtaggt gcgcagcatc ggtatcacgt gctcgcgcaa ggccttgaaa
     1201 tacgacggat ccctgaacat ctcgcttacc tgcacggtga agtaggcaag catctgcgcg
     1261 aacacctccg gctcgtgcag cacccggtgc tgcagctgag acaagctctt gcaatcgaac
     1321 cgcgccatcg cctgctgcat gcgccggcgc agcgaggcca ccgcgtaatg gcggaaatcg
     1381 tgctggtact tgagatatac cccttccagc agcagcttga gctcgatgtc gaaatcggaa
     1441 tccatgttcg cttatttagg catccatacc cggcacagcg aaagcagctg gtcgacgatg
     1501 atcggcttgg cgatgtagtc gctggcgccg gcggccagac agtccctgcg gtcgtcggcc
     1561 atcgccttgg cggtcagcgc gatcaccggc agccggctgt gcctgggctg ttcacgcaag
     1621 cggcggatcg cggtcaggcc gtccatctcg ggcatcatca ggtccatcag gaccaggtcg
     1681 atttccggct cctgttccag cttcgccagg gcttccttgc cgttgcgggc aatgacgagg
     1741 ttcgcgccga gcggttcgaa gatgcttgag agcgcgaata cgttgcgcac gtcgtcttcg
     1801 gcgatcagaa tgcgccggcc ttcgaatgcg ctgtcgcgct ggcgtgcctg cgacaacagc
     1861 ttctgctgat ccggcgggag gctcgattcc acccggtgca ggaacagcgt cacttcgtcg
     1921 agcaggcgct cgggcgattt tgcccccttg acgatgatcg agcgggaata acgccgcaat
     1981 ctttgctcct cgtcacgcga gagcacccgt ccggtgtaga cgatcaccgg cggaaacgca
     2041 tacttgccct cctcggagat gcgctccagg aggtcgtagc cggagccgtc ggggagcatc
     2101 agatccatca ccatgcagtc gaacgtgctc gcggacaatt tctccagcgc ctcggcaacc
     2161 gagccggcaa gggtgatttc gatatcctcg ccgcccagca tcacctcgag gctctcgcgc
     2221 agcgcgatgt tgtcctcgac aatcagcacc tggcgcacct gcgtctgact cactctctcg
     2281 agccgcccga tcgcctcgat cagctgctcg cgcgcgaccg gcttgagcgc atacccgacc
     2341 gcgccaagct cgaacgcgct ttgcatgtag tcgtccaccg acagcacgtg aacgggaaga
     2401 tggcgggtgg cgggattgcg cttgatggtc tccagcaccg agaggccgga ctggtcgggc
     2461 aggcccacat cgagcaggat gccgctcggc ttgaattgct cggccagttg gatcgcttcg
     2521 gcgccggttg ccgcgtgcag gcagtcgaag tccatttcgt gcgcgaggtc gtagaggatg
     2581 cgggcgaact tggcatcatc ctcgatcacc aggatcaggc ggtcgcgcga cctgttgtcg
     2641 cgatcgtcgg cgagctgctc gaccgccgtc atgcccttgg cgcgcgcggg ctcggcgcgc
     2701 ttttcgaggc tgtccgcggg cgccgaagcg ggaacggctt ccgcggccgc gcgccggggg
     2761 gcggggaggg cggcaggaac ggaggcggag ccggtctcgg gaacggtgat ctcgcagggc
     2821 acttccagcg tgaaggtgct gcctgcgccg gggcggctgc ttacgtgcac atcgcctccc
     2881 agcaactggg caagctggcg ggagatcgac aggccaaggc cggtcccgcc atgggtgcgg
     2941 ctggtgctgc cgtcggcctg ccggaacgct tcgaaaatga cttcctgctg atcctcggca
     3001 atgccgacgc cggtatcgac cacggcaaaa cgcaggcgat tatccggcgc gcgcgacacg
     3061 tcaagacgta cttcgccttc gctggtgaac ttgagcgcat tggaaagcag gttgcgcagg
     3121 atctgctgca ggcgcagcgg atcagtctgc atcgacccgg gcgcatcggc gctcgcggcg
     3181 aagcgcaagg acaagccctt ttccctggcc acaggttcaa acgtctgacg cagtgcgcca
     3241 aggatgcgct cgatcggcac cggctcgacc gtcacttcaa cctggccggc ctcgatcttc
     3301 gacaggtcga ggatgtcatt gatcagtgcg agcaggtcgg tgttggcgga gtggatggtg
     3361 cgggcgtagc gcacctgctc gtcggtgagc gtgccggcct tgttttcggc gagcacgttg
     3421 gccaggatca gcgcactgtt gagcggcgtg cgcagctcgt gcgacatgtt cgcaaggaat
     3481 tcggacttgt agcggttggc gcgctcgagc tctgccgagt tcttttccag cgccagctgg
     3541 gcctggatca ggttgcgctt ctgctgctcc agcagctggt tgcgctcctc gagctgcacg
     3601 ttggcctgtt cgaggtcgct ctgctgcgat tccagctggg cctgcgattc ctgcagcacc
     3661 ttcgcctgct cggccagttc ctcgttcgtc acgcgcagct cttcctgttg cgcctgcaat
     3721 tcctcgctct ggcgctgggt ctcttccagc aggttcttca gatgctggcg gtagtcggcc
     3781 gaacgcatcg cgctgccgat cttctccgac accagttcga gcagttcgat ctcgctgcgc
     3841 aggtcgccgg gcttcacgaa gccgaactcc agcacaccgc tgacgcgccc gtccgtcgtg
     3901 aacggcgcgg ccagcacctg cgccggcacc gccgttccgg tcgccgagct gacatgcaga
     3961 tagtccggcg ggaggtcgga cacggcaacc ggcttgccgg agcgggccgc ctcgccggtc
     4021 aagccctggc cgagtgcgat ctcgcgcggc gtgcgcgcgg ggtccagcgc atagccgccc
     4081 gagcgtacca gcgcatcgcc ttcgatccgg tagaacgcgg ccacctgcgc accgaagtag
     4141 cgcgccagcg cctgcaggat gcggtcggag agttcctcca cggtcggttc gccgagtatg
     4201 ctgcgggata cttccgcgcg ccccttctcg atccagatca ggtggcgcgc caccaggcgt
     4261 tccgagatca tccggcgcga catgtaggcc acgccgaaca ccgccagcac cgcgatcatg
     4321 cggttgacca cggcgatcgt ttcgttcacg ccctcgggcg agagcacata gccgatcagc
     4381 agcagcggca gcagtcccac cgcgatggcg acgggcagat ccggccgcga ctcgagcatc
     4441 gccagcgcga cgggaaacac ataaagcagc cagatcgcgt agccgagcgg cgtgaagcag
     4501 tcgatcagga ataccagcag gctgatgccg agaatgtaga agtagctggt tcgtcgcaac
     4561 ttgtcttgtg aaggcatgga tttattccaa aacggaaggc gggcagggtc cggcaccggc
     4621 gcgcaatgca tgtggacgtg catgtcgatg cacctggcat tgggcggcgc agtaccggcg
     4681 agcgaaccgg cagagtttac cagcaacacg caagacgatc gttcgcatgc accacacgag
     4741 tgcgtccggg caggcgcacc cgattcaggg cacaatggcg cgttctgcaa tgagagactg
     4801 gagcgacgat gaaactcgaa accctcgccg tgcacgccgg ctattcaccc gatccgacca
     4861 cgaaggccgt ggccgcaccg atctaccaga cggtcgccta cgcgttcgac aacgcgcagc
     4921 atggcgcgga tttgttcgac ctgaaggtgg ccggcaacat ctacacccgc atcatgaatc
     4981 cgacccagga catcctggaa aagcgcatgg ccgcgctcga gggcggggta ggggcactgg
     5041 cggtggcctc cggcatggcg gcgatcacgt atgcggtgca gacgatctgc gaggcagggg
     5101 acaacatcgt gtccgcgagc acgctgtacg gcggcaccta caacctgttc gcgcacacat
     5161 tgccgcagat gggcatcgag acgcgctttg cagacgcggc caggccggac agcttcgccg
     5221 cgctgatcga tgcccgcacg aaggcgatct tctgcgaatc gatcggcaac ccgctcggca
     5281 acatcaccga tttcgccgcg ctggccgaca tcgcccacgc gcacggcatt ccgctgatcg
     5341 tcgacaacac cgtgccgacg ccgtacctgt gccggccgtt cgagcatggc gcggatatcg
     5401 tcgtgcattc actgaccaag tatctcggcg gacatggcaa cagcgttggc ggcgcaatcg
     5461 tcgacagcgg caaattcccg tgggcgcagc acaaggaccg cttcaagcgg ctcaacgagc
     5521 cggacgtgtc ctaccacggc gtggtctaca ccgaggcgct cggcgaggcg gccttcatcg
     5581 gccgagcgcg cgtggtcccg ctgcgcaaca tgggcgcggc gatcgcaccg atgaacgcgt
     5641 tcctgatcct gcaaggcatc gaaacccttg cgctgcgcat ggaccgcatc tgcgagaacg
     5701 cgctgaaggt cgcacgctat ctgaaatccc attcgaaggt ggcctgggtc aactacgccg
     5761 gcctggaaga ccatgctgac catgcgctgg cgcagaagta catggccggc cgcgcgtcgg
     5821 gcattctctc gttcggcctg aaggcggcgg acaaggccga cccgcgcgcg gccggcgccc
     5881 gcgtgctgga tgcgctgcag ctgtttacgc ggctggtcaa catcggcgac gcgaagtcgc
     5941 tcgcgacgca tccggcctcg accacgcacc gccagctcaa tcccgaagag cttgccaagg
     6001 ccggcgtgtc cgaggaaatg ctgcgcctgt cgatcggcat cgagcacgtc gacgacctga
     6061 tcgctgatct ggatcaggcg ctggccgcgg catagcgcag cgggcccgac ggtccggtag
     6121 aacggaacca tgtgcgcgaa ctacgtcccg agccgctacg agcacctgat gcgccatttc
     6181 caggtggcgc cgcccgattc cccatggaag gaggaggcct accccggtta catggcgccg
     6241 atcgtgcgcc tgccgcgtgc ggacgcgctg ccgggcgacc gcgcggcggt gctcgggatg
     6301 ttcggcatgg ttccgcactg ggccgacctg aagctcgcac gccagaccta taacgcgcgc
     6361 accgaaacgg tggcggccaa gcccagcttt cgcaatgcgt tcaagcgccg ccagtactgc
     6421 ctgatcccgg ccgaatcctt ctacgaaccc tcgtacgaga ccggcaagcc ggtgcgctgg
     6481 gagatcgcgc gcgccgacgg cgcgccgatg gcgattgccg gcatctggga ataccgtgcc
     6541 gacggcccga acggcctgcc gctgatctcg ttctcgatgc tgaccgtcaa tgccgacggc
     6601 catccgatga tgcagcgctt ccacaagccc aatgacgaga agcgcatggt cgtcatcctc
     6661 gaccccgaac aatacgatgc ctggctgcac tgcacgcccg acgaagccgt gcattacttc
     6721 acccgctatc cgtccgaggg cctgacagcg cgcccggcgc cacgcacctg aacctgaccc
     6781 gcatgccgaa ttccgcactc ctgacgcccg aggcgaccgc cgccgccttg ccctatccgc
     6841 gccttgcgca agccatcgca tccctgctcg ccgatccggg cgtgactgtc cccccgcgtc
     6901 tcgtcgagcg gctccccaat ggcgccagcc tgttcgtgat gccggcccac gacggcaagg
     6961 tcgcgatcac caagctcatc accttcgtgc ccgacgacag cgagcggggg ctgccgagca
     7021 tccagggcga catcgtcgtg ttcgacgtcg cgaccgggca gcgcatcgcg atcctcgacg
     7081 ggccgacggt caccgcgcgc cgtactgcag cggtgtcgct gctcgccgcg cagacgcttg
     7141 cgccgcgcac cgacggccca ttgctgatcg tcggcgccgg cgtgcaggga cgctcccatc
     7201 tggaagcctt ccgcgccggg ctcggggtcg atgaagtctg gatcgcctcg cgcagcgcca
     7261 cgagcgcgca ggcgctggtt gcgcacgcgc agtcgctcgg catgcgcgcg cgccaggtgc
     7321 aggatgccga cgctgcgctc gccgaatgcc cgctggtcgt cacggccacg ccggcgcagc
     7381 aggtcgtgct gcgcgcgcgc ccgcgcgacg atgccttcgt cgcggcggtc ggcgcgttca
     7441 cgccgaagat ggtcgagtgg gcgcccgaag tcagtacgca tctggccgcg acaggaaccc
     7501 tggtggtcga cacgcgcgac gccgaccacg aag
//
LOCUS       DATLGF010000166         9621 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_23222,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000166 DATLGF010000000
VERSION     DATLGF010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 9621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9621
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23222"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            591..1493
                     /gene="galU"
                     /locus_tag="VM406_05090"
     CDS             591..1493
                     /gene="galU"
                     /locus_tag="VM406_05090"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HVL75371.1"
                     /translation="MTKIRKAVFPVAGLGTRFLPVTKASPKEMLPIVDKPLIQYAVEE
                     AAAAGITEMIFITGRGKRSIEDHFDKAYELESELASHGKIKLLKEIQSLLPDNLHYSY
                     VRQSEALGLGHAVLCARPLVGDEPFAVVLADDLIDAEVPVLKQMTDLHAQHGASVVGV
                     QNVPLEHTNQYGIVRPQSMGVRIHKVGDIVEKPQPENAPSTLGVVGRYVFTPEIFDYL
                     ENVTPGTGGEIQLTDAIAAMLARHPVLAYEFRGTRYDCGSKLGYLQASVAYGLKHPAV
                     ASEFGAYLSQLQLDSFRQVDSKSA"
     gene            1686..4415
                     /locus_tag="VM406_05095"
     CDS             1686..4415
                     /locus_tag="VM406_05095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HVL75372.1"
                     /translation="MTSAYASAYPEEDRYRQLIEGCVDAIFLLHEGRIVGANRAAATL
                     VGAPSAELLHGRVLADLLKTPQRAGAPFPLQLDVCPASYERQLVLANNSVADVEITIA
                     PYLDRDSDDGRVIVVRDVSARKRIAAALHQQDQYLRVITDAVPALIAYVDSEQRYRFV
                     NAAYEQWFGLARRVLIGSSMRDVLGAAYAQAQPYVARALAGEQVSYETRIESSEGVRH
                     ALAVYTPDRDENGKVCGFSVMVNDITERKSGERQLRERAHQLRLITDAAPVFIARCDP
                     EYRLTFANRTYLDRFRLSPRDIVGKRIPELVGEPAFGRLREFIDTALAGTPCEHEVKL
                     PAADADQQYYMRMMYVPEFDAHGKVRGLVAAVTDVSRLRETEERLHRREREFKTLVEH
                     SPDIIARIDRQMRHLYVNPSVEKLSGLKASAYIGKTKAELGLPESVVRAWDNATRAAF
                     DTGSEQRLLFEYEQEGQIRYFSGRVVPEIDASGQIESVVVIAYDVTERARMEKERDEL
                     LARERSARIQAETAARARDEFLAIVSHELRAPLNGIQSWAHVLENYVKDATSAPLAQR
                     ALQGIKTGVGQQVRLIEDLLDVTRMMSGKLRLVRQPLALLPTLQAAVESVRPLAAAKR
                     IALTCTYRITTEQIEGDADRVQQIFWNLLSNAIKFTHEGGNVWMAATHSEREICVSVR
                     DDGVGISPEFLPHLFDRFSQADTSSTRNHSGLGLGLFLVRHLVELHEGRVTAESRGEG
                     TGTTFTVHFPLRARSQKYAAVAPYEESGVGALPLPSLDGLQVLLIDDQEEARESLTVV
                     LSSAGAQVYAASSGAEAVQWLEGRAEDAMPDVLVCDIAMPGEDGYAVLRRLRTWTNAA
                     GKVPLARMPALALTAFAHREDRIRALTAGFQMHVTKPVAAEELIVVIDTMVTR"
     gene            complement(4478..5881)
                     /locus_tag="VM406_05100"
     CDS             complement(4478..5881)
                     /locus_tag="VM406_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HVL75373.1"
                     /translation="MPHVLIVDDDVNTREALAEIAAGEGFTAAMAGTIRDAQLQIMRQ
                     RPDVVLIDIKLPDGSGMDLFNDIESRATTEIVLITGHASVETAVEALRLGAADYLTKP
                     INFRRLKGLLSRIPRTVDLKEEIGVLRSELRRLGHFGQMLGASPPMQKLYNHIARVAP
                     TEATVLLLGESGTGKELAAQTIHELSLRQKHPFLPVNCGAISPQLIESEIFGHEKGSF
                     TGADRQHKGYFERANGGTLFLDEITEMPIELQVKLLRVLETGSFMRIGGNQEIDTDVR
                     VIAASNRDPEQAVADGKLRLDLYHRLNVFPLRIPPLRERGEDIILLAQHFLDELNKAH
                     GTNKELSPEGSSSLLNYHWPGNVRELRNYMQRAFILSDDVIDASALAPVLTTQSPVGL
                     TLAIPVGTSLADVDRKLIYATLDLCGGVKKRAADILGISLKTLYNRLEEYGPYEGPQQ
                     QQKKDDAAMRGGSDAYH"
     gene            complement(6180..7610)
                     /locus_tag="VM406_05105"
     CDS             complement(6180..7610)
                     /locus_tag="VM406_05105"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HVL75374.1"
                     /translation="MKAIVDTSKVVLIGALLGVAVAGCDRTATDQATDTGATGSPPPA
                     TTGTEAAGTAPDTAAGEPGAAGTDAATDTPAGTAEPGTPGATDPATGTPGTAPEGAAP
                     PATSGAGATGETAAGTGAAGTGEPQSYIGDPAAQGTQGTQGATAGPAVTGTTSDQPAG
                     AAGTVEGQTGVTGGDAASGASGATGATGTGGETAAAGAATPGDTGTGAAGAGATTSGA
                     GSTLGADTASGTGGAAIPAPGSGDVAGAAASGSGTMDSPGGAGVGAQTGAGGDTVTPG
                     ATAGVPLTTGDGGTGASATSAGSNIAGAASPGEQPPLRGDAATTVPGGGTTASGATVG
                     VGAGAAGAPGQPGTAQGMPMDQQPTQQQDQQQGMQQQGMQQQAQQPGQQDTTGAAATG
                     AGAAGVATAGAGATVEDTSLMNRVRAALLAEANVPDENIQVQASGGNVTLTGSVSTSD
                     QAQLAEQVVRQVDGVQNVENNLQTGN"
     gene            complement(7625..7846)
                     /locus_tag="VM406_05110"
     CDS             complement(7625..7846)
                     /locus_tag="VM406_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75375.1"
                     /translation="MRTIKNTSKAFAVMAVLGLALAGCEQQGAGTTGDTGTGGTGAAG
                     GMNPPATTGQDQAGTGGTGGGTGGTGTSN"
     gene            8288..9202
                     /locus_tag="VM406_05115"
     CDS             8288..9202
                     /locus_tag="VM406_05115"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HVL75376.1"
                     /translation="MVGQAPAMVQLRQQIERVAVTDATVLLIGESGTGKEVVANSVHR
                     ASLRSEKPFVAVNCGAIPAHLIEAALFGHEKGSFTGAVRQHIGYFEHASGGTLFLDEI
                     TEMPIEMQVKLLRVLESGSFLRVGGDQEVRVDVRLIAATNRDLEQAVRQGNLREDLMY
                     RLAVFPLRVPPLRERGDDIELLAQHFLDQLNGDAKTKKAFSRTSLDVMRTYSWPGNVR
                     ELKNVVQRAFILSTDTLSIEECISELATKKPTEQDGYLNFFVGTPLADAQREIILATL
                     KHYSGNKRLTAEALGISLKTLYNRLKEY"
     gene            complement(9350..>9621)
                     /locus_tag="VM406_05120"
     CDS             complement(9350..>9621)
                     /locus_tag="VM406_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL75377.1"
                     /translation="IVAFTRSLSQQLAEKGILVNAVAPGPVWTPLIPSSFPEEAVEGF
                     GEKVPLGRAGQPDEIAPSYIFLASADAAYMTGQVLHPNGGEVVNA"
BASE COUNT         1651 a         3199 c         3123 g         1648 t
ORIGIN      
        1 gccgctgaaa gacaaagttc cagcgcaggt gggaaaacac gatgattcct gaagccatgc
       61 ttactccttg tcatacggcg caatcgaagg gttgaggacg gcacgcactt cccgctcgcc
      121 atgccgcagc gctgcagtgc aaaagatgtg ccgcgcgccg gacagcgcgc attggacgga
      181 ggcgggaaaa gcgattaccg gtatcaaggg acagtcttcg ggcgcgccgc attgcggcgc
      241 gtcatggaat gggcgcgcgc cgagcactgc attgcgcacg cggttgaacg acatcccaat
      301 atacggcgcg gatttcgcaa cacgctgatc tggctcaacg gcgctccggc ccaggaccgg
      361 caagctaccg gtacgtttcc tgctacgtac ggaaagacgc cgactgcccg cccgctcgtc
      421 cgcgtgccgc gcacctgcgc gacgcagcac gcaagtgcga gcgggcgtgt cgcgcgtgtc
      481 gccggacatg taacagcatg taaagacgtg cgtgcggctt gtaatcctta cgctgcgcga
      541 ccggcggcag gaacgtaaca gcttcgttca acgcgacaaa gagagaaacc atgaccaaga
      601 ttcgaaaagc ggtgtttccc gtagccggcc tcggcacccg tttcctgccc gtgaccaagg
      661 cctcgccgaa ggagatgctg ccgattgtcg acaagccgct gattcagtat gcggtcgaag
      721 aagccgccgc cgcgggaatt accgaaatga tcttcatcac cgggcgcggc aagcgcagca
      781 tcgaagatca tttcgacaag gcgtacgagc tggaatccga gctcgcctcg catggcaaga
      841 tcaagctgct caaggagatc cagtccctgc tgcccgacaa cctgcactat tcctacgtgc
      901 gccagagcga ggcgctggga ctcgggcacg cggtgctgtg cgcacgcccg ttggtgggcg
      961 acgaaccgtt tgccgtcgtg ctggccgacg acctgatcga cgccgaagtg ccggtcctca
     1021 agcagatgac ggacctgcat gcgcagcacg gcgcctcggt ggtgggtgtg cagaacgttc
     1081 cgctcgagca cacgaaccag tacggcatcg tgcgcccgca atcgatgggc gtgcgcatcc
     1141 acaaggtcgg cgatatcgtc gagaagccgc agcccgagaa cgccccctcg accttgggcg
     1201 tggtcggccg ctacgtgttt acgccggaga ttttcgatta tctggagaac gtcacgccgg
     1261 gcaccggcgg cgagatccag ctcacggacg caatcgctgc gatgctggcc cgccatccgg
     1321 tgctcgccta cgagttccgc ggcacgcgct atgactgcgg ttccaagctc ggctacctgc
     1381 aggcctcggt tgcgtacggc ctgaagcatc cggcggtggc gagcgagttc ggcgcctacc
     1441 tgtcgcagct gcaactcgat agcttccgcc aggtggacag caagagtgcc tgagcctgcc
     1501 aatcatgcgg tccgcatgcg gaccgcttcc gaacaacccg aacgggcgcg gccggccgcc
     1561 ctgcaggcgc ggccgtgcac caaggagcat cgttgccaat ggctgtatcg aacaaaccgg
     1621 cggagcctct cgctgaactg cgtgccgttg ccctgaccgg cacgccgtcg cgaaacgccc
     1681 ctccagtgac ctctgcctat gcatccgctt atccggaaga ggaccggtac cgtcagttga
     1741 tcgaaggctg cgtcgacgcc atcttcctgt tgcacgaagg gcgcatcgtc ggcgcgaacc
     1801 gcgcggcggc cacgctcgtc ggcgccccga gtgccgagct tctgcacggg cgggttctcg
     1861 ccgacctgct caagacgccg cagcgcgcgg gcgcgccgtt cccgctgcag ctcgacgtct
     1921 gcccggcctc ctacgagcgg cagctcgtgc tggcgaacaa cagcgtggcc gatgtcgaaa
     1981 tcacgatcgc tccctacctc gaccgcgaca gcgacgacgg ccgcgtgatc gtcgtgcgcg
     2041 acgtcagtgc gcgcaagcgc atcgccgcgg cactgcacca gcaggaccag tacctgcgcg
     2101 tgatcacgga cgccgttccc gcgctcattg cctacgtcga cagcgaacag cgctaccgct
     2161 tcgtcaacgc cgcctacgag caatggttcg gcctcgcgcg ccgcgtcttg atcggcagca
     2221 gcatgcgcga tgtgcttggc gcggcctatg cgcaggcgca gccgtacgtt gcccgcgcgc
     2281 tcgccggcga gcaggtttcc tatgaaacgc gcatcgaaag cagcgaggga gtgcgccacg
     2341 cactggccgt ctacactccc gaccgcgacg agaacgggaa ggtgtgcgga ttttccgtga
     2401 tggtcaacga catcaccgaa cgcaagagcg gagaaaggca gttgcgcgag cgcgcccacc
     2461 agctgcgcct gatcaccgac gcggcaccgg tcttcatcgc ccgctgtgat ccggaatatc
     2521 ggcttacctt cgccaaccgg acctatctcg accgcttccg cctctcgccg cgcgatatcg
     2581 tcggcaagcg catcccggag ctggtcggag agccggcctt cggccgcctg cgcgaattca
     2641 tcgacaccgc cctggcgggc accccgtgcg agcatgaagt gaaactgccg gccgcggatg
     2701 cggaccaaca gtactacatg cgcatgatgt acgtgcccga gtttgatgcg cacggcaagg
     2761 tgcgcggcct cgtggcggca gtgaccgacg tgtcgcggct gcgcgagacc gaggaacgcc
     2821 tgcatcggcg cgaacgcgaa ttcaagaccc tggtggaaca ctcgcccgac atcattgcgc
     2881 gcatcgatcg ccagatgcgc catctctacg tcaacccgtc ggtggaaaag ctgtccgggc
     2941 tgaaggcgtc cgcctatatc ggcaagacca aggccgaact cgggctgccg gaatcggtgg
     3001 tgcgcgcctg ggacaacgcc acgcgcgcag ccttcgatac gggcagcgag caacgtttgc
     3061 tgttcgaata cgagcaggaa ggccagatcc gctatttctc cgggcgggtc gtgccggaga
     3121 tcgatgccag cggccagatc gaatcggtgg tcgtgattgc ctatgacgtg accgagcgtg
     3181 cacgcatgga gaaagagcgt gacgagctgc tcgcgcgcga acgcagcgcg cgcatccagg
     3241 ccgagaccgc cgcgcgcgcg cgtgacgaat tcctcgcgat cgtctcgcat gagctgcgcg
     3301 cgccgctcaa cggcatccag agctgggcgc atgtgctgga aaactatgtg aaggatgcga
     3361 cctcggcgcc gctcgcgcag cgcgcgctgc aaggcatcaa gaccggcgtg ggccagcagg
     3421 tgcggctgat cgaggacctg ctcgacgtca cgcgcatgat gagcggcaag ctgcgcctgg
     3481 tgcgccagcc gctggcgctc ctgccgacct tgcaggcggc ggtggaaagc gtccgcccgc
     3541 tggcggcggc caagcgcatc gccctcacct gcacctaccg gatcaccacc gagcagatcg
     3601 aaggcgacgc cgaccgggtc cagcagatct tctggaacct gttgtccaac gccatcaagt
     3661 tcacgcacga gggcggcaac gtctggatgg cggccaccca cagcgagcgc gagatctgcg
     3721 tgagcgtgcg cgacgacggc gtcggcatct cgccggaatt cctgccgcac ctgttcgacc
     3781 gcttcagtca ggccgacacc agcagcacgc gcaaccacag cggactcggt ctgggcctgt
     3841 tcctggtgcg ccacctggtc gagctgcatg aggggcgggt gaccgcggaa agccgcggcg
     3901 aaggcaccgg cacaacgttc accgtgcact tcccgctgcg cgcgcgttcg cagaagtacg
     3961 cggcggttgc gccctacgag gaaagcgggg tcggcgcgct gccgctgccc tcgctggatg
     4021 gtctgcaggt gctgctgatc gacgaccagg aggaagcgcg cgaatcgctg accgtcgtgt
     4081 tgtcctcggc cggcgcccag gtttacgcgg cgtcctccgg cgccgaagcg gtgcagtggc
     4141 tggaaggccg cgcggaagac gcgatgcccg atgtgctggt ctgcgacatc gccatgcccg
     4201 gcgaggatgg atacgcggtg ctgcgccggc tgcgcacctg gacgaatgcc gcgggcaagg
     4261 tgccgctggc gcgcatgcct gcgctcgcgc tgactgcgtt cgcgcaccgc gaggaccgca
     4321 tccgcgcgct gaccgcggga ttccagatgc acgtcaccaa gccggtggcg gcggaggaat
     4381 tgatcgtcgt gatcgatacg atggtcacgc gttagcccga gcgcgatcgg cgaacgcact
     4441 gcgcaaagcg ccagccctgg cgcccgccga tcctgcctca gtgataggcg tcgctgccgc
     4501 cgcgcatcgc ggcgtcatcc ttcttctgct gttgctgcgg cccttcatac gggccgtact
     4561 cctcgagccg gttgtacagc gtcttcaggc tgatgccgag gatgtcggcc gcgcgcttct
     4621 tcacgccccc gcacaaatcc agcgtcgcgt agatcagctt gcgatcgacg tcggccagcg
     4681 aggtcccgac cgggatcgcc agcgtaagcc cgaccggcga ctgcgtagtg agcacgggcg
     4741 ccagcgccga cgcatcgatg acgtcgtcgg acaggatgaa cgcacgctgc atgtagttgc
     4801 gcagttcgcg cacgttgccg ggccagtggt agttcaagag cgacgaggag ccctccggcg
     4861 acagctcctt gttcgtgccg tgcgccttgt tcagctcgtc gaggaagtgc tgcgcgagca
     4921 ggatgatgtc ctcgccgcgc tcgcgcagcg gcgggatgcg cagcgggaac acgttgaggc
     4981 ggtgatacag atccaggcgc agcttgccgt cggcaaccgc ctgctcagga tcgcggttgg
     5041 aggcggcgat cacgcgcaca tcggtgtcga tttcctggtt gccgccgatg cgcatgaagg
     5101 atccggtttc cagcacgcgc agcagcttga cctgcagctc gatcggcatc tcggtaattt
     5161 cgtcgaggaa cagcgtgccg ccgttggcgc gctcgaagta gcccttgtgc tgacggtcgg
     5221 cgccggtaaa actgcccttc tcatggccga agatctcgct ctcgatcaac tggggcgaga
     5281 tcgccccaca gttcacggga agaaacggat gcttctggcg cagcgagagc tcgtgtatgg
     5341 tctgcgcggc cagttccttg ccagtgccgc tctcgccgag cagcaggacg gtggcttcgg
     5401 tcggcgccac gcgcgcgatg tggttgtaca gcttctgcat cggcggcgac gcgcccagca
     5461 tctgcccgaa atggccgagg cggcgcagtt cgctgcgcag cacgccgatt tcttccttca
     5521 ggtccaccgt gcgcggaatg cgcgagagca ggcccttcag gcgcctgaag ttgatcggct
     5581 tggtcaggta atcggccgcg ccaagccgca gggcttccac ggccgtctcc acgctcgcgt
     5641 ggcctgtgat gaggacgatc tcggtcgttg cacgcgactc gatgtcgttg aacaggtcca
     5701 tcccgctgcc gtcgggcagc ttgatgtcga ttagcaccac gtccggacgc tggcgcatga
     5761 tctgcagctg cgcatcgcgg atggtgccgg ccatggcggc ggtaaagcct tcgcctgcag
     5821 cgatctcggc cagcgcttcg cgcgtgttga cgtcgtcatc gacaatcagt acatggggca
     5881 tcttgcaatc tcagtccgta ggaaatttgt cttgctgcga ggcagcccga gcgtttcaac
     5941 agcgatcacg cgccgagaaa attcagggcg cgcggttgag cttgcagggg gatgaagact
     6001 gcacgacgac tgcctcggga gctgcggata gaagcaccat ccgcgaatac attcgattgt
     6061 ccggcgcttg ttgtctgcct tattgaccca aaacaggtat cgatgcaaat gcaggatcct
     6121 ggcatgcgaa gactgcattg cagcttcggc atgtcaggat cagaggggtg tgggccgatt
     6181 tagtttccgg tctggagatt gttttcgacg ttttgcacgc cgtccacctg gcgcacgacc
     6241 tgctcggcaa gttgcgcctg gtcagaagtg ctgacgctgc cggtcaacgt gacgttgcca
     6301 cctgacgcct gcacctggat gttttcatcc ggcacatttg cttcggccag caatgccgcg
     6361 cgcacgcggt tcatcaggct tgtatcctcg accgtcgcgc ccgcaccggc agtggcgaca
     6421 cccgctgcgc cggcgccggt cgcagcagcg ccggtggtgt cctgctggcc aggctgctgg
     6481 gcttgctgct gcatgccttg ctgctgcatg ccttgctgct ggtcctgctg ctgggtcggc
     6541 tgctggtcca tcggcatgcc ctgtgcggta cccggctgac ccggcgcacc ggccgcaccg
     6601 gcgcccacgc ctaccgtcgc acccgaagcc gtggtgccac cgcccgggac cgtggttgcc
     6661 gcatcgccgc gcagcggcgg ctgctcgccc ggacttgcgg cgccggcgat gttgctgcct
     6721 gcactggtgg cgctcgcgcc ggtgccgcca tcgccggtgg tcagcggcac gccggctgtc
     6781 gcgcccggcg tcaccgtatc gccacccgcg ccggtctgcg cgccaacccc ggccccgcct
     6841 gggctgtcca tcgtaccgct gccggatgcc gcggcaccgg cgacatcacc ggaaccgggg
     6901 gcggggatgg ccgcgcctcc ggttcctgaa gcagtgtcgg cacccagggt cgaaccggcg
     6961 ccagaggtgg tcgcgcctgc cccggcagct ccggtcccgg tgtcacccgg ggtcgcggct
     7021 cccgcggcgg cggtttcccc gcccgtgccg gtcgcaccgg tcgcaccgga cgcaccgctt
     7081 gccgcatcgc cgccggttac gccggtctga ccctcgaccg tgcccgccgc gccggcaggc
     7141 tggtcgctcg tcgttcccgt caccgccgga cctgcagtgg cgccttgcgt accttgcgtg
     7201 ccttgcgcag caggatcgcc aatgtaggac tgcggttcgc cggttcccgc agcaccggtt
     7261 cccgcggcgg tttcaccggt cgcgccggca ccgctggtgg caggcggcgc cgcgccctcg
     7321 ggtgccgtgc cgggcgtgcc ggtggccgga tcggttgcgc ccggcgtacc gggttcggcg
     7381 gttccggctg gcgtgtcggt ggccgcgtcc gttcccgccg cgcccggctc gccggccgca
     7441 gtgtcgggcg cggtacccgc cgcttcggtt ccggtggttg cggggggcgg cgagccggtc
     7501 gcgccggtat cggtcgcctg gtcggtcgcc gtccgatcac atccagcgac tgccacaccc
     7561 aacaaagcgc ctatgagaac gactttcgaa gtgtcgacaa ttgctttcat cgttttacct
     7621 ttcgctagtt tgaggtgccg gtgccgccgg ttcctccgcc agtgccaccg gtaccagcct
     7681 ggtcttgccc ggtcgtggcc ggtggattca tgccgccggc ggcgccggtt ccgcctgtgc
     7741 cggtatcacc cgtggtaccg gctccttgtt gttcgcaccc agcaagagcc aagccaagga
     7801 cggccatcac cgcaaacgct ttcgaggtgt tcttgatcgt tctcatttct cttcctttca
     7861 taagtcatgg ggttcacgct tgaccggccc atactcggga agttcttgac gaattcacaa
     7921 aacgggccga gcgccggtga ggcagaatcc atgccaatgg cgggaggaaa ttaccggcgg
     7981 cgcagattgc gtcaaacatc gctcgccgca aatgatggcg cgccggcgca gaaaaactta
     8041 cagccgcgaa gtaaggctta cacgtatttt ttccgatacg tggagcggac attccggggc
     8101 gcattgcgcg tccatgcagg cacgctatgc tggacagacg cgccgaacac gtgcgggcgt
     8161 gggccgggaa ctgaattgat gcaatcggct ctgcaagcaa tggttttgac aatcgcgcag
     8221 gcaacgcgcc gtgccgctta ccttcacttc aatgactcag gacaagacaa gcaaacgata
     8281 cgagaccatg gtggggcagg cgcccgccat ggtgcagctt cgccagcaga tcgaacgcgt
     8341 tgcggtcact gacgcaaccg tcctgttgat cggagaaagt ggaaccggca aggaagtcgt
     8401 ggccaactcg gtgcatcgcg caagcctgcg cagcgaaaag ccattcgtcg cggtgaactg
     8461 cggagcgatc cccgcgcacc tgatcgaggc cgcgctgttc gggcacgaga aaggaagctt
     8521 caccggcgcg gtgcgccagc acatcggcta cttcgagcat gcctccggcg gcacgctgtt
     8581 ccttgatgaa atcacggaga tgccgatcga aatgcaggtc aagctgctgc gcgtgctcga
     8641 atcgggcagc ttcctgcgcg tgggtggcga ccaggaggtg cgcgtggacg tgcgcctgat
     8701 cgccgccacc aaccgcgatc tcgaacaggc ggtcaggcag ggcaatctgc gcgaagacct
     8761 gatgtaccgg cttgcggtct tcccgctgcg cgtgccgccg ctgcgcgagc gcggcgacga
     8821 catcgagctg cttgcacagc actttctcga tcagctcaat ggagatgcga agacaaagaa
     8881 ggcgttctcg cgcacctcgc tggacgtgat gcgcacctac tcgtggcccg gcaacgtacg
     8941 tgaactgaag aacgtggtcc agcgcgcgtt catcctctcc accgacacgc tcagcatcga
     9001 ggaatgcatc tccgagctgg cgaccaagaa gccgaccgaa caggacggct acctgaactt
     9061 cttcgtcggc accccgctgg ccgacgcgca gcgcgaaatc atcctcgcca cgctcaagca
     9121 ctacagcggc aacaagcgcc tgactgccga ggcactgggc atcagcctga agaccttgta
     9181 caaccggctg aaggagtact agcggctcgt tccggccgcg gcgcggattt caagcgcgtt
     9241 tcacacggcg aacgacgcaa ccaacgccgt cccgagcgca gcgagggacc tcatcgactc
     9301 atgcgccgga gattcctcgc tgcgctcgga aagacaggtt cattgcgctt cacgcattca
     9361 ccacctcccc cccgttcgga tgcagtacct gccccgtcat gtaggccgca tccgcgctgg
     9421 cgagaaagat gtacgacggc gcgatctcgt ccggctgccc ggcacgtccg agcggcacct
     9481 tctcgccgaa tccctcgacc gcttcctcgg gaaagctgga tggaatcagc ggggtccaga
     9541 ccggccccgg agccaccgcg ttgacgagta tccccttctc tgccagctgc tgcgacagcg
     9601 agcgggtaaa cgcgacgatc g
//
LOCUS       DATLGF010000167         2356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_163432, whole genome shotgun sequence.
ACCESSION   DATLGF010000167 DATLGF010000000
VERSION     DATLGF010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163432"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VM406_05125"
     CDS             <1..325
                     /locus_tag="VM406_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HVL75378.1"
                     /translation="DCDMRLRQEARRGPGAVAETGTLMLLSGPRTWPSASLLPETHVA
                     IVERSRIVAAMEDAFALARAEHGELPRAVNFISGPSRTGDIEQTIVLGAHGPYRVHVI
                     IVDER"
     gene            365..1357
                     /locus_tag="VM406_05130"
     CDS             365..1357
                     /locus_tag="VM406_05130"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HVL75379.1"
                     /translation="MSKPKVLIARSVFPEIVARLAEHFEVDANQEDRLLSSEELIARA
                     RDKDGLYTTPREQVGAALFAACPQLKAVCNMAVGYNNIDLAAASRAGVIVTNTPDVLN
                     ETTADFAWALLLATARRVTESERWLRDGQWKDWRFDAFLGADVHGTTLGIIGMGRIGQ
                     AIARRSIGFNMRVIYHNRSRLPEAQEVAANQARHVSKEELLRSADHVVLVLPYSQATH
                     HTIGAAELDLMKRSGTLVNIARGGIVDDAALVAALREKRIAAAGLDVFESEPKLHPGF
                     LELSNVVLTPHIGSASEPTRRAMANCAADNLIAALSGRMPPNALNPEAFGRAAQ"
     gene            complement(1361..1615)
                     /locus_tag="VM406_05135"
     CDS             complement(1361..1615)
                     /locus_tag="VM406_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HVL75380.1"
                     /translation="MSLQDQFQQAVADSKNLPERPDNMTLLKIYALYKQATEGDVQGE
                     RPGFTDMVGRAKFDAWDALKGTSKDDAMQQYVDLINDLKG"
     gene            complement(1694..2050)
                     /locus_tag="VM406_05140"
     CDS             complement(1694..2050)
                     /locus_tag="VM406_05140"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HVL75381.1"
                     /translation="MSTTMTASGLQYEDITVGSGTEAKAGAYVSVHYTGWLQNADGSA
                     GRKFDSSKDRNDPFAFPLGAGHVIKGWDEGVQGMKVGGVRKLIIPSSLGYGARGAGGV
                     IPPNATLIFEVELLAA"
     gene            complement(2110..>2356)
                     /locus_tag="VM406_05145"
     CDS             complement(2110..>2356)
                     /locus_tag="VM406_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75382.1"
                     /translation="CYAQTRTELIERFGGLTAYSRAPADGLWKPDGGAAVRDELVVVE
                     IMADVLDEAWWKDYRHRLEVRFRQQSILVRALHATLL"
BASE COUNT          381 a          774 c          773 g          428 t
ORIGIN      
        1 cgattgcgac atgcgtctca ggcaggaggc tcgccgaggg ccaggtgcgg tggccgagac
       61 cgggaccctg atgctgctgt ccggcccccg cacctggccc tcggcgagcc tcctgcctga
      121 gacgcatgtc gcaatcgtcg agcgttcgcg catcgtcgcc gcgatggagg atgcattcgc
      181 gctcgcgcgc gccgagcacg gcgagctccc gcgcgcggtg aacttcatct ccggcccttc
      241 ccgtaccggc gacatcgaac agaccatcgt gctcggcgca catggcccgt accgcgtgca
      301 cgtgatcatc gtcgacgagc gctgacgcta caatggccgc tccacctcta cgaggctgat
      361 ggccatgagc aaaccgaagg tcctgatcgc acgctccgtg ttcccggaaa tcgtcgcccg
      421 gcttgccgag catttcgagg tcgacgcaaa ccaggaagac cggctgctct cctccgaaga
      481 actgatcgcg cgcgcacgtg acaaggacgg cctctacacg acgccgcgcg agcaggtcgg
      541 tgcggcgctg ttcgccgcct gtccgcaact gaaggcggtg tgcaacatgg cggtcggcta
      601 caacaacatc gatcttgccg cggccagccg cgccggcgtc atcgtcacca ataccccgga
      661 cgtgctcaac gaaaccaccg cggatttcgc ctgggcgctg ctgctggcaa cggcgcggcg
      721 cgtgaccgaa tccgagcgct ggctgcgcga cggccagtgg aaggactggc gtttcgacgc
      781 cttcctcggc gccgacgtgc acggcacaac gctcggcatc atcggcatgg gacgcatcgg
      841 ccaggcgatt gcgcgccgct cgatcggctt caacatgcgc gtgatctatc acaaccgctc
      901 gcgcctgccc gaagcccagg aggtggcggc caatcaggcg cgtcatgtgt cgaaggaaga
      961 actgctgcgc agcgccgacc acgtggtgct cgtgctgccg tattcgcagg ccacgcacca
     1021 cacgatcggt gcggccgaac tggatctgat gaaacgcagc gggacgctgg tgaacattgc
     1081 gcgcggcggc atcgtggacg atgcggcgct ggtggcggcg ctgcgcgaga agcgcattgc
     1141 cgcggcgggg ctggatgtgt tcgagagcga gccgaagctg cacccgggct tcctggaact
     1201 gtcgaacgtc gtgctcacgc cgcatatcgg cagcgcatcc gaaccgacgc gccgcgcgat
     1261 ggccaattgc gcggccgaca atctgattgc cgccctgtcc ggcaggatgc cgccgaacgc
     1321 cctcaatccg gaagcgttcg gccgggcggc gcagtgagga tcagcccttc aggtcgttga
     1381 tcaggtcgac gtactgctgc atcgcgtcgt ccttcgaggt gcctttgagc gcatcccagg
     1441 cgtcgaactt cgcgcggccg accatgtcgg tgaagccggg acgctcgccc tgcacgtcgc
     1501 cttccgtggc ctgcttgtac agcgcgtaga tcttcagcag cgtcatgttg tccgggcgtt
     1561 ccggcaggtt cttcgagtcg gcgacggctt gttggaactg gtcttgcagg ctcatgctta
     1621 tcccctcggg tgttggtgtt ggtgttgaac gggcggttcg cgctgctccg cccttgttga
     1681 ttttggcgga cgcttaagcg gccagaagtt cgacttcgaa aatcagcgtg gcattgggcg
     1741 ggatcacgcc gccggcgccg cgtgcgccgt agccgagcga ggacgggatg atcagcttgc
     1801 gcacgccgcc caccttcatg ccctgcacgc cttcgtccca gcccttgatc acatggccgg
     1861 cgcccagcgg gaaggcgaac gggtcgttgc gatccttgct cgaatcgaat ttgcggcccg
     1921 cgctgccgtc ggcattctgc agccagccgg tgtagtgcac gctcacatag gcgccggcct
     1981 tggcttcggt gccggagccg acggtgatgt cttcatactg caagccggag gcggtcatcg
     2041 tggtggacat gatttccttt ctggttgggt ctttggcaag gggcggattg tagcagcgat
     2101 gccaggcgct tacagcaggg tggcatggag cgcgcgcact agaattgatt gctggcgaaa
     2161 gcgaacttcg agccgatgcc ggtagtcttt ccaccaggct tcgtccagca cgtctgccat
     2221 gatctcgacg actaccagct cgtcgcgcac cgctgcaccg ccatcaggct tccacagtcc
     2281 gtcggccggt gcccggctgt aggcggtcag accgccgaag cgctcgatca gctccgtgcg
     2341 cgtctgcgcg tagcac
//
LOCUS       DATLGF010000168         9513 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_85676,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000168 DATLGF010000000
VERSION     DATLGF010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 9513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9513
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85676"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..200
                     /locus_tag="VM406_05150"
                     /pseudo
     CDS             1..200
                     /locus_tag="VM406_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011544641.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
     gene            210..1385
                     /locus_tag="VM406_05155"
     CDS             210..1385
                     /locus_tag="VM406_05155"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVL75383.1"
                     /translation="MQLKSLFFGEDTAQALRIKRLLFALNAYFFSIVVVGLAVWQQLA
                     PAVALTCIAAGTVAINVLFYLVIRSGLNRHLADPGMTIPQMALGTLLATLLIYLAPAA
                     RDAFLLGYVLILLFGIFRLKARATILVGAIAVVSYGAVIAAHAASGVLPNPAAEVLQL
                     IMLAFLYPWFAQLGSHLNETRANLRETNNRLAQTLADYERALEQIRLQATHDELTGTY
                     NRRHMMTALQGETRRAGCDGGHFSILVLDIDHFKHVNDRYGHPEGDRVLVEFATLVQE
                     QLRGSDQLGRYGGEEFVVLLPDTGLIAAAAVGERIRSAVAAHAFAGLNEPLTVSIGAA
                     CLQPGETHDQTFLRADRALYRAKALGRNRVEVQDCAATASGAAAAVESPAASSRQAR"
     gene            complement(1307..2296)
                     /locus_tag="VM406_05160"
     CDS             complement(1307..2296)
                     /locus_tag="VM406_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HVL75384.1"
                     /translation="MASEQCGAPLRIMTPIMTECGRHRHRHPQGEHAIDPQFILRDLS
                     AYAQTELGRIFATLIALAVTMALLKLIQRRASRATGTGIEISHRRGNFVLLKNLVLLC
                     ALIVVGAIWASKIAGAALSLAAVAGAMLLVSKEFLTNLLGSAFLTVSRLYRVGDFIEL
                     DGIAGRVIDTDLLATTVSEMQDGSQLTSRTVALPHSLLLVRPVRNLTATGEFVINLLK
                     VGVDASADLLAHEAALLRAAQEVCAPWLEEANDHLKRIERRDLMDLPSAQPRVIFQLN
                     DAKQATLSVRYCCRPNQKVKVEQAILRRYLACRDEAAGDSTAAAAPLAVAAQS"
     gene            2335..2532
                     /locus_tag="VM406_05165"
     CDS             2335..2532
                     /locus_tag="VM406_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75385.1"
                     /translation="MIKVYEYTRQPPYWVVRDTDGYWLVPARRGGWREREPFVGRAGA
                     LREVVDWHAIDPELGQDADGA"
     gene            2634..3353
                     /locus_tag="VM406_05170"
     CDS             2634..3353
                     /locus_tag="VM406_05170"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HVL75386.1"
                     /translation="MSAVSPTAAECPNRLLAALPEPEYQALRPHLEYLVTPIRFIVFE
                     RDKPITHVYFPLEGAHSVLAIMEDGAAVEVGTVGNEGFTTVDVLTGADSAIETTIVQV
                     PGSSLRLPIEQFHAAAAEGSALHRLLTRYLQVYLAQVSQSVACNRLHSIEQRFARWVL
                     MSHDRVHGDRFQLTQEFLADMLGVYRPSVSLVARTFQQAGLIQYQRGVMTILDRGGLE
                     EAVCECYSVVRQRYEKTFGKP"
     gene            3472..3888
                     /locus_tag="VM406_05175"
     CDS             3472..3888
                     /locus_tag="VM406_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75387.1"
                     /translation="MSLAARRVLVAADPRAAERIIPALGPDLAVNIAHSLHAAKAQLD
                     HRPDLIVCALYFDDSRMFDLLRHLKADEATRGIPFIAVKTTEGALSTTLRQGIEIACA
                     ALGAEKFVDYVEWEQQFGAATAREKMRSLARRLRDE"
     gene            complement(3934..4104)
                     /locus_tag="VM406_05180"
     CDS             complement(3934..4104)
                     /locus_tag="VM406_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HVL75388.1"
                     /translation="MNQDQVKGNMKDAAGKVQEKAGEVFNSPEQEAKGIKKQVEGKTQ
                     EKWGDAKDVVDE"
     gene            4343..4675
                     /locus_tag="VM406_05185"
     CDS             4343..4675
                     /locus_tag="VM406_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75389.1"
                     /translation="MAPWIIPALKAVLPHVGTIVAATKPMFTKPEAGEADPAVEQQIA
                     ELQAAAAQNDANIRELAGQLQKTVTALESAAAQAEDKTRRMTQLCWGAMGVSVLALAV
                     AFTALFAS"
     gene            4689..4985
                     /locus_tag="VM406_05190"
     CDS             4689..4985
                     /locus_tag="VM406_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012366648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HVL75390.1"
                     /translation="MSASPARRVRIEPEGFEFDAQESQTLLESARRAGIILPASCRNG
                     TCRTCLCRVASGTIRYAIEWPGVSREEKQEGYVLPCVAHPTSDVVLKVAAARKV"
     gene            5065..6006
                     /locus_tag="VM406_05195"
     CDS             5065..6006
                     /locus_tag="VM406_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVL75391.1"
                     /translation="MKIHQLRYFAEIARSGSFSRAAQTLAIAQPALSQNIAALESELK
                     TKLFDRHAKGVELSPSGRRLYERAIALLDAFDSLKEDVSDAPARPSGRVRICIAGSLA
                     SVVTAPLLSVMLARFPDIDLKISAGMSSDARAQLESGRIHLALMPSAHELDAMTALPL
                     FEEHLKLFGAPCDIARLPEDMSFSALADLPLALPDRAHDLRRIVERAASTLNLSLDVR
                     YELNSPAMLTAVAQSGLACAIMPPSACVDALAAGTLAGRAIVAPELTRVQAVVWPDEH
                     PLSGAAICVRDVICEVVKTLAEAGQLHGRILAPGGAC"
     gene            6123..7586
                     /locus_tag="VM406_05200"
     CDS             6123..7586
                     /locus_tag="VM406_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HVL75392.1"
                     /translation="MKKQNVIVIMSDEHDPRIMGCSGHPFIKTPNLDLLAARGVRFTD
                     AYTPSPICVPARAAFATGMPVHKIRHWDNAMPYAGHQRGWGHVLQDHGVRVESIGKLH
                     YRSEEDPAGFDVEHLPMHVLGGYGMVWGSIRDPFRPRKDGPRMLGERMGPGESSYTQY
                     DRSVTQRAVQWLHDAKSDAGRPFVLYVGLVAPHFPFIVPQEFYDLYPLDTLPEPKLHP
                     STGYKRHPWVQEYADFIGSDDAFADRDERLRAFAAYYGLCSWLDHNVGQILAAVREAG
                     LEDNTQIVYTSDHGDNLGARGVWGKSTLYQESVKVPMLLAGPGVDPGVCATPVDLMDL
                     FPTILEAVGIDPAPEMGDRPGRSLYEVAAAPTDTERTILSQYHAVGSNAAGYMLRKGR
                     WKYHYYVGFAPELFDLEADPEETNDLAGRADHAAVIDSLHQELLKLCDPIAVDRQAKA
                     DQAALIERFGGPDAAHKLGSSSSTPVVLNEEQAQVAP"
     gene            7583..8929
                     /locus_tag="VM406_05205"
     CDS             7583..8929
                     /locus_tag="VM406_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HVL75393.1"
                     /translation="MSDDISALTGPAIEDRFLSDWSGHTEGRPQIVYRPSSTEEVARI
                     VQRCHDAGQRITIQGGMTGVAGGAVPGDGDVVINLERMNRIESIDALEGVMQVQAGAT
                     LQQVQEAAAEQGWMFAVDLGARGSCQIGGNAATNAGGVRVIRYGTMRDSVLGVEAVLA
                     DGSVVSSLTRLVKNSTGLDPRFLFVGTEGTLGIITRLTLRLHPPTGEPATALVAADNF
                     DDLPVLLRALRRDVGSALCAFEFMSGAFMDLATRLTGRTNPLTGRASWYVLVEAAGMP
                     GESMEDRLQAALADALEQGTITDCAVAASLTQAAAFWGLRESIPEILSHLKPVAPLDI
                     GMPWGHTADYLQTVEQELQQRLPDAQHLFLGHLGDNNLHLISGPVDPAGVHAVDEIAY
                     GALRDRNGTVSAEHGVGRLKKEYLHVTRNASEIALMKRLKQALDPKQILNPGRIFD"
     gene            9024..>9513
                     /locus_tag="VM406_05210"
     CDS             9024..>9513
                     /locus_tag="VM406_05210"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVL75394.1"
                     /translation="MNRRHFLATLGVASMSSTSLLSTAWAASYPSSPVRIVVPYAAGG
                     GADGVTRVVAQLLGERLKGSFIVDNRPGAGGVIGAQNVARADADGYTLLMAGNPELTI
                     TPHLLARAPYSVADAFTPIMLVAQSPNVFVGNPAGPRTLAALIEESRKRNAPISVGTP
                     GIG"
BASE COUNT         1701 a         3122 c         3029 g         1661 t
ORIGIN      
        1 ttctaccatc gcggccgcaa gctcggcatc gcgaccggct ggcacggctt cgggcagttc
       61 gatgcggcgg tggagaacgc gcggcgcaag atcgcggcat agcctcggcg ccgcaaacgg
      121 agttgcaggc ctccgacaga acgtcaagtc atgcggcgca agcggcaggt taccggtata
      181 gttatttctt tcggaaatga cccaaaagcg tgcaactcaa gtcgctcttc ttcggtgaag
      241 acaccgccca ggcactgcgc atcaagcgac ttctcttcgc gctgaatgcc tatttcttca
      301 gcattgtcgt cgtcggtctc gctgtctggc agcagctcgc gcccgctgtt gcgctcacat
      361 gcattgcggc cggcacggtc gcgatcaacg tgctgttcta cctggtgatc cgcagcggcc
      421 tgaatcgcca tctcgccgat cccggcatga cgattccgca gatggcgctc ggtaccctgc
      481 tggcgaccct gctcatctac ctggcgcccg ccgcgcgcga cgcctttctg ctcggttacg
      541 tgctgattct gctgttcggc atattccgcc tcaaggcgcg cgcgacgatt ctggtcggcg
      601 ccattgccgt ggtctcttat ggcgcggtca tcgccgcgca cgccgccagc ggcgtgctgc
      661 cgaacccggc cgccgaagtg ctgcaactga tcatgctggc cttcctctat ccatggtttg
      721 cccagctcgg cagccatctg aacgaaacgc gggcgaacct gcgcgagacc aacaaccggc
      781 tcgcgcagac gctcgcggac tacgagcggg cactcgaaca gatccggctc caggccacgc
      841 atgacgagct gactggcacc tacaaccggc gccacatgat gacggcgctg caaggagaaa
      901 cccgccgggc cggatgcgac ggcggccatt tttccatcct ggtgctggac atcgaccact
      961 tcaagcacgt caacgaccgc tacggacatc cggaaggcga ccgcgtgctg gtggagttcg
     1021 cgaccctggt gcaggaacag ctgcgcggca gcgaccagct cggccgctat ggtggagagg
     1081 agttcgtggt gctgctgccg gataccggac tgatcgcggc cgccgcagtc ggcgagcgca
     1141 ttcgcagcgc ggtggcggca catgcgtttg ccgggctgaa cgaaccgctg acggtgtcga
     1201 tcggggcggc gtgcctgcag cccggcgaaa cccatgacca gacgttcctg cgcgccgacc
     1261 gcgcgcttta ccgtgccaag gcgctggggc gcaaccgggt cgaggttcag gactgcgcgg
     1321 ccacggcaag cggcgcggcc gccgccgtcg agtcgcccgc ggcttcgtcg cggcaggcca
     1381 ggtagcggcg caggatcgcc tgctcgacct tcaccttctg gttcgggcgg cagcagtagc
     1441 gtaccgacag cgtggcctgc ttggcatcgt tcagctggaa gatcacgcgc ggctgggcgg
     1501 acggcaggtc catcaggtcg cgccgctcga tgcgcttcag gtggtcattg gcctcttcca
     1561 gccagggtgc gcagacttcc tgcgcggcac gcagcagggc tgcctcgtgc gcaagcagat
     1621 cggccgaagc gtcgaccccc accttgagca gattgatcac aaactcgccg gtagcggtca
     1681 ggttgcgaac cggcctgacc agaagcagcg agtgcggcag cgcaacggta cgactggtaa
     1741 gctggctgcc gtcttgcatt tccgacacgg tagtggcaag caggtcggta tcgatgaccc
     1801 ggccggcaat gccgtcgagc tcgatgaagt cgccgacccg atacaggcgc gagacggtga
     1861 gaaacgccga accgagcagg tttgtcagaa attccttgct taccagcagc attgcacccg
     1921 ccaccgcagc cagcgacaag gccgcgccag ctatcttgga agcccagatc gctccgacga
     1981 cgatcagggc acacagcagg accagattct tgagcaggac aaaattgccg cgccggtgcg
     2041 aaatctcgat gccggtgccg gtcgcgcggc tggcacgccg ctgaatcagc ttgagcagcg
     2101 ccatcgtgac ggccagcgcg atcagggtcg cgaaaatgcg gccgagttcg gtctgggcgt
     2161 aggcgctcag gtcgcgcaga atgaattgcg ggtcgatggc gtgttctcct tgtggatgcc
     2221 ggtgacggtg ccggccgcat tctgtcatga tcggcgtcat gatgcgcagc ggcgcgccgc
     2281 attgctcgct cgccatggcc gttacgacac ttcgcgatcc agggggagat gcggatgatc
     2341 aaggtctacg aatatacccg gcaaccaccg tactgggtgg ttcgcgatac cgacggttac
     2401 tggcttgtcc cggcccgccg cggcggctgg cgcgagcgcg aacccttcgt cggccgcgcc
     2461 ggcgcattgc gcgaagttgt cgattggcat gcgatcgacc ccgagctcgg acaggacgcc
     2521 gacggcgcct gaggcagggc acaactggcg tgaagccctc ctgttccagc gcagaccgaa
     2581 tgtggggaaa cgcggcttaa atggtgagct gactttatct ccatggagct cccatgtccg
     2641 ccgtatctcc gaccgccgca gagtgcccga atcggttgct ggccgcgctt ccggagcccg
     2701 aataccaggc gctgcgcccg catctcgaat atttggtcac accgatccgc ttcatcgtct
     2761 tcgagcgcga caagccgatc acgcatgtgt acttcccgct cgagggggcg cactcggtgc
     2821 tggccatcat ggaagacggc gccgccgtgg aggtcggcac ggtgggaaac gaaggattca
     2881 cgaccgtcga tgtgctgacc ggcgcagatt ccgccatcga aaccacgatc gtgcaggtgc
     2941 ccggcagttc gctgcgcctg ccgatcgagc agttccacgc ggcggctgcg gaaggaagcg
     3001 cgctgcaccg gctcctcact cgctatttgc aggtctatct tgcgcaggta tcgcagtcgg
     3061 tcgcgtgcaa ccggctgcat tcgatcgagc agcgtttcgc gcgctgggtc ctgatgtccc
     3121 atgaccgcgt gcacggcgac cgctttcagc ttacccagga gttccttgcc gacatgctgg
     3181 gcgtctatcg gcccagtgtc agcctggtcg cccgcacctt ccagcaggcc ggcctgatcc
     3241 agtaccagcg cggcgtgatg accattctcg accgcggcgg actcgaggaa gcggtgtgcg
     3301 agtgttattc ggtggtgcgg caacgttacg agaaaacatt cggcaagccc tagggcctcg
     3361 ctggggcctc gctgggcccc attaacaggt ccgctctctg tatgctgcaa tacagacagg
     3421 tgcggcgcgc tcaccgacaa tccgcggcat cttcttcacc ccaggattgt catgtccctt
     3481 gcagcacggc gcgttctcgt cgccgccgac ccacgcgccg ccgagcgcat catccccgcc
     3541 ctcggtcccg acctcgcagt caacatcgcc cactcgctgc atgccgccaa ggcccagctc
     3601 gatcaccggc cggacctgat cgtctgcgcg ctgtacttcg acgacagccg catgttcgac
     3661 ctgctgcgcc acctgaaggc cgacgaagcg acccggggca tccccttcat cgcggtcaag
     3721 accaccgaag gcgcgttgag cacgacgctg cgccagggga tagaaatcgc ctgcgcggcc
     3781 ctcggcgcgg agaaattcgt cgactatgtc gagtgggagc agcagttcgg cgcggcgacg
     3841 gcgcgcgaga aaatgcgcag cctggcccgg cgcctccgcg acgaatgaaa aaacggcggg
     3901 accagcccgc cgtcgatcga aatccggtga cgcttactcg tcgaccacgt ccttggcatc
     3961 gccccatttt tcctgggtct tgccttccac ctgcttcttg atgcccttgg cttcctgctc
     4021 ggggctgttg aatacttcac cggccttttc ctgcaccttg ccggcagcat ccttcatgtt
     4081 gcccttgact tgatcctggt tcataacgac ctcctgtgaa gtaataacgc acgtacaagt
     4141 ttgacgcgcg gggtgaccgt ttgttcaagt tttcgtcagc catgttgtcc tcgcgactta
     4201 gaaccatggc acggcgagga cgggttcgat ccgcggcagc gaccggcccc cgagagccat
     4261 ttgcgtgccg aaacaggcaa cgcttggtat cctgtccctc atgccacaag gccaccctat
     4321 tcaagtttta caggaacaca acatggcgcc ctggatcatc cctgcactga aagccgttct
     4381 gccgcacgtc ggcaccatcg tcgccgccac caagccgatg tttacgaagc cggaagcggg
     4441 agaagccgat ccggcggtcg aacagcagat cgcggaactg caggcggcag ccgcgcagaa
     4501 cgatgcaaac atccgcgagc tggccggtca gttgcagaag acagtcaccg cgctggaaag
     4561 cgcggcggcc caggccgagg acaagacgcg ccgcatgacg cagctgtgct ggggcgcgat
     4621 gggcgtctcg gtcctcgcgc tcgccgtggc cttcaccgcg ctgttcgcca gctaagaccc
     4681 ggcatttcat gtccgcttcg cccgcgcggc gcgtacgaat cgagccagag ggattcgaat
     4741 tcgacgcgca agaatcgcag acccttctgg aatcggctcg ccgcgccggc atcattcttc
     4801 ccgcttcctg ccgcaacggc acctgccgca cctgcctgtg ccgggtggcg agcgggacga
     4861 tccgctatgc aatcgagtgg ccgggcgtga gccgcgagga aaagcaggag gggtatgtgc
     4921 tgccctgcgt cgcgcatccg acgtcggatg tggtgctgaa ggtggcggcg gcgcgcaagg
     4981 tctagtcacg ctacgcgcag gtccttcctg cggctgctcc ggcaactgcc ttatactggt
     5041 cccggccgcc tccactcccc tcccatgaag attcaccagc tcaggtattt cgccgaaatc
     5101 gcccgcagcg gcagtttctc gcgcgcggcc caaactttgg cgattgccca gccggcgttg
     5161 agccagaaca tcgcagcgct tgagtcggag ctcaagacga aactgttcga ccgccatgcc
     5221 aagggcgtcg agctttcacc cagcggacgc cggctttacg aacgcgccat tgcgctgctc
     5281 gacgccttcg actcgctcaa ggaggacgtc tcggacgcac cggcccgccc atccgggcgg
     5341 gtgcgcatat gtatcgccgg ttcactcgca agcgtcgtga cggcgccgct tttgagcgta
     5401 atgctcgcgc gctttccgga catcgacctg aagatcagcg ctggcatgtc cagcgacgcg
     5461 cgcgcccagc tggaatcggg ccgcatccac ctggcgctga tgcccagtgc gcacgagctc
     5521 gatgccatga ccgcgctgcc gctgttcgag gaacacctca agctgttcgg cgcgccgtgc
     5581 gacatcgcac gcctgcccga ggacatgagc ttttctgcgc ttgcggacct gccgctcgcg
     5641 ctgcccgacc gcgcccatga cctgcgccgc atcgtcgaac gcgcggccag caccttgaat
     5701 ctttcactcg atgtgcggta cgagctcaac agcccggcga tgctgacggc agtggcgcag
     5761 agcgggcttg cctgcgcgat catgccgccc agcgcctgcg tggatgcact cgctgccggc
     5821 acgcttgcag gacgcgcgat cgttgcgccg gaacttacgc gcgtccaggc cgttgtgtgg
     5881 cctgacgaac atccattgtc tggcgccgcc atctgtgtgc gagacgtgat ttgcgaagtg
     5941 gtgaagacgc tggcggaagc cgggcaactg cacggccgga ttctcgcccc cggcggcgcg
     6001 tgctgagctc gcatccataa gcaaacgtga tcgctgccca gttccaaact gtattggatt
     6061 tgccactacg cgtatgacag tattttcgaa agccgggcac gcagcccgac agaggagata
     6121 ctgtgaagaa acagaacgtc attgtgatca tgtccgacga gcatgatccg cgcatcatgg
     6181 gttgctccgg ccacccgttc atcaaaaccc ccaacctcga tctcctcgcc gcgcgcggcg
     6241 tgcgctttac cgacgcttac acgccgagcc ccatctgcgt accggcgcgc gccgccttcg
     6301 ccaccgggat gcccgtgcac aagatccgcc attgggacaa tgcgatgcct tacgccgggc
     6361 accagcgcgg ctgggggcat gtgctgcagg accatggcgt gcgcgtcgag agcatcggca
     6421 agctgcacta ccgcagcgag gaggatccgg cgggcttcga cgtcgaacat ctgccaatgc
     6481 atgtgctcgg cggctacggc atggtctggg gctcgatccg cgacccgttc cgtccacgca
     6541 aggatgggcc gcgcatgctg ggcgagcgga tggggccggg agaatcgtcg tacacgcaat
     6601 acgaccgctc ggtgacgcag cgcgcggtgc agtggctgca tgacgcaaaa agcgatgcgg
     6661 ggcgcccgtt cgtcctgtat gtcggcctcg ttgccccgca ttttccgttc atcgtgccgc
     6721 aagagttcta cgacctctat ccgctcgata cgcttcccga gccgaagctc caccctagca
     6781 ccggctacaa gcgccatccg tgggtgcagg aatatgcgga cttcatcggc tcggacgatg
     6841 cgttcgcgga ccgggacgag cgcctgcgcg cgttcgcggc ctactacggc ctttgcagct
     6901 ggctcgacca caacgtcggc cagatcctcg cagccgtgcg cgaagccggc ctggaagaca
     6961 acacccagat cgtctatacg tcggaccatg gcgacaatct cggcgcgcgc ggcgtctggg
     7021 gcaagtcgac gctgtaccag gaaagcgtca aggtgccgat gctgctggcc ggtccgggcg
     7081 tcgatccggg cgtatgtgcg acaccggtgg acctgatgga cctgttccct acgatcctgg
     7141 aggcggtggg catcgatccg gcgccggaaa tgggcgatcg acccggccgt tcgctgtacg
     7201 aagtggcggc cgcaccgacc gatacggagc gcacgatact tagccagtac cacgcggtgg
     7261 gcagcaatgc agccggatac atgctgcgca agggacgctg gaaataccac tactacgtgg
     7321 gcttcgcgcc ggagctgttc gacctggaag ccgatccgga agagaccaat gatcttgccg
     7381 ggcgtgccga ccatgcggcc gtgatcgaca gcctgcacca ggaactgctg aagctctgcg
     7441 atccgatcgc ggtcgaccgc caggccaagg cagatcaggc cgcgctgatc gaacgtttcg
     7501 gcggtccgga cgccgcgcac aagctcgggt cgtcgagttc gacgccggtg gtcctcaatg
     7561 aagaacaggc gcaggtggca ccttgagcga cgacatttcg gcgctgaccg gtccggccat
     7621 cgaggaccgg ttcctttcgg actggagcgg ccataccgag ggacggccgc agatcgtgta
     7681 ccgcccttcc agcaccgaag aggtggccag gattgtgcaa cgatgccatg acgcgggtca
     7741 gcgcatcacg atacagggcg gaatgacagg tgtggccggc ggcgcagtcc ccggcgacgg
     7801 cgatgtcgtg atcaacctgg agcgcatgaa ccgcatcgaa agcatcgacg cgctcgaagg
     7861 cgtgatgcag gtgcaggcag gcgcgacgct gcagcaggtg caggaggctg ccgccgagca
     7921 gggctggatg ttcgcggtcg acctcggtgc gcgaggaagt tgccagatcg gcggcaacgc
     7981 agccaccaac gccggcggcg tgcgcgtgat ccgctacggg acgatgcgcg attcggtact
     8041 cggcgtggag gcggttctgg ccgatggcag cgtggtctcg tcgctgacgc ggctcgtcaa
     8101 gaacagtacc ggcctcgacc ctcgcttcct gttcgtcgga acagaaggga ccttgggcat
     8161 catcacccgc ctgacgctgc gtctgcaccc gccgaccggc gaaccggcaa ccgcattggt
     8221 tgcggcggac aacttcgatg acctgccggt cctgctccgc gcgctcaggc gtgatgtcgg
     8281 cagcgcgctg tgtgcattcg agttcatgtc cggcgcgttc atggaccttg ccactcgcct
     8341 gactggacgc acgaatccgc tcacaggtcg cgcttcctgg tacgtcctgg tggaagcggc
     8401 gggcatgccc ggggagagca tggaagatcg cctgcaggct gcgctggccg atgctctcga
     8461 gcagggcacg atcacggact gcgcagtggc tgcgtcgctg acccaggccg cggcgttctg
     8521 ggggctgcgc gaatcgatac cggaaatcct ttctcacttg aaaccggtgg caccgctcga
     8581 catcggcatg ccgtggggac acacggcgga ctatctgcaa acggtcgagc aggaactgca
     8641 gcagcgcctt cccgatgcgc agcatctgtt cctcgggcac ctcggagaca acaacctgca
     8701 cctgatcagc ggcccggtcg atccggccgg ggtgcatgcg gtcgacgaaa tcgcatacgg
     8761 cgcgctgcgc gatcgcaacg gcaccgtcag tgcggagcac ggtgtcggcc gactgaagaa
     8821 ggagtacctg cacgtcaccc gcaacgcgag cgagatcgcg ctgatgaagc gtctgaaaca
     8881 ggcgctggac ccgaagcaga tcctgaaccc gggaaggatt ttcgactgaa tccgaaagaa
     8941 cggtaagccc gtggcgcaag tgcgccatgc attcgccttc aatcacaaca catgccggtt
     9001 tgcaacggcg caaggagaca gcaatgaacc gcagacattt tctcgcaacc cttggcgtgg
     9061 cttccatgag ttccacgtca ctgctcagta cagcatgggc cgccagctac ccgagttccc
     9121 cggtgcgcat cgtggtgccc tacgccgccg gcggcggagc tgatggcgtc acccgagtgg
     9181 tggcgcaact gctcggcgaa agactgaaag gctcgttcat cgtcgacaac cggcccggtg
     9241 ccgggggcgt gatcggtgcg cagaatgtcg cccgcgccga cgccgacggc tacaccctgc
     9301 tgatggccgg aaatccggaa ctcacgatca cgccgcacct gctcgccagg gcgccgtact
     9361 cggtggctga tgcgttcacg ccgatcatgc tggttgcgca atcgccgaat gtcttcgtcg
     9421 gcaatccggc cggtcccagg accctggccg cattgatcga agaaagccgc aagcgcaacg
     9481 cgccgatctc ggtcggcacg cccggtatcg gaa
//
LOCUS       DATLGF010000169         6545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_209703, whole genome shotgun sequence.
ACCESSION   DATLGF010000169 DATLGF010000000
VERSION     DATLGF010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6545
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209703"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VM406_05215"
     CDS             <1..288
                     /locus_tag="VM406_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75395.1"
                     /translation="VICRHSEPDSARNKETPVKAALAVLMLALSPLAFAHNCPNVMKE
                     IDAKLSSASGLSAETMERVRQLRAEGERLHKAGDHDESMRQLEQAKALLGA"
     gene            complement(323..808)
                     /locus_tag="VM406_05220"
     CDS             complement(323..808)
                     /locus_tag="VM406_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HVL75396.1"
                     /translation="MTPPSLPHSLSAFWQSFAASHGEAVDGRFYEAFHFSDNEQDARE
                     LAALVLAGTKRATASLLRSYEQSGQPLPYSGALSIVTNWAGEPVCVIETITVDVLPFD
                     SVPASFAAEEGEGDRSLGWWRDAHWRYFSRACRSHGYEPSATMPVVCERFRVVHVHGQ
                     R"
     gene            917..1690
                     /locus_tag="VM406_05225"
     CDS             917..1690
                     /locus_tag="VM406_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL75397.1"
                     /translation="MHLGLENKIAIVTGASQGIGKATAGALAEEGARVVLVARGQERL
                     DELAGQIRAAGGQAETVSADVSKADDCRRIIDEAVSRFGGIDILVNNAGSSATGAFEG
                     LDDEIWKADFDLKLFAAVRLAKLALPHMKQKRAGRIINVTNIGGKQPGAGTMPTTVTR
                     AAGLALTKALSKEYAPHNILVNTVCIGKVRAGQHESKAARAGVDIETFYERMAADIPL
                     GRVGEAEEAANAIVFLASEAASFITGTSINIDGGASAVL"
     gene            complement(1748..2608)
                     /locus_tag="VM406_05230"
     CDS             complement(1748..2608)
                     /locus_tag="VM406_05230"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HVL75398.1"
                     /translation="MSVRIPPRPALLLALQNEVAKETPHLKRLTDIIARDPALAGSFI
                     GIANSPFFYRRQEIRTVHDAVNMLGIDQLACVLAGLVARQIIGFGGKLLPRFWDVSEK
                     RAKGLSFVTRELDIAPPALAYNLGLFCDIGIPLMMSRFPNYRETLALANRFSDARFLH
                     VENERHKTNHTEVGALLSATWGISPEVTAAIRFHHVPLDTIGEAARTRHLIACNMLVE
                     KAIDEFRGERDRGHVSQDEHAAAESLGLSAADLDDLCDRLVLRFAGMAEWNSRGDTVR
                     PSARREYFAS"
     gene            complement(2765..3097)
                     /locus_tag="VM406_05235"
     CDS             complement(2765..3097)
                     /locus_tag="VM406_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HVL75399.1"
                     /translation="MKDWMFLGMAILAEVAATSALKSSAGFTRLGPSILVVIGYACAF
                     YFLSLTLRTIPVGVAYAIWAGLGIVLISLIGLFVFGQTLDAPALIGMGLIIAGVVVMY
                     AFSRSVPH"
     gene            3239..3688
                     /locus_tag="VM406_05240"
     CDS             3239..3688
                     /locus_tag="VM406_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75400.1"
                     /translation="MSDGWYQAAIRRLCDSAGIAEWEAVVAIRQIEAGGYPVALFPEP
                     DGERALGVYVELTNRAVTDAALLHRLLEANIGGAGGGFCLIPGADRIAFRSRVANADR
                     LSGPALMSCILDLAMRAGTSLREALHTDTAPVPRGRRAAPAGRETQA"
     gene            3685..4629
                     /locus_tag="VM406_05245"
     CDS             3685..4629
                     /locus_tag="VM406_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75401.1"
                     /translation="MNGIPFAPALPSVRAPGTLAAPRNAALRCLTGATHNVNDAVGRV
                     PCRRLFYSDAGLGANNAKSDRHFIRHQLSTDTSSTVRNLFKFDVEDAAAKREAYREAY
                     RKVHGRVPQNHEIMEQRPWEVLKFADRGVLDQAGTPYVRYYRQAVETAQARGGKVHFY
                     LGGFDAGEYVSSIVRTEARERKPFADYAANVDFNTLVGAGPHGNAGNANVYLWDSREG
                     AAGSTPLLERQPKITTIELYDATCGEFIKAGRGFHYYAENDVRLDTDCLLALQARLLE
                     QARRPENAALLLDGQEMAAPGRALLAQFQRYLDIGRTG"
     gene            complement(4646..6403)
                     /locus_tag="VM406_05250"
     CDS             complement(4646..6403)
                     /locus_tag="VM406_05250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HVL75402.1"
                     /translation="MRQTPNRVATALLFLLTWLISAAVSAANLEQVNGFGINPGNLLM
                     FQYVPRDLEPSRPLVVALHGCTQSAADYDDETGWVMLAERLRFALLLPQQQHANNERL
                     CFNWFEPRHNARGSGEAESVKEMIDKMEADHRVDSERIYVTGLSGGGGMTAVMLAAYP
                     DVFAGGGIIAGIPYRCAGNVTEALTRCGVFGGAMRDLTPRQWGDRVRDASTYPGPFPR
                     VSIWHGSDDATVKPKAMQELMEQWTDVLGIDQKPEISETLMGRIRHEVYEDLDGNAQV
                     ETWLVGGMGHGTPIDPGPGEEQCGVRPHAEHIIEAGICSSLRIARFWGLTNRAPTITI
                     TSAEASGNTLTVRGTASDEDGFVVKVAVSLDGVHPQAARDGHGTDAWSATFENLPPDA
                     RYVPVVSVRDDQGATTTIRAQQPVVIGEVAPNNPPSVRIDAVDVDRSCVLVHGRATDP
                     DPDSPVDRVEVSLGTRPFIAAVLDGAGYRAIECELEPGTYTTRARAFDAFGAASAIAA
                     GTPASVDRHANTTLGSWRDHMNADRIRVYQPPCRTGFGTCDELFVNLVMKYGEKPFPL
                     FRGLRSDAWFLDEAAALRN"
BASE COUNT         1180 a         2085 c         2165 g         1115 t
ORIGIN      
        1 gtgatatgtc gtcattccga acctgattct gcccggaaca aggagactcc cgtgaaagct
       61 gccctggccg tcctgatgct ggcgttgtcc ccgttggcgt ttgcgcacaa ctgtccgaat
      121 gtgatgaagg aaatcgatgc caagctgagc tcggcaagcg gcctcagcgc cgaaacgatg
      181 gagcgggtcc gccagttgcg cgccgaagga gaacgtttgc acaaggcggg cgatcacgac
      241 gaatcgatgc ggcagctgga acaggcgaag gccctgctcg gggcatagga cgggcatggt
      301 gcctgccgac gcgcgggcac cttcagcgtt ggccgtgcac atggacgacg cgaaaccgct
      361 cgcacacgac cggcatcgtt gcgctgggct cgtaaccgtg cgagcggcac gcgcgcgaga
      421 aatacctcca gtgggcgtcg cgccaccacc ctagtgatct gtcgccttcg ccttcttctg
      481 cggcgaatga agccggcacg ctgtcgaacg gcaggacgtc caccgtgatc gtttcaatca
      541 cgcataccgg ttcgccggcc cagttcgtga cgatgctgag cgctcccgaa tacggcagcg
      601 gctggccgct ctgctcgtag ctgcgcagca ggctggcggt ggcccgtttc gtcccggcca
      661 gcacgagcgc ggccagttcg cgcgcatcct gctcgttgtc ggaaaagtgg aaggcttcgt
      721 agaaccggcc gtccaccgcc tcgccgtggc tggccgcgaa ggattgccag aaggcggaca
      781 ggctgtgggg gaggctggga ggggtcatcc gcctgattct agatccagtg cgacacgtcg
      841 gcactgagaa gccttcggaa cgattgccgg cgcctcccta taatgactgt ccaactgtca
      901 agctttgggg aagcacatgc atctgggatt ggagaacaag attgcgatcg tgaccggcgc
      961 atcgcaagga ataggcaagg ccaccgcagg cgctctggcc gaagaaggtg cacgcgtggt
     1021 gctcgttgcg cgtggccagg agcgactcga cgagctcgct ggccagatcc gggcggcggg
     1081 cggacaggca gagacggtat cggcagacgt cagcaaggca gatgactgcc ggcgcatcat
     1141 tgacgaggcc gtctcccgct tcggcggcat cgacatcctg gtcaacaacg ccggttcatc
     1201 ggccaccggc gcgttcgagg gactcgacga cgagatctgg aaagcggatt tcgacctgaa
     1261 gctgttcgcc gcggtccggc tcgcgaagct ggccttgccg cacatgaagc aaaagcgcgc
     1321 gggccgcatc atcaacgtca ccaacatcgg cggcaagcag ccgggagccg gcacgatgcc
     1381 gaccacggtc acgcgcgcgg cggggctggc gctgacgaag gcgctgtcga aggaatacgc
     1441 accgcacaac atcctggtca acacggtgtg catcggcaag gtccgcgcgg gccagcatga
     1501 gagcaaggcc gcgcgtgccg gcgtcgatat cgaaaccttc tatgaaagga tggccgccga
     1561 tatcccgctc ggccgggtcg gcgaggccga ggaggcggcg aatgcgatcg tgttcctcgc
     1621 gtcggaggcg gccagcttca tcaccggcac cagcatcaat atcgacggcg gcgcttcggc
     1681 cgtgctgtag cgttgccgct tgcgccggag cgaccatcgc gatgcggagg ggggcggggc
     1741 gccggaatca ggaggcgaaa tattcgcggc gagcggacgg gcggaccgtg tcgccgcggc
     1801 tgttccattc tgccatcccg gcaaaacgca ggacgagccg atcgcataga tcgtccaggt
     1861 cggcggcgga gagtcccagg ctttctgctg ccgcgtgctc gtcttgcgac acgtgtcccc
     1921 gatcgcgctc gccgcggaat tcatcgatcg ccttttcgac aagcatgttg catgcgatca
     1981 ggtggcgggt ccgggctgcc tcgccgatcg tgtccagcgg cacgtggtgg aagcggatgg
     2041 ccgcggtcac ttcgggggag attccccagg tggcgctcag caaggcgccg acctcggtgt
     2101 ggttggtctt gtggcgttcg ttctcgacat gcaggaagcg cgcatcggaa aagcggttcg
     2161 ccagcgccag ggtctccctg tagtttggga agcgggacat catcagcgga atgccgatgt
     2221 cgcagaacag cccgaggttg taggcgaggg cgggcggcgc gatgtccaat tcccgggtga
     2281 cgaaactcag tcctttcgcc cgtttctcag acacatccca gaagcgcggc agcagctttc
     2341 cgccgaaacc gatgatctgc cgtgccacca gcccggcgag gacgcaggcc agctggtcga
     2401 tgccgagcat gttcaccgcg tcgtgcaccg ttcggatttc ctgccgcctg tagaaaaacg
     2461 gcgaattggc gatgccgata aaactgccgg ccagcgcagg atcgcgggca ataatgtccg
     2521 tcagccgctt caggtgcggc gtctcctttg ccacctcgtt ttgcagcgcc agcagcaagg
     2581 ctggacgtgg aggaatgcgg acggacatgc aggccctgca aggctacgta gagggtatcg
     2641 ggcctgcgac tgcgacgacc gcaggcgaca gataaccgcg gctccgatgg aaagtcagca
     2701 ctctagcgga aggcggcagt ggcgcacata ccataaacaa gggaggcgga accgggggcg
     2761 ccggtcagtg cggcaccgag cgcgagaacg cgtacatcac taccacgcct gcgatgatca
     2821 ggcccatccc gatcagggcc ggcgcgtcca gcgtctgccc gaagacgaac aggccgatca
     2881 ggctgatcag cacgatgccc agtccggccc agatcgcgta ggcgacgccg accgggatcg
     2941 tgcgcaaggt cagggacagg aagtagaacg cgcacgcata gccgatcacg accaaaatcg
     3001 acgggccgag tcgggtaaag ccggcgctgg acttgagcgc ggaagttgcg gcgacttcgg
     3061 caagaatggc cattccaaga aacatccagt ccttcatgct cgatctccgg tgcgaaatgg
     3121 gaatcggcga ttatcaccgg ccttcgccat tggcccgggc ttgtgccatg cgatgcccaa
     3181 tggaaccgtc ccggtactgt cgccactgat agcggacacg cttcggatgg agatcggcat
     3241 gagcgacggc tggtatcagg cagcaatacg cagattgtgc gacagcgcgg gaattgccga
     3301 atgggaggcg gtcgttgcca tcaggcagat cgaggccggc ggatatccgg tagcgctgtt
     3361 tcccgaaccg gacggggagc gtgcactggg ggtgtatgtc gagctgacga atcgtgcggt
     3421 gacggatgcc gcgctgctgc accgcttgct ggaggcgaac attggcggcg ccggcggcgg
     3481 attctgtctc atacccgggg ccgatcgcat cgcgtttcgc tcgagggtag ccaatgccga
     3541 ccgcctgtcc ggcccggcac tgatgtcatg catccttgat ctggcgatgc gtgccggcac
     3601 atccctgcgg gaggcgctgc ataccgacac cgcaccagtg ccgcgcgggc gccgcgctgc
     3661 tccggccggc agggaaacgc aggcatgaac ggcatcccgt tcgcgccggc cctgccttcg
     3721 gtgcgcgcac ccgggacgct ggcggcgccg cgcaatgccg cgctgcgctg cctgaccggg
     3781 gcaacgcaca atgtgaacga tgccgtcggc agggtgccgt gccggcgcct gttctattcg
     3841 gacgcaggcc tgggcgcgaa taacgcgaag tccgaccggc atttcattcg ccaccagctc
     3901 agcaccgaca ccagttccac cgtccgcaat ctgttcaagt tcgatgtcga ggacgctgca
     3961 gcgaaacggg aggcgtaccg cgaggcgtac cggaaggttc acggccgcgt gccgcagaac
     4021 cacgaaatca tggagcaacg cccgtgggaa gtgctgaagt tcgccgatcg cggcgtgctt
     4081 gaccaggcgg gcacgcccta tgtgcgctac taccggcagg cggtcgagac agcgcaggcg
     4141 cgcggcggaa aggtgcattt ttatctgggc ggtttcgacg ccggcgagta tgtgtcgagc
     4201 atcgtcagga ccgaagcgcg cgagcgcaaa cccttcgccg actatgcggc gaacgtggat
     4261 ttcaacacgc tggtcggcgc gggcccgcac ggcaacgccg gcaatgccaa tgtctacctg
     4321 tgggattccc gcgaaggcgc ggccggcagc acccccttgc tggagcggca gccgaagatc
     4381 accaccatcg aactctatga cgcgacttgc ggggagttca taaaagccgg acggggattc
     4441 cactactatg cggagaacga tgtgcggctc gacaccgact gcctgctcgc actgcaggcg
     4501 cgcttgctcg agcaagcgcg caggcccgag aatgccgcgc tgctcctcga cggccaggag
     4561 atggcagcac ccgggcgggc gctgctcgca cagttccagc gctacctcga tatagggcgc
     4621 accggctgaa agcaccgggc gccgttcagt tccgcagcgc agccgcttcg tccaggaacc
     4681 aggcgtccga ccgcaggccc ctgaacagcg gaaagggctt ttccccatac ttcatgacca
     4741 ggttcacgaa gagctcgtcg caggtgccga atcctgtgcg gcacggcggc tggtagacgc
     4801 ggatgcgatc cgcattcatg tgatcgcgcc atgacccgag tgtggtgttg gcatgcctat
     4861 cgacgcttgc aggcgtgccg gcggcgatgg cgctagccgc gccgaacgcg tcaaatgcgc
     4921 gggccctcgt cgtgtacgtt cccggttcga gttcgcactc gatcgcgcga tagccggcgc
     4981 cgtcgaggac ggccgcgatg aacggccttg tccccagcga tacctcgacc cggtctaccg
     5041 ggctgtcagg gtccgggtcc gttgcccggc cgtgtacgag cacgcagctg cgatccacgt
     5101 ccaccgcgtc gatgcgcacg gaaggcgggt tgttcggcgc cacctcgccg atgactaccg
     5161 gctgctgcgc ccgtatggtc gtggtcgcgc cctgatcgtc gcgcaccgag accacgggta
     5221 catagcgtgc atcgggcggc aggttttcga aggtggccga ccaggcatcg gtgccgtgtc
     5281 catcgcgcgc ggcctgggga tggacgccgt ccagactgac cgcgaccttg accacgaagc
     5341 catcctcgtc cgacgccgtg ccgcgcacgg tgagcgtgtt gccgctggct tcggccgacg
     5401 tgatcgtgat cgtcggggcg cgattggtga gtccccagaa ccgcgcgatc cgcaggctgg
     5461 aacagatgcc cgcttcgatg atgtgttcgg cgtgcggcct caccccgcac tgctcttcgc
     5521 caggtccggg atcgatcggc gtgccatggc ccattcctcc gaccagccag gtttccacct
     5581 gcgcattgcc gtccaaatct tcgtacacct cgtgtcgtat ccgtcccatc agtgtttcgc
     5641 tgatttccgg cttctggtcg atgccgagca cgtcggtcca ctgctccatc agttcctgca
     5701 tcgctttcgg cttcaccgtc gcatcatcgg agccgtgcca gatcgacacg cgcggaaagg
     5761 gccccggata ggtggaagcg tcccgtaccc ggtctcccca ctggcgcggc gtgagatcgc
     5821 gcatggcgcc gccgaacaca ccgcagcgcg tgagcgcctc ggtgacattg ccggcgcagc
     5881 ggtacggaat gccggcaatg atgccgcctc cggcgaacac atccggatag gccgccagca
     5941 tcaccgccgt catgccgccc ccaccggaca ggccggtgac atagatgcgt tcactatcga
     6001 cgcgatggtc ggcttccatc ttgtcgatca tttccttgac cgattccgct tccccgctgc
     6061 cgcgcgcgtt gtgccgcggc tcgaaccagt tgaagcacag gcgctcgttg ttcgcatgct
     6121 gctgctgggg cagcagcaat gcgaaacgca gccgctcggc gagcatgacc cagccggtct
     6181 cgtcgtcgta gtccgccgcg ctctgcgtgc atccgtgcag cgccaccacc agcggcctgg
     6241 atggttccag gtcgcgcgga acatattgga acatcagcag attgcccgga ttgatcccga
     6301 agccgttaac ttgctcgaga ttggctgccg atacggccgc actgatcagc catgtcagca
     6361 ggaacaggag tgccgttgcg acacgattcg gagtttgcct catgatgacc tccttctgcc
     6421 caatgtcctg ctcgccatgt gttcgcgagc ctcgcacgtg cggccttcgg ctccttgtgc
     6481 tcgctcaagc cgcaccggat ttgcgcatct agtttgatgg cagggcccat cagcgcagcg
     6541 aggac
//
LOCUS       DATLGF010000170         3908 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_23305,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000170 DATLGF010000000
VERSION     DATLGF010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23305"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..2054
                     /gene="ggt"
                     /locus_tag="VM406_05255"
     CDS             270..2054
                     /gene="ggt"
                     /locus_tag="VM406_05255"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HVL75403.1"
                     /translation="MSRRTSLLSSLAIPVLLAACAGAPVQQEPGMQAPERATGLTEQR
                     GATAQNFMVAAANPLATQAGQRILQAGGSALDAAIAVQMVLTLVEPQSSGIGGGAFLL
                     HTDGKRVQAYDGRETAPARVDENLFRGPDGKALAFHDAVVGGRSVGVPGVLRMLERAH
                     REHGRLQWAALFAPAIALADEGFPVSQRLHTMLKSEQHLKKDPTAAAYFYDGAGEPHP
                     VGYRLRNPELAQVLREIASGGADAFYTGRLAKEIEAKVQGHPGNPGALSAADIAGYQA
                     REREPICTDYRRWTVCGMPPPSSGGIAVAQMLGMLETRDMAALRPVDGVPSAEAIHLF
                     SEAGRLAYADRGRYVADSDFVPLPGNSPAALLDARYLQMRARLIGERSMGKAEPGTPP
                     ATALALGTDRSPELPSTSHVSIVDAYGNALSMTTTIEDAFGSRQMVRGFLLNNQLTDF
                     SFEAADAQGPIANRVEPGKRPRSSMAPTLVFERGTRELVLSAGSPGGSAIINYVAKVL
                     VGTLDWELNVQDAISLPNFGSRNGPTEVEQGRVPEAVIESLRARGHNVRVMEQTSGLQ
                     GIMRISREGRAAWFGGADPRREGAVMGD"
     gene            2120..3340
                     /locus_tag="VM406_05260"
     CDS             2120..3340
                     /locus_tag="VM406_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVL75404.1"
                     /translation="MGNDKKTALVLTGGGARAAYQIGVLRGVADVLAASGGQAGRNPY
                     PIICGTSAGAINAAALACHADDFGAGLGFVESVWEDITAEQVYRSDSLGVLRSGARWL
                     SLLSFGWLLRTWKSNPPNSLLDNTPLVTLLNRMLDFPRLDAALANGALHALAVTASSY
                     TAGRHITFYQSEAAITPWTRSQRVALPAQIGVEHLLASSAIPLIFPATPIYCDGAMEY
                     CGDGSMRQLAPISPAIHLGADKVLVVGAGRLNDHETSPTGNVGYPSLAQIGGHAMSSI
                     FLDSLAVDIERMNRINHTLSLLSEEQRKATPLRPIEMLIIAPSERLDRMAVRHVESLP
                     GPIRMLLGAIGATDAGGAALASYLLFEPGYTRELMALGRADALAREKEILLFFGEADT
                     PAQRPTAALETGWP"
     gene            complement(3347..3730)
                     /locus_tag="VM406_05265"
     CDS             complement(3347..3730)
                     /locus_tag="VM406_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75405.1"
                     /translation="MIRHSSRLVFSLAAVALIAAVLPSVASARSDVTWSVSIGSSHPA
                     PVYVAPPPVVYVQPQPVYVQPRPVYVQPQPVYVQPQPVYVQPRPVYVQPGTVIHVGTP
                     VYVHEPRHRKGKHHHHRKHPHEYRY"
BASE COUNT          671 a         1256 c         1306 g          675 t
ORIGIN      
        1 agatgggtgc ggacgcggtc gacatcggca agaccatcca cccgcatccg acgctcggcg
       61 agtccatcgg catggcggcc gaggtcttcg agggcgtctg cacggacctg ccgccggtta
      121 agaagaaata gcgatggaac agggcggcgc tgccgccctg ccttcctgct gagcaaacac
      181 aaatcctgtg aagaaagggc ggcgtgtcta gtgacacgtg catgctgccg gttcaagctg
      241 gtgtttcgtc cacgcatcag gagcccgtca tgtccaggcg cacgagccta ctctcttccc
      301 tcgccatccc cgtcctgctg gcggcctgcg ccggcgcacc ggtgcagcag gaacccggca
      361 tgcaggcgcc tgaacgcgcg accggcctta ccgaacaacg cggcgccact gcgcagaatt
      421 tcatggtcgc cgccgcgaat ccgcttgcga cccaggccgg gcagcgcatc ctgcaggcag
      481 gcggttcggc gctcgatgcc gcgatagcgg tccagatggt gctgacactg gtcgagccgc
      541 aatcgtcggg catcggcggc ggcgcattcc tgctccatac cgacggcaaa cgggtccagg
      601 cctacgacgg gcgcgagacc gcgccggcgc gcgtggacga aaacctgttc cgcggaccgg
      661 acggcaaggc gctggccttt catgacgcgg tggtgggcgg acgctcggtc ggcgtgcccg
      721 gcgtgctgcg catgctcgag cgtgcgcacc gcgagcacgg ccggctgcag tgggcggcgc
      781 tgttcgcccc ggcgatcgcg ctcgccgacg agggcttccc ggtcagccag cgcctacata
      841 ccatgctcaa gagcgagcaa cacttgaaga aggacccgac ggcggccgcc tatttctatg
      901 acggggccgg tgaaccgcat ccagtcggct atcgtctgcg caatccggaa ctggcgcagg
      961 tgctgcgcga gatcgcatcc ggtggcgcag acgcgttcta cactggccgc ctcgccaagg
     1021 agatcgaggc caaggtgcaa gggcatccgg gcaatcccgg cgcgctttcc gccgcggaca
     1081 tcgccggcta ccaggcgcgc gagcgggagc cgatctgcac cgactaccgg cgctggacgg
     1141 tttgcggcat gccgccgcct tcgtccggcg gaatcgcggt cgcgcagatg ctgggcatgc
     1201 tggaaacgcg cgacatggcc gcgttgcgcc cggtcgacgg cgtgccgagc gcggaggcga
     1261 tccatctgtt ctccgaggcc gggcgcctcg cctatgccga ccgcgggcgc tacgtagccg
     1321 acagcgattt cgtcccgctg cccggcaaca gtccggcggc gctgctcgac gcgcgctacc
     1381 tgcagatgcg cgccaggctg atcggcgagc gctcgatggg caaggccgag ccgggcacgc
     1441 cgcccgccac cgcgctggcc ctgggcaccg accgctcacc cgagctgccg tccacctcgc
     1501 atgtgtccat cgtcgacgcc tacggcaatg ccttgtcgat gacgaccacg atcgaagacg
     1561 cgttcggctc gcgccagatg gtgcgcggct tcctgctcaa caaccagctg accgacttct
     1621 cattcgaagc agcggacgcg caggggccga tcgccaaccg cgtcgaaccc ggcaagcgtc
     1681 cgcgcagctc gatggcgccg acgctggtct tcgagcgcgg cacgcgggag ctggtgctgt
     1741 cggccggttc gccaggcggc tcggccatca tcaattacgt cgccaaggtg ctggtcggca
     1801 cgctcgattg ggaactgaac gtgcaggatg cgatcagcct gccgaacttc ggcagccgta
     1861 acggccccac ggaggtggag cagggcaggg tgccggaggc ggtcatcgag tcgctcaggg
     1921 cgcgcggcca caatgtgcgc gtaatggaac agacctccgg actgcaaggc atcatgcgca
     1981 tctcacggga gggacgagcc gcatggttcg gcggcgccga cccgcgtcgc gaaggcgctg
     2041 tcatggggga ctgaccgacc ggagcgaagc acgcgacttt tcttgtctga tggaagggag
     2101 gtcgctatag tcgtttccca tggggaacga caagaaaacg gcacttgttc tgaccggagg
     2161 tggtgcgcgt gccgcgtacc agatcggcgt gctgcgggga gtagccgacg tgctggccgc
     2221 aagcggcggg caggccgggc gcaaccccta tccgatcatc tgcggcacct ccgcgggagc
     2281 gatcaatgcc gccgccttgg cctgtcacgc ggatgatttc ggcgctgggc tcggcttcgt
     2341 agagtcggtc tgggaagaca tcactgccga gcaagtgtat cgctcggact cgctcggcgt
     2401 tctgcgctcc ggcgcgcgct ggctgtcgct gctttcattc ggctggctgt tgcgtacctg
     2461 gaagtcgaac ccgcccaatt cactgctgga caacacacct ctggtcacgc tgctcaaccg
     2521 catgctggac tttccgcgcc tcgatgcagc cctggcgaac ggtgcattgc acgcgctggc
     2581 cgtcaccgcg tcctcgtata cggccgggcg gcacatcacc ttctaccaga gtgaagccgc
     2641 gatcacgccc tggacgcgct cgcagcgcgt cgcgctgccg gctcagatcg gcgtcgaaca
     2701 tctgcttgct tcgtcagcca ttcccctgat ttttccggcc acgccgattt attgcgatgg
     2761 tgcgatggag tactgcggcg atggctcgat gcgtcagctt gcgccgattt caccggcgat
     2821 ccatctcggg gccgacaagg tgctggtggt gggggcagga cggctgaacg accatgaaac
     2881 ttccccgacg ggcaatgtcg gctacccgag cctggcccag atcggcggac atgcgatgtc
     2941 aagcatcttt ctcgacagcc tcgctgtgga tatcgagcgg atgaatcgca tcaaccacac
     3001 gctgtcgctc ttgagcgagg aacagcggaa agcaacaccc ctgcggccga tcgaaatgtt
     3061 gatcatcgcg ccttcagagc gtctggaccg gatggcggtc cgacatgtgg aatcgctgcc
     3121 ggggccgatc aggatgctgc ttggtgcgat cggcgcgacc gacgcaggcg gtgctgcgct
     3181 ggcgtcttat cttttgttcg aaccgggcta tacccgggaa ctgatggccc tgggcagggc
     3241 cgacgcgctg gcgcgcgaaa aggagattct gcttttcttc ggtgaagcgg atacgccggc
     3301 ccagcgcccg acagcggcgc tcgagaccgg ctggccctga atcgaatcag tagcgatatt
     3361 cgtgcgggtg cttacggtgg tggtggtgct tgcccttgcg gtggcggggt tcgtgcacgt
     3421 agaccggcgt gccgacatgg atgacggtgc cgggctgcac ataaaccggg cgcggttgga
     3481 catagaccgg ctgcggctgc acatacaccg gttgcggttg tacgtacacc gggcgcggct
     3541 gtacataaac cggctgcggc tgcacgtata caacaggcgg cggcgccaca tacaccggag
     3601 cggggtggct gctgccgatg ctgaccgacc aggtgacgtc tgaacgtgca ctggcaaccg
     3661 agggcaggac tgcggcaatc agggcaacgg cggcaaggct gaagacaagg cgggaagaat
     3721 gacggatcat ggggtttctc ctgaggattc cggcttcatt aaacgtcatg ccctgttcct
     3781 ccagcatgtt tgacaagtaa gacctgcaac aatttgtaat ccgacaagcg gttgtgctgg
     3841 cccccaagca aggttaatct tgcttcgcct cgtcttattg ccacactttc aatgctcttc
     3901 ctacgtcg
//
LOCUS       DATLGF010000171         5251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_38969,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000171 DATLGF010000000
VERSION     DATLGF010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38969"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /gene="motA"
                     /locus_tag="VM406_05270"
     CDS             complement(<1..840)
                     /gene="motA"
                     /locus_tag="VM406_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HVL75406.1"
                     /translation="MLVIVGYLIVCGAVFGGYALAGGHLAALFQPLELLMIGGGAVGA
                     FFVGNNAKAIKATLKALPTLFKGSRYNKALYMELMTLLFELLSKVRKEGLMSIEGDID
                     KPEESPIFSKYPGVMADHHIIEFMTDYLRLMVSGNMDAFQIENLMDNEIETHHAEAEI
                     PVHTIAKVGDALPAFGIVAAVMGVVHTMGSVGLPPAELGLLIAHALVGTFLGILLAYG
                     FVSPLAALLEQKLHESSKMYQCVKVTLLASLNGYAPALAVEFGRKVLFSTERPSFSEL
                     EDHI"
     gene            complement(1045..1803)
                     /locus_tag="VM406_05275"
     CDS             complement(1045..1803)
                     /locus_tag="VM406_05275"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL75407.1"
                     /translation="MSSILRLPALRAFVVQIAALAVVLCLAAVAREAFDVDLGLAAGS
                     VLQGVTAAAIAWRSRAEPWWLAIHLVFVPLLMVVLAADIPSWVFLLAFCAMLALYGQI
                     FRTRVPYFPSTPAVRAAVGAVLPPGPVRFIDIGSGFGGLPIRLARERPDGRFEGIELA
                     LFPFLGSYLRACLTGSRARLRHGDYTRLDLHAYDVVFAYLSPAAMPGLWAKATAEMAP
                     GSLLLSFEFGIPGSPPDLVLQPAPGQPPLYGWRF"
     gene            complement(1847..2401)
                     /gene="flhC"
                     /locus_tag="VM406_05280"
     CDS             complement(1847..2401)
                     /gene="flhC"
                     /locus_tag="VM406_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HVL75408.1"
                     /translation="MTKKSVVTEAQEIQLAIELIQLGARLQLLESETSLSRERLLKLY
                     KELRGVSPPKGMLPFSTDWFITWQPNIHSSLFINIHKYLIEHAQVSGIEAVMKAYKLY
                     LEQVGMGPDGEPVLSLTRAWTLVRFFESKMLETACCSKCGGDFVVHRLDLHADYVCGL
                     CHMPSRAGKTKKAREDGAAAAAQV"
     gene            complement(2442..2759)
                     /gene="flhD"
                     /locus_tag="VM406_05285"
     CDS             complement(2442..2759)
                     /gene="flhD"
                     /locus_tag="VM406_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HVL75409.1"
                     /translation="MNTNDMLAEIRDANLGYLMLAQQMIRSDKASAVFRLGLSSEIAD
                     LIEGLNNAQILKLASTNMMLARFRFDDGAILGMLTNYNKDRELAHSHAAILMAGQPLE
                     EIS"
     gene            complement(3523..3616)
                     /locus_tag="VM406_05290"
     tRNA            complement(3523..3616)
                     /locus_tag="VM406_05290"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3580..3582),aa:Ser,seq:gct)
     gene            complement(3714..4964)
                     /locus_tag="VM406_05295"
     CDS             complement(3714..4964)
                     /locus_tag="VM406_05295"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010396891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HVL75410.1"
                     /translation="MALIVHKYGGTSMGSTERIKNVAKRVAKWHDAGHQIVVVPSAMS
                     GETNRLIALAKEVMPQPDPRELDMLASTGEQVSVALLSMALQSLGKSAVSYAGWQVPI
                     KTDSAFTKARIQSIDDAKVRRDLDAGRIVIITGFQGVDEAGNITTLGRGGSDTSAVAV
                     AAAMKAQECLIYTDVDGVYTTDPRVVSEARRLRTITFEEMLEMASLGSKVLQIRSVEF
                     AGNYKVPTRVLSSLTDPLMPLEEEANSGTLISFEEDTHMEQAVISGIAFNRDEAKITV
                     LGVPDKPGIAYQILGPVADANVEVDMIIQNQSVDGKTDFTFTVARGEYARAMDVLNKS
                     VKEHIQAGSIVGDAKVSKVSVVGVGMRSHVGIASQMFRTLSEEGINIQMISTSEIKIS
                     VLIDEKYMELAVRALHRAFELDKG"
     gene            complement(5053..>5251)
                     /locus_tag="VM406_05300"
     CDS             complement(5053..>5251)
                     /locus_tag="VM406_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TilS substrate C-terminal domain-containing
                     protein"
                     /protein_id="HVL75411.1"
                     /translation="HHFQARAIPPWERRRLPVLVAGARLLFAAGIGMNVRDIPWSHDA
                     GVSISWRPETGAFPRAAGVPV"
BASE COUNT         1074 a         1654 c         1603 g          920 t
ORIGIN      
        1 gatgtggtct tccagctcgg agaacgacgg gcgttccgtg gaaaacagca ccttgcgtcc
       61 gaactccacc gccagcgccg gcgcgtagcc gttcaggctc gccagcagcg tcactttcac
      121 gcactggtac atcttcgacg actcatgcag cttctgctca agcagggcgg cgagcgggct
      181 gacgaagccg taggcgagca gaatcccgag gaaggtcccg accagcgcgt gcgcgatcag
      241 cagtccgagc tctgccggcg gcaggccgac cgagcccatc gtatgcacga cccccatcac
      301 cgccgcgacg atgccgaacg ccggcagcgc atcgccaacc ttggcgatcg tatgcacggg
      361 aatctcggct tctgcatgat gggtttcgat ctcgttgtcc atcaggttct cgatctggaa
      421 cgcgtccatg ttcccggaca ccatcaggcg caggtaatcg gtcatgaact cgatgatgtg
      481 gtgatcggcc atcacgcccg gatacttgct gaagatcgga ctctcctcgg gcttatcgat
      541 gtcaccctcg atcgacatca acccctcctt gcgcaccttg gagagcagct cgaacagcag
      601 ggtcatcagc tccatgtaga gcgccttgtt gtagcgcgag cccttgaaca gcgtcggaag
      661 cgccttcaga gtcgccttga tcgccttggc gttattgccg acgaagaagg cgccgacggc
      721 gccgccgccg atcatcagca gctcaagcgg ctggaacagc gcggcgagat ggccgcctgc
      781 gagggcataa ccgccaaaca cggctccgca gacgatgaga taaccaacaa tgactagcaa
      841 gtgcgctccg gagctgaaat gtgaagtttc ctgtggtcaa gcattgtgcc aattcatgca
      901 tggatcgaca accgcgcaga tcgaaaagca acgcccgagc gcactctggt ccgagcgcgg
      961 cgatgaaggc gcacttccat ccggttacgg cccatccacg gaaaacttga ccctgggtgc
     1021 gccgcattca tcgcagccgc cgcctcagaa acgccagccg tacagcggag gctggccggg
     1081 ggccggttgc agcaccagat ccggcggact cccgggaatc ccgaattcga agctcagcag
     1141 cagcgatcct ggcgccatct cggcagtcgc cttggcccac agccccggca tggccgccgg
     1201 cgacaggtac gcgaacacga cgtcataggc gtgcaagtca agccgtgtgt aatcaccgtg
     1261 gcgcaagcgg gcgcggctgc cggtcaggca cgcgcgcaga tagctgccca ggaatggaaa
     1321 aagcgccagc tcaatcccct cgaagcggcc atcgggtcgc tccctggcga gtcgtattgg
     1381 aagaccgccg aatccgctgc cgatgtcgat gaagcgcacg ggacccggcg gcagcactgc
     1441 gcccaccgct gcacgcacgg ccggcgtaga aggaaagtaa gggacacgtg tacggaaaat
     1501 ctggccgtac agcgcgagca tcgcgcaaaa ggccaggaga aacacccagg acggtatgtc
     1561 tgcggccagg acgaccatga gaagcggaac gaacaccagg tgaatcgcca gccaccacgg
     1621 ttcggcacgt gaccgccatg cgatcgcagc cgccgtcacc ccctgcagca cgctgccggc
     1681 cgccagcccg aggtcgacgt cgaacgcttc gcgcgcgacc gctgcaaggc aaaggacaac
     1741 cgcaagcgcc gcgatctgga caacgaaagc gcgcagcgcg ggcaatcgca ggatggatga
     1801 catcggaacg agatccggca gcccggccgg atacgaaagg caaggatcag acttgagccg
     1861 cggcagccgc cccgtcttca cgcgccttct tggtcttgcc ggcacgcgag ggcatgtggc
     1921 acaggccgca gacatagtct gcatgcaggt ccagccgatg cacgacgaaa tcgccgccgc
     1981 acttgctgca gcaggctgtt tcgagcatct tgctttcgaa gaagcggacc agggtccagg
     2041 cgcgcgtaag cgacagcacc ggttcgccgt ccggccccat gccgacctgt tccaggtaaa
     2101 gcttgtaggc cttcatcacg gcctcgatgc cggatacctg cgcgtgctcg atcaggtatt
     2161 tatggatgtt gatgaacagc gaggaatgga tattcggctg ccaggtgatg aaccagtcgg
     2221 tcgagaacgg caacatgccc ttcggaggcg acacgccgcg cagctccttg tagagcttga
     2281 gcaggcgttc acgcgacagc gaggtttccg attccagcaa ctgcaaacgt gcacccagct
     2341 gaatcagctc gatagcaagc tgtatctcct gcgcttccgt caccacgctt ttctttgtca
     2401 tgtcgacctc gtttcgtttc ccgtccggcg cggaagtgga atcaggaaat ctcttccagc
     2461 ggctggcccg ccatcaggat cgcggcatgg gaatgcgcaa gctcgcggtc cttgttgtag
     2521 ttcgtcagca tgccgaggat cgcgccgtcg tcgaaacgga agcgcgcgag catcatgttg
     2581 gtgctggcca gtttcaggat ctgcgcgttg ttcagaccct cgataagatc ggcaatttcc
     2641 gagctgagcc ccaggcggaa gacggcgctg gccttgtccg aacgaatcat ctgttgcgca
     2701 agcatcaggt atccgagatt cgcgtcacga atctccgcca gcatgtcatt cgtgttcatt
     2761 ttcgtctccc tgctacgttg gacacggcgc cccgcgccga ctcagtgagg ccgattctgc
     2821 tgaggaaatt actgtgtgga aataggaatg cgcccatatt tttcgtcggg cgaaaacgac
     2881 atgcacctgt aggagtctgt cctacaagca cagattcagg cagtggcaca gttccttctg
     2941 caacccgcaa acccgctctg gctgcgggtt tccagcggat cacgccaatc tgggccacca
     3001 gttgttggaa gaaataaacg taacaaatcg atacgcggaa gaaccctcgt gcccttcaaa
     3061 aaagcggcat tcagccctcg aaaacagggg cgcagccgcg cgcatgtgtt gtatatcggc
     3121 agcaacccag cccgacttta gggtttgcgt cgaaaaattt tcgcgccctc ggcagcacgc
     3181 gacgatggca tcgtttgcgc tgacaggaat ccggtggcaa tgctcgcccc gcaacaggcg
     3241 gcgaagccgc gcacggccga gacagcgcct gtcagccccg catcgagaaa ggcgccgacc
     3301 catgcctggc gtttacggcg ccgggcatgt ggattttagg gtgggatgca cttttgcctc
     3361 gaccaccgac gccgacgcgg ggcggctact tacccgccgc gcggcagggc gcggaggcag
     3421 cgagcaggag gcagggacgg aatcgacagg cgcagtatgg cccgggggca cggcacgcgc
     3481 atacaggcaa aaaaatgccc cttgcggggc atttttcaat tctggcggag acggagggat
     3541 tcgaaccctc gatacaggtt taagcccgta tgcttcctta gcagggaagt gccttcgacc
     3601 tctcggccac gtctccacgc gtcaagcggg accacgtccc gcttgagtaa agtcccgcat
     3661 gataaccgct cgcaccaact tggtcaatga aagccccatg cgggcgcgca gcatcaaccc
     3721 ttgtcgagct cgaacgcgcg gtgcagcgcc cgcaccgcca gctccatgta cttctcgtcg
     3781 atcagcaccg agatcttgat ttcggaggtc gagatcatct ggatgttgat gccctcttcc
     3841 gacagcgtgc ggaacatctg cgaggcgatg ccgacgtggc tgcgcatgcc cacaccgacc
     3901 acggagacct tcgacacctt cgcgtcacca acgatgctgc cggcctggat atgctccttg
     3961 acgctcttgt tgagcacgtc catcgccctc gcgtactcgc cgcgcgcaac cgtgaaggtg
     4021 aaatccgtct tgccgtcgac cgactggttc tggatgatca tgtcgacttc gacgttggcg
     4081 tcggcgaccg gaccgaggat ctggtaggcg atgcccggct tgtccggcac accgagcacg
     4141 gtgatcttgg cttcgtcgcg attgaacgcg ataccggaaa tgacggcttg ttccatgtgt
     4201 gtgtcttcct caaacgaaat cagggtgccg gaattcgctt cttcttccag cggcatgagc
     4261 ggatcggtca gcgacgaaag cacgcgcgtg ggcaccttgt agttgccggc gaactcgacc
     4321 gagcggatct gcaggacctt ggagcccagc gacgccatct cgagcatttc ctcgaaggtg
     4381 atggtgcgca ggcggcgcgc ttcggagacc acgcgcggat cggtcgtgta gacgccgtcg
     4441 acgtccgtgt agatcaggca ctcctgcgcc ttcatcgcgg ccgccaccgc aacggccgag
     4501 gtgtccgagc cgccgcggcc cagcgtggtg atgttgcccg cttcgtccac accctggaaa
     4561 ccggtgatga tcacgatcct gccggcatcg agatcgcgcc gcaccttggc gtcgtcgatc
     4621 gactggatgc gcgccttcgt gaacgcggaa tcggtcttga tcggcacctg ccagccggca
     4681 tacgagacgg ccgacttgcc cagcgactgc agcgccatgg acagcaacgc gaccgacacc
     4741 tgctcgccgg tcgaggccag catgtccagt tcgcgcggat ccggttgcgg catgacctcc
     4801 ttcgcgaggg cgatcaggcg gttggtttcg ccggacatgg ccgacggaac gacgacgatc
     4861 tggtggcccg cgtcatgcca cttggcgacg cgcttggcga cattcttgat gcgctcggtc
     4921 gagcccatcg aagtgccgcc gtatttatgg acgatcaaag ccatagggaa tagtgaggcg
     4981 aggaagggag aatctgaaga aaaatcaacc gcttaattta cctgctgcga tgcggaaaag
     5041 caagtcggca gcctataccg gaacgcccgc agcgcgagga aaggcgcccg tttcaggtcg
     5101 ccagctgatg gagacgcccg catcatgcga ccaggggatg tcgcgcacgt tcattccgat
     5161 gcccgcagcg aaaagcagcc gggcgccggc caccagcacc ggcaggcgcc gccgctccca
     5221 ggggggaatc gcgcgcgcct ggaagtgatg c
//
LOCUS       DATLGF010000172         4768 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_209707, whole genome shotgun sequence.
ACCESSION   DATLGF010000172 DATLGF010000000
VERSION     DATLGF010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209707"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1791)
                     /locus_tag="VM406_05305"
     CDS             complement(<1..1791)
                     /locus_tag="VM406_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVL75412.1"
                     /translation="MAKASESSQGDDPRKDGEDRKDEARRSESAGAVVEHGGGGKRVA
                     SRRGQYLVAPRRASELQVMGLQPLQFSMLEDTLRNSPDVEVIDTVGARNIVGALADGL
                     GDGPGGVLVVRMEDDKAATLQQQAQGRLIVEHDQSLSLLDTTSSLPNMVTGAVAHAGP
                     ALSVNVTVLGRDDKPVPDAEVYLFGSLLPATATTDQRGQAQLTLYGESESSVRGLYVK
                     PKADYWSFYQAQPALTTSEPNVVGLRPISDWPSLAAFPGQQTYGWGQKAMRLDQLPAE
                     YRGQGIRVAVIDSGTDNRHRDLGKIRFGFDIINKRTNPDTWNEDLIAHGSHCAGTIAG
                     ADNGRGIRGFAPDAEIHACKLFPGGQVSQLIDALEYCIEKQIDIVNLSLGGAQPSEAL
                     EQQIIRARRAGVACIAAAGNSGNRVQYPASSPNVLAVAAIGRLNEFPPDSYHMQTLTQ
                     PVDRSGVYAARFSCFGPEVAVCAPGVAVTSSVPPDNYAVWDGTSMAAPHIAGLAAFIL
                     AHHPDFQGAFGARGPERVERLFQIIRYSARALANVDPARVGYGLPDVLVALGLQPHPS
                     TQMIPQAAMAPAFFGGPAFGGGWPAVSPGAP"
     gene            complement(1809..2087)
                     /locus_tag="VM406_05310"
     CDS             complement(1809..2087)
                     /locus_tag="VM406_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75413.1"
                     /translation="MAADSVKDGRARYIVHAQDARRLADFVHEIRARDDVELVDLIGP
                     ADAPHIAVLHMPRATARELQERFRTTHQIFIEPDQPLSPLCGGVAGPL"
     gene            complement(2096..3010)
                     /locus_tag="VM406_05315"
     CDS             complement(2096..3010)
                     /locus_tag="VM406_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020487442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75414.1"
                     /translation="MYDYYQQGIPGGFGPVLQFAPQGLLGGMLGGPLGGMIGRGIGGM
                     FGNPSLGAQIGRAAGGIGGTLLPFGADPMSVYAQQPQFAPQGFFGNLIGQLGQPVGGA
                     IGGAFGNAGMGSAIGGAVGQLGRMLPFGIDPMSMAMLQQAALEQQAMQQQAMQQQLAP
                     QGFFGNLIGQLGQPVGSAIGGAFGNAGLGGAIGGAAGQLGRLLPFNVDPMSAAYLQQQ
                     FAPQGFFGNLIGRAAQPIGTTIGGAFGNPSLGGAIGNVAGQLGRMLPFGADPYAYWQQ
                     AAVYPGYAGFQGGAPALGGQQQGQPTIH"
     gene            3629..>4768
                     /locus_tag="VM406_05320"
     CDS             3629..>4768
                     /locus_tag="VM406_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase family protein"
                     /protein_id="HVL75415.1"
                     /translation="MTTRDVLFVGAARTAIGTFGGSLKDFSACDLGTIAVKEAFNRAG
                     VDPAAAGQIVLGNVIHCEPRDMYVSRVVGINAGMAKESAALTLNRLCGSGLQAIVTAA
                     NAIQLGDTDIAVGGGAETMSRAPYSTQAARFGQRMGDFKMIDMMVGALTDPFGAGHMG
                     ITAENIAEKYGISREEQDRFAADSQRKALAAIEAGHFKSQIVPVEIKGRKGVTLFDTD
                     EHPKAGTTAESLAGLKPAFKRDGGTVTAGNASGINDGAAACVLMEADAAARHGAKPLA
                     RLVSYGVAGVEPSLMGMGPVPAVQLALKRAGLTLDQIDVIESNEAFAAQSLGVCRGLD
                     LDPARTNPNGGAIALGHPIGASGAIIAIKCIYELMRTNKRYGLITM"
BASE COUNT          788 a         1612 c         1588 g          780 t
ORIGIN      
        1 gggcgccccc ggggacacgg ccggccagcc gccgccgaag gcggggcctc cgaagaacgc
       61 gggcgccatt gccgcctgcg gaatcatctg cgtcgacgga tgcggctgca gaccgagcgc
      121 gaccagcaca tccggcaggc cgtaacccac gcgcgccgga tcgacgttgg caagcgcacg
      181 cgcgctgtac cggatgatct ggaacaggcg ctcgacacgc tccggcccgc gcgcgccgaa
      241 cgcgccctgg aagtccggat ggtgggcaag aataaacgca gcgagcccag cgatgtgcgg
      301 cgccgccatc gaggtgccgt cccagaccgc gtaattgtcg ggcgggaccg acgaggtgac
      361 cgcgacgccc ggcgcgcaga ccgccacctc cggaccgaag cagctgaagc gggcggcata
      421 gacgccgctg cggtcgaccg gctgcgtgag tgtctgcatg tggtagctgt ccggcgggaa
      481 ctcgttgagg cgcccgattg cggcgaccgc gagcacattc ggagatgagg ccggatactg
      541 cacgcgattg ccggaattgc cggcggcggc gatgcaggcc acgccggcgc gcctggcgcg
      601 gatgatctgc tgctccagcg cctccgacgg ctgggcgccg cccaggctca ggttcacgat
      661 gtcgatctgc ttttcgatgc agtactccag cgcatcgatc agctggctga cctggccgcc
      721 cggaaacagc ttgcaagcgt ggatctcggc atccggcgcg aaaccgcgta tgccgcggcc
      781 gttgtccgcg ccggcgatgg tgccggcgca gtgcgagccg tgcgcgatca ggtcctcgtt
      841 ccaggtgtcg ggattggtgc gcttgttgat gatgtcgaag ccgaagcgga tcttgccgag
      901 atcacggtgg cgattgtcgg tgccggaatc gatcactgcc acccggatgc cctggccgcg
      961 gtactcggca ggcagctgat cgagtcgcat cgccttctgg ccccaaccat aggtctgctg
     1021 ccccggaaac gccgccagcg agggccagtc ggagatcggc cgcaggccga cgacgttcgg
     1081 ttcggacgtc gtgagtgctg gttgagcctg atagaagctc cagtagtccg ccttcggctt
     1141 cacatacagc ccgcgcaccg aactctccga ctcgccgtac agcgtgagct gcgcctggcc
     1201 gcgctggtca gtggtggcgg tggcgggcag caggctgccg aacaggtaga cttcggcgtc
     1261 gggcaccggc ttgtcgtccc ggccaagcac ggtgacgttg accgacagcg ccggtccggc
     1321 atgcgcgacc gcgcccgtca ccatgttcgg cagcgacgac gtcgtgtcga gcaggctcaa
     1381 cgactggtcg tgctcgacga tcagccgtcc ctgcgcctgc tgttgcaatg tggctgcctt
     1441 gtcgtcctcc atgcgcacca ccagcacgcc gccagggccg tcgcccagcc cgtctgcgag
     1501 cgcgccgacg atattgcgcg cgcccactgt gtcgatcacc tcgacgtcgg gactgttgcg
     1561 cagcgtgtcc tccagcatgg aaaactgcag cggctgcagt cccatcacct ggagctcgga
     1621 cgcgcggcgt ggcgcaacca ggtactgccc gcgccggctc gccacacgct tgccgccgcc
     1681 gccatgttcg acgaccgcgc ccgcgctttc cgatcggcgt gcctcatcct tcctgtcttc
     1741 gccgtccttg cgagggtcgt cgccctgact cgattcggat gcctttgcca tggtgttcct
     1801 tccgttattt acagtggacc agccacgccg ccgcacagcg gcgacagcgg ctggtccggt
     1861 tctatgaaaa tctggtgcgt cgtgcgaaac cgctcctgca gttcgcgggc ggttgcgcgc
     1921 ggcatgtgca gcacggcgat gtgcggcgca tcggccgggc cgatgaggtc caccagctcg
     1981 acgtcgtcgc gtgcgcggat ctcgtgcacg aagtcggcca ggcgccgcgc gtcctgcgca
     2041 tggacgatgt agcgcgcgcg cccgtccttg acggagtctg cagccatcgt cgatttcaat
     2101 ggatcgtcgg ctgcccttgc tgctgaccgc ccaatgccgg cgcgccaccc tggaaacccg
     2161 catatcccgg atagacggcc gcctgctgcc aatacgcgta cggatcggca ccgaacggca
     2221 gcatgcgacc cagctgtccg gccacattgc cgatcgcacc gccgagcgag ggattgccga
     2281 atgcgccgcc gatcgtggtg ccgatcggct gggccgcgcg tccgatcaga ttgccgaaga
     2341 agccctgcgg tgcgaactgc tgctgcaggt acgccgcgct catcggatcg acattgaacg
     2401 gaagaagccg gccaagctgt ccggcggcgc cgccgatcgc tccacccagg cccgcgttgc
     2461 cgaaggcgcc tccgatcgcg ctaccaaccg gctgcccgag ctggccgatc aggttgccga
     2521 agaaaccctg cggcgcaagt tgctgctgca tcgcctgctg ctgcatcgcc tgctgttcca
     2581 gcgccgcctg ctgcagcatc gccatgctca tcgggtctat cccgaaaggc agcatgcggc
     2641 cgagctgtcc gaccgcaccg ccgatcgcgc tgcccatgcc cgcattgccg aacgcgccgc
     2701 cgatcgcgcc cccgaccggt tgaccgagtt gtccgatcag gttgccgaag aagccctgcg
     2761 gcgcgaactg cggctgctgt gcgtatacgc tcatcggatc ggcgccgaac ggcagcagcg
     2821 tgccgccgat gccgccggcg gcgcggccga tctgtgcgcc caggctgggg ttgccgaaca
     2881 tgccgccgat gccgcgtccg atcatgccgc ccagcggacc gcccaacatg ccgccaagca
     2941 gaccttgtgg cgcgaactgg agaacagggc cgaagccccc cggaatgcct tgctggtagt
     3001 agtcatacat gcgatgtcct cctgcagtgg tcgaggccgc gaccggcaac gatggatgtc
     3061 cgcatgcgct gcaatccggc cgagaggttg cgtgttccgg acccgtggtg ctgaaaccgg
     3121 aagacacaat cgatgaacgc gcttgaccgg tgtgcgccgg catgcaggcc ggcatgcgcg
     3181 gcagggcggt cccgctcgtc cgctgcgcat actcgtcgct gttcctcgct tttcaaatgc
     3241 cgatatgtgg cgcgcttgag cggtctcaac gatgtgcacg ggggtgctaa gggcgcgaaa
     3301 cacgaatcgc gcacttgaga cggacacgcg cgctgcatcg gcgtaataac catctcgcgc
     3361 ggtgagacga acagtcggtg tgctcgatta aggatgggta gatgcagtga ccggtttgtc
     3421 gactactggt cgtcacgggc gctgcgccag gcacgcactt gatcgtcatg caagccggcc
     3481 gctgcgagcg ctttcccgtg gagtaccgta gtttttcgag ggtgcgtgtt gcattgcact
     3541 atgaagctgg caaaaagagt atcctttcag ctcgcacgcg aacggacgtg cgcttttttc
     3601 caattctcaa gagcgagagg caagcaagat gacaacgaga gacgtccttt tcgtcggtgc
     3661 ggcgcgcacc gccatcggca ccttcggcgg gtcgctgaag gatttcagcg cctgcgacct
     3721 tggcaccatc gccgtgaagg aagcattcaa ccgcgcgggc gtcgatccgg cggcggccgg
     3781 acagatcgtg ctcggcaacg tgatccactg cgagccgcgc gacatgtatg tctcgcgcgt
     3841 ggtcggcatc aatgccggca tggccaagga atccgccgcg ctcacgctca accgcctgtg
     3901 cggctccggg ctgcaggcga tcgtgacggc tgccaacgcg atccagcttg gcgacaccga
     3961 catcgccgtc ggcggcggcg cagaaacgat gagccgcgcg ccgtattcca ctcaggccgc
     4021 gcgcttcggc cagcgcatgg gcgatttcaa gatgatcgac atgatggtgg gcgcgctcac
     4081 cgatccgttc ggcgccggcc acatgggcat cacggcggag aacatcgccg agaagtacgg
     4141 catctcgcgc gaggagcagg accgcttcgc cgccgacagc cagcgcaagg cgctcgcggc
     4201 gatcgaggcc ggccacttca agtcgcagat cgtgccggtc gagatcaagg gcaggaaggg
     4261 cgtcacgctg ttcgacaccg acgagcatcc gaaggccggc accaccgccg agtcgctggc
     4321 tggtctgaag cccgcgttca agcgcgacgg cggcacggtc acggccggca atgcgtccgg
     4381 catcaatgac ggcgcggcgg cctgcgtgct gatggaagcg gacgccgccg cccgccacgg
     4441 cgccaagccg ctggctcgtc tcgtgtccta cggcgtcgcc ggggtcgagc cgtcgctgat
     4501 gggcatgggc ccggtgccgg ccgtgcagct ggcattgaag cgcgccggcc tcacgctcga
     4561 ccagatcgac gtgatcgaat ccaacgaagc tttcgccgcg caatcgcttg gcgtgtgccg
     4621 cggactggat ctcgatccgg cgcgcaccaa cccgaacggc ggcgcgatcg cgctcggcca
     4681 cccgattggc gcatccggcg cgatcatcgc gatcaagtgc atctacgaac tgatgcgcac
     4741 caacaagcgc tatggcctga tcacgatg
//
LOCUS       DATLGF010000173         3694 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_133669, whole genome shotgun sequence.
ACCESSION   DATLGF010000173 DATLGF010000000
VERSION     DATLGF010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133669"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VM406_05325"
     CDS             complement(<1..461)
                     /locus_tag="VM406_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HVL75416.1"
                     /translation="MTQPTSAPGTPVHGSPLEAYVTAAAVPLAIYGFPLVESIRTCRV
                     QTSVAQVTGYGRAPVNALSASERQWTHEDRDIVTPANDLLYFCGWINLADGPVTLSIP
                     RRAEADRYFVVELLDAYTDNFLNLGLRNVGAEGGRFRLVGPGMPEFARKES"
     gene            complement(532..1338)
                     /locus_tag="VM406_05330"
     CDS             complement(532..1338)
                     /locus_tag="VM406_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HVL75417.1"
                     /translation="MNKPVETYIAEQREIDRSFGGLLAKGFALLRCFIDEPRPMGNSE
                     LSQRLQLPRATVSRLCRTLYQLGYLDWDPRMDKYFVGAQVVSLGYPYLNGLAVRRIAR
                     PLMQVLADRIEGAVSLGVAHRLDVTYLETAMHPDSAPAKPDVGATRSVLTTAMGRAFL
                     ATLSKSELQHMLDAAQQERPQEYAQCIDAIRDNIANYPKRGFAINEGDAGAGVHGVGV
                     YSKIEYLNRRMLFNCALPGTRVKRGMLKKEIAPQLMEMVRAIERATGVAR"
     gene            complement(1698..1943)
                     /locus_tag="VM406_05335"
     CDS             complement(1698..1943)
                     /locus_tag="VM406_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75418.1"
                     /translation="MSTNTATLIVAENAAAANANAELIARLVARGKEYAAAFAAAMRE
                     LLTKAEGNESELRKLYRLSAASDSVSPELAETLRGGK"
     gene            complement(2102..2233)
                     /locus_tag="VM406_05340"
     CDS             complement(2102..2233)
                     /locus_tag="VM406_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75419.1"
                     /translation="MLRQLLEAMDNWLVLDPDGRGQVWFMPPALLEELPPGLIEDQI"
     gene            2677..3285
                     /locus_tag="VM406_05345"
     CDS             2677..3285
                     /locus_tag="VM406_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVL75420.1"
                     /translation="MAGPSIEFWQSRFESGSTPWDRGGPNPQLQRWLDEGAVGAQDHV
                     LVPGCGRGWEVAALAEHGLRVTGIDYAPAAVQACRTLLQARGLQAQLCEADVLEWAPD
                     APVDVIYEQTCLCALHPDHWQRYAAQLHAWLKPGGRLLALFAQALSEEAGQGFVSGPP
                     YHCDIHAMRALFPATQWQWPKPPYPRVATGPAAELAVVLQRT"
     gene            3385..>3694
                     /locus_tag="VM406_05350"
     CDS             3385..>3694
                     /locus_tag="VM406_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75421.1"
                     /translation="MAYPAPRTLTVALLSALLVACGGGGDNNASHTTGNEVGSRQNAG
                     LNDAPDSGAPPQPATGEPAPVPAPVTAFSATLASAPPQGATINAPVRIEVHGSALENV
                     E"
BASE COUNT          662 a         1196 c         1172 g          664 t
ORIGIN      
        1 tctgattcct tgcgcgcgaa ctcgggcatg ccggggccga caagccggaa gcgaccacct
       61 tccgcgccaa cattgcgcag cccgagattg aggaagttgt cggtataggc gtcgagcagc
      121 tcgacgacga aatagcggtc ggcctcggcg cggcggggaa tcgacagcgt caccggcccg
      181 tcggccaggt tgatccagcc gcagaagtac agcaggtcat tggccggggt gacgatgtcg
      241 cgatcctcgt gcgtccactg gcgctcgctc gccgacagcg cattcaccgg tgcgcggcca
      301 tagccggtta cctgcgcgac cgaggtctgc acgcggcagg tgcggatcga ttcgaccagc
      361 ggaaagccgt atatggccag cggaacggcc gccgccgtca cataggcctc gaggggcgag
      421 ccgtgcacgg gcgtgccggg cgccgacgtc ggttgcgtca tgggttgtct cctctgttgt
      481 cctcagggtt cggcggacag cgctcttcgt cgccgttccg ccttgcttac ttcatcgtgc
      541 gactcccgtg gcgcgttcga tcgcgcgcac catttccatc agttgcggcg cgatctcctt
      601 cttcagcatc ccgcgcttga cgcgcgtgcc ggggagcgcg cagttgaaca acatgcgccg
      661 gttcaggtac tcgatcttcg aatacacgcc cacaccgtgt acgccggcgc ccgcgtcacc
      721 ttcattgatc gcgaagcccc gcttcggata gttcgcgatg ttgtcgcgga tcgcgtctat
      781 gcattgcgcg tattcctgcg gccgctcctg ctgcgcggcg tcgagcatgt gctgcagctc
      841 ggacttggac agcgtcgcga ggaaggcccg tcccatcgcc gtggtcagca ccgagcgtgt
      901 cgcgccgaca tccggctttg ccggcgcgct gtccggatgc atcgcggtct cgaggtaggt
      961 gacatcgagc cggtgggcga cgcccaggga cactgcgcct tcgatccggt ccgccagcac
     1021 ctgcatcagc gggcgcgcga tgcgccgcac cgccagccca ttcaggtacg gatagccgag
     1081 tgagacgacc tgggcgccga cgaagtactt gtccatgcgc gggtcccagt cgagatagcc
     1141 gagctggtac agcgtgcggc atagccgcga gaccgtcgcg cgcggcagct gcagccgctg
     1201 cgagagctcg ctgttgccca tcggccgcgg ctcgtcgatg aagcagcgca gcagcgcaaa
     1261 gcccttggcg agcaggccgc cgaaggagcg gtcgatctcg cgctgttcgg cgatgtaggt
     1321 ttcgacgggt ttgttcatgg cagtcggatc gagtgtggaa gcggtgggcg ggcgtgtcaa
     1381 aacttggaag gcattcccgc atattggaat tccgatagct gaataatcgc gtttcgcgcg
     1441 catcgcgacc acgtgccgat ctagttgttt gcgcatgctc aacatgccgg gccctgaact
     1501 gccccatcat ggtggactga taacagcaag acgggcccag cctgctccct atcttctcga
     1561 accggcctcg cgccggtttt tcttttccgg gccgcgccct ccccctccgg gggtgctccc
     1621 gcagcgcggt gcgtgcaaaa aagaaaaggc ccctgccatt gccggcaggg gccttttttg
     1681 tggggcggac ggtgggctta cttgccgccg cgcagcgttt ccgctagttc cggagaaacc
     1741 gaatcgctgg ccgcgctcag gcggtacagc ttgcgcagtt cggattcgtt gccctccgcc
     1801 ttggtcagca actcgcgcat cgctgcggcg aatgcggcgg catattcctt gccgcgcgca
     1861 acgagacggg cgatcagttc ggcattggcg ttggcggcgg cggcattttc agcgacgatc
     1921 agcgtggcgg tattggtcga catggcattt tcctctcgaa acgttttcct gtacgagaag
     1981 tattgcgcac cgcaagatat aaatccaatt ccaatttctg ataatcgcca ttcataccaa
     2041 aaatgtctca acctgcctta cggcaggccg ttctaccctg ccgcaatccc ggtgattccc
     2101 cctatatctg atcctcgatc agccctggcg gcagttcttc cagcaaggcc ggcggcatga
     2161 accatacctg gccacggccg tccgggtcga ggacgagcca gttgtccatc gcttccaata
     2221 actgacgcag catgtttaac ctcccatcgt gagtgagcag gaccgtcggt gaacgctccg
     2281 ttgcttgcaa tcaccggacc gcaccgaggg gcgcttccac accattatgg tgcgttgcac
     2341 aacgcgtgct actgctacga cccggcggca atggaccggt tcaatccgcg cccggaagat
     2401 tgcggtcgct taagcaagcc ccattcactg cgatgctgtg gacgggatga gacagcagag
     2461 aggatgccca cacctgctgt gggcagacca gtggataacc gggcggaaag aatgacaagt
     2521 gcttgaaagc atggagaaat tttccgttga tgaaaatcaa ggcaggtgtg gaccggagcc
     2581 gggctccggc tctcgcgcgg gggcggccgc cagatgcagg acgagtcctg aaaagagggg
     2641 catcattcac ccacaaccac acgaggagac acccccatgg caggtccaag catcgagttc
     2701 tggcagtccc gtttcgagtc cggcagcacg ccctgggatc gcggcggccc caatccgcag
     2761 ttgcagcgat ggctcgacga aggcgcagtc ggcgcgcagg accatgtgct cgtaccggga
     2821 tgcggccgcg gctgggaagt ggctgcgctg gccgagcacg gcctgcgcgt gaccggcatc
     2881 gactacgcgc cggcagccgt ccaggcctgc cgcacgctgt tgcaggcacg cggtctgcaa
     2941 gcgcagctat gcgaggcgga cgtactcgaa tgggcgccgg atgcgccggt ggatgtcatt
     3001 tacgagcaga cctgcctgtg cgccctccat cccgaccact ggcaacgcta cgcggcgcag
     3061 ctgcacgcct ggctcaagcc gggaggcagg ctgctggcgc tgttcgcgca agcgctctca
     3121 gaggaagcgg gccagggctt cgtcagcggt ccgccctacc actgcgacat ccatgcgatg
     3181 cgcgcactct ttccggccac gcagtggcag tggccgaagc cgccctatcc gcgcgtggcg
     3241 acaggtcccg ccgccgaact ggccgtggtc ctgcagagaa cctaagtcac gcatgatgta
     3301 ccgcagaagt tacatttcgg gaaccgctac gctggttcca taagagatac atcgcaggga
     3361 gctgacgaat gaaacaggtg aaacttggca taccctgccc cgagaacact taccgtagcc
     3421 ctgctttccg cactgctggt cgcgtgcggt ggcggcggtg acaacaacgc ttcccacact
     3481 accggcaatg aggtcggctc ccgccagaac gcgggcctga acgacgcgcc cgacagcggc
     3541 gcgccgcccc agcccgcgac gggagaaccg gcaccagttc cggcaccggt cacggcgttt
     3601 tccgccaccc tcgccagcgc tccgccgcag ggcgcgacaa tcaacgcacc agtgcgcatc
     3661 gaagtgcatg gcagcgccct ggagaacgtc gaac
//
LOCUS       DATLGF010000174         5196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_39025,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000174 DATLGF010000000
VERSION     DATLGF010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39025"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1838)
                     /locus_tag="VM406_05355"
     CDS             complement(<1..1838)
                     /locus_tag="VM406_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVL75422.1"
                     /translation="MQPPLVTLPPPAGQAGVPALIDLSRDAILVMDAQRRIVYWSGGA
                     ARMFLYGIEEAVGSDCMHLLGFSHGGPFEAALEQTLAQGHWEGELVGYRKDGMQVVGL
                     SRWQRVGDPGPCGHILISSTDIRLWDRFLFNTARGWLQNFDALFGQHPDGVVALDPHG
                     NVLSVNPALCALTGYTAEELRALALHDLVCAEDAHVLRSAFFRARAGAPQTCDFTCRR
                     RDGGAVDASVSLLPNMIAHHVVGVHGFIKDISERKRNEARILYLANHDALTGLPNRNL
                     LFDRMQHAIEQARRQRTEIAVLFMDLNRFKIINDSLGHDKGDLLLCRVAQRLRGTLRD
                     VDTVARLGGDEFVVMLEHIRDAAQVQQVAATLLEVVARPINLEGHNLRVTTSIGASLF
                     PCDGTDPVTLLKSADLAMYAAKENGPGEFRFFESRMNDTALARLTRENNLHQAIERRE
                     FVLHYQPRLDVDTHAIVGLEALARWDHPTKGLIYPTHFIGLAEETGMIEALGEWVLRE
                     ACRQLKEWIDLGLPSIKVSVNISPHQLQSGRLCATVAKVLAETGLDAELLELEITESS
                     LMQDLGASAKALSEFQRMGISLAIDDFGTGYSSLSYLKRLPIDTLK"
     gene            2032..>5196
                     /locus_tag="VM406_05360"
     CDS             2032..>5196
                     /locus_tag="VM406_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HVL75423.1"
                     /translation="MNHPPASPLRHDKRSVRSSLDIPVVGLGASAGGLEALAQLLQGL
                     PSSPGMAFVVVLHLSPRHESAAAEVLQRSTAMPVAMVSRTTAIEPDHVYVIPPNRNLS
                     MKDGYLSLADFDRPHGPRTAIDMFFRTLADAHGERALAVVLSGSGSDGAVGIASIKEK
                     GGVTLVQHPGEAQHDGMPLAAIATGAVDFILPVAEMPQKLADLWQTARSIVLPPLEDR
                     GTDEPEAARVDVSQDAEEALQKIIALLRVHTGHDFRHYKRPTVLRRIERRMQVRGTPT
                     LPAYVELLESDANEHAALLRDMLIGVTNFFRDREAFEALQHRALAELLASKHAGEAVR
                     VWVPACSTGEEAYSLAIALADQAALLNKPPAIQIFASDIDERAISTARSALYPSSIIA
                     DVSPERLQQYFTKEDDRYRIRKSIRDQVLFAPHNVLRDPPFSKLDLISCRNLLIYLNR
                     EMQTRILEMFHFSLNPGGYLFLGSSESADLASNLFAAIDKKNRIYQAKSLSRGRLAPA
                     LPSYLQPRHHQVDSMPAPSKRHFSFAEVHQRVLAQFAPPSVIVNHDSEIVYMSDRVGR
                     FLRHVGGEPSRNLVALVHPELRVELRTALFQAVRSGKSVEARRVRLSRDGRLFFVNMT
                     VRPFHDEDAGADLVLVLFDEVEQTMSDEMASGGEKKDAVLMQLEEELQRTKEQLRETI
                     EQSEVSNEELRASNEELQAINEELRSATEELETSKEELQSVNEELSTVNYELKSKVEE
                     TGKVNDDLNNLIASADIATIFVDRGMRIKRYTPRASDIFNIIPTDIGRSLLDITHRLN
                     YPELAGDAASTFDKLRPVEREVRSLEGRYYIVRLLPYRTTEDHIEGAVMTFFDITRRR
                     EAEDQARAGEERMRLVAESVEDYAIITLDGEGRITSWSKGAERIYGHAEADAIGMPGD
                     AILAPEDGPPAGFAEEMQRARETGRVESEGWTRRKDGSRFFATGVMVPFSAGGREGFA
                     NIVRDDTARVELESRRAEELSNEHSNRELAEMANALKDEFMAIMSHELRHPLNLIHMN
                     VELLSRLDELRKTPLGVRAVNSIRA"
BASE COUNT          927 a         1711 c         1701 g          857 t
ORIGIN      
        1 atcttcaagg tatcgatcgg caggcgcttg aggtagctga gcgacgaata accggtgccg
       61 aagtcgtcga tcgcgagtga aatccccatg cgttggaatt ccgacagcgc cttggccgag
      121 gcgccgagat cctgcatcag gctgctttcg gtgatctcga gctcgagcag ttcggcatcg
      181 aggccggtct cggcgagcac ctttgccacg gtcgcgcaca gccggcccga ttgcagctga
      241 tgcggcgaga tgttcaccga caccttgatc gagggcagac ccaggtctat ccattccttc
      301 aactgccggc aggcctcgcg cagcacccat tcaccgagcg cctcgatcat cccggtttcc
      361 tcggccaggc cgatgaagtg ggtcgggtag atcaggccct tggtcggatg gtcccagcgc
      421 gccagcgctt ccagcccgac gatcgcgtgc gtgtccacgt ccaggcgcgg ctggtagtgc
      481 agcacgaact cgcgccgctc gatggcctgg tgcagattgt tctcgcgggt caggcgcgca
      541 agcgccgtgt cgttcatgcg cgattcgaaa aaacggaact cccccgggcc gttctccttg
      601 gcggcgtaca tcgccagatc ggcactcttc aaaagcgtga ccgggtcggt gccgtcgcaa
      661 gggaaaaggc ttgcgcctat gctggtcgtc acccgcaggt tgtgtccttc caggttgatc
      721 ggcctggcca ccacctccag cagcgtcgcg gccacctgct gcacctgggc ggcgtcgcgg
      781 atgtgctcca gcatgaccac gaactcgtcg ccgcccaggc gcgccaccgt gtcgacgtcg
      841 cgcaaggtcc cgcgcagccg ctgcgcgacc cggcacaaca gcagatcgcc cttgtcgtgg
      901 ccgaggctgt cgttgatgat cttgaagcgg ttcaggtcca tgaacagcac cgcgatttcg
      961 gtgcgctggc ggcgcgcctg ttcgatcgca tgctgcatgc ggtcgaacag caggttgcga
     1021 ttgggcagcc cggtcaaggc atcgtgattg gccagataga gaatgcgcgc ctcgttgcgc
     1081 ttgcgctcgc tgatgtcctt gatgaagccg tgcacgccca ccacgtggtg ggcgatcatg
     1141 ttcggcagca ggctgacgct ggcgtcgacc gcgccgccgt cccggcggcg gcaggtgaaa
     1201 tcgcaggtct gcggggcccc ggcccgcgcg cggaagaacg ccgagcgcag cacatgtgcg
     1261 tcttctgcgc agaccaggtc gtgcagcgcc agcgcccgca gttcctccgc ggtgtaaccg
     1321 gtcagtgcgc aaagggccgg gttgaccgaa agcacattac cgtgcgggtc gagcgccacc
     1381 acgccgtcgg gatgctgccc gaacagcgcg tcgaaattct gcagccagcc gcgcgcggta
     1441 ttgaacagga agcgatccca caggcggatg tcggtactcg agatcaggat gtggccgcac
     1501 ggtcccggat cgccgacccg ctgccaccgg ctcagcccca cgacctgcat gccgtccttc
     1561 ctgtagccga ccagctcgcc ttcccagtgg ccctgcgcca gcgtctgctc gagcgccgcc
     1621 tcgaacggcc cgccatggct gaagccgagc agatgcatgc agtcgctgcc gacggcttcc
     1681 tcaatgccgt acaggaacat gcgcgcggcg ccgccgctcc agtacacgat gcgccgctgc
     1741 gcatccatca cgaggatcgc gtcgcgggac agatcgatca gtgcgggaac gccggcctgc
     1801 ccggccggcg gtgggagggt cacgagtggc ggctgcattg ttagctgccc ctcgtgtggt
     1861 ggatggtggt cctgttctgc gcccgtcgat ctgtccatgt cccgtagaag tggctgcagc
     1921 cctcctgcgg gggccaacag caaattgatc gcaaggcgac gcaacaattc cacgtccgcc
     1981 cccccggaaa tgacctaaac tgcatgccgc tttgaccata acgtacccgc gatgaaccat
     2041 ccgcctgcct caccgcttcg ccatgacaag cgatccgtcc gcagcagcct cgacatcccg
     2101 gtggtcggat taggcgcatc ggccggcgga ctcgaagccc tggcgcaact cctgcagggc
     2161 cttccgtcca gtccggggat ggccttcgtg gtggtgctgc acctgtcgcc gcgccatgaa
     2221 agtgcggcgg ccgaagtgct gcagcgctcg accgcgatgc cggtcgcgat ggtttccagg
     2281 acgacagcga tcgagcccga tcatgtttac gtgatcccgc cgaacaggaa cctgtcgatg
     2341 aaggacggct atctgagtct ggccgatttc gaccgtcctc acggcccgcg cacggcgatc
     2401 gacatgttct ttcgcacgct tgcggatgcg catggcgagc gcgcgctggc ggtcgtgctg
     2461 tccggcagcg gctccgatgg cgccgtcggc atcgccagca tcaaggaaaa aggcggcgtg
     2521 acgctggtcc agcatccggg cgaagcgcag cacgatggca tgccgctggc ggccattgcc
     2581 accggcgcag tggacttcat cctgccggtg gcggagatgc cgcaaaagct ggcggacctg
     2641 tggcagacgg ccaggtcgat cgtcctgccg ccgctggagg accggggcac cgacgaaccg
     2701 gaagccgcgc gggtcgatgt cagccaggac gccgaagaag cgctgcagaa gatcatcgcg
     2761 ctgctgcgcg tgcacaccgg ccacgatttc cgccactaca agcgcccgac cgtcttgcgc
     2821 cgcatcgagc gccggatgca ggtgcgcggc acgcccacgc tgccggccta tgtcgagctg
     2881 ctggaatcgg atgccaacga gcacgcggcg ctgctgcgcg acatgttgat cggggtcacg
     2941 aacttcttcc gcgaccggga ggccttcgaa gctctccagc accgggcgct ggccgagttg
     3001 ctcgcctcca agcatgcggg cgaggcggtg cgggtgtggg tgccggcctg ctcgaccggc
     3061 gaggaagcgt attcgctcgc gatcgcgctg gccgaccagg ctgcgctgct gaacaagccg
     3121 ccggcgatcc agatattcgc gtcggacatc gacgaacggg caatcagcac cgcccgcagc
     3181 gcactctatc cgtcctcgat catcgccgac gtctcgccgg agcggctgca gcagtatttc
     3241 acgaaggaag acgaccgcta ccgtatccgc aagtcgatcc gcgaccaggt cctgttcgct
     3301 ccgcacaacg tgctgcgcga cccgccgttc tccaagctcg atctgatttc ctgccgcaac
     3361 ctgctgatct acctgaaccg cgagatgcag acccgcatcc tcgagatgtt ccatttctcg
     3421 ctgaatccgg gcggctacct gtttctcggc agctccgagt cggcggacct ggcgtcgaac
     3481 ctgtttgcag cgatcgacaa aaaaaatcgc atctaccagg ccaagtcgct ctcgcgcggg
     3541 cgcctcgcgc cggcgctgcc aagctatctg cagccgcgcc accatcaggt cgacagcatg
     3601 ccggcgccga gcaagcgcca cttctcgttc gccgaagtgc accagcgcgt gctcgcccag
     3661 ttcgcgccgc cgtcggtgat cgtcaatcac gattccgaga tcgtctacat gtccgaccgt
     3721 gtcgggcgct tcctgcgcca cgtgggcggc gagccctcgc gcaacctcgt cgcgctggtg
     3781 catccggagc tgcgcgtgga gctgcgcacc gcgctgttcc aggcagtgcg cagcggcaag
     3841 agcgtggagg cacggcgggt gcgcctgagc cgggacgggc gcctgttctt cgtcaacatg
     3901 acggtgcggc cgttccacga cgaggatgcg ggcgcggacc tggtgctggt gctgttcgac
     3961 gaagtcgagc agacgatgag tgacgagatg gcgtcgggag gcgagaagaa ggatgcggtg
     4021 ctgatgcagc tggaggaaga gctgcagcgc acgaaggagc agcttcggga gacgattgag
     4081 cagtcggaag tatcgaacga ggagctgcgc gcttccaatg aggagctgca ggcgatcaac
     4141 gaggaactgc gctcggccac cgaggagctg gaaaccagca aggaagagct gcagtcggtc
     4201 aacgaggagc tgagcacggt caattacgag ctcaagtcca aggtcgagga aaccggcaag
     4261 gtcaacgacg acctgaacaa cctgatcgcc tcggccgaca tcgccacgat cttcgtcgac
     4321 cgcggcatgc gcatcaagcg ctacacgccg cgcgcgtccg acatcttcaa catcattccc
     4381 accgacatcg gtcgttcgct gctggacatc acgcaccggc tgaattaccc ggagctggcc
     4441 ggcgatgcgg cctccacctt cgacaagctg cgtccggtcg agcgcgaggt gcgcagcctc
     4501 gaagggcgct actacatcgt gcgactgttg ccataccgca ctaccgaaga tcacattgaa
     4561 ggcgcggtga tgaccttctt cgacatcacc cggcgccgcg aagccgagga tcaggcgcgc
     4621 gccggcgagg agcgcatgcg gctcgtcgcc gagagcgtcg aggattacgc gatcatcacg
     4681 ctcgacggcg aaggccgcat taccagctgg agcaagggcg ccgagcgcat ctacgggcat
     4741 gcggaggcgg acgcaatcgg catgcccggc gacgcgatcc tggcaccgga agacggccct
     4801 cccgccggtt ttgccgagga gatgcagcgc gcgcgcgaga ccgggcgcgt ggaaagcgag
     4861 ggctggaccc ggcgcaagga cggcagccgc ttcttcgcca ccggcgtgat ggtgcctttt
     4921 tccgccggcg gccgggaagg cttcgccaat atcgtgcgcg acgacaccgc gcgggtggaa
     4981 ctggagagcc ggcgcgcgga ggaactgtcg aacgagcatt cgaaccgcga actggcggaa
     5041 atggcgaacg cgctgaaaga cgagttcatg gcgatcatgt cgcatgaact gcgccacccg
     5101 ctgaacctga tccacatgaa cgtggaattg ctgtcccggc tggatgagct gcgcaagacg
     5161 ccgctgggcg tgcgtgcggt caacagcatc cgcgct
//
LOCUS       DATLGF010000175         8218 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_53966,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000175 DATLGF010000000
VERSION     DATLGF010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 8218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8218
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53966"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VM406_05365"
     CDS             complement(<1..428)
                     /locus_tag="VM406_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVL75424.1"
                     /translation="MNRNIGYAIALVVALAAPMFLYPVLLMKVLCFALFACAFNLLIG
                     FTGLLSFGHAAFFGGAGYVAGHALKVWGVPFELGILIATATAAMLGLIVGGLAIRRQG
                     IYFTMITLALAQMLYFFCLQVPFTGGEDGLQGVPRGKLFGV"
     gene            complement(446..1330)
                     /locus_tag="VM406_05370"
     CDS             complement(446..1330)
                     /locus_tag="VM406_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVL75425.1"
                     /translation="MDIFGIPHQALLSQLLLGLVNGSFYAMLSLGLAVIFGLLNVINF
                     AHGALYMMGAFLAWMGLNYFGLNYWVMLILAPILVGIFGIVIEKTMLQWLYKLDHLYG
                     LLLTFGITLMIEGLFRSFYGVSGQPYSVPEQMLGAFDLGFMIMPKYRAWVVVASIAVC
                     LATWYMIEKTKLGAYLRAGTENPRLVEAFGINVPLMVTLTYGFGVALAAFAGVLAAPI
                     LQVSPLMGSNLIIVVFAVVVIGGMGSILGSIVTGLGLGVVEGFTRVFYPELSATVVFI
                     IMAIVLMLRPAGLFGKEK"
     gene            complement(1436..2650)
                     /locus_tag="VM406_05375"
     CDS             complement(1436..2650)
                     /locus_tag="VM406_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL75426.1"
                     /translation="MKLKAKALTAAVAGALGLGLAAPASAQISGDVIRIGFITDISGL
                     YSDIDGNGGAEAIRMAIADMGGQINGKKIELLVADHQNKADIAASKAREWMDQNGLDM
                     LIGGTNSGTSLAMAKVAQEKKRPFISIGAATSRLTNEECSPYTVHYAYDTVALARGTG
                     GAIVKQGGKDWYFLTADYAFGASLEKDTAEVVKAAGGRVVGSVKHPLSASDFSSFLLQ
                     AQSSKAQILGLANAGGDTINSIKAANEFGITRSMKLAGLLMFINDVHSLGLQATQGMY
                     LTDGWYWDQSDESRKWAKRFQEKMNRMPSMLQAADYSATMHYLNAVKALGTDDPDKVM
                     EQMRKTKINDMFAKNGVIRPDGRMVYDMYLMEVKKPSESKGPWDYYKVAQVIPGDQAY
                     TTKAESKCALWK"
     gene            complement(2712..3422)
                     /locus_tag="VM406_05380"
     CDS             complement(2712..3422)
                     /locus_tag="VM406_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL75427.1"
                     /translation="MATPALEIRDLQAWYGESHILHNVNLSVQPGEVVTLLGRNGAGR
                     TTTLRAIMGLTGTRTGSIKINGTEAINLATHKIAHLGVGYCPEERGIFSSLSAEENLL
                     LPPVVSRTEKGMTIAEIYEMFPNLEERRNSQGTRLSGGEQQMLAVARILRTGARLLLL
                     DEISEGLAPVIVQALARMITTLKAKGYTIVMVEQNFRFAAPLADRFYVMEHGQIVETF
                     ASSELEAKMPVLNELLGV"
     gene            complement(3488..4276)
                     /locus_tag="VM406_05385"
     CDS             complement(3488..4276)
                     /locus_tag="VM406_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL75428.1"
                     /translation="MASEVKGSEVILETRNLTKGFKGFIAVNDVNLQVKRGSIHALIG
                     PNGAGKTTVFNLLTKFLTPTSGSIHYNGRDITSMKPEQIAHLGVIRSFQISAVFPHLT
                     VLENVRIGLQRKLGTSFHFWKSERTLHQLNDRVMELLAEVDLSDFADTVTVDLPYGRK
                     RALEIATTLAMEPELMLLDEPTQGMGHEDVHRVTELIKKVSAGRTILMVEHNMSVVAG
                     ICDRISVLQRGSVLAEGTYAEVSSNPQVMEAYMGTTSGELVGAH"
     gene            complement(4582..5319)
                     /gene="fabG"
                     /locus_tag="VM406_05390"
     CDS             complement(4582..5319)
                     /gene="fabG"
                     /locus_tag="VM406_05390"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HVL75429.1"
                     /translation="MRLKNKVTLITGASRGIGKATALRFAREGAKVVVCDLDDAVAET
                     VAEIRLRGGNATGFTVDVTKPQSIEAMVSGVLATYGRIDVLVNNAGIVADAQLRKMTE
                     EQFDRVIDVNLKGTYNCTRAVVDAMVEKKGGVVLNASSIVGLYGNFGQTNYAASKFGV
                     IGMVKTWARELGRHGIRVNAVCPGFIETSILKSMPEKVVRALVERVPLGRLGKPDEIA
                     STFAFLASDDASYINGAVIEVSGGMTL"
     gene            complement(5594..7027)
                     /locus_tag="VM406_05395"
     CDS             complement(5594..7027)
                     /locus_tag="VM406_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HVL75430.1"
                     /translation="MKTAVGAHWDADHMLQAGMQSLLEMFDDSCEGTIAVDDQGRIAW
                     INDKYVTFLGLKNADEALGRPVEEVIPASRMREVLHTGKPILLDIMMTREQPLVVMRI
                     PVKDEQGVVIGALGFALYDRLQRLKPLVSKFAELQWALDNTKKELAHLRHTKYSISSF
                     IGVSPASMELKHRARRAAQLDTTVLLLGETGSGKELLAHGIHAASGRAGKPFVGINIA
                     AVPETLLEAEFFGVAAGAYTGADRKGRDGKFKIADGGTLFLDEVGDMPLQVQAKLLRV
                     LQEQEIEPLGSNQIVKVDVRVIAATSHDLKQLVAQGKFRSDLYYRLNVLPITLPPLRE
                     RLADLDVLCEHLLEQIAARTGMPQRELTPSARAFLASYHWPGNVRELRNALEQAGMLT
                     DSISLTAEDFATILPVSPAALAAPDNMAEKGCEVPSLAVAVADLEKNLIEKALLSTGG
                     NKAGAARMLGISRATLYQKVEEYGITV"
     gene            complement(7177..7557)
                     /locus_tag="VM406_05400"
     CDS             complement(7177..7557)
                     /locus_tag="VM406_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HVL75431.1"
                     /translation="MNDRTSKAEHVVLPPIPAKRYFTIGEVSELCGVKPHVLRYWEQE
                     FTQLKPVKRRGNRRYYQHHEVLLIRRIRELLYEQGFTISGARNKLDSRIAAAEDLAAG
                     LPPLEANTVQIREELTQILNLLKP"
     gene            complement(7566..7979)
                     /locus_tag="VM406_05405"
     CDS             complement(7566..7979)
                     /locus_tag="VM406_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HVL75432.1"
                     /translation="MNDVNSAEFQSVLAADLSRAVQESKARTQAEKDLPTLTKAELAE
                     LLFEQVGLNKREAKDMVETFFDEIRNALERGEAVKLSGFGNFQLRDKPQRPGRNPKTG
                     EEIPITARRVVTFHASQKLKSMVESVSRKPVTQIA"
     gene            complement(8011..>8218)
                     /locus_tag="VM406_05410"
     CDS             complement(8011..>8218)
                     /locus_tag="VM406_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75433.1"
                     /translation="RNAACAIMQAVVLFDEYRGKGLAEGEKSLAFRFTLQDTQSTLQD
                     EKVDAAMAAFVAAVEQQHGARLRA"
BASE COUNT         1479 a         2550 c         2513 g         1676 t
ORIGIN      
        1 acgccgaaca gcttgccgcg cggcacgcct tgcaggccgt cttcaccgcc cgtgaacggc
       61 acttgcaggc agaagaagta cagcatctgc gccagcgcca gcgtgatcat cgtgaagtag
      121 atgccctggc gacggatcgc cagcccgccg acgatcaggc cgagcatagc cgccgttgcc
      181 gtggcgatca ggatgccgag ttcgaacggc acgccccaca ctttcagtgc atggccggcc
      241 acgtagccgg cgccgccgaa gaacgcggca tggccgaacg acagcagtcc ggtaaagccg
      301 atcagcaggt tgaacgcgca ggcaaacagc gcgaagcaca gcaccttcat caacagcacc
      361 gggtacagga acatcggcgc cgccaatgcc accaccagcg cgatggcata tccaatattt
      421 cgattcatca cttgagctcc tcgaatcatt tctctttacc gaacaggccg gccgggcgca
      481 gcatcaggac gatggccatg atgatgaaca cgactgtcgc cgacagctcg gggtaaaaca
      541 cgcgcgtgaa gccctcgacg acacccagcc cgaggccggt gacgatggac ccgaggatcg
      601 accccatgcc gccgatcacg acgacggcga acaccacgat gatcaggttc gatcccatca
      661 gcggggacac ttgcaggatc ggcgcggcaa gcacgccggc gaacgcggcc agcgcaacgc
      721 cgaagccata ggtcagcgtc accatcagcg gcacgttgat gccgaacgcc tccaccaggc
      781 gcgggttctc cgtgccggcg cgcagatagg cgcccagctt ggtcttctcg atcatgtacc
      841 aggtcgccag gcagacggcg atcgaagcca ccacgaccca ggcgcggtac ttcggcatga
      901 tcatgaatcc gaggtcgaat gcgcccagca tctgttcggg aaccgaatac ggctgcccgg
      961 aaacgccata gaaggagcgg aacaggcctt cgatcatcag cgtgatgccg aaggtcagaa
     1021 gcaggccgta caggtgatcc agcttgtaca gccattgcag catggtcttt tcaatcacga
     1081 ttccgaatat gccgaccagg atcggggcga ggatcagcat cacccagtag ttcagtccga
     1141 agtagttcag gcccatccac gcgaggaacg cgcccatcat gtacagcgcg ccgtgagcga
     1201 agttgatcac gttgagcaaa ccgaaaatga ctgccaggcc cagcgagagc atggcgtaga
     1261 acgagccgtt gacgaggccg agcaggagct ggctcagcaa ggcttggtgg gggatgccga
     1321 agatatccat agattcgaaa acttgagttg tcgggtacct tcatccatga ggctgaagga
     1381 aggaggcggc gatccgccgc cccccgttcc tctcacgagg agagcgtggt cctgcttact
     1441 tccagagcgc gcacttcgat tccgccttgg tcgtgtatgc ctgatcgccc gggatcacct
     1501 gagcgacctt gtagtagtcc cacgggccct tggactcgga cggcttcttc acttccatca
     1561 ggtacatgtc gtacaccatg cggccgtccg gacggatcac gccgttcttg gcgaacatgt
     1621 cgttgatctt ggtcttgcgc atctgctcca tgaccttgtc cggatcatcc gtgccgaggg
     1681 ccttgaccgc gttcaggtag tgcatggttg ccgagtagtc ggcagcctgc agcatggacg
     1741 gcatgcggtt catcttttcc tggaagcgct tggcccactt gcgcgactcg tcgctctgat
     1801 cccagtacca gccgtcggtc aggtacatgc cttgcgtggc ctgcaggccc agcgagtgca
     1861 cgtcgttgat gaacatcagc agaccggcca gcttcatgct gcgggtgatg ccgaactcgt
     1921 tggccgcctt gatcgagttg atcgtgtcgc cgccggcgtt ggccagaccg aggatctgcg
     1981 ccttcgacga ttgagcctgc agcaggaagg acgagaagtc cgatgccgac agcgggtgct
     2041 tcaccgaacc gacgacgcgg ccgccggctg ccttgacgac ttccgccgtg tccttctcga
     2101 gcgaagcgcc gaacgcgtag tcggcggtca ggaagtacca gtccttgccg ccctgcttga
     2161 cgatcgcgcc gccggtgccg cgcgccagcg caacggtgtc atatgcatag tgcacggtgt
     2221 acggcgagca ctcttcgttg gtcaggcggg aggtcgctgc gccaatggag atgaacgggc
     2281 gcttcttctc ctgcgcgacc ttggccatgg ccaggctggt accggagttg gtgccgccga
     2341 tcagcatgtc cagaccgttc tggtccatcc actcccgcgc cttggacgcg gcgatgtccg
     2401 ccttgttctg gtggtcggcc acgagcagtt cgatcttctt gccgttgatc tggccgccca
     2461 tgtcggcgat cgccatgcgg atggcttccg cgccgccgtt gccgtcgatg tccgagtaca
     2521 ggcccgagat gtcggtaatg aagccgatac ggatgacgtc gccggagatc tgcgcggatg
     2581 ccggtgcggc cagacccagg ccgagtgccc ctgcaacggc tgcggtgagt gcttttgctt
     2641 tgagtttcat ttcttctcct ctgttttgga tgaacatgcg atgacgcatc ggattgctac
     2701 tgctggtgta ctcagacgcc cagaagctcg ttcagcacgg gcatcttggc ttccagttcg
     2761 gatgaggcga aggtctcgac gatctgaccg tgctccatca cgtaaaaccg atccgccagt
     2821 ggtgcggcga agcggaaatt ctgttcgacc atcacgatcg tgtagccctt ggctttcaga
     2881 gtcgtgatca tgcgcgcgag ggcctggacg atgaccgggg ccaggccctc ggagatttcg
     2941 tccagcagca gcaggcgtgc gccggtgcgc aggatccgcg cgaccgcgag catctgctgt
     3001 tcgccgcccg acaggcgggt gccctggctg ttgcggcgtt cctcgaggtt ggggaacatc
     3061 tcgtagattt ctgcgatcgt cattcccttt tcggtacgcg agacgaccgg tggcagcagc
     3121 aggttctcct ccgcggagag cgacgagaaa atgccgcgct cttccgggca gtagccgaca
     3181 ccaaggtgag cgatcttgtg cgtggccagg ttgatcgctt cggtcccgtt gatcttgatc
     3241 gacccggtac gcgtaccggt gaggcccatg atggcgcgca gcgtcgtggt gcggccggcg
     3301 ccgttgcgtc cgagcagcgt caccacttcg cccggctgca ccgagaggtt gacgttatgc
     3361 aggatgtgcg actcgccgta ccaggcttgc aggtcgcgga tttccagcgc cggcgtggcc
     3421 atctgcgggc tgcgtccggt gctatggacg gcttggcctt ccaggccctt caacgttgct
     3481 gtcgtcatca gtgcgctcct accagttctc ccgaggtcgt gcccatatag gcctccatca
     3541 cctgcggatt gctcgatacc tcggcatagg taccctcagc cagcacggag ccgcgctgaa
     3601 gcaccgaaat gcggtcgcag atccctgcga ccacgctcat gttgtgctcc accatcagga
     3661 tggtgcgtcc ggccgacacc ttcttgatca gctccgtgac gcggtgcaca tcctcgtggc
     3721 ccatgccctg tgtcggctcg tcgagcagca tcagctccgg ctccatcgcc agcgtggtgg
     3781 cgatttcgag cgcgcgcttg cggccgtatg gcagatccac cgtgaccgtg tcggcgaagt
     3841 cggaaagatc gacttcggca agcagctcca tcacgcggtc attgagctga tgcagcgtgc
     3901 gctcgctctt ccagaaatga aacgacgtgc cgagcttgcg ctgcagaccg atgcgcacgt
     3961 tctccagcac cgtcaggtgc gggaacacgg cggagatctg aaacgaacgg atcacgccga
     4021 ggtgagcaat ctgctcaggc ttcatcgacg tgatgtcgcg gccgttgtag tggatcgagc
     4081 cggaagtcgg cgtgaggaac ttggtgagca ggttgaagac ggtggtcttg cccgcgccgt
     4141 tcggtccgat caaggcgtgg atcgatccgc gcttgacctg caggttcacg tcattcacgg
     4201 cgatgaagcc cttgaacccc ttggtcaggt tcctggtctc cagaatcacc tcacttccct
     4261 ttacttcgct tgccatcggt tttctccagc gttgccattg ttcttcgcgc ttcggccgca
     4321 tgcgtgcccg ggcgcgcgtt gcccgctccc tgcggcgtgc ggccgcaggg aaaagcttgc
     4381 gtcttcagtc cggaatgttt gctgcaataa cggcgcttcc gccgcacgcg gccagcaggc
     4441 cggccgcgca cagagagctc aatccaactg ccttgctgcc acttctcatc gctgtctcct
     4501 ttattgcgtt cctgtggaac gctatttctt cgatggtcac gcgtctcgcc atcactggcg
     4561 cttgtgcaga tcgttgaatt cctagagcgt cattcctccg ctgacttcga tgacagcgcc
     4621 gttgatgtag ctcgcgtcgt cggacgcgag aaaggcgaag gtgctggcaa tttcatcggg
     4681 cttgccgagg cgtcccagcg gcacgcgctc gaccagcgcg cgcacgacct tctcgggcat
     4741 ggacttgagg atggaggttt cgatgaatcc cgggcagacg gcgttgacgc gtatgccatg
     4801 acggccgagt tcgcgcgccc aggtcttgac catgccgatc acgccgaact tgctggcggc
     4861 atagttggtc tggccgaaat tgccgtagag gcccacgatc gaggaggcgt tcagcacgac
     4921 cccgcccttc ttctcgacca tcgcgtccac cacggcgcgg gtgcagttgt aggttccctt
     4981 gaggttgacg tcgatgacgc gatcgaactg ctcttcggtc atcttgcgca gctgcgcgtc
     5041 ggccacgatg ccggcgttat tgacgagcac gtcgatgcgg ccgtatgtcg ccagcacccc
     5101 gctgaccatc gcctcgatgc tttgaggttt tgtgacgtcg accgtaaagc cggtggcatt
     5161 gccaccgcgc agacggattt cggcgacggt ttcggccacg gcatcgtcca ggtcgcagac
     5221 cacgaccttc gccccttcgc gggcgaaccg gagtgccgtc gccttcccga ttccccgact
     5281 cgcgcctgta atcagcgtga ccttgttctt gagtcgcacg ttgtctccct catccgcgcc
     5341 gcgcctgcgc tctcaggagc gtcgcggtcg tcggtcttct agtgcgatcc aattggcaat
     5401 agctgtgcca ggtcaggcaa gtatttgatt tttcacatat ttttatcgcg gaggcggcaa
     5461 aagagcattt gccggcggat gaaagcagcg gaaatgtgta cggcatgaat acacattttg
     5521 cttatatctt cttatgtgat tgaaaaataa cgcgtttttc gacacgtctg aaattcggac
     5581 aagtgtatgg atctcataca gtgatgccgt attcctcgac cttttggtac agcgtggcgc
     5641 gggaaatccc cagcatgcgg gctgccccgg ccttgttgcc gccagtggag agcaaggcct
     5701 tttcgatgag attcttctcc agatctgcga ccgccaccgc aagggaaggc acttcgcagc
     5761 ccttttccgc catattatcg ggagcggcca gtgcggcggg cgacacggga agaatggtgg
     5821 cgaagtcttc cgcggtcagc gagatactgt cggtcagcat gccggcctgc tcaagcgcgt
     5881 tgcgcagctc gcgcacgttg ccgggccagt ggtaggacgc gagaaaagcg cgcgcggagg
     5941 gcgtaagctc acgctgcggc atgccagtgc gtgccgctat ctgctcgagc aggtgctcgc
     6001 acaggacatc gaggtccgcc aggcgctcgc gcagcggcgg cagcgtaatc ggcaacacgt
     6061 tcagccggta atagaggtcc gagcggaact tgccctgcgc gacaagctgc ttgaggtcgt
     6121 ggctggtcgc ggcaatcaca cggacatcga ccttgacgat ctggttggag cccagcggct
     6181 cgatctcctg ctcctgcagc acgcgcagca gcttcgcctg cacctgcagc ggcatgtcac
     6241 cgacttcgtc gagaaagagc gtgccgccgt cggcgatctt gaacttgccg tcccgccctt
     6301 tgcggtcggc gccggtatag gcgcctgcag ccacgccgaa aaattccgcc tccagcaagg
     6361 tctcgggcac ggccgcgata ttgattccga caaacggctt gccggcgcgt cccgacgcgg
     6421 cgtgaatgcc atgtgcgagc agttccttgc cgctgccggt ctcgcccagc agcagcaccg
     6481 tggtgtcgag ctgcgccgcg cgtcgggcac ggtgcttcag ctccatcgag gcgggactga
     6541 cgccgatgaa gctcgaaatc gaatactttg tgtggcgcag gtgcgccagc tccttcttgg
     6601 tattgtcgag cgcccactgc agctccgcga acttcgaaac cagcggcttg aggcgctgca
     6661 gcctgtcgta gagggcaaag ccgagcgcgc cgatcaccac gccctgttcg tccttgaccg
     6721 ggatgcgcat tacgaccagc ggctgctcgc gcgtcatcat gatgtccagc aggatcggct
     6781 ttccggtgtg gagcacctcg cgcatgcggc tggccgggat cacctcctcg accgggcggc
     6841 cgagcgcctc gtccgcgttc ttcagcccaa gaaacgtcac gtacttgtcg ttgatccagg
     6901 cgatgcgacc ctgatcatcg accgcgatcg tcccttcgca ggaatcgtcg aacatttcga
     6961 gcagggactg catgcccgcc tgcagcatgt ggtcggcatc ccagtgcgcc ccaactgcgg
     7021 ttttcatcgc ttcgtctcct gatcaggttc ttgttcttgc ggttttaccg ttaggcaaag
     7081 gattatagat tcttagtaga aatattgggt gcgctgctta cacgcgcgaa aaaaaagccg
     7141 cacggggcgg cttgcaacga tacggcaatg tagcgctcac ggtttgagca agttgaggat
     7201 ctgtgtcagt tcttcgcgaa tctggacagt gttcgcttcc agcgggggta ggcccgcagc
     7261 caggtcctca gcggcggcga tccggctgtc gagcttgttg cgcgcgccgc tgatcgtaaa
     7321 gccctgctcg taaagcagct cgcggatgcg ccggatcagc agcacttcat ggtgctggta
     7381 gtagcgccgg ttgccacgcc gcttgacagg cttgagctgc gtgaactcct gctcccagta
     7441 gcgcagcacg tgcggcttga cgccgcacag ctcgctcacc tcaccgatcg tgaagtaacg
     7501 cttggccgga attggcggca aaacgacgtg ctccgccttg ctggtgcggt cgttcatggt
     7561 tcagatcagg cgatctgggt aaccggcttg cggctgacgc tctcgaccat gctcttgagc
     7621 ttctggctcg catggaaggt caccacgcgc cgcgcggtga tcgggatctc ttcgccggtc
     7681 ttcggattgc gccccggacg ctgaggcttg tcgcgcagct ggaagttgcc gaaacctgat
     7741 agcttgacgg cttcgccgcg ttccagcgca ttgcggatct cgtcgaagaa ggtctcgacc
     7801 atgtccttgg cctcgcgctt gttgagcccg acctgctcga acagcagctc ggccagctcg
     7861 gccttggtca acgtggggag atccttttca gcctgcgtgc gcgccttcga ttcctgcaca
     7921 gcccggctca gatcggcggc caggacggat tgaaattcgg ctgaattcac gtcattcata
     7981 gttaggagtc ctgccatgcc ggatcacgca ctacgcacgc aaacgggcac cgtgctgctg
     8041 ttcgactgcg gcaacgaagg cggccattgc cgcgtcgact ttctcgtcct gcagggttga
     8101 ctgagtatct tgcaaggtaa accggaacgc aaggcttttt tcgccctccg cgagtccctt
     8161 cccccgatat tcatcaaata aaacaacggc ttgcatgata gcacaggcgg cgttccga
//
LOCUS       DATLGF010000176         5993 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_85904,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000176 DATLGF010000000
VERSION     DATLGF010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85904"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /gene="ychF"
                     /locus_tag="VM406_05415"
     CDS             <1..991
                     /gene="ychF"
                     /locus_tag="VM406_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HVL75434.1"
                     /translation="CTIEPNVGVVEVPDARLQQLADIVKPERIVPAIVEFVDIAGLVA
                     GASKGEGLGNQFLAHIRETDAIVNVVRCFEDPNVVHVAGKVSPLDDIAVIQTELALAD
                     LGTVEKTIQREGKKAKAGDKEAIKLVALLERIQAGLNEAKPVRALGLDADEMALIKPL
                     CLITAKPAMYVGNVSDTGFTNNPLLDQLAAYAAEQNAPLVAICAAIEAEIADLDEADK
                     GEFLADMGMDEPGLNRLIRAGFKLLGLQTYFTAGVKEVRAWTVPIGATAPQAAGVIHT
                     DFERGFIRAQTISFDDYITFKGEQGAKEAGKMRAEGKEYVVKDGDVLNFLFNV"
     gene            1008..1859
                     /locus_tag="VM406_05420"
     CDS             1008..1859
                     /locus_tag="VM406_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HVL75435.1"
                     /translation="MPTISELTLYPIKSCAGISLREATLTEAGLMSEHIYDREWMVVD
                     AQGEFLSQREHPRMALIQPKIKSETLELRAPGMLRIEVPLGLPDPEDARVIDVRVWDD
                     TVKAYDCDETTAAWFSSFLGTPCRLVRFHADARRSVDSDWTGGIPATTMFSDGFPLLV
                     ISEASLADLNEKLAGQGRARLPMNRFRPNVVVNGIGAFEEDYVQALRAGDAVLKPVKP
                     CARCTIPSVDQATGERGPDPVDVLQTYRANEKVGGGITFGMNAIVAAGEGSVLRVGQD
                     IEAELAF"
     gene            complement(1896..2153)
                     /locus_tag="VM406_05425"
     CDS             complement(1896..2153)
                     /locus_tag="VM406_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HVL75436.1"
                     /translation="MALMITDDCINCDVCEPECPNEAIYMGPEIYEIDPNKCTECVGH
                     FDEPQCQQVCPVACIPFNPAWRESREELQAKYERLQTEKQV"
     gene            complement(2167..2658)
                     /gene="coaD"
                     /locus_tag="VM406_05430"
     CDS             complement(2167..2658)
                     /gene="coaD"
                     /locus_tag="VM406_05430"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HVL75437.1"
                     /translation="MVTAVYPGTFDPLTRGHEDLVRRASGLFDKLVVGVAISTNKRPF
                     FSLDERLSIANEVLGHYPNVQVESFSGLLKDFVRAHDARVIVRGLRAVSDFEYEFQMA
                     GMNRYLLPDVETLFLTPSDQYQFISGTIVREIATLGGDVSKFVFPSVERWLKEKIAKG
                     NNT"
     gene            complement(2699..3325)
                     /gene="rsmD"
                     /locus_tag="VM406_05435"
     CDS             complement(2699..3325)
                     /gene="rsmD"
                     /locus_tag="VM406_05435"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HVL75438.1"
                     /translation="MQSRKTARAAPAAAHRIRIIGGQWKRTPLPVPDAEGLRPTPDRV
                     RETVFNWLTHLLNWDEVRCLDLFAGTGALGFEAASRGAAHVVMVEQFTPALRQLEATR
                     TKLGAARVEVRRGDAFAVAREFTAAGRRFELIFLDPPYHQDWLARMLPQCERLLTDGG
                     LVYAEAEHALDGQVAPAWMDGWDVLRSDRAGLVYYHLLRRKTVPQNAA"
     gene            complement(3326..4645)
                     /locus_tag="VM406_05440"
     CDS             complement(3326..4645)
                     /locus_tag="VM406_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HVL75439.1"
                     /translation="MFRRFLFSCLIGLIAHAPALAALKIEAWTLENGARVLFVENHTI
                     PMLDVSVDFDAGSRRDPQGKPGTALLTQAMLARGLRAEGKEPALTEAQISDTFADTAA
                     QQGGAAGMDRAGVTLRTLADPAERDVSVALLARLLAHPSFPDNFLARDRARLIAAIKE
                     EETKPEAIADKAFRRHAYGDHPYGRSPTAASVQAIARDDLVRFHRTHYVANRAVVAMI
                     GSISRAQADAIARELTRRLPQGAPLPELPQVRIAPGQEHWIAHPASQAHVVIGMPAVA
                     RGDPDFFPLMVGNYTLGGGGFVSRLMREVREKRGLAYSAYSYFSPLAQPGPFEAGLQT
                     QREQADNALGVVRETIGAFLRDGPSEQELQAAKDNLIGGFALRIDNNRKILDNIAMIG
                     FYGLPLDYLDTWTERIARVTLADVRAAFGRKLAVEGMTTVVVGGARQ"
     gene            complement(4653..5969)
                     /locus_tag="VM406_05445"
     CDS             complement(4653..5969)
                     /locus_tag="VM406_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HVL75440.1"
                     /translation="MQTIRAETAEFTLANGMKLIVREDRRAPTVAHMIWYRTGSIDEQ
                     SGVTGVAHVLEHMMFKGTKTLAPGEFSQRVAALGGRENAFTSKDYTAYFQQIEKSRLE
                     QVMALEADRMVNLVFDPEEFAREIRVVMEERRWRTDDQPLGLVHEALNATAYMAHPYR
                     HPIVGWMSDLQSMTVDDARAWYEHWYAPNNAVMVVVGDVDAGEVRALAEKHFGAIPRR
                     PVPVTRPQHEPEQRGIKRVTVKAPAENPYVALAFKAPTLRDVEKDEEVHALDVLAAIL
                     DGYDNARLPARLVRTDRVANSVGAGYSNISRGPSLFVLNGTPAAGTTTAQLEKLLRAE
                     VARVAKEGVSEQELQRVKAQLIAGQVYKRDSIFGQAMEIGIMEMSGLSYRDIDRIIEK
                     LREVTAEQVQAVARKYFHDDNLTVATLVPLPLTEKKEPAPPAGLRH"
BASE COUNT         1027 a         1961 c         1971 g         1034 t
ORIGIN      
        1 ctgcacgatc gagcccaacg tcggcgtggt cgaagtgccg gatgcgcggc tgcagcaact
       61 ggccgacatc gtcaagcctg agcgcatcgt gccggccatc gtcgagttcg tcgatatcgc
      121 cggcctggtc gccggtgcgt cgaagggcga aggcctgggc aaccagttcc tcgcccatat
      181 ccgcgaaacg gatgcgatcg tcaatgtcgt gcgctgcttc gaggatccga acgtcgtgca
      241 cgtggccggc aaggtcagcc cgctcgacga catcgcggtg atccagaccg aactcgcgct
      301 ggccgatctc ggtacggtgg aaaaaaccat ccagcgcgaa ggcaagaaag ccaaggccgg
      361 cgacaaggaa gcgatcaagc tggttgcgct gctcgagcgt atccaggctg gtctcaatga
      421 agccaaaccg gtgcgcgcac tgggactgga tgccgatgaa atggccctga tcaagccgct
      481 gtgcctgatc accgccaagc cggcgatgta tgtcggcaat gtctccgaca ccggcttcac
      541 caacaatccg ctgctggatc aactggccgc ctatgcggcc gaacagaatg cgccgctggt
      601 ggcgatctgc gcggcgatcg aagccgagat cgccgatctg gacgaagccg acaagggcga
      661 gttcctggcc gacatgggca tggacgaacc cggcctgaac cgcctgatcc gcgccggctt
      721 caagctgctc ggcctgcaga cctatttcac cgcgggcgtg aaggaagtgc gcgcctggac
      781 ggtgccgatc ggcgccactg ctccgcaagc cgccggcgtg atccacaccg atttcgagcg
      841 cggcttcatc cgcgcccaga cgatctcctt cgacgactac atcacgttca agggcgaaca
      901 gggcgcaaag gaagcaggca agatgcgcgc cgaaggcaag gagtacgtcg tcaaggatgg
      961 cgatgtgctg aacttccttt tcaacgtgtg agcgccacga tatcgccatg cccaccatct
     1021 ccgaactcac gctgtacccg atcaagtcct gtgccggcat ctcgctgcgc gaagccacgc
     1081 tcactgaagc cggcctgatg agcgaacaca tctacgaccg cgaatggatg gtggtcgacg
     1141 cccagggcga gttcctcagc cagcgcgagc atccgcgcat ggcgctgatc cagccgaaga
     1201 tcaagtcgga gacgctggag ctgcgcgcgc caggcatgct gcgcatcgag gtgccgctcg
     1261 ggctgcccga tcccgaggat gctcgcgtga tcgatgtgcg cgtctgggac gacaccgtga
     1321 aggcctacga ctgcgacgag accaccgccg cgtggttctc cagcttcctc ggcacgccct
     1381 gccggctggt gcgcttccat gcggacgcgc gccgcagcgt cgacagcgac tggaccggcg
     1441 gcataccggc gacgacgatg ttctcggacg gctttcctct gctggtgatc tcggaagcgt
     1501 cgctggccga cctcaatgaa aagcttgcgg gacaagggcg cgcgcggctg ccgatgaacc
     1561 ggttccgtcc caatgtggtc gtgaacggca tcggcgcgtt cgaagaggat tatgtgcagg
     1621 cgctgcgcgc cggcgatgcg gtcttgaagc cggtgaagcc ctgcgcgcgc tgcacgatac
     1681 cgtcggtgga tcaggccacc ggcgaacgcg ggccggatcc ggtcgacgtg ctgcagacct
     1741 accgcgccaa cgaaaaggtt ggcggcggga ttacgttcgg aatgaatgca atcgtcgcgg
     1801 caggcgaagg cagcgtgctg cgtgtggggc aggacatcga ggcggaactg gcgttctgag
     1861 ccggcgcagg ccgacggccc gtactcaggg cccggtcaaa cctgcttctc ggtctgcagc
     1921 cgctcgtact tcgcctgcag ctcctcgcgc gattcgcgcc aggccggatt gaacgggatg
     1981 cacgcgaccg gacacacctg ctggcactgc ggttcatcga agtggccgac gcattcggtg
     2041 cacttgttcg gatcgatctc gtagatctcc ggccccatgt agatggcctc gttcgggcac
     2101 tcgggctcgc acacgtcgca gttgatgcag tcgtcggtga tcatcaaggc catggtgaat
     2161 gcctgatcag gtgttgttgc ccttcgcgat cttttccttc agccagcgtt cgaccgaagg
     2221 gaacacgaat ttggatacgt ccccgcccag cgtggcgatc tcgcgcacga tggtgcccga
     2281 gatgaactgg tactggtcgg acggggtgag aaacagggtt tcgacgtccg gcagcaggta
     2341 gcggttcatg ccggccatct ggaattcata ctcgaagtcc gacacagcgc gcagcccgcg
     2401 cacgatcacg cgcgcgtcgt gtgcccgcac gaagtccttc agcaggccgg aaaagctctc
     2461 gacctgcacg ttcgggtagt gcccgaggac ttcattggcg atcgacaggc gctcgtcgag
     2521 cgagaagaac gggcgcttgt tggtgctgat cgccacgccg accaccagct tgtcgaacag
     2581 gccggaggca cggcgcacca agtcttcatg gccccgggtc agcgggtcga acgttcctgg
     2641 gtagactgct gtaaccatcg cggctccctc accaatggta ggtcaatcga acgcgccatt
     2701 atgccgcatt ttgcgggacg gttttgcgtc gcagcaagtg gtagtaaacc aggccggcgc
     2761 gatccgagcg cagcacgtcc cacccgtcca tccacgccgg cgctacttgt ccgtcgagcg
     2821 cgtgctccgc ttcagcatag accaggccgc cgtcggtcaa caggcgttca cattgaggca
     2881 acatccgcgc caaccagtcc tgatgatagg gagggtcgag aaagatcagc tcgaagcggc
     2941 ggccggccgc ggtgaattcg cgcgcgactg caaacgcgtc gccgcgccgc acctcgacgc
     3001 gggccgcgcc aagcttcgtg cgcgtggcct cgagctggcg caatgccggt gtgaactgtt
     3061 cgaccattac cacgtgcgcc gcgccgcggc tggcggcctc gaagccgagc gcaccggtgc
     3121 ccgcgaacag gtccaggcag cgcacctcgt cccagttcag cagatgcgtg agccagttga
     3181 acaccgtttc ccgcacgcgg tccggggtcg ggcgcaggcc ctcggcgtcg ggcaccggca
     3241 acggcgtgcg cttccactgg ccgccaatga tccggatgcg gtgcgccgca gccggggccg
     3301 cccgcgctgt ctttcgggat tgcatctact ggcgcgctcc gcccaccacc accgtggtca
     3361 tgccctcgac cgcgagcttg cggccgaacg cggcgcgcac gtccgcgagc gtcacgcgcg
     3421 cgatacgctc ggtccaggtg tcgaggtaat cgagcggcaa gccgtagaag ccgatcatcg
     3481 cgatgttgtc gaggatcttg cggttattgt cgatgcgcag cgcaaagcct ccgatcaggt
     3541 tgtccttggc cgcctgcagc tcctgttcgc tcggcccgtc gcgcaggaaa gcaccgatgg
     3601 tctcgcggac cacgccgagc gcattgtcgg cctgctcgcg ctgcgtctgc aggccggctt
     3661 cgaacggacc gggctgcgca agcgggctga aatagctgta cgcgctgtag gccaggccgc
     3721 gcttctcgcg cacctcgcgc atcaggcgcg agacgaatcc gccgcctccg agcgtgtagt
     3781 tgccgaccat gagcgggaag aaatccgggt cgccgcgggc gaccgccggc atgccgatga
     3841 ccacgtgcgc ctgcgaggcg ggatgcgcga tccagtgctc ctgccccggc gcgatccgca
     3901 cctgcggcag ctccggcagc ggtgcgccct gaggaagacg gcgggtgagt tcacgcgcaa
     3961 tcgcatcggc ctgggcacgc gagatcgagc ctatcatcgc aaccaccgcg cggttcgcga
     4021 cgtaatgtgt gcgatgaaaa cgcaccaggt catcccgcgc aatcgcctgc accgaggccg
     4081 ccgtcggact gcgcccgtaa gggtgatccc catacgcatg gcggcggaag gccttgtcgg
     4141 caatcgcttc aggcttggtt tcctcctcct tgatcgcggc gatcaggcgc gcgcggtcgc
     4201 gcgcaaggaa gttgtcgggg aagcttggat gggcgagcag gcgcgcgagc agcgcgacag
     4261 acacatcgcg ctcggcggga tcggccagcg tgcgcagcgt gacgcctgcg cggtccatgc
     4321 cggccgcgcc gccctgctgg gcggcggtat cggcaaaggt gtcggagatc tgggcttcgg
     4381 tgagcgccgg ctccttccct tccgcgcgca gtccgcgcgc cagcatcgcc tgcgtcagca
     4441 aggcggtgcc gggcttgcct tgcgggtcgc gccgcgaacc cgcatcgaaa tccacgctga
     4501 cgtcgagcat cggaatcgtg tgattctcga caaacagcac gcgcgcgccg ttctcgagcg
     4561 tccaggcttc gatcttgagc gcggcgaggg ccggcgcgtg ggcaatgagg ccgatcaggc
     4621 aggaaaacag aaagcgtcgg aacatggcag tctcaatggc gtaggccggc cggcggcgcc
     4681 ggctcctttt tctcggtcag gggcagcggc accagcgtgg ctacggtgag gttgtcgtca
     4741 tggaagtact tgcgcgccac cgcctggact tgctcggcgg tgacctcacg cagcttttcg
     4801 atgatgcgat cgatgtcgcg gtaagacagc ccgctcatct ccatgatgcc gatttccatc
     4861 gcctggccga agatcgagtc gcgcttgtag acctgtccgg caatcagctg cgccttcacg
     4921 cgctgcaatt cctgctcgga cacaccctcc ttggccacgc gcgcgacctc ggcgcgcagc
     4981 agcttttcca gttgcgcggt tgttgtgcct gcggcgggcg tgccgttgag cacgaacagc
     5041 gacgggccgc gcgaaatatt cgaatagccg gcaccgaccg aattggcgac gcggtcggta
     5101 cgcaccagcc gtgcaggcag acgggcgttg tcgtaaccgt cgaggatcgc ggccagcacg
     5161 tcgagcgcat gcacttcctc gtccttttcg acgtcgcgca gcgtgggtgc cttgaaggcc
     5221 agcgcgacat acgggttctc ggccggcgcc ttgaccgtca cgcgcttgat gccgcgctgc
     5281 tccggctcgt gctggggccg ggtcaccggc accgggcgtc gcggtatcgc gccgaagtgc
     5341 ttctcggcca atgcgcgcac ttcgccggcg tcgacgtccc cgaccaccac catcaccgca
     5401 ttgttcggcg cgtaccaatg ctcgtaccag gcgcgcgcgt cgtcgactgt catgctctgc
     5461 aggtcgctca tccagccgac gatagggtga cgatacggat gcgccatgta cgcagtcgcg
     5521 ttcagggcct catgcaccag tccgagcggc tggtcgtccg tgcgccagcg gcgttcctcc
     5581 atcacgacgc ggatctcacg cgcgaattct tcggggtcga acacaagatt gaccatccgg
     5641 tccgcttcca aggccatcac ctgttccagc cgcgatttct cgatttgctg gaagtaggcg
     5701 gtgtaatcct tgctggtgaa cgcgttctcg cgaccgccga gcgcggcgac gcgctggctg
     5761 aactcgccag gcgccagggt cttcgtgccc ttgaacatca tatgctcgag cacgtgcgca
     5821 acaccggtca cgccgctctg ctcgtcgatg gaaccggtgc gataccagat catgtgggcg
     5881 accgtcggtg cgcgcctgtc ctcgcgcacg atgagcttca tgccgtttgc gagcgtgaac
     5941 tcggcggttt cggcgcggat ggtctgcacg gcggcttgcg gcgcctgggc ggc
//
LOCUS       DATLGF010000177         9914 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_39202,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000177 DATLGF010000000
VERSION     DATLGF010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 9914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9914
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39202"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VM406_05450"
     CDS             <1..489
                     /locus_tag="VM406_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75441.1"
                     /translation="VFNISRDKTLAWLDFDGTSLPNGTLLARVVAFSSPPGGAGQEIE
                     VMPVRTWYLRNEPQPALNGPIPAPTTMPEAWLRLVDLPFVDPEPLAEMMRLDDASYSR
                     LMTDEPERVRQTMYRYAPANVVLFPAPLGFVGPWDSCLQQPALLACREAMNNMIGLMN
                     SK"
     gene            complement(523..1314)
                     /gene="pcaD"
                     /locus_tag="VM406_05455"
     CDS             complement(523..1314)
                     /gene="pcaD"
                     /locus_tag="VM406_05455"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HVL75442.1"
                     /translation="MPVQSGSLHYQIEGDEGAPWLILSNSLGTALEMWDPQMPALLRQ
                     FRVLRYDTRGHGRSEVTPGPYTIAQLGADVIGLMDGLDIARAHFCGLSMGGMTGMWLG
                     IHHPQRVGRLALCNTAARIGPPELWNARIAKVTSEGMASIAPAVIDRWFTPRFQQEAR
                     QQVERVRAMLESMPPDGYAACCAAVRDMDQRGALGRIQAPTLVIAGTHDGSTPPADGR
                     AVAGCIAGARYVELDAAHLSNWEQCEQFTDAVVGFLNEGAPQASA"
     gene            complement(1513..2490)
                     /locus_tag="VM406_05460"
     CDS             complement(1513..2490)
                     /locus_tag="VM406_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVL75443.1"
                     /translation="MKHALLKTALAGLALTAAFAGTAHANDAWPTKTVRWVVPFPPGG
                     AMDVIARTLGDKLSQSLKQPVIIENRPGAGGTIGSNTVAKADPDGHTIMIVSIGHALN
                     PNLYPRLPYDAAKDFEPVSLVAIVPNVLVVNSTVKANSVSELVAQAKANPGKLTYASA
                     GNGTSIHMAGEMFNSIAGVDIMHVPYKGSGPAVTDLIGGQVNMMFDSITSAKPHIESG
                     RLRPLAVTTAKRSATLPNVPTAAEAGLKGYEVSPWFAVFAPANTPKPVIDRLHGEIVK
                     AMQMPDVKARFATIGAEPVGSSPEELRTYLRSETDRWAKLIKERNITAN"
     gene            complement(2519..2797)
                     /gene="catC"
                     /locus_tag="VM406_05465"
     CDS             complement(2519..2797)
                     /gene="catC"
                     /locus_tag="VM406_05465"
                     /EC_number="5.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016159 - muconolactone delta-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muconolactone Delta-isomerase"
                     /protein_id="HVL75444.1"
                     /translation="MLYLVRMDVKLPHDMPASVADDIKQREKAYAQELQRNGKWLHLY
                     RVVGEYANYSVFNVDSNDELHTLLSGLPLFPYMDIKVTPLARHPSAID"
     gene            complement(2816..3976)
                     /locus_tag="VM406_05470"
     CDS             complement(2816..3976)
                     /locus_tag="VM406_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018849 - muconate cycloisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018850 - chloromuconate cycloisomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muconate/chloromuconate family cycloisomerase"
                     /protein_id="HVL75445.1"
                     /translation="MNAPVTIQSVEAILVDLPTIRAHKLAMATMQQQTLVIVRVRCSD
                     GIEGIGEATTIGGLTYGEESPEGTQLTLLTYLAPAMAGMDATNINGVMDHLSRVARGN
                     RLAKSAVETALLDAQGKRLGVPVATLLGGAVRKSLPVLWTLASGDTARDIEEAEALLA
                     KRRHDTFKLKIGRRSIEDDVAHVAGIKRALGARARITVDVNQAWDESIAARAIAMLED
                     VGVDLVEQPIARENRAGMARLAARFVVPIMADEAVATPEDALELARLGAADIFALKIA
                     KSGGLYGMLRTAAVGDAAGIAMYGGTMLEGTIGSIASAHGFCALPRLSWGSELFGPLL
                     VTDDVVVERAVYRDFEMHLPEGPGLGLSLDEDKLAFYRRDRARVLHAVAGGA"
     gene            4081..4983
                     /locus_tag="VM406_05475"
     CDS             4081..4983
                     /locus_tag="VM406_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL75446.1"
                     /translation="MELRHLRYFQAVAKELNFTRAAERLHMAQPPLSRQIRELEEELG
                     VQLFDRSARALKLTEAGRFLNEQSLQLSARLTEIVESTKKIGSNEKQWFGIGFVPSTL
                     YGFIPEMIRAMRGLDAKVEIGLSELITTQQIEALKAGRIDIGFGRIVLDDPAIVRTVL
                     LEEPLIAVLPLDDPLLQQETLTLGQLAARPFILYPARPRPSFADHVMGLCRAHGYALR
                     VVHEANELQTAIGLVASGMGVTLVPASVRGLRRDDIAYRALDTPGFISPVIMSYRADD
                     RSPFLRKVIALVQGVMVDAGGGKE"
     gene            complement(5328..6530)
                     /gene="pcaF"
                     /locus_tag="VM406_05480"
     CDS             complement(5328..6530)
                     /gene="pcaF"
                     /locus_tag="VM406_05480"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HVL75447.1"
                     /translation="MNQAYICDAIRTPIGRYGGALKDVRADDLGAIPLRALMQRNPDV
                     DWAAVQDVIFGCANQAGEDNRNVARMALLLAGLAQEVGGSTVNRLCGSGMDAVGTAAR
                     AIKAGEAGLMIAGGVESMTRAPFVMGKADSAFSRNAALFDTTIGWRFVNPLMKAQYGV
                     DAMPETAENVADDYRIGREDQDRFALASQNKAAAAQQNGVLAQEITPVSIAQKKGEAI
                     VVERDEHPRATSIEALAKLKGVVRPDGTVTAGNASGVNDGACALLLADEAHAARFGLT
                     PRARIVGMATAGVAPRVMGIGPAPAVQKLLAQLGMRLDQIDVIELNEAFAAQGLAVLR
                     QLGLRDDDARVNPNGGAIALGHPLGMSGARLVTTATYQLQRTGGRFALCTMCIGVGQG
                     IAMVIERV"
     gene            complement(6527..7186)
                     /locus_tag="VM406_05485"
     CDS             complement(6527..7186)
                     /locus_tag="VM406_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008350173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HVL75448.1"
                     /translation="MKRLTRDQMAQRVARDIHEGDYVNLGIGLPTLVGNHLPRDREIF
                     LHSENGLLGMGPAPAPGEEDEDLINAGKQPVTLLTGGAYFHHADSFAMMRGGHLDICV
                     LGAFQVSVRGDLANWHTGAPDAIPAVGGAMDLAVGAKRVFVMMEHQTKSGESKIVERC
                     SYPLTGVACVSRIYTDLAVIDVTADGLVVREMVEGLAFEELQRVTGVPLAYQPLTEEM
                     A"
     gene            complement(7183..7869)
                     /locus_tag="VM406_05490"
     CDS             complement(7183..7869)
                     /locus_tag="VM406_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HVL75449.1"
                     /translation="MIDKIYASREAALADIPDGATIMIGGFGNAGMPAALIDALIEQG
                     ARDLTIVNNNAGNGDTGLAALLKAKRVRKIICSFPRQTDSHVFDALYRAGEIELELTP
                     QGNLAERIRAAGAGIGGFFTPTGYGTLLAEGKETRVINGRNYVLESPIHADYALIKAH
                     RADRWGNLVYRKTARNFGPIMAMAAKYTIVQVNEVVELGALDPETIVTPGIFVQRVVQ
                     VSAGAKEERQ"
     gene            8064..8915
                     /locus_tag="VM406_05495"
     CDS             8064..8915
                     /locus_tag="VM406_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HVL75450.1"
                     /translation="MTASKTERQAKAKREGTAAPAPARTVSEEIDALADPDFMTSLAR
                     GLAVIRTFSDQRRSLTIAQISHRTGISRAAVRRCLHTLRQLGYVDADGNSFALKPKVL
                     TLGYSYLSSTPLTVSALPTLNRISRTLSESCSLAVLDDNEVLYVARSATSRIMSVSLN
                     TGSRLPAYCTSLGRTLLAGLDEHALDDYFSQVTLKAFTERTVVAQRRLREILDEVRRD
                     GYALIDEELEVGLRSIAVPVRGASGGVVAALNVGAQAARISARQMRDEFLPVLREGAR
                     ELAVLLP"
     gene            complement(8971..>9914)
                     /locus_tag="VM406_05500"
     CDS             complement(8971..>9914)
                     /locus_tag="VM406_05500"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HVL75451.1"
                     /translation="VHLERIVDDLLDTARMMAGKITLEKKRIDLAEAVGACLRALQAG
                     GRVDGHAIRTALEPVWIDADPARIDQVVNNLLTNALKYTPAGGSIHASVWAQDGCAVF
                     EVADDGLGMAPELLARVFDPFVQGERSLDRAQGGLGIGLTLVRKLVELHGGSVEAASA
                     GPGMGSSFVVRLPLAAADSACTESAAGRPGRTRCVLLVEDNPDVLESTAAVLELADYR
                     VIKARSGTEGLQCALAHAVDVAIVDVGLPDIDGYEVARRLRASSSARGIRLVALTGYG
                     APEDVRRALDAGFDTHLVKPMNLQRLLQALEEAEPAA"
BASE COUNT         1586 a         3333 c         3295 g         1700 t
ORIGIN      
        1 gtcttcaaca tctcgcgcga caagacgctc gcctggctgg acttcgatgg gaccagcctc
       61 ccgaacggca ccctgctcgc ccgggtcgtt gccttcagca gcccgcccgg cggcgcgggc
      121 caggaaatcg aggtgatgcc ggtacggacc tggtatctgc gcaacgaacc acagcccgcg
      181 ctcaacggtc cgatacccgc gccaacgaca atgcccgagg cctggctgcg cctggtcgac
      241 ctgcccttcg tcgatcccga accgcttgcc gaaatgatgc ggctggacga cgccagctac
      301 agccggctga tgaccgacga gccggagcgc gtgcggcaga ccatgtacag gtatgcgccg
      361 gccaacgtgg tgctgtttcc ggcgccgctc gggttcgtgg gcccctggga ttcctgcctc
      421 cagcagcccg cgctgctggc ctgccgggag gcgatgaaca acatgatcgg gctgatgaac
      481 agcaaatgaa gcgcacagcc gcgggcgtgc gggcggccct gctcaggcgg atgcctgcgg
      541 cgcgccttcg ttcaggaagc cgaccactgc gtcggtgaac tgctcacact gctcccagtt
      601 ggacaggtgg gcggcgtcca gctcgacata gcgcgcaccg gcgatgcagc ccgcgacggc
      661 gcggccgtcc gcgggcggcg tggagccgtc atgcgtgccg gcgatcacca gcgtcggcgc
      721 ctggatgcgg ccgagggcgc cgcgctggtc catgtcgcgc acggccgcgc agcaggcggc
      781 gtagccgtcg ggaggcatcg actccagcat cgcgcgcacg cgctcgacct gctgccgcgc
      841 ttcctgctgg aagcgcggcg tgaaccagcg gtcgatgacg gccggggcaa tcgaggccat
      901 gccttcgctc gtcactttcg cgatgcgcgc gttccacagc tcgggcgggc cgatgcgtgc
      961 ggcggtgttg cacagcgcga gacgcccgac gcgttgcgga tgatgaatgc ccagccacat
     1021 gccggtcatg ccgcccatcg acagcccgca gaaatgcgca cgggcgatgt ccaggccatc
     1081 catcaggccg atcacgtcgg cgccgagctg ggcgatcgtg tacgggccgg gcgtgacttc
     1141 cgaacgcccg tgtccgcgcg tgtcgtagcg cagcacccgg aactgccgca acagcgccgg
     1201 catctgcgga tcccacatct cgagcgccgt ccccagcgaa ttcgagagga tgagccaggg
     1261 cgcgccctcg tctccctcga tctggtaatg caagctgccc gactggactg gcatggttgc
     1321 catcaatgcc tccttaacat ccctgacaaa aagccgaagg cagcgttgcg cctccttgcc
     1381 gttctacacg acctggctgc gtcggcgcgc ctagccatcg gcattttgtc agggcttctg
     1441 acttcgtttg cgcgtgcgcc caagcgccgc atggcaggga cggcggactt gccgcccatc
     1501 ccctttaccg catcagttcg cggtgatgtt gcgctccttg atcagcttgg cccagcgatc
     1561 ggtctcgctg cgcaggtagg tgcgcagctc ttccggcgag gagccgaccg gttcggcgcc
     1621 gatcgtcgca aagcgcgcct tcacgtctgg catctgcatc gccttgacga tctcgccatg
     1681 caggcggtcg atcaccggct tgggcgtgtt ggcaggcgcg aacaccgcga accacgggct
     1741 cacctcgtag cccttcaggc cggcttcggc ggcggtcggc acgttcggca gcgtcgcgga
     1801 acgcttcgcg gtggtcaccg ccagcggacg caggcgcccc gattcgatgt gcggcttggc
     1861 cgaggtgatc gagtcgaaca tcatgttgac ctggccgccg atcaggtcgg tgaccgccgg
     1921 gccgctgccc ttgtaaggca cgtgcatgat gtccacgccg gcgatcgagt tgaacatttc
     1981 gccggccatg tggatcgagg tgccgttgcc ggccgacgca taggtcagct tgccgggatt
     2041 ggccttggcc tgggcgacca gttcgctgac cgaattggcc ttgaccgtgg agttgaccac
     2101 gagcacgttg ggcacgatgg cgaccaggct gaccggctcg aaatccttgg ccgcgtcgta
     2161 cggcaggcgc ggatacaggt tgggattgag tgcatgaccg atcgacacga tcatgatcgt
     2221 gtggccgtcc gggtcggcct tcgccaccgt gttggaaccg atggtcccgc ccgcgcccgg
     2281 acggttctcg atgatcaccg gctgcttcag gctctgcgag agcttgtcgc cgagcgtgcg
     2341 cgcgatcacg tccatcgcgc cacccggcgg gaacggcacg acccagcgta ccgtcttggt
     2401 cggccatgcg tcgttcgcat gggcggtgcc ggcgaacgcg gcggtcaatg ccagaccggc
     2461 aagcgcggtc ttcaggaggg cgtgtttcat catgtctcca ttgtagaagg ttggggattc
     2521 aatcgatggc cgacggatgc cgggccagcg gcgtgacctt gatgtccatg tacgggaaca
     2581 gcggcaggcc ggacagcagc gtgtgcagtt cgtcattcga atcgacgttg aagacgctgt
     2641 agttcgcgta ttcgccgacc acgcggtaca ggtgcagcca cttgccgttg cgctggagtt
     2701 cctgtgcgta ggctttctcg cgctgcttga tgtcgtctgc gacggaagcc ggcatgtcat
     2761 gcggcaactt cacgtccatg cgtaccaggt aaagcatcgt catctccggt gttgttcagg
     2821 cgccgcccgc caccgcgtgc agcacgcggg cgcgatcgcg ccggtagaac gcgagcttgt
     2881 cctcgtcgag cgacaggcca aggcccggtc cttcgggcag gtgcatttcg aaatcgcggt
     2941 agaccgcgcg ctcgacgacg acgtcgtcgg tcaccagcag cgggccgaac agctcgctgc
     3001 cccaggacag gcgcggcagc gcgcagaaac catgagcgga cgcgatgctg ccgatcgtgc
     3061 cttcgagcat cgtgccgccg tacatcgcaa tgccggccgc atcgccgacc gcggcggtgc
     3121 gcaacatgcc gtacaggccg ccggacttgg cgatcttcag cgcgaagatg tcggccgcgc
     3181 caaggcgcgc gagctccagc gcatcctcgg gcgtggccac cgcctcgtcg gccatgatgg
     3241 gcacgacgaa gcgtgcggca aggcgcgcca tgccggcacg gttctcgcgg gcgatcggct
     3301 gctcgacgag gtcgacgccg acatcctcca gcatcgcgat cgctcgtgcg gcgatcgatt
     3361 catcccaggc ttggttcacg tcgacggtga tcctggcgcg tgcgccgagc gcgcgcttga
     3421 tgcccgccac atgcgcgaca tcgtcctcga tgctgcggcg gccgatcttc agcttgaagg
     3481 tgtcgtggcg gcgcttggcg agcagcgctt cggcttcctc gatatcgcgg gcggtgtcgc
     3541 cgctggcgag cgtccacagc accggcagtg acttgcgcac ggcgccgccg agcagggtcg
     3601 ccaccggcac gccgaggcgc ttgccctgcg cgtccagcaa ggcggtctcg accgccgact
     3661 tcgccagccg gttgccgcgc gccacgcggg acaggtgatc catcacgcca ttgatgttgg
     3721 tggcgtccat gccggccatc gccggcgcga ggtaggtcag cagcgtcagc tgcgtgcctt
     3781 ccgggctttc ctcgccgtag gtcagcccgc cgatcgtggt cgcctcgccg atgccctcga
     3841 taccgtcgct gcagcgcacg cgcacgatca cgagcgtctg ctgctgcatc gtcgccatcg
     3901 ccagcttgtg ggcgcgaatc gttggcagat cgaccaggat cgcttcgacc gattgaatgg
     3961 tcaccggggc attcatgttg ttacatacct ttaaagtaga gcgggttgaa tagagtatgg
     4021 aacttgacac cacacatgtc caagatcgag tatgtctggt tcgatactca ggaggtatgg
     4081 atggaactcc ggcatctgcg atatttccag gccgtggcca aggagctgaa cttcacccgc
     4141 gcggccgagc gcctgcacat ggcccagccg ccgctgagcc gccagatccg cgaactggag
     4201 gaggagctcg gcgtgcagct gttcgaccgt tcggcgcgcg cgctgaagct gaccgaggcc
     4261 ggacgatttc tcaacgaaca gtcgctgcag ctgagcgcac ggctgacgga aatcgtggag
     4321 agcacgaaga agatcgggtc gaacgagaag cagtggttcg gcatcggctt cgtgccgtcc
     4381 acgctgtatg gcttcatccc ggaaatgatc cgggccatgc gcgggctcga tgcgaaggtg
     4441 gagatcggcc tgtcagagct gatcacgacg cagcagatcg aggcgctgaa ggcggggcgc
     4501 atcgatatcg gattcggccg catcgtgctc gacgatccgg cgatcgtgcg caccgtgctg
     4561 ctggaggaac cgctgatcgc ggtgctgccg ctggacgacc cgctgctgca acaggaaacg
     4621 ctcacgctcg gccagctcgc cgcgcgtcca ttcatcctct acccggcccg cccgcgccca
     4681 agctttgccg accacgtgat gggactgtgc cgggcgcacg gctatgcgct gcgcgtcgtg
     4741 catgaagcaa acgagttgca gaccgcgatc gggctggtcg cctcaggaat gggcgtcacc
     4801 ctcgtcccgg cctcggtgcg cggactgcgg cgcgatgaca tcgcctatcg cgcgctcgat
     4861 acgcccggat tcatctcgcc ggtgatcatg agctatcgcg cggacgaccg ctcgccgttt
     4921 ctgcgcaagg tgattgcgct tgtgcagggc gtgatggtgg acgctggggg cgggaaggaa
     4981 tgaagtgggg gtgaaacgag aacggtgcga cgtcgcattg cactcctgag gggggaaccc
     5041 ggttcggact tcaattgttg tgcaagtggg gagctgcgag cactttgctc ggagtgacag
     5101 caatcggacg atagccccaa cgccgtcgtt ccgagcgaag cgaggaacct catccgcttg
     5161 ctctgcgtct ttgcatgcca gggcgatgag gtccctgacg cgggccgccc tcactgcgtt
     5221 cgggacgaca gtgatcgggt aatggcccta atgctgtcgt tccgagcata gcgaggaacc
     5281 tcatccgctc ggtatgcgcc ggcaggagcc catttcgaac ttcagcccta cacccgctcg
     5341 atcaccatcg cgatcccctg cccgacgccg atgcacatcg tgcacagcgc gaagcgcccg
     5401 ccggtgcgct gcagttgata cgtcgccgtc gtcaccagcc gcgcgccgct catgccgagc
     5461 ggatggccga gtgcgatcgc gccgccgttc ggattcacgc gcgcatcgtc gtcacgcaga
     5521 ccgagctggc gcagcaccgc caggccctgc gcggcaaagg cttcgttgag ctcgatcacg
     5581 tcgatctggt ccagcctcat gccgagctgg gccagcagct tctgcacggc cggggccggg
     5641 ccgatgccca tcacgcgcgg cgcgacaccg gcggttgcca tgccgacgat gcgggcgcgc
     5701 ggcgtcaggc cgaagcgcgc agcatgcgct tcatcggcga gcagcagtgc gcaggcgccg
     5761 tcgttgacac cggacgcatt gcccgccgta acggtgccat cgggacgcac cacgcccttg
     5821 agcttcgcca gtgcttcgat gctggtcgcg cgcgggtgct cgtcgcgctc gacgacgatg
     5881 gcctcgccct tcttctgtgc aatcgacacc ggcgtgattt cctgcgccag cacaccgttc
     5941 tgctgcgcgg cagcggcctt gttctggctg gccagcgcaa atcggtcctg gtcctcgcgg
     6001 ccgatccggt agtcgtcggc gacgttctcc gccgtctccg gcatcgcatc gacgccgtac
     6061 tgcgccttca tcagcggatt cacgaagcgc cagcctatcg tggtgtcgaa cagcgcggca
     6121 ttgcgcgaga acgcgctgtc ggccttgccc atcacaaacg gcgcgcgcgt catcgattcg
     6181 acgccgccgg cgatcatcag ccccgcctcg ccggccttga tcgcacgcgc ggcggtgccg
     6241 accgcatcca tgccggaacc gcacaggcgg ttcacggtgg agccgccgac ttcctgcgcc
     6301 agcccggcca gcagcagcgc catgcgcgcg acgttgcggt tgtcctcgcc ggcctggttc
     6361 gcgcagccga agatcacgtc ctgcacggcc gcccagtcga cgtcggggtt gcgctgcatc
     6421 agcgcgcgca gcggaatcgc gccgagatcg tcggcgcgca catccttcaa tgcgccgccg
     6481 tagcggccga tgggggtgcg gatcgcatcg caaatgtaag cctggttcat gccatttcct
     6541 cggtgagcgg ttggtaggcg agcggcacgc cggtcacgcg ctgcagctcc tcgaatgcga
     6601 gcccttcgac catctcgcgc acgaccagtc cgtcggccgt cacgtcgatg accgcaaggt
     6661 cggtgtagat gcggctcacg caagccacgc cggtcagcgg atagctgcag cgctcgacga
     6721 tcttgctctc gccgctcttg gtctggtgtt ccatcatcac gaacacgcgc ttggcgccga
     6781 cggcgaggtc catcgccccg ccgacggccg gaatcgcgtc cggtgcgccg gtatgccagt
     6841 tcgcgagatc gccgcgaact gacacctgga acgcgccgag cacgcagatg tcgagatggc
     6901 cgccgcgcat cattgcgaac gagtcggcat ggtggaagta ggcgccgccg gtcagcagcg
     6961 tgaccggctg cttgccggca ttgatcaggt cctcgtcttc ctcgccgggc gccggcgccg
     7021 gccccatgcc gagcaggccg ttctcgctgt gcaggaagat ttcgcggtcg cgcggcagat
     7081 ggttgccgac cagggtgggc aggccgatgc cgaggttcac gtaatcgcct tcatggatgt
     7141 cgcgcgccac gcgctgcgcc atctggtcgc gtgtgagtcg tttcattgcc gctcctcctt
     7201 tgctccggcg cttacctgca ccacgcgctg cacgaaaatg ccgggcgtga cgatggtttc
     7261 gggatcgagt gcgcccagct ccacgacctc attcacctgc acgatcgtgt acttggccgc
     7321 catcgccatg atcgggccga agttgcgcgc ggtcttgcga tagaccaggt tgccccagcg
     7381 gtccgcgcga tgcgccttga tcagcgcata gtcggcatgg atcggcgatt ccagcacgta
     7441 gttgcggcca ttgatcacac gggtttcctt gccctcggcc agcagcgtgc catagccggt
     7501 cggcgtgaag aagccgccga tgccggcgcc ggccgcgcgg atgcgctcgg cgaggttgcc
     7561 ctgcggcgtg agttccagtt cgatctcgcc ggcgcggtag agcgcatcga atacatgcga
     7621 gtccgtctgg cgcgggaacg agcagatgat cttgcgcacg cgcttggcct tgagcagcgc
     7681 ggccagtccg gtgtcgccgt tgccggcatt gttgttgacg atggtcaggt cgcgcgcgcc
     7741 ttgctcgatc agcgcatcga tcagcgcagc cggcatgccg gcgttgccga agccgccgat
     7801 catgatggtc gcaccgtcgg gaatgtccgc gagcgcggcc tcgcgcgacg cgtaaatctt
     7861 gtcgatcacg gggtgtcctc ctgggaatcg ggaatgctgc ggggaatact gcggttgact
     7921 ttgtgcggta accgcactac gttgttacgc cagcagcaca gagagtgtat cggcacgaaa
     7981 tcgcaggaaa caagacggca atgatgaagg tgtgcggtaa ccgcacggct tttccagctc
     8041 atacgccggc gggaaagaga cggatgacag cgagcaagac agaaaggcag gcgaaggcaa
     8101 agcgggaagg aacagcggcg cccgcaccgg cgcggacagt ctccgaggag atcgacgcgc
     8161 tcgccgaccc cgatttcatg acctcgctgg cgcgcggcct ggcggtgatc cgcaccttca
     8221 gcgaccagcg ccgcagcctg acaatcgcgc agatcagcca tcgcaccggc atctcgcgcg
     8281 cggcggtgcg gcgctgcctg cacacgctca ggcagctcgg ctacgtggac gcggacggca
     8341 acagcttcgc gctcaagccc aaggtgctta cgctcggcta ctcctacctg tcgtccacgc
     8401 cgctgaccgt ctccgccctg ccgaccctca accggatcag ccgcacgctc agcgaatcct
     8461 gctcgctggc ggtgctcgac gacaacgaag tgctgtatgt cgcgcgctcg gccacctcgc
     8521 gcatcatgtc ggtgtcgctc aacacaggca gccgcctgcc ggcctactgc acctcgctcg
     8581 gtcgaacgct gctcgccggg ctcgacgaac acgcgctcga cgattacttc tcccaagtca
     8641 cgctcaaggc gtttaccgag cgcacggtgg tggcgcaaag gcggctgcgc gagattctcg
     8701 acgaagtgcg gcgcgacggc tacgcgctga tcgacgagga gctggaggtg ggattgcgtt
     8761 cgatcgcggt gccggtgcgc ggggcttcgg gaggcgtcgt ggcagcgctg aacgtggggg
     8821 cgcaggctgc tcgcatcagc gcccggcaga tgcgcgacga gttcctgccg gtgctgcgag
     8881 agggcgcgcg cgagcttgcg gtgctgctcc catgagcggc gcgtccgaga ggcgaggagt
     8941 caggcgcgac ggccttccat ggccagcgcg tcaggccgca ggctcggctt cctccagcgc
     9001 ctgcagcagg cgttgcagat tcatcggctt gaccagatgg gtgtcgaacc cggcgtccag
     9061 cgccctgcgc acgtcctcgg gcgcgccgta tccggtcagc gcgaccaggc ggatcccgcg
     9121 cgcgctagac gacgcgcgca ggcgccgtgc aacctcgtag ccgtcgatgt cgggcagccc
     9181 gacgtcgacg attgccacgt ccacagcatg cgcgagcgcg cattgcagac cttcggtacc
     9241 gctgcgcgcc ttgatcaccc gataatccgc cagctcgagc accgccgccg tcgattcgag
     9301 cacatccgga ttgtcctcga cgagcaggac gcagcgtgtg cgcccgggcc ggccggcagc
     9361 cgattccgtg cacgcggaat ccgccgcagc gaggggcagc cgaaccacga agctgcttcc
     9421 cattcccgga ccggcgctcg ccgcctcgac gctgccgccg tgcagttcca ccagcttgcg
     9481 cacgagcgtg aggccgatcc cgagtccgcc ctgcgcgcgg tccagcgaac gctctccctg
     9541 cacgaagggg tcgaacacgc gcgccagcag ctccggcgcc atgcccaggc cgtcgtcggc
     9601 cacttcgaac accgcgcagc cgtcctgcgc ccacacggac gcatgtatgc tgccgcccgc
     9661 cggcgtgtac ttgagcgcat tggtcagcag gttgttgacc acctggtcga tgcgtgccgg
     9721 gtcggcatcg atccaaaccg gctcgagcgc ggttcggatc gcatgtccgt caacgcgccc
     9781 gcccgcctgc agcgcacgca gacaggcgcc gacggcttcg gccagatcga tacgcttctt
     9841 ttccagcgtg attttcccgg ccatcatccg tgcggtgtcg agcaggtcgt cgacgatgcg
     9901 ctccaggtgg acac
//
LOCUS       DATLGF010000178         2341 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_225380, whole genome shotgun sequence.
ACCESSION   DATLGF010000178 DATLGF010000000
VERSION     DATLGF010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225380"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="VM406_05505"
     CDS             complement(<1..1020)
                     /locus_tag="VM406_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HVL75452.1"
                     /translation="MPAPPSTLPAYAELQCVSNFSFLRGASRPEELVERAATLGYSAL
                     ALTDECSLAGVVRAHVAAREHGLPLLIGSQFRLDSTDGTPPFSLVLLATNRNGYGNLC
                     ELITLGRTRADKGSYLLHPQDLCAPQAELAHLRGMPDCIALLTPEHGIDADLLRRQAQ
                     WLADTFPQRAWLALTLLLRAQDERHRATVMEVAAECTLPVVATGDVCMHVRSRKPLQD
                     TLAAIRLGTPIAECGFALAPNAEQHLRARLRLANIYPPETMNETLRIVERCSFSLDEL
                     RYEYPDELVPPGETPASYLRRETWIGAQRRFPKGVPVKVQELLEHELALIAEMQYEPY
                     FLTVYD"
     gene            1176..>2341
                     /locus_tag="VM406_05510"
     CDS             1176..>2341
                     /locus_tag="VM406_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-dienoyl-CoA reductase FMN-binding
                     domain-containing protein"
                     /protein_id="HVL75453.1"
                     /translation="MTSYPHLLAPLDLGFTTLRNRVVMGSMHTGLEDRFWNYPKLAAY
                     FRERAKGGVGLIVTGGISPNRQGWLLPFGGTMNTPLDVLNHRKVTRAVHEEGGKILMQ
                     ILHAGRYGYQPFVVSASAIKSPISRFAPRALDEAGIERTIRAYVRCATLAQKAGYDGI
                     EIMGSEGYLLNQFLCARTNKRTDRWGGSIENRMRLPVEIVRRVRAAVGHEFIIMYRHS
                     LLDLVEGGNTWEEVVAVARALEAAGVTLLNTGIGWHEARVPTIVTSVPRAAFRSLAGR
                     LKREVSIPVVASNRINMPEQAEAILAEGDADMVSMARPFLADPEFVNKAASGRSDEIN
                     TCIACNQACLDHTFANKRASCLVNPRAGHETELVFRTTARPLRVAVVGAGPAGLS"
BASE COUNT          392 a          755 c          794 g          400 t
ORIGIN      
        1 gtcatagacg gtaaggaagt agggctcgta ctgcatctcg gcgatcagcg cgagttcgtg
       61 ttcgagcagc tcctgcacct tgacgggtac gcctttcggg aaacgccgct gcgcgccgat
      121 ccaggtttcg cggcgcagat agctggcggg cgtctcgccg ggcggcacca gctcatccgg
      181 atattcgtag cgcagctcgt ccagcgagaa gctgcagcgt tcgacgatgc gcagcgtttc
      241 gttcatcgtc tcgggcgggt agatgttggc caggcgcagg cgcgcgcgca ggtgctgctc
      301 tgcattcggc gcgagcgcga aaccgcattc ggcaatcggc gtgccgaggc ggatcgcggc
      361 cagcgtgtcc tgcagcggct tgcgcgagcg cacgtgcatg cagacgtcgc cggtggcgac
      421 caccggcagc gtgcattcgg cggccacctc catcacggtg gcgcggtggc gctcgtcctg
      481 cgcgcgcagc agcagcgtca gcgccagcca tgcgcgctgc gggaaggtgt cggccagcca
      541 ttgcgcctgc ctgcgcagca gatcggcgtc gatgccgtgc tcgggcgtga gtagggcgat
      601 gcagtcgggc atcccgcgca ggtgcgccag ttcggcttgc ggcgcgcaca ggtcctgcgg
      661 atgcagcagg tagctgccct tgtccgcgcg tgtgcggccg agcgtgatca gttcgcacag
      721 gttgccgtag ccgttgcggt tggtcgccaa caaaaccagg gagaacggcg gcgtgccgtc
      781 ggtgctgtcg agccggaact ggctgccgat gagcagcggc aagccatgtt cccttgcggc
      841 cacatgggcg cgcaccacgc cggccagcga gcattcgtca gtcagcgcca gcgcggaata
      901 gccgagcgtg gcggcccgct ccaccagttc ttccggccgc gaggcgccgc gcaggaagct
      961 gaagttggac acgcattgca gttccgcata ggcgggcagg gtggaagggg gagctggcat
     1021 gtttatactg tatgaaaaca cagtataggg aaatcggatg cgcctgtcgt tggcaggaga
     1081 gcaagggtgt tatcagtaca tatagagcct gctcactata aaaacgaacg ctcgttctgc
     1141 tacccttcgt cggttttccc cacacgctgc cggccatgac ttcctacccg catctgctcg
     1201 ctccgctcga tctcggtttc accaccctgc gcaaccgcgt ggtcatgggt tcgatgcaca
     1261 ccggtctcga agaccgcttc tggaactatc cgaagctcgc cgcctacttc cgcgaacgcg
     1321 cgaagggcgg ggtgggcctg atcgtgacag gcggcatttc gccgaaccgg caaggctggc
     1381 tgctgccgtt cggcggcacg atgaacacgc cgctcgacgt gctcaaccat cgaaaagtca
     1441 cccgcgccgt gcacgaggaa ggcggcaaga tcctgatgca gatcctgcat gcgggccgct
     1501 acggttacca gcctttcgtg gtgtccgctt ccgcgatcaa gtcgccgatc tcaagattcg
     1561 cgccgcgcgc gctggatgaa gcggggatcg agcgcacgat ccgcgcctat gtgcgctgcg
     1621 cgacgctggc gcagaaggcc ggctacgacg gcatcgagat catgggcagc gaaggctatc
     1681 tgctgaacca gttcctgtgc gcgcgcacca acaagcgcac cgaccgctgg ggcggcagca
     1741 tcgagaaccg catgcgcctg ccggtggaaa tcgtgcgccg ggtgcgcgcg gcggtcgggc
     1801 acgaattcat catcatgtac cgtcattcgc tgctcgacct ggtcgagggc ggcaatacct
     1861 gggaggaagt ggtggcggtc gcgcgtgccc tggaggcggc cggcgtcacc ctgctcaata
     1921 ccggcatcgg ctggcacgag gcgcgcgtgc cgaccatcgt gacatccgtg ccgcgcgccg
     1981 ccttccgcag cctggcgggg cgcctgaagc gcgaagtttc gatcccggtg gtggcatcca
     2041 accgcatcaa catgccggag caggccgaag ccatcctggc cgagggcgac gccgacatgg
     2101 tgtcgatggc gcgccccttc ctggccgatc ctgaattcgt caacaaggct gcttccgggc
     2161 gcagcgacga gatcaacacc tgcatcgcct gcaaccaggc ctgcctcgat cacacgttcg
     2221 cgaacaagcg cgcgagctgc ctggtcaacc cgcgtgccgg gcatgagacc gaactggtgt
     2281 ttcgcaccac ggcccggccg ctgcgcgtgg ccgtggtcgg cgccggtcct gccggattgt
     2341 c
//
LOCUS       DATLGF010000179         3264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_163693, whole genome shotgun sequence.
ACCESSION   DATLGF010000179 DATLGF010000000
VERSION     DATLGF010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163693"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="VM406_05515"
     CDS             complement(<1..1068)
                     /locus_tag="VM406_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HVL75454.1"
                     /translation="MKTLEVLVVDDEPAIRQILAAYLTKAGCTVSQAQSGGEALERLA
                     RGDVDVAISDIKMPDFTGIELVRRARAAGIDTNFIMMTAHASVDTAIEAIKAGASDYM
                     IKPVRNEEVMHRLTKVRDLRRLSSENRVLRNLVLGSREQSFAFLSPAMKELDRLVAKV
                     APTDSTVLVTGESGTGKGVIARQIHQQSQRADGPFIPVNCGAIPENLMESELFGHVKG
                     SFTGADKARKGLFLEADKGTIFLDEIGELPLALQVKLLHVIEEKTVRPVGGEQARRAD
                     VRIVAATNRDLAQMVALGKFREDLYFRLSVFHVHVPPLRERRQDIPGLIRFLLERGAR
                     RMNSEGGLTLDPDVEQALCAYD"
     gene            1236..>3264
                     /locus_tag="VM406_05520"
     CDS             1236..>3264
                     /locus_tag="VM406_05520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL75455.1"
                     /translation="MNLTSPQLNGILSIADDAIICTDGAQRIILFNQGAERVFGWSAE
                     EIIGKELTELLPERYRASHGAHIERFDKSGEAARKMAERRAIFARHRDGHDFPAEASI
                     SRLGAGDDTVYTVILRDISARKAYERELELAKEKAEAAMQAKSMFLANMSHEIRTPLN
                     AVIGMTSLLLNTRVDDEQRDYAETIRASSEVLLTIINDILDYSKIEIGKLDIEHSPFD
                     LRRCVEESLDLLAPGAAEKDLSLAYLIDDSVPASLVGDVTRLRQVLVNLLSNSIKFTS
                     HGEVVVNVSAQPVGDEYEVCFAVRDTGIGIPEEKQADLFQAFTQVDASTTRKYGGTGL
                     GLAISKRLTEIMGGRMWVESKVGVGSTFYFTLRAPAYGRELAQNFLKERSSSLAGKRV
                     LVVDDNSTNRRLLVKQALLWGMVPFAAASAPEALDMVRHGHAFDVGILDMSMPEMDGL
                     QLALEIRKYRDRQSLPLIMLTSVAQRGRDEHMSAAGFSAYLNKPIKPTALFDALALSM
                     QLTAENRQAPQVIEERLADRLPLKILVAEDNAVNQKVVQQLLAHLGYRADVVANGLEA
                     LDALERQQYDVVLMDIQMPEMDGLEATRRLRSRHGSGGPRVIAMTANAMPGDREKCLA
                     AGMDTYVSKPIELDRIRAVLAEISPVVQRGEPVIDLRRVDQLLELVDDVQCK"
BASE COUNT          608 a         1069 c         1020 g          567 t
ORIGIN      
        1 gtcatacgcg cacagcgcct gttcgacgtc cgggtccaac gtcagcccgc cctcgctgtt
       61 catgcgccgg gccccgcgtt ccaaaaggaa acgtatgagt ccgggaatgt cctggcggcg
      121 ttcgcgcaac ggcggaacat gcacatggaa cacgctcagc cggaaataga ggtcttcccg
      181 gaacttgccc agcgccacca tctgcgcaag gtcgcgattg gtcgcggcga cgatgcgcac
      241 gtccgcgcgc cgggcctgct ccccgcccac cggccgcacc gtcttttcct cgatcacgtg
      301 cagcagcttg acctgcagcg caagcggcag ttcaccgatc tcgtccagaa agatggtgcc
      361 cttgtccgcc tcgagaaaca gtcccttgcg cgccttgtcg gcgccggtga acgagccctt
      421 cacatgaccg aacagctcgc tttccatcag attctcggga atcgcgccgc agttcaccgg
      481 aatgaaggga ccgtcggcac gctggctctg ctggtggatc tggcgcgcaa tcacgccctt
      541 gccggtaccg ctctcaccgg tcaccagcac ggtactgtct gtcggggcga ccttggcgac
      601 gagtcggtca agctccttca tcgccggcga gaggaacgca aagctctgct cgcggctgcc
      661 gagaaccaga ttgcgcagca cccggttttc cgaactgagg cggcgcaggt cgcgcacctt
      721 ggtcagccga tgcatgactt cctcgttgcg caccggcttg atcatgtagt cggacgcgcc
      781 ggccttgatc gcttcgatcg ccgtgtccac cgacgcgtgg gcggtcatca tgatgaagtt
      841 ggtatcgatg cctgcggcgc gggcgcgccg caccagttcg atgccggtga agtccggcat
      901 cttgatgtcg ctgatggcca catcgacatc gccgcgcgcg aggcgttcga gcgcctcgcc
      961 cccgctttgc gcctgcgaca ccgtgcaccc tgccttggtc aagtaagcgg caaggatctg
     1021 gcggatcgcc ggctcgtcgt cgacgacgag aacttcgaga gtcttcattg ttgttgcgga
     1081 catgtcagaa aggcaaattg tggctcagtg gcgcttcact tggaaggata cccccacccc
     1141 ggacggcttg ccacgcgagg gaggggcgct ggccgtctcg ctatacaatg acgcggctcg
     1201 ctgctaaacg ccccaccctg ctccggctcc cgaacatgaa cctgacctcg ccccagctca
     1261 acggcattct cagcatcgcc gacgacgcca tcatctgcac cgacggggcg cagcgcatca
     1321 tcctgttcaa ccagggcgcg gagcgtgtct tcggctggag cgcagaggaa atcatcggca
     1381 aggagctgac ggagctgctg ccggagcgtt accgcgcctc gcacggcgct cacatcgaac
     1441 gcttcgacaa atccggggag gccgcgcgca agatggccga acggcgggca atcttcgcaa
     1501 gacacaggga cggccacgat tttcctgccg aagcctccat ctcgcgcctg ggcgcggggg
     1561 acgacaccgt ctatacggtg atcctgcgcg acatctccgc gcgcaaggcc tacgagcggg
     1621 agctcgaact cgcgaaggaa aaagccgaag cggcgatgca ggcgaagagc atgttcctgg
     1681 ccaacatgag ccacgagatc cgcacgccgc tgaacgcggt catcggcatg accagcctgc
     1741 tgctgaatac ccgcgtcgat gacgagcagc gcgactacgc cgaaaccatc cgcgccagca
     1801 gcgaggtgct gctcacgatc atcaacgaca tcctcgacta ttcgaagatc gagatcggca
     1861 agctcgatat cgagcacagc ccgttcgacc tgcgccgctg cgtcgaggaa tcgcttgacc
     1921 tgctcgcgcc gggtgctgct gaaaaggatt tgagcctggc ctacctgatc gacgatagcg
     1981 tcccggcctc gttggtcggc gatgtcaccc ggctgcgcca ggtgctggtg aacctgctgt
     2041 cgaactcgat caagttcacc tcacacggcg aagtggtggt gaacgtgtcg gcgcagccgg
     2101 tcggcgacga gtatgaagtc tgcttcgccg tgcgcgatac cgggatcggc attccggagg
     2161 agaagcaggc cgacctgttc caggcattca cgcaggtcga cgcctcgacc acccgcaagt
     2221 acgggggtac cggtctcggc cttgccatca gcaagcgcct gactgaaatc atgggcggcc
     2281 gcatgtgggt cgaaagcaag gtcggcgtcg gctccacgtt ttactttacg ctgcgcgcgc
     2341 ccgcttacgg ccgggaactc gcgcagaatt ttctcaagga acgctcatcc tcgctggccg
     2401 gcaagcgggt tctcgtcgtc gacgacaaca gcacgaaccg ccgcctgctg gtgaaacagg
     2461 cgctgctgtg gggcatggtt ccgttcgcgg ccgcctcggc gccggaagcg ctggacatgg
     2521 tgcgccatgg acacgcgttc gatgtcggca tactcgacat gagcatgccg gagatggacg
     2581 gtcttcagct tgcccttgag atccgcaagt accgcgaccg ccagtccctc ccgctgatca
     2641 tgctcacctc ggtggctcag cgggggcgcg acgagcacat gagcgcggcg ggcttttccg
     2701 cctacctgaa caagccgatc aagcccaccg cattgttcga tgcgctcgcc ctttcaatgc
     2761 agctgaccgc cgaaaacagg caggcgccgc aggtgatcga ggaaaggctg gcggatcggt
     2821 tgcccctgaa gattctggtc gccgaagaca atgcggtcaa ccagaaggtt gttcagcaac
     2881 tgctcgccca cctcggctac cgcgccgacg tggtcgccaa cggcctcgaa gcgcttgatg
     2941 cactggagcg ccagcaatac gacgtggtgc tgatggatat ccagatgccc gaaatggatg
     3001 gcctggaagc cacgcgcagg ctgcgttcgc gccacggcag cggcggcccg cgggtgatcg
     3061 cgatgacggc caatgcgatg cccggcgacc gcgagaagtg cctggccgcc ggcatggata
     3121 cctatgtctc gaagccgatc gaactcgaca ggatccgcgc ggtcctggcg gagattagcc
     3181 cggtcgtgca gcgcggcgaa ccggtcatcg acctgcgccg ggtggaccag ctgctcgaac
     3241 tggtcgatga tgtccagtgc aagc
//
LOCUS       DATLGF010000180         5521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_225415, whole genome shotgun sequence.
ACCESSION   DATLGF010000180 DATLGF010000000
VERSION     DATLGF010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225415"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..489)
                     /locus_tag="VM406_05525"
     CDS             complement(28..489)
                     /locus_tag="VM406_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HVL75456.1"
                     /translation="MSSFVRDPKDFWSGWIFVVTGLAVVLIAQDYPMGSAGRMGPAYF
                     PTVLGGLLAVLGAIAVARSMYHRGAGIEPFAFKPLLLIIASVLAFGFLVHGLGLALSI
                     VLLVMISGLGSRKFRPAPFLVLAVAMAAFASLVFIKGLGLPMPLIGSWFGI"
     gene            complement(508..1287)
                     /locus_tag="VM406_05530"
     CDS             complement(508..1287)
                     /locus_tag="VM406_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HVL75457.1"
                     /translation="MLELLADPQVWIAFATLTALELVLGIDNIIFISILVDKLPPERR
                     EFARRIGLLFAMFMRLGLLLILSWIIGMTAPLFTLPILGEEISGRDLVLILGGLFLLW
                     KSVGEIHESLEGAQGQAVRAVKATMAAVIVQVMLVDLVFSLDSIITAIGMVDQIEVMI
                     AAVIASVGLMMLFAGPIGRFVSAHPTVKMLALAFLVVVGVVLIAEGFDHHVPKGYVYS
                     AMAFSLVVEMLNMRLRKPAAEPVHLHSAYEDEEPGRAAEKI"
     gene            complement(1296..1457)
                     /locus_tag="VM406_05535"
     CDS             complement(1296..1457)
                     /locus_tag="VM406_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75458.1"
                     /translation="MYDYVFILALIGASLLWAARKASSDNNRRDSRFMVLMGLISFGA
                     SLSVLALPV"
     gene            1656..3110
                     /locus_tag="VM406_05540"
     CDS             1656..3110
                     /locus_tag="VM406_05540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL75459.1"
                     /translation="MQSRHDARHESLYQAMRRLSRLLADPLRAAITSIMLVILVALLD
                     FATGYEMRLAMLYTVPIALATWVGGARAGALAGAAAVASWGIGFHPTHIYSHPLFYYW
                     EGLMLVMTFAIFILLLHYLRASLARADRRFLHVLDSMPAGIYVTGETSGRLLYANRRF
                     GDLVGEPTDERARRFEQRLHEPSPRPHEDDMPFHNREARDEASGRWYLVQSGSIPWED
                     EERVLLKVVMDITDSKQAEALRQQHHELLHSSARSAVLAEIATTLGHEINQPLMAIAT
                     YLDAGKLLLARQEPALDDVKAALEKARGQVTRASDIVERTRSFLRRRSPTLIHGDINK
                     AAWEAAQALELELQEAGVHIDMELGENLPAVLFDSTLVEQVLINLLRNAAEAMQDSTQ
                     ERRIVLRTARDQDGGLAVSVSDTGPGIPPPVAERLFTPFVTTKPQGLGLGLCICRSII
                     EAHGGALWHEAGEAGGAVFQFTLCAGAAHAKPEA"
     gene            3091..3711
                     /locus_tag="VM406_05545"
     CDS             3091..3711
                     /locus_tag="VM406_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL75460.1"
                     /translation="MPNPKLEPTVFVVDDDEAVRDALKMLLSATGLRVEAHPSAASFL
                     KHYHAAQPGCLVLDIRMPGMSGLALQDELHKRRVSLPIVFLTGHADVPIAVRALKQGA
                     FDFIEKPLDSQRLVIAVMNALRADAEQRARVPVAASEEGKVAGLDTLTEREHEVLGLV
                     LEGKSTRDIAAALSISVKTVEFHRARIREKLGVASLAELFKLFLPR"
     gene            3964..>5521
                     /locus_tag="VM406_05550"
     CDS             3964..>5521
                     /locus_tag="VM406_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HVL75461.1"
                     /translation="MFRDMSIQRKLYAGFGAIVLILLTLLISVYRNSAALTEANARDR
                     NTLQTLVQRNSVEALVLDIQAEFRGFLLTGNEQFLSRLDDNVRALEQHYRRLETLLAD
                     NAAQQERMRRTAALSSEYVQGVVLPLIEKRRALNQAPDASAAMANDPLLSQGFRYVTQ
                     MQGLLRSFDEEENRLHAERERRTTALRDMLFTLLLAGGMVAVLAAVGIALSLARAILS
                     PLSELTSAFTAFGAGNREARARVLQNDEFGRVAQEFNRTAQALQDNEERERRMTDELR
                     AKVDSLLGVVTRAAAGDLTGRVTVGGSDAIGQLGDALARMFDNLRRLIDRVQKAGIQV
                     TTSTTEIAASARQQEGTGVEQAQTSVEVLSTTKEISANSAQLVKTMEEVRHVADYTAT
                     ATTDAQGSLRHMDSTMQRMVSATDGINDKLAALSEKASKINNVLTTIAKVADQTNILS
                     LNAAIEAEKAGEAGRGFSVVATEIRRLADQTSVATWDIEQMLKEMQSAVSASVMGMDK
                     FSDEIRRSATE"
BASE COUNT         1071 a         1808 c         1762 g          880 t
ORIGIN      
        1 ctgctgaaca gttccatcat gctctcctca tatcccgaac cagctgccga tcaggggcat
       61 cggcaggcca agtcccttga tgaagaccag cgatgcaaag gcagccatcg cgaccgcgag
      121 cacaaggaaa ggcgccggcc tgaatttcct gctgcccagc ccgctgatca tcaccagcag
      181 cacgatggat agcgccagcc ccagcccgtg gaccaggaag ccgaaagcca gcaccgacgc
      241 gatgatcagc agcagcggct tgaatgcgaa cggctcgatc ccggcgccgc ggtgatacat
      301 cgagcgcgcg accgcgatcg cgccaagcac cgcaagcagg ccgccgagca cggtcgggaa
      361 gtaggccggc cccatgcggc cggcggagcc catcggataa tcctgggcga tgaggaccac
      421 cgccaggccg gttacaacga agatccaccc ggaccagaag tccttgggat cgcgcacgaa
      481 tgaggacatg ctgtctcctt gttcatttca aatcttctcg gccgctcttc ccggctcttc
      541 gtcttcatag gctgagtgca ggtgcaccgg ctcggcggcc ggcttgcgca gccgcatgtt
      601 cagcatttcc accaccagag agaacgccat cgcgctgtac acatagccct tcggcacgtg
      661 gtgatcgaaa ccttcggcga tcagcaccac gccgaccacc accaggaagg caagcgcgag
      721 cattttcacg gtcgggtgcg cggagacgaa acggccgatc gggccggcaa acagcatcat
      781 caggccgacc gaggcgatga cggcggcgat catgacctcg atctggtcga ccatgccgat
      841 ggcggtgatg atggaatcga gcgagaacac caggtcgacc agcatcacct gcacgatgac
      901 cgcggccatc gtggccttca cggcacgcac ggcctggccc tgcgcgccct ccagcgattc
      961 gtggatctct cccacgctct tccagagcag gaacaggccg ccgagaatga gcaccagatc
     1021 acgtccggaa atctcctcgc cgagaatcgg cagcgtgaac aggggcgcgg tcatgccgat
     1081 gatccaggac aggatcagca ggagtcccag ccgcatgaac attgcgaaga gcaggccgat
     1141 ccggcgcgcg aattcgcgcc gctccggcgg cagcttgtcc accaggatcg agatgaagat
     1201 gatgttgtcg atcccgagga cgagctcgag cgccgtcagc gtcgcaaagg cgatccagac
     1261 ctgcgggtca gcaagaagtt cgagcatggc gatgtctaga ccggaagagc cagcacggac
     1321 aggctggcgc cgaagctgat gaggcccatc aggaccatga agcggctgtc gcggcggttg
     1381 ttgtcggaag acgccttcct ggcggcccac aacagcgagg cgccgatcag ggcgagaatg
     1441 aacacgtagt cgtacaccgt ttcctctttt gaaaagtgat gcgcatccgc tcggcacggg
     1501 acgcgtcggc gggcgcattc aaggtttgcg gtgcagtgta ggcaaaggcg ttcgacgcac
     1561 gactagggtt tcccctagtt ctgcggcgat cgctgcgacg aatcgccgct tgcgtggtgt
     1621 aatgcaagtc ttcctaacgc gagtttttat cctccatgca atcccggcac gatgctcggc
     1681 acgaatccct ctatcaggcg atgcggcggc tgtcgcggct gcttgccgat ccgctgcgcg
     1741 ccgcgatcac ctcgatcatg ctggtgatcc tggtcgcgct gctcgatttt gcgaccggct
     1801 atgagatgcg cctggccatg ctctacacgg tgcccattgc actggcaacc tgggtaggcg
     1861 gcgcgcgcgc cggggcgctt gcgggcgcag cggcggtcgc gagctggggc atcggctttc
     1921 atccgaccca catctactcg catccgctct tctactattg ggaaggcctg atgctggtga
     1981 tgacgttcgc gatcttcatc ctgctgctgc actatctgcg ggcctccctc gcgcgcgcgg
     2041 accggcgctt cctccatgtg ctcgacagca tgccggcagg gatctacgtg accggggaaa
     2101 cgagcggccg gctgctgtat gcgaaccgcc gtttcggcga cctggtcggc gagcccacgg
     2161 acgaacgtgc gcgccggttc gaacaacgct tgcacgagcc gtcaccccgc ccgcatgagg
     2221 acgacatgcc gtttcataac cgggaagcgc gagacgaagc cagcgggcgc tggtacctgg
     2281 tccagtccgg gtccattccg tgggaggacg aggagcgggt gctgctcaag gtggtgatgg
     2341 acatcaccga cagcaagcag gcagaggcgc tgcggcagca gcaccatgaa ctgctgcaca
     2401 gcagcgcgcg ctcggcggtg ctggccgaaa tcgcgaccac gctcggtcac gagatcaacc
     2461 agccgctgat ggcgatcgcc acctacctcg acgccggcaa gctgctgctg gcgcgacagg
     2521 agccggccct cgatgacgtg aaggcggcgc tggaaaaggc ccgggggcag gtcacacggg
     2581 cgagcgacat cgtcgaacgc acgcgcagct tcctgcgtcg ccggtctccg acactgatac
     2641 acggcgacat caacaaggcc gcctgggagg ccgcgcaagc gctggaactg gaactgcagg
     2701 aagcgggtgt tcacatcgac atggagctcg gcgaaaacct gcctgcggta ctgttcgaca
     2761 gcacccttgt cgagcaggtg ctgatcaacc tgctgcgcaa tgccgccgaa gcgatgcagg
     2821 attcaacgca ggaacggcgc atcgtcctgc gcaccgcgcg tgaccaagac ggcgggctcg
     2881 cagtgtcggt cagcgacacc gggccgggca ttccgccgcc ggtggcggag cgcctgttca
     2941 cgccgttcgt caccaccaag ccgcagggtc tgggcctggg gctgtgcatc tgccgctcca
     3001 tcatcgaagc ccacggcggc gccctgtggc atgaggcagg cgaggccggc ggagcggttt
     3061 tccagttcac cctgtgcgcc ggagcggccc atgccaaacc cgaagcttga gccgaccgtt
     3121 ttcgtggtgg acgacgacga ggcggtgcgc gacgcgctga agatgctgtt gagtgcgacc
     3181 ggcctgcgcg tggaggccca tccatcggcc gcctctttcc tcaagcatta ccacgcggcc
     3241 cagcccggtt gcctcgtact cgatatccgc atgccaggca tgagcggcct ggcgcttcag
     3301 gacgaactgc acaagcggcg cgtgagcctg ccgatcgtgt ttctcaccgg ccacgcggat
     3361 gttcccatcg cggtgcgcgc gctcaagcag ggcgcgttcg acttcatcga aaagccgctc
     3421 gactcccaac gcctggtgat cgcagtgatg aatgcgctgc gcgccgacgc cgagcagcgg
     3481 gcgcgcgtgc cggtagcggc aagcgaggag gggaaggtgg cgggtcttga cacgcttacc
     3541 gaacgcgagc acgaggtgct cggcctcgtg ctcgaaggaa aatcgacccg cgatattgcc
     3601 gccgcactca gcatcagcgt gaaaaccgtc gaattccacc gtgcccgcat ccgcgaaaaa
     3661 ctgggcgtgg cctcgctcgc ggaactgttc aagctcttcc tgccgcgctg aaacttctgg
     3721 agcctgcacg tacgtcttcg tgcaaggcat gcttttccgt gcgcaatgaa ccaagcgctc
     3781 caggcgtcgc ctaacgggcg tggagttgcc aaccatcccg gttgcgtatc gtcatgaccg
     3841 catctggtcc aaaaatgtag agttggcgta accccttccg caccgcacgt tgcgagcact
     3901 cgccgcggcc gtcgcccgcc gcccggatgt ccgtgcggca tcgcacccga ctcaggagaa
     3961 tgcatgttca gggacatgag catccagcgc aagctttacg ccggtttcgg tgccatcgtc
     4021 ctcatcctgc tcaccttgct gatcagcgtc taccgcaatt ccgcagcgct caccgaagcc
     4081 aacgcgcgcg accgcaacac cctgcagacc ctggtccagc gcaacagcgt cgaggcgctc
     4141 gtgctcgaca tccaggccga gttccgcgga ttcctgctca ccggaaatga acagttcctt
     4201 tcccggctcg acgacaacgt gcgcgccctc gagcagcatt accggcgtct tgagaccctc
     4261 ctcgccgaca atgctgctca gcaggaacgc atgcggcgca ccgccgcgct cagcagcgag
     4321 tacgtgcagg gcgtggtact gccgctgatt gaaaaacgcc gtgcgctcaa ccaggcgccg
     4381 gatgcaagcg ccgccatggc gaacgatccg ctcctgtcgc agggctttcg ctacgtgacg
     4441 caaatgcagg gactgctgcg ctccttcgac gaggaggaaa accgcctgca cgccgaacgc
     4501 gaacggcgca ccaccgcatt gcgggacatg ctgtttacgc tgctgcttgc cggcggaatg
     4561 gtcgccgtcc tggctgcagt cggcatcgcc ttgtcgctcg cgcgggccat cctgtcgccg
     4621 ctgagcgaac tgaccagcgc cttcaccgca ttcggtgccg gcaaccgcga agcacgggcg
     4681 cgcgtgctgc agaacgatga gttcggccgg gtcgcgcagg agttcaatcg caccgcgcag
     4741 gcgctgcagg acaacgagga gcgcgagcgc cgcatgaccg acgagctgcg cgcgaaggtt
     4801 gattcgctgc ttggcgttgt cacgcgagcg gcagcgggcg acctgaccgg ccgcgtgacg
     4861 gtgggcggca gcgacgccat cggccagctc ggcgacgcgt tggcgcgcat gttcgacaat
     4921 ctgcggcggc tgatagaccg cgtgcagaag gctggcatcc aggtgaccac ctcgaccacc
     4981 gaaatcgccg cctccgcgcg ccagcaggag ggcaccggcg tcgagcaggc gcagaccagc
     5041 gtcgaagtgc tcagcaccac gaaggaaatc tcggccaaca gcgcgcagct ggtcaagacc
     5101 atggaagagg tgcggcacgt ggccgattac acggccacgg ccaccaccga tgcgcagggc
     5161 agcctgcgcc acatggacag cacgatgcag cgcatggtca gcgcgaccga cggcatcaac
     5221 gacaagctcg ccgcgctgtc ggagaaggcg agcaaaatca acaacgtgct gaccacgatc
     5281 gcaaaagtag ccgatcagac caacatcctg tcgctcaatg cggcgatcga ggcggaaaag
     5341 gccggcgaag ccggacgcgg cttctcggtc gtggcaaccg agatccgtcg gctggccgat
     5401 cagacttcgg tggccacctg ggatatcgag cagatgctca aggaaatgca gtccgcggtg
     5461 tcggccagcg tgatgggcat ggacaagttt tccgatgaga tccggcgcag cgccaccgag
     5521 g
//
LOCUS       DATLGF010000181         5870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_54294,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000181 DATLGF010000000
VERSION     DATLGF010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54294"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..484)
                     /gene="rpsO"
                     /locus_tag="VM406_05555"
     CDS             complement(215..484)
                     /gene="rpsO"
                     /locus_tag="VM406_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HVL75462.1"
                     /translation="MSVENINKAAIIAENARGANDTGSPEVQVALLTARINELNGHFK
                     AHAKDHHSRRGLIKMVNRRKSLLAYLKGKDANRYRALIEKLGLRK"
     gene            656..1174
                     /locus_tag="VM406_05560"
     CDS             656..1174
                     /locus_tag="VM406_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75463.1"
                     /translation="MTLLRYAFAFIAVMIAAGCATLFPQPLATGDDEAAIVQKWGRPT
                     AAYQDGNIRILEYRRGPYEQFTYMARVHPTEGLLSAEQVLTNEKFATVQIGKTTRNDI
                     LLTFGRPRETSYLPLKDYEVWTYAYRESGVWNSLMHVHFDRSGVVQMMLSGPDPRFDA
                     DFGRGRSATAAD"
     gene            complement(1158..2696)
                     /locus_tag="VM406_05565"
     CDS             complement(1158..2696)
                     /locus_tag="VM406_05565"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HVL75464.1"
                     /translation="MSDKLIIFDTTLRDGEQSPGASMTKDEKIRIAKQLERLKVDVIE
                     AGFAAASPGDFDAIRAIAGVIKDSTVCSLSRANDRDISRAAEALAPAERRRIHTFIAT
                     SPLHMEKKLRMTPDQVFEQARQAVRFARQFTDDVEFSPEDGSRSDMDFLCRVLESVID
                     EGATTINFADTVGYGVPELYGNMIKTLRERIPNSDKAVWSVHCHNDLGMAVANSLAGV
                     MIGGARQVECTINGLGERAGNTSLEEIVMAVKTRKDYFKLDLGIDTTQIVPASKLVSQ
                     ITGFAVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDVGWTTNKIVLGKLSG
                     RNAFKQRLEELGLELESEAEVNAAFARFKELADRKADIFDEDIIALVADEQNAHENEH
                     YRFVSLSQHSETGEKPQATVVFAIGGKEVRCSGKGNGPVDATVNAIEAEARSGAELVL
                     FSVNAITTGTEAQGEVTMRLSKSGRIVNGVGANPDIVVASAKAYISALNKLFSKSEKL
                     NPQL"
     gene            complement(2982..3860)
                     /gene="pssA"
                     /locus_tag="VM406_05570"
     CDS             complement(2982..3860)
                     /gene="pssA"
                     /locus_tag="VM406_05570"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HVL75465.1"
                     /translation="MATFNRRKAKSSNAGTSRIGRLGKRLSGTPGAAEAANHAVPSLR
                     RRGIYLLPNAFTTANLFAGFFAIVMAMNMRFELAAVAIFGAMLLDTVDGRVARLTNTQ
                     SEFGAQYDSLSDMLSFGAAPALVVYEWSLRGMGKLGWLAAFVYCAGAALRLARFNTNI
                     AVVDKRFFQGLPSPAAAALVTGFVWLMDDLGYSGPDLSWFAWAITLYAGLTMVTNAPF
                     YSFKEINFRKSVPFIAIFLVVLAFVLVSIDPPTVLFGLFVLYGLSGYVMFFWRWLKGK
                     PVSIVQTTENDSDVKD"
     gene            complement(4024..4716)
                     /locus_tag="VM406_05575"
     CDS             complement(4024..4716)
                     /locus_tag="VM406_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HVL75466.1"
                     /translation="MQSSGTLIVVPAYGEVRHPNDEARATFMVEEQDKDRVVAVNRVN
                     QRMKQGTEIIRNEDKTARLETRGYFTFPVYSDEGMQPPQPRAQQQRPLQRQIVGWRVG
                     QYLEVRTTNLEALPRTVAAAQKVLALNGLQFGLADETNRKLEEQRIAAAYENLMERMT
                     AIAKAMGRNVGDMILDTVDFEGSGGYVPQPEVAATRMMSAKAMDAAVVEPGFEPGETT
                     LTMRVVGRARLK"
     gene            complement(5226..>5870)
                     /gene="ilvC"
                     /locus_tag="VM406_05580"
     CDS             complement(5226..>5870)
                     /gene="ilvC"
                     /locus_tag="VM406_05580"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HVL75467.1"
                     /translation="IMVAPKAPGHTVRGTYTQGGGVPHLVAVYQDKSGSARDIALSYA
                     MANGGGRAGIIETNFREETETDLFGEQAVLCGGTVELIKAGFETLVEAGYAPEMAYFE
                     CLHELKLIVDLIYEGGIANMNYSISNNAEYGEYVTGPRIVTEETKNAMRQCLKDIQTG
                     EYAKSFILENKAGAPTLISRRRLTSEHQIEQVGEKLRAMMPWIKKNKLVDQSKN"
BASE COUNT         1106 a         1826 c         1786 g         1152 t
ORIGIN      
        1 ttattgaaca cggtgtttcc tttctctttt ttgccgacag attttcaggc gctgtcgttc
       61 gcctcaccac ggatgacgcc acatcaacac gtcacctctc actgcagaaa tctaaagcct
      121 tgcatgccgc caaatggcaa aaatgcccgc atcagctaac tgacgcaggc atttgcaata
      181 cgcagtgcgg caaagcaatg accggcaacg cgacttactt gcgcagaccg agcttctcga
      241 tgagagcgcg gtagcgattc gcatccttgc ccttcaggta agccagcagg ctcttgcggc
      301 ggttgaccat cttgatcaaa ccgcgacgcg aatggtgatc cttcgcgtgg gccttgaagt
      361 ggccgttgag ttcgttgatg cgagccgtca gcagagcaac ctgcacttcc ggagacccgg
      421 tatcgttggc gccgcgcgcg ttctcggcga tgatagccgc tttgttgatg ttttctacag
      481 acatggggac ctttcacatg cgatgcaagg agtcgcgacc cctagcaacg tgaacgattg
      541 acaataaatc ttggtcaaaa cgaccaagac gcgcagtata gcggaattct ccttgtgctt
      601 caaatacttg gcaccgcgcg acggcgcaag gaccgcgaac cgaggaaccc gtgccatgac
      661 gctactccga tacgcattcg cattcattgc cgtgatgatt gcggccggct gcgcgacgct
      721 gtttccgcag cctctggcga caggcgacga cgaggccgcc atcgtgcaga agtggggccg
      781 ccccaccgct gcctaccagg acggaaacat ccgcatcctg gagtaccgtc gcgggcccta
      841 cgagcagttc acgtatatgg cgcgcgtcca cccgaccgag ggcctgctct cggccgaaca
      901 ggtactgacc aacgaaaaat tcgcgacggt gcagatcggc aagactaccc gcaacgacat
      961 cctgctcact ttcggcaggc cgcgcgaaac ctcctatctg ccgctgaagg attacgaggt
     1021 ctggacctac gcataccgcg aaagcggggt ctggaactcg ctgatgcatg tgcacttcga
     1081 ccggtccggc gtcgtgcaga tgatgctgag cggcccggac ccgcgcttcg acgcggattt
     1141 cggacgaggg cgcagcgcta cagctgcgga ttgagcttct ccgacttcga gaaaagcttg
     1201 ttcagcgccg aaatgtaggc cttcgccgag gccacgacga tgtccggatt ggccccgacg
     1261 ccgttgacga tgcggccgga tttcgacagc cgcatcgtca cctcgccctg ggcttccgtc
     1321 ccggtcgtaa tcgcgttcac cgagaacaga accagctccg cgccgctcct ggcttcggcc
     1381 tcgatcgcat tcaccgtcgc gtcgacgggc ccgttcccct tgccgctgca gcgcacctcc
     1441 ttgccgccga tagcgaacac cacggtcgcc tgcggcttct cgccggtctc cgaatgctgg
     1501 gacagcgaga cgaaacgata atgctcgttc tcgtgcgcat tctgctcatc cgcgaccagc
     1561 gcgatgatgt cctcgtcgaa gatgtcggcc ttgcggtcgg ccagttcctt gaagcgggcg
     1621 aacgccgcat tgacctcggc ctcggactcc agctccaggc caagttcctc gaggcgctgc
     1681 ttgaacgcat tgcggccgga cagcttgcca agcacgattt tgttcgtagt ccagcccaca
     1741 tcttcggcac gcatgatttc gtaggtgtcg cgcgccttga gcacgccgtc ctggtggatg
     1801 ccggaggcat gggcaaacgc attggcgccg accaccgcct tgttgggctg cacggcaaag
     1861 cccgtgatct gggatacgag tttggaagcg gggacgatct gcgtcgtatc gatgcccagg
     1921 tccagcttga agtagtcctt gcgcgtcttg accgccatga cgatctcttc cagcgaggtg
     1981 ttgccggcgc gctcacccag gccgttgatc gtgcactcga cctggcgcgc cccgccgatc
     2041 atcacgccgg ccagtgaatt ggcaaccgcc atcccgaggt cgttgtggca gtgcaccgac
     2101 cagaccgcct tgtccgagtt gggaatgcgc tcgcgcagcg tcttgatcat gttcccgtac
     2161 agctcgggca cgccgtagcc gaccgtgtcg gcgaaattga tcgtggtcgc gccctcgtcg
     2221 atcaccgact ccagcacgcg gcagagaaaa tccatgtccg agcggctgcc gtcttccggg
     2281 ctgaattcca catcgtcggt gaactggcgc gcgaagcgca ccgcctgcct ggcctgttcg
     2341 aagacctggt ccggcgtcat gcgcagcttc ttttccatgt gcagcggcga ggtcgcgatg
     2401 aaggtgtgga tgcggcggcg ctcggccggc gccagcgctt cggcggcacg ggaaatgtcg
     2461 cgatcgttcg cgcgcgaaag cgagcagacg gtggaatcct tgatcacgcc cgcaatcgcc
     2521 cggatcgcgt cgaaatcccc cggagaggcg gcagcaaaac ccgcttcgat cacatccacc
     2581 ttcagtcgct ccagctgctt cgcgatgcgg atcttttcat ccttggtcat cgaagcgccg
     2641 ggcgattgct cgccgtcgcg caaagtcgtg tcgaatatga tcagtttgtc ggacatgatg
     2701 ctgcactcct gagaaaaaac gcgttgacgg aaggtacgga tggttcttcg cctgcccttg
     2761 ccgcaatggc atgagccgga aactgcacga attgtgggta tgggagattt agcgcgcgga
     2821 acgcactagt agcagcggca ctagcaggaa cagcgatagc agcaggccgg agaggctgct
     2881 gccgaaggag aggggtgcgg aaatgtcaat ttcgcggaac atgcgctcga atatacgggc
     2941 aatcctgaaa aagcgcaaca ggccgccgcg acggcgaacc gctagtcctt gacgtcggag
     3001 tcgttctcgg ttgtctgcac gatgctgacc ggcttgccct tcaaccagcg ccagaaaaac
     3061 atcacatagc cggacagtcc atacagcacg aacaggccga acagcaccgt cggcggatca
     3121 atggaaacga gcacgaacgc cagtaccacg agaaagatcg cgatgaatgg cacagacttg
     3181 cggaaattga tttccttgaa gctgtaaaaa ggtgcgttcg tcaccatcgt caggccagcg
     3241 tacagcgtga tcgcccaggc gaaccagctc aggtcgggtc cgctatagcc gagatcatcc
     3301 atcagccata cgaagccggt caccagcgcg gccgctgccg ggctcggcaa gccctggaag
     3361 aagcgtttgt cgaccacggc aatattggta ttgaagcggg ccagtcgcag cgccgccccg
     3421 gcgcaataga cgaaggcggc cagccatccc agcttgccca tgccgcgcag cgaccattca
     3481 tagacgacga gcgcgggcgc cgcgccgaag gaaagcatgt cagacaggct gtcgtactgg
     3541 gcaccgaact cgctctgggt attggtcagg cgcgccacgc gcccgtcgac cgtgtcgagc
     3601 agcatcgcgc caaagatggc gactgcggca agttcgaagc gcatgttcat cgccatcacg
     3661 atggcgaaga aaccggcgaa cagattcgcc gtcgtgaacg cgttcggcaa caggtagatg
     3721 ccgcgccggc gcaggctggg cacggcatgg tttgccgcct cggcggcccc gggcgtgccc
     3781 gagaggcgct tgccgagtcg tccgatcctc gatgtgcctg cgttgctgct cttggccttg
     3841 cggcgattga aggttgccat gcgaattcgt ttcggtgcga tacaagtgag agcagcgttc
     3901 ggcggaattg tttcccaatc cgaacctgcg cgcggccaca gtcgcacgga gctcgatctg
     3961 ttgaagcgtg gctattctag tcgagcaggg atcaaatgta accccttggc aaggccttga
     4021 ggcctacttc aggcgggcgc ggcccacgac gcgcatggtc agcgtggttt ccccgggctc
     4081 gaagcccggc tccacgaccg cggcgtccat cgccttggcg ctcatcatgc gcgtggcggc
     4141 gacttccggc tgcggaacgt atccgccgct gccttcgaag tcgacggtat cgaggatcat
     4201 gtcgccaaca ttgcgcccca tcgccttggc gatcgccgtc atgcgttcca tcaggttctc
     4261 atatgcggcg gcaatgcgct gttcctccag cttccggttc gtttcatcgg ccagcccgaa
     4321 ctgcaggcca ttgagcgcca gcaccttctg ggcggctgcg acggtacgcg gcagggcttc
     4381 cagattggtc gtccgcactt cgagatactg gccgacccgc cagccgacga tctggcgctg
     4441 caacggccgc tgctgctgcg cgcgcggctg cggcggctgc atgccttcgt ccgagtaaac
     4501 cgggaaggtg aagtagccgc gcgtctcgag gcgagcggtc ttgtcttcat tccgaatgat
     4561 ctcggtaccc tgcttcatcc gctgattgac gcggttgact gcgacgacac ggtccttgtc
     4621 ctgttcttcg accatgaagg tggctcgcgc ctcgtcattc ggatgacgaa cctcgccgta
     4681 ggccggcacg acgatcaggg taccgctgct ttgcatcgga gccgcctgct gggctatggc
     4741 ggtagcagaa acgaacaggg cgaaaatgag gggaaggaat cttggcatcg gaatcccggg
     4801 aatggttcgc tcggaagccc ccgtcctgct tcgtgtcaga ttcggccgga ctcgaccgtc
     4861 gaacatgtgg gcgccagggg acaaggcgcc tattttagcc aagcctgcgc gtcagtgatg
     4921 caagacttgc aaacgcttac caggtgcaat ccgacatgcc cgggatgggc ccgatcggag
     4981 gcacttggcg caaaaaaagc cgtgcatgcg cacggccttt cgcttgactg cgggggcact
     5041 tgcaagcgct cccgcgcgtc ggctccgctt gcagcggatc ggttcttcga ctggtcgacc
     5101 agcttgttct ttttgatcca aggcacgtga gcccgaaaaa aagctgtgcc tgcgcacagc
     5161 ttttcgggcg agcgcgggga gcgcttgcaa gcgcttcccg catgatgccc tccgttcgca
     5221 acggctcagt tcttcgattg atcgaccaac ttgttctttt tgatccaagg catcatcgcg
     5281 cgcagcttct cgccgacctg ctcgatctgg tgctcagaag tcagacgacg gcgcgagatc
     5341 agggtcgggg cgccggcctt gttctccagg atgaagctct tcgcgtattc gccggtctgg
     5401 atgtccttca ggcactggcg catcgcgttc ttggtctctt cggtaacgat gcgcggaccg
     5461 gtcacgtact cgccgtattc ggcgttgttc gagatcgagt agttcatgtt ggcgatgccg
     5521 ccttcataaa tcaggtcgac gatcagcttc agttcgtgca ggcactcgaa gtaggccatt
     5581 tccggtgcat aaccggcttc gaccagggtt tcaaaaccgg ccttgatcag ctcgacggta
     5641 ccgccgcaca gcacggcctg ttcgccgaac aggtcggtct cggtctcttc gcggaagttg
     5701 gtctcgatga tgccggcacg gccgccgccg ttggccatcg cgtacgacag cgcgatgtca
     5761 cgggcggagc cggacttgtc ctggtagacg gcgaccaggt gcggcacgcc gccaccctgg
     5821 gtgtaggtgc cgcgcacggt gtggcccggc gccttcggcg cgaccatgat
//
LOCUS       DATLGF010000182         4125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_209904, whole genome shotgun sequence.
ACCESSION   DATLGF010000182 DATLGF010000000
VERSION     DATLGF010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209904"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VM406_05585"
     CDS             <1..236
                     /locus_tag="VM406_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL75468.1"
                     /translation="SSVAREVRQKQLARADGGSAQWEVTMEIRLYRERPSTQRPGKPV
                     VARLWDYLLEREAAADRRVRNATRKRALETEQI"
     gene            411..1697
                     /locus_tag="VM406_05590"
     CDS             411..1697
                     /locus_tag="VM406_05590"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HVL75469.1"
                     /translation="MSPNHELPSYLVPAGLGPWGVYLEQVDRVTPHMGALGRWVETLK
                     RPKRILVVDVPIERDDGSIAHFEGYRVQHNTSRGPGKGGVRFHQDVTLSEVMALSAWM
                     TIKNAAVNVPYGGAKGGIRVDPRTLSDGELQRMTRRYTSEINIIIGPTKDIPAPDVNT
                     NEKVMAWMMDTYSMNQGSTATGVVTGKPISLGGSLGRQEATGRGVFVVGCEAARQRGL
                     SIEGARIAVQGFGNVGGVAARLFAEAGARIVAVQDHVGTVVRESGLDVQALQAHVART
                     GSVAGFPNAESIGERKRFWEVDCDILIPAALEQQITEANADKIRAGIILEGANGPTTP
                     GADDILHDKGVLVVPDVIANAGGVTVSYFEWVQDFSSFFWSEDEINQRLTRIMREAFA
                     AIWQLAQERNVSLRTAAFIIACTRVLQAREMRGLYP"
     gene            1810..3150
                     /locus_tag="VM406_05595"
     CDS             1810..3150
                     /locus_tag="VM406_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HVL75470.1"
                     /translation="MSHVFHRSLRQTYAVAVAGDGPYLIDQSGRRYLDGSGGAAVSCL
                     GHGHPAIVEAVREQVSRLEYAHTSFFTSEPAEALADDLIAHAAAGLSHVYFVSGGSEA
                     VEAALKMAVQYFGETGRPQKNHIIARWQSYHGNTIGALAAGGNRWRRRQFAALLPDMS
                     HIPECYPYRGQRPDESLEQYGQRMADALEAEILRLGPERVAAFIAEPVVGATMGAVTA
                     APGYFQRIREICDRHDVLLILDEVMCGMGRTGTLHACEQEGVRPDLQTIAKGLGAGYQ
                     PIGAVLVNQRIVDAIREGSGFFQHGHTYLGHPVACAAALAVQRVIREEGLLARVRTQG
                     ERLHALLRERLGDHPNVGDLRGRGLFRGIELVADRATRAPLDPAQRVHARIKNAAMSR
                     GLLCYPMGGTIDGEYGDHVLLAPPFTMTDDQLVELADKTAQAICDVLPAAGSPG"
     gene            3209..>4125
                     /locus_tag="VM406_05600"
     CDS             3209..>4125
                     /locus_tag="VM406_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HVL75471.1"
                     /translation="MALAATSLMVAAVPNAAAQQRFMTIGTGGVTGVYYAAGGAICRL
                     VNKERSRHGIRCSVESTGGSVVNINTIRAGELDFGVAQSDWQYHAHKGSKVFERDGAF
                     GDLRAVFSLHPEPFTVVARKEANIQKFEDFKGRRFNVGNPGSGTRASAEELLSAMGWK
                     MSDFSLAAELRPDEHGPALCDNKIDGFLYGVGHPAANIQDPITTCGARLVPLTGPAVD
                     KLVKDAPFYAYAEIPGGLYAGHPNPTRTYGVLATFVTSAKVPEATVYELVRAVFENFD
                     EFKKLHPAFANLDPKKMIQDGNSAPLHPGA"
BASE COUNT          738 a         1333 c         1376 g          678 t
ORIGIN      
        1 aatcgtcggt agcgcgcgag gtgcgccaga agcagctcgc gcgggccgat ggcgggtctg
       61 cccaatggga ggtgacgatg gagattcgtc tctaccgcga gcgaccgagc acccagaggc
      121 cgggcaagcc ggtggtcgca cgcctgtggg actacctgct cgagcgcgag gcggcggccg
      181 acaggcgcgt caggaacgcc acccgcaagc gcgcgctgga gacggaacag atctgagtcg
      241 cgccgccgcc cttccactcc ccatcacccc cttcgcccga tcccgaaaac tatgcattcc
      301 ctgcataggt ggatgcgaac aaagcattgg cgactgcgtg agcgcctgtc ctaggatcct
      361 gcctgcatcg cgctgcatcg tgcagccgta acggacaagg agctttgcgc atgtcgccga
      421 atcacgaact tccttcctat ctcgtgcctg ccggtcttgg gccttggggc gtctacctgg
      481 agcaggtcga ccgggtcacg ccgcacatgg gggcgcttgg acgctgggta gaaaccttga
      541 agcgcccgaa gcgcatcctc gtcgtcgacg ttccgattga acgggacgac ggcagcattg
      601 cccatttcga ggggtaccgg gtgcagcaca acacttcgcg cggccccggc aagggcggtg
      661 tgcgcttcca ccaggacgta acgctctccg aagtgatggc gctttccgcc tggatgacga
      721 tcaagaacgc ggccgtgaat gtgccttacg gtggtgccaa gggtggcatc cgcgtggatc
      781 cgaggacact ctccgacggc gagttgcagc gcatgacgcg ccgttacacc agtgagatca
      841 acatcatcat cggcccgacc aaggacattc ccgccccgga cgtcaatacc aatgaaaagg
      901 tcatggcgtg gatgatggat acgtactcga tgaaccaggg cagcaccgcc accggcgtcg
      961 tgaccggcaa gccgatctcg ctcggcggga gcctgggccg ccaggaggcc accggacgcg
     1021 gcgtgtttgt ggttggctgc gaggcggcca ggcagcgtgg tttgtcgatc gaaggcgcgc
     1081 ggatcgcggt acagggcttc ggcaatgtcg gcggcgttgc cgcccggctg tttgcggaag
     1141 cgggcgccag gattgtcgca gtccaggacc atgtcggcac cgtagtgcgc gagagcgggc
     1201 tcgatgtgca ggcgctgcag gcgcacgtgg cgcggaccgg cagcgtcgca ggattcccga
     1261 acgcggaatc gatcggcgaa cgcaagcgtt tctgggaagt cgattgcgac attctgatcc
     1321 cggcggcgct ggaacagcag atcactgaag ccaacgcgga caagatccgc gccggcatca
     1381 ttctggaagg cgcgaatggc ccgacgacgc cgggcgcgga cgacatcctg cacgacaagg
     1441 gggtgctggt cgtgcccgat gtgatcgcca atgccggcgg cgtcaccgtc agttacttcg
     1501 aatgggtgca ggatttttcc agcttcttct ggagcgagga cgagatcaac cagcgcctga
     1561 cccgcatcat gcgcgaggcc tttgcggcga tctggcagtt ggcgcaggag cggaacgtct
     1621 ccttgcgcac cgcggctttc atcatcgcct gcacacgggt cctgcaggcg cgcgagatgc
     1681 gcgggctgta tccctgatcc gacggcaaga ccgcgggaaa gaatcaagtt ttttcaccgc
     1741 gggttacagc atatgcgttc acttgcggat atcatttcgg cttgatcgaa tgccatcaag
     1801 aagggcaaga tgagtcacgt attccaccgt agcctgcgcc agacctatgc ggtcgcggtt
     1861 gccggtgacg gtccctacct gatcgaccag agcggcaggc gctacctgga tggctccggc
     1921 ggggcggccg tttcctgtct cggtcacggc cacccggcca tcgtggaggc ggtgcgcgaa
     1981 caggtttccc gcctcgaata cgcgcatacc tcgttcttca ccagcgaacc ggccgaagcg
     2041 ctggcggatg acctcattgc ccacgccgcc gcggggctct cccatgtcta tttcgtctcc
     2101 ggcggctccg aagcggtcga ggccgcgctc aagatggcgg tgcagtattt cggtgaaacc
     2161 ggccggccgc agaagaacca catcatcgcg cgctggcaga gctaccacgg caataccatc
     2221 ggcgccctcg ccgcgggcgg caaccgctgg cgcaggcgcc aattcgccgc gctgctgccg
     2281 gacatgagcc acataccgga gtgctacccg tatcgcggtc aacgcccgga tgaaagcctg
     2341 gaacaatatg gccagcgcat ggccgatgcc ctggaagcgg agatcctgcg actggggccg
     2401 gaacgcgttg ccgccttcat cgccgaaccg gtggtgggcg cgacgatggg agcagtgact
     2461 gcggcgccgg ggtatttcca gcgcatccgc gaaatctgcg accgccacga cgtactcctc
     2521 attctcgatg aggtgatgtg cggaatgggg cgcaccggca ccctgcatgc gtgcgagcag
     2581 gaaggcgttc gtccggacct ccagaccatt gccaagggac tgggcgcggg gtatcagccg
     2641 atcggcgcgg tgctggtcaa ccagcgcatc gtcgatgcca tccgcgaagg ctccggcttc
     2701 ttccagcacg gccataccta cctcggccat ccggtcgcct gcgcggcggc gctcgccgtc
     2761 cagcgcgtga tccgggagga gggcttgctg gcgcgcgtac gcacgcaggg cgagcgcctg
     2821 cacgcgctct tgcgcgagcg cctgggtgac catccgaatg tgggcgactt gcgcggccgc
     2881 gggctgtttc gcggcatcga actggtggcc gaccgcgcta cgcgggcgcc gctcgatccg
     2941 gcgcaacgcg tgcatgcccg catcaagaat gctgccatgt ccagggggct gctctgctac
     3001 cccatgggcg gcaccatcga cggcgaatac ggcgaccacg tcctgctcgc tccgccgttc
     3061 accatgaccg atgaccagct tgtggaactg gcggacaaga cggcgcaggc catctgcgac
     3121 gtcctgccgg cagccgggtc cccaggctga acagcggtcc aatatctaca taacggagaa
     3181 agaaaccatg cgcatttcca agaccctgat ggcgctggcg gccacctcgc tgatggtggc
     3241 ggccgtgccg aatgccgcgg cgcagcagcg cttcatgacg atcggcaccg gcggcgtgac
     3301 cggcgtgtat tacgcggccg gcggcgcgat ctgccggctg gtgaacaagg aacgctcgcg
     3361 ccacggcatc cgctgctcgg tggaatcgac cggcggctcg gtggtcaaca tcaacaccat
     3421 ccgggccggc gagctcgatt tcggcgtcgc ccagtccgac tggcaatacc acgcgcacaa
     3481 ggggtccaag gtattcgagc gcgacggcgc gttcggcgac ctgcgcgcgg tgttctcgct
     3541 gcatccggag ccgttcacgg tggtcgcgcg caaggaagcc aacatccaga agttcgaaga
     3601 cttcaagggc cgccgcttca acgtcggcaa ccccggttcg ggcacgcgcg cgtcggccga
     3661 ggaactgctg tcggcgatgg gctggaagat gagcgacttc agcctggccg ccgagctgcg
     3721 tcctgacgag cacggcccgg cgctgtgcga caacaagatc gacggcttcc tgtacggcgt
     3781 cggccatccg gcggccaaca tccaggaccc gatcacgacc tgcggcgcac gcctggtgcc
     3841 gctgaccggt ccggcggtcg acaagctggt caaggatgcg ccgttctacg cgtatgcgga
     3901 aatcccgggc ggcctgtatg ccggccatcc gaacccgacc cgcacctatg gcgtgctggc
     3961 gaccttcgtg acttcggcca aggtgccgga agcgaccgtg tacgaactgg tgcgcgcggt
     4021 gttcgagaac ttcgacgagt tcaagaagct gcacccggcg ttcgcgaacc tggatccgaa
     4081 gaagatgatc caggacggca actccgcgcc gctgcatccg ggcgc
//
LOCUS       DATLGF010000183         3142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_39385,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000183 DATLGF010000000
VERSION     DATLGF010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39385"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..1727)
                     /locus_tag="VM406_05605"
     CDS             complement(222..1727)
                     /locus_tag="VM406_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HVL75472.1"
                     /translation="MNDRNTKLIWAAGALGLVGGAFAANRIASARSRAHPGHRDRAER
                     EMLPKASVLDSLATFMDVFLPNIAKGVIMRRPKVVAMSEHMDLDRRAIRRLQKLRKRY
                     GSGPIMLRIPVRNQAVLVEPAHVQRVLAGSPEPFATASSEKWAALSHFEPKGALISSG
                     TERTVRRRFNEEVLDHERDVHRMVESFMPMVEEEAARIMEAARQHGELSWQEFKEGWF
                     RLVRRVVFGNVAAEDHEFNDMTVELRSAANWAFLRPAKKDLQQRFWARIRHYLDNAAP
                     ESLAGLIARTPHSSEAHPDQQIPQWLFAFDPAGMATFRALALLASHPGAAARAQEEIR
                     TNANRQNLGYLRACVLESLRLWPTTPLILRQTTEETRWENGTMPARTGVTILAPFFHR
                     DDQRLPFADRFTPELWQGERSDRDWPLVPFSGGPAICPARNLVLLLSSAMLASLLEGR
                     EFRLEPPERMDPRKLQSTLDNYSLRFSFREERRAEAPPVQAQAQPEGQTLH"
     gene            complement(1729..2763)
                     /locus_tag="VM406_05610"
     CDS             complement(1729..2763)
                     /locus_tag="VM406_05610"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL75473.1"
                     /translation="MRRKLSDAVVVLTGASSGIGRATAQHFADKGATLVLAARDASAL
                     EETARECRERGATALAVTTDVSREQEVNALAQRALDSFGRIDIWINNAAVCAFGRIDE
                     VPIDVIHRVIETNLMGYVYGTRAALAHMRPRGRGTIIFIDSIVTSSPQPYTGAYVISK
                     AGIRSLSDCLRMELLLEDNPDIDICSIMPESIDTPLFHQAANYTGRAIQTLRPLHSPH
                     TVAREIVRMAERPRRTAFAGTTARWIAALHTMVPWIYERFVARHIYRNLLMDKPAPPT
                     EGNLYHPMPEHTSVTGGWREHHGTSIGKLALAGAALAIPVALWGLANRTRHLPPARGL
                     PHPRRYVEQR"
     gene            complement(2885..>3142)
                     /locus_tag="VM406_05615"
     CDS             complement(2885..>3142)
                     /locus_tag="VM406_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75474.1"
                     /translation="LLGVYQHFYEDNAFGDSGFGTHYVVIACAAELAAGTTLKADAQH
                     ARLQWWDVPALVASSDVHANTKAYFMPARWPAALPADPGRH"
BASE COUNT          487 a          997 c         1106 g          552 t
ORIGIN      
        1 cgtcatggta gcgcggtgag agcgggtctg catcgggtgc cggagattgc gcatttgcat
       61 gcggtggagc tgggttcccg cctgcgcggg aacgacagga cgggggtatc gccaaacgca
      121 agccgaaccc tgcgcttgtg acggcggcca ctcggcgcgt cgctgatttc gcccgagaac
      181 atagcgacaa aactgcgcgt tcacccgcac cgatccccgt ttcaatgcaa ggtctgtccc
      241 tcgggctggg cctgagcctg caccggtggc gcttctgccc gtctctcctc gcggaatgaa
      301 aagcgcagcg agtagttgtc cagcgtcgat tgcagcttcc tcggatccat gcgctcgggc
      361 ggctcgaggc ggaactcgcg tccttcgagc agcgacgcga gcatcgcact ggagagcagc
      421 agcacgaggt tgcgcgccgg gcagatcgca gggccgccgc tgaatggcac caggggccag
      481 tcgcggtcgg agcgttcgcc ctgccagagt tccggcgtga acctgtctgc gaacggcagg
      541 cgctggtcgt cccgatggaa aaatggggcg agtatcgtga cgccggttcg ggccggcatc
      601 gtcccgttct cccagcgcgt ttcctcggtg gtctggcgca ggatcagcgg cgtggtcggc
      661 cacaggcgca gggattcgag cacgcaggcg cgcaggtagc cgaggttctg ccggttcgcg
      721 ttcgtgcgga tttcctcctg tgcgcgcgcg gcggccccgg gatgcgaggc gagcagggcg
      781 agcgcgcgga aggtcgccat gccggccgga tcgaaggcga acagccattg agggatctgc
      841 tgatccgggt gcgcttcgct cgagtgcggc gtgcgcgcga tcagtccggc caggctctcc
      901 ggcgcggcgt tatccaggta gtgcctgata cgcgcccaga accgctgctg caggtccttc
      961 ttcgcggggc gcaggaacgc ccagttggcc gcgctgcgca gttcgaccgt catgtcgttg
     1021 aactcgtgat cctcggcggc cacattgccg aacaccacgc gccgcacgag ccggaaccag
     1081 ccttccttga actcctgcca gctcagctcg ccgtgctgcc gtgcggcttc catgatccgc
     1141 gcggcttctt cctcgaccat cggcatgaaa ctctccacca tccggtgcac gtcgcgctcg
     1201 tgatccagca cctcttcgtt gaagcgccgg cgcacggtgc gttccgtgcc gctgctgatc
     1261 aacgcgccct tgggctcgaa gtgcgacagc gcggcccatt tttcgctgct ggccgtggcg
     1321 aacggctcgg gagagccggc cagcacacgc tggacatggg ccggttccac cagcaccgcc
     1381 tggttgcgca ccggaatacg cagcatgatc ggcccgctgc cgtagcgctt tcgcagcttc
     1441 tgcaggcggc gtatcgcgcg ccggtccaga tccatgtgct cggacatcgc gaccaccttg
     1501 gggcgccgca tgatcacgcc cttggcaatg ttgggcagga agacgtccat gaaggtggcc
     1561 agggagtcga gtacgctcgc cttcggcagc atctcgcgct cggcccggtc cctgtggccg
     1621 ggatgggcgc gggagcgggc ggaggcgatg cggtttgccg cgaaggcgcc gccgaccagg
     1681 ccgagggctc ctgcggccca gatcaatttg gtgtttctgt cgttcatctc agcgttgctc
     1741 cacatagcgg cggggatgcg gcagtccgcg tgccggcggc aggtgccggg tgcggttggc
     1801 cagtccccac agagcgaccg ggatggccag cgccgcgcct gcgagcgcga gtttgccgat
     1861 cgaagtcccg tggtgttcgc gccaccctcc ggtcaccgac gtgtgctcgg gcatcggatg
     1921 gtagaggttg ccctcggtcg gcggggcggg cttgtccatc aacaggttgc ggtagatatg
     1981 acgggcaacg aagcgctcat agatccaagg caccatcgtg tgcagggcgg caatccagcg
     2041 cgccgtcgtg ccggcgaacg cggtgcggcg cggacgctcc gccatgcgta cgatctcgcg
     2101 cgccaccgtg tgcggcgaat gcaatggtcg cagcgtctgg atcgcgcggc cggtgtagtt
     2161 tgcggcctga tggaacagcg gcgtgtcgat cgactccggc atgatgctgc agatgtcgat
     2221 gtccgggttg tcctccagca gcagttccat gcgcaggcag tccgacagcg aacggatgcc
     2281 ggccttgctg atcacatagg ccccggtgta aggctgcggg gaggacgtga cgatggaatc
     2341 gatgaagatg atggtgccgc gcccgcgcgg gcgcatgtgc gccagcgccg cgcgggtgcc
     2401 gtagacgtag cccatcagat tggtttcgat cacgcggtgg atcacatcga tcggcacttc
     2461 gtcgatgcgc ccgaatgcgc acacggcagc gttgttgatc cagatgtcga tgcgaccgaa
     2521 ggagtcgaga gcgcgttgcg ccagcgcatt gacttcctgt tcccgggata cgtcggtcgt
     2581 tacggcgagc gcggtggcac cgcgctcgcg gcattcgcgc gcggtctcct cgagcgccga
     2641 agcatcgcgc gcggcgagta cgagggtcgc ccccttgtcc gcgaaatgct gggcggtcgc
     2701 gcggccgatc ccgctggatg cgccggtcag gacgaccacc gcatcggaca gcttgcgtcg
     2761 cattgtcttc ctccatcggg ccgcggcatg gcggtgcgcg cgcggctggc cgcgccgttg
     2821 caactacggc aagttgtcaa tgaggggtag acgaaggggg aaagggggag ttccgcgaat
     2881 cggatcagtg acggccggga tcggccggaa gggctgccgg ccaccgggcc ggcatgaagt
     2941 aggccttggt gttcgcgtgc acgtcgctgc ttgccaccag tgccggaaca tcccaccact
     3001 gcagacgggc gtgctgggca tcggccttca gcgtcgtgcc ggctgcaagc tcggcagcgc
     3061 aagcgatgac cacgtaatgg gtgccgaaac cgctgtctcc gaacgcgttg tcttcataga
     3121 agtgctggta gacccctaag ag
//
LOCUS       DATLGF010000184         4539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_54360,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000184 DATLGF010000000
VERSION     DATLGF010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54360"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VM406_05620"
     CDS             <1..634
                     /locus_tag="VM406_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL75475.1"
                     /translation="RAVRLRTSVSPSPSPPCERSPDRSQGGLGLGLTLVRSLTALHGG
                     HVEAHSEGPGKGSEFIVHLPRLGEAQEAAMRPAASSAHGDGTLRVMVVDDNADAALSL
                     AMLLESMGYLTTVEYDARRALERAAGEAPDVLLLDIGLPDMDGYTLARRLRAMPATRH
                     ALLIAVTGYGQAEDRERSREAGFDFHLVKPVDTARLAELLGNYGRQARRA"
     gene            complement(648..1439)
                     /locus_tag="VM406_05625"
     CDS             complement(648..1439)
                     /locus_tag="VM406_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HVL75476.1"
                     /translation="MSESDADRLSRFAFAKDRLQILHRLERALEAPMIILGGVWLILM
                     VLELGWSLAPGGARLVNAIWIIFILDFLLRFSLAPRKLAYLRRNWLTAIALVLPAVRT
                     LRMLRMLRLLRGLRLVRIVSSVNRSMKALGKTMRRRGFGYVVALTVIVTLAGAAGMLN
                     FERQVPHEAGLHDFWSALWWTAMMMTTMGSEYWPRTPEGRALGLFLAVYAFAVFGYVT
                     GTIATYFIGRDAEEERSSASKDGIAALQAEIRALREEVRSLRARD"
     gene            complement(1529..3625)
                     /locus_tag="VM406_05630"
     CDS             complement(1529..3625)
                     /locus_tag="VM406_05630"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HVL75477.1"
                     /translation="MSDQTPPTAEVIDEISRARIMDELVDVAAALASAQLDGLTSRIA
                     QALQEREGSCADPALAKLHANAAALLRKNRYPFSYVTVERLRAVLQHELLAASRGLTT
                     SGDTSGALKALPPEIEIDKTLCLRKAGLKIDAAHDERLAPLALRLAHLFGRADLAPAP
                     NPFRAQIFLDVLHDAWCEFHPDTAAHGLLYPLLGELLGLDPAPILHGVNMALVKHGVL
                     PRLQIVPAAAVPAVAADALTAHLRVLLGHAPGQAAMTLLEPHALGRTSLLTYLDSLQH
                     NLFDQHLAACGAEGPKNASVLTHIRRHAPAGSLDQADEAVLDLLAAVFGSVLRQAEIA
                     LELRIAIASLQLPMFKAALADHAFFFRPDQPARRSIELLAEVALGWDPEKGHTDPVFV
                     AVRRNVERIRRGVAERSSVFNDAVWDLESFIRRDLTAADQILAAPIADSLREEKQRGA
                     EKAARTEVALRVGKGEVVAFVETFLEDRWVPVLTLAYGEQDAHPDGVKDALKTMDDLV
                     WSVKPKLTAIERRQLLALLPGMVAALNRWLDRVQWTGAERAQFFAELAQSHASLVRAP
                     LETSPQRQVQLALEAAQRAAERRRQRQAQQPPRREPDMFDRAVHRLERGAWIEFAGPG
                     APRRLRLAWVSPMRSMFVFATRERQYAQSLTDEALAAALREERARIIESAGLVRRALC
                     GALGLPANEDEQQSAA"
     gene            3774..>4539
                     /locus_tag="VM406_05635"
     CDS             3774..>4539
                     /locus_tag="VM406_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL75478.1"
                     /translation="MKEQQQMTHREQPPPASALSPGVSRREVWGWAMYDVANSSYTTV
                     VITAVFNAYFVAVVAGDAAWATFAWTASLAVSHLGLILIGPVIGAYADLRARKKAVLL
                     CSTAGCVLTTAALYFAQPGALAFTVAMLVLSSLFFGLGENLIASFLPELGPADSQGKL
                     SGWGWSIGYFGGLAALVACLAYITWASERGHEASQFVPVTMLITAAFFALASVPTFLL
                     LRERGRPQAVPMPRGVVRDTFTRLAQTWMRAAHYVDL"
BASE COUNT          698 a         1569 c         1573 g          699 t
ORIGIN      
        1 cagggccgtc aggctgcgca ccagcgtcag ccccagtccc agccctccct gcgagcgctc
       61 gcccgaccgc tcgcagggag ggctgggact ggggctgacg ctggtgcgca gcctgacggc
      121 cctgcacgga ggacacgtgg aggcgcacag cgaggggccc ggcaagggca gcgaattcat
      181 cgtgcatctg ccgcgcctgg gcgaggcaca ggaggccgcg atgcgccctg cggcaagcag
      241 cgcgcacggc gacggcacgc tgcgggtgat ggtcgtcgac gacaatgccg acgccgcgct
      301 cagcctggcg atgctgctgg aaagcatggg ttatctcacg acggtcgagt acgacgcgcg
      361 gcgcgcgctg gaacgggcgg ccggcgaggc gccggacgtg ctgctgctcg atatcggcct
      421 gcccgacatg gacggctaca cgctggcgcg gcggctgcgg gcgatgccgg ccacgcggca
      481 cgcgctgctg atcgccgtca ccggctacgg ccaggccgaa gaccgcgagc gttcgcgcga
      541 ggccggtttc gacttccacc tcgtcaagcc ggtcgacacc gcccggctgg ccgagctgct
      601 gggcaactac ggccgccagg cgcggcgcgc ctgagccgcg cgccgcttca gtccctggcg
      661 cgcagcgaac gcacctcctc gcgcagggcg cggatctccg cctgcaatgc cgcgatgccg
      721 tccttgctcg cgctgctgcg ttcttcctcc gcgtcacggc cgatgaagta ggtggcgatg
      781 gtgcccgtga catagccgaa caccgcgaat gcatacactg caagaaacag ccccagcgcc
      841 cgcccttcgg gcgtgcgcgg ccagtattcg cttcccatgg tggtcatcat catcgccgtc
      901 caccacagcg cgctccagaa gtcgtgcagc cccgcttcgt gcggtacctg gcgttcgaag
      961 ttcagcatcc cggccgcgcc ggcgagcgtg acgatgaccg tcagcgccac cacatagccg
     1021 aacccgcgtc gccgcatggt cttgccgagc gccttcatgc tgcggttcac cgaactcacg
     1081 atgcgcacca gacgcagccc gcgcagcagg cgcaacatcc gcagcatgcg cagggtgcgc
     1141 actgccggca gcaccaaggc gatcgccgtc agccagttgc ggcgcaggta cgcaagcttg
     1201 cgcggggcga gcgaaaagcg cagcaggaag tcgagaatga agatgatcca gatcgcattg
     1261 accaggcgcg cgccgcccgg cgccagcgac cagccgagtt cgagcaccat caggatcagc
     1321 cagacgccgc ccaggatgat catcggtgcc tccagcgccc gttcgaggcg gtggagaatc
     1381 tgcagacggt cctttgcgaa cgcaaagcga ctcagccggt cggcatcgga ttcggacatg
     1441 gcagggcgga cgcaccggaa cgggaatgtg cgtttggcaa tgccgcaggc gcgacgttcg
     1501 cgggaatgcc tgcctgactt gcggccgctc aggccgccga ctgctgctcg tcctcgttgg
     1561 ccggcaggcc gagcgcgccg cacagcgcgc gccgcaccag tccggccgat tcgatgatgc
     1621 gcgcgcgctc ctcgcgcagc gccgcggcca gcgcctcgtc cgtcaggctt tgcgcgtact
     1681 ggcgttcccg ggtggcgaac acgaacatcg agcgcatcgg gctcacccag gccagcctca
     1741 ggcggcgcgg cgcacccggg ccggcgaact cgatccaggc gccgcgctcg agccggtgca
     1801 cggcccggtc gaacatgtcc ggctcgcgcc gcggcggctg ctgcgcctgc ctctggcgcc
     1861 gccgttcggc ggcgcgctgg gccgcttcaa gcgcgagctg aacctgccgt tgcggcgagg
     1921 tctcgagcgg ggcgcgcacc agcgacgcat gggattgcgc cagttccgcg aagaattgcg
     1981 cgcgttcggc tccggtccat tggacccggt ccagccagcg gttgagcgcg gccaccatgc
     2041 cgggcagcag cgccagcagt tgccgccgtt cgatcgcagt gagcttgggc ttgacgctcc
     2101 acaccaggtc gtccatggtc ttgagcgcgt ccttcacgcc gtccggatgc gcgtcctgct
     2161 cgccgtacgc aagcgtgagc accggcaccc agcggtcctc caggaaggtt tcgacgaagg
     2221 ccaccacctc gcccttgccc acgcgcagcg caacctcggt gcgcgccgcc ttttccgcgc
     2281 cgcgctgctt ttcttcgcgc agcgagtccg caatgggtgc ggcaaggatc tggtcggcgg
     2341 cggtcaggtc gcggcggatg aaggattcca ggtcccagac ggcgtcgttg aacacgctgc
     2401 tgcgttcggc gacgccgcgc cggatgcgtt cgacattgcg cctgaccgcc acgaataccg
     2461 gatcggtatg gcctttctcc ggatcccagc ccagcgccac ctcggccagc agctcgatgc
     2521 tgcggcgcgc cggctggtcc ggccggaaaa agaacgcatg gtcggccagc gcggccttga
     2581 acatcggcag ctggagcgac gcgatcgcta tgcgcagctc cagcgcgatc tcggcctggc
     2641 gcagcacgct gccgaacaca gcggcaagca ggtcgagcac cgcctcgtcc gcctggtcga
     2701 gactgcctgc cggcgcatgg cggcggatgt gggtgagaac cgaggcattc ttcggacctt
     2761 cggcgccgca tgccgccaga tgctggtcga acaggttgtg ctgcagcgag tccagatagg
     2821 tcagcaggct ggtgcgcccg agcgcatgcg gctcgagcag ggtcatggcg gcctggccgg
     2881 gggcatggcc gagcagcacg cgcagatgcg cggtcagcgc gtcggccgca acagcgggca
     2941 cggcagcggc aggcacgatc tgcaggcgcg gcagcacacc gtgcttgacc agcgccatgt
     3001 tcacgccgtg caggatcggc gccggatcga ggccgaggag ttcgcccagc agcgggtaca
     3061 gcaggccgtg tgccgcagtg tccggatgga actcgcacca tgcgtcatgc agcacgtcca
     3121 ggaagatctg ggcgcggaac gggttcggcg cgggggccag atcggcgcgg ccgaacaggt
     3181 gggccagccg cagcgccagc ggggcaagcc gctcgtcgtg ggcggcgtcg atcttcaggc
     3241 cggccttgcg caggcacagc gtcttgtcga tctcgatctc gggcggcagc gccttgagcg
     3301 cgccgctggt gtcgccgcta gtggtcagcc cgcgcgacgc cgcgagcagc tcgtgctgca
     3361 gcaccgcgcg caggcgctcc acggtgacgt aggaaaacgg gtagcggttc ttccgcaaca
     3421 gggccgcggc gttggcatgc agcttggcca gcgccgggtc ggcgcagctg ccttcgcgct
     3481 cctgcagggc ctgggcgatg cggctggtga ggccgtccaa ctgggcgctg gcaagcgcgg
     3541 cggcgacgtc gaccagttcg tccatgatcc gggcacggga gatctcgtcg atcacttctg
     3601 cggtgggcgg ggtctggtct gacataatga ctctaaggca acacgtaatc cggtaccggg
     3661 gccccaggcg ggcaatcatt gttacacaat cttgcctgag agaaacccat actttgtaaa
     3721 tattcgtatc gttcgcgcaa ctacaagcgg tgcgtgccgc attcgaaccg accatgaagg
     3781 agcaacagca gatgacgcac cgagagcagc caccgccagc cagcgcactg tcgcccggcg
     3841 tgagccgtcg cgaggtctgg ggctgggcga tgtacgacgt cgccaattcc agctacacca
     3901 cggtggtgat caccgccgtc ttcaatgcct atttcgttgc ggtcgtggcc ggcgatgcgg
     3961 cctgggccac ctttgcctgg accgcgtcgc tggcggtgtc gcatctcggg ttgatcctga
     4021 tcgggccggt gatcggcgcc tatgccgacc tgcgcgcgcg caagaaggca gtgctgctgt
     4081 gctcgaccgc cggttgcgtg ctgaccaccg cggcgctcta cttcgcccag ccgggggcgc
     4141 tcgccttcac agtcgcgatg ctggtgctct ccagcctgtt cttcggcctc ggcgagaacc
     4201 tgatcgcgtc cttcctcccc gaactcggtc cggccgacag ccagggcaag ctgtccggct
     4261 ggggctggag catcggctac ttcggcgggt tggcggcgct ggttgcctgt ctggcctaca
     4321 tcacctgggc cagcgaacgc ggtcatgaag ccagccagtt cgttccggtg acgatgctga
     4381 tcaccgccgc cttctttgcg ctggccagcg tgccgacctt cctgctgttg cgcgagcgcg
     4441 ggcggccgca ggcggtcccg atgccgcgcg gggtcgtgcg cgacaccttc acgcggctcg
     4501 cccagacctg gatgcgcgcc gcccactatg tggacctgc
//
LOCUS       DATLGF010000185         2241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_147787, whole genome shotgun sequence.
ACCESSION   DATLGF010000185 DATLGF010000000
VERSION     DATLGF010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147787"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..948
                     /locus_tag="VM406_05640"
     CDS             247..948
                     /locus_tag="VM406_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HVL75479.1"
                     /translation="MKTTSRTLRAVAPDNENGSLSRGEEAYQKMREAIQSGALRPGTR
                     LREVELAEWLGSSRTPVREALNRLQTEGLVVHEPRRGMIIAELDHSMVSELYFMREVL
                     EGTAARLAARHASDVEIAMLREIAERDRQLDDPVKLARNNRMFHETLYRAAHNRYLLK
                     TLSLLQESLALLGPTTLALPGRAGTAYEEHNALVGAIERHDADAAEELSRAHIRSAHR
                     ARMKIMFEQLDDEER"
     gene            981..2027
                     /locus_tag="VM406_05645"
     CDS             981..2027
                     /locus_tag="VM406_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HVL75480.1"
                     /translation="MDSVDLQVLKSCADWLRAGRSAMLVTVVRTWGSSPRPEGAMLAI
                     RDDGRVVGSVSGGCIEDDLIARVRSEGVFVARPQIQRYGISADEAHRFGLPCGGTIEL
                     ALEPLSPKSRIETLLQLLARHQLVARRLDLSTGAVELESAAPGTVLRLTETALTTVHG
                     PPWRLLIIGAGQLSAFLAQVALGMDYQVTVCDPREEYREGWDVPGARLVHAMPDDLVT
                     EMRLDARCAVVALTHDPKLDDLALMEALRSDAFYVGAIGSRLNNARRRERLKLFDLSD
                     AQIGRLRGPVGLYIGSRTPAEIAISILAELTAIRNGVSLPPEARVEYGKRIDRQTGSA
                     HVCALQDDAAPERA"
     gene            complement(1997..>2241)
                     /locus_tag="VM406_05650"
     CDS             complement(1997..>2241)
                     /locus_tag="VM406_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75481.1"
                     /translation="FGISDIFIAAAVFGILGPIFFAIDNRHTAWLERQARAEPAVLEA
                     CAEIAAAARSPYAGAVKEQCLIKFDRSHARSGAASS"
BASE COUNT          390 a          752 c          756 g          343 t
ORIGIN      
        1 attcatcacc tcagggtgtc gcacgtcggc agcaacggcc ctccgctgcg cgcgaaccgg
       61 gcctccccac cttctgcatg atctagtgca cattcgtttg cataacgttg tatgcaccag
      121 tgtacaaaca tcggtagcaa atgtatacgt ttgtgcgcaa agggtctaca ctgtcttcca
      181 aggcccgttt ttcgcgatct gaaacggtta caatccgcgc accatccacc tgccactccg
      241 acaagcatga aaacaacaag cagaacgctg cgcgccgtcg caccggacaa cgagaacggc
      301 tccctgtcgc gcggggagga ggcgtaccag aagatgcgcg aggcgatcca gagcggcgcg
      361 ctgcgcccgg gcacgcgcct gcgcgaagtc gagctggccg aatggctggg cagcagccgc
      421 acgccggtgc gtgaagcgct gaaccggctg cagaccgaag gcctggtggt gcacgagccg
      481 cggcgcggca tgatcattgc ggagctcgac cacagcatgg tgtcggagct ctacttcatg
      541 cgcgaggtgc tggaaggcac ggccgcccgc ctggcggcgc ggcatgcgtc ggatgtggag
      601 atcgcgatgc tgcgcgagat cgccgagcgc gaccgccagc tcgacgatcc ggtcaagctg
      661 gcgcgcaaca accggatgtt tcacgagacg ctgtatcgcg ccgcccacaa ccgctacctg
      721 ctcaagaccc tgagcctgct gcaggaatcg ctggcgctgc tcgggccgac gacgctggcg
      781 ctgccgggcc gcgccggcac cgcgtatgaa gagcacaacg ccctggtggg cgcgatcgaa
      841 cgccacgacg ccgacgcggc cgaggaactg agccgcgccc acatccgctc cgctcaccgc
      901 gcgcggatga agatcatgtt cgaacaactg gatgacgaag aacggtgagc gcccgcgccg
      961 cggcggttgc ctgcgaatga atggatagtg tcgacctcca agtactgaag tcctgcgcgg
     1021 actggcttcg ggccgggcgc agcgcaatgc tggtcaccgt ggtgcgcacc tggggctcga
     1081 gcccgcgtcc ggaaggcgcg atgctggcga tccgcgacga cggccgtgtc gtcggttcgg
     1141 tatccggcgg ctgcatcgag gacgacctga tcgcgcgcgt gcgcagcgag ggcgtgttcg
     1201 tcgcccgccc gcagattcag cggtacggca tcagcgccga cgaggcgcat cgcttcggac
     1261 tgccgtgcgg cggcaccatc gagctggcgc tcgaaccgct gtcaccgaag agccggatcg
     1321 aaacgctgct gcaactgctg gcgcgtcacc agctggtcgc gcgccggctc gatctgtcca
     1381 ccggcgccgt cgaactcgaa agcgccgccc ccggcacggt gctccggctg accgagacgg
     1441 cgctcaccac cgtgcacgga ccgccgtggc gcctgttgat catcggcgcc ggccagctct
     1501 cggcatttct cgcccaggtc gcgctgggca tggactacca ggtcacggtc tgcgatccgc
     1561 gcgaggagta ccgcgaggga tgggatgtgc ccggggcgcg cctggtgcat gcgatgccgg
     1621 acgatctggt gacggaaatg cggctggacg cgcgctgcgc ggtggtcgcg ctcacccacg
     1681 atcccaagct cgacgacctc gcattgatgg aggcgctcag gtccgacgcg ttctatgtcg
     1741 gcgcgatcgg ttcgcgcctg aacaacgcga ggcggcgcga acgcctgaag ctgttcgatc
     1801 tcagcgacgc acagatcggc cggttgcgcg gtccggtcgg cctctatatc ggcagccgca
     1861 cgccggccga gatcgcgatc tccatcctcg ccgaactgac cgcgatcagg aacggcgtct
     1921 cactgcctcc cgaagcgcgc gtggaatacg gcaagcgcat cgaccggcaa acgggcagcg
     1981 cgcatgtctg cgcgcttcag gacgacgcgg cgcccgagcg cgcgtgactg cggtcgaact
     2041 tgatcaggca ctgctctttc acggctcccg cgtaagggct gcgcgcggcc gccgcgattt
     2101 cggcgcaggc ctcgagcact gccggctcgg cccgggcctg gcgctccagc caggccgtgt
     2161 ggcggttgtc gatggcgaag aagatcgggc cgagaatgcc gaatacggcc gccgcgatga
     2221 agatgtcact gatgccgaac c
//
LOCUS       DATLGF010000186         6491 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_23876,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000186 DATLGF010000000
VERSION     DATLGF010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6491
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23876"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VM406_05655"
     CDS             <1..608
                     /locus_tag="VM406_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HVL75482.1"
                     /translation="LAADLVNKICSRSINALLDLRNNLNPLAIEKALNVLARANKIEF
                     WGQGTSGIVAADAQHKFFRSGIPTVAYADPHIHAVSAALLTKGDAVVAISQRGNNTTL
                     LHSVQLARKVGADVIALSPSGTPLADAATVLVPIDLSFNIDPYTPISARLAHLVVIDI
                     LAVGLALKRGPEFRKKMQNAQKALQKLDMQFDSFLRHPPKV"
     gene            673..1608
                     /gene="tal"
                     /locus_tag="VM406_05660"
     CDS             673..1608
                     /gene="tal"
                     /locus_tag="VM406_05660"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HVL75483.1"
                     /translation="MNQLEQLKQYTTIVADTGDFQSIKAFAPRDATTNPSLILKAVQK
                     DEYRPLLEKAVRDNRGAATEEVIDRLIVAFGIEILNIIPGRVSTETDARLSFDTKGTI
                     DKGRALIALYEAAGVARERVLIKIASTWEGIRAAEVLEKEGIRCNMTLLFAMPQAVAC
                     AEARVQLISPFVGRIYDWYKKSTGQEYSGADDPGVQSVQRIYNYYRKFGYNTEVMGAS
                     FRNTGQITELAGCDLLTISPELLQKLADSDAPVERKLSPESAHAADTHKLTLDEKSFR
                     FMLNEDAMGTEKLAEGIRLFCADAVKLEKMVEAAR"
     gene            1698..3500
                     /locus_tag="VM406_05665"
     CDS             1698..3500
                     /locus_tag="VM406_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HVL75484.1"
                     /translation="MPTPPVNVRPASVRADQITQLYASAPIGYCGSVIATLGACAILR
                     EHATQSSILIWVATTLLIAFARAGLSWRYARCPDAEKNDERWLRWFQLSAFAGGFGWG
                     VGGAIMFVPDSVQHQTILLLLLMGMVTAGVYSYAVHLPTFISYLAPMAVGALIGQLTV
                     VAPEWRAYMVFSTLLYVTTSILFARLLSRSLTNAIALKHQNAALAEELRKKSVILEQA
                     SLAKSRFLAAASHDLRQPVHALGLFLASLGRMELSAPARRTVGEMEACVAATGDLFAS
                     ILDVSRLDAGILQPQPVPVAIAPFARRIVAEHQVVALPKGLSFRFRMSPALEQAYVMT
                     DSQLLERIVRNLLQNAIRYTDTGGVLLAVRAHGPRIRFDVVDTGPGIPAQRLDDIFDE
                     FVQLETQAQERHQGLGLGLAIVKRLCPLLDCGIDVVSRVGRGSRFRLTIEAAAGPRAG
                     ADGGEIVQPMALAGKLIVVLDDDPAIVRALTGLLEAWGAQVYGASSLDALLAALAPLT
                     RRPDLIVSDLHLNSGPPGAPSDGLHAVAQIRDEFAEDIPAILITGETAPARLRAAYSA
                     GLPVLHKPLQADALARHLATLFSDTPVGRPAAAA"
     gene            complement(3528..4184)
                     /locus_tag="VM406_05670"
     CDS             complement(3528..4184)
                     /locus_tag="VM406_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL75485.1"
                     /translation="MDTEACARSRRLLPQPHEETAVTTVLIVDDHPLVRSALIQQVYS
                     LLPAATVLTAGTVELARRTASLASSIDLVILDLNLPDENGLSLLCHWGRTMPDVPVIV
                     VSGESEDRRIGEARAAGARAFVAKNASEASLRLAIAEVLQLPVAPEAARAATAFDALT
                     ARQQQVLRALVAGKSNLDIANQLSISEPTVKVHLTAIFRRLGVVSRTQAVLAARAAGW
                     "
     gene            4305..6011
                     /locus_tag="VM406_05675"
     CDS             4305..6011
                     /locus_tag="VM406_05675"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HVL75486.1"
                     /translation="MALDMKRITHPALPSHLLDRTHVRAPLAALVAAAALLIAGCGAG
                     DAAVTGDDNESAIIPLSLVSATLENASTAVPRTAIPQLVFTTDLDPGTVNGANVKLTH
                     AHGSAPLSFAVDGKQLTVVPVDGLLPLTRYSLQVESQVGGKRGQKLASGAVLTFTTAD
                     ASWKQSEAIASDMSIAFSPAIAAGPAGSATAFWVEHVAPNSYLWSRRYTPASGWEAQQ
                     KIQTDPVGIPHSVQLAFDADGNGLAVWPQTSTGNDNIWASRYTQAAGWSAAQLIETND
                     AGSAEKPRLVMNPDGVAMAIWMQWDGSRNRVWSNRFTPEGGWEGARTIDEGWGGNAID
                     TAIAMDRSGNALAFWYHYTGQTLELWTARYEQGMGWGTPGPFDVGHDPHPVMLEDGTA
                     MVAWGHYDGSYHVRYRRYTPITGWGAPATITTSGQVLTAPRLAVDASANASLVWTRGQ
                     SSHYRVWSSQITSDGQWTQPDLIDADPATGAYARLRSFTKDASGNALALWSESLGGNS
                     VTWVNRYTAASGWSTAQVISASNEGMAFEATMAMGISGGIFAIWSQYDGTKYAIMSRR
                     FD"
     gene            6157..>6491
                     /locus_tag="VM406_05680"
     CDS             6157..>6491
                     /locus_tag="VM406_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75487.1"
                     /translation="MTRTTFATSLTSPTCSIIASRHPVNALMLSTCLLLSGCGADNAG
                     TGQVDSNNSILPFSMESISLEDESTGVVRAVAPRFAFTNEPDATAIAANVQLTHAHGS
                     AALSFGVDKT"
BASE COUNT         1183 a         2210 c         1996 g         1102 t
ORIGIN      
        1 acctcgccgc cgacctggtc aacaagatct gcagccgctc gatcaatgcg ctgctcgacc
       61 tgcgcaacaa cctcaatccg ctcgcaatcg aaaaggcgct caatgtgctc gcgcgcgcga
      121 acaagatcga attctggggc cagggcacct ccggcatcgt cgcagccgac gcccagcaca
      181 agttcttccg ttccggcatt ccgaccgtcg cttatgcgga cccgcacatc catgcggtgt
      241 cggcggcgct gctgaccaag ggcgatgcgg tggtggcgat ctcccagcgc ggcaacaaca
      301 ccacgctgtt gcacagcgtg caactggcgc gcaaggtcgg cgccgatgtg attgcgctgt
      361 ccccgagcgg caccccgctt gccgatgcgg caacggtgct cgtgccgatc gacctgagct
      421 tcaacatcga cccctacacg ccgatctcgg cgcggcttgc gcacctggtc gtgatcgaca
      481 tcctcgcggt cggcctggcg ctcaaacgcg gacctgagtt ccgcaagaag atgcaaaacg
      541 cacaaaaggc gctgcagaag ctcgacatgc agttcgattc cttcctgcgc catccaccga
      601 aggtctagca aatcaaactg cgtcgtcatc gtccgggtat cattccggtg tttttccacc
      661 gccagccgcg ccatgaacca gctcgaacaa ctgaagcagt acacgaccat cgttgccgac
      721 accggcgatt tccagtcgat caaggccttc gccccgcgcg acgccaccac caatccctcg
      781 ctgatcctga aggccgtcca gaaggatgaa taccgaccgc tgctggagaa ggcggtacgc
      841 gacaaccgcg gcgccgcgac cgaggaagtg atcgaccgcc tgatcgtcgc cttcggcatc
      901 gagatcctta acatcatccc cggccgtgtc tccaccgaaa ccgacgcccg actgtccttc
      961 gacaccaagg gcacgatcga caagggccgc gcactgatcg cgctctatga agccgccggc
     1021 gtcgcgcgcg agcgcgtgct gatcaagatc gcctccacct gggaaggcat tcgcgccgcc
     1081 gaggtgctcg agaaggaagg catacgctgc aacatgacgc tcctgttcgc gatgccgcag
     1141 gcggtcgcct gtgccgaggc gcgcgtgcag ctgatctcac ctttcgtcgg ccgcatctac
     1201 gactggtaca agaagtccac cggccaggaa tacagcggcg ccgacgatcc cggcgtgcag
     1261 tcggtgcagc gcatctacaa ctactaccgc aagttcggct acaacaccga agtgatgggc
     1321 gccagcttcc ggaacaccgg ccagatcacc gaactggccg gatgcgacct gctgacgatc
     1381 agcccggaac tgctgcagaa actggccgac agcgacgccc ccgtcgagcg caagctgagc
     1441 ccggaatcag cgcacgcggc cgatacccac aagctgacgc tggacgagaa gagcttccgc
     1501 ttcatgctca atgaggatgc gatgggcacc gagaagctgg ccgagggcat ccgcctgttc
     1561 tgcgcggacg ccgtgaagct ggagaagatg gtggaagccg cgcgctgagc cgcgacagga
     1621 cagcgcccgc accatcgccc ggtgaccggt cgggcgctgc cgccccggcg cccgtgagtt
     1681 ccggcttgcc ctcctctatg ccaacgcccc ccgtgaacgt gcgtccggct tccgtacgcg
     1741 ccgaccagat cacccaactt tacgccagcg ccccgatcgg ctattgcggc agcgtcattg
     1801 ccacactggg cgcctgcgcc atcctgcggg aacatgcaac gcagtccagc atcctgatct
     1861 gggtggctac gaccttgctg attgccttcg cacgggctgg cctgtcctgg cgctatgccc
     1921 gttgcccgga cgccgagaag aacgacgagc gctggctgcg ctggtttcag ctctcggcat
     1981 ttgcgggcgg cttcggttgg ggagtaggcg gcgcgatcat gttcgttccg gacagcgtcc
     2041 agcaccagac catcctgctt ctgctgttga tgggcatggt gaccgccggg gtgtactcgt
     2101 atgcggtcca ccttcccact ttcatttcgt acttggcgcc gatggcggtc ggagccctga
     2161 tcggccagct tacggtggtc gcgccggaat ggcgcgccta catggtcttc agcaccttgc
     2221 tttacgtgac gacgtcgatc ctgttcgcac ggttgctctc gcgttcgctg acgaacgcga
     2281 tcgctctcaa gcatcagaac gccgcgctgg ccgaggaact gagaaagaaa tcggtgatcc
     2341 tggagcaggc cagtcttgcc aagtcgcggt ttctcgcggc ggccagccac gacctgcgcc
     2401 agccggtgca cgcactcggc ctgttccttg cgtcgctggg ccgcatggag ctctccgccc
     2461 cggcgcggcg cacggtgggc gagatggaag cctgcgtcgc cgccacgggt gacctgttcg
     2521 cttccatcct tgacgtgtcc cgcctcgacg ccggcatcct tcagcctcaa ccagtgccgg
     2581 tcgcgatcgc gccgttcgcg cggcgtatcg tcgccgaaca ccaggtcgtt gccttgccca
     2641 agggcctgtc attccgcttc cgcatgtcac ccgcgctcga gcaggcgtac gtcatgaccg
     2701 acagccagct cctcgagcgg atcgtgcgca acctgctgca gaacgcgatc cgctacacgg
     2761 acacgggcgg cgtgttgctg gcggtgcgcg cgcacggccc gcgcatccgc ttcgacgtcg
     2821 tcgataccgg ccccggcatt ccggcacagc ggctcgacga tatcttcgac gagtttgttc
     2881 agctggaaac gcaggcgcaa gaacggcatc agggactcgg gctcgggctg gcgatcgtca
     2941 agcggctgtg cccgctgctc gactgcggga tcgatgtcgt ctcgcgcgtc ggccgcgggt
     3001 cgcgctttcg tctcacgatc gaggcggccg caggcccgcg cgccggcgcc gacgggggcg
     3061 agatcgttca gccgatggcc ttggccggaa agcttatcgt cgtgctcgat gacgaccccg
     3121 caatcgtccg cgcattgacc ggcctgctgg aagcctgggg cgcgcaggtc tatggcgcga
     3181 gcagcctgga tgccttgctg gcggcccttg cgccgcttac caggcgtccg gacctgatcg
     3241 tcagcgatct gcacctgaac tccggcccgc ccggcgcgcc gagcgacgga ctgcatgccg
     3301 tcgcgcagat ccgtgacgag ttcgcagagg atatacccgc gattctgatc accggtgaaa
     3361 ccgcccccgc ccggctgcgc gctgcgtact cggccggatt gccggtgctg cacaagccgc
     3421 tgcaagcgga tgcgcttgca cgccacctgg caacgctttt ctccgacacg ccggtcggcc
     3481 gccccgccgc ggccgcatga ccggctggcg cggcggcgac gccgtgctca ccagcccgca
     3541 gcgcgcgccg ccagcaccgc ctgggtccgg ctgaccacgc ccagcctacg gaaaatggcg
     3601 gtcagatgga ccttgaccgt cggctccgag atgctcagct gattggcgat atcaagattg
     3661 ctcttgccgg cgaccagcgc ccgcaacacc tgctgctgcc tggccgtcag cgcatcgaat
     3721 gccgttgcag cacgggcggc ttccggcgcc acgggcaact gcaagacctc ggcaatcgcg
     3781 aggcgcaacg acgcttcgga cgcattcttg gccacgaacg ccctggcgcc ggccgcgcgc
     3841 gcctctccta tgcgccgatc ctcggattca ccgcttacga cgatcaccgg aacatccggc
     3901 atcgtccgcc cccaatggca cagcaatgac aggccgtttt catcgggcag gttcaagtcg
     3961 aggatgacga ggtcaatcga tgaggcgagg cttgcggtgc gccgtgccag ctcgacggtg
     4021 ccggccgtca gcacggtcgc agccggcagc agggagtaca cctgttggat cagcgcactg
     4081 cgcaccaggg gatggtcatc gacgatcagg acggttgtca cggctgtttc ttcgtgcggt
     4141 tgcgggagga ggcggcgact gcgggcacaa gcctcagtat ccatcggcgc cgtgcgcaga
     4201 cgcaacgtct acgacgaaag tcgcattgcc aatgcaacac ttcacgacta gcatgcggct
     4261 cgccgctggc cgctccgata ttgcaggacg agcccggaca ctgcatggcc ctcgatatga
     4321 aacgaatcac acaccccgct ttaccctccc atctcctcga ccgcacccac gtccgcgctc
     4381 cgctggccgc gctcgtcgcc gccgccgccc tcctcattgc gggttgcggc gccggcgatg
     4441 cagccgttac cggggacgac aacgaaagcg cgatcatccc actcagcctg gtaagcgcga
     4501 ccttggagaa tgccagcacc gccgtcccgc gcacggcgat cccgcagctg gttttcacca
     4561 ccgatctcga cccggggacc gtcaacggtg cgaacgtgaa gctgacccac gcgcacggca
     4621 gcgcaccgct ttccttcgca gtggatggca agcagctgac tgtcgtgccg gtagacggac
     4681 tgcttccgct gacccggtat tcacttcagg tcgagtcgca ggtcggcggc aagcgcggcc
     4741 aaaaattggc ttccggggcc gtgctcacgt ttacaaccgc ggatgcgtcc tggaagcagt
     4801 cggaagcgat cgcatccgat atgtcgattg cgttttctcc ggccatcgcg gcggggccag
     4861 cgggcagcgc cactgcattc tgggtcgagc atgtggcgcc aaactcctac ctctggtccc
     4921 gccgctacac ccctgcatcc ggttgggaag cacagcaaaa gattcagacc gatccggtcg
     4981 ggatacctca ttccgttcag cttgcctttg atgccgacgg aaatggactg gcagtctggc
     5041 cacaaaccag caccggcaac gacaacatct gggcgagccg gtacacgcag gccgccggat
     5101 ggtcggcagc acaactgatc gagacgaacg acgcgggcag cgccgagaag ccccgtctgg
     5161 tcatgaaccc tgacggcgtt gcaatggcga tctggatgca gtgggatgga tcccgcaaca
     5221 gggtctggtc caatcggttc acgccggaag gcggctggga aggggcgcgg acgatagacg
     5281 agggctgggg aggcaacgcg atcgacaccg ccatcgccat ggaccgcagc ggcaatgcgc
     5341 ttgcgttctg gtaccactat acgggccaga cattggagct ctggacggcg cgttacgagc
     5401 agggcatggg ctggggaacg ccgggcccct tcgacgtcgg gcacgatccc cacccggtca
     5461 tgttggagga cggcacggcg atggtcgcct ggggccacta tgacggaagc taccatgtgc
     5521 gttacagacg ctacacgccc ataaccggat ggggcgcgcc tgcaacgatt accaccagcg
     5581 gccaagtctt gacggctccg cgccttgcgg tggatgcgag cgccaacgcg agcctcgtgt
     5641 ggacgcgcgg ccagagcagt cactatcgcg tctggtcaag ccagattacg tcagacgggc
     5701 aatggacgca gcccgatctg atcgacgccg atcctgccac cggtgcatat gcccggttac
     5761 ggtcgttcac caaagatgcc agcggaaatg cgctcgcgct gtggagcgaa agcctcggtg
     5821 gaaattcggt gacatgggtg aaccgctata ccgccgcctc cggctggagc accgcgcaag
     5881 tgatcagcgc ctcgaatgaa ggcatggcct tcgaggcgac gatggccatg ggtatcagtg
     5941 gaggcatatt cgcgatctgg tcccagtacg acggaacgaa gtacgcgatc atgtctcggc
     6001 gcttcgactg aagtctgccg gacagaagcc catcccattc tcacgtcgca tgtttgcaac
     6061 acataaaacc aaagtcgcat tggcgcgacg tcaacgtcga cgtagcattc gcccggtcct
     6121 ttgactggac tactcaatct cgccatggaa acgacgatga cgcgtaccac tttcgccacc
     6181 tctctcacct cgccgacgtg ctccatcatt gcttcgcgcc accccgtgaa cgcactgatg
     6241 ctctcaactt gcctgctgct gagcggctgc ggcgccgaca acgccggaac gggacaggtg
     6301 gacagcaaca attccatcct cccgttcagc atggagagca tttcgctgga ggacgagagc
     6361 accggcgtcg tgcgcgcagt cgcacctcgg ttcgcgttca ccaacgagcc ggacgccacg
     6421 gccatcgcgg cgaatgtgca actgacgcat gcccacggca gcgccgccct gtccttcggg
     6481 gtcgacaaga c
//
LOCUS       DATLGF010000187         2147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_134548, whole genome shotgun sequence.
ACCESSION   DATLGF010000187 DATLGF010000000
VERSION     DATLGF010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134548"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1160)
                     /locus_tag="VM406_05685"
     CDS             complement(42..1160)
                     /locus_tag="VM406_05685"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HVL75488.1"
                     /translation="MRIGIPAETRPGESRVAATPETVKKLIAGKHEVLVQSGAGISAS
                     VPDAAFEAAGARLVNAAEAFGCDMVLKVRAPSADERAQMKSGAVVIGMLNPFDADTIA
                     AMAAQGLTAFALEAAPRITRAQSMDVLSSQANIAGYKAVMVAANVYQRFMPMLMTAAG
                     TVKAARVLIMGAGVAGLQAIATAKRLGAVIEASDVRPAAKEQVESLGAKFIDVPFLTD
                     EEREIAQGVGGYARPMPADWMRRQAELVHERAKQADIIITTALIPGRKAPVLIGEETV
                     AAMKPGSVIVDMAVEQGGNCPLSELGRTVVKHGVTIVGEPNLAALVAADASALYARNV
                     LDFLKLVFDKEGQFVINRDDEIVAATLVCSGGEVLRKS"
     gene            1517..2002
                     /locus_tag="VM406_05690"
     CDS             1517..2002
                     /locus_tag="VM406_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HVL75489.1"
                     /translation="MTEIWKPSVTVAAIVEDRGRFLLVEEETSDGIRFNQPAGHLDPN
                     ETLAEAAARETLEEAAYEFFPDSLVGIYMSRYISSRTGLEVTYLRFAFAGALGRALDR
                     PLDEGILRTVWMTREEMLACADRHRSPLVLRCVDDYLAGQRMPLSMLYTHPSSIGAVN
                     V"
     gene            1995..>2147
                     /gene="mnmA"
                     /locus_tag="VM406_05695"
     CDS             1995..>2147
                     /gene="mnmA"
                     /locus_tag="VM406_05695"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325643.1"
                     /note="catalyzes a sulfuration reaction to synthesize
                     2-thiouridine at the U34 position of tRNAs; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HVL75490.1"
                     /translation="MSKGRVVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDS
                     EYCSTRQ"
BASE COUNT          351 a          712 c          667 g          417 t
ORIGIN      
        1 gcgcgcagca tgatgcgttg catgttggga gtttcctatg actaagactt gcgtaagact
       61 tcgccaccgc tgcagaccag cgtggcggcg acgatttcat cgtcgcggtt gatcacgaac
      121 tgcccttcct tgtcgaacac cagcttcagg aagtccagca cgttgcgcgc atacagcgcg
      181 gatgcgtcgg ccgcaaccag cgcggccaga ttcggttcgc cgacgatggt cacgccgtgc
      241 ttgaccaccg tcctgcccag ttcggacagc gggcagttgc cgccctgctc gaccgccatg
      301 tcgacgatca ccgagcccgg cttcatcgcc gcgaccgttt cctcgccgat cagcaccggc
      361 gccttgcggc ccgggatcag cgcggtggtg atgatgatgt cggcctgctt cgcgcgctcg
      421 tgcaccagtt cggcctggcg ccgcatccag tcggccggca tcggccgcgc atagccgccg
      481 acgccctgcg cgatctcgcg ctcctcgtcg gtcaggaacg gcacgtcgat gaacttcgcc
      541 cccagcgact cgacctgttc cttggcggca ggccgcacgt cggaggcctc gatcaccgcg
      601 cccaggcgct tggcggtggc gatcgcctgc aggccggcca cgccggcgcc catgatcagc
      661 acccgcgcgg ccttgacggt gccggccgcg gtcatcagca tcggcatgaa gcgctggtag
      721 acattcgcgg cgaccatcac cgccttgtag ccggcgatgt tggcctgaga cgacagcacg
      781 tccatcgact gggcgcgcgt gatgcgcggc gcggcctcca gcgcgaacgc ggtcaggccc
      841 tgcgccgcca tggcggcgat ggtgtcggca tcgaacggat tgagcatgcc gatgacgacc
      901 gcgccggatt tcatctgcgc ccgctcgtcg gccgacggcg cgcgcacctt cagcaccatg
      961 tcgcagccga aggcttccgc ggcgttcacg aggcgcgcgc cggccgcctc gaaagccgcg
     1021 tccggcacgc tcgcactgat gccggcgccg gactgaacca gcacctcatg ttttccagcg
     1081 atcagcttct tcaccgtctc cggcgtggct gcgactcgtg attctccggg ccgcgtttca
     1141 gcggggatgc cgattctcat tgacctctcc tgttcgtgga acgccagtat tgttcttgta
     1201 acgatcttac atggatttgg aaaattagca cagtcgttct tttttattcc gagtgcgcag
     1261 gctcgcggcg acgattgccg gagcatatcg gaaatcgatc cgtggacacc ggttcacagt
     1321 ttgctttttg ctggggcagc gcaaagcggg acttgcgcgc tctcaatacc cggccgagcc
     1381 aacagcagga tagtcggcgt tcggcgcgcc tcgccattgc ctgttgaaat tcttccgtgg
     1441 ccggtaggtg ttttcctacc ggggtaaaat gtcggccccg tgggagcgcc ctgccgcacg
     1501 agtccctgga attgtcatga ctgaaatctg gaaaccgtcc gtcaccgttg ccgcgatcgt
     1561 cgaggatcgc ggccgcttcc tgctggtcga ggaagaaacg agcgacggca tccgcttcaa
     1621 ccagcccgca gggcatctcg acccgaacga gacgctcgcc gaggcggcag cgcgggaaac
     1681 gctcgaggaa gccgcctacg aattctttcc cgattccctg gtcggcatct atatgtcgcg
     1741 ctacatctcc tcgcgcaccg gactggaagt cacctatctg cgtttcgcct ttgccggcgc
     1801 gctcggtcgc gcgctcgacc gccctctgga cgaaggcatc ctgcgcacgg tctggatgac
     1861 gcgggaggaa atgctggcct gcgccgaccg ccatcgcagt ccgctggtgc tgcgctgcgt
     1921 ggacgactac ctcgccgggc agcgcatgcc gctttccatg ttgtacaccc acccttccag
     1981 cattggcgca gtgaatgtct aaaggacgtg tcgtcattgg aatgtccggg ggcgtggatt
     2041 cctcggtgtc cgcctggttg ctcaaggaac aaggttacga ggtgatcggc ctgttcatga
     2101 aaaactggga ggatgacgac gacagcgagt attgctcgac ccgccag
//
LOCUS       DATLGF010000188        10609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_99880,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000188 DATLGF010000000
VERSION     DATLGF010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 10609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10609
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99880"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1384)
                     /locus_tag="VM406_05700"
     CDS             complement(<1..1384)
                     /locus_tag="VM406_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HVL75491.1"
                     /translation="MNAPYPGVHRLRPLAAAASALFLAGCATFSADGGLNDVSSMTAA
                     RVGQPVQFTKDEKPARPQSVETRQAVSRLLAEPLSPESAVQVALLNNPGLRTSLSELG
                     VAEADLVQAGQMRNPGFTFGRMRDGADVEIERSIGFDVIGLLTIPLRRGIEAQRFEQA
                     KAQAAAEAVRIAAQTRRAWFNAVAAAQTVEYMEQVGSAAEASAELARRMAAVGNWSRL
                     DHAREQVFYAEATAQIARARHHARASREQLARMMGLWGDQLDFRLPNRLPDLPQAPRG
                     IEDFERIAMEQRLDLQMARRDAEATARALGLSRRTGFINVLHAELANVSETGGHRARG
                     YEIELALPIFDWGQARNARAEAIYMGAVHRTADVAVRARSEVREAYSAYRTSYDLARH
                     YRDEVVPLRRLISEEMVLRYNGMLVGVFELLADARVQVAAVNSAIEAQRDYWLAQSDL
                     HMALHGTGGAA"
     gene            complement(1396..1857)
                     /locus_tag="VM406_05705"
     CDS             complement(1396..1857)
                     /locus_tag="VM406_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75492.1"
                     /translation="MSRFLTFLAALVWAPLLMTSLAHAQGAEPAARADAPTAHAPALR
                     YESAFQHYRRFSTETNPPDQVWREANRVVGDLGGHGGHLAEHPSAAKAAGDTTPAMCE
                     KHDTCKDCEGCGECEMCAECEMCEDCDKCGEEDGTNQPDHQPKAHGGHQQH"
     gene            complement(1943..2386)
                     /locus_tag="VM406_05710"
     CDS             complement(1943..2386)
                     /locus_tag="VM406_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75493.1"
                     /translation="MTRRFRTFLLCLLMAALPLQGLAAVMKAACAPEQHALHHGAVKH
                     EQCAECLTAEGSQSVDRSHHHHAASHHGADASPGSSASSGSLSLTDGTCSVCAVCHGA
                     AVAPPVAVLNGLADHVGRLAAPMLAPLLASFIPAGLERPPRYISA"
     gene            complement(2540..3472)
                     /locus_tag="VM406_05715"
     CDS             complement(2540..3472)
                     /locus_tag="VM406_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HVL75494.1"
                     /translation="MKKYLLTLMIAVGSLSMVLPEAEARRMGGGGSFGKQSNNVSRQA
                     TPPAQNAAPAQRQAQPTNAAGAGAAANAAKPSMWKGLLGGALLGLGLGALLSHLGIGG
                     ALASIISTMLMVALFAGVAFFIYRMVTRKNGAPAAKSGFQPAFAGASSTATPEIGSRL
                     QPVQPAAFEAPASASGAAVDANAGTWTMPADFDVAGFVRNAKTYFIRLQAAWDAGNVN
                     DIREFTSPEMFAELRMQLQERGDSANHTDVAQLDAEVLGVETVNDEYLVSVRFTGLIK
                     EEEGADLQSFTEVWNLSKPVNGGGWVLAGIQQVQ"
     gene            complement(3603..3911)
                     /locus_tag="VM406_05720"
     CDS             complement(3603..3911)
                     /locus_tag="VM406_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HVL75495.1"
                     /translation="MKLDKYPRQLLVIIAEAALEKRLIQDVKRFGAHGYTIFDVRGGG
                     TAGVREGIVEADRTIHMEVVCEESVADTIAHHVLTNYAAHYSVTMYFADVQVLRPAKY
                     "
     gene            complement(3920..4870)
                     /locus_tag="VM406_05725"
     CDS             complement(3920..4870)
                     /locus_tag="VM406_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HVL75496.1"
                     /translation="MIDVVVFFFLLGVFARLVKSDLRLPEALYETLSIYLLLAIGLKG
                     GIELSKQPLLALLPQVGVTMLLGLAIPFALYPLFRLLRLNAADSAAIGAHYGSVSVVT
                     FAVVIAKLNDAAIPYEAHAPLWVAVMEMPGLIAGVLLARMFARNQGAGGAPTGWGAIL
                     HDVLIGKSVILLLGGLIIGAVAGVAGTAPIAPVFIDPFKGVLALFLLELGLVAGGRLA
                     EVRRYGLVAFAIGLGVPPVLAVLGALVGVALGLSVGGVAILATLTASASYIAAPTAMR
                     IAVPQANSALSITIALGITFPFNIVFGIPLYIRLAQALTA"
     gene            5012..5908
                     /locus_tag="VM406_05730"
     CDS             5012..5908
                     /locus_tag="VM406_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HVL75497.1"
                     /translation="MARTLWKGAISFGLLHAPIALYTAARSEAVDFDWLHRETLQPIG
                     YKRVIKETGEEVDKEDIVKGVKYGDQYVILSDEEIKSANVESTHTIDIVGFTRAEDVN
                     FLFFEKPYYLAPTKGGEKVYVLLREALLKTGRVGVALVVLHNKQHLAALIATPHAMVL
                     NTLRWASEVSDASELNIPAEGMKAAKIKPKEVEMATQLIESMCDRWDPEQYRDTFRDD
                     IMALVKQKADAGLAQTITEVKAEEPKEEAEEVDLTELLRRSLQGNGKAARTSARKDDG
                     KKAPPPAARKSAGRTRTSKAAH"
     gene            5912..8437
                     /gene="ligD"
                     /locus_tag="VM406_05735"
     CDS             5912..8437
                     /gene="ligD"
                     /locus_tag="VM406_05735"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HVL75498.1"
                     /translation="MAHDLVTYHAKRNFSVTPEPRGELVAPGERLRFVVQKHHARRLH
                     YDFRLEIAGTLKSWAVPKGPSLDPGQKRLAVHVEDHPLDYIDFEGDIPPGQYGAGHVI
                     VWDTGSWEPLGDPQAGYEAGRLRFRLDGEKLHGAWKLVKTGMRGSGDKEQWFLIKEND
                     EEARSTSEYDIVEAEPDSVLGSKGAKAKSRAAPKKKAARPRAREEESAPGLEALPGAR
                     LAPLPGRITPQLATLVDAPPKGGDWLYEIKYDGYRVMACVENGKARLYTRDGHDWSAR
                     MPTQVEAVEALGLDNAWLDGEIVVLDAHGAPSFQLLQNAFDRNGTARIIYYLFDVPYL
                     NGVDLRKTPLQLRRRVLAHLLEHHDSDILRFSAQLDQSPQRLLDRACEMHLEGLIGKR
                     ADSIYVSARSSSWIKLKCRRRQEMIIAGYTEPQGGRQYFGSLVLAVHADDGSLRYAGK
                     VGSGFDDAALADLYRRMQPLEQDACPFDKRPAGLRGVHWVRPKLVAEIAFAEWTGEGV
                     LRQASFQGLRGDKPAAEVRREQATHITARPRARKPADPLLEGVKITNPERVIDAASGA
                     TKLELAHYYREIAPLALPFLQNRPVYLLRAPEGVGGEHFFQKHNTRMTIPGVRKLQIE
                     LISGRAPLMAIETAEALVGAAQMGTIELHIPNVTADRVERPDCMVFDLDPDPDLPWEA
                     MQDAARLTRVVLDELGLTSFLKTSGGKGLHLVVPLSRRHDWDTMHGFAQAVAQHMART
                     LPQRFSAKMGAKNRVGKIFIDYLRNGRSASTIAPYSVRARPGLPVSVPVAWEELDTLK
                     RSGQWTIVDLPELLERAENDPWKAYFETPQNLTAAMRRKLGMK"
     gene            complement(8446..9402)
                     /locus_tag="VM406_05740"
     CDS             complement(8446..9402)
                     /locus_tag="VM406_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HVL75499.1"
                     /translation="MLSIFHTTFPFFALVATGYLAARRGFLPSTAVPALNTFVLYFAL
                     PCMLYRFGASSPIGQLLDASVAAVWLLCALVMVGFTIAMTRSARINWNDASFGALVAA
                     FPNTGFMGLPLLLALIGARAAGPVIITIAIDMVITSSLCIALSRMDDAGANGVRAALK
                     NAIKSIAANPMPWAIVLGVIASAAEFGLPGPIDRTIELLANAASPVALFTIGAVLARS
                     PATGATSLRLSDYVPVALFKLVLHPLLVLLVGAAAIRLGMPLDPFAYMVLILVASLPS
                     ASNVALLAERFGANNERVARIIMVSTVLSFLSFAGVVRLLVG"
     gene            complement(9723..>10609)
                     /locus_tag="VM406_05745"
     CDS             complement(9723..>10609)
                     /locus_tag="VM406_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75500.1"
                     /translation="YARTSLPADFGKNLGNAVRDNPIPIALIGVGLAWLMAQGQRGGG
                     MERGRRYDPYASDSGIEEGYEGGYGLDYSRSDLHGGTQGDRMHDMAERAAEAGRDAKE
                     RVSGASREAMNRMSETGHQMADKAGEMGHRISDAASGMMDRVRHGGEQMRSRMQGSGA
                     GARARMEDLGHRSQEQYYRARDSISHLFEDQPLVLGALGIAIGAALGAALPPTRREDE
                     MLGRTRDDMLDRAKEVGREEAEHVKESARRVAETAKQEGQRVAQDTQARAQGNGHAKE
                     GANPSSPSSGGPGQPSIH"
BASE COUNT         1891 a         3510 c         3585 g         1623 t
ORIGIN      
        1 gcgcggcgcc gccagtacca tgcagcgcca tgtgcagatc gctctgggcg agccagtaat
       61 ctcgctgcgc ctcgatagcg gaattgactg cggcaacctg cacgcgcgca tcggcgagca
      121 gttcgaacac gccgacgagc atgccgttgt agcgcagcac catctcttcc gagatcagcc
      181 ggcgcaaggg caccacttcg tcgcgatagt gcctggcaag gtcataggac gtgcggtagg
      241 cagagtaggc ctcgcgcacc tcggagcgtg cgcgcaccgc cacgtcggcg gtgcggtgta
      301 ccgctcccat gtagatcgcc tcggcacgcg cgttgcgcgc ctgtccccag tcgaagatcg
      361 gcagcgccag ttcgatctca taaccgcgtg cgcggtggcc gcctgtctcg gaaacattcg
      421 ccagttccgc atgcagcaca ttgatgaagc cggtgcggcg cgacaagccc agtgcacgcg
      481 cggtggcctc ggcgtcgcgg cgcgccatct gcaagtccag ccgctgctcc atcgcgatgc
      541 gctcgaagtc ttcgatgccg cgcggcgcct gcggcagatc gggcaggcgg ttcggcagcc
      601 ggaagtccag ctggtcgccc cacaggccca tcatgcgggc aagctgctcg cggctggcgc
      661 gcgcatggtg gcgcgcgcgg gcgatctgcg cggtggcttc ggcgtagaac acctgctcgc
      721 gtgcatgatc gagccggctc cagttgccca ccgccgccat gcgccgcgcc agttccgcgc
      781 tcgcctcggc cgcactgccg acctgttcca tgtactcgac ggtctgcgcg gccgccaccg
      841 cattgaacca ggcgcggcgc gtctgggcgg cgatgcgcac tgcttcggcg gcggcctgcg
      901 ccttggcctg ctcgaaacgc tgcgcctcga tgccgcgtcg cagcggaatg gtgagcaggc
      961 cgatcacatc gaagccgatg ctgcgctcga tctcgacatc ggcgccgtcg cgcatgcgcc
     1021 cgaaggtaaa gccggggttg cgcatttgtc cggcctgcac cagatccgcc tcggccacac
     1081 caagttcgga aagtgaagtg cgcaggcccg gattgttcag gagcgcgacc tgcacggcgc
     1141 tctcgggcga gagcggctcg gccagcaggc gtgacaccgc ctgccttgtc tcgaccgact
     1201 gtggtcgggc cggcttctcg tctttcgtga actggaccgg ctggccgacg cgcgcggccg
     1261 tcatgctgga aacgtcattc aggccgccat cggcggaaaa agtcgcgcag cctgcgagga
     1321 acagcgcgct tgcggcagca gccagcggac gcagccggtg cacgccaggg tatggtgcat
     1381 tcatcgtgtc ctccatcagt gttgctggtg cccgccgtgc gccttgggct ggtggtcagg
     1441 ctggttcgtg ccgtcctcct cgccgcactt gtcgcagtcc tcacacattt cgcattcggc
     1501 gcacatctcg cattcgccgc agccttcgca gtccttgcac gtgtcatgct tttcgcacat
     1561 cgccggcgtg gtatcgccgg ccgccttcgc agcgcttggg tgctcggcca gatggccgcc
     1621 atgaccgccc aggtcgccga ccacgcggtt ggcttcgcgc cagacctggt cggggggatt
     1681 cgtttcggtg gaaaaacgcc ggtagtgttg aaaggccgac tcgtagcgca gcgccggagc
     1741 gtgcgctgtc ggcgcatcgg cgcgagcggc cggctcggcg ccctgtgcat gggcgagcga
     1801 ggtcatgagt agcggcgccc agacgagcgc ggccaggaaa gtcaggaaac gtgacaaaac
     1861 atcctccgtg atgaatcgga acgccctcga tgcgggatcg gggtacgcgg gcgcgaccgg
     1921 cggcaaggcc ggccgtcgac gatcaggcgg agatgtatct tggaggtcgc tcgaggccag
     1981 ccgggatgaa gctggccaac agcggcgcga gcatcggcgc agcaaggcgg ccgacatggt
     2041 ccgccaagcc attcagcacg gccacgggcg gcgccacggc tgcgccgtga cagactgcac
     2101 agacgctgca ggtgccgtct gtgagggaaa gggagccgga cgacgcagaa gagccagggg
     2161 acgcatcggc gccatggtga ctggcggcat ggtggtgatg cgagcggtcg accgattggg
     2221 aaccttcggc cgtcaggcac tcggcacact gttcatgttt caccgcgccg tgatgcaagg
     2281 catgctgctc cggagcacaa gcggccttca tgacggccgc cagcccttgc aggggcagcg
     2341 ctgccatcag gaggcacagc aggaaggtcc ggaatcggcg ggtcatttga gcctgttcgt
     2401 ttgcctgtcg caggcgagta gtttctgaga acgagtcgag agcagtgcga agtctagccc
     2461 ttccaatggg gggaaggtca agagggaata aaagaaaagc cgggtcgccc cggcttttcc
     2521 cctgatgcaa ttacaacgct tactgcacct gctgaatccc cgccagcacc cagccgccgc
     2581 cgttgaccgg cttggacagg ttccagactt cggtgaagga ttgcaggtcc gcgccctctt
     2641 cttccttgat caggccggtg aagcgcacgc tgaccaggta ctcgtcgttg acggtctcca
     2701 cgcccagcac ttcggcgtcg agctgcgcga cgtcggtgtg gttggcggag tcgccgcgct
     2761 cctgcagctg catgcgcagc tcggcgaaca tttccggcga ggtgaattcg cggatgtcgt
     2821 tcacgttgcc ggcgtcccag gcggcctgca ggcggatgaa gtaggtcttg gcattgcgga
     2881 caaaaccggc cacgtcgaag tcggcaggca tggtccaggt gccggcgttg gcgtcgaccg
     2941 cggcgccgga agcggaagcc ggtgcctcga acgcggcagg ctgcacgggt tgcagacgcg
     3001 agccgatctc gggcgtggct gtcgaggacg cgccagcaaa ggcaggctgg aagccgctct
     3061 tggccgccgg cgcaccgttc ttgcgcgtga ccatccggta aatgaagaac gcgacgccag
     3121 cgaacagcgc aaccatcagc atcgtgctga tgatgctggc gagcgcaccg ccgataccga
     3181 gatgcgacag cagggcgccg aggccgaggc cgagcagcgc gccgccgagc aggcccttcc
     3241 acatgctggg cttggctgcg ttggcagcgg cgccggcacc ggctgcattg gttggttgtg
     3301 cctggcgctg ggccggggcg gcgttctgcg ccggcggcgt ggcctgacgg ctgacattgt
     3361 tcgattgctt gccgaaggag ccgccaccac ccatgcggcg cgcttccgcc tcaggcaaca
     3421 ccatcgacag ggaacccacg gcaatcatca gcgtgagcag gtactttttc atgtttcctc
     3481 ctttggaatg cgtcttgcca cggccacatc ggtcgcgcgc gggcgcaatg gcggatacta
     3541 tgccacacgc aaactttgaa aaagcgaagt taagaggatg aagtgtcctc tttttccgaa
     3601 gatcagtatt tcgccggtcg cagcacctgc acgtcggcga aatacatcgt cacgctgtaa
     3661 tgcgcggcgt agttggtcaa aacgtggtgg gcgatcgtgt ccgccaccga ttcctcgcac
     3721 acgacctcca tgtggatcgt gcggtcggct tccacgatgc cttcgcgcac gccggcggtg
     3781 ccgccgccgc gcacgtcgaa aatcgtgtag ccgtgcgccc cgaagcgctt gacgtcctgg
     3841 atcaggcgct tttccagcgc ggcctcggcg atgatgacga gcagctggcg cggatatttg
     3901 tcgagcttca tggcggcttt catgcggtaa gtgcctgggc aagccggatg tagagcggta
     3961 tgccgaacac aatgttgaag gggaaggtga tgcccagcgc aatcgtgatc gacagcgcgg
     4021 agttggcctg cggcaccgcg atgcgcatcg cggtcggcgc ggcgatgtag ctcgcgctcg
     4081 cggtcagcgt ggcgaggatg gccacgccgc cgaccgacag cccgagcgca acgccgacca
     4141 gcgcgccgag caccgccagc accggcggca cgcccaggcc gatcgcgaac gcgaccagcc
     4201 catagcgcct gacctcggcg aggcgaccgc cggcgaccag gcccagttcc agcaggaaca
     4261 gcgcgagcac gcccttgaac gggtcgatga acaccggcgc gatcggcgcg gtccccgcga
     4321 cgccggcaac cgcgccgatg atcagcccgc cgagcagcag gatcacgctc ttgccgatca
     4381 gcacatcgtg caggatcgcg ccccacccgg tcggcgcgcc acccgcgccc tgattccgcg
     4441 cgaacatgcg cgccagcagc acgccggcga tcaggcccgg catttccatc accgccaccc
     4501 acagcggcgc atgggcttcg tacggaatgg cggcatcgtt gagcttggcg atcaccaccg
     4561 cgaaggtcac cacgctgacc gagccgtaat gggcgccgat cgccgcgctg tcggccgcgt
     4621 tcaggcgcag cagacgaaac agcggataca gcgcaaacgg tatcgcaaga ccgagcagca
     4681 tcgtgacgcc gacctgaggc agcagggcaa gaagcggctg cttggacagc tcgatgccgc
     4741 ccttcaggcc gatcgcgagc agcaggtaga tcgacagcgt ttcatacagc gcttccggca
     4801 gccgcaggtc cgatttgacg agacgtgcga acacgccgag caggaagaag aagacgacga
     4861 cgtcgatcat gatgtgggcg ggaggaaaaa gtgcggccat tatcctacag ccgtgaccgg
     4921 cgttgccatg atggaggctg catgccgggc ccagaacttg caccccatct tgcgtgtcgt
     4981 acagataatt tttccccacc gcgagaaccc catggcccgc accctctgga aaggcgcgat
     5041 cagctttggc ctgctgcatg cgcccatcgc cttgtatacg gccgccaggt cggaggctgt
     5101 cgacttcgat tggctgcacc gcgagacgct gcagccgatc ggctacaagc gcgtgatcaa
     5161 ggaaaccggc gaggaagtag acaaggaaga catcgtcaag ggcgtcaagt acggcgacca
     5221 gtacgtgatc ctgtcggacg aggaaatcaa gtcggccaac gtcgagtcga cgcacacgat
     5281 cgacatcgtc ggcttcaccc gcgccgagga cgtcaatttc ctgtttttcg aaaagcccta
     5341 ctacctcgcg ccgaccaagg gcggggagaa ggtctacgtg ctgctgcgcg aggcgctgct
     5401 caagaccggc cgcgtcggcg tggcgctggt ggtgctgcac aacaagcagc acctggcggc
     5461 gctgatcgcc accccgcacg cgatggtgct caataccctg cgctgggcga gcgaagtcag
     5521 tgacgccagc gaactcaata ttcccgccga gggcatgaag gccgcgaaga tcaagccgaa
     5581 ggaagtcgag atggcgaccc agctgatcga aagcatgtgc gaccgctggg acccggagca
     5641 gtaccgcgac accttccgag acgacatcat ggcgctggtc aagcagaagg ccgatgccgg
     5701 cctggcgcag acgatcaccg aggtgaaggc cgaggagccg aaggaagagg ccgaggaagt
     5761 cgacctgacc gaactgctgc gccgcagcct gcagggcaac ggcaaggctg cacgaacgtc
     5821 cgcccgcaag gatgacggca agaaggcgcc gccgccggcg gcgcgcaaga gcgccgggcg
     5881 cacccgcaca tcgaaagccg cgcattgatc catggcgcac gatctcgtca cctaccacgc
     5941 caagcgcaat ttctccgtca cgcccgagcc gcgcggcgag cttgtcgccc ccggcgagcg
     6001 gctgcgcttc gtcgtccaga agcatcatgc acgccgcctg cactacgact ttcggctgga
     6061 gatcgccggc acgctcaaga gctgggcggt gccgaagggg ccgagcctgg acccggggca
     6121 gaaacgcctt gccgtgcatg tggaagacca tccactcgac tacatcgact tcgagggcga
     6181 catcccgccc ggccagtacg gggccggcca cgtgatcgtc tgggataccg gcagctggga
     6241 gccgctcggc gatccgcagg ccggctacga ggccggacgg ctgcgcttca gactcgacgg
     6301 cgaaaagctg cacggcgcgt ggaagctggt gaagaccggc atgcgcggca gcggcgacaa
     6361 ggaacagtgg ttcctgatca aggagaacga cgaggaggcg cgcagcactt ccgagtacga
     6421 catcgtcgag gccgaaccgg acagtgtcct cggcagcaag ggcgcaaagg caaaatcgcg
     6481 cgccgcgccg aagaagaagg cggcacgccc gcgggcacgc gaggaggaaa gcgccccggg
     6541 actggaagcg ctgcccggtg ctcgcctggc gcccctgccc ggtcgcatca cgccgcagct
     6601 ggccaccctg gtcgatgcgc cgccgaaggg cggcgactgg ctgtacgaga tcaagtacga
     6661 cggctaccgc gtgatggcct gcgtcgagaa cggcaaggcg cgcctgtaca cgcgcgacgg
     6721 ccacgactgg agcgcacgca tgccgacgca ggtggaggcc gtcgaggcac tcgggctgga
     6781 caatgcgtgg ctcgacggcg agatcgtcgt cctcgacgcg cacggcgcgc cttcgttcca
     6841 gctgctgcag aacgccttcg accgcaatgg cacggcgcgc atcatctact acctgttcga
     6901 cgtgccttac ctgaacggcg tggacctgcg caagactccg ctgcagctgc gccgacgcgt
     6961 cctcgcgcat ctgctcgagc atcacgacag cgacatcctg cgcttttccg cgcagctgga
     7021 ccagtcgccg cagcggctgc tggaccgggc ctgcgaaatg cacctggagg ggctgatcgg
     7081 caagcgcgcg gactcgatct acgtgagcgc gcgcagcagc agctggatca agctcaagtg
     7141 caggcggcgc caggaaatga tcatcgctgg ctataccgag ccgcagggcg ggcgccagta
     7201 cttcggttcg ctggtgctcg cggtgcacgc ggacgacggc agcctgcgct acgccggcaa
     7261 ggtcggcagc ggcttcgacg acgccgcgct ggccgacctg taccgccgga tgcagccgct
     7321 cgagcaggac gcctgcccgt tcgacaagcg ccctgcgggt ctgcgcggcg tgcactgggt
     7381 gcgcccgaag ctggtggccg agatcgcgtt cgcggaatgg accggcgaag gcgtgttgcg
     7441 gcaggcctcg ttccagggcc tgcgcggcga caagccggcg gccgaagtgc ggcgcgagca
     7501 ggccacccat atcaccgccc gcccgcgcgc gcgcaagccg gccgatcccc ttctcgaagg
     7561 cgtgaagatc accaatcccg agcgggtgat cgatgcggcc agcggcgcga ccaagctgga
     7621 gcttgcccac tactatcgcg agatcgcgcc gctggccctg cctttcctgc agaatcgccc
     7681 tgtctacctg ctgcgcgcgc ccgaaggcgt gggcggcgaa catttcttcc agaagcacaa
     7741 tacgcgcatg acgatcccag gcgtgcgcaa gctgcagatc gaactgattt ccgggcgcgc
     7801 gccgctgatg gcgatcgaaa cggccgaggc cttggttggc gccgcgcaga tgggcacgat
     7861 agaactgcac atcccgaacg tgaccgcgga ccgtgtcgag cgtcccgact gcatggtgtt
     7921 cgacctcgac ccggatccgg atctgccatg ggaggcgatg caggacgcgg cgcggctgac
     7981 gcgggtggtg ctcgacgaac tcggcctgac cagtttcctg aagaccagcg gcggcaaagg
     8041 cctgcacctg gtggtgccgc tgtcgcgccg gcatgactgg gatacgatgc acggattcgc
     8101 gcaggccgtc gcccagcaca tggcgcgcac gctgccgcag cgcttctccg cgaagatggg
     8161 cgcgaagaac cgcgtcggga agatcttcat cgactacctg cgcaacggcc gttcggccag
     8221 cacgatcgcg ccctactcgg tgcgcgcgcg gcccgggctg ccggtgtcgg tgccggtggc
     8281 gtgggaggag ctggacacgc tgaagcgttc cgggcagtgg accatcgtcg acctgccgga
     8341 actgctggag cgggcggaga acgacccgtg gaaggcgtac ttcgagacgc cgcagaacct
     8401 gaccgcggcg atgcgcagga agctggggat gaagtaggcg tgcccttaac ccaccagcag
     8461 cctcaccact cccgcaaagc tcaggaacga cagcacggtg gacaccatga tgatgcgcgc
     8521 cacccgttca ttgttcgcgc cgaagcgctc ggccagcagc gcgacgttgc tcgcgctggg
     8581 caaggacgcg accaggatca gcaccatgta ggcgaacggg tctagcggca tgccgagccg
     8641 gatcgccgcc gcaccgacca gcagcaccag caacggatgc agcaccagct tgaacagcgc
     8701 aaccggcaca tagtcggaca agcgcaacga agtggcgccg gtggcaggtg agcgcgccag
     8761 caccgcgccg atggtaaaca gcgcgaccgg cgacgcggca ttggcgagca gttctatcgt
     8821 gcggtcgatc ggccccggca ggccgaactc ggcggccgag gcgatcacgc ccagcacgat
     8881 ggcccagggc ataggattgg cggcgatgct cttgatcgcg ttcttcagcg ccgcgcgcac
     8941 gccgttcgcg ccggcgtcat ccatgcgcga caacgcaatg cacagcgagc tggtgatcac
     9001 catgtcgatc gcgatggtga tgatcaccgg accggccgcg cgcgcgccga tcagcgccag
     9061 cagcagcggg agtcccatga agccggtatt cgggaaggcc gcgaccagtg cgccgaacga
     9121 cgcgtcgttc cagttgatgc gcgcactcct ggtcatcgcg atcgtaaacc ccaccatcac
     9181 cagcgcgcac agcagccaga ccgcagccac gctggcgtcg agcagctgac cgatcggcga
     9241 agaggcgcca aagcggtaca gcatgcacgg cagcgcgaaa tacagcacga aggtattgag
     9301 cgccggcacc gcggtcgacg gcaggaagcc gcggcgcgcg gcgaggtatc ccgtagccac
     9361 cagcgcaaag aacgggaagg tcgtgtggaa gatcgagagc acggcaggct tgcgggagtg
     9421 atgggcacag gcgcgcgcct gtggtcgcgc aacggttgcg ttgcggaagt gactataccc
     9481 gaatcatttc cgggccggct gccctgaagg ggaatgccat gcacttggga gtgctaccgc
     9541 aaccctgtcg cccctgcgca cgcgggggcc cagctcgcgc tattgcaccg ttgcagacga
     9601 ttgtgccggg tcccccgcat gagcgggggc ccgcctaggg ggacgacaaa tcatcgctca
     9661 agcgatcggc atctcgcgca caggtacaca caggttgcac gccatccgtg caacctgcgg
     9721 cctcagtgga tgctcggctg acccggaccg ccggaagatg gcgaagacgg gttggcgccc
     9781 tccttcgcat ggccgtttcc ttgtgcgcgc gcctgggtat cctgggcgac ccgctggcct
     9841 tcctgtttcg cggtctcggc cacgcgccgg gccgactcct tcacgtgctc ggcctcttcg
     9901 cgtccgactt ccttggcgcg gtccagcatg tcgtcacggg tgcggcccag catctcgtcc
     9961 tcgcggcggg ttggcggcag cgcagccccg agcgcggcgc cgatggcgat gccgagcgcg
    10021 ccgagcacca gcggctggtc ttcgaacaga tgggagatgc tgtcgcgggc gcgatagtac
    10081 tgctcctgcg agcgatggcc gagatcctcc atccgcgcgc gcgcaccggc gccggaaccc
    10141 tgcatgcgcg agcgcatctg ctcgcccccg tgacgtacgc ggtccatcat gccggaggcg
    10201 gcatcgctga tgcggtgccc catctcgccg gccttgtcag ccatctggtg gccggtttcg
    10261 ctcatccggt tcatcgcctc gcggctggct ccgctcacgc gctccttcgc gtcgcggccg
    10321 gcctcggcgg cacgctcggc catgtcgtgc atgcggtcgc cctgtgtgcc gccgtgcagg
    10381 tcggagcgcg aatagtcgag gccgtagccg ccttcatagc cttcttcgat gccggaatcg
    10441 ctggcatacg ggtcatagcg ccgcccgcgt tccatcccgc cgccgcgctg tccctgcgcc
    10501 atcagccagg ccaggcccac gccgatcagc gcgatcggaa tcgggttgtc gcgcaccgcg
    10561 ttgccaaggt tcttgccgaa gtcggccggc aggctggtac gtgcataat
//
LOCUS       DATLGF010000189         2164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_54888,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000189 DATLGF010000000
VERSION     DATLGF010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54888"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VM406_05750"
     CDS             <1..401
                     /locus_tag="VM406_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HVL75501.1"
                     /translation="LCWATYGVLAQRWKIQPGAAAALVAVLSMLCYLPVYLLAGHAAL
                     AQGPAGEILLQALIQGAIVGGASVFVYSTAVRVLGATSTALFTAAVPCVTTLAAMPLL
                     GETPSAAALAGVGIVTAGLVAASASRSRRG"
     gene            541..1728
                     /locus_tag="VM406_05755"
     CDS             541..1728
                     /locus_tag="VM406_05755"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HVL75502.1"
                     /translation="MNDPIVILGAARTPIGAFQGAFSTLSANELGAVAIRAAIGRAGI
                     EASAVQEVVFGNCLIAGQGQAPARQAAIKAGLPFSTGAVTLSKMCGSGMKALMQAHDS
                     IVAGTNDIVAAGGMESMTNAPYLLPKARGGYRLGHGTIVDHMALDGLEDAYQKGQAMG
                     VFAEMCAAQYRFTRADQDEYALESVRRARQAAEEGAFGWEIAPVGVESRTGTVVVDAD
                     EGPTKARPEKIPQLKPAFAADGTITAASSSSINDGAAALILTRASRAQALGLKPIARI
                     LGHASHSQEPEWFTTAPVGAMRKLMQKLGSRERDFDLFEVNEAFATVPMAVMQEFDIR
                     HERLNVHGGACVLGHPIGASGARIVVTLIGALRRRGLQRGMAALCIGGGEGTALALEL
                     ELA"
     gene            complement(1756..>2164)
                     /locus_tag="VM406_05760"
     CDS             complement(1756..>2164)
                     /locus_tag="VM406_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HVL75503.1"
                     /translation="GMARYGLTGHAMQVFSAMRDAVLYMDQTRMPELFCGFERRPDEG
                     PTLYPVACSPQAWSAATVFSLLQACLGLSFDPQRREIRFRHPQLPPFLDTVEIRELQV
                     NGATVDLRLQRYPNNVGVNVVRKSGDVEVVVVA"
BASE COUNT          346 a          721 c          746 g          351 t
ORIGIN      
        1 cgctgtgctg ggcgacctac ggggtgctcg cgcaacgctg gaaaatccag ccgggcgcgg
       61 ccgccgccct ggtcgccgtg ctctcgatgc tctgctatct gccggtctac ctgctcgccg
      121 gccacgccgc gctcgcgcag gggccggccg gcgagatcct gctgcaggcg ctgatccagg
      181 gtgcgatcgt cggcggggcg tcggtctttg tctattccac ggcggtacgg gtgctcggcg
      241 ccaccagtac cgcgctgttc accgccgcgg tgccttgcgt cacgacgctt gcggccatgc
      301 cgcttctcgg cgaaacgcct tccgcggcgg cactggctgg cgtgggcatc gtcaccgccg
      361 ggctggtagc ggcctccgct tcccggtccc gtcgcggctg agtgcggatg ggtcattggc
      421 aatgccgtgt ctcgcgtctc tcgcagtcta tattacgatc atcatttcaa gcgacgcagc
      481 gcggctgcga ccctctcacc agcggcgctg cggcaacttt cacttcgaca ggagcctgcc
      541 atgaacgacc ccatcgtgat cctcggtgcc gcgcgcacgc cgatcggcgc cttccagggc
      601 gcattctcca cgctgtccgc gaacgaactg ggtgcggtcg caatccgggc agcgatcggc
      661 cgcgccggca tcgaagcgtc ggcggtgcag gaagtcgtgt tcggcaactg cctgatcgcg
      721 ggccagggac aggccccggc gcgccaggcg gccatcaagg ccgggctgcc gttctcgacc
      781 ggggctgtca cgctgtcgaa gatgtgcggt tcgggcatga aggcgctgat gcaggcgcat
      841 gattcgatcg tcgcaggcac gaacgacatc gtcgccgccg gcggcatgga atcgatgacg
      901 aacgcgccat acctgctccc caaggcgcgc ggcggctacc ggctcggcca cggcacaatc
      961 gtcgaccaca tggcgctgga cggacttgag gatgcttacc agaaagggca ggcgatgggg
     1021 gtgttcgcgg agatgtgcgc ggcgcaatac cggttcacgc gcgccgacca ggatgaatac
     1081 gcgctggagt cggtacgccg cgcccgccag gcggcggaag aaggcgcgtt cggatgggaa
     1141 atcgcgccgg tcggcgtgga aagccgcacg ggaacggtgg tggtcgacgc cgacgaggga
     1201 ccgacgaagg ccaggccgga gaaaattccc cagctcaagc cggccttcgc cgccgatggc
     1261 acgatcaccg ccgcgtcatc gtcgtcgatc aatgacggcg cagcggcact gattctgacg
     1321 cgcgcgtcgc gtgcgcaggc gctgggcctc aagccgattg cgcgcatcct cggccatgcc
     1381 tcgcattcgc aggaaccgga atggttcacc acggcaccgg tcggcgcgat gcgcaagctg
     1441 atgcagaaac tgggcagccg cgagcgcgat ttcgatctct tcgaagtgaa tgaagcgttt
     1501 gccaccgttc caatggccgt gatgcaggaa ttcgacatcc ggcacgagcg gctcaatgtg
     1561 cacggcggcg cctgcgtgct cggtcatccg atcggcgcat ccggcgcacg gatcgtcgtc
     1621 acgctgatcg gcgcattgcg ccgccgcgga ctgcagcgcg gcatggcggc gctgtgcatc
     1681 ggcggcggtg aggggacggc gctggcgctg gaactggaac tcgcataggg ccgggctgaa
     1741 caagtccttg cgcggctacg ccaccaccac cacttccaca tcgccgctct tgcgcacgac
     1801 gttcacgccg acattgttcg gataacgctg cagcctcagg tccacggtgg cgccgttgac
     1861 ctgcagttcg cggatctcga cggtatcgag gaagggcggc agctgcgggt ggcggaaacg
     1921 gatttcgcgc cgttgcgggt cgaacgacag gcccaggcag gcctgcagca gcgagaacac
     1981 cgtggccgcg ctccaggcct ggggcgagca ggccaccgga taaagcgtcg gtccctcgtc
     2041 cgggcgccgt tcgaatccgc agaacagttc cggcatgcgc gtctggtcca tgtagaggac
     2101 cgcgtcgcgc atcgcactga acacctgcat cgcgtgtccg gtcagcccgt agcgcgccat
     2161 gccc
//
LOCUS       DATLGF010000190         5426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_164430, whole genome shotgun sequence.
ACCESSION   DATLGF010000190 DATLGF010000000
VERSION     DATLGF010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164430"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VM406_05765"
     CDS             complement(<1..411)
                     /locus_tag="VM406_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HVL75504.1"
                     /translation="MLTTDKIQKLGLSSKLLFGASKKTRIVSVTALFLAACAFGAAGV
                     APMAPDPADIPVRAITEELSIPDLSEQIARLQEDEQTYLREERVRPGDTLGTLLNRLG
                     VDDSEAASFIRSDRTAQSVMQLRPGRRIQAETSGD"
     gene            696..1949
                     /gene="tyrS"
                     /locus_tag="VM406_05770"
     CDS             696..1949
                     /gene="tyrS"
                     /locus_tag="VM406_05770"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HVL75505.1"
                     /translation="MSATAPAQHTPSTQPLPLSSSVLEALATIKRGVDELLIEAEFAQ
                     KLARAEQTGTPLRVKLGLDPTAPDLHLGHTVVLNKLRQLQDLGHTVIFLIGDFTSMIG
                     DPSGRNVTRPPLTRAQIEENAKTYFAQASLVLDPERTEIRYNSEWCDALGARGMIELS
                     ARYTVARILEREDFAKRLKSGSPISVHELLYPLMQGYDSVALRSDLELGGTDQKFNLL
                     VGRELQKDYGQEPQCILTMPLLEGLDGVEKMSKSKGNYVGITEPANTMFGKLMSISDE
                     MMWRYYDLLSFRSNAEIAAFRKDVEQGRNPRDIKVLLAQEIVTRFHSARAAEEALADF
                     NNRARGGIPDDVPEVALSGAPLGIGQLLKQANLCPSSSEALRMVEQGGVRIDGEPVSD
                     KSLKVDAGTFVVQVGKRKFARVTLS"
     gene            1955..2434
                     /gene="dtd"
                     /locus_tag="VM406_05775"
     CDS             1955..2434
                     /gene="dtd"
                     /locus_tag="VM406_05775"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HVL75506.1"
                     /translation="MIALLQRVSRASVVVEGETVGAIGAGLVVLLCAERGDSEKEGEA
                     LLAKLLSYRVFSDAAGKMNRSVRDVEGGLLLVPQFTLAADTRSGTRPSFTPAASPEEG
                     RRLFDRFVERARAQHGKVETGRFGAHMELALVNDGPVTFWLQIKPPLGVSAVSSEAR"
     gene            2480..3130
                     /locus_tag="VM406_05780"
     CDS             2480..3130
                     /locus_tag="VM406_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HVL75507.1"
                     /translation="MKLWSDSFKDGAVIPGEFAFAVIDPNSHVALSSNRNPHLAWSDA
                     PVGARSFALICHDIDVPSRGDDVNQEGKRVPPDLPRVEFFHWVLVDLPAETSSIAAGQ
                     HSEKVTPRGKPGPELGAGLAGRHGLNDYTSWFAGDADMAGDYFGYDGPCPPWNDTIVH
                     RYIFTLYALDVEHLPVDGKFTGQQVRQAMQGHILAEAKIMGTYTLTPELAGKVTTS"
     gene            3127..3780
                     /locus_tag="VM406_05785"
     CDS             3127..3780
                     /locus_tag="VM406_05785"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HVL75508.1"
                     /translation="MTEILIIRHGETAWNAVRRLQGHLDIPLSAEGERQAAALAHALR
                     DEPLDAIYSSDLRRARQTAEAVARARGMTIIVEPGLRERSFGGFEGLLYAEIGARYPA
                     EFAAWKAREFDAGLPPGERAGETLREFFERTVGAVRDILMRDRHRRVAMFTHGGVLEC
                     VHRAAQGLRPDRPRDFDILNAGINRLHWDGNRLHLLSWGEVEHLRAERLAALDEIDR"
     gene            3783..4799
                     /gene="dusB"
                     /locus_tag="VM406_05790"
     CDS             3783..4799
                     /gene="dusB"
                     /locus_tag="VM406_05790"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HVL75509.1"
                     /translation="MQIGPYVLRNNVFVAPMAGVTDRPFRQLCKELGAGYAVSEMAAS
                     DPRLWASEKTSRRINHDGEMEPKAVQIAGADPAMLAASAKFNVERGAQIIDINMGCPV
                     KKVCNSWCGSALLQNETLVAEILRAVVSAVPVPVTLKFRTGWNRENRNALRIARLAED
                     AGIAMLTLHGRTRADGYSGEAEYETIAAVKAAVSIPVVANGDITTPHKARQVLAQTGA
                     DAVMIGRAAQGRPWIFREIDHFLRTGDLLPPPLVSEVRALMDAHLRAHYAFYGEYMGL
                     RTARKHIGWYVRDLPGGEDFRQRMNRLESCEEQLAAVDAFFESQKAWGERLQYAFAAE
                     KLAA"
     gene            4829..5062
                     /locus_tag="VM406_05795"
     CDS             4829..5062
                     /locus_tag="VM406_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HVL75510.1"
                     /translation="MSKDNIQDVVKKSLEKYFKDLGEQQPTNVYEMVVFTVEKPILEV
                     VMARAGGNQSLAADMLGINRNTLRKKLQQHGLL"
     gene            5122..>5426
                     /gene="purH"
                     /locus_tag="VM406_05800"
     CDS             5122..>5426
                     /gene="purH"
                     /locus_tag="VM406_05800"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869829.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HVL75511.1"
                     /translation="MIKQALISVSDKTGVLDFAKALSALGVNILSTGGTAKLLADNGV
                     KVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRELPEHMASLAQHGIPTIDMVVVNL
                     "
BASE COUNT          975 a         1700 c         1805 g          946 t
ORIGIN      
        1 gtcgccgctt gtttccgcct gaatgcgacg acccggtctc agttgcatca cgctttgggc
       61 ggtgcggtcg gagcggatga agctggcggc ttcgctgtcg tccaccccaa gccggttgag
      121 cagggtgcca agggtgtcgc ccggacgcac gcgctcttcg cgcaggtagg tctgctcgtc
      181 ttcctggagg cgggcgatct gttcggacag gtcgggaatg gacagttcct cggtaatggc
      241 ccgcaccgga atgtcggcgg ggtccggggc catcggggca acgcccgccg cgccgaatgc
      301 gcaggcagcg aggaacaatg cggtgacgga aacgatgcgc gtctttttcg acgcgccgaa
      361 caacagcttg ctgctcaggc ccagcttttg gattttgtct gtagtgagca tgcaatacgt
      421 tagaatttgc tgtttttcgc ggaagagatg ctcgtttcag ccacttgcaa tccctgccgc
      481 gatcggtcaa atccggcgtg gcgccggctc tgacgccgca aaaattctcg accagctgcg
      541 tgcgcatgcc ggtcgattgg cggttcacag gagtcggcag gtagacctgc tttattctat
      601 gaaagattgc caggacgcaa ggccctggca ggcagggatt ataccaaagg catgctaccg
      661 gcctttcaca ttcgcacatt aatttttgtc agcccatgag tgctactgct ccagcgcaac
      721 acaccccctc cacacaaccg ctccctctct ccagttcggt ccttgaagcc ttggcaacaa
      781 tcaagcgcgg cgtcgacgaa ttgctgatcg aggccgagtt cgcgcagaag ctggcgcgcg
      841 ccgagcagac cggcacgccg ctgcgcgtga agctgggcct ggacccgaca gctccggacc
      901 tgcacctggg tcacacggtg gtgctgaaca agctgcgcca gctgcaggat ctggggcaca
      961 cggtgatttt cctgatcggc gatttcacct cgatgatcgg cgacccgtcc gggcgcaacg
     1021 tgacgcgccc gccgctgacg cgcgcgcaga tcgaggagaa cgcgaagacg tatttcgccc
     1081 aggcgagcct cgtgctcgac ccggaacgca ccgagatccg ctacaactcc gagtggtgcg
     1141 atgcgctcgg cgcgcgtggg atgattgagt tgtcggcgcg ctacaccgtc gcgcgcatcc
     1201 tggagcgcga ggatttcgcc aagcgcctga agtccggctc cccgatctcg gtgcatgaac
     1261 tgctgtaccc gctgatgcag ggctacgact cggtggcgct gcgctcggac ctggagctgg
     1321 gcggcaccga ccagaagttc aatctgctgg ttggccgcga attgcagaag gattacggcc
     1381 aggagccgca gtgcatcctg accatgccgt tgctggaggg gctggacggc gtagagaaga
     1441 tgtcgaagtc gaaaggcaat tatgttggca tcacggagcc ggcgaacacg atgttcggca
     1501 agctgatgag catctccgac gagatgatgt ggcgctacta cgacctgctg tcgttccgct
     1561 cgaatgccga gatcgccgcg ttccggaagg acgtcgagca gggacgcaat ccgcgcgaca
     1621 tcaaggtgtt gctggcgcag gaaatcgtca cgcgcttcca ctcagcccgc gccgccgagg
     1681 aagcgctggc ggacttcaac aaccgggccc gcggcgggat tccggacgat gtgccggagg
     1741 tcgccctctc gggcgcgccg ctcggcatcg gtcaattgct caagcaggcg aacctctgcc
     1801 cctcctcttc ggaggcgttg cgcatggtcg agcagggcgg ggtgcgcatc gatggcgagc
     1861 cggtgagcga caagagcctg aaggtcgatg ccggcacctt cgtcgtgcag gtcggcaagc
     1921 gcaagttcgc ccgcgtcacg ctgagctgag gcgcatgatc gctttgctcc agcgggtgag
     1981 ccgcgcatcg gtcgtggtcg aaggagaaac ggtcggcgcg atcggcgccg gcctcgtggt
     2041 cctgctgtgc gccgaacgcg gcgacagcga gaaggagggc gaggcattgc tcgccaagct
     2101 gctgtcctac cgggtgtttt ccgacgcggc cggcaagatg aatcgcagcg tacgcgatgt
     2161 cgagggcggt ctgctgctgg tgccgcagtt cacgctggct gccgacacgc gttccggcac
     2221 ccggccgtcg tttacgccgg ccgcgtctcc cgaggaaggg cgccgtctgt tcgaccgatt
     2281 cgtcgagcgg gcgcgtgcgc agcacggcaa ggtcgagacc ggccgtttcg gggcccacat
     2341 ggagcttgct ctggtgaacg acgggccggt cacgttttgg ttgcagatca agccgccgct
     2401 cggcgtctct gcggtatcca gtgaagctcg ctagactggc atggtccgca cgtcaacaac
     2461 cgacaacaaa ggagaccgca tgaaactctg gagcgattca ttcaaggatg gtgccgtcat
     2521 tcccggcgag ttcgcgttcg ccgtgatcga cccgaattcc catgttgcgc tgtcctcgaa
     2581 ccgcaatccg cacctggcct ggagcgacgc gccggtcggc gcgcgctcgt tcgcgctgat
     2641 ctgccatgac atcgatgtgc cctcgcgcgg cgacgacgtg aaccaggaag ggaagagggt
     2701 gccgccggat ctgccgcgcg tggagttttt ccattgggtg ctggtcgatc tgccggcgga
     2761 aacttcatcg attgccgccg gtcagcatag cgagaaggtg accccgcgcg gaaagccggg
     2821 gccggagctc ggcgccggcc tcgcggggcg ccatggcctg aacgattaca caagctggtt
     2881 cgcgggggac gccgacatgg ccggcgacta cttcggctat gacggaccgt gcccgccgtg
     2941 gaacgatacg attgttcacc gctacatctt cacgctgtat gcgctggacg tcgagcatct
     3001 gcctgtggac ggcaagttca ccggccagca ggtgcgtcag gcgatgcagg gccacatcct
     3061 cgccgaggcg aagatcatgg gcacctacac gctgacgccg gagctcgctg gaaaagtcac
     3121 gacctcatga cagaaatact catcatccgt catggcgaaa ccgcctggaa cgcggtccgc
     3181 cgcctgcaag gccacctgga catccccttg agcgccgagg gcgagcgcca ggcggccgcg
     3241 cttgcgcatg cgctgcgcga cgagccgctc gacgcgatct attcaagcga tctgcggcgt
     3301 gcgcggcaga ctgccgaggc cgtcgcgcgt gcgcgcggga tgacgatcat cgtcgagccc
     3361 ggcttgcgcg agcgcagttt cggcggcttc gagggcctgt tgtacgccga gatcggcgcc
     3421 cgctatccgg cggagttcgc cgcctggaag gcgcgcgaat tcgatgcggg cctgccgccg
     3481 ggcgagcgcg ccggggaaac gctgcgcgag ttcttcgagc gcaccgtggg cgcggtgcgc
     3541 gacatcctca tgcgcgaccg ccatcgccgc gtcgcgatgt ttacccacgg cggcgtgctc
     3601 gaatgcgtgc atcgcgccgc gcaagggctg cgtcccgacc gtccgcgcga tttcgacatc
     3661 ctcaacgcgg gcatcaatcg gctccactgg gacggcaatc gcctgcacct gctgagctgg
     3721 ggcgaggtcg agcacctgcg cgccgaacgc ctggcagccc tcgacgagat cgaccgctga
     3781 gcatgcagat cggtccttac gtattgcgca acaacgtgtt cgtcgcgccg atggccggcg
     3841 tgacggatcg tcctttccgc cagctttgca aggagctcgg cgccggctat gcggtgtcgg
     3901 agatggcggc atccgatcct cggctgtggg cgagcgagaa gacctcgcgc cggatcaacc
     3961 acgacggcga gatggagccg aaggcggtgc agatcgccgg cgccgatccg gcaatgctgg
     4021 ccgcgtctgc gaagttcaac gtcgaacgcg gggcccagat catcgacatc aacatgggat
     4081 gcccggtcaa gaaggtctgc aacagctggt gcggctcggc cctgctgcag aacgagacgc
     4141 tggttgccga gatcctgcgc gcggtggtca gcgcggtgcc ggttccggtg acgctgaaat
     4201 tccgcaccgg ctggaatcgc gagaacagga atgcgctgcg catcgcgcgt cttgccgagg
     4261 atgcgggcat cgcgatgctg accctgcatg gccgcacgcg cgccgacggt tattccggcg
     4321 aggccgaata cgaaacgatc gcggcggtga aggccgccgt gtcgattccg gtcgtcgcca
     4381 acggcgacat cacgacgcca cacaaggcgc ggcaggtgct tgcgcagacc ggcgcggacg
     4441 cggtgatgat cgggcgcgcc gcgcaggggc gtccgtggat tttccgcgag atcgatcatt
     4501 tcctgcgcac cggcgatctg ctgccgccgc cgctggtttc cgaggtgcgc gcgctgatgg
     4561 atgcgcattt gcgcgcccac tatgcgttct atggcgagta catgggactg cgcaccgcgc
     4621 gcaagcatat cggctggtat gtgcgcgacc tgccgggcgg cgaggacttc cgccagcgca
     4681 tgaaccggct ggagagctgc gaggagcagc tggccgcggt cgatgccttt ttcgaatcgc
     4741 agaaagcctg gggtgaacgg ttacaatacg cgtttgccgc cgaaaagctg gccgcctgac
     4801 cccgaaaaag aacagcatct ccatcgcaat gagcaaagac aacatccagg acgtcgtcaa
     4861 gaaaagcctt gagaagtact tcaaggacct gggcgagcag caaccgacga acgtctacga
     4921 gatggtggtc ttcacggtgg aaaagccgat cctggaagtg gtgatggcgc gcgccggcgg
     4981 caaccagtcg ctcgccgccg acatgctcgg catcaatcgc aacacgctgc gcaagaagct
     5041 gcagcagcac ggcttgctgt gatcgataac gcaagcggca cgggtgccgt ttgcgttatc
     5101 catcacatcc cgcaagccac catgatcaag caagccctca tctccgtctc cgacaagacg
     5161 ggcgttctcg atttcgccaa ggccttgtcc gcgctcggcg tcaacatcct ctccaccgga
     5221 ggtaccgcca agcttctggc tgacaacggc gtgaaggtga ccgaggtcgc cgactacacc
     5281 ggcttcccgg aaatgctgga cgggcgggtg aagacactgc atccgaaggt ccatggcggc
     5341 attctcgcgc ggcgcgagct gccggagcac atggcctcgc tcgcgcagca tggcatcccg
     5401 accatcgaca tggtggtggt caatct
//
LOCUS       DATLGF010000191         4948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_99926,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000191 DATLGF010000000
VERSION     DATLGF010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99926"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VM406_05805"
     CDS             <1..936
                     /locus_tag="VM406_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVL75512.1"
                     /translation="ADAGVQVETVGRTLETMLGGRNVTRFKRGSDQYDVIVQIEGDNR
                     SAPDDITNIFVRGAGDRMIPLSSLLNVRETVAPRELNHFGQRRAAIISANLANGYTQG
                     EAIAFMEETARRILQPGFSTDLAGSAREYRAASGSLMLTFVLALAFIYLVLAAQFESF
                     IDPFIIMLTVPLSMAGALLALYLTGGTLNVYSQIGLITLVGLITKHGILIVEFTNQLR
                     EQGKDIFEAVTEAAVLRLRPILMTTGAMVLGAVPLALATGAGAESRMQIGWVIVGGMS
                     FGTLLTLFVVPTFYTFLASKKAHAPLPASAPATPA"
     gene            939..1661
                     /gene="dnaQ"
                     /locus_tag="VM406_05810"
     CDS             939..1661
                     /gene="dnaQ"
                     /locus_tag="VM406_05810"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HVL75513.1"
                     /translation="MRQIVLDTETTGLNPRSGDRIIEVGCVEILNRQLTGNNYHVYIN
                     PERDSEEGALAVHGLTTEFLSDKPKFAEIAGELLDYVRDAEIIIHNAPFDLAFLDAEF
                     ERLGLPAFDRHVAGITDTLVQAKQMYPGKRNSLDALCDRYGVSNAHRTLHGALLDAEL
                     LAEVYLAMTRGQNSLTIDLMAAEETAEGTAADMPPIGEIIVLAASEEELAEHEQVLDR
                     LDKEVKGACVWRKPAAEAAPQA"
     gene            1709..1783
                     /locus_tag="VM406_05815"
     tRNA            1709..1783
                     /locus_tag="VM406_05815"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1741..1743,aa:Val,seq:cac)
     gene            1957..2556
                     /locus_tag="VM406_05820"
     CDS             1957..2556
                     /locus_tag="VM406_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HVL75514.1"
                     /translation="METIETILHFWFGTESDDARVASAQARLWWMKHPDVDREIRERF
                     EGTLEAAMRGELDGWRASPRGRLALIVLADQFPRNMYRDTPRAFAYDAQARGWCLEAL
                     DAGADQALRPIERVFLYLPLEHSERLEDQERAVALFKRLHEEAGEAARPAFAEFLDYA
                     YRHRDVIARFGRFPHRNRILQRASTDEEEAFLRTPGSSF"
     gene            complement(2722..>4948)
                     /locus_tag="VM406_05825"
     CDS             complement(2722..>4948)
                     /locus_tag="VM406_05825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HVL75515.1"
                     /translation="PQGIPPKVRTQIEHELVLIEQLKFEAYFLTVYDIVRFARSRGIL
                     CQGRGSAANSAVCYCLGITEVDPARGNMLFERFISKERNEPPDIDVDFEHQRREEVIQ
                     YVYEKYGRDRAALTAVVISYRPRSVLRDVGKALGVDLSIVDKVAKSHRWWDSKTELAE
                     RFAECGVDPASPVARQWEALARQMLSFPRHLSQHPGGFVIARGKLSRLVPIENAAMAD
                     RSVVQWDKDDLDAIGLLKVDLLALGMLSMIRRALDMISEQSGESFTMQDIPSEDPATY
                     AMISEADTVGVFQIESRAQMTMLPRLKPKRFYDLVIQVAIVRPGPIQGGMVHPYLRRR
                     QGLEPITYPSPEVRGALERTCGVPIFQEQVMQIAMAAAGFSAGEADQLRRAMAAWRRK
                     GDLGKFQEKLITGMSERGYSEEFIDAIVRQIHGFAEYGFPESHAASFALLAYASSWIK
                     RHHPAAFLAALLNSQPMGFYSPSQLVQDAVRHGVVVRPVDIVCSHWEAMLEPVPGEQP
                     AVRLGFNLVKGLSQESALRIEEARMIRPFASLHDLSVRAQLPRRELQALAHANALKAL
                     AGHRREALWHAVAGEADKDLLREAEITEEGVSLPPPSEGQDIVADYHAIGLTLGRHPL
                     ALLRPMLARRDFQPAEVLNTYRNGRRAHACGIVTVRQRPSTASGTIFVTLEDETGPVN
                     VIVWPRVGETYRKELLGARLLGVHGQWQCEGSVRHLVAQRLVDMSWMLGELMTRSRDF
                     A"
BASE COUNT          809 a         1623 c         1638 g          878 t
ORIGIN      
        1 gccgatgccg gcgtgcaggt cgagacggtg ggtcgtacgc tggagaccat gctgggcggg
       61 cgcaacgtca cccgcttcaa gcgcggcagc gaccagtacg acgtgatcgt gcagatcgaa
      121 ggcgacaacc gcagcgcgcc ggacgacatc accaatatct tcgtgcgcgg tgccggggat
      181 cgcatgatcc cgctgtcgag cctgctcaac gtgcgcgaga cggttgcgcc gcgcgagctg
      241 aatcacttcg gccagcgccg cgccgcgatc atctccgcca acctcgccaa cggctacacc
      301 cagggcgagg cgatcgcgtt catggaagag acggcaaggc gcatcctgca gcccggcttc
      361 tcgaccgacc tggccggttc cgcgcgcgaa taccgcgcgg catccggcag cctgatgctg
      421 accttcgtgc tggcactggc cttcatctac ctggtgctgg cagcgcaatt cgaaagcttc
      481 atcgatccgt tcatcattat gctgaccgtg ccgctatcga tggcgggcgc gctgctggcg
      541 ctctacctga ccggcggcac gctcaacgtg tacagccaga tcggcctgat cacgctggtt
      601 ggcctgatca cgaagcacgg catcctgatc gtagaattca cgaaccagct gcgcgagcag
      661 ggcaaggaca tattcgaggc ggtgaccgag gccgcggtgc tgcgcctgcg accgatcctg
      721 atgacgaccg gtgcgatggt gctgggcgcc gtgccgctcg cgcttgcgac cggagccggc
      781 gcggaatcgc gcatgcagat cggctgggtc atcgtcggcg gcatgagctt cggcacgctg
      841 ctcacgctgt tcgtcgtccc gaccttctac actttccttg cgagcaagaa ggcgcatgcg
      901 ccgctgcccg catccgcacc cgccacacct gcctgaccat gcgacaaatc gttctcgaca
      961 ccgaaaccac cggcctcaac ccgcgctccg gcgaccgcat catcgaagtc ggttgcgtgg
     1021 agatcttgaa ccgccagctc accggcaaca actatcacgt ctacatcaat cccgagcgcg
     1081 actcggaaga gggcgcgctc gcggtgcacg gcctgaccac cgagttcctg agcgacaagc
     1141 cgaagtttgc tgagatcgcc ggggaactgc tcgactacgt gcgcgacgcg gaaatcatca
     1201 tccacaacgc gccgttcgat ctcgcctttc tcgacgccga gttcgagcgg ctcggcctgc
     1261 cggctttcga ccggcacgtc gcagggatca ccgatacgct ggtgcaggcc aagcagatgt
     1321 atccgggcaa gcgcaattcg ctcgatgcgc tgtgcgaccg ctacggcgtc tccaacgccc
     1381 accgcacgct gcatggcgcg ctgctcgatg ccgagcttct ggccgaggtc tatctcgcga
     1441 tgacgcgcgg ccagaacagc ctgaccatcg acctgatggc ggcggaggaa accgcggaag
     1501 gcacggcagc cgacatgccg ccgattggcg agatcatcgt gctggccgcc agcgaggaag
     1561 aactggccga acacgaacag gtgctcgacc gcttggacaa ggaagtaaag ggtgcctgcg
     1621 tctggcgcaa gcccgccgcc gaagccgcgc cgcaggcctg atgtttccgt tgcgcccggt
     1681 tttttgctgt gccataatag cgcgcttcgg acggttagct caggggtaga gcactgcctt
     1741 cacacggcag gggtcactgg ttcaatccca gtaccgtcca ccagatttcc ccgaaccggc
     1801 tcgcattgcg agccggtttt ttttgcccgt tcatctcgcg tgccggacct tcgccggagc
     1861 gcaatgcgag ggagacatgg gtacgcgttg acgcatatgc ggtgtgtcct gcagctgttt
     1921 cgcttatgct gaagtttttt gccggcgcat tcttccatgg aaacgatcga aaccatcctg
     1981 cacttctggt tcggcacgga atcggatgac gcgcgcgtgg cttccgcgca ggctcgcctg
     2041 tggtggatga aacatcccga cgtggatcgt gagatccgcg agcgcttcga ggggacgctt
     2101 gaagcggcga tgcgcggcga gctggatggg tggcgcgcca gcccgcgcgg gcggctggcg
     2161 ctgatcgtgc tcgccgatca gttcccgcgc aacatgtacc gcgatacgcc gcgtgcattc
     2221 gcttatgacg cacaagcgcg tggctggtgc ctggaggcgc tggacgcagg cgcggaccag
     2281 gcgttgcggc cgatcgagcg ggtgttcctg tatctgccgc tcgaacattc cgagcggctg
     2341 gaggaccagg agcgggcggt ggcgctgttc aagcgcctgc atgaggaggc gggcgaggcg
     2401 gcgaggccgg cattcgccga gtttctcgat tacgcgtatc ggcatcgcga cgtcatcgcg
     2461 cgcttcggcc gtttccctca tcgcaaccgc atcctgcaac gcgcgtctac cgacgaggag
     2521 gaagccttcc tgcgcacgcc gggatcgtcg ttctaggttc tagggctacg ctgaacaagt
     2581 cctcgcgcgt cgcgcatccc gaactcgggc ggtctgcggc gttgcgaata ctcgcgatag
     2641 ccccgctatc gctgcgtctc gcgccttgca tcccatcccg atttcgggcg cgcaccatcg
     2701 cgggacttat tcagcgtagc cctaggcgaa atccctgctg cgcgtcatca gttcccccag
     2761 catccagctc atgtccacca gccgttgcgc gaccaggtgg cgcacgctgc cttcgcactg
     2821 ccactgcccg tgcacgccaa gcaggcgcgc gccgagcaat tccttgcggt aggtctcgcc
     2881 cacgcgcggc cagacgatca cgttgaccgg gccggtttcg tcttcgagcg tgacgaagat
     2941 ggtgccgctg gcggtgctgg ggcgctggcg cacggtgacg atgccgcagg cgtgggcgcg
     3001 gcggccgttg cggtaggtgt tgagcacctc ggccggttgg aaatcgcgcc tggccagcat
     3061 cggccgcagc aacgcgagcg gatggcggcc gagggtgagg ccgatggcat ggtagtcggc
     3121 aacgatgtcc tggccttcgc tgggcggcgg caggcttacg ccttcctcgg tgatttcggc
     3181 ctcgcgcagc aggtccttgt ccgcttcgcc ggcgaccgcg tgccacagtg cttcgcggcg
     3241 atggccggcg agcgccttga gcgcgttcgc atgggccagc gcctgcagct ctcggcgcgg
     3301 cagctgggcg cgtacggaca gatcgtgcag gctggcgaac ggacggatca tgcgcgcttc
     3361 ctcgatgcgc agcgcacttt cctgcgacag gcctttgacg agattgaagc cgagccgtac
     3421 ggcgggctgc tcgcccggca ccggttcgag catcgcttcc cagtggctgc agacgatgtc
     3481 gaccggcctc accacgacgc cgtggcgcac cgcgtcctgc accagttgcg agggcgagta
     3541 gaaacccatc ggctggctgt tgagcagggc ggcgaggaag gcggccggat gatggcgctt
     3601 gatccaggaa ctggcatagg cgagcagcgc gaagctggcc gcgtggcttt ccgggaaacc
     3661 gtattcggca aagccgtgga tctggcgcac gattgcgtcg atgaattctt cgctgtagcc
     3721 gcgctcgctc atgccggtga tgagcttttc ctggaatttc ccgagatcgc ccttgcgccg
     3781 ccaggccgcc atcgcgcggc gcaactggtc ggcctcgccg gcggaaaagc cggcggcggc
     3841 catcgcgatc tgcatcacct gttcctggaa gatcggcacg ccgcaggtgc gctccagcgc
     3901 gccgcgcact tccgggctcg gataggtgat cggctccagc ccctggcggc ggcgcaggta
     3961 ggggtggacc atgccgccct ggatcgggcc ggggcgcacg atcgcgacct ggatcacgag
     4021 gtcgtagaag cgcttcggtt tcaggcgcgg cagcatggtc atctgcgcac ggctttcgat
     4081 ctggaacacg ccgacggtgt cggcctcgct gatcatcgcg taggtggccg ggtcttcgga
     4141 cgggatgtcc tgcatcgtga aggattcgcc gctctgctcg ctgatcatgt cgagcgcgcg
     4201 ccggatcatc gacagcatgc cgagcgcgag caggtcgacc ttgagcaggc cgatcgcatc
     4261 gagatcgtcc ttgtcccatt gcacgacgct gcggtccgcc atcgcggcgt tctcgatcgg
     4321 caccaggcgc gagagcttgc cgcgcgcgat cacgaagccg cccgggtgtt gcgacagatg
     4381 gcgcgggaag ctgagcatct ggcgcgccag cgcttcccac tggcgcgcca ccggcgaggc
     4441 gggatcgacc ccgcattcgg cgaagcgctc ggccagctcg gtcttgctgt cccaccagcg
     4501 gtgcgacttg gccaccttgt cgacgatgga cagatccacg cccagtgcct tgccgacatc
     4561 gcgcagcacg ctgcgcggac ggtagctgat gacgaccgcg gtcagcgcgg cgcggtcgcg
     4621 gccgtatttc tcgtacacgt actggatcac ttcctcgcgc cgctggtgtt cgaaatcgac
     4681 gtcgatgtcg ggcggctcgt tgcgctcctt cgagatgaag cgctcgaaca gcatgttgcc
     4741 gcgcgccgga tcgacttcgg tgatgccgag gcagtagcac accgccgagt tggcggccga
     4801 gccgcggccc tggcacagga tgccgcgcga gcgcgcgaag cgcacgatgt cgtacaccgt
     4861 caggaaatac gcctcaaact tcagttgctc gatcagcaca agctcgtgct cgatctgcgt
     4921 gcgcaccttc ggcggaatgc cctgcggg
//
LOCUS       DATLGF010000192         8425 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_55029,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000192 DATLGF010000000
VERSION     DATLGF010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 8425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8425
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55029"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VM406_05830"
     CDS             complement(<1..894)
                     /locus_tag="VM406_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HVL75516.1"
                     /translation="MQTSPISNLSTVSNAPASAPDKQAGGFDKIFTREVASRKEAADA
                     SRGAERREAAPGREASAPSPNTPAAEAQPPAADTAAEETTGQPSADVLALVANMVQLA
                     QGEGQATEEAAADAAEADAVLPGARRRGTPFAPQHGAIGSRANHGTQSRPEGSWAAQV
                     ERGTSVRVEAGRTDAATAAQPLSAATASAKPAVAGASDFGAILKDAAALQAPQQQQFE
                     LQPANARQAVEHLSPRVGSPGWDQALGQRIVWMVNGEQQSASLTLNPPELGPLKVVLQ
                     MTSNQASASFVAAQPEVRQALE"
     gene            complement(1060..1500)
                     /gene="fliJ"
                     /locus_tag="VM406_05835"
     CDS             complement(1060..1500)
                     /gene="fliJ"
                     /locus_tag="VM406_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HVL75517.1"
                     /translation="MVKHSALDTLIELATNQTEEAARRLGTAIKQCADNEQRLAMLLQ
                     YRDEYETRLRQGMTAGISASGYRNFQLFLEKLDAAIRGQEQIVQTARHRITAERGAWQ
                     SSERTRVSYDTLAARAQQTALARQNKQDQKLTDELATRATTYKR"
     gene            complement(1530..2927)
                     /gene="fliI"
                     /locus_tag="VM406_05840"
     CDS             complement(1530..2927)
                     /gene="fliI"
                     /locus_tag="VM406_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HVL75518.1"
                     /translation="MSGHLDRWQAYLNDCRSLVAIAEPLQVSGRVTRVAGLVMEAVGL
                     KLAVGSACSIPLPSGARIEAEVVGFENDKLLLMPQNDVEGVLPGARVFPIEVTQSLPP
                     PGRVAHPRRRPSDRGRHLPVGEELLGRVVDGAGRPLDSLGPIHAAHSAPLNARPANPL
                     ARAPISQMLDVGVRSINSMLTVGRGQRMGLFAGSGVGKSVLLGMMARYTSADVIVVGL
                     IGERGREVKEFIEQILGPAGLARSVVVAAPADTPPLMRLQGAAYATAIAEHFRDQGKN
                     VLLIMDSLTRYAMAQREIALAIGEPPATKGYPPSVFAKLPTLVERAGNGREGGGSITA
                     FYTVLTEGDDQQDPIADSARAILDGHIVLSRSLAEAGHYPAIDVEQSISRAMHNIAAD
                     PHQKAARRLKQLYSRYQRNRDLINVGAYSAGSDPVLDEAIASIGKIEAFLQQGIHERA
                     GIAESLQGLSALFDR"
     gene            complement(2927..3574)
                     /locus_tag="VM406_05845"
     CDS             complement(2927..3574)
                     /locus_tag="VM406_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HVL75519.1"
                     /translation="MSSSVIPKEKLSAYERWELASFGDAPAPAPVDHAALAAQHEQAL
                     AEERAQARKQGHDEGFEQGRKAGLAAGRAEAAREAACLRQVVEAFSNDLNRANEQVAD
                     DLLHLSLDLAKAMLKTALKVRPELVLPVVAESIRYLPSVQQPALLVLHPADAELVRAH
                     LTDELDKAGWRIAEDAQMERGGCRVETASNQIEAGAPVRWQRIAEALGKQSDWLE"
     gene            complement(3752..4747)
                     /gene="fliG"
                     /locus_tag="VM406_05850"
     CDS             complement(3752..4747)
                     /gene="fliG"
                     /locus_tag="VM406_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HVL75520.1"
                     /translation="MSEDGIMKAAILMLALGEEEAAEVMRYLGPREVQKLGAAMSTMK
                     AVASGQLEKVLDDFRSETEVNTSFGMDSDEYIRSVLTRALGEDKAATLLNRILGTRDA
                     SGIESLKWMDGQSVADLVRNEHPQIIATILVHLERYHACEVLGYFTERLRNDVVLRIA
                     TLDGVQPAALRELNEVLTKLLTGNEALKKKPIGGVRAAAEILNFLSGDAEASVMENLK
                     SYDPDMAQKIMDEMFVFDNIIEIDDRGIQLLLREVQSESLIIALKGASQELREKIFKN
                     MSQRAAEMMREDLDAKGPVRLSEVEAQQKEILQIVRRLADEGQIILGAKGDDAFI"
     gene            complement(4740..6395)
                     /gene="fliF"
                     /locus_tag="VM406_05855"
     CDS             complement(4740..6395)
                     /gene="fliF"
                     /locus_tag="VM406_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HVL75521.1"
                     /translation="MSVAGETATAPTGLVGFAQTPGGRNFFLMLGVAAVLAVMSVIWM
                     WSKEPDYRVLFSNFSDRDGGAIVAALQQMNVPYKFADGGGAILVPSSQVHDVRLRLAS
                     QGLPKGGNVGFELMENQRLGVSQFLEQVNFQRALEGELARSIQSVAAVQAARVHLALP
                     KNSVFVRDQQKPTASVLLNLHPGRSLDEQQVSAIVHLVASSVPELPTKNVTVVDQNGN
                     LLSDKGKKGANTLDPTQLKYVQELQQSIVRRIESIITPIVGPGNVRAEATADVDFSHT
                     EQAAEIYTPNQAPGASAIRSQQSSESRTTTPNGAVGVPGALTNQPPAPATAPIVADAA
                     ATAPGAAPAAAPAPTNEQRDTTVNYEVDKTVRYVQQPMGGIRRLSVAVVVNFKRDFDA
                     EGKPIMRALSDDEKNQITDLVREAMGFNRERGDSLNVVNSPFAGVEPEVIEQPPLWKR
                     PEMLDLGMTAGRYMLGGILLLYLYFAFLRPLIRRIMKTFEPPQQPPAPQIEEDAVVQI
                     EHEPAPMGSRPRTYQDNLDAAKELARKDPKMVANVVKSWVGNE"
     gene            6630..6974
                     /gene="fliE"
                     /locus_tag="VM406_05860"
     CDS             6630..6974
                     /gene="fliE"
                     /locus_tag="VM406_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HVL75522.1"
                     /translation="MIDSGRIEAMIAQLRAAANPTANRPIAGVAETAAAEKVAPKLDF
                     AAALKNSLDQVNSAQRSSEALTQRFAMGDDSVSLSDVMINSQKASISFHATVQVRNKL
                     VSAYQDIMNMSV"
     gene            complement(7204..7971)
                     /locus_tag="VM406_05865"
     CDS             complement(7204..7971)
                     /locus_tag="VM406_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HVL75523.1"
                     /translation="MGFNSKFGTEDVSPYQVHSRREIIALLRAMFERNQLVSMQADGG
                     AEAVVTSVLEVDEENGIVVVDRAPTNLVNQRILDSENVSFETVLDNIRILFFANQVRE
                     CLYDNLPALYIPIPQSLVRLQRREHYRVPTPVASPLKCTITIPPDDAGSHGTTISVTV
                     KDISCGGIAIIDEKKLLDNTIGRSYEQCRLALPGGAPLAVKLQVRNSLDQTLTTGRTL
                     RKIGCMFVDLPTPMLAAIQRYITKLERERNAKATGMG"
     gene            complement(8016..8318)
                     /locus_tag="VM406_05870"
     CDS             complement(8016..8318)
                     /locus_tag="VM406_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HVL75524.1"
                     /translation="MAHPDAKRNAVALAYQTGDLAPKVVAKGRGLIADEIIARAREHG
                     VFVHESRELVALLMQVDLDRHIPPALYRVVAELLAWLYHIDAIKGGAIPPGSLPRE"
BASE COUNT         1236 a         2750 c         2816 g         1623 t
ORIGIN      
        1 ttccagtgcc tgcctcactt ccggctgcgc ggcaacgaag ctcgcgctgg cctggttgct
       61 cgtcatctgc agcaccacct tgagcgggcc gagttcgggc ggattcagcg tcagcgaggc
      121 cgactgctgc tcgccgttga ccatccagac gatgcgctgg ccgagcgcct ggtcccagcc
      181 cgggctgccg acgcgggggc tcaggtgttc gactgcctgg cgcgcgttcg ccggctggag
      241 ttcgaattgc tgctgctgcg gtgcctgcag cgccgcggca tccttcagga tcgcgccgaa
      301 gtcgctcgcg ccggcgaccg cgggcttcgc ggaggcagtg gcggccgaca gcggctgggc
      361 cgcggtggcg gcgtcggttc tgcccgcttc cacgcgcacg ctggtgccgc gctcgacctg
      421 ggccgcccag ctgccttccg gcctggactg cgtgccgtgg ttggcgcggc tgccgatggc
      481 accgtgttgc ggggcgaacg gcgtgccgcg cctgcgtgct cccggcagga cagcatcggc
      541 ctctgccgca tccgcggccg cctcttccgt tgcctggcct tcgccctgcg cgagctggac
      601 catgttggcg accagcgcaa gcacgtcggc ggacggctgc ccggtcgttt cctcggcggc
      661 ggtgtccgct gcaggcggct gcgcttcggc agccggcgtg ttcggagacg gtgcgctcgc
      721 ttcccggccc ggcgccgctt cccgccgctc ggcgccgcgc gacgcgtcgg cggcttcctt
      781 gcggctggcc acctcgcgcg tgaagatctt gtcgaatccg ccggcttgct tgtccggggc
      841 ggacgcaggc gcgttcgaaa cggtgctcag gttggagatc ggcgaagttt gcatggttag
      901 tgtcctgtcc cgcaaatatg tgcgcatgaa atcgcgcctt tcgggccatg cccgtgttgc
      961 aaatcctcgc gataccacac ggtatcgctg cggtttttgc cttgtcctgc cccgaaatgc
     1021 atcgatttca ttttgcgcac ctatttacgg gacagggcac tagcgtttgt aagtggtcgc
     1081 ccgggtggcg agctcatcgg tcagtttctg atcctgcttg ttctgccttg ccagcgcggt
     1141 ctgctgcgcg cgtgcggcca gggtgtcata ggagacgcgc gtgcgttcgc tcgactgcca
     1201 ggcgccgcgc tcggctgtga tccggtgcct ggccgtctgc acgatctgct cctgcccgcg
     1261 gatcgccgcg tcgagttttt cgaggaacag ctgaaagttg cggtatccgc tggcgctgat
     1321 tccggccgtc atgccctgtc ggaggcgtgt ctcgtattcg tcgcggtact gcagcagcat
     1381 cgcgagccgc tgctcgttgt ccgcgcattg cttgatcgcg gtgccgaggc ggcgtgccgc
     1441 ttcctccgtc tggttcgttg ccagttcgat cagggtgtcg agtgcggagt gcttgaccat
     1501 gatgcattat aggttggcat cgtttgcgct catcggtcga atagcgcgga aagtccctgc
     1561 aagctctcgg caatgccggc ccgttcgtgg atgccctgct gcaggaaggc ttcaattttc
     1621 ccgatcgacg cgatcgcctc gtccagcacc ggatcggagc cggcgctgta ggcgccgaca
     1681 ttgatcaggt cgcgattgcg ctggtagcgc gagtacagct gtttcagtcg gcgcgccgct
     1741 ttctggtgcg ggtcggcggc gatgttgtgc atggcacggc tgatcgactg ttcgacgtcg
     1801 atcgccgggt aatgcccggc ttcggccagg ctgcggctga gcacgatgtg tccgtccagg
     1861 atcgcgcgcg ccgaatcggc gatcgggtcc tgctggtcgt cgccctcggt gagcacggtg
     1921 tagaacgcgg tgatcgatcc gccgccttcg cggccgttgc cggcgcgctc gaccagcgtg
     1981 ggcagcttcg cgaacaccga gggcgggtag cccttggttg caggcggctc gccaatggcc
     2041 agcgcgatct cccgctgcgc catcgcatag cgggtcagcg aatccatgat cagcaggacg
     2101 ttcttgccct ggtcgcggaa atgttcggcg atcgcggtcg cataggccgc gccctgcagg
     2161 cgcatcagcg gcggggtgtc ggccggggcg gcgaccacca ccgagcgcgc caggccggcg
     2221 ggtcccagga tttgctcgat gaactccttg acttcgcggc cgcgctcgcc gatcagcccg
     2281 accacgatca cgtcggcgct ggtgtagcgc gccatcatcc cgagcagcac gctcttgccc
     2341 acgccggaac cggcgaacag gcccatgcgc tgcccgcgtc ccacggtcag catgctgttg
     2401 atcgagcgca ccccgacgtc cagcatctgc gagatcgggg cgcgcgcaag cggattggcc
     2461 gggcgcgcat tcagcggcgc cgagtgcgcg gcatggatgg ggcccaggct gtcgagcggg
     2521 cggccggcgc cgtcgaccac gcggccgagc agttcctcgc ccaccggcag gtgccggccg
     2581 cggtcgctcg ggcggcgccg cggatgcgca acccggcccg ggggagggag tgactgcgtg
     2641 acctcgatcg gaaatacgcg cgcgccgggc agcacgccct cgacatcgtt ctgcggcatc
     2701 agcagcagct tgtcattttc gaagccgacc acctcggcct cgatgcgcgc gccgctgggc
     2761 agcggaatgc tgcaggcgct gcccaccgcc agcttgagcc cgacggcttc catcaccagt
     2821 cccgcgaccc gcgtcacccg tccggaaacc tgcagcggtt cggcgatcgc caccagcgag
     2881 cggcaatcgt tcagataggc ctgccagcgg tcgagatggc cgctcatcat tcgagccagt
     2941 cggactgctt gccaagcgct tcggcaatgc gctgccagcg caccggcgcg ccggcctcga
     3001 tctggttgct ggccgtctcg acgcggcagc cgccgcgctc catctgggcg tcctcggcaa
     3061 tgcgccagcc ggccttgtca agttcgtccg tcaagtgcgc gcgcaccagt tccgcgtcgg
     3121 ccggatgcag caccaagagt gcgggctgct gcacgctggg cagatagcgg atcgattcgg
     3181 ccaccaccgg cagcaccagt tcgggacgca ccttgagcgc ggtcttgagc atggccttgg
     3241 ccaggtccag cgacagatgg agcaggtcgt cggcgacctg ctcgttggcc cggttcagat
     3301 cgttcgagaa tgcttcgacc acctggcgca ggcatgcggc ttcgcgcgcc gcttccgcgc
     3361 ggcccgcagc cagcccggct ttgcggccct gctcgaatcc ttcatcgtgg ccctgcttgc
     3421 gcgcctgggc gcgctcctcg gcaagcgcct gctcgtgctg ggcggccagg gcggcgtggt
     3481 cgaccggcgc gggtgcgggc gcgtcgccga acgaggcaag ttcccagcgt tcgtacgcgg
     3541 agagtttttc cttggggatg acggaggagc tcatggggtt cgttctatct ataggggcgg
     3601 ttgtgcaagg taagctgcgt actcccatcc cggccatccc accctcggcc ttgcaaggcc
     3661 tcatgattcg ccgggcgcga agcagtgctt cgcgaaaccc ccggctcatc cccctgagag
     3721 ggggaaggtg agagctgagc tgcgctggag cttaaatgaa ggcatcgtcg cccttggcgc
     3781 cgaggatgat ctgtccttcg tcggccaggc gccgcacgat ctgcaggatt tccttctgct
     3841 gcgcctcgac ctcggacagc cgcaccggcc ccttcgcatc gaggtcctcg cgcatcatct
     3901 cggccgcgcg ctgcgacatg ttcttgaaga tcttctcccg cagttcctgg ctggcgccct
     3961 tcaaggcgat gatcagggat tcggactgca cttcgcgcaa cagcagctgg atgccgcgat
     4021 cgtcgatctc gatgatgttg tcgaacacga acatctcgtc catgatcttc tgcgccatgt
     4081 ccggatcgta gctcttcagg ttttccatca ccgacgcttc cgcgtcgccc gacaggaagt
     4141 tcaggatctc ggccgccgcg cgcacgccgc cgatcggctt cttcttcagc gcctcgttgc
     4201 cggtcaacag cttggtcagc acctcgttga gctcgcgcag cgccgcgggc tgcacgccgt
     4261 cgagcgtggc gatgcgcagt accacgtcgt tgcgcaggcg ttcggtgaag tagccgagca
     4321 cttcgcacgc gtggtaacgc tccaggtgca ccaggatggt cgcgatgatc tgcggatgtt
     4381 cgttgcgcac caggtcggcg acggactggc cgtccatcca cttcaagctt tcgatgccgg
     4441 aggcgtcgcg cgtgccgagg atgcggttga gcagcgtggc cgccttgtcc tcgccgagcg
     4501 cgcgcgtcag cacgctgcgg atgtattcgt ccgagtccat gccgaacgag gtgttcacct
     4561 cggtctcgct gcgaaagtcg tcaagcacct tttccagctg cccgctcgcc accgccttca
     4621 tcgtcgacat ggccgcgcca agcttctgca cttcgcgcgg accgaggtaa cgcatgactt
     4681 ctgccgcttc ctcctcgccc agcgcgagca tcaggatcgc ggctttcatg atcccgtctt
     4741 cactcatttc ccacccatga cttcacgacg ttggcgacca tcttcgggtc cttgcgcgcc
     4801 agttccttcg ccgcatccag gttgtcctgg taggtgcgcg gccgcgaacc catcggcgca
     4861 ggttcatgct cgatctgcac caccgcgtcc tcctcgatct gcggcgcagg cggctgctgc
     4921 ggcggctcga aggtcttcat gatgcggcgg atcagcgggc gcaggaatgc gaagtacagg
     4981 tacagcagaa ggataccgcc aagcatgtac ctgcccgcgg tcatcccgag gtccagcatt
     5041 tccgggcgct tccacagcgg cggctgctcg atgacctccg gctccacccc ggcgaacggg
     5101 ctgttgacca cgttgagcga gtcgccgcgt tcgcggttga agcccatggc ctcgcgcacc
     5161 aggtcggtga tctggttctt ttcgtcgtcg gacagcgcgc gcatgatcgg cttgccctct
     5221 gcatcgaagt cgcgcttgaa attcaccacc acggccaccg acaggcgcct gatgccgccc
     5281 atcggctgct gcacatagcg aacggtcttg tccacctcgt agttgaccgt ggtgtcgcgc
     5341 tgctcgttgg tgggagccgg ggcggcagcc ggggccgctc cgggagcggt ggcggcggcg
     5401 tcggccacga tcggtgcggt cgcaggcgcc ggcggctggt tggtcagcgc tccgggaacg
     5461 ccgaccgcgc cattgggtgt cgtggtacgc gattcgctgc tctgctggct gcggatcgcc
     5521 gaggcgcccg gggcctggtt cggcgtgtag atttccgcgg cctgctcggt atgcgaaaaa
     5581 tcgacgtcgg cggtcgcctc ggcgcgcacg ttgcccggcc cgacgatggg cgtgatgatc
     5641 gattcgatcc ggcgcacgat gctctgctgc agttcctgca cgtacttgag ctgggtcgga
     5701 tcgagcgtat tggcgccttt cttgcccttg tccgacagca ggttgccgtt ctggtcgacc
     5761 accgtgacgt tcttggtcgg cagttccggc acgctgctgg caacgaggtg gacgatcgcg
     5821 ctgacctgct gctcatcaag cgaacgcccc gggtgaaggt tgagcagcac cgaggcggtg
     5881 ggcttctgct ggtcgcgcac gaacaccgaa ttcttcggca gcgccagatg cacgcgcgcc
     5941 gcctggaccg ccgcgaccga ctggatcgag cgcgccagct cgccttccag cgcgcgctgg
     6001 aaattgacct gttcgaggaa ctgcgacacg cccaggcgct ggttctccat cagctcgaag
     6061 ccgacgttgc cgcccttggg caggccctgc gaagcgagcc gcaggcgcac gtcgtgcacc
     6121 tgggaagaag gcaccaggat cgcgccgccg ccgtcggcga acttgtaggg cacgttcatc
     6181 tgctgcagcg cggccacgat cgcaccgccg tcccggtcgg aaaaattcga gaacagcacc
     6241 cggtagtcgg gttccttgct ccacatccag atcaccgaca tcacggcgag cacggcggcc
     6301 acgcccagca tcaggaagaa attgcgtcct cccggtgtct gtgcgaagcc gactagcccg
     6361 gtcggcgcgg tggcggtttc gccggctacc gacatggctg ccctttcgtc ttgaagtcgt
     6421 tattggtcat tcttgggttc gaaggattgc ggcttggtag gggattttct tactgttaag
     6481 caaatctaat cgttgaaaca gaacgtgaat ttccgcactt ctcctgctta gaggcgtatt
     6541 gcgcgagtgc taaggtagcg gcgtgtaaaa aggcggccgc ctgcgtggcg cgccgaaacg
     6601 tggaggatgg aagtgcgatg aaacccgcca tgattgactc aggccggatc gaagcgatga
     6661 tcgcgcaatt gcgcgcggct gcgaatccga ctgcaaatcg gcccattgcc ggggttgcgg
     6721 agaccgctgc cgccgaaaag gtcgcgccga agctggactt tgccgccgca ctgaagaact
     6781 cgctggatca ggtgaacagc gcacagcgca gttccgaggc gctgacccag cgctttgcga
     6841 tgggtgatga cagcgtgagc ctgtcggacg tgatgatcaa ttcccagaag gcgtcgatct
     6901 ccttccatgc caccgtgcag gtgcgcaaca agctggtgtc cgcctatcag gacatcatga
     6961 acatgtccgt gtgagcgggt gtgctaatta cgtgtcgcgt ttgtgcgtga acgtcactgg
     7021 atcccggctt tcgccgggat gacggatgcg gtggtgtgag aggtcgggtc tgcgcgttgc
     7081 gggtgtccgt gaacgtcact ggatcccggc tttcgccagg atgacgcggc agcggaaata
     7141 ggggcgccgg ttcatgcacc gtcactccgg cgaaagccgg agtccagtgt cgcccttcca
     7201 gcttcagccc atccccgtcg ccttggcgtt gcgctcgcgt tcgagcttcg tgatgtagcg
     7261 ctggatcgcc gccagcatcg gcgtcggcag gtcgacgaac atgcagccaa tcttgcgcag
     7321 cgtgcggccg gtcgtgaggg tctggtcgag cgaattgcgc acctggagct tgaccgccag
     7381 cggtgcgccg cccggcagcg ccagacggca ctgctcgtag ctgcggccta tcgtgttgtc
     7441 gagcagcttc ttttcatcga tgatcgcgat gccgccgcag ctgatgtcct tcaccgtcac
     7501 cgaaatcgtc gtgccgtggc tgcccgcatc gtcgggcggg atcgtgatcg tgcatttgag
     7561 cgggctggcg accggcgtgg gcacccggta atgctcacgg cgctggaggc ggaccaggct
     7621 ttgcgggatc ggaatgtaca gcgcgggaag attgtcgtac aggcactcgc gaacctggtt
     7681 cgcgaagaac aggatgcgga tgttgtcgag caccgtctcg aacgacacgt tctcgctgtc
     7741 gaggatgcgc tggttgacga gattggtcgg agcgcggtcg accacgacga tgccgttttc
     7801 ctcatccact tccagaacgg acgtgacgac agcctcggcg ccgccgtcgg cctgcatgct
     7861 gaccagctga ttgcgctcga acatggcgcg cagcagcgcg atgatctccc ggcgcgaatg
     7921 cacctggtag gggctgacgt cctccgtgcc gaatttcgag ttgaatccca ttgaatctgc
     7981 gtgcctgtgg cgaagagttg aaaacgttgc ggctgctatt cgcgcgggag gctgcctggc
     8041 ggtatggcgc cgcccttgat agcgtcgata tggtatagcc aagcgagcag ttccgccacc
     8101 accctgtaca acgccggagg gatgtggcgg tcgaggtcga cctgcatcag cagcgcgaca
     8161 agctcgcgtg attcatgcac gaacacgccg tgttcccgcg cccgcgcgat gatctcgtcg
     8221 gcgatcaggc cgcggccctt ggcgacgacc ttgggcgcga ggtcgccggt ctggtaggcg
     8281 agggcgacgg cattgcgctt cgcatcaggg tgcgccatgg tcccctcgcc gctggacggt
     8341 cagctgttcg agcggcgcgc ccgccgcggc aagcgcatcg gccagccgcg gcgcgaactc
     8401 gcgcagtgcg gcggcgctgg cgtcc
//
LOCUS       DATLGF010000193         3006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_86813,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000193 DATLGF010000000
VERSION     DATLGF010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86813"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VM406_05875"
     CDS             <1..267
                     /locus_tag="VM406_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75525.1"
                     /translation="VFGFSVVRGWARVTPAVPQRARNYIRFFSFSAAADENSRTRAQP
                     SIIPLLACRVAIKKQQPEVSRWIGSKKGPPGKVFVPVCTRSPQA"
     gene            408..2174
                     /locus_tag="VM406_05880"
     CDS             408..2174
                     /locus_tag="VM406_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HVL75526.1"
                     /translation="MLTPLRRLRLRALLFLLCLLAGPAAVAQQSPAYVVAIDGAGPYW
                     ALLSEHLDIVRRQDDTGLTEAEIVRRVGVTPRQIRDLLATEGFFSVDVRHALDRSTTP
                     WTARFELSLGPPAIVESAEIRFQGAIAEGPAADPERMRRLRREWLLVPGEVFQQEQWT
                     AAKNALLRDLLVLDFPAATIADSQARIDPAAASATLMVEIDSGPAFRFGELEIDGLER
                     YSPAIIERANPIRPGERYSQQRLNELQERIQQTGYFRSAFATVEIDPAHAERAPIRVD
                     VNELLRRQLALGGGISSDTGLRVQVRWLDRNFLERDWRLESELRLDRETRLGAADLFL
                     RPFENGWQPSVYAHLARTTSAGETNDTIRTGARLTSPNRRDERAWTVALLADRQRVGD
                     ILANNRQALIGSFIYTRRRMENPLNPRRGYIASVELGVGPRGLVNEDNIARLLVRANA
                     IYPIRENLRFFTRAQVGQVFGGSRLTVPGDLLFRTGGDQTVRGYGFQTLGVPVGGAVV
                     AGNVMAVVSAELVYMFLPNWGGAVFMDAGNAADRWGDLRLARGTGVGVRWSSPLGQIN
                     ADLAYGHETREPRFHFSIGYGF"
     gene            2209..>3006
                     /locus_tag="VM406_05885"
     CDS             2209..>3006
                     /locus_tag="VM406_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75527.1"
                     /translation="MRVVLVALGVLLAVVLALAALAAWLLGTEAGARALLPRVLDRAD
                     AGVTVSGIHGRLLGPLRIDRIVVDRPNERIELADVRLAWRPRALLERRLLHIESLHAA
                     HLLVLGRVEREPEPDTEAPEDLRLPLEVRVDDLRLDGGELRRGPAPLATLGPLALALT
                     YEDEQYRLDLRRFGAAPQFEAGTAQAGVQGRLDLSARHPFPLTGRFQLTSKATVEERA
                     VGASGRLMLNGTLIAMQAALDARINDAPVSGSALLRPFDEQPLGKTEL"
BASE COUNT          457 a         1031 c          981 g          537 t
ORIGIN      
        1 gtgttcggtt tttcagtggt gcgtggatgg gcgcgcgtga cgcccgcggt gccgcagcgg
       61 gcacggaact atatccgctt tttttcattt tcagccgcgg cggacgaaaa ttcccgcacg
      121 agggcgcagc cctcgatcat acctctactt gcgtgcaggg ttgcaatcaa gaaacaacag
      181 ccagaggtct ctagatggat cggttcgaaa aaaggacctc cgggcaaagt ttttgttccc
      241 gtctgcacgc ggtcaccgca agcatgatga tgtacaggtg caagttggtt ttgatctatc
      301 ctaatgcgcc cgcatgctct cccggaaaat aatgtgaagg tgtgcggcag gaacctgacc
      361 atgattccct tttcattccc gctcgaggcc tcgccccaag gagtctcgtg ctgactcctt
      421 tgcgccgact tcggctgcgc gccctgttgt tcctgctctg cctgcttgcc ggcccggcgg
      481 ctgtggcgca gcagtcgccc gcgtatgtgg tcgcgattga cggtgccggc ccctactggg
      541 cgttgctaag cgaacatctc gacatcgtgc gccggcagga cgacaccggc ctgaccgaag
      601 ccgagatcgt gcggcgcgtc ggcgtcacgc cgcgtcagat ccgcgacctg ctcgcgacgg
      661 aaggcttctt ctccgtcgat gtacgtcacg cactggaccg cagcacgacg ccgtggaccg
      721 cgcgcttcga gctgtccctc gggccgccgg ccatcgtcga atcagccgag atccgcttcc
      781 agggcgcaat cgccgagggt ccggccgccg acccggagcg catgcgcagg ctgcgccggg
      841 aatggctgct ggtcccgggc gaggtgttcc agcaggagca atggacggcg gcgaagaacg
      901 ccttgctgcg cgatctgctg gtgctggatt ttccggccgc cacgattgcc gacagccagg
      961 cccgcatcga tcccgccgcc gccagcgcga cgctgatggt ggagatcgat tccggtcccg
     1021 cgtttcgctt cggcgagctc gagatcgacg ggctcgaacg ctattcccct gcgatcatcg
     1081 agcgcgcgaa cccgatccgt cccggcgaac gctacagcca gcagcgcctg aacgagctgc
     1141 aggaacgcat ccagcagacc ggttatttcc gttccgcctt cgccaccgtc gagatcgacc
     1201 cggcgcatgc cgagcgcgcg ccgatacgcg tggatgtcaa cgagttgctt cgccgtcagc
     1261 tcgcactcgg cggcggcatc tcgagcgata ccggcctgcg cgtgcaggtg cgctggctcg
     1321 accgcaactt cctcgaacgc gactggcgcc tcgaatcgga actgcggctg gaccgcgaga
     1381 cgcgcctggg cgcggccgac ctgttcctgc gcccattcga gaacggctgg cagcccagcg
     1441 tgtacgcgca tcttgcccgt accaccagcg ccggcgagac caacgacacg atacgcacgg
     1501 gcgcgcggct gaccagtccg aaccggcgcg acgagcgcgc ctggaccgtc gctctgctgg
     1561 cggatcgcca gcgcgtcggc gacatcctcg caaacaaccg tcaggcgctg atcggctcgt
     1621 tcatctacac gcggcgccgg atggaaaatc cgctcaatcc tcgacgcggc tatatcgcat
     1681 cggtcgaact tggcgtgggg ccgcgcggtc tggtcaatga agacaatatc gcgcgtttgc
     1741 tggtgcgggc gaatgccatt tatccgattc gcgagaatct gcgcttcttc acgcgcgccc
     1801 aggtcggcca ggtgttcggc ggcagtcgcc tgacggttcc cggcgacctg ctgtttcgca
     1861 ccggcggcga ccagaccgtg cgcggctatg gtttccagac ccttggcgta ccggtgggcg
     1921 gcgcggtggt tgccggcaat gtgatggcag tcgtgagcgc cgaactggtc tacatgttct
     1981 tgccgaactg gggtggcgcc gtattcatgg atgcgggcaa cgctgccgat cgctggggtg
     2041 atctgcgcct tgcgcgcggc accggcgtcg gcgtgcgctg gtcgagtccg ctgggccaga
     2101 tcaatgccga cctcgcctac gggcatgaaa cgcgcgaacc acgcttccat ttctccatcg
     2161 gctatggctt ctgaatccaa ggttcgcccg gcccgcgccc ggtccatcat gcgcgtggtc
     2221 ctggtcgcgc tcggcgtcct gctggcggtc gtccttgcgc tcgccgcgct cgccgcctgg
     2281 ctgcttggca ccgaagccgg cgcgcgcgcc ctgctgccgc gcgtgctgga ccgcgccgac
     2341 gcgggcgtga ccgtctcggg cattcacggc cgcctgctcg ggccgctgcg catcgaccgc
     2401 atcgtggttg accggccgaa cgagcgcatc gaactggccg acgtgcgcct ggcctggcgt
     2461 ccgcgtgcgc tgctcgaacg ccggcttctg cacatcgaat ccttgcatgc cgcgcatctg
     2521 ctcgtgcttg gccgcgtgga gcgcgagccc gagcccgaca ccgaggcgcc ggaggatctg
     2581 cgcctgccgc tggaggtgcg cgtcgacgac ctgcgccttg acggcggcga actgcgccga
     2641 gggccagcgc cgctcgccac gcttggaccg ctcgcacttg cgctgactta tgaagacgaa
     2701 caataccgtc tggacctgcg ccgattcggc gccgcgccgc agttcgaagc gggcaccgcc
     2761 caggccggcg tgcagggccg actcgacctg tcggcacgcc acccgttccc gctgaccggc
     2821 cgctttcagc tcaccagcaa ggccaccgtg gaggagcgtg ccgtcggcgc ctccgggcgg
     2881 ctgatgctga acggcacctt gattgcgatg caggctgcgc tcgatgcgcg catcaatgac
     2941 gcacccgtca gcggcagcgc gctgctgcgg ccgttcgacg aacagccgct cggcaagacc
     3001 gaactg
//
LOCUS       DATLGF010000194         4755 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_4112,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000194 DATLGF010000000
VERSION     DATLGF010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4112"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..517
                     /locus_tag="VM406_05890"
     CDS             137..517
                     /locus_tag="VM406_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75528.1"
                     /translation="MTIVAFAPAAVAEPGENAAAVFAKPVVLRGTLGDASIQMQLRPK
                     EEFEGGVEGQYFAFGQSGHVLVAGEVDGDEVFMEESVNGRDVSGDWAGRFHDGVFSGE
                     WQGMQGEVRPFRLEILRINKEKQK"
     gene            529..1710
                     /locus_tag="VM406_05895"
     CDS             529..1710
                     /locus_tag="VM406_05895"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020693834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HVL75529.1"
                     /translation="MSVRKSVQTALIHSDYTPPPGFAAFPTPIHHASTVLFKDVASMR
                     ARDWKDKNCYTYGLHGTPTSFTLEARLAAIEGGAHCMLAPSGLAAISMVNFGLLRSGD
                     DLLLPDNVYGPSRELGRWLEQDYGITARVYDPMIGAGIAELIRPNTRLVWTEAPGSVT
                     MEVPDIPAICAAVAARRSGGQQIWTAIDNTWSAGIGFRPFEHGIDISMQALTKYQSGG
                     SDVLMGAVITQDEALMQRIKMAHMRIGFGVGMNDVYLVLRGLESMKLRFDAHDAGARK
                     VAAWLKTRPEIARVLHPAFEDCPGHDAWKRDFNGAGGLFSVLFDPGYEEARIDRFVEA
                     LQLFGIGFSWGGSHSLCVPYRVKGMRTRWKDEGVLVRFNIGLEAPDDLIADIGQALDK
                     L"
     gene            complement(1729..2088)
                     /locus_tag="VM406_05900"
     CDS             complement(1729..2088)
                     /locus_tag="VM406_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75530.1"
                     /translation="MAQELIFDPQTERAKTLAWWLYIFHGIGFVFSLGVFSWIPLIIN
                     YIRRPDAAGTFVYSHHSWQIRSFWWYIAWMIIGGILFITLIGIPLAWLVWTGAWIWQA
                     YRLIRGILDLNDNKPMP"
     gene            complement(2090..2932)
                     /gene="serB"
                     /locus_tag="VM406_05905"
     CDS             complement(2090..2932)
                     /gene="serB"
                     /locus_tag="VM406_05905"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HVL75531.1"
                     /translation="MNLILQGLTPTPADIDRIAALAGARSIVPIGPAAFRCEDVAPTE
                     AQKKEVEQAAYDARLDCTFIAPGRRLSDFGLVTMDMDSTLITIECIDEIADMQGLKPQ
                     VAEITEAAMRGELDFRESLALRVALLKGLDAGALQRVYDDRLRLSPGAERMLEAVKAA
                     GLKTLLVSGGFTFFTDRIKTRLGLDFTHSNELEVYEGKLTGRVVGTIVDADEKKATVE
                     RVCREIGISPRQAIVMGDGANDLKMMAIAGMSVAFRAKPIVRSQADVALNFVGLDGIL
                     PLFE"
     gene            complement(3063..>4755)
                     /locus_tag="VM406_05910"
     CDS             complement(3063..>4755)
                     /locus_tag="VM406_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HVL75532.1"
                     /translation="HVFEYSARDYEAFAESFGFEETPDQRAAINAVIQDMTSGRPMDR
                     LICGDVGFGKTEVALRAAFVAVMGGKQVAILAPTTLLAEQHAQTFADRFADWPVRIAE
                     LSRFRTGKEVTQAVKGIADGSIDIVIGTHKLLSNEVKFSRLGLVIIDEEHRFGVRQKE
                     ALKALRAEVDVLTLTATPIPRTLGMALEGLRDFSVIATAPQKRLAIKTFVRREDESVI
                     REACLRELKRGGQVYFLHNEVETIQNRKAMLEALLPEARIGVAHGQMHERDLERVMRD
                     FVSQRFNILLCTTIIETGIDVPTANTIVMHRADKFGLAQLHQLRGRVGRSHHQAYAYL
                     LVHDVQGLTKQAQRRLEAIQQMEELGSGFYLAMHDLEIRGAGEVLGENQSGEMVEVGF
                     QLYSDMLNEAVRALKNGKEPDLAAPLSTTTEINLHVPALLPQDYCGDVHERLSLYKRL
                     ANCRSAEDIDALQEELIDRFGKLPESAKALIETHRLRIAAMPVGIVKIDAHAESAVLQ
                     FQPNPPIDAMRIIELVQKNRNIKLSGQDKLRVTANMPDLAARVAQVKSTIRSLVG"
BASE COUNT          819 a         1452 c         1588 g          896 t
ORIGIN      
        1 acgtgaagcc gccttatgag ccgcatcgca agctgaaggt cggcgagttc gtcgaggtga
       61 cgatcaccgc cgccgacgcg cacgacctgt gggccgaggc atgaggcggc gtctcgccgc
      121 actggcgtgc gcggcgttga cgatcgtcgc cttcgcgccg gcggcggtgg ccgaaccggg
      181 tgagaacgca gcggccgtgt ttgccaagcc cgtggtgctg cgtggcacgc ttggcgatgc
      241 aagcattcag atgcagctgc gcccgaaaga agaattcgaa ggcggcgttg aggggcagta
      301 ttttgcgttc ggacagtccg gacatgtgct cgtggccggt gaggtcgacg gcgatgaagt
      361 gttcatggaa gaatccgtca acggcagaga cgtctccggc gactgggccg gccggttcca
      421 cgacggcgtg ttcagcggcg agtggcaggg tatgcaggga gaggtccggc cgttccggct
      481 ggaaatcctg cgcatcaaca aggaaaaaca gaaataaggg agacaggcgt gagcgttcgc
      541 aaatccgtgc agactgcatt gatccattcc gattacacgc cgccgcccgg cttcgcggcc
      601 ttccctaccc ctatccatca tgcgtccacg gtcctgttca aggacgtggc ctcgatgcgc
      661 gcacgcgact ggaaggacaa gaactgctac acctacggcc tgcatggcac gccgacgagc
      721 ttcacgctgg aagcgcggct tgccgcgatc gaaggcggcg cccactgcat gcttgcgccg
      781 agcggcctgg cggcgatctc gatggtgaac ttcgggctgc tcaggagtgg tgacgacctg
      841 ctgctgccgg acaacgtcta cggccccagc cgtgaactgg gacgctggct ggagcaggac
      901 tacggcatca ccgcgcgggt ctacgatccg atgatcggcg caggcatcgc cgagctgatc
      961 cgtccgaata cccggctggt gtggaccgag gcacccggtt ccgtgaccat ggaagtgccc
     1021 gatatcccgg cgatctgcgc cgcggttgcg gcgcggcgca gcgggggaca acagatctgg
     1081 accgccatcg acaatacctg gtcggcggga atcggctttc gcccgttcga gcatgggatt
     1141 gacatctcga tgcaggcgct cacgaagtac cagtccggcg gttcggatgt gctgatgggc
     1201 gccgtcatta cccaggacga ggcactgatg cagcggatca agatggcgca catgcgcatc
     1261 ggcttcggcg tcggcatgaa cgacgtctac ctggtgctgc gcgggctgga atcgatgaag
     1321 ctgcgcttcg atgcgcatga cgccggcgca cgcaaggtgg cggcatggct gaagacgcgg
     1381 ccggaaatcg cccgcgtgct gcatccggcg ttcgaggact gtccgggcca cgatgcatgg
     1441 aagcgcgact tcaacggcgc cggtggcctg ttctcggtgc tgttcgatcc gggttacgag
     1501 gaagcgcgga tcgaccgctt cgtcgaggcg ctgcagctgt tcggcatcgg tttcagctgg
     1561 ggcggctccc atagcctgtg cgtgccgtac cgggtaaagg gcatgcgcac gcgctggaag
     1621 gacgaaggcg tgctggtgcg cttcaacatc ggcctcgagg ctcccgatga cttgatcgcg
     1681 gacatcggtc aggcgctcga caagctctga gcgctcgtcc cggcagcctc agggcattgg
     1741 cttgttgtcg ttcagatcga gaatgccgcg aatcagacgg taggcctgcc agatccatgc
     1801 gccggtccag accagccagg ccagtgggat gccgatcagc gtgatgaaca ggatgccgcc
     1861 gatgatcatc cacgcgatgt accaccagaa cgagcggatc tgccagctgt ggtgcgagta
     1921 gacgaaggtg ccggccgcgt cgggacggcg gatgtagttg atgatcagcg ggatccagga
     1981 aaagaccccg agcgagaaca cgaagccgat gccgtgaaag atgtacagcc accacgccag
     2041 ggtcttggcg cgctcggtct gcgggtcgaa tatgagttcc tgggccattt cactcgaaaa
     2101 gcggcagtat tccgtccaag cctacaaaat tcagcgccac atcggcttgt gatcggacaa
     2161 tcggtttcgc ccgaaaagca accgacatgc ctgcgatcgc catcatcttc aggtcgtttg
     2221 cgccgtcccc catcacgatg gcctgccgcg gggaaattcc gatctcccga cacacgcgct
     2281 cgaccgtggc tttcttttca tccgcatcga cgatggtgcc gaccacccgc ccggtaagct
     2341 tgccctcata gacctccagc tcgttcgagt gggtgaagtc gagccccagg cgcgtcttga
     2401 tccggtcggt gaagaaggtg aagccacccg acaccagcag ggttttcagg ccggccgcct
     2461 tgacagcttc gagcatgcgt tcggcgccgg gcgacaggcg cagccggtcg tcgtacacgc
     2521 gctgcagcgc gccggcgtcc agacccttga gcagggccac gcgcagggcc aggctttcac
     2581 ggaaatccag ctcgccgcgc atcgccgcct cggtgatctc ggcgacctgc ggcttcaatc
     2641 cctgcatgtc cgcgatctcg tcgatgcatt cgatcgtgat cagcgtcgaa tccatgtcca
     2701 tcgtgaccag tccgaagtcg gagagcctgc ggccgggcgc gatgaaggtg cagtcgagcc
     2761 gggcgtcgta ggcggcttgt tcgacttcct tcttctgggc ttcggtgggg gcgacgtcct
     2821 cgcagcggaa ggcagcgggg ccgattggga cgattgagcg ggcgccggcg agggcggcga
     2881 tgcggtcgat gtcggcgggc gtaggggtga ggccttggag gatgaggttc atgcgggtgg
     2941 tgggttgggt gattcgtttc ttggtgcgtg taccgattct taagttgccg ccgtacggtt
     3001 cccccattta acggggaggg atagggtggg ggtacccgag tttcacgtaa cgtcttttcc
     3061 tctcatccca ccagcgaccg tatcgtcgat ttcacctgcg cgaccctggc cgccagatcc
     3121 ggcatgttcg cagtcacgcg cagcttgtcc tgtccgctca gcttgatgtt gcggttcttc
     3181 tggaccagct cgatgatgcg catcgcatcg atcggcggat tcggctggaa ctgcagcacc
     3241 gccgattcgg cgtgggcgtc gatcttgaca atgcccaccg gcattgcggc gatgcgcagg
     3301 cgatgggttt cgatcagcgc ctttgcggac tcgggcagct tcccgaagcg gtcgatcagc
     3361 tcttcctgca aggcgtcgat gtcctcggcg gacctgcagt tggccagccg cttgtacagc
     3421 gacaggcgct catgcacgtc gccgcaatag tcctgcggca gcagggccgg gacatgcaaa
     3481 ttgatttcgg tggtggtcga cagcggcgcc gccagatcgg gctccttgcc gttcttcagt
     3541 gcgcgcaccg cttcgttgag catgtccgaa tacagctgga agccgacttc caccatctcg
     3601 cccgactggt tctcgccgag cacttcgccg gcgccgcgga tctccaggtc gtgcattgcc
     3661 aggtagaagc cgctgccgag ctcctccatc tgctggatcg cctccaggcg gcgctgcgcc
     3721 tgcttggtca ggccttgcac gtcgtgcacg agcaggtagg cgtaggcctg gtgatgcgag
     3781 cgcccgacgc ggccgcgcag ctggtgcagc tgggccaggc cgaacttgtc ggcgcggtgc
     3841 atgacgatgg tgttcgccgt cggcacgtcg atgccagtct cgatgatggt cgtgcaaagc
     3901 aggatgttga agcgctgcga gacgaaatcg cgcatgactc tttccaggtc gcgctcgtgc
     3961 atctggccgt gggccacgcc gatgcgcgct tccggcagca gcgcttcgag catcgccttg
     4021 cggttctgga tggtttcgac ctcgttgtgc aggaaataga cctggccgcc gcgcttcaat
     4081 tcgcgcaggc aggcttcgcg gatcacggac tcgtcctcgc ggcgcacgaa ggtcttgatc
     4141 gccagccgtt tctgcggggc ggtggcgatc acggagaaat cacgcaggcc ttcgagcgcc
     4201 atgcctagcg tgcgcggaat cggcgtggct gtcagcgtca gcacatcgac ctcggcgcgc
     4261 aaggccttca gcgcttcctt ctggcgcaca ccgaagcggt gttcctcgtc gatgatgacc
     4321 agcccgagcc gggaaaattt cacttcattc gacagcagct tgtgggtgcc gatcacgatg
     4381 tcgattgatc cgtcggcgat gcccttgacc gcctgggtga cttcctttcc ggtacgaaag
     4441 cgcgacagtt cggcgatgcg caccggccag tcagcgaagc ggtcggcgaa ggtctgcgca
     4501 tgctgttcgg ccagcagtgt ggtcggggcg aggatggcga cctgcttgcc gcccatgacc
     4561 gcgacgaagg ccgcacgcag cgcaacttcc gtcttgccga aaccgacgtc gccgcagatc
     4621 agccggtcca tcgggcggcc ggaagtcatg tcctggatca ccgcattgat cgcggcgcgc
     4681 tggtcgggtg tttcctcgaa accgaagctc tccgcgaagg cttcgtagtc gcgcgccgag
     4741 tactcgaaca catgg
//
LOCUS       DATLGF010000195         2549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_86889,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000195 DATLGF010000000
VERSION     DATLGF010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86889"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="VM406_05915"
     CDS             complement(<1..166)
                     /locus_tag="VM406_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL75533.1"
                     /translation="MNVTLRQLRAFIALARTGSFTLAADSLHVTQSALSGLIKELEQA
                     VGVQLVDRTTR"
     gene            323..865
                     /locus_tag="VM406_05920"
     CDS             323..865
                     /locus_tag="VM406_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVL75534.1"
                     /translation="MKIQQIHHVAYRCKDAKETVEWYVKHLNMNFVLAIAENEVPSTK
                     APDPYMHVFLDAGNGNILAFFELPSQKPMGRDENTPTWVQHLALKVGSMEELIATKER
                     LQAAGIDVVGVTDHTIFKSIYFFDPNGHRIELACDTATPEMMQKLDEVKWDMLNEWAQ
                     TKRAPNHAAWMHDGSFAAKQ"
     gene            962..1984
                     /locus_tag="VM406_05925"
     CDS             962..1984
                     /locus_tag="VM406_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HVL75535.1"
                     /translation="MKLATLKAGGRDGTLVVVNRDLTLCQAVPEIARTLQAALDDWER
                     CAPQLQDVYAALNNGAARHAQPFDEKACHSPLPRAYQWADGSAYLNHVELVRKARNSE
                     VPASFWTDPLMYQGGSDSFVGPRDPVYALSEDWGIDLEAEVAVVTGDVPMGATPEQCA
                     QAIRLVMLVNDVSLRNLIPNELAKGFGFFQSKPASAFSPVAVTPDELGDAWKDAKVHL
                     PLLVEINDKPFGRPNAGEDMTFSFGQLVAHVAKTREAEAGSIIGSGTVSNKQGNLHGS
                     SIENGGVGYCCLAEVRMYETIEGGAPKTSFLKFGDKVKIEMRDADGNNIFGTIEQTVT
                     RYPGAR"
     gene            2076..>2549
                     /gene="maiA"
                     /locus_tag="VM406_05930"
     CDS             2076..>2549
                     /gene="maiA"
                     /locus_tag="VM406_05930"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HVL75536.1"
                     /translation="MLKLHTYFRSSAAYRVRIALNLKGLDYEQVSVHLLRDGGEQFKD
                     AYRALNPNALVPTLEDGDVVLNQSLAIIEYLDEKFPGTARLLPAAPAERARVRALALD
                     IACDIHPLNNLRILKYLKKELGASEEAKNAWYRHWVQEGLAALETQLARSPATGRY"
BASE COUNT          526 a          872 c          771 g          380 t
ORIGIN      
        1 ggcgcgtggt gcggtcgacc agctgcacgc cgaccgcctg ctccagttcc ttgatcaaac
       61 cggagagcgc cgactgcgtg acgtgcaaac tgtcagctgc aagcgtgaag ctgccggtgc
      121 gggccagcgc gatgaaggct ctcaactgcc gcagcgtgac gttcataagg aaacctcata
      181 aatgcattca aaaaatctgt ttgaatgata aacgcgattg gactttaatg gctgcatcga
      241 catcctccgt gcgcaagcaa cggaggtatt cgcggcgccg gccggcgtcg cgcagtaccc
      301 gcaacccatt tgggagacac cgatgaaaat ccagcagatc caccacgttg cctaccgctg
      361 caaggatgcc aaggagacgg tcgagtggta tgtgaagcac ctgaacatga acttcgtgct
      421 ggcgatcgcc gagaacgaag tgccctccac gaaggcgccg gacccgtaca tgcacgtgtt
      481 cctggacgca ggcaacggca acatcctcgc cttcttcgaa ctgccgagcc agaagccgat
      541 gggccgcgac gagaacaccc cgacctgggt gcagcacctg gcgctgaagg tcggctcgat
      601 ggaagaactg atcgcaacca aggagcgcct gcaggcggcc ggcatcgacg tggtcggcgt
      661 gactgatcac acgatcttca agtcgatcta cttcttcgac ccgaacggcc atcgcatcga
      721 actggcttgc gacaccgcga cccccgaaat gatgcagaag ctcgacgagg tgaagtggga
      781 catgctcaac gagtgggcgc agaccaagcg cgcgcccaac catgcggcgt ggatgcacga
      841 cggaagtttt gcggcgaagc agtaaggctc acggccggat tgcccgaacg ggcagtccgg
      901 cgttcggttc gcaccacagc actgcagcac tccacgacca ccacttgcat actcaacaac
      961 catgaaactt gccactctga aagccggtgg acgcgacggc accctggtcg tcgtcaaccg
     1021 cgacctcacc ctctgccagg cggtgcccga aatcgccagg acgctgcagg ccgcgctgga
     1081 cgattgggag cgctgcgcac cccagctgca ggacgtgtat gcggcgctca acaacggcgc
     1141 agcccgccat gcccagccct tcgacgagaa ggcctgccac tcgccgctgc cgcgcgccta
     1201 ccagtgggcc gacggctcgg cctacctgaa ccacgtggaa ctggtgcgca aggcgcgcaa
     1261 cagcgaagta cctgcctcgt tctggaccga tccgctgatg taccagggcg gctccgactc
     1321 cttcgtcggc ccgcgcgatc cggtgtatgc gctgtccgaa gactggggca tcgacctgga
     1381 agccgaagtg gcggtggtca ccggcgacgt gcctatgggc gccacgcccg agcaatgcgc
     1441 acaggcgatc cgcctggtga tgctggtcaa tgacgtgtcg ctgcgcaacc tgatcccgaa
     1501 cgaactggcc aagggcttcg gcttcttcca gtccaagccc gccagcgcct tctcgccggt
     1561 ggccgtgacc ccggacgagc tcggcgacgc ctggaaggat gccaaggtcc acctgccgct
     1621 gctggtggag atcaacgaca agcccttcgg ccgcccgaac gccggcgaag acatgacctt
     1681 cagcttcggc cagctggtcg cccacgtggc caagacgcgc gaagccgaag ccggctccat
     1741 catcggctcc ggcaccgtat cgaacaagca gggcaacctg cacggctcct cgatcgagaa
     1801 cggcggcgtc ggctactgct gcctggccga ggtgcgcatg tatgaaacga tcgaaggcgg
     1861 cgcgcccaag acctcgttcc tgaagttcgg cgacaaggtc aagatcgaaa tgcgcgacgc
     1921 cgacggcaac aacatcttcg gcaccatcga gcagacagtg acgcgctacc cgggcgcgcg
     1981 ctgatgcgcc cggcggcagc cccgtgctgc cgcccgctga ccggccgggc cgcgctgcgc
     2041 ggcccaggcc caagaccgca aaacagatcc ccaccatgct aaagctccat acctatttcc
     2101 gcagctcggc cgcctaccgg gtgcgtatcg cgctcaacct gaaaggcctg gactacgaac
     2161 aggtttccgt gcacctgctg cgcgacggcg gcgagcagtt caaggacgcc taccgcgcgc
     2221 tcaatccgaa cgcgctggtg cccacgctgg aagacggcga tgtcgtcctg aaccagtcgc
     2281 tcgcgatcat cgaatacctg gacgagaaat tccccggcac ggcccgcctg ctgcccgccg
     2341 cgcccgccga acgcgcccgc gtgcgcgcgc tggcgctgga cattgcctgc gacatccatc
     2401 cgctcaacaa cctgcgcatc ctgaagtacc tgaaaaagga actcggcgcg agcgaagaag
     2461 caaagaacgc gtggtaccgc cactgggtgc aggaaggcct cgccgcgctc gaaacccagc
     2521 tcgcccgcag ccccgccacc ggccgctac
//
LOCUS       DATLGF010000196         2426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_86890,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000196 DATLGF010000000
VERSION     DATLGF010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86890"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VM406_05935"
     CDS             <1..538
                     /locus_tag="VM406_05935"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HVL75537.1"
                     /translation="AKALLAHLPPFVASVGLLVNTSLDEAKAVVARAPVSMLQFHGDE
                     SLEQCCAIAEAVQRPFMRALGIGSAAAAADLLECEQRFRAASPLFSGFVLDAVVEGYG
                     GGGKVFDWSLIPKELAPRAVLSGGLNAQNAAEAIREVRPYALDVSSGVEQSRGVKDAA
                     LIRAFIAAAREADASLRE"
     gene            551..1819
                     /gene="trpB"
                     /locus_tag="VM406_05940"
     CDS             551..1819
                     /gene="trpB"
                     /locus_tag="VM406_05940"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HVL75538.1"
                     /translation="MKEITPSGGYGERTPVAGTTIVQSASYNLPDARGHFGPYGGSFV
                     SETLTHALAELKNGYAHFRTDPDFLAEFERELKHFVGRPSPVYHARRWSEMQGGAQIY
                     FKREDLNHTGAHKINNVIGQALLAKRMGKPRVIAETGAGQHGVATATICARFGLECVV
                     YMGAEDVKRQLQNVYRMELLGAKVVPVESGSKTLKDALNEAMRDWVTNVENTFYIIGT
                     VAGPHPYPMMVRDFQSVIGRECIEQMPEQAGRQPDYVVACIGGGSNAMGIFYPYIDHR
                     DVQLIGVEAAGEGLETGRHSASLTAGTPGVLHGNRTYLLQDADGQIIETHSVSAGLDY
                     PGVGPEHAWLKDAKRAQYVTITDEEAVEAFHTCCRIEGIIPALESSHALAYAAKLAAT
                     LPKDKIILANLSGRGDKDMHTVAERMKRKI"
     gene            1890..>2426
                     /gene="trpA"
                     /locus_tag="VM406_05945"
     CDS             1890..>2426
                     /gene="trpA"
                     /locus_tag="VM406_05945"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HVL75539.1"
                     /translation="MSRIQSTLSALAARNRKGLIPFITAGDPDPDLAVALMHALVAGG
                     ADIIELGVPFSDPMADGPVIQRASERALAKGVGLRRVLEFVREFRSSNQSTPVVLMGY
                     ANPIEHMGVAAFIAAAKEAGVDGVLVVDYPPEECEEFAAALKENGLDPIFLLAPTSTD
                     ERIAQVGKVASGYVYYVSL"
BASE COUNT          438 a          783 c          786 g          419 t
ORIGIN      
        1 cgccaaggcc ctgctcgcgc atcttccgcc gtttgttgcg tccgtcggcc tgctggtgaa
       61 cacgagcctc gacgaggcga aggctgtcgt cgcgcgggcg ccggtgtcga tgttgcaatt
      121 tcatggcgac gagagcctgg agcagtgctg cgcgatcgcc gaagccgtgc agcggccttt
      181 catgcgcgcc ctcggcattg gttcggctgc cgccgcagcc gatttgctag aatgcgaaca
      241 gcgatttcgt gccgccagtc ccttgttttc cggcttcgtc ctcgatgcgg tcgtcgaggg
      301 ctacggcggc ggtggaaagg ttttcgattg gtctctcatc ccaaaagaac tcgcgcctcg
      361 ggccgttttg agtggtggct tgaacgcgca aaacgcggct gaagcgatcc gcgaggtccg
      421 tccctatgca ctcgacgtca gcagcggcgt cgaacaatcc aggggcgtca aggatgccgc
      481 gctcatccgt gcgttcatcg cagcggcgcg cgaagccgac gcctccctcc gcgaatagaa
      541 gagagttgcc atgaaagaga taaccccatc cggcggctac ggcgagcgta cgcctgtcgc
      601 cggcaccacg atcgtccagt ccgcgagcta caacctgccc gatgcgcgcg gacatttcgg
      661 cccctacggc ggcagtttcg tctccgagac cctcacccat gcgctggccg agctgaagaa
      721 cggctatgcg catttccgta ccgatccgga tttcctggcc gagttcgagc gtgagctcaa
      781 gcacttcgtc ggacgtccga gccccgtcta tcacgcccgc cgctggtctg agatgcaggg
      841 cggcgcccag atctatttca agcgcgaaga cctgaatcac accggcgcgc acaagatcaa
      901 caacgtgatc ggtcaggcct tgctcgccaa gcggatgggc aagccgcgcg tgatcgcgga
      961 gaccggggcg ggacagcacg gcgtggccac cgccacgatc tgcgcgcgct tcgggctgga
     1021 atgcgtcgtc tacatgggcg ccgaggacgt gaagcgccag ctgcagaacg tgtaccggat
     1081 ggaactgctg ggcgcgaagg tggtgccggt ggagtcgggc tcgaagacgc tcaaggatgc
     1141 gctcaacgaa gcgatgcgcg actgggtgac caatgtcgag aacacgttct acatcatcgg
     1201 caccgttgcc ggtccgcacc cttacccgat gatggtgcgt gacttccagt cggtgatcgg
     1261 gcgcgaatgc atcgagcaga tgccggagca ggccgggcgc cagccggatt atgtcgtggc
     1321 ctgcatcggc ggcggctcca atgcgatggg gattttctat ccgtacatcg atcaccgcga
     1381 cgtgcagctg atcggcgtcg aggcagccgg ggaagggctg gagaccggcc gtcattcggc
     1441 gtcgctgacc gccggcacgc ctggcgtgct gcatgggaac cggacgtatc tgctgcagga
     1501 tgccgatggt cagatcatcg agacccactc ggtctcggcc gggctcgact acccgggcgt
     1561 cgggccggag catgcatggc tgaaggatgc caagcgtgcc cagtacgtga cgatcactga
     1621 cgaggaggcg gtggaagcct tccacacctg ctgccgcatc gaaggcatca tccccgcgct
     1681 ggagtcgagc cacgcgctgg cctacgcggc caagctcgcg gcaacgctgc cgaaggacaa
     1741 gatcatcctc gccaacctct ccgggcgggg ggacaaggac atgcatacgg tggccgagcg
     1801 catgaaacgg aagatctgag cgcccgcgcc cggtcacgcc agggagccgg ccacgcgccg
     1861 gaacgacaca acaaccacta caccgcatca tgtccagaat ccaatccaca ctgtccgccc
     1921 ttgccgcccg caaccggaag ggcctgattc ccttcatcac cgcgggcgat cccgatccgg
     1981 atctcgcggt tgccctgatg cacgcgctcg tcgcgggcgg cgccgacatc atcgaactcg
     2041 gcgttccgtt ctcagatcca atggccgacg gtccggtgat ccagcgggcg tctgaaagag
     2101 cgcttgcaaa gggcgtcggc ttgcgtcgcg ttctcgagtt cgtgcgcgaa ttccgctcga
     2161 gcaatcagtc cacccccgtc gtcctgatgg ggtatgccaa tccaatcgag cacatgggcg
     2221 tggcggcctt cattgcggca gcgaaggagg ctggcgtcga tggcgtgctg gtggtcgact
     2281 acccgcccga ggaatgcgag gagttcgcgg ccgcactgaa ggaaaacggc ctcgatccta
     2341 tcttcctgct cgcgccgacc tcgaccgacg agcggatcgc acaggtcggc aaggtcgcca
     2401 gcggctacgt ctactacgtg tcgctc
//
LOCUS       DATLGF010000197         6836 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_55131,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000197 DATLGF010000000
VERSION     DATLGF010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6836
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55131"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1903)
                     /locus_tag="VM406_05950"
     CDS             complement(<1..1903)
                     /locus_tag="VM406_05950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HVL75540.1"
                     /translation="MSQLDQGFLLTRHWRDTPGGTEVEFWLATDAGPRQVRVPAQPSV
                     AFIPAVQREQAQAALRGERGCELRELALRDFQRRPVLGLYCAQYRQLLKLEKRLRESG
                     VDVFEADVRPPERYLMERFITAPVSFSARAAGDGSGLLLDAQMKPAPGYRPRLKLVSL
                     DIETSARGDLYSIALEGCGRRDVLMLGPRNGGGDALDFDLVYCDSPAQLLERLNAWLA
                     RHDPDAIIGWNLVQFDLRLLQKHAQRLGIALRFGRDGSEMEWREHSRGQHYFAGIAGR
                     LVIDGIEALKSATWNFASFSLEHVAQTLLGEGKAIDNPYDRMAEIERRFREDKPALAR
                     YNLKDCELVTRIFHHTGLMDFLLERASVTGLAADRSGGSVAAFEHLYMPLMHRQGYVA
                     PNLGDVAGENSPGGFVMDSRSGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGGASAD
                     ENAVVPGFRGARFSRDKHCLPAIVTQVWQERETAKRDGNHALSQALKIIMNAFYGVLG
                     SSGCRFFDPRLASSITLRGQEIMRQTRDLIEAQGYPVIYGDTDSTFVWLRHAHSEQQA
                     MEVARTLVEHVNQWWARHLHEAFGLASALELEFDTHYRRFLMPTIRGSDEGSKKRYAG
                     LVVQPDGSEE"
     gene            2192..3199
                     /locus_tag="VM406_05955"
     CDS             2192..3199
                     /locus_tag="VM406_05955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL75541.1"
                     /translation="MQFEACPACLGENIKTIHRIATRDLETLYGREGLDVSRYFGATR
                     EIRLNKCLACDLGFFSPHCAGDDLFYEQLQKFDWYYQDDKPEYDYARKLVGDAQRVLE
                     VGCGKGAFFSWLPESVEYTGLEFNDEAIRKARSAGLNVFKQPIEEHAKLEAGKYDVVC
                     SFQVLEHIPAPRGFIRACVQALKPGGKLIFAVPAEDGFLGVAANAYLNMPPHHVLRWS
                     DRALRHLAFREGLSVIELWHEPVAEFHREWHQETLARHYLVQLGLGQQRLVDRTLTGR
                     VFSRLLRSGGIRDYFAAKAAKRFAPATHGHTVVLVASKPVWSEVHAVQRKASPDTVVA
                     Q"
     gene            3434..3979
                     /locus_tag="VM406_05960"
     CDS             3434..3979
                     /locus_tag="VM406_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HVL75542.1"
                     /translation="MNRAGVAQGNGRAQQQAVLATPTDLQDAQTRDIAGAMNRVLADV
                     FALYLKTKNFHWHMSGPHFRDYHEMLDEQSDQLFAMTDPIAERVRKIGKTTLRSIGEI
                     SRLQSIRDNDADFVSPQQMLAELCGDNKTLATRLRETHEVCDGGRDIASASLIETWIE
                     ETERRTWYLFEASRAPDKTGH"
     gene            complement(4041..4721)
                     /locus_tag="VM406_05965"
     CDS             complement(4041..4721)
                     /locus_tag="VM406_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL75543.1"
                     /translation="MLGLADSEVLDVLLVDDHPVVRVGFATLLGQLHKTMCFYEAEDA
                     VSALALATARRPRIALVDLSLAGVTSLGLVKDLHEAVPGMAILVTSMHDERLYAERAL
                     RAGARGYMMKHNASSSVVKAVETVCAGRIWLSDAMQTIVLGRLSPGTGETTSNALATL
                     SDREIEVFQLIGMGQKKTEIAERLSLSPHTVETYRTQIKRKLGIASGAELYRMAFLHF
                     SEQNPGAR"
     gene            complement(4715..6439)
                     /locus_tag="VM406_05970"
     CDS             complement(4715..6439)
                     /locus_tag="VM406_05970"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL75544.1"
                     /translation="MRISAQLPRIILVVGTIVTLWGAWSVQRQIDHEAESAFASQVKS
                     FETTLIRRLRSYEDILFGVAGLFRSRDGVTMEEFEAYATSLNAAQRYPALAVINFARY
                     GTRAQRDAFVRDFERHTEGRKRLPLAIPESQPEFMVITHLYPSKSPGLGLDIVQNARQ
                     RARDPSAPPILVTPAMFPVNAVYSSAIPFTPPGNAPPGIAARLGVYRPDPSGNEVLIG
                     SAGIGFTVPALFAEALPPQWAGQLRYRVDNLGRTSGARHEQPVLVHRSSPAPVDDPPR
                     GMISASFAFDFGGADLQGTVQAERSSLVNPWYETLPPAVLLFGALLTVLLSVLSRRTL
                     HANGRLETAVAARTSELRAELERVRELEREVARIADDEQRRIGNELHDDLGQRLTGMS
                     LAAAALAADMQAAAPHLHPRAKALERSCSECISEVRKLAHGLLPVAEGPSGLREALAQ
                     LAVDVQAMTGVACSFDFDDPVDIDDSADAAHLYRIAQEAVSNAIRHGNAARIDLRLDY
                     MEGKVMLSIQDDGIGFDCDADKTNTGLWALEGAGMRVMRYRASVIGYRLAVESKPGTG
                     TCIRVTQC"
     gene            6592..>6836
                     /locus_tag="VM406_05975"
     CDS             6592..>6836
                     /locus_tag="VM406_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75545.1"
                     /translation="MTEQHPVSIGITAPGHLMTTTFIEPIDATLVCAHPPSAPALASK
                     RLYRLKAGERIGPYSFREIAQYIGATRFAPDEMIWHER"
BASE COUNT         1194 a         2208 c         2227 g         1207 t
ORIGIN      
        1 cctcctcgct gccgtccggt tgcacgacca ggccggcata gcgcttcttg ctgccctcgt
       61 cggagccgcg gatggtcggc atcaggaagc gccggtagtg tgtgtcgaac tccagttcca
      121 gcgcgctcgc caggccgaac gcctcgtgca ggtggcgtgc ccaccactgg ttgacgtgct
      181 cgaccagcgt gcgtgcgact tccatcgcct gttgttccga atgcgcgtgc cgcagccaga
      241 cgaaggtgga gtcggtgtcg ccgtagatga ccggatagcc ttgcgcttcg atcaggtcgc
      301 gcgtctgccg catgatctcc tgcccgcgca gtgtgatcga cgacgccagc cgcggatcga
      361 agaagcggca gccgctcgac ccgagcacgc cgtagaacgc gttcatgatg atcttcaatg
      421 cctgcgatag cgcgtggttg ccgtcgcgct tggcggtctc gcgctcctgc cacacctggg
      481 tgacgatggc cggcaggcag tgcttgtcgc gcgagaagcg cgcgccgcga aagccgggca
      541 cgaccgcatt ctcgtcggcg cttgcgccgc cttccaccag tccgaccgga tcgatcagga
      601 aggtgcggat gatggacggg tagaggctct tgtaatcgag caccagcacc gagtcgtaga
      661 gtccggagcg cgaatccatc acgaagccgc cggggctgtt ctcgcccgcg acgtcgccga
      721 ggttcggcgc gacatagccc tggcggtgca tcagcggcat gtacaggtgt tcgaaggccg
      781 ccaccgagcc gccgctgcgg tccgctgcca ggccggtcac gcttgcgcgt tcgagcagga
      841 agtccatcag cccggtgtgg tggaagatgc gcgtgacgag ttcgcagtcc ttcaggttgt
      901 aacgcgcgag cgccggcttg tcctcccgga agcgccgctc gatctcggcc atcctgtcat
      961 aaggattgtc gatcgccttg ccttcgccca gcagcgtctg cgccacatgt tcgagactga
     1021 acgaggcgaa gttccaggtc gcggacttca atgcttcgat cccgtcgatg accagccggc
     1081 ccgcgatgcc tgcgaagtaa tgctggcccc ggctgtgctc gcgccattcc atctcgctgc
     1141 cgtcgcgccc gaagcgcagc gcaatgccga gccgctgggc atgcttctgc agcaggcgca
     1201 ggtcgaactg cacgaggttc cagccgatga tcgcgtccgg gtcatgtcgc gccagccatg
     1261 cgttcaggcg ttcgagcagc tgcgcggggc tgtcgcaata cacgaggtcg aagtcgagcg
     1321 cgtcgccgcc gccgttgcgc gggcccagca tcagcacgtc gcgccgcccg cagccttcca
     1381 gcgcgatcga gtacagatcg ccgcgggcgc tggtttcgat gtcgagcgag accagcttca
     1441 ggcgcggacg gtatccgggg gcaggtttca tctgggcgtc gagcagcagg ccgctgccgt
     1501 cgcccgccgc gcgggcggaa aacgagaccg gcgcggtgat gaagcgctcc atcaggtagc
     1561 gttctggcgg gcgcacgtcg gcctcgaaga cgtcgacgcc gctttcgcgc aggcgctttt
     1621 ccagtttcag cagctggcgg tactgagcgc aatagagtcc gagcaccggg cggcgctgga
     1681 aatcgcgcag cgcaagctcg cgtagttcgc agccgcgttc cccgcgcagc gcggcctgcg
     1741 cctgctcgcg ctgcacggcc ggaatgaacg ccaccgaagg ctgggccgga acccgtacct
     1801 gtcgcgggcc ggcatcggtg gccagccaga actcgacttc cgtcccgccg ggcgtgtcac
     1861 gccagtgcct cgtcaacagg aacccttggt ctagctggga caatctgcag ccttttttgc
     1921 ccgcgcaccg ggccgatcgt tggagaagac gcgcctgcgc gactcgcgcg gcggcatgcg
     1981 tcgatcacag gattctatcc gggcgccgaa acaagcgatg tgccgcggtg actggatgtt
     2041 gaacagcgga ttgatgcagc acagccctgg tgatgttggt caattgtcag acaggaatgt
     2101 tgcctatagt tcctgaggca ttttttgatg tccgggggtt gccaatctca tataaacacg
     2161 ctgtttcaac accgatacag ggagagtcaa gatgcaattc gaagcatgcc ctgcatgcct
     2221 gggggagaac ataaaaacga ttcaccgcat cgccacgcgc gacctggaaa cgctgtatgg
     2281 gcgagaaggc ctggacgtgt cgcgctactt cggcgcgacc cgggagatca ggctgaacaa
     2341 gtgcctggcc tgcgatctcg gatttttctc gccgcattgc gccggcgacg atctgttcta
     2401 cgaacagctt cagaagttcg actggtacta ccaggatgac aagccggagt acgactatgc
     2461 gcgcaagctg gtcggcgatg cacagcgtgt gttggaggtc ggctgcggca agggagcgtt
     2521 cttctcctgg cttcccgaat cggtggaata cacgggactc gagttcaatg acgaggcgat
     2581 acgcaaggcg cgctcggccg gtctgaatgt gttcaagcag ccgatcgagg aacacgcgaa
     2641 gctggaagcc ggaaagtacg atgtcgtctg ttccttccag gtgctcgagc acattccggc
     2701 gccgcgcgga ttcatccgcg cctgcgtgca ggcgctcaag ccgggaggca agctcatctt
     2761 cgcggttccg gcggaggacg gtttcctcgg ggttgcggcg aacgcctacc tgaacatgcc
     2821 gccgcatcac gtgctgcgct ggagcgatcg ggcgctgcgg cacctggctt tccgtgaagg
     2881 gttgtcggtc atcgagctgt ggcatgagcc ggtggccgag tttcacaggg aatggcacca
     2941 ggagacgctg gcccggcact atcttgtcca gctcggcctc ggccagcagc gtctcgtgga
     3001 ccgcacgctc accggcaggg tgttctcgcg cctgcttcgc tcagggggca tcagagacta
     3061 cttcgccgcg aaggcggcaa aacggtttgc gccagcaaca catggccaca ccgtcgtact
     3121 cgtcgcgagc aagccggtct ggagcgaggt ccatgctgtg cagagaaagg cgagtccgga
     3181 tacggtggtt gcgcaatagc ctgcccgcgc aattcagctg tagtgccgcc cgcccactcc
     3241 gcggcgattc cggctcccac ctcgccccgg ctgttgctcc ggataccgcc gccattcgcg
     3301 ccgggctgtc cggttgatgc agcgcaaacg gcttgcagcc ccgggcggcc gccggccggt
     3361 cctgcgctga acccgtcctg gtgcggcttg tcttatcggc agtcgtcgca cttgatctca
     3421 aggaagcagg accatgaaca gagcaggagt agcccagggg aacggacgcg cgcagcaaca
     3481 ggcggttctg gccacgccga ccgatctgca ggacgcacag acccgggaca ttgccggcgc
     3541 gatgaatcgc gtgcttgccg atgtcttcgc gttgtacctg aagacgaaga atttccactg
     3601 gcacatgagc ggcccgcatt tccgcgatta tcacgaaatg ctggacgaac agagcgacca
     3661 gctgttcgcg atgaccgatc cgatcgccga gcgcgtccgg aaaatcggca agaccacctt
     3721 gcgctcgatc ggcgagatct cgcgcctgca gagcattcgc gacaatgacg ccgatttcgt
     3781 ctcgccgcag cagatgctgg ccgagctgtg cggagacaac aagacgctgg ccacacggct
     3841 gcgcgagacg cacgaggtct gcgacggcgg cagggacatc gccagcgcga gcctgatcga
     3901 aacctggatc gaggaaacgg agcggcgcac ctggtatctg ttcgaggcca gccgggcgcc
     3961 ggacaagacc gggcattgag agtcccggca agccacttgc cgcgttgggc cgaccttcgg
     4021 tcggctgttt gccgctgttg ctaacgcgcg cccggattct gctcgctgaa gtgcaggaaa
     4081 gccatccgat acagctcggc gccggaggcg atgccgagct tgcgcttgat ctgcgtgcgg
     4141 taggtttcga ccgtatgcgg cgacagcgaa aggcgctcgg cgatctccgt cttcttctgg
     4201 cccatgccga tcagctggaa cacttcgatc tcgcggtcgg agagcgtcgc gagcgcgttg
     4261 ctcgtcgtct cgccggtgcc cggcgacaag cggccgagca cgatcgtctg catcgcatca
     4321 gaaagccaga tgcgtcccgc gcataccgtc tccaccgcct tgacgaccga ggacgaggcg
     4381 ttgtgcttca tcatgtagcc ccgggcgccg gcacgcagcg cgcgttcggc atacagccgt
     4441 tcatcgtgca tcgaggtgac caggatcgcc atgccgggga cggcctcatg cagatccttg
     4501 accaagccca gcgaagtgac gccggccagc gacaggtcga ccagcgcgat gcgcggcctg
     4561 cgcgcggtgg ccagcgccag cgccgagacc gcatcctcgg cttcatagaa gcacatcgtc
     4621 ttgtgaagct ggccgagcag cgtggcaaac ccgacgcgca cgacagggtg atcgtcgacc
     4681 agcagcacgt ccagcacctc gctgtcggcc aggcctagca ttgcgtcacc ctgatgcatg
     4741 tgcccgtgcc cggcttcgat tcgacggcga gccgatatcc gatcaccgaa gcgcggtacc
     4801 gcatcacccg catccccgct ccctcgagcg cccacaagcc ggtgttcgtc ttgtccgcgt
     4861 cgcagtcgaa gccgatgccg tcgtcctgga tgctcagcat taccttgccc tccatgtaat
     4921 ccaggcgcag gtcgatgcgc gctgcattgc catgccggat cgcgttgctc accgcctcct
     4981 gcgcaatccg gtacaggtgc gcggcatcgg cactgtcgtc gatgtcgacc ggatcgtcga
     5041 aatcgaagct gcatgcgaca ccggtcatgg cctgcacgtc gaccgccagc tgcgcgagcg
     5101 cctcgcgcag gcccgacggc ccttccgcca ccggaagcag gccgtgcgcc agcttgcgga
     5161 cctcggaaat gcattcgctg cagcttcgct cgagcgcctt cgcccgtgga tgcagatgcg
     5221 gtgcggccgc ctgcatgtcc gcagcaagcg ccgccgctgc caggctcatg ccggtcagcc
     5281 gctgccccag gtcgtcgtgc agctcgttgc ctatgcgtct ttgctcgtcg tccgcgatcc
     5341 gcgcgacttc gcgctcgagc tcgcgcacgc gctcaagctc ggcgcgcagt tcggaggtcc
     5401 tggccgcgac cgcagtctcg agccttccgt tcgcatgcag ggtgcggcgt gaaagcacgc
     5461 tcagcagaac ggtgagcagg gcgccgaaca gaagcaccgc gggcggaagc gtttcgtacc
     5521 acgggttgac caggctgctg cgctccgcct gcacggtgcc ttgcaggtcg gcgccgccga
     5581 aatcgaacgc gaacgaagcg ctgatcatgc cgcgcggcgg atcgtcgacg ggcgccggcg
     5641 agctgcgatg cacaagcacg ggctgctcgt gcctggcgcc tgaagtgcgt ccaaggttgt
     5701 cgaccctgta tcgcagctgc ccggcccatt ggggcggcaa ggcttcggcg aacagcgcgg
     5761 gaacggtgaa cccgatgccg gcgctgccga tcagcacttc attgccggac gggtctggcc
     5821 ggtacacccc gagccgggca gcaatgcccg gcggggcgtt gcccggcggt gtgaagggga
     5881 ttgcagagct gtagaccgcg ttgacaggga acatcgcggg agtcacgagg atgggagggg
     5941 ccgacgggtc acgcgcgcgt tgtcgcgcat tctggacaat atccaatccc agtccgggcg
     6001 acttgctcgg ataaagatgc gtgatcacca tgaattccgg ctgtgactcg ggaatggcga
     6061 ggggcaggcg cttcctgccc tcggtatgcc gttcgaaatc ccggacgaat gcgtcgcgct
     6121 gcgcccgcgt gccgtatcgc gcgaagttga tgacggccag ggccgggtag cgttgcgcgg
     6181 cattcaggct cgtcgcatac gcctcgaact cctccatcgt gaccccgtcc cggctgcgaa
     6241 acaacccggc gacgccgaac aggatgtcct cgtagctgcg caggcggcgg atcagcgtgg
     6301 tctcgaagct ctttacctgg gacgcaaacg cgctttccgc ctcgtgatcg atctggcgct
     6361 gcacgctcca tgctccccag agcgtcacga tggtgccgac cacgagaatg atccggggga
     6421 gttgggcaga aatgcgcatg ttcgtgttgc agggtatgga ggcgctttgg cgtggccgtg
     6481 tgcgggagca ggttcaagac aggcatcgct ttttgagtga tgccgggctc cctattaaaa
     6541 gcgatgccag acctgactgc aagaaattat gcttcgaaaa gctgacaact gatgaccgaa
     6601 cagcatcccg tcagcatagg aatcaccgcg ccaggacacc tgatgacaac gactttcatc
     6661 gagccgattg acgccacatt ggtctgcgcc catcccccct ccgcccccgc gcttgcatcg
     6721 aagcggcttt atcgcctgaa ggccggcgag cgcatcggtc cgtactcgtt ccgcgaaatt
     6781 gcgcaataca tcggcgccac ccgcttcgct ccggatgaga tgatctggca cgagcg
//
LOCUS       DATLGF010000198         4585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_40131,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000198 DATLGF010000000
VERSION     DATLGF010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40131"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VM406_05980"
     CDS             <1..862
                     /locus_tag="VM406_05980"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HVL75546.1"
                     /translation="PRADVRRALTSFSAYAYLSKIASTLHQHADKVIVGTVGGPVALT
                     FYTVPVTLAGRILGLTYRLSSVIYPRASALAGAGRITELRPVYLGVMRYVTYINLAAL
                     GMIVLAGDEFLKRWVGAEFVQLGYPVLILFTLALLVDSLTNIPSMVNDALGHPRVTGK
                     FALANGVVGVALVYVGTVNWGIVGAAAGHLVSSAVMGTAFLLFVHGRTVPVAFIETLR
                     QGFGRSLAVGIAVIGVLMPLKWMLPQGVLTTLGVVALAGLTLALAGMLFIVGSDDRNA
                     MLAYARRLRS"
     gene            880..2103
                     /locus_tag="VM406_05985"
     CDS             880..2103
                     /locus_tag="VM406_05985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVL75547.1"
                     /translation="MRILLVSEDIPYPNMGGLAKHVLNLARALVRAGHEVDLLGGDQH
                     PIDVCGEEGRFGGRFFGELNGHHAGWKENSLGMFVPPKRSAIARRFARIILRRAAGYD
                     VIHYHGHVPNVGRYIPDHVNFVQTRHDQGSDCFRHTRFNSGRICASTDARDCAGCITN
                     QPNRLQRAVSAAAVVRFRREVADSFRRHKTVFVSDMLLKNFGRTMGPGPWGTTVHNFT
                     DRTELEKIRAAAAQLPPAPLFQVFIAAKLYPAKGIEPFLRAAVPRLRADMAITIAGDG
                     PDEARLRTEFESSRVQFLGWCAPDKTLKLAAASDAVVVPSVWEEPCATTVLEGLFLGK
                     PTFALARGGTPELAAYAAEPGQLRLHADMDSLVGDLMSFSRRAYGFAAPGLGSADGAA
                     RHLLDIYRQPPPRTT"
     gene            2117..2878
                     /locus_tag="VM406_05990"
     CDS             2117..2878
                     /locus_tag="VM406_05990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVL75548.1"
                     /translation="MPLTFSIITCTWNSARYLDESIASVLAQDYPDFEYIFVDGGSTD
                     GTLEKIRALDRPYQLLENVTGGVSRAMNQGLRAARGDVIAYLHSDDYYLRPDVLSLVA
                     RQLRLSGRGWLFGRTMSRVDAELRPDTYTAPRFSYSTLLRGNYIPHPATFVKRELMLA
                     AGGFNTSLKYAMDYDLWLRLARLDEPVQLDDALTAFREHEGSLSSSSRTRLAAMAEDF
                     RVRLSYCGRNPVRRLAHTARYLVRRQRIIQSMGNA"
     gene            2875..3732
                     /locus_tag="VM406_05995"
     CDS             2875..3732
                     /locus_tag="VM406_05995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HVL75549.1"
                     /translation="MRNPDISLILATVGRTDELDRLFESLAAQTYPNFEVIAVDQNED
                     DRLLPHLERARYLGIAVRHVRHSPPNLAAARNAGIEAARGRWIGFPDDDCWYDPRLLE
                     RLAARFSQEDHPHGIIVRWVEQDEQPLSAAALSWERSRAFRDIPVSSITLFCTRALCT
                     QIGGFDSRLGVGQWFGAGEETDFVLRALRAGAYLTYEPLGKVHHAVNPAKPIATPQTR
                     LAVRHRARGTGALYAKHRLPLWVIARGLLAPLLRPMLKGAFGSELALGYATMLGRLDG
                     LLGWHRRQT"
     gene            3933..>4585
                     /locus_tag="VM406_06000"
     CDS             3933..>4585
                     /locus_tag="VM406_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75550.1"
                     /translation="MEQHTEQHRPTHRSVAGPGGIQHRRAPVLRIALVTNHPPPFRIP
                     VYERIAALPDVEMYGIFCSRREPNREWEVPPLAFNHLFLKERFVARGSNFIHNNPDVF
                     ATLRRIAPDVIVTTGFNPTHLYAFGYALSHRIPHVPMTDGTDISEQALSRVHTLIRRF
                     VYSRSSAYIAASRGGLRLYRSYDIAPEHCFQSCLCIDNAAYLENDVAPPRFDLMFCGR
                     "
BASE COUNT          740 a         1611 c         1410 g          824 t
ORIGIN      
        1 gccgcgcgcg gacgtacggc gcgcgctcac gtcgttctcc gcatatgcct acctgagcaa
       61 gatcgcgtcg acgctgcacc agcatgccga caaggtcatc gtcgggaccg taggcggtcc
      121 tgtcgcgctg actttttaca cggtgccggt aacgcttgcc ggccgtatcc ttggactgac
      181 ctaccgtctg tcgtccgtga tctatccgcg cgcctcggcg ctggccggag ccggccgcat
      241 caccgagctg cgtccggtct acctgggcgt gatgcggtac gtgacctata tcaatctggc
      301 tgcgctgggc atgatcgttc tggcgggcga cgaattcctc aagcgctggg tcggggccga
      361 attcgtccag ctcggctacc cggtgctgat cctgtttacg ctggcgctgc tggtcgattc
      421 gctgaccaac atcccctcca tggtcaacga cgcgctcggc cacccgcgcg tgacgggcaa
      481 gttcgcgctg gccaacggcg tggtcggtgt ggcgctggtt tatgtcggca ccgtcaactg
      541 gggcattgtc ggtgccgcgg ccggccatct tgtttcctcc gctgtgatgg gcacggcatt
      601 tttgctgttc gtgcatggcc gtaccgtgcc ggtcgcgttc atcgaaacac tgcgtcaggg
      661 gttcggacgc agcctggcgg taggcatcgc tgtcatcggc gtgctgatgc cgctcaagtg
      721 gatgctgccg caaggcgtgc tcaccacact tggcgtggtg gcgctcgccg gcctgacgct
      781 cgcgctcgcc ggcatgctgt tcatcgtcgg cagcgacgac cgcaatgcca tgctcgccta
      841 cgcgcggcgg ctgcgttcct gaaagaccgg gttttcatca tgcgcatcct gctcgtttcc
      901 gaagacattc cctacccgaa catgggcggg ctcgccaagc acgtgctcaa cctcgcgcgc
      961 gcgctcgtgc gggccggaca cgaggtcgac ctcctcggcg gcgaccagca tccgatcgac
     1021 gtctgcggcg aggaaggccg gttcggcggg cgcttcttcg gggaactgaa tggccatcat
     1081 gccggctgga aggaaaacag cctgggcatg ttcgttccac ccaagcgcag cgcgatcgcg
     1141 cgccgcttcg cccgcatcat cctgcgccgg gccgccggct acgacgtcat tcattaccac
     1201 gggcacgtgc ccaacgtcgg gcgctacatt ccggaccacg tgaacttcgt gcagacccga
     1261 cacgaccagg gcagcgactg cttccgccat acccgcttca acagcgggcg gatctgcgcc
     1321 tccaccgatg cgcgcgactg cgccggctgc atcacgaacc agccgaaccg gctgcagcgc
     1381 gccgtctccg cggcggcagt cgtgcgcttc aggcgggaag tggccgacag tttcaggcgg
     1441 cacaagacgg tcttcgtctc cgacatgctg ctgaagaact tcggccgcac gatgggtccc
     1501 ggaccatggg gcacgaccgt tcacaacttc accgaccgca ccgagctgga aaagattcgc
     1561 gccgccgccg cgcaactgcc gccggccccg ttgttccagg tcttcatcgc cgccaagctg
     1621 tatccggcca agggaatcga accgttcctg cgcgccgcgg tgccgcggct gcgcgccgac
     1681 atggcgatca ccatcgccgg cgacggcccg gacgaagcgc gcctgcgcac cgagttcgaa
     1741 agcagccgcg tgcagttcct cgggtggtgc gcgccggaca agaccttgaa gctggcagcc
     1801 gcatccgacg ccgtggtcgt tccgtccgta tgggaagagc cctgcgcgac caccgtgctc
     1861 gaaggcctgt ttctcggcaa gccgacgttc gcgctcgcgc gcggcggcac gccggaactg
     1921 gcggcctacg ccgccgagcc cggacagctg cggctgcatg cggacatgga ttctctggtc
     1981 ggagacctga tgagcttttc acgccgcgcg tacggcttcg ccgcgcccgg cctgggcagc
     2041 gccgacggcg cggcacgcca cttgctggac atctaccgtc agccgccgcc ccgcactacc
     2101 tgacaggaga cgcaacatgc cactgacctt ttcgatcatc acctgcacct ggaacagcgc
     2161 acgctatctc gacgagtcga tcgcttccgt gctcgcgcag gattatccag attttgagta
     2221 catcttcgtc gacggcggct ccaccgacgg cacgctggag aaaatccggg cgctggaccg
     2281 tccgtaccag ctgctggaga acgtgaccgg cggcgtgagc cgggcgatga atcagggctt
     2341 gcgtgcggcg cgcggcgacg tgatcgccta cctgcattcg gacgactact acctgcgccc
     2401 ggacgtgctg agcctggttg cccgccaact gcgcctgtcc ggccgcggct ggctgttcgg
     2461 gcgcacgatg tcgcgggtgg acgcagagct gcgaccggac acctacaccg cgccgcgctt
     2521 ctcctacagc actctgctgc gcggtaacta catccctcac ccggccacct tcgtcaagcg
     2581 cgagctgatg ctcgccgccg gcggcttcaa tacctcgctg aagtatgcga tggactacga
     2641 cctctggctg cgtctggcac ggctggacga gccggtccag ctagatgacg cgctcaccgc
     2701 cttccgcgag cacgagggca gcctgtcgag ctcctcgcgc acccggctgg cggccatggc
     2761 cgaggacttc cgggtacgcc tgtcctattg cggccgcaat ccggtacgcc gcctcgcaca
     2821 taccgcccgc tacctggtgc ggcgccaacg catcatccag tcgatgggga acgcatgaga
     2881 aatccggaca tctcgctgat cctggccacc gttggccgca ccgatgagct cgaccgcctg
     2941 ttcgaatcgc tggcggcaca gacctacccg aacttcgaag tcatcgcggt agaccagaac
     3001 gaagatgacc ggctgctgcc gcacctggaa cgcgcgcgct acctcggcat cgccgtacgg
     3061 catgtgcgcc actcgccgcc gaatctcgcc gcagcgcgca atgccggcat cgaggcagcg
     3121 cgcgggcgct ggatcggctt tcctgacgac gactgctggt acgacccgcg cctgctcgag
     3181 cgtctcgccg cgcgcttctc gcaggaagat catccgcacg gcatcattgt gcgctgggtg
     3241 gagcaggacg agcagccgtt gtcggccgcc gccctttcct gggagcgatc gcgggcgttt
     3301 cgcgacatcc cggtcagttc catcacgctg ttctgcacgc gcgcgctgtg cacgcagatc
     3361 ggcggcttcg acagccgcct cggtgtcggc caatggttcg gcgccggtga ggaaaccgac
     3421 ttcgtgctgc gcgcattgcg ggccggcgcc tacctgactt atgaaccgct cggcaaggtt
     3481 catcacgctg taaatcctgc aaagccgatc gccactccgc agacgcgcct ggccgtgcgt
     3541 catcgcgcgc gcggcaccgg cgccctctat gccaagcacc gcctgccgct ctgggtcatc
     3601 gcgcgcggac tgctggcacc actcttgcgt cccatgctga agggcgcatt cggttccgaa
     3661 cttgcgctcg gctatgccac catgctcggg cgcctcgatg gcctgctcgg ctggcatcgc
     3721 cggcagacct gatttgattc gtcccaggct gcgaaggatt tgttacaaag cgaaccggca
     3781 agaacgagcg tgaattgatt ttccgccgcg cgtccagaga ggcaagcggc acaatcgaat
     3841 ccatgcaagc acgcactcaa tcacgctcca cgccgatcct gcagtccacg acaggcttgg
     3901 ttaccggtcc tgtcgcgctt actgacgacg agatggaaca acacaccgag cagcacaggc
     3961 ccacgcacag aagcgttgcc ggtccggggg gcatccagca ccgccgcgcc ccggtactgc
     4021 gcattgcgct ggtcacgaat catccgccgc cctttcgtat cccggtctat gagcgcatcg
     4081 ccgcgctgcc cgacgtggag atgtacggca tcttctgcag ccggcgcgaa cccaaccgcg
     4141 agtgggaggt tccaccgctg gccttcaacc atctgttcct gaaggagcgc ttcgttgcgc
     4201 gcgggtcgaa cttcattcac aacaatccgg atgtgttcgc gacactgcgc cgcatcgcgc
     4261 cggacgtcat tgtcaccacg ggtttcaatc cgacccacct gtacgcgttc ggctacgcac
     4321 tgtcgcaccg catccctcac gtgccgatga cagacggcac cgacatttcc gaacaggcgc
     4381 tgtcccgcgt gcacacgctg atccggcgct tcgtgtactc ccgctcgtca gcctatatcg
     4441 cagccagccg cggcggcctg cggctgtacc gcagctacga tattgccccc gaacactgct
     4501 ttcagtcctg cctgtgcatc gacaacgcgg cgtatctgga gaacgacgtc gcccccccgc
     4561 gcttcgacct gatgttctgc ggacg
//
LOCUS       DATLGF010000199         3458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_55156,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000199 DATLGF010000000
VERSION     DATLGF010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55156"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="VM406_06005"
     CDS             <1..1143
                     /locus_tag="VM406_06005"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HVL75551.1"
                     /translation="VVAFAMPPAVVAQAEQLAPFTGVPAKEGLELEEQRIWDESVGFD
                     RTLRRRGSLYGVAELDAYLQEVLDALYPEFKGAIRVRTVNNPDLNAFALPNGSIYVHL
                     GMLARLENEAQLAAVLAHEGAHFAYRHGLQRIQSVKGGAAFALVVGMVGGVAGAPDVG
                     SLLGQVALYSSTYGFSREQEREADRVGFERMVAVGYEPIQGARVFELLRDEAALLEEM
                     RPVMFSSHPKLEERIASFRELSSNHPEAANLTRTDVERFLSATALARADWLKQAFAFS
                     RHKSIIHVLEQAGAAQRFPAHYPFYLAEAYRLRNGDGDAEKALLAYRLALERAPEFAP
                     AYKSLGLVHMAAERKDEARAMFVQYLTLVPDADDAAFVTTYLQQLQ"
     gene            1154..1732
                     /locus_tag="VM406_06010"
     CDS             1154..1732
                     /locus_tag="VM406_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75552.1"
                     /translation="MSRRTSPARSAARAVSIALIVAALSACAVWTRIDTATQEGPGNR
                     YSVQAPIGWVRFSAAQDGIVITRDGMQIQHIVVMQKKDEEFFKKTKAKLPKAVMPSDL
                     AQLVLAELRSDPELSDLEVKEIAPYSIGAQAGFRVHLQFRNGRGALFDRVLIGAAREQ
                     TLTLMSYHALNTHYFARDVETFYKVASSFKAT"
     gene            complement(1830..>3458)
                     /gene="groL"
                     /locus_tag="VM406_06015"
     CDS             complement(1830..>3458)
                     /gene="groL"
                     /locus_tag="VM406_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238928.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HVL75553.1"
                     /translation="VFGDSARHKMVEGVNILANAVKVTLGPKGRNVVLERSFGAPTVT
                     KDGVSVAKEIELKDKLMNMGAQMVKEVASKTSDNAGDGTTTATVLAQAIVREGMKYVA
                     AGMNPMDLKRGIDKAVTATVEELRKIAKPCTTSKEIAQVGSISANSDSSIGDRIAEAM
                     EKVGKEGVITVEDGKSLQDELEIVEGMQFDRGYLSPYFINNPEKQVAVLENPFVLLYD
                     KKISNIRDLLPVLEQVAKASRPLLIIAEDIEGEALATLVVNNIRGILKTCAVKAPGFG
                     DRRKAMLEDIAILTGGQVIAEEVGLTLEKATLNDLGQAKRVEIGKENTTIIDGAGQGS
                     AIEARVKQIRVQIEESTSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVED
                     ALHATRAAVEEGIVPGGGVALLRARANVQSLKGDNADQDAGIKIVLRAIEEPLRMIVT
                     NAGEEASVVVNKVIEGKGNYGYNAATGEYGDMVEMGVLDPAKVTRSALQNAASIAGLM
                     LTTDCTVAELVEDKPAAAPAGMGGMGGMGGMDMM"
BASE COUNT          595 a         1069 c         1091 g          703 t
ORIGIN      
        1 gtcgtcgcct tcgccatgcc gccagcggtc gtcgctcagg cggaacagct cgcgccgttc
       61 accggtgttc ccgccaaaga gggcctggag ctcgaagagc agcgcatctg ggatgaatcc
      121 gtcggcttcg accggacact gcgccggcgt ggcagtctgt acggtgtcgc agagctggac
      181 gcctacctgc aagaggtttt ggatgcgcta taccccgagt tcaagggggc gatccgcgta
      241 cgaaccgtga acaatcccga cctcaatgcg tttgcacttc cgaacggcag catctacgtc
      301 cacctcggca tgctcgcgcg actcgaaaat gaagcgcagc ttgcggccgt actggcgcac
      361 gaaggcgcgc atttcgcata ccggcacggc ctgcagcgca tccaaagcgt caagggaggc
      421 gccgccttcg cgcttgtcgt cggcatggtc ggaggtgttg cgggagcacc tgacgtggga
      481 agtctgctgg ggcaggtcgc gctgtattcg tccacgtacg gcttttctcg ggagcaggag
      541 cgcgaggccg accgcgtggg attcgagcgc atggtggcgg tcggctatga gccaatccag
      601 ggagcccgtg tgttcgaact gctgcgcgac gaggccgctc ttctcgagga aatgcggccg
      661 gtcatgtttt cttcccatcc gaagctggaa gagcggatag ccagtttcag ggagctgagc
      721 agcaaccacc cggaagccgc aaacctgaca cgtaccgacg tcgagcgatt tctgtcggcg
      781 acagcgctgg cgcgtgcgga ctggctgaag caggcgttcg ctttctcgcg ccacaagagc
      841 atcattcacg tgctcgagca ggccggtgcg gcgcagcgct ttcctgccca ctatccgttt
      901 taccttgccg aggcctatcg cctgcgcaac ggggacggcg atgcggaaaa ggcactgctg
      961 gcctatcggc tggccctgga gcgcgctccc gaattcgcac ccgcctacaa gtcgctcggc
     1021 cttgtccaca tggctgcgga gcgcaaggac gaggcccgcg cgatgttcgt gcagtatctg
     1081 acgctggtac cggatgcgga tgacgcggcc ttcgttacta cctacttgca gcaacttcaa
     1141 taggggagaa tccatgagca gaagaacgtc gccggcccgt agcgcagcaa gggccgtctc
     1201 gattgccttg attgtggccg cattgagcgc atgtgccgtg tggacccgga tcgacactgc
     1261 gacccaggaa gggccgggta accggtacag cgtccaggca ccaatcggct gggttcgctt
     1321 cagtgccgcg caagacggaa tcgtgataac ccgtgacgga atgcagatac agcacatcgt
     1381 cgtcatgcag aagaaggacg aggagttctt caagaagacg aaggcgaaat tgccgaaggc
     1441 cgtgatgccc tccgatcttg cgcagttggt gctggcagaa ctccgctcgg atccggaact
     1501 ctccgatctt gaagtcaagg aaatcgcgcc gtactcaatc ggagctcagg caggcttccg
     1561 cgtacacctc cagttccgta acggccgcgg cgccctgttc gatcgcgtcc tgatcggtgc
     1621 agccagggaa cagacgctta cgctgatgag ttatcacgcg ctgaacaccc attacttcgc
     1681 gcgtgatgtg gaaacgttct acaaggtggc aagctcgttc aaggcaacct gaagccgccg
     1741 ctgccctttc aaaaaaaaac ccgccgaagc gggttttttt gtttggcgcg aatcccggca
     1801 cagggccgga aacccgccgg gccgaacaat tacatcatgt ccatgccgcc catgccgccc
     1861 atgccgccca tgcctgccgg agcagcggcc ggcttgtctt cgaccagctc ggccaccgtg
     1921 cagtcggtgg tcagcatcag gccggcgatc gaagcggcgt tctgcagcgc ggagcgggtg
     1981 accttggccg gatccagcac gcccatctcg accatgtcgc cgtactcgcc ggtcgcggcg
     2041 ttgtagccgt agttgccctt gccttcgatg accttgttca cgacgaccga agcttcctcg
     2101 ccggcgttgg tgacgatcat gcgcagcggc tcttcgatcg cgcgcagcac gatcttgatg
     2161 ccggcgtcct gatcggcgtt gtcgcccttc aggctttgca cgttggcgcg ggcgcgcagc
     2221 agggccacgc cgccgccggg cacgatgcct tcttcaacgg cggcacgggt ggcgtgcagc
     2281 gcgtcttcga cacgggcctt cttttccttc atttcgactt cggtggctgc gccaaccttg
     2341 atcacggcaa cgccgccggc cagcttggcc acgcgctctt gcagcttctc gcggtcgtag
     2401 tcggaggtgg actcttcgat ctgcacgcgg atctgcttga cgcgcgcttc aatcgcggaa
     2461 ccctggccgg cgccgtcgat gatcgtggtg ttctccttgc cgatctcgac gcgcttggcc
     2521 tggccgaggt cgttcagcgt ggccttctcc agcgtcaggc cgacttcctc ggcgatcacc
     2581 tggccgccgg tcaggatggc gatatcttcc agcatggcct tgcgacggtc gccgaagccc
     2641 ggagccttga cggcgcaagt cttcaggatg ccgcggatat tgttcacgac cagagtggcc
     2701 agcgcttcgc cttcgatgtc ttcggcaatg atcaagagcg gacgggaagc cttggcgact
     2761 tgttccagaa ccggcagcag gtcacggatg ttggagatct tcttgtcgta cagcaggacg
     2821 aacgggttct ccaggacggc gacttgcttt tccgggttgt tgatgaagta cggggacagg
     2881 tagccgcggt cgaactgcat gccttccacg atttccagct cgtcctgcag cgacttgccg
     2941 tcttcgacgg taatcacgcc ttccttgccg accttttcca tggcttcagc aatgcggtcg
     3001 ccgatcgagg agtcggagtt ggccgagatc gagccaacct gggcgatttc cttcgaggtg
     3061 gtgcagggct tggcgatctt gcgcagttct tcgacggtcg cggtgacagc cttgtcgatg
     3121 ccgcgcttca ggtccatcgg gttcatgccg gcggccacgt acttcatgcc ttcgcgcacg
     3181 atggcttgtg ccagaaccgt tgcggtggtg gtgccgtcac cggcgttgtc ggaggtcttg
     3241 gaagcgactt ccttgaccat ctgcgcgccc atgttcatga gcttgtcttt gagttcgatt
     3301 tccttggcga ccgagacacc gtccttggtc acggtcgggg cgccgaacga gcgctccagc
     3361 acgacgttgc ggcctttcgg gcccagggtg accttgactg cgttggcgag gatgttgacg
     3421 ccttcgacca tcttgtggcg ggcggaatcg ccgaatac
//
LOCUS       DATLGF010000200         6705 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_164689, whole genome shotgun sequence.
ACCESSION   DATLGF010000200 DATLGF010000000
VERSION     DATLGF010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6705
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164689"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VM406_06020"
     CDS             complement(<1..218)
                     /locus_tag="VM406_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75554.1"
                     /translation="MHEIRIESVGDLSREVRGLLSSDRKWLFRGQSDACWELLPSVLR
                     GYTAQQERYLTNEFRVRARSRYAPCPAN"
     gene            complement(259..1242)
                     /locus_tag="VM406_06025"
     CDS             complement(259..1242)
                     /locus_tag="VM406_06025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVL75555.1"
                     /translation="MTPLSVLDLAPIVEGGTAADSFRNSVELAQHCERLGYRRFWLAE
                     HHSMPGVASAATAVLIAHVAAHTGRIRVGAGGIMLPNHAPLVIAEQFGTLASLFPGRI
                     DLGLGRAPGSDQATARALRRNLDTDPDAFPQDVLELMHYFAPEQPGQRVRAVPGAGLD
                     VPIWILGSSLFGAQLAARLGLPFAFASHFAPAQMMDAIALYRSLFQPSDKLDKPYVML
                     GVSVFAADTDEEAHLLATSMQQAFVNLRSGRPTRLQPPVPGYADALNEHERAILAHTM
                     SCSIIGSAATVATGIKDFIARTGADELMIASQIYDHRARLHAYTLAAQAMN"
     gene            1438..4254
                     /locus_tag="VM406_06030"
     CDS             1438..4254
                     /locus_tag="VM406_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVL75556.1"
                     /translation="MPTDWSPPHPVPQAQYRQIIDSVRDFAIISMDPARRITTWSAGA
                     TAVLSWTEAEMLGRSADRIFTEEDCAAGVPAVEAQTALANGMASDERWHLRKDGERFW
                     AVGELTPLHDENGGHAGFVKILQDRTGQKHAQARLKALNQELEALVAERTRERDRIWR
                     NALDLMLVIDGEGVLRGVNPAWTRQLGYAAEDLVDQHFESFVHPADIGPTTEAIAHAL
                     NRPLVHFEVRIRSKAGEYHWFAWTAAPEEGLIYANGRDITMEKRQAEQLLMANEARLQ
                     LALEAGEMGAWEWDMQSDEILFLQGNTTLHGLPPTDEPVRRLEMQDYVGMVDPQDRPA
                     LAEILGRARAGERKLRTEYRIRRADGSLQWLEARGTVVADDDGRPLRMTGVSVNITGR
                     KRTEQDLQFLARASAELSGLVDPQSSLDTLAFLSVPAFADWCAIDLLNEAGELERVSV
                     AHVDPAKVKLARELHERTPPDREQRHGVWNVMKSGQPQLVRMVTDEVLDQTVRDERYR
                     AALKELGLRSYIGVPLLAHGRTMGVLTFISAESGRIYDGHDLEIAADLARRAAVAVEN
                     ALLYRALQRSDQGKDVFLATLAHELRNPLAAIANGLGILRLAPGDIRRVEHAGVIMER
                     QVGQLRRLVDDLMDVSRIATGKIELRKEETDLVAVLANAIETTRPAIESAHHKLSIAL
                     PGGSTRLYADPVRLSQVFGNLLNNAAKYTDDNGEIEIGMDVTPEEFIVRVRDNGIGIE
                     PDMQKSIFTIFRQGAHPVERTHGGLGIGLSLVDGLVRLHGGRVEVFSEGRGKGSEFSV
                     HLPRVEAPAQAAPLEAARERANDVVRRVLVVDDNVDAANTITELLRLLGHEVDIEHDG
                     LSAVDAALRGRPDVVLLDIGLPGIDGYEAARRIREAEGVHRIKLVALTGWGQEQDRDR
                     AFRAGFDEHCVKPVGLDQLRIVLG"
     gene            4266..4481
                     /locus_tag="VM406_06035"
     CDS             4266..4481
                     /locus_tag="VM406_06035"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HVL75557.1"
                     /translation="MTIEQFIDVGLAEIARMMLEAEGIPVLLHSAAHASLIGGLAVGG
                     IRLQVPREYAPKAREILRQLNDNGAEG"
     gene            complement(4518..6698)
                     /locus_tag="VM406_06040"
     CDS             complement(4518..6698)
                     /locus_tag="VM406_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HVL75558.1"
                     /translation="MDADDTGFNDSGNPDFNSVLNARMSRRDMMRVSAVSAGTALLGS
                     LGLSACGGSDAVTETEVGGSEETLLGFNAVAKSLADTLVVPAGYTATVLYALGDPLNA
                     STPAFKNDGTDTSFDTRAGDHHDGIEYFPLSGAGTPSKTSSDRALLAMNHEATTDETR
                     SSFFLHADGGTTTLPRPASEIDKEVDIHGVSVVEIRKTNNTWATVQNSAFNRRFTPLT
                     EMDLSGPVRGNALVQTVFSTNGTLCRGTLNNCGTGKTPWGTLITGEENWGGYFTRGSG
                     DNAARGNDKSVTALNRYGRAQGAASRHGWESGGEARHARWDISASGASAIADYRNELN
                     TFGYIVEIDPYNATSRAKKRTALGRFAHENGAFGKLVAGQPLAVYMGDDSRGEYMYKF
                     VSQVAWNPVDADPANRMATGDKYLDSGRLYVARFNADGSGEWIELNISNAQISGYANY
                     TFTDQADVLVNARLAADAVGATRMDRPEWCNVHPTTGEIYYTLTNNSQRTTAAGNNQV
                     NAANPRAYTDIKGTSQTQNGNVNGHIIRIREGAAGNAATAFTWDVYLFGAEAGADPTR
                     INLSGLTSDQDFSSPDGLVFSESTGICWIQTDDGAYTDVTNCMMLAALPGQVGDGAPV
                     TINYGGGVTVTTPVGAKPTQRTLKRFLVGPKGSEITGICESPDGRAIFVNIQHPGENT
                     LQADIGDPGKYQSQWPANAGYGAGNRPRSATVVITRNDGGRVGT"
BASE COUNT         1081 a         2119 c         2382 g         1123 t
ORIGIN      
        1 ttgttggcgg gacagggcgc atagcggctg cgcgcgcgca cccggaactc gttggtcagg
       61 tagcgttcct gctgcgcggt gtagccgcgc agcacgctgg gcagcaattc ccagcaggcg
      121 tcggactggc cgcggaacag ccatttcctg tcggaactga gcagaccccg gacttcgcgc
      181 gagaggtcgc ccacgctttc tattcggatt tcatgcatcg ggtccactcc tcgccggttc
      241 gacccgccgc gtgtcgcgct agttcattgc ctgcgcggca agggtgtacg cgtgcaggcg
      301 tgcgcggtgg tcataaatct gcgaggcgat catcagttcg tccgccccgg tgcgcgcgat
      361 gaagtccttg atccctgtgg cgaccgtcgc cgccgacccg atgatcgagc acgacatcgt
      421 gtgggccagg atcgcgcgtt cgtgttcgtt cagcgcatcg gcatagcccg gcaccggcgg
      481 ctgcagccgg gtcggacggc cgctgcgcag gttcacgaag gcctgctgca tcgaggtcgc
      541 cagcagatgc gcttcctcgt cggtgtcggc ggcgaacacg gacacgccca gcatcacgta
      601 gggcttgtcc agcttgtcgg aaggctggaa cagcgagcgg tacaacgcaa tcgcatccat
      661 catctgggcc ggcgcgaaat gcgaggcaaa cgcgaacggc aggcccagcc gcgcggcgag
      721 ctgcgcgccg aacaggctgg agccgaggat ccagatcggc acgtccaggc cggcgccggg
      781 cacggcgcgc accctttggc ccggctgctc cggcgcgaag tagtgcatca gctccagcac
      841 gtcctgcggg aaggcgtccg ggtcggtgtc gaggttgcga cgcagcgcgc gggcagtggc
      901 ctggtccgag ccgggcgcgc ggcccaggcc gaggtcgatg cgacccggaa acagcgatgc
      961 cagcgtgccg aactgttcgg cgatcaccag cggcgcgtgg ttgggcagca tgatgccgcc
     1021 ggcgccgacg cggatgcgcc cggtgtgcgc ggccacatgg gcgatcagca ccgcggtggc
     1081 ggcgctggcg acgccgggca tgctgtggtg ttcggccagc cagaaacggc ggtaaccgag
     1141 acgttcgcag tgctgcgcca gttcgacgct gttgcggaag gaatcggcgg cagtgccgcc
     1201 ttcgacgatg ggggcgaggt cgaggaccga gagtggtgtc atgacttgaa ggattgccgt
     1261 gtaaggacct gattctagag cctgtcgtga actgtttcag gaagcgcggc cttgtgcgca
     1321 tgctgtggcg cccgcccgag acaagccgca acaaaactgt gcgttcatga tcgaatgcaa
     1381 gagggcaagc cctttcctgc cgcacatttg cctcaaccac atccaggccg catccccatg
     1441 ccgacagact ggtctccacc gcatcccgtc ccgcaagcgc aataccgtca gatcatcgac
     1501 agcgtccgcg atttcgccat catcagcatg gacccggcac gtcggatcac gacctggagc
     1561 gcgggggcta ccgccgtcct tagctggacc gaggccgaga tgctcggccg cagcgcggac
     1621 cgcattttca cggaggaaga ttgcgcggcc ggcgtgccgg ccgtcgaggc gcagacggcg
     1681 ctggcgaacg gcatggcgtc ggacgaacgc tggcacctgc gcaaggacgg cgagcgtttc
     1741 tgggcggtag gcgaactcac tccgctgcat gacgaaaatg gcggccacgc cggcttcgtc
     1801 aagatcctgc aggaccgcac cggacagaag cacgcgcagg cccgattgaa ggcgctgaac
     1861 caggagctgg aggcactggt cgccgagcgc acgcgcgagc gcgaccgcat ctggcgcaat
     1921 gcactcgacc tgatgctggt gatcgacggc gagggcgtgc tgcgcggcgt caatcccgca
     1981 tggacgcggc aactcggcta tgcggcggaa gacctggtcg accagcactt cgaaagcttc
     2041 gtgcatccgg ccgacatcgg cccgaccacc gaagcgatag cgcacgcgct gaaccggccg
     2101 cttgtccact tcgaggtgcg catcaggagc aaggccgggg agtaccactg gttcgcgtgg
     2161 accgccgcgc ccgaagaagg actgatttac gcgaacggcc gcgacatcac gatggaaaag
     2221 cgccaggccg agcaattgct catggccaac gaagcccggc tgcaactggc gctcgaggcc
     2281 ggcgagatgg gcgcctggga atgggacatg cagagcgacg aaattctgtt cttgcaaggg
     2341 aacacgacgc tgcatggcct gcctcccacc gatgaaccgg tgcgtcggct ggagatgcag
     2401 gactacgttg gcatggtcga tccgcaggac cgaccggcgc tggccgagat cttgggccgc
     2461 gcccgcgccg gcgaacgcaa gctgcgcacc gagtaccgca tccgccgcgc ggacggctcg
     2521 ctgcagtggc tggaggcgcg cggcaccgtg gttgcagacg acgacggccg gccgctgcgc
     2581 atgacggggg tcagcgtcaa catcaccggg cgcaagcgga ccgagcagga cctgcagttt
     2641 ctcgcgcgcg ccagcgcgga gctgtccggc ctggtggacc cgcaaagttc gctcgacacg
     2701 ctcgcctttc tgtcggtgcc ggcgttcgca gactggtgcg cgatcgacct gctgaacgag
     2761 gcgggcgagc tcgaacgggt ctcggtggcc catgtcgatc ccgccaaggt gaagctcgcg
     2821 cgcgaactgc atgaacgcac cccgcccgac cgcgagcagc gacatggcgt gtggaacgtg
     2881 atgaagtccg gccagccgca gctggttcgc atggtcaccg acgaagtgct tgaccagacc
     2941 gtgcgcgacg agcgctaccg cgccgcgctg aaagaactcg gcctgcgctc atacatcggc
     3001 gtgccgcttc tcgcgcacgg ccgcacgatg ggggtgctca ccttcatcag cgccgaatcg
     3061 gggcgcatat acgacggcca cgatctcgag atcgcggccg atctcgcgcg gcgcgcggcc
     3121 gtcgccgtcg agaatgcgct cctgtaccgc gcgctgcagc gctcggatca gggcaaggat
     3181 gtgttccttg ccacgctcgc ccacgagctg cgcaacccgc ttgccgcgat cgcgaacggt
     3241 cttggcatcc tgcggctggc gccgggcgac atacggcgcg tcgagcatgc cggggtcatc
     3301 atggagcgcc aggtcggcca gttgcggcgc ctggtcgacg acctgatgga cgtttcgcgc
     3361 atcgcgaccg gcaagatcga gctgcgcaag gaagagaccg atctcgtggc cgtgctcgcg
     3421 aatgcgatcg aaaccacgcg tccggcgatc gagtccgcgc atcacaagct gtccattgca
     3481 ctgccgggcg ggtcgacgcg cctttacgcc gatccggtgc ggctgtcgca ggtattcggc
     3541 aatctgctca acaacgccgc caagtacacc gacgacaacg gagagatcga gatcggcatg
     3601 gatgtcactc cggaggaatt catcgtgcgc gtgcgggaca acggcatcgg tatcgagccg
     3661 gacatgcaga aaagcatctt cacgatcttt cggcagggcg cgcatccggt ggagcgtacg
     3721 cacggcgggc tgggcatcgg actgtcgctg gtggacgggc tggtacgcct gcacggcgga
     3781 cgggtcgagg tgttcagtga aggacgcgga aaaggcagcg agttctcggt gcatctgccg
     3841 cgcgtcgagg cgccggcaca ggcggcgccg ctcgaagcgg cgcgcgagcg tgcgaacgat
     3901 gtggtgcgcc gcgtgctggt cgtggatgac aacgtcgacg ccgccaatac gatcaccgaa
     3961 ctgctgcgcc tgctgggcca cgaggtggat atcgagcatg acggcctgtc ggcggtggac
     4021 gccgcgctgc gcgggcgccc ggatgtcgtg ctgctcgata tcggcctgcc cggcatcgac
     4081 ggctatgaag cggcgcgccg catccgcgaa gccgaaggcg tgcaccgcat caagctggtc
     4141 gcgctgaccg gctggggcca ggagcaggat cgcgaccggg cgttccgggc gggcttcgac
     4201 gagcattgcg tcaagccggt gggactcgat cagctgcgca tcgtgttggg gtagcaatgg
     4261 agctggtgac gatcgaacaa ttcatcgatg tcggcctggc ggagattgca cgcatgatgc
     4321 tcgaagccga aggcatcccg gtgctgctgc attcggcggc tcatgcgtcg ctgatcgggg
     4381 gacttgcggt gggcggcatt cggctgcagg tgccgcgcga gtatgcgccg aaggcgcggg
     4441 agatcctgcg ccagctcaat gacaacgggg cggaaggctg atgccgtccg ccccgtccat
     4501 tcacgtgtca ggctggatca ggtgcccacg cggccgccgt cgttcctggt gatcacgacg
     4561 gtcgccgaac gcggacggtt gccggcgccg tagccggcgt tggccggcca ctggctctgg
     4621 tacttgcccg ggtcgccgat gtccgcctgc agtgtgttct cgcccgggtg ctgaatgttg
     4681 acgaagatcg cgcgtccgtc cgggctttcg cagatgccgg tgatctcgct ccccttcgga
     4741 ccgaccagga aacgcttgag cgtgcgctgc gtcggcttgg cgccgaccgg cgtcgtgact
     4801 gtcacgccgc ctccgtagtt gatcgtcacg ggcgcgccat cgccgacctg gcccggcagc
     4861 gcggccagca tcatgcagtt ggtcacgtcg gtgtacgcac cgtcgtcggt ctggatccag
     4921 cagatgccgg tcgattcgga gaacaccagg ccgtccgggc tggagaaatc ctggtcggag
     4981 gtcaggccgg agaggttgat gcgggtcggg tccgcgccgg cttcggcgcc gaacaggtag
     5041 acatcccagg tgaatgcggt cgcggcattg ccggccgcgc cttcgcggat gcggatgatg
     5101 tggccgttga cgttgccgtt ctgggtctgc gaggtgccct tgatatcggt ataggcgcgc
     5161 gggttggcgg cgttgacctg gttgttgccg gcggcggtgg tgcgctgcga gttgttggtc
     5221 agcgtgtagt agatctcgcc ggtggtcggg tgcacgttgc accattccgg acggtccatg
     5281 cgggtcgcgc cgaccgcgtc cgccgccaga cgggcgttca ccagcacgtc ggcctggtcg
     5341 gtgaacgtgt agttggcata accggagatc tgcgcattgc tgatgttcag ctcgatccac
     5401 tcgccgctgc catcggcgtt gaagcgcgct acatacaggc ggccgctgtc gaggtacttg
     5461 tcgccggtgg ccatgcggtt ggccggatcg gcgtcgactg ggttccacgc cacctgcgag
     5521 acgaacttgt acatgtattc gccgcgcgag tcgtcgccca tgtagaccgc cagcggctgg
     5581 ccggcgacca gcttgccgaa cgcgccgttc tcgtgcgcga agcggcccag cgcagtgcgc
     5641 ttcttcgcgc gcgatgtcgc gttgtacgga tcgatctcga cgatgtagcc gaaggtgttc
     5701 agttcattgc ggtagtcagc gatcgccgac gcgcccgagg cgctgatgtc ccagcgcgca
     5761 tgacgcgctt cgccgccgct ttcccagcca tggcgggagg ctgcgccctg ggcgcggccg
     5821 tagcggttga gcgcggtcac gctcttgtcg ttgccgcgtg cggcgttgtc gccgctgccg
     5881 cgggtgaaat agccgcccca gttctcctcg ccggtgatca gcgtgcccca cggggtcttg
     5941 ccggtgccgc agttgttcag cgtgccgcgg cacagcgtgc cgttggtcga gaacacggtc
     6001 tgcaccagcg cgttgccgcg caccgggccg ctcaggtcca tctcggtgag cggcgtgaag
     6061 cggcggttga acgccgagtt ctgcaccgtc gcccaggtgt tgttggtctt gcggatctcg
     6121 acgacggaca cgccgtggat gtcgacctcc ttgtcgattt cggaagccgg gcgcggcagc
     6181 gtggtggtgc cgccgtcggc atgcaggaag aacgaggagc gggtctcgtc ggtggtcgcc
     6241 tcgtggttca tcgccagcag ggcacggtcc gaggatgttt tcgacggcgt gccggcgccg
     6301 gagagcggga agtactcgat gccgtcgtgg tggtcgccgg cgcgggtgtc aaagctggtg
     6361 tcggtgccat cgttcttgaa cgccggggtc gaggcattga gcgggtcgcc cagcgcatag
     6421 agcacggtag cggtatagcc ggcgggcacg acgagggtgt cggccaggct cttggctacc
     6481 gcgttgaagc ccagcagggt ttcctcgctg ccgccgactt ccgtttccgt caccgcgtcc
     6541 gagccgccgc aggcggacag gccgagcgag ccgagcagcg cggtgccggc cgaaacggcc
     6601 gagacgcgca tcatgtcgcg gcggctcatg cgcgcattca gcacggaatt gaagtcgggg
     6661 ttgcccgaat cgttgaagcc ggtgtcgtcg gcatcaatct tgacg
//
LOCUS       DATLGF010000201         7786 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_40219,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000201 DATLGF010000000
VERSION     DATLGF010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 7786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7786
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40219"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            500..1528
                     /locus_tag="VM406_06045"
     CDS             500..1528
                     /locus_tag="VM406_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HVL75559.1"
                     /translation="MEAANSAPIPATLIPGDGIGPEVTDATVQVLNALGSPFEWDTQM
                     AGMAAMDASGDPLPAAVLDSIRRTRLALKGPLTTPIGGGFRSVNVRLREEFKLYANLR
                     PVRTIIPGRYDNIDIVLVRENIEGLYVAHEYFVPVGDDPRAVAVSTGVNTRDGCRRIA
                     RYAFEYAVKNGRKKITIVHKANVLKALTGIFLEAAREVAQEYAGRVEVDDRIVDACAM
                     QLVLNPWQFDMLLCTNLFGDILSDQLAGLVGGLGLAPGANIGEQAAIFEAVHGSAPDI
                     AGKGLANPISLMLAAALMLDHVGKPELATRLRDAIQATLLEDNVRTRDLGGTASTAQF
                     TAALLKRL"
     gene            complement(1592..2476)
                     /locus_tag="VM406_06050"
     CDS             complement(1592..2476)
                     /locus_tag="VM406_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75560.1"
                     /translation="MRELVFIHGRAQHGKDANALKQEWIDAWGEGLAKSGLTMPISPS
                     QIRFPYYGDTLDQLVQGVSDGERAEVVVRGLDIGDDEKSFIQEYVGEVQEKCEAIGSP
                     LPAALPDVEYGERGVLNWPWVQAILRMLDGVSPASKASVALFANDVFRYLHANEIRHV
                     IDEGVRQAFARDTDTVVVGHSLGSVVAYNVLHRYGPQAGWRVPLFVTLGSPLAVSAIR
                     RRLVPAPRHPECVRDWFNALDPRDVVALWPLDGERFMVNPAIENKTDVDNFTENRHGI
                     RGYLSDADVAYRIYAHLT"
     gene            complement(2513..5461)
                     /locus_tag="VM406_06055"
     CDS             complement(2513..5461)
                     /locus_tag="VM406_06055"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HVL75561.1"
                     /translation="MNALIAGSQSEPLDISVMNGNLRFVRGALMVGHYRSTSLTGTER
                     ILNLMYGEALSNSLRIGGYPHRPGDRQLFRNPNLCREDAYLPRQPETIIVVGLGQEGK
                     LSVPEHVATVRQGVVAWVRETVALATTAPQSLTLTTTLLGSGGAGVSAGQAAQAIAQG
                     VRDANSRLCEVGYPCIKHLRIIEIYLDRATEAWRALEVLENGGAPPGQFKLQKTVEAG
                     VGAMQRPLDSSYRGARHDLIRALTRVEAPDRRFIEYSLDTRRARAELRAQHTQDRLLQ
                     ELIATASRKSVLDNRIGQALFKLVVPAEMEPFLGGTTEVQIELDDGTACIPWELLDTS
                     AGAQDQGDAEPWAIRVKLLRRLLTADFRSEVMDADSASDVLVIGEPKCDPLRYPRLPG
                     AREEAKAVAKRIKEKLGAERVMDLVSDGQGANEIDARRVITTLLSRDWRVIHIAGHGE
                     PADQDGDPRGVVLSSGIYLGPREIRNMRQVPQLVFINCCHSAAAHASDLLASPQPSTY
                     GRAGFAANIAKSLIKIGVRCVVAAGWIVEDRAAREFATTFYKALLDGKRFIDAVAEAR
                     KAAWDKFGMTNTTWAAYQCYGDPDWRLQNERDADERREPPHAQEFAGISTSSALVIAL
                     EAIVTNAKYEKRSPARQSAKIRYLAENYGKQWECIGSVAEAFGLAYAAVGEFNAARTY
                     LERAIASPDASASMRAGEQLAKVYGQQAWKVFQDAVAHIEPDPEGRDGPEDRSNSGAY
                     ERAHESASAQLNKARALLEKLCMFQATAKRHSLCAYICKRIAMVEAIAGQDWKEKDAT
                     ENMKEQCGKALELAAAIEAADQHYPALNLLAAEINLHGEAVSWETVAPLAQKAMSCGT
                     NPAGHTYLDFRSHLIKVELLIYESVVRQQLAGNLERIMQRLSELHEYDNALERWQSVR
                     DNGWFVLSKYGKRARPDEQRAAATLLLELDRRAGLPALHARVPAGEGARNGEGDTQEG
                     IDRALPQTVP"
     gene            complement(5949..6125)
                     /locus_tag="VM406_06060"
                     /pseudo
     CDS             complement(5949..6125)
                     /locus_tag="VM406_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
     gene            6130..6243
                     /locus_tag="VM406_06065"
                     /pseudo
     CDS             6130..6243
                     /locus_tag="VM406_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448234.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(6522..7421)
                     /locus_tag="VM406_06070"
     CDS             complement(6522..7421)
                     /locus_tag="VM406_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75562.1"
                     /translation="MLKMKNAALATAVLLALAACGGSSPDDADPGLDNGTPVIPGTGP
                     GTDPDPDPGTDPGTDPAPADDTFAGSIVMRSNQVLFNGGDYVNAQDYAVFSTTGVYAQ
                     ESRAAGASTPLSTFGMRVDQIGMNGQSGTIRVAVEFDNTASDELFQFLVEGATLTINE
                     TGRMSIASPDTARAYVVVNNGSGASANVTVAEVPADAVTVSTRPVHDHDPSSFALLVN
                     LDAIMQAAIAAATDADRAVLAGASNANASGRYTLRMGLTGITLENDSGNAITLESPIT
                     VGSRAPLGGAGVSGVLYHNVEYP"
BASE COUNT         1373 a         2485 c         2352 g         1576 t
ORIGIN      
        1 ctatgtaatc cagcgattcc gccgtcgtaa gcgaggcgtg tgtcgtgccg ttgtgatcgg
       61 taaatttttt taccggcaga cccgccacga catcggtgac aacatagttc aggcaagtca
      121 gcaagtacga tttcttcaag acctctctca tcgcgccgct cctgtcggtt ctgtgaaatc
      181 atttcagccg cgcggcacct cctcgtccgc ggggatgacg ttgatcctgc atttccccga
      241 attcaagctg ccggagcgcg caggtgcgga ggctcgacag ggttttcatt gtcggcgcag
      301 gtaaaccggc gaacaatggt tccgctctcc cgtcaagcgc gcgactgatc tgcgtcagtt
      361 tcatatttgc gcatcgattt gcctgttgac gcatttcgca gcggaacaag attgtcgtgg
      421 cgaagaaggc ctcctgacag gcaaggatgg tatgttcatc cgattcaacc tcaaaccttc
      481 aactgccctg gagaaagcca tggaagcagc gaacagcgca ccgatccccg ccaccctgat
      541 cccgggtgat ggcatcggcc cggaagtcac cgatgccacg gtacaagtcc tcaatgccct
      601 gggatcgccc tttgaatggg atacacagat ggccggcatg gcggccatgg acgccagcgg
      661 cgatccattg ccagcggcgg tgctggacag catccgccgc acgcgcctgg cgctcaaggg
      721 cccgctgacc acgccgattg gcggagggtt ccggtcggtc aacgtgcgcc tgcgcgaaga
      781 attcaagctg tacgcgaacc tgcgcccggt caggacgatc atcccgggcc gctacgacaa
      841 catcgacatc gtgctggtgc gcgagaacat cgaaggcttg tatgtcgcgc atgaatactt
      901 cgtcccggtc ggcgacgatc cgcgcgcagt cgcggtgtcc accggtgtga acacgcgcga
      961 cggctgtcgt cgcatcgccc gctacgcgtt cgagtatgcg gtgaagaacg ggcgcaagaa
     1021 gatcaccatc gtgcacaagg cgaatgtgct caaggcgctg accggcatct tccttgaggc
     1081 ggcgcgcgag gtggcgcagg agtatgcggg ccgcgtggaa gtcgacgacc gtatcgtcga
     1141 tgcgtgcgcg atgcagctgg tgctcaatcc ctggcaattc gacatgctgc tctgtaccaa
     1201 cctgttcggc gacatcctgt ccgatcagct ggccgggctg gtgggagggc tgggtttggc
     1261 tcccggtgcg aacatcggcg agcaggcggc catcttcgag gccgtgcacg gctcggcgcc
     1321 agatatcgcc ggaaaaggct tggccaatcc gatctcgctg atgctggccg ctgcgctgat
     1381 gctggaccat gtcggcaaac cggagctcgc cacacgtctg cgcgatgcga tacaggcgac
     1441 gctgcttgag gacaatgtgc gcacccgtga tctggggggg acggcgtcga cggcacagtt
     1501 cacggcggcg ctgctcaagc gtctctgacc gggggtgcgg cacatggggg ccgccgagcg
     1561 ctccggcggg cccgcccccc ggtgttccgc cctaggtgag atgtgcgtag atgcggtagg
     1621 cgacatccgc gtcgctcaag tagccacgga tgccgtgccg gttctcggtg aaattatcga
     1681 catccgtctt gttctcgata gctggattga ccatgaagcg ctccccatcg aggggccaga
     1741 gagcgacgac atcacgcgga tccaatgcgt tgaaccagtc ccgtacgcat tcaggatggc
     1801 gcggcgcggg caccaatctc ctgcggatgg cgctgacggc gagcggtgaa cccagcgtaa
     1861 caaacagtgg tacgcgccag ccagcctgcg gcccgtagcg gtgcaacacg ttgtacgcaa
     1921 cgaccgaccc cagggaatga ccgaccacga ccgtgtccgt gtcccgggcg aacgcctggc
     1981 gcacaccctc gtcgatcaca tgccggatct cgttcgcatg gagataccgg aatacatcgt
     2041 tcgcaaacag cgcgacgctt gctttgctcg ccggcgatac gccatcaagc attctgagaa
     2101 tggcctgcac ccacggccag ttgagaaccc ctcgttctcc gtactcgaca tccggcagtg
     2161 cggcaggtag aggagaaccg atcgcttcgc acttttcctg gacctcgccc acgtactcct
     2221 gaatgaacga tttttcatcg tcgccgatgt caaggccgcg cacgacgact tcggcccgtt
     2281 cgccatcgct tacgccttgt accagctgat cgagcgtatc gccgtagtac ggaaatcgga
     2341 tctgggacgg cgatatcggc atcgtgaggc cgctcttggc caggccctcg ccccacgcat
     2401 cgatccactc ctgtttcagc gcgtttgcgt ctttgccgtg ctgcgccctg ccgtgaatga
     2461 acaccagttc tcgcatcgca tattctcctg ggccgcgggg tttcactgaa ggtcatggca
     2521 ccgtttgggg caaggcacga tcgatgcctt cttgcgtatc gccttcaccg tttcttgcgc
     2581 cctcgccagc aggcacgcgc gcatgcaaag cgggtaaacc ggcccgcctg tccagttcaa
     2641 gcagaagggt cgcagcggcg cgctgctcat ccggccttgc acgtttcccg tatttcgaca
     2701 acacgaacca gccgttgtcc ctgacggatt gccatctctc cagcgcatta tcgtattcgt
     2761 gcagttcact tagtctctgc atgatccttt ccagatttcc ggcgagttgc tgccgtacga
     2821 cgctttcgta gatgagcagc tcgactttta tcaggtgtga acgaaagtcg aggtaggtat
     2881 gaccggccgg gttcgtgccg catgacatgg ccttttgcgc aagcggagcg acagtctccc
     2941 agctcacggc ctcgccatga aggttgatct ctgcggcgag gaggttaagc gcgggatagt
     3001 gctgatccgc ggcctcgatc gcagcggcca gttcgagcgc ctttccgcat tgttccttca
     3061 tgttctcggt cgcatccttt tccttccaat cctggccggc gatcgcctcc accattgcaa
     3121 tgcgcttgca tatgtaggcg cacaggctgt gccgcttcgc agtggcctgg aacatgcaca
     3181 gtttctccaa cagcgccctt gccttgttca gttgcgccga cgcgctttca tgtgccctct
     3241 catacgcgcc gctgttgctg cggtcctccg ggccgtcacg accttccgga tcgggctcga
     3301 tatgggcgac tgcatcctgg aagactttcc aggcctgttg tccgtacacc ttcgcgagct
     3361 gttcccccgc tctcatcgat gcgctcgcgt caggggaagc aatcgcacgc tcgagatacg
     3421 tgcgggccgc attgaattca cccaccgccg cgtaggccag gccgaacgcc tctgcaacgg
     3481 atccaatgca ctcccactgt ttgccgtaat tttcggcaag gtagcgaatt ttcgccgact
     3541 gccgcgcggg gcttctcttc tcatacttcg cattggtcac gattgcctcc agcgcaatga
     3601 ccaaggcgga tgaagtcgat atgccggcaa actcctgggc gtgcggcggc tcacgtcttt
     3661 cgtcagcatc tctttcgttc tgcagccgcc agtccggatc tccatagcat tgatacgccg
     3721 cccaggttgt atttgtcatg ccgaacttgt cccacgcagc tttgcgcgcc tccgctaccg
     3781 cgtcgatgaa gcgcttcccg tccagaagcg ccttgtagaa cgtcgtggcg aactctctcg
     3841 ccgctctgtc ctcgactatc catccagccg cgacaacgca gcgcacgccg atcttgataa
     3901 ggcttttcgc gatatttgcc gcgaagcccg ctcgcccgta cgtcgacggc tgcggtgatg
     3961 cgagcagatc ggaagcatgc gccgccgcag agtggcagca gttgatgaac acgagctggg
     4021 gaacctgccg catgttcctg atctcgcgcg gcccgagata gatcccgctc gatagcacca
     4081 cccccctcgg atcgccatcc tggtcggcgg gctcaccgtg gcctgcaatg tggatgactc
     4141 gccagtcgcg tgacaagagc gtggtaatga ctctgcgcgc gtcgatctca tttgctccct
     4201 gtccgtcgct gacgagatcc atcacgcgtt cggcgcccaa tttttccttg atgcgcttgg
     4261 ctacggcctt tgcctcctcg cgtgcacccg gaaggcgcgg atagcgcagc gggtcacact
     4321 ttggctcccc gatcaccaac acatcagacg cggaatcggc atccatcact tcggaacgaa
     4381 agtccgccgt cagcagcctg cgaagcaatt tcacccgtat tgcccaaggc tccgcatcac
     4441 cttggtcctg ggcgcccgcc gatgtatcga gcagttccca cgggatgcat gccgtgccgt
     4501 cgtccagttc gatctgcact tccgtggtac ccccgagaaa tggctccatc tcggccggga
     4561 cgaccagctt gaacaaggct tgaccgattc tgttgtccag gaccgatttc ctggatgccg
     4621 ttgcgatcaa ctcctgcagc agtctgtcct gcgtatgttg ggcgcgaagc tctgcccgcg
     4681 ccctcctggt atcaagcgaa tattcgatga atctccggtc cggggcttcc acccgtgtca
     4741 atgcacgtat caggtcgtgg cgggcgccac gatagctgga atcaagcggg cgctgcatcg
     4801 cgccaacccc cgcctccaca gtcttttgca gcttgaactg ccccgggggc gcgccgccat
     4861 tttcaagaac ctcgagcgcc cgccaagctt cagtggcgcg gtccagatag atctcgatga
     4921 ttcgcaggtg cttgatgcag ggatagccca cctcgcacag ccggctgttc gcatccctga
     4981 ccccctgcgc aatggcctgt gcggcctgcc cggccgagac gcccgctccg ccgctcccga
     5041 gcaaggttgt cgtgagcgtc aatgactgtg gggcggtcgt ggccaatgcc acggtttccc
     5101 gtacccacgc aacgacgcct tgacgaactg tggccacatg ttccgggaca ctcaacttgc
     5161 cttcctgccc gagacccacc acgatgatcg tttccggctg ccgcggaagg taggcatcct
     5221 cacggcaaag gttgggattt ctaaacagtt gacgatcacc aggacgatgc gggtaaccgc
     5281 ctatgcgcag ggagttcgag agggcctccc catacatcag attgaggatg cgttcggttc
     5341 cggtgagtga ggtggaccgg tagtgtccaa ccatgagcgc tccgcggaca aaacgcaaat
     5401 tgccgttcat gacactgatg tctaacggct ccgactgcga ccccgcgatc aaagcattca
     5461 tgattccctc tccggcaggc ggacattgct cgggcatgga tgcatgcttc ttgcccgcac
     5521 cgacacaaga cgcgacagga cgactcgatc aaaccggccc ggacagcaca tagagcgaag
     5581 ttacggtacg cccggcggaa gggcacagct tgattccaca caaagcaagc ggagcttgtc
     5641 tttgcgcatc gcggctccga tccgtgtcga gtgcccgggg gtcaagcttg atttccggag
     5701 ccccgtttca tggatgttgc agagaagagg atcagatagg tattgggtga agactgcgct
     5761 acatcgccgg acggtcccat caatttcacc gcgtattcgg gggccgaaac cggattcagc
     5821 gctgagcgcg ggctgtcgga gagatacgat gaccgtgcga cgtaccagcg cgtgtcgccc
     5881 gatcggcagc ttcgtcgcgt tggggagcgc ggcagtacga cccggaacgc acgattcgtc
     5941 acggcacctc aagcgaattg agcgagtccg tgaccgaggg accaattttc ttttgcggcc
     6001 tcgagaacgt tcacgaagac gtcgtctggc cggatgcccg ggctttcgcc cggcagttcc
     6061 gcaaccctcc ggaacagcgc tttcttttgt tccgcggtgc gggtgttgaa cacggtgatc
     6121 tggatgttcg ggcttccaga tctgccgcgc ttgccggtgc tgcttcacac tcgagtcatg
     6181 gacggccggt cgcgcgacgc gcttgctgcg cttgcggccg ctttcagaag cgcagtgcgc
     6241 tagtcattca cattagcggc catggccgcg aatgctgatc attcaagcgc ctgctcctgc
     6301 cccgtcgctc ccgagcgggc aggagcccgg atcgatcgtc tgcagtcctt tgatagcctg
     6361 tgctggcccc ggcctgcgcg ggggcgacag gattgggtgt cgtcgcttga gcgtgcaagc
     6421 gcggggcggg atgaaatcga agcttgggat gcaatcccgg cacctgcctc cgctcgtccg
     6481 cccagcctcc acggcgcccc ttccctttcc cgccaaacga ctcaggggta ctcgacgttg
     6541 tggtagagca cgccgcttac cccggcgccg ccgagcggcg cccgcgagcc cacggtgatc
     6601 ggcgattcca gtgtgatcgc gttgccggag tcgttttcaa gcgtgatgcc ggtgagtccc
     6661 atgcgcagcg tgtagcgacc gcttgcattg gcattcgagg caccggcaag cacggcgcgg
     6721 tcggcatcgg ttgcggcggc gatggcggcc tgcatgatcg cgtcgagatt gaccagcagc
     6781 gcaaagctcg aggggtcgtg atcatgcact ggccgggtcg agacagtcac cgcgtccgcg
     6841 ggcacttcgg cgaccgtcac atttgcgctg gcgccgctgc cgttgttgac gaccacgtag
     6901 gcgcgtgccg tatcgggact ggcaatgctc atcctgcccg tctcgttgat ggtcagcgtc
     6961 gcaccttcga cgaggaactg gaacagctcg tcggaagccg tgttgtcgaa ctcgaccgcg
     7021 acgcggatcg tgccggactg gccgttcatg ccgatctggt ccacgcgcat gccgaaggtg
     7081 gacaacggcg tgcttgcacc ggctgcacgg gattcctgcg catacacgcc ggtcgtgctg
     7141 aacactgcgt aatcctgcgc attgacgtag tctccgccgt tgaacaggac ctggttgctg
     7201 cgcatcacga tggagccggc gaacgtgtcg tcggcgggcg cgggatcggt accaggatcc
     7261 gtgccagggt cggggtccgg atcggtaccc gggcctgtgc cgggaatgac tggagtgccg
     7321 ttgtccagcc cgggatcggc atcgtccggg ctcgagccgc cgcaggccgc cagggcgagc
     7381 agaacagccg tggcaagcgc cgcattcttc atcttcaaca tgagtttctc cgtgcgagtt
     7441 tgaaatgggg acggtagaga cggtgcatgc cggcttgacg gccggcatct gcattcacaa
     7501 ggcgccagat tagggcgttg acgcgcggcg acagttgcgg gacgtcaccg ccgcttccgc
     7561 gcttgcttgc gtcgcatcaa cggtgcttcc tcgcccggca tgccggccgg atgcggccgc
     7621 tcgaagatgg ggtgcggtgc gctggggtat agtggcgccc tcgcttgata cggcggcccg
     7681 catcttcgcc gccgccgacg tccgccaagc cgttcaccgc cagaacttcc acgccttgag
     7741 cacctacacg ctttccgccg ccgttgaatc ggccctcgat atccgc
//
LOCUS       DATLGF010000202         5058 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_68009,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000202 DATLGF010000000
VERSION     DATLGF010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68009"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VM406_06075"
     CDS             complement(<1..810)
                     /locus_tag="VM406_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL75563.1"
                     /translation="MTQLDHFLTRAEGLLARLEAYLPPPQREPDWQASSAFRWRKAGG
                     RDARGYLQPVKQLAPISLSDLQNIDAQKEQIEQNTRQFVEGRPANNVLLTGARGTGKS
                     SLIKACLNRFADHGLRLIEVDKDDLADLPDLVDMVAQRPERFVIFCDDLSFEEGEGGY
                     KALKVALDGSIAAQSDNVLIYATSNRRHLMPERMSDNLGYTHTEDGDLHPGETVEEKI
                     SLSERFGLWVSFYPFRQEHYLDIVAHWLAHFGCGTEQIEEARPEALRWALQR"
     gene            complement(826..2064)
                     /gene="argJ"
                     /locus_tag="VM406_06080"
     CDS             complement(826..2064)
                     /gene="argJ"
                     /locus_tag="VM406_06080"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HVL75564.1"
                     /translation="MAVNLPLPVAADLKPVAGIELGYAEAGVRKPNRKDVLVMRLAPT
                     ATVAGVFTKNRFCAAPVQVCKAHLEAVRLGSAPIRALLVNTGNANAGTGDAGLADANA
                     TCTALAELLGCEPAQILPFSTGVILEPLPVGRIKAGLPAAIANLKEDNWLAAAEAIMT
                     TDTQPKAASRTVTIGGKEVTMTGISKGAGMIKPNMATMLGYLAMDAKVAQPVLEHLVK
                     VAADRSFNSITIDGDTSTNDSFMLIATGAGELEIDDVESPDYAQLAEAVIGLSQQLAQ
                     MIVRDGEGATKFITIAVEQGASVEECRRIAYSIGHSPLVKTAFYASDPNLGRILAAIG
                     YAGVDDLDVGKLNLFLDDVWVAKNGGRNPDYKEEDGQRVMKQSEITIRVLLARGEAKA
                     TIWTCDFSHDYVSINADYRS"
     gene            complement(2179..4944)
                     /gene="secA"
                     /locus_tag="VM406_06085"
     CDS             complement(2179..4944)
                     /gene="secA"
                     /locus_tag="VM406_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HVL75565.1"
                     /translation="MSFLTKIFGSRNQRLIKQYQKTVREINALEPAMEKLSDAELQAK
                     TQQFRERLAGGATLDDLLVEAFAVCREAGKRVLKMRHFDVQLIGGIVLHQGKIAEMGT
                     GEGKTLMATLPVYLNALAGKGVHVVTVNDYLAQRDAEWMGRLYNWLGLSTGVNLSQMP
                     HGQKQTAYAADITYGTNNEFGFDYLRDNMVYDIGDRVQRGLNFAIVDEVDSILIDEAR
                     TPLIISGQAEDHTELYRKMNAVPPLLTRQIGEETKDGSGKIDVPGHYTLDEKNHQVLL
                     TEAGHEKAEEVLTEMGLLPEGASLYDAANITLVHHLYAALRAHTLYHKDQHYVVQNGE
                     VVIVDEFTGRLMSGRRWSEGLHQAVEAKEGVRIQNENQTLASITFQNYFRMYAKLGGM
                     TGTADTEAYEFQEIYGLETVVVPPNRPSQRKDRQDQVYRTAEEKYQAMLSDIQDCYER
                     GQPVLVGTTSIENSEQLSAILTQAKLPHNVLNAKQHAREAEIIAQAGRPKMITIATNM
                     AGRGTDIVLGGNVDKQIQLIEADPNLSDADKQARAKQLRDEWQSLHDHVVSVGGLHIV
                     GTERHESRRIDNQLRGRSGRQGDPGSSRFYLSLDDPLLRIFAGDRVRAIMDRLKMPEG
                     EPIEAGIVSRSIESAQRKVEARNFDIRKQLLEYDDVANDQRKVIYQQRNELLEAKEVS
                     ELVAGLRRGFFSDLFREHVPEGSMEEQWDIEGLESTLKNEWHLDVPLRQILETEQHLT
                     DEDLLERVLKAADDAYEAKVAVIGSEGFAGFERSVMLQSIDTHWREHLAALDHLRQGI
                     HLRGYAQKNPKQEYKREAFELFGQMLQMIKSEVVRIIMTVRIQTREEADAAEQQLAQS
                     QVSNVHYQHADFNPNAAPEELLAPTAEPADTRGQPMVHAVPKVGRNDPCPCGSGKKYK
                     QCHGKLA"
BASE COUNT          770 a         1613 c         1633 g         1042 t
ORIGIN      
        1 gcgctgcagc gcccagcgca gcgcttccgg ccgggcctct tcgatctgtt cggtgccgca
       61 gccgaagtgg gcaagccagt gcgcgacgat gtccaggtaa tgctcctggc ggaacggata
      121 gaacgacacc cacaatccga agcgctccga cagcgagatt ttttcctcga ccgtctcgcc
      181 gggatgcagg tcgccgtcct cggtatgggt gtagccgagg ttgtccgaca tgcgctccgg
      241 catcaggtgg cgccggttgg aggtcgcata gatcagcacg ttgtccgact gcgcggcgat
      301 gctgccgtcc agcgcgacct tcagcgcctt gtagccgccc tcgccctcct cgaacgagag
      361 gtcgtcgcag aagatgacga agcgctcggg acgctgggcc accatgtcga ccaggtccgg
      421 caggtcggcc agatcgtcct tgtcgacctc gatcaggcgc agcccgtggt ctgcgaaccg
      481 gttcaggcat gccttgatca gcgaggactt gccggtgccg cgcgcgccgg tcagcaacac
      541 gttgttggcg ggccgtccct cgacgaactg gcgcgtgttc tgttcgatct gctccttctg
      601 cgcatcgatg ttctgcaggt ccgacaacga tatcggcgca agctgcttga ccggctgcag
      661 atagccgcgc gcgtcgcgtc cgccggcctt gcgccagcgg aaagcggatg aggcctgcca
      721 gtcgggctct cgctgcggcg gcggaagata ggcttcgagg cgcgcgagca ggccctcggc
      781 gcgcgtgagg aaatggtcga gctgggtcat gaagcgggct tgcgattacg agcggtagtc
      841 ggcattgatg ctgacgtagt catgcgagaa atcacaggtc cagatcgtgg ccttggcctc
      901 gccgcgcgcg agcaggacgc ggatcgtgat ctcgctctgc ttcatcaccc gctggccgtc
      961 ttcttccttg tagtccgggt tgcgcccgcc gttcttcgcg acccagacat cgtcgaggaa
     1021 caggttgagc ttgccgacat cgagatcgtc caccccggcg tatccaatcg cggcgaggat
     1081 gcggcccagg ttcgggtccg acgcgtagaa cgcggtcttc accagcggcg aatggccgat
     1141 cgaataggcg atgcgacggc actcttccac gctcgcaccc tgctcgacgg cgatcgtgat
     1201 gaacttcgtc gccccctcgc cgtcgcgcac gatcatctgc gccagttgct gggacagacc
     1261 gatcaccgcc tcggccagct gcgcgtagtc gggcgattcg acatcgtcga tctccagttc
     1321 gccggcgccg gtcgcgatca gcatgaagga gtcattggtc gaagtgtcgc cgtcgatggt
     1381 gatgctgttg aacgaccggt ccgccgccac cttcacgagg tgttccagca ccggctgcgc
     1441 gaccttcgca tccatcgcaa ggtagccgag catcgtcgcc atgttcggct tgatcatgcc
     1501 ggcacccttg ctgatgccgg tcatcgtgac ctctttgccg ccgatggtca ccgtgcgcga
     1561 tgccgccttc ggctgggtat cggtggtcat gatcgcctcg gcggcggcca gccagttgtc
     1621 ttccttcagg ttggcaatcg ccgccggcag gccggccttg atgcgaccca ccggcagcgg
     1681 ctccaggatc acgccggtgg agaacggcag gatctgcgcc ggttcacagc cgagcagctc
     1741 ggccagcgcc gtgcaggtcg cattggcatc ggccaggccg gcgtcgccgg tgccggcatt
     1801 ggcgttgccg gtattgacca gcagcgcgcg gatcggcgcg ctgcccagcc gcacggcctc
     1861 caggtgcgcc ttgcatacct gcaccggcgc cgcgcagaag cggttcttcg tgaagacgcc
     1921 ggcgaccgtc gcggtcgggg cgagtcgcat caccagcacg tccttgcgat tgggcttgcg
     1981 cacgccggct tcggcatagc cgagctcgat gccggcaacc ggcttcaggt ctgctgcaac
     2041 gggaagggga agattgacgg ccatggaagc aatgtaagtg agagggaaag ccgactattc
     2101 tatcggatcg gcgcaataaa aaagggcagg tttccctgcc ctttccctgc ccgtgttccg
     2161 atcggatcgg atcggcgctt acgccagctt gccgtggcac tgcttgtact tctttccgct
     2221 gccgcagggg cagggatcgt tgcgccccac cttcgggacc gcatggacca ttggctggcc
     2281 gcgcgtgtcc gccggttcgg cggtcggcgc aagcagttcc tcgggtgcgg cgttcggatt
     2341 gaagtccgcg tgctggtagt ggacattcga gacctgcgac tgcgcgagct gctgttctgc
     2401 agcatcggct tcctcgcggg tctggatgcg taccgtcatg atgatgcgca ccacctcgct
     2461 cttgatcatc tgcagcattt gaccgaacag ctcgaatgcc tcgcgcttgt actcctgctt
     2521 cggattcttc tgcgcatagc cgcgcaggtg tatgccctgg cgcaggtggt cgagcgccgc
     2581 gaggtgctcg cgccagtggg tgtcgatgct ctgcagcatg acgctgcgct cgaagccggc
     2641 gaagccttcg cttccaatca ccgcgacctt tgcctcgtag gcgtcgtcgg ctgccttgag
     2701 cacgcgctcg agcagatcct cgtcggtcag gtgctgttcg gtctcgagta tctgcctgag
     2761 cggcacgtcg agatgccact cgttcttcag cgtgctttcc agtccttcga tgtcccactg
     2821 ctcttccatc gagccttcgg gcacatgctc gcggaacagg tccgagaaga agccgcggcg
     2881 cagcccggcc accagttccg acacttcctt cgcctccagc agctcgttgc gctgctggta
     2941 gatcaccttg cgctggtcgt tggcgacatc gtcgtattcg agcagctgct tgcggatgtc
     3001 gaagttgcgc gcctcgacct tgcgctgcgc cgattcgatc gagcgcgaaa caatgccggc
     3061 ctcgatcggc tcgccctcgg gcatcttcaa ccgatccatg atcgcgcgca cgcggtcgcc
     3121 ggcaaagatg cgcaagagcg ggtcgtcgag cgacaggtag aagcgcgagg agcccggatc
     3181 gccctggcgc ccggagcggc cgcgcagctg attgtcgatg cggcgcgatt cgtggcgctc
     3241 ggtgccgacg atgtgcaaac cgccgaccga caccacgtgg tcatgcagcg actgccattc
     3301 gtcgcgcagc tgcttggccc tggcctgctt gtccgcgtcg gagagattgg ggtcggcctc
     3361 gatcagctgg atctgcttgt cgacattgcc gcccaggacg atgtcggtgc cgcgaccggc
     3421 catgttggtc gcgatcgtga tcatcttcgg ccggccggcc tgggcgatga tctccgcctc
     3481 gcgggcgtgc tgcttcgcgt tgagcacgtt atgcggcagc ttggcctggg tcaggatggc
     3541 ggagagctgt tcggagttct cgatggaggt cgtgccgacc agcaccggct ggccgcgctc
     3601 gtaacaatcc tgaatgtcgc tgagcatcgc ctggtatttc tcctcggcgg tgcggtagac
     3661 ctgatcctgg cgatccttgc gctggctggg acggttgggc ggcaccacca cggtttccag
     3721 tccgtagatt tcctggaatt cgtaggcttc ggtgtcggcc gtgccggtca tgccgcccag
     3781 cttcgcatac atgcggaagt agttctggaa ggtgatcgag gccagcgtct ggttctcgtt
     3841 ctggatgcgc acgccttcct tcgcctcgac tgcctggtgc aagccttccg accagcggcg
     3901 cccgctcatc aggcggccgg tgaattcgtc gacaatcacg acctcgccgt tctgcaccac
     3961 gtaatgctgg tccttgtggt acagcgtatg cgcacgcaat gctgcataga ggtggtgcac
     4021 cagcgtgatg ttggctgcgt cgtacaggga tgcgccctcg ggcagcaggc ccatttcggt
     4081 gagaacctcc tcggcctttt cgtgaccggc ttcggtcagc agcacctggt ggttcttctc
     4141 gtccagcgtg tagtggcccg gcacgtcgat cttgccgctg ccgtccttgg tctcttcgcc
     4201 gatctgacgc gtcaacagcg gcggcaccgc attcatcttg cggtagagct cggtatggtc
     4261 ttcggcctgg ccggagatga tcagcggggt gcgcgcctcg tcgatcagga tcgagtccac
     4321 ttcgtcgacg atcgcaaagt tcaatccgcg ctgcacacgg tcgccaatgt cgtagaccat
     4381 gttgtcgcgc agatagtcga agccgaactc gttattggtg ccgtaggtga tgtcggccgc
     4441 gtaggcggtc tgcttctggc cgtgcggcat ctgagacagg ttcacgccgg tcgagaggcc
     4501 gagccagttg tacaggcgcc ccatccactc ggcatcgcgc tgcgcgaggt aatcattgac
     4561 cgtgaccacg tggacgccct tgccggccag cgcgttcagg tagaccggca gcgttgccat
     4621 cagcgtcttg ccttcgccgg tacccatttc ggcaatcttg ccctggtgca gcacgatccc
     4681 gccgatcagc tggacgtcga agtgacgcat cttcaggacc cgcttgccgg cttcgcggca
     4741 gacggcgaac gcttccacta gaaggtcgtc gagcgttgcg cctccggcca ggcgctccct
     4801 gaactgctgc gtcttggcct gcagttcggc gtcggacagc ttttccattg ccggttcgag
     4861 cgcgttgatc tcgcggaccg ttttttggta ttgcttgatc agccgctggt tgcgactgcc
     4921 gaaaatcttg gtcaggaatg acatgctagg aatcagtgag ctgccgtggc gagccggcaa
     4981 actgccgctc tcgcgtccgg cctgggacgt tggtcaaatg gcggatttta tcatgcgccc
     5041 cggactgccc cgggtcgc
//
LOCUS       DATLGF010000203         3426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_68023,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000203 DATLGF010000000
VERSION     DATLGF010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68023"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VM406_06090"
     CDS             <1..737
                     /locus_tag="VM406_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HVL75566.1"
                     /translation="FVPQCGLAPGFISIVGHDVASRFDSLRDVHMRVGALPTFPSNAL
                     KYNLTWSTDGLINEYCNPCEAIVDGRLREVPALEEVEHFSLDGIDYEAFNTSGGLGTL
                     CETLAGRVQNLNYKTVRYPGHRDIIKMLVRDLQLGMLDRRPILKEVLETAIPITRQDV
                     VLVFASVCGTRDGRLEQETYARKIYSQEVNGTLLSAIQITTAAGICAMVDLVLEGKLP
                     GKGLVRQEQARLEDFLSNRFGRYYAG"
     gene            794..2296
                     /locus_tag="VM406_06095"
     CDS             794..2296
                     /locus_tag="VM406_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVL75567.1"
                     /translation="MEVVEKLLADIGADLSRYRGEDLISRSPITGERTAGLRADHAAD
                     VAVKVARAHAAFQQWRSVPAPRRGELVRLFGEELRENKEALGRLVTLEAGKILQEGLG
                     EVQEMIDICDFAVGLSRQLYGLTIASERPGHRMMETWHPLGVVGVISAFNFPVAVWSW
                     NAALAFVCGDSVVWKPSEKTPLTAIAVQALFEKAVARFGEAPEGLSELVIGGADAGQA
                     LVDDARVPLVSATGSTRMGRAVAQACAKRLARTILELGGNNAMIVAPSADLELATRAI
                     VFAAVGTAGQRCTTLRRLFVHDSVYDQLVPRLKKVYEGLPVGNPLEKGTLVGPLIDGA
                     AFDGMQRALARAAEEGGAVFDGGRAEVAGCEDAFYARPALVEMPAQTPIMHHETFAPI
                     LYVTRYQSLDQAIAWNNAVPQGLSSSIFTNDIREAEHFMAATGSDCGIANVNIGPSGA
                     EIGGAFGGEKETGGGRESGSDSWKAYMRRATNTVNYSRALPLAQGISFDF"
     gene            2478..>3426
                     /locus_tag="VM406_06100"
     CDS             2478..>3426
                     /locus_tag="VM406_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVL75568.1"
                     /translation="MTATRQAGHAADDSPDYSTNPVSRSTFDEVMVPVYAPSAMVPVR
                     ASGLDLWDQNGRQYLDFTAGIGVTGLGHCAPHLVSVMHEQAQRLWHAGNAFTNEPVLQ
                     LARAFTEATFAERVFVCNSGAEANEAAFKLARKYAHTVHNAGKSRIVSCFNAFHGRTL
                     FTVSVGGQSKYTEGFEPLPPAIEHIPFNDAASARAAIGDDVCAVVVEPVQGEGGVLPA
                     DPEWLRLLRTLCDQSGALLIFDEVQCGMGRTGKLFAYMDYGVTPDILTTAKALGNGYP
                     VAAMLTTEKIATVFSAGVHGTTYGGNPLAGRIALEVLRTI"
BASE COUNT          596 a         1106 c         1181 g          543 t
ORIGIN      
        1 ccttcgtgcc gcagtgcggt cttgcacccg gcttcatttc catcgtcggc cacgacgtgg
       61 ccagccgctt cgacagcctg cgcgacgtgc acatgcgggt aggtgcgctg ccgacctttc
      121 cgagcaacgc gctgaagtac aacctgacct ggagcaccga cggcctgatc aacgaatact
      181 gcaatccctg cgaggccatc gtcgacggcc ggctgcgcga agtgccggcg ctggaagagg
      241 tcgagcactt ctcgctcgac ggcatcgact acgaggcgtt caacacctcg ggcggcctcg
      301 gcacgctgtg cgagacactc gcaggacggg tgcaaaacct caactacaag accgtgcgct
      361 acccggggca cagggatatc atcaagatgc tcgtgcgcga cctccagctc ggcatgctgg
      421 accgccggcc gatcctgaag gaagtcctcg agaccgcgat tccgatcacg cgccaggacg
      481 tggtgctggt ctttgcaagc gtatgcggaa cgcgcgacgg caggctggag caggagacgt
      541 atgcacgcaa gatctacagc caggaagtca acggcacgct gctgtcggcg atacagatca
      601 cgaccgccgc aggcatctgc gcaatggtcg acctggtgct ggaaggcaag ctgcctggta
      661 aggggctggt acgccaggag caggccaggc tggaggactt cctgtccaat cgcttcggcc
      721 gctactatgc gggctgatac cgctgccagc ccgaaagcaa ccggatacca actacaagga
      781 agggacaaca tcgatggaag tcgtggaaaa gctgctcgcc gacatcggcg ccgacctgtc
      841 gcgttaccgc ggcgaagacc tgatcagccg cagcccgatc accggcgagc gcaccgccgg
      901 cctgcgcgcc gaccacgccg ccgatgtggc ggtgaaggtg gcgcgcgcgc atgcggcgtt
      961 ccagcagtgg cgcagcgtgc cggcgccgcg gcgcggcgag ctggtgcgcc tgttcggcga
     1021 ggagttgcga gaaaacaagg aggcgctcgg tcgcctggtc acgctggagg ccggcaagat
     1081 cctgcaggaa ggcctgggcg aggtgcagga gatgatcgac atctgcgact tcgcggtcgg
     1141 cctgtcgcgc cagctttacg ggctgacgat cgcctccgag cggcccggcc acaggatgat
     1201 ggagacttgg catccgctcg gcgtggtcgg cgtgatttcc gcgttcaatt tcccggtcgc
     1261 ggtatggtcg tggaacgcgg cgctcgcctt cgtctgcggc gacagcgtgg tgtggaagcc
     1321 atccgagaag acgccgctga cggcgattgc cgtgcaggcg ctgttcgaga aggcagtcgc
     1381 ccgtttcggc gaagcgccgg agggcctgtc ggaactggtg atcggcggcg ccgacgccgg
     1441 gcaggcgctg gtggacgatg cgcgcgtgcc gctggtgagc gccaccggca gcacccgcat
     1501 gggacgcgcg gttgcgcaag cctgcgcgaa gcggctcgcc cgcaccatcc tcgagctggg
     1561 cggcaacaat gcgatgatcg ttgcaccgtc ggccgacctc gaactggcca cgcgtgcgat
     1621 cgtgttcgcg gcggtcggca cggccggcca gcgttgcacg acgctgcgcc gcctgttcgt
     1681 gcacgacagc gtgtacgacc agctggtgcc gcggctgaag aaggtgtacg agggactgcc
     1741 ggtcggcaat ccgctggaga agggaacgct ggtcggcccg ctgatcgacg gcgcggcctt
     1801 cgacggcatg cagcgcgcgc tcgcccgcgc ggcggaagag ggcggcgccg tgttcgacgg
     1861 cggccgtgca gaggtcgcgg gttgcgagga cgcgttctat gcgcgtccgg cgttggtcga
     1921 gatgccggcg cagacgccga tcatgcacca cgagaccttc gcaccgatcc tgtacgtgac
     1981 gcgctaccag tcgctcgacc aggcgattgc ctggaacaac gcggtgccgc aagggctttc
     2041 gtccagcatc ttcaccaacg acatccgcga agccgagcat ttcatggcgg ccaccggcag
     2101 cgactgcggc atcgccaatg tcaacatcgg cccgagcggc gccgagatcg gcggcgcgtt
     2161 cggcggcgag aaggaaaccg gcggcgggcg agaatcggga tccgattcgt ggaaggccta
     2221 tatgcgccgc gcgaccaaca cggtgaacta cagccgggcg ttgccgctgg cgcagggcat
     2281 cagcttcgat ttctgaaccg acgtcacgcg gaagccgccc gggttcgctt caagtcagtt
     2341 cgcgcgtgcg gtgcgcgccg acgcgcggtt ttcctcggca cgcccccgcg ccgcgcactt
     2401 tttcaggaag tgggcgtcgc agttagaaga gcgccgcgat gcgcagcgtc ccggcatcgt
     2461 aatgactagg agcaagaatg accgcgacaa gacaggcggg tcacgccgcc gacgactctc
     2521 ccgattactc gacgaatccg gtctcgcgca gcaccttcga cgaggtcatg gtgcccgtct
     2581 atgcgccgag cgcgatggtg ccggtgcgcg cgagcggtct cgacttgtgg gaccagaacg
     2641 gccgccagta tctggacttc accgcaggga tcggtgtgac cggtctgggc cactgtgcgc
     2701 cgcatctcgt ttccgtcatg catgaacagg cccagcggct ctggcatgcg ggcaatgcgt
     2761 tcaccaatga accggtgctg caactggcgc gcgcgttcac cgaggcgacc ttcgccgagc
     2821 gggtgtttgt ctgcaactcg ggcgcggaag cgaacgaagc ggcgttcaag ctggcgcgca
     2881 agtacgcgca caccgtgcac aacgccggca agtcgcgcat cgtgtcctgc ttcaatgcgt
     2941 ttcacggacg cacgctgttt accgtgtcgg tgggcggaca gtcgaagtac accgagggct
     3001 tcgagccgct gccgccggcg atcgagcaca tcccgttcaa cgacgctgcc tccgcccgcg
     3061 ccgcaatcgg cgacgacgtc tgcgcggtcg tggtcgagcc tgtccagggc gagggcgggg
     3121 tgctgccggc cgatcccgaa tggctgcggc tgctgcgcac gctgtgcgat cagagcggcg
     3181 cgctgctgat cttcgacgag gtgcagtgcg ggatgggccg caccggcaag ctgttcgcgt
     3241 acatggacta cggcgtcaca cccgacatcc tcactaccgc caaggcgctc ggcaacggct
     3301 acccggtggc cgcgatgctc acaaccgaaa agatcgccac cgtgttttca gccggcgtgc
     3361 atggcaccac ctatggcggc aatccgctcg ccgggcgcat tgcgcttgag gtgctgcgca
     3421 cgatag
//
LOCUS       DATLGF010000204         3146 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_87027,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000204 DATLGF010000000
VERSION     DATLGF010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87027"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="VM406_06105"
     CDS             complement(<1..1191)
                     /locus_tag="VM406_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVL75569.1"
                     /translation="MYTRHFHLQQPPFSIAPDPRYLFMSERHREALAHLLYGIDGGGG
                     IVLLSGEIGTGKTTLCRCLLEQVPANCNIAYVFNPKLSATELLEAICAEFRVTLAPNE
                     RRTGTRAWTNALNRFLLEQHALGRNNVLVIDEAQNLSADVLEQLRLLTNLETNQRKLL
                     QIILIGQPELRTMLAQPRLEQLAQRVIAHYHLGPLNEQETASYVQHRMTTAGLHGPSP
                     FQPGVMRHVHRLSGGVPRRINLLCDRALLGAYAEGVTTVSRRIVEHAASELFIHASRP
                     SPPSRRAPMFAFGAAAGILLAAGAAWLLMPELAARMPRGAPGVAVAAPQPAAEPAAAV
                     EEKQAVAQESAAVTPPFAGIRNETQALRELAALWGWQDEGGDPCAGNTQGLRCYRSSG
                     GLAEL"
     gene            1311..2096
                     /locus_tag="VM406_06110"
     CDS             1311..2096
                     /locus_tag="VM406_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVL75570.1"
                     /translation="MPLDPATFTALRCRDCRNGAGLDFAFSMAFQPIVDVRRRRIHAY
                     EALVRGPANEGAATILGRVHDGNRYRFDQACRVKAIEVAARLGLPEREGCLLHINFLP
                     NAVYRPETCIRATLEAAAEFRFPTERIVFEVTEGEKVRDEAHLQGIVTEYRRHGFATA
                     IDDFGAGYSGLNLLANFQPDIIKLDMALTRNLDMDRARRAIVAGVLMTCRELGIVVIA
                     EGVETAGELRALEDLGVELVQGYLLGKPAFEQLLPIQTDWEKT"
     gene            2093..2605
                     /locus_tag="VM406_06115"
     CDS             2093..2605
                     /locus_tag="VM406_06115"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HVL75571.1"
                     /translation="MKRKTALPFLLGAMISASAMAEPQMKPGLWEMTVKSDAMKNMPQ
                     MPPEQVEQMRKMGIEVPQFKNGAMVSRVCITPQMAAQGAAGFEKYDSGCVAKNRQVSG
                     NGYKADIVCTGAALNGSGKVSGTYSGGDKFSSSYDFKGTSHGQPVSHRQDSAGRWLGA
                     DCGSVKPFGQ"
     gene            complement(2607..>3146)
                     /locus_tag="VM406_06120"
     CDS             complement(2607..>3146)
                     /locus_tag="VM406_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75572.1"
                     /translation="LPGTLPNPGVAPMPGVVPSSGVVPSPGVSPVVGLPNPVPMLGTT
                     PTIGTGLGSPTTGLTPGLGTTPELGTTPGIGATPGFGSVPGSSIGITPGIGGTVGIGG
                     TTGIGVTGMDPGSGIDTTTVTTTGTGLSGSLPGSQPPLGTTTGAFGGTLTQSPLTGTG
                     LGTGTSGSTMLQGTGSTGF"
BASE COUNT          520 a         1033 c         1061 g          532 t
ORIGIN      
        1 cagttcggcc agcccgccgc tgctgcggta gcagcgaagg ccctgcgtgt tcccggcgca
       61 gggatcgccg ccctcatcct gccagcccca cagcgccgcc agttcgcgca gggcttgcgt
      121 ttcgttgcgg atgccggcaa acggcggcgt gacggctgcg ctttcctgtg cgacggcctg
      181 tttttcctcg accgcggcgg ccggttccgc ggccggctgc ggcgccgcga cggccacacc
      241 cggtgcaccc cgcggcatgc gcgcggcaag ttccggcatc agcagccagg cggcgccggc
      301 cgcaagcaga atgcccgccg ccgcgccgaa ggcgaacatg ggcgcgcgcc gcgaaggcgg
      361 cgaaggccgg ctggcgtgaa tgaacagttc ggacgccgca tgttcgacga tcctgcggct
      421 gaccgtcgtc acaccctcgg cgtaggcgcc gagcagggcg cggtcgcaca ggaggttgat
      481 ccggcgcggc accccgccgg acaggcggtg gacatgccgc atcacgccgg gctggaacgg
      541 cgaggggccg tgcaagccgg cggtggtcat ccggtgctgg acatagctcg cggtctcctg
      601 ctcgttgagc gggcccagat ggtaatgcgc gatcacccgc tgggccagct gctcgagccg
      661 gggctgggcc agcatcgtgc gcagctcggg ctggccgatc aggatgatct gcagcagctt
      721 gcgttgattg gtctccaggt tggtcaagag gcgcagctgc tcgagcacgt cggccgacaa
      781 attctgcgcc tcgtcgatca ccagcacgtt gttgcgccct agcgcgtgct gttcgaggag
      841 aaagcggttc agcgcattgg tccaggcgcg cgtgccggtg cgccgttcgt tcggcgcaag
      901 cgtcacccgg aattcggcgc agatcgcttc cagcaactcc gtggcgctca gcttcggatt
      961 gaagacgtag gcaatgttgc agttggccgg cacctgctcc agcaggcaac ggcacagcgt
     1021 ggtctttccg gtgccgattt cgcccgaaag cagcacgatg ccgccgccgc cgtcgatgcc
     1081 gtacaacagg tgggcaagcg cctcgcggtg gcgttcgctc atgaacaggt agcgcgggtc
     1141 gggcgcgatc gagaagggcg gctgctgcag gtggaagtga cgcgtgtaca tggcgccgat
     1201 tgtaatgcca cgcggactga tgcctgggtg catgcgcttg atacagcgca agccgcaccc
     1261 gggccaggga tgccgtagaa tcgattaccc gtcatcaagc gccaacctgc atgcctctcg
     1321 atcccgccac atttaccgcg ctgcgttgtc gcgattgccg caacggtgct ggcctggact
     1381 tcgcgttctc gatggcgttt cagcccatcg tcgatgtgcg ccgccgccgt atccatgcct
     1441 acgaggcact ggtccggggg ccggctaacg agggcgcggc caccattctg ggccgggtcc
     1501 atgatggcaa tcgctaccgc tttgaccagg cctgcagggt caaggccatc gaggtggctg
     1561 cacgcctcgg cctgcccgaa cgcgaaggct gtctgcttca catcaatttt cttccgaacg
     1621 ccgtctaccg gcccgaaacc tgcatccgtg ccacgcttga ggctgccgcc gagttccgct
     1681 ttcctaccga gcgcatcgtt ttcgaggtga ccgagggtga gaaggtgcgg gacgaggcgc
     1741 acctgcaagg gatcgtcacc gaatatcgac gccatggctt tgccactgct atcgacgatt
     1801 tcggcgccgg ctattcgggg ctgaacctgc tggcaaactt ccagcccgac atcatcaagc
     1861 tggacatggc actgacccgc aacctcgaca tggaccgcgc gcgccgcgcg attgtcgctg
     1921 gcgtactcat gacgtgccgg gagctcggca tcgtggtcat tgcagagggc gtggagaccg
     1981 caggcgaact gcgcgcactg gaggatttgg gcgtcgagct ggtgcagggg tatctgctcg
     2041 gcaaacccgc attcgagcag ctgcttccga ttcaaaccga ctgggagaaa acatgaaacg
     2101 aaaaactgcc cttccgttcc ttcttggcgc gatgatcagc gcaagcgcca tggccgaacc
     2161 gcagatgaag ccgggcctgt gggaaatgac ggtcaagtcc gacgccatga agaacatgcc
     2221 gcaaatgccg cccgagcagg tcgagcagat gcgcaagatg ggcatcgagg tgccgcagtt
     2281 caagaacggt gcaatggtga gccgggtttg catcacgccc cagatggcag cgcagggcgc
     2341 ggcgggattc gagaaatatg actccggctg cgtcgcgaag aaccgccagg tcagcggcaa
     2401 cggctacaag gccgacatcg tctgcaccgg cgccgcgctg aatggctccg gcaaggtgtc
     2461 gggcacctat tcgggcggag acaagttcag ctcttcatac gacttcaagg gcacctcgca
     2521 cggccagccg gtctcgcacc gtcaggacag cgcgggccgc tggctcggcg ccgactgcgg
     2581 cagtgtgaag ccgttcggcc agtagatcag aaacccgtag accctgttcc ctgcagcatc
     2641 gtcgatccgc tggtccccgt gcccagcccg gttcccgtga gcggggactg ggtcagcgtg
     2701 ccgccgaacg cgccggtcgt cgtgcccagc ggcggctgcg accccggcag gctgccgctc
     2761 aggccggttc cggtggtcgt cacggtagtc gtatcgattc cgctgcccgg gtccatgccg
     2821 gtgacgccga tcccggtcgt tccgccgatc ccgaccgtcc cgcctatgcc tggcgtgatc
     2881 ccgatgctgc tgccgggtac gctgccaaac cccggcgtcg cgccaatgcc gggcgtggtt
     2941 cccagttccg gcgttgtccc cagtcccgga gtcagtccgg tcgtcgggct acccaatccg
     3001 gtgccgatgg tcggcgtcgt gccgagcatc ggcaccggat tgggtagccc gacgaccgga
     3061 ctgactccgg gactggggac aacgccggaa ctgggaacca cgcccggcat tggcgcgacg
     3121 ccggggtttg gcagcgtacc cggcag
//
LOCUS       DATLGF010000205         5158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_4234,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000205 DATLGF010000000
VERSION     DATLGF010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5158
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4234"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VM406_06125"
     CDS             <1..428
                     /locus_tag="VM406_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-associated protein 3"
                     /protein_id="HVL75573.1"
                     /translation="SAQFSITGTPAAGDTFVAEPVRSESIFNTLDNLIAALERPATDA
                     NGKAALENSLRAANRELGSALDNVLTVRASVGARLNELDALDDQAADRDIQLAKTLTE
                     LQELDYTKAITDLTKQKMILEAAQQSFITTSRLSLFNFI"
     gene            complement(503..1288)
                     /gene="fliR"
                     /locus_tag="VM406_06130"
     CDS             complement(503..1288)
                     /gene="fliR"
                     /locus_tag="VM406_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HVL75574.1"
                     /translation="MITFSSTELYTWVASFLWPATRVLGLIATAPLFGNVSVPVRVKV
                     GLGIMISLVIAPGVPEVPAMDPMSLAGLVILAQQLVIGLAMGFAMRIVLAAVEMAGEI
                     SGLTMGFGFATFFDPQTRARTSVIAQFLSLLTLMIFLALNVHLMMLSTLAHSFVTLPI
                     SADPVGVSGFGQIARWGGLIFSSGVQLALPMIAALLLTNIALGILTRAAPQLNLFGIG
                     FPVTVGAGFIVLGLVLPFLATPIGRLFEEALGFIRQITTPLGG"
     gene            complement(1319..1588)
                     /gene="fliQ"
                     /locus_tag="VM406_06135"
     CDS             complement(1319..1588)
                     /gene="fliQ"
                     /locus_tag="VM406_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HVL75575.1"
                     /translation="MSPESVMTIGRQAIEVTLMVAAPMLLTALAVGLVVSIFQAATQI
                     NEMTLSFIPKLIGIFLAMMIAGPWMLSVMANYMRHMLTGIPGFIG"
     gene            complement(1599..2345)
                     /gene="fliP"
                     /locus_tag="VM406_06140"
     CDS             complement(1599..2345)
                     /gene="fliP"
                     /locus_tag="VM406_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882590.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HVL75576.1"
                     /translation="MGFKTFRFLLPLALLGLPLFASAQTGLPAFTSTPGPGGAQTYTL
                     SLQTLLLLTALTMLPAALLMMTSFTRIIIVLSLLRQALGTQTAPPNQVLIGLALFLTL
                     FVMGPVFDRIYAEAYQPLAENKITMSDALERGAVPLKDFMLRQTRESDLALYVRLSGG
                     PALNGPEDVPLRVLIPAFITSELKTAFQIAFAIFIPFLIIDMVVASILMSMGMMMMSP
                     AIVSLPFKLMLFVLVDGWQLLIGSLAQSFY"
     gene            complement(2405..2812)
                     /gene="fliO"
                     /locus_tag="VM406_06145"
     CDS             complement(2405..2812)
                     /gene="fliO"
                     /locus_tag="VM406_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HVL75577.1"
                     /translation="MKHALPLFLLAGAAPAFGADAPAGTAGSLLQVLFGLVVVLALMA
                     GAAWLLRRMGVAGTGTANVARVVGGVSVGNRERVMVVEVADQWIVVGVAPGRVNALSS
                     LPRQEIIPTHPAPAQSSEFSAWLRKTIEKRNAG"
     gene            complement(2823..3239)
                     /gene="fliN"
                     /locus_tag="VM406_06150"
     CDS             complement(2823..3239)
                     /gene="fliN"
                     /locus_tag="VM406_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HVL75578.1"
                     /translation="MADENGSQTAADDDWGAAFAEQQQAEQAALQSKQTAAHMFQDLS
                     GTVRTGTHNDIDFILDIPVQLTVELGRTKIAIKNLLQLAQGSVVELDGLAGEPMDVLV
                     NGCLIAQGEVVVVNDKFGIRLTDIITPSERIRKLNR"
     gene            complement(3232..4233)
                     /gene="fliM"
                     /locus_tag="VM406_06155"
     CDS             complement(3232..4233)
                     /gene="fliM"
                     /locus_tag="VM406_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HVL75579.1"
                     /translation="MSDSFLSQEEVDALLKGVTGEEDDHAAAEDESGIRPYNLATQER
                     IVRGRMPTLEIINERFARLLRIGLFNFLHRSAEVSIGPVRVHKYSEFIRNLVVPTNLN
                     LVHMKPLRGTALIVFDPNLVFLLVDNLFGGDGRFHTRVEGRDFTQTEQRIIQRVLGIV
                     FETYAKSWEPVYPVEFEYVRSEMNTQFANIATPNEVVVATTFTIELGPVSGEMHFCTP
                     YAMVEPIRDILTSSLQGETLETDKRWVRLMTQQIQTAEVELVADLGTAKATLGEVLNL
                     KAGDVLPITIPEKVAAKVDGVPVMECSYGKFNGQYALRVEKLLTYSTNDFTQGDDHG"
     gene            complement(4238..4750)
                     /gene="fliL"
                     /locus_tag="VM406_06160"
     CDS             complement(4238..4750)
                     /gene="fliL"
                     /locus_tag="VM406_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HVL75580.1"
                     /translation="MATASKAGPKVVQIDDGAKAPPPKKSPVKLLVVAAVLLMGAGGA
                     GAWFFMGAKAEKEAAAKAETPKPPLYLPIDQFTVNLQQEMGEHYLQTAFTLQVSDQSQ
                     ADLIKLYMPLVRSRLLMLLSSKKPSELSTAEGKEKLQEEIVARLQEPFAPNGAPVGVT
                     GVFFTSFVIQ"
BASE COUNT          935 a         1668 c         1653 g          902 t
ORIGIN      
        1 gctccgcaca gttctcgatc accggcacgc cggcggccgg cgacaccttc gtggccgaac
       61 cggtgcgcag cgagagcatc ttcaataccc tcgacaacct gatcgccgcg ctggaacgtc
      121 cggcgacgga cgcgaacggc aaggctgcgc tggagaactc cctgcgcgcg gcgaaccggg
      181 agctcggcag cgcgctcgac aatgtgctga cggtgcgcgc gtcggtcggc gcgcggctga
      241 acgaactcga cgcgctcgac gaccaggccg cggaccgcga catccagctc gccaagaccc
      301 tgaccgagct gcaggagctc gactacacga aggcgatcac cgacctcacg aagcagaaga
      361 tgattttgga ggccgcgcag cagtccttca tcacgacctc gcggctgtcg ctgttcaact
      421 tcatctgatc ggacgatacc ggcggttcca tcggcgtcgt ccaaggctgt cgccgcttct
      481 gcagtgccgc cccggcgaat ccctaccccc caagcggcgt cgtgatctgc cggatgaatc
      541 ccagcgcctc ctcgaacagc cggccgatcg gggtggccag gaagggcaga acgagaccca
      601 acacgatgaa acccgcgccg accgtgactg gaaagccgat gccgaacagg ttcagctgcg
      661 gcgccgcgcg cgtgagtatg cccagagcga tgttggtcag cagcagcgcg gcgatcatcg
      721 gcagcgcgag ctgcacaccg gagctgaaga tcaatccgcc ccagcgcgca atctgaccaa
      781 agcccgagac gcccaccgga tcggcgctga tcggcagcgt cacgaaactg tgcgcgagcg
      841 tggacagcat catcaggtgc acgttcagcg ccaggaagat catcagcgtc aacagcgaca
      901 ggaactgcgc gatgaccgac gtgcgcgcgc gcgtctgcgg atcgaagaag gtggcgaagc
      961 cgaaacccat cgtcaggccg ctgatttcgc ccgccatctc cactgccgcc agcacgatgc
     1021 gcatcgcaaa gcccatcgcc agcccgatca ccagctgctg ggccaggatc accagcccgg
     1081 ccagcgacat cgggtccatc gcgggaacct caggcacgcc cggtgcgatc accagcgaga
     1141 tcatgatgcc cagaccgacc ttcacgcgca ccggcacgct gacgttgccg aacagcggcg
     1201 cggttgcgat cagtccgagc acgcgcgtgg ccggccacag gaacgaggcg acccaggtat
     1261 agagctcggt gctgctgaaa gtgatcatgg ttcggccggc cagcggccct cgacgggcct
     1321 agccgatgaa tccgggaatg ccggtcagca tgtggcgcat gtagttggcc atcaccgaca
     1381 gcatccatgg cccggcgatc atcatcgcga ggaagatccc gatcagcttc gggatgaagg
     1441 acagcgtcat ctcgttgatc tgggtggcgg cctggaagat gctgaccacc agaccgacgg
     1501 ccagcgcggt cagcagcatc ggtgcggcga ccatcagggt gacttcgatg gcctggcggc
     1561 cgatggtcat gacgctttcc ggactcatgt cgggctcctc aatagaaact ttgcgccagc
     1621 gagccgatca gcagctgcca gccgtcgacc agcacgaaca gcatcagctt gaatggcagc
     1681 gatacgatcg ccggcgacat catcatcatg cccatcgaca tcaggatcga tgccacgacc
     1741 atgtcgatga tcaggaacgg gatgaagatc gcgaacgcga tctggaaggc ggtcttcagc
     1801 tcgctggtga tgaaggcggg aatcagcacg cgcagcggca cgtcctcggg accgttcagc
     1861 gcaggcccgc cggaaagccg cacgtacagc gcgaggtcgg attcgcgcgt ctggcgcagc
     1921 atgaaatcct tcagcggcac cgcgccgcgc tcgagcgcgt ccgacatcgt gatcttgttt
     1981 tccgccagcg gctggtaggc ctcggcgtag atgcggtcga acaccggccc catcacgaac
     2041 agcgtcagga acagcgccag gccgatcagc acctggttcg gcggcgcggt ctgcgtgccc
     2101 agcgcctggc gcagcagcga cagcacgatg atgatgcgcg tgaagctggt catcatcagc
     2161 agcgccgccg gcagcatggt cagcgccgtc aacagcagca gggtttgcag gctcagcgta
     2221 taggtctgcg cgccgcccgg ccccggggtg ctggtgaacg cgggcaggcc ggtctgcgcg
     2281 gatgcgaaaa gcggcaggcc caggagcgcc agcggcagca ggaagcggaa ggtcttgaat
     2341 cccatcgggg agatgctggt gatcagttga agtttgcggc cggcatggag cggccacgcg
     2401 caggtcagcc cgcgttgcgt ttctcgatgg ttttcctgag ccatgccgag aactcggaag
     2461 actgcgcggg agccggatga gtcggaatga tttcctggcg cggcagtgac gacagcgcgt
     2521 tcacgcggcc gggggcaacg ccgaccacga tccactggtc ggccacctcc accaccatca
     2581 cgcgttcgcg attgcccacg ctcacgccgc cgaccacgcg cgcgacattc gcggtgccgg
     2641 tgcccgccac gcccatgcgc ctgagcagcc aggccgcgcc cgccatcagt gcgagcacga
     2701 cgaccaggcc gaacagcacc tgcagcaggc ttccggcggt gccagcgggc gcatcggcgc
     2761 cgaatgcggg tgccgcgccg gcgagaagga acaggggaag ggcgtgcttc atcggcgcgg
     2821 cgtcagcggt tcagttttcg gatgcgctcg gacggcgtga tgatgtcggt cagtcggatg
     2881 ccgaacttgt cgttgaccac caccacctcg ccctgtgcga tcaggcagcc gttgacgaga
     2941 acgtccatcg gttcgccggc aagcccgtcc agttccacca ccgagccctg cgcgagctgc
     3001 agcaggttct tgatcgcgat cttcgtgcgc ccgagttcca cagtgagctg caccgggatg
     3061 tcgaggatga aatcgatgtc gttgtgggtg ccagttcgca ccgttccgga caggtcctgg
     3121 aacatgtggg cagcagtctg cttggactgc agcgcggcct gttcggcctg ctgctgctcg
     3181 gcaaacgcgg cgccccaatc gtcgtccgcg gccgtttggc taccgttttc gtcagccatg
     3241 atcatctcct tgcgtgaaat cgttggtgct ataggtcagc agcttttcca cgcgcagcgc
     3301 atattgaccg ttgaattttc cgtagctgca ttccatgacc ggaacgccat ccacttttgc
     3361 cgccaccttc tccggtatcg tgatcggaag cacatcaccg gccttcaggt tcagcacctc
     3421 gcccagcgtg gccttggcgg tgccgagatc ggccaccagc tcgacctcgg cggtctggat
     3481 ctgttgcgtc atcaggcgca cccaacgctt gtccgtctcc agcgtttcgc cctgcaggct
     3541 gctggtcagg atgtcgcgga tcggctcgac catcgcatat ggcgtgcaga agtgcatttc
     3601 gccgctgacc ggacccagtt cgatcgtgaa ggtcgtcgcc accacgactt cattcggggt
     3661 ggcgatattg gcgaactggg tgttcatctc cgaacggacg tactcgaatt ccaccggata
     3721 caccggttcc cacgacttgg cataggtttc gaagacgatg ccgagtacgc gctggatgat
     3781 gcgctgctcg gtctgcgtga agtcgcggcc ctccacgcgg gtatgaaagc gtccgtcccc
     3841 gccgaacagg ttgtccacca gcaggaacac caggttcgga tcgaacacga tgagcgcggt
     3901 gccgcgcagc ggcttcatgt gcaccaggtt caggttggtc ggcaccacca ggttgcggat
     3961 gaattcgctg tacttgtgga cgcgcaccgg gccgatcgag acctctgcgc tgcgatgcag
     4021 gaaattgaac aggccgatgc gcaacaggcg cgcaaagcgc tcgttgatga tttccagcgt
     4081 gggcatgcgc ccgcggacga tgcgttcctg cgttgcgagg ttgtacgggc gtatgcccga
     4141 ctcgtcctcg gcggcggcgt ggtcgtcctc ttcgccggtt acgcccttca gcagggcatc
     4201 gacttcttcc tgggaaagga aactgtcgga catggcatca ctggatcacg aacgacgtga
     4261 agaagacccc ggtcacgccg accggcgcgc cattgggtgc gaacggttcc tgcaggcgcg
     4321 caacgatttc ctcctgcagc ttttccttgc cctcggcggt gctcagctcg gacggcttct
     4381 tgctcgacag gagcatcagc agccggctgc gcaccagcgg catgtacagc ttgatcaggt
     4441 cggcctggga ttgatcgctc acctgcagcg tgaaggcggt ctgcaggtag tgctcgccca
     4501 tttcctgctg caggttcacg gtgaactggt cgatcggcag gtagagcggc ggcttcgggg
     4561 tttcggcttt ggccgccgct tccttttccg ccttggcgcc catgaagaac catgcgccgg
     4621 cgccgccggc acccatcagc agcaccgccg cgacgacgag cagcttgacg ggggatttct
     4681 tcggcggcgg cgccttcgcg ccgtcgtcga tctgaaccac cttcggacct gccttcgaag
     4741 ctgttgccat ctcgttacct ttgttcttga tgccggggcg gctgagtcat gcacagtgcc
     4801 gcgggtgcat tgctgtcttg ctgcattcgc tgttcatgtc gctcgcgcag ccgggatccg
     4861 gttccggcgt tcgcacatct gcacaggtcc gaaccgggct cccgtctgcg cgcgagcgac
     4921 cgggttggag ttgtgcctcg ttccgatcgc catctcgccg ttaccggcgc agtattggcg
     4981 gaattatcct gaattccctc tgttgcggat gcgacgaaaa gaaggatgaa gccgatgcaa
     5041 atcctcgctt gacgtcacgg ctacgcaaac gtgtcgacaa ggccgattcc tgacgaccgg
     5101 gcaggggaga cggtggcgcc ggcttgcggg gcggcttccg caccgggctc gtcgccgg
//
LOCUS       DATLGF010000206        13494 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_100176, whole genome shotgun sequence.
ACCESSION   DATLGF010000206 DATLGF010000000
VERSION     DATLGF010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 13494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13494
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100176"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VM406_06165"
     CDS             <1..844
                     /locus_tag="VM406_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVL75581.1"
                     /translation="LTDNLLKQIEPFGATFHLGEEVTTVERQDDGRFYVETSKGTRFL
                     TRTVFIAAGVGAFQPRALKVDGIEKFDNTQLFYRVKDPARFHGRNLVICGGGDSALDW
                     VLNLVGKAESVVLLHRREEFRAAPASVAKMKELCDSYEMQFLVGQVTGFEENEDKLSE
                     IKVTGADGVTRRLPLDDLLVFFGLSPKLGPIAEWGLDIERKQLKVDTEKFETNIPGIF
                     AVGDINTYPGKKKLILSGFHEAALAAFGAAPYIFPEKKIHMQYTTTSPKLHKILGVES
                     PVFD"
     gene            complement(912..1388)
                     /locus_tag="VM406_06170"
     CDS             complement(912..1388)
                     /locus_tag="VM406_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75582.1"
                     /translation="MRIANWMAGGMLAMAAWTAHAGELRRFDADSMQSLVAVQQGKPF
                     VLVLWSLDCAYCEQSLKTLADEQKRRKDLRVVTLSTDSLDDPDVQAAMQAKLRRTGVK
                     SVAWAFGSAPPEQLRYAVDPKWHGEMPRSYWFTGEGAPKAYSGLISREIIGRMRGK"
     gene            complement(1385..2602)
                     /locus_tag="VM406_06175"
     CDS             complement(1385..2602)
                     /locus_tag="VM406_06175"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HVL75583.1"
                     /translation="MTDIRRRIAAFALGVLTLPAFAHDGHAGKGAAQSRPPRPELASS
                     AAFDAQGRLWVAYKLNEAGRQFVAVQSSDDLGNTWSAAQRAQQEPEPVSADGENRPKI
                     AFGPGGDMYLSYTKPLSKPYTGDIRFVRSQDGGRSFTAPVTVHANRDLITHRFDSLIV
                     DREGRVYVTWIDKRDLHAAEGRKQAYRGAAVYYAVSTDRGASFNGDFKIADHSCECCR
                     IALALDEQGRVAAMWRHVFAPNARDHAWAVLGPEGRTGEVQRASFDDWRVDACPHHGP
                     GLAFGRDGTRHQVWFNVKDGEGGVFYASVDRNGVLGAPTRLGSGQVAHADVAVLERRV
                     VLAWRQFDGKASSVVARVSHDEGRTWQETEVARTAGASDQPRLVAGAGRIVLVWRTER
                     EGFVVRTLAGDGT"
     gene            complement(2611..3378)
                     /locus_tag="VM406_06180"
     CDS             complement(2611..3378)
                     /locus_tag="VM406_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVL75584.1"
                     /translation="MPPPAPRSGPVRATSAARAMRCRRWRRSKARRRTISSARGCALA
                     TRPAGNAPDLRAEVSDAKELSLLRETALGSLRISVFEDDVRDAIFLQRSMVGAATVNT
                     VQNVDRVRTRGVEAALQSNNLFVNGLDLQAGLTLAQARILANAVVPDSGGKGWPRVPR
                     LRAPALASYRSGPWTSSIGVRHEGRQYGTLDNSDVIGNKHGGVSSFTVVSARVAYDRA
                     RVGRLAFGVENLADRRYYVGPHPYPGRTGYAEAQFAF"
     gene            3275..3508
                     /locus_tag="VM406_06185"
     CDS             3275..3508
                     /locus_tag="VM406_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75585.1"
                     /translation="MVRLLALLRRHRRHRMARAADVARTGPDLGAGGGIAQQAAVVYP
                     IVVGPRRIEAVDLQRGRERIAGLEPGLVLHAAL"
     gene            complement(4390..4761)
                     /locus_tag="VM406_06190"
     CDS             complement(4390..4761)
                     /locus_tag="VM406_06190"
                     /inference="COORDINATES: protein motif:HMM:NF022610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 domain-containing protein"
                     /protein_id="HVL75586.1"
                     /translation="MNPVPPRIHSRLAWIALFVLLLQALAPVVVRALPGQMQTAEICT
                     ALGVQTVQIAAQDGEPGDESATNHCPWCKLSDTAPLLPPPDSGARPVAGAGPPAAVQV
                     PYRPLPTLRAAYPRGPPPSLA"
     gene            5198..6433
                     /gene="phaZ"
                     /locus_tag="VM406_06195"
     CDS             5198..6433
                     /gene="phaZ"
                     /locus_tag="VM406_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HVL75587.1"
                     /translation="MLYQIHELNRTFLNPLIQWAEASAKLFTNPVSPLAHTPFAQRIA
                     AGYELMYRLGKDYEKPEFGIKTVKVAGKDVGVLELVDMHKPFCRLLHFKKDLSDKAFA
                     DLKQPTVLVVAPLSGHHSTLLRDTVRALLSDHDVYITDWTDARMVPLSEGPFHLHDYI
                     YYVQEFIRKLGPDVHVISVCQPTVPVLAAISLMATEKDPKLPKTMTMMGGPIDPRKSP
                     TAVNDLATKKPYAWFEDKVIYSVPANYPGFGRKVYPGFLQHAGFIAMNPGRHAQSHWD
                     FYMHLREGDQASAEEHRKFYDEYNAVLDMPAEYYLETIKVVFQDFQLPLGTWEVEGKP
                     VRPQDIRNVALFTIEGELDDISGAGQTQAAHELCKNIPESKKQHFTAPKCGHYGIFSG
                     RRWREIICPKIGEFIRAHG"
     gene            6546..7328
                     /gene="rsxB"
                     /locus_tag="VM406_06200"
     CDS             6546..7328
                     /gene="rsxB"
                     /locus_tag="VM406_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HVL75588.1"
                     /translation="MSDSPRPLADQIEDLLPQTQCTKCGFPACRPYAEAIASGEANYN
                     QCPPGGAEGIARLAALLGKPVIPLNPANGVERPRAVAVIDEAICIGCTLCIQACPVDA
                     IVGAAKQMHTVVPQLCTGCDLCVAPCPVDCIAMVEVTPGRTGWDAWSQEQANDARARH
                     QFRTFRLRRDKEENDARLAAKAAAKLRAVEAQASLSPEEKAAQERKKAIIAAAMERAR
                     LKKEQMAAQAADAPTGIDDVSRGVRRQIEEVQARMDQQDDKP"
     gene            7325..7975
                     /gene="nth"
                     /locus_tag="VM406_06205"
     CDS             7325..7975
                     /gene="nth"
                     /locus_tag="VM406_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HVL75589.1"
                     /translation="MNNAKRLEIFTRFRNANPHPTTELEYTTPFELLIAVLLSAQATD
                     VSVNKATRRLYAVANTPAAILEMGEEKLADYIKTIGLYRTKAKNVIATCRILVEQHGG
                     EVPRSRDALEALPGVGRKTANVVLNTAFGEPTIAVDTHIFRVSNRTGIAPGKNVDEVE
                     RKLLKVVPPEFRHDAHHWLILHGRYICIARKPKCWNCVIADLCEYKDKTPLPEKGV"
     gene            8042..8467
                     /locus_tag="VM406_06210"
     CDS             8042..8467
                     /locus_tag="VM406_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HVL75590.1"
                     /translation="MFTPSQHDVRRFFCETFGKMRANEILTPMEALARDWIVQHPEYE
                     PALAHVEDALAADYSIERGESNPFLHLAMHLTIEEQLSIDQPAGIRAAFEALARRRGS
                     AHDAHHEMMECLGEMIWRSQRSGLPPDGAQYLECVRKRV"
     gene            complement(8684..9190)
                     /locus_tag="VM406_06215"
     CDS             complement(8684..9190)
                     /locus_tag="VM406_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HVL75591.1"
                     /translation="MKFEDLKQGFSSFWDSMSEGWQHLRQTASNALTGFRPSEQTNLP
                     ARSQVDDTFYMPSIGWSMLGGDVFEDEDRVVVRLEVPGMDKNDLDVEVEGGMLVVRGE
                     KRFQREESEGRYRVLQCAYGSFRRAVPLPVDVLSDQAKASYRDGVLRVELPKVRPEAP
                     KRQTVRVD"
     gene            complement(9219..9539)
                     /locus_tag="VM406_06220"
     CDS             complement(9219..9539)
                     /locus_tag="VM406_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HVL75592.1"
                     /translation="MPLVDVFEDGKGITLYADLPGVPKDKLHLQLESDSLTIEGEVSL
                     DTPGEMESSHAEVRLPRYRRVFSLSRELDTDKAQAELKNGVLKLRIPKAEHAQPRKIE
                     VKVT"
     gene            complement(9644..10081)
                     /locus_tag="VM406_06225"
     CDS             complement(9644..10081)
                     /locus_tag="VM406_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HVL75593.1"
                     /translation="MFRTMFPGDLFAELDRLQREVQQAFEGGPSIRGFGRGGYPMLNV
                     GNTPQSIELYAFAPGLDPQKIEVNLERGVLTISGEREADKPEDEKATVHIGERFAGRF
                     RRVISLPDEDINPSGVSAQYRDGVLHVSVKRRESAQPRRIEIQ"
     gene            10256..11200
                     /locus_tag="VM406_06230"
     CDS             10256..11200
                     /locus_tag="VM406_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HVL75594.1"
                     /translation="MEFKDYYQVLGVKRDATADEIRKAFRRLARKYHPDVSKEPEAET
                     RMKEVNEAFAVLSDPEKRAAYDQVGQGMRAGQEFRPPPNWDSGFEFSGAGFSQADANG
                     FSDFFSELFGHQMGGARRRAHGGFRMRGEDHHAKIVLDIEDAYHGPTRSVTLRAPATD
                     ALGHVMLSERTLNVDIPKGIREGQMIRLTGQGSPGAGGGPNGDLFLEVHFAPHPRYRA
                     EGRDVYARLPLAPWEAALGATVQATVPDGAVELRIPPGTQSGKKLRLKGRGLPGNPPG
                     DLYLVADIVLPPATDPAARALYERMARELRFDPRTGAA"
     gene            11261..11683
                     /gene="trxC"
                     /locus_tag="VM406_06235"
     CDS             11261..11683
                     /gene="trxC"
                     /locus_tag="VM406_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HVL75595.1"
                     /translation="MHIVCPHCHTTNRVPAERLAQHPDCGRCSRPLFSGQPASLDGAA
                     FQRHLERSDIPLLVDFWAPWCGPCRMMAPAFEEAAGQLEPQFRLAKVNTDAEQALAAR
                     YQIRSIPTLMVFRGGSEVARQPGAMGAGDIVRFARAAG"
     gene            11725..11913
                     /locus_tag="VM406_06240"
     CDS             11725..11913
                     /locus_tag="VM406_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75596.1"
                     /translation="MLASQMEDKCALPRCTCAVPVGQPYCSESCRREHADPDEQGTEC
                     PCGHAECTSAGPSGGVTL"
     gene            complement(11936..12991)
                     /locus_tag="VM406_06245"
     CDS             complement(11936..12991)
                     /locus_tag="VM406_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010089352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HVL75597.1"
                     /translation="MRQNLLAMILPRPPSLTLSSHFLVAALRGLGEQQGPAAARQALE
                     AAGIPPHLLEQPAARITREQFVLLYKAVAAALDDEMLGLWSRPIRGGTLKYLMLSLLN
                     APSLQIALNRFVRFWNLILDDYRLQMSRRQNEAVRLALVPRQQGVCVRPLGHELMMKL
                     VHGVASWLLGRQIAQVRVEFVFPRPGHASDYVFLYPGEVCFDAAETCIYLPDGDCQAA
                     FQRERHQLWTFLKRAPEDWVFTTLQRSSAAARTQACLERALDQALTVREVAAALNMSV
                     RTLNRKLASERTSFQALKDAVRRDVAVQRLSQSGAPIATVAADLGFANAAVFCRAFRQ
                     WTGSTPSAYRRGARRRA"
     gene            13139..>13494
                     /locus_tag="VM406_06250"
     CDS             13139..>13494
                     /locus_tag="VM406_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVL75598.1"
                     /translation="MTYAALEVDMDGPVAVVTLSRPERRNALDEQAVGELEHWFAQPP
                     KDVRAIVLAARGPHFCAGLDLKEHYLKARSPAEFMRVCQGWHRAFDRIQHGGIPVVAA
                     MQGAIVGGGLELAGAA"
BASE COUNT         2374 a         4534 c         4347 g         2239 t
ORIGIN      
        1 actcacggac aacctgctca agcagatcga acccttcggc gccaccttcc acctgggcga
       61 agaagtcacc accgtggagc gccaggacga cggccgcttc tacgtggaga ccagcaaggg
      121 cacgcgcttt ctcacgcgca ccgtcttcat cgccgccggc gtgggcgcct tccagccacg
      181 cgcattgaag gtcgacggca tcgagaaatt cgacaacacc cagctgttct accgtgtcaa
      241 ggatccggcc cgcttccacg gccgcaatct ggtgatctgc ggcggcggcg attcggcgct
      301 ggactgggtg ctgaacctcg tcggcaaggc cgaatcggtc gtgctcctgc accgtcgcga
      361 agagttccgc gcggcgccgg cgtcggttgc gaagatgaag gaactgtgcg acagctacga
      421 gatgcagttc ctggtcggac aggtgaccgg cttcgaggag aacgaagaca agctcagcga
      481 gatcaaggtg accggcgccg acggcgtgac gcgccgcctg ccgctggacg acctgctggt
      541 gttcttcggc ctgtcgccga agctcggccc gatcgccgag tggggcctgg acatcgagcg
      601 caagcagctg aaggtcgata ccgaaaagtt cgagaccaac atccccggca tcttcgcggt
      661 cggcgacatc aacacctatc ccggcaagaa gaagctgatc ctctccggtt tccatgaggc
      721 cgcactcgcc gccttcggcg ctgcgcccta catcttcccg gagaagaaga tccacatgca
      781 gtacacgacg acctcgccga agctgcacaa gatcctcggc gtggaatcgc cggtgttcga
      841 ctgagccggc gcgctcttgc atgaaacggg ccccttgcgg gcccgttttt cttgatggcg
      901 ggatccgtcc gctactttcc tctcatgcgc ccgatgatct cccgcgatat cagcccggaa
      961 taggctttcg gcgctccttc ccctgtgaac cagtaactgc gcggcatttc gccatgccac
     1021 ttcgggtcga ccgcataacg cagttgttcg ggcggcgcgc tgccgaaggc ccaggcgacg
     1081 cttttcacgc cagtgcgccg cagcttggcc tgcatcgcgg cctgcacgtc cggatcgtcg
     1141 aggctgtcgg tggacaaggt gacaacgcgc aggtccttgc ggcgcttctg ctcgtcggcc
     1201 agggtcttga ggctctgctc gcaatacgcg cagtcgagcg accacagcac cagcacgaac
     1261 ggcttgccct gctggaccgc caccaggctc tgcatgctgt cggcatcgaa gcgtcgcagc
     1321 tcacccgcgt gcgcggtcca cgcggccatc gcgagcatgc cgcccgccat ccagtttgcg
     1381 attctcatgt accgtctccc gccagtgttc tgaccacgaa tccctcccgt tccgtgcgcc
     1441 ataccagcac gatgcggccg gcgccggcaa ccagtcgtgg ctggtcggat gcgcccgcgg
     1501 tgcgcgcgac ctcggtctcc tgccaggttc gaccctcgtc gtgcgagacc cgggctacca
     1561 cgctgctggc cttgccgtcg aactgccgcc acgcgagcac cacgcgccgc tccagcaccg
     1621 ccacgtcggc gtgcgcgacc tggccgctcc ccagccgggt cggggcgccg agcacaccgt
     1681 tcctgtcgac actcgcatag aacacaccgc cttcgccgtc cttcacgttg aaccagacct
     1741 ggtggcgcgt gccgtctctg ccgaaggcca ggcccggtcc gtggtgcgga caggcgtcca
     1801 cgcgccagtc atcgaagctt gcgcgctgca cctcgcccgt ccgcccttcc gggccgagga
     1861 ccgcccacgc gtggtcgcgc gcattcggcg cgaacacatg gcgccacatc gcggcgacac
     1921 ggccctgctc atcgagcgcg agcgcgatgc ggcaacattc gcagctgtgg tcggcaatct
     1981 tgaaatcgcc gttgaaactg gctccgcgat cggtcgacac cgcatagtag acagcggcgc
     2041 cgcgatacgc ctgcttgcgg ccctcggcgg catggaggtc gcgcttgtcg atccaggtga
     2101 cgtacacgcg gccctcgcgg tcgacgatca gcgaatcgaa gcgatgcgtg atcaggtcgc
     2161 ggttcgcatg cacggtgacg ggcgccgtga agctgcgccc gccgtcctgc gaacgaacga
     2221 agcggatgtc cccggtgtag ggcttggaca gcggcttggt gtagctcaga tacatgtcgc
     2281 cgcccgggcc gaaggcgatc ttgggacggt tctcgccatc ggcggacacc ggctccggct
     2341 cctgctgcgc gcgctgcgcg gcgctccagg tgttgccgag gtcgtccgag gactggaccg
     2401 ccacgaattg gcggcccgcc tcgttgagct tgtatgcgac ccacagcctt ccctgcgcat
     2461 cgaaggcggc ggaactggcc agttccgggc gcggcggccg ggattgagcg gcccccttcc
     2521 cggcgtggcc atcatgggcg aatgccggca gcgtgagcac gcccagcgcg aacgcggcga
     2581 tgcggcgtcg gatatctgtc atcgttgacc ctagaaagcg aactgggctt cggcgtaccc
     2641 ggtacgcccc ggatacgggt gcggaccgac gtagtagcgc cggtcggcca gattctcgac
     2701 gccgaacgca aggcggccga cgcgcgcccg gtcgtaagcg acgcgggcgc tcacgacggt
     2761 gaacgaggaa acgccgccat gcttgttgcc gatcacgtcg ctgttgtcca gcgtgccgta
     2821 ctggcggcct tcgtgccgca cgccgatgga acttgtccac ggcccgctgc ggtagctggc
     2881 gagcgccggc gcacgcagcc ttggcacgcg cggccagccc ttcccgccgg aatccggcac
     2941 caccgcattg gcgaggatgc gcgcttgcgc cagcgtcagg ccggcctgca ggtccagacc
     3001 gttgacgaac aggttgttgc tctgcaatgc cgcctcgacg ccgcgcgtgc gtacccggtc
     3061 gacgttctgc accgtgttga ccgtggccgc gccgaccatc gagcgctgca ggaagatcgc
     3121 atcgcgcacg tcgtcctcga acacggagat gcgcaggctg ccgagggcag tttcgcgcag
     3181 cagggacaac tccttcgcgt cgctgacttc ggcgcgcagg tcgggcgcgt ttccagccgg
     3241 tcttgtggcg agtgcgcagc cgcgcgccga actgatggtg cgcctcctcg ccttgctgcg
     3301 gcgccatcgt cggcaccgca tggcgcgtgc cgccgatgtc gcccgcaccg gaccagatct
     3361 cggcgccggt ggcggcatcg cgcagcaggc tgcggttgta tacccgatag tcgttggtcc
     3421 aaggcgcata gaagccgtcg acctgcagcg tgggcgagaa cgcatagccg gccttgagcc
     3481 tggcctggtg ctgcacgccg cgctgtaggc cgcttgcccc gacgatcatg cggtccgcgc
     3541 ccgtgggatc gaggtcggcg acggcgccgg tggcgacggg cgcggcgacc ccggcctgcc
     3601 gcgcggccga cgcgtagccc atcgcctggg aagcgtactc cagccggtac gccgccaatc
     3661 ccgcgctcag cgcaacccag cggttggcaa ggcagaagga ttgctgcttg ctgccatggt
     3721 gctcgcgcaa gccgtagtcg tcgtgcgact gccggtgcag ctcaattttc gccgctccct
     3781 cgaaagcccg cggctggcgg gtagtgattg agaccgtggt gccgatcgag ttgctcggat
     3841 agagcgccga gaacggcccg tagagcacgt ccacgtgctc gatctcctgc ggcgccacga
     3901 tgctccagcg cggcgcgtcg aaacggccga ggaagttgga gatcagatag ccatcgacat
     3961 aggccagtcc gcgtgccggc tgggtggtgc cgaacgagcg tccgccgagg ttcgcgttgc
     4021 ggtcgccgat gaagcgcttg cgcaccgtcg tgctgggtgc gtacaggagt gcatcgtcgg
     4081 tgctggtggt cggttgaaca gcgagcgcct cgcgcgtgac cgagaacgtc gacgccggca
     4141 ggttgggatc gagcggcgaa cgcgtcgcgc tcacggcgat cggcgccagc atggtttcgg
     4201 tggccacccc ttgggcggcg gcatccgggc aacggcaagc gcgcccgtca atacgggagc
     4261 gagagccagc gcagcccccg cccgaaagtg agaatgttgt gccatgaata cagcgacgcc
     4321 tcgggcgtcg gatagtcgaa cgcctgccgc ggtggcggca ggaccggcgc cctcagggcg
     4381 cggaatggtt caggcgaggg agggaggcgg accgcgcggg taggcggcac gcagcgtggg
     4441 aaggggacgg tacggaacct gaaccgccgc gggagggccc gcgccggcaa ccggtcgcgc
     4501 gccgctgtcc gggggcggca acagcggggc cgtgtcggac agcttgcacc atgggcaatg
     4561 gttggtcgcg ctctcgtcgc ccggttcgcc gtcctgcgcg gcgatctgga cggtctgcac
     4621 tccgagcgca gtacagatct ccgccgtctg catctggccc ggcagcgcac gcacgacgac
     4681 cggcgccagc gcctgcagca acaggacgaa caaggcgatc caggcgagtc ggctatgtat
     4741 gcggggcggg accggattca cgggtaaaac ttacaaacga gagggctggc gcaccggagg
     4801 aagctgtcca aaggcgcaag attatcgccg atttcagtgt cgattccgcg gctcatttcc
     4861 ggccggtagt gggcacggtt cctgcattgt ttcctcgcat cgaacatcct cctttgtgcg
     4921 gcctagcact tttctgccga cggaccatcc tcatattgag cctactcatg ttggaaacgt
     4981 cggaatggcg taacaccaag tgcagcaagg ccaagaatgc aaaggtgtgg tttgtgttat
     5041 gttttgccgc agcgaaactc gtcaagtaaa cgcggcgaag tgtcactgaa tatttcaggt
     5101 ttcaaataaa cctgagcttt tgtcagtgat gcattgcaac aagaaacgtt tatctgcaga
     5161 atttcgcgat accggacaaa cccgaaagag ggcattcatg ctttaccaaa tccacgagct
     5221 gaaccgcacg ttcctcaacc cgcttatcca gtgggccgag gcgtcggcaa agctgttcac
     5281 caatccggtc tcgcccctgg ctcatacacc gttcgcgcag cgcattgccg ccggttacga
     5341 actcatgtac cgcctcggca aggattatga aaaaccggaa ttcggcatca agacggtcaa
     5401 ggtcgccggc aaggacgtcg gcgtgctcga gctcgtcgac atgcacaagc ctttctgtcg
     5461 cctgcttcac ttcaagaagg atctgagcga caaggcattc gccgatctca agcagcccac
     5521 cgtcctggtc gtggcgccgc tgtcgggcca ccattcgacg ctgctgcgcg acaccgtgcg
     5581 cgcgctgctg tccgaccatg acgtctacat caccgactgg accgacgcgc gcatggtgcc
     5641 gctgtcggaa ggaccgttcc acctgcacga ctacatctac tacgtgcagg aattcatccg
     5701 caagctgggc ccggacgttc acgtgatctc ggtctgccag ccgaccgtgc cggtgctggc
     5761 cgcgatctcg ctgatggcca ccgagaagga cccgaaactg ccgaagacga tgacgatgat
     5821 gggtgggccg atcgacccgc gcaagtcacc caccgccgtc aacgatttgg cgacgaagaa
     5881 gccgtatgcg tggttcgagg acaaggtgat ctacagcgtc ccggcgaact accccggctt
     5941 cggccgcaag gtttatccag gcttcctgca gcatgccggg ttcatcgcga tgaatccggg
     6001 ccgccatgcg cagagccact gggacttcta catgcacctg cgcgaaggcg accaggcgtc
     6061 cgccgaagag caccgcaagt tctacgacga gtacaacgcg gtgctggaca tgccggcgga
     6121 atactacctg gagacgatca aggtcgtgtt ccaggacttc cagctgccgc tcggcacctg
     6181 ggaagtggaa ggcaagccgg tgcgtccgca ggacatcagg aatgtcgcgc tcttcaccat
     6241 tgaaggcgag ctcgacgaca tctccggcgc cggccagacc caggccgcgc acgagctgtg
     6301 caagaacatc ccggaatcga agaagcagca tttcacggct ccgaagtgcg gccactacgg
     6361 cattttctcc ggccgtcgct ggcgcgagat catctgcccg aaaatcggcg agttcatccg
     6421 cgcgcatggc tgattgttga tccagccgct cgtaccccga gaagccgcag tcgaccgact
     6481 gcggcttttt gctttaccgg cctccgatgg ccgataattc cgcttttccc agcccggttt
     6541 tccccgtgtc cgactcaccc cgccccctcg ccgaccagat cgaggacctg ctgccgcaga
     6601 cgcaatgcac gaagtgcggc tttccggcct gccgcccgta tgcggaagcg atcgcaagcg
     6661 gcgaagcgaa ctacaaccag tgcccgcccg gcggtgccga aggcatcgcg cgcctggccg
     6721 cgctgctcgg caagccggtg ataccgctca atcccgccaa cggcgtcgag cggccgcgtg
     6781 cggttgccgt gatcgacgag gcgatctgca tcggctgcac gctgtgcatc caggcctgtc
     6841 cggtcgacgc catcgtcggc gcggccaagc agatgcacac cgtcgtgccg cagctgtgca
     6901 ccggctgcga tctgtgcgtc gcgccctgcc cggtcgactg cattgcgatg gtagaggtca
     6961 cgccgggccg caccggctgg gacgcctggt cgcaggaaca ggcaaatgac gcgcgcgcgc
     7021 gccaccagtt ccgcaccttc cgcctgcgcc gcgacaagga agagaacgac gcgcgcctgg
     7081 ccgccaaggc ggccgcgaag ctcagggcgg tcgaagcgca ggcaagcctg agtccggagg
     7141 agaaggcggc gcaggagcgc aagaaggcga tcatcgccgc cgcgatggag cgtgcgcggc
     7201 tgaagaagga gcagatggcc gcgcaggccg ctgacgcgcc caccggcatc gacgacgtct
     7261 cgcgcggcgt gcgcaggcag atcgaggagg tccaggcgcg catggaccag caggacgaca
     7321 agccatgaac aatgcaaaac ggctggaaat cttcacccgc ttccgcaatg caaatcccca
     7381 cccgaccacc gagctcgaat acacgacgcc gttcgagctg ctgatcgcgg tgctgctgtc
     7441 ggcgcaggcg accgacgtat cggtgaacaa ggccacgcgc cggctgtatg cggtcgcgaa
     7501 cacgccggcg gcgatccttg agatgggcga ggagaaactg gcggattaca tcaagacgat
     7561 cggcctgtac cgcaccaagg caaagaacgt gatcgccacc tgccgcatcc tggtcgagca
     7621 gcacggcggc gaagtgccac gttcgcgcga tgcgctcgag gcgctgccgg gcgtcgggcg
     7681 caagaccgcc aacgtcgtgc tcaacaccgc gttcggcgag ccgacgatcg cggtcgacac
     7741 ccacatcttt cgcgtgtcca accgcaccgg gattgcgccc ggcaagaatg tggacgaggt
     7801 cgagcgcaag ctgctgaaag tggtaccgcc ggaattccgc cacgatgccc accattggct
     7861 gatcctgcac ggccgctaca tctgcatcgc gcgcaagccc aagtgctgga actgcgtgat
     7921 cgccgacctg tgcgagtaca aggacaagac gccgttgccg gaaaaaggag tgtgaggatt
     7981 cgaaacggac ggcggcggga gagcgcgtaa aatacccttc cctctgccgc gttcaacccc
     8041 gatgttcacc ccctcccagc acgacgtacg gcgcttcttc tgcgaaacct tcggcaagat
     8101 gcgcgccaat gaaatcctca cgccgatgga ggcgctcgcc cgcgactgga tcgtccagca
     8161 cccggaatac gagcctgcac ttgcgcacgt cgaggatgcg cttgccgccg actattcgat
     8221 cgagcgcggc gagagcaatc ccttcctgca tctggcaatg cacctgacga tcgaggagca
     8281 gctgtcgatc gaccagccgg ccggcatccg tgctgcgttc gaagccctcg cccgccgccg
     8341 cggctcggcg cacgacgctc accacgagat gatggagtgt ctcggagaaa tgatctggcg
     8401 ctcgcagcgc agcgggctgc cgccggatgg ggcgcagtat ctggaatgcg tgaggaagcg
     8461 ggtctgagat gaacttgcag gggcactgcc ggaataccgt tcatcaacgt gattgccctc
     8521 accatgtcgc ccccgcacag gcgggggccc agttccggcg tcagcacggc tgcctacgat
     8581 cgaactgggc ccccgcctgc gcgggggcga cagagcaagg gtagtcgctc ggggaaacgg
     8641 cattcgaccc cccgagcccc cgcttcgcgc gaggccgcgc ccttcaatcc acccgcaccg
     8701 tctgccgctt cggcgcctcg ggcctgactt tgggcaattc gacgcgcagc acgccgtcac
     8761 ggtagctggc cttggcctga tcggacagga cgtcgaccgg cagcggcacc gcgcggcgga
     8821 agctgccgta tgcgcattgc agcacgcgat agcggccttc cgattcttcg cgctggaaac
     8881 gcttctcgcc gcgcaccacc agcatgccgc cttcgacctc gacgtctaga tcgttcttgt
     8941 ccatgcccgg cacttcgagt cggacgacga cgcggtcctc gtcctcgaac acatcgccgc
     9001 ccagcatcga ccacccgatc gacggcatgt agaacgtgtc gtcgacctgc gagcgggccg
     9061 gcagattggt ctgttcgctc gggcggaagc cggtcagtgc gttcgacgcc gtctggcgca
     9121 gatgctgcca gccctcggac atggagtccc agaacgagct gaagccttgc ttgagatcct
     9181 cgaacttcat ggccaagctc ctttccgttc gacaggtctt acgtgacctt gacttcgatc
     9241 ttgcgcggct gcgcgtgctc ggccttcgga atgcgcagct tcagcacgcc gttcttcagt
     9301 tccgcctgcg ccttgtcggt gtcgagttcg cgcgacaacg aaaatacccg gcgatagcgc
     9361 ggcagccgca cttccgcgtg gctcgattcc atttcgccgg gcgtatcgag cgacacctcg
     9421 ccttcgatcg tcaggctgtc ggattccagc tgcaggtgca acttgtcctt cggcacgcca
     9481 ggcaggtcgg catacagggt gatgcccttg ccgtcctcga atacatccac cagcggcatc
     9541 agggccggtt ccggacgctg cacctcacgc ccgacatcgc gccgggtttc accgctcgcc
     9601 tgcggcagat tcctgttttc gctcatgatc tggtcctcct tgcttactgg atctcgatgc
     9661 gtcgcggctg ggccgattca cgccgcttga cactcacatg cagcacgccg tcgcgatact
     9721 gcgccgatac gccgctcggg ttgatgtcct cgtcgggcag gctgatcacc ctgcggaagc
     9781 ggccggcgaa gcgctcgccg atgtgcacgg tcgccttttc gtcttccggc ttgtctgctt
     9841 cgcgctcgcc ggaaatcgtc agcacgccgc gctccaggtt cacctcgatc ttctgcggat
     9901 ccagcccggg cgcgaacgcg tacagctcga tcgactgcgg cgtattgccc acgttcagca
     9961 tcggatagcc gccgcgcccg aagccgcgaa tgctcggtcc tccttcaaac gcctgctgaa
    10021 cttcgcgttg caggcggtcc agttcggcga acagatcgcc tgggaacatc gtacgaaaca
    10081 tcatgctgtc ctccgttgtg gttgaacacg cctcccctcg gcaacgcatg ccttgggcgg
    10141 cttgactttc tgcctccaaa ataggagcac aaaaaataat ttcaatacct tgatctgcgg
    10201 gctttccgcc ccgattctgg tgcggtagaa cgaacaaaaa tgatgggggc aacgcgtgga
    10261 attcaaggac tactaccagg tactgggcgt gaagcgcgat gccacggcgg acgagatcag
    10321 gaaagccttc cgccggcttg cgcgcaagta ccatccggac gtctcgaagg agccggaggc
    10381 cgagacgcgc atgaaggaag tcaacgaagc cttcgccgtg ctgtccgatc cggagaagcg
    10441 cgcggcctac gaccaggtcg ggcagggcat gcgcgccggg caggagttcc gcccgccgcc
    10501 gaactgggac agcggcttcg agttttccgg cgccggattt tcgcaggccg atgcgaacgg
    10561 attcagcgac tttttctcgg aactgttcgg gcaccagatg ggcggggcgc ggcggcgcgc
    10621 ccatggcggc ttccgcatgc gcggcgagga tcatcacgcg aagatcgtgc tcgatatcga
    10681 ggacgcgtat cacggcccca cgcgcagcgt gacgctgcgc gctccggcga ccgatgccct
    10741 cggccatgtg atgctcagcg aacgcacgct caacgtggac atccccaagg gaatacgcga
    10801 ggggcagatg atacggctca ccggccaggg cagccccggc gccggcggcg gaccgaatgg
    10861 agatctgttt ctggaggttc acttcgcgcc gcatccgcgc tatcgggcag agggacgcga
    10921 cgtctatgcg cggctgccgc tggcgccctg ggaggcggcg ctcggcgcca ccgtgcaggc
    10981 gaccgttccg gacggcgccg tggagttgcg catcccgccg ggcacgcagt caggaaaaaa
    11041 acttcggctg aagggacgcg ggctgccggg caacccgccc ggcgacctct acctggtcgc
    11101 cgacatcgtg ctgcctccgg caacggaccc cgcggcgagg gcgctatatg aacgcatggc
    11161 gcgggagctg cgcttcgatc cgcgcaccgg cgccgcctga ggcacggcgg acattcgctc
    11221 ggcgaagatc tcgcgccaag tcaacgcaag gaggaccgac atgcatatcg tttgcccgca
    11281 ctgccatacg accaaccgtg taccggccga gcggctggca cagcaccccg actgcggccg
    11341 ctgttcgcgc ccgctgtttt ccggccagcc cgcgagcctc gacggcgccg cgttccagcg
    11401 tcacctcgag cgcagcgaca ttccgctcct cgtcgacttc tgggcgccgt ggtgcggccc
    11461 ctgccgcatg atggcgcccg cgttcgaaga ggccgccggg cagctcgaac cgcagttccg
    11521 gctggcgaaa gtgaacacag acgcggaaca agccctggct gccaggtacc aaatccgtag
    11581 catccctacc ttgatggtgt ttcgcggcgg cagcgaagtc gcccgccagc ccggcgcgat
    11641 gggcgccggc gatatcgttc gcttcgcacg cgcggcgggc taagcctgcc ccgcatggcg
    11701 agcccttaag acggaggaag cttcatgttg gcgagccaga tggaagacaa gtgcgcactg
    11761 ccccgctgca cctgcgcagt gccggtcggg cagccgtatt gcagcgagtc atgccgccgt
    11821 gagcatgcgg acccggacga acagggaacc gagtgcccgt gcggacatgc cgagtgcacg
    11881 tcggccggac cgagcggtgg cgtgacgctg tgagcgcgct gccgtttgcc tgccctcagg
    11941 ccctcctgcg cgcaccgcgc cggtatgcgc tcggggtgct gccggtccac tggcgaaacg
    12001 cgcggcagaa caccgccgca ttcgcgaacc cgaggtcggc cgccacggtg gcgatcggcg
    12061 cgcccgactg gctcaggcgc tgcactgcca catccctgcg caccgcatcc ttgagcgcct
    12121 gaaagctggt gcgctcgctc gccagcttgc gattcagcgt gcgcaccgac atgttcagcg
    12181 ccgccgcaac ttcccgcact gtcagcgcct gatccagcgc gcgctccagg caggcctgcg
    12241 tgcgcgcggc ggccgagctg cgctgcagcg tcgtgaacac ccagtcttcc ggcgcgcgtt
    12301 tcagaaaggt ccagagctgg tgacgctcgc gctggaaagc ggcctggcaa tccccgtcag
    12361 gcaggtagat gcaggtctcc gccgcgtcga aacacacttc gccgggatac aggaatacat
    12421 agtccgacgc atgtcccggg cgcgggaaca cgaactcgac gcgcacctgt gcgatttgcc
    12481 ttcccagcag ccaggacgcc acgccatgga cgagcttcat catcagctca tggccgagcg
    12541 gcctgacgca cacgccctgc tgccgcggga ccagcgccag ccgcaccgcc tcgttctggc
    12601 ggcgcgacat ctgcagcctg tagtcgtcca ggatcaggtt ccagaaccgc acgaagcgat
    12661 tgagcgcaat ctggaggctg ggcgcgttca aaagactcag catcaggtac ttcagcgtgc
    12721 cgccgcgaat cggccgggac catagcccca gcatctcgtc gtcgagcgcg gccgccacgg
    12781 ccttgtacag caaaacgaac tgctctcggg tgatgcgggc agcgggctgt tcgagcaggt
    12841 gcggcggaat gccggcggcc tccagagcct gcctcgctgc agccggtcct tgctgctcgc
    12901 ccaggccgcg cagtgccgcg acaaggaagt gcgaggaaag cgtaagcgag ggcggacggg
    12961 gcaggatcat ggcaaggaga ttctggcgca ttttgcatat cgtgtggcac gatacgtcaa
    13021 cagttcggcg gccttctccc taaacttcca cgcatatccg cgaaattccc gcatcggcgc
    13081 gcgaccgtgt cacgcaggcc tatggcgcgc tttgtttatt cattgactgg agagagacat
    13141 gacctacgct gcacttgaag tcgacatgga cggcccggtc gcggtcgtca ccctgtcccg
    13201 ccccgaacga cgcaatgcgc tcgacgaaca ggcggtcggc gaactggaac actggtttgc
    13261 gcagccgccg aaggacgtgc gcgcaatcgt actggccgcg cgcgggccgc atttctgtgc
    13321 cggcctcgac ctgaaggagc actacctgaa agcgcgcagt ccggccgaat tcatgcgcgt
    13381 gtgccaggga tggcatcggg ccttcgaccg catccagcac ggcggcatac cggtggtggc
    13441 ggcgatgcag ggcgccatcg tcggcggcgg actggagctg gccggcgccg cgca
//
LOCUS       DATLGF010000207         5251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_115703, whole genome shotgun sequence.
ACCESSION   DATLGF010000207 DATLGF010000000
VERSION     DATLGF010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115703"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VM406_06255"
     CDS             complement(<1..799)
                     /locus_tag="VM406_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL75599.1"
                     /translation="MATRKSDYSEASIRVLKGLEPVKQRPGMYTRTENPLHIIQEVID
                     NASDEALGGFGKNIVVTLNADGSVSVEDDGRGIPVGLHPEEKVSTVEIVFTRLHAGGK
                     FDKGSGGAYSFSGGLHGVGVSVTNALSSRLDITVWRDGGVHQIGFADGDVVQKLKTRP
                     STKEDKKSGTRVTVWPNPKYFDSSAIPGAELQRLLRSKAVLLPGVKVTLINAKTGDTQ
                     VWQYDQGLRGYLVESLAQASHAEPLIPLFEGEQYANADMEGFAEGEGA"
     gene            1016..1789
                     /locus_tag="VM406_06260"
     CDS             1016..1789
                     /locus_tag="VM406_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL75600.1"
                     /translation="MAAAEQAFASSEDKGYGYFVLADDNLQDPDIYIVKGGDLRALAQ
                     LSELRPSDLRPALLIGKPAIDLPFAFVAAPATHADVVAALDRLVEKRADALVRLEASD
                     IVLVPERRRRGRLSLDLTDPAAYEKMRVRRPADGMVVVLDKSPALRDYLRDLTVRQQL
                     RVEWAGDEDGAIDLCRSAPTAVLLINTSTPEVNPYRVSLAVKDREAPFSTAVVFLVSR
                     PFVYDAEQARRVGVEGFLNKPLASQHLISLLKKYVPKLY"
     gene            complement(2010..3179)
                     /locus_tag="VM406_06265"
     CDS             complement(2010..3179)
                     /locus_tag="VM406_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HVL75601.1"
                     /translation="MLIDFFFTLKDAKVPVSIKEFLVLLEAMQKDVINPSLDEFYYLS
                     RTTLVKDEANFDKFDKAFGLYFRGIDTIFEKMPEIPLEWLQKRMERELTPEQRAAIEK
                     FGYDKLMERLKQLLDEQKERHEGGNRWIGTGGTSPFGNGGENPQGVRIGGEGGQRSAV
                     KVWEARSYRDYDSERELGTRNIKVALRRLRKFAREGAAEELALDDTIRATANNAGYLD
                     IKMQPERKNNIKVLMLLDVGGTMDDHIARTEELFSAAKTEFKNMEFYYFHNCVYDYLW
                     KNNRRRHAERFPTWDILRKYPPDTKLIFVGDATMSPYEVLQPGGSVEYNNEEAGAEWL
                     QRFTATFPKFIWLNPEPESLWQYRQSIAIIRQIMNNRMFPVTIDGLERAMHALSK"
     gene            complement(3169..3789)
                     /locus_tag="VM406_06270"
     CDS             complement(3169..3789)
                     /locus_tag="VM406_06270"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HVL75602.1"
                     /translation="MISLEPVTLEFNGIRLEPMTRAHAVNLAVAASDGALWNIRVTSV
                     PKPDETAAYVNNALSMLDAGTRLPFVVRELSGGKIIGSTSYHDILREVDRVEIGYTWY
                     AQSWQRTHVNTSCKLMLLRHAFETLGCKVVGFRTDNFNLASQRAIERLGARRDGVIRH
                     HALRRDGTVRDTVMYSITAGEWPEIEAHLKDKLAQRVLRDGGRRAD"
     gene            complement(3800..4654)
                     /locus_tag="VM406_06275"
     CDS             complement(3800..4654)
                     /locus_tag="VM406_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HVL75603.1"
                     /translation="MQPTTRFEGSAQYVATQDLKLAVNAALTLQRPLLIKGEPGTGKT
                     MLAEEVAGALGMPLMQWHIKSTTKAQQGLYEYDAVSRLRDSQLGDDRVKDIRNYIVKG
                     VLWQAFTADQQVVLLIDEIDKADIEFPNDLLRELDRMEFHVYETRETVRAKHRPLVII
                     TSNNEKELPDAFLRRCFFHYIKFPDRDTMAQIVDVHYPNLKKDLLAQALQTFYEVREV
                     AGLKKKPSTSELLDWLKLLLAEDIPPEALRSKDAKAVVPPLHGALLKNEQDVHLFERL
                     VFMARANR"
     gene            4900..5226
                     /locus_tag="VM406_06280"
     CDS             4900..5226
                     /locus_tag="VM406_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVL75604.1"
                     /translation="MKKLFAVLALAGAATTAFAQQTDIVGNPKAAENKIAMCIGCHGI
                     PGYRASFPEVYHVPMIGGQTQRYIEAALQAYKRGDRRNPSMRGIAAGLSDQDIADLAA
                     YYAQQK"
BASE COUNT          882 a         1632 c         1671 g         1066 t
ORIGIN      
        1 cggcgccttc gccctcggca aagccttcca tgtcggcgtt cgcgtactgc tcgccttcga
       61 acagcggaat cagcggctcc gcgtgcgagg cctgggccag cgactcgacc aggtaaccgc
      121 gcaggccctg gtcgtactgc cagacctggg tgtcgccggt cttcgcattg atcagcgtga
      181 ccttcacgcc gggcagcagc accgccttcg agcgcagcag gcgctgcaac tcggcgccgg
      241 gaatggcgga ggagtcgaag tacttcggat tcggccatac cgtcacgcgg gtgccggact
      301 tcttgtcttc cttcgtggac gggcgcgtct tgagcttttg cacgacatcg ccgtcggcaa
      361 agccgatctg gtgcacgccg ccgtcgcgcc agacggtgat gtcgaggcgg gaggagaggg
      421 cgttggtgac cgagacgccg acgccatgca ggccgccgga gaacgaatag gcgccgccgc
      481 tgcccttgtc gaacttgccg cctgcatgca ggcgcgtgaa cacgatctcg accgtcgaga
      541 ctttttcttc cggatgcaag ccgacaggaa tgccgcggcc atcgtcttct acgctgacgc
      601 tgccgtccgc attcaaggtg acaacaatgt tcttaccgaa gccgcccaag gcctcgtcgg
      661 aggcgttgtc gatcacttcc tggatgatgt gcagcgggtt ttcggtccgg gtgtacatgc
      721 ccgggcgctg cttgaccggc tcgagtccct tcaggacccg gatcgaggct tcgctgtagt
      781 cggattttct ggttgccatt cgcggtgtgt cggagcaagg ctgagtcaaa tcaagtcctg
      841 cattctaatc gagtcacttc agcggcgcgg ggtcggcggc ccgcagacgg catgccgaac
      901 atggaatgtg caaaaaaaga ctgttgaacg ccggaaaatg tttataaaat gcaatgctat
      961 gtcaacggcc gtatttcctt tcgcagtccg ccttttcggc ttcagccctc aggaagtggc
     1021 tgcggccgaa caggcattcg caagttccga ggacaagggt tacggctact tcgtgctggc
     1081 cgacgacaac ctgcaggacc ccgacatcta tatcgtcaag ggcggcgacc tgcgcgcact
     1141 tgcgcaactg agcgagctgc gcccgagcga tctgcgtccg gcgctgctga tcggcaaacc
     1201 ggccatcgac cttcccttcg ccttcgtcgc ggcgcctgcc acccatgccg acgtggtcgc
     1261 ggcgctcgac cgcctggtgg agaagcgcgc cgatgcgctg gtgcggctgg aagcctcgga
     1321 catcgtgctc gttcccgagc ggcgccggcg cggccgcctc agcctcgatc tgaccgatcc
     1381 ggccgcgtac gagaagatgc gcgtgcggcg tcccgccgac gggatggtcg tcgtcctcga
     1441 caaaagtccg gccctgcgcg attacctgcg cgacctgacg gtccggcaac agctgcgggt
     1501 ggaatgggcg ggcgacgagg acggtgcgat cgacctgtgc cgcagcgcgc cgacggccgt
     1561 tctgctgatc aatacgtcca cgccggaggt caacccgtac cgggtcagtc tggcggtgaa
     1621 ggacagggag gcgccgtttt ccactgcggt ggtattcctg gtgagcaggc cgttcgtcta
     1681 tgacgccgaa caggcgcgcc gtgttggcgt cgagggcttc ctgaacaagc cactggccag
     1741 ccagcacctg atctcgctgc tgaagaagta cgtgccgaag ctctactagg cggaggaggg
     1801 gcgccggcgc tcctgggcag gcgtaggggc ggggtgtcgt tctccgcttg ctttgcgttc
     1861 tcgcttgcca agcggatgag gtccctcact gcgttcgaga cgacaacata tgccggcgat
     1921 cgctcccaat cctgtcgttc cgagcgcagc gaggaaacgc atccggttcc attgccgctg
     1981 cgtcagaaat ttttcccccg cccgccgaac tacttgctca gcgcgtgcat cgcccgctcg
     2041 agcccgtcta tcgtcaccgg gaacatgcgg ttgttcatga tctggcggat gatcgcgatc
     2101 gactgccggt actgccacag gctttcaggc tcgggattga gccagatgaa cttcgggaag
     2161 gtcgcggtga agcgttgcag ccactcggcg ccggcttcct cgttgttgta ctcgaccgat
     2221 ccgcccggct gcagcacctc gtacggactc atcgtcgcgt cgccgacgaa gatcagcttg
     2281 gtatcgggcg ggtacttgcg caggatgtcc caggtcggga aacgctccgc gtggcggcgc
     2341 cggttgttct tccacaggta gtcgtagacg cagttgtgga agtagtagaa ctccatgttc
     2401 ttgaactcgg tcttcgcggc cgagaacagt tcttcggttc gggcgatgtg gtcgtccatc
     2461 gtgccgccga cgtcgagcag catcagcacc ttgatgttgt tcttgcgctc tggctgcatc
     2521 ttgatgtcga ggtagccggc gttgttcgcc gtcgcgcgga tcgtgtcgtc cagcgccagc
     2581 tcttcggccg cgccctcgcg cgcgaacttg cgcaggcgtc gcagcgcgac cttgatattg
     2641 cgggtgccga gttcgcgttc cgaatcgtag tcgcggtagc tgcgcgcctc ccataccttc
     2701 accgcgctgc gctggccgcc ttcgccgccg atgcgtacgc cctgtggatt ctcgccgcca
     2761 ttgccgaacg gcgaggtgcc gccggtgccg atccagcggt tgccgccctc gtggcgctcc
     2821 ttctgttcat cgagcagctg ctttaagcgc tccatcagct tgtcgtagcc gaacttctcg
     2881 atcgccgcac gctgctccgg ggtcagctcg cgttccatcc gcttctgcag ccattccagc
     2941 gggatctccg gcatcttctc gaagatcgtg tcgatgccgc ggaagtacag tccgaaggcc
     3001 ttgtcgaact tgtcgaagtt cgcctcgtcc ttgaccagcg tggtgcgcga caggtagtag
     3061 aactcgtcga gagaaggatt gatcacgtcc ttctgcatcg cctcgagcag caccaggaat
     3121 tccttgatcg agaccgggac cttcgcgtcc ttcagcgtga agaagaagtc aatcagcacg
     3181 ccggcctccg tcgcgcagca cgcgctgcgc cagcttgtcc ttcagatgcg cctcgatctc
     3241 cggccattcg cctgcagtga tgctgtacat gaccgtgtcg cgcaccgtgc cgtcgcggcg
     3301 cagcgcatgg tgacgaatca ccccgtcgcg cctggcgccg agccgctcga tcgcgcgctg
     3361 cgaggcgaga ttgaaattgt cagtgcgaaa gccgaccacc ttgcagccga gggtttcaaa
     3421 cgcatggcgc agcagcatca gcttgcagga ggtgttcaca tgcgtgcgct gccaggactg
     3481 cgcataccag gtgtagccga tctcgacacg gtcgacctcg cgcaggatgt cgtggtagct
     3541 ggtgctgccg atgatcttgc cgcccgacag ctcgcgcacg acgaagggca ggcgggtgcc
     3601 tgcatcgagc atcgacagtg cattgttcac ataggcggcc gtttcgtcgg gcttcggcac
     3661 cgaggtgacg cggatgttcc acagcgcgcc gtcggatgcg gccacggcga ggttgaccgc
     3721 atgcgcgcgc gtcatcggct ccagccggat gccgttgaat tctagggtga cgggctcgag
     3781 cgagatcatc gtttgccgat cagcggttgg cgcgagccat gaaaaccagg cgttcgaaca
     3841 ggtggacgtc ctgttcattc ttcaggagcg cgccatgcag cggcggcacg accgccttcg
     3901 catccttgct gcgcaaggcc tccgggggga tgtcttcggc gagcagcagc ttcagccagt
     3961 ccagcagctc ggaggtcgac ggcttcttct tcaggcctgc gacctcgcgc acttcgtaga
     4021 aggtttgcag tgcctgtgcc agcaaatcct tcttcaggtt cgggtaatgg acgtcgacga
     4081 tctgcgccat cgtgtcgcgg tccgggaact tgatgtaatg gaagaagcag cggcgcagga
     4141 aggcgtcggg cagctccttc tcgttgttcg aggtgatgat caccagcggg cggtgctttg
     4201 ctcggacggt ctcgcgcgtc tcgtacacgt ggaactccat gcgatcgagc tcgcgcagca
     4261 ggtcgttcgg gaattcgatg tcggccttgt cgatctcgtc gatcagcagc acgacctgct
     4321 ggtcggccgt gaacgcctgc cacagcacgc ccttgacgat gtagttgcgg atgtccttga
     4381 ccctgtcatc gccgagttgc gagtcgcgca gccgcgagac ggcgtcgtat tcgtacaggc
     4441 cttgctgcgc cttcgtggtt gacttgatat gccattgcat cagcggcatg ccaagcgcgc
     4501 cggccacttc ctcggccagc atggtcttgc cggtgcccgg ttcgcccttg atcagcagcg
     4561 ggcgctgcag cgtgagggcg gcgttgacgg cgagcttcag gtcttgggtg gctacgtact
     4621 gggcggaacc ttcaaagcgg gtggtcggtt gcatgtcggg gcaggatgga tgcgcggcgc
     4681 gcgcgggttt ttcagtataa gcgagaacgc cttgtcaagc cctgggcggg attgaccgcc
     4741 aagttcttgg ccgtgtaacc acggttaatg tagaattaag cgcttttggt gcgcgcaggc
     4801 aacctcgcgc cagaacctat ccgcaaatgg tcgaaagccg gtcaccttca gccatttgcg
     4861 gataagtttt ttgaattcgt atcactgccc ttagctatca tgaaaaaact ttttgcggtt
     4921 ctcgcgctgg ctggcgcggc caccacggcc tttgcgcagc agacggacat cgtcggcaat
     4981 ccgaaggcgg cggaaaacaa gatcgcgatg tgcatcggct gccatggcat tccgggttac
     5041 agggcgtcgt ttcccgaggt ctaccatgtg ccgatgatcg gcggccagac ccagcgttac
     5101 atcgaggccg cactgcaggc gtacaagcgc ggcgaccgcc gcaatccgag catgcgcggc
     5161 atcgccgccg gcctgtccga ccaggacatc gccgatctcg ccgcctacta cgcccagcag
     5221 aaataagagg acatgatgaa gaagctttcc c
//
LOCUS       DATLGF010000208         6154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_87335,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000208 DATLGF010000000
VERSION     DATLGF010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6154
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87335"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="VM406_06285"
     CDS             <1..1091
                     /locus_tag="VM406_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVL75605.1"
                     /translation="IDEAGAAQRILPKSKQKKTIGKPEIEDIISKIARIPPQSVNQDD
                     RAKLQTIDRDLKNVVFGQDPAIEALGAAIKMARAGLGKTDKPIGSFLFSGPTGVGKTE
                     VAKQLAFILGIELIRFDMSEYMERHAVSRLIGAPPGYVGYDQGGLLTEAITKKPHAVL
                     LLDEIEKAHPDIFNILLQVMDHGTLTDNNGRKADFRNVIIIMTTNAGAEALQKRSIGF
                     TDKKETGDEMAEIKRMFTPEFRNRLDAIISFKALDEEIILRVVDKFLMQLEEQLHEKK
                     VEAIFSDKLRRFLAKKGFDPLMGARPMSRLIQDMIRKALADELLFGRLVNGGKVTVDL
                     DDKDEIKLEFAEGDTAPPPAPEETLEVE"
     gene            complement(1220..2263)
                     /locus_tag="VM406_06290"
     CDS             complement(1220..2263)
                     /locus_tag="VM406_06290"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HVL75606.1"
                     /translation="MSSSLLYALARPLLFSLDPETAHHVALPALVRAEALGLTRLLDR
                     VPTDEREVMGLRFPNPVGLAAGLDKDGACIDGLAALGFGFIEVGTVTPRAQPGNPKPR
                     LFRLPQAHALINRLGFNNGGVDAFVANVQSSRFYQNKEGVLGLNIGKNADTPMERAVD
                     DYVLCLEKVYPYASYVTVNISSPNTKNLRQLQGASELDGLLGQLKQAQSRLADRNGRY
                     VPIALKIAPDVDAEQVKNIADALIRHQFDGVIATNTTISRDAVRGLSHADEAGGLSGP
                     PVFELSNRVIRLLRSELGSDMPIIGVGGILSGEDAREKIAAGASLVQLYTGLIYKGPA
                     LVGECAAALRNTR"
     gene            complement(2311..3075)
                     /locus_tag="VM406_06295"
     CDS             complement(2311..3075)
                     /locus_tag="VM406_06295"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HVL75607.1"
                     /translation="MTHLKDLPFASLQFYATAPYPCSYLEGRQARSQVATPSHLINAD
                     VYSELVRNGFRRSGIFTYRPYCDGCNACIPVRVRADLFSPNRSQRRAWKYHGNLSTWV
                     TTLAYAPEHYDLYLRYQAVRHAGGGMDQDSRDQYAQFLLQSKVNTRLVEFREPDGILR
                     MVSIIDVLGDGLSSVYTFYDPEVQGASYGTYNVLWQIDQARRLGMPHVYLGYWIRDSQ
                     KMAYKANFRPLEAFRGGKWRPWEAQIMPAGNEPRQE"
     gene            complement(3138..3860)
                     /gene="aat"
                     /locus_tag="VM406_06300"
     CDS             complement(3138..3860)
                     /gene="aat"
                     /locus_tag="VM406_06300"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HVL75608.1"
                     /translation="MIPWLEHDSPFPEVETALTEVHGAPGLLAAGADLSPERLLDAYR
                     HGIFPWFSEGQPILWWCTDPRMVLYTDRFTISHSLRKALKKVQRSIRGDGRWEVRFDS
                     AFEDVMRACAEPRTDGAGTWISEELIAGYTALHRRGYAHSSELWFDGRLVGGAYGVCI
                     GRMFYGESMFARQRDASKIALAYLVRFLHANGVEMIDCQQETAHLASLGASPIPRARF
                     LGHLHEAINRPPIENWRPIDII"
     gene            complement(3857..4423)
                     /locus_tag="VM406_06305"
     CDS             complement(3857..4423)
                     /locus_tag="VM406_06305"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HVL75609.1"
                     /translation="MKFCSECAHPVSLAVPPDDNRMRHVCTQCGVIHYQNPKMVIGSI
                     PVWEQDGQARVLLCKRAIEPRYGYWTLPAGFMENNETTSQAAIRETEEEAGARVELHE
                     LFALLNVPHVHQVHMFYRATLLDLNYLAGTESLEVKLFAEHEIPWDDIAFPTVHHTLK
                     YFFTDLARVREGGCFGLHTHDIFKPMRG"
     gene            4569..4645
                     /locus_tag="VM406_06310"
     tRNA            4569..4645
                     /locus_tag="VM406_06310"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4603..4605,aa:Lys,seq:ctt)
     gene            4809..5618
                     /gene="scpB"
                     /locus_tag="VM406_06315"
     CDS             4809..5618
                     /gene="scpB"
                     /locus_tag="VM406_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HVL75610.1"
                     /translation="MNTNEAKRVLETALLCAHEPLSITDMKKLYAEGEGQEHLGTDAI
                     KAMLAELREDWTDRGIELVCLSTGWRFQSRPEMKKYLERLNPEKPPKYTRATLETLAI
                     IAYRQPVTRGDIEEIRGVTVNSQTIKLLEDRGWIETVGHRDVPGRPALFATTRQFLDD
                     LGMTSLDQLPPLQQLSKETLQDGVLPELQAFEGNLQESLEPTPASEVGTAVAMPTGQG
                     SEEGADCLRSSAAQPDQSDSPQRDAARLAAESEAATPAHTDTTNDEPAQPQ"
     gene            5629..>6154
                     /locus_tag="VM406_06320"
     CDS             5629..>6154
                     /locus_tag="VM406_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridylate synthase B"
                     /protein_id="HVL75611.1"
                     /translation="MTDSATPVEAPQIEENSNAEGTADGQPKKKPGKRGVRGPRSLRR
                     ARADRPDGAEAGQATADGGAERRQAEAPAPAQAKRQGNERRKQKAAAQNQRGKQAGQP
                     AGARQPGRKAVNPDDVFAFVTSDTFDTVAADDGKDRNSRAKNVRRDLTAEDDAPKLHK
                     VLAEAGLGSRRDMEE"
BASE COUNT         1236 a         1826 c         2022 g         1070 t
ORIGIN      
        1 tgatcgacga ggcgggcgcg gcgcagcgca ttctgccgaa gtccaagcag aagaagacaa
       61 tcggcaagcc ggagatcgaa gacatcatct ccaagatcgc gcgcattccg ccgcagtccg
      121 tcaatcagga cgaccgcgcc aagctgcaga ccatcgaccg cgacctgaag aatgtcgtgt
      181 tcggacagga tcccgcgatc gaagcgctcg gtgccgcaat caagatggcg cgcgcgggtc
      241 tcggcaagac cgacaagccg atcggttcct tcctgttctc gggtccgact ggcgtgggca
      301 agactgaagt ggcgaagcag ctcgccttca tcctcggcat cgagctgatc cgcttcgaca
      361 tgtcggagta catggagcgc cacgcggtgt cgcgcctgat cggcgcccct ccggggtacg
      421 tcggctacga ccagggcggc ctgctgaccg aggcgatcac gaagaagccg cacgcggtgc
      481 tgctgcttga cgaaatcgag aaagcgcatc cggatatctt caacatcctg ctgcaggtga
      541 tggaccatgg cacgttgacc gacaacaacg gtcgcaaggc cgacttccgc aacgtgatca
      601 tcatcatgac cacgaatgca ggcgcggaag ccttgcagaa gcgttcgatc ggcttcaccg
      661 acaagaagga aaccggcgac gaaatggccg agatcaagcg gatgttcacg cccgagttcc
      721 gcaaccggct cgacgcgatc atcagcttca aggcactcga cgaggaaatc atcctgcgcg
      781 tggtggacaa gttcctgatg caactggaag agcagctgca cgagaagaag gtcgaggcga
      841 tcttcagcga caagctgcgc cgattcctcg cgaagaaggg cttcgatcca ttgatgggcg
      901 cacgtccaat gtcgcgcttg atccaggaca tgatccgcaa ggcgcttgcc gacgagctcc
      961 tgttcggacg tctcgtcaat ggtggcaagg tgactgtcga tctcgacgac aaggacgaaa
     1021 tcaagctcga atttgccgag ggcgataccg caccgccgcc ggcgccggag gaaaccctgg
     1081 aagtcgagta aggctggtcc gcctcaactg caaacccgaa gtgccggatg gcgcttcggg
     1141 ttttttattg cgcgaagagt tattcctgcc ccgtttgcgc gacgattccc ggtgcaatga
     1201 ccggcatggg gcgtgccggt cagcgtgtgt tgcgcaacgc tgccgcgcac tcgcctacga
     1261 gggcgggccc cttgtagatg aggccggtat agagctgtac gagcgatgcg ccggctgcga
     1321 tcttttcgcg cgcgtcttcg ccggaaagaa tgccgcctac gccgatgatc ggcatatcgc
     1381 tgcccagctc gctgcgaagc aggcggatca ctcggttgga cagttcgaag accggggggc
     1441 ccgagagtcc gccagcctcg tccgcatggg acaaaccgcg caccgcgtcg cgggagatgg
     1501 tcgtattcgt cgcgatgacg ccgtcgaact ggtgccggat cagcgcatcg gcgatgtttt
     1561 tcacctgttc cgcatccacg tccggcgcaa ttttcagtgc gatcgggaca tagcggccat
     1621 tgcgatccgc cagccgggac tgcgcctgct tgagctggcc gagcaggccg tcgagttccg
     1681 aggcgccttg cagctggcgc aaattcttgg tgttcggcga ggagatgttg accgtcacat
     1741 aacttgcgta cggatagacc ttctcaaggc acaggacgta gtcgtcgacc gcgcgttcca
     1801 tcggcgtgtc cgcattcttg ccgatgttca ggccgagcac gccttccttg ttctgataga
     1861 agcgcgagga ctggacgttg gcgacgaagg catcgacgcc cccgttgttg aagcccaggc
     1921 ggttgatcag cgcatgcgcc tgtggcagac gaaacaggcg gggtttcggg ttgccgggct
     1981 gcgcgcgcgg agtgaccgtg ccgacttcga tgaagccgaa accgagcgcc gccaagccgt
     2041 caatgcatgc gccatccttg tccaggccgg cagccaggcc aaccgggttg ggaaagcgca
     2101 ggcccatgac ctcccgctcg tctgtcggca cgcggtccag caggcgggtg agtccgagcg
     2161 cttcggcacg cacgagggcc ggcagggcga catggtgtgc ggtttcggga tcgagggaga
     2221 acagcagagg acgagccagt gcgtacagga gggaggagga catgagtggc gaggggtcgg
     2281 cgcagccgac ggacgacgaa aggcggtatt ttactcttgc cgtggttcgt ttccagcagg
     2341 cattatttgc gcttcccagg ggcgccactt tccgccgcgg aacgcctcaa gcggccggaa
     2401 gttggctttg tacgccatct tctggctgtc ccggatccag tagccgagat atacgtgcgg
     2461 catgcccagg cgccgggcct ggtctatttg ccaaaggacg ttgtaggtgc cataggacgc
     2521 gccttgcacc tccgggtcat agaacgtgta taccgacgac agcccgtcgc cgagcacgtc
     2581 gatgatgctc accatgcgca ggattccgtc cggttcccgg aactcgacga ggcgagtatt
     2641 cactttgctc tggagcagaa actgcgcgta ctgatcgcgg ctgtcctggt ccattccgcc
     2701 gccggcgtgc cggaccgcct gatagcgcag gtagagatca taatgctcgg gcgcgtaggc
     2761 tagcgtggtc acccaggtgg atagattgcc gtggtacttc caggcgcggc gctggcttcg
     2821 gttgggcgaa aacaggtcgg cgcgaacgcg caccggtatg caggcattgc agccgtcgca
     2881 gtacggtcgg taggtgaaga tgccgctgcg gcgaaaaccg ttcctgacca gttcgctgta
     2941 gacatcggca ttgatcaggt gtgaaggcgt cgcgacttgc gaacgtgcct ggcgaccctc
     3001 caggtagctg caggggtagg gcgcggtagc gtagaactgc agggaagcaa agggcaggtc
     3061 tttcagatgc gtcataggcc tctcgtggca tgtggcgccg atctgcttac tttacacggt
     3121 tatcgggcgt cgccacgcta gattatgtcg atcgggcgcc agttctcgat cggtggccgg
     3181 ttgatcgctt cgtgcaagtg gcccaggaac cgcgcgcgtg gtatggggct cgctccgagc
     3241 gaagcgaggt gcgcggtctc ctgctgacag tcgatcatct cgacgccgtt cgcatgcagg
     3301 aagcgcacca gatacgcgag agcgatcttg gaggcgtcgc gttgccgggc aaacatcgat
     3361 tcgccgtaga acatcctgcc tatgcacacg ccgtatgcgc cgccgacgag ccggccatcg
     3421 aaccacagtt cggacgagtg cgcgtaaccg cggcgatgca gggccgtata gccggcgatc
     3481 agctcctctg aaatccaggt gccggcgcca tcggtgcggg gctcggcgca ggcgcgcatg
     3541 acgtcctcga aggcggagtc gaagcggact tcccagcgcc cgtcaccacg gatgctgcgc
     3601 tggactttct tcagcgcctt cctgaggctg tgcgagatcg tgaagcggtc ggtgtacagc
     3661 accatgcgcg ggtcggtgca ccaccagagt atcggctgtc cttccgagaa ccaggggaat
     3721 atcccgtggc ggtacgcgtc gagcagacgc tccggggaga ggtcggcgcc tgcggcaagc
     3781 agacctgggg cgccgtgcac ttcggtaagc gcggtctcaa cctcgggaaa cggggaatcg
     3841 tgctccagcc aggggatcat ccgcgcatcg gtttgaagat gtcgtgagtg tggaggccga
     3901 agcatccccc ctcgcgcact cgcgccagat cggtaaagaa gtatttgagc gtgtggtgca
     3961 ccgtgggaaa ggcgatatcg tcccaaggga tctcgtgctc ggcaaacagc ttcacctcca
     4021 ggctctccgt tcccgccagg taattcagat cgagcaatgt ggcgcgatag aacatgtgca
     4081 cctggtggac atgagggacg ttgagcagcg cgaacagctc atgaagttcg acgcgcgctc
     4141 ccgcttcctc ctcggtttcg cgaatcgctg cctgcgacgt ggtttcattg ttttccatga
     4201 agccggccgg cagggtccag taaccatagc gcggctcgat cgcacgcttg cacagcagta
     4261 cgcgggcttg gccatcctgt tcccatacgg gaatcgaacc gatcaccatc ttcgggttct
     4321 gataatgaat tacgccgcat tgcgtgcaga cgtggcgcat ccggttgtca tccggcggga
     4381 cggcgagtga aaccggatgc gcgcattcgg aacagaattt catggagggt gtgcacgaga
     4441 tgcatcgacg ggggagattg ggaagtgtat caccgtcggg tggcgggtcg gccggcgcgg
     4501 ggtcgcgagt ccggcggcgg cacggtttgc gccgacgctt ggaaatggat ataatctcgg
     4561 tttccagacg cggggtggag cagtctggca gctcgtcggg ctcttaaccc gacggtcgca
     4621 ggttcaaatc ctgcccccgc aaccaagtcg tagtccctgc gcgtagtcct tgcgcattcc
     4681 ccgccctcat tagacttcca agaaaacggg cgccggaatg gttccagcgg ttccagatcg
     4741 ggcggcgtag tcagcgcctg tcaattggcg aagattctgc atacgttaca gaagcaatac
     4801 gcacaaccat gaacaccaat gaggccaaga gagtcctcga aacggcgctg ctttgcgcgc
     4861 atgagccgct gtcgattacc gacatgaaga agctctacgc ggaaggcgag gggcaggagc
     4921 acctgggtac cgatgcgatc aaggcgatgc tcgcggaact gcgcgaggat tggaccgaca
     4981 gggggattga gctggtctgc ctctcgaccg ggtggcgctt tcagagccgc ccggaaatga
     5041 agaaatacct ggagcggcta aatcccgaga agccgccgaa atacacgcgc gcgacgctcg
     5101 aaacgctcgc catcattgct taccgtcagc ctgtcacgcg cggcgacatc gaagaaattc
     5161 gcggcgtgac ggtgaactcg cagacaatca agttgctcga agatcgtggc tggattgaaa
     5221 ctgtaggcca tcgcgatgtg ccgggccgcc cggctttgtt cgccacgacc cgccagttcc
     5281 ttgacgatct cggcatgacg tcgctggacc agttgccgcc attgcagcag cttagcaagg
     5341 agacgttgca ggatggcgtt ttgcccgagt tgcaggcgtt cgagggcaac ctgcaggaaa
     5401 gcctcgagcc cactcctgca agcgaagtcg gcacggccgt cgccatgccg accgggcagg
     5461 gcagtgaaga gggggcggat tgtctcagat cttcggctgc ccagccggac caaagcgatt
     5521 caccacaacg cgatgcggcg cgcctggccg cagagagcga ggctgcaacg cccgcgcata
     5581 cagacaccac aaacgatgaa cctgcccaac cgcaataacg cctcagtgat gacagattcc
     5641 gcaacgcccg tcgaagcacc gcagatcgag gaaaacagca acgccgaggg aacggcggat
     5701 ggccagccga aaaagaagcc gggcaagcgc ggtgtgcgcg ggccgcgcag cttgcggcgc
     5761 gcccgtgccg atcgaccgga tggtgcggaa gccggacagg caaccgcgga tggtggggcg
     5821 gaaaggcggc aggccgaagc tcccgctccc gctcaggcga agcgccaggg caacgagcgc
     5881 aggaaacaga aggcggctgc acagaaccag cgcggcaagc aggccggtca gccggccggc
     5941 gcgcgccagc cgggacgcaa ggcggtaaac cctgatgacg tgttcgcctt cgtgacttcc
     6001 gatacgttcg acaccgtcgc cgccgatgac ggcaaggatc gcaacagccg ggcgaagaac
     6061 gtcaggcgcg acctgaccgc ggaggacgac gcacccaagc tgcacaaggt gcttgccgag
     6121 gccggactgg gttcgcgccg cgacatggaa gaac
//
LOCUS       DATLGF010000209         2647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_165392, whole genome shotgun sequence.
ACCESSION   DATLGF010000209 DATLGF010000000
VERSION     DATLGF010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165392"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VM406_06325"
     CDS             <1..239
                     /locus_tag="VM406_06325"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVL75612.1"
                     /translation="GTFRGLEGVREKVRLLAEQLPRGRWTYRTVLADGEMGFLEWSAE
                     AENGARVEDGADSYLIQNGRIRAMTIHYTVLPPP"
     gene            484..822
                     /locus_tag="VM406_06330"
     CDS             484..822
                     /locus_tag="VM406_06330"
                     /inference="COORDINATES: protein motif:HMM:NF019401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HVL75613.1"
                     /translation="MVSFRDVAESAGLLIDSVGVAVIVIGLVIATARYLVEGRKLADG
                     YKRYRQDLGRGILLGLEFLVAADIIRTVAVAPTVEGVLVLGIIVLIRTFLSVSLQVEL
                     EGRWPWQAKE"
     gene            complement(886..1431)
                     /locus_tag="VM406_06335"
     CDS             complement(886..1431)
                     /locus_tag="VM406_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75614.1"
                     /translation="MALVPVSEAAGLTRVDPATLYRDVDSGRLQATRTADGDILLDTG
                     ELKRIYGDADSGPDASLSAEQVKIAMLEERTRALERALALEAELRKVKDQVANELRAR
                     LADKETVIRTLQNRVMFLETDRPDPIPTLAVDGVEEEAENAAPVTTSDESRQIGDERQ
                     LVSGGWWSRLLGRRGGSSLSG"
     gene            complement(1524..>2647)
                     /locus_tag="VM406_06340"
     CDS             complement(1524..>2647)
                     /locus_tag="VM406_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HVL75615.1"
                     /translation="ELLAAARRPLVLLGGSGWNPEACADMRRFIEASALPAACAFRFQ
                     DLLDNRHPNYIGDVGIGINPKLAARIQNADLILAIGPRLGEMTTSGYTLLEAPCPHQK
                     LVHIHSGAEELGRVYQGELMINSGMPQIASALAAMEPVDSSAWRSSVEEARAELVAWQ
                     AEPPLFKQTPARLNLWQMVQVLKQRLPADAILTNGAGNFATWAHRFWPYAGFRTQLAP
                     TSGAMGYGVPAAVAAAIATRNARTVVNFAGDGDYMMNGQELATAVQYGAGVLFIVFNN
                     GTYGTIRMHQERHFPARVSGTDLRNPDFAALARAYGAHGEMVDTTEAFGPALERALAH
                     IQATGLPALIELQYEANLLTPNASLEQIRAAGQKASQES"
BASE COUNT          397 a          804 c          939 g          507 t
ORIGIN      
        1 atggaacctt ccgtgggctt gagggggttc gggagaaggt gcgcttgctc gcggagcagc
       61 tgccgcgcgg ccgctggacc taccgtacgg tactggccga tggcgagatg ggctttctgg
      121 agtggagcgc cgaggcggag aatggcgccc gcgtcgagga tggagccgac tcttacctga
      181 tccagaacgg acgcatccgc gcgatgacta ttcactacac ggtgttgccg ccgccgtgag
      241 gcgtgcaact ggccgagacg gccgaccggc ccgcacactc gctgcacacc cgtgcatgcg
      301 cgagccgggg tcgactaccg ctttcctgct ccaaaccgga ttgcgcccct ggcgaatcag
      361 tccctcatcg acgagatgac gcgtcatgag gaatgagacc cgcgcggaac aacggtcttt
      421 gtcacggcga tccgccagag ccctctttcc aggccgccgc ctggtgctgt cgcggaggtc
      481 gatatggtgt ccttcaggga tgtggctgaa agcgccggtt tgctgattga cagcgtcggc
      541 gttgcggtca tcgtgatcgg gctggtgatt gctactgcgc gctatcttgt cgaaggcaga
      601 aagctggccg acggatacaa gcgatatcgg caggacctgg ggaggggcat actcctcgga
      661 ctggagttcc tcgtcgcggc cgacatcatc cgcacggttg cagtggcgcc tacagttgaa
      721 ggggtgctgg tgctggggat cattgtgctg atcaggactt tcctgagcgt gtccctgcag
      781 gtggaactcg aagggcgttg gccctggcag gcgaaggagt aggcagggtg tacgcccgcg
      841 ctgcttgcct tccggctttg cgggccgatg tgtgcaatgc gggatctacc cggacaagga
      901 cgacccgccg cgccggccca gcaggcgcga ccaccagccg ccgctcacca gttgccgctc
      961 gtcgccgatc tgccggctct cgtcggaagt cgtgaccggc gcggcattct ccgcttcctc
     1021 ctccacgcca tcgacggcaa gcgtcgggat cggatcgggc cggtcggtct caaggaacat
     1081 caccctgttt tgcaaggtgc ggatcacggt ttccttgtcg gcaaggcggg cgcgcagctc
     1141 gttggcgacc tggtccttga ccttgcgcag ttccgcttcc agcgccagtg cgcgttccaa
     1201 tgcgcgcgtg cgctcctcca gcatcgcgat tttgacttgc tcagcggaca gggaggcgtc
     1261 cggtccgctg tcggcgtcgc cgtagatgcg cttgagctcg ccggtgtcga gcaggatgtc
     1321 gccgtcggcg gtgcgcgtcg cctgcaggcg gccgctgtcg acgtcccggt acagggtggc
     1381 cgggtccacg cgggtgaggc ctgcagcttc gctgacgggt acgagtgcca tggagacggt
     1441 cagtagcagt tggcgggatg ctctactgct acagacactc tgcgccggaa ataattcggc
     1501 gcggcgttgc aatggcgggc gggtcagctt tcctgactgg ccttctggcc cgccgcacgg
     1561 atctgttcca ggctcgcgtt cggcgtcagc aggtttgctt cgtactgcag ttcgatgagt
     1621 gccggcaacc cggttgcctg gatgtgcgcg agtgcgcgtt ccagcgccgg gccgaaggct
     1681 tcggtcgtat ccaccatttc accatgggcg ccgtatgcgc gtgcgagcgc ggcgaagtcg
     1741 ggattgcgca ggtcggtgcc ggatacgcgc gccgggaaat ggcgttcctg gtgcatgcgt
     1801 atcgtgccgt aggtgccatt gttgaacacg atgaacagca cgccggcgcc gtactgcacg
     1861 gcggtggcga gctcttgccc gttcatcatg tagtcgccgt cgcccgcgaa gttgacgacg
     1921 gtgcgggcgt tgcgggtggc aatcgccgcg gccaccgccg ccggcacgcc gtagcccatt
     1981 gcgccggagg tcggcgcgag ctgggtgcgg aatccggcgt acggccagaa tcgatgtgcc
     2041 caggtcgcga agttgccggc accgttcgtg aggatggcgt cggcgggaag ccgctgcttc
     2101 aggacctgga ccatctgcca caggttcagg cgcgccgggg tctgcttgaa cagcggcggt
     2161 tcggcctgcc atgcgacgag ttcggcgcgc gcttcttcga cgcttgagcg ccaggcgctg
     2221 ctgtcgaccg gttccatggc ggcaagtgca gatgcaattt gcggcatgcc gctgttgatc
     2281 atcagctcac cctggtacac gcgtccaagt tcttctgcgc cgctgtggat atgcaccagc
     2341 ttctggtgcg gacagggcgc ttccagcagg gtgtaaccgc tggtggtcat ttcgcccagg
     2401 cgcgggccga ttgcgaggat caggtcggca ttctggatgc gcgcggcgag tttcgggttg
     2461 atgccgatgc cgacatcgcc gatgtaattc ggatgacggt tgtcgagcag gtcctggaag
     2521 cggaacgcgc acgcggcggg cagggcgctg gcttcgatga agcggcgcat gtcggcacag
     2581 gcttccgggt tccagcccga gccgccgagc aggacgagcg ggcgccgggc ggcggcgagc
     2641 agttcgc
//
LOCUS       DATLGF010000210         2393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_165539, whole genome shotgun sequence.
ACCESSION   DATLGF010000210 DATLGF010000000
VERSION     DATLGF010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165539"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VM406_06345"
     CDS             complement(<1..712)
                     /locus_tag="VM406_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVL75616.1"
                     /translation="MQTLTSKLHHPLSPIATAVVLVLLLLLPWIAEAMDEPFYISVAS
                     RLLIFAIAATSLNLVLGFGGMVSLGHAAYFGAGAYTIGILMQHDVLSGWIAWPAAVAV
                     GALLAAAIGAVSLRTKGVYFIMITLAFAQMMFYLFISMRDYGGDDGMSLYQRSALGFD
                     ISGDTPFYYVVLALAAAAFYLVYRVVNSRFGRTVQAIRENESRMEAIGYPVFRYKLVC
                     FIIGGALAALAGALLANQN"
     gene            complement(717..1637)
                     /locus_tag="VM406_06350"
     CDS             complement(717..1637)
                     /locus_tag="VM406_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVL75617.1"
                     /translation="MDPTLILENLLNGLQFGLMLFLLAAGLTLVFGIMDFINLAHGSL
                     YMAGAYFAATFTGITGSFWLGLLLAIGATAILGVILEMSVFRRLYQRDHLSQVLATFA
                     LILIFNEGARMIWGAQPLMLNTPPELARPVELLPGFYYSSFRLFIIGAGIAVAALLYW
                     IVVKTRIGMLVRAGASNREMATAMGVNIKLVFTLLFAFGAALCAVAGSLLGTILAVQV
                     GMGESILILAFVVIVIGGIGSVRGALVGALLVGIVDTASRTLLPVFLRTLLPPDIAAN
                     LGPALASILIYVLMAAVLFFKPEGLFPARG"
     gene            1815..>2393
                     /locus_tag="VM406_06355"
     CDS             1815..>2393
                     /locus_tag="VM406_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVL75618.1"
                     /translation="MTATAHVDTFVRDNLPPRELWPELRFDLPELQYPSHLNCAAELL
                     NEDHGERTAILWPSGRWTYAELGAKVDRIAHVLVDDLGLVPGNRVLLRGANGPMLAAA
                     VLAVWKAGCVAVPTMPLLRAPELRAIINKAQVNAALCAASLCEELDRACEAGGPLKHV
                     VHFGAPQEDLERRMAAKGEPFVAVSTASDDPCL"
BASE COUNT          454 a          752 c          829 g          358 t
ORIGIN      
        1 tgttctggtt ggccagcaga gcaccggcaa gcgcggccag cgcgccgccg atgatgaagc
       61 acaccagctt gtagcggaac accgggtagc cgatcgcctc catccggctt tcgttctcgc
      121 gaatcgcctg cacggtgcgg ccgaagcgcg agttgacgac gcggtagacg aggtagaagg
      181 ccgcggcggc gagcgccagc acgacgtaat agaagggggt gtcgccggag atgtcgaagc
      241 cgagggccga gcgctggtac aggctcatgc cgtcgtcccc gccgtagtcg cgcatcgaga
      301 tgaacaggta gaacatcatc tgtgcgaagg ccagggtgat catgatgaag tacacgccct
      361 tcgtgcgcag cgataccgcg ccgatggctg cggccagcag cgcgccgacc gcaacggcgg
      421 ccggccaggc gatccagccg gacaggacat cgtgctgcat caggatgccg atcgtgtagg
      481 cgccggcgcc gaagtaggcg gcatgcccga gactgaccat gccgccgaag ccgagcacca
      541 ggttcaggct ggtggcggcg atcgcgaaga tcagcaggcg gctggcgacg ctgatgtaga
      601 acggttcgtc cattgcctcg gcgatccacg gcagcaggag cagcagcacg agcacgacgg
      661 ccgtggctat gggggagagc ggatggtgga gcttgctggt cagggtttgc atgcgactat
      721 cctcgcgccg ggaacaagcc ttccggcttg aagaacagga cggcggccat cagcacgtag
      781 atcaggatcg aggccagcgc cggtccgagg ttggccgcga tgtccggcgg cagcagcgtg
      841 cgcaggaata ccggcagcag cgtgcggctg gcggtgtcca cgatgcccac cagcagcgcg
      901 ccgaccagtg cgccgcgcac cgagccgatg ccgccgatca cgatcaccac gaacgcgagg
      961 atcaggatgc tctcgcccat gccgacctgc accgcgagga tggtgccgag cagcgagccg
     1021 gcgaccgcgc acagcgccgc gccgaacgcg aacagcagcg taaacaccag cttgatgttg
     1081 acgcccatcg cggtcgccat ctcgcggttc gatgcgccgg cgcgcaccag catgccgatg
     1141 cgcgtcttca cgacgatcca gtagagcagg gcggcgaccg cgatgccggc gccgatgatg
     1201 aacaggcgga acgacgagta atagaagccg ggcagaagct ccaccggcct ggcgagctca
     1261 ggcggcgtgt tcagcatcag cggctgggcg ccccagatca tgcgcgcgcc ttcgttgaag
     1321 atcaggatca gcgcgaacgt ggcgagcacc tgcgacagat ggtcgcgctg gtagaggcgc
     1381 cggaacacgc tcatttcgag gatcacgccg aggatggcgg tcgcgccgat ggccagcagc
     1441 agccccagcc agaacgagcc ggtgatcccg gtgaaggtgg cggcgaagta ggcgccggcc
     1501 atgtagaggg agccgtgcgc gaggttgatg aagtccatga tcccgaacac cagcgtcagg
     1561 ccggcggcga gcaggaacag catcagcccg aactgcaggc cgttgaggag gttttcgagg
     1621 atcagtgtcg ggtccatcgc cgggaaatgc cgtgcagtaa ttcggtcagg cctgaagaat
     1681 acatgatcgc gggttgaccg attcggcatc ggtcaacgaa aggatacttg aaatcgaaat
     1741 attttagggc taaagtacat tccgcttttc ggcaacaccg tgctgctcgg cctcgcttcc
     1801 cttccggagc tttcatgact gctacagccc atgtcgatac cttcgtccgc gacaacctgc
     1861 cgccgcgcga gctgtggccc gagctgcgct tcgacctgcc tgaactgcaa tacccgtctc
     1921 acctgaattg tgccgccgag ctgctgaacg aggaccatgg cgagcgcacg gccatcctct
     1981 ggccttcggg ccgctggacc tatgccgaac tgggggcgaa ggtcgaccgc atcgcccatg
     2041 tgctggtgga cgaccttggg ctcgtgcccg gcaaccgcgt gctgctgcgc ggcgcgaacg
     2101 gcccgatgct ggccgcagcg gtgctcgccg tgtggaaggc cggctgcgtg gcggtgccga
     2161 cgatgccctt gctgcgcgcg ccggaactgc gcgccatcat caacaaggcg caggtcaacg
     2221 cggccttgtg cgccgcatca ttgtgcgagg agctcgaccg cgcgtgcgag gcgggcggac
     2281 cgctcaagca cgtggtccat ttcggcgcgc cgcaggagga cctggagcgg cgcatggcgg
     2341 ccaagggcga gcccttcgtc gcggtgtcga ccgcatccga cgatccctgc ctg
//
LOCUS       DATLGF010000211        11369 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_40944,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000211 DATLGF010000000
VERSION     DATLGF010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 11369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11369
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40944"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VM406_06360"
     CDS             <1..570
                     /locus_tag="VM406_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family cobalamin biosynthesis protein"
                     /protein_id="HVL75619.1"
                     /translation="AARIVSRDTGGASEADIARAAAESTLENGNDAVFGTLFWFIVAG
                     GPGALLFRLANTLDAMWGYRNARFHAFGWAAARIDDALNLIPARLTALSYALLGNTRC
                     ALACWRSQASAWSSPNAGPVMASGAGALTIVLGGPAVYDGVIEARPPLGTGAPPRASD
                     IARAWRLVARAVALWLAVAVLIATGRGFA"
     gene            567..1565
                     /gene="cobD"
                     /locus_tag="VM406_06365"
     CDS             567..1565
                     /gene="cobD"
                     /locus_tag="VM406_06365"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HVL75620.1"
                     /translation="MIAHGGNLREAATRYGRPLDDWIDLSTGINPNGYPVPTLGPDVW
                     HRLPEPDPELVAAACACYGAPALLPVAGTQAAIQALPRLRRPGRVVVSSPSYAEHAHC
                     WAGAGHAVHEARFDELEGVLDDCDVMVVCNPNNPTGATVEAQRLLDWSGRLANRGAWL
                     VVDEAFGEVAPALTVAPHAHRPGLIVLRSVGKFFGLAGLRLGFVAAHDALLDALSTCL
                     GPWSVSTPAQQIGKAALSDRAWQGRTREALLRDGMRLHTLLARHGIATTGTALFRYWP
                     YAQAAAFQQHMARHGVWVRRFDAPAQGVRLGLPGSEAGWQRLETALAAWARMEHAA"
     gene            1562..2446
                     /locus_tag="VM406_06370"
     CDS             1562..2446
                     /locus_tag="VM406_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HVL75621.1"
                     /translation="MKRLAMTALLACAAQATAAISVKDDRGETVTLARPAQRVITLSP
                     HTTELVFAAGAGERIIGTVRHSEYPEAAKAIARVGDNKLIDLERVVAMQPDLLVVWLH
                     NVAERQVEALRRLGIPMFYSDPRRLDDIPDTLERLGLLLGTGAHAQREARQFRERMAQ
                     LKEQHRQRPVVRVFYQLWDAPLYTLNDKSIASDVLRTCGAENVFGALSLPAPVVSVEA
                     VLLEKPDAIVAAAGGKRSSDGLGHWRAYTSLPAVRHGNLFAVNSDLINRPGLRILDGA
                     ALVCEQMERARRELERRP"
     gene            2443..3240
                     /locus_tag="VM406_06375"
     CDS             2443..3240
                     /locus_tag="VM406_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL75622.1"
                     /translation="MSHYRRIACLSTEAVETLYALGAQDSIAGISGFTVRPPEARRDK
                     PKISGFSSSKIERILAVEPDLAIGFSDMQADICAALAKHGVEVMLFNQRDIAGILRFV
                     RVLAALVERRQEGERLLARLQVQIDAVRASVRAPAPRIYFEEWNEPMISGIGWVSECI
                     ALAGGEDVFAELAAHAGAKERIIADPMEVVRRAPDIIIGSWCGKKFRPESVAARPGWD
                     AVPAVRDGLVEEIKSADILSPGPSVILHGLPQIAALVARWHAMREAR"
     gene            3261..4724
                     /locus_tag="VM406_06380"
     CDS             3261..4724
                     /locus_tag="VM406_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HVL75623.1"
                     /translation="MVQGTTSDAGKSTVVAALCRLLAREGVRVAPFKPQNMALNSAVT
                     EDGGEIGRAQALQALAAGVPPHTDMNPVLLKPSSDTGAQVIIHGQVRADMNARDYHAY
                     KTTAMQAVLASYRRLRERFDVIIVEGAGSPAEINLRDRDIANMGFAEAVDCPVILVAD
                     IDRGGVFAHIVGTLACLSESERARIVGFVINRFRGDISLLQPGVDWLEQQTGKPVLAV
                     LPYLHGLHLDAEDAIAKDQAEQGGFRVIVPALPRISNHTDFDYLRAHPEVDLRFIGPH
                     QAIPPADLIILPGSKNTRADLDWLKTQGWREAITRHLRYGGKLIGICGGFQMLGTLVH
                     DPNGVEGAPGTSAGLGLLDIETTLTTQKRLAQASGRCLFADASVRGYEIHMGRSSGAG
                     LARPAFEIEGRAEGALSGDGQVLGTYLHGLFDAPDAGRALLRWAGLESDAPTDLSRLR
                     EQAIDRIADAALPLLAGLDRFDQFGVAAQSDLVREQT"
     gene            complement(4812..6044)
                     /locus_tag="VM406_06385"
     CDS             complement(4812..6044)
                     /locus_tag="VM406_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HVL75624.1"
                     /translation="MSGTELADGPLFEKWERNGLELANRLAVAPMTRVSATPLGVPTP
                     AMVRYYTRFARGGFGLVITEGIYTDRAYAQGYANQPGLTDAEQLGGWRSVVDAVHAAG
                     GKIVAQLMHAGALAQGNRFRGEPAGPSAVAPKGLQMPFYGGQGPYRTPLAMSPADIRA
                     AIDGFASAANLARAAGFDGVEIHGANGYLLDQFLTGYTNRRSDDWGGTIERRVRLCVG
                     VAQAVRAAVGTHFPVGMRISQAKVNDFEHKWPEGEDGARIVFGLLADVGLDFLHVTEF
                     EAWKAAFGDTGPSLVALARRYAPGVTMIANGSLHAPQRALQLLEDGADIIALGRGALA
                     NPDWPNRLAGGEPLAGFDRELLAPLADIKPSELAQRGVDGRREEIGGPPGRQAGSDPI
                     REGRAQAGPAERKDPCHR"
     gene            complement(6041..6439)
                     /locus_tag="VM406_06390"
     CDS             complement(6041..6439)
                     /locus_tag="VM406_06390"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HVL75625.1"
                     /translation="MKLEQVKTNPDPYEPYLLSQAIRAGQLLFVSGQAAYGEDGGIVG
                     KGDFARQADQAFRNLERALKAGGSGMDRVVKVTIFLTSMDHFSTIVEMRRKWFAKPYP
                     ADTIVEISALYSPDAMIEIEAIALAVEGEQ"
     gene            6567..7469
                     /locus_tag="VM406_06395"
     CDS             6567..7469
                     /locus_tag="VM406_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013954034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVL75626.1"
                     /translation="MRKLPPLSALRVFEAAARRLSFRLAAEELHVTPTAVSHQIRQLE
                     DWMGVRLFERGTRKVSLTGAGARLFPPLRDGLDSFERAVDEVRRSQAAKVATLSSTVA
                     FMARRLAPLAGSFRSAYPDWTLRLDASNDPVDLDGEADAAIRYGRGHYPGLVVVPLLA
                     DRFAPVCTPRLAAAARKGLRGATLIHFEWGPIARADEQAPTWRRWLEAAGMTGVDAEA
                     GLSFTDEIHAVHATLAGQGIGMLSLNLVADEIASGLLVQPFDVAIGNYRYDLVYSPRA
                     AARPATVVLVDWVRRAFGPVRETG"
     gene            complement(7474..7962)
                     /locus_tag="VM406_06400"
     CDS             complement(7474..7962)
                     /locus_tag="VM406_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HVL75627.1"
                     /translation="MNDTTSHAALPFALARNAFGRLELVRADGAHFNEVVPVRAFPIQ
                     APDEGIALVCPDGREAAWIERLDALPPAQRQLVEEALAVREFMPRIRKIARVTSYATP
                     STWHVLTDRGAAAFVLRGEEDIRRIGPQMLLISDSHGIQFLVPDMLALDRASLKILDR
                     FL"
     gene            complement(7964..10126)
                     /locus_tag="VM406_06405"
     CDS             complement(7964..10126)
                     /locus_tag="VM406_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL75628.1"
                     /translation="MEIDLDARLAFGSGLLAVTDRRVLAQAPGESTLADWDYREGLAL
                     RHADHAGVGHLELVDANGRLVAWHFTLGHNLHAMRLLDQFVLRQTGRLGGKPAGEPER
                     NACPGCKAPLEPDQDACPVCTKVIHTPPSTWTLFRLWRFARPYRLQLLAGFILTLLGT
                     AASLVPPYLTMPLMDEILIPYQNGTQIDPSLVALYMGGLLGAALLAWILSWARTYILA
                     LVSERIGADLRTTTYEHLLRLSHEYFGGKRTGDLIARIGSETDRICVFLSLHLLDFAT
                     DVLMLVMTATILFSINPWLALVTLLPLPLIAWMIHVVRDRLRTGFEKIDRVWGEVTNV
                     LADTIPGIRVVKAFAQERREADRFRATNRHNLAVNDRLNKTWSVFSPTVSLLTELGLL
                     VVWAFGIWQVAHGDITVGVLTAFVTYISRFYGRLDAMSRIVSVTQKSASAAKRIFDIL
                     DHVSSVPEPARPVPCAQIKGRIELVDVAFRYGNRSVARGINLDIAPGEMLGLVGHSGS
                     GKSTLVNLICRFYDVAEGAIRVDGTDIRSFAIADYRRHIGLVLQEPFLFFGTIAENIA
                     YGKPDASRAEIVAAARAAHAHEFILRLPQGYDSMVGERGQGLSGGERQRISIARALLI
                     DPRILILDEATASVDSETEKEIQKALDNLVRGRTTIAIAHRLSTLQRADRLVVLDRGQ
                     VIEQGAHDELMAREGAYYRLYQAQARAHAEAAVPASED"
     gene            10438..>11369
                     /locus_tag="VM406_06410"
     CDS             10438..>11369
                     /locus_tag="VM406_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HVL75629.1"
                     /translation="MTHKDLHFLRVTHLRGPNIWTYRPVLEAWVDIGALEDAPSNTIA
                     GLYERLSAMLPGLIEHRCGVGRRGGFLERLKEGTWAGHILEHVVLELQTMAGMKTGFG
                     KARETSVRGVYKVVFRTRHEQVGRAALDAGRALLLAAIENRPYDVAAAVARLRGMVDD
                     LCLGPSTAHIVDAAVERGIPFIRLTEGNLVQLGHGVRQRRIWTAETDATGAIAEGIAG
                     DKDLTKSLLRACGVPVPEGATVDSAEAAWEAAQEIGLPVALKPQDGNHGRGVSLDLMS
                     RDEVIAAYELARAEGDGGVIVEQFIAGNEHRLLVV"
BASE COUNT         1795 a         3753 c         3965 g         1856 t
ORIGIN      
        1 gccgcgcgca tcgtcagccg cgacaccggc ggggccagcg aggcggatat cgcaagggcc
       61 gcagccgaat ccacgctgga aaacggcaac gatgcggtgt tcggcacgct gttctggttc
      121 attgtcgccg gcgggcccgg cgcgctgctg ttccggctgg ccaatacgct ggacgccatg
      181 tggggctacc gcaacgcgcg cttccatgcg ttcggctggg ctgccgcgcg catcgacgat
      241 gcgctaaacc tgattccggc gcggctgacg gcgctgtctt acgcactgct tggcaatacc
      301 cgctgtgcgc ttgcctgctg gcgctcgcag gcaagcgcct ggagcagccc gaacgccggg
      361 ccggtgatgg cgtccggcgc gggggcgctg accatcgtcc tgggcggacc ggccgtctat
      421 gacggcgtca ttgaagcgcg gccgcctctc ggcaccggcg cgccgccgcg ggcgtccgac
      481 atcgcgcgcg cctggcgcct cgttgcgcgt gcggtcgcgc tgtggctggc agtggctgtg
      541 cttatcgcaa caggaagggg atttgcatga tcgctcacgg gggcaacctg cgggaggcgg
      601 cgacgcgtta cggccggccg cttgatgact ggatcgatct ctcgaccggc atcaatccga
      661 atggctaccc ggtgccgacc ctgggcccgg acgtctggca ccggctcccc gagcccgatc
      721 cggaactggt ggccgccgcg tgcgcatgct acggcgcgcc ggcgttgctg ccggtggccg
      781 gcacgcaggc ggcaatccag gccttgccgc ggctgcggcg gccgggcagg gtggtcgttt
      841 cctcgccgtc ctatgccgag catgcgcact gctgggcggg cgcagggcat gcggtgcacg
      901 aagcgcgttt cgacgaactc gagggcgtgc tggacgactg cgacgtcatg gtggtctgca
      961 atcccaacaa tccgaccggc gcgacggtag aggcgcaacg cctgctcgac tggtccgggc
     1021 gccttgcaaa ccggggcgcg tggctggtcg tcgacgaggc attcggggag gtcgccccgg
     1081 cacttacggt ggcgccgcac gcgcaccggc ccggcctgat cgtgttgcgc tcggtcggca
     1141 aattctttgg tctggcgggc ctgcgccttg gctttgtcgc cgcgcacgac gcgctgctcg
     1201 atgcgctttc aacctgtctc ggcccctggt cggtcagcac cccggcgcag cagatcggca
     1261 aggcggcgct gtccgaccgc gcgtggcagg gccgcacgcg tgaggccctg ctgcgcgacg
     1321 gcatgcgcct gcacacgctg ctggcacggc atggcatcgc cacaacgggc accgcgctgt
     1381 tccgttactg gccgtacgcg caggcggcgg cgtttcagca gcacatggcc cggcacggcg
     1441 tatgggtacg ccggttcgat gcgccggcgc aaggcgttcg cctgggcctg ccgggtagcg
     1501 aggccggatg gcaaagactg gagaccgcgc tggcggcttg ggcgcggatg gagcacgcgg
     1561 catgaaacga ctggcaatga cggccttgct ggcctgcgcg gcgcaggcga cggcagcgat
     1621 ctcggtcaag gacgaccgcg gcgagacggt cacgctggcc cgtccggcgc agcgcgtgat
     1681 cacgctgtcc ccgcacacga ccgaactcgt gttcgcggcc ggcgcgggcg agcgcatcat
     1741 cggcacggtg cggcacagcg aatatccgga ggccgcaaaa gccattgcgc gcgttggcga
     1801 caacaagctg atcgacctgg agcgggtggt cgcgatgcag ccggatctgc ttgtggtctg
     1861 gctgcacaac gtcgccgagc gccaggtcga agccctgcgc aggctcggca ttccgatgtt
     1921 ctacagcgat ccgcgccggc tggacgacat tcccgatacg ctcgaacgcc tcggcctgct
     1981 gctcggcacc ggcgcccatg cacagcggga ggcaaggcag ttccgcgagc gcatggcgca
     2041 gctgaaggag cagcatcggc agcgaccggt ggtgcgggtg ttctaccagc tctgggatgc
     2101 gccgctctac acgctcaacg acaagagcat cgccagcgac gtgttgcgca cctgcggggc
     2161 cgagaacgtg ttcggcgcgc tgtcgctgcc ggccccggtc gtgagcgtgg aagcggtgct
     2221 gctggaaaaa ccggatgcaa tcgtcgccgc cgccggcggc aagcggtcca gcgacggcct
     2281 cggtcactgg agggcgtaca cgagcctgcc ggcggtgcgc catggcaacc tgtttgccgt
     2341 gaattcagac ctgatcaacc ggccgggtct gcgcatactc gacggcgcgg cgcttgtctg
     2401 cgagcagatg gagcgcgccc ggcgcgaact ggagcgacgt ccatgagcca ctaccgccgc
     2461 atcgcctgcc tgtccaccga agccgtcgag accctgtatg cgctcggcgc gcaggacagc
     2521 attgccggca tttccggctt caccgtgcgt ccgccagagg cgcgccggga caagccgaag
     2581 atcagcgggt tctcctcgtc gaaaatcgaa cgcatcctcg ccgtcgagcc cgatctggcg
     2641 atcggctttt ccgacatgca ggccgacatc tgcgccgcac ttgcgaagca cggcgtggag
     2701 gtcatgctgt tcaaccagcg cgacatcgcc ggcatcctgc gcttcgtgcg ggtgctggcg
     2761 gcactggtgg aacgccggca ggagggtgaa cgcctgctcg cgcggctaca ggtgcaaatc
     2821 gatgcggttc gtgcgagcgt gcgcgcaccg gcgccgcgca tctacttcga ggaatggaac
     2881 gagccgatga tctcgggcat cggctgggtg tcggaatgca ttgcgctcgc tggcggcgag
     2941 gacgtgttcg ccgagctcgc cgcgcatgcg ggcgcgaagg aacgcatcat cgccgatccg
     3001 atggaagtgg tgcggcgcgc gccggacatc atcatcggtt cctggtgcgg caagaaattc
     3061 cgccccgaga gcgtggccgc caggccgggt tgggatgcgg tgccggcagt gcgcgacggg
     3121 ctggtggaag agatcaagtc ggccgacatc ctttcgcccg gaccctccgt catcctgcac
     3181 ggcctgccgc agatcgcggc gctggtcgcg cgctggcacg cgatgcggga ggcgcggtga
     3241 attttccgtt tcatacgctg atggtgcagg gcacgacctc ggacgccggc aagagcacgg
     3301 tggttgccgc gctgtgccgg ctgctggcgc gcgaaggcgt gcgggtggcg ccgttcaagc
     3361 cgcagaacat ggcgctcaac agcgcggtga ccgaggacgg cggcgagatc gggcgggccc
     3421 aggccttgca ggcgctggcc gccggcgtgc cgccgcatac cgacatgaac ccggtgctgc
     3481 tcaagccttc gtccgacacc ggtgcgcaag tcatcatcca tggccaggtg cgtgcggaca
     3541 tgaacgcgcg cgactaccac gcctacaaga ccaccgcgat gcaggcggtg ctggcgtcct
     3601 accggcgcct gcgcgagcgc ttcgacgtca tcatcgtcga aggcgcgggc agccccgccg
     3661 agatcaacct gcgcgatcgc gacatcgcca acatgggctt tgccgaagcg gtcgattgtc
     3721 cggtcatcct ggtggccgac atcgatcgcg gcggcgtgtt cgcccatatc gtcggcacgc
     3781 tcgcctgcct gtccgagagc gaacgcgcgc gcatcgtcgg cttcgtgatc aaccgattcc
     3841 gcggcgatat ctcgctgctg cagccgggcg tggactggct cgagcagcag accggcaagc
     3901 cggtgctcgc ggtgctgccc tacctgcacg gcctgcacct ggacgccgag gatgcgattg
     3961 cgaaggacca ggcggagcag ggcggtttcc gggtgatcgt gccggcgctg ccgcgcatca
     4021 gcaatcacac ggatttcgat tacctgcgcg cgcacccgga ggtggatctg cgcttcatcg
     4081 ggccgcacca ggctatcccg ccggccgacc tgatcattct tccgggcagc aagaacaccc
     4141 gtgccgacct cgactggctc aagacgcagg gatggcgcga ggccatcacc cgccacctgc
     4201 gctacggcgg caagctgatc ggcatctgcg gcggcttcca gatgctcgga acccttgtgc
     4261 atgaccccaa cggcgtcgaa ggggcacccg gcacatcggc cggtctcggc ctgctcgaca
     4321 tcgagacgac gctgacgacg caaaagcggc tggcgcaggc aagcggacgc tgcctgttcg
     4381 ccgatgcgtc agtgcgcggc tacgagatcc acatgggcag gtcgtcgggc gccgggcttg
     4441 cgcggccggc gttcgagatc gaggggaggg ccgagggcgc gctttccggc gacgggcagg
     4501 tgctcggcac ttacctgcac ggcctgttcg atgcgccgga tgcgggccgg gccttgctgc
     4561 gctgggccgg actggagagc gacgcgccga cggacctgag ccggctgcgc gagcaggcga
     4621 tcgatcgtat cgcggacgcc gcgctcccgc tgctggcggg gctggaccgg ttcgaccagt
     4681 tcggagtagc cgcacaatcg gacctcgtgc gtgagcaaac gtagcggctc gcacatcgcc
     4741 tgatcgagat ttccaggatc gatgcgctgc tcacgaagac gtatcggcgg ccacgtttct
     4801 ccgccttgaa gtcatcgatg acacggatcc tttctttccg ccggcccagc ctgcgcccga
     4861 ccttcccgga tcggatcgga acccgcctgg cggccgggtg gcccgccgat ctcctctctt
     4921 cgcccgtcga ccccgcgctg cgccagctcc gacggcttga tatctgcaag cggcgcaagt
     4981 agctcccggt cgaacccggc cagcggctcg ccgccggcca accggttcgg ccagtccggg
     5041 ttggcgagcg cgccacggcc aagtgcaatg atgtccgccc catcctccag cagttgcaac
     5101 gcgcgctgcg gcgcgtgcag gctgccgttg gcgatcatgg tcacccccgg cgcgtacctg
     5161 cgggccagcg cgacaaggga agggccggtg tcgccgaagg ccgccttcca cgcttcgaat
     5221 tcggtgacat gcaggaaatc gagaccgacg tccgccagca agccaaacac gatccgcgcg
     5281 ccgtcttccc cttccggcca cttgtgctca aagtcgttga ccttggcctg tgagatgcgc
     5341 atgccgacgg gaaagtgcgt gccgaccgcc gcgcgtaccg cctgcgcaac ccccacgcaa
     5401 agacgcacgc gccgctcgat ggttccaccc cagtcgtcgc tgcggcggtt cgtatagccg
     5461 gtcaggaact ggtcgagcag atagccgttc gcgccgtgga tttccacccc gtcgaagccg
     5521 gccgcccgcg ccaggtttgc cgcacttgca aagccgtcga tcgccgcacg gatgtcggcg
     5581 ggggacatcg caagcggcgt gcgatacggt ccctggccgc cgtagaacgg catttgcagg
     5641 cccttggggg cgacagccga tggcccggct ggttcgccgc ggaagcggtt cccctgtgcg
     5701 agtgccccgg cgtgcatcag ctgggcgacg atctttcctc ccgccgcatg tacggcatcg
     5761 accaccgagc gccagccgcc cagctgctcg gcgtccgtca atcccggctg gttggcgtag
     5821 ccctgcgcat aggcgcgatc ggtgtagatg ccttcggtga tcaccagccc gaagccgccg
     5881 cgcgcaaagc gcgtgtagta gcgcaccatc gctggcgtgg gcacgccgag cggggtcgcg
     5941 ctcacgcgcg tcatcggcgc cacagcgagg cggttcgcca gttcgaggcc gttccgctcc
     6001 catttctcga acagcggccc gtccgcaagc tcggtgccgc tcattgctcg ccctccactg
     6061 ccagcgcgat cgcctctatc tcgatcatcg cgtccggcga gtaaagggcc gagatctcga
     6121 cgatcgtgtc ggccgggtag ggcttggcga accatttgcg gcgcatctcg acaatcgtgg
     6181 agaagtggtc catcgaggtc aggaagatcg tcaccttcac gacgcggtcc atgcccgagc
     6241 cgccggcctt gagcgcccgc tcgaggtttc ggaaagcctg gtcggcctga cgggcgaaat
     6301 ctcccttgcc gacgatgccg ccatcctcgc catacgccgc ctggccggac acgaacagca
     6361 gctggccggc gcggatggcc tgcgacagga ggtagggctc gtacgggtcg ggattggttt
     6421 tcacttgttc cagtttcatg gttctctcca ttcgatagtt gagttaggat caaccgcggt
     6481 caaacgcatt attggacgga tgagaggtag ggacaaatgg gaaaacatca gcatacggat
     6541 gagctgtact catccatgaa agtgcgatga gaaagcttcc gccgctcagc gcgctgcgcg
     6601 tgttcgaagc ggccgcgcgc cgcctgagtt tccggctagc cgccgaggaa ctgcatgtga
     6661 caccgacggc cgtcagccac cagatccgtc agctcgagga ctggatgggg gtccgcctgt
     6721 tcgagcgtgg cacgcgcaag gtgagcctga ccggtgccgg ggcccggctt tttccaccgc
     6781 tgcgcgacgg actcgacagt ttcgagcgcg cggtcgacga ggtgcggcga tcccaggctg
     6841 cgaaggtcgc cacgctgtcg tcgacggttg cattcatggc gcggcggctg gcgccgcttg
     6901 ccggttcctt tcgcagcgct tatccggact ggacgctgcg cctggacgcg tccaatgatc
     6961 cggtcgacct ggacggcgag gccgatgccg cgattcgcta cggtcgaggg cactatccgg
     7021 gcctggtggt ggttcccttg cttgcggacc gttttgctcc ggtatgcacg ccgcgcctgg
     7081 ccgcagcggc ccgcaaaggg ctgcgcggcg cgacgctgat ccacttcgag tgggggccga
     7141 tcgcgcgcgc ggatgaacag gcgcctacct ggcggcgctg gctggaggca gcgggaatga
     7201 cgggtgtgga cgccgaagcc gggttgtcgt ttaccgacga gatccatgcg gtccatgcca
     7261 cgctggccgg gcaggggatc ggcatgctga gcctgaacct ggtggcggac gagatcgcat
     7321 ccggcctgct ggtccagccg ttcgacgtgg ccatcggcaa ctatcgctac gatctcgtct
     7381 acagcccgcg cgccgcggcg cgcccggcga ccgtcgtgct ggtcgactgg gtcaggcgcg
     7441 cgttcggacc ggtgcgggaa acgggctgag ccgctacaga aagcggtcca gtatcttcag
     7501 gctcgcgcgg tccagcgcga gcatgtccgg caccaggaac tgaatgccgt ggctgtcgga
     7561 gatcagcagc atctgaggtc cgatgcggcg gatgtcttcc tcgccgcgca gtacgaaggc
     7621 cgccgcgccg cggtcggtca gcacatgcca ggtcgagggc gtcgcatagc tggtcacgcg
     7681 cgcgatcttc cggatgcgcg gcatgaactc ccgcacggcc aacgcttcct ccaccagttg
     7741 cctttgcgca ggcggcagtg catccagccg ctcgatccac gcggcttcgc ggccgtccgg
     7801 gcacacgagc gcgatgcctt cgtcgggcgc ctggatcgga aaggcgcgca ccggaaccac
     7861 ttcgttgaaa tgcgcgccgt cggcgcgcac gagctcaagg cggccgaagg cgttgcgtgc
     7921 aagcgcgaac ggcaaagctg cgtgcgatgt ggtgtcgttc atctcagtcc tcgctggccg
     7981 gcaccgccgc ttcggcgtgc gcgcgcgcct gggcctggta gagccggtag tacgcgcctt
     8041 cgcgcgccat cagttcgtcg tgcgcgcctt gctcaatcac ctggccgcgg tccagcacca
     8101 ccagccgatc ggcgcgctgc agcgtcgaga gccggtgcgc gatcgcaatc gtcgtgcggc
     8161 cgcgcaccag gttgtccagc gccttctgga tctccttttc cgtttccgaa tccaccgatg
     8221 cggtcgcttc gtccaggatc aggatgcgcg gatcgatcag cagtgcgcgt gcgatcgaga
     8281 tgcgctggcg ctcgccgccg gacaggccct ggccgcgttc gccgaccatc gagtcgtagc
     8341 cttgcggcag gcgcaggatg aattcgtgcg catgtgccgc gcgtgcggcg gcgacgatct
     8401 cggcacggct tgcatcaggc ttgccgtagg cgatgttctc ggcgatggtg ccgaagaaca
     8461 ggaagggctc ctgcagcacc aggccgatgt ggcgccggta gtcggcgatc gcaaatgagc
     8521 ggatgtcggt gccgtccacg cggatcgcgc cttcggccac gtcgtagaag cggcagatca
     8581 ggttgaccag cgtgcttttt cccgaaccgc tgtggccgac cagtccaagc atctcgccgg
     8641 gcgcgatgtc caggttgatg ccgcgcgcga cagagcggtt gccgtaccga aaagccacat
     8701 cgaccagctc gatgcgtccc ttgatctgcg cgcacggcac cggccgcgcc ggctccggca
     8761 cgctggagac gtggtcgagg atgtcgaaga tgcgcttggc ggctgacgcg gatttctgcg
     8821 tgaccgaaac gatgcggctc atcgcgtcga gccgtccata gaagcggctg atgtaggtga
     8881 cgaaggcggt cagcacgccg accgtgatgt cgccgtgcgc gacctgccag atgccgaacg
     8941 cccataccac caggagaccc agctccgtca gcaacgagac ggtcggagaa aacaccgacc
     9001 aggtcttgtt gagcctgtcg ttcactgcga ggttgtggcg gttcgtcgca cggaagcggt
     9061 cggcctcgcg cctttcctgt gcaaaggctt tcacgacgcg gatgccgggg atggtgtcgg
     9121 ccagcacatt ggtcacctcg ccccagacgc ggtcgatctt ctcgaagccg gtgcgcaggc
     9181 ggtcgcgcac cacgtggatc atccaggcga tgagcggcag cggcaacagc gtcaccagcg
     9241 ccagccacgg gttgatcgag aacaggatgg tggccgtcat caccagcatc agcacgtcgg
     9301 tcgcgaagtc gagcaggtgc agcgacagga acacgcagat gcgatccgtc tcgctgccga
     9361 tgcgcgcgat caggtcgccc gtgcgcttgc cgccgaagta ttcgtgcgac aggcgcagca
     9421 ggtgttcgta ggtggtggtg cgcagatctg cgccgatgcg ctccgacacc agggcgagga
     9481 tgtaggtccg ggcccatgag aggatccagg ccagcagcgc cgcgcccagc aggccgccca
     9541 tatacagcgc caccaatgaa gggtcgatct gcgtgccgtt ctggtacggg atcaggatct
     9601 cgtccatcag cggcatcgtc aggtagggcg gcaccaggct ggcggcggtg ccgagcaggg
     9661 tcaggatgaa cccggccagc aactggaggc ggtacggccg cgcgaagcgc cacagccgaa
     9721 acagggtcca ggtcgacggc ggcgtgtgga tgaccttggt gcagaccggg catgcatcct
     9781 ggtccggctc cagcggcgcc ttgcagcccg ggcaggcatt gcgctcgggt tcgcccgccg
     9841 gcttgccgcc gagccgcccg gtctgccgca gcacgaactg gtcgagcagg cgcatcgcat
     9901 gcaggttgtg gccgagcgtg aagtgccatg ccaccagccg gccgttcgcg tccaccagct
     9961 caaggtgtcc gacgccggca tgatcggcgt ggcgcagcgc cagtccttcc cggtagtccc
    10021 agtcggcgag ggtgctttca ccgggcgcct gggcgagcac gcgccggtcg gtcaccgcaa
    10081 gcagtccgct gccgaatgcg agccgtgcgt cgaggtcgat ctccatccat gcggcaatcg
    10141 attcgccggg ctgaagccgc ggatggacga tgcgttgcca gtggtcggga agatcgctgc
    10201 gggcgtcgat gccggccagt tcgatggggg gaggacgggt tgtcatgcgg atgtgcgagg
    10261 gtcggaccgg cgcggggcgg acgccgcgcg aggcggtagt gtgcgcggtc tggccaagga
    10321 tgtgaatcga aacttgccag agtgatcatt aatcttttgc gagacttaag ccaaacttag
    10381 acgtcgcacc caataattgc gcgtacatcg gccagcaggc gcgtattccc ctctcccatg
    10441 acgcacaaag acctccactt cctccgcgtg acccatctgc gcggtcccaa catctggacc
    10501 tatcgcccgg tgctcgaggc ctgggtcgat atcggcgcac tggaagatgc gccgtccaac
    10561 accatcgccg gactctatga gcggctgtcg gcgatgctgc cggggctcat cgagcatcgc
    10621 tgcggagtcg ggcgacgcgg cggcttcctg gaacggctga aggaaggcac ctgggccggc
    10681 catatcctcg aacatgtggt gctggaactg cagacgatgg ccggcatgaa gaccggtttc
    10741 ggcaaggcgc gcgagacctc ggtgcgcggt gtctacaagg tggtgttccg cacccggcac
    10801 gagcaggtcg ggcgcgcggc gctggatgcg gggcgcgcgc tgctgctggc ggcgatcgag
    10861 aaccggccgt atgacgtcgc cgcggccgtt gcgcgtctgc gcgggatggt cgacgatctc
    10921 tgccttgggc caagcaccgc gcacatcgtc gacgcggccg tcgagcgcgg cattccgttc
    10981 atccggctca ccgagggcaa cctcgtccaa ctcggccacg gcgtgcgcca gcgccgcatc
    11041 tggaccgccg agaccgacgc caccggcgcc atcgccgaag gcattgccgg cgacaaggat
    11101 ctgaccaagt cgctgctgcg agcctgcggc gtgccggttc ccgaaggggc gacggtcgat
    11161 agcgccgaag ccgcctggga agccgcgcag gagatcggcc tgccggtcgc gctcaagccg
    11221 caggacggca atcacgggcg cggggtgtcg ctggatctga tgtcgcgcga cgaagtgatc
    11281 gccgcgtatg aactcgcgcg cgcggaaggg gacggcggcg tgatcgtcga gcagttcatt
    11341 gccggcaatg aacatcggct gctcgtggt
//
LOCUS       DATLGF010000212         3441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_25237,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000212 DATLGF010000000
VERSION     DATLGF010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25237"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..978
                     /locus_tag="VM406_06415"
     CDS             202..978
                     /locus_tag="VM406_06415"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL75630.1"
                     /translation="MNPITALAREFSRSARARRAEIFRRTFDLNENTRILDLGSESGA
                     NIHAVLEGTPVRPENVYIADIEDHFLQEGRARYGYVPVLIGESGRLPFPDQFFDIVYC
                     SSVIEHVTVPKEQTWTLYSGKAFRSEALKRQKEFAAEIRRLGKQYYVQTPYRHFPIES
                     HSWLPFVGWLPRPVLIPVLHATNTFWVKKTEPDWYLLDKREMSELFPEARLVDEKSFG
                     FTKSMMAIGARRQPAATAGTRARRRTRRLRKAELGANLRN"
     gene            complement(1018..2271)
                     /locus_tag="VM406_06420"
     CDS             complement(1018..2271)
                     /locus_tag="VM406_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVL75631.1"
                     /translation="MSVLQKGAERRRVLILVENLPSPFDRRVWQEATTLAGNGYEVSI
                     ICPTGKGFEKKFEVINGIHIYRYNLPFEAKGAKGYAIEYSVALFWTFVLSLRVLFTRG
                     FDVVHACNPPDLLFLIGGFYKLVLRKCFLFDHHDINPELYEAKFGRRDFLYRMLRRLE
                     RWSFRVADVSIATNESYRKIAIERCGMPKEKVWVVRSGPKLDKWRIQPPNPSFKKGRR
                     FLVGYVGVMGQQEGIEFLLQAARHIVHDKKREDVQFVLVGGGPMLEDMRALSRTLQVD
                     DYVTFTGRVSDEELLEALNTADVCVNPDVANEMNDKSTMNKIMEYMALGKPIVQFDLV
                     EGRFSAREASIYVKRNDAIDMARGILELLADPERRAAMGAFGRRRVEHELEWRHEAPK
                     LLAAYDVLFGGAASKPGLAPARPHH"
     gene            complement(2308..>3441)
                     /locus_tag="VM406_06425"
     CDS             complement(2308..>3441)
                     /locus_tag="VM406_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HVL75632.1"
                     /translation="VLGRTLRATLDANEAVLGSDMSIICVGTPSARNGNLDLSHVRAV
                     CEQIGAALKDKDGFHLVVARSTMLPGSVREVVIPALERTSGKKAGAGFGVCINPEFLR
                     EGTAVYDYDHPPKTVIGEDDPEHGNMVASLYAHIDAPMIRTSFESAELVKYADNAWHA
                     LKVAFANEIGNLAKAIGTDGSEVMDYFCRDTKLNISSNYLKPGFAFGGSCLPKDVRAL
                     MYKARAMDLELPVLNAILPSNERQIEKGIRMIVDKQSRKVGILGFSFKAGTDDLRESP
                     MVDVIEHLIGKGYELKLYDQNVRIAALMGANQDYIVNRIAHISRLMANSIDEVLDFAG
                     TIVIGNNAPEFSELSRKVRPDQAVVDLVRALPSRSDSYEGICW"
BASE COUNT          623 a         1110 c         1083 g          625 t
ORIGIN      
        1 tgaccgcgct gttcccgggc agcctgatgc ggctgcgacc ggaatgggcg cccggcttgc
       61 ggccgcgctg agcgtggcca tcgcaacaag ggcatgattt gccggcgatc cggatttgag
      121 cgcgcgcaat aggagcgccg ccacactccg gacaatgagt gctcaccagc acttccagag
      181 cacgttcgcg aaggatagcc aatgaacccc atcaccgccc ttgcacgcga attttcccgc
      241 tcggcgagag cgcggcgtgc cgagatcttc cgccgcacat tcgacctgaa cgaaaacacg
      301 cgcatcctcg atctcggttc cgaatccggc gccaatatcc acgcggtcct ggaaggaacg
      361 ccggtcaggc ccgagaacgt ctatatcgcc gacatcgaag atcatttcct gcaggagggc
      421 agggcgcgat acggctatgt gccggtgctg atcggcgagt cggggcggct gccgtttccg
      481 gaccagttct tcgacatcgt ctactgctcc tcggtgatcg agcacgtgac ggtgccgaag
      541 gagcagacat ggacgctcta ttccggcaag gcgttccgca gtgaagcgct gaagaggcag
      601 aaggaattcg cagccgagat ccgccggctg ggcaagcagt attacgtgca gacgccgtac
      661 cgccacttcc cgatcgagag ccacagctgg ctgcctttcg tgggctggct gccgcgcccg
      721 gtgctgattc ccgtgctgca tgcgacgaac acgttctggg tcaagaagac cgagccggac
      781 tggtacctgc tggacaagag ggagatgagc gagctgtttc cggaagcgcg gctggtcgac
      841 gagaaatcct tcggattcac gaaatcgatg atggccatcg gcgccaggcg tcaaccggcc
      901 gccacggcag gcacgcgcgc gcgccgccgc acccgtcgcc tgcgcaaagc ggaactcggc
      961 gcaaacctgc gcaactgacc cgcgcgggaa ggcccggcgc cccgccgggg ccgcgcgtca
     1021 gtgatgcggg cgcgccggcg cgagtccggg cttgctcgcc gcgccgccga acagcacgtc
     1081 gtacgccgcc aggagtttcg gcgcctcgtg gcgccattcg agctcatgtt cgacgcggcg
     1141 ccgtccaaac gcgcccatgg ccgcgcgccg ttcggggtcg gccagcagct cgaggatgcc
     1201 gcgtgccatg tcgatcgcgt cgttgcgctt cacgtagatc gacgcctcgc gtgccgagaa
     1261 gcggccctcg acgagatcga actgcacgat cggcttgccc agcgccatgt attccatgat
     1321 cttgttcatc gtcgacttgt cgttcatctc gttggccaca tccggattca cgcacacgtc
     1381 cgcggtattg agcgcctcga gcagttcctc gtccgagacg cggccggtga aggtcacgta
     1441 gtcgtccacc tgcagcgtgc gggacagcgc gcgcatgtcc tccagcatcg gcccgccgcc
     1501 gacgagcacg aactgcacgt cctcgcgttt cttgtcgtgc acgatgtggc gtgcggcctg
     1561 cagcaggaac tcgatgcctt cctgctgccc catcacgccg acgtagccga ccagaaagcg
     1621 ccgtcccttc ttgaatgagg gattgggcgg ctggatgcgc cacttgtcga gcttgggccc
     1681 gctgcgcacg acccacacct tctccttcgg catgccgcag cgttcgattg cgatcttgcg
     1741 atacgactcg ttggtcgcga tcgacacgtc ggcgaccctg aacgaccagc gctcgagccg
     1801 gcgcagcatc cggtagagga aatcgcggcg gccgaacttc gcctcataca gttccggatt
     1861 gatgtcgtga tgatcgaaca ggaagcactt gcgcagcacc agcttgtaaa agccgccgat
     1921 caggaacagc aggtcgggcg gattgcaggc gtgcacgacg tcgaagccgc gcgtgaagag
     1981 cacccgcagt gacagcacga aggtccagaa cagggcgacg ctgtactcga tcgcgtagcc
     2041 ctttgcaccc ttggcttcga aaggcaggtt gtagcggtag atgtggatgc cgttgatgac
     2101 ttcgaacttc ttttcgaaac ccttgccggt cgggcagatg atcgaaactt catacccatt
     2161 gcctgccagg gtggtcgcct cttgccagac gcgccggtcg aacggggatg gaaggttctc
     2221 gaccaggatc agtacgcgtc gtcgctcggc gcctttttgg agcacactca tgtttgcctc
     2281 gttacgcaac aggaaagcgg acagcggtta ccagcagatg ccctcgtagg agtcggagcg
     2341 cgagggaagc gccctgacaa ggtcgaccac cgcctggtcg ggccgcacct tgcggctcag
     2401 ttcggaaaac tcgggcgcat tgttgccgat gacgatcgtg cccgcgaaat cgaggacctc
     2461 gtcgattgaa ttggccatca ggcgggaaat gtgcgcaatg cggttgacga tgtagtcctg
     2521 gttcgcgccc atcagcgccg cgatgcgcac gttctggtcg tacagtttga gctcgtagcc
     2581 cttgccgatc agatgctcga tcacatccac catgggcgac tcgcgcaggt cgtccgttcc
     2641 tgccttgaac gagaacccga ggatgccgac cttgcggctc tgcttgtcga cgatcatgcg
     2701 gatgcccttc tcgatctggc gctcattgga ggggaggatg gcgttaagca ccggcagctc
     2761 gaggtccatc gcacgcgcct tgtacatgag cgcgcgcaca tccttcggca ggcaggagcc
     2821 gccgaacgcg aacccgggct tcagatagtt ggacgagatg ttgagcttgg tatcgcggca
     2881 gaaatagtcc atcacctcgg agccatccgt gccgatcgcc tttgccagat tgccgatctc
     2941 gttggcgaac gccaccttca gcgcatgcca ggcattgtcg gcgtacttca ccagctcggc
     3001 cgactcgaag ctcgtgcgaa tcatcggggc atcgatatgc gcatagagcg atgcgaccat
     3061 gttgccgtgc tccggatcat cctcgccgat cacggtcttc ggcgggtggt cgtagtcata
     3121 cacggccgtg ccttcgcgca ggaactccgg gttgatgcac acgccgaatc cggcgccggc
     3181 cttctttccc gaggtgcgct cgagcgcggg gatcacaacc tcgcgcacgg agcctggcag
     3241 catcgtgctg cgcgcgacga ccaggtggaa gccgtccttg tccttcaagg ccgcaccgat
     3301 ctgctcgcag acggcccgca cgtgcgacag gtccaggttg ccgttgcgcg ccgacggcgt
     3361 cccgacgcag atgatcgaca tgtccgaccc gagcaccgcc tcgttcgcgt ccagggtggc
     3421 gcgcagcgtc cgccccaaga c
//
LOCUS       DATLGF010000213         5297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_115980, whole genome shotgun sequence.
ACCESSION   DATLGF010000213 DATLGF010000000
VERSION     DATLGF010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115980"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VM406_06430"
     CDS             complement(<1..206)
                     /locus_tag="VM406_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVL75633.1"
                     /translation="MELQFLGTSSGTPTKTRNVSALAIRATGSRQWSLVDCGEGTQHR
                     ILQTRLSLRSLHAIFITHTHGDHC"
     gene            523..1002
                     /locus_tag="VM406_06435"
     CDS             523..1002
                     /locus_tag="VM406_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL75634.1"
                     /translation="MEIQEYRPGALGRMVELHAAYYSEAAGFGAFFEAKVASEMAAFL
                     QDYRPGMDGMWTMAADGAIEGGVAIQLTDRQQGHADLRWFILSERVRGKGMGSALLER
                     ALRHCRDCGMSRVTLWTFAGLHAARTLYERQGFRLVEERSGRQWGSEVLEQRFVLDL"
     gene            complement(1137..2954)
                     /gene="glmS"
                     /locus_tag="VM406_06440"
     CDS             complement(1137..2954)
                     /gene="glmS"
                     /locus_tag="VM406_06440"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HVL75635.1"
                     /translation="MCGIVGAVAQRNITPILIEGLKRLEYRGYDSCGVALHVDGTLQR
                     SRSTSRVADLEAQVDKAGLAGFTGIAHTRWATHGAPASHNAHPHFSRERIALVHNGII
                     ENHDELRAELSAAGYQFESQTDTEVIAHLVDHMYTGDLFETVRQAVKRLHGAYAIAVF
                     SRDEPHRVVGARQGSPLIVGVGKGENFLASDAMALAGTTDQIIYLEEGDVVDLQLQRY
                     WIVDGDDRPAEREVRTVHAHTGAAELGPYRHYMQKEIFEQPRAIGDTLEGVTAIMPEL
                     FGDGAYKVFKQVDSVLILACGTSYYAGLTAKYWIESIAKLPVNVEIASEYRYRDSVPN
                     PNALVVTISQSGETADTLAALKHARKQDMVHTLTICNVATSAMVRECAMSYITRAGVE
                     VGVASTKAFTTQLVALFLLTLALAQVRGRLTEEEEANCLKQLRHLPAAIGAVLALEPQ
                     IIAWAEEFARRENALFLGRGLHYPIALEGALKLKEISYIHAEAYPAGELKHGPLALVT
                     NEMPVVTVAPNDALLEKLKSNMQEVRARGGQLYVFADADSHISSSEGVHVIRMPEHYG
                     KLSPILHVVPLQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            3108..3578
                     /locus_tag="VM406_06445"
     CDS             3108..3578
                     /locus_tag="VM406_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HVL75636.1"
                     /translation="MNDPAALDELDRRILNALQQDASRTNNELAELVHASAPTCLRRV
                     KRLVELGVIARQVALVAPEKLGPALTAIVEITLDHQGAERVVEFEALVASEPEVLQCY
                     RVSPGPDFVLIVQVADMPAYHAFAHRVFAAHANVRNVRSFFSIHRSKFETRIAL"
     gene            complement(3686..5044)
                     /gene="glmU"
                     /locus_tag="VM406_06450"
     CDS             complement(3686..5044)
                     /gene="glmU"
                     /locus_tag="VM406_06450"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HVL75637.1"
                     /translation="MNVVILAAGMGKRMQSSLPKVLHPLAGKPLLSHVIDTARTLSPS
                     RLCVIYGHGGEAVPQAISADDIVFAKQEPQLGTGHAVMQAVPHLNDDVPTLVLYGDVP
                     LTTGNSLRQLLDSAGPDKLAILTIELTDPTGYGRILRENGAIVGIVEQKDATPQQRAI
                     REVNTGILVAPTAWLKQALNSLSNDNAQGEYYLTDVVARAVADGVPVVSAQPGADWET
                     LGVNSKVQLAELERIHQRNIANSLLEAGVTLADPARLDVRGSLSCACDVVIDVNCVFE
                     GTVVLEEGARIGPNCFIRNARIGAGARIQPFCHIEEAQVGAASVIGPYARLRPGTELG
                     EDVHIGNFVEVKNSQIAAHSKANHLAYVGDATVGARVNIGAGTITCNYDGANKFRTVI
                     EDDAFIGSDTQLVAPVRVGKGATLGAGTTLTKDAPEGKLTVSRAKQVTIDGWARPVKT
                     KK"
     gene            5063..>5297
                     /locus_tag="VM406_06455"
     CDS             5063..>5297
                     /locus_tag="VM406_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75638.1"
                     /translation="MQNSSMGPRRTFLSRIIAAALAAVLVIALAGCSALRLGYANGET
                     FVYWWMNSYVGFNDEQKPWVKEEIARFFAWHRQT"
BASE COUNT          895 a         1672 c         1748 g          982 t
ORIGIN      
        1 tagcagtggt cgccgtgcgt gtgcgtgatg aaaatcgcat gcaggctgcg cagcgacagc
       61 ctggtctgca gaatcctgtg ctgcgttcct tctccgcaat cgaccaggct ccactgccgc
      121 gatccggtag cgcggatcgc cagcgccgac acgttcctgg tttttgtggg cgtgccggac
      181 gaggtgccga ggaactgcag ttccattttt tctccggttc gttcgagacc cgccgcccgc
      241 catgtgcggc ttgcggggca tgacaggcgc gcaagagcga ccgcccgagt cattcgatcc
      301 gttcggacgc gcgcaggttt cgggcgtcgg ccgggtggag cggcgcacaa ctgcggctgc
      361 cggctgcatg caccatccgc attgcaccgg ggcggaaatg ttgcttgaac tgactacgtg
      421 cctggcctaa gttgcttgtc gacgcagtag tacgagggca ccgccggggt ccgggaaaaa
      481 gcggttggca gggctgcgcg caccgggaca agcgagagaa gcatggaaat tcaggaatac
      541 cgtccggggg cgctcggacg catggtggaa ttgcacgcag cctactacag cgaggcggcc
      601 ggcttcgggg catttttcga agccaaggtc gcaagcgaga tggccgcatt tcttcaggac
      661 tatcgtcccg gcatggacgg catgtggacc atggcggccg atggcgccat cgaaggcggg
      721 gtggcgatcc agttgacgga ccggcagcaa ggacatgccg atctgcgctg gttcatcctg
      781 tcggagcggg taagaggcaa gggaatggga tcggcgctgc tcgagcgtgc attgcggcat
      841 tgccgcgact gcggtatgag ccgggtcact ctgtggacat tcgccggcct gcatgcggcg
      901 aggacgctct acgagcggca gggatttcgt ctggtcgaag agcgcagtgg acggcagtgg
      961 gggagcgagg tgcttgagca gcgcttcgtg ctcgacctct agcgggatcg catcgacgcg
     1021 cgccggactg cggccgcggc agcgcgtccg cgatgacgac ccggaggtgt tcacgcgctc
     1081 cctgtgcagg ccgccggccc gttgcagcca cgtcgataga acgttggggg cgaggattac
     1141 tcgaccgtga ccgacttcgc cagattacgc ggcttgtcca catcggtgcc gcgcgccagc
     1201 gcggtgtgat atgcgagcag ctgcagcggc accacgtgca ggatgggcga gagcttgccg
     1261 tagtgctcgg gcatgcggat cacgtgtacg ccctcgctcg aactgatgtg cgaatcggcg
     1321 tcggcgaata cgtaaagctg gccgccgcgc gcgcgcactt cctgcatgtt cgacttcagt
     1381 ttctccagca gcgcgtcgtt cggcgcaacc gtcacgaccg gcatctcgtt cgtcaccagc
     1441 gccagcgggc cgtgcttcag ttcgccggcc ggataggctt cggcgtggat gtaggagatt
     1501 tccttgagct tcaatgcgcc ttccagcgcg atcggatagt gcaggccgcg cccgaggaac
     1561 agcgcgttct cgcgccgcgc aaattcctcc gcccaggcga tgatctgcgg ctccagcgcc
     1621 aacaccgcgc cgatcgcggc cggaagatgg cgcagctgct tcaggcagtt cgcttcttct
     1681 tcctcggtca ggcgcccgcg cacctgggcc agcgccagcg tcagcaggaa cagcgcgacc
     1741 agctgggtcg tgaacgcctt cgtcgatgcg acgccgacct ccaccccagc gcgggtgatg
     1801 taggacatcg cgcattcgcg caccatcgcg ctggtcgcaa cgttgcagat cgtcagcgta
     1861 tgcaccatgt cctgcttgcg cgcatgcttg agtgccgcca gcgtgtcggc cgtctcgccg
     1921 ctttgcgaaa tcgtcaccac cagcgcgttc gggttcggca cgctgtcgcg gtagcggtac
     1981 tcgctggcga tttccacatt caccggcagc ttggcgatcg attcgatcca gtacttcgcg
     2041 gtcaagcccg cgtaatagct ggtgccgcag gcgaggatca ggaccgaatc gacctgcttg
     2101 aacaccttgt aggcgccgtc gccgaacagc tcgggcatga tcgcggtcac gccttcgagc
     2161 gtgtcgccga tcgcgcgcgg ctgctcgaag atttccttct gcatgtaatg gcggtacggc
     2221 cccagctcgg cggcgccggt gtgcgcgtgc acggtgcgca cctcgcgctc ggccgggcgg
     2281 tcgtcgccgt cgacgatcca gtagcgctgc agctgcaggt cgaccacgtc gccttcttcc
     2341 aggtagatga tctggtcggt cgtgccggcc agcgccatcg cgtcggacgc gaggaagttc
     2401 tcgcccttgc cgacgccgac gatcagcggc gaaccttggc gcgcgccgac cacgcggtgc
     2461 ggctcgtcgc gcgagaacac cgcgatcgca tacgcgccgt gcaggcgctt gaccgcctgg
     2521 cgcacggtct cgaacagatc gcccgtgtac atgtggtcga ccaggtgggc gatcacctcg
     2581 gtatcggtct ggctctcgaa ctggtagccg gcggcgctca gttccgcgcg caattcgtca
     2641 tggttctcga tgatgccgtt gtgaaccagc gcgatgcgct cgcgcgagaa gtgcggatgg
     2701 gcattgtgcg acgcgggcgc gccgtgggtg gcccagcggg tgtgggcgat gccggtgaag
     2761 ccggccaggc ccgccttgtc cacctgcgcc tccaggtcgg ccacgcgcga ggtgctgcgc
     2821 gaacgctgca gcgtgccgtc cacgtgcagc gcaacgccgc aggagtcata gccgcggtat
     2881 tcgagccgct tcaggccctc gatcaggatg ggggtaatgt tccgctgcgc aaccgcgccg
     2941 actatgccgc acatgtgagc ctcgctgaaa agtgaatgct gtggatgcta gaagaggcga
     3001 ggagaaaaat gcttgcgaaa gcgcacggaa aatgaccgaa tatttcgcga catctgtaac
     3061 aggaaatata atttcacttt tcggatgtaa gcgaatagga atttcatatg aatgatcccg
     3121 ccgcgctgga tgagctggac cgtcgcatcc tcaatgccct gcagcaggat gcctcgcgca
     3181 ccaacaacga gttggccgaa ctcgtccatg cgtcggcccc cacctgcctg cgccgcgtga
     3241 aacggctggt ggaactcggc gtgattgcac gccaggtggc gctggtggcg ccggagaagc
     3301 ttgggccggc gctgaccgcg atcgtcgaaa tcacgctcga ccatcaggga gcggagcgcg
     3361 tcgttgaatt cgaggcgctg gtcgccagcg agccggaagt gctgcagtgc taccgggttt
     3421 cgccgggtcc tgatttcgta ttgatcgtgc aagtggcgga catgcctgcc taccacgcgt
     3481 tcgcgcaccg cgtgttcgcc gcgcatgcga acgtgcgcaa tgtgcgcagc ttcttctcga
     3541 tccaccgcag caagttcgaa acgcgtattg cgctttgacg tccggagaaa agccaaagga
     3601 acgagccacg gcgaagacgg ggaatacaga agaaacaatc gcggctttca ccgtgcccgc
     3661 cgtggtgaaa ggaactggtg ccgcgctatt tcttggtctt caccggccgc gcccacccgt
     3721 cgatcgtcac ctgcttcgcg cgcgagaccg tcagcttgcc ttccggcgcg tccttggtca
     3781 gcgtggtgcc ggcgccgagc gtcgcgccct tgccgacgcg cactggcgcg acgagctggg
     3841 tatcgctgcc gatgaaggca tcgtcctcga tcacggtgcg gaacttgttc gcgccgtcgt
     3901 agttgcaggt gatcgtgccg gcgccgatgt tcacgcgcgc gccgacggtc gcatccccca
     3961 cataggcgag gtggttggcc ttgctgtgcg cggcgatctg gctgttcttc acttcgacga
     4021 agttgccgat gtggacatcc tcgcccagct cggtgccggg ccgcagccgc gcatacggac
     4081 cgatcaccga ggccgcgccg acctgcgctt cctcgatatg gcagaacggc tggatgcgcg
     4141 cgccggcacc gatgcgcgca ttgcggatga agcagttggg accgatgcgc gcgccttcct
     4201 ccagcactac cgtgccttcg aacacgcagt tcacatcgat gacgacatcg catgcgcagg
     4261 acaggctgcc gcgcacgtcc aggcgcgccg ggtcggccag cgtcacgccc gcctcgagca
     4321 gactgttcgc aatattgcgt tgatgaatgc gctccagctc cgccagctgc accttgctgt
     4381 tgacgcccag cgtctcccag tccgcgcccg gctgcgccga gacgaccgga accccatcgg
     4441 ccaccgcgcg cgcgaccacg tcggtcaggt agtactcgcc ctgggcattg tcgttcgaca
     4501 ggctgttcag tgcctgcttg agccaggctg tcggtgcgac caggatgccg gtattgactt
     4561 cgcgaatcgc ccgctgctgc ggcgtcgcat ccttctgctc gacgatgccg acgatcgcgc
     4621 cgttctcgcg caggatgcgg ccataacctg tgggatcggt caattcgatg gtgagtatcg
     4681 ccaacttatc gggcccggca ctgtccaaga gttgccgcaa cgaattgcct gttgtcagtg
     4741 gcacatcgcc atacagcacc agcgttggca cgtcgtcgtt caggtgcggc accgcctgca
     4801 tgacggcatg gccggttccg agctgcggtt cctgtttggc aaagacgatg tcgtcagcgg
     4861 aaatggcttg cggcacggct tcgccgccgt ggccgtagat cacgcatagt cgcgatggcg
     4921 acagcgtgcg ggcagtatcg atgacgtggg agagcagcgg cttgcccgcc agcggatgca
     4981 acaccttggg aagcgaggac tgcatgcgct tgcccatgcc ggcagcaaga atgacaacgt
     5041 tcataacgta cgattgaaag cgatgcaaaa ttctagcatg ggcccccggc gcaccttttt
     5101 gtcgcgcatc atcgccgcgg cactcgctgc cgtgctcgtc atcgcgctgg ccggctgcag
     5161 cgcgctgcgc ctcggctatg cgaacggcga aaccttcgtc tactggtgga tgaacagcta
     5221 cgtcggcttc aatgacgagc agaagccctg ggtgaaggag gagatcgccc ggttcttcgc
     5281 ctggcaccgc cagaccc
//
LOCUS       DATLGF010000214         5130 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_87674,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000214 DATLGF010000000
VERSION     DATLGF010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87674"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..995)
                     /locus_tag="VM406_06460"
     CDS             complement(21..995)
                     /locus_tag="VM406_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL75639.1"
                     /translation="METQYITLEQWRTLVTVVERGGYAQAAEALHKSQSSVTYAIQKM
                     ESQLGVQAFEIQGRKAVLTPTGQFLYRRAKSLMEESLALERAARRVSAGWEPEIRIAA
                     EIIFPTWLLLDCLARFGQESPHTRIELIESVLSGTTEALLTGQADIAIASTVPPGFAG
                     TPLLQPRLILVASPDHPLHGLGRPVTVRDLNAHRHIVIRESGSRRDSGMLLVETAQRW
                     TVSNAATAIAALKRGYGFAWFAEEKIRDELRDGTLTPLPMNEGGERYGQLSLVLADPD
                     AAGPGTRRLVELIREALAHECLRYHAPGEGVRGQGAAAIRMIGASSEP"
     gene            1095..1715
                     /locus_tag="VM406_06465"
     CDS             1095..1715
                     /locus_tag="VM406_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HVL75640.1"
                     /translation="MKTLLHINSSLFSGAGQSSQLAQQFVAGWKSAHPDGEVIIRDIG
                     AEPVPHLDAERFTALISKAEERTPAQQEIVAYADALLDEVRRADIIVLGLPMYNFGVP
                     SSLKAYFDHLARAGVTFRYTENGAVGLLGGKKVYVFATRGGAYQGTPADSQTPFVRTF
                     LSFIGLDDIEFVYAERLNMGEDSKQSALRDAQHAIGQLVEPLRDAA"
     gene            1734..2609
                     /locus_tag="VM406_06470"
     CDS             1734..2609
                     /locus_tag="VM406_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HVL75641.1"
                     /translation="MTTASDTSRTLQRVIASMATSDGAGVKLRRSLGQRQGMRLDPFL
                     MLDEFGTDNPGDYIAGFPPHPHRGFETVTYMLDGHMRHEDHLGNVGELTAGGVQWMTA
                     GRGIIHSEMPQQKEGRMRGFQLWINLPAAEKMKPAGYRDIPATDIPVAELPGGVRVKV
                     IAGSVDADGKAVAGPIQGLSTEPVYLDVALEAGARFAYPLPQGHNAFVYTYEGSVAIG
                     PDARLQPPQSAGILSPGELVEVVAHDVPARFLLLAGKPLGEPVVQYGPFVMNSVEEIQ
                     QAIADYQSGTFAQPA"
     gene            2684..3598
                     /locus_tag="VM406_06475"
     CDS             2684..3598
                     /locus_tag="VM406_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HVL75642.1"
                     /translation="MPHTAFHLIKPHVKELGDGFTVRRSLPSHPHKMVGPFIFFDHMG
                     PAHLEPGGLGLQVRPHPHIGLATVTYLFEGAIMHRDSLGYQQEIRPGDVNWMTAGSGI
                     VHSERTPEHLRAEGGTMHGIQIWIALPKSDEFTEPHFEHHPGATLPEIRGEGVHLRLI
                     AGAGFGLTSPVTTFSPLFYAALDLAPGARFTLPAEHPERAVYAVEGEVLVDGEVLPET
                     AMAVLSAGQDTTIEAPAQTGTGARLMLLGGEPLDGDRYISWNFVASSREAIEEARQRW
                     RERAFGKVPGETEWIPLPDERKAPESFS"
     gene            3645..4295
                     /gene="ribA"
                     /locus_tag="VM406_06480"
     CDS             3645..4295
                     /gene="ribA"
                     /locus_tag="VM406_06480"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HVL75643.1"
                     /translation="MSTPGDQNAAVAGSDDLFDHVATSLLPTRHGEFRIHVFRPVDET
                     VEHVAIIRGEPRDREGVLVRVHSECLTGDVLGSLRCDCGEQLDAALARIAEAGLGAVL
                     YLRGHEGRGIGLANKIRAYQLQDQGSDTVEANLALGLPVDTRDYQVAARILSYLGVKS
                     IRLMTNNPRKMNKLAEYGVDIVERVPVITAHTPHNVGYLRTKQQKLGHFLDFEAEG"
     gene            complement(4504..>5130)
                     /locus_tag="VM406_06485"
     CDS             complement(4504..>5130)
                     /locus_tag="VM406_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HVL75644.1"
                     /translation="APASPLDAPARPQRHSALDDVQALLTGTLLVSLGVTLLGEARLV
                     TGGTVGIAFLLHYLSGIGFGKLFFAVNLPFAWLALRKMGWRFTLKTFAAIGLLSLLSD
                     MLPRLIDLERLDPLYAAVMGGLLCGVGLLALFRHSASLGGLNVLVLWLQERFGWRAGY
                     VQLALDAAILLMSIPVISPAAVLISLVGAVALNLTLAINHKPGRYMAI"
BASE COUNT          926 a         1710 c         1670 g          824 t
ORIGIN      
        1 gctttcctgc tcaagcagga tcagggctcc gaagacgcgc cgatcatgcg gattgcagca
       61 gcgccttgcc cccgcacacc ctctcccgga gcgtgatagc gcaggcattc gtgcgccagc
      121 gcctcgcgga tcagctcgac cagccgccgc gtgccggggc cggccgcatc gggatcggcg
      181 agcaccagcg acagctggcc gtaacgctcg cctccctcgt tcatcggcaa cggtgtgagc
      241 gtgccgtcgc gcagttcgtc gcggattttt tcttcggcga accaggcgaa gccgtagccg
      301 cgcttgagcg cggcgatcgc ggtcgccgca ttgctgacgg tccagcgctg cgcggtttcg
      361 accagcagca tgcccgagtc gcgccggctt cccgactcgc gaatcacgat atgccgatgc
      421 gcgttcaggt cgcgcacggt gaccggacgg ccaagcccgt gcagcggatg gtcggggctg
      481 gcgacaagta tcaggcgtgg ctgcagcaac ggcgtgccgg cgaagccggg tggcaccgtg
      541 ctggcgatgg cgatatccgc ctgtccggtg agtagtgcct cggtggtgcc gctgagcacc
      601 gattcaatca gctcgatgcg cgtgtgcggg ctttcctgcc cgaagcgggc gaggcagtca
      661 agcagcagcc aggttggaaa aatgatctcg gcggctatcc ggatttccgg ctcccagccg
      721 gcagagaccc gccgcgccgc gcgttccagc gccagcgatt cctccatcag cgacttcgcg
      781 cgccggtaca gaaactggcc ggtcggcgtc agcacggcct tgcgtccctg tatttcgaat
      841 gcctgtacgc ccagttgcga ctccattttc tggatcgcgt aggtcaccga cgactggctc
      901 ttgtgcaagg cttcggccgc ctgcgcgtag ccgccgcgtt cgaccacggt caccagcgtg
      961 cgccattgtt cgagcgtgat gtattgggtt tccatatcta ttaattcgat gaattagacc
     1021 gaatcattct acttttcaat cggcccaatg gatagtttaa tgcagggaca gacaaacctc
     1081 tttggagaac gaatatgaag acccttctgc acatcaactc ctccctgttt tccggtgccg
     1141 gccagtcgag ccagctcgcc cagcagttcg tcgcaggatg gaagtccgcg catccggacg
     1201 gcgaagtcat catccgcgac atcggcgccg agccggttcc gcacctcgac gccgagcgtt
     1261 tcacggccct gatctcgaag gccgaggagc gcacgcccgc ccagcaggag atcgtcgcct
     1321 atgccgatgc gctgctcgat gaagtgcgcc gggccgacat catcgtgctc ggcctgccga
     1381 tgtacaactt cggcgtgccg tcttcgctga aggcctactt cgaccacttg gcgcgcgccg
     1441 gcgtgacctt ccgctatacc gagaacggcg cggtcggcct gctgggcggc aagaaggtct
     1501 atgtgttcgc cacgcgtggc ggcgcctacc agggaacgcc ggccgacagc cagacgcctt
     1561 tcgtgcgcac gtttttgtcc ttcatcggtc tcgatgacat cgagttcgtg tacgcggagc
     1621 gtctgaatat gggtgaagat agcaagcaga gcgcactgcg tgacgcccag cacgcaatcg
     1681 gccagctggt cgagccgctg cgcgacgcgg cctgacacga aaaggagatc gaagtgacca
     1741 cggcatccga cacctcccgc acgctgcagc gtgtgatcgc cagcatggcg acctccgacg
     1801 gcgccggcgt gaaactgcgc cgcagcctgg ggcagcgcca gggcatgcgg ctggatccct
     1861 ttctgatgct ggacgagttc ggcaccgaca accccggcga ctacatcgcc ggattcccgc
     1921 cgcacccgca ccggggtttc gaaaccgtca cctacatgct cgacggccac atgcgccacg
     1981 aggatcacct cggcaacgtc ggcgagctga ccgcgggcgg cgtgcagtgg atgacggcag
     2041 gccgcggcat catccattcc gagatgccgc agcagaagga gggccgcatg cgcggcttcc
     2101 agctgtggat caacctcccg gcggcggaga agatgaaacc ggccggctac cgcgatatcc
     2161 cggcgaccga cattccggtc gccgagctgc cgggcggcgt gcgcgtgaag gtgatcgccg
     2221 gcagcgtcga cgccgacggc aaggcggtag ccgggccgat ccagggcctt tcgaccgagc
     2281 cggtctacct cgacgtggcg ctggaggcgg gcgcgcgctt tgcgtatccg ctgccgcagg
     2341 gtcacaatgc cttcgtctat acgtatgaag ggagcgtggc gatcggtccc gacgcacggt
     2401 tgcagccgcc gcagagcgcc ggcatcctgt cgccgggcga actggtcgag gtcgtcgccc
     2461 acgacgttcc ggcgcgcttc ctgctgctgg ccggcaagcc gctgggagag ccggtcgtgc
     2521 aatacgggcc attcgtgatg aacagcgtgg aagagatcca gcaggcgatc gcggattacc
     2581 agagcgggac tttcgcgcag cctgcttgag cgctggcggt gcggccgaag cgccggcggg
     2641 cgacgatacg atttttgcag cgcttgacga acaggagacc tccatgccgc acaccgcttt
     2701 ccacctgatc aagccccatg tgaaggagct cggcgacgga ttcaccgtgc gccgctcgct
     2761 tcccagccat ccgcacaaga tggtcgggcc tttcatcttc ttcgaccaca tggggcccgc
     2821 ccatctcgag cccggcgggc tgggcctgca ggtgcggccc catccgcata tcgggctggc
     2881 gaccgtcacc tacctgttcg aaggcgcgat catgcaccgc gattcgctcg gctaccagca
     2941 ggaaatccgg cccggcgatg tgaactggat gacggccggc tccggcatcg tccattccga
     3001 gcgcacgccc gagcacctgc gcgcagaggg cggcacgatg catggcatcc agatctggat
     3061 cgcattgccg aaatcggatg agttcaccga gccgcatttc gaacatcacc cgggcgcaac
     3121 gctgccggag atccggggcg agggcgtcca cctgcgcctg atcgccggcg caggattcgg
     3181 cctgacatcc ccggtgacca cgttctcccc gctgttctac gccgccctcg atctggcgcc
     3241 cggcgcgcgc ttcacgctcc ccgccgagca tccggagcgg gccgtgtacg cagtcgaagg
     3301 cgaagtcctc gtggacgggg aagtcctgcc cgaaacggca atggccgtgc tgtccgccgg
     3361 gcaagacact acaatagagg ccccagcgca aaccggaacg ggcgcgcgcc tgatgctgct
     3421 gggcggcgaa ccgctcgacg gcgaccgcta catctcctgg aatttcgtgg catccagccg
     3481 cgaggcgatc gaggaggcgc ggcaacgctg gcgcgagcgg gccttcggca aggtgccggg
     3541 agaaaccgag tggatcccgc tgcccgacga acgcaaggcg cccgagtctt tcagctaaac
     3601 cgcatgtcat gccggaatga caggcatgac caggacacca gaacatgagc acccccggag
     3661 accagaacgc ggcagttgcc ggctccgacg accttttcga tcacgtcgcc acctcgctgc
     3721 tgccgacgcg gcatggcgag ttccgcatcc atgtattccg gccggtcgac gagacggtcg
     3781 agcatgtggc catcatcagg ggcgagccgc gcgaccggga aggggtgctg gtgcgcgtgc
     3841 attcggaatg cctgaccggc gacgtgctcg ggtcgctgcg ctgcgactgc ggcgaacagc
     3901 tcgatgcggc gctggcccgc atcgccgaag ccggcctcgg agcggtgctg tacctgcgcg
     3961 gccacgaggg gcgcgggatc ggcctggcga acaagatccg cgcgtaccag ctgcaggacc
     4021 agggcagcga caccgtggaa gcgaatctcg cgctcggcct gccggtggat acgcgcgact
     4081 accaggtcgc cgcgcgcatc ctgagctatc tgggtgtgaa gtcgatccgc ctgatgacga
     4141 acaatccgcg caagatgaac aagctcgccg agtatggggt ggatatcgtc gagcgcgtgc
     4201 cggtcattac cgcccatacg ccgcacaacg tcggctacct gcgcacgaaa cagcagaagc
     4261 tcgggcattt cctggatttc gaagcagaag gctgagcgcc ggggctggtc cgtcattccg
     4321 caccgccgcc caacaacctg tcatcctgag ccgaaggcga aggatctcat cgctcgataa
     4381 cggtggtgtc gaccggcaag aggatgaggt ccttcgccgg tggctcagga cgacaagctt
     4441 tcccgcggac ttgtcatcct gcgccgaagg cgaaggatct catccgcctc gtattgccac
     4501 atgctagatc gccatgtacc ggccgggctt gtggttgatc gccagcgtca ggttcagcgc
     4561 caccgcgccc accagcgata tcagcaccgc cgcaggcgag atcacgggaa tcgacatcag
     4621 caggatcgcc gcatccagcg ccagctgcac atagccggca cgccagccga agcgttcctg
     4681 cagccaaagc accagcacgt tcagtccgcc caagctggcg ctgtgccgaa acagcgccag
     4741 caggcccacg ccgcacagga ggccgcccat caccgccgcg tacagcggat cgaggcgttc
     4801 gagatcgatc aggcggggca gcatgtccga cagcagcgac agcaggccga tcgccgcgaa
     4861 ggtcttcagc gtgaagcgcc agcccatctt gcgcagcgca agccacgcga acggcaggtt
     4921 caccgcgaag aacagcttgc cgaaaccgat cccgctcaga tagtgcagca gaaacgcgat
     4981 gccgaccgtg ccgcccgtga ccagtcgcgc ctcgccgagc agggtgacgc cgagcgagac
     5041 cagcagcgtg ccggtgagca gcgcctggac atcgtcgagc gcggaatgtc gttgcgggcg
     5101 ggcgggggcg tcaagcggtg aggctggagc
//
LOCUS       DATLGF010000215         6128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_135471, whole genome shotgun sequence.
ACCESSION   DATLGF010000215 DATLGF010000000
VERSION     DATLGF010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135471"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..106)
                     /locus_tag="VM406_06490"
     CDS             complement(<1..106)
                     /locus_tag="VM406_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018057969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HVL75645.1"
                     /translation="MQTLYTAHATASGGREGRATSDDARLDVRLSTPKE"
     gene            complement(222..677)
                     /locus_tag="VM406_06495"
     CDS             complement(222..677)
                     /locus_tag="VM406_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVL75646.1"
                     /translation="MPKSSAPLDPLALDNQLCFALYSASLAMTKAYKPLLEKLGLTYP
                     QYLVMLVLWEQDDVLVKAIGERLFLDSGTLTPLLKRLEAASLVARSRDAEDERQVRIT
                     LTREGRALKKKAQDVPLSILCATGQAPEAIAELRRRLAAIRNDLNAILP"
     gene            914..2224
                     /gene="dacB"
                     /locus_tag="VM406_06500"
     CDS             914..2224
                     /gene="dacB"
                     /locus_tag="VM406_06500"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HVL75647.1"
                     /translation="MERAGIPASAVGIHVAAIEQDTVLLSLNEKTPYNPASVMKLLTT
                     NAALETLGPAFRWTTTAYATGGLADGVLHGDLIIQGSGDPKLVLENFWLFLRRIRAAG
                     VREIRGDLILERGVFAAYPHDPAAFDGDPLRPYNVGPDALLLNFKSLALRFIPDGTAT
                     RVLLEPPLAGFPVQAPHLLEGECGDWRARLSAQIDAGGARFPGTYSASCGEQTWYVHP
                     HPMSHTQYFDALFRRIWTDLGGELRGQVREGPLPADARRIAQWESAPLSELIRDINKF
                     SNNVMARQLLLTMANRMTTLPANPVHGAAVVRTWLGNKGIDAADLVIDNGSGLSREER
                     VSALMLARMLVATWRSPLMPEFVASLPLVGQDGTMRRRLTADPIAGRAHVKTGRLDEV
                     RAIAGYVMAASGRRYAVVCLINHRDVERAHEVQDALLQWVYESG"
     gene            2313..3524
                     /locus_tag="VM406_06505"
     CDS             2313..3524
                     /locus_tag="VM406_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020831369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HVL75648.1"
                     /translation="MAEAVLEAPSTGADRRVETAPAVAAYPEAAAEGEHPFRFTGSGS
                     EYFRIWVVNLLLTILTLGIYSAWAKVRRLQYFHRNTVVAGAIFDYHGEPKAILKGRIL
                     ALVLLLAYQLSTGFSAVAGAIVILLLAAIFPWLLTRAFRFKLANTSYRGLRFRFRGTV
                     GQAYRMLILFPVLFAVIGLFVWSLAASFSNSPSIGLVLLVVLLPMAAIATAVPLAHYC
                     LKRFQHEHADYGQTPFHFSAQARDFFKIYGKSVGLVIVGGAVAGAFFALTAGLFAWLE
                     ASVFGAVFAALYALLGLYVSYLFVRPYMESRMQNLVWNHTELGLHRFESRVKARALLG
                     IHASNLILIVLTLGLYKPFAAVRLARYRVQSMTLVAGADLEDFLADQDADRAGALGQE
                     AGDLFDFDIAL"
     gene            3528..4547
                     /locus_tag="VM406_06510"
     CDS             3528..4547
                     /locus_tag="VM406_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HVL75649.1"
                     /translation="MIHATYFDGRSSRRHPVTVLFHKGVVAIEGENLRRSVRLRQVRI
                     SEPLARAPRILTLPDGAYLESRDAKLDKYLRRNGYRESWVVRWQRNWPASLAALAGLV
                     LLLASGYQWGLPWAADELAQRLPRTIEVKIGEGQLEMLDAGYMEPSRLPPSRQQDLRD
                     AFASLKTPTGQAIPYRLEFRSSRIGPHAFALPNGVIVMTDQMVEMAPDDHAVLAVLAH
                     ELGHLERRHSLRRLLQAAGVGVAVNVYLGDVSSLLALAPAVLLDQGYSRDFEREADRY
                     AIDMMRANGLPLSPIAQLFERLGTPDDDDDRAPHSRSGRSAVIDYLSSHPSDAERIAT
                     LRAAD"
     gene            complement(4568..5155)
                     /locus_tag="VM406_06515"
     CDS             complement(4568..5155)
                     /locus_tag="VM406_06515"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HVL75650.1"
                     /translation="MDRLMRYFTAILLAGMLAGCGYREFQAQDDAVSAAWAEVVRHYQ
                     LRAQLVPELIDTARNASQPATVDAVLQAKSRAAGVDARPEALQNPQLFYAFQQAQGDL
                     SAALAQMLIEVSGDARAETDPALRELQERLHAVENDIIRARERYVVAVREFNKMATRF
                     PTNLTAMALSYELKPTFPVDADRAVTRPPAVAFER"
     gene            complement(5229..6110)
                     /locus_tag="VM406_06520"
     CDS             complement(5229..6110)
                     /locus_tag="VM406_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVL75651.1"
                     /translation="MTALSRKDWILLALLTFFWGINWPIMKLGVTDYPPLTFRTLSML
                     GGLPVIWLAARLQGVSLAVPAGRVMEVVRLAVPNMLIWHVFMILGVSLLTSGRSAILG
                     YTMPVWAVLFALLFFGERPTRPAWIGVGCAFAGALLLLSSEVAAFSGSPLGTLFALIA
                     AAGWGYGTVAMRHSRIEMPTISLTFWMLGLTSVVMVLASWIFESARWTMPDGTTWAAI
                     VYNALIVFGFAHVVWFQLARMLPPFISGLSVMMIPVLGVFSGAWWLNETLHWQDFAAM
                     LLILVAMSTVLLKPRLR"
BASE COUNT         1019 a         2022 c         2037 g         1050 t
ORIGIN      
        1 gttccttcgg tgtgctgagc ctgacgtcga ggcgggcgtc gtcgctggtg gcgcggcctt
       61 cacggccgcc gctggcggtg gcatgtgcgg tgtaaagcgt ttgcatcgtt gttctccatt
      121 ctggttgagt cggcaaggcg ccgcgcgaga acaatagcac acaattgaat tgcgtgcgat
      181 gcaagtcgcc ggacggcatt gtcggtgctt cgacaccagc gtcagggaag gatggcgttg
      241 aggtcgttgc ggatggcggc caggcggcgg cgcagctcgg cgatcgcttc cggcgcctgg
      301 ccggtcgcgc acaggatgct gagcggcacg tcctgcgcct tcttcttcag cgcccggcct
      361 tcgcgcgtca acgtgatgcg gacctggcgc tcgtcctcgg catcgcggct gcgcgcgacc
      421 agactagccg cctcgagccg cttcaagagt ggcgtgagcg ttcccgaatc gaggaacagg
      481 cgctcgccga tcgccttcac cagcacgtca tcctgctccc acagcaccag catcaccagg
      541 tactgcgggt aggtgagccc gagcttttcg aggaggggct tgtacgcctt ggtcatcgcg
      601 agcgacgcgg agtacagcgc gaagcagagc tggttgtcga gcgcgagcgg gtcgaggggg
      661 gcggatgatt tcggcatgat aaaacggcag tgtgcgagaa ccggattccc ggctgaccgg
      721 ggagcgtcag ggaaggggcc ggtggctgaa caatccgcac gccttctttc tggacagcga
      781 ttgaattgcg tgcgacatta tttcgcatcc tatgcgaatt ccgaacagac tccaagccat
      841 cctcgctgcc gccgctttcg ttgttcatgc ggcaagcagt gcatccgctc tgcctcccac
      901 cgtatcgagc gcgatggagc gtgccggcat ccctgcatcc gccgtcggca tccacgtcgc
      961 cgcaatcgag caggataccg tcctgctgtc gctgaacgag aaaacaccgt acaacccggc
     1021 gtcggtgatg aagctgctca ccaccaatgc cgcgctggag acgctgggtc cggcgttccg
     1081 ctggacgacc accgcctacg cgaccggcgg gctggccgac ggcgtcctgc acggcgacct
     1141 gatcatccag ggcagcggcg atcccaagct ggtgctggag aatttctggc tgttcctgcg
     1201 ccgcatccgc gcggccggcg tgcgcgagat ccgcggcgac ctgatcctcg agcgcggcgt
     1261 gttcgcggcc tatccgcacg acccggccgc gttcgacggc gacccactgc gtccatacaa
     1321 cgtcggcccg gatgcgctgc tgctcaactt caagtcgctc gcgctgcgct tcatacccga
     1381 cggcacggcc acacgcgtgc tgctcgagcc gccgctggcc ggttttcctg tgcaggcgcc
     1441 gcacctgttg gaaggcgaat gcggcgactg gcgtgcccgc ctgagcgcgc aaatcgatgc
     1501 cggcggcgcg cgctttccag gaacgtactc ggcgtcctgc ggcgagcaga cctggtatgt
     1561 gcatccgcac ccgatgtcgc atacccagta ttttgatgcg ctgtttcgcc gcatctggac
     1621 cgatctcggc ggcgagctgc gcggacaggt gcgcgaaggc ccgctgccgg cggatgcgcg
     1681 tcggatcgcg caatgggaat cggcaccgct gtccgaactg atccgcgaca tcaacaagtt
     1741 cagcaacaac gtgatggcgc gccagctgtt gctgaccatg gccaaccgca tgaccacctt
     1801 gccggccaat cccgtgcatg gtgcggcggt ggtgcgcacc tggctgggca acaagggcat
     1861 cgacgcggcg gatctcgtga tcgacaacgg ctccggcctg tcgcgcgagg agcgggtgtc
     1921 ggcgctgatg ctcgcgcgca tgctggtggc gacctggcgc tcgccgctga tgcccgaatt
     1981 cgtcgcctcg ctgccgctgg tcgggcagga cggcacgatg cggcgcaggt tgacggccga
     2041 tccgattgct ggacgcgccc acgtcaagac cgggcggctg gacgaggtgc gcgcgatcgc
     2101 cggctacgtg atggcggcgt ccggcaggcg ctacgccgtg gtgtgcctga tcaaccaccg
     2161 cgatgtcgaa cgcgcccacg aggtgcagga cgcgctgctg cagtgggtgt acgagagcgg
     2221 ctgaaaccgc ttctggctgg tgtattcttg gcaacaaagc gcggaccttt ccgccaacaa
     2281 caagcacacc gccgacacat caggaaggga acatggcaga agccgtactg gaagcgccgt
     2341 caaccggcgc ggaccggcgc gtggagacag cacccgcggt ggcggcgtat cccgaggcgg
     2401 cagccgaagg cgaacacccg tttcgcttca cgggcagcgg ctccgaatac ttccgcatct
     2461 gggtcgtcaa cctgctgttg accatcctga cgctcggcat ttattcggcg tgggcgaagg
     2521 tgcgccggct ccagtatttc catcgcaaca ccgtcgtggc gggcgccatt ttcgattatc
     2581 acggcgagcc gaaggcgatc ctgaaggggc gcatcctcgc gctcgtgctc ctcctggctt
     2641 accagctctc gactggcttt tccgccgtgg cgggcgcgat cgtgattctg ctgctggcgg
     2701 cgatcttccc gtggctgctc acccgcgcgt ttcgcttcaa gctcgccaac accagctacc
     2761 gcggactgcg ctttcgcttt cgcggcacgg taggccaggc ctaccggatg ctgatcctgt
     2821 tcccggtgct gtttgcggtc atcggcctgt tcgtctggag cctggcggca agcttctcca
     2881 attcgccgag cataggcctg gtcctgctgg tggtgctgct gccgatggcg gcgattgcga
     2941 cggccgtgcc gctcgcacac tactgcctga agcgctttca gcacgaacac gcggactacg
     3001 ggcagacgcc gttccacttt tcggcccagg cgcgcgactt cttcaagatc tacggcaaat
     3061 cggtcggtct cgtcatcgtc ggcggcgctg tggccggcgc cttctttgcg ctgacggcgg
     3121 ggctgtttgc gtggctggag gcgagcgtat ttggcgccgt gttcgccgcg ctgtacgcat
     3181 tgctcggcct gtacgtgtcc tacctgttcg tgcggcccta catggaaagc cggatgcaga
     3241 acctggtgtg gaaccacacc gagctcgggc tgcaccgttt cgagagccgc gtgaaggcgc
     3301 gcgcgctgct gggcattcat gcgtccaacc tcattctcat cgtgctcacg ctcggcctgt
     3361 acaagccgtt cgccgcggtc cggctggcac gctaccgggt ccagagcatg accctggtgg
     3421 ccggggcgga cctcgaggac ttcctcgccg accaggacgc cgatcgggcc ggcgcgctcg
     3481 gccaggaagc cggcgacctg ttcgatttcg acatcgcact ctaagccatg atccacgcca
     3541 cctacttcga cggccgcagc agccgccgcc atcccgtcac cgtgctgttc cacaaggggg
     3601 tcgtcgcgat cgagggcgag aacctgcgcc gcagcgtccg cctgcgccaa gtgcgcatct
     3661 cggagccgct agcacgcgcc ccgcgcatcc tgaccctgcc ggatggcgcc tatctcgaat
     3721 cgcgcgacgc caagctggac aaatacctgc gccgcaacgg ctatcgcgag tcctgggtgg
     3781 tgcgctggca gcgcaactgg ccggcctcgc tggcggcgct tgccggcctg gtcctgctgc
     3841 ttgcgtccgg ctaccagtgg ggcttgccgt gggctgcgga cgaactggcg cagcgcctgc
     3901 cacgcacgat cgaagtgaag attggcgaag gtcagctcga gatgctcgat gccggctaca
     3961 tggaaccgag ccgtctgcct ccatcgcgcc agcaggattt gcgcgacgcc ttcgcgagct
     4021 tgaagacacc gaccgggcag gcgatcccgt atcgcctcga gttccgctcc agccgcatcg
     4081 gcccgcacgc attcgcgcta ccgaacggcg tgatcgtgat gaccgaccag atggtggaga
     4141 tggcgccgga cgaccatgcg gtgcttgcgg tgctggcgca cgaactcggg cacctggagc
     4201 ggcgccattc gctgcgccga ctgctgcagg ccgccggcgt cggcgtggcg gtgaacgtgt
     4261 atctgggcga tgtctccagc ctgctcgcgt tggcgccggc cgtgctgctg gaccagggat
     4321 attcgcgtga cttcgagcgg gaggccgacc gctatgcgat cgacatgatg cgcgccaacg
     4381 ggctgccgct gtcgccaatc gcgcagctgt tcgaacgcct gggcacgccg gatgatgacg
     4441 atgaccgtgc gccgcattcg cgctccggcc gcagcgcggt gatcgactac ctcagctcgc
     4501 atccgagcga cgcggagcgc atcgcgacgc tgcgcgcggc ggattagcgc atcagtgcat
     4561 cagcgcatca gcgttcgaac gccaccgcag gcgggcgcgt gaccgcacgg tcggcgtcga
     4621 ccggaaaggt cggcttgagc tcatagctca gcgccatcgc cgtcagattg gtgggaaatc
     4681 gcgtcgccat cttgttgaat tcgcgtaccg cgaccacgta gcgttcgcgc gcgcggatga
     4741 tgtcgttttc gaccgcgtgc agccgctcct gaagttcgcg gagggcgggg tcggtttccg
     4801 cgcgggcatc gccggaaacc tcgatcaaca tctgagccag tgccgccgac aaatctccct
     4861 gggcctgctg gaacgcgtaa aagagctgcg gattctgcag cgcttccggg cgggcgtcga
     4921 cgccggccgc gcgggacttt gcctgcagca ccgcgtccac cgtcgcaggt tgcgaggcgt
     4981 tcctggctgt gtcgatcagc tccggaacca gttgcgcgcg caactggtaa tgccgcacca
     5041 cttcggccca ggctgcggaa accgcgtcat cctgggcctg gaattcacgg tagccgcagc
     5101 cggccagcat gccggccaac aatattgccg tgaaatagcg catcagcctg tccatgccgt
     5161 cccctgcctg cgtctagcgg ttccctgaag agagacgccg gacgatgccg aacgttcacg
     5221 gccgacgttc agcgcaggcg cggcttcagc aggaccgtgc tcatcgcgac gaggatcagc
     5281 agcatcgccg cgaagtcctg ccagtgcagg gtttcgttca gccaccaggc gccggagaat
     5341 acgccaagca ccggaatcat catgaccgac aggccggata tgaacggcgg cagcatgcgc
     5401 gcaagctgga accagaccac gtgggcgaag ccgaacacga tcagcgcgtt gtacacgatt
     5461 gcggcccagg tcgtgccgtc cggcatggtc cagcgcgcgc tttcgaagat ccaggaagcg
     5521 agcaccatca ccacgctcgt caggccaagc atccagaacg tcagcgagat ggtcggcatc
     5581 tcgatgcggg aatggcgcat cgcgaccgtg ccgtagcccc agccggccgc ggcgatgagg
     5641 gcgaacagcg tgccgagcgg gctgccggag aacgccgcga cctcgctgga gagcagcagc
     5701 agcgcgccgg cgaatgcgca gccgacgccg atccacgccg ggcgtgtcgg ccgttcgccg
     5761 aagaacagca gcgcgaacag caccgcccac accggcatcg tgtagccgag gatggccgag
     5821 cgccccgagg tcaagaggct cacgccgagg atcatgaaga cgtgccagat cagcatgttg
     5881 ggcaccgcca gccgcaccac ctccatcacg cgcccggccg gaaccgccag agagacaccc
     5941 tgcaggcgcg cggccagcca gatcaccgga agcccgccca gcattgacag cgtgcggaag
     6001 gtaagcggcg ggtagtcggt gacgccgagc ttcatgatcg gccagttgat gccccagaag
     6061 aatgtcagca gggcgaggag aatccagtct ttgcgtgaga gggcagtcat gcagcggcag
     6121 cggtagtg
//
LOCUS       DATLGF010000216         4285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_87708,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000216 DATLGF010000000
VERSION     DATLGF010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87708"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="VM406_06525"
     CDS             complement(<1..649)
                     /locus_tag="VM406_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HVL75652.1"
                     /translation="MQNAGKFDYIIIGAGTAGCVLAKRLVQEAGASVLLLEAGRKDNY
                     IWIHIPVGYLYCIGNPRTDWLFRTEADPGLNGRSLIYPRGKVLGGCSSINGMIYMRGQ
                     VRDYAEWAQLTGDDSWRWENVLPVFKQSEDYHKGADEFHGAGGEWRVEKQRLSWEILD
                     AFREAAQATGIPMTEDFNRGDNEGCGYFEVNQKQGLRWNTARAFLKTAQGAPGRLE"
     gene            complement(978..2486)
                     /locus_tag="VM406_06530"
     CDS             complement(978..2486)
                     /locus_tag="VM406_06530"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HVL75653.1"
                     /translation="MKRIAHYINGRVAESSSGRSQDVYNPATGEVSGQVVLGSVEEVG
                     QAVAAAKAAAPAWAETAPLKRARILSKFKELIETHHDALAAAITREHGKVFSDAKGEV
                     VRGLEVVEFACGVPQLLKTQFTDNIGGGIDNWQLRQPLGVTAGITPFNFPFMVPMWMA
                     PMALATGNTFVLKPSERDPSPSLMVAEMLHDAGLPDGVFNVVQGDKVAVDALLQHPDV
                     QAVSFVGSTPIAEYIYTEGVRRKGAYPLRVQALGGAKNHLVVMPDADLDQAVDALIGA
                     AYGSAGERCMAISVAVAVGSIADRLVEALVPRVQALKIRNGMEADAEMGPLVTAQHKA
                     KVEGYIAAGVEEGAKLLVDGRGLKVPGHENGFFLGGSLFDHVRPGMKIYQEEIFGPVL
                     CVVRVPDFAAAVELINAHEFGNGVSLFTSDGNTAREFSRRIEVGMVGINVPIPVPMAW
                     HSFGGWKRSLFGDLHAYGEEGVRFYTRYKSVMQRWPASIGKGAEFTMPVAKG"
     gene            2587..3462
                     /locus_tag="VM406_06535"
     CDS             2587..3462
                     /locus_tag="VM406_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL75654.1"
                     /translation="MDFTQLRAFVTVAREGNLTRAAERLHLTQPAVSLQIKGLQSALG
                     LQLFMRTPSGMSLTDDGAKLLPFAERVMEAVAEFRQGASSLHSTLSGSLAIGTILDPE
                     FIRLGAFLKRLVESHPQLSTRLQHGMSGTVLQQVRSGELDGGFYIGTPGRDFHHLVLT
                     SFTYSVVAPHGWKQRVAGRDWPALAALPWIWTPPESAHHRMLTRTFAHYKVTPNKVAL
                     VDQEPSMLDLVKSGVGLSLVRESIALREAHAHGLVIADAVSLSTELSFVCLARRRGDP
                     VLAAVFGLLENLWKT"
     gene            3909..>4285
                     /locus_tag="VM406_06540"
     CDS             3909..>4285
                     /locus_tag="VM406_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HVL75655.1"
                     /translation="MRLGIISALQQEQAGLIEAMTDVSVEQRGMRSYTCGTLWGQEAV
                     CVLARIGKVAATATVATLIERYQVTHVVFTGVAGAIDSELRPGDVVIADTLVQHDLDA
                     SPLFPRFEVPLLGLAHFSSDRHLS"
BASE COUNT          743 a         1403 c         1324 g          815 t
ORIGIN      
        1 cctccagccg tcccggcgca ccctgcgctg tcttcaggaa cgcgcgggcg gtattccagc
       61 gcaacccctg cttctggttc acctcgaagt agccgcagcc ttcgttgtcg ccgcggttga
      121 agtcctcggt catcgggatg ccggtcgcct gcgccgcttc ccggaacgcg tcgaggattt
      181 cccaggacag ccgctgcttt tcgacccgcc attcgccgcc ggctccgtgg aattcatccg
      241 cgcccttgtg atagtcctcg ctctgcttga ataccggcag cacgttctcc cagcgccagc
      301 tgtcgtcgcc ggtcagctgt gcccattccg cgtagtcgcg cacctgtcca cgcatgtaga
      361 tcatgccgtt gatcgacgag cagccgccca gcaccttgcc gcgcgggtag atcaggctgc
      421 gcccgttcag tccgggatcg gcttcggtgc ggaacagcca gtcggtgcgc ggattgccga
      481 tgcagtacag atagccgacc ggaatatgga tccagatgta gttgtccttg cggcccgctt
      541 cgagcagcag caccgatgca ccggcttcct gcacgaggcg cttggccagc acgcagccgg
      601 ccgtgccggc gccgatgatg atgtagtcga acttgcctgc gttttgcatg gaggtctttc
      661 gctggtgagt ctggtcttgc agtggctggt ggtgaaagcg ggcggttttt aatctgtatt
      721 gttctatata tacatagtaa tgatggttaa cacccctgcc cgcctgtgga taaccatggt
      781 ttaccgtttc cgttcaagct cttgccgcaa gcataagcgg acgtacaacg catgtatgag
      841 cgaaggacgg ttcttggaga aaatttgcgc gccggacttg ttcgtccctt ctgcacaacc
      901 ggcccagtgg aagtgcagag gcttatccac aggcggggcc gccgtcatac agccatgacg
      961 gcgacggcgg accaaggcta ccccttcgcg accggcatcg tgaattccgc acccttgccg
     1021 atcgacgccg gccagcgctg catcaccgac ttgtagcgcg tatagaagcg cacgccttcc
     1081 tcgccgtacg catgcaggtc gccgaacagc gagcgcttcc agccgccgaa cgaatgccag
     1141 gccatcggca ccgggatcgg cacattgatg ccgaccatgc caacctcgat gcggcgcgag
     1201 aattcccgcg cggtattgcc gtccgaggtg aacagcgaca cgccgttgcc gaattcatgc
     1261 gcgttgatca gctcgaccgc cgcggcgaaa tccggcacgc gcaccacgca cagcaccggt
     1321 ccgaagattt cctcctggta gatcttcatg cccggccgca catgatcgaa cagcgaaccg
     1381 ccgaggaaga agccgttctc atggccaggc accttcagcc cgcggccgtc gaccagcagc
     1441 ttcgccccct cctccacgcc ggcggcgata tagccctcga ccttggcctt gtgctgggcg
     1501 gtcaccagcg ggcccatttc ggcgtcggct tccatgccgt tcctgatctt cagcgcctgc
     1561 acgcgcggca ccagcgcctc gaccagccgg tcggcgatcg agccgaccgc caccgcgacg
     1621 gagatcgcca tgcagcgctc gccggccgag ccataggccg cgccgatcag cgcatcgacg
     1681 gcttgatcga gatccgcgtc cggcatcacc accaggtggt tcttggcgcc gcccaaggcc
     1741 tgcacgcgca gcggatacgc gcccttgcgg cgcacgccct cggtgtagat gtactcggcg
     1801 atcggcgtgg agccgacgaa agatacggct tgcacatccg gatgctggag cagcgcgtcg
     1861 accgccacct tgtcgccctg cacgacgttg aacacgccgt ccggcaggcc ggcgtcgtgc
     1921 agcatctcgg caaccatcag cgacggcgac ggatcgcgtt cggacggctt gagcacgaag
     1981 gtattgccgg tggccagcgc catcggcgcc atccacatcg gcaccatgaa agggaaattg
     2041 aacggcgtga tgccggccgt cacgccgagt ggctggcgca gctgccagtt gtcgatgccg
     2101 ccgccgatgt tgtcggtgaa ctgggtcttg agcagctgcg gcacgccgca cgcgaattcc
     2161 accacctcca gtccgcgcac gacttcgccc ttggcgtcgg agaacacctt gccgtgctcg
     2221 cgcgtgatcg ccgcggccag cgcgtcgtgg tgggtctcga tcagctcctt gaacttggac
     2281 aggatgcgtg cgcgcttcaa cggcgcggtc tcggcccagg ccggcgcggc tgccttcgca
     2341 gccgccaccg cctgccccac ctcttccacg ctgcccagca cgacctggcc actgacctcg
     2401 ccggtggcgg ggttgtacac atcctggctg cggccggagc tcgattcggc cacgcggccg
     2461 ttgatgtagt gggcaatacg cttcataagg attcctttgg cgtgatgggt gtttttgcca
     2521 gaatatccgc gctcgggagc gaaatccaat gagatatcat caagatcttc ataaggaagt
     2581 ctaataatgg atttcaccca attgcgtgcc ttcgtgacag tcgcgcggga aggcaacctc
     2641 acccgcgccg ccgagcggct gcatctgacc cagccggcgg tcagcctgca gatcaaggga
     2701 ctgcagtccg cgctcggcct gcagctgttc atgcgcacgc cgtccggcat gagcctgacc
     2761 gacgatggcg ccaagctgct gccgttcgcc gagcgggtca tggaggcggt ggccgaattc
     2821 cgccagggcg catcgtcgct gcattccacg ctgtccggat cgctcgcgat cggcacgatt
     2881 ctcgatccgg aattcatccg cctcggcgcc ttcctcaagc gcctggtgga aagtcatccg
     2941 caactgtcga cccggctgca gcacggcatg tccggcaccg tgctgcagca ggtgcgcagc
     3001 ggcgagctcg acggcgggtt ctacatcggc acgccggggc gcgacttcca ccatctggtg
     3061 ctgacctcgt tcacctacag cgtggtcgcg ccgcatggct ggaaacagcg ggtggccggc
     3121 cgcgactggc cagcgctggc cgcgctgccc tggatctgga cgccgccgga atccgcccat
     3181 caccggatgc tcacccgcac cttcgcgcac tacaaggtca cgccgaacaa ggtcgcgctg
     3241 gtcgatcagg aaccgtcgat gctggatctg gtgaagtccg gggtggggct gtcgctggtg
     3301 cgcgaatcga tcgcgctgcg cgaagcgcat gcacacggcc tggtgatcgc cgatgcggtc
     3361 agcctgtcca ccgaactgtc cttcgtctgc ctcgcgcgcc ggcgcggcga tccggtgctg
     3421 gcggcggtgt tcggcctgct ggagaacctc tggaaaacct gatgtggata agtggcggga
     3481 aaacgctggg gtgtattggg gaaaaatgcc gacagatgtg aacaggtttt tttgtgcaga
     3541 aaccatcctt gcgccataca aggtttttac ggcagttgtg cgcggtgtat cccgttgagc
     3601 aggaatggaa aaatccagtt atccacagga aggcgaccgt ttaccactac tactatttat
     3661 atatatcaac agcaatataa aacccacggc atctcgggcg gggtcgcgca gggcggatgg
     3721 caatacggat gcaaccggat gagatccctc gcttcgctcg ggatgacagg taggtgcggg
     3781 gttgtcgccg tgatccgccg tgttcgccgt ggtgagaacg gccggtaatt cggtgtctac
     3841 gctcaccttg gcgctctgcc ccaaacccgt tacgctctga agcttcacct tccacatctc
     3901 cacatcccat gcgcctcggc atcatcagtg cattgcaaca ggaacaagcc ggattgatcg
     3961 aggcgatgac cgacgtgagc gtcgagcagc gcggcatgcg cagctatacc tgtggaacgc
     4021 tgtggggaca ggaagcggtg tgcgtgctcg cgcgcatcgg caaggtcgcg gcgacggcga
     4081 ccgtcgccac gctgatcgag cgctaccagg tcacgcacgt cgtcttcacc ggcgtggccg
     4141 gcgcgatcga cagcgaactg cggccgggcg acgtggtgat cgccgacacc ctggttcagc
     4201 acgatctcga tgcgagtccg ctgtttcccc gtttcgaggt gcccttgctg ggcttggcgc
     4261 atttttcatc ggaccggcat ctgtc
//
LOCUS       DATLGF010000217        10890 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_116050, whole genome shotgun sequence.
ACCESSION   DATLGF010000217 DATLGF010000000
VERSION     DATLGF010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 10890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10890
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116050"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VM406_06545"
     CDS             <1..205
                     /locus_tag="VM406_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HVL75656.1"
                     /translation="RTALPGVLAVEMEGAAVAQVCFEFGVPFAVMRTISDSADEAAPI
                     DFVRFVERIASPYAYGILQGLLK"
     gene            complement(226..447)
                     /locus_tag="VM406_06550"
     CDS             complement(226..447)
                     /locus_tag="VM406_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75657.1"
                     /translation="MDEEIVLARDRDGYRVLAGRHRLDVVLRDSDEAFADVAGERGRA
                     KVFRTPAGLLVCKDSRYLPLIDASAAAAP"
     gene            complement(747..4433)
                     /gene="putA"
                     /locus_tag="VM406_06555"
     CDS             complement(747..4433)
                     /gene="putA"
                     /locus_tag="VM406_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HVL75658.1"
                     /translation="MIAMLTETRAARPAFSAFFDDIAQAPSPLRAAITAAWRRDEPEA
                     VQWLLAQVPGDAQARPQAEALARRLVAAVREKRTRASGVDALMHEFSLSSEEGVALMC
                     LAEALLRIPDKETADRLIADKISRGDWKRHLGESPSLFVNAATWGLLITGKLVSTSSE
                     QGLSSAITRLLAKGGEPLIRKGVDLAMRMLGNQFVTGQTIEEALKNSRDNEKRGYRYS
                     YDMLGEAALTAEDAAKYYAAYEHAIHAIGRASGGRGIRNGPGISVKLSALHPRYSRAQ
                     RERVMAELLPRLKALLLLARQYDTGLNIDAEEADRLELSLDLMEALAFDADLAGFDGI
                     GFVVQAYQKRCPFVIDYLVDLGRRSGRRFMVRLVKGAYWDAEIKRAQVDGMPGYPVYT
                     RKIHTDVSYLACARKLLAASDVIYPQFATHNAHTLASIHTWAQEQRVDDYEFQCLHGM
                     GETLYDQVVGADKLGKPCRIYAPVGSHETLLAYLVRRLLENGANSSFVNQIVDENVSI
                     DQLVANPFEGARQSGGAPHPNIALPVDLFGAERRNSMGVDLANESVLQALGHAFRNFS
                     THQWSAAPLIAGTVSATTAQAVRNPADGRDLVGRVVEADAQDVETALAAATAYAASWE
                     STPAGQRAALLERAADLLEAQREEFMALAVREAGKSLPNAIAEVREAVDFLRYYAAQV
                     RQETPGPALGPVVCISPWNFPLAIFVGEVSAALAAGNVVLAKPAEQTPLIAHRAVELL
                     HAAGIPRAALQFLPGRGETVGARLTADPRTRGVIFTGSTEVAQLINRTLAARSAEEGQ
                     DIALIAETGGQNAMIVDSSALPEQVVQDVLASAFDSAGQRCSALRVLCLQADIADRTI
                     AMLKGAMRELKIGNPDHLAVDVGPVIDADAQRGLLRHIDGMRGSAREVFQLGLPPECE
                     HGTYVAPTVIEIASLAQLEREVFGPVLHVLRYRREELPALVDAINATGYGLTLGVHSR
                     IDETIDFVVSRAHVGNIYVNRNIIGAVVGVQPFGGEGKSGTGPKAGGPLYLKRLQRNA
                     KVARAAHTIHAGSGGNGSARALQALMQWAGANGHAAQAALANEYAHASLLDTTLPLPG
                     PTGERNTLAFAPRGRILCAAGDCASLLNQIAACLATGNLAAVAPSVVALLPADLPDAV
                     RACVLPLAADALQQGDFQLALVDQGQRGSVLPLLAARNGPLVMAVDTTADEAIALWRL
                     AAERAVCVNTTAAGGNASLMTLGS"
     gene            4567..5025
                     /locus_tag="VM406_06560"
     CDS             4567..5025
                     /locus_tag="VM406_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HVL75659.1"
                     /translation="MLDKISKKILSELQNDGRISNVELASRVNLSPAACLERVRKLQE
                     AGYILHYTAQLNPQLLDVSLLVFIEVVLDRTTPEVFEAFKRSVQVIPEVQECHMVAGG
                     FDYLVKARVKDMNAYREFLGKSLLQLKGVRETHTYAVMEEVKNTTKLPIR"
     gene            5036..5344
                     /locus_tag="VM406_06565"
     CDS             5036..5344
                     /locus_tag="VM406_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HVL75660.1"
                     /translation="MLELRPGCEHCDTPLPPEATNAMICSFECTFCADCVSKVLSNVC
                     PNCAGGFWPRPVRPRVNRKGGNFLGNYPAGTRVVHKPVDSEAHAELVARCASIPPAQR
                     "
     gene            5465..7672
                     /locus_tag="VM406_06570"
     CDS             5465..7672
                     /locus_tag="VM406_06570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL75661.1"
                     /translation="MRIRTRLLLLVLAILLPASLCAVVALAYAYHETQDFQRQAMRET
                     ARALSLGLERELARRESILHALAASPALDNDDFRNFYAHAKQIADAGDVAIFLSTSDG
                     RQLLNTRVPYGKPLPRMLPQALEMRAEAGPRATLISNLYEPPVGKGYSFAIQIPVERN
                     GRVRYYLNMGSYAAQLQSLLELQRLPPEWIAGIADAHGVIVARNRDAERYVGVSVRRT
                     VGHLLEAGEGFAEGRALSGEAATVFLSHAPHYGWNFYVAVPTDLLQRAAWRTNAWLAL
                     AMLLLLGLALLAALKMARDTAGAIEATRRAAQRLGERRPVRVQPSGLVELDTVNAALA
                     EAGTSLQAAHEDQERRIADAVASAERSQRALLHAQKLEALGRLTGGIAHDFNNILQTV
                     STGVELTRRLTTDARALGTLDNCARAVRRAAELTRQLAVFGRRQDSRIETIDPVRHLT
                     EVRPLLAGGLRSDIELNVDIPTSLWHITVDPLQFELALLNLTMNARDAMPAGGKLRLC
                     AHNETLTVPLDELQPGDYVRISLLDNGEGMTPEVLARAIDPFFTTKGVGQGTGMGLSQ
                     AYGFARQSGGTLLLQSQSGKGTDAILYLPRALQMPITPAVAGSGGSKRRADGERVLLV
                     DDDAQVREVVRAALESRGFSVLEAEDGDEAIRMLQSGIAVDAVLSDIVMPGSLSGIDL
                     AHQLHAEHPNVAVLLTTGYSERRIDLRGIRTLPKPYSTDDAAEALLDALAEVR"
     gene            7756..8106
                     /locus_tag="VM406_06575"
     CDS             7756..8106
                     /locus_tag="VM406_06575"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HVL75662.1"
                     /translation="MSADPSLLSGKREELALLRFGPLEGRVLHALWAFHEVTLADLHH
                     LLGDAALTPTVLRATLERLHLKRLIRLRKVHRTCFYRAALDQSTFISLLHHQLAGFLG
                     EEGMSQLRHLGASV"
     gene            8217..9194
                     /locus_tag="VM406_06580"
     CDS             8217..9194
                     /locus_tag="VM406_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HVL75663.1"
                     /translation="MNMRKWIPAAGLVAAAMSNVVFAQAVQVDPALADYQKAAGVSGN
                     FTSVGSDTLNNLMTLWAEEFKKTYPNVNIQIQGAGSSTAPPALTEGASNFGPMSRMMN
                     AKEIESFERRHGYKPTAVPVAIDALAVYVHKDNPIKGLSLEDVDAMMSVARKCGSAAD
                     VTKWGQIGMSGEWANRDVAMYGRNSVSGTYGFFKDTALCKGDFKRNVAEQPGSASVVQ
                     SVSTQINAIGYSGIGYKTSGVRALPLSKKRGEAFVEPDAAHAVDGSYPLARILYVYVN
                     KKPNQPLAPIEREFVKLVLSKQGQQVVVKDGFVPLPARIANQALEQLVK"
     gene            9255..9860
                     /locus_tag="VM406_06585"
     CDS             9255..9860
                     /locus_tag="VM406_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75664.1"
                     /translation="MNKTIKLAALASGMFAALVLAGCAAPTQQAQAPAAPAAAAAPAA
                     PAAVAAAREFYVVLPEDGRYYAFADTKNYFGFLAHAEVPLTRTRIGAGPGGKTVVFGI
                     SGDDVKNNRPSMAEQVFDGKLQADANFYGEVFKNGRFYVFGNMKDMMDFAQFGEVPYS
                     FTDIGAGPKGETLVWVMNKDSYAKGRPVTTIAQFQKVRTVQ"
     gene            10144..>10890
                     /locus_tag="VM406_06590"
     CDS             10144..>10890
                     /locus_tag="VM406_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease"
                     /protein_id="HVL75665.1"
                     /translation="MNTLTRSQTAVTMDRAMHRRLVTDKVFKVLMSAGGIGVIVAVSA
                     IFFYLASVVVPLFVPPSVEAQASFAAPGSAVQRTGAYSGEEQREIGVRIGATGDVSFF
                     GFKDGRVLQESRITMPAGAQLTSFALADRSSYAFAYGLSDGRAVVAKQAYKVTFPDNK
                     RLITPGIEYPLGEDAVVIDPRGSALQQLAVQHGDSGTTLAGYTADGRLLVSAVTVTTN
                     LMTGESRSEAAQAEVPGAPGTSAWAASLRDS"
BASE COUNT         1860 a         3611 c         3573 g         1846 t
ORIGIN      
        1 gcgcactgcg ctgcctggtg tgctggcggt cgagatggaa ggcgcggcgg tggcgcaggt
       61 gtgcttcgaa ttcggcgtgc cgttcgccgt catgcgcacg atttccgaca gcgcggacga
      121 ggccgcaccg atcgacttcg tacgctttgt cgagcgcatc gcttcgccgt atgcctacgg
      181 catcctgcag ggcttgctga aatagcagct tcagggataa cagcttcagg gcgcggccgc
      241 agccgaagcg tcgatcagcg ggaggtaacg gctgtccttg cagacgagca ggccggcggg
      301 cgtgcggaac accttggcgc gcccgcgttc gcccgccaca tcggcgaacg cttcatcaga
      361 atcgcgcaag acgacgtcga gccgatggcg tcccgccagc acgcggtagc cgtctcggtc
      421 gcgggcaaga acgatttcct cgtccatgtc tcctcccccg aatgcaatgc agaaggccgg
      481 caacagcgcc gtagctgttc atcttgcgat gaccctcgcc atgtcgttcc cgcgaaggcg
      541 gaaacccagc ccgggcgtct ggagccacgc tgaagccgga gctgggtgca gcaggtgtga
      601 gcgcttcgat aaacggcgtt tcgcatcaag tcccggcctt cgactggttc ttgatgttgg
      661 acggtcaatc tacagcgaac agcagtcatg cgggcgatct ggaaacgcga ttgcacgcac
      721 tgttgagctg cgtcaactga cgcaaatcag cttccgagtg tcatcaggct cgcattgccg
      781 ccggctgcgg tcgtgttcac gcagaccgcg cgttccgcag ccaggcgcca cagcgcgatg
      841 gcttcgtcgg cggtggtgtc gaccgccatc accagcggac cgttgcgcgc ggcgagcagc
      901 ggcagcacgc tgccgcgctg cccctggtcc accagcgcga gctggaagtc tccctgctgc
      961 agcgcatcgg ctgccagcgg cagaacgcag gcgcgaaccg cgtccggcag atccgccggc
     1021 agcagcgcaa cgacggatgg agcgactgcg gccaggttgc cggtggcgag gcaggcggca
     1081 atctggttga gcaggcttgc gcaatcgccg gccgcgcaca ggatgcggcc gcgcggagcg
     1141 aaggccagcg tgttgcgttc gccggtcggc ccgggcagtg gcagcgtggt gtcgagcagc
     1201 gatgcatgtg catactcgtt tgccagcgcc gcctgcgcgg cgtggccgtt ggcgccggcc
     1261 cattgcatca gcgcttgcag cgcgcgggcg gaaccgttgc cgccgctgcc cgcatggatc
     1321 gtgtgcgccg cgcgcgcgac cttggcgttg cgctgcagac gcttcaggta gagcggacct
     1381 ccggctttcg ggccggtgcc ggacttgccc tccccgccga acggctgcac accgaccacc
     1441 gcgccaatga tgttgcggtt cacgtagatg ttgccgacat gggcacggct gacgacgaag
     1501 tcgatggtct cgtcgatgcg cgaatgcacg ccgagggtca gcccgtagcc ggtcgcgttg
     1561 atcgcgtcga ccagcgccgg cagttcctcg cgccggtagc gcagcacgtg cagcaccggg
     1621 ccgaacactt cgcgctcgag ctgcgcgagc gacgcgatct cgatcacggt cggcgccaca
     1681 taggtgccgt gctcgcattc cggcggcagc cccagctgga acacttcgcg cgcactgccg
     1741 cgcatgccgt cgatgtggcg cagcaggccg cgctgcgcgt cggcgtcgat gaccgggccg
     1801 acgtccacgg ccaggtgatc cgggttgccg atcttcagtt cgcgcatcgc acccttgagc
     1861 attgcgatcg tgcggtcggc gatgtcggcc tgcaggcaca gcacgcgcag cgccgagcaa
     1921 cgctggccgg cgctgtcgaa ggccgaggcg agcacgtcct gcaccacctg ttccggcagc
     1981 gcggaagagt cgacgatcat cgcgttctgc ccgccggtct cggcaatcag cgcgatgtcc
     2041 tgcccttcct cggcgctgcg cgcggccagc gttcggttga tcagctgggc cacttcggtg
     2101 gagccggtga agatcacgcc tcgggttcgc ggatcggcgg tcagccgcgc accgacggtt
     2161 tcgccgcggc cgggcaggaa ttgcagcgcg gcacgcggga tcccggccgc gtgcagcagt
     2221 tcgaccgcgc ggtgcgcgat cagcggcgtc tgctcggccg gcttggccag caccacattg
     2281 ccggccgcca gcgccgcgct gacttcgccg acgaagatcg caagcggaaa attccatggg
     2341 ctgatgcaca cgaccggccc gagcgccgga ccgggcgtct cctgcctgac ctgggctgcg
     2401 taatagcgca ggaagtcgac cgcttcccgc acttcggcaa tcgcgttcgg cagcgacttg
     2461 ccggcttcgc gcaccgccag cgccatgaat tcctcgcgct gcgcttccag caggtcggcc
     2521 gcgcgttcca gcagcgccgc gcgctggccg gccggcgtgc tttcccagct ggccgcatag
     2581 gcggtggccg cggccagcgc ggtttccaca tcctgcgcat ccgcctcgac gacccggccg
     2641 accaggtcgc gcccgtccgc cgggttgcgc accgcctgcg cggtggtggc ggacaccgtt
     2701 ccggcgatca gcggtgcggc ggaccactgg tgcgtggaga aattccgaaa ggcatgcccg
     2761 agcgcctgca gcaccgactc gtttgccaga tccacgccca tcgaattgcg gcgctcggcg
     2821 ccgaacaggt cgaccggcag cgcaatgttc ggatgcggcg cgccgcccga ctggcgtgcg
     2881 ccttcgaacg gattggcgac cagctggtcg atgctcacgt tctcgtcgac gatctggttc
     2941 acgaacgagg agttcgcgcc gttttccagc aggcggcgca ccaggtaggc gagcagggtc
     3001 tcgtgcgagc cgaccggcgc atagatgcgg cagggcttgc caagcttgtc ggcgccgacc
     3061 acctggtcgt acagtgtttc ccccatgccg tgcaggcact ggaactcgta gtcgtccacg
     3121 cgctgctcct gcgcccaggt gtggatgctt gcaagcgtgt gcgcattgtg ggtggcgaac
     3181 tgcggataga tgacgtcgct cgccgccagc agcttgcgcg cgcaggcgag gtaggacacg
     3241 tcggtgtgga tcttgcgcgt atagaccggg tagcctggca tgccgtcgac ctgcgcgcgc
     3301 ttgatctccg catcccagta cgcgcccttg accagccgca ccatgaagcg gcggccgctg
     3361 cggcggccca gatcgaccag gtaatcgatc acgaacgggc agcgcttctg gtaggcctgc
     3421 acgacgaagc cgatgccgtc gaaaccggcc agatccgcat cgaaggccag cgcctccatc
     3481 aggtcgagcg acaactcgag gcggtcggct tcctcggcgt cgatgttcag gccggtgtcg
     3541 tactgcctgg ccagcagcag cagcgccttc aggcgaggca gcaattcggc catcacccgt
     3601 tcgcgctggg cgcgggaata gcgcggatgc aatgccgaca gcttgaccga gatgccgggg
     3661 ccgttgcgga tgccgcggcc gccggacgcg cggccgatcg cgtggatcgc atgctcgtag
     3721 gcggcgtagt actttgccgc atcctcggcc gtcagcgcgg cttcgccgag catgtcgtag
     3781 gagtagcggt agccgcgctt ttcattgtcg cggctgttct tcagcgcctc ctcgatcgtc
     3841 tgaccggtca cgaactgatt gcccagcatg cgcatcgcca gatcgacgcc cttgcggatc
     3901 aggggctcgc cgcccttggc gagcaggcgt gtgatcgccg aactcaggcc ctgttcgctg
     3961 ctggtcgaca ccagcttgcc ggtgatcagc agaccccagg tggccgcgtt gacgaacagc
     4021 gaaggcgatt cgccgaggtg acgcttccag tcgccgcggc tgatcttgtc ggcgatcagg
     4081 cggtcggcgg tttccttgtc cggaatgcgc agcagggctt cggccaggca catcagcgcg
     4141 acgccttctt ccgaggacag ggagaattca tgcatcagcg catcgacgcc ggaggcgcgc
     4201 gtgcgctttt cccgcaccgc ggcgaccagc ctgcgcgcga gcgcctcggc ctgcggccgg
     4261 gcctgggcat cgccgggcac ctgcgcgagc agccactgaa cggcttcggg ctcgtcacgg
     4321 cgccaggccg cggtgatggc ggcgcgcagc ggcgaagggg cctgcgcgat atcgtcgaaa
     4381 aaggcggaaa atgctggacg ggcagcacgt gtttcggtca acatggcgat cattggttgg
     4441 ggttgcgatg attcgattct agtgatgttt tgttcttatt aattctggaa atctcgctct
     4501 tccacggaat ttttcgcttc gtgggaaact atcaccgaag aaaatttatc tctccccgta
     4561 gcgcccatgc tagacaagat cagcaagaaa atcctctccg agctgcagaa cgacggccgg
     4621 atcagcaacg tggagctcgc ctcgcgcgtc aatctctcgc ctgccgcctg cctcgaacgc
     4681 gtgcgcaagc tgcaggaagc cggctacatc ctccactaca ccgcccagct caatccgcaa
     4741 ctgctcgacg tatcgctctt ggtgttcatc gaagtcgtgc tcgatcgcac cacgcccgaa
     4801 gtgttcgaag cgttcaagcg cagcgtgcag gtgattccgg aagtgcagga atgccatatg
     4861 gtggccggcg gcttcgacta cctcgtcaag gcgcgcgtga aggacatgaa cgcctaccgc
     4921 gaattcctcg gcaaatcgct gctgcagctc aaaggcgtgc gcgagacgca cacctatgcg
     4981 gtgatggaag aggtgaagaa cacaaccaag ctgccgatcc gctgaaggag cgacgatgct
     5041 ggaactgcga cccggttgcg aacattgcga cacgcctctc cctcctgagg ccaccaatgc
     5101 gatgatctgc agcttcgaat gcacgttctg cgcggactgc gtgagcaagg tgctgagcaa
     5161 tgtctgtccc aactgcgcag gcggcttctg gccgcggccg gtgcggccgc gcgtcaatcg
     5221 caagggcggg aacttcctgg gcaattatcc ggccggtacg cgcgtggtcc acaagcctgt
     5281 agacagcgag gcgcatgcgg agctcgtcgc ccggtgcgca tccatccctc cggcgcaaag
     5341 gtaagcgaca gcaggtattg cgcaagctca ccgggcttcc ggcattccat gctatcgtgc
     5401 aacgcacctg ctttgggcgc ggcggagtgg gtgtgccgcg catgccaccg aatcgacgag
     5461 cccgatgcgt atccgaaccc gcctgctgct gctggtgctg gccattctgc tgccagcgtc
     5521 cctgtgcgcg gtcgtcgcgc tcgcctatgc ctatcacgag acccaggact ttcagcgcca
     5581 ggcaatgcgc gagacggcgc gggcgctgtc tctgggcctg gaacgcgaac tggcgcggcg
     5641 cgagtcgatc ctgcatgcac tggccgcgtc gccggcgctg gataacgacg atttccgcaa
     5701 cttctatgcg cacgccaagc agattgccga cgccggcgac gtagcgattt ttctgtccac
     5761 ctccgacggc cggcagctgc tcaacacgcg cgtaccctac gggaagccgc tcccgcgcat
     5821 gctgccgcag gcgctcgaga tgcgcgccga agccggcccg cgcgccacgc tgatcagcaa
     5881 tctgtacgag ccgccggtcg gaaaaggcta cagcttcgcg atccagatac cggtcgagcg
     5941 caacggccgc gtgcgctatt acctgaacat ggggtcctat gccgcacagc tgcagtcgct
     6001 gctcgagctg caacggcttc cgcccgaatg gatagccggc attgccgatg cccacggcgt
     6061 gatcgtggca cgcaaccgcg acgccgaacg ctacgtcggc gtgtcggtac gccggacggt
     6121 tggccacctg ctcgaagccg gcgaaggctt tgccgaaggg cgggcgcttt ccggcgaggc
     6181 ggcgacggtg ttcctgagcc acgcgccgca ctatggctgg aatttctacg tggcggtgcc
     6241 gacggacctg ctccagcggg ctgcgtggcg caccaacgca tggctggcgc tggccatgct
     6301 cctgctgctc ggactcgcgt tgctggcggc gctcaagatg gcgcgcgaca cagcgggtgc
     6361 aatcgaagcc acgcggcgcg ccgcgcagcg tctcggagaa cgccggccgg tgcgtgtgca
     6421 gccaagcggg ctggtggagc tcgatacggt aaacgccgcg ttggccgagg ccggcaccag
     6481 cctccaggcc gcgcacgaag accaggagcg ccgcatcgcc gacgcggtcg ccagcgcgga
     6541 acgctcgcag cgcgcgctgc tgcatgcgca gaagctcgag gcgcttggac gcctgaccgg
     6601 cggcatcgcg catgacttca acaacatcct gcaaacggtc agcaccggcg tggaactgac
     6661 gcgccggctg actaccgatg cgcgtgcact cggcacgctc gacaactgcg cgcgcgcggt
     6721 gagacgggcg gcggagctga cgcgccagct agcggtattc ggacggcgcc aggattcgcg
     6781 cattgaaacc atcgatccgg tcaggcacct gacggaagtg cggccgctgc tcgcgggtgg
     6841 attgcgcagc gatatcgaac tgaacgtcga cattccgacc tcgctttggc acatcacggt
     6901 cgatccgctc cagttcgaac tggcgctgct gaacctgacg atgaatgcgc gcgacgcaat
     6961 gcccgccggg ggcaagctgc gcctgtgcgc gcacaatgaa accttgaccg tgccgctgga
     7021 cgagctgcag ccaggcgact acgtgcgcat ctcgctgctg gacaatggcg agggcatgac
     7081 gccggaagtg ctggcgcgcg cgatcgatcc cttcttcacc acgaagggag tcggccaggg
     7141 caccggcatg gggctgtcgc aggcatacgg cttcgcgcgt cagagcggcg gcaccctgct
     7201 cctgcagagc cagtcgggca agggaaccga cgccatcctc tatctgcctc gggcactgca
     7261 gatgccgatc acgcccgcgg tcgccggcag tggcggcagc aagcgccgcg ccgacggcga
     7321 acgcgtgctg ctcgtcgacg acgacgccca ggtgcgcgag gtcgtgcggg ccgcgctcga
     7381 gagccgcggc ttcagcgtgc tcgaagccga ggatggcgac gaagcgatac gcatgctgca
     7441 atcgggaatc gccgtcgatg cggtcctgtc cgacatcgtg atgccgggca gcctgtcggg
     7501 catcgatctg gcgcaccaac tgcatgccga gcatccgaat gtcgcggtac tgctgacgac
     7561 cggctacagc gagcgcagga tcgacctgcg cggtatccgg acccttccca agccttattc
     7621 gaccgatgat gcggccgaag ccctgctcga tgcgcttgcc gaggtacgct agctgtcacg
     7681 atcctgtcat gaagcggcgc tagcatccgg tgacctcaag caatcaccac cgtgcccgca
     7741 gcgggcgcgt tcgccatgtc cgccgacccc tccctccttt ccggcaagcg cgaagagctg
     7801 gcgctgctgc gcttcggccc gctcgagggc cgcgtactgc atgcgctgtg ggccttccat
     7861 gaagtgacgc tcgccgacct gcaccacctg cttggcgacg ccgcgctcac gcccaccgtt
     7921 ctgcgcgcca cactggaacg cctgcacctg aaacggctga tccgcctgcg caaggtgcat
     7981 cggacctgct tctaccgcgc cgcgctcgac cagtccacct tcatcagcct gctgcaccac
     8041 cagctcgccg gctttctcgg agaggaaggc atgtcgcagc tgcggcatct cggcgcgtca
     8101 gtttgaccgg cgctgccggc ctgtcatcaa accgtcattt ttccgtcatt aacgcgtcat
     8161 atctcatcgc taagctgcgc gcagtttctt ttcgtcacac gcttaggaga gcgcaaatga
     8221 acatgaggaa gtggattccc gccgcaggcc tggttgccgc agcaatgagc aatgtggtgt
     8281 tcgcccaggc cgtgcaggtc gatcccgcac tggccgacta tcagaaggcc gccggcgtct
     8341 ccggcaactt caccagcgtc ggttccgaca cgctgaacaa tctgatgacg ctgtgggctg
     8401 aagagttcaa gaaaacctat ccgaacgtga acatccagat tcagggtgca ggttcctcga
     8461 ccgcaccgcc ggccctgacc gaaggcgcat cgaacttcgg cccgatgagc cgcatgatga
     8521 atgcgaagga gatcgaaagc ttcgagcgcc gtcatggcta caagccgacc gctgtgccgg
     8581 tcgcgatcga tgcgctggcc gtgtacgtgc acaaggacaa cccgatcaag gggctgtcgc
     8641 tggaagacgt cgacgcgatg atgtcggtgg cacgcaagtg cggcagcgcg gccgacgtca
     8701 ccaagtgggg ccagatcggc atgtccggcg aatgggccaa ccgcgacgtg gcgatgtacg
     8761 gccgtaactc ggtctccggc acctacggct tcttcaagga caccgcactg tgcaagggcg
     8821 acttcaagcg caacgtcgcc gagcagccgg gttcggcttc ggtggtgcag agcgtgtcga
     8881 cccagatcaa tgcgatcggc tactcgggca tcggctacaa gacctccggc gtgcgcgcac
     8941 tgccgctgtc gaagaagcgt ggcgaggcgt ttgtcgaacc ggatgccgct cacgccgtcg
     9001 acggttccta tccgctcgcc cgcattctct acgtctacgt gaacaagaag ccgaaccagc
     9061 cgctggctcc gatcgagcgc gagttcgtca agctggtgct gtccaagcaa ggtcagcaag
     9121 tcgtggtgaa ggacggcttc gtgcccctgc ccgctcgtat cgccaaccag gcgctggaac
     9181 agctcgtcaa gtaatcgtgc agcacgcagg ctggcgttgc cggtctgcgt gcgtgacctg
     9241 acaggagaaa acgcatgaac aagaccatca agctggccgc gctggcatcc ggcatgtttg
     9301 ccgccctcgt tctggccggc tgtgcggccc ccacgcaaca ggcccaggcg ccggctgcgc
     9361 ccgcagctgc cgccgcccct gctgctccgg cagccgtcgc agcagcgcgc gaattctatg
     9421 tggtgctgcc ggaagacggc cgctactacg ccttcgccga tacgaagaac tacttcggct
     9481 tcctcgccca cgccgaagtt ccgctgacgc gcacccgcat cggtgccggc cccggcggca
     9541 agaccgtggt gttcggcatc agcggcgacg acgtcaagaa caaccgcccg agcatggccg
     9601 agcaggtgtt cgacggcaag ctgcaggctg acgccaactt ttatggcgaa gtgttcaaga
     9661 acggccgctt ctacgtgttc ggcaacatga aggacatgat ggacttcgcg cagttcggtg
     9721 aagtgcctta ttcctttacc gacatcggcg cgggccccaa gggcgagacg ctggtctggg
     9781 tgatgaacaa ggacagctat gcgaagggtc gtccggtgac gacgattgcg cagttccaga
     9841 aggtgaggac ggtgcagtaa ggctgaaatt caccgtgtac tgcttgatcg tccacggtaa
     9901 agcccctttc ccctttcagg gggatgagga cggggtttcg cagagcactg ctctgcgccg
     9961 tccgaatcgg agccgcttgc aagcggcgac gtggacaggg ttgggatggg ggtagggact
    10021 tcgagctagc gatctagcct gttcgggctc tgcaccctca ccctaaccct aaccctcccc
    10081 ctggaaggga gagggaacag tacagcgcga tttcagggcg ccatcacccc ggccaactac
    10141 aacatgaaca cactcacccg cagccaaacc gccgtcacga tggaccgtgc gatgcatcgc
    10201 aggctggtga ccgacaaggt cttcaaggtc ctgatgtccg caggcggcat cggggtgatc
    10261 gtcgcggtgt ccgcgatctt cttctatctc gccagcgtcg tcgtgcccct gttcgttccg
    10321 ccttcggtcg aggcccaggc gagcttcgcg gcgccgggca gcgcggtcca gcgcaccggt
    10381 gcttactcgg gtgaggaaca gcgcgagatc ggcgtgcgca tcggcgcaac cggcgatgtc
    10441 agcttcttcg ggttcaagga tggtcgcgtg ctgcaggaaa gccgcatcac catgccggcg
    10501 ggcgcgcagc tcaccagctt cgcgctggcc gaccgcagca gctacgcgtt tgcctacggc
    10561 ctctcggacg gccgtgcagt agttgcgaaa caggcttaca aggtcacctt ccccgacaac
    10621 aagcgcctga tcacgcccgg catcgaatac ccgctcggcg aggacgccgt cgtgatcgat
    10681 ccgcgcgggt cagccctgca gcagctcgcg gtccagcacg gcgacagcgg caccacgctg
    10741 gccggctata ccgccgacgg gcgcttgctg gtttccgccg tgactgtgac caccaatttg
    10801 atgaccggtg aatcgcgcag cgaagcggcc caggccgagg tgccgggcgc gcccggcacc
    10861 tcggcctggg ccgcttcgct gcgcgattca
//
LOCUS       DATLGF010000218         3898 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_100402, whole genome shotgun sequence.
ACCESSION   DATLGF010000218 DATLGF010000000
VERSION     DATLGF010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100402"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VM406_06595"
     CDS             <1..251
                     /locus_tag="VM406_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HVL75666.1"
                     /translation="DAYLQQTWHASTRPQGELANATTKWLGLEVRRHQQDGDAAIVEF
                     IARYKVGGRAHRLHEVSRFVREEGNWYYVDGTIPERQQ"
     gene            248..1855
                     /locus_tag="VM406_06600"
     CDS             248..1855
                     /locus_tag="VM406_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HVL75667.1"
                     /translation="MTILESKLNVRSDDFKGNAAAMQALVDDLKAKVEKIALGGGEDA
                     RKKHTSRGKLLPRDRVQMLLDPGTPFLEFSQMAAYGMYQDAAPSAGIITGIGRVAGQE
                     CVIVCNDATVKGGTYYPMTVKKHLRAQEIADQNNLPCIYLVDSGGANLPNQDDVFPDR
                     DHFGRIFFNQSNMSAKGIPQIAVVMGSCTAGGAYVPAMSDESIIVKEQGTIFLGGPPL
                     VKAATGEVVSAEDLGGGDVHTRLSGVVDHLAQNDMHALALARKITGNLNRRKPQQLVL
                     REPVAPKYDPRELYGVIPTDTRKPFDVREVIARIVDGSEFDEFKARYGTTLVCGFAHI
                     FGMPVGIIANNGILFSESALKGAHFIELCCQRKIPLVFLQNITGFMVGRKYENEGIAR
                     NGAKMVTAVSTASVPKFTVIIGGSFGAGNYGMCGRAYSPRFLWMWPNARISVMGGEQA
                     ASVLATVKRDGIEAKGGSWSKEEEEAFKQPIRDQYEHQGHPYYATARLWDDGVIDPAD
                     TRMVLGLGLSASLNAPIPDTKFGVFRM"
     gene            1858..2643
                     /locus_tag="VM406_06605"
     CDS             1858..2643
                     /locus_tag="VM406_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HVL75668.1"
                     /translation="MDYQTLLLAIEDRVATVTLNRPDVRNAFNETTIAEITHVFRALG
                     ENAEVRAIVLAANGPAFCAGADLNWMKKMAGYSHDENRADAAQLAAMLRTIYTCPKPV
                     LAKVQGDCYAGGMGLVAACDIAVATDAVNFCLSEVKLGLIPATISPYVIKAMGENASR
                     RYFLTAERFSAQEAQRIGFVHQVVGVDALDTVCGEIVKALVNNSPNAVKEAKRLVQDV
                     AGRELSDALIADTAERIADIRSSEEGKEGVKSFLEKRKPNWLG"
     gene            2717..>3898
                     /gene="bioA"
                     /locus_tag="VM406_06610"
     CDS             2717..>3898
                     /gene="bioA"
                     /locus_tag="VM406_06610"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HVL75669.1"
                     /translation="MNPTPQPELVARSLSSVWHPCTQMQHHETMPLVPLSHGKGAWLY
                     DPQGRRYLDAISSWWVNLFGHANQHINDALKDQLDKLEHAMLAGFTHEPVVALSERLA
                     ERTGNVLGHCFYASDGAAAVEIALKMSFHFWRNSGKADKQEFVCLKDSYHGETVGALR
                     VTDVPLFRDAYGPMVQAAHVIASPDGRKARDGESGVDVALNAAGELEALLQRRADKIA
                     AIIVEPLVQCAAGMAMYDPVYLSEVRRLCDQYGVHLIADEIAVGCGRTGTFFACEQAG
                     IWPDFLCLSKGISGGYLPLSLVMTRDAIYRAFYDADVKRGFLHSHSYTGNPLACRAAL
                     ATLSIFDHDNVLENNRARAQRLTESLLPLAVDARVNHFRQRGMIWAFDVDTPDPAFAR
                     RF"
BASE COUNT          731 a         1213 c         1297 g          657 t
ORIGIN      
        1 aggacgcgta tctgcagcag acctggcatg cgagcacccg cccgcaaggc gagctggcga
       61 atgcaaccac caaatggctg ggcttggaag tacgccgcca ccagcaggac ggcgatgcgg
      121 cgatcgtgga atttatcgcc cgctacaagg tcggcgggcg tgcgcaccgg ctgcatgaag
      181 tgagccgctt cgtgcgtgaa gaaggtaact ggtactacgt ggacgggact atcccggaaa
      241 gacaacaatg acaattcttg aatcgaaact caatgtccgt tcggacgact tcaagggcaa
      301 tgcggctgcg atgcaggcgc tggtggacga cctgaaggcg aaggtggaaa agatcgcgct
      361 gggcggcggc gaggatgcgc gcaagaagca cacgtcgcgc ggcaagctgc tgccgcgcga
      421 ccgcgtgcag atgctgcttg acccgggcac gcccttcctc gagttctcgc agatggcggc
      481 ttacggcatg tatcaggatg cagcaccgag cgccggcatc atcaccggga tcggccgcgt
      541 ggccggccag gagtgcgtga tcgtctgcaa cgacgcgacc gtcaagggcg gcacctatta
      601 cccgatgacg gtgaagaagc atttgcgcgc gcaggagatc gccgaccaga acaatctccc
      661 ctgcatctat ctggtcgact ccggcggcgc caacctgccg aatcaggacg acgtcttccc
      721 ggaccgcgac cacttcggcc gcatcttctt caaccagtcg aatatgtcgg ctaagggcat
      781 cccgcagatc gcggtggtga tgggttcctg cacggccggt ggcgcctacg tgccggcgat
      841 gagcgacgaa tcgatcatcg tgaaggaaca gggcacgatc ttcctcggcg gaccgccgct
      901 ggtgaaggcg gcgaccggcg aagtcgtcag cgccgaggat ctcggcggcg gcgatgtgca
      961 cacgcgtctg tccggcgtgg tcgatcacct cgcgcagaac gacatgcatg cgctggcgct
     1021 ggcgcgcaag atcaccggca acctcaatcg caggaagccg cagcaactgg tgctgcgcga
     1081 gcctgtcgcg ccgaagtacg atccccgcga gctgtacggc gtgatcccga ctgacacgcg
     1141 caagcccttc gatgtgcgcg aggtgatcgc gcgcattgtc gacggcagcg agttcgacga
     1201 attcaaggcg cgctacggca ccacgctggt gtgcggcttc gcccacatct tcggcatgcc
     1261 ggtcggcatc atcgccaaca acggcatcct gttctccgag tccgccttga agggcgcgca
     1321 cttcatcgag ctgtgctgcc agcgcaagat cccgctggtg ttcctgcaga acatcaccgg
     1381 cttcatggtc gggcgcaagt acgagaacga gggcatcgcg cgcaacggcg cgaagatggt
     1441 gaccgcggtg tcgaccgcgt cggtgccgaa gttcaccgtg atcatcggcg gcagcttcgg
     1501 cgccggcaac tacggcatgt gcggccgagc gtattcgccg cgcttcctgt ggatgtggcc
     1561 gaacgcgcgc atctcggtga tgggcggcga gcaggcggcg tcggtgctgg cgaccgtcaa
     1621 gcgcgacggc atcgaagcaa agggcggcag ctggagcaag gaagaggaag aggcgttcaa
     1681 gcagccgatc cgcgaccagt acgaacacca gggccatccg tattacgcca ccgcgcgcct
     1741 gtgggacgac ggcgtgatcg atccggccga cacccgcatg gtgctgggcc tgggcctgtc
     1801 ggcctcgctg aatgcaccga ttccggacac gaagttcggc gttttcagga tgtgatgatg
     1861 gactaccaga ctttattgct tgcaatcgag gaccgcgttg ccacggtcac gctgaatcgg
     1921 cccgacgtgc gcaatgcgtt caatgaaacg acgattgccg aaatcaccca tgtcttccgt
     1981 gcgctcgggg aaaacgccga ggtgcgcgcg atcgtgctgg ccgccaacgg ccccgcgttc
     2041 tgcgccgggg cggacctgaa ctggatgaag aagatggccg gctattcgca cgacgaaaac
     2101 cgtgccgacg ccgcccagct cgccgcgatg ctgcgcacga tctatacctg cccgaagccg
     2161 gtgctggcaa aagtgcaagg cgactgctat gcgggcggga tggggttggt cgctgcctgc
     2221 gacatcgcag tggcgaccga cgcggtcaat ttctgcctgt ccgaggtcaa actcggcctc
     2281 attcctgcga caatctcgcc ttacgtcatc aaggccatgg gcgagaacgc gtcgcgccgc
     2341 tatttcctga cggccgagcg cttttccgcg caggaagcgc agcgcatcgg cttcgtgcat
     2401 caggtggtcg gcgtcgacgc gctggacacc gtgtgtggcg aaatcgtcaa ggcgctggtg
     2461 aacaacagcc cgaacgcggt gaaggaagcg aagcggcttg tgcaggacgt ggcggggcgg
     2521 gagctgagtg atgccttgat tgccgatacc gccgagcgga ttgcggatat ccgcagttcg
     2581 gaagagggca aggagggggt gaagtcgttt ctggagaagc gcaagccgaa ttggttaggc
     2641 tgagctttgc tcccctctcc cgttccggga gaggggagca cgaagattga agcttgaaag
     2701 aatttttgga gcaggtttga accccacccc acaacccgaa ctcgtcgcac gcagcctttc
     2761 cagcgtctgg cacccgtgca cgcagatgca gcatcacgag acgatgccgc tggtgccgct
     2821 gtcgcacggc aagggcgcat ggctgtacga cccgcaaggg cgtcgctacc tggacgcgat
     2881 cagctcgtgg tgggtgaacc tgttcgggca cgcgaaccag cacatcaacg atgcgctgaa
     2941 ggatcagctc gacaagctcg agcatgcgat gctggccggc ttcacgcatg agccggtcgt
     3001 ggcattgtcc gaacggctgg ccgagcgcac cggcaacgtg ctcggccact gcttctatgc
     3061 gtccgacggc gccgccgcgg tcgagatcgc gctgaagatg agctttcatt tctggcgcaa
     3121 cagcggcaag gccgacaagc aggaattcgt ctgcctgaag gacagctatc acggagaaac
     3181 cgtgggcgcg ctgcgcgtca ccgatgtgcc gctgttccgc gacgcctacg gcccgatggt
     3241 gcaggccgcg cacgtgatcg cctcgccgga cgggcgcaag gcgcgtgacg gggagtcggg
     3301 cgtcgacgtg gccctgaatg ccgccggcga gctcgaggcg ctgctgcagc ggcgcgcgga
     3361 caagatcgcg gccatcatcg tcgaaccgct ggtgcagtgc gcggccggca tggcgatgta
     3421 cgacccggtc tacctgtccg aagtgcgccg gctgtgcgac cagtacggcg tgcacctgat
     3481 cgccgacgaa atcgcggtcg gctgcgggcg caccggcacc ttcttcgcct gcgaacaagc
     3541 gggcatctgg ccggatttcc tctgcctgtc caagggcatt tccggcggct acctgccgct
     3601 gtcgctggtg atgacacgcg acgcgatcta ccgggccttc tacgacgccg acgtcaagcg
     3661 cggcttcctg cattcgcatt cgtatacggg caatccgctg gcctgccgcg cggcgctggc
     3721 gacgctttcg atcttcgacc acgacaacgt gctcgaaaac aaccgcgccc gcgcgcagcg
     3781 gctgaccgaa tcgctgctgc cgctggccgt cgatgcgcgc gtgaaccatt tccgccagcg
     3841 cggcatgatc tgggcctttg atgtcgatac gccggacccg gcattcgcgc gccgtttc
//
LOCUS       DATLGF010000219         3927 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_87854,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000219 DATLGF010000000
VERSION     DATLGF010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87854"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VM406_06615"
     CDS             <1..391
                     /locus_tag="VM406_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75670.1"
                     /translation="RVADIMTEISAASEEQSAGIEEVNRAITQMDEMTQQNAALVEEA
                     AAAAQSMQDQATRLAEAVDAFKIAYAAASVQPAARPAPVMQAAGPQVTAPARVAAKLP
                     ASSARAPKAVAPARRGKPAAGDDWEEF"
     gene            593..1411
                     /locus_tag="VM406_06620"
     CDS             593..1411
                     /locus_tag="VM406_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HVL75671.1"
                     /translation="MRALIYRRAGIALADSKQEMVYSRLARRLRAIGMRSFESYLDRL
                     EHDPGSSEWEAFTNALTTNLTSFFREAHHFPILAEHVRRRGEPVSIWCAASSTGEEPY
                     SIAMTLCETLGAKAAACQVIATDIDTNVLATAAQGIYPIERVEKMDSERVRRFFLRGK
                     GAHAGQVRVRPELREMVTFRHLNLLAPDWPVSGPFDAIFCRNVMIYFDKPTQGRILTR
                     FVPLMKPDALLFAGHSENFLYVSDAFKLRGKTVYELDSKRHPLQHSAAALKSTP"
     gene            1408..2019
                     /gene="cheD"
                     /locus_tag="VM406_06625"
     CDS             1408..2019
                     /gene="cheD"
                     /locus_tag="VM406_06625"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase CheD"
                     /protein_id="HVL75672.1"
                     /translation="MSYGIEEQFATNVYYDRTFDCDAAKILPGEYYYTCKDMMIVTVL
                     GSCVSACIRDRVTGIGGMNHFMLPDGGGDADSPVSASMRYGTYAMEVLINDLLKAGAR
                     RENLEAKVFGGGNVLRGFVAINVGERNAQFVRNYLRAENIRVLAEDLNDIYPRKVYFF
                     PRTGKVLVKKLRQLNNNTLVNREQDYAQRLQTVPVAGDIELFS"
     gene            2043..3119
                     /locus_tag="VM406_06630"
     CDS             2043..3119
                     /locus_tag="VM406_06630"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HVL75673.1"
                     /translation="MKIKVLIVDDSALIRSVMNEIIRKQPDMEVVGVAPDPIVARELI
                     KKTNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTERGSEITLRALELGAVD
                     FVTKPKLSIQSGMLEYAELITDKIRAAAKARVRARTVASSEAGKAASEGLPLIRNPLT
                     SSEKLIIIGASTGGTEAIKEFLVQMPSDCPGILITQHMPEGFTRSFAKRLDTLCKITV
                     REAEGGERVLPGHAFIAPGHSHLQLVRSGANYMTQLDQGPPVNRHRPSVDVLFESAAR
                     CAGKNAVGVILTGMGKDGAAGMLRMREAGAYNFAQDEASCVVFGMPREAIAMGAAHEV
                     GALTALPGMVLNHLATHSSRALRV"
     gene            3230..3619
                     /gene="cheY"
                     /locus_tag="VM406_06635"
     CDS             3230..3619
                     /gene="cheY"
                     /locus_tag="VM406_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HVL75674.1"
                     /translation="MAGPNTKFLVVDDFSTMRRIVRNLLKELGYTNVDEAEDGAMALS
                     KLRSEQFDFVISDWNMPVMDGLTMLQQIRADPALSKMPVLMVTAEAKKENIIAAAQAG
                     ANGYVVKPFTAATLDEKLSKIFEKLGI"
     gene            3622..>3927
                     /locus_tag="VM406_06640"
     CDS             3622..>3927
                     /locus_tag="VM406_06640"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HVL75675.1"
                     /translation="MDQAAVRPGAAVQGVAQEEVLARVGQMTRMLHENLRGLGFDKLI
                     AKAAHDMPDARDRLDYVAKMTEQAAQRVLNATEAAAPLQERIESGAGDLAGEWRALMD
                     "
BASE COUNT          760 a         1235 c         1304 g          628 t
ORIGIN      
        1 gcgcgtcgcc gacatcatga ccgagatctc ggcggccagc gaggagcaga gcgcgggcat
       61 cgaggaagtc aaccgcgcga tcacccagat ggacgagatg acgcagcaga acgcggcgct
      121 ggtcgaggag gccgcggccg ccgcccagag catgcaggac caggccacgc ggcttgccga
      181 agcggtcgat gcgttcaaga tcgcctatgc cgcggcgtcc gtccagccgg ccgcccgacc
      241 ggcgccagtg atgcaagcgg cgggcccgca ggtcacggcg ccagcacggg tcgcggcgaa
      301 gctgcctgcc tcgtcggcgc gcgcgccgaa ggccgtcgca ccggcgcgcc ggggaaaacc
      361 ggctgcgggc gacgactggg aagagttctg acccgacgac tgatacgcac agatttgcga
      421 cattgagccg gcattgcgcc ggcggagaaa tgggatgagg aacgatgtcg tgaatgacgc
      481 agctggcgcg tcggcgcgga ctgcccgcgc gcccgcgaag gcgacttccg gggggagcgg
      541 cgggtccgac gcgctcaagg aattcgcttt cacaccgcgc gactttgagc gcgtgcgtgc
      601 gctgatctac cgccgtgccg gcatcgcgct ggccgacagc aaacaggaaa tggtatacag
      661 ccgtctcgcg cgccgcctgc gcgcaatcgg catgcgctcg ttcgaatcct acctggaccg
      721 gctcgagcac gaccccggca gctccgagtg ggaggcattc accaatgccc ttaccaccaa
      781 cctgacttcg ttctttcgcg aggcgcatca ctttcctatc ctcgccgagc atgtgcgaag
      841 gcgcggcgag ccggtcagca tctggtgcgc ggcgagctcc accggagagg agccgtattc
      901 gatcgcaatg accttgtgcg agacgctagg agcgaaggcg gccgcctgcc aggtgatcgc
      961 gactgacatc gacaccaatg tgctggcgac ggccgcacag gggatctacc cgattgaacg
     1021 ggtcgagaaa atggatagcg agcgtgtgcg ccggtttttc ctgcgcggca agggggcgca
     1081 tgcgggccag gtgcgcgtgc ggccggaact gcgcgagatg gtcacattcc ggcatctcaa
     1141 cctgctggcg ccggactggc cggtctcagg gccgttcgac gcgatcttct gccgcaacgt
     1201 gatgatctat ttcgacaagc cgacgcaggg tagaatcctg acgcgctttg ttccattaat
     1261 gaaaccggac gccctgctgt tcgccggcca ctcggaaaac ttcctgtacg tgtccgatgc
     1321 gttcaagttg cgcggcaaga ccgtctacga actggattcg aagcgtcatc cgctgcagca
     1381 ttcggccgca gccttgaaga gcacgccatg agctacggaa tcgaagagca gttcgccacc
     1441 aacgtctatt acgaccgcac cttcgattgc gatgcggcga agatcctgcc gggcgagtac
     1501 tactacacct gcaaggacat gatgatcgtg actgtgctcg ggtcctgcgt gtcggcctgc
     1561 atccgtgacc gcgttaccgg catcggcggc atgaaccact tcatgctgcc cgacgggggc
     1621 ggcgacgccg acagccctgt ctcggcctcg atgcgctacg gcacctacgc gatggaagtg
     1681 ctgatcaacg acttgctcaa ggccggagcg cggcgcgaga acctcgaggc caaggtgttc
     1741 ggcggcggca acgtgctgcg cggctttgtc gcgatcaacg tcggcgagcg caatgcccag
     1801 ttcgtgcgca actacctgcg cgccgagaac atccgggtgc tcgccgaaga tctgaatgac
     1861 atctatccgc gcaaagtgta cttctttccg cgcaccggca aggtgctggt gaagaagctg
     1921 cgtcagctca acaacaacac gctcgtcaat cgcgaacagg attacgcgca gcgtctgcag
     1981 accgtgccgg tggcgggcga catcgaactg ttcagttagc atcgcacggg aacaaccgca
     2041 tcatgaaaat caaggtcctg atcgtcgacg actccgcact gatccgcagt gtgatgaacg
     2101 aaatcatccg caagcagccc gacatggagg tggtcggcgt cgcccccgat ccgatcgtgg
     2161 cgcgcgagct gatcaagaag accaaccccg acgtgctgac gctggacgtc gaaatgccgc
     2221 gcatggacgg cctcgatttt ctcgaaaagc tgatgcggct gcgcccgatg ccggtcgtga
     2281 tggtctcgtc gctgaccgag cgcggctcgg aaatcacgct gcgcgcgctc gaactcggcg
     2341 cggttgattt cgtcaccaag cccaagctgt ccatccagag cggcatgctg gaatatgccg
     2401 aactgatcac cgacaagatc cgtgcggcgg cgaaggcgcg cgtgcgcgcg cgcacagtcg
     2461 cgtccagcga ggcaggcaag gctgcgtccg aaggactgcc gctgatccgc aatccgctca
     2521 ccagtagcga gaagctgatc atcatcggcg cttccaccgg aggcacggag gcgatcaagg
     2581 aattcctggt ccagatgccg tcggactgcc ccggcatcct gatcacccag cacatgccgg
     2641 aaggttttac gcgctcgttc gccaagcgcc tcgacacgct gtgcaagatc acggtgcgcg
     2701 aggccgaggg tggcgaacgg gtgctgccgg gccatgcgtt catcgcgccg ggccactcgc
     2761 acctgcagct tgtgcgcagc ggcgccaact acatgaccca gctcgaccag gggccgccgg
     2821 tcaaccgcca ccgtccgtcg gtcgacgtgc tgttcgagtc cgccgcgcgc tgcgccggaa
     2881 agaacgcggt tggggtgatc ctgaccggaa tgggcaagga cggcgcggcc ggcatgctgc
     2941 gcatgcgaga ggccggcgcc tacaacttcg cgcaggacga ggccagttgc gtggtgttcg
     3001 gcatgccgcg cgaagccata gcaatgggcg cggcccacga ggtcggcgct ctgaccgcat
     3061 tgcccgggat ggtgctgaac catctggcaa cccacagcag ccgggcgttg cgggtgtagt
     3121 acgtttggcg ctgcacaacg ggaaggcgag cgtttatact tagggccgat agtgccgtac
     3181 ggcaatatcc gggcagttca ctacgcagta cagatttgac ggagcatcca tggcgggtcc
     3241 caatacgaaa ttcctggtcg tcgacgactt ttccacgatg cgccgcatcg tccgcaacct
     3301 gctcaaggag ctgggctaca cgaacgtcga cgaagcggag gacggcgcga tggcgctgtc
     3361 caagctgcgc agcgaacagt tcgatttcgt gatctccgac tggaacatgc cggtgatgga
     3421 cggattgacg atgctgcagc agatccgcgc cgatcccgcg ctgtccaaga tgccggtgct
     3481 gatggtgacc gcggaagcga agaaggaaaa catcatcgcc gccgcgcagg caggggccaa
     3541 cggctacgtg gtcaagccgt tcaccgcggc cacgctggac gaaaagctgt cgaagatctt
     3601 cgagaaactc gggatctgac ggtggaccag gcagcggtgc ggccgggcgc ggcggtgcag
     3661 ggcgtcgcgc aggaagaggt gctggcgcgc gtcggacaga tgacgcgcat gctgcatgag
     3721 aacctgcgcg gtctcggctt cgacaagctg atcgccaagg cggcgcatga tatgcccgac
     3781 gcgcgcgacc gtctcgacta cgtcgcgaag atgacggagc aggccgccca gcgcgtgttg
     3841 aacgccaccg aagcggccgc tccgttgcag gagcgcatcg agtccggcgc cggcgacctc
     3901 gccggcgaat ggcgcgcgct gatggac
//
LOCUS       DATLGF010000220         4466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_25492,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000220 DATLGF010000000
VERSION     DATLGF010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25492"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..706
                     /locus_tag="VM406_06645"
     CDS             113..706
                     /locus_tag="VM406_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HVL75676.1"
                     /translation="MEPQWKLIGALALCMGLAACGGGGEDSPDPVPTTPPPATGTPAP
                     GASTQLTSANTCGIADYQQSMLEQINAARATARLCGTRSFSAAPPLAWNDLLFAAAAG
                     HAEDMAVNNYFSHDALNGRTFSQRISDAGYNWSAAGENLAAGQAGISQVITAWLDSPG
                     HCENLMSTSFTEVGVACVRNDAARYGQYWAMSLGRPR"
     gene            862..2907
                     /locus_tag="VM406_06650"
     CDS             862..2907
                     /locus_tag="VM406_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HVL75677.1"
                     /translation="MSHSTHGMNPPQREAVNYMDGPCLVLAGAGSGKTRVITTKIAQL
                     IEQHGYDARNIAAVTFTNKAALEMQERIGKLLKEPKEAKNLTVCTFHSLGMQILRREA
                     RELGLKDRFSIMDSDDCFSIVQDLACTTDKQIVRRIQNTMSLWKNALVDPERALKEAT
                     TEDEGQAARIYRNYVATLAAYQAVDFDDLIRLPVELFQQNEAVRDKWQRKLRYLLVDE
                     YQDTNTCQYELVKLLVTGVGKKPMFTAVGDDDQAIYAWRGATIENLKQLQVDFPELKI
                     IKLEQNYRSSTRILQAANAVIGNNPKLFEKQLWSEHGLGDPVKVLAMQDDEQEAEQVA
                     IMISAHKFERRAKFSDYAILYRGNHQARVIEQALRKERIPYTISGGQSFFDRAEIKDI
                     VSYLRLIANQDDDPAFIRAVTTPKRGVGQSTLEALGAFAGQWQCSLFEAVFKGGIESK
                     LNDRQLYPLREFCNFINGLEARASKRGENAAELLDEMMKEINYEAWLYSAFDDRQAQS
                     KWQNVLEFTTWLKEKGTGGRDGTDPEKSLLELTQMVALMTMLEGRDEEPDAVRMSTLH
                     ASKGLEFPHVFLVGVEEGILPHKGDPDAPVETLAARIAEERRLMYVGITRAQRSLHIT
                     WCKRRKRAREYVNCDVSRFIKEMKLDEGDAVPKEEEVLSPKDRLANLKALLQKPRPA"
     gene            2961..3719
                     /locus_tag="VM406_06655"
     CDS             2961..3719
                     /locus_tag="VM406_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75678.1"
                     /translation="MAATLFARRFVFLVFLLAALQLSGCAGVQRGWDAALLLADLQAA
                     GGDSMLKRRTPAPHVTPLQYTIAGHAQVADRYAGPDASRGTLVLIHGFTEAGRRDVRM
                     VQFAESLARSGFVVLAPELPGMTDMSVGSAEREEIATALRHATGHGRAGLVALSLAAG
                     PAIHAAMKPDLADRVAYILLIGGYHDIVDVIRYATTGVDAATGETADLREPVQQGKWM
                     LLRAQLRNLEDPQDHAALEAIANARTQDPAAPVD"
     gene            3822..4088
                     /locus_tag="VM406_06660"
     CDS             3822..4088
                     /locus_tag="VM406_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75679.1"
                     /translation="MREELAALDLARHDLGALRAQLLLVHGAADPVIPLSQSKRLAGA
                     LPEGQVELFIPPDLSHVDLDPGLRDAWHLWRATTRLLAHAEASR"
     gene            complement(4095..4355)
                     /locus_tag="VM406_06665"
     CDS             complement(4095..4355)
                     /locus_tag="VM406_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75680.1"
                     /translation="MRTTKASAAVTRLNARDPEYRYSMGMTASGFLYLLRAKEGQPAE
                     RVSDDLTMEEFIELANRTGPQKKVKVSKLDQAFEKQLARKNT"
BASE COUNT          828 a         1477 c         1443 g          718 t
ORIGIN      
        1 gtatccggcc aagcgctttt tcacgccggc gtttgcgcaa tcttccggcg caggaaccaa
       61 gtcggcgctg tcccggtctg caatgagttc cagacaagaa aggagaggcg ccgtggagcc
      121 gcaatggaag ttaatcgggg cgctggcctt gtgcatggga ctggccgcct gcggtggcgg
      181 cggcgaagac tcgccggacc cggtcccgac aacgccgccc ccggccaccg gcacgccggc
      241 gccgggcgct tccacgcagc tgaccagcgc gaacacctgc ggcattgccg actatcagca
      301 gtcgatgctg gagcagatca atgccgcgcg cgcgactgcc cgcctgtgcg ggacgcgctc
      361 gttctcggcc gcgccgccgc tggcatggaa cgacctgctg ttcgccgcag ccgccggcca
      421 tgccgaagac atggccgtca acaactactt ctcccacgac gcgctgaacg ggcgcacctt
      481 cagccagcgt atcagcgatg ccggctacaa ctggtccgcc gccggagaaa acctcgccgc
      541 cgggcaggcc ggcatctccc aggtgatcac cgcctggctc gacagcccgg gacactgcga
      601 aaacctgatg agcaccagct tcaccgaggt cggcgtggcc tgcgtgcgca acgatgccgc
      661 gcgctacggc cagtactggg cgatgtcgct ggggcgcccg cgctaggatc agcgccctgt
      721 gcggcaaggg caggccgcgc ctgttctccc accttgacgt tgtgtcttgc cgttcttgca
      781 tgtcgcagac ctgtcacaac cggggccatc ctgcgctgct acaatggccc gctttccccg
      841 cagcaccgct cccgcatccg catgtcccat tccacccacg gcatgaatcc gccgcagcgc
      901 gaagccgtca actacatgga tggaccctgt ctggtcctgg ccggcgcagg ttccggcaag
      961 acccgcgtga tcaccaccaa gatcgcgcag ctgatcgaac agcacggcta tgacgcgcgc
     1021 aacatcgccg cggtgacctt caccaacaag gccgcgctgg agatgcagga acgcatcggc
     1081 aagctcttga aggagccgaa ggaagcgaag aacctgaccg tctgcacctt ccattcgctc
     1141 ggcatgcaga tcctgcgccg cgaagccagg gagctcggcc tgaaggaccg cttctcgatc
     1201 atggacagcg acgactgctt ctcgatcgtc caggaccttg cctgcacgac cgacaagcag
     1261 atcgtgcgcc gcatccagaa cacgatgtcg ctgtggaaga acgcgctggt cgatcccgag
     1321 cgcgcactga aggaggcgac caccgaggac gaaggacagg ccgcgcgcat ctaccgcaat
     1381 tacgtcgcca cgctggcggc ttaccaggcg gtggacttcg acgacctgat ccgcctgccg
     1441 gtggagctgt tccagcagaa cgaagcggtg cgtgacaaat ggcagcgcaa gctgcgctac
     1501 ctgctggtcg acgaatacca ggatacgaat acctgccagt acgagctggt caagctgctg
     1561 gtgaccggtg tcggcaagaa gccgatgttt accgcggtgg gcgacgacga ccaggcgatc
     1621 tacgcatggc gcggcgctac catcgaaaac ctcaagcagc tgcaggtcga tttccccgag
     1681 ctgaagatca tcaagctcga gcagaactac cgctcgtcca cgcgcatcct gcaggccgcg
     1741 aacgcggtca tcggcaacaa cccgaagctg ttcgagaagc agctgtggtc cgagcacggc
     1801 ctgggcgacc cggtgaaggt gctggcgatg caggacgacg agcaggaagc cgagcaggtc
     1861 gcgatcatga tctccgcgca caagttcgag cggcgcgcga agttctccga ttacgcgatc
     1921 ctgtaccgcg gcaaccacca ggcgcgcgtg atcgagcagg cgctgcgcaa ggaacgcatt
     1981 ccgtacacga tctccggcgg ccagagcttc ttcgaccgcg ccgagatcaa ggacatcgtc
     2041 agctacctgc gcctgatcgc gaaccaggac gacgatccgg ccttcatccg cgcggtcacc
     2101 acgcccaagc gcggggtcgg ccagtcgacg ctggaagcgc tcggcgcgtt cgccgggcaa
     2161 tggcagtgct cgctgttcga ggccgtgttc aagggcggca tcgaatccaa gcttaacgac
     2221 cgccagctct atccgctgcg cgaattctgc aacttcatca acgggctcga ggcgcgtgcc
     2281 tccaagcgcg gcgagaacgc cgccgaactg ctcgacgaga tgatgaagga gatcaactac
     2341 gaagcctggc tgtactccgc cttcgacgat cgccaggcgc aatcgaagtg gcagaacgtg
     2401 ctcgagttca cgacctggct gaaggaaaag ggcaccggcg ggcgcgacgg caccgatccc
     2461 gagaagagcc tgctcgaact cacgcagatg gtcgcactga tgacgatgct cgaaggccgc
     2521 gacgaagagc cggacgcggt acgcatgtcc acgctgcatg cgtcgaaggg gctggagttt
     2581 ccgcacgtgt tcctggtcgg cgtggaagag ggcatcctgc cgcacaaggg tgacccggat
     2641 gcgccggtag aaacgctggc cgcacgcatc gcggaagagc gccgcctgat gtacgtgggc
     2701 atcacccgcg cgcagcgtag cctgcacatc acctggtgca agcggcgcaa gcgcgcgcgc
     2761 gaatatgtga actgcgacgt ctcgcgcttc atcaaggaaa tgaagctcga cgagggtgac
     2821 gccgtaccga aggaggaaga ggtgctcagt cccaaggacc ggctggccaa cctgaaggcg
     2881 ctgttgcaga agcccagacc ggcctgaacc gatttccccc gtctccttcc aacggatagt
     2941 tcattggacg ggggtcagcc atggccgcca cgctgtttgc gcgccgattc gtttttctgg
     3001 tgtttcttct tgccgcgctg cagttgtcgg gttgcgccgg cgtgcagcgc ggttgggacg
     3061 cggcgctgct gctggccgac ctgcaggcgg ccggcggcga cagcatgctc aagcggcgca
     3121 cccccgcgcc gcacgtcacg ccgctgcaat acacgatcgc cgggcatgca caggtcgccg
     3181 accgctatgc cggacccgac gcgtcgcgcg ggaccctggt actgatccac gggtttaccg
     3241 aagcaggacg acgcgatgtg cgcatggtgc aattcgccga gagtctcgcg cgcagcggct
     3301 tcgtcgtgct ggcgccggag ttgccaggca tgaccgacat gtcggtcggc agcgccgagc
     3361 gcgaggagat tgcaacggcc ttgcgccatg cgaccgggca cggccgcgcc ggactggtgg
     3421 ccctgagcct ggccgccggg ccggccattc atgcggcgat gaagccggac ctggctgacc
     3481 gcgttgccta tatcctgctg ataggcgggt atcacgacat agtcgacgtg atccgctacg
     3541 cgaccaccgg cgtcgatgcc gcgaccggcg agacggccga ccttcgggaa ccggtgcagc
     3601 agggcaagtg gatgctgctg cgcgcgcagc tgcgcaacct ggaggatccg caggaccacg
     3661 ccgcgctgga agcgatcgcc aacgcgcgca cccaggaccc cgcggcgccc gtcgactgat
     3721 ggctcgcaca gctcactccg gacggtcgcg cggtcttcgc gctgatcgaa aataccgatc
     3781 cgcagcgggt cgacgcgctc gtcgccgcat tgccgcaacg cgtgcgcgag gaacttgcgg
     3841 cgctcgatct ggcgcgccac gacctcggag cgttgcgcgc gcaattgctg ctggtgcacg
     3901 gcgccgccga tccggtgatt ccactcagcc aaagcaagcg gttggccggg gcgctgccgg
     3961 aaggacaggt cgaattgttc atcccgccgg acttgagcca tgtggatctc gatcccggcc
     4021 tgcgcgatgc ctggcatctg tggcgcgcca cgacgcggct gctggcccat gcggaagcca
     4081 gtcggtgaac tgccttacgt gttcttgcgc gccagctgct tctcgaacgc ctgatccagc
     4141 ttgctcacct tcaccttctt ctgcgggccg gtgcgattgg ccagctcgat gaactcttcc
     4201 atcgtcagat cgtcgctgac gcgctcggcc ggctggcctt ccttcgcgcg cagcaggtag
     4261 aggaaaccgc tcgcagtcat gcccatcgag taacggtatt caggatcgcg cgcattcagg
     4321 cgcgtaacgg cggcgctggc tttggtggtt cgcatggcgg atgcggggag aaaatcgtca
     4381 gtttagatcg caacgccatc cggaagttgt ggcacgggct gcacgcttcg cttggccggc
     4441 ggataatgcg ctggtcgtag aacaac
//
LOCUS       DATLGF010000221        12589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_56225,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000221 DATLGF010000000
VERSION     DATLGF010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 12589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12589
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56225"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VM406_06670"
     CDS             <1..525
                     /locus_tag="VM406_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HVL75681.1"
                     /translation="TAFDEFALKAFEVHAVDYLLKPVRAARLATAIARAATARSAARE
                     RLQALTAELAAVRRHFSIQERGRVRLVPVDEVVYCKAELKYVTLRTRQQEYLVEESLT
                     SIEEEFGDRFVRIHRNALVARAAIAGVERGVQPVDGEDGEKTQEAWQVILRDIDERLP
                     ISRRQWGVVKALVR"
     gene            complement(578..3412)
                     /gene="ppc"
                     /locus_tag="VM406_06675"
     CDS             complement(578..3412)
                     /gene="ppc"
                     /locus_tag="VM406_06675"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HVL75682.1"
                     /translation="MPNKMQRAAQPPAAADKDAPLKEDIRLLGRLLGTVLREQEGEDV
                     FQVVETIRQTAVRFRRESDAGAGADLDRLLKKLTREQTISVVRAFSYFSHLANIAEDQ
                     HHNRRRRAHQLAGSEPQRGSIAFALTKLDQAGVSGQAVRHFFRDALIAPVLTAHPTEV
                     QRKSILDAERGIARLLAERDTPLTPKELAKNTQLLHARIATLWQTRMLRYSKLTVADE
                     IENALSYYRITFLRELPELYEDIRQDVAAHFPQRQGISDFPAFLQMGSWIGGDRDGNP
                     NVNADTMRHALMRQSTAIFDFYLDEVHALGAELSISTLLTGVTPQLQALADASPDQSD
                     HRQDEPYRRALIGLYARLAATARELGAAHVLRQEVGSAAPYADADAFVADLRTVAASL
                     EANHGSPLQKPRLLSLIRAAEVFGFHLATLDMRQSSDVHESVIAELFAGAGVEPSYAG
                     LDEERKVELLLAELDTPRLLYSPYLDYSDQTVSELAIVRTAREVRRRYGERAIRNYII
                     SHTETVSDLLEVLLLQKESGLLRFDADAGGAAGAELMVIPLFETIPDLRCAPDIMERF
                     LALPKVREIVRRQGDLQEVMLGYSDSNKDGGFLTSNWELYKAETRLVALFDRAGVKLR
                     LFHGRGGTVGRGGGPSYDAILAQPPGTVNGQLRLTEQGEIIASKFSDPEIGRRNLELM
                     VAATLEASLTPAASEGRRARTLAQFEGIMAELSELAYRAYRNLVYETPGFTDYFFAAT
                     PIAEIAELNIGSRPASRKSSRRIEDLRAIPWGFSWGQCRLLLPGWYGFGSAVQAWLHE
                     DEKLYKSRLSQLRAMYREWPFFATLLSNMDMVLAKTDLAVASRYAKLVADRKLRNAIF
                     RRIADEHERASHFFALVTGARERLAQNPLLARSIKNRFPYLDPLNHLQVELIKRHRGA
                     SAEGRTLDERVRRGIHLSINGIAAGLRNTG"
     gene            3581..4522
                     /gene="hemC"
                     /locus_tag="VM406_06680"
     CDS             3581..4522
                     /gene="hemC"
                     /locus_tag="VM406_06680"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HVL75683.1"
                     /translation="MKASPPSRLVIASRESRLAMWQAEHVRARLAQLYPQCSVEILGM
                     TTQGDRILDRALSKIGGKGLFVKELEVAMSERRADLAVHSLKDVPMELPEGFALAAVL
                     EREDPRDAFVSNDYASLDALPDGAVVGTSSLRRQALVAARYPNLVVKPLRGNLDTRLG
                     KLDRGEYAAIILAAAGLKRLGLQERIRALIEPEQSLPAAGQGAMAIEILAERADLLEA
                     LAPLNDVPTEQAVSAERTVSRIFGGSCQIPLAAFAQVNGASMTLRAMVATPDGKRIAG
                     AEASGSSDQPEELGQQVAHALRERGAEDILAACRSQV"
     gene            4548..5327
                     /locus_tag="VM406_06685"
     CDS             4548..5327
                     /locus_tag="VM406_06685"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HVL75684.1"
                     /translation="MSASALPVVVTRPAAMAQPLADGLRAAGRDAVVLPLLEIVPIED
                     ATTLRECLARLDTYALVVFVSPSAIDIVLPMIASWPASAAVAVVGEGSRRALARHGVC
                     EDNARIFSPCDPQRSDSETLLQVLDLEALRGRRVLILRGDGGRELLGDALRGAGAQVE
                     QVTAYRRRAPIFNEARRELLQRLLDGGAIWLITSSEALRVLTGWVRDAFGAAGLTKLQ
                     AQRLIVPHPRIAETAGALGFSDITQTASGDEHLLAALQSRP"
     gene            5324..6457
                     /locus_tag="VM406_06690"
     CDS             5324..6457
                     /locus_tag="VM406_06690"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HVL75685.1"
                     /translation="MNEQPSTSNVNDDKRADAGAAPESAPAPAPAPVTPSSARPVHQV
                     LFGAVILLAALLLAQWWVSQREISGLREDVAQRLRTGETAQTEARVVLKSLQDDIKGV
                     HAKVNVIESRQSEAQAQQLALEQLYQELARTRDDWTLSEIEQILTMASQQLQLAGNVQ
                     GAIIALQNADARLARADTQQLVRVRRAIAGDLERLQALPSVDLTGIALRLDSVIGQID
                     AMPLLADEKPALPATEPKIQRRSAPVEGEGNADWLARIENAWHSWTSEMWTEIKQVIR
                     VRNVETSDALLLSPTQSYYARENLKLRLLNARLALMTRNENVFRNDLIAAQDAIARYF
                     DTRARQTQNVQAMLRQVQASQLAIELPTLADSLNAVRSYKATN"
     gene            6467..7663
                     /locus_tag="VM406_06695"
     CDS             6467..7663
                     /locus_tag="VM406_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HVL75686.1"
                     /translation="MRIFLSLVILFALAIGLALGARFNPGNVVFFYPPYRVDMSLNFF
                     LLLAGLSFVTLMLVLAAIRTTRQLPGRVAAYRREKREREGNKALREALKALFEGRFGH
                     AEKAAERAAQNGENAPLASLVAARAAHRMGQPERRDAWLARIQAEPAMNTARLMTSME
                     LAIDGHDPDSALEAARELNASGTRHIHALRLALKANERAKNWPEVLRLVRLLDKRNAL
                     HPALSRRLRELAYDDLLKRQAVDAESLASFWATVPSADRTRPHVALRAAAAFHETGLA
                     DEARAILENALAAEWDGRLVRAYANCAGAEGSPALLAQIERCENWLQSHPGDAELELT
                     LGTLCLRQKLWGKAQRHLEDALSSAAEPQTVREAHLKLAQLHENLGQSEQAAAHYRQC
                     ALGILY"
     gene            7791..8330
                     /gene="ppa"
                     /locus_tag="VM406_06700"
     CDS             7791..8330
                     /gene="ppa"
                     /locus_tag="VM406_06700"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HVL75687.1"
                     /translation="MSLSNVPPGRDVPNDFNVIIEIPMNADPIKYEVDKETGAIFVDR
                     FMGTAMHYPCNYGYVPQTISDDGDPVDVLVVTPFPLIPGVVVRCRPLGVLKMTDEAGG
                     DAKLLAVPVDKVLPIYTHWQKPEDMNPLRLQQIQHFFEHYKDLEPGKWVKVEGWAGPD
                     AAREEILNGVAAYNRQHSK"
     gene            8477..10102
                     /locus_tag="VM406_06705"
     CDS             8477..10102
                     /locus_tag="VM406_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HVL75688.1"
                     /translation="MVTPHSSIAASGDDDDSDAASQARDRLLEKITAQSDLPALGASV
                     SKVVQMTSSDDEAVRNLAHFILADVALTQKILRIANSVTYRNASSTPVTTVSKAIFML
                     GFETVKTCALAMLLVDRMSTRQARNVRNELAHALCASIVGREMARRGPYKDAEEAAIA
                     SLFKNIGRVLVAAHEHECFVEISRLVEAGRHTPAQASHQILGCSFDTLGEAVLREWNI
                     PDPIVAAMIAPPAGVLKPARSRQEWVQQVAAFSAASARLIPGMSDPGQEAALKAVLTR
                     FGGALGLDQGRLLQLYAQVSQESRTLLSTTGLQGGRDNELAAADAEAGGTHQASSPRN
                     AGRDGFGDVLGDYVLEATGASAALAVTHRYPSGKPLNARDLLLAGVQDVTQMMSSGRW
                     RANDLILLVLETLYTSMGFRFATVCLKDLQSGQFRSRIALGESREAYQSGFAFPAAPT
                     RDLFHLAMENDADLMISDARVPKIRDLLPSWHRLLLPDARSFIVLPLVVQKRQLGLFY
                     ADRVEAAPEGVPPDETALIKTLKGQVLTALNAR"
     gene            complement(10108..11160)
                     /locus_tag="VM406_06710"
     CDS             complement(10108..11160)
                     /locus_tag="VM406_06710"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HVL75689.1"
                     /translation="MSTHAAQRASSQNRAISRVGRFHIIGELGRGSTGHVNLAHDPVM
                     GRNVALKVLNPGLGGAERQRHANCLINEARAAGRLSHPHIVTIYDASNEGGMPYIAME
                     HLQGRTLHQILAEGRRYCAVDTASIALRLAGALDHAHAQQVVHRDVKPANIFMVNDEH
                     PKLLDFGIARAPNRIPEDDDQTAHTLYRPNAALGTPNYMSPEQAQGKPVDERSDIYSL
                     GAVMYQMLAGRTPFEESDPDKLLQQIAHKAPRSLHEAVPGLHPELCSIVTRAMAKRPE
                     KRYQSAAELGVDLKRFLSRVRRAQTSGRAPAHPARAAVAQAAEAPGTMLKPQLRSWQV
                     LFGGVLAVTGLVYLFS"
     gene            complement(11245..12375)
                     /locus_tag="VM406_06715"
     CDS             complement(11245..12375)
                     /locus_tag="VM406_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL75690.1"
                     /translation="MNYRTRIISSLAEIGQTEWDNLAQSHAEANPFLSFAFLHALHES
                     GSASPATGWQPQYLTLWAGEALQAALPLYVKSHSYGEYVFDWAWADAYRRHGLAYYPK
                     LLSAIPFTPVAGPRLLARDARARSALVQALCALQDGNAFSSTHVLLPPEDEARELEAA
                     GFILRSSVQFHWLNAGYRDFQDFLDALERKKRKNIRAERRQVREAGVTFRHVGGEDAT
                     ERDWRFFKRCYDRTYATHYSTPYLNLDFFLRIGAAMPQHLLLVIAERGGDAIAASLLV
                     HNRRALYGRYWGALEYLPCLHFETAYYQPLEFCIARGIQRFEGGAQGEHKMARGLLPH
                     ALHSAHWLAHPGFAEAINEFTRREGRGIAAYLDELNEHNPFR"
     gene            12422..>12589
                     /locus_tag="VM406_06720"
     CDS             12422..>12589
                     /locus_tag="VM406_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HVL75691.1"
                     /translation="MTVKVAIAQINSTVGDFAGNHARIAEFARRAAAMGADIVVTPEL
                     SLAGYPPEDLLL"
BASE COUNT         2157 a         3996 c         4275 g         2161 t
ORIGIN      
        1 accgccttcg acgaattcgc gctcaaggcc ttcgaggtgc acgcggtgga ttacctgctc
       61 aagccggtac gggcggcgcg cctggcaacg gccatcgcgc gcgcagcgac cgcacgctcg
      121 gctgcgcgcg agcgcctgca ggcgctgacg gcggagctcg ccgcggtgcg ccggcatttc
      181 tccatacagg aacgcggccg cgtgcggctc gttccggtgg acgaggtcgt gtactgcaag
      241 gccgagctga agtacgtgac gctgcgcacg cgccaacagg aatacctggt ggaggaatcg
      301 cttacctcga tcgaggagga attcggcgac cgcttcgtgc gcatccaccg caacgcgctg
      361 gttgcgcgcg cggcgatcgc gggcgtggag cgcggcgtgc agccggtcga cggagaggac
      421 ggcgagaaga cgcaggaagc gtggcaggtg atcctgcgcg atatcgatga acgtctgccg
      481 atctcgcgca ggcagtgggg agtggtcaag gcgctggtgc gttgaacgaa cgcggcgtag
      541 cgcccgcgaa cgcatcgcac gatgcccggc ccggctttta accggtattg cgcagcccgg
      601 ccgctatccc gttgatcgac aagtggatgc cgcgtcgcac ccgctcgtcc agggtacgtc
      661 cctccgcgct cgcgccgcgg tggcgcttga tcagctccac ctgcaggtga ttcagcgggt
      721 cgaggtaggg gaagcggttc ttgatcgagc gcgccagcag cggattctgc gcgagacgct
      781 cgcgcgcacc ggtcaccagc gcgaagaaat ggctggcacg ctcatgttcg tcggcaatgc
      841 gcctgaagat ggcattgcgc agcttgcggt ccgcgaccag cttcgcgtag cgcgaggcga
      901 ccgcgaggtc ggtcttggcc agcaccatgt ccatgttgga gagcagagtg gcgaagaacg
      961 gccattcccg gtacatcgcg cgcagctggg aaaggcggct cttgtacagc ttctcgtctt
     1021 cgtgcagcca ggcttgcacc gcgctgccga agccatacca gccgggcagc agcaagcggc
     1081 actggcccca ggagaatccc caaggaatgg cgcgcaggtc ctcgatgcgg cggctggact
     1141 tgcgcgaggc cgggcgcgat ccgatattga gttcggcaat ctcggcgatc ggcgtggcgg
     1201 cgaagaagta gtcggtgaat cccggcgtct catagacaag attgcgatag gcccgatagg
     1261 cgagctcgga cagttcggcc atgatgcctt cgaactgcgc gagcgtgcgc gccctgcggc
     1321 cctcgctcgc ggccggcgtc aggctcgctt ccagggtggc ggcgaccatc agctcgaggt
     1381 tgcggcgccc gatctccggg tcggagaact tcgacgcgat gatttcgccc tgctcggtca
     1441 ggcgcagctg accgttcacg gtgccgggcg gctgggcgag gatcgcgtcg tagctggggc
     1501 cgccgccgcg cccgacggtt ccgccgcgtc catggaacag ccgcagcttg acgccggccc
     1561 gatcgaacag cgcaaccagc cgcgtctcgg ccttgtacaa ctcccagttg gaagtcagga
     1621 agcccccgtc cttgttcgaa tccgagtagc cgagcatcac ttcctgcagg tcgccctggc
     1681 ggcgcacgat ctcgcgcacc ttgggcagcg cgaggaagcg ctccatgatg tcgggcgcgc
     1741 agcgcaggtc gggaatcgtc tcgaacagcg gaatcaccat cagttcggcg ccggccgcgc
     1801 cgcctgcatc ggcgtcgaag cgcagcagtc cgctttcctt ctgaagcagc agcacctcga
     1861 gcaggtccga caccgtctcc gtatgcgaga tgatgtagtt gcggatggcc cgctcgccgt
     1921 agcggcgccg cacttcacgc gcggtgcgca cgatggccag ttcggatacc gtctggtccg
     1981 agtagtcgag gtagggggaa tacagcaggc gtggcgtatc cagttcagcc agcagcagct
     2041 cgaccttgcg ctcctcgtcc aggcccgcat agctcggttc cacgccggct ccggcaaaca
     2101 gttcggcaat cacgctctcg tgcacatccg agctttggcg catgtcgagc gtggccaggt
     2161 ggaagccgaa cacttccgcg gcgcggatca gcgagagcag gcgcggcttc tgcagggggc
     2221 ttccgtggtt cgcttcgagc gaggcggcga ccgtgcgcag atcggcgacg aaggcgtcgg
     2281 catccgcata cggcgcagcg ctgccgactt cctggcgcag cacatgcgcg gcgccgagct
     2341 cgcgcgccgt ggcggccagg cgggcataga ggccgatcag cgcacgccgg tacggttcat
     2401 cctggcgatg atcggactgg tcgggagagg cgtcggccaa cgcctgcagc tggggcgtga
     2461 cgccggtcaa cagcgtggag atcgatagtt ccgcgccgag ggcatggacc tcgtccaggt
     2521 agaagtcgaa gatggcggtc gattgccgca tcagcgcatg gcgcatcgtg tccgcgttga
     2581 cgttcggatt gccgtcgcgg tcgccgccaa tccagctgcc catctgaagg aaggcaggga
     2641 agtcacttat gccctgtcgc tgcgggaagt gggccgcgac atcctggcga atgtcttcat
     2701 acagctcagg cagttcgcgc aggaaggtga tccggtaata agacagcgca ttctcgatct
     2761 cgtcggccac cgtcagcttc gagtagcgca gcatgcgggt ctgccagagg gtcgcgatgc
     2821 gggcgtgcag cagctgggtg ttcttcgcca gctctttcgg cgtgagcggc gtgtcgcgtt
     2881 ccgccagcag gcgggcaata ccgcgctcgg cgtccaggat gctcttgcgc tgcacctcgg
     2941 tcgggtgcgc ggtcagcacc ggcgcgatca gcgcgtcgcg gaagaagtgg cgcaccgcct
     3001 ggccggacac cccggcctga tccagcttag tcagcgcgaa cgcgatgctg ccgcgctgcg
     3061 gctcggagcc cgccagctgg tgggcgcggc ggcgacggtt gtgatgctgg tcctcggcaa
     3121 tattggccag atgggagaaa tacgagaacg cgcggaccac ggaaatggtc tgctcgcgcg
     3181 tgagcttctt cagcagacga tccaggtccg ccccggcgcc ggcatcggac tcgcgtcgaa
     3241 agcgcacggc cgtctggcgt atggtttcca cgacctggaa cacgtcttcg ccttcctgtt
     3301 cgcgcagcac ggtgccgagc aggcggccga gcaggcggat gtcttccttc aacggggcgt
     3361 ctttgtctgc cgcggccggc ggctgcgctg cgcgctgcat cttgttgggc atgtgaaatc
     3421 ctgcgtcgct ggggtggcac tcgtgaggct ggacaagcgt ggaatcccgg aacgggcccg
     3481 gcgaggatcg tgaacgcatc ccctgccggc tgggtaggag gctcaagggt gcatgataag
     3541 atgcgggtcc gcgcagtacc agacatcgaa agaacccgca gtgaaagctt ctcccccttc
     3601 cagattggtc atcgcctcgc gcgagagtcg tctcgcgatg tggcaggccg agcatgtgcg
     3661 tgcacggctg gcgcaattat atccgcagtg cagtgtggaa attctcggaa tgacaacaca
     3721 aggggatcgc atcctcgacc gcgcgttgtc gaaaatcggc ggcaagggcc tgttcgtcaa
     3781 ggagctcgag gtggccatgt ccgaacgccg tgcggacctg gcggtgcatt cgctcaagga
     3841 cgtgccgatg gagctgccgg aaggttttgc gctggccgcg gtactggagc gggaagatcc
     3901 gcgcgatgct ttcgtatcga acgactatgc gagtctcgat gcgctgccgg acggcgcggt
     3961 ggtcggcacc agcagcctgc gtcgccaggc gctggtggca gcgcgctatc cgaatctcgt
     4021 ggtcaaaccg ctgcgcggca atctggacac gcggctgggc aagctcgacc gcggcgagta
     4081 tgccgccatc attctcgctg cagccggctt gaagcgtctt ggccttcaag aacgcatccg
     4141 cgcgctgatc gaacccgaac agagcttgcc ggcggccggg cagggtgcga tggcaatcga
     4201 gatcctcgcc gagcgcgccg atctgctcga ggcgctggcg ccgctcaacg atgtgccgac
     4261 cgagcaggcc gtgagcgccg aacgcaccgt ttcgcgcatt ttcggcggca gctgccagat
     4321 tccgctggct gcgttcgcgc aggtcaatgg cgcgtccatg acgctgcggg caatggtcgc
     4381 cacgccggac ggcaagcgca ttgccggcgc cgaggcgtcc ggctcaagcg accagcccga
     4441 agagctcggc cagcaggtcg cgcacgcgct gcgcgaacgc ggcgccgagg acatcctggc
     4501 cgcctgccgg tcccaggttt gaaccgattc tcctggcagg cacacagatg agcgccagcg
     4561 cattgcccgt cgtcgtgacg cgtcccgcgg caatggccca gccgctggca gacggcctgc
     4621 gtgctgccgg ccgcgacgcc gtcgtgctgc ccttgctcga aattgttcca attgaagatg
     4681 cgaccacgct gcgcgaatgc cttgcgcgcc tggatacata cgcgctggtt gttttcgtga
     4741 gcccgagcgc aatcgacatc gtgctgccga tgatagcttc ctggccggcc tcggctgccg
     4801 tggcagtggt gggcgaggga agccgccgag cgcttgcccg gcatggcgtg tgcgaggaca
     4861 acgcgcgcat tttcagtccc tgcgacccac agcgcagcga ctccgagacc cttctgcagg
     4921 tgctcgacct tgaggcgttg cgcgggcggc gggtattgat cctgcgtggc gacggcggcc
     4981 gcgagctgct tggcgacgcg ctgcgcgggg ccggtgccca ggtcgagcag gtgacggcct
     5041 atcgacgcag ggcccccatc ttcaacgagg cgcggcgcga actgctgcag cgcctgcttg
     5101 acggcggcgc aatctggctg atcacgagtt ccgaagcgct gcgcgtgctg accggatggg
     5161 tacgagacgc gttcggcgcg gcgggcctga caaaactgca agcgcagcgc ctgatcgtgc
     5221 cgcatccgcg catcgccgag accgccggcg cactcggatt ttccgatatc actcagaccg
     5281 cttcagggga cgaacacctg ttggccgcgc tacaatcccg cccatgaacg aacagccttc
     5341 cacttcgaac gtcaacgatg acaagcgggc cgacgccggc gccgctccgg aatcggcacc
     5401 ggctcccgcg cctgccccgg tcactccatc cagcgccagg ccggtacatc aggtcctgtt
     5461 cggcgcggtc atcctgctcg cggcgctgct gctggcgcag tggtgggttt cgcagcgcga
     5521 gatcagcggc ctgcgcgaag acgtcgccca gcggctgcgc accggggaaa ccgcccagac
     5581 cgaagcgcgc gtggtgctca agtcgctgca ggacgatatc aagggcgtgc atgccaaggt
     5641 caatgtgatc gaaagccggc agagcgaggc gcaggcccag cagctcgcgc tcgaacagtt
     5701 gtaccaggaa ctggcgcgca cccgcgacga ctggacgcta tccgagatcg agcagattct
     5761 gacgatggcg tcccagcagc tgcaattggc aggcaacgtg cagggcgcaa tcatcgccct
     5821 ccagaatgcc gacgcacgcc tggcccgcgc cgacacccag cagctggtgc gcgtgcgccg
     5881 tgcgatcgcc ggcgatctgg agcgtctgca ggcgcttcca tcggtcgacc ttaccggcat
     5941 tgcgctgcgc ctggacagcg tgatcggcca gatcgacgcg atgcctctgc tggcggacga
     6001 gaagccggca ctgcctgcaa ccgagccgaa aatccagcgt cgctccgccc cggtcgaggg
     6061 cgaggggaac gcagactggc ttgcccggat cgagaacgcc tggcacagct ggaccagcga
     6121 gatgtggacc gagatcaagc aggtgatccg ggtgcgcaac gtcgaaacct ccgacgcgct
     6181 gctgctgtcg ccgacgcagt cctattacgc ccgcgagaac ctgaaactgc gtctgctcaa
     6241 cgcgcggctg gcgctgatga ctcgcaacga gaatgtgttc cgcaatgacc tgatcgccgc
     6301 gcaagacgcg atcgcccgct atttcgacac gcgcgcccgc cagacccaga acgtgcaggc
     6361 gatgctgcgg caggtgcagg ccagccagct cgcgatcgaa ctgccgacgc tggccgatag
     6421 cctgaacgcc gtgcgcagtt acaaggccac caactagccg accgcaatgc gcatcttcct
     6481 ctcgctcgtc atcctgttcg cgctcgccat cggcctggcc ctcggcgcgc gcttcaaccc
     6541 gggcaatgtg gtgttcttct acccgccgta tcgggtggac atgtcgctca atttcttcct
     6601 ccttctcgcg gggttgtctt tcgtgacgct gatgctggtg ctggcagcga tccgcacgac
     6661 tcggcagctg ccggggcggg tcgccgcgta tcggcgggaa aagcgtgagc gggaaggcaa
     6721 caaggcactg cgtgaagcat tgaaggcatt gttcgagggg cgcttcgggc atgccgagaa
     6781 ggcggcggaa cgcgcggcgc agaatggcga aaacgcgccg ctggcgtcgc tggtcgcggc
     6841 gcgtgccgcg cacaggatgg ggcagccgga gcggcgcgac gcctggctgg cgcgcatcca
     6901 ggccgagccg gccatgaaca ctgcgcggct gatgacctcg atggagcttg ccatcgacgg
     6961 ccacgatccg gattccgcgc tggaggcggc gcgcgagctc aatgcaagcg gcacgcgcca
     7021 catccacgcg ctgcggctcg cgctcaaggc caatgaacgc gcgaagaact ggcctgaagt
     7081 gctgcgcctg gtgcgcctgc tcgacaagcg caacgcgctg catccggcgt tgtcgcgccg
     7141 cctgcgcgaa ctggcctacg acgacctcct gaaaaggcag gctgtcgatg cggaatctct
     7201 ggccagtttc tgggcgacgg ttccgagcgc ggaccgcaca cggccgcatg ttgcgctgcg
     7261 tgcggccgcc gcattccacg aaaccggtct ggccgacgaa gcgcgcgcaa ttctcgagaa
     7321 cgcgctggcc gccgagtggg acggccgcct cgtgcgtgcc tatgcgaact gcgcgggagc
     7381 ggaaggctct ccggccctgc tggcgcagat cgagcgctgc gagaactggc tgcagtcgca
     7441 cccgggcgat gccgaacttg agctgaccct cgggacgttg tgtctgcgcc agaagctgtg
     7501 gggcaaggcg cagcggcatc tcgaggatgc cctctcgtcg gctgcagagc cgcaaacggt
     7561 gcgcgaggcg catctgaagc tggcccagtt gcacgaaaac ctcgggcagt ccgaacaggc
     7621 cgctgcgcat taccgacagt gcgcgctcgg catcctctat tgatccgctt ccggcctcgt
     7681 ccggtggccg gttgatcctg ctcgaagccc ctgttgcaat gcaaaagatg caggggaaac
     7741 tcgctataat cgcgggttct gaaccttcca tcacttccag agagagcaat atgagcttga
     7801 gcaacgtgcc gcctggccgc gacgttccga acgacttcaa cgtcatcatc gagatcccga
     7861 tgaacgcgga tccgatcaag tacgaagtgg acaaggaaac cggcgcgatc ttcgtcgatc
     7921 gtttcatggg cactgccatg cactatccgt gcaattacgg ctacgttccc cagacgattt
     7981 ccgacgacgg cgatccggtt gacgtgctgg tcgtgacccc gttcccgctg attccgggtg
     8041 tcgtggtgcg ctgccgtccg cttggtgtac tgaagatgac ggatgaagcc ggcggcgacg
     8101 ccaagctgct cgcggtgccg gtcgacaagg tactgccgat ctacacgcac tggcagaagc
     8161 cggaagacat gaatccgctg cgcctgcagc agatccagca tttcttcgag cactacaagg
     8221 acctcgagcc gggcaagtgg gtcaaagtcg aaggctgggc agggccggac gctgcccgcg
     8281 aggaaatcct gaatggcgtg gccgcctata atcgccagca cagcaagtaa tcgcagcatc
     8341 tgcgacccga ctgcaagatg ggcgcgccag ccggtgcgcc cttcgttttt tccgcgcgcg
     8401 actgcctgcc tcgtcgacaa gaccgggcga atgcgtcatc attgccccca cgcaatctat
     8461 cgggaacctt gcttcagtgg tcactccaca ctccagcatc gccgcttcgg gggatgacga
     8521 cgacagcgat gccgccagcc aggcacgtga tcgtcttcta gaaaaaatca ccgcccagag
     8581 cgacctgccc gccctcgggg cctctgtttc caaggtcgtg cagatgacct cgtcggacga
     8641 cgaggcggta cgcaaccttg cacatttcat cctggccgat gtcgcgctga cccagaagat
     8701 tctccggatc gcgaattcgg tcacctaccg caacgcttcg agcacgcccg tcaccaccgt
     8761 ttccaaagcc attttcatgc tcggctttga aaccgtgaag acctgtgcgc tggccatgct
     8821 gctcgtcgat cgcatgtcca cccgccaggc gcgcaacgtg cgcaacgaac tggcgcatgc
     8881 gctgtgcgcg agcatcgtcg ggcgcgaaat ggccaggcgc ggtccgtaca aggacgcgga
     8941 ggaagcggcg atcgcctcgc tgttcaagaa catcgggcgt gtgctggtgg ccgcgcacga
     9001 gcatgaatgt ttcgtcgaga tttcccgcct ggtcgaggcg gggcgccaca cgcccgcgca
     9061 ggcgtcgcac cagatcctcg gctgcagctt cgataccttg ggcgaggctg tgctgcgtga
     9121 gtggaacatt cccgacccca tcgtggcggc gatgatcgcg ccgccggccg gcgtcctgaa
     9181 acccgcgcgt tcgcgccagg agtgggtgca gcaggtggcg gctttcagcg cggcttccgc
     9241 gcgcctgatt ccaggcatga gtgatcccgg gcaggaggcg gcgctcaagg cggtgctcac
     9301 gcgattcggc ggcgcgctcg gattggatca ggggcggcta ttgcagctgt acgcccaggt
     9361 gagccaggag tcccgcacgc tgctgagcac caccggcctg caagggggac gggacaacga
     9421 gctcgcggcg gctgacgcgg aggcgggcgg cacccaccag gcgtcttcgc cgaggaatgc
     9481 cggccgtgac ggcttcggcg atgtcctcgg cgattacgtg ctcgaggcta ccggcgcatc
     9541 ggcagcgctc gccgtcacgc accggtatcc gagcggaaag ccgctcaatg cgcgcgactt
     9601 gctgcttgcc ggggtacagg atgtcacgca gatgatgtcg tccgggcgtt ggcgcgccaa
     9661 cgacttgatc ctgcttgtgc tggaaacgct ctacacgagc atgggctttc gctttgcgac
     9721 ggtctgcctc aaggatctgc agtccgggca gttccgttcg cgcattgcgc tcggcgagag
     9781 tcgcgaggcg tatcagtccg gctttgcatt tccggctgcc ccgacccgcg acctgttcca
     9841 ccttgcgatg gaaaacgacg cggacctgat gatctccgat gcccgggtgc cgaagatccg
     9901 cgacctgctt ccgtcatggc atcgcctgct gctgcctgac gcgcgcagtt tcatcgtcct
     9961 gccgctggtc gtgcagaaga ggcagctggg cttgttctac gcagatcgcg tggaagcggc
    10021 gcccgaaggg gtgccgcccg atgaaaccgc actgatcaag accttgaaag ggcaggtgct
    10081 gaccgcgctg aacgcgcgct gaggccctca gctgaacagg tagacgagtc cggtgaccgc
    10141 caggacgccg ccgaacagca cctgccagga acgcagctgc ggcttgagca tggtgccggg
    10201 agcctcggcc gcctgcgcga ccgccgctcg tgcgggatgg gcgggagcgc gccccgatgt
    10261 ctgagcgcgg cgcacacggg aaagaaagcg cttcagatcg acgcccagct cggcggcact
    10321 ctgataccgc ttctccggcc gcttggccat cgccctcgtc acgatactgc acagttccgg
    10381 atgcagcccg ggcacggctt cgtgcagact gcggggcgcc ttgtgcgcga tttgctgcag
    10441 gagcttgtcc gggtcgcttt cctcgaacgg tgtgcggccg gcaagcatct ggtacatcac
    10501 cgcgccgagg gaatagatgt ccgaacgttc gtcgaccggc ttgccctgcg cctgttcggg
    10561 cgacatgtag ttcggtgtgc ccagcgcagc gttcggccgg tacagcgtat gtgcagtctg
    10621 gtcatcgtcc tcggggatcc ggttcggcgc gcgggcgatt ccgaaatcca gaagtttcgg
    10681 atgctcgtcg ttcaccatga agatgttggc cggtttcaca tcgcgatgca cgacctgctg
    10741 ggcatgggcg tgatcgagcg cgccggcgag cctcagggcg atggacgccg tgtccacagc
    10801 gcagtaccgt cgcccttccg cgaggatctg gtgcagggtg cgtccctgca gatgttccat
    10861 cgcgatatac ggcatgccgc cctcgttcga ggcgtcgtag atggtgacga tgtgcggatg
    10921 ggacaagcgg ccggcagcgc gcgcttcgtt gatcaggcag tttgcatgac gctggcgttc
    10981 ggcgccgcca aggccgggat tcaggacctt gagcgcgaca ttgcgcccca tgaccgggtc
    11041 atgtgcgagg ttgacatggc ctgtggagcc gcgtccgagt tcgccgatga tatggaaacg
    11101 acccacacgc gagatcgccc ggttttggga ggaggcgcgt tgcgcggcat gcgtgctcat
    11161 ggtgcgagag catgccgtga agtgagtgtt ttggcaacgc gatttctggt agaagagtgc
    11221 aggaaaaatc ttgacttgtg gttgtcagcg gaacggattg tgttcgttca gttcgtcgag
    11281 ataggcggca atgccgcgtc cttcgcggcg ggtgaattcg ttgattgcct cggcaaagcc
    11341 gggatgggcc agccaatgcg cggaatggag cgcgtgcggc aggaggccgc gtgccatctt
    11401 gtgctcgccc tgcgcgccgc cttcaaagcg ctggatgcct cgcgcgatgc agaattccag
    11461 cggctggtag taggcggtct cgaagtgcag gcaggggagg tattcgagcg cgccccaata
    11521 gcggccatac agcgcgcggc ggttgtggac gagcagggag gcggcgatcg cgtcgccgcc
    11581 gcgttcggcg atgaccagca acaggtgctg cggcatcgcg gctccaatgc gcaggaagaa
    11641 gtcgaggttg aggtagggtg tcgaatagtg ggtcgcgtag gtgcggtcat agcagcgctt
    11701 gaagaagcgc cagtcgcgct cggtcgcgtc ctcgccgccg acgtggcgga aggtgacgcc
    11761 ggcctcgcgg acctggcggc gctcggcgcg gatgttcttg cgctttttac gctcgagcgc
    11821 gtcgaggaag tcctggaagt cgcgatagcc ggcattgagc caatggaatt gcacgctgct
    11881 gcgcaggatg aagccggccg cctccagttc gcgggcctcg tcctcgggcg gcaacagcac
    11941 atgggtggac gagaatgcgt tgccatcctg gagcgcgcaa agcgcctgca ccagcgcgct
    12001 gcgggcgcgg gcatctcgcg cgagcaggcg cgggccggca accggcgtaa acgggatcgc
    12061 ggacaggagt ttcgggtaat acgcgagacc atggcgccga tacgcgtcgg cccaggccca
    12121 gtcgaacaca tactcgccgt aggaatgcga cttcacatac aggggcaggg cggcttgcag
    12181 cgcttccccc gcccacagcg tcaggtactg cggctgccat ccggtcgccg gactggcgct
    12241 gccgctttca tgcaaggcat gcaggaaggc aaaggacagg aaaggattcg cctccgcgtg
    12301 gctctgggcc agattgtccc attcggtttg cccgatttcg gccagagacg aaatgattcg
    12361 cgtgcgataa ttcactcttg ctaaatcgta tgtgcggcgt gatcctgccg caatttcaga
    12421 gatgacagtc aaggtcgcca tcgcccagat caacagcact gtcggtgact ttgccggcaa
    12481 tcatgcgcgc attgcggagt ttgcccggcg cgccgccgcc atgggggccg acatcgtcgt
    12541 cacgccggaa ctttcgttgg ccggctatcc gcccgaggac ctgttgttg
//
LOCUS       DATLGF010000222         3405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_194334, whole genome shotgun sequence.
ACCESSION   DATLGF010000222 DATLGF010000000
VERSION     DATLGF010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194334"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VM406_06725"
     CDS             complement(<1..369)
                     /locus_tag="VM406_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75692.1"
                     /translation="MRIDVQFADRVGIAHEILAVLARRSMNVVAVEVDSSDVFIDIPE
                     LTEDLLPSLREDFLRIPGVRGISVVDVLPGARQRLHLDTLLAVMADPVMAVDAGGRVV
                     AVNAAAAALTATTRPPASTAI"
     gene            complement(451..987)
                     /locus_tag="VM406_06730"
     CDS             complement(451..987)
                     /locus_tag="VM406_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75693.1"
                     /translation="MNAAAAERAMIPPFIFGDPQLHARFSTAITLMTQIMRAAPAGVP
                     TAQLAATLGQSARALAPVLRALCAASLIERADDVRASGEAWLSRRNPRGITLADLFCS
                     VANMPGNTNRSPAGVDDDGHCAARQAVELLLMQATMSINQVVLQRLQAFDLERLTAIG
                     RKGALDSVYARTSLRTLA"
     gene            complement(984..2279)
                     /locus_tag="VM406_06735"
     CDS             complement(984..2279)
                     /locus_tag="VM406_06735"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HVL75694.1"
                     /translation="MKVIVLGSGVIGTTAAYYLAQAGHEVSVIDRQPGPGLETSYANA
                     GEVSPGYSAPWAAPGIPLKAIKWLLMRHSPLVIRPSLDPAMWRWVLMMLRNCTSARYE
                     LNKSRMVRLAEYSRDCLIALRRHTGIHYDERMQGTLQLFRTQKQLDNAASDIAVLERY
                     GVPYELLDRAGCARAEPALAHVRDKYVGALRLPNDETGDCFKFTQRLAELAAQDGVKF
                     SYGAGIHGLNASGDRLVSVRTSEGEQKADAYVLALGSYSPLLLREVGIRIPVYPVKGY
                     SITVPIHEAAHAPESTVMDETYKVAITRLGDRIRVGGTAEIAGYDLSLRNARRATLEH
                     SVCDLFPRGGDVARAEFWAGLRPMTPDGTPVMGPTPLRNLFLNTGHGTLGWTMACGAG
                     RVLADLVSGRRPEIDTEGLFLDRYGRINRPIIIPREVRA"
     gene            complement(2298..3404)
                     /gene="alr"
                     /locus_tag="VM406_06740"
     CDS             complement(2298..3404)
                     /gene="alr"
                     /locus_tag="VM406_06740"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HVL75695.1"
                     /translation="MGAVCSNYRLLRAQAPLAECAAVLKADAYGLGAEAVGEALAAQG
                     CRLFFVAHLEEGIVLRAALPAAAQVFVLHGPVPGSEREFLRHNLVPVLNTPGQIDAWR
                     ALARSVGRALPAILQVDSGMARLGLTPVEVDALLERGDGLRGIALRYLMSHLACAEEQ
                     AHPMNAAQLARFTQLRSRFPAVPASLANSSGIFLGPRFHFDLLRPGAALYGIAPVAGA
                     VNPMRPVVRLQAGVIQVREIEGGASVGYGARYRAPGRRRIATIAAGYADGWLRSLSGR
                     GSAVIDGKKLPMVGTVSMDTCALDVTDLPAARVQPGTLVDLICADQPVDAVAAMAGTI
                     GYEILTSLGSRYRRRYIGGAQNDETSAHEAATSV"
BASE COUNT          549 a         1163 c         1181 g          512 t
ORIGIN      
        1 gatggcggtc gacgcggggg ggcgggtggt agcggtcaac gccgccgcgg cggcgttgac
       61 cgctaccacc cgcccccccg cgtcgaccgc catcaccgga tcggccatca cggccagcag
      121 cgtgtcgagg tgcagccgct ggcgggcccc cggcagcacg tcgacaacgc tgatgccgcg
      181 cacgcccgga atgcgcagga agtcctcgcg cagcgatggc agcaggtctt cggtgagctc
      241 agggatgtcg atgaacacgt cggaggagtc gacctcgacg gcgacgacgt tcatgctgcg
      301 ccgcgcaagg acggcgagga tctcgtgggc aatgccgaca cggtcggcga actggacgtc
      361 tatacgcatc tggaagcatg aggccggcag ccaacgaagg gcactttaac cgccggtcgc
      421 ggttcggaca agagcgcgcc cgcaaaaggc ctaggcgagc gtgcgcaggc tggtgcgcgc
      481 gtacacgctg tccagcgcgc ctttgcggcc gatggcggtc aggcgttcga gatcaaacgc
      541 ttgcaggcgc tgcagcacga cctggttgat cgacatcgtc gcctgcatca gcagcagttc
      601 gaccgcttgc cgcgcagcgc aatgcccatc gtcgtcgacg ccggccggcg accggttcgt
      661 gttgcccggc atattcgcga cgctgcagaa caggtcggcc agggtgatgc ctcgtggatt
      721 gcgccgcgac agccaggcct cacccgacgc gcgcacatca tcggcccgtt cgatcaggct
      781 ggccgcgcac agcgcgcgca ggacaggcgc cagcgcccgc gcggactgcc ccagcgttgc
      841 ggccagctgt gcggtcggca cgccggcggg cgcggcgcgc atgatctggg tcatcagggt
      901 gatggcggtg gagaagcgcg catgcagctg cgggtcgccg aagatgaacg gcgggatcat
      961 cgcgcgctcc gctgccgccg cgttcatgcg cgcacctcgc gcgggatgat gatcggacgg
     1021 ttgatgcgcc cgtagcggtc gaggaaaagt ccttcggtat cgatctccgg ccgccgcccg
     1081 gagacgagat ctgccagcac gcgccccgcg ccgcaggcca tggtccatcc cagcgtgccg
     1141 tggccggtgt tgaggaacag gttgcgcagt ggtgtcggcc ccatcaccgg cgtgccgtcc
     1201 ggcgtcatcg gacgcaggcc tgcccagaac tcggcgcgcg cgacatcgcc gccgcgcggg
     1261 aacagatcgc agacggaatg ctcgagcgtc gcgcggcgcg cgttgcgcag gctcaggtcg
     1321 tagcccgcta tctccgcggt cccgccgact cggatgcgat cgcccaggcg ggtgatcgcc
     1381 accttgtagg tctcgtccat cacggtcgac tcgggcgcat gggccgcctc gtggatcggc
     1441 accgtgatcg aatagccctt gactggatag accggaatgc ggatccctac ctctcgcagc
     1501 agcagcggcg aatagcttcc cagcgccagc acataggcat ccgccttctg ctcgccctcg
     1561 gacgtgcgca ccgacaccag cctgtcgccg ctggcgttga ggccgtgaat gcctgcgccg
     1621 tagctgaact tgaccccgtc ctgcgcggcc agctcggcca gccgctgtgt gaacttgaag
     1681 cagtcgccgg tctcgtcatt gggcaggcgc agtgcgccga cgtacttgtc gcgcacatgg
     1741 gcgagcgcgg gctccgcgcg tgcgcatccg gcgcgatcga gcagctcgta gggtacgccg
     1801 tagcgctcga gcaccgcgat gtcgctggcg gcgttgtcga gctgcttctg ggtgcggaac
     1861 agctgcagcg tgccctgcat ccgctcgtcg tagtggatgc cggtgtgccg acgcaacgcg
     1921 atcaggcagt cgcggctgta ttcggccagg cgcaccatgc ggctcttgtt gagttcgtac
     1981 cgggccgacg tgcagttgcg cagcatcatc agcacccagc gccacatcgc cggatcaagg
     2041 ctgggccgga tcaccagcgg actgtgccgc atcagcagcc acttgatcgc cttgagcggg
     2101 ataccgggtg cagcccaggg ggcggaataa cccggcgaca cctcgccggc gttcgcgtag
     2161 ctggtctcca gcccgggacc gggttgccgg tcgatcacgc tgacctcatg gccggcctgc
     2221 gcaagatagt aggcggccgt ggtgccgatc acgccgctgc cgagtacgat gactttcacg
     2281 ggtttcctct cgggtggtca aacgctggtt gcggcctcgt gggccgaagt ctcgtcgttc
     2341 tgcgcgccgc cgatgtaccg gcggcggtaa cgcgaaccga ggctggtaag gatctcgtag
     2401 ccgatcgttc ccgccatcgc cgcgaccgcg tcgaccggct ggtccgcgca gatcagatcg
     2461 accagcgttc cgggttgcac gcgcgccgca ggcagatcgg tgacgtcgag cgcacaggta
     2521 tccatcgaca ccgtgccgac cataggaagc ttcttgccgt caatgaccgc gctgccgcgc
     2581 ccgctcaggc tgcgcagcca gccgtctgcg tagccggcgg cgatcgtcgc gatgcggcgc
     2641 cgccccggcg cccgatagcg cgcgccgtag cctacgctgg cgccgccttc gatttcgcgg
     2701 acctggatca cgcctgcctg caggcggacg accggtcgca tcggattgac cgccccggct
     2761 acaggtgcga tcccatacag cgccgcgccc gggcgcagca gatcgaaatg aaagcgcggc
     2821 ccgaggaaga tgccggagga attggcaagg ctggcgggaa ctgccggaaa gcgggaccgc
     2881 agttgcgtaa aacgtgcaag ctgggctgcg ttcatcgggt gcgcctgctc ctcggcgcag
     2941 gcgaggtgac tcatcaggta gcgcagcgca atgccgcgca ggccgtcgcc gcgctccagc
     3001 agcgcatcga cctcgaccgg cgtaaggccc agccgcgcca tcccgctatc gacctgcagg
     3061 atggcgggca atgcgcgccc gacgctacgt gcaagcgcgc gccacgcgtc gatctggccc
     3121 ggggtgttga gcaccggaac gaggttgtga cgaaggaatt cgcgctcgga acccggtacc
     3181 ggtccatgca gcacgaagac ctgggctgcg gcaggcaatg ccgcccgcag cacgatgccc
     3241 tcttcgaggt gggcgacgaa gaacaggcgg cagccctgcg cggcgagcgc ctcgccgacc
     3301 gcctcggcac ccaggccata ggcatcggcc ttcagcaccg ccgcgcattc ggccagcggc
     3361 gcctgcgcac gcagcaggcg gtagttcgaa cagacggcac ccagg
//
LOCUS       DATLGF010000223         2138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_100696, whole genome shotgun sequence.
ACCESSION   DATLGF010000223 DATLGF010000000
VERSION     DATLGF010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100696"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1294)
                     /locus_tag="VM406_06745"
     CDS             complement(<1..1294)
                     /locus_tag="VM406_06745"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HVL75696.1"
                     /translation="MPPTLLIRNAHILVTMDAARREIPDGAVFIRGNVIEQVGTSAEL
                     PQTADEVIDAAGHVVLPGLVNTHHHMFQSLTRAIPAAQDAELFGWLQALYPIWAGLTP
                     EMVQVSTLTAMTELLLSGCTTTSDHLYLYPNGCTLEDSIEAAEQIGMRFHAARGAMSV
                     GESQGGLPPDALVEDEAAILADMQRLIERWHDPSRHAMRRIVLAPCSPFSVSRDLMRE
                     SARLARAYGVSLHTHLAENISDVAYSREQFNMTPAQYAEDCEWLGHDVWHAHCVQLDA
                     HGIDLFARSRTGVAHCPCSNMRLASGIAPVRRMLDAGVPVGLGVDGSASNDGAHMLGE
                     TRQAMLLQRVGFGPDALGARQALELATLGGARVLGRDDIGALAPGMAADIVMFGLDSI
                     GFAGALHDPVAALVFCAPAQVACSVINGRVVVRDGRCTT"
     gene            complement(1515..>2138)
                     /locus_tag="VM406_06750"
     CDS             complement(1515..>2138)
                     /locus_tag="VM406_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HVL75697.1"
                     /translation="IVTSIAGSVRYLVELTGVASHAGTTPMSMRKDAAAAAAEIILYV
                     EKRCTQAPSLVGTVGQLQVPNGSVNVIPGACRLSLDVRAADDATRDAALADILREIEA
                     ICARRRIEYKLDLLMKAPAVPCAPWLMRQLAAATESTGLAPFELTSGAGHDAMAIAAI
                     TDVAMLFTRCGNGGISHNPLETMTADDAEISAQVFLRFLKDFQAQGL"
BASE COUNT          337 a          732 c          732 g          337 t
ORIGIN      
        1 cggtcgtgca gcgcccgtcg cgtaccacga cccggccatt gatcacgctg cacgccacct
       61 gtgccggcgc gcagaacacc agcgccgcga ccgggtcgtg caaggccccg gcaaagccga
      121 tcgaatccag gccgaacatc acgatatcgg ccgccatgcc gggagccagc gcgccgatgt
      181 cgtcacggcc gagcacgcgc gcgccgccca gggtcgcgag ttcgagcgcc tggcgcgcgc
      241 ccagcgcatc cgggccgaag ccgacccgct gcagcagcat ggcctggcgc gtctcgccca
      301 gcatgtgggc accatcgttg gaggcggaac cgtccacacc gaggccgacc ggcacgccgg
      361 catccagcat ccgccgcacc ggtgcgatgc ccgaagccag ccgcatattg gagcaggggc
      421 agtgggcgac gccggtgcgg ctgcgcgcga acagatcgat gccgtgcgcg tcgagctgca
      481 cgcagtgcgc atgccagacg tcgtggccga gccactcgca gtcctcggcg tactgtgccg
      541 gcgtcatgtt gaactgctcg cggctgtagg cgacgtcgga gatgttctcg gcgaggtggg
      601 tgtgcaggct gacgccgtag gcacgcgcca gccgggccga ttcacgcatc aggtcgcgcg
      661 agaccgaaaa cggcgagcag ggcgcgagca cgatgcgccg catcgcgtgc cgcgacggat
      721 cgtgccagcg ctcgatcagg cgctgcatgt ccgcgaggat cgcagcttcg tcctccacca
      781 gcgcatccgg cggcagcccg ccctgcgact cgcccacgct catcgcgccg cgcgccgcat
      841 gaaagcgcat gccgatctgc tcggccgcct cgatgctgtc ttccagcgtg cagccgttcg
      901 gatacaggta gaggtgatcg ctggtggtcg tgcagccaga cagcagcagt tcggtcattg
      961 ccgtcagcgt cgacacctgc accatctccg gcgtgagtcc tgcccagatc gggtaaagcg
     1021 cctgcagcca gccgaacagc tccgcatcct gcgcggcggg tatcgcacgc gtgaggctct
     1081 ggaacatgtg gtggtgcgtg ttgacgagac cgggcagcac gacgtggccg gccgcatcga
     1141 tcacttcgtc cgccgtctgc ggcagttcag cgctggtgcc gacctgctcg attacgttgc
     1201 cgcggatgaa gacggcgcca tccggaatct cgcgtcgggc cgcgtccatc gtgacgagga
     1261 tgtgggcgtt gcggatgagg agggtggggg gcatgggggg cgatttagag tcgggttgtt
     1321 tcagtcgaac ggctgctctt gtgctgacgt tctgcggcgc tcaagcgcgt caggaccgat
     1381 gtgccttgca tgtcgctcta ctccccgcgg cggcccgcgc aggggaacga cctagcgcgg
     1441 agtgggcgcg aaacgaacct cgtcggtgcc atgtcgatct ctccttccac aagaagatcg
     1501 acccactccc atctctacaa cccctgcgcc tggaaatcct tcaggaaccg caggaacacc
     1561 tgcgccgaaa tctcggcatc gtccgcggtc atcgtctcca gcgggttgtg gctgatcccg
     1621 ccgttgccgc agcgggtaaa cagcatcgcc acgtcggtga tggcggcaat cgccatcgca
     1681 tcgtggccgg cgccggaggt cagctcgaac ggggcgaggc cggtgctttc cgttgccgcc
     1741 gccagctggc gcatcagcca cggcgcgcag ggcacggccg gcgctttcat cagcaggtcc
     1801 agcttgtact cgatccggcg acgtgcgcag atcgcctcga tctcgcgcag gatgtcggcc
     1861 agtgccgcgt cgcgcgtcgc gtcgtcggcc gcgcgcacgt cgagcgagag ccggcaggcg
     1921 ccggggatga cgttgaccga gccgttgggc acctggagct ggccgacggt tccgaccagc
     1981 gacggcgcct gcgtgcagcg tttctcgaca tacaggatga tctcggccgc cgccgccgcc
     2041 gcgtccttgc gcatgctcat cggcgtggta ccggcgtggc tggccacacc ggtcagttcg
     2101 accaggtagc ggacgctgcc cgcaatcgag gtcacgat
//
LOCUS       DATLGF010000224         8776 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_116626, whole genome shotgun sequence.
ACCESSION   DATLGF010000224 DATLGF010000000
VERSION     DATLGF010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 8776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8776
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116626"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..598)
                     /gene="recX"
                     /locus_tag="VM406_06755"
     CDS             complement(146..598)
                     /gene="recX"
                     /locus_tag="VM406_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HVL75698.1"
                     /translation="MAKVQMSLKARALKYLSAREHSRMELARKLARYAGEGDDIEALL
                     DSLAAANLLSEERFSESLVRRRAARFGTSRIVSELQSHGIDAEAVESMRQSLSEDECM
                     RARAVWQRKFGTPPADAAERARQMRFLQQRGFSSAAIRQALRPSDPDD"
     gene            complement(827..1870)
                     /gene="recA"
                     /locus_tag="VM406_06760"
     CDS             complement(827..1870)
                     /gene="recA"
                     /locus_tag="VM406_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HVL75699.1"
                     /translation="MDDKKSLSNPDKSKALAAALAQIEKQFGKGSVMRMVDGAVAEEV
                     QVVSTGSLGLDIALGVGGLPRGRVVEIYGPESSGKTTLTLQAIAEMQKLGGTCAFIDA
                     EHALDVVYAQKLGVNLSDLLISQPDTGEQALEITDALVRSGSVDLVVVDSVAALTPRA
                     EIEGDMGDSLPGLQARLMSQALRKLTGSINRTNTLVIFINQIRMKIGVMFGNPETTTG
                     GNALKFYASVRLDIRRTGSIKSGDEVIGNETKVKVVKNKVAPPFREAHFDILYGEGTS
                     REGEILDLGVEAKIVEKSGAWYSYNGERVGQGKDNARNYLKDNPLLAREIENKVRAAL
                     GVRELPPVAAEAG"
     gene            2091..2792
                     /locus_tag="VM406_06765"
     CDS             2091..2792
                     /locus_tag="VM406_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL75700.1"
                     /translation="MRILLAEDDSVLADGLTRSLRQSGYATDCVKTGLEADSALSTQD
                     FDLLILDLGLPRMPGLEVLRRLRARNSRLPVLILTAGDSVEQRVKGLDLGADDFMAKP
                     FALSELEARVRALTRRGMGGGPTVIRHGPLSYDQVGRIAYINDQMLDLSARELGLLEV
                     LLQRSGRLVSKEQLVDHLCEWGEEVSNNAIEVYVHRLRKKIEVGGVRIATVRGLGYCL
                     EKHVEPAASGASTDA"
     gene            2792..4357
                     /locus_tag="VM406_06770"
     CDS             2792..4357
                     /locus_tag="VM406_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HVL75701.1"
                     /translation="MPSVSDAAPQAAGNAHAPLPDASPALPAPLPTKPQERVQRSLFG
                     EILDWMLVPLLLLWPMSIAITYLVAKSIANQPFDRALEDRTMVLAQQVKEVDGRIATR
                     LSASAREILRADEVDQVHYQILGSRGELADGDRDLPLPPQQARVSTTQVQFRNDVVRG
                     NEVRVAWMHVDLRHDRSGRAVLRDEYLALVQVAETLEKRAHLANEVIKGVILPQFVIL
                     PIALALVWLALARGLTPLAEIQRRIRERRPDDLSPIETGQVPEEIAPLVHSFNDMLGR
                     LSQSIEMQKRFIADAAHQMKTPLAGMRTQAELALRESDPVEVRRSLVQLAKSSETAAR
                     MVNQLLALARAENASPATKPFHPLELRELARAVVQDWVQTSFAQRIDLGYEQPDHPVT
                     IVGNPVMLREMLSNLIDNALHYTPSGGSVTVRIGIDALERKVILEVEDTGPGIPAAER
                     SRVFERFYRILGSNVDGSGLGLAIVREIVQQHGATVQIHDNPHSQDPRFPGTLIIVLL
                     PLFQRPALTEDIG"
     gene            4357..4638
                     /locus_tag="VM406_06775"
     CDS             4357..4638
                     /locus_tag="VM406_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/substrate symporter small subunit"
                     /protein_id="HVL75702.1"
                     /translation="METMRESAKGNLPGTRYWRAARRATAALLLAWFGVTFVTLFFAR
                     ELADLRIFGWPFSFYMAAQGLILFYLLLVALYVGLMRRLDRMLKDDHAA"
     gene            4628..6664
                     /locus_tag="VM406_06780"
     CDS             4628..6664
                     /locus_tag="VM406_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HVL75703.1"
                     /translation="MLRDPFFWRLTRYYGLYTAAFVGFLVLVALLERRGFPRSLIGYL
                     LIFVTILLYASIGVLSRTADVAEYYVAGRRVPALFNGMATAADWMSAATFLSLAGGLY
                     VMGFDGLAYIVGWTGGYCLVAFLIGPYLNKFGQYTIPDFLAARYSGDDGGRTVRMLAV
                     FATVLVSFTYVVAQIYGVGLIASRFTGVDFTVGIFLGLASILVCSFLGGMRAITWTQV
                     AQYIVIIIAYMTPVVWLSIKHTGAAVPQVAYGSVLDRLSEREAELNSDPGEQQVREVF
                     RARAAEYDARLAALPLSWEQGLREAKGRVDELKRSGASLAEVKAATRAVAAYPRSPDD
                     ARRVWSEARNANLTRAAPVAPHATPFPGETKEASDIRRNNFLALVLCLMMGTAALPHI
                     LVRFYTAPNVQQARSSVLWTLFFIMLLYITVPALAVLVKIDIYTSLVGSDFAQLPAWV
                     SYWANVDKAQPLVAIADLNGDGIVQLAEIVMDGDIVVLATPEIAGLPYVVSGLIAAGG
                     LAAALSTADGLLLTISNALSHDVYYRSVRPEASTQRRVTVSKLLLLAVALVAAYVASL
                     KPGDILSMVGAAFSLASSTLFPALALGVFWSRANRAGAIAGMVTGFCVCVYYMVTTYP
                     ALGGSSANQWFHIAPISAGIFGVPAGFGAMLVVSLLTRAPDQSNSVLLQHLRSP"
     gene            complement(6745..7788)
                     /gene="purM"
                     /locus_tag="VM406_06785"
     CDS             complement(6745..7788)
                     /gene="purM"
                     /locus_tag="VM406_06785"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HVL75704.1"
                     /translation="MSNTSKVSLSYRDAGVDIDAGDALVEAIKPFAKRTMREGVLGGI
                     GGFGALFEIGRKFKEPVLVSGTDGVGTKLKLAFHLNRHDTVGIDLVAMSVNDILVQGA
                     EPLFFLDYFACGKLDVAIATDVIKGIAQGCEQAGCALIGGETAEMPSMYPDGEYDLAG
                     FAVGAVEKSKIIDGSRIAPGDVVLGLASSGAHSNGYSLVRKIISVAQPDLNADFHGRP
                     LADVLLAPTRIYVKPLLALMQSIEVKGMAHITGGGLVENVPRVLGDRLTAVLQRDAWT
                     MPPLFSWLQQHGGVADAEMHRVFNCGIGMVVIVGKDQADAAIAQLQAAGETVSKIGEI
                     RERQGDEAQTIVI"
     gene            7950..8633
                     /gene="hda"
                     /locus_tag="VM406_06790"
     CDS             7950..8633
                     /gene="hda"
                     /locus_tag="VM406_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HVL75705.1"
                     /translation="MRQLPLDLGAAPPQTLANFAVGQNAELLQLLHACEARAQAMPLE
                     RFIYLWGESGAGKTHLLHALAGADDARYFHGDVPATALEYEPGVALYLLDDCHRLSPD
                     AQIAAFALFNQVRDNGGLMVTAGDLPPASLTLREDLRTRLGWGLIYQVHSLTDEDKVE
                     ALQQIAQARGLNVSAGVLPYLITHFRRDMGSLAAMLDALDRYSLETRRPVTLPLLRDL
                     LQQEAQAQE"
     gene            8633..>8776
                     /locus_tag="VM406_06795"
     CDS             8633..>8776
                     /locus_tag="VM406_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HVL75706.1"
                     /translation="MNLALFDLDHTILPLDSDYEWGQFLVRIGAVDADEFARRNAEFY
                     AQYE"
BASE COUNT         1470 a         2959 c         2738 g         1609 t
ORIGIN      
        1 gcaaagtgta gcacagcttg caaacggcaa gccaagggga aaatgtccgc gaagcaacgg
       61 actcgctcgc tctccactgc cggcacggaa gagaaccggc ggccatggtt gaaccgcctt
      121 ggcgcggcgg gactcgcatg taccgtcagt cgtcggggtc agaggggcgc agcgcctggc
      181 ggatcgccgc ggaagaaaag ccgcgctgct ggaggaagcg catctgccgg gctcgctccg
      241 cggcatcggc gggcggagtt ccgaacttgc gctgccagac ggcccgcgcg cgcatgcact
      301 catcttcgga aagcgactgg cgcatcgatt cgacggcttc cgcgtcgatg ccatgggact
      361 ggagttcgga gacgatgcgg ctggtgccaa aacgggccgc gcggcggcgc accaaagatt
      421 cggagaaccg ctcctcggac agaagattgg ccgcggcaag cgagtccagc agcgcctcga
      481 tatcatcgcc ctcgccagcg tagcgcgcca gcttgcgcgc caattccatg cggctgtgct
      541 cacgcgccga caaatacttc agggcccgcg ccttcagact catctgcacc tttgccatgg
      601 tgatctgccg acacgggccc tttcacgtga agtaagcggg cttgttgctc aatgcttgcg
      661 aaccacgcag tcatgaagat cgcgctgcac taatcgttca agcgcgccgc ctcggcttac
      721 agtcgaacat gcagtgcatc gctgcatgag ctgttcgccg tactcggcgc gcccgccgtg
      781 gttcaagtta tccagtcatt cttgcgacac aactactcaa agccgctcag cctgcctccg
      841 cagccaccgg cggcaattcg cgcacgccaa gcgccgcgcg gaccttgttt tcgatctcgc
      901 gcgccagcag cgggttgtcc ttcaggtaat tgcgcgcatt gtccttgccc tggccgacac
      961 gctctccgtt gtagctgtac caggcgcccg atttctcgac aatcttcgct tcaacgccca
     1021 gatccaggat ctcgccttcg cgtgaggtac cttccccgta caggatgtcg aaatgcgctt
     1081 ccctgaacgg gggcgcgacc ttgttcttca cgaccttgac cttggtctcg ttgccgatga
     1141 cctcatcgcc ggacttgatc gagccggtgc ggcggatatc caggcgcacg gaggcataga
     1201 acttcagcgc gttgccgccg gtcgtggttt ccgggttacc gaacatgacg ccaatcttca
     1261 tccggatctg gttgatgaag atgaccagcg tgttcgtgcg gttgatgctg ccggtgagct
     1321 tgcgcagggc ctgggacatc aggcgggcct gcaggccggg cagggaatcg cccatgtcgc
     1381 cttcgatttc ggcacgcggg gtcagggcgg cgaccgagtc gaccacgacc agatccacgc
     1441 tgcccgagcg caccagcgca tcggtgatct ccagcgcctg ttcgccggta tccggctgcg
     1501 agatcagcag gtcggacaag ttgacgccca gcttctgcgc gtagacgacg tcaagcgcgt
     1561 gctccgcgtc gatgaatgcg caggtgccgc cgagcttctg catttccgcg atggcctgca
     1621 gggtgagcgt ggtcttgcct gaggactccg ggccgtagat ttcgaccacc cggccgcgcg
     1681 gcaggccgcc gacgcccagt gcgatatcca atccgagcga tccggtcgag acgacctgaa
     1741 cctcctcggc caccgctccg tcgaccatgc gcatgacgga gcccttgccg aactgcttct
     1801 caatctgggc gagcgcggcg gccagcgcct tgctcttgtc cgggttggac agggatttct
     1861 tgtcgtccat gagaattctt tccgagaatg aatgtgtagc cggatcgcct tgtgcaatgc
     1921 cgggaaagtg ggtgcatgcg cagcactgta caaataaaca gtgaaggatg caagtgccgc
     1981 gtcgagttgc acgaaattta acacagcgca atgtcggcgc aaaagccgcg cgccttgccg
     2041 ggcattctga aacgcaatag acttgttcga cttacacgag ccagtcgccc atgcgtatcc
     2101 tgcttgccga agacgatagt gtactcgccg acggactgac ccgctccctg cgccagtccg
     2161 gctatgcgac ggactgcgtc aagaccgggt tggaggcgga ttcggcgctg tccacccagg
     2221 atttcgactt gctgatcctc gatctcggcc tgcccaggat gccaggtctc gaggtgctgc
     2281 gccgcctgcg cgcacgcaat tcccggctgc cggtgctgat cctgaccgcc ggcgattcgg
     2341 tcgaacaacg ggtcaagggg ctcgatcttg gcgccgacga tttcatggcc aagccgtttg
     2401 ccctctccga actcgaagcg cgcgtgcgcg cgctcacgcg gcgcggcatg ggcggcggcc
     2461 cgaccgtcat ccgccatgga ccgctgtcgt acgaccaggt cgggcgcatc gcctacatca
     2521 acgaccagat gctcgacctt tccgcgcgcg aactgggtct gctcgaagtg ctgctgcagc
     2581 gcagcggccg gctggtatcg aaggagcagc tggtcgatca cctgtgcgaa tggggcgagg
     2641 aagtctcgaa caacgcgatc gaggtgtatg tacaccggct gcgcaagaag atcgaggtcg
     2701 gcggcgtacg gatcgccacg gtgcgcggcc tcggttactg cctcgagaag cacgttgaac
     2761 cggcggcttc cggcgcgagc acggacgcct gatgccgagc gtgagcgacg ctgcgccaca
     2821 ggccgccggc aacgcccacg ctcccctccc cgacgcctcg cccgcgcttc ccgcgccgct
     2881 tcccaccaaa ccgcaggagc gcgtccagcg ctcgctgttc ggcgaaatcc tcgactggat
     2941 gctggtgccg ctgctgctgc tgtggccgat gagcattgcc attacctacc tggtcgccaa
     3001 gtcgatcgcg aaccagccct tcgaccgcgc gctggaagac cggaccatgg tcctggccca
     3061 gcaggtcaag gaggtcgacg gccgcatcgc cacacggctg tcggcttccg cgcgcgagat
     3121 cctgcgcgcc gatgaggtgg accaggtcca ttaccagatc ctcggctcgc gcggcgagtt
     3181 ggccgacggc gaccgcgatc tgccgctgcc gccgcagcag gcgcgcgtct cgaccaccca
     3241 ggtccagttc cgcaacgacg tggtgcgagg caacgaggta cgcgtggcgt ggatgcatgt
     3301 cgacctgcgc catgaccgca gcggacgagc cgtgctgcgc gacgaatatc tggcgctggt
     3361 ccaggtggcg gagacgctgg agaagcgggc tcatctcgcg aacgaggtga tcaagggcgt
     3421 gatcctgccg cagttcgtga tcctgccgat cgccctcgcg ctggtctggc ttgcgctggc
     3481 gcgcgggctc acgccgctcg ccgagatcca gcgacgcata cgcgagcgcc gtccggacga
     3541 tctctcaccg atcgagaccg gacaggtacc ggaggagatt gcgccgctgg tgcactcatt
     3601 caacgacatg ctgggacgcc tgtcgcagag catcgagatg caaaagcgct tcatcgccga
     3661 cgccgcccac cagatgaaaa cgccgctggc cggcatgcgt acgcaggccg agcttgcctt
     3721 gcgcgaatcc gatccggtcg aggtgcgccg ctcgctcgtg cagctcgcga agagctcgga
     3781 aaccgccgcc cgcatggtga accagttgct tgcgctcgca cgggccgaga atgcctcccc
     3841 cgccaccaag cccttccatc cgctcgagct gcgcgaactg gcgcgcgcgg tggtgcagga
     3901 ttgggtgcag acgtccttcg cccagcgcat cgacctgggc tacgaacagc cggaccatcc
     3961 ggtcacgatc gtcggcaatc cggtcatgct gcgcgaaatg ctgagcaatc tgatcgacaa
     4021 tgcgctgcac tacacgccgt ccggcgggtc ggtgactgtc agaatcggca tcgatgcact
     4081 ggagcgcaag gtcatcctcg aggtcgagga taccggcccg ggcattccgg ccgccgaacg
     4141 cagcagagtg ttcgagcgct tctatcgcat cctcggcagc aatgtcgacg gcagcggcct
     4201 gggccttgcg atcgtgcgcg agatcgttca gcagcacggt gcaaccgtgc agatccacga
     4261 caatccgcac agtcaggacc cgcgtttccc gggcacgctg atcatcgtcc tgctgccgct
     4321 gttccagcgc cccgccttga ccgaggacat cggctgatgg agaccatgcg cgaatccgcc
     4381 aagggcaatc tgccgggcac ccggtactgg cgcgcggccc gccgcgcgac cgccgcattg
     4441 ctgctggcct ggttcggcgt gaccttcgtc acgctcttct tcgcgcgcga actggccgac
     4501 ctgcgcatct tcgggtggcc gttctctttc tacatggcgg cgcaagggct gatcctgttc
     4561 tacctgctcc tggtcgctct gtacgtaggc ttgatgcgcc gcctggaccg catgctgaag
     4621 gacgaccatg ctgcgtgacc cgttcttctg gcgcctgacg cgctactacg gcctgtacac
     4681 cgccgcgttc gtcggcttcc tggtactggt tgcgctgctc gagcgccgcg gctttccgcg
     4741 ctcgctgatc ggatatctgc tgatcttcgt gaccatcctg ctctacgcca gcatcggcgt
     4801 gctcagccgt accgccgacg tggccgaata ctacgtcgcc ggccggcgcg tacctgcgct
     4861 cttcaacggc atggccaccg cggccgactg gatgtcggcc gcgaccttcc tcagtctggc
     4921 cggcgggcta tacgtgatgg gattcgacgg gctcgcctac atcgtcggct ggaccggcgg
     4981 ctactgcctg gtcgccttcc tgatcggccc ctacctgaac aagttcggcc agtacacgat
     5041 cccggatttt ctcgccgcgc gctacagcgg cgatgacggc ggccgcaccg tgcgcatgct
     5101 cgcggtgttc gccacggtgc tggtgtcgtt cacctatgtg gtcgcgcaga tctacggcgt
     5161 cggcctgatc gcctcgcgct tcaccggcgt cgatttcaca gtcgggatct tcctcggact
     5221 ggcaagcatc ctggtctgct catttcttgg cggcatgcgc gcaatcacct ggactcaggt
     5281 ggcccagtac atcgtcatca tcatcgccta catgacgccg gtggtctggc tgtcgatcaa
     5341 gcacaccggc gcggcggtgc cgcaggtggc ctacgggagc gtactcgacc ggctaagcga
     5401 acgcgaagcc gaactgaaca gtgatcccgg cgagcagcag gtacgcgaag tgtttcgcgc
     5461 cagagccgcc gagtacgacg cgcggctggc cgccctgccg ctctcctggg agcagggact
     5521 gcgtgaagcc aagggccggg tcgacgaact gaaacgatcg ggcgcctcgc tggcggaagt
     5581 gaaggcggcc acccgcgctg tggccgccta tcccagatcg ccggacgacg cccggcgggt
     5641 gtggagcgag gcgcgcaacg cgaacctcac ccgtgcggcg ccggtggcgc cacacgccac
     5701 gccgttcccg ggagaaacca aggaagcatc cgacattcgc cgcaacaatt tcctcgcgct
     5761 ggtgctgtgc ttgatgatgg gcacggcggc gcttccgcac atcctggtgc gcttctacac
     5821 tgcgcccaat gtgcagcagg cacgctcctc cgtgctgtgg acgctgttct tcatcatgct
     5881 gctgtacatc acggtcccgg cgcttgcggt gctggtgaag atcgacatct acacttcgct
     5941 ggtcgggtca gatttcgccc aactgccggc ctgggtctcg tactgggcaa acgtcgacaa
     6001 ggcccagccg ctcgtcgcga tcgcggattt gaacggggac ggaatcgtgc agctggcgga
     6061 aatcgtgatg gacggcgaca ttgtcgtgct cgccacaccc gagatcgccg gcctgcccta
     6121 tgtggtttcc gggctcatcg ccgccggcgg cctggcagcg gcgctgtcga ccgccgacgg
     6181 attgctgctg acgatctcca acgccctctc ccatgacgtc tattaccgca gcgtgcgccc
     6241 ggaagcgtcg acccagcgcc gggtcacggt ttccaagctt ctgcttcttg cggtggcgct
     6301 ggtggcggcc tatgtcgcct cgctcaagcc cggcgatatc ctgtcgatgg ttggcgcagc
     6361 attctcgctc gcgtcgtcca cgctgttccc ggcccttgcg ctcggcgtat tctggtcgcg
     6421 cgccaaccgc gcaggagcga ttgccggcat ggtcaccggc ttctgcgtct gcgtgtacta
     6481 catggtgacg acctaccccg ctctcggcgg ctcgagtgcg aaccagtggt tccacatcgc
     6541 cccgatttca gccgggatct tcggcgtccc cgcgggcttt ggcgcgatgc tcgtggtcag
     6601 cctgcttacg cgtgcgccgg accagagcaa ttccgtactg ctccagcatc tgcgctcgcc
     6661 ctgaaccgcc ttgcctgccg cgcccggcca caaaaaaagg cgaacgcaca gcagcgttcg
     6721 ccttgttcct gcccgagctg tcgctcagat cacgattgtc tgcgcctcgt cgccctggcg
     6781 ctcgcggatc tcgccgatct tcgataccgt ctcaccggcc gcctgcaact gcgcgattgc
     6841 cgcgtcggcc tggtccttgc cgacgatcac gaccatgccg atgccgcagt tgaatacacg
     6901 atgcatttcg gcatcggcca cgccgccgtg ctgctgcagc caggaaaaca gcggtggcat
     6961 cgtccaggcg tcgcgttgca ggacggcggt cagcctgtcg ccgagcacgc gcggcacgtt
     7021 ttcgaccagt ccgccgccgg tgatgtgggc catgcccttg acctcgatac tctgcatcag
     7081 cgccagcagc ggcttgacat agatgcgggt gggcgccagc agcacgtcgg ccagcgggcg
     7141 gccatggaag tcggcattca ggtccggctg cgcgaccgag atgatcttgc gcaccagaga
     7201 gtagccgttc gagtgcgcgc cggaagaggc cagacccagc accacgtcgc ccggtgcgat
     7261 cctgctgccg tcgatgatct tggatttctc caccgcgccg accgcaaagc cggccaagtc
     7321 atattcgccg tccggataca tgctcggcat ctcggcagtc tcgccgccga tcagcgcgca
     7381 gccggcctgc tcgcatcctt gggcgatccc cttgatcaca tcggtggcaa tggccacgtc
     7441 cagcttcccg caggcgaaat agtcgaggaa gaacagcggc tccgcgccct gcacgaggat
     7501 gtcgttgacg ctcatcgcaa ccagatcgat gccgacggtg tcatgccggt tcaggtgaaa
     7561 agccagcttc agcttggtgc cgacgccgtc ggtaccggag accagcaccg gctccttgaa
     7621 tttcctgcca atctcgaaca aggcaccgaa gccgccgatc ccgcccagca cgccctcgcg
     7681 catcgtgcgt ttggcgaagg gcttgatcgc ttcgaccagg gcatctccgg cgtcgatgtc
     7741 gaccccggcg tcgcggtagg aaagggaaac cttggaagtg ttgctcatga tatggagttc
     7801 gaggcggtag aataagcggt tttagccggt tgctttcggc aagtccgcta ttctaacaaa
     7861 ccgatccaac ccctccgatc ctccatgtca gtcaatcacg tgccgggacc gcgtatcgct
     7921 tccgtcccgc gtgccagcgt cgtaaagtca tgaggcagct cccgctcgac ctgggcgccg
     7981 cgccaccgca gacgctcgcg aatttcgccg tgggccagaa cgcggaactg ttgcagttgt
     8041 tgcatgcctg tgaggcccgc gcgcaggcga tgccgctcga acgtttcatt tacctgtggg
     8101 gtgaaagcgg cgcgggcaag acccacctgc tgcacgcgct ggccggcgcc gacgatgccc
     8161 gctacttcca cggagacgtg ccggcgacgg cgctcgaata cgaacccggc gtcgcgctct
     8221 atctgctgga cgactgtcat cgcctgtccc cggatgccca gatcgccgca ttcgccctgt
     8281 tcaaccaggt gcgcgacaac ggcggcctga tggtgaccgc cggcgacctt ccgcccgcct
     8341 cgctcacgct gcgcgaagat ttgcgcacgc gcctgggctg gggcctgatc taccaggtgc
     8401 acagcctgac cgacgaggac aaggtcgaag cgctgcagca gatcgcgcag gcgcgtggac
     8461 tgaacgtctc ggccggcgtg ttaccctatc tcatcaccca cttccggcgc gacatgggtt
     8521 cgctcgcggc catgctcgac gcgctcgacc gctactcgct cgaaacccgg cgccccgtca
     8581 ccctgccgct gctgcgcgac ctgctgcagc aggaagctca agcccaggaa taatgaatct
     8641 cgcactgttc gatctagacc acacgatcct gccgctcgac tcggattacg aatggggcca
     8701 gttcctggtg cgcatcggtg cggtcgacgc ggacgaattc gcgcgccgca acgccgagtt
     8761 ctatgcacag tacgag
//
LOCUS       DATLGF010000225         4200 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_4919,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000225 DATLGF010000000
VERSION     DATLGF010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4919"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VM406_06800"
     CDS             <1..524
                     /locus_tag="VM406_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HVL75707.1"
                     /translation="DGQREDLIKTADALIVADNRRTLPEFVIETVLARRLHPQAGRAV
                     PVRYHDLREIRADCALLLSLVAHVGAANVAAEPDDLFLEGAAQCPQLQLRREDLLAAS
                     AIDFIAARDALERAHQLAPLAKPVLIKALLAAGGRRDPMPVGLADLLRAICAAIEAPV
                     PPAVADSYTAHHW"
     gene            587..1111
                     /locus_tag="VM406_06805"
     CDS             587..1111
                     /locus_tag="VM406_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HVL75708.1"
                     /translation="MQASAIPSKKPLWIKLVATVLALAIALAAYFSLFARQTAPDVTF
                     SSLTGEQITMQSLRGKVVVVNFWATSCVTCVKEMPALVQTYEKYKGQGLDLIAVAMNY
                     DPPNYVVNFAQTRQLPFTVALDHTGQVAHSFNDVKLTPTTYVIGKDGQIIKRYVGEPD
                     FAAFHQLIEKALAA"
     gene            complement(1152..2126)
                     /gene="lipA"
                     /locus_tag="VM406_06810"
     CDS             complement(1152..2126)
                     /gene="lipA"
                     /locus_tag="VM406_06810"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HVL75709.1"
                     /translation="MSTDKQPLASTAYNPSEKQKGAGKTARIPIKIVPSEKLAKPDWI
                     RVKAASPSTRFYEIKDILRANKLVTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCP
                     FCDVGHGRPDPLDAEEPENLARTIAALKLSYVVITSVDRDDLRDGGAAHFAECIRRVR
                     EQSPHTRIEILTPDFRGRMDRALEILKAAPPDVMNHNLETVPRLYKEARPGSDYAYSL
                     SLLKRFKELHPDTPTKSGLMVGLGETDEEILQVMRDLRAHDVDMLTIGQYLMPSGDHL
                     PVRRYVHPDTFKMFEEEAYKMGFAHAAVGAMVRSSYHADLQAHQAGVA"
     gene            complement(2161..2796)
                     /gene="lipB"
                     /locus_tag="VM406_06815"
     CDS             complement(2161..2796)
                     /gene="lipB"
                     /locus_tag="VM406_06815"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HVL75710.1"
                     /translation="MLPTLIDRGHEPYETTYAAMRAFTDARSADTRDELWIVEHPPVF
                     TLGLGADPSHVLDANGIPVVQTDRGGEVTYHGPGQVVIYLLMDLRRRPGKRLFAREFV
                     HKIEQAVIDTLATYNLDGERKAGAPGIYVSDGRWKGAKIAALGLKVRGNGCTYHGVSL
                     NVGMDLRPFSGINPCGYAGMATVDMRTLGVERPLHEVQQALARTLIEYLKS"
     gene            complement(2800..3642)
                     /locus_tag="VM406_06820"
     CDS             complement(2800..3642)
                     /locus_tag="VM406_06820"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVL75711.1"
                     /translation="MPGSLPARTLTAQREFGENLRMLCSYYRSVADVCRKLNINRAQF
                     NRYLNGTSRPSNHTLERICEFFGVEPSEIHLPHQQFRQVVQVHPRQRSEHLAYVDHIA
                     RLQQQPSARNDKYLGYYYEYYHSMSAPGKVLRGLVRIFLHEGAVYHERLERFPQHANL
                     DQTYRCKYLGATFFLNDRIFLTDYESLSGNEIAHTVLYPTYKNRVARLSGLMLGVSAS
                     NQRAIACTRVVFEFLGKSIDVRAALRRCGLFDPAEGAVDRTILKVIDNSAHKDRHHFY
                     TIPL"
     gene            3893..>4200
                     /locus_tag="VM406_06825"
     CDS             3893..>4200
                     /locus_tag="VM406_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75712.1"
                     /translation="MLPFDDCILGNSALHARFLATTEILARLVSSAPRKVHLTQLEDD
                     TGRCAAEITACCAALGRAGLLTVHDGHEPAWSLAGTPALVTLEDVFCCVMNEQMPRAR
                     "
BASE COUNT          713 a         1313 c         1387 g          787 t
ORIGIN      
        1 ccgatggcca gcgcgaagac ctgatcaaga cggccgacgc gctgatcgtc gccgacaacc
       61 ggcgcacctt gccggagttc gtgatcgaaa ccgtgctggc ccggcgcctg cacccgcagg
      121 ccgggcgcgc ggtgccggtg cgctatcacg atttgcgcga gatccgcgca gactgcgcgc
      181 tgctgctgtc gctggtggcc catgtcggcg cggccaacgt cgccgccgag ccggacgacc
      241 tgttcctcga aggtgccgcc cagtgcccgc aactgcaact gcggcgggaa gacctgctcg
      301 ccgcctcggc gatcgatttc atcgccgccc gcgacgcgct cgagcgcgcg caccagctcg
      361 ccccgctggc caagccggtg ctgatcaagg cgctactggc cgcgggcggg cggcgcgatc
      421 cgatgccggt cgggttggcc gatctgctgc gggcgatctg tgcggccatc gaagcgccgg
      481 ttccgcccgc agtggccgac agctataccg cccatcactg gtagccggca tgcgggcggt
      541 tcgaagtcta taattgggcc gtcccctcct cccgcatatc cacgccatgc aagcctccgc
      601 cattccgtcc aagaagccgc tctggatcaa acttgtcgca accgtgctcg cactggcgat
      661 cgccctggct gcctatttct cgctgtttgc gcgccagacc gcgccggacg tcactttttc
      721 cagcctgacc ggcgagcaga tcacgatgca gagcctgcgc ggcaaggtgg tggtcgtgaa
      781 cttctgggca accagttgcg tgacctgcgt gaaggaaatg ccggccctgg tgcagaccta
      841 tgagaagtac aaggggcagg ggctggactt gatcgcagtt gcgatgaact acgacccgcc
      901 gaactacgtg gtgaatttcg cacagacgcg ccagctgccg tttaccgttg cgcttgacca
      961 taccgggcag gtcgcgcatt cgttcaatga cgtcaagctc acgcccacca cctacgtgat
     1021 cggcaaggat ggccagatca tcaagcgcta cgtcggcgag ccggatttcg cggccttcca
     1081 ccagttgatc gagaaggcgc tggcggcctg aggccgcggc cgggcgctgg ttcgccgagg
     1141 ggcaaccgac ctcaggccac cccggcctga tgcgcctgca ggtccgcgtg gtacgagctg
     1201 cgcaccatcg cgccgacggc cgcgtgggca aaccccatct tgtaggcttc ttcctcgaac
     1261 atcttgaacg tgtccggatg cacgtagcgg cgcaccggca ggtggtcgcc ggagggcatc
     1321 aggtactggc cgatggtcag catgtcgaca tcgtgtgcgc gcaggtcgcg catcacctgc
     1381 aggatttcct catcggtctc gcccaggccc accatcagtc cggatttggt cggcgtgtcc
     1441 ggatgcagtt ccttgaagcg cttgagcagg ctcagcgaat acgcatagtc cgagccgggg
     1501 cgtgcctcct tgtacaggcg gggcacggtt tcgaggttgt ggttcatcac gtccggcggt
     1561 gcggccttca ggatctccaa cgcacggtcc atgcggccgc ggaagtcggg ggtcaggatc
     1621 tcgatgcggg tgtgcggcga ctgttcgcgc acgcgccgga tgcactcggc aaagtgcgcg
     1681 gcgccgccgt cgcgcaggtc gtcgcggtcc accgaggtga tcaccacgta gctcagtttc
     1741 agcgcggcga tggttctggc caggttctcc ggttcttccg catcgagcgg atcggggcgg
     1801 ccgtggccga cgtcgcagaa cgggcagcgg cgcgtgcact tgtcacccat gatcatgaag
     1861 gtcgcggtgc ccttgccgaa gcattcgccg atgttcgggc agctggcttc ctcgcacacg
     1921 gtgaccagct tgtttgcgcg caggatgtcc ttgatctcgt agaagcgcgt cgacggcgac
     1981 gccgccttca cgcggatcca gtccggcttg gccagctttt ccgacggcac gatcttgatc
     2041 gggatgcggg cggtcttgcc ggcgcctttc tgcttttcgc tggggttgta ggcggtggag
     2101 gcaagaggct gtttgtcggt gctcatgatg gtcgtcgggg gcgcgggcgg gccgcggcgg
     2161 tcaggatttc aggtattcga tcagggtgcg ggccagcgcc tgctggactt cgtgcaaggg
     2221 gcgctccacg ccaagggtgc gcatgtccac ggtggccatc ccggcatagc cgcaagggtt
     2281 gatcccggaa aacggacgca ggtccattcc cacattcagc gatacgccat gataggtgca
     2341 gccgttgccg cggaccttca agcccagcgc ggcaattttc gcgcctttcc agcggccatc
     2401 gctgacgtag atgccgggcg cgccggcttt gcgctcgccg tccagattat acgtggccag
     2461 ggtgtcgatc acagcctgct cgatcttgtg cacgaactcg cgcgcgaaca gccgcttgcc
     2521 cgggcggcgg cgcaggtcca tcagcaggta gatcaccact tgccccggac cgtggtaagt
     2581 cacttccccg ccgcggtcgg tctgcacgac cggaatgccg ttggcatcga gcacatggga
     2641 tggatcggcg ccgagcccca gcgtgaatac cggcggatgc tcgacgatcc acagttcgtc
     2701 gcgggtgtcg gcgctgcgcg cgtcggtaaa cgcacgcatc gcggcatacg tggtctcgta
     2761 cggctcgtgg ccgcggtcga tcagggtcgg gagcatcgct cagaggggga tcgtgtagaa
     2821 gtggtgccgg tccttgtgag cggaattatc gatcactttg agaatcgtgc ggtcgaccgc
     2881 accttctgcc ggatcgaaca gtccgcagcg gcgcaaggcg gcgcgcacat cgatcgattt
     2941 tcccaggaac tcgaacacca cgcgggtgca ggcgatcgcg cgctgattgc tcgcggatac
     3001 gccgagcatc aggcccgaca ggcgcgcgac gcggttcttg taggtcggat acagcaccgt
     3061 gtgcgcgatc tcgttgccgg acagggattc gtaatccgtc aggaagatgc ggtcgttcag
     3121 aaagaaggtc gcgccgaggt acttgcagcg atacgtctgg tcaaggttgg cgtgctgggg
     3181 aaagcgctcg agccgctcgt gatacaccgc gccttcgtgg agaaaaatgc gcacgaggcc
     3241 gcgcagcact ttcccgggag cgctcatcga gtggtagtac tcgtagtagt agccgaggta
     3301 tttgtcgttc cgcgcggaag gctgttgctg caggcgcgcg atgtggtcca cgtaggcgag
     3361 atgttccgat cgctggcgcg gatgcacttg cacaacctgc ctgaactgtt gatgcgggag
     3421 atgtatctcc gatggttcca cgccgaagaa ttcgcagatg cgctccagcg tatggttgga
     3481 cggcctgctg gtgccgttca gatagcgatt gaactgggcg cggttgatgt tgagcttgcg
     3541 gcatacatcg gctacggaac ggtaatagct gcacagcatg cgcaggttct cgccgaattc
     3601 gcgctgggcg gtcagggtgc gggcagggag cgaacctggc atatttccac cggaaaacat
     3661 tgatgcgagg tgacgcgatt ctagcatcaa ctcgcatcaa cccgcgccaa ggagcgaaat
     3721 ggcgcgggct ggcgtttgcc cctaaagttc cgttcctatc cgtggccgct ttttcaatat
     3781 cttggaaaag cggctatggc ggggcataca agaacaagtc ggtccgcccc gacaacgcga
     3841 agcagccggc cacgaaccgg cgtgccgcat gaagacccaa aaggagacat gaatgcttcc
     3901 cttcgatgat tgcatcctgg gcaacagtgc gctgcatgcg cgctttctcg ccaccaccga
     3961 aatcctcgcc cgcctcgttt caagcgctcc ccgcaaggtt catctgactc agctcgagga
     4021 cgacaccggc cgttgcgcag ccgagatcac ggcgtgctgc gccgcgcttg ggcgcgcagg
     4081 gctcctcacc gtgcatgacg gtcacgaacc ggcatggtcg ctcgccggca cgcctgcgct
     4141 ggtcacactg gaagacgtgt tctgctgcgt gatgaacgag cagatgccgc gtgcgcgcaa
//
LOCUS       DATLGF010000226         7731 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_100723, whole genome shotgun sequence.
ACCESSION   DATLGF010000226 DATLGF010000000
VERSION     DATLGF010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 7731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7731
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100723"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="VM406_06830"
     CDS             <1..1074
                     /locus_tag="VM406_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014194131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HVL75713.1"
                     /translation="ECDRLGVRRPLLVTDKGVVAAGIVDKVLAQLSDPACAGVFDQTP
                     PNPHEAAVRAAAEQVREGRHDGIIAVGGGSSIDLAKGAAICATHTGPLKSFAVIEGGM
                     ARITSATAPVIAIPTTAGTGSEVGRGAILILEDGRKVGIISPHVVPKAAICDPELTMG
                     LPPMLTAATGMDAIAHCLETFMAPAFNPPADGIALDGLWRGWAHIERATRGPGDREAR
                     LNMMSASMQGAMAFQKGLGCVHSLSHSLGGINPRLHHGTLNAIFLPAVVTFNRDCTSM
                     RDEKKLDRIAHAMALPGGDAVAPALRAMSERLGLPTGLSELGVTRDLFPSIVDGALAD
                     HSHKTNPRTASAEDYMALLEQAF"
     gene            1141..1674
                     /locus_tag="VM406_06835"
     CDS             1141..1674
                     /locus_tag="VM406_06835"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HVL75714.1"
                     /translation="MARKGFGPHRVLEHLLVLGMSVMGLMVFGNVVLRYGFNSGLAIS
                     EEVSRFIFVWITFLGAIIALKDGVHLGVDTLVRRLPARAQLVCYWVSHLLMLWCCWLL
                     WSGSWVQTQLNWYNVAPISEIPVAAMYGSGLVAAALMALILLNNLRRSLVSLWTKGAA
                     GAAEHAARPGTAEGGAK"
     gene            1671..2948
                     /locus_tag="VM406_06840"
     CDS             1671..2948
                     /locus_tag="VM406_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HVL75715.1"
                     /translation="MTVFVFIGSLLGSMSLGMPIAFALLVCGIALMIFMGNLEAQILA
                     QKLIDGADSYPLMTIPFFLLAGELMNAGGLSRRIVAAAMSLFGHMRGGLGYVVIGTGL
                     LLASLSGSAIADTATLAVMLLPLMRQAGYNIGRSSGLIAATGIIAPVIPPSVGFVLFG
                     VTANLSITKLFMAGIVPGLLMALSLVVAWWWMSRSESFDLPEKKSGAEIRRALGDAVW
                     AIVLPIFIIAGLRFGIFTPTEAGVMAAVYALLVSVLVYRELSLEGLYEAVLNAAKTSA
                     VIMLLVAASAVPAWLITVANVPQQIIALLEPFMESPVLLMFVMVMFIIAIGMALDFTP
                     SILILTPVLMPVVHAAGIDPIYFGVMFIMTAAVGLLTPPVGAVLNTVCGVARIPMESA
                     VKGVLPFLIAEVLVLLLLVLFPSLVTVPASWFY"
     gene            2981..3967
                     /locus_tag="VM406_06845"
     CDS             2981..3967
                     /locus_tag="VM406_06845"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HVL75716.1"
                     /translation="MRIAKLIAMTLAAFAIQGASAQEVKLGYVPIEAHPVGQGVNRFI
                     ELVKTKSNGRINITPYSSGKLGNEPQMQSSLQGGFLEIMVGPTSNLVGSIKEFGIFDL
                     PFFYADFKAVDAVMDGRVGRQLFDKLDSINMVGLAYWDNGFRHMTNARRPVQKVEDIK
                     GLKIRVIPNPLFISTFRALGANPVPMPYPELYNALESKAVDAQETPVGLIEASKFYEV
                     QKYLTLSGHIYTPYVLLASKRWFSSLSDADKKLVLEAAAESAPYQRGLSRDNADRIAH
                     QVLKGHGMEVTTLAPAEHARMREMVRPVVDKFSDVIGADLIKQAREDMAKAR"
     gene            4053..5054
                     /gene="araD1"
                     /locus_tag="VM406_06850"
     CDS             4053..5054
                     /gene="araD1"
                     /locus_tag="VM406_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraD1 family protein"
                     /protein_id="HVL75717.1"
                     /translation="MRLIQFETVDGARAVGVVDGATIRQVNSVKTMRELALAAISAKR
                     SLSRQIEELGTGTSHDYDALLAENRVLPPLDHEDPAHCLVAGTGLTHIASAAARNQMH
                     QKLQAAEAEATLTDSMRMFKWGLEGGKPAGDEPGVQPEWFYKGDGSIVVRPGRAYPVP
                     SFADDEGEEPEVVGLYVIGDDGRPYRLGYAIGNEATDHVMEKKNYLYLPHSKLRYCSF
                     GPELRVGELPSHVQGTVRIRRGGEVIWEKPFATGEDNMCHALANLEYHHFKYNQFLRP
                     GDVHVQFFGTSVASFGDGVRTEDGDVFEIEIPEFGKPLINGTVRTPSKVGMRGVASL"
     gene            5147..6583
                     /locus_tag="VM406_06855"
     CDS             5147..6583
                     /locus_tag="VM406_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVL75718.1"
                     /translation="MTTYANHIGGEWVAGATTSRNINPADLSDVIGEFAQADEAQTHA
                     AIAAARQAFETWSLSTPQQRFDLLDRAGTSILARKDELGRLLAREEGKTLAEAVGEVA
                     RAGQIFKFFAGEALRLCGDTIPSVRSGLTVEVTREPLGVIGLITPWNFPIAIPAWKIA
                     PALAYGNCVVIKPADLVPASCWELVKIIVEAGAPRGVINLVMGRGSIVGEAIVASEDV
                     DAVSFTGSVGTGQMLAQKCVASGKKVQLEMGGKNPMVVLDDADLATAVEACINGAFFS
                     TGQRCTASSRLIVTEGIHDRFVEAMQARMKQLRVGNPLNPDTHIGPVVDDKQLKQDLD
                     YIDLAREEGASVFGGELIESAAKGYFLTPALATRTTNAMRINKEEVFGPFASVIKVKD
                     YDEALKVANETEFGLAAGICTTSLKHAQHFKRHAQAGMVMVNVPTAGVDYHVPFGGRK
                     GSSYGPREQGSYAREFYTTVKTAYTNAG"
     gene            complement(6623..6862)
                     /locus_tag="VM406_06860"
     CDS             complement(6623..6862)
                     /locus_tag="VM406_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HVL75719.1"
                     /translation="MKYENNSESRGPASLEPDSPCIGYCSTAFGADICAGCGRTAEEV
                     LQWIFMSEQEKRDIWTRIRSEGTAIRFKRQERQDP"
     gene            7000..7638
                     /locus_tag="VM406_06865"
     CDS             7000..7638
                     /locus_tag="VM406_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVL75720.1"
                     /translation="MRLHYYPGNANLAPHMLLEELGVPYELALVDRSVNAQKSAEYTK
                     LNPAGRIPTLVDGDLVLFESAAICLHLCDTHPDAGLAPPLGSAERAHFYKWLMYLTNT
                     VQTEMLFYFYPERLADDEAARAQVKRHAQERVADMFDLLERAYVEQGGPYFLGARFTA
                     VDCYLLMVSRWTRAMQRPAREREHLGPYLRRLLERPAIARAFESEGIQAPWI"
BASE COUNT         1337 a         2388 c         2535 g         1471 t
ORIGIN      
        1 gagtgcgatc gcctgggtgt gcgtcgtccg ctgctggtca ccgacaaggg cgtggtggcg
       61 gcaggcatcg tcgacaaggt tctcgcgcag ctgtccgacc ccgcatgcgc gggtgtgttc
      121 gatcagaccc cgcccaatcc gcacgaggct gccgtgcgcg ccgcagcgga gcaggttcgg
      181 gaagggcgcc atgacggcat catcgccgtc ggcggcggtt catcgatcga tctggcaaag
      241 ggcgcggcga tctgcgccac gcataccggt ccgctgaaaa gcttcgcggt gatcgaaggg
      301 ggcatggcgc gcatcacctc cgcaacggct ccggtgatcg ccatccctac aaccgcggga
      361 accggcagtg aagttgggcg cggcgcaatc ctgattctcg aagacggacg caaggtcggc
      421 atcatttcgc ctcacgtggt ccccaaggcg gccatctgcg atccggagct gacgatgggt
      481 cttccgccca tgctcaccgc cgcgaccggc atggatgcca ttgcgcactg cctcgaaacc
      541 ttcatggcgc cggcgttcaa tccgcccgcc gacggcatcg cgctcgacgg cctgtggcgc
      601 ggttgggcgc acatcgagcg cgccacgcgc ggtcccggcg accgcgaagc gaggctcaac
      661 atgatgagcg cctcgatgca gggggccatg gcgttccaga aaggcctggg atgcgtgcac
      721 agcctgagcc actcgcttgg tggcatcaac ccgcgcctgc accacggcac gctgaatgcg
      781 atcttcctgc cggcggttgt cacattcaat cgcgattgca cttcgatgcg cgacgaaaag
      841 aagctggatc gcatcgctca tgccatggca ctccccggtg gggatgccgt cgcaccggcg
      901 ctgcgcgcga tgagcgagcg gctcgggctt ccgaccggac tgtccgagct gggtgtcacg
      961 cgcgatctct ttccgagcat cgtggatggc gcgctcgctg accacagcca caagacgaat
     1021 ccgcgcaccg ccagcgcgga agactacatg gcgctgctcg aacaggcgtt ctgagagcgc
     1081 cttgccgtgc gcccttcgac aggcgcacgc ataaaaatca caagatcact ggagatatac
     1141 atggcaagga aaggctttgg gccgcaccgg gtcctcgagc atctgctcgt gctcggcatg
     1201 tccgtgatgg gactgatggt gttcggtaac gtcgtcctgc gctacggctt caactcgggt
     1261 ctggcgattt cggaagaggt gtcgcgcttc atctttgtgt ggatcacctt cctgggcgcg
     1321 atcatcgccc tcaaggatgg cgtgcatctt ggcgtcgata ccctcgtgcg caggttgccg
     1381 gcgcgcgcgc agctggtctg ttactgggtc agccatctgc taatgctgtg gtgctgctgg
     1441 ctgctctggt cgggcagctg ggtgcagacg cagctgaact ggtacaacgt cgcaccgatc
     1501 tcggaaattc cggtcgcggc catgtacggt tccggcctgg tcgccgcggc gttgatggcg
     1561 ctgatcctgc tgaataacct gcgccgctcg ctggtgtcgt tgtggaccaa gggcgctgcg
     1621 ggggcggcgg agcatgctgc gcgtcctggc actgcggaag ggggcgcgaa atgacggtat
     1681 tcgtattcat cggctccctg ctcggctcca tgagcctggg catgccgatc gcattcgcgt
     1741 tgctcgtctg cgggattgcg ctgatgatct tcatgggcaa cctcgaagcg cagatccttg
     1801 cccagaaact gatcgacggc gcggacagct atccgctgat gaccattccg ttcttcctgc
     1861 tcgccggcga gctgatgaat gcgggcggtc tgtcgcgtcg tatcgttgcc gccgcgatgt
     1921 ccctgtttgg ccatatgcgt gggggcctag gatatgtcgt gatcggcacc gggctgctgc
     1981 tcgccagcct gtccggctcg gccatcgccg atacggccac gctcgccgtc atgttgctgc
     2041 cgttgatgcg ccaggccggt tacaacatcg gccgttcctc cggccttatc gcagcgacgg
     2101 gcatcatcgc gccggtgatt ccgccttcgg tcggcttcgt gctgttcggc gtgacggcca
     2161 acctctcgat caccaagctg ttcatggccg ggatcgtgcc gggattgctg atggcgctgt
     2221 cgctggtcgt cgcctggtgg tggatgtcgc gcagcgagtc gttcgacctg cccgaaaaga
     2281 aatccggcgc cgaaatccgg cgtgcactgg gcgacgccgt ctgggccatc gtgctgccga
     2341 tattcatcat cgccggtctg cgcttcggca tcttcacgcc caccgaagcc ggggtcatgg
     2401 ccgctgtcta cgcgttgctg gtcagcgtgc tggtctaccg cgagctgtcg ctggagggct
     2461 tgtatgaggc ggtgctgaat gcggcgaaga cctcggccgt catcatgctg ctggtcgctg
     2521 cgtcggcggt gcccgcctgg ctgatcaccg tggccaatgt gccccagcag atcatcgcgc
     2581 tgctcgagcc cttcatggag tcgcctgtgc tgctcatgtt cgtgatggtg atgttcatca
     2641 tcgccatcgg catggcgctc gatttcacgc cgtcgatcct gatcctgacg ccggtgctga
     2701 tgcccgtggt ccacgctgcc ggcatcgacc cgatctactt cggggtgatg ttcatcatga
     2761 ccgcggccgt cggcctgctg acgcctccgg tgggggccgt cctgaacacc gtgtgcgggg
     2821 tggccaggat cccgatggag agcgccgtga agggcgtgct cccgttcctg attgccgagg
     2881 tgctggtgct gttgctgctc gttctgttcc cgagcctggt gacggtgccc gcatcctggt
     2941 tttactgaag caacaagaca tctttccaaa ggagacctca atgagaattg caaaactgat
     3001 cgccatgacg ctcgccgcct ttgccatcca gggcgcgtcc gcccaggagg tgaagctcgg
     3061 ttacgtgccc atcgaggcgc atccggtggg acagggcgtg aatcggttca tcgaactggt
     3121 gaagaccaag agcaatgggc gcatcaacat cacgccttac tcgagcggca agctgggtaa
     3181 tgaaccccag atgcagtcgt cgctgcaggg tggcttcctg gagatcatgg tcgggccgac
     3241 ctccaatctg gtcggatcga tcaaggagtt cgggattttc gaccttccgt tcttctacgc
     3301 tgatttcaag gccgtggatg cggtgatgga cgggcgtgtc ggccgccagc tgttcgacaa
     3361 gctcgattcg atcaacatgg tcggccttgc ctactgggat aacgggttcc gccacatgac
     3421 caacgcccgc cgtccggtgc agaaggtcga ggatatcaag ggcctgaaga tccgcgtgat
     3481 cccgaacccg ctgttcatct cgacgttccg cgcgctcggt gcgaatccgg ttccgatgcc
     3541 ttatccggag ctctacaacg cgctggaaag caaggctgtc gacgcgcagg aaacgccggt
     3601 cggcctgatc gaggccagca agttctatga agtgcagaag tacctgacgc tgagcggaca
     3661 catctatacg ccgtatgtcc tcctggcgag caagaggtgg ttcagcagcc tctccgacgc
     3721 cgacaagaag ctggttttgg aagcggccgc cgaatccgcg ccgtatcaac gcgggctctc
     3781 gcgcgacaac gccgaccgca tcgcgcacca ggtgctcaag gggcatggca tggaagtcac
     3841 cacgcttgct ccggccgagc acgcgcgcat gcgtgagatg gtcaggccgg tcgtggacaa
     3901 gttttccgac gtcatcggtg ccgacctgat caagcaggca cgcgaggaca tggccaaggc
     3961 gcgctaagtg cgccgcgcgg cgcacgggcg gcgccgcgcc tctcattcaa tgccgggttt
     4021 ccggcgccat tcatcttttg gagtagatcc tgatgcggtt aattcagttt gagacagtgg
     4081 acggtgcgcg cgccgtcggt gtggtggacg gggcgacgat tcgccaggtc aacagcgtga
     4141 agacaatgcg cgagctggcg cttgccgcca tctcggccaa gcgctcgctg tcgcggcaga
     4201 tcgaagagct gggaaccggt accagccatg attacgatgc gctgcttgcc gagaaccgcg
     4261 tcctgccgcc gctggatcac gaggatccgg cccactgcct cgtcgcgggt accggcctta
     4321 cccatatcgc cagtgccgcg gcacgcaatc aaatgcacca gaaactgcaa gcggccgagg
     4381 ccgaggccac gctgaccgac tccatgcgga tgttcaaatg gggactggag gggggcaagc
     4441 ccgcaggcga tgagccgggc gtgcagcccg agtggttcta caagggagac ggctccatcg
     4501 tcgtccgtcc ggggcgggct tatccggtgc cgtcgttcgc cgatgacgaa ggcgaagagc
     4561 ccgaagtggt gggcctgtac gtgatcggtg atgacgggcg tccgtaccgg ctcggctatg
     4621 cgatcggcaa tgaagcgacc gatcacgtga tggagaaaaa gaattatctg tatctcccgc
     4681 actcgaagct gcgctattgc tcgttcggcc cggagctgcg cgtgggcgaa ttgcccagcc
     4741 atgtccaggg caccgtgcgc attcgccgcg gtggcgaggt gatctgggag aagcccttcg
     4801 cgaccggcga ggacaacatg tgccatgcgc tcgccaacct cgaataccac cacttcaagt
     4861 acaaccagtt cctgcgcccg ggcgacgtgc atgtccagtt cttcggtacc tcggttgcgt
     4921 ctttcggcga cggtgtgcgc accgaggacg gcgatgtgtt cgagatcgag attcccgagt
     4981 tcggcaagcc gctgatcaac ggcacggttc gcacgccttc gaaggtcggc atgcgcggcg
     5041 tcgcatccct gtgatccgat aacaggcaga ggtgcttgcc tgctgccgcc tgcggtgcac
     5101 cctagcgcag gcggcgtcgt tctgcaaggc tttccaggac atcaccatga caacttacgc
     5161 aaaccatatc ggcggcgaat gggtggccgg tgccacgacc agccgcaaca tcaatccggc
     5221 cgatctctcc gatgtcatcg gcgagttcgc ccaggccgac gaggcacaaa cgcacgcggc
     5281 gatcgccgcc gcgcggcagg cgttcgagac gtggtcgctc tccacgccgc aacagcgctt
     5341 cgacctgctc gaccgtgccg gcacaagcat tcttgcgcgc aaggacgagc tgggaaggct
     5401 cctggctcgc gaggagggaa agacgctggc cgaagccgtc ggcgaggtcg cacgcgccgg
     5461 ccagatattc aagtttttcg cgggggaggc gctgcggctc tgcggcgata ccatcccttc
     5521 cgtgcggagc ggcctgaccg tcgaggtgac ccgcgagccg ctcggcgtga tcggtctcat
     5581 cacgccctgg aatttcccga ttgcgattcc ggcctggaag atcgcgcccg cactggccta
     5641 cggcaactgc gtggtcatca aaccggcgga tctcgttccg gcgagctgct gggagctggt
     5701 gaagatcatc gtggaagcag gggcgccgcg cggcgtcatc aaccttgtaa tgggacgcgg
     5761 atcgatagtc ggggaagcga tcgttgcatc ggaggacgtg gacgccgtga gcttcacggg
     5821 ctcggtcggt acgggccaaa tgctggcgca gaagtgcgtc gcatcgggca agaaggtgca
     5881 gctcgagatg ggtggcaaga acccgatggt cgtgctcgac gacgccgatc tcgcaaccgc
     5941 cgtggaggcc tgcatcaatg gcgcgttctt ctcgaccggc cagcgttgca ccgcgtcgtc
     6001 caggctgatt gttaccgagg gcatccatga ccgcttcgtc gaggccatgc aggcccgcat
     6061 gaagcagcta cgtgttggca atccgcttaa tcctgacact cacatcggcc cggtcgtcga
     6121 tgacaagcag ctcaagcagg accttgacta catcgatctc gcacgcgagg aaggggcgag
     6181 cgtgttcggg ggcgagctga tcgaatcggc cgccaagggg tacttcctca cgccggccct
     6241 ggcgacgcgc accaccaacg ccatgcgcat caacaaggag gaggttttcg gtccgttcgc
     6301 ttccgtcatc aaggtgaagg actatgacga ggcgctcaag gtagccaacg agaccgagtt
     6361 cgggcttgcg gcgggaatct gcaccacttc tctcaagcat gcccagcatt tcaagcggca
     6421 cgcgcaggcc ggcatggtga tggtgaatgt accaacggct ggcgtcgatt accacgtgcc
     6481 gttcggcggc aggaaaggtt cgagctacgg gccgcgtgag caagggagct acgcgaggga
     6541 gttctatacg acggtgaaga ccgcctatac gaacgccggc tgacctgtca ttcggcaagg
     6601 cgcattccgc ggaatgcgcc gcctatggat cctgccgttc ctgccgcttg aagcggatgg
     6661 ccgtgccttc cgagcgaatc cgggtccaga tgtcccgctt ttcctgctcg ctcatgaaaa
     6721 tccattgcag cacctcttcc gcggtgcgtc cgcagcccgc gcagatgtcc gcgccgaagg
     6781 ccgtcgagca gtagccgatg cacggcgaat ccggttccaa tgaggccggt ccccggctct
     6841 cgctgttgtt ctcatacttc atgtttcatt cctagtgcac cgcgcggtcg gcgcaatgcg
     6901 gccatacgca ttgttgcagg ttctgcataa tggcgcgacc gcagaagctg acatgatagc
     6961 cgtctcgtgg aaactgaaca gacaaggggg aagggagtga tgaggctgca ttactatccg
     7021 ggcaacgcca acctggcgcc gcacatgctg ctcgaggaat tgggcgtgcc ctatgaactg
     7081 gcgctggtcg atcgaagcgt caacgcgcag aagagcgcgg agtacacgaa gctcaatccg
     7141 gcgggccgga ttccgaccct cgtcgatggc gacctggtgc tgtttgaatc ggcggcgatc
     7201 tgcctgcacc tgtgcgacac ccatcccgac gcaggccttg cgccgccctt gggcagtgcc
     7261 gagcgtgcgc acttctacaa gtggctgatg tacctgacca atacggtaca gaccgagatg
     7321 ctgttctact tctatccgga gcggctggcg gacgacgagg ccgcgcgggc gcaggtgaag
     7381 cggcatgcgc aagagcgcgt cgcggacatg ttcgatctgc tggagcgggc gtatgtggag
     7441 cagggtgggc cgtactttct cggcgcgcgc ttcactgccg tggattgcta cctgctgatg
     7501 gtctcgcgct ggacgcgggc gatgcagcgc ccggcgcgcg agcgcgagca tctggggccg
     7561 tatctgcgcc ggctcctgga acggccggcg attgcgcgcg cgttcgagag cgaagggatt
     7621 caggcgccgt ggatctagat ctcggtggca gtgcgccggc cgatgcacgc tcgagggtgg
     7681 ggggccagtc accccgcctt cgccaatctc cgccacagcg tggtcctgct g
//
LOCUS       DATLGF010000227         4228 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_41929,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000227 DATLGF010000000
VERSION     DATLGF010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41929"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="VM406_06870"
     CDS             <1..1194
                     /locus_tag="VM406_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3394 domain-containing protein"
                     /protein_id="HVL75721.1"
                     /translation="FWATVFLIFVMLTQRPIQAYFRRRGNYFGAVRLGFTELIDGLIA
                     GARNMIGIGVATAAAGIIVGTVSLTGIGLVMTEVVELLSGGNLMLMLLLVALISLILG
                     MGLPTTANYIVVATLMAPVVVELGAQSGLIVPLIAVHLFVFYFGLMADVTPPVGLASF
                     AAAAIARTDPLRTGVTAFLYSMRTAILPFLFIFNTQLLMIGINSAWHLILTMGSALIA
                     MLVFAAATQGYFMVRNRWYETIALLLVTFTLFRPGFWWDQLYPPYEEAPGVQLLEFAD
                     TAPRNAQKRVWIEGDNLEGRTITKGVLLPLGEPGPARERLARAGLRVVPMGEELQILT
                     VQFGSQAARLGVEQGWRITRMEVPVQRPAKEWMFIPALLLLAAVIWWQRRRGSRQSKP
                     ARATA"
     gene            1341..2231
                     /locus_tag="VM406_06875"
     CDS             1341..2231
                     /locus_tag="VM406_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HVL75722.1"
                     /translation="MKPALRRIAAFAIPAALFAAPAQADTLAKIRASQAITIAHREAS
                     LPFSYLDANKKPVGYAVDLCHRIAEAVRRELKLPALQINYLLVTPSTRIPAIAEGKAD
                     LECGSTTNNAERRKEVAFTIPHFIAAARMVVRTDSGIRNWSDLRNKRVVTTKGTTTVK
                     LLNDRDAVRSLNLNLIEGKDHAESFDMVAANQAEAFPMDDVLLYGLRANSEKPESFTI
                     VGDALSAEPYAIMLRKDDPQFKALVDREMGRLILDGDIYKLYDKWFRAPIPPKGANMN
                     MPMGHLLRDTFRFPTDKVGD"
     gene            2355..3410
                     /gene="pyrC"
                     /locus_tag="VM406_06880"
     CDS             2355..3410
                     /gene="pyrC"
                     /locus_tag="VM406_06880"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HVL75723.1"
                     /translation="MTNPATSTPDSITLIRPDDWHLHLRDGAVLASVLPHTVRQFARA
                     IVMPNLRPPVTTTAQAAAYRERILAAVPAGERFEPLMTLYLTDNTPADEIRRARESGF
                     IHGVKLYPAGATTNSDAGVTSLSKCMKTLEVMQEVGMPLLVHGEVTHSDVDIFDREAV
                     FIEQVLAPLRRDLPGLKVVFEHITTREAAQYVAEADKNLAATITAHHLLYNRNEIFKG
                     GIRPHYYCLPVLKREQHRQALVQAATSGSPKFFLGTDSAPHPKGLKEHACGCAGCYTA
                     LHAMELYATAFEQAGALDKLEGFASRFGPAFYGLPLNAGTVTLRRESWQIPAELPMGE
                     ATVVPLDGGGTIGWRLV"
     gene            complement(3569..>4228)
                     /locus_tag="VM406_06885"
     CDS             complement(3569..>4228)
                     /locus_tag="VM406_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HVL75724.1"
                     /translation="LFDRAANMSLGGAWGTLSMGRMLSTGFHSIAAYELTGTANYGLI
                     GRQFGYTGGTRNNSEFRYTSPNFGGFTAALGYITSTDNASPDAEIGLNVIYSGGPIAA
                     GVGYTRLGAPVGGDDRDWSLGGSYNFGAFKVAASYHDPAGVSKGYQAGVGTTMGPVSL
                     VLDVVRETGTGIRHTDYLVEAKYSLSKRTTAYAVFTREGATGAALPRGNNFGVGLRHN
                     F"
BASE COUNT          755 a         1395 c         1353 g          725 t
ORIGIN      
        1 ttctgggcca ccgtgttcct gatcttcgtg atgctcaccc agcgcccgat ccaggcgtat
       61 ttccgccggc gcggcaacta cttcggcgcg gtccggctcg gctttaccga attgatcgac
      121 gggctgatcg ccggcgcgcg caacatgatc ggcatcggcg tagccaccgc cgcggccggc
      181 atcatcgtcg gcaccgtgtc gctgaccggc atcgggctgg tgatgacgga agtggtcgaa
      241 ctgctctccg gcggcaatct gatgctgatg ctcctgctag tagcgctgat cagcctgatc
      301 ctcgggatgg gcctgccaac cacggcgaac tacatcgtgg tggccacgct gatggcgccg
      361 gtggtggttg agctgggcgc gcaaagcggg ctgatcgtgc cgttgatcgc ggtgcacctg
      421 ttcgtgttct acttcgggct gatggccgac gtgacgccgc cggtcgggct ggcgtcgttc
      481 gcggcggcgg cgatcgcgcg caccgatccg ctcaggaccg gcgtgacggc cttcctgtac
      541 agcatgcgca ctgcgatcct gccgttcctg ttcatcttca acacccagct gctgatgatc
      601 gggatcaact cggcctggca cctgatcctg acgatgggca gcgcgctgat cgcaatgctg
      661 gtgttcgcgg ccgccacgca gggctacttc atggtgcgca accgctggta cgagacgatc
      721 gcgctgctgc tggtgacgtt tacgctgttc cggcccggct tctggtggga tcagctctac
      781 ccgccgtacg aggaagcgcc tggcgtgcaa ctgctcgaat tcgccgacac cgccccgcgc
      841 aacgcgcaaa agcgcgtctg gatcgaaggc gacaacctgg agggacgcac gatcaccaag
      901 ggcgtgctgc tgccgcttgg cgaacccggc ccggcacgcg agcggctcgc gcgcgccggc
      961 ctgcgcgtgg tgccgatggg cgaggagctg cagatcctca ctgtgcagtt cggctcccag
     1021 gccgcccgcc tcggcgtcga gcagggctgg cgcatcacca ggatggaagt accggtgcag
     1081 cgcccggcga aggagtggat gttcatcccc gccctgctgc tgctcgccgc ggtgatatgg
     1141 tggcagcgcc ggcgcggttc gcggcaatcc aagccggctc gcgcaacggc ctgatcaagt
     1201 gcgttatgca ttcttcgcat ccatcgatgc gaagaatgca ttggaacaag attgccgttt
     1261 tacaatagca tccgataccg ccctagatcg cggccgcctt acccgggcgg ccgcacgctg
     1321 cgtgtagagg gagacccagc atgaaacccg cactgcgccg tattgctgca tttgccattc
     1381 ccgctgcctt gttcgccgcg ccagcgcagg ccgacaccct ggcgaagatc cgagcctccc
     1441 aggcgatcac tatcgcccac cgcgaggcat cgctgccatt ttcctacctc gacgccaaca
     1501 agaagccggt cggctacgcc gtcgacctct gtcacaggat cgcagaggcg gtgcggcggg
     1561 agctgaagct gccggcgctg cagatcaact acctgctggt cacgcccagc acccgcattc
     1621 ccgcgatcgc ggaaggcaag gccgacctcg aatgcggttc gacgacgaac aacgccgagc
     1681 gccgcaagga agtcgccttc acgatccccc acttcatcgc cgctgcgcgc atggtggtgc
     1741 gcaccgattc cggcatccgc aactggtctg acctgcgcaa caagcgcgtc gtcaccacca
     1801 aggggacgac caccgtcaag ctgctcaacg accgtgacgc ggtgcggtcg ctgaacctga
     1861 atctcatcga gggcaaggac cacgccgagt ccttcgacat ggtcgccgcc aaccaggccg
     1921 aggccttccc gatggacgac gtgctgctgt acggcctgcg tgccaacagc gagaagccgg
     1981 aaagctttac catcgtcggc gatgcgctgt ctgccgagcc ttacgcgatc atgctgcgca
     2041 aggacgaccc gcagttcaag gcgctggtcg accgggagat ggggcgcctc atcctcgacg
     2101 gagacatcta caagctgtac gacaagtggt tccgcgcgcc catccccccg aagggggcga
     2161 acatgaacat gccgatgggg catctgctgc gcgacacgtt ccgtttcccg accgacaagg
     2221 tcggcgacta aggtccgtct cgatccggcg tcctcagcga ccctgggacg aaatccactc
     2281 cgcagcgtcc gcatccaagc ccgatcagtg gggcggggca ggtaaaatgg cgcgattcat
     2341 ctggaaccag cgccatgacc aacccagcca cttcaacccc tgactcgatc accctcatcc
     2401 gacccgacga ctggcacctg cacctgcgcg acggtgcggt cctcgccagc gtgctcccgc
     2461 acaccgtgcg ccagttcgcg cgtgccatcg tgatgcccaa cctgcgcccg ccggtgacca
     2521 ccaccgccca ggcggcggcc tatcgcgagc gcatcctcgc ggcagtgccg gccggcgagc
     2581 gcttcgaacc gctgatgacc ctgtacctga ctgacaacac gccggcagac gagatccgcc
     2641 gcgcgcgaga gagcgggttc atccatggcg tgaagctcta tccggcgggg gccaccacca
     2701 attcggacgc gggggtgact tcgctgtcga agtgcatgaa gacgctcgag gtaatgcagg
     2761 aggtcggcat gccgctgctc gtgcacggcg aggtcacgca ctcggacgtc gacatcttcg
     2821 atcgcgaggc ggtcttcatc gagcaggtgc tggcgccgtt gcggcgcgac ctgcccggcc
     2881 tgaaggtcgt gttcgagcac atcacgacgc gcgaagccgc gcagtatgtg gccgaagcgg
     2941 acaagaatct tgccgccacg atcactgcac atcatctgct ctacaaccgc aacgagattt
     3001 tcaagggcgg cattcgtccc cactactatt gtctgccggt gctcaagcgc gaacagcatc
     3061 gccaggcact ggtgcaggcg gccacctccg gcagccccaa gttcttcctc ggaaccgatt
     3121 cggcgccaca tccgaagggg ttgaaggaac atgcctgcgg ctgcgccggc tgctacacgg
     3181 ccctgcacgc aatggagctg tacgcgaccg ccttcgagca ggcgggtgcg ctggacaagc
     3241 tggagggttt cgccagccgg ttcggcccgg cgttctatgg ccttccgctc aacgccggca
     3301 ccgtgaccct gcgccgcgaa agctggcaaa ttccggcaga actgccgatg ggcgaggcca
     3361 ctgtggtccc gctcgatgga ggcggaacga tcggatggcg cttggtgtaa acctgggtta
     3421 ggccagcccg tcctgggcca tccgggacgg cgcagggctt gcggcgcagg ttagcaatcc
     3481 aaacaaaagg cgcatccaat aaaaaacccg caaccgaagt tgcgggtttt ttgttcttca
     3541 actccggctg gcttatgcca gcgcagaatt agaagttgtg acgcaggccg acgccgaagt
     3601 tgttgccgcg cggcagtgct gcgccggtag cgccttcgcg ggtgaacacg gcgtaggcag
     3661 tggtacgctt gctcaggctg tacttggctt cgaccaggta gtcggtgtgg cggatgccgg
     3721 tgccggtttc acgcacaacg tcgagaacca ggctgaccgg gcccatcgtg gtgcccacgc
     3781 cggcttggta gcccttgctg acgccagccg gatcgtggta cgaagcggca accttgaagg
     3841 cgccgaagtt gtacgaacca cccagcgacc agtcgcggtc atcaccgccc accggggcgc
     3901 ccagacgggt gtagcccaca ccagcagcga tcgggccgcc cgagtagatc acgttcaggc
     3961 cgatttctgc gtcaggcgaa gcgttgtcgg tgctggtgat gtaaccgaga gcagccgtga
     4021 agccgccgaa gttcggggag gtgtaacgga actcgctgtt gttacgggtg ccgccggtgt
     4081 agccgaactg gcggccgatc aggccgtagt tggcggtgcc ggtcagttca taagcagcga
     4141 tggagtggaa gccggtggac agcatacggc ccatcgacag ggtaccccag gcgccgccca
     4201 gcgacatgtt ggctgcgcgg tcgaacag
//
LOCUS       DATLGF010000228         5392 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_116756, whole genome shotgun sequence.
ACCESSION   DATLGF010000228 DATLGF010000000
VERSION     DATLGF010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116756"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VM406_06890"
     CDS             <1..234
                     /locus_tag="VM406_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HVL75725.1"
                     /translation="ERTLIRDYMATVEGLLPKQTEKNLPQAVAIAAIPEEIRGFGHVK
                     ERHLKAAKEKEAKLLQEFGSPGAPAAGAKQHAA"
     gene            complement(618..3173)
                     /locus_tag="VM406_06895"
     CDS             complement(618..3173)
                     /locus_tag="VM406_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVL75726.1"
                     /translation="MNDSAKPHRHRAWSAAIFGLVFVAACAVGYVVVRDAVNKTVEIH
                     ALSVAEVVAAQAMTARSVYSAQIVEKLRRDGYGSNVDAHELPGFVPIPAQFLKLIGTN
                     ADADAALYRFRPVSKWNLEPEQGLSDDFLRWAWPQLEQQDRPNPEGPIEWAPVWRFES
                     QQDKGRVLRYLRADPAAQMACVSCHNSYEMRPAVQAMRTAAGISTGKQWQQHQLLGAL
                     AITIPLDNVEYVAAHQIRETAVLIFGILIASFAAIAWFSFRLARRDAALRDTVSQLQT
                     SEQQARHANALLLAKQGVERAFAELSTYLQAIDQHALVAVTDPNGRILQANDKFCRTS
                     GYGASQLVGSEHRIVKSGVHPDSFFTDLWATIASGEIWRGEICNRTADGSLYWVDSAI
                     VPLKDDAGRIARYISISIDISERKRAEQRMMFMATHDVLTGLANRNLLQDRISQAIAH
                     NRRSNRQAAVLFIDLDRFKTINDSLGHDVGDLLLIEVSRRLVSVVREEDTVARQGGDE
                     FIVLLASLDEGRCAAQVADKLLQAVAEPYLIKGKELHVSASIGIALFPDDGQSVDVLL
                     KHSDIAMYHAKEKGRNISQFFAAEMNVQAAERHEIGSELRQALARGELRLHYQPIVEM
                     RSGAVARMEVLLRWEHPRWGQVSPLKFIPLAEDNGLIIPISEWVLRTACDQLRAWREA
                     GFAVPRLSINLSARQFHQQDLLESVAAILAETGVDAAQIELEITEGMLMENTEQAVER
                     LRALNDMGFEIAVDDFGTGYSSLNYLKRFPIDTLKIDRSFVMDIASDPDDAAIVTAII
                     ALAHSLEMRVVAEGVEDEAQLAFLRAQGCDYFQGFYFSRPLPAADAEHVLRRC"
     gene            complement(3297..4016)
                     /gene="queC"
                     /locus_tag="VM406_06900"
     CDS             complement(3297..4016)
                     /gene="queC"
                     /locus_tag="VM406_06900"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HVL75727.1"
                     /translation="MNAATASAASRALVLFSGGQDSTTCLAWALERYAHVETIGFDYG
                     QRHAIELQVRPALLDKLRALSPQWAARLGEDHMIDLSLLGRISETALTKDMAIAMQDN
                     GLPNTFVPGRNLLFMTVAATLAYRRGLNVLVGGMCETDFSGYPDCRDDTMKALQVALN
                     LGMSTRLAIETPLMWLDKADTWRLAQQLGGAALVDLVRTETHTCYLGERGALHEWGHG
                     CGSCPACELRARGYQRFLLPT"
     gene            complement(4086..4829)
                     /locus_tag="VM406_06905"
     CDS             complement(4086..4829)
                     /locus_tag="VM406_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HVL75728.1"
                     /translation="MSHTIGALTLAALVALPAGAISAPPNAVKYRTSPPPSAELAYSI
                     KARQRGLVLEGSAMVRWKNAGRQFSVINETRSKLIGKILDTRSDGMIDEFGLAPLNFN
                     EKRFSKKPTATTFDRKQGTISFSSSEAVYPLTGGEQDRNSIVWQLVSVARAAPTQFKP
                     GSEWTFFVAGQRDAESWTFKVVKPERLSTPLGDLQTVQVRRLPPDGKGQQIDLWLAPQ
                     RDWYPVRLRFVDENGDYLEQTLERIARAS"
     gene            complement(4838..>5392)
                     /locus_tag="VM406_06910"
     CDS             complement(4838..>5392)
                     /locus_tag="VM406_06910"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HVL75729.1"
                     /translation="RITVLNFSSEGVINEYGVAPVLYSEKPWRRAMTNTHFQHDRQRI
                     SFSASTASYPYHGGEQDRASVLWQLAGIGRAAPERFEAGGELELIVAGTRDASPWRIQ
                     IIGLEEIDTALGKMTAWHLARERRSGSYDQRIDVWLAPDRHWYPVHVRYTQANGDSLD
                     LSLSRLTLTDGAPDAETNRMQSEE"
BASE COUNT          867 a         1737 c         1809 g          979 t
ORIGIN      
        1 gagcgcacgc tgatccgcga ctacatggcc acggtggaag gcctgctgcc gaagcagacc
       61 gagaagaacc tgccgcaggc ggtggcaatc gccgccatcc ccgaggagat ccgcggcttc
      121 ggtcatgtga aggagcgcca cctgaaggcc gccaaggaga aggaagcgaa gctgctgcag
      181 gagttcggat cgcccggcgc gccggccgcg ggggcgaagc agcacgcggc gtagcgggct
      241 tgttgggggg cttgaacgcc gctggactcc ggcttgcgcc ggagtgacgg gcgtttttgg
      301 gtcgtcgatc ttttttgatc cggctgcgcg ctttactcag ccgtcatccc tgcatgcgac
      361 tccacgcagc agacgaacga gattcctcgc gtcgctcgga atggcagcgt cgggtaccgt
      421 ccccaattgc cgtcgtcccg aacgcagtga gggcggcccg cgtcagggat ctcatcggca
      481 tggcacgcga cgccgcgcgg caagcggatg aagttcctcg cttcgctcgg agcgacggcc
      541 cttgccccgg gcatcgccag cgcacgcttc agtggctgcg gatctggttc gccagcatcc
      601 gagaacgacc gtcgttttca gcaccggcgc agcacgtgct cggcatcggc agccggcaga
      661 ggccggctga agtaaaagcc ctggaagtag tcgcagccct gcgcacgcag gaacgccagc
      721 tgcgcctcgt cttcgacgcc ttcggcgacc acgcgcatct caaggctgtg ggccagcgcg
      781 atgatggcgg tgacgatggc cgcatcgtcc ggatcgctgg cgatgtccat cacgaacgag
      841 cggtcgatct tcagggtgtc gatcgggaag cgcttcagat agttgaggct ggaatagccg
      901 gtgccgaaat cgtcgaccgc gatctcgaaa cccatgtcgt tcagtgcacg caatctttcc
      961 accgcctgct cggtgttctc catcagcatg ccttcggtaa tctccagctc gatctgggcg
     1021 gcgtccacgc cggtctcggc gagaatggcg gcaaccgatt cgagcagatc ctgctggtgg
     1081 aactgccgcg ccgacagatt gatcgacagg cgcgggaccg cgaagccggc ctcgcgccag
     1141 gcgcgcagct ggtcgcaggc ggtgcgcagc acccattcgc tgatcgggat gatcaggccg
     1201 ttgtcttcgg ccagagggat gaacttgagc ggcgagacct gtccccagcg cgggtgttcc
     1261 cagcgcagca gcacttccat ccgggcgacg gcgccgctcc tcatctcgac aatgggctgg
     1321 taatgcaggc gcagctcgcc gcgcgcgagc gcctggcgca actccgagcc gatctcgtgg
     1381 cgctcggccg cctgtacgtt catctcggcg gcgaagaact ggctgatgtt gcggcccttt
     1441 tccttcgcgt ggtacatcgc gatgtcgctg tgcttgagca gcacgtcgac gctctggccg
     1501 tcgtcgggga acagggcgat cccgatgctg gcgctcacat gcagttcctt gcccttgatc
     1561 aggtagggct cggccaccgc ctgcagcagc ttgtccgcga cctgcgcggc gcagcggcct
     1621 tcgtcgaggc tggccaggag cacgatgaat tcatcgccgc cctggcgcgc caccgtgtct
     1681 tcctcgcgca ccaccgagac cagccggcgg ctcacttcga tcagcagcag gtcgccgacg
     1741 tcgtgaccga gggaatcgtt gatcgtcttg aatcggtcca ggtcgatgaa cagcacggcg
     1801 gcctgtcggt tgctgcggcg gttgtgcgcg atcgcttgcg agatacggtc ctgcagcagg
     1861 ttgcggttgg ccaggccggt gagcacgtca tgcgtggcca tgaacatcat gcgctgctcg
     1921 gcgcgcttgc gctcgctgat gtcgatgctg atcgagatgt agcgcgcgat ccggccggcg
     1981 tcgtccttga gcggcacgat ggccgaatcg acccagtaca ggctgccatc ggcggttcgg
     2041 ttgcagatct cgccgcgcca gatctcgccg ctcgcgatgg tggcccacag gtcggtgaag
     2101 aagctgtccg gatgcacgcc ggacttcacg atcctgtgtt cgctgccgac cagctggctg
     2161 gcgccgtaac cgctggtcct gcagaacttg tcgttggcct gcaggatgcg cccgttcgga
     2221 tcggtcaccg ccaccagcgc atgctggtcg atcgcctgca ggtaggtcga cagctcggcg
     2281 aacgcgcgct cgacgccctg cttggccagc agcagggcat tggcatgccg cgcctgctgc
     2341 tcggaggtct gcagctggct gacggtgtcg cgcagcgccg catccctgcg ggcgaggcgg
     2401 aaactgaacc acgcgatcgc cgcgaagctg gcgatcagga tgccgaagat caggacggcg
     2461 gtctcgcgga tctggtgcgc ggcgacgtac tcgacgttgt cgagcgggat cgtgatcgcc
     2521 agtgcgccaa gcagctggtg ctgctgccac tgcttgccgg tcgatatgcc cgccgcggtg
     2581 cgcatggcct gcacggcggg gcgcatttcg tagctgttat ggcaggagac gcaggccatc
     2641 tgggcggcag ggtcggcgcg caggtagcgc agcacgcggc ccttgtcctg ctgcgattcg
     2701 aagcgccaca ccggtgccca ctcgatcgga ccttccgggt tcggacggtc ctgctgttcc
     2761 agctgcggcc aggcccagcg caggaaatcg tcgctcagcc cctgttccgg ttccaggttc
     2821 cacttgctga ccggacggaa gcgatacaac gcggcatcgg cgtcagcgtt cgtaccgatc
     2881 agtttgagaa attgcgcggg gatggggacg aaaccgggca gctcgtgcgc gtcgacattc
     2941 gagccatagc cgtcgcgcct gagcttttcg acaatttgcg ccgagtagac ggaccgcgcc
     3001 gtcatcgcct gcgcggcgac gacttcggcc acactcagcg cgtggatttc gacggtcttg
     3061 ttgacggcgt cgcgcacgac gacatagccg accgcgcatg ccgcaacaaa aaccagcccg
     3121 aaaatggcag cggaccacgc gcggtggcgg tgcggcttcg cggaatcgtt caaggccttt
     3181 tgtctcctgc catctctgcc gattgtcgtt atctagttaa cggcaggaaa cataatagga
     3241 caactcagag cccccgaaca acacgtgcga ccccgttcac ccacggcatc ggcacatcat
     3301 gttggcaaaa ggaaacgttg gtagccgcgc gcgcgcagtt cacaggccgg gcagctgccg
     3361 cagccatggc cccattcgtg cagcgcgccg cgctcgccca gatagcaggt gtgggtctcg
     3421 gtgcggacca ggtcgaccag cgcggcaccg ccgagctgct gtgccaggcg ccaggtgtcg
     3481 gccttgtcca gccacatcag cggcgtctcg atcgccaggc gggtggacat gccgaggttg
     3541 agcgcgacct gcagcgcctt catcgtgtcg tcgcggcagt ccggatagcc ggaaaagtcg
     3601 gtttcgcaca tgccgccgac cagaacgttg agtccgcgcc ggtaagcgag cgtggcggcg
     3661 accgtcatga acagcaggtt gcgcccgggg acgaaggtgt tgggcaggcc gttgtcctgc
     3721 attgcgatcg ccatgtcctt ggtgagcgcg gtttcggaga tcctgccgag cagggacagg
     3781 tcgatcatgt ggtcctcgcc caggcgcgcc gcccactgcg gcgacagcgc gcgcagtttg
     3841 tccagcagcg ccgggcgcac ctgcagttcg atcgcgtggc gctgcccgta gtcgaatcct
     3901 atggtttcga catgtgcata gcgctccagc gcccaggcca ggcaggtggt ggaatcctgc
     3961 ccgccgctga acaggacgag ggcacggctg gcggcggatg cggtggcggc gttcatgact
     4021 ggtagcgaag cggcaagcgg agcaaaaggg gagggattgt agcgcgactt cccgctgcat
     4081 ccggctcagc tcgcgcgggc gatgcgctcc agcgtctgtt ccaggtaatc gccgttttcg
     4141 tcgacgaagc gcagtcgcac cgggtaccag tcgcgctgcg gcgccagcca caggtcgatc
     4201 tgctggccct tgccgtcggg cggcaggcgc cgcacctgca cagtctgcag gtcgcccagc
     4261 ggcgtactca ggcgttccgg cttgaccacc ttgaaggtcc agctttcggc gtcgcgctgg
     4321 ccggcgacga aaaaggtcca ttcgctgccc ggcttgaact gcgtcggcgc ggcgcgggca
     4381 actgacacca gctgccagac gatgctgttg cggtcctgtt cgccacccgt gagcgggtag
     4441 acggcctccg aggaagagaa gctgatcgtg ccctgcttgc ggtcgaaggt ggtggcggtg
     4501 ggtttcttgc tgaaacgctt ctcgttgaag ttcagcgggg caaggccgaa ctcgtcgatc
     4561 atgccgtcgc tgcgcgtgtc cagtatcttg ccgatcagct tcgagcgggt ttcgttgatg
     4621 accgagaact ggcgacccgc attcttccag cgcaccatcg cgctgccttc tagcaccagc
     4681 ccgcgctggc gcgccttgat gctgtaggcc agctccgccg agggcggggg gctggtccgg
     4741 tacttgaccg cgttcggcgg tgcgctgatc gcgccggccg gcagggcgac cagtgcggcc
     4801 agggtcagcg ccccgatggt gtgtgacaag cgtttgttca ttcttccgat tgcatcctgt
     4861 ttgtttcggc gtccggcgcg ccgtcggtca gcgtcaggcg cgagagcgac aggtccagac
     4921 tgtcgccgtt ggcctgggta tagcgcacat gcaccgggta ccagtgccga tccggcgcga
     4981 gccagacgtc gatgcgctgg tcgtagcttc cgctcctgcg ctcgcgcgcc aggtgccagg
     5041 ctgtcatttt gcccagcgcg gtgtcgattt cctccaggcc gatgatctgg atgcgccacg
     5101 gcgacgcgtc gcgcgtgccc gccacaatca gttccagttc gccgcctgcc tcaaaacgtt
     5161 ccggcgcagc ccggccgatc ccggccagct gccacagcac gctggcacgg tcctgctcac
     5221 cgccgtgata gggatagctt gcagtcgacg cggaaaaact gatgcgctgc ctgtcgtgct
     5281 ggaaatgcgt gttcgtcatc gcgcggcgcc acggcttttc actgtacagc accggcgcga
     5341 cgccgtactc attgatcacg ccctcgctgc tgaaattcag cacagtgatt ct
//
LOCUS       DATLGF010000229         6553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_149111, whole genome shotgun sequence.
ACCESSION   DATLGF010000229 DATLGF010000000
VERSION     DATLGF010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149111"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VM406_06915"
     CDS             complement(<1..590)
                     /locus_tag="VM406_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HVL75730.1"
                     /translation="MKSSEIREKFLKFFESKGHAIVASSPVVPGDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYTRATTSQKCIRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     HDAIQYAWELLTDVFKLPKEKLWVTVYAEDDEAYEIWNKVVGVPAERIVRIGDNKGAR
                     YASDNFWMMGDTGPCGPCTEIFYDHGPEIPGGPPGSP"
     gene            complement(691..1449)
                     /gene="ugpQ"
                     /locus_tag="VM406_06920"
     CDS             complement(691..1449)
                     /gene="ugpQ"
                     /locus_tag="VM406_06920"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HVL75731.1"
                     /translation="MWLYPRIVAHRGGGTLAPENTLAALRCGLAHGYRAVEFDVMLSS
                     DGVPILMHDPEFGRTVPGRGIVGELPAARLMALDAGSWFSPSFAGEPVPSFEQAARFC
                     KENGIWMNVEIKPVPGYEAATGKAVAEWTARLFPAEFADADRAALPLLSSFSPEALMA
                     ARQAAPKLPRALLVKSIPADWLARLRVLGAIALHVDHTRLTERRARAVKQHGFGLFCY
                     TVNSPGRARELLDWGVDAFCTDRIDLISPDPARL"
     gene            1576..4209
                     /locus_tag="VM406_06925"
     CDS             1576..4209
                     /locus_tag="VM406_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVL75732.1"
                     /translation="MPADSRQYSHPASNYPAPFDRANFLLPAEWQTLSDLLGANDTGL
                     LFIKDGVIAHANAGLCERLDTEVDDLLGKPVESLFPSGKEGAMTLEPVRGDRIRLLDS
                     HGTPVTFDLLESRVDTSSAAQVTIWVLQPAGHRLAPEKYTAEGTQAQAILDYLPDLVC
                     VCDSDTTLNYASRSLATELGYDGELQGLRLIDLAHPDDRGRLKAAIEPLKHADIGTLS
                     APFVVRVRHRDGSWRHIHAQARNLASHHAVAGILINGQDVTEQQQHQQNIAAEKKRQL
                     HYLNRLFRMAQRPHANLNPALNVILKSTARALGIHRCAYWEITEDPAASRCVLAYDDI
                     RQNFSEPELHPTFARHFHPLLYRVLHGEQAMAAGDVDQDPRTALHCEYFHAEEIKAAM
                     IVPVPDGDGAIGLLTLFHLREARNWRRDESEFVDNVARLISMIMHEVQRSRSEAQLRH
                     QAHHDSLTGLPNRQFLFEQAPDLLPQLTAGADTMAAFFIDLDGFKTVNDSLGHSVGDE
                     LLKAAALRLKNVVRKDDILVRLGGDEFMLLARNLSDMRIADDIAQQVVETMRNTFCLK
                     GRELQISASVGIAMYPQDGSDIETLMKKADLAMYQAKSAGRDQYQMFSARLDEASTDR
                     HTLEHELRRAIEERELRHYYQPQIDIRTGKVRCVEALLRWHHPEQGVLPPARFLPLAE
                     ETGLINTISAWALDDICQQLRTWEKAGMDGFRIAVNLSASQLMDRALLPALEQALERT
                     GIPGQKLEWEIKESTAMQHNTMASSLLDRVAELEVGLSIDDFGTGYSNMAYLRKYPVR
                     KVKIDGSLVGGLPDGRDHRAITDAIISMARPLGLDVVAEGVETPEQLEYLREHGCDIA
                     QGFYFSQPLTADQFEKWLIRH"
     gene            4191..5432
                     /locus_tag="VM406_06930"
     CDS             4191..5432
                     /locus_tag="VM406_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HVL75733.1"
                     /translation="MADSPLNNKALGHIRILDLTRVLAGPWCAQNLADLGADVIKIER
                     PGAGDDTRHWGPPYLKDSEGRDTSEAAYYLAANRGKRSVTLDIATPEGQDIVRRLARE
                     ADVVLENYKVGQLKKYGLDYESLKREKPDLVYCSITGFGQDGPYAQRAGYDFIIQGMG
                     GFMSITGERDDLPGGGPQKAGIAISDLMTGMYATIAVLAALAHRDRTGEGQYIDMALL
                     DVQVAMLANMATNYLASGNAPRRWGNAHPNIVPYQTFATSDGHIIVAVGNDGQYRRFV
                     EAGGRPELAVDERFATNPMRVRHRETLVPILAEMVQARTKQEWIDALEAAGVPCGPIN
                     TLDEVFENPQVVARGMRIELDHPSAGKVPLVRSPMKMSATPTTHEGAPPLLGQHTEEV
                     LCEVLGMSADEVKGLRARGVA"
     gene            5735..6025
                     /locus_tag="VM406_06935"
     CDS             5735..6025
                     /locus_tag="VM406_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HVL75734.1"
                     /translation="MNRCYYVYITASVSRVLYIGVTNDLVRRIREHKQKRIPGFTRQY
                     NVDRLVYFEQTTDVIAAISREKQLKGWRRDKKTALITEGNPHWDDLYSRTSA"
     gene            6056..>6553
                     /locus_tag="VM406_06940"
     CDS             6056..>6553
                     /locus_tag="VM406_06940"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HVL75735.1"
                     /translation="MIPFDWQNPYPTPRTPLFARNVVSTSHPLAAQAGLQMLFKGGNA
                     VDAAIAAAAVLTIVEPVSCGLGGDAFAIVWDGNALHGLNGSGPAPAAWTPKYFAKHGN
                     KMPQRGWDSVTVPGMVASWVELSERFGKLPFGKLLEPAIDLASRGYMVSPNVRQKWAA
                     AVPILK"
BASE COUNT         1250 a         2155 c         2089 g         1059 t
ORIGIN      
        1 ggcgagccgg gcgggccgcc ggggatctca gggccgtggt catagaagat ctcggtgcag
       61 gggccgcagg gaccggtgtc gcccatcatc cagaaattgt ccgacgcgta gcgcgcgccc
      121 ttgttgtcgc cgatgcgcac gatgcgctcg gccggcacgc caaccacctt gttccagatc
      181 tcgtaggcct cgtcgtcttc cgcgtagacg gtaacccaca gtttttcctt cggcagcttg
      241 aagacgtcgg tcagcagctc ccacgcgtac tggatcgcgt cgtgcttgaa gtagtcgccg
      301 aagctgaaat tgcccagcat ttcgaagaag gtatggtggc gcgcagtgta gccgacgttc
      361 tccaggtcgt tgtgcttgcc gccggcgcga atgcacttct gcgacgtggt ggcgcgcgtg
      421 taagggcgct tgtcgaagcc gaggaacacg tccttgaact ggttcatccc ggcgttggtg
      481 aacagcagcg tcgggtcgtc gcccggcacg acggggctgg aggcgacgat ggcgtgcccc
      541 ttggattcga agaacttcag gaacttctcg cggatttcgg acgatttcat gtgacagtgc
      601 ccagaaggac ggatggctgg atgcaaacgg ctgattatat atggaggcgg gcgggcgaag
      661 tggactgcac cggcgttgcc ggcgccgcat tcacagcctg gccggatcgg gactgatcag
      721 gtcgatacgg tccgtacaga aggcgtccac gccccagtcc agcagttcgc gggcgcggcc
      781 cgggctgttg acggtatagc aaaacaggcc gaagccgtgc tgcttgacgg cccgcgcgcg
      841 gcgctcggtg agccgggtgt gatccacatg cagcgcaatg gcacccagca cccgcaggcg
      901 ggccagccag tcggccggga tcgatttcac cagcaaggcg cgcggcagct tgggcgccgc
      961 ctggcgcgcc gccatcaggg cctccgggga gaacgacgac aggagcggca gcgctgccct
     1021 gtctgcatct gcgaactccg cagggaaaag acgcgcagtc cactccgcca ccgctttacc
     1081 tgtggctgcc tcgtagcctg gaaccggctt gatctcgacg ttcatccaga tgccgttttc
     1141 cttgcagaaa cgggccgcct gctcaaagct cgggaccggt tctcccgcaa acgaggggct
     1201 gaaccaggaa ccggcatcca gagccatgag cctggctgcc ggaagttcgc cgacgatgcc
     1261 gcgtccggga accgtgcggc cgaactcggg gtcatgcatc aatatcggga cgccgtcgct
     1321 gctgagcatg acgtcgaact cgacagcgcg ataaccatgg gcgagcccgc agcggagcgc
     1381 cgccagcgtg ttttcgggtg ccagcgtgcc gccgccgcga tgggcgacga tcctgggata
     1441 aagccacatc ggttgattta tgtcgtgtaa tgaaacgtcc cggctcgtag tctaaaccca
     1501 atctgttacc ccaacgacct gaaagcgaac cggggtccgc tgcaggcatg cgcagcgccc
     1561 ttctcgccgc cgcccatgcc tgctgacagc cgccaataca gtcaccccgc cagcaactac
     1621 ccggcccctt tcgaccgggc caacttcctt ctgcccgccg agtggcagac gctgtccgac
     1681 ctgctcggcg cgaacgatac cggccttctg ttcatcaagg acggcgtgat cgcccacgcc
     1741 aatgccggtc tgtgcgagcg gctggacacc gaggtcgacg acctgctggg caaaccggtc
     1801 gaatcattgt tcccaagcgg aaaggaaggc gcgatgacgc tggagccggt gcgcggcgac
     1861 cgcatccgcc tgctcgacag ccatggcacg ccggtgacct tcgacctgct cgaaagcagg
     1921 gtcgacacct cgtccgcggc gcaggtgacg atctgggtgt tgcagccggc ggggcaccgt
     1981 cttgcgccgg aaaaatacac ggctgaggga acgcaggccc aggccatact cgactacctg
     2041 cccgaccttg tgtgcgtctg cgattcagac accaccctca actacgccag ccgctcgctg
     2101 gccaccgaac tcggctacga cggcgagctg caggggctgc gcctgatcga tctcgcccat
     2161 ccggacgacc gagggcggct gaaggcggcg atcgagcctc tgaagcacgc cgacatcggc
     2221 acgctgagcg cgcctttcgt cgtgcgcgtg cgccacaggg acggctcctg gcgtcatatc
     2281 cacgcacagg cgcgcaacct cgcctcccat catgcggttg cgggcattct gatcaacggc
     2341 caggacgtga cggaacagca gcagcaccag cagaacatcg ccgcggagaa gaagcgccag
     2401 ctgcattacc tgaaccgctt gttccgcatg gcgcagcgcc ctcacgcgaa tctgaacccg
     2461 gcgttgaacg tgattctcaa gtccactgcg cgcgcgctcg gcatccatcg ctgcgcctac
     2521 tgggaaatca ccgaggatcc ggccgcctcg cgctgcgtgc ttgcctacga cgacatccgc
     2581 cagaactttt cggagccgga actgcatcca accttcgcaa ggcatttcca tccgctgctc
     2641 taccgagtgc tgcacggtga acaggcgatg gcagccggcg acgtcgacca ggacccgcgc
     2701 accgccctgc actgcgagta cttccatgcc gaggaaatca aggcggcgat gatcgtgccg
     2761 gttcccgacg gtgacggcgc gatcgggctg ctgacgctgt tccacctgcg cgaagcgcgc
     2821 aactggcgca gggacgagag cgagttcgtc gacaacgtcg cccgtctgat ctcgatgatc
     2881 atgcacgagg tgcagcgctc gcgcagcgaa gcgcagctgc ggcaccaggc gcaccatgac
     2941 agcctcaccg gcctgccgaa ccggcagttc ctgttcgagc aggcgccaga tctgctgccg
     3001 cagctgaccg cgggcgccga tacgatggcc gcctttttca tcgatctcga cggcttcaag
     3061 accgtgaacg actcgctcgg ccattcggtc ggggacgagc tgctcaaggc cgcagcgctg
     3121 cggctgaaga acgtggtgcg caaggacgac atcctggtgc gcctgggcgg cgatgagttc
     3181 atgctgctgg cccgcaatct gtcggacatg cgcatcgccg acgacatcgc ccagcaggtc
     3241 gtagaaacca tgcgcaacac cttctgcctg aagggccgcg agctgcagat ttcggcgtcg
     3301 gtgggaattg cgatgtatcc gcaggacgga agcgacatcg agaccctgat gaagaaggcc
     3361 gacctggcca tgtaccaggc caagtcggca gggcgcgacc agtaccagat gttttccgcg
     3421 cgtctggacg aagcaagtac cgaccgtcat acgctcgaac acgaactgcg ccgcgcgatc
     3481 gaggagcgcg aactgcgcca ctactaccag ccgcagatcg acatccgcac cggcaaggta
     3541 cgctgcgtgg aggcactgtt gcgctggcat cacccggaac agggtgtact gccgccggcg
     3601 cgctttcttc cgttggcgga ggaaaccggc ctgatcaaca cgatcagcgc ctgggcgctt
     3661 gacgacatct gccagcagct gcgcacgtgg gagaaagccg gcatggacgg tttccgcatc
     3721 gcggtcaacc tgtcggcgag ccagctgatg gaccgcgcgc tgctgcccgc tctggaacag
     3781 gcgctggagc gcaccggcat accggggcag aagctcgagt gggaaatcaa ggaaagcacc
     3841 gcgatgcagc acaacacgat ggcatcgtcg ctgctggacc gcgtggcgga actggaagtg
     3901 ggtttgtcga tcgacgattt cgggaccggc tattcgaaca tggcctacct gcggaagtat
     3961 ccggtgcgga aggttaaaat cgacggcagc cttgtcggcg gactccctga cgggcgcgac
     4021 catcgcgcca tcacggacgc catcatttcg atggccaggc cgctcgggct cgatgtcgtc
     4081 gcggagggcg tggaaacgcc ggagcagctc gaatacctgc gcgaacacgg ctgcgacatc
     4141 gctcagggtt tctacttctc ccaaccgctc accgcagacc aattcgaaaa atggctgatt
     4201 cgccactgaa caacaaagcc ctcggacaca tccgcatcct cgatctcacc cgcgtactgg
     4261 ccggcccctg gtgcgcccag aacctcgcag acctgggcgc ggacgtgatc aagatcgaac
     4321 gccccggcgc gggagacgac acccgccact gggggcctcc ttacctgaag gacagcgaag
     4381 gccgcgacac cagcgaagcg gcctattacc tcgccgcgaa ccgcggcaag cgctcggtga
     4441 cgctcgatat cgccacgccc gagggacagg acatcgtgcg ccggcttgcg cgcgaagccg
     4501 atgtcgtgct ggagaactac aaggtcgggc agctgaagaa atacggactg gactacgaat
     4561 cgctcaagcg ggagaagccc gatctcgtct actgctcgat caccggcttc gggcaggacg
     4621 gcccgtatgc ccagcgtgcc ggctacgact tcatcatcca gggcatgggc ggcttcatgt
     4681 cgatcaccgg cgagcgcgac gacctgcccg gcggcggacc gcagaaggcg gggatcgcga
     4741 tctcggacct gatgaccggc atgtacgcga ccatcgcggt gctggccgcg ctcgcgcatc
     4801 gcgaccgcac cggcgagggc cagtacatcg acatggccct gctcgatgtg caggtggcga
     4861 tgctggccaa catggcgacc aactacctgg ccagcggcaa cgcgccgcgg cgctggggca
     4921 atgcgcatcc gaacatcgtg ccttaccaga ccttcgccac gtcggacggc cacatcatcg
     4981 ttgcggtcgg caacgacggg caataccgcc gcttcgtcga ggcgggcggt cggccggagc
     5041 tggcggtcga tgaacgcttc gcgacgaatc cgatgcgtgt gcgccaccgc gaaacgctgg
     5101 tgcccatcct tgcggagatg gtgcaggcac gcacgaagca ggagtggatc gacgcgctgg
     5161 aagcggccgg cgtgccgtgc gggccgatca acacgctcga cgaagtcttc gagaatccgc
     5221 aggtcgttgc ccgcggcatg cgcatcgaac tcgatcatcc gtccgccggc aaggtgccgc
     5281 tggtcaggag cccgatgaag atgtcggcca cgccgaccac gcacgagggt gcgccgccct
     5341 tgctggggca gcataccgag gaggtgctct gcgaggtgct cgggatgagt gcggacgagg
     5401 tcaagggctt gagggcgcgc ggggtggcgt agggcttgga gcttacgctc ggtcgtctga
     5461 gagtctggtg gtctccaaga caatgaggtc cctcgctttg cccgggacga caaaattcct
     5521 cctgcctgtc gtcccgagca aagcgaggga cctcatccgc ttgccaagca acagcgctca
     5581 atgagtcaaa tgatttcaaa tttgtcgtcc cgagcgaagc gagggacctc atccgccaaa
     5641 acggcgccgt cccccaaacg caacttgcgc tagaatcatc cgctgtcatt ccggcaacac
     5701 cccctgtcgt tccgaatcag tgaggaaccc ggccatgaac cgctgctact acgtctacat
     5761 cacagccagc gtctcccggg tcctctacat cggcgtcacc aacgacctcg tgcgccgcat
     5821 ccgggagcac aagcagaagc gcatccccgg ctttaccagg cagtacaacg tcgaccggct
     5881 cgtctatttc gagcagacga ccgacgtcat cgccgcaatc agccgcgaga agcagctcaa
     5941 gggctggcgg cgcgacaaga aaacggccct gatcaccgaa ggcaacccgc actgggacga
     6001 cctgtattcc cgcaccagtg cctgaacgaa gcagtccaac cccaaggagc gccgcatgat
     6061 cccgttcgac tggcagaacc cctaccccac gccgcgcacg ccgctcttcg cgcgcaatgt
     6121 cgtgtccacc tcgcacccgc tcgccgcgca agccggcttg cagatgctgt tcaagggcgg
     6181 caacgccgtg gatgctgcaa tcgcggcggc cgccgtgctc actatcgtcg agccggtctc
     6241 ctgcgggctg ggcggcgatg catttgcgat cgtctgggac ggcaatgcgc tgcacgggct
     6301 gaacggctcg gggccggccc cggccgcgtg gacgccgaaa tacttcgcca agcacggcaa
     6361 caagatgccg cagcgcggct gggattccgt caccgtgccg ggcatggtcg caagctgggt
     6421 cgaactgtcg gagcgcttcg gcaagctgcc tttcggcaaa ctgctcgagc cggcgatcga
     6481 cctggcttcg cgcggctaca tggtctcgcc caacgtgcgg cagaaatggg cggccgcggt
     6541 gcccatcctg aag
//
LOCUS       DATLGF010000230         6712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_179964, whole genome shotgun sequence.
ACCESSION   DATLGF010000230 DATLGF010000000
VERSION     DATLGF010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6712
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179964"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="VM406_06945"
     CDS             complement(<1..964)
                     /locus_tag="VM406_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HVL75736.1"
                     /translation="MQIKISQQFDSGAIEVVRADHPSDIDLALRPDSHADVRQWFHFR
                     LQGAAGQPCRMRILNAAGAAFADGWHGYQACASYDRLNWFRVPTRYQDGVLTIEHAPE
                     RDSVYYAYFEPYSWERHLQLLGRVDGGRARVEDIGTTVDGRDLNMVVVGDPLARRKIW
                     VIARQHPGETMAEWFVEGMLDALLDRANPIARRLLQEAVLYVVPNMNPDGSVRGNLRT
                     NAAGANLNREWMAPSAERSPEVLAVRNRIHATGCDLFLDVHGDEALPYVFVAGCEMLE
                     GFTPAQAAAQQAFVDAFRRASPDFQTEHGYAPSKYREDLLQLASK"
     gene            1101..1177
                     /locus_tag="VM406_06950"
     tRNA            1101..1177
                     /locus_tag="VM406_06950"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1135..1137,aa:Pro,seq:ggg)
     gene            complement(1385..2224)
                     /locus_tag="VM406_06955"
     CDS             complement(1385..2224)
                     /locus_tag="VM406_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVL75737.1"
                     /translation="MAYKTILLHVDDSRNASARIALATQLALREEAHLIGAAWIGSSP
                     YVVESMMAGMDTPLIAGYLDELRQRAQEQLKRFEQAARDAGLASFSTRPVMNEPAGGL
                     SAAARYADLCILSQYDRDNASPSDSPDLPEHVVMRSGAPVLVVPAGWAASPFGQHLLV
                     AWNDSAEAWRAIHFALPLMKRAQAVDLVTLYKGELPAAPEERPGVRMAALLERHGIKT
                     ELIEKPAEGETGTALVALARQRGVDGIVMGCYGHSRVREVVLGGVTRRLLQDMHVPVL
                     MAH"
     gene            2434..3087
                     /locus_tag="VM406_06960"
     CDS             2434..3087
                     /locus_tag="VM406_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HVL75738.1"
                     /translation="MAPHRMFADRNDAAEALARQLSEYHGRKPLVLAIPRGAVPMGSI
                     LAERLQGELDLVLVRKLGSPIDPEYAIGAIDETGWVYIQPEAPDIMTSGPLLEQIKAD
                     ELAILKKRRADYTPFMPAIDAKDRVAIVVDDGLATGATMIAALHATRAREPAELVCAV
                     PVAATSSLEKVRPLADKVVCLYDTEYFGAVSLYYNRFTQVSDAEVAAILARRRPVPP"
     gene            3131..4183
                     /locus_tag="VM406_06965"
     CDS             3131..4183
                     /locus_tag="VM406_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012218271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75739.1"
                     /translation="MTHSPTNNSIPLERKVEFLRDPHAYGEAPDFTVGAIETHMSWVF
                     LTTRHAWKLKKPVWHEHLDFRSVAARHYYCEEELRLNRRLAPNVYLDVEPLCIDWRGH
                     LHLGPPGRPIDWLVKMQRLAERDMLDHAVRDLYVTRRDILRLARCLTQFYSTAEPAGI
                     SVAAYLAGLEREIDANLRDLCRSEYGLSAGHIERLCETQRAVLQERRSVFVARVEDGH
                     IVEGHGDLRPEHVCLHPQIAIIDCLEFSRELRLVDPIDELAFLTLELERQFASGHAAT
                     LMNAYYDVSGDRPHPLLACFYRSLRAAVRARLAIRHLDEERFRDVAEWPKRATAYLML
                     AQKYQDACNSLSDLPS"
     gene            complement(4144..5133)
                     /locus_tag="VM406_06970"
     CDS             complement(4144..5133)
                     /locus_tag="VM406_06970"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HVL75740.1"
                     /translation="MVAPSSMLVFALEASSGFGRRVAGHLGMELAPHEERRFEDGEHK
                     ARPLQSVRGRDVFVLHSLYGDGAASANDKLCTLLFFIGALNDAGAARVTAVAPYLAYA
                     RKDRRTKPRDPVTTRYVAALFEAVGADAVMTMDVHNLAAYQNAFRCLTVNLEARPLFI
                     AHCVKTLAGRDPVVVSPDAGGIKRAEAFREDLARALNVPVGMAFAEKYRSEGSVSGSM
                     LVGDVCGRDAIIVDDLISSGTTIARTAHACSKHGAGRVMALASHGLFAAGAAQALAEP
                     ALAQIVVTDTVPPFRLPAGPVRDKLTVLDSTALFAEAIRRLHEGRSLSELQAS"
     gene            5316..5705
                     /locus_tag="VM406_06975"
     CDS             5316..5705
                     /locus_tag="VM406_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVL75741.1"
                     /translation="MEQPVTSIMQRIVSPMSMDDSVAEVEAMMRAKHVSSAPVYDSDG
                     SILGIITSTDLVKFHSTGKDPTQVQAWEICTYRPIEVTPETPLSEVARLMLTHKVHHV
                     LVMKNEDLQGIVSSLDFVRLYLQQQPH"
     gene            complement(5707..>6712)
                     /locus_tag="VM406_06980"
     CDS             complement(5707..>6712)
                     /locus_tag="VM406_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HVL75742.1"
                     /translation="PYHRVETDLSTLPRTHTHLMVNLGNPDVAFRTSFLPADGVGLAR
                     MEFIVAEHIKVHPMALLHPERIDDEQVRAQIEALARGFERPADMFVRQLAEGVGTIAA
                     AFHPRPVIVRMSDFKTNEYASLLGGHWFEPHEENPMIGFRGAARYTHPAYAEGFALEC
                     AAMRRVREEMGLTNLKLMIPFCRRIVEARAVLEAMARQGLQRGENGLEIYVMCEIPNN
                     VVQVDAFAALFDGFSIGSNDLTQLVLGVDRDSEIVSFDFDERDEGVKIMLRQAVEGSR
                     RNGRHCGICGQAPSDYPEMAEYLVEIGIDSISLNPDTLIKTARRLLDVERRLGRPARH
                     "
BASE COUNT         1157 a         2244 c         2176 g         1135 t
ORIGIN      
        1 acttcgacgc cagttgcagc aggtcttcgc ggtacttgct cggcgcgtag ccatgctcgg
       61 tctggaaatc cgggctggcg cggcggaacg cgtcgacgaa ggcctgctgg gcggcggcct
      121 gcgccggcgt aaacccttcc agcatctcgc aaccggccac gaacacgtag ggcaaggctt
      181 catcgccatg cacgtccagg aacaggtcgc agccggtggc gtgaatacgg ttgcgcaccg
      241 ccaggacctc gggactgcgc tcggccgagg gtgccatcca ctcgcggttc aggtttgcgc
      301 cggccgcgtt ggtgcgcagg ttgccgcgca cgctgccgtc cggattcatg ttcggcacca
      361 cgtacagcac cgcctcctgc agcaggcggc gcgcaatcgg attggcccga tcgagcagcg
      421 catcgagcat gccctcgacg aaccattcgg ccatcgtctc gcccgggtgc tggcgcgcga
      481 tcacccagat cttcctgcgc gcgagcgggt cgccgaccac gaccatgttc aggtcgcgtc
      541 cgtccacggt ggtgccgatg tcctccacgc gcgcccggcc gccgtccacg cggccgagca
      601 gctgcaggtg gcgctcccag ctgtagggtt cgaaataggc gtaatagacg ctgtcacgtt
      661 ccggcgcatg ctcgatcgtc aacacgccgt cctggtagcg cgtgggcacg cggaaccagt
      721 tcaggcggtc gtagctggcg caggcctggt agccgtgcca gccgtcggca aaggcggcgc
      781 cggccgcatt caggatgcgc atccggcacg gctggccggc ggcgccctgc agccggaagt
      841 ggaaccactg gcgtacgtcg gcgtgcgaat cgggacggag cgcaaggtcg atatcggaag
      901 gatggtcggc gcggaccact tctatcgcac cgctgtcgaa ctgctggctg atcttgatct
      961 gcatggggaa ggcgaaacga gaatcctgcg cggatgtcgc gccggaaaag cagcattatg
     1021 gggtggaatg gcgcaggacg ctcgcgctca ggcgcgcgtg gcaatagttg cagcggctct
     1081 gctataatgt gcgctttcgt cggggcgtag cgcagcctgg tagcgcactt gcatggggtg
     1141 caaggggtcg gatgttcgaa tcatctcgcc ccgaccaaaa agtttcgaag gccaatcgca
     1201 agattggcct ttttgctttt ccgcgccgtt aaaacccatt ccgggctcgc gcagcaatat
     1261 ctgcattgtt tttcgtcaac cggaatttct ccttgcggct caatcacttg gcaaggctgc
     1321 cccaaccttg tccaccgaga tacccacagg agctgtgact aagtcgtccg gcgtcaggca
     1381 ggcgtcagtg cgccatcagc accggcacgt gcatgtcctg cagaagccgg cgcgtgaccc
     1441 cgcccagcac cacctcgcgt acgcgcgaat ggccatagca gcccatgacg atgccgtcga
     1501 ccccgcgctg gcgggcaagc gcgaccagcg cggtcccggt ttcgccttcg gccggcttct
     1561 cgatcagctc ggtcttgatg ccgtgccgct ccagcagcgc cgccatgcgc acgccggggc
     1621 gctcctcggg ggcggcgggg agttcgcctt tatatagcgt cacgaggtcg accgcctgcg
     1681 cgcgcttcat cagcggcagc gcgaaatgga tcgcgcgcca ggcttccgcg ctgtcgttcc
     1741 atgcgaccag caggtgctgg ccgaaggggc tcgcggccca gccggcggga acgaccagca
     1801 ccggcgcgcc gctgcgcatg accacatgct cgggcaggtc cgggctgtcc gacggagagg
     1861 cgttatcgcg gtcgtactgg ctcaggatgc acagatcggc gtagcgggcc gcggcgctca
     1921 agccaccggc cggctcgttc atgaccgggc gcgtggagaa tgaagcaagg ccggcgtcac
     1981 gcgccgcctg ctcgaaacgc ttaagctgtt cctgcgcgcg ctggcgcagt tcgtccaggt
     2041 agccggcgat caagggcgtg tccatgccgg ccatcatcga ctccacgaca tacggcgaac
     2101 tgccgatcca ggccgccccg atcagatggg cctcttcccg gagcgccagt tgcgtggcga
     2161 gcgcaatgcg ggcggacgcg tttctcgaat cgtccacgtg tagcaggatc gtcttgtaag
     2221 ccatgattcc tcctcggcat gcctggttga tgcgttgctc tatcagacta ggcgctgcaa
     2281 cgactgccgt ccatggcgcg cgcatcccct gacatgcctt ttcgctcaat cttgcatgcg
     2341 ctcaagcgaa ctgccgcgcg gcaagccgtt gcgggagcgt cgcgcaatga tctggcacaa
     2401 gccgccaccg tccagagcgc ctaccctgcc cgcatggccc cgcatcgcat gttcgccgac
     2461 cgcaacgacg ctgccgaggc gctggcgcgt cagctctccg aataccatgg ccgcaagccg
     2521 ctggtactgg cgattccgcg tggtgcagtt ccgatgggga gcatcctggc cgaacgcctg
     2581 cagggtgaac tcgaccttgt cctggtgcgc aagctcggct cgccgatcga tcccgaatac
     2641 gcgataggcg cgatcgacga gaccggctgg gtctacatcc agccggaagc gcctgacatc
     2701 atgaccagcg gaccgctgct ggaacagatc aaggcggacg aactggcgat cctgaaaaag
     2761 cggcgcgcag actatacccc tttcatgccg gcgatcgacg cgaaggaccg ggtcgcgatc
     2821 gtcgtcgacg acggactcgc caccggcgcc acgatgatcg cggcactgca tgcgacgcgc
     2881 gccagggaac ctgctgaact ggtctgcgcg gtgccggtcg cggcgacgtc cagcctcgag
     2941 aaggtcaggc cgctggcgga caaggtcgtg tgcctgtacg acaccgagta tttcggcgcc
     3001 gtcagcctct actataaccg tttcacccag gtgagcgacg ccgaggtggc ggccattctc
     3061 gcgcggcgac ggccagtgcc gccctgagcg gggcgactct gctcgggcac ctctgccgca
     3121 ccggtccgcg atgacgcatt caccgacgaa caacagcatc cctctcgaac gcaaggtcga
     3181 gttcctgcgc gatccgcacg cctacggcga agcgcccgat ttcaccgtgg gcgcgatcga
     3241 gacccacatg tcgtgggtgt tcctcaccac ccgacacgcc tggaagctca agaagccggt
     3301 ctggcacgag catctcgact ttcgcagcgt ggccgcgcgc cactattact gcgaggaaga
     3361 actacgcctg aaccggcgcc tggctccaaa cgtctacctc gacgtcgagc ccctgtgcat
     3421 cgactggcgc ggccatctgc acctcgggcc gccaggccgc ccgatcgact ggctggtgaa
     3481 gatgcagcgt ctggcagagc gcgacatgct cgaccacgcg gtgcgcgacc tgtacgtgac
     3541 gcgccgcgac atcctgcggc tggcacgctg ccttacccag ttctacagca ccgccgaacc
     3601 ggcgggcatc tcggtcgccg cctacctggc ggggctggag cgcgagatcg atgcgaacct
     3661 gcgcgacctg tgccgctcgg agtacgggct gagcgcaggc cacatcgaac gcctgtgcga
     3721 gacgcagcgc gcggtgctgc aggagcggcg ctcggtcttc gtggcacgcg tcgaggacgg
     3781 tcacatcgtc gaggggcatg gcgacctgcg cccggagcac gtgtgcctgc atccgcagat
     3841 cgcgatcatc gattgcctgg agttttcacg ggagctgcgc ctggtcgacc cgatcgacga
     3901 actggccttt ctcactctgg aactcgagcg gcagttcgcg tcggggcatg cggccacgct
     3961 gatgaacgcc tattacgatg tttccggcga ccgtccgcat ccgctgctcg cctgcttcta
     4021 tcggagcctg cgcgcggccg tgcgggcgcg cctggcgatc cgccacctcg atgaggaacg
     4081 ctttcgcgac gtggctgaat ggccgaagcg ggccactgcc tatctgatgc tcgcgcaaaa
     4141 atatcaggac gcctgcaatt cgctgagcga cctgccctcg tgaagacgcc ggatcgcttc
     4201 cgcgaacagc gccgtgctgt cgagaacggt cagtttgtcg cgcaccggtc cggcaggcag
     4261 gcgaaacggc gggaccgtgt cggtgacgac gatctgcgcg agcgccggct cggccagcgc
     4321 ctgcgctgcg cccgcagcga acaggccgtg actggccagt gccatcaccc gccctgcgcc
     4381 atgcttgctg cacgcatgcg cggtacgcgc aatcgtcgtt ccggaactga tcaagtcgtc
     4441 gacgatgatg gcgtcacgcc cgcagacgtc gccgaccagc atgctgccgc tgacgctgcc
     4501 ttcgctgcgg tatttctcgg caaacgccat gccgaccggc acattgagcg cgcgggcaag
     4561 atcctcgcga aatgcctccg ctcgcttgat gccgccggca tccggagaaa cgaccaccgg
     4621 atcgcgtccg gccaacgtct tcacgcaatg cgcgatgaac agcggccgcg cctccaggtt
     4681 cacggtcagg cagcgaaagg cgttctggta ggcggcgaga ttgtgcacat ccatcgtcat
     4741 caccgcgtcc gcgccgaccg cctcgaacag cgcggcgaca tagcgggtcg tgaccgggtc
     4801 gcgcggcttg gtcctgcggt ccttgcgggc ataggcgaga taaggcgcaa ccgccgtgac
     4861 gcgcgccgcg cccgcatcgt tcaaggcgcc gatgaagaag agcagcgtgc acagcttgtc
     4921 gtttgcgctt gccgcgccgt cgccgtagag agagtggagt acgaacacgt cgcggccgcg
     4981 cacgctctgc agcggccggg ccttgtgttc gccgtcctcg aagcggcgct cctcgtgcgg
     5041 ggcaagctcc atgcccagat gtccggcgac gcgccggccg aatccggaac ttgcttccag
     5101 ggcaaacacc agcatgctgg agggggcaac catcgaaatt ggatgcatgc gcggccggat
     5161 gtgtcccgcc ttctgccggc ggcgggcgcg gccttgagat gcatcaaacg cttccatgtg
     5221 gcgcgagccc ggcgtcgcgc cgatgggtat gctggcagca tgcaaacgcc gtgcaagcgt
     5281 ttcggcttgc gcggaaaacc tcagcaggcg aagcgatgga acagcccgtg acgtccatca
     5341 tgcagcgcat cgtcagtccg atgagcatgg acgacagcgt tgccgaggtg gaagcgatga
     5401 tgcgcgccaa gcatgtctcc tccgcgccgg tgtatgacag cgacggatcg attctcggca
     5461 tcatcacatc gaccgacctg gtgaagtttc actccaccgg caaggacccg acacaggtgc
     5521 aggcgtggga gatatgcacc taccgcccga tcgaggtcac gccggaaacg ccgctgtcgg
     5581 aggtcgcgcg cctgatgctc acgcacaagg tgcaccacgt gctggtgatg aagaacgagg
     5641 acctgcaggg catcgtctcg tcgctggact tcgtgcgcct gtatctgcag caacagccac
     5701 actgagtcag tgccgtgccg gccgtccgag ccgccgctcg acgtcgagca ggcgccgcgc
     5761 cgtcttgatc agcgtgtccg gattcaggct gatcgaatcg atgccgatct ccaccagata
     5821 ctcggccatt tccggatagt cggacggcgc ctggccgcag atgccgcaat gccggccgtt
     5881 gcggcgcgat ccttcgacgg cctggcgcag catgatcttc acgccttcgt cgcgttcgtc
     5941 gaaatcgaag gagacgatct cggagtcgcg atccacgccc agcaccagct gggtgagatc
     6001 gttggagccg atcgagaagc cgtcgaacag cgcggcgaag gcgtcgacct gcaccacgtt
     6061 gttcggaatc tcgcacatca catagatttc cagcccgttc tcgccgcgct gcaagccctg
     6121 ccgcgccatc gcctcgagca ccgcacgtgc ctccacaata cggcggcaga acggaatcat
     6181 cagcttcagg ttggtcagcc ccatctcctc gcggacgcgg cgcatcgccg cgcattcgag
     6241 cgcgaagccc tccgcatagg cgggatgggt gtagcgcgcc gccccgcgaa agccgatcat
     6301 cgggttttct tcatgcggct cgaaccagtg cccgccgagc aggcttgcgt attcattggt
     6361 cttgaaatcc gacatgcgca cgatcaccgg acgcggatga aaggctgcgg ctatcgtgcc
     6421 cacgccctct gcaagctggc gcacgaacat gtccgcgggc cgctcgaagc cgcgcgcgag
     6481 cgcctcgatc tgcgcgcgca cctgttcgtc atcgatgcgc tcgggatgca gcaaggccat
     6541 cggatgcacc ttgatgtgct cggcgacgat gaattccatg cgcgcaagcc cgacgccgtc
     6601 cgccggcagg aagctggtgc ggaacgccac gtccggattg ccgaggttga ccatcaggtg
     6661 cgtgtgggtg cgcggcagcg tggacagatc cgtttccacg cggtggtaag gc
//
LOCUS       DATLGF010000231         4434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_117069, whole genome shotgun sequence.
ACCESSION   DATLGF010000231 DATLGF010000000
VERSION     DATLGF010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117069"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..358)
                     /gene="rpsT"
                     /locus_tag="VM406_06985"
     CDS             complement(95..358)
                     /gene="rpsT"
                     /locus_tag="VM406_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HVL75743.1"
                     /translation="MANSAQARKRARQAVKHNAHNSSQRSTLRTAIKAVRKAIGLGDK
                     AAATQVFQSSVSIIDRIADKKIIHKNKAARHKSRLAAALKGLA"
     gene            516..2066
                     /gene="murJ"
                     /locus_tag="VM406_06990"
     CDS             516..2066
                     /gene="murJ"
                     /locus_tag="VM406_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HVL75744.1"
                     /translation="MNLHKTLATVSGMTMLSRVTGLVRELLIARAFGASAYTDAFFIA
                     FRIPNLLRRLFAEGAFSQAFVPILAEYKNQKGEEATRSLVDAVATVLMWALLATSAIG
                     VIGAPVIVLLVATGLHGEPEILGTSVTMTRIMFPYIALISFCALASGILNTWRSFQIP
                     AFTPVLLNLSFIAAALFVAPHLDQPVYALALAVVVGGILQVAIQIPALVRIGMLPRIR
                     LNPKTAYADPGVRRVMRQMGPAVFGVSVAQISLIINTNIASHLETGSVSWLSYADRLM
                     EFPTALLGVALGTILLPSLSKAHATGDQDEYSGLLDWGLRLTFLLALPAAIGLAALSE
                     ALTATLFHYGKFDANSVTMTGHALVAYGVGLIGLILVKILAPGFYAKQDIRTPVKIAV
                     GVLIATQMMNVLFVPVIAHAGLALSVGLGACINAAFLYAGLRRRAIYMPRAGWGRFFV
                     RLFVALAALGIVAWWSGAQFDWIAMQATPALRAGALAAILGVCMLTYFGMLFLLGFRF
                     RDFRRTSV"
     gene            2135..2971
                     /locus_tag="VM406_06995"
     CDS             2135..2971
                     /locus_tag="VM406_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVL75745.1"
                     /translation="MINPLDYFSSLVRQDDSIPLFEAALAIAQDADPQLDLSATQAEV
                     DVLAARLRRRLAADASNVQKLRLLNHFFYRELGFAGNINDYYNADNSYLQRVLRSRRG
                     IPISLAIVYMELAQQIGLNVKGVSFPGHFLMKMSVQSGDIVLDPFNGASLSREDLEER
                     LDPFLEQQAYPGAIPLAYYLHAAHPREILARMLRNLKALHLENLRWKEVLDVQQRLLI
                     LLPDEIVERRDRGLAYAHLECPQAALDDLEAYLAQRPHAEDAPALRKRLPELREASRR
                     LN"
     gene            complement(2964..>4434)
                     /locus_tag="VM406_07000"
     CDS             complement(2964..>4434)
                     /locus_tag="VM406_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVL75746.1"
                     /translation="ARMGTLSGGTQKRVALAAALVGEPDVLLLDEPTNHLDFTAILWL
                     EALLREFRGSIVFITHDRSFLDNVATRIVELDRGRLLSYPGNFSAYQKRKAEQLEVEA
                     IENAKFDKVLAQEEVWIRKGVEARRTRNEGRVKRLEELRRMRSARREQQGQVRLEVAS
                     GERSGKIVAELENVGKRYGDKVIVRDFSSIVLRGDKIGLIGPNGAGKTTLLKLILGEE
                     KPDSGTVRQGARLQVAYFDQMRAQLNEEASLVDTISPGSDWVEINGQRKHVMSYLGDF
                     LFAPERARSPVKSLSGGERNRLLLARLFAKPANVLVLDEPTNDLDIDTLELLEELLED
                     YDGTVFLVSHDRMFLDNVVTQVFVAEGAGQWREYIGGYSDWERVKASAPLPRPAAPPA
                     PVAPKGPAAQPAAPRESGRSKKLSYKEQRELESLPDLISQLEAEQKRISEQLADPELY
                     RTRPDEAQKLNQRFAEIDDELMASLEKWEAIEAKSRAAS"
BASE COUNT          723 a         1447 c         1350 g          914 t
ORIGIN      
        1 gcgaagcgct gaacgacaaa aacccgcgct gcgctgcaac gcgggttttc ttattcgtga
       61 caccacgcgc cgcgcgcggg tgaagcgact caggtcaggc caagcccttc agggcggcgg
      121 ccagacggct cttgtggcga gcagccttgt tcttgtggat gatcttcttg tcggcaatgc
      181 ggtcgatgat agacaccgag gattggaaaa cctgggtcgc cgccgccttg tcgccgaggc
      241 cgatcgcctt gcgcaccgcc ttgattgccg tgcgcagcgt cgagcgctgg ctggaattgt
      301 gagcgttatg cttgactgct tggcgagcgc gcttgcgcgc ttgtgcggaa tttgccatga
      361 aaaagttcct aaacgaaatc gggctgcgtt cgcggctgcg agacagaatt ctggaaagct
      421 ggcgagtata gcgttttttt gggaaaggag caagcgaccg tttcgccggc gcatgctgcc
      481 ggccatcaat accgctgccg ctataattgc ggcccatgaa tctccacaag accctcgcca
      541 cggtttccgg gatgaccatg ctctcccggg tcaccggcct ggtgcgagag cttctcatcg
      601 cgagggcgtt cggcgcttcc gcctacaccg acgccttctt catcgcattc cgcataccca
      661 acctgctgcg ccgcctgttc gccgaaggcg cgttttctca ggctttcgtg cccatcctcg
      721 ccgagtacaa gaaccagaaa ggcgaggagg ccacccgatc cctggtggac gcggtagcca
      781 ccgtcctcat gtgggcgctg ctcgcgacga gcgccatcgg cgtgatcggg gctccagtga
      841 ttgtcctgct ggtcgccacc ggcctgcatg gcgaacccga gattctcggc acctcggtga
      901 cgatgacgcg catcatgttt ccatacatcg cgctcatttc gttctgtgcg ctggccagcg
      961 gcattctcaa cacttggcgc agcttccaga ttcccgcgtt tacaccggtc ctgctcaacc
     1021 tctcgttcat cgcagccgca ctgttcgtcg ccccccacct cgaccaacct gtgtatgcgc
     1081 tggcgctggc agtggtggtc ggcgggattc tgcaggtggc gatccagatt ccggcgctcg
     1141 ttcgcatcgg catgctcccg cgcatacgcc tgaatccgaa gaccgcctat gccgatcccg
     1201 gcgtcaggcg agtcatgagg cagatgggac cggccgtctt cggcgtctcg gtcgcgcaga
     1261 tcagcctgat catcaatacc aatattgcct cccaccttga gacgggaagc gtgtcgtggc
     1321 tgtcgtatgc ggaccggctc atggagtttc ccaccgcgct gctgggcgtg gccttgggga
     1381 cgattctcct gcccagtctt tccaaggccc atgcgaccgg cgatcaggac gagtattccg
     1441 gcctgctcga ctggggactg cggttgactt tcctgctggc gctgccggct gcaatcgggc
     1501 tcgccgcgct ttccgaagcg ctgaccgcga ctcttttcca ctacggcaag ttcgatgcca
     1561 attccgttac gatgaccgga cacgcgctgg ttgcctacgg cgtcggactg atcggcctga
     1621 tcctcgtgaa gatcctggcg cctggattct atgcgaagca ggacatcagg acaccagtga
     1681 agatcgcggt cggggtgctg attgcaacgc agatgatgaa cgtgctgttc gtgcccgtca
     1741 tcgcgcatgc agggctggca ttgtcggtgg gtctcggcgc ctgcatcaat gccgcattcc
     1801 tgtacgccgg cttgcgccgc cgcgccatct acatgccgcg cgcgggctgg ggtcgattct
     1861 tcgttcgcct gttcgtcgcc ttggcggcgc tgggcatcgt cgcatggtgg agcggcgcgc
     1921 agttcgactg gatcgccatg caggccacgc cggcgctgcg tgccggagcc ctggcggcga
     1981 tccttggtgt ctgcatgctg acttatttcg gcatgctgtt cctgctgggt tttcgcttcc
     2041 gcgacttcag gcgcacctcg gtctgaggca ttgatcggat ccgcactttg caccctgccc
     2101 tttcattacg ggcgcgatgg tctaagatgc tagcgtgatc aatccgctcg attacttttc
     2161 ctctctggtc cgacaggacg attccattcc cctgttcgag gccgcgctcg ccattgccca
     2221 ggatgccgac ccgcagctcg acctatcggc aacgcaggcc gaggtcgacg tgctggcggc
     2281 gcgcttgcga cgccggctgg ccgcagacgc ctcgaacgta cagaagctgc gcctgctcaa
     2341 tcatttcttc tatcgagagc tggggttcgc aggcaacatc aacgactact acaacgcgga
     2401 caacagctat ctgcagcgcg tgctgcgcag ccggcgcggc atcccgatct cactcgccat
     2461 cgtgtacatg gaactggcgc agcagatcgg actgaacgtg aagggagtgt cctttcccgg
     2521 acatttcctg atgaagatgt cggtgcagtc gggagacatc gtgctcgatc cattcaatgg
     2581 agcaagcctc tcccgcgagg acctggaaga gcgcctcgac cctttcctgg aacagcaggc
     2641 ctatccggga gcgattcccc tggcctacta cctgcacgcc gcccatccgc gcgaaattct
     2701 cgcgcggatg ctgcgcaatc tgaaggcctt gcatctcgaa aacctgagat ggaaggaagt
     2761 gctcgacgtg cagcagcgcc tgctcatcct gcttcccgac gagattgtgg aacggcgcga
     2821 tcgcggtttg gcttacgccc acctcgaatg tccgcaggct gcgctggacg atctcgaagc
     2881 gtatctggcg cagcgcccgc acgcggagga tgcgccggcc ctgcgcaagc gcctccccga
     2941 actgcgtgaa gcgagccgcc ggctcaacta gcggcccgac ttttcgcttc gatcgcttcc
     3001 catttttcca ggctcgccat taattcgtcg tcgatctcgg caaagcgctg attcagcttc
     3061 tgcgcctcgt ccggccgcgt ccggtacaac tcggggtcgg caagctgctc ggagatgcgc
     3121 ttctgctcgg cctcgagctg ggaaatcaag tccggcaggc tttccagctc gcgttgctcc
     3181 ttgtagctca gcttcttcga cctgcccgat tcgcgcggcg cggcgggctg ggcggccggc
     3241 cccttcggtg cgaccggggc cggcggcgcg gcggggcgcg gcaggggagc cgatgccttg
     3301 acgcgctccc agtcgctgta gccgccgatg tactcgcgcc attgccccgc gccttcagcc
     3361 acgaacacct gagtaaccac attgtccagg aacatgcggt catggctgac caggaatacc
     3421 gtaccgtcgt agtcctcgag cagctcttcc agcaactcca gcgtgtcgat gtccaggtcg
     3481 ttggtcggtt cgtcgagcac cagcacgttc gccggcttgg caaacaggcg cgcaagcagc
     3541 aggcggttgc gttcgccacc ggagagcgat ttgaccggcg agcgcgcacg ttccggagcg
     3601 aacaggaaat cgccgaggta actcatcacg tgcttgcgct ggccgttgat ctcgacccag
     3661 tcactgccgg gcgagatcgt gtcgacgagg ctggcttcct cattcagctg cgcgcgcatc
     3721 tggtcgaaat acgcaacctg caggcgcgca ccctgacgca ccgtgccgga gtccggcttc
     3781 tcctcgccga gaatcagctt gagcaaggtg gtcttgccgg cgccgttagg gccgatcaac
     3841 ccgatcttgt cgccgcgcag cacgatgctg ctgaagtcac gcacgatgac cttgtcaccg
     3901 tagcgcttgc cgacgttctc cagttccgca acgattttcc cggagcgttc gccgctggcc
     3961 acttccaggc gcacctgtcc ctgctgctcg cgacgcgcac tgcgcatgcg gcgaagttcc
     4021 tcgagccgct tcacgcgccc ctcgttgcgg gtgcggcgcg cttccacgcc cttgcggatc
     4081 cacacttctt cctgcgccag gaccttgtcg aacttcgcgt tctcgattgc ctcgacctcg
     4141 agttgctccg ccttgcgctt ctggtaggcg gaaaaatttc cgggatagga gagcagtcgg
     4201 ccgcggtcca gctcgacgat gcgcgtggcg acgttgtcga ggaaggaacg gtcgtgcgtg
     4261 atgaagacga tgctgccgcg gaattcccgc aacagggctt cgagccacag gatcgccgtg
     4321 aaatcgaggt gattggtcgg ttcgtccagc aacagcacat ccggctcgcc gacgagggcc
     4381 gccgccagcg ccacgcgttt ctgcgtgccg ccggaaagcg tgcccatgcg cgcc
//
LOCUS       DATLGF010000232         8023 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_88781,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000232 DATLGF010000000
VERSION     DATLGF010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 8023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8023
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88781"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1861
                     /locus_tag="VM406_07005"
     CDS             <1..1861
                     /locus_tag="VM406_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVL75747.1"
                     /translation="RNRIMEIVPNPPSPAGRSGPRLRAAPAAQLEAPHVPLPNAALAP
                     PADIEPPTPLDRLIHAAMGRATGSISPAALALAYMDWAMHLAQAPGKWARLAEKAVRK
                     AARLAIYTACVQSNTRPGPCILPLPQDRRFRSAGWHEWPFNVIHQAFLLNQQWWHNAT
                     TGIGGVTLHHEQVVAFVTRQLLDLMAPTNFIFTNPDVLATTIEERGMNLLRGMNNLFE
                     DWERRVAGRPVAGTEQFQPGVQVAITPGKVVYRNRLIELIQYAPATDEVHAEPLLVVP
                     AWIMKYYILDLSPHNSLVRYLVERGHTVFMISWHNPDPRDADLGMEDYLHMGVLEALN
                     AVQAIVPGQRVNTVGYCLGGTLLAMAAAYLAQLGDTRLNSMTLLAAQTDFTEAGELRL
                     FIDDSQLNYLDDIMWSQGFLDNRQMAGAFQLLRAHDLIWSRMVQQYLMGQREVMSDLM
                     AWNVDATRMPYRMHTEYLHRLLLNNDLFAGRCKVNGRSISLGDIRVPIFAVATESDHV
                     APWQSVYKINLIADPDVTFLLTSGGHNAGIISEPGHPRRHYRMAKRLSGTNYVDPEQW
                     FYTTPAQEGSWWPEWSHWLERHGTGTAPPPPMGVPERGYSPLWPAPGQYVMEV"
     gene            2107..3144
                     /locus_tag="VM406_07010"
     CDS             2107..3144
                     /locus_tag="VM406_07010"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVL75748.1"
                     /translation="MEALLKHLVDITCQRDHALLDSSVVTALRELVGARQVRLHELTR
                     FRGDLYIRPRMWLDGKSVCTADEVLHGGQPGEPIGRYPKVVMAIGQRLNSVEETGADG
                     SHTLWVPIWLNDKVSSCLEIVKTGPCNRDTLHMLQGILGVYRNYQSILDYSERDSLTG
                     LLNRKTFDEKFSRLLTLSAPDDKPDDQEERRQDGKSGSSHWLGVLDIDHFKRVNDQFG
                     HLYGDEVLILIANLLRQSFRAQDRVFRFGGEEFVVLLRSAKLEDARRIFERFRANVEK
                     HDFPQVGRVTISLGFASISHETPVVILGHADQALYYAKANGRNRVCYYDELVQSGELR
                     SEVSNDTVEFF"
     gene            complement(3185..4129)
                     /gene="yedA"
                     /locus_tag="VM406_07015"
     CDS             complement(3185..4129)
                     /gene="yedA"
                     /locus_tag="VM406_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HVL75749.1"
                     /translation="MRTLPRLVLICLLTVYLVWGSTYLAIHFALESFPPFLLMGTRFL
                     VAGTLLYLFLCLRGTPQPSARQWRDGAVVGVLLLGGGMGLTAVAQQYVSSGMTAVFIA
                     SAPLMFALWAGLFGDWPGGRDWIGILAGFGGAALLASGAEFAAQPIGIVALVGAVSCW
                     TFGSVLSQKKLTVAPGPMGFASEMLAGGVFLVVLGLLRGEVLAGPFETRALLAWMYLI
                     TAGSLVAFSAYMYLLSKVSPALASSYAYVNPVIAVLLGVWLANEKVVAHELVAMAVIL
                     ASVILLTTSRRATRPLEKAGATASEQRECVGERSIEKA"
     gene            4249..4695
                     /locus_tag="VM406_07020"
     CDS             4249..4695
                     /locus_tag="VM406_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HVL75750.1"
                     /translation="MKIEFDTADVKILDILQADARTPLSEIGRRIHMSQPAVAERVRR
                     MEAAGVITGYHARVDPAALGYGITAFIRVAKRSHETPVEVVAERVPQIIECHSITGDD
                     CSIVKVVAKTVTELEQVITELTRCGVTSTSLILSSSVQRKGLRPAG"
     gene            4797..5741
                     /locus_tag="VM406_07025"
     CDS             4797..5741
                     /locus_tag="VM406_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVL75751.1"
                     /translation="MELRQLRYFVAIVDHGSLSRAARVLHVAQPALTQQIQQLEDELG
                     AQLLHRSAQGVLATDAGRIFYEHAQAILKQVGDAKSAVSQSTDRPAGTVTLGIPQSVS
                     GALALPLLNAVRETYPEISLQLTEELSGNLIEQLKSGRINLAVLFDDGQLTAFSTTAL
                     VEEQMMFITRAGSRFAPKKKSIALGQALATPLILPSLQHGVRPRIEKIASEAGLSIEN
                     VSDINSVAILRSALLADLGATILSVAPFLADIERGELCAWPISGSPISRTIALCSSRN
                     IPLTNAAAAVQRLVLAVTADLCDSGRWLGARRLTRQGA"
     gene            5934..>8023
                     /locus_tag="VM406_07030"
     CDS             5934..>8023
                     /locus_tag="VM406_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HVL75752.1"
                     /translation="MPAETAVIPAGPPAAASGGVVRSAPASSSPASSSPSLAPVKLDD
                     KYTSTSGKIFISGIQALVRLPIMQRQRDLAAGLHTAGFISGYRGSPLGGLDETLWKTQ
                     KLLEENHIQFVPGVNEDLAATAVWGTQTVDLIGPAKYDGVFAMWYGKGPGVDRCGDVF
                     KHMNHAGTSKHGGVLLVAGDDHGAYSSTLPHQSDHIFAACMIPVLYPCNVQEYLDLGL
                     HAWAMSRYSGCAVGFKALADTVESTASVDADPFRLQISYPDDFVMPEGGLNTRLSTDT
                     LGVQARKQEALMQDYKIYAALAYARANKLNRTTIDSPNARLGIIASGKSYMDVLEALE
                     ELGIDEAMAADIGIRLFKVSMPWPLEPDGVREFAQGLDEILVVEEKRQIVEYQLKEQL
                     YNWRDDVRPRVIGKFDEKGEWVHPRGDWLLPPKADFSIAQIARVIAQRIARLGLPTAT
                     SDLIKARLTFLEAKDQVLSKAVNTPPRPAWYCSGCPHNTSTKVPEGSLALAGIGCHVM
                     ATAIYPEHNKLTTHMGGEGAPWIGQAAFSKLPHVFQNLGDGTYFHSGYLAIRAAVAAR
                     VNMTYKILYNDAVAMTGGQPVDGTITVPMMAQQMAAEGVKRIALVTEDLSRYADRSAL
                     PAMVTLHDRKDMDAVQRELREVPGVSVLIYDQTCAAEKRRRRKKGEFPDPDKRLFINE
                     LVCEGCGDCGVQSN"
BASE COUNT         1466 a         2752 c         2548 g         1257 t
ORIGIN      
        1 caggaaccgc atcatggaga tcgtccccaa tcccccgtcg cccgccggac gcagcggccc
       61 gcggctgcgc gccgcccccg ccgcccagct ggaagcgccg catgtccccc tgcccaacgc
      121 ggcgctcgcg ccgcccgcgg acatcgagcc gccgaccccg ctcgaccgcc tgattcatgc
      181 cgcaatgggc cgggcaacgg gcagcatttc gccggcggcg ctcgcgctgg cctacatgga
      241 ctgggcgatg catctggcgc aagcgcccgg caagtgggca cggcttgcgg aaaaggctgt
      301 gcgcaaggcc gcccgccttg cgatctatac ggcctgcgtg cagagcaaca cgcgccccgg
      361 cccctgcatc ctgcccttgc cgcaggaccg gcgtttccgc agcgccggct ggcacgaatg
      421 gcctttcaac gtgatccacc aggctttcct gctgaaccag cagtggtggc acaacgcgac
      481 caccggcatt ggcggggtca cgctgcatca cgagcaggtg gtggcgttcg tcacgcgcca
      541 gctgctcgac ctgatggcgc cgaccaactt catcttcacc aatcccgatg tgctggccac
      601 gaccatcgag gagcgcggca tgaacctgct gcgcgggatg aacaacctgt tcgaggactg
      661 ggagcgccgc gtcgctggac gtccggtcgc cggtaccgaa cagttccagc ccggcgtgca
      721 ggtggcgatc accccgggca aggtggtcta ccgcaaccgc ctgatcgagc tgatccagta
      781 cgcgccggcg acggacgagg tgcacgccga gccgcttctg gtggtgccgg cctggatcat
      841 gaagtactac atcctcgacc tgtcgccgca caactcgctg gtgcgctatc tggtcgagcg
      901 cggccacacg gtgttcatga tctcctggca caaccccgat ccgcgcgatg ccgacctcgg
      961 catggaagac tatctgcaca tgggcgtgct tgaagcgctc aacgcggtgc aggccatcgt
     1021 tcccggccag cgcgtcaata ccgtgggcta ctgcctgggc ggcacgctgt tggcgatggc
     1081 ggccgcctat ctcgcccagc tcggtgacac gcgcctgaac tcgatgacgc tcttggccgc
     1141 gcagaccgac ttcaccgaag ccggcgaatt gcgcctgttc atcgacgaca gccagctgaa
     1201 ctacctcgac gacatcatgt ggagccaggg cttcctggac aaccgccaga tggccggcgc
     1261 cttccagctg ctgcgcgcgc acgacctgat ctggtcgcgc atggtccagc aatacctgat
     1321 ggggcagcgc gaggtgatga gcgacctgat ggcctggaac gtcgacgcca cgcgcatgcc
     1381 gtaccggatg cataccgaat acctgcaccg gctgctgctc aacaacgacc tgttcgccgg
     1441 ccgctgcaag gtcaatggcc ggtcgatctc gctgggtgac atccgtgtgc cgatcttcgc
     1501 ggtggcgacc gagagcgacc atgtcgctcc atggcagtcg gtctacaaga tcaacctgat
     1561 cgccgacccg gacgtgacct tcctgctgac ctccggcggc cacaatgcag gcatcatcag
     1621 cgaaccgggt cacccgaggc gccactaccg gatggccaag cgcctctcgg gcaccaacta
     1681 cgtcgatcct gaacaatggt tctatacgac gccggcgcag gaaggctcct ggtggccgga
     1741 gtggagccac tggctggaac gccacggcac cgggaccgcg ccgccgccgc cgatgggcgt
     1801 gccggagcgc ggctattctc cgctgtggcc ggcgcccggc cagtatgtga tggaagtgta
     1861 agcaggcggc aacatccgct ggcggccggc cggatttgct gtaacggcgc tttctttagg
     1921 gatagaatgc ttgcatcgca ccggccattg ccgccccgaa tcccgaggga caccgccggc
     1981 cgggtgccgc ggcggttcgc ctgtactgga atggagcagc gcccctgatg cataacccga
     2041 tctcggttca ccccgtcaag caggccccgc ttgaccgtca cccaggcagc ccgcgctcct
     2101 gacgcgatgg aagccctgct caagcacctg gtcgatatca cctgccagcg cgaccacgcg
     2161 ctgctcgaca gctccgtggt gacggcgctg cgcgagctgg tcggcgcgcg ccaggtgcgc
     2221 ctgcatgagc tgacgcgctt tcgcggagac ctctatatcc gcccccgcat gtggctcgac
     2281 ggcaaaagcg tatgcaccgc cgacgaagtg ctgcacggcg gacagccggg cgagccgatc
     2341 ggccgctatc cgaaggtcgt aatggcaatc ggccagcgcc tgaacagcgt agaagaaacc
     2401 ggcgcagacg gcagccacac gttatgggtg ccgatctggc tcaacgacaa ggtcagcagc
     2461 tgccttgaga tcgtcaagac cgggccctgc aaccgcgaca ccctgcacat gctgcagggc
     2521 attctcggcg tctaccgcaa ttaccagagc attctcgact acagcgagcg cgactcgctc
     2581 accggcctgc tgaaccgcaa gaccttcgac gagaaattct cgcgcctgct cacgctttcc
     2641 gcccccgacg acaagcccga cgaccaggag gagcgccgtc aggacggcaa gagcggcagc
     2701 agtcactggc tcggtgtgct cgacatcgat cacttcaagc gggtcaacga ccagttcggc
     2761 cacctgtacg gcgacgaagt cctgatcctg atcgccaacc tgctgcgcca gtcgttccgt
     2821 gcccaggacc gggtgttccg cttcggcggc gaggaattcg tcgtgctgct gcgctcggcc
     2881 aagctcgagg atgcgcgccg catcttcgag cgcttccgcg cgaacgtcga aaagcatgac
     2941 ttcccgcagg tggggcgagt gacgatcagc ctcggcttcg ccagcatttc ccacgaaacg
     3001 ccggtggtca tcctcggcca cgcggaccag gcgctgtact acgcgaaggc caacggccgc
     3061 aaccgcgtct gctactacga cgaactggtg caaagcggcg agctacgctc cgaagtgtcg
     3121 aacgacacgg tcgagttctt ctgatccgcc cgcgggtgcg gcgatgaagc gcgcccgccg
     3181 cggttcacgc tttctcgata ctcctctccc ctacacactc gcgctgctcg gaggccgtag
     3241 ctcctgcctt ctccagcggc cgggtcgcgc gccgcgaagt cgtcagcagg atcacgctgg
     3301 ccaggatcac ggccatcgcg accagttcat gcgcaacgac cttctcgttg gccagccaca
     3361 cgcccagcag caccgcgatc accggattga cgtaggcata gctcgaggcc agggccggcg
     3421 ataccttgga gagcagatac atgtaggcgg agaaggccac cagcgatccg gccgtgatca
     3481 gatacatcca ggccaggagc gcacgcgtct cgaacggccc ggccagcacc tcgccgcgca
     3541 gcaggccgag cacgaccagg aacacgccgc cggccagcat ctcgctggca aatcccatcg
     3601 ggccgggtgc gaccgtcagc ttcttctgcg acagcaccga gccgaaggtc cagcaggaga
     3661 ctgcgccgac gagcgcgacg atgccgatcg gctgtgccgc gaattccgca ccactggcca
     3721 ggagcgccgc cccgccaaaa ccggcgagga tgccgatcca gtcgcgcccg cccggccagt
     3781 cgccgaacag gccggcccac agcgcgaaca tcagcggcgc gctggcgatg aacaccgccg
     3841 tcatcccgga cgacacgtat tgctgggcca cagcggtcag ccccatgccg ccgcccagca
     3901 gcaggacgcc gaccaccgcg ccgtcgcgcc actggcgcgc gctcggctgc ggcgtgccgc
     3961 gcaggcagag aaacagatag agaagcgtgc cggccaccag gaagcgggtg cccatcagca
     4021 aaaaaggcgg aaaggattcg agcgcgaagt ggatcgccag ataggtcgag ccccagacca
     4081 ggtagacggt cagcaggcag atcagcacga gacggggaag ggtgcgcatg gtttttcttt
     4141 catcgttgtg gattgcagtg tagggggcgg caggcgcaaa ataaatgggg gtttgaagcc
     4201 gaaaatggga acaggctttg aacttcatga aaatgaaggg ggaatacctt gaagatcgaa
     4261 ttcgacacag ccgacgtcaa gattctcgac atcctgcagg cggacgcgcg caccccgcta
     4321 tccgaaatcg ggcggcgaat tcacatgagc cagcccgcgg tggccgagcg ggtgcgccgg
     4381 atggaagcgg cgggcgtgat caccggctac cacgcgcgcg tggatccggc ggcgctcggc
     4441 tacggcatca ccgccttcat ccgcgtggcc aagcgcagcc atgagacccc ggtcgaagtg
     4501 gtggccgagc gcgtgccgca gatcatcgaa tgccactcga tcaccggcga cgactgcagc
     4561 atcgtgaaag tggtggcgaa gacggtaacc gagctggagc aggtgatcac cgagctgacc
     4621 cgctgcggcg tgacctcgac ctcgctgatc ctgtcgtcgt cggtgcagcg caagggcttg
     4681 cggccggcgg gctaacgcgg aattgccttc cgcaccctgt cgtcctgagc cgcgggcgaa
     4741 ggacctcatc cgccgaccat cgcttgccgc cccggatcgc gcgctatcat cgccccatgg
     4801 aactgcgcca actccgctac ttcgtcgcca tcgtcgacca cggctcgctc tcgcgcgcgg
     4861 cgcgcgtgct gcacgtggcc cagccggcgc tgacgcagca gatccagcag ctcgaggacg
     4921 agctcggcgc gcagctgctg caccgctcgg cccagggcgt gctggccacc gacgccggcc
     4981 ggatcttcta cgagcatgcg caggcgatcc tgaagcaggt cggtgatgcg aagtcggcgg
     5041 tgtcgcaatc gaccgaccgc ccggctggca cggtcacgct gggcatcccg caaagcgtgt
     5101 ccggcgcgct ggcgctgcca ctgctcaacg cggtgcgcga gacctatccg gagatttcgc
     5161 tgcagctgac cgaggaactg tccggcaacc tgatcgagca gctgaaatcc ggccgcatca
     5221 acctcgcggt gctgttcgac gacggccagc tgaccgcgtt ctcgaccacc gcgctggtcg
     5281 aggaacagat gatgttcatc acccgcgccg gttcgcgctt tgcgccgaag aagaaatcga
     5341 tcgcgctggg ccaggcgctg gccacaccgc tgatcctgcc cagcctgcag cacggcgtgc
     5401 ggccgcgcat cgaaaagatc gccagcgaag ccgggctgtc gatcgagaac gtgagcgaca
     5461 tcaactcggt ggcgatcctg cgctccgcgc tgctggccga cctgggcgcg accatcctgt
     5521 cggtcgcccc gttcctggcc gacatcgagc gcggcgagct gtgcgcgtgg ccgatttccg
     5581 gcagcccgat ctcgcgcacg atcgcgctgt gctcgtcgcg caatattccg ctgaccaatg
     5641 ccgctgcggc ggtgcagcgt ctggtgctcg cggtgaccgc cgatctctgc gacagtggcc
     5701 gctggctggg tgcaagacga ctcacccgcc aaggggcata agtctgcctt atacccctat
     5761 cccgaatccc tatttgcccg cgcggcacga agcatcctaa actgcacggc atggaaataa
     5821 cggcaacgct attttcctga ggccgccacc ccggccatgc aacctggcac tcccctgcct
     5881 tacccggcag ccacctgtca ggcgctatcc gaaaaacagt tttggagaca tccatgccag
     5941 ccgaaacagc agtgatccct gctgggccgc ctgccgccgc atccggcggc gttgtgcgtt
     6001 ccgcgcccgc ttcttcctcc cccgcttctt cgtccccctc gctcgccccg gtcaagctcg
     6061 acgacaaata cacgtcgacc tccggcaaga tcttcatctc cggcatccag gctctggtgc
     6121 gcctgccgat catgcagcgc cagcgcgacc tcgccgccgg cctccatacc gccggcttca
     6181 tctccggcta tcgcggctcg ccgctcggcg ggctggacga gacgctgtgg aagacccaga
     6241 aactgctgga agaaaaccac atccagttcg tgccgggagt caatgaagac ctggccgcaa
     6301 ccgccgtctg gggcacgcag acggtcgatt tgatcggccc ggcgaagtac gacggcgtgt
     6361 tcgcgatgtg gtacggcaag ggccccggcg tggatcgctg cggcgacgtg ttcaagcaca
     6421 tgaaccatgc cggcacgtcg aagcatggcg gcgtgctgct ggtggccggc gacgaccacg
     6481 gcgcctattc ctccacgctg ccgcaccagt ccgaccacat cttcgccgcc tgcatgatcc
     6541 cggtgctgta tccgtgcaac gtgcaggaat acctggatct gggcctgcac gcctgggcga
     6601 tgtcgcgcta ctcgggctgc gcggtcggct tcaaggcgct tgccgacacg gtggaatcga
     6661 ccgcctccgt cgacgcggac ccgttccgcc tgcagatcag ctatccggac gatttcgtga
     6721 tgcccgaagg cggcctgaac acgcggctgt cgaccgacac gctgggcgtg caggcgcgca
     6781 agcaggaagc gctgatgcag gattacaaga tctatgcggc gctggcctat gcgcgggcga
     6841 acaagctcaa ccgcaccacg atcgacagcc cgaatgcgcg cctgggcatc atcgcgtcgg
     6901 gcaagtccta catggatgtg ctggaagcgc tggaggaact cggcatcgac gaggcgatgg
     6961 cggccgatat cggcatccgc ctgttcaagg tgtcgatgcc ctggccgctg gagccggacg
     7021 gcgtgcgcga attcgcgcag ggcctcgacg agattctggt cgtggaggaa aaacgccaga
     7081 tcgtcgaata ccagctgaag gagcagctgt acaactggcg cgatgacgtg cgcccgcgcg
     7141 tgatcggcaa gttcgacgag aagggcgaat gggtgcatcc gcgcggcgac tggctgctgc
     7201 cgccgaaagc ggacttttca atcgcgcaga tcgcacgcgt gatcgcgcag cgcatcgccc
     7261 gtctcggcct gccgactgcg accagcgacc tgatcaaggc gcgcctgact ttcctggaag
     7321 cgaaggacca ggtcctgtcc aaggcggtga acacgccgcc gcgtccggcc tggtactgct
     7381 ccggctgccc gcacaatacc tcgaccaagg tgccggaagg cagcctcgcg ctggccggca
     7441 tcggctgcca tgtgatggcc acggcgatct atccggaaca caacaagctg accacccaca
     7501 tgggcggcga aggcgcgccg tggatcggcc aggcggcatt ctcgaagctg ccgcatgtat
     7561 tccagaacct cggcgacggc acctatttcc actccggcta tctggcgatc cgggcggcgg
     7621 tggcggccag ggtcaacatg acctacaaga tcctgtacaa cgacgcggtc gcgatgaccg
     7681 gcggccagcc ggtcgacggc acgatcacgg tgccgatgat ggcccagcag atggccgccg
     7741 aaggcgtcaa gcgcatcgcg ctcgtcaccg aggacctgtc gcgctatgcg gatcgttccg
     7801 cgctgccggc gatggtgacg ctgcacgatc gcaaggacat ggacgccgtg cagcgcgaac
     7861 tgcgcgaggt gccgggcgtt tcggtgctga tctacgacca gacctgcgcg gccgagaagc
     7921 gccgccgccg caagaaaggc gagttccccg atcccgacaa gcggctgttc atcaatgaac
     7981 tggtgtgcga aggctgcggc gattgcggcg tgcagtcgaa ctg
//
LOCUS       DATLGF010000233         2172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_166961, whole genome shotgun sequence.
ACCESSION   DATLGF010000233 DATLGF010000000
VERSION     DATLGF010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166961"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2172)
                     /locus_tag="VM406_07035"
     CDS             complement(<1..>2172)
                     /locus_tag="VM406_07035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HVL75753.1"
                     /translation="TLSSWWMVERQHAQVVAELDRREADFHAATLSRSLYALSLRMAE
                     VAESTILATGLVDSAGKETYLTPYLTSIRQVNGVPIQILFADFEGKEISRNGDLHFDA
                     EQLAWMRKRLAEGSSGASVFDGPAGPELVAVELMHYPRTPSPEGAVLYKVMLADLLPA
                     PNVRVAWGGAPQQEDAHVVPVEVPPILAGLDLHLVEQRQERRLADPLRQYAIILVIAA
                     ALAALVFLIGSRLALALTAELRRLEAFSSSVIHHGFGSQRAEARGSDEVAGLAHSINH
                     MLDRLNEQHRLLQQETHKLHQLANTIPQLAWIARADGTIHWYNERWYAYTGSRPGEDS
                     DSLWTRTTPQAAAQLRRALAAGQAFHFTVPVRGADGEYRSFYTSAVPLHDGGGKLTQW
                     FGTSTDVSPLERAEQAVRDSEERLREGLAAARMAVWDWDLESGAMSFSANAAAVFGRE
                     LSDQAALWAAVHPDDHQALRAAMNAALAARSKFDKVVRVLDADGDLAWVEVRGRVVCD
                     ESGQPRAVRGISLDITQRKRAEDALLAANRRKDDFLAMLAHELRNPLAPISTAAQILQ
                     RIPVEPRVRDLSEIIARQTDHMNNLVDDLLDVSRVTRGLIKLEREVVRIDDIIAGAVE
                     QVRALIEVRRHELVLDVEVGEACVNADKTRLVQVVANLLNNAAKYTPDGGRIMLAATA
                     SGDEISIRVRDNGIGITPELLPQVFDLFIQAERSPDRSQGGL"
BASE COUNT          323 a          748 c          742 g          359 t
ORIGIN      
        1 cagccctccc tgcgagcggt cgggcgagcg ctcggcctgg atgaacagat cgaatacctg
       61 cggcagcagc tcgggcgtga tgccgatgcc gttgtcgcgc acgcggatgg agatctcgtc
      121 tccggatgcg gtggcggcaa gcatgatgcg tccgccatcg ggcgtgtact tggccgcgtt
      181 gttgagcagg ttggcgacca cctgcaccag gcgggtcttg tcggcgttca cgcaggcctc
      241 gcccacctcg acgtccagga ccagttcgtg gcggcgcacc tcgatcagcg cgcgtacctg
      301 ctccacggcg ccggcgatga tgtcatcgat gcgcaccacc tctcgttcga gcttgatcag
      361 cccgcgcgtc acgcgcgaga catcgagcag atcgtcgacc aggttgttca tgtgatcggt
      421 ctggcgcgca atgatctccg agagatcgcg cacccgcggc tcgaccggga tgcgctgcag
      481 gatctgggcg gcggtgctga tcggcgccag cggattgcgc agctcatgcg cgagcatcgc
      541 gaggaaatcg tccttgcgcc ggttcgccgc caggagcgca tcctcggcgc gcttgcgctg
      601 ggtgatgtcg agcgagatgc cgcgcaccgc gcgcggctgt ccgctttcgt cgcagacgac
      661 gcgaccgcgc acctcgaccc aggccaggtc tccgtcggcg tccagcacgc gcaccacctt
      721 gtcgaacttc gagcgggccg ccagcgccgc attcatcgcc gcgcgcaatg cctgatgatc
      781 gtccggatgc acggccgccc acaatgcggc ctggtcgctg agttcgcgcc cgaacacagc
      841 agcggcgttg gccgagaagc tcatcgcgcc gctttccagg tcccagtccc agaccgccat
      901 gcgcgccgcc gccaatcctt cgcgcagccg ttcttcgctg tcgcgcaccg cttgctcggc
      961 gcgctcgagc ggggagacgt cggtgctggt gccgaaccac tgcgtgagct tgccgccgcc
     1021 gtcgtgcaag ggcacggcgg aggtgtagaa agaccggtac tcgccgtcgg cgccgcggac
     1081 cggcaccgtg aagtgaaaag cctgtccggc cgcgagcgcc cggcgcagct gcgccgccgc
     1141 ctgcggcgtg gtgcgtgtcc acagggaatc gctgtcctcg ccggggcgac tgccggtgta
     1201 tgcataccag cgctcgttgt accagtggat ggtgccgtcc gcccgggcga tccaggccag
     1261 ctgcggaatg gtattggcga gctggtgcag cttgtgggtt tcctgctgca acaggcggtg
     1321 ctgctcgttg agcctgtcga gcatgtggtt gatcgagtgc gccaggcccg ccacttcgtc
     1381 gctgccgcgc gcctcggcgc gctggctgcc gaagccgtgg tggatcacgc tgctggagaa
     1441 ggcttccaga cggcgcagct ccgccgtcag cgccagggcc agccgcgagc cgatcagaaa
     1501 gaccagggcc gccagcgccg ccgcgatgac gaggatgatc gcgtactgcc gcaatgggtc
     1561 ggccaggcgc cgctcctgcc gctgctcgac caggtgcagg tccagtcccg ccaggatcgg
     1621 cggcacctcg accggtacca cgtgcgcatc ctcctgctgc ggcgcgccgc cccaggccac
     1681 gcgcacgttc ggcgccggca gcaggtcggc aagcatgacc ttgtacagca ccgcgccttc
     1741 cggcgagggc gtgcgcggat agtgcatcag ctcgacggcg accagctccg gtccggccgg
     1801 gccatcgaac accgaggcgc cggaacttcc ctcggccagg cgcttgcgca tccaggcaag
     1861 ctgctcggca tcgaaatgca gatcgccgtt gcgggagatt tccttgccct cgaaatcggc
     1921 aaacaggatc tggatcggca cgccgttgac ctgtcgaatg ctggtcaggt acggggtcag
     1981 gtaggtttcc ttgccggcgc tgtcgaccag gccggtggcg aggatcgtgc tctcggccac
     2041 ctcggccatg cgcaggctca gcgcatacag gctgcggctt agcgtggcgg catggaaatc
     2101 ggcctcgcgc cggtccagtt cggcgaccac ttgcgcatgc tgccgctcca ccatccacca
     2161 ggaggacagg gt
//
LOCUS       DATLGF010000234         5453 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_226897, whole genome shotgun sequence.
ACCESSION   DATLGF010000234 DATLGF010000000
VERSION     DATLGF010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226897"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..652)
                     /locus_tag="VM406_07040"
     CDS             complement(149..652)
                     /locus_tag="VM406_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HVL75754.1"
                     /translation="MTTIRSSLLALLLVPVLGAALADAHAQGAPHDNPLPPDFAVPVL
                     PEIKGVVSWNTLAKVKQVKSKDRILPEFSKEIAALNNQEVKIQGFMMPLEPGDRQKHF
                     LLSVSPQSCSFCLPAGPEGVVEVKSKTPVKYTFDPVIVSGKLSVLKDDPMGLYYRLTD
                     AVPASAK"
     gene            complement(812..1048)
                     /locus_tag="VM406_07045"
     CDS             complement(812..1048)
                     /locus_tag="VM406_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75755.1"
                     /translation="MKQSLALLLAAFALHAHAAKDDAHVKATIEQHRAIAEAHAQAAQ
                     CLESGKDEKTCHAELAKTCKGLAIGKLCGMRHKH"
     gene            complement(1063..2277)
                     /locus_tag="VM406_07050"
     CDS             complement(1063..2277)
                     /locus_tag="VM406_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL75756.1"
                     /translation="MKLGALTMALKSLRHRPLANAINMLLLAVGIAIMTFVLSAARQL
                     EDAALRDARGIDLVVGAPGSPLQLILSSIYHIDIPTGNIPLAAQATLEQNRLVRKVIP
                     LALGDSYEGFRIVGTTHDYAAHYGGQLAEGRQYQTEMEAVLGAAVAARTRLRPGEEFV
                     GSHGLAPGGETHEEAPYVVTGILQPTGTVLDRLVLTSVESVWHVHEIEHGITEDDAEG
                     KAAMEEDRELTALLVQYASPLAAATLPRLISSQSGLQAAQPAFESAKLFRMLGAGVDV
                     LRAIAAIILLAAALSMFAALYNALEERKTDLAILRTLGAPPGKLFRLLLAEGLLLAAG
                     GAALGWLTGHLALELVGRVLADGRSLPISGFAVAPDEIWLAAVALAIGLAAAALPALR
                     AYRTEIATTLSR"
     gene            complement(2274..2933)
                     /locus_tag="VM406_07055"
     CDS             complement(2274..2933)
                     /locus_tag="VM406_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVL75757.1"
                     /translation="MLQIHALSYRYPGARDAQLEVRDFSLESGRHAVLLGPSGSGKST
                     LLHLIAAILKPQQGRLIAAGTDLAALGPREADAWRGANVGFLPQKMALVPSLSARENI
                     LLAAYAPGKSQDRARADALLDALGIGDKASARPQQLSQGQQQRVALARALYHRPGLLL
                     ADEPTANLDDRACALAIELLVTQAAQAGASLVIATHDARVLAALPEAQVLRLSVPEAS
                     A"
     gene            complement(3078..3482)
                     /gene="dksA"
                     /locus_tag="VM406_07060"
     CDS             complement(3078..3482)
                     /gene="dksA"
                     /locus_tag="VM406_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HVL75758.1"
                     /translation="MLTEEQILQMSEKDYMNEAQLAFFKARLQQLERDLLKNAGETTE
                     HLRETVLVPDPADRATIEEEHALELRTRDRERKLLKKVQQAIASIDAGEYGWCEETGE
                     PIGIPRLLARPTATLSLEAQQRRELKQKLYGD"
     gene            complement(3558..3818)
                     /locus_tag="VM406_07065"
     CDS             complement(3558..3818)
                     /locus_tag="VM406_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75759.1"
                     /translation="MPKSIAKCAQSGVKSQVQSAAKKETGVPPSQAKTRRKEKQCMVF
                     PNLTEAKQARPCRARPCDAFQEASRPAMISSKRSGGSMDKIE"
     gene            complement(3848..4879)
                     /locus_tag="VM406_07070"
     CDS             complement(3848..4879)
                     /locus_tag="VM406_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HVL75760.1"
                     /translation="MALIPTTILTGFLGAGKTTLLNRILREPHGHRIAVIENEFGQEN
                     IDNEILVQDSNEQIVEMSNGCICCTVRGDLILALGALAQKRAAGEIDFDHVIIETTGL
                     ANPGPVAQTFFMDDEVALHYMLDAVVTVVDARHAMQQLDEHEEAQRQVGFADRILLSK
                     SDLVDDAQIERLQARLLRMNPRAPIVRADFGKVAVTEVLDLRGFNLNDKLELDPAFLA
                     AEEHAHEHVHDHACGEHCGHDHPHPHHSDEIAAFSFTSERPFDTARLDEFLGSLVRVY
                     GPRMLRYKGVLYMDGADRKVVFQGVHQTMGTDIGAKWADGPRMSKMVFIGKNLPQDVF
                     LQGLEQCLV"
     gene            complement(4892..5401)
                     /locus_tag="VM406_07075"
     CDS             complement(4892..5401)
                     /locus_tag="VM406_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HVL75761.1"
                     /translation="MSTRHPLPREPAPSDNEAKTHAARAQALLRDAAVRVTDARTRVL
                     CALLASPRALSHHDVQEAIADMDRVTLYRTLDCLTEAGLAHKISGDDRVFRYSAGAPH
                     AAGDSSPRLPHHQHGHFKCTRCAKVFCLDSNGEAALLRNAVQEILGRGFQSHDIEFTI
                     KGWCADCAR"
BASE COUNT          866 a         1707 c         1832 g         1048 t
ORIGIN      
        1 ctgaggaaga gagcgctcga tggcgggaga gcttcgtacc cgcgccgatt ctgcattaca
       61 acgacagtcg ggagctttgt caacgttgca aaagatttac cagttgcaac tggaggatgc
      121 gatatctccg caagtcggcg ctcccgcgct atttcgcaga ggctggcacg gcgtcggtca
      181 ggcggtagta cagacccatc ggatcgtcct tcagtacgct cagcttgccg gaaacaatca
      241 ccggatcgaa ggtgtacttg accggggttt tcgacttcac ttcgaccacg ccctcgggtc
      301 ccgccggcag gcaaaacgag cagctttgcg gcgataccga cagcaggaag tgcttctgcc
      361 gatcgcccgg ctccagcggc atcatgaagc cctggatctt gacctcctgg ttgttcagcg
      421 ctgcgatttc cttggaaaac tccggcagga tccggtcttt cgacttgacc tgcttgacct
      481 tggcaagcgt attccaggag accacgccct tgatttctgg cagcacggga accgcgaagt
      541 ccggcggcag cgggttgtcg tgcggagcgc cctgggcatg ggcgtcggca agcgcagcgc
      601 cgagcaccgg gaccagcagc agggcaagca gggaggaacg tatcgttgtc atgggcatcc
      661 ggcgtgaagg gtttcgcttg gaagccctga caaaaagccg cttgcggcgt tgcgccttct
      721 tgccatcggc attttgtcag ggcttcttat tctacgtggg ccgccggaac ggcggggacg
      781 agcgcttaga tggcgttccc cgctgcaagt ttcaatgctt gtggcgcatc ccgcacagtt
      841 tgccgattgc cagtcccttg caggtcttcg cgagctcggc gtggcaggtc ttctcgtcct
      901 tgcccgattc aaggcactgc gcggcctggg catgcgcttc ggcaatcgcg cggtgctgct
      961 cgatcgtggc cttcacatgc gcgtcgtcct ttgcggcatg ggcgtgcaat gcaaaagcgg
     1021 caagcagcag ggcgagggat tgtttcatct tgaaagctcc tttcaacgtg aaagcgtggt
     1081 ggcgatttcg gtgcggtatg cgcgcagtgc cggaagggcg gcggcggcca gtccgatggc
     1141 gagcgccacg gcagccagcc agatttcgtc cggcgcgacg gcgaatccgg agattggcaa
     1201 gctgcgcccg tcggcgagca cgcgaccgac cagctcgagc gcgagatgac cggtcagcca
     1261 tccgagcgct gctccgccag ctgccagcag caggccctcg gccagcagca ggcggaacag
     1321 cttgcccggc ggtgcaccaa gcgtgcgcag gattgcgagg tcggtcttgc gctcctccag
     1381 cgcgttataa agcgcagcga acatcgacag cgcggcagcc aacaggatga tggcggcgat
     1441 cgcgcgcagc acgtcgaccc cggcaccgag catgcggaac agcttggcgg attcgaatgc
     1501 cggttgtgca gcctgcaaac cgctctggct gctgatcaag cgcggcagcg tggcggccgc
     1561 cagcggggac gcgtactgca ccaggagggc ggtcagttcc cgatcctcct ccatcgccgc
     1621 cttgccctcg gcgtcgtcct cggtgatccc gtgctcgatt tcatgcacgt gccagaccga
     1681 ttcgacggat gtcagcacga ggcggtcaag cacggtgccg gtcggctgga ggatgcccgt
     1741 cacgacatac ggcgcctcct catgggtttc gccgccggga gccaggccgt gcgagccgac
     1801 gaattcctcg cctgggcgca agcgggtgcg ggccgcgacc gctgccccga gcaccgcttc
     1861 catctcggtc tgatattgcc ggccttcggc aagctggcca ccatagtggg cggcgtagtc
     1921 gtgcgtcgtg ccgacgatgc ggaacccctc gtaactgtcg ccgagcgcca gcgggatgac
     1981 cttgcgcacc agccggttct gctcgagcgt tgcctgggcc gccagcggga tgttcccggt
     2041 cggaatgtcg atgtgataga tcgatgagag gatcagctgc agcggcgagc cgggcgcccc
     2101 gaccacgagg tcgatgccgc gcgcgtcgcg cagcgccgca tcctctagct ggcgtgccgc
     2161 cgacagcacg aaggtcatga tcgcgatgcc gacggccagc agcagcatgt tgatcgcgtt
     2221 ggcgagcggc cggtggcgca acgacttcaa cgccattgtc agagcgccga gtttcatgcg
     2281 gacgcctccg gtaccgaaag tcgcaggacc tgtgcttcgg gaagggcggc aaggacgcgc
     2341 gcgtcatgag tcgcgatcac cagcgaggcg cccgcttgcg ccgcctgcgt caccagcagt
     2401 tcgatcgcga gcgcacacgc gcggtcgtcc agattggcgg tcggctcatc ggccagcagc
     2461 agccctggac ggtggtaaag cgcgcgagcc agggccacgc gttgctgctg cccctggctc
     2521 agctgttggg ggcgggcgct ggccttgtcg ccaatgccca gcgcgtcaag cagcgcgtcc
     2581 gcgcgtgccc ggtcctgcga cttgcccggc gcatacgcgg cgagcaggat gttttcccgt
     2641 gcactcaggc tgggcacgag cgccatcttc tgcggcagaa agccgacatt ggcaccgcgc
     2701 cacgcatccg cctcccgcgg gccgagagcg gccaggtcgg taccggcggc gatcagcctg
     2761 ccttgctgcg gtttcaaaat cgcggcaatc aagtggagca gcgtcgactt cccactgccc
     2821 gacgggccga gcagcaccgc gtgccgcccg gattcgagcg agaagtcgcg gacttcgagc
     2881 tgggcgtcgc gcgcgcccgg gtaacgataa gagagtgcgt ggatttgcaa catggttgca
     2941 ttatatcccg caagcgcgtc ctgcctgcaa gcagcatcgg tgcaaatccg gaggaatcgg
     3001 atgcgagcgg ccggctgaaa tgaaacaagc gcacgccggc tcgccggggt gcgcttgtgg
     3061 ggaggaggga acgagggtca gtcgccgtaa agcttttgct tgagctcgcg gcgctgctgc
     3121 gcttccagtg aaagggtggc ggtcggccgc gcgagcaggc gcggaatgcc gatcggttcg
     3181 ccggtttcct cgcaccagcc gtactcgccg gcatcgatgc tggcgatcgc ctgctggacc
     3241 ttcttcagca gcttgcgctc gcggtcgcgg gtgcgcaatt ccagcgcgtg ctcttcctcg
     3301 atcgtggcgc ggtcggccgg atcgggcacc agtaccgtct cgcgcaggtg ctccgtcgtt
     3361 tcgcccgcgt tcttcagcaa atcgcgttcg agctgctgca ggcgtgcctt gaaaaaggcc
     3421 agctgggcct cgttcatgta atccttctcg ctcatctgaa ggatttgctc ttccgtcaac
     3481 agggctccgt cggcgggagc ggcaggggtc ggtttggtcg tcttggtagc cacttcgcgt
     3541 actttcgaaa ctgcgggtta ctcgatttta tccatggatc cgccacttct tttgctgctg
     3601 atcatggcgg gcctggacgc ctcctggaac gcatcgcacg ggcgcgcccg gcagggtctc
     3661 gcctgctttg cctccgtcag gttcggaaag accatgcact gcttttcttt ccgtcgcgtt
     3721 ttggcttggc tcggcggtac gccggtttct ttcttggcgg cggattgtac ctgacttttt
     3781 accccgcttt gcgcgcattt ggcaatggat ttcggcattg ccgcgctgcc gggtcatgtc
     3841 gtgtgcacta gaccaggcac tgctccaggc cttgcaggaa cacgtcctgc ggcaggttct
     3901 tgccgatgaa taccatcttg ctcatgcgcg gcccgtccgc ccatttggcc ccgatgtcgg
     3961 tgcccatggt ctggtgcacc ccctggaaca ccactttccg gtcggcgccg tccatgtaca
     4021 gcacgccctt gtagcgcagc atgcgcgggc cgtacacgcg cacgaggctg cccaggaatt
     4081 cgtccaggcg cgcggtatcg aacgggcgtt cgctggtgaa ggagaacgcg gcaatctcgt
     4141 cgctgtggtg cgggtgagga tgatcatggc cgcagtgttc gccgcatgca tggtcgtgga
     4201 catgctcatg cgcgtgttct tcggccgcca ggaaggccgg atccagttcc agcttgtcgt
     4261 tcagattgaa gccgcgcagg tccagcacct cggtgacggc caccttgccg aaatcggcgc
     4321 gcacgatggg cgcgcgcggg ttcatgcgca gcaggcgcgc ctgcagccgt tcgatctgcg
     4381 cgtcgtcgac cagatcgctc ttggatagca ggatgcggtc ggcaaacccg acctggcgct
     4441 gggcttcctc gtgctcgtca agctgctgca tcgcgtggcg ggcgtcaacc accgtcacca
     4501 cggcatcgag catgtagtgg agagcgactt cgtcgtccat gaagaaggtc tgggcgaccg
     4561 gtccgggatt ggcaaggccg gtggtttcga tgatgacatg gtcgaagtcg atctctccgg
     4621 ccgcgcgctt ctgcgcgagc gccccaagcg ccaggatcag gtccccgcgc acggtgcagc
     4681 agatgcagcc gttgctcatc tcgacgatct gctcgttgct gtcctggacc aggatttcgt
     4741 tgtcgatgtt ttcctgcccg aactcgtttt cgatcaccgc gatcctgtgg ccgtgcggct
     4801 cgcgcaggat gcggttgagc agcgtggttt tgccggcgcc gagaaagccg gtcaggatgg
     4861 tagtcggtat aagtgccatg gcggtcggtc ctcaacgggc gcagtcggcg caccagccct
     4921 tgatggtgaa ttcgatgtcg tggctctgga agccgcgccc caggatttcc tggactgcat
     4981 tgcgcagcag cgccgcctcg ccgttgctgt cgaggcagaa caccttggcg cagcgggtgc
     5041 atttgaagtg cccgtgctga tggtgcggca gccggggcga ggaatcgcct gcggcatgcg
     5101 gcgcgccggc gctgtagcgg aacacgcggt cgtcgccgga aatcttgtgg gcgagaccgg
     5161 cttcggtcag gcagtcgagc gtgcggtaca gcgttacccg gtccatgtcg gcgatcgctt
     5221 cctggacgtc atggtgcgac agcgcccgcg gcgaggcgag cagcgcgcag agcacgcggg
     5281 tgcgggcgtc ggtgacccgc accgccgcgt cgcgcagcag cgcttgcgcg cgcgccgcgt
     5341 gggtctttgc ctcgttgtcg gacggcgccg gctcgcgcgg caggggatgt ctcgtactca
     5401 tgcgtgtgct gctgtcggag ggatgctccg attatcgctc caaaatgcaa ttc
//
LOCUS       DATLGF010000235         2696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_226921, whole genome shotgun sequence.
ACCESSION   DATLGF010000235 DATLGF010000000
VERSION     DATLGF010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226921"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1433)
                     /locus_tag="VM406_07080"
     CDS             complement(<1..1433)
                     /locus_tag="VM406_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701936.1"
                     /note="catalyze the formation of cyanophycin which may act
                     to store excess nitrogen; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HVL75762.1"
                     /translation="MQVTRIRALRGPNLWSAHTAIEAVVHCEPFECDARAMPGFEARL
                     RERFPGMEPLQPRGHDGSVSLAHVLELAALDLQAHAGCPVTFSRIAPTDETGVYRVVV
                     AYSEEAVGRRALELAGQLCRAAVDGSGFDLEAALAELRELDEDVRLGPSTGAIVNAAL
                     ARDIPYRRLTAGSMVMFGWGSRARRIQAAETDTTSAIAESIAQDKELTKKLLASAGVP
                     VPLGRIVSGAEDAWTAACAIGLPVVVKPRDGNQGKGVTVNIASREHMRAAFEAAARHG
                     DEVIVERYLPGNDFRLLVVGNKLVAAARRDPPHVVGDGMHTVRELVDIVNSDPRRGTG
                     HATSLTRIRIDEIAQARLQLQGLNADSVPALGQRVVLRNNANLSTGGTATDVTDDVHP
                     DVAASAVAAAQMIGLDICGVDLVCDSVHRPIEEQGGGIVEVNAAPGLRMHLAPSYGKG
                     RMVGEAIINEMFPEGDDGRIPVVAVTGT"
     gene            complement(1677..>2696)
                     /locus_tag="VM406_07085"
     CDS             complement(1677..>2696)
                     /locus_tag="VM406_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HVL75763.1"
                     /translation="VLIQRNGNVAFDVTDAVHPDVAAMATLAARVVGLDIAGIDLVAE
                     DISRPLQEQRGAIIEVNASPGLLAHIKPASGTPRAVGAAIVDHLFATGENGRIPVVGI
                     AGSRGTTRIAQLVAWIYYVSGRHTGLASRNGLTVNGRNVPAPEATPWEAAQRLLVNRA
                     VEAAVFENDSRMILSEGLPYDRCAVGVVTDAFVQPELAEFYIDRPEQVFNVLRTQVDV
                     VLPDGVAVLNAADARVAEMASLCDGRVLFYGLARTLAPLAAHRHAGGRAVFLHRDSIV
                     LADGAREAARVPMPAGTADPEPVVAAVAAAWAAGIDPQLISAGLRTFAPLYPRMPSEQ
                     GVVSA"
BASE COUNT          404 a          939 c          922 g          431 t
ORIGIN      
        1 gtgccggtga ccgcaacgac cgggatgcgg ccgtcatcgc cttcgggaaa catttcgttg
       61 atgatcgcct cccccaccat gcggcccttg ccgtaggacg gcgcgaggtg catgcgcaag
      121 ccgggcgcgg cattcacctc gacgatgccg ccgccctgct cttcgatcgg cctgtgcacg
      181 ctgtcgcaga caaggtcgac gccgcagatg tccagcccga tcatctgcgc cgccgccacg
      241 gcgctcgcgg ccacgtccgg atgcacgtcg tcggtgacgt cggtggcggt gccgccggtg
      301 gacaggttgg cattgttgcg caggaccacg cgctgcccga gcgcgggcac gctgtcggcg
      361 ttcaggcctt gcagttgcag gcgcgcctgt gcgatctcgt cgatgcggat tcttgtgagc
      421 gaggtggcgt gacccgtgcc gcggcgcgga tcgctgttga cgatgtcgac cagctcgcgc
      481 actgtgtgca tgccgtcgcc gaccacatgc ggcggatcgc gccgtgccgc ggcgaccagc
      541 ttgttgccga ccaccagcag gcggaaatcg ttgccgggca ggtagcgttc gacgatgacc
      601 tcgtcgccat gccgggcagc ggcctcgaac gccgcgcgca tgtgctcgcg cgaggcgatg
      661 ttgaccgtca cgcccttgcc ctggttgccg tcgcgcggct tgaccacgac cggcaggccg
      721 attgcgcagg cagccgtcca ggcgtcttcc gcgccggaaa cgatccggcc cagcggcact
      781 ggcacaccgg ccgaggcaag cagcttcttc gtcagttcct tgtcctgtgc gatcgattcg
      841 gcaatcgcgc tggtcgtatc ggtttcggcg gcctggatgc ggcgcgcgcg gctgccccag
      901 ccaaacatga ccatgctgcc ggcagtgagg cgacggtacg gaatatcgcg ggcgagcgcc
      961 gcgttgacga tggcaccggt gctggggccc aggcgcacgt cctcgtcgag ttcgcgcagt
     1021 tcggccagtg ccgcctccag gtcgaacccg ctgccgtcga cggcggcgcg gcacagctgc
     1081 ccggccagtt ccagcgcgcg gcggccgacc gcctcttccg aatacgcgac cacgacacga
     1141 tagacgccgg tctcgtccgt cggagcgatg cggctgaagg tcaccgggca gcccgcatgc
     1201 gcctgcagat ccagcgcggc cagttccagc acatgcgcca gcgagacgct gccgtcatgc
     1261 ccgcgcggct gcaagggttc catgccggga aagcgctcgc gcaaccgcgc ctcgaagccc
     1321 ggcatcgcgc gcgcgtcgca ctcgaacggc tcgcagtgga cgacagcctc tatcgcggtg
     1381 tgcgcgctcc agaggttcgg tccgcgcagc gcgcggatgc gggtgacttg catggaagtg
     1441 ttcctggata aaaggggaat tgtctggacg ttggggaatg cttgtcacca cggcgagtac
     1501 gacgaacaag ctcaccctgc tgtcgtcccg ggcgcagcga gggacctcat cggcttatcc
     1561 actggagatt cctcgttcgg ctcggaatga caggtttttt gcgtgctcgc catggttcgc
     1621 gttttcgaag atgtctttcc cggcggcgcg cagccggtac ccgccgccgg ccgcgctcac
     1681 gcgctcacga caccctgttc ggaaggcatg cgcggataca gcggcgcgaa cgtgcgcagt
     1741 ccggcgctga tcagctgggg gtcgatgcct gccgcccagg cggcggctac cgccgccacc
     1801 actggctcgg ggtcggccgt acccgcgggc atcggcaccc gcgccgcttc acgcgcgccg
     1861 tctgccagca cgatgctgtc gcggtgcagg aacaccgcgc gcccgcccgc gtggcgatgc
     1921 gcggccagcg gcgcgagcgt gcgcgcgagg ccgtagaaca gcacgcgccc gtcgcacagg
     1981 gaagccattt cggccacccg tgcatccgcc gcgttcagga ccgccacgcc atcgggcagc
     2041 accacgtcga cctgggtgcg cagcacgttg aacacctgtt cgggccggtc gatgtagaac
     2101 tcggccagct ccggctgcac gaacgcatcg gtgaccacgc cgaccgcgca cctgtcatag
     2161 ggaaggccct cggaaagaat catgcggctg tcgttctcga acaccgccgc ctccaccgcc
     2221 cggttcacca gcagccgctg cgccgcttcc caaggcgtgg cttccggcgc cggtacattg
     2281 cgaccgttga cggtcagtcc gtttcggctg gccaggccgg tgtggcgccc gctcacgtaa
     2341 taaatccagg cgaccagctg ggcaatgcgc gtggtgccac gcgagccggc aatgccaacc
     2401 accgggatgc ggccgttctc gccggtggcg aacaggtgat cgacgatggc ggcgcccact
     2461 gcgcgcggcg tcccggaggc cggcttgata tgcgcaagca ggcccggact ggcattgact
     2521 tcgatgatcg cgccgcgctg ctcctgcagc ggacgcgaga tgtcttcggc gaccaggtcg
     2581 atgccggcaa tgtcgaggcc gaccacgcgc gcggccagcg tggccatcgc ggccacatcg
     2641 ggatgcaccg cgtcggtgac gtcgaaggcg acgttgccgt tgcgctggat gagcac
//
LOCUS       DATLGF010000236         4560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_57014,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000236 DATLGF010000000
VERSION     DATLGF010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57014"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /gene="nrdR"
                     /locus_tag="VM406_07090"
     CDS             complement(<1..360)
                     /gene="nrdR"
                     /locus_tag="VM406_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HVL75764.1"
                     /translation="MKCPFCDHEDTQVIDSRTSEEGDSVRRRRKCTHCDKRFTTYERV
                     ELSMPVIVKKDGSRTDYEPAKLRASMMLALRKRPVSAEAVDEAIRSIEDKLRSSGARE
                     VVSGHLGELVMRELKRLD"
     gene            complement(420..1664)
                     /gene="glyA"
                     /locus_tag="VM406_07095"
     CDS             complement(420..1664)
                     /gene="glyA"
                     /locus_tag="VM406_07095"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HVL75765.1"
                     /translation="MFAKDHTIANVDPELWAAIQQENQRQQDHIELIASENYTSPAVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEFVDIAETLAINRLKELFGAEAANVQPNSGSQAN
                     QGVFFAMLKPGDTIMGMSLAEGGHLTHGMALNMSGKWFNVVSYGLNEKEEIDYEAMER
                     LAHEKKPKLIIAGASAYALKIDFERMAKVARDVGAYFMVDMAHYAGLIAAGVYPNPVP
                     YADFVTSTTHKSLRGPRGGVILMKAEHEKAINSAIFPGIQGGPLMHVIAAKAVAFKEA
                     LSPEFKAYQQQVVKNADALAKALIARGLRIVSGRTESHVMLVDLRAKKITGKEAEAIL
                     GSAHITCNKNAIPNDPEKPFVTSGIRLGSPAMTTRGFKEAEATKVGNLIADVLDNPHD
                     AATIERVKAEVKKLTDAFPVYA"
     regulatory      complement(1775..1887)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            1999..2751
                     /locus_tag="VM406_07100"
     CDS             1999..2751
                     /locus_tag="VM406_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL75766.1"
                     /translation="MIVLITGASSGFGAEMARRFAQSGHRVIAAARRADRLDQLAAEL
                     GANLLPLQMDVSDKASISGALASLPPEWKTIDVLINNAGLALGIEPAQRASLEEWETM
                     INTNCKGLVTITHALLPEMVARGSGMIINIGSTAGAYPYPGGNVYGATKAFVDQFTLN
                     LRADLVGTGVRATNIAPGLCGGTEFSNVRLKDDAAAAKVYEGTVPLTAKDIAETAFWI
                     ATLPAHVNINYIEMMPTCQGFSPFNIKRNLAA"
     gene            2757..3185
                     /gene="ybgC"
                     /locus_tag="VM406_07105"
     CDS             2757..3185
                     /gene="ybgC"
                     /locus_tag="VM406_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HVL75767.1"
                     /translation="MAGSEAPFCWKVRVYYEDTDTGGVVFYANYLKFFERARTEWLRA
                     AGIGQQQLAETHGAIFVVKHTEVDYHAPAKLDDELKLSVVVERLGRASVQFIQEAWRI
                     QGAQADLLATGRIRVGCVDTATFRPRPIPDTVLARIKTSG"
     gene            3258..3959
                     /gene="tolQ"
                     /locus_tag="VM406_07110"
     CDS             3258..3959
                     /gene="tolQ"
                     /locus_tag="VM406_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HVL75768.1"
                     /translation="MTVTQDLSLIALISEASLLVQLVMALLVLLSLMSWTYIFTKMFA
                     IRSAARQTEAFERSFWSGGNLNALYEEASTNRRRTDNRGAALERIFQAGMSEFQKVKL
                     SLGARGQADSGVMIDGARRAMRAAYQREMDALESHLAFLASVGSVSPYIGLFGTVWGI
                     MNAFRGLANVQQATLASVAPGIAEALVATAIGLFAAIPAVVAYNRYSHDLDRLANRFE
                     SFIEEFSNILQRQVR"
     gene            3959..4387
                     /locus_tag="VM406_07115"
     CDS             3959..4387
                     /locus_tag="VM406_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HVL75769.1"
                     /translation="MASMRGGRGRRFKSEINVVPYIDVMLVLLVIFMVAAPMATPGVI
                     NLPTAAKTSLPPTEYIHITMKSDANATIALGVQGSRPVQEETVARAALVTRLAALHKS
                     NPEMPVMISAEKDIRYEEVIRVISDAKRIGINRVGLATRE"
BASE COUNT          793 a         1444 c         1462 g          861 t
ORIGIN      
        1 gtccaggcgc ttgagttcgc gcatcacgag ttcgccgagg tggccggaca cgacttcccg
       61 cgcgcccgat gagcgcagct tgtcttcgat gctgcgtatc gcctcgtcta ccgcctcggc
      121 ggaaaccgga cgcttgcgca gcgccagcat catgctggcg cgcagcttgg ccggttcgta
      181 gtcggtccgg ctgccgtcct tcttgacgat caccggcatc gacagctcga cccgttcata
      241 ggtcgtgaag cgcttgtcgc aatgcgtgca tttgcggcgg cggcggaccg aatcgccctc
      301 ttccgaggta cggctgtcga tgacctgggt gtcttcgtgg tcgcagaacg ggcacttcat
      361 gtcggcaccg ccgggtggga gtgaaatggg cggcggctgg aatgccgccg ccggcacgct
      421 tacgcgtaga ccgggaacgc gtcggtcagc ttcttgactt ccgccttcac acgctcaatc
      481 gtagcggcat cgtgcggatt gtccagcacg tcggcaatca ggttgccgac cttggtcgcc
      541 tcggcttcct tgaagccgcg cgtggtcatc gccgggctgc cgagccggat gcccgaggtg
      601 acgaaaggct tttccgggtc gttcggaatc gcattcttgt tgcaggtgat gtgggccgaa
      661 ccaaggatgg cttcggcttc cttgccggtg atcttcttcg cacgcaggtc gaccagcatc
      721 acgtgcgatt cggtgcgccc ggacacgatg cgcaggccgc gtgcgatcag cgccttggcc
      781 agcgcgtcgg cgttcttcac cacctgctgc tggtaggcct tgaattccgg cgacagcgcc
      841 tccttgaagg cgaccgcctt ggcggcgatc acatgcatca gcggaccgcc ctggatgccg
      901 gggaaaatcg cggagttgat cgccttttcg tgctcggcct tcatcaggat cacgccgccg
      961 cgcgggccgc gcagcgactt gtgggtggtc gaggtgacga agtccgcgta cggaaccgga
     1021 ttcgggtaga cgccggcggc gatcaggccc gcatagtggg ccatgtccac catgaaatac
     1081 gcgccgacgt ccctggcgac cttcgccatg cgctcgaagt cgatcttcag cgcataggcg
     1141 gaggcgccgg cgatgatcag cttgggcttt ttctcgtgcg ccaggcgctc catcgcctcg
     1201 tagtcgatct cttccttctc gttcagtccg taggacacga cgttgaacca cttgccggac
     1261 atgttcagcg ccatgccatg cgtcagatgg ccgccttcgg ccagcgacat gcccatgatg
     1321 gtgtcgcccg gcttgagcat cgcgaagaac acgccctggt tcgcttgcga gccggaattc
     1381 ggctgcacgt tcgccgcctc ggcaccgaac agttccttca gacggttgat ggccagcgtc
     1441 tcagcgatgt cgacgaattc gcagccgccg taatagcgct tgcccggata gccttcggcg
     1501 tacttgttgg tcagctgcga accctgcgcc tccatcacgg ccggcgaggt gtaattctcg
     1561 gacgcgatca gctcgatgtg gtcctgctgg cgctggttct cctgctggat ggccgcccag
     1621 agctccgggt cgacgttggc gatggtgtgg tctttggcga acatgtataa ctcctggcaa
     1681 tgcgaaactg ggttgggcgc gagcctgtga tgcggcgctg cgagcgagag ctggagaaaa
     1741 tagacaaggg gcagcctcga tcgtgcattt ccatttgggc tgcccaggcg aacggcgtag
     1801 atactgcgga agcctcacgt tccccggtgg ttgcccacct tgaaccgggc cggtggcctg
     1861 gtcgtttcgc cagtcacgtg aggtgaaatg gtcccgaatt gtaatttacg gccttgaatt
     1921 cggcaacaag cgggcgctgc cggatacggg agcgcgggtt acaatttcgc ccgtttaccg
     1981 tcagctacag gaagtcacat gatcgtcttg attaccggag ccagttccgg ctttggcgcc
     2041 gagatggcgc gccgcttcgc ccagtcgggc cacagggtca tcgccgccgc gcgcagagcc
     2101 gaccgcctcg accagctcgc cgccgagctc ggcgcgaacc tgctgccgct ccagatggat
     2161 gtcagcgaca aggcgtcgat cagcggcgcg ctggccagcc tgccgccgga gtggaagacg
     2221 atcgacgtgt tgatcaataa cgccggcctc gcgctcggca tcgagccggc ccagcgcgct
     2281 tcgctggaag agtgggagac gatgatcaac accaattgca agggtttggt gacgatcacg
     2341 cacgcgctgc ttccggagat ggtcgcgcgc ggcagcggca tgatcattaa catcggctcg
     2401 accgccggcg cctatcccta cccgggcggc aacgtctatg gcgcgaccaa ggccttcgtc
     2461 gaccagttca cgctgaacct gcgcgcggat ctcgtcggca ccggcgtacg cgcaaccaac
     2521 atcgcacccg gcctgtgcgg cggcaccgaa ttctcgaatg tgcgcctgaa ggatgacgcg
     2581 gccgcggcca aggtctacga gggcacggtg ccgctgacgg cgaaggacat tgccgagacg
     2641 gcgttctgga tcgccaccct gcccgcccac gtcaacatca attacatcga gatgatgccg
     2701 acctgccagg gcttctcgcc gttcaacatc aagcgcaacc tggccgcgta atgacgatgg
     2761 ctggatcgga ggcgcccttc tgctggaagg tgcgggtgta ctacgaggac acagacaccg
     2821 gcggcgtcgt tttttacgcg aactatctca agtttttcga gcgggctcgc acggaatggc
     2881 tgcgggcggc cggcatcgga cagcagcaac tggccgaaac ccacggggcg atcttcgtcg
     2941 tcaagcacac cgaagtcgat tatcatgccc cggcgaaact agatgatgaa ctgaaactct
     3001 cggtcgttgt cgaacgcctc ggccgtgctt ccgtgcaatt tatccaggag gcatggcgga
     3061 tacagggagc gcaagcggat cttctcgcaa ccggtcgcat acgcgtcggc tgcgtggata
     3121 ccgcgacgtt caggccgcgg ccgataccgg acacggtgct ggcccgcata aaaacatcag
     3181 gataaccgcg tcgacgcggg gtgccggccc gaaggccgcg ccttgcgcgg cacgctgtcc
     3241 gaaaccagtc gaatcagatg accgtcaccc aagatctttc cctcatcgca ctgatcagcg
     3301 aagcctcgct gctcgtccag ctggtcatgg cgcttctggt cctgctctcg ctgatgagct
     3361 ggacttacat ctttaccaag atgtttgcga tccgcagcgc cgcgcgccag accgaggctt
     3421 tcgagcgcag cttctggtcc gggggaaatt tgaatgccct gtacgaggaa gccagcacga
     3481 atcgccgccg caccgacaac cgcggcgcgg cgctcgagcg catcttccag gccggcatga
     3541 gtgaattcca gaaggtcaag ctgagcctcg gcgcccgcgg gcaggccgat tccggcgtga
     3601 tgatcgacgg cgcccggcgt gcgatgcgtg cggcttacca acgcgagatg gacgcactcg
     3661 aatcgcatct cgcgtttctc gcctcggtcg ggtcggtgtc gccgtatatc ggcctgttcg
     3721 gcaccgtgtg ggggatcatg aacgcgttcc gcggactggc caatgtccag caggcgacgc
     3781 tggcctcggt cgccccgggc atcgcggaag cgctggtggc gacggcgatc ggcctgttcg
     3841 cggcgattcc ggcggtggtg gcctacaacc gctattcgca tgacctcgac cgcctcgcca
     3901 accgtttcga gagcttcatc gaggagttct cgaacatcct gcagcgacag gtgcgctgat
     3961 ggcatcgatg cgcggcggcc gcggccgcag gttcaagtcc gagatcaacg tcgtgcctta
     4021 catcgacgtg atgctggtgc tgctcgtcat tttcatggtg gccgccccga tggcgacccc
     4081 aggcgtgatc aacctgccga cggcggccaa gacctcgttg ccgcccaccg aatacattca
     4141 catcacgatg aagtccgatg cgaacgcgac gatcgcgctc ggcgtccagg gcagccgccc
     4201 ggtccaggaa gagacggtgg cgcgcgccgc tctcgtcacc cggcttgccg cgctgcacaa
     4261 gagcaatccg gagatgccgg tgatgatctc ggccgaaaag gacatccggt atgaggaagt
     4321 gatccgggtc atcagcgacg ccaaacgcat cggcatcaac cgggtaggac tggcgaccag
     4381 ggaatgacgg acaccacgcc ttacaccgta ccgaaggaaa ggggacgctg gcgcgcgcta
     4441 ctgctcgcgc tggtcgtgca tgtcgcgctt ttcctgttgc tctggatcgg cgtgtcgtgg
     4501 cagagcgaga caccgggcac gatcgaggcc gaggtctgga gccttcagcc ccagtccgcg
//
LOCUS       DATLGF010000237        11396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_136858, whole genome shotgun sequence.
ACCESSION   DATLGF010000237 DATLGF010000000
VERSION     DATLGF010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 11396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11396
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136858"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VM406_07120"
     CDS             <1..342
                     /locus_tag="VM406_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HVL75770.1"
                     /translation="RLDRALERERSAAERRAEAQARRAREEAEASREKAERASAPAAP
                     DEAKPVPKPEPAAVRLPKPAPTPEQEQEEALRRARNEEVYARKVEAAQARREATERKK
                     AEKEQRRAQRD"
     gene            complement(378..1184)
                     /locus_tag="VM406_07125"
     CDS             complement(378..1184)
                     /locus_tag="VM406_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HVL75771.1"
                     /translation="MGHYKIEAGTGQHLGDRGEQQDRVALYTAPKAPGYVMAILADGM
                     GGMSGGSMAAEQVLRTARQAFDLFSPLTDDVAKMLEAIARDAHTIIKLTSFAAEKNPH
                     STMVALVLTPQREAFWAHVGDSRLYRFDGPNFASRTRDHSYVEDLVSQGKLAREKAAG
                     HPMANVLLSALGSHDNEPEVSLGRHTGLKAGDAFLLCSDGLWHYLNESELGAAIAMNP
                     PRQASEMLIQKARERAAGEKADNCSLAIVRLVPAEDTAQKTKVGKMRRAV"
     gene            1367..1567
                     /locus_tag="VM406_07130"
     CDS             1367..1567
                     /locus_tag="VM406_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HVL75772.1"
                     /translation="MTSKEDIRRRIVELEVEHRDLDSVIDLLANEVLRDELQLRRLKK
                     RRLQLKDHITLLRMQLVPDIPA"
     gene            1729..3603
                     /locus_tag="VM406_07135"
     CDS             1729..3603
                     /locus_tag="VM406_07135"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HVL75773.1"
                     /translation="MAEAIAAAIAERRTLIAEAGTGTGKTFAYLVPALLWGGKVILST
                     GTKNLQDQLYLRDIPTVRRALNAPVSVALLKGRANYVCHYHFERTQQNGRLTSREDVG
                     YLREIGRFIKTTSSGDKAELARVPESASVWNLVTSTRENCLGADCQYYQDCFVMKARK
                     EAQQADVVVVNHHLFFADVALKDTGVAELLPSANTVIFDEAHQLPETATLFFGESVST
                     SQVLELCRDVLAEGRAHARDGADWGQVVVPVEKAARDLRLAFPQDVVRLSVAQIAPSS
                     AFFPALDALQAQLGAMLEVLERQAERAETLEQCRVRALELARRLDAWRAARDVEPTGE
                     AQERVLWVEAFSSSLQLHQTPLSIAPIFSRQREGTPRAWIFTSATLAVKQDFTHYASQ
                     MGLWNEAAQSWPSPFDYQAQGLLYVPHNLPDPNSHDYTDAVVDAALPLIEAAGGRAFL
                     LCTTLRAVTRAAERLRAEFERRGWPFPLMVQGEAGRTELLDRFRSAGNAVLVGSQSFW
                     EGVDVRGDALSLVVIDKLPFSPPDDPVLAARIEAMERKGLNGFVHHQLPAAIINLKQG
                     AGRLIRDETNRGVLMICDPRLISKPYGRRIWQSLPPFRRTRERDVACAFLQQVDAPAA
                     "
     gene            complement(3623..4603)
                     /locus_tag="VM406_07140"
     CDS             complement(3623..4603)
                     /locus_tag="VM406_07140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HVL75774.1"
                     /translation="MKAVVCKAWGPPDSLVVEELPDVVPGPGQVAIDVQAAGVNFPDV
                     LIIQNKYQAKPQLPFTPGSELAGVVRAVGEGVTHLKPGDKVLAFVSQGAFAQQIAVPA
                     AAAMPMPPGLDFDTAAAVTLTYGTSHHAVVDRAQLKAGETMLVLGAAGGVGLAAIEIG
                     KALGARVIAAASSDDKLAVCREHGADLTINYSTTDLREAIKAATDGKGPNVIYDPVGG
                     EYTEPAFRSIAWRGRYLVIGFANGEIPKLPLNLPLLKGASVVGVFWGEFAKREPKANL
                     AAMRELMEWMGQGKIRPHISARYGLAETARALNDMAARKVTGKVVIQPYK"
     gene            4730..5479
                     /gene="surE"
                     /locus_tag="VM406_07145"
     CDS             4730..5479
                     /gene="surE"
                     /locus_tag="VM406_07145"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HVL75775.1"
                     /translation="MKILVSNDDGYLAPGLIALAEALKPIADIVVVAPDSNRSGSSNS
                     LTLDRPLSVYRAENGFYFVNGTPSDCVHIALTGVLDEKPDLVVSGINQGQNMGDDTLY
                     SGTVAAATEGFLFGIPAIAFSQVEKGWAHLESAARIAREIVERSADRGLGALGRPYLL
                     NVNIPNLPYEQIPGLRPSRLGKRHESEAVIRSTDPHGREIYWIGPAGRAKDAGEGTDF
                     HAVTNGYVSITPLQVDLTHTEQLRALQQGLV"
     gene            5476..6294
                     /locus_tag="VM406_07150"
     CDS             5476..6294
                     /locus_tag="VM406_07150"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HVL75776.1"
                     /translation="MNAKEKRFPLSLDSVVSKAREAAGSRSASSGTAQTASRNALAHQ
                     TRAPVPSMEAPRVAPVVSDAVRGAMVGRVAQQGVKDKKVLAALEAIPRHLFVQPALAS
                     QAYIDASLPIGHHQTISQPYIVARMIEVLRDNKAGGRLERVLEIGTGCGYQAAVLSLV
                     AVEVYSVERIKPLHELAKSNLRPLRIPNIRLQYGDGMLGLPQAAPFDGIILAAAGLEV
                     PQALLEQLSIGGRLVAPVGEQRQVLQLIERVAQREWKTTTLEGCHFVPLRPGVI"
     gene            6348..7289
                     /locus_tag="VM406_07155"
     CDS             6348..7289
                     /locus_tag="VM406_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HVL75777.1"
                     /translation="MRKSSFTVVAFIPVLLAACSTATRAPAPVLDRTSAARPVENGRP
                     GVAANQVPKQADARGFYTVKRGDTLIQIALEFGQNYRDLVVWNNLANPNDIKVDQVLR
                     VLPPETGAQTASVATTPGVEVRPLAPGAAAGTSKTSPRGDKRPYSDAALAEMQKGEGE
                     PTPSAQASAPAASVPGSPARGPQNAAAPLPDDENISWIWPAEGRVISQFGEGRRGIAI
                     AGQMGQPVLAAGSGKVLYAGSGIRGYGNLVIVKHTNNLLSAYAHNKTIFVKEDQTVAQ
                     GQKIAEMGNSDTDEVKLHFEIRHQGKPVDPSKFLPNR"
     gene            7308..8327
                     /gene="rpoS"
                     /locus_tag="VM406_07160"
     CDS             7308..8327
                     /gene="rpoS"
                     /locus_tag="VM406_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HVL75778.1"
                     /translation="MNRKAPDSMDDDEPTDDLSDAMAHGAEDGLAPDGEGAVDAGEIG
                     HAPAEPVDELKTVLAAELATDTTQHYLNQIGARPLLTVAEEVHYATLAKQGNFEARQK
                     MIEHNLRLVVSIAKHYINRGVVLLDLIEEGNLGLMRAIDKFEPERGFRFSTYATWWIR
                     QSIERAIMNQARTVRLPVHMVRELNQILRAKYHLEAQHHDGKEASAEDIAHLVDRQVE
                     DVQDILALSEHATSLDAPLDNDPQASLMDMLPGDAEDGPDHQAEHHEMTVLVRDWLKK
                     LPDKQRIVIVRRFGLDNDDPATLEELAAEMSVTRERVRQIQQEALVKLKRALTARGVG
                     KDALL"
     gene            8324..9106
                     /locus_tag="VM406_07165"
     CDS             8324..9106
                     /locus_tag="VM406_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HVL75779.1"
                     /translation="MMPVLVFDIETIPDVAGLRALNAFPASMSDAEVAAAAFAARREK
                     TGSDFLQHHLHRIAAISCVFRDNDGFRVRSLGGLEDGEQKLVADFFRVIDRYTPQLVS
                     WNGGGFDLPVLHYRALLHGVSAARYWEMGDEDRDFKWNNYLSRYHTRHMDLMDLLALY
                     TGRANAPLDDLAKLCGFPGKLGVDGSQVWNLYQEGRLGEIRAYCETDVVNTYLVYCRF
                     QLMRGSLSMEAYREELSLVRHSLGGLEGEHWKEYLSAWPSQA"
     gene            9168..10508
                     /gene="rlmD"
                     /locus_tag="VM406_07170"
     CDS             9168..10508
                     /gene="rlmD"
                     /locus_tag="VM406_07170"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HVL75780.1"
                     /translation="MQQNVILIESLDMEGRGVGHQVNEDGSPGKVIFVEGALPGERVE
                     YQSFRRKPKWEAATLTALHNESALRVQPKCVHFGTCGGCAMQHLEPGAQVAVKQRVLE
                     DNLWHLGKVRAETMLAPVHGPTWGYRYRARLSVRHVAKKGGMLVGFHERKSSFIADMT
                     SCQILPPHVSDMLVPLRGLVGSLSIFDRMPQIELAVGEGVTALVLRNMAPLTTDDEDR
                     LRAFADTHGVQWWLQPKGPDTVYPFYPEQSTLCYTLPEFGVRMPFKPTDFTQVNHQIN
                     RVLVSKALRLLDMQPNERVADLFCGLGNFTLPLATRAAEVVGIEGSTALTERALENAR
                     LNGLDTKTSFHCRNLFEATAADFAALGRFDRMLIDPPREGALAVCQALVELGKADASM
                     RPRRIVYVSCNPSTLARDAGYLVHQGGYTLRQAGVVNMFPHTAHVESIAVFDAA"
     gene            10603..11028
                     /gene="ndk"
                     /locus_tag="VM406_07175"
     CDS             10603..11028
                     /gene="ndk"
                     /locus_tag="VM406_07175"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HVL75781.1"
                     /translation="MAIERTLSIIKPDAVAKNVIGQIYTRFENAGLKIVAARMAQLSR
                     AEAEGFYAVHRERPFFKDLVDFMVSGPVMIQVLEGENAIAKNRELMGATDPKKADKGT
                     IRADFADSIDANAVHGSDAPDTAKVEIAYFFPALNVYSR"
     gene            11069..>11396
                     /locus_tag="VM406_07180"
     CDS             11069..>11396
                     /locus_tag="VM406_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HVL75782.1"
                     /translation="MTALTNLLDLDPAQLVAYCAELGEKPFRAKQLQRWIHQFGASDF
                     DEMTDLAKSLREKLKTRATIAAPAIISDHTSSDGTRKWLVDVGQGNAVETVFIPEENR
                     GTLCIST"
BASE COUNT         2079 a         3648 c         3645 g         2024 t
ORIGIN      
        1 cgccttgacc gcgcgctcga acgcgaacgc agtgccgccg aacggcgggc cgaggcgcag
       61 gcccggcgcg cgcgcgaaga ggcggaagcg agtcgcgaga aagccgagcg cgcttcggcg
      121 ccggcggcgc cggatgaggc aaagccggtc ccgaagcctg aaccggcagc ggttcgcctg
      181 ccgaagccgg ccccgacccc ggagcaggaa caggaagagg cgctgcgccg cgcccgcaat
      241 gaagaggttt atgcgcgcaa ggtggaagcg gcccaggcgc ggcgcgaggc aaccgagcgc
      301 aagaaagccg agaaggagca gcggcgcgcg cagcgtgact gagcactgcg cacggtcctg
      361 ttcaaaccct cagatcatca aaccgcacgc cgcatctttc cgaccttcgt cttctgggcc
      421 gtgtcttcgg ccggcacgag cctcacgatg gcaaggctgc aattgtcggc cttttctccg
      481 gccgcgcgtt cacgtgcctt ctggatcagc atttccgatg cctggcgcgg cgggttcatc
      541 gcaatcgccg cgccgagttc agactcgttc aggtagtgcc acagaccgtc gctgcacagg
      601 aggaaggcat cgccggcctt caggccggtg tggcggccaa gcgacacctc gggttcgttg
      661 tcgtgcgaac cgagcgcgct gagcagcacg ttggccatcg gatgaccggc cgccttctcg
      721 cgtgcgagct tgccttggct gacaaggtct tccacatagg agtgatcgcg ggtgcgcgag
      781 gcgaagttgg ggccgtcgaa acggtacagc cgcgaatcgc ccacgtgcgc ccagaatgcc
      841 tcgcgctgcg gcgtgagaac cagggcgacc atcgtgctgt gcgggttctt ctcggcggcg
      901 aaggatgtga gcttgatgat ggtgtgggca tcacgcgcga ttgcctcgag catcttcgcc
      961 acgtcgtcgg tgagggggga gaacaggtcg aatgcctgcc gggcggtgcg cagcacctgt
     1021 tccgcggcca tcgagccgcc gctcatgccg cccatgccgt cggccagaat cgccatcaca
     1081 tagccggggg ctttcggtgc cgtgtagagc gccacgcggt cctgctgctc gccgcggtcg
     1141 ccgagatgct ggccggtgcc cgcttcgatc ttgtagtggc ccatagaagg actggtaagg
     1201 actggtcaga aaaacgggga attattgcat tcggaaagac tggcggcgat ttctggcaag
     1261 caattgttac acgccgcgca tcggtgccgg acaggcgctc gccagcgcct agccgctgga
     1321 gtgattcaat cctacaatac cggttatcgc actcccatcg tggatcatga catcaaaaga
     1381 agacatccgc cgcaggatcg ttgaactcga agtcgagcac cgggatctgg attccgtgat
     1441 cgacctgctc gccaacgagg ttttgcgcga cgaactgcag ttgcggcgtc tgaagaagcg
     1501 cagactgcag ctgaaggatc atatcaccct gctgcgaatg caactggtac ccgacattcc
     1561 cgcctgacaa cccggcttgc accgggcaat ttccctgcct tgaccgacgc tccctccgtt
     1621 ctgccacccg ctcccgacca cgagggcgag gtcgcccgcc tgtttgcggt cggcggcccg
     1681 ctttccgaag cggttgccgg ctttcgtccg cgccagtccc agaccgagat ggccgaggcg
     1741 atcgccgcag ccattgccga acgcaggacg ctgatcgccg aagcgggcac cggcaccggc
     1801 aaaactttcg cctatctcgt gcccgcgctg ctgtggggcg ggaaggtgat cctgtccact
     1861 gggaccaaga acctgcagga ccagctctat ctgcgtgaca ttccgaccgt gcgccgggcc
     1921 ctgaacgccc cggtgtcggt tgcgttgctg aaggggcggg ccaactacgt ctgtcattac
     1981 catttcgaac gcacgcaaca gaacggccgg ctcacgtccc gcgaagacgt gggttatctg
     2041 cgcgagattg gacgcttcat caagaccacg tcctccggcg acaaggccga gctggcgcgc
     2101 gtgcccgagt cggcgtcggt gtggaacctg gtcacgtcca cgcgcgagaa ctgcctcggc
     2161 gccgattgcc agtattacca ggactgtttc gtgatgaagg cgcgcaagga agcgcagcag
     2221 gccgacgtgg tggtcgtgaa tcaccacctg ttcttcgccg acgtcgcgct caaggacacc
     2281 ggggtggccg aactgctgcc gtcggcgaat accgtgatct tcgacgaggc gcaccagctg
     2341 cccgagaccg cgacgctgtt cttcggcgaa tcggtttcga cttcgcaggt gctcgaactg
     2401 tgccgcgacg tgctggccga aggacgcgcc cacgcgcgcg acggggcgga ctggggacag
     2461 gtggtggtgc cggtggaaaa agccgcgcgc gacttgcgcc tggcgtttcc ccaggacgtc
     2521 gtccggctgt cggtggcgca gatcgcgccg tcgagcgcct tcttccccgc actggacgcg
     2581 ctgcaggcgc agctgggtgc gatgctcgag gttctcgagc gccaggcgga gcgcgccgag
     2641 accctcgagc aatgccgcgt gcgcgcgctc gaactggcac gccggctgga tgcatggaga
     2701 gccgcccgcg acgtggaacc gaccggcgaa gcccaggagc gcgtactgtg ggtggaagca
     2761 ttttcctcct cgctgcagct gcatcagacg ccgttgtcga tcgcgcccat cttcagcagg
     2821 cagcgcgagg gtacgccgcg cgcctggatc tttacgtcgg cgacgttggc ggtcaagcag
     2881 gatttcaccc actatgcgtc gcagatgggc ttgtggaacg aggctgcgca atcctggccc
     2941 agccccttcg actatcaggc gcagggcttg ctttacgtcc cgcacaacct acctgatccg
     3001 aattcgcacg attacaccga cgccgttgtc gacgccgcct tgcccctgat cgaggcggcg
     3061 ggcggacgcg ccttcctgct ttgcacgacg ctgcgcgcgg tcacgcgggc cgctgagcgg
     3121 ctgcgcgccg agttcgagcg tcgcggctgg ccgttcccgc tgatggtcca gggcgaggcg
     3181 ggccgcaccg aactgctcga tcgcttcagg agcgcgggca atgcggtact cgtgggcagc
     3241 cagagtttct gggaaggcgt ggacgtgcgc ggcgacgcgc tgtcgctggt cgtgatcgac
     3301 aagctgccgt tctcgccgcc ggacgatccg gtgcttgccg cacgcatcga ggcaatggag
     3361 cgcaagggcc tgaacggctt cgtgcaccac cagctgcctg cggcgatcat caatctaaag
     3421 cagggtgcgg ggcgtctgat tcgcgacgag accaatcgcg gggtgctgat gatctgcgat
     3481 ccgcgcctca tctcgaaacc ctacggcagg cggatctggc agagcctgcc gccgttcagg
     3541 cgtacgcgcg agagggatgt cgcatgcgcg ttcctgcagc aggtcgacgc gcccgccgcg
     3601 tgagcggccg gcgcgcccgg tactacttgt agggttggat caccaccttc ccggtcacct
     3661 tgcgcgcggc catgtcgttc agggcgcgcg ctgtttcagc cagtccgtag cgcgccgaga
     3721 tatgcggccg gatcttgcct tgccccatcc actccatcag ttcccgcatc gccgccaggt
     3781 tggctttcgg ttcgcgcttg gcgaactcgc cccagaacac gccgaccacc gaggcgccct
     3841 tgagcagcgg aagattgagc ggcagcttgg gaatctcgcc gttggcgaag ccgatgacga
     3901 gatagcgtcc gcgccaggcg attgaacgga acgccggttc cgtgtactcg ccgccgaccg
     3961 ggtcgtaaat cacgttcgga cccttgccat cggtcgcggc cttgatcgct tcgcgcagat
     4021 cggtggtgct gtagttgatc gtgaggtccg cgccgtgttc gcggcacacc gccagcttgt
     4081 cgtcgctgga cgccgccgcg atcacgcgtg cgcccagcgc cttgcctatt tcaatcgccg
     4141 ccaggcccac gccgcccgcc gcacccagca ccagcatggt ctcgccagct ttcaattggg
     4201 cgcggtcgac caccgcatga tgcgaggttc cgtaagtgag cgtgaccgcc gcggccgtat
     4261 cgaaatcgag acctggaggc atcggcatcg ccgccgcggc cgggacggcg atctgctgcg
     4321 cgaacgcgcc ctggctcacg aaggccagta ccttgtcgcc gggcttgaga tgggtaacgc
     4381 cttcgcccac cgcgcgcacc acgccggcca gttcgctgcc cggcgtgaat ggcagctggg
     4441 gcttggcctg gtacttgttc tggatgatca gcacatcagg aaaattcacg cccgcggcct
     4501 gcacatcgat cgccacctgc cccgggcccg ggaccacgtc cggcaactct tcgacaacca
     4561 gcgaatcggg cggtccccat gctttgcaca ctacggcttt catgatgtct cctgatgttg
     4621 ggtttataaa aaagaacgac cgttccattt tagcgcaagt gtcgggccgc ctgttaggcg
     4681 atgcgatccc gcggtttgcg aaagtgttgc ttcgggtaaa atcggcgcca tgaaaatcct
     4741 ggttagtaac gacgacggct atcttgcgcc gggtttgatc gcccttgccg aggcgctcaa
     4801 gccgattgcc gacattgtcg tcgttgcacc ggacagcaac cggtccggca gctcgaattc
     4861 gctcacgctt gaccggccat tgtcggttta ccgggccgag aacggctttt acttcgtcaa
     4921 tggcacgcca tcggactgcg tccatatcgc gctcaccggg gtgctggatg aaaagccgga
     4981 ccttgtcgta tccgggatca atcagggaca gaacatgggc gacgacacct tgtattccgg
     5041 gacggtcgcc gccgccaccg aaggtttcct gttcggtatc cccgcgatcg cattctcgca
     5101 ggttgaaaag ggctgggcgc atctcgagtc ggccgcgcgc atcgcccggg aaatcgtgga
     5161 acgcagcgcc gatcgcggac ttggggcgct ggggcggccg tatctgctca acgtcaatat
     5221 tccgaacctg ccgtatgaac agatccccgg cttgcgtccg agccggctcg gcaagcgcca
     5281 cgagtccgaa gccgtgatca ggagcaccga tccgcacggg cgcgagatct actggatcgg
     5341 accggcgggc cgtgccaagg atgccgggga agggacggat ttccatgccg tgacgaacgg
     5401 ctatgtctcg attaccccgc ttcaggtcga cctcacccac accgagcagt tgcgcgcact
     5461 tcagcagggg ctggtatgaa cgccaaggaa aaacgctttc cgttatcgct ggactcggtc
     5521 gtcagcaaag cgcgggaagc cgccggctcg cgctcagcca gcagtggcac tgcccagacc
     5581 gccagtcgca atgcgcttgc tcaccagacc agggcgccgg tcccatccat ggaggcgccg
     5641 cgcgtggcgc cggtcgtgtc ggatgcggtg cgcggggcga tggtgggtcg cgtcgcgcag
     5701 cagggcgtga aggacaagaa ggtgcttgcc gcactggagg cgatcccgcg ccacctgttc
     5761 gtgcagccgg cgctggccag tcaggcctat atcgacgcgt ccttgccgat cgggcatcac
     5821 cagacgattt ctcagcccta tatcgtggcg cgcatgatcg aggtcctgcg cgacaacaag
     5881 gccggcggga ggctggaacg ggtgctggag atcggcaccg gctgcggcta tcaggcggcg
     5941 gtcctgtccc tggttgcggt cgaggtttac tcggtcgaac gcatcaagcc gctgcacgaa
     6001 ctggcgaagt cgaatctgcg tccgctgcgc atccctaata tccgattgca atacggagat
     6061 ggtatgcttg ggcttcccca ggcagctcct ttcgacggca tcatcctcgc cgccgccggt
     6121 ctcgaggtcc cgcaggctct tctcgagcag ttgagcatag gtggtcgctt ggttgcgccg
     6181 gttggagagc agcggcaggt cctgcagctc atcgaacgtg tcgcgcagcg cgaatggaag
     6241 acgaccaccc tggaagggtg ccatttcgtt ccgctgcgac ccggtgtcat ataggttttc
     6301 ccgcgcacgc ccttgcagac gtgcgcgcat ctgaatattg cataagcatg agaaagtcca
     6361 gtttcacggt tgttgccttc attcccgttc tgctcgctgc ctgcagtacc gccacccgtg
     6421 cgcccgctcc ggtgctcgac cgtaccagcg cggcgcgtcc ggtcgagaat ggacggccgg
     6481 gcgtggcggc caatcaggtg ccgaaacagg cggatgcgcg cggcttctac acggtcaagc
     6541 gcggcgacac gctgatccag atcgccctcg aattcggcca gaattatcgc gacctcgttg
     6601 tctggaacaa tcttgcaaac ccgaacgaca tcaaggtcga ccaggtcctg cgggtcttgc
     6661 cgccagagac aggagcgcag acagcgagcg tggccactac gccgggcgtc gaagtccgtc
     6721 cgctcgcgcc gggtgctgcc gccgggacca gcaagacctc gccgcgcggc gacaagcggc
     6781 cgtattccga cgccgcattg gcggaaatgc aaaagggtga aggcgagccc acgccttctg
     6841 cgcaggcaag cgctcctgct gcgagcgtgc cgggatcgcc ggcgcgtggg ccgcaaaatg
     6901 cagcggcacc gctgccggac gacgagaaca tttcctggat ctggcctgct gaaggccgcg
     6961 tgatttccca gtttggcgaa ggccgtcgcg gcatcgccat cgccggccag atgggccagc
     7021 cggtgctcgc agcgggctcc ggcaaggtct tgtacgcggg cagtggaatt cgcggatatg
     7081 gcaatcttgt catcgtcaag cacacgaaca acctgctatc ggcctatgcg cacaacaaaa
     7141 ccatctttgt taaagaagat caaacagtcg cgcagggaca aaagattgcg gagatgggaa
     7201 actccgacac tgacgaggtc aagcttcact tcgagattcg ccaccagggc aagccggtcg
     7261 atccctcgaa atttctgccc aacagatagc gctgacgata gagcacgatg aaccgcaagg
     7321 caccggattc catggatgac gatgagccga ccgacgattt gtcggatgca atggctcatg
     7381 gcgcagagga tggccttgca ccggacggcg agggcgctgt cgatgcaggc gagatcggtc
     7441 atgcgcccgc cgaacccgtc gacgagctca agaccgtgct ggctgccgag ctggcaaccg
     7501 atacgaccca gcattacctg aaccagatcg gtgcgcggcc attgctgacc gtcgccgagg
     7561 aagtgcatta cgcgacgctc gccaagcagg gcaacttcga ggctcgccag aagatgatcg
     7621 agcacaacct gcgactggtc gtctccattg ccaagcacta catcaatcgc ggggttgttt
     7681 tgctcgacct gatcgaggaa ggcaatctcg gcctgatgag ggcgatcgac aagttcgagc
     7741 ccgagcgcgg attccgcttc tcgacctatg caacctggtg gatacggcaa agcattgaac
     7801 gcgcaatcat gaaccaggcg cgcacggtcc gtctgccggt ccacatggtc cgcgagctga
     7861 accagatcct gcgcgcgaag taccatcttg aagcgcaaca tcacgacggc aaggaagctt
     7921 cggcggaaga cattgcccat ctggtcgatc gccaggtcga ggacgtgcag gacatcctcg
     7981 cgctgtccga gcatgcaacc tctctcgatg cgccgctcga caacgatccc caggccagcc
     8041 tgatggacat gctgcccggc gatgcagaag acgggccgga ccaccaggcc gagcatcacg
     8101 aaatgacggt gctggtgcgt gactggctga agaaattgcc ggacaagcag cgcatcgtca
     8161 tcgtgcgccg cttcggcctg gacaatgacg atccggccac gctggaagag ctggctgccg
     8221 aaatgagcgt cacgcgcgag cgcgtgcgcc agatccagca ggaagcactg gtcaaactca
     8281 agcgcgcatt gaccgcgcgt ggcgtgggca aggacgcgtt gttgtgatgc cggtcctggt
     8341 tttcgacatc gagacgattc ccgatgtggc aggactgcgc gcgctcaatg cctttcccgc
     8401 ctcgatgagc gacgccgagg tggctgcggc cgcttttgcc gcacgccgcg agaaaaccgg
     8461 ctcggatttc ctgcagcatc atctgcacag gatcgccgcg atttcctgcg tgttccgcga
     8521 taatgacggc tttcgcgtgc gctcgcttgg cgggctcgag gatggcgagc agaagctggt
     8581 ggcggacttc ttccgggtga tcgaccgcta cacgccacag ctggtctcct ggaacggcgg
     8641 cggattcgac ctgccggtgc tgcactaccg agcgctgctg catggcgtct ctgccgcacg
     8701 ctattgggaa atgggcgacg aggaccgcga cttcaagtgg aacaactacc tgagccgcta
     8761 ccatacccgc cacatggacc tgatggatct gctggcactc tataccggcc gggccaacgc
     8821 gccgctcgac gacctggcca agctgtgcgg cttcccgggc aagctcggcg tcgacggctc
     8881 ccaggtctgg aacctgtacc aggaaggccg gctcggcgaa attcgcgcat actgcgaaac
     8941 cgatgtcgtc aacacctatc tcgtgtattg caggttccag ctcatgcgcg gcagcctgag
     9001 catggaagcg tatcgcgagg aactttcgct ggtgcggcat tccctcggcg gactcgaggg
     9061 cgagcactgg aaggaatacc tgtccgcctg gccctcgcaa gcctgaccga ttccgctccg
     9121 cacgattccc cgttcacggc gcgatgccgc cactctcttt tccgtttatg cagcagaacg
     9181 tcattctcat cgaatccctc gacatggaag gccgcggtgt cggccaccag gtgaacgagg
     9241 acgggagtcc cggcaaggtc atcttcgtcg agggtgcatt gccgggcgag cgggtcgaat
     9301 accagtcgtt tcgcagaaag cccaaatggg aggccgcgac gctgacggcg ctccacaatg
     9361 aatcagcgct gcgcgtgcag cccaagtgtg tgcatttcgg cacctgcggc ggctgcgcga
     9421 tgcagcacct ggaaccgggc gcgcaggttg cggtcaagca gcgtgtcctg gaagacaatc
     9481 tctggcacct tggcaaggtg cgtgccgaga cgatgctcgc gccggtccac ggtccgacct
     9541 ggggctaccg ttaccgcgca cgcctgtcgg tgcgccatgt ggcgaagaag ggcggcatgc
     9601 tggtcggctt tcatgaacgc aaatcctcgt tcatcgccga catgacgagc tgccagatcc
     9661 tgccgccgca tgtgtcagac atgctggtgc ccttgcgcgg gctggtcgga tccctgtcga
     9721 tcttcgatcg catgccgcag atcgaactgg ccgtgggcga gggggtgacc gcgctggtgc
     9781 tgcgcaacat ggcgccgctg actacggacg acgaggacag gctgcgcgcg ttcgccgaca
     9841 cccatggcgt gcaatggtgg ttgcagccca aggggccaga cacggtgtac ccgttctatc
     9901 ccgagcaaag cacgctctgc tacacgcttc ccgagttcgg cgtgcgcatg ccgttcaagc
     9961 cgaccgattt cacccaggtc aatcaccaga tcaaccgggt gctggtatcg aaggcgctgc
    10021 gcctgctcga catgcagccg aacgagcggg tggcggatct gttctgcggg ctgggcaact
    10081 tcacgctgcc gctggccacc cgcgcggccg aggtggtcgg catcgaaggc agcaccgcgc
    10141 tgaccgagcg cgccctcgag aatgcccgac tgaacggact ggacacgaag acaagcttcc
    10201 actgccgcaa cctgttcgag gcgaccgccg ccgatttcgc tgcactcggg cgcttcgacc
    10261 gcatgctgat cgatccgccg cgcgaaggcg cgctggcggt ctgccaggcg ctggtcgagc
    10321 tgggcaaggc cgatgcgtcg atgcgaccgc gccgcatcgt ctacgtgtcc tgcaacccgt
    10381 cgacacttgc gcgcgacgcc ggatacctgg tgcaccaggg cggctacacg ctgcgccagg
    10441 cgggggtggt caacatgttc ccgcacaccg ctcacgttga atcgattgct gtcttcgacg
    10501 ccgcctgagg gtggggcagg gtggctttca tgctaaaatc gcgggttcct tggattctga
    10561 gaacaataac ttaacccttt ctttttattg gagtttttac agatggcaat cgagcgcacc
    10621 ctgtccatta tcaagccgga cgccgtggcg aagaacgtga tcggacagat ctacacccgt
    10681 tttgaaaatg ccggcctgaa gatcgtcgcc gcgcgcatgg cgcaactgtc gcgcgccgaa
    10741 gccgaaggct tctatgcggt gcaccgcgag cgtccgttct tcaaggatct ggttgacttc
    10801 atggtctccg gcccggtgat gatccaggtg ctcgaaggcg aaaacgcgat tgccaagaac
    10861 cgcgaactga tgggcgcaac cgatccgaag aaggccgaca agggcaccat ccgcgccgac
    10921 ttcgccgatt cgatcgacgc caacgctgtc cacggctccg acgccccgga caccgcgaag
    10981 gtcgaaatcg cctacttctt cccggccctg aacgtctatt cccgctaaga agatcgcgcc
    11041 gcttcctgcg gcgtgttgta cgcataccat gacggctctc accaatctgc tggacctcga
    11101 tcccgctcaa cttgtcgcct actgcgccga gctgggtgag aagccgtttc gcgccaagca
    11161 gctgcagcgc tggatacacc agttcggcgc cagcgacttc gacgagatga cggatctggc
    11221 gaagtcgctg cgcgaaaaac tgaagacgcg cgcgaccatt gccgcacccg ccatcatcag
    11281 cgatcacacc tcgtccgatg gcacgcggaa atggctggtc gatgtcggcc agggcaacgc
    11341 cgtcgaaacc gtattcatcc cggaagaaaa ccgcggcacg ctgtgcattt ccacgc
//
LOCUS       DATLGF010000238        14965 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_88939,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000238 DATLGF010000000
VERSION     DATLGF010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 14965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14965
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88939"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VM406_07185"
     CDS             complement(<1..293)
                     /locus_tag="VM406_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HVL75783.1"
                     /translation="MKTSFAVSTIVAAAFLAGCAGSGPRQAQSYPAPYPSSYPSYPVG
                     QPQGNPYAVQYGTVDSIQYRQAAGQGGVGMGTVVGGVVGGVLGNQVGSGSGRKA"
     gene            475..1374
                     /locus_tag="VM406_07190"
     CDS             475..1374
                     /locus_tag="VM406_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HVL75784.1"
                     /translation="MDNIASVPVSFPARLRALLMRSVEQWINDRAMSKGAALAFYTLF
                     SLAPVMLMVLAIAGAVFGEEAARGALFEELRGMIGPSGAEAAQLLLAQARNPQAGLAA
                     TMLAIGLLLVGATTVFGELKESLDEIWKVPPSKQSSIMTFIRTRLLSFSLILVLAFLL
                     LISLAVNAALAAAERYLAGYWGEAAAIMLPISSSISFGVIVVLFAAIFKLLPQVRLPW
                     RDVWFGALVTAALFQVGRLMIGAYLGNNEFVTSFGAASSIIAVMMWVYYSSLIFFFGA
                     ELTRQYSLLFGSRRRRPLREDPQ"
     gene            1412..1951
                     /locus_tag="VM406_07195"
     CDS             1412..1951
                     /locus_tag="VM406_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HVL75785.1"
                     /translation="MPRIPYQPLDLSEPAALVDAIRKRRGGTLLNLDRMLLHSPAFAS
                     GWNGFLGAVRTGLALSPKLRELAMCVVAVLNGAEYEFHHHAPEFIAAGGTEAQVAALR
                     EPERALANEALFDAAERAALRLTIEMTRSVKVADDTFAAVRNALGSEQLVVELVGVIA
                     TYNMVSRFLVALGVEPEQG"
     gene            2695..5718
                     /locus_tag="VM406_07200"
     CDS             2695..5718
                     /locus_tag="VM406_07200"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVL75786.1"
                     /translation="MNHGDRHGPGPALRSAALRPLVAGVLYWLAAAVSLPLAVPGAII
                     SPIWPAAALALFLSLRWGQRVIPAIGLGAALAFLTHGWPLPGSLIVGSAAALAAWGAC
                     SLICRMQQREGDFTYNGNLFVFILIALACSLITAPLAAAGAMTAGRLGPDPWLGPLTW
                     TLGTATGMIIFTPLLRAWRVNRGIDWTRGKLFEAAMFTALLLFSNYIAFTDWFGPLPL
                     SYLPVPFVIWAAYRFNVPAVAWTTALICLTAAWGTMRGAGPFAHADINSALLLLAVYV
                     SVLGATGLTLSSLLSERALAEGHLTRERDQLEQRVLERTDALRDELHERKRVEQQLAE
                     AQQVALIGSWNWELEERNIQWSDQLYRIYGIPLGTKPPGPRSYVYCIHPDDVPKVNEA
                     FVRCRDEGTPFSFEHRIVLPDGSVRVVAARGRGEKGPDGRVVRLFGTVQDITEAHEAE
                     LALKEAQERYRRVVEISPDAIVIQQGDEIVFANPASERLIGGPLDRPLIGRSILDLIH
                     PDARETFIQHAKTLQEGRNLVQAESAMLRVDGSRLDVEISATPFPHQGKLAVLYTMRD
                     ITERKRSAEQLAYLAHYDSLTGLPNRLMFHQQLEHALSVAARPHRTLEVLFLDLDRFK
                     QINDTLGHEAGDAVLKEAARRLRSSLRESDTVARLAGDEFVVLVENVDEPQRGGTIAE
                     KILSAFSRPFSLHDSALQIGTSIGIAAYPVDALDAATLVKRADEAMYRAKQARNSYCY
                     YSAHLNSQQAERRTLEQALRLAVERGEMTLHYQPRIDVASGNLVGMEALLRWTHPQLG
                     PVAPRHFLPLAEKSGLILPIGYWGLRLACVQTRQWQDGGAGKLKVAVNLSARQLADPA
                     LVENIRAILDETGLDAQALEVEITEDAMRAEPERAMQAIKTLHAAGVRVALDDFGTGY
                     SSLGRLKQIPFDAVKIDRSFIQGLPTDAADAAITRGIIQLGHSLQCHVVAQGAETQQQ
                     FDFLRAHACDSVQGFYFSEAMPPAHFNELVHAQFH"
     gene            complement(5687..6475)
                     /locus_tag="VM406_07205"
     CDS             complement(5687..6475)
                     /locus_tag="VM406_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HVL75787.1"
                     /translation="MILIGGRMAVAEFLFHAELNRFIAPALRARRFSHPCARNATVKH
                     MIEVFGVPHTEVGAVWINGVPVDFSTLLREGDRVEVRPWHASRNEERAMLRRAHGERF
                     VADAHLGQLARNLRMLGFDVLYRNDFSDAEVAALSATEDRIVLTRDRDLLIRKQIEHG
                     CFLHATHADEQVMEVLRRFRLADAVQAFSRCLSCNAPLQAAAPEEVAARVPPQSHASH
                     ERFFECVGCERVYWEGSHVVRMRARIAQWLERAQLSETARAPVR"
     gene            6562..7392
                     /locus_tag="VM406_07210"
     CDS             6562..7392
                     /locus_tag="VM406_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVL75788.1"
                     /translation="MAYKTILVHVDESQRASERIKIASAIAMAENAHLIGTAVTGASR
                     YLIQERALEVDPNLRRHLDDLRARAQHGLADFEACVQKLGLRSFEKRLVDDEAGAGIS
                     LQARYADLVVIGQYDPDEVSPVVMHDFPQYVVLNCGRPVLLVPHTGQFDNIGRRVLIG
                     WDASLASIHAASAALPLLQRAQQITAVTFGESEREKEAPPLDELQRYLARHGVNVDMQ
                     RREDSRGVGAAMLSLCAELDADLLVMGGYGHSRFREILLGGVTRAVLTEMAVPVLMAH
                     "
     gene            7489..8886
                     /locus_tag="VM406_07215"
     CDS             7489..8886
                     /locus_tag="VM406_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HVL75789.1"
                     /translation="METVAGMDATMLARIQFAFTVSFHIIFPAFTIGLSAWIATLEIM
                     WLRTGIQHYHTLARFWTKIFAVSFAMGVVSGIVLSYQFGTNWSRFSEVVGNTIGPLIG
                     YEVLTAFFLEASFLGVMLFGWNRVPPWLHTLSSVIVAFGTALSGFWILSANSWMHTPA
                     GFGIDENGITYPVSWFEIIFNPSFPYRFAHMMIAAYLTTSLVVLATGARWVRAGRFMD
                     EGATMVRMGLGMVAILAPLQLVVGDLHGLNTLEHQPAKIAAMEAHWDGSKVAPFVIFA
                     WPDEQQERNRYELSVPNVGSLILTHEWEGTFPGLLDFAPEDRPPVVPVFFAFRIMLTC
                     GFIMIGIGLIGTWLWVRKRLLTTEWFLKPVGHAWPLGFIAILAGWMVTEIGRQPWVAY
                     GILRTADAISPVAASTVATSLVMFIIVYGIVFSIGVIYIYRMLRRGPKLVPHEAQALP
                     NRPLSVAQNLGGEQP"
     gene            8883..9896
                     /gene="cydB"
                     /locus_tag="VM406_07220"
     CDS             8883..9896
                     /gene="cydB"
                     /locus_tag="VM406_07220"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HVL75790.1"
                     /translation="MSNLEFILPIIWALLIGVAVAMYVVLDGFDLGIGILFPFLRDES
                     ERDQAMNSVAPFWDGNETWLVLGGGGLFVAFPMAYAIIMPALYLPVIIMLLALIFRGV
                     AFEFRWVAKPNHHKWDLAFTWGSIVAAFSQGLVLGGLLQEITVVNGQFGGGAFDWFTP
                     FSVMCGISLVVGYALIGATWMIMKNSGELEQHARRMGIRLLFGLLICIAIVSLWTPLQ
                     IPRIAERWFTVPNFYLLSQVPLATLILAWLCWRGIRKGRTVVPFAAAVGLFLLALLGL
                     VVSNVPYVVPPTVTLWEAAAHPASQFFLLIGAVILLPMIIGYMVFVFWVFRGKVQPGE
                     GYH"
     gene            complement(9940..13083)
                     /locus_tag="VM406_07225"
     CDS             complement(9940..13083)
                     /locus_tag="VM406_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVL75791.1"
                     /translation="MGFYRRLLDNHPLANITFLVVLMLGVLSYVNMPRAQDPEINFNW
                     VSIVTVLPGASAADVEREITSPLEDAIRLVKDIRYISSNSREGISSILVRFNDLPERE
                     FDKRMVDLRREVQNKASAELPLEARDPVVMEITTSNGFPTAMVAIYSAVGGEHLRYLA
                     SQIKTDLEDVRGVDSLLVTGLADPQLHVEFDPRALEARGAHPGQVADAVIGWFRNTMG
                     GRVQVGDREWLLRLEGKTPEPELLAQIPVPNQTGYFSVDTVGHAARGTERVNQRVTYN
                     GQPAMIFAVTKQAQANTLDLVARLQDFVQERNEVLASQGVQLALIDDQTVPTREAIGV
                     MQTNALYGWILVILVCWVFLGSRIAFLVGLGIPFSLAATFFIVAATGSTLNLTVLLGV
                     VIALGMLVDDAVVVVESIYYRMQRGEETVHAVVNGVREVALPVLTSILTTCAAFLPLM
                     LLPGIVGKFMFVVPFVVTTALLVSLIEAYWILPVHVMTFKPNFERKSRAQLWRERFTH
                     AARVRYTRLLIKVLRWPKLSVGAVLLTMAGAGAMVGLGAVKVQFFAFDPLRFFYVNVD
                     MPAGVTLDRTHAEVERVQKLVEPAFADGEARSLTAVSGIKWTETEPLYGTQYGQVIVS
                     LNPRTGDMRETQEVVAALREQLAAYRGEARISFLELSGGPPTSKAINVKVRGDNYEQL
                     RRATDELMAVVAQIPGVKDLVDDDVPGRPELTLSLRREALTQAGLNAGYVARLVRLYG
                     DGETVASIRDQGEKVDVVVRAHPAELADIDQALSYRIPLPQGGSIALGQLVDAQMQES
                     KGFIRHYQLRRAITIEADLARDVEHTTDTVTANNRIRAAWEQMRTRYPGVDLDFTGEL
                     DDIQESLDSMGRLFAFGVGLIYLILAAQFRSYFQPFLILVTVPLAFVGVVLGLFVSGN
                     PLSLYTLYGVIALAGIAVNSAIVLIDAANSRLSTGMSVLHATIYAARRRLIPILITTT
                     TTIAGLFSLAVGLGGRSLIWGPVASSIVWGLGFSTLLTLFVIPLLFRAFMTPGWWRPS
                     RWRWVQRWRGTPAPARA"
     gene            complement(13092..14099)
                     /locus_tag="VM406_07230"
     CDS             complement(13092..14099)
                     /locus_tag="VM406_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVL75792.1"
                     /translation="MRAVLLFLVSLLASPLSAAPAYTVKSLEELAVYPQSRAVARVVP
                     ENESRIAAEVSARIQEIPVAVGQPVRKGDVLVRLDQSQYQLALEQASSQVELLSNRHK
                     LAQLQYEQARSLAESNFISSQALEQRRTELAVLESELKIARNSVSQAQLALRRTVLRA
                     PFPGVVRERLAGEGEMAAPSQPILTLVEHARSEVRAQVPLGDVPSLRKAREIAFRLGQ
                     ARHPVKIERITPLVDPHAQTREVILRMQETLAAGVAGELLWTSTTPYLPAAYVQQFRG
                     RLGVWVEEGGKPVFKPVPDAQTGRPVALDWPLDTRIVDDGRFALAGPALEAAPSAGNG
                     K"
     gene            complement(14223..>14965)
                     /locus_tag="VM406_07235"
     CDS             complement(14223..>14965)
                     /locus_tag="VM406_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HVL75793.1"
                     /translation="STVKQNAASAHEANQLAVSASDVATKGGAVVARVVDTMGSINNS
                     AKKIVDIIGVIDSIAFQTNILALNAAVEAARAGEQGKGFAVVAAEVRQLAQRSAAAAR
                     EIKALIDDSVEKVGIGAGLVDQAGTTMKEIVESVRRVTDIMGEISVASKEQTDGIEQV
                     NQAINQMDEVTQQNAALVEEAAAAAQSLQEQAAHLAQVVSVFRLDMVQTAGARPAPAA
                     VQSARVTSAVPVPPPLARARAADDWEEF"
BASE COUNT         2469 a         4997 c         4835 g         2664 t
ORIGIN      
        1 gccttcctgc cgctgccact gccgacctgg ttcccgagca ccccgccgac cacgccgccg
       61 accaccgtcc ccatgccgac gccgccttgg ccggccgcct ggcggtactg gatggaatcg
      121 acggtgccgt actggaccgc atagggattg ccttgcggct ggccgaccgg atagctcgga
      181 tagctcgacg gataaggtgc gggatagctt tgcgcctggc gcggcccgga accggcacag
      241 cccgcgagga aggctgcggc gacgatggtc gagacggcga atgaggtttt catgagatgt
      301 ccttctgagc gattgcaatg tgcccattta acgccacgcc gtttggcacg gccagtgtcg
      361 ctgcgtaagg gttgaaacag cttgcaaccg cccggccgag cccattgcgt gcatgcatcg
      421 gcttgcgcgg gctcaacggc ggaatcatcg ccgctcgcgc gctgtctttg cagtatggac
      481 aacatcgcct ccgttcccgt ctcgtttcca gcgaggctgc gcgccttgct gatgcgctcg
      541 gtcgagcagt ggatcaacga ccgcgcgatg agcaaggggg cggccctcgc cttctacacg
      601 ctgttctcgc tggcacccgt gatgttaatg gtgctggcga tcgccggcgc ggtattcggc
      661 gaggaagccg cccggggcgc gctgttcgag gaactgaggg gcatgatcgg gccgagcggc
      721 gccgaggccg cgcaactcct gcttgcgcag gctcgcaatc cgcaggccgg cttggcggcc
      781 accatgctcg cgatcggtct gctcctggtg ggtgccacta ccgtgttcgg cgaactgaag
      841 gaaagcctgg acgaaatctg gaaagtcccg cccagcaagc agtcgagcat catgaccttc
      901 atccgcacgc gcctgctgtc gttcagcctg atcctcgtgc tggccttcct gctgctcatc
      961 tcgctcgccg tcaatgcggc gctcgccgcg gccgaacgct acctggccgg ctactggggt
     1021 gaggccgccg cgatcatgct gccgatctcg tcctcgattt cgttcggcgt catcgtggtg
     1081 ctgttcgccg ccatcttcaa gcttcttccc caggtccggc tcccttggcg cgacgtctgg
     1141 ttcggcgcgc tggtcaccgc ggcgctgttc caggtcggcc gcctgatgat cggcgcctac
     1201 ctcggcaaca acgagttcgt caccagcttc ggcgccgcca gttcgatcat cgcggtaatg
     1261 atgtgggtgt actactcatc gctgatcttc ttcttcggcg ccgaactgac gcgacagtat
     1321 tcgctgctgt tcggcagccg gcgccgacgg ccgctccggg aggacccgca gtgaccggta
     1381 caatcgcagt cttttaacaa gcacaaccgc catgccgcgc ataccgtacc agccgctcga
     1441 tctatccgaa cccgccgccc tcgtcgacgc catccgcaag cgccgcggcg gcaccctgct
     1501 caatctcgac cgcatgctcc tgcacagccc ggccttcgcc agcggctgga acggcttcct
     1561 cggcgcggtc cgcaccggcc ttgcgctgtc gcccaagctg cgcgaactgg cgatgtgcgt
     1621 cgtcgccgta ctcaacggtg ccgagtacga gttccatcac catgcgcccg aattcatcgc
     1681 cgccggcggc accgaggcgc aggtcgcggc gctgcgcgaa cccgagcgag cgctggcaaa
     1741 cgaagcgctg ttcgatgcag cagaacgcgc ggccctgcgg ctgaccatcg aaatgacacg
     1801 ttcggtcaag gttgccgacg acaccttcgc cgcggtgcgc aacgcactcg gcagcgaaca
     1861 gctggtggtg gagttggtcg gcgtgatcgc gacctacaac atggtgtcgc gcttcctcgt
     1921 tgcgctcggc gtggagccgg aacagggcta gttcagcatt aagccgccgc gatcggcttc
     1981 catcgcggcg atccttcatg gcgcttgcag cgccattgcg ttacgaagtg cacacctccc
     2041 gccgcgcaat cacctggctg ccgctgaatt ggcagcgaca ccctacaccg gcatgctgag
     2101 tgacggacga agcggggcac cttgtgccgc aatgctcgtg cgattctgct tcatcaccaa
     2161 agccgaaggt tcagtcacac gaggctacac atcgttgcgc tgaacaagcc gaacaggcgt
     2221 cgccgggacc gggccacgac ccctcaccgc gcgcggcgcg ctcgccgcaa gagggcggcc
     2281 acctgcactg acgcgggttc ccgtttcgat ccgcgcatcg cgcggcaacc ggtcgacctg
     2341 gccatcgcgg tcttgtcgta ccgtgctggc ctcgccttct ttcgcgtcgt ggcgtgcggt
     2401 ttcctacgcc gtgacggcgc ttcgggtgcc cctcctgacc gatacctttc tgcgctgcgc
     2461 agctcgcggc gcagcgccgt agccgcatgg gtcgcgggtt gatcgaactc aagcgacttc
     2521 catcctgtaa tacgcatcgc tgcagcgcgg aaaacaatgg agattcagtc gacgcagatg
     2581 gaaccgttgg ttactcgccc gcgtcttagc agttattcaa ctgtccgggc gccgcccgtg
     2641 gcagtgcccg aatgagaact tccgtcacgg gcgtgtccgc ctgacaggag ccacatgaac
     2701 cacggcgacc gccatggacc tggtcctgct ctccgcagcg ctgcgctgcg tcctctcgtc
     2761 gccggcgtgc tctactggtt ggcggcggcg gtctcgctgc cgctggccgt accgggggcg
     2821 atcatcagcc cgatctggcc ggccgccgcc cttgccttgt tcctgagcct gcgctggggc
     2881 cagcgggtga ttcccgcgat cggcctgggt gccgcgctcg ccttcctcac ccacggctgg
     2941 cccctgcccg gctcgctcat cgtcggctcg gcggccgcgc tggcggcatg gggggcctgc
     3001 tcgctgatct gccgcatgca gcaacgcgag ggcgacttca cctataacgg caatctgttc
     3061 gtattcattc tcatagcgtt ggcatgcagc ctgatcaccg ctccgctggc tgcggccggc
     3121 gccatgaccg ccggccgcct ggggccggat ccatggctgg gcccgttgac ctggacgctc
     3181 ggtacggcaa ccgggatgat catcttcact cccctgctgc gtgcgtggcg cgtcaaccgc
     3241 ggaatcgact ggacgcgcgg caaactgttc gaggcggcga tgttcaccgc gctgctgctg
     3301 ttttcgaact acatcgcatt caccgactgg ttcggaccac tgccgttgtc ctatctgccc
     3361 gtgcccttcg tgatctgggc cgcgtaccgc ttcaacgtac ccgccgtggc gtggacgacc
     3421 gcgctcatct gcctgaccgc cgcctggggc acgatgcgcg gcgccgggcc gttcgcgcat
     3481 gccgacatca actccgccct gctgctgctt gccgtgtatg tgagcgtact gggggcaacc
     3541 gggctgacgc tttccagcct gctgagtgaa cgcgccctgg cggaaggaca tctgacgcgg
     3601 gagcgagacc agctcgagca gcgcgtactg gaacgcaccg atgcgctgcg tgacgaactg
     3661 cacgagcgca aacgggtgga acagcaactc gccgaggcgc aacaggttgc actgatcgga
     3721 agctggaact gggaactgga ggagcgcaac atccagtggt cggaccagct gtacagaatc
     3781 tacggcattc ctctcggcac gaagccgccc ggtccacgtt catatgttta ctgcatccat
     3841 ccggatgatg ttccaaaggt taacgaggcg ttcgttcgct gccgcgacga aggcacgccg
     3901 ttttccttcg aacaccggat cgtgctgccg gacggcagcg tacgcgtggt cgccgcgcgc
     3961 ggccgcgggg agaaagggcc ggacggccgc gtggtgcgtc tgttcggcac ggtgcaggac
     4021 atcacggaag cgcacgaggc ggaactggcg ctgaaggagg cgcaggagcg ttaccggcgc
     4081 gtggtcgaga tttctccgga tgccatcgtg atccagcagg gcgatgaaat cgtgttcgcc
     4141 aatccggcaa gcgagcgtct gatcgggggc cccttggacc ggccgctgat cggtcgctcg
     4201 atactcgatc tgatccaccc cgatgcgcgt gagacgttca tacagcacgc gaagacgctg
     4261 caggaaggac gcaatcttgt tcaggcggaa agcgcaatgc tgcgcgtcga tggaagccgg
     4321 ctcgacgtcg aaatcagcgc cacgccgttt ccgcatcaag gcaagctggc ggtgctgtac
     4381 acaatgcgcg atatcaccga gcgcaagcgc agtgcggagc agctcgccta cctggcgcac
     4441 tacgattcgc tgaccggcct gccgaaccgg ctgatgttcc accagcagct cgagcacgca
     4501 ctgagcgtgg ccgcgcgccc gcaccgcacg ctggaggtgc tgtttctcga tctggaccgt
     4561 ttcaagcaaa tcaatgacac gctcggtcac gaagcaggcg acgcggtgct gaaggaggcc
     4621 gccaggcgcc tgcgctcaag cctgcgcgag tcggataccg tggcgcgact ggccggcgac
     4681 gagttcgtgg ttctggtgga aaacgttgat gagccgcagc gcggcggcac catcgcggag
     4741 aagattctgt cggcgttttc ccggccgttc tcgctgcatg acagcgcgct tcagatcggc
     4801 acgagcatcg gcatcgccgc ctatccggtc gatgcgctcg acgcggccac cctggtcaag
     4861 cgggccgacg aggcgatgta ccgcgccaag caggcgcgca acagctactg ctattactcg
     4921 gcacatctga acagccagca ggcagagcgg cgcacgctgg aacaggcgct gcggcttgcc
     4981 gtcgagcgcg gcgaaatgac gctgcactat cagccccgca tcgacgtggc gagcggaaac
     5041 ctcgtcggca tggaggcgct gctgcgctgg acccacccgc agctcggccc ggtggcacca
     5101 cggcattttc tgccgctggc agagaagagc gggctgatcc tgccgattgg ctactggggc
     5161 ctgcgcctgg cctgcgtgca gacgcggcaa tggcaggacg gcggcgcggg caagctgaag
     5221 gtcgccgtga acctgtcggc gcgccagctt gccgatccgg ccctggtcga gaacatacgt
     5281 gcgattctgg acgagaccgg gctcgacgcg caggcactcg aggtcgagat caccgaagac
     5341 gccatgcgcg cggagccgga acgtgcgatg caggcgatca agaccctgca tgctgcgggc
     5401 gtgcgcgtcg ctctcgacga tttcggcacc ggttactctt cgctcggacg gctcaagcag
     5461 attccgttcg acgcggtgaa gatcgaccgc tccttcatcc agggcttgcc cacggacgcg
     5521 gccgatgccg ccatcacgcg gggcatcatc cagctcggac atagcctgca gtgccacgtg
     5581 gtggcacagg gcgcggaaac gcagcagcag ttcgatttcc tgcgcgcgca tgcgtgcgac
     5641 agcgtgcagg gcttctactt cagcgaggca atgccgccgg cccacttcaa cgaactggtg
     5701 cacgcgcagt ttcactgagc tgcgcgcgct ccagccattg cgcaatacgc gcgcgcatgc
     5761 gcaccacgtg cgagccttcc cagtacacgc gctcgcagcc gacgcattcg aagaagcgct
     5821 cgtggctggc atggctttgc ggcggcacgc gtgcggccac ctcctccggc gcggccgcct
     5881 gcagcggcgc gttgcagctc aggcatcgcg aaaaagcctg taccgcatcg gcaaggcgaa
     5941 agcgccgcaa gacctccatc acctgctcgt cagcatgtgt cgcatgcaga aagcagccat
     6001 gctcgatctg ctttcggatc agcaagtcgc ggtcgcgcgt gagcacgatg cggtcctcgg
     6061 tcgcggacag cgcggcaact tcggcatcgc tgaagtcgtt gcggtagagc acgtcgaaac
     6121 caagcatgcg caggttgcgc gcgagctggc cgaggtgggc gtcggcgacg aagcgctcgc
     6181 catgcgcgcg gcgcagcatg gcccgctctt cgttgcggct ggcgtgccag ggacgcacct
     6241 cgaccctgtc cccttcccga agcagcgtgg agaaatccac cgggacgcca ttgatccata
     6301 ccgcaccgac ctcggtatgc ggcacgccga agacctcgat catgtgcttg accgtggcgt
     6361 tgcgcgcgca gggatgcgag aaacgccgcg cccgcaaggc cggcgcaatg aaacggttca
     6421 gctccgcgtg aaaaagaaac tccgcgacgg ccatgcgacc cccgatcaaa atcattgaga
     6481 cagctcaact gcgccgccgg tgcccgtcgg ttgacacgaa aaaaagctgt ctaaagccat
     6541 gttcgcgcaa tgggaggccg catggcgtac aagacgatac tggtgcatgt ggatgaatcg
     6601 cagcgtgcca gcgaacgcat caagatcgcg tccgccatcg ccatggccga gaatgcccac
     6661 ctgatcggca ccgccgtcac cggcgcctcg cgctacctga tccaggagcg cgcgctggaa
     6721 gtcgacccga acctgcgccg tcatctggac gatctgcgcg cccgcgcaca acacgggctc
     6781 gccgacttcg aggcgtgcgt gcagaaactg ggcctgcggt cgttcgaaaa gcggctggtc
     6841 gacgacgagg ccggcgccgg catttccttg caggcgcgct acgcggatct cgtggtgatc
     6901 ggccagtatg acccggacga agtctcgccg gtggtcatgc acgattttcc gcagtacgtg
     6961 gtactcaact gcggccgccc cgtcctgctc gtgccgcata ccggccagtt cgacaacatc
     7021 ggacggcgcg tgctgatcgg ctgggacgcg agtcttgcct cgatccatgc cgccagcgcg
     7081 gcgctcccgc tgctgcagcg tgcgcaacag atcaccgccg tcaccttcgg ggaaagcgag
     7141 cgcgagaagg aagccccccc gctggacgaa cttcagcgct acctcgcaag gcatggcgtc
     7201 aatgtggaca tgcagcggcg cgaagactcg cgcggcgtgg gtgcggcgat gctgtcgctg
     7261 tgcgcggagc tggatgccga tctgcttgtg atgggcggat acgggcactc gcgcttccgc
     7321 gagatcctgc tcggcggcgt gacccgtgcc gttctcaccg agatggcggt gccggtcctg
     7381 atggctcact gacccatccc gtcagccaag ctttgcttga gcaagctcac gacgggcccc
     7441 gccttgttcg gggtacacat ggagtttgcc cccctaccgg agtgaacgat ggaaacagtc
     7501 gccggcatgg acgcgacgat gctcgcccgc atacagttcg cattcaccgt cagcttccac
     7561 atcatctttc ctgcgttcac gatcggcctg tctgcctgga tcgccacgct ggagatcatg
     7621 tggctgcgca ccggcattca gcactatcac acgcttgcgc gcttctggac caagatcttc
     7681 gcggtgtcgt ttgcgatggg cgtcgtctcc ggcatcgtgc tgtcctatca gttcggcacc
     7741 aactggagcc gcttctccga agtagtgggc aacacgatcg gcccgctgat cggctacgag
     7801 gtgctgaccg cgttcttcct cgaagccagt tttctcggcg tgatgctgtt cggctggaac
     7861 cgcgtgcccc cgtggctgca tacgctgtca tccgtcatcg tcgcgttcgg cacggcgctg
     7921 tcgggcttct ggattctttc cgccaacagc tggatgcata cgccggcggg gttcggcatc
     7981 gacgaaaacg gcatcaccta cccggtttcg tggttcgaga tcatcttcaa cccgagcttc
     8041 ccctaccgct tcgcgcacat gatgatcgcg gcctacctga ccacgtcgct ggtggtgctg
     8101 gccaccggtg cacgctgggt gcgcgccggc cgcttcatgg acgagggagc cacgatggtg
     8161 cgcatgggac tgggcatggt ggccatcctc gccccgcttc agctcgtggt cggcgacttg
     8221 cacgggctca atacgctgga gcaccagccg gccaagatcg ccgcgatgga ggcgcactgg
     8281 gatggctcca aggttgcgcc gttcgtgatc ttcgcctggc ccgacgagca gcaggagcgc
     8341 aatcgctacg agctgtcggt gcccaacgtc ggcagcctga tcctgaccca tgagtgggag
     8401 ggaaccttcc ccggcctgct cgacttcgcg ccggaggacc gccccccggt ggtaccggtg
     8461 ttcttcgcgt tccgcatcat gctcacctgc ggcttcatca tgatcgggat cgggctgatc
     8521 ggcacctggc tgtgggtgcg caagcgcctg cttaccaccg aatggttcct caaaccggtc
     8581 ggccatgcct ggccgctcgg attcatcgca atcctggccg gatggatggt gaccgagatc
     8641 ggccgccagc cctgggtcgc gtacgggatc ctgcgcacgg ccgatgcgat ctcgccggtg
     8701 gcggcatcga cggttgcgac gtcgctggtg atgttcatca tcgtttacgg catcgtgttt
     8761 tcgatcgggg tgatctacat ctaccggatg ctgcggcgcg gaccgaagct ggtgccgcac
     8821 gaggcgcagg cgctgccgaa ccggccgctg tcggtggcgc agaacctggg cggggagcag
     8881 ccatgagcaa cctcgaattc attctcccga tcatctgggc gctcttgatc ggcgtggccg
     8941 tggcgatgta cgtggtgctg gacggtttcg atctcggcat cggcatcctg tttcctttcc
     9001 tgcgcgatga gtccgagcgc gaccaggcga tgaattccgt cgccccgttc tgggacggca
     9061 acgaaacctg gctggtgctg ggcggcggcg gcctgttcgt cgccttcccg atggcctacg
     9121 cgatcatcat gccggccctg tatctgccgg tgatcatcat gctgctcgca ctgatcttcc
     9181 gtggcgtggc tttcgagttc cgctgggtcg ccaaaccgaa ccatcacaaa tgggacctgg
     9241 cctttacctg gggctcgatc gtggcggcgt tttcgcaagg cctggtactc ggcggccttc
     9301 tgcaggagat caccgtggtg aacggccagt ttggcggcgg ggcgttcgac tggttcacgc
     9361 cgttcagcgt gatgtgcggg atttcgctgg tggtcggcta tgcgctgatc ggcgcgacct
     9421 ggatgatcat gaaaaactcc ggcgaactgg agcagcacgc gcgccgcatg gggatccgtt
     9481 tgctgtttgg cctgctgatc tgcattgcga tcgtcagcct gtggacgccg ctgcagattc
     9541 cgcgcatcgc cgagcgctgg ttcacggtgc ccaattttta tctcctgtcg caggtgccgc
     9601 tggcaacgct gatcctcgcc tggctgtgct ggcgcggtat ccgcaagggt cgcactgtcg
     9661 tgccgttcgc cgctgcggtc ggactgttcc tgctggccct gctcggcctg gtcgtgtcga
     9721 acgtgcctta tgtggtaccg ccgacggtta cgctgtggga ggcggccgcc cacccggctt
     9781 cgcagttctt cctgctgatc ggcgcggtga tcctgctgcc gatgatcatc ggctacatgg
     9841 tgttcgtgtt ctgggtattc cggggcaagg tccagcctgg cgaggggtat cactgaacgg
     9901 gaatggccgg cttcggccat ccgtcgcgcg ccctctcgct caggcccgtg caggtgcagg
     9961 cgtaccgcgc cagcgctgca cccagcgcca gcgcgagggc cgccaccacc ccggcgtcat
    10021 gaacgcgcgg aacagcagcg ggatcacgaa cagcgtgagc agcgtcgaga agccgagccc
    10081 ccagacgata gacgaagcga ccggccccca gatcagcgag cggccgccca agccgaccgc
    10141 cagcgagaac aggccggcga tcgtcgtcgt ggtcgtgatc agaatcggaa tcagccgccg
    10201 gcgcgccgcg tagatggtcg cgtgcagcac gctcatcccg gtactcaagc gtgaattggc
    10261 ggcgtcgatc aggacgattg ccgagttcac cgcaatcccc gcgagcgcaa tcacaccata
    10321 aagcgtgtaa aggctgagcg ggttgcccga tacgaacagg ccgagcacca cgccgacgaa
    10381 ggccaacggc acggtcacga gaatcaggaa cggctggaaa tagctgcgga actgcgcggc
    10441 caggatcagg tagatcaggc cgacgccgaa cgcaaacagc cggcccatcg aatcgaggct
    10501 ttcctggatg tcgtcgagct cgccggtgaa atcgaggtcg acgccgggat agcgcgtgcg
    10561 catctgctcc cacgcggcgc gaatgcggtt gttcgcggtg acggtgtcgg tggtgtgctc
    10621 cacatcgcgc gccagatccg cctcgatggt gattgcccgg cgcagctggt aatggcggat
    10681 gaatcccttg gactcctgca tctgcgcgtc gaccagctgg ccgagcgcga tgctgccgcc
    10741 ttgcggcaac gggatgcgat aggacagtgc ctggtcgata tcggccagct ccgccggatg
    10801 cgcacgcacg accacatcca ctttctcgcc ctggtcgcgg atgctcgcca cggtttcgcc
    10861 gtcgccatac aggcgcacca ggcgtgccac atagccggca ttgagtccgg cctgcgtgag
    10921 cgcttcacgc cgcagcgaca gtgtcagctc gggacggccg ggcacgtcgt catcgaccag
    10981 gtccttcaca cccgggatct gcgcgaccac cgccatcagc tcgtcggtcg cgcggcgcag
    11041 ctgctcgtaa ttgtcgccgc gcaccttcac gttgatcgcc ttcgacgtcg gcgggccgcc
    11101 cgacagctcc aggaagctga tgcgcgcttc tccccggtag gcggcgagct gctcacgcag
    11161 cgcggcgacc acttcctgtg tttcgcgcat gtcgccggtg cgcgggttca gtgacacgat
    11221 cacctggcca tactgcgtgc cgtagagcgg ctcggtctcg gtccacttga tgcccgacac
    11281 cgcggtcagc gagcgcgcct cgccgtcggc aaacgccggt tcgaccagct tctgcacccg
    11341 ctccacctcc gcgtgagtcc ggtccagcgt cacgccggcg ggcatgtcca cgttcacata
    11401 gaagaagcgc agcggatcga acgcgaagaa ctgcaccttg accgcgccga gacccaccat
    11461 cgcgccggca cccgccatcg tcagcagcac cgcaccgacc gacagcttcg gccagcgcag
    11521 caccttgatc agaagccgcg tgtagcgcac gcgcgcggcg tgggtgaaac gctcgcgcca
    11581 gagctgggcg cgcgacttgc gctcgaagtt gggcttgaac gtcatcacat gcaccggcag
    11641 gatccagtac gcttcgatca ggctgaccag cagggcggtg gtgacgacga aggggacaac
    11701 gaacatgaac ttgccgacga tacccggaag cagcatcagc ggcagaaagg ccgcacaggt
    11761 ggtcaggatg gaagtgagca ccggcagcgc cacctcgcgc acgccgttga ccacggcatg
    11821 gacggtttcc tcgccgcgct gcatgcggta gtagatcgat tcaaccacga ccacggcatc
    11881 gtcgaccagc atgccgagcg caatcaccac gcccagcagc acggtcagat tcagcgtgga
    11941 gccggtggcg gcgacgatga agaaggtggc cgccagcgag aacgggatgc cgagtccgac
    12001 caggaaggca atgcgcgatc cgaggaagac ccagcacacc aggatcacca ggatccagcc
    12061 gtacagcgca ttggtctgca tcacgccgat cgcctcgcgc gtgggcacgg tctggtcgtc
    12121 gatcagcgcg agctgaacgc cctggctggc gagcacctcg ttgcgttcct ggacgaagtc
    12181 ctgcagccgc gccaccaagt ccagcgtgtt ggcctgcgcc tgtttggtca ccgcgaagat
    12241 catggccggc tggccgttgt aggtcacgcg ctggttcacg cgctcggtgc cgcgcgcggc
    12301 gtggccgacg gtgtcgacgg aaaagtagcc ggtctggttc ggtaccggga tctgagccag
    12361 cagctccggc tccggcgtct tgccttccag gcgcagcagc cattcgcggt cgccgacctg
    12421 cacccgtccg cccatggtgt tgcggaacca gccgatcacc gcgtcggcca cctggccggg
    12481 atgcgcgccg cgcgcctcga gcgcgcgcgg atcgaactcc acgtgcagct gcgggtccgc
    12541 gaggccggtc accagcagcg aatcgacgcc gcgcacgtcc tccaggtcgg tcttgatctg
    12601 cgaggcgagg tagcgcagat gttcaccacc cacggcgctg tagatcgcca ccatcgcggt
    12661 cggaaaaccg ttggaggtgg tgatttccat caccaccgga tcgcgcgctt cgagcggcag
    12721 ttccgcgctg gccttgttct gcacctcgcg gcgcaggtcg accatgcgct tgtcgaactc
    12781 gcgctcgggc agatcgttga agcgcaccag gatcgaggag atgccttcgc ggctgttgct
    12841 cgaaatgtag cggatgtcct tgaccaggcg gatcgcgtcc tccagcggag aggtgatctc
    12901 gcgctcgacg tcggccgccg aggcgccggg caggacggtg acgatcgaca cccagttgaa
    12961 gttgatctcc gggtcctggg cgcgcggcat gttcacgtag gacagcacgc cgagcatcag
    13021 caccaccagg aaggtgatgt tggccagcgg gtggttgtcc agcaggcggc ggtagaagcc
    13081 catgatcgcg cctacttacc gttgccggcg gacggcgccg cctcgagcgc agggccggcc
    13141 agcgcgaagc ggccgtcgtc gacgatgcgc gtgtcgagcg gccagtcgag cgcgacgggg
    13201 cggccggtct gcgcgtcggg caccggcttg aacaccggct tgcccccctc ttccacccac
    13261 acgccgagac gccccctgaa ctgctggacg taggcggcgg gcagataggg cgttgtgctg
    13321 gtccaaagga gttcaccggc cacgccggcg gccagggtct cctgcatgcg cagaatcact
    13381 tcacgcgtct gcgcgtgcgg atcgaccagc ggcgtgatgc gttctatctt gaccggatgg
    13441 cgcgcctggc ccagccggaa cgcaatttcg cgcgccttgc gcaggctggg cacgtcgccc
    13501 agcggcacct gcgcccgcac ctcgctgcgc gcatgttcga ccagcgtgag gataggctgg
    13561 ctgggcgccg ccatctcgcc ctcccccgcc aggcgctcgc gcacgacccc ggggaatggt
    13621 gcgcgcagca cggtgcgccg cagcgcgagt tgcgcctggc tcacgctgtt acgcgcgatc
    13681 ttcagctcgc tttccagtac cgccagttcg gtgcggcgct gttcgagcgc ctgcgaggag
    13741 atgaagttgg attccgccag cgaacgcgcc tgctcgtact gcagctgcgc gagcttgtgc
    13801 cggttcgaca gcagctcgac ctggctgctg gcctgctcga gcgcgagctg gtactggctc
    13861 tggtccaggc gcaccagcac gtcgcccttg cgcaccggct gtccgacggc gaccggaatt
    13921 tcctgaatgc gcgcgctgac ctcagccgcg atgcggcttt cattttccgg aaccacccgc
    13981 gcgaccgcgc gcgactgcgg gtagaccgcc agctcttcga gcgacttgac cgtataggcg
    14041 ggcgcagcgg aaaggggaga tgcaagaagc gaaacgagga agaggaggac ggcgcgcatg
    14101 ctcggctttc acgatttgca caaaccggaa agcataacaa aaaagacaac ccgtcacgcc
    14161 cgtttttcgg ccggtgatgc ctgcgccaat tttcctgtgc agcggactaa cgcctcatca
    14221 cattagaatt cctcccagtc atcggcagca cgggcccggg caagcggcgg tggcaccggc
    14281 acggcgctcg tcacgcgcgc ggactggacg gccgccggcg ccggtcttgc gccggcggtt
    14341 tgcaccatat cgagtcgaaa cacgctgacc acctgtgcca gatgcgccgc ctgctcctgc
    14401 agcgactgcg ctgcggcagc agcctcctcg accagcgcgg cattctgctg cgtaacctcg
    14461 tccatctggt tgatggcctg gttgacctgc tcgataccgt cggtctgttc cttgctggcg
    14521 accgagatct cgcccatgat gtcggtcacc cgccgcacgc tctcgacgat ttccttcatc
    14581 gtggtgccgg cctggtcgac cagcccggcc ccgatgccga ccttctccac ggaatcgtcg
    14641 atcagcgcct tgatttccct tgcggcggcc gccgagcgct gcgcgagctg gcgcacttcg
    14701 gccgctacca ccgcgaagcc cttgccctgc tcgccggcac gcgccgcttc gacagccgcg
    14761 ttcagcgcga ggatgttggt ctgaaacgca atgctgtcga tcacgccgat gatgtcgacg
    14821 atcttcttcg ccgagttgtt gatcgaaccc atcgtatcca ccacgcgggc gaccaccgcg
    14881 ccgcccttgg tcgccacgtc cgatgcggag accgcgagct ggttcgcttc atgggcgctg
    14941 gcggcgttct gcttgaccgt ggagg
//
LOCUS       DATLGF010000239         3837 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_5143,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000239 DATLGF010000000
VERSION     DATLGF010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5143"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VM406_07240"
     CDS             complement(<1..394)
                     /locus_tag="VM406_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HVL75794.1"
                     /translation="MSAPTTVRGACPHDCPDTCALLITVQDGKAVAVKGDPDHPTTAG
                     VLCTKVARYTERTYHPDRLLHPMKRVGAKGEGRFERISWDEALDTIAARLKDIAVRDA
                     QAILPYSYAGTMGLVQGESMAARFFHKLG"
     gene            complement(459..1631)
                     /gene="argE"
                     /locus_tag="VM406_07245"
     CDS             complement(459..1631)
                     /gene="argE"
                     /locus_tag="VM406_07245"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HVL75795.1"
                     /translation="MTAAPDLSRPSADVLAMVERLVAYPTVSRDSNLGLIEWVRDYLA
                     AKGVRSRLTYDASGTKANLFATLGEGKKPGLILSGHTDVVPVDGQAWDSDPFKATLVG
                     DRLYGRGTADMKGFIGTALALVPQFLEADLDSALHLALSYDEEVGCIGVRGLIADLQE
                     IGLKPAGCIVGEPTSMRPIIAHKGTHRFRCCVTGREAHSSYTTLGVNAIEYAARIIVY
                     IRQMADRLAQLETRDYAFTVPYSTMQTGVVRGGIASNIVPKECEFQFEARTLPGVEAE
                     ELYREVRDYAATLLPEMQKVEPNAAIEFEWLASAPGLSMEESAALVQFAASLARERPN
                     GAVSYGTEAGLFQRAGIPTVVCGPGSIEQAHRPNEYVELEQLARCEAFMRRLMRRA"
     gene            complement(1641..2645)
                     /locus_tag="VM406_07250"
     CDS             complement(1641..2645)
                     /locus_tag="VM406_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL75796.1"
                     /translation="MSAGKSALLEMRHVSKRFVKSLDAAAKIANVFGANQKEEVVHAV
                     DDVNLVVSKGEVVGLVGESGCGKSTLGRMAVGLHGLSSGTRLWKGQEIDKLDPGERRK
                     KQLAIQMIFQDPYASLNPRLRVQDIVGEAPVAHGIVRSAEQREYVEGLLQRVGLDPAT
                     LRRFPHQFSGGQRARVGIARALAVKPEFLVCDEAVAALDVSIQAQVLNLFIKLRDELD
                     LTYLFISHDLGVVRHLSDRVVIMYLGRVVESAPAAEVFARPNHPYTAALLASAPKLEV
                     KKTEFVAIKGEIPSPLNPPSGCHFHPRCPHAMPRCKEERPALREVASMHMSACHLNDT
                     "
     gene            complement(2642..3637)
                     /locus_tag="VM406_07255"
     CDS             complement(2642..3637)
                     /locus_tag="VM406_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL75797.1"
                     /translation="MTTQYDGPTLVVEGLKTQFFTRAGVAKAVDDVSFTVKRGEIMGL
                     VGESGSGKSMTGYSIMGLIDSPGKVVDGKILLKGQDLRGLPQDAMRKIRGSRIAMIFQ
                     DPMMTLNPVLRIDTQMMEAVFAHQDVSRERAREMARDALARVGIPSPDERLRAYPHQF
                     SGGMRQRVAIAIALLNKPDLIICDEPTTALDVTIQGQILYEMQKLCRESGTALIWITH
                     DLSVVAGLADSVCVMYAGKIVESGTVRQVLEHPRHPYTYGLIGSAPSRNVHGQPLRQI
                     PGMTPSLLHLPTGCAFRTRCDRATEVCTAYPELRQVDGRALRCFHPVREVLEAVQ"
     gene            complement(3648..>3837)
                     /locus_tag="VM406_07260"
     CDS             complement(3648..>3837)
                     /locus_tag="VM406_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL75798.1"
                     /translation="GLGLPVTEPSLGLLIANGFQYLMSGKYWISFFPGIALLVTVVAI
                     NLVADQLRDVLNPRLQTQ"
BASE COUNT          667 a         1273 c         1230 g          667 t
ORIGIN      
        1 cgccaagctt gtgaaagaag cgcgcggcca tcgattcgcc ctgcacgagg cccatcgtgc
       61 cggcatagct gtatggaagg atggcctggg cgtcgcgcac ggcgatatcc ttcagccgcg
      121 ccgcgatggt atcgagcgcc tcgtcccagg aaatgcgctc gaaacgtccc tcgcctttcg
      181 ctcccacccg tttcatcggg tgcaacaggc ggtccggatg ataggtgcgt tcggtgtagc
      241 gcgcgacctt cgtgcagagt acgccggccg tggtcgggtg atccgggtcg cccttgacag
      301 cgacggcctt gccatcctgg accgtgatca ggagcgcgca ggtatcgggg cagtcgtgcg
      361 ggcaggcgcc gcggacggtg gtgggggcgg acatgttcga cctttcgtcg gaaccggggc
      421 aatacgctat tttgaacgat tggacgcggc gcagcagatc aggcgcgccg catcaggcgc
      481 cgcatgaagg cttcacagcg tgccagctgt tcgagctcga catactcgtt cgggcggtgc
      541 gcctgctcga tgctgccggg cccgcacacg accgtcggta tcccggcccg ctggaacagg
      601 cccgcctctg tcccgtagga aaccgcgccg ttaggccgct cacgcgcgag cgacgcggcg
      661 aactgcacca acgcggcgct ttcttccatg cttaagccgg gcgcagaggc cagccactcg
      721 aactcaatcg cggcattcgg ttccaccttc tgcatctccg gcagcagcgt ggccgcatag
      781 tcgcgcacct cgcggtacag ctcctccgcc tccacgcccg gcagggtgcg cgcctcgaac
      841 tggaactcgc attccttggg cacgatgttg gatgcgatgc cgccacgcac gaccccggtc
      901 tgcatcgtcg aatacggaac cgtgaacgcg tagtcgcgcg tctcgagctg ggccaggcga
      961 tcggccatct ggcggatgta gacgatgatg cgcgccgcgt actcgatcgc gttgacgccc
     1021 agggtcgtat agctggaatg cgcctcgcgc cccgtcacgc agcagcgaaa gcggtgtgtg
     1081 cccttgtgcg cgatgatcgg ccgcatcgag gtcggctcgc cgacgatgca gccagccggc
     1141 ttgagcccga tctcctgcag gtccgcgatc agtccgcgca cgccgatgca gcccacctcc
     1201 tcatcgtacg aaagcgccag atgcagcgcg gaatcgaggt ccgcctcgag gaattgcgga
     1261 acgagcgcga gcgcggtccc gatgaagcct ttcatgtccg cagtgccgcg gccgtagagg
     1321 cgatcgccaa cgagcgtcgc cttgaacggg tcgctgtccc aggcctggcc gtcgaccggc
     1381 acaacatcgg tatggccgga caggatcagc cccggcttct tcccttcgcc gagcgtggcg
     1441 aacagattgg ccttcgtgcc gctggcgtca taggtgagcc gggagcgcac accctttgcg
     1501 gcgaggtaat cgcgcaccca ttcgatcagg ccgagattgg agtcgcgcga aacggtcgga
     1561 taagccacca gccgctcgac catcgccaac acatctgcag acgggcggga gaggtcgggt
     1621 gcggcagtca tcggcattcc tcaggtgtcg ttcaggtggc aggcggacat gtgcatcgat
     1681 gcgacctcgc gcagcgccgg gcgttcctcc ttgcagcgcg gcattgcgtg cggacaacga
     1741 ggatggaagt ggcagccgga gggcgggttc aacggcgagg gaatctcccc cttgatcgcc
     1801 acgaactcgg tcttcttcac ttcgagcttc ggcgcggagg ccagcagcgc ggcagtatac
     1861 ggatgattcg gcctggcaaa cacttcggcg gccggagcgg actcgaccac gcgtccgagg
     1921 tacatgatca cgacccgatc cgacaggtgg cgcacaaccc cgaggtcgtg gctgatgaaa
     1981 agataggtga gatcgagttc gtcgcgcagc ttgatgaaca ggttcaggac ctgagcctgg
     2041 atcgagacgt ccaacgccgc caccgcctcg tcgcagacaa ggaattccgg cttcaccgcc
     2101 agcgcccgcg cgatcccgac gcgcgcgcgc tggccgccgg agaactggtg cgggaagcgc
     2161 cgcagcgtcg caggatcgag gccgacccgc tgcagcagac cttccacgta ctcgcgctgt
     2221 tcggcgctgc gcacgatgcc gtgcgcgacc ggcgcctcgc cgacaatgtc ctgcacgcgc
     2281 agtcgtggat tgagcgacgc atacgggtcc tggaaaatca tctggattgc aagctgcttc
     2341 ttgcggcgtt cgcccggatc cagcttgtcg atctcctgcc ccttccacag gcgcgtgcct
     2401 gacgacaggc catgcaggcc cacagccatg cggccgagcg tcgatttgcc gcagcccgac
     2461 tcgccgacca gtccgaccac ctcgcccttg ctgacgacga ggttcacatc gtccactgca
     2521 tggacgacct cttccttctg attagcgcca aagacgttgg cgatcttagc cgcggcatcc
     2581 agcgatttca cgaagcgctt gctgacgtgg cgcatttcca gcaacgcgga ttttccggca
     2641 ctcattgcac tgcctccaac acttctctga caggatgaaa acagcgcagc gcgcgtccgt
     2701 ccacttgccg caattcggga tacgcggtgc acacctcggt ggcgcgatcg cagcgggtgc
     2761 ggaacgcgca gccggtcggg aggtgcagca gcgatggcgt catgccgggg atctggcgca
     2821 gcggctgtcc atgcacgttg cgcgaagggg cggatccgat caacccatag gtgtacgggt
     2881 ggcgcggatg ctcgagcacc tggcgcacgg tgccgctttc cacgatcttc cccgcataca
     2941 tcacgcatac cgaatcggcc agaccggcca ccacagacag gtcgtgcgtg atccagatca
     3001 gcgcggtgcc ggactcgcgg cacagcttct gcatctcgta caggatctgt ccctggatcg
     3061 tcacatccag cgccgtggtg ggttcgtcgc agatgatcag gtcgggtttg ttgagcagcg
     3121 cgatggcgat tgccacgcgc tggcgcatgc cccccgagaa ctggtgcggg taggcgcgca
     3181 gccgttcgtc tggcgacgga atgcccacgc gcgccagcgc atcgcgcgcc atctctcgtg
     3241 cgcgttcgcg gctcacatcc tgatgcgcga acaccgcctc catcatctgg gtgtcgatgc
     3301 gcagcacggg gttcagcgtc atcatcgggt cctggaagat catcgcgatg cggctgccgc
     3361 ggatcttgcg catggcgtcc tggggcaggc cgcgcaggtc ctggcctttc agcaggatct
     3421 tcccgtcaac caccttgccg ggggagtcga tcaagcccat gatcgagtag ccggtcatcg
     3481 acttgcccga cccggactcg ccgaccaggc ccatgatctc gccgcgcttg acggtgaacg
     3541 acacgtcgtc caccgccttc gccacgccgg cgcgcgtgaa gaactgggtt ttcagccctt
     3601 cgacgaccag cgtcggccca tcgtattgcg tcgtcattgc gcgctcctca ttgtgtctgc
     3661 aggcgcggat tcagcacgtc gcgcaactgg tcggcgacca agttgatcgc caccaccgtc
     3721 accagcaagg caatgccggg gaaaaagctg atccagtact tgccggacat cagatactga
     3781 aagccgttgg cgatcaacag gccgagcgac ggttcggtca ccggcaggcc caggccg
//
LOCUS       DATLGF010000240         2317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_5157,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000240 DATLGF010000000
VERSION     DATLGF010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5157"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VM406_07265"
     CDS             <1..769
                     /locus_tag="VM406_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HVL75799.1"
                     /translation="SWMMFIIAVLILRLAPSYRRFGLFGTGFVAPRWVAQRDLLKLGI
                     PMGLSYLIEVTAFTFMALFIARLGASTVGGHQITANFATVLYMLPLAIASATGTLVAQ
                     AIGARDMEGARRLGIDGIRFAAVASVAIGLAVWLARHQIVRAYTPNEVIMAAAVPLFV
                     YIAFYQLFDAIQVVAAFVLRAYKIAIVPTLMYAFALWGIGLSGGYVLGLDPFGISPPA
                     MHGAAGFWFANSASLAVVAVGLLWYLRVVQRDAATRS"
     gene            complement(774..1169)
                     /locus_tag="VM406_07270"
     CDS             complement(774..1169)
                     /locus_tag="VM406_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75800.1"
                     /translation="MSAATAIRDKELTAFVEGRGNTQKVSVIVEANSPILPPARIAAQ
                     TRRAVGLPGPASSRKAVARKGAGSAQVAMADLRRDLEALGLAKNAGENDLACAFVLDV
                     TPAQLRELANLDTVRAIRLNKVYRRIASG"
     gene            complement(1215..>2317)
                     /locus_tag="VM406_07275"
     CDS             complement(1215..>2317)
                     /locus_tag="VM406_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVL75801.1"
                     /translation="FRFQPGWPHFACAAVFPNRRISVPPRMQSAAIPAEIEKRTHYAW
                     GLETSGALTCWGAFGARGAGVKVGVLDTGVEARHPDLVGKVVAFAEFGPNNAMLKAGV
                     THAYDDDGHGTHVCGTIAGGNASGRWIGMAPEAQLIAAKVLKNGVGSDAQILAGMAWA
                     IQQGAHVLNLSLGGWSAGIDVIDTYTNSILQANGVGVPVVTAIGNEGSGTSGSPGTDY
                     FSIGVGATDILDRAAGFSGGRTHVIHASPAFKQKDLPIIFIKPDLSAPGVDVYSCKRG
                     GRWGYESGTSMAAPHVSGAFALLLSGIGANRLPPHDLRRLSNWDRVDVLQSLLLGSAL
                     ELGEKGKNARFGHGRLDIAAAYSNALNLKYLS"
BASE COUNT          363 a          780 c          724 g          450 t
ORIGIN      
        1 gtcctggatg atgttcatca tcgccgtgct gatcctgcgc ctcgctccgt cataccgccg
       61 cttcgggctg ttcggcaccg gctttgtcgc cccgcgctgg gtggcgcagc gcgatctgct
      121 gaaactgggc attccgatgg ggctgagcta tctgattgaa gtgaccgcct tcaccttcat
      181 ggccctgttc atcgcccgcc tcggcgcttc gacagtcggc gggcaccaga tcaccgcgaa
      241 cttcgcgacc gtcctgtaca tgcttccgct ggccatcgcc agcgccaccg gcacgctggt
      301 cgcgcaggcg atcggcgcgc gcgacatgga aggcgcaaga cgcctcggca tcgacggtat
      361 ccgctttgcg gccgtcgcct cggtcgcaat cggtctggcg gtctggctgg cgcggcacca
      421 gatcgtgcgc gcctacacac ccaatgaagt gatcatggcc gccgccgtcc cgctgttcgt
      481 ctacatcgcg ttctatcaac tgttcgatgc gatccaggtc gtcgctgcct tcgtgctgcg
      541 cgcctacaag atcgcaatcg tccccacgct gatgtacgcg ttcgcgctgt ggggcatcgg
      601 gctgagcggc ggctacgtgc tgggcctcga tccgttcggc atcagtccgc cggccatgca
      661 tggcgccgcc ggcttctggt tcgccaacag tgcgagtctt gcggtggtgg cggtcggatt
      721 gctgtggtat ttgcgcgtgg tgcagcgtga cgcggcgaca cgcagctgaa aggctagccg
      781 gaagcgatcc gccggtagac cttgttgagg cggattgcgc gaaccgtgtc caggttggcc
      841 agctcgcgca attgggcggg ggtgacatca agcacgaagg cgcacgccag atcgttctct
      901 ccggcattct tcgcgagacc aagcgcttcc agatcgcggc gcaggtcggc catcgcgacc
      961 tgagcacttc ccgcgccctt gcgggcgacg gcctttcgtg acgaagccgg gccgggcagc
     1021 cccactgcgc ggcgggtctg ggcggcgata cgcgcgggcg gcaggatagg gctgttggct
     1081 tctacaatca ccgagacttt ctgcgtgttg ccacgccctt ccacgaaggc agtcaactcc
     1141 ttgtcccgga ttgcagtcgc ggcgctcata cccgtttctc ctttcggcta ggataacttt
     1201 tccacctgct ttgatcaaga aagatacttt agattcaacg cgttactgta agcggccgca
     1261 atatccagtc gcccgtgtcc aaagcgggcg ttcttgcctt tttcccccag ctccagtgcg
     1321 ctgccgagca gcagtgactg caggacgtcg actcggtccc aattgctcag tcgcctgaga
     1381 tcgtgcggcg gcagcctgtt cgcgccgatg ccgctcagca gaagcgcgaa tgcgccgctg
     1441 acgtggggag cggccatcga ggtcccgctc tcgtaacccc agcgcccgcc ccgcttgcag
     1501 gagtagacat cgacacccgg cgccgagaga tcaggcttga tgaaaatgat gggcaggtct
     1561 ttctgcttga aagccggcga ggcgtggatc acgtgggtgc ggccgccgct gaaacccgcc
     1621 gcccgatcca ggatatcggt ggcgccgaca ccgattgaga aatagtcggt gccgggcgaa
     1681 ccgctggtgc cgctgccttc gttgccgatc gccgtcacca ccggcacccc gacgccgttt
     1741 gcctgcagga tgctgttcgt ataggtatcg atcacgtcga tgccggcgct ccagccgccc
     1801 agggacaggt tgagcacatg ggcgccctgc tggatcgccc atgccattcc ggcgaggatc
     1861 tgcgcatcgc tgcccacgcc gttcttgagc accttggcgg caatcagctg tgcttccggc
     1921 gccatgccga tccagcggcc gctcgcattg ccgccggcaa tggtgccgca cacatgggtg
     1981 ccgtggccgt catcgtcgta tgcatgcgtg acgccggcct tcagcatcgc attgttcggg
     2041 ccgaactcgg caaacgcaac gaccttcccg accaggtccg gatgcctggc ctctacgcca
     2101 gtgtcgagca ccccgacctt cacgcccgcc ccgcgtgcgc cgaacgcgcc ccagcaggtc
     2161 agtgcgcccg aggtctccag gccccacgcg tagtgcgtgc gcttttcgat ttcagccggg
     2221 atggccgcac tttgcatgcg cggcggcacc gatatccgcc ggttcggaaa caccgccgcg
     2281 catgcaaagt gcggccatcc cggctgaaat cgaaaag
//
LOCUS       DATLGF010000241         6093 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_42485,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000241 DATLGF010000000
VERSION     DATLGF010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42485"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..941)
                     /gene="rsmI"
                     /locus_tag="VM406_07280"
     CDS             complement(21..941)
                     /gene="rsmI"
                     /locus_tag="VM406_07280"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HVL75802.1"
                     /translation="MSPTSSAAPDLHAIMQEVARQTYPTPALYVVSTPIGNVGDLTLR
                     AVNTLALADAVACEDTRNTAALLNRYGLHKELIAAHEHNEREAAERLIARLQSGQRIA
                     LVSDAGTPGISDPGARIVDAVREAGLRVIPLPGPSAAIAALSASGLVNERFHFIGFLP
                     ARTAQREASLGALARETSSLVFYEAPHRIEETVASLVAVFGPARRIVFARELTKLFEE
                     VHRCQLGEAGAWLAADANRGKGEYVIVIEGAQAATDQDQAEAERVLRILLAECPVKQA
                     VALAAKLTGQKKNALYERALQLKADGESGV"
     gene            1080..2270
                     /locus_tag="VM406_07285"
     CDS             1080..2270
                     /locus_tag="VM406_07285"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HVL75803.1"
                     /translation="MRPLGEADAQVRFPQPEALHAQPAAQAVPPATAAAQPVPVPVPV
                     PVPVPATPIGPRARIALMLPLRSSSLGGAAEAVRAGFFAAYQVERQGVEITLVETGDS
                     PQDVLAAYRSASATHDIVVGPLSRSGVTAVARSGEVMRPTVALNAPDAPEDAAVRPPP
                     LMLVMGLSVEDEARQVADWARRDHASGTAYVVHTATAWQRRAARAFATQWAASGMAVR
                     TVELEVADGFLNAAAMLQLRTRLENERAPFVFAALDAGQARQLRESVGRQYPLFGTSQ
                     LNPIALQDRALVEASPAMDGALLLDIPWQLQDDHPAVMAYPRLPGDAEQRRSADMERL
                     YALGIDAYRVARQLAAQHTNFELDGVTGRLVVRFDRFGPHFERTTQQAVYRGGSVAPA
                     TVAR"
     gene            2280..2648
                     /locus_tag="VM406_07290"
     CDS             2280..2648
                     /locus_tag="VM406_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HVL75804.1"
                     /translation="MKWLNPLARSPKQLDGAAAEERARAFLQAKGLVPVQANFRCKGG
                     EIDLIMRDGEVLVFVEVRKRSGTGFGGAAASVTARKQARLILAAQLYLQRYRFPPACR
                     FDVIAIDAGEIDWLRNAFTA"
     gene            2672..3268
                     /locus_tag="VM406_07295"
     CDS             2672..3268
                     /locus_tag="VM406_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HVL75805.1"
                     /translation="MNKQRIEAHFQESAELKMKAARELAEPISQAVELMFSALSNGNK
                     ILACGNGGSAGDCQHFAAELVGRFERERLPLPAMALTTDSSILTAVSNDYSFQEIFSK
                     QVQAFGQSGDILLAISTSGKSPNVLAAVDAALERDMRVVALTGKGGGAIAQRLTDADV
                     HICVPHDRTARIQEVHLLVIHCLCDGIDVALFGEDGHD"
     gene            3261..3920
                     /locus_tag="VM406_07300"
     CDS             3261..3920
                     /locus_tag="VM406_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HVL75806.1"
                     /translation="MIKTGAKFRQRAVRPVATLLIAASAALALQGCAGVVLGTAVMTS
                     LAATDRRTLGAQTEDKAIFLKAQPAARRAAGESSNVNVTSFNRQVLLTGEVRDEATKA
                     AVEREVARVDGVLSIVNDIAVTMPSSLASRSNDTLITGKVKASFVDAKDIFANSIKVV
                     TERGNVYLMGRVTEREGQRAAEVASRVGGVQKVVKVFDYITEAELQGLAQTPGNTQSG
                     R"
     gene            3998..4588
                     /locus_tag="VM406_07305"
     CDS             3998..4588
                     /locus_tag="VM406_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HVL75807.1"
                     /translation="MTALVILAAILLLVLFWGVGAYNRLINLRNQFKNAFAQIDVQLK
                     RRYDLIPNLVETAKAYMKHERETLEAVIAARNQAVAASTRAAGDPTDEAAIRSVAAAE
                     GALGSALGRLFALSEAYPDLKANQNMMQLTEELTSTENRIAFARQAYNDGVMQYNIAR
                     EQFPSSIIAGMFSFRHAEQLQATETAEERKAVKVSF"
     gene            4704..>6093
                     /locus_tag="VM406_07310"
     CDS             4704..>6093
                     /locus_tag="VM406_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HVL75808.1"
                     /translation="MNFFEQQEHARRQTRKLVILFILAVIAIVVAVNVALAFLWSWTA
                     NGQVLGVQDYPRGFFATNTFIVLAMIGGGTLIETFRLREGGDAVARMAGGRLVSPATT
                     DAQERRLLNVVEEMALASGVACPRVYLLDQEQSINAFAAGYNQDHAVIGVTRGTMERL
                     TRDELQGVIAHEFSHILNGDMRMNIRLIGLLFGIQMLAAFGQHLMSFGSRAMVVRSRD
                     DRGGQVRAVLLLTGVALFVIGYIGVIFGRLIKSAVSRQREFLADASAVQFTRNPDGIG
                     GALRKIGGLSRRGEPGSQINHPNAEQLSHLFLGAPRQSMISGWLATHPPIEERLRRIF
                     GRSMPMLEAPPIAPAPVAARTLPDIPYTASGFAGDQAQDSWMPAGTQVAANGLPPAVD
                     SSVHDPFGAAALVYAMLLNDGGEGRARQLALLEQQASVHAKQVTVLAGEVAALPRSAR
                     LPLLDRAMPALLM"
BASE COUNT          997 a         2021 c         2052 g         1023 t
ORIGIN      
        1 cagcggtagt tggttcagcc tcagacgccc gactcgccgt ccgccttcag ctgcaatgcc
       61 cgctcgtaga gcgcgttctt tttctggccg gtcagtttcg ccgcgagggc gacagcctgc
      121 ttcaccgggc attccgcaag caggatgcgc agcacgcgct cggcttccgc ctggtcctgg
      181 tcggtggcgg cttgcgcgcc ttcgatcacg atcacatatt cgcccttgcc gcggttggca
      241 tccgccgcca gccatgctcc cgcctcgccc aactggcagc gatgcacttc ctcgaacagc
      301 ttggtgagct cacgggcgaa cacgattctg cgtgcggggc cgaacaccgc cacgagactc
      361 gccaccgttt cctcgatgcg gtgcggggcc tcgtagaaca ccagcgacga ggtttcgcgc
      421 gccaatgcgc caagggacgc ttcccgctgc gcggtgcgtg ccggaaggaa gccgatgaaa
      481 tggaagcgct cgttgaccag accgctggcc gacagcgcgg caatggcggc tgacggcccc
      541 ggtagcggga tcacacgcag gcctgcctca cgcaccgcat cgacgatgcg cgcgcccgga
      601 tcggagatgc cgggcgtgcc ggcgtcggag accagagcga tgcgctggcc cgactgcagg
      661 cgcgcaatca ggcgttcggc cgcctcgcgc tcgttgtgct cgtgcgcggc gatcaactcc
      721 ttgtgcagcc catagcgatt cagcaacgcc gcagtgttgc gcgtgtcttc gcaggccacc
      781 gcatctgcaa gcgcgagcgt gttgacagca cgcaatgtca ggtcgccaac gtttccgatg
      841 ggagtgctca ctacgtataa tgccggcgtt ggataagtct gccgggcaac ttcctgcatg
      901 atggcgtgca ggtctggggc cgcggaggat gtcgggctca tcgggagaat gtgatgttgt
      961 ttcaaaggac gaaggccctg atgctggcca tggtgtgcgg attgtgcctg tcgcttcata
     1021 cgggcgcaaa tgcgcagggg gcggagacgt tctcggtaca catgccaaac ggcgtgtcga
     1081 tgcgtccgct tggtgaggcg gatgcgcagg tccggttccc gcaaccggag gcactgcacg
     1141 cgcagcccgc cgcgcaggcg gtcccgccgg ccactgctgc tgcccaacct gtccctgtcc
     1201 ctgtccctgt ccctgtccct gttccggcaa cgccgatcgg cccacgtgcg cgcatcgcgc
     1261 tgatgcttcc gctcaggtcg agcagccttg gcggcgcagc cgaggctgtg cgcgccggtt
     1321 tcttcgccgc ttaccaggta gagcgccaag gcgtggagat cacgctcgtc gagaccggcg
     1381 attctcccca ggacgtactc gccgcctacc gcagcgcaag tgcaacgcat gacatcgtgg
     1441 tcgggccgct gtcgcgttcc ggcgtcaccg ctgttgcccg cagcggggag gtgatgcgtc
     1501 ccaccgtggc gctgaacgcg ccggatgcgc cggaagacgc ggccgtacgg ccgccgccgc
     1561 tgatgctggt gatgggcctg tccgtcgagg acgaggcgcg ccaggtggcc gattgggcgc
     1621 ggcgcgatca cgccagcggc accgcatacg tggtgcacac cgccaccgcc tggcagcgcc
     1681 gtgccgcgcg ggccttcgcc acccagtggg cagccagcgg catggctgtg cgcaccgtcg
     1741 agctggaggt ggccgacggc ttcctgaacg ccgcagcgat gctgcagctg cgcacgcgcc
     1801 tggaaaacga gcgcgcaccc ttcgtgtttg ccgcgcttga tgccggccag gcacgccagc
     1861 tgcgcgagag cgtggggcgt cagtacccgc tgttcggcac ctcgcagttg aatccgatcg
     1921 cgctgcagga ccgcgcactg gtcgaagcgt cgccagcgat ggacggcgcc ctcctgctcg
     1981 acattccgtg gcagctgcag gacgatcatc cggcggtgat ggcatacccg cgcctgccgg
     2041 gcgatgccga gcagcgccgc agcgcagata tggagcgcct gtatgcgctg ggcattgacg
     2101 cctaccgggt cgcgcgccaa cttgcggccc agcacaccaa cttcgaactc gacggcgtga
     2161 ccgggcgcct ggtcgtgcgc ttcgaccgtt tcgggccgca cttcgaacgt acgacgcagc
     2221 aggcggtgta ccgcggcgga agcgtggcgc cggcgacggt tgcgcgctga tccgtcccga
     2281 tgaaatggct caatccgctc gcgcgcagcc cgaagcagct tgacggcgcg gcggcggaag
     2341 agcgggcgcg cgcctttctg caggcgaagg ggctggtgcc ggtccaggcg aactttcgct
     2401 gcaagggcgg cgagatcgac ctgatcatgc gcgacggcga agtgctcgtg ttcgtcgaag
     2461 tgcgcaagcg cagcggtaca ggcttcggcg gcgccgcggc cagcgtcacc gcgcgcaagc
     2521 aggcccgcct cattcttgcc gcccagctct acctgcagcg ttatcgattt cctcccgcct
     2581 gccggttcga cgtgatcgca atcgatgccg gcgagatcga ctggctgcgc aatgcgttca
     2641 ccgcgtagcc gcccacctat aatccccgac catgaacaag caacgaatcg aggcgcactt
     2701 ccaggaaagc gccgaactga agatgaaggc cgcacgcgaa ttggccgaac cgatatcgca
     2761 ggccgtggaa cttatgttct cggccttgtc taacggcaac aagatccttg cttgcgggaa
     2821 tggcggatcg gccggcgact gtcagcattt cgccgccgaa ctggtcggcc gtttcgaacg
     2881 cgagcgcctg ccgctgcctg ccatggcgct taccacggac agttccattt tgaccgcggt
     2941 atcgaatgac tacagcttcc aggagatctt cagcaagcag gtgcaggcat tcgggcagtc
     3001 cggagacatc ctgctcgcca tttccacgtc cggcaaatcg ccgaacgtgc tggcggcggt
     3061 ggatgcggcg ctcgagcgcg acatgcgcgt ggtcgcgctg accggcaagg gcggcggcgc
     3121 gattgcgcag cggctcaccg acgcggacgt gcacatctgt gtgccgcacg accgcacggc
     3181 gcgcatccag gaagtgcacc tgctggtgat tcactgttta tgcgacggca tcgacgtcgc
     3241 cttgtttggg gaggatggtc atgattaaaa cgggagcgaa attccgtcaa cgcgcggtgc
     3301 ggccggtggc cacgctgctg atcgcggcca gcgccgcact cgcgctgcaa ggctgcgccg
     3361 gcgtcgtgct gggcacggcg gtgatgacgt ccctggcggc gacggatcgc cgcacactgg
     3421 gggcgcagac cgaggacaag gcgatcttcc tgaaggcgca gccggccgcg cgccgggcgg
     3481 ccggcgagtc gtcgaacgta aacgtcacca gcttcaaccg ccaggtgctg ctgaccggcg
     3541 aggtgcgcga cgaggcaacc aaggccgcgg tggagcgcga agtggcgcgc gtcgacggcg
     3601 tgctttcgat cgtcaacgat atcgccgtca cgatgccttc cagcctggcc tcgcgctcca
     3661 acgacacgct gatcaccggc aaggtgaagg cctcgttcgt cgacgccaag gatatcttcg
     3721 ccaattcgat caaggtcgtg accgagcgcg gcaatgtcta cctgatgggc cgcgtgaccg
     3781 aacgcgaagg ccagcgcgcc gccgaggtcg ccagccgcgt cggcggggtg cagaaggtcg
     3841 tcaaggtatt cgactacatc accgaggccg aactgcaggg gctggcccag acccccggga
     3901 acacgcagtc ggggcggtga ggaatcggca agtgccgatg gtatagttgg ccgccggtcg
     3961 gaaacgacct gcggccattt tcattctagg agacagcatg accgcactcg tgattctcgc
     4021 agccatcctg ctgctggtcc tgttctgggg ggtgggcgcg tacaaccgcc tgatcaacct
     4081 gcgcaaccag ttcaagaacg cattcgcgca gatcgacgtg cagttgaagc gccgctacga
     4141 tttgatcccc aatctcgtcg aaacggcgaa ggcctacatg aagcacgagc gtgaaacgct
     4201 cgaggcggtg atcgccgcgc gcaatcaggc cgtcgcggcc agcacgcgcg cggccggcga
     4261 cccgaccgac gaggcggcga tccggtccgt ggccgcggcc gagggcgcgc tgggctcggc
     4321 gctcgggcgc ctgttcgcac tgtcggaagc gtatccggac ctgaaggcca accagaacat
     4381 gatgcagctg accgaggagc tgacctcgac ggaaaatcgc atcgccttcg cgcgccaggc
     4441 ttacaacgac ggcgtgatgc agtacaacat cgcgcgcgag cagttcccga gttcgatcat
     4501 cgccggcatg ttctcgttca ggcacgccga gcagctgcag gcaaccgaga ccgccgaaga
     4561 gcgcaaggcg gtgaaggtct ccttctgatc ctgcgcgcgg tgcagccgtc cgcttcgttc
     4621 tcattccaat ggaggctggc ggggatggga ggacggcgcc agcgccagct ctttcccccc
     4681 agggaactgc tgaccaggtc cgtatgaact tcttcgaaca gcaagaacac gcccgccgcc
     4741 agacccgcaa gctggtcatc ctgttcatcc tcgcggtgat cgcgatcgtc gtcgcggtta
     4801 acgtcgcgct cgcgttcctg tggagctgga ccgcgaacgg ccaggtgctc ggcgtccagg
     4861 actatccgcg cggttttttc gcgaccaata ccttcatcgt gctggcgatg ataggcggcg
     4921 gcacgctgat cgagaccttc cgtctgcgcg agggcgggga cgcggtcgcg cgcatggccg
     4981 gcggccgtct cgtttcaccc gccaccaccg atgcccagga gcgccggctg ctgaacgtgg
     5041 tcgaggagat ggcgctggcg tccggagttg cctgcccgcg cgtctatctg ctcgaccagg
     5101 agcagtcgat caatgcgttc gcggccggct acaaccagga ccatgcggtg atcggcgtca
     5161 cgcgcggaac catggagcgg ctcacgcgcg acgaactgca aggcgtgatc gcgcacgagt
     5221 tcagccatat cctcaatggc gacatgcgca tgaacatccg cctgatcggc ctgttgttcg
     5281 gcatccagat gctggccgcg ttcggacagc atctgatgag cttcggctcg cgcgcgatgg
     5341 tcgtgcgcag ccgcgacgac cgcggcgggc aggtgagggc ggtcttgctg ctcaccggcg
     5401 tggcgctgtt cgtgatcggc tacatcggtg tgatcttcgg ccgtctgatc aaatccgcgg
     5461 tctcgcgcca gcgcgaattc ctcgccgatg ccagtgcggt gcagttcacc cgcaatcctg
     5521 acggcatcgg cggcgcgctg cgcaagatcg gcgggttgtc gcgtcgcggc gagcccggct
     5581 cccagatcaa tcatccgaac gccgagcagc tctcccattt gttcctgggc gcgccgcgcc
     5641 agagcatgat ttccggctgg ctggccacgc atccgccgat cgaggagcgc ctgcgccgca
     5701 tcttcgggcg cagcatgccg atgctcgagg cgccgccgat tgcgccggcg ccggtcgccg
     5761 cgcgcacgct acccgatatc ccgtacaccg cctccggctt tgccggcgac caggcgcagg
     5821 acagctggat gccggcgggc acgcaggtgg ccgcgaacgg cctgccgccc gccgtggaca
     5881 gctccgtgca cgatccgttc ggcgcggctg cgctggtcta cgccatgctc ctcaacgacg
     5941 gcggcgaggg ccgcgccaga cagttggcat tgctggagca gcaggcgtcg gtgcatgcga
     6001 agcaagtcac ggtgttggct ggtgaagtgg cggcgctgcc gcgcagcgcg cgtctgccct
     6061 tgctcgaccg cgcgatgccg gcgctgttga tgc
//
LOCUS       DATLGF010000242         4659 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_26560,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000242 DATLGF010000000
VERSION     DATLGF010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26560"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /gene="dinB"
                     /locus_tag="VM406_07315"
     CDS             complement(<1..1010)
                     /gene="dinB"
                     /locus_tag="VM406_07315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HVL75809.1"
                     /translation="MHAPERKIIHCDCDCFYAAVEMRDDPSLRGRPLAVGGRADRRGV
                     IATCNYEARRYGVRSAMSTAQALQRCPDLIVLPPAMDKYRIASRQILAIYRDYTELVE
                     PLSLDEAYLDVTASPQWRNSATLIAQDIRARIRETVGITASAGVAPNKFIAKIASDWN
                     KPDGQFVVRPQDVDAFVAALPVDKLFGVGKVTAQKLKRLGAHTCADLRAWDLAELQRH
                     FGKFGSALYNLSRGIDRREVKPDRERKSVSVEETYARDLPDLAACRRELPALIERLDQ
                     RMRRAQAGDTVRKLFVKIRFDDFRRTTAECVSSTVEFAACEDLLQTAWRRGARPVRLL
                     GVG"
     gene            complement(1074..1469)
                     /locus_tag="VM406_07320"
     CDS             complement(1074..1469)
                     /locus_tag="VM406_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75810.1"
                     /translation="MKRTFHGLAGAALLACAPLAVAQGAIDPTFQPTQPAPEAAAPAT
                     GGPATFATDASGASAVRQGSIAGSAHGTDRAAAARSAAEMSNDPFVQRREARAQARAE
                     YRQRVRDAREQLREDRRAADALLHPQSGY"
     gene            1658..2107
                     /locus_tag="VM406_07325"
     CDS             1658..2107
                     /locus_tag="VM406_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75811.1"
                     /translation="MASSTLDPDNMPERDRRLGSGHGTRALGPSDTSDSGSDVQDSMR
                     WTNEADLGLDRGTLEDSDSERADRTAGPDIGDAWLDSDTDARGTGEVATAGRDPAVEM
                     GGDIGTDHIESIAGEDEMAASDEDADEGVPSLSREDRERAQQRPSRH"
     gene            2120..3040
                     /locus_tag="VM406_07330"
     CDS             2120..3040
                     /locus_tag="VM406_07330"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVL75812.1"
                     /translation="MASDDDLLDCIIVGAGAAGLTAAVYLARFRRRIALLDGAGSRLL
                     LIPESHNYPGYAHGIHGPDVLARLREQALQYDVQVQNTTIDSLERDAHGNFVARAEHR
                     SWRARTALLATGVTDVAPPFAEVSTALATGRLRYCPICDGYEAIGRSAAVLGRGNSGV
                     EEAIFVRHFADKVYVCALDDPLQLDDGQRERLARARVDVVAPPVTALGLSNGPHERMW
                     LALDDGGELDVDIVYAALGTLVNSGLGQLLGAQCADNDELLVDAHQQTSVDGLYAAGD
                     VVIGLNQIAVAMGHAAIAATAIHNRLRTRQ"
     gene            complement(3034..3450)
                     /locus_tag="VM406_07335"
     CDS             complement(3034..3450)
                     /locus_tag="VM406_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75813.1"
                     /translation="MKAGQRTEWPVLWTALGALAMLMIGFGAAWAWLQHAERQRVTSS
                     FTMLKPLAVSGSGHSVGATLAVKTSEADKRWAGQNRAGLEAALQQALLGADPARALAP
                     NGLVELQHSLRQVANGSLSTDKIQEIVITDFLVTVY"
     gene            complement(3447..3767)
                     /locus_tag="VM406_07340"
     CDS             complement(3447..3767)
                     /locus_tag="VM406_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2288 domain-containing protein"
                     /protein_id="HVL75814.1"
                     /translation="MSDPDSDDALLRAKLNGETSRMPWHELQRFFAGGSMIVVDDTLD
                     LVEVALRIARDDKAAVAAWLEQGKIARASDEHARGWTEADASLWAVVVKPWVLVQREK
                     RHAA"
     gene            complement(3767..3976)
                     /locus_tag="VM406_07345"
     CDS             complement(3767..3976)
                     /locus_tag="VM406_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HVL75815.1"
                     /translation="MSTSEERLVDLEIRIARQDDLLDVLNQTVYRQQKKIDELEALCT
                     ALARHVKDMRDAAAAAPANDKPPHY"
     gene            complement(4006..>4659)
                     /locus_tag="VM406_07350"
     CDS             complement(4006..>4659)
                     /locus_tag="VM406_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL75816.1"
                     /translation="VLLTQSDSFMGPALQAVFAECGAEVIADRRALDAPDAAAQAVAA
                     AGDIDVLLLNLAVPAPTTPAAQVDDTEWRDVFAHMVDPLPRLVRAVLPKMIARRRGKI
                     LLIGSAAALRGMKRTSSYSAARGAQLAYIQALGVEAAPHNVQVNAIAQNFVENPTYFP
                     PEVQANPAFQERLKREVPLGRLVSQREDAQFAAYLCSDAADCFVGQVFPVCGGWVAR"
BASE COUNT          769 a         1563 c         1566 g          761 t
ORIGIN      
        1 ccgacgccta gcaggcgcac cggacgcgcg ccccgccgcc acgcggtctg cagcaggtct
       61 tcgcacgcag cgaactccac cgtgctgctc acgcattcgg cggtggtacg gcggaaatcg
      121 tcgaagcgga tcttgacgaa cagcttgcgc accgtatcgc ccgcctgggc acggcgcatg
      181 cgttggtcca gccgttcgat cagcgccggc aattcgcgcc ggcacgcggc aaggtccggc
      241 agatcgcgcg cataggtctc ttccacgctg acggacttgc gttcgcgatc aggcttcact
      301 tcacggcggt cgatgccgcg cgacaggttg tagagcgcgc tgccgaactt gccgaaatga
      361 cgctgcagct cggccaggtc ccatgcgcgc aggtcggcgc aggtatgggc cccgagccgc
      421 ttgagctttt gcgcggtcac cttgcccacg ccgaacaact tgtcgaccgg cagcgcggcg
      481 acgaatgcgt cgacgtcctg cggacgcacg acgaactggc cgtccggctt gttccagtcg
      541 ctggcaattt ttgcaatgaa cttgttcggc gcgacgccgg ccgaggccgt gatgccaacg
      601 gtctcgcgaa tgcgcgcgcg gatgtcctgc gcgatcagcg ttgcgctgtt gcgccactgc
      661 ggggacgcgg tgacgtccag atacgcctcg tcgagcgaga gcggttcgac cagctccgta
      721 tagtcgcgat agatcgcaag gatctggcgc gacgcgatgc ggtacttgtc cattgccggc
      781 ggcagcacga tcaaatccgg acagcgctgc agcgcctgcg cggtggacat cgcggagcgc
      841 acgccgtagc ggcgcgcctc atagttgcag gtggcgatca cgccgcgacg gtcggcgcgg
      901 ccgcccacgg cgagcggccg gccgcgcagc gagggatcgt cgcgcatctc gactgctgca
      961 tagaagcagt cgcaatcgca gtggatgatt tttcgttcgg gcgcgtgcac gtgcgcgggg
     1021 atggaggaag gacggctgtc attctaccgg ccctgccccg cgctttcaga cggtcaatac
     1081 ccggattgcg gatgcagcaa tgcatccgcc gcgcgccggt cctcgcgcag ctgctcgcgc
     1141 gcatcgcgca cccgctggcg gtactctgcc cgggcctgcg cgcgcgcttc gcgccgctgc
     1201 acgaagggat cattgctcat ctcggccgcg ctgcgtgcag cggccgccct gtcggtcccg
     1261 tgcgcactgc cggcgatgct gccctgacgc acggccgaag caccggatgc atcagttgca
     1321 aacgttgctg gaccgcctgt cgccggcgcg gctgcttcgg gcgcaggctg ggtaggctga
     1381 aacgtcgggt cgattgcgcc ctgggcaacg gcaagcggtg cgcacgcgag caaggccgca
     1441 ccggccaggc catgaaatgt tcttttcatc ttgcactcct ttcagattgc ggtcggaccg
     1501 cgacacgcat tgcattcatg cgctttgccg gcggtcatct ctgtcactga gtgacccagc
     1561 gtcaaaaatg aagttccggc acgaccattc acggcatggt tattgcaggg ttcaccgact
     1621 acgcgttttg ggcgcgaaaa ccttgattgg agcgactatg gcaagcagca cactggaccc
     1681 ggacaacatg ccggaaaggg accgccgcct cggcagcggt catggcacgc gcgcactcgg
     1741 accgagcgac acctcggaca gcggcagtga cgtgcaggac agcatgcgct ggaccaacga
     1801 agccgacctc ggcctggacc gtggcacgct cgaggattcc gacagcgagc gcgcggaccg
     1861 cactgccggt cccgacatcg gcgatgcatg gctggactcg gataccgatg cgcgcggcac
     1921 cggcgaagtc gccacggccg gacgcgatcc ggcagtggag atgggcggcg acatcggcac
     1981 cgaccacata gaaagcattg ccggcgagga tgaaatggcg gccagtgacg aggatgcgga
     2041 cgaaggcgtc ccctcgttgt cacgcgagga ccgcgagcgc gcgcagcagc gcccgagccg
     2101 tcattgagag gggagcggca tggcaagcga cgacgatctg cttgactgca tcatcgtcgg
     2161 cgccggcgcg gccgggttga cggccgccgt gtacctcgcc cgcttccgcc gccgcattgc
     2221 cctgctcgac ggcgccggca gccggctgct gctgattccc gaatcgcata actatcccgg
     2281 ttatgcgcac ggcattcacg gccccgatgt gctggcgcgc ctgcgcgagc aggcgctgca
     2341 gtacgacgtg caggtgcaga acacgaccat cgacagcctg gagcgcgatg cgcacggcaa
     2401 cttcgtcgcg cgcgcagaac accgcagctg gcgcgcgcgc accgcgttgc ttgctaccgg
     2461 cgtgaccgac gtcgcgccgc cgtttgccga agtctcaaca gcgctcgcca ccggccggct
     2521 gcgctactgc ccgatctgcg acggctacga ggcgatcggc cgcagcgcgg cggtgctggg
     2581 acggggcaac agcggcgtgg aggaagcgat cttcgtgcgc cacttcgccg acaaggtcta
     2641 cgtctgcgcg ctggacgatc cactgcagct cgacgacggg cagcgcgagc ggctcgcgcg
     2701 cgcgcgcgtg gacgtcgtcg cgccgccggt caccgcactc gggctttcaa atggtcccca
     2761 tgagcgcatg tggctggcgc tggatgatgg cggcgaactc gatgtcgata tcgtctacgc
     2821 ggcgctgggc acgctggtga actcaggcct gggacaactg ctgggcgcgc aatgcgcgga
     2881 caacgatgaa ctgctcgtcg acgcgcatca gcagaccagc gtcgacggcc tgtatgcggc
     2941 cggggatgtg gtgatcgggt tgaaccagat cgcggtggcg atgggacacg cggcgattgc
     3001 ggcgacggcg atccacaacc gcctgcgcac gcgtcagtaa acggtaacca ggaagtcggt
     3061 gatcacgatt tcctggatct tgtcggtgga caggctgccg tttgcaacct ggcgcaggct
     3121 gtgctgcagc tcgacaaggc cgttcggcgc cagtgcgcgc gccggatccg cgccgagcag
     3181 cgcctgttgc agtgccgctt ccaggccggc acggttttgg cctgcccagc gcttgtccgc
     3241 ctcgctggtc ttgacggcga gcgtggcgcc gaccgaatgt ccgctgccgc tgaccgccag
     3301 cggcttgagc atcgtgaagc tgctggtcac gcgctggcgt tccgcgtgct gcagccacgc
     3361 ccaggccgcg ccgaagccga tcattagcat cgcaagcgca cccagcgcgg tccacaggac
     3421 cggccattcg gttcgctgtc cggccttcat gcagcgtgcc gtttttcgcg ctgtaccagc
     3481 acccagggct tgaccaccac cgcccacagg ctggcatcgg cttcggtcca gccacgcgcg
     3541 tgctcgtcgg acgcgcgggc gatcttgccc tgctccagcc aggccgcgac agccgccttg
     3601 tcatcgcggg caatacgcaa cgcgacctcg accagatcca gcgtatcgtc gacgacgatc
     3661 atgctgccgc cggcaaagaa acgctgcaat tcgtgccacg gcatgcgcga ggtttcgcca
     3721 ttgagcttgg cgcgcagcag cgcatcgtcg gaatcgggat cactcatcag taatgcgggg
     3781 gtttgtcgtt ggcgggcgcg gccgctgccg cgtcgcgcat gtccttcaca tgacgcgcga
     3841 gcgcggtgca cagcgcttcc agttcgtcga tcttcttctg ctggcgatag acggtctggt
     3901 tcagcacatc gagcagatcg tcctggcgcg cgatgcggat ctccaggtcg accagacgtt
     3961 cttcggaagt ggacatgtca acggaatgga taagagcccg ccattctaac gcgcgaccca
     4021 gcccccgcat acgggaaaca cctgcccgac gaagcagtcg gccgcgtcgc tgcacagata
     4081 ggcggcgaac tgcgcgtcct cgcgctggct gaccaggcgg ccgagcggca cttcgcgctt
     4141 caggcgttcc tggaacgctg gattggcctg cacctcgggc gggaaatagg tcgggttctc
     4201 gacgaaattc tgcgcaatcg cgttgacctg cacattgtgc ggcgccgcct ccacgcccaa
     4261 tgcctgaatg taggcgagct gggcgccgcg cgcggcgctg tagctcgacg tgcgcttcat
     4321 gccgcgcagc gcggcggcac tgccgatcag caggatcttg ccgcgtcgcc gcgcgatcat
     4381 cttcggcagc accgcgcgca ccagccgcgg cagcggatcg accatgtgcg caaacacgtc
     4441 acgccattcc gtatcgtcga cctgcgcggc aggcgtggtc ggggccggca ccgcaaggtt
     4501 gagcagcagc acatcgatgt cgccggctgc cgccaccgcc tgcgcggcgg cgtcgggcgc
     4561 atccagcgcg cgccggtcgg caatcacctc ggcgccgcat tcggcgaaga cggcctgcaa
     4621 tgcagggccc atgaagctgt cggactgggt cagcaagac
//
LOCUS       DATLGF010000243         4624 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_69564,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000243 DATLGF010000000
VERSION     DATLGF010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69564"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..931)
                     /locus_tag="VM406_07355"
     CDS             complement(131..931)
                     /locus_tag="VM406_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVL75817.1"
                     /translation="MATRDESIDIHVDSARIAGTLVRPAPRLPGVLFVHGWGGSQEQY
                     LARAHEIAALGCFCLTFDLRGHAGTHGQHETVTRESNLRDVLAAYDVLASHPQTDPDA
                     IAVVGSSYGGYMAAMLTALRPVRWLALRAPALYPDDGWHLPKRQLHKGDDLEIYRRSV
                     VPPGDNRALRACSRFAGDVLVVESECDKVIPHPVITSYVEACSRARSLTYRVISRADH
                     GLSDDASQRAYTALLVNWMREMVRGDLLEPVAAEPPQHAIDPVQSRAA"
     gene            complement(915..2045)
                     /locus_tag="VM406_07360"
     CDS             complement(915..2045)
                     /locus_tag="VM406_07360"
                     /inference="COORDINATES: protein motif:HMM:NF022821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3182 family protein"
                     /protein_id="HVL75818.1"
                     /translation="MTSLNRRRDGAREVVYLARPGVNPTGHECATQRALAAALAELKG
                     WPFGGDYDPERHCAARAYFVPGDTLEAGAAARLGVHGEHDLFGGVVPFPFVATKTISH
                     PLVHLGAQAPLGWSHGFGQRVRSVVLPGYSAFVLDDARAAGRRLLECGSVRIKLGGGV
                     GGVGQSVARTAVELEDVLASIEAGPGFGPGVVLETNLEQVDTLSAGRTRIDDLVMSYY
                     GRQRLTTDNIGRHVYGGSELFVVRGEFDVLRARVLPDEVRIALEQAQHYHAAALESFP
                     GLCASRCNYDIAQGRDDEGNWLSGVLEQSWRIGGASGAELLAFAAFLGDPALQAVRAT
                     TIEVYGEAPPVPEDAQVLFSGVDPKVGRITKYARIEGYGDAG"
     gene            complement(2147..3184)
                     /locus_tag="VM406_07365"
     CDS             complement(2147..3184)
                     /locus_tag="VM406_07365"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HVL75819.1"
                     /translation="MNTLHRKLAAGILVPWLAFGTAAVAQQQQQAPTAGAPPLTAEQL
                     ALLDVRASELIGKDVTNPQGQDLGRIEDLIIDVANSQVRYAIVSHGGVLGIGNQMAAF
                     PPVMFRPGREEGEVMLNVSQQQLEQAPRFDAREWPDWNEESYRGQVDRFFWQDDEVIR
                     KPSGSRLARASDVIGKRVNDGGGRHAGSIEDIVVNLGNGHLRYAVLDYSATRFGIGGG
                     HLVPLPIAAFRFPTRPDIPIFLVLDRERVEQARAFDRDNWPDLSDARQRMEMDAYLSQ
                     FPRGGSPAAQAQGQQPQMPQGQQDAQPRQAQQQAEQQQAEQQQPQAPQEQARPATGNG
                     AGQGAGAAGQR"
     gene            3293..3841
                     /locus_tag="VM406_07370"
     CDS             3293..3841
                     /locus_tag="VM406_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HVL75820.1"
                     /translation="MQYSEQDLSALLDALGITPAILAYKALPPYPVATELVVAESDGN
                     GRQFLLIPEAARAWSTMKLAAQADGVVMEIVSAFRDIERQADIIRTKLAKGMAIDHIL
                     TLSAPPGYSEHHTGRAIDINTPGCEPTEEPFEHTDAFRWLCAHAGRFGFTLSYPRDNA
                     LGFIYEPWHWCFQPGHVAGGQT"
     gene            3838..4422
                     /locus_tag="VM406_07375"
     CDS             3838..4422
                     /locus_tag="VM406_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HVL75821.1"
                     /translation="MKLRMAENSLFAILLRSPWWVSFAVAGVIGLLAVALLPRTYAIY
                     GMAGGFPFVVVGTIAMVRQLRAPSAAQVSETLQALAAMPWNEFAATVEQAFRLRGYEV
                     RRSAHPGADFEASDGTRRAVISCKRWKAASHGTEPLRELQRSRDAGDAQEAIYIAIKP
                     PSTQAARLARDTRIELIQDVALVQLVLPALRARA"
     gene            complement(4423..>4624)
                     /locus_tag="VM406_07380"
     CDS             complement(4423..>4624)
                     /locus_tag="VM406_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HVL75822.1"
                     /translation="YRWRRIGENVGSGQGDAARAVSGWLRSPGHCSNIMNPAYTEMGA
                     AYALNRDGEALIYWTQVFGRPR"
BASE COUNT          753 a         1646 c         1467 g          758 t
ORIGIN      
        1 gaacgggcgc cggcactcag cagactcagt aagcagagtc cctcggccgg cgtccgccgg
       61 ccagcagcgc agaacggcca ccttcgggtg gccgttctgc gttctccgtt ctgcgttctg
      121 cgtcccgcgc tcaggcggcc cgtgactgga ccgggtcgat cgcatgctgg ggcggctcgg
      181 ctgcgaccgg ctcgagcagg tcgccgcgca ccatctcgcg catccagttc accagcaagg
      241 ccgtgtacgc acgttgagat gcatcgtcgg aaagtccgtg atcggcccgg gagatcacgc
      301 gataggtcag cgaacgggcg cgggagcagg cctcgacata gctggtgatg accggatgcg
      361 ggatcacctt gtcgcactcg gactcgacca ccagcacatc tcccgcaaag cgcgaacacg
      421 cccgcagcgc acgattgtct ccgggcggca ccacgctgcg gcggtagatc tccagatcgt
      481 cgcccttgtg cagctggcgc ttcggcagat gccagccgtc gtccggatag agcgcgggtg
      541 cgcgcagcgc cagccaccgc accgggcgca gcgcggtcag catcgcggcc atgtagccgc
      601 cgtagctgct gcctaccacg gcaatcgcgt cggggtcggt ctgggggtgg ctggcgagca
      661 catcgtaggc ggcgagcacg tcgcgcaagt tgctctcgcg ggtgacggtc tcgtgctgcc
      721 cgtgcgtgcc ggcatggccg cgcagatcga aggtcaggca aaagcagcca agcgcggcga
      781 tctcgtgtgc gcgagccagg tactgctcct ggctgccgcc ccaaccatgc acgaacagca
      841 cgccagggag ccgcggtgca gggcggacca gcgtgccggc aatccgcgcg ctgtccacat
      901 gaatgtcgat cgactcatcc cgcgtcgcca tatccctcta tgcgtgcata tttggtgatc
      961 cggccgacct tcgggtcgac accggagaac agcacctgcg cgtcttccgg caccggcggc
     1021 gcttcgccgt agacttcgat cgtcgttgcc cgcaccgcct gcagcgcggg atcgccaaga
     1081 aacgcggcga aggccaacag ttccgccccg ctcgccccac cgatgcgcca cgactgctcg
     1141 agcacgccgg acagccagtt gccctcatca tcgcgcccct gcgcaatgtc gtaattgcag
     1201 cgcgacgcgc acaatcccgg aaagctttcg agcgccgccg cgtgatagtg ctgcgcctgc
     1261 tccagcgcga tccgcacttc gtccggcaaa acccgcgcgc gcagcacatc gaattcgccg
     1321 cgcacgacga acaattcgga gccgccgtag acatgccgac cgatgttgtc ggtagtcagc
     1381 cgctggcggc cgtagtagct catcaccagg tcgtcgatcc gggtccggcc ggcgctgagg
     1441 gtgtcgacct gttccaggtt cgtctccagc acgacgcccg gaccaaaacc cggcccggct
     1501 tcaatgctcg ccagtacatc ttccaattcg accgcagtcc gtgctaccga ttggccgaca
     1561 ccgccgacgc cgccgcccag cttgatgcgc accgagccgc attcaagcag gcgccggccc
     1621 gccgcgcgtg cgtcgtcgag cacgaaggcg ctatagcccg gcagcacgac gctgcgcacg
     1681 cgctgaccga agccgtggga ccagccaagc ggcgcctgcg cgccaaggtg cacaagcgga
     1741 tgcgaaatcg tcttcgtggc gacgaatgga aaaggtacga cgccaccgaa cagatcatgt
     1801 tccccgtgca cgcccagacg cgccgcggcc ccggcttcca gcgtgtcgcc cggaacgaaa
     1861 taggcgcgag ccgcgcaatg ccgctccggg tcgtagtccc cgccgaacgg ccagcctttc
     1921 aactcggcca atgcggccgc gagcgcacgc tgcgtcgcgc attcatgccc ggtgggattg
     1981 acacccggcc gggccaggta caccacctct ctcgcgccgt cccggcgccg gttcaaggat
     2041 gtcattgccg ctaccccgcg acttgctgcc ggcgaagctc gccgtgaagc aagcgcagca
     2101 ataagcgggc caactggcgg ccaccccggt atgcccccgg gcggactcag cgctggccgg
     2161 ccgcccccgc tccttgtcct gcgccattac cggttgcagg ccgggcctgc tcctgcggcg
     2221 cctggggctg ctgctgctcg gcctgctgct gctcggcctg ctgttgcgcc tgccgtggct
     2281 gcgcatcctg ctgtccctgc ggcatttgcg gctgctggcc ctgagcctgc gcagcagggc
     2341 tgccgccgcg cggaaactgc gacaggtagg catccatttc catgcgctga cgcgcatcgc
     2401 tcaggtcggg ccagttgtcg cgatcgaatg cgcgcgcctg ctcgacacgc tcgcgatcaa
     2461 gcaccagaaa gatcgggatg tcgggccgcg tcgggaagcg gaaggcagca atcggcaagg
     2521 gcaccagatg ccccccgccg atgccgaagc gggtcgcact gtagtcaagc accgcgtaac
     2581 gcaaatgccc attgccgagg ttgaccacaa tgtcctcgat cgaaccggca tggcgcccgc
     2641 cgccgtcatt tacgcgcttg ccgatcacat ccgatgcgcg cgcgaggcgg ctgcccgacg
     2701 gcttgcggat cacttcgtcg tcctgccaga agaagcggtc cacctgcccg cggtagcttt
     2761 cctcgttcca gtccggccac tcgcgcgcat cgaagcgcgg cgcctgttcc agctgctgct
     2821 ggctgacgtt gagcatgact tccccctcct cgcggccggg ccggaacatg accgggggga
     2881 aggcggccat ctggttgccg atgccgagaa cgcctccgtg cgagacgatc gcgtagcgca
     2941 cctgggaatt ggcgacatcg atgatcaagt cctcgatccg cccaagatcc tgtccctgcg
     3001 gattcgtcac gtccttgccg atcagttcgc tcgcgcgcac atcgagcagg gcgagctgct
     3061 cggccgtgag cggtggtgcg ccggccgtcg gcgcctgctg ctgctgctgc gcgaccgcgg
     3121 ccgtgccgaa cgcgagccat ggcaccagga tgccggcggc gagtttccga tgcaaagtgt
     3181 tcatgttgcg ctcctcatgc ttatctttat gtaatgactg gccgcgccgc catcggttcg
     3241 cgtgatgctg gcgtacaatt cggcttcctt cgcccgtgtc aacctctttg cgatgcagta
     3301 ttcagagcaa gacctctccg cccttctcga tgccctcggc atcacgcccg ccatcctcgc
     3361 gtacaaggcg ctcccgccct atccggtcgc cacggaactc gtcgtcgcgg aaagcgacgg
     3421 caacggccgg caatttctgc tcattccgga ggcggcacgc gcctggagca ccatgaagct
     3481 ggccgcgcag gccgacggcg tggtgatgga aatcgtctca gcgttccgcg acatcgagcg
     3541 ccaggccgac atcatccgta ccaaactcgc aaaaggaatg gcgatcgacc acatcctgac
     3601 cctgagcgcg ccgccgggct acagcgagca tcacaccggc cgtgccatcg acatcaatac
     3661 cccgggctgc gagcccaccg aggaaccgtt cgaacacacg gacgcgttcc ggtggctgtg
     3721 cgcgcatgca gggcgcttcg gctttacgct gtcctacccg cgcgacaatg cgctgggctt
     3781 catctatgaa ccgtggcact ggtgtttcca gcccggccac gttgccggag gacagacatg
     3841 aagctgcgca tggccgagaa ttccctgttc gccatcctgc tgcgctcgcc ctggtgggtc
     3901 agttttgcgg tcgccggcgt catcggcttg ctcgccgtcg cgcttcttcc ccgcacctat
     3961 gcgatctacg gcatggctgg cgggtttccg ttcgtcgtcg taggcacaat cgcgatggtg
     4021 cgccagctgc gcgcgccgag cgctgcccag gtcagcgaga cgctgcaagc gcttgcggcg
     4081 atgccgtgga acgaatttgc ggcgaccgtc gagcaggcgt tccggctgcg cggctacgaa
     4141 gtgaggcgca gcgcccatcc gggggcggac tttgaagcga gcgacggcac ccgccgggcg
     4201 gtgatcagct gcaagcgctg gaaagccgcc agccacggaa ccgagccgct gcgcgagctg
     4261 cagcgctcgc gcgatgcagg cgacgcgcag gaagccatct acatcgccat caaaccccct
     4321 tccacccagg cagcccgcct ggcgcgcgat acccgcatcg agctgatcca ggacgtcgcg
     4381 ctggtgcagc tggtgctgcc ggcgctgcgc gcgcgtgcct gatcagcgcg gccggccgaa
     4441 gacctgcgtc cagtagatca aggcctcgcc gtcccggttc agcgcatacg cggcgcccat
     4501 ctccgtgtac gccggattca tgatgttgct gcaatgcccc gggctgcgca gccagcctga
     4561 caccgcacgg gccgcgtcgc cctgcccgct tccgacgttc tcgccgatcc gtctccagcg
     4621 atag
//
LOCUS       DATLGF010000244         7677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_26594,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000244 DATLGF010000000
VERSION     DATLGF010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 7677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7677
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26594"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..1730
                     /locus_tag="VM406_07385"
     CDS             60..1730
                     /locus_tag="VM406_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HVL75823.1"
                     /translation="MKFSFNLKIGTRLALAFGAVMFLMTLLAATSIWALNEVGRVTDH
                     MARHSFPKERLVREFAGQIELNGARILAGARNADPEQQKFFKDQIAATIATNNELVKQ
                     ISELLSTDAEKALFAEIGERRKVVIAANNAVFAEKDAGNEETAQHLLRTRLEPELRDY
                     IATIKKLAVHEHDVIQQHVSDVEQTNRSWGRWIILLTLAGIIAGIAFAYRITRSITRS
                     FGQAVETARRVADGDLSANIEVTSRDETGQLMQALKDMNDGLQKIVTEVRAGTASLTT
                     ATAEIAAGNQDLSARTEQQASSIEETASSMEELTSTVKQNAENARQANQLAATASEVA
                     TKGGTVVSEVVDTMGSINESSKKIVDIIGVIDGIAFQTNILALNAAVEAARAGEQGRG
                     FAVVASEVRSLAQRSAAAAKEIKALIDSSVEKVDAGTRLVGEAGATMDDIVNSVRRVT
                     DIMAEIAAASAEQTAGIEQINQAIAQMDQVTQQNASLVEEAAAASEAMQEQATKLTQV
                     VSVFKVSGQPATAAVAQRTASKHAGRPVAAPARKPLPVPARGSDWEEF"
     gene            1966..3147
                     /locus_tag="VM406_07390"
     CDS             1966..3147
                     /locus_tag="VM406_07390"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HVL75824.1"
                     /translation="MHHFLTGTARIGLALTLCGAFLLPSAFAQIEPTGTPPDAKGWRT
                     ATVVEGIRNPWGMTWLPDGRLLITGKYGTLHMVENGQLRDIAMDGLPEVFTEGQGGLL
                     DIALHPNFQQNQRIYLTLSTGTKDANRIILVRGTFNGQRVSNIETLFRVQPDKDGDEH
                     FGSRLLWLPDGTMLMTIGDGGNPPRRIGDMLAREQAQNLQSHLGKILRLTEDGKPAPD
                     NPFAGKQGVRPEIWVYGVRNIQGITREPESGRVWANEHGPRGGDELNLIEGGRNYGWP
                     TQTWGVDYRTGEPFGEKSVEGMPDPKVVWTPAHAPSGLAYYTGDRFPQWRGSLFSGGL
                     ASEDIRRIELDGNGKVVNQDRLDIGRRVRDVRQGPDGYLYAITDEENGRLLRIEPDQG
                     S"
     gene            complement(3170..3508)
                     /locus_tag="VM406_07395"
     CDS             complement(3170..3508)
                     /locus_tag="VM406_07395"
                     /inference="COORDINATES: protein motif:HMM:NF025045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4148 domain-containing protein"
                     /protein_id="HVL75825.1"
                     /translation="MQTRTWMTAAAFACAFAAAGPTQAEVGRPNGELPPTDYFSSRMS
                     RADVARELADSRAAGLTGTDTDLIDSRGTGFRADMIAADGRAQSPQPRVQPQMAGSPD
                     RPASEDPYAG"
     gene            complement(3937..4626)
                     /locus_tag="VM406_07400"
     CDS             complement(3937..4626)
                     /locus_tag="VM406_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019704049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HVL75826.1"
                     /translation="MITVHHLETSRSQRVLWLLEELGLPYEIMRYARDPKTRLAPPEL
                     KKVHPLGKSPVVIDEGVTVAESGAILEYLAERYGAQAQGDLSDLQPPAGTDEYRQCRF
                     WMHFAEGSLMNWLVMKLVFVTIPTQPMPFFVRPIARQLCTNVQSRLIDPNLATALAFI
                     DDHLGRHAWFAGDAITIADFQMSFAVEAALARSKDAGRYPRLRAYKEKMEARPAYQRA
                     LAKGGPVVMQA"
     gene            complement(4655..5011)
                     /locus_tag="VM406_07405"
     CDS             complement(4655..5011)
                     /locus_tag="VM406_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CZB domain-containing protein"
                     /protein_id="HVL75827.1"
                     /translation="MNLDEALIRDGELRNKLLAAIAGKHQLDAKAVAKLDGCALGNWL
                     HGEAERKYPFVKSYRPAVDAHAAFHAEMEKVVRQINLEEYAQAEAMLASGTPCSRAFV
                     AMVAAVRQLKKDARLD"
     gene            5254..5526
                     /locus_tag="VM406_07410"
     CDS             5254..5526
                     /locus_tag="VM406_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75828.1"
                     /translation="MDETLLSLFGLLAGLAAGLLAGKLVARVIPYAREAAPQHFAGAT
                     ATLLMLAAAGLALGYFIRIFLDHWSALAGALAAFLYFITPLLKSDP"
     gene            complement(5530..6795)
                     /locus_tag="VM406_07415"
     CDS             complement(5530..6795)
                     /locus_tag="VM406_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL75829.1"
                     /translation="MTERSTASWYFGWNIVAAATVLTLLTVGMRLSIGPFFLPMAEDL
                     GFSRSLLAGIIAIGMLFYGIGMPLAGYLVNRHGTAFVLLLGVAIVAVSTVWTVTARSP
                     LAFLLAFGVLMSIGLAFTSPVALTPVISRWFTRRRGMALFFLSTGSMAGMALMTPALT
                     FAIESTSWQTTLLGFAAAFALVTVPVALFIVRDDAPVHTDLLPDQIAAARAAPAAPPQ
                     NLRFTEAIRTPPFLKICLGLFACGFSMNLLGTHGVPMLMDHGFDAMTSSYGIGLIGLV
                     AIFSTMVLGRLSDILPRRHILAAIYLIRGLGFFVLLLVGTHWELYAAATIGGIVWAGS
                     IALSSAILADVYGVRLVGILYGWAYLGHQVGAMISSWLGGWGYETWGTHWVAFGSSGV
                     MLLMAAAVSLRLPRKGFTLTAPPLAVGRG"
     gene            complement(6913..7539)
                     /locus_tag="VM406_07420"
     CDS             complement(6913..7539)
                     /locus_tag="VM406_07420"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HVL75830.1"
                     /translation="MMRTALLLTCLLGVTSSAMSQGHEVAGCRIRTGTLCADRNMNGV
                     DLKGANLARAQLSRSTLVGADLEQADLTNANLTRVDLTRANLQRAVLNDANLLGARLT
                     EARLGQAQLSRANLRNAVLRETDLREAVLQAADLLSANLSGAQMQKADLQRADFRTAN
                     LSGASLAGARLNGANFTKANLSNADLRGADLEGANFSGALTTGCHGCP"
BASE COUNT         1431 a         2428 c         2547 g         1271 t
ORIGIN      
        1 cgcccgcacg gcgcgtaccg ccgctcgcct ttccgtttcc aactctgcac tcgttctcca
       61 tgaaattcag cttcaacctg aagataggca cgcgcctcgc gctcgccttc ggcgccgtga
      121 tgttcctgat gacgctgctg gccgcaacca gcatctgggc gctcaacgag gtcggccgtg
      181 tgaccgatca catggcccgc cactccttcc cgaaggagcg gctggtgcgc gagttcgcag
      241 ggcagatcga actcaacggc gcgcgcatcc ttgccggcgc gcgcaacgcc gatcccgagc
      301 agcagaaatt cttcaaggac cagattgcgg cgaccatcgc aaccaacaac gaattggtga
      361 aacagatctc cgaactgctt tccaccgacg cggaaaaggc cttgttcgcg gaaatcgggg
      421 agcggcgcaa ggtcgtgatc gcggcgaaca atgcagtgtt cgcggaaaag gatgcgggca
      481 acgaagagac tgcccagcat ctgctgcgca cccgtcttga acccgaactg cgcgattaca
      541 tcgcgacgat caagaaactc gccgtgcatg aacatgacgt gatccagcag cacgtcagcg
      601 acgtcgaaca gaccaacaga agctggggcc gctggatcat cctgctgacc ctcgccggca
      661 tcatcgccgg catcgccttt gcgtaccgga tcacacgttc gattacccgc tcgtttggcc
      721 aggccgtcga gacggcgcgc cgcgtcgccg atggcgacct gagcgcgaac atcgaagtga
      781 cctcgcgcga cgaaaccggc cagctgatgc aggccttgaa ggatatgaac gacggtctgc
      841 agaagatcgt caccgaggtg cgcgcgggca ccgcctcgct gacgacggcg acggcggaga
      901 tcgcggccgg caaccaggac ctgtcggcgc gcaccgaaca gcaggccagc tcgatcgagg
      961 aaaccgcatc gtcgatggag gagctcacat ccaccgtcaa gcagaacgcg gaaaacgcgc
     1021 gccaggccaa ccagcttgcg gcgaccgcgt cggaagtggc caccaagggc gggacggtgg
     1081 tctccgaggt ggtcgatacg atgggctcga tcaacgaatc atcgaagaag atcgtcgaca
     1141 tcatcggcgt catcgacggg attgcgttcc agaccaacat cctggcgctg aacgcagcgg
     1201 tggaagccgc gcgtgcaggc gagcaggggc gcggcttcgc ggtggtcgcg tcggaagtgc
     1261 gcagccttgc gcaacgctcg gccgccgcag ccaaggagat caaggcactg atcgacagct
     1321 cggtcgagaa ggtcgatgcc ggcacgcgcc tggtcggcga ggccggggcg acgatggacg
     1381 acatcgtcaa cagcgtgcgc cgcgtgaccg acatcatggc cgagattgcg gccgccagcg
     1441 ccgagcagac cgccggcatc gagcagatca accaggcgat cgcgcagatg gaccaggtga
     1501 cccagcagaa tgcttcgctg gtggaagagg cggctgccgc atccgaggcg atgcaggagc
     1561 aggcgacgaa gctgacgcag gtggtttccg tgttcaaggt gagcgggcag ccggcgactg
     1621 cggcggtcgc gcagagaacg gcaagcaagc acgccggcag gccggttgcc gcgcccgcgc
     1681 ggaaaccgct gcccgtgccc gcgcgtggca gcgactggga agagttctga tacgggcgca
     1741 cctcacgccg tgtatctgta cggcaccgcg cacggtatca gcgcgacgac agggtagccg
     1801 gcgctgttca gccggcaccc gccccacgca ggatcgccaa ccggcatacc cgtgagacgg
     1861 tccagtctct gacgcagatc agtgacaggt tccaggttcg ctgcaactca ggccggatgc
     1921 cttgactcct acccttcgcc caatcggaca agaacggaga acaagatgca ccacttcctg
     1981 acgggaactg cccgtatcgg gctggcgctg acgttgtgcg gcgccttcct cctaccttcc
     2041 gcctttgccc agatcgagcc gaccggcacc ccgccggacg ccaagggctg gcgcacggca
     2101 accgtcgtcg agggcatacg caacccatgg ggcatgacct ggcttcccga cggccgcctg
     2161 ctcattaccg gcaaatacgg aacgctgcat atggtagaga acgggcagtt gcgcgacatt
     2221 gcgatggacg ggcttcccga agtcttcacc gaggggcagg gcgggttgct ggacatcgcg
     2281 ctgcatccaa acttccagca gaaccagcgc atctacctga cgctgtcgac cgggaccaag
     2341 gacgccaatc gcatcatact ggttcgcggc acgttcaacg gccagcgcgt gagcaatatc
     2401 gaaaccctgt tccgggtcca gccggacaag gatggcgacg agcacttcgg ctcgcggctg
     2461 ctctggcttc ccgacggcac gatgctgatg accatcggcg acggcggcaa tccgccgcgg
     2521 cgcatcggcg acatgctggc gcgggagcag gcgcagaacc tgcaaagcca tctcggcaag
     2581 atcctgcgcc tgaccgaaga cggcaagccg gcgccggaca atcctttcgc cggcaagcaa
     2641 ggtgtgcgtc cggagatctg ggtctatggc gtgcgcaaca tccagggcat cacgcgcgag
     2701 ccggagagcg gccgcgtctg ggccaacgag catgggccgc gcggtggcga tgaactgaac
     2761 ctgatcgagg gcgggcgcaa ctacggctgg ccgacgcaga cctggggcgt cgactaccgg
     2821 accggcgaac cctttggcga gaagtcggtg gaagggatgc ccgacccgaa ggtcgtgtgg
     2881 acgccggcgc atgcgccttc ggggctcgct tactacaccg gcgatcgctt cccgcagtgg
     2941 cgcggcagcc tgttcagcgg cggactggcc agcgaagaca ttcggcgtat cgaactcgac
     3001 ggcaacggca aggtcgtgaa ccaggatagg ctcgacatcg ggcgacgcgt gcgcgatgtg
     3061 cgacaggggc cggacggcta tctgtacgcg atcaccgacg aggagaacgg acggctgctg
     3121 cgcatcgagc cggaccaggg cagctgatgc cgccctggtc cgacggcggt cagcctgcgt
     3181 acgggtcctc cgacgccggg cgatcgggcg atccggccat ctgcggctgc acgcgcggct
     3241 gcgggctctg agcacggccg tcggccgcga tcatgtcggc acggaagccg gttccgcggc
     3301 tgtcgatcag gtccgtgtct gttccggtca ggccggcggc gcgactgtcg gcaagttcac
     3361 gcgcgacatc ggcgcgcgac atccgggaac tgaagtaatc agtgggagga agttcaccgt
     3421 tcggtcggcc gacctccgcc tgcgtggggc cggcggctgc gaacgcgcaa gcgaatgcgg
     3481 ccgcagtcat ccatgtgcgg gtttgcatga tgctctccaa tggcaattca tggcctgtca
     3541 tcgaatggcc atcccgcgag cgtgcgtcgc gcatgcggcg tatcgtctgc aggacagcct
     3601 tcgtcctgct tgcggctttt ccgcaagttt cgatgcggtg atatcgctgc ctggcccgga
     3661 agcgatcttc gcgcaacgtg tcaccgactg gcggagaacg aggaagcggg ggcgcgcatt
     3721 tctggtgcgg gtccctcgtc acgatcatgg cgcaacgagc gtccctggcc ggaccgccga
     3781 gatgaaacct ggtaaggagc cagtgccggg tcagtatatg tcacacgttt gtgacagcaa
     3841 gcggagattt gcgatggctg cgcaaactgc cattcatctg ttcgcgcccg cccctgccga
     3901 agtggaaagt gttccgacat cagctttccg tgcagttcat gcctgcatca cgaccgggcc
     3961 gcccttggcc agcgcgcgct ggtaggcggg ccgcgcttcc atcttctcct tgtacgcgcg
     4021 caggcgcgga tagcggccgg cgtccttgct ccttgccagt gccgcctcca ctgcgaagct
     4081 catctggaaa tccgcgatcg tgatcgcgtc gccggcgaac caggcgtgcc gtccgaggtg
     4141 atcgtcaatg aacgcgagcg ccgttgcgag attgggatcg atcagcctgg actgcacgtt
     4201 cgtgcagagc tggcgcgcga tgggacgcac gaagaagggc atgggctggg tgggaatcgt
     4261 gacgaacacc agtttcatca ccagccagtt catcagcgag ccttccgcga agtgcatcca
     4321 gaaccggcat tgcctgtatt cgtcagtgcc cgcgggcggc tgcagatccg acagatcacc
     4381 ctgcgcctgc gcgccgtagc gctcggccag atattcgagg atcgcacccg attcggccac
     4441 cgtgacgcct tcatcgatga cgactggcga cttgcccagc gggtgcactt ttttcagctc
     4501 gggcggcgcg agccgtgttt tcggatcacg cgcatagcgc atgatctcat acggcaggcc
     4561 aagctcttcg agcaaccaga gcacgcgctg cgaacgggac gtctcgagat ggtggacggt
     4621 gatcatggtt ctcctcgctg tcgtgtcgcg tgtcctaatc gagtcgcgca tccttcttca
     4681 actggcgtac cgcggcgacc attgcgacga aggcgcgcga gcagggcgtg ccgctggcga
     4741 gcatggcttc ggcctgcgcg tattcctcga gattgatctg gcggaccacc ttttccatct
     4801 ccgcgtggaa cgccgcatgg gcatcgaccg ccgggcggta gctcttcacg aacggatact
     4861 tgcgctcggc ctcgccatgc agccagttgc ccagcgcgca gccgtcgagc ttggccacag
     4921 ccttcgcgtc gagttgatgc ttgccggcga ttgccgcgag cagcttgttg cgcagttctc
     4981 cgtccctgat cagcgcttca tcgagattca tggtgtcctt ccgggtttgg gtccgacgtc
     5041 ccattgcatg gaaccgatgg aaacggactt catcacaagt gggcgggatg tgcaagcgcg
     5101 caccggaagc gaagcaggaa aaggcaacaa catgcaccgg ctcgttcatg catcaaaggc
     5161 gtcaagccgt cggcatgcgc gggatcacgg tgcacgcgca tcagccggct agtttgcatg
     5221 catggccaaa aaacgcagac ccgggagggc gcattggacg aaacacttct ttcgctgttc
     5281 ggcctgcttg ccggcctggc ggcgggtctc ctggccggga agctcgttgc ccgggtgatc
     5341 ccatacgccc gggaggccgc gccgcagcac ttcgccggtg cgacggccac gctcctgatg
     5401 ctcgctgcgg ccggtcttgc gcttggctac ttcatccgta tctttctcga ccactggagc
     5461 gcgctggcgg gtgcgcttgc ggccttcctt tacttcatca cgccgttgtt gaagtcagac
     5521 ccgtagcgct catccgcgac caacggcaag tggcggcgcc gtcagcgtga agcccttcct
     5581 cggcaggcgc agcgagaccg cggcggccat cagcagcatg acgccggacg acccaaatgc
     5641 gacccagtgc gtgccccagg tctcgtagcc ccatccgccc agccacgagc tgatcatggc
     5701 gccgacctgg tggccgagat aggcccagcc atacaggatg ccgaccaggc gcacgccata
     5761 gacatccgcg aggatcgccg acgacagggc gatgctgccg gcccagacga ttccgccgat
     5821 ggtggccgct gcatacagtt cccagtgcgt gcccacgagc aggagcacga agaagccgag
     5881 gccgcgaatg agatagatcg cggcaaggat gtgccggcgc ggaagaatgt ccgacaggcg
     5941 tcccagcacc atcgtgctga agatggcgac caggccgatc aggccgatgc cgtacgagct
     6001 cgtcattgcg tcgaagccat ggtccatcag catcggtacg ccgtgcgtgc cgagcaggtt
     6061 catgctgaag ccgcaggcga acaggccgag gcagatcttc aggaaggggg gcgtgcgtat
     6121 cgcttcggta aagcgcaggt tttgaggggg cgcggccggt gctgcgcgcg ctgctgcgat
     6181 ctggtcggga agcaggtcgg tatgcaccgg cgcatcgtcg cggacgatga agagcgcgac
     6241 cggaacggta accagcgcaa aggcggcggc gaagccgagc agggtggtct gccagctcgt
     6301 cgattcgatt gcaaaggtca gcgccggcgt catgagtgcc atgccggcca tcgatccggt
     6361 cgagaggaaa aacagtgcca tgccacggcg ccgcgtgaac cagcggctga tgacgggagt
     6421 gagcgcaacc gggctggtga aggccaggcc gatggacatc aggacaccga aggccagcag
     6481 aaaggcgagc gggcttcgtg cggtgacggt ccagaccgtg gacacggcga caatcgccac
     6541 gccgagcagg agcacgaacg ctgtgccgtg acgattgacc agatagccgg ccaatggcat
     6601 gccgatccca tagaacagca tgccgatggc gataatgccc gccagcaggc tgcggctgaa
     6661 accgaggtct tccgccatcg ggaggaagaa cggacctatg ctcagccgca tgccgacggt
     6721 gaggagggtg aggacggtgg cggctgccac gatgttccag ccgaagtacc agctggcggt
     6781 ggatctctcg gtcactgcgg catctcctct cgatcgaatg cagcgccaac gcgctggctg
     6841 cggaccgaga gattgtccgc tattttccgc agcccggcag gcgcgccggc cgcggccgcc
     6901 ggagctcttg tgtcatgggc agccgtggca gccggtggtg agcgcgcctg aaaaattcgc
     6961 gccttccagg tccgcgccgc gcaggtcggc gttgctgaga ttggccttgg tgaagttggc
     7021 gccgttgaga cgggcgccag ccagtgatgc gcccgacaga ttcgcggtac gaaaatctgc
     7081 cctttgcaga tcggccttct gcatctgcgc gccggagaga ttggcggaga ggaggtctgc
     7141 ggcctgcagc acggcttcgc gcaggtcggt ttcgcgcagt accgcattgc gcaggttcgc
     7201 acgactgagc tgcgcctgtc ccaacctcgc ttcggtcagt cttgcgccga gcaggtttgc
     7261 gtcgttgaga accgcgcgct gcaggttggc gcgggtgagg tccacccgcg tcaggtttgc
     7321 attcgtgaga tcggcctgtt cgagatccgc gccgaccagc gtggagcgcg agagctgcgc
     7381 gcgggcgagg ttcgcgcctt tcaggtcgac gccgttcatg ttccggtccg cgcacagtgt
     7441 gccggtgcgg atgcggcagc ctgccacttc atgtccctga ctcatggccg atgacgtcac
     7501 gccgagcagg caagtcagaa gcagggcggt tctcatcatt cgggtgtctc catgtgtttc
     7561 gttcgcaggc aagcttttcc gcctgctcgg tcaggcgtcc ccaagggcag cccaaggata
     7621 gcggaacacc cgctgcctgt gtctcaaagc aatgccgcaa gcgcctgcaa taccgta
//
LOCUS       DATLGF010000245         2533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_42707,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000245 DATLGF010000000
VERSION     DATLGF010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42707"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VM406_07425"
     CDS             <1..183
                     /locus_tag="VM406_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75831.1"
                     /translation="GTSGMTDQGQFAPQSWMAGFPGVGDGGPSMGIGGGAPYPYPMFG
                     QMPFERMPRVFGRPVW"
     gene            complement(464..793)
                     /locus_tag="VM406_07430"
     CDS             complement(464..793)
                     /locus_tag="VM406_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009871327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit G"
                     /protein_id="HVL75832.1"
                     /translation="MHFAVELLVSVFLLAGAAFALIGSWGLVKLPDFYMRLHGPSKAS
                     TLGVGGMLIASMIYFLAQEGELSLHELLITLFLFITAPVSAHMVAKAALHTKLKSVTP
                     PPADHKE"
     gene            complement(803..1072)
                     /locus_tag="VM406_07435"
     CDS             complement(803..1072)
                     /locus_tag="VM406_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HVL75833.1"
                     /translation="MLTTAIWIASAAIGLALLLSLYRLLRGPDVPDRIIALDTAYVNA
                     TALLVLFGVQQNSTLYFEAALLIAMVGFVGTAMLAKYMLRGDIIE"
     gene            complement(1066..1560)
                     /locus_tag="VM406_07440"
     CDS             complement(1066..1560)
                     /locus_tag="VM406_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HVL75834.1"
                     /translation="MMRSRWLPHPLASVTLFAMWLMLNATVAFSHVVLGAFLAWVIPW
                     STRSLWPRQFGLYRGKVALRLFFVVLADIVRANIIVALLILGPTSKLRPGFVRMRVDL
                     DEPFAASTLASIITLTPGTVSCSFSEDRRTLLIHALDMDDEAALIEEIKTRYEAPLKE
                     IFQC"
     gene            complement(1557..>2533)
                     /locus_tag="VM406_07445"
     CDS             complement(1557..>2533)
                     /locus_tag="VM406_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HVL75835.1"
                     /translation="LAVKVAAADAADTALIRSAALIMLVVFSVKAALLPLYFWLPGAY
                     SNAEAPVAALFAIMTKVGLYSILRVFSVAFGPDAGVAADVAAPWLLPAGLITLAVASL
                     GALACRSLRLLVAYMTIASVGTITIGIGLASEAAIAAALYYLVHSTLAISLLFLLSDL
                     IMRGRGLMSDRLARARPVPQRELLGSLFFFGAVAVVGVPPLSGFFGKVLLLHGSQDHA
                     SMAWVWAVVLATALIGMIAFARAGSTLFWHTDEAAGARGQHVSARATPMELVPPFVLV
                     ACIVAMTVWAQTVADYSVGAARQLLQPSQYIHAVLGSPVPSTMQEETK"
BASE COUNT          522 a          801 c          852 g          358 t
ORIGIN      
        1 ggcacgagcg gaatgaccga ccagggccag tttgcgccgc agtcgtggat ggcgggattt
       61 ccgggcgtgg gggatggggg gccgtcaatg ggaatcggcg gcggcgcgcc ctacccttat
      121 ccaatgtttg gacagatgcc gttcgagcgg atgccgaggg tattcgggcg cccggtctgg
      181 tagatctgca aaaaccttga accacgcgaa cacggcgaaa acccctgtca ttccgagcac
      241 agcgaggaat ctccgctgca tgaggcgatg gggtccctcg ctgtgctcgg gacgacaggc
      301 agtgggattt tcgccatgca tcgccgtgct cgccgtggtg aaaggcagcg ttaaacgtac
      361 cgaaccacgg cgggcccggc gaaaaagcct gccgttccgg gatcgcgcgg ggacaacatg
      421 gcgagggcaa gcgctcaagc gaacggcgct tgcaccgttg cgcttactcc ttgtgatcgg
      481 cgggtggcgg cgtgacggat ttgagcttcg tgtgcagcgc ggccttcgcc accatgtgcg
      541 cactgaccgg ggcggtgatg aagaggaaca gggtgatcaa cagctcatgc aggctgagct
      601 cgccttcctg ggccaggaaa tagatcatcg aggcgatcag catgccgccc acgcccagcg
      661 tggacgcctt ggacggcccg tgcaggcgca tgtagaaatc cggcagcttg accagtcccc
      721 aagagccgat cagcgcaaac gcggcgccgg caagcaggaa gacggaaacc agcagttcga
      781 cggcaaagtg catgtttccc ccttattcga tgatgtcgcc gcgcagcatg tacttcgcca
      841 gcatcgcggt accgacgaaa cccaccatcg cgatcaggag cgccgcttcg aagtacagcg
      901 tgctgttctg ttgaacgccg aacagcacca gcagcgcggt tgcgttcaca taggcggtat
      961 cgagcgcgat gatgcggtcg ggcacgtcgg ggccgcgcag caggcggtac aggctgagca
     1021 gcagggccag gccaatggcg gccgaggcga tccagatggc ggtcgtcagc attggaaaat
     1081 ctccttcagt ggcgcttcat agcgcgtctt gatttcctcg atcagcgccg cttcgtcgtc
     1141 catgtccagc gcatggatca gcagggtgcg gcggtcttcg gaaaacgagc aggacacggt
     1201 gcccggggtg agcgtgatga tgctggccag ggtcgaggcg gcgaacggtt catccaagtc
     1261 gacgcgcatg cgcacgaagc ccggacgcag cttcgaggtc ggccccagga tcagcagcgc
     1321 gacgatgatg ttggcccgca cgatgtcggc cagcacgacg aaaaacaggc gcagggccac
     1381 cttgccgcgg tacaggccga actggcgcgg ccacagcgaa cgcgtggacc agggaatgac
     1441 ccaggcgaga aaggcgccga gcacgacatg cgagaacgcg acggtggcgt tgagcatcaa
     1501 ccacatcgcg aacaaggtga ccgatgccag cgggtgcggc aaccagcgac tgcgcatcat
     1561 ttcgtttcct cctgcatggt cgacggcacc ggcgaaccga gcaccgcgtg gatgtattgg
     1621 gacggctgca gcaactgccg cgcagccccg accgagtagt cggccacggt ctgcgcccat
     1681 accgtcatcg cgacgatgca agccaccagc acgaacggcg gcaccagctc cattggcgtt
     1741 gcgcgcgccg acacatgttg tccgcgtgcg cccgctgctt catcggtatg ccagaacagc
     1801 gtgctgccgg cccgggcaaa ggcgatcatg ccgatcaatg ccgtcgccag cacgaccgcc
     1861 cacacccagg ccatcgaggc atggtcctgc gagccgtgca gcagcagcac cttgccgaag
     1921 aagccggaca gcggcggcac ccccaccacg gcgaccgcgc cgaagaagaa cagcgaaccc
     1981 agcagttcgc gctgcggcac cgggcgcgca cgcgcaagac gatcgctcat caggccgcgc
     2041 ccgcgcatga tcaggtcgga cagcaggaac agcaaagaga tcgccagcgt actgtgcacc
     2101 aggtagtaca gcgcggcggc gatcgccgct tccgacgcca ggccgatgcc gatcgtgatc
     2161 gtgcccacgg aggcgatggt catgtaggcg accagaaggc gcagtgaacg gcaggccagg
     2221 gcaccgagcg aggcaaccgc gagcgtgatc aggccggcgg gcagcagcca aggcgcggcg
     2281 acgtcggccg ccacgcctgc atccggaccg aaggcgaccg agaacacgcg caggatcgag
     2341 tacaggccga ccttggtcat gatcgcaaac agcgcggcaa ccggggcctc ggcattgctg
     2401 tacgcgcccg gcagccagaa gtacagcggc agcagtgcgg ccttgaccga gaacaccacg
     2461 agcatgatca gtgctgccga acggatcagc gccgtgtcgg ccgcgtcggc cgctgccacc
     2521 ttcaccgcca gat
//
LOCUS       DATLGF010000246         4884 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_42708,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000246 DATLGF010000000
VERSION     DATLGF010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42708"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VM406_07450"
     CDS             <1..833
                     /locus_tag="VM406_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HVL75836.1"
                     /translation="TLGLVGESGCGKSTTGRCLVRLLDATSGSVKFDGQELTTMGSGA
                     LRQMRRHMQFIFQDPFSSMNPRMRVRDIIGEPLRNFGADSAKINQRVSELLERVSLRP
                     DAATRYPHEFSGGQRQRIVVARALALQPKLIICDEPVSALDVSVQAQVINLLRDLQRD
                     MNLSYVFISHDLSVVRYISHRVAVMYLGHIVESGPREAVFQSPTHPYTQALLSAVPEV
                     GKRNKTIQILKGEIPSPMSPPSGCTFRTRCPRAQALCAERAPEMKTIGEGHQAACHFV
                     "
     gene            847..1221
                     /locus_tag="VM406_07455"
     CDS             847..1221
                     /locus_tag="VM406_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HVL75837.1"
                     /translation="MSDITRYPSTLPFPFSRAVRAGGFLFLSGQIPMDAEGKVVQGDI
                     TEQTRAVMERIGESLQACGASYADVVKVTVWLSDFSQFAAFNEVYKTYFKDGFPVRSA
                     VEAKLAFNVGVEVEVQAWVGDR"
     gene            1218..2669
                     /locus_tag="VM406_07460"
     CDS             1218..2669
                     /locus_tag="VM406_07460"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HVL75838.1"
                     /translation="MSAQPFDLVLVGGNIIDGTNRPAFKADIGIIGERIVAIGNLAAS
                     PARARRNITGRTVAPGFIDSHTHDDTLLLRKRDMAPKVSQGVTTVVTGNCGISLAPLN
                     LDGGVPPAPMTLLDAGGDFRFGSFSAYMDALKATPPAVNAACMVGHSTLRAAVMGDLD
                     RPATEGETESMRALADEAMRAGAIGLSTGTFYPPAAHATTEEIIEVCRPVAEQGGIYA
                     THMRDEGDKIVEALEETFLIGRSLNVPVVISHHKVMGANNFGRSKETLAIIEKAMAEQ
                     DISLDAYPYIAGSTMLKKDRTLLAAKTIITWCKPFPELAGRDLDEVAAERGTTKWDII
                     DSLQPAGAIYFMMDEADVQRILAFPETMVGSDGLPHDERPHPRLWGTFPRVLGHYARD
                     VKLFPLETAVWKMSGLTAAKFGLKDRGVIRENAFADLVVFDPATIADAADFDHPTRIS
                     PGIDVVFVNGQTIWENGAATGNYPGQVLKREAA"
     gene            2678..3622
                     /locus_tag="VM406_07465"
     CDS             2678..3622
                     /locus_tag="VM406_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoyl-D-amino-acid hydrolase"
                     /protein_id="HVL75839.1"
                     /translation="MSQRLVHLAVAQMGPVQRSHARADAVERLVAMLQEAARRGAQWV
                     VFPELALTTFFPRWDLQDQAEIDAFYETEMPNAATAPLFAAARERKIGFYLGYAELVN
                     GRHFNTSILVGQDGNIIGKYRKIHIPGHASSQTGGAPIQHLEKKYFEVGDLGFPVWQT
                     RDAKIGMCICNDRRWPETWRSMGLQGIEIGMVGYNTPSAFVQWDEPIHLRMHHHLLSL
                     QAAAYQNACWIAASGKAGVEEGSHMIGGSCIVAPTGEVMVKTQGEGDEVISFECDLSI
                     GDRYRANIFNFAAHRRTEHYGLLVERTGAGAPLGTTPE"
     gene            3643..4311
                     /locus_tag="VM406_07470"
     CDS             3643..4311
                     /locus_tag="VM406_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HVL75840.1"
                     /translation="MTMLLRGTPHPGVATDLAERHTESAGTRTLTRPAIASKEEVGRR
                     LRALRKSSKLTLKMLSEASGIPLSTISKMELGQIAVSYEKFSAIAHALNVDPSSLFDG
                     SSASTEARPTVVKSSAASTAEYRSETYIHRMLAGDFPHKKMTPMQAQLLARDRSEFAD
                     FIRHPGEEFVMVLSGTVRIEFETGESITLKRHESAYFNSSIGHIYLSVGRGNAEVVAV
                     CTDK"
     gene            complement(4385..>4884)
                     /locus_tag="VM406_07475"
     CDS             complement(4385..>4884)
                     /locus_tag="VM406_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75841.1"
                     /translation="SAAPESRTAAAKAGRSSAARSGTAVKKAGAKPKSAIKAASTAKA
                     AKADKPAKAAKASGAAPKKKSATRAANKPASKERAAVPTLQRLVRTVAKAAAKIKDEA
                     VDATTAAPSRSAKAPAKADGASTRRTRTKSPVNPKGPEFARNPRERGRVGSVQRRQQA
                     RRDSR"
BASE COUNT          866 a         1658 c         1545 g          815 t
ORIGIN      
        1 agacgctggg cctggtcggc gaatccggtt gcggcaagtc gacaaccggg cgctgcctgg
       61 tgcgcctgct ggacgcgacc tccggttcgg tcaagttcga cggccaggag ctgacgacaa
      121 tgggcagcgg cgcgctgcgg caaatgcgcc gccacatgca gttcatcttc caggacccgt
      181 tctcgtcgat gaacccgcgc atgcgcgtgc gcgacatcat cggcgagccg ctgcgcaact
      241 tcggcgccga ttcggcgaag atcaaccagc gcgtgtccga gctgctcgag cgcgtgagcc
      301 tgcgcccgga cgcagcgacc cgctatccgc acgagttctc gggcggccag cgccagcgca
      361 tcgtggtcgc gcgcgcactc gcattgcaac cgaagctgat catctgcgac gagccggtgt
      421 ccgcgctcga cgtctcggtg caggcgcagg tgatcaacct gctgcgcgac ctgcagcgcg
      481 acatgaacct gtcctacgtg ttcatctcgc acgacctgtc ggtggtgcgc tacatcagcc
      541 accgcgtagc ggtgatgtac ctcggacaca tcgtcgaatc cggcccgcgc gaagcggtgt
      601 tccagtcgcc gacccatccg tacacgcagg ccctgctgtc tgccgtgccg gaagtcggca
      661 agcgcaacaa gaccatccag atcctcaagg gcgaaatccc gagcccgatg tcgccgccgt
      721 ccggctgcac cttccgcacc cgctgcccgc gggcgcaggc cctgtgtgcc gagcgcgcgc
      781 cggaaatgaa aaccataggc gaaggccatc aggccgcctg ccacttcgta taactttgga
      841 gcgaccatgt cggacatcac ccgttacccc agcaccctgc cctttccgtt ctcgcgcgcc
      901 gtacgcgccg gcggcttcct gttcctctcc ggccagattc cgatggatgc cgaaggcaag
      961 gtcgtgcagg gcgacatcac cgagcagacg cgcgcggtga tggagcgcat cggcgaatcg
     1021 ctgcaggcct gcggcgcaag ctatgcggat gtggtcaagg tcacggtctg gctgtcggat
     1081 ttctcgcagt tcgccgcctt caacgaggtc tacaagacct acttcaagga cggcttcccg
     1141 gtacgctcgg cggttgaagc caagctggcg ttcaacgtcg gcgtggaagt ggaagtgcag
     1201 gcctgggtcg gcgaccgatg agcgcccaac ccttcgacct cgtgctggtc ggcggcaata
     1261 tcatcgacgg caccaatcgt ccggccttca aggcggacat cggcatcatc ggcgaacgca
     1321 ttgtggcgat cggcaatctg gccgccagcc cggcccgcgc gcggcgcaac atcaccggcc
     1381 gcacggttgc gcccggtttc atcgattcgc acacgcacga cgacacgctg ctgctgcgca
     1441 agcgcgatat ggcgcccaag gtctcgcaag gcgtgaccac ggtggtcacc ggcaactgcg
     1501 gcatcagcct cgcgccgctc aacctggatg gcggcgtgcc gccggcgccg atgaccctgc
     1561 tcgatgcggg cggcgacttc aggttcgggt ctttctccgc ctacatggac gcgctgaagg
     1621 ccacgccgcc ggcggtcaat gccgcctgca tggtcggcca ttcgacgctg cgcgcggccg
     1681 tgatgggcga cctggaccgc cccgctaccg agggcgagac cgaatcgatg cgcgctctcg
     1741 cggacgaggc gatgcgcgcc ggcgcgatcg gcctgtccac cggcaccttc tacccgccag
     1801 ccgcgcatgc gaccaccgag gaaatcatcg aagtctgccg tccggttgcc gagcagggcg
     1861 gcatctacgc gacgcacatg cgcgacgaag gcgacaagat cgtcgaggcg ctggaagaaa
     1921 ccttcctgat cggccgctcg ctgaacgtgc cggtcgtgat ctcgcaccac aaggtgatgg
     1981 gcgcgaacaa tttcggccgc tcgaaggaaa cgctcgccat catcgagaag gccatggccg
     2041 agcaggacat ctcgctcgac gcctatccgt acatcgccgg ctcgaccatg ctgaagaagg
     2101 accgtacgct gctggcggcc aagaccatca tcacctggtg caagcccttc cccgaactgg
     2161 ccgggcgcga tctcgacgaa gtcgcagccg aacgcggcac caccaagtgg gacatcatcg
     2221 attcgctgca gccggccggc gcgatctact tcatgatgga cgaggccgat gtgcagcgaa
     2281 tcctcgcctt tcccgagacc atggtcggct ccgacggcct gccgcacgac gagcgtccgc
     2341 acccccgcct gtggggtacc ttcccgcgcg tgctcggcca ctatgcgcgt gatgtgaaac
     2401 tgttcccgtt ggagacggcg gtgtggaaga tgagcggctt gactgctgcc aagttcggcc
     2461 tgaaggaccg cggcgtgatc cgtgagaacg cctttgccga cctggtcgtg ttcgacccgg
     2521 ccacgattgc cgatgcggcg gacttcgatc atccgacgcg catctcgccc ggcatcgacg
     2581 tggtattcgt caacgggcaa accatctggg agaatggcgc cgccaccggc aactacccgg
     2641 gccaggtctt gaaaagggag gcggcatgag ccagcccatg agccaacgct tggtccacct
     2701 tgccgtagcc cagatgggtc cggtccagcg cagccacgcg cgcgcggacg ccgtcgaacg
     2761 cctggttgcg atgctgcagg aagccgcgcg ccgcggcgcg caatgggtcg tcttccccga
     2821 actcgcgctg accaccttct tcccgcgctg ggacctgcag gaccaggccg agatcgacgc
     2881 gttctacgaa accgagatgc cgaacgcggc caccgcgccg ctgttcgccg ccgcacgcga
     2941 acggaagatc ggcttctacc tcggctatgc ggaactggtg aacggccgtc atttcaacac
     3001 cagcatcctg gtcggtcagg acggcaacat catcggcaag taccgcaaga tccacatccc
     3061 cggtcatgcc agcagccaga ccggcggcgc cccgatccag cacctggaaa aaaagtactt
     3121 cgaggtgggc gacctgggct tccccgtgtg gcaaacgcgc gacgcaaaga tcggcatgtg
     3181 catctgcaac gaccggcgct ggcccgaaac ctggcgcagc atgggcctgc agggcatcga
     3241 gatcggcatg gtcggctaca acacgccgtc ggccttcgtg cagtgggatg agccgatcca
     3301 cctgcgcatg caccaccacc tgctctcgct gcaggcggcg gcctaccaga acgcctgctg
     3361 gatcgccgcg tcgggcaaag ccggcgtcga agaaggtagt cacatgatcg ggggttcgtg
     3421 tatcgttgcc cccacgggtg aagtgatggt caagacccag ggcgaaggtg acgaggtgat
     3481 cagtttcgaa tgcgatctct cgatcggcga ccggtaccgg gccaatatct tcaacttcgc
     3541 cgcgcaccgc aggaccgagc actacggcct gctggtggaa agaacaggag ccggcgcccc
     3601 attgggcacg acgccggagt gagcagcctc acagagtcaa acatgactat gcttttgcgc
     3661 ggcacgcccc acccgggcgt tgccaccgac cttgccgaac gccacactga gtccgccggc
     3721 acacgcaccc ttacccgtcc cgccatcgcg tcgaaagagg aagtcggccg ccgactgcgc
     3781 gcactgcgca agtcgagcaa gctgacactg aagatgttgt ccgaggccag cggcattcca
     3841 ctgtcgacga tctccaagat ggaactcggg cagatcgcag tcagctacga aaaattcagc
     3901 gcgattgcgc acgcgctcaa tgtcgacccg tcgagcctgt tcgatggcag cagcgcaagt
     3961 accgaagcgc gcccaaccgt ggtcaagtca tcggccgcgt ccaccgccga gtatcggtcg
     4021 gaaacctaca tccaccgcat gctcgccggc gacttcccgc acaagaagat gacgccgatg
     4081 caggcgcaac tgcttgcgcg tgaccgcagc gaattcgcgg acttcatccg ccaccccggg
     4141 gaggagtttg tcatggtgtt gtccggcacg gtgcgcatcg agttcgagac cggggagtcg
     4201 attaccttga agcggcacga gtctgcctat ttcaacagca gcatcggaca tatctatctt
     4261 tcggtgggac gcggaaacgc cgaggtcgtg gcggtgtgca cggacaagta aggcggcgcg
     4321 aacgaggacc gcgcatgcgc cgcacccgac ccgaggccgc gtgcggcgct gaaccatttg
     4381 tgcgctatcg actatcccgc cgcgcctgct gacgccgttg aaccgagccg acgcgccctc
     4441 gctcacgcgg attgcgcgca aattccggtc ccttggggtt caccggggat ttggtgcggg
     4501 tgcgccgggt actagcgcca tcggcctttg ctggcgcctt cgccgagcgc gatggtgcag
     4561 cagtggtcgc atcgaccgcc tcatccttga tcttggccgc cgccttggcc accgtacgca
     4621 ccaggcgttg cagagtggga acggcggcac gctccttgga cgccggcttg ttggcagcac
     4681 gcgtcgcact cttctttttc ggagctgcgc cggacgcctt ggcggccttg gccggtttgt
     4741 ctgccttggc cgcttttgcc gtcgatgccg ccttgatggc cgatttcggc ttggcgcctg
     4801 ccttcttcac agcggtgccg gaccgcgccg cgctcgacct gccggccttg gcggcggcgg
     4861 tgcggctctc tggcgctgcg ctgc
//
LOCUS       DATLGF010000247         2558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_89325,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000247 DATLGF010000000
VERSION     DATLGF010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89325"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..446
                     /locus_tag="VM406_07480"
     CDS             12..446
                     /locus_tag="VM406_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HVL75842.1"
                     /translation="MRNRAGQRGAAIIEFALILPLLLLITVITTDFGRAIYQYHTLTK
                     SVRDAARYLSAQTPGEGAAQARNLVVYGNPQGSGTPLAPGLTLAHVPTPTWQSTGTNP
                     VINSVTIGVSGYTFRSMFASVFGVNFGNFTYNDIRATMRAPS"
     gene            443..874
                     /locus_tag="VM406_07485"
     CDS             443..874
                     /locus_tag="VM406_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HVL75843.1"
                     /translation="MRTKQSGATTVEFALSLLIFLTFLLGMTDFSRMLFMWNAASEAA
                     RMGARYAVVCSDGSGNAAVLARMQRLLPQIQSVDLTWTPSGCNVANCEGVTVTITNLN
                     YQWISPIAGMSTLGAIPMPGFSTYLPREVMRQDPNSEAICS"
     gene            890..2041
                     /locus_tag="VM406_07490"
     CDS             890..2041
                     /locus_tag="VM406_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVL75844.1"
                     /translation="MKIVLTSSYPSRLRELASLIEAGPHALIAQDGAGARLAAAIEQH
                     QPELLVVEAGGREEEALRDIEAINLRHPGLAVILLCSDATPELLVQAMRSGVREVFAY
                     PVQAAVLDAGIARVAAKLATGSTRASAQVLAFLPCKGGSGATFLATNLGYQLAKRKSV
                     LLVDLNLQMGDALTFVHDEPPASTLAHVAREIRRLDATLLAASTVRVAPNYSILAAPE
                     DPSQAFEIKPEHVGAVLDVALGQYDFVLLDLGRSIDPVSMKALDYAHRIYPVFQAGLP
                     YLRNARKMLAALAALDYRADRIEPIVNRYDRRGEIGLDAITRLMPGLRLHTVPNAYKQ
                     VSASINQGTPLVETARTSTVSRRLTEFTHQISPSHDEGRGVFGRLFGRA"
     gene            2089..>2558
                     /locus_tag="VM406_07495"
     CDS             2089..>2558
                     /locus_tag="VM406_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HVL75845.1"
                     /translation="MLSTAHAERAAPSPAVAGGDAYKALKGRIHLKLLEKLDLSALET
                     LAPEKLRQEIAAQIERLLLDEQAPVNDIERRLLIRDIQHEMLGFGPIEVLMADPSVSD
                     ILVNSHDQVYVERRGKLELTNVTFTDERHLMRIIDKIVSLVGRRIDESSPMVDAR"
BASE COUNT          494 a          808 c          796 g          460 t
ORIGIN      
        1 gaggggcgca gatgcggaac cgagccggac aaagaggcgc tgcgatcatc gaattcgctc
       61 tgatcctgcc gcttcttctg ctgattacgg tgatcacgac cgacttcggg cgcgcaatat
      121 atcagtacca cacgctgacc aagtcagtgc gtgatgcggc ccgctacctg tcggcgcaaa
      181 cacctggcga gggtgcggca caggcgcgca acctggtcgt ctacggcaac cctcagggaa
      241 gcggaacgcc gcttgcccct gggctcacgc ttgcgcatgt gccgacacca acatggcaga
      301 gcacagggac caatccggtc atcaactccg tgaccatcgg cgtgagcggc tacaccttcc
      361 ggtcgatgtt cgcaagcgta ttcggtgtga acttcgggaa cttcacctac aacgacatcc
      421 gagccaccat gcgggccccc tcatgagaac gaaacaatcc ggcgccacta cggtcgagtt
      481 cgcgctatcg ctgctgatct tcctgacctt cctgctcggc atgaccgatt tcagccgcat
      541 gctcttcatg tggaacgctg cgagcgaagc agcgcgaatg ggtgcgcgct atgccgtcgt
      601 ctgcagcgac ggctcgggaa atgccgccgt gctcgcgcgg atgcagcgtc tgctgcccca
      661 gatccagagc gtggacctga catggacgcc gagcggatgc aatgtcgcca actgcgaggg
      721 cgtcaccgtc acgatcacca atctcaacta ccagtggatt tctccgattg ccgggatgtc
      781 gacgctgggc gcgattccga tgcccgggtt ctccacatac ttgccgcgcg aggtgatgcg
      841 gcaggatccg aatagcgaag ccatctgttc ctgattctgg aaaggactca tgaagatcgt
      901 tctgacatcg tcgtatccgt ccaggctgcg cgagcttgca agcctgatcg aagcaggacc
      961 gcacgcgctg atcgcgcagg acggagcagg tgccaggctg gccgcggcaa tcgaacagca
     1021 ccagcccgag ctgctcgtgg tcgaggcagg cggcagggaa gaggaggccc tgcgcgacat
     1081 cgaggcgatc aaccttcgcc atcccggcct ggcagtgatt ttgttgtgct ctgatgcaac
     1141 ccccgaactg cttgtccagg ccatgcggtc cggcgtgcgc gaggtgttcg cctacccggt
     1201 gcaggcggcg gtgctcgacg cgggaattgc acgggtcgcc gcaaagctgg caacgggatc
     1261 cacgcgcgca agcgcccagg tgctggcttt tttgccgtgc aagggtggca gcggtgccac
     1321 cttccttgca accaacctcg ggtatcaact cgccaagcgc aagagcgtgc tgctggtcga
     1381 cctcaatctg cagatgggtg acgcactgac tttcgtgcac gacgaacctc ccgcgtcgac
     1441 cctggctcac gtggcgcgcg agatccggcg gctcgacgcc acgctcctgg cagccagtac
     1501 ggttcgcgtt gcgccgaact acagcattct cgcggcgccc gaagatccgt cgcaggcgtt
     1561 cgaaatcaag cccgagcacg tgggcgcggt gctggatgtc gcgctcggtc agtacgattt
     1621 cgtgctgctc gaccttgggc gcagcatcga tccggtgagc atgaaggcgc tcgactacgc
     1681 acaccgtatc tatccggtct tccaggccgg gctgccgtac ctgcgcaatg cgcgcaagat
     1741 gctcgccgcc cttgcagccc tggactaccg ggcggacagg atcgaaccga tcgtcaaccg
     1801 ctacgacagg cgcggcgaga tcggtctgga cgccatcacg cgcctgatgc cgggcttgcg
     1861 cttgcatacc gtgcccaatg catacaagca ggtgagcgcg tcgatcaacc agggaactcc
     1921 gttggtggaa acggcgcgca cgagcactgt gtcgcgcaga ctcaccgagt tcacgcatca
     1981 gatcagtccg tcgcacgatg aaggccgggg cgtgttcggc cgcctgttcg gtcgtgcctg
     2041 acgaatgtgc cgccagtcca tggaggaaat gacatgtcgt tgcgcgaaat gctcagtaca
     2101 gctcatgccg aaagggctgc gccatcgccg gccgtcgccg gcggcgatgc ctacaaggca
     2161 ctgaaaggac ggattcacct caagctcctt gagaaactcg atctgagcgc gttggaaacg
     2221 cttgcgccgg aaaagcttcg gcaggagatc gccgcccaga tcgaacggct gctgctggac
     2281 gagcaggcgc cggtcaacga catcgagcgg cgcctcctga tccgcgacat ccagcacgag
     2341 atgctgggct ttggaccgat tgaagtcctg atggccgatc ccagcgtgtc cgacatcctc
     2401 gtgaattccc atgaccaggt gtatgtcgag cggcgcggca agctggagct gaccaatgtg
     2461 acctttaccg atgaacggca cctgatgcgc atcatcgaca agatcgtctc gctggtcggg
     2521 cgccgcatcg atgaatcgag tccgatggtc gacgcgcg
//
LOCUS       DATLGF010000248         3887 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_194851, whole genome shotgun sequence.
ACCESSION   DATLGF010000248 DATLGF010000000
VERSION     DATLGF010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194851"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1416)
                     /locus_tag="VM406_07500"
     CDS             complement(145..1416)
                     /locus_tag="VM406_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HVL75846.1"
                     /translation="MDTVPIWAQALALVLLICFSSFFSISETALMALNRHRVKHLARR
                     GNRLARITLFLLENTEKVLALILVSNTVINALITALVTAIAIGMFGNNEAVITGATAL
                     VAFLLIVFSEITPKVIGATYPERIALPASFLLKPLMAVATPAIWFVNLFVRALLRMLG
                     IKTGASARDHRVSPEELRSIVLEAGSFIPQKHRSILLNMFDLERVSVEDIMTPRSQIE
                     AINLSAPIDEIMHQLATCYHNKLPVYEGEINHVIGILHIRTVLALLTQEGEFTADRIR
                     GQLSAPYYIPQDTDVFRQLQYFQENHERLGIVVDEYGEVQGLVTLEDIIEEMIGEFTT
                     SLHGAGRSDAFSWNENGECLLEGSTSLRDINKHLHLSFPLDGPKTVNGLLLEVLRDIP
                     EAQISLKLGGCIVEIVQVQNQAIRSVKLRRL"
     gene            complement(1509..1585)
                     /locus_tag="VM406_07505"
     tRNA            complement(1509..1585)
                     /locus_tag="VM406_07505"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1549..1551),aa:Pro,seq:cgg)
     gene            complement(1626..2630)
                     /gene="ispB"
                     /locus_tag="VM406_07510"
     CDS             complement(1626..2630)
                     /gene="ispB"
                     /locus_tag="VM406_07510"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HVL75847.1"
                     /translation="MFPSHILSAAPTPASHTIVTPIAADMKDVDAVIRAKLQSDVALI
                     NQIGEYIIGAGGKRIRPMLLLLTANAHGYRGTHHHTLAAVVEFIHTATLLHDDVVDES
                     SMRRGRETANAMFGNAASVLVGDFLYSRAFQMMVSVDSMRVMRILADATNVIAEGEVL
                     QLLNMHDPDVTEERYLQVIRSKTAKLFEAAAELGALVAGATDGDVEAAAEYGRSLGTA
                     FQLIDDVLDYSGNATAIGKNVGDDLREGKPTLPLIYLLEHGSPDQRELVRSCIEHGDE
                     ARFDEVLAAITESGALTYTRKHAQAAARRAEEAISNMPASAFKNSLLQLCAFAVERDH
                     "
     gene            2852..3163
                     /gene="rplU"
                     /locus_tag="VM406_07515"
     CDS             2852..3163
                     /gene="rplU"
                     /locus_tag="VM406_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HVL75848.1"
                     /translation="MYAVIKTGGKQYKVAAGEKLKVEQIPADIGSEITLDQVLAVGAG
                     DSIKFGAPLVDGAKVQATVVAHGRHDKVKIFKMRRRKHYQKRQGHRQNYTELQIVSIN
                     A"
     gene            3201..3458
                     /gene="rpmA"
                     /locus_tag="VM406_07520"
     CDS             3201..3458
                     /gene="rpmA"
                     /locus_tag="VM406_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HVL75849.1"
                     /translation="MAHKKGGGTTRNGRDSESKRLGVKVYGGQAINAGGIIIRQRGTR
                     VHAGENVGMGKDHTLFALVDGKVQFTTKGALKRHTVTVVPA"
     gene            3546..>3887
                     /gene="obgE"
                     /locus_tag="VM406_07525"
                     /gene_synonym="cgtA"
                     /gene_synonym="obg"
                     /gene_synonym="yhbZ"
     CDS             3546..>3887
                     /gene="obgE"
                     /locus_tag="VM406_07525"
                     /gene_synonym="cgtA"
                     /gene_synonym="obg"
                     /gene_synonym="yhbZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395159.1"
                     /note="ObgE; essential GTPase; exhibits high exchange rate
                     for GTP/GDP; associates with 50S ribosomal subunit;
                     involved in regulation of chromosomal replication; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HVL75850.1"
                     /translation="MKFIDEARIEVIAGDGGNGVVSFCREKFRPFGGPDGGDGGKGGS
                     IWAVADRNINTLVDFRYSKLHKARNGEHGRGSDCYGKGADDIVLRMPVGTLVSDLDTG
                     ELIADLTEHEQT"
BASE COUNT          782 a         1136 c         1255 g          714 t
ORIGIN      
        1 cctgtgcacg atatgccacg cgtatgcggg ccgggcagac ttggaggtcc tcgcaaacgg
       61 cgatcgcagg ccgcgccggc gcaggcaata cgtgtggtgg cgcaaggagg cgcaacgaag
      121 caggcgacta tcggtcaagg ctgcttaaag cctgcgcagc ttgacgctcc tgatcgcctg
      181 gttctgtact tggacgatct cgacgatgca gccgccgagt ttcaggctga tctgggcctc
      241 ggggatgtcg cgcaggacct cgagcagcag gccattgacc gtcttcggcc cgtccagcgg
      301 gaaggacagg tgcagatgct tgttgatgtc gcgcaggctg gtgctgccct cgaggaggca
      361 ctcgccgttt tcgttccacg agaatgcgtc cgagcgaccg gcgccgtgca gcgaggtggt
      421 gaactcgccg atcatttcct cgatgatgtc ttccagcgtg accagcccct ggacctcgcc
      481 atactcgtcg acgacgatgc cgaggcgctc gtgattctcc tggaaatatt ggagctggcg
      541 gaacacgtcg gtgtcctgcg ggatgtaata cggcgcgctc aactgccccc ggatgcggtc
      601 ggccgtgaac tcgccttcct gggtcagcag ggcaagcacg gtgcgaatgt gcagaatgcc
      661 gatcacatga ttgatctcgc cctcgtagac cggcagcttg ttgtgatagc aggttgccag
      721 ctggtgcatg atctcgtcga tcggcgcaga gagattgatg gcctcgatct gcgagcgcgg
      781 cgtcatgatg tcctcgaccg acacgcgctc gagatcgaac atgttgagca ggatgctccg
      841 gtgcttctgc gggatgaagc tgcccgcctc cagcacgatc gagcgcaatt cctcgggcga
      901 gacgcgatgg tcgcgcgcgc tggcgccggt cttgatgccc agcatgcgca gcagggcgcg
      961 cacgaacagg ttgacgaacc agatggccgg cgtcgccacc gccatcagcg gcttgagcag
     1021 gaatgatgcc ggaagggcga tgcgctcggg ataggtcgcc ccgatgacct tcggagtaat
     1081 ctcggaaaac acgatgagca ggaaggccac cagcgcggtg gcgccggtga tcaccgcctc
     1141 attgttgccg aacatgccga ttgcgatcgc cgtcaccagt gccgtgatca acgcgttgat
     1201 caccgtgttc gacaccagga tcagggcgag gaccttctcg gtgttctcaa gcaggaagag
     1261 ggtgatccgc gcaaggcggt tgccgcgccg tgcgagatgc ttgacccgat gccggttcag
     1321 cgccatcagc gccgtctcgg aaatcgagaa gaaggaggaa aaacagatca ggagaacgag
     1381 cgcaagagct tgcgcccata tgggtacggt atccaaatgt caccgcgtgg aaatgtgcat
     1441 cgcttcttgc agccgaggcg gctgcttgtt acgcaagtcg gcttgcggcg tctcgtgcat
     1501 caggaaactg gtcggggtga gaggattcga acctccggcc tctacgtccc gaacgtagcg
     1561 ctctaccagg ctaagctaca ccccgaatca acggcttgcg acccaggcgg acagtgtata
     1621 tccgctcagt gatcacgttc aaccgcgaaa gcgcataatt gtagcaggga attcttgaag
     1681 gcgctagcgg gcatattcga aatcgcctcc tccgcacgcc gggcggcggc ctgcgcatgc
     1741 ttgcgcgtgt acgtgagcgc gcccgattcc gtgatcgcag cgagaacttc gtcgaagcga
     1801 gcttcgtcgc catgctcgat gcagctgcgc acgagttcgc gctggtcagg gctgccgtgt
     1861 tcgagcagat agatcagcgg cagcgtcggc ttgccttcgc gcagatcatc acccacattc
     1921 ttgccgatgg ccgtcgcgtt gcccgaatag tcgagtacgt cgtcaatcag ctggaacgcc
     1981 gtgccgagtg aacgaccata ctcggcggcg gcctcgacgt cgccatccgt cgcgccggca
     2041 accaatgcgc cgagctcggc ggcggcctcg aacagcttgg cggtcttgga gcggatgacc
     2101 tgcaggtagc gctcctcggt gacgtcggga tcgtgcatgt tgagcaattg cagcacttcc
     2161 ccttcggcga tcacgttggt tgcatccgcg agaatgcgca tgacgcgcat gctgtcgacc
     2221 gaaaccatca tctggaaggc gcgcgaatag aggaaatcgc ctaccagcac cgatgccgcg
     2281 ttgccgaaca tcgcgttcgc cgtttcccgg ccgcgccgca tcgaggattc gtcgaccacg
     2341 tcatcatgga gcagcgtcgc ggtatggatg aattcaacca ctgcggcgag cgtgtgatga
     2401 tgggtgccgc ggtagccgtg cgcgttggcg gtcagcagaa ggagcatcgg gcggatgcgt
     2461 tttccgccgg cgccaatgat gtattccccg atctggttga tgagcgcgac gtccgactgc
     2521 aacttcgcgc ggatgaccgc gtcgacgtcc ttcatgtcgg cggcgatcgg agtgacgatg
     2581 gtatggctgg ctggagtcgg agcggcggac aagatgtgac tcgggaacat gtgcatgaaa
     2641 ggcacgatta tacgatggcg ccttggtgtg gggccgcgct tgggaacggg gccgtactgt
     2701 gcccggaggc atgcggacct cgaatacatg ctttgaccgc gtagctaagc ctatgtataa
     2761 tcaggggttt tcctgtttcc gcagctcggt gccgcggcgg gagaaaaggc ccggtaccgg
     2821 gcttactttt tcaacatttg atgaggttca catgtacgcg gtcataaaaa ccggcggcaa
     2881 acagtacaag gttgctgctg gcgaaaaact caaagtagaa cagataccgg ctgacattgg
     2941 atccgaaatc actctcgatc aagtgctcgc agtgggcgct ggcgacagca tcaagttcgg
     3001 tgctccgctg gtcgatggtg ccaaggtcca ggctacggta gtggcgcacg gtcgccacga
     3061 caaggtgaag attttcaaga tgcgccgccg caagcactat cagaagcgtc agggccatcg
     3121 ccaaaactac accgaactgc aaatcgtctc gatcaacgcg taatcgcgga tcgggctaac
     3181 cggaaaccaa ggagtctgaa atggcacaca aaaagggcgg cggcactacg cgcaacggcc
     3241 gcgattccga gtcgaaacga ctcggcgtga aggtctacgg cggtcaggcc atcaatgccg
     3301 gcggcatcat catccgtcag cgcggcaccc gcgtgcacgc aggcgagaac gtcggcatgg
     3361 gcaaggacca caccctgttc gcgctcgtcg acggcaaggt gcagttcacc accaagggcg
     3421 cactgaagcg ccacactgtc acggtcgtgc cggcgtaagc agcggcgacc tgagcacaaa
     3481 ggcgcaagcc ttgcatcgaa gggctctgcc tccggtaggg cctttttact ttctggcggc
     3541 aaaacatgaa gtttatcgac gaagcaagaa tcgaagtcat tgccggcgac ggcggcaacg
     3601 gcgtcgtgtc gttttgccgc gagaagttcc gcccgttcgg cggaccggac ggcggcgacg
     3661 gtggcaaggg cggcagcatc tgggctgtgg ccgaccgcaa tatcaacacc ctggtcgact
     3721 tccgttattc caagctgcac aaggccagga atggcgagca cggccgcggg tccgactgct
     3781 acggcaaggg cgccgacgac atcgtgctgc gcatgccggt cgggaccctg gtgtcggatc
     3841 tggacaccgg cgagctcatc gccgacctga ccgaacacga gcagacg
//
LOCUS       DATLGF010000249         3667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_26734,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000249 DATLGF010000000
VERSION     DATLGF010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26734"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VM406_07530"
     CDS             <1..404
                     /locus_tag="VM406_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVL75851.1"
                     /translation="PVQYTASDGRFRLGVLTDAGHGTAHITDALGGCDALLLECNHDP
                     AMLAASNYPPSLKARIGGPYGHLSNAAAAQVLAALDRSRLSMVVAAHLSQQNNTPELA
                     HAAVHQVVGETCEIRVACQHDGFGWIGAGAS"
     gene            complement(472..714)
                     /locus_tag="VM406_07535"
     CDS             complement(472..714)
                     /locus_tag="VM406_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75852.1"
                     /translation="MNKNLLIASLLALALTACGQKEEAAAPAQEPAPVAAAPAEEAAA
                     PAEEAQAEGEAKAEGEAQPTDAQAAAEQPAGEAPKQ"
     gene            complement(1005..2126)
                     /locus_tag="VM406_07540"
     CDS             complement(1005..2126)
                     /locus_tag="VM406_07540"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVL75853.1"
                     /translation="MKKYNVLGAMSPQKFLREYWHKRPLLIRGAIAGFKPVLSREALF
                     GMAADPAVESRLITHQDERWTVEHGPFKRLPSTRKPHWTALIQGVNLHDDKAAALLEQ
                     FRFIPDARLDDLMISYATDGGGVGPHFDSYDVFLLQALGRRRWRISAQDDLTLVEGLP
                     LKILKHFEAEEEYVLEPGDMLYLPPHVAHDGIAEGECMTYSIGFRAPSYQELGENFLA
                     FLADGVELQGRYADPDLRFSRRPAQLQAEMLDRIATEIRKLAFDDEDIAIFVGEYLSE
                     PKPAVVFRPPTRPLTRTRFLEAARRRGVRLARQTRMLYRGRHLFINGESFIVGSKDRH
                     VLSSLADARVLDAQELQAVSHDVEDALHHWYEDGWVRLR"
     gene            2237..2767
                     /locus_tag="VM406_07545"
     CDS             2237..2767
                     /locus_tag="VM406_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HVL75854.1"
                     /translation="MKIAKNTVVTVHYELSDAQGNLIEEGQEPMVYLHGGYENTLPKI
                     EEALEGKEPGYATTIQVEPEDAFGEYDPSLIKIEPRNRLPDPVEVGMQFEGTPEGGEG
                     DETVIFTVTDIAEDKVVLDGNHPLAGIALRFTLNVADVRAASAEEVSHGHVHGEHGHG
                     HDDDEDGDHYRSRPVH"
     gene            complement(2761..>3667)
                     /locus_tag="VM406_07550"
     CDS             complement(2761..>3667)
                     /locus_tag="VM406_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75855.1"
                     /translation="SCYDPMKRALLLLAAVLVAPAFAQPVGDLRYNFAVIGSRAYADD
                     VRLRDTLTQLDGANLAFVVAQGIKPANAPCNDTSYLRRREELDASKHGLVISVAATDW
                     ASCGQADGTTTALARLTRVRELFFPDEFSFGATRIPLVRQSTEAKFQNFVENARWEIG
                     SVMFATINLPANNNNFVTAAGRNGEFEDRLIANREWLRRVFTTATLKNASAVVLFADA
                     NPMAKPGDAPRDGFAETRRHLQMLASRFKGRVLIVHAQAEPEAPAVIQWRGNLGTLGV
                     ARGPVRLRVDPALPEVIMPRNPKVQ"
BASE COUNT          683 a         1113 c         1195 g          676 t
ORIGIN      
        1 aacccgtgca atacaccgcc tcggatggac gcttccggct cggcgtgctg accgacgcgg
       61 ggcacgggac ggcccacata accgacgcgc ttggcggctg cgacgcgctg ctgctggaat
      121 gcaaccacga cccggcgatg ctcgccgcat ccaactatcc gccttctctc aaggcccgta
      181 tcggcggacc gtacggacac ctgtcgaacg cggcggcggc gcaggttctc gccgcgctgg
      241 acaggtcgcg tctttcgatg gtcgtcgccg cgcatctgag ccagcagaac aatacgcccg
      301 aacttgcgca tgcagcggtg catcaggtgg taggcgaaac ctgcgagatc cgtgtcgcgt
      361 gccagcatga tggatttgga tggatcggtg cgggcgccag ctgaatcgct gcgttgccgt
      421 gagcagaaaa aaaaaccggc cgatcggccg gtttttttgt tgaagcagtg attactgctt
      481 cggggcttcg ccagccggct gctcggcagc agcttgtgcg tccgtcggct gagcttcgcc
      541 ttcagccttg gcttcgcctt cggcctgagc ttcttcagcc ggagcagcag cttcttccgc
      601 cggcgcggca gccaccggag cgggttcttg tgccggagca gcggcttctt ctttctggcc
      661 gcaagcggtc agggcgaggg ccagcaggga tgcgatcaac aggttcttgt tcatgatttg
      721 ccttatggga tttcaacagg attgaagcga cgccattacc agtggcgccg ccggtttcag
      781 gtcgtttcct ggcggtgttg cagcgccgca ctcgtgaaac ttcctagacg gaaattatag
      841 ccagatttga attttgcagc gcaacgattt atgttctcgt tgcacttttt cggcatgcgt
      901 gacgggacac cttgagagat ggcgtggcct acggacctca ggcagaaaga gcagcttgcg
      961 cggtcggcca ccacgacccc acaggagatc ctttccagag gttcctagcg caagcgcacc
     1021 catccgtctt cataccaatg atgcagtgca tcctccacgt cgtgagacac cgcctgcagt
     1081 tcctgcgcgt cgagcacacg ggcgtcggcg agtgaagaca ggacatgcct gtctttcgag
     1141 ccgacgataa agctctcgcc attgatgaag agatggcgtc cgcggtacag catcctggtc
     1201 tggcgtgcca gccgcacgcc gcggcggcgc gcggcctcga ggaagcgcgt gcgtgtcagc
     1261 ggccgcgttg gcggcctgaa aacgacggcg ggcttcggct cggacaggta ttcgccgacg
     1321 aatattgcga tgtcttcatc atcgaacgcg agcttgcgga tctcggtcgc gatccggtcc
     1381 agcatttccg cctgcaattg cgccggtctt cgcgagaaac gcaggtctgg atcggcatag
     1441 cgcccttgta actccacacc atcggcaaga aatgccagga agttctcgcc gagctcctgg
     1501 tacgacgggg cgcgaaaacc gatcgaatat gtcatgcact cgccctcggc tatgccgtcg
     1561 tgggcgacgt gcggcggcaa gtagagcatg tcaccgggct cgagcacgta ttcctcctcc
     1621 gcctcgaagt gtttgaggat cttcagcggc aagccttcga ccagcgtgag gtcgtcctgc
     1681 gcgctgatgc gccagcgccg ccttccgagg gcctgcagca ggaatacgtc gtaggaatcg
     1741 aagtgcggac cgaccccgcc gccatccgtt gcatagctga tcatcaggtc gtccaaccgc
     1801 gcgtccggga tgaagcggaa ctgctccaac agggcggcgg ccttgtcgtc gtgcaggttc
     1861 acaccttgga tgagggcggt ccaatgcggc ttgcgcgtgg agggcaggcg tttgaaggga
     1921 ccgtgctcca ccgtccagcg ctcgtcctga tgcgtgatca ggcgcgattc gacggccgga
     1981 tccgcggcca tgccgaacaa ggcctcgcgc gagaggacgg gcttgaaccc ggcaatggcg
     2041 ccgcggatca gcagggggcg tttgtgccag tactcgcgaa ggaacttttg cggggacatc
     2101 gcgcccagaa cattatattt tttcattgcg gggtcggtag caggcattgc tgataagctt
     2161 cggggcttgc agcggcgcgg cccgacgcaa gtggcacagt ccgcgctgct acccgataca
     2221 tggaaaggaa gcgtcgatga aaatcgccaa aaacacggta gtgaccgtgc actatgagtt
     2281 gtccgacgcg cagggcaatc tgatcgagga aggccaggaa ccgatggtgt atttgcacgg
     2341 cggctacgag aacacgctgc cgaaaatcga ggaggcactc gaaggcaagg aacccggtta
     2401 cgccacgacg atccaggtcg agcccgaaga cgcgtttggc gaatacgatc cgagcctgat
     2461 caagatcgag ccgcgcaacc gccttcccga cccggtggaa gtcggcatgc agttcgaagg
     2521 cacgccggaa ggcggagagg gcgatgaaac ggtgatcttt accgtgaccg acatcgccga
     2581 agacaaggta gtgctggacg ggaatcaccc gctggccggc atcgcgctgc gctttacgct
     2641 gaatgtggcc gatgtgcgcg ccgcgagtgc ggaagaagtg tcccacggcc atgttcacgg
     2701 cgagcacggt cacggccacg atgacgacga ggacggcgac cattaccgca gccggcccgt
     2761 tcactgaacc ttggggttgc gcggcatgat cacctccggg agggcgggat cgacgcgcag
     2821 gcggaccggt ccgcgcgcca cgccgagcgt gccgaggttg ccgcgccatt gaatcaccgc
     2881 aggtgcctcc ggttcggcct gcgcgtggac aatcagcacc cgtcccttga accgggacgc
     2941 gagcatctga aggtggcgcc gcgtctcggc aaatccgtcc cgcggagcgt cacccggttt
     3001 cgccatcgga ttggcatcgg caaacagcac gacggcgctc gcgttcttca gcgtcgccgt
     3061 ggtgaataca cgacgcagcc attcacggtt tgcgatcagg cggtcttcga attccccgtt
     3121 gcgtccggct gccgtcacga aattattgtt gttcgccggc agattgatgg tggcgaacat
     3181 aacactgccg atttcccagc gcgcgttctc tacgaagttc tggaacttcg cctcggtgga
     3241 ttgccgcacc agcgggatgc gggtggcgcc gaatgaaaac tcgtcgggga agaacagctc
     3301 gcgcacccgc gtcaggcgcg caagggcggt cgtcgtgcca tcggcctggc cgcagctcgc
     3361 ccagtccgtc gcggcgaccg atatcaccag cccatgcttg ctggcgtcga gttcttcgcg
     3421 ccggcgcaga tagctcgtgt cgttgcacgg cgcattggcc ggcttgatgc cctgcgcgac
     3481 gacgaaggcc agattggcgc cgtcgagctg ggtgagggtg tcgcgcagtc gtacgtcgtc
     3541 ggcataggcg cggctgccga tcaccgcgaa gttgtagcgc aggtctccca ccggctgggc
     3601 gaaagccggt gcgacgagaa cggcggcgag cagcaagagg gcgcgcttca tagggtcgta
     3661 gcaagat
//
LOCUS       DATLGF010000250         4456 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_149539, whole genome shotgun sequence.
ACCESSION   DATLGF010000250 DATLGF010000000
VERSION     DATLGF010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149539"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /gene="carB"
                     /locus_tag="VM406_07555"
     CDS             complement(<1..480)
                     /gene="carB"
                     /locus_tag="VM406_07555"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326830.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HVL75856.1"
                     /translation="MPKRSDIKSILIIGAGPIIIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPITWQVVERIIAKERPDAILPTMGGQTALNCALDL
                     HRNGVLTKYGCELIGATPEAIDKAEDRLKFKDAMTKIGLGSARSGIAHTLDEAWAVQR
                     "
     gene            complement(473..1639)
                     /gene="carA"
                     /locus_tag="VM406_07560"
     CDS             complement(473..1639)
                     /gene="carA"
                     /locus_tag="VM406_07560"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HVL75857.1"
                     /translation="MLPFFSGQSVPAILALADGSTFQGYSIGAPGHTIGEVVFNTAIT
                     GYQEILTDPSYSRQIVTLTYPHIGNVGVNCEDVEAEKIHAAGLIIKDLPAVASNFRSE
                     QSLSDYLKSQNVVGIAGIDTRKLTRILREKGAQNGAILAGEADVEKALALARSFPGLN
                     GMDLAKVVSTKEPYVWTQTEWKLGSGYGEQKNPKYHVVAFDYGVKRNILRMLAERGCK
                     ITVLPAQSTAAEALALNPDGIFLANGPGDPAPCDYAIAATKEMIERGIPTFGICLGHQ
                     LMGLAVGAKTLKMKFGHHGANHPVQDVQTGQVMITSQNHGFAVDPATLPANCRVTHVS
                     LFDGSLQGFELTDKPAFCFQGHPEASPGPTDVAPLFDRFIGLMNQAMMEKQKNA"
     gene            complement(1851..2258)
                     /locus_tag="VM406_07565"
     CDS             complement(1851..2258)
                     /locus_tag="VM406_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HVL75858.1"
                     /translation="MQTKPVLTLDDVKKIAAAAEAEARANNWAVTISIVDDGGNLLWL
                     QRLDGAAAISAHIAPAKAHTAALGQRETKVYEDMINNGRFSFLSAPQLQGMLEGGVPI
                     MVEGKCVGAVGVSGVKSTEDAQIAKAGIAALGL"
     gene            complement(2329..3462)
                     /locus_tag="VM406_07570"
     CDS             complement(2329..3462)
                     /locus_tag="VM406_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL75859.1"
                     /translation="MSQDSLSSIPAKPRVLIADDSRIVRTALIKRIQGLFEFREAIDG
                     EQAWETLLLDPAIRVVITDLSMPKLDGYELLARIRGSRLRRIRELPVVVVSGSDEQEE
                     RARARAAGATDLITKGIATPQLLSRLEVLSSLVVSQREFERGLEALVRDRAGSTLPLL
                     GEQAFMEAATQQLDGARANRLDLVVLEIGLGIRHQGLDGFAAVPPAAVLEAIGQLLRR
                     TVRQEDLVARTADARFSLVADSLGPDAARRVAQRICHAIAGASLAGCEQTTLIACCGL
                     AALSDFSGVPHAPDIRALRGDAHRRALLGLDHGLTGVVGLAEEQAFRRGESRLEAALE
                     PDLATLLRWAKEGRHAELTPHLARLSSELAPLVELLQQQVPLR"
     gene            complement(3561..4409)
                     /locus_tag="VM406_07575"
     CDS             complement(3561..4409)
                     /locus_tag="VM406_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVL75860.1"
                     /translation="MPAPPVVSPVQPAVAPRPVKIGLALGGGAARGFAHIGVIKALEA
                     QGIVPDIVVGTSAGSLVGALYAAGNNGFALHKLALEMDEVAISDWAVPLFARTAGVLK
                     GEALQNYVNRTLDNLPLERMKIPFGAVATDLRTGTPILFRRGNTGQAVRASSAVPGVF
                     QAVRIGENSYVDGGLVAPVPVRFAREMGADFVIAVNISAQPESQPSATTLDLLLQTFA
                     IMGQSINQYELRDADIVIQPRLGTMKGNDFPARNSAILAGEQAAAAMMGELRRKLNEK
                     RGLTAP"
BASE COUNT          779 a         1438 c         1439 g          800 t
ORIGIN      
        1 cctttgcacc gcccaggcct cgtccagcgt atgggcaatg ccggaacgcg cggagccgag
       61 accgatcttg gtcatcgcat ccttgaactt caggcggtct tcggccttgt cgatcgcttc
      121 gggcgtcgca ccgatcagtt cgcagccgta cttggtcaac acgccgttgc ggtgaaggtc
      181 gagcgcgcag ttgagcgcgg tctggccacc catcgtcggc aggatcgcgt ccggacgttc
      241 cttggcgatg atgcgctcga ccacctgcca ggtgatcggc tcgatgtagg tgacatcggc
      301 catctccggg tcggtcatga tggtcgccgg gttgctattg acgaggatga ccttgtagcc
      361 ctcttcgcgc agggccttgc aggcctgcgc gccggaatag tcgaactcgc aggcctggcc
      421 gatgatgatc gggccggcgc caatgatcag gatgcttttg atatctgaac gcttaggcat
      481 ttttctgttt ctccatcatc gcctggttca tgagaccaat gaagcgatcg aacaagggag
      541 cgacgtcggt cggtccgggc gaggcttccg ggtggccctg gaagcagaat gcgggtttgt
      601 cggtcagctc gaagccctgc agcgagccgt cgaacagcga gacgtgggtg acgcggcaat
      661 tcgccggaag cgtcgccgga tcgacggcga agccgtggtt ctgggaagtg atcatcacct
      721 ggccggtctg cacgtcctgt accggatggt tcgcgccgtg gtgcccgaac ttcatcttca
      781 gcgtcttcgc gccgacggcc aggcccatta actggtgacc caggcagatg ccgaaggtcg
      841 ggatgccgcg ctcgatcatt tccttcgttg cggcgatcgc gtaatcgcac ggcgccggat
      901 cgccgggacc gttggcaagg aagatgccgt ccggattgag cgcgagcgcc tctgccgcgg
      961 tcgactgggc cggcagcacg gtgatcttgc aaccgcgctc ggccagcatg cgcaggatgt
     1021 tgcgcttgac gccgtagtcg aacgcgacga cgtggtactt cggattcttc tgttcgccat
     1081 agcccgagcc cagcttccac tcggtctgag tccacacata gggttccttg gtcgagacga
     1141 ccttggccag gtccatgccg ttgagccccg ggaacgaacg cgccagcgcc agcgccttct
     1201 cgacgtcggc ctcgcccgcg aggatcgcgc cgttctgggc gcccttctcg cgcaggatgc
     1261 gcgtaagctt gcgcgtgtcg atgccggcga tgccgaccac gttctgggac ttcagataat
     1321 ccgacagcga ctgttcgctg cggaagttcg aggccaccgc cggcaggtcc ttgatgatca
     1381 ggcccgcggc atggatcttc tccgcctcga cgtcttcgca gttcacgccg acgttgccga
     1441 tatgcggata agtgagggtg acgatctggc ggctgtagct gggatcggtc aggatttcct
     1501 ggtagcccgt gatggccgtg ttgaacacga cttcaccgat cgtatgaccg ggggccccaa
     1561 tcgaataacc ctgaaatgtc gacccgtctg cgagcgcaag gatggccgga actgattggc
     1621 cagaaaaaaa tggcagcaag ggtaactcct gaggtggtta ccgccgctgc gccgcgccgc
     1681 aaggcccgcc gaataccgct gcgcgcgtcg ttcggagacc ttttcgggac gtgtgggaag
     1741 gggtatgcgc taacggcggg ctggatttgg ctaaaccgcg aaattatatc cgagattggc
     1801 caatccaggc aaacgcacag gcgttttaga gcccgccgtc agagagaaaa tcagaggccg
     1861 agcgccgcga tccctgcctt ggcgatttgc gcgtcttcgg tggacttcac gcccgatacg
     1921 ccgaccgcgc ctacgcactt gccttccacc atgatcggca cgccaccctc gagcatgccc
     1981 tgcagctgcg gtgcgctcag gaacgagaag cggccgttgt tgatcatgtc ttcgtagacc
     2041 ttggtctcgc gctggccgag cgcggcagtg tgcgccttcg ccggagcgat gtgggccgaa
     2101 atcgccgccg cgccgtccag ccgttgcagc cacagcaggt tcccgccgtc gtccacgatc
     2161 gaaatcgtga ccgcccagtt gttcgcgcga gcttcggctt cggccgcggc cgcgatcttc
     2221 ttgacgtcgt ccagggtcag tacgggcttg gtctgcatgt tctctcctgc gtgcaatagc
     2281 tgcgaaaaag gcaggattgt acgccagaca cgcctgtggc gcagactcct agcgaagtgg
     2341 cacctgttgc tgcaacagtt cgaccagcgg cgccagttcg gatgacagcc gtgccagatg
     2401 cggcgtcagc tcggcgtgcc gcccttcctt ggcccagcgc aaaagcgtgg ccagatcggg
     2461 ctccagcgcc gcttccagac ggctttcgcc gcgacgaaac gcctgctcct cggccagccc
     2521 gacgacacca gtgagcccgt ggtcgagccc cagcaaggcg cggcggtgcg catcgccgcg
     2581 caaggcccgg atatccggcg cgtgcggtac gccgctgaaa tcgctgaggg cagccagacc
     2641 gcagcaggcg atcagcgtcg tctgctcgca gccggcgagg ctggcgccgg cgattgcatg
     2701 gcagatgcgc tgggcgacgc gacgtgcggc gtccggaccc agactatcgg cgacaagact
     2761 gaaccgcgca tcagccgtac gggcgacgag atcctcctgt cgcacggtac ggcggagcaa
     2821 ctggccaatc gcctcgagca cggccgccgg ggggactgcg gcaaaaccat cgaggccctg
     2881 gtggcgtatg ccgaggccga tttcgagcac gacgagatca agacgatttg cgcgggcgcc
     2941 gtccagctgc tgcgtggccg cctccatgaa cgcttgctcg cccagcaacg gcagtgtgct
     3001 gcccgcccgg tcgcgtacca aggcctcgag gccgcgttcg aattctcgct gactgacaac
     3061 caggctggag agcacttcca ggcgcgagag cagctgcggt gtggcaatgc ccttggtgat
     3121 caggtcagtt gcgccagccg cccgcgcccg cgctctctcc tcctgctcgt cgctgccgga
     3181 aacaaccacg acgggaagtt cacggatgcg tcgcagacgg gagccgcgaa tccgcgcaag
     3241 taattcatag ccatcgagct tcggcatcga caggtccgtg atcacgacac ggatggccgg
     3301 atcgagcagc aacgtttccc aggcctgctc accgtcgatc gcttcgcgaa attcgaacag
     3361 gccctgaata cgcttgatca gggcggtgcg cacgatccgg gagtcgtccg cgatgaggac
     3421 gcgcggcttg gccgggatgc tgctcaggct gtcttggctc atgcggcagg aattcgttgt
     3481 cttgcccgca agcatacccc gcaatgcggc gtttggcagc aacaccctca gtcgcgccgg
     3541 gtcgccgcta agagtgccgc tcagggggca gtcagcccgc gtttttcgtt cagcttgcgc
     3601 cgcaactcgc ccatcatggc cgcagccgcc tgttcgccag cgaggatcgc gctattgcga
     3661 gcggggaagt cgttaccctt catcgttccc aggcgcggct ggatcacgat atcggcatcg
     3721 cgcagctcat attgattgat gctctgtccc atgatcgcga aggtctgcag cagcaggtcc
     3781 agcgttgtgg ccgacggctg cgattccggc tgcgcggaaa tattcacggc aatgacgaaa
     3841 tccgcgccca tctcgcgcgc gaagcgcacc ggcaccgggg cgaccaggcc gccgtcgaca
     3901 taactgttct cgccgatgcg caccgcctgg aacacccccg gcaccgccga tgacgcgcgc
     3961 accgcctgcc cagtattgcc gcggcggaac aggatcggtg tgccggtccg caagtcggtg
     4021 gcaaccgcgc cgaacgggat cttcatccgc tccagcggca ggttgtccaa ggtccggtta
     4081 acgtagttct gcaaggcctc gcccttcaag acaccggcag ttcgcgcgaa cagcggcacg
     4141 gcccagtccg agatcgccac ctcatccatc tccagcgcca gcttgtgcag cgcaaagccg
     4201 ttgttgcctg ccgcatacag cgccccgacg aggctgcccg cgctcgtgcc cacgacgatg
     4261 tcgggaacga taccctgggc ctcgagcgcc ttgatcacgc cgatgtgcgc aaagccgcgc
     4321 gccgcaccgc cgcccagtgc cagcccgatc ttgacggggc ggggagcgac ggcgggctga
     4381 accggcgaca cgacaggagg ggccggcaac tgggttgcgc aactggcgag aaagagtatg
     4441 gcgaggagaa gcagga
//
LOCUS       DATLGF010000251         2079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_26904,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000251 DATLGF010000000
VERSION     DATLGF010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26904"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VM406_07580"
     CDS             <1..313
                     /locus_tag="VM406_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HVL75861.1"
                     /translation="AAEEIERLLPHRIFPGNRPSNMILMERLTPATLGALIALYEHKV
                     FVQGTVWGINSFDQWGVELGKVLAKNIERELEGGAPAFAHDASTTALIARARAVLKAS
                     D"
     gene            331..1794
                     /gene="zwf"
                     /locus_tag="VM406_07585"
     CDS             331..1794
                     /gene="zwf"
                     /locus_tag="VM406_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HVL75862.1"
                     /translation="MAVTDFDMLLFGGGGDLAMRKLLPALYQRDRAGALPATARLLCI
                     GRHDWSREAFLDEIEQNARPHLGEVDPAVWDRFRSRIDYVALDASDPAAYAALHAKVR
                     DDPALTRVFYLATPPGLFAKICHNLAEAGLATPQSRVVLEKPLGRDLESARQINAEVG
                     RVFSESQIFRIDHYLGKETVQNLLALRFGNVFFEPLWRREWISDVQITIAEELGVGGR
                     MDYYETAGALRDMLQNHLLQLLCIVAMEPPISATPDAVRDEKLKVLHSLKRFSASMLA
                     HNVVRGQYRSGHVGGTPVPGYRNEPGANPESRTETFVALKAEIDTWRWAGVPFYLRTG
                     KRMAASLAEIVVRFKNVPHSIFPQPHGSFQPNTLLIRLQPDEGLQLHLMAKTPGDGMR
                     LRPVELELDFRETFRMPRMDAYERLLLDVLRGQLTLFMRSDELEAAWEWVQPILDHWE
                     QEGDNPLPYAAGTWGPAAASALIGRDGFQWREEALPE"
     gene            1804..>2079
                     /locus_tag="VM406_07590"
     CDS             1804..>2079
                     /locus_tag="VM406_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HVL75863.1"
                     /translation="MLLDSIRTQLEALSKSEKKVALAVLKNPDTAVAGNITALAKSAQ
                     VSEPTVVRFCRALGYDGWHEFKLKLAQTLALAVPDTDESPLPDDLAAD"
BASE COUNT          358 a          713 c          671 g          337 t
ORIGIN      
        1 ggcggcggag gagatcgagc gcctgcttcc gcaccgcatc ttccccggca accgcccgag
       61 caacatgatc ctgatggagc ggctgacgcc cgcaacgctg ggcgcgctga tcgcgctcta
      121 cgagcacaag gtgttcgtgc agggaacggt ctggggcatc aacagtttcg accaatgggg
      181 ggtggaactg ggcaaggtgc tcgcaaagaa catcgagcgc gaactcgagg gcggcgcccc
      241 ggcgttcgcg catgacgcct cgaccaccgc cctgatcgcg cgcgcccgcg ccgtgctgaa
      301 ggcttccgac tgaaccaatc cgcgtccacc atggctgtaa ccgatttcga catgctgctg
      361 ttcggcggcg ggggcgatct tgcaatgcgc aagctgctgc cggctctcta ccaacgcgat
      421 cgcgccggcg cgctgcccgc cacggcgcgg ctgctctgca ttggtcgcca cgactggagc
      481 cgggaagcct tcctcgacga gatcgagcag aacgcccggc cgcaccttgg tgaagtggac
      541 ccggcggtct gggatcgctt tcgcagccgc atcgactatg ttgcgctcga cgcaagcgat
      601 cctgccgcct acgccgcgct gcacgccaag gtgcgcgacg atccggcgct cacccgcgtg
      661 ttctacctcg ccaccccgcc cgggctgttc gcgaagatct gccacaacct ggccgaagcc
      721 ggattggcca cgccgcagtc gcgggtcgtg ctcgagaagc cgctgggccg cgacctggaa
      781 agcgcgcgcc agatcaatgc ggaggtcgga cgcgtgttct cggaatcgca gatcttccgt
      841 atcgatcact atctcggcaa ggaaaccgtg cagaacctgc tggccctgcg tttcggcaac
      901 gtgttcttcg aaccgctgtg gcgccgcgag tggatctccg acgtgcagat cacgatcgcc
      961 gaggaactcg gtgtgggcgg gcgcatggat tactacgaga ccgccggcgc gctgcgcgac
     1021 atgctgcaga accacctgct gcagctgctg tgcatcgtcg cgatggagcc gccgatttcg
     1081 gccacgccgg atgcggtgcg cgatgaaaag ctgaaggtgc tgcattccct taaacgtttc
     1141 tctgcgtcga tgctcgcgca caacgtggtg cgcggccagt accgctccgg ccatgtgggc
     1201 ggcacgccgg tgccgggcta tcgcaacgag cccggtgcga atccggagtc gcgcaccgaa
     1261 accttcgtcg cgctgaaggc cgagatcgat acctggcgct gggccggcgt gccgttctac
     1321 ctgcgcaccg gcaagcgcat ggcggcctcg ctggcggaaa tcgtcgtgcg tttcaagaac
     1381 gtgccgcact cgatcttccc gcagccgcac ggcagcttcc agccgaacac gctgctgatc
     1441 cgcctgcaac cggacgaggg cctgcagctg cacctgatgg cgaagacgcc aggcgacggc
     1501 atgcgcctgc gcccggtcga gctcgaactc gatttccgcg aaaccttccg catgccacgc
     1561 atggacgctt acgagcgcct gctgctcgat gtgctgcgcg gccagctcac gctgttcatg
     1621 cgcagcgacg aactcgaagc cgcctgggag tgggtgcagc ccatcctcga ccactgggag
     1681 caggagggcg acaatcccct gccctatgcg gccggcacct ggggcccggc cgcggcgagc
     1741 gcactgatcg gccgcgacgg attccagtgg cgcgaagaag ccttgcccga gtaagctgac
     1801 ggcatgctgc tcgattccat ccgcacccag ctcgaagcgc tttccaaatc ggagaaaaag
     1861 gtcgcgctcg ccgtcctgaa gaatccggat accgccgtag ccggcaacat caccgcgctg
     1921 gccaagagcg cgcaggtgtc cgagccgacc gtcgtgcgtt tctgccgcgc gctcggctat
     1981 gacggctggc atgagttcaa gctcaagctc gcgcagacgc tggcgctggc cgtgcccgac
     2041 accgatgaat caccgctgcc ggacgacctc gccgccgac
//
LOCUS       DATLGF010000252         2521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_89531,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000252 DATLGF010000000
VERSION     DATLGF010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89531"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1751)
                     /locus_tag="VM406_07595"
     CDS             complement(<1..1751)
                     /locus_tag="VM406_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HVL75864.1"
                     /translation="MKRRYLADLSLTLLAAIALAAGGLLYLTGMRELAAWSWMAISGL
                     LFVVTAADIMRTLAQRQIGVDLIALLSIGGALALGEHLTAAVIALMLASGRTLEGYAE
                     RRASRDMAALLEKVPRHANRYEGGAVVQVALDAVRPADRLLVRTGETVPVDGSLLSAR
                     AEIDASALTGESMPVACAVGARVQSGSVNAGAPFDLLATHAAGDSALAAIVRLVRSAQ
                     QSRAPAVRLADRYALLFIPLTLGIAGAAWALSGDPVRALAVVVVATPCPLLLAVPVAM
                     VCAMSRCARHGVLIKHGGALEKLAQAHTLFFDKTGTLTGGQARLVAIETEPDTDPNEL
                     LRLAASLDQMSDHAIARAVVEAARERGLALSLPESVREQPGSGIEGRVDGRRVRLGNS
                     DYAAGTNAPSWLPRFARRIGYEGASAVYVNIDGRLAGALQLADEVRPDTPRALRLLRA
                     AGVARTVMLTGDRTDIAQTIGGALGVDAVMAEQSPGDKLDAIAAARASGVTLMVGDGV
                     NDAPALAAADVGVAMGARGAAAAAEAAQVVLLEDRLDRLAFALRIAQGTRVIALQCVI
                     GGMGLSLLAMVAAALGYL"
     gene            complement(1830..>2521)
                     /gene="glgP"
                     /locus_tag="VM406_07600"
     CDS             complement(1830..>2521)
                     /gene="glgP"
                     /locus_tag="VM406_07600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HVL75865.1"
                     /translation="EAHLAAKRALMEMVRERCGVALAEDAIVLAFARRMTGYKRLDLL
                     FSDIERLRRIARERPLQIVVAGKAHPRDEHGKELISLLHRDLAALAPEVRGAFLPGYS
                     MDVARVMVAGADVWLNTPLPPMEASGTSGMKAAFNGVPSLSVLDGWWMEGCVEGVTGW
                     AIGRPGDDPARHADALYEALEQRVLPLYYGERHAWIQVMKGAIGQTAAFFNSHRMMRR
                     YAAEAYLRLTP"
BASE COUNT          388 a          932 c          873 g          328 t
ORIGIN      
        1 aggtagccga gggccgcagc gaccatcgcc agcagcgaca ggcccatgcc gccgatcacg
       61 cactgcagcg cgatgacgcg cgtgccctgc gcgatgcgca gtgcgaacgc gagccggtcg
      121 agccggtctt ccagcagcac gacctgcgcg gcctcggcag cggcggccgc gccgcgcgcg
      181 cccatcgcca ccccgacatc ggcggccgcg agcgccggcg cgtcgttcac accgtcgccg
      241 accatcagcg tgacgccgga tgcgcgcgcg gcggcgatgg cgtcgagctt gtcgcccggg
      301 ctctgctcgg ccatgaccgc atccacgccg agcgcgccgc ctatcgtctg cgcgatgtcg
      361 gtgcggtcgc cggtcagcat caccgtgcgc gcaacgcccg cggcgcgcaa gagacgcaac
      421 gcacgcggcg tatccgggcg cacttcatcg gccagctgca gcgcgccggc cagccgcccg
      481 tcgatgttca cgtacaccgc cgatgcccct tcgtagccga tgcgccgcgc aaagcgcggc
      541 agccacgacg gcgcgttggt ccccgcggca tagtcgctgt tgccgaggcg cacccggcgg
      601 ccgtcgaccc ggccttcgat gccgctgccg ggctgttccc gcaccgattc aggtagcgac
      661 agcgccagcc cacgctcgcg cgcggcctcg accaccgcgc gcgcgatcgc atggtcggac
      721 atctggtcga gcgaagcggc cagccgcagc aattcgttcg ggtccgtatc gggttcggtt
      781 tcgatcgcaa ccaggcgcgc ctggccgccg gtcagcgtgc cagtcttgtc gaagaacagc
      841 gtgtgcgcct gggccagttt ctccagcgcg ccgccgtgct tgatcagcac gccgtgacgc
      901 gcgcagcgcg acatcgcgca taccatcgcg accggcactg cgagcagaag cgggcagggc
      961 gtggcgacca cgaccaccgc cagcgcccgc accgggtcgc cggacagtgc ccaagcggcg
     1021 ccggcaatgc ccagcgtcag tggaatgaac agcagcgcgt agcggtccgc cagacgcacg
     1081 gccggcgccc tggactgttg cgccgaacgc acgagacgga ctattgccgc cagcgcgctg
     1141 tcgccggccg catgcgtggc cagcaggtcg aacggcgccc cggcgttgac ggagccgctt
     1201 tgcacgcgcg cgcccacggc gcaggcaacc ggcatagact cgccggtcag ggcggaggcg
     1261 tcgatctcgg cgcgggccga cagcaggctg ccgtccaccg gaacggtctc gccggtgcgc
     1321 accagcaggc ggtccgcagg gcgcaccgcg tcgagcgcga cctgcacgac ggcgccgccc
     1381 tcgtaacggt ttgcgtggcg cggcaccttc tcgagcagcg ccgccatgtc gcggcttgcg
     1441 cgccgctcgg catagccctc cagcgtgcgc ccgctggcca gcatcagcgc gatgacggcg
     1501 gcggtcaggt gttcgccgag cgcgagcgca ccgcctatcg acagcagcgc gatcaggtcg
     1561 accccgatct ggcgttgcgc gagcgtccgc atgatgtcgg cggcggttac aacgaacaac
     1621 agcccggaga tcgccatcca tgaccacgcc gcaagttcgc gcatgccggt caggtacaag
     1681 agccctcctg ccgccagcgc gatggcggcg agcagggtca gcgagaggtc ggcgaggtag
     1741 cggcgcttca tgattctgac ccgcgccgcc cggtcaggga gcacacagcg cgctgcgcgg
     1801 agccgcaggc ggccgggccg accaggggat cagggcgtaa gccgcagata cgcctcggcc
     1861 gcgtaacggc gcatcatccg gtgcgagttg aagaatgcgg cggtctggcc gatcgcgccc
     1921 ttcatgacct ggatccaggc gtggcgctcg ccgtagtaca agggcagcac gcgctgttcg
     1981 agcgcctcgt acagggcatc ggcatgccgg gccggatcgt cgccggggcg gccgatcgcc
     2041 caacccgtca cgccctccac gcagccctcc atccaccagc cgtcgagcac agacaggctg
     2101 ggcacgccgt tgaacgctgc cttcatcccg ctggtgccgg aggcttccat cggcggcagc
     2161 ggcgtgttca gccagacatc ggcgccggcg accatcacgc gcgcgacatc catcgaatag
     2221 cccggaagga aagcgccgcg cacttcgggt gcgagtgcgg cgaggtcgcg gtgcagcagc
     2281 gatatcagtt ccttgccgtg ttcgtcacgc gggtgggcct tgccggcaac gacgatctgc
     2341 agcggccgct cgcgtgcgat ccggcgcagc ctctcgatgt ccgagaacag caggtcgagg
     2401 cgcttgtagc cggtcatgcg ccgcgcgaac gcgagcacga tcgcatcctc ggcgagcgcg
     2461 acgccgcagc gctcgcgcac catctccatc agcgcgcgct tggcggccag atgggcctcc
     2521 c
//
LOCUS       DATLGF010000253         4431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_117587, whole genome shotgun sequence.
ACCESSION   DATLGF010000253 DATLGF010000000
VERSION     DATLGF010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117587"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /gene="dnaA"
                     /locus_tag="VM406_07605"
     CDS             <1..1246
                     /gene="dnaA"
                     /locus_tag="VM406_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HVL75866.1"
                     /translation="YWEAPIEVQFVLDPRAGGMRKPGAPAGAAVNGHANGHMNGSAGA
                     QMNGTNGHLTDLLDGPADRVDMMIDVGARREQSKINPVLTFDSFVTGKANQLARAAAI
                     QVANNPGVSYNPLFLYGGVGLGKTHLIHAIGNQVLAENPNAKIRYIHAEQYVRDVVTA
                     YQRKGFDDFKRYYHSLDLLLIDDIQFFAGKSRTQEEFFYAFEALIAAKKQIIITSDTY
                     PKEISGMDDRLISRFDSGLTVAVEPPELEMRVAILLKKAASEGVVFSDDVAFFVAKHL
                     RSNVRELEGALRKILAYSRFHGKDITIDVVKDALKDLLSVQNRQISVENIQKTVADFF
                     NIKVADMYSKKRPANIARPRQIAMYLAKELTQKSLPEIGELFGGRDHTTVLHAVRKIA
                     ADRAKNPELNHELHVLEQTLKG"
     gene            1633..2739
                     /gene="dnaN"
                     /locus_tag="VM406_07610"
     CDS             1633..2739
                     /gene="dnaN"
                     /locus_tag="VM406_07610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HVL75867.1"
                     /translation="MQLVKAPRDTLLRPLQIVSGIVERRHTLPILANILIRKEGEKVS
                     FLSTDIEVQITTHADVGSGGEVAATTVAARKLLDILRALPDSGDVSLSLANKRMTVQA
                     GKSRFALQTLAAEEFPTVQQAEHFNATVKLPQKTLKHLFNMVHFAMAQQDIRYYLNGL
                     LLVVDGRNVIAVATDGHRLAFCQVETEQEFARQEVIIPRKTILELQRLLEDLDDEVQL
                     EIAANQVKLTFADIELISKLVEGKFPDYTRVIPKGYKNDFTIDRQTLQHALQRAAIMT
                     SDKFKGVRCIIAPGSLKISSTNADQEEAVEELEIDYGGDSIDIGFNVTYLLDVLNNLK
                     SDNINVALGDANSSALITVPENADFKYVVMPMRI"
     gene            3036..>4431
                     /locus_tag="VM406_07615"
     CDS             3036..>4431
                     /locus_tag="VM406_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL75868.1"
                     /translation="MSALPQETPREQTQQSQYSASSIQILEGLEAVRKRPGMYIGDTS
                     DGTGLHHLVFEVLDNSIDEALAGHCTEIHVTIHSDNSISITDNGRGIPTGIKWDDKHD
                     PKRSAAEIVMTELHAGGKFDQNSYKVSGGLHGVGVSCVNALSKLLKLTIRRDGKIHQM
                     EFAKGVVQNRELEIQDGVQVSPIKVTGETDKRGTEVHFWADEEIFTHVEFHYDILAKR
                     IRELSFLNNGVHIKLTDQRTGKEEDFAFEGGTRGFVEYINKAKNTLHPTIFQATGEKV
                     SEQGTMIGVDVSMQWNDSFNETVLCFTNNIPQRDGGTHLTGLRAAMTRVINKYIEENE
                     LAKKAKVEISGDDMREGLTCVLSVKVPEPKFSSQTKDKLVSSEVRGPVEEIVARTLTE
                     YLEERPNDAKIIVGKIIEAAKAREAARRAREMTRRKGVLDGLGLPGKLADCQEKDPAL
                     CEIYIVEGDSAGGSA"
BASE COUNT          996 a         1442 c         1297 g          696 t
ORIGIN      
        1 atactgggaa gcgccgatcg aggtccagtt cgtgctcgat ccgcgcgccg gcggcatgcg
       61 caagcctggc gcaccggccg gggcggcggt gaacggccac gccaacggtc atatgaacgg
      121 ctcggccggc gcgcagatga acggcaccaa cggccacctg acggatctgc tcgacggccc
      181 ggcggaccgg gtcgacatga tgatcgacgt cggcgcgcgc cgtgagcaga gcaagatcaa
      241 tccggtgctg accttcgaca gcttcgtgac cggcaaggcc aaccagctgg cgcgcgccgc
      301 cgcgatccag gtcgccaaca accctggcgt gtcctacaac ccgctgttcc tctacggcgg
      361 ggtgggcctg ggcaaaaccc acttgatcca tgcgatcggc aaccaggtcc tggccgagaa
      421 tccaaatgcc aagatccgct acatccacgc ggaacagtat gttcgcgacg tagtgaccgc
      481 ttaccaacgc aagggcttcg acgacttcaa gcgctactat cactcgctcg atctgttgct
      541 gatcgacgac attcagttct ttgccggcaa gagccgcacg caggaggaat tcttctatgc
      601 gttcgaggct ttgattgccg caaagaaaca aatcatcatc accagcgaca cctatccgaa
      661 ggaaatcagc ggcatggacg accgcctgat ttcccgcttc gactccggcc tgacggtcgc
      721 ggtcgagccg ccggagctgg agatgcgggt cgcgatcctg ctgaaaaagg cggcgtccga
      781 gggcgtggtg ttctccgacg acgtcgcatt cttcgtcgcc aagcacctgc gctccaacgt
      841 gcgcgagctc gaaggcgcgc tgcgcaagat cctcgcgtac tcgcgtttcc acgggaagga
      901 catcacgatc gacgtggtca aggatgcact gaaagacctg ctttccgtgc agaaccgcca
      961 gatctcggtc gagaacatcc agaagacggt ggcggacttc ttcaacatca aggtcgccga
     1021 tatgtactcg aagaagcgac cggccaacat cgcccggccg cgccagatcg cgatgtatct
     1081 tgccaaggag ttaacgcaaa agagcctgcc ggagatcggc gagctgttcg gcggacgcga
     1141 ccacaccacc gtcctgcatg cggtgcgcaa gattgcggca gaccgggcga aaaatcccga
     1201 actcaaccat gagctgcacg tactcgagca aacgctgaag ggctgaagag ttgttcatcc
     1261 gttgcccaca ggcttgtgca caacgttatc cacagctgcc taaagggctg tggataagct
     1321 tccggatctg atacaatact gagttcgctc aagcgaggtc cccgggcggc gggaaccgga
     1381 aggagcgggc tggcgccgag tgccggacgg tgttgcggaa gtggcaatat tcggcgccgc
     1441 cgttcatacc gcagaagcgc cagtgaaact tgccaagcgt acaaaaatct ccttgctcaa
     1501 tttctagaag accccccttt tctgtcaaaa tagagcctgt cgcgactatc gtttgacccg
     1561 gccgcgacgc tgctacccaa gacccgggcg gccaccctcc cgggctcgtc tcaaccatca
     1621 caaggacaca gcatgcaatt ggtcaaagcc ccacgcgaca cgcttctgcg tcccttgcag
     1681 atcgtgagcg gtattgtcga gcgtcggcac acgttgccga ttctggccaa tatcctcatc
     1741 cgcaaggaag gcgagaaggt ttcgttcctc tccaccgaca tcgaagttca gatcaccacc
     1801 cacgccgatg tcggttccgg cggtgaagtc gcggccacca cggtcgccgc acgcaagctg
     1861 ctcgacatcc tgcgcgcgct gcccgattcc ggcgacgtct ccctatcgct ggccaacaag
     1921 cgcatgacgg tccaggccgg caagagccgc tttgcgctgc agacgctggc cgccgaggaa
     1981 ttcccgaccg tgcagcaggc cgagcacttc aatgcgacgg tcaagctgcc gcagaagacg
     2041 ctcaagcacc tgttcaacat ggtgcacttc gcgatggccc agcaggacat ccgctactac
     2101 ctgaacggcc tgttgctggt cgtcgacggc cggaacgtga tcgcggtggc caccgacgga
     2161 caccgtctgg ccttctgcca ggtcgagacc gagcaggaat tcgcgcgcca ggaagtgatc
     2221 atcccgagaa agaccatcct cgaactgcag cgtctgctgg aggacctgga cgacgaagtt
     2281 cagctcgaga tcgcggccaa ccaggtcaag ctcacgttcg ccgacatcga actgatttcc
     2341 aagctggtcg agggcaagtt ccccgactac acgcgggtga tcccgaaggg ctacaagaac
     2401 gatttcacga tcgaccgcca gaccctgcag cacgcgctgc agcgcgcggc catcatgacg
     2461 agcgacaagt tcaagggcgt acgctgcatc atcgcgcccg gcagcctgaa gatcagctcg
     2521 accaacgccg accaggaaga ggcggtcgaa gaactggaaa tcgactacgg cggcgacagc
     2581 atcgacatcg gtttcaacgt cacctatctg ctcgacgtgc tgaacaacct caagagcgac
     2641 aacatcaacg tcgcgctcgg ggacgccaat tcgtcggcgc tgattaccgt gccggaaaac
     2701 gcggacttca agtacgtcgt gatgccgatg cggatttgat tggataacga ataaacagca
     2761 agggggcggc gcagatgcgc gccccttgct gcattttagg aagcagaaaa agcaaggccg
     2821 acgaggataa acagggtcgc cggcgcacaa gtcaaccacc ggccggcccc ttcccccttt
     2881 tagggggaag gctgggatgg gggtgcggcg cccgaacagg caaggttgcc ggattcgagc
     2941 gcacgacccc caccccgacc ctccccctgc aacggggagg gaacccgtca gcagcaagca
     3001 acaaacagca ccacccgttc tttcagaagg tacccatgtc cgcactccct caagaaaccc
     3061 cgcgcgagca gacgcagcag agccagtaca gcgcctcctc gatccagatc ctcgaaggcc
     3121 tggaggccgt gcgcaaacgt cccggcatgt acatcggcga cacttctgac ggcacgggcc
     3181 tgcaccacct cgtcttcgaa gtgctcgaca actcgatcga cgaagcgctg gccggtcact
     3241 gcaccgagat ccacgtcacg atccactccg acaactcgat ctcgatcacc gacaacggcc
     3301 gcggcatccc caccggcatc aagtgggacg acaagcacga cccgaagcgc agcgccgccg
     3361 aaatcgtgat gaccgagctg cacgccggcg gcaagttcga ccagaactcg tacaaggtct
     3421 ccggcggcct gcacggcgtc ggcgtgtcct gcgtcaacgc actgtccaag cttctcaagc
     3481 tcaccatccg ccgcgacggc aagatccacc agatggaatt cgccaagggc gtggtgcaga
     3541 accgcgaact cgaaatccag gacggcgtgc aagtctcgcc gatcaaggtc accggtgaaa
     3601 ccgacaagcg cggcaccgaa gtgcacttct gggccgacga ggaaatcttc acccacgtcg
     3661 aattccacta cgacatcctg gccaagcgca tccgcgaact ctcgttcctg aacaacggcg
     3721 tgcacatcaa gctgaccgac cagcgcaccg gcaaggaaga agacttcgcg ttcgaaggtg
     3781 gcacgcgcgg cttcgtcgaa tacatcaaca aggccaagaa cacgctgcac ccgaccatct
     3841 tccaggccac cggcgagaag gtgtccgagc agggcacgat gatcggcgtg gatgtctcga
     3901 tgcagtggaa cgactcgttc aacgagaccg tgctctgctt caccaacaac atcccgcagc
     3961 gcgacggcgg cacccatctc acgggtctcc gtgccgcgat gacgcgcgtg atcaacaagt
     4021 acatcgaaga aaacgagctg gccaagaagg ccaaggtcga aatctccggt gacgacatgc
     4081 gcgaaggcct gacctgcgtg ctctcggtga aggtgcccga accgaagttc agctcgcaga
     4141 ccaaggacaa actcgtgtcc tccgaagtgc gcggcccggt cgaggagatc gtggcgcgca
     4201 cgctcaccga gtacctcgag gaacgcccga acgacgccaa gatcatcgtg ggcaagatca
     4261 tcgaagccgc caaggcgcgg gaagccgcgc gccgcgcgcg cgagatgacg cgccgcaagg
     4321 gcgtgctcga cggcctgggc ctgcccggca agctcgccga ctgccaggag aaggacccgg
     4381 cgctgtgcga gatctacatc gtcgagggcg actccgccgg cggttccgcc a
//
LOCUS       DATLGF010000254         2775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_57800,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000254 DATLGF010000000
VERSION     DATLGF010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57800"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..2630)
                     /gene="mutS"
                     /locus_tag="VM406_07620"
     CDS             complement(6..2630)
                     /gene="mutS"
                     /locus_tag="VM406_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HVL75869.1"
                     /translation="MAKHTPMMQQYLRIKAEHPAMLVFYRMGDFYELFFDDAEKAARL
                     LGITLTQRGNSNGAPIKMAGVPFHSVEQYLAKLIKLGESVAICEQIGDPATSKGPVER
                     KVVRVVTPGTLTDSDLLPEKSERPLLALCTQTQRKTITAGLAWLSLASGALKLMEFSC
                     EASHFDARLKQELERIAAAEILVGEDIPLLHMQASGKFTAVPPWHFDAASGRTALLEQ
                     LGVATLSGFGAEQLGPAIGAAGALLRYAQSTQGRGLQHVRTLTVESENEFIGLDAATR
                     RNLELTETLRASEGSNSPTLFSLLDHCRTPMGSRLLRYWLHHARRDQEVAKARHAALN
                     ALMRDDAESGLAAALSAVPDIERITTRVALQSARPRDLAGLRAGLQQLPALRACVAIC
                     NQDAEAPLLLQIHDALATPAECLDLLERSIATEPAAQVRDGGVIARGFDAELDELRAL
                     SENAGQFLVDLETRERARTGIANLRVEYNKVHGFYIEVTNGQTDKVPDDYRRRQTLKN
                     AERYITPELKAFEDKALSAQERALAREKHLYERVLADLGVHIGTLQAIAHALAQLDAL
                     VALAGHAMRNNWCAPQLVAEPVLKIERGRHPVVENQIERFIANDCMLDEERKLLLITG
                     PNMGGKSTYMRQVALITLLAYVGSFVPADSATIGPIDRIFTRIGAADDLAGGRSTFMV
                     EMTESAAILNGATERSLVLMDEVGRGTSTFDGLALAWAIARHLIETTKSFTLFATHYF
                     ELTQLPETHPGAANVHLSAVEHKDSIVFLHAVEPGPASQSYGLQVAQLAGVPQSVIRA
                     ARRHLAALEAQSLQPTPQFDLFAAAPPADECEAEKAVPPQAAASVLAALEETDPDSLT
                     PREALALVYRLRDLATTL"
BASE COUNT          439 a          891 c          930 g          515 t
ORIGIN      
        1 gcgcttcata gggtcgtagc aagatcgcgc aagcggtaga cgagtgcaag cgcttcgcgc
       61 ggtgtcaggg aatccggatc ggtctcttcc agcgcagcca gcaccgaggc cgccgcctgc
      121 ggtggcaccg ctttttccgc ctcgcactcg tcggcaggcg gcgcggcggc aaacaggtcg
      181 aactgcggcg tgggttgcag cgattgcgcc tccagcgctg cgaggtggcg tcgggccgcg
      241 cggatcaccg actgcggcac gccggccagc tgcgcgacct gcaggccata gctctgcgag
      301 gccgggccag gctcaaccgc atgcaggaag acgatgctgt ccttgtgctc gaccgcggac
      361 aggtgcacat tggccgcgcc cggatgcgtc tcgggcagct gcgtgagttc gaaataatgc
      421 gtggcgaaca gcgtgaagct cttcgtcgtc tcgatcaggt ggcgcgcgat ggcccaggcc
      481 agcgccagcc cgtcgaaggt cgaggtgccg cgcccgactt cgtccatcag gaccagcgag
      541 cgttcggtcg cgccgttcag gatcgccgcg gattccgtca tctcgaccat gaaggtcgag
      601 cgaccgccgg ccaggtcgtc agctgcgccg atgcgggtga agatgcggtc tatcggcccg
      661 atggtcgcgc tgtcggccgg tacgaagctg cccacatacg cgagcagcgt gatcaatgcc
      721 acctgccgca tgtaagtcga tttgccgccc atgttcgggc cggtgatcag cagcagcttg
      781 cgctcttcgt cgagcatgca gtcattggcg atgaagcgct cgatctggtt ttcgacgacc
      841 gggtggcgtc cgcgctcgat cttgagcaca ggctcggcga ccagttgcgg cgcgcaccag
      901 ttgttgcgca tcgcatgacc ggccagcgcg accagcgcat caagttgcgc cagcgcatgg
      961 gcgattgcct gcagcgtgcc gatatgcact ccgagatccg ccagcacgcg ttcatacagg
     1021 tgcttctcgc gcgccagcgc gcgctcctgc gctgacaagg ccttgtcctc gaaggctttc
     1081 agctccggcg tgatatagcg ttcggcattc ttcaacgttt ggcgacggcg gtaatcgtcc
     1141 ggcaccttgt cggtctggcc gttggtcacc tcgatataga agccgtgcac cttgttgtat
     1201 tcgacgcgca gattggcgat gccggtgcgc gcgcgttcgc gtgtttccag gtcgaccagg
     1261 aactgtccgg cgttttccga cagcgcgcgc agctcgtcga gctcggcatc gaagccgcgc
     1321 gcgatcaccc cgccgtcacg aacctgcgcg gccggctcgg ttgcaatcga ccgttccagc
     1381 aggtcgaggc attcggccgg agtcgcgagc gcgtcatgga tctgcagcag caggggcgct
     1441 tccgcatcct ggttgcagat cgccacgcag gcgcgcagcg ccggcagctg ctgcaggccg
     1501 gcgcgcaggc ctgccaggtc gcgcgggcgc gcgctttgca gggccacgcg cgtggtgatg
     1561 cgttcgatgt cgggaacggc cgacagtgcc gcggcgagcc ccgattcggc atcgtcgcgc
     1621 atcaaggcat tgagcgcggc atggcgcgcc ttcgccacct cctgatcgcg ccgcgcatgg
     1681 tgcagccagt agcgcagcag gcgcgacccc atcggggtgc ggcagtggtc gagcagcgag
     1741 aacagcgtgg gagaattcga gccttccgat gcccgcaggg tttcggtcag ttccaggttg
     1801 cgccgcgtgg ccgcgtccaa tccgatgaat tcgttctcgc tctcgacagt gagcgtgcgc
     1861 acatgctgca ggccgcgacc ctgtgtcgac tgcgcgtagc gcagcagcgc gcccgcggcg
     1921 ccgatggccg gaccgagttg ttcggcccca aagccgctca gcgtggccac acccagctgc
     1981 tcgagcaggg cggtgcgacc ggatgcggca tcgaaatgcc atgggggaac ggcggtgaac
     2041 ttgccgctcg cctgcatgtg caacagcggg atgtcttcac cgaccagaat ttctgccgcg
     2101 gcgatgcgct caagctcctg tttgagtcgc gcgtcgaaat ggctggcttc acagctgaat
     2161 tccatcagct tcaacgcacc gctggccagc gacagccacg ccaggccggc cgtgatggtc
     2221 ttgcgctggg tttgcgtgca cagcgcgagc agcgggcgct ccgatttctc tggaagcagg
     2281 tccgaatcgg tcagtgtgcc gggcgtgacg acgcgtacga ccttgcgttc gaccggcccc
     2341 ttgctggtgg ccggatcgcc gatctgttcg cagatcgcga ccgactcgcc gagcttgatc
     2401 agcttggcca ggtattgctc gacggagtga aacggcacgc ccgccatctt gatcggcgcg
     2461 ccgttcgaat tgccgcgctg ggtcagcgtg atgccgagca ggcgcgcagc cttctcggca
     2521 tcgtcgaaga acagttcgta gaagtccccc atccggtaga acaccagcat cgccgggtgt
     2581 tcggccttga tgcgcaggta ctgctgcatc attggcgtgt gtttcgccat tgcggaatcc
     2641 gcaacgacgg attcgggaac tgttttcagg gcagatgctt gtgccatgcg ggatctttgc
     2701 ggcgtcattg cgcgggaata cgacgcgtca ttttaatggc gaacgtccgc gtcctctacc
     2761 gtccattgcc ctgcg
//
LOCUS       DATLGF010000255         8116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_167921, whole genome shotgun sequence.
ACCESSION   DATLGF010000255 DATLGF010000000
VERSION     DATLGF010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 8116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8116
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167921"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /locus_tag="VM406_07625"
     CDS             complement(<1..174)
                     /locus_tag="VM406_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HVL75870.1"
                     /translation="MSWLILFVAGLMEIVWAVGLKYTQGFTRLGPSVLTLSAMVASVV
                     LLGMAMKTLPIGTA"
     gene            486..695
                     /locus_tag="VM406_07630"
     CDS             486..695
                     /locus_tag="VM406_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75871.1"
                     /translation="MLKFEFAIATRSGQKIDSIVIAGRDQADAQRKLVQMYRHCTVLH
                     CTARHPDDRLRQSASMEDILTLISK"
     gene            complement(697..1461)
                     /locus_tag="VM406_07635"
     CDS             complement(697..1461)
                     /locus_tag="VM406_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HVL75872.1"
                     /translation="MSIVAVFNQKGGVGKTTTALNLAAALARRGRAPCGFDLDPQAHF
                     SAIAGITAASGEETLLTLFQRNRPLGELMRAAENGIQVLPAHMELSKVDTLFGKGYNV
                     VNRLNTALRSEGLCGSPVPVLIDCSPLIGVLSLNAIFASDCLIVPVSADHLSYKSALQ
                     VDRALTALEPVLKRRVRRRYLLTRFDARRRMAREIQQMMEERFGDDVCKTRIGENVSL
                     AESPAWNRTVFEHAPASRGAQDYEALLDELLRDGVF"
     gene            complement(1703..2275)
                     /locus_tag="VM406_07640"
     CDS             complement(1703..2275)
                     /locus_tag="VM406_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75873.1"
                     /translation="MPRHLFLAALLIPSLAHADFNGAWVGWICPSGVERSSGQCANFV
                     LELMEKDGTLCGTHMFATAGAERIDEGGAPSLTGEVTEAAASGVAVSTLGPQPVRVRV
                     ALRVDGNTLRWERLDNPSGETLLPKQARLTRSRSRTLFAPLFEQELRAACTYVFNLAA
                     AAREPAIPGSAGEGAPAAPEAAPAAPPSAR"
     gene            complement(2343..2948)
                     /locus_tag="VM406_07645"
     CDS             complement(2343..2948)
                     /locus_tag="VM406_07645"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HVL75874.1"
                     /translation="MPDSPLHPSGPHAGLIRCGWANPANPRYLAYHDEEWGVPCHDEA
                     RLFEMLNLEGAQAGLSWETILNKRAGYRAAFDDWDPEKIARYDATRVAQLLANPSIVR
                     NRLKVAAAITNAQAYLRLRDERGGLAPLLWDYVDGKPLQNAPRSLAEVPARTALSDRI
                     AKDLSRRGFKFVGSTIVYAYMQAVGLVNDHCVDCFRHAQLR"
     gene            complement(2991..4265)
                     /locus_tag="VM406_07650"
     CDS             complement(2991..4265)
                     /locus_tag="VM406_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HVL75875.1"
                     /translation="MTDLHAFKAGAKNWIRSASLAAVVAMAAAFATPAQASSHVVLDA
                     TLNERVVMVPAISGGQRIQLETTIFTPPGNGPFPVLIMNHGKAMGSPRAQNRDRFLVV
                     SREFVRRGYAVVVPMRKGFSKSTGDYADRGCNMTANGHIQAEDLFGTLNYLRGQSWAD
                     ANRVVVAGQSYGGLTTMAFGTRNYPGVRGLINFAGGLKVHGGTCRWQDSLVQAFHSYG
                     ARSSVPSLWFYGENDGHFDHELAGRMHTAYTRAGGNARLVAFGPFKSDAHSMIGSRDG
                     VRIWLPETERFLASIGMPSAPVIALADDNRFPATGYAEVDNLEAVPFLRDHGREQYRA
                     FLSKTFPRAFAVSPTGAWSWAEEGDDPAAQALANCEKNSSQACHLYAVDNRVVWAPEP
                     AAPVLADTAPATPGQSATQTGSGQQADGVTGR"
     gene            4661..4912
                     /locus_tag="VM406_07655"
     CDS             4661..4912
                     /locus_tag="VM406_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6139 family protein"
                     /protein_id="HVL75876.1"
                     /translation="MKVDVYRRPQAEGQYSYLAVPEGRPIPNEATAIDWETALNNVDL
                     DDGNEPVDGLPASDALQQINAKGYAISHAPNNGMQRGSR"
     gene            5051..6466
                     /gene="cls"
                     /locus_tag="VM406_07660"
     CDS             5051..6466
                     /gene="cls"
                     /locus_tag="VM406_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010396359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HVL75877.1"
                     /translation="MDRFPFLASLARLRIAQAALLCALAGCATTLPDVTHLRERLSGT
                     PAPTIVTSGGEVPPKRREAMLDALAERVGPTDILAHHVAAEERIAGRPLVAGNKVALL
                     DDGPATMRAMREAIAAASDHVNLETYIFDADEVGHELADLLIEKRRAGVVVNVIYDSV
                     GALDTPREFFERMRAAGIAVLEYNPVNPLYARGRWEVNQRNHRKILVVDGQVAFTGGV
                     NISKVYGKSSLSAGSGEPPPDARDAAWRDTHMRIEGPVVADFQEKFLDAWLRQTGHLP
                     PPANYFPPPRQAGNALVRAIGTTPDRGHHAVYKTYISAFVHADRYIHITTAYFVPDRE
                     VLQAITDAARRGVDVKIIFPSFSDVSLVLHAGRSFYDELLAAGVKIYERKGAMLHAKT
                     AVIDGVWSTIGSTNLDMRSFLHNDEINAVILDADFARQMETLFQRDLRDSAEIREEEW
                     KQRGLRVRMREWAVRLFSYWL"
     gene            complement(6527..6931)
                     /gene="pcaC"
                     /locus_tag="VM406_07665"
     CDS             complement(6527..6931)
                     /gene="pcaC"
                     /locus_tag="VM406_07665"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HVL75878.1"
                     /translation="MDERNKDEQYKDEQYERGLRMRRQVMGDAFVDRAFAGADEFTMP
                     LQEFITRNAWGTVWCRDGLDLKTRSLITVAMLTALGRTHELKGHLRGALNNGATREEL
                     RELLLHAAVYCGAPAAVEGFRAAQEVLGPSPE"
     gene            complement(6909..>8116)
                     /locus_tag="VM406_07670"
     CDS             complement(6909..>8116)
                     /locus_tag="VM406_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL75879.1"
                     /translation="EPWRIVHLLAVTQIISYGSLFYAFALLAPAIRDEFGWRNELVFG
                     AFSLSLLVAGLGASPAGALIDRHGGRSVMAAGSVLAALGLLGAAMARSLPGYYLAWML
                     IGAAMATVLYEAAFATINAAFETGARRAVSVLTLYGGFASTVFWPLTLQLNGTLGWRG
                     TFAAYAALQLCVCLPAHLAMRGRAGRAATGAGTPAHGHTLAEALRDPVFWKLACAFAT
                     NMFIFSAMSVHVIPLLQRFGHGVGTAVFLAALIGPMQVAGRLGEMTLASRFAPAAVGR
                     VTFALLPAGLLALLMLGERQFAAALFCILYGMSNGIMTIVRGTVPIELFGRQNYGAIA
                     GALAGPTLIAKAAGPLVLAALVEFFPGDRWLVAFLLAVALLSMAAFLAALSGRRPTTE
                     REHGRTEQR"
BASE COUNT         1459 a         2662 c         2671 g         1324 t
ORIGIN      
        1 ggcggtgccg atcggcagcg tcttcatcgc catgccgagc aggaccacgc tcgcaaccat
       61 tgccgacagg gtcagcaccg aagggccgag ccgggtgaaa ccctgggtgt acttgagccc
      121 gacggcccag acgatttcca tcaaacccgc gacaaacagg atcagccagg acatgaatgc
      181 ctcttggaat tttcgtggcc gtccacgatc aggggaattc aggccagggt cgtccccggc
      241 ccggccgcag cgccgccgga accggcgggc atgctggcgt tgaccattcc gcctcatgct
      301 tcgcggaccg tcatgaacgg gtgcgggcgc ggcggattgc agaagtggga agtctaaccg
      361 atgcaggaaa agctttcagg ccgcgccccg gcgaccagtc cccggcgccg cggcgcgtcg
      421 taaccttgtt gccaacaggc atgcatgccg ttataaaacc ggttacacga caccggggag
      481 aagcgatgct caaattcgaa ttcgcaatcg cgacgcgcag cggccagaaa atcgacagca
      541 tcgtcatcgc gggacgcgac caggccgatg cgcagcgcaa gctggtgcag atgtacaggc
      601 actgcacggt gctccactgc acggcacgcc accctgacga caggctccgc cagtccgcct
      661 cgatggagga cattctgacg ctgatctcga agtgattcag aacacgccgt cgcgcagcag
      721 ctcgtcgagc agcgcctcat aatcctgcgc gccgcggctg gccggcgcgt gctcgaacac
      781 ggtcctgttc caggccggac tctctgccag gctcacgttc tccccgattc gcgtcttgca
      841 gacgtcatcg ccgaagcgtt cctccatcat ctgctggatc tcgcgcgcca tgcgccgccg
      901 ggcatcgaac cgggtcagca ggtagcggcg ccggacgcgg cgcttgagca ccggctccag
      961 cgcagtcagc gcgcgatcga cctgcagcgc gcttttatag gacaggtgat cggccgagac
     1021 cggcacgatc aggcagtcgc tggcgaagat cgcgttgagc gacagcacgc cgatcagcgg
     1081 tgagcagtcg atcagcaccg gcaccgggct gccgcacagg ccctcgctgc gcagcgcagt
     1141 gttgagccgg ttgaccacgt tgtagccctt gccgaacagc gtatcgacct tggagagttc
     1201 catgtgggcc ggcagtacct ggatgccgtt ttcggcggcc cgcatcagtt cgcccagcgg
     1261 ccggttgcgc tggaacaggg tcaacagcgt ctcctcgccg gatgccgcgg tgatgccggc
     1321 gatcgccgag aaatgggcct gcggatcgag atcgaaaccg cagggcgcgc gcccacggcg
     1381 cgccagcgcg gcggcgaggt tgagggctgt ggtggtcttg cccacgcccc ccttctggtt
     1441 gaagacggcg acgatgctca tggcgtgcgg ccatcttact gcagaaccac ggacttgtaa
     1501 ctttcaggca acttcccggc gggcaaaaaa gggagtggag gcggcgcgcc gcctccactc
     1561 caaacctcag gagacactga ggagagagtc gggcattgcc gccgcaaagc gctgcggcgc
     1621 tgcccgaccg gagcaatgta gcagcgcttt tgccgcagaa aatccggaaa gcagcccgct
     1681 gcggccaaga agattctgcg gttcagcgcg cagagggtgg cgcggccggc gccgcttccg
     1741 gtgcagccgg agcgccctcg cctgcgctgc cgggaatcgc cggctcgcgc gcggccgccg
     1801 ccagattgaa cacataggta caggccgcgc gcaattcctg ctcgaacagc ggcgcaaaca
     1861 gcgtgcgact tcttgaacgg gtcaggcgcg cctgtttggg cagcagcgtc tcgccggacg
     1921 gattgtccag ccgctcccag cgcagtgtgt tgccatcgac cctgagtgcc acgcgtacgc
     1981 gcaccggctg cggccctagc gtgctgaccg ccacgccgct ggccgccgcc tccgtaacct
     2041 cgccggtcag cgacggggcg ccgccctcgt cgatgcgctc ggcgccggcg gtggcgaaca
     2101 tgtgcgtgcc gcacagcgtg ccgtcctttt ccatcagttc gagcacgaag ttcgcgcatt
     2161 gccccgaact gcgctcgacg ccggacggac agatccagcc aacccaggcg ccattgaagt
     2221 cggcgtgggc cagcgacgga atcagcaagg cggcgagaaa caggtgtctt ggcatggctg
     2281 ggacgtggat cggaaccact atcccgcgtt gcgcctgggc gtgccttgag tcctcgcaac
     2341 gctcagcgca gctgggcgtg acggaaacaa tcgacgcagt gatcgttcac caggcccacc
     2401 gcctgcatgt atgcatagac gatggtcgag ccgacgaact tgaagccgcg ccgcgacaag
     2461 tccttcgcaa tgcggtcgga caacgcggtg cgtgccggca cctcggccag gctgcgcggc
     2521 gcattctgca agggcttgcc gtccacataa tcccacagca gcggcgccag gccgccgcgc
     2581 tcgtcgcgca ggcgcaggta ggcctgggca ttggtgatcg cggcggccac cttcagccgg
     2641 ttgcgcacga tggatgggtt cgcgagcagc tgcgcgacgc gcgtggcgtc gtagcgggca
     2701 atcttctccg ggtcccagtc gtcgaatgcg gcgcgatagc cggcgcgctt gttgaggatg
     2761 gtctcccagc tcaggcccgc ctgcgcgcct tccaggttga gcatctcgaa caggcgggcc
     2821 tcgtcatggc agggcacgcc ccactcttca tcatggtagg cgaggtagcg cggattggcg
     2881 ggattggccc agccgcaacg aatcaggccc gcatgcgggc ctgatggatg gaggggggaa
     2941 tcgggcatgt tgtccttccg gtcgtgtcgg atggacaagc atagcaggcg ctatcgcccg
     3001 gtcacgccgt cggcctgctg gccggaaccg gtctgggtgg cagactggcc gggcgtggcc
     3061 ggcgcggtgt cggccagtac cggcgcagcc ggctcgggag cccataccac gcggttgtcg
     3121 accgcataca gatggcaggc ctggctgctg ttcttctcgc aattcgccag cgcttgcgcg
     3181 gccggatcgt caccctcttc cgcccagctc caggcaccgg tcggggacac ggcgaacgcg
     3241 cgcgggaagg tcttggacag gaacgcgcgg tattgctcgc ggccatggtc gcgcaggaag
     3301 ggcactgcct cgaggttgtc cacttccgca tagccggtgg ccgggaagcg gttgtcgtcg
     3361 gccagcgcga tcaccggcgc gctcggcatg ccgatcgagg cgaggaaacg ctcggtttcg
     3421 ggcagccaga tgcgcacgcc gtcgcggctg ccgatcatgc tgtgcgcatc gctcttgaac
     3481 ggtccgaacg cgaccaggcg cgcgttgccg ccggcgcggg tgtaggcagt gtgcatgcgc
     3541 ccggcgagtt catggtcgaa gtgaccatcg ttctcgccgt agaaccacag gctcggcacg
     3601 ctgctgcgcg cgccgtagct gtggaaggcc tgcaccagcg aatcctgcca gcggcaggtg
     3661 ccgccgtgta ccttcagccc gccggcgaaa ttgatcaggc cgcgcacgcc gggatagttg
     3721 cgggtgccga aggccatcgt ggtcaggccg ccataggact ggcccgcgac caccacgcga
     3781 ttggcgtcgg cccagctctg gccgcgcaga tagttcagcg tgccgaacag atcttcagcc
     3841 tgaatgtggc cgttggccgt catgttgcaa ccacggtcgg catagtcgcc ggtagacttg
     3901 gaaaagccct tgcgcatcgg cacgacgact gcatagccgc ggcgcacgaa ttcacggctg
     3961 acgaccagga agcggtcgcg gttttgcgca cgcgggctgc ccatggcctt gccgtggttc
     4021 atgatcagca ccgggaacgg gccgttgccc ggcggggtga agatggtggt ttcgagctgg
     4081 atgcgctggc cgccggaaat cgccggcacc atcactacgc gttcgttgag cgtggcatcc
     4141 aggacaacat gggagcttgc ttgggcgggt gtagcgaagg ccgctgccat ggcaacgacg
     4201 gcagcgaggg aagcggagcg gatccagttc ttcgcgccag ccttgaatgc gtgtagatcg
     4261 gtcatgcgtg ctctccgtca aatctgcaaa cacggctcgc gcacgagccg ccggatgggc
     4321 ttcgccggac ttgctgactt gaaacggcgc accttcaggt gcgctcacgc gagtgggggc
     4381 gaagcgcgcg ctacgggtgt tgtcctgcgt aatcgcttct tcttgacgta gcgcaacgtt
     4441 gcccattcta aacacgggtt ttctgtggtc aatacgcgtt tatgcaactc ggaaaggttt
     4501 ttttgacgaa agttttccct atgaaaacga atgttttcga gagaagaaga cggaggggaa
     4561 agagaggaag gattgccggc gaaaaaggcg ttaccgcgca acctttgccg atggcagagt
     4621 gtccattagg gacacctttt ttgaaggagg aaaggaagtc atgaaagtgg atgtctatcg
     4681 ccgcccccag gcagaaggcc agtactccta ccttgccgtc ccggaaggcc gaccgatccc
     4741 gaacgaggcc acggcaatcg actgggagac tgccttgaac aacgtcgatc tggatgacgg
     4801 gaatgagccc gtcgacgggt tgcccgcctc ggacgccctg cagcagatca atgccaaggg
     4861 gtatgcgatc tcgcatgcac ccaacaacgg catgcaaaga ggttcacgct gacttgcgag
     4921 cgggcattgc ccgctcttcc tttctcgatg tccgcgttgc gaatccacaa tggcgcgcgg
     4981 gaattatcgt tcttgaaacc ggctccaatc cgcatgggcg cacacctcgc ttgccgctgc
     5041 ggatgtggga atggaccggt tcccgttcct tgctagcctt gcgcgtcttc ggatcgcgca
     5101 ggccgccctg ctatgcgcgc tcgccggatg cgcgaccacg ctgcccgacg tcacccatct
     5161 gcgagagcgc ttgtccggca cgcctgctcc cacgatcgtc acttccggcg gagaggttcc
     5221 gccgaagcgg cgtgaagcga tgctcgatgc gctggccgaa cgtgtgggtc cgaccgacat
     5281 ccttgcgcac catgtcgccg ccgaggagcg catcgcgggc cgccctctcg tcgccggcaa
     5341 caaggtcgcc ctgctcgacg acggcccggc aaccatgcgc gcgatgcgcg aggcgattgc
     5401 cgccgcgagc gatcatgtca acctcgaaac ctatatcttc gatgccgatg aggtcggcca
     5461 tgagctggcc gacctgctga tcgagaagcg ccgcgcgggt gtggtggtca acgtgattta
     5521 cgacagcgtc ggcgccctcg acacgccgcg cgagttcttc gagcggatgc gcgcggccgg
     5581 catcgcagtg ctcgagtaca acccggtcaa tccgctttac gcccgcggcc gctgggaagt
     5641 gaaccagcgc aatcaccgca agatcctggt cgtcgacgga caggtcgcgt tcaccggcgg
     5701 cgtcaacatc agcaaggtgt acggcaagag ctcgctgtcg gcgggttcgg gcgagcctcc
     5761 gcccgatgcg cgcgacgccg cctggcgcga tacccacatg cgcatcgagg gaccggtcgt
     5821 cgccgatttc caggaaaaat tccttgatgc ctggctgcgc cagaccggac acctgccgcc
     5881 gccggccaac tactttccgc cgccgcggca ggccggcaac gcgctggtgc gcgcgatcgg
     5941 caccacgccg gaccgcggcc atcatgcggt gtacaagacc tacatctcgg ccttcgtgca
     6001 tgcggatcgc tacatccaca tcaccaccgc ctatttcgtg cccgatcgcg aagtgcttca
     6061 agcgatcacc gatgcggcgc gccggggcgt ggacgtgaag atcatctttc cgagtttttc
     6121 cgacgtcagc ctggtgctgc atgcgggccg ctcgttctac gacgaactgc ttgcagccgg
     6181 cgtgaagatc tacgaacgca agggtgcgat gctgcatgcg aagaccgcag tcatcgacgg
     6241 cgtctggtcg accataggct cgaccaatct cgacatgcgc agcttcctgc acaacgacga
     6301 gatcaacgcc gtcatcctcg atgccgactt cgccaggcag atggagacgc tgttccagcg
     6361 cgacctgcgc gattcggccg agatccggga ggaagagtgg aagcagcgcg gactgcgcgt
     6421 acgcatgcgc gaatgggcgg tgcggctgtt ttcatattgg ctttagcagc cagacaggtc
     6481 caggcaccgc aaggaaattg ccacatcgct cactggaacc atccgcctat tccggcgacg
     6541 gacccaggac ttcctgcgcg gcacggaagc cttcaaccgc tgccggcgcg ccgcaataga
     6601 ccgccgcgtg caacagcaac tcgcgcaact cctcgcgcgt cgcgccattg ttgagcgcgc
     6661 cgcgcagatg ccccttcagc tcatgcgtgc gcccgagcgc ggtcagcatc gcaaccgtga
     6721 tcaggctgcg cgtcttgaga tcgagaccgt cgcgacacca gaccgtgccc caggcgttgc
     6781 gcgtgatgaa ttcctgcagc ggcatcgtaa attcgtccgc gccggcgaac gcgcggtcga
     6841 cgaacgcgtc acccatcacc tgcctgcgca tgcgcaggcc cctttcgtac tgctcatcct
     6901 tgtattgttc atctttgttc cgttcgtcca tgttctcgct cggtagtggg gcggcgcccg
     6961 gacagcgccg ccaggaaggc ggccatcgac aacagcgcga ccgccagcag gaaggcgacc
     7021 agccagcggt cgcccgggaa aaattcgacc agcgcggcga gcaccagcgg ccccgccgcc
     7081 ttggcgatca gcgtgggccc ggccagcgcg ccggcgatgg cgccgtagtt ctgccggccg
     7141 aacagttcga tcggcacggt gccgcgcacg atggtcatga tgccgttgct catgccgtag
     7201 aggatgcaga agagcgcagc ggcgaactgg cgttcgccga gcatcagcaa ggccagcagg
     7261 cccgcgggca gcagcgcgaa cgtgactcgg ccgaccgcgg ccggcgcgaa tcggctggcc
     7321 agcgtcattt cccccagccg gccggccacc tgcatcgggc cgatcagcgc ggcgagaaag
     7381 accgcggtgc cgacgccgtg gccgaagcgc tgcagcagcg gaatcacgtg caccgacatc
     7441 gccgagaaaa tgaacatgtt ggtggcgaac gcgcaggcga gcttccagaa caccggatcg
     7501 cgcagggctt cggcgagcgt gtgcccgtgg gccggcgtcc ctgcacctgt cgccgcgcgg
     7561 ccggccctgc cgcgcatcgc cagatgggcc ggcaggcata cgcatagctg cagcgccgca
     7621 taggcggcga aggtgccgcg ccatccgagc gtgccgttca gctgcaaagt caggggccag
     7681 aacacggtgc tggcaaagcc gccgtacagc gtgagcacgc tgacggctct gcgggcgccg
     7741 gtctcgaatg ccgcgttgat ggtggcaaag gctgcctcgt acagcaccgt cgccatcgcc
     7801 gcgccgatca gcatccatgc gaggtagtag ccgggcaggc tgcgcgccat cgcggctcca
     7861 agcaggccga gcgcggcaag aaccgagccg gcggccatca cgcttcgccc gccatgcctg
     7921 tcgatcagcg ccccggccgg cgaagcaccc agacccgcca ccagcaggct cagcgagaag
     7981 gcgccgaaca ccagttcgtt gcgccagccg aattcatccc gtatggcggg cgccagcagc
     8041 gcgaacgcat agaacagcga accgtaggaa atgatctgcg tgacggccag caggtggacg
     8101 atgcgccaag gttcgc
//
LOCUS       DATLGF010000256         3313 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_89752,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000256 DATLGF010000000
VERSION     DATLGF010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89752"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1868
                     /gene="glnE"
                     /locus_tag="VM406_07675"
     CDS             <1..1868
                     /gene="glnE"
                     /locus_tag="VM406_07675"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HVL75880.1"
                     /translation="QEARHPERSHNVKLGRGGIREIEFLAQVFQLIRGGRDTELRDRS
                     TRRTLDMLAHKQLLSADDVAQLQAAYAFLRNLEHRLQYREDAQTHTLPPGDEERLVVA
                     QMMGCATVDELLAELGRERAIVAAQFDAMFSEKGSDDGDSDEAAHAPLSETEGVDSLE
                     ARLQDCGFPAPREAAQRLMATIQSAKLHSLPAASRTKLLALIHTALPLIGEEPATATA
                     TLGRLLDFLEAIARRAAYLALLTEYPHTLERVIRMMCASRWAAQYLTRHPILLDELLE
                     DRTLHAAPDWPAFAQEVQRRLEAVEGDTEMQMNVLRELHHAQLFRLLAQDLEGMLTVE
                     KLADHLSALADILVHATIGAAWKSIPGRHRDTPLFAVIAYGKLGGKELGYASDLDVIF
                     LYDDEDQEAPALYAKLAQRFITWMTSHTPAGILFDIDIALRPDGASGLLVSKVSAFEK
                     YQKQSAWVWEHQALTRARFCAGDAEIGQRFEAIREAVLRQERDEADLKREVLAMRRKL
                     HDAHPNRTELFDLKHDEGGMIDIEFIVQYLVLRHACKYPQLTADIGNIALLRLCGELG
                     LIDAGLALNVSDAYRRFRKLQHQMRLQGEERARVGAEKVTEEVAAVQRLWAAIFG"
     gene            complement(1943..2953)
                     /gene="gap"
                     /locus_tag="VM406_07680"
     CDS             complement(1943..2953)
                     /gene="gap"
                     /locus_tag="VM406_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HVL75881.1"
                     /translation="MTIRVAINGYGRIGRNILRAHYEGGKKNDIQIVAINDLGNAQSN
                     AHLTRYDTAHGKFPGTVEVDGDYMVVNGDKIRVFAQRDPAQIPWGELNVDVVLECTGF
                     FTSKEKASAHLKGGAKKVIISAPGGKDVDATVVFGVNQDVLKASDTVISNASCTTNCL
                     APLVKPLHDKIGLLNGLMTTVHAYTNDQVLTDVMHEDLRRARSATQSMIPTKTGAAAA
                     VGLVLPELNGKLDGFAIRVPTINVSIVDLSFIAARDTSVEEVNAIMKAAADGALKGIL
                     TYNTDPLVSVDYNHNPASSNFDATLTKVSGRLVKVSSWYDNEWGFSNRMLDTTVALMN
                     AK"
     gene            complement(3090..>3313)
                     /locus_tag="VM406_07685"
     CDS             complement(3090..>3313)
                     /locus_tag="VM406_07685"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233434.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HVL75882.1"
                     /translation="RQDAAYKASVLPKGLPRVAIEAGVTAFWHKYVGLEGAVIGIDTF
                     GESAPAGVLFKHFGFTTENVVAKVKGVLG"
BASE COUNT          579 a         1070 c         1099 g          565 t
ORIGIN      
        1 gccaggaggc gcgccacccc gagcgcagcc acaatgtcaa gcttggccgc ggcggcatcc
       61 gcgagatcga attccttgcc caggtattcc agctaatccg tggcggccgc gacaccgagc
      121 tgcgcgaccg ttccacgcgc aggacgctgg acatgctggc gcacaagcag ttgctgtcgg
      181 cagacgacgt cgcgcagctg caggccgcct acgccttcct gcgcaatctc gagcaccggc
      241 tgcagtaccg cgaggatgcg cagacccaca cactgccgcc gggcgacgag gagcggctgg
      301 ttgtcgcgca gatgatgggc tgcgcgacgg tggacgaact gctggccgaa ctcgggcgcg
      361 agcgcgccat cgttgccgcc cagttcgatg cgatgttcag cgagaaggga agcgacgacg
      421 gcgactccga cgaggccgcg catgcgccgc tctcggagac cgaaggtgtg gacagcctgg
      481 aagcgcggct gcaggactgc ggctttccgg caccgcgcga ggccgcgcag cgcctgatgg
      541 cgacaatcca gtctgccaag ctgcattcgc tgcctgcggc gagccgcacc aagctgctcg
      601 cgctgatcca caccgccctg ccgctgatcg gcgaagagcc ggcgaccgcg accgccacgc
      661 tcggccgctt gcttgatttc ctcgaggcga tcgcacgacg tgccgcctat ctcgcgctgc
      721 tgaccgagta cccgcatacg ctggagcgcg tgatccgcat gatgtgcgcg agccgctggg
      781 ccgcccagta cctgacccgg catccgatcc tgctcgatga attgctggag gatcgcacgc
      841 tgcatgcggc gcccgactgg ccggccttcg cgcaggaagt gcagcgccgg ctcgaagcgg
      901 ttgaaggcga taccgagatg cagatgaacg tgttgcgcga actgcaccat gcgcagctgt
      961 tccgcctgct cgcccaggac ctggaaggca tgctgacggt ggaaaagctc gccgaccacc
     1021 tgtccgcgct ggccgacatc ctggtgcatg caaccatcgg cgcggcgtgg aagagcattc
     1081 ccggccgcca tcgcgatacg ccgctgtttg cggtcatcgc ctacggcaag ctcggcggca
     1141 aggaactcgg ctatgcgtcg gacctcgacg tgatcttcct ctatgacgac gaggaccagg
     1201 aggcgcccgc gctgtatgca aaacttgcgc agcgcttcat cacctggatg accagccaca
     1261 cgccggccgg catcctgttc gacatcgaca tcgcactgcg cccggacggc gcgtcgggac
     1321 tgctggtatc gaaggtgtcg gcgttcgaga aataccagaa gcagtccgcc tgggtatggg
     1381 agcaccaggc gctcacgcgc gcgcgcttct gcgcgggcga tgccgagatc gggcagcgct
     1441 tcgaggcgat ccgcgaggcg gtgctgcgcc aggaacgcga cgaggcagac ctcaagcggg
     1501 aagtgctggc gatgcgcagg aagttgcacg acgcgcatcc gaaccgcacc gagctgttcg
     1561 atctgaagca cgacgaaggc gggatgatcg acatcgaatt cattgtgcaa tacctggtgc
     1621 tgcgccacgc atgcaagtac ccgcagctga ccgccgacat cggcaacatc gcgctgctca
     1681 ggctgtgcgg cgagctgggc ctgatcgacg caggacttgc gctgaacgtc tccgacgcgt
     1741 accgcaggtt ccgcaagctg cagcaccaga tgcgcctgca gggcgaggaa cgggcgcgcg
     1801 tgggcgcgga gaaggtcacc gaggaagtcg ccgcggtgca gcggctgtgg gccgcgatat
     1861 tcggctgacc ggcatcgcaa gccgatgccg aaagcaaaac gccccggcat gccggggcgt
     1921 tttgcttttg cacggtcgaa gcctacttcg cgttcatcag cgcaaccgtg gtgtccagca
     1981 tgcggttcga gaagccccac tcgttgtcgt accaggacga caccttcacc aggcggccgg
     2041 agaccttggt cagggtcgcg tcgaagttcg acgaagccgg gttgtggttg tagtcgaccg
     2101 agaccaacgg atcggtgttg taggtcagga tgcccttgag cgcgccatcg gcggctgcct
     2161 tcatgattgc gttgacttcc tcgaccgagg tgtcgcgcgc ggcgatgaag gacaggtcga
     2221 cgatcgagac gttgatggtg ggcacgcgga tcgcgaagcc gtccagcttg ccgttcagct
     2281 cgggcagcac caggccgact gccgcagcgg cgccggtctt ggtcgggatc atcgactggg
     2341 tcgcggaacg ggcgcggcgc aggtcttcgt gcatcacgtc ggtcagcacc tggtcgttgg
     2401 tgtaggcgtg cacggtggtc atcaggccgt tcaacaggcc gatcttgtca tgcagcggct
     2461 tgaccagcgg ggccaggcag ttggtggtgc acgaggcgtt cgaaatgacc gtgtcggaag
     2521 ccttcaacac gtcctgattc acgccgaaca cgacggtcgc atccacgtcc ttgccgcccg
     2581 gcgcggaaat gatcaccttc ttcgcgccgc ccttcaggtg ggccgaggcc ttttccttgc
     2641 tggtgaagaa gccggtgcac tcgagcacca cgtcgacgtt cagctcgccc cagggaatct
     2701 gggccggatc gcgctgcgcg aacacgcgga tcttgtcgcc gttgacgacc atgtaatcgc
     2761 cgtcgacttc gacggtgccc gggaacttgc cgtgcgcggt gtcgtagcgg gtcagatgcg
     2821 cgttcgactg ggcgttgccg aggtcgttga tcgcgacgat ctggatgtca ttcttcttgc
     2881 cgccttcgta gtgggcacga aggatgttgc ggccgattcg gccgtagccg ttgattgcaa
     2941 cgcggatggt catagtcttc tcctgaacga aaaatctccg aacactccct ccccctcagg
     3001 gggagggccg gggtgggggt gggtctcgct cgctctgccg cgaagaaccc atccccctcc
     3061 ccgcctctcc cgcgaaggga gaggagtcat taacccaata cgcccttcac cttggcaacg
     3121 acgttctccg tcgtgaagcc gaaatgcttg aacagcacgc cagccggcgc cgattcgccg
     3181 aacgtgtcga tgccgatcac cgcgccttcc aggccgacgt acttgtgcca gaaggccgtc
     3241 acgccggctt cgatggccac gcgcggcagg cccttcggca gcacgctggc cttgtaggcc
     3301 gcatcctggc ggt
//
LOCUS       DATLGF010000257         6778 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_117612, whole genome shotgun sequence.
ACCESSION   DATLGF010000257 DATLGF010000000
VERSION     DATLGF010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6778
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117612"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..490
                     /locus_tag="VM406_07690"
     CDS             80..490
                     /locus_tag="VM406_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVL75883.1"
                     /translation="MADDNPSIISHVSIGTNDFAAAVRFYDSVLAALDIRRVMDHPGA
                     AAYGKRFPEFWVQTPIDGYPASVGNGTHVSFVAASKQAVDAFYQAALEAGATDSGAPG
                     PRPMYGDPYYGCFVRDPDGHKIEATFWDSGGAAV"
     gene            complement(494..1978)
                     /locus_tag="VM406_07695"
     CDS             complement(494..1978)
                     /locus_tag="VM406_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HVL75884.1"
                     /translation="MPIPSLSTRARLLLSHGFLLLALALILLLALSGGPPAVTAGLAA
                     VALLAGFGAAWAAMRAVSHSFAAANDAARRLAAGDLSEPIPVHGPAEARALLRALHEA
                     GERMFAVVCSVRTGTTAVASMSAALASDNAALSARTEAQAGSLEETASSVEELTSTVK
                     QNAVHAAEANRLAGSASQAAGDGGKVVEDVVRTMGAIRDRSHKVVDIIGVIDGIAFQT
                     NILALNAAVEAARAGEQGRGFAVVAAEVRSLAHRSAAAAKEIKQLIGDSVEQVEAGSR
                     LVDQAGAAMGGIIASVERVTAIMNEISAATREQSSGIEEISRAIVQVDAGTQQNAGLV
                     EAAGRATAHLHDQAVMLTGAVAGFSLGAREFGNADDAAAMVRNAVAFCAAQGAEALID
                     EVNKLGRGRFIDRDLYISVYSGTGQVLAHGANPRLVGADGSTFKDTDGKLFVKEIVAR
                     ARQAGSGWLDYKWAHPVTKKTLVKTTCFERAGDLVVACGFYKNA"
     gene            complement(2159..2545)
                     /locus_tag="VM406_07700"
     CDS             complement(2159..2545)
                     /locus_tag="VM406_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVL75885.1"
                     /translation="MAGFCINIEEKTLSNDNFREVLFTTSRSQVVVMTLQPGEEIGQE
                     KHDTHDQFIRVEAGEGKAIMDGEEHALEDGVAIVIPAGTEHNVINTSKTEPMRLYTIY
                     APPEHPDRTVHKTKAEADEYERQHGH"
     gene            complement(2633..2863)
                     /locus_tag="VM406_07705"
     CDS             complement(2633..2863)
                     /locus_tag="VM406_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HVL75886.1"
                     /translation="MIVAARTASQVLVHMSVAFLIMYAFTGSLAFGGLAAVLEPICVV
                     GILPLHDRLWKSIEARAVKKTAALPEAAHAAQ"
     gene            complement(2961..4037)
                     /gene="ltaE"
                     /locus_tag="VM406_07710"
     CDS             complement(2961..4037)
                     /gene="ltaE"
                     /locus_tag="VM406_07710"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HVL75887.1"
                     /translation="MSDFRSDTVTRPTAAMKAAMLEAPLGDDVFGDDPTVNRLQEVAA
                     GMLGFEAALFAPSGTQTNLIALMTHCQRGDEAIVGQQWHTYRWEGGGMAVLGSIQPQP
                     LEHQPDGTLALADIEAAIKPDDPHFARTRLVVLENTTGGKVLPQHYMQDVRDLAARRG
                     LSTHIDGARLFNAAVALAPAGQALQQARALCAGYDSLSLCLSKGLGAPVGSLLLGSKD
                     FIARARRVRKMLGGGMRQAGMLAAAGLYALEHHVARLADDHANARLLAEGLGAAARGN
                     PALAGRMHVHPVQTNILFTDIDAALADPLLAHLAAHGIRVTSGYVGAGERSVRRVRWV
                     THLDVGRKDVERAVAVLQDWSEAV"
     gene            4234..5109
                     /locus_tag="VM406_07715"
     CDS             4234..5109
                     /locus_tag="VM406_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL75888.1"
                     /translation="MPTFDATEIRPTGSRRFVLEMMGFSDAEKSMLASTFRLTGRRAY
                     CYAEAASPEERVDIYLVNGDSAQALAELDAHRPSVYAPAVLVGRGGADLPWPRVERPI
                     HWMRLFEQLDAQMKTALAERDRREAAATAWDGRTFRRANDRRLPPEPVYLEPSPLESV
                     LVVDDSATVRAFMRAKLAPFRFDVDFAENGEKAIDMAQAKHYTCIFLDIMMPGMDGYQ
                     VCKRIKTSAATRESAVVMLTSKSSALDKFRGSWAGCDAYLGKPVGEDELLSTIARFLP
                     SARRIAQSVLSAGAA"
     gene            5109..5768
                     /locus_tag="VM406_07720"
     CDS             5109..5768
                     /locus_tag="VM406_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HVL75889.1"
                     /translation="MAAPRTVLLAGASGLVGGACLRALLADPEVVRIVLPVRRPLALP
                     AGAEARVEQHVVDFLRLDEYANLFEVDQILCALGTTIKAAGSQQAFREVDYAYPLEIA
                     RLGREGGAHHYLVVSAIGANPQSWFFYNRVKGEMERDLLALDYPSTTILRPSLLLGAR
                     GEFRFGEEVAKRFTFLTPGPYKPVHADQVAACMVQCARADLPGRRVISSAQIRRGECG
                     T"
     gene            complement(5987..6223)
                     /locus_tag="VM406_07725"
     CDS             complement(5987..6223)
                     /locus_tag="VM406_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75890.1"
                     /translation="MYMVYWTEVDEDKSVAHGRSFDSEDMRGAMHFMEELRKRQRAGE
                     PVCFVTMSSENPHSVGHPGVADPSPEYNWKKRRR"
     gene            complement(6295..>6778)
                     /locus_tag="VM406_07730"
     CDS             complement(6295..>6778)
                     /locus_tag="VM406_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018026941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HVL75891.1"
                     /translation="FVDAATLDIVIPLVLAGIALYFLLAPNAGAIDSRPRLAVGPYRS
                     VVVPGIGFYDGMFGPGAGSFFSMAGVALRGQNLVTATAHAKLLNFASNIAALAVFVAG
                     GKVLWTVGAVMVAGQVIGAWAGSHAMVRGGAKLIRPVIVTVCFAMLGRYLWQKGYLPF
                     "
BASE COUNT         1089 a         2297 c         2254 g         1138 t
ORIGIN      
        1 gctggccgtt tgagttgtcg caagcgccac ggaccgcgtg gcggccggaa ttgaatatga
       61 tggacggcta ggaggcgcca tggcagacga caatccgagc atcatctccc acgtatcgat
      121 cggcaccaac gacttcgcgg ccgccgtgcg gttctacgac agcgtgctgg ccgcgctgga
      181 catcaggcgt gtgatggacc acccgggcgc cgcggcatac ggcaagcggt ttcccgagtt
      241 ctgggtacag accccgatcg acggctatcc cgcctcggtg ggcaacggca cccatgtgag
      301 cttcgtcgcc gcctcgaagc aagccgtcga cgccttctat caggccgcgc tcgaagccgg
      361 cgccaccgac tccggcgcgc ccggtccgcg cccgatgtac ggcgacccgt actatggctg
      421 cttcgtgcgc gatccggacg gacacaagat cgaagcgacg ttctgggata gcggcggcgc
      481 cgcggtctga gtcctacgcg ttcttgtaga agccgcaggc gacgacgaga tcgcccgcgc
      541 gttcgaaaca ggtggtcttg accagggtct tcttcgtcac cggatgggcc cacttgtagt
      601 cgagccagcc cgagccggcc tgacgcgccc gcgcgacgat ctccttgacg aacagcttgc
      661 cgtcggtgtc cttgaacgta ctgccgtcgg cgccgaccag gcgcgggttg gccccgtggg
      721 ccagcacctg gcctgtcccg gaatagacgc tgatgtacag atcgcggtcg atgaaacggc
      781 cgcggccgag cttgttgact tcgtcgatca gcgcctcggc tccctgcgcg gcgcagaagg
      841 ccaccgcatt gcgcaccatc gccgccgcgt cgtcggcgtt gccgaactcg cgcgccccga
      901 gcgaaaagcc ggccaccgcg ccggtcagca tcaccgcctg gtcgtgcagg tgcgcggtgg
      961 cccggcccgc cgcctcgacg aggccggcat tctgctgcgt gccggcgtcg acctgcacga
     1021 tcgcgcggct gatttcctcg atgccgctgc tctgttcccg ggtcgccgcg ctgatctcgt
     1081 tcatgatcgc cgtcacccgt tcgaccgagg cgatgatccc gcccattgcc gcgcccgcct
     1141 gatcgaccag acggctgccg gcttccacct gctcgaccga atcgccgatc agctgcttga
     1201 tctccttcgc ggccgcggcg gaacggtggg ccaggctgcg cacctcggcg gcgaccactg
     1261 cgaagccgcg gccctgttcg ccggcgcgcg ccgcctccac cgccgcgttg agcgcaagga
     1321 tgttggtctg gaacgcgatg ccgtcgatca cgccgatgat gtcgaccacc ttgtgcgagc
     1381 gatcgcggat cgcgcccatc gtgcgcacca cgtcttccac caccttgccg ccgtcgccgg
     1441 ccgcctgcga cgccgatccg gccagccggt tggcctcggc cgcatgaacc gcgttctgct
     1501 tgaccgtcga tgtcagctct tcgaccgatg acgcggtttc ttccagcgaa ccagcctggg
     1561 cctcggtccg cgccgacagc gcggcgttgt cgctcgccag cgcggccgac atgctggcca
     1621 cggcggtggt gccggtgcgc acgctgcaga cgacggcgaa catgcgctcg ccggcctcgt
     1681 gcaatgcgcg aagaagcgca cgcgcctccg ccggaccgtg gaccggaatc ggttcggaga
     1741 ggtcgcccgc ggcaagccgg cgcgccgcgt cattggctgc ggcgaagctg tgggaaaccg
     1801 cgcgcatggc cgcccatgcc gcgccgaagc cggccagcag cgcgaccgct gcgagacccg
     1861 cggtgaccgc gggcgggccg ccggacagtg caagcagaag gatcagcgca agggcgagca
     1921 ggaggaaacc gtgggacagc agcagccgcg cacgggtcga gagcgagggg atgggcatag
     1981 gacctttccg cggatgtccg ccgatgcggc gcgccggccg agcttgaatg cccgctggac
     2041 gcgggcgcgc ttgccgctta ctttaggtca aaatccggga aatggcttgc tgcgcagcaa
     2101 tacgtgtgtg gtcgcagcgg gacgccgcgt ccgcccgggg agggcggcgc gggtatcatc
     2161 agtggccgtg ctgtcgctcg tactcgtcgg cttccgcctt ggtcttgtgc acggtgcgat
     2221 ccgggtgttc aggcggcgcg tagatcgtat acagccgcat cggctcggtt ttcgacgtgt
     2281 tgatcacgtt gtgttcggtg ccggccggaa tcacgatggc cacgccatcc tcgagcgcgt
     2341 gctcctcgcc gtccatgatc gccttgccct cgccggcctc gacgcggatg aactggtcgt
     2401 gggtgtcgtg cttttcctgg ccgatctctt cgccaggctg cagggtcatc acgacgacct
     2461 ggctgcgcga ggtcgtaaac agcacttcgc ggaagttgtc gttggacaac gtcttctctt
     2521 cgatgttgat gcagaaacct gccatctgtg tttctcctgt ctgatctgat ccggatgatc
     2581 atagggaacg cgcagccgga tccggttgcg ccacatcaag tatggccgga tctcactgcg
     2641 ccgcatgcgc ggcttccggc agcgccgctg tcttcttgac cgcgcgcgct tcgatggact
     2701 tccacagccg gtcgtgcagc ggaaggatgc ccacgacgca gatcggctcg agcaccgcgg
     2761 ccagtccgcc gaacgcgagc gaaccggtga acgcgtacat gatcaggaag gccacgctca
     2821 tgtgaacgag gacctggctg gcggtgcggg cggcaacgat catttgactc tccttcagtc
     2881 catctatcca tgagctgaat gatagtcatt ctcgttcgcg gcctcaaatt gattgatcct
     2941 agagaatcaa tagccgctct ctataccgct tcgctccagt cctggagcac cgccaccgcg
     3001 cgctccacgt ccttgcggcc cacgtccaga tgggtgaccc agcgcacgcg gcgcacgctg
     3061 cgttctccgg cgccgacata gccgctggtg acgcggatgc cgtgcgccgc gaggtgggcg
     3121 agcagcgggt ccgcaagcgc cgcatcgatg tcggtaaaca ggatattggt ctgcaccgga
     3181 tgtacgtgca tgcgcccggc cagcgccggg ttgccgcgtg ccgccgcccc cagcccctcg
     3241 gccagcaggc gcgcattcgc atgatcgtcc gccagccggg cgacatggtg ctcgagcgcg
     3301 tacaagcccg ccgcggccag catgccggcc tggcgcatgc cgccaccgag catcttgcgc
     3361 acgcgccgcg cgcgggcgat gaagtccttc gagcccagca gcagcgagcc caccggcgcg
     3421 cccagcccct tggacaggca cagcgacagc gagtcgtacc cggcgcacaa ggcgcgcgcc
     3481 tgctgcagcg cctgtccggc cggcgccagc gcgaccgcgg cattgaacag gcgtgcgccg
     3541 tcgatatgcg tggacaggcc gcgacgcgct gccagatcgc gcacgtcctg catgtaatgc
     3601 tgcggcagta ccttgccgcc agtcgtgttt tccagaacga ccagacgggt gcgggcaaag
     3661 tgcggatcgt ccggcttgat cgccgcctcg atgtcggcca gcgccagcgt gccgtcgggc
     3721 tggtgctcca gcggttgcgg ctggatcgat ccgagcaccg ccatgccgcc gccttcccaa
     3781 cggtaggtat gccattgctg gccgacgatc gcttcgtcgc cgcgctggca gtgcgtcatc
     3841 agggcgatca ggttggtctg ggtgccgctg ggagcgaaca gggccgcctc gaagccgagc
     3901 atcccggcgg cgacctcctg cagccggttg acggtggggt cgtcgccgaa tacgtcgtcg
     3961 cccagcgggg cttccagcat ggccgctttc atggcggcgg tggggcgggt cacggtatcg
     4021 ctgcgaaagt cgctcatggg cgttccagtg gcgggtgtcg aagccggcat tatccgggtt
     4081 ttcgaaaaag cgtgatggca actccggccc tgtccatgaa cgtaaagtta cgaacggcaa
     4141 gcttttgcgt gtgcgatact catggaagct gttattattt gagcaatacg cttgacgcgc
     4201 tcccgaaaaa ccgccctgct ccagtcacgc tccatgccta ccttcgacgc caccgaaatc
     4261 cgcccgaccg gcagccgccg cttcgtcctg gaaatgatgg gtttttccga tgccgaaaaa
     4321 tccatgctcg cgtccacgtt ccggctgacc ggccggcgcg cctactgcta cgcggaggcg
     4381 gccagtccgg aggagcgggt cgacatctac ctcgtgaacg gcgacagcgc gcaggcgctc
     4441 gccgaactcg atgcgcacag gccgagcgtg tatgcgccgg cggttctggt cgggcgagga
     4501 ggagcggacc ttccctggcc ccgggtcgaa cggccgatcc actggatgcg cctgttcgag
     4561 cagctcgatg cgcagatgaa aacggcgctg gccgagcgcg accgccgcga agccgcggcc
     4621 accgcctggg acgggcgcac cttccgccgc gcgaatgacc gcaggctgcc gccggaaccg
     4681 gtctatctcg aacccagccc gctggagtcg gtgctggtcg tcgatgacag cgcgaccgtg
     4741 cgcgccttca tgcgggccaa gctcgcgccg ttccgtttcg acgtggattt cgccgagaac
     4801 ggcgaaaagg cgatcgacat ggcgcaggcc aagcactaca cctgcatctt tctcgacatc
     4861 atgatgccgg gcatggacgg ctaccaggtg tgcaagcgca tcaagaccag cgcggccacc
     4921 cgcgagagcg cggtggtgat gctgaccagc aaatcctccg cgctcgacaa gttccgtggc
     4981 tcctgggccg gttgcgacgc ctatctcggc aagcccgtgg gcgaggatga actgctgtcg
     5041 acgattgcgc gctttctgcc gagcgcgcgg cgcatcgcgc agtcggtgtt gtccgcgggt
     5101 gcggcctgat ggccgcgccg cgcaccgtcc tgctggccgg tgccagcggt ctggtgggcg
     5161 gcgcctgcct gcgcgcgctg ctggccgatc cggaggtggt gcgcatcgtg ctgccggtgc
     5221 gtcgcccgct tgcgctgccg gcgggagccg aagccagggt cgagcagcat gtggtcgatt
     5281 ttctccggct tgacgagtac gcgaacctgt tcgaggtcga ccagatactg tgcgcgctcg
     5341 gcacgacgat caaggcggcc ggctcgcaac aggcgtttcg cgaagtcgac tacgcgtatc
     5401 cgctcgagat cgcccgtctc gggcgggagg gcggcgccca ccattacctg gtggtgagcg
     5461 ccatcggcgc aaatccgcag tcctggttct tctacaaccg ggtcaagggc gagatggagc
     5521 gcgacctgct ggcgctcgac tacccctcga ccacgatcct gcgcccctcg ctgctgctgg
     5581 gcgcgcgcgg cgaattccgc ttcggcgaag aagtcgccaa gcgtttcacg ttcctgacgc
     5641 ccggtccgta caagccggtc catgcggacc aggtcgcggc ctgcatggtg cagtgcgcgc
     5701 gcgccgacct gccgggacgg cgcgtgatct cctccgccca gatccggcgc ggggaatgcg
     5761 ggacttgaag gtttcgagtg actttccgtg gtgagagggg aataacctga atcacgcgga
     5821 acaaggcgag cacggcgaaa acctgagcaa gctgtcgtcc cgagcgcagc gagggacctc
     5881 atcgacgcat gcactggaga tccctcgctg cgctcgggat gaaagatagt cagggtgttc
     5941 gccatattcg ccttggcgaa acagttgaag ccggtgaaac accagcctag cgccgccgct
     6001 tcttccagtt gtactccgga ctcgggtcgg ccacgccggg atggccgacg gaatgcgggt
     6061 tttccgatga catggtgacg aagcacaccg gttcccccgc gcgctgccgc ttgcgcaatt
     6121 cctccatgaa gtgcatcgcg ccgcgcatgt cttccgaatc gaaggaacgg ccatgcgcga
     6181 cgctcttgtc ttcatccacc tcggtccagt acaccatgta catgcgcgtc tcccgttgcg
     6241 aaattggatg ccgggcgcgc cggccggttc agaacggcag gtagcccttc tggctcagaa
     6301 cggcaggtag cccttctgcc acaggtagcg gccgagcatc gcgaagcaca cggtcacgat
     6361 caccgggcgg atcagcttcg cgccgccgcg caccatcgca tgcgaaccgg cccaggcgcc
     6421 gatgacctgg ccggccacca tcaccgcgcc gacggtccac agcaccttgc cgccggcgac
     6481 aaagaccgcg agcgcggcga tgttgctggc gaagttgagc agcttcgcat gggcggtggc
     6541 cgtgacgagg ttctgaccgc gcagcgccac gccggccatc gaaaagaacg agccggcgcc
     6601 ggggccgaac atgccgtcgt aaaagccgat gcccggcacc accacgctgc gatacgggcc
     6661 gaccgccagg cgcggccggc tgtcgatcgc gccggcgttc ggcgcgagca ggaagtacag
     6721 cgcgatgccg gccagcacca gcgggatgac gatgtcgagc gtggccgcat cgacgaac
//
LOCUS       DATLGF010000258         4352 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_5478,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000258 DATLGF010000000
VERSION     DATLGF010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5478"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VM406_07735"
     CDS             complement(<1..667)
                     /locus_tag="VM406_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVL75892.1"
                     /translation="MAKQYDLVIVGAGTVATVAAMRVRAADWRVAVVDFRPYGGTCAL
                     RGCDPKKMMVSAATAYDYACRMREHGVVGELHIAWPELMAFKRGFTDPVPEKMEHRYA
                     RAGIDTYHGTARFTGMNTLDVNGQALEAKHVLLATGAEPVKLGIPGEQHLVDNEAFLA
                     LEKLPPRIVLVGGGYIAAEFSHLAARAGAQVTILQHGERMLKHFDPDLVGWLMESFAS
                     LGID"
     gene            complement(719..2401)
                     /gene="merA"
                     /locus_tag="VM406_07740"
     CDS             complement(719..2401)
                     /gene="merA"
                     /locus_tag="VM406_07740"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016152 - mercury (II) reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HVL75893.1"
                     /translation="MTYLKISGMTCEGCAVHLKEALERVPGVESAAVSYPKGTAEVSV
                     ALGTTPDQLTDAVAEAGYGAELTDEPRTRSPEHSSDKRSADRNSSTEQGGEAGLHVAI
                     IGSGGAAMAAALKAVERGARVTLIERGVIGGTCVNVGCVPSKIMIRAAHIAHLRRKSA
                     FDGGISPAQPAIDPVRLLAQQQARVEELRHAKYEGILAENPTITVLRGRARFKNRQSL
                     LVQMTEGGEQVVAFDRCLVATGAHAAIPPLPGLKETPYWTSTEALASDTIPPRLAVIG
                     SSVVAVELAQAFARLGSKVTLLARSMLFSREDLAIGEALTVAFRNEGIEVQEHTRVTR
                     VDHANGEFRLATESGELRADKLLVATGRVPNTEGLALESAGVATNAHGAIAIDNGMRT
                     SVPNVFAAGDCTDRPQFVYVAAAAGGRAAINMTGGEVTLDLTAMPAVVFTEPQVATVG
                     LSEARARLEGIETDSRTLSLDNVPRALVNFDTHGFIKLVAEAGSGRLLGVQAVASEAG
                     ELIQAAALAIKAGMTVRDLADQLFPYLTMVEGLKLAAQTFTKDVKLLSCCAG"
     gene            complement(2465..2743)
                     /gene="merP"
                     /locus_tag="VM406_07745"
     CDS             complement(2465..2743)
                     /gene="merP"
                     /locus_tag="VM406_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003116826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance system periplasmic binding
                     protein MerP"
                     /protein_id="HVL75894.1"
                     /translation="MKKLWALSVLATLIAAPVWAATKTVALAVPGMTCAACPFTVKAA
                     LSKVGGVSKVDVSHEKREAVVVYDDSKTSPEALSKASANVGYPASVKQ"
     gene            complement(2758..3129)
                     /gene="merT"
                     /locus_tag="VM406_07750"
     CDS             complement(2758..3129)
                     /gene="merT"
                     /locus_tag="VM406_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric ion transporter MerT"
                     /protein_id="HVL75895.1"
                     /translation="MANTSNTRRGHGSGRDSLFAGGLAAVLASTCCLGPLVLVALGFS
                     GAWIGSLTVLEPYRPVFIGIALVALFFAWRRIFRPAEACKPGEVCAVPQVRGTYKLIF
                     WMVAALVLVALAFPYILPLFY"
     gene            3199..3609
                     /gene="merR"
                     /locus_tag="VM406_07755"
     CDS             3199..3609
                     /gene="merR"
                     /locus_tag="VM406_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HVL75896.1"
                     /translation="MEYRLENLTIGAFAAAANVNVETIRFYQRKGLLQRPTKPLGGIR
                     RYAQADVERVRFVKSAQRLGFSLDEIGELLRLEDGMHCEEASTLAEHKLTEIHKKVED
                     LTRMKAALSALVGACHAKKDEVSCPLISALHGYP"
     gene            3794..>4352
                     /locus_tag="VM406_07760"
     CDS             3794..>4352
                     /locus_tag="VM406_07760"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HVL75897.1"
                     /translation="MIEPIGHALYVGAGLFWQAFWALAFGYAFSSLIQVLVPREVIAK
                     YLGRGGIKQIGLAMVMGPASSSCSFAALSAGRALLQKGAAPTPMLAFLFGATNLSPQV
                     AALAWIFLGWQFALALVLGSVVHVAIMAVVVRFTLSQNRVERARKDAEMAGKKDGGMS
                     DPAEGLSGTWRDKIKRSDVWHRIGFT"
BASE COUNT          834 a         1373 c         1294 g          851 t
ORIGIN      
        1 catcgatgcc gagcgaagca aacgactcca tcagccagcc gacaaggtcg ggatcgaagt
       61 gcttcagcat gcgttcaccg tgctgcagga tggtgacctg tgcaccggcg cgagctgcga
      121 gatgggagaa ctcggctgcg atgtaaccgc cgccgacaag cacgatgcgc ggcggcagtt
      181 tctcaagcgc gagaaaggct tcgttgtcga cgagatgctg ttcgccgggt atgcccagct
      241 tgacgggctc agcccccgtg gcgagcaata catgcttggc ctcgagtgcc tggccattga
      301 cgtcaagcgt gttcatcccg gtaaaccgtg ccgttccatg atacgtgtcg atccccgccc
      361 ttgcgtagcg atgttccatc ttctctggga ctggatcggt aaaaccgcgc ttgaaggcca
      421 tcaattccgg ccacgcgata tgcagctcgc cgacgacgcc atgttcacgc atgcggcatg
      481 catagtcata tgcggtggca gcagacacca tcatcttctt cggatcgcac ccccgcaaag
      541 cacacgtgcc gccgtaaggc cggaaatcga cgaccgccac gcgccagtcc gccgcgcgca
      601 cccgcatcgc ggcaaccgtg gcgactgtgc cggcgccgac aatcaccaaa tcatactgtt
      661 tggccatctt tgctctcctg acgtatctac acttatcgcc tcaccgcgag atgccccctt
      721 atcctgcgca gcaggacagc agcttgacgt ccttggtaaa ggtctgggcg gcgagtttga
      781 gcccttccac catggtgaga tacgggaaca actggtcggc caagtcgcgc accgtcatgc
      841 ctgccttgat ggcaagcgcc gccgcctgga tgagttcgcc agcttcggaa gcaaccgcct
      901 gcaccccaag cagccggccc gaaccggctt cggcaaccag cttgatgaag ccgtgcgtat
      961 caaagttgac cagggcacgc ggcacgttgt cgagcgacag cgtgcggctg tcggtttcga
     1021 tgccctccaa gcgcgcccgg gcttcgctga ggccaacggt cgccacctgg ggctcggtga
     1081 acaccacggc cggcatcgcc gtcaagtcaa gtgtgacctc gccgcctgtc atattgatcg
     1141 ccgcgcgccc gcccgctgca gccgccacat agacgaactg cggccggtcc gtacaatcac
     1201 ctgcggcaaa cacgttcggc acactggtgc gcatcccgtt gtcgatcgcg atcgcgccat
     1261 gcgcgttggt cgccacgcct gccgattcca gtgcgagacc ttctgtgttt ggaacccgtc
     1321 cggtggcgac gagcagcttg tcggcccgca gttcgccgct ttctgtggca agccggaact
     1381 cgccatttgc gtgatcgacc ctggtaacac gcgtatgctc ctgtacctcg atgccctcgt
     1441 ttcggaacgc aaccgtcagt gcctcgccga ttgccagatc ttcccgcgag aacagcatgc
     1501 tgcgagcgag aagcgtgact ttgctgccga gccgcgcgaa ggcctgggct agttccaccg
     1561 ccacgaccga cgagccgatc acagccagcc gcggtgggat tgtgtcgctc gccaatgctt
     1621 cggtggaggt ccagtacggc gtctctttca aacccggcag cggcgggatg gctgcgtgcg
     1681 ctccggttgc aaccaggcac cgatcgaatg ccactacttg ctcgcctcct tccgtcatct
     1741 ggacgagaag gctttgccga ttcttgaagc gagcgcggcc gcgcagcaca gtaatagtag
     1801 ggttttcagc gaggatgcct tcgtacttgg catgccgcag ctcctcgacc cgtgcctgct
     1861 gctgggcgag caggcggaca ggatcgatcg cgggctgtgc cggggagatc ccgccatcga
     1921 acgcgctctt acgacgcagg tgcgcgatat gcgcagcgcg aatcatgatc ttcgacggca
     1981 cgcagcccac gttgacgcag gtgccgccaa tcacaccgcg ctcgatcagg gtgacgcgcg
     2041 cgccgcgctc gacagccttg agcgccgcgg ccatcgcagc acccccgctg ccgatgatag
     2101 ctacgtgcag gccggcctcg ccgccctgtt cggtactgct attccgatcg gcactccgct
     2161 tgtcagagga gtgctcgggc gacctagtgc gtggttcatc ggtaagctcg gcgccatacc
     2221 ccgcctcggc cacagcgtcc gtcaattggt caggcgtagt accgagggct acagatactt
     2281 cggccgtccc ctttggatag gacactgcag cagactctac ccccggcact ctttccaatg
     2341 cttccttgag gtgcaccgcg cagccttcgc atgtcatgcc cgaaatcttc aagtacgtca
     2401 tcaatcactc cttgtgggcg tcacgcatgc acgcacgcca gcatgcaagt tggcaaagac
     2461 actgctactg tttgacgctt gccggataac ccacgttcgc gctcgccttg ctcagcgcct
     2521 cagggctggt cttgctgtca tcgtagacga cgacagcttc gcgcttttcg tggctcacat
     2581 cgaccttgct cacgccacca accttcgaga gcgccgcctt cacggtgaat ggacaagccg
     2641 cacaggtcat gccgggaacg gccagggcga cggtcttcgt tgccgcccag acgggggcag
     2701 cgatgagggt ggcgagtacg gacagtgccc acagtttctt catgatgaac tccggggtta
     2761 atagaacaag ggaaggatgt acgggaaggc aagcgcgacc agtaccaacg cggcgaccat
     2821 ccagaaaatg agcttgtagg tgccgcgcac ctgtgggacc gcacatacct cgcctggttt
     2881 gcatgcctcc gccggtcgga aaatgcgccg ccaggcaaag aacagggcca ccagcgcaat
     2941 gccgatgaag accgggcggt atggctccag cacagtcagg cttccgatcc atgcgccgct
     3001 gaaacccagt gccaccagca ccagcggtcc aagacagcac gtggaggcga ggacggctgc
     3061 caagccgccg gcgaaaaggg agtcacggcc cgagccatgt ccacgtctcg tgttactcgt
     3121 gttagccata cccattctct tttcgatttt gaccgaatga cttaacctac tcccgtagtc
     3181 aagtacggag tcaagcgtat ggaataccga ctggaaaacc tgaccatcgg cgcctttgcc
     3241 gcagccgcaa atgtgaacgt ggagacaatt cgtttctatc aacgcaaggg actcttgcag
     3301 cggcccacaa aacccctcgg cggcattcga cgctacgccc aggccgatgt agagcgggtg
     3361 agatttgtga agtcagccca gcggctcggt ttcagtctgg acgaaatcgg cgaactgctg
     3421 cgtttggaag acgggatgca ctgcgaagag gcaagtacgc tggctgagca caagctcacg
     3481 gagatacata agaaggtgga ggatctcacg cggatgaagg cggcactgtc agccttggtg
     3541 ggcgcctgtc acgcaaagaa agacgaagtc tcgtgcccgt taatctcggc gttgcacggt
     3601 tatccgtaag ggtgagaggt cttggtcagc gcctcggaag ccatgttgct cccgctgatt
     3661 tgcgtcaact tgttctcggg agtgggcgac ttgttgatcc agtccaagcg cgtatgagag
     3721 gctcttttct gcactcccgc tctgtcccca aggtctctcc ggggcctatc cggagcccca
     3781 ctcaggacgg gctgtgatcg aaccaatcgg acatgcgcta tatgtcggcg ccgggctttt
     3841 ctggcaggcg ttctgggcct tggcgttcgg ctatgctttc tcctcactga tccaggttct
     3901 tgtgccgcgg gaggtcattg ccaagtacct cggccgcggc ggaatcaagc aaatcggcct
     3961 ggcgatggtg atggggccag cttcatcgtc gtgctcattc gccgcgctct ccgcaggccg
     4021 cgctctgctc cagaagggag ctgcgcctac cccgatgcta gcgtttctgt tcggcgcaac
     4081 gaatctgtcg ccacaagttg ccgcgctcgc ctggattttt cttggttggc aatttgcgct
     4141 tgcgctagtg cttggctcgg tcgtacatgt cgccatcatg gcagtcgttg tgcgattcac
     4201 gctttctcaa aacagagtcg agcgtgcgcg caaggacgcc gaaatggccg gcaagaagga
     4261 cggcgggatg agcgatccag cagaaggcct atcgggcaca tggcgcgaca agatcaagcg
     4321 cagcgacgtg tggcatcgta tcggatttac ct
//
LOCUS       DATLGF010000259        13993 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_168093, whole genome shotgun sequence.
ACCESSION   DATLGF010000259 DATLGF010000000
VERSION     DATLGF010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 13993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13993
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168093"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="VM406_07765"
     CDS             complement(<1..1050)
                     /locus_tag="VM406_07765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HVL75898.1"
                     /translation="MSFQQGLSGLNAAAKSLETIGNNVANANTVGFKGSQAQFADVFA
                     SSLTGATATQIGIGTKLAQVSQQFTQGNITASSNPLDVAINGGGFFRLSTNGTITYSR
                     NGQFQLDSGGYIVNSNGARLTGYVADARGVLSTGAPSELNIRTADLAPRATTGVTAAL
                     NLDSRMSPPIVPVFDMNEPTSYNSSSSVTVFDSLGNAHVLQTYYVKTDPTPPSTGGTW
                     QVYASSNGRPLGAVDAVPATTAGNVVALGAPTSLSAIPAGTFSINGVAIGAVAAGADA
                     AEQGANVAAAINAANIPGVTASADGAGVVTITNAGNTTISMNGAASSPTAASDAHADL
                     LAATGFGAAQVGTQAV"
     gene            complement(1082..1747)
                     /locus_tag="VM406_07770"
     CDS             complement(1082..1747)
                     /locus_tag="VM406_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HVL75899.1"
                     /translation="MTSVQTVSPDLLGAMNASRKSEASIAEEAQNRFMTLLVTQMRNQ
                     DPLNPLDNAQVTSQLAQLSTVTGIDKLNLTLQSLQASYMASQSMQAANMIGHGVLVPG
                     DTMLLVEGKGLMGVELPEAADSVVVTIRDASGRAVHALDLGAQKAGNVPFGWDGVKQD
                     GTQAGDGVYTFEVTAMRGEQKVEAKALAFGIVSSVSTGAKGVTLNVSGLGSLQMSDVR
                     EIL"
     gene            complement(1760..2164)
                     /gene="flgC"
                     /locus_tag="VM406_07775"
     CDS             complement(1760..2164)
                     /gene="flgC"
                     /locus_tag="VM406_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HVL75900.1"
                     /translation="MSLFNIFNVAGSAMSAQGQRLNTVASNIANADSTASADGKVYRS
                     KQVVFQAVPTAAPASAGVKVQQVVESQAPLRVVYDPKHPQANEEGYVTMPNVNVVEEM
                     VNMISASRSYQTNVETMNAAKTMLLKTLTLGQ"
     gene            complement(2185..2598)
                     /gene="flgB"
                     /locus_tag="VM406_07780"
     CDS             complement(2185..2598)
                     /gene="flgB"
                     /locus_tag="VM406_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HVL75901.1"
                     /translation="MIGKLDETLRFHQTVLALRGERQQVLASNIANADTPNYKARDID
                     FNSALKGALAKNGTPAPALVKTSGAHLSGVSAGVTATGAPLLYRTPAQGSVDGNTVDM
                     DAERNQFLDNALRYEASVTFAGGQLKKLMTAIQGQ"
     gene            2775..3485
                     /gene="flgA"
                     /locus_tag="VM406_07785"
     CDS             2775..3485
                     /gene="flgA"
                     /locus_tag="VM406_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HVL75902.1"
                     /translation="MRLPSRFLLAASWLVAACLGAAAAQASAPRHDPAQIRLQVEHYL
                     RTQTNGLPGEVNITVGQVDTRIALPMCAAPEPFLPNGARLWGKTSVGVRCSAPLPWTI
                     YVPATVQVLGEYLVAAAPLASGQTLGPNDVAKMTGDLTALPNGVVTDPSLAVGRTLTM
                     SIAAGAPLRGDALRAPQAVQQGQTVRLVSIGPGFRVSAEGRALNNAADGQIAQARTAS
                     GQVVSGIARAGGVVEVSY"
     gene            3589..3903
                     /gene="flgM"
                     /locus_tag="VM406_07790"
     CDS             3589..3903
                     /gene="flgM"
                     /locus_tag="VM406_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HVL75903.1"
                     /translation="MKIDDSVKKTTGLSVGATPARAGNGADKAAASGAATTTPSQSDS
                     VHLSTKLQSLAQTGGNAVFDAKKVEEIKAAIAEGRFQVDPEKVANGLLDTVTDLIHAR
                     KS"
     gene            3909..4385
                     /locus_tag="VM406_07795"
     CDS             3909..4385
                     /locus_tag="VM406_07795"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HVL75904.1"
                     /translation="MQAFASTPAQTLSEELAAARALLKLLNDEQELLVQADVDGLNRA
                     LEEKGSLVAKMGELAMRRYRHLTDAGFDGSEAGMQSWLNSRKPAGTAQQDWAALLELA
                     RQGKELNRTNGMLIGQHMARTQSALNVLQGAPEGGQMYGPNGQATTAAGGRRLVVG"
     gene            complement(4663..5454)
                     /gene="motD"
                     /locus_tag="VM406_07800"
     CDS             complement(4663..5454)
                     /gene="motD"
                     /locus_tag="VM406_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotD"
                     /protein_id="HVL75905.1"
                     /translation="MARRRRRHEEEHENHERWLVSYADFITLLFAFFVVMYALSSVNE
                     GKYRILSNSLINAFGRTQAGPVQTGPIPAESVVPVAVPAPRPRPVPAPADAALRRETE
                     RLTGMARDIQRVLAPLVEQGKVRVTQSPRGVTVEINASVLFAPGDARLAPASVHAMQA
                     VAAVLKDDDHAIQVEGHTDSVPISNVAFPSNWELSAVRASSVVRLFVDAGVAQTRLTA
                     VGYGETRPVDSNETADGRQRNRRVAVLILSHLPETATEVPLGGGA"
     gene            complement(5459..6199)
                     /locus_tag="VM406_07805"
     CDS             complement(5459..6199)
                     /locus_tag="VM406_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HVL75906.1"
                     /translation="MDWASLIGLALGLFGILAGQALEGGHIGSLVQPAAFTIVVIGTF
                     GAVLLQSGMDSFMRGMRMLGWIFRRPAGIDPGLSNQVSTWSSTARREGLLSLERYVDA
                     IEDGFTAKGLRLIVDGIDPYKIREILEADISAYERRERQAVKIWEAAGGYAPTIGILG
                     AVLGLIHVMENLSDPSRLGGGIAVAFVATVYGVGFANLVFLPIANKLKGLVADEVERR
                     EMLTDVFCSVANGDNARVVTERLAAYGR"
     gene            complement(6181..6912)
                     /locus_tag="VM406_07810"
     CDS             complement(6181..6912)
                     /locus_tag="VM406_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HVL75907.1"
                     /translation="MYTVSGKADKNDLLETHVPLVKKLAHQMKAKLPPSVEVDDLIQA
                     GLIGLLDAVSRYEETHGAQFETYAVQRIRGAMLDELRSSDWLPRTVRQNMRKIETAMN
                     KVQQRMGRPPREAEIAGELKVSLEEYQAMLDEGSGHQLVYYEDFHDRDENDHFLDRFI
                     MDEASDPLQALTNAGFRQAVIKAIEALPEREKILMGLYYEQEMNLKEIGAVMGVSESR
                     VSQLHSQAIARMRNRLREQAWTGLV"
     gene            complement(6937..7731)
                     /locus_tag="VM406_07815"
     CDS             complement(6937..7731)
                     /locus_tag="VM406_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiactivator of flagellar biosynthesis FleN
                     protein"
                     /protein_id="HVL75908.1"
                     /translation="MASFEFDQAEGLRRMLSGPKPRVLTLLSAAPREEKGAMLINLGA
                     SLVQAGRDVLLLDACTESQGISPSLDAVRAASLVQVARGERGIEEAVQPLPQGFGVAA
                     VRRPAQPMCGTQELDDAFGLLARRADVVLVDAELDRNDAFPIPALAASEIVVQVVNTP
                     DSIKSAYAIIKRLNGQLGRRPFSLVVTGASDKEAQLVYQNMAQAANRYLAVRLNSMGS
                     VPPDEHLKRAMRLGRSVVEAFPLAGASVAFRRLAGKVVLFDAAAAG"
     gene            complement(7724..9052)
                     /gene="flhF"
                     /locus_tag="VM406_07820"
     CDS             complement(7724..9052)
                     /gene="flhF"
                     /locus_tag="VM406_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HVL75909.1"
                     /translation="MNVRKFSAHSSREAWRQVREALGPDAVILSNRSINGTIEILALA
                     PEDMSALGEPVIEKPAVSEATLGALGARRVAEQAPAAPLPPVGAPRAIEETDQASGLA
                     GALAASREGPRQSRTTEQGNELAALMSELRSMRGMLESQLAEIAWSSQQKREPMKAAL
                     MREMLSAGFSASLARYLTQNLPAGEKDGMAWTKAILARNLSVIGNENEILEKGGVYAL
                     VGPTGVGKTTTTAKLAARCVMRHGAGKLALITTDGYRIGGYEQLRIYGKILGVMVHSV
                     KDETDLRIALDELKNKHMVLIDTVGMGQRDKMVAEQIAMLSGSGAKVKRLLCMSATST
                     GETLNEVVRSYQGDGLAGCIMTKLDEAATVGNVLDVIIRHKLNLYYVANGQRVPEDLH
                     VANQHYLVDRAFKLKRETAPYLYKDDELPLVIANAAHAMHDKSLREVSLG"
     gene            complement(9049..11148)
                     /gene="flhA"
                     /locus_tag="VM406_07825"
     CDS             complement(9049..11148)
                     /gene="flhA"
                     /locus_tag="VM406_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HVL75910.1"
                     /translation="MNSLRLPAFLSGANSRAMAAPILIVMILAMMVLPLPPFALDILF
                     SFNIALAIIVLLTSLYTVRPLDFMVFPTVLLVSTMLRLSLNVASTRVVLTEGHTGPDA
                     AGKVIEAFGHFLIGGNYTVGIVVFIILTIINFVVVTKGAGRIAEVGARFALDAMPGKQ
                     MAIDADLNAGMIGEQEARARRKEVAQEAEFYGAMDGASKYVRGDAIAGILVTVINIFG
                     GLIVGMAQHDLGFDAAMRNYTLLAIGDGLVAQIPSLIISTAAGVVVSRVASEHDIGGQ
                     LVGQLFARPQVMYITAAIIGGMGMIPGMPNLPFLLLGATLAGCGYLLAQRARQEAEKP
                     EDDQKNAAAAASALPESEEATWEDIVPVDTLGLEVGYRLIPLVDKGQNGELLRRIKGI
                     RKKFAQEVGFLSPPVHIRDNLELRPTAYRFTLKGVEVGSGEAYVGQFLAINPGMVHGA
                     LPGNATTDPAFGLPATWIDGALREQAQAMGYTVVDAGTVVATHLNHLITTHAAELLGR
                     QEVQQLLDHLAKTSAKLVEDLVPKVIPLATLQKVLQNLLSEGVHIRDMRTIIETVSEY
                     APQVQDPNELTALVRVALGRAIVQQIFPSATELSVITLDSRLERVLMQALHSGGADGA
                     GIEPGLADTIAAQTEQAARQQEQMGLPPVLLVPGPLRPLLSRFLRRTLPQLKVLSHQE
                     LPDTKTIRVTSLVGGQA"
     gene            complement(11145..12299)
                     /gene="flhB"
                     /locus_tag="VM406_07830"
     CDS             complement(11145..12299)
                     /gene="flhB"
                     /locus_tag="VM406_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HVL75911.1"
                     /translation="MAEDSDLEKTEPASPRKLEQAREEGDVPRSKELATCTILLGGGI
                     AFWLAGEGLLRRLNGMLASGLSFERAHAFDFNLLLQHLSSGLFDVAIGLAPIGAILIL
                     VALASPLLIGGWLFSGKALQPKFSRMNPIKGIGNMFSMRALVELVKAIGKAVLVGVIA
                     WLVISTQIESMLALAVEPLQSAGAHLGTLLLTGFIAIVAALVIIAAIDAPYQMFHYAN
                     KLKMTREEVRKESKETDGDPQVKARIRQTQREMARRRMMSEVPTADVVVTNPSHYAVA
                     LKYTEGKMKAPKVVAKGADEVAAKIRELAREHKVPLLEAPPLARALYRHAELGDEIPS
                     ALYTAVAEVLAYVFQLRAYAQHGGVPPQEPQDLDVPRELDPHNAAVKGAQ"
     gene            complement(12434..13642)
                     /locus_tag="VM406_07835"
     CDS             complement(12434..13642)
                     /locus_tag="VM406_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HVL75912.1"
                     /translation="MPDSHFIALEPLLNNQERVLGYELRWHGAADTAPDDNAALELVR
                     CVHEHLRDDETASLLGEQRVFVEVTPALLEPSVLALLPAQGTVLILRGDALASEETIA
                     AVAKARAQGYAFSVRDANPAALDKQLLAQLSYAELRADRPDFLAQLQQWRALGPAALQ
                     PVARGVTDWERFDACAAQNVFSFAGGLYLTPRPGARARDLNPTQTLILQLMEMVQKNA
                     DVKQLESVLKRDAALSYKLLRYINSAGFGLGCEVQSLKHAVTMLGYAPLYRWLGLLLA
                     TATTSGYSPVLMQTAVIRGRFAELLGAGFLPKQEAENLFVAGMFSLLDKLLGVPMEMV
                     LEKIQLSEPITQALLTREGIYGPFLAMAEACESDESDLDGMATSLCVEASDVNRAQLS
                     ALAWTRTLQL"
     gene            complement(13687..>13993)
                     /locus_tag="VM406_07840"
     CDS             complement(13687..>13993)
                     /locus_tag="VM406_07840"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HVL75913.1"
                     /translation="RTAAFLDGARADALATRQQLLDIMMAQDFQDLTGQVIRRVTELA
                     HGIEQQLLQLLIDYAPPEVRREAGNGLLNGPQINPTGNADVVSSQSQVDDLLDSLGF"
BASE COUNT         2296 a         4735 c         4504 g         2458 t
ORIGIN      
        1 gaccgcctgc gtgccgacct gggcagcgcc gaaaccggtc gcggccagca ggtccgcgtg
       61 cgcatcggac gccgccgtgg gcgagctggc agcgccgttc atcgagatcg tggtgttgcc
      121 ggcgttggtg atggtgacga caccggcccc gtcggcggaa gcggtcacgc cgggaatgtt
      181 cgcggcattg atcgcggcag ccacgttggc cccctgctcg gccgcatcgg cgccggctgc
      241 aacggcgccg atggccacgc cgttgatcga gaaggtgccg gcagggatgg cggacagcga
      301 ggtcggcgca cccagcgcca ccacgttgcc ggccgtcgtc gccgggaccg cgtcgaccgc
      361 gccgagcggg cgtccgttgg acgacgcgta gacctgccag gtgccgccgg tcgaaggcgg
      421 cgtcggatcg gtcttcacat agtaggtctg cagcacatgt gcgttgccca ggctgtcgaa
      481 cactgtcacc gaggacgagc tgttgtagct ggtcggctcg ttcatgtcga acaccggcac
      541 gataggcgga ctcatgcgcg aatcgaggtt gagcgcggcg gtcacgccgg tggtcgcgcg
      601 cggcgcgagg tcggcggtgc ggatgttcag ctcggacggc gcgccggtcg acagtacgcc
      661 gcgcgcatcg gccacatagc cggtcaggcg cgcgccgttg gaattgacga tgtaaccgcc
      721 gctgtcgagc tggaactggc cgttgcggct gtaggtgatc gtgccgttgg tggacaggcg
      781 aaagaagccg ccgccgttga tcgccacatc cagcggattc gacgacgcgg tgatattgcc
      841 ctgagtgaac tgctgcgata cctgggccag cttggtgccg atgccgatct gggtcgcggt
      901 tgcgccggtc agcgaactgg cgaacacgtc cgcgaactgc gcctgcgaac ccttgaagcc
      961 cacggtgttt gcgttggcga cgttgttgcc gatcgtctcg aggctcttgg ccgccgcgtt
     1021 caggccgctc aaaccctgtt gaaagctcat ctttttctcc ttgttccgtc ttcgctcgcg
     1081 ctcaaagaat ttcgcgcacg tcgctcatct gcaggctgcc caggccggac acgttcaggg
     1141 tcacgccctt ggcgccggtg ctcacgctgg atacgatgcc gaatgccagc gccttcgctt
     1201 ccaccttctg ctcgccgcgc atggctgtca cctcgaaggt gtagacgcca tcgccggcct
     1261 gtgttccatc ctgcttcacg ccatcccagc cgaacggcac gttgccggcc ttctgcgcac
     1321 cgagatcgag cgcatggacc gcgcggccgg atgcgtcgcg gatcgtgacg accacgctat
     1381 cggccgcttc cggcagttcc acgcccatca gccccttgcc ttcgaccaga agcatggtgt
     1441 cgcccggcac cagcacgccg tggccgatca tgttcgccgc ctgcatcgac tggctggcca
     1501 tgtagctggc ctgcagcgat tgcagtgtga ggttgagctt gtcgatgccg gtcaccgtcg
     1561 acagttgcgc gagctggctc gtcacctgcg cgttgtccag cggattgagc ggatcctggt
     1621 tgcgcatctg cgtcaccagc agcgtcatga aacggttctg cgcttcctcg gcgatcgatg
     1681 cctcggattt gcgcgacgcg ttcatcgcac cgaggaggtc cggcgaaacg gtctgcacgc
     1741 tagtcattca gatccccttt cactgaccca gcgtcagggt cttgagcagc atggtcttgg
     1801 ctgcgttcat cgtttcgaca ttggtctgat aggagcgcga cgccgaaatc atgttgacca
     1861 tctcctcgac cacgttcacg ttcggcatcg tcacatagcc ctcttcgttg gcctgcggat
     1921 gcttggggtc gtacacgacg cgcagcgggg cctggctttc caccacctgc tgcaccttga
     1981 cgcctgcgga agcaggcgcg gccgtcggca ccgcctggaa caccacctgc ttggagcggt
     2041 agaccttgcc gtcggcactg gcggtgctgt cggcattggc gatattgctg gccacggtgt
     2101 tgagccgctg gccctgggcg ctcatcgccg agccggcgac attgaagata ttgaacagcg
     2161 acatgaccga tctccttgtt tcccttactg accctggatc gccgtcatca gcttcttcag
     2221 ctggccgccg gcgaacgtga cgctggcctc gtaacgcaat gcgttgtcga ggaactggtt
     2281 gcgctccgca tccatgtcga cggtattgcc gtccacgctg ccctgcgccg gcgtgcggta
     2341 cagcagcggc gcgccggttg ccgtgacccc ggccgacaca ccggacaggt gcgcaccgga
     2401 ggtcttgacc agagcgggcg cgggcgtgcc gttcttcgcc agcgcgcctt tgagcgccga
     2461 gttgaaatcg atgtcgcgcg ccttgtagtt gggcgtgtcg gcattggcta tgttcgaagc
     2521 cagtacctgc tggcgctcgc ccctcagggc cagcaccgtc tgatgaaagc gcagcgtttc
     2581 gtccagtttt ccgatcatga ctgcctccgg caccccttcg gcacgaccgc aggggggatt
     2641 gatgtagcgt cgataatacg gatgcaaaag tcacatccat cgggcaataa ggcttcgaaa
     2701 cccgatcctt ttcctccttt tgaaagcggg cctcgcgcgt accattcagc gtggaatcat
     2761 tgtcatcacc tgccatgcgc ctgccctccc gctttctgct tgccgccagc tggctcgttg
     2821 ccgcctgcct cggtgccgcc gcggcccaag ccagcgcgcc gcgccatgat ccggcacaga
     2881 tccgcctgca ggtcgagcac tatctgcgca cgcagacgaa cgggctgccc ggcgaggtga
     2941 acatcacggt cggccaggtc gacacccgca tcgcgctgcc gatgtgcgcg gcgccggaac
     3001 ccttcctgcc gaatggcgcg cgtctgtggg gcaagacttc ggtcggcgtg cgctgcagcg
     3061 cacccttgcc ctggaccatc tatgtgccgg ccaccgtcca ggtgctcggc gaatacctgg
     3121 tcgccgccgc cccgcttgcc agcggacaga cgctggggcc gaacgacgtg gcaaagatga
     3181 ccggcgatct gaccgcgttg ccgaacggcg tggtgacgga cccgagcctc gccgtcggcc
     3241 gcacgctgac gatgtcgatt gccgccggcg ccccgctgcg gggcgatgca ttacgcgcgc
     3301 cgcaggcggt gcagcagggc cagacggtgc gcctggtctc gatcggcccg ggtttcaggg
     3361 tttctgcgga aggccgggca ctgaacaatg ccgccgacgg tcagattgcg caggcgcgca
     3421 ccgcctccgg gcaggtggtc agcggcatag cacgtgccgg cggcgtcgtg gaggtcagct
     3481 actgaacttg caattatttg caatttctca agccctccgc cctaaagttc ccgggatttg
     3541 ggtcgataaa cgggatgaca gaatcccact cccagcaagg atacgatcgt gaaaatcgat
     3601 gactccgtaa agaaaacaac aggcctctcc gtcggcgcca cgccggcgcg cgccggcaac
     3661 ggtgcggaca aggctgccgc cagcggcgcc gccacgacca ccccgagcca gtccgacagc
     3721 gtgcacctgt cgacgaagct gcagtcgctt gcccagaccg gcggcaacgc agtgttcgac
     3781 gcgaagaagg tggaagaaat caaggcggcc atcgccgagg gccgtttcca ggtcgatccg
     3841 gaaaaagtcg ccaacggcct gctcgacacc gtcaccgacc tgatccacgc ccgcaagtcc
     3901 tgagcctcat gcaagccttt gcctccacac ctgcacagac cctgagcgag gaactcgccg
     3961 ccgcgcgtgc cctgctgaaa ctgctgaacg acgagcagga actgctcgtg caggcggatg
     4021 tggacggcct gaatcgcgcc ctggaagaaa aaggcagtct ggttgcgaag atgggtgaac
     4081 tggcgatgcg ccgctaccgt catctaaccg acgccggttt cgatggcagc gaagccggca
     4141 tgcagagctg gctgaacagc cgcaagccgg ccggcaccgc gcagcaggac tgggctgcgc
     4201 tgctcgaact cgcccgccag ggcaaggaac tcaaccgcac taacggcatg ctgatcggcc
     4261 agcatatggc gcgcacccag tcggcgctca acgtgctgca gggcgcgccg gaaggcgggc
     4321 agatgtatgg cccgaacggc caggccacta cggcggccgg cgggcgtcgt cttgtggtgg
     4381 gataggagcc tgtctctgcc gtgctcgagc gcggcttctt gaggtccgca gttctccgcc
     4441 tttcaatcga agtcagtgca tcccatgggc tcccgccttc gcgggagcga cagattgaga
     4501 tgcggctatc gcaatcggcg gcatcgcaga acggggtccg cccgcccagc tatgtcgttc
     4561 ccgcgcaggc gggaacccag ctcggaagca tcaacacgct tcggacgcat taggcacgca
     4621 aaccctgact ctagtgcggc acacacggca ttgcaccccc actcaagcgc ccccacccag
     4681 cggcacctcg gtcgccgtct ccggcagatg cgaaagaatc aggacggcca cgcgccggtt
     4741 gcgctgccgg ccgtcggcgg tctcgttgct gtccaccggc cgcgtctcgc cataaccaac
     4801 ggccgtcagc cgcgtctgcg cgacccccgc atcgacgaac aggcgcacca cgctgctggc
     4861 gcgcaccgcc gacaattccc agttcgacgg gaacgccacg ttgctgatcg gcacactgtc
     4921 ggtatgccct tccacctgga tcgcatggtc gtcgtccttc agcaccgcgg ccaccgcctg
     4981 catcgcatga acgctggccg gcgcaagccg cgcatcgccg ggcgcgaaca gtacgctggc
     5041 attgatctcg accgtcacgc cgcgcggcga ctgggtcaca cgcaccttgc cctgctcgac
     5101 cagcggtgcg agcaccctct ggatgtcgcg cgccatgccg gtgaggcgct cggtttcgcg
     5161 ccgcaacgcg gcatcggccg gagctggaac cggccgcggc cgcggcgccg gcacggccac
     5221 cggcaccacc gactcggcag ggatcggccc cgtctgcacg ggaccggcct gggtgcgccc
     5281 gaacgcattg atcagcgaat tcgacaggat gcggtacttg ccttcgttga ccgaggacag
     5341 cgcatacatc acgacaaaga acgcgaacag cagcgtgatg aaatcggcgt acgagaccag
     5401 ccagcgctcg tggttctcgt gctcctcctc gtgccgcctg cgcctgcggg ccatcgcctc
     5461 agcgcccgta ggcggccagc cgctcggtga cgacgcgcgc attgtcgcca ttggcgacgc
     5521 tgcagaacac gtcggtcagc atttcgcgcc gctcgacctc gtcggcgaca agacccttca
     5581 atttgttggc gatcggcagg aacacgaggt tggcgaagcc gactccgtac acggtcgcaa
     5641 caaaggcgac cgcgatgccg ccgcccaacc ggctcgggtc cgacaggttt tccatcacat
     5701 ggatcagccc gagcacggcg ccgaggatgc cgatggtcgg cgcatagccg ccggccgcct
     5761 cccagatctt caccgcctgg cgctcgcgcc gttcataggc ggaaatgtcg gcctcgagga
     5821 tttcgcggat cttgtacgga tcgatgccgt cgacgatcag ccgcagcccc ttggcggtga
     5881 agccgtcctc gatcgcatcg acgtagcgct ccaggctcag caggccttcc cgccgtgcgg
     5941 tgctgctcca ggtcgacacc tggttggaca gtcccggatc gattcccgcg ggccggcgga
     6001 agatccagcc cagcatgcgc atgccgcgca tgaagctgtc cattccgctt tgcagcagca
     6061 ccgcgccgaa ggtgccgatc accacgatcg taaatgccgc cggctggacc agcgacccga
     6121 tatgtccgcc ctcgagcgcc tgtccggcga ggatgccgaa cagcccgagt gcaaggccga
     6181 tcagactagc ccagtccatg cctgctcccg caggcggttg cgcatgcgcg cgatcgcctg
     6241 gctgtgcaat tgggatacgc gcgattccga cacgcccatc actgccccga tttccttcag
     6301 gttcatctcc tgttcgtagt acagacccat caggatcttt tcgcgctccg gcagtgcctc
     6361 gatggccttg atcaccgcct gccggaaacc ggcgttggtc agcgcctgca gcgggtcgct
     6421 cgcctcgtcc atgatgaagc ggtcgaggaa gtggtcgttc tcgtcgcggt catggaagtc
     6481 ttcgtaatag accagctggt gcccgctgcc ttcatcgagc atcgcctggt attcctccag
     6541 cgagaccttc agctcgccgg cgatttccgc ctcgcgcggc gggcggccca tgcgctgctg
     6601 caccttgttc atcgcggttt cgatcttgcg catgttctgg cgcaccgtgc gcggcagcca
     6661 gtcgctgctg cgcagttcgt cgagcatcgc gccgcggatg cgctgcaccg cataggtctc
     6721 gaactgcgcg ccgtgggtct cctcgtaacg gctgaccgcg tccagcaggc cgatcagccc
     6781 ggcctggatc aggtcgtcga cttccacgct gggcggcagc ttggccttca tctggtgcgc
     6841 cagcttcttg accagcggca cgtgcgtctc cagcaggtcg ttcttgtcgg cttttccgct
     6901 tacggtgtac atgggctcgc gagcaagaat ctgcgatcag cccgccgccg ccgcgtcgaa
     6961 cagcacgacc ttgccggcca ggcggcgaaa cgccaccgat gccccggcga gcggaaaggc
     7021 ctcgaccacc gagcggccca gccgcatcgc gcgcttcaga tgttcgtccg gcggcaccga
     7081 tcccatcgag ttcagcctca cggcgagata gcggttcgcc gcctgcgcca tgttctggta
     7141 gaccagctgc gcctccttgt cgctcgcgcc ggtgaccacc agcgagaacg gccggcggcc
     7201 caactgtccg ttcaggcgct tgatgatcgc gtaggcggac ttgatcgagt ccggcgtgtt
     7261 gaccacctgc acgacgatct cgctcgcggc cagcgcgggg atcgggaatg cgtcgttgcg
     7321 atcgagttcg gcgtcgacca gcaccacgtc ggcgcggcgg gcaagcaggc cgaatgcatc
     7381 atcgagttcc tgcgtaccgc acatgggctg cgcgggccgg cgcacggccg ccacgccgaa
     7441 gccttgcggc aacggctgca ccgcttcctc gatgccgcgc tcgccgcgcg cgacctgcac
     7501 caggctggcg gcgcgcaccg catccagcga gggcgaaatc ccctgcgatt cagtgcatgc
     7561 gtcgagcagc agcacatccc ggccggcctg caccagcgag gcgccgagat tgatcagcat
     7621 cgcgcccttc tcctcgcgcg gcgccgccga cagcagcgtc agcacgcgcg gtttcggtcc
     7681 ggacagcatc cggcgcaggc cttcggcctg atcgaattcg aagctagcca agggacacct
     7741 cgcgcaggga cttgtcgtgc atcgcatggg ccgcgttggc gatcaccagc ggcagctcat
     7801 catccttgta taggtacggg gcggtttcgc gcttgagctt gaacgcgcgg tcgaccagat
     7861 agtgctggtt cgccacgtgc aggtcttccg gcacgcgctg gccattcgcc acgtagtaga
     7921 gattgagctt gtggcggatg atcacgtcga gcacgttgcc aacggtggcc gcctcgtcga
     7981 gtttggtcat gatgcagccg gccaggccgt cgccctgata ggaacgcacc acttcgttga
     8041 gcgtctcgcc ggtggacgta gccgacatgc acagcaggcg cttgaccttc gcgccgctgc
     8101 cggacagcat cgcgatctgc tcggccacca tcttgtcgcg ctggcccatg ccgaccgtat
     8161 cgatcagcac catgtgcttg ttcttcagtt cgtcgagcgc aatgcgcagg tcggtctcgt
     8221 ccttgaccga atgcaccatc acgccgagga tcttgccgta gatgcgcagc tgttcgtagc
     8281 cgccgatacg atagccgtcg gtcgtgatca gcgcgagctt gccggcgccg tgccgcatca
     8341 cgcaccgcgc ggcgagcttg gcggtggtgg tggtcttgcc gacgccggtc gggccgacca
     8401 gcgcatagac gccgcccttc tccaggattt cgttttcgtt gccgatcacc gacaggttgc
     8461 gcgcgagtat cgccttcgtc caggccatgc cgtccttctc gccggccggc aggttctgcg
     8521 tcaggtagcg cgcaaggctg gccgagaaac cggccgacag catctcgcgc atcaacgccg
     8581 ccttcatcgg ctcgcgcttc tgctggctgc tccaggcgat ttcggcgagt tgcgattcca
     8641 gcatgccgcg catcgagcgc agttcgctca tcaacgcggc cagttcgtta ccctgctctg
     8701 tcgtccggct ctggcgcggt ccttcccggc tggccgcaag tgcgccagcc agtccggatg
     8761 cctggtcggt ctcttcgatc gcgcgcggcg cgccgaccgg gggcagcggc gccgccggcg
     8821 cttgctcggc aacgcgcctg gcgccgaggg cgccgagcgt ggcttcggac accgccggct
     8881 tctcgatcac cggctcgccc agcgccgaca tgtcttccgg cgcgagcgcg aggatctcga
     8941 tcgtgccgtt gatcgagcgg ttggacagga tcaccgcatc cggcccgagt gcttcgcgga
     9001 cctggcgcca cgcttcgcgc gacgagtgtg ctgaaaattt tctgacgttc atgcttggcc
     9061 tccgaccagg ctggtgactc taatcgtctt ggtatcggga agttcctggt gcgacagcac
     9121 cttgagctgg ggcagcgtgc ggcgcaggaa acgggacagc agcggccgca gcgggccggg
     9181 cacgagcagc accggcggca ggcccatctg ttcctgctgg cgcgccgcct gctcggtctg
     9241 cgcggcaatc gtgtccgcca gtcccggctc gatgccggca ccgtctgcgc cgccggaatg
     9301 gagcgcctgc atcagcaccc gttcaaggcg gctgtccagt gtgatcaccg acagctcggt
     9361 ggccgacggg aaaatttgct gcacgatcgc gcggcccagc gccacgcgca ccagcgcagt
     9421 gagctcgttc ggatcctgca cctgcggcgc gtattccgaa acggtctcga tgatggtgcg
     9481 catgtcgcgg atgtgcaccc cttcggacag caggttctgc aacaccttct gcagcgtcgc
     9541 cagcggaatc accttgggta ccaggtcctc gaccagcttg gccgaggtct tggcgagatg
     9601 atccagcagc tgctgcactt cctggcggcc gagcagttcg gccgcatgcg tggtgatcag
     9661 atgattcagg tgggtcgcca ccacggtgcc ggcatcgacc accgtataac ccatcgcctg
     9721 cgcctgctcg cgcaatgcgc catcgatcca ggtcgccggc agcccgaacg ccggatcggt
     9781 ggtggcattg cccggcaacg cgccgtgcac catgccggga ttgatcgcca ggaactggcc
     9841 gacataggcc tcgccactgc cgacttccac gcccttcaag gtgaagcgat aggcggtcgg
     9901 ccgcagctcc aggttgtcgc ggatgtgcac cgggggcgac aggaagccga cctcctgcgc
     9961 gaacttcttg cgaatgccct tgatccggcg cagcagctcg ccgttctggc ccttgtccac
    10021 cagcggaatc agccggtagc cgacttccag gcccagcgtg tcgaccggga cgatgtcctc
    10081 ccaggtcgct tcctcgcttt ccggcagcgc gctggcggcg gccgccgcgt tcttctgatc
    10141 gtcttccggt ttctcggcct cctgccgcgc gcgctgcgcc agcaggtagc cgcagccggc
    10201 caacgtcgcg cccagcagca ggaacggcag gttcggcatg ccgggaatca tgcccatgcc
    10261 gccgatgatt gcggcagtga tgtacatcac ctgcggacgc gcgaacagct gcccgaccag
    10321 ctgtcctccg atgtcgtgct cgctggccac gcgcgagacc accacgccgg ccgcggtcga
    10381 gatgatcagc gacggaatct gcgcgaccag gccgtcgccg atggccagga gcgtgtagtt
    10441 gcgcatcgcc gcgtcgaagc ccaggtcgtg ctgggccatg ccgacgatca ggccgccgaa
    10501 gatgttgatc acggtaacga ggatgccggc gatcgcgtcg ccgcgcacgt acttggacgc
    10561 gccgtccatc gcaccgtaga actcggcttc ctgcgcgact tccttgcggc gcgcgcgcgc
    10621 ttcctgctcg ccgatcatgc cggcgttcag atcggcgtcg atcgccatct gcttgccggg
    10681 catcgcgtcg agcgcgaagc gcgcgccgac ttcggcgatg cggcccgcac ccttggtcac
    10741 caccacgaag ttgatgatgg tgaggatgat gaacacgaca ataccgaccg tgtaattgcc
    10801 gccgatcagg aagtggccga atgcctcgat cactttgcca gccgcatccg ggcctgtgtg
    10861 gccttcggtc agcacgacgc gggtcgaggc gacgttgagc gacaggcgca gcatcgtcga
    10921 caccagcagc acggtcggaa acaccatgaa atccagcggg cgcaccgtgt acagcgaggt
    10981 cagcagcacg atgatggcca gcgcgatatt gaagctgaac aggatgtcga gcgcgaacgg
    11041 cggcagcggc agcaccatca tcgccaggat catcacgatc aggatcggcg cggccatcgc
    11101 ccggctgttc gcgccgctca ggaaggcagg caggcgcagg ctgttcattg cgcgcccttc
    11161 acggccgcgt tatgcggatc aagctcgcgc ggcacgtcga ggtcctgcgg ttcctgcggc
    11221 ggcacgccgc catgctgcgc ataggcgcgc agctggaaca cgtaggcgag cacctcggcc
    11281 actgccgtgt acagcgcgct cggaatctcg tcgccgagtt cggcatggcg atacagcgcg
    11341 cgcgccagcg gcggggcttc cagcagcggc accttgtgct cgcgcgccag ttcgcggatc
    11401 ttcgccgcca cctcgtcggc acccttggcc accaccttgg gcgccttcat cttgccctcg
    11461 gtgtacttga gcgcgaccgc ataatggctc gggttggtca ccaccacgtc ggcggtcgga
    11521 acctccgaca tcatgcgccg gcgcgccatt tcgcgctggg tctggcggat acgtgccttg
    11581 acctgcggat cgccgtcggt ctccttcgac tccttgcgca cttcctcgcg cgtcatcttc
    11641 agcttgttcg cgtaatggaa catctgataa ggcgcgtcga tcgccgcgat gatcaccagc
    11701 gccgcgacga tcgcgatgaa gccggtcaaa agcagcgtgc ccaggtgcgc gccggccgac
    11761 tgcagcggct cgaccgccag cgccagcatc gactcgatct gcgtggagat cacaagccag
    11821 gcgatgacac cgaccagaac cgccttgccg attgccttca ccagctccac cagtgcgcgc
    11881 atggagaaca tgttgccgat gcccttgatg gggttcatgc gcgagaactt cggctgcagc
    11941 gccttgccgg agaacagcca gccgccgatc agcagcggcg aagcaagggc cacgagaatc
    12001 aggatggcgc caatcggcgc aaggccgatc gccacatcga acagaccgga ggagagatgc
    12061 tggagcagga gattgaaatc gaacgcatgg gcgcgctcga acgaaagccc ggaggcgagc
    12121 atgccgttca ggcgccgcag cagaccctcg ccggcgagcc agaacgcgat cccgccgccg
    12181 agcaggatcg tgcaggtggc cagttcctta gaccggggaa cgtcgccttc ctcgcgcgcc
    12241 tgctccagtt tcctgggtga cgctggttct gtcttctcga gatcgctgtc ttcggccatc
    12301 gccgactcct gttgggcccc gtgccgccgc gcaaggcagc ggctgaccca tggggggcca
    12361 tagtcaggat tattggcgag ttgtcagttt gacgatcgtc ggaataggcg ttgaaacccc
    12421 gcgttattca gcatcagagc tgcagggtgc gggtccaggc gagtgccgac agctgcgccc
    12481 ggttgacgtc gctcgcttcc acgcacagcg aggtggccat gccatcgagg tcggattcat
    12541 ccgactcgca ggcctcggcc atcgcgagga aggggccgta gatgccctcg cgggtgagca
    12601 gtgcctgggt gatcggctcg gacagctgga tcttctccag caccatctcc atcggcacgc
    12661 cgagcagttt gtcgagcagc gagaacatgc cggcgacgaa caggttttcc gcctcctgct
    12721 tcggcaggaa gcccgctccc agcagttccg cgaagcggcc gcggatcacg gcagtctgca
    12781 tcagcaccgg tgagtagccg gaggtcgtcg cggtcgccag caacaggccg agccagcggt
    12841 acagcggtgc gtagccgagc atcgtcaccg catgcttcag ggactggacc tcgcatccga
    12901 gcccgaatcc ggccgaattg atgtagcgca gcagcttgta ggacaacgcc gcgtcgcgct
    12961 tgagcacact ttcgagctgc ttgacgtccg cgtttttctg caccatctcc atcagctgga
    13021 gaatcagggt ctgagtcgga ttcaggtcgc gtgcgcgcgc gccggggcgc ggcgtcaggt
    13081 acaggccgcc cgcgaagctg aacacgttct gcgcggcaca ggcgtcgaag cgctcccagt
    13141 ccgtcacgcc gcgcgcgacc ggctgcaagg cggcggggcc gagcgcgcgc cactgctgca
    13201 gctgcgcgag gaaatccggg cgatcggcgc gtagctccgc gtacgagagc tgagccagca
    13261 attgcttgtc cagcgcggcc ggattcgcgt cgcgcacgct gaacgcatat ccctgtgcac
    13321 gggccttggc caccgccgca atggtctctt cgctcgccag cgcgtcgccg cgcaagatca
    13381 ggaccgtccc ttgcgcgggc agcaatgcga ggaccgaagg ctcaagcagc gccggggtca
    13441 cttccacgaa cacgcgctgt tcgccgagca aggacgccgt ctcgtcgtca cgcaggtgtt
    13501 catgtacgca gcgcaccagt tccagtgcgg cgttatcgtc cggcgccgtg tcggccgcgc
    13561 catgccagcg gagttcgtaa ccgagcacgc gctcctggtt gttcagcagg ggctcaaggg
    13621 caatgaaatg cgagtcaggc atcttgaagg actgggagtg agaaatggcg ccgaacaggg
    13681 cggcgttcaa aaaccaaggc tgtccagcag gtcgtcgacc tggctctggc tggaaacgac
    13741 atcggcattg ccggtcggat tgatctgcgg gccgttcagc aggccgttcc cggcttcgcg
    13801 gcgcacctct ggcggcgcat agtcgatcag caattgcagc agctgctgct cgatgccgtg
    13861 cgccagttcg gtcacgcgcc ggatcacctg cccggtcaag tcctggaagt cctgcgccat
    13921 catgatgtcc agcagctgct ggcgggtggc gagtgcatcg gcgcgggcac catcgaggaa
    13981 ggccgcggtg cgc
//
LOCUS       DATLGF010000260         6085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_58102,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000260 DATLGF010000000
VERSION     DATLGF010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58102"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..219)
                     /locus_tag="VM406_07845"
     CDS             complement(16..219)
                     /locus_tag="VM406_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75914.1"
                     /translation="MSQPHTKPNRTRNDSANRREINNKDSGDQHLLPEFDTEEAPHRP
                     HRLNREDMDKSPSEDNKGNSSTH"
     gene            399..1298
                     /locus_tag="VM406_07850"
     CDS             399..1298
                     /locus_tag="VM406_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVL75915.1"
                     /translation="MSHPLTAPTALLLTIPPLLWAGNAVVGRIVSTMVPPITLNFLRW
                     AIALVILLPLARAIFRPGSGLLTNWRRYAVLGLLGVGTYNGMQYLALHTSTPINVTLV
                     GASMPVWMLAIGWLFFEARVTRRQMLGALMSIAGVLFVLSRGDWRELLALRLVAGDLF
                     MLVATIIWSVYSWLLTRGHDEPTIRANWATFLAAQVSFGVVWSGAFAGVEWALTDAHI
                     VWSGTLVAALLYVAIGPSLLAFHAWGTGVQRVGPNIAAFFNNLTPLFAALLSSAFLGE
                     PPRLHHAIAFALIAGGIVVSSRR"
     gene            complement(1303..2055)
                     /locus_tag="VM406_07855"
     CDS             complement(1303..2055)
                     /locus_tag="VM406_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL75916.1"
                     /translation="MDRILDNKVAVVTGGGSGIGRAVSLLYAAAGAKVVVSDINEDHG
                     KETVRQCEDAGGTASFLLADTSRAKDNEALVQAAQDRYGALHIACNNAGIGGAAALVG
                     DTPLEEWERVIGVNLSGVFYGMRYQIPAMLAAGGGAIVNMGSILSQVAMREHAPYVAA
                     KHGLMGLTRTAALEYAAKNIRINTVGPAFIRTPLLDNLPAEVFDALTGLHPIGRLGEA
                     NEVAELVLWLSSDKASFVNGGYYAVDGGYLSQ"
     gene            complement(2144..2944)
                     /locus_tag="VM406_07860"
     CDS             complement(2144..2944)
                     /locus_tag="VM406_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HVL75917.1"
                     /translation="MPAHPFDEAIALTRIDDDCFAGHTSEAYWNSISPYGGITVAAVL
                     NALLLHPRRLGDPLAMTINFAGAIKKGPFSIRVHPARTGRSTQHWLIELRQDGDAEPL
                     ITGTAVFATARETWSDIEAAMPPVPQAADLAAAPALRTIPFLERYAMRYVDCNPFAGG
                     ESSLTQCWLSDVPLRKIDFPSLAAFCDSFIPRLFVRKGAPRPVATVTLGINFHVDGPA
                     LEAEQGMHVFTRARANAFNRGYYDQEALVWSESGKLLATTQQMVWYRE"
     gene            3089..3403
                     /locus_tag="VM406_07865"
     CDS             3089..3403
                     /locus_tag="VM406_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75918.1"
                     /translation="MSRPLPAFALLLLLCLAPPAQADCGFAMGAEGMTIVVGKNASAC
                     LAGAGFRAAFATEIRAATVVQPGNGRRLPDDGKARPVRLSALGDMREPQGEPGGRYYG
                     QR"
     gene            3551..4894
                     /locus_tag="VM406_07870"
     CDS             3551..4894
                     /locus_tag="VM406_07870"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HVL75919.1"
                     /translation="MFLLIVSAPLAAVPIRWGEVVEVDTGAGVRGPWQQNRSHYNYID
                     DGSAALDAKGEAAVVWVDQARKQVLFQRYRLEGDGTAGLARPTVVSDSPEVFSWMPRV
                     ALAPDVPGRIYVLWQDIIFSGGSHGGDIMFARSDDGGATFSEPLNLSDSSTGAGKGRI
                     TRDHWHNGSFDLVAAPGGAVHVAWTEFEGRLLYARSLDGGASFAQPLRVAGSDSAPAR
                     GPTLAAGPEGRIYLAWTVGEARGADIHVARSLDGGASFEPARPAARTGAYSDAPKLAL
                     DRRGVLHLAFAESAAGPFDRFRVHYARSTDGGLSFETPREISTPVPEGAISAHFPHLA
                     VDGAGNPVVAWELHPQRVARPRGMGIAVSLDGGETFSAPAAVPHSADPGGGPNGSQQG
                     LLMRKLAVGPDGRLVIAQSALRENEFSRVWLIHGRLDRRAGGTETGAAAFATTGE"
     gene            complement(4951..5172)
                     /locus_tag="VM406_07875"
     CDS             complement(4951..5172)
                     /locus_tag="VM406_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75920.1"
                     /translation="MRVKAIIEDEHERSSVEFSLEEMIGHSPGLGWSEMDEEGRLHAL
                     EDYAAWLYTRQSGTGVRSRVRIDPSTLPR"
     gene            complement(5365..5790)
                     /locus_tag="VM406_07880"
     CDS             complement(5365..5790)
                     /locus_tag="VM406_07880"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HVL75921.1"
                     /translation="MKRTASASWSGGLKDGKGKLSTHSGALDNLPYDFADRFESGPGT
                     NPEELIGAAHAGCFTMFLAGKLAEAGMKAEQLQTTATVSLDKVDGGFSITAVHLDLTA
                     RVTGGDRAAFENAVKLSKEGCPVSKVLNAAITVDMRLES"
     gene            5923..>6085
                     /locus_tag="VM406_07885"
     CDS             5923..>6085
                     /locus_tag="VM406_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HVL75922.1"
                     /translation="METLNWEEGGKTQTARWRSEAGVPPHKRVIVADDRIAADAAYKL
                     ACEGTALLWR"
BASE COUNT          921 a         1997 c         2050 g         1117 t
ORIGIN      
        1 acgcttagcc cgcgctcagt gcgtggagct gttgcccttg ttgtcttccg acgggctctt
       61 gtccatgtcc tcgcggttca ggcggtgcgg gcgatgcggc gcttcttccg tatcgaattc
      121 cggcagcagg tgctgatcgc ccgaatcctt gttgttgatt tcgcggcggt tcgcggaatc
      181 attgcgggtg cggttcggct tggtgtgcgg ttgcgacata cgatcttcct ttgctgattg
      241 cgtccgagtg cggttagagg ggagccgccc gtccgcggtt cagcaggcgt gccaccgcga
      301 tggcgcgcat cgtctttgcg cgggttcgca gtctcggctg caacatctcc cgcgaatccg
      361 ttatcctcgc aagcttcccc gctccaaccc ctgtcttcat gagccatccg ctgaccgccc
      421 cgaccgcact cctgctcacg atcccgccgc tcttgtgggc gggcaatgcg gtggtcggtc
      481 gcattgtcag cacgatggtg ccgccgatca cgctcaattt cctgcgctgg gcgatcgcgc
      541 tcgtgatcct gctgccgctg gcacgcgcga tcttccgacc cggcagcggc ctgctgacca
      601 actggcgccg ctatgcggtc ctggggctgc tgggcgtggg cacctacaac ggcatgcaat
      661 acctcgcgct gcacacgtcc acgccgatca acgtcacgct ggtgggcgcg tcgatgccgg
      721 tgtggatgct ggcgatcggc tggctgttct tcgaggcgcg cgtcacgcgt cgccagatgc
      781 tgggcgcgct gatgtcgatt gccggcgtgc tgttcgtgct ctcgcgcggc gactggcgcg
      841 aactgctggc gttgcggctg gtggccggcg atctgttcat gctggtggcg accatcatct
      901 ggtccgtgta cagctggctg ctgacgcgcg ggcacgacga gccaacgata cgcgcgaact
      961 gggccacctt cctggccgcc caggtcagct tcggcgtggt gtggtcgggc gcgtttgccg
     1021 gcgtcgaatg ggcgctgacc gatgcgcaca tcgtctggag cggtacgctg gtggcggccc
     1081 tgctgtatgt ggcgatcggc ccgtcgctgc tcgccttcca cgcctgggga accggcgtgc
     1141 agcgggtcgg gccgaacatc gcggcgttct tcaacaatct gacgccgctg ttcgcggcgc
     1201 tgctgtcgag cgcctttctc ggcgagccgc cgcgtctgca tcacgcgatc gcgttcgcgc
     1261 tgatcgcggg cgggatcgtc gtgtcgtcgc ggcgctgaac gctcactgcg acaggtagcc
     1321 gccgtccaca gcgtagtagc cgccgttgac gaaggatgcc ttgtccgagc tcagccacag
     1381 caccagctcg gccacttcgt tggcttcacc gagccggccg atcggatgca gcccggtgag
     1441 cgcatcgaac acttcggccg gcagattgtc cagcaacggc gtcctgatga aggccggccc
     1501 gacagtgttg atgcgaatgt tcttcgccgc gtattcgagc gccgcggtac gggtcaggcc
     1561 catcaggccg tgcttggccg ccacgtaggg cgcatgttcg cgcatcgcga cctgcgacag
     1621 gatggaaccc atgttgacga tcgcgccgcc gcccgcggcc agcatggccg ggatctggta
     1681 gcgcatgccg tagaacacgc ccgagagatt cacgccgatc acgcgttccc attcttcgag
     1741 cggggtgtcg ccgaccaggg ccgcggcgcc gccgatgccc gcattgttgc aggcgatgtg
     1801 cagcgcgccg tacctgtcct gcgcagcctg caccagtgct tcattgtcct tggcacgcga
     1861 ggtgtcggcc aaaaggaagc tggccgttcc gccggcgtct tcgcattgcc tgacggtttc
     1921 cttgccgtgg tcctcgttga tgtcagagac caccaccttc gctccggccg ccgcgtacag
     1981 cagcgagacc gcccgcccga tgccggatcc gccgccggtt acgaccgcca ccttgttgtc
     2041 gagaattctg tccatgtcgg ctctccttga aattgtccga cgatggtagg aaggcaggtc
     2101 acgcacgccc ttgatccagg tcaatttcgc ccggggagta ccctcattcg cgataccaga
     2161 ccatttgctg tgtcgtggcc agcagcttgc cggattccga ccagaccagc gcttcctgat
     2221 cgtagtagcc gcggttgaac gcgttggccc gcgcgcgggt gaatacgtgc atgccttgtt
     2281 cggcttccaa cgctgggccg tcaacatgga agttgatgcc gagcgtaacg gtcgcgacag
     2341 gacggggtgc gcccttgcgc acgaacaggc gcgggatgaa ggaatcgcag aacgcggcca
     2401 gcgaagggaa gtcgatcttg cgaagcggca cgtcggagag ccagcactgg gtgaggctgc
     2461 tctcgccgcc ggcgaacgga ttgcagtcga catagcgcat cgcgtagcgc tcgaggaagg
     2521 ggatcgtgcg cagcgcgggt gcagcggcga ggtcggccgc ttgcggtacg ggcggcatcg
     2581 ccgcctcgat atcggaccag gtctcgcgcg ccgtggcgaa tacggccgtg ccggtgatca
     2641 gcggttcggc atcgccgtcc tggcgcagct cgatcagcca gtgctgcgtc gagcggccgg
     2701 tgcgcgcggg gtgcacacgg atggaaaacg gccccttctt gatcgcgccg gcgaagttga
     2761 tggtcatggc cagcgggtcg ccgagccggc gcgggtgcag cagcagcgca ttgagcacgg
     2821 cggcgaccgt gatgccaccg tacggcgaga tcgagttcca gtaggcttcg ctggtgtggc
     2881 cggcgaagca gtcgtcgtcg atgcgcgtga gcgcgatcgc ttcatcgaaa gggtgggcag
     2941 gcatggcggc ttggcggatc aggttaacga acggtcgttc ggataatgat acgcaagtcg
     3001 cgggatgcct gcttttgccc cagccagtct gcggtgcacg cgggagtgga ataaggtata
     3061 tttccaagcg gaaataaagt tcactcccat gtcacgtccg cttcccgcgt ttgccctcct
     3121 cttgcttctg tgcctcgcgc ccccggcgca ggccgactgc ggcttcgcaa tgggcgccga
     3181 gggcatgacc atcgtcgtcg gcaagaacgc cagtgcctgc cttgccgggg ccggattccg
     3241 tgctgccttt gcgaccgaga tccgcgccgc cacggtcgtg cagccgggca atggccgccg
     3301 cttgccggac gatggaaagg cgcgcccggt gcggctgtcc gcgttggggg acatgcgcga
     3361 gccgcagggc gagcccgggg ggcgttacta cggccagcgt tagggcgcta ggttccgaga
     3421 ccgctgcggc gcttgaggaa tatcatccgc cggtccgcat cccccgcgtt cttgccaacc
     3481 tggtctttgc aagcccggcc ttctgagtaa gatgcagcgc atgcggccgc gcctgtatcg
     3541 attactttgc atgttcttgc tgatcgtcag cgcgccgctg gcggccgtgc cgatccgctg
     3601 gggcgaggtg gtcgaagtgg ataccggcgc gggcgtgcgc ggtccctggc agcagaaccg
     3661 ctcccactac aactacatcg acgacggcag cgcggcgctg gacgcgaaag gcgaagcagc
     3721 agtcgtttgg gtggaccagg cgcgcaagca ggtgctgttc cagcgctatc ggctcgaagg
     3781 ggacggcacc gccggccttg cgcggccgac cgtggtgtcg gacagccccg aggtcttttc
     3841 atggatgccg cgcgtcgcgc tggcgccgga cgtccccggc cgcatctatg tgctatggca
     3901 ggacatcatc ttctccggcg gctcgcacgg cggcgacatc atgtttgccc gctccgacga
     3961 cggcggcgcg accttctccg aaccgctcaa cctgtcggac tccagcaccg gcgccggcaa
     4021 gggccgcatc acccgggacc actggcacaa cggcagcttc gacctcgtcg ccgcgcccgg
     4081 cggcgcggtg catgtcgcgt ggaccgaatt tgaaggaagg ttgctctatg cgcgttcact
     4141 cgacggcggc gcaagctttg cgcagccgct gcgcgtggcc ggcagcgaca gcgcgccggc
     4201 acgtggcccg acgctggcag ccggccccga aggccgcatc tacctggcct ggaccgtagg
     4261 cgaagcgcgc ggcgccgaca tccatgtggc gcgctcgctg gatggcggcg cgagcttcga
     4321 gcccgcgcgg ccggccgcgc gcaccggcgc ctattccgat gcgccgaagc tggcgctgga
     4381 ccggcgcggc gtgctgcatc tggccttcgc cgaaagcgcg gccggaccgt tcgaccgttt
     4441 ccgcgtgcac tatgcgcgct cgaccgacgg cgggctttcc ttcgagacgc cgcgcgaaat
     4501 ctccacgccg gttcccgagg gcgcgatcag cgcccacttt ccgcatctgg ctgtcgatgg
     4561 tgccggcaat ccggtggtgg cgtgggaact gcatccgcaa cgggtcgcgc gcccgcgcgg
     4621 catgggcatc gccgtctcgc tcgatggcgg cgaaacgttt tccgcgccgg cggcagtgcc
     4681 gcacagcgcc gatccgggcg gcgggccgaa cggcagccag cagggcctcc tgatgcgcaa
     4741 actggccgtc gggcccgatg gcaggctggt gatcgcccag agcgcgctgc gcgaaaatga
     4801 attcagccgg gtctggctga tccacggccg cctggatcgg cgcgccggcg gcaccgagac
     4861 cggcgcagct gctttcgcga caaccggaga ataactgcgg tgcgtggtgc ttgcggtgcg
     4921 gccggcgagg cgggcacccc ttagggccaa tcaacgcgga agtgtcgacg ggtcgatgcg
     4981 cacgcgtgaa cgcacgccgg tgccgctctg gcgggtgtac agccacgcgg catagtcttc
     5041 gagcgcatgc aggcgtcctt cctcgtccat ctcgctccag cccaaaccgg ggctgtgccc
     5101 gatcatttcc tcgagcgaaa actcgacgct gctgcgctcg tgttcatctt cgatgatcgc
     5161 ttttactctc attgcgagtc ctccttcaaa cccgcattga aagcgaaacg cgtgcctgca
     5221 gccgctgaaa gagcgccaag tccttgtcgg agaagcggca gaacaccacg tgctcaatca
     5281 ccgtccacac ccggcagcat gctctccgcc aggatggccg tgatgcgtgc aggcatgaca
     5341 ttcctttgaa gaatttgcac ccgctcagct ctctagccgc atgtccaccg tgatcgccgc
     5401 attgagcact ttcgagaccg gacagccttc cttggacagt ttcacggcat tctcgaacgc
     5461 ggcgcggtcg ccgccggtca cgcgggcggt gaggtcgaga tgcaccgcgg tgatcgaaaa
     5521 gccgccgtcc accttgtcca gcgacacggt cgccgtggtc tgcaattgtt cggccttcat
     5581 gccggcctcg gccagctttc cggccaaaaa catcgtgaag cagccggcgt gagccgcgcc
     5641 gatcagttct tccggattgg tgccggggcc gctttcgaaa cggtcggcga aatcgtacgg
     5701 caggttgtcg agtgcgccgc tgtgcgtgga aagcttgccc ttgccatcct tcaggccgcc
     5761 gctccacgag gcgcttgcag tgcgtttcat cgcattctcc ttgcgggtga gtcaagcaag
     5821 cattgcagca gcaaggcgcg cgaacagctt gagcggtctc aatccggggc gtgtaccgcg
     5881 cgaggctggc cccgagacgc ctctgcgcgt aagcttctgc cgatggaaac attgaactgg
     5941 gaagaaggcg gaaagaccca aaccgcgcgc tggcgctcgg aagccggggt gccgccgcac
     6001 aagcgcgtga tcgtcgccga cgaccgcatc gccgccgatg ccgcctacaa gcttgcctgc
     6061 gaagggacgg cgctgctgtg gcgcg
//
LOCUS       DATLGF010000261         3518 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_58114,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000261 DATLGF010000000
VERSION     DATLGF010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58114"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1264)
                     /locus_tag="VM406_07890"
     CDS             complement(53..1264)
                     /locus_tag="VM406_07890"
                     /inference="COORDINATES: protein motif:HMM:NF042658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system protein"
                     /protein_id="HVL75923.1"
                     /translation="MTASSVSTFDASASMLGYLYQVRIALLWAIRQSRVGDFAVSIET
                     LDDVSFETGGDPMAVLQTKHSLNAAAVLSDLSPDLWKTLRIWMVGLASGEVPASASRF
                     LISTAHAPSGTACSALGIDAVTRNVAEAARRLKHAATTSSNAELQSAFESYLELGDEA
                     REQLLSRIYVVSAQPDALAIQEQLNAELYHVSLHHQELSVQMLEGWWFKRVLQELVYQ
                     GQGILRAEIDAQIADIQESLKPDSLPIDEEIDALMVALDQLPEFASRPFYKQIELVGG
                     SQTRIRNAITSYLQAFRQRSAWTRHNLLFDADLEKYDRRLLTEWELQRAQVCDELGPE
                     ATAAAMASAGRAILKWAEDAHVPIRTGVSVPWVCRGSLHMLAEDRRLGWHPEFETRLK
                     AIFDTPVGDSA"
     gene            complement(1344..2822)
                     /locus_tag="VM406_07895"
     CDS             complement(1344..2822)
                     /locus_tag="VM406_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012366289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HVL75924.1"
                     /translation="MDSAIARSRATVREDGYVHEQYPPAARPEETLAGHLTFALKHEG
                     VHLEFLSRLFETVPAAELEAWVAAEPTGQYSRRAGFFYEYLTGRQLAFPGVVAGNYVA
                     ALNSEAYLTASQPLNNPRWRVRDNLPGSREYCPLVLRTARVQQAELYDCAQHLADLEA
                     EFGADVLQRSAVWLTVKESRASFAIEHEEQHVDRVRRFAAVMEHWCGRHDDPLSEVTL
                     GELQAQILGPRATRYGVRRSPVFVGEVNGFTEVVHYIAPHWDHAPRLLTGLRDCALRT
                     AGGSPLLRAALLSFGFVYIHPMSDGNGRISRFLINDVLRRDGAVPEPFILPVSATITS
                     SVINRRGYDQILELFSRPLMRRYADAWRFGPDQVAEDGVHFNLQFDAYQDALPAWRYP
                     DMTAHVEYLAEVVRLTIDQEMRKEAGYLRNLRLARERVKQVIEGPDGDIDRIIRSVRD
                     NGGKISNKLIKEIPALADEHLAANVAAAIQAVFLPPGEEDRA"
     gene            3254..>3518
                     /locus_tag="VM406_07900"
     CDS             3254..>3518
                     /locus_tag="VM406_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HVL75925.1"
                     /translation="MLSLFKRKRVPSIAGAPPTPTSPAEPGKGLMRPKSAASLLATPR
                     RQKLLEHIWQRTSLSRRQFTTLYLAPLERYAELVQQFPASESHH"
BASE COUNT          695 a         1100 c         1059 g          664 t
ORIGIN      
        1 aacgctggat tcagcaggtt gcgctgctcg attgttcgtt cctgccaagc tgtcatgccg
       61 aatcacctac aggagtatcg aagatggctt ttagccgcgt ctcgaattcg ggatgccacc
      121 ccaaccgacg gtcttccgcg agcatgtgca gcgagcctcg gcagacccac gggacgctga
      181 cacccgtgcg gatcggtacg tgtgcatcct cagcccactt gaggatggcg cgaccggccg
      241 acgccatcgc tgccgccgtc gcctccgggc ccaactcatc gcacacctgg gctcgttgca
      301 gttcccattc cgtgagcagg cgacggtcgt atttctcaag gtcagcatcg aagagcaaat
      361 tgtggcgggt ccatgctgat cgttggcgga aagcctgcag gtagctcgtg atcgcgtttc
      421 gaatccgggt ctggctgccg ccgaccagtt cgatctgctt gtagaacggg cggctggcga
      481 actcgggtag ttgatccaac gccaccatca acgcatcgat ctcctcgtcg atcggcagcg
      541 aatctggctt cagggactcc tgaatgtccg cgatctgtgc atcgatttcg gccctgagaa
      601 tgccttggcc ttgataaacg agttcctgga gcacacgctt gaaccaccag ccctccagca
      661 tctggaccga gagctcttga tggtgcagcg agacatggta gagctcggca ttcaactgct
      721 cctggatagc cagagcatct ggctgggccg acacgacata gatgcgggag agcagttgct
      781 cgcgggcctc atcacccaac tcaagatagg actcgaacgc actctgaagc tcggcgttgg
      841 aggaggtggt ggctgcatgc ttcagccgcc ttgccgcctc ggccacattg cgcgtcaccg
      901 cgtcgatgcc gagcgctgag caagccgttc ccgatggcgc atgggccgtg gaaatgagga
      961 atcgtgaggc agaggcaggc acctcaccac tggcaagccc caccatccag atgcgcaggg
     1021 tcttccagag atcgggactg agatcgctca gcactgccgc agcgtttagc gagtgcttgg
     1081 tctggagaac cgccatcgga tcaccgcccg tctcaaaact cacatcgtcg agggtttcga
     1141 tgcttaccgc aaagtctccg acgcggcttt gtcggatcgc ccacaatagc gcgatgcgca
     1201 cctggtaaag atagccgagc atggaagcgc tggcgtcaaa cgtggatacg ctggatgcgg
     1261 tcacggcgat caccttactt gttgcctggc ggataaggcc acttcatcca ggccaatgga
     1321 gcgaatgtgg aatcgatgaa tcgtcatgcg cggtcttcct cccccggtgg caggaatacc
     1381 gcctggattg ctgcagcgac gttcgcggcg aggtgctcgt ccgcaagcgc cggtatttcc
     1441 ttgatcagct tgttcgaaat ttttccgccg ttgtcccgca cagatcggat gatgcgatcg
     1501 atgtccccgt ccggcccttc aatgacctgc ttgacgcgct cgcgcgcgag ccgaaggtta
     1561 cgcaaatagc ccgcttcctt gcgcatttcc tggtcgatgg tgaggcgcac gacttcggca
     1621 aggtattcga catgcgccgt catatccgga tagcgccaag ctggcaacgc gtcctggtaa
     1681 gcatcgaact ggaggttgaa atgcacgcca tcttccgcga cctgatcagg gccgaaccgc
     1741 caagcgtcgg cgtaccgccg catgagcggt cgcgagaaca gttcgaggat ctggtcgtat
     1801 ccgcgacggt tgatcaccga actggtaatc gtggcggata ccggcaggat gaacggctcg
     1861 ggtacggctc cgtcgcgcct aagcacgtca ttgatcagga agcgtgagat tcgcccgttg
     1921 ccgtcggaca tcggatggat gtagacgaag ccgaacgaca acaaggccgc ccgcaacagc
     1981 ggcgagccgc cggccgttcg cagggcacag tcgcgtaagc cggtcaggag gcgcggcgca
     2041 tggtcccaat gcggcgcgat gtagtgaacg acctcggtga agccgttcac ctcaccgaca
     2101 aacaccggcg agcggcgcac gccgtagcgt gttgccctcg gccccaggat ctgggcctgc
     2161 agttcgccca gcgtcacctc ggacagcgga tcgtcatgcc tgccgcacca gtgctccatc
     2221 actgccgcaa agcgtcgcac gcgatcgacg tgttgctctt cgtgctcgat cgcaaagctg
     2281 gcccggctct ccttgacggt caaccagacc gcgctgcgct gcaggacatc ggcgccaaac
     2341 tccgcctcca ggtccgcgag atgttgggcg cagtcataca gttccgcctg ttgcacccgc
     2401 gccgtgcgca gcaccagcgg acagtattcc cgcgagcctg gcaagttgtc gcgcacacgc
     2461 cagcgcgggt tgttgagggg ctggctggct gtcaggtagg cctcgctgtt gagcgccgcc
     2521 acatagttgc ccgcgaccac gccgggaaag gccaactgcc gtccggtcag gtactcatag
     2581 aagaacccgg cgcgccgcga gtattgaccc gtgggctcgg cggcgaccca tgcctccagt
     2641 tctgcagcag gtaccgtctc aaagagccgc gacaggaatt ccaggtggac gccttcatgc
     2701 ttgagtgcaa aagtcaagtg gccggccagg gtttcctccg gcctagccgc aggcgggtac
     2761 tgttcgtgga cgtaaccgtc ttcgcgcact gttgcgcgcg acctcgcgat ggcactatcc
     2821 acgcgcaaag cttgcacggt agagacgccg tagcgttcag ccaaccatcg atatccgatc
     2881 cagtctggca cttaggatga cggggcgagc ccgcagaata attagatgtt gaagattttg
     2941 attatttttt ggcatgcggt caatattttg attacagccg acccggaagc agctcgaacc
     3001 ttacatcccc ggtgtcaagc ctcgggagac tgacgaattg tcagatcagt ccggcgtgcc
     3061 ggattccccg ccttaatccg acctgggcca aatccacatt ggttgtggct ggactggtgt
     3121 cgcggcccta tacccaaagg cttccataaa agccaaaggg ctgggaaggg ggcaagggaa
     3181 gggggccaat gggaaaggtc ctacccgaaa aggcaaaaag gctccccgag cagcccgaca
     3241 tccggggttt accatgctct cgctgttcaa acgcaaacgg gtgccgtcca ttgccggcgc
     3301 gccaccgacg cccacatctc ccgccgagcc cggcaaaggg ctgatgcggc cgaagtcggc
     3361 ggcatcgctg ctggcgacac cgcgccggca gaagctgctg gaacacatct ggcagcgcac
     3421 ctcgctgtcg cgccggcagt tcaccacgct ctacctcgca ccattggaac gctacgccga
     3481 gctggtccaa cagttcccgg cttcggaaag ccaccacc
//
LOCUS       DATLGF010000262         2878 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_70331,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000262 DATLGF010000000
VERSION     DATLGF010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70331"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="VM406_07905"
     CDS             <1..952
                     /locus_tag="VM406_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HVL75926.1"
                     /translation="RHLHTPSAVSFEIGMGAVAGDPAAIRIDSGIRQGDAISPFYDPM
                     VAKLIVWGNDRAEALAHMSQALSEYQVVGLATNVAFLKRLVESQPFSQADLDTGLIER
                     NHAALFPPAPAIELPTLALAAASLLASEQTAGAAANDPWADTSGWRMNATFKRTLDLS
                     DENDKYPLTVAYEDKGWTVQAGERGAAMTLIERDGQHLVIKLDGATVRGTVVRDGEMF
                     HVFSGGAHIALHYNDPLAHAGEAEGEGGRLTAPMPGKIVALLVEKGKTVEKGAPLLIM
                     EAMKMEHTIAAPANGTVEELLYGVGDQVAEGAQLLAFKVA"
     gene            1035..1952
                     /locus_tag="VM406_07910"
     CDS             1035..1952
                     /locus_tag="VM406_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HVL75927.1"
                     /translation="MTLPTKVKLVEVGPRDGLQNEKETIPAEVKIELVDRLTDAGFSN
                     IEAASFVSPKWVPQMATSAEVMEKITRKPGVIYSALTPNMKGFEAALAAKADEVVIFG
                     AASEAFSQKNINCSIAESIERFREVAEAAKRNNIRLRGSISCTMGCPYQGEVPLDAVA
                     DVVRRMRDLGCDEIDIADTIGVGTPRKVQAVMERAAREIPIAQLSGHFHDTYGQALAN
                     IYASMEVGVSIFHSSVAGLGGCPYAKGATGNVSTEDVLYMMNGLGIETGIDLDKVVDA
                     GQFISRHLGRKAASRAGNAIWAKREGASC"
     gene            1955..2437
                     /locus_tag="VM406_07915"
     CDS             1955..2437
                     /locus_tag="VM406_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015878075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL75928.1"
                     /translation="MRIREITPSEFGLVWPIFHAVVASGDTYAYDPSTSFEEAKAMWT
                     TPPARVFVAEEGDLVLGCYVLKPNQGGLGDHVANAGYMVAPDARGKGVASALCEHSLD
                     AARAAGFSAMQFNFVVASNTTAVALWLKHGFEIVGRVPNAFRHRTQGLTDVFVMYRPL
                     "
     gene            2442..>2878
                     /locus_tag="VM406_07920"
     CDS             2442..>2878
                     /locus_tag="VM406_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HVL75929.1"
                     /translation="MDEPLQDLLARARACRLCEVHLPHGPRPVLRASTSARLLIVGQA
                     PGRRVHASGIPWDDASGARLRDWMGVSPEVFYDERRVAILPMGLCYPGTGRSGDLPPR
                     PECAAAWHAPLLAQLQKTELILLVGRYAQAHYLGENGRPTLSET"
BASE COUNT          521 a          931 c          976 g          450 t
ORIGIN      
        1 gcgtcacctg cacacgccgt cggcggtgtc gttcgagatt ggcatgggcg cggtagccgg
       61 cgatccggcg gcgatccgga tcgattccgg catccgccag ggcgatgcga tctccccgtt
      121 ctacgacccg atggtcgcca agctgattgt ctggggcaat gaccgcgccg aggcgctcgc
      181 gcacatgtcg caggccttgt ccgaatacca ggtcgtcggc ctggcaacca atgtcgcctt
      241 cctgaagcgc ctggtggaaa gccagccgtt ctcgcaggcc gacctcgata ccggcctgat
      301 cgagcgtaac catgccgcgc tgttcccacc ggcaccggcc atcgagttgc cgacgctggc
      361 gctggcggcc gcctcgctgc tggcatcgga acagacggcc ggcgcagcgg cgaatgatcc
      421 ctgggccgat accagcggct ggcgcatgaa tgcgaccttc aagcgcacgc tcgacctgtc
      481 cgacgagaac gacaagtatc cgttgacggt tgcctatgaa gacaagggct ggaccgtgca
      541 ggcgggcgag cgcggcgccg cgatgacgct gatcgagcgt gacggccagc acctggtgat
      601 caagctcgac ggcgcgacgg tgcgcggcac cgtggtgcgc gacggcgaga tgttccacgt
      661 cttttccggc ggcgcgcaca tcgccctgca ttacaacgac ccgcttgcgc atgccggcga
      721 agccgaaggc gaaggtggcc gcctgaccgc gccgatgccg ggcaagatcg tcgcgctgct
      781 cgtggagaag ggcaagacgg tggagaaggg cgcgccgctg ctgatcatgg aagcgatgaa
      841 gatggagcac acgatcgccg caccggccaa tggcacggtc gaggaactgc tgtacggggt
      901 cggcgaccag gtggcagaag gcgcgcagtt gttggcgttc aaggtggcgt aggaaaagcc
      961 ccgaagaaca agtcccctcc ccctgaaagg gggaggaagt cttacggtgg cacggaacaa
     1021 tcaaggtgaa ccccatgacc ctcccaacca aagtcaaact cgtcgaagtc ggcccgcgcg
     1081 acggcctgca gaacgagaag gaaaccatcc cggccgaggt gaagatcgaa ctggtcgacc
     1141 gcctgaccga tgccggcttc tccaacatcg aggccgcgtc cttcgtgtcg ccgaagtggg
     1201 tgccgcagat ggcaacctcg gccgaagtga tggagaagat cacgcgcaag cccggagtca
     1261 tctattcggc actgacgcca aacatgaaag gcttcgaagc cgcactcgcc gcgaaggcag
     1321 atgaagtggt gatcttcggc gcggcttccg aggcgttttc acaaaagaac atcaactgct
     1381 cgatcgccga gtcgatcgag cgcttccgtg aagtggccga agccgccaag cgcaacaaca
     1441 tccgcctgcg cggcagcatc agctgcacga tgggctgccc gtatcagggc gaggtgccgc
     1501 tggacgcggt ggcggacgtg gtgcggcgca tgcgcgatct cggctgcgac gagatcgaca
     1561 tcgccgacac catcggcgtg ggtaccccgc gcaaggtgca ggcggtgatg gagcgcgcgg
     1621 cgcgcgaaat tccgatcgcg cagttgtccg gccacttcca cgacacctac ggccaggcac
     1681 tggcgaacat ctacgcgagc atggaagtcg gcgtatcgat cttccattcc tcggtcgccg
     1741 gcctgggcgg ctgcccgtat gcgaagggtg cgaccggcaa tgtctcgacc gaagacgtgc
     1801 tgtacatgat gaacggcctc ggcatcgaga ccggcatcga tctggacaag gtggtcgacg
     1861 ccggccagtt catttcgcgg cacctcggtc gcaaggcggc cagccgcgcg ggcaacgcga
     1921 tctgggccaa gcgcgagggt gcgagttgct gaggatgcgg atacgcgaaa tcacgccgtc
     1981 cgagttcggc ctggtctggc cgatctttca cgcggtggtc gcaagcggcg acacctacgc
     2041 ctacgatccg tcgacgagct ttgaagaggc caaggcgatg tggaccaccc cgccggcgcg
     2101 cgtgttcgtt gccgaggagg gcgatctggt gctcggctgc tacgtgctca agccgaacca
     2161 gggcggactg ggcgaccatg tcgccaacgc cggttacatg gtcgcgccgg atgcgcgcgg
     2221 caagggcgtg gccagcgcgc tgtgcgaaca ctcgctggac gccgcgcgcg ccgccggctt
     2281 ttccgcgatg cagttcaact tcgtggtcgc ctccaacacc accgcggtcg cgctctggct
     2341 caagcacggc ttcgagatcg tcggccgcgt gccgaacgca ttccgccacc gcacgcaagg
     2401 cctcaccgac gtcttcgtga tgtaccggcc gctttaacgc gatggatgaa ccgctgcagg
     2461 acctgcttgc ccgtgcccgc gcctgccgcc tgtgcgaggt ccacctgccg cacggtccgc
     2521 gcccggtgct gcgggcgagc acgagcgcaa ggctgctgat cgtcggccag gcgccgggtc
     2581 gtcgtgtgca tgcgagcggc attccctggg acgatgcatc cggcgcgcgc ctgcgcgact
     2641 ggatgggcgt gtcgcccgag gtgttctacg acgagcgcag ggttgccatc ctgcccatgg
     2701 gcttgtgcta tccgggaacc ggcaggagcg gcgatctgcc gccgcgaccc gaatgcgccg
     2761 ccgcctggca cgcgccgctg ctcgcgcagc tgcaaaaaac cgaactgatc ctgctggtgg
     2821 gacgctatgc gcaggcccac tacctgggcg agaatggcag gccgacgctg tccgagac
//
LOCUS       DATLGF010000263        16033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_70354,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000263 DATLGF010000000
VERSION     DATLGF010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 16033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16033
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70354"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..736)
                     /locus_tag="VM406_07925"
     CDS             complement(167..736)
                     /locus_tag="VM406_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HVL75930.1"
                     /translation="MEAFLLSTGIVALAEMGDKTQLLSLLLAARFRRPLPIILGIFVA
                     TLLNHALAGALGALLSDWLGPDVLRWILAISFLAMAVWVMVPDKLDDVDVKPAAAGVF
                     GVTTAAFFLAEMGDKTQIATVALAAKFDAFGAVVIGTTLGMMIANVPAVLLGDRVTRM
                     VSMKVVHGVAAVLFALLGVATLFNVGGMF"
     regulatory      complement(739..929)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
     gene            complement(974..1915)
                     /locus_tag="VM406_07930"
     CDS             complement(974..1915)
                     /locus_tag="VM406_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HVL75931.1"
                     /translation="MNLHQLRFVREAVRQNFNLTEAAKALYTSQPGVSKAIIELEEEL
                     GVDIFTRHGKRIRGLTEPGRAVLRSVELIMQEIEGLKQIGKEFAAQDTGSFTIATTHT
                     QARYALPKVVQAFTQKYPKVRLSLLQGNPKQVTEMVQRDQADLAIATEFIADAEGLIS
                     LPCYQWEHVVVVPPDHPLQKANPITLEDIARYPLITYDSAFTGRAKIDHAFQIRHLKP
                     DVLLEAIDADVIKTYVELGMGVGIIAGMAFDPERDKGLRAISVGHLFGTNVSRVALKQ
                     GAYLRSYVYSFIELLSPTLTRKLVEQVMSGEKDSYEL"
     gene            2104..3798
                     /locus_tag="VM406_07935"
     CDS             2104..3798
                     /locus_tag="VM406_07935"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HVL75932.1"
                     /translation="MYRYDEYDHQIIRERVAQYRDQVRRRLSGELTEDEFRILRLQNG
                     LYYQRHAYMLRVAIPYGLLSSKQVRMLAHIARDYDRGYGHFTTRQNIQYNWMELEQTP
                     EILEKLASVEMHAIQTSGNCIRNITSDPYAGIAPDEIVDPRPYCEILRQWSTFHPEFA
                     YLPRKFKIAINGAAEDRAAIMVHDIGLTVLKNEAGEVGFRVFVGGGMGRTPILGSEIR
                     EFLPWQHVLSYIEAIVRVYNQYGRRDNIYKARIKILVKAIGIEEFRRQVEEEWQQIKD
                     GPSTLTQEELDRVAAHFTAPAYEALPADDAKLDAFQAENKAFANWVKRNVKAHRVPGY
                     AAVVLSLKKTGVPPGDATDEQLDFAADIAERYSFGELRVTHEQNLVLSDVKKSDLIAL
                     WEEIKAQGLATPNIGLLTDMICCPGGDFCALANAKSIPVAGEIAERFDNLDFQHDIGE
                     IELNISGCINSCGHHHVGHIGILGVDKDGSEWYQISVGGAQGNASSIGKIIGPSFSAP
                     QVPEVIDRLLKVYVRERHEDERFIDTARRIGIAPFKEYVYATPIKTEQNAAEDAHV"
     gene            3791..4312
                     /locus_tag="VM406_07940"
     CDS             3791..4312
                     /locus_tag="VM406_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HVL75933.1"
                     /translation="MSDIIKNKAVVADDWTVLRLGENDTPEAVAVPAGKVIVPLKVWQ
                     AQRAALEGRAELGVWLASDERPEDLKDDLARFGVIAIDFPKFADGRGYSLAFLLRSRL
                     GYAGELRAIGDVLRDQLFYMQRVGFDAFAVRADKDIHDAVKSLSDFTEPYQTSWDQKL
                     PLFRRVRRSGVAA"
     gene            4309..5019
                     /locus_tag="VM406_07945"
     CDS             4309..5019
                     /locus_tag="VM406_07945"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HVL75934.1"
                     /translation="MSEQAVLVEQTRATLARIAHEFAPAVFASSLAAEDMVLTDLILR
                     AKLPITIFSLETGRLHKETLAMLDRVRAHYDYEIRLYKPEQSAVDVYVRENGLNAFYD
                     SVEMRKECCRIRKVEPLRRALAGNRAWITGQRRAQSSTRAELAVQEDDPAHGMVKFNP
                     LADWSEQQVWDYIRANEVPYNPLHDRGYPSIGCEPCTRAIQPGEDIRAGRWWWESPES
                     KECGLHVVEGKLVRIKAV"
     gene            5203..6138
                     /gene="cysD"
                     /locus_tag="VM406_07950"
     CDS             5203..6138
                     /gene="cysD"
                     /locus_tag="VM406_07950"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HVL75935.1"
                     /translation="MNTAVEKFFHDQASNRHLDWLESEAIHIMREVAAECTNPALLFS
                     GGKDSVVLLRIAEKAFRPGRFPFPLVHIDTGHNFPEVIEFRDRRVAELGERLIIGSVE
                     DSIRRGTVRLRNPQTDSRNAAQAVTLLETIAEHKFDACIGGARRDEEKARAKERIFSF
                     RDEFGQWNPKAQRPELWDLYNTRVHPGENMRVFPISNWTELDVWQYIAREQLALPSIY
                     YAHQRQVIPRNGLLVPLTELTPPREGETVETRTVRFRTVGDISCTCPVASDATTVDAI
                     IAETAVTQITERGATRMDDQTSEASMEKRKKEGYF"
     gene            6138..7460
                     /locus_tag="VM406_07955"
     CDS             6138..7460
                     /locus_tag="VM406_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HVL75936.1"
                     /translation="MNAPVMDQAISEAGLERGLLRFITAGSVDDGKSTLIGRLLFDSK
                     GIFADQLDAISRAKHKRTVGDTVDLSLLTDGLEAEREQGITIDVAYRYFATPKRKFII
                     ADTPGHEQYTRNMVTGASTADAVIILVDVSKVKFDEAGNAQLLTQTKRHSTIAHLLRI
                     EHVIVAVNKMDLVNYDQHVYERIVTAYRRFADQLGLTDVQAIPLSALAGDNVVTASDR
                     MPWYQGPTLISLLESLSVYEDAHDEPLRFPVQLVARHNGHEANDFRGYMGRIEAGKVR
                     KGDKLVVQPSGQTATVKDILSFDGSLDVARAGQSITLLLDEYLDISRGDMLAAADAPA
                     TPMKTVNADICWLSEEPLDVRRKYWLKHTTRQVAARVAKIDTLLDINTQQRHPADALK
                     LNDIATVAINVQQPLAADAYDAIRATGSFILIDEVTHQTVAAGMIRKG"
     gene            7476..8237
                     /gene="cobA"
                     /locus_tag="VM406_07960"
     CDS             7476..8237
                     /gene="cobA"
                     /locus_tag="VM406_07960"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HVL75937.1"
                     /translation="MCTHASGARPTGKVYLVGAGPGAADLITVRGARILGQAEVVLYD
                     ALVTPEMLALCPQAVKLSVGKRSGQRSTAQAVINEQLVACAQKYRLVVRLKGGDPMMF
                     GRADEELRALEAAGIAFEIVPGITAALAAAASTRQPLTKRGIARSVAFFTSSTAPGHP
                     EQTTLPNCDTLIQYMGGREAITTSQKLIEQGRSPNMPVIVIENCSRANERVMHLRLKD
                     LERGLATCEGPVLVMFGEALETRDGPNEAALPWKR"
     gene            complement(8346..8729)
                     /locus_tag="VM406_07965"
     CDS             complement(8346..8729)
                     /locus_tag="VM406_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HVL75938.1"
                     /translation="MSVPQRALVLFAHGARDPRWAEPFQKLQERVQAARPDAVVATAF
                     LELMSPSLPELAADLANKGCMDVTIVPVFFGQGGHIRRDLPLLVDRLRADHPQVRFHV
                     TGAVGEDAAVLDALARYCADALGRA"
     gene            complement(8726..9853)
                     /gene="lptG"
                     /locus_tag="VM406_07970"
     CDS             complement(8726..9853)
                     /gene="lptG"
                     /locus_tag="VM406_07970"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HVL75939.1"
                     /translation="MRIVDRYVSAQIVGAVLFALIAFLSLFAFFDLIGELPMVGRGGY
                     ALHHAFAYVLLGLPGYVYDLMPIAALIGTIYALAQLAARSEFTIMRVSGLSTSGAWWI
                     LAKVGLVFVLITFLFGEVLAPAASSLAERLKLQAQGASVSQEFRSGLWTKDVIRREGL
                     TGEAIGSRFLNVGDVSPQGQLRGVRMYEFDRDLHLTSTVTAAHADYQGNNVWRLSDVT
                     ETRFDARVADFTAVVSTQKLQARDLVSEITPDILSVLFADPDRMSAYGLARYTRHLAE
                     NNQSTERYEIAFWKKLFYPLAVFVMMALALPFAYLHFRSGGVSLKIFIGVMIGVSFQL
                     INSLFSHLGLLNTWPPLTTAVLPSLFFLAVAMGALTWVQRR"
     gene            complement(9850..10992)
                     /gene="lptF"
                     /locus_tag="VM406_07975"
     CDS             complement(9850..10992)
                     /gene="lptF"
                     /locus_tag="VM406_07975"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HVL75940.1"
                     /translation="MIFQRALRRELTSTAGAVFTTLFTITITVMLIKILGQAAGGRVA
                     SQDVIALIGFAALRYLPIILILTSFISVLVVVTRSYQDSEMVVWFASGQSLTSWIRPV
                     LTFGAPVLAAIIALSFFVTPWANQQAVEFRERFQQREDVARIAPGRFQESSSSDRVFF
                     VEGVAGDASHVRNIFVNTRQNGQNTIVVAREGRVVHDERGDKFLVMDKGRRYDGVDSQ
                     TEFRMMEFERYGVLVARPSQAVVGDLSARSLPTHMLLRDLNQYNLGELMWRISLPIMG
                     LLLMLLAIPLGFVNPRGGRSANLLIALLLYIVYSNMVSVLQASIVQQRISFAMAWWPL
                     HLAAALLILGLFSWRLYVNSPRHPLAMWSAFRRNRKRLPATEAVAR"
     gene            11054..12571
                     /locus_tag="VM406_07980"
     CDS             11054..12571
                     /locus_tag="VM406_07980"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HVL75941.1"
                     /translation="MDFSIKNLDSRTGAAGIKTGCLVVGVFEDKKLSEVAREVDKSGA
                     VTAAVKSGDISGKPGSTLLLRGLAGVAAERVLLVGLGKDEPLGDKNFTSAVQAAGRVL
                     ATLGAADATLALPLDRFARRDAAWGVRAAVLAVREGAYRYDITKSKKDDAPNGVRKFA
                     LLSADAAARAALAEAQAIANGADLTRELGNLPSNVCTPTYLANTAKKLGKDFGFGVEI
                     LERKQIEALKMGSFLSVTKGSEEPPKFIVLKHMGGKAKDAPVVLVGKGITFDTGGISL
                     KPGANMDEMKYDMCGAASVLGTFRALGELNLKMNVIGVIPACENMPSGRASKPGDIVT
                     SMSGQTIEILNTDAEGRLILCDALTYVERFKPAAVVDIATLTGACVTALGHHNTGLFT
                     RDDEAHDALADELLSAGKASGDTAWRMPLGELYQEQLKSNFADMANIGGPPGGAITAA
                     CFLERYTKNYTWAHLDIAGTAWKGGAAKGATGRPVPLLTTFLINRARALGGKKAA"
     gene            12568..13332
                     /gene="xth"
                     /locus_tag="VM406_07985"
     CDS             12568..13332
                     /gene="xth"
                     /locus_tag="VM406_07985"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HVL75942.1"
                     /translation="MKLATWNVNSLKVRLPQVLQWLAENPVDVLCIQETKLTDDKFPQ
                     AEIEAAGYASVFTGQKTYNGVAILSKHPFSEVVRNNPLFEDEQQRIIGATIEGMRVVC
                     AYVPNGQAVGSDKYQYKLRWLGALREWLGEELKTHENLALLGDYNIAPDERDVHDPAA
                     WEGQVLFSEPEREHFRELLALGFKDAFRLFEQPEKTFSWWDYRMMAFRRNMGLRIDHI
                     LLSAPLAARCSACAIDRAPRKWEQPSDHAPVVATLD"
     gene            complement(13689..14897)
                     /locus_tag="VM406_07990"
     CDS             complement(13689..14897)
                     /locus_tag="VM406_07990"
                     /inference="COORDINATES: protein motif:HMM:NF021504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HVL75943.1"
                     /translation="MNFPTLLKWFETRPSSKPAPPPAATPIDKPRAQIRVGIPRVLNI
                     WSTHQFWVGFLTALGIEPNKIDFSSDTSEEQARSFAKGRGTVDCCYPVKCMSGHYGEL
                     LMRKKHRKIDILLSPMIYSLPSILQGQVLNTIACPRVMAAPENIKSGFMKEQDAFAEA
                     GVRYVSPLVYLGEPELVPKQMYEALKDVLPGLTPTETRAAVEQGYAALRAFNERARRR
                     SRDVLEQCARQQRPCLLVLARPYHMDPGIGHEIEVDLQAYGYPILWAQYLPVDADLMQ
                     WVFGDDMREGHTRSPFDIGDVWPSSYSSNTNEILWGAKFAARMPWIAGVIRLASYECG
                     MDQPTYTPVQQIIERSGTLFFSFQELDSTKPAGSVKIRVETIAHYLEKYAQSIIERKL
                     SASRPGCPLG"
     gene            complement(14815..>16033)
                     /locus_tag="VM406_07995"
     CDS             complement(14815..>16033)
                     /locus_tag="VM406_07995"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HVL75944.1"
                     /translation="ALYRPKGKTEPAAPAKVVERPVFLGCDFGSTTAKAVVLSAEREL
                     LFSAYVMSKGNPIEDAQGILRQVKEAGYASIAALAITGYGKDLLHDIVGSDVAIVETV
                     AHATAALHFFPDADVICDVGGCDVKIMLLRQGTVSDFRLNSQCSSGNGAFLQGVAERY
                     DIPLEQYADLAFKAKSMPTLAMGCGVFLQSDIVNQQRKGWAAEEIMASLAAVLPLNVW
                     VYAGQLQNLAAAGRKFVLQGGTHRNLAVVKAQVQFVREKVPDAEIVVHPHPGEAGAIG
                     AALCAADWAARGSATRFRGFETIEALVHRSTTNEDTTCRWCPVNCRRTFIDVQLPGAQ
                     GRPWSKVPVEEGWERVISGNSCPKGLLEDASEMRIVKARLEESKREFPNIAEMVRDQA
                     FKQARATAGSDAD"
BASE COUNT         3044 a         5250 c         5144 g         2595 t
ORIGIN      
        1 ggcttttcct ttggaggttt caaatgtgga aaagcccttc gccgaacacc aagggttgcg
       61 gtcttggctt cggcgaaggg ttctctgctc cggggaccgg aacggcagat agtgtaatcc
      121 gcgattcgcg tccgtgcgga aagttcctgc gcttcgcgcg ccgacgtcag aacataccgc
      181 cgacgttgaa cagcgtggcc acgccgagca gcgcaaacag cacggccgcc acgccatgca
      241 ccaccttcat cgacaccatc cgcgttaccc ggtctccgag caggacggcc ggcacgttgg
      301 cgatcatcat gcccagcgtg gtcccgatca cgaccgcacc gaaagcgtcg aacttggcgg
      361 caagcgcaac ggttgcgatc tgggtcttgt cgcccatctc cgccaggaag aatgccgcgg
      421 tggtcacacc gaacacaccg gctgccgccg gtttcacatc gacgtcgtcg agcttgtccg
      481 gaaccatcac ccacaccgcc atcgccagga aggaaatcgc gaggatccag cgcaacacgt
      541 ccgggccgag ccagtcggaa agcaatgcgc cgagcgcgcc ggccagcgcg tggttcagca
      601 gcgtggcgac aaagatgccg aggatgatgg gcagcggccg ccggaagcgc gcggccagca
      661 gcagggacag cagttgcgtc ttgtcgccca tttcggcgag cgcgacgata cctgtggaaa
      721 gaagaaaggc ttccatgaat gctccggggc cggatccgca ccaatgacca tgcgcgccct
      781 ccggcccacc ggaagcggca tggtcaaagg tctcgccaaa cccgaagtcg cttgcgccat
      841 ggccgccggg cggcagccaa gtctgttgac gcaagcccct ctcgctgatc atgcagatcg
      901 gagggaggct actccccaat gacaggcggc attgtaccgg atcaacccgg caggatgctt
      961 agtggaaaga aagctacagt tcgtagctgt ctttctcccc gctcattacc tgctcgacga
     1021 gcttgcgcgt gagcgtgggc gacagcagtt cgatgaagct gtatacgtag cttcgcaggt
     1081 aggccccctg cttcagcgcg acgcgcgaga catttgtgcc gaacaggtgg ccgaccgata
     1141 tcgcgcgcaa tcccttgtcg cgctcgggat caaacgccat gccggcgatg atgccgacac
     1201 ccatgcccaa ctccacgtag gtctttatca cgtcggcatc gatcgcttcg agcagcacgt
     1261 ccggcttcag atgccggatc tgaaacgcat ggtcgatctt cgcccggccc gtgaaggcgc
     1321 tgtcataggt gatcaacggg tagcgggcga tgtcttccag cgtgatcgga ttcgccttct
     1381 gcaacgggtg gtccggcggc accacgacca cgtgctccca ctggtagcac ggcagcgaaa
     1441 tcaggccttc ggcatcggcg atgaattcgg tggcgatggc aaggtccgcc tggtcgcgct
     1501 ggaccatctc ggtgacctgc ttgggattgc cctggagcag ggacaggcgc actttcggat
     1561 acttctgcgt gaacgcctgc acgactttgg gcagcgcata gcgcgcctgc gtatgcgtgg
     1621 ttgcgatcgt gaaactgccg gtatcctggg ccgcgaattc cttgccgatc tgcttgagcc
     1681 cttcgatttc ctgcatgatc agctcgaccg agcgcaacac tgccctgccc ggctcggtca
     1741 gcccgcggat gcgcttgccg tggcgggtga agatatcgac tcccagctct tcctccagct
     1801 cgatgatcgc cttcgacacg cccggctggg acgtatacag cgcctttgcc gcctcagtga
     1861 ggttgaaatt ctggcgcacg gcttcccgca cgaagcggag ttggtggaga ttcattgcct
     1921 ttcctgttca gccctgcata tgccccgagg gcatataagc aaataaatac taagtagttt
     1981 ggaataaggg tcaagtttat tacgatcggg ctactttgcg gaggcgaagg gttcgcccgc
     2041 agcttccccc cggcgcggca gcctgccgtt acttgccgga ctttcaagat tggaccccga
     2101 ctgatgtacc gttacgacga atacgaccac cagatcatcc gggaacgggt tgcgcagtac
     2161 cgcgaccagg ttcgccgccg cctgtccggc gaactgaccg aagacgaatt ccgcatcctg
     2221 cgcctgcaga acggcttgta ctaccagcgc cacgcctaca tgctgcgcgt ggcgattccg
     2281 tatggcctgc tgtcgtcgaa gcaggtgcgc atgctggcgc atatcgcgcg cgactacgac
     2341 cgcggctacg gccacttcac cacgcgccag aacatccagt acaactggat ggagctggag
     2401 cagaccccgg aaatcctgga gaagctggcc tcggtcgaga tgcatgcgat ccagacctcg
     2461 ggcaactgca tccgcaacat cacttccgac ccctatgccg gcatcgcccc ggacgagatc
     2521 gttgatccgc gcccctactg cgagatcctg cgccagtgga gcaccttcca tcctgagttc
     2581 gcctacctgc cgcgcaagtt caagatcgcg atcaacggtg cggcagaaga ccgtgccgcg
     2641 atcatggtgc acgacatcgg cctgacggtg ctgaagaacg aagccggcga agtcggcttc
     2701 cgcgtgttcg tcggcggcgg catgggccgc acgcccatcc tcggcagcga gatccgcgag
     2761 ttcctgccgt ggcagcacgt gttgagctac atcgaagcca tcgtgcgcgt ctacaaccag
     2821 tacggccgcc gcgacaacat ctacaaggcg cgcatcaaga tcctcgtcaa ggcgatcggc
     2881 atcgaggaat tccgccgcca ggtcgaagag gagtggcaac agatcaagga cggcccgagc
     2941 accctcacgc aggaggagct ggaccgcgtg gccgcgcatt tcaccgcccc ggcctatgaa
     3001 gcgctgccgg ccgacgatgc gaagctcgac gccttccagg ccgagaacaa ggcgttcgcg
     3061 aactgggtca agcgcaatgt gaaggcgcac cgcgtgcccg gctatgcggc cgtcgtgctg
     3121 tcgctgaaga agaccggcgt gcccccgggc gacgccaccg acgagcagct cgacttcgcc
     3181 gccgacatcg ccgagcgcta cagcttcggc gaactgcgcg tcacgcacga gcagaacctg
     3241 gtgctgtcgg atgtgaagaa gtccgatctg atcgcgctgt gggaagagat caaggcgcaa
     3301 ggcctggcca ccccgaacat cggcctgctg accgacatga tctgctgccc gggcggcgat
     3361 ttctgcgcgc tggcgaacgc gaagtcgatc ccggtcgcgg gcgagatcgc cgagcgcttc
     3421 gacaacctcg acttccagca tgacatcggc gagatcgaac tgaatatctc gggctgcatc
     3481 aattcctgcg gccaccacca tgtcggccac atcggcatcc tcggcgtcga caaggacggc
     3541 agcgagtggt accagatttc ggtgggcggc gcccagggca acgcgtcctc gatcggcaag
     3601 atcatcgggc cgtcgttctc cgcgccgcag gtgccggagg tgatcgaccg cctgctcaag
     3661 gtctacgtgc gcgagcgcca cgaggacgag cgattcatcg acaccgcgcg ccgcatcggc
     3721 atcgcgccgt tcaaggaata cgtgtacgcc actcccatca agaccgaaca aaacgccgca
     3781 gaggacgccc atgtctgaca tcatcaagaa caaggcggtc gtcgccgacg actggaccgt
     3841 gcttcgcctg ggcgagaacg acacgcccga ggccgtggct gtcccggcgg gcaaggtgat
     3901 cgttccgctg aaggtctggc aggcgcagcg cgccgcgctg gagggacggg cggaactggg
     3961 tgtgtggctg gcgagcgacg agcgccccga ggacctgaag gacgatctgg cccgctttgg
     4021 cgtgatcgcg atcgacttcc cgaagtttgc cgacggccgc ggttactcgc tcgccttcct
     4081 gctgcgctca cgcctcggct atgccggcga actgcgcgcg atcggcgacg tgctgcgcga
     4141 ccagctgttc tacatgcagc gcgtgggctt cgacgcgttc gcggtgcgcg cggacaagga
     4201 catccatgac gcggtcaaga gcctgtcgga tttcaccgag ccgtaccaga cgtcgtggga
     4261 ccagaaactg ccgctgtttc gccgtgtcag gcgctcgggg gtcgccgcgt gagcgaacag
     4321 gccgtcctgg tcgagcagac ccgtgcgacg ctcgcgcgca ttgcgcacga gttcgcgccc
     4381 gccgtgttcg cctccagcct cgcggcggaa gacatggtgc tgaccgacct gatcctgcgc
     4441 gcgaagctgc cgatcacgat cttctcgctg gagacgggac gcctgcacaa ggaaacactg
     4501 gcgatgctcg atcgcgtgcg cgcccactac gactacgaga tccggctgta caagccggaa
     4561 cagtccgcgg tggacgtcta cgtgcgggag aacggcctga acgcgttcta cgacagcgtc
     4621 gaaatgcgca aggaatgctg ccgcatccgc aaggtcgagc cgttgcggcg cgcgctggcg
     4681 ggcaaccgcg cctggatcac cggtcagcgc cgcgcgcagt cgagcacgcg tgccgaactg
     4741 gcggtgcagg aggatgaccc ggcccatggc atggtcaagt tcaatccgct tgcagactgg
     4801 tcggagcaac aggtgtggga ctacatccgt gcgaacgagg tgccgtacaa cccgctgcac
     4861 gaccgcggct acccgtcgat cggctgcgaa ccctgcacgc gcgcgatcca gccgggcgag
     4921 gacattcgcg cgggccgctg gtggtgggaa tcgccggagt ccaaggaatg cggcctgcat
     4981 gtcgtcgaag gcaagctggt gaggatcaag gcggtctgaa agccgactgc tgcggcaacg
     5041 actcacgtgc tgtcatcccg agcgcagcga cggatctccg cgcacctgga gcgcatggcg
     5101 tgaaagaaga gcagagcttc tcgtttcacc tggaatgaca gtctctcgac agtcatgcaa
     5161 ccgccccgat aaacatattc gtacttgcac cacttcgaac ccatgaacac cgcagtcgaa
     5221 aaattcttcc atgaccaggc cagcaaccgt catctcgact ggctggaatc cgaggcgatc
     5281 cacatcatgc gcgaggtcgc ggccgagtgt acgaacccgg cgctgctgtt ctcgggcggc
     5341 aaggattcgg tcgtgctgct gcgcatcgcc gagaaggctt tccgccccgg caggtttccg
     5401 ttcccgctgg tgcacatcga taccggccat aacttcccgg aagtgatcga attccgcgac
     5461 cgccgcgtcg ccgaactggg cgagcgcctg atcatcggct ccgtcgaaga ctcgatccgg
     5521 cgcggcaccg tgcgcctgcg caatccgcag accgattccc gcaatgccgc gcaggcggtc
     5581 acgctgctgg agacgatcgc cgagcacaag ttcgacgcct gcatcggcgg tgcgcgtcgt
     5641 gacgaggaaa aggcgcgcgc gaaggagcgc atcttctcgt tccgcgatga attcggccag
     5701 tggaatccga aggcgcagcg ccccgaactc tgggacctgt acaacacccg cgtgcatccg
     5761 ggcgagaaca tgcgcgtatt cccgatctcc aactggaccg agctcgacgt ctggcaatac
     5821 atcgcccgcg aacagctcgc cctgccgtcg atctactacg cgcaccagcg ccaggtgatc
     5881 ccgcgcaacg gtctcctggt gccgctcacc gaactgacgc cgccgcgtga gggcgagacg
     5941 gtcgagacgc gcaccgtgcg ctttcgcacc gtcggcgaca tctcctgcac ctgcccggtc
     6001 gcctcggacg cgacaacagt ggacgcgatc atcgccgaaa ccgcggtgac ccagatcacc
     6061 gaacgcggcg cgacccgcat ggacgaccag acgtctgagg catcgatgga aaaacgcaag
     6121 aaggaaggat atttctgatg aacgcgccag tcatggacca agccatatcc gaagccgggc
     6181 tggagcgcgg cctgctgcgc ttcatcaccg ccggctccgt ggacgacggc aagagcacgc
     6241 tgatcggccg cctgctgttc gacagcaagg gcatcttcgc cgaccagctc gatgcgatct
     6301 cgcgcgccaa gcacaagcgc acggtcggcg acaccgtcga tctttctctg ctcaccgacg
     6361 ggctggaagc cgagcgcgag caaggcatca caattgacgt cgcgtaccgc tacttcgcga
     6421 cgcccaagcg caagttcatc atcgccgaca cgccgggcca cgagcagtac acgcgcaaca
     6481 tggtcaccgg cgcctccacc gccgacgcgg tgatcatcct ggtcgacgtg tcgaaggtga
     6541 agttcgacga ggccggcaat gcgcagctgc tgacccagac caagcgccac tcgacgatcg
     6601 cccatctgct gcgcatcgaa catgtgatcg tcgcggtgaa caagatggac ctggtgaact
     6661 acgaccagca cgtgtatgaa cgcatcgtca ccgcctaccg caggttcgcc gaccagctcg
     6721 gactgaccga cgtgcaggcg attccgctgt cggctctggc cggcgacaat gtggtcacgg
     6781 ccagcgacag gatgccgtgg taccagggcc cgacgctgat cagcctgctc gaatcgctgt
     6841 cggtctacga ggatgcgcac gacgagccgc tgcgcttccc ggtgcagctg gtcgcccgcc
     6901 acaacggcca cgaagccaat gacttccgcg gctacatggg ccgcatcgaa gccggcaagg
     6961 tcagaaaggg cgacaagctg gtcgtgcagc cgagcggcca gacggccacc gtgaaggaca
     7021 tcctgagctt cgacggctca ctcgatgtcg cccgtgccgg gcagtcgatc acgctgctcc
     7081 tggacgagta cctggacatc tcgcgcggcg acatgctggc cgccgccgac gcgccggcca
     7141 cgccgatgaa gacggtcaac gccgatatct gctggctgtc ggaggagccg ctcgacgtgc
     7201 ggcgcaagta ctggctcaag cacacgacgc gccaggtggc ggcgcgggtg gcgaagatcg
     7261 ataccctgct cgacatcaat acccagcagc gccatccggc cgacgcgctc aagctcaacg
     7321 acatcgcgac ggtagcgatc aatgtccagc aaccgctggc ggccgatgcc tacgacgcga
     7381 tccgcgcaac gggctccttc atcctgatcg atgaagtcac ccaccagacg gttgcggcag
     7441 gtatgatccg caaaggctga ctccacctcc ccgcaatgtg cactcacgct tccggcgccc
     7501 gcccgaccgg caaggtctac ctggtgggcg ccggccccgg cgccgccgac ctgatcaccg
     7561 tgcgcggcgc acgcatcctc gggcaggccg aggtcgtgct ctacgacgcg ctggtcaccc
     7621 ccgagatgct cgcgctgtgt ccgcaggcgg tgaagctctc agtgggcaaa cgctccggac
     7681 agcgctcgac cgcccaggcg gtaatcaacg aacaactggt cgcgtgcgcg cagaaatacc
     7741 ggctggtggt acgcctgaag ggcggcgatc cgatgatgtt cgggcgcgcg gacgaggaat
     7801 tgcgcgcttt ggaggcggcc ggcatcgcgt tcgagatcgt ccccggaatt accgccgccc
     7861 ttgccgccgc ggcgtcgacc aggcagccgc tgaccaagcg cggcattgcg cgcagcgtcg
     7921 ccttcttcac ctcaagcacg gcgccgggac accccgagca gaccacgctg ccgaattgcg
     7981 acacgctgat ccagtacatg ggcggacgcg aggcgatcac cacctcccag aagctgatag
     8041 aacagggccg ctcgccgaac atgccggtga tcgtgatcga gaactgcagc cgcgccaacg
     8101 aacgcgtgat gcatctgcgc ctgaaggacc tggagcgcgg actggcgacc tgcgagggcc
     8161 cggtgctggt gatgttcggc gaggcgctgg agacgcggga cggaccgaac gaggcggcat
     8221 tgccctggaa acgttagcgc gacgccgatg gcatcgcgcg ccggctcgga ccgcgcggat
     8281 gcacgaccgg cgcgggattg ccagcgtctg cagccgcagt tcgacgcccg ggaagtgcga
     8341 agcgttcagg cgcgcccaag ggcgtcggcg cagtagcgcg cgagcgcatc gagcacggca
     8401 gcatcctcgc cgacggcgcc ggtgacgtgg aagcgcacct gcggatgatc ggcgcgcagc
     8461 cggtcgacga gcagcggcag gtcgcggcgg atgtggccgc cttggccgaa gaacaccggc
     8521 acgatggtga cgtccatgca tcccttgttc gcaagatcgg ccgcaagttc gggcagggac
     8581 ggactcatca actccagaaa ggccgtcgcc accaccgcgt ccgggcgggc cgcctgtacg
     8641 cgttcctgca gcttttgaaa cggctcggcc cagcgcggat cgcgcgcgcc atgggcgaac
     8701 aggacgaggg cgcgctgcgg cacgctcatc gccgctgaac ccatgtgagc gcccccattg
     8761 cgaccgcgag gaagaacagg ctgggcagta ccgccgtcgt cagcggcggc caggtattga
     8821 gcagcccgag gtgagagaac aggctgttga tcagctggaa gctcaccccg atcatcacgc
     8881 cgatgaaaat cttcaggctg actccgccgg agcggaaatg cagataggca aacggcaggg
     8941 ccagcgccat catcacgaac accgccagcg gatagaacag ctttttccag aacgcgatct
     9001 cgtagcgctc ggtgctctgg ttgttctcgg caaggtggcg ggtgtagcgc gccaggccgt
     9061 aggccgacat gcggtccgga tcggcgaaca gcaccgacag gatatccggc gtgatctcgg
     9121 aaacgagatc gcgcgcctgc agtttctggg tcgacacgac cgccgtgaag tcggccacgc
     9181 gggcgtcgaa gcgggtttcg gtcacgtccg acaggcgcca gacgttgttg ccctggtaat
     9241 ccgcatgggc cgctgtgacg gttgaggtca ggtgcaggtc gcggtcgaac tcgtacatgc
     9301 gtacaccgcg cagctgtccc tgcggcgaca cgtcgcccac attgagaaag cgcgaaccga
     9361 tcgcctcgcc ggtcagacct tcgcgccgga tcacgtcctt ggtccacagc ccggagcgga
     9421 attcctggga cacggaagcg ccctgcgcct gcagcttcag gcgttcggcc agcgaactcg
     9481 cggccggcgc cagcacttcg ccgaacagga aggtaatcag cacgaacacc aggccaacct
     9541 tggccagaat ccaccatgcc cctgaagtcg acaggccgga cacccgcatg atggtgaact
     9601 cggaacgtgc ggcaagctgg gcgagcgcgt agatggtgcc gatcaatgcg gcgatcggca
     9661 tcaggtcgta cacgtagccg gggaggccga gcagaacgta ggcgaacgcg tggtgcagcg
     9721 catagccgcc acggccgacc atcggcagct cgccgatcag gtcgaagaag gcgaacagcg
     9781 acaggaaggc gatcagcgca aacagcaccg cgccgacgat ctgtgccgat acatagcggt
     9841 cgacgattct catcgcgcaa ccgcctccgt ggccggcagg cgcttgcggt tgcggcggaa
     9901 tgcgctccac atcgcaagcg ggtggcgcgg actgttcacg tacaggcgcc aggaaaacag
     9961 accgaggatc agcagcgccg cggccaggtg cagcggccac cacgccatcg caaagctgat
    10021 gcgctgctgg acgatcgagg cctgcagcac gctgaccatg ttgctgtaga cgatgtacag
    10081 gagcagcgcg atcagcagat tggccgagcg gccgccgcgc gggttcacga agcccagcgg
    10141 aatggcaagc agcatcagca acagacccat gatcggcagc gagatgcgcc acatcagttc
    10201 gcccaggttg tactggttca ggtcgcgcag cagcatatgg gtcggcaacg agcgcgcgga
    10261 aagatcgccc accacggcct gggacgggcg cgcgaccagc acgccgtaac gctcgaactc
    10321 catcatgcgg aactcggtct ggctgtcgac gccatcatag cggcgcccct tgtccatcac
    10381 gaggaacttg tcgccgcgtt cgtcatggac gacgcgcccc tcgcgcgcga cgacgatcgt
    10441 gttctggccg ttctggcgcg tgttgacgaa gatgttgcgc acatggcttg catcgccggc
    10501 cacgccttcc acgaagaaaa cccggtcgga cgacgaggac tcctggaagc ggccgggcgc
    10561 gatgcgggcg acgtcctcgc gctgctggaa gcgttcgcgg aactcgaccg cctgctggtt
    10621 cgcccatggt gtgacgaaga agctcagcgc gatgatggcc gcgaggacgg gcgcgccgaa
    10681 cgtgagcacg ggcctgatcc agctggtgag gctttgtccg gatgcgaacc agacaaccat
    10741 ttcggagtcc tgataggaac gtgtcacgac caccagcacc gagatgaagc tggtgaggat
    10801 caggatgatg ggaaggtaac gaagcgcggc gaaaccgatc agggcgatca cgtcctgcga
    10861 agccacgcga ccgccggcgg cctgaccaag tatcttgatc agcatgacag tgatcgtaat
    10921 cgtgaacagc gtggtgaaga ccgcgcccgc ggtgcttgtc agttcgcggc gtagagcgcg
    10981 ctgaaagatc attgatttga cgactataat gagcggcttt ggcgcctgct acaggccccc
    11041 gtaggagcga cgcatggact ttagcataaa aaatctggac tccaggaccg gcgcggccgg
    11101 catcaagacc ggctgcctcg tggtcggtgt ctttgaggac aaaaagctgt cggaagttgc
    11161 acgcgaagtc gacaaatctg gcgccgtcac ggccgcggtg aaaagcggcg acatcagcgg
    11221 caagcccggc tcgacgctgc tgctgcgcgg gttggccggc gtggccgccg agcgcgtgct
    11281 gctggtcggg ctcggcaagg acgagccgct cggcgacaag aacttcacct cggcggtcca
    11341 ggctgcaggc cgcgtgctcg ctacgctggg cgccgccgat gcgaccctgg cgctcccgct
    11401 ggaccgcttc gcccggcgcg atgccgcatg gggggtgcgc gcggcggtgc tggcggtgcg
    11461 cgaaggcgca taccgttatg acatcaccaa gagcaagaag gacgacgcgc ccaatggcgt
    11521 gcgcaagttt gccctgctga gcgcggatgc cgcggcccgg gccgcactgg ccgaggcaca
    11581 ggcgattgcc aacggcgccg acctgacccg tgaactgggc aacctgccga gcaacgtctg
    11641 cacgcccacc tacctggcaa acaccgcgaa gaagcttggc aaggatttcg gcttcggggt
    11701 cgagatcctc gagcgcaagc agatcgaggc gctgaagatg ggcagtttcc tgtccgtcac
    11761 caagggcagc gaagaaccgc cgaagttcat cgtcctgaag cacatgggcg gcaaggccaa
    11821 ggacgcgccg gtggtcctgg tcggcaaggg catcaccttc gacaccgggg gcatctcgct
    11881 caagcccggc gccaacatgg acgagatgaa gtacgacatg tgcggcgcgg cctcggtgct
    11941 gggcaccttc cgcgcactcg gcgaactgaa tctgaaaatg aacgtgatcg gcgtgattcc
    12001 ggcctgcgag aacatgccgt cggggcgcgc gtccaagccg ggcgacatcg tcacctcgat
    12061 gtccggccag accatcgaaa tcctgaacac cgacgccgag ggccgcctga tcctgtgcga
    12121 cgccctcacc tacgtcgagc gcttcaagcc ggccgccgtg gtcgatatcg ccaccctcac
    12181 cggcgcctgc gtgaccgcgc tcggccatca caacaccggc ctgttcacgc gcgacgacga
    12241 ggcccatgac gcgctcgccg acgaactgtt gagcgcaggc aaggcgagcg gcgacaccgc
    12301 atggcgcatg ccgctgggcg agctgtacca ggagcaactg aagtccaact tcgccgacat
    12361 ggccaacatc ggcggcccgc ccggcggcgc gattaccgca gcctgcttcc tcgagcgcta
    12421 cacgaagaac tacacctggg cgcacctgga catcgccggc accgcgtgga agggcggagc
    12481 cgccaagggt gcgaccggcc gtccggtgcc gctgctgacg accttcctga tcaatcgcgc
    12541 ccgcgccctt ggcggcaaga aggctgcatg aaactcgcca cctggaacgt caactcgctg
    12601 aaggtccgcc tgccgcaggt gctgcaatgg ctggcggaaa acccggtcga cgttctctgc
    12661 atccaggaaa ccaagctcac cgacgacaag ttcccgcagg ccgagatcga ggctgccggc
    12721 tatgcgtcgg tatttaccgg gcagaagacc tacaacggcg tggcgatcct gtcgaagcat
    12781 ccgttctcgg aggtggtgcg caacaacccg ctgttcgagg acgagcagca gcgcatcatc
    12841 ggcgcgacca tcgaagggat gcgcgtggtg tgtgcatatg ttccaaacgg gcaggccgtg
    12901 ggttcggaca agtaccagta caagctgcgc tggctgggcg cgctgcgcga atggctgggc
    12961 gaggaactga agacgcacga gaacctggcg ctgctgggcg actataacat cgcaccggac
    13021 gagcgcgacg tgcacgatcc ggccgcgtgg gaagggcagg tgctgttttc ggagccggag
    13081 cgcgagcact tccgagagtt gctggcgctc ggtttcaagg atgcgttccg gttgttcgag
    13141 cagccggaga agaccttcag ctggtgggac taccggatga tggctttccg ccgcaacatg
    13201 ggcctgcgca tcgaccacat cctgctctcg gcgccgctgg cggcgcgctg cagcgcctgc
    13261 gcgatcgacc gcgcgccgcg caagtgggag cagccgtcgg atcacgcgcc ggtggtggcg
    13321 acgctggatt agcggtgggt tgtcgagggt tcgtactccg tacgctgtcg acccgtcgtc
    13381 ctgtcattcc cgctgtcctg ccatccccgt cgttccgtca ttccctctga cctgtcatcc
    13441 cgaacgcagt gagggatctc gcgcctgaac gggtggcgtc gattctcgag cggatgagct
    13501 tcctcgttgc attcggaacg acaggcaggg gcggcgaccg gcgccgctcc cccgctgtcc
    13561 tgtcatcccg aacgcagtga gggatctcgc gcctgaacgg gtggcgtcga tgctcgagcg
    13621 gatgagcttc ctcattgcat tcggaacgac aggcaggggc agtgcaggtg cccctccttg
    13681 cgacgccctc agcccagcgg acaaccgggc cggcttgccg acagcttgcg ctcgatgatc
    13741 gactgcgcgt acttttccag gtaatgcgcg atcgtctcga ccctgatctt caccgagccg
    13801 gccggcttgg ttgaatcgag ttcctggaac gagaagaaca gcgtgccgga ccgttcgatg
    13861 atctgctgga ccggcgtgta ggtcggctgg tccatcccgc actcatagct ggcgagtcgg
    13921 atcacgccgg ctatccatgg catgcgcgcg gcgaacttcg cgccccacag gatttcattg
    13981 gtgttcgaac tgtacgacga cggccagaca tcgccgatgt cgaatggcga gcgggtatgg
    14041 ccttcgcgca tgtcgtcgcc gaacacccat tgcatcaggt ccgcgtcgac cggcaggtac
    14101 tgggcccaca ggatcggata gccgtaggcc tgcaggtcga cctcgatttc atggccgatt
    14161 ccgggatcca tgtgatacgg gcgcgccagc accagcaggc agggcctctg ctggcgcgcg
    14221 cactgttcca gcacgtcgcg cgagcgcctg cgcgcacgct cgttgaacgc gcgcagcgcg
    14281 gcgtagccct gttcgaccgc cgcgcgcgtc tctgtagggg tcaagccggg caatacatcc
    14341 ttcaaggcct cgtacatctg cttcggcacc agctccggtt cgcccagata cacgagcggc
    14401 gacacatagc gcacgccggc ctcggcgaac gcatcctgtt ccttcatgaa gccggacttg
    14461 atgttctccg gcgccgccat cacgcgcggg caggcgatgg tgttcaacac ctggccctgc
    14521 aggatagacg gcagcgaata gatcataggc gacagcagga tgtcgatctt gcggtgtttc
    14581 ttgcgcatca gcagctcgcc gtagtggccg gacatgcact tgaccggata gcagcagtcc
    14641 acggtgcccc gccccttcgc gaacgaacgc gcctgttcct ccgaggtatc ggacgaaaaa
    14701 tcgatcttgt tcggctcgat gccgagcgcg gtcaggaagc cgacccagaa ctggtgggtc
    14761 gaccagatgt tgagcacgcg cggaatgccg acccggatct gggcgcgcgg cttgtcaatc
    14821 ggcgtcgctg ccggcggtgg cgcgggcttg cttgaaggcc tggtctcgaa ccatttcagc
    14881 aatgttggga aattcacgct tgctctcctc cagcctggct ttgacgatgc gcatctcgct
    14941 cgcgtcttcc agcaggccct tcggacagga gttgccgctg atgacacgct cccatccctc
    15001 ctccaccggc accttgctcc acggccgccc ctgcgcgccg ggcagctgga catcgatgaa
    15061 ggtgcggcgg cagttgaccg gacaccagcg gcaggtggtg tcttcattgg tggtcgagcg
    15121 gtgcaccagc gcctcgatcg tctcgaagcc gcgaaagcgc gtggcgctgc cgcgcgcggc
    15181 ccagtcggct gcacatagcg ccgccccgat cgcgcccgcc tcgcccggat gcggatgcac
    15241 gacgatctcg gcatccggca ccttctcgcg cacgaactgc acctgcgctt tgaccaccgc
    15301 gagattgcga tgggtgccgc cctgcagcac gaacttgcgc ccggccgccg ccaggttctg
    15361 cagctgcccg gcatagaccc acacattcag cggcagcacc gccgccagcg aagccatgat
    15421 ttcctcggcg gcccagccct tgcgttgctg gttgacgatg tccgactgca ggaacacgcc
    15481 gcagcccatc gccagcgtcg gcatggattt ggccttgaac gccaggtccg cgtactgttc
    15541 gagcgggatg tcgtagcgct ccgccacgcc ctgcaggaag gcaccgttgc cggacgagca
    15601 ctgggaattg agccggaaat ccgacaccgt gccctggcgc agcagcatga tcttcacgtc
    15661 gcagccgccg acgtcgcaga tcacgtccgc gtcgggaaag aaatgcagcg ccgcggtcgc
    15721 gtgcgcgaca gtctcgacga tggccacgtc cgagccgacg atgtcgtgca gcagatcctt
    15781 gccgtaaccg gtgatcgcca gcgccgcaat cgatgcgtaa cccgcctcct tcacctggcg
    15841 caggatgccc tgcgcatcct cgatggggtt ccctttcgac atcacatagg cggagaaaag
    15901 cagctcgcgc tcggccgaca gcacgactgc cttggccgtc gtgctgccga aatcgcagcc
    15961 gaggaagacc ggacgttcga ccactttcgc aggcgcggcc ggctctgtct tccccttggg
    16021 ccggtacagc gcg
//
LOCUS       DATLGF010000264         5095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_211594, whole genome shotgun sequence.
ACCESSION   DATLGF010000264 DATLGF010000000
VERSION     DATLGF010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211594"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VM406_08000"
     CDS             complement(<1..519)
                     /locus_tag="VM406_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HVL75945.1"
                     /translation="MLHLTDDMIERLVSASEAESVLRSAFIDFAGGRAAMQARARTEA
                     GGVKLSTLGAVIPGQGVTGAKVYTTINGQFRFVILLFSTEDGRPLACLDAGTITRIRT
                     AACSLLAARELAPERPQRMALFGAGVQGSAHAEQFSRAFGLGRIAVCDPYAKPETIAL
                     LQSRCEAEVDVAS"
     gene            complement(563..1273)
                     /locus_tag="VM406_08005"
     CDS             complement(563..1273)
                     /locus_tag="VM406_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HVL75946.1"
                     /translation="MSTSSPSAVVAERADVKLGEQHRPLSAVIRDTLRARILSGEFST
                     GDRLVEGRLSEELGASRVPVREALRALASEGLVTIEPRRGASVVFLSDEMAHDLVEVR
                     ATLEGLNAKLAAQRRTDKVVAELKEVLERGMAAAKTGRPDQLAALNYEFHELLATAAG
                     NAVLTDMVRSLRTRTAMLFAPNTKERARQDWEEHAQVLHAVISGNAELAALLAAQHVH
                     NSAEARMKSRRTAREKTK"
     gene            1430..2896
                     /locus_tag="VM406_08010"
     CDS             1430..2896
                     /locus_tag="VM406_08010"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HVL75947.1"
                     /translation="MDDPDRRFAIGSVSMIATGHPLAAAAARHALAEGGSAVDAAVAA
                     DAVLGVVEPMATGIGGDLLAMIVEPDGQAVAYNGTGRAPASLSAERVAQMPGGRIPER
                     HPWSVTVPGAVCGWHDLHQRYGRLEWPRLLRDAITLARTGFAVAPVAAREWKLFEPVL
                     KADPTAAALYRHGRVPAAGEVFANPELAEFLARIARDGYRAFYDGSAAEAMAAAVRAR
                     GGLLGADDLAAHAGNFCVPLRGEFCGVTVLECPPNTHGCAVIDALLSLEAFAREEGTP
                     QAVAALVEATGRALQTASKTVADPSGNTVCTVIVDRDGLAITLMSSIFKRFGSAIAVP
                     GLGFVLQNRGFGFSAPGHPNGPAGGKRPYHTVIPAACLREGRFHAGLGVVGGLMQPQG
                     QVQLLTRLFAWGEPLREAIAAPRWRLEGGRALALETGFPADAAALLRERGFGEPAAGE
                     GDLAGRSDFGGAQVVLRLPDGSLAGASDPRKDGVALAD"
     gene            3102..3824
                     /locus_tag="VM406_08015"
     CDS             3102..3824
                     /locus_tag="VM406_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HVL75948.1"
                     /translation="MTALMNREEFRAALERAIQGKTATKSPFSVAWANGTLSRAHLAH
                     WAENHYHYVGPFADYLAYVYARMPDSYTEAKDFLLYNMYEEEVGGDRHTDLLIRFAEA
                     CGTTRERVIDPDNMSPTTRGLQSWCYALAMREDPIVAVAGLVVGLESQVPAIYRNQSP
                     ALREKYGFTDEEVEFFDLHISSDEIHGERGYQIVLELANTPELQQRCVKICEIGAQMR
                     LLYTTALYYDYVAPREATPLAA"
     gene            3935..>5095
                     /locus_tag="VM406_08020"
     CDS             3935..>5095
                     /locus_tag="VM406_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVL75949.1"
                     /translation="MQEPTEYRNLIDGEWARSAAGATFENRNPADTSDLVGLFQASSG
                     DDARRAVDAAARAFAGWKSMPITRRAKILNGAADYLEARADVFAQELTREEGKPLAQA
                     RDEVLRSAQTLRFYAVEGQSFTGETFPNDDPDMLVYTQREPLGVVTVISPWNFPISIP
                     ARKIAPALITGNTVVFKPSSDAPLSGLRLVEALVEAGLPKGVLNCITGRAADVGAAIT
                     EAPQVRAISFTGSTAAGTQIHRSVPLTTRIQMELGGKNPLIVMEDADLDKAVDLAIKG
                     GLSLSGQACTGTSRIIVVAQVKAAFTEKLVEKVRALKIGSGMTGGMDLGPLATQKQLE
                     TVLNYIEIGRQEARLLCGGERLTGEGYERGYYVTPAVFAEVTQQMRIAREEIF"
BASE COUNT          847 a         1671 c         1756 g          821 t
ORIGIN      
        1 gctggcgacg tccacttcgg cctcgcagcg gctttgcaac agcgcgatcg tctccggttt
       61 cgcatagggg tcgcagaccg cgatccggcc taggccgaat gcgcgcgaaa actgttcggc
      121 atgcgcgctg ccctgcacgc cggccccgaa caaggccatg cgctgcggcc tttccggcgc
      181 cagttcgcgc gccgcgagca gcgagcaggc ggcggtgcgg atgcgggtga tcgtgccggc
      241 gtccaggcag gcgagcgggc ggccgtcctc ggtggagaac agcaggatca cgaagcggaa
      301 ctggccgttg atggtggtgt acaccttggc gcctgtcacg ccctggccgg gaatgaccgc
      361 gcccagggtc gacagcttca cgcccccggc ctcggtgcgt gcacgcgcct gcatcgccgc
      421 ccggccgccg gcaaaatcga tgaaggcgct gcgcagcacg gattccgcct cgcttgcact
      481 tacgagtcgt tcgatcatgt cgtcggtcag atgcagcatc gcgcggctcc tttatggcaa
      541 caaacgagtg gggtgacagc agctacttcg tcttctcgcg cgccgtgcgc cggctcttca
      601 tgcgcgcttc ggcggaattg tggacgtgct gcgcggccag cagagccgcg agttccgcgt
      661 tcccggaaat gacggcatgc agcacctgtg cgtgttcttc ccagtcctgg cgtgcgcgtt
      721 ccttcgtgtt cggcgcaaac agcatcgccg tgcgcgtgcg cagcgagcgc accatgtcgg
      781 tcagcaccgc attgccggcc gctgtggcca gcagttcgtg gaattcgtag ttgagcgccg
      841 ccagttgatc cgggcggccg gtcttcgcgg cggccatgcc gcgctccagc acttctttca
      901 gttccgcaac gaccttgtcg gtgcgacgct gtgcggccag cttggcgttc aggccttcca
      961 gcgtcgcgcg cacttcaacg agatcgtgcg ccatctcgtc ggaaagaaac accaccgatg
     1021 cgccgcgccg cggctcgatg gtgaccaggc cctcggacgc gagcgcgcgc agggcctcgc
     1081 gcaccggcac tcgtgaagca ccaagctcct cggacaggcg cccctcgacg aggcggtctc
     1141 cggtggagaa ctcgccgctc aggatgcgtg cgcgcagcgt gtcgcggatg acggcggaaa
     1201 gcggacgatg ttgttcgccg agcttgacat cggcgcgttc ggcgacaacg gccgagggtg
     1261 aagaagtcga catgattctc aggggataaa tggacgtcca gaacattgta tacggccgga
     1321 tcgggaatgg cgagcagggg tgcaaccctt cttctcttgg acaagcggtt gacgaaaagg
     1381 tgtttgctgt atacggtata cagtaagatc ccagccgatt tgctacagca tggacgaccc
     1441 tgaccgccgc tttgccatcg gaagcgtctc gatgatcgcc accggccatc cgctcgcggc
     1501 ggccgccgcg cggcacgcgc ttgctgaagg cgggagcgcg gtcgatgcgg cggtcgcggc
     1561 agatgcggtg ctcggcgtcg tcgagccgat ggcgacaggc ataggcggcg atctgctggc
     1621 catgatcgtc gagccagacg gacaagcggt cgcatacaac gggaccggcc gtgcgcccgc
     1681 atccttgtcg gccgagcgcg tcgcacagat gccaggcggg cgcataccgg aacggcaccc
     1741 atggtcagtc acggtgccgg gcgccgtgtg tggatggcac gacttgcacc agcgctacgg
     1801 ccggctcgaa tggccgcgcc tgttgcgcga tgcgattacg ctggcccgca caggcttcgc
     1861 ggtcgccccg gtggcggcgc gcgagtggaa gctgttcgag ccggtgttga aggcggaccc
     1921 aaccgccgcc gcactgtatc gacacgggcg cgttccggcg gcaggggagg tgtttgccaa
     1981 cccggaactg gcggaattcc ttgcccgcat cgcgcgcgac ggctaccgcg cgttctacga
     2041 tggcagtgcg gccgaggcca tggcggctgc ggtgcgcgcc cgcggtggcc tgctcggcgc
     2101 ggatgacctt gccgcgcatg cgggcaactt ctgcgtgccg ctgcgcggcg agttctgcgg
     2161 cgtcaccgta ctcgaatgcc cgcccaacac gcatggctgc gcggtcatcg acgcgctgct
     2221 gtcgctcgaa gcctttgcgc gcgaagaggg cacgccgcag gcggtcgccg cgctggtcga
     2281 agccaccggg cgcgccttgc agacggccag caagacggtc gccgaccctt ccggcaatac
     2341 ggtgtgcacc gtgatcgtcg accgcgacgg cctcgcgatc acgctgatga gcagcatctt
     2401 caagcgcttc ggttccgcca ttgcggtccc cggccttggc ttcgtgctgc agaaccgtgg
     2461 attcgggttt tccgcgcccg gccatccgaa cgggccggcc ggcggcaagc gcccgtatca
     2521 cacggtgata ccggccgcct gcctgcgcga agggcggttt catgccggtc tcggcgtggt
     2581 tggcggcctg atgcagccgc aagggcaggt gcagctgttg acgcggctct ttgcctgggg
     2641 cgagccgctg cgcgaggcga ttgcggcgcc gcgctggcgg ctggagggcg gccgggcgct
     2701 ggcgctcgag accggcttcc ccgctgatgc ggcagcgctg ttgcgtgagc gcggcttcgg
     2761 tgaacccgct gcaggcgagg gcgatctggc agggcgcagc gacttcggcg gtgcgcaagt
     2821 cgtgctgcgc ctgcccgatg gctcgctcgc aggcgcttcg gacccgcgca aagacggggt
     2881 ggcgctcgcg gactgagagc ttccaccggt aagccgcgtg cgcccgcgcc tgctgcctta
     2941 ctgcaccgca gtgcacgcga acgcccgctt gagcgctcgc aaagtgctgg acaaacgggg
     3001 attttggtat acaactgcat gaagccacgg tatacaatat accgcagcga accgccggca
     3061 gctgttcaca agcgtgccgg cacctaccca aaggagaagc tatgaccgcg ttgatgaatc
     3121 gggaagaatt ccgcgccgct ctggagcgcg cgatccaggg caagaccgca accaaatcgc
     3181 cattcagcgt ggcctgggcc aatggcacgc tgtcgcgcgc ccacctcgcg cactgggcgg
     3241 agaaccacta ccactatgtc ggcccgttcg ccgattacct tgcttacgtt tatgcacgca
     3301 tgccggattc gtataccgaa gcgaaggact tcctgctcta caacatgtac gaggaggaag
     3361 tgggcggaga ccgtcacacc gacctgctga tccgtttcgc cgaagcctgc ggcacgacgc
     3421 gcgagcgggt catcgatccg gacaacatgt cgccgacgac gcgcggactg cagagctggt
     3481 gctacgcgct ggcgatgcgc gaggacccga tcgtcgcggt cgcgggactg gtcgtcgggc
     3541 tcgagtcgca ggtaccggcg atctatcgca accagtcgcc cgcgctgcgc gagaaatacg
     3601 gctttaccga cgaggaggtc gagttcttcg acctgcacat cagctcagac gagatccacg
     3661 gcgagcgcgg ctaccagatc gtgctcgaac tggcgaacac gccggagctg cagcagcgtt
     3721 gcgtgaagat ctgcgagatc ggcgcgcaga tgcgcctgct ctataccacc gcgctgtatt
     3781 acgactatgt ggcgccgcgc gaggcaacgc cgcttgccgc ctgagcgcgc cgcgcacaga
     3841 gaggtgcacg tggaggcgca cggagcgcca cgtgcgccat cgcaccgatg ctccggccgc
     3901 atgcgcaagc cggggcgcat accgaggatg atcgatgcaa gagcctaccg agtacaggaa
     3961 tctcattgat ggcgaatggg cgcgcagtgc ggccggcgcg accttcgaaa accgcaatcc
     4021 ggccgacacc tcggacctgg tcggcctgtt ccaggcttcg agcggcgacg acgccaggcg
     4081 cgcggtggat gccgccgccc gggcgtttgc cggctggaag agcatgccga tcacgcggcg
     4141 cgcaaagatc ctgaacggcg cggcggacta cctcgaagcg cgcgcggacg tgttcgcaca
     4201 agaactgacg cgcgaggaag gcaagccgct cgcgcaggcg cgcgacgagg tgctgcgctc
     4261 ggcccagacc ctgcgcttct acgcggtcga aggccagagc ttcaccggcg aaaccttccc
     4321 gaacgacgat ccggacatgc tcgtctacac gcagcgcgag ccgctgggcg tcgtgacggt
     4381 gatctcgccg tggaacttcc cgatctcgat cccggcgcgc aagatcgcgc cggcactgat
     4441 caccggcaat accgtggtgt tcaagccctc gtcggacgcg ccgctgtccg ggctgcgcct
     4501 ggtcgaggcg cttgtcgagg cgggtttgcc gaagggcgtg ctgaactgca tcaccggccg
     4561 cgccgccgat gtcggcgccg cgatcaccga agcgccgcag gtgcgcgcga tctcgttcac
     4621 cggttccacc gccgccggta cgcagatcca ccgctcggtg ccgctgacca cgcgcatcca
     4681 gatggagctc ggcgggaaga atccgctgat cgtgatggag gatgcggacc tggacaaggc
     4741 ggtcgatctc gcgatcaagg gtgggctatc gctctccggc caggcctgca ccggcaccag
     4801 ccgcatcatc gtcgtcgcgc aggtgaaggc cgcgttcacc gagaaactgg tggagaaggt
     4861 gcgcgcgctg aagatcggca gcggcatgac cggcggcatg gacctcggtc cgctggccac
     4921 gcagaaacag ctggaaacgg tgctgaacta catcgagatc ggccggcagg aggcgcgcct
     4981 gctgtgcggc ggcgaacgcc tgaccggcga agggtatgag cgcggctact atgtcacgcc
     5041 agccgtgttt gccgaggtca cgcagcagat gcgcatcgcg cgcgaggaga tcttc
//
LOCUS       DATLGF010000265        12546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_70409,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000265 DATLGF010000000
VERSION     DATLGF010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 12546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12546
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70409"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1182
                     /locus_tag="VM406_08025"
     CDS             64..1182
                     /locus_tag="VM406_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HVL75950.1"
                     /translation="MDEESTPVPLRPLSPMKGRGAVSNLQGRYELQQREAFDDGWGED
                     SEGASTARRTEVIEETAKSILTRNASPDIPFDVSLNPYRGCEHGCIYCFARPTHSYLG
                     LSPGLDFESRIFAKVNAPELLQQAFARPSYVPEPVALGVNTDAYQPCERELRLTRRIL
                     QIFHDCEHPLALITKSSLIERDIDLLAPMAAKRLAVAAVTITTLDAQIARTLEPRAAA
                     PARRLRTIRTLAEAGIPVGVSIAPVIPFVTEPDLERVLAAALEAGAQSASYIVLRLPW
                     EVNPLFQQWLQAHFPERAQRVMNRLRDMRGGKEYDAQFGTRMRGEGVWADLIRQRFDK
                     ALARLGVRARLGDFAGLDTSHFRAPRQPGRGTAQLDLF"
     gene            1296..3494
                     /locus_tag="VM406_08030"
     CDS             1296..3494
                     /locus_tag="VM406_08030"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HVL75951.1"
                     /translation="MSEEMAVARSRKLMDQLAAHVKQGDHVCVVCEDPQQRLGAAAQY
                     IADGLRQNEFVMYAADAATTATLRTMLSEQGIDVEAEIARGALNLPTAYDAYLSDGEF
                     KPDLMYETFERAIDSALAANFSGCRFAGEPVWAIDRAELRPGLIEFEARLNALFSSRK
                     AAGLCVYDKSAWPAEVVRDIMRIHPVAVVDDAVCKRNVFYERPELVDDSADPESQVAW
                     MLTQLREMRTEEARLEVALEAGQLGSWELDLRNDTSVRSLRHDQIFGYDHLLPEWGYA
                     RFIEHVLPEDRERVSTAFQSAIKHGRTWHFECRIRRHNDGAVRWIEAYGRPDPEGSCG
                     ERVQRLLGIVVDITERKQMEEALRETDRRKDQFLATLAHELRNPLAPILNGLQLMQSK
                     ARFDPELQQLHGMLDRQVRHLARLVDDLLDVSRITTGRIELKRERIELKSVLAGAIEG
                     NQHLIDSLGHAFVVTLPETPIPLDGDGTRLTQVFLNILNNAAKYTPPGGRIELRAERV
                     DGDVIVRVRDNGMGMQAERIPAMFQMFVQGRPSGEQAQGGLGIGLPLARQLLELHGGS
                     IDAQSDGPGCGSEFTVRLPALPAEAPATPQEAKADAITPASAARRILVVDDNVDAADS
                     LAAFLEYLGHQVHTRYDGQSALDELETTGADVVILDIGLPDLDGYTVARRMRALQPDL
                     ILVALTGWGQEQDRRRAEEAGFDLHRTKPVSPAALAADITQIGAVASDGA"
     gene            complement(3498..4283)
                     /locus_tag="VM406_08035"
     CDS             complement(3498..4283)
                     /locus_tag="VM406_08035"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HVL75952.1"
                     /translation="MTSGFRKRFIRIFAFGLIGVASLPLVFVPALRAGALGGAMPDLP
                     LPALVALMLVNPLVLLAIGAALGAALAPRVRLRSFIAMPEVKHEEPIALRSAAPLAVA
                     FGLLLAAATVVLDLALAPLMSEQWSSAVAAQMQQPVLPALVTGMLYGGITEEILLRWG
                     LMSLLMWAGWRLFAGGAAAPPPALAWGAILLAALAFGAGHLPAAAALAPLDAPLVLRT
                     VLLNAIAGVVYGWLFWRRHLEAAMVAHASTHVGFALLRAAGAV"
     gene            4760..5023
                     /gene="infA"
                     /locus_tag="VM406_08040"
     CDS             4760..5023
                     /gene="infA"
                     /locus_tag="VM406_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HVL75953.1"
                     /translation="MAKEDLLEMNGTVAEVLPDSRYRVALENGHQLVAYTSGKMRKNH
                     IRIIAGDRVSLELSPYDLGKGRITFRHLEKRGPVPTGAQRRRR"
     gene            complement(5024..8275)
                     /locus_tag="VM406_08045"
     CDS             complement(5024..8275)
                     /locus_tag="VM406_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HVL75954.1"
                     /translation="MKTRKILIANRGEVAIRVARTAAETGIATVAVYSEDDAQALHVR
                     KAGAARALGGRGPAAYLDAARLIRIAHETGCDALHPGYGFLSENAGFARQCAAAGLLF
                     IGPQPEVLELFGDKARARALAARLGVPLIPGSDGPVTLDEARAFMRTLPAGTPLMLKA
                     VAGGGGRGMRIVTDENDLDEAYTRCAGEARAAFGSDALYAERLVERVRHIEVQVAGDG
                     DDAVHLWERECTLQRRNQKLIEIAPSPSLSPAMRERICADALRLARAVGYRNIGTFEF
                     LVHGDGESAEHWFIEANPRLQVEHTVTEEVLGVDLVKTQIRLAEGASLAQAGLRQALA
                     PRGCAIQLRINMETMQADGGVLSSGGRLQVYTPPSGPGVRVDGCGHAGLAPSPAFDSL
                     LAKLVVSAPGGHADALARARRALDEFEVEGVATNIAFLQALLREPAVQENRVHTRFID
                     EHAATLLAVDHKHASDAAPSPEAATGTMAITAPMHGVLVSLDVAPGAVVRRGQQVAVL
                     EAMKMEHVVHAPFGGTVHATAAAPGEVLAQGQAILHLDHDHSDGDASEAEAGPDLDAI
                     RPDLAEVLARHALLQDEARPDAVARRRKTGQRTARENIADLCDPDSFIEYGALALAAQ
                     RRRRSVEELIRISPADGLVAGIGSVNGAHFGDDRSRCMVISYDYTVFAGTQGMMNHKK
                     TDRMFQVAGQARLPIVLFAEGGGGRPGDTDAMIVAGLDVPTFIGFARLSGLVQRIGIA
                     SGRCFAGNAALLGCCDVIIATENATIGMGGPAMIEGGGLGVFEPEEVGPVSMQAPNGV
                     IDVVVKDEQEAVRVARQYLSYFQGPLPDWRCADQRMLRHLVPENRLRAYDVRRVIDTL
                     ADEDSVLELRREFGVGIITALVRIEGRPLGLIANNPLHLGGAIDADAADKAARFMQLC
                     DAFDLPMLSLCDTPGFMVGPDAEKTAMVRHVSRLFVTAGSMDVPFFTVVLRKGYGLGA
                     QAMAAGSFHAPLLTASWPSGEFGGMGLEGAVRLGYRKELEALTDPAERRALFDRLVGE
                     YYEKGKAISMASFLEIDSVIDPMETRRWIMRGLRSVPPARERGDRKRPFIDTW"
     gene            8365..8586
                     /locus_tag="VM406_08050"
     CDS             8365..8586
                     /locus_tag="VM406_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75955.1"
                     /translation="MHIKVMTRAVPGGDPEPASFAIGTRELQVDEVLDRWPGRLCDYF
                     KIAASDGARYILRHATEEGEWELVWFSAD"
     gene            complement(8866..9075)
                     /locus_tag="VM406_08055"
     CDS             complement(8866..9075)
                     /locus_tag="VM406_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HVL75956.1"
                     /translation="MSTLTGTVKWFNDSKGFGFITPDDGGKDLFAHFSDIQSNGFKSL
                     SENQRVSFVRGMGPKGEKASDIRVV"
     gene            complement(9263..9475)
                     /locus_tag="VM406_08060"
     CDS             complement(9263..9475)
                     /locus_tag="VM406_08060"
                     /inference="COORDINATES: protein motif:HMM:NF021321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HVL75957.1"
                     /translation="MTSATTDPRPTPPERPLPGDCCGSGCARCVYVIYEEEMDAYREA
                     LQEWERRQLSRGKSVDASQSRKARQK"
     gene            9780..10871
                     /locus_tag="VM406_08065"
     CDS             9780..10871
                     /locus_tag="VM406_08065"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HVL75958.1"
                     /translation="MADDAALLRASTGTLNQFVRSTVPGLQYIVVTADRVRFEYAGGW
                     ADIQGHKAMRLDTTLMAYSMTKIFTAVAILQLVEQRRLQLDELIDGHLPHAAYRGHGI
                     TLRQLLTHTSGIPNPVPLRWVHLAEEDPGFDEDEALAAVLRENPVLSFEPGRKFAYSN
                     INYWLLGKIVEQVRGQSFQEYVRLSILRPLQIPPQEMDFVIPDASRHASGYLGKYTWM
                     NLLKGFVLDRKFVGGYEGRWLRLRNHYVNGPAFGGLIGTARGFSRFLQDQLRTESVLL
                     GPQTRRLLETPQTDDAGRAIAMTPGWHVGKTRGVDYLFKEGGGGGFHCEMRIYPTRGI
                     ATVVLANSTDFKSTGLLNRIDHAFLESGQ"
     gene            complement(11257..11967)
                     /locus_tag="VM406_08070"
     CDS             complement(11257..11967)
                     /locus_tag="VM406_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL75959.1"
                     /translation="MSPLPIRQPGAPRILIVDDEPAIVDLLKFTIDKAGWDATLSYSA
                     TEAWELMQRQDFSLVLLDWMLPDLSGYHLLSRIRGNRRTEQLPVIMLTARGMEEDRVA
                     GLDNGADDYVTKPFSPRELTSRINALMRRQGPADPAQLLRAGTVTLDAASVTVTIDNE
                     RAEMGHAEFKLLKFFMSHPERVFSRSQLLDKVWGDHVVIEERTVDVHVLRLRKALKGQ
                     EHLIRTVRGVGYMLTERL"
     gene            complement(11981..>12546)
                     /gene="phoU"
                     /locus_tag="VM406_08075"
     CDS             complement(11981..>12546)
                     /gene="phoU"
                     /locus_tag="VM406_08075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HVL75960.1"
                     /translation="RHLDALRRDLSAVIAAVIARRQPMASDLEEVLADFRIVEDLERV
                     GDLAKNIAKRATAIVNEQFPIDLVLELKRLAGLASKQLEHALNTYLDRDAEKAIAVRD
                     GDEQVDVLHSEVFGKIVARMAEDSGHVLGLVHLLFCAKNIERIGDHATHIAEAAYLTA
                     TGERPASERPRRDLSSTMSADHAASKD"
BASE COUNT         2186 a         4200 c         4082 g         2078 t
ORIGIN      
        1 ttatcgcccc ttgcgacggg tgccgcgcgc atctacacta ctgtttttat atacagtacg
       61 catatggacg aggaatccac cccggtgccg ctgcggccgt tgtcgccgat gaaagggcgc
      121 ggcgcggtat cgaatctgca aggccgctac gaattgcagc agcgcgaggc cttcgatgac
      181 ggctggggcg aagactcgga aggggcgagc acggcacgca ggaccgaggt gatcgaggaa
      241 accgcaaaaa gcatcctcac ccgcaacgcc tcgccggaca ttccgttcga cgtatcgctc
      301 aacccctacc gcggctgcga gcacggctgc atctattgtt tcgcgcgtcc gacccacagc
      361 tacctcggcc tgtcgcccgg actcgacttc gaaagccgca tctttgccaa ggtaaacgcg
      421 cccgaattgc tgcagcaggc ctttgcgcgg ccctcctacg tcccggagcc ggttgcgctg
      481 ggcgtgaaca ccgacgccta ccagccgtgc gaacgcgagc tgcggctcac gcgccggatc
      541 ctgcagatct tccatgactg cgaacatccg ctggcgctga tcaccaaatc ctcgctgatc
      601 gagcgcgaca tcgacctgct cgcgccgatg gccgccaaga ggctggcggt cgctgcggtg
      661 acgatcacca cgctggatgc gcagatcgca cgcacgctgg agccgcgtgc cgccgcgccg
      721 gcacggcgcc tgcgcacgat acgcacgctg gccgaggccg gcattccggt gggcgtgagc
      781 attgcgccgg tgattccgtt cgtcaccgag cccgacctcg aacgggtgct ggccgccgcg
      841 ctggaagccg gcgcacagag cgcaagctat atcgtgctgc gcctgccgtg ggaagtgaac
      901 ccgctgttcc agcagtggct gcaggcgcat tttcccgaac gcgcgcagcg ggtgatgaac
      961 cggctgcgcg acatgcgcgg cggcaaggaa tacgacgcgc agttcggcac gcgcatgcgc
     1021 ggtgaagggg tgtgggcgga cctgatccgc cagcgcttcg acaaggcgct cgcgcgcctg
     1081 ggcgtgcgcg cgcggctggg cgatttcgcc ggactcgaca cctcgcattt ccgagcgccg
     1141 cggcagcccg gccggggcac cgcccagctc gaccttttct agcaaaattt ccaccgggat
     1201 atgacgtaga tcaacctcgt tccacccgca aacacccggc tcccattgag agtaagatac
     1261 gctctctccc ccgcaacccc ctccgctttc tcgtcatgtc tgaagaaatg gctgttgcgc
     1321 gctcgcgcaa gctgatggac cagctggccg cccatgtgaa gcagggcgac cacgtctgcg
     1381 tggtctgcga ggatccgcag cagcggctcg gcgcggcagc ccagtacatc gccgatggat
     1441 tgcgccagaa cgaattcgtc atgtatgcgg ccgatgccgc caccaccgcc accctgcgaa
     1501 cgatgctgag cgagcaaggc atcgacgtgg aagccgaaat cgcgcgcggc gcactgaacc
     1561 tgccgaccgc ctacgacgcg tatctgagcg acggcgaatt caagccggat ctgatgtatg
     1621 agaccttcga gcgcgcgatc gacagcgcgc tcgccgccaa cttttccggc tgccgatttg
     1681 ccggcgagcc ggtctgggcg atcgaccgcg cggaactgcg ccccggcctg atcgagttcg
     1741 aggcgcgcct gaatgcactg ttcagctcgc gcaaggcggc cggtctgtgc gtgtacgaca
     1801 agtccgcctg gccggcggag gtggtgcgcg acatcatgcg catccatccg gtggccgtcg
     1861 tggatgatgc ggtgtgcaag cgcaatgtgt tctacgaacg cccggaactg gtcgacgaca
     1921 gcgccgaccc ggaaagccag gttgcctgga tgctcacgca gctgcgcgag atgcgcaccg
     1981 aagaagcgcg cctggaagtt gcgctggaag ccggccagct cggcagctgg gagctcgacc
     2041 tgcgcaatga cacgtcggtg cgctcgctgc gccacgacca gatcttcggc tacgaccacc
     2101 tgctgcccga atgggggtat gcccgcttca tcgaacacgt actgccggaa gaccgcgagc
     2161 gcgtcagcac ggcgtttcaa tccgcgatca agcacggcag gacctggcac ttcgaatgcc
     2221 ggatccgccg ccacaatgac ggcgcggtgc gctggatcga ggcctacggt cggcctgatc
     2281 cggaaggcag ctgcggcgag cgggtacagc gtctgctcgg catcgtggtg gacatcaccg
     2341 aacgcaagca gatggaagag gcgctgcgcg agaccgacag gcgcaaggac cagttcctcg
     2401 cgaccctcgc gcacgagctg cgcaatccgc tcgcgccgat actcaacggc ctgcagctga
     2461 tgcagtcgaa ggcccggttc gatcccgagc tgcagcagtt gcacggcatg ctggatcgcc
     2521 aggtgcgcca tcttgcacgg ctggtcgatg acctgctgga cgtttcgcgc atcacgaccg
     2581 ggcgcatcga actcaagcgc gagcgcatcg aactcaagag cgtgctggcc ggcgcgatcg
     2641 agggaaacca gcacctgatc gacagcctcg gtcatgcgtt tgtcgtgacc ttgcccgaaa
     2701 cgccgatccc gctcgatggc gacggcacgc gcctgaccca ggtcttcctc aacatcctca
     2761 acaacgccgc caagtacacg ccgccgggcg gacgcattga attgcgggcg gagcgggtgg
     2821 acggcgacgt catcgtgcgg gtgcgcgaca acggcatggg gatgcaggcc gagcgcattc
     2881 ccgcgatgtt ccagatgttc gtgcaaggtc ggcccagcgg cgagcaggcc caggggggac
     2941 tgggcatcgg cctgccgctc gcgcggcaat tgctcgaact gcatggcggc agcattgacg
     3001 cgcaaagcga cggccccggc tgcggcagcg agttcacggt gcgccttccg gccttgccgg
     3061 ccgaggcgcc ggcgacgccg caggaggcga aggccgatgc aatcaccccg gcttcggcag
     3121 cacggcgcat cctcgtggtc gacgacaatg tggacgcagc cgacagcctg gccgccttcc
     3181 ttgagtatct cggccaccag gtgcacaccc gctacgacgg acaaagcgcg cttgacgagc
     3241 tggaaacgac cggagcggac gtcgtgatcc tcgacatcgg cctgccggac ctggacggct
     3301 acacggtcgc ccgccgcatg cgcgcgctgc aaccggacct gattctggtg gcgctgaccg
     3361 gctggggcca ggagcaggac cggcgccggg ccgaggaagc gggcttcgac ctgcaccgca
     3421 cgaagccggt gagcccggcc gcgctcgcgg ccgatatcac gcagatcggc gcggtcgcca
     3481 gcgacggcgc ctgacgctca gaccgccccc gccgcccgca gcagcgcgaa gcccacgtgg
     3541 gtgctcgcgt gcgcgaccat cgcagcctcc aggtgacggc gccagaacag ccagccatac
     3601 acgacgccgg caattgcgtt cagcagcacc gtgcgcagaa cgagcggcgc gtcgagcggc
     3661 gccagcgccg cagccgcggg caggtgaccg gcgccgaagg ccagcgcggc cagcaggatc
     3721 gccccccatg cgagcgccgg cggcggcgcg gccgcgccgc ccgcgaacag gcgccatccg
     3781 gcccacatca gcaaggacat cagcccccac cgcagcagga tctcctcggt gatgccgccg
     3841 tagagcatgc ccgtgaccag cgccggcagc accggctgct gcatctgggc cgcgaccgcg
     3901 ctcgaccatt gctcgctcat caggggcgca agcgcgaggt cgagcacgac ggtcgcggcc
     3961 gccagcagca gcccgaacgc gaccgcaagc ggcgcggcgc tgcgcagggc gatgggttcc
     4021 tcgtgcttga cttccggcat cgcaatgaag gagcgcaggc gcacgcgcgg tgccagtgcc
     4081 gcgcccagcg ccgcgccgat ggccagcaac acgagggggt tgaccagcat gagcgcaacc
     4141 agcgccggca acggcaggtc cggcattgcg ccgcccagcg cgccggcgcg cagcgcaggc
     4201 acgaagacga gcggaaggct ggccacgccg atcagcccga atgcgaagat gcggatgaaa
     4261 cgcttgcgga agcctgaagt catctgtgtt tgagccatgg gaacaaatcc ggggacgagg
     4321 tctctatatg aaggcggccg ttgccgccgc gcagctcacg tgggttcgac agtgcgtgtc
     4381 gaactgacca tgaacgggcc atcagcgccg gcgccattgt gctgccccaa gagggcagtg
     4441 cgcttgatgc gttacaatgt cgcctttacg ccggctgttt ccccctccac cgccggcgcg
     4501 cgtggcaacc tccgctgctg cgcatccgtc gtcaggcgcg tcaccggacc ttgtcgttgc
     4561 gcctgacttg ttccatgcat gccgtcgtcg tttgcggcat cccatctgct catgcacccc
     4621 cgcgtggccc gcatgagcga ctgttcgcat cgccatgcgc cccgcttcga ccacaacggt
     4681 cggcgcacgc atgatcctgt caacccctgt cgcgtgccgc gcgttgccgg catgtcggtg
     4741 ttttattgtc tggagtctta tggccaagga agatctgctc gaaatgaatg gcacggttgc
     4801 cgaagtcctg cccgattcgc gttatcgcgt cgcgctggaa aacggccacc aactggttgc
     4861 ctacacctcc gggaagatgc gcaagaatca catccgcatc atcgccgggg accgcgtatc
     4921 gctggaattg tcgccctatg atctcggcaa gggccgcatc accttccgcc acctcgaaaa
     4981 gcgcgggccg gtgcctaccg gcgcccaacg tcgccgccgt taattaccag gtatcgataa
     5041 acggccgctt cctgtcgccg cgctcgcgcg ccggcggcac cgagcgcagg ccgcgcatga
     5101 tccagcggcg cgtttccatc ggatcgatca cactgtcgat ctcgagaaac gacgccatgc
     5161 tgatcgcctt gcctttctca tagtattcgc cgaccagccg gtcgaacaag gcgcggcgtt
     5221 cggccggatc ggtcagcgct tccagttcct tccgatagcc caggcgcacc gcgccttcca
     5281 gccccatccc gccgaactcg ccgctaggcc atgacgcggt cagcagcggc gcatgaaagc
     5341 tgcccgcggc catcgcctgc gcgcccagtc catagccctt gcgcagcacc accgtgaaaa
     5401 acggaacgtc catgctgccc gcggtgacga acaggcgcga cacatgccgc accatcgcgg
     5461 tcttctccgc atccggcccg accatgaagc cgggcgtgtc gcacagcgag agcatcggca
     5521 gatcgaatgc gtcgcaaagc tgcatgaagc gcgcggcctt gtcggcggca tcggcgtcga
     5581 ttgcgccgcc caggtgcagt ggattgttgg cgatcagtcc gagcgggcgt ccttcgatgc
     5641 gcaccagcgc ggtgatgatg ccgacaccga actcgcgccg cagctccagc accgaatcct
     5701 cgtcggccaa ggtgtcgatc acgcgccgca cgtcgtaggc gcgcagacgg ttctccggca
     5761 ccaggtggcg cagcatgcgc tggtcggcgc agcgccagtc cggcagcggc ccctggaagt
     5821 aggacaggta ttgcctggcc acgcgcaccg cttcctgctc gtccttcacc accacgtcga
     5881 tcaccccgtt gggcgcctgc atggacaccg gccccacttc ctccggctcg aacacaccga
     5941 ggccgccgcc ctcgatcatc gccggaccgc ccatgccgat ggtcgcgttc tcggtggcga
     6001 tgatcacatc gcagcatcca agcagcgccg cattgccggc aaagcagcgg ccggaggcga
     6061 tgccgatgcg ctgcaccagt cccgacaggc gggcaaagcc gatgaaggtg ggcacgtcga
     6121 ggccggccac gatcatcgcg tcggtgtccc cgggacgccc gccgccgccc tcggcgaaca
     6181 gcacgatggg caggcgcgcc tgccccgcca cctggaacat gcggtcggtc ttcttgtggt
     6241 tcatcatccc ctgcgtgccg gcgaacaccg tgtagtcgta ggagatgacc atgcaacgcg
     6301 acctgtcgtc gccgaagtgc gcgccgttga cgctgcctat gcccgcaacc agcccgtctg
     6361 ccgggctgat tcggatcagc tcctccacgc tgcgccggcg ccgctgcgcg gcgagcgcga
     6421 gcgcgccgta ttcgatgaag gagtccggat cgcagaggtc ggcgatgttc tcgcgcgcgg
     6481 tgcgctggcc ggtcttgcgg cgccgcgcga ccgcatcggg acgggcttcg tcctgcagca
     6541 atgcatggcg cgcgagcact tcggcaagat ccgggcggat cgcatcgaga tcgggcccgg
     6601 cctcggcttc gctcgcgtcg ccatcgctgt gatcatgatc gagatgcagg atcgcctgcc
     6661 cctgcgcgag cacctcgccc ggcgcggccg ccgtcgcatg cacggtcccg ccgaacggcg
     6721 catgcacgac atgctccatc ttcatcgctt cgagcaccgc cacctgctgt ccgcgccgca
     6781 ccaccgcgcc gggcgcgaca tccagtgaaa ccagcacgcc gtgcatcggt gccgtaatgg
     6841 ccatggtgcc ggtcgcggct tcgggcgatg gcgcagcgtc ggaggcgtgc ttgtggtcaa
     6901 ctgcaagcag tgtcgcggcg tgctcgtcga tgaaacgcgt gtgcacccgg ttttcctgca
     6961 ccgccggttc gcgcagcagc gcctgcagga acgcgatgtt ggtcgccacg ccttccacct
     7021 cgaactcgtc cagcgcacga cgggcgcgcg cgagcgcatc ggcatggccg cccggcgcgc
     7081 tgaccaccag tttcgccagc agcgaatcga atgcaggact cggtgcgagg ccggcatgtc
     7141 cgcagccgtc cacacgcacg cccggtccgc tgggcggcgt gtacacctgc aggcgtccgc
     7201 ccgacgacag cacgccgccg tcggcctgca tggtctccat gttgatgcgc agctggatcg
     7261 cgcagccgcg cggtgcgagc gcctgtcgca gcccggcctg cgcgagcgac gcgccttcgg
     7321 ctagccggat ctgcgtcttc acgagatcca cgccaagcac ttcctcggtc accgtgtgct
     7381 cgacctgcag gcgcggattg gcctcgatga accaatgctc ggccgactca ccgtcaccgt
     7441 gcaccaggaa ttcgaaggtg ccgatgttgc gatagccgac cgcgcgcgcg agccgcagcg
     7501 cgtcggcgca gatgcgctcg cgcatcgcgg gcgacagcga agggctggga gcgatctcga
     7561 tcagcttctg gttgcgccgc tgcagcgtgc attcgcgctc ccacagatgc accgcgtcat
     7621 cgccgtcgcc ggccacctgc acttcgatat gacgcacgcg ttcgacaaga cgctcggcat
     7681 acagcgcgtc gctgccgaat gccgcgcgcg cctcgccggc gcagcgcgtg tatgcctcgt
     7741 caagatcgtt ctcgtcggtg acgatgcgca tcccgcgtcc gccgccgccc gccaccgcct
     7801 tgagcatcag cggcgtcccg gccggcagcg tgcgcatgaa ggcgcgtgcc tcgtccagcg
     7861 tgaccgggcc gtcgctgccc gggatcagcg gcacgccgag ccgtgcggcc agcgcgcggg
     7921 cgcgcgcctt gtcgccgaac agttccagca cctccggctg cggcccgatg aacagcaggc
     7981 cggccgcggc gcattgccgt gcgaaaccgg cgttttcgct gagaaatccg tagcccgggt
     8041 gcagcgcatc gcagccggtc tcgtgcgcga tgcggatcag ccgggcggcg tcgagatagg
     8101 cggcggggcc gcgtccgccc agcgcgcgcg ccgcgccggc cttgcgcacg tgcagcgcct
     8161 gcgcatcatc ctcggaatac accgccaccg tcgcaatgcc ggtctcagcg gcggtgcggg
     8221 caacgcggat cgccacctcg ccgcgattgg cgatcaggat ctttcttgtc ttcatgtctc
     8281 ctccttcggc cagacattct gcctgcatcg ctgtgcgttc gtcgaatgcg aaccttggcg
     8341 catggaccga cacaaacagg gatcatgcac atcaaggtaa tgacacgcgc ggttcccggc
     8401 ggcgatccgg aaccggcttc gttcgcgatc ggaacgcgtg aattgcaggt cgacgaggta
     8461 ctggatcggt ggccgggccg gctctgcgac tatttcaaga ttgcggcaag cgatggcgcg
     8521 cgctatatcc tgcgccatgc gaccgaagaa ggagagtggg agcttgtctg gttcagtgct
     8581 gactgagcgg cctgcccggg tgtgggacaa cgaaccaccc gtgtagctgg acaacatgaa
     8641 aggcccgctg cggagggctg aagggttacg aggagctgaa acgcaaaaag gctcgccaca
     8701 tggcgagcct tttctattca tcacaccagc cggattgtca gacgcccatg ctatctgcaa
     8761 ggtgcccctg attcctcgcg ggtacccatg ctatctgcat ggtgccccgg ggaggcgccg
     8821 gtggcgctcg gtgaattctg gagatcgtgg ggctagaggc cggactcaga caacacggat
     8881 gtcgcttgcc ttttcgccct tcgggcccat gccgcgcacg aaggagacgc gctggttttc
     8941 cgacaagctc ttgaagccgt tcgattggat atcgctgaag tgggcaaaca ggtccttgcc
     9001 gccgtcatcg ggagtgatga agccgaagcc cttggagtcg ttgaaccact taacggtacc
     9061 agtgagtgta ctcatgaaaa attcctgaaa aagatgtgtg aagggcacga tgcccaaagt
     9121 tggcacccga aaccaagatg taaccgaagg gagtcaaaca ggaattgctc gatgagcggg
     9181 caagacggga aagagaccaa caataaccac ttgatgtaag tgcagggccg actatacaca
     9241 cataagcgga gatcaccaag gtttattttt ggcgcgcctt gcgagattgg cttgcgtcaa
     9301 cgctctttcc tctggacagc tggcgtcgct cccattcctg caacgcctcc cggtacgcgt
     9361 ccatctcctc ttcgtagatc acgtacacgc agcgcgcgca gccgctgccg cagcagtcgc
     9421 cgggcagcgg gcgttcggga ggagtgggac ggggatcggt cgttgcgctc gtcatccgcc
     9481 aaggatagcg gagaccgtgc agagacggcg gaagggcaac atggccatcc gtgccgggcg
     9541 caggcgttcg gaaagcactt cgacagattc acgcgcgcgt tccggaagac ggcggaattc
     9601 ttggcgagag cacggcgatc ctgatgaacc tcgcagatac gcgtcccaca tcccggcgac
     9661 caggcgaatc gattcagggc cgcccgaagt ctcaggggaa cgccgcttcc cgtcccggca
     9721 ggtgtacggt aaagctgccg gatgcccgcg gcatcttgca ggaggatgca ggaggaacga
     9781 tggccgacga cgctgccttg ttgcgcgcct cgacaggtac gctgaatcaa ttcgttcggt
     9841 cgacggttcc cggccttcag tacatcgtcg tgaccgccga tcgtgtgcgc ttcgaatatg
     9901 caggcggctg ggccgatatc cagggtcaca aggcaatgcg cctggacacg accttgatgg
     9961 cgtactcgat gacgaagatc ttcaccgctg tcgcgatcct gcagttggtg gagcagcgca
    10021 ggctgcaact cgatgagctg atcgacggcc accttccaca tgccgcctac cgcggccatg
    10081 gcatcacctt gcgccagctg ctgactcaca cgtccggcat tccaaacccg gttccgcttc
    10141 gatgggtgca cctggccgaa gaggacccgg ggttcgatga ggatgaggcg cttgctgcgg
    10201 ttctgcgcga gaaccctgta ctttcgtttg aaccgggccg caagtttgcc tactccaata
    10261 tcaattactg gttgctcgga aagatcgtcg agcaggtgcg ggggcagtca tttcaggagt
    10321 atgtgcgact gagcatcctt cggcccctgc agatcccccc gcaggagatg gactttgtca
    10381 ttcccgatgc gtcgcgccat gcgagcggct atcttggaaa atacacgtgg atgaacctgt
    10441 tgaagggctt cgttcttgat cgcaagttcg tgggcggata cgagggacgg tggctgcggc
    10501 tgcgcaacca ttacgtgaac ggccccgctt ttggcgggct gatcggcacg gcgcgcgggt
    10561 tcagccgatt cctgcaagac cagttgcgaa ccgagtccgt actgctggga ccgcagacca
    10621 ggcgccttct ggagaccccg cagaccgacg atgccgggcg ggcaatcgcc atgacgcccg
    10681 gatggcatgt cggcaagacc cgcggggtgg actatctctt caaggaaggg ggcggcggcg
    10741 gatttcattg cgagatgcgc atctacccga caagaggcat tgctacggta gtcctggcaa
    10801 acagtacgga tttcaagtcc accggattgt tgaatcgcat cgaccatgca ttcctggaat
    10861 ccggccagtg aaaatgcggc ccgcgatttg ccggatgccc gttggagtgt catgcaccgg
    10921 gatgactgcc ccgcatgacc atctccgccg caaccaccag attgcgcccg tgcgtacgac
    10981 cgggctggcg aggatgcgcg tgtcttcacg caggtgacgc cgcagccaca cggccatcac
    11041 gcgcgcatga tcgccgagct cgcgcaggcg caccatgttg accgggccgt cgactgcata
    11101 taaccggctt ctgtcgatgc cgaactgttc gaggaggaaa cctcgcggtc gaactggtcc
    11161 tgcgggtgcg cgtggatgac ggaagacagc aggcagaagc agatgccgtt cttgcgatcc
    11221 gtgccacaac cgcgccgccc gcgcgcccgt ccggtttcat agccgttccg tcagcatgta
    11281 gccgacgccg cgcacggtcc ggatcagatg ctcctgcccc ttgagcgcct tgcgcaggcg
    11341 tagcacgtgc acgtcgaccg tgcgttcctc gatgaccaca tggtcgcccc agaccttgtc
    11401 gagcaactgg ctgcgcgaaa acacgcgctc ggggtggctc atgaagaact tgagcagctt
    11461 gaactcggca tggcccatct cggcgcgctc gttgtcgatc gtgaccgtta cgctggccgc
    11521 atccagcgtg accgtgcccg cgcgcagcag ctgggccgga tcggccggcc cctggcggcg
    11581 catcagcgcg ttaatgcggg atgtcagctc gcgcggcgag aacggcttgg tcacataatc
    11641 gtcggcgccg ttgtcgaggc ccgcgacgcg gtcttcctcc atgccgcgcg cggtcagcat
    11701 gatgaccggc aattgctcgg tgcgccggtt gccgcggatg cgcgagagca ggtggtagcc
    11761 ggacaggtcc ggcagcatcc agtcgagcag caccaggctg aaatcctggc gctgcatcag
    11821 ctcccaggcc tcggtcgcgc tgtagctgag ggtcgcgtcc cagcccgcct tgtctatcgt
    11881 gaacttgagc aggtccacga tcgccggttc gtcatcgacg atcagtatgc gcggcgcgcc
    11941 gggctgcctg atcgggagcg gtgacatcgt ggtcgtgtag tcagtccttg cttgcagcat
    12001 ggtcggcgct catcgtgctg gagagatcgc ggcgcgggcg ttcggaggcc gggcgctcgc
    12061 cggttgcggt caggtaggcg gcctcggcga tatgggtcgc atggtcgccg atgcgttcga
    12121 tgttctttgc gcagaacagc aggtgcacga ggccgagcac atggccgctg tcctcggcca
    12181 tgcgggcgac gatcttgccg aacacttcgc tgtgcagcac gtcgacctgt tcgtcgccgt
    12241 cgcggaccgc gatcgccttc tcggcatcgc ggtccaggta ggtgttgagc gcgtgctcga
    12301 gctgcttcga agccagcccg gccaggcgct tgagctccag caccagatcg atcggaaact
    12361 gctcgttgac gatggcggtg gcgcgcttgg cgatgttctt cgccaggtcg ccgacacgct
    12421 cgaggtcctc gacgatgcgg aaatcggcga gcacttcttc caggtcgctg gccatcggct
    12481 ggcgccgtgc gatgacggcg gcgatcaccg cggacagatc gcggcgcagc gcgtcgaggt
    12541 ggcgat
//
LOCUS       DATLGF010000266         4100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_195107, whole genome shotgun sequence.
ACCESSION   DATLGF010000266 DATLGF010000000
VERSION     DATLGF010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195107"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VM406_08080"
     CDS             complement(<1..405)
                     /locus_tag="VM406_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HVL75961.1"
                     /translation="MSSISIDLHDLQILDLLQQDGRATNATLGEKIHLSSSQVSRRVQ
                     RLEEAQVISRYAALINPEIAGLGVEAYTFVTLGRHGESHGDTFERRVVDMPEVLDCIA
                     VSGECDYILRVVAPDLAAFSEFMMKQLLRLPGV"
     gene            578..1654
                     /gene="hppD"
                     /locus_tag="VM406_08085"
     CDS             578..1654
                     /gene="hppD"
                     /locus_tag="VM406_08085"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HVL75962.1"
                     /translation="MSDANFNPMGLCGFEFVEFASPVPNVLEPVFEMMGFTKVAVHRS
                     KDVVLYRQGGINFIVNNEPNSHAAYFAKEHGPSACGMAFRVKDSHKAYARALELGAQP
                     IEIPTGPMELRLPAIKGIGGAPLYLIDRFEEGKSIYDIDFEFIEGVDRNPKGTGLKII
                     DHLTHNVYRGRMAFWANFYESLFNFREIRYFDIKGEYTGLTSKAMTAPDGMIRIPLNE
                     ESSKGSGQIEEFLMQFNGEGIQHIALLTDDLKSTLDQLALNGVPFMPSPPATYYEMLQ
                     ERLPGHGEAVEELKTRGILLDGSTEGGQKRLLLQIFSKNLLGPVFFEFIQRKGDEGFG
                     EGNFKALFESIERDQIQRGVLQTA"
     gene            2150..2608
                     /locus_tag="VM406_08090"
     CDS             2150..2608
                     /locus_tag="VM406_08090"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HVL75963.1"
                     /translation="MNRFIRRWGASVLVTGIGVATVFGGMAHHVGTAARMGPGYFPIA
                     LGILLILLGAIIGLVTDRDALEKEPADPRGVDLRGWFCVIAGIVLFMVLGEYAGFIPG
                     TFALVFVAALGDREHSLLSAALLAAGVTVLGVLVFTQLLELNIPLFKLGG"
     gene            2615..>4100
                     /locus_tag="VM406_08095"
     CDS             2615..>4100
                     /locus_tag="VM406_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HVL75964.1"
                     /translation="METLNGITLGFGAALAPGNLMYAFLGVVLGNLVGVLPGIGSLAA
                     ISMLLPITFSIEPAAALMMLAGIYYGAQYGGATTSIMLNLPGVASHAVTCLDGHPMAK
                     NGRAGPALLLAMLASFVGATIGIVIMMFFSPVIAETALKFGPAEYFSMMVLGLLAATT
                     LSTGSVIKGVVMVVLGVVIGIVGTDVNTGTVRFAFGTPYLYEGVSLIALAMGLFGIAD
                     VLRSVNRMGKAQVQGRVGWRSLKLSRDDVARSGKPVVRGAGIGAFFGVLPGTGSTIAS
                     FMSYALEKKVSKTPQAFGKGAVEGVVGPEASNNAAAQTAFIPTMTLGIPGDAVMALML
                     GAMMIHGITPGPTLIGEYPEVFWGLIASFWIGNLLLMVLNVPLIGLWVRLLTVPYRFI
                     YPAVLFFICVGVYSYHNTYFDVALVLLFGMAGFMLMRMGFEPAPLLLGFVLGPMLEEY
                     LRRALLLSRGDPMVLLQRPLSASFLAIGAALVLWTLWSTYRGSAR"
BASE COUNT          673 a         1306 c         1313 g          808 t
ORIGIN      
        1 cacccccggc aggcgcagca actgcttcat catgaactcc gagaaggccg ccaggtccgg
       61 cgccaccacc cgcaggatgt agtcgcattc gccggaaacg gcgatgcagt ccagcacctc
      121 cggcatgtcg accaccctgc gctcgaaggt gtcgccgtgg ctttcgccat ggcgccccag
      181 cgtgacgaag gtgtaggcct cgacgcccag tccggcaatc tccgggttga tcagggccgc
      241 gtagcggctg atgacctgag cctcttccag ccgctgcacg cggcggctga cctgggagct
      301 ggagaggtga atcttctccc ccagcgtcgc gttggtcgcc cggccgtcct gctgcagcag
      361 gtcgaggatt tgcaggtcgt gaagatcgat ggaaatgctg gacatggttc gtgcgcgttc
      421 tgacgtgaag gtgaatgaaa tgtgcgaagt atcgacaatt ctatgtcgat ttcgcgcgca
      481 atatgcgctt cgatcgcatt aacatcattg ccatcgaggt ggcctgccgc cactggtttt
      541 ttgtttgcct tacatcactc ttccgaggaa ctgcgccatg tctgacgcca atttcaaccc
      601 catgggcctg tgcggattcg aattcgtgga attcgcttcg cccgtgccga acgtgctcga
      661 gcccgtcttc gagatgatgg gtttcaccaa ggtcgccgtc caccgatcga aagacgtggt
      721 gctgtatcgc cagggcggca tcaacttcat cgtcaacaac gagccgaaca gccacgccgc
      781 ctacttcgca aaagaacatg gtccgtccgc ctgcggcatg gccttccgcg tcaaggactc
      841 gcacaaggct tatgcgcggg cgctggaact gggcgcgcag ccgatcgaga tcccgaccgg
      901 cccgatggag ttgcgtctgc cggcgatcaa gggcatcggc ggcgcgccgc tgtacctgat
      961 cgaccgtttc gaggaaggca agtccatcta tgacatcgac ttcgagttca tcgaaggcgt
     1021 ggaccgcaac ccgaagggca ccggcctgaa gatcatcgac cacctgaccc ataacgtcta
     1081 tcgcggccgc atggccttct gggccaattt ctacgagagt ctgttcaatt tccgcgagat
     1141 ccgctacttc gacatcaagg gcgaatacac cggcctgacc tcgaaggcga tgaccgcgcc
     1201 ggacggcatg atccgcattc cgctgaatga ggaatcgtcg aagggctccg gccagatcga
     1261 ggaattcctg atgcagttca acggcgaagg catccagcac atcgcgcttc tgaccgacga
     1321 cctgaagtcc acgctggacc agctcgccct gaacggcgtg cccttcatgc cgtcgccgcc
     1381 ggcgacctac tacgagatgc tgcaagagcg tctgccgggc catggcgagg cggtcgagga
     1441 actgaagacg cgcggcatcc tgctcgacgg ttccaccgaa ggcggccaga agcgcctctt
     1501 gctgcagatc ttctcgaaga acctgctggg cccggtgttc ttcgaattca tccagcgcaa
     1561 gggcgacgaa ggcttcggcg aaggcaactt caaggccttg ttcgaatcga tcgaacgcga
     1621 ccagatccag cgcggcgtgc tgcagaccgc gtaatgcgaa gcatggcagg gcgcacacgc
     1681 tgcgccttgc gccttgtctg aatcaacttc ccttctgccc cggcgcgtgt gacgcaacgg
     1741 ggcttttttc gcgcgcatcg aacgccatcg ggcggcgcgc gtcctcaata ccctccagtt
     1801 cctcttctcc gcgcggttgc agaacctgct tgcccaagcg attgcccgcg aggcgggaat
     1861 cccgttcgca tgagcgctga ctccgcacat gtcgcccccg cgaaggcggc agcccagctc
     1921 cggctttcgc aggctgcgaa ccccggaccc gatccccgcc cgaagggaac tcgccgagcg
     1981 acccgttcct tgcatctcgg aaaaatgcaa gccctgaacc gctccctttc ttagacgcaa
     2041 cgcaaatttc ttgcctgaac gccacctctt atttgcggtc aagaaactgt ggcagtgcgt
     2101 atctgtacac aaagtgaaac atccgtaaca aaagaagggg aggaaatcca tgaatcgctt
     2161 catcaggcgc tggggagcat ccgtgctcgt caccggcatc ggcgtcgcca cggtattcgg
     2221 cggcatggcc catcacgtcg gcacggccgc acggatgggg ccggggtact ttccgatcgc
     2281 gctcggcatc ctgctgattc ttctgggggc catcatcggc ctggtgacgg accgcgacgc
     2341 gctggagaag gaacctgccg atccgcgtgg cgtggatctg cgcggctggt tctgcgtgat
     2401 cgccggcatc gtgctgttca tggtgctggg cgagtatgcc ggcttcattc ccggcacctt
     2461 tgcactggtg ttcgtcgccg cgctcggcga tcgtgaacat tcgctgctct ctgcggcctt
     2521 gcttgctgcc ggggtcaccg tgctcggcgt gctggtgttc acccagctgc tcgagctgaa
     2581 catccccctc ttcaagctgg ggggctgagc gatgatggag accttgaacg gcattacgct
     2641 cggctttggc gccgccctgg cgccgggcaa cctgatgtat gcgttcctcg gcgtggtgct
     2701 cggcaacctg gtcggcgtgc tgcccggcat cgggtcgctt gcggcgatct cgatgctgct
     2761 gccgatcact ttctcgatcg agccggccgc cgcgttgatg atgctggccg ggatctacta
     2821 cggcgcccag tacggcggtg cgaccacctc gatcatgctg aacctgcccg gcgtggcctc
     2881 gcatgcggtc acctgcctgg acggccaccc gatggccaag aacgggcgcg ccggaccggc
     2941 gctgctgctg gcgatgctcg cctcgttcgt cggcgccacc atcggcatcg tgatcatgat
     3001 gttcttctcg ccggtgattg ccgagaccgc gctgaagttc ggcccggccg agtacttctc
     3061 gatgatggtg ctgggcctct tggccgcgac cacgctgtcg accggctcgg tgatcaaggg
     3121 cgtggtgatg gtggtgctgg gcgtggtgat cggcatcgtc ggcaccgacg tgaacaccgg
     3181 caccgtgcgc tttgcgttcg gcacgcccta cctgtacgaa ggggtgagcc tgatcgcgct
     3241 ggcgatgggc ctgttcggca tcgccgacgt gctgcgcagc gtcaaccgca tgggcaaggc
     3301 gcaggtgcag ggccgggtcg gctggcgcag cctgaagctc agccgcgacg acgtcgcgcg
     3361 ctccggcaag ccggtggtgc gcggcgccgg catcggcgcg ttcttcggcg tgctgcccgg
     3421 caccggctcg acgatcgcct cgttcatgtc gtatgcgctc gagaagaagg tgtcgaagac
     3481 gccgcaggcg ttcggcaagg gcgcggtcga aggggtggtc gggccggagg cgtcgaacaa
     3541 tgcggccgcg cagaccgcgt tcatcccgac catgacgctg ggcattccgg gcgatgcggt
     3601 gatggcgctg atgctgggcg cgatgatgat ccacgggatc acgccggggc cgacgctgat
     3661 cggcgaatac ccggaagtgt tctggggcct gatcgcgtcg ttctggatcg gcaacctgct
     3721 gctgatggtg ttgaacgtgc cgctgatcgg gctgtgggtg cgcctgctga ctgtgccgta
     3781 ccgcttcatc tacccggcgg tgctgttctt catctgcgtc ggcgtgtact cgtaccacaa
     3841 cacctatttc gacgtggcgc tggtgctgct gttcggcatg gccggcttca tgctgatgcg
     3901 catgggcttc gaaccggcgc cgctgctcct gggcttcgtg ctcgggccga tgctggagga
     3961 atacctgcgc cgcgcgctgc tgctgtcgcg cggcgatccg atggtgctgc tgcagcggcc
     4021 gctgtcggcc agcttcctcg cgatcggcgc ggcgctagtg ctgtggacgc tctggtccac
     4081 gtaccgcggc agcgcgaggt
//
LOCUS       DATLGF010000267        17958 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_118116, whole genome shotgun sequence.
ACCESSION   DATLGF010000267 DATLGF010000000
VERSION     DATLGF010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 17958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17958
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118116"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VM406_08100"
     CDS             <1..706
                     /locus_tag="VM406_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75965.1"
                     /translation="FMISRLKPEGELSGEEARHAWPGGAWKLGRRKCRSFKRFSSLCR
                     RGAGGHRSIRRAPGAHVACATGPDRRENAHQTRKYRPCYDESPHWPHLPLLPRMQELC
                     NEPFLHLCLDPDQRILCAQWKGYQSFDNGVAGCHRILDEATRHGISRILNDNTLVRGL
                     WMDAAEWAGRIWFPRLRQAGVKHFAWVCSPARFSRISMSAALAVIDDTELDLRVFQTV
                     EEASVWLHSVSDASPQ"
     gene            complement(790..1533)
                     /locus_tag="VM406_08105"
     CDS             complement(790..1533)
                     /locus_tag="VM406_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HVL75966.1"
                     /translation="MTEPIISFEKVNKWYGNNFHVLRDIDLQVKRGERIVICGPSGSG
                     KSTLIRCINRLEEHQEGRLVVDGIELADDVKAIDQVRKNVGMVFQQFNLFPHLTVLEN
                     LTLSPMWVGKMPKKDAEARAMQQLERVQIAEQAHKYPLQLSGGQQQRVAIARALCLTP
                     KIMLFDEPTSALDPEMIKEVLDVMIELAGQGITMICVTHEMGFAKAVADRVIFMDQGQ
                     IIEENTPHEFFNNPRNERSKDFLSKILGH"
     gene            complement(1572..2630)
                     /locus_tag="VM406_08110"
     CDS             complement(1572..2630)
                     /locus_tag="VM406_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HVL75967.1"
                     /translation="MRDEGVIGWTRANLFGDWKTSIGTLIVGGLLLWYIPQLISWGLF
                     RAVWAPDSDLCRAEGVGACWGVIAEKYRLIIFGRYPFDEQWRPLVATILMVLLLVASC
                     TRAFWRPWLAGLWVVILAAFYVLMAGGWFGLTPVDTDRWGGLPLTILLATLSIVMAFP
                     IALLVALGRRSELPAIRTVCTIYVELIRGVPLISVLFMASFMFPLFMPEGMTIDVLLR
                     VLIGITLFAAAYMAEVIRGGLQAIPKGQIEAAATLGLSYWQTQRKIVLPQALAMVVPG
                     IMNNFIAIFKDTSLVTIVSLYELTGSMGLALNSDALWRPYKIEGYLFIAAIYFAFCFA
                     MSRYSLWVEKQVNKGRQR"
     gene            complement(2722..3831)
                     /locus_tag="VM406_08115"
     CDS             complement(2722..3831)
                     /locus_tag="VM406_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516872.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVL75968.1"
                     /translation="MYQIVALVLIGLAVWFLAHNTAQNMRIRGIQSGFDFLGQTAGFD
                     IGEFIIPYDSFDPYWNAFLVGLANTLRVAILGIILTTIIGTLIGIGRLSRNALIRSVC
                     GAYVEIFRNIPILLQLLMWYLVFTEFLPAVYEAWEVGPFFLSKSGVNFPIPIWDKDVI
                     WPLAGLAAGIALAWLYRRWSRKRFEETGKPPSIFLIPALIIVAMTVLGWLAGGAETQF
                     EVPGRGDFAVEGGGALTPEFLAVLLGLVIYTAAFVAEVVRAGVQSVSHGQGEAAAALG
                     LSRGQAMRLVMLPQALRVIIPPLTNQYLNLTKNSSLAVAIGYPDVVSIANTAINQTGR
                     AVECIAIIMLVYLTTSLTTSGLMNWYNARAAIKER"
     gene            complement(4124..5155)
                     /locus_tag="VM406_08120"
     CDS             complement(4124..5155)
                     /locus_tag="VM406_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HVL75969.1"
                     /translation="MKLSKIKLATAAVVTLGTLLSLPAHAGRTLDSIKQRGNLVCGVN
                     TGLAGFSQADSNGRWSGLDVDICRAVAAAILNDGEKVRYVPLTAQQRFTALQSGEVDI
                     LSRNTTFTLTRDASLGLHSTAVTYYDGQGFMVPAKTNIKSAKQLKGQTVCVQSGTTTE
                     KNLTDYSRANGLNIKPVVFEKVEAATGAYFAGRCVAYTTDASGLASVRNQEARVPAEH
                     VILPELISKEPLGPMVRRGDDELFAIVKWVIYGLLEAEEFGVTQANVDQMRTSQDPVV
                     QRLLGTSEDTGRLLGLDKDWLGRAIKTTGNYGEIFERNVGPKSPLGLPRGLNNQWNKG
                     GLMYAYPVR"
     gene            complement(5619..5873)
                     /locus_tag="VM406_08125"
     CDS             complement(5619..5873)
                     /locus_tag="VM406_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75970.1"
                     /translation="MEKQAAPVNSPQLSGARPPLPRPGAKPGKARLDRTEISRCINIW
                     ASVYRLREPRAGEDELGGSELQMPPRTWTGRSIPRADRFK"
     gene            complement(6105..7559)
                     /locus_tag="VM406_08130"
     CDS             complement(6105..7559)
                     /locus_tag="VM406_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75971.1"
                     /translation="MQAHPARKFLPLLLVAALSACGGGSSDTPSGNPPFGNPPGDTNP
                     PPGGTNPPPQQATPMTMHCVDGTDFQCSGATVLRVENGVALTDSGVQVYGISTSDLDP
                     GHTVTTRAWGLRPASGGPAEVRVRRNAEGSTSAFYLLLRNLGLSWDGVNERPPIFETF
                     NPTQGRVELDANGRITFVPLPDSSDLAFYDYATLGRAATQARYANNRYFPRENNPPRC
                     PGGPGTCASTETDGVRTAARPPGSWHNGGLTPDFASVQRFHSDGDVHAGDGPPDANGN
                     RTWLPGGTGFGIPFPASKGYRDMTNWSYRYANLATWTTQDTVVIAEWATTTPEHNKLR
                     RGVVSFGDVTPPAEVPTVGSATYTGIAYGWYAGREDVEELPPYWGTARVEVNFATRTA
                     VVTIQDTNTYDGSGSPVPAAFTAALAGSGDAEANYLTGSAAAGMMNGGISARLYGPSA
                     GMGTAAPAEVGGAFMLTHPATGQTIMGGFLGRRQ"
     gene            complement(7990..8205)
                     /locus_tag="VM406_08135"
                     /pseudo
     CDS             complement(7990..8205)
                     /locus_tag="VM406_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394276.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
     gene            complement(8693..9802)
                     /locus_tag="VM406_08140"
     CDS             complement(8693..9802)
                     /locus_tag="VM406_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HVL75972.1"
                     /translation="MTERMPDWLLAIVYLITPWIVLIRGLPWISVLVQVLIALPVALL
                     VHAGATAMLRRLAAPLPYSRQVVDYAHGAGGLLFFLLLVQIILRNAPPHLTGLELARH
                     LNAVAMVITLTWLGVCVVRGIAHAIVELHPADAIDNFHARRIQTQTKVLARSVMVLIV
                     LIGTASALMTLPLLRQVGTSLLASAGVAGLVLGFAAKPVLGNLLAGLQIALTQPIRLD
                     DVVIINNEWGQIEEITGTYVVVRIWDQRRLVVPLQWFIENPFQNWTRRTSDILGTVFL
                     WVDYRMPLDVLRTQAQAVCTAAPEWDGRLCLIQVVDTNERAMQLRVLVSAADAGKAWD
                     LRCRLREGLIDFVQRSYPQYLPRLRAEFTATPGPT"
     gene            complement(9820..9984)
                     /locus_tag="VM406_08145"
     CDS             complement(9820..9984)
                     /locus_tag="VM406_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75973.1"
                     /translation="MSPAEAHQVPPDPVPGDDPMPDKNPSPDDEPVPDHHPVLYPPPA
                     LPEESLVSAA"
     gene            complement(10354..10671)
                     /locus_tag="VM406_08150"
     CDS             complement(10354..10671)
                     /locus_tag="VM406_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75974.1"
                     /translation="MNETKETSAAEQQQQFQYDPNNLLESLIERLNLKNDAALSRALE
                     VAPPLISKIRHRRLPVGASLLIRMHEVSQLSIKDLRELMGDRRAKFRISDKQFKPKQP
                     NAS"
     gene            complement(10792..11046)
                     /locus_tag="VM406_08155"
     CDS             complement(10792..11046)
                     /locus_tag="VM406_08155"
                     /inference="COORDINATES: protein motif:HMM:NF019016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 family protein"
                     /protein_id="HVL75975.1"
                     /translation="MPRTTTEPRVGEEGVTFMVEVGFADRECVVSKNALAHLARLKGG
                     SLDYLQTYRAFEDQIHSVARRLVVAGVSGSPLVLGAAYFV"
     gene            11355..11582
                     /locus_tag="VM406_08160"
     CDS             11355..11582
                     /locus_tag="VM406_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75976.1"
                     /translation="MTPEEIVADLNARNREALDARGHAGSFTSEQVLALMDKAAMHGF
                     KVGSNVALSMVKGSLLVQLNRVAARQDRRRP"
     gene            11688..12206
                     /locus_tag="VM406_08165"
     CDS             11688..12206
                     /locus_tag="VM406_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HVL75977.1"
                     /translation="MSLHHKRSQILAVMLLTGAVPFASANGFDDFARVVSVTPQYEQV
                     NHPRQECVTEYVQVQRPAQDRGMAGAVIGGIAGGLLGNQVGGGSGRTAATAAGAIAGT
                     IVGDRIENSNQPGTVVEQQPVQRCRTVDNWQTRANGFSVTYEYRGFMHTTTLPNHPGE
                     RLPVRVSVTPRM"
     gene            12510..14723
                     /locus_tag="VM406_08170"
     CDS             12510..14723
                     /locus_tag="VM406_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HVL75978.1"
                     /translation="MDKQLKPVPDSHQPDRRQPLELWGGMECTVARLGDRFRDQLRET
                     GHAQRIQDLERIAGLGIKVLRYPVLWETIAPDHPDECDWTWHDARMYRLRSLGIEPIA
                     GLVHHGSGPRYTDLLDPRFGDLLAVHAERVATRYPWIRRFTPVNEPLTTARFSGLYGH
                     WYPHGRDTRTMLRCLYNECRAVALSMRAIRRVIPDAQLVQTEDMGKTFTLPDLKYQAD
                     YENERRWLGFDLLCGRIDAAHPWHREFLAAGIGERELAAFVDEPTVPDIIGINHYVTS
                     ERFLDRRKRDYPEAFHVADVPYVDVHATRARLADGLTGPIHRLREVWERYRLPIAVTE
                     THLGGSRDEQLRWLCQMWDAAQTLCSEGVDMRAVTVWSLFGCVDWNSLLMQDSGFYES
                     GVFDVRGSDVRPTALAAACKALATEGRFDHPVLDGPGWWQRAERFFDLPAQPVAAPAV
                     ANAARRLVITGASGTLGRAFSRVCADRGLAHVLLSRADMDIADQASVEAALKRWRPWA
                     VINTAGYVRVADAANESERCFRENTTGAEVLARSCAALGIPYVTFSSDLVFDGRLGRA
                     YVESDRVSPLCVYGSSKADAERRVLQAFPESLVVRTSAFFGPWDRYNFVHAALRELAA
                     GRPFHACAESLVSPTYVPDLVHAVLDLLIDREQGLWHLANQGMLSWHELAVRAARQAG
                     LDASALVMTHGGGRGVTALSSERGLILPSLSNALERYLRENTIAWEGNPATRAAA"
     gene            14790..16574
                     /locus_tag="VM406_08175"
     CDS             14790..16574
                     /locus_tag="VM406_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HVL75979.1"
                     /translation="MTETAYPRPQLVREHWLSLNGEWQFTFDSERKYRSPLDAIEWTR
                     RIRVPYPPESQASGIGDRGFHRACWYRREFEIEAGEGRVLLHFGAVDYRARVWVNGHL
                     VADHEGGHSSFHADITAALEGGKRHVVTVLAEDDPHDLSKPRGKQDWQLEPHSIWYPR
                     TTGIWQAVWLEKVPGTYIQRLRWTPHFDGFEIGFEAFVTGDECEDLTVEVKISHGEKV
                     LADDRYHVIGGEAHRKIALSDPGIDDSRNELLWSPERPTLLDAVVTLRSGETVLDTVR
                     SYTALRSVHVSRDRFMLNGRPYHLKLVLDQGYWPDTLLAAPNDEALRRDVELAKAMGF
                     NGVRKHQKIEDPRYLYWADRLGLMVWEEMPSSYRFTPTTINRMVREWSEVIERDYSHP
                     CIIVWVPFNESWGVPNLTSTQAHRNAVEALYHLTRTFDSTRPVIGNDGWEASATDLLG
                     IHDYDGDPERLKARYMVNPQELFDRRRPGGRILTLDGYPHRGQPIILTEFGGITYAKV
                     KEEGVEDVWGYSMAGSDQEFLETYRRLLEVVNESTLFSGFCYTQFADTFQEANGLLTA
                     DRTPKLPLEQIYAATRNIKPNQKMQYGI"
     gene            complement(16605..17300)
                     /locus_tag="VM406_08180"
     CDS             complement(16605..17300)
                     /locus_tag="VM406_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HVL75980.1"
                     /translation="MTHLDSPLSDREFHELDQFLLSDRCAEDGMTMDALHGYLTALAI
                     GPEEVPMAEWLPKVWGAYGEKGPQFSSPKEAQRITALIGRFMNEVLVTFEVAPKEFEP
                     LYCEHEHEGQPLLDADAWAWGFWEGMQLRAEAWQPIYDSELAQLMEPFYLLGAEEIEE
                     EEAQALEDPVKRHKLAIEMEAAIPHIHRFWKPRRRSTVQQVQRDAPKVGRNDACPCGS
                     GKKFKKCCGAEQV"
     gene            complement(17333..>17958)
                     /locus_tag="VM406_08185"
     CDS             complement(17333..>17958)
                     /locus_tag="VM406_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75981.1"
                     /translation="LVRTLRFILLSLALHLVLLSGLSWKSHVPLTLQPQRTAAQRLDV
                     HLLSPPPPEAVSTAHAPARPAPDTSQASAALSDRLAPAPPAPPPSAAAALPAPAVIAR
                     QTVEYMEPERLTRPPVPLARVRLNTREIRGVGFEGSVVLTLLVDRWGAADVLVPPGAG
                     LEEEFAALVAAQFRKVRFAPGEIDGQPVHSRVQITVISEPDLVQSDF"
BASE COUNT         3118 a         5637 c         5992 g         3211 t
ORIGIN      
        1 tttcatgatc tcgcgcctga agcctgaagg tgagctttcc ggcgaggagg cgcggcacgc
       61 atggcccggc ggcgcatgga aacttgggcg ccgcaagtgc cgttcgttca agcggttttc
      121 ttcgctgtgt cgtcgaggcg caggcgggca ccgctcaatt cgccgcgcgc ccggtgcgca
      181 cgtcgcttgc gccaccggcc cggatcgccg ggaaaatgcg catcaaacgc gaaaatatcg
      241 gccgtgctac gatgaatcgc ctcattggcc gcacctaccg cttctacctc gcatgcaaga
      301 gttgtgtaat gagccctttc tgcacctttg cctggatccg gatcaacgga tcctgtgcgc
      361 gcagtggaag ggctatcaaa gcttcgataa cggtgtggcg ggctgtcatc gcatcctcga
      421 cgaagcgacg aggcacggca tctcccgcat cctcaacgac aacaccctcg tcagggggct
      481 ctggatggat gccgccgaat gggcgggccg catctggttt ccgcgtctgc ggcaggccgg
      541 cgtgaagcat ttcgcgtggg tctgcagtcc cgctcgtttc tcgcgcatct cgatgagcgc
      601 ggcgcttgcc gtgatcgatg acaccgaact ggacctgcgc gtcttccaga ctgtggaaga
      661 ggcctccgtc tggctgcatt cggtgtccga cgcgtcgccg caatgacgag gtgacccaag
      721 gcatgagcgc catccaaaaa aacaggccgc atctgcggcc tgtctgcaat gtgcaagcgc
      781 ctggcgtgct cagtgcccga gaatcttcga cagaaaatcc ttgctgcgct cgttgcgcgg
      841 attgttgaag aactcgtgcg gcgtgttctc ctcgatgatc tggccctggt ccatgaagat
      901 cacgcggtcg gcgacggcct tggcgaagcc catctcgtgc gtcacgcaaa tcatggtgat
      961 gccctggccg gccagttcga tcatcacgtc cagcacttcc ttgatcatct ccgggtccag
     1021 cgccgaggtc ggctcgtcga acagcatgat ctttggcgtc aggcacagcg cgcgggcgat
     1081 cgccacgcgc tgctgctggc cgccggagag ctgcagcgga tacttgtgcg cctgttcggc
     1141 gatttgcacg cgctcgagct gctgcatcgc gcgcgcctcg gcatccttct tcggcatctt
     1201 cccgacccac atcggtgaca gtgtgaggtt ttccagcacg gtcaggtgcg ggaacaggtt
     1261 gaactgctgg aacaccatgc cgacgttctt gcgcacctgg tcgatggcct tcacgtcgtc
     1321 ggccagttca atgccatcga cgaccaggcg tccttcctga tgctcttcga gccggttgat
     1381 gcagcggatc agcgtggatt tgccggagcc ggaagggccg cagatcacga tgcgctcgcc
     1441 gcgcttgacc tgcaggtcga tgtcgcgcaa cacgtggaag ttgttgccgt accacttgtt
     1501 gactttttca aagctgataa tcggttcagt catggcttgt tccgtcttga cggttgacgt
     1561 ggcggccgcg ctcatcgctg gcgccccttg ttcacttgtt tctccaccca caggctgtag
     1621 cgcgacatcg caaagcagaa cgcgaagtag atcgccgcga tgaacagata gccctcgatc
     1681 ttgtacgggc gccagagcgc atccgaattg agcgcaaggc ccatcgagcc ggtcagctca
     1741 tacaggctca cgatggtcac cagcgaggtg tccttgaaga tcgcgatgaa gttgttcatg
     1801 atccccggca ccaccattgc cagcgcctgc ggcagcacga tcttgcgctg cgtctgccaa
     1861 tacgaaaggc cgagcgtcgc ggccgcctcg atctgaccct tcggaatcgc ctgcaagccg
     1921 ccgcggatca cctcggccat gtaggcggcg gcaaacagcg tgatgccgat cagcacgcgc
     1981 agcagcacat cgatcgtcat tccctccggc atgaacagcg ggaacatgaa cgaggccatg
     2041 aacagcaccg agatcagcgg cacgccgcgg atcagctcga cgtagatcgt gcacacggtg
     2101 cggatcgccg gcagctccga gcggcgaccg agcgccacca gcagcgcgat cgggaaggcc
     2161 atcacgatcg acagcgtcgc cagcaggatg gtcagcggca gcccgcccca acggtcggtg
     2221 tccaccggcg tcagtccgaa ccagccgccg gccatcagga cgtagaacgc ggccagtatg
     2281 acgacccaca ggcccgccag ccagggccgc cagaacgccc gcgtgcagct ggcgaccagc
     2341 agcaggacca tcaggatggt ggcaaccagc ggacgccatt gttcgtcgaa cgggtagcgg
     2401 ccgaagatga tcagccggta tttctcggcg atcacgcccc agcaggcgcc cacgccttcg
     2461 gcgcggcaca ggtccgagtc cggcgcccat accgcgcgga acagccccca gctgatcagc
     2521 tgagggatgt accagagaag caggccgccg acgatcagcg tgccgatcga ggttttccag
     2581 tcgccgaaca ggttcgcgcg ggtccagccg atcacgcctt cgtcgcgcat cggcgccggc
     2641 cgcgggggaa tgatgcggac cggcgcgggt acctggtcgt cgacgcgttg aatggtaggt
     2701 gtagtcatgt cgcgctcccg cttaacgttc cttgatcgcc gcgcgggcgt tgtaccagtt
     2761 catcagtccc gaagtcgtga gcgaggtggt caggtagacc agcatgatga tcgcgatgca
     2821 ctcgaccgcg cggccggtct ggttgatcgc cgtattggcg attgacacca cgtccggata
     2881 gccgatggcc accgccagcg acgagttctt cgtcaggttc aggtactggt tggtcagcgg
     2941 cggaatgatc acgcgcagcg cctgcggcag catcaccagg cgcatcgcct gtccgcgaga
     3001 caggccaagc gccgccgccg cctcgccctg gccgtgcgag accgactgca cgccggcgcg
     3061 gaccacttcg gccacgaagg cggcggtgta gatgaccagg ccgagcagca cggcgagaaa
     3121 ttccggcgtg agcgcgccgc cgccttcaac cgcgaagtcg ccgcgtcccg gcacttcgaa
     3181 ctgggtctcg gcgccgccgg ccagccagcc gagcacggtc atcgcgacga tgatcagcgc
     3241 gggtatgagg aagatgctgg ggggcttgcc ggtctcctcg aagcgcttgc ggctccacct
     3301 gcggtaaagc cacgccagcg cgatgccggc ggccagaccg gccagcggcc agatcacgtc
     3361 cttgtcccag atcggtatcg ggaagttcac gccgctcttg ctgaggaaga agggaccgac
     3421 ctcccaggct tcgtacaccg ccggcaggaa ctcggtgaag accaggtacc acatcagcag
     3481 ctgcagcagg atcgggatgt tgcggaatat ctcgacatag gcgccgcaga ccgagcggat
     3541 cagcgcgttg cgcgacagac gtccgatgcc gatcagcgtg ccgatgatcg tggtcaggat
     3601 gatgccgagg atggccacgc gcagcgtgtt ggccaggccg accaggaacg cgttccagta
     3661 cggatcgaac gagtcgtacg ggatgatgaa ctcgccgatg tcgaagccgg ccgtctgccc
     3721 gaggaagtcg aagccgctct ggatgccgcg gattcgcatg ttctgcgcgg tattgtgggc
     3781 aaggaaccag accgccagtc cgatcaggac gagcgcgacg atctggtaca cgaggcctcg
     3841 gaaagcttgg ctgcgccagg accaactctt ccgcttggcg ggtggtcggg agggtgtcat
     3901 ggtgggatcg cttcagagaa tgacaggcgc aaacgcaaga gcctccagca aaaaaggcgc
     3961 gcggatcccg cgggctgcgc tgctcgcctg atcggatgcg attgcgcgag tcggtccatg
     4021 gaccgctttt ttggtggagg ctctcgtttt gctgcgggct gcggaatcgg cgagcctggg
     4081 gcgcgccgat tccgagcggt gttccgttac cgtaagcgtg ggctcagcgg accggatagg
     4141 cgtacatcag gccgcccttg ttccactggt tgttcagacc gcgcggcagg cccagcgggg
     4201 acttcggacc gacattgcgc tcgaagattt caccgtagtt accggtggtc ttgatcgcgc
     4261 ggccgagcca gtccttgtcc aggccgagca ggcggccggt gtcttcggat gtgccgagca
     4321 ggcgctgcac gaccgggtcc tggctggtgc gcatctggtc gacattggcc tgcgtcacgc
     4381 cgaattcctc ggcttcgagc aagccgtaga tcacccattt cacgatcgcg aagagctcgt
     4441 cgtcgccacg gcgcaccatc gggccgagcg gttccttgga gatcagttcg ggcaggatca
     4501 cgtgctcggc cggcacgcgc gcttcctggt tgcgcaccga agccaggccc gacgcatcgg
     4561 tcgtgtaggc cacgcagcgg ccggcgaagt aggcgccggt tgccgcctcg accttctcga
     4621 acacgaccgg cttgatgttc aggccgttgg cgcgggaata gtcggtcagg ttcttttccg
     4681 tggtggtgcc ggactgcacg cacacggtct ggcccttcag ctgcttggcg ctcttgatgt
     4741 tggtcttcgc cggcaccatg aagccctggc cgtcatagta ggtgaccgcc gtggagtgca
     4801 gaccgaggga agcgtcgcgg gtcagggtga aggtggtgtt gcgcgacagg atgtcgactt
     4861 cgccggactg cagtgcggta aagcgctgct gggcggtcag cggaacatag cggaccttct
     4921 cgccgtcgtt gaggatggcg gcggctaccg cgcggcagat gtcgacatcg aggccggacc
     4981 agcgtccgtt ggaatcagcc tgggaaaagc cggccaagcc ggtgttgacg ccgcagacga
     5041 ggttgccgcg ctgcttgatc gaatcgagcg tgcggccggc atgggccgga agggacagca
     5101 gggttccgag ggtgaccacg gcggcggttg ccagcttgat cttgcttagt ttcatttgtc
     5161 gtctccaatt aagaggaaaa ccggatcttg acgtccgggt atggcggctc gcgccgatgg
     5221 ggagcatgct cctttctggc gctttcggca acacccgcga atgctagagg aacgcatcgc
     5281 agcaaagcta atgttttttg tgcatgtgcc catgcaaaaa aaacatgcct cttgcgctgt
     5341 ccgcgatacg tgttccagcg gtgagcagca tccgagcggg cggagcgcga ccccgtgggc
     5401 cgcgcgatcc ggccggtctt cattcttgct cattgaaccg acgtcggcat gatattcagc
     5461 aagagacgtg cctgtggaca atgcctgttt gtgccctgat ccggggaggc gctgcgtcat
     5521 ctgcgttcag gagcgcgcgc gaaagaggcc ggggtgcgcc catctggtgc gcacgcccag
     5581 gccgtggtgc ggcggttgcc cggaaactcg ggtgcaggct acttgaaacg atccgcgcgc
     5641 gggatcgacc tgccggtcca ggtgcgcggc ggcatttgca gttcgctgcc gccgagttcg
     5701 tcttctcccg cgcgcggttc gcgcaggcga tagacgctcg cccagatatt gatgcagcgg
     5761 gagatctcgg tccggtccag ccttgccttg cccggcttgg cgcccggacg cggcagcgga
     5821 ggccgtgctc cggagagttg gggggaattg accggcgctg cttgcttctc catgttgacc
     5881 tcgctccact gcatgtccgt gtcagtatag gaggccgctt gaagacttca acagcttccg
     5941 ccgtccgctt gatgcgaggc aaacggcgcg ggcgagcgcc catgaaaaag cccgccggga
     6001 accggtcccg cgggcaaggg ccaccattgg agaactgaag acccgattgc actatcggcg
     6061 ctcggggccg gcgagtccaa ccgggttacg caaggttaca tggcctactg cctgcgtccc
     6121 aggaagcccc ccatgatggt ctggccggta gccgggtggg tcagcatgaa ggcgccgccg
     6181 acctctgccg gtgcggccgt tcccatcccc gctgacggcc catagagacg cgcgctgatc
     6241 ccgccattca tcatcccggc cgcggcggac ccggtcaggt agttggcctc ggcatcgcca
     6301 ctgccggcca gtgcggccgt gaaggccgcc ggcaccggcg atcccgatcc gtcatacgtg
     6361 ttggtgtcct ggatcgtgac caccgcggtg cgcgtggcga agttcacctc gacacgcgcg
     6421 gtgccccagt acggtggcag ttcctcgaca tcctcacggc ccgcgtacca gccgtaggcg
     6481 atgccggtat aggtcgccga gccgaccgtt ggcacctcgg ccggtggcgt gacatcgccg
     6541 aaggaaacca cgccgcgccg cagcttgttg tgctccggag tggtggtcgc ccactcggcg
     6601 atcacaacgg tgtcctgggt cgtccaggtc gccaggttcg catagcggta gctccagttc
     6661 gtcatgtcgc ggtatccctt cgaggcaggg aaggggatgc cgaaaccggt gccgccgggc
     6721 agccaggtgc gattgccgtt cgcgtccggc ggtccatcgc cggcgtgcac gtcgccgtcc
     6781 gaatgaaagc gttgcacgga ggcgaagtcg ggagtgaggc cgccgttgtg ccagctcccg
     6841 ggcgggcggg cagccgttct tacgccgtcg gtttcggtag aggcgcaggt gccgggtccg
     6901 cccgggcagc gcggcggatt gttctctcgg gggaagtagc ggttgttcgc gtagcgggct
     6961 tgggtcgccg cgcgaccgag cgtggcgtaa tcgtagaaag cgagatcgga gctgtccggc
     7021 agtggaacga aggtgatccg accgtttgca tcgagttcga cacggccctg ggtcggattg
     7081 aaggtctcga agatcggggg ccgttcgtta acgccgtccc aggacaggcc gaggttgcgc
     7141 agcagcaggt agaaggcgct ggtgcttcct tcagcattgc ggcgcacgcg cacttcggcc
     7201 gggccgccgc tggccgggcg caggccccac gcgcgagtcg tgacggtatg gccaggatcc
     7261 agatcgctgg tcgaaatccc gtagacctgg accccggagt cggtcagcgc gacgccgttc
     7321 tccacgcgca gcaccgtggc cccgctgcac tggaagtcgg taccgtcgac acagtgcatg
     7381 gtcatcggcg tcgcctgctg cgggggcggg ttcgtgccgc caggcggcgg attcgtatcg
     7441 cccggcggat tgccgaacgg cggattgccg gaaggagtgt cggaggagcc gcctccgcag
     7501 gcgctcagcg cggcgaccag gagcagaggc aggaatttgc gggcggggtg tgcttgcatg
     7561 gacagtcttc gtaggtctga tattgctcgg cgacgaacgt cgcgagcgcc tgccatgaag
     7621 ttatcgccgt tgcatgcatg tccatttgcg cagcgataag ccgtcgtctt gccgcgccga
     7681 aggaaaggac gagcgttgcg cgccctcaat ctggcggggc gggaacgaac cgagtagaag
     7741 ggggacgcgg cgttgtgccg caggcgcggc gaactgtcag accttgcgga acatcttgcg
     7801 cgcaagcgtg cggcacatgc ggtcccagag gctggtcagc tgatggatga atcgggacga
     7861 accgctggag cggctgcggg agcgccgcga atagtatgcg gcgtgcgacg cgtgccggga
     7921 gtggcggccg ccgctctcgc ggcgcgtcac cggttcagcc gcggcaggcc cgtcctcgcg
     7981 ctcggcgcgg gcgatccatt catcgtgctc ctgccgtcgg cccgggtccg acaatacctc
     8041 gtaagcggta ttgatgagct ggatgatgcg gatggcttcc ttgctgttgc cgtggcggtc
     8101 gggatgatgt ttctgggaga gtgtcttgta ggccgcccga atgacctcgg gcggcgcatc
     8161 gcgcgccacc ttcaggttgt cgtaatgcgt gtgcactcgg ggcatgtgga cggatccgga
     8221 aagtctgagg ctcggtgact tgcgccggaa ggcgcatctg gcagtgccac tgcactaccg
     8281 tatcgctatc gtaggggcgc ttcaccttgc cactattgca aattatcaag cttcccaacg
     8341 cttgagcttg ccgcattgta accgcagcga ggattggcgt taatacatcg cttagccaga
     8401 cttgctgccg gtcttgttgt cggatcgatt tttcggggca ggggttccac gagagcgagc
     8461 ccgggacgac acgccgctgg cgggagccta caggctgcga gcgtaataaa tcgccggata
     8521 ccagaccgcg gtccagtact ccatcgtgga cttgatcatg tactcggcaa gcaggacttg
     8581 ggcgggcatc ggcgtctcct cattgtatga agctatgaag cgcccactgt aagcaagggg
     8641 ccgggcgccg actttgacgt gcctcaagcc gcgtttccac aggcaatcgg aatcatgtgg
     8701 gacctggcgt cgccgtgaac tcggcgcgca gcctggggag gtattgcggg tagctgcgct
     8761 gcacgaagtc gatgaggcct tcgcgcagtc ggcagcgcag atcccaggcc tttccggcgt
     8821 cggcggcgct gacgagcacg cgcaactgca tggcgcgctc gttggtgtcc accacctgga
     8881 tcaggcacag cctgccgtcc cactcgggcg ccgcagtgca gaccgcttgc gcctgtgtgc
     8941 gcagcacatc gagcggcatc cggtagtcga cccacaggaa gacggtcccg aggatgtccg
     9001 atgtgcggcg agtccagttc tggaacggat tctcgatgaa ccattgcagc ggcaccacga
     9061 gccggcgctg gtcccagatc cgcacgacca cataagtgcc ggtgatttcc tcgatctgtc
     9121 cccattcgtt gttgatgatg acgacgtcat cgaggcggat gggctgggtc agggcgatct
     9181 gcaggccggc cagcaggttg ccgaggaccg gcttggccgc gaagccgagc accagccccg
     9241 cgacaccggc cgacgccagc aggctggtgc cgacctggcg cagcaggggc agcgtcatca
     9301 gcgcgctcgc ggtgccgatc agtacgatca gcaccatcac cgagcgcgcc agaaccttgg
     9361 tctgcgtctg gatgcggcgc gcatggaagt tgtcgatggc gtcggccgga tgcagttcca
     9421 cgattgcatg cgcaatgccc ctgacgacgc acacgccgag ccaggtgagc gtgatcacca
     9481 tcgcgacggc gttcagatga cgcgccagct ccagcccggt gagatggggc ggggcattgc
     9541 gcaggatgat ctgcaccagc agcagaaaga acagcaggcc gcccgcgccg tgcgcgtagt
     9601 caacgacttg gcgcgaatat ggaagcgggg cggcaagccg ccgcagcatc gcggtggcgc
     9661 cggcatggac gaggagggcg accggcagcg cgatcaggac ctgcaccagc acgctgatcc
     9721 acggcaggcc gcgaatcagc acgatccatg gtgtgatcag atagacgatg gcgagcagcc
     9781 agtccggcat gcgttctgtc atgtcaggtg cggcgcgggc taggcagcgc tcacgaggct
     9841 ttcttctggc agggcgggtg gcgggtaaag gaccgggtga tggtcgggca cgggttcgtc
     9901 atccggagac gggttcttgt ccggcatcgg atcgtctccg ggaacgggat cgggcggcac
     9961 ctgatgcgct tcggcaggtg acatgcaagg ctcctgtaca gacacagggc tcatgctacg
    10021 cgctggatca atcgccagcc ttgcgattgc gcagaaccga ctgcggcaag gcgggatatg
    10081 agccaccatg cggtgcggga atgactgata ttcccaaact aaagctgacg acgcagcgca
    10141 tcgccgctaa agtgggaact gtctcctcca actcttcttg atttggattc gcccacagac
    10201 cgcaaggcct gtgggcattt tttttgcctc gccgaattgg aaagtgcagc gaatgagcag
    10261 ccgctccaaa cgctgcccga tcaaggactt gggcagggct ccacaaggtt ttccccaggc
    10321 ttgtgcacag ttcctgtggg taagccgagg cgctcaggaa gcgttcggct gcttcggctt
    10381 gaattgcttg tcgctgatgc ggaacttggc gcggcgatca cccatgagtt cacgcaggtc
    10441 cttgatgctc aactgggaaa cttcgtgcat gcggatcagg agcgacgccc cgaccggcag
    10501 gcggcgatgg cggatcttgc tgatcagcgg cggggccacc tcgagggcgc gcgacagtgc
    10561 tgcatcgttt ttcagattca ggcgctcgat cagcgattcc agcaggttgt tcggatcgta
    10621 ctgaaactgc tgctgttgct ctgcggcgga ggtctctttg gtttcgttca tctggctgtt
    10681 ccccaaggtg gttcgaactg tttcaactgt ttcttattgt ggtcaattgc ttcgatgatt
    10741 ttttgacaat tcgcaggaat gcgaagggct cggcctcgtg tctgcgttgc ctcagacgaa
    10801 atacgccgcg ccgagcacca gcggggagcc gctcacaccc gcgacgacca ggcgccgggc
    10861 gacgctgtgg atctggtcct cgaaggcgcg gtaggtctgc aggtaatcga gagagccgcc
    10921 tttgagccgc gccagatgag cgagggcatt tttcgaaacc acgcattcac ggtccgcgaa
    10981 tccgacttcc accatgaagg tcacgccttc ttcgccgaca cggggctcgg ttgttgttct
    11041 gggcatcagg gtctccattg tcatatcagt agtcagtcgt tgcagagagc ttgagccgat
    11101 agtagtaagt cgtacaagca acgtcttgac ggcagtcaat tcggtcgtgc ccgtcaaagc
    11161 ttttcattgc gggcaatcgg atcgcgtgtc cccgccgctg gccccagggg ctgctgcgtg
    11221 ggtctggatg catgcggagc gcttgagcgg gaacaaggtg accgcgccgc tgctgccgtt
    11281 tcgcacagaa tcgtccgcac gtgcgtcgct acagtggagg taaccgtaat tcgccgctgg
    11341 ggcggggagc tgccatgacg ccggaagaaa tcgttgccga tctcaatgca cgcaatcgcg
    11401 aagcgctcga cgcgcgcggc catgccggtt cgttcaccag cgaacaggtg ttggcgctga
    11461 tggacaaggc ggccatgcac ggtttcaagg tgggcagcaa tgtcgcgctt tcgatggtca
    11521 agggatcgct gctggtgcag ctcaatcgcg tcgccgcacg ccaggacaga cgccgcccct
    11581 gagcagcgcg ctgaaatatc tcgttacatt aaaaaccgct cgggcagcaa tctttccgga
    11641 attcaaaagc cgtaacatcc tcctcgtcac ctatgaggag gttggaaatg agcttgcatc
    11701 acaagcgtag tcagatactg gccgtgatgt tgctcaccgg cgcggtgccg tttgcatcgg
    11761 caaacggatt cgacgacttt gcccgcgtcg tgagcgtcac gccccagtac gagcaggtca
    11821 atcatccgcg ccaggaatgc gtgaccgagt acgtacaggt ccagcgaccg gcgcaggacc
    11881 gcggcatggc gggcgcagtc atcggcggaa ttgccggcgg tctgctgggc aatcaggtcg
    11941 gcggcggcag cggacgcacg gcggcaaccg cggcgggagc gatcgccggc accatcgtcg
    12001 gcgatcgcat cgagaacagc aaccagccgg gcaccgtcgt cgagcagcag cctgtgcagc
    12061 gctgccgtac cgtcgacaac tggcagaccc gagcgaacgg cttttccgtc acctacgaat
    12121 accgtggctt catgcacacg accacactgc cgaatcaccc gggcgaacgt ctgcctgtgc
    12181 gcgtgtcggt caccccccgc atgtaggcag tcttgccatt cgccagcggc gcctgcgggc
    12241 gccgttttca tttggctgat gcatttcaaa gtgcgggcac gcgggcgcgg gcagagcgga
    12301 accttcagca ctgcgagccg gtccagaagc gtggacaaac ccgcaatgcg ttgccaggcg
    12361 tcatgtcgcg gccgcgcatc ctgcatgcag agaagatcca acaacaaccg ctcgcccggg
    12421 ggccccgggc ggtttttcat ttcctcctga ccggcactcc ctgggcgtca ccggtcttca
    12481 gtcgtgccgg tcgtcctgcc gcaaaacgca tggacaagca actgaaaccc gttcccgact
    12541 cccatcaacc cgaccgccgc caaccgctcg aactgtgggg cggcatggaa tgcacggtcg
    12601 cgcgcctggg cgaccggttc cgcgatcaat tgcgcgaaac cggccatgcg cagcgcatcc
    12661 aggatctcga gcgcatcgcc ggcctgggca tcaaggtgct gcgctatccg gtgctgtggg
    12721 aaacaattgc tccggatcac cccgacgagt gcgactggac ctggcacgac gcgcgcatgt
    12781 atcggctgcg ctcgctgggc atcgagccga tcgccggcct tgtccatcac ggcagcggtc
    12841 cgcgctacac cgatctgctc gacccgcgct tcggtgatct gctcgccgtt cacgccgagc
    12901 gcgtcgccac gcgctatccg tggatccggc gctttacccc ggtcaacgag ccgctgacga
    12961 ccgcccgctt ctcgggtctg tacggccact ggtatcccca tggacgcgat acgcggacga
    13021 tgctgcgctg cctgtacaac gaatgccgcg cggttgccct gtcgatgcgg gcgatcaggc
    13081 gcgtgatccc ggatgcgcag ctggtgcaga ccgaggacat gggcaagacc ttcaccctcc
    13141 cggatttgaa gtaccaggcc gattacgaga acgagcggcg ctggctcggc ttcgacctgc
    13201 tttgcgggcg gatcgacgct gcgcacccgt ggcatcgcga gtttctcgcg gccggcatcg
    13261 gcgaacgcga gctggccgct ttcgtggacg agcccactgt gccggacatc atcggcatca
    13321 atcattacgt gacgagcgag cgcttcctcg accggcgcaa gcgcgactat ccggaggcgt
    13381 tccatgtggc cgacgtgccc tacgtggacg tgcatgcgac gcgcgcgcgg ctggccgacg
    13441 gactgaccgg gccgattcat cggctgcgcg aagtgtggga acggtatcgc ctgccgatcg
    13501 cggtgaccga gacccacctt ggcggcagcc gcgacgaaca gctgcgctgg ctgtgccaga
    13561 tgtgggatgc cgcacagacc ttgtgctcgg agggcgtcga catgcgcgcc gtgacggtgt
    13621 ggtcgctgtt cggctgcgtg gactggaact cgctgctgat gcaggacagc ggtttctacg
    13681 agagcggcgt gttcgacgtg cgcggctccg acgtgcggcc gaccgccctt gccgcggcgt
    13741 gcaaggcgct tgcgaccgaa gggcgcttcg accatccggt gctcgatggc ccgggctggt
    13801 ggcagcgtgc ggagcgcttc tttgacctgc cggcgcagcc ggttgcggcg ccggcggtcg
    13861 cgaacgctgc gcgcaggctc gtgataaccg gcgccagcgg cacgctcggg cgcgcattct
    13921 cgcgcgtctg cgccgaccgc gggcttgctc atgtgctgct gagtcgcgcg gacatggaca
    13981 tcgccgatca ggcgagcgtg gaggccgcgc tcaaacgctg gcgcccctgg gcggtgatca
    14041 acaccgccgg ctatgtacgc gtggcggatg cggccaacga atccgagcgc tgcttccggg
    14101 aaaacacgac cggcgccgaa gtgctggcac gcagctgtgc cgcgctgggc attccgtatg
    14161 tcaccttctc gtccgacctg gtcttcgacg ggcgcctggg ccgcgcgtat gtcgagagcg
    14221 accgcgtttc cccgctgtgc gtgtacgggt ccagcaaggc cgatgccgaa cgccgcgtgc
    14281 tgcaggcatt tcccgaatcg ctggtcgtgc gcaccagcgc gtttttcggt ccgtgggatc
    14341 gctacaactt cgtgcatgcg gcgctgcgcg aacttgccgc cggacgcccg tttcatgcct
    14401 gcgccgaaag cctcgtatcg ccgacctatg taccggatct cgtgcatgcg gtgctcgatc
    14461 tgctgatcga ccgcgagcag ggcctctggc acctggccaa tcagggcatg ctgtcctggc
    14521 acgaactggc cgtgcgggcc gcgcgccagg ccggcctgga tgcgtcggcg ctggtcatga
    14581 cgcatggggg cggccgcggc gtgactgccc tgtccagcga acgcggtctg atcctgcctt
    14641 cactgtcgaa tgcgctcgag cgctacctgc gcgagaacac gatcgcctgg gagggcaatc
    14701 ccgccacgcg tgccgccgcc tgacggaacg ccattccctg cgcgcactcc aagccgcagg
    14761 caaagcgcga caacgacaag ggacagttca tgacagaaac ggcctacccg cgcccgcaac
    14821 tggtgcggga gcactggctg tcgctcaatg gcgagtggca gttcactttc gatagtgagc
    14881 ggaaataccg gtctccgctg gacgcgatcg aatggacgcg gcgtatccgc gtaccgtacc
    14941 cgcccgagtc gcaggccagc ggcatcggcg accgcggatt ccaccgtgcc tgctggtatc
    15001 gccgcgaatt cgagatcgag gcaggggaag ggcgggtgct gctgcatttc ggtgcggtcg
    15061 actatcgcgc ccgcgtctgg gtcaacgggc acctggtggc ggaccacgaa ggcggccaca
    15121 gttcctttca cgccgacatc acggcggcgc tggagggggg caagcgccat gtggtgaccg
    15181 tgctcgccga ggacgatccg cacgacttgt ccaagccgcg cggcaagcag gactggcagc
    15241 tcgagccaca ctcgatctgg tatccgcgca ccaccggcat ctggcaggcg gtatggctgg
    15301 agaaggtgcc gggcacctat atccagcgcc tgcgctggac gccgcacttc gacggtttcg
    15361 aaatcggctt cgaggccttc gtgacgggcg acgaatgcga ggacctgacc gtcgaggtca
    15421 agatctcgca cggcgagaag gtgctggcgg acgatcgcta ccacgtgatc ggcggagagg
    15481 cgcaccgcaa gatcgcgctg tccgatccgg gcatcgacga ctcgcgcaac gaactgctgt
    15541 ggagccccga gcggccgacc ctgctcgacg cggtggtgac gctgcgcagc ggcgagacgg
    15601 tgctcgacac cgtgcgctcc tacaccgcgc tgcgctcggt gcacgtgagc cgtgaccgtt
    15661 tcatgctcaa cggccgtccg taccacctca agctcgtgct cgaccaaggc tactggccgg
    15721 acacgctgct ggccgcaccg aacgacgaag cactcaggcg cgacgtcgag ctggccaagg
    15781 cgatgggatt caacggcgtg cgcaagcacc agaagatcga ggacccgcgc tacctttact
    15841 gggccgacag gctgggcctg atggtgtggg aggaaatgcc gtcgtcctac cgcttcacgc
    15901 cgacgacgat caaccgcatg gtgcgcgagt ggagcgaggt gatcgagcgc gactacagcc
    15961 atccatgcat catcgtctgg gtgccgttca atgaatcctg gggcgtgcct aacctgactt
    16021 ccacccaggc gcaccgcaac gcagtggaag cgctgtacca cctgacgcgc accttcgatt
    16081 cgacccggcc ggtgatcggc aacgacggct gggaagcctc cgccaccgac ctgctcggca
    16141 ttcacgacta tgacggcgat cccgagcgcc tgaaggcgcg ctacatggtc aatccgcagg
    16201 agctgttcga ccggcggcgt ccgggcgggc gcatcctcac gctcgacggc tacccgcatc
    16261 gtggccagcc tatcatcctg accgagttcg gcggcatcac ctatgcaaag gtcaaggaag
    16321 agggcgtgga ggatgtctgg ggctattcga tggccggcag cgaccaggaa ttcctcgaga
    16381 cctaccggcg tctgctcgag gtggtcaacg aatccacgct gttctcgggc ttctgctata
    16441 cccagttcgc cgacaccttc caggaagcca acggcctgct gaccgcggat cgcacgccga
    16501 agctgccgct ggagcagatc tacgcggcca cgcgcaacat caagccgaat cagaagatgc
    16561 agtacgggat ttagacgagc cgggacagcg ccgcatcaag acgttcagac ctgctccgcc
    16621 ccgcagcact tcttgaactt cttcccgctc ccgcaaggac aggcgtcgtt gcggccgacc
    16681 ttcggcgcgt cgcgctggac ctgctgcacg gtcgagcgcc ggcgcggttt ccagaatcgg
    16741 tggatgtgcg gaatcgccgc ctccatctcg atcgccagct tgtggcgctt gaccggatct
    16801 tcgagcgcct gggcttcctc ttcctcgatt tcctctgcgc cgagcaggta gaacggctcc
    16861 atcagctgcg cgagctcgga gtcgtagatc ggctgccagg cctcggcgcg cagctgcatg
    16921 ccctcccaga agccccaggc ccaggcatcc gcatccagca gcggctggcc ctcgtgctca
    16981 tgctcgcagt aaagcggctc gaattccttc ggcgccactt cgaaagtcac cagcacctcg
    17041 ttcatgaagc gtccgatcag ggcggtgatg cgctgcgcct ccttcggcga gctgaactgc
    17101 ggccctttct cgccgtacgc gccccacacc ttcggcagcc actctgccat cggcacttcc
    17161 tccggcccga tcgccagcgc cgtcaggtag ccgtgcagtg cgtccatcgt catgccgtct
    17221 tcggcgcaac ggtcggagag gaggaactgg tcgagttcgt ggaattcgcg gtcggagagg
    17281 ggggagtcga ggtgcgtcat gaatgcgggt tcggtcgttg ggcggactgc ggtcaaaagt
    17341 cggactgtac gaggtccggc tcgctgatga ccgtaatctg tacccgggaa tggacgggtt
    17401 gtccatcgat ttcgcccggc gcaaagcgca ccttgcgaaa ctgcgcggcg acaagcgcgg
    17461 cgaactcttc ctcgagaccc gcgccgggag ggaccaggac gtccgccgct ccccatctgt
    17521 cgaccagaag ggtgagcacg acgctgcctt cgaatccgac gccgcggatc tcccgggtgt
    17581 tcaggcgcac gcgtgcaagc ggcacgggtg gacgagtgag ccgctccggt tccatgtact
    17641 caacggtttg ccgggcaatc accgcaggtg ctgggagcgc cgcggcggcg gaggggggcg
    17701 gagccggtgg cgcgggtgcc agccgatcgg aaagcgctgc gctggcttgc gaagtgtctg
    17761 gcgccggtct ggcaggcgca tgcgccgtgc tcaccgcctc tggtgggggc ggggagagaa
    17821 gatgaacgtc gagccgttgg gccgccgtgc gttggggctg gagcgtgagc gggacatgtg
    17881 atttccagct caggccgctc agcaacacca ggtgcagggc gagcgacagc aggatgaagc
    17941 gcagggtacg cacaagca
//
LOCUS       DATLGF010000268         5327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_27661,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000268 DATLGF010000000
VERSION     DATLGF010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27661"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VM406_08190"
     CDS             complement(<1..444)
                     /locus_tag="VM406_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL75982.1"
                     /translation="MPAPLIALRLLRPLLAVVRVLWAAPITIFGLFIALPVRACGGHW
                     RRLRGGSTAAMLVHGRLADWLLERHPAGRMNAMAIGHIVVAARTGLGPLTLAHELEHV
                     RQAERWGPLFPILYAGSSAWQWLNGRNAYWHNHFEVAARAAADRAA"
     gene            514..1092
                     /gene="ruvA"
                     /locus_tag="VM406_08195"
     CDS             514..1092
                     /gene="ruvA"
                     /locus_tag="VM406_08195"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HVL75983.1"
                     /translation="MIGRLSGTLLEKNPPQILIDCNGVGYEVDVPMSTFYNLPAVGEK
                     TVLLTHLAVREDAHLLYGFGSAEERNVFKQLVKISGIGARTALSILSGMSVNDLAQAV
                     TLQEAGRLTRVPGIGKKTAERLLLELKGKLGADLGVAGVTAVSDASADILNALLALGY
                     SDKEAGAALKQVAPGAGVSEGIKQALKALSRA"
     gene            1103..2167
                     /gene="ruvB"
                     /locus_tag="VM406_08200"
     CDS             1103..2167
                     /gene="ruvB"
                     /locus_tag="VM406_08200"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HVL75984.1"
                     /translation="MSIQTDNFSEQRIIAATPASSNEEAIERALRPKQLHDYVGQEKI
                     HSQLEIFIQAARQRGEALDHTLLFGPPGLGKTTLAHIIAREMGVNLRQTSGPVLERAG
                     DLAALLTNLEPNDVLFIDEIHRLSPMVEEILYPALEDYQIDIMIGEGPAARSVRLDLQ
                     PFTLVGATTRAGMLTNPLRDRFGIVARLEFYTPQELARIVTRSASLLNAAIDDDGALE
                     IAKRSRGTPRIANRLLRRVRDYAEVKGRGEITKTMADAALRMLDVDPVGFDVMDRKLL
                     EAVLFKFNGGPVGLDNLAAAIGEERDTIEDVLEPYLIQQGFLQRTPRGRIAAPAAYAH
                     FGVIAPRSASSGDLWGDPPA"
     gene            2389..3219
                     /locus_tag="VM406_08205"
     CDS             2389..3219
                     /locus_tag="VM406_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HVL75985.1"
                     /translation="MLMSTQAHRFAHQLLEARAQGVQMPPISASGPLSVGDGYEIAKR
                     IMDIRIAQGETLIGRKIGFTNRRIWSRYGVNEPISNPIWTPIFEETVRFAEDNRGIQG
                     LAGAMQPRLGPELVFRLGRTPAPDATLEDLANCIEWMAHAFEIVTCPFPDWKFEMPDS
                     IAAFGLHGTLIVGEPRVLSAASRRNLADVLAHASLSISCGDELRSAGFGSDVLGSPLH
                     ALWHLHQLLRDLPQFQQLTEGEVITTGTWTDVCPVQAGETWTSAFSGITLPGLTVSFV
                     "
     gene            complement(3229..3681)
                     /locus_tag="VM406_08210"
     CDS             complement(3229..3681)
                     /locus_tag="VM406_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HVL75986.1"
                     /translation="MFRTWMTVAIAAFVLPAGGAWAQDPAEGLWRTIDDATGKPRSLV
                     RISENNGVLSGRVVKLFREPGEDPNPICEKCENSRRNQPVLGLTILTDMRREGREYTG
                     GEILDPSNGKVYKARMALSENGSKLDVRGYIGMPMLGRTQTWERVQEQ"
     gene            complement(3750..>5327)
                     /locus_tag="VM406_08215"
     CDS             complement(3750..>5327)
                     /locus_tag="VM406_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HVL75987.1"
                     /translation="IQDWCISRQLWWGHQIPAWYDDEGNIYVARDEDEAKAQAGGKTL
                     RREADVLDTWFSSALVPFSSLGWPEETPDYKLFLPSSVLVTGFDIIFFWVARMVMMTT
                     HFTGKVPFETVYVHGLVRDASGQKMSKSKGNTLDPIDLIDGIGIEELVRKRTTGLMNP
                     KDAHKIEKATRREFPNGIPAYGTDALRFTMASYASLGRNINFDLNRCEGYRNFCNKLW
                     NATRFVLMNTEGKDCGLDPSTCGPEGRDFSQADRWIVSLLQRTEAEVEKGFADYRFDN
                     IASAIYKFVWDEYCDWYLEVAKVQIQNGSEAQQRATRRTLLRVLETVLRLAHPIIPFI
                     TEALWQTVAPLAGRRPEGEASIMLQPYPQPNPEKIDEQAETWMAQLKAMTDACRNLRG
                     EMQLSPAQRIPLYIEAAGGEQEAMRAFAPYLQALAKLSEVQIVAALPQSPAPVSIVGT
                     VKLMLKVEIDVAAERERLGKEIARLEGEIAKANAKLGNESFVSRAPAQVVAQEKERVA
                     NFSATLVKLREQFEKLQ"
BASE COUNT          921 a         1728 c         1675 g         1003 t
ORIGIN      
        1 tgcggcgcgg tcggccgccg cacgcgccgc gacttcgaaa tgattgtgcc agtaggcatt
       61 gcgcccgttc agccactgcc aggcgctgga gcccgcatac aggatcggaa acagcggccc
      121 ccagcgttcc gcctggcgca catgctcgag ttcgtgggcg agggtcagcg gccccagtcc
      181 ggtgcgcgcg gctaccacga tatgaccgat tgccatcgcg ttcatgcgtc ctgccgggtg
      241 ccgttccagc agccagtccg cgagtcggcc atggaccagc atcgccgcag tcgatcctcc
      301 gcgcagccgg cgccaatgcc cgccgcaggc gcgcactggc aacgcgatga aaaggccgaa
      361 tatggtgatc ggcgcagccc atagcacccg taccacggcg aggagaggac gcagcaggcg
      421 cagggcgatc agaggggcgg gcatcggcaa atccagtgtg gcgtgcgcgg aatgtcctgt
      481 atgattcaac agtctttttt tgattccctg cccatgatcg gccgcctctc cggtaccctg
      541 ctcgaaaaga atccgccgca aatcctgatc gattgcaatg gcgtcgggta tgaggtcgat
      601 gtgccgatga gcacgttcta caacctgccc gcggtcggcg agaaaaccgt gctgctgacc
      661 cacctcgcgg tgcgcgaaga tgcacatctg ctgtacggtt tcggtagcgc cgaggagcgc
      721 aacgtcttca agcagctggt caaaatctcc gggatcggcg cgcgcacggc gctgtcgatc
      781 ctgtcgggaa tgtcggtgaa cgacctcgcg caggcggtca ccttgcagga ggccgggcgg
      841 ctcacgcgcg ttcccggcat cggcaagaag accgccgaac gcctgctgct cgaactgaag
      901 ggcaagctgg gcgccgatct cggcgtggcc ggcgtgacgg cagtcagcga cgccagcgcc
      961 gacatcctca atgcgttgct ggcccttggc tattcggaca aggaggcggg ggctgcgctc
     1021 aagcaggtgg cgccgggagc gggtgtatcc gaaggcatca agcaggcgct caaagccctg
     1081 tcgcgcgcct gattgaacga acatgagtat ccagaccgac aatttttccg agcagcgcat
     1141 catcgcggcc acgcccgcct ccagcaacga ggaggcgatc gagcgcgcgc tgcggcccaa
     1201 gcagctgcac gattacgtcg gccaggagaa gatccacagc cagctcgaaa tcttcatcca
     1261 ggccgcgcgc cagcgcggcg aggcgctcga ccacacgctg ctgttcgggc cgccgggact
     1321 gggcaagacc acgcttgcgc acatcatcgc ccgcgaaatg ggcgtgaatt tgcgccagac
     1381 ttccggtccg gtactcgagc gagccgggga cctggccgca ttgctgacca acctcgagcc
     1441 gaacgatgtg ctgttcatcg atgaaattca ccggctgtcg ccgatggtcg aggagatcct
     1501 gtaccccgcg ctggaggatt accagatcga catcatgatc ggcgaagggc cggcggcacg
     1561 ctcggtgcgg ctggacctgc agccgttcac gctggtgggc gccaccacgc gcgccggcat
     1621 gctgaccaac cccttgcgcg accgtttcgg aatcgtggcc cggctggagt tttacacccc
     1681 gcaggaactg gcgcgtatcg tcacccgcag cgcgtcgctg ctcaatgcgg ccatcgacga
     1741 cgacggcgcg cttgaaatcg ccaagcgcag ccgcggaacg ccccgcattg ccaaccggct
     1801 gctgcgccgc gtgcgtgact atgccgaggt caaggggcgg ggggaaataa cgaagacgat
     1861 ggccgacgcc gcgctcagga tgctcgacgt ggacccggtc ggcttcgacg tgatggaccg
     1921 caagctgctc gaagcggtgc tgttcaagtt caatggcggc ccggtcgggc tcgacaacct
     1981 cgccgcggcc atcggcgagg agcgcgacac gatcgaggac gtgctcgagc cgtatctgat
     2041 ccagcaaggc ttcctgcaac gcaccccgcg cggtcgcatc gccgcgcccg ccgcctatgc
     2101 ccacttcggc gtcatcgccc cgagaagcgc atcgagcggc gacctgtggg gagacccgcc
     2161 cgcctgagtt gccagtattt catcgtagaa atacggtatc gcgggaattc ctgaaagtcg
     2221 atttcccggt tcgccctata atgaccgacg gttaagcacg gtcgcacttt tcctcgttga
     2281 tttttcgcaa accggcaacg ccggggctgc gtaaagtaca tggctggccg gctgtcgtgc
     2341 agcaacagct gctccctcct ctccgaattc tgtcccgcgg gtatcgccat gctcatgtcc
     2401 actcaggccc accgctttgc gcatcagctg ctcgaggcgc gcgcccaagg ggtgcagatg
     2461 ccgccgattt cggccagcgg ccctctttcg gttggggacg gctatgagat cgccaagcgc
     2521 atcatggaca tccggatcgc gcaaggtgag acgctgatcg gacgcaagat cggcttcacc
     2581 aaccggcgca tctggtcacg ctatggcgtc aacgagccca tttccaaccc gatctggacc
     2641 ccgatcttcg aagaaaccgt ccggttcgcg gaagataatc gcggcatcca gggcctcgcg
     2701 ggtgcaatgc agccccggct tgggccggaa ttggttttcc gtctcggacg cacccctgcg
     2761 ccggatgcaa cgctcgagga tctggccaat tgcatcgaat ggatggcgca tgcgttcgaa
     2821 atcgttacgt gcccgtttcc cgactggaag ttcgaaatgc cggattccat cgcggccttc
     2881 ggcctgcacg gcacactgat tgtcggcgag ccgcgcgtgc tgtcggccgc gagccgccgc
     2941 aaccttgccg acgtgctggc ccacgcgagc ctttctatct cctgcggcga tgagctgcgt
     3001 tcggcggggt tcggcagcga cgtgcttggc agcccgctgc atgcactgtg gcatctgcac
     3061 caactgctgc gcgacctgcc gcagttccag caactgacgg aaggcgaggt catcacgacc
     3121 ggaacgtgga cggatgtgtg tccggtgcag gcgggcgaga cctggacctc ggcgttctcg
     3181 ggcatcaccc tgccgggcct gacggtttcc ttcgtctagc cgcgcgatct actgttcctg
     3241 cacccgctcc caggtctggg tgcggcccaa catcggcatg ccgatatagc cgcgcacgtc
     3301 gagcttgctg ccgttttcgg acagcgccat cctggccttg tagacttttc cgttcgaggg
     3361 atcgaggatt tcgccgccgg tgtattcccg cccttcgcgc cgcatgtcgg tcaggatggt
     3421 caagccgagt accggttgat tgcggcggga gttctcgcac ttttcgcaga tcggattggg
     3481 gtcttctcca ggctcgcgga acaacttcac gacgcggccc gacagcaccc cgttgttttc
     3541 cgaaatgcgg accagcgaac gcggcttgcc ggttgcgtca tcgatcgtgc gccacagtcc
     3601 ttctgccgga tcctgcgccc aagctcctcc tgcaggcaga acgaaggcgg caatcgccac
     3661 cgtcatccaa gttctgaaca tcggtccact ccctgctcgt gccttgcatt tttgataaag
     3721 cacgagtgtc catgggatgc ggccgcgcgc tattgcagtt tctcaaactg ctcgcgcagt
     3781 ttgacgagcg tcgcagagaa gttggcaacg cgttcttttt cctgcgcaac gacttgcgcc
     3841 ggggcacggg acacgaagct ttcgttgccc agcttcgcgt tggccttcgc gatttcccct
     3901 tccagtcgcg cgatttcctt cccgaggcgt tcgcgctcgg cggcgacatc gatctcgacc
     3961 ttcagcatca gcttgacggt gccgacgatg gagaccggag ccggcgactg cggcagcgcc
     4021 gcgacgatct gcacctcgga cagtttcgcc agcgcctgca ggtagggcgc gaacgcgcgc
     4081 atcgcttctt gttcgccgcc ggcggcttcg atgtacagcg ggatgcgctg cgcgggcgac
     4141 agttgcattt cgccgcgcag gttgcgacag gcgtcggtca tcgccttcag ctgtgccatc
     4201 caggtttccg cctgttcgtc gatcttctcc gggttcggct gcggataggg ctgcagcatg
     4261 atcgacgctt cgccttccgg cctgcggccc gcgagcggtg cgaccgtctg ccacagcgct
     4321 tcggtgatga acggaatgat cggatgcgcg agccgcagca cggtctccag cacgcgcaac
     4381 agcgtacgcc gggtggcgcg ctgctgcgct tcgctgccgt tctggatctg caccttcgcc
     4441 acttccagat accagtcgca gtactcgtcc cagacgaact tgtagatggc cgaggcgata
     4501 ttgtcgaagc ggtagtcggc gaatcccttc tcgacttcgg cttcggtacg ctgcagcagc
     4561 gagactatcc agcggtcggc ctgcgagaaa tcgcggcctt cgggtccgca ggtggatgga
     4621 tccaacccgc agtccttgcc ttcggtattc atcagcacga agcgcgtcgc attccacagc
     4681 ttgttgcaga aattgcgata gccttcgcag cggttcaggt cgaagttgat gttgcgccca
     4741 agcgaggcgt agctcgccat cgtgaagcgc agcgcgtcgg tgccgtaggc gggaatcccg
     4801 ttcgggaatt cgcggcgcgt ggctttctcg atcttgtgcg catccttcgg attcatcagc
     4861 ccggtggtgc gtttcctgac cagttcttcg atgccgatcc cgtcgatcag gtcgatcggg
     4921 tccaatgtat tgcccttgga cttcgacatc ttctggcccg aggcgtcgcg cacgaggcca
     4981 tgcacataga cggtctcgaa cggcaccttg ccggtgaaat gggtcgtcat catgaccatg
     5041 cgcgcaaccc agaagaaaat gatgtcgaag ccggttacca gcaccgagga gggcaggaac
     5101 agcttgtagt ccggcgtttc ttccggccag ccgagcgagg agaacggcac cagcgcggac
     5161 gagaaccagg tgtcgagcac gtccgcctcg cgccgcaggg tcttgccgcc ggcctgggcc
     5221 ttcgcctcgt cctcgtcgcg cgcgacgtag atgttgcctt cgtcgtcata ccaggcaggg
     5281 atctggtggc cccaccacag ctggcgcgag atgcaccagt cctggat
//
LOCUS       DATLGF010000269         3407 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_58501,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000269 DATLGF010000000
VERSION     DATLGF010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58501"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="VM406_08220"
     CDS             complement(<1..931)
                     /locus_tag="VM406_08220"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HVL75988.1"
                     /translation="MNDKLSPTLPAASPAAWLGVMGGGQLGRMFVHAAQKMGFKVAVL
                     EPARDCPAGDAADHLLTADYADEAALAELALQCAAVTTEFENVPAQSLAFLGERIFAA
                     PSSACVSIAQDRLEEKRFFTRCADTSGVLPAPYHAVLSDADVDCIPDALFPGILKTVR
                     MGYDGKGQVRVADRDEARVAFARMQGVPCVLEKMLPLAYEISVLTARGADGASVVYPI
                     AENVHRDGILFTTTVPGPNVCGERAEKAQQAALTIVGQMDYVGVLCIEFFVLQDGSLV
                     VNEMAPRPHNSGHYTIDACVTSQFAQQVRAMARL"
     gene            complement(928..1425)
                     /gene="purE"
                     /locus_tag="VM406_08225"
     CDS             complement(928..1425)
                     /gene="purE"
                     /locus_tag="VM406_08225"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HVL75989.1"
                     /translation="MAQSDKPLVGVVMGSSSDWDVMQHAVSLLKEFGVPHEAKVVSAH
                     RMPDEMFAYAESARARGLRAIIAGAGGAAHLPGMIAAKTIVPVLGVPVPSKYLRGEDS
                     LLSIVQMPKGIPVSTFAIGEAGAANAALAAIAMLATTDEQLAARLSDFRMKQTQAAQA
                     MTLPE"
     gene            complement(1593..2489)
                     /locus_tag="VM406_08230"
     CDS             complement(1593..2489)
                     /locus_tag="VM406_08230"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HVL75990.1"
                     /translation="MDHLYQSTITSLPLLGRGKVRDNYAVGNDKILIVTTDRLSAFDV
                     VMNEPIPGKGKVLNQMSNFWFEKLAGIVPNHLTGTAPESVVAGDEVEQVRGRAVVAKR
                     LKPILVEAVVRGYLIGSGWKDYQAGGAVCGIALPPGLRQADKLPEPIFTPAAKADIGE
                     HDENISYAEMENRIGKELAAKIRDISIQLYKTAADYAATRGIIIADTKFEFGLDENGV
                     LHLMDEVLTADSSRFWPADSYAPGSSPPSFDKQFVRDYLESLKHWDKAPPAPALPPDV
                     IEKTRVKYLEALQRLTGRALED"
     gene            complement(2748..>3407)
                     /locus_tag="VM406_08235"
     CDS             complement(2748..>3407)
                     /locus_tag="VM406_08235"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HVL75991.1"
                     /translation="GVTVEAELGVLGSLETMKGDKEDGHGAEGTMTREQLLTDVNQAA
                     DFVKQTQCDALAIAIGTSHGAYKFSRKPTGDILAIQRIKEIHARIPNTHLVMHGSSSV
                     PQELLAEIRQFGGDMKETYGVPVEEIQEGIKHGVRKINIDTDIRLAMTGAIRRFFAEN
                     PSKFDPREYLKPAREAAKGICKSRFIAFGCEGQAGKIKPIPLEKMAEKYKKGELAQIV
                     Q"
BASE COUNT          551 a         1110 c         1111 g          635 t
ORIGIN      
        1 gcaggcgcgc catcgcccgc acctgctggg cgaactggct ggtgacgcag gcgtcgatcg
       61 tgtaatggcc gctgttgtgc gggcgcggcg ccatctcgtt gacgaccagc gagccgtcct
      121 gcagcacgaa aaattcgatg cacagcacgc ccacgtaatc catctgcccg acgatggtca
      181 gcgctgcctg ctgggccttc tcggcgcgtt cgccgcacac gttgggaccg ggcaccgtcg
      241 tcgtgaacag gatgccgtcg cggtgcacgt tttcggcgat cgggtagacc accgaggcgc
      301 cgtccgcgcc acgcgcggtg agcacggaga tctcgtaggc cagcggcagc atcttttcga
      361 gtacgcaggg cacgccctgc atgcgcgcga acgcgacacg cgcctcatcg cgatcggcca
      421 cgcgtacctg gcccttgccg tcatagccca tgcgcacagt cttcaggatg ccggggaaaa
      481 gcgcatcggg aatgcagtcc acgtcggcgt ccgacagcac cgcatggtac ggcgccggca
      541 gcacgccgga cgtgtcggcg cagcgggtga agaagcgttt ttcctcgaga cggtcctggg
      601 cgatcgagac gcaggccgaa gacggcgcgg cgaagatgcg ttcgccgagg aaggccaggc
      661 tttgcgccgg cacgttctcg aattccgtgg tcaccgccgc gcattgcagc gccagctccg
      721 ccagtgccgc ttcgtcggcg tagtcggccg tcagcaggtg gtccgccgca tcgccggccg
      781 ggcagtcgcg cgccggttcc agcaccgcga ccttgaagcc catcttctgg gcggcgtgca
      841 cgaacatgcg gcccagctgg ccgccgccca tcacgcccag ccaggctgca ggcgaagccg
      901 cgggcagcgt gggagaaagc ttgtcgttca ttccgggaga gtcattgcct gcgcggcctg
      961 tgtctgcttc atgcggaagt ccgacaggcg cgcagccagt tgttcgtcgg tcgtcgccag
     1021 catggcgatc gcagccagtg ccgcgttcgc cgcgccggcc tcgccgatcg caaaggttga
     1081 aacgggtatg cccttgggca tctgcacgat ggacaacagc gaatcctcgc cgcgcaggta
     1141 tttggacggc accggcacgc cgagcaccgg cacgatggtc ttggccgcga tcatacccgg
     1201 caggtgggcc gcgccgccgg cgcccgcgat gattgcgcgc aggccgcggg cacgcgccga
     1261 ttcggcgtag gcgaacatct cgtctggcat ccggtgggcg gacacgacct tcgcctcgtg
     1321 gggcacgccg aactccttca gcagcgagac cgcatgctgc atcacatccc agtcggagga
     1381 cgaccccatc acgacgccga ccaagggctt gtcactttgt gccatgtcaa ttcacttgtc
     1441 agagaaaatc attgcggatt ggagcggtgc cggccgaggg cgtgtcatcc cgaacgcagt
     1501 gaggtatctc gagcgcgtgc cggaccgtga cgtacagcgc cagaccttgg catgctcgct
     1561 gcagaccgca cacattccca ccgccgtctg gatcagtcct ccagcgcgcg accggtcagg
     1621 cgctgcagcg cctccaggta tttcacgcgc gtcttctcga tcacgtcggg cggcagcgcc
     1681 ggcgccggcg gtgccttgtc ccagtgcttg aggctttcca ggtagtcgcg cacgaactgc
     1741 ttgtcgaagg acggcggcga gctgcccggc gcatacgaat cggccggcca gaagcgtgag
     1801 gaatcggcgg tcagcacctc gtccatcaga tgcagcacgc cattttcgtc gagaccgaat
     1861 tcgaacttgg tatcggcaat gatgatgccg cgcgtagccg catagtcggc cgcggtcttg
     1921 tagagctgga tgctgatgtc gcggatcttt gcggcgagct ccttgccgat acggttctcc
     1981 atctcggcat agctgatgtt ctcgtcgtgc tcgccgatgt cggccttcgc ggccggcgtg
     2041 aagatcggct cgggcagctt gtcggcctgg cgcagtccgg gaggcagcgc gatcccgcag
     2101 accgcgccgc cggcctggta gtccttccag ccggaaccga tcagatagcc gcgcaccacg
     2161 gcttcgacca ggatcggctt caggcgcttg gccacgaccg cccggccgcg cacctgctcg
     2221 acctcgtcgc cggcgacgac cgattcgggt gcagtcccgg tcaggtgatt cggcacgatg
     2281 ccggccagct tctcgaacca gaagttcgac atctggttca gcaccttgcc cttgcccgga
     2341 atcggctcgt tcatgaccac gtcgaacgcc gacaggcggt cggtcgtcac gatcaggatc
     2401 ttgtcattgc cgaccgcata gttgtcgcgg accttgccgc gaccgagcaa cgggagggag
     2461 gtgatggtgg attggtagag gtggtccact tggtttccaa tcaatacgag tccggttctt
     2521 gagcgcacgg caggtgccgt gtcatccggg ctggcaccgg tgcttgcagg caggctctcc
     2581 gagctgtcgt tcccctgggc gggccgcgcc tggggagaca ttgcgagggt ggccgcaacg
     2641 atgaacaccg ttcggacaac agccagggcc ccgaataaaa agccaaaccg gcgggtgttt
     2701 ggacacccgc cggccggttt gctgcaatcc cgtgatttta ctgcacttta ctggacgatc
     2761 tgcgccagtt cgcccttctt gtacttctcg gccatctttt ccagcggaat cggcttgatc
     2821 ttgccggcct ggccttcgca accgaaggcg atgaagcgcg atttgcagat gcccttggcc
     2881 gcttcgcgcg ccggcttcag gtattcgcgc gggtcgaact tggacgggtt ctcggcaaag
     2941 aagcggcgga tcgcgccggt catcgcgagg cggatgtcgg tgtcgatgtt gatcttgcgg
     3001 acaccatgct tgatgccttc ctggatctct tccaccggca cgccgtaggt ttccttcatg
     3061 tcgccgccga actggcggat ctcggccagc agttcctgcg gcaccgacga agagccgtgc
     3121 atcaccaggt gggtattcgg aatgcgcgcg tggatctcct tgatgcgctg gatcgccagg
     3181 atgtcgccgg tcggcttgcg cgagaacttg taggcgccgt gcgaggtgcc gattgcaatg
     3241 gccagcgcat cgcactgggt ctgcttgacg aagtcggcag cctggttgac gtcggtcagc
     3301 agctgctcac gggtcattgt cccttccgcg ccatggccgt cttccttgtc gcccttcatg
     3361 gtttccagcg agccgagcac accgagttca gcttcgaccg tcacgcc
//
LOCUS       DATLGF010000270         5514 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_180742, whole genome shotgun sequence.
ACCESSION   DATLGF010000270 DATLGF010000000
VERSION     DATLGF010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180742"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VM406_08240"
     CDS             complement(<1..556)
                     /locus_tag="VM406_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HVL75992.1"
                     /translation="MTGPRYHGFDTLSLHAGAAPDPTTGARATPIYFTTSYVFKDSDH
                     AASLFNMERAGHVYSRISNPTNAVLEERIAALEGGVAGLAVASGQAAMHLGLATIAGA
                     GSHVVASRALYGGSQNLLAYTMKRFGIETTFVDPRDIDAWRAAIRPNTKVLFGETLGN
                     PGLDVLDIPRVAELAHAHHLPLMVD"
     gene            702..1151
                     /locus_tag="VM406_08245"
     CDS             702..1151
                     /locus_tag="VM406_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVL75993.1"
                     /translation="MKVSEILKVKGDILYTISPDKPLAEAVEIMAEKDIGSLVVMEFG
                     ELVGMLTFREVMQALRDNKGSVGSTTVRKHMDDGPITITPDTEVNEVRRLMLERHARY
                     VPIVSARTLLGVISFYDVAKAVFEAQSFENRMLKAYIRDWPSPAREE"
     gene            complement(1174..4452)
                     /locus_tag="VM406_08250"
     CDS             complement(1174..4452)
                     /locus_tag="VM406_08250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVL75994.1"
                     /translation="MARFAKRRTLTLRRAIVLVVVLGLVLPALLVSGYTWFMRYHQGL
                     RAQTQELLQQNADILANGMQEPLWNINQESGRALLEAMMSRNEDIVRIEVRDNQLGLF
                     AVGEQPERRSGFTAATEKPVVYRGSTIGSVRIEVGSARLRQVMIDNLAGSMIAVLAQI
                     FLSVALIVILLERRLVRPLERLHAGAERLALRRLDEPFTWRRLDEIGMLSRRMEETRI
                     SLRRLFEELDRKNRELEQDIDKRKRIEQELHERETRFRVLVEQSPIAIIEWDRHHQVI
                     EWNAAAERIFGYTREQAIGRHASFLLPNASRETANAVLTRIGNSRTEARNVAHNRRAD
                     GSIITCQWSHTRIAEGSRADRLLSIAEDITEKRRSEEALSLSEAKFAGAFQCNPDPIS
                     IARLSDWLMIDVNQTFEQVTGYSREEVMGRTAIELELWHQTDQRKALVRQLMVSDVLR
                     NFEWDMRTRSGSVRKCLMNATLFSAGSERYILAVMRDITDQRLLEEQKTEADQALLRL
                     AQGTRDIAGEAFFESLVADLASALRTQCAYIGLRVPHAQGNIRTLAAHSRGMPFSNFE
                     YGVSEGPCSETLAGDICIRASNLMEKYPRARWATDTKWQSYAGAPLRDTAGNTIGVLA
                     VLHPEPLGNPDLVRSLLRVFSERASAELERKRAEEELRSSERRFLTFFQFSPVAMYVT
                     HLRENYPIRDANEAFEQLFLHPRSSLVGRSSAEVGLYNDLDDRAALIDELTAAGATRS
                     QREIWMRRADGSRVLAQVSGHSFALAGEKLAILACIDITDKRRIENEVRELNANLERR
                     VVERTEELQQTNRELAATLETLHMAQEELVRSEKLAALGALVAGIAHELNTPIGNSLM
                     VASTLADQTREITARFHASGLKRSDLETYLDDADTAGEIMVRNLQRAANLVTGFKQVA
                     VDQTSSHRRRFSVAEVVSEVMLTLWPTLRKTGFTFTQNVPHSMVMDSYPGPLGQVITN
                     LVNNAVLHGFDGRQSGAVTISASMAAEGAIELVIADDGVGIPSAHLNRIFDPFFTTKL
                     GAGGSGLGLNIVHNIVTGVLGGRLRVQSEPDKGSSFILILPVAAPARGDEETMPLGME
                     RTGV"
     gene            complement(4465..>5514)
                     /locus_tag="VM406_08255"
     CDS             complement(4465..>5514)
                     /locus_tag="VM406_08255"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HVL75995.1"
                     /translation="TVGRVGGDTFGVLGDAALVNPSILLALFDEPFQIDGQEVQVTAT
                     AGLVRLTDYEGSSSDALKDADIALKRAKIQQRAGHFYFSRSMGADVRERVRMMHALRN
                     ACEQNELFLVYQPQIDLPTRRPIGAEALLRWKTSDGSFISPDRFIPIAEYSGLIIDIG
                     ERVMREACRELMRLRGLGFTDFTMSINVSQVQFRHTRFLETLRAVLAETGAPPSRIEL
                     EITESIAMEEPDNLIRLLGQIKQTGVNIAIDDFGTGFSSLSYLQRLQADRLKIDRSFV
                     TEITQSARGSSIAEMVIQLGRNLGIAVVAEGVEDERQAGILDRLGCPYAQGYLFARPM
                     TAEQLTAWLGKNGAR"
BASE COUNT          915 a         1749 c         1791 g         1059 t
ORIGIN      
        1 cgtccaccat cagcggcaga tggtgcgcgt gggcgagctc ggccacacgc ggaatgtcga
       61 gcacgtcgag gccgggattg ccgagggtct ccccgaacag caccttggtg ttgggacgaa
      121 tcgctgctcg ccaggcatcg atgtcgcgcg gatcgacgaa ggtggtctcg atgccgaaac
      181 gcttcatcgt gtaggcgagc agattctgcg agccgccgta cagcgcgcgc gaggcgacga
      241 cgtgcgagcc cgctccggcg atggtcgcca gacctaggtg catcgccgcc tgtccgcttg
      301 ccaccgcgag tccggcgacc cctccttcga gcgcggcgat gcgttcttcc agcaccgcat
      361 tggtcgggtt cgagatgcgc gaatacacat gtcccgcgcg ctccatgttg aacagcgaag
      421 cggcgtggtc ggagtccttg aagacgtacg aggtcgtgaa gtagatcggg gtggcgcgcg
      481 cgcctgtggt cgggtcgggc gctgcgcctg cgtgcagcga tagcgtgtca aagccgtgat
      541 agcgtgggcc ggtcatggca ggtctccgtg tcttgtcatt cgaatgcccg ccatcgtagc
      601 accggctgcc gtttctcgtt gtcatgccgg cgacaggcgc gctggcattt tcaacgcgct
      661 tgcgctacat tcagatgcaa catcgacaag caggcgcaga catgaaagtt tccgaaatcc
      721 tcaaggtcaa gggcgacatt ctctacacca tttctccgga caagccgctg gccgaagccg
      781 tggaaatcat ggccgagaag gatatcggct cgctggtggt gatggaattc ggcgaattgg
      841 tcggcatgct gaccttccgc gaggtcatgc aggcgctgcg cgacaacaag ggcagcgtgg
      901 gctcgacgac cgtgcgcaag cacatggacg acggcccgat caccatcacc ccggataccg
      961 aggtcaatga ggtgcgtcgc ctgatgttgg aacggcacgc gcgctacgtg ccgatcgtca
     1021 gcgccaggac gctgctcggc gtcatctcct tctacgacgt ggccaaggcc gtgttcgagg
     1081 cgcaaagctt cgagaaccgg atgctcaagg cctatatccg cgactggccg tcacccgcgc
     1141 gcgaggaata ggcgcgcccc gttcaggtcc ctctcagacg cctgtccgtt ccattccaag
     1201 cggcattgtc tcttcgtcgc ctcgagccgg cgcggccacc gggaggatga ggataaagga
     1261 actgcctttg tccggttcgc tctggacccg caaccgtcca cccagaacgc cggtcacgat
     1321 gttgtggacg atgttcaggc ccaggccgga gccgccggca cccagtttgg tggtgaagaa
     1381 gggatcgaag atccggttca gatgcgccga agggatgcct acgccatcgt cggcgatgac
     1441 gagttcaatc gcgccttcgg ctgccatgct cgccgatatc gtcaccgcgc cgctctgcct
     1501 gccgtcgaaa ccgtgcagca cagcattatt cacgagattg gtgatcacct ggcccagcgg
     1561 tcccggatag ctgtccatca ccatgctgtg gggaacattc tgggtaaagg tgaaaccggt
     1621 cttccgtagc gtcggccaca gtgtcagcat gacctcggac accacctcgg ccaccgaaaa
     1681 ccggcgcctg tgcgagctgg tctggtcgac cgccacctgc ttgaatccgg tgaccaggtt
     1741 ggccgcgcgc tgcaggttgc gcaccatgat ctcgcccgcc gtgtcggcat cgtcgaggta
     1801 ggtttccagg tccgagcgct tgagcccgct tgcgtggaag cgggctgtga tttcgcgcgt
     1861 ctgatcggca agtgtgcttg caaccatcag gctgttgccg atcggcgtgt tcagctcatg
     1921 cgcgatcccg gccaccagcg cgccaagcgc cgcaagcttc tcgctgcgta ccagttcttc
     1981 ctgcgccatg tgcagggttt ccagcgtggc ggccagttcc cggttcgtct gttgcagctc
     2041 ctcggtccgc tcgaccacgc gccgctccag gttcgcgttg agttcacgca cttcgttctc
     2101 gatgcgccgc ttgtcggtga tgtcgatgca agcaaggatg gcgagctttt cgcccgccag
     2161 cgcgaacgaa tgtccggaca cctgcgccag cacccggctt ccgtccgccc ggcgcatcca
     2221 gatctcgcgc tggctgcgcg tcgccccggc ggccgtcagt tcatcgatga gggccgcacg
     2281 gtcgtccagg tcgttgtaca ggccgacttc agcggaactg cggccgacca ggctgctgcg
     2341 cggatggagg aagagctgct cgaatgcttc gttggcgtcg cggatcggat agttctcgcg
     2401 caggtgggtg acatacatcg ccaccgggga gaactggaag aaggtgagga agcggcgctc
     2461 gctgctgcgc aattcttcct cggcgcgctt gcgttcgagt tcggccgagg cgcgctcgct
     2521 gaatacgcgc agcagcgacc tgaccaggtc ggggttgccc agcggctcgg ggtgaagtac
     2581 cgccagcacg ccgatcgtat tgcccgccgt gtcgcgcagt ggtgcgcccg cgtagctttg
     2641 ccacttcgtg tctgtggccc agcgcgcccg tgggtacttc tccatcaggt tggaggcacg
     2701 aatgcagatg tcgcccgcca gcgtttctga gcacggccct tcgctaaccc cgtactcgaa
     2761 attggagaag ggcatgccgc ggctatgcgc ggccagcgtg cggatgttgc cctgcgcgtg
     2821 cgggacacgc aggccgatgt acgcgcactg cgtgcgcagc gccgaggcga ggtcggcgac
     2881 cagcgattcg aagaaggcct cgccggcgat gtcgcgcgtt ccctgggcca ggcgcagcag
     2941 cgcctggtcc gcctccgtct tttgctcctc cagcaggcgc tggtcggtga tgtcgcgcat
     3001 gacggccaga atgtagcgct cgctcccggc cgagaacagg gtcgcattca tcagacactt
     3061 gcgcacgctg ccgcttctgg tccgcatgtc ccactcgaag ttgcgcagga catccgaaac
     3121 catcagctgg cggaccagcg ccttgcgttg atccgtctga tgccaaagct ccagctcgat
     3181 cgcggtcctg cccattactt cttcgcggct gtagccggtg acttgctcga atgtctggtt
     3241 gacgtcgatc atcagccagt cgctcagacg ggcaattgaa atcggatcag gattgcattg
     3301 gaaggcgccg gcaaattttg cttcggacag actgagcgct tcttccgaac ggcgcttttc
     3361 cgtgatgtcc tcggcgatcg acagcaggcg gtcggcgcgg ctgccctcgg caatgcgcgt
     3421 atggctccac tggcaggtga tgatgctgcc gtcggcgcgc cggttgtgtg ccacgttgcg
     3481 ggcttcggtg cgagagttgc cgatgcgcgt gaggacggcg ttcgcggttt ccctgctcgc
     3541 attcggaagc aggaagctcg cgtgacgccc aatggcttgc tcgcgcgtgt agccgaagat
     3601 gcgctcggcg gcggcgttcc attcgatgac ctggtggtgc cggtcccatt cgatgatcgc
     3661 gatcggactt tgctcaacca gcacgcgaaa gcgcgtctct cgctcgtgca gctcctgctc
     3721 gatgcgcttg cgcttgtcga tgtcctgctc cagctcgcga ttcttgcggt cgagttcctc
     3781 gaacagcctg cgcagactga tccgtgtctc ctccatgcgc cggctcagca tgccgatctc
     3841 gtcgaggcgc cgccaggtga acggctcatc gagcctgcgc aaggcgagac gctcggcgcc
     3901 ggcatgcagg cgttccagcg ggcgaacgag ccggcgttcg agcaggatca cgatcagggc
     3961 gacggacaga aatatctgcg ccagcaccgc gatcatcgag ccggcgaggt tgtcgatcat
     4021 gacctggcgc agtcgcgcgc tgcccacctc gattctcacc gagccgatcg tgctgccgcg
     4081 atagacgacc ggcttctcgg tggcggcggt aaagccgctg cgccgctccg gctgttcccc
     4141 taccgcaaac aggccgagct ggttgtcccg cacttcgatg cgcacgatgt cctcgttgcg
     4201 cgacatcatg gcctcgagca gcgcgcgtcc gctttcctgg ttgatgttcc atagcggttc
     4261 ctgcatgccg ttggcaagaa tgtcggcgtt ttgctgcagc agctcctggg tctgggcgcg
     4321 caagccctgg tggtagcgca tgaaccaggt atagccgctg acgagcaggg ccggcagaac
     4381 gaggccgagc acaacgacca ggacgattgc gcgtcgcagc gtcagcgtgc gtcttttggc
     4441 gaaccttgcc atgccgcccc gcgctcagcg cgcgccgttc tttccgagcc aggcagtcag
     4501 ctgttcagcc gtcatagggc gtgcgaacag gtagccctgc gcatacggac agccgaggcg
     4561 atccagtatg ccggcctggc gttcgtcctc cacgccttcg gccacgacgg caatccccag
     4621 gttgcgcccg agctggatca ccatttcggc gatgctgctg ccgcgcgcgg actgggtgat
     4681 ctcggtcacg aagctgcggt cgatcttcag gcggtcggcc tgcagccgct gcaagtagct
     4741 gagcgaagag aatccggtgc cgaaatcgtc gattgcaatg ttcacgccgg tctgcttgat
     4801 ctggccgagc aggcgtatca gattgtccgg ctcctccatc gcgatcgatt cggtgatctc
     4861 cagttcaatg cgcgacggcg gggcaccggt ctcggccagc accgcgcgca gcgtttcgag
     4921 gaagcgcgtg tggcggaact gcacctgcga cacattgatc gacatcgtga aatcggtgaa
     4981 tccgagcccg cgcaggcgca tcagttcgcg gcaggcctcg cgcatcacgc gctcgccaat
     5041 gtcgatgatc agtcccgagt actcggcaat cgggatgaat cgatcaggcg agatgaagct
     5101 gccgtcgctg gtcttccagc gcagcagggc ttcggcaccg atcggcctgc gcgtgggcag
     5161 atcgatctgc ggctggtaga cgaggaagag ttcgttctgt tcgcaggcat tgcgcaatgc
     5221 gtgcatcatg cgcacgcgct cgcgcacgtc ggcgcccatg ctgcgcgaga agtagaagtg
     5281 accggcgcgc tgctggatct tggcgcgctt tagcgcgatg tcggcatcct tcagcgcgtc
     5341 ggaggaactg ccctcatagt cggtcagccg gacaagaccc gcggtggcgg tcacctggac
     5401 ttcctgcccg tcgatctgga atggctcgtc gaacagtgcc agcaggatgg acggattgac
     5461 gagggcggcg tcgccgagaa cgccgaaggt gtcgccgccg acccgaccca ccgt
//
LOCUS       DATLGF010000271         5038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_43916,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000271 DATLGF010000000
VERSION     DATLGF010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43916"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VM406_08260"
     CDS             complement(<1..927)
                     /locus_tag="VM406_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HVL75996.1"
                     /translation="MMDTLNGMLLGFGTALAPGNLMYAFLGVVLGNVVGVLPGIGPLA
                     AISMLLPITFSIEPAAALMMLAGIYYGAQYGGATTSIMLNLPGVASHAVTCLDGHPMA
                     KKGRAGAALLLAMLASFFGATIGILIMMFFSPIISETALKFGPAEYFSMMVLGLLAAT
                     TLSQGSAVKGVVMVVLGIVLGVVGTDVNTGTARFTFGIPHLYDGISLVALAMGLFGIA
                     DVLRSVNRIGKANVQGKGVGWRNLKLTKEETRRSIKPMARGTGIGAFFGVLPGTGSTI
                     ASFMSYALEKKVSKTPQAFGKGAVEGVVGPEAS"
     gene            complement(931..1395)
                     /locus_tag="VM406_08265"
     CDS             complement(931..1395)
                     /locus_tag="VM406_08265"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HVL75997.1"
                     /translation="MNLRQFIKRWGASVLVTGIGIATVVGGLGHHVGTAARMGPGYFP
                     VALGILLIGLGIIVGLITDRDALKDEQVEEHVVDLRGWGCIIAGIILFMILGEYAGFI
                     PATFVLVFVAALGDRENTLKSAAILAAGVTVFGVLVFTQLLELNIPLFKFGS"
     gene            complement(1911..3767)
                     /locus_tag="VM406_08270"
     CDS             complement(1911..3767)
                     /locus_tag="VM406_08270"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HVL75998.1"
                     /translation="MNQPVMGGIAPLNAPSYVKQRKLLNWVAEIAALTKPERIYWCDG
                     SQEEYDRLCAEMVAAGTLKKLNEAKRPNSYLALSDPSDVARVEDRTFICSQKQEDAGP
                     TNNWVAPAEMRVTLNGLFDGCMRGRTMYVVPFSMGPLGSPIAHIGVELSDSPYVAVNM
                     RIMTRMGRAVYDVLGEDGDFVPCVHTVGKPLAAGEQDVPWPCNPTKYIVHYPETREIW
                     SFGSGYGGNALLGKKCFALRIASTMGRDQGWLAEHMLILGVESPEGKKHYVAAAFPSA
                     CGKTNFAMLIPPKAFNGWKVTTIGDDIAWIKPGADGRLYAINPEAGYFGVAPGTNSSS
                     NANCMASLSANAIFTNVALTDDGDVWWEGMSKEPPAHLIDWQGKDWTPQIAKETGRKA
                     AHPNARFTVAATQNPVIDPAWDDPAGVPISAFIFGGRRSTTVPLVTEARNWVEGVYMA
                     ATMGSETTAAAAGQQGVVRRDPFAMLPFMGYNMSDYFQHWLDMGKKIAASPAELPKIY
                     CVNWFRTDENGKFVWPGFGDNMRVLKWMLDRIEGNAGGVEHIFGVTPRYEDIKWDGLE
                     FSREQFDKITSIDKSAWEAELKLHAELFEKLKYHLPSELESTKAMLEKRLAA"
     gene            4158..>5038
                     /locus_tag="VM406_08275"
     CDS             4158..>5038
                     /locus_tag="VM406_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HVL75999.1"
                     /translation="MKIAILDDYQDCVRHLDCFRLLDGHDVKVFTNSARGLMQLALRL
                     AEFEVLVLNRERTAITAALLQRLPKLRLLAQTGRVGAHIDLAAAHARGVAVAEGVGDP
                     TAPAELTWALIMAAMRKVPQYATNLREGRWQTASINPELNTLGRVLKGRTLGIWSYGR
                     IGRLVAGYGRAFGMRVLVWGSETSRAAAVADGFEAAESKEALFGQSDVLSLHLRLNEA
                     TRALVGPDDLARMRRDALIVNTSRAELIAPGALQAALHRGRPGYAALDVFESEPLPAD
                     DALLKIPTVLATPHIGYV"
BASE COUNT          871 a         1615 c         1672 g          880 t
ORIGIN      
        1 cgacgcctcc ggcccgacca ccccttcgac cgcgcccttg ccgaacgcct gcggcgtctt
       61 cgacaccttc ttctcgagcg catacgacat gaacgaggcg atcgtcgagc cggtgcccgg
      121 cagcacgccg aagaacgcgc cgataccggt gccgcgcgcc atcggcttga tcgaacggcg
      181 cgtctcttcc ttcgtcagct tcaggttgcg ccagcccacg cccttgccct gcacattggc
      241 cttgccgatg cggttcacgc tgcgcagcac gtcggcgatg ccgaacaggc ccatcgccag
      301 cgcgaccagg ctgatgccgt catacaggtg cgggatgccg aaggtgaagc gggcggtacc
      361 ggtgttcacg tcggtgccga ccacgcccag cacgatgccc agcaccacca tcaccacgcc
      421 cttgactgcc gaaccctggg acagcgtggt cgcggccaac aggcccagca ccatcatcga
      481 gaagtactcg gccgggccga acttcagcgc ggtctcggaa atgatcggcg agaagaacat
      541 catgatcagg atgccgatcg tcgcgccgaa gaacgaggcc agcatcgcca gcagcagcgc
      601 tgcaccggcg cggcccttct tcgccatcgg gtggccgtcg agacaggtca ccgcgtgcga
      661 agccacgccg ggcaggttca gcatgatcga ggtggtcgcg ccgccgtact gcgcgccgta
      721 gtagataccg gccagcatca tcagcgcggc cgccggttcg atcgagaagg tgatcggcag
      781 cagcatcgaa atcgccgcga gcgggccgat gcccggcagc acgccgacca cgttgcccaa
      841 aacgacgccg aggaacgcat acatcaggtt gccgggtgcg agcgcggtgc cgaacccgag
      901 cagcatgcca ttcagtgtgt ccatcatggt ttagctcccg aacttgaaga gagggatgtt
      961 gagctcgagg agctgggtga acaccagcac gccgaagacg gtgacgccgg ccgcgaggat
     1021 cgcggcggat ttcagcgtgt tctcgcggtc gccgagcgcg gcgacgaaca ccagcacgaa
     1081 ggtggcgggg atgaagccgg cgtactcgcc gaggatcatg aacaggatga tgccggcgat
     1141 gatgcagccc cagccgcgca ggtcgacgac atgctcctcg acctgctcat ccttgagcgc
     1201 gtcgcggtcc gtgatcaggc cgacgatgat gccgaggccg atcagaagaa tgccgagggc
     1261 gaccgggaaa tagcccggcc ccatgcgggc cgcggtaccc acgtgatggc caaggccgcc
     1321 gacgacggtg gcaatgccta tgcccgtcac cagcacgctg gcgccccagc gtttgatgaa
     1381 ttgtctaagg ttcatgctgt ctccgaatga atagcaaggg gaacgcgcag cgcgttgaat
     1441 cgcatggcga ggtgaggaag gatgggcctc gtccgccagc cttccgccgg gggcagaaag
     1501 gcgcagcgcg attattgcga ggcgggaggg gggcgacaat tgtggatagc cgcagattcg
     1561 ccggcgcggc cggagatgcc gggcgggaac cgggcggata cggcgctgtg ccaggaatga
     1621 cgggcttttc tcgcggtggt cgccgtggtt cagcgtttgg gcaactgttc ttctccacgg
     1681 cgaacacggc gatacacggc gcaaacccca acttcctgtc gtcccgagca cagcgcggga
     1741 cctcgtcgcc tcgcacaccg gagattcctc gctgcgctcg gaacgacagt ttgttcgccg
     1801 tgcccgccgc gatccaggtt ttttcgggaa ggcacccgcc cggccatgaa aaaaggcgcg
     1861 gcggattgct ccgccgcgcc ttcctgaaag gactggatcg aacgtggcga tcaggccgcc
     1921 aggcgcttct ccagcatcgc cttggtcgat tccagctccg acggcaggtg gtacttcagc
     1981 ttctcgaaca gctcggcgtg cagcttcagt tccgcctccc aggcgctctt gtcgatcgag
     2041 gtgatcttgt cgaactgctc acgggagaac tccagcccgt cccacttgat gtcctcgtag
     2101 cgcggcgtca cgccgaagat gtgctcgacg ccgccggcat tgccttcgat gcggtcgagc
     2161 atccacttca agacgcgcat gttgtcaccg aagcccggcc agacgaactt tccgttttcg
     2221 tcggtgcgga accagttcac gcagtagatc ttcggcagct cggccggcga cgcggcgatc
     2281 ttcttgccca tgtcgagcca gtgctggaag tagtcgctca tgttgtagcc catgaacggc
     2341 agcatcgcga acggatcgcg gcgcaccacg ccctgctggc cggcggccgc ggcggtggtt
     2401 tccgaaccca tggtcgcggc catgtacacg ccttcgaccc agttgcgcgc ctcggtcacc
     2461 agcggcaccg tggtcgagcg gcggccgccg aagatgaagg ccgagatcgg cacgccggcc
     2521 gggtcgtccc aggccggatc gatcaccggg ttctgggttg cggccaccgt gaagcgcgcg
     2581 ttcggatgcg cggccttgcg gccggtttcc ttggcgatct gcggcgtcca gtccttgccc
     2641 tgccagtcga tcaggtgcgc cggcggctcc ttgctcatgc cttcccacca gacgtcgccg
     2701 tcatcggtca gcgcgacgtt cgtgaagatc gcgttggccg acagcgaggc catgcagttg
     2761 gcgttggagc tgctgttggt gccgggcgcc acgccgaagt agccggcttc cgggttgatc
     2821 gcatacaggc ggccgtcggc gcccggcttg atccaggcga tgtcgtcgcc gatggtggtc
     2881 accttccagc cgttgaaggc cttgggcggg atcagcatcg cgaagttggt cttgccgcag
     2941 gccgacggga aggcggcggc cacgtaatgc ttcttgccct cgggcgactc gacgccgagg
     3001 atcagcatgt gttcagccag ccagccctgg tcgcggccca tggtggaggc gatgcgcagt
     3061 gcgaagcatt tcttgccgag cagcgcgttg ccgccgtagc cggagccgaa cgaccagatc
     3121 tcgcgggttt ccgggtagtg gacgatgtac ttggtcgggt tgcacggcca cggcacgtcc
     3181 tgctctccgg cggcgagcgg cttgccgacc gtgtgcacgc aggggacgaa atcgccgtct
     3241 tcgccgagca cgtcgtagac cgcacgtccc atgcgcgtca tgatgcgcat gttgaccgcc
     3301 acatacggcg aatcggacag ctcgacgccg atatgggcga tcggcgaacc cagcgggccc
     3361 atcgagaacg gcaccacgta catcgtgcgg ccgcgcatgc agccgtcgaa caggccgttc
     3421 aacgtgacgc gcatttcggc cggggcgacc cagttgttgg tcgggccggc gtcttcctgt
     3481 ttctgcgagc agatgaaggt gcggtcttcg acgcgggcga catcggacgg atcggacagc
     3541 gcgagatagc tgttcgggcg cttggcttcg ttgagcttct tcagcgtgcc ggcggcaacc
     3601 atctcggcgc acaggcggtc gtactcttcc tgcgacccgt cgcaccagta gatgcgctcg
     3661 ggcttcgtca gggccgcgat ctcggcgacc cagttgagca gcttgcgctg tttaacgtag
     3721 gacggtgcat tcaatggtgc aatgcctccc ataaccggtt ggttcataca aactcccttt
     3781 aaagcaaata aagaattcgg tctggttccg gggcggcagg atcgtcgtgg acagctcgtg
     3841 gctggtgcag cgcgggaagg gcttgcgtac ggcggtcatg tcggcggcgc cgggcgcatg
     3901 cggcgtccgg gcgcgtccgg cccgaaaatg aaataattgc ccgggccggt ggagtggggt
     3961 catggagtcc tcctgttgtg cgacgagcgc tcattacccc ttcaataagg tggcgattgt
     4021 acacgccggt cccctgcccc ctcaatgagt tacaacaaat ttgtaggaat aattgtcgac
     4081 taatgtagta ggcaatacgg gggcagcggc tttaccgtcg caattttcca gttcagcctt
     4141 gcaacgtcct tccagcaatg aaaatcgcga tactcgacga ttaccaggat tgcgtgcgac
     4201 atctcgactg tttccgcctt ttggacggac atgatgtcaa ggtgttcacg aacagcgcac
     4261 gcggtttgat gcagcttgcg ctgcgccttg cggaattcga ggtgctggtg ctcaaccgcg
     4321 agcgcaccgc gatcactgcg gcgcttttgc aacgtcttcc gaagctcagg ctgctcgccc
     4381 agaccgggcg cgtgggcgcg cacatcgacc tggctgcggc gcacgcgcgc ggtgtggcgg
     4441 tggccgaagg cgtcggcgat ccgaccgcac cggccgaact gacttgggcg ctgatcatgg
     4501 cggccatgcg caaggtgccg cagtacgcga ccaacctgcg cgagggccgc tggcagacgg
     4561 cgtcgatcaa tcccgaactc aataccctgg gccgggtgct caagggacgc accctcggca
     4621 tctggagcta cggccgcatc ggccggctcg tggccggcta cggacgggcg ttcggcatgc
     4681 gcgtgctggt gtggggcagc gaaacgagcc gcgcggcagc ggtggccgac ggtttcgaag
     4741 cggccgaatc gaaagaggcg ctgttcgggc agtccgatgt gctgagcctg catctgcgcc
     4801 tgaacgaggc cacgcgcgct ctggtcggcc ctgacgacct ggcgcgcatg cggcgggatg
     4861 cgctcatcgt caacaccagc cgcgccgagc tgatcgcgcc cggtgcgctg caagcggcct
     4921 tgcaccgcgg ccgccccgga tacgcggcgc tcgatgtgtt cgaaagcgag ccgctgccgg
     4981 ccgacgatgc gctgctgaag atccccaccg tgctcgccac gccgcacatc ggctatgt
//
LOCUS       DATLGF010000272         3307 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_138181, whole genome shotgun sequence.
ACCESSION   DATLGF010000272 DATLGF010000000
VERSION     DATLGF010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138181"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VM406_08280"
     CDS             <1..530
                     /locus_tag="VM406_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HVL76000.1"
                     /translation="FAFEKFAGFDADEWLAIKGSFKPNPLPTAPTLFGSDISGDMIVM
                     TRNNLRNAGIQFEVPLKQVEAQEIRPPVETPGIILTNPPYGERIGVRGDRSQAPDELA
                     AAFYSAFSTTLKQRFAGWQVFLFTADLGLPKMLRLKESRKTPFYNGALECRLFRFDMV
                     AGFNRREEARPKPAP"
     gene            862..1242
                     /locus_tag="VM406_08285"
     CDS             862..1242
                     /locus_tag="VM406_08285"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HVL76001.1"
                     /translation="MEFSPVILAHIYTALGAVVFGGATLALRKGTRLHRVLGRIWVGL
                     MVSTALISFGIKTSGHFSAIHILSVVTLAGVSAAVFAAARGRIRAHRAGMTATYAGLV
                     IAGAFTLLPGRLLGDLVWGALGIA"
     gene            1253..1528
                     /locus_tag="VM406_08290"
     CDS             1253..1528
                     /locus_tag="VM406_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HVL76002.1"
                     /translation="METTQRYRLARRHVERRIGFFIHLGVYLTVNAGLVALNLFTVPT
                     RPWSVWPLLGWGVGLLFHALAVFLHAPGARWKERMIANEMNKIRSPE"
     gene            1525..2601
                     /locus_tag="VM406_08295"
     CDS             1525..2601
                     /locus_tag="VM406_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HVL76003.1"
                     /translation="MNLAPPAPAVAYPRLRRFAIDVAIVSVFNVAIALFITYVLRIHD
                     SLTDNIVISMCIGTLAVTFIDGTRLLLWPHRAPPLVPFLLLLAVSIPAAQLIGSRIAG
                     ALTGIPSLEIAGVRSGNVPGAIVMLVLVCLGITWFFWSRERLEHLKAEAAAEKARAAE
                     IERRALQAQLQLLQAQTEPHMLFNTLANLQGLIAVDPPRAGAMLDQLIQYLRASLSSS
                     RAQAVTLEQEFALLDAYLGLMKVRMDARLSYRLDLPAELAALPVPPMLLQPLVENAIK
                     HGLEPKLDGGALEVKATRRDRELVLSVCDTGLGDACHAHTAGGGTGLANVRERLAALF
                     GGHASLLLQPNQPCGLRATLTIPL"
     gene            2598..>3307
                     /locus_tag="VM406_08300"
     CDS             2598..>3307
                     /locus_tag="VM406_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HVL76004.1"
                     /translation="MNNGPLALIAEDEPILAQALRDALARLWPELRTSMAGNGVAAVE
                     QALALAPDVLFLDIKMPGKSGLEAVGELAEEWHEERPFPLIVFVTAYDDYALEAFEQA
                     AVDYVLKPVADARLARTVARLRERLAAQPDRAGELARAIERLHVLAAPAMGADRLRFI
                     RAAIGNQVRMIAVNEVLALQAGDKYVAVITAQGEALIRTSLKELLPRLDPEQFWQIHR
                     SCVINASAIEAAVRDDAG"
BASE COUNT          524 a         1143 c         1060 g          580 t
ORIGIN      
        1 gcttcgcgtt cgagaaattc gccgggttcg acgcggacga atggctggcc atcaagggca
       61 gcttcaagcc gaacccgctg ccgaccgcac cgaccttgtt cggcagcgac atctccggcg
      121 acatgatcgt catgacgcgc aacaacctgc gcaatgccgg catacagttc gaggtgccgc
      181 tcaagcaggt cgaggcgcag gagatccgcc cgccggtcga aacgcccggc atcatcctca
      241 cgaatccgcc atatggcgaa cgcatcggcg tgcggggcga ccgcagccag gcgccggacg
      301 aacttgccgc tgcgttctac agcgcgttca gcacgacctt gaagcagcgc tttgccggct
      361 ggcaggtctt cctgttcacc gccgatctgg gcctgccgaa aatgctgcgc ctgaaagaat
      421 cgcgcaagac cccgttctac aacggcgcgc tcgaatgccg gctgttccgt ttcgacatgg
      481 tggcgggatt caaccggcgc gaggaagccc ggccgaaacc ggcgccctga tcgaattcct
      541 tttaattcag cgaatactct tgctttgtcg ttccccctag gcggcgcccg cttaggcggg
      601 aacccagttc aaccgcctgt aggaccgcgc acgaccgagc tgggcttccg tctgcgcggg
      661 agcgacataa caacgttgtt cgccaactga atggcgttcg gcgccctgaa cgacggcgga
      721 gtgtcctgcg gttgccgatc cgttcatccc cggaatccgc agtttgctcc ccgcttcccg
      781 caactcgtcg caatccgctc gcgcgccgcg tcccgcccgg cgacactgcc tccactgcaa
      841 gaaaccgcaa tggaggccag catggaattc agtcccgtca tcctcgccca catctacacc
      901 gcgctcggcg cggtcgtctt cggcggtgcg acgctggcgc tgcgcaaggg cacgcgcctg
      961 caccgggtcc tcgggcgcat ctgggtcggc ctgatggtgt cgaccgcgct gatttcgttc
     1021 gggatcaaga ccagcggcca cttttcggcc atccacattc tttccgtggt gacgctcgcg
     1081 ggcgtctccg ccgccgtgtt tgcggcggcg cgcggccgca tacgcgcgca ccgtgccggc
     1141 atgacggcga cctacgccgg ccttgtcatc gccggcgcct tcacgctgct gccgggtcgc
     1201 ctgctgggcg accttgtttg gggcgcgctc ggaatcgcat aaggagcaca cgatggaaac
     1261 cacgcaacgc tatcgcctgg cccgccggca tgtcgaacgc aggatcggct tcttcatcca
     1321 cctgggcgtg tatctgaccg tcaatgccgg cctggtcgcg ctgaacctgt tcacggtccc
     1381 gacgcgaccc tggtcggtgt ggccgctcct tggctgggga gtcgggttac tatttcatgc
     1441 gcttgccgtc ttcctgcatg cgccaggcgc acgctggaag gagcgcatga tcgccaacga
     1501 gatgaacaag atccgcagcc cggaatgaat ctggcccctc ccgctcccgc cgttgcctat
     1561 ccccgcctgc gccgcttcgc gatcgatgtg gcgatcgtct cggtattcaa tgttgcgatc
     1621 gcgctgttca tcacctacgt gctgcgcata cacgattcac tgacggacaa catcgtgatc
     1681 tcgatgtgca tcggcacgct ggccgtcacc ttcatcgacg gcacgcgcct gctgctgtgg
     1741 ccgcatcgcg cgccgccgct tgtgcccttc ctgctgctgc ttgcagtgtc gatccccgct
     1801 gcccagttga tcggcagccg catcgccggc gcgctcaccg gcattccttc gttggagatt
     1861 gccggagttc gcagcggcaa tgtgcctggc gcaatcgtga tgctggtgct ggtctgcctg
     1921 ggcatcacct ggttcttctg gagccgggag cggctcgaac acctgaaggc ggaagcggca
     1981 gcggagaagg cgcgcgcggc cgagatcgag cggcgcgcgc tgcaggcgca gctgcagctt
     2041 ctccaggctc agaccgaacc gcacatgctg ttcaataccc tcgcaaacct gcagggcttg
     2101 atcgcggtcg atccgccgcg cgccggcgcg atgctcgacc agctgatcca gtacctgcgc
     2161 gcctcgctct cgtcctcacg cgcgcaggcg gtgaccctcg agcaggagtt cgcgctgctc
     2221 gacgcctatc tcgggctgat gaaggtccgc atggatgcgc ggctgtccta ccgcctcgac
     2281 cttcccgccg aactggccgc actgccggtg ccgccgatgc tgctgcagcc cctcgttgaa
     2341 aacgcgatca agcatggttt ggagccgaag ctcgatggcg gcgcgctgga ggtcaaggcg
     2401 acgcggcgcg accgggaact ggtgctgagc gtttgcgaca ccggcctggg cgacgcctgc
     2461 cacgcgcata ccgccggcgg cggcacgggt ctcgcgaacg tgcgcgagcg gctggctgcg
     2521 ctgttcggcg gccacgcgag cctcttgctg caaccgaacc agccctgcgg gctgcgtgcg
     2581 acgctcacga ttccgctatg aataacggac cgctggccct gatcgccgaa gacgaaccca
     2641 tccttgcgca ggccctgcgg gacgcgctgg cccgcttgtg gccggagctt cggaccagca
     2701 tggccggcaa tggcgtcgcc gcggtcgaac aagcgctggc actggcgccg gacgtgctgt
     2761 tcctcgacat caagatgccc ggcaagagcg ggctggaagc cgtcggggag ctggccgagg
     2821 aatggcacga ggagcggccg tttccgctga tcgtgttcgt caccgcctac gacgactacg
     2881 cgctcgaggc attcgagcag gcggcggtcg actacgtgct caagccggtg gccgacgcgc
     2941 gccttgcccg cacggtcgcg cgcctgcgcg aacggctggc cgcgcagccc gaccgcgcgg
     3001 gtgagctcgc acgggcgatc gaacggctgc atgtgctggc cgcgccggca atgggcgccg
     3061 atcggctgcg cttcatccgc gccgccatcg gcaaccaggt gcgcatgatt gcggtaaacg
     3121 aggtgctggc cctgcaagcg ggtgacaagt acgtcgccgt catcaccgcc caaggggagg
     3181 cgctgatccg caccagcctg aaggaactgc tgccccggct ggacccggag cagttctggc
     3241 agatccaccg ctcctgcgtc atcaacgcct ccgcgatcga ggcagcggtg cgcgacgacg
     3301 ccggaaa
//
LOCUS       DATLGF010000273        15116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_5833,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000273 DATLGF010000000
VERSION     DATLGF010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 15116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15116
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5833"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VM406_08305"
     CDS             complement(<1..583)
                     /locus_tag="VM406_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVL76005.1"
                     /translation="MNARIDTSARAGGDKRRIVMLGTDFHTKGGISSVVNVYRETGLF
                     ERFPIVYLASHVDGPASQKIRYFATAWVRYMAMLLMGRVAAVHIHVSTDMSFWRKLTF
                     ILPTYAFGVPAILHLHGADFDTWYARGPKFKQWIMRYAFDRAASLLVLSQSWRKWAQS
                     VSRNPSIVSIYNPVRVPTATEYATRDAASLLFLG"
     gene            complement(580..2025)
                     /locus_tag="VM406_08310"
     CDS             complement(580..2025)
                     /locus_tag="VM406_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76006.1"
                     /translation="MNVEDLLHPLLNSYLKSPQWVKNSVGRAYSAVPVSWRRGRNYRR
                     FVEEAKPRPEAQLRDLTTAKLARTLHHALHSATAYRPWRALASELADPVAVLRCLPLI
                     SKEDVKRDVNSFLAHDVDPALRMKMFTGGSTSLPMTLYLHKGVTRAKEYAYIEAFHRR
                     AGMREHDVVATLRGNLEPGAGNGEGMWAYEPIKRQLMLSNEHLSPEHMPQYVEAMRRW
                     KPRFIQAYPSAAWALAQWFRQKPDNDVTGRIEAVMLFSETVPADQLTLLRAVFDCPVL
                     CHYGHSERLLMAATMPDDDRYFFWPQYGYFELVDADGNVIERPGELGEIVGTGFDNDV
                     MPLVRYRTGDMAMLSDRPHPLLPGFPAVERVEGRIQEFIVCEGGRVVGLNALTTARQH
                     PLLEFAHAIQFEQRAIGKLTICLVMPRRLSADELRQIAASMESRIGTGCTVEALQVER
                     IERTARGKALMLRQHLDMRPYFRRIEGAQAA"
     gene            complement(2096..3415)
                     /locus_tag="VM406_08315"
     CDS             complement(2096..3415)
                     /locus_tag="VM406_08315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL76007.1"
                     /translation="MDGAAQVWSERAGSAHLKSGEQSVFAAERQCRPALRHFLSNSSG
                     LFALARPLPAGGASRMPGNGPAPMSGSAHKFPHRFDWRPASGMLTEIDSCSEPSDSHL
                     FASPAYVRAFQSMQAVHDLVAERGDMPAATLLFRVERGRATMLTEFVDFSAEDMEHAA
                     GAVFQRFPDVGVVSMPAVQRRHALERFPARCYTDRADIVIALPDTTEAYTASLSQSTR
                     STVRNRLNRLRREHPSMTVRAEDGWSADPDLISRLVAMSNARLSDTGRHSYNDAGMVH
                     NFHRLAATSRSLLLTIWIDGQLCGGSWNVRAGSGYFGFINTFDQRYSRYSLGMLSAYL
                     SVCEAIRQGARRFHTGWQRYDYKYQLGGAEQPSWRYEIYRSQLHCVRRAPQIVLDVAE
                     DRMRQLKTWMQAPERRNRFLTRAATASVNRVRALAGRHTDRAAAKDR"
     gene            complement(3550..4410)
                     /locus_tag="VM406_08320"
     CDS             complement(3550..4410)
                     /locus_tag="VM406_08320"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL76008.1"
                     /translation="MTQAHAYGEAKAATGSLHAQRYLIEYNGAPVAMVQALVLRAAGI
                     ALGARINRGPLFFDETPSLALREGVMQAIRRYWRIGRHGALLIAPALDDTSENRALLH
                     RLGYRLRKEQGWCSALVDLSPPEEEIQKRFAPVWRNRLRAAQKSGLEIRHCNDVQGLE
                     WMLDRHEQNQRTKRFNGASRALLKALYMAKPGDFRILQAMAGSRAVGGLVLYRFGDRA
                     EYYVGWFGDDGRKLNCGNFLYWHAICEAKRLGHRWFDVGGYYSSDKFGHFKQNLRGTE
                     YRLAGEWLSL"
     gene            complement(4953..5930)
                     /locus_tag="VM406_08325"
     CDS             complement(4953..5930)
                     /locus_tag="VM406_08325"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL76009.1"
                     /translation="MQTTTMFAPSAAAMQSYRMRPVTSAVEWGEYFHALPQAHLMQSY
                     PYGEAKRAAERLHVERYVFEQGRTPVAICQVLERRLAGVRVGARINRGPVFLDSAPSY
                     GVREGVMKLVRMNWRTGRGGPLLIAPALPDSEENRTLLRSLGFRCRRERGWCSSLIDL
                     GLSEEEIRKRLSSSWRNHLKGAMKSGLELRVSQSDGSYQWMVERHQENMRAKGFAGPK
                     PDLLLALHAADPNEVQVLQALHQDRPVAGMILARFGHTVEHYVGWFGPEGRKLQSGNY
                     LYWQAICESKKAGYRWFDVGGYDTNDKFGHFKMNMRGHEYRLAGEWLGL"
     gene            complement(5970..7232)
                     /locus_tag="VM406_08330"
     CDS             complement(5970..7232)
                     /locus_tag="VM406_08330"
                     /inference="COORDINATES: protein motif:HMM:NF026235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HVL76010.1"
                     /translation="MRLLLFLLVLVTAASSFARGGADERLTRRPEPGFPRLLGMQIGE
                     KHYDDPDYQRQMARLDIVILGFYRGWGERPGAMREVVRELRRLNPSILVGQYTILNEA
                     SNDADNTAEQDKQAALRENGWWLTTAAGKPVQWTEIYGAWDINITAWTRKDRNGLRYP
                     QWLALRDHRVYFEPVPEFDIWFFDNVMHAQRIRLADWRGSGRNQSGTDPEVQRAFRRA
                     QAEHWAAARRLSPHRLFIGNTDNDLSYPEFRNRLQGAFLETLMGESWSLYERLGWNAM
                     MTRYHSVFNNLAAPRIVSFNVWGAADDYRLMRFALASCLMNDGYFSYTDQARGYSSVP
                     WFDEYDARLGRAIDPPQRSPWKNGVYRRRFEHGLVLVNPTRETRRVDIGPGYRHLRGE
                     QAPQINSGRAVSSIELAPQDGVLLLSGS"
     gene            complement(7242..8282)
                     /locus_tag="VM406_08335"
     CDS             complement(7242..8282)
                     /locus_tag="VM406_08335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVL76011.1"
                     /translation="MKPHDPRVTVVMSAFNAEATLRSALESLRRQHFDRFEVVVVDDG
                     STDATADIARSWPDERVRLVQNVRHCGLAMARNLGIAEARGEYIALLDAHCCALPQRL
                     AEQVAALDADPELRLVGSHLAVINAHGKLGDIWMRPTSAAETRIQMLFRDALSGVTFR
                     KSAMPEGGFRALPAGVDFDFNARVAAGGKVCNLDRTLTAVRLLPSKPSWEQKVLAENC
                     MRGIMRSQLRRFGVEASERELAVNRHVGGHTLFPTVQLLQDVEAWLLKLCLANWELQR
                     YPDPDFKRLLGYEWYQVCKCASPLGREALRVWRSSPLLKHWQPGVLELARFVTKCLLR
                     HERLEDDMPALA"
     gene            complement(8269..9762)
                     /locus_tag="VM406_08340"
     CDS             complement(8269..9762)
                     /locus_tag="VM406_08340"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HVL76012.1"
                     /translation="MPRSESLRTSAVLLGFAGMIEFALQMAIPILLARYLDEITFAQY
                     RLLWLLAGTALAIAPMFMPQSLFYFLSRARRRRKASYIGNVLLFLTAAGTLSALVASD
                     LNPWLQQAALDLFRDSAGLSAAFLALWVMASMLDVLPTADGRAAWQAGAIIGLAVLRT
                     VLLAAAALFAGDIYWIAVVMLAVASAKLLLLAVYMRRFIARTDSEWDLALLRTQVMYA
                     FPFAIGHTLFLLRVQADQWVVVSMLPTAMYATFSVAAVILPIGTLIRQPVNNALLPRL
                     NKVFGAGDKAQAARLIALSNGASGMLLIPVAGALFACAPELVELVYTRRYIEAAPVMR
                     VYLIGLMITAIAVGHTLSALGHGRFAAINSAVCLALSIVLCVTLTRQFGMAGAAAGSM
                     IALAVGELWGLLVVARTLHSSALRLINWHSLLPAILATAFALLMVEWVTGDRTLAPWN
                     MLLAKAGVYCVAFVPVFLLAGGWRAATELAGFRKDKAVVEGGAHETA"
     gene            complement(9752..10924)
                     /locus_tag="VM406_08345"
     CDS             complement(9752..10924)
                     /locus_tag="VM406_08345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVL76013.1"
                     /translation="MNGRRIHVLLIVNSLCVGGAEKHAVTLLNTLDTSRFRLSFAYLK
                     SEDALLPQIMSERAEGGVFCAGVSRKIDRAAARRLADHLRGEHIDIVLCTNTYSLLYA
                     WLARMASAASGGPRPRIVEVFHSTELGSLEDELQMLFYRPWILACDRLVYVCENQRRY
                     WRRRALRARGDAVIHNGIDIAHFADGYSDREKAAVRAAYGLGSGDYVIGLCATMRPEK
                     AHGDLLRALVRLRDEGVRARALLIGDGPMRAEIEAVIERLGLGGHACITGFASDVRPL
                     IAACDVMAITSHHVETFSIAALEAMALGKCMVMTDIGGAAEQIRHGDNGFLYRRADIA
                     ALCAALHTLAAPAVREAMGARARATVVEKFSLQRMVDAYSALIETVVSEGEARHAA"
     gene            complement(10924..12024)
                     /locus_tag="VM406_08350"
     CDS             complement(10924..12024)
                     /locus_tag="VM406_08350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVL76014.1"
                     /translation="MKVCFIGLDNLPVLAPEFNAYGVGGEQVQHTLLAKALARRGHDV
                     SMVVYDYGQPDRARWDGVTTYKAYRADSGLPMVRFVHPRWTGVWSALKRADADVYYAS
                     CAGMHLGLLAMFCRKENRRFVFRIAHDSDCDPDNLLIKYWRDRRLYEYGLRRASGILA
                     QSEQQQRALMKNYGLQSDLADMLVEAPKSQPARDVDVLWVNNLRPFKRPDLFLELARR
                     LPRYRFHMVGGKQPGHEDLYDEISVQAREIPNLEFHGPVPYHDIGPLYARARVFVNTS
                     DSEGFPNSYLQAWVRGTPVLAFFDPDGVIRRRGLGDAVESIDRMAARVDTFLRSPALW
                     SGSSAACIDYMRERYAEERILAPYLRLFAAEA"
     gene            complement(12021..13490)
                     /locus_tag="VM406_08355"
     CDS             complement(12021..13490)
                     /locus_tag="VM406_08355"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HVL76015.1"
                     /translation="MQAIQNNAALSRGHPSEGTPGILIVVAVTVAILAGALSALFGGL
                     VALIFFAVVSFMLFAISDYRAGILIAMVLLPLSASRLVPREMLGITGLNPLNVTLAMA
                     GLSVAFMWLFQRHRMAVPRIPRPFLLYGAVIAFAAAYGTTQVGMIPPFFRTLEIINFD
                     SAGGYLRDVFLKPMLMLGTAFLLAILVRNAKRPQRFLLPFFLSAMVLPLFVIGYIASS
                     GVSLGALASQQARDVLSITGMHANELGLMFNMSFALALFTVLGARGLTRWALIATCAV
                     LVVAVLLTFSRGAFLGLLTVAGIFLITQRRFKILFAGMLLLPLSILLLPDAVIERATT
                     GLENRSIAAITAGRVDDIWRPILPEISKSPVIGHGLHSMLWLEPVAAGATLKVGHPHN
                     AYLATLLDVGVGGALIVGWFFWRMRRQFAEQRARLADPLWQNFFRGATACILLLLIQG
                     ITDDRFTPTFPQTYLWLAYGMAIGLAGRLQEPDSQERTK"
     gene            complement(13537..14403)
                     /gene="epsG"
                     /locus_tag="VM406_08360"
     CDS             complement(13537..14403)
                     /gene="epsG"
                     /locus_tag="VM406_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determinant protein tyrosine kinase
                     EpsG"
                     /protein_id="HVL76016.1"
                     /translation="MNSQVATLPLKSGAEQRMGTILLEQGKLRPEDIERVLRVQKQEG
                     MIFGEAARRLGLVTEGDVQQALARQFDYHFVEEGKGKYPRELVAAYDPFSPQVEMLRA
                     VRTHLMQTWIAQGRRALAIMSAGSGEGASLFAANLAVTFSQLGQRTLLIDANMRRPRQ
                     HAIFGLTGRQGLSDILANRAGIETFSSVEGFPHLSLLPAGTVPPNPLELLGRPGFAEL
                     QASLATRFDVILIDVPAMAAASDALAVCSHTGGVWLVCRKDRARLGEVRGTTRQLARV
                     GAQVVGSALVEF"
     gene            complement(14418..>15116)
                     /locus_tag="VM406_08365"
     CDS             complement(14418..>15116)
                     /locus_tag="VM406_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determinant protein EpsF"
                     /protein_id="HVL76017.1"
                     /translation="TGTLASDSPDVVANPTVQALRVQLAAAETTFAGMASSLTEHHPR
                     YIAAKAEVDRLRADLNAVIRSSSSALANNARILERREAQLREAMEAQKARVLEANRKR
                     ADLKVLINEMETAQRSYETAAQRFMLANLEGQSNMADISVLTTALVPVAPSSPNIVLN
                     TGFAIVLGAMLAIGLALVAELLDRRVRSPQDLVDAIEAPVFEMMSWKAPPRRALAMPM
                     VLLPYSSPTSRPAN"
BASE COUNT         2766 a         4885 c         4918 g         2547 t
ORIGIN      
        1 gcccgagaaa cagcaggctg gccgcgtcgc gggtggcgta ctcggtcgcg gtcggcacgc
       61 gcaccgggtt gtagatcgag acgatcgaag gattgcggct cacgctttgc gcccatttgc
      121 gccagctctg cgagagcacg agcaggctgg ccgcgcggtc gaaggcgtag cgcatgatcc
      181 actgcttgaa cttcggcccg cgcgcgtacc aggtatcgaa gtcggcaccg tgcagatgca
      241 ggatggccgg cacgccgaac gcgtaggtcg gcaggatgaa ggtcagcttg cgccagaagc
      301 tcatgtccgt ggaaacatgg atgtggaccg ccgccacccg gcccatgagc agcatggcca
      361 tgtagcgcac ccaggctgtc gcgaagtagc ggatcttctg ggacgcgggt ccatcgacgt
      421 gcgaagcgag gtagacgatc ggaaagcgtt cgaatagccc agtctcgcgg tacacgttga
      481 cgaccgagga aatgccgccc ttcgtatgga agtcggtgcc gagcatgacg atgcgccgct
      541 tgtcgccccc tgctcgcgcc gacgtgtcga tccttgcatt catgctgcct gcgctccctc
      601 tatccgacgg aaatacggac gcatatccag atgctggcgc agcatcagcg ccttgccgcg
      661 cgcggtgcgc tcgatgcgct cgacttgcag cgcctccacc gtgcagccgg ttccgatgcg
      721 ggactccatg ctggctgcga tctgcctgag ttcatcggcg gacaggcggc gcggcatgac
      781 caggcagatc gtcagcttgc cgattgcgcg ctgctcgaac tgaatggcat gtgcgaactc
      841 cagcaacggg tgctgtcttg cggtggtgag cgcgttcagg ccgaccacac ggccgccctc
      901 gcagacgatg aattcctgaa tccgcccttc gacgcgttcg actgctggaa agcccggcaa
      961 gagcggatgc gggcggtcgg agagcatcgc catgtcgccg gtgcgatagc gcacgagcgg
     1021 catgacgtca ttgtcgaagc cggttccgac gatttctccg agctcccccg ggcgctcgat
     1081 gacgttgccg tccgcgtcga ccagttcgaa gtagccgtac tgcggccaga agaaataacg
     1141 gtcgtcatcc ggcatcgtcg cagccatcag gagacgctcc gaatgtccgt aatggcacag
     1201 cacgggacaa tcaaataccg cgcgcaggag cgtcagctga tcggcgggca cggtttccga
     1261 gaacagcatg actgcctcga tccgcccggt gacgtcgttg tccggctttt gccggaacca
     1321 ctgcgccagc gcccaggccg cgctcggata ggcctggatg aagcgtggct tccagcgacg
     1381 catcgcctcc acgtactgcg gcatgtgttc cggagacaga tgctcgttgc tgagcatgag
     1441 ctgacgcttg atcggctcgt aggcccacat gccctcgccg ttgccggctc ccggttcgag
     1501 gttcccgcgc agcgtcgcga ccacgtcatg ctcgcgcatg ccggcgcggc gatgaaaggc
     1561 ttcgatatac gcgtattcct tggcacgggt gacgcccttg tgcaggtaaa gcgtcatcgg
     1621 aagagaggtg gaaccgccgg tgaacatttt catgcgcaag gcgggatcga cgtcatgcgc
     1681 gaggaaactg ttcacgtcgc gcttgacgtc ttccttcgat atgagcggca gacagcgcag
     1741 gaccgcgacc ggatcggcca gctcggacgc cagggcgcgc cacggacgat aggcggtggc
     1801 ggagtgcagc gcgtgatgca gggtgcgggc aagcttggcc gtcgtcaaat cccgcagctg
     1861 cgcctccggg cgcggctttg cctcctcgac gaagcggcgg taattacgtc cgcgccgcca
     1921 cgacacgggg acagccgaat aggcccgtcc caccgagttt tttacccatt ggggcgactt
     1981 caggtaggaa ttgagcagag ggtgcagcaa gtcttccacg ttcatgacat ttcggctttg
     2041 cgctgcggga ggtgccaacg gtaacgcggg gaagtatcgt cgacgtggag ctggatcaac
     2101 gatccttcgc ggcggcccgg tccgtgtgcc ggccggccag cgcgcgcacc cggttcacgg
     2161 atgcggttgc cgcgcgcgtg aggaatctgt tcctgcgttc gggtgcctgc atccaggtct
     2221 tcagttgccg catccggtct tctgccacgt cgagcacgat ttgcggcgcg cgacgcacgc
     2281 agtgcagctg ggaacgatag atctcatagc gccagctggg ctgctccgca ccgcccagct
     2341 gatacttgta atcgtagcgc tgccagcccg tgtgaaagcg ccgggcgccc tgccggatcg
     2401 cctcgcacac gctgagatag gccgacagca tgcccaggct gtagcggctg tagcgttgat
     2461 cgaaggtgtt gatgaagccg aagtatccag atcccgcgcg cacgttccag gatccgccgc
     2521 agagttggcc gtcgatccag atcgtcagta gcagggaacg gctggttgcg gcgagccggt
     2581 gaaagttgtg caccatgccg gcatcgttgt agctgtgccg gcccgtgtcg ctcaggcgtg
     2641 catttgacat ggcgaccagc cgcgagatca ggtccgggtc ggcactccat ccgtcctccg
     2701 cgcgcaccgt catgctcggg tgctcgcggc gcaagcggtt gagccggttg cgcaccgtcg
     2761 agcgcgtgga ttggctcaac gaggccgtat aggcctccgt cgtatcgggc aaggcgatga
     2821 cgatatcggc acggtcggta taacagcggg cgggaaatcg ttcaagcgca tgtctgcgct
     2881 ggactgccgg catgctgacc acgcccacgt ccgggaaacg ttgaaagact gcgcccgccg
     2941 catgctccat gtcttcggcg ctgaaatcga caaactcggt cagcatcgtg gcacgtcccc
     3001 gctcaaccct gaacagcagg gtcgcggcag gcatgtcgcc gcgctccgct acaagatcat
     3061 gcacggcttg catgctttgg aacgcgcgaa cataagccgg gcttgcgaag aggtgagagt
     3121 cggatggctc gctgcagcta tcgatctccg ttaacatgcc cgatgccggg cgccagtcaa
     3181 aacgatgggg aaatttatgc gcgcttccgc tcattggtgc aggtccgttt cccggcatgc
     3241 gcgaagctcc ccctgcaggc aggggacgag caagtgcaaa aagaccggac gagtttgaaa
     3301 ggaaatggcg cagcgctggt cgacattgtc gttcggcggc gaaaacgctc tgttcgccgc
     3361 ttttcaggtg cgcgcttccc gcacgctcgc tccacacctg tgccgcgcca tccacggtgc
     3421 cccaggcacg ttggccgcag cgaacggggc atgtgatcta aggtaatccg cacgatactg
     3481 ccggtcttga gagcccgcaa aagtaaggca catcagtgcc tgcgcccgaa ggcgcaggcg
     3541 gatacgccct cagagagaca gccattcccc ggcgagacga tattccgtgc cgcgcaggtt
     3601 ctgcttgaag tgcccgaact tgtccgagga gtagtagccg ccgacatcga accagcgatg
     3661 gccgagccgc ttggcttcgc agatcgcgtg ccagtagagg aagttgccgc agttgagctt
     3721 gcgaccgtcg tcaccgaacc agcccacgta gtactcggca cgatccccga agcggtacag
     3781 gaccagcccc cctaccgcac gactgcccgc catcgcctgc aggatgcgga aatcgccggg
     3841 cttggccatg tagagcgcct tgagcagcgc acggctcgca ccgttaaatc gcttggtacg
     3901 ctggttttgt tcgtgccggt ccagcatcca ttccaggccc tgtacgtcat tgcagtgacg
     3961 gatttcgagg ccgctcttct gtgcagccct gaggcgattg cgccataccg gcgcgaagcg
     4021 cttttgaatt tcttcctccg ggggcgagag gtcgacgagc gcggaacacc agccctgctc
     4081 cttgcgcagg cgatagccta gccggtgcag cagcgcccga ttctccgatg tatcgtccag
     4141 tgccggcgcg atgagcaggg ctccgtgtct cccgatgcgc cagtatctgc ggatggcctg
     4201 catcacgcct tcgcgtagcg cgagagacgg cgtctcgtca aagaaaagcg gaccgcgatt
     4261 gatgcgtgcg ccgagcgcaa tgcccgctgc gcgcaggacc agcgcctgga ccatggccac
     4321 cggcgcgccg ttgtactcga tgaggtagcg ctgcgcatgc agcgatcctg tcgcggcctt
     4381 ggcctcgcca taggcatggg cctgcgtcat atgcgtgcgc ggcaaccggg aaaaatgaca
     4441 ctcccattcc ttcagggagc ggatcgaccg cacttggtat atgcagtgtg acgtggactg
     4501 ggcgtcgagg gaaagggaaa ggcgcacgtt tcacctattt gttggcggcg gtttttggat
     4561 gctgcgcgcg gcgcagagtt tgtgcaggcc tgcgatcagc aaactgccgc tgtggcgccg
     4621 agtagcgtgg tgacgcagag gcggcgctgt gcacagcggc tttccgtcgc tgctgcgacg
     4681 cctggcacgg atggaatagg ggatgcagca ggacttcaat gttcacgttg ttgttcgact
     4741 atgccaatca attcgacggt tcaacgttac tagagagcgc gtcgcacatc cccgatattg
     4801 atcaacaccc cgcggccgtg cacagcccgc gcgcgccgcc atacaagaaa aaatcacgca
     4861 acaggtcgtt gccggaagca ggcgcatgca gtgcgctcaa ccgtcccctc atcccgagtt
     4921 cgtcattttt cttcaggcgg acaccgcaca tgtcagagtc ccagccattc cccggccagc
     4981 ctgtactcgt ggccgcgcat gttcatcttg aagtggccga acttgtcatt ggtgtcgtaa
     5041 ccgcccacgt cgaaccagcg atatccagcc ttcttcgatt cgcatatcgc ctgccagtag
     5101 aggtaattgc cgctctggag cttgcgtcct tccggaccga accagccgac gtagtgctcg
     5161 acggtgtggc cgaagcgcgc caggatcatg ccggccaccg ggcgatcctg atgcagcgcc
     5221 tgcaggacct ggacctcgtt cgggtcggcg gcgtgcagcg ccagcagcaa atctggcttc
     5281 gggccggcga agcccttggc gcgcatgttt tcctggtgcc gctcgaccat ccactggtac
     5341 gagccgtcgc tttgcgagac gcgcagttcc aggccgctct tcatggcccc cttgaggtga
     5401 ttgcgccacg acgaactgag ccgcttgcgt atttcttctt cggacaggcc gaggtcgatc
     5461 agcgacgagc accagccccg ttcacgccgg cagcgaaacc ccagcgaacg cagcaacgtg
     5521 cggttttcct cggagtccgg cagcgccggc gcgatcagca acggaccgcc ccgtccggtg
     5581 cgccagttca tgcgcacgag cttcatgacg ccctcgcgca cgccatacga aggtgcgctg
     5641 tcgaggaata ccgggccgcg attgatgcgg gcgccgaccc gcacgcctgc caggcggcgt
     5701 tccagcacct ggcagatcgc cactggcgtg cgcccctgtt cgaacacgta gcgctcgaca
     5761 tgcaggcgct cggcggcgcg cttggcctcc ccgtacggat aggactgcat caagtgcgcc
     5821 tggggcaacg cgtggaagta ttccccccat tccacggcgc tggtcaccgg ccgcatccgg
     5881 tacgactgca tcgcagccgc cgacggcgca aacatcgttg tggtttgcat gcgctggcac
     5941 cctttcatca tcggttcgca aacttggact caggatccgg aaagcagcaa cacaccgtcc
     6001 tgcggcgcca gctcgatgct cgaaaccgcc cgtccggaat tgatttgtgg cgcctgctcg
     6061 ccgcgcaggt gcctgtaacc cggaccgata tcgacccttc tggtttcgcg ggtcggattg
     6121 acgaggacca agccgtgctc gaagcgtcgc cggtacacgc cgttcttcca ggggctgcgc
     6181 tgcggcgggt cgatcgcgcg gccgagcctg gcgtcgtatt cgtcgaacca tggcacgctg
     6241 ctgtagccgc gcgcctggtc ggtgtaggag aaatagccgt cgttcatcag gcaggaggcg
     6301 agcgcaaagc gcatcagccg gtagtcgtcg gcagcgcccc agacattgaa cgagacgatg
     6361 cgcggcgctg ccaggttgtt gaacacgctg tggtagcgcg tcatcatcgc gttccagccg
     6421 agtcgctcgt aaagcgacca cgactcgccc atcagcgttt caagaaacgc gccctgcagc
     6481 cggttgcgga attcgggata cgataggtcg ttgtccgtgt tgccgatgaa cagccgatgc
     6541 ggcgacagac gccgcgcggc ggcccagtgc tcggcctgtg cgcgccggaa cgcgcgctgc
     6601 acctcgggat cggtgcccga ctggttgcgc ccgctgcccc gccagtcggc aagacggatg
     6661 cgctgtgcgt gcatcacatt gtcgaaaaac cagatgtcga actcgggaac cggttcgaag
     6721 tagacgcgat ggtcgcgcag cgccagccac tgcgggtagc gcaggccgtt gcggtccttg
     6781 cgcgtccatg cggtgatgtt gatatcccag gcgccgtaga tctcggtcca ttgcaccggc
     6841 ttgcccgccg ccgtggtcag ccaccagccg ttttcgcgca gcgccgcctg cttgtcctgt
     6901 tcggcggtgt tgtccgcgtc gttgctcgct tcattgagta tcgtgtactg cccgacaagg
     6961 atcgagggat tgagacgcct gagctcgcgc accacctcgc gcatcgcgcc cgggcgctcg
     7021 ccccagccgc ggtagaagcc gaggatgacg atgtcgagcc gcgccatctg ccgctggtaa
     7081 tcggggtcgt cgtagtgctt ctcgccgatc tgcatgccga gcaggcgcgg aaacccgggt
     7141 tcgggccgcc tggtaagacg ttcgtcggcg ccgccgcgtg cgaaggaaga ggccgccgtg
     7201 acgagcacga gcaggaacaa cagcaagcgc atcttgtggc ctcatgcaag cgcaggcatg
     7261 tcgtcctcga gacgttcatg gcgcaacagg cacttggtga cgaagcgcgc cagctccagc
     7321 acgccgggct gccagtgctt gagcagcggt gacgaacgcc acacccgcag cgcttcgcgt
     7381 cccagcggcg atgcgcactt gcagacctgg taccactcgt agccgagcag gcgcttgaaa
     7441 tccggatccg ggtagcgctg cagctcccag ttggccaggc agagcttgag cagccatgcc
     7501 tccacgtcct ggagcagctg cacggtcgga aacagcgtgt ggccgccgac atgccggttc
     7561 accgccagtt cgcgctcgct cgcctccacg ccgaagcggc gcaactggct gcgcatgatc
     7621 ccccgcatgc agttctccgc gagcaccttc tgttcccagc tcggcttcga gggcagcagt
     7681 cggacagcgg tgagcgtcct gtcgagattg cataccttgc cgccggcggc gacgcgggca
     7741 ttgaaatcga agtccacgcc ggcgggcaac gcgcggaatc cgccttcggg catcgcgctt
     7801 ttccggaaag tgacccccga caaggcgtcg cgaaacagca tctggatgcg cgtctcggcc
     7861 gcactggtgg gccgcatcca gatatcgccg agcttgccgt gcgcgttgat tacggccaga
     7921 tgggagccga ccaggcgcag ttcgggatcg gcgtcgagcg cggcgacttg ctcggccagg
     7981 cgttgcggca aggcgcagca gtgcgcgtcg agcagggcga tgtactcgcc gcgcgcctcg
     8041 gcaatgccaa gattgcgggc catcgcgagg ccgcagtggc gtacgttctg caccagccgc
     8101 acacgctcgt ccggccagct gcgcgcgatg tcggctgtcg cgtcggtgga tccgtcgtcg
     8161 accaccacga cttcgaaccg gtcgaagtgc tgccgccgca ggctctccag cgcgctgcgc
     8221 agggtggcct cggcattgaa cgccgacatc acaaccgtca cacgggggtc atgcggtttc
     8281 atgtgcacct ccttcgacga cagccttgtc cttgcggaag ccggccagct cggtggcagc
     8341 tcgccaaccg ccagccagca ggaacacggg cacgaatgcc acgcagtaca caccggcctt
     8401 ggccagaagc atgttccatg gagccagggt ccggtcacca gtcacccact cgaccatcaa
     8461 cagggcgaaa gcggtcgcca gtatggcagg cagcagcgag tgccaattga tcaggcgcag
     8521 agccgatgaa tgcagtgtgc gcgcaacgac cagcaggccc cacagttcgc cgacggccag
     8581 cgcaatcatc gatcccgccg cagcgccggc catgccgaac tgtctcgtca gcgttacaca
     8641 cagcacgatg gacagcgcca ggcagaccgc gctgttgatg gcagcaaaac ggccatggcc
     8701 gagcgccgac agcgtatggc cgacggcgat cgccgtgatc atcaggccga tcaggtacac
     8761 gcgcatcacc ggcgcagcct cgatatagcg ccgcgtgtac accagttcga ccagttcggg
     8821 cgcgcaggca aacagcgcgc cggcaacggg aatcaacagc atgccggagg cgccgttgga
     8881 cagcgcgatc agccgtgcgg cctgtgcctt gtcaccggca ccaaacacct tgttcaggcg
     8941 tggcagcagt gcgttgttga ctggctgccg gatcagcgtg ccgatcggaa gaatcacggc
     9001 cgcaaccgag aacgtcgcat acatcgcggt cgggagcatg ctgacgacca cccactggtc
     9061 cgcttgcacg cgcaggagga acagcgtatg gccgatcgcg aacggaaacg cgtacatcac
     9121 ctgggtgcgc agcaacgcaa gatcccattc cgagtcggtg cgcgcgatga aacgccgcat
     9181 gtagacggcc aggagaagaa gcttggcgct ggcgaccgcc agcatcacca cggcgatcca
     9241 gtagatgtcg cccgcgaaga gtgcagccgc ggccagcagg acagtgcgca gcaccgccag
     9301 cccgatgatc gcgccggcct gccaggcggc gcgtccgtcc gcggtcggca gcacgtcgag
     9361 catcgatgcc atgacccaca gtgcgaggaa ggccgcggac aaacccgcgg aatcgcgaaa
     9421 caggtcgagc gccgcctgct ggagccaggg gttgagatcg ctggccacca gtgccgacag
     9481 cgtgcccgca gccgtcagga acagcagcac gttgccgatg taggaggcct tgcgccggcg
     9541 ccgtgcccgg ctgaggaaat agaacagcga ctgcggcatg aacatcggtg caatcgccag
     9601 cgcggtgccg gcaagcagcc acaacagccg gtattgcgca aacgtgattt cgtcgagata
     9661 gcgcgcgagc aggatgggaa tggccatctg cagcgcaaac tcgatcattc ctgcgaaccc
     9721 gagcaggacg gcgctggtgc gcaggctctc gctacgcggc atgacgggcc tcgccctcgc
     9781 tgacgacagt ttcgatcagt gcggaatagg cgtcgaccat gcgctgaagc gaaaacttct
     9841 cgactacggt ggcgcgtgcc cgcgcaccca tcgcttcgcg caccgcgggc gcggccagcg
     9901 tgtgcaatgc ggcgcacagc gcggcgatgt ctgcgcgccg gtacaggaag ccgttgtcgc
     9961 catggcggat ctgctcggcc gctccgccga tgtcggtcat caccatgcac ttcccgagcg
    10021 ccatcgcctc gagcgcggcg atcgagaacg tctcgacgtg gtgcgaggtg atcgccatca
    10081 cgtcgcaggc ggcgatcagc ggtctcacgt cacttgcgaa gccggtaatg catgcatgcc
    10141 cgccaagacc cagcctttcg atcaccgctt cgatctccgc tcgcatcgga ccgtcgccga
    10201 tcagcagcgc acgcgcccgc accccttcgt cccgtagccg gacgagcgcg cgcaacaggt
    10261 cgccatgcgc cttttccgga cgcatcgtcg cgcacaagcc gatcacatag tcgccgcttc
    10321 ccaagccgta cgcggcgcgc accgctgcct tctccctgtc gctgtagccg tcggcgaagt
    10381 gcgcgatatc gatgccgttg tgaatcactg cgtcgccgcg ggcgcgcagc gcgcggcggc
    10441 gccagtagcg tcgctggttc tcgcagacat agaccaggcg gtcacaggcg aggatccatg
    10501 gtcgatagaa cagcatctgc agttcatctt cgagcgatcc cagttcggtc gagtggaaca
    10561 cctcgacaat gcgcggacgc ggaccgccgg atgcggcgct ggccatgcgc gccagccagg
    10621 cgtacagcag cgaataggta ttcgtgcaca gcacaatgtc gatgtgctcg ccgcgcaggt
    10681 ggtcagccag gcgccgggcg gcggcccggt ctatcttgcg cgacaccccg gcgcagaaca
    10741 cgccgccttc ggcgcgctcg ctcatgatct gaggcagcag cgcatcttcg ctcttcaggt
    10801 aggcgaacga gagcctgaag cggctggtgt cgagcgtgtt gagcagcgtg accgcatgct
    10861 tctcggcacc acccacgcac aaggaattga cgatcagcag gacatggatg cggcgcccgt
    10921 tcatcacgcc tccgcggcga aaaggcgcag gtaaggcgcg aggatgcgtt cttcggcata
    10981 gcgttcccgc atgtagtcga tgcaggcggc gctggagccg gaccacagcg caggagaacg
    11041 caggaaggta tcgacgcgcg cggccatgcg gtcgatcgac tcgacggcat cgcccaggcc
    11101 gcgccgcctg atcacgccgt caggatcgaa gaacgccagc accggcgtgc cacgcaccca
    11161 tgcctggaga tacgagttcg gaaatccctc gctgtcggag gtattcacga acacgcgcgc
    11221 gcgtgcatag agcgggccga tgtcgtggta gggaaccggg ccgtggaact ccaggttcgg
    11281 tatttcccgc gcctgtacgg atatctcgtc gtacagatct tcatggccag gctgcttgcc
    11341 gccgaccatg tggaagcgat agcggggcag gcggcgggcc agttcgagga agaggtcggg
    11401 ccgcttgaac gggcgcaggt tattcaccca cagcacatcg acatcgcgcg cgggctgcga
    11461 cttcggagct tccaccagca tgtcggccag atcgctctgc aagccgtagt tcttcatcaa
    11521 cgcgcgctgc tgctgctcgc tttgcgccag gataccgctg gcccggcgca ggccgtactc
    11581 gtagaggcgc cggtcgcgcc agtacttgat cagcagattg tccgggtcgc agtcgctgtc
    11641 atgcgcgatg cgaaatacga agcgccggtt ttccttgcgg cagaacatcg caagcagtcc
    11701 cagatgcatg ccggcacagc tggcgtagta gacatccgcg tctgcgcgct tgagcgccga
    11761 ccagacgccg gtccagcgcg gatggacgaa gcgcaccatc ggcaggcccg aatcggcccg
    11821 gtatgccttg taggtggtca ccccgtccca tcgggcacgg tcgggctggc catagtcgta
    11881 caccaccatg ctcacgtcat gtccgcgccg tgccagcgcc ttggccagga gcgtatgctg
    11941 cacctgctcg cctccgacgc cgtaggcatt gaactcgggc gccagcaccg gaagattgtc
    12001 caggccgatg aagcacactt tcacttcgtt cgctcctggc tgtccggttc ctgcaggcgc
    12061 cccgccaaac ctatcgccat gccataggcg agccagagat aggtctgggg aaaggtcggc
    12121 gtgaagcgat cgtcggtgat cccctgtatc agcagcagca ggatgcaggc ggttgcgccg
    12181 cgaaagaagt tctgccacag cggatcggcc aggcgggcgc gctgttcggc gaactgccgg
    12241 cgcatccgcc agaagaacca gccgacgatc agcgcaccgc ccacaccgac gtcgagcagc
    12301 gtggcgagat aggcgttatg ggggtgcccg accttcagcg tggccccggc ggcaaccggt
    12361 tcgagccaga gcatcgagtg caatccgtgt ccgatcaccg ggctcttgga tatctccggc
    12421 aggatagggc gccagatgtc atccacccgt ccggcggtga tcgcggcaat gctcctgttc
    12481 tccaggccgg tggtggcgcg ttcgatcacg gcgtcgggca gcagcagaat ggacagcggc
    12541 agcagcagca tgccggcgaa caggatcttg aagcggcgct gggtgatcag gaagatgccg
    12601 gccaccgtga gcaagccgag gaaggctccg cgcgagaacg tcagcagcac ggccaccacg
    12661 agcacggcgc aggttgcgat caatgcccat cgcgtcaggc cgcgcgcgcc aagcacggta
    12721 aacagcgcca gcgcgaagct catgttgaac atcaggccga gttcgttggc gtgcatgccg
    12781 gtgatggaca ggacatcgcg cgcctgttgc gaggcaagcg cgccaagcga gacgccggaa
    12841 cttgcgatat agccgatcac gaacagcggc agcaccatcg ccgacaggaa gaacggcagg
    12901 aggaagcgtt gcgggcgctt cgcattgcgc accaggattg cgagcaggaa ggcggtgccc
    12961 agcatcagca tcggcttcaa gaacacgtcg cgcagatatc cccccgccga atcgaagttg
    13021 atgatctcca gcgtgcggaa gaatggcggg atcattccca cctgcgtcgt cccgtaggcg
    13081 gctgcgaatg cgatcaccgc gccataaagg aggaacggcc ggggaatgcg tggtaccgcc
    13141 atacggtgcc gctggaacag ccacatgaaa gcgaccgaca agccggccat cgcaagcgtg
    13201 acgttcaacg ggttcaggcc ggtgatgccg agcatttccc gcggcaccag gcgcgacgcc
    13261 gacagcggca gcagcaccat cgcgatcagg atgccggcgc gatagtcgga gatcgcaaac
    13321 agcatgaagc tcacgacggc aaagaaaatc agcgcgacca atccgccgaa cagcgcggac
    13381 agcgcgccgg caaggatggc caccgtgacc gccacgacga tcaggatgcc gggagtgccc
    13441 tcactggggt gtcctcgaga gagggcggca ttgttctgga tggcttgcaa ggcaatctcc
    13501 atggagcacg gccgtatgcg gccggaggca cgccactcag aattcgacca gcgccgagcc
    13561 gaccacctgg gcgccgacgc gcgcgagctg cctcgtggtg ccgcggacct cgcccaggcg
    13621 cgcacgatcc ttgcggcaca ccagccacac gccgcccgta tggctgcaga cggcgagcgc
    13681 atccgatgcc gcagccatgg ccggcacgtc gatcaggatg acgtcgaaac gggtggccag
    13741 gctggcctgc agttcggcaa agccgggccg tcccaacagt tccagcggat tgggcggaac
    13801 agttccggcc ggcagcagcg acagatgcgg aaatccttcc acgcttgaga acgtctcgat
    13861 gccggcgcgg ttggcaagga tgtccgacag gccctggcgc cccgtcaggc cgaagatcgc
    13921 atgctggcgc gggcgccgca tgttcgcgtc gatcagcagc gtgcgctggc caagctggga
    13981 gaaggtcacc gccagattgg cggcaaacag gcttgccccc tcgccgcttc ctgcgctcat
    14041 gatcgcaagc gcgcgccgcc cctgcgcgat ccaggtctgc atcagatggg tgcgcaccgc
    14101 gcgcagcatc tccacctggg gactgaacgg atcgtaggcg gccacgagct ctcgtggata
    14161 ctttcccttc ccctcttcca cgaagtggta atcgaactgc cgcgcgaggg cctgctgcac
    14221 gtcgccttcg gtgaccagcc ccagccggcg cgcggcctcc ccgaagatca tgccctcctg
    14281 cttctgcacg cgcagcacgc gctcgatgtc ctccgggcgc agcttgccct gctcgagcag
    14341 gatggtgccc atgcgctgtt ccgcacccga tttcaaaggc agggtcgcta cctggctgtt
    14401 catggtcttc tcccttttca gttcgctggt cggctcgtcg gactcgagta aggcaggagc
    14461 accatgggca tggccagggc gcggcgcggt ggcgccttcc agctcatcat ctcgaacacg
    14521 ggcgcctcga tggcgtcgac gagatcctgc ggcgagcgca cgcggcggtc gagcagttcg
    14581 gccaccagcg ccagtccgat ggcaagcatg gcgccgagca cgatggcaaa tcccgtattg
    14641 agcacgatgt tgggcgagct gggcgcgaca ggcacgagcg cggtggtcag caccgagatg
    14701 tcggccatgt tcgactgccc ttccagattg gcgagcatga agcgttgcgc cgcggtttcg
    14761 taggagcgct gggccgtctc catttcgttg atcagcacct tcaggtccgc gcgcttgcgg
    14821 ttggcttcga gcacgcgggc cttctgcgct tccatcgctt cacgcagctg cgcttcgcga
    14881 cgctcgagaa tgcgggcatt gttggccagc gcgctggagc tggatcggat gacggcgttc
    14941 agatcggcac gcaggcggtc cacctccgcc ttggcggcga tgtagcgcgg atgatgttcg
    15001 gtaagcgagc tggccatgcc ggcaaaggtc gtctcggcgg cggccagctg aacgcgcaag
    15061 gcctgcaccg tgggattggc gaccacgtcc ggggagtcgg aggcgagggt gccggt
//
LOCUS       DATLGF010000274         8130 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_195286, whole genome shotgun sequence.
ACCESSION   DATLGF010000274 DATLGF010000000
VERSION     DATLGF010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 8130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8130
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195286"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VM406_08370"
     CDS             complement(<1..282)
                     /locus_tag="VM406_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HVL76018.1"
                     /translation="MAIKAENLRMFRKLAVIAAGMFAFGYAIIPIYRHICEVTGINIL
                     ALGEKQVPGGSTAAANSQVDRSRTITVEFDANSRGPWEFKPQVASIQVHP"
     gene            complement(287..403)
                     /locus_tag="VM406_08375"
     CDS             complement(287..403)
                     /locus_tag="VM406_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase small assembly protein"
                     /protein_id="HVL76019.1"
                     /translation="MAQNDERRKANRRLGLILASVALAFAIGFAAKIVLLGG"
     gene            complement(420..1289)
                     /locus_tag="VM406_08380"
                     /pseudo
     CDS             complement(420..1289)
                     /locus_tag="VM406_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902719.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
     gene            1276..2259
                     /locus_tag="VM406_08385"
                     /pseudo
     CDS             1276..2259
                     /locus_tag="VM406_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761054.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
     gene            2297..2407
                     /locus_tag="VM406_08390"
     CDS             2297..2407
                     /locus_tag="VM406_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase small assembly protein"
                     /protein_id="HVL76020.1"
                     /translation="MQDPEKRKKNIRTALITASIALAFFIGLFIKRTWFV"
     gene            2409..3005
                     /locus_tag="VM406_08395"
     CDS             2409..3005
                     /locus_tag="VM406_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HVL76021.1"
                     /translation="MENPQLEEKTVTRRLNAQMMGKLVVVAFLMFGFGYALVPIYEKI
                     CEITGVNLLTPKMNISLDEIKNTQVDTSRTITIEFDATANGPWRFRPTVSSMEVHPGE
                     MAQVVYEVVNTQPRAIDAQAIPSYAPQQAAAHFKKVECFCFQQQTMEAHQAKQMPVVF
                     FIDPALPKDVKTITMSYTFFEIAGRNQAAASTVKPTDG"
     gene            2998..3210
                     /locus_tag="VM406_08400"
     CDS             2998..3210
                     /locus_tag="VM406_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003269927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HVL76022.1"
                     /translation="MDEKDKPAPRKAGFGATMKAVLWSFFGVRKRSDYERDAEQLNPV
                     HVIIAGLLAALGFVVTLILIVRAVVG"
     gene            3254..4111
                     /locus_tag="VM406_08405"
     CDS             3254..4111
                     /locus_tag="VM406_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HVL76023.1"
                     /translation="MDSRQANAPYYFVPGPSPWPVAAGASLLVTMAGASGWVNEQAWG
                     MYLSLAGVLATLFVMYRWFGDAIAESEGGLYSTRIDVSYRWSMSWFIFSEVMFFAGFF
                     GALFYARAVTLPWLGDLDHRILWPDFAAEWGNSPGGIVEPFERMGPFWIPTINTALLL
                     TSGVTLTIAHHALRAGHRSKTIVWLAATILLGIVFMGFQAYEYVHAYRDLNLRLDSGI
                     FGSTFYMLTGFHGFHVTLGAIMLAVVLYRVVKGHFTPENHFAFEGAAWYWHFVDVVWL
                     GLYVVVYWM"
     gene            complement(4204..4407)
                     /locus_tag="VM406_08410"
     CDS             complement(4204..4407)
                     /locus_tag="VM406_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HVL76024.1"
                     /translation="MKAFVAVAFIFILGSLTSALVFLMKDKGRSNRTVTALAFRVGFS
                     IALFLLILLAYYFGWIRPTGFVR"
     gene            4407..5174
                     /locus_tag="VM406_08415"
     CDS             4407..5174
                     /locus_tag="VM406_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HVL76025.1"
                     /translation="MEAFPARRARGVRKSDIVMALNFRVRWVPLIAALAAASVGIALG
                     DWQTRRAQEKEAIEARLSVRETEAPVQLSAYAGDPAELEFRRVLMRGEFVSGWNVFLD
                     NRPYAGAAGFYVAAPFRLEHDNSHVLVLRGWTPRNIAERTRIQEIPTPDGSIEIEGMV
                     RMHIGRIMQLGDPEPLRPGAIVQNLVIEELGRAGGWTMRPFVVEQLSDTGDGLVRDWL
                     RPSTGADKHRGYAFQWYGLAAAALVFFFVTGFRRGPK"
     gene            5161..5757
                     /locus_tag="VM406_08420"
     CDS             5161..5757
                     /locus_tag="VM406_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit I"
                     /protein_id="HVL76026.1"
                     /translation="MDQSSKQKRNSGRWKLFAVIAICASPLIASYFTYYVIKPEGRTN
                     YGTLLDPRAYPMPVLKAKTLDGIPAELETLRGKWIMLQVAPGACDDACRKRLHDMRQL
                     RLAQGKNMDRVERVWLINDASPLETLVMREYDGTHMLRADAQAIAAWLPAETTTTESD
                     HLYVIDPLGNLMMRFPKDADPNRIKKDLSRLLRASSIG"
     gene            5762..6871
                     /locus_tag="VM406_08425"
     CDS             5762..6871
                     /locus_tag="VM406_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HVL76027.1"
                     /translation="MLVQLALKGLLAALIPLLIVWTARDSNKYRKLVWVTAFLTFDLI
                     MFGAFTRLTDSGLGCPDWPGCYGYANPFQAIEHIRAAEALMPDGPVTVMKAWIEMIHR
                     YFAMGVGVLIIAIMTIAWRNWFRTQRQDARYAPGFPTFLLFFVCVQGAFGAWTVTMKL
                     QPVIVTIHLLLGMGLLAALVWLGARQNDYPPVVAARSLRPLAAAGLAVLFIQIGLGGW
                     VSTNYAALACLDYPLCNGQFIPDMDFEHGFSLWRELGKTRGGEYLPFPALVAIHWVHR
                     MFAIVVLAVLLALALHARRVAELRRLANLLMLAIGIQFLTGMATIFLKWPLALAVIHN
                     GGAAVLVVLLTVLNYRARLAVPAEAQPGTTRLSPA"
     gene            6882..7766
                     /gene="cyoE"
                     /locus_tag="VM406_08430"
     CDS             6882..7766
                     /gene="cyoE"
                     /locus_tag="VM406_08430"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HVL76028.1"
                     /translation="MTTLTAHPNRVAQYWALTKPRVTQLAVFCAVIGMFLASPGLPDW
                     RIVVAATIGIWLLAGAAFAVNCLVEREIDSRMARTARRPAARGEITVMQILVFSGVIG
                     GAGMWVLFNFVNPLTMWLTFATFVGYAVIYTMLLKPMTPQNIVIGGLSGAMPPALGWA
                     AVANDVPMEAWILVLIIFVWTPPHFWSLALYRRDDYAKSGLPMLPITHGLKFTQFQIW
                     LYTIALVATTLLPFAVRMSGLIYLVSALVLGAIFLWYAWQVWRHYSDLIARKAFAYSI
                     VYLSLLFAALLVDHYLKF"
     gene            7768..>8130
                     /locus_tag="VM406_08435"
     CDS             7768..>8130
                     /locus_tag="VM406_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HVL76029.1"
                     /translation="MSYSVRRIAALALIAGSVLVAGCDRLGASGGTQFQNTDLTGLEY
                     ARGFELPDHNGQLRTLADFRGKAVVVFFGFTHCPDVCPSTMAEMAQVMEQLGPDAERV
                     QVLFVSVDPQRDRPEILRH"
BASE COUNT         1376 a         2544 c         2620 g         1590 t
ORIGIN      
        1 cggatgcacc tggatcgacg ccacctgggg cttgaactcc caggggccgc gcgagttggc
       61 gtcgaactcc accgtgatgg tgcggctgcg gtcgacctgc gagttggccg cggcggtgga
      121 gccgcccggc acctgcttct cgcccagcgc caggatgttg atgccggtga cctcgcagat
      181 gtgccggtag atcgggatga tggcgtagcc gaacgcgaac atgccggcgg cgatcaccgc
      241 cagcttgcgg aacatgcgaa ggttctcggc cttgatcgcc acggcgtcag ccccccagca
      301 gcacgatctt ggccgcgaag ccgatggcga aggcgagcgc caccgacgcc aggatcagcc
      361 ccaggcggcg gttggccttc ctccgctcgt cgttctgcgc catcgccagc acctcgtcct
      421 cagtgggccg cgcccgcgtt ggggttgacg gacgagggca gcgcgtccag cacgcgggtg
      481 gcgctggcgt ccagccgggg cggcacctcg taggtgtgga agggcgccgg cgacggcact
      541 tcccactcca ggccttcggc gccgcggccg tccggatcgt tccaggggcg ctggccggcc
      601 ggctcgccgc ggccgcgcat cacgggcagg accacgaaca ggaagaagta cacctgggcc
      661 aggccgaagg cgaaggcgcc cagcgaagcc cacatgttga agtcggcgaa ctgcatcggg
      721 tagtcggcgt agcggcgggg catgccggcc agcccgagga agtgcatcgg gaagaaggtg
      781 acgttgaagg agatcagcga ccaccagaag tggatcttgc cgcgcgcctc gttgtacatc
      841 acgccggtcc acttgggcgc ccagtagtag aagccggaga acagcgcgaa cagcgagccc
      901 gccaccagca cgtagtggaa gtgcgccacc acgtagtagg tgtcctgcag cagcaggtcg
      961 atgggggcca tggccaggat caggccggtg aagccgccga tcgtgaacac gaagatgaag
     1021 ccgaccgcga acagcatcgg cgtttcgaac gtcatcgagc cctgccacat cgtggcgatc
     1081 cagttgaaga tcttcacggc cgtgggcacc gcgatcagca tggtcgcgta catgaagaac
     1141 agctgccccg tcaccggcat gccggtggtg aacatgtggt gcgcccagac gatgaacgac
     1201 aggatggcga tcgagctggt ggcgtacacc atcgaggcgt agccgaacag gcgcttgcgc
     1261 gagaacgccg gcacgacctg gctgatcacc gcctacctgc tgatcgccgt gatgccggtg
     1321 ctggctggtg cgatcacgat gacgctgacc gaccgccact tcggcaccac cttcttcaac
     1381 gccgccggcg gcggtgatcc ggtgatgtac cagcacatct tctggttctt cggtcacccc
     1441 gaggtctaca tcatgatcct gccggcgttc gggatcgtct cgcacatcat cccggcgttt
     1501 gcccgcaagc cgctgttcgg ctacgcgtcg atggtgtatg cgaccgcctc gatcgcgatc
     1561 ctgtcgttca tcgtctgggc gcaccacatg ttcaccaccg gcatgccggt gaccggccag
     1621 ctgttcttca tgtacgcgac gatgctgatc gcaatcccga ccggcgtgaa ggtgttcaac
     1681 tgcatcgcca cgatgtggaa gggttcgatg accttcgaga ccccgatgct gttcgctgtc
     1741 ggcttcatct tcgtgttcac gatgggcggt ttcaccggtc tgattctggc ggtgaccccg
     1801 atcgacatcc agctgcatga cacctactac gtggtggcgc acttccacta cgtgctggtg
     1861 gccggctcgc tgtttgcgct gttcgccggc ttctactact ggggcccgaa gtggaccggc
     1921 ttcatgtacg acgaaacccg cggcaagatc cacttctggg gttcgctgat caccttcaac
     1981 atcaccttct tcccgatgca cttcctcggt ctggccggca tgccgcgtcg ctatgccgac
     2041 tacccgatgc agttcgccga cttcaacctg atcgcgtcgg tcggcgcatt cggcttcggc
     2101 ctgatgcagg tgtacttcct gttcgccgtc gtgctgccgt cgatccgcgg cggcgagaag
     2161 gcggcagaca agccgtggga aggtgcggaa gggctggagt ggaccgtgcc gagtccggcg
     2221 ccgttccaca ccttcgaggt gcctccgacc gtcaagtaat gtgagcacgg cgggcgcaag
     2281 cccgccgtga tgcgtgatgc aagaccctga gaagcggaag aagaacatcc gcacggcgct
     2341 gatcaccgcg tctattgcac tggccttctt catcggcctg ttcatcaagc gtacctggtt
     2401 cgtctgacat ggaaaatccg caactcgagg aaaagaccgt gacccgacgg ctcaatgccc
     2461 agatgatggg caagctggtg gtggtggcgt tcctgatgtt cggtttcggc tatgcgctgg
     2521 tcccgatcta cgagaagatc tgcgagatca ccggcgtcaa cctgctgacg ccgaagatga
     2581 acatctctct cgacgagatc aagaacaccc aggtcgacac ctcgcgcacc attacgatcg
     2641 agttcgacgc gacggccaac ggtccgtggc gtttccgccc tactgtttcc agcatggaag
     2701 tacatccggg cgagatggcg caggtcgtct atgaggtcgt caacacccag ccgcgcgcga
     2761 tcgacgctca ggccattccg agctacgcac cgcagcaggc ggcagcgcac ttcaagaagg
     2821 tggagtgctt ctgtttccag cagcagacga tggaggcgca ccaggccaag cagatgccgg
     2881 tggtgttctt catcgatccc gcattgccga aggatgtaaa gacgatcacc atgtcctaca
     2941 ctttcttcga gatcgccggc cgcaaccagg cggctgcctc gacagtgaag ccgacggatg
     3001 gatgagaaag acaagccggc cccgcgtaag gctgggttcg gtgccacgat gaaggctgtg
     3061 ctgtggtcct tcttcggtgt gcgcaagaga agtgattatg agcgggatgc cgagcaactc
     3121 aatccggtgc atgtcatcat cgccggcctg ctggcagccc tgggcttcgt cgttacgctg
     3181 atcctgatcg tgcgtgcggt ggtgggctag acaagaattt atacatactg aattgcatac
     3241 tgggagattt gagatggatt ctcgacaagc gaacgcgccg tactatttcg tgccaggccc
     3301 gtcgccctgg ccggtagcgg caggtgcgtc cctgctggta acgatggcgg gcgcgtccgg
     3361 ttgggtcaac gagcaggcgt gggggatgta tctgtccctg gccggcgtgc tggcgacgct
     3421 gttcgtgatg taccgctggt tcggcgatgc aatcgccgag tccgagggcg ggctgtacag
     3481 cacccgcatc gatgtttcct accgctggag catgagctgg ttcatcttct ccgaggtgat
     3541 gttcttcgcc ggcttcttcg gcgcgctgtt ctatgcccgc gcggtcacgc ttccgtggct
     3601 gggcgatctt gaccaccgca tcctgtggcc cgatttcgcg gccgagtggg gcaattcgcc
     3661 gggcggcatc gtcgagcctt tcgagcgcat gggcccgttc tggatcccga ccatcaacac
     3721 tgcgctgctg ctgacttccg gcgtgacgct gaccatcgca caccacgcgc tgcgcgccgg
     3781 tcatcgcagc aagaccattg tctggctggc ggcaacgatc ctgctgggca tcgtcttcat
     3841 gggcttccag gcttatgaat acgtgcatgc ctaccgcgac ctgaacctgc gtctcgactc
     3901 cggcatcttc ggttccacct tctacatgct gaccggtttc cacggtttcc atgtgacgct
     3961 cggggcaatc atgctggctg tcgtgctgta ccgcgtggtg aaggggcact tcacaccgga
     4021 aaaccatttt gcgttcgaag gtgccgcctg gtactggcac tttgtagacg tggtctggct
     4081 cgggctgtac gtggtcgtgt actggatgta attcatcccg gccaagaaaa agccgcacct
     4141 cggtgcggct tttttcactg tgggcgggac gattgctcgt ctttcaggtt tccagcggaa
     4201 tggttaccgg acgaagccgg tcggccgtat ccaaccgaag taataggcga gcagaatcag
     4261 caggaacagc gcgatcgaga atccgacgcg aaaggcgagg gcggtgaccg tgcggttgct
     4321 gcggcccttg tccttcatca ggaagacgag cgccgaagtc aggctgccga gtatgaaaat
     4381 gaaggcgacg gccacaaagg ctttcattgg aggcgttccc ggcgcggcga gcgcgtggcg
     4441 taagaaagag tgacattgta atggctttga atttccgggt gcggtgggtg ccgctgattg
     4501 cggcgctggc tgccgcttcg gtcggcatcg cgctgggcga ctggcagacg cggcgcgcgc
     4561 aggagaagga ggcgatcgag gccaggttgt ccgtgcgcga gaccgaggcg cccgtacagc
     4621 tttccgccta cgccggcgac ccggccgagc tggaattccg ccgcgtgctg atgcgcggtg
     4681 aattcgtttc ggggtggaac gttttcctcg ataaccggcc ttatgccggg gcggccggat
     4741 tttacgtagc tgcgccgttc cgtctggagc acgacaacag ccacgtgctg gtgctgcgcg
     4801 gctggacgcc gcgcaacatc gcggagcgca cgcgcataca ggaaattccc acgccggacg
     4861 gcagcatcga gatcgagggc atggtgcgaa tgcatatcgg ccggatcatg cagctaggcg
     4921 accccgagcc attgcgtccg ggggcgatcg tgcagaacct cgtgatcgag gaactcgggc
     4981 gcgccggagg ctggaccatg cgaccattcg tggtcgagca gttgagcgac accggagacg
     5041 gtttggtccg cgactggttg cggccctcga ccggcgccga caagcatcgc ggctatgctt
     5101 ttcagtggta tgggctggcg gctgccgccc tggtgttttt tttcgtgaca ggatttcgcc
     5161 gtggaccaaa gtagcaagca gaagaggaac agcggacgct ggaagctgtt cgcagtgata
     5221 gcgatctgtg catcgccgct gatcgcctct tacttcacct attacgtgat caagcccgaa
     5281 gggcgcacca actacggcac cctgcttgat ccgcgcgcgt acccgatgcc ggtactgaaa
     5341 gcgaagacgc tcgacggcat tccggccgaa ctcgagacgc tgcgcggcaa atggatcatg
     5401 ctgcaggttg caccgggagc ctgtgacgac gcgtgccgca agcgcctgca cgacatgcgc
     5461 cagttgcgcc tggcacaggg caagaacatg gaccgggtcg aacgtgtctg gctgatcaac
     5521 gatgccagtc cgctcgagac tctggtcatg cgcgaatacg acggcactca catgctgcgc
     5581 gccgatgcgc aggcgatcgc tgcctggctg ccggccgaaa cgaccaccac ggagagcgac
     5641 cacctgtacg tgatcgaccc gctcggcaat ctgatgatgc ggttcccgaa ggacgccgac
     5701 ccgaaccgga tcaagaagga tttgtcgcgc ctgctcaggg cgtcgagtat cgggtaagcg
     5761 tatgctcgtc caactcgcgc tcaaagggct gctcgccgcg ctgattccgc tactgatcgt
     5821 ctggaccgcg cgcgacagca acaagtaccg aaagctggtc tgggtcacgg ccttcctgac
     5881 cttcgacctg atcatgttcg gcgccttcac gcgcctgacc gactccggcc tcggctgccc
     5941 cgactggccc ggctgctacg gctatgcgaa tccgttccag gcgattgaac acatccgcgc
     6001 tgccgaggcg ctgatgcccg acggcccggt caccgtgatg aaggcctgga tcgagatgat
     6061 ccaccgctac ttcgccatgg gcgttggagt cctgatcatc gccatcatga cgatcgcgtg
     6121 gcgcaactgg ttcagaacgc aacgccagga tgcgcgctat gcgccgggct tcccgacctt
     6181 cctgctgttc ttcgtctgcg tgcagggcgc attcggcgcc tggaccgtca cgatgaagct
     6241 gcagccggtc atcgttacga ttcacctgct gctcggcatg gggctgctcg ccgcgctggt
     6301 ctggctgggc gcgcgccaga acgattatcc gccggtggtc gcggcgcgct cgctgcgccc
     6361 gttggcggcg gccggactcg cggtgctgtt catccagatc ggcctcggcg gctgggtcag
     6421 tacgaattac gcggccctcg cctgcctgga ctatccgctt tgcaacgggc agttcatccc
     6481 cgacatggat ttcgagcacg gcttctcgct ctggcgcgag ctcgggaaga cccgtggcgg
     6541 cgagtacctg ccgttcccgg cactggtcgc catccattgg gtgcaccgca tgtttgcgat
     6601 cgtcgtgctg gctgttctgc tggcgctggc cttgcacgcg cgccgggttg ccgaactgcg
     6661 tcggctggct aatctcttga tgctggccat cggcatccag ttcctgaccg gcatggcgac
     6721 gattttcctg aagtggccgc tggcgcttgc ggtgattcac aacgggggag cggcggtcct
     6781 cgtcgttctg ctcaccgtgc taaactaccg cgctcgcttg gccgtaccgg ccgaggcgca
     6841 acccggcaca acgcggcttt cgcccgcctg atattcattc cgtgaccacg ctgaccgctc
     6901 atccgaaccg cgttgcccag tactgggcgc tgacgaagcc gcgcgtgacc cagcttgcgg
     6961 tcttctgcgc ggtgatcggc atgttcctgg cgagccctgg cctgcccgac tggcgcatcg
     7021 tcgttgccgc aacgatcggg atctggctgc tggccggcgc ggcgttcgcg gtgaactgcc
     7081 tggtcgaacg cgagatcgat tcgcgcatgg cccgcaccgc gcggcggcct gcggcgcgcg
     7141 gcgaaatcac cgtgatgcag atcctggtgt tttccggcgt gatcggcggc gccggcatgt
     7201 gggtgctgtt caatttcgtc aatccgctga cgatgtggct gaccttcgcg accttcgtcg
     7261 gttatgcggt catctacacg atgctgctga agccgatgac accgcagaac atcgtgatcg
     7321 gcggcctgtc cggcgcaatg ccgccggcgc tggggtgggc ggcggtcgcg aacgacgtgc
     7381 cgatggaagc ctggatcctg gtgctgatca tcttcgtctg gaccccgccg catttctggt
     7441 cgctggctct gtaccgccgc gacgactacg cgaagtccgg cttgccgatg ctgccgatca
     7501 cgcacggcct gaagttcacg cagttccaga tctggctgta tacgatcgcg ctggttgcga
     7561 ccacgctgct gccgttcgcg gtgcgcatga gcggcctgat ctatctggtc agcgcgctgg
     7621 tgctgggcgc gatcttcctc tggtacgcat ggcaggtctg gcggcattac tccgacctga
     7681 tcgcccgcaa ggcctttgcc tactcgatcg tctacctgtc gctgctgttc gccgccttgc
     7741 tcgtcgacca ctacctgaag ttctgatatg tcgtattccg ttcgccgtat tgccgccctc
     7801 gcgctgatcg ccggctccgt tctcgtcgcc ggctgcgacc gcctgggtgc aagcggcggc
     7861 acgcagttcc agaacaccga tctcacaggc cttgagtacg cgcgcggatt cgagctgcct
     7921 gaccacaacg ggcagctgcg cacgctggcg gatttccgcg gcaaggcggt ggtcgtgttc
     7981 ttcggcttta cccattgccc ggacgtctgc ccgagcacga tggccgaaat ggcgcaggtg
     8041 atggaacagc tcggtccgga cgccgagagg gtgcaggtgc tgttcgtcag cgtcgacccg
     8101 cagcgcgacc gacccgaaat cctccggcac
//
LOCUS       DATLGF010000275         5350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_180869, whole genome shotgun sequence.
ACCESSION   DATLGF010000275 DATLGF010000000
VERSION     DATLGF010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180869"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="VM406_08440"
     CDS             <1..1102
                     /locus_tag="VM406_08440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HVL76030.1"
                     /translation="DAPPQVTVKIPRASALAYLLTPSLYNLGKAYVEGEIDVVGRSSE
                     IISAGSSLARNALKPEGKFGRIVRTFQHTKKLDEEAIRYHYDVSNDFYRVWLDENMVY
                     SCAYFEYGEEDLATAQMKKIDHILTKIRMQPGQTLLDIGCGWGALVLRAAEKFGARCV
                     GITLSEKQYELASERVREKGLSDRVEIRLQDYRDVTGTFDRITSVGMFEHVGAKNLVP
                     YFSKMRSLLADDGVAMNHGITTTDHGNGETAYGGGEFIHQYVFPHGELQHIGTVLRAM
                     QEGGLEAWDIENLRKHYARTCAIWAENFENNAERVRAEIDDKRFRIWRVYLAGCAYAF
                     SQDWVSLYQVTCTKAGRTPDLMPWSRRYIYAA"
     gene            complement(1127..1528)
                     /locus_tag="VM406_08445"
     CDS             complement(1127..1528)
                     /locus_tag="VM406_08445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HVL76031.1"
                     /translation="MPTIFERLISGELPVAKVYEDELVFAFMDAGQLNPGHVLVATRQ
                     PYETLMDADEESAAAMMRAARRIARAVQQAFNPEGITILQANRPAGWQTVPHLHLHVV
                     PRYANDGVELTWPRKEPGIEVLREYAARIKL"
     gene            complement(1532..2068)
                     /gene="msrA"
                     /locus_tag="VM406_08450"
     CDS             complement(1532..2068)
                     /gene="msrA"
                     /locus_tag="VM406_08450"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HVL76032.1"
                     /translation="MAMESATLGGGCFWCLEAVYQDLKGVEHVESGYAGGHVPNPTYE
                     QVCSGTTGHAEVVRLEFDRQAISYREILEVFFTIHDPTTLDRQGNDVGPQYRSVIFFH
                     TPEQEEIARQVIAEMANVWDAPIVTELAPLPDYYKAEDCHQNYFRNNPMQGYCAFVVA
                     PKVAKFRKVFAKKTKQGG"
     gene            2232..2747
                     /locus_tag="VM406_08455"
     CDS             2232..2747
                     /locus_tag="VM406_08455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HVL76033.1"
                     /translation="MSIPALTAEPPQFDSRQFRDALSQFATGVTVITTRLDNNNFLGL
                     TASSFNSVSLVPPLVLWSLSQGASSLPVFSGNSHYVINVLAGDQMALAERFAMKIENR
                     FEGVDYRLSHTGLPILNGVAAWFECHNRSRYPEGDHVIFVGEVERCEAAPKPALVFHG
                     GRFSSTVPLRK"
     gene            complement(2871..3908)
                     /locus_tag="VM406_08460"
     CDS             complement(2871..3908)
                     /locus_tag="VM406_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HVL76034.1"
                     /translation="MMRMKLAAAFLAAAALAGCGGDRPPQFSSLVSFGDSLSDVGTYA
                     VGTVQALGGGKYTVNGPGPRNWIELMASRLSLAAPCPAQTGLDGDPLQGFSVPVSDAP
                     HCTAYAQGGARVTNPVGPGNKLLGGGNATLGQLTVPVVTQIQRHLTANGGRFRGDEIV
                     FVMAGGNDTFIQLATVAQGGIAPADGVAAMGLAGAQLAGYVRDQLVANGARYVVVVNL
                     PDVSVTPFALAEDAATQGLVNTMVTTFNGQLQQGLSGTDVLLVDAYTTSRDQNANPAR
                     YGISNAIQPACDLSPAVNPLGSSLICTEARLVSGANANYLFADGVHPTPYGYRLLADL
                     VTAEMARRGWM"
     gene            complement(4108..5136)
                     /locus_tag="VM406_08465"
     CDS             complement(4108..5136)
                     /locus_tag="VM406_08465"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HVL76035.1"
                     /translation="MPIVAATPAAIESAAHALEAGELVGFPTETVYGLGADAENPVAV
                     ANIYAAKGRPANHPVIVHVAPGADLGRWAAEVPAEARALVDAFWPGPLTLILKRASHV
                     PDAVSGGQDTIGLRCPSHPVAQALLRAFKGGNGGVAAPSANRFGHVSPTTAEHVRDEF
                     GDGPGSPLACILDGGASEVGIESTIVDLSRIDSHGPVLLRPGGISIAQLEQVLGVPVQ
                     APGSDAPRASGTLEAHYAPGTPVALVDAAELAATLQRLHGNGQRVALLSRSEPRSPYP
                     VFQIDLPPAAEGYAHDLYAALREADEARADLIVVEAPPQDAAWAGVNDRLRRAAHDSR
                     GVLAKLLG"
     gene            complement(5139..>5350)
                     /locus_tag="VM406_08470"
     CDS             complement(5139..>5350)
                     /locus_tag="VM406_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HVL76036.1"
                     /translation="FEPGSEADDGPREPAWDKVLALPGANLHLYGKEEARRGRKMGHV
                     TFVAPTLEAAQENLRAACAILGIAP"
BASE COUNT          905 a         1788 c         1739 g          918 t
ORIGIN      
        1 ggatgcacct ccgcaggtga cggtgaagat tccgcgcgcg tccgccctcg cctacctgct
       61 tacgccatcg ctgtacaacc tcggcaaggc ctatgtcgaa ggcgagatcg acgtggtcgg
      121 ccgctcatcc gaaatcatct ccgccggcag ctcgctcgcc aggaacgcgt tgaagccgga
      181 gggcaagttc ggtcgcatcg tgcgcacctt ccagcacaca aagaagctgg acgaggaagc
      241 gatccgctac cactacgacg tctccaacga tttctaccgc gtctggctgg acgagaacat
      301 ggtctattcc tgcgcctact tcgagtacgg tgaggaagac ctggccaccg cgcagatgaa
      361 gaagatcgac cacatcctca ccaagatccg catgcagcct ggccagaccc tgctcgacat
      421 cggctgcggc tggggcgcgc tggtgctgcg cgctgcagag aagttcggcg cgcgctgcgt
      481 cgggatcacg ctgtcggaaa aacagtacga actggcctcc gaacgggtca gggaaaaggg
      541 tctgtccgac cgcgtcgaga tccgcctgca ggattaccgc gacgtcaccg gcaccttcga
      601 ccgcatcacc agcgtcggca tgttcgagca tgtgggcgcg aagaacctgg tgccctactt
      661 ctcgaagatg cgctcactgc tggccgacga cggcgtggca atgaatcacg gcatcaccac
      721 caccgaccac ggcaacggcg agaccgcgta tggcggcggc gagttcatcc accagtacgt
      781 gttcccgcac ggcgagctgc agcatatcgg caccgtgctg cgggcgatgc aggaaggcgg
      841 actggaggcc tgggatatcg agaacctgcg caagcactac gcgcgcacct gcgccatctg
      901 ggcggagaat ttcgagaaca atgcggagcg ggttcgcgcc gagatcgacg acaagcgttt
      961 tcgcatctgg cgcgtgtacc tcgccggctg cgcgtacgcg ttttcacagg actgggtgtc
     1021 gctgtaccag gtcacctgca cgaaggccgg caggacaccg gacctgatgc cgtggtcgcg
     1081 gcgctacatc tacgcggcgt gacagcacag tcggcacggc cggctttcaa agcttgatcc
     1141 gcgccgcata ctcgcgcagg acctcgatgc cgggctcctt gcgcggccag gtcagctcga
     1201 cgccgtcgtt cgcgtaacgc ggcaccacgt gcaggtgcag gtgcggcacc gtctgccatc
     1261 ccgccggcct gttcgcctgc aggatggtga tgccctccgg gttgaacgcc tgctggaccg
     1321 cgcgggcgat ccggcgcgcc gcgcgcatca ttgccgccgc cgattcctcg tccgcatcca
     1381 tcagcgtctc gtagggctgg cgggtcgcca ccagcacatg gccggggttg agctggccgg
     1441 catccatgaa cgcgaacacc agctcgtcct catacacctt cgcgaccggc agttcgccgc
     1501 tgatcaggcg ctcgaaaatc gtcggcatcg ctcatcctcc ctgtttcgtc ttttttgcga
     1561 aaaccttgcg gaacttcgcc accttcggcg ccaccacgaa agcgcaataa ccctgcatcg
     1621 gattgttgcg gaaatagttc tgatgacagt cctccgcctt gtagtaatca ggcagcggcg
     1681 cgagctcggt cacgatcggc gcgtcccaga cattcgccat ctcggcgatc acctgccgcg
     1741 caatctcctc ctgctccggc gtgtggaaga agatcacgga acggtattgc gggccgacat
     1801 cgttgccctg gcggtcaagc gtggtcggat cgtggatcgt gaagaagact tcgaggatct
     1861 cgcggtagct gatcgcctgg cggtcgaatt ccaggcgtac cacctcggca tggccggtgg
     1921 tgccgctgca cacctgctcg taggtcggat tcggcacatg cccgccggca tagcccgact
     1981 cgacatgctc tacccccttc aggtcctggt acaccgcttc caggcaccag aaacaaccgc
     2041 cgcccagtgt tgccgattcc attgccatca tcgcctcccc gctcatggag cccggcgtcg
     2101 gtggtggtga aacgtctgac ggcgggctgg agcaatgact ttaccgcaaa cagcgggggc
     2161 agtgccttgg ggatcgggtc agggcccttg ctgctttcgc ggcataatgt gcaaaagtct
     2221 ctggatcgtc gatgtctatc cctgctctta ccgccgagcc gccgcagttc gattcccgcc
     2281 agttccgcga tgccctctcg caatttgcga cgggcgtcac cgtcatcacg acgcggctcg
     2341 acaacaacaa cttcctcggc ctgaccgcca gttccttcaa ttcggtctcg ctggttccgc
     2401 cgctggtgct atggagcctg tcgcaaggcg caagcagcct gccggtcttc tcgggcaact
     2461 cgcattacgt gatcaacgtc ctcgcgggcg atcagatggc gctggccgag cgcttcgcga
     2521 tgaagatcga gaaccgtttc gagggcgtcg actaccggct gtcacatacc ggcctgccga
     2581 ttctcaacgg cgtggcggcg tggttcgaat gccataaccg cagccgttac ccggagggcg
     2641 atcatgtcat cttcgtcggg gaagtcgagc gctgcgaagc tgcgccgaag ccggcgctgg
     2701 tgtttcacgg tgggcgattc tcgtccaccg ttccattgcg gaagtgacgc gaccgggata
     2761 gacccggtcg ggatagaccc ggtcgggata gacccggtcg gaatagaccc ggtcggaata
     2821 gacccggtcg gggtagtccc ggtcggggta gacccggtcg ccgcgcagct ttacatccag
     2881 ccccgccttg ccatttccgc cgtgacgaga tccgccagca gccggtaacc gtatggcgtc
     2941 gggtgcacgc cgtcggcgaa caggtagttc gcattggcgc cggagacgag cctggcttcc
     3001 gtgcagatca acgacgaacc gagcgggttg acggccgggc tgaggtcgca ggccggctgg
     3061 atcgcattgc tgatgccata gcgcgccgga ttcgcattct ggtcccggct ggtcgtgtaa
     3121 gcgtcaacca ggagcacgtc cgtaccggac aatccctgct gcagttgccc gttgaacgtg
     3181 gtgaccatgg tgttcaccag tccctgggtt gcggcgtcct cggcgagcgc gaacggcgtc
     3241 acgctcacat ccggcaggtt gacgaccacg acataccgtg cgccgttggc gacgagctgg
     3301 tcccgcacat agcccgcaag ctgggcgccg gccaagccca tggcggccac gccgtcggcc
     3361 ggcgcgatgc cgccttgggc caccgtggcg agctggatga acgtatcgtt gcctccggcc
     3421 atcacgaaca cgatttcgtc gccgcggaaa cggccgccgt tggcggtcag gtgccgctgg
     3481 atctgggtaa ccaccggtac ggtcagctgc ccgagcgtgg cgttgccgcc gccaagcagc
     3541 ttgttgccgg gacccacggg attggtcacc cgcgcgccgc cctgcgcata ggcggtgcaa
     3601 tgcggtgcat cggaaaccgg cacggagaat ccctgcagcg gatcgccgtc caggccggtc
     3661 tgggcggggc aaggcgcagc aagcgaaagg cgcgaggcca tcagttcaat ccagttccgg
     3721 gggcccgggc cgttgaccgt gtacttgccg ccgcccagcg cctgcacggt gccgaccgcg
     3781 taggtaccca cgtcgctcag actgtccccg aacgagacca ggctcgaaaa ctggggcggg
     3841 cggtcgccgc cgcaaccggc caaggcagcc gcggccagga aggcagcggc gagtttcatg
     3901 cgcatcatgt tgtctcctct ctgatcttgt atgcgaacgc acgtgctttt tcgcgcgaca
     3961 agtatgtcac ttcggcaagc ggcccggcta cccgcaggca gcgaaaattg tctgattgat
     4021 tagaggaagg cttctagtgt cctgagtcgc taattcgctg aagaaaagcg gcgaaatcgc
     4081 cgctcaacga actagcgact caggacacta gccgagcagc ttcgcgagca cgccgcggct
     4141 gtcatgggcc gcccgccgca ggcggtcgtt gacgccggcc catgccgcgt cctgcggcgg
     4201 cgcctcgacg acgatcaggt cggcgcgcgc ttcgtcggcc tcgcgcagcg ccgcgtagag
     4261 atcgtgcgca tagccttcgg cggccggggg gagatcgatc tggaagaccg ggtacgggga
     4321 gcgcggctcg ctgcgcgaca gcagggccac gcgctggccg ttcccgtgca ggcgttgcag
     4381 cgtcgccgca agttcggcgg cgtcgaccag cgcaaccggg gtgccgggtg cgtaatgcgc
     4441 ttcgagcgta ccggaagcgc gcggcgcatc gctgccgggc gcctgcaccg gcacgcccag
     4501 cacctgttca agttgcgcga tgctgatccc gccgggacgc aacagcaccg gtccgtggct
     4561 gtcgatgcgc gagagatcga cgatcgtcga ttcgatgccg acctcgctgg cgccgccgtc
     4621 gaggatgcag gcgagcgggc tgccggggcc gtcgccgaac tcgtcgcgca catgctcggc
     4681 ggtggtcggg ctgacatggc cgaagcggtt ggccgacggc gcggccacgc cgccgttgcc
     4741 gcccttgaag gcgcgcagca gcgcctgggc taccggatgc gacggacagc gcaggcctat
     4801 cgtgtcctgc ccgcccgaca ccgcatccgg cacatgggaa gcgcgcttga ggatcaaggt
     4861 cagcggcccc ggccagaacg cgtccaccag cgcgcgcgcc tcggcgggca cttccgccgc
     4921 ccatcggccg aggtccgccc cgggcgcgac gtgcacgatg accgggtgat tcgccggcct
     4981 ccccttggcc gcgtagatgt tcgccactgc caccggattt tcggcgtctg cccccaggcc
     5041 gtacacggtc tcggtcggga agccgaccag ttctcccgcc tcgagcgcat gcgcggcgga
     5101 ttcaatggcg gcaggcgtcg cggcgacgat cggcatggtc agggtgcaat gcccaggatc
     5161 gcgcaggccg cgcgcaggtt ctcctgggca gcctcgagcg tcggcgcgac gaaggtcaca
     5221 tgacccatct tgcgcccgcg ccgcgcctct tccttgccgt acaggtgcag gtttgcgccg
     5281 ggcaaggcaa gtaccttgtc ccaggccggc tcgcgcggcc cgtcatcagc ctcgctgccc
     5341 ggctcgaacc
//
LOCUS       DATLGF010000276         6050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_138343, whole genome shotgun sequence.
ACCESSION   DATLGF010000276 DATLGF010000000
VERSION     DATLGF010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6050
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138343"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VM406_08475"
     CDS             complement(<1..721)
                     /locus_tag="VM406_08475"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVL76037.1"
                     /translation="MDEAYDAVIIGAGPAGSSAAILLARAGWRVALVDKHAFPRNKVC
                     GGCIAASNLPLLNGLGVAAELAMVAGPPIKEVALACGERLIRAPMPRFTGPGSPWGVA
                     LGRDQLDALLAAQARIAGAEVFQPWAAKDTDGVPGAYRCRIRATGTQEERVLVAPVLI
                     DAHGSWEALATRGQRRSVPHRASDLFAFMAQFPGTGLEAGLLPVLSFPGGYGGMVVAG
                     QGATTLAFCLRRDVLAQARTLH"
     gene            complement(728..1453)
                     /locus_tag="VM406_08480"
     CDS             complement(728..1453)
                     /locus_tag="VM406_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVL76038.1"
                     /translation="MSAPTMHVRPAFPRVVSGELLDALDEADPRAVHARKDLIRINRI
                     MGAARILATELAKVEAPPHRIVELGAGDGNLMLQVARRLAPRWKGVQVVLLDRQNLVS
                     PATKQGFAQLGWEVQTLQRDVEDWIADAEGERFDIALANLFIHHFDTRDVRRLLRALS
                     ERTGVFVASEPRRTRTALLASRLVGLIGANDVTRTDAVLSVRAGFRDQELSALWPRND
                     QGWLLDERTAGPFGHLFVARRSE"
     gene            complement(1450..2529)
                     /locus_tag="VM406_08485"
     CDS             complement(1450..2529)
                     /locus_tag="VM406_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HVL76039.1"
                     /translation="MQCDGGLMFINGIGTAVPERRYTKAQCWDAFLASDWFTRLGPRA
                     HALARGVLLRDNGIASRHLALESLAEGFSIDPDTLQSRYLRHAPALAESAAVSAIEQS
                     GLRVGQIDAVVVSSCTGYLCPGLSGYVIERLGLRKDVQAFDLVGQGCAAALPNLRLAS
                     ALLDSGQCRHVLSVCVEVCSAAMYLDEDPGVLISACLFGDGAGAMVLSRCPQEHVRRV
                     EWLHAASLIEPEHREQLMFEQRAGLLRNILSREVPALAAVHAEQVLNQALDQARAARG
                     EIRAWIMHPGGRDVLQALQLQLHLQADELRYSTSVLRDYGNLSSASVYFVMQEALQQD
                     APSGKWWLSSFGAGFSCHGALLEVG"
     gene            complement(2710..3060)
                     /locus_tag="VM406_08490"
     CDS             complement(2710..3060)
                     /locus_tag="VM406_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HVL76040.1"
                     /translation="MAWLITKYLVTAGVVVLVSELAKRSDKLGALIAALPMVTVLALI
                     WLHIEQQPSEKLSNHAWYTFWYVLPTLPMFLAFPALLPRVGFWTALALCVVLTAACFG
                     LLAVLLRYVGIDLA"
     gene            complement(3229..3972)
                     /locus_tag="VM406_08495"
     CDS             complement(3229..3972)
                     /locus_tag="VM406_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVL76041.1"
                     /translation="MAEADTQSVTTIDEIADGIYRIFTPVPPEVMPGGFSFNQYLIAD
                     SRPMLYHTGPRKLFPLVSAAIERVLPVASLAYIGFSHVEADECGALNEFLEVAPDAVP
                     VCSTVAAMVSVDDIALRPAKAMADGEVLDLGRHRVLWIHTPHLPHGWECAMAYEESTR
                     TLLCGDLFTQPGHQHDPVTENDILEPSEAFRRQMDYFAHSPDTRAQLTRLADLKPATL
                     ACMHGSAWRGDAASLLRQLADRVSPSCAS"
     gene            complement(3979..4584)
                     /locus_tag="VM406_08500"
     CDS             complement(3979..4584)
                     /locus_tag="VM406_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVL76042.1"
                     /translation="MAPRQYNSEVRRLAEAQTLRRIVAATVELHKEKGVMASTHAEIA
                     ERAGVSVPTVYKHFPTRHDLLPACMVCVEKDAPAVDPATICAAPDLDGRLTRMVDAIH
                     ARYRYFHPWYRWAPVDAPYLPEVAAAVEAANEELERLVRGVLTKGSGISLSRDKMALV
                     LVLLDYATWLRMSELLVRPKAVSKAVVQALRLVISTREESE"
     gene            complement(4823..5116)
                     /locus_tag="VM406_08505"
     CDS             complement(4823..5116)
                     /locus_tag="VM406_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76043.1"
                     /translation="MFAGEFRSLRAATVPRMADVAAWPWILYPRPTALRQVTNDSFRF
                     WLFMLANMLVGAQQAFAQYYRFAAAGERRLQEPCGVVGDRGQRGRGGRGNQQY"
     gene            5789..>6050
                     /locus_tag="VM406_08510"
     CDS             5789..>6050
                     /locus_tag="VM406_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76044.1"
                     /translation="MKKIYPILASLTLAFLSGCGGSSSDVDVSGGATPSTPAVASVNV
                     QDDPEYIQFLEENSMLYQAARVADPISGSGEQWRREFGVPRPE"
BASE COUNT         1126 a         2023 c         1849 g         1052 t
ORIGIN      
        1 ggtgcagtgt gcgtgcctgc gccagcacgt cgcgccgcag gcagaatgcc agcgtggtcg
       61 cgccctgccc cgccacgacc atcccgccgt acccgcccgg aaaggacagc acaggcagca
      121 ggccggcctc gagtcccgtg cctgggaact gcgccatgaa cgcgaacaga tccgaggccc
      181 ggtgcggcac ggaccggcgt tgcccgcgtg ttgccagcgc ttcccacgac ccatgcgcgt
      241 cgatcagtac cggcgcaacg agcacccttt cttcctgcgt tcctgtcgcg cggatgcggc
      301 aacggtaggc gccgggcacg ccgtccgtgt ctttcgcggc ccatggctgg aacacctctg
      361 ctcctgcgat gcgcgcctgg gccgcaagca gggcatccag ctgatcgcgc ccgagcgcca
      421 cgccccacgg agaaccgggc ccggtgaagc gaggcatggg ggcgcggatg aggcgttcgc
      481 cgcaggcgag cgccacttcc ttgatcggcg gccctgcgac catggcaagt tcggcggcaa
      541 cgccgagccc gttcagcagc gggaggttgc ttgcggcgat gcagccgccg cacaccttgt
      601 ttctggggaa cgcatgcttg tcgaccaggg cgacgcgcca tccggcgcgg gcgagcagaa
      661 tcgccgcgct gcttccggcg ggcccggccc cgatgatcac cgcgtcatat gcctcgtcca
      721 tatccgctca ctcgctgcgt cgcgcgacga acagatggcc gaacggtccg gccgtgcgct
      781 cgtcgagcag ccatccctgg tcgttgcgcg gccagagtgc cgacagttcc tggtcgcgaa
      841 accccgccct cacgctcagc accgcatcgg tgcgggtcac gtcattggcg ccgatcaggc
      901 cgacgaggcg gctcgccagc agcgccgtgc gggtccgcct cggttcgctc gcgacgaaca
      961 cgccggtacg ctcgctcagc gccctcaaca gccgccgcac gtcgcgcgta tcgaagtgat
     1021 ggatgaacag gttcgcgagc gcgatgtcga agcgttcgcc ttcggcatcc gcgatccagt
     1081 cctcgacatc gcgctgcaag gtctgcacct cccacccgag ctgcgcaaag ccctgctttg
     1141 tggcggggct caccagattc tgtctatcca gaagcacaac ctgtacaccc ttccaccggg
     1201 gcgcaaggcg gcgtgcgact tgcagcatca ggtttccgtc ccccgcaccg agctcgacga
     1261 tgcgatgcgg cggcgcctcc accttcgcca actcagttgc cagaatgcgc gccgcgccca
     1321 tgatgcggtt gatgcggatc aggtctttgc gagcatgcac cgcgcgcgga tcggcttcgt
     1381 ccagcgcgtc gagcaactcg cccgagacga cgcgcgggaa ggcgggacgg acgtgcatgg
     1441 ttggcgcgct catccgacct cgagcagcgc gccatggcag ctgaagccgg cgccgaagga
     1501 ggacagccac cacttgcccg agggcgcatc ctgttgcaag gcttcctgca tcacgaagta
     1561 cacggatgcg ctgctgagat tgccatagtc gcgcagcacg ctggtgctgt accggagttc
     1621 gtccgcctgc aggtgcaact gcagctgcag cgcctgcagc acgtcccttc cgcccgggtg
     1681 catgatccag gcgcggattt ccccgcgtgc cgcgcgcgcc tgatcgagcg cctggttcag
     1741 cacctgctcc gcatgcacgg ccgccagcgc cggcacctcg cgcgagagga tgttgcgcag
     1801 cagccctgcg cgctgctcga acatcagctg ctcgcggtgt tccggctcga tcagcgaggc
     1861 ggcgtgcagc cactccaccc ggcgcacatg ctcctgcgga cagcgcgaca gcaccatcgc
     1921 gccggcccca tcgccgaaca ggcaggcgct gatcagcacg cccggatcct cgtccaggta
     1981 catcgccgcg ctgcacacct cgacacatac ggacagcaca tgacggcact ggcccgagtc
     2041 cagcagcgcg ctcgccagcc tcagattggg cagcgcggcc gcgcatccct gcccgaccag
     2101 gtcgaacgcc tgcacgtcct tgcgcagacc gagccgttcg atgacatagc ccgagaggcc
     2161 agggcaaaga tagccggtac aactgctgac gaccactgca tcgatctgtc cgacgcgcag
     2221 tccggattgc tcgatggctg aaaccgcggc gctttcggcc agcgccggcg catggcgcag
     2281 atatcgtgac tgcagcgtat ccgggtcgat gctgaatccc tcggccaggg attcgagagc
     2341 aagatggcgc gacgcgatgc cgttgtcacg cagcagaacg ccgcgcgcga gggcgtgggc
     2401 gcgcgggccg agccgggtga accagtcgga ggcgaggaag gcatcccagc actgcgcctt
     2461 cgtgtagcgc cgttccggta cggctgttcc gatgccgttg atgaacatca ggcctccgtc
     2521 gcattgcacg gtatccggcc gtcatccgac tgccttgcca gtcggcacat gacgggatgc
     2581 atgatcgcgc cgcagattcg tcgcggcgcc tctgctgctt gcagagaacg aatgtcattc
     2641 cgacaaacgg gaagctgcgc ggttcagcgc gaaacggcgc tggctgcacg cggcctgccg
     2701 ctgctcaatt cacgccagat cgatgccgac ataccgaagc agcacggcca gcagcccgaa
     2761 gcaggctgcc gtgaggacga cacacagcgc aagcgcagtc cagaatccga cgcgcggcag
     2821 caacgcagga aaggcaagga acatcggtag cgtcggaagc acgtaccaga acgtgtacca
     2881 cgcgtggttc gacagctttt cgctcggctg ctgttcaatg tgcagccaga tcagcgcgag
     2941 caccgtgacc atgggcagcg ccgcaatcag cgcgcccagc ttgtcgctgc gcttggcgag
     3001 ctccgacacg agcacgacaa cgcctgcggt caccaggtat ttggtaatca gccaggccac
     3061 acgggctcct tccggcggga aatcttcatt tcggaccatc ccgggaaaca atgcgctgtc
     3121 gcaggcgcaa caaggcaacc acctgtcccg atcgccttgc catcggcatg ttggccgggc
     3181 ttgcaggcga aacagcccgc ctcccggccg ttcaggtgag acggccggtc aggaagcaca
     3241 cgacggactc acgcgatcgg caagctggcg cagcagggat gcggcatctc cgcgccacgc
     3301 gcttccgtgc atgcaggcca atgtggccgg cttcaggtcc gcaagccgcg tcagctgcgc
     3361 gcgcgtatcg ggactgtggg cgaagtagtc catctgcctg cggaacgctt cgctgggctc
     3421 gaggatgtcg ttctcggtga ccggatcgtg ctggtgaccg ggttgggtaa agagatcgcc
     3481 gcacagcagc gtgcgtgtcg attcctcata cgccattgcg cattcccacc cgtgcggcag
     3541 gtgcggcgta tggatccaga gcacgcgatg gcggccaaga tccagcacct cgccatcggc
     3601 catcgccttg gccggacgga gcgcgatgtc gtcgacagac accatcgccg ccaccgtgct
     3661 gcataccggc accgcatcag gcgccacctc gaggaactcg ttcaatgcac cgcattcgtc
     3721 cgcttcgaca tgcgaaaacc cgatgtacgc cagagacgct accggcagca cgcgctcgat
     3781 cgctgcggac accagcggaa acagcttgcg cggaccggtg tgatacagca tcggcctgct
     3841 gtcggcgatc aggtactggt tgaacgaaaa cccgcctggc atgacctcgg gcggcaccgg
     3901 ggtgaaaatg cggtagatgc catcggcaat ctcgtcgatg gtggtaaccg actgcgtatc
     3961 agcttcagcc atgattcgtc actcgctctc ttctcgggtt gaaatcacca ggcgcagcgc
     4021 ctggaccact gccttgctca ctgccttggg cctgacgagc agctcgctca tgcgcagcca
     4081 ggtcgcataa tcgagcaaca ccaggaccag ggccatcttg tccctggaaa gagatatgcc
     4141 cgatcccttg gtgagcacgc ccctcactag gcgctccagc tcctcgttgg ccgcctccac
     4201 ggcagccgcg acttcgggca gatacggcgc atcgaccggc gcccaccggt accacgggtg
     4261 gaaatagcga tagcgtgcat ggatcgcatc caccatccgg gtcagccggc catccagatc
     4321 cggcgccgcg caaatggtgg cgggatcgac ggctggcgca tccttctcca cgcacaccat
     4381 gcaggcaggt agcaggtcgt gccgggtcgg gaagtgcttg tagacggtcg gaacggaaac
     4441 gccggcacgc tcggcgatct cggcgtgcgt cgacgccatc acgccttttt ccttgtgcag
     4501 ctcgacggtc gccgcgacga tgcggcgcag ggtctgcgcc tcggcaagcc tgcggacttc
     4561 actgttgtac tgccttggcg ccatttcgtc taactgtcgt ttaattcaat taattctata
     4621 ttaatcggaa tttccgaaag cgcaagcggc atttcgttcg cgagacgcct ggcgattgag
     4681 actttgttca ggcctactcc acgagcacgg ctcgccgccg gaacgttccg cagccagcga
     4741 ttgcagtcaa ggtcagcaaa aggaatggca ctgctgcgac gtttgccgct gcccagccat
     4801 accgattccc tcggtcacgc gctcaatatt gttggttccc gcgaccaccg cgaccacgct
     4861 gcccgcgatc acccacgaca ccgcacggct cttgaaggcg gcgctcgcca gcggcggcga
     4921 aacggtaata ctgcgcgaac gcctgttggg cgccgacaag catgttagcc agcatgaaaa
     4981 gccagaacct gaagctgtcg ttcgtcacct gtctcagcgc ggtcggccgc gggtagagaa
     5041 tccatggcca agcggccaca tccgccatgc ggggaacggt cgctgcgcgc agcgaacgga
     5101 actcgccggc gaacacccac gactgctcgc cggaaaggcg ctagtgtccg acgctgatgg
     5161 cttcctgatg gcagcgtggg cgcagtccat ttcactgcga cgcataatct tgaccggtta
     5221 aaggagatgc gcgaaagatc aaggacggcc ttgcacacaa atatctgcca ccccgcatcg
     5281 ctcgtcactg aaaaagtcgc atgattcatt tctccgcggg ggcagcctta atgaatgctc
     5341 cttcaagtat cgccctttat tggaacctca agttttattc catgaagttg caacgcattc
     5401 tgcgttgtaa tccagcccca tctgcacccg catcgcggtc cacttcggcg ccaaccatac
     5461 gtcgtccttg aacaaaagcc gaacgatttt aataacgcgc tgtctcatca accctctcaa
     5521 tgcaaagttg agcatatgcg gcagtgtccg gattgtggtc ggcttgaacg caccagagat
     5581 ccggcttaac ggcaccgtgc aagcggcggc gactactcat gctgttcctc cttttctagt
     5641 caattcttcg ctttcgcatg caatggcggg gtgttaaacc gcgcccggat ccgatgcttc
     5701 ccttattcca cttccctccc taaagctggt accggtgttc cagtcaccgg agtcaaccga
     5761 tgccggcaaa tttatcttgg aaaaaatcat gaagaaaatt tatccgattc ttgcctcgtt
     5821 gacgttggcg ttcttaagcg gttgcggcgg aagttcgagt gatgtggacg tgagcggggg
     5881 cgcaacgccg tccacgcccg ccgttgcgtc cgtgaacgtc caggacgatc ccgagtacat
     5941 ccagtttttg gaagagaatt ccatgttgta ccaggccgca cgcgtggcgg atcctatttc
     6001 cggcagcggc gaacaatggc gccgcgagtt cggcgtgccc aggccggaag
//
LOCUS       DATLGF010000277         5241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_180895, whole genome shotgun sequence.
ACCESSION   DATLGF010000277 DATLGF010000000
VERSION     DATLGF010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180895"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VM406_08515"
     CDS             complement(<1..318)
                     /locus_tag="VM406_08515"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HVL76045.1"
                     /translation="MKNTKLIVALFAAAFAAGCGTVGQQGAAPAGAQPQAQAPVVDDA
                     VVTSRVQAALAADPEVSPIKINVSTNAGRVTLKGEIKSMALRKRVESIVRGVEGVRSL
                     DNQL"
     gene            complement(813..1442)
                     /locus_tag="VM406_08520"
     CDS             complement(813..1442)
                     /locus_tag="VM406_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL76046.1"
                     /translation="MRIESEHGVWEAALWMPAEPAGAVVFASENGSHRLRPSGDYLPG
                     VLREARLATLVFDAVAPAAAARRAGGVEGEAVGACLHAACEWLSRHPAAADLPLGLAG
                     VGRGATAALQVASERGRTVSAVVARGSCAQRGELPGIAGVAAPTLLIAGGLDDPAVAC
                     NRRAYAALRCKKRFEIVPGATAAFDEPGSLEVVARLARNWFLQYLYQYA"
     gene            complement(1630..1863)
                     /locus_tag="VM406_08525"
     CDS             complement(1630..1863)
                     /locus_tag="VM406_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76047.1"
                     /translation="MEPERNKPPKPSRRIRRRRISMVLVAAVLAVSSCGRHDEGPLPP
                     HSPPRPTTLYGGELTHAHFHLPARPSPPLRGMM"
     gene            complement(1879..2862)
                     /locus_tag="VM406_08530"
     CDS             complement(1879..2862)
                     /locus_tag="VM406_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HVL76048.1"
                     /translation="MRAAAARILVTGGAGFLGSHLCEALLARGAAVVCVDNFHTGRRD
                     NVAHLLAHPRFELIEHDICEPLQLEVDAIYNLACPASPPHYQKDPVYTTRTCVLGALN
                     MLELARRHGVPILQASTSEVYGDPAVHPQPESYWGHVNPVGVRSCYDEGKRCAESLFM
                     DYRRRHAVPVKIARIFNTYGPRMDPEDGRVVSNFIVQALAGAPLTIYGDGLQTRSFCH
                     VDDLIEGLMRLMAAPSTFCGPVNLGNPAETTMRELAARVLALTGSSSTLVHRDLPADD
                     PARRCPDIGLARRVLDWSPRVELDDGLAGTISWFRKRVCPGQERRLHVASR"
     gene            complement(2859..4598)
                     /locus_tag="VM406_08535"
     CDS             complement(2859..4598)
                     /locus_tag="VM406_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HVL76049.1"
                     /translation="MYTLGINAAYHDCAACLVENGQVVAAAEEERFTRIKHGKRPVPF
                     STWQLPYHAIDYCLGEAGIALADVDHVAYSYQPSLLLGEHDGKASITLPLEPSAHNGG
                     EWMSPWDPLFLSSIVNAPRQLASGAPHHLRKRFAGVRHDGPFRWHFVEHHLAHEASAF
                     LAAPFEECAVLTMDGRGERATTSYGLFRDRRYKRLKQVNLPHSLGLLYEALTTHLGFL
                     HSSDEYKVMALASYGKPVFAEQFREILKYRGDGEYTVRPMDARDWEERFGPARERGAA
                     FTEHHMNVASSLQIVLEETVLQMADWLHAQTGADTLCMAGGVALNCVMNARVRDRGPF
                     RNVWVQPAAGDAGTALGAALWIDAQARGPGQSRWTMEHAFLGPAFSDDEIEGVLRQAK
                     LHYRRAGDLSTEVVDLLVRNKIIGWFQGRMEFGPRALGARSILASPLDASMQQRLNTI
                     KDREDFRPVAPVVLEDKAAEWFVDGAVSPFMTFVYDVRSDKADRIPAVRHVDGTARIQ
                     TIRRDQNPAYHDLLQAFAARTGVPVLINTSFNTRGEPVVCTPRDALESFYTTPLDALA
                     IGPFIIEKEQGAA"
     gene            complement(4628..4933)
                     /locus_tag="VM406_08540"
     CDS             complement(4628..4933)
                     /locus_tag="VM406_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76050.1"
                     /translation="MSAEVIGWASAFILVLTISRQVYTQYRTRSIAGVSKWLFIGQLS
                     ASVGFTIYSWLLNNWVFVVVNFSLFLTAVVGQFIYLRNKRHAQREGKGGLQAGENAT"
     gene            complement(4930..>5241)
                     /locus_tag="VM406_08545"
     CDS             complement(4930..>5241)
                     /locus_tag="VM406_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HVL76051.1"
                     /translation="QKFIICAELPGVSLDDVNVEIKHDRLTIEGDRRPEPQHEPDEQR
                     RSERSYGHFYRVVALPPGAQPEGASASMQDGLLEITVPLSAHPYQTRRIDVRPKQERN
                     A"
BASE COUNT          902 a         1744 c         1703 g          892 t
ORIGIN      
        1 cagctgattg tccagcgagc gcacgccttc gacaccgcgc acgatcgact cgacgcgctt
       61 gcgcagcgcc atgctcttga tctcgccttt cagggtcacg cggccggcgt tcgtgctcac
      121 gttgatcttg atcggggaga cttccgggtc cgcggccagc gcagcctgca cacgggaggt
      181 gacgacggcg tcgtcgacta ccggagcctg ggcctggggt tgcgcgccgg ccggagccgc
      241 gccttgctgg ccgactgtgc cgcagccggc ggcgaaggcg gctgcgaaca gggcgacgat
      301 gagtttggtg ttcttcattg ttggtccttg atgaaaatct tctgactgga tcaggggttg
      361 cacgcggcgc gtacagcgtg cgccgcctgc cggccgatta tagtgaaacc gtcatctttc
      421 caaattgcga tagcgcttgt ggatttcact tagaagccct gacaaaatgc cgatggcaag
      481 gcgcgccgac gcagacagta cgtgtagtac ggcaaggagg cgcaacgccg ctcacggctt
      541 tttgttaggg cttcgtagca cgtcatggca tgttcgcgca gcagtccggt gccggatacg
      601 ctagcgcaaa ccctgcagca agtggctttg cagtcggaag ggccgttcag tcaagcgaac
      661 aacgtcggca tggcgtcctc gcggacgcgc tgggcccgca cattcaattg cttgtcgtac
      721 tgcttgcggc cgagccggcc tcctgccttc gggggcagca tgcctggaga tttgcgcctt
      781 actgaacggt gtcccttgca ctcaaccatt tcctatgcgt actgatacag gtactgcagg
      841 aaccagttgc gcgccagacg cgcaaccacc tccaggctgc ccggctcgtc gaacgccgcc
      901 gtggcgcctg gcacgatttc gaagcgcttc ttgcagcgca gggccgcgta ggcgcggcga
      961 ttgcaggcca ccgccggatc gtccaggcct cccgcgatca gcagcgtcgg cgcggcgacg
     1021 ccggcaattc ccggcagttc accgcgctgc gcacaactgc cgcgcgccac gacagcactc
     1081 accgtcctgc cgcgttcgga tgcaacctgc aaggctgccg tcgcgccccg accgacgccc
     1141 gccagaccaa gcggaaggtc ggccgccgcc gggtggcggc tcagccattc gcaggctgcg
     1201 tgcaggcaag ccccgaccgc ctcaccttcc acgccgcccg cgcgccgcgc cgccgccgct
     1261 ggcgcgaccg cgtcgaacac gagcgtggca aggcgcgcct cgcgcaggac accgggaaga
     1321 tagtcgccgg aagggcgcag ccgatggctg ccgttctcgc tcgcaaacac gacggcaccg
     1381 gccggctccg ccggcatcca cagcgcggcc tcccatacgc catgctccga ttcgattctc
     1441 accccttccc ttcgtatcat tgactccatc ttccttcctc tccatcggac cgcgccatta
     1501 cagcgctcgc cggtcatccc cggacagcgc gcgtggggtc gaccgactca gcgcttcatg
     1561 ccacgaggcc gcaccttgac ggccggtcgc catggatcgc aaggtccatg ccagcgccgt
     1621 tacatgccgt tacatcatcc cgcgcagcgg cgggctcggc ctggcaggaa ggtgaaagtg
     1681 ggcatgagtg agttctccgc cgtacaaggt ggtcgggcgc ggcggagagt gcggcggcag
     1741 cggcccttcg tcatgccgcc cgcaggacga gacggccagc accgcggcca ccagcaccat
     1801 cgagatccgg cgtcggcgga tcctcctgga aggcttcggt ggcttgttgc gctctggttc
     1861 catggcgaca cctcgtagtc aacgactggc gacatgcaag cggcgttcct gaccggggca
     1921 cacgcgcttt ctgaaccagg agatcgtgcc tgcaagcccg tcgtccagct cgacgcgcgg
     1981 cgaccagtcg agcacccggc gcgcaagacc gatgtcggga cagcgccgcg ccggatcgtc
     2041 ggccggcaga tcgcgatgga cgagagtcga ggaagacccg gtcaacgcga gcacgcgcgc
     2101 ggccagttca cgcatcgtcg tttcagcggg attgccgaga ttgaccggtc cgcagaaggt
     2161 tgacggggca gccatcagcc gcatcagacc ttcgatcaga tcgtccacgt gacagaacga
     2221 acgcgtctgc agtccgtcgc cgtagatcgt gagcggcgcg ccggcaagtg cctggacgat
     2281 gaagttcgac accacgcgcc cgtcttccgg atccatgcgc gggccgtagg tgttgaagat
     2341 acgtgcaatc tttaccggca ctgcatgacg ccggcggtag tccatgaaca ggctctccgc
     2401 acagcgcttg ccctcgtcgt aacaggagcg cacaccgacc ggattgacat gaccccaata
     2461 ggactccggc tgcggatgca ccgccggatc gccgtagact tcgctggtcg aggcctgcag
     2521 gatcggcacg ccgtgccgcc gcgccagctc cagcatgttg agcgcgccca gcacgcaggt
     2581 gcgggtggtg tagaccgggt ccttctgata gtgcggcggg gaggcggggc aggccaggtt
     2641 gtagatggca tccacttcga gctggagcgg ttcgcagatg tcgtgttcga tcagttcgaa
     2701 ccggggatgc gcaagcaggt gcgcgacgtt gtcgcgccgt ccggtatgaa aattgtcgac
     2761 acacaccact gcggcgccgc gcgcgagtag cgcctcgcac agatgcgatc cgagaaaacc
     2821 ggcaccgccg gtcaccagga tgcgcgcagc ggcagctctc atgcggcgcc ctgctctttc
     2881 tcgatgatga atgggccgat cgccagcgcg tccaggggcg tggtgtagaa ggattcgagc
     2941 gcatcgcgtg gcgtgcacac caccggttcg ccgcgcgtgt tgaacgaggt gttgatcagt
     3001 accggcacgc cggtgcgcgc ggcgaacgcc tgcagcagat cgtggtaggc cggattctgg
     3061 tcgcggcgta tggtctggat gcgtgccgtc ccgtcgacat ggcgcaccgc cggaatacgg
     3121 tctgccttgt cgctgcgcac gtcatacacg aaggtcatga aaggcgagac cgcgccatcg
     3181 acgaaccact cggccgcctt gtcctccagc accaccggcg cgaccggccg gaagtcctcg
     3241 cggtccttga tcgtgttcag gcgctgctgc atcgaggcat cgagcggcga ggcgaggatg
     3301 gatcgcgcgc cgagcgcgcg cgggccgaac tccatgcgtc cctggaacca gccgatgatc
     3361 ttgttgcgca cgagcagatc caccacctcg gtcgacagat cccctgcgcg ccggtagtgc
     3421 agcttcgcct ggcgcagcac gccttcgatc tcatcgtcgg aaaatgccgg gccgaggaaa
     3481 gcgtgttcca tggtccagcg cgactgaccc ggcccgcgcg cctgtgcatc gatccacagt
     3541 gccgcaccga gcgcggtgcc ggcatcgccg gccgccggct gcacccatac attacggaac
     3601 ggcccgcggt cgcgcacccg cgcgttcatc acgcagttga gcgccacgcc gcccgccatg
     3661 cacagcgtgt cggcgccggt ttgcgcatgc agccagtcgg ccatctgcag caccgtctcc
     3721 tccagcacga tctgcagcga cgaagcgacg ttcatgtgat gctcggtgaa cgcggcgccg
     3781 cgctcgcgcg ccgggccgaa ccgctcttcc cagtcgcgcg cgtccatggg cctgaccgtg
     3841 tattcaccgt cgccgcggta cttcaggatc tcgcggaact gctcggcgaa aaccggcttg
     3901 ccgtaggagg ccagcgccat caccttgtac tcgtcggacg aatgcaggaa gcccaggtgc
     3961 gtggtgagcg cttcatagag caagccgagc gagtgcggaa gattgacctg cttgaggcgc
     4021 ttgtagcgcc ggtcgcggaa cagcccgtag ctggtggtgg cgcgctcgcc gcgtccgtcc
     4081 atcgtcagta ccgcgcactc ctcgaacggc gcggcgagaa acgcactggc ctcgtgggcc
     4141 aggtgatgct cgacaaaatg ccagcggaac ggaccgtcat ggcgcacccc ggcaaaacgc
     4201 ttacgcagat gatgcggagc gcccgaagcg agctggcgcg gcgcattcac gatggaagac
     4261 aggaacagcg gatcccaagg cgacatccac tcgccgccgt tgtgtgccga gggctccagc
     4321 ggcagcgtga tggaagcctt gccgtcgtgc tcgcccagca gcagcgaggg ctggtaggaa
     4381 taggcgacgt gatcgacgtc ggcaagcgcg atgcccgcct cccccaggca gtaatcgatc
     4441 gcgtgatacg gcagctgcca ggtcgagaac ggcaccggac gcttgccgtg cttgatgcgc
     4501 gtgaagcgtt cttcttcggc agccgccacc acctggccgt tctcgaccag gcaggcggcg
     4561 cagtcgtggt aggcggcatt gattcccagc gtgtacacgt cttcctccca ttgttgtcag
     4621 cctgcgttca tgtcgcgttc tctcccgcct gcagaccgcc cttgccctcg cgctgcgcat
     4681 gccgcttgtt gcgcaggtag atgaactggc caacgacggc ggtcaggaac agcgagaaat
     4741 tgacgaccac gaacacccag ttattcagca gccagctgta gatcgtgaag ccgaccgatg
     4801 ccgacagctg gccgatgaac agccacttcg agacacctgc gatgctgcgt gtgcggtact
     4861 gcgtataaac ctgccgggag atggtcagga cgaggatgaa ggcgctggcc caaccgatca
     4921 cttcggcact catgcgttcc tttcctgttt ggggcgcaca tcgatgcgcc gggtctgata
     4981 ggggtgagcc gaaagcggca ccgtgatttc aagcaggcca tcctgcatcg aggccgatgc
     5041 gccctccggc tgggcgccgg gcggcagcgc gacgacccgg tagaaatgcc cgtagctgcg
     5101 ctcgcttctg cgttgctcgt cgggctcgtg ctgcggctcg ggccggcgat cgccttcgat
     5161 ggtcagccgg tcgtgcttga tctcgacatt gacatcgtcc agcgacacgc cgggcagttc
     5221 cgcgcagatg atgaatttct g
//
LOCUS       DATLGF010000278         5760 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_44145,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000278 DATLGF010000000
VERSION     DATLGF010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5760
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44145"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..1086
                     /locus_tag="VM406_08550"
     CDS             220..1086
                     /locus_tag="VM406_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVL76052.1"
                     /translation="MTVVDGVSFNLRRGECYGLLGPNGAGKTTTLRLCLGLTEPDGGE
                     IRLVGRPIPAEARAARMRVGVVPQGDNLDPDFTVAENLLVFGRYFGMEDATIEARIPK
                     LLEFANLAAKKDARISELSGGMKRRLTLARALVNDPDIIFMDEPTTGLDPQARHMIWD
                     RLKTLLAEGKTIVLTTHFMDEAERLCDRLAVIDHGRLIAEGNPRDLIAQHIEAEVIEV
                     YGDNALVWGESVGRAAAARMEVSGETVFCYVDDAQPLLATLSNVPGVRYLHRPANLED
                     LFLKLTGREMRD"
     gene            1091..1873
                     /locus_tag="VM406_08555"
     CDS             1091..1873
                     /locus_tag="VM406_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL76053.1"
                     /translation="MARLYAPPAFSLRFVPIWRRNFLVWRKLAGPSLLGNIAEPLITL
                     IAFGYGLGSLLQQIDGVPYIEYLASGAICMSVMMAASFESLYSAFSRMHVQKTWEALL
                     NAPLGLDDVVLAEMLWAATKAIVSGVAILGVILALGIGHHPQAVLVLPILFLIGMTFA
                     SLGLIMNALARSYDFFTYYFTLLLTPMIFVSGIYYPTAQLPDWLQSLAHIMPLGAAVN
                     LVRPLLLGGWPADPWADLAVLVAYCAAAFYMATIVTRRRLLK"
     gene            1879..2211
                     /gene="cutA"
                     /locus_tag="VM406_08560"
     CDS             1879..2211
                     /gene="cutA"
                     /locus_tag="VM406_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HVL76054.1"
                     /translation="MDNALLVITNVPDEAIGETIARSVVEQRLAACVNLLPKIRSVYR
                     WQDAIEEAGEHTLLVKTAASRYAELEAAIKALHPYEVPEIIAIPIQAGLPAYLEWVMQ
                     ETKKNVDI"
     gene            2234..4006
                     /gene="dsbD"
                     /locus_tag="VM406_08565"
     CDS             2234..4006
                     /gene="dsbD"
                     /locus_tag="VM406_08565"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HVL76055.1"
                     /translation="MNEWARIPVLFLLGLALLWPMSAKAEDFLEPEKAFRFSARMADP
                     QTAEIVFRIADGYYMYRERFAFKADGARLGEAIIPEGKIKFDETFQKDVETHRGTLSI
                     RVPVEAAGPFTLHVTSQGCSDKGLCYSPMDSKVRLSPTGGGGTLAALLGTSQPPLLQS
                     VPGMSPAIAPQAEMGRIEASLASGQLMVIVPLFLLLGLGLAFTPCVLPMVPILSSIII
                     GEGRHVSRGRGLALAASYSLGMALVYTGLGVAAGLAGEGLAASLQTPLALGVFALLMV
                     LLSLSMFGVYQLQVPAALQAKLMRVSDRQPAGKLAGVFGMGAISALIVGPCVAAPLAG
                     ALMYISQTRDVMIGGSALFAMAAGMSVPLLLVGVSAGALLPRAGAWMTAVRILFGVLM
                     LALAVWILAPVVPAWFTMAGWAALAIGYGIFLLRGRFGWFSKLAALLLLGLGLVQVAG
                     LATGGRDALAPLAHLGGSAHKTEFQRVRSVAELDAVLARADGRTVMLDFYADWCVSCI
                     EMEKLTFSDPRIKAEFADMVLLQADVTANNEDDKALLRRFRLFGPPGIIFFDGQGREI
                     PGGRVIGFQDARRFGETLELVKGF"
     gene            complement(4023..5129)
                     /locus_tag="VM406_08570"
     CDS             complement(4023..5129)
                     /locus_tag="VM406_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HVL76056.1"
                     /translation="MEVFHLTGSSVALLSEADASIPANGGFLWIDATREEVAADTPAW
                     RTTVERLTGVRLYEPHLQDILNAAHPSYFDPTHAYAMLVFRKLAIDESAAAEPDTPHR
                     KRRIPPALTRLSTKPVTFMLMDRALVTVRPAHSRTLAAFRTRLLEHKPRPENGLYGMR
                     LPASPPELALRLLNALVDQYLALRDPLTGQIERWQRALLSPRSRFNNWSALLDARLEL
                     RKLDQLCEGQRDAVQALRDYLMDEGNGPEDARSRDLLRVRANDVMEHITRVLTHTQRL
                     EAGIESAVQIHFSAMAHRTSEVMRILTVITAIFMPLTLITGVFGMNFEALPLLDHPAG
                     FWISLGMMAATVAVLLALFWRKRYLEDQVVDHER"
     gene            complement(5137..>5760)
                     /gene="hemB"
                     /locus_tag="VM406_08575"
     CDS             complement(5137..>5760)
                     /gene="hemB"
                     /locus_tag="VM406_08575"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HVL76057.1"
                     /translation="DPYTSHGQDGVINHDGYVLNDETTSILVRQAILQAEAGVDIVAP
                     SDMMDGRVGAIRAGLEARHMVHTRIMAYSAKYASAYYGPFRDAVGSAANLGKGDKSTY
                     QMDPANGDEALREVALDLAEGADMVMVKPGMPYLDVVRRVKEEFRVPTFAYQVSGEYA
                     MIKAAAQNGWVDHDKVMMESMLCFKRAGADGVLTYFARDVARRLKQS"
BASE COUNT          947 a         1835 c         1867 g         1111 t
ORIGIN      
        1 gatcagcgat gaggcggaag tgccggcagc cgaataagct acctgtcccc cgtgcatgaa
       61 aagccaggct ccgcctggct tttttgcttg agcggctcga tgaaccggac cctccgatat
      121 ggcaagatga gcttttctcc agtcccgcct cgcatgaccg agcacgctat tctcgccgtt
      181 tcgaacctgc gcaaatccta tggaaatggc gacgcgcgca tgaccgtggt ggacggtgtg
      241 tcgttcaatt tacggcgcgg cgaatgctat ggcttgctcg gcccgaacgg agcaggcaag
      301 accacgacgc tgcgcctgtg cctcgggctc acggaacctg acggcggcga gatccggctt
      361 gtaggccggc cgattcccgc cgaagcccgg gccgcgcgca tgcgcgtggg cgtcgtacct
      421 cagggagaca atctcgatcc ggacttcacc gtcgccgaga acctgctggt cttcggacgc
      481 tacttcggga tggaggatgc aacgatcgaa gcacgcattc ccaagttgct cgagtttgcg
      541 aacctcgccg cgaagaagga cgcgcgcatt tccgagcttt ccgggggcat gaagcggcgc
      601 ctgacgctgg cgcgggcgct cgtgaacgac ccggatatca tattcatgga cgagccgacc
      661 accggcctcg atccgcaggc ccggcacatg atctgggacc gtctgaagac tttgctcgcc
      721 gaaggcaaga cgatcgtgct gacgacacac ttcatggacg aggccgagcg cctgtgcgac
      781 cgcctggcgg tgatcgatca cggccgcctg attgccgagg gaaatccgcg cgacctgatt
      841 gctcagcaca tcgaagcgga agtgatcgaa gtgtatggcg acaatgcgct ggtctggggg
      901 gagagcgtgg gacgtgcggc tgctgcgcgg atggaagtga gcggcgagac cgtcttctgc
      961 tacgtcgatg atgcgcagcc gctgctggcg acgttgagca atgtgcccgg cgtacgctac
     1021 ctgcatcggc cggcgaacct tgaagatctg ttcctgaaac tcaccggccg cgagatgcgc
     1081 gactgaaacc atggcccgcc tgtacgcccc tccagccttc tcgctgcgct tcgtgccgat
     1141 ctggcgccgc aatttccttg tctggcgcaa gctggccggg cccagcctgc tcggcaacat
     1201 cgcggaaccc ttgattacgc tgatcgcatt cggctacggg ctcggcagcc tgctgcagca
     1261 gatcgacggc gtgccctata tcgagtacct tgcgtccggc gcgatctgca tgtcggtgat
     1321 gatggccgcc tcgttcgaat cgctgtattc ggcgttctcg cgcatgcacg tgcagaaaac
     1381 ctgggaagca ttgctgaacg cgccgctcgg actggatgac gtggtgcttg ccgagatgct
     1441 gtgggcggcc accaaggcga ttgtctccgg cgtggcgatc ctgggcgtga tcctcgcgct
     1501 cggtatcggc catcatccgc aggccgtgct ggtgctcccg atcctgttcc tgatcgggat
     1561 gaccttcgcc tcactgggcc tcatcatgaa tgccctggcg cgcagttacg atttcttcac
     1621 ctactacttc accctgctgc ttaccccgat gatcttcgta tccgggattt actacccgac
     1681 cgcgcagctg ccggattggc tgcagtcact cgcccatatc atgccgctcg gggcagccgt
     1741 caacctcgtg cgcccgctgc tactcggcgg ctggccggcc gacccgtggg ccgatctcgc
     1801 ggtattggtc gcctattgcg cggcggcctt ctacatggcg accattgtca cccggcgccg
     1861 cctattaaaa tgacgcgcat ggataacgct cttcttgtca tcacgaatgt ccccgatgaa
     1921 gccatcgggg agaccattgc ccgcagcgtg gtcgaacagc ggctcgcagc ctgcgtgaac
     1981 ctattgccga aaatcaggtc tgtttaccgt tggcaagatg ccattgagga agccggcgag
     2041 cataccttgc tggtaaagac cgcggcaagc cgctacgcag agctcgaggc ggcgatcaag
     2101 gcactccatc cgtacgaggt cccggagatt attgccattc ctatccaggc cggcctgccg
     2161 gcttatctgg aatgggtcat gcaggaaacg aaaaagaatg tcgacatata acttggcgga
     2221 acgcgcgcgg gacatgaacg aatgggcgag gattcctgtc ctgttcctgc tcgggctcgc
     2281 tctgctgtgg cccatgagcg cgaaggccga ggattttctc gagcctgaga aggcgttccg
     2341 cttctcggcg cgcatggccg acccccagac cgcggaaatc gtgttccgga tcgccgacgg
     2401 ctactacatg taccgcgagc gcttcgcgtt caaggcggac ggggcccgtc tcggcgaagc
     2461 gatcatcccg gaaggcaaga taaagttcga cgagactttt caaaaggacg ttgaaaccca
     2521 ccgcggcacg ctctcgatac gcgtcccggt ggaggccgcc ggaccattca cgctgcacgt
     2581 gaccagccag ggctgttcgg acaagggcct gtgctactcg cctatggact ccaaggtccg
     2641 cctgtctccg accgggggcg gcggcacgct ggcggcgctg ctcggcacgt ctcagccgcc
     2701 gctgctgcaa tcggttcccg gcatgagccc ggctatcgcg ccccaggcgg aaatgggccg
     2761 tatcgaagcc tcgctcgcca gcggccagct gatggtcatc gttccgctgt tccttctgct
     2821 cggcctcggt cttgcgttta cgccctgcgt gctgccgatg gtgcctatcc tgtcctcgat
     2881 catcatcggg gaagggcgcc acgtaagccg gggtcgggga ctcgccttgg ccgcctccta
     2941 ttccctcggc atggcgctgg tatacaccgg ccttggcgtc gccgccggcc tggccgggga
     3001 ggggctggcc gcgtcgctgc aaacaccatt ggccctgggc gtgttcgcct tgctgatggt
     3061 gctgttgtcg ctgtcgatgt tcggcgtcta ccaattgcag gtgccggcgg cgctgcaggc
     3121 aaagttgatg cgcgtctccg accgccagcc ggccggcaag ttggctggcg tgtttggcat
     3181 gggtgcgatc tcggccctga tcgtcggtcc gtgcgtggcg gcgccattgg ccggcgcgct
     3241 gatgtacatc agccagacac gcgacgtgat gatcggtgga tcggcgctgt tcgcaatggc
     3301 ggccggcatg agcgtgccgc tcctgctcgt cggtgtgtcg gccggcgcac tgctgccgcg
     3361 cgccggcgcc tggatgaccg ccgttcgcat cctgttcggc gtgctgatgc tggcgctggc
     3421 cgtctggatt ctcgcccccg tggtgcctgc gtggttcacg atggcaggct gggcggcgct
     3481 ggccatcggt tacggcatct tcctgctgcg cggcagattc ggctggttct ccaagcttgc
     3541 ggcgctgttg cttctgggtc tgggcctggt gcaggtggca gggctggcca caggcggccg
     3601 cgatgcgctc gctccgctcg cgcatcttgg cggaagtgcc cacaagaccg agttccagcg
     3661 cgtgcgctcg gtggccgaac tcgacgctgt cctcgcgcgc gccgacggcc gcaccgtgat
     3721 gctggatttc tacgccgact ggtgcgtgtc atgcatcgaa atggaaaagc tgaccttcag
     3781 cgatccgcgc atcaaggcag aattcgccga catggtgctg ctgcaggccg acgtcaccgc
     3841 aaataacgag gacgacaagg cgctgctgcg ccgattccgc ctgttcggcc cgccgggcat
     3901 catctttttc gacggccaag gccgcgaaat tccgggcggc cgcgtcatcg gcttccagga
     3961 tgcgcggcgc ttcggcgaaa cgctcgagct ggtgaagggc ttttagcgct gttgaaggat
     4021 cttcagcgct cgtgatcgac gacctggtct tccaggtagc gtttgcgcca gaacagcgcg
     4081 agcagcaccg ctacggttgc cgccatcatc ccaagcgaga tccagaatcc ggccggatgg
     4141 tccaaaagcg gcagggcctc gaagttcatg ccgaacacgc cggtgatcag ggtcagcggc
     4201 atgaagatcg ccgtgatgac ggtcaggatc cgcatgactt cgctggtgcg atgcgccatc
     4261 gccgagaagt ggatctgcac ggccgattcg atgccggcct cgaggcgctg ggtatgcgta
     4321 agcacccggg tgatatgttc catcacgtcg ttcgctcgca cgcgcagcag atcgcgcgag
     4381 cgcgcatcct cggggccgtt tccttcgtcc atcagatagt cccgcagcgc ctggaccgcg
     4441 tcacgctggc cttcgcagag ctggtccagc ttgcgcagtt ccaggcgggc atcgagcaag
     4501 gcgctccagt tattgaaccg gctgcgcgga ctcagcagcg cgcgctgcca gcgttcgatc
     4561 tgaccggtca gcggatcgcg cagggccagg tactgatcga ccagcgcatt gagcaggcgc
     4621 agcgccagtt cgggcggcga cgccggcagg cgcattccgt acagcccgtt ttccggccgc
     4681 ggcttgtgct cgagcaggcg agtgcggaag gcggcgagcg tcctgctgtg cgccggcctc
     4741 accgtgacca gcgcgcggtc catcagcatg aaggtcaccg gcttggtcga cagccgtgtc
     4801 agcgcgggtg gaatacgtcg cttcctgtgc ggcgtgtcgg gctccgccgc ggccgactcg
     4861 tcgatggcga gcttgcggaa caccagcatc gcgtaggcat gcgtcgggtc gaaatacgaa
     4921 gggtgggccg cgttgaggat gtcctgaaga tggggttcat acaggcgcac gccggtcagc
     4981 ctctcgaccg tcgtccgcca ggccggggta tcggccgcaa cttcttcgcg cgttgcatcg
     5041 atccagagaa agccgccgtt ggccggaatc gacgcatcgg cttcggacag cagcgcgacg
     5101 ctactacccg tcaggtgaaa cacttccatg ccccgcctag ctctgtttca gccggcgcgc
     5161 gacatcgcgg gcgaagtaag tgagcacgcc gtctgcaccg gcgcgcttga agcagagcat
     5221 cgattccatc atcaccttgt cgtggtccac ccagccattc tgggcggcgg ccttgatcat
     5281 cgcgtactcg ccgctgacct ggtaggcgaa agtcggtacc ctgaactcct ccttgacccg
     5341 gcgcaccacg tccaggtacg gcatcccagg cttgaccatc accatgtcgg cgccctcggc
     5401 cagatcgagc gcaacttccc gcagcgcctc gtcgccgttg gccgggtcca tctggtaggt
     5461 cgacttatcg cccttgccca gattcgcggc agagccgacg gcgtcgcgga aggggccgta
     5521 atacgcggac gcatacttgg ccgaataggc catgatgcgg gtgtggacca tgtggcgagc
     5581 ttcgagaccg gcacggatcg cgccgacgcg gccgtccatc atgtccgaag gtgcgacgat
     5641 gtccacgccg gcctccgcct gcaggatcgc ctggcgcacg aggatggagg tggtttcatc
     5701 gttgagcaca tagccgtcgt ggttgatgac gccgtcctgg ccgtggctgg tgtaggggtc
//
LOCUS       DATLGF010000279         6467 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_5916,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000279 DATLGF010000000
VERSION     DATLGF010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5916"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..520)
                     /locus_tag="VM406_08580"
     CDS             complement(119..520)
                     /locus_tag="VM406_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76058.1"
                     /translation="MSATTASNFLRKVLLADGVGAAAGAVALVALSAPLSALLGLPQL
                     LLTIAGVLLGLIAAGVLWLASRERMPRAGVWAVIAVNVVWVLDSLLLLVSGYVNPTAF
                     GQAFIVAQAAVVAVFAELEYIGLRRPQAVTA"
     gene            complement(608..2695)
                     /locus_tag="VM406_08585"
     CDS             complement(608..2695)
                     /locus_tag="VM406_08585"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVL76059.1"
                     /translation="MQELALLEPDNPSEALFQRLFRKHSSIMLLIDPSNGRIKDANRA
                     AADFYGYAEDVLRTMRVSQINVLPDEEIRQEMQRAMQEQRNYFIFRHRLSSGELRTVE
                     VHSSAIEARGTTLLFSIVFDITERKRAEEQLRLASLVYRNSSEAMSVVDANGMIMTVN
                     PAFTEITGYSAEEAIGRHMSLIDSPRATRKIYRAMRRTLDENGRWTGELWGRRRNGQE
                     FLRWLSISTIYNDDGSVQSRVGLFSDITRRREADEMIWRQANFDALTDLPNRSMFRDR
                     LEQAIKKAHRADQQVALLFLDLDSFKEINDTLGHAVGDRVLQAVAQRLQRCVRDSDTV
                     ARLGGDEFTVIIGELAEKNSIERVAQQILHVLSDPFHIGGEQLYASASIGITFYPDDG
                     TRVDELLKHADQAMYAAKAQGRNCYSYFTRAMQEAALTRKRLVTDLRRALAEGEFCLH
                     YQPIVDLATGAVHKAEALVRWNHPARGLVSPSEFIAVAEETGIIGEIGDWVFREAVRQ
                     ACVWRAGIDPDFQISINVSPAQFLHGGRDSGWIRRLEEIGLPGAGVVLEITEGLLMDG
                     RPETIATLLHLRGYGIQIALDDFGTGYSSLAYLKKFRIDFIKIDQSFVRNLAPGSDDL
                     ALCEAMIVMAHKLGIRLVAEGVETEAQRGLLAANGCDHGQGYLFARPLPAAAFEEWMR
                     RGKAPRPATAPIT"
     gene            2910..3872
                     /locus_tag="VM406_08590"
     CDS             2910..3872
                     /locus_tag="VM406_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HVL76060.1"
                     /translation="MHPPTPPCQRTRMLETMLLIRTHEETLATLHASEGVGGTCTSVG
                     QEACAVGVVQALEASDRILTNHRSAGHLLARGADPGRMMAEVLGRSTGYCKGKSGSLH
                     ISVKELGVLLTSTIVGGELSLAPGVGLSLAMQGEDAIAVCFFGDGAACEGRFHESLNL
                     AAVWKLPILYVCENNQWQAFVHRKETMLAEQIANWANGYGMPCETVDGNDVEAVLAAA
                     LRARTRMRTAGGPYFLELNTYRQRGHFEPDDQAYVDGHELQAWLARDPIALMRQRLAG
                     EGLLGEEESERMLMRVRARIDEALRFARSSPFPDPRELTTEIYA"
     gene            3892..4866
                     /locus_tag="VM406_08595"
     CDS             3892..4866
                     /locus_tag="VM406_08595"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HVL76061.1"
                     /translation="MSRMTVNEAIGRALAEEMRRDPRVLMFGEGVATKRTDLLEEFGA
                     ARVRNTPLAEGIIAGTAAGAAATGLRPVIDLLFAPFLCYAMDEIVNSAGKLRYISGGQ
                     FSFPMVVIAMTGSGWTVGAQHNHNLEAWFVHSPGLKVVMPSNAADFKGLLKAAIRDDN
                     PVLFFVDMALTPVEGEVPDDERVIPLGKAAIARAGRDLTIVSYAKTVGTCLDAAAQLA
                     ADGIEAEVIDLRSLKPLDEETILRSARKTGRVIVVHEASRMCGVGAEVAALIAEQAFP
                     SLKAPVMRLTGPDAPAPSSWALEQAFTPQADRVVRAVHELVRAHAEPA"
     gene            4911..5318
                     /locus_tag="VM406_08600"
     CDS             4911..5318
                     /locus_tag="VM406_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76062.1"
                     /translation="MQPALSTRFSDAGAIWLKLAVLYLIIGVAIGIMMGASQDFRLRP
                     VHAHLNLLGWATMALAGLTYSVYEEAGRSVLARIHFWLANISLPVMGVSLSLILFGHT
                     FVVPALIVSEVLAAAGVLVFAANLFLNLGSRRR"
     gene            5379..5861
                     /locus_tag="VM406_08605"
     CDS             5379..5861
                     /locus_tag="VM406_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76063.1"
                     /translation="MNQSAVLDADSAAFLALGCSINAGAYGADRIPVTARCCGCNVTP
                     DRTALTVFLSSRQGAAVVRAVRDNGAIAVVFSQPSTHRTLQIKGRDALVVLPEPEDLD
                     RVARYRHAFGEELRACGYDPLLAHTILSHPPADIVGLRFSPSEVYLQTPGPKAGERLY
                     "
     gene            5869..>6467
                     /locus_tag="VM406_08610"
     CDS             5869..>6467
                     /locus_tag="VM406_08610"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HVL76064.1"
                     /translation="MTVTLNAIRECFEGALPAIIATAADNGMPNVAYSSQVQFIDDAH
                     VALSYQFFNKTHKNIMVNPRAMLTVIHPGTVQQYRLTLHYLHTETSGPLFESMKAKLA
                     GIASQTGMSGVFRLLGADVFRVLGIEAVEGATLPAKPPRCNLLAALRAWSMHVAHAAD
                     LETLLADTLSHLDRQFGIRHAMLLMLDANQQRLFTVASIG"
BASE COUNT         1095 a         2166 c         2102 g         1104 t
ORIGIN      
        1 atggatcttc tcgacggagg tgtgtgatgc gcaaaatgat ctttctcgcg gtggccggat
       61 tcctgtggcg ccaggtgcgc acacggctcg gccggcgggc tttccgcggc cggccgggct
      121 aggccgtgac ggcctgcggg cggcgcaggc cgatgtactc aagctcggcg aagaccgcca
      181 ccaccgcggc ctgggcaacg atgaatgcct ggccgaaggc ggtcggattc acatagcccg
      241 acaccagcag caacaggctg tcgagcaccc agacgacatt gacggcgatc accgcccaga
      301 ccccggcgcg cggcatccgt tcgcggctgg cgagccacaa gacgccggcc gcgatcaggc
      361 cgagcagtac gccggcaatg gtgagcagca gctgcggaag cccgagcaac gccgacagcg
      421 gggcgctcag cgcgacgagg gcgacggcgc ccgctgcggc gccgacaccg tcggcgagca
      481 ggaccttgcg caggaaattg gaagcagtgg tggcggacat ggcagactcc tgttgaggtt
      541 tgaaatcggc ccgtcgggcc ggctcggagc cagtgtcgtt cgtccggtgc ggcgggtcga
      601 ttacccctca ggtaatcggc gcggttgccg gtcgcggcgc cttcccgcgc cgcatccatt
      661 cctcgaaggc cgccgccggc aacgggcgcg caaacaggta tccctggccg tggtcgcagc
      721 cgttggctgc cagcagcccg cgctgcgcct cggtttccac gccttccgca acgagccgta
      781 tgccaagctt gtgggccatc acgatcatcg cctcgcacag cgcgagatcg tccgaaccgg
      841 gcgcgaggtt gcgcacgaag gattggtcga tcttgatgaa gtcgatgcgg aatttcttca
      901 gataggccag cgatgagtag ccggttccga aatcatcgag cgcaatctgg atgccgtacc
      961 cgcgcaggtg cagcagggtc gcgatcgttt ccgggcggcc gtccatcagc aggccctcgg
     1021 tgatctccag cacgacgccg gcgccgggca ggccgatctc ctcgagccgt cggatccagc
     1081 cgctgtcgcg cccaccgtgg agaaactgtg cgggcgaaac attgatgctg atctgaaaat
     1141 cgggatcgat gccggcgcgc cagacgcagg cttgccgcac cgcttcacga aacacccagt
     1201 cgccgatttc gccgatgatg ccggtctctt ccgcgacggc gatgaattcc gacggactca
     1261 ccaacccgcg tgccggatgg ttccagcgca ccagcgcctc ggccttgtgg accgcgccgg
     1321 tggcgaggtc gacgataggc tggtaatgca ggcagaactc gccttcggcc agcgcacggc
     1381 gcagatcggt gaccagacgc ttgcgcgtca gcgcggcttc ctgcatcgca cgggtgaagt
     1441 agctgtagca gttgcgcccc tgcgccttgg ccgcatacat cgcctggtcc gcgtgcttga
     1501 gcagttcgtc gacgcgggtg ccgtcgtccg gatagaacgt gattccgatg ctcgccgatg
     1561 cgtacaactg ctcgccgccg atatggaacg ggtccgacag cacgtgcagg atctgctgcg
     1621 caacgcgctc gatgctgttc ttctcggcga gctcgccgat gatgacggtg aattcgtcgc
     1681 cgcccagacg ggcgacggta tcggagtcgc gcacgcagcg ctgcaggcgc tgggcgaccg
     1741 cctgcagcac gcgatcgccg accgcgtggc caagggtgtc gttgatctcc ttgaacgagt
     1801 ccaggtcgag gaacagcagc gcgacctgct ggtcggcgcg atgcgccttc ttgattgcct
     1861 gctcgaggcg atcgcggaac atgcttcggt tcggcaggtc ggtcagcgca tcgaaattgg
     1921 cctgacgcca gatcatctcg tcggcctcgc gccggcgcgt gatgtcggag aacagcccga
     1981 cgcggctctg cacgctgccg tcgtcgttgt agatcgtgct gatcgacaac cagcgcagga
     2041 attcctggcc gttcctgcgc ctgccccaca gctcgccggt ccagcgtccg ttttcgtcca
     2101 gcgtgcgccg catcgcccgg tagatcttgc gggtggcgcg cggcgaatcg atcaggctca
     2161 tgtggcggcc gatcgcttct tcggcgctgt agccggtgat ctcggtgaac gcgggattga
     2221 cggtcatgat catgccgttg gcgtcgacca cggacatcgc ctcgctgctg ttgcggtaca
     2281 ccagtgaggc caggcgcagc tgctcctcgg cgcgcttgcg ctcggtgatg tcaaacacga
     2341 tcgaaaacaa cagggtcgtc cctcgggcct cgatggcgga ggaatgaacc tcgacggtac
     2401 gcagctcccc gctggagagc cggtggcgga agatgaagta gttgcgctgc tcctgcatcg
     2461 cacgctgcat ctcctgccgg atctcctcat cgggcagcac gttgatctgg ctcactcgca
     2521 tcgtgcgcag cacatcctcg gcgtacccgt agaagtcggc ggccgcgcga ttggcatcct
     2581 tgattcgccc gttggacggg tcgatcagca gcatgatcga actgtgcttg cggaacaggc
     2641 gctgaaacag cgcttcactc ggattgtccg gctccaggag ggcgagctct tgcatggcgt
     2701 caggtgcggg aagaacgagg caccttaaca tggagcaaca aaaatttcct catcatcgca
     2761 tcaaggtttg cgcgacgctc gccggttact aaaactttat tcaattgatc ttaaaaaaac
     2821 gctaagcacc cgccggcctg ctccgcgctc cccgctcccg gcttagactt cgtcgcctcg
     2881 cgcaactctc tcgacgacga aggaagatca tgcatccgcc gactcccccc tgccagcgca
     2941 cgcgcatgct ggaaaccatg ctgctgatcc gcacccatga agaaacgctc gccacattgc
     3001 acgccagcga aggcgtcggc ggcacctgca cctcggtagg ccaggaagcc tgcgcggtcg
     3061 gcgtggtcca ggcgctcgaa gcgtccgatc gcatcctcac caaccatcgc agcgccggac
     3121 acctgctcgc gcgcggcgcc gaccccggtc gcatgatggc tgaagtgctg ggacgcagca
     3181 ccggctactg caagggcaag agcggctcgc tgcacatctc ggtcaaggaa ctcggcgtgc
     3241 tgctgacctc gaccatcgtg ggcggcgaac tgtcgctggc gcccggcgtg gggctgtcgt
     3301 tggccatgca aggcgaggat gcgatcgccg tgtgcttctt cggcgacggc gccgcctgcg
     3361 aggggcgttt tcacgagtcg ctcaacctcg ccgcggtgtg gaaactgccg attctctatg
     3421 tttgcgaaaa caaccagtgg caggccttcg tgcatcgcaa ggaaacgatg ctggccgagc
     3481 agatcgcaaa ctgggcgaac ggctacggca tgccgtgcga gacggtcgac ggcaacgacg
     3541 tggaggccgt cctggccgcc gcactgcgtg cgcgcacgcg catgcgcacc gccggcggcc
     3601 cgtatttcct ggaactgaac acctatcgcc aacgcggcca cttcgagccg gatgaccagg
     3661 cctacgtcga cgggcacgag ctgcaggcct ggctcgcacg ggacccgatt gcgctgatgc
     3721 ggcagcgcct ggccggcgaa ggcctgcttg gcgaggagga gagcgaacgg atgctgatgc
     3781 gcgtgcgtgc gcgcatcgac gaggcgctgc gctttgcccg cagttcgccg tttcccgatc
     3841 cgcgcgagct gactaccgag atctacgcat gatcctttcc cggagaccaa catgtcacgc
     3901 atgactgtca atgaagcgat cggccgcgcg ctggccgaag agatgcggcg cgacccgcgc
     3961 gtcctgatgt tcggcgaggg tgtcgcgacc aagcgcaccg atctgctgga agagttcggc
     4021 gcggcgcggg tgcgcaacac gccgctggcg gagggcatca tcgccggcac cgcggccggc
     4081 gcagccgcga caggcctgcg cccggtgatc gacctgctgt tcgcgccctt tctctgctat
     4141 gcgatggacg agatcgtcaa cagcgccggc aagctgcgct acatctcggg cggccagttc
     4201 tcgtttccga tggtggtgat cgcgatgacc ggcagcggct ggaccgtcgg cgcgcagcac
     4261 aaccacaatc tggaagcctg gttcgtgcac agccccgggc tgaaagtggt gatgccttcg
     4321 aacgcggccg acttcaaggg actgctcaag gccgccatcc gcgatgacaa tccggtgctg
     4381 ttcttcgtcg acatggcgct gacgccggtc gagggcgagg tcccggacga cgaaagggtg
     4441 atcccgcttg gcaaggccgc catcgcgcgc gccggccgcg acctgaccat cgtctcctac
     4501 gcgaagacgg tcggcacctg cctggatgcg gccgcgcagc tggcggccga cggcatcgag
     4561 gccgaagtca tcgatctgcg ctcgctgaaa ccgctggacg aggagaccat cctgcgctcc
     4621 gcgcgcaaga ctggccgggt aatcgttgtt cacgaagcca gccgcatgtg cggagtgggc
     4681 gcggaagtcg cggccctgat cgccgagcag gcgtttccca gcctcaaggc cccggtcatg
     4741 cggctgaccg gcccggatgc gcccgcgccc tcgagctggg cgctggagca ggccttcaca
     4801 ccgcaagccg accgcgtcgt gcgcgccgtg catgagctcg tgcgcgcgca tgcggaacct
     4861 gcgtagcctc aaccgcctga accctcacac ctcaagaaag gaaccatccc atgcaacccg
     4921 cactctccac ccgcttctcg gacgccggcg cgatctggct caagctcgcg gtcctgtacc
     4981 tgatcatcgg cgtggccatc ggcatcatga tgggcgccag ccaggacttc cggctgcgtc
     5041 cggtgcacgc ccacctgaac ctgctcggct gggcgacgat ggcgctcgcc ggcctgacct
     5101 acagcgtgta cgaagaggcc ggacgaagcg tgctggcgcg catccatttc tggctggcca
     5161 acatatcgct gccggtgatg ggcgtatcgc tcagcctgat tctgttcggc cataccttcg
     5221 tcgtgccggc cctcatcgtt tccgaggtgc tggcggcggc cggcgtcctg gtgttcgccg
     5281 ccaacctgtt cctcaatctg ggaagccgca ggcgctgagc ttcaccgggg gtggcggggc
     5341 gggtatgatt tgcatgcccg cagcttccgt gccccgtcat gaaccagtcc gctgtactcg
     5401 acgccgactc cgcagccttc ctcgcgctgg gatgctcgat caacgccggc gcatacgggg
     5461 ccgaccgcat tcccgtcacc gcgcgctgct gcggctgcaa cgtcacgccg gaccggaccg
     5521 cgctgacggt attcctgtcc tcccggcagg gcgccgcggt agtgcgcgcg gtacgcgaca
     5581 acggagcaat cgccgtggta ttcagccagc caagtacgca caggaccttg cagatcaagg
     5641 gtcgcgacgc gcttgtcgtg cttcccgaac cggaggacct cgatcgggtc gcgcgctacc
     5701 ggcacgcgtt cggcgaggag ctgcgggcgt gcggatacga tccgctgctg gcccatacca
     5761 tcctctccca tccgccggca gatatcgtcg gcctgcgctt ctcgccgagc gaggtgtatc
     5821 tgcagactcc cggcccgaag gccggcgaac gactctactg agcgcccgat gacagtcacc
     5881 ctcaacgcga tccgcgagtg cttcgaaggc gcgctgcctg cgatcatcgc cacggccgcg
     5941 gacaacggca tgccgaacgt tgcctattcg tcgcaggtgc agttcatcga cgacgcgcat
     6001 gtggcgctgt cctaccagtt cttcaacaag acgcacaaga acatcatggt caacccgcgc
     6061 gcgatgctga ccgtgatcca tcccggcacc gtgcagcagt accggctgac gctgcactat
     6121 ctgcatacgg agacgtcggg accgctgttc gaaagcatga aggccaagct ggccggcatc
     6181 gcgtcgcaaa cgggcatgag cggcgtgttc cgcctgctcg gcgccgacgt gttccgcgtg
     6241 ctcggcattg aagccgtgga aggcgcgacg ctcccggcca agccgccgcg ctgcaacctg
     6301 ctcgccgcgc tgcgcgcctg gagcatgcat gttgcgcatg ccgccgatct cgagacgctg
     6361 ctggccgaca cgctctctca tctggaccgc cagttcggca tccgtcatgc gatgctgctg
     6421 atgctcgacg cgaaccagca acggctgttc accgtggcca gcatcgg
//
LOCUS       DATLGF010000280         8388 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_28045,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000280 DATLGF010000000
VERSION     DATLGF010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 8388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8388
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28045"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VM406_08615"
     CDS             complement(<1..196)
                     /locus_tag="VM406_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVL76065.1"
                     /translation="MGVKALFSLEGRVALVTGGSRGLGLQMAEALGEMGCRLALSARK
                     ADELEEARTRLEGFGYEVQTV"
     gene            complement(471..1043)
                     /locus_tag="VM406_08620"
     CDS             complement(471..1043)
                     /locus_tag="VM406_08620"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HVL76066.1"
                     /translation="MTGAALPREFYARPTVDVARDLLGMQLIHRVDGTVRRGRIVEVE
                     AYVGAHDLASHSSRGVTPRTSVMYGPPGHAYVYLIYGMHHCVNVVTEPAGHGAAVLLR
                     ALEPLDSLAGNTRGPGLLCRTMGITLARNGIDLTGDVLFITLPPEAERFEIVQSARVG
                     VAYAGDWADAPLRFSIAGNPYVSRASGSRR"
     gene            complement(1045..4173)
                     /locus_tag="VM406_08625"
     CDS             complement(1045..4173)
                     /locus_tag="VM406_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVL76067.1"
                     /translation="MLSIPLSPPQPARPHHLRRALLLFAIMLAAVLVLGYAVFRALVE
                     REEQQTLNTLNYVANIQGVAVASWVDERTRDAAAFSDGRFLGEAMHEWISRGAPPDQT
                     RERILGQLHAIRNAFGYEEVAVVGPYGEPYLTTSAAGRNSPMLSSAIAYQTVQAALGR
                     NSPQVSTIHAGSGPEPDHRVIDIATPLLDMYERTYKAQTVLLMRADAVNQLEHYVKAM
                     PLVNTATEVVLVEIIGNEVMSTSSSHNAVHFRAFGQVPLTPQELITAARQGQEVTLSS
                     PAGTSIGVVRAVRGTPWFLLAMIDAGAVRANFERLAWLVALTAGGALAVSAFALSLWT
                     RERESRFRLQSFQAATEKALLQRRYDYLSRYANDMIILTDSDGVMQEVNDKTLQLLGR
                     ERNSVLGKSIETLFLPECRPVVRAALDSLRERGETRFEVSQRGYLGTLLTFDASARAL
                     ERDRKVIAQFIFRDISERRASEAALRDSQQRLNSILTSISDVVWSFSADFSRLNYINQ
                     SVERVFGRPLEEFIDRPILWLESMYPEDRRRFEGAIAQLSAEDPVADIEVRIVARDGQ
                     VRWLHCRGHLVTDEDGKPQRIDGVATDITQKKEAERQVEKLAYYDSVTQLPNRALLHD
                     RLGQALPMAVRSERKVALLFMDLDNFKNINDSLGHEIGDQLLRAIARRILRCVREEDT
                     VARIGGDEFLIVLPDLERASDAVRVAEKILSTAARPFDLAAHRIYTTISIGISVYPDD
                     AGDPQELIRHADSALYQAKGMGRNNYQFFTPELNYQITRTSEIERRLRHAIDNGTLAL
                     WYQPQVDARNGKLIGAEALTRWHDGEREYLSPAEFIPVAEERGLISKIGEWALREACM
                     QCRRWQEQGLEPVRVAVNVSPLQFQQKGFVDLVTGILAETGVDGRYLELEITEGALMR
                     GGAEAAPLTSKLREHGVGISIDDFGTGYSSLNYLRQIPIDKIKIDRSFISDLGRDGAI
                     TRAIVELAHSLQLRVIAEGVESQNQIERLRAFGCDEIQGYYFSRAVSPEAFARLLADR
                     YAFAHGAASTLH"
     gene            complement(4255..5430)
                     /locus_tag="VM406_08630"
     CDS             complement(4255..5430)
                     /locus_tag="VM406_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HVL76068.1"
                     /translation="MSKAQFHWDDPLLLDQQLTDDERMVRDAARAYCQDKLKPRVLEA
                     FRHEKTDPAIFREMGSLGLLGPTIPEQYGGPGLNYVSYGLIAREVERVDSGYRSMMSV
                     QSSLVMVPIFEFGNEETRQKYLPKLATGEWIGCFGLTEPNHGSDPGSMVTRAKKVDGG
                     YSLTGSKMWISNSPIADVFVVWAKDDEGAIRGFVLEKGWKGLSAPAIHGKVGLRASIT
                     GEIVMDGVFCPEENAFPDVRGLKGPFTCLNSARYGIAWGALGAAEDCWFTARQYVLDR
                     KQFGKPLAANQLIQKKLADMQTEITLGLQGCLRLGRMKDEGTAAVEITSIMKRNSCGK
                     ALDIARLARDMLGGNGISDEFGVARHMVNLEVVNTYEGTHDIHALILGRAQTGIAAF"
     gene            complement(5487..6614)
                     /locus_tag="VM406_08635"
     CDS             complement(5487..6614)
                     /locus_tag="VM406_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVL76069.1"
                     /translation="MDFSFTEEQQMLLDATRRFIAKDYTFETRRRILAESAEGFSRQV
                     WATLADIGLLSLNLPEEDGGMGGGTIENMLAMTAVGEGLMLEPYLASAVLGTRTIAAL
                     GTPAQREQWLPALAGGEQIAALAHDEPGTRFARMNVTTRADSDNDGYVLHGHKSVIAH
                     GGHADLLLVSARNGKDDGIAVFCVPRTTPGVRVLAYPTVDGVGAADIWFDGVRLPATA
                     RLGESPDASAALEDVLDFGLAAVCAEAVGALDRLMAATVEYTRNRKQFGQPIGKFQAL
                     QHRMADMLLHIEQARSISYLAAVRCTDADRAARAKALSAAKVVIGQACRFVGQEAVQL
                     HGGMGMSDELDVSHYFKRLMAIELQFGSTDHHLEQYAQSLG"
     gene            complement(6625..7821)
                     /locus_tag="VM406_08640"
     CDS             complement(6625..7821)
                     /locus_tag="VM406_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVL76070.1"
                     /translation="MDLNFTPEEQAFRQDVRDFVAKNLPQDISHKVLNGLVLESGDYI
                     RWQKTLHANGWGGFSWPKEFGGTGWSATQQYIFDEECAAAGAPRSIPFGLKMVAPVIM
                     RYGSPAQQQRFLPKIMSADEWWCQGYSEPGAGSDLASLKTRAVLDNDHYVVNGQKTWT
                     TQGQHADWIFCLVRTDPDAKPQRGISFLLIDMKSPGVTVRPIITLDGAHEVNEVWLEN
                     VRVPVENRVGEENAGWTYAKFLLGHERTNIAGIGIAKRELARLKRIAASETKNGRPLL
                     RDPYFAAKIAQVEIDLTALEITNLRVLSAEAQQRAPGPEASILKIKGTEIQQTITELL
                     VQAVGTYGLQLRREAMAAGYQGELAGPAYATPLGPHYLNMRKLSIFGGSNEIQKNIIA
                     QMILGL"
     gene            complement(8035..>8388)
                     /locus_tag="VM406_08645"
     CDS             complement(8035..>8388)
                     /locus_tag="VM406_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140074.1"
                     /note="catalyzes the thiolytic cleavage of
                     beta-ketoadipyl-CoA to succinate and acetyl-CoA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HVL76071.1"
                     /translation="AAVAGVEPRVMGLGPVYAIRKALARAKLSLADMDIIEINEAFAS
                     QVLGCLKLLEVDFADSRVNPNGGAIALGHPLGASGARLALTASRELARRNGRYAVVSL
                     CIGVGQGLAVVLERA"
BASE COUNT         1381 a         2791 c         2696 g         1520 t
ORIGIN      
        1 cgacggtctg cacttcatag ccgaagcctt cgaggcgggt gcgcgcctcc tccagttcat
       61 ccgccttgcg cgccgacagc gcgagccggc atcccatttc gccgagcgcc tcggccatct
      121 gcaggccgag accccgcgag ccgccggtca cgagcgcgac cctgccttcc aggctgaaca
      181 acgccttcac gcccatcctg tctccttggc cgctgccctg cggctcgaat tttgtccttg
      241 gctgcgattc tgcagagtga aataaagttc tgcattatta tcgcgatgat agtaccaagg
      301 ccggtacggc gctgtaaagg cgcgccggct gccgggaaag catctgatga aggagggtca
      361 gtcgtattga tgttccacaa cctggagccg gcctgcccgc gaatgggatg tgcagtctgc
      421 agtgcagaat taaaacgacg acagcgactc gcgtgactac gactcctgtg tcagcggcgg
      481 gagccactgg cgcgcgacac ataaggattg ccggcgatgg aaaagcgcag cggcgcatcg
      541 gcccagtcgc ccgcgtatgc cacgcccacg cgcgcactct gcacgatctc gaaacgttcc
      601 gcctctggcg gcagcgtgat gaacagcacg tcacccgtga gatcaatgcc gttgcgcgcc
      661 agcgtgattc ccatcgtgcg gcacagcaag cctgggccgc gggtattgcc ggccaggctg
      721 tccagcggtt cgagcgcgcg cagcagcacc gccgcgccgt ggcccgccgg ctcggtgacc
      781 acgttcacgc aatggtgcat cccgtagatc aggtagacat aggcatggcc gggcggaccg
      841 tacatgaccg aggtgcgcgg cgtgacgcct cgcgacgagt gcgaggcgag atcatgcgca
      901 ccgacatagg cctcgacttc aacgatgcgc ccgcgccgca ccgtgccgtc gacccggtga
      961 atcaactgca tgccgagcag atcccgcgca acgtcgacgg tgggacgggc atagaactcg
     1021 cggggcaggg cggcacctgt catatcaatg cagtgtggag gccgcgccgt gcgcgaacgc
     1081 gtacctgtcg gcgagcagcc gggcgaacgc ttccggcgac acggcccggc tgaagtaata
     1141 cccctgaatc tcgtcgcacc cgaatgcgcg caggcgttcg atctggttct gcgattccac
     1201 cccctcggcg atgacgcgca gctgcaggct gtgggccagc tcgacaatgg cgcgggtgat
     1261 tgccccgtcg cggcccaggt ccgagatgaa cgagcgatcg atcttgatct tgtcgatcgg
     1321 gatctgccgc aaatagttca ggctggagta gccggttccg aaatcgtcga tgctgatgcc
     1381 gaccccgtgc tcgcgcagct tcgaggtgag cggcgctgcc tccgcgccgc cgcgcatcaa
     1441 cgcgccttcg gtgatttcca gctccaggta gcgcccgtcc acgccggttt ctgcaaggat
     1501 gccggtgacg agatcgacga aacccttctg ctggaactgg agtggggaga cgttgacggc
     1561 cacgcgcacc ggttccaggc cctgttcctg ccagcgccgg cactgcatgc aggcctcgcg
     1621 cagcgcccat tctccgatct tgctgatcaa cccgcgctcc tccgccaccg ggatgaactc
     1681 ggccggcgac aggtattcgc gctcgccgtc atgccagcgc gtgagcgcct ccgccccgat
     1741 cagcttgccg ttgcgcgcat cgacctgcgg ctggtaccaa agcgccagcg tgccgttgtc
     1801 gatcgcatgg cgcaggcgcc gctcgatttc cgaggtgcgc gtgatctggt agttcagttc
     1861 cggcgtaaag aactgatagt tgttgcggcc catgcccttg gcctgataga gggcggaatc
     1921 ggcgtgccgg atcagttcct gcgggtcgcc cgcgtcatcc gggtacacgc tgatgccgat
     1981 cgagattgtc gtgtagatgc ggtgggccgc cagatcgaag ggccgggcgg ccgtggaaag
     2041 gatcttctcc gccaccctga ccgcgtcgct tgcgcgttcg aggtcaggga ggacgatcag
     2101 gaattcgtct ccgccgatgc gcgccaccgt gtcctcctcg cgcacgcagc ggagaatgcg
     2161 acgcgcaatg gcgcgcagca gctggtcgcc gatctcgtgg ccgagggaat cattgatgtt
     2221 cttgaaattg tccaggtcca tgaacagcag ggcgaccttg cgctcggagc gcacggccat
     2281 cggcagcgcc tgtcccaggc ggtcgtgcag gagcgcgcgg ttgggcaact gggtcacgct
     2341 gtcgtagtag gccagcttct cgacctgccg ctcggcttct ttcttctgcg tgatgtcggt
     2401 cgccacgccg tcgatgcgct gcggcttgcc gtcttcatcc gtgaccagat gtccgcggca
     2461 atgcagccag cgcacctggc cgtcgcgcgc gacgatgcgc acttcgatgt cggcgaccgg
     2521 atcttcggcg gacaattgcg caatcgcgcc ttcgaagcgg cggcggtctt ccggatacat
     2581 ggactccagc cacaggatgg gccggtcaat gaactcctcg agcgggcgcc cgaacacccg
     2641 ttcgacggac tggttgatgt agttcaggcg cgagaagtcg gcgctgaagg accacacgac
     2701 gtcgctgatc gacgtcagga tgctgttgag ccgctgctgg ctgtcgcgca acgctgcttc
     2761 cgaggcgcgg cgctccgaga tgtcacggaa gatgaactgg gcaatgacct tgcggtcgcg
     2821 ttcgagcgcg cgcgcactcg catcgaaggt cagcagcgtg ccgagatagc cgcgctgcga
     2881 cacttcgaaa cgcgtctcgc cgcgttcgcg cagcgagtcc agtgccgcgc gcacgaccgg
     2941 ccggcattcc ggcaggaaca gtgtctcgat cgacttgccg agcacgctgt tgcgctcgcg
     3001 ccccagcagc tgcaaagtct tgtcgttgac ttcctgcatg acgccgtcgc tgtctgtcag
     3061 gatgatcatg tcgttcgcat agcgcgacag gtagtcgtag cgtcgctgga gcagcgcctt
     3121 ctcggttgcc gcctgaaacg actgcaggcg gaagcggctt tcgcgctcgc gcgtccagag
     3181 gctcagcgcg aacgcgctca ccgccagggc cccgccggcg gtcagcgcca ccagccaggc
     3241 cagccgttcg aagttcgcgc gcacggcacc ggcatcgatc atcgcaagca ggaaccatgg
     3301 cgtgccgcgc acggcgcgca cgaccccgat gctcgtgccc gccggtgacg agagggtcac
     3361 ttcctgccct tgccttgccg cggtgatcag ctcttgaggc gtcagcggca cttgcccgaa
     3421 agcgcggaaa tgcacagcgt tgtggctgga cgacgtcgac atcacttcgt tgccgatgat
     3481 ctccacgagc acgacttcgg tcgcggtatt gaccaggggc atcgccttca cgtaatgctc
     3541 gagctggttc accgcatcgg cgcgcatcag caagacggtc tgggccttgt aggtgcgctc
     3601 gtacatgtcc agcaacggcg tggcgatgtc gattacgcgg tgatcgggtt cggggccgga
     3661 gccagcgtga atcgtcgaca cctgcgggct gttccggcct agcgcggcct ggacggtctg
     3721 ataggcaatt gccgaagaca gcatcggact gttgcgtccg gctgcgctgg tggtcaggta
     3781 gggctcgcca tacggcccca cgacggcgac ctcctcgtag ccgaaggcgt ttctgatggc
     3841 gtgcagctgc ccgagaatgc gctcgcgggt ctggtcgggc ggtgcgccgc gcgatatcca
     3901 ttcatgcatc gcctcgccca ggaagcgccc gtccgaaaac gcggcggcgt cgcgggtacg
     3961 ttcgtcgacc catgaagcca cagccacccc ctggatgttg gcgacgtagt tcagcgtgtt
     4021 gagggtctgc tgctcttcgc gttcgaccaa tgcccggaac actgcatagc cgagcacgag
     4081 cacggccgcc agcatgattg cgaacagcag caatgcccgg cgcagatggt ggggccgggc
     4141 aggttgcggc ggcgagagcg gaatggaaag catcggcagt cattacttgc gcccagggca
     4201 acaggctcat gcatatgacc gccgacaacg attgccgttc tgccaaaccg gaaatcagaa
     4261 tgccgcgatg cccgtctgcg cgcggccgag gatcagcgca tgaatgtcat gcgtgccttc
     4321 gtaggtgttg accacctcga gattcaccat gtgccgcgcc acgccgaatt cgtcggagat
     4381 gccgttaccg cccagcatgt cgcgggcaag acgggcgatg tcgagcgcct tgccgcatga
     4441 attgcgcttc atgattgaag tgatctcgac cgccgcggtg ccttcgtcct tcatcctgcc
     4501 caggcgcagg cagccctgca ggccgagcgt gatctcggtc tgcatgtcgg ccagcttctt
     4561 ctggatcagc tggttcgccg ccagcggctt gccgaactgc ttgcggtcga gcacgtactg
     4621 gcgcgcggtg aaccagcagt cctcggcggc gcccagcgcg ccccaggcga tgccgtagcg
     4681 ggcggagttc aggcaggtga acggcccttt cagcccgcgc acgtccggga acgcgttttc
     4741 ttccggacag aacacgccgt ccatcacgat ctcgccggtg atcgacgcgc gcaggccgac
     4801 cttgccgtgg atcgccggcg cggacaggcc tttccagccc ttctccagca cgaagccgcg
     4861 gatcgcccct tcgtcgtcct tggcccagac gacgaacacg tcggcgatcg gggagttgga
     4921 gatccacatc ttgctgccgg tgaggctgta gccgccgtca accttcttcg cgcgcgtgac
     4981 catcgagccg ggatcggagc catggttcgg ctcggtcagc ccgaagcagc cgatccattc
     5041 gccggtggcc agcttgggca ggtatttctg cctggtttcc tcattgccga actcgaagat
     5101 cggcaccatg accagcgagg actgcacgct catcatcgag cggtagccgg aatccacgcg
     5161 ctcgacctcg cgcgcgatca gtccgtagga cacgtagttc agccccggcc cgccgtactg
     5221 ttccggaatg gtcgggccga gcaggcccag cgatcccatc tcgcggaaga tcgccggatc
     5281 ggtcttttcg tggcggaagg cttccagcac acgcggcttg agcttgtcct ggcaataagc
     5341 gcgggctgcg tcgcggacca tgcgttcgtc atcagtgagc tgctggtcga gcagcagcgg
     5401 atcgtcccaa tgaaactggg ctttgctcat cgtgttctcc tggagtgggg ttgcctggac
     5461 ggcagaatgc atatggtatc ccgccttcag ccgagggact gtgcgtactg ctccaggtga
     5521 tggtcggtgc tgccgaactg cagctcgata gccatcagcc gcttgaagta gtggctcacg
     5581 tccagctcgt cgctcatgcc catgccgccg tgcagctgca ccgcctcctg gccgacgaag
     5641 cggcaggcct ggccgatcac gaccttggcc gccgacaagg ctttggcacg cgcggcgcgg
     5701 tccgcatcgg tgcagcgtac ggcggccaga tagctgatcg agcgcgcctg ttcgatatgc
     5761 agcagcatgt ccgccatccg atgctgcagc gcctggaact tgccgatggg ctggccgaac
     5821 tgcttgcggt tgcgcgtgta ttcgacggtt gccgccatca gtcggtcgag cgcgccgacc
     5881 gcttccgcac acaccgcagc caggccgaag tcgagcacgt cctccagcgc tgcactggca
     5941 tccggcgact cgccgagccg cgccgttgcc ggcaggcgga ccccgtcgaa ccagatgtcg
     6001 gccgcaccca cgccatccac tgtcgggtag gcgaggacgc gcacgcccgg cgtggtacgc
     6061 ggcacgcaga acaccgctat gccgtcatcc ttgccgttgc gcgcgctcac gagcagcaga
     6121 tcggcatggc cgccgtgcgc gatcacgctc ttgtggccgt gcagcacata cccgtcgtta
     6181 tcgctgtcgg cgcgcgtggt gacgttcatg cgggcaaacc gcgttccggg ctcgtcgtgc
     6241 gcgagcgcgg cgatctgctc gccgcccgcc agcgccggca gccattgctc gcgctgcgcg
     6301 ggggttccga gtgcggcgat ggtgcgggtg ccgagcaccg cgctggcgag gtagggctca
     6361 agcatcaggc cttcgccgac ggcggtcatc gccagcatgt tctctatcgt gccgccgccc
     6421 atgccgccgt cctcttcggg caggttcagc gacagcaggc cgatgtcggc cagcgtcgcc
     6481 catacctgcc gcgaaaagcc ctccgcgctt tcggccagga tgcggcggcg cgtttcgaac
     6541 gtgtagtcct tcgcgatgaa acggcgggtc gcatcgagca gcatctgctg ctcttcggta
     6601 aaggaaaaat ccatcgcgag gctcctacag tcccaggatc atctgggcga tgatgttctt
     6661 ctgaatttcg ttcgagccgc cgaagatcga cagcttgcgc atgttcaggt agtgcgggcc
     6721 cagcggcgtc gcatacgcgg gcccggccag ctcgccctgg tagccggccg ccatcgcttc
     6781 gcgccgcagc tgcagcccgt aggtgccgac cgcctgcacc agcagctcgg tgatggtctg
     6841 ctggatttcg gttcccttga tcttcaggat cgaggcttcc ggcccgggcg cacgctgctg
     6901 cgcctcggcc gacagcacgc gcaggttggt gatctccagc gcggtcaggt cgatctcgac
     6961 ctgcgcgatc ttcgcggcga agtaggggtc gcgcagcagc ggcctgccgt tcttggtttc
     7021 ggatgcggcg atgcgcttca gacgcgcgag ttcgcgcttg gcgatgccga tgccggcgat
     7081 attcgtgcgc tcatggccga gcaggaactt cgcataggtc cagccggcgt tttcctcgcc
     7141 gacccggttt tccaccggca cgcgcacatt ctccagccac acttcattga cttcgtgcgc
     7201 gccgtcaagc gtgatgatcg gccgcaccgt cacgccggga ctcttcatgt cgatcagcag
     7261 gaaggagatg ccgcgctgcg gcttggcgtc cggatcggtg cgcacgaggc agaagatcca
     7321 gtcggcatgc tggccctgcg tggtccaggt cttctggccg ttgacgacgt agtggtcatt
     7381 gtccagcacc gcgcgcgtct tcagcgaagc gagatccgag cccgcgcccg gctccgaata
     7441 gccctggcac caccactcgt cggccgacat gatcttcggc aggaagcgct gctgctgcgc
     7501 gggcgaaccg tagcgcatga tcaccggcgc gaccatcttg agtccgaacg gaatcgaacg
     7561 cggcgcgccg gcagccgcgc attcctcgtc gaagatgtac tgctgcgtcg cgctccagcc
     7621 cgtgccgccg aattccttcg gccacgagaa gccgccccag ccgttggcat gcaaggtctt
     7681 ctgccagcgg atgtagtcgc cgctttcgag caccagcccg ttcagcacct tgtggctgat
     7741 gtcctgcggc aggttcttcg cgacgaaatc gcgtacgtcc tgcctgaacg cctgctcttc
     7801 aggcgtgaaa ttcaaatcca tgtgttcctc atagtgtcga tttgggggat gaggtccttc
     7861 gcttgcgttc aggacgacaa cgatccacca cactgtcatc ccgagccacc aacgtgtcat
     7921 cctgagccgc aggcgaagga tctcgtccgc ccggtccgcc aacggcgctg aaacggaggg
     7981 atggggtcct ttgctcacgc tcgggacgac actgcgtggt agacacgcaa gccctcacgc
     8041 ccgctccagc accaccgcca gcccttgtcc cacgccgatg caaagactca ccaccgcata
     8101 gcgtccattg cgccgcgcca gttcccggct ggctgtcagc gcgagtcgcg cgccggaggc
     8161 gcccagcgga tggccgagcg cgatcgcgcc gccgttcggg ttcacgcggc tgtcggcaaa
     8221 gtcgacttcc agcagcttca ggcagcccag cacctggctc gcaaacgctt cgttgatctc
     8281 gatgatgtcc atgtcggcga gcgacagctt cgcccgcgcg agcgccttgc ggatcgcata
     8341 caccggcccg aggcccatca cgcgcggctc cacgccggcg accgcggc
//
LOCUS       DATLGF010000281         2016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_5929,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000281 DATLGF010000000
VERSION     DATLGF010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5929"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="VM406_08650"
     CDS             <1..1180
                     /locus_tag="VM406_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HVL76072.1"
                     /translation="IEEAPVVKLLQSVFDDAAQVRASDIHIEPQETRLQIRFRIDGVL
                     HLQTEADIKIAQALALRLKLMSGLDISEKRLPQDGRFAIKVKNQQMDVRISTMPTQYG
                     ESVVMRLLNQSGGMLRLDSIGMPAAMLTRFRAILQRPNGLVLVTGPTGSGKTTTLYGA
                     LAELNSVERKLITVEDPVEYRLPGINQVQVNEKIELTFARVLRSALRQDPDVVLVGEM
                     RDQETAQIGMRAALTGHLVLSTLHTNDAVSTPIRLLDMGVARYMVATSLQAVLAQRLV
                     RVICESCASPYTLKPAEQAWLRAELGDSVASHRYVHGRGCTHCNGTGYRGRTGVYEML
                     EMTEALADAANQHEPAHFIKVAQAQMAGNTLRRHAVSLVVAGKTTVSEAMRVSNQADD
                     "
     gene            1322..>2016
                     /locus_tag="VM406_08655"
     CDS             1322..>2016
                     /locus_tag="VM406_08655"
                     /inference="COORDINATES: protein motif:HMM:NF027197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin glycosylation ligase domain-containing
                     protein"
                     /protein_id="HVL76073.1"
                     /translation="MTSFYNEWLAAVLLLAVGICMLLPPLLSARRTGTIEVPKVSLLV
                     LPLLGVIAVQWFGAKLTYASDALLPIWILALTGAAMVVGNALGRQAGLPRLSAWICAG
                     IVVGALANFAMQIVQLCTEHGFRIGLIHFDTGGVYYGSLGQRNHLATYFGWALVAALY
                     LGAARRLAPTMLVSLVLVFLAGAALTASRTSWLQIVWIAAAAGFVVGRMSMQERPKGW
                     GLVVALPVLFAVV"
BASE COUNT          319 a          645 c          677 g          375 t
ORIGIN      
        1 catcgaggag gcgcccgtcg tcaagctgct gcagtcggtg ttcgatgatg ctgcccaggt
       61 ccgcgcgtcg gacatccaca tcgagccgca ggaaacgcgc ctgcagatcc gcttccgcat
      121 cgatggcgtg ctgcacctgc agaccgaggc cgacatcaag atcgcgcagg cgctcgcact
      181 gcgcctgaag ctcatgtccg gcctcgacat ctccgagaaa cgcctgccgc aggacggccg
      241 cttcgcgatc aaggtcaaga accagcagat ggacgtgcgt atctcgacga tgcccacgca
      301 gtacggcgaa tcggtggtga tgcgtctgct gaaccagagc ggcggcatgc tgcgcctgga
      361 ttcgatcggc atgccggcgg cgatgctgac gcgttttcgc gccatcttgc agcggccgaa
      421 cggtctggtc ctggtaacgg gacccaccgg cagcggcaag accaccaccc tgtacggtgc
      481 gcttgccgag ctgaattcgg tggagcgcaa gctgatcacc gtcgaggacc cggtcgagta
      541 ccggctgccc ggaatcaacc aggtgcaggt caacgagaag atcgaattga cgttcgctcg
      601 cgtgctgcgt tcggcgctgc gccaggatcc ggacgtcgtg ctggtcggcg aaatgcgtga
      661 ccaggagacc gcgcagatcg ggatgcgtgc cgcgctcacc ggccacctgg tcctgtcgac
      721 cttgcacacg aacgacgcgg tcagcacgcc gatccgtctg ctcgacatgg gcgtggcgcg
      781 ctacatggtg gctacgtcgc tgcaggcggt gctggcgcag cgcctggtgc gtgtgatctg
      841 tgaaagctgc gcgtcgcctt atacgctcaa gccggccgag caggcgtggc tgcgggccga
      901 actgggcgac agcgtcgctt cccatcgcta tgtgcacggc cgcggatgca cccactgcaa
      961 cggcacagga tatcgaggcc gcaccggcgt gtatgaaatg ctcgaaatga ccgaggcgct
     1021 tgcggacgcg gccaaccagc atgagccggc gcacttcatc aaggttgcgc aggcgcagat
     1081 ggcgggcaac acgctgcggc gccatgcggt gtcactcgtc gtcgccggca agaccacagt
     1141 gtccgaagcg atgcgggtca gcaaccaggc cgatgactga accccgtctg atgtccacgc
     1201 ccccagccgc gccatgactc ctgccagttc agaaggccaa agcgcgcctc cgaggcttgc
     1261 actggctggg cttgccgcat tggccagtct tcccttcgtc tggccattgc acacgcggcc
     1321 gttgacgagc ttctacaacg agtggctcgc ggccgtcctg ttgctcgcgg ttgggatctg
     1381 catgctgttg ccgccgttgc tgtcggcgcg gcgcaccggc acgatcgaag tgccgaaggt
     1441 ctcgctgctg gtgcttccat tgctcggcgt gatcgccgtc cagtggttcg gagcaaagct
     1501 cacctacgca tccgatgcgc tgctgccgat atggatactc gcgctgaccg gcgcggcgat
     1561 ggtggtcggc aatgccttgg gccgccaggc cgggctgccc cggctgtcgg cctggatctg
     1621 cgccggcatc gtagttggcg cgctggcgaa cttcgcgatg cagatcgtgc agctctgcac
     1681 cgagcacgga tttcgcattg ggctgattca tttcgatacc ggcggcgtct attacggcag
     1741 tctcgggcag cgcaatcacc ttgccaccta cttcggctgg gcgctggtgg ccgcgcttta
     1801 ccttggcgcg gcccgccgtc tggcgccgac catgctggtg tcgctggtcc tggttttcct
     1861 ggccggtgcg gcgctgacgg cttcgcgcac cagctggctg cagatcgtct ggatcgccgc
     1921 ggctgcgggt ttcgttgtcg ggcgcatgtc gatgcaagag cggccgaaag ggtgggggct
     1981 agtcgtggcg ttgccggtgc tgtttgcggt cgtcaa
//
LOCUS       DATLGF010000282         4709 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_118651, whole genome shotgun sequence.
ACCESSION   DATLGF010000282 DATLGF010000000
VERSION     DATLGF010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118651"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2519)
                     /locus_tag="VM406_08660"
     CDS             complement(<1..2519)
                     /locus_tag="VM406_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HVL76074.1"
                     /translation="MTELRETKATCCYCGVGCGVLVQTDGKTITGVRGDPDHPANFGR
                     LCTKGSTLHLTADPLLQQQARALQPEMRLARADQRTPVSWDTALDYVAARFTECVRRH
                     GPDSVAFYISGQLLTEDYYVFNKLAKGLIGTNNIDTNSRLCMSSAVAGYKQTLGADAP
                     PACYEDIDHAQLIFIAGSNTAYAHPILYRRIEEARKRNPALKMIVADPRRTDTARDAD
                     LFLPILPGTDVALFNGMLHICLWEDLIQPEYIAAHTEGFAELKQTLRDYTPSFVAQTC
                     GIAEKDLVTAARWFGQSPAALSLYCQGLNQSSSGTAKNAALINLHLATGQIGKPGAGP
                     FSLTGQPNAMGGREVGGMANLLSAHRELANPEHRAEVARLWGVDEVPSRPGRTAVEMF
                     EALKSGEIRMVWIACTNPAQSLPAQAMVREALKAAELVVVQDAYRTTATVPYADVLLP
                     ATTWAEKEGTVTNSERRITRFRPVLPKPGQARHDWEIVNDFARRYEAATGRARTLFPY
                     QDVESIWNEHRESTRGRDLDITGLSYRILEERGPQQWPFPERAQSGKARLYEDGVFPT
                     PSGRARFVNTIYKPVAEPADARYPFRLNTGRLRDQWHGMSRTGTVARLFSHAPEPAVM
                     MAPIDMERRLIADGDLVHVTSRRGSQILPARKSEEMRSGQVFVAMHWGEEYVSGRGQG
                     GEGTYGVNALTQPVFCPTSRQPELKHAAVKVLKAELPWRFLVFGWVDPGRALALQAAL
                     RPRLRCFSYASCTLFGRERQGVLLRAADDYAAPLEVLRDIEAQFGIVGPEVLRYDDTR
                     RGHSRHILMRDGMLEAVSLAGDTSAEQWLKEYLESGQPVDKL"
     gene            complement(2516..2863)
                     /gene="nirD"
                     /locus_tag="VM406_08665"
     CDS             complement(2516..2863)
                     /gene="nirD"
                     /locus_tag="VM406_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HVL76075.1"
                     /translation="MSQQWKAVCNVADIPVLGARVVHRAGKPDVAIFRNSEDKVFALL
                     DRCPHRGGPLSQGIVFGERVACPLHNWNIELSTGEAVAPDHGCANKFSVKIEDGRVYL
                     DITELEAPLLEPA"
     gene            complement(2946..>4709)
                     /gene="nirB"
                     /locus_tag="VM406_08670"
     CDS             complement(2946..>4709)
                     /gene="nirB"
                     /locus_tag="VM406_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HVL76076.1"
                     /translation="FTDGLEIPAELVVMAVGIRPNTALAESAGLHCNRGIVVNDTMQT
                     FDPRIYAVGECVNHRGTAYGLVAPLFEMARVCANHLANFGIGRYQGSVTSTKLKVTGI
                     DLFSAGEFMGGEGVEEIVLSDPIGGVYKKLVIKDDKLVGACLYGDTVDGSWYFSLLRE
                     QKNIAEIRDTLMFGEANTGRPGDVGHEGFTKAAAMPDTAEVCGCNGVCKGTIVKAIKE
                     QGLFTLEDVRKQTKASASCGSCTGLVEQIIMATAGGDYSTSPKVKPICGCTDHTHQQV
                     RDAIRANKLLTIADTMKFMEWRTPNGCSTCRPAINYYLISTWPHEAKDDPQSRFINER
                     AHANIQKDGTYSVVPRMWGGETNASELRRIADVVDKYNIPTVKVTGGQRIDLLGVKKE
                     DLPKVWKDLDMPSGHAYAKALRTVKTCVGSEWCRFGTQNSTLMGKQLERALWRMYAPH
                     KVKLAVSGCPRNCAESGIKDVGVIGVDSGWEIYVGGNGGIKTEVAHFFVKLKTHEEVL
                     EYSGAFLQLYREEGWYLERTVHYIARVGLDYVKKKVIEDAENRKALYERLMFSLDGEP
                     DPWHEREKAQVDERQFAPLAV"
BASE COUNT          762 a         1568 c         1504 g          875 t
ORIGIN      
        1 agcttgtcga ccggctggcc tgattccagg tattccttca gccactgttc agctgatgta
       61 tcgccggcaa gagagaccgc ctccagcatg ccgtcgcgca tcaggatgtg ccgggaatga
      121 ccgcgccggg tatcgtcgta ccgcaatacc tcgggcccga cgatgccgaa ctgggcttcg
      181 atgtcccgca gcacttccag cggcgccgcg taatcatccg ccgcacgcag caggacgccc
      241 tggcgctcgc ggccgaacaa ggtgcacgat gcataactga agcagcgcaa ccgcggccgc
      301 agcgccgcct gcagcgcgag cgcgcgcccg gggtcgaccc acccgaacac caggaagcgc
      361 cacggcagtt cggccttcag caccttgact gccgcgtgct tgagttccgg ctgccgcgag
      421 gtcgggcaga acaccggctg ggtcaaggcg ttgacgccgt aggtgccctc tccgccttgc
      481 ccgcggccgg acacatattc ctcaccccag tgcattgcga cgaagacctg cccggaacgc
      541 atttcctcgc tctttcgcgc gggaaggatc tgcgatccgc ggcggctcgt cacatgaacg
      601 agatcgccat cggcgatcag ccggcgttcc atgtcgatcg gcgccatcat caccgccggc
      661 tcgggcgcat gcgagaacag ccgcgcgacc gtgccggttc ggctcatgcc gtgccactgg
      721 tcgcgcaggc gaccggtatt gagccggaac gggtagcggg catctgccgg ctcggccacc
      781 ggcttgtaga tggtgttgac gaagcgcgcg cggcccgatg gcgtcgggaa cacgccgtct
      841 tcatacaggc gcgccttgcc cgactgcgcg cgttccggaa acggccattg ctgcggacca
      901 cgttcttcca ggatgcggta ggacagcccg gtgatgtcga gatcgcgccc gcgcgtggat
      961 tcgcggtgct cgttccagat cgactccacg tcctggtacg gaaacagcgt gcgcgcccgg
     1021 ccggtcgccg cttcgtagcg acgcgcgaaa tcgttcacga tttcccagtc gtggcgcgct
     1081 tgacccggct tcggcaaaac cggcctgaat cgcgtgatgc gccgctccga gttggtgacc
     1141 gtgccctcct tctcggccca tgttgtcgcc ggcagcagca cgtcggcgta cggaacggtg
     1201 gctgtcgtgc ggtaggcgtc ctgcacgacc accagttcgg ccgccttcaa ggcctcgcgc
     1261 accatcgcct gcgcgggcag cgactgtgcc ggattcgtgc aggcgatcca gaccatccgg
     1321 atctcgccgc tcttcagcgc ttcgaacatt tccactgcgg ttctgcccgg cctggacggc
     1381 acttcgtcca cgccccaaag tcgcgcgact tcagcgcgat gctcaggatt ggcgagctcg
     1441 cggtgcgccg acagcagatt cgccatgccg ccgacttcgc gcccgcccat tgcgttcggc
     1501 tggccggtca gcgagaacgg gccggcgccg ggcttgccga tctggcctgt cgccaggtgc
     1561 aggttgatca gcgcggcatt cttcgcagtg ccggacgacg actgattcaa gccctggcaa
     1621 tacagcgaga gggcggcggg agactgcccg aaccagcgcg cggcggtgac cagatccttc
     1681 tcggctatgc cgcaggtctg cgcgacgaaa gacggcgtgt agtcgcgcag cgtctgtttc
     1741 agctccgcga agccttcggt gtgggccgcg atgtactccg gctgaatcag gtcttcccac
     1801 aggcagatat gcagcatgcc gttgaacagc gccacgtcgg tgccgggaag tatcggcagg
     1861 aacaggtcgg catcgcgcgc cgtgtcggtg cggcggggat cggcgacgat catcttcagc
     1921 gccggattgc gcttcctcgc ctcctcgatg cggcgataga gaatcggatg cgcgtaggcg
     1981 gtgttcgatc cggcgatgaa gatcagttgc gcatggtcga tgtcttcata gcaggccggc
     2041 ggcgcgtcgg cgcccagcgt ctgcttgtag ccggcgaccg cgcttgacat gcacaggcgc
     2101 gaattggtgt cgatgttgtt cgtgccgatc aggcccttgg ccagcttgtt gaaaacgtaa
     2161 tagtcctcgg tgagcaactg cccggaaatg tagaaggcaa cactgtcggg gccgtgcctg
     2221 cgcacgcact cggtaaagcg cgcggcgaca tagtcgagcg ccgtatccca cgaaacgggc
     2281 gtgcgctggt cagcgcgggc cagccgcatc tcgggctgca acgcgcgcgc ctgctgctgc
     2341 aacagcggat cggccgtcag gtgcagcgtg ctgcccttcg tacagaggcg gccgaagttc
     2401 gccggatgat cgggatcgcc ccgcacgccg gtgatggtct tgccgtccgt ctgcacgagc
     2461 acgccgcagc cgactccgca ataacagcag gtggccttgg tttcgcgcag ttcagtcatg
     2521 cgggttccag cagcggggct tcgagctcgg tgatgtcaag gtagacgcgg ccgtcctcga
     2581 tcttcacgct gaacttgttg gcacagccgt gatcgggcgc gaccgcctcg ccggtcgaca
     2641 gttcgatgtt ccagttgtgc agcggacagg cgacccgctc gccgaacacg ataccctgtg
     2701 agagcggacc gccgcgatgc ggacagcggt cgagcagcgc gaagaccttg tcttcgctgt
     2761 tgcggaagat ggccacgtcc ggcttgccgg cgcggtgcac gacgcgcgcg ccgagtacgg
     2821 ggatgtctgc gacgttgcag acggctttcc attgttgcga catgttctat cctttacttg
     2881 ggcgcgcccg aacccgcccg cggtgagtgc gagcggaccg gtggttgtat ccatgggcca
     2941 tgcgcttata ccgccagcgg cgcgaactgg cgctcgtcga cctgcgcctt ctcgcgttcg
     3001 tgccacggat ccggctcgcc gtccagcgag aacatcaggc gctcgtacag tgccttgcgg
     3061 ttctcggcat cctcgatcac cttcttcttc acatagtcga ggccgacgcg ggcgatgtaa
     3121 tggacggtgc gctccaggta ccagccctct tcgcgataca actgcaggaa ggcacccgag
     3181 tactcgagca cttcctcatg cgtcttcagc ttgacgaaga aatgcgcgac ctcggtcttg
     3241 atgccgccgt tgccgcccac atagatctcc cagccggaat cgacgccgat cacgcccaca
     3301 tccttgatgc cggactccgc gcagttgcgc gggcagccgg acaccgccag cttcaccttg
     3361 tgcggcgcgt acatgcgcca cagcgcgcgt tcgagctgct tgcccatcag cgtcgagttc
     3421 tgggtgccga agcgacacca ttcggagccg acgcaggtct ttaccgtgcg cagcgccttc
     3481 gcgtacgcgt gaccggaagg catgtcgaga tccttccaca ccttgggcag gtcctccttc
     3541 ttcacgccga gcagatcgat gcgctggccg ccggttacct tgacggtcgg gatgttgtac
     3601 ttgtcgacca cgtcggcgat gcggcgcagc tccgacgcat tggtctcgcc gccccacatg
     3661 cgcggaacca ccgagtaggt gccgtccttc tggatgttcg cgtgcgcgcg ctcgttgatg
     3721 aagcgcgact gcggatcgtc cttcgcctca tgcggccagg tcgagatcag gtagtagttg
     3781 atcgcggggc ggcaggtcga gcagccgttc ggcgtgcgcc attccatgaa cttcatcgtg
     3841 tcggcgatcg tcagcagctt gttcgcacgg atcgcgtcgc gcacctgctg atgcgtgtga
     3901 tcggtgcagc cgcagatcgg cttgaccttg ggcgaggtcg agtaatcgcc gccggcagtc
     3961 gccatgatga tctgctcgac cagccctgtg cacgaaccgc aggaggccga tgccttcgtt
     4021 tgcttgcgca cgtcctccag cgtgaacagc ccctgctcct tgatcgcctt gacgatggtt
     4081 cccttgcaca cgccgttgca gccgcacacc tcggcggtat ccggcattgc cgccgccttg
     4141 gtgaagccct cgtggccgac gtcgccgggg cggccggtgt tcgcttcgcc gaacatcagc
     4201 gtgtcgcgga tctcggcaat gttcttctgt tcgcgcagca gactgaaata ccaggagccg
     4261 tcgacggtgt cgccgtacag gcaggcgccg accagcttgt catccttgat caccagcttc
     4321 ttgtagacgc ccccgatagg gtccgacagc acgatttcct cgacaccctc gccgcccatg
     4381 aactcgcccg cggaaaacag gtcgatgccg gtcaccttca gcttggtcga cgtgaccgat
     4441 ccctgatagc ggccgatccc gaagttcgcc aggtggttcg cgcacacccg cgccatctcg
     4501 aacagcggcg caaccagccc gtaggcggtg ccgcgatggt tcacgcactc gcccaccgca
     4561 tagatgcgcg gatcgaaggt ctgcatcgtg tcgttgacca cgatgccgcg gttgcagtgc
     4621 aggccggcgg attccgccag tgcggtgttc ggacggatgc cgaccgccat cacgaccagc
     4681 tcggcgggaa tctccagccc gtcggtaaa
//
LOCUS       DATLGF010000283         6031 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_58909,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000283 DATLGF010000000
VERSION     DATLGF010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6031
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58909"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /gene="kbl"
                     /locus_tag="VM406_08675"
     CDS             complement(<1..1191)
                     /gene="kbl"
                     /locus_tag="VM406_08675"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HVL76077.1"
                     /translation="MGSNARDSFYTGLEGQLAGLKDQGLYKPERVLASRQGAEVVADD
                     GRRLINMCANNYLGLSGSEDMVRAAMEATEQYGYGLSSVRFICGTQTVHKELERAIAD
                     FVGMEDAILYAAAFDANGGVFEPLLDENDAIISDALNHASIIDGIRLCKAARFRYRHN
                     DMDDLEAQLQAAAGKRHKLIVTDGVFSMDGTIAQLDKICDLADRYGALVMIDDCHATG
                     FMGRTGRGTHEHHDVMGRIDIITGTFGKALGGAMGGFTAARREVVQMLRQKSRPYLFS
                     NSLAPSIAGASLAVLKRLSASTELRDRLHENTTYFRKAIADLGFTIKPGTHAVVPVML
                     FDAPLAQRFAQRLYELGVLASGFFYPVVPMGQARVRVQMSAAHTSEHLDAALAAFAQA
                     GRELG"
     gene            1338..1910
                     /locus_tag="VM406_08680"
     CDS             1338..1910
                     /locus_tag="VM406_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HVL76078.1"
                     /translation="MKKPIQPSSPPEVGATLQKLRQKRGLTLDDLSRAAGVSKSMLSQ
                     IEREKANPTIAVTWRLANALGVGIEELLSSSNGDSEPVHVLEPHETPTLPGSHAGYTL
                     RILGPMELAGKTEWYELTLAAGGELRSHAHDPGTREHLTLISGSVEVEVDGVKKRLKP
                     GATARYQADRSHAICNTGKAEAKALLVVIH"
     gene            complement(2086..3408)
                     /locus_tag="VM406_08685"
     CDS             complement(2086..3408)
                     /locus_tag="VM406_08685"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HVL76079.1"
                     /translation="MKPIKVGLLGIGTVGSGTFNVLRRNREEITRRAGRSIEIAMVAD
                     LNVARAKELTNGECEVVNDANLVVNHPEIDIVIELIGGYGIAKDLVMKAIANGKHVVT
                     ANKALLATHGNEIFQAAQDKGVMVAFEAAVAGGIPIIKALREGLTANRIEWLAGIING
                     TTNFILSEMRDKGLDFDVVLKEAQRLGYAEADPTFDIEGVDAAHKATIMSAIAFGIPM
                     QFDKAHVEGITKLQATDIRYAEQLGYRIKLLGIARRTEVDGKAGIELRVHPTLIPAKR
                     LIANVEGAMNAVLVHADAVGATLYYGKGAGSEPTASAVIADLVDITRLATADPEHRVP
                     HLAFQPNALTDIPIVPMAEVTTSYYLRMRVADETGVLADVARILADMSISIDAMLQKE
                     PEEGETRTDIIMLTHQTKEKNVDAAIAKIEALPTVAGKVTRIRLEHLS"
     gene            complement(3483..4715)
                     /locus_tag="VM406_08690"
     CDS             complement(3483..4715)
                     /locus_tag="VM406_08690"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HVL76080.1"
                     /translation="MRPIQKSKKLADVCYDIRGPVLEKAKQMEEEGHKIMKLNIGNIA
                     VFGINPPDEIVHDMIINMGDAAGYTDSKGMFAPRKAVMHYTQEKKVPGVTIEDIYLGN
                     GASELIVMGMNALLNSGDEVLVPAPDYPLWTAAVSLSGGRPVHYVCDEASGWLPDIDD
                     IKKKVTPNTRAIVVINPNNPTGALYPVEVLEQIVEVARQHQLIVFADEIYDKTLYDGA
                     THTSIASLADDVLFVTLNGLSKNYRSCGYRAGWMVVSGEKRHAKDYIEGLNMLASMRL
                     CANAPGQFAIQTALGGYQSINDLVGPGGRLLKQRDLAHKLLTEIPGVTCVKPKAALYM
                     FPRLDPKMYPITDDQQFAYELLAEEKVLIVQGTGFNWIAPDHFRIVFLPNSDDLTEAI
                     TRIARFLDGYRRRYAAAA"
     gene            4898..5269
                     /locus_tag="VM406_08695"
     CDS             4898..5269
                     /locus_tag="VM406_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HVL76081.1"
                     /translation="MKLHSTPTQHYQTVTAYDDEGVEINAVRFRHSLIVTPEQAPSSW
                     APRAFEELTTDHFVQIGALDPDVVILGTGSRQRFVHPRLTAPLTDRRIGIECMDSQAA
                     CRTYNILMTEGRRVALALILE"
     gene            5313..5789
                     /locus_tag="VM406_08700"
     CDS             5313..5789
                     /locus_tag="VM406_08700"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HVL76082.1"
                     /translation="MAESAVALDQPVPDFSAPMTGDQTFQLSRLRGKTVVLYFYPKDN
                     TPGCTTEGMQFRDLYPEFQQANAELFGLSRDSIRSHEGFKAKLEMPFELISDPDEQVC
                     KLFGVMKNKQMYGKQVRGVERSTFVIDGSGKLVKEWRGVKVPGHVNEVLEFVKTLA"
BASE COUNT         1092 a         1879 c         1896 g         1164 t
ORIGIN      
        1 gccgagttcg cgcccggcct gtgcgaacgc cgccagcgcg gcgtcgaggt gttcgctggt
       61 atgcgcggcc gacatctgaa cgcgcacgcg cgcctgcccc atcggcacta ccgggtagaa
      121 gaaaccgctc gccagcacgc ccagctcgta caggcgctgc gcgaatcgct gcgccagcgg
      181 cgcgtcgaac agcatcaccg gcaccaccgc gtgcgtgccc ggcttgatcg tgaaacccag
      241 gtcggcgatg gccttgcgga agtaggtcgt gttctcatgc agacggtcgc gcagctcggt
      301 cgaggccgac aggcgcttga gcacggccag cgaagcgccg gcgatggagg gagcgagcga
      361 attcgagaac aggtaggggc gcgacttctg gcgcagcatc tgcaccactt cccgccgcgc
      421 cgcggtgaag ccgcccatcg cgccgcccag cgccttgccg aaggtgccgg tgatgatgtc
      481 gatgcgtccc atcacgtcgt ggtgctcgtg cgtgccgcgc ccggtcctgc ccatgaagcc
      541 ggtcgcatgg cagtcgtcga tcatcaccag cgcgccgtaa cggtcggcca ggtcgcagat
      601 tttgtcgagc tgggcgatcg tgccgtccat cgagaacacg ccgtcggtga cgatcagctt
      661 gtggcgcttg cccgcggccg cctgcagctg cgcctcgagg tcatccatgt cgttgtgccg
      721 gtagcgaaag cgcgccgcct tgcacaggcg gattccgtcg atgatggacg cgtggttgag
      781 cgcgtcggaa atgatcgcgt cgttctcgtc gagcagcggc tcgaacaccc cgccattggc
      841 gtcgaaggcg gcggcgtaaa ggatcgcgtc ctccatgccg acgaaatcgg cgatcgcgcg
      901 ctccagttcc ttgtgcacgg tctgggtgcc gcagatgaag cgcaccgacg acaggccgta
      961 gccgtattgc tcggtggcct ccatcgcggc gcgcaccatg tcctccgatc ccgacaggcc
     1021 gaggtagttg ttcgcgcaca tgttgatcag gcgccggccg tcatccgcca cgacttcggc
     1081 gccctggcgc gaggccagca cgcgctccgg tttgtagagg ccctggtcct tcaggccggc
     1141 cagctggcct tcgaggcccg tataaaagct gtcgcgcgca ttgctgccca ttcgatttct
     1201 ccgcctttgt gaggtgtccg tcccgcggcc atctgctacc atgcctgttc gacatgttgc
     1261 acaagttcga tatttgaaac gcttgttcga tatatcgaat cctagcgacg cctggtgaac
     1321 attgcaagcc tcctgatatg aaaaaaccca tccagccgag ttccccgccg gaagtcggcg
     1381 ccaccctcca aaaattgcgc cagaagcgcg ggctgacgct ggatgacctc tcacgcgcgg
     1441 ccggcgtgtc gaaatcaatg ctgtcgcaga tcgaacggga aaaggcgaac ccgaccatcg
     1501 cggtcacctg gcggctggca aacgcgttgg gagtcggcat cgaggaattg ctgtccagca
     1561 gcaatggcga cagcgagccg gtgcatgtgc tggagccgca tgagacgccg acgttacccg
     1621 gcagccatgc cggctataca ctgcgcatcc tcggcccgat ggaactggcg ggcaagaccg
     1681 aatggtatga gctgacgctg gccgctggcg gggagctgcg ctcgcatgcg cacgaccccg
     1741 gcacgcgcga gcatctcacc ctcatttcag gcagcgtcga ggtggaagtg gatggggtga
     1801 agaaaagact gaagccgggc gcgaccgcac gctaccaggc cgaccgcagc catgcgatct
     1861 gcaacaccgg gaaggcggag gcgaaagcgc tgctggtggt gattcactga ggcgatgcct
     1921 cgctggcctt tcagcccgtg cagtcggcgg cgctgcacgt ggggatgagg tccctcactg
     1981 cgttcgggac gacagggatt ctgccaatcg gagacgggga gctccggcac cggccttgcg
     2041 cgggctgcag gcactccccg tcctgaacgc ggtccggcga ccgcgtcagc tcaagtgctc
     2101 gaggcggatg cgcgtgacct ttccggccac cgtcggcagc gcctcgatct tcgcgatcgc
     2161 cgcatcgacg ttcttttcct tggtctgatg cgtgagcatg atgatgtcgg tgcgcgtctc
     2221 accctcctcc ggttccttct gcagcatcgc atcgatcgag atcgacatgt cggcaaggat
     2281 gcgcgcgacg tccgccagca cgccggtttc gtcggccaca cgcatgcgca ggtagtagct
     2341 ggtcgtgact tcggccatcg gcacgatcgg gatatcggtc agcgcattcg gctggaaagc
     2401 gaggtgcggc acgcgatgct ccggatcggc ggtcgccagg cgggtgatat cgaccaggtc
     2461 ggcgatcacc gcggatgcgg tcggctcgga acccgcgccc ttgccgtagt agagcgtggc
     2521 acccacggcg tcggcatgca cgagcaccgc gttcatcgcg ccttcgacgt tggcgatcag
     2581 acgcttggcg ggaatcagag tcggatgcac gcgcaattcg atgccggcct tgccgtcgac
     2641 ttcggtgcgg cgcgcgatcc cgagcagctt gatgcggtag ccgagctgtt cggcgtagcg
     2701 gatgtcggtc gcctgcagct tggtgatgcc ttccacgtgt gccttgtcaa actgcatcgg
     2761 gatgccgaaa gcaatcgccg acatgatggt cgccttgtgc gcggcgtcca cgccttcgat
     2821 atcgaaggtc ggatcggcct cggcgtagcc gagacgctgg gcctccttca gcaccacgtc
     2881 gaaatccagc cccttgtcgc gcatttccga gaggatgaag ttggtggtgc cgttgatgat
     2941 gcccgccagc cattcgatgc ggttggccgt gagcccttcg cgcagcgcct tgatgatcgg
     3001 aatgccgccg gcgaccgccg cttcgaatgc gaccatcacg cccttgtcct gggcggcctg
     3061 gaagatctcg ttgccgtgcg tggcgagcaa ggccttgttg gcggtgacga catgcttgcc
     3121 gttggcgatc gccttcatca cgaggtcctt cgcaatgccg tagccaccga tcagctcgat
     3181 cacgatgtcg atctcggggt gattcaccac cagattggcg tcattgacga cttcgcactc
     3241 gccgtttgtc agttccttgg cgcgcgccac attcaggtcg gccaccatcg cgatctcgat
     3301 gctgcgaccg gcgcggcggg taatttcctc gcggttgcga cgcaagacat tgaacgtgcc
     3361 ggaaccgacg gtgccgattc cgagtagacc tactttgatg ggtttcataa cgaaagactt
     3421 gggaagaaag tttggagcgt agcgctgcgt gccgccctcg ggcgacacgc agggcggcgg
     3481 gatcaggcag cggctgcgta tcgccggcgg tagccgtcga ggaagcgtgc aattcgggtg
     3541 atcgcctcgg taaggtcgtc cgaattgggc aggaaaacga tgcggaaatg gtccggggcg
     3601 atccagttga agccggtgcc ctgcacgatc aggaccttct cctcggccag caattcatag
     3661 gcgaactgct ggtcgtcggt gatcgggtac atcttgggat cgagccgcgg gaacatgtag
     3721 agcgcggcct tgggcttcac gcaggtcacg cccgggattt cggtcagcag cttgtgggcg
     3781 agatcgcgct gcttcagcag ccggccgcct ggcccgacga gatcgttgat gctctggtag
     3841 ccgcccagcg cggtctggat ggcgaactgt ccgggggcgt tggcgcaaag gcgcatcgaa
     3901 gcgagcatgt tcaggccttc gatgtaatcc ttcgcatgcc gcttctcgcc cgacaccacc
     3961 atccaaccgg cccgatagcc gcaggagcgg tagttcttcg agaggccgtt aagggtgacg
     4021 aacagcacgt cgtcggccag cgaggcgatc gaggtgtggg tcgcgccgtc gtagagggtc
     4081 ttgtcgtaga tctcgtcggc aaagacgatc agctgatgct ggcgcgcgac ctcgacaatc
     4141 tgctccagca cctcgaccgg atagagcgca ccggtcgggt tgttcgggtt gatgacgacg
     4201 atggcgcggg tgttcggcgt caccttcttc ttgatgtcgt cgatatccgg cagccagccc
     4261 gatgcttcat cgcaaacgta atggaccggc cgcccgcccg acaggctcac ggcggcggtc
     4321 cacagcgggt agtccggcgc gggcaccaac acttcatcgc cgctgttgag cagtgcgttc
     4381 atgcccatca cgatcagctc ggacgcgccg ttgccaagat aaatgtcctc gatcgttacg
     4441 ccggggacct tcttttcctg cgtgtaatgc atgacggcct tgcgcggcgc gaacatgccc
     4501 ttggagtcgg tgtagccggc ggcgtcgccc atgttgatga tcatgtcgtg cacgatctcg
     4561 tctggcggat tgatgccgaa cacagcaatg ttgccgatgt tcagcttcat gatcttgtgg
     4621 ccctcttcct ccatctgctt ggctttttcg aggacgggcc cgcggatgtc gtagcagaca
     4681 tcggccagct tcttggattt ttggataggt cgcacgctcg gtaccttgtt aattccttga
     4741 attgaaagcg gatttggcgg cgatgctgca ccgccaaaag aaccattttg cacaaagcaa
     4801 tctgtttaat caacggatta ggcctgcggc gttacaatga gcgttcaaat ttgccaagcg
     4861 ccggaaattc gagccccgcg cgtgtgtcta tttctgcatg aagcttcatt ccacgcccac
     4921 ccagcattac cagaccgtca ccgcctatga cgatgaaggc gtcgagatca acgcggtgcg
     4981 ctttcggcac agcctgatcg tcaccccgga acaggcgccc tcgtcatggg cgccccgtgc
     5041 tttcgaggaa ttgacgacgg accacttcgt ccagatcggc gccctcgatc ccgatgtggt
     5101 cattctcggc acaggcagcc gccagcgctt cgtccacccg cgactgaccg cgcccctcac
     5161 cgaccggcgc atcggcatcg aatgcatgga cagccaggcc gcatgccgga cgtacaacat
     5221 cctgatgacc gagggccgca gggttgcgct ggcactgatc ctcgaataac cgtatcgtct
     5281 gacccgaatt caaactgacc aggaggacct ctgtggcaga aagcgcagtt gcgttggacc
     5341 aacccgtacc cgacttctcc gccccgatga ccggcgacca gactttccag ctgtcccgct
     5401 tgcggggcaa gaccgtcgtt ctgtattttt atccgaagga caacacgccg ggctgcacca
     5461 ccgagggcat gcagtttcgc gacctctatc cggaattcca gcaggccaac gcggaactgt
     5521 tcggtctgag ccgcgacagc atccgctcac acgaaggctt caaggccaag ctggagatgc
     5581 cgttcgaact gatctccgat cccgacgagc aagtctgcaa gctgtttggc gtgatgaaga
     5641 acaagcagat gtacggcaag caggtgcgcg gcgtcgagcg cagtaccttt gtcattgacg
     5701 gctccgggaa attggtgaaa gaatggcgtg gagtgaaagt gcctggccac gtgaatgaag
     5761 tgctggagtt cgtcaagacg ctcgcctgag ctacggaatc aagaacgcat tgagtcacac
     5821 cgatcacccg tcgaaagccg tttgcggaag aggcctgatg cctcccccac aaacggcttt
     5881 tttgtttttt cacgccgtgt cgtccggcac gcggcgaccg agcagcgccc ccgcccttca
     5941 accttcccct tgaggttctg atgccactac ccaaattacc gaccaagcca gcaacattgc
     6001 tctccgttga agattatccg aaggcagaga a
//
LOCUS       DATLGF010000284         7084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_138468, whole genome shotgun sequence.
ACCESSION   DATLGF010000284 DATLGF010000000
VERSION     DATLGF010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138468"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VM406_08705"
     CDS             complement(<1..225)
                     /locus_tag="VM406_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HVL76083.1"
                     /translation="MTSTETSFPPLELSPDTPDCLNVAELIVAYLERLEVDYVFGVPG
                     GAIEPLYNALAASACRGGPRPVVARHETGAA"
     gene            complement(390..1610)
                     /locus_tag="VM406_08710"
     CDS             complement(390..1610)
                     /locus_tag="VM406_08710"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HVL76084.1"
                     /translation="MQTYVVGGAVRDDLLGLPVQDRDHVVVGATAEDMIARGFRPVGK
                     DFPVFLHPRTHEEYALARTERKTAPGYKGFVFHAAPDVTLEQDLARRDLTINAMARAE
                     DGGLVDPFGGLRDLEARVFRHVSDAFAEDPVRILRVARFAARFPDFTVASETVALMRR
                     MVEAGEVDALVAERVWQELARGLMEHRPSRMFDVLRSCGALERILPELDALWGVPQPA
                     QHHPEVDTGVHVMLVIDYAARQGYSLPVRFAALTHDLGKACTDPSQWPRHHGHEAHSE
                     RLVENVCKRLRVPNECRDLARMTAREHGNVGRALELRPNTIVKLLERCDALRKPQRFL
                     EMLRASECDRRGRPGFEDSAFPQFAHLEAALQAARAVDAGAIARLHAGEPQRIAEAVH
                     AARVDAVKARLFAA"
     gene            complement(1634..2347)
                     /locus_tag="VM406_08715"
     CDS             complement(1634..2347)
                     /locus_tag="VM406_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVL76085.1"
                     /translation="MQNTELDPALSRTLADAGKAGLKLVIGSKNYSSWSMRPWLALTA
                     FGIPFEEVRIPLGRPDTGARIAEYSAAGKVPVLLAGDLRIWDSLAICEYLAEQFPDKH
                     LWPRDVAARALARSICAEMHAGFTGLRSAMWMNITASFPGKGRTPAAQGDIARVCELW
                     EDCLERDGSGRFLFGEFSIADAYYAPVVMRFRTYGVWLAPALKAYVDRVVAHPAVARW
                     MQEAAAETDRLDKYDVYPD"
     gene            complement(2426..3394)
                     /locus_tag="VM406_08720"
     CDS             complement(2426..3394)
                     /locus_tag="VM406_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HVL76086.1"
                     /translation="MASNRFVLVLGGTGFIGSRIPADLVSTEWRVTVATRRADHARHL
                     LPLPSIEDIEEADVHDDAALDRLVQGQDAVINLVGILHGKRGPAGSRYGPAFARAHVD
                     LPRRVAAACARHGVRRLLHMSALGASANAPSMYFRSKHDGELAAQSQPAVAATIFRPS
                     VVFGPEDHLLNLFARLQRWLPVMLIGRPDARFQPVYVDDVARAFVRALEEPRSAGRIY
                     ELGGPRICTLRELVQMAGRYSGHPRPVIGLPDALAWLQALLMEWLPGGPLMSRDNLAS
                     LRVDTVTAATLREQFGIEPTPIEAVAPRYLRPLKATPRLPDMSQPA"
     gene            complement(3434..5329)
                     /locus_tag="VM406_08725"
     CDS             complement(3434..5329)
                     /locus_tag="VM406_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HVL76087.1"
                     /translation="MLVPGAYANPRSSALSSDDEVFLALREASRTGNATRAAELAGRL
                     GNYAIPSYVDYYRLRPRIHLASEMEIRDFLARYQGSAIADRLRNDWLIELGRARNWTL
                     FDEQYPQFVLNDDTQVRCYALMSRALRNQPVAGEARSLLVAPVNYGEGCTALVVTLVE
                     NRQFSADDAWAQVRRAADASQLHVLRRLAPATGVAEATLTQAYEKPDPVVTRGPGKDR
                     ASREVFILALGRAARANPQKAADALNKHAGRLSKSELAIAWSQVALQASLKLAPEAIE
                     YWRRTAGAPLSEDGYQWKARIALRSGDWTLVRDTIYAMPATLRTDATWIYWLGRAYAQ
                     EGKKEMAEKMYASIANQNSFYGKLALEELGRPITVPPRAAAPSAEELAPMAQNEGFKR
                     ALKFFDMNLRFEGIREWNWELRGMSERQHLAAAEFARQREVLDRMVSTSDRTRSEFDF
                     TQRFPAPFREVMVASTEKLGLDMAWVYGLIRQESRFILNARSHVGASGLMQLMPGTAQ
                     YVARKIGLGNFRPSQVNELETNITLGTNYLHMVLQDLDGSQALATAAYNAGPGRPRAW
                     RSTLPGQVEGAIFAESIPFSETRDYVKKVLSNATYYAALFEDKPQSLKARLGMVAPRD
                     FVPSELP"
     gene            5371..6081
                     /locus_tag="VM406_08730"
     CDS             5371..6081
                     /locus_tag="VM406_08730"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HVL76088.1"
                     /translation="MKTSRLLPSLWSETSSCRIAWWSWGKTPNWHAYGAVFHGREQAR
                     LTGCKPYMQEKTDKAALRRALLANRQQLPEEVRKLLDARIGAQVLAWWRMQRPQRIGV
                     YWPMRAEPDLREIYTTLCREGVQLALPVVIEKDVALDFAAWKPGAALVRDCHGVPVPP
                     TGAARLKPDALLIPCVGFNAKRQRLGYGGGYYDRTLAGTPRPETIGVAYDIARCDFEG
                     GEHDIALDRIITESGLLP"
     gene            complement(6064..6912)
                     /gene="metF"
                     /locus_tag="VM406_08735"
     CDS             complement(6064..6912)
                     /gene="metF"
                     /locus_tag="VM406_08735"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HVL76089.1"
                     /translation="MSPSNFSIEFFPPKTTEGVEKLRTVRAKLAALRPQYFSVTFGAG
                     GSTQQGTLDTVLEIQREGLEAAPHISCMGNSRENLRALLEQYKAAGIRRLVALRGDLP
                     SGYGALGMSDNQFRYANELIEFIRAETGDWFHIEVAAYPEMHPQATSPQEDLRNFARK
                     VAAGADAAITQYFYNADAYFRFVDDARKAGVKVPIVAGIMPITNYSQLVRFSDMCGAE
                     IPRWIRLKLAGFGDDTASIKAFGLDVVSAMCERLLANGAPGLHFYTLNQSAPTMEICA
                     RLGQKS"
     gene            complement(6915..>7084)
                     /locus_tag="VM406_08740"
     CDS             complement(6915..>7084)
                     /locus_tag="VM406_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HVL76090.1"
                     /translation="TVVTLGLFIFVINALLFWMVANFVSGFHVAGFWSALGGAILYSL
                     ISWALSSLIMK"
BASE COUNT         1271 a         2331 c         2321 g         1161 t
ORIGIN      
        1 tgccgcgccg gtttcatggc gcgcgaccac cgggcgcggt ccgccgcgac atgcgcttgc
       61 cgccagtgcg ttgtacaggg gttcgatcgc accgccgggc accccgaaga cataatcaac
      121 ttccagtctc tcaaggtaag caactatcag ttccgccaca ttgaggcagt cgggggtgtc
      181 gggagagagt tcgagcggag ggaaagaggt ctcggtgctg gtcacagcga tactttcagt
      241 ggtccgcgac agcggactgc ctcgcttccg tgctgccttg ctgttgcgaa tgttcaggtg
      301 gcggcaatag taccgtaatt gcgcgaatgc aatcgaagct ttttcggttc cgttacacga
      361 ggggacgatg gcgcggcacg gccgcaagtt caggctgcga acaggcgcgc cttgacggca
      421 tccacgcggg ccgcatggac cgcctcggcg atgcgctgcg gctcgccggc gtgaagccgc
      481 gcgatggcgc cggcatcgac cgcgcgcgcc gcctgcagcg ccgcttccag gtgcgcgaac
      541 tgcggaaacg cggaatcctc gaaaccgggg cgcccgcgcc ggtcgcactc ggacgcgcgc
      601 agcatctcga ggaaacgctg cggcttgcgc aaggcgtcgc agcgctcaag cagcttgacg
      661 atggtgttcg ggcgcagttc gagcgcccgc ccgacattgc cgtgctcgcg cgcggtcatg
      721 cgcgcaaggt cgcgacattc gttcgggacg cgcagacgct tgcacacgtt ttcgaccagg
      781 cgctcgctat gcgcttcgtg gccatggtgg cgcggccatt ggctcggatc ggtacacgcc
      841 ttcccaaggt catgcgtcag ggccgcgaaa cgcaccggca ggctgtagcc ctggcgcgcg
      901 gcgtagtcga tcaccagcat cacatgcacg ccggtgtcga cctcgggatg gtgctgtgcc
      961 ggttgcggaa cgccccacag tgcgtcgagc tcgggaagaa tgcgttccag cgcgccgcag
     1021 ctgcgcagca catcgaacat gcgcgacgga cgatgctcca tcagcccgcg cgcgagttcc
     1081 tgccagacgc gctcggccac cagcgcatcg acctcgccgg cctcgaccat gcgccgcatc
     1141 agcgcgacag tctccgaggc gacagtgaag tccggaaaac gcgcggcgaa acgcgccacg
     1201 cgcaggatgc gcaccggatc ttcggcgaag gcgtccgaca catggcggaa gacgcgagcc
     1261 tcgagatcgc gcagaccgcc gaacggatcg accaggccgc cgtcctcggc gcgcgccatc
     1321 gcattgatgg tgaggtcgcg gcgcgccaga tcctgctcca gcgtcacgtc cggcgccgca
     1381 tggaacacga agcccttgta gccgggcgcg gtcttgcgct cggtgcgcgc caacgcatat
     1441 tcctcgtggg tgcgcggatg caggaaaacg ggaaagtcct tgccgaccgg gcgaaagccg
     1501 cgcgcgatca tgtcctcggc tgttgcaccg accaccacat ggtcgcggtc ctgcaccggc
     1561 aggccgagca gatcgtcgcg caccgcaccg ccaacgacat atgtctgcat gggatggaat
     1621 ttaatctgcg caatcagtcc ggatagacat cgtatttgtc cagtcgatcc gtctcggccg
     1681 cggcttcctg catccagcgc gccaccgccg ggtgagcgac cacgcgatcc acgtaggcct
     1741 tcagcgccgg cgccagccac acgccatagg ttctgaaacg catgacgacc ggcgcgtaat
     1801 acgcatcggc gatcgagaac tcgccgaaca ggaagcggcc cgacccgtcg cgctcgaggc
     1861 aatcctccca gagctcgcac accctggcga tgtcgccctg ggcggcaggt gtgcgcccct
     1921 tgccgggaaa gctggcggtg atgttcatcc acattgccga acgcagcccg gtgaagcccg
     1981 cgtgcatttc cgcgcagatc gaacgcgcca gcgcgcgggc ggccacatcg cgcggccaca
     2041 ggtgcttgtc ggggaattgc tcggcgaggt attcgcagat ggcaagtgag tcccaaatcc
     2101 tgagatcgcc ggccagcagc accggcacct tgcctgcggc agagtactcg gcgatgcgcg
     2161 caccggtgtc cggacgacct agcggaatgc gcacctcctc gaacggaatg ccgaaagcgg
     2221 tgagcgccag ccaaggccgc atcgaccacg acgaatagtt cttgctgcca atgacgagct
     2281 tcaggccggc cttgccggca tcggcaagcg tgcggctcag cgccggatcg agttcggtgt
     2341 tctgcatatt ggttcggatg gtggtacgcg gcacgcccgc aaccgtccga gcatatcggc
     2401 ttttttccct cttgcaaacc gcgcgtcagg ccggctgcga catgtccggc aggcgcggcg
     2461 tcgccttcag cggccgcagg tagcgcggcg cgacggcctc gatcggtgtc ggttcgatgc
     2521 cgaactgctc gcgcagcgtg gccgcggtca cggtatccac acgcagcgaa gccaggttgt
     2581 cgcggctcat caacggcccg cccggcagcc actccatcag cagagcctgc agccacgcca
     2641 acgcatcggg cagaccgatc accggccgtg gatgaccgga atagcggccg gccatctgca
     2701 ccagctcgcg cagtgtgcag attcgcggac cgccaagctc gtagatccgc ccggcactgc
     2761 gcggctcctc gagcgcgcgc acgaaggctc gcgccacgtc atccacatag accggctgga
     2821 aacgcgcgtc cgggcgtccg atcaacatca ccggcagcca gcgctgcagc cgcgcgaaca
     2881 aattgagcag atggtcttcg ggaccgaaca ccaccgaagg gcggaagatc gtggcagcaa
     2941 ccgcgggctg gctctgcgcg gccagctcgc cgtcgtgctt ggaacggaaa tacatcgacg
     3001 gcgcattggc ggacgcgccg agcgcgctca tgtgcaacaa ccggcgcacc ccatgccggg
     3061 cacaggcagc ggccacgcgg cgcggcaggt ccacatgggc acgggcgaat gccggaccat
     3121 agcgcgagcc tgcgggtccg cgctttccgt gaaggatgcc gacgaggttg atcaccgcat
     3181 cctgtccctg caccagccgg tcgagcgccg catcgtcgtg cacgtcggct tcctcgatgt
     3241 cttcgatcga gggcagcggc aacaggtggc gcgcatgatc ggcgcggcgc gtggccaccg
     3301 tcacgcgcca ctcggtggat accagatcgg cggggatccg gctgccgatg aaaccggtgc
     3361 cgccgagaac cagaacgaaa cgatttgaag ccatgatgac ctccgtacgc cctgcttcgc
     3421 ggcaatcact ccatcagggc aactcgctcg gaacgaaatc gcgcggcgcc accatgccca
     3481 gccttgcctt cagcgactgc ggtttgtcct cgaacagcgc cgcgtagtac gtcgcattgg
     3541 acagcacctt cttcacatag tcgcgcgttt cggaaaaggg aatcgattcc gcgaagatcg
     3601 ccccctcgac ctgcccgggc aaggtcgaac gccatgcgcg cggacgcccc ggacctgcat
     3661 tgtaggccgc ggtggccagc gcctgcgagc cgtccagatc ctgcaacacc atgtgcaggt
     3721 aattggtgcc gagagtgatg ttggtttcca gctcgttgac ctggctcggc ctgaagttgc
     3781 ccaggccgat cttgcgggcg acatactgcg cggtgcccgg catcagctgc atcaggccgg
     3841 aagcgccgac atgcgagcgc gcattgagaa tgaaacgcga ttcctgccgg atcaggccat
     3901 acacccaggc catgtccagc cccagctttt cggtgctggc gaccatgacc tcgcggaacg
     3961 gcgccggaaa acgctgcgtg aaatcgaact cggagcgcgt gcggtccgac gtgctcacca
     4021 tgcgatcgag cacttcgcgc tgacgcgcga actcggccgc cgccaggtgc tggcgctcgc
     4081 tcatgccgcg cagctcccag ttccattcgc ggatgccttc gaagcgcagg ttcatgtcga
     4141 agaatttcag cgcgcgcttg aacccctcgt tctgcgccat cggcgcgagc tcctccgcgg
     4201 agggcgcggc agcgcgcggc ggcaccgtga tcggacgccc cagctcctcg agcgcaagct
     4261 tgccgtagaa ggaattctgg ttggcgatcg acgcgtacat cttttccgcc atctccttct
     4321 tgccttcctg cgcatacgcg cggccgagcc agtagatcca ggtggcgtcg gtgcgcagcg
     4381 tggccggcat cgcgtatatc gtgtcacgca ccagcgtcca gtcgcccgag cgcagcgcga
     4441 tgcgcgcctt ccactggtag ccgtcctcgg acaagggcgc gccggcagtg cggcgccagt
     4501 actcgatcgc ttccggcgcc agtttcagcg acgcctgcag cgcgacctgc gaccaggcga
     4561 tcgccagctc ggacttggag agacggccgg cgtgcttgtt cagcgcatcg gccgctttct
     4621 gcgggttcgc gcgcgcagcg cggcccaagg cgagaatgaa cacttcgcgc gatgcgcggt
     4681 ccttccccgg tccacgggtg acaaccgggt ccggtttctc gtacgcctgc gtgagcgtgg
     4741 cttcggccac gccggtggcc ggcgcgaggc ggcgcaatac gtggagttgg ctggcgtcgg
     4801 ccgcgcggcg cacctgcgcc caggcgtcgt ctgcgctgaa ctgccgattc tcgaccaggg
     4861 tgacgaccag cgcggtgcag ccttcgccat agttcaccgg cgcaaccagc aggctgcgcg
     4921 cctcgccggc cacaggctgg ttgcgcaacg cccgcgacat cagcgcgtag cagcggactt
     4981 gggtatcgtc attcagaaca aactgcgggt actgctcgtc gaacagggtc caattacgcg
     5041 cgcggcccag ctcgatcagc cagtcgttgc gcagccggtc ggcgatcgcg ctgccctggt
     5101 agcgtgccag aaagtcgcgg atttccatct cggaagcgag atggatgcgc ggacgcagac
     5161 ggtagtagtc gacgtaggaa ggaatcgcgt aattaccgag gcggccggcg agttcggcgg
     5221 cccgggtcgc gttgccggtg cgggatgcct cgcgcagcgc aagaaaaact tcgtcatcgc
     5281 tcgaaagcgc gctgctgcgg gggttcgcat aggcaccggg cacgagcatg ccggctgccg
     5341 ctgcgagtgc aatcccggca gcgcatctgt atgaaaacgt cacgacttct accttccctt
     5401 tggagcgaaa cttcgagttg tagaatagca tggtggtcct ggggcaaaac tccgaactgg
     5461 catgcttacg gcgcagtttt tcatggccgc gaacaggcgc gtttgacagg ctgcaaacca
     5521 tacatgcaag aaaaaacaga caaggcggca ttgcgccgcg cgttgcttgc caacagacaa
     5581 caactccccg aggaagttcg gaaacttttg gatgcacgca tcggtgcgca ggtactcgcg
     5641 tggtggcgca tgcagcggcc gcaacgcatc ggcgtgtact ggccaatgcg cgcggagccg
     5701 gacctgcgcg agatctatac gacgctatgc agggaaggcg tgcagcttgc gctgccggtg
     5761 gtaatcgaga aggatgtggc gctggatttt gcggcctgga agcctggcgc tgcgctggtg
     5821 cgggactgcc acggcgtacc ggtcccgccg acgggcgcag cgcggctgaa accggatgcg
     5881 ctgctcattc cctgcgtggg cttcaacgcg aagcgtcagc gtctgggcta tggcggcggc
     5941 tactacgacc gcacactggc cgggacaccg cgcccggaaa caattggcgt tgcctacgac
     6001 atcgcccgct gcgacttcga aggcggcgag cacgacatcg cgctcgaccg catcatcacc
     6061 gaatcaggac ttttgcccta gccgcgcgca gatttccatc gtcggcgcgg actggttgag
     6121 cgtgtagaag tgcagccccg gcgcgccgtt ggcgagcagg cgctcgcaca tcgcggacac
     6181 cacatccagc ccgaatgcct tgatcgacgc ggtgtcgtcg ccgaagccgg ccagcttcag
     6241 ccgtatccag cgcgggatct ccgccccgca catgtcggaa aaacgcacca gctgcgagta
     6301 gttcgtgatc ggcatgatgc cggccacgat cggcaccttc acaccggcct tgcgcgcatc
     6361 gtcgacgaag cggaagtagg cgtcggcgtt gtagaagtac tgcgtgatcg ccgcgtcggc
     6421 gccggctgca accttgcgcg cgaaattcct caggtcttcc tgcggcgagg tcgcctgcgg
     6481 atgcatctcg ggataggcgg cgacttcgat atggaaccag tcgccggtct cggcgcggat
     6541 gaactcgatc agttcgttcg cgtagcggaa ctggttgtcc gacatcccca gcgcgccgta
     6601 gccgctcggc agatcgccgc gcagggcgac caggcggcgg attccagcgg ccttgtactg
     6661 ctcgagcagc gcgcgcaggt tttcgcgcga attgcccatg caggagatgt ggggcgcggc
     6721 ttcgagacct tcgcgctgga tttcgagcac ggtatcgagc gtgccctgct gggtggaacc
     6781 gcctgcgccg aaggtcacgg agaagtattg cggacgcagc gcggcaagtt tcgcgcgcac
     6841 cgtgcgcagc ttctccacgc cctcggtcgt cttcggcggg aagaactcga tgctgaaatt
     6901 gctggggctc atgtttattt cattatcagg gacgacagcg cccaggaaat gaggctgtac
     6961 aggatcgcgc cgccaagcgc cgaccagaag ccggccacgt ggaaaccgga gacgaagttc
     7021 gcgaccatcc agaacagcaa ggcgttgatc acgaagatga acaggcccag cgtgaccacg
     7081 gtga
//
LOCUS       DATLGF010000285         6542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_169135, whole genome shotgun sequence.
ACCESSION   DATLGF010000285 DATLGF010000000
VERSION     DATLGF010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6542
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169135"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /gene="hemA"
                     /locus_tag="VM406_08745"
     CDS             <1..1158
                     /gene="hemA"
                     /locus_tag="VM406_08745"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HVL76091.1"
                     /translation="GEASPEAAILSTCNRTELYAANPADGAVDTAIDATAHFLADYHK
                     LPYGELRPYLYTLPQDNAVRHAFRVASGLDSMVLGEAQILGQMKEAVRQADEAGGLGT
                     YLHQMFQRTFAVAKEVRSTTEIGAHSVSMAAAAVRLSQRIFESIAQQHVLFIGAGEMI
                     ELCATHFAAQNPKSLTIANRTLERGEALAHRFNGRAMRLVDLPQQLAQFDIVVSSTGS
                     SLPIIGLGMVERAIKARRHRPMFMVDLAVPRDIEAEVARLDDVFLYTVDDLGAAVQTG
                     IENRQAAVAQAEAIIETRVQSFMHWVDSRSVVPVIRDLHDSGEAMRLAELERARRMLA
                     RGEDIETVLDALSKGLTAKFLHGPQQALHNAQGDERARLASLLPHLFRTKR"
     gene            1228..2310
                     /gene="prfA"
                     /locus_tag="VM406_08750"
     CDS             1228..2310
                     /gene="prfA"
                     /locus_tag="VM406_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HVL76092.1"
                     /translation="MKPSMLAKLNQLTERLEELNHLLAQEGVTSDIDQYRKLTREHAE
                     LEPLVGLYKNYVQAGRDIQAAQDMLADPDMKELAQEEITDARARIEQYEVDLQKMLLP
                     KDPNDERNIFLEIRAGTGGDEAALFAGDLLRMYTRYAERNRWQVEMVSESPSEIGGYK
                     EVIVRVIGFGAYSKLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADEVSDVDIN
                     PADIRIDTFRASGAGGQHINKTDSAVRITHIPTGIVVECQDDRSQHKNKANALKVLAA
                     RIKDIQMREQQQKEAATRKSLIGSGDRSERIRTYNFPQGRITDHRINLTLYKLDFVMD
                     GDLDELTNALIAEHQAEQLAALAEEA"
     gene            2374..2847
                     /locus_tag="VM406_08755"
     CDS             2374..2847
                     /locus_tag="VM406_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HVL76093.1"
                     /translation="MKSSKPVLLAIAAVSLGLVGYALYLQHQLGWAPCPLCVIQRYVF
                     VGVAAVCLLGALLPRAAAPVGAGLGALVALGGAGVASWHTWVKANPTVSCGIDPLETA
                     LNRIPPADWMPFLFKADGLCATPYPPILGLQVPHWSLIWFAIFALVLGWAAIRAR"
     gene            2852..3691
                     /gene="prmC"
                     /locus_tag="VM406_08760"
     CDS             2852..3691
                     /gene="prmC"
                     /locus_tag="VM406_08760"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HVL76094.1"
                     /translation="MTRPAFSLPANTTIASALRQLPLDALENRILLMHALGLSRVELI
                     TQSERVISDDEAQRLSALIERRLAGEPIAYIVGEREFFGLALHVTPDVLIPRPDTELL
                     VELALDRLPHGGRVVDLGTGSGAIAVAVAHQRPDAEVHAVDISAAALAVAGRNAERHA
                     VRLHLHQGDWLSPLSGEPFDLVVSNPPYIRSDDPHLAQGDLRFEPIGALTDHGGGLSA
                     LRNIATSAMHHLKPGSWLLMEHGYDQASDVRRMLAGSGYTAVQSWHDLAGIERVTGGR
                     LSR"
     gene            3881..6004
                     /locus_tag="VM406_08765"
     CDS             3881..6004
                     /locus_tag="VM406_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVL76095.1"
                     /translation="MIFREHVIRLHITISLLFVVLTVPVMATFVIVSYRANLQLSADY
                     SEKFIEKSLADTVSVTTQLFSPMLTTVRTAAALMRDQPDYFRQESSAEYLNEILALNE
                     AVYAAYVSFEDGSFRQVRRAISGSAVLGVAPPQQTTVVSRFIDARAKGAATDRYIFLA
                     GWGEPVGSHAGPASYDPRVRGFYRDSVRLKAANISDPYLFASSGELGITVSAPVIARD
                     RVLGVVAGDFTLKTLSRHLLDNRVSPNSTTIISDVAGGIVAHPDFSQALARQDGELVR
                     ARLDRLPDPRVRAALAERLRSGAERFKFRAGPDNVEYLGIFSPFPTSFNKEWEMLIIA
                     PTDDFIGQIRRTNRNLLIFGLAALLLQILLIRRLARSVARPIEKLADEVTHIREFRFD
                     KVGTVNSQVHEIRCMAEAVTLLGRSLESFTSYVPRGLVQQLVKSGRSTALGVQSRYLT
                     ILFTDIEGFSSLAEAEPSQQLLARVSEYFSVMTQAIEKEHGTVDKFIGDAVMAFWGAP
                     ATLDDHAYRACVAAVRAQRAMAARNSAWIAQNIAPLRVRIGIHADSALVGNVGSVERV
                     SYSVMGDGVNVAARLEGINKEMGTWTCVSHAVYREAGERLWLRPIDSVTVKGRKGELV
                     VYELLGIRDIPELAANAEEIALCELTSLAYAQYAQGKLAAAANAYQAILERFPEDPVA
                     RRMRDKCRAQGPRTAQALAAPADAI"
     gene            6029..>6542
                     /locus_tag="VM406_08770"
     CDS             6029..>6542
                     /locus_tag="VM406_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HVL76096.1"
                     /translation="MPRLRRLIHAFAALALLLAAGTGIARDMAAIKSSGTLVVAVPDM
                     RNPPFFFEDGGELKGLDIDLAKSIAESLGVQVAFNRDAKNFNDAVQLVALGRADMAAA
                     KISRTLARAQTVLFTDPYLVLPHALLLNRLRFAEISRGRAPADVLKNFTGSVGVIANS
                     SFVGFAKTHFP"
BASE COUNT         1132 a         2280 c         2037 g         1093 t
ORIGIN      
        1 ggcgaggcct cgcccgaggc tgcgatcctg tcgacttgca accgcaccga actgtacgcg
       61 gccaatccgg ccgatggcgc ggttgacacg gccatcgacg cgaccgccca cttcctggcg
      121 gattaccaca agctgccgta cggcgaactg cgcccctatc tgtacacgct gccgcaggac
      181 aatgcagtgc gccacgcatt ccgcgtcgcc tccgggctcg attcgatggt gctgggcgag
      241 gcgcagatcc tcggccagat gaaagaagcg gtgcgccagg ccgacgaggc aggcggactg
      301 ggcacttatc tgcaccagat gttccagcgc accttcgcgg tggcgaagga agtgcgcagc
      361 acgaccgaga tcggcgcgca cagcgtatcg atggcggccg cggccgtgcg actgtcgcaa
      421 cgcatcttcg aaagcatcgc acagcagcat gtgctgttca tcggcgcggg cgaaatgatc
      481 gagctgtgcg cgacccactt cgccgcgcag aatccgaagt cgctgaccat cgccaaccgc
      541 acgctggagc gtggcgaggc gctggcccat cgcttcaacg ggcgcgcgat gcggctggtt
      601 gacctgccgc agcagctcgc gcagttcgac atcgtggtgt cctccaccgg ctcctcgctg
      661 ccgatcatcg gactcggcat ggtggaacgc gcgatcaagg cgcgccgcca tcgcccgatg
      721 ttcatggtcg atctggcggt accgcgcgac atcgaagccg aagtcgcgcg gctggacgat
      781 gtgttcctat acacggtcga cgacctcggc gcggcggtcc agaccggcat cgagaaccgc
      841 caggccgcgg tggcgcaggc cgaagcgatc atcgagaccc gcgtgcagtc gttcatgcac
      901 tgggtcgaca gccgttccgt cgtgccggtg atccgcgatc tgcacgacag cggcgaggcg
      961 atgcggctgg cggaactcga gcgcgcccgg cgcatgctgg cgcgcgggga agacatcgag
     1021 accgtgctcg acgccctctc caagggtctg accgcgaaat tcctccatgg cccgcagcag
     1081 gcactgcaca acgcacaggg cgacgaacgc gcgcgcctgg cctcgctgct gccgcatctg
     1141 ttccgcacca agcgctagcg gatcacccgc ctgtcgtgcc gggcgcggcc atgcgccccg
     1201 ttgttccctc cttacctgtc cgcccccatg aaaccttcga tgctcgccaa actgaaccag
     1261 ctcaccgaac gcctggagga gctgaaccac ctgctcgcgc aggagggcgt cacgagcgac
     1321 atcgaccagt accgcaagct cacccgcgag catgcggagc tggagccgct ggtgggcttg
     1381 tacaagaact atgtgcaggc tggccgggat atccaggccg cccaggacat gctggccgac
     1441 cccgacatga aggagctcgc gcaggaagag atcaccgatg cccgcgcgcg catcgaacag
     1501 tacgaggtcg acctgcagaa aatgctgctg cccaaggatc ccaacgacga gcgcaacatc
     1561 ttcctcgaaa tccgcgccgg caccggtggc gacgaagcgg cgctttttgc cggcgatctg
     1621 ctgcgcatgt acacccgcta cgcggaacgc aatcgctggc aggtggaaat ggtgtcggag
     1681 tcgccatcgg aaatcggtgg ctacaaggaa gtgatcgtgc gcgtgatcgg cttcggcgcg
     1741 tattcaaagc tcaagttcga atcgggcggc catcgcgtgc agcgtgtgcc ggcgaccgaa
     1801 acccagggcc gcatccatac ctcggcctgc acggtggcgg tgatgccgga ggccgacgag
     1861 gtcagcgacg tcgacatcaa cccggccgac atccgcatcg acaccttccg cgcctcgggc
     1921 gccggcggcc agcacatcaa caagactgat tcggcggtgc gcatcacgca catcccgacc
     1981 ggcatcgtgg tggagtgcca ggacgaccgc agccagcaca agaacaaggc caatgcgctg
     2041 aaggtgctgg ccgcgcgcat caaggacata cagatgcgcg agcagcaaca gaaggaagcg
     2101 gccacgcgca agtcgctgat cggctcaggc gatcgcagcg agcgcatccg cacctacaac
     2161 ttcccgcagg gccgcatcac cgatcaccgc atcaacctga cgctgtacaa gctggacttc
     2221 gtgatggacg gcgatctcga cgaactgacc aatgcgctga ttgccgagca ccaggccgag
     2281 caattggccg cattggctga agaggcgtaa catcttgcca accggaacca aacgcccttc
     2341 ccgcactcct accggctcgc accgatcctt gccatgaaaa gctcaaaacc cgtcctgctc
     2401 gccatcgccg ccgtctcgct cggactggtc ggctacgctc tctatctcca gcatcagctc
     2461 ggctgggcgc cctgtccgct gtgcgtgatc cagcgctatg tgttcgtcgg cgtggccgcg
     2521 gtctgcttgc tcggcgcact gctgccgcgc gccgccgcgc cagtcggggc gggcctcggg
     2581 gctctcgtcg ctctgggcgg cgcaggcgtt gcgagctggc acacctgggt gaaggccaat
     2641 cccaccgtgt cctgcggcat cgatccgctg gaaacggcgc tcaaccgcat tcctcccgcc
     2701 gactggatgc ccttcctgtt caaggccgac ggcctgtgcg caacgcccta tccgccgatt
     2761 ctcggcctgc aggtcccgca ctggtcgctg atctggttcg ccatcttcgc gctcgtgctc
     2821 ggctgggcag ccatccgcgc gcgctgatgc gatgacacgg cccgcgttct cgctgccggc
     2881 caacacgacg atcgcctccg cgctcaggca gcttccgctc gacgcgctgg agaaccgcat
     2941 cctgctcatg catgcgctcg ggctgtcccg cgtcgaactg ataacgcaat cggagcgagt
     3001 catctccgac gacgaagcgc aacgcctctc tgccctgatc gaacgtcggc tcgcgggtga
     3061 accgattgcc tacatcgtcg gcgagcgtga gttcttcggt cttgccctgc acgtcacccc
     3121 cgacgtgctg attccgcgcc cggacaccga gctgctggtc gaactggcgc tggaccgcct
     3181 gccgcatggc gggcgcgtgg tcgatctcgg caccggctcg ggagcgattg ccgtggccgt
     3241 cgcccaccaa cgtcccgatg ccgaggtgca cgcggtggac atcagcgccg ccgcgctcgc
     3301 agtcgcaggc agaaatgccg aacgccatgc ggtacgtctt cacctgcacc aaggcgactg
     3361 gctctcgccg ttatcgggcg agccgttcga cttggtcgtc tccaacccgc cgtacatccg
     3421 ctcggacgac ccccacctcg cacagggcga cctacgcttc gagccgatcg gtgcgctgac
     3481 cgaccatggc gggggactgt cggcgttgcg caacatcgcc accagcgcca tgcatcatct
     3541 aaagcccggc agctggctgc tgatggaaca cggctacgac caggcctccg acgtgcggcg
     3601 catgctcgcg ggcagcggat atacggccgt acagagctgg catgacctgg ctggcatcga
     3661 gcgcgtcacc ggcggccgcc tgtctcgctg acccggcccc ggcgccatcc tccgtcccgc
     3721 ctgcccgcgt tgccgcggaa gaatttcctt aatcaaaatc aaacagtcgt ctaccataga
     3781 gacattttta taaggaaatt ctcctggaag gcatcgctgg ctccacttcg ccgccgttcg
     3841 ccccgcgcac gatcggcctc cttccggcag gtttgggacc atgatctttc gcgaacacgt
     3901 catccggctt cacatcacga tcagcctgct gttcgtcgta ctgaccgtgc cggtgatggc
     3961 gaccttcgtc atcgtcagct accgcgccaa cctgcagctg agcgccgact attcagagaa
     4021 gttcatcgag aaatcgctgg cggacacagt cagcgtgacg acccagctgt tcagtccgat
     4081 gctgacaacc gtgcgcaccg cggcggcttt gatgcgcgac cagccggatt atttccgcca
     4141 ggagtcctcg gccgagtacc tgaacgaaat cctggcgctg aacgaggctg tctacgccgc
     4201 ctatgtcagc ttcgaggatg gctcgttccg tcaggtgcgc cgcgcgatct ccggcagtgc
     4261 ggtgctcggg gtcgcgcccc cgcagcagac caccgtggtc agccgcttca tcgacgcgcg
     4321 agcgaagggt gcggcaaccg accgctatat cttcctcgct ggctggggcg aacccgtcgg
     4381 cagccatgcc gggcccgcca gctacgaccc gcgcgtgcgc ggcttctatc gcgactcggt
     4441 gcggctcaag gccgccaaca tctccgatcc gtatctgttc gcgagctcgg gcgaactcgg
     4501 catcaccgtc agcgctccgg tgatcgcgcg cgaccgggta ctcggggtcg tggccggcga
     4561 tttcacgctg aagacgctgt cgcgccacct gctggacaac cgcgtctcgc ccaacagcac
     4621 caccatcatc tccgacgtgg cgggcggcat cgtcgcacac ccggatttct cgcaggcgct
     4681 tgcgcgccag gacggcgagc tggtgcgcgc ccgcctcgac cgcctgcccg atcctcgggt
     4741 gcgcgccgcg ctcgccgagc gtctgcgcag cggcgctgag cgtttcaagt tccgcgccgg
     4801 gccggacaat gtcgaatacc tcggcatctt ttcccccttc cctacgagct tcaacaagga
     4861 atgggagatg ctgatcattg cgccgaccga cgacttcatc ggccagatcc gccgcaccaa
     4921 ccgcaatctg ctgatcttcg gactggctgc gctgctgctg cagatcctgc tgatccgccg
     4981 cctggcgcgc tcggtcgcgc gaccgatcga aaagcttgcc gacgaggtga cccacatccg
     5041 cgagttccgc tttgacaagg tcggcacggt gaattcgcag gtgcacgaga tccgttgcat
     5101 ggccgaagcg gtcacgctgc ttgggcgcag cctcgagtcg ttcacctcct acgtgccgcg
     5161 cggcctcgtc caacagctcg tcaagagcgg gcgcagcacc gcgctgggcg tgcagagccg
     5221 ctacctgaca atcctcttca ccgacatcga gggcttctcc agcctggctg aagccgaacc
     5281 ctcgcaacag ttgctggcac gcgtatccga gtatttctcg gtgatgacgc aggcgatcga
     5341 gaaggagcac ggtacggtcg acaagttcat cggcgacgcc gtgatggcct tctggggcgc
     5401 gccggcgacg ctggacgacc acgcctaccg cgcatgcgtg gcggcggtgc gggcgcagcg
     5461 cgcgatggcc gcgcgcaaca gcgcttggat cgcgcagaac attgctccgc tgcgcgtgcg
     5521 catcggcatc catgccgatt ccgcgctggt gggcaatgtc ggctcggtcg agcgcgtcag
     5581 ttattccgtg atgggagacg gcgtgaacgt cgcggcgcgg ctcgaaggca tcaacaagga
     5641 gatgggcacc tggacctgcg tgagccacgc cgtctaccgc gaggcgggcg aacgcctgtg
     5701 gctgcgcccg attgacagcg tcaccgtcaa gggccgcaag ggggagctgg tggtctacga
     5761 gctgctcggt atccgcgaca ttccggaact ggcggcgaac gccgaggaga tcgcgctgtg
     5821 cgagctcacc agccttgcct atgcgcagta cgcgcaggga aagcttgccg ctgccgcaaa
     5881 cgcctatcag gccatcctcg aacgctttcc cgaggacccg gtcgcgcgtc gcatgcgcga
     5941 caagtgccgc gcgcaaggtc cgcgcacggc gcaggcgctc gccgctcctg ccgacgcgat
     6001 ctgagccatg atgaaccgca ctcctctcat gcctcgcctt cgccgcctga tccacgcctt
     6061 cgccgcactc gcactgctgc tcgcggcggg caccggcatc gcgcgcgaca tggcagcgat
     6121 caagtccagc ggcacgctgg tggttgccgt gccggacatg cgcaatcccc cgttcttctt
     6181 cgaggacggc ggcgagctga aggggctcga catcgacctc gcaaaatcga tcgccgagtc
     6241 gcttggcgtg caggtcgcgt tcaaccgcga cgcaaagaac ttcaatgacg cggtccagct
     6301 cgtcgcgctc ggccgggccg acatggccgc ggccaagatc agccgcacgc tcgcgcgtgc
     6361 gcagacggtg ctgtttaccg acccctacct ggtgttgccg cacgcgctgc tgctcaaccg
     6421 cctgcgcttt gcggaaatct cacgcggccg agcgccggcc gacgtcctga agaacttcac
     6481 aggcagcgtg ggcgtcatcg ccaactcttc gttcgtcggt ttcgcgaaaa cccacttccc
     6541 ca
//
LOCUS       DATLGF010000286         3443 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_6020,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000286 DATLGF010000000
VERSION     DATLGF010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6020"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..819)
                     /locus_tag="VM406_08775"
     CDS             complement(1..819)
                     /locus_tag="VM406_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HVL76097.1"
                     /translation="MFGRTRPEIPEPLWQACLRDLPFLQGLGADDSARLKRLCEDLLD
                     KKSFTGAAGFELRDEVAVMIAAQACLPVLNLTLSLYDDMAGIIVYPAAFMIPQKEMDE
                     AGVMHEWHEPASGEAVDAGGAVVLSWEDAQEWDAPGYNVVIHEFVHKIDMADGSANGR
                     PPFLAPFHAGLDGRAWAQAFATAFEDFRQRVDEAEAQLPEDFNDDIAEHAALWDELCA
                     ALPLDPYAGTHPAEFFAVASEAFFVLPRPLHAAYPEVYRLLAAYFLQDPLASRP"
     gene            complement(834..1559)
                     /locus_tag="VM406_08780"
     CDS             complement(834..1559)
                     /locus_tag="VM406_08780"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HVL76098.1"
                     /translation="MPRFHCQIPLTVGAEIILPEAVAHHVQVLRLAPGDGITLFNGEG
                     GEYAAILTTVAKRQARALVQAFVPREAELPYALTLAQALPEGAKMDWIIEKAVELGVA
                     EVAPVAARRSVVRLSAERVAKKLEHWQGINASAAEQCGRNRLTRLREPVAVEDWLAGC
                     KVSSRILFSPRATQSLSDWVRGRAPQDLAILIGPEGGLTEEEEAAALRHKFVALSLGP
                     RVLRTETAGLAAVAAINAIWGQM"
     gene            1692..>3443
                     /gene="tkt"
                     /locus_tag="VM406_08785"
     CDS             1692..>3443
                     /gene="tkt"
                     /locus_tag="VM406_08785"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HVL76099.1"
                     /translation="MTSSLPTSKMANAIRALAMDAVQKANSGHPGMPMGMAEIAVALW
                     SRHYRHNPANPHWINRDRFVVSNGHGSMLHYALLHLTGYELSMDEIRNFRQLHSKTPG
                     HPEVHITPGIETTTGPLGQGITNAVGMALAETLLAYEFNRDGFPVVDHYTYVFLGDGC
                     LMEGISHEACSLAGTLGLNKLIALYDDNGISIDGKVEGWFTDDTPKRFEAYNWNVIRD
                     VNGHDVQAVDAAIAAAKKSDKPTLICCKTVIGKGAPNMQGSDKVHGAALGDKEVAATR
                     EAIGWPYAPFEIPDDVYAAWDAKAAGAAAEQEWNALFKAYDERFPQEAGELLRRMKGE
                     LPGALDATVEAFIASCVEKKETIATRKASQNAIQALAPVLPEFLGGSADLTGSNLTNW
                     KESIAVRLHQPGNHINYGVREFGMAAMMNGIALHGGFIPFGATFLTFSDYSRNAIRMA
                     ALMKTRTLFVFTHDSIGLGEDGPTHQSVEHVSSLRLIPNLDNWRPCDTVETAVAWAFA
                     VKRNDGPTSLIFSRQNLAFMERNSEQIGNIARGGYVLRDAADAKVVLIATGSEVELAM
                     KAADALAAEGIAVRVVSM"
BASE COUNT          636 a         1125 c         1118 g          564 t
ORIGIN      
        1 tcatggccgg gaagcaagcg ggtcctggag gaaatacgcg gcaagcaggc gatagacctc
       61 cgggtaggcg gcgtgcaagg gtctcggcag gacgaaaaat gcctcggaag ccaccgcgaa
      121 aaattccgcg ggatgcgtcc ccgcataggg atcgagcggc aaggccgcgc acagttcgtc
      181 ccacagcgcc gcgtgttcgg cgatgtcgtc gttgaaatct tcgggcagtt gcgcttcggc
      241 ttcatccacg cgttgccgga aatcctcgaa agccgtggca aacgcctggg cccaggcgcg
      301 cccgtcgagg ccggcgtgga agggcgcgag gaagggcggg cggccgttgg cgctgccgtc
      361 ggccatgtcg atcttgtgta cgaattcgtg gatcacgacg ttgtagccgg gtgcatccca
      421 ctcctgtgca tcttcccacg acagcaccac ggcgccgccg gcatccaccg cctcacccga
      481 ggccggctca tgccattcat gcattacacc ggcctcgtcc atttcctttt gcggaatcat
      541 gaaggcggcc gggtagacga tgatgccggc catatcgtca tacagggaca gtgtcaggtt
      601 caggaccggc aggcaggcct gggccgcgat catcaccgcg acctcgtcgc gcagctcgaa
      661 gccggcggcg ccggtaaagg attttttgtc cagcaggtcc tcgcacaggc gcttgaggcg
      721 cgcggagtcg tcggcgccga gtccttgcag gaaaggcagg tcgcgcaggc aggcttgcca
      781 gagcggctcc ggaatctccg ggcgcgttcg cccgaacatg cgccgcagcc agttcacatc
      841 tgcccccaga tcgcattgat cgcggccacg gcggccagcc cggcggtctc ggtgcgcagc
      901 acgcgcggcc ccagcgaaag ggcgacaaac ttgtgccgca atgccgccgc ttcttcttcc
      961 tccgtcaatc cgccttccgg gccgatcagg atggccaggt cctgcggcgc acggccacgc
     1021 acccagtcgg acagcgactg cgttgcgcgc ggcgaaaaca ggatgcggga ggagaccttg
     1081 catccggcca gccagtcttc aaccgcaacc ggctcgcgca gccgcgtcag ccggttgcgt
     1141 ccgcactgct cggcggcgga tgcgttgatg ccttgccaat gttcgagttt cttcgcaacc
     1201 cgctcggcgg acaggcgcac gacggagcgc cgggcggcga ccggcgcgac ctctgcgaca
     1261 cccagctcca ccgccttttc gatgatccag tccatcttcg cgccctccgg cagcgcctgc
     1321 gccagcgtca gcgcatacgg gagttccgct tcgcgcggca cgaacgcctg cacaagcgcg
     1381 cgggcttggc gtttcgcaac cgtggtcagg attgccgcat actcgccgcc ttcgccgttg
     1441 aacaaggtga tgccgtcgcc gggagcaaga cgcagcacct gcacgtggtg ggcgaccgcc
     1501 tcgggcagga tgatttcggc gccgacggtg aggggaatct ggcaatggaa gcggggcatg
     1561 aggcgaggcg gcaacgatgg tgtcccgatt ctaatgcaaa gggttcacat gcccgcctct
     1621 ggtaaaatac cgggctttgc acccgccggc tccggccggc tttcccgccc cgccttagcg
     1681 ttttttcaac gatgacttcc tctctcccga ccagcaagat ggccaacgcg atccgcgcct
     1741 tggcgatgga cgccgtacaa aaagccaatt ccggtcaccc gggcatgccg atgggcatgg
     1801 cagaaatcgc cgtcgcactg tggtccaggc actatcgcca caatccggcc aatccgcact
     1861 ggatcaatcg cgaccgcttc gtggtgtcca acggccacgg ctcgatgctg cactatgcgc
     1921 tgctgcacct gaccggctat gagctgtcga tggacgagat caggaacttc cgccagctgc
     1981 attcgaagac gccgggccac ccggaagtcc acatcacccc cggcatcgaa accaccaccg
     2041 gcccgctcgg ccagggcatc acgaacgcgg tcggcatggc gctggccgaa acgctgcttg
     2101 cctatgaatt caatcgcgac ggcttcccgg tcgtcgacca ctacacctat gtcttcctcg
     2161 gcgacggctg cctgatggaa ggcatctcgc atgaagcctg ttcgctggcc ggcacgctcg
     2221 gcctgaacaa gctgattgcg ctgtacgacg acaatggcat ctcgatcgac ggcaaggtgg
     2281 agggctggtt caccgacgac accccgaagc gcttcgaagc ctacaactgg aacgtgatcc
     2341 gcgacgtcaa cggccacgat gtgcaagccg tcgacgcggc gattgcggcg gcaaaaaaat
     2401 cggacaagcc caccttgatt tgctgcaaga ccgtgatcgg caaaggcgcg cctaatatgc
     2461 agggcagcga caaggtgcac ggcgcggccc tcggcgacaa ggaagtcgcg gcgacgcgcg
     2521 aagcgatcgg ctggccttac gcgccgttcg agattcccga cgatgtctac gcggcatggg
     2581 atgcgaaggc ggccggcgcg gcggcggaac aggagtggaa tgcgttgttc aaggcctacg
     2641 acgagcgttt cccgcaggag gcaggcgagc tgctgcggcg catgaagggc gagctgccgg
     2701 gcgcgctgga cgcgaccgtc gaagcgttca tcgccagctg cgtcgagaag aaggaaacga
     2761 tcgcgacccg caaggccagc cagaatgcga tccaggcgct ggcgccggtg ctgccggagt
     2821 tcctcggcgg ctcggccgac ctgaccggct ccaacctgac caactggaag gaatcgatcg
     2881 cggtgcgcct gcatcagccg ggcaaccaca tcaactacgg cgtgcgcgaa ttcggcatgg
     2941 ccgcgatgat gaacggcatt gccctgcacg gcggcttcat tccgttcggc gcgaccttcc
     3001 tgaccttctc ggactacagc cgcaatgcga tccggatggc ggcgctgatg aagacgcgca
     3061 ccctgttcgt gttcacgcac gattcgatcg gcctgggcga agacggtccg acccaccagt
     3121 cggtcgagca tgtgtcgagc ctgcgcctga ttccgaatct ggacaactgg cgcccctgcg
     3181 atacagtgga aacagcggtc gcatgggcat tcgccgtcaa gcgcaatgac ggcccgacct
     3241 cgctgatctt ctcgcgccag aatctcgcgt tcatggagcg caacagcgag cagatcggca
     3301 acatcgcgcg cggcggctac gtgctgcgcg atgcggccga cgcgaaggtg gtgctgattg
     3361 cgaccggctc cgaggtcgag ctggcgatga aggcggccga tgcgctggcg gccgagggca
     3421 ttgcggtgcg cgtggtatcg atg
//
LOCUS       DATLGF010000287         3732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_59150,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000287 DATLGF010000000
VERSION     DATLGF010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59150"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1525)
                     /locus_tag="VM406_08790"
     CDS             complement(125..1525)
                     /locus_tag="VM406_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HVL76100.1"
                     /translation="MIALTARMTRMHLGGGRLLLPASLVVAALVGIPVFGVLSNLLAI
                     DAGSETFSHLWATVMPEYLFNSFAIAAIVAVLAGAGGVGCAWFVAAYEFPGKRWFEWA
                     LVLPLAMPAYVVAYAYTDFLQFAGPVQSALREAFGWRAGDYWFPEIRSIGGAGVLFAM
                     VLYPYVYLLARNAFLERSPRMADAARTLGMGPWRTFFTVSLPLARPAAVAGIALALME
                     TLADYGAVAYFGVPTLTTGIFKSWYAFSDRTAAAQIAAVLLLTVVILMVLEQKSRGRA
                     RYYAVGSRLKQDVPHRLRGWRGWAATVFCAVPVVLGFLLPIVILVRLMVREEHIILSG
                     RYLHWLYNSVFLGAVTALIAVAVCLLLGYAARATGSKVQAACNRIVSMGYAIPGAVIA
                     VGILIPLSRLDNWAADQGWKLALTGSVVALIYAYLVRFLAVSYQSVQAGLTKITPSMD
                     ASTRRSCGAACSRPAC"
     gene            1613..2707
                     /locus_tag="VM406_08795"
     CDS             1613..2707
                     /locus_tag="VM406_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL76101.1"
                     /translation="MKATPDFPHLSVDAIRVGYPVGRRVQEIVHGLSFALARGEIGCL
                     LGPSGCGKTTVLRAIAGFESLIDGRIALGGRELSSAGHTAAPESRQVGVVFQDYALFP
                     HLTVWDNVAFGLRKLPLAQRKSRVDRLLAMVGLVAHGRKYPHELSGGQQQRVALARAL
                     APQPDLLLLDEPFSNLDIDLRERLALEVRDILRELGTTAVLVTHDQHEAFAIADQIGV
                     MREGRIVQWDTAYNLYHRPANRFVADFIGQGVFARGTVDLPQQKVHIELGSLPLWQGV
                     DVCATDQRDAGEVDVLLRADDVIHDDASPLRAEVVRKAFRGAEFLYTLRLPSGQELLA
                     LVPSHHDHEIGEQIGIRLGADHVVTFPAEQ"
     gene            complement(2685..3524)
                     /locus_tag="VM406_08800"
     CDS             complement(2685..3524)
                     /locus_tag="VM406_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HVL76102.1"
                     /translation="MRPILIAFGRAVLSQLHLRMLLLTILPFLLSIAIWGVLLWLFLE
                     PMVDWLHAWFSETGHFATAGGVLEWMGLGALKTLVVPLIAMWVLLPLMVLTALLCVGV
                     FAMPVIVGHVSRRHFPELEKRRGGSFLGSIWVGASSFLLFALLWVLTLPLTLIPPLTF
                     IIQPLLWGWLTYRVIAYDALAEHASKEEHAWLMRVHRWPLLAIGAIAGAMGAAPAMLW
                     LGGALLILFPVLAAGAIWLYVLVFVFSGLWFQHYCLQALATYRALYERDTQTLPAIAQ
                     PER"
BASE COUNT          731 a         1247 c         1222 g          532 t
ORIGIN      
        1 ggcagtgccg cttcgccgag ccgctcgtcg ctggcgagct gatgggtaat gacggccagc
       61 gtgtcgaaat tgaacggccg tatcgtcagc gtcgccggca gttcctttac cacgtcgacg
      121 aaaactagca ggccggccgt gagcaggctg cgccacagga gcggcgcgtg ctggcgtcca
      181 tgctcggcgt gatcttcgtc aggccggcct gcacgctctg gtaggacacc gcgagaaaac
      241 gcaccaggta tgcatagatc agcgccacca cgctgccggt cagcgccagc ttccaaccct
      301 ggtctgccgc ccagttgtcc aggcgcgaca gcggaatcag gatgccgacc gcgatcacgg
      361 ctcccgggat cgcgtacccc atgctgacga tgcggttgca ggcggcctgc accttgctgc
      421 cggttgcgcg cgcggcgtaa ccgagcaaca ggcagaccgc gaccgcgatc agcgccgtca
      481 ccgcgccgag aaagacgctg ttgtacagcc agtgcagata gcggccggac aggatgatgt
      541 gctcctcgcg caccatcagc cgcacaagga tcacgatcgg cagcaggaag ccgagcacga
      601 ccggcactgc gcagaacacc gtcgcggccc agccgcgcca gccgcgcagc cgatgcggca
      661 catcctgctt gagccggctg ccgaccgcgt agtagcgcgc ccgtccgcga ctcttctgct
      721 cgagcaccat caggatcacg acggtcagca gcagcacggc ggcgatctgc gcggcggcgg
      781 tgcggtcgga gaatgcatac caggacttga agatgccggt ggtcagcgtc ggcacgccga
      841 agtaggcgac cgcaccgtag tcggccaacg tctccatcag ggccagcgca atcccggcga
      901 ccgccgccgg gcgggccagc ggaaggctca ccgtgaagaa ggtgcgccag gggcccattc
      961 caagcgtgcg cgcggcgtcg gccatgcgcg gactgcgctc cagaaaggcg ttgcgcgcaa
     1021 gcagatagac gtatggatag agcaccatcg cgaacagcac gccggcgccg ccgatggaac
     1081 ggatctcggg aaaccagtaa tcgccggcgc gccagccgaa ggcttcgcgc agcgcagact
     1141 ggaccgggcc ggcaaactgc agaaagtcgg tgtacgcgta ggccaccacg taagcgggca
     1201 tggccagcgg cagcaccagc gcccattcga accagcgctt gccgggaaat tcataggcgg
     1261 cgacgaacca ggcgcagccg accccgccgg cgcccgccag cacggcgacg atggccgcga
     1321 ttgcaaagct gttgaacagg tattccggca tgaccgtcgc ccacaggtgg gagaaggtct
     1381 cgctgccggc atcgatggcg aggagattgg agaggactcc gaataccgga atgccgacca
     1441 gggctgctac caccagcgaa gccggcagca acaggcggcc gccgcctagg tgcatgcgcg
     1501 tcatgcgcgc ggtcaaggca atcatcgaac tccacttttc cggtacggtg tctctgaatg
     1561 agaattataa tcaatatcga ttttcgctct cgcaatcttt acgccccttc cgatgaaagc
     1621 aactccggac ttccctcatc tgagcgtcga cgcgatccgc gtcggttatc cggtcggccg
     1681 ccgcgtgcag gaaatcgtgc acgggttgag tttcgcgctc gcgcgcggcg agatcggctg
     1741 cctgctgggg ccgtccgggt gcggcaagac cacggtcctg cgggcgattg ccggcttcga
     1801 atccctgata gacgggcgca tcgcgctcgg cgggcgcgag ctgagcagcg ccggccatac
     1861 cgcggcgccg gagtcgcggc aggtcggcgt ggtgttccag gactacgcgc tgtttccgca
     1921 tcttacggtc tgggacaacg tcgcattcgg gctgcgcaag ctcccgctcg cgcagcgcaa
     1981 gagccgggtc gaccgtctgc tggcgatggt cgggctggtc gcgcacgggc gcaagtatcc
     2041 gcacgagttg tcgggcggcc agcaacagcg cgtcgcgctg gcccgcgccc tcgctccgca
     2101 gccggatctg ctgctgctgg acgaaccgtt ctcgaacctc gacatcgacc tgcgcgaacg
     2161 cttggcgctg gaagtgcgcg acatcctgcg cgaactgggc acgacggcgg tgctggtgac
     2221 ccacgaccag cacgaggcct tcgcgatcgc cgaccaaatc ggcgtcatgc gcgaaggccg
     2281 gatcgtgcag tgggacacgg cgtacaacct gtaccaccgc ccggcgaacc gtttcgtcgc
     2341 ggacttcatc ggccagggcg tgtttgcccg tggcaccgtc gacctgccgc agcagaaggt
     2401 gcatatcgaa ctggggagcc tgccgttatg gcaaggggtg gacgtctgcg ccaccgatca
     2461 gcgcgacgca ggcgaggtcg acgtgctgct gcgcgccgac gacgtcatcc atgacgacgc
     2521 cagcccactg cgcgccgagg tggtgcgcaa ggccttccgc ggcgccgaat tcctgtacac
     2581 gctgcgcctg ccgagcggac aggaactgct ggcgctcgtg ccctcgcacc atgaccatga
     2641 aatcggcgag cagatcggca tccgcctggg cgccgaccac gtcgtcacct ttccggctga
     2701 gcaatagctg gcaacgtctg tgtatcgcgc tcatagagcg cgcggtaggt ggccagcgcc
     2761 tgcaagcaat agtgctggaa ccacaggccg gagaacacga acaccaggac gtacagccag
     2821 atcgcgcctg cagccagcac aggaaacagg atcagcaagg cgccgcccag ccacagcatc
     2881 gccggcgccg cccccatcgc gccggcaatc gcgccgatgg ccagcaaggg ccagcggtgc
     2941 acgcgcatga gccacgcatg ctcttccttg ctggcatgct cggccagcgc gtcgtaggca
     3001 atcacacgat aggtcagcca gccccacagc aagggctgga tgatgaaggt cagcggcggg
     3061 atcagcgtca acggcaaggt cagcacccat agcagcgcga acagcaggaa ggacgaggcg
     3121 ccgacccaga tgctgcccag gaaacttccg ccccggcgct tctccagctc cggaaaatgg
     3181 cggcgcgata catggccgac gatgaccggc atcgcgaaca cgcccacgca cagtagcgcc
     3241 gtcaacacca tcagtggcag caggacccac attgcgatca gcggcacgac cagcgtcttg
     3301 agcgctccaa gccccatcca ttccagtacg ccgcccgccg tcgcaaaatg cccggtctcc
     3361 gagaaccagg cgtgcagcca gtcgaccatc ggctccagga acagccacag gagcacgccc
     3421 cagatcgcga tcgacagcag gaacggcagg atggtcagca gcagcatgcg caggtgcagc
     3481 tggctgagca cggcgcggcc gaatgcgatc aggatgggac gcatggtttt ccttgttgta
     3541 cgcacaagat tgacaggttg gaactgggca cgaattctgc tcgcgggaat gatgaaggcg
     3601 aaggggccga cgatagcacc ggcccggggc cggcacaacg agacatgttc ctgatccagc
     3661 tgctgcttcc gctctacgac aacgcgggca cccccatcga cggggcgtgc tacgcgcaga
     3721 cgcgcacgga gc
//
LOCUS       DATLGF010000288         2331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_118837, whole genome shotgun sequence.
ACCESSION   DATLGF010000288 DATLGF010000000
VERSION     DATLGF010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118837"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VM406_08805"
     CDS             complement(<1..806)
                     /locus_tag="VM406_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilY"
                     /protein_id="HVL76103.1"
                     /translation="MKTRILACVLLAAAVAEAASAPPAVDIAREPLYNGRANVHPNLL
                     LSLSVEFPTVGIAYRGDNGTYNRTKEYIGYFNPAKCYVYEGGNRNLSDAAYFRIHKDA
                     DPVTRECGGDSFSGNFMNWAASSAIDMLRYALTGGDRVIDTPELTVLQRAVLRDAASS
                     NFYAHPTYFPRRAVKGGGNVSAPGQVTPFNVDTLYVVSCRNRILFSDIDSGVSGNSAY
                     CTAAYDGKGKPSDEARDKKLGEYLARVRVCDAAEGPMRSDLCQRYGKHYKP"
     gene            complement(803..1390)
                     /locus_tag="VM406_08810"
     CDS             complement(803..1390)
                     /locus_tag="VM406_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HVL76104.1"
                     /translation="MRHSFRSRRELGAALIVALMMLVGVLALAVSAAQIAIQAERAAR
                     NERDRHVAFQAAEAALLDAEMDIEDGGSRSGLFAHDSIDGFEAGCGTAGDARGLCLRA
                     AAGTPVWLAMDFLDPDSAAVDFGSFTGKSFPGGSGPLPARPPRYLIEVMPFNRAGEGA
                     TVEDITYFYRVTAIGFGARDSTQVVLQTFYRKHAQ"
     gene            complement(1387..>2331)
                     /locus_tag="VM406_08815"
     CDS             complement(1387..>2331)
                     /locus_tag="VM406_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HVL76105.1"
                     /translation="VLVTTGLLLSTRSSFLTQDDDIHLHDSARFAFELIARSVRQAAY
                     ENWDTDEAPVLAADDLTPNIRGLNARRLSASTAGGVDPLAASGSPVNGSDVLAVRFFG
                     SGPPATPGAWTGGDGTVLNCAGASVPTVQSLDEAEERRGWSIFYVARSASNANEPELY
                     CKYRGPGAYAATAIVPGVESFQVLYGVDIDGDGMPERYLHAGQVDALDDALVLTGASP
                     AEQAADLRRRTHWKRIATVRIALLVRGANPARADLPGTRYDLFGADYSLAAGGADPGV
                     RIEESAIAAGERNRMRRVFSTTIQLRNRSAGSGAGAGT"
BASE COUNT          382 a          761 c          795 g          393 t
ORIGIN      
        1 ggcttgtagt gcttgccgta cctctggcac aagtcgctgc gcatcggccc ctccgcggca
       61 tcgcacacgc gcacgcgcgc caggtattcg cccagcttct tgtcgcgcgc ctcatccgag
      121 ggcttgccct tgccgtcata ggctgcggtg cagtaggcgc tgttaccgga gacgccgctg
      181 tcgatgtcgc tgaacaggat gcggttgcgg catgacacca cgtagagcgt atcgacattg
      241 aacggcgtga cctgccccgg agcgctgacg ttcccgccgc ccttgaccgc gcgccgggga
      301 aaataagtcg ggtgcgcata gaagttgctg ctggcggcgt cgcgcagcac cgcgcgctgc
      361 aagacggtga gttccggcgt gtcgatcacg cgatcgccgc cggtcaaagc gtagcgcagc
      421 atgtcgattg ccgacgaggc ggcccagttc atgaagttgc cgctgaatga atcaccgccg
      481 cattcacgcg tgaccggatc ggcgtccttg tgaatgcgga aataggccgc gtcgctcagg
      541 ttgcggttgc cgccttcgta gacatagcac ttggccggat tgaagtagcc gatgtattcc
      601 ttggtgcggt tgtacgtgcc attgtcgccg cggtacgcga tgccgaccgt cggaaattcg
      661 accgacaggc tgagcagcag attgggatgc acattggcgc gcccgttgta gagcggctcg
      721 cgggctatgt cgaccgcggg cggggcgctg gcggcctcgg cgacggcggc cgcgagcaat
      781 acgcaggcga ggatacgcgt cttcattgcg catgcttcct gtagaaggtc tggagtacga
      841 cctgggtgga gtcacgggca ccgaagccga ttgccgtgac gcggtagaag taggtgatgt
      901 cctccacggt cgcgccttct ccggcgcgat tgaacggcat gacttcaatc aggtagcgtg
      961 gcgggcgtgc aggcagcggt ccggacccgc cgggaaagct cttgccggtg aagctgccga
     1021 aatcgacagc cgcggaatcg gggtcgagaa aatccatcgc gagccagacc ggcgtgccgg
     1081 ccgccgcgcg caggcacagg ccgcgggcgt cgccggccgt gccgcaaccg gcctcaaagc
     1141 cgtcaatgct gtcatgcgcg aacagcccgc tgcgcgatcc tccatcctca atatccattt
     1201 cggcgtccag cagcgccgcc tcggcggcct ggaaagcgac atgccggtcg cgctcgttgc
     1261 gcgccgcgcg ttcagcctgg atcgcgatct gtgccgccga tacggcgagc gccagcacgc
     1321 ccaccagcat catcagcgcc acgatcaacg cggcaccaag ctcgcgtcgc gacctgaacg
     1381 agtgcctcat gttccggctc ccgcgccgct gccggcgctg cggttgcgca gctgtatggt
     1441 ggtcgagaat acgcgcctca tccggttgcg ttcgccagcc gcgatggcgg attcctcgat
     1501 gcgcacgccc gggtcagctc cgccggccgc gagcgaatag tcggcaccga acaggtcgta
     1561 acgcgtgccc gggaggtctg cgcgtgcagg attggcgccg cgcaccagca gggcgatacg
     1621 cacggtggcg atgcgcttcc aatgggtgcg cctgcgcagg tcggcggcct gctcggcagg
     1681 actggctccc gtcagcacga gcgcatcgtc cagagcatcg acctgcccgg cgtgcagata
     1741 gcgctcgggc atgccgtcgc cgtcgatgtc gacaccgtaa aggacctgga acgattcgac
     1801 gccgggaacg atggcggtgg cggcgtaggc gccaggtccc cgatatttgc agtacagctc
     1861 gggttcgttc gcattgctgg ccgaacgggc gacgtagaag atgctccagc cgcgccgctc
     1921 ctcggcctcg tccagcgatt ggactgtcgg gacgctggcc ccggcgcaat tgagcaccgt
     1981 gccgtcgccg ccggtccacg cacccggcgt ggccggcggg ccggatccga agaagcggac
     2041 ggcgagtaca tcgctgccgt tgaccgggct gccgcttgcc gccagcggat cgacgccacc
     2101 cgccgtactg gccgagagcc ggcgcgcatt caagccgcgg atgttcggcg tgaggtcatc
     2161 ggcggccagc accggcgcct cgtccgtgtc ccagttttcg tacgcggcct ggcgcaccga
     2221 gcgggcgatc agctcgaacg caaagcgtgc actgtcatgc agatggatgt cgtcgtcttg
     2281 ggtcaggaag ctggacctgg tcgataacag gagaccggtc gtcaccagca c
//
LOCUS       DATLGF010000289         5238 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_138671, whole genome shotgun sequence.
ACCESSION   DATLGF010000289 DATLGF010000000
VERSION     DATLGF010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138671"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1595
                     /locus_tag="VM406_08820"
     CDS             <1..1595
                     /locus_tag="VM406_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HVL76106.1"
                     /translation="VPFAKAPVGELRWRAPADAEPWNGTRAATAFGPACSQYGRIYGP
                     GANNRYDLTIGTTLNQAVGSEDCLYLNIWRPANARPDLPVIVFFHGGSNVSGYTADPM
                     YDGANLAKTTNSVVVTANFRLGILGFLNLAQMKDGEDADTASGNFAILDSIKALEFLQ
                     QNIANFGGDPGNVTIMGESAGAINVYALLTSPKLADAQRKLFHRAVPLSGGLSLAENL
                     PAGTIPTMSPAAVYALQGNALLVNLLVADGSAADLAAAQTIAAGMSPAQVGAYLRGKQ
                     PSELFATLLTRLAAQGLAGSGPIPDGRIVAHNPVNAIGQGRYVRAPVLAGNTRDEGKL
                     FPGFLGLVSGIPGSARIVSDAQLFAHHYHYRPNDAPQTTVQEWIAPGYLPVNQPVTGF
                     TARAEQFNRIFFLASRDNILNTLKAVQPDVWYYRFDWDEQPAPWNDIYGAAHLFDLPF
                     VFGNFGPSLMANIMNTEANRQGRLQLSDAMMRSIGAFAQRGDPNNGALGVQWPTWPMG
                     LIFDATKTKKVIGVEPIQPALP"
     gene            complement(1665..2441)
                     /locus_tag="VM406_08825"
     CDS             complement(1665..2441)
                     /locus_tag="VM406_08825"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HVL76107.1"
                     /translation="MDAPQTFNPLTPLYHDPWLLAVHKPAGLLVHRSPIDPHETEFAL
                     QYARAMNGGRHVYPVHRLDRPTSGLLLFALDPLTARVLGKAMMAGEVRKTYLAMVRGW
                     TPEQGLIDHPLRDEPEDRRQKGVAQVEREARTAYRRLATTEIPVQIEGYPTSRYSLVV
                     LYPETGRRHQLRRHMKHISHPIIGDAYHGRGRHNRYFAERFGQGRLMLAATGMTLRHP
                     VTHLPLELIAQPEASFMRVLSIFGEGPGGIGELFDRSALE"
     gene            2926..3096
                     /locus_tag="VM406_08830"
     CDS             2926..3096
                     /locus_tag="VM406_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76108.1"
                     /translation="MIVTRAVYEAVRQIHQQRVQYDPASPVHKTALDAGVAQVNGEMI
                     ELTFKGELVLDL"
     gene            complement(3227..4138)
                     /locus_tag="VM406_08835"
     CDS             complement(3227..4138)
                     /locus_tag="VM406_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVL76109.1"
                     /translation="MLNLNDVYFFVQAVESQGFAPASRRLGIPKSTLSKRVAALEAEL
                     GVRLIQRSSRRFVLTELGQDFHRHAAAMLIEAEAAEHVVKGRLAEPSGVVRITSSVPT
                     AQLMLADVVVEAARRYPKLRIVVHASDRFVDIVQEGFDIALRDHFNPLPDSGLVQRRI
                     ATDPLYLVASPAYLQQRDTPREPADLAAHDALATAPGAAAWKLENEAGERIEVQAPAR
                     FTADESTVLLRAAAAGLGIVSLPRKLCQPSLDAGELVRVLPGWIAGGVTTTLLMPHRR
                     GQLPSVRAVVELLVQPGGSQLQVGMAM"
     gene            4281..5057
                     /locus_tag="VM406_08840"
     CDS             4281..5057
                     /locus_tag="VM406_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010094497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVL76110.1"
                     /translation="MFIPGCLFLYKENCMEPVLFYGVPQGCSFGSIVALEWLGEPYRL
                     CRIEMMERPWPAAYAKLNPRMLTPALLTDDGAPLVESLAILQHIGSRKLGAGIGFAQG
                     TREFDRLNQMLSYLMTNFFFSFGPLWTVYETEGLTDAQKDLLRRMGEEDVRNQCAYVD
                     RVLAGSEWLLGNRKTVADAYMAGVARWAEYHRLFDMEAEYPHFARYLKTLRSDPAVRF
                     ADAIEKGERARGNGSFKGHVALDEVLDLAAAKRTPERVVS"
BASE COUNT          896 a         1744 c         1714 g          884 t
ORIGIN      
        1 gcgtgccgtt tgcaaaagcg ccggtcggcg agctgcgctg gcgcgcgccg gccgacgccg
       61 aaccctggaa cggcacccgc gccgccaccg cgttcggccc ggcctgctcg cagtacggcc
      121 gcatctacgg ccccggtgcg aacaaccgct acgacctcac gatcggcacc acgctcaacc
      181 aggcggtggg cagcgaagac tgcctgtacc tgaacatttg gcgcccggcc aacgccaggc
      241 ccgacctgcc ggtgatcgtg ttcttccatg gcggcagcaa cgtctccggc tatacggcgg
      301 accccatgta tgacggcgcc aatctcgcca agaccaccaa ctccgttgtc gtcaccgcca
      361 acttccggct cggcattctc ggcttcctga acctcgcgca gatgaaggac ggtgaagacg
      421 ccgacaccgc cagcggcaac ttcgcgatcc tcgacagcat caaggcactg gaattcctgc
      481 agcagaacat cgcgaacttc ggcggcgacc cgggcaacgt gacgatcatg ggcgagtccg
      541 cgggcgcgat caacgtgtat gcgctgctca cctcgccgaa gctggccgac gcccagcgca
      601 agctgttcca ccgcgcggtg ccactctccg gcggcctgtc gctcgcggag aatctcccgg
      661 ccggcaccat cccgacgatg tcgccggccg cggtctacgc cttgcaaggc aacgcgctgc
      721 tggtgaacct gctggtggcc gacggcagcg ccgccgacct cgccgccgcg cagacgattg
      781 ccgccggcat gagccccgcg caggtcggcg cctacctgcg cggcaagcag ccgtccgaac
      841 tgttcgcgac gctgctgacc cggctcgccg cccaaggcct ggccggttcc ggtccgatcc
      901 ccgacggccg catcgtcgcg cataacccgg tcaacgcgat cggccagggc cgctacgtgc
      961 gcgcgcctgt cctcgccggc aacacccgcg acgaaggcaa gctcttcccc ggcttcctcg
     1021 gactggtctc gggcatcccc ggcagcgcac gcatcgtcag cgacgcccaa ctgttcgccc
     1081 accactacca ctaccggccg aacgacgcgc cgcagacgac ggtgcaggaa tggatcgccc
     1141 ccggctacct gccggtgaac cagcccgtca ccggcttcac cgcgcgcgcc gagcagttca
     1201 accgcatctt cttcctcgcc agccgcgaca acatcctcaa cacgctcaag gccgtgcagc
     1261 cggacgtctg gtactaccgc ttcgactggg acgaacagcc ggccccgtgg aacgacatct
     1321 acggcgccgc ccacctgttc gacctgccct tcgtgttcgg caacttcggt ccttcgctga
     1381 tggccaacat catgaacacc gaagcgaacc gccagggccg gctgcagctg tcggacgcga
     1441 tgatgcgcag catcggcgca ttcgcacagc gcggcgaccc gaacaacggc gcgctcggcg
     1501 tgcaatggcc gacctggccg atggggctga tcttcgatgc gacaaagacg aagaaggtga
     1561 tcggggtgga gccgattcag ccggcgttgc cgtagggtgc aggcaggcgg caaatgccgc
     1621 tcacgacctc aatgctgtcg cccccgcgca ggcgggggcc cagctcactc taatgcggag
     1681 cgatcgaaca gctccccaat gccgcccggg ccctccccga aaattgagag cacccgcatg
     1741 aaactcgcct ccggctgcgc aatcagttcc agcggcagat gcgtgaccgg atggcgcagc
     1801 gtcatgccgg tcgctgcaag catcagtcgc ccctgcccga accgttcggc gaaataacgg
     1861 ttgtggcggc cgcgcccatg gtaggcatcg ccgatgatcg gatggctgat gtgcttcatg
     1921 tgccgccgca actggtgcct gcgaccggtt tccggataca gcaccaccag gctgtaccgg
     1981 ctggtcggat agccttcgat ctgcaccgga atctccgtcg tcgccaggcg gcggtatgcg
     2041 gtgcgcgcct cgcgctcgac ctgcgccacg cccttctgcc gccgatcctc cggctcgtcg
     2101 cgcagcggat gatcgatcag cccttgctcc ggcgtccagc cgcgcaccat cgccagataa
     2161 gtcttccgca cctctcccgc catcatcgcc ttgccgagta cgcgggcggt gagcggatcc
     2221 agcgcgaaca gcagcagccc cgacgtgggg cgatccagcc gatgcaccgg gtagacatgc
     2281 cggccgccgt tcattgcgcg cgcgtactgc agcgcgaact cggtttcgtg cggatcgatg
     2341 ggactgcggt gcaccagcag gccggcaggc ttgtgtacgg ccagcagcca ggggtcgtgg
     2401 tagagcgggg tgaggggatt gaaggtttgc ggggcgtcca tgtctggtcc cggatgctgc
     2461 acaagatcct gaagcggtgt tcgtcgccca gcataacttc aatttacttg ttgatgctgg
     2521 tatcgctgct cccgccacga gcgggcagcg tcgtcttcgc tttccctgtc gcgatgatcg
     2581 aggcggctct gcaggaggcc cggccggtgc tttccgacgc gatgaggcgc agcatcggcg
     2641 cattcgcact gcgcgccgac ccgaactcgg cgagcgggac gtgcaatggc gggactggcc
     2701 gatggggccg accgtcgatg caacgcggac gaagaaggtg agtcgcgtgg aggcgctacc
     2761 gccggcaccg cggtagcggc gcagcgacgg cgcaccgggg cgggagcgag gatggcgccg
     2821 ccgcgtccgg caagacggca tcgaaatcgc tcaatttgcg cgatcgccgg cttatcccgc
     2881 gtcaattccc ggtaagatgc ggtttttctc caaccaggac atcccgtgat cgtcacccgc
     2941 gccgtctacg aagccgttcg tcaaatccat cagcagcggg tccagtacga ccccgccagt
     3001 ccggtccaca agaccgcgct ggacgccggg gttgcccagg ttaacggcga aatgatcgaa
     3061 ctcacgttca agggagaact ggtgctcgac ctttgagcat cgatgcaggc gcggcgtgcg
     3121 tacaacgcta cgcgccgtgc acggaacgac cgccattgcg ccccggccgc gtgccactcc
     3181 ggcaattcgc caggctgccc gcaccccggc ccgcgacggc gaggcattac atggccatcc
     3241 ctacctgaag ttggctgcct cccggctgca ccagcaactc caccaccgcc cgcaccgaag
     3301 gcaactgccc ccgccgatgc ggcatcagca gcgtcgtcgt caccccgcct gcaatccacc
     3361 ccggcagcac gcgcaccagc tctcccgcat ccaggctcgg ctggcacagc ttgcgcggca
     3421 ggctgacgat gcctagtcca gctgcggccg cgcgcagcag cacggtggat tcgtcggcgg
     3481 taaagcgcgc aggcgcctgc acctcgatgc gttcgccggc ttcgttttcc agtttccagg
     3541 cggccgcgcc cggcgcggta gccagtgcgt cgtgcgcggc taggtcggcc ggttcgcgcg
     3601 gcgtatccct ctgctgcaga tacgcgggtg aggcgaccag gtagagcggg tcggtggcga
     3661 tgcgccgttg caccaggccg gagtcgggca aggggttgaa gtggtcgcgc agggcgatgt
     3721 cgaagccttc ctgcacgatg tccacgaagc ggtcgctggc gtggacgacg atgcgcagct
     3781 tcgggtacct gcgcgcggct tcgaccacca cgtcggccag catgagctgg gccgtgggca
     3841 cggaggaggt gatgcgcacc accccgctcg gctcggcgag ccggcccttg accacgtgtt
     3901 ccgcggcttc ggcttcgatc agcatggcgg cggcgtggcg gtggaagtcc tgcccgagtt
     3961 cggtgagcac gaagcggcgc gaggagcgct ggatcaggcg cacgccgagc tcggcttcga
     4021 gcgcggccac gcgtttgctg agcgtggatt tcgggatgcc gagccggcgc gaggcggggg
     4081 cgaagccctg gctttcgacg gcctggacga agaagtagac gtcgttgagg ttcagcatgg
     4141 cttggtcgcc ccgggttgtt cgctttgatc gtccatagtt ggagacgcta agtctaaatt
     4201 tgcccgtcta ttcgatcaac gtccacgcta ctacagttct cccacagctg ctgattgcgg
     4261 ccgattgcag cttattcccg ttgttcattc ccggctgctt atttctttac aaggagaatt
     4321 gcatggaacc cgtgctgttt tatggcgtgc cacaggggtg ctcgtttggc tcgatcgtgg
     4381 cgctggagtg gctgggcgag ccgtatcggc tgtgccgcat cgaaatgatg gagcggccgt
     4441 ggccggcggc ctacgcgaag ctcaacccgc gcatgctgac gcccgcgctg ctcaccgatg
     4501 atggcgcgcc gctggtcgag agcctggcga tcctgcagca cattggttca cgcaagctcg
     4561 gggcgggcat cggctttgcg cagggcacgc gcgagttcga tcggctcaac cagatgctct
     4621 cttacctgat gacgaatttt ttcttttcct tcggcccact ctggacggtg tacgaaaccg
     4681 aggggctgac ggatgcgcag aaagacttgc tgcgccggat gggcgaggag gatgtgcgca
     4741 atcagtgcgc gtatgtggac cgtgtgctcg ccggctcgga gtggctgctg gggaaccgca
     4801 aaacggtggc ggatgcctac atggcgggcg tggcgcgctg ggcggagtat caccggctgt
     4861 tcgacatgga agcggagtat ccgcactttg cgcgttacct gaagacgctg cggagcgatc
     4921 cggcggtgcg ctttgcggac gcgatcgaga agggggagcg cgcgcggggc aatgggagct
     4981 tcaaggggca cgtcgcattg gatgaggtac tcgacctggc tgcggctaag cgaacgccgg
     5041 agcgcgtggt cagctaaagg ggctcgttcg atggacgagg ttcgcaaggt cctgcatgag
     5101 cctgtcagat tttttgtgtg caagaggtca tgagatgata ccgaaaggga aacaccgtga
     5161 ccgatgtaat gagcaccaag aagacgaaga aaccgaaggc agcgaagcta tttccagatg
     5221 agttgattga ccagttgc
//
LOCUS       DATLGF010000290         6104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_59205,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000290 DATLGF010000000
VERSION     DATLGF010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59205"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VM406_08845"
     CDS             <1..891
                     /locus_tag="VM406_08845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HVL76111.1"
                     /translation="ALSLDHLDLADIAAGIVEASKEDPAARDRHITVETSEPLWVHAD
                     RIRIEQVILNLVNNAIKFTAPGGHIRVTLKRAGREAVLEVKDDGRGIDTDFLPHVFDM
                     FRQGRTGPNRSNSGLGIGLALARQIVELHNGVITAESGGNGRGACFCVRLPLSDAPRN
                     TGDDADQGAGQALHGVEVLLVDDNAEAVDSFKYLLEFEGAKAHVTRTAQDALACLDKQ
                     RVDLIISDLAMPGMDGFALMEQVRARYGKKSPLAIAVSGLGRAQDEERALQAGFAAHL
                     TKPVSIDMLMQTAAKLLGKG"
     gene            997..2004
                     /locus_tag="VM406_08850"
     CDS             997..2004
                     /locus_tag="VM406_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HVL76112.1"
                     /translation="MPIRSRIVVIGTSAGGPDALRRLLRRLPADFPAAILAVMHIGSH
                     YSTLPRILQRDASMPVRHAEDGAPIEPGTVLLAPPDRHLMVEDGTTRLSSGPKENFSR
                     PAIDPLFRTAALFYRENAIGLVLTGLLDDGTIGLQAIKAYGGIAMVQDPHEADEPAMP
                     KSALEHVRVDACLPLDELADRLLELVRSAPPPVEAGRQVKAIETQGWFDLRQNTDLTE
                     LGEIAVPSSFTCPECSGSLWEVRDSNPPYFRCHTGHAYTAQGLNSAQDMAIEEAVWAA
                     IRALHEKQMLLHRLAGQAASANRDAAVQEHVATAETVARHAEILRRVVARHDWPEDLT
                     G"
     gene            complement(2103..2825)
                     /locus_tag="VM406_08855"
     CDS             complement(2103..2825)
                     /locus_tag="VM406_08855"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HVL76113.1"
                     /translation="MQAVDLTWRHQNRLLELLPAPEFVHIAPLLSVHEVEVKHVLIRK
                     GAPLRHVDFPCSSVYSALVEMADGATVEVGTIGNEGMASLNALLDGHVATETLICQIP
                     GISLRMRIDDFQWEAESNAQFRTVLDLYAQAYISQVEQSAACNQLHTLEQRTARWLLM
                     THDRVRQDVFPMTQEFLSFMLGVQRPSVSQIARRLEQEGALSYHRGMMRILRRDLLEA
                     TTCECYAATRSQFEHLLGASAG"
     gene            complement(2890..3489)
                     /locus_tag="VM406_08860"
     CDS             complement(2890..3489)
                     /locus_tag="VM406_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76114.1"
                     /translation="MTSISLLLERRARGSAPVAGIVRRRSARFCSVADIRSVSKAALA
                     VLRGLSFLRIACTATLDPARASPDGCDRGTEPLNETRLTAHAETPGASASPDPAARPV
                     RGRTLMMTFPRPVDERAQLAAANRHIAMAERRIAEQRDRIEELRKAGHACRSSEDLLA
                     LLLDGLSAMHLHREQILRALADIRRDRRRGVKGATGPRG"
     gene            3479..4339
                     /locus_tag="VM406_08865"
     CDS             3479..4339
                     /locus_tag="VM406_08865"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HVL76115.1"
                     /translation="MDVIDTNLRQAVIRSDPAALSECLWRGAEVNARDANGWTAMHFA
                     CSCADARIALKLLAHGADVQVNDKGGVGPLHLAAMRRDASLVRLLLDAGAFPDARDRY
                     GATPLFLAAGRGNISAVTALLRAGARVDRSDGLGYNALHWAASNRNPRTARLLLRAGA
                     PLHAVENEGMTPLHIAVLGGHLHTARFLIQQGSIVDQQDAAGQTALHFAVRRCDAELA
                     ALLLSAGANMHEASAAAETPFDVACWQNDPVVLDVLYRTETANEDSFFVAGNAPQAAG
                     LEPWQGDPLA"
     gene            4460..>6104
                     /locus_tag="VM406_08870"
     CDS             4460..>6104
                     /locus_tag="VM406_08870"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HVL76116.1"
                     /translation="MLTETAHTGAVPGRVAFPVVGIGASAGGLPALVTLLENMPSSPG
                     MALVVILHLAPDQPSAVDRVLQRATDMAVAQAGQREFIMPDHVYVIPPDRSLRMEDGY
                     LYVEPLDHPAHGRLVTIDRFFRTLAEAQKERAIGIVLSGMGTDGTAGLARIKEQGGVT
                     IVQLPSDAEESSMPQAVITAGMADFVMSAAQIPQKLVELRDITQALHQIGRSPGPLAP
                     EAPQAGREAEVLDGIFTVLHARTGHDFHKYKRPTLMRRLERRLQVRGLTDLDSYYQLL
                     KKDSSEAHALLKDLLIGVTSFFRDRKAFDALEQQAVAQILRASPDGAEVRAWVAACST
                     GEEAYSVAMLLNDKAQTMGLQRKIQVFASDIDEQAIHTARAGLYPPGIADDVSPDRLR
                     RYFTMEGERYRVRKTLRDQVLFATHNLLRDPGFSRLDLITCRNFLIYLNREMHQYVLE
                     TFHFALNPGGFLFLGSAESADAASHCFAPVDLRHRIYRAKALPRAEFRVPLPPTVAPK
                     PLAPDGSQHQDFSARSRRPSSYADIHLRKLIELAAPSILL"
BASE COUNT         1035 a         2064 c         2015 g          990 t
ORIGIN      
        1 gcgctgtcgc tcgatcatct cgacctggcc gacatcgccg ccggcatcgt cgaggcgagc
       61 aaggaagacc cggccgctcg cgatcgccac atcacggtgg aaacgagcga gccgctgtgg
      121 gtgcatgcgg acaggatccg catcgagcag gtgatcctca atctggtgaa caacgcgatc
      181 aagttcaccg cgccgggcgg ccatatccgc gtcaccttga agcgcgccgg aagggaggcg
      241 gtgttggagg tcaaggacga cggccggggc atcgacacgg acttcctgcc gcatgtgttc
      301 gacatgttcc gccaggggcg caccggcccg aaccggtcca acagcgggct gggcatcggc
      361 ctggcgctgg cacgccagat cgttgaactg cacaacggcg tcatcaccgc cgagtcgggt
      421 ggcaacgggc gcggcgcctg cttctgcgta cgcctgccgc tgtccgatgc gccgcgcaac
      481 accggcgacg acgccgacca gggcgccggc caggccttgc acggtgtcga ggttcttctg
      541 gtcgacgaca acgccgaagc agtggacagt ttcaagtacc tgctggaatt cgagggcgcg
      601 aaggcgcacg tcacccgcac cgcccaggac gcgctcgcct gcctggacaa gcagcgcgtc
      661 gacctgatca tctccgatct ggcgatgccc ggcatggatg ggttcgcgct catggagcag
      721 gtgcgtgcgc gctacggcaa gaaatcgccg ctggccatcg cggtcagcgg gctgggccgc
      781 gcgcaggacg aagagcgcgc actgcaggcg ggttttgccg cgcacctgac caagccggtg
      841 tcgatcgata tgctgatgca gaccgcggcg aagctcttgg gcaagggctg atgcgcgccg
      901 ccaaggcggc ctgcaatttt ctccgcgttt cagggtcaaa aagccatccc ggcgagcctg
      961 ccgctgcagg ctcgccatgc ggccacgagg acccccttgc ccatacgctc cagaatcgtc
     1021 gtcatcggca cgtcggccgg tggacccgac gcgctgcgcc ggctgctccg gcggctcccc
     1081 gccgattttc cggccgccat cctcgcggtc atgcatatcg gcagccacta cagcacgctg
     1141 ccgcggattc tgcagcgcga tgccagcatg ccggtacgtc atgccgagga tggcgcgccg
     1201 atcgaacccg gcacggtgct gctcgccccg ccggatcgcc acctgatggt ggaagacggc
     1261 accacgcggc tttcgagcgg cccgaaagag aatttttccc gccccgcgat cgatccgctg
     1321 ttccgtaccg ccgcgctgtt ctaccgcgag aacgcgatcg gcctggtgct gaccgggctg
     1381 ctcgacgacg gcaccatcgg cctgcaggcg atcaaggcat acggcggcat cgcgatggtg
     1441 caggacccgc atgaagccga tgaaccggcg atgccgaaga gtgcgctgga gcatgtgcgc
     1501 gtcgatgcat gcctgccgct ggacgagctg gcggatcgtc tgctcgagct ggttcgatcc
     1561 gccccgcctc ccgtcgaggc ggggcgacag gtgaaggcga tcgaaaccca gggctggttc
     1621 gatctgcggc agaacacgga tttgacggaa ctcggtgaaa ttgccgtgcc gtcctcattt
     1681 acctgtccgg aatgcagcgg ctcgctatgg gaagtccggg acagcaatcc gccgtacttc
     1741 cgctgccata ccgggcacgc ctacaccgcg cagggattga acagcgcgca ggacatggcc
     1801 atcgaggaag cggtatgggc ggcgatccgc gcgctgcatg aaaagcagat gctgctgcat
     1861 cggctggccg gccaggccgc cagcgcgaat cgcgatgcgg cggtgcagga gcatgtcgcc
     1921 accgccgaaa cggtcgcgcg ccatgccgag atcctgcgac gcgtcgttgc gcgccatgac
     1981 tggcctgagg atttgacggg gtaggggctg cttgcgggcc gcctcgcgat gtcgtcgtcg
     2041 cgcaggcggc ggtgcgccag cgcagccaaa cgcgtttcac cgttcaccca agcgatgccc
     2101 cttcaccctg cgctggcccc gagcaggtgc tcgaactggc tgcgcgtcgc ggcatagcac
     2161 tcgcaggtcg tggcctcgag caggtcgcga cgcaggatgc gcatcatgcc gcggtgatac
     2221 gaaagcgcgc cttcctgctc gagccgacgc gcgatctggg agacgctggg ccgctgcacc
     2281 ccgagcatga aggacaggaa ttcctgcgtc attggaaaca cgtcctggcg caccctgtca
     2341 tgcgtcatca gcagccaacg cgcggtgcgc tgttccagcg tgtgcagctg gttgcacgcg
     2401 gccgattgct ccacctggga aatgtaggcc tgcgcgtaca ggtcgaggac ggtgcggaac
     2461 tgcgcattcg attccgcttc ccactgaaag tcgtcgatgc gcatgcgcag actgatgcct
     2521 gggatctggc agatcagtgt ctcggtcgcc acgtgtccgt cgagcagcgc gttgagcgac
     2581 gccatgccct cgttgcctat cgtgccgact tcgacggtcg cgccgtcggc catctccacc
     2641 agcgccgaat acacgctgct gcaggggaaa tcgacatggc gcaacggcgc gcccttcctg
     2701 atcagcacat gcttgacctc gacctcgtgc accgaaagca atggtgcgat atgcacgaac
     2761 tccggcgccg gcagcaattc cagaagacgg ttctggtgac gccacgtcag gtcgaccgct
     2821 tgcatagcgc tcccgttgga aatcggcgcg caggcgccac ccggccggca gttcacccac
     2881 gccgggcagt cagccgcgcg gacccgtggc gcccttgacg ccccgccgcc ggtcgcgcct
     2941 gatgtcggcc agcgcgcgca gaatctgttc gcggtgcagg tgcatggcgc tgaggccgtc
     3001 cagcagcagg gcgagcaggt cctcggaaga ccggcacgcg tggccggcct tgcgcagttc
     3061 ctcgatccgg tcgcgctgct cggcgatccg ccgctccgcc attgcgatat ggcggttcgc
     3121 cgcggcaagc tgcgcgcgtt cgtcaaccgg gcggggaaag gtcatcatca atgtgcgccc
     3181 gcgcaccggc cttgccgccg gatccggcga tgccgatgcg cctggtgttt ctgcatgcgc
     3241 tgtgagtcgc gtttcattca atggttcagt accgcggtcg catccgtctg gcgacgcgcg
     3301 agcagggtcg agcgtggcgg tgcaggcaat ccggaggaac gacaagccgc gcaataccgc
     3361 aagcgcggct ttgctgacgc ttcttatgtc agcgaccgaa cagaagcgcg cactgcgacg
     3421 gcggacaatg cctgcgacag gcgccgaacc cctggcacgg cgctcaagga gaagcgagat
     3481 ggatgtcatc gataccaatc tgcgtcaggc cgtcatccgg tccgaccccg ccgcgctcag
     3541 tgaatgcctg tggcgcggcg cagaggtcaa tgcgcgcgac gccaacgggt ggaccgccat
     3601 gcacttcgca tgcagttgtg cggacgcccg cattgccctg aagctgcttg cgcacggcgc
     3661 cgacgtgcag gttaacgaca agggcggcgt gggcccgctg catctggcgg cgatgcgtcg
     3721 tgacgcatcg ctggtgcggc tgctgctcga cgccggcgca ttcccggacg cccgggaccg
     3781 ctatggcgcc acaccgctgt ttctggctgc aggccggggc aacatcagcg cggtgaccgc
     3841 gctgctgcgc gccggcgcac gcgtggaccg cagcgacggg ctcggctaca acgcgctgca
     3901 ctgggcggct tccaaccgca atccgcgcac agcgcgcctg ctgctgcgcg cgggcgcgcc
     3961 gctgcacgcg gtggaaaacg aaggcatgac acccctgcac atcgcggtgc tgggcggtca
     4021 cctgcatacc gcacgcttcc tgattcagca gggcagcatt gtcgaccagc aggacgcggc
     4081 cgggcagacc gcgctgcatt tcgcggtgcg gcgttgcgac gccgagctgg cggcgctgct
     4141 gctgtctgcc ggggcaaaca tgcatgaagc aagtgccgcg gcggaaacgc cgttcgacgt
     4201 cgcctgctgg cagaacgacc cggtcgtgct ggacgttctg tacaggacgg aaacggcaaa
     4261 cgaagacagt tttttcgttg ccggcaatgc accgcaagcg gcaggcctgg agccctggca
     4321 gggcgacccg ctggcttgaa tccgcgaagc acgcaaggag tgtgacgcag ctcatcagtg
     4381 cgcacacgtt gcgaacttct gcttccgggc cggtggtcta cactttcagc ctgcctcaac
     4441 cggtaccgcc tgcttcccaa tgctcacgga gacagcccat accggcgccg tgcccgggcg
     4501 cgtcgcattt cctgtggtgg gcatcggcgc ttctgccggg ggcctgcccg ccctggtcac
     4561 gcttctggag aacatgccgt cttcgccggg catggcgctg gtggtcatcc tgcacctggc
     4621 gcccgatcag cccagcgcag tggaccgcgt gctgcagcgg gcaaccgaca tggcggttgc
     4681 ccaggccggc cagcgcgagt tcatcatgcc ggaccatgtc tacgtgattc cgcccgaccg
     4741 cagcctgagg atggaggatg gctacctcta cgtggaaccg ctggaccatc cggcgcacgg
     4801 gcgtctggtc accatcgacc ggttcttccg cacgctcgcc gaggcccaga aggaacgtgc
     4861 gatcggcatc gtgctgtcgg gcatgggaac cgatggcacg gcgggccttg cgcgcatcaa
     4921 ggagcagggc ggcgtcacca tcgtgcaact gccgtccgat gccgaggaaa gcagcatgcc
     4981 gcaggcggtg atcacggccg gcatggcgga tttcgtgatg agcgccgcgc agattccgca
     5041 gaagctggtg gagctgcgcg acatcaccca ggcgcttcac cagattggcc gctcgcccgg
     5101 cccgctcgct ccggaggcgc cgcaagccgg tcgcgaggcc gaggtgctgg acggcatttt
     5161 caccgtcctg cacgcgcgca ccggccacga cttccacaag tacaagcggc ccacgctgat
     5221 gcgccggctg gaacggcgcc tgcaagtacg cgggttgacc gatctcgaca gctactacca
     5281 gctgctgaaa aaagactcca gcgaagcgca cgcgctgctg aaggacctgc tgatcggcgt
     5341 gaccagtttc ttccgcgacc gcaaagcctt cgatgcactg gagcagcagg cggttgcgca
     5401 gatcctgcgc gcaagtccgg acggtgccga ggtgcgcgcc tgggtcgcgg cgtgctcgac
     5461 cggcgaagaa gcctattcgg tggccatgct gctgaacgac aaggcgcaga cgatggggct
     5521 gcagcgcaag atacaggtct tcgcctcgga catcgacgag caggcgatcc acaccgcgcg
     5581 tgccggcctc tacccgcccg gcatcgccga cgacgtctcg cccgaccggc tgcgccgcta
     5641 tttcacgatg gaaggcgaac gctaccgggt gcgcaagacc ctgcgtgatc aggtcttgtt
     5701 cgccacccac aacctgctgc gcgacccggg cttctcccgc cttgacctga tcacctgccg
     5761 caacttcctg atctacctga accgggaaat gcatcagtac gtgctggaga cgtttcactt
     5821 cgcactcaat ccgggcggat tcctgttcct cggcagcgcc gaatcggccg acgcggcctc
     5881 gcactgcttt gcgccggtcg accttcggca ccggatctac cgggccaagg cgctcccgcg
     5941 cgccgagttc agggttccgt taccgccgac ggttgctcca aagcccctgg cgccggacgg
     6001 gagccagcac caggattttt ccgcacgcag cagacgccct tcctcctatg ccgacatcca
     6061 tctacgcaag ctgatcgaac tcgccgcgcc gagcatcctg ttga
//
LOCUS       DATLGF010000291        11541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_44535,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000291 DATLGF010000000
VERSION     DATLGF010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 11541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11541
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44535"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..1526)
                     /locus_tag="VM406_08875"
     CDS             complement(141..1526)
                     /locus_tag="VM406_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HVL76117.1"
                     /translation="MRAALTLLAAAVLSACSLAPKHELPPSPVATAYPVQTAAAQAPA
                     GWRDFFAEPRLQALIGSALEHNRDLRIAALRIEEARALYRVQSADLLPSVGVAASAGR
                     GRTSADLSPTGSARTAGNYQVGLSLTAFELDFFGRVRNLNQAALAQYLATEEARNAAQ
                     IALVAEVARAYLSERAFAEQQALAQRTLQSRAEGLDLARQRFEAGATSALDLRQNETL
                     YHSARVAVATLARQRAQAENALTLLVGQPLAGLPAPQSLAAFDIATGIPAGLPSDLLT
                     LRPDIRSAEQRLVAANANIGAARAAFFPRISLTAGIGTASTALSGLFEAGTGTWSFVP
                     QLLMPIFDAGRNTANLDLAGTRKHVAVAEYERSIQAAFREVADALVARETLEEQVAAQ
                     QAVLESQSVRLRLADERYRAGVASSLDLLDAQREVFQAEQALVQARLARLTNAVDLYR
                     ALGGGLKESGG"
     gene            complement(1574..4726)
                     /locus_tag="VM406_08880"
     CDS             complement(1574..4726)
                     /locus_tag="VM406_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVL76118.1"
                     /translation="MGTFFIDRPVFAWVIAIFILIAGALSITALPVAQYPSVAPPAVN
                     VTAIYPGATAETLDSSITSVIEQELNGAPGLMYIESQSQANGQAQITATFAPGTDIDM
                     AAVEVQNRLKRVESRLPQAVTQQGVQVTKARSNFLMVVSLSSSDGSMDPVALGDYLSR
                     NVLNEIKRVPGVGEAMLFGTERAMRIWIDPQRLVGLNLTPTDVANAIRTQNAQVASGT
                     LGELPAPGDMQVTAPIVVTGQLSTIEQFGNIILRANPDGSLVRLRDVARIELGGQAYA
                     TAARIDGQPTSAIGIQLSPTANALETATAVRARMDELSRYFPQGVKYEIPYDTSKFVR
                     ISIKNVVMTLLEAVVLVFLVMFLFLQNIRYTIIPTIVVPVALMGTFAVMLMFGFSINV
                     LTLFGMVLAIGILVDDAIVVVENVERIMSEEGLPPREATRKAMKQITGAIIGITLVLT
                     VVFIPMAFFSGAVGAIYRQFSLSMAASIAFSALMALSLTPALCATILKPVQKGHHLEK
                     RGFFGWFNRGFGRVTDTYQGLVGRMVLRTGRLMLLYLAIVGIVVYMFMRLPGSFLPNE
                     DQGYILTNIQLPAGASAARTEEVIKQVEGYLMQQEGVQHVIAVRGFSFAGSGQNVALT
                     FVPLKDWSERDAATSADAIAGRAFGALMQIRDAIVFALNPPPIPELGTATGFNFRLQD
                     RSGQGHAALLAARNQLLGMAGQSPLLAGVRPDGMEDAPQLQLDIDRDRASTLGVSFAD
                     INSTLSAALGSSYLNDFPNQGRQQRVIMQAEATQRLQPEDLQRLHVRNARGDMVPLSS
                     FTSARWTKGPVQLIRYNGYPAMKIAGQAAPGYSSGDAMAEMERLAGQLPPGFGFEWTG
                     QSREEQLSGAQMPMLMALSLLAVFLVLAALYESTSIPFSVMLVVPLGVLGALIGVTLR
                     GMPNDVYFKVGLITIIGLSAKNAILIVEFAKDLQAQGKDLLESTIEAARLRFRPIIMT
                     SFAFILGVVPLAFSSGAGSASQRAIGTGVMSGMLVGTFLGVLFVPVFFVAVRKIFKGS
                     ERQRKMYAAKMHGDPAEENV"
     gene            complement(4739..5932)
                     /locus_tag="VM406_08885"
     CDS             complement(4739..5932)
                     /locus_tag="VM406_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVL76119.1"
                     /translation="MRTIPALRRFSVAFLAVFALAQLSACGERSAHAQAQAPGGAPPP
                     MEVPVVTVAPERISVTDELPGRLEATRVAQVRARVPGIVQKRVFREGSEVKAGEVLFR
                     VDAGPYQASLNSAEAALAKAEANLAQANLKVQRFKPLVETNAISKQEFDDAQTAQKQA
                     AADVAAAKAARESARLNLGYATVTAPIAGRIGRALVTEGALVGQGEATPLAVIQQLDP
                     IYVNITQSAAEALQLRRAMANGQLQAMGKGGAKVTIVTEDGQEYPHAGKLLFSDLTVD
                     QSSGAITLRAEVPNPDHFLLPGMYVRARVEQAVNEKAIAVPQQAVQRDVNGAAVFVVG
                     AGDKVELRPVKTDAARNNMWIISEGLQPGDRVIVEGFQKLGPGAPVKPVPWKGAQPAT
                     AAAQQ"
     gene            6067..6756
                     /locus_tag="VM406_08890"
     CDS             6067..6756
                     /locus_tag="VM406_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HVL76120.1"
                     /translation="MPRATKEEALETRSRILDAAEDVFHRNGVAHTSLADVAAAAGVT
                     RGAVYWHFKNKADLFNAMCERVRLPMEEMISAAASEDTADPLAALRESALFCLRQATT
                     NPHSHKVFDIVFHRCEFVDELDPVHARQRDAMLHGRAQLATLIEHAVRRNQLPADLDI
                     PLAAAAFQSHVSGLMNVWLFVPQSFDLAGEAERLIDSAIGMLRYAPALRMATAGGSAD
                     DACRSCAGVQG"
     gene            complement(6792..7835)
                     /locus_tag="VM406_08895"
     CDS             complement(6792..7835)
                     /locus_tag="VM406_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HVL76121.1"
                     /translation="MPTAPRTAVAVASVALLSVGFAPAAASGLHERLEQCATIATDAA
                     RLQCYDGIVGAHPGPVPPSGAGPPVPPPEPPLSHGLGAPSRLAEHWELEPATRQGLFS
                     IRLHRENYVLLTYSSNPNEAPYRPFASLVPGAGVVEPVELAFQLGFKLKLAEAPLGAP
                     MDLWFGYTQRSHWQIANPEASNPFRATDYQPELMAVFPVDANVLGLRMRFLNLGIVHE
                     SNGQTATLSRSWNRVYVQAGFERGNVSVLARAWQRFRVWEVDENPDIVDYMGYGDVEV
                     TLRRGQHDFSALARYNLRTSRGALQLGWAFPLTQKAPNLKGYLQFFSGYGYSLIDYNH
                     RQNVLGIGLMIAQ"
     gene            complement(7941..8609)
                     /locus_tag="VM406_08900"
     CDS             complement(7941..8609)
                     /locus_tag="VM406_08900"
                     /inference="COORDINATES: protein motif:HMM:NF039641.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6064 family protein"
                     /protein_id="HVL76122.1"
                     /translation="MSEWWSYRLSNFLMFSPQVYHRLFETHNRSWWPLQLLALAFGAA
                     VLLTMLRGGAIAARVATAAMGAAWIFIGWAFFMQRFADIHIGGTWMAAAFALQGLLLL
                     GTAALSPWRFARDAVARSGVVMFAFALLFWPLLAFIEERPWQQAEVFGLVPDPTAIAT
                     LGLVLAGQRIAWWLVPIPLLWCVFSGATLWSLGEMHAFLAPALALAALALMLISLQYR
                     NRKS"
     gene            complement(8821..9729)
                     /locus_tag="VM406_08905"
     CDS             complement(8821..9729)
                     /locus_tag="VM406_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVL76123.1"
                     /translation="MDRRNFLQSSLALAGAAAFPAARAAEPAPLVRPIPASGEAMPVV
                     GLGTWITFNVGRDRALRASSTEVVRHFFAQGGRMIDSSPMYGSAQEVVGEALQVLGHP
                     RQLFSADKVWVAPGSRGPAQIETSRRNWGIARFDLLQVHNLLAWEAHLDTLFAMKEAG
                     QLRYVGITTSEGRRHDDIERIMRAHPIDFVQLTYNVLDREAEARLLPLAQERGIAVIA
                     NRPFRQGALIRAVQRHRLPAFAADIDCANWAQFLLKFIVSHPAITCVIPATTRVDHLL
                     QNMGAARGRQPDAQMRRRMIAAVEAL"
     gene            complement(9761..10510)
                     /locus_tag="VM406_08910"
     CDS             complement(9761..10510)
                     /locus_tag="VM406_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HVL76124.1"
                     /translation="MEPGKTIRFAAVGDIHCTERSSGDLRAFFAQAADASDALLLCGD
                     LTDYGTPEEAHVLAEALAAARVPVVAVLGNHDYESGMQDEVRKILCDSGVRVLDGEAV
                     EVHGVGIAGAKGFAGGYGRRALGPWGESIIKEFVQEAIREALKLETALAKLRTDQRIA
                     MLHYSPIEATVQGEPVEIYCFLGTSRLEDPLLRYPVDAVVHGHAHRGTPEGRTVNNIP
                     VYNVAKPLLQRSFPDRPAFRIIEVPGKGAET"
     gene            10624..11442
                     /locus_tag="VM406_08915"
     CDS             10624..11442
                     /locus_tag="VM406_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HVL76125.1"
                     /translation="MSAVHSALSNGEGLDPVIAGFYRGVLAALEDSGRPYLIGGAYAL
                     NHYASIKRNTRDLDIFIKREDFDAVSAALLRAGFEAELTFPHWLGKIHAHGVYIDLIF
                     SSGNGVAVVDDAWFDHARTTELLGFEVKLSPIEEMIWSKAFIMERERYDGADIVHLLL
                     TRAEEVDWTRLMQRFDGHWRVLLAHLVLFGFVYPAERHWVPVWVMDELLRRLRSELKQ
                     PAPVGTLCQGTLLSREQYLPDIQEQGLRDGRILPHGNMTQEEAKLWTRAIPDRG"
BASE COUNT         1968 a         3859 c         3840 g         1874 t
ORIGIN      
        1 gggttcaagg gtctggttca gctgaggggt tcggctcttg tcgaggccgt tcgacttgct
       61 ccgaagtggt tccctccccc ttcaggggga gggaatctga cggggagtgt gcggactttc
      121 ggcaattcta gtccgcgcgc ctatcctccg ctttccttca acccgccgcc cagtgcccgg
      181 tacagatcca ccgcattcgt cagccgcgcc agccgcgcct gcaccagcgc ctgctcggcc
      241 tggaacacct cgcgctgcgc atcgagcaga tcgagagagc tggcgacgcc ggcgcgatag
      301 cgctcgtcgg ccagccgcag ccgcacgctc tgcgattcca gcaccgcctg ctgcgcggcc
      361 acctgctctt ccagcgtctc gcgcgcgacc agcgcatcgg caacctcgcg gaaggcggcc
      421 tggatcgagc gttcgtactc ggccacggcc acatgcttgc gcgtgccggc cagatcgagg
      481 ttggcggtgt tgcggccggc gtcgaagatc ggcatcagca gctgcggcac gaaggaccag
      541 gtgcccgtgc ccgcttcgaa caggcccgac agcgcggtgc tggcggtgcc gatcccggca
      601 gtgagcgaaa tgcgcgggaa gaaggccgcg cgggcggcgc cgatgttggc attggcggcg
      661 accaggcgct gctcggcgga gcggatgtcc ggacgcagcg tcagcaaatc cgacggcagg
      721 ccggcgggaa tgccggttgc gatgtcgaat gcggcgagcg actgcggcgc cggcaagccg
      781 gccagcggct gaccgaccag gagcgtcagc gcgttctcgg cctgcgcgcg ctggcgcgcg
      841 agcgtcgcca ccgccacccg ggccgagtga tacagcgttt cgttctggcg caggtcgagc
      901 gccgaggtgg cgccggcttc gaagcgctgc cgtgccagat ccagtccttc cgcgcgcgac
      961 tgcagcgtgc gttgcgccaa tgcctgctgt tcggcgaacg cgcgctcgga cagataggcg
     1021 cgcgccactt cggcaaccaa tgcgatctgg gcggcgttgc gcgcctcctc ggtagcgaga
     1081 tattgggcca gtgccgcctg gttgagattg cgcacgcggc cgaagaagtc cagctcgaac
     1141 gcggtcagcg acaggccaac ctggtagttg ccggcggtgc gcgcgctgcc ggtcggcgac
     1201 agatcggccg aggtgcgtcc tcgccctgcc gaggcggcca cgcccacgct cggcagcagg
     1261 tcggccgact gcacccggta cagcgcgcgc gcctcctcga tgcgcagcgc ggcgatgcgc
     1321 aggtcgcggt tgtgttcgag cgcggagccg atcagcgcct gcagccgcgg ctcggcgaag
     1381 aaatcgcgcc agccggccgg cgcctgcgcc gctgcagtct gcactggata ggcggtggcc
     1441 acaggcgacg gcggcagctc gtgcttcggc gccagcgaac aggcggacag gaccgcggcg
     1501 gccaagagcg tcagcgccgc gcgcatcggg ccgcgcatgg ccggctgcgc cagggttgcg
     1561 atagcgtgat tcatcatacg ttttcctccg cgggatcacc gtgcatcttg gctgcataca
     1621 tcttgcgctg gcgttcgctg cccttgaaga tcttgcgcac cgcgacgaag aacaccggca
     1681 cgaacagcac gccgaggaag gtaccgacca gcatgccgct catcacgccg gtgccgatcg
     1741 cgcgctggct cgccgagccg gcgccgctgg agaaggccag cggcaccacg ccgaggatga
     1801 acgcgaacga ggtcatgatg atcggacgga agcgcaggcg cgccgcctcg atggttgact
     1861 ccagcaagtc cttgccctgc gcctgcaggt ccttcgcgaa ctcgacgatc aggatcgcgt
     1921 tcttcgccga caggccgatg atggtaatca accccacctt gaagtacacg tcgttcggca
     1981 tgccgcgcag ggtcacgccg atcagcgcac cgagcacgcc cagcgggacc accagcatca
     2041 ccgagaacgg gatcgatgtg ctttcgtaca gcgcggccag caccaggaac acagccagca
     2101 gggacagcgc catcagcatc ggcatctgcg cgccggacag ctgctcctcg cgcgactgcc
     2161 cggtccactc gaaaccgaag ccgggcggca actggccggc caggcgctcc atctccgcca
     2221 tcgcgtcgcc gctgctgtag ccgggcgccg cctgtcctgc gatcttcatt gccggataac
     2281 cgttgtagcg gatcagctgc accggtccct tggtccagcg tgcggacgtg aaggacgaga
     2341 gcggcaccat gtcgccgcgt gcgttgcgca cgtgcaggcg ctgcaggtcc tcgggctgca
     2401 ggcgctgcgt ggcctcggcc tgcatgatca cgcgctgctg gcgtccctgg ttcgggaagt
     2461 cgttcagata gctggacccg agcgcggccg acaacgtgct gttgatgtcg gcgaacgaca
     2521 cgccgagcgt gctggcgcgg tcgcgatcga tgtcgagctg cagctgcggc gcatcttcca
     2581 tcccgtcggg gcgcacgcct gccaggagcg ggctttggcc cgccatgccg agcaactggt
     2641 tgcgtgcggc cagcagcgcc gcgtggccct gcccgctgcg gtcctgcaaa cggaagttga
     2701 agccggtggc ggtgcccagt tccgggatcg gcggcggatt gagcgcgaac acgatcgcgt
     2761 cgcggatctg catcagcgcg ccaaacgcgc gccccgcgat cgcatcggcg ctggtggcgg
     2821 cgtcgcgctc gctccagtcc ttcagcggca cgaaggtgag cgccacgttc tggccgctgc
     2881 cggcgaacga gaagccgcgc accgcgatca cgtgctgcac gccctcctgc tgcatcaggt
     2941 agccctcgac ctgcttgatc acctcttcgg tacgcgcggc cgatgcgccg gccggcaact
     3001 ggatgttggt gaggatgtaa ccctggtctt cattcggcag gaaggaaccg ggcaggcgca
     3061 tgaacatgta gaccacgatg ccgacgattg ccaggtacag cagcatcagg cggccggtac
     3121 gcagaaccat gcgccccacc aggccctggt aggtgtcggt cacgcgcccg aagccgcggt
     3181 tgaaccagcc gaagaagccg cgcttctcca ggtgatggcc tttttgcacc ggcttgagga
     3241 tggtcgcgca cagcgccggg gtcagcgaga gcgccatcag cgccgagaac gcaatggagg
     3301 ccgccatcga cagcgagaac tggcgataga tcgcgccaac cgcgccggag aagaaggcca
     3361 tcgggatgaa caccacggtc agcaccagcg tgatgccgat gatcgcgccg gtgatctgct
     3421 tcatcgcctt gcgggtcgct tcgcgcggcg gcagtccctc ctcgctcatg atgcgctcga
     3481 cgttctccac cacgacaatc gcgtcgtcga ccaggatgcc gatcgccagc accatgccga
     3541 acaaggtcag cacgttgatc gagaagccga acatgagcat gacggcaaag gtgcccatca
     3601 gcgcgaccgg cacaacgatg gtcgggatga tggtgtagcg gatgttctgc aggaacagga
     3661 acatcaccag gaacaccagc accacggctt cgagcagcgt catcacgacg ttcttgatcg
     3721 agatgcgcac gaacttcgag gtgtcgtacg ggatctcgta cttgacgcct tgcgggaagt
     3781 agcgcgacag ctcgtccatg cgtgcgcgca ccgcagtcgc ggtctccagg gcgttggccg
     3841 tcggcgagag ctggatgccg atcgccgagg tcggctggcc gtcgatgcgc gcggcggtcg
     3901 cataggcctg cccgcccagc tcgatgcgcg cgacatcgcg caggcgcacc agcgagccgt
     3961 ccggattggc gcgcaggatg atgttgccga actgctcgat ggtggagagc tggccggtga
     4021 cgacgatcgg cgcggtaacc tgcatgtcgc ccggcgcggg cagttcgccg agcgtgcccg
     4081 aagcgacctg ggcgttctgc gtgcggatcg cgttggccac gtcggtgggc gtcaggttga
     4141 gccccaccag gcgctgcgga tcgatccaga tgcgcatcgc gcgctcggta ccgaacagca
     4201 tcgcttcgcc aacgcccggg acccgcttga tctcgttgag cacgttgcgc gacagatagt
     4261 cgccgagcgc gaccggatcc atgctgccgt cggaggacga cagcgacacc accatcagga
     4321 aattggaacg cgccttcgtg acctgcacgc cctgctgggt cacggcctgc ggcaggcgcg
     4381 actcgacgcg cttcaagcgg ttctgcactt cgacggcggc catgtcgatg tcggtgcccg
     4441 gcgcgaacgt cgcggtgatc tgggcctggc cgttggcctg gctctgcgac tcgatgtaca
     4501 tcaggcccgg cgcgccgttg agctcctgct cgattacgct ggtgatgctg ctgtcgaggg
     4561 tctcggccgt tgcgcccgga tagatggcgg tgacgttgac tgcgggcggg gccacgctcg
     4621 gatattgcgc caccggcaat gcggtgatcg acagcgcccc ggcgatcagg atgaagatcg
     4681 cgatcaccca ggcaaagacc gggcggtcaa tgaagaaggt acccatggat gactctcttt
     4741 actgttgcgc ggcggcggtg gcgggctggg cgcccttcca cggcaccggc ttcaccggcg
     4801 cgccgggacc gagcttctgg aagccctcga cgatcacgcg gtcgcccggc tgcagtcctt
     4861 cgctgatgat ccacatgttg ttgcgcgcgg catcggtctt gaccggacgc agctcgacct
     4921 tgtcgccggc gccgaccacg aataccgcgg cgccgttgac gtcgcgctgc accgcctgct
     4981 gcggcaccgc gatcgccttt tcattgaccg cctgctcgac gcgggcgcgc acatacatgc
     5041 ccggcagcag gaaatgatcc gggttcggca cttccgcgcg cagcgtgatc gcgcccgagc
     5101 tctggtccac cgtgaggtcc gagaacagca gcttgccggc atgcgggtat tcctggccgt
     5161 cttcggtgac gatcgtgacc ttcgcgccgc ccttgcccat cgcctgcagc tggccgttgg
     5221 ccatcgcgcg gcgcaattgc agcgcttcgg cggcggactg ggtgatgttc acgtagatcg
     5281 gatccagttg ctggatcacg gccagcggcg tcgcctcgcc ctgcccgacc agcgcgcctt
     5341 cggtgaccag cgcgcggccg atgcggccgg cgatgggcgc ggtgacggtc gcatatccaa
     5401 ggttcaggcg cgcgctttcg cgtgcggcct tcgccgcggc cacgtcggcg gccgcctgct
     5461 tctgggcggt ctgcgcatcg tcgaattcct gcttgctgat cgcattggtc tcgaccagcg
     5521 gcttgaagcg ctgcaccttc agattggcct gtgccagatt cgcctcggcc ttggccagcg
     5581 cggcctccgc gctgttgagg ctggcctggt acggaccggc gtcgaccctg aacagcacct
     5641 cgccggcctt cacctcgctg ccttcgcgga acacgcgctt ctgcacgatg ccgggcacgc
     5701 gcgcacgaac ctgggcgacg cgcgtcgcct ccagccggcc gggcagttca tcggtcacgg
     5761 agatccgctc gggcgcgacg gtcaccaccg gcacttccat cggcggcggc gcgccgccgg
     5821 gcgcctgcgc ctgcgcatgc gcgctgcgtt cgccgcaagc gctcagttga gcgagtgcga
     5881 acactgccag aaaggcaacg gagaatcggc gaagtgcggg aatggttctc atcggagata
     5941 tccagggctg gggcggcggc ggtaccgcag cgttcaggaa aacgaaaggc caaaacaatc
     6001 gtgtcgtcgg ccggaaagtc gggtattcta catacattct cgaatgtatg tatagcagag
     6061 atcaaaatgc cacgagcaac caaggaagag gcgctggaaa cccgcagccg cattctcgac
     6121 gctgcggaag acgttttcca ccgcaatggt gtcgcccata cctcgctggc cgatgtggcc
     6181 gccgcggccg gtgtgacgcg cggcgccgtg tattggcatt tcaagaacaa ggccgatctg
     6241 ttcaatgcga tgtgcgagcg cgtgcgcctg ccgatggagg aaatgatctc ggccgccgcc
     6301 agcgaagaca ccgccgatcc gctggccgcg ctgcgcgaga gcgccctgtt ctgcctgcgc
     6361 caggccacca cgaacccgca ctcgcacaag gtgttcgaca tcgtgttcca ccgctgcgaa
     6421 ttcgtcgacg aactcgaccc ggtgcatgcg cgccagcgcg acgcgatgct gcacggccgc
     6481 gcccagctcg ccacgctgat cgaacatgcg gtgcgccgca accagctgcc ggccgatctc
     6541 gacatcccgc tcgcggccgc cgcattccag tcccatgtct cgggactgat gaacgtctgg
     6601 ctgttcgtgc cccagagttt cgatctcgcc ggagaggcgg agcggctgat cgattcggcc
     6661 atcggcatgc tgcgctacgc gccggcgctg cgcatggcca cagccggcgg cagtgccgac
     6721 gacgcctgcc gcagctgcgc cggcgtgcaa ggctgaaacc gacgtcgcgc gccgtcttcc
     6781 ggacggccct gctattgcgc gatcatcagt ccgattccaa gcacgttctg cctgtggttg
     6841 tagtcgatca ggctgtagcc atagcctgaa aagaactgca gatagccttt caggttaggc
     6901 gccttctgcg tgagcggaaa ggcccagccc agctgcagcg ctccgcgcga ggtgcgcaag
     6961 ttgtagcgcg ccagcgcgga aaaatcatgc tggccgcgac gcaaggtcac ctcgacgtcg
     7021 ccatagccca tgtaatcgac gatgtcggga ttctcgtcca cttcccatac gcggaagcgc
     7081 tgccaggcgc gcgccagcac gctgacattc ccgcgctcga agccggcctg cacgtagacg
     7141 cggttccagc tgcgtgacag cgtcgcggtc tggccgttcg attcatgcac gatgccgagg
     7201 ttgaggaagc gcatgcgcag gccgagcacg ttggcatcga cgggaaagac cgccatcagt
     7261 tccggctggt agtcggtggc gcggaacgga ttcgacgctt ccggattcgc aatctgccag
     7321 tggctgcgct gcgtgtagcc aaaccacaga tccatcggcg cgcccagcgg cgcctcggcc
     7381 agcttgagct tgaagccgag ctggaacgcg agctccaccg gctccaccac gccggcgccg
     7441 gggacgagcg acgcaaacgg gcgatacggc gcctcgttcg ggttgctgct gtaggtgagc
     7501 agcacgtagt tctcgcgatg caagcgtatc gagaacagtc cctgccgggt tgccggttcg
     7561 agctcccaat gctcggccag ccgcgaaggg gcgccaaggc cgtgcgaaag cggcggctcc
     7621 ggcggcggca ccgggggacc ggctccggat ggaggtaccg gtcccggatg cgcgccgacg
     7681 ataccgtcgt aacactgcag gcgcgcggca tcggttgcaa tcgtcgcgca ctgttcaagc
     7741 cgttcgtgaa ggccgctcgc agcggcaggt gcgaatccga ccgacaacag cgcaacactc
     7801 gcgaccgcaa cggctgtcct gggggctgta ggcatgccgg gctccggtac ttggcagcca
     7861 cgccggctgc acgccacgca acgcgcaagc cgcgcaacac ggcgccgcct tccgcactct
     7921 ggacgactag cgacgccgcg ctacgacttc cggttccggt attggagcga gatcaacatc
     7981 agcgccagcg cggccagggc cagggccggc gcgagaaacg catgcatttc ccccaggctc
     8041 cacagcgttg cgccactgaa gacacaccag agcagcggaa tcggcaccag ccaccaggca
     8101 atgcgttgcc cggcgagcac gaggcctaag gtcgcaatgg cggtcgggtc gggcacgagg
     8161 ccgaatactt cagcctgttg ccacgggcgc tcctcgatga acgccagcag cggccagaac
     8221 agcagcgcga acgcgaacat caccacgccg ctgcgcgcca ccgcgtcgcg tgcgaagcgc
     8281 cacggcgaga gcgcggccgt gccgagcagc agcaagccct gcaacgcgaa cgctgctgcc
     8341 atccaggtac cgccgatgtg aatatcggcg aagcgttgca tgaagaaagc ccagccgatg
     8401 aagatccatg ccgcccccat cgctgccgtg gcgacgcgcg cggcaatcgc gccgccgcgc
     8461 agcatcgtga gcaggaccgc cgcgccgaaa gccagcgcca gcaattgcag cggccaccag
     8521 ctgcggttgt gcgtctcgaa cagccggtgg tagacctgcg gcgagaacat caggaagttc
     8581 gacaggcggt aggaccacca ttcgctcatg gccgggctcc cggcgattgg gggtgcgaag
     8641 tggggtgcaa aggggggtgc gaagggccgg cgttgctgcg cgcgttgtga tgaacgccgc
     8701 tgggccccgg cttgcgccgg ggcgacagtg acccttcaag cgccatcgcc ctggccaacg
     8761 ccggggccga gcggcgttgt cgtccgctcc tgacaatgcc cttgcatgcg cacacgccgt
     8821 tcacaacgcc tccaccgccg caatcatgcg ccggcgcatc tgcgcgtccg gctggcgccc
     8881 gcgcgccgcg cccatgttct gcagcaggtg atcaacccgc gtggtcgcgg gtatcacgca
     8941 ggtgatcgcc ggatgcgaga cgatgaattt cagcaggaac tgcgcccagt tcgcgcaatc
     9001 gatgtcggcg gcgaatgccg gaaggcgatg ccgctgcact gcacggatca atgccccctg
     9061 gcgaaacggc cggttcgcga tcaccgcaat cccgcgttcc tgcgccagcg gcaacagccg
     9121 cgcctcggcc tcgcggtcga gcacgttgta ggtgagctgt acgaaatcga tcggatgcgc
     9181 gcgcatgatg cgttcgatgt cgtcatgccg gcgtccttcg gaagtcgtga tgccgacata
     9241 gcgcagctga cccgcctcct tcatcgcaaa cagcgtgtcc aggtgcgcct cccaggcgag
     9301 caggttgtga acctgcagca ggtcgaagcg ggcgatgccc cagttgcggc gcgaggtttc
     9361 gatctgcgcc ggaccgcggg aaccgggcgc gacccagacc ttgtcggcgg aaaacaactg
     9421 gcgcggatga ccgagcacct gcagcgcttc gccgaccact tcctgcgccg aaccgtacat
     9481 cggcgacgaa tcgatcatcc gtccgccctg cgcgaagaaa tggcgcacca cctcggtaga
     9541 ggatgcgcgc agtgcgcgat cgcgtccgac attgaaggtg atccaggtgc cgaggccgac
     9601 gaccggcatc gcttcaccgc tggccggaat cggccgcacg agcggggccg gttcggccgc
     9661 ccgcgcggcg ggaaaggcgg ccgcgcctgc cagcgcgagc gaggactgga gaaagttgcg
     9721 gcggtccatg gtcagaacga atggcttcga cccgcgaagc tcaggtctct gcgccctttc
     9781 cgggcacctc gatgatccgg aacgcggggc ggtcgggaaa gcttcgctgc agcaagggct
     9841 tggcgacgtt gtacaccgga atattgttca ccgttcttcc ctcgggcgtg ccgcggtgcg
     9901 catggccatg gacgaccgca tcgaccggat agcgcagcag cgggtcttcg agccggctgg
     9961 tgccgaggaa acagtagatc tcgaccggct cgccctgcac cgtcgcttcg atcggcgagt
    10021 agtgcagcat cgcgatgcgc tggtcggtgc gcaacttcgc caatgcggtc tcgagcttca
    10081 gcgcttcgcg gatcgcctcc tgcacaaatt ccttgatgat ggattccccc cacggcccga
    10141 gcgcgcgccg cccgtatccg ccagcaaatc cttttgcccc ggcaatgccg acgccatgca
    10201 cttcgaccgc ctcgccgtcg agcacgcgta cgccggaatc gcagagaatc ttgcgcacct
    10261 cgtcctgcat gccggattcg tagtcgtgat tgcccagaac cgccaccacc ggcacgcgcg
    10321 ccgccgccaa cgcctccgcc agcacgtgcg cttcctcggg cgtgccgtag tcggtcaggt
    10381 cgccgcacag cagcagtgcg tcggacgcgt ctgcggcctg tgcaaaaaat gcgcgcagat
    10441 ctcccgacga gcgctcggtg caatgaatgt cgccgacggc ggcaaatcgt atggtttttc
    10501 ctggctccac gctgactccc gttccttgtt cgtttgaatg atgatgcctg tgccattgtt
    10561 gactgtcatc gcgtggcata agttttgctg attcgcgtca tcatcaccca cgcgaggaac
    10621 atcatgagtg cagtccattc ggccctgtca aacggcgagg ggctcgatcc ggtcatcgcc
    10681 ggcttctatc gcggggtgct cgccgcgctc gaggattcgg gcaggcccta cctgatcggc
    10741 ggcgcgtatg cgctgaacca ctacgcaagc atcaagcgca acacgaggga tctcgacatc
    10801 ttcatcaagc gcgaggattt cgacgcggtt tcggccgccc tgctcagagc cggcttcgaa
    10861 gcggagctca ctttcccgca ctggctcggc aagatccacg cgcatggcgt gtacatcgac
    10921 ctgatcttca gttccggcaa cggcgtcgcg gtggtcgacg acgcgtggtt cgaccacgcg
    10981 cgcaccactg aactgctcgg cttcgaggtg aagctgtcgc cgatcgagga aatgatctgg
    11041 tcgaaagctt tcatcatgga acgcgaacgc tatgacggcg ccgacatcgt gcacctgctg
    11101 ctgacgcgcg ccgaagaggt ggactggaca cggctgatgc agcgcttcga cgggcactgg
    11161 cgcgtgctgc tcgcccacct ggtgctcttc ggcttcgtgt acccggccga gcgccattgg
    11221 gttccggtct gggtgatgga cgaactgttg cgtcggcttc gctccgagct caagcaaccg
    11281 gcgccggtcg gcacgctgtg ccagggaacc ctgctgtcgc gcgaacagta cctgccggat
    11341 atccaggaac aggggctgcg cgacggacgc atcctgcccc acggcaacat gacgcaggag
    11401 gaagcgaagc tgtggacgcg cgcgatcccc gatcgcggct gaatctacaa agcgatacgt
    11461 ttcgtcagca tgcaggagtg aacccgggtt gtgccgaaga gtctggaatg aggcaggtgt
    11521 gccgaacacc caacaacaag a
//
LOCUS       DATLGF010000292         2195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_28434,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000292 DATLGF010000000
VERSION     DATLGF010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28434"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..747)
                     /locus_tag="VM406_08920"
     CDS             complement(295..747)
                     /locus_tag="VM406_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HVL76126.1"
                     /translation="MDKNDVISTLNDLIQTCKDGVEGFRTCAEDISNPELKSYFNDRA
                     ERCTRAAEELQDLVVAYGGDPATSSHLSGTLHRRWVDVRSAITGKSDQDVLDECERGE
                     DVAKEHYRAALEKELPEDVRQIVEDQYRGVQINHDQVKGMRNQERARP"
     gene            complement(972..1664)
                     /locus_tag="VM406_08925"
     CDS             complement(972..1664)
                     /locus_tag="VM406_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76127.1"
                     /translation="MSSKTSNESAVATADETTRTAEIVREYGPFADGNTVNGVSYDGK
                     RVWAALGAKLVAFDPESGETTRTLDRPGDAGTAFDGKYLYQIAEARIDKIDPASGVVV
                     ASIPAPGNGNDSGLAWAEGSLWVGQYRDRKIHQIDPATGAIRRTIESNRFVTGVTWVD
                     GELWHGTWEGDDSDIRRIDPQSGAVLEQLKMPPGTGVSGLEADGAGLFYAGGGESGKV
                     RAVRRPRNDSRS"
     gene            complement(1716..>2195)
                     /locus_tag="VM406_08930"
     CDS             complement(1716..>2195)
                     /locus_tag="VM406_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVL76128.1"
                     /translation="VLFALAHALAEAWPGDVERGALIASAFRTQHADETMRARLRVEI
                     GRLRALLAPLADIEATGRGFALKPRNGRAVTVLAPPIDGDQAALLALLADGMPWSTSG
                     LALALGASQRTVQRALVELEAANRVRAIGRARAQCWLSPPLTGFTTILLLPASLPVV"
BASE COUNT          358 a          724 c          699 g          414 t
ORIGIN      
        1 agacggtcat cggcttcctg aaagaaaagc tgcccggtcc cgttgcctcg cagctcgacg
       61 gcgtggccgg cggcactggc gcaggaatga cggacaagat cggcggagtc ggatccagcc
      121 tcggcggcat gtttggcggc aaggacaaag gctagtgacc ggaagcgtgt gtgtttctgc
      181 cgcaggtctc gcgcctgcgg caatgctcac ggcgtccagg taacaatgcc gttcagcgcc
      241 tgactgaacg gcacgcgtga tgcgagacga atacccgcgt ccaacgggtt cagatcaagg
      301 ccgcgcgcgt tcctggttgc gcatgccctt tacctggtcg tgattgatct ggacaccgcg
      361 gtactggtct tccacgatct gccggacatc ctccggcagt tccttttcca gagcggctcg
      421 atagtgttcc ttggcgacgt cttcgccgcg ttcgcattcg tccagtacat cctgatcgct
      481 tttgccggtg atggcggaac ggacgtccac ccagcggcga tgcagcgtgc cactcaggtg
      541 cgaggatgtc gcgggatcgc cgccgtaggc gacgaccagg tcttgcagct cttccgctgc
      601 gcgggtgcag cgctcggcgc ggtcgttgaa gtaggacttc agttcgggat tgctgatgtc
      661 ttcggcgcag gtacgaaatc cttccacgcc gtccttgcag gtctggatca ggtcgttcag
      721 ggtcgagatg acgtcattct tatccattga ttcacctttt gtggttgaga aaatgtccgg
      781 catattgaag agacgacatg ctcaaggact gacgacagaa atcgggccga gttctctttt
      841 ttgcgtgggg agcgtatgac gctccggttt tggctggtct gcctgtccgg aaaatccggt
      901 tcagccatgc gaggacgaac caagacggtc atcacgcccg aagagtgggg cagaaggacg
      961 ccgccgattc ctcatgaacg cgagtcgttt cgcggccgac gcacggcacg caccttcccg
     1021 ctctcgccgc cgcctgcata gaacaggcct gccccgtccg cctccaggcc actgacgccc
     1081 gtgcccggcg gcatcttgag ctgttcgagc accgcgccgc tttgcggatc gatgcgccgg
     1141 atatcgctgt cgtcgccttc ccaggtgccg tgccacaact cgccatccac ccaggtcacg
     1201 ccggtgacga agcggttgga ttcgatcgtg cgccggatcg cgccggtggc cggatcgatc
     1261 tgatggatct tgcgatcacg atactggccg acccagagac tgccctccgc ccaggcgagc
     1321 ccggagtcgt taccgttacc cggggccggg atcgatgcca cgaccacgcc gctggccggg
     1381 tcgatcttgt cgatgcgggc ttcggcgatc tggtagaggt atttgccgtc gaaggcggtg
     1441 ccggcgtcac cggggcgatc gagcgtgcgc gtggtttcgc cgctctcggg atcgaaggcc
     1501 accagtttcg caccgagggc cgcccaaacc cgcttgccgt cgtagctcac gccattcacg
     1561 gtattgccat cggcgaaggg gccgtactcg cgcacgatct cggccgtgcg tgtcgtttca
     1621 tccgccgttg ccacggcgct ttcattgctg gtcttgctgc tcattacgct ctccttgtcg
     1681 gttgcagagc gccggtctgg cgctgcagga ccactctaaa caaccggcag tgaagccggg
     1741 agtaacaaga tcgtcgtgaa tccggtcagc gggggcgaca gccagcactg cgcccgtgcg
     1801 cggccgatgg cgcgcacccg gttggccgcc tccagttcca ccagcgcgcg ctgcacggtg
     1861 cgctgactcg cgcccagcgc cagcgccagc cccgaggtcg accacggcat gccgtccgca
     1921 agcagcgcca gcagcgcggc ctgatcgccg tctatcggcg gcgccagcac cgtgacggcg
     1981 cgcccattgc gcggcttgag cgcgaacccg cgcccggtgg cctcgatatc cgccagcggc
     2041 gcgagcagcg cacgcaggcg gccgatctcc acccgcaggc gcgcacgcat cgtctcgtcc
     2101 gcatgttgcg tacggaaggc gctcgcgatc aaggctcccc gctcgacatc gcctggccat
     2161 gcctcggcca gcgcatgcgc caaagcgaac agcac
//
LOCUS       DATLGF010000293         2302 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_28533,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000293 DATLGF010000000
VERSION     DATLGF010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28533"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..>2302
                     /locus_tag="VM406_08935"
     CDS             21..>2302
                     /locus_tag="VM406_08935"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVL76129.1"
                     /translation="MAVQAGESELNPAISAGPASTERSFLRRHGHVIFVWPLLGLLLT
                     VLLWIGTLSQLQRDHQQVEEGMVRHAESISRAYAEQLRRAVEQVDRITLSLKYDWEDP
                     DVVVDLEKQRGRGLYPDNSSLVASIIDREGRVVSTSGLDPSHLRVEADFFMHHMTDAR
                     DDLLISEAHVGRRVANNAIRFTRRINGPDGGFAGVVLVSVEPQHLVSFYDEAMLGAKD
                     FITLRRMDGPVLATRLGHGSRPAEIFYRSDPQFPGPSGVMREGAERFWDDEPRIVAWR
                     RLDGYPLVALAALSETSAFAAYRAGERRALAVATAGSVFLFLFTVVGMGFSARLAWRR
                     QQADEVKQTYRLAVDAAREGFYMFRALKNANGKLHDFQIQDCNERAATMLGTTRDALL
                     GARLSELYEGAYRDAELERMQRAMEGGYLEEEVRVPPESRLQAAWVHRRLVRSGAGLA
                     VTIRDVSEARAQQEALAAMANVDAVTGLPNRHWLGNFLPRALEQAKATDTRVAILFVD
                     LDDFKNINDTLGHAAGDELLKAAALRLKALVRASDHVVRLGGDEFTLVLQQVHRMDDV
                     ARVARLIVTALSEPFALGDTSGHTVQASVGISVYPDDGEDGETLLKHADIAMYAAKAG
                     GKGRYHFYHAQLSDTLLLRIQREQALRQAIERDEFVLHYQPRVDTATGRLLSVEALVR
                     WLHPERGMVPPLEFIQVAEDTGLILRLGRMVIEKACAQIAQWKAEGLPVVPVSINVSA
                     LQFNDGHVKDILADCMHRYRV"
BASE COUNT          369 a          743 c          809 g          381 t
ORIGIN      
        1 gccgcgtgca attcgagtgc atggcagtcc aagcgggcga atcagaattg aatccggcaa
       61 tttcggccgg gccggcgtcc accgagcgca gtttcctgcg ccggcacggg cacgtcattt
      121 ttgtctggcc cttgctgggt ctgctgctga ccgtgctcct gtggattggc acgctgtcgc
      181 agcttcagcg cgaccaccag caggtcgagg aaggcatggt gcgtcatgcc gaatcgatct
      241 cgcgcgccta tgcagagcag ttgcgccgcg ccgtcgagca ggtcgaccgg atcacgctga
      301 gcctgaagta cgactgggaa gacccggatg tggtggtcga tctggagaag cagcgcgggc
      361 gcggcctcta tccggataac tccagcctgg tggccagcat catcgaccgc gaggggcgcg
      421 tggtgagcac cagcggcctc gacccgtcac acctgcgcgt cgaggcggac ttcttcatgc
      481 atcacatgac ggacgcgcgc gacgacctgc tgatcagcga ggcccatgtc gggcgccgcg
      541 tggccaataa cgcaatccgc ttcacccgcc gcatcaacgg tcccgacggc ggcttcgcgg
      601 gtgtggtcct ggtttcggtg gaaccgcagc acctggtctc gttctatgac gaagcgatgc
      661 tcggcgccaa ggatttcatc acgctgcgcc gcatggacgg gccggtcctg gccactcgcc
      721 tcgggcacgg cagccgtccg gcggaaatct tctaccgcag cgatccgcag ttcccgggac
      781 ccagcggcgt gatgcgcgaa ggcgccgaac gtttctggga cgatgaaccg cgcatcgtcg
      841 cctggcgccg cctcgacggc tatccgctgg tcgcgctggc ggccctgtcg gagaccagtg
      901 cattcgccgc ctatcgcgct ggcgaacggc gcgccctggc ggtggcgacg gcaggcagcg
      961 ttttcctgtt cctgtttacc gtggtgggca tgggtttttc cgcacgcctg gcgtggcggc
     1021 gccagcaggc cgacgaggtc aagcagacct accgcctggc ggtcgacgcc gcacgggagg
     1081 ggttttacat gtttcgcgcg ctcaagaatg caaacgggaa actgcatgat ttccagatcc
     1141 aggactgcaa cgagcgtgcc gccacgatgc tcggcaccac gcgcgatgcg ctgctgggcg
     1201 cgcgcctgtc ggagctgtat gaaggcgcgt atcgggacgc cgagctggag cgcatgcagc
     1261 gtgcgatgga gggcggttac ctcgaagagg aagtccgcgt gccgccggag agccgcctgc
     1321 aggcagcctg ggtgcaccgc cgcctggtgc gctccggcgc cgggctggcg gtgacgatac
     1381 gcgacgtgtc ggaggcgcgg gcccagcagg aggcgctggc cgcaatggcc aacgtcgatg
     1441 cggtgaccgg gctgccgaac cgccattggc tcggcaattt cctgccgcgc gcgctcgagc
     1501 aggccaaggc gaccgacacc cgtgtggcga tcctgttcgt cgacctggac gacttcaaga
     1561 acatcaacga cacgctcgga cacgccgccg gcgacgagct gctcaaggcg gccgcgctgc
     1621 ggctcaaagc gctggtgcgc gcgagcgacc acgtggtgcg cctgggcggc gacgagttca
     1681 cgctggtgct gcagcaggtg caccggatgg acgatgtcgc gcgggtcgcg cggctgatcg
     1741 tcaccgcgct gtccgagccc tttgcgctcg gcgacaccag cggccacacg gtgcaggcct
     1801 cggtcggcat ctcggtctat ccggacgatg gcgaggacgg cgagaccctg ctcaagcatg
     1861 cggacatcgc gatgtatgcg gccaaggcgg gcggcaaggg gcgctaccac ttctatcatg
     1921 cgcagctttc cgacacgctg ttgctgcgca tccagcgcga gcaggcattg cgccaggcga
     1981 tcgagcggga cgaattcgtg ctgcattacc agccgcgcgt tgacaccgcc accggccggc
     2041 tgctgtcggt ggaggcgctg gtgcgctggc tgcatcccga gcgcgggatg gtgccgccgc
     2101 tggaattcat tcaggtcgcg gaggacaccg gcctgatcct gcgcctgggc cgaatggtga
     2161 tcgagaaggc ctgcgcccag atcgcgcagt ggaaggccga gggcctgccg gtggtgccgg
     2221 tgtcgatcaa cgtgtcggcg ctgcagttca acgacggcca tgtgaaggac atcctggccg
     2281 actgcatgca ccgctaccgg gt
//
LOCUS       DATLGF010000294         4463 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_101935, whole genome shotgun sequence.
ACCESSION   DATLGF010000294 DATLGF010000000
VERSION     DATLGF010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101935"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="VM406_08940"
     CDS             complement(<1..1146)
                     /locus_tag="VM406_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HVL76130.1"
                     /translation="MFTKILIANRGEIACRVAATAHRLGVKTVAVYSEADANAKHVAV
                     CDESVLIGPAPAKESYLRADKIIEVARATGAQAIHPGYGFLSENEDFAEACAKAGIAF
                     IGPPASAIRAMGSKSAAKALMEKANVPLVPGYHGDTQDADFLQEQADKIGYPVLLKAS
                     AGGGGKGMRIVEKSADFKAALESCKREAINSFGDDKVLVEKYLTRPRHIEIQVFADTQ
                     GNCIYLFERDCSVQRRHQKVLEEAPAPGMTEERRRAMGEAAVAAAKAVGYVGAGTVEF
                     IANQDGSFYFMEMNTRLQVEHPVTEMITGLDLVEWQLRVAAGEPLPLSQEQLRIDGHA
                     IEARICAENPDNNFLPATGTLKVYRKPPCSAFEPAVVRVDDGVREGDA"
     gene            complement(1372..2397)
                     /gene="bioB"
                     /locus_tag="VM406_08945"
     CDS             complement(1372..2397)
                     /gene="bioB"
                     /locus_tag="VM406_08945"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HVL76131.1"
                     /translation="MSTQTVVFQELATPAARREIEAQRWPVEKVVELFELPFNDLLWR
                     AQQVHRQHFDPQDVELATLLSIKTGGCPEDCGYCPQAARYDTGVKAQKLLELESVLAA
                     AQAAKDAGATRFCMGAAWRGPKDRDLDHVEAMVRGVKELGLEACATLGMLEDGQAERL
                     AAAGLDYYNHNLDTAPEFYNNIISTRDYDNRLDTLEKVRKAGIKVCCGGIVGMGESRR
                     QRAGLIAQVANMDPYPESVPINNLVQVEGTPLYGTEKIDPLEFVRTIAVARITMPKAR
                     VRLSAGRREMGEAVQALCFMAGANSIFYGEKLLTTGNPDVDADRELLKKLGMKTREVT
                     EEVHASE"
     gene            complement(2414..3124)
                     /gene="bioD"
                     /locus_tag="VM406_08950"
     CDS             complement(2414..3124)
                     /gene="bioD"
                     /locus_tag="VM406_08950"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HVL76132.1"
                     /translation="MKPAWFVTGTDTEIGKTLTTCTMLHALAQQGLRAAGMKPVAAGC
                     EERDGAWRNEDVDSIMAASSAGLPQQLVCPYLLREPAAPHIVAALEGVTIDPARILSS
                     FEQVRAQSDAVVVEGVGGFRVPLTNGYDTADLARDLKLPVVLVVGLRLGCINHALLTA
                     EAIAARGLTLAGWVANVVDLEMRLGMDNIEALTARLPAPLLGCIPRLPAALPSAAAAH
                     LDFSILPGWPQPSRPVSL"
     gene            complement(3121..4308)
                     /gene="bioF"
                     /locus_tag="VM406_08955"
     CDS             complement(3121..4308)
                     /gene="bioF"
                     /locus_tag="VM406_08955"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HVL76133.1"
                     /translation="MELLAQLEQDIAELDRRALRRRRRIAETPCAPHVMVDGRPMIAF
                     CSNDYLGLAAHPRVIEALREGASRYGAGSGASHLVSGHMRAHVVLEERLAAFVAPHLE
                     RPAALTFSTGYMANLAIVSGLAGRDADVFSEELNHASLIDGARLSRANVKVYPHRDVA
                     ALERLLAASTAKTKMVVTDSVFSMDGDIAPLPQMLALCERHGAWLVVDDAHGFGVLGA
                     NGRGALEHFGLRSPYLVYMGTLGKAAGVGGAFVAAHETVIDWLVQRARPYIFTTAAPP
                     ALTHALLASLDIIEGEEGRSRRVHLSALIAQVKSSLRLSRWHLHPSDTAVQPVIIGAN
                     DEALRAAAALHDEGYWVPAIRPPTVPAGTARLRITLSAAHTQEDVAALVRSVNRLEER
                     VSA"
     gene            complement(4311..>4463)
                     /locus_tag="VM406_08960"
     CDS             complement(4311..>4463)
                     /locus_tag="VM406_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     aminotransferase BioA"
                     /protein_id="HVL76134.1"
                     /translation="RFFTTALKHELLLRPIGNTVYLMPPYILSDGEIELLASRTRATL
                     DEVLGA"
BASE COUNT          706 a         1518 c         1492 g          747 t
ORIGIN      
        1 ggcatcaccc tcgcgcacgc cgtcgtccac gcgcacgacc gccggctcga aggcgctgca
       61 gggcggcttg cgatagacct tcagcgtgcc ggtggcgggc aggaagttgt tgtcggggtt
      121 ctcggcgcag atgcgggcct cgatggcgtg gccgtcgatg cgcagctgct cctgcgacag
      181 cggcagcggc tcgccggccg ccacgcgcag ctgccactcg accagatcca gcccggtgat
      241 catttccgtc accggatgct cgacctgcag gcgggtgttc atttccatga agtagaacga
      301 gccgtcctgg ttggcgatga actccacggt gccagcgccg acatagccga ccgccttggc
      361 cgcggccacc gcggcctcgc ccatcgcgcg gcggcgctct tcggtcatgc cgggcgccgg
      421 cgcttcttcc agcaccttct gatggcggcg ctgcaccgag cagtcgcgtt cgaacaggta
      481 gatgcagttg ccctgggtat cggcaaacac ctggatctcg atatgacgcg gacgggtcag
      541 gtacttctcg accagcacct tgtcgtcgcc gaagctgttg atcgcctcgc gcttgcagga
      601 ctccagtgca gccttgaaat ccgcgctctt ctcgacgatg cgcatgccct tgccgccgcc
      661 gccggcggac gccttcagca gcaccggata gccgatcttg tcggcctgct cctgcaggaa
      721 gtccgcatcc tgggtatcgc cgtggtaacc cggcaccagc ggcacgttcg ccttttccat
      781 cagggccttc gcggcagact tggagcccat tgcgcgaatc gccgaggccg gcggtccgat
      841 aaaggcgatc ccggccttcg cgcaggcttc ggcgaagtcc tcgttctcgg acaggaagcc
      901 atagcccgga tggattgcct gcgcgccggt ggcgcgcgcg acctcgatga tcttgtccgc
      961 gcgcagatag ctttccttgg ccggtgccgg gccgatcagg acggactcgt cgcagacggc
     1021 gacgtgcttg gcgttggcat cggcttcgga gtagacggcc accgtcttga caccgaggcg
     1081 atgggctgtg gcagcaacac ggcaggcgat ttcaccgcgg ttggcgatca ggattttggt
     1141 gaacatggtc tctatcgggg aatgtggttg ttatgtcctg tcaagttccc tcccccttcc
     1201 agggggaggg ttagggtgga ggtagtgagc ccgagctcgc gatagtgcaa gctcgaagtc
     1261 tctacccctc ctaacctccc cctacaaggg gggagggact agtccgtgca atgagaaagt
     1321 aatcgtccaa actcaagcga cgtgtgtgtc gcggaaggga acagcccgcc gtcactccga
     1381 cgcatgcacc tcttccgtca cctcacgcgt cttcatgccc agcttcttca gcagctcgcg
     1441 atccgcgtcc acatccgggt tgccggtcgt cagcagcttc tcgccgtaga agatcgagtt
     1501 cgctcccgcc atgaagcaca gcgcctgcac cgcctctccc atctcgcggc ggcccgccga
     1561 caggcgcacg cgcgccttcg gcatcgtgat gcgggcgacc gcgatcgtgc gcacgaattc
     1621 cagcgggtca atcttctcgg tgccatacag cggcgtgcct tcgacctgca ccaggttgtt
     1681 gatcggcacc gactccggat acggatccat gttcgccacc tgcgcgatca ggccggcgcg
     1741 ctgccggcgc gattcgccca tgccgacgat gccgccgcag cagaccttga tgccggcctt
     1801 gcgcaccttc tccagcgtat cgaggcggtt gtcgtagtcg cgcgtgctga tgatgttgtt
     1861 gtagaactcg ggcgcggtgt cgaggttgtg gttgtaatag tccaggcccg ctgcggcgag
     1921 gcgctcggcc tggccgtctt ccagcatgcc cagcgtcgcg caggcttcga gtcccagttc
     1981 cttcacgccg cgcaccatcg cttcgacatg atcgagatcg cggtccttcg gcccgcgcca
     2041 ggcggcgccc atgcagaagc gcgttgcgcc ggcatccttc gccgcctgcg cggccgccag
     2101 cacggattcc agctccagca gcttctgtgc cttcacgccg gtgtcgtagc gcgccgcctg
     2161 cgggcaatag ccgcaatcct ccggacagcc gccggtcttg atcgacaaca gcgtagccag
     2221 ctcgacgtcc tgcggatcga aatgctgacg gtgcacctgc tgcgcgcgcc acagcaggtc
     2281 gttgaacggc agctcgaaca gttcgacgac cttctccacc ggccagcgct gcgcctcgat
     2341 ttcgcggcgc gcggcggggg tggcaagttc ctggaatacg acggtttgcg tagacatcaa
     2401 cgcttccttt acatcagagt gagacgggac gggatggctg cggccagccc ggaagaatcg
     2461 aaaaatcgag atgcgcggcg gccgcggacg gcagggccgc gggcaggcgc ggaatgcagc
     2521 ccagtaatgg agcaggcagg cgcgcggtca gcgcctcgat gttgtccatg ccgagccgca
     2581 tctccaggtc caccacgttc gcgacccagc cggccaacgt caatccgcgc gcggcgatcg
     2641 cctccgcggt gagcagagca tggttgatgc agcccagccg cagcccgacc accagcacca
     2701 cgggcagctt cagatccctg gccaggtcgg cggtgtcgta gccgttcgtc agcggcacgc
     2761 ggaagccgcc gacgccctcc acgaccacgg cgtcggactg cgcgcgcacc tgctcgaagc
     2821 tcgacaggat gcgcgccgga tcgatggtga cgccttccag cgcggcgacg atatgcggcg
     2881 cggcgggctc gcgcagcagg tacgggcaga ccagctgctg cggcaggccg gcactggagg
     2941 cggccatgat gctgtccacg tcctcgttgc gccaggcgcc gtcgcgctcc tcgcagccgg
     3001 cggcgaccgg cttcatcccg gccgcgcgca ggccctgctg cgcgagcgca tgcagcatcg
     3061 tgcaagtggt cagggtcttg ccgatttcgg tgtcggtacc ggtgacgaac caggcgggtt
     3121 tcatgcgctc accctttctt caagacggtt gacggatcgc accagcgcgg ccacatcctc
     3181 ctgcgtatgc gcggccgaaa gcgtgatgcg cagacgggcg gtgccggccg gcaccgtcgg
     3241 cgggcggatc gccggtaccc aataaccctc gtcgtgcagc gccgcggcgg cacgcagcgc
     3301 ctcgtcgttc gcgccgatga tgaccggctg caccgcggta tcggacggat gcagatgcca
     3361 gcgcgacagg cgcagcgaac tcttcacctg cgcgatcagc gcggacagat gcacgcggcg
     3421 cgagcggccc tcctcgccct cgatgatgtc gaggctggcg agcagcgcat gcgtgagcgc
     3481 cggcggcgcg gcggtggtga agatgtacgg acgcgcgcgc tgcaccagcc aatcgatcac
     3541 ggtctcgtgc gcggcgacga aggcaccgcc gacgccggcc gccttgccga gcgtgcccat
     3601 gtagaccagg tagggcgagc gcagcccgaa atgctcgagc gcgccgcggc cgttcgcgcc
     3661 gagcacgccg aaaccgtgcg cgtcatcgac gaccagccag gcgccgtgac gctcgcacag
     3721 cgcaagcatt tgcggcagcg gcgcgatgtc gccgtccatg ctgaacacgc tgtcggtgac
     3781 caccatcttg gtcttcgcgg tgctggccgc gagcaggcgc tccagcgcgg ccacgtcgcg
     3841 atgcgggtag accttcacgt tggcgcgcga cagtcgcgcg ccgtcgatca acgaggcgtg
     3901 gttcagttcc tcggaaaaca cgtcggcgtc gcgcccggcc aggccggaga cgatcgcgag
     3961 attggccatg tagccggtgc tgaacgtcag cgccgccggc cgctccaggt gcggcgcgac
     4021 gaaggcggcg aggcgctcct ccagcaccac gtgcgcacgc atgtggccgc tgaccaggtg
     4081 cgatgcgccg ctgccggcgc catagcggct ggcaccctcg cgcagcgcct cgatcacgcg
     4141 cggatgcgcg gcaaggccga ggtagtcgtt gctgcagaac gcaatcatcg gccggccgtc
     4201 caccatcacg tgcggcgcgc acggcgtctc ggcgatgcgg cggcggcggc gcagcgcgcg
     4261 gcggtcgagt tccgcgatgt cctgttcgag ctgggcaagc agttccatcg tcaggctccc
     4321 agcacttcgt cgagcgtggc acgggtgcgg ctcgcaagca gctcgatctc gccgtcggag
     4381 aggatgtagg gcggcatcag gtagacggtg ttgccgatcg gacgcagcag cagttcgtgc
     4441 ttcaacgcgg tcgtgaagaa acg
//
LOCUS       DATLGF010000295         4958 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_150524, whole genome shotgun sequence.
ACCESSION   DATLGF010000295 DATLGF010000000
VERSION     DATLGF010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150524"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VM406_08965"
     CDS             <1..915
                     /locus_tag="VM406_08965"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HVL76135.1"
                     /translation="EGRMLVRNQQGIELLSNVCRHRQAKMFDGRGNARSIVCPLHRWT
                     YDLKGELIGAPHFDDTPCMNLSRTPLQGWNGLLFEQNGFDVAQAMSKLSVTKDLDFSG
                     YMLDHVEVHECNYNWKTFIEVYLEDYHVEPFHPGLGNFVTCSDLRWEFGEHYSVQTVG
                     VNHGLAKSGSPKYRKWQEQVMKFRDGVPPPYGAIWLTIYPNIMVEWYPHVLVVSTVWP
                     QGPEKTINTVEFYYPEEIVLFEREFVEAERAAYMETAVEDDEIAERMDAGRRILLARG
                     VNEMGPYQSPMEDGMQHFHEWYRGHIAV"
     gene            1056..2003
                     /locus_tag="VM406_08970"
     CDS             1056..2003
                     /locus_tag="VM406_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVL76136.1"
                     /translation="MQSLWMLFASFVFSVMGVCVKLASETYSIAEIITYRGLVGVVFM
                     YALIRWQGGTLRTALPWHHAWRGAVGVTALWMWFYSISELPIATGMTLNYMAPIWIAA
                     ILFTGGWLRRETGFEWGLAATILVSFVGVTLLLRPAFHADQWFAALIGLMSGILSALA
                     YLQVRKLGLLGEPEYRVVFYFSATGFLAGLVGTTTGFGMPAGASGWHAHTGSGLALLL
                     AIGITATVAQVAMTRAYRLGKPLLTANLQYSGILFSSLWGVLIWSDVLSWQSWAGMVV
                     ILGSGMAATWFGARRALVAARRRARQAPADGVAKPAGEG"
     gene            2042..2899
                     /locus_tag="VM406_08975"
     CDS             2042..2899
                     /locus_tag="VM406_08975"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HVL76137.1"
                     /translation="MQYTTLIGAAALANHLDDPRWVVIDCRHDLAQPDAGRSAYEAGH
                     IPSARFAHLDTDLSDKRPAPDGSFRGRHPLPERAAFAETLRGWGVNQDSQVVAYDAHG
                     GMFAARLWWMLRWAGHEAVAVLDGGIAAWTAIGQPLSTERPAPSPGNFELRAPLERTV
                     DATRLVANLAQPELAVIDARAPDRFRGENETLDPVGGHIPGARNRFFKDNLLPDGRFK
                     PAARLAAEFADLVKQPEQAVMQCGSGVTACHNLLALCVAGMGGAALYPGSWSEWCADR
                     SRPVASGDQ"
     gene            complement(2893..3576)
                     /locus_tag="VM406_08980"
     CDS             complement(2893..3576)
                     /locus_tag="VM406_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HVL76138.1"
                     /translation="MVENMVSLSVGILLSVALLHALPEAFMSEADAHVLFAVLLGGLL
                     SFFLLEKFAILRHSHHYEGDGHGHEHGHDRHEAGKAGWLILVGDGLHNFTDGILIAAA
                     FLVDPWLGLITSMAIIAHEIPQEIGDFIVLLNAGFSRTRAYVYNLLSSLMAIIGGIVG
                     FLALDRASDWIPYVLVFASSGFIYIAVSDLMPQMQRRATLRETVPQIVLVALGIAIVL
                     LLTSHAHNH"
     gene            complement(3804..4685)
                     /locus_tag="VM406_08985"
     CDS             complement(3804..4685)
                     /locus_tag="VM406_08985"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HVL76139.1"
                     /translation="MQDLKNDFDSLSKTTLSDAVARRDFLKVTLGAGFAAAVMPVAAQ
                     SVIKTDTAGLAAGTVTINVKGQDVPVYRAQPQGKTNLPVILVVPEIFGVHEHIADVAR
                     RFAKLGYLALAPELFVRQGDPSKYTSIPELQKEIISKVPDAQVMADLDGCVDWAARNG
                     GDTRRMGITGFCWGGRITWLYAAHNPMVRAGVAWYGRLVGQQSELTPRHPVDVAPKLT
                     VPVLGLYGGKDSGIPLDSVEKMKAALAEGKSGSDFVIYDNAGHAFHADYRPSYVEADA
                     KDAWQRCVAWFKRHGVA"
     gene            complement(4705..>4958)
                     /locus_tag="VM406_08990"
     CDS             complement(4705..>4958)
                     /locus_tag="VM406_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HVL76140.1"
                     /translation="APMQGTAADLIKLSMIAVQDWLEAQKLGSKMIMQVHDELVLEVP
                     EAELPAIRDNLPALMQGVAALNVPLIAEVGVGRNWEEAH"
BASE COUNT          847 a         1633 c         1610 g          868 t
ORIGIN      
        1 gagggccgca tgctggtgcg caaccagcaa ggcatcgaac tgctgtccaa cgtctgccgc
       61 catcggcagg ccaagatgtt cgacggccgc ggcaatgcac gctccatcgt ctgtccgctg
      121 catcgctgga cctacgacct caagggcgaa ctgatcggcg cgccgcactt cgacgacacg
      181 ccctgcatga acctgtcgcg cacgccgctg cagggctgga acggcctgct gttcgagcag
      241 aacggattcg acgtcgcgca ggcgatgtcc aagctgtcgg tgacgaaaga cctggatttc
      301 tccggctaca tgctcgacca cgtcgaagtc catgagtgca attacaactg gaagaccttc
      361 atcgaggtct acctggagga ctaccacgtc gagcccttcc accccggcct tggcaatttc
      421 gtcacctgct cggatctgcg ctgggagttc ggcgaacact acagcgtgca gacggtcggc
      481 gtgaaccacg gcctggcgaa gtcaggctcg ccgaaatacc ggaagtggca ggaacaggtc
      541 atgaagttcc gcgacggcgt tcccccgccg tacggcgcga tctggctcac catctatccg
      601 aacatcatgg tcgagtggta tccgcacgtg ctggtggtct ccaccgtctg gccgcaggga
      661 ccggagaaga cgatcaatac cgtggagttc tactacccgg aggaaatcgt gttgttcgag
      721 cgcgagttcg tcgaggccga gcgcgccgcc tacatggaga ccgcggtcga ggacgacgag
      781 atcgccgaac gcatggacgc agggcgccgc atcctgctgg cgcgcggcgt caacgaaatg
      841 gggccgtacc agtcgccgat ggaagacggg atgcagcact tccacgagtg gtatcgcggg
      901 cacatcgcgg tctgacgaga cgcgcagcgt agccgaccaa cggggccaag cggccccgtt
      961 ttttttcgct ctgcagatca tggcgaacgg gcgtatcatc ccgatcctcg atttccccca
     1021 agaaatctcc cctccctgcc atttcgcccc ttcccatgca gtcgctctgg atgttgtttg
     1081 caagcttcgt gttttccgtg atgggcgtgt gcgtcaagct tgcctccgaa acctattcga
     1141 ttgcagaaat catcacctac cgcggactgg tcggcgtggt gttcatgtat gcgctgatcc
     1201 gctggcaggg cggcacgctg cgcaccgcgc tgccgtggca tcacgcgtgg cgcggtgcgg
     1261 tcggcgtgac cgcgctgtgg atgtggttct actcgatcag cgagttgccg attgcaaccg
     1321 gcatgacgct caattacatg gcgccgatct ggatcgccgc cattctcttc accggcggct
     1381 ggctgcggcg cgagaccgga ttcgagtggg ggctggcggc caccatcctg gtcagcttcg
     1441 tcggcgtgac gctgttgctg cggccggcct ttcatgccga tcaatggttt gccgcgctga
     1501 tcggcctgat gtccggcatc ctgtccgcgc tcgcgtatct gcaggtgcgc aagctcggct
     1561 tgctcggcga gccggagtac cgggtcgtgt tctatttttc cgcgaccggt tttctggccg
     1621 gattggtcgg caccacgacc ggttttggca tgccggcggg cgcctcggga tggcatgccc
     1681 ataccggcag cgggctcgcg ctgctgctgg ccatcggcat caccgccacg gtcgcccagg
     1741 tcgcgatgac gcgcgcttac cgcctcggca agccgctcct gaccgcgaat ctccagtaca
     1801 gcggcatcct gttctccagc ctgtggggtg tgctgatctg gagcgatgtg ctctcctggc
     1861 aaagctgggc cggcatggtg gtgatcctgg gcagcggcat ggccgccacc tggttcggag
     1921 cccgccgcgc cctcgttgcg gcacgccgcc gcgcgcggca ggcaccggcc gatggcgttg
     1981 caaagccggc cggagagggc taggctttgc gctccaggaa ccagccccgg tcggagcgca
     2041 catgcaatac acaaccctca tcggcgcggc agcactcgcg aaccatctcg acgacccgcg
     2101 ctgggtcgtg atcgactgca ggcacgacct cgcccaaccc gacgccggcc gcagcgccta
     2161 cgaagccggc catattccaa gcgcccgctt cgcccacctg gacacggatc tgtcagacaa
     2221 gcgtccggcg cccgacggca gcttccgcgg ccgtcatccg ctgccggagc gcgcggcctt
     2281 cgccgagacg ctgcgcggct ggggcgtcaa tcaggactcg caggtcgtgg cctacgacgc
     2341 gcatggcggc atgttcgccg cgcggttgtg gtggatgctg cgctgggccg ggcatgaggc
     2401 agtggcggtg ctcgacggcg gcatcgccgc atggactgcg atcggccagc ccttgtcgac
     2461 ggagcggccg gcgccctcgc ccggcaattt cgagctgcgc gcgccgctgg agcgcaccgt
     2521 cgacgcaacg cggctcgtcg ccaatcttgc ccaaccggaa ctggccgtca tcgacgcgcg
     2581 cgcgccggac cgcttccgcg gcgagaacga aacactcgac ccggtcggcg gccacattcc
     2641 gggcgcgcgc aaccgcttct tcaaggacaa cctgctgccg gatggccgct tcaaaccggc
     2701 tgcccggctc gccgccgagt tcgccgatct cgtcaagcag ccggagcagg ccgtgatgca
     2761 atgcggctcc ggtgtgacgg cctgtcataa cctgctcgcg ctatgcgtgg ccggcatggg
     2821 cggggcggca ctctacccgg gttcgtggag cgaatggtgc gccgaccgct cgcgccccgt
     2881 ggccagcggc gatcagtgat tgtgtgcgtg gctggtcagc agcagcacga tcgcgatgcc
     2941 gagcgcgacc aggacgatct gcgggaccgt ctccctgagc gtggcccgcc gctgcatctg
     3001 cggcatcagg tcgctgacgg cgatgtagat gaagcccgag gaagcgaaca ccagcacata
     3061 tggaatccag tctgacgcgc ggtccagcgc caggaaaccg acgatgccgc cgatgatcgc
     3121 catcaggcta gacagcaggt tgtagacgta cgcgcgggtg cggctgaagc cggcattgag
     3181 cagcacgatg aagtccccga tctcctgcgg aatctcgtgc gcgatgatcg ccatcgacgt
     3241 gatcaggccg agccaggggt cgaccaggaa ggccgcggcg atcaggatgc cgtcggtgaa
     3301 gttgtgcagg ccgtccccga ccaggatcag ccagccggcc ttgccggcct catggcgatc
     3361 gtggccgtgc tcgtgcccgt gcccgtcgcc ctcgtagtgg tgcgaatggc gcaggatcgc
     3421 gaacttctcc agcaggaaaa agctcagcag gccgccgaga agaaccgcga acagcacgtg
     3481 ggcgtcggcc tccgacatga aggcttcggg cagcgcgtgc agcagcgcga ccgagagcag
     3541 gatgccgacg gacaggctga ccatgttctc gaccatgcgc gagagcaggg aaaaggagaa
     3601 cagcgcggca gcggaaatac tgaggacgcc cgcgagggtg gtcgcgagca ggatgtagag
     3661 cagcgtgggg tcgatttccg gcctctaatt tatgcaacaa agttgcaaat taaagcacag
     3721 gcgcgtcatt tcagcaatga cgcgagcggg cgcccggaag cgcccgcctt gagaacggcc
     3781 ggtttcttcg ctggtttccc cgcttacgca actccgtgac gcttgaacca ggcgacgcag
     3841 cgctgccacg catccttcgc gtcggcctcc acgtagctgg ggcggtaatc ggcatggaac
     3901 gcgtggccgg cgttgtcgta gatcacgaag tcggatccac tctttccctc ggccagcgcc
     3961 gccttcatct tttccacgga atcgagcgga atgccgctgt cctttccgcc gtacaggccg
     4021 agcaccggca ccgtgagctt gggggcgacg tccacgggat ggcgcggcgt cagttcggat
     4081 tgctggccga ccaggcggcc gtaccaggcc accccggcgc gcaccatcgg gttgtgcgcc
     4141 gcatacagcc aggtgatgcg cccgccccag cagaaaccgg tgatgcccat gcgcctggtg
     4201 tcgccgccgt tgcgcgcagc ccagtcgacg cagccatcga gatcggccat cacctgcgca
     4261 tccggcacct tcgagatgat ctccttctgc agttccggga tgctggtgta cttcgatgga
     4321 tcgccctggc gcacgaacag ctccggcgcg agcgcgagat agccgagctt tgcgaagcgg
     4381 cgtgccacgt cggcgatatg ctcgtgcacg ccgaaaatct cggggaccac gagaatgacg
     4441 ggcaggttcg tcttgccttg cggttgggcg cggtagaccg gcacatcctg gcccttgacg
     4501 ttgatcgtta ccgtgcccgc ggcaaggccg gccgtgtcgg tcttgatgac gctctgggcg
     4561 gccaccggca tcacggccgc ggcaaagccg gcacccaggg tcaccttgag gaaatcacgc
     4621 cgcgcaacgg cgtcggaaag agtggtcttg ctgaggctgt cgaaatcgtt cttcaggtcc
     4681 tgcatcgcga tcctccggta taggtcagtg ggcttcctcc caattgcggc cgacgccgac
     4741 ctcggcgatc agcggcacgt tcagcgctgc gacgccctgc atcagggcag gaagattatc
     4801 gcggattgcc ggcaactccg cttccggcac ctccagcacc agttcgtcat gcacctgcat
     4861 gatcatcttc gaacccagct tctgcgcctc cagccaatcc tgcacagcga tcatcgacag
     4921 cttgatcagg tcggccgccg tgccctgcat cggcgcat
//
LOCUS       DATLGF010000296        11954 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_44969,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000296 DATLGF010000000
VERSION     DATLGF010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 11954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11954
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44969"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1629
                     /locus_tag="VM406_08995"
     CDS             <1..1629
                     /locus_tag="VM406_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVL76141.1"
                     /translation="TNVQLPLDHPPADLQAAILARLQIAPEQLLKVNVFRRGYDARRR
                     SAITLVYTLDVEVEDEQGVLARLAGVPGVGIAPDTSYKFVARAPAALRKRPVVIGFGP
                     CGIFAALILAQMGFRPIILERGKVVRERTKDTWGLWRRRELNPESNVQFGEGGAGTFS
                     DGKLHSGIKDPKHYGRKVLTEFVNAYAPEEILYVSKPHIGTFRLVKMVEAMRATIESL
                     GGEFRFEAKVSDIAIENGRVRGVQIENGDFIEADHVVLAVGHSARDTFQMLHERGVYI
                     EAKPFSIGFRIEHPQSMIDRARFGKFAGHPLLGAADYKLVHHAKNGRAVYSFCMCPGG
                     TVVAATSEPGRVVTNGMSQYSRNERNANSGLVVSVTPDDYPDHVLAGIDFQRELEARA
                     FELGGGNYDAPGQLIGDFLAGRPSKEFGSVQPSYKPGVRLTDLSAALPGYAIEAIREA
                     LPAFDRQIRGYAKADGVLTGVETRTSSPIRIRRGDDFQSLNTRGLFPAGEGAGYAGGI
                     LSAGVDGIKIAEAVALDIVGQRAHGGEQLGQQTY"
     gene            complement(1725..2198)
                     /locus_tag="VM406_09000"
     CDS             complement(1725..2198)
                     /locus_tag="VM406_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HVL76142.1"
                     /translation="METLTIGLISDTHGVLRPEARVALRGCAHILHAGDICDPAVLRQ
                     LEEIAPVTAVRGNNDRGTWAEALPAHAVVEFAGVRVCVVHDLADYRVPAPDEARHDVV
                     VTGHSHRPAIRMEGGVLYVNPGSAGPRRFTLPISLGFLEIAGGRAKARLQTLEVG"
     gene            complement(2249..3043)
                     /gene="ygiD"
                     /locus_tag="VM406_09005"
     CDS             complement(2249..3043)
                     /gene="ygiD"
                     /locus_tag="VM406_09005"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HVL76143.1"
                     /translation="MNDTRTNRMPAVFVGHGSPMNALEDNAWTRGWESLGARIGKPAA
                     ILAVSAHWLTHGTAVTAMEAPPTIHDFGGFPQALFDIQYPAPGSPALAERVRDLLAPL
                     EVMLDDEWGLDHGTWSVLHKMFPQADIPVLQLSMDADQPPSFHLALGERLRPLRDEGV
                     LILATGNVVHNLRTIRWNSKEGYDWAERFNTRIRGLLERGDVAALADTSQWDDDARRA
                     VPTIEHYLPLLYVAGARREDEPFEIVIDGLEMGSISMLSVAAGLPR"
     gene            complement(3096..3512)
                     /locus_tag="VM406_09010"
     CDS             complement(3096..3512)
                     /locus_tag="VM406_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006484043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HVL76144.1"
                     /translation="MNHSASDYAATLLRITLGVVLLAHGLLKVLVFTLPGTAGFFASV
                     GFPGWTAYPVTAIEIAGGLALIAGFHSRLVALAVQPVLLGALFVHIGNGWLFSAPNGG
                     WEYPLFLVVAAAVVSLLGDGAFALRRAPAPVRLRTA"
     gene            3667..4605
                     /locus_tag="VM406_09015"
     CDS             3667..4605
                     /locus_tag="VM406_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL76145.1"
                     /translation="MKVFVKVAESGSLSAAARALDMSNPSVTRHVADLEAYLHARLFN
                     RSTRRLSLTETGAAYLERCRQLLQDLEDAALAASMSAARPAGTLRVNAPVSFAVNYLA
                     RALPEYAARYPEVKLDLTLSDRVVDLIEEGYDVAIRISRMPDSSALVARQIASARNLV
                     CAAPSYLKRCGAPSTPEELEQHACLTYTYLASRDEWQFMRDGKLHVARVRGPLVANNG
                     DLLREAAIAGMGIIVKPSFIIGDAIRQKRLVPLLTDYTMPDLPIFAVYPSRRHLSAKV
                     RTFVDFLVERYREHPDWDRDLDESLSKRSTPRKGKP"
     gene            complement(4744..6141)
                     /locus_tag="VM406_09020"
     CDS             complement(4744..6141)
                     /locus_tag="VM406_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76146.1"
                     /translation="MTSASDTPSTSPRTAAAGVVLIMLVVLARAFGGPGWLFIGLMAG
                     AALLVWAGFSTIPARLRNTAFVLFGLTILLLPVARAPLEAIQRGVFISGQLLALIASV
                     LLLAHCARSSRHIQRVGAALRGQPLSRRYLSFSITGQLFSGMLGMAGAHIMLVMAAPA
                     QEKADPTRVESVVGVTRAFSVAGFWSPVFGNMVILLALYPTLDWIDIFPVGVVLAQMG
                     VVVGYLLHRREARRASSGTPGDAEGRHGVADLLVDGLPLLIAMLAFMGVILTASHLIG
                     IVVPATIILLGPLFALLIHLALAAPGRRLVQALSNLRDSVLQYPRLASEALLFMAAGC
                     AGSIMADAFPPSWAQAIGAVLGGHPFPAIAFLMISIMAASLAGIHAVLSAVFLASTIT
                     PQVLGLPPLTHMAAILVGWGLAAVVAPFSVLSLTAARYAGTSIYDISFGRNWLFTLMT
                     TLLACAALSIITNML"
     gene            complement(6229..6450)
                     /locus_tag="VM406_09025"
     CDS             complement(6229..6450)
                     /locus_tag="VM406_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3717 domain-containing protein"
                     /protein_id="HVL76147.1"
                     /translation="MELTLADLEDAINYWRALRPSTGEERALSPEVNALATLYALMIY
                     EQTRSVPLSGLDPVQAQLIEAWRGRPGRD"
     gene            complement(6460..7626)
                     /locus_tag="VM406_09030"
     CDS             complement(6460..7626)
                     /locus_tag="VM406_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HVL76148.1"
                     /translation="MSIAIAPLARPIVQNLGASRIREVANAGIGLPDVLPFWFGEPDQ
                     ETPDFIRAAAKEALDAGDTFYTHNLGIAPLREAIASYLSALHQPIDAGRITVTSSGVS
                     ALMLVAQLIINPGDRVVAVTPLWPNLVEIPKILGAEVEQVPLQFGQTWELDVQRLLEA
                     LTPGTRAVLLNSPNNPTGWVITAEQQEIILAHCRKHGIWILADDVYERLVYDGARCAP
                     SFLDLAEPQDRIVSANSFSKSWLMTGWRLGWIVAPQPMMADLGKLIEYNTSCAPGFVQ
                     RAGIVAVERGNEIIAHTVERYRAARDYLYQRLNAMPGVTTPLPKGAMYLFFRVDGERD
                     ALALCKRLVVEAKLGLAPGSAFGPEGEGYVRWGFASSLERLGEGADRLERFLRA"
     gene            complement(7687..7812)
                     /locus_tag="VM406_09035"
     CDS             complement(7687..7812)
                     /locus_tag="VM406_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76149.1"
                     /translation="MKHLKQAALGTAFAAVLALAFASYLQPAFVLDLANRIVLCF"
     gene            complement(7829..9046)
                     /locus_tag="VM406_09040"
     CDS             complement(7829..9046)
                     /locus_tag="VM406_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL76150.1"
                     /translation="MLTASRAPDLKTVLIASGLVLTLAMGVRHGFGFWLQPISQAHGW
                     TRETFSLAMALQNLLWGLFGPFAGMAADRFGTARVVIFGALCYAGGLVWMASVSHPTA
                     FVVGSGILIGMALACTAFGAVAGIIGRTAPEAKRSWAFGISSAASSFGQFAMMPIEQQ
                     LISAAGWQNAFYILAALVAIVMLPMAFGLREQPVDKHAGPQQSIMEATREAFAYRPFQ
                     LLVAGYFVCGFQVVFIGVHMPAYLKDKDIADPSVAVMALALIGLFNIFGSYYAGKLGG
                     FLPKRYLLSSIYFTRAIVIALFLLAPLSAFSVYVFAAAMGLLWLSTVPLTNGVIAGIF
                     GVKYMAMLSGFVFFSHQVGSFLGVWLGGYLFTRDGNYNTVWMITIALGVFAALVNLPI
                     NEKPIARSQAALA"
     gene            complement(9278..10114)
                     /locus_tag="VM406_09045"
     CDS             complement(9278..10114)
                     /locus_tag="VM406_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008168960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL76151.1"
                     /translation="MKPASHYVRVGEHEIHYVEWGTPGAPVIVMWHGLARTGRDFDDL
                     AEALADSYHIVCPDTIGRGLSQWSTKPDEQYRLDFYAQLAQGFVDALGFGQLRWVGTS
                     MGGAIGTVAAATTLKGRISHLVLNDIGPALNPVAIERIRSYAGNPPQFDTVTELETFF
                     RTVYKPYGWLSDAQWRRMTETSTRRLPNGKVTPHYDPNMVRQFIVHPHDYDRWADYDA
                     LDMPVLLLRGVDSDLLLQETAEEMTRRGPRARLEVIQGCGHAPALNVPEQIALVRNFL
                     MR"
     gene            complement(10111..11175)
                     /locus_tag="VM406_09050"
     CDS             complement(10111..11175)
                     /locus_tag="VM406_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVL76152.1"
                     /translation="MITRILSHDLPRNRVLTLIMLAILFGLAFAPFLFPGAKALNVAA
                     KILIFIVLVASYDLLLGYTGIVSFAHTMFFGIGAYGIAIASAKMGASWSSLAIGALGA
                     VALSLVLSLIIGLFSLRVRAIFFAMITLAVASAFMTLASQMSHITGGEDGLNFRVPEL
                     LTPAFMMAEEKFMGAWLDGRLITYYLLFFAALGLFLAMLRIVNSPFGRVLQAIRENDF
                     RAEAIGYKTVIYRTMSNVLAAVFATLAGCMLAIWLRYNGPDTSLSFEIMIDILLIVVI
                     GGMGTMYGAVCGTVLFLLAQNYLQDLMRLGSEATQNIAIVSALLSPDRWLLWLGILFI
                     LSVYFFPTGVVGKLRAWSAR"
     gene            complement(11184..>11954)
                     /locus_tag="VM406_09055"
     CDS             complement(11184..>11954)
                     /locus_tag="VM406_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVL76153.1"
                     /translation="LGAFVAVSVLTRMPGLMGSDSFAMNLAAIGAAMLAAMIVAGAIG
                     LFFERVIVSRVYGQHLKQILITMGGMVVGEELIKVFWGPQQIALNLPASLRGSILIGE
                     SAVEKYRLLAVVVGLIVFAAMMLVLNRTKLGLLIRAGVQDREMVESLGYRIKRLFIGV
                     FVVGSALAGLGGVMWGLYQQSVYPHMGAQVNVLIFIVIIIGGLGSTGGCFLGALLVGL
                     LANYTGFLAPKVALFSNILLMAAILLWRPQGLYQVTNR"
BASE COUNT         2242 a         3905 c         4013 g         1794 t
ORIGIN      
        1 accaacgtac aactcccgct cgaccatccc cccgccgatc tgcaggcagc gatactcgca
       61 cgcctgcaga tcgccccgga gcaattgctg aaggtgaacg tgttccggcg cggctacgac
      121 gcgcgcaggc gcagcgcgat cacgctggtc tacacgctgg atgtcgaggt ggaggacgag
      181 cagggcgtgc tcgcgcgtct ggccggcgtg ccgggcgttg gcatcgcacc ggacacgagc
      241 tacaagttcg tcgcgcgggc accggccgcg ctgcgcaagc gcccggtcgt gatcggcttc
      301 ggtccctgcg gtatctttgc cgcgctgatt ctcgcgcaaa tgggtttccg cccgatcatc
      361 ctggagcgcg gcaaggtggt gcgcgaacgg acgaaggaca cctggggcct gtggcgccgg
      421 cgcgagctga accccgaatc gaatgtgcag ttcggcgagg gcggcgcggg caccttctcc
      481 gacggcaagc tgcacagcgg catcaaggac ccgaagcact acggccgcaa ggtgctgacc
      541 gagttcgtga acgcgtatgc gccggaagag atcctgtacg tgtccaagcc gcacatcggc
      601 actttccgcc tggtgaagat ggtggaggcg atgcgcgcga caatcgaatc gctgggcggc
      661 gagttccgct tcgaggccaa ggtcagcgac atcgcgatcg agaacggccg ggtgcgcggc
      721 gtgcagatcg agaacggcga cttcatcgag gccgatcacg tggtgctggc ggtgggccac
      781 agcgcgcgcg acaccttcca gatgctgcat gagcgcggcg tgtacatcga agccaagccg
      841 ttctcgatcg gctttcggat cgagcacccg cagtcgatga tcgaccgcgc gcgcttcggc
      901 aaattcgccg ggcatccgct cttgggcgcg gccgactaca agctcgtgca ccatgcgaag
      961 aacgggcgcg cggtctacag cttctgcatg tgcccgggcg ggaccgtggt ggcggccaca
     1021 tccgagccgg ggcgcgtcgt caccaacggc atgagccagt actcgcgcaa cgagcgcaat
     1081 gccaacagcg ggctggtggt cagcgtcacg ccggacgact atcccgacca tgtgctggcc
     1141 ggcatcgatt tccagcgcga gctggaagcg cgcgcgttcg aactcggcgg cggcaactac
     1201 gacgcgcccg gccagctgat cggcgatttt ctcgccgggc ggccgtcgaa agaattcggc
     1261 tcggtccagc catcgtacaa gccgggcgtg cggctcaccg atctgtcggc tgcgctgccc
     1321 ggctacgcga tcgaggcgat ccgtgaagcg ctgcccgcgt tcgaccgcca gatccgcggc
     1381 tatgcgaagg ctgatggcgt gctgaccggc gtggagacgc gcacttcatc gccgatccgg
     1441 atccgccgcg gcgacgattt ccagagcctg aatacgcgcg ggctgtttcc ggccggcgag
     1501 ggggccgggt atgcgggcgg gattttgtcg gccggtgtcg acggcatcaa gattgcggag
     1561 gcggttgcgc tggatatcgt cggccagcgc gcccatggcg gcgaacagtt ggggcagcag
     1621 acgtattagc gcaggggcgg ccgcgtccca cgggggtagg ctgggatgca atttcagctt
     1681 gtggcagggc ggtcggtcga actttgctgg gcttgcatcc cagcctaccc cacctccagc
     1741 gtctgcaacc gcgccttcgc ccgcccgccg gcaatctcca gaaaccccag cgaaatcggc
     1801 agcgtgaagc gacgcggccc tgcgctgccg gggttcacat acagcacgcc cccctccatg
     1861 cggatcgccg gccggtgcga atgcccggtc accaccacgt catggcgcgc ctcatctggt
     1921 gcgggcacgc gataatcggc cagatcgtgc accacgcaca cgcgcacgcc agcgaactcc
     1981 accactgcgt gcgccggcaa ggcttcggcc caggtgccgc gatcattgtt gccgcgcacc
     2041 gccgtcaccg gcgcaatttc ctccagctgc ctgagcaccg cgggatcgca gatgtcgccc
     2101 gcgtgcagga tgtgcgcgca gccgcgcagc gcgacgcgcg cttctgggcg cagcaccccg
     2161 tgcgtatcgg aaatgagacc tatcgtaagc gtttccatgt ccggcagtat tgcaaatgcc
     2221 gaacgtgatg caagaagcgg gcgagccgct acctgggcag ccccgccgcc acgctcagca
     2281 tgctgatcga acccatttcc agcccgtcga tcacgatctc gaacggctcg tcctcgcgcc
     2341 gcgcgccggc aacatagagc agcggcagat agtgttcgat cgtcggtacc gcgcggcgcg
     2401 catcgtcgtc ccactgcgag gtgtcggcca gcgccgcgac gtcgccgcgc tcgagcaggc
     2461 cgcggatgcg tgtgttgaag cgctcggccc agtcataccc ctccttgctg ttccagcgta
     2521 tcgtgcgcag gttgtgcacg acgttgccgg tggcgaggat cagcacgcct tcgtcgcgca
     2581 gcgggcgcag gcgttcgccg agtgcaagat gaaacgacgg cggctgatcc gcgtccatgc
     2641 tcagttgcag cacggggatg tcggcctgcg gaaacatctt gtgcagcacc gaccaggtgc
     2701 cgtggtccag tccccactcg tcgtcgagca tcacctcgag cggggcgagc aggtcgcgca
     2761 cgcgctcggc aagcgccggg ctgccggggg cagggtactg gatgtcgaac agcgcctgcg
     2821 ggaagccgcc gaaatcatgg atggtcggcg gcgcctccat tgcggtgacg gccgtgccgt
     2881 gcgtgagcca gtgcgcggag acggcgagga ttgcggcggg cttgccaatg cgcgcgccca
     2941 ggctttccca gccccgcgtc caggcattgt cttccagcgc attcatcggg ctgccgtggc
     3001 cgacgaagac ggcgggcatg cggttggtgc gggtgtcgtt catggcgaga atccttcagt
     3061 gagtgggcgc gggcggcgtg cgccgccctg ggagactacg cggtgcgcag gcgaaccggg
     3121 gccggcgcgc ggcgcagcgc aaaggcgccg tcgcccagca ggctcaccac ggcggctgcg
     3181 accaccagga acagaggata ttcccagccg ccgttcggcg cactgaacag ccagccgttg
     3241 ccgatgtgga cgaacagtgc accgagcagg accggctgca cggcgagcgc aaccaggcgc
     3301 gagtggaagc cggcgatcag cgcaaggccg cccgcgattt cgatcgcggt gaccgggtag
     3361 gcggtccagc ccgggaagcc tacgctggca aagaagccgg cggtgccggg cagcgtgaag
     3421 acgagcacct tgagcaggcc atgggcgagc agcacgacgc cgagcgtgat gcgcagcagg
     3481 gtggcggcgt agtcggaagc ggagtggttc attggatgtc ctttcagttc ggggttggca
     3541 ttgacgatgc gtggagcacc gttgtccaca ctgtagtacc ctccgcaatt tggaaaaata
     3601 cggcgtcagg aaattgatta tttcggatat cggaaatata gcgcggcaat ggacctcttc
     3661 cagtcaatga aagtcttcgt gaaggtggcg gaaagcggca gcctgtccgc cgccgcccgc
     3721 gcgctcgaca tgtccaatcc ctcggtcacc cgccatgtgg cagacctcga ggcctatctg
     3781 cacgcgcggc tgttcaaccg cagcacccgg cggctgtcgc tgaccgagac cggggcggct
     3841 tacctggagc gctgccggca gttgctgcag gatctggagg acgcggcact ggccgcgagc
     3901 atgagcgccg cgcgccccgc aggcacgctg cgggtgaacg cgcccgtctc gttcgccgtc
     3961 aactatctgg cgcgcgcact gcctgagtac gcggcgcgct atccggaagt gaaactcgac
     4021 ctgaccttgt cggaccgcgt ggtcgatctg atcgaggagg gctacgacgt ggcgatccgg
     4081 atctcgcgca tgcccgacag ctcggcgctg gtcgcgcgcc agatcgccag cgcgcgcaac
     4141 ctggtctgcg cggcgccgtc ctacctgaag cgttgcggcg cgccgtccac gccggaggaa
     4201 ctggaacagc atgcttgcct gacctacacc tacctggcgt cgcgcgacga atggcagttc
     4261 atgcgtgacg gcaagctgca cgtggcgcgc gtcaggggac cgctggtggc caacaatggt
     4321 gatctgctgc gcgaggccgc gatcgcgggc atgggcatca tcgtcaagcc ttcgttcatc
     4381 atcggcgacg cgatccgcca gaagcggctg gtgccgctcc tgaccgacta cacgatgccg
     4441 gacctgccga tcttcgccgt atatccgagc cgccgccacc tgtcggccaa ggtgcgcacc
     4501 ttcgtcgatt tcctggtgga gcgctaccgc gagcatccgg actgggaccg cgacctcgac
     4561 gagagtctct cgaaacgttc cacgcccagg aaaggaaagc catgaccgct ggcacgatcg
     4621 cgctcgacgg taccctgtcc agtacctgct cgagaagtcc atcaaccgca agctgaacga
     4681 cgacgcgcgc gccgacttgt ctatggcccc ggtacgagag gggctggtgc tggcgcgcaa
     4741 gcgctagagc atgttggtaa tgatggaaag cgccgcgcag gccagcagcg tggtcatcag
     4801 cgtaaacaac cagttgcgcc cgaagctgat gtcgtagatc gatgtgccgg catagcgcgc
     4861 cgctgtcagc gacagcacgg agaatggcgc caccacagcg gccagccccc agccgaccag
     4921 gatcgccgcc atgtgggtca gcggcggcag ccccagcacc tgcggcgtga tggtcgaggc
     4981 caggaacacc gccgacagca ccgcgtgaat gccggccagc gaagccgcca tgatgctgat
     5041 catcaggaag gcgatggcgg ggaagggatg gccgccgagg accgcgccga tcgcctgggc
     5101 ccatgaaggc ggaaacgcat ctgccatgat gctgccggca cagccggctg ccatgaacag
     5161 cagcgcctcg ctcgccaggc gcggatactg cagcacgctg tcgcgcaggt tggacagggc
     5221 ctgcaccagc cgtcgacccg gcgcggccag cgcgagatgg atcagcaacg caaacagggg
     5281 gccgagcagg atgatggtgg ccggcacgac gatgccgatc aggtggctcg ccgtgaggat
     5341 cacgcccatg aaagcgagca ttgcaatcag caggggcagg ccgtcgacga gcaggtccgc
     5401 tacgccgtga cgcccctctg cgtctcccgg cgtgccggaa gacgcgcggc gcgcttcgcg
     5461 ccgatgcagc agatagccga cgacgacgcc catctgcgcc agcaccacgc cgaccgggaa
     5521 gatgtcgatc cagtccagcg tcggatacag ggccagcagg atcaccatgt tgccgaacac
     5581 cggactccag aaacccgcca cgctgaacgc gcgggtcacg ccgaccacgc tctccacccg
     5641 cgtcgggtct gccttctcct gcgcgggagc ggccatcacc agcatgatgt gggcgccggc
     5701 catgccgagc atgccggaaa acagctggcc ggtgatcgag aacgaaaggt agcgccggct
     5761 aagcggctgt ccgcgcagcg ccgcgcccac gcgctggata tgccggctgc ttcgtgcgca
     5821 gtgcgccagc agcagcacgc tggcgatcag cgccagcaac tgccccgaga tgaacacgcc
     5881 gcgctggatc gcctccagcg gcgcgcgcgc gaccggcagc agcaggatcg tgaggccgaa
     5941 cagcacgaag gcggtattgc gcagccgcgc gggaatggtg gagaaccccg cccagaccag
     6001 cagcgcggcg cctgccatca acccgatgaa cagccacccg ggcccgccga acgcacgtgc
     6061 gagcacgacc agcatgatca gcacgacgcc ggccgcggcc gtcctgggtg aggtgctggg
     6121 ggtgtctgag gccgatgtca ctggggtggc gctaccggag gagggaaggc aagaaccgat
     6181 tatgcagcaa tcagccgttt ccctgttgcg gcgcggcaag aggcccggtc agtcccggcc
     6241 ggggcggccg cgccaggcct cgatcaattg cgcctgcacc ggatcgagac cggacagggg
     6301 caccgatctg gtttgctcgt agatcatcag cgcataaagc gtggcgagcg cattcacttc
     6361 cggcgacaag gcccgctctt ctccagtgga gggacgcagc gcgcgccagt agttgatcgc
     6421 gtcttcgagg tcggcgaggg tgagttccat gcagcgattc tacgcgcgca gaaaccgttc
     6481 cagccggtct gccccttcgc ccagcctctc cagcgaactc gcaaaacccc accgcacata
     6541 cccttcgcct tccggcccga acgcgctgcc cggtgcgagc cccagcttcg cctccaccac
     6601 cagccgcttg cacagcgcga gcgcgtcccg ttcgccgtca acgcggaaga acaggtacat
     6661 cgcgcccttc ggcagcggcg tggtcacgcc cggcatcgcg ttgagacgct gatagagata
     6721 gtcgcgcgcg gcgcggtagc gctcgacggt gtgcgcgatg atctcgttgc cgcgctcgac
     6781 tgcgacaatg ccggcgcgct gcacgaagcc cggcgcgcag gacgtgttgt actcgatgag
     6841 cttgccgagg tccgccatca tcggctgcgg cgcgacgatc cagcccaggc gccagccggt
     6901 catcagccag gacttggaaa agctgttggc cgagacgatg cggtcctggg gctcggccag
     6961 atcgagaaac gacggggcac agcgcgcgcc atcgtagacg aggcgttcgt agacgtcgtc
     7021 ggcgaggatc cagatgccgt gcttgcggca gtgggcgagg atgatttcct gctgctcggc
     7081 ggtgatcacc cagccggtcg gattgttcgg cgaattgagc aggaccgcgc gcgtgccggg
     7141 cgtgagtgct tccagcagcc gctgtacatc cagctcccag gtctggccga actgcagcgg
     7201 cacctgttcg acctcggcgc cgagaatctt cggaatctcc accaggttcg gccacagcgg
     7261 cgtcaccgcc accacgcggt cgcccgggtt gatgatcagc tgggccacca gcatcagcgc
     7321 ggaaacgccg gaggaggtga ccgtgatccg gccggcatcg atgggctggt ggagcgcgga
     7381 cagatagctg gcgattgctt cacgcagcgg cgcgatgccg aggttgtgcg tgtaaaaggt
     7441 gtcgccggca tcgagcgcct ccttggcggc ggcacggatg aaatccggcg tttcctggtc
     7501 gggttcgccg aaccagaacg gcagcacatc gggcaggccg atgccggcat tggcgacttc
     7561 acggatgcgc gatgcgccga ggttctggac gatggggcgg gcgagcggag cgatggcgat
     7621 ggacatgacg gcagaacgaa aggggcggca gaacgaaagg ggcggcagcc cttgcggcta
     7681 gccggatcag aagcagagga cgatgcggtt ggcgagatcg agcacgaagg ccggctgcag
     7741 atagctcgcg aaggcaagcg ccagcaccgc cgcgaaggcg gtgccgagag cggcttgctt
     7801 caagtgtttc atgctcggct ccgaaggctc aggccaacgc cgcttgcgag cgtgcaatcg
     7861 gcttttcatt gatcggcagg tttaccagcg cggcgaacac gcccagcgca atggtgatca
     7921 tccacacggt attgtagttc ccgtcgcgcg tgaacagata gccgcccagc cacacgccga
     7981 ggaagctgcc gacctggtgc gagaagaata cgaagccgga cagcatcgcc atgtacttca
     8041 cgccgaagat gcccgcgatc acgccattgg tcagcggcac ggtggacagc catagcagac
     8101 ccatcgcggc ggcgaacacg tataccgaga acgcgctaag gggtgcgagc agaaacagcg
     8161 cgatcacgat tgcgcgggtg aaataaattg acgagagcag gtagcgcttg ggcaggaagc
     8221 cgccaagctt gccggcgtag taggaaccga agatgttgaa caggccgatc agcgccagcg
     8281 ccatcaccgc cacgctcggg tccgcaatgt ccttgtcctt caggtaagcc ggcatgtgca
     8341 cgccgatgaa caccacctgg aagccgcaca cgaagtagcc cgccaccagc agctggaacg
     8401 gccgataggc gaaggcttcg cgcgtggctt ccatgatgct ttgctgcggg ccggcgtgct
     8461 tgtcaaccgg ctgctcgcgc aggccgaagg ccatcggcag catcacgatc gcaaccagcg
     8521 cggccaggat gtagaaggcg ttctgccagc cggcggcgga gatcagttgc tgctcgatcg
     8581 gcatcatggc gaactggccg aaggaactgg cggcactgga aatgccgaag gcccacgagc
     8641 gcttcgcctc gggcgcagtg cgcccgatga tgccggccac cgcaccgaag gcggtgcacg
     8701 cgagcgccat gccgatcagg atgccggagc cgaccacgaa ggcggtcgga tgcgagacgc
     8761 tcgccatcca gaccaggccg ccggcgtagc agagcgcgcc gaagatcacc acgcgcgcag
     8821 tgccgaagcg atccgccgcc atgccggcaa aggggccgaa caaaccccac agcaggttct
     8881 gcagcgccat cgccagtgaa aaggtctccc gggtccagcc atgcgcctgc gagatcggct
     8941 gcagccagaa gccgaagcca tgacgcacgc ccatcgcaag cgtcaggacc aggccgcttg
     9001 cgatcagcac ggttttcaga tcaggggcgc gactggcggt aagcatgacg ggcgggcgtt
     9061 gaaaagagag aaatcgagtg tagcgcaaag caaaaatgca tgtggaccgg ccggatcacc
     9121 gggccagcca tggcgcaaac acaatctcga acgaacggat gtccgcccgg ccggcggttc
     9181 cgggcaagtc gatccccggt gccgtcaatg cgatatatgg aacggaattg cgcagtcggg
     9241 ctccggctgc cgccagaaaa acccgggttt tccggcctca gcgcatgagg aagttgcgca
     9301 ccaacgcaat ctgctccggc acgttcagcg ccggcgcgtg tccgcatccc tggatcacct
     9361 ccagccgcgc gcgcggcccg cgccgcgtca tctcctctgc ggtttcctgc agcagcagat
     9421 ccgaatccac gccgcgcagc agcagtaccg gcatgtcgag tgcgtcatag tcggcccagc
     9481 ggtcgtagtc gtgcggatgc acgatgaact ggcgcaccat gttcggatcg tagtggggcg
     9541 tgaccttgcc gttcggcagg cgccgcgtcg acgtctccgt catgcgccgc cactgcgcgt
     9601 cgctcagcca gccatagggc ttgtagacgg tgcggaaaaa ggtttccaac tcggtcacgg
     9661 tgtcgaactg cggcgggttg ccggcatacg agcggatgcg ttcgatcgcc accggattga
     9721 gtgcggggcc gatgtcgttc agcaccaggt ggctgatgcg tcccttcaag gtcgtcgccg
     9781 cggcgaccgt gccgatcgcc ccgcccatcg acgtgccgac ccagcgtagc tgcccgaagc
     9841 cgagcgcatc gacgaagcct tgcgccagtt gcgcatagaa gtcgagccgg tattgctcgt
     9901 ccggcttcgt gctccattgc gacagcccgc gtcctatcgt gtccgggcag acgatgtgat
     9961 agctgtctgc aagcgcctcg gcgagatcgt cgaagtcgcg accggtgcgc gccaggccgt
    10021 gccacatcac gatgaccggc gcgcccggcg tgccccactc gacatagtgg atctcgtgtt
    10081 cgccgacgcg aacgtaatgc gatgcgggct tcatcgtgcg ctccatgctc tgagcttgcc
    10141 gaccacgccg gtcgggaaga agtagacgga caggatgaac aggataccca gccacagcag
    10201 ccagcggtcc ggcgacagca gggccgagac gatcgcgatg ttctgcgtcg cctccgaccc
    10261 cagtcgcatc aggtcctgca ggtagttctg cgcgagcagg aacagcacgg tcccgcacac
    10321 cgcgccatac atcgtgccca tgccgccgat gacgacgatc agcaggatgt cgatcatgat
    10381 ctcgaacgac agcgaggtgt cggggccgtt gtagcgcagc cagatcgcca gcatgcagcc
    10441 ggccagcgtg gcgaacaccg cggccagcac attggacatg gtacggtaga tcacggtctt
    10501 gtagccgatc gcctcggcgc ggaagtcgtt ctcgcggatc gcctgcagca cgcgtccgaa
    10561 cggcgagttc acgatgcgca gcatcgccag gaacagaccg agcgcggcga agaacagcag
    10621 gtaatacgtg atcaggcggc cgtccagcca cgcgcccatg aacttctctt cggccatcat
    10681 gaacgccggt gtgagcagtt ccggcacgcg gaagttcaga ccgtcctcgc cgccggtgat
    10741 gtgcgacatc tgcgaggcca gcgtcatgaa cgctgaagcc actgccagcg tgatcatcgc
    10801 aaagaagatc gcccgcacgc gcagcgagaa caggccgatg atcagcgaca gcaccagcga
    10861 caacgcgacc gcgccgagcg cgccgatcgc caggctgctc cagctcgcgc ccattttcgc
    10921 gctggcgatc gcgatgccgt aggcgccgat gccgaagaac atcgtgtgcg caaacgagac
    10981 gatgccggtg tagccgagca gcaggtcgta gctggcgacc agcacgatga agatcaggat
    11041 cttcgccgcc acgttcagcg ccttcgcgcc cgggaacagg aagggtgcga atgcgagtcc
    11101 gaacaggatc gccagcatga tgagggtcag cacgcggttg cgcggcaggt catgggacag
    11161 gattcgggtg atcatggcgg gcctcagcga ttcgtgacct ggtacagtcc ctgcgggcgc
    11221 cacagcagga tggcagccat cagcaggatg ttggagaaca gcgcaacttt cggcgcgaga
    11281 aagcccgtgt agttggccag caggcccacc agcagcgcac cgaggaagca gccgccggtc
    11341 gagccgaggc cgccgatgat gatcacgatg aagatcagca cgttgacctg cgcgcccatg
    11401 tgcgggtaga cgctctgctg gtacagaccc cacatcaccc caccgaggcc ggccagcgcg
    11461 gagccgacga cgaacacgcc gatgaacagc cgcttgattc ggtatcccag actttccacc
    11521 atctcgcggt cctgcacgcc ggcgcggatc aacaggccca gcttggtgcg gttcaggacc
    11581 agcatcatcg ccgcgaacac gatcaggccg acgacgaccg cgagcaggcg gtacttctcc
    11641 accgccgatt cgccgatcag gatcgagccg cgcagcgaag cgggcaggtt cagcgcgatc
    11701 tgctgcgggc cccagaacac cttgatcagc tcttcgccga ccaccatgcc gcccatcgtg
    11761 atcaggatct gcttcaggtg ctggccgtag acgcggctga cgatcacgcg ctcgaagaac
    11821 aggccgatcg cgccggcgac gatcatcgcg gccagcatcg ccgcgccgat cgcggcaagg
    11881 ttcatcgcaa agctgtccga gcccatcaga cccggcatgc gcgtgagcac cgataccgcg
    11941 acgaaggcgc caag
//
LOCUS       DATLGF010000297         5001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_6389,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000297 DATLGF010000000
VERSION     DATLGF010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6389"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..509)
                     /locus_tag="VM406_09060"
     CDS             complement(132..509)
                     /locus_tag="VM406_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76154.1"
                     /translation="MNTAERKQQLIDRGALYRARVMLERERTRASLRPLRLSADGVQK
                     LAATAITAYAGASAASALARHLPAILPPVLRLLASVGARKGPRQAAWRFAAIGAVGAA
                     MAAAVVWMRRLRRQTPDRVRRAE"
     gene            complement(509..895)
                     /locus_tag="VM406_09065"
     CDS             complement(509..895)
                     /locus_tag="VM406_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HVL76155.1"
                     /translation="MEQPHPPRERAGLVAGLSAVARNLFALFVTRAELAALELGEARD
                     HLAKLFLVGALGVLLVCFALAGWTALIVVLAWDALGWKILLLVAVVYTLLAGAALLAA
                     RSMLAQDKLGFPATMAELRNDREALF"
     gene            complement(909..1211)
                     /locus_tag="VM406_09070"
     CDS             complement(909..1211)
                     /locus_tag="VM406_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HVL76156.1"
                     /translation="MVTSNIKTVRNDMRTLVQDAQELFREASMATGEKAEELRARGME
                     LLEAAIEKTHELQAVALEKGKVAAKNADEYVHQNPWQAVAMSAGIGLLVGLLISRK"
     gene            complement(1335..1838)
                     /locus_tag="VM406_09075"
     CDS             complement(1335..1838)
                     /locus_tag="VM406_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HVL76157.1"
                     /translation="MKTRIALIGAAAIVAGCATRPDTPPPAEAPPEPEPPAVLAPTPR
                     PEPLPASPARSIDEYKRVLARHIAARSPARVFDGRPQALLRSVIVLRYTLDADGNLVQ
                     SEVMRSNRDRETEATALATLRAAAPFPRPADHLLHRGRIEISETWLFNNDGRFQLRTI
                     AERQMDR"
     gene            complement(1929..2912)
                     /gene="pip"
                     /locus_tag="VM406_09080"
     CDS             complement(1929..2912)
                     /gene="pip"
                     /locus_tag="VM406_09080"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HVL76158.1"
                     /translation="MTSAADAILFPPIEPRRHGMLAVDAIHTLYWEESGNPDGIPVVV
                     LHGGPGAGTSPRHRRYFDPARYRIVLFDQRGAGKSTPLGEWRDNTTPLLVADIERLRI
                     ELGIDRWLVFGGSWGSTLALAYGQAHPERCLGFVLRGIFLCTPQEIDWFLHGMRWFFP
                     EEHAAFVAPISHDERGELLQAYARRLFDDDPAVYVPAARAWSRYEGACLFLRPEPEAI
                     ADFSSDTVSLGVGRLEAHYFLHQGFFEQDQLLRGVDRIAHLPCMIVQGRYDVVCPPAT
                     AFRLRDAWPGAGFELIGDAGHSGYEPGIARALVAATERFKAVGHFGSVDAL"
     gene            3026..3102
                     /locus_tag="VM406_09085"
     tRNA            3026..3102
                     /locus_tag="VM406_09085"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3060..3062,aa:Asp,seq:gtc)
     gene            3242..3715
                     /locus_tag="VM406_09090"
     CDS             3242..3715
                     /locus_tag="VM406_09090"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HVL76159.1"
                     /translation="MDLPAHQLSITVLMTPDTANFSGKVHGGTILKLLDQAAYACASR
                     YAGRYVVTLSVDQVIFRQPIHVGELVTLLASVNHTGTSSMEVGIKVVAENIRTQETRH
                     VNSCFFTMVAVDDDGKPTQVPPLRPFTPDERRRYAAAELRKTMRREMEQRFASIK"
     gene            complement(4009..4920)
                     /locus_tag="VM406_09095"
     CDS             complement(4009..4920)
                     /locus_tag="VM406_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HVL76160.1"
                     /translation="MWFKNLQIYRLTAPWTLSAEQLEEKLAPLAFEPGSSVEMQAQGW
                     AAPRAKGGLVHSVNRQLLLQLVEEKKLLPSTVVNQVSKARAADLEEQQGFKPGRKQMK
                     EIKEQVTDELLPRAFSIQRSTRVWIDPVNGWLVVDASGAAKADDVFKMLLKSLEVLPF
                     AALRTERSPLSAMTDWLAADEAPAGFTVDQDTELRATGEGKATVRYVRHTLEPDDVRR
                     HIAAGKQCTRLALTWADRVSFVLTETLAIKRVAPLDVLKENQDAALMNEDERFDNDFA
                     LMTGELAKLIADLVEALGGEEKQKQAA"
BASE COUNT          823 a         1629 c         1706 g          843 t
ORIGIN      
        1 gccggacagc acaacggccg cagccagcgc ggaaagcgag agttgcttgc gcatgttgat
       61 tcctcgttgt tgtaaaacga gaattcgacc cgcgcgcggg ctgccgagtt cgaagagtta
      121 caaaaggaaa actattcggc ccgccgaacc cggtcgggcg tttgcctgcg caggcgccgc
      181 atccacacaa cggcggccgc catggcggct ccgacggctc cgatggctgc gaagcgccat
      241 gccgcctggc gcgggccctt gcgcgccccc acggaggcca gcagacgcaa tacgggtgga
      301 agaatggccg gcaggtgccg cgcgagcgcg gaggcggcac tggcgccggc ataggcggtg
      361 atggcggtgg cggcgagctt ctgcacgccg tctgccgaca gccgcaacgg gcgcaggctc
      421 gcgcgcgtgc gttcgcgttc cagcatcacc cgtgcacggt agagcgcgcc ccggtcaatc
      481 agctgctgct tgcgctcggc ggtattcatc agaacagggc ttcgcggtcg ttgcgcagct
      541 cggccatggt cgcggggaag ccgagcttgt cctgggcgag catcgaacgc gccgccagca
      601 gcgcggcgcc ggccagcagc gtgtagacca ccgccaccag cagcaggatc ttccagccga
      661 gcgcatccca ggccagcacg acgatcagcg ccgtccatcc ggccagcgcg aaacagacca
      721 ggagcacgcc gagcgctccg accagaaaca gcttggcaag gtggtcgcgc gcttccccga
      781 gttcgagcgc cgccagttcg gcgcgggtga cgaacagcgc aaacaggttg cgcgcgacgg
      841 cggacaggcc ggcgaccagc ccggcccgtt cgcgtggcgg atgcggttgc tccatgcgag
      901 cgcgcgcctt acttacgcga gatcagcagg ccgaccagca ggccgatgcc ggccgacatc
      961 gcgaccgcct gccacgggtt ctggtgcacg tattcatcgg cgttctttgc ggcgaccttg
     1021 cctttttcga gggcgacggc ctgcagttca tgcgtcttct cgattgccgc ttccagcagc
     1081 tccatgccgc gggcgcgcag ttcctcggcc ttctcgccgg tggccatgct cgcttcccgg
     1141 aacagctcct gggcgtcctg gaccagcgtt ctcatgtcgt tgcgtacggt cttgatgttc
     1201 gacgtaacca cggtaaaggc tcctttcagg gggatgactg cggcatgccg cacgcttggc
     1261 atgcggcagc ccagacttta ccaagttttg tcgtgcggaa gtttcgatcc gcgcaataag
     1321 tgcgtcgggc ggattcagcg atccatctgg cgttccgcga tggtgcgcag ctggaagcgt
     1381 ccgtcgttgt tgaacagcca ggtctcggag atctcgatgc ggccgcggtg cagcaggtgg
     1441 tcagccgggc gcgggaacgg ggcggccgcg cgcagcgtgg ccagcgcggt ggcctcggtc
     1501 tcgcggtcgc gattggagcg catgacctcg ctttgcacga ggttgccatc cgcatcgagg
     1561 gtatagcgca gcacgatgac cgaacgcagc agcgcctgcg gacgcccatc gaacacgcgc
     1621 gccgggctcc gggccgcgat atgccgggcc agcacgcgct tgtactcgtc gatgctgcgc
     1681 gccggcgatg ccgggagcgg ctccggccgc ggcgtgggcg ccagtacggc gggcggctcg
     1741 ggttccgggg gtgcctccgc gggcggcggc gtatcggggc gggtggcgca accggcgacg
     1801 atggcggcgg cgccgatcag ggcgattctg gtcttcatgc gtgacactcc cggcgaggtg
     1861 gatggagatg cgaccagtgt ccagtatctg gagaaaaagt tgctttggag aagcaaaatt
     1921 accggcgctt acaaagcgtc gacagagccg aaatgcccga ctgccttgaa acgctcggtt
     1981 gccgcgacca gcgcccgtgc gatgccgggt tcgtatcccg aatgcccggc gtcgccaatc
     2041 agctcgaaac ccgcgccggg ccacgcgtcg cgcagacgga acgcggtcgc cggcggacac
     2101 acgacgtcgt agcggccctg cacgatcatg cagggcagat gggcgatgcg gtcgacgccg
     2161 cgcagcagct gatcctgttc gaagaagcct tggtgcagga agtaatgcgc ctcgagccga
     2221 ccgacgccca ggctcacggt atcggagctg aagtcggcga tcgcctccgg ttccggcctg
     2281 aggaacaggc aggcgccttc atagcggctc caggcgcgcg cggccggcac gtacacggcg
     2341 gggtcgtcgt cgaacagccg gcgcgcatag gcctgcagca gttcgccgcg ctcgtcatgc
     2401 gaaatcggcg caacgaaggc cgcgtgctct tccgggaaga accagcgcat cccgtggagg
     2461 aaccagtcga tctcttgcgg cgtgcacagg aagatcccgc gcaggacaaa gccgaggcag
     2521 cgttcgggat gcgcctgccc gtaggcgagc gccagcgtcg agccccatga gccgccgaac
     2581 acgagccagc ggtcgatgcc gagttcgatg cgcagccgct cgatgtcggc gaccaggagc
     2641 ggcgtggtgt tgtcgcgcca ctcgccaagc ggcgtggact tgccggcgcc gcgctggtcg
     2701 aacagcacga tgcggtagcg cgccgggtcg aaatagcggc ggtggcgcgg cgaggtgccg
     2761 gcgccggggc cgccgtgcag caccaccacc ggtatgccgt ccggattgcc gctctcctcc
     2821 cagtacagcg tgtggatggc gtcgacggcc agcatgccgt ggcggcgcgg ttcgatcggc
     2881 gggaacagga tggcgtcggc ggctgaggtc atgatcggat ggaaaaatgg atgcaggaga
     2941 ttgtagttcc ccctgcggaa aaattgctgt acaatgcgac cccttctgga cctcgcggtt
     3001 tttcaagccg caggcaacga attctggagt ggtagttcag ttggttagaa taccggcctg
     3061 tcacgccggg ggtcgcgggt tcgagccccg tccactccgc caaatcgaaa aagacgagct
     3121 tgcgagctcg tctttttttc gcctgtttgc gcgatgatcg ggcctccggc gaacgcgagt
     3181 ggtgcgcaat tgcgtgcgac gctgcatcat atggcaaccg tcgcccacac tctcccttcc
     3241 catggacctg cccgcacacc aactcagcat caccgtcctg atgacgcccg acaccgccaa
     3301 cttctccgga aaggtccatg gcggcacgat cctcaagctg ctcgatcagg cggcgtacgc
     3361 ttgcgcaagc cgctatgcgg gacgctatgt ggtcacgctg agcgtcgatc aggtgatctt
     3421 ccggcagccg atacacgtgg gtgaactcgt gacgctgctg gcgtcggtga atcacaccgg
     3481 gacctcttcg atggaggtcg ggatcaaggt cgtggccgag aacatccgca cacaggagac
     3541 gcgccacgtg aacagctgct tcttcacgat ggtggccgtc gacgacgacg gcaagccgac
     3601 gcaggtgccg ccgctgcgcc cgttcacgcc ggacgagcgc cggcgctatg cggccgccga
     3661 attgcgcaag acgatgcggc gcgaaatgga gcagcggttc gcgtcgatca agtagcgtag
     3721 gggtgtcgct gctttcatgc ggatgagatc cctcgcttcg ctcgggatta cagctgggcg
     3781 gtgattcgtc ggccggtggt gtgtcggtgg ggtgttgccc cgggtgtgtc gccgcggtgt
     3841 gtcgtcctga gccgcaggcg aaggacctca tccgcctgcg gggaggtgca ccctgtgcgg
     3901 gacgatgaga cccttcgcct gcggctcagg atgacaggtt gaggctcagg atgacagatc
     3961 tgcggttcgc aatggcaggc cggcggcgag gcgctcgccg cccgcagctc acgccgcctg
     4021 cttctgcttc tcctcgccgc ccagcgcctc gaccaggtcg gcgatcagct tcgccagctc
     4081 gccggtcatc agcgcgaagt cgttgtcgaa gcgctcgtcc tcgttcatca gcgcggcatc
     4141 ttggttttcc ttcagcacgt ccagcggcgc gacgcgcttg attgccagcg tctcggtcag
     4201 cacgaaggac acgcggtcgg cccaggtcag cgccaggcgc gtgcactgct tgcccgcggc
     4261 gatgtggcgg cgcacatcat ccggctccag cgtatggcgc acgtagcgca ccgtggcctt
     4321 gccttcgcct gttgcgcgca gttcggtgtc ctggtccacc gtgaaaccgg ccggcgcctc
     4381 gtcggcggcg agccagtccg tcatcgccga cagcggcgag cgctcggtac gcagcgccgc
     4441 gaacggcagc acctcgagcg acttcaacaa catcttgaac acgtcgtcgg ccttggcggc
     4501 gccggacgca tccaccacca gccagccgtt gaccggatcg atccacacgc gcgtgctgcg
     4561 ctggatcgag aacgcgcgcg gcagcagttc atcggtgacc tgctccttga tctccttcat
     4621 ctgcttgcgg ccgggcttga agccctgctg ttcctccagg tcggcggcgc gcgccttgct
     4681 cacctggttg accaccgtgg atggcagcag cttcttttcc tcgaccagct gcagcagcag
     4741 ctggcgattc acgctgtgca cgaggccgcc cttggcgcgc ggcgcagccc agccctgggc
     4801 ctgcatctcg acgctggagc cgggctcgaa ggccagcggc gcgagctttt cctcgagctg
     4861 ctcggcggag agcgtccagg gagcggtcag gcgatagatc tggagattct tgaaccacat
     4921 ggcaggcccc ctttcaaaaa aggcgccatt ctacccgctg gggcgcgcga atctgtgtga
     4981 tccggacaac gcgttacatg t
//
LOCUS       DATLGF010000298         5412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_45064,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000298 DATLGF010000000
VERSION     DATLGF010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45064"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VM406_09100"
     CDS             complement(<1..617)
                     /locus_tag="VM406_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HVL76161.1"
                     /translation="MNGADVLCDTLLANGVDVCFANPGTSEMHFVAALDRKPQMRCVL
                     GLFEGVVTGAADGYARMAGKPAATLLHLGPGLANGLANLHNARRAGSPVVNIVGEHAS
                     YHLKHDAPLTSDIEGLARPMSAWVGRAEHADTVAQAAAEAVRASQAGGGGVATLILPA
                     DAAWTEVQHAAPVQAELDKPGIVAQSRIEQVAVALSSGRSTVLLLG"
     gene            complement(681..2006)
                     /locus_tag="VM406_09105"
     CDS             complement(681..2006)
                     /locus_tag="VM406_09105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HVL76162.1"
                     /translation="MSLALTASQPAGAISWPASLWRAGVPAGEHYRRLETDATVEVAI
                     VGAGYTGLTCASSLAERGIECAILDAAQPGYGASGRNGGMAVTRYKSGWASMHAAYGH
                     DTAYKLYRLILAGVDLIEALTQRHGLDNHFSRQGHLTPASSARASQRLHDDVRLLNEV
                     FHDSTVRMMDAAEVAQACGTGDYLDGYFDPRAAGIHPYLHATGLARAVHAGGTPIYGD
                     SPVLAISREGASYLLHTPGGTLRARKLVYATNGYTDMFSIQRSLERRIVAVSSAVIAT
                     EPLPEALAQRILPDNVLVTDTRHLVNYFRKTPGGHLLFGGRGSLTGVEKPQTYHGLER
                     RLVETFPELADHPIAFRWSGKVAVTLDDFPHIGAIGENAWFALGYGGRGVVLSHVLGA
                     ALADCVTGAPASLGPMSDNAFTEIPFHRFQVPGMNIVASYYKLKDRLGV"
     gene            complement(2006..2974)
                     /locus_tag="VM406_09110"
     CDS             complement(2006..2974)
                     /locus_tag="VM406_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVL76163.1"
                     /translation="MKPVRGILGCMIAAATLAAFTPAHAQNNYPSRAIKIVVPYQAGG
                     STDMVVRKLAELASPKLGQPVIIENKGGAGATLGARTIANAPPDGYTLAILPSPVFRM
                     PHMADTGYDPTKDFTYISMLSGYTLGVAVPATSPFKSWQDLVAHAKQNPGKISYGTAS
                     IGSASNVMMEEIAGRNGISWMHVPYKGESAVLVDVLGNRVDLYAGSTTVAPQVAAGGM
                     RMLVIWGENRSPLYPGTPTLKELDGTPPMNAPFGIAGPKGLPQPILTKLQTVFKQIVE
                     SPEFAKILTEYGQEPAYMDSATYTEYAARAFEQEREIVKKLGLAGS"
     gene            complement(3048..4106)
                     /locus_tag="VM406_09115"
     CDS             complement(3048..4106)
                     /locus_tag="VM406_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HVL76164.1"
                     /translation="MSRAEELHRKASIVDGLVFFSDGSTRDMKAGGVNALNLTVTDMA
                     SGYEQALKDVMAWRERCADPASGWMLIEQAEDIERAKAAGKVGLVMGWQNSRPLEDKI
                     ERIGIFHALGMRVMQLTYNEANLIGDGCLEKRNAGLSKFGFEAVREMNRVGVAIDISH
                     CGEQTCLDAAAASDKPVLLTHANANAVVARPRNKSDAVIKAVCDTGGVIGCSIHAYLL
                     WRGIAGQRPQLDDFVANVKYIGERYGYEHVGIGTDFPAVDTYEAVRHVMVMSRTKYPG
                     SAGDFSGQFGDVMEARFPVEVDTPAKLPALTQALEKGGLSEEQILGVLGGNFRRAFDA
                     IWGVAREDRREGAVRQAA"
     gene            4226..4990
                     /locus_tag="VM406_09120"
     CDS             4226..4990
                     /locus_tag="VM406_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HVL76165.1"
                     /translation="MEDSPLFISALAKGLCVLEAFNSSSPTMNLPEMAAACGISTSAA
                     QRIAFTLQQLGYLRKDERSKRYGLTVRAMSLGYAYLMTEPLLQRAHPVLNELGQECGE
                     SVNLSVPDGKDMVFVTRIPSKKHIPVYMPIGKRIPAISSASGRAVLSCRASDEVERFI
                     DSSPIERFTSRTTVDREQLLALVAEAAANGYAYAAEEYYLGDLNVAAAVVDHAGIPLA
                     AVNVSVPTSRWTLEQVKKDLGPLVIRAARAIGQRAD"
     gene            complement(5053..>5412)
                     /locus_tag="VM406_09125"
     CDS             complement(5053..>5412)
                     /locus_tag="VM406_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76166.1"
                     /translation="GGTVALPIWTDYMRVALRGVGPVHRAAPGGLAQVDGDLMYAEYA
                     AAGNSVRSVGIDEIRSFWERLFRWPSQPSQPSQPAPSVAPAPIVVPPAAMPTPPVQRP
                     VTPRPVEERRLEEWYRG"
BASE COUNT          873 a         1823 c         1774 g          942 t
ORIGIN      
        1 cccagcagca gcaccgtgga acgcccgctc gacaacgcga ccgcgacctg ctcgatgcgt
       61 gattgcgcaa cgatgccggg tttgtccagt tccgcctgga ccggggcggc atgctgcacc
      121 tcggtccacg ccgcatcggc cggcaggatc agcgtggcga cgccgccccc tccggcttgc
      181 gaagcccgca ccgcttcggc ggccgcctgc gccaccgtat ccgcatgctc ggcgcgcccg
      241 acccatgccg acattggcct agccagtcct tcaatgtccg aggtcagcgg cgcatcgtgc
      301 ttcaggtggt agcttgcgtg ctcgccgacg atattcacta ccggcgaacc ggcgcggcgc
      361 gcgttgtgga gattggcaag tccgttcgcg agccccggcc ccaggtgcaa cagcgtcgcc
      421 gccggttttc cggccatgcg cgcatagccg tcggcggcgc cggtgacgac gccctcgaac
      481 aggcccagca cgcagcgcat ctgcggcttg cggtcgaggg cggccacgaa gtgcatctcg
      541 gaagtgccgg gattggcgaa acagacgtcc acgccgttgg cgagcagcgt gtcgcacagc
      601 acatcggcac cgttcatcgt tgactccttc ggaaaatgag aaggcgcccg gaccgccgcc
      661 gggcgcctgg gtccgccgcg tcagaccccg aggcggtcct tgagcttgta gtagctggcg
      721 acaatgttca ttccgggcac ctggaaacgg tggaacggaa tttcggtgaa tgcattgtcg
      781 ctcatcggtc ccaggcttgc cggtgcaccc gtcacgcagt cggccagcgc ggcgccgagc
      841 acgtggctca gcaccacgcc gcgcccgccg tagccaagcg cgaaccaggc gttctcgccg
      901 attgcgccga tgtgcgggaa gtcgtcgagg gtgacggcca ccttgccgga ccagcggaac
      961 gcgatcggat gatcagccag ttccggaaag gtttcgacca gccggcgttc cagcccgtgg
     1021 taggtctggg gcttttcgac gccggtcagg ctgccgcgcc cgccgaacag caggtggccg
     1081 ccgggggtct tgcggaaata gttgaccagg tggcgcgtat cggtcaccag cacgttgtcg
     1141 ggcaggatcc gttgcgccag ggcttcaggc aagggctcgg tggcaatcac cgcgctgctc
     1201 accgccacga tgcgtcgctc caggctgcgc tggatggaaa acatgtcggt gtagccgttg
     1261 gtcgcgtaga ccagcttgcg cgcacgcagc gttcctcccg gcgtatgcag cagataactc
     1321 gcgccctcgc ggctgattgc gagcaccggg ctgtcgccgt aaatcggggt gccgcctgcg
     1381 tgcacggcgc gcgccagccc ggttgcgtgc agatacggat ggatgccggc cgcgcgcggg
     1441 tcgaaatacc catccagata gtcgccggtg ccgcaggcct gcgcgacctc ggcggcgtcc
     1501 atcatgcgca ccgtgctgtc gtggaatacc tcgttgagca gacgcacgtc atcgtgcagc
     1561 cgctgcgacg cgcgcgcgct cgatgccggt gtcaggtgtc cctgacggga gaaatggttg
     1621 tcgagaccat ggcgctgcgt gagcgcctcg atcaggtcca ctccggccag gatcagccgg
     1681 tacagcttgt aggcggtgtc gtgcccgtag gccgcgtgca tggacgccca cccggacttg
     1741 tagcgcgtca ccgccatgcc gccgttgcgc ccgctcgccc cgtagccggg ctgggccgcg
     1801 tcgaggatcg cgcactcgat gccgcgctcg gcgagcgacg aggcgcaggt caggccggta
     1861 tagccggcgc cgacgatcgc gacctcgacg gtcgcgtccg tttccaggcg ccggtaatgc
     1921 tcgcctgccg gcacgccggc acgccacagc gaggcgggcc agctgattgc gccggcaggc
     1981 tggcttgcag tcaatgcgag gctcattacg atccggccag tcccagcttc ttcacgatct
     2041 cgcgctcctg ctcgaaggcg cgcgccgcgt actcggtata ggtggccgaa tccatgtagg
     2101 ccggctcctg accgtattcg gtaaggatct tcgcgaactc gggcgactcg acgatctgct
     2161 tgaacacggt ctgcagcttg gtcaggatcg gctggggcag ccccttcgga ccggcgatgc
     2221 cgaacggcgc attcatcggg ggcgtgccgt cgagctcctt cagcgtcggg gtacccggat
     2281 acagcgggct gcggttctcg ccccagatca ccagcatgcg catgccgccg gcggccacct
     2341 gcggcgccac cgtggtcgag cccgcataca ggtccacccg gttgcccagc acgtcgacca
     2401 gtaccgcgga ctcgcccttg taaggcacgt gcatccagct gatgccgttg cggccggcga
     2461 tctcttccat catcacgttc gatgcgctgc cgatgctcgc cgtcccgtag ctgatctttc
     2521 cgggattctg cttcgcatgc gcgaccaggt cctgccagct cttgaacggc gaagtcgccg
     2581 gtaccgcgac accaagcgta tagccgctga gcatcgagat gtaggtgaag tccttggtgg
     2641 ggtcatagcc ggtatcggcc atgtgcggca tgcggaagac cggactcggc aggatcgcca
     2701 gcgtgtagcc gtccggaggc gcgttggcga tggtacgagc tcccagtgtc gcgccggcgc
     2761 cgcccttgtt ttcgatgatg accggttgcc ccagcttcgg cgatgcgagt tcggccagct
     2821 tgcgcacgac catgtcggtg gagccgcccg cctggtaggg gacgacgatc ttgatcgcgc
     2881 ggcttggata gttgttctgc gcgtgggccg gggtgaatgc tgcaagagtg gcggccgcga
     2941 tcatgcagcc gagaattccg cgtacgggtt tcatgtactc tccttcaaga caagactggc
     3001 cggcctgctg gccgggccgg tggttaccgc gccgaggcgc gggcgtttca tgcagcctgc
     3061 cgcaccgcgc cttcgcgccg gtcctcgcgg gccactcccc agattgcatc gaaggcgcgg
     3121 cggaaatttc cacctagtac gcccagaatc tgttcctcgc tcagtccgcc cttctccagc
     3181 gcctgcgtga gtgcaggcag cttcgcgggc gtgtccactt cgaccgggaa acgcgcctcc
     3241 atcacgtcgc cgaactggcc ggagaaatcg ccggccgatc ccgggtactt ggtgcgcgac
     3301 atcaccatca catggcgcac tgcctcatag gtatcgaccg ccggaaagtc ggtcccgatg
     3361 ccgacgtgct catagccgta gcgctcgccg atgtacttca cattggcgac gaaatcgtcg
     3421 agctgcggcc gctggccggc aatgccgcgc cagagcagat acgcgtggat cgagcagccg
     3481 atcacgccgc cggtgtcgca cacggccttg atgaccgcat cggacttgtt gcgcggacgc
     3541 gcaaccaccg cgttggcgtt cgcgtgggtg agcaggaccg gcttgtcgga agcggcggcg
     3601 gcgtcgaggc aggtctgctc gccgcagtgg gagatgtcga tcgcaacccc gacgcggttc
     3661 atctcgcgca ccgcttcgaa tccgaacttg gagagaccgg cattgcgctt ttccaggcag
     3721 ccatcgccga tcaggttggc ctcgttgtag gtcagttgca tgacccgcat gcccagcgca
     3781 tggaaaatgc cgatgcgctc gatcttgtcc tcgagcggac gggagttctg ccagcccatg
     3841 accaggccga ccttgccggc ggccttggcg cgctcgatat cctcggcctg ctcgatcagc
     3901 atccagcccg aggccgggtc ggcgcagcgc tcgcgccatg ccatcacgtc cttgagcgcc
     3961 tgctcgtagc cggaggccat gtcggtcacc gtcagattga gcgcgttgac gccgccggcc
     4021 ttcatgtcac gcgtgctgcc gtcgctgaaa aagaccagcc cgtcgacgat gctggccttg
     4081 cggtgcagtt cttctgccct gctcatcctc tcctcccatc ttctatttca atacttgtct
     4141 atcgactgcc gatagcttaa catcgttacg cgatatgatt agagcatcgc accgcgatgc
     4201 tgtcaaatgc ccatggagga agacgatgga agactcgccg cttttcatca gcgcactggc
     4261 caaggggctg tgcgttcttg aagcgttcaa cagcagcagc ccgacgatga acctgcccga
     4321 aatggcggcc gcctgcggca tcagcacgag cgccgcgcaa cgcattgcct tcacgctgca
     4381 gcagctcggc tacctgcgca aggacgagcg cagcaagcgt tacgggctga cggtgcgtgc
     4441 gatgtcgctc ggctacgcct acctgatgac cgagccgctg ctgcagcgcg cgcatccggt
     4501 gctcaacgag ctcgggcaag aatgcggcga gagcgtcaac ctgtccgtgc cggacggcaa
     4561 ggacatggtg ttcgtcacgc gcattccgtc gaagaagcac atacccgtct acatgccgat
     4621 cggcaagcgc attccggcca tctcgtccgc atcgggacgg gccgtgctgt cctgccgtgc
     4681 gagcgacgag gtcgagcgct tcatcgacag cagccccatc gagcgcttca cctcgcgcac
     4741 gaccgtggac cgggaacagt tgctggcgct cgtggccgaa gccgccgcga acggctatgc
     4801 ctacgccgcc gaggagtact acctggggga cttgaatgta gccgcggcag tcgtggatca
     4861 cgccggcatt ccgctcgccg cggtcaatgt ctcggtgccg acctcgcgct ggacgctcga
     4921 gcaggtgaag aaggatctgg gcccgctggt gatacgggcg gcgcgggcga tcggccagcg
     4981 cgcggactga ggcaccccgt ctgtcggagc ggggccgcaa gcagcaggat caggcgcaat
     5041 tcagggcgca attcagcccc gataccactc ttccagcctt ctttcctcga ccggccgcgg
     5101 agtgactggc cgctgcaccg gcggcgtcgg catggccgct ggcggcacca caatcggggc
     5161 cggcgccacc gacggtgcag gctgcgatgg ttgcgaaggc tgcgacggcc agcggaacag
     5221 gcgctcccag aaactgcgta tttcgtcgat gcccacggac cgtaccgaat tgccggccgc
     5281 ggcatactcc gcgtacatca ggtcgccgtc cacctgtgcc agtccgcccg gagcggcgcg
     5341 gtgcaccggt cccaccccgc gcaaggcgac acgcatgtaa tccgtccaga ttggcagcgc
     5401 cacggtgccg cc
//
LOCUS       DATLGF010000299         5527 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_181351, whole genome shotgun sequence.
ACCESSION   DATLGF010000299 DATLGF010000000
VERSION     DATLGF010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181351"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VM406_09130"
     CDS             complement(<1..444)
                     /locus_tag="VM406_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HVL76167.1"
                     /translation="MRYRLAVFDFDGTLADSFAFFLDTLDTLADEHGFRKLDRSQLDI
                     LRTLDARAVLKHVGLPLWKAPRVGAHYKRLMAAQAHRIPLFDGTDGMLRQLAAQGAAL
                     AILSSNSEENVRTVLGPELGGLFALAEQRAAAFVMREQAAKLRAED"
     gene            complement(444..1856)
                     /locus_tag="VM406_09135"
     CDS             complement(444..1856)
                     /locus_tag="VM406_09135"
                     /inference="COORDINATES: protein motif:HMM:NF013788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HVL76168.1"
                     /translation="MTNRSGRQAAVLRFLNWYLQGGRAALFLTPRWTHVHASPAAMAL
                     LAVGAMLVMATVDRLAIPGAAQFDWRAIGGGWFAAVIVFWACYLARPDARYDPNPRAA
                     PGPAWLICAALALQQIVLIATAALYATPLADAILAHAAFGAIGIWLLWLLPVIWATGA
                     LAVLLLRAGERQRRRRLLAMPVLAAAAVLAVLLPSDAYWREAASDSAGARPGLELTQE
                     LMEEQPRLLARRLAEMADQRPGHVDLYAITFAPYSDEDVFRNESAMVAEVIGQRFGAQ
                     ERTIQLVNHVETSADWPWATPLNLYRAIRHVAERMDRDEDILFLHLTSHGARNGQLAA
                     EFWPMTVHSLTPAMLKAWLDEVGIRHRILSISACYSGSWIDPLADENSLIMTAADAHH
                     TSYGCGRGSELTFFGRALFDEQLREHTLSFEDAHATAREVIRRREQEAGKNDGYSNPQ
                     IRIGPGIRQPLERLRNDVER"
     gene            2102..3631
                     /locus_tag="VM406_09140"
     CDS             2102..3631
                     /locus_tag="VM406_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL76169.1"
                     /translation="MQTRLSLRNITKAYPSVVANDGVDLDVKPGEIHSILGENGAGKS
                     TLMKIIYGSVKPDAGHVYWNGQEVHIANPAAARQLGIAMVFQHFSLFDTLTVAENIAL
                     GLPKDTNLDELSRRIETTARQYGLDLEAQRHVHTLSVGECQRVEIVRALLAKPQLLIL
                     DEPTSVLTPQAVEKLFETLRKLADEGCSILYISHKLDEIRALCHSCTVMRGGKVTGVC
                     DPTQETAASLSRMMIGAEPPEVKAVERVPGAKLLEVRGLSLERSHPFGTSLDNIAFDV
                     HAGEILGVAGVSGNGQQELLAALSGEDPRAAADAIVLDGRPIGRLNAARRRRLGLGFV
                     PEERLGRGAVPDLSLAHNILLSHQDSRTIRHGMVNFSAINRIAAGVIERFKVKAPGPH
                     ATAGSLSGGNLQKYIVGREVVREPKVLIVAQPTWGVDVGAAAQIRAELVALRDAGCAI
                     LVVSEELDELFEISDRMLVIAKGRVSPAIRRSDATVDMIGQWMSGLWDRAVRREEVAH
                     A"
     gene            3624..4691
                     /locus_tag="VM406_09145"
     CDS             3624..4691
                     /locus_tag="VM406_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL76170.1"
                     /translation="MLRLEKRPSPSALMSWTSPLLAVLLTALCGAILFAALGTDPLRG
                     LQVFFIEPVRDLRGWTEVGVKVAPLLLIAVGLAICFRANVFNIGAEGQLVVGAIVGGA
                     VALQLDPGDGEGGGALMIATILAAGALGGMVWAGIVAFLRDRFNANEILVSLMLVYVA
                     QLLLSYMVHGPLRDPDGFMFPQSRLLSDGFLLPELIEGYRLHFGIVLALLTAAAGWVF
                     LTRSYAGFRLQVGGMAPMAARYAGFSSRRALWTSLLACGALAGIAGTAEVIGPIGQLT
                     PSVSPGYGFAAIIVAYVGRLHPIGVVFSSFVMALFYIGGELSQSRLGMPNAITGVFQG
                     ILLFSLLACDVFIHYKLRWGK"
     gene            4697..>5527
                     /locus_tag="VM406_09150"
     CDS             4697..>5527
                     /locus_tag="VM406_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL76171.1"
                     /translation="MTTIAPLVAASINAGTPLMLAALGLLINEKSGVVNLGAEGMMLM
                     AAVVGFATAVNSGSIATGFIAGAAAGMLLAGLFALLAVWLGTNQYATGLALSLFGAGA
                     SAYIGISYSGHSLSAATTAHAVPFLSDIPVIGVALFRQHPMVYLSLLLCAAIIWFLYR
                     SRPGLVLRAVGESPTSAHSLGYNVRVIRTGALLFGGACCGLAGAYLSLVYTPLWVEGM
                     VAGRGWIALALTTFATWRPARVLIGAYLFGGVTILTFHAQGQGVAIPSQMLSMMPYLA
                     T"
BASE COUNT          860 a         1815 c         1889 g          963 t
ORIGIN      
        1 gtcctcggcc cggagcttgg cggcctgttc gcgcattacg aatgcggcag cacgctgttc
       61 ggcaagcgcg aacaggccgc caagctccgg gccgaggacg gtgcgcacgt tttcctcgga
      121 attggagctc aggatcgcca gggccgcgcc ttgcgcggca agttggcgca gcatgccgtc
      181 ggttccgtcg aaaaggggga tgcggtgcgc ctgcgccgcc atcagccgct tgtagtgcgc
      241 gccgacgcgc ggcgccttcc acagcggaag tccgacatgc ttcaacaccg cccgcgcgtc
      301 gagcgtgcgc aggatgtcga gctggctgcg gtcgagcttg cggaagccgt gttcgtcggc
      361 aagggtgtcg agcgtgtcga gaaaaaaggc aaacgaatcg gcgagcgtgc cgtcgaagtc
      421 gaatacggcc agccggtagc gcatcagcgc tccacgtcgt tgcgcaggcg ctcgagcggc
      481 tgccggatgc cggggccgat acggatctgc ggattcgagt aaccgtcgtt cttgcccgct
      541 tcctgctcgc ggcggcggat cacctcacgc gcggtggcgt gcgcgtcctc gaaggaaagc
      601 gtgtgttcgc gcaactgttc atcgaacagc gcacggccga agaacgtgag ctcggaaccg
      661 cgcccgcagc cgtaggaggt gtggtgggcg tcggctgccg tcatgatcag gctgttctca
      721 tcggcaagag gatcgatcca gctgccggaa tagcaggccg agatcgacag gatgcggtgg
      781 cggatgccta cctcgtccag ccatgccttg agcatggccg gggtgaggct gtgcaccgtc
      841 atcggccaga actcggcggc cagctggccg ttgcgggcgc catgcgaggt cagatgcagg
      901 aacaggatgt cttcgtcgcg atccatgcgc tcggcgacat gccggatcgc gcggtacagg
      961 ttgagcggcg tcgcccacgg ccagtcggcg ctggtctcta catgattgac gagttggatc
     1021 gtgcgctcct gcgcgccgaa ccgctgcccg atcacctcgg cgaccatcgc gctttcattg
     1081 cggaacacgt cttcgtcgga gtacggcgcg aaggtgattg catacaagtc cacgtgcccg
     1141 ggacgctggt ccgccatctc cgcaagtcgc cgcgccaaca ggcgcggctg ctcctccatc
     1201 agctcctggg taagttcgag acccggccgc gcgccggcgg aatcggaggc ggcctcgcgc
     1261 cagtacgcgt cggacggcag caggacggcc agcacggcgg ctgccgccag caccggcatg
     1321 gccagcaggc gccgccgccg ttgacgctcc ccggcgcgca gcagcagcac ggccagtgcg
     1381 cccgtcgccc agatcaccgg cagcagccag agcagccaga tgccgattgc gccgaacgca
     1441 gcgtgcgcga gaatggcgtc ggcaagcggc gtggcgtaca gcgcggctgt ggcgatcagc
     1501 acgatctgct gcagcgccag ggccgcgcag atcagccagg ccgggccagg ggcggcgcgc
     1561 ggattcgggt cgtagcgagc gtcggggcgc gcgagatagc aggcccagaa gacgatgacc
     1621 gcggcaaacc atccgccgcc gattgcgcgc cagtcgaatt gcgcagcgcc gggtatcgcg
     1681 agccgatcga cggtcgccat gaccagcatc gcgccgacgg caagcagcgc catcgcggcg
     1741 ggggaggcat gcacgtgggt ccagcgcggg gtgaggaaga gtgcggcgcg ccccccttgg
     1801 aggtaccagt tcaggaagcg gaggacggcc gcctgacggc cagagcggtt tgtcattatg
     1861 caagtccgtc ggagagatcg gcggcgatta tcctacaacg ccgcggcgtg catgcccgca
     1921 tggcattccg agcctcccgg ctgcaccgtc cgcgtgcgta tatccctcga tctatagaac
     1981 ccatacccgg gcccgtatat tgccaattgc tgaattcctg taatttgcgg caagtacggc
     2041 aacccccgat aacgaaaaaa cgctagcatc gcagctttgc ccgatgcgag gacgaggaga
     2101 catgcaaacc cgcctaagct tacgcaacat caccaaagcc tatccttccg tcgttgccaa
     2161 tgacggcgtc gacctcgacg tcaagccggg cgaaatccat tcgatcctcg gcgagaacgg
     2221 tgcgggcaag tccacgctga tgaaaatcat ctacggctcg gtcaagcccg atgccggtca
     2281 cgtttactgg aacgggcagg aggtgcatat cgccaacccc gccgccgcgc gccagctcgg
     2341 cattgcgatg gtgttccagc atttttcgct gttcgacacg ctgactgtcg cggagaacat
     2401 cgcgctcggc ctgccgaagg acacgaacct ggatgaactg agccgccgca tcgaaaccac
     2461 cgcccgccag tacgggcttg atctcgaggc gcagcggcat gtgcatacgc tttccgtcgg
     2521 cgaatgccag cgggtcgaga tcgtgcgcgc actgctggcg aagccgcaat tgctgattct
     2581 cgacgagccg acctcggtgc tcaccccgca ggctgtcgaa aagctgttcg agacgctgcg
     2641 caagctcgcg gacgaaggct gcagcattct ctacatcagt cacaagctcg acgagatccg
     2701 tgcgctgtgc cattcgtgca cggtgatgcg cggcgggaag gtcaccggcg tttgcgatcc
     2761 gacccaggag acggccgcca gcctgtcgcg catgatgatc ggtgccgagc cccccgaggt
     2821 gaaggcggtc gagcgcgtgc ccggcgcgaa gctgctcgag gtgcgcggac tctcactcga
     2881 gcggagccat cctttcggca ccagtctcga caacatcgca ttcgatgtcc acgccgggga
     2941 gatcctcggt gtggccggcg tctccggaaa cggccagcag gaactcctgg ccgcgctgtc
     3001 gggcgaagac ccgcgcgccg cggccgacgc gatcgttctc gacggcaggc cgatcggcag
     3061 gttgaatgcg gcgcggcggc gccgtctcgg cctgggcttc gttcccgagg aaaggctggg
     3121 acgcggcgcg gtcccggatc tctcgctcgc ccacaatatc ctgctgtccc accaggacag
     3181 cagaacgatc cgccatggca tggtcaattt cagcgcgatc aatcgcatcg cagccggagt
     3241 catcgagcgc ttcaaggtca aggcgccggg gccgcacgcg acggccggca gcctctccgg
     3301 cggcaacctg cagaaataca tcgtcggacg cgaggttgtg cgcgaaccca aggtgctgat
     3361 cgtcgcccag ccgacctggg gcgtcgacgt cggcgccgcg gcgcagatcc gcgccgagct
     3421 ggttgcgctg cgcgacgccg gctgcgcgat cctcgtcgta tcggaagagc tcgacgagct
     3481 gtttgagatc tcggaccgca tgctggtcat cgcaaagggg cgcgtgtcgc cggcgatccg
     3541 gcgttcggat gcgacggtcg acatgatcgg ccaatggatg agcgggctgt gggaccgggc
     3601 cgtgcgccgc gaggaggtgg cccatgctta ggctggagaa gcggccgagt ccgtcggccc
     3661 tgatgtcgtg gacgtcccct ctgctcgcgg tactgctgac ggcgctgtgc ggagcgatcc
     3721 tgttcgccgc gctcggcacc gacccattgc gcggcctgca ggttttcttc atcgagccgg
     3781 tacgcgatct gcgcggctgg accgaagtcg gcgtgaaggt ggcgccgctg ctgttgatcg
     3841 cggtcggcct ggccatctgc tttcgtgcga acgtgttcaa catcggcgcc gagggacagc
     3901 tggtggtggg cgcgatcgtc ggcggcgcgg tggcgctgca gctcgaccct ggtgacggcg
     3961 agggcggcgg cgcgctgatg atcgcaacga tcctggccgc cggcgcgctc ggtggcatgg
     4021 tctgggcggg catcgtggcc ttcctgcgcg accgtttcaa tgccaacgag atcctggtct
     4081 cgctgatgct ggtgtacgtc gcgcagctgc tgctgagcta catggtgcat ggaccgctgc
     4141 gcgatccgga cggcttcatg ttcccgcaat cgcgcctgct gtccgacggc ttcctgctgc
     4201 ccgagctgat cgaaggctac cgcctgcact tcggcatcgt gctggcgctg ttgaccgcgg
     4261 ccgccggctg ggtgttcctc acgcgcagct atgccggctt ccgcctgcag gtcggcggca
     4321 tggcgccgat ggcggcgcgc tatgcgggct tctcgtcgcg ccgcgcgctg tggacgtcgt
     4381 tgctggcctg cggcgcgctg gccggcatcg ccggcaccgc agaagtgatc gggcccatcg
     4441 gccagctgac gccgtcggtc tcgcccggct acggctttgc cgcgatcatc gttgcctacg
     4501 tcggccggtt gcatccgatc ggcgtggtgt tctccagctt cgtgatggcc ttgttctata
     4561 tcggcggcga actgtcgcag tcgcgcctgg gcatgccgaa cgcgatcacc ggcgtgttcc
     4621 agggcatcct gctgttctcg ttgctggcct gcgacgtgtt catccactac aaactgcgct
     4681 gggggaaatg atggacctga ctaccattgc gcctttggtc gcagcgtcca tcaatgcggg
     4741 tacgccgctg atgctcgccg cgctgggtct cttgatcaac gaaaagtccg gcgtcgtcaa
     4801 tctcggcgcg gaaggcatga tgctgatggc ggcggtggtc ggcttcgcca cggctgtcaa
     4861 cagcggcagc atcgcgaccg gcttcatcgc cggtgcggcg gccggcatgc tgctggccgg
     4921 cttgttcgcg ctgctcgcag tctggctcgg caccaatcag tacgcgaccg gactcgcgct
     4981 gtcgttgttc ggcgccggcg cctccgccta catcggcatc agctactccg gccacagcct
     5041 gtcggcggcc accaccgccc atgcggttcc gttcctgtcg gatatcccgg tgatcggcgt
     5101 ggcgctgttc cgccagcatc cgatggtcta cctgtcgctg ctgctgtgcg cggccatcat
     5161 ctggttcctg taccgcagcc ggccgggcct ggtgctgcgc gcggtcggcg agtcgcccac
     5221 ttccgcccat tcgctcggct acaacgtgcg cgtcatccgg accggcgcat tgctgttcgg
     5281 cggcgcctgc tgcggcctgg ccggcgccta cctgtcgctg gtgtacacgc cgctgtgggt
     5341 cgaaggcatg gtggccgggc gcggctggat cgcgctggcg ctgaccacct tcgcgacctg
     5401 gcgtccggcg cgcgtgctga tcggcgccta cctgttcggc ggggtgacga tcctcacctt
     5461 ccacgcgcag gggcagggcg tggcgattcc gtcgcagatg ctgtcgatga tgccctacct
     5521 cgcaacc
//
LOCUS       DATLGF010000300        14768 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_228416, whole genome shotgun sequence.
ACCESSION   DATLGF010000300 DATLGF010000000
VERSION     DATLGF010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 14768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14768
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228416"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="VM406_09155"
     CDS             complement(<1..1295)
                     /locus_tag="VM406_09155"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HVL76172.1"
                     /translation="MLEPYRPRPAALFLCACLLACGSGRAAATRLVDLSLEQLSEIVV
                     TSVSRRDERLSDAAASIYVIRGEDIRRSGAGSLPEALRLAPNLQVARVNASDYAISAR
                     GGNSTTANKLLVLIDGRTVYTPLFSGVFWDAQAVMLADIERIEVISGPGTTMWGANAV
                     NGVINIITRNAADTVGTLAETRVGQRERGVAVRHGTALGEQAHLRIYGMSSQSDATRR
                     ADGSLGGDRASHAQAGWRADWQRSGDHLTLQGDVYRARTGRTPGGPELAGANLLGRLS
                     RRLDGDQDLVLQAYLEHSEREVPRSFHQRLDAVDVEFQHALTPAPAHRLMWGAGLRHA
                     RDRVDSGPVIAFLPPSRTLNHAHVFVHDQWTLSDRVGLGAGLKLEKNPDTGIEVMPDL
                     RLTWRIGEAGLAWGTLARAVRAPARLDRDLYVPAVGGART"
     gene            complement(1436..2467)
                     /locus_tag="VM406_09160"
     CDS             complement(1436..2467)
                     /locus_tag="VM406_09160"
                     /EC_number="7.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006119 - oxidative phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HVL76173.1"
                     /translation="MTMIPELNQPEVWLPLAFYALLGVAMLAYVVLDGFDLGVGILLR
                     RADDAQKDRMIASIGPFWDANETWLVLGVGILLVAFPMAHGVILGALYLPVALMLAGL
                     ILRGVAFDFRVKARDDHKAAWNLAFYGGSVLATFSQGLMLGLYITGFVYEGAQLLFAI
                     FTGLALVAGYALLGATWLIMKAEGELQRRAIAWARGSLWLCALGVAAVSLATPLVSQQ
                     VFDKWFAFPNLLLLAPIPVVTAALFFITDYTLRKLPDLLAADSHAFCWLPFAATVGVF
                     VLAFFGLAYSLFPYLVVDRITVWDAASAPASLKIILAGVLIVLPTIVAYTAFSYRVFW
                     GKARELSYQ"
     gene            complement(2464..3846)
                     /locus_tag="VM406_09165"
     CDS             complement(2464..3846)
                     /locus_tag="VM406_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HVL76174.1"
                     /translation="MTDALLLARIQFASNITFHILFPTITIALGWVLLYFKLRFNRSG
                     DVRWSQAYQFWVKIFALTFALGVVSGITMSFQFGTNWPGFMETVGNIAGPLLAYEVLT
                     AFFLEATFLGIMLFGAQRVSNRVHTLATFLVAAGTTMSAFWIIVLNSWMHTPAGFEMI
                     DGQAHATDWLAIIFNPSMPYRLTHMLIASGLTVAFLIAGLSAYRWLRGDRAPAVMAAL
                     KTGVALGAFLIPLQIFVGDMHGLNTLKYQPAKIAAIEGIWHTERGAPLLLFALPDTAE
                     RANRYEIGIPKLASLILTHELDGELQGLEAFEGRHPPVAPVFWGFRVMVGIGMLMLAL
                     SWLAAWQLKRRGEPAPWTARALVAMSFSGWVATVAGWYVTEIGRQPYLVQGVLTTAEA
                     TSTVPAPMIASTLAMYLSLYLALTVAYVTVLFHLARKAGKGDAEAYPAPRPDAPALAP
                     APVATMTMKG"
     gene            complement(3860..4444)
                     /locus_tag="VM406_09170"
     CDS             complement(3860..4444)
                     /locus_tag="VM406_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HVL76175.1"
                     /translation="MNLPPLVQTFVLHFGEMGSRWGINRTVGQIYALLYLSDKPLNAE
                     EIAEALGFSRSNVSMGLKELSAWNLVRLQHLPNDRREYFSTPEDVWEIFRTLAEERRK
                     REIDPTLSMLRNALIQPPSSDEERHAQERMKDMYELVELVTQWFGDIQRLDAATLVQL
                     MKLGSKAQKLLEMKNKLTIVGQRKSARAADKDAN"
     gene            4590..6308
                     /locus_tag="VM406_09175"
     CDS             4590..6308
                     /locus_tag="VM406_09175"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVL76176.1"
                     /translation="MKRSTLQRVLVIPFVILVLSLGGVIYLATKRAGDNASHEFSRTV
                     LLGLSERIGQATSHHLLGAHIAIKAVVPDPVYSPVENRTTALPFPDDLQGLEERLWIA
                     TGFFPFVNNYVYFGTPDGRFVGINRRPDRTELRLQAAGGAPRTVYSVAAPGERLGVVR
                     TDNYDARQRPWYVSAVKKGHATWSAVYTDFSTLEPTLTLARPVYRADRSLIGVVATDM
                     ALTDLTDFMRGLSVSRTGVAYIVERDGAIIATSSGEMPFRIEADTLRRVMANESDSPL
                     LRESHAAVIGWLQDGWDPATARSREITTSSGSVQIAATQLRDDAGLDWLTVVAVPRAD
                     FLSNLSGVLYQSLAIGLAAVFLVLVLGFLLLQWVLRDIRKLTLAAKSIGRGEPLEPLD
                     IRRRDEIGELAVSFQEMERNLRTDHLTGVLNRETLLNHIAVRVHGDSQGAWHPFALLF
                     IDLDGFKAINDTHGHDAGDKILVDVARRLKFALRSTDRVARFGGDEFVVYLGGVSSEH
                     EAQAVAEKIVLAVEEPTMLDDGASVQVGASIGMAFYPADGRDLEAIIKAADTRMLDLK
                     RARKAA"
     gene            complement(6383..8290)
                     /locus_tag="VM406_09180"
     CDS             complement(6383..8290)
                     /locus_tag="VM406_09180"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVL76177.1"
                     /translation="MNTVVRRLASKASGRFWRLPLAAQLALCLLAVLPAVWIAVMLQL
                     YQLERLSESESRTELRNLAHAFADSVDAAIDTIDLSLGALRGHWGRDPDDISAAVGHL
                     QARMHVSVGFDIGFVDTDGVLQFSTAGRAAQALDLSDRDYVQIPLRSRIDQLFIGTPV
                     IDRLSDRWIIPFSRPVYAENGDLNGVIVLSAVPGYFSSFYSRIDLGERAAVTLVRSDG
                     RILARSPDDEAALGRHLRGAALAAGGREGAGFYQAVSEVDGIERFHAWRALPESDLIV
                     VVGKSMSDMHSRYAEQRAANITGGIVISLLLTWVAYILLAGGRNRARTNARLAESEAR
                     WAFALEGSEQGVWDWDIVRRRVQLSARSRELLGIDDDSVPGTPEALLRYVYPDDAETV
                     QSGLEGQIKGESATFVAEHRVRRGDGVVRWILSRGMVAERDEDGRAVRMVGTFSDITD
                     RKSKEAAVLHRAQHDSLTGLPNRATFLDRLEQALARARREGSELAVMFFDLDRFKPVN
                     DTYGHHVGDLLLREVAERAASCLRRSDTVARLGGDEFTVLLPRIASEADALTVAEHIR
                     AALAQPFRIGELELHVAASIGVASFPSDGDSVGSLTKAADEAMYVAKAAGRNTVRTVR
                     PAPVSAAARAG"
     gene            8538..9470
                     /gene="cysK"
                     /locus_tag="VM406_09185"
     CDS             8538..9470
                     /gene="cysK"
                     /locus_tag="VM406_09185"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HVL76178.1"
                     /translation="MKIANDVTELIGNTPLVRLNRMTEGCAAEVLAKLEFFNPAHSVK
                     DRIGASMIDAAEQAGLIGPDTIIVEPTSGNTGIALAMVCAARGYRCVLTMPETMSRER
                     RMILRAYGAELILTPGPDGMGGAIRKAEELVASDKRYFMPQQFKNPANPEVHRRTTAE
                     EIWRDTDGRADILISGIGTGGTITGVGEVIKSRKPEFRCVAVEPDASPVLSGGSKGPH
                     PIQGIGAGFVPDILNTEVYDEIIRVKNEDAFAFARRAAREEGLLVGISSGAALWAALE
                     VARRSENAGKLIVVIIPSFGERYLSTPLFADLAD"
     gene            9718..11637
                     /locus_tag="VM406_09190"
     CDS             9718..11637
                     /locus_tag="VM406_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVL76179.1"
                     /translation="MPTFSKVFESTKNIYVRIGLVAVVALIALVIAFRPMSTAEPTTV
                     VQSQDITEITRLAAEKERLEYLLLANPLSNFPRYVFKYKDAPQIHVVKVPSTSHQNLE
                     REVLLKNGIPYAIASEQFLETHKEALKDEATRRETMSEVGMFIARNAVGLLLIGFLIY
                     FFRKGVPGMSASASLIKPESLKGSIDDLVGMEDIKREVAHLEEMIRDRDSYRSHNIDK
                     PFNVMLTGPAGTGKTKLAGYLAKKLNIPLIQASGSGLESGFVGGGAKALNALYKKACA
                     QERCIIFLDEAQTLFMPRGRGDKKWDDDTANTLLGLLDGIKSNEGSGVIWIVASNFDD
                     ASMQMDEAMLRRFSVKINFRLPNRSERRELVRVFLSRKDDRCVDWNDLNLDYVAEITA
                     NLSPALIENVVDRASMIAIQEKTVINTDVLFRAFERAAIGLTDRATTADKTKERERIA
                     LHELGHFFMQIDPYLKKGMSWAEVKEKSHLLKISTEAVSKIGALGYVLSSQDDVALRT
                     LEELEQEVIHLYGGVAAEELFYGARGISTGSQNDIQKVTALLDNMVNRLSMYKHSKLD
                     YRQLKGDAGQEDAIRQVEAKADELYVRTLEATKEYRDLMWALKDTLLDRYVLSKDEVF
                     ALLEEMTMEREEQAA"
     gene            11940..13403
                     /locus_tag="VM406_09195"
     CDS             11940..13403
                     /locus_tag="VM406_09195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HVL76180.1"
                     /translation="MRRRRTPARIARLTLPGLAAMLLACTTPPLSTPEIAASLPDAYR
                     SGSAAGPILDAWWTRFNDARLNALVLHAAQDSPSVAQAAARLRIAQAQARAARADFSP
                     QVSAGFNAARQRQNLAGLGPIGELPLAPGDAAGESASFTTSNFGLSLDISWELDLWGR
                     IAAQSAAARADFLASRENLRAVRQSVAAQTARLYFTVTEARAQVDLAERTAATIGEIA
                     RQINNRADAGIAAPSDRLLANANLESARSGLQQRREALERATRQLEVLLRDYPAGGIA
                     TASVLPEVPPPAPAGLPADLLKRRPDILASELALHAAGYRLTAAERTLLPNIALTGSA
                     GTASAELGNLLSGGSVVWTIAGRVLQPIFQAGRIRAQVDIVEGQRAEAIEAYADTVLR
                     ALAEVETALAVDTLLAQRESALDAAASAAEGAVAISFNRYREGIDPFLAVLESQQRAL
                     DSRSAYLAARRARLENRIDLHLALGGGFEPAPDAKQE"
     gene            13404..>14768
                     /locus_tag="VM406_09200"
     CDS             13404..>14768
                     /locus_tag="VM406_09200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVL76181.1"
                     /translation="MMLIDLLRSLFPSSPCLRRATQWSSDAKRRHPALQRAARAAMVV
                     TIACWSTACGRADAQQPAPAQEAGAVSVRVVPAMPAAVQAWLYGQGTARAHRREFLSF
                     ANQGRVTYVDPKLRVGSRVKKGQLIAYQEPDRVRADLASARAGLAGAQAELAAARAAL
                     SEAEANLELARVTHERYLVLIAQNSASRQELDQARAQLEQARAAYRRAEVQIKSSEAQ
                     VNASRSQVAQAQVIVSESRLVSPIDGVLARLNIEQGRYFTPQVVQTGTEQGALRTVPA
                     VVIDPSAFEITVDLPAYAFRQIEEGAAVVIGHARAQAPPTIGGSAGGPQAAQAQQAAP
                     APEGELQGRVHAVSPSLDPETRTFQVIIRTTTETPRLQDGEFVNVWIAGQARDQVLAV
                     PLEALRYQAGQAFLFVVDPQSGIARERRVALGQIEGNRQAVLSGLSEGELVVTEGRAR
                     LNDGQ"
BASE COUNT         2624 a         4995 c         4874 g         2275 t
ORIGIN      
        1 gtgcgggccc cgccgacagc cggaacgtac aggtcccggt ccaggcgcgc cggggcccgc
       61 acggcccgcg cgagcgttcc ccaggccaac cccgcttccc cgatgcgcca ggtcaggcgc
      121 agatccggca tcacctcgat accggtgtcg ggattcttct cgagcttcag accggcgccg
      181 agtccgaccc ggtccgagag cgtccactga tcgtgaacga acacatgggc atggttgagc
      241 gtgcgcgagg ggggcaggaa cgcgatcacg gggccgctgt cgacgcggtc gcgggcgtgg
      301 cgcagtccgg cgccccacat cagccggtgt gccggggccg gggtcagcgc gtgctggaat
      361 tccacgtcga cggcatcgag gcgctggtgg aagctgcgcg gcacctcgcg ctcgctgtgc
      421 tccaggtagg cttgcagcac gaggtcctgg tcgccgtcga gacggcgaga aagccgccct
      481 agcaggttgg cgcccgccag ttcggggccg cccggcgtgc ggccggttct ggcccggtag
      541 acatcaccct gcagcgtcag gtggtcgccg ctgcgctgcc agtccgcgcg ccagccggcc
      601 tgcgcatggc tggcccggtc gccaccgagg gagccgtcgg cgcgccgggt cgcatcgctt
      661 tgcgaactca ttccgtaaat gcgcagatgc gcctgctcgc ccagcgccgt gccgtgccga
      721 accgccacgc cgcgttcccg ctgtccgacg cgggtttccg ccagcgtccc gacggtgtcg
      781 gcggcgttgc gggtgatgat attgattacc ccgttgaccg cgttggcgcc ccacatcgtg
      841 gtgcccggcc cgctgatgac ctcgatgcgc tcgatgtcgg cgagcatgac cgcctgggca
      901 tcccagaaca cgccggagaa caatggggtg tagacggtac gcccgtcgat cagcaccagc
      961 agcttgttgg ccgtcgtgct gttgcccccg cgtgcgctga tcgcatagtc ggaagcgttg
     1021 acccgcgcaa cctgcaggtt cggcgccagc cgcagcgcct ccgggaggct gccggcgccg
     1081 ctgcggcgga tgtcctcgcc acggatcaca tagatcgagg cggcggcgtc ggacaggcgc
     1141 tcatcgcggc gcgacaccga ggtcacgacg atctcgctca gctgttcgag ggacaggtcg
     1201 accagacggg tggcggcggc ccggccgctg ccgcaggcca acaggcaggc gcagaggaac
     1261 agggccgccg gccggggacg ataaggttcg agcatgaaag agacgcaggc attcgcggtc
     1321 ccttccttgg gcaagcttgc cacgacggta accgcatagt ttccgtgagg ctacataatt
     1381 ccttgcgcac cggattgaga atcagcaaat ccggtgcgtc gctgtcgcgc gcctgctact
     1441 ggtagctcag ttcccttgcc ttgccccaga acacgcggta ggaaaatgcc gtgtacgcca
     1501 cgatggtcgg cagaacgatc aggacgcccg ccagaatgat cttcagcgat gccggcgcgc
     1561 tggccgcgtc ccagaccgtg atgcggtcga ccaccaggta cgggaacagg ctgtaggcca
     1621 gcccgaagaa ggccagcacg aacacgccga cggtggccgc aaacggcagc cagcagaacg
     1681 catgactgtc ggcggcgagc aggtcgggca gcttgcgcag cgtgtagtcg gtgatgaaga
     1741 aaagcgccgc ggtgacgacc ggtatcggcg ccagcagcaa caggttcgga aacgcgaacc
     1801 acttgtcgaa cacctgctgg ctcaccaggg gcgtcgcgag cgaaaccgcc gccactccga
     1861 gcgcgcacag ccacaggctg ccgcgcgccc atgcgatcgc acgacgctga agttcgccct
     1921 cggccttcat gatcagccag gtcgccccga gcagcgcata gccggccacc agcgccagcc
     1981 cggtgaagat cgcgaacagc agttgcgcgc cttcgtagac gaaaccggtg atgtacaggc
     2041 cgagcatcag cccctgtgag aaggtggcca gaaccgatcc gccgtagaag gccaggttcc
     2101 acgcggcctt gtggtcgtcg cgcgccttca cacggaagtc gaaggccacg ccgcgcagga
     2161 tcaggccggc cagcatcagc gccaccggca ggtagagcgc gccgaggatc acgccgtggg
     2221 ccatcgggaa cgcgaccagc aggatgccca cgcccagcac cagccaggtt tcgttggcat
     2281 cccagaacgg accgatggac gcgatcatcc ggtctttctg cgcgtcgtcg gcgcgccgca
     2341 gcaggatgcc gacgccgagg tcgaagccgt ccagcaccac ataggcgagc atggccacgc
     2401 cgagcagcgc gtagaacgcc agcggcagcc agacttcggg ttggttcagt tcgggaatca
     2461 tggtcagccc ttcatcgtca tggtggcaac aggggcaggg gccagcgccg gcgcatccgg
     2521 gcgcggcgcg ggataggctt cggcatcgcc cttgccggcc ttgcgcgcga ggtggaacag
     2581 cacggtcaca taagccacgg tcagcgccag gtacagcgac aggtacatgg ccagggtgga
     2641 ggcgatcatc ggcgcaggca ccgtggaagt ggcctcggcg gtggtgagca cgccctgcac
     2701 cagatagggc tggcgaccga tctcggtcac gtaccagccg gccaccgtgg cgacccaacc
     2761 ggaaaagctc atcgccacca gcgcgcgggc ggtccacggc gccggttcgc cgcgccgctt
     2821 cagctgccag gcggccagcc aggagagggc cagcatcagc atgccgatgc cgaccatcac
     2881 gcggaaaccc cagaacaccg gcgcgaccgg cggatgcctg ccctcgaacg cttccagccc
     2941 ctgcagctcg ccgtcgagtt catgggtgag gatcaggctg gcgagtttgg ggatgccgat
     3001 ttcgtagcgg ttggcccgct cggccgtatc cggcagcgcg aacagcaaga gcggcgcgcc
     3061 gcgctcggta tgccagatgc cctcgatggc cgcgatcttg gccggctggt acttgagcgt
     3121 attcaggccg tgcatgtcgc cgacgaagat ctgcagcggg atcaggaagg cgccgagcgc
     3181 cacgccggtt ttcagtgcgg ccatcacggc cggcgcgcga tctccgcgca gccagcggta
     3241 ggcggacagg ccggcgatca ggaaggccac cgtcaggcct gaggcgatca gcatgtgcgt
     3301 gagccggtac ggcatcgacg gattgaagat gatcgccagc cagtcggtcg cgtgcgcctg
     3361 gccgtctatc atctcgaagc cggccggcgt gtgcatccac gagttgagca cgatgatcca
     3421 gaacgccgac atcgtcgtgc cggcggcgac caggaaggtc gccagcgtgt gcacgcggtt
     3481 ggagacgcgc tgcgcgccga acagcatgat gccgaggaag gtcgcctcca ggaagaatgc
     3541 cgtcagcacc tcataggcca agagcgggcc ggcgatgttc ccgaccgttt ccatgaagcc
     3601 cggccagttg gtgccgaact ggaaactcat cgtgataccc gagaccacgc ccagcgcgaa
     3661 cgtcagcgcg aagatcttga cccagaactg gtaggcctgc gaccagcgca cgtcgccgct
     3721 gcggttgaag cgcagcttga agtagagcag cacccagcca agcgcgatcg tgattgtcgg
     3781 gaacaggatg tggaaggtga tgttgctggc gaactggatg cgcgccagca gcagtgcgtc
     3841 ggtcatggca gctccttggt caattggcgt ccttgtcggc ggcgcgcgcg ctcttgcgct
     3901 ggccgacgat cgtcaacttg ttcttcatct ccagaagctt ctgggccttg gagccgagct
     3961 tcatcagctg caccagggtc gcggcgtcca gccgctggat gtcgccgaac cactgggtga
     4021 ccagctcgac cagctcgtac atgtccttca tgcgctcctg cgcatggcgc tcctcgtcgc
     4081 tggacggcgg ctggatcagc gcgttgcgca gcatcgacag cgtcgggtcg atctcgcgct
     4141 tgcggcgctc ctcggccagc gtgcggaaaa tttcccacac gtcttcgggt gtggagaagt
     4201 actcgcgccg gtcgttcggc aggtgctgca gccgcaccag gttccaggcc gacagctcct
     4261 tcaaacccat gctgacgttg gaacgggaaa agccgagggc ctcggcgatc tcctcggcgt
     4321 tcagcggctt gtcggacaga taaagcagcg catagatctg cccgacggtg cggttgatac
     4381 cccagcggct gcccatttca ccgaaatgga ggacgaaggt ctggacgagt ggcggcaggt
     4441 tcattgatct ggattatgga atttcaggaa tttctgaaat tttagacgat attggacccc
     4501 tgtggtggtc ggcggcttcc cgcgccaact tattgttgta cggcaattca aaccggagta
     4561 cgctgtccgg gctcactccc gccctgtcaa tgaaacgatc caccctccaa cgggtactgg
     4621 tcatcccgtt cgtgatcctt gtgctctcgc ttggaggggt gatctatctc gccaccaagc
     4681 gcgccggcga caacgccagc cacgagttct cccgcaccgt cctgctcggc ctgtccgaac
     4741 gcatcggcca ggccaccagc caccatctgc tgggagccca catcgcgatc aaggcggtgg
     4801 tgccggaccc cgtctattcg ccggtcgaga accgtacgac cgccctgccc tttcccgacg
     4861 atctgcaagg gctggaggaa cggctctgga tcgcgaccgg gttcttcccg ttcgtgaaca
     4921 actatgttta cttcggcacg ccggacggcc gcttcgtcgg tatcaaccgg cgtccggacc
     4981 gtaccgaact gcgtctgcag gcagccgggg gcgcgccgcg caccgtgtac agcgtggccg
     5041 ctcccggcga gcgtctgggc gtggtccgca cggacaacta cgatgcgcgc cagcgcccct
     5101 ggtacgtcag cgcggtcaag aaggggcacg cgacctggtc cgccgtctat accgatttct
     5161 ccaccctcga accgacgctc acgctcgcca ggccggtata tcgcgccgac cgcagcctga
     5221 tcggcgtggt tgccaccgac atggcgctga cggacctgac cgacttcatg cgcggtctct
     5281 cggtcagccg taccggggtg gcctacatcg tcgagcgcga cggggcaatc atcgccacct
     5341 cctccggcga gatgccgttc cggatcgaag ccgacacact gcgccgggta atggccaacg
     5401 aaagcgattc gccgctgctg cgcgagtcgc acgcggccgt catcggctgg ctgcaggatg
     5461 gatgggaccc ggccacggcg cgctcgcgcg aaatcacaac ctcgtccgga tccgtgcaga
     5521 tcgccgcgac ccagctgcgc gacgacgccg gcctggactg gctgacggtg gtggcggtgc
     5581 cgcgcgccga tttcctcagt aatctgtccg gcgtgctgta ccaaagcctg gcgatcggcc
     5641 tggcagcggt cttcctcgtg ctggttctgg gtttccttct gctgcagtgg gtactgcgcg
     5701 atatccgcaa gctcaccctc gccgcgaaga gcatcgggcg gggtgagccg ctcgaaccgc
     5761 tcgatatccg ccggcgcgac gagatcggcg aactggcggt gagcttccag gaaatggaac
     5821 gcaacctgcg caccgaccac ctcaccggcg tgctcaaccg cgagaccttg ctgaaccata
     5881 tcgcggtgcg ggttcacgga gacagccagg gcgcctggca tccgttcgcg ctgctgttca
     5941 tcgacctgga cggcttcaag gcgatcaacg acacccacgg acacgatgcc ggcgacaaga
     6001 tccttgtgga cgtcgcgcgc cggctgaaat tcgcgctgcg ctcgaccgac cgtgtcgccc
     6061 gcttcggcgg cgacgaattc gtcgtctacc tgggcggcgt gtccagcgag cacgaagcac
     6121 aagcggtggc ggagaagatc gtcctggcgg tcgaagaacc gacgatgctc gacgacggcg
     6181 ccagcgtgca ggtcggcgcc tcgatcggca tggcgttcta tccggccgac ggccgcgacc
     6241 tcgaggcgat catcaaggcc gcagacacgc gcatgctcga tctgaagcgc gcacgcaagg
     6301 ccgcctgatc cgggcggccg gcgaaccgat ccgcccccgc cccattgcgg ggaggcgctt
     6361 gcgcgttact gagaagtcgc cctcaaccgg cccgtgcggc cgcgctgacc ggtgcggggc
     6421 ggacggtgcg caccgtattg cgtcccgccg ccttggccac gtacatcgcc tcgtccgcag
     6481 ccttggtcag cgatccgacg ctgtcgccgt cgctcgggaa gcttgccacg ccgatcgagg
     6541 ccgccacatg cagttccagc tcgccgatgc gaaacggctg ggcgagcgcg gcgcggatat
     6601 gttcggccac cgtgagcgca tcggcttcgc tggcgatgcg cggcagcagc acggtgaatt
     6661 cgtcaccgcc caggcgcgcc accgtatcgg agcggcgcag gcaggacgcc gcccgctcgg
     6721 cgacctcgcg cagcagcagg tcgccgacat gatggccgta ggtatcgttg accggcttga
     6781 atcggtccag gtcgaagaac atcacggcca gttcgctgcc ttcgcggcgg gcccgggcca
     6841 gtgcctgttc gagacggtcg aggaaggtgg cgcggttcgg caggccggtg aggctgtcat
     6901 gctgcgcccg gtgcaggacc gcggcctcct tcgatttgcg atcggtgatg tcgctgaacg
     6961 tgcccaccat gcgcaccgcc ctgccgtcct cgtcgcgttc ggcgaccatg ccgcgcgaca
     7021 ggatccagcg caccacgcca tcccccctgc ggaccctgtg ctcggcgacg aaggtggcgg
     7081 actccccctt gatctgcccc tcgagcccgg actgtaccgt ctcggcatcg tccgggtaca
     7141 cgtagcgcag caatgcctcg ggcgtgccgg gcacactgtc atcgtcaatc ccgagcaact
     7201 ccctgctgcg cgccgacagc tgcacgcgcc gccggacgat gtcccagtcc cacacgcctt
     7261 gctccgagcc ttccagtgcg aatgcccagc gcgcctcgct ctcggccagc cgcgcgttcg
     7321 tgcgtgcacg attgcgccca ccggccagca ggatgtacgc gacccaggtg agcagcagcg
     7381 aaatcacgat gccgccggtg atgttggcgg cgcgctgctc ggcgtaacgg gagtgcatgt
     7441 ccgacatcga tttgcccacc acaacgatca ggtcggattc cggcagcgcg cgccatgcat
     7501 ggaagcgctc gatcccgtcc acctcggaga ctgcctggta gaagccggct ccctcgcgcc
     7561 cgcctgctgc cagcgcggcc ccgcgcagat gccgcccgag ggcggcttcg tcgtcgggag
     7621 agcgcgccag gatgcgcccg tccgaccgaa ccagcgtgac cgcggcacgc tcgcccagat
     7681 cgatgcggct gtagaaactc gaaaaatagc ccggaaccgc ggacagcacg atcacgccgt
     7741 tgaggtcgcc attctccgca tacaccggcc gggagaaagg gatgatccag cgatccgaca
     7801 gccggtcgat caccggcgtg ccgatgaaga gctggtcgat ccggctgcgc agcgggatct
     7861 gcacgtagtc gcgatcgctc agatccagcg cctgcgcagc gcggccggcg gtggaaaact
     7921 gcaagacgcc gtcggtatcc acgaagccga tgtcgaaacc gacgctcacg tgcatccgcg
     7981 cctgcaggtg tccgactgcc gcgctgatgt catcgggatc gcgcccccaa tggccgcgca
     8041 acgcccccaa cgacaggtcg atggtgtcga tcgcggcatc gacgctgtcg gcgaacgcgt
     8101 gcgccagatt gcgcagctcg gtgcggcttt ccgactcgga cagtcgttcc agctggtaaa
     8161 gctgcagcat caccgcaatc cagactgcgg gcaggacggc aagcagacaa agcgcgagct
     8221 gggccgcgag cggcagccgc cagaatcggc cgctggcctt gctggccagg cggcgaacaa
     8281 ccgtattcat gtggtaagtc ctgccacctt gatcgtcgcg gtgttcctgt gcggagcggg
     8341 tggcggatgc aaagcaagat gtgaagggga acgccccatc atactttccc tgcggcaaaa
     8401 atcaagatcg gagcatcgcc gatacgtgcc acctcacagc ttgaggtgga tcaagcagat
     8461 tcaaggtggc cgcgatcgcg ggcgaacgtc cggatgccca ggcgataaca tccatggttc
     8521 ccgtaccgga gacccaaatg aagattgcaa acgacgtcac tgaactcatc ggcaacacac
     8581 cgctggtgcg cctgaaccgc atgacggaag gctgcgccgc cgaagtcctt gccaagctgg
     8641 aattcttcaa tcccgcgcac agcgtgaagg accgcatcgg ggcctcgatg atcgacgccg
     8701 ccgagcaagc gggtttgatc ggcccggaca cgatcatcgt cgaacccacc agcggcaaca
     8761 ccggcatcgc gctggcgatg gtgtgcgcag cgcgcggcta ccgctgcgtg ctgacgatgc
     8821 cggaaaccat gagccgcgag cgccgcatga tcctgcgcgc ctacggcgcc gaactgatcc
     8881 tcacgccggg ccccgacggc atgggcggcg cgatcaggaa ggccgaggaa ctggtcgctt
     8941 ccgacaagcg ctacttcatg ccgcagcagt tcaagaaccc ggcgaacccg gaagtgcacc
     9001 gccgcaccac cgccgaagag atctggcgcg acaccgacgg ccgtgcggac atactcattt
     9061 ccggaatcgg caccggcggc accatcaccg gggttggcga agtgatcaag tcgcgcaagc
     9121 cggagttcag gtgcgtcgcg gtcgagccgg atgcctcgcc ggtgctctcc ggcgggtcca
     9181 aggggccgca cccgatccag gggatcggcg ccggcttcgt gccggacatt ctcaataccg
     9241 aggtctatga cgaaatcatc cgcgtcaaga acgaggatgc attcgcgttt gcccgccggg
     9301 cggcgcgtga ggaaggcctg ctggtcggga tctcctccgg cgcggccctg tgggcggcgc
     9361 tggaagtggc ccggcgcagc gagaacgccg gcaaactgat cgtcgtgatc atcccgtcgt
     9421 tcggcgaacg ctatctgagt acgccgctgt tcgccgacct ggcggactga aggcccgggt
     9481 ccgccggcga atgatgcaat cagatcaatc gaaaccgggg ctccgtgcct cgcgctgccc
     9541 gatcgaccgg cgctatgccg gcgatcggga ttttttatcc ccggttccgc aaccaaaccc
     9601 tcccttgcct gtcgtaaggg tgcttcgtcg aaacattgcg catggcaaat atcgcaagtc
     9661 gacgagcccg ccgctcttca gttcgggacg gcgggaaccc gaatcgacag gaacaggatg
     9721 ccaacatttt ccaaagtgtt tgaatctacc aagaacattt acgtccgcat cggcctcgtg
     9781 gccgtcgtcg cgctgatcgc gctcgtgatc gcgttccgcc cgatgtcgac ggcggagccg
     9841 accacggtcg tgcagtccca ggacattacc gagatcaccc gcctcgctgc ggaaaaggaa
     9901 aggctcgagt acctgctgct cgccaatccg ctctccaatt tcccccgcta cgtcttcaag
     9961 tacaaggacg caccgcagat ccatgtggtg aaggtgccga gcacctcgca ccagaacctg
    10021 gaacgcgaag tgctgctgaa gaacggcatt ccttacgcaa tcgcctcgga gcagttcctg
    10081 gagacccaca aggaggcgct gaaggacgaa gccacccggc gcgagaccat gtccgaagtc
    10141 ggcatgttca tcgcgcgcaa tgcggtcggc ctgctgctga tcggcttcct gatctatttc
    10201 ttccgcaagg gcgtgccggg catgagcgcc tcggcctccc ttatcaagcc ggagagcctc
    10261 aagggcagca tcgacgacct ggtcggcatg gaagacatca agcgcgaggt cgcgcacctg
    10321 gaagaaatga tccgcgaccg tgactcttat cgctcgcaca acatcgacaa gccgttcaac
    10381 gtaatgctga ccggcccggc cggcaccggc aagaccaagc tggccggcta cctggccaag
    10441 aagctgaaca ttccgctgat ccaggcttcc ggctcgggcc tcgaatccgg cttcgtcggc
    10501 ggcggcgcca aggcgctcaa cgcgctgtac aagaaagcct gcgcccagga acgctgcatc
    10561 atcttcctcg acgaggcgca gaccctgttc atgccgcgcg gccgcggcga caagaagtgg
    10621 gacgacgaca ccgccaacac cctgctcggc ctgctcgacg gcatcaagag caacgagggc
    10681 agcggcgtga tctggatcgt cgcgtcgaac ttcgacgatg ccagcatgca gatggacgag
    10741 gcgatgctgc gccgcttctc ggtgaagatc aacttccgcc tgccgaaccg ctcggagcgc
    10801 cgcgaactgg tgcgcgtgtt cctctcgcgc aaggatgacc gctgcgtgga ctggaacgac
    10861 ctgaacctgg attacgtggc cgaaatcacc gccaacctgt cgccggcact gatcgaaaac
    10921 gtggtcgacc gtgcctcgat gatcgcgatc caggaaaaga ccgtgatcaa caccgacgtg
    10981 ctgttccgtg ccttcgagcg cgccgcgatc ggcctgaccg accgcgcgac caccgccgac
    11041 aagaccaagg aacgcgagcg catcgcgctg cacgaactcg gccacttctt catgcagatc
    11101 gatccgtatc tcaagaaggg catgagctgg gccgaagtga aggaaaagtc gcacctgctg
    11161 aagatctcga ccgaagcggt ctccaagatc ggcgcgctgg gttacgtgct gtcttcgcag
    11221 gacgacgtgg cgctgcgcac gctggaagag ctggaacagg aagtgatcca cctgtacggc
    11281 ggcgtggcgg cggaagaact gttctacggc gcgcgcggca tttccaccgg cagccagaac
    11341 gacatccaga aggtcacggc cctgctcgac aacatggtca accgcctgtc gatgtacaag
    11401 cactcgaagc tggattaccg ccagctgaaa ggcgacgccg gccaggaaga cgcgatccgc
    11461 caggtcgagg cgaaggccga cgagctgtac gtgcgcacgc tcgaagcgac gaaggagtat
    11521 cgcgacctga tgtgggcgct gaaggacacc ttgctcgacc gctacgtgct gtccaaggac
    11581 gaggtgttcg cgctgctcga ggaaatgacg atggagcgcg aggagcaggc agcgtaagcg
    11641 gcatgcacat gaccggatga agggccgcag ggcgcaagct acgcggcctt ttttcttgca
    11701 ccggcgccac aagtcggccg cgggcccgcg accggaaaaa atcaagcaac acgacgaggc
    11761 gggaaactaa cgcgcccttc ccggcttcca ctacctgatt gccgctttca tatcgctttc
    11821 aggagatcac cgttgtcggt cccgcccgga acccgttcgc aggccggctc tgccatggcc
    11881 agcagtcgtg cgacgcggcc cgccctgtcc aaccccggca gccgcatgcc gcgatacgca
    11941 tgaggcggcg ccgcacgcct gcacggatcg cacgcctgac ccttcccggt ctcgccgcca
    12001 tgctgctcgc gtgcacgacg ccaccgctgt caacgccgga gattgcggcg tccctcccgg
    12061 acgcctatcg cagcggaagc gcggccggcc cgattctcga tgcctggtgg acccgcttca
    12121 acgacgctcg cctgaacgcg ctcgtgctgc atgcggcgca ggacagtccg tcggtcgccc
    12181 aagcggccgc gcgccttcgc atcgcgcaag cgcaggcgcg cgcagcgcgc gccgatttct
    12241 cgccgcaggt cagcgccggc ttcaacgcgg cgcgccagag gcagaacctg gccgggcttg
    12301 gccccatcgg cgagctgccg ctggcgccgg gcgacgcagc cggcgaatcc gccagcttca
    12361 ccaccagcaa cttcggcctc tcgctggaca tcagctggga gcttgacctg tggggccgca
    12421 tcgccgccca gagcgccgcg gcgcgcgcgg atttcctcgc cagccgggaa aacctgcgcg
    12481 cggtgcgcca gtcggtcgcc gcgcagacgg cgcggctgta tttcaccgtc acggaggcgc
    12541 gcgcccaggt cgacctggcc gagcgcaccg cggccacgat cggcgaaatc gcgcggcaga
    12601 tcaacaaccg tgccgacgcc gggatcgcgg caccgagcga tcgtctgctt gccaacgcaa
    12661 acctggaatc ggcgcgctcc ggcctgcagc agcgccgcga agcgctcgag cgcgcaacgc
    12721 gccagctgga ggtgctgctg cgcgactacc cagccggcgg catcgccacc gcgagcgtgc
    12781 tgcccgaggt gccgccacct gcgccggccg gtctgccggc ggacctgctc aagcgccggc
    12841 ctgacatcct ggccagcgaa ctggccctgc atgcggccgg ataccggctc accgcggccg
    12901 agcgcacgct gctgccgaac attgcgctga ccggctcggc cggcaccgcc agcgccgagc
    12961 tgggcaatct cctgagcggc ggctcggtgg tctggacgat cgcagggcgg gtgctgcagc
    13021 ccatcttcca ggccggccgg atacgcgcgc aggtggacat tgtcgaggga cagcgggcgg
    13081 aagcgatcga ggcctacgcc gacacggtcc tgcgcgcgct ggccgaggtg gaaaccgcgc
    13141 tcgcggtgga tacgctgctg gcgcagcgtg aatcggcact ggacgcggcc gccagcgcgg
    13201 ccgaaggcgc ggtggcgatc tcgttcaacc gctatcgcga agggatcgat ccgtttctcg
    13261 ccgtgctcga aagccagcag cgcgcgttgg acagccgcag cgcctatctc gcggcgcgcc
    13321 gcgcccggct cgaaaaccgg atcgacctgc acctcgcact gggcggcgga ttcgagccgg
    13381 caccggacgc aaaacaggaa tagatgatgt tgatcgacct gctccgatcc ctgttcccct
    13441 cctcgccttg cctgcgccgc gcaacgcaat ggagcagcga tgccaaacgt cgccaccctg
    13501 ccctgcagcg cgccgcccgg gcagctatgg tcgtcaccat tgcatgctgg agcaccgctt
    13561 gcggccgcgc cgacgcccag cagcccgcgc ctgcccagga agccggcgca gtcagcgtgc
    13621 gtgtcgtgcc ggccatgccg gccgccgtgc aggcctggct gtatggccag ggcaccgcac
    13681 gtgcgcaccg gcgcgagttc ctgagctttg ccaatcaggg gcgcgtgacc tatgtcgatc
    13741 cgaagctgcg cgtgggcagc cgggtgaaga agggccagct gatcgcctat caggaaccgg
    13801 accgcgtgcg cgccgatctc gcatcggcgc gggcgggtct ggcgggcgcc caggcggagc
    13861 ttgccgcggc gcgcgcggcg ctgagcgaag ccgaggccaa tctcgaactg gcacgcgtga
    13921 cccacgaacg ctatctggtg ctgatcgccc agaactccgc ctcccggcag gagctcgacc
    13981 aggcgcgcgc ccagctcgag caggcgcgtg cggcctaccg gcgggccgaa gtgcagatca
    14041 agtcatccga ggcacaggtc aatgcctcgc gttcccaggt cgcgcaggcg caggtgatcg
    14101 tgtccgagtc ccgtctggtg tcgccgatag acggggtgct ggcgcgcctg aatatcgagc
    14161 agggccgcta tttcacgccg caggtggtgc agaccgggac ggaacagggt gcgctgcgca
    14221 cggtgccggc ggtcgtgatc gatccgtcgg cgttcgagat caccgtcgac ctgcctgcct
    14281 atgcgttccg ccagatcgag gaaggcgcgg cggtggtcat cggccatgca cgcgcgcagg
    14341 cgccgccgac gataggcggc agcgcgggcg ggccacaggc ggcgcaggcg cagcaggcag
    14401 cgccggcccc ggaaggcgaa ctgcagggtc gcgtgcacgc ggtgagcccg tccctggacc
    14461 cggaaacacg caccttccag gtgatcatcc gcacgacgac cgaaacaccg cgcctgcagg
    14521 acggcgagtt cgtcaacgtc tggatcgctg gccaggcgcg tgaccaggtg ctcgccgtgc
    14581 cgctcgaagc gctgcgctat caggccggcc aggccttcct gttcgtcgtc gatccgcaga
    14641 gcggcattgc ccgcgaacgg cgcgtggcgc tcgggcagat cgaaggcaat cggcaggcgg
    14701 tgctctccgg attgtccgaa ggggagcttg tggtcaccga ggggcgcgca cggctcaatg
    14761 acgggcag
//
LOCUS       DATLGF010000301         5818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_228494, whole genome shotgun sequence.
ACCESSION   DATLGF010000301 DATLGF010000000
VERSION     DATLGF010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228494"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..157
                     /locus_tag="VM406_09205"
     CDS             <1..157
                     /locus_tag="VM406_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HVL76182.1"
                     /translation="VWLVHMRELWGITKTGGWALELQGSFLFAALAVALLGAGRFSIG
                     GASGRWN"
     gene            complement(308..1276)
                     /locus_tag="VM406_09210"
     CDS             complement(308..1276)
                     /locus_tag="VM406_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HVL76183.1"
                     /translation="MKIVTTMLALLSAALISACGGSDSDPPAESATAPGVAQPISQVA
                     ALQGFSSLLAAAEKAGLATALADPNASLTVFAPTDEAFNALATALGFQDAGAMVEALP
                     ASALAGVLQYHILPQRRSAADIGSAPRSEAATLYQFAGQAATLELDTTDGVRIEDAVL
                     AEARVTAADVAASNGVIHVIDKVLVPPGVLNIVQMAQLNPAFSTLVEAVTETGLQEAL
                     SGPGPFTVFAPVNDAFAAVPGPLAAEQLRTVLTYHVLNAEVGAADIPFGSPVTTLATQ
                     PITIDAGDPPTIADTSAVRATIVATDVRASNGVIHVIDKVLIPQLQ"
     gene            complement(1559..1849)
                     /locus_tag="VM406_09215"
     CDS             complement(1559..1849)
                     /locus_tag="VM406_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HVL76184.1"
                     /translation="MATAEGHIQDRRHIPDRRAHGSVHGRERAMSAIDWIAMVLLIVG
                     GLNWGLVGLFNFDLVAMIFGEMSMLSRIVYTLVGLSALYAIYTSSKMSKART"
     gene            2031..2963
                     /locus_tag="VM406_09220"
     CDS             2031..2963
                     /locus_tag="VM406_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HVL76185.1"
                     /translation="MTSPPCNALSIAAVERETGLSKDTLRVWERRYRFPRPGRDAYGE
                     RSYPPDQVEKLRLIRVLMDQGHRPGKIVGEDLDVLRRMAARGDNSVAAVPALPSRSGL
                     EHYIALCKAHRQEELRHALSQALLRQGMYSFVVEVIAPLNAVIGAQWAGGAMAVFEEH
                     LYTECVQAVLRSAVASMPARPAVSRQGARPRILLTTVPQEQHGLGLLMAEAIFALEGA
                     RCISLGTQTPISDIVRASAAHAADVVALSFSASFRPALLAQALADLRGGLPKQVEIWA
                     GGHNAALHRKAPQAVRVMELQDIGAAVTDWRQRH"
     gene            complement(3046..3381)
                     /locus_tag="VM406_09225"
     CDS             complement(3046..3381)
                     /locus_tag="VM406_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HVL76186.1"
                     /translation="MTFIDAIKTCFAKYADFTGRATRSEYWWFFLFVVLGSIAAGAIA
                     DVVHLLFMLATLLPSLAAAARRLHDTGRSGWLLLIGFIPIVGALVLIYFLVQPGEGAN
                     RFGEPLAVQ"
     gene            3661..5268
                     /gene="ctaD"
                     /locus_tag="VM406_09230"
     CDS             3661..5268
                     /gene="ctaD"
                     /locus_tag="VM406_09230"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HVL76187.1"
                     /translation="MANPTEGAAHPGMHGHEDEHAHHHPTGWRRWLFATNHKDIGTLY
                     LWFSLIMLMAGGLLAMFIRAELFQPGLQLMQPELYNQFVTLHGLIMVFGAIMPAFVGF
                     ANWMIPLQIGASDMAFARMNNFSFWLLPPAGILLTGSFFVGGGATAAGWTLYPPLAVQ
                     MGPGMDMAIFAVHIMGASSIMGSINIITTILNMRAPGMTLMKMPLFCWTWLITAYLLI
                     AVMPVLAGAITMLLTDRHFGTTFFNAAGGGDPVMFQHIFWFFGHPEVYIMILPAFGII
                     SQIIPAFSRKPLFGYASMVYATASIGILSFVVWAHHMFTTGMPVTGQLFFMYATMLIA
                     IPTGVKVFNWIATMWKGALTFETPMLFAVGFVFVFTIGGFSGLILAVAPIDIQVHDTY
                     YVIAHFHYVLVAGSLFALFAGFYYWAPKWTGYMYSEWRGKFHFWNSLIWLNVTFFPMH
                     FLGLAAMPRRYADYPAQYTDFNMLTSVGAFLFGLTQVYFLFFIVLPSIRGGVKAADKP
                     WEGAEGLEWTVPSPAPFHTFEVPPTVK"
     gene            5457..>5818
                     /locus_tag="VM406_09235"
     CDS             5457..>5818
                     /locus_tag="VM406_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL76188.1"
                     /translation="MTAELIRDTLRDQADIAFHYEPDASQTLARCRELQPTVVLVDLK
                     MPGIGGMEVLQQVRTCAATRLLPLILLSSEDAPEIKAEAFARGAHDYLVKWPAKRELV
                     ARLRYHSAAYWAHRERDEA"
BASE COUNT          956 a         1862 c         1897 g         1103 t
ORIGIN      
        1 ggtctggctc gtacacatgc gtgaactgtg ggggatcacg aagaccggcg gctgggcgct
       61 cgaactgcag ggctcgtttc tgttcgccgc gctcgcggtg gcgctgctcg gagcagggcg
      121 cttcagcatc ggcggcgcgt ccgggcgctg gaactgaggc ggcgtctccg ttccccccag
      181 gtttttcgag aggagcgcct ggtacggccc ctgctcatgt ccctcgcgtt cggcttccct
      241 ggccagctcg gccgccggcg cggcccgctt gctccgcgcg ccgcactccg tggctccccg
      301 tcgcggctca ttgcagctgc gggatcagga ccttgtcgat cacgtggatt acgccattgc
      361 tcgcgcgcac gtcggttgcg acgatcgtgg cgcgcacggc actcgtgtcc gcgatcgtcg
      421 gcgggtcgcc tgcgtcgatc gtgatcggct gcgtggccag cgtggtcacc ggtgagccga
      481 aggggatgtc ggccgcgccc acttcggcat tcagcacgtg gtaggtgagt acggtgcgaa
      541 gctgctcggc ggcgagcggg ccgggtacgg cagcgaacgc atcgttcacc ggtgcgaata
      601 ccgtgaacgg gccgggtcct gacaaggctt cctgcaggcc ggtttcagtc accgcttcca
      661 ccagcgtgga gaaggcggga ttgagttgcg ccatctgcac gatgttcagc actcccggcg
      721 gcaccagtac cttgtcgatc acatggatca cgccattgct tgcggccacg tcggccgcag
      781 tcacgcgcgc ctcggcgagc acggcgtcct cgatgcgcac gccgtccgtg gtgtcaagtt
      841 caagcgttgc cgcctgtccc gcgaactggt acagcgtggc cgcttcggat cgaggtgcgg
      901 agccgatgtc cgctgcactg cgtcgctgcg ggaggatgtg gtactgcagc actcctgcga
      961 gcgcgcttgc cggcaaggcc tccaccatcg cgccggcatc ttggaagccc agcgcggtgg
     1021 ccagcgcgtt gaacgcctcg tcggtcggcg caaacactgt gaggctggca ttcggatccg
     1081 cgagcgccgt ggccaggccg gccttttccg cggcggccag caaggaactg aaaccctgca
     1141 gcgccgccac ctggctgatc ggctgggcaa cgccaggggc cgttgctgat tccgccggcg
     1201 gatcactgtc gctgcctccg caggcactga tgagcgccgc agacagcagc gcgagcatgg
     1261 tcgtcacgat cttcacgtca aatctccttc gagtggtggg gcgcaaggag ctcgcttgcg
     1321 cgcattgacc cgccggctgg taccgaaagc ataggagtcg acctgttgaa gcgtttgatc
     1381 tcgcacaaac cgcaggagtt ttgtcttgga caaaaattga ggtcgttgtg tgccgcgacg
     1441 gttgtttgcc gggcgggccc gggcatgcgc cgaggaggga gggagcgaaa ggaggagacg
     1501 gacgaaaaaa agccggtgcg tcgggcaccg gctgcgccag gcgcgtattg cggtccggct
     1561 aggtgcgcgc cttcgacatc ttgctgctgg tatagatcgc gtacagcgcg ctcaggccga
     1621 ccaaggtgta gacgatgcgc gagagcatgc tcatctcgcc gaaaatcatc gccacgagat
     1681 cgaaattgaa cagcccgacg agcccccagt ttaggccccc cacgatcagc agcaccattg
     1741 cgatccagtc gattgcgctc atcgcgcgct cacgcccgtg aacggacccg tgcgcacgcc
     1801 ggtccgggat gtggcgccga tcctggatat gtccttctgc agttgccatg actacctcct
     1861 cgacagattg aacaaagccg cgcagatcca tgccgcgccg caacgcgcat cgccggaacc
     1921 atgcgcatgt acatgcccta gacagcggcg caaggtcgag gttctctata tttgtcttgg
     1981 acagggttcg caactagaat gagcttgtgc ccacccgagc cgagccgtga atgaccagcc
     2041 ctccctgcaa tgcactcagc atcgccgccg tcgagcgcga gacaggcctc tccaaggaca
     2101 ccttgcgcgt atgggagcgc cgctaccgct tcccgcggcc gggccgggac gcgtacggcg
     2161 aacggagcta tccgccggac caggtggaaa agctgcgatt gatccgcgtg ttgatggacc
     2221 agggccaccg tccgggcaag attgtcgggg aggacctgga cgttctgcgc cgcatggcgg
     2281 cgcgcggcga caacagcgtc gctgcggtgc cggcgctgcc gtcacgcagc gggctcgagc
     2341 attacatcgc tttgtgcaag gcgcatcgtc aggaggagtt gcggcacgcg ctgtcccagg
     2401 cattgctgcg ccaaggcatg tacagcttcg ttgtcgaggt gatcgccccg ctcaatgccg
     2461 tgatcggcgc gcagtgggcg ggcggcgcga tggcagtttt cgaggagcat ctgtacaccg
     2521 agtgcgtgca ggccgtgctg cggtcggcgg tcgcgtcgat gcccgcgcga cccgcggtct
     2581 cgcggcaggg tgcgcggccg cgcatcctgc tcaccacggt gcctcaggag cagcatggct
     2641 tgggtctgct gatggcggaa gcgatcttcg cgctcgaggg ggcacgctgc atttcgctcg
     2701 gtacccagac cccgatctcg gacattgtcc gcgcttccgc tgcgcatgcg gcggacgtcg
     2761 tggcactgtc gttttcagcg tcgttccggc cggccctgct tgcgcaagcg ctggcggact
     2821 tgcgcggggg gttgccgaag caggtggaaa tctgggccgg cggccacaac gcggctctgc
     2881 atcgcaaggc gccgcaagcc gtcagggtga tggagctgca ggatatcggc gctgcggtga
     2941 cggactggcg gcagagacac taggcttcgc agccggcagg gtgccggcgc taggggcgat
     3001 cgcgaaaagg aaaggcctgc agccgcgctt ccgatcgcgg tccggtcact ggaccgccaa
     3061 cggttcgccg aagcggtttg cgccttcgcc aggctgcacc aggaaataaa tcagtaccag
     3121 cgcgccgacg ataggaatga agccgatcag cagaagccag ccgctgcgac cggtgtcgtg
     3181 caggcggcgc gcggccgctg ccagcgaggg cagcagcgtg gcgagcatga acagcaggtg
     3241 gacgacatca gcgatggcgc ctgcggcgat actgccgagc acgacgaata gaaagaacca
     3301 ccagtactcc gagcgtgtgg ccctgccggt gaagtctgcg tacttcgcaa agcaggtctt
     3361 gatggcgtcg ataaaggtca tggtttccct cctgaagtgt tgttatcgtg gggcaaagat
     3421 agcacacaag caatacagcg ccgtggcacc tgcccgtgat ggcgaacact gcttcccatt
     3481 ccacgtcaag cctgatctac gcggctggaa ctctgtcccg cccgctcctg tcaaggcgga
     3541 ttcccacggc acgccgcacg cgtcccttgc tccccagatg tgccgctccc atcgggcgcg
     3601 tcgcaagcgt ttgcgcccgg tctgccggac ggttccccat ggcccgagac ggaggttccg
     3661 atggccaatc cgaccgaagg cgcagcgcac ccgggcatgc acggccacga ggacgaacac
     3721 gcccaccatc accccacggg ctggcgccgc tggctgtttg ccacgaatca caaggacatc
     3781 ggcacgctct acctctggtt ctcgctgatc atgctgatgg ccggcgggtt gctggcgatg
     3841 ttcatccgcg cagaactgtt ccagcccggc ctgcagttga tgcagccgga gctgtacaac
     3901 cagttcgtga cgctgcacgg cctgatcatg gtgttcggcg cgatcatgcc ggccttcgtc
     3961 ggcttcgcga actggatgat tccgctgcag atcggcgcct ccgacatggc gttcgcgcgc
     4021 atgaacaact tctcgttctg gctgctgccg ccggccggca tcctgctgac cggctcgttc
     4081 ttcgtcggcg gcggcgccac cgcggccggc tggacgctgt acccgccgct ggcggtccag
     4141 atggggccgg gcatggacat ggcgatcttc gccgtgcata tcatgggcgc ctcgtcgatc
     4201 atggggtcga tcaacatcat caccaccatc ctcaacatgc gcgcacccgg catgacgctg
     4261 atgaagatgc cgctgttctg ctggacctgg ctgatcaccg cctacctgct gatcgccgtg
     4321 atgccggtgc tggcaggcgc gatcacgatg ctgttgaccg accgccactt cggcaccacc
     4381 ttcttcaacg ccgccggcgg cggtgatccg gtgatgtttc agcacatctt ctggttcttc
     4441 ggtcaccccg aggtctacat catgatcctg ccggcgttcg ggatcatctc gcaaatcatc
     4501 ccggcgtttt cccgcaagcc gctgttcggc tacgcatcga tggtgtatgc gaccgcctcg
     4561 atcggcatcc tgtcgttcgt ggtttgggcg caccacatgt tcaccaccgg catgccggtg
     4621 accggtcagc tgttcttcat gtacgcgacg atgctgatcg caattccgac cggcgtgaag
     4681 gtgttcaact ggattgccac gatgtggaaa ggggcgctga ccttcgagac cccgatgctg
     4741 ttcgctgtcg gattcgtgtt cgtattcacg atcggcggct tttccgggct gatcctggcg
     4801 gtggcgccga tcgacatcca ggttcatgat acttactacg tgattgcgca tttccactat
     4861 gtgctggtgg ccggttcgct gttcgcgctg ttcgccggct tctactactg ggcgccgaag
     4921 tggaccggct acatgtacag cgagtggcga ggcaaattcc atttctggaa ttcgctgatc
     4981 tggctgaacg tgaccttctt cccgatgcac tttctcggcc tcgcggcgat gccgcgccgc
     5041 tatgccgact atccggccca gtacaccgac ttcaacatgc tgacctcggt cggcgccttc
     5101 ctgttcggcc tgacccaggt ttacttcctg ttcttcatcg tgctgccgtc gattcgtggc
     5161 ggcgtgaagg cggctgacaa gccgtgggaa ggtgcggaag gcctggagtg gaccgtgccg
     5221 agtccggcgc ctttccatac cttcgaggtg ccgccaacgg tgaagtaagg ctgttcgttt
     5281 gatatgcctc aaacagcgtt tcggtagccg tcgtttccgg gcgtcacgcg aattcacgcc
     5341 ggaaaggcac ggttagaatg cgcgcttgcc cgatgcgccc ccggaggcga tggaaacgtt
     5401 tgacgatgca tgcagcgaat cacacagtct cggtacttct tgttgacgat cagcacgtga
     5461 cggcggagct gatccgggac acgctgcgcg accaggccga tatcgctttc cactacgagc
     5521 cggatgcttc gcagacgctg gcgcgctgcc gcgagcttca gcccaccgtc gtgctggtgg
     5581 atctcaagat gcccggcatc ggcggcatgg aagtgctgca gcaggtgcgc acctgcgccg
     5641 ccacgcgcct gctgccgctg atcctgttgt cctccgaaga cgcgccggag atcaaggccg
     5701 aggcattcgc gcgtggcgcg cacgactatc tggtgaaatg gcccgccaag cgcgaactgg
     5761 tcgcgcgcct gcgctatcac tccgccgcct actgggcgca tcgcgaacgc gatgaggc
//
LOCUS       DATLGF010000302         4034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_228522, whole genome shotgun sequence.
ACCESSION   DATLGF010000302 DATLGF010000000
VERSION     DATLGF010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228522"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /gene="cpaB"
                     /locus_tag="VM406_09240"
     CDS             <1..337
                     /gene="cpaB"
                     /locus_tag="VM406_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HVL76189.1"
                     /translation="LAVAQEAGRDDTKPRVVSAVTLELSPQDAEKLDLARSVGTLSLV
                     LRNQIDNGPVTTAGATKQQLFGEKPAQPKSTARPVPVRTAARSKAPPEQSCVEVIHYG
                     TRVLNCFPR"
     gene            351..1928
                     /locus_tag="VM406_09245"
     CDS             351..1928
                     /locus_tag="VM406_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HVL76190.1"
                     /translation="MHVLNPFRSVALAAMLALSSLAAVGQESHGAATRLRPCTGVQVD
                     PPALVTLGKSTVISLDFPVVRMVVGGQSTSRAGQPADAADKEKSAAATPPRNEHGVAE
                     VDITLLNSTELFILGKQPGSMNIVLQGTQGRCAIKDIIVTLDPTTLQATLRELMPEEA
                     GVRIRAAEGALVLTGDVSDAGRLDEIVRIARSYGDGKKVVNMLRVAAPQQVMLEVKIA
                     EVSKTLLDKFGLDFSRLVTSDGGLTSRIVSGIVGGGAAAIGHYGGNLAPGYVTGRAVA
                     GVAGSSAAAAGALGHPEFGFPTSNGTLLGIDAEKRDGLVRVLAEPNIMAISGQKASFL
                     SGGKIFIPVAQDTVAGGTRITLEEKEFGVGLQFTPTVLGGRINLKLVSEVSELSQTGS
                     PFTAINGVTAILPSMTTRRADTTVQLGDGQSFVIAGLIRNNVTETINRFPGVGEVPIL
                     GALFRSTEFQNDQTELVFVVTPRLVKPLTGPVTLPTDNHVVPSRADVLFRGMAEGAAP
                     SPDTSGAPEPAVQPETK"
     gene            1939..2214
                     /locus_tag="VM406_09250"
     CDS             1939..2214
                     /locus_tag="VM406_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76191.1"
                     /translation="MKAITCISLAALLAGCATSTTPNYDARFGDAVREARARMTFDPQ
                     AGTKDHPVAGLDGPSAAEAVGRYQDTYKAPPPVTNVINIGGSISSGR"
     gene            2298..2507
                     /locus_tag="VM406_09255"
     CDS             2298..2507
                     /locus_tag="VM406_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HVL76192.1"
                     /translation="MNVTLQTLGNAIRTFFKDEDGAQVVEYALIIAVVSIALVIALQA
                     LTADGGGFATFIGRVTTCLTTATCT"
     gene            2609..2785
                     /locus_tag="VM406_09260"
     CDS             2609..2785
                     /locus_tag="VM406_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HVL76193.1"
                     /translation="MLAQFMRDEDGAQVVEYALVIAVVSIALVLALDALTIANSFPSF
                     IGRVATCLTTAQCS"
     gene            2855..>4034
                     /locus_tag="VM406_09265"
     CDS             2855..>4034
                     /locus_tag="VM406_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HVL76194.1"
                     /translation="MTTALVLLFLLGFSAIALDFGHLFVVKTELQNAMDSCALSAAQE
                     LDGREDALVRATNAGIAAGNLNRVDFQSVNWRGRPMVTGDDISFRDAAYLPTAVPADA
                     RYAQCQHTQPGVQLWLLQAMGAFSGATASYPNAQNVFALAVATRSSAQTTCPIPLALK
                     PKPGSAAPDYGFEVGEWVTLLMQQNAAQGGQIGWANLDGSNNAAQTAAELEGSCGTRV
                     GDTLGTPGVQTSIADVWNYRFGIYKNTGDPAVNRPDFTGYAYTSNNWPSRRSAYDGTP
                     GPGAHATAQNFVTKRLAYASCADTGTRVRGANSCESITGLSLNSFQNLAAPGNSTGGH
                     RDYGSNRRLATVPVVNGANQVIDYACMLLLQPLSIPMTNVELEYRGNAGDPGSPCTTN
                     G"
BASE COUNT          762 a         1305 c         1281 g          686 t
ORIGIN      
        1 gctcgcggtg gcgcaggagg ccggccgcga cgacaccaag ccgcgagtgg tcagcgcggt
       61 cacgctggaa ctgtcgccgc aggacgccga gaagctggat ctggcccgca gcgtcggcac
      121 cttgtcgctc gtgctgcgca atcagatcga taacggaccg gtgacgaccg ccggcgccac
      181 caagcagcag ctcttcggag agaagcccgc ccaaccgaaa agcacggcac gacccgtgcc
      241 ggtcaggact gccgcgcgca gcaaggcgcc gcccgagcag tcctgcgtgg aggtgatcca
      301 ctacggcacg cgcgtgctca attgcttccc gcgataacgc aacaaggagt atgcacgtgc
      361 tgaatccgtt tcgttccgtc gcacttgccg ccatgctggc gctctcgagt ctggccgccg
      421 tcgggcagga aagtcacggc gctgccacgc gactgcgtcc ctgcaccggt gtgcaggtcg
      481 acccgccggc gctggtcacg ctgggcaaat cgacggtcat cagccttgac ttcccggtcg
      541 tgcgcatggt ggtgggcgga cagtccacca gcagggcggg ccagccggcc gatgctgccg
      601 acaaggaaaa gagcgccgcg gcaacgccgc cgcgcaatga acacggcgtg gcggaggtcg
      661 acatcacact gctcaattcg accgagctct tcatactcgg caagcagccg ggatcgatga
      721 acatcgttct acagggaaca caaggtcgtt gcgcgatcaa ggacatcatc gtcacgctcg
      781 acccgaccac cctgcaggcg accttgcgtg agctcatgcc ggaggaagcc ggcgtgcgca
      841 tccgtgcggc ggagggcgca ctggtgttga ccggtgatgt ctcggacgcg ggcaggctcg
      901 acgaaatcgt ccgcatcgcg cgctcgtacg gcgacggaaa gaaggtcgtc aacatgctgc
      961 gtgtggcggc gccacagcag gtgatgctgg aggtgaagat tgccgaggtc agcaaaacac
     1021 tgctcgacaa attcgggctg gacttctccc ggctggtcac ttcggatggt ggcctgactt
     1081 cccgcatcgt ctccggcatc gtgggcggcg gcgccgcggc gatcggccac tacggcggca
     1141 atctggcgcc cggctatgtc accgggcggg cagtagccgg tgttgccggc agttccgccg
     1201 ctgcggccgg cgcactcggg catccggagt tcggattccc gaccagcaac ggcaccttgc
     1261 ttggtatcga cgcggaaaag cgcgacggcc tggtgcgggt actggctgaa cccaacatca
     1321 tggcgatcag cgggcagaag gccagcttcc tttccggtgg caagatattc atcccggtcg
     1381 cccaggacac ggttgccggt ggcacccgca tcacgctgga agaaaaggaa ttcggcgtcg
     1441 ggttgcagtt cacgccgacg gtgctcggcg gccggatcaa cctcaagctg gtatcggaag
     1501 tctccgagct gtcccagacc ggttcaccgt tcacggcaat caatggcgtg accgcgatcc
     1561 tgccttcgat gacgacacgg cgcgccgaca ccactgtcca gcttggcgat gggcagagct
     1621 tcgtcatagc cggcctgatc cgcaacaacg ttacggagac catcaaccgt tttccgggtg
     1681 taggcgaagt acctattctg ggcgcgctgt tccgttcgac cgagttccag aacgatcaga
     1741 cagaactcgt gttcgtcgtc accccgcgcc tggtcaagcc tctgactggg ccggtcacgc
     1801 tgcccaccga caatcacgtc gtcccgagcc gcgcggatgt gctgttccgg gggatggccg
     1861 agggcgccgc gccatctccc gacacatccg gtgcacctga accagcggtc caacctgaaa
     1921 cgaagtaatg gagtggcgat gaaggcaatc acctgtattt ccctggctgc cctgctggcc
     1981 ggatgtgcga cctccaccac cccgaactac gacgcaaggt ttggcgatgc ggtgcgcgag
     2041 gcacgtgccc gcatgacgtt cgacccgcaa gcgggcacga aggatcatcc cgtggcaggt
     2101 ctggacggcc cgtcggcagc agaggcggtg ggccgctacc aggatacata caaggcgccg
     2161 ccgccggtca ccaatgtgat caacatcggc ggcagcatca gcagcggccg ctagccgcgc
     2221 caggtttgca gttcgcagtg gcagtaccca tgcagtaggc agcattcaac ctaaacccac
     2281 gatatgaggt gaaacacatg aacgtgactt tgcaaaccct tggaaacgcg atccgaactt
     2341 tcttcaagga tgaggatggc gcgcaggtcg tcgagtatgc actgatcatt gcggtggtat
     2401 cgatcgcgct cgtcatcgca ctgcaggcgc tgacagccga cggcggcggc tttgccacct
     2461 tcatcggtcg cgtgaccacc tgccttacga cggccacctg cacctgacgg gcgggtgatt
     2521 cggatcggtg gccttgcgaa cgcgcgaggc caccttctgc tcgccccgat gcggtcggac
     2581 ggccgcgggg cttaccggga gaatcgcgat gcttgctcag ttcatgcggg acgaagatgg
     2641 agcgcaggtc gtcgagtacg ccctcgtgat cgccgtggtg tcgatcgcgc tggtactggc
     2701 gctggatgcg ctcactatcg ccaacagctt cccttcgttc atcggccgtg tcgcaacctg
     2761 cctcacgaca gcacagtgca gctagaccct tcccatgcgt acgcaagttc tccttcccgc
     2821 atcgaagcga ggccggcagt gcggtgccgt catcgtgacc actgcgctgg tgctgctgtt
     2881 tcttctcggc ttttcggcga ttgcgcttga tttcggccat ctgttcgtcg tcaagacgga
     2941 gttgcagaac gcaatggaca gttgcgcgct ttccgccgcc caggaactcg atgggcgcga
     3001 agacgcgctg gtgcgtgcga ccaatgcggg cattgccgcc ggcaatctca accgggtcga
     3061 cttccagtca gtgaactggc gcggccgccc gatggtgacc ggagacgaca tttcctttcg
     3121 cgacgcggct tacctgccca ccgcagtgcc cgcggatgcg cgatatgcgc agtgccagca
     3181 cacccagccg ggcgttcagc tgtggctgct gcaggccatg ggcgcgttca gcggcgccac
     3241 tgcaagctat ccgaatgcac agaacgtatt tgctctcgcc gttgcaacac gatcgagtgc
     3301 ccagaccact tgtccgattc cactcgcgct caagccgaag cccggcagcg cggcaccgga
     3361 ctatgggttc gaagtgggtg aatgggtcac cctgctgatg cagcagaacg ctgcccaggg
     3421 cggacagatc ggttgggcga acctggacgg ttcaaacaat gcggcgcaaa cggcagcgga
     3481 gctcgagggt tcctgcggca cgcgcgtcgg cgacaccctg ggtacaccag gggtgcagac
     3541 atcgattgcg gacgtctgga actatcgctt cggcatctac aagaacaccg gcgatcctgc
     3601 agtgaaccgg ccggacttca ccggttatgc ctacacgtcg aacaactggc caagccggcg
     3661 cagcgcctac gacgggactc ccggaccggg agctcatgcg acggcgcaaa acttcgtcac
     3721 gaaacggctc gcctatgcct cctgcgccga caccggaacg cgggtgcgcg gcgcgaacag
     3781 ctgcgagtcg attaccggac tgagcctgaa cagcttccag aacctcgccg cgccgggcaa
     3841 cagcaccgga ggccaccgcg attacggcag caatcggcga ttggccaccg tcccggtggt
     3901 caatggggca aaccaggtca tcgactatgc atgcatgctg ctgctccagc cgctcagcat
     3961 tccgatgacc aacgtcgaac tcgagtaccg gggcaatgcc ggtgacccgg gcagcccctg
     4021 caccacgaat ggac
//
LOCUS       DATLGF010000303         3538 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_119138, whole genome shotgun sequence.
ACCESSION   DATLGF010000303 DATLGF010000000
VERSION     DATLGF010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119138"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="VM406_09270"
     CDS             complement(<1..1045)
                     /locus_tag="VM406_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HVL76195.1"
                     /translation="MSEAETARGLDRTRRLATGLLIAMAVVFVIARSQQAAYPWLSFV
                     AAFAEAAMVGALADWFAVTALFRSPFGLPIPHTAIIPRNKDRIGDSLSNFLEHNFMTQ
                     EIIREELRMIDFAGAVAGWLAQPENSRSLSRQVLRALPALLRVVEDEDVRQFMQQRLS
                     RAIDNTKLAPLAAELLSVLVASRHHQALFDRIVEMAARGLEQNRDYIRWKVHENSPRW
                     LPKAIDERFYRRLVEALESTLAEMRDEDSEWRLRFQHLVEDFIEQLRHSPEYEERMHV
                     AVNEVLGHPLVRDYTIDVWHELKKRIEAEAQAQDSPLGAKLDEALRSLGSELLADMRL
                     RRKLNQSIRILATE"
     gene            1218..1736
                     /locus_tag="VM406_09275"
     CDS             1218..1736
                     /locus_tag="VM406_09275"
                     /inference="COORDINATES: protein motif:HMM:NF045536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070088 - polyhydroxyalkanoate granule
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate granule-associated phasin"
                     /protein_id="HVL76196.1"
                     /translation="MNHITRRRRRSLPPAAVPPPASFAPWIQLSWKSAAMLGASAFVI
                     NHRVRRIAAAGNRPDASDQREFQRMGLEKIEAAWESAGAVGLRMMMLLPQAGMQMMQY
                     WMKLSAPPLAAGLFTPTSLAASQQAWLNGSITAWSELAKQQASAMSTLMSHGLKPIHR
                     RATANARRLWYA"
     gene            complement(1825..1915)
                     /locus_tag="VM406_09280"
     tRNA            complement(1825..1915)
                     /locus_tag="VM406_09280"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1879..1881),aa:Ser,seq:cga)
     gene            2247..>3538
                     /locus_tag="VM406_09285"
     CDS             2247..>3538
                     /locus_tag="VM406_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVL76197.1"
                     /translation="MLLARLHDILEHRQLNALFQPIIDVRNGEIVGYEGLIRGPSDSP
                     LHSPMNLFKTARAHGRSVQLEHLCRRVVLERFAELALPGRLFLNVSPEMLLQPHARRG
                     ETLAYIEQVGIQPERVIIELTENEPTYDYELLREAVRHYRGMGFRIAIDDLGEGFSSL
                     RLWSELRPEYVKIDMHFVQGINHDPVKLQFVRSIQEIAEQSGTAVIAEGIETQAELLL
                     IRDLGIRCGQGYHIARPHPNPPGSLANDVLQGLAGGGVPVYPHSHSPAHNAATAQKLL
                     RTVPVARPHMTNNDVCELFNDNPDLATIPVVADEQPVGLISRRDLVDRFARLYLRELY
                     GRKSCQTFMDPAPLIVDVNTGVQALSQMVVDGARHHLSNGFIITEHGRYLGMGTGHDL
                     MREITQMQINAARYANPLTLLPGSVPIDEHIDRLLRNNA"
BASE COUNT          652 a         1170 c         1092 g          624 t
ORIGIN      
        1 cttcggtggc gaggatgcgg atcgactggt tcagcttgcg gcgcagccgc atgtccgcca
       61 gcagctcgct gccgagcgag cgcagcgcct cgtccagctt cgcgcccagc ggtgaatcct
      121 gtgcctgggc ctcggcctcg atgcgtttct tcaactcgtg ccagacatcg atggtgtagt
      181 cgcgtaccag cggatggccg agtacctcgt tgactgcgac atgcatgcgc tcctcgtatt
      241 cgggcgaatg gcgcagctgc tcgatgaagt cctcgaccag atgctggaag cgcagccgcc
      301 actcgctgtc ctcgtcgcgc atttcggcca acgtgctttc gagcgcctcg accaggcggc
      361 ggtagaaccg ctcgtcgatc gccttgggca gccagcgcgg actgttttcg tgcactttcc
      421 agcggatgta gtcgcgattc tgctccaggc cgcgcgcggc catctcgacg atgcggtcga
      481 acagcgcctg atgatgccgg ctggcgacca gcaccgacag cagctcggcc gccagcggcg
      541 cgagcttcgt gttatcgatt gcccgcgaca ggcgctgttg catgaactgt cgcacgtcct
      601 cgtcctcaac cacgcgcagc aatgccggca gcgcacgcaa gacctggcgc gacaacgacc
      661 gactgttctc cggctgcgcc agccagccgg ccacggcgcc ggcgaagtcg atcatgcgca
      721 attcctcgcg gatgatttcc tgcgtcatga aattgtgttc gagaaaattc gacaggctgt
      781 cgccgatgcg atccttgttg cgcggaatga tcgcggtatg gggaatcggc aagccgaacg
      841 gcgaacggaa cagcgcggtc accgcgaacc agtctgccag ggcgccgacc atcgccgctt
      901 ccgcgaacgc cgccacaaag gaaagccaag ggtaggccgc ctgctgcgag cgcgcgatca
      961 cgaacaccac ggccatggct atcagcaagc cggtggcgag gcgccgggtg cgatccaggc
     1021 cgcgtgcggt ttcagcttcg gacatcgggg cgtgataagg taagggtatc ggttcgaacc
     1081 ggacaggccg gccttggttc gccgcttcaa catccccgcc tgggacggat gtcatgcagt
     1141 tggcatgaat caatccatat ccttggggca cgcatgcggc acaagaacca acggtagcag
     1201 gcgccgctcc aaccgagatg aaccacatca ctcgccgcag gcgccgttcc ctgccaccgg
     1261 ctgccgtccc gccgcccgcc tcattcgctc cctggataca gctttcctgg aaaagtgcag
     1321 caatgctggg agcatcggcc ttcgtcatca accaccgtgt gcggcgcatc gcggcggctg
     1381 gaaaccggcc tgatgccagc gaccagcgcg aattccagag gatgggtctg gaaaaaattg
     1441 aagcggcctg ggagtccgcc ggcgcggtag gcctgcggat gatgatgctc ctgccccagg
     1501 ccggcatgca gatgatgcag tactggatga agctctccgc gccgccgctt gcggcagggc
     1561 ttttcacccc cacgagcctg gccgcctccc agcaggcgtg gctgaacggg tcgatcacgg
     1621 cgtggtcgga actcgcgaag cagcaagcgt cggcaatgag caccctgatg tcgcacggcc
     1681 tgaagccgat ccatcgccgc gcaactgcga acgcgcgtcg gctttggtat gcctgaggcg
     1741 ccgaatcgga aaacaaaaaa cccgcgccgt ctcgaggact tgcgcgggct tttggacttc
     1801 cttgaacctc tcggttctca attctggcgg aaagggtggg attcgaaccc acgataggga
     1861 gttaccctat actggatttc gagtccagcg cattcgacca ctctgccacc tttcctgctt
     1921 ggtcgttgcg gcggggtgcg ccgcaaagca cgggattata gcagaagttg cttcgatggg
     1981 aagccccctc ttggctcgag caggacgcgc agcgtgctgc tgcgtcatcg gcctgtcata
     2041 tacagcggct agcatccgaa cgtcccgtcg caaaagaaac attccttgaa cacacatccc
     2101 ctgccagccc gccgcttgaa cacgtgcgtc cggagctgcg atccatgacg ctgcatttcg
     2161 ccgaagcgct cctgccgccg gtggccgcgc gctccctgat gcacctgccg aaaatccgtc
     2221 catccgatga ggagaagctc gacgtcatgt tgctcgcccg cctgcacgac attctcgaac
     2281 accggcagtt gaacgcgctg ttccagccga tcatcgacgt acggaacggc gagatcgtcg
     2341 gctatgaagg cctgatccgg gggccgtcgg acagtccgct gcactcgccg atgaacctgt
     2401 tcaagacggc gcgcgcgcat ggccggtcgg tgcagctcga gcacctgtgc cggcgcgtgg
     2461 tgctggaacg cttcgccgaa ctcgccctcc ccggccggct gttcctgaac gtgagtccgg
     2521 agatgctgct gcaaccgcat gcgcggcgcg gagaaaccct cgcctatatc gagcaggttg
     2581 gaatccaacc ggaacgcgtg atcatcgagc tgacggaaaa cgaaccgacc tacgactacg
     2641 agctgcttcg cgaggcggtt cgtcattacc gcggcatggg gttccgcatc gcgatcgatg
     2701 acctcggcga ggggttttcc agcctgcgcc tgtggtcgga gctgcggccg gagtatgtga
     2761 agatcgacat gcacttcgtc caggggatca atcacgaccc ggtcaagttg cagttcgtgc
     2821 gctcgatcca ggagatcgcg gagcaatccg gcaccgccgt gattgccgaa ggcatcgaga
     2881 cccaggccga gttgctgctg atccgcgacc tcggcatccg ctgcggacag ggctaccata
     2941 tcgcacgccc ccatccgaac ccgcccggca gtctcgcgaa cgacgtgctg caaggtctgg
     3001 ccggcggcgg cgtgcccgtg tatccacatt cgcattcgcc cgcgcacaat gcggcgaccg
     3061 cgcagaagct cttgcgcacc gtgccggtcg cgcgtccgca catgacgaac aacgatgtgt
     3121 gcgaactgtt caatgacaat cccgacctgg cgaccattcc cgtggtggcc gacgaacagc
     3181 cggtcggcct gatctcccgc cgcgatctgg tcgaccgttt cgcgcgtctc tacctgcgcg
     3241 aactgtacgg gcgcaagagc tgccagacct tcatggaccc ggcgccgctg atcgtcgacg
     3301 tcaacaccgg cgtgcaggcc ctgtcgcaga tggtggtcga cggcgcccgg catcacctgt
     3361 cgaacggctt catcatcacc gagcacggcc gctatctcgg catgggcacc ggccacgacc
     3421 tgatgcgcga gatcacgcag atgcagatca acgcggcgcg ctatgcgaat ccgctcacgc
     3481 tgttgcccgg cagcgtgccg atcgacgaac acatcgaccg gctgctgcgc aacaacgc
//
LOCUS       DATLGF010000304        17591 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_72188,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000304 DATLGF010000000
VERSION     DATLGF010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 17591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17591
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72188"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..806
                     /locus_tag="VM406_09290"
     CDS             198..806
                     /locus_tag="VM406_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76198.1"
                     /translation="MHPMMKGALAGAVATIPMTIVMSGLFRRLPREQQYPLPPVLITS
                     RVARHGDGEPALQGGRLTAAALAAHFAYGALTGAMYPLLERTRALDGPPALTGPAYGL
                     AVWAASYLGWIPAARILTPATRHPTQRNALMWTAHAVWGAALAAVCGLLQQREPASQE
                     LQTAWGTRAGNLDRPEYPGNKQGRMQPRERLHPAHQADARIS"
     gene            complement(799..2877)
                     /locus_tag="VM406_09295"
     CDS             complement(799..2877)
                     /locus_tag="VM406_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HVL76199.1"
                     /translation="MTAEYKVHGSVAVITLNNPPVNGLGHATRSAVVDGLRQAMNDEA
                     VKAIVITGAGKAFSGGADIREFNSPKALAEPTLHTVISAVENATKPVIAAIHTVCMGG
                     GLELALGCHYRVAAPGAQIALPEVKLGILPGAGGTQRLPRVVGLELALNMIVSGNPVP
                     SEKLAKTALFNLMAEGDLLEAAIAFANEVADARPLPKVRDQKIDYPNYEGFLQFSRNT
                     VRAMAGPFPAPLKCVDAVEAAITKKFDEGIAYERSLFLELVQTTESKALRHAFFGERA
                     ASKIADVPEDTPKREIKTAAVIGAGTMGGGIAMNFANAGIPVKILEMKQEALDRGLAT
                     IRKNYENTMKKGKLSPEKFEQRTGLISGTLSYDDIAQADIVIEAVFEEMGVKEQVFRK
                     LDEVMKPGAILASNTSTLDVNKIASFTKRPQDVIGLHFFSPANVMKLLEIVRGDKTGK
                     DVLATAMALAKKIKKTGVVSGVCDGFIGNRMIEQYSRQAGFLLEEGCLPEQVDKAIEK
                     FGFAMGPFRMGDLAGNDIGWAIRKRRYVEKPEVTYSKTADLLCEMGRFGQKTSAGWYD
                     YKPGDRKPYPSQPVNEMIVKHSADLGIERRKISDEEIVQRLVFALVNEGAYILEEGIA
                     QRASDIDMVYLTGYGFPLHRGGPMFYADTVGLQNVVMAMERFAKGRHGEAWKPAPLLA
                     RLAAEGKTFN"
     gene            complement(3246..4448)
                     /locus_tag="VM406_09300"
     CDS             complement(3246..4448)
                     /locus_tag="VM406_09300"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL76200.1"
                     /translation="MRIANLRSAFVLCAALPFLALAQHAEPIRIGVSGPFTGGSAPMG
                     LSMLNGIRLAVDEINGIGGVNGRRIELVERDDQANNDLGAKIADELTQMRVVATIGIV
                     NTGVGLASIDAYQKARIPLMIAVSTGTTLTRKYAPPHAPENFIFRVSPTLDLEARVVA
                     ADLKRKGLGSAAILADATAYGEAGLQAFLREAKAAGVEVAAQERFRIGDQDMSEQVRR
                     ARASNARALVVWGIGPEMAAIARNREAIGWNVPMLGSWTFSMLNFIEPAGRAGEGALM
                     PQTFIQDVGSSAKNSFLLAYRRAYNTDLIPSPMSAAQGYDGMHLLYLALRQANSTEGP
                     RIKEALEDLRMRYQGVITSYHRPFSREDHDAITQNMLLMGVVAKGRVDYAYADDQRRG
                     ALLRMKAN"
     gene            complement(4675..6327)
                     /locus_tag="VM406_09305"
     CDS             complement(4675..6327)
                     /locus_tag="VM406_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(methylthio)propionyl-CoA ligase"
                     /protein_id="HVL76201.1"
                     /translation="MTEYPKSPLMGQMMSQPLLIASIIRHADRHFGTNEIVSRRVEGD
                     IHRYTYRDCHRRARQMANALAAHGVKMGDRVATLAWNGYRHMELYYAISGSGAVMHTI
                     NPRLHPEQIAYIANHAEDQLLFFDLTFLPLVEAFASHCKTVRGYVLMCDRERMPAESK
                     LSGLMCYEDLVDGHSDQYEWPLFDENSASSLCYTSGTTGNPKGALYSHRSTILHSYGA
                     SMPDAMNISARDVVLPVVPMFHVNAWGLPYSAPLNGAKLVFPGPALDGKSIYELFEQE
                     KVTFSAGVPTVWLGLLNHVGENKLRFSSFRRTIIGGSACPPAMMKAFREQYGVEVIHA
                     WGMTEMSPLGTTCTLQSHHVSLAPEQQQAILEKQGHVIYGVDMKIVDDVGAELPWDGK
                     TYGNLLVKGPWVIGSYFKGEGGDVLQDGWFPTGDVATISPDGYMQITDRSKDVIKSGG
                     EWIGSIDLENIAVAHPAVQQAACVGVRHPKWDERPLLVVVKKPGAELKKEELLAFYEG
                     KIAKWWMPDDVVFVDALPIGATGKVLKNRIREQFKDHRLPTA"
     gene            6598..8097
                     /locus_tag="VM406_09310"
     CDS             6598..8097
                     /locus_tag="VM406_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HVL76202.1"
                     /translation="MNEIRPVAAPYRLDSLPLQNGFAALPPAFYTRLQPTPIPAPYLV
                     GASAAAARLIGLDASEFAHEEFLETFAGNRLLPGSQPLAAVYSGHQFGVWAGQLGDGR
                     AILLGDAAHAQGAGRLELQLKGSGLTPYSRMGDGRAVLRSSIREFLCSEAMAGLGIPT
                     SRALCVVGSDMPVIRETVETAAVATRMAPSFVRFGSFEHWFYNERQHELKLLADYVID
                     NFYPALRDADNPYRALLGEVTRRTAELIAQWQAVGFMHGVMNTDNMSILGITIDYGPF
                     GFMEAFDVRHICNHSDRQGRYAYQMQPRIGQWNCYALGQALLPLVGSVEEAQDTLEIY
                     KPAYDDKLDALLHAKLGLREKMEEDGELIDAMFALMQANHVDFTLFFRRLADLGRDPG
                     PGDEGVRDLVIDRAAFDAWAVKYRQRLARENSNDAERRAAMNRVNPKYVLRNYLAQTA
                     IEKAQDKDISEVARLLAILERPFDEQPEHEHYAALPPDWASHLEVSCSS"
     gene            8094..8507
                     /gene="msrB"
                     /locus_tag="VM406_09315"
     CDS             8094..8507
                     /gene="msrB"
                     /locus_tag="VM406_09315"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HVL76203.1"
                     /translation="MSEKVVKSDAEWRAQLEPMEYEVTRHAATERAFTGRYWDHHEAG
                     IYRCVCCGTALFASDTKFDSGCGWPSYFEPIDPANVIERIDRSHGMIRTEILCAICDA
                     HLGHVFPDGPPPTGLRYCINSAALQFEPARTASSA"
     gene            8526..9143
                     /locus_tag="VM406_09320"
     CDS             8526..9143
                     /locus_tag="VM406_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HVL76204.1"
                     /translation="MKFLFDLFPVILFFAMFKWGESNPEAALALSDRYLSALIAGGGA
                     TVEQAPILLATAVAILATAAQILYLVARGKKVESMLWISLAIIAVFGGATIYFHNETF
                     IMWKPTVLYWLFAGVLLGAQALFGRNLIRQMLEKQVALPGPVWHRLNLSWAAFFVAMG
                     LVNLYVAYSFSQSTWVNFKMFGFTGLMLAFVIAQSMMLSKYIKEE"
     gene            9151..9426
                     /locus_tag="VM406_09325"
     CDS             9151..9426
                     /locus_tag="VM406_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HVL76205.1"
                     /translation="MSARIEKMRSLLAATFTPLDCQLVDESHLHAGHAGAAGGAGHYR
                     LHIVSERFEGLNRLARHRLVYDCLRDMMPGEIHALNIAAVAPSELNR"
     gene            9508..10287
                     /locus_tag="VM406_09330"
     CDS             9508..10287
                     /locus_tag="VM406_09330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HVL76206.1"
                     /translation="MTFVQPARLLMALVAAIALPAMAQNLAVVNGKPIPSSRADTMIK
                     QLQAQGQPDSPELRAMVREELINREILMQEANRRGVTKNPDVQNQAEIARQSIAIRAL
                     VQDYLEKNPVSDAEIKAEYDKFKAQSGDKEYHARHILVETEEEAKGIINKLKGGAKFE
                     ELAKQSKDPGSAAAGGDLDWAPPGAFVKPFSDAMVSLQKGQFTQAPVQTQYGYHVIKL
                     EDVRDAQLPSLEEVKPHIAESLQQQKLQGFQEELRKKAKIQ"
     gene            10343..11113
                     /locus_tag="VM406_09335"
     CDS             10343..11113
                     /locus_tag="VM406_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76207.1"
                     /translation="MSPHPSIEHAADLETVVDARHPVRPVIWRGRSGFAKRPASLRSH
                     ERILLALAPLIRRWLGKDLPFHPVRYEKGWPVEVQRLRRLRAAGCAVPEVLHAERDLF
                     VSSDAGPTLAQLLADQVDAGVRLQWLNEAARDLAAFHQAGHWHGGAQVRNLVRTPEGT
                     LGRIDFETALDARFPLALLQAFDAALFFSSIVRSRESAALPDIARAYLDDAPEDARRV
                     LRRGLPLLRRMARSKLLRKLAPKEAERLRSIAALPLDG"
     gene            complement(11210..15223)
                     /gene="purL"
                     /locus_tag="VM406_09340"
                     /gene_synonym="purI"
     CDS             complement(11210..15223)
                     /gene="purL"
                     /locus_tag="VM406_09340"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HVL76208.1"
                     /translation="MLILPGSNALSAFRTQRLLSQLQAVEPAIVGITGRYLHFVDAPR
                     EPSAMDLERLAGLLNYGEPFAGNSEGEQFVVIPRFGTISPWASKATDIVHNCGMTHIH
                     RVERGVLYAVQMKSGLLGGAKKLSAEAVAAVTGLLHDRMVETVLRDANEAAGLFHTLE
                     ARPLEFVDVQKGGRDALVRANSELGLALSEDEIDYLFDAFTQARRNPTDVELMMFAQA
                     NSEHCRHKIFNADWIIDGEKQDKSLFAMIKNTHQQNPKGTIVAYSDNASVIEGATVSR
                     FYPRGEGRVYGASEELTHILMKVETHNHPTAIAPFPGASTGAGGEIRDEGATGRGAKP
                     KAGLTGFTVSNLMLTHAVQPWENARDVAEPVAQRAPHAQAGIYGKPERIASPLQIMIE
                     GPIGGAAFNNEFGRPNLGGYFRSYEQNVGGKVYGYHKPIMIAGGIGNISAKHTKKHDL
                     PVGSLLIQLGGPGMRIGMGGGAASSMATGTNTADLDFDSVQRGNPEMERRAQEVINAC
                     WALGDDNPVLSIHDVGAGGLSNAFPEITNDAKRGAVFDLRKVPLEESGLAPKEIWSNE
                     SQERYVLAIAPASLELFRAMCERERCPFAVVGVATEERQLKVIDPEHDNYPVDMPMDV
                     LLGKPPKMLRDVSHVHTDYAPVDLTGVDLLEAAQRVLRLPTVADKSFLITIGDRTVGA
                     TTVRDQMVGPWQVPVADCAVTAMSFEGYRGEAMAMGERTPLAAIDAAASGRIAVGEAI
                     TNIAAAPIADISDIKLSANWMAACGQPGQDAALFDTVKAVGMELCPALGISIPVGKDS
                     LSMRTTWNDAQGSKEVISPVSLIVSAFAPVPDVRRALTPQLRTDLGDTALILIDLGRG
                     KNRMGASALAQVMQQIGNEVPDVDDPQDLKNFFAAIQQLNAEGRLLAYHDRSDGGLFA
                     TLCEMAFAGHCGVSVSVDILAMEGEHASDSGDAKNWATQVGERRNEMSLRALFNEELG
                     AVIQVRADEKSEVMNVLRSHNLGACSHIIGKPNARGVIELMRDAKMVFSQTRVDLQRA
                     WSETSWRIARLRDNPTCADAEYDRLLDEKDPGLSPRLAFDPQEDIAAPFIATGARPRV
                     AILREQGVNSHVETAWVMHKSGFEAVDVHMSDLIAGRAKLDDFKGFIAVGGFSYGDVL
                     GAGEGWAKTILFNAALSDQFSGFFKRADTFALGICNGCQMLSNLKSIIPGAEAWPKFT
                     RNKSEQFEARFAMVEVAESPSIFFAGMAGTQSPIAVAHGEGYADFSRTGNIDEAIVAM
                     RWIDNRAQVTETYPFNPNGSPRGITSVTTPDGRFTVLMPHAERVFRTVQHSWHPDGWG
                     EDSPWMRMFRNARKWVG"
     gene            15372..16718
                     /locus_tag="VM406_09345"
     CDS             15372..16718
                     /locus_tag="VM406_09345"
                     /inference="COORDINATES: protein motif:HMM:NF005460.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HVL76209.1"
                     /translation="MNPPVTRSTADLSSEARVPLVEDCLAQAANLPAVFTRLYPDAAR
                     AAAAHADALRSAGVQTSPLAGLPVSIKDLVDVAGEITLAGSIALRDAAPAASDAPVLQ
                     RLRAAGAAIVGKTNMTEFAFSGVGINPHYGTPANPSDREVARIPGGSSSGAAVSVAAG
                     LCVAAIGSDTGGSIRIPAALCGIVGFKPTARRVPVAGTVPLSTSLDTICAMTRSVDDC
                     ILLDRIIADQALAIPGLPLAGLRLAVPQTLALDGLDAGVSEAFTAALSRLSAAGAHVV
                     DAPFALLAEAADINRYPGVEAYAWHRELIARREAEYDPRVARRIKLGAAMSAADYIGM
                     ERRRRDWIARMEDALAGFDAMLMPTVPMVAPPIAPLLESDERFFQTNAQLLRNTSTIN
                     LLDGCALSLPCHAPDMLPVGLMVTGPAMADARVLAVARAIERTLQQQQDRESRVAA"
     gene            16818..>17591
                     /locus_tag="VM406_09350"
     CDS             16818..>17591
                     /locus_tag="VM406_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVL76210.1"
                     /translation="MTQKQIAVVGGGVVGVCTAYFLAAAGHEVVVIERRQNVAQESSF
                     GNAGMVAPAYSGPWAAPGMPARVLGGLFKSESAIAFKPRLDRTLWHWLRRWRAECDLE
                     RFRINKARMQRVAFYSRALLEQLRDHYQLEYERTQGVLQLFRSQRDIRMAEPVIAMLS
                     EYGVPHRMLDPDAARAREPGLSAHAELAGALEFPQDEAGNCPLFTRRMKHIAATIGVQ
                     FHVAATVEAIELESRGVGLRIDGQRFPVDAVVVAAGAASA"
BASE COUNT         3016 a         5841 c         5551 g         3183 t
ORIGIN      
        1 gcctgggtcg ggctatcttc ggcaatcagt atttccgcac gctcggcccg gatcacatgc
       61 actccattct gatgctgatc cgacagcgca gcgggcagac ggttcttcgc ggcagcaacg
      121 ccacgttgcc ggatggagaa accggaaccg ccgaacgccc ggttcatcca acagacggaa
      181 aatacggaga ggattgcatg catccaatga tgaagggagc gctggctggc gccgtggcga
      241 cgattccgat gaccatcgtg atgagcggct tgttccgccg gctgccgcgc gagcaacagt
      301 atcctctgcc gccggtgctg atcacttcgc gcgtggcgcg ccacggcgac ggggaaccgg
      361 ccttgcaggg cggccggctg accgccgccg cgctggctgc ccacttcgcc tatggcgcgc
      421 tgaccggcgc catgtacccg ctgctcgagc gcacgcgggc gctcgacggc ccgccggcac
      481 tgaccggtcc cgcctacggc ctggccgtct gggcggcgag ctatctcgga tggatcccgg
      541 ctgcgcgcat ccttacgccg gccacgcgcc atccgacgca gcgcaacgca ctgatgtgga
      601 ccgcccatgc ggtatggggc gcggcgctgg ccgccgtctg cggcctgctg cagcagcgcg
      661 agccggcttc gcaagaactg cagaccgcct ggggcacacg ggccggcaat ctcgatcggc
      721 ctgaataccc cggaaacaaa caaggccgga tgcagccgcg cgaacggctg catccggccc
      781 atcaggcaga cgcgcgaatc agttgaaggt cttgccctct gcggccagcc ttgccagcaa
      841 cggcgccggc ttccaggctt cgccatggcg tcccttcgca aagcgctcca tcgccatcac
      901 cacgttctgc aggccgacgg tgtcggcata gaacatcgga ccgccgcgat gcagcgggaa
      961 gccgtagccg gtcaggtaga ccatgtcgat gtcggaagcg cgctgggcga tgccttcctc
     1021 caggatgtac gcgccttcat tgacgagcgc gaacaccagc cgctgcacga tctcctcgtc
     1081 gctgatcttg cggcgctcga tgccgagatc ggccgagtgc ttgacgatca tctcgttgac
     1141 cggttgcgac ggatacggct tgcggtcgcc cggcttgtag tcataccagc cggccgaggt
     1201 cttctggccg aagcggccca tctcgcacag caggtcggcc gtcttcgaat aggtgacctc
     1261 cggcttctcg acgtagcggc gcttgcggat cgcccagccg atgtcgttgc cggccaggtc
     1321 gcccatgcgg aacggcccca tcgcgaagcc aaacttctcg attgccttgt cgacctgctc
     1381 cggcagacag ccctcttcga gcaggaagcc ggcctggcgg ctgtactgct cgatcatgcg
     1441 gttgccgatg aagccgtcgc acacgccgga caccacgccg gtcttcttga tcttcttcgc
     1501 cagcgccatc gcggtcgcga gcacatcctt gccggtcttg tcgccgcgca cgatctccaa
     1561 cagcttcatc acattggccg ggctgaagaa atgcaggccg atcacatcct gcggacgctt
     1621 ggtgaaggac gcgatcttgt tgacgtccag cgtcgaggtg ttcgaggcca ggatcgcgcc
     1681 cggcttcatc acttcgtcga gcttcctgaa cacctgctcc ttgacgccca tctcctcgaa
     1741 cacggcctcg atcacgatgt cggcctgcgc tatgtcgtcg tacgacagcg tgccggagat
     1801 caggcccgtg cgctgctcga atttctcggg cgacagcttg cccttcttca tcgtgttctc
     1861 gtagttcttg cggatcgtcg ccaggccgcg gtccagcgct tcctgcttca tttccaggat
     1921 cttgaccggg atgccggcgt tggcgaaatt catcgcgatg ccgccgccca tcgtgccggc
     1981 gccgatcacc gcggcggtct tgatctcgcg cttgggcgtg tcttccggca catccgcaat
     2041 cttgctcgcg gcgcgctcgc cgaagaacgc atggcgcagt gccttcgact cggtggtctg
     2101 caccagttcg aggaacagcg aacgttcata ggctatgccc tcgtcgaact tcttcgtgat
     2161 ggccgcttcc accgcatcca cgcatttgag cggcgccggg aacggaccgg ccatcgcgcg
     2221 taccgtgttg cgcgagaact gcaggaagcc ttcgtagttc ggatagtcga tcttctggtc
     2281 gcgcaccttg ggcagcgggc gcgcatcggc cacttcgttc gcgaatgcga tcgcggcttc
     2341 caacaggtcg ccttcggcca tcaggttgaa cagcgcggtc ttcgccagct tctccgacgg
     2401 caccggattg cccgagacga tcatgttcag cgccagctcc aggcccacca cgcgcggcag
     2461 gcgctgcgtg ccgcccgccc ccggaagaat gcccagcttg acttcgggca gcgcgatctg
     2521 cgcgccgggc gctgcgacgc ggtagtggca gccgagcgca agctccagtc cgccgcccat
     2581 gcaaaccgtg tggatcgcgg cgatcaccgg cttggtggcg ttctcgacgg cactgatgac
     2641 ggtgtgcagc gtcggttcgg ccagcgcctt cggggaattg aattcgcgga tgtccgcacc
     2701 gccggagaat gccttgccgg cgccggtgat gacgatcgcc ttgacggcct cgtcgttcat
     2761 ggcctgccgc aagccgtcga ccacagcgga gcgcgtggcg tggcccaatc cgttgaccgg
     2821 aggattgttc agcgtgatca cggcaaccga gccgtgcacc ttgtattctg cagtcatgtc
     2881 tcttcctttt ctggatggcg gtgggttcag gataagcgaa ccaactatac cgcaaaaaag
     2941 cacgctcgta ttattttgcc ttcaaaaagg aagcgcggat ctcggccccg tctgaccagc
     3001 acggtttgct actggaatgc cgttcgctga agcaagggcc ctgtacttgt cgctccagcg
     3061 caggcgggag cccggatcga acacaagccc tcctacgtgc gttggaactg ggttcccgcc
     3121 tgtgcggggg cgcctagggg aacgacatag caagggcaat cgatccagcg atcggtttca
     3181 taggtctaag cggcacccct gggctgccca ctacgcgcga gcaggcagca tccgcgccga
     3241 cccactcaat tcgccttcat ccgcagcagg gcgccacgtc gctggtcgtc cgcataggca
     3301 taatcgaccc gccccttcgc caccacgccc atcagcagca tgttctgcgt gatcgcgtca
     3361 tggtcctcgc gcgagaacgg acggtgatag ctcgtgatca caccctggta acgcatgcgc
     3421 aggtcctcca gcgcttcctt gatccgcgga ccctcggtcg agttggcctg ccgtagcgcg
     3481 aggtaaagca ggtgcatgcc gtcatagcct tgtgcggcac tcatcggcga cgggatgaga
     3541 tcggtgttgt acgcgcgccg atacgccagc aggaagctgt tctttgcgct tgagccgacg
     3601 tcctggatga aggtctgcgg catcagcgcc ccctcgccgg cccgtcctgc cggctcgatg
     3661 aaattgagca tcgagaaggt ccagcttccg agcatcggca cgttccagcc gattgcctcg
     3721 cggttgcggg cgattgcggc catttccggg ccgatgcccc agaccaccag tgccctggca
     3781 ttgctggcgc gcgcgcgtcg cacctgctcg ctcatgtcct gatcgccgat cctgaagcgc
     3841 tcctgtgcgg cgacctccac gccagccgcc ttcgcctcgc gcagaaacgc ctgcagcccg
     3901 gcttcgccat aggccgtcgc atcggccagg atcgcggcgc tccccaggcc cttgcgcttg
     3961 aggtcggccg ccaccacgcg cgcctccaga tccagcgtcg gcgaaacgcg gaagatgaag
     4021 ttctcgggcg catgcggcgg cgcgtacttg cgcgtgagcg tggtgccggt ggataccgcg
     4081 atcatcagcg gaatgcgcgc cttctggtac gcgtcgatcg aggccagccc gacgccggtg
     4141 ttgacgatgc cgatcgtcgc caccacccgc atctgcgtga gctcatcggc gatctttgcg
     4201 ccgagatcgt tgttggcctg atcgtcgcgc tcgaccagtt ctatccggcg gccgttcacg
     4261 ccgccgatac cattgatctc gtccaccgca aggcggatcc cgttgagcat gctcagtccc
     4321 atcggcgctg aaccgccggt aaacggtccg gatacgccga tgcgtatcgg ctcggcatgc
     4381 tgggcaaggg caaggaaagg cagcgcggcg cacaggacga aagcgctgcg caggttggcg
     4441 atccgcatat aaagtctcct ccgtaggttg tgaggctggt cgccataggg cgccgcccct
     4501 ttgttccaac gcaggaggca ttcgcaggag cggatgcgac tgcggtggca gcatcagtct
     4561 accgaccgca accgaatccg tgaggaaaat tttgaatggg agcgttgaat cccatgcatg
     4621 taccagtcgc gtctcccgct acgcgttccg cggcgccgcg cggagcggtc gggttcacgc
     4681 cgtgggcagg cggtgatcct tgaactgttc gcggatgcgg ttcttcagca ccttgccggt
     4741 cgcgccgatc ggcaaggcgt cgacgaacac gacatcatcc ggcatccacc atttggcgat
     4801 cttgccctcg tagaaggcca gcagctcttc cttcttgagt tccgcgccgg gcttcttcac
     4861 caccaccagc agcgggcgct cgtcccactt cggatggcgc acgccgacgc aggcggcctg
     4921 ctgcacggcc ggatgggcaa ccgcgatgtt ctctaggtcg atgctgccta tccattcgcc
     4981 gccggacttg atcacgtcct tgctgcggtc ggtgatctgc atgtagccgt ccgggctgat
     5041 cgtggcaacg tctccggtcg ggaaccagcc gtcctgcagc acgtccccgc cctcgccctt
     5101 gaaataggaa ccgatgaccc acggtccctt gaccagcagg ttgccatagg tcttgccgtc
     5161 ccagggcagt tctgcgccga cgtcgtcgac gatcttcatg tccacgccgt agatcacatg
     5221 cccctgtttc tcgagaatcg cctgctgctg ctccggcgcc aggctgacat gatgggactg
     5281 cagcgtgcag gtggtgccga gcggggacat ttccgtcatg ccccaggcat gaatgacctc
     5341 gacgccgtac tgctcgcgga aggccttcat catcgccggc ggacaggcgg agccgccgat
     5401 gatggttcgg cggaacgacg agaagcgcag cttgttctcg ccgacatggt tgagcaggcc
     5461 gagccagacc gtcggcacgc cggccgagaa cgtcaccttt tcctgctcga agagttcgta
     5521 gatcgacttg ccgtccagcg cgggccccgg aaaaaccagc ttcgcaccgt tgagcggcgc
     5581 cgaatatgga aggccccagg cgttcacatg gaacatcggc acgaccggca acaccacgtc
     5641 gcgcgcggag atgttcatcg catccggcat cgacgccccg tatgaatgca ggatggttga
     5701 ccggtgcgaa tacaaggccc ccttcggatt gccggtggtg cccgaggtgt agcacaggct
     5761 ggaggccgag ttttcgtcga acaacggcca ttcgtactga tccgagtggc cgtccaccag
     5821 gtcctcatag cacatcaggc cggaaagctt ggactcggcc ggcatgcgct cgcggtcgca
     5881 catcagcacg tagccgcgca ccgtcttgca atgggatgcg aacgcctcga ccagcggcag
     5941 gaaagtcagg tcgaagaaca gcaactggtc ttccgcatga ttggcgatat acgcgatctg
     6001 ctcgggatgc aggcgcggat tgatcgtgtg catgaccgcg cccgagccgg agatcgcgta
     6061 atacagctcc atgtgacggt aaccgttcca ggccagggtg gcgacccggt cgcccatctt
     6121 cacgccgtgt gcggcgagcg cattggccat ctggcgggca cggcgatggc agtcgcgata
     6181 agtgtagcga tgaatatcgc cctccacgcg ccgggagacg atctcgttgg ttccgaaatg
     6241 gcggtcggca tgccggatga tgctcgcgat gagcaggggc tggctcatca tctggcccat
     6301 cagggggctc ttagggtatt ccgtcatcgt gtctccaagg atgagtgcgg gaggtggaag
     6361 gcaaattttt gaacgaccgt gctactcagt caacggattt tcattccgcg atgcagcaca
     6421 aagcgcggcg cgccatagtg ataccaccgc cggccgggtg ctttgttctc tgtcaaaata
     6481 ccgccttgcc ccgctcgccg gacggctttc ccctccggcc cgaggcggga accaagcctt
     6541 gcagcgcgca ttccattaag caggcgcgta tcccgcgttc ttgcctgaaa gatgttcatg
     6601 aatgaaatcc ggccggtcgc ggccccttac cggctcgaca gtctcccctt gcagaacgga
     6661 ttcgccgcct tgccgccggc gttctatacg cgcctgcagc cgacgccgat tcccgctccg
     6721 tatctggttg gcgcaagcgc tgccgcggcg cggctgatcg gactcgatgc atccgagttc
     6781 gcgcacgagg aattcctgga aaccttcgcc ggcaaccgtc tgttgcccgg ttcgcagccg
     6841 ctcgcggccg tctattccgg tcaccagttc ggcgtctggg ctggccagct aggcgacggc
     6901 cgcgcgatcc tgctcggtga tgcggcacac gcacagggcg ctgggaggct ggaactgcag
     6961 ctgaagggat cgggcctgac gccctactcc cgcatggggg acggtcgcgc ggtgctgcgc
     7021 tcatcgatcc gtgaatttct gtgttcggag gcgatggccg ggctcggcat cccgacctcg
     7081 cgcgcacttt gcgtggtcgg ctccgacatg ccggtcatcc gtgaaacggt cgagacggcc
     7141 gcagtggcga cgcgaatggc gccgtcgttc gtgcgcttcg gctccttcga gcactggttc
     7201 tataacgagc gccagcacga actgaagctg ctggccgact acgtgatcga caatttctac
     7261 cccgcgctgc gcgatgcaga caacccgtat cgcgcgttgc ttggcgaagt cacgcgacgc
     7321 accgccgaac tgatcgccca atggcaggcg gttggcttca tgcacggcgt catgaacacc
     7381 gacaacatgt ccatcctcgg catcacgatc gattacggac cgttcggctt catggaagcc
     7441 tttgacgtgc gccacatctg caaccatagc gaccgtcagg gccgctatgc ctatcagatg
     7501 cagccgcgga tcggccaatg gaactgctat gcgctcggcc aggcgctgct gccgctggtg
     7561 ggcagcgtcg aggaggcgca ggacacgctc gagatctaca agccggccta tgacgacaag
     7621 ctcgacgcgc tgctgcatgc gaagctcggc ctgcgcgaga agatggaaga ggacggcgaa
     7681 ctgatcgatg caatgttcgc gctgatgcag gccaatcatg tcgatttcac gctgttcttc
     7741 cgccgcctcg ccgaccttgg ccgtgacccc gggcctggcg acgaaggcgt gcgcgacctt
     7801 gtcatcgacc gcgccgcgtt cgacgcctgg gcggtgaagt accgccagcg ccttgctcgc
     7861 gaaaacagca acgacgccga gcgccgcgcg gcgatgaacc gggtcaaccc gaaatacgtg
     7921 ctgcgcaatt atcttgcgca gaccgccatc gagaaggcgc aggacaagga tatttccgaa
     7981 gtcgcgcgcc tgctcgccat ccttgaacgt ccgttcgacg agcagcccga gcatgaacac
     8041 tacgcggcgc tgccgccgga ctgggcgagc catctggaag tgagttgttc atcatgagtg
     8101 agaaagtcgt caagagcgac gccgaatggc gcgcgcagct ggaaccaatg gaatacgagg
     8161 tcacgcgcca cgccgccacc gagcgcgcgt tcaccggccg ctattgggat catcacgaag
     8221 ccggcatcta ccgctgcgtc tgctgcggca ccgcactgtt cgcctccgac accaagttcg
     8281 attccggctg cggctggccg agttacttcg aacccatcga tcctgccaac gtgatcgagc
     8341 gtatcgatcg cagtcatggc atgatacgca ccgaaattct gtgcgcgatc tgcgatgccc
     8401 atctcgggca cgtgtttccg gacggacctc cgcctactgg actgcgctat tgcatcaatt
     8461 ccgccgcgct gcagttcgaa ccggcgcgga ccgccagttc tgcctgacaa ggaaaaaccc
     8521 aaacgatgaa gttcctgttc gatctgttcc ccgtgatcct gttcttcgcc atgttcaagt
     8581 ggggcgaaag caatccggaa gccgctcttg ccttgtcgga ccgctacctg tcagcgctga
     8641 tagccggagg cggagcaacg gtcgaacagg cacccatcct gctcgccact gcggttgcca
     8701 tcctcgccac agccgcccag attctctacc tggttgcgcg cggaaagaag gtcgagtcga
     8761 tgttgtggat ctcgctggcc atcattgcgg tgttcggcgg cgcgacgatc tacttccaca
     8821 acgagacctt catcatgtgg aagcccaccg tgctctactg gctgttcgcc ggcgtgctgc
     8881 tcggcgccca ggccctgttc ggccgcaacc tgatccgcca gatgctggag aagcaggtcg
     8941 cgctgccggg tccggtctgg caccggctga acctgtcgtg ggcggcgttt ttcgtcgcga
     9001 tgggtctggt gaatctatat gtcgcctaca gcttctcgca aagcacctgg gtcaatttca
     9061 agatgttcgg attcaccggt ctcatgctcg ctttcgtgat tgcacagagc atgatgctgt
     9121 cgaagtacat caaggaagag tgagcttgcg atgagcgcgc gcatcgagaa aatgcgcagc
     9181 ctgctggctg caactttcac acccctcgac tgtcaactcg tcgacgaatc gcacttgcac
     9241 gcaggccacg ccggcgcggc aggcggggcc ggccattaca ggctgcacat cgtttccgag
     9301 cgtttcgaag ggcttaaccg tttggccaga caccgactgg tgtatgattg cctgcgcgac
     9361 atgatgccgg gcgaaatcca cgccctgaac atcgccgcag tcgcgccctc ggagttgaac
     9421 cgctgaccag cggcatcaca tcatcggcac cgcattgcgg ccccgtccat ccgtttttta
     9481 cccacacatc gagagttagg aagacacatg accttcgtac aacccgcccg cctgctgatg
     9541 gcgctggtcg ccgcgatcgc cctgcccgcc atggcgcaga atctggccgt cgtgaacggc
     9601 aagccgattc cgtcctcgcg cgccgacacc atgatcaagc aactgcaggc ccagggccag
     9661 ccggattccc ccgagctgcg cgcgatggta cgtgaagaac tgatcaaccg cgagatcctg
     9721 atgcaggaag cgaaccgccg cggcgtgacc aagaatccgg acgtgcagaa ccaggccgag
     9781 atcgcgcgcc agtcgatcgc gatccgcgcg ctggtgcagg actatctcga gaagaacccg
     9841 gtgtccgatg ccgagatcaa ggccgagtac gacaagttca aggcccagtc cggcgacaag
     9901 gaataccatg cccgccacat cctcgtcgaa accgaggaag aagcaaaggg catcatcaac
     9961 aagctcaagg gcggcgcgaa gttcgaagag ctggccaagc agtcgaagga tcccggttcg
    10021 gccgcggccg gcggcgatct cgactgggct ccgccgggcg ccttcgtcaa gccgttctcg
    10081 gatgcgatgg tctcgctgca gaagggccag ttcacacagg ctcccgtgca gacacagtac
    10141 ggctatcacg tgatcaagct agaagacgtg cgcgacgcac agctgccctc gctggaagaa
    10201 gtcaagccgc acatcgcgga atcgctgcag cagcagaagc tgcagggctt ccaggaagag
    10261 ctgcgcaaga aggccaagat ccagtaagcg cgcaattccc gcacaagggc gcttcatccg
    10321 aagcgccttt tttctttcag agatgagccc ccacccatcc atcgaacacg cagccgacct
    10381 ggagaccgtc gtcgacgccc gccacccggt gcgcccggtc atctggcgcg gccgatccgg
    10441 cttcgccaaa cgcccggcca gccttcgctc gcacgaaagg atcctgctcg cccttgcccc
    10501 gctgatccgg cgctggctcg gcaaggacct gccgtttcat ccggttcgct atgagaaggg
    10561 atggccggtc gaagtgcagc gcctacgccg gctgcgcgct gccggctgtg cggtcccgga
    10621 agtcctgcac gcggagcgcg acctattcgt cagcagcgac gccggcccga cgctcgcgca
    10681 actgctggcg gaccaggtcg acgccggcgt ccgacttcaa tggctgaacg aagccgcacg
    10741 cgatctggcg gcattccacc aggccggaca ctggcacggt ggcgcgcagg tacgcaatct
    10801 cgtgcgtacg ccggaaggaa cgctcggccg gatcgatttc gaaaccgcgc tggatgcgcg
    10861 ctttccgctg gcgctgctgc aggcattcga cgccgcgctg ttcttcagca gcatcgtgcg
    10921 cagccgcgaa tctgcagccc tgcccgatat cgcgagggcg tatctggacg acgcgccgga
    10981 agacgcccgc cgtgtactgc gccgcggcct gcccctgctt cgcagaatgg cgcgatcgaa
    11041 actgctcaga aagctcgcgc cgaaggaagc ggaacgcctg cgcagcatcg ctgcgttgcc
    11101 gctggacggc tgagcatcct agaccgggac gcgatcccgg ctttcatccg gaccgccgaa
    11161 cccggctggg atcttcatcc cagccagccc tgcttcaaca agctgagaat caaccaaccc
    11221 acttgcgcgc attgcggaac atgcgcatcc acggcgaatc ctcgccccag ccgtccgggt
    11281 gccaggaatg ctggaccgtg cggaacacgc gctccgcgtg cggcatcagc accgtgaagc
    11341 ggccgtccgg cgtggtgacg gatgtaatgc cgcgcggcga gccgttcggg ttgaacggat
    11401 aggtttccgt cacctgtgcg cgattgtcga tccagcgcat cgcgacgatc gcttcgtcga
    11461 tgttgccggt ccgcgagaag tcggcataac cttcgccatg cgcaacggcg atcgggcttt
    11521 gcgtgccggc catcccggcg aagaagatcg acggggactc ggccacttcc accatcgcga
    11581 aacgcgcctc gaactgttcg gacttgttgc gggtgaactt cggccaggcc tcggcgcccg
    11641 ggatgatgga cttcaggttg ctcaacatct ggcaaccgtt gcaaatgcca agcgcaaagg
    11701 tgtcggcgcg cttgaagaaa ccggagaact ggtcggacag cgcggcattg aacaggatgg
    11761 tcttggccca accttcgccg gcgcccagca cgtcgccata ggaaaagccg ccgaccgcga
    11821 tgaagccctt gaagtcgtcc agcttcgccc gcccggcgat caggtcgctc atgtgcacgt
    11881 cgactgcctc gaagccggac ttgtgcatca cccaggcggt ctcgacatgg gaattgacgc
    11941 cctgctcgcg caggatggcc acgcgcgggc gcgcgccggt ggcgatgaac ggcgcggcga
    12001 tgtcttcctg cggatcaaac gcgaggcgcg gcgacaggcc gggatccttc tcatcgagga
    12061 gacggtcata ttcggcatct gcgcaggtcg gattatcacg caggcgcgcg atgcgccagc
    12121 tggtttcgct ccacgcgcgc tgcaggtcga cgcgcgtctg agaaaacacc atcttcgcgt
    12181 cgcgcatgag ttcgataacg ccacgcgcgt tcggcttgcc gatgatgtgg ctgcaggcgc
    12241 cgaggttatg gctgcgcagc acgttcatca cctcggattt ttcatccgcg cgcacctgga
    12301 tcaccgcgcc cagttcctcg ttgaacaagg cgcgcagcga catctcgttg cggcgctcgc
    12361 cgacctgggt ggcccagttc ttcgcgtccc ccgagtcgga agcgtgctcg ccttccatcg
    12421 cgagtatgtc gacgctgacc gacacgccgc aatggccggc aaaggccatc tcgcacaacg
    12481 tggcgaacag gccgccgtcc gagcggtcgt ggtaggcgag cagcctccct tccgcattga
    12541 gctgctggat cgcggcgaag aagttcttca ggtcttgcgg atcgtcgaca tccggcactt
    12601 cgttaccgat ctgctgcatc acctgcgcga gcgcggacgc acccatgcgg ttcttgccgc
    12661 ggccaaggtc gatcaggatc agcgcggtgt cgccaagatc ggtgcgcaac tgcggcgtga
    12721 gcgcgcggcg cacgtccggc accggcgcga acgcggagac gatcagcgac accggcgaga
    12781 tcacttcctt gctgccctgc gcatcgttcc atgtcgtgcg catcgacagc gaatccttgc
    12841 cgaccgggat gctgatgccc agagcggggc acagttccat gccgactgcc ttcacggtat
    12901 cgaacagcgc cgcatcctgg cctggctggc cgcaggcagc catccagttg gcggaaagct
    12961 tgatgtcgga aatgtccgcg atcggcgccg cggcgatgtt ggtgatcgcc tcgccgaccg
    13021 cgatgcggcc ggaagcagcc gcgtcgatcg cggccagcgg cgtgcgctcg cccatggcca
    13081 ttgcttcgcc gcgatagcct tcgaaactca tcgcggtgac cgcgcagtcg gccaccggca
    13141 cctgccacgg gccgaccatc tggtcgcgca cggtggtcgc gccgacggta cggtcgccaa
    13201 tggtgatcag gaaggatttg tcggccaccg tgggcagccg cagcacgcgc tgggcggctt
    13261 cgagcagatc gacgccggtc aggtcgacgg gcgcatagtc ggtatgcacg tggcttacgt
    13321 cgcgcagcat cttcggcggt ttgccgagca gcacgtccat cggcatgtcg accggatagt
    13381 tgtcgtgctc ggggtcgatc accttcagct gccgttcctc ggtcgccacg ccgaccaccg
    13441 cgaacgggca gcgctcgcgt tcgcacatcg cgcggaacag ctccaggctc gccggcgcga
    13501 tcgccagcac gtagcgctcc tgcgattcgt tgctccagat ttccttcggc gcgaggccgg
    13561 attcctccag cggcaccttg cgcagatcga agaccgcacc gcgcttggca tcgttggtga
    13621 tttcagggaa ggcgttggag aggccgccgg cacccacgtc gtgaatcgag agaaccggat
    13681 tgtcgtcgcc gagcgcccag caggcgttga tcacttcctg cgcacgccgc tccatttccg
    13741 gattgccgcg ctggacggaa tcgaaatcga ggtcggcagt gttggtgccg gtcgccatcg
    13801 acgatgccgc cccgccgccc atgccgatgc gcatgccggg gccgcccagc tgaatcagca
    13861 ggctgccgac cggcaggtcg tgcttcttcg tatgcttggc cgagatattg ccgatgccgc
    13921 cggcgatcat gatcggcttg tgatagccgt agaccttgcc tccgacattc tgctcgtagc
    13981 tgcggaaata gccgccgaga tttgggcggc cgaactcgtt gttgaacgcc gcgccgccga
    14041 tcgggccttc gatcatgatc tgcagcggcg aagcgatgcg ttccggcttg ccgtagatgc
    14101 cggcctgcgc atgcggggcg cgttgcgcca ccggctcggc cacgtcgcgc gcgttctccc
    14161 acggctgcac cgcgtgtgtc agcatcaggt tcgaaaccgt gaagccggtc agccccgcct
    14221 tcggcttggc cccgcggccg gtcgcacctt cgtcgcggat ctcaccgccg gccccggtcg
    14281 aggcgcccgg gaacggtgcg atcgcggtcg ggtggttgtg cgtctcgacc ttcatcagga
    14341 tgtgggtcag ctcctccgag gcgccgtaga cccggccctc gccgcgcgga taaaagcgcg
    14401 agacggtcgc gccctcgatg accgacgcat tgtcgctgta agcgacaatc gtgcccttcg
    14461 ggttctgctg gtgggtgttc ttgatcatcg cgaacagcga cttgtcctgc ttctcgccgt
    14521 cgatgatcca gtccgcgttg aaaatcttgt ggcggcagtg ttcgctgttg gcctgcgcga
    14581 acatcatcag ctcgacgtcg gtcgggttgc gccgggcctg cgtgaatgcg tcgaacaggt
    14641 agtcgatttc atcttcggac agcgcaagcc cgagttcgct gttggcgcgc accagcgcgt
    14701 cgcggccgcc cttctgcaca tcgacgaatt cgagcggccg ggcttccagg gtgtggaaca
    14761 accccgccgc ctcgttcgca tcgcgcagca ccgtttccac catgcggtca tgcaacaggc
    14821 ccgtaaccgc tgcgacggct tccgccgaca gcttcttcgc gccgcccagc aggccggatt
    14881 tcatctgcac cgcatacagg acgccgcgtt cgacgcgatg aatgtgcgtc atcccgcagt
    14941 tgtgcacgat gtcggtcgcc ttgctcgccc acggggaaat cgtgccgaag cgcggaatca
    15001 cgacgaactg ctcgccttcg ctgttgccag caaacggttc cccgtagttc aggaggccgg
    15061 ccaggcgctc gaggtccatg gcgctcggtt cgcgcggggc atcgacgaaa tgcagatagc
    15121 gaccggtgat gccgacgatc gccggttcga cagcttgcag ttgggacagg aggcgttggg
    15181 tacggaaggc agacagggcg ttggagcccg gcaggatcag catggcgcgc gatggaggca
    15241 ggagaggcag caaccgtccg gattcagacg gcattctgcg ggttgaaacg gaaaccgcga
    15301 ttataccgtg ccgcatcatc gccactgctg ctgtaccatc attgcccggc cacatttctt
    15361 tcactgtcgt catgaatcct cccgtcaccc gctccaccgc cgacctgtcg tcggaagcgc
    15421 gcgtaccgct ggtcgaagac tgcctcgccc aggcggcaaa cctgcccgcg gtctttaccc
    15481 gtctctaccc ggatgcggcg cgtgcggcgg ccgcgcacgc ggatgcgctg cgctcggccg
    15541 gcgtgcagac atccccgctg gccggcctgc cggtatcgat caaggacctg gtggacgtgg
    15601 ccggcgaaat cacactggcc ggttcgatcg cgctgcgcga tgccgcgccg gcggcaagcg
    15661 acgcacccgt tctccagcgc ctgcgcgccg ccggagcggc gatcgtcggc aagaccaaca
    15721 tgacggaatt cgcgttctcc ggcgtgggca tcaacccgca ttacggcacg ccggccaatc
    15781 cgtcggaccg cgaagtggca cgcattcccg gcggttcttc gtcaggcgca gcggtgtcgg
    15841 tggccgcggg cctgtgcgtg gcggcgatcg gttcggacac gggcggctcg atccgcatcc
    15901 cggcggcgct gtgcggtatc gtcggcttca agccgaccgc acggagggtg ccggtcgcag
    15961 gaacagtccc gctctcgacc agcctggaca cgatctgcgc gatgacacgc tcggtcgacg
    16021 actgcatcct gctcgaccgc atcatcgccg accaggcgtt ggcgatcccc ggcttgccgc
    16081 ttgccggact gcggctcgcg gtgccacaga cactcgcgct ggacggcctg gacgccggcg
    16141 tgtccgaggc attcacggcc gccctctcgc gcctgtcggc cgccggcgcg cacgtcgtcg
    16201 acgcgccgtt cgcgctgctg gccgaggcgg cggacatcaa ccgctatccg ggggtcgaag
    16261 cctatgcctg gcaccgcgag ctgatcgcgc ggcgggaagc cgaatacgat ccgcgcgtgg
    16321 cccgccgcat caagctcggt gcggcgatga gcgcggcgga ctatatcggc atggagcgcc
    16381 gccgccgcga ctggatcgcg cgcatggaag acgcgctcgc cggcttcgac gcgatgctca
    16441 tgccgacggt gccgatggtg gcgccgccga tcgcgccgct gctggaatcg gatgaacgct
    16501 tcttccagac caatgcgcag ttgctgagaa atacatcgac gatcaacctg ctcgacggtt
    16561 gcgcgctctc gctgccctgc catgcgcccg acatgctgcc ggtcgggctg atggtcacag
    16621 gtccggcgat ggccgacgcg cgcgtgctcg cggtcgcgcg cgcaatcgaa aggacgctgc
    16681 aacaacagca agaccgggag tcgcgcgtcg ccgcctgagt ttccgcgggg ttttccctgc
    16741 atttccgcgt accggagcca ggatgctgcg tctaaaatgt gggccttttc ctgagtgcgc
    16801 gagaacggat tgacgcagtg acacagaaac agattgcagt cgtcggcggt ggcgtggtcg
    16861 gcgtctgtac cgcgtatttc ctcgccgccg ccggacatga agtcgtcgtg atcgaacgcc
    16921 ggcagaacgt cgcccaggaa tccagcttcg gcaatgccgg catggtcgcg cccgcctatt
    16981 ccgggccctg ggccgccccg ggcatgcccg cccgggtgct cggcgggctg ttcaagtccg
    17041 aatcggcgat cgcgttcaag ccgcgcctgg accgcacgct ctggcactgg ctgcgccgct
    17101 ggcgcgccga gtgcgatctc gaacgtttcc gtatcaacaa ggcgcgcatg cagcgcgtcg
    17161 cgttctacag ccgcgcgctg ctcgaacagt tgcgcgatca ctatcagctc gagtacgaac
    17221 gcacccaggg tgtgctgcaa ctgttccgca gccagcgcga catccgcatg gccgaaccgg
    17281 tcatcgcgat gctgtccgag tatggcgtgc cgcacaggat gctcgacccg gacgctgcgc
    17341 gtgcgcgcga gccgggcctg tcggcgcacg cggaactggc cggcgcgctc gaatttccgc
    17401 aggatgaggc gggaaactgt ccgctgttca cgcgccgcat gaaacacatt gccgccacaa
    17461 ttggcgtgca gttccacgtg gccgcgacgg tggaggcgat cgaactcgag agtcgcggtg
    17521 tgggcctgcg catcgacggc cagcgctttc cggtcgacgc ggttgtggtc gccgccggcg
    17581 cggccagcgc c
//
LOCUS       DATLGF010000305         2706 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_119189, whole genome shotgun sequence.
ACCESSION   DATLGF010000305 DATLGF010000000
VERSION     DATLGF010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119189"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..760
                     /locus_tag="VM406_09355"
     CDS             104..760
                     /locus_tag="VM406_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HVL76211.1"
                     /translation="MNQAIQTLTVYALPLILAITLHEAAHAYAAKRLGDATAYMMGRM
                     TLNPVKHIDPIGTIAMPLILFAVGSPFLFGYAKPVPVQFGNLRNPRRDMALVALAGPG
                     ANMVMAFLWLVFAILLRVVGIDEPFFLRVAEAGVLLNLVILAFNLFPVPPLDGGRVMT
                     SLLPNRLAYRFAQLEPYGFFIVIGLVLLGALRYWMIPVMEAAHAVLMILVSPLTFLLR
                     "
     gene            770..1972
                     /locus_tag="VM406_09360"
     CDS             770..1972
                     /locus_tag="VM406_09360"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HVL76212.1"
                     /translation="MYPERVVSGMRPTGAMHLGHYHGALKNWIKLQSELPCMFFVADW
                     HALTTHYDDPSIIETSTWDMLIDWLAAGIDPAQATLFIQSRVPEHAELHLLLSMSTPL
                     GWLERVPTYKDQQEKLADRDLATYGFLGYPLLQAADVLIYRATLVPVGEDQIPHIEMM
                     REIARRFNHLYGKEKGFEEKAQEAVKKLGSKRARIYMELRNEYQEKGNDDALAQAQAM
                     LDEAQNLSMIDRERLFGYLEGSRKLILVEPQVKLTEASRLPGLDGQKMSKSYGNTITL
                     REEKDSVIKKVRTMPTDPARVRRTDPGEPEKCPVWQLHKVYSDEETKQWVQKGCRSAG
                     IGCLECKQPVIDGILKEQEPMRERAQQYLDDPSLVRAIVADGCDRARKLAQETMRDVR
                     EAMGLSYT"
     gene            1977..2519
                     /locus_tag="VM406_09365"
     CDS             1977..2519
                     /locus_tag="VM406_09365"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL76213.1"
                     /translation="MDQPSPWVVRFAPLIPPGEVLDLACGSGRHARLLAALGHPVLAV
                     DRDEAALASARGAGIETLCFDLERGEPWPFAPRRFAGIVVTNYLHRPLFPGLLESIAP
                     GGMLIYETFAAGNERFGRPARAEFLLGRGELLDLLRAADWQVAAFEDGQVSHPRPAVI
                     QRICALRRPAAASEWAELET"
     gene            2596..>2706
                     /locus_tag="VM406_09370"
     CDS             2596..>2706
                     /locus_tag="VM406_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HVL76214.1"
                     /translation="MIQGSIVAIVTPMHEDGSLDLPGLRKLIDWHIAEGTD"
BASE COUNT          470 a          878 c          874 g          484 t
ORIGIN      
        1 cgggcattct agcagagggg gaagttgcgc cgtggcgctt acaggccgaa cagggacggg
       61 tcgccgcgtc cggcgcaact tccccctctg ctagaatgcc cgcatgaatc aagccatcca
      121 gacccttacg gtctatgcgc tgcccctgat cctggcgatc accctgcacg aagcggcgca
      181 tgcgtatgcg gcgaagcggc tcggcgatgc taccgcctac atgatggggc gcatgaccct
      241 caatcccgtc aagcacatcg atccgatcgg cacgattgcg atgccgttga tcctgttcgc
      301 ggtcggcagc ccgttcctgt tcggttatgc caagccggtt ccggtgcaat tcgggaacct
      361 gcgcaacccg cgtcgcgaca tggcgctggt ggcgctcgcc ggtccgggcg ccaacatggt
      421 gatggctttc ctgtggctgg tgttcgcgat cctgctgcgt gtggtcggca tcgatgagcc
      481 gttcttcctg cgcgtggccg aggccggcgt cctgctgaac ctggtgatcc tcgcgttcaa
      541 cctgtttccg gttccgccgc tcgatggcgg ccgggtcatg accagcctgc tgccgaaccg
      601 cctggcctac cgctttgccc agctggaacc gtacggcttc ttcatcgtga ttggcctggt
      661 cctgctcggt gcgctacgct actggatgat cccggtcatg gaggccgccc acgcggtcct
      721 gatgatcctg gtttcgccgc tgactttcct acttcgctaa gaaaagatta tgtatcccga
      781 acgtgtcgtt tccggcatgc gtcccaccgg cgcaatgcat ctcggccatt accacggtgc
      841 gctgaagaac tggatcaagc tgcagtccga actgccgtgc atgttcttcg tcgccgactg
      901 gcatgcattg accacgcatt acgacgatcc gagcatcatc gagaccagca cctgggacat
      961 gctgatcgac tggctggccg ccgggatcga tcccgcacag gcgacgctgt tcattcagtc
     1021 gcgcgtgcct gagcatgcgg agctgcacct gctgctgtcg atgtcgacac cgctcggctg
     1081 gctggagcgg gtgccgacct acaaggacca gcaggaaaaa ctggccgatc gggatctggc
     1141 cacctatggc ttcctcggct atccgctgct gcaggcggcc gacgtgctga tctaccgcgc
     1201 cacgctggtg ccggttggcg aggaccagat tccgcacatc gagatgatgc gcgagatcgc
     1261 gcgccgcttt aatcatttgt acggcaagga gaagggcttc gaggaaaagg cgcaggaggc
     1321 ggtcaagaag cttggcagca agcgcgcccg catctacatg gaactgcgta acgaatacca
     1381 ggagaagggc aacgatgatg cgctggccca ggcccaggcg atgctcgacg aggcgcagaa
     1441 cctgtcgatg atcgatcgcg agcgcctgtt cggctatctc gagggcagcc gcaagctgat
     1501 cctggtcgag ccgcaggtca agctgaccga ggcttcgcgc ctgccggggc tggacgggca
     1561 gaagatgtcg aagagctacg gcaacacgat cacgctgcgc gaagaaaagg acagcgtgat
     1621 caaaaaggtg cgcacgatgc cgaccgaccc cgcgcgcgtg cgccgtaccg atcccggtga
     1681 accggaaaaa tgcccggtct ggcagctgca caaggtgtac tcagacgagg aaaccaagca
     1741 gtgggtgcag aagggctgcc gctcggccgg cattggctgc ctcgagtgca agcagccggt
     1801 gatcgacggt atcctgaagg aacaggagcc gatgcgcgag cgcgcacagc agtacctcga
     1861 cgacccttcg ctggtccgcg ccatcgttgc cgacggctgc gaccgtgccc gcaagctcgc
     1921 gcaggagacc atgcgcgatg tgcgcgaggc gatggggctg agctacacct gaagcagtgg
     1981 atcagccgtc accctgggtg gtgcgctttg cgccgttgat tccgcccggc gaagtgctcg
     2041 acctcgcgtg cggcagcggc cggcacgcgc gcctgctcgc ggcgctcggc catccggtgc
     2101 tcgcggtcga ccgcgacgaa gcggcgctgg cctccgcgcg cggcgccgga atcgaaacac
     2161 tgtgcttcga tctcgagcgg ggcgagccct ggccgttcgc gccgcggcgc ttcgccggca
     2221 tcgtcgtgac caactacctg catcgtcctc tgtttcccgg cctgctggag tcgatcgcgc
     2281 ccggcggcat gctgatctac gaaaccttcg ccgccggtaa cgagcgcttc ggcagaccgg
     2341 cgcgcgccga attcctgctg gggcggggcg aactgctcga tctgctgcgg gcggcggatt
     2401 ggcaagtggc ggcgttcgag gacgggcagg tcagccatcc ccggcctgcg gtcatccagc
     2461 gcatctgtgc gctgcgccgc ccggcggccg catcggagtg ggccgagctc gagacttgac
     2521 gctccctggc ggttgcccca cgggagcaac gatccgctac aatcgaccct ttacattcct
     2581 ctccctacca aaaccatgat tcagggcagc atcgtcgcga tcgtgacccc catgcatgag
     2641 gacggcagcc tcgacctgcc gggcctgcgc aagctgatcg actggcatat cgcggaaggc
     2701 accgac
//
LOCUS       DATLGF010000306         3611 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_150828, whole genome shotgun sequence.
ACCESSION   DATLGF010000306 DATLGF010000000
VERSION     DATLGF010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150828"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VM406_09375"
     CDS             complement(<1..309)
                     /locus_tag="VM406_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL76215.1"
                     /translation="MAAILVIEDNALNRKLVRLILEKAGHQVWQACDAEQGLACAFAQ
                     QPDLILMDMQLPGISGLDATRRLKADPRTQRIPVIAVTAMAMMGDEARCRAAGCDDYI
                     T"
     gene            complement(309..3515)
                     /locus_tag="VM406_09380"
     CDS             complement(309..3515)
                     /locus_tag="VM406_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL76216.1"
                     /translation="MQRLQSVETTLLAGFALILLVFGLLCSVVWHSAKLSAAASASVV
                     HTHEVLNTLSEAAAAMGRASAASHSYVITGSAEQLDLRDESLRDAATAIARIHTLVAD
                     NPSQATRLARVEELVAERRKVFLDYQRIRDSQGLMPAVQVFGSGHPLAVRLQDAIEGM
                     MDEERRLLHERKLEEEATQRSTAISLALLAAALTAVLVFLFSRITRQTRLRQAAAQAL
                     EQSEARLKQILDVLPVAVFISDADGRLSINRAGYDMFPGAAVPDQGTDPYAQFQGTRV
                     SDGRRMQGDDWPLVRAFRKGETVRGEEIEIDSADGTRASLLVTAVPLRKAGTRITGGV
                     AVCQDVTELKRTERGLRIAARFDETRTQALALFNAGFDRDRMFNGLLELLCTKHPFPV
                     AALYHFDDWAGRFRLAAGWGLPAEATREFGFGEGLPGQAAQAGRTLVIEGGRARPGFS
                     LQTGIGEHEPPSVLMVPVLIQDRCPAVLVLAASLEVSPSELAFVESLCVQLGTALSNL
                     KLYDDTRQLAEQLRTRSEEIASKNAQLEAANRMKSEFLANMSHELRTPLNAIIGFSEL
                     LMNGVAGELSAEQREYAGDIFGSGQHLLSLINDILDLSKVEAGAMKLDLAPVNVKSLC
                     MNSVLVVREKAQAEGIRLEVDAADDLGSIDADARKMKQIVYNLLSNAVKFTERGGTVT
                     LAARCVDRAAAGRLSGVRSGRSFPLDNAGSDRFLEIRVIDSGIGIAPEEMGKLFEPFS
                     QLDTGLARRFEGTGLGLTMVKRLAELHGGTVAVESEAGAGASFIVWLPYREAKPDNAA
                     GSGRVHGPQQDGREEADAAAGPWALVVEDDERAAELIRLQLEAEGLAVVRVARAEEAI
                     AILASRTPALITLDILLPGLDGWQFLDRIKAVPELARIPVVIISVVAESNRGLSLGAA
                     AVFEKPVRRDDLHEAIAALGIGPTGGCRAKVLVVDDDPKAVEIIAAHLPSPGFEVVRA
                     YGGAEAIESAHRSVPDLIVLDLMMPEVSGFDVVESLKGNPDTVHIPILIVTARHIAQD
                     EREALSRHVTRIMQKSEFNHGRFIGEVRRALGVQARRQ"
BASE COUNT          564 a         1256 c         1145 g          646 t
ORIGIN      
        1 ggtgatgtag tcgtcgcagc ccgccgccct gcatcgggcc tcgtcaccca tcatggccat
       61 cgccgtcacc gcgatcaccg gtatgcgctg cgtgcgcgga tcggccttca gccttcgcgt
      121 cgcgtccaga cctgagatgc cgggcaactg catgtccatc aggatcaggt cgggctgttg
      181 cgcgaacgcg caggcaagcc cctgttccgc gtcacaggcc tgccacacct ggtggccggc
      241 cttctcgagg atcaggcgaa cgagcttgcg attcaacgcg ttgtcctcga tgaccaggat
      301 cgccgccatc actgcctcct ggcctgtacg ccgagtgccc ggcgcacctc gccgatgaag
      361 cggccgtgat tgaactccga tttctgcatg atgcgggtga catgccggga gagcgcctcg
      421 cgctcgtcct gcgcgatgtg ccgggcggtc acgatcagga tcgggatgtg gaccgtgtcc
      481 ggattgccct tcaggctttc gaccacgtcg aatccgctca cttccggcat catcaggtcg
      541 agcacgatca ggtccggcac ggacctgtgc gcgctctcga tcgcctccgc gccgccatag
      601 gcgcgcacca cctcgaaacc cggcgacggc aggtgcgcgg cgatgatctc gaccgctttc
      661 ggatcgtcgt cgacaaccag caccttggcc ctgcagccgc ccgtcgggcc gatacccagt
      721 gcggcgatcg cctcgtgcag gtcgtcgcgc ctcaccggct tctcgaacac ggccgccgcc
      781 ccgagcgaga ggccgcgatt gctttccgcg acgaccgaga tgatcaccac cggaatgcgc
      841 gcgagttccg gcactgcctt gatccggtcg aggaactgcc agccgtccag tcccggcagc
      901 aggatgtcga gcgtgatcag cgccggcgtg cgcgaagcaa ggatcgcgat cgcctcttcc
      961 gcgcgcgcga cgcgtactac ggccaggccc tcggcttcca gttgcagccg gatcagttcg
     1021 gccgctcgtt catcgtcttc cacgaccagc gcccatgggc cggccgccgc atcggcttcc
     1081 tcgcgcccgt cctgctgcgg cccgtgaacg cgtccgcttc cggccgcatt gtccggcttc
     1141 gcctctcggt acggcagcca gacgatgaag ctcgcgcccg ccccggcctc gctttccacc
     1201 gccactgtcc ctccgtgcag ttcggcaagg cgtttgacca tggtcagccc gaggccggtt
     1261 ccttcgaagc ggcgtgcgag cccggtgtcg agctggctga acggttcgaa cagcttcccc
     1321 atttcctcgg gcgcaatgcc gatgccgctg tcgatcacgc ggatctccag gaagcggtcg
     1381 ctgcccgcgt tgtcgagcgg gaaactgcgt ccggaacgca ctccgctcaa cctgccggcc
     1441 gccgcgcggt cgacacagcg ggccgcgagc gtcacggtcc cgccgcgttc ggtgaacttc
     1501 accgcattcg acagcaggtt gtagacgatc tgcttcatct tgcgggcgtc ggcatcgatg
     1561 ctgcccaggt cgtccgcggc gtcgacttca agcctgatgc cttccgcctg cgccttctcg
     1621 cgcaccacca gcacgctgtt catgcacagc gacttcacgt tcaccggcgc caggtccagc
     1681 ttcatcgcgc ccgcttcgac cttcgacaga tccaggatgt cgttgatcag cgaaagcagg
     1741 tgctggccgc tgccgaagat gtcgcccgcg tactcgcgct gctcggccga gagctcgccc
     1801 gccaccccgt tcatcagcag ctcggaaaag ccgatgatcg cgttgagcgg tgtgcgcagc
     1861 tcgtgcgaca tgttggcgag gaattcggac ttcatgcggt tggccgcctc cagctgcgca
     1921 ttcttgctgg cgatttcttc gctgcgcgtg cgcaactgct ccgcgagctg cctggtatcg
     1981 tcgtacagct tgaggttgct gagcgcggtg ccgagttgca cgcacaagct ttcgacaaag
     2041 gcgagttcgc tgggggaaac ctcgagcgag gccgccagca ccagcaccgc cggacagcgg
     2101 tcctggatca gcaccggcac catcagcacg ctcggcggtt cgtgttcccc gattccggtc
     2161 tgcagcgaga atccgggtcg cgctcgccca ccttcgatca ccagcgtcct gccggcctgt
     2221 gcggcctggc ccggcagtcc ctcgccgaag ccaaattccc tggttgcctc cgcgggcagg
     2281 ccccagccgg ccgcgagcct gaacctgcca gcccagtcgt cgaagtgata cagggcggca
     2341 acgggaaacg gatgcttggt gcacaggagt tcgagcagac cgttgaacat gcggtcgcga
     2401 tcgaagccgg cattgaacag ggccagcgcc tgggtgcgcg tctcgtcgaa acgggccgcg
     2461 atgcgcaggc cgcgctcggt gcgcttgagt tcggtgacat cctggcagac cgccacaccg
     2521 ccggtgatcc gggtgccggc tttgcgcagc ggcacggctg tcacaagcag gctcgcgcgc
     2581 gtgccgtcgg cgctgtcgat ctcgatttcc tcgccgcgca ccgtttcgcc cttgcgaaac
     2641 gcacgcacaa gcggccagtc gtcgccctgc atgcggcgtc cgtccgacac ccgggttccc
     2701 tggaactgcg cataggggtc ggttccctgg tccggcaccg ccgcgcccgg gaacatgtcg
     2761 taaccggccc ggttgatgga cagcctgcca tcggcgtcgg aaatgaagac ggccaccggc
     2821 aagacgtcga gtatctgctt gagccgcgct tcgctctgct cgagcgcctg cgcggccgcc
     2881 tggcgcagcc tggtctggcg ggtgatgcgc gagaacagaa agaccagcac ggcggtcagc
     2941 gccgccgcca gcagggcgag gctgatcgcc gtcgagcgct gggtagcctc ttcctcgagc
     3001 ttgcgctcgt gcagcagccg gcgctcttcg tccatcattc cttcgatcgc gtcctgaagc
     3061 cgcactgcaa gcggatggcc gctgccgaat acctgaactg ccggcatcag tccctggctg
     3121 tcgcggatgc gctgatagtc cagaaaaacc ttgcgccgct ctgcaaccag ttcctccacc
     3181 cgggccaggc gggtcgcctg ggacggattg tcggcaacaa gggtatgaat gcgtgcgatg
     3241 gcggtggccg cgtcgcgcag agattcgtcg cgcaggtcaa gctgttcggc gctgccggtg
     3301 atcacataac tgtggctcgc cgccgacgcg cgccccattg ccgccgcggc ctcgctcagg
     3361 gtgttgagca cttcatgggt gtgaacgacc gacgccgagg ccgccgcgga cagcttcgca
     3421 ctgtgccaga ccacgctgca gagcaggccg aaaaccagaa ggatgagggc gaagcctgcg
     3481 aggagtgttg tttcgactga ttggagacgt tgcatgcggg cgcttcacgg ccgttggaaa
     3541 tcaggccgcc cccacagtag catgcattgc cgtcgggaaa ctttcgctcc cgcaattgca
     3601 ttgatctgaa t
//
LOCUS       DATLGF010000307         3823 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_59948,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000307 DATLGF010000000
VERSION     DATLGF010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59948"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1138
                     /locus_tag="VM406_09385"
     CDS             2..1138
                     /locus_tag="VM406_09385"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HVL76217.1"
                     /translation="MFTEGRGMWLTDHNGKRYLDWLQGWAVNCLGHSPQCVVEAAQAQ
                     VAKLINPSPAFYNEPSIVLANMLTANSCFDRVFFANSGAEANEGAIKLARKWGKVNRN
                     AAGENRYEIITFNHGFHGRTLATMSASGKAGWDTMFAPQVPGFAKAELNDIESVEKLI
                     TDKTVAVMLEPVQGESGVIPATREFMQALRKLTKERNLLLIVDEVQAGMGRCGALFAY
                     QLSNIEPDIMTLGKGIGGGVPLAALLAREEVACFEAGEQGGTYNGNPVMTAIGIAVLS
                     ELLKPGFLGSVQEKGEYLRNALLKLSDRHGLAGERGEGLLRALKLGKDVGPKLVELGR
                     DMEPIGLLLNSPRPDLLRFMPALNVTTQEIDQMIDMLSDLLGRV"
     gene            1421..2569
                     /locus_tag="VM406_09390"
     CDS             1421..2569
                     /locus_tag="VM406_09390"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVL76218.1"
                     /translation="MGKKVVLLLQGGGALGAFQFGAWRALERFLREEEHELIAVAGAS
                     IGAVNAALIARHLHDEEGATPALERFWSRDAPNPPLPFIPFPGEYWRAWNGLLTGLLT
                     GNPALFRPLYQYWTPVGDMLRFWMPLYDVGRSKQTLSAAFGRYRGNAPLLAVAATDVQ
                     SGEAVLFDSAQTHVTPTMLAASTAIPVLFSPVEIGGRYFWDGEIRSESLLPHVLARLH
                     TTLPARDTLQDLLVIVVDMFNARAGGLPSSTISAQYRMLNIILGGKLAFDLEIFKVRN
                     AYVDAVTRLHAAAREHETPPALMQQIEADYRRLLDERIGHIDVVRISRAQFEYEHISR
                     DFDYSPQYLERLTEQGEARATEELREYREGGMQQRPLAKVTPIRSRRQ"
     gene            2566..3507
                     /locus_tag="VM406_09395"
     CDS             2566..3507
                     /locus_tag="VM406_09395"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVL76219.1"
                     /translation="MSPPGSQRVWVLALAALLACAAARAARARTRRRVMLARGELRRL
                     DERQWTIAGRHVIARVAGHPASGAPPVVLVHGLGVSSSYFLPAAERLATRFTVYAPDL
                     PGHGHSEAPAVPLDVRGLADALAQWMQAARIERASIVGHSMGCQVAADFALRHPDRVA
                     RLILIGPTLAPSLRNLLRIAPRFLAGGAFERPALLLHIAKDYLRMGWRVVPEAQSLMR
                     DPIERKLARISVPVMLVCGQRDTLVPQSWLTDAAALARAQDVAVIPGWGHAVQFSAPQ
                     QVVQAITPFLAMPEDTDLEMDRARAGTSEANARSNAP"
     gene            complement(3628..>3823)
                     /locus_tag="VM406_09400"
     CDS             complement(3628..>3823)
                     /locus_tag="VM406_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76220.1"
                     /translation="LPSASCTSCGMPQEVQDALGKMVPFPPRLGKPAEYAHLVKTIVE
                     NVMMNGETIRLDGAIRMQPK"
BASE COUNT          635 a         1275 c         1284 g          629 t
ORIGIN      
        1 cgtgttcacc gagggacgcg gcatgtggct gaccgaccat aacggcaagc gctacctgga
       61 ctggctgcag ggctgggcgg tgaactgcct cggccactcg ccgcagtgcg tcgtcgaggc
      121 cgcccaggcg caggtcgcaa aactgatcaa tccgtcgccg gcgttctaca acgagccgtc
      181 gatcgtgctg gccaacatgc tgaccgcgaa ctcctgcttc gaccgggtgt tcttcgccaa
      241 cagcggcgcc gaagccaacg aaggcgcgat caagctcgcg cgcaagtggg gcaaggtcaa
      301 caggaacgca gccggcgaga accgctacga aatcatcacc ttcaaccacg gcttccacgg
      361 ccgcaccctg gcgaccatgt ccgcttccgg caaggccggc tgggacacaa tgttcgcgcc
      421 gcaggtcccg ggcttcgcca aggccgagct gaacgatatc gaaagcgtcg agaagctcat
      481 caccgacaag actgtcgccg tgatgctcga accggtacag ggcgaaagcg gcgtgattcc
      541 ggccacgcgt gaattcatgc aggcgctacg caagctgacg aaggaacgca atctgctgct
      601 gatcgtcgac gaggtgcagg ctggaatggg ccgctgcggc gcgctgttcg cctaccagct
      661 ctcgaacatc gagccggaca tcatgactct cggcaagggc attggcggcg gcgtgccgct
      721 ggcggcgctg ctcgcgcgtg aggaagtggc ctgcttcgag gctggcgagc aagggggcac
      781 ctacaacggc aacccggtga tgacggccat cggcattgcg gtgctgtcgg aactgctcaa
      841 gcccggcttc ctcggttccg tgcaggaaaa aggcgaatac ctgcgcaacg cgctgctcaa
      901 gctcagcgac aggcacggcc tggctggcga gcgcggcgaa ggcttgctgc gggcgctgaa
      961 gctgggcaag gatgtcggcc cgaaactggt cgagctcgga cgcgacatgg agccgatcgg
     1021 cctgctgctg aactcgccgc gccctgactt gctgcgcttc atgccggcgc tgaacgtgac
     1081 cacgcaggaa atcgaccaga tgatcgacat gctgtcggac ctgctcgggc gcgtctgagg
     1141 cggttcgctt gccaatggtc cgccatgggc cgtcatgccg ttcagattgg catgcatggt
     1201 atcggtcgtc ccggcgaaag ccggggacca ggggcgtcaa tacgtagtac atggaagacg
     1261 cggggccccg gctttcgccg aggcgaccac gtagtcgcga tggcctgact gaacggcact
     1321 accgccacag gccgttccct ttttacccac cgcgctgcaa cttgcccaat ccgccactgt
     1381 ccgtcatgtc tgtcgcattg cacgacaagc ggagtgccag atgggaaaga aagttgtgtt
     1441 gctgctccag ggcggcggtg ccctcggcgc ctttcagttc ggtgcctggc gcgcgctcga
     1501 acgcttcctg cgcgaggaag agcatgaact gattgcggtg gccggcgcat cgatcggtgc
     1561 ggtcaacgcg gcgctgatcg cgcgccatct ccatgacgag gaaggggcca cgcccgcgct
     1621 ggagcgtttc tggagccgcg atgccccgaa ccctccactg ccgttcattc cgtttcccgg
     1681 cgagtactgg cgcgcctgga atggcctgct gaccggcctg ttgacgggca atcccgcgct
     1741 gtttcgcccg ctgtaccagt actggactcc ggtcggcgac atgctgcgct tctggatgcc
     1801 gctctacgac gtcggacgca gcaagcagac gctgtcggcc gcgttcgggc gctaccgcgg
     1861 caatgcgccg ttgctggcgg tcgccgccac cgacgtgcag agcggggagg cagtcctgtt
     1921 cgacagcgcc cagacgcatg tgacaccgac gatgctggcg gcgagcaccg cgattcccgt
     1981 gctgttttcg ccggtcgaga tcggcggacg ctacttctgg gacggcgaga tccgcagcga
     2041 gagcctgctg ccccacgtgc tggcgcgcct gcacaccacg ctgcctgcgc gcgacacact
     2101 gcaagacctg ctcgtgatcg tggtcgacat gttcaacgcg cgcgctggag gcctgccctc
     2161 cagcacgatc tctgcccagt accgcatgct gaacatcatc ctcggcggca agctggcgtt
     2221 tgacctggaa atcttcaagg tgcgcaacgc ctatgtcgac gccgtcacgc gcctgcacgc
     2281 cgccgcacgc gagcatgaga cgccccccgc gctgatgcag cagatcgaag ccgactaccg
     2341 caggctgctc gacgaacgca tcggccatat cgatgtggtg cgcatcagtc gcgcccagtt
     2401 cgagtacgaa cacatttcgc gcgatttcga ttattcgccg cagtacctcg agcgcctgac
     2461 cgagcagggc gaggcgcgcg caacggaaga actgcgcgag taccgcgaag gcgggatgca
     2521 gcagcgtccg ctcgccaagg tcacccccat ccgcagcagg cggcaatgag tccgcccgga
     2581 agccaacggg tatgggtgct cgcgctggcc gccctcctcg cttgcgccgc ggctcgtgca
     2641 gcgcgtgccc ggacccgccg gcgcgtgatg ctggcacgcg gggagctgcg ccggctcgat
     2701 gaaaggcaat ggacgatcgc gggccggcac gtcattgcgc gggtcgcggg gcatccggca
     2761 tcgggcgcgc cgccggtcgt tctcgtgcac gggctgggcg tgtcgagctc ctatttcctg
     2821 ccggcggcgg agcggctggc cacgcgcttc accgtctacg cacccgacct tccgggacac
     2881 gggcacagcg aagcgccggc cgtcccgctc gacgtgcgcg gcctggccga cgcgctggcg
     2941 cagtggatgc aggccgcgcg catcgagcgc gccagcatcg tcggccattc gatgggttgt
     3001 caggtggcgg cagacttcgc gctacggcat ccggaccggg tggcgcgcct catcctgatc
     3061 gggccgacgc tggcgccgtc gctgcgcaac ctgctgcgca tcgcaccgcg ctttctcgcc
     3121 ggcggcgcat tcgagcggcc ggcgctgcta ctccatatcg cgaaggatta cctgcgcatg
     3181 ggctggcgcg tggtgcccga ggcgcaaagc ctgatgcgcg acccgatcga gcgcaagctt
     3241 gcgcgtatct cggtaccggt gatgctcgta tgcggccagc gcgacacgct ggttccgcag
     3301 tcgtggctca cggacgccgc cgcactcgcc cgcgcgcaag acgtcgccgt cattcccggc
     3361 tggggacatg cggtgcaatt cagcgcgccg caacaggtcg tccaggcgat cacgccgttt
     3421 ctggcgatgc cggaggacac ggacctggaa atggatcgtg cgcgcgcggg cacgagcgaa
     3481 gcgaatgcgc gcagcaatgc gccatgagcg acgctgagaa gatgccgctt gccgcgtcgc
     3541 gccggcgcgg gttgccttcg gcatctgtcg tgggaggaat gcggcggggc ctgccgttgc
     3601 cggtaccccg ccccgccgtg cgcggactta cttcggctgc atgcggatcg cgccgtccag
     3661 gcggatcgtc tcgccgttca tcatcacgtt ctcgacgatg gtcttgacca ggtgcgcata
     3721 ctcggccggt ttgcccaggc gcggcgggaa cggaaccatc ttgccgagcg catcctgcac
     3781 ttcctgcggc atgccgcagg aagtgcagga tgcgctcggc aag
//
LOCUS       DATLGF010000308         4072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_59954,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000308 DATLGF010000000
VERSION     DATLGF010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59954"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="VM406_09405"
     CDS             complement(<1..1009)
                     /locus_tag="VM406_09405"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HVL76221.1"
                     /translation="MSGRQKAAVADAKPMLFVDNRPLLRRPGAPAACGAQASGIRFVG
                     LDVGSTTVKAVVMQGGRSIWRDYKRHGTRQAEMVLAFLERIEAECGLEPGTDRIYLTG
                     SGAGGLAPLIGGKCVQEVVAVAAAVEKLHPDVRFVSEIGGEDMKTVFFTPAGNGNSKQ
                     VYMQSACSGGTGTFVEKTARKLGIATEDLALMPYEGMNLHKVSSKCGIFAEADANTLV
                     KSGVPVEEIIASLYEAVVYQNLASLTRGNTPLPRVLLLGGPNLFFKGLQQAWQHHLHK
                     LWNERRVEFPADAAPESLILVPEDALYYACLGCAEVGAADHAGSALYSGTERLCWWID
                     VG"
     gene            complement(1006..2550)
                     /locus_tag="VM406_09410"
     CDS             complement(1006..2550)
                     /locus_tag="VM406_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76222.1"
                     /translation="MSQTESILHYRAYKPRPFTAEERDRVTILFGGLHWRAERLLQGS
                     IENCGYKARVLPPATKQDLLTGRETADIGQCCPTSFTTGNLANFLREEAKRKGAKAVE
                     DEYVYVTAGACGACRFGQYHQSYELSLRNIGMESFRMFLLDQDVSDQGTKHGGGLDIN
                     LPFTLGAAWAMLVADVLQDMEYQIRPYEVRPGQTNEVVKESVEYLYEVFRNRPYRKGK
                     WDAVLWHMATPYFANALREVRRRFEQIEVDRLRVKPTVKITGEFYLQTVEGDPNYNIQ
                     HWLEREGAEVYPAAVMVWLDYLLRLAAQQQDDRAHVQRGARWRARAIRTGQRLLRWNH
                     DRMRGALGGIPREMPEQNELRDLAAPYYHSRLDGGEGDMLIGKALWAYKHKKAHMTCE
                     LSPYSCMPNTMSIGAMAAVLGKYPDLLYAPLEVKGDAEVHALSRCQMILTEAKKQAQR
                     EYEEALEQTGLTTERARELLDAAPQFRRATFRLPHYGMAGAAANMVMHLAREQGARRG
                     AARRSR"
     gene            2705..3475
                     /locus_tag="VM406_09415"
     CDS             2705..3475
                     /locus_tag="VM406_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HVL76223.1"
                     /translation="MAAASSAPAASRKVLTVNTHKGFTALNRRFVLHELREAVRTVGA
                     DIVFLQEVLGKHVAHQMRFANWPDAPQYEFLAESIWPSYAYGCNAVYPHGHHGNALLS
                     KWPILRHQNHDISASDPEKRGLLHCVLHAPDSGVHLHAICVHLALWDKHRRPQTALLC
                     DLLATEVPSDAPLIVAGDFNDWRLRAHAQLVREAKLREVFVEAFGEPARTFPARWPLL
                     RNDRIYVRNANAHAPMVLPRRPWTHLSDHTPLAAEIDL"
     gene            3472..>4072
                     /locus_tag="VM406_09420"
     CDS             3472..>4072
                     /locus_tag="VM406_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HVL76224.1"
                     /translation="MKNGWKKGNRVVLLENGEAYYPRVFDAIAGARFQILIETFILYE
                     DKIGLALKDVLLDAVARGVAVTVRVDGWGSPPSKLSEHYIRELADAGVNFQVYGELSW
                     ISKRLHLARRLELFRRMHRKMVVIDGRLGYIGGINFSADHLADYGPESKQDYAVELTG
                     PIVDDLHRFLEWEGTAPAGLRARWRRWRTAREVRAPNPPS"
BASE COUNT          680 a         1377 c         1266 g          749 t
ORIGIN      
        1 ggcccacatc gatccaccag cacagtcgtt cggtgccgct gtagagcgcg gagccggcat
       61 gatccgccgc gccgacttcg gcgcaaccga ggcaggcgta gtacagcgcg tcctcgggca
      121 cgagaatcag cgattccggc gccgcatcgg ccggaaactc gacacggcgc tcgttccaca
      181 gcttgtgcag gtgatgttgc caggcctgct gcagcccctt gaagaacagg ttcggcccgc
      241 ccagcagcaa cacgcgcggc agcggcgtat tgccgcgcgt gagcgaagcg aggttctggt
      301 acaccacggc ctcgtagaga ctggcaatga tttcctccac cggaacgccc gatttcacca
      361 gcgtgttggc gtcggcctcg gcgaagatgc cgcacttgga gctgaccttg tgcaggttca
      421 tgccttcata cggcatcagc gcgagatcct ccgtcgcaat gccgagcttg cgcgcggtct
      481 tctcgacgaa ggtgccagtg ccgccgctgc aggccgactg catgtagacc tgcttgctgt
      541 tgccgttgcc cgccggcgtg aagaaaacgg tcttcatgtc ctcgccgccg atctcggaga
      601 cgaagcgcac atcgggatgc agcttttcga cggcggcggc aaccgcgacc acttcctgca
      661 cgcacttgcc gccgatcagc ggcgccagtc cgccggcgcc ggagcccgtc aggtagatgc
      721 ggtcggtgcc cggctccagt ccgcattccg cttcgatgcg ctccagaaac gccagcacca
      781 tctcggcctg gcgcgtgccg tggcgcttgt agtcacgcca gatgctgcgc ccgccttgca
      841 tcacgaccgc cttcaccgtg gtcgatccca catcgagtcc gacgaagcga atgccggatg
      901 cctgcgcccc gcaggcggcg ggtgcgccgg gacgtcgcag cagcgggcga ttgtcaacga
      961 acagcatcgg cttcgcgtcg gccaccgccg ccttctgccg cccgctcatc gactcctccg
     1021 tgccgcaccc cgccttgcac cttgttcgcg tgcaagatgc atcaccatgt tggccgcggc
     1081 gccagccatg ccgtagtgcg gcagcctgaa agtcgcgcgc ctgaattgcg gcgcggcatc
     1141 gagcagttcg cgcgcccgtt cggtggtcaa tccggtctgc tctagcgcct cctcgtattc
     1201 ccgctgcgcc tgcttcttcg cctcggtcag gatcatctgg cagcgcgaca gcgcatgcac
     1261 ctcggcgtcg cccttgacct caagtggcgc gtacagcagg tccggatact tgccgagcac
     1321 cgccgccatc gcaccgatgc tcatcgtgtt cggcatgcag gaatacggcg acagctcaca
     1381 ggtcatgtgc gccttcttgt gcttgtaggc ccacagggcc ttaccgatca gcatgtcgcc
     1441 ctcgccgcca tcgaggcggc tgtgataata cggagcagcg aggtcgcgca attcgttctg
     1501 ctccggcatt tcgcgcggga tgccgccgag cgcgccgcgc atgcggtcat ggttccagcg
     1561 cagcaggcgc tgtcccgtac gaatggcgcg ggcccgccag cgggcaccgc gctgcacgtg
     1621 ggcgcgatcg tcctgctgct gcgcggcgag gcgcaacagg tagtcgagcc agaccatcac
     1681 cgcggcgggg tagacttccg ccccttccct ctccagccag tgctggatgt tgtagtttgg
     1741 atccccctcg accgtctgca ggtagaactc gccggtaatc ttgacggtcg gcttcacgcg
     1801 cagccggtcc acctcgatct gctcgaaccg gcgccgaact tcgcgcagcg cgttcgcgaa
     1861 atacggcgtg gccatgtgcc acagcaccgc gtcccacttc cccttgcgat acgggcggtt
     1921 gcggaacact tcgtacaggt attcgaccga ttccttcacg acttcgttgg tctgtcccgg
     1981 ccgcacctcg tacgggcgga tctggtattc catgtcttgc agcacgtcgg cgaccagcat
     2041 cgcccatgcg gcgccaaggg tgaacggcag attgatatcc agtccgcctc cgtgcttggt
     2101 tccctggtcc gagacgtcct gatcgagcag gaacatgcgg aacgattcca tgccgatgtt
     2161 gcgcagcgac agctcgtagc tctggtgata ttgcccgaag cggcaggcac cgcaggcgcc
     2221 tgccgtcacg tacacgtatt cgtcctcgac cgccttcgca cccttgcgtt ttgcctcctc
     2281 gcgcaggaag ttggcgagat ttccggtagt gaagctggtc ggacagcatt ggccgatgtc
     2341 ggccgtctcg cgaccggtga gcaggtcctg tttggttgcg ggaggaagga cgcgcgcctt
     2401 gtagccgcag ttctcgatcg atccctgcag cagccgctcg gcgcgccagt gcaggccgcc
     2461 gaacaggatc gttacccggt cgcgctcctc cgccgtgaac ggcctcggct tgtaggcgcg
     2521 gtaatgaagg atcgattcag tctggctcat gctcttcctc cggctgtcca atggtgggga
     2581 caactgcgtg gacaactgcg tgcgaatcaa gcaagcctag caaccgctca ccataaatcg
     2641 gttatgaaaa atcaaagcgg gctgcgccgc gcctgattgg cacgttccct gcaagcagag
     2701 caacatggcc gccgcatcct ccgctcccgc cgcgtcccgc aaggttctta cagtcaatac
     2761 acacaagggc tttacggcgc tcaatcgtcg gttcgtgctg cacgaactgc gggaggcggt
     2821 gcgcaccgtg ggcgcggaca tcgtgttcct gcaggaggtg ctgggcaagc acgttgcgca
     2881 ccaaatgcgc ttcgccaact ggcccgacgc gccgcagtat gaattccttg cagagtcgat
     2941 ctggccctcg tacgcgtacg gctgcaatgc ggtctatccg cacggccacc acggcaacgc
     3001 cttgctgtcc aagtggccga tcctgcgtca ccagaaccac gacatatcgg ccagcgatcc
     3061 cgagaagcgc ggtctgctgc actgtgtcct gcacgcgccc gattccggtg ttcacctgca
     3121 tgcgatctgc gtgcacctcg cgctgtggga caaacatcgc aggccgcaga ccgccctgct
     3181 gtgcgatctg ctcgcgaccg aagtgccgtc tgacgctccg ctgatcgtgg ccggtgattt
     3241 caacgactgg cgtctgcgcg cgcacgcgca actggtgcgc gaagcaaagc tgcgcgaggt
     3301 gttcgtggaa gccttcgggg aaccggctcg cacgtttccg gcacgctggc cgctgctgcg
     3361 caacgacagg atctacgtgc gcaacgccaa cgcccatgca ccgatggtgc tgccgcgccg
     3421 gccctggacc cacctctcgg atcacacccc gctagccgcg gagatcgacc tatgaagaac
     3481 ggctggaaga aaggcaatcg cgtcgtgctg ctggaaaacg gcgaagcgta ctatccgcgt
     3541 gtgttcgacg cgatcgccgg cgcccgcttc cagatcctga tcgagacctt catcctgtac
     3601 gaggacaaga tcgggttggc gttgaaggac gtgctgctcg acgcggtcgc acgcggcgtc
     3661 gccgtgacgg tccgggtgga cggctgggga tcgccgccgt ccaagctcag cgagcattac
     3721 atacgcgagc tggcggacgc gggcgtgaac ttccaggtgt acggggagct gagctggatt
     3781 tccaagcgcc tgcacctggc gcgccgcctc gaactgttca gacgcatgca ccgcaagatg
     3841 gtcgtcatcg acggcaggct gggttacatc ggcggcatca atttctcggc cgaccatctt
     3901 gccgattacg gtccggaatc caagcaggac tacgcggtcg aactcacggg cccgatcgtc
     3961 gacgacctgc accgctttct cgagtgggaa gggactgctc ccgccggcct gcgtgcgcgc
     4021 tggcgccgct ggcgcacagc gcgcgaggtg cgcgcgccga atccgccatc cg
//
LOCUS       DATLGF010000309         2428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_228819, whole genome shotgun sequence.
ACCESSION   DATLGF010000309 DATLGF010000000
VERSION     DATLGF010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228819"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..1200
                     /gene="rodA"
                     /locus_tag="VM406_09425"
     CDS             79..1200
                     /gene="rodA"
                     /locus_tag="VM406_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HVL76225.1"
                     /translation="MNTAHLNQRRALWPTVKPYIAVFDGTLALIVFLLVAVGLVTMYS
                     AGMDAPGRFEGQIRNILVAFVVMWMAALIPPQTLMRFAVPLYVAGVALLVAVAMFGIV
                     KKGARRWIDLGIVIQPSEIMKIAVPLMLAWFFQKREGFIRWQEYLIAAILLMIPMGLI
                     LKQPDLGTSLLVGTAGFAVIFFAGLSWKILFTLGAAGLASLPVIWSMLHDYQRGRVMM
                     LLDPTTDPLGRGFHIIQSTIAIGSGGVSGKGWLNGTQTHLDFIPERTSDFIFAVYSEE
                     FGLIGNGVLLLLYLFLIGRCVLIAANAPTLFARLLAAAITTIFFTYAFVNMGMVSGIL
                     PVVGVPLPFISYGGTAFVTLGLGLGMLMSIQRHRKLIQS"
     gene            1322..2416
                     /locus_tag="VM406_09430"
     CDS             1322..2416
                     /locus_tag="VM406_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HVL76226.1"
                     /translation="MQYQGRRTAGKRLGWMCAAAVFTLAGCSSVPLTSDTTPPSPLPT
                     PAPTSAPAPVARADLPNLPPAGSGRGGYYLDDGPGDNPPDGLHDIPDAEPRIEPYTTR
                     GNRPYVVFGKNYTPFTDDRPYKARGRGSWYGKKFHGQTTSSGEPYDMYKMTAAHPTLP
                     LPSYVRVTNLENGRQVIVRVNDRGPFVAGRIIDLSYTAALKLGYLTKGSALLEVERLL
                     PDEIRRMAQERERAAPPAVLATSGGAETAEAAEVAQPDMPERVALASEAVFVQTPAQP
                     VLVPAASTPMTGSGIFLQLGAFGQAANAAAARQRFGQQSGLALPPIEVVEQGGLFRLF
                     SGPFASRADAEAAAMLLQRSIQVSPLIVQR"
BASE COUNT          378 a          850 c          774 g          426 t
ORIGIN      
        1 cgccgccgcg ccggcgcgtg tcgccgccac gtcgtccgcc cggccggcac agcccgcatc
       61 cgcacccgga gccgcccaat gaatacggca cacctgaacc agcgccgcgc gctctggcct
      121 accgtcaagc cctacatcgc cgtgttcgac ggcacgctgg cgctgatcgt gttcctgctg
      181 gtggccgtgg gcctggtcac catgtattcg gccggaatgg atgcgcccgg acgtttcgag
      241 ggccagatcc gcaacatcct ggtcgccttc gtcgtgatgt ggatggcggc cctgatcccg
      301 ccgcagaccc tgatgcgctt tgcggtgccg ctgtatgtgg ccggggtggc cttgctggtc
      361 gcggtcgcga tgttcggcat cgtgaagaaa ggcgcgcgcc gctggatcga ccttggcatc
      421 gtgatccagc cgtccgagat catgaagatc gccgtgccgc tgatgctggc ctggttcttc
      481 cagaagcgcg agggcttcat ccgctggcag gagtatctga tcgcggcgat cctgctgatg
      541 ataccgatgg gcctgatcct caagcagccc gacctcggca cgtcgctcct ggtcggcacc
      601 gccggctttg cggtgatctt cttcgccggc ctgtcgtgga agatcctgtt tacgctgggc
      661 gcggccggat tggccagcct gccggtgata tggtcgatgc tgcacgacta ccagcgcggc
      721 cgcgtgatga tgctgctcga tccgaccacc gacccgctcg ggcgcggctt ccacatcatc
      781 cagtcgacaa tcgcgatcgg ctccggcggc gtgtccggca agggatggct gaacggcacc
      841 cagacgcacc tggacttcat ccccgagcgc accagcgact tcatcttcgc cgtgtattcg
      901 gaagaattcg gcctgatcgg caacggcgta ctgctgctgc tctacctgtt cctgatcgga
      961 cgctgcgtgc tcatcgccgc caatgcgccg acgctgttcg cacgcctgct cgcggcggcg
     1021 atcaccacca ttttcttcac ctatgcgttc gtcaacatgg gcatggtcag cggcatcctg
     1081 ccggtcgtcg gcgtgcccct gccattcatc agctatggcg gcaccgcctt cgtcacgctc
     1141 ggcctcgggc tcggcatgct gatgagcatc cagcgtcatc gcaagctgat acaaagctga
     1201 cgcctcgaca gtgtgacaac gcgcaacatt aggttgcggc aacgcatgtc tcgctgcaaa
     1261 ctgtttcctt ctttcaagcc ggccgtaatc ggccggttgc catcgacaag aggggaacgg
     1321 catgcagtat cagggaaggc ggacggcggg caagcgcctg gggtggatgt gcgcggcggc
     1381 ggtatttacg ctggcggggt gcagcagcgt cccgctgacc tcggacacca cgccgccaag
     1441 tcctcttccc actcctgctc ccacgtccgc gcccgctccg gtcgcccgcg cggacttgcc
     1501 gaacctgccc ccggccggct ccgggcgcgg cggctactac ctggacgatg gccccggcga
     1561 caatccgccc gacggtctgc atgacatccc cgacgccgaa ccgcgcatcg aaccgtacac
     1621 cacgcgcggc aatcgtccct atgtcgtttt cggcaagaac tacacgcctt tcaccgacga
     1681 ccgcccgtac aaggcacgcg ggcgcggaag ctggtacggc aagaagttcc acggccagac
     1741 cacctcctcg ggcgaaccct acgacatgta caagatgacg gccgcgcatc cgacgctgcc
     1801 gctgccgtcc tacgtgcgcg tgacgaatct ggagaacggc cgccaggtga tcgtgcgcgt
     1861 gaacgatcgc ggacccttcg tcgccggacg catcatcgat ctgtcttaca cggccgcgct
     1921 caagctcggc tacctgacca agggcagcgc gctgctcgag gtcgagcgcc tgctgccgga
     1981 cgagatccgc cggatggcgc aggagcgcga acgcgccgcg ccgccggctg tgcttgccac
     2041 ctcgggcggc gccgagaccg cggaggcggc cgaagtcgcc cagccggaca tgcccgaacg
     2101 cgtggcactg gcaagcgaag cggtgttcgt gcagacgccg gcacagcctg tgctcgtccc
     2161 ggccgcgtcc acgccgatga ccggctccgg catcttcctg cagctcggcg cattcgggca
     2221 ggcggcgaac gcggcggccg cgcgccagcg cttcggccag cagtccggac tcgcgctgcc
     2281 gccgatcgag gtggttgagc aaggcgggct gttccggctt ttcagcggcc cgttcgcgag
     2341 ccgggccgac gccgaagctg cggcaatgct gcttcagcgc agcatccagg taagcccgct
     2401 gatcgtgcag cggtagttgg ttcagcct
//
LOCUS       DATLGF010000310        16538 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_212661, whole genome shotgun sequence.
ACCESSION   DATLGF010000310 DATLGF010000000
VERSION     DATLGF010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 16538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16538
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212661"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /gene="ilvC"
                     /locus_tag="VM406_09435"
     CDS             complement(<1..747)
                     /gene="ilvC"
                     /locus_tag="VM406_09435"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HVL76227.1"
                     /translation="MKVFYDKDCDLSLIKGKNVTIIGYGSQGHAHALNLNDSGCKVTV
                     ALRKGGASWEKAKNAGLNVMEVIDAVKQADVVMILLPDENIAQVYKENVEPHAKQGAV
                     LAFAHGFNVHYGQVVPRADLDVIMVAPKAPGHRVRELYQAGAGTPGLVAVHQDASGAA
                     LDLALAYGTAIGCGRVGLLETTFAEETESDLFGEQAVLCGGATQLIKMGFETLVEAGY
                     QPEIAYYECLNELKLIVDLIYEGGIANMNYS"
     gene            complement(783..1274)
                     /gene="ilvN"
                     /locus_tag="VM406_09440"
     CDS             complement(783..1274)
                     /gene="ilvN"
                     /locus_tag="VM406_09440"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HVL76228.1"
                     /translation="MRHIISVLLENEAGALSRVVGLFSARGYNIETLTVAPTEDPTLS
                     RMTIVTSGSDDVIEQITKHLNRLIEVVKVVDLTEGAHIERELMLIKVRAVGKEREEMK
                     RTADIFRGRIIDVTEKTYTIELTGNKSKLDAFIESIDRTAILETVRTGGSGIGRGERV
                     LKI"
     gene            complement(1277..2992)
                     /locus_tag="VM406_09445"
     CDS             complement(1277..2992)
                     /locus_tag="VM406_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HVL76229.1"
                     /translation="MSAELTGAEIVVRCLAEEGVEHVFGYPGGAVLYIYDAIFNQEKF
                     QHILVRHEQAAIHAADAYSRSSNKVGVAIVTSGPGVTNAVTGLATAYMDSIPMVVISG
                     QVPSHAIGQDAFQECDTVGITRPCVKHNFLVKDVKELAATMKKAFFIATTGRPGPVLV
                     DIPKDITMHKCAFEYPKEVDMRSYKPVDKGHSGQIRKAVQLLLSAERPMIYTGGGIIL
                     ANAAPELNKLVDKLGYPCTNTLMGLGGYRASSDKFVGMPGMHGTYEANMAMQHCDVLI
                     AIGARFDDRVIGNPKHFATHPRKIIHIDIDPSSISKRVKVDIPIVGNVKDVLVELLSQ
                     LDAAEVKPNAQALAGWWRQINEWRGRECLKYSTSNEVIKPQEVVEKLWQVTKGDAFIT
                     SDVGQHQMWAAQYYDFDKPRRWINSGGLGTMGVGLPYAMGVQMANPDATVACITGEAS
                     IQMCIQELATCKQYHLTPKIILLNNRFLGMVRQWQQIDYGSRYSESYMDSLPDFNKLA
                     EAFGHVGMKIEKPGDVEGALKEAFGMKDRLVFMNFITDQTENVWPMVKAGKGLTEMLL
                     GSEDL"
     gene            3478..3999
                     /locus_tag="VM406_09450"
     CDS             3478..3999
                     /locus_tag="VM406_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HVL76230.1"
                     /translation="MTTLASPIPIPAPSIKRRLASMLYEAMLLFGVVAFAGAVFAVML
                     EQKHALYLRHQLQYVLFFVLGLYFIWFWTHGGQTLAMKTWRVRLVDASGKAVGAGRAL
                     LRYVLAWAWFLPGLALAAAVGAEGSLMLLIPATNFALWALTPFLDPQRQFLHDRLAGT
                     RLVVVEPAPPRKS"
     gene            complement(3996..4694)
                     /locus_tag="VM406_09455"
     CDS             complement(3996..4694)
                     /locus_tag="VM406_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76231.1"
                     /translation="MDRHTTGFKLRRELERVTRARKAAAADPALSARRLALRRFQADR
                     IARTHSDLLAASDSAPAARFFLTDLYGSHDLTERDANLARIVPTMERMLPEAALHTIA
                     EAVALDALSEKLDRIMAEALGVDFSEADYVAVFRSLTSTHERRLQLAHMDSVGRALCQ
                     LVRVPLIGSTLRVMRGPARLGQLMQLQEFLERGYHAFKGMRHPERFVGTVVERERKLM
                     ERIYAGVEDPFAIH"
     gene            4909..5904
                     /locus_tag="VM406_09460"
     CDS             4909..5904
                     /locus_tag="VM406_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVL76232.1"
                     /translation="MIAWLTRLLLILQAAIATALYLLFTRTLGIQNVLLAAAMAIAAV
                     PAVRILIIANNFMLAHRFRSPLPEHCKLDWRGRLALFLGEVRASLTASSWHLPFKRFH
                     KRPIAGTRELPVLLVHGYGCNSGYWAPFSVALSRAGITHHALDMEPVFGSIDGYAAHI
                     HAAVEELCRDSGQERIVIVAHSMGGLAVRAYLRAHGSTRVAKVITLGTPHRGTALARL
                     GAGINSQEMLWAVREQEGLCSKWLRELAASETEQTYRLFVSIYSHHDNIVSPQTSSHL
                     PGARNIALHGIGHVALAPTPRVMELVIGEIKTLAADEQRDPSRPGTLRDKVHAAG"
     gene            5979..6653
                     /locus_tag="VM406_09465"
     CDS             5979..6653
                     /locus_tag="VM406_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVL76233.1"
                     /translation="MQQKAPRRTRERILELSLRLFNEFGEPNITTTVIAEELSISPGN
                     LYYHFRNKDDIVNSLFSQFETEIGRMLSVPEGRRPTMEDVWLYLHLSFELVWRYRFFY
                     RDLSDLLSRNRKLELHFKQILSHKTKVARQLCLGLRADGAMDASDEEIEALSTNMVVV
                     ATYWLSYEYVRNPRKYTEQQAMSEALVRGCYQVLSLIGPYLRGETHVLFEKLAAEYLT
                     KLRQAP"
     gene            6802..7395
                     /locus_tag="VM406_09470"
     CDS             6802..7395
                     /locus_tag="VM406_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HVL76234.1"
                     /translation="MKTLCIYCGSRMGASPAYATAARRMAAAMVASGVSLVYGGGKVG
                     LMGVIADEVLRLGGEAVGVIPEALMAREVGHTGLTRLHVVRDMHERKALMAELADGFV
                     AMPGGIGTFEELFEVYTWAQLGLHDKPIALYNAAGYYDGLIAFLEHVVQQGFLNRDQA
                     ALLLHGEDAADLVQRLRAFVAPPHEKALPSDQARRII"
     gene            complement(7388..8569)
                     /locus_tag="VM406_09475"
     CDS             complement(7388..8569)
                     /locus_tag="VM406_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76235.1"
                     /translation="MSLINQMLKELDARRSEPGGASPFGGHVRAVPQRRGPHPAWWVA
                     LGAFLGLGAVVAWFILRPVAQPYLVQAQLPLKMDVGLNPVAPARPDEPVASAPATLPV
                     ASAPATLPVASEERPAAVLPPSAEAEPAPTLAAMTAADAAAQARVPSAPAAPAAFPPS
                     PAAPPSPLRPAAALPAAHRAEPVAAPVDSRVPAQPITKQVQEPTRAQRAENEFRKATV
                     ALQHGRRADAENGFEQALILDAGHAAARQALIALLLEGKNVDEAVRLAREGLEQDARQ
                     TGFAMILARLQVEKGELKPAIDTLQRSRQHATERADYIAFLAALLQRAERHAEAIEQY
                     AQALQRTPQNGVWWMGLGISLQAEQRRGEAMEAFARAKASQSLSPDLVAFVETRLAQL
                     K"
     gene            complement(8566..9459)
                     /locus_tag="VM406_09480"
     CDS             complement(8566..9459)
                     /locus_tag="VM406_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVL76236.1"
                     /translation="MYKTYFGLRELPFGITPDTSFFFACTTYQEALNTLLVAVKNGEG
                     FIKITGEVGTGKTLLCRKFMASLDKGFVTAYVPNPYLEPRALMLALADELEVSVAPEA
                     DQHQLLKAINYRLLILAREGRRPVLCLDEAQAMPIESLEALRLLTNLETEKRKLLQIV
                     MFGQPELDRKLQQEAIRQLNQRITFHYHLGSLGRDDMASYLAHRLTVAGYSGGRLFSG
                     SAIRALHTASGGTPRLINVLAHKSLMLCFGEGKQEVAARHVKAAARDTGAARRAWWPW
                     AWAASAVVLVGLSGIGWMVLK"
     gene            complement(9463..11202)
                     /gene="mshL"
                     /locus_tag="VM406_09485"
     CDS             complement(9463..11202)
                     /gene="mshL"
                     /locus_tag="VM406_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus (MSHA type) biogenesis protein MshL"
                     /protein_id="HVL76237.1"
                     /translation="MQGTMVRKATIILVAASLAACAAPAKRDTYDMISAELGNAAQLA
                     QQPAAPDAVSAALLPPLQIEIPKPTQPAEERFNVTFNNVPADQFFMAIVSGTRYNMLV
                     HPDVTGTISANLRDVTVLEALDAIRELHGFDYRVDGNRITVRPASLQTQVFQVNYLTG
                     NRRGTSELRVTSTSVADAGTANTATGIVNQLGQPAQAPGQTTAIARDSSKVSTESDSD
                     FWSELRLALEAIVGVGREGRSVVISPQSGVVVVRGMPDELRNVGAFLKATQASVARQV
                     ILEAKILEVQLNEAYQTGINWAAFRTSANSPASFGLLSPGTALAPLGAGNVPATVASP
                     GSTGVAATPGSAIGALTSATGSLFGLAFQTSNFSALISFLESQGNVHVLSSPRIATLN
                     NQKAVLKIGTDEFFVTNVSTTTTTGNATVTTPSVTLQPFFSGVVLDVTPQIDERGFII
                     LHVRPSVSQVDTVTKSIDLGTGGSLRLPLAASNTSETDSIVRGRDGQIVAIGGLMRHA
                     SRNDRSQIPGVGSAPGIGALFGNTNQVSQKRELVILLKPTIVQDDTGWAQDILDSQQR
                     IQRLAPREEPLRW"
     gene            complement(11202..11597)
                     /locus_tag="VM406_09490"
     CDS             complement(11202..11597)
                     /locus_tag="VM406_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSHA biogenesis protein MshK"
                     /protein_id="HVL76238.1"
                     /translation="MAAHLIRFGQAVLAAACSMPVVGLCQSLPDPTRLPSALGQGRVE
                     NAVAAPVADALALQSILISPQRRLAVIGGRTVAVGDAVGNARVLRIQETEVVLREGSE
                     TRILRMYPEVEKKSPVAAQALPANKNKQQ"
     gene            complement(11582..12250)
                     /locus_tag="VM406_09495"
     CDS             complement(11582..12250)
                     /locus_tag="VM406_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MSHA biogenesis protein MshJ"
                     /protein_id="HVL76239.1"
                     /translation="MKRHWEKFSARVDALTLRERVIIFLMAALALVTAMNMLALDPKD
                     AEQKRIARHMQDQQAQIAQLRADIARKVAESTADPDADARQRLQLLQQQKAGLQESLA
                     GMQRGLVSPDRIAPLLENILSRNTRLRLVSLKKLPVVNLARTHGEEMVDAGLRETGLY
                     RHGVELTVEGGYLDMLNYLTELESLSTQLFWGDLAFTVENHPNASMTVTVYTLSLDKR
                     WLHI"
     gene            complement(12247..12897)
                     /locus_tag="VM406_09500"
     CDS             complement(12247..12897)
                     /locus_tag="VM406_09500"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HVL76240.1"
                     /translation="MSQQINLFNPIFLTQKKYFSAATMLQALGLILLGSGLMVAYASV
                     QLAQMRAEANAVTAQLEAAEAQLAAINAAHGTRQKDGLLQADLRRVEAELIALERLAQ
                     SLSRGDNRQPGGYSEYMRAFSRQIVDGVWLTGFSIRDGGNEIGLSGGALRPELLPAYL
                     NRLGREPSMRNKSFDMLEMGAPAAAARSSAQAPAFVEFRLQSSERPAPASAGRELQ"
     gene            complement(12894..13916)
                     /locus_tag="VM406_09505"
     CDS             complement(12894..13916)
                     /locus_tag="VM406_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agglutinin biogenesis protein MshI"
                     /protein_id="HVL76241.1"
                     /translation="MHVIPLTPSGYYPKFGAFLRCHKAMRFFAKSKTEQGWLALLIER
                     GAVLAVHVVRPAGGRPCVQLAASFPCEPGSEGAVLDRVAKELHVQRFRCTTMLGAGDY
                     RLITVEAPNVPVDELKIAIRWRLKDMLDFHVDDATIDVLSIPSDTNAPVRGQSMYAVA
                     ARNQVIQQRQRAFEQAKIPLGVIDIPELAQRNLAALAETEGRGLAMLTFGPDGGLLTV
                     NHAGELCLSRRIDIAFEQLLHADATQKAALLERVTLELQRSLDHVERQFQFMSLSKLL
                     IGPLGDDDAGLQEHLSANLYVPVERFRLESVTDLERVPALLAPEVQRQYLLAVGAGLR
                     QEEKVL"
     gene            complement(13959..16409)
                     /locus_tag="VM406_09510"
     CDS             complement(13959..16409)
                     /locus_tag="VM406_09510"
                     /inference="COORDINATES: protein motif:HMM:NF042738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HVL76242.1"
                     /translation="MPNVPVADLAIEMTRIDSLRLGTNGSYLITVGNNGPNEEPGPIT
                     VTNTLPPQLAYVSAGGAGWSCSASGQTVTCVYSGAALAPGTYAPALTLTVSVTSASTS
                     VTNVATVSGARFDNVSENNSASDTASVLVPGYVFTDSPCSHNVAFGSPGQCTIMNWGP
                     QTAAQALDGIYITNLNNSGVPTRLHASQVRTETMQFALTCHNPATNAGVRAAFSAVSA
                     ALPLCAPNGATPSGAYWSSTTGNSNNISFPGGSPSAGPYTFNYADVGRVELFMKRSAS
                     SSAEGSSGSFVVRPHGFVLNNIRCTTANADSCGGGALPTGLNPGAIAAGGPTFIRAGH
                     PFSVTVTARNAAGAATPNYGRESPPETVRLVPTVTGAGGPNSNPAIGGSFGSFNNGVA
                     TGTNFTWNEVGIIRLTPGVGDEDYLGAGDVVGTQSGTVGRFFPHHFSVSGTLTTRSQF
                     PQTVTTGSIAAGGTTLVVASAANIAPGHPLTVAGAGPAGADLNATVTRMSGTALTLAI
                     PAATAVSNAAVIKPLDPFTYMGEPMMLQLQVRAHSASGTVTQNYADTYARLHAATVET
                     AATWFATGCSGTAMCFGLGAVNGTTAALTPRLARAGGIPSSAWTAGVGSFQVAVLLSR
                     PTSTVADMSWGPYEDLRFGAAPRDTDGVSLPTQAQHLAHGMDMDADLNNTPERRELFS
                     TSSRLGRLRIASAHGSELLPLPIGLSAQYWNGTSFVTNHDDNNTRFGSSAVALSNFRG
                     QLDPGETSVASIPSSIVLAAGRGAYTLTKPSGGDGRYGGSVDMTVGGLAFLPGNVGRA
                     RFGIVKRANEVVHLRENF"
BASE COUNT         2869 a         5260 c         5312 g         3097 t
ORIGIN      
        1 cgagtagttc atgttggcga tgccgccttc ataaatcagg tcgacgatca gcttcagctc
       61 gttgaggcac tcgtagtagg cgatctccgg ctggtagccc gcctcgacga gcgtctcgaa
      121 gcccatcttg atcagctgcg tggcgccgcc gcagagcacg gcctgctcgc cgaagaggtc
      181 gctctcggtc tcctcggcga aggtcgtctc cagcagcccg acgcggccgc agccgatcgc
      241 ggtgccgtag gcgagcgcga ggtcgagggc ggcgccgctc gcgtcctggt ggacggcgac
      301 gaggccgggg gtgccggcgc ccgcctggta gagctcgcgc acgcggtgcc cgggcgcctt
      361 cggcgcgacc atgatcacgt cgaggtcggc gcgtggcacg acctggccgt agtggacgtt
      421 gaagccgtgc gcgaaggcca gcacggcgcc ctgcttggcg tgcggctcga cgttttcctt
      481 gtagacctgg gcgatgttct cgtccggcag caggatcatc acgacatcag cctgcttgac
      541 cgcatcgatg acttccatga cgttcaggcc ggcgttcttc gccttctccc aggatgcgcc
      601 tcccttgcgc agggcgacgg tcaccttgca gccggaatcg ttcaggttca gcgcgtgggc
      661 atggccttgc gagccatagc cgatgatggt gacgttcttg cccttgatga gggacaggtc
      721 gcagtctttg tcgtagaaaa ctttcatgat cttcctaact aggtaatggt tgattttgtt
      781 gatcagattt tgagaacgcg ttcgccgcgg ccgatgccgg agccgccggt gcggacggtt
      841 tccaggatcg cggtgcggtc gatcgattcg atgaaggcgt caagcttcga tttgttaccc
      901 gtcagttcga tcgtgtaagt cttctcggtg acgtcgatga tgcggccacg gaagatgtcc
      961 gcggtccgct tcatttcttc gcgctccttg ccgactgcac ggaccttgat cagcatgagc
     1021 tcgcgctcga tatgcgcgcc ttcggtcaga tcgaccacct tcacgacctc gatcagccgg
     1081 ttcaggtgct tggtgatctg ctcgatcacg tcgtccgagc cggaggtgac gatggtcatt
     1141 cgcgacagcg tcggatcctc ggtcggcgca acggtcagcg tctcgatgtt gtagccgcgg
     1201 gcagaaaaca ggccgaccac gcgcgacaag gcgcccgctt cgttttccag cagtacggag
     1261 atgatgtggc gcatgattac agatcctccg aaccgagcag catttcagtc aagcccttgc
     1321 ccgccttgac catcggccag acgttctcgg tttgatcggt aatgaagttc atgaacacca
     1381 ggcggtcctt catgccgaag gcctccttca gcgcgccttc cacgtcgccc ggcttctcga
     1441 tcttcatgcc gacatggccg aacgcttcgg ccagcttgtt gaaatccggc agggagtcca
     1501 tgtacgactc ggaatagcgc gagccgtaat cgatctgctg ccactggcgc accatcccga
     1561 ggaagcggtt gttcagcagg atgatcttgg gcgtcaggtg gtactgcttg caggtggcga
     1621 gttcctggat gcacatctgg atcgaggctt cgccggtgat gcatgcaaca gttgcatcgg
     1681 gattcgccat ctgcaccccc atcgcatacg gcaggcccac gcccatcgtg cccagaccgc
     1741 cggagttgat ccagcggcgc ggcttgtcga agtcgtaata ttgcgcggcc cacatctgat
     1801 gctggccgac gtcggaggtg atgaaggcgt cgcccttggt gacctgccag agtttctcga
     1861 ccacttcctg cggcttgatc acttcgttcg aggtcgaata ctttaggcat tcgcggccgc
     1921 gccattcatt gatctggcgc caccagcccg ccagcgcctg ggcattcggc ttcacctcgg
     1981 cggcgtcgag ctgcgacagc agttcgacca gcacgtcctt gacgttgccg acgatcggaa
     2041 tgtcgacctt cacccgcttc gaaatcgacg acggatcgat gtcgatgtgg atgatcttgc
     2101 gcggatgggt ggcgaagtgc ttcgggtttc cgatcacgcg atcgtcgaag cgcgcgccga
     2161 ttgcgatcag cacgtcgcag tgctgcatcg ccatgttcgc ttcataggta ccgtgcatgc
     2221 cgggcatgcc gacgaacttg tcgctggagg cgcgatatcc gcccagcccc atcaaggtat
     2281 tcgtgcacgg atagccgagc ttgtcgacca gcttgttcag ctcgggggcg gcgttggcca
     2341 ggatgatgcc gccgccggta tagatcatcg gccgctcggc cgacagcagc agctgcaccg
     2401 ccttgcggat ctggccggaa tggcccttgt ccaccggctt gtacgagcgc atgtcgactt
     2461 ccttcggata ctcgaacgcg cacttgtgca tcgtgatgtc cttcggaata tcgaccaaga
     2521 ccgggccggg gcggccggta gtggcgatga agaaggcttt cttcatcgtc gccgcaagct
     2581 ccttcacgtc cttcacgagg aagttgtgct tgacgcaggg gcgggtgatg ccgacggtgt
     2641 cgcactcctg gaatgcgtcc tggccgatcg cgtgcgaagg cacctggccg gagatcacca
     2701 ccatcgggat cgagtccatg taggcggtgg caaggccggt aactgcattg gtgacgccgg
     2761 ggccggaggt gacgatcgcc acgccgacct tgttcgagct gcgcgaatag gcgtccgcgg
     2821 catgaatggc ggcctgttca tggcgcacca ggatgtgctg gaatttttcc tgattgaaga
     2881 ttgcgtcgta gatgtagagc actgcgccgc cgggatagcc gaacacgtgt tcgacgccct
     2941 cctcggccag gcagcgcacg acaatttccg cgcctgtgag ttctgcactc attgcaagtc
     3001 ctttcaaggt ccattggaga ttgatcggat gctctcccct cgcgaatcac gcgcagactt
     3061 gcaggccagg gcttcccttc tttgtgcggt cacgccgctt cgttgcgcac ccgtagatgc
     3121 gtttcaaagc gccgcttaac gcgactcgtt cagccgaggt aactggaatg ccgctacgtg
     3181 tttctcacgc ttgtggcgtg ggttagagca attcggataa agcgcgtcta gctgggacag
     3241 gcgtcatgcg ccatgtacga gaagacagtc cttcagggtg cgaagggagt gataagttaa
     3301 tgcgtcgcac cattttggtc aagtttcctt gtgctgaatc cttttgaaat caagcggttt
     3361 tcggcgcttt ttgcgcgacg acaacccggg tttttggtag gatgcgcgca ccttcgccgg
     3421 gcgcagccgt tgccgctcat gcaagcttcc ggcgtgctgc aattacacgg atcagccttg
     3481 acaaccctcg cctcgcctat cccgatcccc gccccttcca tcaagcgccg cctggccagc
     3541 atgctttacg aggcgatgct gctgttcggc gtggtcgcct ttgccggcgc cgttttcgcc
     3601 gtcatgctcg aacagaagca cgcgctctac ctgcgccatc agcttcagta cgtgttgttc
     3661 ttcgtgctcg gactgtactt catctggttc tggacgcacg gcggccagac gctggcaatg
     3721 aagacctggc gcgtgcggct ggtcgatgcg agcggcaagg cggtgggcgc gggccgcgcg
     3781 ttgctgcgct acgtgctggc gtgggcatgg ttcctgccgg gactggcgct ggcggccgca
     3841 gtcggcgccg aaggttcgct catgctcctg attcccgcga cgaactttgc gctatgggcg
     3901 ctgacgccgt tccttgaccc gcaacgccag ttcctgcatg accggctggc cgggacccgg
     3961 ctggtggtgg tggaaccggc gccgcccagg aagagttaat ggatcgcgaa cgggtcctcc
     4021 acgccggcat agatccgttc catcaacttg cgctcgcgct caacaacggt cccgacgaag
     4081 cgctccggat gacgcatgcc cttgaaagcg tggtagccgc gctccaggaa ttcttgaagc
     4141 tgcatcaact gcccgagccg tgcgggcccg cgcatcacgc gcagggtcga gccgatcagc
     4201 ggtacgcgca ccaactggca cagagcccgc ccgacggaat ccatgtgggc gagctgcaag
     4261 cgtcgctcgt gggtggaggt cagcgagcgg aacaccgcga cgtagtcggc ttcgctgaaa
     4321 tccacgccga gcgcctcggc catgatgcgg tcgagctttt ccgacagggc gtccagcgcg
     4381 acggcttcgg cgatcgtgtg cagcgccgct tccggcaaca tgcgctccat cgtgggaacg
     4441 atgcgggcca ggttcgcatc ccgctcggtc aggtcatgcg agccgtacaa gtcggtcagg
     4501 aagaaacgag cggccggagc tgaatcgctt gcggccagga gatcggaatg cgtgcgggcg
     4561 atgcgatctg cctggaagcg gcgcaatgcg aggcggcgtg cgctgagcgc ggggtcggcc
     4621 gcagcggcct tgcgcgcacg cgtgacccgc tccagctccc tgcgcagctt gaatcctgtc
     4681 gtgtgtcggt ccatgggccg gttcctcctt gcaatccacg gcggatgcag acccgaagcg
     4741 aaaccttcat gccgggcctg cgtctgaatt gctttgcaag ccagcaaact gcgtaccagg
     4801 aagcacgaga cacccggagt tcgccacgcg gcacgccctg tccagcgcga gaccaactgt
     4861 ggaaaacgcg ctatgctcgg cccccttccc ctacagaccg gttttcgaat gatcgcctgg
     4921 ctgacccgcc tgctgctgat tctgcaagct gcaatcgcga cggcgctcta cctgctcttc
     4981 acccgcacgc tcggcatcca gaatgtgcta cttgccgccg caatggcgat tgccgccgta
     5041 cccgcggtac gcatactgat catcgccaac aatttcatgc tcgcgcatcg ctttcgcagc
     5101 ccgctgccgg agcattgcaa gctggactgg cgcggcaggc tcgccctttt cctcggcgaa
     5161 gtacgagctt cgctgacggc atcgtcatgg cacctgccgt tcaagcggtt tcacaagcgc
     5221 cccatcgccg gtacgcgcga gctgccggta ctgctggtgc acggctatgg ctgcaacagc
     5281 ggatactggg cgccgttcag cgtagccctc tctcgcgccg gcattaccca tcatgccctc
     5341 gacatggaac cggtgttcgg cagcatcgac gggtatgccg cgcatattca tgccgccgtc
     5401 gaagaactgt gtcgcgatag cgggcaagaa cgcatcgtga tcgtcgccca cagcatggga
     5461 gggcttgcgg tccgcgcgta cctgcgcgcg cacggaagca cgcgcgtggc gaaggtcatc
     5521 acactcggca cgccgcatcg cgggactgcc ctcgcgcggc tcggcgcggg aatcaattct
     5581 caggaaatgc tgtgggcggt ccgcgagcag gaaggcttgt gcagcaagtg gctgcgcgaa
     5641 ctcgcagcca gcgagaccga acagacttac cggttgtttg tctccattta ttcccatcac
     5701 gacaacatcg tgtcaccgca aacctcttcc cacctgccag gtgcccgaaa tatcgcgttg
     5761 cacggcatcg gccacgtggc gctggcgccg acccccaggg tgatggaact ggtgatcggc
     5821 gagatcaaga cgctggcggc ggacgaacag cgcgacccgt cgcgtcccgg gaccttgcgg
     5881 gacaaggtgc acgcggcagg gtgaggaccg tctcgcgctc gttctgcccg gcagcagtga
     5941 tatcctagcg ggcgagcaaa gcaagggaga ccgtcagcat gcaacagaaa gccccgcgcc
     6001 gcacgcgcga gcgcatcctc gaactgtcgc tgcgcctgtt caacgaattc ggcgaaccga
     6061 acatcaccac caccgtgatc gccgaggaac tgagtatctc tcccgggaat ctctactacc
     6121 acttccgcaa caaggacgac attgtcaact cgctgttctc gcagttcgaa accgaaatcg
     6181 gtcgcatgct gtcggtgccc gagggccgcc ggcccacgat ggaagatgtg tggctgtacc
     6241 tgcatctgag tttcgagctg gtctggcgct accgcttctt ctatcgtgac ctgagcgacc
     6301 tgctctcgcg caaccgcaaa ctggaactgc atttcaaaca gatcctttca cacaagacca
     6361 aggtggccag gcagctttgc ctgggcctgc gcgcggacgg ggcaatggat gcgagcgacg
     6421 aagagatcga ggcactctcg accaacatgg tggtggtcgc gacttactgg ctctcgtatg
     6481 aatacgtgcg caatcctcgc aagtacaccg agcagcaggc gatgtcggaa gcgctggtgc
     6541 gcggttgcta ccaggtcctt tcgctgatcg gcccctatct gcgcggcgaa acccatgtgc
     6601 tgttcgaaaa gctggcggcc gaatacctga cgaaactgag acaagcgccc tgagaaaccg
     6661 ggacagaaaa ttgatcgctt gccgtgccgg caaagccagt gtgagtcgtt cggcagcgcg
     6721 gcgcaaggcc gcaggcggca tttggtcagg gcttccggac acgctgcgtt gtatgatcgc
     6781 cgctttgccc gcccccctta catgaagaca ctctgcatct actgcggctc ccgcatggga
     6841 gcgtcccccg cctacgcgac tgccgcgcga cgcatggcgg ctgcgatggt cgccagcggc
     6901 gtttcactgg tctatggcgg cggcaaggtc gggctcatgg gcgtgatcgc cgatgaagtg
     6961 ctgcggttag gcggcgaagc cgtcggtgtc attcccgagg cgctgatggc gcgcgaagtc
     7021 ggccatacgg gcctgacccg gctgcacgtg gtcagggaca tgcacgagcg caaggccctg
     7081 atggcggaac tggcggacgg cttcgtggcg atgccgggag gcatcggcac attcgaagag
     7141 ctgttcgagg tgtacacctg ggcccagctc ggcctgcacg acaaaccgat tgctctatac
     7201 aacgccgccg gctactacga cggcctgatc gccttcctcg agcacgtcgt ccagcaaggt
     7261 ttcctcaacc gcgaccaggc ggcgcttctg ttgcacggcg aggatgccgc ggacctggtg
     7321 cagcgcctgc gcgccttcgt cgcgccgcct catgagaagg cgcttccatc cgaccaggcg
     7381 cgcagaatca tttaagctgg gccagcctgg tctccacgaa ggcgaccagg tccggcgaaa
     7441 gcgactggct ggcctttgcc cgtgcaaacg cctccatcgc ctcgcccctg cgctgctccg
     7501 cctgcagcga gatcccgaga cccatccacc agacgccgtt ctgcggggtg cgctgcagtg
     7561 cctgcgcgta ctgctcgatg gcttcggcgt gacgctcggc acgctgcagc aaggcggcga
     7621 ggaaggcgat gtagtcggcg cgctccgtgg catgctgtcg gctgcgctgc agcgtgtcga
     7681 tggccggctt caactcgccc ttctccacct gcagtcgcgc aagaatcatc gcgaacccgg
     7741 tctgccgtgc gtcctgctcc agcccttccc gggcaaggcg gaccgcctcg tccacgttct
     7801 tcccttccag cagaagcgcg atcagggcct gcctggctgc cgcgtgcccg gcatcgagga
     7861 tcagcgcctg ttcgaagccg ttttccgcgt ccgcgcgcct tccgtgctgc aatgcgacgg
     7921 tcgctttgcg aaactcgttc tcggcgcgct gggcacgggt cggctcctga acctgcttgg
     7981 tgatcggttg cgcggggacc cggctgtcca cgggagcagc gacgggctcg gcgcggtggg
     8041 cagccggcag tgcggcagct gggcgcaacg gtgatggcgg tgcggcgggt gagggaggga
     8101 acgccgccgg tgccgcgggg gccgacggca cccgtgcctg agccgccgcg tcagccgccg
     8161 tcatggccgc aagcgtgggc gccggctcgg cctccgcaga aggcggaagc accgctgcag
     8221 gcctctcttc cgaagcgacc ggcagagtag cgggtgcgga agcgaccggc agagtagcgg
     8281 gtgcggaagc gaccggctcg tctggccggg ccggcgctac cggattgaga ccgacatcca
     8341 tcttaagcgg cagctgggct tgcacgagat atggctgcgc aaccggccgc aggatgaacc
     8401 atgcgaccac tgcccccagc ccgaggaagg cgcccagcgc gacccaccac gccgggtgcg
     8461 gaccgcggcg ttgtggaaca gcgcgtacat gaccgccgaa cgggcttgcg ccgccgggtt
     8521 cggagcggcg cgcatccagc tccttcagca tctggttgat cagactcatt tcagcaccat
     8581 ccatccgatt ccactgaggc cgaccagcac cacggcactc gcagcccacg cccatggcca
     8641 ccacgctcgg cgggcggctc cggtgtcgcg cgccgcggcc ttcacgtgcc gtgccgccac
     8701 ctcttgcttg ccttcgccga agcagagcat caaggacttg tgcgcgagaa cattgatcag
     8761 gcgcggcgtg ccaccgcttg cagtgtgcag cgcgcggatg gcactgcccg aaaacaggcg
     8821 cccgccggag tagccggcca cggtgagccg atgcgccaga tacgaggcca tgtcgtccct
     8881 gccgagcgat ccgagatggt aatggaaggt gatccgctgg ttcagctggc ggatggcttc
     8941 ctgctgcagc ttgcgatcga gttccggctg tccgaacatc acgatctgga gcagcttgcg
     9001 cttttcagtc tccaggttgg tcagcagccg cagtgcctcc agggattcga tcggcatggc
     9061 ctgcgcctcg tccaggcaca gcaccggacg cctgccctcg cgcgccagga tcagcagccg
     9121 atagttgatc gccttgagca gctgatgctg atcggcctcc ggcgccaccg acacttcgag
     9181 ttcgtcggcg agcgcgagca tcagcgcgcg cggctccagg tacgggttgg gcacgtaggc
     9241 ggtgacgaag cccttgtcca gcgaggccat gaacttgcgg caaagcaggg tcttgccggt
     9301 cccgacctcg ccggtgatct tgatgaagcc ctcgccgttc ttcaccgcca ccagcagcgt
     9361 gttcagcgcc tcctggtagg tggtgcaggc aaagaagaag ctggtgtccg gcgtgatgcc
     9421 gaatggcagc tcgcgcaggc cgaagtaggt cttgtacatc gcctaccagc gcagcggctc
     9481 ttcccgcggc gccagacgct ggatgcgttg ctggctgtcg agaatgtcct gcgcccagcc
     9541 ggtgtcgtcc tgcacgatgg tgggtttgag caggatgacg agctcgcgct tctggctcac
     9601 ctgattggtg ttgccgaaca aggcaccgat acccggcgcg ctgcccaccc ccgggatctg
     9661 cgagcgatcg ttacggctcg cgtgccgcat cagtccgccg atcgccacga tctggccgtc
     9721 gcggccgcgc acgatgctgt cggtctcgga agtgttgctc gcagcaagcg gcaggcgcag
     9781 cgaaccgccg gtacccagat cgatgctctt ggtgaccgtg tcgacctggc tgaccgaggg
     9841 acgcacgtgc aggatgatga agccgcgctc gtcgatctgg ggcgtcacgt cgagcacgac
     9901 tcccgagaag aacggctgca gggtgaccga aggcgtggtg acggtggcat tgccggtggt
     9961 tgtcgtcgta ctgacgttgg tgacgaagaa ctcgtcggtg ccgatcttca gcaccgcttt
    10021 ctgattgttc agcgtcgcaa tgcgcgggct tgaaagcaca tgcacattgc cctgcgattc
    10081 aaggaaggaa atgagcgccg agaaattgct ggtctggaat gccaggccaa acagcgaacc
    10141 ggtcgcggaa gtgagtgcgc cgatcgcgct gcccggcgtg gcggccacgc cggtggaacc
    10201 cgggcttgca accgtcgccg gcacattgcc cgctcccaat ggagccagcg cggtaccggg
    10261 agacagcagg ccgaacgagg cgggactgtt ggcggaggtg cgaaaagcgg cccagttgat
    10321 gccggtttga taggcctcgt tgagctgcac ctcgaggatc ttggcttcga ggatgacctg
    10381 gcgcgcgacc gatgcctggg tcgccttcag aaaggcgccg acattgcgca gctcatccgg
    10441 catgccgcgc acgaccacca cgccagactg cgggctgatg acgacgctgc gcccttctct
    10501 gccgacgccg acaatcgcct cgagcgccag gcgcagttcg gaccagaaat cgctgtcgga
    10561 ctcggtgctc accttgctgc tgtcgcgggc gatggcggtc gtctgcccgg gggcctgcgc
    10621 aggctggcca agctgattga cgattccggt tgcggtgttg gcagtcccgg catcggcgac
    10681 cgaggtggaa gtcacgcgca attcggaggt gccgcggcga ttgcccgtca ggtagttgac
    10741 ctggaagacc tgcgtctgca atgaagcggg acggaccgtg atgcggttgc cgtcgacacg
    10801 gtagtcgaaa ccatgaagct cacggatggc gtcgagcgcc tcgagcacgg tcacgtcgcg
    10861 cagattggcg gaaatcgtgc cggtgacgtc cgggtgcacc agcatgttgt agcgggtgcc
    10921 ggagacgatc gccatgaaga actggtcggc cggtacgttg ttgaacgtga cgttgaagcg
    10981 ctcttcggcc ggctgggtcg gcttcgggat ctcaatctgc aacggaggaa gcagcgccgc
    11041 actcaccgca tccggcgccg ctggctgctg ggcaagctgc gcagcgttgc caagctccgc
    11101 gctgatcatg tcgtaggtgt cccgcttggc cggtgctgcg caggcggcga gcgaggccgc
    11161 gaccaagatt attgtggctt tcctcaccat cgtcccttgc attattgctg cttgttcttg
    11221 ttggccggca gcgcctgcgc cgcgaccggg gatttcttct ccacctccgg atacatgcgg
    11281 aggatgcgtg tctcgcttcc ttctcgcaaa accacttccg tctcctggat cctgagcacc
    11341 cgcgcattgc ccaccgcgtc cccaaccgcg acggtacgcc cgccaatgac ggccaggcgc
    11401 cgctgcggcg agatcaggat ggactgaagc gcaagcgcgt cggcaaccgg tgctgcgacg
    11461 gcgttttcga cgcgcccctg gcccaaggcg gatggcaagc gggtggggtc cggcaaggac
    11521 tggcaaagac cgaccaccgg catggaacag gctgcggcca ggactgcctg accaaaacgg
    11581 atcagatgtg cagccatcgc ttgtccaggc tcaaggtgta caccgtcacc gtcatcgagg
    11641 cgttcgggtg attctcgact gtgaatgcaa gatcccccca gaacagctgc gtggaaaggc
    11701 tttccagctc cgtcagatag ttcagcatgt cgagatagcc tccctccacg gtaagctcga
    11761 caccatggcg atacaggccg gtctcccgga ggcctgcgtc caccatctct tcgccatgtg
    11821 tccgcgccag gttcaccaca ggaagctttt tcaggctcac gaggcgaagc cgagtgttgc
    11881 gagaaaggat gttctccaga agcggggcaa ttctatcagg agagaccagc cctctttgca
    11941 ttccggcaag cgattcctgc aggcccgctt tctgttgctg cagcagttgc aggcgctgtc
    12001 ttgcatcggc atcgggatcg gctgtgctct cggccacctt gcgcgcgatg tccgcccgga
    12061 gctgcgcaat ctgcgcctgc tggtcctgca tgtgccgcgc gatccgcttc tgttcagcgt
    12121 ccttcgggtc cagcgccagc atgttcattg ccgtcacgag tgcaagcgcc gccatcagga
    12181 agatgatcac ccgctcgcgc agggtcagcg cgtcgacgcg cgcggagaat ttttcccagt
    12241 gccgcttcat tgcagctccc tgcccgccga tgccggcgcc ggtcgttcgc tggactgcag
    12301 gcggaactcg acgaaagccg gcgcttgcgc ggaagaacgc gctgcagcag ccggcgcacc
    12361 catttccagc atgtcgaagg acttgttgcg catgcttggc tccctgccga gccgattgag
    12421 gtaggcaggg agcagctccg gacgcaaggc gccgccggaa agaccgatct cgttgccacc
    12481 gtcccgaatc gagaagccgg tcagccacac accatcgacg atctgtctgg agaacgcgcg
    12541 catgtactcg gaatagccgc cgggctgacg gttgtcgccg cgagagagcg actgcgcaag
    12601 gcgctcaagc gcgatcagct ccgcctccac gcgccgaagg tcagcctgta gcaggccatc
    12661 cttctgacgc gtgccgtgcg cggcattgat ggcggcgagc tgggcttcag ccgcttccaa
    12721 ctgggccgtg acggcattcg cctcggcgcg catctgggcc aactgcacgc tggcataagc
    12781 caccatgaga cccgagccga gcaggatcag ccccaatgcc tgcagcatcg tggccgcgga
    12841 aaaatacttc ttctgcgtga ggaagattgg attaaataga ttgatctgct ggctcacagc
    12901 accttctcct cctggcgcag cccggcgccc accgcgagca gatactgccg ctgcacctcg
    12961 ggcgcgagga gcgcgggcac gcgctcaagg tccgtcacgc tctcgagcct gaaccgctcg
    13021 acgggaacgt aaaggttcgc ggaaagatgc tcctgcaggc cggcgtcgtc gtcaccgagc
    13081 gggccgatca gcagcttcga caagctcatg aactggaact gccgctcgac gtggtcaagc
    13141 gagcgctgca actccagtgt gacgcgttcc agcagcgctg ccttctgggt cgcatcggca
    13201 tggagcaatt gttcgaacgc gatgtcgatg cggcgcgaaa gacagagctc gcctgcgtga
    13261 ttgacggtca gcagaccgcc gtccggcccg aaggtcagca tggccaggcc ccgcccttcc
    13321 gtttccgcga gtgccgccag attgcgctgc gcgagctctg gaatatcgat gacgccaagc
    13381 ggtatcttcg cctgttcaaa cgcgcgctgg cgctgctgga tcacctggtt gcgtgcagcg
    13441 accgcataca tcgactggcc ccgcaccggc gcgttagtat ccgacggaat tgacagcaca
    13501 tcgatcgttg catcgtcgac gtggaaatcg agcatgtctt tcaaacgcca ccggatcgcg
    13561 atcttcaact cgtccaccgg aacattgggc gcttcgacag taatcaatcg atagtcgcca
    13621 gcacccagca tcgtcgtgca gcgaaagcgc tgcacgtgca actcttttgc gacacggtcg
    13681 agcaccgcgc cctcgctgcc cggttcgcac gggaaggacg cggcgagctg cacgcacgga
    13741 cgaccgccgg ccggtcggac cacatgcacg gccaggaccg ctcctctctc gataaggagc
    13801 gccagccagc cctgctcggt ttttgatttc gcgaagaaac gcattgcttt gtggcatctc
    13861 aagaatgccc cgaacttagg gtaataacca ctcggtgtca acggaattac gtgcacacgc
    13921 tgccggggcg cgacagagta tttacatacg gcaacttgct agaaattttc acgcagatga
    13981 acgacttcgt tggcgcgctt gacgataccg aagcgggccc gcccaacatt accgggcaga
    14041 aatgcaagcc ctcctacggt catgtccacg ctccccccgt agcgcccatc tccccctgat
    14101 ggtttggtaa gcgtatacgc gccgcggccg gcagcgagca cgatgctcga aggaatcgat
    14161 gcgacgctgg tctcgcccgg atcgagttgc ccccggaagt tggacagcgc cacggcactg
    14221 ctgccaaacc gggtgttgtt gtcgtcatga ttggtcacaa agctcgtgcc gttccagtac
    14281 tgcgcgctca ggccgatcgg aagcggcagc agctcggatc catgcgcgct cgcgatgcgc
    14341 agacgcccga gccggctgct ggtcgaaaac agttcacgcc gctcgggcgt gttgttcagg
    14401 tcggcgtcca tgtccatgcc gtgcgccaga tgctgggcct gcgtgggcag gctgacgccg
    14461 tcggtgtcgc gcggcgcggc gccgaagcgc aggtcttcgt atggacccca gctcatatcg
    14521 gcgaccgtgc tcgtcggccg tgacagcagc acggccacct gaaaggaccc gacacccgcc
    14581 gtccatgcgc tcgacgggat gcccccagca cgtgcaagcc ggggcgtgag cgcggcagtc
    14641 gttccgttca cggcaccgag gccgaagcac atcgccgtac cgctgcagcc ggttgcaaac
    14701 caggtcgcag cggtctcgac cgtcgccgca tgcaggcgag cataggtgtc tgcgtagttc
    14761 tgcgtgaccg tgccgctcgc actgtgcgcc cgaacctgca gctgcagcat catcggttca
    14821 cccatgtagg tgaacggatc gagcggtttg atgacggccg cgttcgatac cgcggttgcg
    14881 gcgggaatcg caagggtgag cgccgtgccc gacatgcgcg tcaccgtcgc gttcaagtcg
    14941 gcaccggcgg ggccggctcc cgccacggtg agcggatggc caggcgcgat gttcgctgcc
    15001 gaggcgacaa ccagcgtcgt tccgccggcg gcgatgctgc ccgtcgtgac ggtctgcgga
    15061 aattggctgc gcgtggtgag cgtgccgctc acgctgaagt ggtgcgggaa gaagcgtccg
    15121 accgtacccg actgggtgcc gacgacgtcc ccggcgccca gataatcctc gtcgccgacg
    15181 ccgggtgtga gacggatgat gccgacttcg ttccaggtga agttggttcc ggttgccacg
    15241 ccattgttga agctgccgaa gcttcctcca atcgcgggat tgctgttcgg gccgccggca
    15301 ccggttacgg tgggcacaag ccttacggtt tccggcggcg actcgcgccc ataattcggt
    15361 gtcgccgcgc ctgcggcgtt acgtgcggtg acagtgacgg agaatggatg accggcgcga
    15421 atgaacgtcg gacctcctgc tgcaatggcc ccgggattga gcccggtagg cagcgccccc
    15481 ccgccgcagc tgtcagcatt ggccgttgtg cagcgtatgt tgttgagcac gaagccatgc
    15541 ggccgcacca cgaacgaacc gctggagccc tctgcactgc tgctcgcgct gcgcttcatg
    15601 aaaagctcga cgcgtccgac atcggcgtag ttgaacgtgt acggacctgc cgacggtgag
    15661 ccgccgggaa agctgatgtt gttgctgttg ccggttgttg acgaccagta ggcaccgctc
    15721 ggcgttgccc cgttgggggc acaaagcggc aatgccgcgg acaccgccga gaacgcggcc
    15781 cgcacgccgg cattcgtcgc ggggttgtgg caggtgagcg cgaactgcat cgtttccgtg
    15841 cggacctgag acgcatgcag gcgggtcggc acgccggaat tattcaggtt ggtgatgtaa
    15901 atgccatcca gtgcctgcgc cgcggtctgc ggtccccagt tcatgatcgt gcactggccg
    15961 ggactgccga aggcaacgtt atgggagcac gggctgtcag taaatacata gcccggcacg
    16021 agcacggacg cggtatcgct ggcggagttg ttttcactca cgttgtcgaa gcgtgcgccc
    16081 gagaccgtgg cgacgttggt gacactggtc gacgcgctgg tcaccgatac ggtaagcgtc
    16141 agcgccggcg cataggtacc cggcgccagc gccgctcccg aataaacgca tgtgaccgtc
    16201 tgccccgatg cactgcagga ccaaccggcc ccgcccgccg acacgtaggc gagctgcggc
    16261 ggcaaggtat tggtgacggt tatcggcccg ggttcctcgt tcgggccgtt gtttccgacc
    16321 gtgatcaggt aggagccgtt cgttccgagt cgcaagctgt cgatgcgcgt catttcgatc
    16381 gcaagatccg cgaccggaac gttcggcatc gccacgatct cggccgagag cagcaccaga
    16441 tcctggccgc tgctgtatgt cgtggtcgca ctcgtttgcc cggcctgtat gaccggggcg
    16501 gtgaggtcgt agacatcgaa gtcaataccg tgagaggc
//
LOCUS       DATLGF010000311         6612 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_92193,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000311 DATLGF010000000
VERSION     DATLGF010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6612
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92193"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /gene="fabZ"
                     /locus_tag="VM406_09515"
     CDS             complement(<1..432)
                     /gene="fabZ"
                     /locus_tag="VM406_09515"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HVL76243.1"
                     /translation="MKTLDINQIKEYLPHRYPLLLVDRVLEWEAGKSITAIKNVTANE
                     EFFNGHFPHKPVMPGVLMIEALAQTAALLSFLTMGVKPDANSVVYFVGIDGARFKRPV
                     EPGDQLKMQVEILRTARGIWKYKAVGSVDGQVALEAELMCTM"
     gene            complement(429..1481)
                     /gene="lpxD"
                     /locus_tag="VM406_09520"
     CDS             complement(429..1481)
                     /gene="lpxD"
                     /locus_tag="VM406_09520"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HVL76244.1"
                     /translation="MGTRLRDLVERFGGQLIGDGEIEVSGIAPLDSADASHLAFLSNP
                     KLRAQVGRTRAAALIHAPADEAAVAASFGGARIVAANPYAYFARVAQLFASQAALVPP
                     PGVHPSACVDSAAQVAPTASVGPHVTIEAGAQLGENVRVDAGCYVGRDVRLGAGTHLH
                     ANVSVHAGCVIGARGIVYSGAVIGSDGFGFANDGGVWVKIPQTGRVVIGDDVEIGANT
                     TIDRGALADTVIEDGVKLDNQIQIGHNCHIGAHTAMAGCVGVAGSARIGKHCTFGGAA
                     MVLGHLTIADNVHVSSGSMVSRSIHEPGQYTGFYPLAKNSDWEKSAAIVRNLSALREK
                     IRELEKTIKSLTGKQE"
     gene            complement(1500..1961)
                     /locus_tag="VM406_09525"
     CDS             complement(1500..1961)
                     /locus_tag="VM406_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HVL76245.1"
                     /translation="MLLPIAAAQAQEARIGFVNTERIFREAAPAKAAQAKIDQEFSRR
                     DKELQDLANRIKGMADKLDKDAPVLSETDRMRRQRELADMDKDFQRRQREFREDLNHR
                     RNEELAGVLDRTNRVIKQIAEAEKYDIIFQEAVYASPRIDITDKVLRALNK"
     gene            complement(2055..4409)
                     /gene="bamA"
                     /locus_tag="VM406_09530"
     CDS             complement(2055..4409)
                     /gene="bamA"
                     /locus_tag="VM406_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HVL76246.1"
                     /translation="MKLITKRFALPLFPRRLISSAVLALCAGHAFAVDPFTVRDIRVE
                     GIQRTEAGTVFSYLPLRVGDTYTDERGAAAIKALFGTGFFKDVRLEVEGDVLVVVVEE
                     RPAIAAIDFSGVREFEREQLNKAMRDIGLAEARIFDRALVERAEQELKRQYLARGLYG
                     VEVTTTVTPVERNRVNISFAVDEGDVARIRQINIIGNKAFSDSALRSVLSLRTPGWFT
                     WYSKADQYSRQKLAGDIEALRSYYLNRGYLEMQVESTQVSISPDKRDIYITININEGQ
                     RYTVAGINLEGEMLGQEEEFRSLLQLRPGDVYSGEKLAESVRRISERMGNFGYAFANV
                     NASPEIDREKGQVNFTVLIDPGQRVYVRHINIEGNTRTRDEVVRREFRQFESSWYDGE
                     KIRLSRDRVDRLGYFTEVQIETPEVPGTTDQVDVNMRVAEKPTGNIMIGAGFSSSEKL
                     TLSGSIHQANAFGTGNTLGVDVNTSKINRTFAISTTQPYFTDDGVSRSYEVFLRTQRP
                     PEVNTGDYVIETAGANIRFGVPFSEVDTVFFGAGVERNNVRTYFGQPGFNDSPELYRI
                     HVSRYGAAPTPETPGVFDASTTSFPLTVAWQRDSRDSALIPTSGRYQRANLELAPAGS
                     LRYYRAVYQHQYFYPMFRNATLALNGEVGYGRGVGGSYPVFKHFYAGGIGSVRGYRGA
                     SLGPLAANGDPLGGASRIVGNVELQFPFPGADRTLRWFTYLDAGNVYAEGVTPSLRDL
                     RYSAGVGISWISPIGPLKLSFGRPLNAKAGDRKESFQFQIGTGF"
     gene            complement(4460..5815)
                     /gene="rseP"
                     /locus_tag="VM406_09535"
     CDS             complement(4460..5815)
                     /gene="rseP"
                     /locus_tag="VM406_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HVL76247.1"
                     /translation="MTFLHTVLAFLAALAVLIVVHELGHYWVARLCNVKVLRFSVGMG
                     RVVWSRRFGPDQTEWALSALPFGGYVKMLDAREQDVGTLSEQDLKREFTRQSVWKRIA
                     IVAAGPLANFLLAIALFAGLYSYGLPEPAATLRDVPQQTAAYEAGLRGGERVRAVDGS
                     AVQTWGELRWKIMQAVLDQRPARLEVEDGEGRGAVRQVTLRLGGVSASDLETDFLGRL
                     GLDLARPPAVLGRIVPGGPADAADLREGDRVLSVNGEPVADGLAFVERVRTAPGQELA
                     LQVQRAGQQLQRVVRPEAHSVRDQVVGRIQVEVPLAPDMVTVRSGPLEAVARGAERTW
                     DTSVLTLKMLGKMLVGEISWKNISGPITIADYAGQTARIGLVSYLTFLAFISISLGVM
                     NLLPIPVLDGGHLLYYSLELLTGRPVSERFGEIAQRAGVGILMVLMAVAVFNDILRLV
                     S"
     gene            complement(5812..>6612)
                     /gene="dxr"
                     /locus_tag="VM406_09540"
     CDS             complement(5812..>6612)
                     /gene="dxr"
                     /locus_tag="VM406_09540"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HVL76248.1"
                     /translation="ALVMSGSLFMEAVHASGADLLPIDSEHNAIFQCLPAGYQRSPAR
                     HGVAKILLTASGGPFLNRPVETLERVTPAEAVAHPNWVMGRKISVDSATMMNKGLEVI
                     EAHWLFGAAPDQIEVVIHPQSVVHSMVSYIDGSVLAQMGNPDMRTPIAHALAYPDRVE
                     SGVGAVDLARIGQLQFFAPDFERFPCLRLAYDALFAGGSAPAILNAANEIAVEAFLSG
                     SIGFRAIDRLIARVMERVPTAALTSIEALLDHDRRARECARALLTEVT"
BASE COUNT         1199 a         2167 c         2078 g         1168 t
ORIGIN      
        1 catcgtgcac atcagctcgg cttcgagggc gacctgcccg tccaccgagc ccaccgcctt
       61 gtacttccag atgccgcgcg ccgtgcgcag gatttccact tgcatcttca gttgatcgcc
      121 cggctcgacc ggacgcttga agcgcgcgcc gtcgatgccc acgaagtaga cgacgctgtt
      181 cgcatccggt ttcaccccca tcgtcaggaa agacagcagc gccgccgtct gcgcgagggc
      241 ctcgatcatc aggacaccag gcatgaccgg cttgtgcggg aagtggccgt tgaaaaactc
      301 ctcgttcgcc gtcacgttct tgatcgccgt gatcgacttg ccggcctccc actccagcac
      361 ccggtccacc agcagcagcg ggtagcgatg gggcaggtat tccttgatct gattgatgtc
      421 gagcgtcttc attcttgctt tccggtgagc gatttgattg ttttctccag ttcgcggatc
      481 ttttcgcgca gcgcagaaag attgcggacg atggcagccg atttttccca atcggaattc
      541 ttggcgagcg gatagaaacc cgtgtactga ccgggttcat ggatcgagcg cgacaccatg
      601 ctgccggagg agacgtgcac gttgtccgcg atcgtcaggt gcccgagcac catggccgcg
      661 ccgccgaacg tgcagtgctt gccgatgcgc gcggaccccg cgacgcccac gcatccggcc
      721 atcgcggtgt gcgcgccgat gtggcagttg tggccgatct ggatctggtt gtcgagcttg
      781 acaccgtcct cgatgacggt gtcggcaagt gcaccgcggt caatcgtcgt gttcgcgccg
      841 atctcgacgt cgtccccgat gacgacgcgc ccggtctgcg ggatcttcac ccacacgccg
      901 ccatcgttgg cgaagccgaa gccgtccgag ccgatcacgg cgcctgaata cacaattccg
      961 cgcgcgccga tcacgcagcc ggcatgcacg ctcacgttcg cgtgcaggtg cgtgccggcg
     1021 cccaggcgca cgtcgcgccc gacatagcag ccggcatcca cgcgcacgtt ttcgccgagc
     1081 tgcgcacccg cttcgatcgt gacatgcggt ccgactgacg ccgttggcgc gacctgggcg
     1141 gcggaatcga cgcaggccga cggatggacg cctggcggcg gcacaagcgc agcctggctg
     1201 gcaaacagtt gcgcgacgcg ggcgaagtat gcgtacgggt tggcggcgac gatacgcgca
     1261 ccgccgaacg aggcggcgac agccgcctcg tccgcaggtg catggatcag ggccgcggcg
     1321 cgggtgcgcc cgacctgcgc gcgaagcttc gggttggaga gaaacgcgag atgggaggca
     1381 tcggcgctgt cgagcggcgc gatgccggac acctcgattt ccccatcccc gatcagctgg
     1441 cctccgaagc gttcgaccaa gtctcttagt cgagtgccca ttccggcctc atgaggtaat
     1501 tacttgttga gcgcgcgcag caccttgtcg gtgatgtcga tgcgcgggct ggcgtacacc
     1561 gcttcctgga agatgatgtc gtacttctcc gcctcggcga tctgcttgat cacgcggttg
     1621 gtgcggtcga gcacgccggc cagctcttca ttgcggcggt gattcaggtc ctcgcggaac
     1681 tcgcgctgcc gacgctggaa gtccttgtcc atgtcggcca gttcgcgctg acggcgcatg
     1741 cggtcggttt ccgacagcac gggcgcgtcc ttgtcgagct tgtcggccat tcccttgata
     1801 cggttggcca ggtcctggag ctccttgtcg cggcgcgaga attcctggtc gatcttggcc
     1861 tgggccgcct tggccggcgc cgcttcgcgg aagatgcgtt ccgtattcac gaaaccgatc
     1921 cgcgcctcct gcgcctgggc cgccgcaatc ggcagcagca tggcggcggc gaggaacaaa
     1981 cccttcagcg gcttggtaaa gctaattgca gacatcttgt tctccgaata tcaaattgcc
     2041 ggaataacgc ggcatcagaa gccggtgcca atctggaact ggaagctttc cttacggtca
     2101 cctgccttcg cgttcaaggg ccggccgaag ctcaatttca gcggaccgat cggcgaaatc
     2161 cagctgatgc cgacgccggc agagtagcgc aggtcgcgca gggacggcgt gacgccctcg
     2221 gcatagacgt taccggcgtc aaggtaagtg aaccagcgca gcgtgcgatc ggctccgggg
     2281 aacggaaact gcagttccac gttcccgacg atgcgcgagg caccgcccag cgggtcgccg
     2341 tttgccgcca acggcccgag cgatgccccg cgataaccgc ggacggagcc gatgccgccg
     2401 gcataaaagt gcttgaacac gggatagctc ccgccgacgc cgcggccata gccgacttct
     2461 ccattcaagg ccagcgtggc gttgcggaac atcggataga agtattggtg ctggtagacc
     2521 gcacggtagt acctcagcga accggcgggc gccagctcca gattggcgcg ctgataacgg
     2581 ccgctcgtcg ggatcagcgc gctgtcgcgg ctgtcgcgct gccatgccac cgtgagtggg
     2641 aagctcgtgg tactggcatc gaagacgcct ggcgtctccg gcgtcggcgc cgccccgtag
     2701 cgcgagacat ggatacgata caactccggg ctgtcgttga aaccgggctg gccaaagtat
     2761 gtgcgcacgt tgttgcgttc gacgccagcg ccgaagaaca ccgtgtcgac ctcggagaac
     2821 ggaacgccga agcgaatgtt cgcgccggcg gtttcgatca cgtagtcacc ggtattgact
     2881 tcggggggac gctgcgtgcg caggaagact tcgtagctgc ggctgacccc gtcgtcggtg
     2941 aaatacggtt gcgtggtgga gatcgcgaac gtgcggttga tcttgctcgt gttgacgtct
     3001 acgccaagcg tattgccggt accgaaagcg ttggcctgat ggatcgagcc cgacaaggtc
     3061 agcttttcgc tgctcgagaa accagcgccg atcatgatgt tgccggtcgg cttctcggcc
     3121 acccgcatat tgacgtcgac ctggtcggtc gtgcccggca cttccggcgt ctcgatctgc
     3181 acctcggtga agtaaccaag ccggtcgacg cggtcgcgcg acaggcggat cttttcaccg
     3241 tcgtaccagg agctctcgaa ctggcggaac tcgcgccgca ccacttcgtc gcgggtgcgc
     3301 gtgttgcctt caatattgat gtggcgcacg tagacccgct gaccgggatc gatgagcacg
     3361 gtgaaattga cctgcccctt ctcgcgatcg atctccgggc tcgcgttcac gttcgcgaat
     3421 gcgtagccga aattgcccat gcgctccgag atccggcgca cgctctctgc aagcttctcg
     3481 cccgaataca cgtcgcccgg ccgcaattgc aacagcgagc ggaactcttc ctcctggccg
     3541 agcatctcgc cttccaggtt gatgccggcc accgtgtagc gctgtccttc gttgatgttg
     3601 atggtgatgt agatgtcgcg cttgtccggg ctgatggaca cctgcgtcga ctccacctgc
     3661 atttcgagat aaccccgatt caggtagtac gagcgcagcg cctcgatgtc gccggccagt
     3721 ttctggcgcg aatactggtc ggccttcgaa taccaggtaa accagcccgg ggtgcgcagc
     3781 gacagaacgc tgcgcagcgc ggaatcggag aacgccttgt tgccgatgat gttgatctgg
     3841 cggatgcgcg cgacatcgcc ttcgtccacg gcaaaactga tattgacgcg attgcgctcg
     3901 acaggcgtga cggtggtcgt cacctcgacg ccgtacagac cgcgcgccag gtactggcgc
     3961 ttgagttcct gctcggcgcg ctcgaccagc gcacggtcga aaatgcgcgc ctcggccagg
     4021 ccgatgtcgc gcatcgcctt gttcagttgt tcgcgctcga actcgcgcac gccggagaaa
     4081 tcgattgccg caattgccgg acgctcctcc accacgacga cgaggacatc gccctccact
     4141 tcgagacgca cgtccttgaa gaagccggtg ccgaacaggg ccttgatcgc ggccgcgcca
     4201 cgctcatcgg tataggtgtc gccgacgcgc agcggcagat agctgaacac cgtgccggcc
     4261 tcggtgcgct ggatgccctc gactcgaatg tcacgtacag tgaacggatc cacggcgaat
     4321 gcatgaccgg cgcaaagtgc gagaactgcg gaggaaatca ggcggcgggg aaacagcggc
     4381 agggcaaagc gcttggtaat caatttcatc gaatgtcttg ttatcaaact cccgccgtcc
     4441 tgaaggcggc cggctcctat caggacacca gtctgaggat gtcgttgaat accgcgaccg
     4501 ccatcaaaac catcaggatg cccacaccgg cgcgctgggc gatctcaccg aaccgctccg
     4561 ataccggacg cccggtcaaa agttccagcg aataatacag taaatgccca ccgtctagaa
     4621 ccggaatggg aagcaagttc attacgccca ggctgatgct gatgaacgcg aggaaggtca
     4681 ggtagctgac gagtccgatg cgcgcggtct ggccggcgta atccgcgatc gtgatggggc
     4741 cggagatgtt cttccaggaa atttcgccga cgagcatttt accaagcatc ttcagcgtca
     4801 gaacgctcgt atcccaggta cgctccgcac ctctggccac cgcctccagc ggcccgctgc
     4861 gcaccgtcac catatccggc gcaagcggga cctcgacctg gatgcggccg acgacctgat
     4921 cgcggacgga atgcgcttcc ggccgcacga cgcgctgcaa ctgctgcccc gcgcgctgca
     4981 cctgcagcgc gagctcctgc ccgggcgcgg tgcgcacgcg ctcgacgaag gccagtccgt
     5041 cggccaccgg ctcgccgttc acgctcagca cgcggtcccc ttcccgcagg tcggccgcat
     5101 cggccggacc accaggaaca atgcggccga gcacggctgg cggcctggcc agatccaggc
     5161 ccagccggcc gaggaaatcg gtctccagat ctgacgcgga gacaccgccg agccgcagcg
     5221 tcacctgccg caccgcgccc cgcccttctc catcctcgac ttcaaggcgg gccggacgct
     5281 ggtcgagcac cgcctgcatg atcttccagc gcaattcacc ccaggtctgc acggcgctgc
     5341 cgtcgaccgc ccgcacgcgc tccccgccgc gcaaccccgc ctcgtaggcc gccgtctgct
     5401 ggggcacatc gcgcaaggtc gctgccggtt cgggcagacc atagctgtaa agccccgcga
     5461 acaacgcgat ggcaagaagg aagttggcca gcggacctgc ggcgacaatc gcaatccgct
     5521 tccacacgga ctggcgcgtg aactcgcgct tcaagtcttg ctcggagagc gtcccgacat
     5581 cctgctcgcg cgcgtcgagc atcttgacgt acccgccgaa cggcagcgcc gacagcgccc
     5641 attcggtctg gtcggggccg aaacggcgcg accagacgac gcgccccatg cccacggaaa
     5701 aacgcaggac cttgacgttg cacagcctgg ccacccagta atgaccgagt tcgtgcacga
     5761 cgatcaggac ggccagggcc gccaggaagg caagtacggt atgcaggaaa gtcatgtcac
     5821 ttccgtcaga agagcgcgcg cgcattcgcg ggcacgccgg tcgtgatcga gcaaggcttc
     5881 gatgctggtc agcgcggccg tcggcacgcg ctccatcacg cgcgcgatca ggcggtcgat
     5941 cgcgcgaaag ccgatgctgc cggaaagaaa ggcctcgacg gcgatctcgt tggccgcgtt
     6001 cagtatagct ggggcgctcc cgccggcaaa caacgcatca taggccaggc gcaggcaagg
     6061 aaagcgctcg aaatccggcg cgaagaactg cagctggccg atgcgggcaa ggtcaaccgc
     6121 ccccacaccc gattcgaccc tgtccggata ggccagcgca tgcgcgatcg gcgtgcgcat
     6181 gtcgggattg cccatctggg caagcaccga gccgtcgatg taggagacca tcgaatgcac
     6241 gacgctttgc ggatggatca cgacttcgat ctggtccggc gctgcaccga acagccagtg
     6301 cgcctcgatg acttcgaggc ccttgttcat catggtcgct gaatcgaccg agatcttgcg
     6361 ccccatgacc cagttcgggt gcgcaacggc ctctgcgggc gtgacccgct ccagcgtctc
     6421 gacagggcgg ttcaggaacg ggccgccgga ggctgtcaac aggatcttcg cgacgccatg
     6481 gcgcgcgggg ctgcgctgat agccggccgg caggcactgg aagatcgcat tgtgctcgct
     6541 gtcgatcgga agcaggtccg cgccgctggc gtgtaccgcc tccatgaaca ggctgcccga
     6601 catcaccagt gc
//
LOCUS       DATLGF010000312         5083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_228894, whole genome shotgun sequence.
ACCESSION   DATLGF010000312 DATLGF010000000
VERSION     DATLGF010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228894"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..1048)
                     /locus_tag="VM406_09545"
     CDS             complement(272..1048)
                     /locus_tag="VM406_09545"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HVL76249.1"
                     /translation="MNYENILVEVQGKVGLIRLNRPKALNALNDQLMDELGHALLGFD
                     ADDNIGCIVITGSEKAFAAGADIGAMADYTFMDVYKGGYISRNWETIRSIRKPVIAAV
                     AGYALGGGCELAMMCDIIIAADTARFGQPEVKLGTIPGAGGTQRLPRAVSKAKAMDLC
                     LTARMMDAAEAERAGLVSRVVSADKLLEEAMGAADTIAAMSLPSVMMIKESINRAYET
                     TLAEGVQYERRLFHSTFATEDQKEGMKAFLEKRLPNFRNL"
     gene            complement(1102..2337)
                     /locus_tag="VM406_09550"
     CDS             complement(1102..2337)
                     /locus_tag="VM406_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HVL76250.1"
                     /translation="MSAKRLSLLASVMLVALAGCTTPPSAPPAPTMPAPAIPPAAPPA
                     PSPAPPPVAQPPVQTLQVTTFQSIPGWNEDRLREAWPAFLTSCEVLVRRPDWREPCMV
                     AREVKGADEAAVRLFFESFFVPHQVFNADGSDQGLVTGYYEPLLRGARKRGGRFQTPL
                     HKEPADLLTIDLGSVYPELRNMRLRGKVVGNKVVPYPSRGQMMQDGALAGHELLWVDD
                     PIEAFFLQVQGSGRVYLEDTKETVRIAYANQNGHPYRSIGRYLVDKGEMTLDQASAQS
                     IKAWYAANPARREELLSANPSYVFFREEKLSEPIPGPKGALGVPLTDQRSIAVDPQFV
                     PLGAPVFLSTTQPNSTVPLRRLMLAQDTGGAIKGAVRADYFWGFGAQAGERAGKMKQR
                     GAMWVLLPKGQGGPATAAR"
     gene            2352..2513
                     /locus_tag="VM406_09555"
     CDS             2352..2513
                     /locus_tag="VM406_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76251.1"
                     /translation="MWLLMVEAAVALFLLVFIVWWTASGRKPDRPAPRDRAKQTPADK
                     AQAPDEKKD"
     gene            complement(2514..2888)
                     /gene="apaG"
                     /locus_tag="VM406_09560"
     CDS             complement(2514..2888)
                     /gene="apaG"
                     /locus_tag="VM406_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HVL76252.1"
                     /translation="MAAYEFTVSVRTQYLAEQSDPERSSFVFAYTITIRNTGDVAAQL
                     ISRHWVIVDANNRREEVKGLGVVGHQPLLRPGEQFEYTSGTALATPQGSMRGSYFCVA
                     EDGERFEARIPEFLLSLPRTLH"
     gene            3010..3675
                     /gene="rpe"
                     /locus_tag="VM406_09565"
     CDS             3010..3675
                     /gene="rpe"
                     /locus_tag="VM406_09565"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HVL76253.1"
                     /translation="MPNYRIAPSILSADFARLGEEVRNVVAAGADMIHFDVMDNHYVP
                     NLTIGPMVCEAIRPHVQVPIDVHLMVKPVDRIIPDFAKAGANIITFHPEASEHIDRTL
                     QLIRDHGCKAGLVFNPATPLHHLDHVMDKLDIILLMSVNPGFGGQSFIPQTLKKIAEV
                     RQRIDASGREIMLEVDGGIKIDNIAEVARAGADTFVAGSAIFGKPDYKAVIDAMRAQL
                     AQA"
     gene            3672..4376
                     /locus_tag="VM406_09570"
     CDS             3672..4376
                     /locus_tag="VM406_09570"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HVL76254.1"
                     /translation="MTGAPSMELNDIRAAIVDLDGTMVDTAPDFLVAINRMREELGLG
                     PLDIETIKRFVGKGTENLMRRVLAEDYDESGVEQHYARALASYTTHYLAINGQYSRLY
                     PGVKEGLESLRAKGLRLACVTNKPIAFALPLLEKTGLAGFFEIVYGGDSLPRKKPDPM
                     PLLQVCTDFGVTPRQVVAIGDSSNDAQAARAAGCRVLTVPYGYNHGEPIQAVDSDGIV
                     PTLRDAADIIPVSIQA"
     gene            4546..>5083
                     /locus_tag="VM406_09575"
     CDS             4546..>5083
                     /locus_tag="VM406_09575"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880785.1"
                     /note="with component II, the glutamine amidotransferase,
                     catalyzes the formation of anthranilate from chorismate
                     and glutamine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HVL76255.1"
                     /translation="MTELEFQSLASQGYNRIPLIAEAFADLETPLTLYLKLAQPQNGG
                     KNTFLLESVVGGERFGRYSFIGLPAATLLRSHGGRTEVVRHGEVVESHEGNPLEFITE
                     FQSRYRAAVRPGLPRFCGGLAGYFGYDTVRHIEKRLARSTPKDDLGLPDIQLLLTEEL
                     AVIDNLSGKLYLIVYADPA"
BASE COUNT          920 a         1585 c         1570 g         1008 t
ORIGIN      
        1 gggctctctg ttttgcgtca agctttttgt ttagcttttt cgcctaaccc cttctttttg
       61 caaaactttt cagtttcgca agctgccgaa tcacgacatt cgtttttcag tagcaaagaa
      121 gcgagattat tacgcccttc ggcagcgccg tcaacatctt tttgacgact gcggcaactt
      181 cgatactccc ggctgccgct gctgctccgt cgtttccctc ccctgaagct gttgcttcga
      241 tgggtcgtat ttcgacggga cgcgcacttt atcaaaggtt gcggaagttt ggcaagcgct
      301 tttcgagaaa tgccttcatg ccttcctttt gatcctcggt ggcgaaggtg ctatgaaaca
      361 agcggcgctc atactgcacg ccctcggcga gcgtggtctc gtaggcgcgg ttgatggact
      421 ccttgatcat catgacgctg ggcaaggaca tcgcggcaat ggtgtcagcg gctcccatcg
      481 cttcttccag cagtttgtcg gccgaaacca cgcgggaaac caggccggct cgctccgcct
      541 cggcggcatc catcattctt gcggtcaggc aaagatccat cgccttggcc ttggagacgg
      601 cgcgcggcag gcgctgcgtt ccgccggcac ccggtatggt gcccagcttc acctccggct
      661 ggccaaatct ggcggtgtcg gcggcgatga tgatgtcgca catcatcgcg agctcgcacc
      721 cgccgcccaa ggcgtagccg gcaactgcgg cgattacggg cttgcggatg ctgcggatcg
      781 tttcccagtt tcggctgatg tagccgcctt tgtagacgtc catgaaggtg tagtcagcca
      841 tcgcgccaat gtcggcgccg gcggcgaaag ccttctcgct tccggtgatg acgatgcagc
      901 cgatattgtc gtcggcgtcg aatccgagca gtgcatggcc gagttcatcc atcaactggt
      961 cattcaaggc gttgagcgcc ttgggacggt tcagccggat cagccctacc ttgccttgca
     1021 cttccacgag tatgttttcg tagttcatct tctctccaac tctatatacg gggtgagcgc
     1081 attgtactgc ccgggcccaa gctaacgggc agcggtcgcg ggcccgcctt gcccttttgg
     1141 cagcaagacc cacatggctc cgcgttgctt catctttccc gcccgctcgc cggcctgcgc
     1201 accgaacccc cagaagtaat ccgcccggac tgcccccttg atcgcgccgc cggtgtcctg
     1261 ggcgagcatc agccgccgca gcggcaccgt actgttgggc tgcgtggtgg aaagaaatac
     1321 cggagcgcca aggggcacga actgcggatc gaccgcgatc gagcgttgat ccgtcagcgg
     1381 gacgccgagc gcgcccttgg gcccgggaat cggctcgctc agcttttcct ctctgaagaa
     1441 tacgtagctt ggattggcgc tgagcagttc ctcgcgccgg gccgggttgg cggcatacca
     1501 tgccttgatg ctttgggcgg aggcctggtc gagggtcatt tcccccttat ccaccagata
     1561 acgtccgatc gaccggtacg gatggccgtt ctggttcgcg taggcgatgc gtacggtttc
     1621 cttcgtgtcc tccagataga cgcggccgga gccctgcacc tgcaggaaaa aggcttcgat
     1681 cggatcatcg acccagagca gctcatgacc ggcaagcgca ccatcttgca tcatctgtcc
     1741 gcgcgacgga tacggcacca ccttgttgcc gactaccttt ccgcgcaggc gcatattgcg
     1801 cagctcgggg tagacggaac caagatcgat ggtgagcagg tccgcaggtt ccttgtgcaa
     1861 tggggtctgg aaacgtccgc cgcgcttgcg cgcgccgcgc agcagcggct cgtaatagcc
     1921 ggtcaccaag ccctgatcgc tgccgtccgc attgaagacc tgatggggga cgaagaacga
     1981 ttcgaagaac aggcgcaccg ctgcctcatc ggctcccttg acctcgcgcg caaccatgca
     2041 gggctcgcgc cagtccgggc ggcgcaccag cacctcgcaa gaggtcagga aagccggcca
     2101 tgcctcccgc agcctatcct cgttccatcc ggggatggac tggaatgtgg tcacctgcaa
     2161 tgtctggacc gggggctgcg cgaccggtgg tggtgcaggg gatggcgccg ggggcgcggc
     2221 cggcggaata gccggagccg gcatagtcgg cgccggtggt gccgacgggg gcgtggtaca
     2281 gcctgcgagg gcgaccagca tgacggatgc gagtaaactg aggcgttttg ctgacattga
     2341 gggcgaacgg gatgtggctg ttgatggttg aagcggcggt ggcattgttt ctgctggtgt
     2401 tcatcgtctg gtggacggca tccggacgca agccggaccg gccggcaccg cgtgatcgcg
     2461 ccaagcagac gccggcggac aaggcgcagg cgccggacga gaagaaagac tgatcagtgc
     2521 agggtcctgg gcagcgagag caagaattcc gggatgcgcg cctcgaaacg ctcgccatcc
     2581 tcggccacgc agaaatagct gccgcgcatg gaaccctggg gcgtcgcgag tgcggtgccg
     2641 ctggtgtatt cgaactgctc cccggggcgc agcagcggct ggtggccgac cacgccgagt
     2701 cccttcactt cttccctgcg gttgttggcg tcgacgatca cccagtgcct ggagatgagc
     2761 tgggcggcga cgtcgccagt gttgcggatg gtgatcgtat aggcaaatac gaagcttgac
     2821 cgctccgggt ccgattgctc ggccaggtac tgcgtgcgga cggatacggt gaattcgtag
     2881 gcagccatgc ttttccttta ttgtcgcggc cggttcgtgc aagcatcgat tgcacctcaa
     2941 atcgcgcgcg cccgcaagac ggcgcggcgg gcgggataaa atacgccctt ctttcgcatt
     3001 gaggctctta tgccgaacta tcgcatcgcc cccagcattc tctccgccga cttcgcccga
     3061 ctgggcgaag aggtgcgcaa cgtcgtcgcc gccggcgcgg acatgatcca tttcgacgta
     3121 atggacaatc attacgtgcc gaacctgacg atcggcccga tggtgtgcga ggcgatccgg
     3181 ccgcatgtcc aagtgccgat cgacgtgcac ctgatggtca agccggtgga tcgcatcatt
     3241 ccggatttcg ccaaggccgg cgcgaacatc atcacttttc atcccgaggc gtcggaacac
     3301 atcgaccgca cgttgcagct gatccgcgac catgggtgca aggccggcct ggtattcaat
     3361 ccggccacgc cgctgcacca cctcgatcac gtcatggaca aactggacat cattctgctg
     3421 atgtcggtga atcccggctt tggcggccag tcgttcatac cgcaaacctt gaagaagatc
     3481 gcggaggtac gccagcgcat cgatgcatcc gggcgcgaga tcatgctcga ggtggacggc
     3541 ggcatcaaga tcgacaacat cgccgaggtc gcccgtgcag gcgcggacac cttcgtggcc
     3601 ggatcggcaa tcttcggcaa gccagactac aaggcggtga tcgacgccat gcgcgcgcag
     3661 ttagcccagg catgaccggc gcgccgtcga tggagctcaa cgacatccgc gccgccatcg
     3721 tcgacctcga cggaaccatg gtggacacgg cgccggactt cctcgtcgcc atcaaccgga
     3781 tgcgcgagga actcgggctc ggtccgctgg atatcgagac gatcaagcgc ttcgtgggca
     3841 aaggcaccga gaacctgatg cggcgggttc tggcggaaga ctacgacgag agcggcgtcg
     3901 agcagcacta cgcgcgtgcg ctggcgtcgt acaccacgca ttacctcgcg atcaacgggc
     3961 agtattcacg cctctacccc ggcgtgaagg aaggactgga gtcgctgcgt gccaagggcc
     4021 tgcgccttgc ctgcgtgacc aacaagccga tcgcctttgc gctgccgctg ctggagaaga
     4081 ccgggttggc tggcttcttc gagattgtct acggcggcga ttctctgcca aggaagaagc
     4141 ccgacccgat gccgctgctg caggtctgta ccgatttcgg tgtgaccccg cggcaggtgg
     4201 tggcaatcgg cgactcgtcc aacgacgccc aagccgcgcg cgcagcggga tgccgagtgc
     4261 tgaccgtacc ctacggttac aaccatggcg aacctataca ggccgttgat tccgatggta
     4321 tagttccgac gctgcgcgat gcagcggaca tcattccggt atccatccaa gcataatgtt
     4381 tctcgtcgaa aaacgtaccg tcccggggca gcatgaggcc tggctgtggc gacgctggca
     4441 ttcctggtcc aactgacccg atgccgctgg ccgtccatgg ccggtaacgt tttttgacag
     4501 tcatcccgct attgcgcgac gcgacaatag cgcccggaga gaaacatgac cgaactcgaa
     4561 ttccagtcgc tggcctcgca gggctacaac cgtattccgc tgatcgctga agcttttgcc
     4621 gatctcgaaa ctccactcac cctctatctg aaactcgcac agccgcagaa tggcgggaag
     4681 aacaccttcc tgctggagtc tgtcgtcggc ggagagcgtt tcgggcgcta ttcgttcatc
     4741 ggcctgccgg cggccacgct gttgcgcagc catggcggcc gcacggaagt cgtccggcac
     4801 ggcgaagtgg tggagtcgca cgaaggcaac ccgctcgagt tcatcaccga gtttcagtcg
     4861 cgctaccgcg ccgccgtgcg ccccggtctt ccgcgtttct gcggcggcct ggccggctac
     4921 ttcggctatg acacggtccg tcatatcgag aagcgccttg cccgcagcac gccgaaggat
     4981 gatctcggcc tgccggatat ccagctgctg ctgaccgaag aactcgctgt catcgacaac
     5041 ctgtccggca agctttatct gatcgtgtat gccgaccctg cac
//
LOCUS       DATLGF010000313         5860 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_72496,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000313 DATLGF010000000
VERSION     DATLGF010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72496"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VM406_09580"
     CDS             <1..520
                     /locus_tag="VM406_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PUA domain-containing protein"
                     /protein_id="HVL76256.1"
                     /translation="ALEAMAGGAGTGIGRGGMLTKILAAKRAATSGAHTVIAWGREDA
                     VLTRLASGEAIGTQLLAQTAQLTARKQWMADHLQTAGRVVLDDGAVQKLRSEGKSLLP
                     IGVVEVAGEFGRGDVITCVDERGAAIARGMSNYSSSEARRIMRKPSTEIAAILGFVEE
                     PELIHRDNMVLL"
     gene            complement(554..1144)
                     /locus_tag="VM406_09585"
     CDS             complement(554..1144)
                     /locus_tag="VM406_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HVL76257.1"
                     /translation="MRISATRKPAAKAGLAHRPVPRLQAAIMLAALSVGSAAWAQSAQ
                     FEEDFDDPYKPWQEIAIQLPAPPQPENLLPFEVSGNATHRFAVDAQSVNVGTDGVVRY
                     TLVTMSSAGARNVSYEGLRCNTGEVKLYAFGHPDGSWSRSRRDQWQSIRGNQTNRHHA
                     VLAREFICQERTIAGNASQIVFRIRNGQSLAPALGD"
     gene            complement(1145..1726)
                     /locus_tag="VM406_09590"
     CDS             complement(1145..1726)
                     /locus_tag="VM406_09590"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HVL76258.1"
                     /translation="MLDREGFRPNVGIILLNTHNEVWWGKRVREHSWQFPQGGIKYGE
                     TPEQAMFRELEEEIGLRAEHVKIIGRTRDWLRYEVPDRFIKREVRGHYRGQKQIWFLL
                     RMVGRDCDINLRGTDHPEFDAWRWHDYWVPLDVVIEFKRDVYQKALQELSRFLTRVPQ
                     GQHRHGGRYLRQGHANRPSVETVAVTSKSRSES"
     gene            1916..3643
                     /locus_tag="VM406_09595"
     CDS             1916..3643
                     /locus_tag="VM406_09595"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HVL76259.1"
                     /translation="MRASRFFVSTLKEAPSDAEIVSHKLMMRAGMIKRLGSGIYTYMP
                     MGLRVIRKVENIVREEMNRAGAIELLMPVVQPAELWQETGRWDKMGPELMRVKDRHGR
                     DFAIQPTSEEVITDVVRNDIKSYRQLPINFYHIQTKFRDERRPRFGLMRGREFTMKDA
                     YSFDRDVAGMQQSYKIMYDAYVRIFERFGLQFRAVAADNGAIGGSGSHEFHVIADTGE
                     DALVYCPESDYAANIEAAEAVSLLPSRAAATEPLTKTPTPGKAKCEHVAELLGLPLAR
                     TVKSIVLAVEKEAPAETEIWLLLLRGDHDLNEVKAAKVAGLAQYRFATEAEIVDTFGT
                     PPGYLGPIGTKKPVKVVADRTVANMSDFVCGANEVDFHYTGVNWGRDLPEPLVADLRN
                     VVAGDPSPDGEGPLAIQRGIEVGHVFQLGTRYSEDMKATYLDEAGKPQLMQMGCYGIG
                     VTRILGAAIEQNYDERGIIWPAAIAPFEVVLCPMGYDRSAAVKEQADALYAALCAAGV
                     DVILDDRGERPGVMFADWELIGVPHRVVIGERGLKEGMLEYQGRRDAAATQVPVADMA
                     AFVSARLAG"
     gene            3670..4293
                     /locus_tag="VM406_09600"
     CDS             3670..4293
                     /locus_tag="VM406_09600"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HVL76260.1"
                     /translation="MRTWLLVAACMLAFAQGAQAGNQKEEALADGVRLALQRAISDAR
                     PPKPQFASIDDRIRYLHWLGEMSDRLARRMPDRETRIEFLETVWYEAKRAGLDPGLVL
                     GLIQVESAFRKYAVSPVGAHGYMQVMPFWTRVIGDGDRRRLFHMQTNLRYGCSILRHY
                     LDIERGNLYMALGRYNGSRGQAKYPNAVLAAWKQWEYTEPVILPVAR"
     gene            complement(4363..4860)
                     /locus_tag="VM406_09605"
     CDS             complement(4363..4860)
                     /locus_tag="VM406_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HVL76261.1"
                     /translation="MATAKKPAAKAAAKKPAAKKAAAKPAAKKAAPAKKAAPAKKAAA
                     KPVAKKAAAKPAAKKAAPAKKAAAKPVAKKAAAKPKTPRKPNAAFMKPMTPSETLGAV
                     IGANPLPRTEVTKKVWDYIKKNKLQDSANKRMINADDKLKAVFGGKKQVSMFEMTKLI
                     SNHLQ"
     gene            complement(5102..>5860)
                     /locus_tag="VM406_09610"
     CDS             complement(5102..>5860)
                     /locus_tag="VM406_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HVL76262.1"
                     /translation="AMMQEIAALHAAVKPIETLFVVDAMLGQDAINTAKAFNDALPLT
                     GVVLTKLDGDARGGAALSVRHVTGKPIKFTGVAEKLDGLEAFDPERMASRILGMGDIL
                     ALVQEAQKGVDIAAAKDLADKLKVGGKFDLNDFKAQISQMKKMGGLSGLLDKLPAQFQ
                     QAAGNTDMNQAEKQVRRMEGIINAMTPQERAKPELIKASRKRRIAAGAGVQVQEVNRM
                     LSQFEQMQSMMKKLKGGGMMKMMRGMKGMLPGLR"
BASE COUNT          958 a         1832 c         1927 g         1143 t
ORIGIN      
        1 cgcgctggag gcgatggccg gcggagccgg caccgggatc ggccgtggcg gcatgctcac
       61 caagatcctg gccgccaagc gcgcggcgac ctccggcgca cacacggtga tcgcctgggg
      121 gcgtgaagac gcggtgctga cccggctcgc cagcggcgag gcgatcggca cccagctgct
      181 cgctcagacg gcccagctga ccgcgcgcaa gcagtggatg gccgatcacc tgcagacggc
      241 cgggcgggtc gtgctcgacg atggtgcagt gcagaagctg cgttctgaag gcaagtcgct
      301 gttgccgatc ggcgtggtcg aggtggcggg ggagttcgga cgcggtgatg tcatcacctg
      361 cgtcgacgag cggggtgcgg cgattgcgcg cggcatgagc aattactcaa gctccgaggc
      421 gcgccgcatc atgcgtaagc cgtccaccga aattgcggcg attctcggct tcgtcgagga
      481 gccggagctg attcaccgcg acaacatggt actgctgtag tgcgtcaact ggcgtggcgc
      541 tgcccgggca cgctcagtcc ccgagtgcgg gcgcgagcga ttggccgttg cggatcctga
      601 agacaatctg gctggcgttg cccgcgatcg tgcgttcctg gcagataaat tcgcgcgcga
      661 gtaccgcgtg gtgcctgttg gtctggttgc cgcggatcga ttgccactga tcgcgccgcg
      721 aacgcgacca gctgccgtcg ggatgcccga acgcgtacag cttgacttcg ccggtgttgc
      781 agcgcagacc ctcgtagctc acgttcctgg cccccgcgct gctcatcgtc accagcgtat
      841 agcgcaccac gccgtcagtg ccgacgttca ccgactgcgc gtcgacggca aagcggtgcg
      901 tcgcgttgcc gctcacttcg aacggcagca ggttttccgg ctgaggcggg gcaggcagct
      961 ggatggcgat ctcctgccac ggcttgtagg ggtcgtcgaa gtcttcctcg aactgcgcgg
     1021 actgtgccca tgcggcactg ccgacggaca gcgcagccag catgattgcg gcctgcaggc
     1081 gcgggacggg gcgatgcgcc aagccggcct tggcagccgg cttgcgcgtg gcggaaatcc
     1141 tcattcagct ctcgcttctg gatttggaag tgacggcgac agtctcgacc gaggggcggt
     1201 tcgcatgccc ctggcgcagg tagcgcccgc catggcgatg ctggccctgc ggaacacgcg
     1261 tcaggaagcg cgagagttcc tgcaatgcct tctggtagac atcccgcttg aactcgatga
     1321 caacgtccag cggtacccag tagtcatgcc agcgccaggc gtcgaactcg ggatggtcgg
     1381 tgccgcgcag gttgatgtcg cagtcgcgcc ccaccatgcg cagcaggaac cagatctgtt
     1441 tctgcccccg gtaatggccg cgcacctcgc gcttgatgaa ccggtccggc acttcgtacc
     1501 gcagccagtc gcgcgttcgg ccgatgatct tgacgtgctc ggcgcgcagg ccgatctctt
     1561 cttccagctc gcgaaacatc gcttgttccg gcgtttcccc gtacttgatg ccgccttgcg
     1621 gaaattgcca cgaatgttcg cgtacgcgct ttccccacca cacctcgtta tgcgtgttga
     1681 ggaggatgat gccgacgttc gggcgaaagc cttcacggtc aagcatgttg cacctcgata
     1741 ctttctgcgg ctaaacgctc cctgccattg caattttcga acgcaacaag cgggccgctc
     1801 ggccacgctc gtattcgcca cgaattgttg gtttgtggaa tgggcgcatc agccagctaa
     1861 tcctttaaaa ttgaggtcga ttataacccc ctctttttaa aaagaattca ccgatatgcg
     1921 cgcctcgcga ttttttgttt ccacgctaaa agaagctcct tccgatgccg agatcgtcag
     1981 ccacaaactg atgatgcgcg ccgggatgat caagcgcctt ggttcgggca tctacaccta
     2041 catgccgatg gggctgcgcg tgatccgcaa ggtggaaaac attgtccggg aagagatgaa
     2101 ccgcgccggc gctatcgaac tgctgatgcc ggtcgtgcaa ccggccgagt tgtggcagga
     2161 aaccggacgc tgggacaaga tgggcccgga actgatgcgg gtgaaggaca ggcacgggcg
     2221 cgacttcgcg atccagccga cctcggagga agtcatcacc gacgtggtgc gcaacgacat
     2281 caagtcctac cgccagctgc cgatcaactt ctatcacatc cagaccaagt tccgcgacga
     2341 gcgccgcccg cgtttcgggc tgatgcgcgg ccgcgagttt acgatgaagg atgcctattc
     2401 cttcgaccgc gacgtcgccg gcatgcagca gtcgtacaag atcatgtatg acgcctatgt
     2461 gcgcatcttc gagcgcttcg ggctgcagtt ccgcgcggtc gcggccgaca acggcgccat
     2521 cggcggctcc ggttcgcatg agttccacgt gatcgccgac accggcgagg acgcgctggt
     2581 gtactgcccg gaatcggact acgcggccaa tatcgaagcg gccgaagccg tctcgctcct
     2641 gccttcacgg gccgctgcga ccgagccgct gacgaaaacg cccacgccgg gcaaggccaa
     2701 gtgcgaacac gtggccgaac tgctgggact gccgctggct cgtaccgtca agtccatcgt
     2761 gctggcggta gagaaggagg cgccggccga gacggaaatc tggctgctgc tgctgcgcgg
     2821 tgatcacgat ctcaacgagg tgaaggcggc gaaggtggcc ggcctggcgc agtaccgctt
     2881 tgcgactgaa gccgagatcg tcgacacctt cggcacgccg cccggctatc tcggacccat
     2941 cggcacgaag aagccagtca aggtggttgc cgaccggacg gtcgccaaca tgagcgactt
     3001 cgtctgcggc gccaacgagg tggacttcca ctacaccggc gtgaactggg gacgcgacct
     3061 gcccgagccg ctggtggcgg acctgcgcaa tgtcgtcgcc ggcgacccgt cgccggacgg
     3121 cgagggcccg ctggcgatcc agcgcgggat cgaggtcggg cacgtgttcc agctgggtac
     3181 ccgttattcc gaggacatga aggcgaccta cctcgacgag gccggcaagc cgcagttgat
     3241 gcagatgggc tgctacggca tcggcgtcac ccgcatcctc ggcgcggcga tcgaacagaa
     3301 ctatgacgag cgcggcatca tctggccggc cgcgatcgcg ccgttcgaag tcgtgctgtg
     3361 cccgatgggc tacgaccgca gcgcagccgt caaggagcag gcggatgcgc tgtatgccgc
     3421 gctgtgcgcc gccggcgtcg acgtcatact cgatgaccgc ggcgagcgtc ccggcgtgat
     3481 gttcgcggac tgggagctga tcggggtgcc gcatcgcgtg gtcatcggcg aacgcgggct
     3541 caaggaaggc atgctcgaat accagggtcg gcgcgacgct gcggcaaccc aggtgccggt
     3601 ggcggacatg gccgcattcg tgtcggcccg cctggccggc tgacgactcg cgccgctcgc
     3661 cacccgccaa tgcgcacctg gctgcttgtc gccgcatgca tgctggcgtt cgcccagggt
     3721 gcgcaggcgg gcaatcagaa ggaggaggcg ctcgccgacg gcgtgcggct ggcactgcag
     3781 cgcgcgatca gcgatgcccg tccgcccaag ccgcagttcg ccagcatcga cgaccgcatc
     3841 cgctacctgc actggctggg cgagatgtcc gaccgcctgg cgcggcgcat gccggaccgc
     3901 gagacccgga tcgaattcct ggagactgtc tggtatgaag ccaagcgcgc cggcctggat
     3961 ccggggctcg tgctcggcct gatccaggtc gaatcggcat tccgcaagta cgcggtctcc
     4021 ccggtcggcg ctcacggcta catgcaagtc atgccgttct ggacgcgggt gatcggcgac
     4081 ggcgatcgcc gcaggctgtt ccatatgcag acgaatctgc gctacggctg ctcgatcctg
     4141 cgccattacc tcgatatcga acgaggcaac ctttacatgg cgctggggcg ctacaacggc
     4201 agccgagggc aggcgaagta cccgaatgcc gtgctcgccg cctggaagca gtgggaatac
     4261 accgagccgg tgatcctgcc cgtggcacgg tgagcccggc agctcggaaa acaaaaacgc
     4321 ccgcgcgatg cgggcgtttt tgtttggcct ctggaaagac agttattgca ggtggttgga
     4381 aatgagcttg gtcatttcga acatcgatac ctgcttcttc ccgccaaaca cggctttcag
     4441 cttgtcgtct gcgttgatca tgcgcttgtt cgcagagtct tgcagcttgt tcttcttgat
     4501 gtagtcccac accttcttgg tgacttccgt acgcggcagc gggtttgcgc cgatcactgc
     4561 acccagggtc tcggaagggg tcatcggctt catgaaagcg gcgttcggct tgcgcggcgt
     4621 cttcggcttg gctgcggcct tcttggctac cggcttggct gctgccttct tcgcgggagc
     4681 ggccttcttc gctgccggct tggctgctgc cttctttgct acaggcttgg ctgctgcctt
     4741 tttggcggga gcggcctttt tggcgggagc tgcttttttt gctgcgggtt ttgctgcggc
     4801 cttcttggca gcaggcttct tggctgcggc tttcgccgcg ggttttttgg cggttgccat
     4861 cttaaagcct ccttcacagg aatgtgttgg gaatttgcgt caaatcacgc accgctaata
     4921 gtggtgcgct tttcagagtg ttgcaagttt tttttgattg tttggccacg ttggcgtata
     4981 cggggcgcga cgtggctgtt gcgcgtggat cgcgtggcgt gacgcggggc gtgcagaagg
     5041 gcgaatgacg cgaatgcggg gagggcgcgg ccggcacccg tggccggccg cggcaggggg
     5101 ttcagcgcag gccgggcagc atgcctttca tgccgcgcat catcttcatc atgccgccgc
     5161 ccttgagctt cttcatcatc gattgcatct gctcgaactg ggacagcatg cggttgactt
     5221 cctgcacctg cacgccggcg ccggccgcga tgcggcgctt gcgcgaggcc ttgatcagct
     5281 ccggcttggc gcgttcctgg ggcgtcatcg cattgatgat gccttccatg cggcgcacct
     5341 gcttttccgc ctggttcatg tcggtattgc cggccgcctg ctggaactgg gcgggcagct
     5401 tgtcgagcag gccggacagc ccgcccatct tcttcatttg cgaaatctgg gccttgaagt
     5461 cgttcaggtc gaacttgccg ccaaccttga gcttgtccgc caggtccttg gcggctgcga
     5521 tgtcgacacc cttctgcgct tcctgcacca gcgcaaggat gtcgcccatg ccgaggatgc
     5581 ggctggccat gcgctccgga tcgaaggctt cgaggccgtc gagcttttcc gccacgccgg
     5641 tgaacttgat cggcttgccg gtcacgtggc gtaccgacag cgcggcaccc ccgcgggcgt
     5701 cgccgtcgag cttggtcagc accacgccgg tgagcggaag tgcgtcgttg aacgcttttg
     5761 ccgtgttgat ggcatcctgg ccaagcatcg cgtcgaccac gaacagggtt tcgatcggct
     5821 tgaccgcggc gtgcagggcg gcgatctcct gcatcattgc
//
LOCUS       DATLGF010000314         7169 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_45771,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000314 DATLGF010000000
VERSION     DATLGF010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 7169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7169
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45771"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..167
                     /locus_tag="VM406_09615"
     CDS             <1..167
                     /locus_tag="VM406_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014899108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HVL76263.1"
                     /translation="PTLSETVQAWRDYLPRFLPLPHPSPRNQAWWKRRPWFEAELVPE
                     LRRRVKALLD"
     gene            179..673
                     /locus_tag="VM406_09620"
     CDS             179..673
                     /locus_tag="VM406_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HVL76264.1"
                     /translation="MSEQIDIKLPDSAQRVSDLLKRLGHHGRIVMLPDTGKTSAEAAA
                     GLGCSVAEIAKSIVFRRLADDAAVMVVASGANRVDEAKVAAIVGALGKADAKFVRERI
                     GYAIGGVCPVGHLEKTVMLIDQDLLGFDSVWAAAGHPHAVFNLTPKQLLELTGAPIAD
                     VALR"
     gene            694..1656
                     /locus_tag="VM406_09625"
     CDS             694..1656
                     /locus_tag="VM406_09625"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HVL76265.1"
                     /translation="MTTLQTIIAVIAAVALFLYALQGFSRELQVAGGDTLREWLGRVT
                     SNRWRAFAVGGVSTAVVQSSSAVTSLAVALVDARVITFGASLAIMLGAHVGTTATAWL
                     VSFKLTGIGPLFIVLGTLLSMMPKRIAIFGKAAFYFGLVFFTLDLIGSELRPLREHGM
                     LAEWLQLAQVPILGILTGIVITALVQSSSVTTGLAIVLVQQGMLAPEAAIAIAMGANI
                     GTSTTALIASTAMKQSARRAAVANLGFNLVGVTLFFPFIPAFAAAVIDASASPGIAVA
                     AAHLIFNVIVSTLFMLVLPRVEALLAERFTNDAEVEAINRHQTP"
     gene            1666..2094
                     /locus_tag="VM406_09630"
     CDS             1666..2094
                     /locus_tag="VM406_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HVL76266.1"
                     /translation="MRETLKPGLRFEWSYVVPPRATVPQLYHDTAFCREMPDVLATGY
                     MVGMMELACVNGIMPHVDWPREQSLGVLVNFRHLAATPPGMTLTIRGELLEVDGRQLR
                     FRVEAWDGIDKIAEGEHERMVITPERFNEKLAQKKEKAGL"
     gene            2091..2285
                     /locus_tag="VM406_09635"
     CDS             2091..2285
                     /locus_tag="VM406_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HVL76267.1"
                     /translation="MKQPASEQTPVPSPCISICRMDPKTGLCEGCLRTIEEIANWGSA
                     GDDYKRAVWQEIARRSGFTD"
     gene            complement(2304..2795)
                     /locus_tag="VM406_09640"
     CDS             complement(2304..2795)
                     /locus_tag="VM406_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76268.1"
                     /translation="MDPTDVLTKTPAGQDEVATRARQLPAKLRSLLIMVDGKRSVGEM
                     LAHHPAADEARASLAALLEAGLLALVPKPAAAPSPAAAPAAAAPALKGNIATVKKYIC
                     TALHDALGPDADLFTPRVEAATSLEALMTQAEKLREVLRGASGAKRADAFWTQLGTLV
                     TSE"
     gene            2881..3510
                     /locus_tag="VM406_09645"
     CDS             2881..3510
                     /locus_tag="VM406_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HVL76269.1"
                     /translation="MRKIDWYFDFISPFSYLQCERLHTLGAGVEVRYKPVLFAGLLAH
                     WDNKGPAEIAPKRLWTFEHCAWLAHRHGIPMRMPAHHPFNPLPLLRLCLALGATSDVV
                     ERLFRFVWRDGHLPTDAPAWEALLQELHATPQMLDDPAVKDRLRGNGEDAIAAGVFGV
                     PSAVVDNRVFWGLDATDMLAAYLNGDPFFQSELLRSAQTLPQGLQRPRK"
     gene            3512..3961
                     /locus_tag="VM406_09650"
     CDS             3512..3961
                     /locus_tag="VM406_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76270.1"
                     /translation="MTPKFYRPAKTVTGPSYSLWFKLLATVVTAVLLGYGINIATRFP
                     VMDYGFWVKMLLITSALMLAVSYYWFLKATTTIDENGITQTGMYNRHVEWRDVRSVKM
                     IGVPFMSAIFPPRLVVRTGVAFATFNGGTREILIEFANIALTYQLRK"
     gene            3958..4863
                     /locus_tag="VM406_09655"
     CDS             3958..4863
                     /locus_tag="VM406_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVL76271.1"
                     /translation="MNLPPSMRVFERGWLSSNNILFITPGETALVDSGYCTHAEQTLA
                     LVQSALGGRRLDRLVNTHLHSDHCGGNALLQRSYGCRTTIPVDEAQAVRAWDEAQLTF
                     AATGQRCERFGFDDTFAPGDVLELGGMAWKALAAPGHDPHALLLHCEAEGILISADAL
                     WENGFGVIFPELQGESGFAEQRAVLDLIAQLDLRLVIPGHGRPFTDAKAALQRAYSRL
                     DYLTADPQRNPRHAMKVLVAFLLLERQRMELDAIVEIIGASRLMRAAASQLHPDTGAL
                     VAELAGELVRAGAARMEGNALLNVR"
     gene            5280..6518
                     /locus_tag="VM406_09660"
     CDS             5280..6518
                     /locus_tag="VM406_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL76272.1"
                     /translation="MRAFAQGLFAASILFAAGAAPATAQQSQQAQQGKLSDNLVRIGV
                     LTDITGVYADISGRGAVEAVRMAVEDFGGTMFGRPIEVVFADHQNKADVGAAKAREWF
                     DVGGVDMINDLANSGVALAVANVAKEKKRHIIVNNASNVGITNSHCSPYAVHYTYDAW
                     SLANGTGRSIVENGGDTWFFLTVDFAFGVGLEQQVSDVVRANKGRVVGAARHPLGTTD
                     FSSYILQAQASRAKIIGLASTGNDTINAIKAAHEFGATRNQKLAALLLWINDVHALGL
                     DVAQGLQLTNAWYWDMNDETRAFAKRFQQRVGYMPNMAHAGDYSSTLHYLRAVQAAGT
                     DDADAVSAKMRELRINDMFTKNGYIRKDGRMVHDMYLWEVKKPSESKGPWDYLKPVKT
                     IPAEQAFMPLEQSSCALVRN"
     gene            6567..7169
                     /locus_tag="VM406_09665"
                     /pseudo
     CDS             6567..7169
                     /locus_tag="VM406_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833009.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT         1208 a         2351 c         2361 g         1249 t
ORIGIN      
        1 ggccgacgct gtccgagacc gtacaagcct ggcgcgacta cctgccgcgc ttcctgccgc
       61 tgccgcatcc tagcccgcgc aatcaggcgt ggtggaagcg gcggccgtgg ttcgaggcgg
      121 aactggtccc cgagttgcgc aggcgcgtca aagccctgct ggattgatcc ggaatttcat
      181 gagcgaacag atagacatca agcttcccga cagcgcgcag cgcgtctccg acctgctgaa
      241 gcgcctgggc catcacggcc gcatcgtgat gctgcctgac accggcaaga catccgccga
      301 agccgcggcc ggcctcggct gcagcgtggc cgagatcgcc aagtccatcg tgttccgccg
      361 gcttgccgat gatgcggcgg tgatggtggt cgccagcggc gcgaaccgcg tggacgaggc
      421 caaggtggcg gccatcgtcg gtgcgctcgg caaggcggat gcgaagttcg tgcgcgaacg
      481 tatcggctat gcaatcggcg gcgtgtgccc ggtcgggcat ctggagaaga cggtgatgct
      541 gatcgaccag gatctgctcg gcttcgacag cgtgtgggcg gccgccgggc atccgcacgc
      601 ggtgttcaat ctgacgccga agcagctgct ggagctgacc ggcgcgccga tcgcggatgt
      661 ggcgctgcgc taggctgagt tgagttcata ttcatgacca ccctgcaaac catcatcgcc
      721 gtcatcgccg ccgttgcgct gttcctctac gcgctgcagg gcttttcacg cgagctgcag
      781 gtcgcgggcg gcgacacgct gcgcgagtgg ctgggccggg tcacgtcgaa ccgctggcgc
      841 gcattcgctg tcggcggcgt ctccactgcc gtcgtgcagt cgagcagcgc ggtcacttca
      901 ctggccgtcg cgctggtgga cgcgcgggtg atcaccttcg gcgcaagcct ggcgatcatg
      961 cttggcgccc atgtcggcac caccgccacc gcctggttgg tgtcgttcaa gctgaccggc
     1021 atcgggccgc tgttcattgt gctcggcacc ttgctgtcga tgatgccgaa acgcattgcg
     1081 atcttcggca aggccgcgtt ctatttcggt ctcgtgttct tcacgctgga tttgatcggc
     1141 tccgagctgc gcccgctgcg cgagcacggc atgctggccg aatggctgca gcttgcgcaa
     1201 gtgccgatcc tcggcatcct gaccggcatc gtgatcaccg cgctggtgca atcgagcagc
     1261 gtgaccaccg gcctggccat cgtgctggtg cagcagggga tgctggcgcc ggaggcggca
     1321 atcgccatcg cgatgggcgc caacatcggc acctcgacga ccgcgctgat cgccagcacc
     1381 gcgatgaagc agtccgcccg ccgcgccgct gtcgccaatc tcggattcaa cctggtgggg
     1441 gtgacgttgt tcttcccgtt catcccggcc ttcgcggccg ccgtcatcga tgcgtcggcc
     1501 agccccggca tcgcggtggc ggccgcccac ctgattttca acgtgatcgt gtccaccctg
     1561 ttcatgctgg tgctgccgcg cgtcgaagcc ttgctcgccg agcgcttcac gaacgatgcg
     1621 gaagtcgagg cgataaacag gcaccagacc ccgtagggag acaacatgcg cgaaacactc
     1681 aagccgggac tgcgtttcga atggagctat gttgtgccgc cgcgcgcgac cgtcccccag
     1741 ctctaccacg acaccgcgtt ctgtcgcgag atgccggacg tgctggcgac cggttacatg
     1801 gtcggcatga tggagttggc ctgcgtgaac ggcatcatgc cgcacgtcga ctggccgcgc
     1861 gagcagagcc tgggcgtgct ggtgaacttc cggcacctgg cggcgacgcc acccggcatg
     1921 acgctgacga tacggggcga actgttggag gtggatggac ggcaactgag atttcgcgtc
     1981 gaagcctggg acgggatcga caagatcgcc gaaggcgagc acgagcgcat ggtgatcacg
     2041 ccggaacgct tcaacgaaaa actggcgcag aaaaaggaaa aggcgggact atgaagcagc
     2101 cggcgagtga acagaccccg gtaccgtcgc cttgcatctc gatctgccgg atggatccga
     2161 agaccgggct gtgcgaaggc tgcctgcgca cgatcgagga gattgccaac tggggcagcg
     2221 cgggcgatga ctacaagcgc gcggtatggc aggaaatcgc ccgccgcagc ggattcacgg
     2281 attgaatgtg cgcgggcacc gtgtcattcg ctggtgacca gggtgccgag ctgggtccag
     2341 aacgcgtcgg cgcgcttggc gccggaggcg ccgcgcagca cttcgcgcag cttctcggcc
     2401 tgggtcatca gcgcttccag cgaggtggcc gcctcgaccc tgggggtgaa caggtccgcg
     2461 tccggtccga gcgcgtcgtg cagggccgtg cagatgtact tcttgaccgt ggcgatattg
     2521 cctttgagcg ccggtgcagc ggcagccggg gccgcggcgg gagatggagc ggcggcgggc
     2581 ttgggcacca gtgccagcaa gccggcctcg agcagcgcgg ccaggctggc ccgggcttcg
     2641 tcggcggcgg ggtggtgggc cagcatctcg ccgacgctgc gcttgccgtc caccatgatc
     2701 aggagcgatc gcagcttggc cgggagctgg cgcgcgcggg tcgcgacctc gtcctggccg
     2761 gcgggggttt tcgtcagtac atcggtaggg tccatgcaca agtccttccg aaaacgcggt
     2821 ggcaccatat tggcccatgc cttgtttccg cgcaatagtt tcgtcatgag ggagtaacaa
     2881 atgcgcaaga tagactggta cttcgacttc atttccccgt tctcctacct ccagtgcgaa
     2941 cgtctccaca cccttggtgc gggcgtagaa gtgcgctaca agccggtgct gttcgccggc
     3001 ctgctcgcgc actgggacaa caagggcccg gccgaaatcg ccccgaagcg gctgtggacc
     3061 ttcgaacact gcgcgtggct ggcacacagg cacggcatcc cgatgcggat gccggcccat
     3121 catccgttca acccgctgcc gctcttgcgc ctgtgcctgg cgctgggcgc cacgtccgac
     3181 gtcgtcgagc ggctgttccg tttcgtctgg cgcgacggcc acctgccgac cgacgccccc
     3241 gcctgggagg cgctgctgca ggaactgcat gccacgccgc agatgctgga cgatcccgcg
     3301 gtcaaggacc gcctgcgcgg caacggcgaa gacgcgattg ccgccggcgt gtttggcgtc
     3361 cccagcgcgg tcgtggataa tcgggtgttc tgggggctgg atgccaccga catgctggcc
     3421 gcctacctca atggcgaccc gttcttccag tcggagctgc tgcgctccgc gcaaaccctg
     3481 ccgcaaggcc tgcaacgccc gcgaaagtga catgaccccg aagttctacc gccccgccaa
     3541 gaccgtcacc ggaccctcct acagcctgtg gttcaagctg cttgcgaccg tcgtgacggc
     3601 cgtgctgctc ggctacggca tcaacatcgc gacgcgcttt cctgtgatgg actacggctt
     3661 ctgggtcaag atgctgctga tcacgtcggc gctgatgctg gccgtcagct attactggtt
     3721 cctgaaggcg accacgacga tagacgagaa cggcatcacc cagaccggca tgtacaaccg
     3781 tcatgtcgag tggcgcgatg tgcgcagcgt gaagatgatc ggcgtgccgt tcatgagcgc
     3841 gatcttcccg ccgcgcctgg tggtgcgcac cggggtggcg ttcgccacct tcaacggcgg
     3901 cacgcgcgag atcctgatcg aattcgcgaa cattgcactt acctaccagc tcaggaaatg
     3961 aacctgcccc cttcgatgcg cgtgttcgag cgcggctggc tgtcgtcgaa caacatcctt
     4021 ttcatcaccc ccggggagac cgcgctggtc gacagcggct actgcacgca tgccgagcag
     4081 acgctggcgc tggtgcaaag cgcgctcggc ggtcgcaggc tggaccggct ggtcaacacc
     4141 cacctgcatt ccgaccattg cggcggcaac gcgctcttgc agcgcagcta cggctgccgc
     4201 acgacgatcc cggtcgacga ggcgcaggcg gtgcgcgcat gggacgaggc gcagcttacc
     4261 tttgccgcca ctggccagcg ctgcgagcgc ttcggcttcg acgacacctt cgcgcccggc
     4321 gatgtgctgg aactgggcgg catggcatgg aaggcgctgg cagcgccggg ccacgatccg
     4381 catgcattgc tgctgcactg cgaagccgaa ggcatcctga tctcggccga tgcgctgtgg
     4441 gaaaacggtt tcggcgtgat cttccccgag ctgcagggcg aatccggctt cgccgagcag
     4501 cgcgcggtgc tggacctgat cgcgcagctg gacctgcggc tggtcattcc cggccacggc
     4561 cggccgttca ccgacgcgaa agccgcactg cagcgcgcct acagccggct cgattacctg
     4621 actgccgatc cgcaacgcaa tccgcgccat gcgatgaagg tgctggtggc cttcctgctg
     4681 ctggagcgcc agcgcatgga gctcgatgcg atcgtcgaga tcatcggcgc gtcacggttg
     4741 atgcgcgcgg cggccagcca gctgcatccc gacacgggcg cgcttgtggc cgaactggcc
     4801 ggcgagctcg tgcgtgcggg tgccgcgcgc atggaaggca atgcgctgtt gaacgtgcgt
     4861 tagcgcgagt gcctcgcgcc gtcgctgggc ggaaaccgat ggcgttcagt tcagcgatta
     4921 ctcttgccat gtcgttcacc tcggcggccc cccgcgcagg cgggaaccca gttcgaacgc
     4981 ccgtcgtact gcttgcgact gaactgggtt cccgccttcg cgggagcgac atgacatggt
     5041 tcctcgctta actgaacggc atcccgcctt tccccttacc gccgcaactc gcacccgcat
     5101 ctcgccaatc gccgaccgcg cccgccttgc cgccgggcac acccagccac acgcgaaatg
     5161 tgtattttga gtatttccct gtgcctgcgc ggtatggatt tgccacaccc caccgcctaa
     5221 catcattcaa cactcggcga tccggctcgg gtgcatgcga ataacaaagg agacacacta
     5281 tgcgagcgtt tgcgcaggga ctctttgcgg cctcgattct gtttgcagcc ggcgccgcac
     5341 cggccaccgc ccaacagtca caacaggccc agcagggcaa gctttccgac aacctggtgc
     5401 gcatcggcgt gctgaccgac atcaccggcg tgtacgcgga tatctccggc cgcggggcgg
     5461 tggaagccgt gaggatggcg gtcgaggact tcggcggcac gatgttcggt cgtccgatcg
     5521 aggtggtgtt cgccgaccac cagaacaagg ccgatgtggg cgccgcgaag gcccgcgaat
     5581 ggtttgacgt cggcggcgtg gacatgatca acgacctggc caattcgggt gtggcgctcg
     5641 cggtggcgaa cgtggcgaag gaaaagaagc gtcacatcat cgtcaacaat gcttccaacg
     5701 tcggcatcac caacagccat tgctcgccgt atgcggtgca ttacacgtat gacgcgtggt
     5761 cgctggccaa cggcaccggc cgctcgatcg tggagaacgg cggcgacacc tggttcttcc
     5821 tgacggtgga cttcgcgttt ggcgtcggcc tggagcaaca ggtgtccgac gtggtgcgcg
     5881 ccaacaaggg ccgcgtggtc ggcgccgccc gccatccgct gggcacgacc gacttctcct
     5941 cctacatcct gcaggcacag gcttcgcgcg cgaagatcat cggcctggcc tcaaccggca
     6001 acgacacgat caacgcgatc aaggccgcgc acgagttcgg cgccacgcgc aaccagaagc
     6061 tggccgcgct gctgctgtgg atcaacgatg tgcatgcgct cggtctggac gtggcccagg
     6121 gtctgcagct gaccaatgcg tggtactggg acatgaacga cgagacccgt gcattcgcga
     6181 agcgcttcca gcagcgcgtc ggctacatgc cgaacatggc gcatgccggc gactattcgt
     6241 cgacgctcca ctatctgcgc gcggtgcagg cagccggcac cgacgatgcg gatgccgtgt
     6301 cggccaagat gcgcgagctg cgcatcaacg acatgttcac caagaacggc tatatccgca
     6361 aggatggccg catggtgcac gacatgtatc tgtgggaagt gaagaagccg tccgagtcga
     6421 agggcccgtg ggactatctg aagccggtca agaccatccc ggcggagcag gccttcatgc
     6481 cgctcgagca aagcagctgc gcgctggtgc gcaactgagc ggtccgtcct gatgcctgtc
     6541 tgtctcatcc tcgtacggga ggacctgtga cgaccatcct ggaagcgcgc ggcctgtcac
     6601 gcagtttcgc cggctccacc gcggtcgacg gcgtggacct gaacatcagg gagggcagca
     6661 tccacgcgct gatcggcccg aacggagcag gcaagacgac ctgcttcaac ctgctcacca
     6721 ggttcctcga gccgaccgcg ggccgcatcg ccttcgccgg gcgcgacatc accggcatca
     6781 ggcccgatgt cgtggcccgc tccgggttgg tgcgctcctt ccagatctcg gctgtgtttt
     6841 ctcagctgag cgtgcacgaa aacgtgcgcc tggccgtgca acgccatcgc ggccattcgt
     6901 tcgactggtg gcgcagcgac aaggtgctgc gcgagcatga ccggcgcgcc gacgcgctgc
     6961 tggaagaagt cggcctcggc gactatgccc ggcaccgcgc cggctaactc tcctacggcc
     7021 gcaagcgtgc attggaactg gcgaccacgc tggccctcga gccgcgcatg ctgctgctcg
     7081 acgagccgat ggccggcatg ggtgtcgaag acatcacgca ggcaaccgcg ttgatccgca
     7141 aggccgccca gggccgcacc gtgctgatg
//
LOCUS       DATLGF010000315         5350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_29658,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000315 DATLGF010000000
VERSION     DATLGF010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29658"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VM406_09670"
     CDS             complement(<1..614)
                     /locus_tag="VM406_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HVL76273.1"
                     /translation="MADFTSGFWSIYIAVLTLLGIAGCAVLLWVQAKAKIVIPEGEQA
                     EPMGHSWDEGLHELNNPMPRWWMWLFIITIVFSLVYLVLYPGLGAYAGKLGWQSAGQY
                     EAEQRKADADFGPIFAAYQQADLKQVAADPQARAIGERLFMTYCAQCHASDARGSKGF
                     PNLTDDDWLYGGEHETIKQTIANGRTGVMPPMAAAVGSAEDVRNL"
     gene            complement(607..786)
                     /locus_tag="VM406_09675"
     CDS             complement(607..786)
                     /locus_tag="VM406_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HVL76274.1"
                     /translation="MSIESLIFDARNIVTVLSFASFLGIVFWAYSASRKPAFEEAANL
                     PFADEPNSRGEEQHG"
     gene            complement(790..1407)
                     /gene="ccoO"
                     /locus_tag="VM406_09680"
     CDS             complement(790..1407)
                     /gene="ccoO"
                     /locus_tag="VM406_09680"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HVL76275.1"
                     /translation="MKKFTHEKIEKNPWLLIGLVILVVSVGGLVEILPLFFQKSTTEP
                     VAGLQPYSALRLAGRDVYIKEGCYNCHSQMIRPFRAETERYGHYSVAAEFVYDRPFQW
                     GSKRTGPDLARVGGRYSDEWHRIHLDNPRDVVPESNMPAYPWLAKTPLDPASIQPKMR
                     ALQKLGHPYSEEEIAAAPKELEGKNEQDALVAYLQGLGVLIKAKR"
     gene            complement(1424..2848)
                     /gene="ccoN"
                     /locus_tag="VM406_09685"
     CDS             complement(1424..2848)
                     /gene="ccoN"
                     /locus_tag="VM406_09685"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HVL76276.1"
                     /translation="MEPSLNYNYKVVKQFAIATVVWGVVGMLVGVFIAAQLAWPALNF
                     DIPWLSYGRLRPLHTNAVIFAFGGCALFATSYYVVQRTCQVRLFSDAIAAFTFWGWQL
                     VIVAAAISLPLGYTSGKEYAELEWPIDILITLVWVSYAVVFFGTIVKRKVAHIYVANW
                     FFGAFILTVAILHLVNSAAMPVSAMKSYSAYAGVQDAMIQWWYGHNAVGFFLTAGFLG
                     MMYYFIPKQADRPVYSYRLSIVHFWALIFTYMWAGPHHLHYTALPDWAQSLGMVFSLI
                     LLAPSWGGMINGVMTLSGAWHKLRTDPILKFLIVSLSFYGMATFEGPMMSIKTVNALS
                     HYTDWTVGHVHAGALGWVGFISMGAIYYLLPRLVGKQQMYSTQMIETHFWIATIGIVL
                     YIASMWIAGVMQGLMWRAVNADGTLTYTFVESVKATYPYYVIRFVGGLLYFGGMVLMA
                     WNTVMTIRDPKPVDTRIPALAAHA"
     gene            complement(3037..3261)
                     /gene="ccoS"
                     /locus_tag="VM406_09690"
     CDS             complement(3037..3261)
                     /gene="ccoS"
                     /locus_tag="VM406_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HVL76277.1"
                     /translation="MEALYLLVPLSVAVVFLAIWIFFRASDSGQFDDLVGPGMRILQD
                     DDRTAPAPAEHPLSGSAAGPARSHERKVEA"
     gene            complement(3272..>5350)
                     /locus_tag="VM406_09695"
     CDS             complement(3272..>5350)
                     /locus_tag="VM406_09695"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HVL76278.1"
                     /translation="HVRWDDAQCKPSDILRAVRAVGYTAYPFDAVRHDEQLQRASKTL
                     FRQVFIAGLSMMQVMMYAFPVYVAGEGEMDFDMLALMNWASLLLTIPAVFYSALPFFR
                     GAWANLKARSLGMDVPVALGIGAAFAGSVVATVTGEGEVWYDSVTMFIFLLLCSRYLE
                     LMARRKAAFALERLQHGLPASASRMPAYPSDRTTEIVPAARLAAGEYLLVKPGEAVAA
                     DCVIVEGSTSADLSLLTGESQPQRKSVGDSLPGGAVNATQAVVVQVTALARDSTLSTL
                     VKLIERAGQAKPQRAMWADRVAAWFVAGLLLFATLIFFAWQWIDPARAWPIAIAVLVV
                     SCPCALSMATPSALAAATDRLVRQGVLVVQPHVIETLHRITHVVFDKTGTLTLGKPVV
                     RATRVLGSEPESWCLQAAAALEASSAHPLALALLDAARAHGDSGLVSTDLLHEAGRGV
                     EGTVDGRRLRLGSAGWVGQIAGGEPPKAGDFTAVYLGTDGNWLARFELADALRADARD
                     VVAHFRSQGKQVVLLSGDAPGVTARIAAELGIEQAQGGFLPQQKLEYVQGLQQGGAVV
                     AMVGDGVNDAAVLRAADVSFAMGSGAALAQTHADTVLLSPRLSAVVDASRAANQTMAV
                     IRQNLFWASVYNVTAIPAAAVGLLNPWMAGVGMAASSAVVVLNALRLCRVPRSRASTT
                     PVQLAGPASA"
BASE COUNT          988 a         1774 c         1717 g          871 t
ORIGIN      
        1 aggttgcgca cgtcctcggc cgagccgacg gctgccgcca tcgggggcat cacgccggtg
       61 cggccgttcg cgatcgtctg cttgatggtc tcgtgctcgc cgccgtagag ccagtcgtcg
      121 tcggtcaggt tcgggaagcc cttgctgccg cgcgcgtccg atgcatggca ttgcgcgcag
      181 taggtcatga acaggcgctc gccgatcgca cgtgcctgcg ggtccgctgc cacctgcttc
      241 aggtcggcct gctggtaggc ggcgaagatc ggtccgaaat cggcatcggc cttgcgctgc
      301 tcggcttcgt actggccggc cgactgccag ccgagcttgc cggcataggc gccgaggccc
      361 ggatacagca ccagatagac caggctgaac acgatggtga tgatgaacag ccacatccac
      421 cagcgcggca tcgggttgtt cagctcgtgc aacccctcat cccagctatg ccccatcggc
      481 tcggcttgct cgccctccgg gatcacgatc ttcgccttcg cctgcaccca cagcagcact
      541 gcacagccgg cgatgccaag cagggtcagc actgcgatgt agatgctcca gaaaccactc
      601 gtaaagtcag ccatgttgct cctccccgcg ggagttcggt tcgtccgcga acggcaggtt
      661 tgccgcttcc tcgaatgccg gcttgcggct tgcactgtat gcccagaaca cgatgccgag
      721 gaagcttgcg aagctcagca cggttacgat gttgcgggcg tcgaaaatca gggattcgat
      781 gctcatgatt tacctctttg ccttgatcag cacgcccagg ccctgcaggt aggcgaccag
      841 cgcgtcctgc tcgttcttgc cttccagttc cttcggcgcg gctgcgattt cctcttcgct
      901 gtacggatgg ccgagctttt gcagcgcgcg catcttcggc tggatcgacg ccggatcgag
      961 cggcgtcttc gccagccacg gataggcggg catgttcgac tcgggcacca cgtcgcgcgg
     1021 gttgtccagg tggatgcggt gccactcgtc cgagtagcgg ccgcccacgc gcgccagatc
     1081 cgggccggtg cgcttggagc cccactggaa cgggcggtcg tagacgaact cggcggcgac
     1141 cgagtagtgg ccgtagcgct cggtctcggc gcggaacgga cggatcatct gcgaatggca
     1201 gttgtagcag ccttccttga tgtagacgtc gcgcccggcc aggcgcagcg ccgagtacgg
     1261 ctgcaggccc gccaccggct cggtggtcga cttctggaag aacagcggca ggatctcgac
     1321 cagaccgccc acgctcacca ccagaatcac cagcccgatc aggagccagg gattcttttc
     1381 gatcttttcg tgcgtaaact tcttcattgc gatgctccag gtgttaagcg tgtgcggcca
     1441 gcgccgggat acgggtatcc accggcttcg gatcgcggat agtcatgacc gtgttccatg
     1501 ccatcagcac catgccgccg aagtacagca ggccgccgac gaagcgaatc acgtagtacg
     1561 gataggtggc cttcaccgat tccacgaagg tgtaggtcaa ggtgccgtcg gcgttcaccg
     1621 cgcgccacat caggccctgc atcacgccgg cgatccacat cgaggcgatg tagagcacga
     1681 tgccgatggt cgcgatccag aagtgggtct cgatcatctg cgtgctgtac atctgctgct
     1741 tgccgaccag gcgcggcagc aggtagtaga ttgcgcccat cgagatgaag ccgacccagc
     1801 ccagcgcgcc cgcatgcacg tggcccacgg tccagtcggt gtagtgggac agcgcgttga
     1861 cggtcttgat cgacatcatc gggccctcga aggtggccat gccgtagaac gacagcgaga
     1921 cgatcaggaa cttcaggatc gggtcggtgc gcagcttgtg ccatgcgccg gacagcgtca
     1981 tcacgccgtt gatcatgccg ccccaggaag gcgccagcag gatcagcgaa aacaccatgc
     2041 ccagcgactg cgcccagtcg ggcagcgcgg tgtagtgcag gtggtgcgga cctgcccaca
     2101 tgtaggtgaa gatcagcgcc cagaagtgca cgatcgacag gcggtacgaa tacaccgggc
     2161 gatcggcctg cttcgggatg aagtagtaca tcatcccgag aaagccggcg gtcaggaaga
     2221 agccgaccgc gttatggccg taccaccact ggatcatcgc gtcctgcacg ccggcatagg
     2281 ccgagtacga cttcatcgcc gacaccggca tcgcggcgct gttgacgaga tggaggatcg
     2341 ccaccgtcag gatgaacgcg ccgaagaacc agttggccac gtagatgtgc gcgaccttgc
     2401 gcttgacgat ggtgccgaag aacacgactg cataggacac ccagacgagc gtgatcagga
     2461 tgtcgatcgg ccattcgagt tcggcgtatt ccttgccgga ggtgtagccc agcggcaggg
     2521 agatggccgc ggcgacaatg accagttgcc agccccagaa cgtaaaggcg gcgatggcgt
     2581 cggaaaacag gcgtacctgg caggtacgct ggacgacgta gtacgaggtg gcgaacagcg
     2641 cgcagccgcc gaacgcgaag atgactgcgt tggtgtgcaa gggccgcagg cggccgtagc
     2701 tcagccatgg tatgtcgaag ttcagggctg gccaggccag ctgggcagcg atgaagacgc
     2761 cgaccagcat gcccaccacg ccccacacta cggtggcgat cgcgaattgc ttcacgacct
     2821 tgtagttgta gttcagtgag ggttccacgt actttctccc agtgtgcgaa tggatggaag
     2881 gcagagtacg gatgcagcac cgcaaaaact ttgactcaag tcaaaatgct ccgagagcac
     2941 ggcgggcgag ggaaatgcgc aggattagag aggaatggca gtgccgacag tgcgcgccgg
     3001 cgcacctcag gaaggggcga aagcacgcag caaacgtcag gcttcgacct tgcgctcatg
     3061 cgaacgcgcg ggcccggctg ccgagccgga cagagggtgt tctgcgggcg caggtgcggt
     3121 acggtcgtca tcctgcagga tgcgcatccc gggcccgaca aggtcgtcga actgcccgct
     3181 gtcggatgcg cggaagaaga tccagattgc caggaacacc acggcaacac tgagcggaac
     3241 gagcagatac aaggcttcca tcgcttttcc cctatgcgct cgccggtccc gccagctgca
     3301 ccggcgtggt cgatgcacgg ctgcgcggca cgcggcacaa gcgcagcgcg ttgagcacga
     3361 cgacggccga gctcgcggcc atccctacgc cggccatcca cggattgagc aggccgacgg
     3421 cggccgccgg tatcgcggtc acgttgtaga cgctggccca gaacaggttc tggcgtatca
     3481 ccgccatcgt ctggtttgcc gcacgactgg cgtcgaccac ggccgacagc cgcggcgaca
     3541 acagcaccgt gtccgcatgt gtctgcgcca gcgccgcgcc gctgcccatt gcaaacgaca
     3601 catcggccgc gcgcagcact gccgcatcgt tgacgccgtc gccgaccatc gcgaccaccg
     3661 ccccgccctg ctgcaagcct tgcacgtact ccagcttctg ctgcgggaga aagcccccct
     3721 gcgcctgttc gatcccgagc tcggccgcga tgcgcgcggt cacgccgggc gcgtcgccgc
     3781 tcagcagaac gacctgcttg ccctgcgagc ggaagtgcgc aacgacatcg cgcgcatcgg
     3841 cgcgcagcgc gtcggcgagt tcgaagcgtg caagccaatt gccgtcggtg ccgagataga
     3901 cggcggtgaa atcgccggcc ttgggcggct cgccgcctgc gatctgcccg acccagcccg
     3961 cagaaccaag ccgtagccgt cgcccatcca ctgtcccctc gacgccgcgt ccggcctcgt
     4021 gcagcaggtc cgtggacacc agcccgctgt cgccgtgtgc acgcgccgca tccagcaggg
     4081 ccagcgcgag cgggtgcgcg ctcgacgcct ccagcgcagc tgcggcctgc aggcaccagc
     4141 tttccggctc gctgccgagc acccgcgtcg cccgcacgac cggcttgccg agcgtaagcg
     4201 tgccggtctt gtcgaaaacc acgtgggtaa tcctgtgcag ggtctcgatc acatgcggct
     4261 gcacgaccag aacgccctgg cgcaccagcc ggtcggtcgc ggccgccagc gcgctcggcg
     4321 tggccatcga cagcgcgcag ggacatgaca cgaccagtac cgcgatcgcg atcggccatg
     4381 cgcgcgccgg gtctatccat tgccaggcga agaagatcag cgtggcaaac agcagcaggc
     4441 ccgcgacaaa ccaggccgcg acacggtcgg cccacatcgc ccgctgcggc ttggcctgcc
     4501 ctgcccgctc gatcagcttg accagcgtgg acaaggtgct gtcgcgcgcg agcgcggtga
     4561 cctgcacgac gacggcctgc gtcgcattga ccgcaccgcc cggcaggcta tcgccgacgc
     4621 tcttgcgttg cggctggctt tcgccggtga gcagcgaaag atccgcgctg gtgctgcctt
     4681 cgacgatcac gcagtcggcg gcgaccgctt cacccggctt gaccagcaga tactcgccgg
     4741 ccgcgagccg cgccgccggc acgatttcgg ttgttctgtc ggacggatat gccggcatgc
     4801 ggctggccga tgccggcaag ccatgctgca ggcgctccag cgcaaacgcc gccttgcgcc
     4861 ttgccatcag ttccaggtag cggctgcaca gcagcaggaa gatgaacatc gtcaccgagt
     4921 cgtaccagac ctcgccctcc ccggtgacgg tggcgacgac gctgccggcg aaggccgcgc
     4981 cgattcctag cgcaaccggc acatccatgc ccagcgagcg cgccttcagg ttggcccagg
     5041 cgccgcgaaa gaacggcaac gccgaataga acaccgcggg aatcgtcaac agcaagctgg
     5101 cccagttcat cagcgccagc atgtcgaaat ccatctcgcc ttcaccggcc acgtagaccg
     5161 ggaatgcata catcatcacc tgcatcatcg acaagccggc gatgaagacc tggcggaaca
     5221 gggttttcga tgcgcgctgc aactgttcgt cgtggcgcac cgcgtcgaac ggataggcgg
     5281 tgtagccgac cgcgcgcacc gcgcgcagga tgtcgctcgg cttgcattgc gcgtcgtccc
     5341 agcgcacatg
//
LOCUS       DATLGF010000316         2738 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_72721,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000316 DATLGF010000000
VERSION     DATLGF010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72721"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VM406_09700"
     CDS             <1..387
                     /locus_tag="VM406_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVL76279.1"
                     /translation="ILKGRTGVMPPMAAALGSDKDVEHVAHYVLSLSGSASDPIKAVF
                     GKSKFGACVACHGADGKGNQALGAPNLTDKTWLYGGSVASIMETIRSGRTNSMPGFED
                     FLGDAKVHVIAAYVWSLSNRQELAQK"
     gene            533..1933
                     /gene="ccoG"
                     /locus_tag="VM406_09705"
     CDS             533..1933
                     /gene="ccoG"
                     /locus_tag="VM406_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HVL76280.1"
                     /translation="MGDPAPKVQIIRMYEARQEIYPREVKGRYANLRWFFVWLTQAIF
                     YGLPWLAWNDRQAVLFDLGARKFYIFGLVLWPQDFIYLALLLIISAYALFLFTAVFGR
                     VWCGFSCPQTVYTEIFMWVERRIEGDRSARMRLDRQPWNAQKIARKSAKHFVWGAIAL
                     WTGFTFVGYFSPIRELGQSVVSLGLGPWETFWILFYGFATYGNAGWMREQVCKYMCPY
                     ARFQSAMFDRDTLIVSYDATRGEPRGARAKSKAAQASSLGDCIDCSMCVQVCPVGIDI
                     RDGLQYDCISCAGCVDACNNVMDKIGAPRGLIRFSTDNALRQRFSLREIVRRTLRPRV
                     LVYSTILVLIVAAFSASLYWRTPVKLDVIRDRGVMAREVEDGLIENVYRLQVMNTTER
                     SQRLRITVTGIDGIRLDTPDLIEVEGTTTQAVPVRVRIDPAAGERGSNTIFFELQSRD
                     EAWLHVKEKAAFIVPR"
     gene            1954..2493
                     /locus_tag="VM406_09710"
     CDS             1954..2493
                     /locus_tag="VM406_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HVL76281.1"
                     /translation="MQASAPITNHNRPAPWYRHRWPWLLMIGPAAVVVAGIHTTWIAF
                     SHQDALVTGDYYKQGKAINQDLRRAHAAAALKLEVNLRYDPAAGRLLGTVDSDGRPLE
                     SSLIVTLNHSTLPEKDVRLLVYADAEGRFAAPLPMLDMARWIVTVEDAQRDWRMAGRW
                     LWPQNPALQILAETPVLPD"
BASE COUNT          485 a          868 c          902 g          483 t
ORIGIN      
        1 atcctgaagg gccgcaccgg ggtgatgccg ccgatggccg cggcgctcgg ctcggacaag
       61 gacgtcgagc atgtcgcgca ttacgttctc agcctgtccg gctccgcttc cgacccgatc
      121 aaggcggtgt tcggcaagag caagttcggc gcctgcgtgg cctgccatgg agcagacggc
      181 aagggcaacc aggcgctcgg cgcccccaat ctgaccgaca agacctggct ctatggcggc
      241 agcgtggcat ccatcatgga aaccatccgc tccggccgca cgaacagcat gccgggcttc
      301 gaggatttcc tcggcgatgc gaaggtgcat gtgattgcag cgtatgtctg gagcctgtcg
      361 aaccggcagg agctggcgca gaagtaggcg cagtcatgca agcttccctg tctctccgat
      421 gaggcagggc gggggcggag ccgaccgggc gagtagcacg cagcggtccc gccaccgagc
      481 cagccgccgt cggcaatccg ccgccgcgcg cttgaacgag gtagtaagca aaatgggcga
      541 ccccgcaccc aaagtccaga tcatccgcat gtatgaagcg cggcaggaaa tctatccgcg
      601 cgaagtcaag ggcaggtatg cgaacctgcg ctggttcttc gtctggctca cgcaggcgat
      661 tttctacggc ctgccgtggc tcgcatggaa cgaccgccag gcggtgctgt tcgatctcgg
      721 tgcacgcaag ttctacatct tcggcctggt gctctggccg caggatttca tctatctggc
      781 gctgctgctg atcatcagcg cctacgccct gttcctgttc accgcggtgt tcggccgggt
      841 ctggtgcggc ttctcgtgcc cgcagaccgt gtacacagaa atcttcatgt gggtcgagcg
      901 acgcatcgaa ggcgaccgca gcgcccgcat gcgcctggac cgccagccat ggaacgcgca
      961 gaagatcgcc cgcaagtcgg ccaagcactt cgtctggggc gcgatcgcgc tatggaccgg
     1021 tttcaccttc gttggctact tctcgccgat ccgcgaactc ggccagtcgg tcgtctcgct
     1081 cggcctcggg ccgtgggaaa ccttctggat cctgttctac ggcttcgcca cctacggcaa
     1141 cgccggctgg atgcgcgagc aggtgtgcaa gtacatgtgc ccgtatgcgc gcttccagag
     1201 cgcaatgttc gaccgcgaca cgctgatcgt ttcgtacgat gcgacgcgcg gcgaaccgcg
     1261 cggggcgcgc gcgaaatcca aggcggcgca agcgtcgagc ctgggcgact gcatcgactg
     1321 ctcgatgtgc gtgcaggtct gtccggtggg catcgacatc cgcgacggcc tgcagtacga
     1381 ctgcatcagc tgcgccggct gcgtggacgc ctgcaacaac gtgatggaca agatcggcgc
     1441 gccgcgcggc ctgatccgct tctcgaccga caacgcgctg cgccagcgct tttcgctgcg
     1501 cgagatcgtc aggcgcacat tgcgcccgcg ggtcctggtc tacagcacga tcctggtgct
     1561 gatcgtcgcg gcgttctccg cttcgctcta ctggcgcacg ccggtgaagc tggacgtgat
     1621 ccgcgaccgc ggcgtgatgg cgcgcgaggt cgaggacgga ctgatcgaga acgtctaccg
     1681 gctgcaggtg atgaacacga ccgagcgctc ccagcggctg cggatcacgg tgaccggcat
     1741 cgacggcatc cgactggata cgcccgatct gatcgaggtg gaaggcacca cgacgcaggc
     1801 cgtaccggta cgcgtgcgca tcgatccggc ggccggagag cgcggctcga acacgatatt
     1861 ttttgaattg cagtcgcgcg acgaagcgtg gctgcacgtg aaggaaaaag cggcattcat
     1921 cgtgccgcgc tgacgggaaa acaggagttc atcatgcagg caagcgcacc cattacgaac
     1981 cacaaccggc cggccccctg gtacaggcac cgctggccgt ggctgttgat gatcggcccg
     2041 gcggcggtcg tcgtggccgg gattcacacg acctggatcg cgttctcgca ccaggatgcg
     2101 ctggtcaccg gcgactatta caagcaaggc aaggcgatca accaggacct gcgccgcgcg
     2161 catgcggccg ctgcgctgaa gctggaagtg aacctgcgct acgacccggc tgccggccgg
     2221 ctgctcggca ccgtcgacag cgacgggcgt ccgctcgaat cctcgctgat cgtcaccctc
     2281 aatcattcga cgctgcccga gaaggatgtg cgcctgctcg tgtacgccga tgccgaaggg
     2341 cgattcgccg ccccgctgcc gatgctggac atggcgcgct ggattgtcac agttgaagac
     2401 gcgcagcggg actggcgcat ggccgggcgc tggctgtggc cgcagaaccc cgcgctgcag
     2461 atcctggccg agacaccggt gctgccggat tgagcatatg cacgggccgg gttccttgtc
     2521 gaaaagtgcc acgccgggca ttctcacaaa atccaatcgg attcccgatt tcgggaatgc
     2581 ttcaaacggt cacgcctgca agagcgtgat cagggagact acaaggaggc aggggcaatg
     2641 ggacgcatgt taatggtcgt actctggccg tcgttcctgg tggcgattgt cgcggagggc
     2701 gtgttcttct cggtgttcga gccggccgag ctggtatg
//
LOCUS       DATLGF010000317         3412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_196042, whole genome shotgun sequence.
ACCESSION   DATLGF010000317 DATLGF010000000
VERSION     DATLGF010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196042"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3364)
                     /locus_tag="VM406_09715"
     CDS             complement(<1..3364)
                     /locus_tag="VM406_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HVL76282.1"
                     /translation="MNAPLTSGQRALLENISLDDKFTLERGRAFITGTQALVRLPMMQ
                     RQRDVRAGLNTAGFISGYRGSPLGNVDMTAMKARKYLEAHHVKFHPGMNEDLAATSIW
                     GTQQVNLFPGAKYDGVFSMWYGKGPGVDRCGDVFKHANMAGTDKHGGVLVLAGDDHAA
                     KSSTAAHQSEHILKACGIPVLYPSSVQEYLDYGLHGWAMSRYTGLWVAMKCVTDVVES
                     GASVELDPDRVQIVVPTEFELPPGGLNIRYPDAVLEQEARMNHYKWYAALEYVRVNKL
                     NRIIWDSPRARIGIITAGKSYLDTRQALADLGIDEAMAKEIGIRLYKIGMTWPLEAEG
                     VRNFAQGLDEILVVEEKRQILEYQLKEELYNWRDDVRPRVVGKFDDSGEWSGNHRKGH
                     GDWLLPATYELNPAQIARAIATRITKYYAGHPIEQRVRERVAYLEAKEAVLKVSAKPD
                     PAKDRIPHFCSGCPHNTSTKVPEGSRALAGIGCHYMVLWMDRQTATFTHMGAEGVTWV
                     GLAPFTEEKHVFANLGDGTYFHSGILAIRAAVSSKVNMTYKILYNDAVAMTGGQNVDG
                     PLDPAMISRQIAAEGVNPIVVVTDEPQKYPAGINWAPGVTIRHRDELDAVQKELREVQ
                     GVSAIIYDQTCASEKRRRRKKTGPDGKPLYPDPAKRAVINEAVCEGCGDCSVQSNCLS
                     VEPLETEFGRKRQINQSSCNKDFSCVKGFCPSFVTVEGGQLKKPKKAAVEADSGAKKL
                     PILDGSNLPQPTLPGTAEPYGILVTGVGGTGVVTVGQIIAMAAHAEGKGCSVLDMSGL
                     AQKGGPVMSHVRVADSPEVIHSTRVGTGAADLVIGCDLIVTGSRDALSRMGEGRTHAA
                     VNTTGAPTAAFVKNPNWKFPGDEAQAEIRRACGAEQVDLIDAGRVATALMGDSIATNM
                     FMLGYAWQKGWVPVSEAALMKAIELNGVSIDFNKQAFIWGRNAAHDWAAVERLAKAND
                     KAAQVIEFKRTPTLDELIERRSAFLAAYQNDAYAQQYRAFVEKVRHAEQETVGDGRAL
                     RLTEAVANYLSKLMAYKDEYEVARLHTDPDFHARIAAQFEGDYKLAFHLAPPALGRKN
                     ARGEPVKQRFGPAMLTGFKWLAKLKGLRGTALD"
BASE COUNT          502 a         1142 c         1105 g          663 t
ORIGIN      
        1 ggtcgagcgc ggtgccgcgc aggcccttca gtttcgcgag ccacttgaag ccggtgagca
       61 tcgccgggcc gaagcgctgc ttcaccggct cgccgcgcgc gttcttcctg ccgagcgcgg
      121 gcggggccag gtggaaggcg agcttgtagt cgccctcgaa ctgcgcggcg atccgggcgt
      181 ggaagtccgg gtcggtgtgc agccgcgcga cctcgtactc gtccttgtag gccatcagct
      241 tggacaggta gttggcaacc gcctcggtca ggcgcagcgc gcgcccatcg ccgaccgtct
      301 cctgctccgc atggcgcacc ttctcgacga acgcgcggta ctgctgcgcg taggcatcgt
      361 tctggtaggc cgccaggaac gcggaacggc gctcgatcag ttcgtcgagc gtcggcgtgc
      421 gcttgaactc gatcacctgg gccgccttgt cgttggcctt cgccaggcgc tcgaccgccg
      481 cccagtcatg cgcggcattg cgcccccaga tgaaggcctg cttgttgaag tcgatcgaca
      541 cgccattgag ttcgatcgcc ttcatcagcg cggcttccga caccggcacc cagcccttct
      601 gccatgcata gccgagcatg aacatgttgg tggcgatgga atcgcccatc agcgcggtcg
      661 cgacgcggcc ggcgtcgatc aggtcgacct gttccgctcc gcaggcacgg cggatctcgg
      721 cctgcgcttc gtcgccggga aacttccagt tcgggttctt cacgaaggcc gcggtcggcg
      781 cgccggtggt gttgaccgcc gcatgcgtgc gcccctcgcc catgcgcgag agcgcgtcac
      841 ggctgccggt gacgatcagg tcgcagccga tcaccaagtc agccgcgccg gtgcccacgc
      901 gggtcgaatg gatcacctcc ggcgaatcgg ccacgcgcac gtgcgacatc accggaccgc
      961 ccttctgcgc cagaccgctc atgtcgagca ccgagcagcc cttgccttcc gcatgcgcgg
     1021 ccatcgcgat gatctgcccg accgtcacca cgccggtgcc gcccacaccc gtcacgagta
     1081 tgccgtaagg ctcggcggtg cccggcagcg tcggctgcgg caggttgctg ccgtcgagta
     1141 tcggcagctt cttcgcgccg gaatcggcct ccaccgccgc cttcttcggc tttttcagct
     1201 gcccgccttc gacggtgacg aagctcgggc aaaagccctt cacgcaggag aagtccttgt
     1261 tgcaggaaga ctggttgatc tggcgcttgc gtccgaactc ggtttccagc ggctccaccg
     1321 acaggcagtt cgactgcacg ctgcagtcgc cgcagccttc gcacacggcc tcgttgatca
     1381 ccgcgcgctt ggccgggtcc gggtacagcg gcttgccgtc cgggccggtc ttcttgcggc
     1441 ggcgccgctt ctccgacgcg caggtctggt cgtagatgat cgccgagacg ccctgcacct
     1501 cgcgcagctc cttctgcacc gcgtcgagct cgtcgcgatg gcggatcgtc acgcccggcg
     1561 cccagttgat gccggccggg tacttctgcg gttcgtcggt gacgacgacg atcgggttca
     1621 ccccttcggc cgcaatctgg cgcgagatca ttgccgggtc cagcggtccg tcgacattct
     1681 ggccgccggt catcgccacc gcgtcgttgt aaaggatctt gtaggtcatg ttgaccttgc
     1741 tcgacaccgc agcccggatc gcgaggatgc ccgagtggaa atacgtgccg tcgccgagat
     1801 tggcgaacac gtgcttttct tcggtgaacg gcgcaaggcc cacccaggtg acaccctcgg
     1861 cgcccatgtg ggtgaaggtc gccgtctggc gatccatcca cagcaccatg tagtggcagc
     1921 cgatgccggc cagcgcgcgg ctgccttccg gcaccttggt ggacgtgttg tgcggacagc
     1981 cggagcagaa atgcgggatg cggtccttgg ccggatccgg cttggcggaa accttcagca
     2041 ccgcctcctt cgcttcgaga taggcaacgc gctccctgac gcgctgttcg atcgggtggc
     2101 cggcgtagta cttggtgatt cgagtggcga tcgcacgcgc gatctgcgcc gggttcagtt
     2161 cataggttgc cggcagcagc cagtcgccgt ggcccttgcg gtggttgccg ctccattcgc
     2221 cgctgtcgtc gaatttgccg accacacggg ggcgcacgtc gtcgcgccag ttgtacaact
     2281 cttccttcag ctggtattcg aggatctggc gcttctcctc gaccacgaga atctcgtcga
     2341 gtccctgcgc gaagttgcgc acgccttcgg cctcgagcgg ccaggtcatc ccgatcttgt
     2401 acaggcgaat gccgatttcc ttcgccatcg cctcgtcgat cccgagatcg gccagcgcct
     2461 ggcgtgtgtc gagataggac ttgcctgcgg tgatgatgcc gatgcgcgcg cgcgggctgt
     2521 cccagatgat gcggttgagc ttgttgacgc gcacatactc gagcgcggca taccacttgt
     2581 agtggttcat gcgcgcttcc tgctcgagca ccgcgtcggg gtagcggatg ttcaggccgc
     2641 ccggcggcag ttcgaattcg gtgggcacga cgatctgcac acgatccgga tcgagctcga
     2701 ccgaggcgcc ggactcgacc acgtcggtca cgcatttcat cgcgacccac agcccggtgt
     2761 agcgcgacat cgcccagccg tgcaggccgt agtccagata ttcctgcacc gaggacggat
     2821 acagcaccgg gatgccgcag gccttcagga tgtgttcgct ctggtgcgca gcggtcgagg
     2881 atttcgccgc gtgatcgtcg ccggccagca ccagcacgcc gccgtgcttg tcggttcccg
     2941 ccatgttggc gtgcttgaac acgtcgccgc agcgatccac gccgggcccc ttgccatacc
     3001 acatcgagaa cacgccgtcg tattttgcgc cggggaacag gttgacctgc tgggtgcccc
     3061 agatgctggt ggccgcgagg tcttcgttca tgccgggatg gaacttcaca tggtgcgctt
     3121 cgaggtactt gcgtgccttc atcgcggtca tgtcgacgtt gcccagcggc gagccacgat
     3181 agccgctgat gaagccggcg gtgttgagcc cggcgcgcac gtcgcgctgg cgctgcatca
     3241 tcggcaggcg cacgagcgcc tgcgtgccgg tgatgaacgc gcggccgcgc tcgagggtga
     3301 atttgtcgtc cagggaaatg ttttccagca gcgcgcgctg cccggaagtc agaggtgcgt
     3361 tcaattcggt ctccagccaa aaattttgtc tttgggtgcg atgctttcgc tg
//
LOCUS       DATLGF010000318         5773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_46120,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000318 DATLGF010000000
VERSION     DATLGF010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46120"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VM406_09720"
     CDS             <1..492
                     /locus_tag="VM406_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVL76283.1"
                     /translation="SASVAENIAYARPDASAEEIERAARGAQAHDFIGRLPDGYDTRV
                     GERGLTLSGGQRQRIAIARALLKNPPLLLLDEATSALDAESERLVQKALEAAMVGRTT
                     LVIAHRLATVQSADRIVVMEHGRIVETGTHASLIASGGLYASLAALQFSQPSQSFDET
                     TVK"
     gene            516..1310
                     /gene="thyA"
                     /locus_tag="VM406_09725"
     CDS             516..1310
                     /gene="thyA"
                     /locus_tag="VM406_09725"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HVL76284.1"
                     /translation="MRQYLEFMRHVYEHGTEKTDRTGTGTRSVFGYQMRFDLQEGFPL
                     VTTKKLHLKSIIHELIWFLQGSTNIQYLKDNGVSIWDEWADGNGDLGPIYGYQWRNWP
                     TPDGRHIDQIAQVLEQIRNNPDSRRMIVSAWNVADIPQMKLPPCHAFFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTHMMAEQAGLEVGDFIWTGGDCHLYSNHMEQVQL
                     QLSRTPHPLPKLVIKRKPDSIFDYRYEDFEIEGYQSHPHIKAPVAV"
     gene            1322..1828
                     /locus_tag="VM406_09730"
     CDS             1322..1828
                     /locus_tag="VM406_09730"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HVL76285.1"
                     /translation="MTPHVPRLTIVVAMDRQRGIGVNNQLPWRLPEDLAHFKRTTMGH
                     PIIMGRKTFDSIGRALPNRRNIVITRNKTWKHEGVEVAHSLDAALALVGEVPACIIGG
                     AQIFEEALPQTGRLVVTHIDHDFPCKTFFPEIDPADWQEIARETHHSQANGFDFSYVT
                     YERKSPLA"
     gene            1913..3094
                     /locus_tag="VM406_09735"
     CDS             1913..3094
                     /locus_tag="VM406_09735"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVL76286.1"
                     /translation="MKHKTRTEHEARWTKWLGGAAVGALAMYFSDPERGRRRRALTRD
                     KMQSLVTQTGNAMDVARRDLGNRVQGLRARAFHLVKRDEHADDPVLAARVRARIGRAV
                     SHPHPIHVAAHDSCVTLTGPILASEHQQLIKAVRHVHGVREVEDMLTVHENAEGIPAL
                     QGNHRTRESAATAYWPPGLRALATAGGGFLALYGLRRRGAAGTLLGTAGLGLLARSLT
                     NRPLFPNGAASQSVHLTKTLHISASPERVYDMWSDYKHFPYFMSHVKEVRDLGDGRSH
                     WTVTGPAGMPIEWTARMTEQKPMEKLAWHTEEDAPVKHSGEVHFEPEMTGTRVTVHLS
                     YQPPAGAVGHVAASLLGASPKRQMDDDLMRMKSYIEQGIAPHDAAKPFPPLGEQVAGV
                     Q"
     gene            3323..5572
                     /locus_tag="VM406_09740"
     CDS             3323..5572
                     /locus_tag="VM406_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/lysine/ornithine decarboxylase"
                     /protein_id="HVL76287.1"
                     /translation="MKFRFPIVIIDEDFRSENTSGLGIRALAAAMEAEGMEVVGVTSY
                     GDLSQFAQQQSRASAFILSIDDEEFGGGSFEETDTALKALRAFVGEIRHKNADIPIYL
                     YGETRTSRHIPNDILRELHGFIHMFEDTPEFVARHIIREAKSYLDGLAPPFFRALVHY
                     AQDGSYSWHCPGHSGGVAFLKSPIGQMFHQFFGENMLRADVCNAVEELGQLLDHTGPV
                     AASERNAARIFNADHCYFVTNGTSTSNKMVWHSTVGPGDIVVVDRNCHKSILHSIIMT
                     GAIPVFLMPTRNHLGIIGPIPLDEFKMENIRKKIEANPFAREAKNKKPRILTITQSTY
                     DGVLYNVETIKQMLDGEIDTLHFDEAWLPHATFHDFYKNMHAIGKERPRAKESLIFST
                     QSTHKLLAGLSQASQILVRDSEKVKLDQDMFNEAYLMHTSTSPQYAIIASCDVAAAMM
                     EPPGGTALVEESILEALDFRRAMKKIDEEWGGDWWFKVWGPDVTSDQGIGTQEDWMIN
                     AEDEWHGFGKLAKGFNMLDPIKATIVTPGLSIEGQFADTGIPASIVTKYLAEHGVIVE
                     KAGLYSFFIMFTIGITKGRWNTLLTALQQFKDDYDKNQPMWRILPEFAAANPRYERVG
                     LRDLCHSIHYMYKAYDVARLTTEMYLSDMQPAMKPSDAFAKMAHREIERVSIEELEGR
                     VTAILLTPYPPGIPLLIPGERFNKTIVDYLRFARDFNEKFPGFETDVHGLVVQEIDGR
                     RGYYVDCVK"
     gene            5579..>5773
                     /locus_tag="VM406_09745"
     CDS             5579..>5773
                     /locus_tag="VM406_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011880588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HVL76288.1"
                     /translation="MDPDTFGRIVAATPLVSIDLVLTNERGQVLLGYRSNRPAQHCWF
                     VPGGRIRKDESLEEAWGRIAA"
BASE COUNT         1112 a         1968 c         1751 g          942 t
ORIGIN      
        1 agcgcctcgg tcgccgagaa catcgcctac gcgcggccgg acgcgagcgc ggaggagatc
       61 gagcgggcgg cccgcggggc gcaggcccac gacttcatcg ggcggctccc cgacggctac
      121 gacacccggg tcggcgagcg cggtctcacg ctgtcgggcg gccagcgcca gcgcatcgcg
      181 atcgcgcggg cgctgctgaa gaacccgccg ctgctgctgc tggacgaagc gaccagcgcg
      241 ctcgatgccg aatccgaacg gttggtgcag aaggcgctgg aagcggcgat ggtcgggcgc
      301 accacgctgg tcatcgcgca tcggctggcg accgtgcaga gcgccgaccg catcgtcgtg
      361 atggagcacg gccgcatcgt ggaaaccggc acgcatgcgt cgctgattgc ctcgggcgga
      421 ctgtacgcga gcctggcggc attgcagttt tcgcaaccga gccagtcgtt cgacgagaca
      481 acggtaaaat agcgcgcccc tatccgagat ttcccatgcg ccaatacctc gaattcatgc
      541 gccatgtgta cgaacatggc accgaaaaga ctgatcgcac cggcaccggc acgcgctcgg
      601 tattcggcta ccagatgcgc ttcgacctgc aggaaggctt tcccctggtg accaccaaga
      661 agctgcatct gaagtccatc atccacgagc tgatctggtt cctgcagggc tcgaccaata
      721 tccagtacct gaaggacaac ggcgtctcga tctgggatga atgggccgac gggaacggcg
      781 atctcggccc gatctacggc taccagtggc gcaactggcc gaccccggac ggccgccaca
      841 tcgaccagat cgcccaggtg ctggagcaga tcaggaacaa cccggattcg cgccgcatga
      901 tcgtctcggc ctggaacgtg gccgacattc cgcagatgaa gctgccgccc tgtcatgcgt
      961 tcttccagtt ctacgtcgcc gacggcaagc tctcctgcca gctctaccag cgcagcgccg
     1021 acatcttcct cggcgttccg ttcaacatcg cctcctacgc gttgctcacc cacatgatgg
     1081 ccgagcaggc cggccttgag gtcggcgatt tcatctggac cggcggcgac tgccacctgt
     1141 attcaaatca catggaacag gtgcagctgc agctttcacg cacgccgcac ccgctgccga
     1201 agctggtgat caagcgcaag ccggattcga tcttcgatta ccgctacgag gacttcgaga
     1261 tcgagggcta ccagagccat ccgcatatca aggcgccggt ggcggtttga acagaaaagc
     1321 catgactccc cacgtccccc gactcacgat cgttgtcgcg atggaccgcc agcgcggcat
     1381 cggcgtcaac aatcagctgc cttggcgcct gcccgaagac ctcgcgcatt tcaagcgcac
     1441 cacgatgggc catccgatca tcatgggccg caagaccttc gattcgatcg ggcgtgcgct
     1501 gccgaaccgc cgcaacatcg tcattacccg aaacaagacc tggaagcacg aaggcgtcga
     1561 ggtcgcgcat tcgctcgacg cggcgctcgc gctggtgggc gaggtgccgg cgtgcatcat
     1621 cggcggcgcg cagatcttcg aggaagcctt gccccagacc gggcgcctgg tcgtcacgca
     1681 catcgaccat gacttcccct gcaaaacctt cttcccggaa atcgatccag ccgattggca
     1741 ggaaatcgcc cgcgaaaccc atcactcgca agcaaacggt ttcgacttct cctacgtcac
     1801 ctacgagcgc aaatcgcccc ttgcctgaaa agcactgaac tcgcgcttgc ccggctcttc
     1861 caatacttca tttccgcctg actggcagtg ttggtgatga aaggagagca aaatgaagca
     1921 caagacacga acggagcacg aggcgcgctg gacgaaatgg ctcggcggcg ccgccgtggg
     1981 cgcgctggcc atgtacttct ccgatcccga acgcggccgg cgccgtcggg cgctgacgcg
     2041 ggacaagatg caaagccttg tgacccagac gggcaacgcg atggacgtgg cccggcgcga
     2101 cctgggcaac cgcgtgcagg gcctgcgcgc acgggccttt cacctcgtca agcgcgatga
     2161 acatgcggac gaccccgtgc tggcggcaag ggtgcgcgcg cgcatcggcc gcgcggtctc
     2221 gcatccgcat ccgatccacg ttgccgcgca cgacagttgc gtcacgctca ccgggcccat
     2281 cctggcctcc gagcatcagc aactgatcaa ggcggtgcgc catgttcatg gcgtgcgtga
     2341 agtcgaggac atgctcaccg tgcatgaaaa cgcagagggc attccggcac tgcagggcaa
     2401 ccaccgcacc cgtgagtcgg ccgccaccgc atactggccg ccggggctgc gcgcgctggc
     2461 caccgccggc ggcggcttcc tcgcgctgta cggcttgcgc cgccgcggtg ccgccggcac
     2521 gctgctgggc acggccggac tcggcctgct cgcgcgcagc ctcaccaacc gtccgctgtt
     2581 cccgaatggc gcggcaagcc aaagcgtgca cctaaccaag acgctgcaca tcagtgcctc
     2641 gccggaacgc gtgtacgaca tgtggagcga ctacaagcat ttcccctact tcatgtcgca
     2701 cgtgaaggag gtccgcgatc tcggcgacgg acgctcgcac tggaccgtga cggggccggc
     2761 cggcatgccg atcgaatgga ccgcgaggat gaccgagcag aaaccgatgg aaaaacttgc
     2821 atggcacacg gaggaggatg cgccggtcaa gcacagcggc gaggtgcatt tcgaacctga
     2881 aatgaccggc acccgcgtga ccgtgcacct gtcctaccag ccgccggccg gcgcggtcgg
     2941 ccatgtcgcg gcctcgctgc tcggggccag cccgaagcgc cagatggacg atgacctgat
     3001 gcggatgaag tcctatatcg agcagggcat tgcgccgcat gacgcagcca agcccttccc
     3061 tccgctgggc gagcaggtgg cgggcgtgca atagcccggc ggcccgcgcg gggggtgctg
     3121 cggggcgccc tcgctgcacc gctcgtccgt gtagccggcg gcgccccgca acccccgcgc
     3181 gagcgcatcg tttccggcct tacacaacgt ttttctccag caaacccccg ccatatctgc
     3241 gaaaattcgc cattcttttt tcggcgcgca agcactcccg cccaggcgtc tccgcattcc
     3301 atacgtttcc cctggagacc tcatgaaatt ccgcttcccg atcgtcatca tcgacgaaga
     3361 cttccgctcc gaaaacacct ccggcctcgg catccgcgcg ctcgccgccg ccatggaggc
     3421 cgagggcatg gaagtggtcg gcgtgaccag ctatggcgac ctgtcgcagt tcgcgcagca
     3481 gcagtcgcgc gcctccgcct tcatcctttc catcgatgac gaggagttcg gcggcggctc
     3541 gttcgaggaa acggatacgg ccctgaaggc gctgcgcgcc ttcgtcggcg agatccgcca
     3601 caagaacgcc gacatcccga tctacctgta cggcgaaacc cgcacctcgc gccatatccc
     3661 gaacgacatc ctgcgcgagc tgcacggctt catccacatg ttcgaggaca cgccggaatt
     3721 cgtcgcgcgc catatcatcc gcgaggccaa gtcctatctc gacggcctgg cgccgccgtt
     3781 cttccgcgcg ctggtgcact acgcgcagga cggttcctat tcgtggcact gccccggcca
     3841 ctccggcggc gtggctttcc tgaagtcgcc gatcggccag atgttccacc agttcttcgg
     3901 cgagaacatg ctgcgtgccg acgtctgcaa cgccgtggaa gaactcggcc agctgctcga
     3961 ccataccggc ccggttgcgg cgtcagagcg caatgcggcg cgtatcttca atgccgacca
     4021 ctgctacttc gtcaccaacg gcacctcgac ttcgaacaag atggtctggc actcgacggt
     4081 cggccccggc gacatcgtcg tggtcgaccg caactgccac aagtccatcc tgcactcgat
     4141 catcatgacc ggcgcgattc ccgtcttcct gatgccgacc cgcaatcacc tcggcatcat
     4201 cggtccgatt ccgctcgacg agttcaagat ggagaacatc cggaagaaga tcgaggccaa
     4261 tccgttcgca cgcgaggcga agaacaagaa gccgcgcatc ctgacgatca cgcagtcaac
     4321 ctacgacggc gtgctgtaca acgtggaaac gatcaagcag atgctggacg gcgagatcga
     4381 tacgctgcac ttcgacgaag cctggctgcc gcacgcgacc ttccatgact tctacaagaa
     4441 catgcacgcg atcggcaagg agcgtccgcg cgcaaaggaa tcgctgatct tctccacgca
     4501 gtcgacccac aagctgctgg ccggcctgtc gcaggcctcg cagatcctgg tgcgcgattc
     4561 cgagaaggtg aagctcgacc aggacatgtt caacgaggcc tacctgatgc acacctcgac
     4621 ttcgccgcag tacgcgatca tcgcctcctg cgacgtcgct gccgcgatga tggagccgcc
     4681 gggcggcact gcgctggtgg aggaaagcat cctggaagcg ctcgacttcc gccgcgcgat
     4741 gaagaagatc gacgaggaat ggggcggcga ctggtggttc aaggtctggg gtcccgacgt
     4801 gacatcggac cagggcatcg gcacccagga agactggatg atcaacgccg aggacgaatg
     4861 gcatggtttc ggcaagctcg ccaagggctt caacatgctc gacccgatca aggcgaccat
     4921 cgtcacgccc ggcctgtcga tcgagggcca gttcgccgac accggcatcc ccgcgtcgat
     4981 cgtgaccaag tacctggccg agcatggcgt catcgtcgag aaggcgggcc tgtactcgtt
     5041 cttcatcatg ttcacgatcg gcatcaccaa gggccgctgg aacaccctgc tgaccgcgct
     5101 gcagcagttc aaggacgact acgacaagaa ccagccgatg tggcgcatcc tgcccgagtt
     5161 cgccgcggcg aacccgcgct acgagcgggt cggcctgcgc gacctttgcc acagcatcca
     5221 ctacatgtac aaggcctacg acgtggcgcg cctgacgacg gagatgtatt tgtccgacat
     5281 gcagccggcg atgaagccct cggacgcctt cgcgaagatg gcgcaccgcg agatcgagcg
     5341 cgtctcgatc gaagaactgg aaggccgcgt gaccgcaatc ctgctgacgc cctacccgcc
     5401 cggcatcccg ctgctgatcc cgggcgagcg cttcaacaag accatcgtcg actacctgcg
     5461 tttcgcgcgc gacttcaacg agaagtttcc cggcttcgag accgacgtgc acggcctggt
     5521 ggtgcaggaa atcgacggcc ggcgcggcta ctacgtcgac tgcgtgaaat agccccgcat
     5581 ggacccggat acgttcggcc gcatcgtcgc cgccacgccg ctggtgtcga tcgacctggt
     5641 gctgaccaac gaacgcggcc aggtcctgct gggctatcgc agcaaccggc cggcccagca
     5701 ttgctggttc gtgccgggcg gccgtatccg caaggacgaa agcctggaag aggcctgggg
     5761 ccgcattgcc gcc
//
LOCUS       DATLGF010000319         9669 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_6877,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000319 DATLGF010000000
VERSION     DATLGF010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 9669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9669
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6877"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VM406_09750"
     CDS             <1..530
                     /locus_tag="VM406_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HVL76289.1"
                     /translation="GLYAVGETAYTGLHGANRLASNSLLECVVIGRAAASHIEAQPRQ
                     QAMPLPAWDESRVSDADEEVVIAHNWDELRRFMWSYVGIVRTTKRLERAQHRIRLLRD
                     EIYEYYANFRITRDLLELRNLVDVASLIVESALLRRESRGLHFSQDYPETLPKALPTV
                     LAPRKRQPAREHSRR"
     gene            705..1571
                     /locus_tag="VM406_09755"
     CDS             705..1571
                     /locus_tag="VM406_09755"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVL76290.1"
                     /translation="MATIQHSIQVKVPVQTAYNQLTQFEEFPRFMHSVDAVEQIDDNH
                     LHWRTKMADQPVEWDAEIVEQEPDRCIAWRSTSGPRETARLDVLPLGQDGAEVTFTID
                     VALEELQESIAGRAQEEMSRRLREDMERMREYIEAHGRETAGWRGEIHDAKVTLRDRD
                     AKGRSGAAAGGQGQRVQNEPAPMGGHAPTAAVVGAAGGTDASAGAKMSGSKGPDAPGL
                     RESTTAEGAAGSAVKGDAVTPGAGSPGGTGLGAAGSAEDTRTGTGGTSGSASSLTGGG
                     MSGARDAGKGKA"
     gene            1663..2262
                     /locus_tag="VM406_09760"
     CDS             1663..2262
                     /locus_tag="VM406_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HVL76291.1"
                     /translation="MDLALNGMNIAILVTDGFEQVELTEPKKALEAEGAFTRIVSDRY
                     DTVRGMHHEQPGEEFQVDLRLEEADAKAFDALLLPGGKVNAAALRNIEEAQRFARAFD
                     DDGKPIAVICHGAWLLVSAGLVEGRTLTSHPTLEQEIRDAGGHWVDQEVVVDGMLVSS
                     RKPADIPAFNDKTIEVIAARMQANLQGKADGHAVGIATS"
     gene            2288..3139
                     /locus_tag="VM406_09765"
     CDS             2288..3139
                     /locus_tag="VM406_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVL76292.1"
                     /translation="MQTVSQLMTRNVQVVSPHETIRRAAQMMDELNVGALPVCDGERL
                     VGMITDRDITVRATSAGMAPDEAHVDEVMSADVRWCYEDQPLDEVMQQMADTQIRRVP
                     VVSHDDARRLLGIVSLGDIATKANSGNKYQDAGQLMAGVSSPSEPDRSGAHRPAPPAG
                     RMTSDVATGAGLAGSDVLDAGMNRHGASSTGDPSDVVEVGPDDLVNRNAPDAKAVNVE
                     QPGSARRVSETEKNGVKVRRTVADGSDTSTGESGAPAGIDSGAAGASGGGAGTSGSPG
                     TGIGAKP"
     gene            complement(3164..3772)
                     /gene="gstA"
                     /locus_tag="VM406_09770"
     CDS             complement(3164..3772)
                     /gene="gstA"
                     /locus_tag="VM406_09770"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HVL76293.1"
                     /translation="MKLYYSPGACSLAPHIVALETGQPLELVKVDIPNKKTENGDDYW
                     QVNPKGYVPALVLDNGEVLTEVAVICQYLADQAPDSGMIAPFGSAERYRQMAALNFAA
                     TEVHKQIGALFNPALTPEMRTVQTATISRRLNALEKMLDGCPYIMGERFTAPDAYLFT
                     VLNWARVHKIDLAPWPNIRAYMARVAERDKVQAAMRAEGLVK"
     gene            3925..4737
                     /locus_tag="VM406_09775"
     CDS             3925..4737
                     /locus_tag="VM406_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76294.1"
                     /translation="MSITINETLRAYIDPLTEDEYAALERSLLTEGCRDALVLWGDVL
                     VDGHNRYGICQKHGIPFKTVQNETFKSMDDVYLWMIDNHLGRRSVSDFQRGVLALRKK
                     EILAARQAQEKAQPEPEQAPANEETVAKPAEPPLTREVLARAARVSSATLGQIEKIQK
                     TAAPELVDAVRAGVISINAAAAVASLPEEKQKEAVAGGRNELKLAARQVREARLPPRP
                     EPAPPPEDATPEQIEIHRLRQKVAELTDERDQLKKKVQHLTIALAEARGGAQ"
     gene            complement(4903..5853)
                     /locus_tag="VM406_09780"
     CDS             complement(4903..5853)
                     /locus_tag="VM406_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVL76295.1"
                     /translation="MDARKPLDAQAVGLMLFLCLCWALQQVALKATAADIAPILQISL
                     RSGIAALLVGALMLAQREKLSLAGGEWRPGMVVGALFALEYLLLGEALRLTSASHAAV
                     FLYTAPIFAALGLHWKLPSERMSALQWLGIALAFCGIALAFLGRDTQAPTVAVSSMLL
                     GDLLALLAGAAWGATTIVIRTTSLSVLPAKQTLLYQLVGAFVLLLACAVAFGQTGFKP
                     TPLALASLGFQAVIVSFFTFLLWFWMLRHYLASRLGTLSFMTPLFGVVLGAWLLKEPI
                     EPGFLAGAVLVMAGIVLVSGHGWLGQLAGAIPRLLKRRSA"
     gene            5954..6751
                     /locus_tag="VM406_09785"
     CDS             5954..6751
                     /locus_tag="VM406_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVL76296.1"
                     /translation="MKSLTRHLHPPLFSEAFRTPIVFRAASIPADGAYPVHRHTWGEF
                     VYSFSGVIEVRIGARQYLAPPQYGIWLPPDVEHQGLNRYEACHSSLYVAATLCEGLPH
                     EACALTVSPLVRAMLEHLREHPPQWPHSAQDGRLLQVVVDQLGSAARAGSYLPTTEDP
                     LLGAVLAMLEADPGDTRSVAQLAQAVHTTERTLVRRCQRELGMSLSEWRQRLRVVKAM
                     PLLEAGEKIEAIALDLGYGSASAFIAMFRRLMGVTPDEYRRQAQGTV"
     gene            7012..8199
                     /locus_tag="VM406_09790"
     CDS             7012..8199
                     /locus_tag="VM406_09790"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVL76297.1"
                     /translation="MQSGYQAGELMLERVRRLGSEWMASMDAQTAIRLKSTGSAAVDY
                     TLFILIMAGFAATGSVPASAVWILLAVALALNALFIGSQWSGLTRRLKDPSITWPQVI
                     GACGINFLAMFLAPKVLPVFIVNLFIPLCYGGLYFSRREFLAAWALLALSLGATMLTL
                     GMRPSGLGMLDWSPAEQLLLWVAVAAALGRFLFINAEVSRLRERLHRKNKELLVATTR
                     LAELASHDELTGLLNRREFIRLLDEEHHRSERGHGGFAVAIVDADHFKSINDRFGHLV
                     GDRVLKELARLLGARCRSSEALARYGGEEFTLLLHDRSAHAVTQALERLRDGVARHPW
                     DKIAPGLRVTISAGAAIPFNGETVQQLLSRADVALYEAKAAGRNRVRLSEPAPSPAAV
                     QAD"
     gene            8334..>9669
                     /locus_tag="VM406_09795"
     CDS             8334..>9669
                     /locus_tag="VM406_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HVL76298.1"
                     /translation="MSFVEQRARPVGTMQAVLKEYMDPDSGARHIHLATDDPEMVFLV
                     AFPTVPDKSDGRAHILEHLALCGSQRYPVRDPFFAMLRRSTSTFMNAMTYPDRTVYPF
                     ASTDPADFNNLLGVYLDAAFFPKLDYLDFLQEGWRYTLENGKLGYGGVVFNEMKGAFG
                     DPMRALMGDLSAIMLKGTTYEVESGGDPLEIPSLTHEELKEFHASHYHPSQAIFMTAG
                     RLDPVAVQSVIAEQVLSRLPGKAPRRMPELAAHWDAPKEATILVPSPTARDDEFGVQF
                     AWLLGESSDPLAYCRAHLLESGLIGDASSPVMRAMESAGYGRPSELNGFDASYRQMLF
                     HLGMEGLTREETAAAKTLIRDALESAAEKGVPQSALEAALRDFRFSQREVRGGGVPHG
                     LRKLLHALPLEMAGADLLPAFDNEATLARLDDAVRDPAFFKSLVRELLAAPTR"
BASE COUNT         1736 a         3170 c         3258 g         1505 t
ORIGIN      
        1 ccggcctgta tgcggtcggc gagaccgcat acaccggttt gcacggcgcg aaccggcttg
       61 ccagcaattc gctgctcgaa tgcgtggtga tcggccgcgc ggcagcaagc cacatcgaag
      121 cccagccccg gcagcaggcg atgccgttgc cggcctggga cgaaagccgc gtcagcgacg
      181 ccgacgagga ggtcgtgatc gcgcacaact gggacgaact gcgccgcttc atgtggagct
      241 atgtcggcat cgtgcgcacg accaagcgcc tggagcgcgc ccagcaccgc atccgcttgc
      301 tgcgtgacga gatctatgag tactacgcga atttccgcat cacccgcgac ctgctggaac
      361 tgcgcaatct cgtcgacgtc gcctcgctga tcgtcgagag tgcgctgctg cggcgcgaga
      421 gccggggtct gcatttcagc caggactatc cggagactct gccgaaggct ctgccgaccg
      481 tgctcgcgcc gcgcaagcgg cagcctgcgc gcgaacacag ccggcgctag gccgttttac
      541 ccccgccggc ccgctgggcc ggccctccat cgctccctcc tcgcgcaaac ggcgcaactt
      601 gtcgcgccat gcgcactctg actcgtattg cttaaagcat catgagccgc acatgacagc
      661 ggttcccgct acacacaatc gcgagtcaga aaaggagatt caacgtggca acaattcagc
      721 actcgatcca ggtcaaggtg ccggttcaga cggcctacaa ccagctcacg cagttcgagg
      781 aatttccgcg cttcatgcac agcgtggacg cggttgaaca gatcgatgac aaccacttgc
      841 attggcgcac gaaaatggcg gaccagccgg tcgaatggga cgcggagatc gtcgagcagg
      901 agcccgaccg ctgcatcgcg tggcgcagca ccagcggacc gcgcgagacc gcccggctcg
      961 acgtgctgcc gctcggccag gatggtgctg aagtcacgtt tacgatcgat gtcgcgctgg
     1021 aggaattgca agaatccatt gccggccggg cccaggaaga gatgagtcgc cggctcaggg
     1081 aagatatgga gaggatgcgc gaatacatcg aagcccacgg aagggaaacc gcgggctggc
     1141 gcggcgagat ccacgacgcg aaggtgacgt tgcgggaccg cgacgcgaag ggccgctccg
     1201 gcgcggcggc aggcgggcaa ggccagcgcg tgcagaacga gccggcgcca atgggtggac
     1261 atgcgcccac tgcggccgtg gtgggagcgg caggcggcac cgacgcctcg gcaggcgcga
     1321 agatgtccgg cagcaagggg ccggacgcgc cgggcctgag ggaatccacc accgccgagg
     1381 gtgcggccgg cagcgcagtc aagggtgacg cggtgacgcc cggggccggc tcgccaggtg
     1441 gcaccggcct gggggccgcc ggcagcgcgg aagacacgcg cacaggcacc ggcggcactt
     1501 cgggatcggc gtcctcactc actggcggcg gcatgagcgg cgcgcgggat gccgggaaag
     1561 gaaaagcctg aaggggcagg cgttgcgcgg ttgtccgtta gcgcgcgccg aacttttcca
     1621 atgccgttca atcaacaggg ttttgccaca tggagaaacg ccttggacct ggcactgaac
     1681 ggcatgaata tcgccatcct ggttacggat ggttttgagc aggtggaact gacggagccg
     1741 aagaaggcgc ttgaggcgga gggtgcgttt acgcgcatcg tctccgacag gtacgacacg
     1801 gtgcgcggca tgcaccacga gcagccgggc gaagagttcc aggtcgacct gcggctcgag
     1861 gaggccgatg ccaaggcctt cgacgcgctg ctgctgcccg gcggcaaagt gaatgcggcg
     1921 gccttgcgca acatcgagga agcgcagcgc ttcgcccggg ccttcgacga cgacggcaag
     1981 ccgatcgcgg tgatctgcca tggcgcctgg ctgctcgtct ccgcgggact ggtcgaggga
     2041 cgcacgctca ccagtcatcc gacgctggaa caggagatac gcgacgccgg cggccactgg
     2101 gtcgaccagg aagtggtggt cgacggcatg ctcgtcagca gccgcaagcc cgccgacatc
     2161 cccgccttca acgacaagac gatcgaagtc atcgccgcgc gcatgcaggc caatctgcag
     2221 ggcaaggccg acggccacgc ggtcggaatt gccaccagct gagcgtgcgc aacgaaagga
     2281 gaatgcgatg cagacggtct cgcaactgat gacacgcaat gtgcaggtcg tttccccgca
     2341 cgagacgata cggcgcgctg cgcagatgat ggatgagctg aacgtcgggg cgctgccggt
     2401 atgcgacggc gagcggctcg tgggcatgat caccgaccgc gacatcacgg tgcgcgccac
     2461 ctcggccggc atggcgccgg acgaggcgca tgtcgacgaa gtgatgagcg ccgacgtgcg
     2521 ctggtgctat gaagaccagc cgctagacga ggtgatgcag cagatggccg atacccagat
     2581 ccgccgcgtg ccggtcgtct cgcacgacga tgcgcgcagg ctgctcggca tcgtgtcgct
     2641 cggcgatatc gcgaccaagg cgaacagcgg caacaagtat caggacgcgg gccaactgat
     2701 ggccggcgtc tcctcgccgt cggaaccgga tcgctccggg gcgcaccgcc ctgccccgcc
     2761 ggccggccgc atgacttcgg atgtggccac cggcgcggga cttgctggat cggatgtgct
     2821 cgacgccggc atgaacaggc acggcgcgtc gtcgaccggt gatccgagcg acgtggtcga
     2881 ggtcggcccc gatgatctgg tgaacaggaa cgcgccggat gcgaaggcgg tgaacgtcga
     2941 acagccgggt tccgcccgac gcgtgtcgga aacggagaag aacggcgtga aggtgaggcg
     3001 cacggttgcc gacggctcgg atacgagcac cggagaaagc ggcgctccgg ccggcatcga
     3061 cagcggcgcc gccggcgcat ccggcggcgg cgcgggaacg tcgggctcgc cggggacagg
     3121 gatcggggcc aagccctgat tcaacatgcc gccccggcgc ggttcacttg accaggccct
     3181 cggcccgcat cgcagcctgc actttgtccc gctcggcgac gcgtgccatg taggcacgga
     3241 tgttcggcca gggcgccaga tcgatcttgt gaacacgcgc ccagttcagc accgtgaaca
     3301 ggtaggcgtc cggcgctgtg aagcgctcgc ccatgatgta ggggcacccg tcgagcatct
     3361 tttcgagcgc gttgaggcgg cgcgagatcg tcgcagtctg caccgtgcgc atctccggcg
     3421 tcagggccgg attgaacagc gcgccgatct gcttgtgcac ttcggtagcg gcgaagttca
     3481 gcgccgccat ctgccggtag cgctcggcgc tgccgaacgg cgcgatcatt ccgctgtcgg
     3541 gcgcctggtc ggccaggtac tggcagatca ccgccacctc ggtgaggacc tcgccgttgt
     3601 ctagcacgag ggcaggcaca tagcctttcg gattgacctg ccagtaatcg tcgccgttct
     3661 cggtcttctt gttcggaatg tcgaccttga ccagttccag cggctgcccg gtttcgagcg
     3721 cgacgatatg cggcgccagc gaacaggctc cgggcgagta gtacagtttc atggacttcc
     3781 ctttcgatgc gaacgccacc ttctgtggca ggcattcatt ctgccgcaac cggcgctggc
     3841 gcgtattcca ccgcccggcc gctgcgaccg cgcatcctgg cgttcgcgtg taaactgccg
     3901 ccctcgcctt gcccgaagcc cgttatgtcc atcacgatca acgaaaccct gcgcgcctat
     3961 atcgacccgt tgacggagga cgagtacgcg gcgctggaac gcagtctgct caccgaggga
     4021 tgccgcgacg cgctggtgct gtggggcgac gtgctggtgg acggccacaa ccgctacggc
     4081 atctgccaga agcacggcat cccgttcaag accgtgcaga acgagacctt caagtcgatg
     4141 gacgacgtct atctctggat gatcgacaac cacctgggac ggcgcagcgt gtcggatttc
     4201 cagcgcggcg tgctcgcgct gcgcaagaag gagatcctgg ccgcgcgaca agcccaggaa
     4261 aaagcgcagc cagaacctga acaggcgccg gcaaatgagg aaaccgtagc gaagcctgcc
     4321 gagccgccct tgacgcgcga agtgctggcc cgtgctgcgc gcgtcagcag cgcgacgctc
     4381 ggccagatcg agaagattca gaaaaccgcc gcgcccgaac tcgtcgatgc ggtcagggcc
     4441 ggcgtgattt cgatcaacgc ggcggcggcg gtcgcttcgc ttccggagga aaagcagaaa
     4501 gaggcggtcg ccggcggcag gaacgaattg aagctggcgg cgcgccaggt gcgcgaggcg
     4561 cggctgccgc cgaggccgga acccgcgccg ccgcccgagg atgcgacgcc cgagcagatc
     4621 gagatccacc gcctcaggca gaaggtcgcg gaactcaccg acgagcgcga ccagttgaag
     4681 aagaaggtgc agcacctgac gatcgcactg gccgaggcgc gcggcggcgc gcagtaggcg
     4741 ttaaggctac gctggataag tcccgcgagg gcgcgcgccc gaagtcggga tgggctgcaa
     4801 ggcgcacacc gcagcgatag cggggctatc gcgaggatgg gcaacgccgc agaccgcccg
     4861 agttcgggat gcgcgacgcg cggggacttg ttcagcgtag ccttaggccg agcggcgttt
     4921 cagcagccgc ggaatcgcgc cggccagctg gcccagccat ccgtgcccgc tcaccagcac
     4981 gatgccggcc atgaccagca ccgccccggc gagaaagccg ggttcgatcg gctccttcag
     5041 cagccaggcg ccgagcacca cgccgaacag cggtgtcatg aacgacagcg ttcccagccg
     5101 cgaggccaga tagtggcgca gcatccagaa ccacagcagg aaggtgaaga aggatacgat
     5161 caccgcctgg aagccgaggc tggccagcgc cagcggggtc ggcttgaatc cggtctggcc
     5221 gaacgcgacc gcgcaggcca gcagcagcac gaaggcgccg acgagctgat acagcagggt
     5281 ctgcttggcg ggcaggacgg acaggctggt ggtgcgaatc acgatagtgg tcgcgcccca
     5341 tgcggcgccg gcaagcagcg cgagcaagtc gcccagcagc atgctggaga ccgcgaccgt
     5401 cggcgcctgc gtgtcccggc cgaggaaggc cagcgcgatg ccgcagaacg cgagcgcgat
     5461 ccccagccac tggagcgcgg acatccgctc cgacggcagc ttccagtgca ggccgagcgc
     5521 ggcgaagatc ggcgcggtat agaggaagac ggcggcgtgc gaggcggagg tcagtctcag
     5581 cgcttcgccg agcagcaggt attcgagcgc gaacagcgca ccaaccacca tccctggccg
     5641 ccattcgccg ccggcgagcg agagtttctc gcgttgcgcc agcatcagcg cgccgaccag
     5701 caaggccgcg atgccggagc gcagcgagat ctgcaggata ggggcgatgt cggccgcggt
     5761 tgccttgagt gcaacctgct gcagggccca gcacaagcag aggaagagca tcaggcctac
     5821 ggcctgcgcg tccagcggtt ttctcgcgtc catgcaatcg gggaaaggca gttcgaacac
     5881 gcattgtccg gctgtcggct acgatccata tagttagaat cggacatccg atagcgccct
     5941 cccgccacac cccgtgaaaa gcctgacccg tcacctgcat ccgccactgt tcagcgaggc
     6001 cttccgcacg cccatcgttt tccgcgcggc aagcatcccg gccgatggcg cctatccggt
     6061 gcatcgtcac acctggggcg agttcgtcta ttcgttcagc ggcgtgatcg aagtcaggat
     6121 cggggcgcgg cagtacctcg ctccgccgca atacggcatc tggctgccgc cggacgtgga
     6181 gcaccagggc ctgaaccgct acgaagcctg tcacagctcg ctctatgtgg ccgcgacgct
     6241 gtgcgaaggc ttgccgcacg aagcctgcgc gctgaccgtt tcgccgctgg tgcgggcgat
     6301 gctcgagcac ctgcgcgagc atccgccgca gtggccccat tccgcgcagg acggccggct
     6361 cctgcaggtg gtggttgatc agctcggcag cgccgcccgc gcgggcagct atctgccgac
     6421 caccgaggat ccgctgcttg gtgcggtgct cgcgatgctg gaggccgatc cgggcgacac
     6481 gcgttcggtc gcgcagctcg cacaggccgt gcacacgacc gagcgcacgc tggtgcggcg
     6541 ctgccagcgc gagctcggca tgtcgctctc cgagtggcgt cagcgcctgc gcgtggtcaa
     6601 ggcgatgccg ctgctggaag ccggggaaaa gatcgaggcg attgcactgg acctgggtta
     6661 tggcagtgca tcggcgttca tcgcgatgtt ccgtcggctg atgggcgtca cgccagacga
     6721 ataccgcagg caggcgcaag ggacggtgtg agccggaacg ccgtttattt gagcgaagaa
     6781 ccttgcaatg tcgctcccgc gcaggcggaa gcccagctca ggtgcagcag tactacagac
     6841 gtctgaactg ggttcccgcc tgcacgggaa cgacatagca agagtagtcg tttgactgaa
     6901 ataccttccc attcacgccg ttccctgcgc ccgcctgcca gcttgtgccg ctctcgcaac
     6961 gctccccgtg ccgccgcgga tgccaaaagt tgccgaactg gaatatgctc gatgcaatcc
     7021 ggttaccaag cgggggaatt gatgctcgaa cgtgtgcgcc gtctcggcag cgagtggatg
     7081 gcatccatgg acgcccagac cgcgatccgc ctgaaatcga ccggctcggc cgcggtcgac
     7141 tacaccttgt tcatcctcat catggccggt tttgccgcta ccggctccgt ccctgcttct
     7201 gccgtatgga ttctgctggc agtcgcgctc gcccttaatg cgctgttcat cggtagccaa
     7261 tggtccggcc tgacgcgccg cctgaaggac ccgtcgatca cctggccgca ggtcatcggc
     7321 gcctgcggaa tcaatttcct cgcgatgttc ctcgcgccca aggtgctgcc ggtgttcatc
     7381 gtcaatctgt tcattccgct ttgctacggc ggactttact tcagccggcg cgagttcctg
     7441 gcagcctggg cactgctggc gctgtcgctt ggcgcgacca tgctcacgct gggcatgcgc
     7501 ccgtccggtc tcggcatgct ggactggagt ccggccgagc aactgctgct ctgggtcgcg
     7561 gtggcggccg cgctagggcg ttttctgttc attaacgcgg aagtctcgcg cctgcgggag
     7621 cgcctgcatc ggaaaaacaa ggagctgctc gtggcaacca cccggcttgc cgagctggcg
     7681 agccacgacg aattgacggg cctgttgaac cggcgcgagt tcatccgcct gctggacgag
     7741 gaacaccatc gcagcgagcg cgggcacgga ggctttgcgg tagccatcgt cgatgccgac
     7801 catttcaaga gtatcaacga tcgtttcggt catctggtcg gcgaccgggt gctgaaggaa
     7861 cttgcgcgtt tgctgggcgc gcgatgccga tcatcggaag cactggcgcg ctatggcggc
     7921 gaggaattca cgctgctgct gcatgaccgc agcgcgcacg ccgtcacgca ggcgctggag
     7981 cggttgcgcg acggggttgc gcgtcacccc tgggacaaga tcgcccccgg cctgcgcgtg
     8041 acgatttcgg ccggcgccgc cattcctttc aacggggaaa cggtgcagca actgctgtcg
     8101 agggccgacg ttgccctgta cgaagcgaag gccgccggcc gcaaccgcgt caggctgtcc
     8161 gaaccggcac cgtcgcctgc cgcggtgcag gccgactgat accccttcgc cggcgcccgc
     8221 ccgctccgcc ggctcgatcc gcaacgccag tcgcatgcgc gattcaaagc gcgcacgatc
     8281 ccgccccagt acaatcgaac gtccattcgc cgaaccttag ataggaatcc ccgatgagtt
     8341 ttgtggagca gcgcgcccgc ccggttggca ccatgcaggc tgtcctgaag gaatacatgg
     8401 atccggatag cggcgcacgc catatccatc tcgcaaccga cgacccggag atggtgtttc
     8461 tggtcgcgtt cccgaccgtg cccgacaaga gcgacggccg cgcgcacatc ctcgagcacc
     8521 tggccctgtg cggatcgcag cgctatccgg tgcgcgaccc gttcttcgcg atgctgcggc
     8581 gctcgacctc gaccttcatg aacgcgatga cctatccgga ccgcaccgtc tatccgtttg
     8641 ccagcaccga tccggccgat ttcaacaacc ttctcggcgt ttacctggac gcggccttct
     8701 tcccgaagct ggactacctc gacttcctgc aggaaggctg gcgctacacg ctggagaacg
     8761 gcaagctcgg ctacggcggc gtggtgttca acgagatgaa gggcgcgttc ggcgacccga
     8821 tgcgcgcgct gatgggcgat ctgtccgcga tcatgctcaa gggcacgacc tacgaggtcg
     8881 agtccggcgg cgatccgctc gagatcccgt cgctgaccca cgaggagctg aaggaattcc
     8941 acgccagcca ctatcacccg tcgcaggcga tcttcatgac ggccggccga ctggatccgg
     9001 tcgccgtgca gagcgtgatc gccgagcagg tgctgtcgcg cctgcccggc aaggcgccgc
     9061 gccgcatgcc ggaactggcc gcgcactggg atgcgccgaa ggaagcgacc atcctcgtgc
     9121 cctccccgac cgcgcgcgat gacgagttcg gcgtgcagtt cgcctggctg ctcggtgaat
     9181 cctcggaccc gctcgcgtat tgccgcgctc atctgctcga aagcgggctg atcggcgatg
     9241 cctcgtcgcc ggtgatgcgc gcgatggaat cggccggcta cggtcggccc tcggagctga
     9301 acggcttcga cgccagctac cggcagatgc tgttccatct cggcatggag ggactcacgc
     9361 gcgaggaaac cgcggcggca aagacgctga tccgggatgc gctggaatcc gctgccgaaa
     9421 aaggcgtgcc gcagtccgcg ctggaggccg cgctgcgcga cttccgtttc tcgcagcgcg
     9481 aggtcagggg cggcggcgtg ccgcacggcc tgcgcaagct gctgcacgcg ctgccgctgg
     9541 agatggccgg cgccgacctg ctgccggcct tcgacaacga agccacgctc gcccgcctcg
     9601 acgacgcggt gcgcgatccg gcgttcttca agtcgctggt gcgtgaactg ctggccgcgc
     9661 cgacccggc
//
LOCUS       DATLGF010000320         4123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_6883,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000320 DATLGF010000000
VERSION     DATLGF010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6883"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VM406_09800"
     CDS             <1..350
                     /locus_tag="VM406_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HVL76299.1"
                     /translation="WLGGYGTLLGAIAGVLLADYYLVRRQKLVVQDLYLRGGSYEYSG
                     GWNLMAVLALVLGVAPCLPGYLVVSGVLDQGAVAPFWVTLFDFGWFVSLAIAGAVTLL
                     ARPRGVTLARAVG"
     gene            complement(420..2234)
                     /locus_tag="VM406_09805"
     CDS             complement(420..2234)
                     /locus_tag="VM406_09805"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HVL76300.1"
                     /translation="MDDMLKEFVVEALDLATNVEEHLLSLERRPDDSDSLNALFRSFH
                     TIKGGAGFMNLTAIVSACHLTENLFDALRTGKVPVTPSAIEAGLQASGFVGDQLKQLA
                     NGASPDSLPSMPPELEVLLTEAIEGKSAPARAIEPAPSVQPPPAIDAGAGSADGPDWE
                     ALYRAVVPASAAVPAAESAAQAAPAVPSTGAAQPKAAADEARPITTQVKEDTIRIDAI
                     KLDALLEVAGESVQAANQAAVLLEKLAQFKFEGQAAALMATLQETLSRASRYSSELQR
                     ATLSTRMQPVGRLFQKFPRLVRELARDLGKDVDLVIEGAETEVDRVVVDSLYDPLVHM
                     LRNALDHGIETAEERAATAKPAKATILLKAWQEGSSVMIEVSDDGKGMDADRLRAKAL
                     AKGLIGEHQAQTAEEAFQLVFLPGFSTKEVASSVSGRGVGMDVVRTAVEKHRGAIRID
                     SHLGEGTTFTIRLPIELSIVPTMLVRTSGALLAIPMAVVQRVVELPESFDEVGGAPVL
                     RDQGRPLAVRSLAEILGYEPCREKVGIVIAAPHPYIVAVEGVDGTADLVIKPLTAIAV
                     PGVNGTARSAEGELVLVISLAFLLDGCKHSSPGTLLAA"
     gene            complement(2234..2998)
                     /locus_tag="VM406_09810"
     CDS             complement(2234..2998)
                     /locus_tag="VM406_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HVL76301.1"
                     /translation="MSDAADDDLEALFDQISAGRGTTAAEAPPAPPAEAAANNPDLAS
                     TALPAAPEAHDHDNSMYERLGGILRMLHDSLRELGFDRSLSDVASQITDAQSRLEHIA
                     SLTEQAANKVLNTVDTSLPEQEALSKQAQEMDERWTQLFAGKLSIEEFKSLAGDSRQF
                     AAHVMNASEAEKARLLDIMMAQDFQDITGQLIKKIVSITSTAERELAQLLRDNAPPEV
                     RAAMEQKPAELMQGPSAPGQAMAQDDVDSLLADLGF"
     gene            complement(3167..4015)
                     /locus_tag="VM406_09815"
     CDS             complement(3167..4015)
                     /locus_tag="VM406_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL76302.1"
                     /translation="MSKPDPDFYNRQYNARATIPDHPRIFTRWQKDSAAVRRQLAGLF
                     DIAYGDASGERLDFFPASRGNAPLLVFIHGGWWRSLDKSDFSFVVPAYTRAGFNVALT
                     NYTLAPQASVAEIVAEQLRALAWLYRHAEQYDFDAGRIVVAGHSAGAHLAAMMMAAHW
                     PVYADDLPADLVKGAVLMSGIYDLEPVRHADFVNVDLKIGEADVARLSPALMPPHRPI
                     PFVTACGARESDEFRRQTALIENAWKDAHQTTVPLPQSNHLTICDAFADPDHALCRAS
                     LALLAR"
BASE COUNT          597 a         1321 c         1474 g          731 t
ORIGIN      
        1 cctggctagg cggctacggc acgctgctgg gcgcgatcgc cggtgtgctg ctggccgatt
       61 actacctggt gcggcggcag aagctggtcg tgcaggatct gtatctgcgc ggcgggtcgt
      121 acgagtacag cggcgggtgg aacctgatgg cggtgcttgc gctggtgctg ggcgtggcgc
      181 cctgcctgcc ggggtatctg gtcgtatccg gggtgctgga tcagggtgcg gtggcgccgt
      241 tctgggtgac gctgttcgac ttcggctggt tcgtcagtct tgcgattgcc ggcgcggtga
      301 cgctgctggc gcgtccgcgc ggcgtgacac tggcgcgcgc cgtcggttag cggcgcccaa
      361 gagcaaggac gccgggcccc ggcttgcgcc ggggccacag ggaggagggg cgtcggcggt
      421 caggccgcca acagtgttcc cggcgagctg tgcttgcagc cgtcgagcag gaaggccagg
      481 ctgatcacca gcaccaactc accctcggcc gagcgtgcgg tgccgttgac gcccggaacg
      541 gcgattgccg tcagcggctt gatcacgaga tcggcggtgc cgtccacgcc ttccacggcg
      601 acgatgtacg gatgcggcgc ggcgatcacg atgccgacct tctcccggca cggctcgtag
      661 ccgaggatct cggcgagcga gcgcacggcg agcgggcggc cctggtcgcg cagcaccggg
      721 gcgccgccga cttcgtcaaa gctttccggc agttccacca cgcgctgcac gaccgccatc
      781 ggaatcgcca gcagcgcgcc ggaggtgcgc accagcatgg tcggcacgat cgacagttcg
      841 atcggcagac ggatcgtgaa cgtggttccc tcgcccaggt gcgagtcgat gcggatcgcg
      901 ccgcggtgct tctcgaccgc ggtgcgcacg acgtccatac cgacgccgcg gccggagacc
      961 gaggacgcca cttccttcgt cgagaagccg ggcaggaaga ccagctggaa cgcttcctcg
     1021 gcggtctgcg cctgatgttc gccgatcagg cccttggcca gcgccttcgc gcgcaggcgg
     1081 tccgcgtcca tgcccttgcc gtcgtcggac acttcgatca tcacgctgct gccttcctgc
     1141 caggccttga gcaggatggt cgccttggcc ggcttggcgg tggcggcgcg ttcctccgcc
     1201 gtttcgatgc cgtggtcgag cgcgttgcgc agcatgtgca ccagcgggtc gtagaggctg
     1261 tcgacgacca cgcggtcgac ttcggtctcc gcgccctcga tcaccaggtc cacgtccttg
     1321 ccgagatcgc gcgcgagttc gcgcaccagg cgcgggaatt tctggaacag gcggccgacc
     1381 ggctgcatgc gcgtcgaaag cgtcgcgcgc tgcagttcgg atgagtagcg cgatgcgcgg
     1441 ctcagggttt cctgcagcgt cgccatcagc gcggcggcct ggccctcgaa cttgaactgc
     1501 gcgagctttt ccagcagcac cgcggcctgg ttggctgcct gcaccgattc gccggccact
     1561 tccagcaggg cgtcgagctt gatcgcgtcg atgcggatcg tgtcttcctt gacctgcgtg
     1621 gtgatcgggc gcgcctcgtc ggcggccgcc ttgggctgcg ccgcgcccgt ggacggcacg
     1681 gcaggcgcgg cctgcgcggc agattccgct gccgggacgg cggcgctcgc cgggacgacg
     1741 gcgcgataca gcgcctccca gtcgggtccg tcggcgctgc cggcgcccgc atcgatggcg
     1801 ggaggcggct ggacagacgg cgccggctcg atagcgcgcg cgggagcgga cttgccctcg
     1861 atcgcctcgg tcagcagcac ttccagttcc ggcggcatcg acggcagcga atcgggcgaa
     1921 gcgccgttgg cgagctgctt cagctggtcg ccaacgaagc cgctggcctg caggccggct
     1981 tcgatcgccg acggcgtgac tggcaccttg ccggtgcgca gcgcatcgaa caggttctcg
     2041 gtcagatggc aggcggagac gatggcggtg aggttcatga agccggcgcc gcccttgatc
     2101 gtatggaagg agcggaacag cgcgttcagc gagtcgctgt cgtcgggccg ccgctccagc
     2161 gagagcaggt gttcctcgac gttggtcgcg aggtccagcg cctcgacgac aaactccttg
     2221 agcatgtcat ccatcagaac cccaggtcgg caagaagact gtccacgtcg tcctgcgcca
     2281 tggcctggcc cggcgcggac ggtccctgca tcagctcggc cggcttctgc tccatcgccg
     2341 cgcgtacctc gggcggggcg ttgtcgcgca gcagttgcgc gagttcgcgc tcggcggtgc
     2401 tggtgatact tacgatcttc ttgatcagct ggccggtaat gtcctggaag tcctgcgcca
     2461 tcatgatgtc gagcaggcgg gctttctcgg cttcggacgc attcatcaca tgggcggcga
     2521 actgccggga gtcgccggcg agggacttga attcctcgat gctgagcttg ccggcgaaca
     2581 gctgggtcca gcgttcgtcc atctcctgcg cctgcttcga cagcgcctcc tgctcgggca
     2641 ggctggtgtc gaccgtgttg agcaccttgt tggcggcctg ctcggtcagc gaggcaatgt
     2701 gctcgaggcg gctctgggca tcggtgatct ggctggccac gtccgacagc gagcggtcga
     2761 agccgagctc gcgcagcgaa tcgtgcagca tgcgcaggat gccgccgaga cgctcgtaca
     2821 tcgagttgtc gtgatcgtgg gcttcgggcg cggcgggcag ggcggtgctc gcaaggtcgg
     2881 ggttattggc tgcagcctct gccggcgggg cgggcggcgc ttccgcggcc gtggtcccgc
     2941 gcccggcgga aatctggtca aacagtgctt ccaggtcgtc gtctgcagcg tcgctcatcg
     3001 tggtgttccg tattcgttat caggtcatcc atgccagcct ccctgacatg acgactggcg
     3061 ctccccgccg ttgggacaga aaatttcgcc agtgcaacac gggccatgat actgcttcga
     3121 taatcgccag ccaagcagcg cgcgctttgg ctgtcccgct ctgttgctaa cgcgccagaa
     3181 gcgcaaggct tgccctgcac aatgcatggt cggggtcggc gaaggcgtcg cagatggtca
     3241 ggtggttgga ctgtggcagc ggcaccgttg tctgatgcgc atctttccat gcattttcga
     3301 tcagcgcggt ctggcgccgg aattcgtccg attcccgcgc gccgcaggcg gtcacgaaag
     3361 gaatcggtcg atgcggcggc atcagcgccg gcgacaggcg cgcgacgtcg gcctcgccga
     3421 tcttcaggtc gacattgacg aaatccgcat gccggaccgg ttcgaggtcg tagatgccgg
     3481 acatcaggac cgcgcccttg accaggtcgg cggggagatc gtccgcatac accggccagt
     3541 gcgcggccat catcatcgcc gccaggtgcg cgccggccga gtgcccggcc acgacgatgc
     3601 gcccggcgtc gaaatcgtat tgctcggcgt ggcgatacag ccaggcgagc gcacgcagct
     3661 gttctgcgac gatttcggcg accgatgcct gcggggcgag cgtgtagttg gtcagcgcga
     3721 cattgaatcc ggcacgcgtg taggccggca cgacgaagga gaaatccgac ttgtccagcg
     3781 agcgccacca tccgccgtgg atgaacacca gcagcggcgc attgccgcgc gacgcgggaa
     3841 agaagtcgag ccgttcgccg ctcgcgtcgc cgtaggcaat gtcgaacagt ccggccagct
     3901 ggcggcgcac ggcggccgaa tccttctgcc aacgcgtgaa gatgcggggg tgatccggga
     3961 tggtggcgcg ggcgttgtac tggcggttgt agaagtcggg atccggcttg gacatgaggg
     4021 tgattccgaa ggggcttgat cgggaggtgg agtgtactgc tttccggagg cggcgatcgg
     4081 gcgggtacaa tcccggctgt cacgggtgcc gcccgtgccc gcc
//
LOCUS       DATLGF010000321         3511 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_29929,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000321 DATLGF010000000
VERSION     DATLGF010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29929"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VM406_09820"
     CDS             <1..861
                     /locus_tag="VM406_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVL76303.1"
                     /translation="DLARLMLASSQVNAEGSPRMVPLRQVASIEQSTGASQINRRNLN
                     REVELSANVVGRSAGAVSADVRRALDAMDWQPGYRYQMGGATKNMQESFGFALSALFL
                     AIVFIYMILASQFASFTQPLAIMSALPMTLIGVFLALLMFRSTLNMFSIIGFIMLMGL
                     VTKNAILLVDFANKARAAGADRFSALMEAADVRLRPILMTTLAMVFGMVPLAFGMSEG
                     SEQRAPMGQAVIGGIITSSILTLVVVPVIYTYLDDFAAWAKRKWNGKPETVHGTPPLT
                     VPESKQEDAV"
     gene            902..1564
                     /locus_tag="VM406_09825"
     CDS             902..1564
                     /locus_tag="VM406_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HVL76304.1"
                     /translation="MNIEKARFNMIEQQIRTWDVLDPAVLETLVEVKREAFVPAAYKS
                     LAFVDTEIPLPGGENMFSPKMEARILQEVAPRKDEDVLEIGAGSGYMAALLASKARHV
                     TTVEIDPALRDLAAKNLSGYGVTNVDVVQGDGARGWNDPGRQTPWDVIVISGSLPLLP
                     EAFLKQIKVGGRVFSIIGTSPVMSAQITTRVGENAWDTVKIFETDVKPLRNAQAPSRF
                     SF"
     gene            1573..1896
                     /locus_tag="VM406_09830"
     CDS             1573..1896
                     /locus_tag="VM406_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HVL76305.1"
                     /translation="MQTVTAPELASWLADESRPKPVLLDVREPWEFATCHIAGSQPMP
                     MNTVPARIDELDEEAQIVCICHHGARSMQVAAFLERNGFADVINLTGGVHAWALQVDN
                     TMPTY"
     gene            1919..3220
                     /locus_tag="VM406_09835"
     CDS             1919..3220
                     /locus_tag="VM406_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HVL76306.1"
                     /translation="MRKPALAALASLIAGTLLSVHAHALDLMQVYQEALANDAQFASA
                     RATLTAGQERAVQARAGLLPVIGLGGAYSRADPPGPISAVNTNTYAISLSQPLFRLAN
                     WETYQQGKLSVAISEAQFAQAQQDLIVRVAQAYFDVLAAQDTLASVQAQKTAITEQLA
                     SAKRNFEVGTATITDTHEAQARYDLSIAQEFAAQNDLEIRRSALQQIIGRPAGELAQL
                     RPGVRLTPPEPAQVTNWVESAEQQNYGVLAQQLALEISRSEIKRNRAGHYPTLDLQAS
                     RSHSNRTSVPGAPTGGTNSVSLQWNIPLFTGFAVDSRVREAIALEDRARADLENVRRT
                     AAQGARQAFLGVNAGLAQVRALEAAEVSSQSALESNQLGYQVGVRINIDVLNAQQQLF
                     STRRDLARARYETIVNGLRLKSAAGTLGVDDVMQVNGLLAR"
BASE COUNT          640 a         1150 c         1141 g          580 t
ORIGIN      
        1 gatctggccc gcctgatgct cgccagctcg caggtgaacg ccgagggctc gccgcggatg
       61 gtgccgctac gccaggtcgc cagcatcgag caatcgaccg gggcgagcca gatcaaccga
      121 cgcaacctga accgggaggt agagctttcg gcaaacgtgg tcggccgttc ggccggcgcg
      181 gtcagcgccg acgtgcggcg cgcgctcgat gcgatggact ggcagccggg ctaccgctac
      241 cagatgggcg gcgcgaccaa gaacatgcag gaatcgttcg gtttcgcgct gtccgcgctg
      301 ttcctggcga tcgtgttcat ctacatgatc ctggcctcgc agttcgccag cttcacccag
      361 ccgctggcga tcatgtccgc actgccgatg acgctgatcg gcgtgttcct tgcgctcctg
      421 atgttccgct ccacgctcaa catgttctcg atcatcggct tcatcatgct gatgggcctg
      481 gtgaccaaga acgcgatcct gctggtcgat ttcgcgaaca aggcgcgcgc ggccggggcc
      541 gaccgcttct ctgcgctgat ggaggcggcc gatgtgcgcc tgcgccctat cctgatgacc
      601 acgctggcga tggtgttcgg catggtgccg ctcgccttcg gcatgtccga aggctccgag
      661 cagcgcgccc cgatgggcca ggcggtgatc ggcgggatca tcacctcgtc gatcctgacg
      721 ctcgtggtgg tgccggtcat ctacacctat ctggacgact ttgccgcatg ggcgaagcgc
      781 aagtggaatg gcaaaccgga aacggttcac ggaacaccgc cgctgaccgt gcccgagagc
      841 aaacaagagg atgccgtttg ataaaattcc cggtttccca acccaaccgc cgaatagcga
      901 catgaacatc gaaaaagccc gtttcaacat gatcgagcag cagatccgca cctgggacgt
      961 cctcgatccg gcagtgctgg aaacgctggt cgaggtcaag cgcgaagctt tcgtgccggc
     1021 cgcctacaag agcctggcct tcgtcgacac cgagatccct ttgccgggcg gcgaaaacat
     1081 gttctcgccg aagatggaag cgcgcatcct gcaggaagtc gcgccgcgca aggatgagga
     1141 cgtgctggaa atcggcgccg gctccggtta catggccgcc ctgctcgcca gcaaggcacg
     1201 ccatgtgacc accgtggaga tcgatccggc gctgcgcgac ttggcggcga agaacctgtc
     1261 cggctatggc gtgaccaacg tcgacgtggt gcaaggcgac ggcgcgcgcg gctggaacga
     1321 tcccggacgc cagacgccgt gggacgtgat cgtgatctcc ggttcgctgc cgctgctgcc
     1381 ggaagccttc ctgaagcaga tcaaggtcgg cggccgcgtg ttttccatca tcggcacttc
     1441 gccggtgatg tcggcccaga tcaccacgcg cgtcggcgag aacgcctggg acaccgtcaa
     1501 gattttcgaa accgatgtga agccgctgcg caacgcgcag gcgccttcgc gcttctcttt
     1561 ctgaggcata ccatgcaaac cgtgacagca ccggaactgg catcctggct ggccgatgaa
     1621 tcccggccca agccggtgct gctcgacgtg cgcgagcctt gggaattcgc gacctgccat
     1681 atcgccggat cgcagccgat gccgatgaac acggtgccgg cgcgtatcga cgagctggac
     1741 gaggaggcgc agatcgtctg catctgccac cacggcgcgc gcagcatgca ggtggctgcc
     1801 ttcctcgagc gcaacggctt cgccgacgtg atcaacctga ccggcggcgt ccacgcctgg
     1861 gcgctgcagg tcgacaacac gatgccgacc tactgagttc ccacacggag aaaacctgat
     1921 gcgcaaaccc gccctcgccg ccttggcttc gctgatcgcc ggcaccctgc tgtccgtcca
     1981 cgcccatgcg ctcgacctga tgcaggtgta ccaggaagcg ctcgccaacg atgcgcagtt
     2041 cgcgagcgcc cgcgcgacgc tgaccgccgg gcaggagcgt gcggtgcagg cgcgcgccgg
     2101 gctgctgccg gtgatcggcc tgggcggcgc gtacagccgg gccgatcctc ccggcccgat
     2161 atcggcggtc aataccaata cctatgcgat ctcgctctcg cagccgctgt tccggctggc
     2221 gaactgggag acctatcagc agggcaagct gtcagtggcg atcagcgagg cgcagttcgc
     2281 gcaggcgcaa caggatctga tcgtgcgcgt cgcgcaggcc tacttcgacg tgctggccgc
     2341 gcaggacacg ctggcttcgg tgcaggcgca gaagaccgcg atcaccgaac agctggcttc
     2401 ggccaaacgc aacttcgagg tgggcaccgc gacgattacc gatacccatg aagcgcaagc
     2461 ccgctacgac ctttcgatcg cccaggaatt cgcggcgcaa aacgatctcg aaatccgccg
     2521 ttcggccctt cagcagatca ttggccgtcc ggccggggag ctcgcgcagc tgcgtccggg
     2581 cgtgcgcctg accccgccgg aaccggcgca ggtgacgaac tgggtggaga gcgccgagca
     2641 gcagaactac ggcgtgctcg cccagcagct tgcgctggag atctcgcgca gcgaaatcaa
     2701 gcgcaaccgt gccggccact atccgacgct ggatctgcag gccagccgca gccacagcaa
     2761 ccgtaccagc gtccccggtg cgcctaccgg cggcaccaat tcagtttcgc tgcagtggaa
     2821 catcccgctg ttcaccggat ttgcggttga cagccgcgtg cgcgaggcaa tcgcgctgga
     2881 agaccgcgcc cgcgccgatc ttgagaacgt acgccgcacc gccgctcagg gcgcgcgcca
     2941 ggccttcctc ggcgtcaatg ccggcctcgc acaggtgcgc gcactggaag cggccgaggt
     3001 gtcgagccag tcggcgctgg aatcgaacca gctcggctac caggtcggcg tgcgcatcaa
     3061 catcgacgtg ctgaatgcgc agcagcagct gttctccacc cggcgcgatc tggcgcgggc
     3121 acggtatgag acgatcgtca acggtctgcg gctgaagtcg gccgcgggga cgctaggcgt
     3181 ggatgatgtg atgcaggtga atgggctgct ggcgcgttga gcgcagactt ggcaagcgag
     3241 gacgctgagc aagcgaggac gcaaagtgag cggatgaagt ccctcgcatc gctcgggacg
     3301 acagtgttgg gggttatcgc tatattcctg tcgtcccgaa cgcagtgagg gcggcccgcg
     3361 tcagggacct catcgccctg gcaagcgagg acgcaaagcg agcggatgag attcctcgtt
     3421 tcgctcggaa cgacagcgtt ggggatcatc cccaaactac tgtcgtcccg aacgcagtga
     3481 gggcggcccg cgtcagggac ctcatcggcc c
//
LOCUS       DATLGF010000322         5247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_212914, whole genome shotgun sequence.
ACCESSION   DATLGF010000322 DATLGF010000000
VERSION     DATLGF010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212914"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /gene="cobT"
                     /locus_tag="VM406_09840"
     CDS             <1..1035
                     /gene="cobT"
                     /locus_tag="VM406_09840"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HVL76307.1"
                     /translation="MPVPRIAHTADAEIEAALRRAIDSKTKPLGSLGVLEALALQIGL
                     IQRSVAPCVKTPAVLVFAGDHGVVEEGVSAYPQSVTWQMVENFLAEGAAINVFARRNG
                     CRLLVVDAGVNHDFGPRTGLLDRKLGPGTRNFAREPAMTRAQCELAMRYGAGIVAELD
                     GNVLGFGEMGIGNTSAASALMHRLTGASLDDCVGAGAGLDAPGIAHKRAVLERAIARH
                     AGVHEPLDLLATFGGFEIAMMAGAMLAAAERRMVLLIDGFIVTSALLVASRLQPAILD
                     YCVFSHCSDERGHRQMLENLGARPLLQLGLRLGEGSGAALALPLVQAAVDFLGEMATF
                     ESAQVSGKEA"
     gene            1038..1844
                     /locus_tag="VM406_09845"
     CDS             1038..1844
                     /locus_tag="VM406_09845"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HVL76308.1"
                     /translation="MSVPATPSGILRRALFQLRLFFIALQFFTRLPIPAWVGFESHWL
                     HLASRYFPAVGIVVGAAAALVFLAADAVLPTLPAVLLSTAAGIYLTGAFHEDGFADTC
                     DGLGGGTTRDRVLEIMTDSRIGTYGALGIGLLLAAKVAALAHLPAQLVAPVLVAAHPL
                     SRLLSTALIWKLDYVKGSGKAKPLAQRISNGEFAIALGSVALTVAALVAAGLLAWQAL
                     VAGLIAAALAAAWLARKCVLRIGGYTGDCLGAVQQLSEAAFYIGVLALVT"
     gene            1846..2403
                     /locus_tag="VM406_09850"
     CDS             1846..2403
                     /locus_tag="VM406_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HVL76309.1"
                     /translation="MRLYLIRHPRPVVAAGTCYGSTDLCVSEREVAQVTGALLPLLPR
                     GASFYSSPLLRCMALARPLAQALGGMPIVEDARLVELNFGSWENRAWDAISRAEVEAW
                     AGDPVRYRPGGGESVLDAALRVQAFLDHVRSAGEGSAIVVCHAGVIRLAHASRHGADA
                     EAAARMAADRRQPSPAYGSLTLIDL"
     regulatory      2424..2645
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2741..4723
                     /locus_tag="VM406_09855"
     CDS             2741..4723
                     /locus_tag="VM406_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVL76310.1"
                     /translation="MPFRFLIMNTSILQRTSVSRPLVLVAAISSLFLLPADAAIAQAQ
                     QARVLSPVTVSGTRFETEAARAPIGASVISGDEIREAGINNVNEAIRRLGGVHGRLNF
                     AGTQDYSLDLRGFGTNSDQNLVILVDGIRLNENELSPALLSSIPIESVERIEIVRGGS
                     SVLYGEGATGGTIQIITRRGAARGLNGTVVGEAGSLNHRELRASVAYGWDGFSIDAHA
                     GTQRADNFRENNDVRLDNFSGGLQWASAGTRLGLRIDASRQDSRFAGPLSLAQFRQNP
                     RQTLTPDDFGSFDSDRYTFFAEQRVGAFDFAAELSRRERRTESSFGGFAWESDSKTTQ
                     FSPRLRHRADFGGVGNELVAGVDVARWERSAGGTDDLQRSRAVYVRDEVRIGEARIAA
                     GARREVFDQESAFGAFQSYDVSRSLNAWELQGSYAFAPTLNVFAKTGRSFRVANADEH
                     GFTATGQPLAPQTSRDLELGVTLGQAGQRLTARVFQHRLRNEIFFDPTAPNAFGGTGA
                     NANLDPTRRRGVELEGQLALARDFLLSASFQHVSAEFTGGANAGNEMVLVPRNTALAR
                     LHWTPGSAHSAYVGLQWTDSQRYGGDFANACTTRIPSYTLLDARYAYRTGPWEFAVVG
                     TNLTDRRYFSNAFGACESGIYPDPGRQVRVTVRYDF"
     regulatory      4749..4971
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          803 a         1740 c         1769 g          935 t
ORIGIN      
        1 atgcccgttc ccaggattgc ccataccgcc gatgcagaaa tcgaagccgc gctgcggcgc
       61 gcgatcgaca gcaagaccaa gccgctcggc agccttggtg tgctcgaggc gctcgcgctg
      121 cagatcgggc taatccagcg cagcgtcgct ccctgcgtga agacgcccgc tgtgctggtg
      181 tttgcgggcg accatggcgt ggtggaagag ggggtctcgg cctatccgca gagcgtgacg
      241 tggcagatgg tggagaactt cctggccgaa ggggccgcga tcaatgtctt tgcccgccgc
      301 aacggatgcc ggcttctggt ggtcgacgcc ggcgtcaacc atgacttcgg accgcgcacc
      361 ggcctgctcg accgcaagct cgggcctggc acgcgcaatt tcgcgcgcga gccggcgatg
      421 acgcgcgcgc aatgcgagct ggcgatgcgc tacggcgcgg ggatcgttgc ggaactggac
      481 ggcaatgtgc ttggtttcgg cgagatgggc atcggcaaca cgagcgcggc gtcggcgctg
      541 atgcacaggc tcaccggcgc ttcgctggac gactgcgtcg gcgccggggc cggattggat
      601 gcgcccggca ttgcacacaa gcgcgcggta ctcgagcggg caattgcgcg tcacgccggc
      661 gtgcatgagc cgctcgatct gctcgccaca ttcggcggtt tcgagatcgc gatgatggcc
      721 ggcgcaatgc tcgctgccgc cgagcgtcgc atggtgctgc tgatcgatgg cttcatcgtc
      781 accagtgcgc tgctggtcgc ctccaggctg cagccggcca tactcgacta ctgcgtgttc
      841 tcacattgtt cggacgagcg cggtcatcgc cagatgctgg aaaacctggg cgcccgaccg
      901 ctgctccagc tcggcctgcg cctgggggag ggcagcgggg cggccctggc gctgccgctg
      961 gtgcaggcgg ccgtcgactt cctgggcgag atggcgacct tcgaatccgc gcaggtcagc
     1021 gggaaagagg cttgaaggtg tccgtgccgg ccacgccctc gggaatcctg cggcgcgcgc
     1081 tgttccagtt gcggctgttc ttcattgcgc tgcagttctt cacgcggctg ccgatcccgg
     1141 catgggtggg cttcgaatcg cactggctgc atctggcgag ccgctatttt ccggcggtcg
     1201 gcatcgtggt cggcgctgcg gccgccctcg tcttcctcgc cgcagatgcg gtcctgccca
     1261 cgctgccggc ggtgctgctg tcgaccgctg ccggcatcta cctgaccggc gcgtttcacg
     1321 aggacggttt cgccgatacc tgcgatggcc tcggcggcgg taccacccgc gaccgcgtgc
     1381 tggaaatcat gaccgattcc cgcatcggga cctacggagc gctcgggatc ggactgttgc
     1441 tcgcggcgaa ggtcgccgcg ctggcgcatc tcccggcgca gctggttgcg cccgtgttgg
     1501 tagccgcgca tccgctctcc cgcctgctgt cgaccgcgct gatctggaag ctcgactatg
     1561 tgaaggggag cggcaaggca aaaccgctcg cgcaacgcat ctcgaacggc gaattcgcga
     1621 ttgcgctggg gagcgtcgcg ctgaccgtgg cggcgctggt cgcggccggt ctgcttgcgt
     1681 ggcaggctct cgtcgccggg ctgatcgccg ctgcgctcgc ggccgcctgg ctggcccgca
     1741 agtgcgtgct gcgcatcggc ggatataccg gcgattgcct cggcgccgtc cagcagctgt
     1801 ccgaagcggc gttctatatc ggcgtgctag cgctggtgac ctgacgtgcg tctctacctg
     1861 atccgccatc ctagaccggt cgtcgccgcc ggcacctgct acggcagcac cgacctgtgc
     1921 gtctccgaac gcgaggtggc gcaggtgacg ggagcgctgc tgccgctgct gccgcgcggc
     1981 gcgtcgttct actccagtcc cttgctgcgc tgcatggcgc tcgcgcggcc gcttgcgcag
     2041 gcgctcggcg gcatgcccat cgtcgaggac gcgcgcctgg tcgagctgaa cttcggctcc
     2101 tgggagaacc gtgcatggga cgcgatttcc cgtgcggagg tcgaagcctg ggcaggggac
     2161 ccggtgcgct accggcccgg aggcggggag agcgtgctgg atgcggcgct gcgcgtacag
     2221 gcgtttctgg accatgtgcg cagcgccggc gaaggcagcg cgatcgtggt ctgccatgcg
     2281 ggcgtcatcc gtcttgcgca cgccagccgg cacggggccg atgccgaggc tgcggcacga
     2341 atggctgctg accgacggca gccatcgccc gcatacggat ccctgacgct gatcgatttg
     2401 taagcaattc atgcaagaaa gccttacaat cgcaccgttc tggtgcccgc ccgcgcgttc
     2461 gcgcgggcag ttaaacggga aacacggcgc cccttcacgg gccaacgtgt gctgcccccg
     2521 caacggtaag caagcgtcag cgatgcgctg acaaccggtc cttcacgcca ctgtgcgaag
     2581 agcatgggaa ggtaggccgg ttcgacttgc aagcccggat accggccagg acaggtggaa
     2641 gtcgacgagc gggtggctcg gcatcgaatc cggtgagccc gatccgaatc cgcatccgtt
     2701 ccgacttctg atgaattccg cctgcgggga agcaggcggg atgccgtttc gcttcctgat
     2761 catgaacact tccatcctcc agcgcacgtc ggtgtccagg ccgctcgtgc ttgtcgccgc
     2821 catctcctca ttgttcctgc tgccagcgga cgcagccatc gcgcaagccc agcaagcgcg
     2881 cgtgctgtcg cccgtgactg tcagcggcac gcgctttgaa accgaagcgg cgcgtgcgcc
     2941 gatcggcgcg agcgtaatca gcggtgacga aatccgcgaa gccggcatca acaacgtcaa
     3001 tgaggcgatt cgccgcctcg gcggcgtgca tggccggctc aacttcgccg gcacccagga
     3061 ttactcgctc gacctgcgcg gcttcggtac caacagcgac cagaacctgg tgatcctggt
     3121 ggacggcatc cgcttgaatg agaacgagct gagcccggcg ctgctgagtt cgatcccgat
     3181 cgaatcggtc gagcgtatcg agatcgtgcg cggcggcagc agcgtgctgt acggcgaggg
     3241 cgccaccggc ggcacgatcc agatcatcac ccgtcgcggc gcggccaggg ggttgaacgg
     3301 aacggtggtc ggcgaggcgg gttcgttaaa tcaccgggaa ttgcgtgctt cggttgccta
     3361 cggatgggac ggtttttcca tcgatgcgca tgcagggacg cagcgcgcgg acaacttccg
     3421 cgagaacaac gacgtgcggc tggacaactt cagcggcggc ctgcagtggg catccgccgg
     3481 cacccggctg ggccttcgca tcgatgcctc gcggcaggac tcgcgctttg ccggcccgtt
     3541 gtcgcttgca cagttccggc agaatccgcg ccagacgctg acgccggacg acttcggttc
     3601 tttcgatagc gatcgctaca cgttctttgc cgagcagcgc gtcggcgcgt tcgacttcgc
     3661 cgccgagctg tcgcggcgcg agcggcgcac cgaatcctcg ttcggcggct tcgcctggga
     3721 gtcggacagc aagaccactc agttctcgcc gcggctgcgc catcgcgccg acttcggcgg
     3781 cgtaggcaac gaactggtgg ccggggtgga tgtcgcccgc tgggagcgca gtgcgggtgg
     3841 cacggacgac ctgcagcgca gccgcgcggt gtacgtgcgc gacgaggtcc gcatcgggga
     3901 ggcgcgcatc gcggccggcg cgcgccgcga ggtgtttgac caggaatccg cattcggcgc
     3961 attccagtca tatgacgtct cgcgctcgct caatgcgtgg gaattgcagg gcagctatgc
     4021 atttgcgccg acgctgaacg tcttcgcgaa aaccggccgc agcttccgtg tggcaaatgc
     4081 cgacgaacat ggatttaccg ccacaggcca gccgctcgcc ccccagactt ccagggatct
     4141 cgaactcggc gtgacgctgg gccaggccgg gcagcgcctg accgcgcgcg tattccagca
     4201 ccggctgcgc aacgagattt ttttcgatcc caccgcgccg aatgctttcg gcggaacggg
     4261 tgcgaatgcc aatctggacc cgacccgccg ccgcggcgtc gagctcgagg gccagctggc
     4321 gctcgcgcgc gacttcctgc tcagcgcgag cttccagcat gtgtcggccg agttcaccgg
     4381 cggtgcgaac gccggcaacg aaatggtgct ggtgccgcgc aacaccgcgc tggcccgcct
     4441 gcactggacg ccgggcagcg cgcacagcgc gtatgtcgga ctgcagtgga ccgattcgca
     4501 gcgctacggc ggcgacttcg ccaatgcctg cacgacgcgc attccgtcct atacgctgct
     4561 ggatgcgcgt tatgcgtatc gcaccggtcc gtgggaattc gccgtggtcg gcaccaacct
     4621 gactgatcgc cgttacttca gcaacgcgtt cggcgcctgc gaatcaggca tctacccgga
     4681 cccgggtcgc caggtccggg tgacggttcg ctacgatttc tgatgttgcc tgtcggttat
     4741 ctgtagcctt acaatcgcaa cgctctggtg cccgcccgca caatcgtgcg ggcagttaaa
     4801 cgggaaacac ggcgctccaa gaacgagcca acgtgtgctg cccccgcaac ggtaagcagg
     4861 cgtcgtccgc gtgacgacag gtcgcctcca catgccactg tgcgctttgc atgggaaggt
     4921 gagacgacca ggcctgcagc ccggataccg gccagagcag gtggaagtgt gcgaacgggg
     4981 atgtttcgtg acgcggatcg ggctgctgca tgcggctcat tctcttcatc cgacgatttc
     5041 cgttccagct tctgttcgat ccggcccgcg gggaagcggg ccggtcgctt tcaatcggaa
     5101 atcatccatg tcccgttcca caggtccggc cgccttcccg gcgcctttcc tcccgctcgt
     5161 tgcgagcatc ctgtccgccg ttcccgccca tgtcgccgcc acggaaaaca ccttggcgcc
     5221 ggttttcgtc accgcctcgc gcacgcc
//
LOCUS       DATLGF010000323         3836 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_181888, whole genome shotgun sequence.
ACCESSION   DATLGF010000323 DATLGF010000000
VERSION     DATLGF010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181888"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VM406_09860"
     CDS             <1..723
                     /locus_tag="VM406_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HVL76311.1"
                     /translation="IHIQLQGTAGQSAGAFLAHGITLDLVGEGNDYVGKGLSGGRIIV
                     RPNTEFRGWAVDNIIVGNTVLYGAISGEAFINGVAGERFAVRNSGATTVIEGTGDHGC
                     EYMTGGTVVVLGATGRNFAAGMSGGIAYVYDPDGSFQSRCNMAMVALDPVLSAVDQEA
                     KIERALWHSTTRGGEGQTDEAILKGLIERHFKYTGSTRARNLLDDWTNARSKFVKVFP
                     TEYKRALGELNAAQSAKEKIAA"
     gene            749..2212
                     /locus_tag="VM406_09865"
     CDS             749..2212
                     /locus_tag="VM406_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HVL76312.1"
                     /translation="MGKVTGFLEYQRLQEASEAPEKRKKHYKEFVLHLSDDQAKIQGA
                     RCMDCGIPFCNNGCPVNNIIPDWNDLVYHGHYREALDNLHSTNNFPEFTGRICPAPCE
                     AACTLGINNDPVGIKSIEHFIIDKGWENGWVVPQPPKAKTGKKVAVVGSGPAGLAAAQ
                     QLARAGHDVTVFEKSDRVGGLLRYGIPDFKMEKSLIDRRVEQMQAEGVSFRTGVFVGK
                     DFTANVYNWAKETISPDQLKKDFDAVIIAGGAEQPRDLPVPGRELKGVHFAMEFLPLQ
                     NKVNAGDKVKDQIMATGKHVVVIGGGDTGSDCVGTSNRHGAASVTQFELLPQPPEQEN
                     KPLVWPYWPTKLRTSSSHEEGCERDWAVATKRLEGKNGKVEKLIAARVEWKDGKMVEV
                     PGSEFELKADLVLLAMGFVSPVQAVLDAFGVDKDGRGNAKATTDGDGCYKTSVDKVFA
                     AGDMRRGQSLVVWAIREGRQCARAVDEYLMGSTVLPR"
     gene            2348..3151
                     /locus_tag="VM406_09870"
     CDS             2348..3151
                     /locus_tag="VM406_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL76313.1"
                     /translation="MPHLVEIRGLHFSYGPRPILAGLDMDFPRGKVVAVMGGSGSGKT
                     TVLRLIGGQLRAQQGAIRFDGKEVDPTDSDNLYAMRRKMGMLFQHGALFTDLSVFENV
                     AFPLREHTRLPEVLIRDLVLMKLHAVGLRNAAHLKPAEISGGMARRVALARAIALDPQ
                     LIMYDEPFAGLDPISMGVAANLIRRLNDALGSTSIVVSHDVHETFHIADYVYFLSQGR
                     IVAGGTPQQMLASNDPYVRQFVHAEADGPVPFHYPGRSLAEDLGLERAQ"
     gene            3148..>3836
                     /gene="mlaE"
                     /locus_tag="VM406_09875"
     CDS             3148..>3836
                     /gene="mlaE"
                     /locus_tag="VM406_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HVL76314.1"
                     /translation="MIASTLAALGRQVRETVAGLGYAARMFVSLVAAFAGLLRRPRLV
                     TDQVHFIGNYSLVIIAVSGLFVGFVLGLQGYYTLTRYGSEQALGLLVALSLVRELGPV
                     VTALLFAGRAGTSLTAEIGLMKAGEQLSAMEMMAVNPMKRVVAPRFWAGVIAMPVLAA
                     IFSAVGVLGGYVVGVRMLGVDEGAFWSQMQAGVDVWADIGNGVIKSVVFGFAVTFIAL
                     YQGYEAQATPEG"
BASE COUNT          683 a         1172 c         1283 g          698 t
ORIGIN      
        1 atccacatcc agctgcaggg cacggccggt cagtcggcgg gcgccttcct cgcgcacggg
       61 atcacgctgg atctggtcgg cgaaggcaac gactacgtcg gcaaggggct gtcgggcggg
      121 cgcatcatcg tgcgcccgaa caccgagttc cgcggctggg cggtggacaa catcatcgtc
      181 ggcaataccg tgctctatgg cgcgatctcc ggcgaggcct tcatcaacgg cgtggcgggc
      241 gagcgctttg ctgtgcgtaa ttccggtgcg acgacggtca tcgaaggaac cggcgaccat
      301 ggctgcgaat acatgaccgg cggcacggtc gtcgtactgg gcgcaaccgg tcgaaacttc
      361 gcggccggca tgtcgggcgg gatcgcctat gtgtacgacc cggacggcag cttccagtcc
      421 cgctgcaaca tggcgatggt ggcgctggac ccggtcctct cggccgtcga ccaggaagcg
      481 aagatcgagc gcgcgctgtg gcacagcacc acgcgcggtg gcgaaggcca gaccgacgag
      541 gccatcctca aaggcctcat cgaacgccac ttcaagtaca ccggcagcac gcgcgcgcgc
      601 aacctgctgg acgactggac caatgcacgc agcaagttcg tcaaggtgtt cccgaccgag
      661 tacaagcggg cactgggcga gctgaatgcg gcccagtcgg ccaaggaaaa gatcgcggcg
      721 taatcaggat taggaaagaa agagaatcat gggaaaagtc accggcttcc tggaatacca
      781 gcgcctgcaa gaggcaagcg aagcgcccga gaagcgcaag aagcactaca aggaattcgt
      841 cctccatctg tccgacgacc aggcgaagat ccagggcgcg cgctgcatgg attgcgggat
      901 ccctttctgc aacaacggct gcccggtcaa caacatcatt ccggactgga acgacctggt
      961 ctaccacggc cactatcggg aggccctgga caacctgcac tccaccaaca acttccccga
     1021 gttcaccggg cgcatctgtc cggctccgtg cgaagccgcc tgcacgctgg gcatcaacaa
     1081 cgacccggtg ggcatcaagt cgatcgagca tttcattatt gacaagggct gggagaacgg
     1141 ctgggtcgtc ccgcaaccgc caaaggcgaa gaccggcaag aaggttgccg tggtgggctc
     1201 cggtccggcc ggtctggctg cggcccagca gctcgcgcgt gccggtcatg atgtgacggt
     1261 gttcgaaaag agcgatcgcg tcggtggcct gctgcgctac ggcattcccg acttcaagat
     1321 ggagaagtca ctgatcgatc gccgcgtcga gcagatgcag gctgaaggcg taagcttccg
     1381 caccggcgtc tttgtcggca aggatttcac ggccaacgtc tacaactggg ccaaggaaac
     1441 gatttcgccg gaccagctga agaaggactt cgacgcggtg atcatcgccg gcggcgccga
     1501 gcagccgcgc gacctgccgg tacccggccg cgagctcaag ggcgtgcatt tcgcgatgga
     1561 gttcctgccc ctgcagaaca aggtcaacgc cggagacaag gtcaaggacc agatcatggc
     1621 caccggcaag cacgtggtcg tgattggcgg cggcgatacc ggttccgact gtgtcggcac
     1681 ctcgaaccgc cacggcgccg cctcggtcac ccagttcgag ctgctaccgc agccgccgga
     1741 gcaggagaac aagccgctgg tgtggccgta ctggccgacc aagctgcgca cctcatcctc
     1801 ccatgaggag gggtgcgagc gcgactgggc ggtggcgacc aagcgtctgg aaggcaagaa
     1861 cggcaaggtc gagaagctga tcgccgcgcg cgtcgaatgg aaggacggca agatggtcga
     1921 ggtgcccggc tcagaattcg aattgaaggc cgacctggtg ctgctggcca tgggcttcgt
     1981 gtctccggtg caggctgtgc ttgatgcgtt cggggtcgac aaggacgggc gcggcaatgc
     2041 caaggcaacg accgatggcg acggctgcta caagacttcg gtcgacaagg tgttcgcggc
     2101 cggcgacatg cgccgcggtc agtcgctcgt ggtctgggcg atccgggaag ggcggcaatg
     2161 cgcccgcgcg gtcgatgaat acctgatggg cagcaccgtc ctgccgcgtt aatctggccg
     2221 aacttccagc aaaacccgcc gctctgctcc ggcgggtttt gccgttttag gtacaccgct
     2281 cggttcaccc ggccggttga atcctgtttc tgcactaaaa ttccgttttt tcctccaggc
     2341 ccttgccgtg ccccatctcg tcgaaatccg cggtttgcat ttttcctatg gcccgcgccc
     2401 gatcctggcc ggcctggaca tggatttccc ccgcgggaag gtggttgccg tcatgggcgg
     2461 ctccggctcg ggaaagacca cggtactgcg cctgatcggc ggtcagttgc gcgcgcagca
     2521 gggtgctatc cgattcgacg gcaaggaagt cgatccgacg gactcggaca atctgtatgc
     2581 gatgcggcgc aagatgggca tgctgtttca gcacggcgcg ctgtttaccg acctgtcggt
     2641 attcgagaat gtggccttcc cgctgcgcga acacacgcgt ctgccggaag tgctgatccg
     2701 cgatctggtg ctgatgaagc tgcatgcggt cggtctgcgc aatgccgccc atttgaaacc
     2761 ggccgagatc tcgggcggca tggcccgccg cgtggcgctg gcgcgggcaa tcgccctcga
     2821 tccgcaattg atcatgtatg acgagccgtt cgccggactc gatccgattt cgatgggtgt
     2881 ggccgccaac ttgatccgac gcctgaacga tgcgctgggg tcgacttcga tcgtcgtctc
     2941 gcacgacgtg cacgaaacct tccacatcgc tgactacgtc tacttcctgt cgcaggggcg
     3001 catcgtcgcc ggcggcacgc cgcagcagat gctggcgtcg aacgaccctt acgtgcgcca
     3061 gttcgtccat gccgaggcgg acggtccggt gccatttcat tatcccggcc gctcgctggc
     3121 cgaggatctc gggctggagc gtgcgcaatg atcgcctcga ctctggcagc actcggccga
     3181 caggttcgcg agaccgttgc gggtttgggc tacgcggcgc gcatgttcgt gtcactggtg
     3241 gccgcgtttg ccggcctgct ccggcgtcct cggctggtga ccgaccaggt gcatttcatc
     3301 ggtaattatt cgctggtgat cattgcggtg tcggggctgt tcgtcggttt cgtgctcggc
     3361 ctgcaaggtt attacacgtt gacgcgttac ggctccgaac aggcgctcgg tctgctggtc
     3421 gcgctgtcgc tggtacgcga actcgggccg gtggtcacgg cgctgctgtt tgccggccgc
     3481 gccggcacgt cgctcaccgc cgaaatcggg ctgatgaagg ccggcgaaca gttgtcggcg
     3541 atggagatga tggcggtcaa tccgatgaag cgcgtggtcg cgccgcgttt ctgggcgggc
     3601 gtgattgcaa tgccggtgct tgccgccatc ttcagcgcgg tcggcgtgct gggcggctac
     3661 gtggtcggcg tgcggatgct gggcgtggat gaaggcgcgt tctggtcgca gatgcaggcc
     3721 ggcgtcgacg tctgggccga tatcggcaac ggcgtgatca agagcgtggt gttcggcttc
     3781 gccgtgacct tcattgccct gtatcagggc tacgaggcgc aggcgacgcc ggaagg
//
LOCUS       DATLGF010000324         4902 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_139479, whole genome shotgun sequence.
ACCESSION   DATLGF010000324 DATLGF010000000
VERSION     DATLGF010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139479"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VM406_09880"
     CDS             <1..727
                     /locus_tag="VM406_09880"
                     /inference="COORDINATES: protein motif:HMM:NF017116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HVL76315.1"
                     /translation="FRMGEKDGYLAIQGILIYEIAELEQFNRAEVTAVKAFMSSQKDR
                     YREPYGRRMVNQPRRTVFAATTNEGQYFKDTTGNRRFWPVETGRIDLEGLRRDREQLL
                     GEAVHMMRAGVKWFPTRDEQQRLISPVQGDREIWDEWTGRVWEYLEGKDIEGNDTPLG
                     RRNDVTARELLTRALHIEIGKISNAKTETMRISNIMRKLGWQKRRQTGGAREWYYVRP
                     QEEKAPAQPEGLPAWEEGHDLPI"
     gene            1082..1444
                     /locus_tag="VM406_09885"
     CDS             1082..1444
                     /locus_tag="VM406_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76316.1"
                     /translation="MNSLREQMPETAAFLDALRDAFGKDAIDAQVRAAFAGAPTFWAM
                     EGRRLGVRITEHTCEVYRDARDMARARRARWMDDAQEVAQRMGIEIPRADVDDHQALQ
                     AEAQALREMIASMRRKAA"
     gene            1633..2190
                     /locus_tag="VM406_09890"
     CDS             1633..2190
                     /locus_tag="VM406_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76317.1"
                     /translation="MNNLSPQLEQWLDQLRGTPSSTVNFEGLSANDVRAQCAMILAAV
                     RTKLPEPEMWAIHAKYAATEHEETGPRLASGEVRARRYAFSAMKIHAIEQLVGWLVQT
                     SALREVPLAAMKCMVAKFYSNHAKVEISFRALAQEFGGNHMLYARTYKQLVVLLRPLE
                     ILAVRRLEPYFLQQGIVGDQQACTV"
     gene            2634..2954
                     /locus_tag="VM406_09895"
     CDS             2634..2954
                     /locus_tag="VM406_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HVL76318.1"
                     /translation="MQQQQDERRGSAHDRGYDSKWRKARDAYLREHPVCECDDCKRLD
                     WPPASEVVDHIIPHRLKDAKDSGNPDAIARAQELFWNRRNWKAMSKRCHDRKTAREDG
                     GFGR"
     gene            3065..3643
                     /locus_tag="VM406_09900"
     CDS             3065..3643
                     /locus_tag="VM406_09900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HVL76319.1"
                     /translation="MGLRGPKAKPTELKVLEGNRGHRPLITDGQLRPLVEIPDPPKHL
                     GKEARKEWKRITVELVRYNIVSRLDQQMLGMLCQAVERVALFETALQRRMLQIIQQNK
                     EQPAGKEQDPAQAYVSRTPQGYEMQCIEYQLLNKEREVLMKLLGEFGLSPAMRARVVL
                     GQPGAKQLTLFEGGKSDPEPKKNDGPQSFAEF"
     gene            3643..>4902
                     /locus_tag="VM406_09905"
     CDS             3643..>4902
                     /locus_tag="VM406_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HVL76320.1"
                     /translation="MDYVDRARRYAERVVAGEIIACKWVRLACKRQLDDLKREGWKYR
                     FDPSKANRVCRYLELLPHIKGDWARPVYVDGVAVFPTIRLEDWQCFIVCTVFGWVDER
                     GKRRFKRVYIEVPRKNAKSTISAGVALFMLTADGEPGAEIYSAATTGEQARIVFDDAK
                     RMALRSPALLERFGVSVMTYDITIAEMASTFRALNAEGSTLDGLNIHCAIVDELHAHK
                     RRELYDVLDSGTGSRSQPLLWMITTAGSDRSGICYEQRTHVTKVLDQVFEDESFFGII
                     FTIDEGDDWADPSVWSKANPNFGVSVLPDDMEAACRKAMSMPSAVNGFLTKRLNVWVN
                     ADSAWMDMRAWDACGDPSLKLEQFEGEECLIAHDLASKVDIADKVRLFWRDIDGERHY
                     YAFAQHYLNEQAVEEGRNSQYSGWARRG"
BASE COUNT          968 a         1410 c         1673 g          851 t
ORIGIN      
        1 tttccgcatg ggtgagaagg acggctatct ggcgatccag ggaatcctga tctacgagat
       61 cgcggaactg gagcagttca atcgggctga ggtcaccgcc gtcaaggcgt tcatgtcgag
      121 ccagaaagac cggtaccgcg agccgtacgg gcgccgtatg gtcaatcagc cgcgccgcac
      181 cgtcttcgcg gctactacca acgaagggca gtacttcaag gacacgactg gcaaccggcg
      241 cttctggccg gtggagaccg ggcgcatcga tctggagggt ttgcgacgcg atcgcgagca
      301 gctgctgggc gaggcggtgc acatgatgcg cgccggggtg aagtggttcc cgacgcgcga
      361 tgagcagcag cggctgatca gcccggtgca gggtgatcgc gagatctggg acgagtggac
      421 cggccgcgtg tgggagtacc tcgaaggcaa ggacatcgag ggcaacgata cgcccttggg
      481 gcgccgcaat gacgttaccg cgcgtgagct gctgacccga gccttgcaca tcgagatcgg
      541 caagatcagc aatgccaaga cggagaccat gcggatctcc aacatcatgc gcaagctcgg
      601 ctggcagaag cgccggcaga ccggcggtgc tcgcgagtgg tactacgtgc ggccgcagga
      661 ggaaaaagcg ccggctcagc ctgagggcct gccggcatgg gaggagggcc atgaccttcc
      721 gatctgaggt ggaggccacg gtgccgccag ttcgccaggg atcgtgcgcg gtacagccgc
      781 gtgcggaaaa ggcgatggcg ctccgttcag gcgtccaacc tcctcacctc gtccgacctc
      841 gaaaacgaag gttggacggc cgaaacccgc ataaacaggg gcttcgtcca acctcccaac
      901 ctttttggcg aagtctcacg cacacgcatg cacgcacacg tatacgcgtg acggttcccc
      961 gtgtcttttt tttcggtgaa acatcaaaac aggttagacg ggttaggagg ttagacgaag
     1021 ccagcattca tgcgggtttt cgccgtccaa ccttccgtcc aacttcaagg aggttagacg
     1081 gatgaacagt ctcagggagc agatgccaga aaccgcagcg ttcctcgatg ccctgcggga
     1141 tgcattcgga aaggacgcga tcgatgccca ggtgcgcgca gccttcgccg gtgcgccgac
     1201 gttctgggcg atggaggggc gcaggctggg ggtgcggatc accgaacaca catgcgaggt
     1261 gtatcgcgat gcgcgcgaca tggctcgggc aagacgggct cgctggatgg acgacgcgca
     1321 ggaagtggcg cagcgcatgg gaatcgaaat cccacgcgct gacgttgacg atcaccaggc
     1381 gctgcaggcg gaggcgcagg cactgaggga aatgattgcg agcatgagga gaaaagcggc
     1441 atgacgaagc gggacacaat tagggacgca tggaagctgc gcgcaccgaa cgaaacgcca
     1501 gcgccgggcc cgcgcggccc ggccatcttc gacaataccg gccaggcgat ccacgtcgct
     1561 ttcacgatca tgtcgcaggc ggcacaacag gacagtgtgt tgcggaagtc cttggtccga
     1621 atcctcggct ccatgaacaa cctcagtccg cagctggagc aatggctcga ccagctgcgc
     1681 ggcacacctt ccagcacggt gaacttcgaa ggcctgtctg ccaatgacgt ccgcgcccag
     1741 tgcgccatga tcctggccgc ggtgcgcacc aaactgccgg agccggagat gtgggcgatc
     1801 cacgcgaagt acgcggcgac cgagcatgag gagaccgggc cgcggctggc cagtggagag
     1861 gtgcgcgccc ggcgctatgc cttctcggca atgaagatcc acgccatcga gcagctggtg
     1921 ggctggcttg tgcagacgtc tgcactgcgc gaggtgccgc tggcggcgat gaagtgcatg
     1981 gtggcgaagt tctactcgaa ccatgcgaag gtggaaatca gcttccgggc cctggcgcag
     2041 gagttcggcg gcaatcacat gctgtatgcc cgaacctaca agcagctggt cgtgctgctg
     2101 cgtccgctgg agatcctggc ggtgcgccgg ctcgaaccct acttcctgca gcagggcatt
     2161 gtgggcgatc agcaggcctg cacggtctga ttgcccttgc gcaaaaaagt gcttgacagg
     2221 gctgttacag gagcccgaga atttgtccaa cctcgaagca ggtacgacta gagaaagccc
     2281 cggatgaaag ttcggggctt tttcttttgg tggccgactc ttgcgcattc atgcgccgca
     2341 ctgtcaccgc acatgacttc cccggcgtgg tggtgcggca ccgttgaagc tgtggggaga
     2401 tcgcacctga cccgagagga tgtagcaact gaatcggccc gccacgtgcg ggcctttgtg
     2461 tttggtgtct ccccgatgta ttgggcatcg gctttgccgg catggctccc ccagtcttgc
     2521 cggctctttt attcgaaggt cctgtggcgt ctcgcgctaa gtcaatctgc cagcatcctg
     2581 gctgtccgcg gctgctcgat gctcccggtc gctgtgaggt gcacgcgcgc cttgtgcagc
     2641 agcagcagga tgagcggcgc ggttcggctc acgatcgcgg gtatgacagc aagtggcgaa
     2701 aagcccgtga tgcttaccta cgcgagcatc cagtctgcga gtgtgacgat tgcaagcggc
     2761 tggactggcc gcctgcgtcc gaggtggtgg accacatcat tccgcatcgc ttgaaggatg
     2821 cgaaggacag tggcaacccg gacgcgatcg cacgcgcaca agagctgttt tggaaccgca
     2881 ggaactggaa agcgatgtca aagcgctgcc acgaccgcaa gaccgcgcgt gaagacggcg
     2941 gctttggacg gtagcaggga ggggcgggtc gaatctctgc gggcctcgcc cggcgaccgc
     3001 gtgttccagc ttttttctgt gcgcgcaagt ttcagggagg gggggtatct gagagaggtg
     3061 cgacatggga cttcgtggac caaaagcaaa accgaccgag ctcaaggtct tggaaggaaa
     3121 ccgtggccat cggcctctga tcactgacgg tcaactgcgc ccgctggtcg agattccgga
     3181 tccgccgaaa cacctgggca aggaagcccg caaggagtgg aagcggatca cggtcgagct
     3241 ggtccggtac aacatcgtgt cacgcctcga ccagcagatg ctgggcatgc tgtgccaggc
     3301 ggtggagcgg gtcgcgctgt tcgagaccgc gctgcagcgg cgcatgctgc agatcatcca
     3361 gcaaaacaag gaacagcctg ctggcaagga acaggatcct gcgcaggctt atgtctcgcg
     3421 gaccccgcag gggtatgaga tgcagtgcat cgagtaccag ctgctgaaca aggagcgcga
     3481 ggtgctgatg aagctcctcg gcgagttcgg gctgtcgccg gccatgcgtg cccgggtggt
     3541 gctcgggcag cctggggcga agcagctgac gcttttcgag ggcggcaagt ccgaccctga
     3601 acccaagaag aacgacggac cgcagagctt tgcggaattc tgatggacta cgtagatcgc
     3661 gctcgccggt acgctgaacg cgtggtggcc ggcgagatta ttgcctgcaa gtgggtccgt
     3721 ctggcctgca agcggcagct ggacgacctg aagcgggaag ggtggaagta tcggttcgac
     3781 ccgtcgaagg cgaaccgggt ctgtcgctac ctggagctgc tgccgcacat caagggcgac
     3841 tgggcgaggc cggtctacgt tgatggcgtc gcggtgttcc cgacgatccg cctcgaagac
     3901 tggcagtgct tcatcgtttg caccgtcttc ggctgggtcg atgagcgggg caagcgtcga
     3961 ttcaagcggg tgtacatcga agtgccccgc aagaatgcca agtcgacgat ctcggccggt
     4021 gttgcgctgt tcatgctgac tgcggacggt gagccgggtg cggagatcta cagcgcggcc
     4081 acgactggcg agcaggcgcg gatcgtcttc gacgatgcaa agcggatggc gctacgctcg
     4141 ccggcgctgc tggagcgctt cggcgtgagc gtgatgacct acgacatcac gatcgcagag
     4201 atggccagca cctttcgtgc cttgaatgcg gaagggtcga cgctcgatgg tctgaacatt
     4261 cattgcgcaa tcgtcgacga gctgcatgcg cacaagcgcc gcgagctgta cgacgtgctg
     4321 gactccggca cgggatcccg gtcgcagccg ctgctgtgga tgatcacgac ggcgggcagc
     4381 gatcgcagtg ggatctgcta cgagcagcgc acgcacgtga cgaaggtcct cgaccaggtg
     4441 tttgaggatg aatcgttctt cgggatcatc ttcacgatcg acgagggcga cgattgggcc
     4501 gacccgtccg tgtggtccaa ggcgaacccg aacttcggcg tgtcggtcct gccggacgac
     4561 atggaagccg cgtgccgcaa ggcgatgtcc atgccgagcg cggtgaacgg tttcctgacc
     4621 aagcgtctga acgtctgggt caacgccgac agcgcctgga tggacatgcg ggcatgggat
     4681 gcctgcggcg atccgtcgct gaagctggag caattcgagg gcgaggaatg cctgatcgcg
     4741 cacgacctcg cctcgaaggt cgacatcgcc gacaaggtgc gcctgttctg gcgtgacatc
     4801 gatggcgagc ggcactacta cgcgtttgca cagcactacc tgaacgagca ggcggtcgag
     4861 gaaggccgga actcgcagta cagcggctgg gcgcgccggg gc
//
LOCUS       DATLGF010000325        17908 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_171090, whole genome shotgun sequence.
ACCESSION   DATLGF010000325 DATLGF010000000
VERSION     DATLGF010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 17908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17908
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171090"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VM406_09910"
     CDS             complement(<1..596)
                     /locus_tag="VM406_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL76321.1"
                     /translation="MSSSIATFLSLRRHPTVAAGALILLVLVAIAVAAPWLTSHDPQE
                     LNPIMRMKGTSEEHVFGTDALGRDIWARVAWGARISLIVGASVAVLSILIGVTIGMVA
                     GYFRRTDAIIMRFMDGLMAIPGVLLAIALMATMRASLTTVIIAIVVPEIPRVVRLVRA
                     LVLTIREQPYVEAALAVGTRVPTILVRHILPNLFAPVMV"
     gene            complement(616..1551)
                     /locus_tag="VM406_09915"
     CDS             complement(616..1551)
                     /locus_tag="VM406_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL76322.1"
                     /translation="MLPILKRILVTIPVMAVVAVIVFMLIHISPGDPAAVMAGDNATA
                     EEVENIRKGMGLDKPLPQQFLIWSGNILTGDLGTSISNHRPVSELILQRVGPTVSIAL
                     WTMVFAVACAIPLGITAAYRAGTWVDRAVMLGSVLAFSVPVFLIGYLFVYAFSIQLGW
                     LPVQGYKPMSEGLVEHFRHLVLPCTTLGLVYMALLTRMTRATMIEALGEDYIRTARAK
                     GLGVPSVIWHALKNAANPIITTIGVGIALLIGGVVVTETVFAIPGIGRLTIDAVLRHD
                     YPVIQGVLLVASGIYVLINLLVDLSYRLFDPRIRY"
     gene            complement(1681..3264)
                     /locus_tag="VM406_09920"
     CDS             complement(1681..3264)
                     /locus_tag="VM406_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL76323.1"
                     /translation="MSMKTRFAGRALFASLVAAAVLSSPLALAQTKTIKMVPTGDLKT
                     LDPHFNTAYLTRNHGYMVFDTLFAQNSKGEIKPQMVDKFTSSKDGLKWTFTLRKGLAF
                     SDGQPVTTKDVIASIQRWAKKDNLGRAMMASVAEMKADSADTFTMTFKEPFGLVLEAL
                     SKPSGSPPFIYPERLAKTDISTPVTEMVGSGPFLFVKEEWVPGNKVVYAKNPKYVPRD
                     EPADGLAGGKVVKIDRVEWTYIPDGNTAAAALRNGEVDMLENVAPDFIRVLESDKAIQ
                     LHTSIGTQGMLIPNSMHPPFNHPKARQALYHIVDQNLYTTALGYPDKYRLQYCGSLYT
                     CNSPTESKAGSDLYRKPDLNKAKQLLAEAGYKGEKVVLLYGTDQALSPAAQVTIQQLK
                     KIGVNLDVQSMDWASVAARRLKKEAPDAGGWSLFHTHGGYFDAGSPAVNPWLSAACGN
                     SLPGWPCDPELDKLRATWMKEVDPAKRRELADQIQLRGFESAPYVMWGQYFPVHATRG
                     LKNTDAMKSGIPVMWNVEK"
     gene            complement(3488..4927)
                     /locus_tag="VM406_09925"
     CDS             complement(3488..4927)
                     /locus_tag="VM406_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HVL76324.1"
                     /translation="MIIFSATLATETNTFGPLPTGLEAFKQRGFYPAGTHPDEMQFFA
                     GPLWAARQRAKGRDWVLKEGLVAAAMPSGITTRHAYETLRDQILEDLRAAMPVDIVLL
                     GLHGAMVADGYDDCEGDLIGRVREIVGKDVIIGVELDPHCHLTRAKTEVADIVVLYKE
                     YPHTDVLDRAYELVDLCVAKFEKRINPAHAVFDCKQIAMMHTTRDPMRGFVDKIKAME
                     GRDGILSISIAHGFPWGDVPDMGTRVLVYTDANVEKAAELARELGQEIIGFRDRLNPG
                     YPDVDEALDQGVAHGEYPVVLADSADNAGGGAPSDSTFVLRRVLERGMRKVAIGPLWD
                     PVAVQICFEAGEGARLPLRIGGKVGPVSGDPVDATWTVHKLRANTFMTGLSDTKAPMG
                     DSALIECDGVYVVINSNRTQAFGTDLFTQFGLDLAQMQIVVVKSSQHFYASYSKIAKK
                     VIYVESPGTVTQDLKSLPFKKIRLPKWPF"
     gene            complement(4955..6079)
                     /locus_tag="VM406_09930"
     CDS             complement(4955..6079)
                     /locus_tag="VM406_09930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVL76325.1"
                     /translation="MHFDFIVVGAGIAGASVAYQLAAKAHVALLEQESQPGYHSTGRS
                     AALFAETYGTPMIRALTCASRAFYVDPPDGFTEHPILTPRGVMHVAQFGQEQLLEDTL
                     TTLLKTSPDVVRLDAKEVLDRCPVLRPEPLLGAAYEHDAMDIDVHALHQGYLKGARAQ
                     GAQLFNDAELESATFADDKWTVRLKDGRELSAHTLVNAAGAWADVVAARSGIAPIGLQ
                     PKRRTAFTFDAPAGHDISTWPAVIGIDESYYFKTDAGQLLGSPANADPVEPHDVQPEE
                     LDVATGIYHIEEVSTLQIRRPKHTWAGLRSFVRDGDLVIGHEPGMPRFFWLAAQGGYG
                     IQSASGAALLAASQLLGEPMPEHLVRFGVQAEALSPARLR"
     gene            6178..6444
                     /locus_tag="VM406_09935"
     CDS             6178..6444
                     /locus_tag="VM406_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76326.1"
                     /translation="MTPDYGQAARNVAWIAHVIGAAGLKRSAIKRLAFYVIATAGQVK
                     AGVFGAFMAKASIESKVRARVQLRIEPRMPSLAGTGLSRAKFPC"
     gene            6544..7512
                     /locus_tag="VM406_09940"
     CDS             6544..7512
                     /locus_tag="VM406_09940"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HVL76327.1"
                     /translation="MNDELAASVELEKYRRIFHAVPDYASFSNLHTGVFLDVNPGFEK
                     MTGYRRGEVIGRQASSINLWVHAAHRAMLVERLPREQALSMETQFRNRAGRILDVEGS
                     FAIFEMENESLLVAVVRDITERKQAAQELAAYRTRLEEQVAQRTSELERAMHRLAELA
                     THDELTGVGNRRDLNARLEKEYKTFKRLGAPCAVAVLDLDNFKAINDQYGHSFGDEVI
                     KIFADILKRALRDVDYVARYGGDEFVLILIGSTPHTAAAAIGRMQQAVQDYGWARHMA
                     GLRVTSSCGIAGFRHDESAEDAFRRADRALYKAKLEGRNRYVVDEV"
     gene            7690..8856
                     /locus_tag="VM406_09945"
     CDS             7690..8856
                     /locus_tag="VM406_09945"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HVL76328.1"
                     /translation="MTQIVVGHTTLVSPTDAGRQLGETISEKFGDQSPDVVIAFVSPK
                     FTYSDVLAAVNHHCRPRILIGCSSAGEFSGCSTGNSTASVMAICSDTILFNAALGKGL
                     RTSRTEAIKQIEHVFTGSQHTDFPYRSALVLTDALAGYAEDLIQDLTLITRGTYQLFG
                     GGAADDARFNETHVFFGTECHSDAVVVLEMLSKKPIGIGVKHGWKTAGKPLRVTEAEG
                     NRIISLNAIPAGDVFAMHAQQTGQVFNRDDPMPFFLHNVIGIESGGEHKLRVPLALNS
                     DGSITCAAEVPLGATVHIMVGNAETACAAAREASEAALAGLNGEKHAGSLLFDCAATR
                     LRLGQDFQDELKAVATTLGSENFAGCNTYGQIARTEGQFSGFHNCTAIVCAIPE"
     gene            8869..10563
                     /locus_tag="VM406_09950"
     CDS             8869..10563
                     /locus_tag="VM406_09950"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL76329.1"
                     /translation="MIPLELVDIFACLQLPESRQDGLKALAAYTRSKEAYLFGKDEEL
                     GIFLPARGLPQTMRHAARWHAIFEQCEARACVRAQLPGPADGEEIDSFVVADKLRLSM
                     LVLLGGEPEPAVVMQLHALLPLIGAKLTVERTAESAAGHAAAARDASRQARSLNLALD
                     ANRRELHKAFEQLEKELAVRREAERKLRETDQKKDEFLAMLAHELRNPLAPITMAAQL
                     IKLPSAGQRQVEQASQIIGRQVAHMTSLLDDLLDVSRVTRGRIVLEKEAVDTKAVIAD
                     AIEQSRSLVQLKQHQLNVRLTGEAVYIEGDKTRLVQIVANILINAAKYTPVQGIIDLR
                     LDAAPEEVFITVSDNGIGIEADVLPHIFELFTQAPRGPDRAHGGLGIGLALVKSLVEL
                     HGGTISAQSQGSNSGSEFTVKLPRYKHSLDASIRSRENQEAASEETLAILVVDDNVDA
                     ASTLATFAEALGHRVLTAYSAKEAAERAASQRFDVIFLDIGLPDMDGYKLAKKIRGLK
                     LCESSVLVALTGYGQPQDRKMAHEAGFDVHLTKPADFSRLMRVLNDSAASRRGAGE"
     gene            11167..11658
                     /locus_tag="VM406_09955"
     CDS             11167..11658
                     /locus_tag="VM406_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HVL76330.1"
                     /translation="MHIWVDADACPAVVKDILFRVAERLHIEVTLVANRLLRVPGSRF
                     IRALQVDSGPDAADQEIVARLAAGDIVITGDIPLASLALDKGGKPLNPRGEWYTRDNI
                     AQQLTMRAFMDDLRASGVDTGGPAAFSHADRQRFANALDRELASRARPQASRGQLQTR
                     ESG"
     gene            complement(11762..12166)
                     /locus_tag="VM406_09960"
     CDS             complement(11762..12166)
                     /locus_tag="VM406_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HVL76331.1"
                     /translation="MSKRDVVFPLGRQALYEKNRYSPAVRSNGFLFVSGQVGSREDGS
                     PEPELEAQVRLAFENLNAILKAAGCSFDDVVDVTLFVVEPESKLETIWKVLGEYWGDA
                     PHPTLTGIGVTWLYGFQFEIKVIAKLPGNANG"
     gene            12311..13237
                     /locus_tag="VM406_09965"
     CDS             12311..13237
                     /locus_tag="VM406_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL76332.1"
                     /translation="MDRFDAMQAFARVVETGSFTKAAQTLHMSKTTVTQLVQQLEARL
                     RVKLLNRTTRRVKVTVDGAAYYERVIRLLADLHDAETSLPNASALPEGRLRVDVPSPL
                     ARMILVPALPAFHEKYPGIQLDLGVSDRKVDLIGENVDCVLRGGEMTDQSLIARRVGD
                     LQLGVYAAPAYLQRAGMPSHPRELEDSHHRIVGFVWSRTGKAYPYAMHRNGESVHVQG
                     RHVLAVDDGNAYLAAGLAGMGIIWLPHYMSKPHVVNGELLPVFEDWRLDPMPLYLAYP
                     PNRHVSVKLRVFIEWVAQLLAVHAPVVARNGG"
     gene            complement(13504..15120)
                     /locus_tag="VM406_09970"
     CDS             complement(13504..15120)
                     /locus_tag="VM406_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL76333.1"
                     /translation="MPTETPKILVVNDNPHSLMALVSLVSSWEQEISCEVITALSGKE
                     ALRQVLLNDFAAILLDVKMPDMDGFETADAIHSRPRSASTPIIFLTAYLSDEFNRFKG
                     YQKGAVDYLFTPVIPEILKAKLTVFVGLARKNIELKLQAALLDKRTQELELINSQLKQ
                     EIDERKIAETRSKTKDEFLAMLSHELRNPLSAISSAAALINLPDVDKSQVQKAGEVIG
                     RQTRHLNHIINDLLDLSRVISGKVELSKRPLDLSRLTETCIETLTLAGRAEKHAISRR
                     LQPAWVDGDPDRLEQIITNLLDNAIKYTPAGGRITVDVRTLDGEVEVVVTDSGIGMSS
                     ELLPHIFDVFVQGEGRPERMRGGLGIGLSLVQQLTSLHCGRIAAHSQGDGKGSTFVLR
                     LPARKEAAPANITGSPLSPSETFRVLVIEDNVDAGEMTSMLLMSCGHQVIRASDGFEG
                     IRKAKVEKPDIALIDIGMPGMDGHEVARRLKEDPATKGIALIALTGYGQPHDKQRALA
                     AGFHTHLTKPADMDRLTEAMTRCISCRGNA"
     gene            15360..>17908
                     /locus_tag="VM406_09975"
     CDS             15360..>17908
                     /locus_tag="VM406_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HVL76334.1"
                     /translation="MNTVADLPLTLDPKQLLLTLQALKKGDFSTRMPSDLVGLDGKIA
                     DTLNEIMEANQEMATSIASVSRVVGHEGSLSQRASIPDAKGSWATIVNSINSLMDDLV
                     RPTTEMARVIGAVAKGDLSQTMLLEVDGRPLRGQFLRTAETANTMVEQLASFASEVTR
                     VAREVGTEGKLGGQANVPGVAGTWKDLTENVNSMAGNLTSQVRNIADVTTAVANGDLT
                     KKITADAKGEILQLKDTINTMVDQLRSFAAEVTRVAREVGTEGKLGGQAHVPGVAGTW
                     KDLTENVNSMAENLTGQVRNIADVTTAVAYGDLSKKITANAKGEILQLKDTINTMVDQ
                     LSSFASEVTRVAREVGTEGKLGGQANVPGVAGTWKDLTDSVNSMAGNLTSQVRNIADV
                     TTAVANGDLTKKITADARGEILQLKDTINTMVGQLRSFAAEVTRVAREVGTEGKLGGQ
                     ANVPGVAGTWKDLTENVNSMAENLTGQVRNIADVTTAVANGDLTKKITADVKGEILQL
                     KNTINTMVDQLNAFASEVTRVAREVGTEGKLGGQAHVPGVAGTWKDLTDSVNSMAENL
                     TGQVRNIADVTTAVANGDLTKKITADAKGEILQLKDTINTMVDQLRSFAAEVTRVARE
                     VGTEGKLGGQANVPGVAGTWKDLTENVNSMAENLTGQVRNIADVTTAVAYGDLSKKIT
                     ANAKGEILQLKDTINTMVDQLNAFASEVTRVAREVGTEGKLGGQANVPGVAGTWKDLT
                     ENVNSMAENLTAQVRGIAGVVTAVAKGDLKKKLTVAAEGEIALLANTINEMTDTLAVF
                     ADQVTSVAREVGVEGKLGGQAKVPGASGTWKDLTENVNQLAANLTTQVRAIA"
BASE COUNT         3631 a         5203 c         5646 g         3428 t
ORIGIN      
        1 accatcaccg gggcaaacag gttcgggagg atgtggcgca ccagaatcgt gggcacgcgg
       61 gtgccgaccg ccagcgcggc ttcgacatac ggctgctcgc ggatggtcag caccaaggcg
      121 cgcaccaggc gcaccacgcg cgggatctcg ggaaccacga tcgcgatgat gaccgtcgtc
      181 aggcttgcgc gcatggtcgc catcagcgcg atcgccagca gcacgccggg gatcgccatc
      241 aggccgtcca tgaagcgcat gatgatggcg tcggtacggc ggaagtagcc ggcgaccatg
      301 ccgatggtga cgccgatgag gatcgacagc accgcgacgg acgcgccgac gatcagcgaa
      361 atccgcgcac cccatgcgac gcgcgcccag atgtcgcgcc cgagggcatc ggtgccgaac
      421 acatgctctt cggaggtccc cttcatgcgc atgatcgggt tgagttcctg cgggtcgtgg
      481 gaggtgagcc acggcgcggc aaccgcgatc gcgaccagga ccagcaggat gagggcgccg
      541 gcagccaccg tcgggtgccg gcgcagcgag aggaatgtgg cgatggacga agacacgctt
      601 ggtactccgg gcagatcagt aacggatgcg gggatcgaac aaccggtagg acaggtcgac
      661 cagcaggttg atcaggacgt agatgccgga ggcgaccagc agcacgccct ggatgacggg
      721 atagtcgtgg cgcagcacgg catcgatggt gagccggccg atgccgggaa tcgcgaacac
      781 ggtttcggtc acgaccacgc cgccgatcaa cagcgcaatg ccgacaccga tggtggtgat
      841 gatcgggttg gccgcgttct tcagcgcatg ccagatcacg gacggcacgc ccaggccctt
      901 ggcgcgggcg gtgcggatgt agtcctcgcc gagtgcctcg atcatcgtcg cgcgcgtcat
      961 gcgggtgagc agcgccatgt agacgagacc aagcgtggtg cagggcagga cgagatgccg
     1021 gaaatgttcg accagccctt cggacatcgg cttgtatccc tgcaccggca gccagccgag
     1081 ctggatcgaa aacgcataga cgaacaggta gccgatcagg aacaccggca ccgaaaacgc
     1141 gagcaccgag ccgagcatga ccgcgcggtc gacccaggtg ccggcgcggt aggcggcggt
     1201 gatgccgagc gggatcgcgc aggcgacggc gaagaccatc gtccacagtg cgatcgatac
     1261 cgtcgggccg acgcgctgca ggatcagttc cgataccggg cggtggttgc tgatcgaggt
     1321 gccgaggtcg ccggtcagga tgttgccgga ccagatcagg aactgctgcg gcaggggttt
     1381 gtcgagcccc atgcccttgc ggatgttctc gacttcctct gccgtggcgt tgtcgccggc
     1441 catcacggcg gcgggatcgc ccggcgagat gtggatcagc atgaagacga tcacggcgac
     1501 aacggccatg accgggattg tgacgaggat gcgcttgagg atgggtagca tcgggcgagg
     1561 gtcggaaaca ggctggggcc tggagtttga aacagagagc ttgcgggtcg cacgaccagg
     1621 aactggttcc ctcccccttg cagggggagg gaatacgaca gagggcggaa tcagcgtgga
     1681 ttacttctcc acattccaca tcaccggaat ccccgacttc atcgcatccg tgttcttcag
     1741 accacgggtc gcgtgaaccg ggaagtactg gccccacatc acgtacggcg ccgactcgaa
     1801 gccgcgcagc tggatctggt cagccagttc gcggcgcttg gccggatcga cttccttcat
     1861 ccaggtcgcg cgcagcttgt ccagttccgg atcgcacggc cagcccggca ggctgttgcc
     1921 gcaggcggcc gacagccacg ggttgacggc cgggctgccc gcgtcgaagt agccgccgtg
     1981 ggtgtggaac aggctccagc cgccggcgtc cggcgcttcc ttcttcaggc ggcgcgcggc
     2041 aaccgaagcc cagtccatcg actgcacgtc cagattgacg ccgatcttct tcagttgctg
     2101 gatcgtcacc tgtgccgccg gcgacagcgc ctggtcggtg ccgtacagca gcacgacctt
     2161 ctcgcccttg tagcctgctt cggccagcag ctgcttggcc ttgttcaggt ccggcttgcg
     2221 gtacaggtcg gaaccggcct tgctctcggt cggcgagttg caggtgtaga gcgaaccgca
     2281 gtactgcagg cgatacttgt ccggatagcc gagcgcggtc gtgtacaggt tctggtcgac
     2341 gatgtggtac agcgcctggc gtgccttcgg gtggttgaac ggcgggtgca tcgagttggg
     2401 gatcagcatg ccctgcgtgc cgatcgacgt gtgcagctgg atcgccttgt cggattcgag
     2461 cacccggatg aagtcgggcg caacgttttc cagcatgtcg acttcgccgt tgcgcagcgc
     2521 ggccgcggcg gtgttgccat ccgggatgta ggtccattcg acgcggtcga tcttcacgac
     2581 tttgccgccg gccaggccat cggccggctc atcgcgcggc acgtacttcg ggttcttcgc
     2641 gtacacgacc ttgttgccgg gcacccattc ttccttcacg aacaggaagg ggccggagcc
     2701 gaccatctcg gtgaccggcg tgctgatgtc ggtcttggcc aggcgttccg gatagatgaa
     2761 cggcgggctg cccgacggct tggacagcgc ctcgagcacc aggccgaagg gctccttgaa
     2821 ggtcatcgtg aaggtgtcgg cggaatcggc cttcatctcg gcaaccgacg ccatcattgc
     2881 gcggccgagg ttgtccttct tggcccagcg ctggatcgag gcgattacgt ccttggtcgt
     2941 gaccggctga ccgtcggaga acgccagtcc tttgcgcagc gtgaaggtcc acttcaggcc
     3001 gtccttgctg gacgtgaact tatcgaccat ctgtggtttg atctcgccct tgctgttctg
     3061 tgcgaacagc gtgtcgaaca ccatgtagcc gtggttgcgg gtcaggtagg cggtgttgaa
     3121 gtgcgggtcg agcgtcttca ggtcgccggt cggcaccatc ttgatggtct tggtctgcgc
     3181 cagcgcgagc ggcgaggaca gcacggcggc ggcgacgagc gacgcgaaca gggcccggcc
     3241 tgcgaatctt gttttcattg acatctccag tgggggtgaa atggattgaa ggattttttg
     3301 cccgtggctc ttgctggtcc ggatgaacga tcagacggcg tcacaaagtc ctctgtcatt
     3361 ccgaacgcag tgaggaatct ccggccgcag caattgcacg aacggtggag acccctcact
     3421 ggcgttcggg atgacaggga taggttttgc tgtctggttc cgcatgttca tctggacgct
     3481 ctctgaacta gaacggccac ttcggcagac ggattttctt gaacggcagg gacttcagat
     3541 cctgcgtcac ggtgcccggc gattcgacat agatcacctt cttcgcgatc ttcgaatacg
     3601 acgcatagaa atgctgcgat gacttcacga ccacgatctg catctgcgcg agatcgaggc
     3661 cgaactgggt gaacaggtcg gtgccgaagg cttgcgtgcg gttcgagttg atgacgacat
     3721 acacgccgtc gcattcgatc agtgcgctgt cgcccattgg cgccttggta tcggacaggc
     3781 cagtcatgaa ggtgttggcg cgcagcttgt gcacggtcca ggtcgcatcg accgggtcgc
     3841 cggataccgg accgaccttg ccgccgatgc gcagcggcag gcgggcgcct tcgccggctt
     3901 cgaagcagat ctgtaccgcg accgggtccc acagcggacc gatcgcgacc ttgcgcatgc
     3961 cgcgctcgag cacgcggcgc agcacgaagg tggaatcgct cggtgcgccg ccaccggcat
     4021 tgtcggccga atcggccagc accaccggat actcgccgtg ggcaacgccc tgatccagcg
     4081 cttcgtccac gtcgggatag ccggggttaa ggcggtcgcg aaagccgatg atctcctggc
     4141 cgagttcgcg tgccagttcg gcagccttct ccacgttggc gtcggtataa accagcacgc
     4201 gcgtacccat gtccggcaca tcgccccacg ggaagccgtg cgcgatcgag atcgacagga
     4261 tgccgtcgcg gccttccatc gccttgatct tgtcgacgaa gccgcgcatc ggatcgcgcg
     4321 tggtatgcat catcgcgatc tgcttgcagt cgaacaccgc gtgcgcgggg ttgatgcgct
     4381 tctcgaactt cgccacgcac aggtcgacca gttcgtaggc gcggtcgagc acgtcggtat
     4441 gcgggtactc cttgtacagc acgacgatgt ctgccacttc ggtcttggca cgcgtgaggt
     4501 ggcagtgcgg atcgagctcg acgccgatga tcacgtcctt gccgacgatc tcgcgcacgc
     4561 gcccgatcag gtcgccttcg caatcgtcgt agccgtcggc caccatcgcg ccgtgcaggc
     4621 cgagcagcac gatatccacc ggcatcgccg cgcgcaggtc ttccaggatc tggtcgcgca
     4681 gggtttcata ggcatggcgc gtcgtgatcc cgctcggcat cgcggccgcg accagccctt
     4741 ccttcagcac ccagtcgcgc cccttggcgc gttgacgcgc cgcccacagc gggcccgcga
     4801 agaactgcat ctcgtccgga tgcgtgccgg ccgggtagaa gccgcgctgc ttgaaggctt
     4861 caaggccggt cggcagcggg ccgaaggtat tggtttcggt ggcaagcgtg gcggaaaaga
     4921 tgatcacggg cgctccttga acggaaagaa ggggtcagcg cagacgcgcc ggcgacagcg
     4981 cttcggcctg cacgccgaag cgaaccaggt gttccggcat cggttcgccg agcagttgcg
     5041 aagcggccag cagcgccgcg ccggacgcgc tctggatgcc atagccgccc tgtgcggcca
     5101 gccagaagaa gcgcggcatg ccgggttcgt gcccgatcac caggtcgcca tcgcgcacga
     5161 acgagcgcag cccggcccag gtgtgcttcg ggcggcggat ctgcagcgtg ctcacctctt
     5221 cgatgtgata gatgccggtg gccacgtcca gctcttccgg ctgcacgtcg tgcggctcga
     5281 ccggatcggc attggcgggc gaaccgagca gctggccggc atcggtcttg aagtagtacg
     5341 actcgtcgat gccgatcacg gccggccagg tcgagatgtc gtggccggcg ggagcatcga
     5401 aggtaaaggc ggtgcggcgc ttcggctgca ggccgatcgg cgcgatgcct gaacgggctg
     5461 ccaccacatc ggcccaggcg cctgcggcgt tgacaagcgt atgtgcgctc agctcacgcc
     5521 cgtccttcag gcgcaccgtc cacttgtcat cggcaaaggt cgcgctttcc agttcggcat
     5581 cgttgaacag ttgcgcgccc tgggcccgcg cgcccttcag gtagccctgg tgcagcgcat
     5641 gcacgtcgat gtccatcgcg tcgtgctcgt aggcggcgcc gagcagcggc tccggacgca
     5701 gtaccgggca acggtcgagc acttccttcg catccaggcg taccacgtcg ggtgacgtct
     5761 taagcagtgt ggtcagtgtg tcttcgagca gctgctcctg cccgaactgt gccacgtgca
     5821 tcaccccacg cggcgtgagg atgggatgct cggtgaaacc gtccggcgga tcgacgtaga
     5881 aggcgcggct cgcgcaagtg agcgcgcgga tcatcggcgt gccgtaggtc tccgcaaaga
     5941 gcgcggccga gcggccggtc gagtgatagc cgggctggga ttcctgctcc agcaaggcca
     6001 cgtgggcctt ggcagccagc tgataggcga cggaagcgcc ggcgatgccg gcgccgacca
     6061 cgatgaaatc gaaatgcacg gggtttccct aaatttttct cttacgagaa ttctcggata
     6121 ggagaatact gcggcgggac tttgcgtgtt gtcaagggaa aagattattc gggaggaatg
     6181 acgcctgact atggccaggc tgcccggaat gtcgcttgga tcgcgcatgt cattggcgct
     6241 gcggggctga aacggagtgc gatcaagcgc ctggctttct atgtcattgc gacggccggc
     6301 caggtcaagg ccggtgtctt cggtgcgttc atggcaaagg caagtatcga gagcaaggta
     6361 cgggcgcgag tgcagttgcg cattgaaccg cgcatgccgt cgctggcagg cacaggccta
     6421 tctcgcgcca aatttccatg ttagattggc ctcaccggtg tttccgtggg gaaagtcaca
     6481 gagagaacct cgctgcctgg gtgcggtgcg cccgccgcag cagcgatgtt cggagcagag
     6541 accatgaacg atgaattggc tgccagcgta gagctcgaga agtaccgccg gatctttcat
     6601 gcggtgccgg actatgcatc gttctccaac ctgcacaccg gggttttcct cgacgtgaat
     6661 ccggggttcg agaaaatgac cggctaccgg cgcggagaag tgatcggccg ccaggcgtcc
     6721 agcatcaatc tctgggtaca cgccgcgcat cgcgccatgc tcgtcgaacg gcttccgcgc
     6781 gaacaggcgt tgtcgatgga aacccagttc cgaaaccggg ccggcaggat cctcgatgtc
     6841 gagggatcgt tcgcaatctt cgagatggag aacgaaagcc tgctggtggc cgtggttcgt
     6901 gacatcaccg aacgcaagca ggcggcgcag gaactcgccg cctaccgaac ccggctcgag
     6961 gaacaggtcg cgcaacgcac atcagagctc gaacgggcca tgcacaggct cgcggagctt
     7021 gcaacgcatg acgagctgac gggcgtcggc aaccgccggg acctcaatgc acggctggaa
     7081 aaggagtaca agaccttcaa gcggctcggc gcgccatgtg cggtggctgt tctcgacctg
     7141 gacaacttca aggcgatcaa tgaccaatac ggccacagct tcggggatga agtgatcaag
     7201 atcttcgccg acatcctcaa acgcgcgttg cgggatgtcg actatgtggc gcgatacggc
     7261 ggagatgagt ttgtcctgat cctgatcggt tcgacgccgc acaccgcagc cgctgcaatc
     7321 ggccgcatgc agcaagccgt ccaggactat ggctgggccc ggcacatggc agggctgcgc
     7381 gtcacctcgt catgtggcat cgcgggtttt cggcatgacg aaagcgccga agatgctttc
     7441 cgacgcgcgg accgtgccct gtacaaggcc aagcttgaag gcaggaaccg ctacgtcgtg
     7501 gacgaggtgt agcggttcgg cgcacttcct tttggctgca tgcaagcttg gttgtaccgt
     7561 ccttttttag tcgccaagtt gtttgaggtt tctcaaattg agtcccggta atttcacgta
     7621 ggcggaagta tggacgtatg attgcgggcg ctttttgcat ccacagtagt gctaatcggc
     7681 accagacaca tgactcaaat tgtcgttgga cacaccacct tggtttcgcc caccgacgcc
     7741 ggtcggcagc tcggggaaac aatttctgaa aaatttggcg accaaagccc agacgtggtt
     7801 atcgcttttg tctcgcccaa atttacttac tcagacgtct tagctgcggt caatcaccat
     7861 tgccggcccc ggattctgat cggctgctcg tccgcaggcg agttttccgg ttgctcgaca
     7921 gggaattcca cggcatccgt gatggcaatc tgctcggata ccatactttt caatgccgca
     7981 ctcgggaaag gattgcgtac ctctagaacc gaggcgatta aacaaatcga gcacgttttt
     8041 accgggagcc aacacaccga ctttccttat cgtagcgcat tggtgctaac ggacgctctc
     8101 gcgggatatg ctgaagatct gattcaggat ctcactctga ttacgcgcgg cacctaccag
     8161 ttatttgggg gcggcgcggc tgatgacgcg aggttcaacg agacccatgt attttttggt
     8221 accgaatgcc attcggacgc tgtcgtcgtg ctcgaaatgc tctcgaagaa gcctatcgga
     8281 attggcgtta aacacggatg gaaaaccgcc ggcaaacctt tgcgcgttac cgaagccgaa
     8341 ggcaaccgga ttatcagtct gaacgccatt ccggcgggtg acgtgttcgc catgcatgca
     8401 cagcaaactg gccaagtatt caaccgcgat gaccccatgc cgtttttcct gcataacgtt
     8461 attggaatcg agtcgggcgg tgagcacaaa ctcagggtgc cgcttgctct aaattcggat
     8521 gggtccatca cttgcgctgc cgaagttccg cttggcgcca ctgtacatat catggttggt
     8581 aatgcagaaa ctgcatgtgc ggcggcacgc gaagcctccg aggctgcact ggcggggtta
     8641 aatggagaaa aacatgccgg ctcgctcctg ttcgattgtg ccgcgacgcg tctgcggttg
     8701 gggcaagact ttcaagacga attgaaagcc gttgctacta cactcggctc cgaaaacttt
     8761 gccggctgca atacatatgg acagattgcg cgtaccgaag gtcagtttag cggttttcac
     8821 aattgcaccg cgatcgtgtg cgcgatccct gagtagctat ttttggagat gattccttta
     8881 gaacttgtag acatattcgc atgtctgcag ttgccagaat cccgccagga cggcctcaag
     8941 gcgctggctg cgtacacacg gtccaaggaa gcataccttt tcgggaaaga tgaagaactt
     9001 ggcatttttt tgccggcgcg aggattgcct caaacgatgc gtcatgcggc aagatggcat
     9061 gcgattttcg agcagtgcga agcgagggcc tgtgtgcgcg cgcaattgcc gggacccgct
     9121 gatggtgaag aaatcgatag tttcgttgta gctgacaagc tccggctgtc catgcttgta
     9181 ttgcttggtg gcgaacccga accggctgtc gtgatgcagc tacacgcgct tcttccactg
     9241 ataggggcaa agctgaccgt tgaacgtaca gcggaatcgg cggccggcca tgccgccgcc
     9301 gcccgcgacg ccagcaggca agccagatca ctaaaccttg cactcgatgc caaccgcagg
     9361 gaactgcata aagcatttga gcaactagaa aaagagctcg ctgtgcgccg agaggcagaa
     9421 cgcaagctgc gcgaaactga ccagaagaaa gatgaatttc tcgcgatgct cgcgcacgaa
     9481 ctacgcaacc cgctagcgcc tatcactatg gcagcgcaac tcatcaagct tccttcggca
     9541 ggccagcgcc aggtggaaca agcgagccaa attatcggcc ggcaggtggc gcatatgacc
     9601 agtctgcttg acgatctctt ggacgtgtcg cgcgtcacgc gtggcaggat tgttctggaa
     9661 aaagaggccg tcgataccaa agccgtaatt gccgacgcga ttgaacaatc gaggtcgttg
     9721 gttcagctga agcagcatca gctcaatgtc cgtttgacag gagaggcggt gtacattgag
     9781 ggagacaaaa cgcgccttgt gcagattgtt gcgaacatcc tgattaatgc cgccaaatac
     9841 acgcctgtcc agggcatcat tgacctgaga ctcgatgcag cgcctgaaga ggtttttatc
     9901 acggtaagcg acaatggcat tgggattgaa gccgacgtgt tgcctcacat tttcgagttg
     9961 ttcacgcaag cacctcgagg cccggatcgc gcacatgggg ggctcggtat tggactcgcg
    10021 ctggtcaaga gcttggtaga actacatgga gggacgataa gcgcacaaag ccaagggtct
    10081 aattcaggga gcgaattcac ggtaaaactt ccgcggtata aacactctct ggacgcgtcg
    10141 attcgcagca gagagaatca ggaagctgct tctgaagaga cattggcgat actcgtggtt
    10201 gacgataatg tcgatgccgc aagcactctc gcgacatttg ccgaagcgct cgggcatcgt
    10261 gtgcttactg cgtactctgc taaagaggca gctgagcggg cggcatccca gaggttcgac
    10321 gtaatattct tggacattgg tctgccggat atggacggat acaagctggc aaaaaagata
    10381 aggggactaa agttgtgtga aagttcggta cttgttgcat tgaccggata cggccaaccg
    10441 caggacagga aaatggcgca tgaagccgga tttgacgtcc atttgacaaa accggcggac
    10501 ttttcacgac tcatgagagt gctgaatgac tcagccgcaa gtcgacgtgg tgccggtgaa
    10561 tgattgacgt caaaaggcga aggcttcggc ccaatacata ttcaccttat agcgttatct
    10621 gctttactcg acttactcct gccttcaggc gcttcaattt gcagcaaggg aatttctttc
    10681 gttgcagttc gcatgttagg aaaagacatg ctgccatgca cgaccatcat caaagataca
    10741 ggggtgcgat tggcatggaa agttcggaag cgtcaggaac gtgcggaagt gagtgtctat
    10801 gaggccttcc ctaattgccc tcaaacgtcc gcattgggtc gacatcggcc tttccgaagc
    10861 tgcataagtg agcgcaaatg gctgagaagt tcatgccgcg cttgtctcgc cgagtcttgt
    10921 ccgctaccgc ttttcattgg cgcaacgtct tcacctcttt tgcgggatga aggcagcgtc
    10981 gacaatgaat ctgcagatga attgggatac cggcaccttc gttcaaaccg cggtcttgtg
    11041 gcggcactgt tcgggatttg cttttgatcc aagagcgttg ccctgaagcg ggtctgggac
    11101 gatgcgcacc agtcaggccg gctgggatac actcgtggcc cctgtcacac gctccactcc
    11161 actgtcatgc atatctgggt cgacgccgac gcctgccccg ccgtggtcaa ggacattcta
    11221 ttcagagtgg ccgagcgatt gcacatcgag gtgacgctgg ttgcgaaccg gttgctgcgg
    11281 gtgccgggtt cgcgcttcat tcgggcattg caggtggatt ccggcccgga tgccgctgac
    11341 caggaaatcg tcgcgcgcct ggctgcgggc gacatcgtca tcaccggcga tattccgctc
    11401 gccagccttg cgctggacaa gggcggcaag cccttgaacc cgcgcggcga gtggtacacc
    11461 agggacaaca ttgcacagca actgacgatg cgcgccttta tggatgacct gcgtgcatcc
    11521 ggcgtcgata ccggcgggcc ggccgcattc agccatgctg atcgccagcg gtttgccaat
    11581 gcgctggacc gcgaactcgc gagcagagcg cgcccgcaag catcgcgcgg gcaactgcaa
    11641 acgcgggaat cggggtaagg ggagaccagc atcgcgaacg taagccgacc agggtacgcc
    11701 cggagcgagc gcactttcct catgcgatgc gtgcgaaaac gacctggccg tcgacggtgc
    11761 gtcagccgtt ggcgttcccg ggcagcttcg cgatcacctt gatctcgaac tggaagccgt
    11821 agagccaggt cacgccgatt cctgtcagcg tcgggtgcgg tgcgtcgccc cagtattcgc
    11881 ccagcacctt ccagatcgtt tcgagcttgg attcgggctc gacgacaaag agggtgacgt
    11941 ccaccacgtc gtcaaagctg caaccggcgg ccttcagaat cgcgttgaga ttctcgaacg
    12001 ccagcctgac ctgtgcttcc agttccggct ccggcgaacc atcttcgcgg ctgccgacct
    12061 gtccggatac gaacaggaaa ccgttggaac gaaccgccgg cgaataacgg ttcttttcat
    12121 atagcgcctg gcgtccaagc gggaacacga catcacgttt gctcattgct cacctctcca
    12181 taataggggc ggcgccccgg gttcagtccg acctactcta cgcgcggatc cgaccgggat
    12241 aaacgagcaa gtccatccat cactgtttgt acaatccaaa taatcacggg ccacgaagca
    12301 ggcagaaaac atggacagat tcgatgcaat gcaggcattt gcccgagtgg tggaaaccgg
    12361 cagcttcacc aaggcagcgc agacgctgca catgagcaag accacggtga cgcaactggt
    12421 gcagcagctg gaggcgcggc tgcgcgtgaa gctgctgaac cggaccacgc gcagggtaaa
    12481 ggttacggtc gacggcgcgg cctactacga gcgcgtcatc cgcctgctgg ccgatctgca
    12541 cgatgcggaa accagcctgc cgaacgcatc ggcattgccg gaaggccgac tgcgcgtgga
    12601 cgtccccagc ccgctggcac gcatgatcct ggtgccggca ctgcccgcat ttcacgagaa
    12661 atatcccggc atccagctcg acctgggcgt gagcgaccgc aaggtggacc tgatcggcga
    12721 gaacgtggat tgcgtgctgc gtggcggcga gatgacagac cagtcgctga tcgcgcgccg
    12781 ggtgggcgac ctgcagcttg gcgtctacgc cgcgcccgcc tatctgcaac gcgccggcat
    12841 gccctcgcac ccgcgcgagc tggaagactc gcaccaccgc atcgtcggct tcgtctggtc
    12901 gcgcaccgga aaggcttatc cgtatgcgat gcacaggaac ggcgaaagcg ttcatgtaca
    12961 gggccgccat gtgttggcgg tcgacgacgg caatgcctat ctcgcagcgg gcttggccgg
    13021 catgggcatc atttggctgc cgcactacat gagcaagcca cacgttgtga acggcgaact
    13081 gctaccggtg ttcgaagact ggcgtctcga ccccatgccc ctgtacctgg catatccgcc
    13141 gaaccggcat gtcagcgtca agctgcgcgt gttcatcgaa tgggttgcgc agctgctggc
    13201 ggtgcatgcg cctgtcgttg cgaggaatgg cggatgacag ttccacaagc gagggagctg
    13261 cggtcagcct gcctctctac gaaactgcgc ctgcctgggg aaaaatcctg gccggaagag
    13321 agcgaaacga tcggcatccc tgccggcgcg gcggtcgcgt cggtccggcg tcccctggat
    13381 ttccccgaca ggcgatgaac aatccagcag aggtcggaca cacgtcgccc tttggccgac
    13441 atttcttgat caggggatcc aggtcctggg gttaccgcgc aaagcttgag ccagccgcga
    13501 cgcttacgcg tttccgcggc agctaatgca tctcgtcatg gcttccgtaa gccgatccat
    13561 atcagcgggt tttgtgagat gggtatggaa accggcggcc aatgcacgtt gcttgtcgtg
    13621 cggctgcccg tagccggtga gcgcaatgag agcaattccc ttcgtggcgg ggtcttcttt
    13681 caaccgcctg gcaacttcat gtccgtccat tcccggcatg ccaatatcga tgagggcgat
    13741 gtcgggtttc tcgaccttcg ctttgcggat cccctcaaag ccgtccgacg ccctgatgac
    13801 ctggtggccg caggacatca gcagcatgga cgtcatctcc ccggcatcga cgttgtcttc
    13861 aattaccagc acccgaaacg tttccgaggg tgagagagga ctgccagtga tgttcgccgg
    13921 cgccgcttcc tttcgtgcag gcagacgtag cacgaaggtg ctgcctttcc cgtcgccttg
    13981 gctatgcgcg gcgatcctcc cgcaatgcag tgaggtcagt tgctgaacga gggaaagtcc
    14041 aatccccagt ccaccccgca tccgctcggg ccttccctct ccttgcacaa acacgtcgaa
    14101 gatatgaggg agcagctccg aagacatgcc gatccccgaa tcggtcacga cgacttcgac
    14161 ctctccgtcc agagtacgga catcaacggt gattcttcct cccgcaggcg tgtacttgat
    14221 tgcgttgtcc agcaagttgg tgatgatctg ctcaagacga tccgggtcgc cgtcgaccca
    14281 cgcgggctgc aaccgccgtg agatcgcgtg cttctccgca cgtcccgcca aggtgagcgt
    14341 ttcgatacac gtttccgtca accgggaaag atccagtgga cgcttactca gctcgacctt
    14401 gcccgagatt acccggctca agtcgaggag gtcattgata atgtggttca ggtgtctcgt
    14461 ctggcggccg atgacttcac ctgccttctg tacctggctc ttgtccacgt caggaagatt
    14521 gatgagcgcc gcggcgctgg atatcgcgct cagcggatta cgcagctcgt gactcaacat
    14581 cgccaggaac tcgtctttgg tcttgctccg tgtctccgcg atcttcctct catcaatctc
    14641 ttgcttgagc tgcgagttga tcagctcaag ctcttgcgtg cgtttatcaa gaagcgctgc
    14701 ttgcagcttg agttcaatgt tctttcgagc cagaccgaca aatacggtca gttttgcttt
    14761 cagaatttcc ggaataacgg gtgtaaatag ataatcgacg gcgcctttct gataaccctt
    14821 gaaccgattg aattcgtcgg acagatatgc cgtcaggaag atgatgggag tggatgctga
    14881 gcgggggcgg gaatgaatgg cgtccgcggt ctcgaaacca tccatgtccg gcatcttcac
    14941 gtccagcagt atcgcggcga agtcattcaa caagacctgg cgcaacgcct cttttccgga
    15001 gagcgctgtt attacctcgc aggatatttc ttgctcccag gaagagacca ggcttacaag
    15061 agccatcagg ctgtgcgggt tatcgttgac gacgagaatc ttcggtgttt ctgttggcac
    15121 aatgcgcctc ggctcgtttt gcaggatgtc gcaggcgaaa agagcgacag cacggtggtt
    15181 gagaacagct gcgcgactat attgccagta gttaacgccg gcgtgcgggg agggaggggt
    15241 tgatatttgt caacgctcgt tcttgtgcgc gtgcgaccac aggggttccc gccataattc
    15301 tatccctctg gaaacagaac cagttccttt cccggccttt tctcctcgcg agccccatga
    15361 tgaatacagt cgccgacctg ccgctcactc tcgatccgaa acagcttctg ctgacgctac
    15421 aagcgctgaa aaaaggcgat ttctcgacgc gcatgccgtc cgatctggtg gggctggacg
    15481 ggaaaattgc cgatacgctc aatgagatca tggaggccaa ccaggaaatg gccaccagca
    15541 tcgcatcggt gagccgggtg gtcggacacg aaggtagcct gagccagcgc gccagcattc
    15601 cggacgcgaa ggggagttgg gcgacgattg tcaattccat aaacagcctc atggacgatc
    15661 tcgtgcggcc tacgaccgaa atggcgcgcg tcatcggcgc ggtcgcaaaa ggagatttgt
    15721 cgcagaccat gttgctggag gtcgacggca ggccgttgag aggccagttt ttacggaccg
    15781 ccgaaacggc caacacgatg gttgaacaac tggcgtcctt cgcctccgag gtcacgcgcg
    15841 tggcgcgcga ggtcggtacc gaagggaagc tgggcggtca ggcgaacgtg ccgggcgtgg
    15901 cgggtacgtg gaaggacctt accgagaacg tcaattcgat ggcgggaaat ctgacctcgc
    15961 aggtgcgaaa catcgccgac gtgacaacag ccgtggcgaa cggcgacctg accaagaaga
    16021 tcaccgccga cgccaagggc gaaatcctgc agttgaagga caccatcaac acgatggtgg
    16081 accagctgcg ctcctttgcc gcagaagtga cccgcgtggc gcgggaggtc ggcaccgagg
    16141 gcaagctggg cgggcaggcg catgtacccg gtgtggcggg cacctggaag gatttgacgg
    16201 aaaacgtgaa ttcgatggcg gagaacctga ccggccaggt gcgaaacatc gctgacgtta
    16261 caaccgccgt ggcttatggc gatttgtcga agaagattac ggcgaatgca aagggcgaga
    16321 tcctgcaact gaaagacacc atcaacacca tggtggacca gctttcctcg ttcgcctccg
    16381 aggtaacccg cgtagcgcgt gaagtgggca ccgaaggcaa gctggggggg caggccaatg
    16441 taccgggcgt cgcgggtacc tggaaagact tgaccgattc ggtcaactcg atggcaggca
    16501 acctgacctc gcaggtacga aacatcgcgg acgtgacgac tgcggtggcc aatggcgacc
    16561 tgaccaagaa gattaccgcc gatgcgcgcg gcgaaatcct tcagctgaag gatacgatca
    16621 acacgatggt gggtcagttg cgttcattcg ccgcggaagt gacccgcgtc gcacgggaag
    16681 tcggaacaga gggcaagctc ggcggacagg cgaacgtgcc gggtgtcgcg ggcacctgga
    16741 aagacttgac cgagaacgtg aactccatgg cggagaacct gacaggacag gtgcgaaaca
    16801 ttgcggacgt gacgacggcc gtggcgaacg gcgaccttac aaaaaagatc acggctgacg
    16861 tcaagggcga gatcctgcag ctgaagaaca ccatcaacac catggtggac cagttgaatg
    16921 ccttcgcttc cgaagtgaca cgggtggccc gcgaagtggg gaccgagggc aagctgggtg
    16981 gtcaagccca tgtccccggc gtggccggca cctggaaaga cctgactgac tcggtgaact
    17041 cgatggccga gaacctgacg ggccaggtgc gaaacattgc ggacgtgacg acggcggtcg
    17101 ccaatggcga cctcaccaag aagatcaccg ccgacgcaaa gggcgagatc ctgcagttga
    17161 aggacacgat caacacgatg gtggaccagc tgcgatcctt cgcggccgag gtaacccgcg
    17221 tcgcgcgtga agtgggcacg gaaggcaagc tgggcggcca ggcaaatgtg ccgggcgtcg
    17281 ccggtacctg gaaagacctg accgagaacg tcaactccat ggcggaaaac ctgaccggcc
    17341 aggtgcgaaa catcgctgac gtcacgaccg ccgtggcata tggtgatctg tcgaagaaga
    17401 ttacggccaa cgcaaaaggc gagattctgc agctgaagga caccatcaat accatggtgg
    17461 accagttgaa tgccttcgct tccgaagtga cacgggtggc ccgcgaagtg gggaccgagg
    17521 gcaagctcgg tggacaagcc aacgtgccgg gagtagccgg cacatggaaa gatctgacgg
    17581 agaacgtcaa ctccatggcc gagaatctca ccgcgcaggt gcgtggtatt gccggcgtgg
    17641 tcaccgccgt cgccaaaggc gatttgaaga agaagctgac ggtggcagca gagggcgaga
    17701 tcgccttgct tgcgaatacg atcaacgaga tgacggatac cctggccgtg ttcgccgatc
    17761 aggtgacatc ggtcgcgcgc gaagtcggcg tggaaggaaa actcggtgga caggcgaagg
    17821 tgcctggtgc ttccggcacc tggaaggact tgaccgagaa cgtcaatcag ctggccgcaa
    17881 acctgacgac acaggtgcgc gccattgc
//
LOCUS       DATLGF010000326         5838 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_139514, whole genome shotgun sequence.
ACCESSION   DATLGF010000326 DATLGF010000000
VERSION     DATLGF010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139514"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="VM406_09980"
     CDS             <1..1149
                     /locus_tag="VM406_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL76335.1"
                     /translation="FRLVSCNESVERILQHDFDDCIGKPLTTIPGLEPFAESVMGAFS
                     EQSARAAGEGGVAHWQKQIEVPRRTGGPSEVDNNITLLTRGSYLPVSGDVGHIVVFDD
                     ITEVISAQRSIAWAEVARRLAHEIKNPLTPIQLSAERLQMKLETKLEPADKAVLSKGT
                     GTIVSQVSAMKRMVDDFRDYAKTPAAVLVPLDLNALVEEILQLYMADIERGVIHPRLT
                     PELPLVMGDATQLRQVIHNLLQNALDAVGDADNDAPFIEVLTETVHYQHGDGSRRTAV
                     RLCVTDNGPGFSPKILARACEPYVTNKPRGTGLGLAVVKKIVEEHGGRIDIRNRSTGS
                     GAKVAILLMKLSGGQAPEATSADARQAARAEEHGHGAGIPPGTAGIET"
     gene            1162..1860
                     /locus_tag="VM406_09985"
     CDS             1162..1860
                     /locus_tag="VM406_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL76336.1"
                     /translation="MANILVVDDEMGIRELLTEILGDEGHAVTAAENAQEAREQRARE
                     QPDLVLLDIWMPDTDGVTLLKEWQRDGLLTMPVIMMSGHATIDTAVEATRIGALNFLE
                     KPIALQKLLKAVQQALTRGNEPLRAQPAYATRPVAAPQAAPATIAAPQSAPADPPPTA
                     SAQANAPMFPASFELPLREARDAFERAYFEHHLVREGGSMTRVAEKTGLERTHLYRKL
                     KQLGVEPAKLARKG"
     gene            1857..2222
                     /locus_tag="VM406_09990"
     CDS             1857..2222
                     /locus_tag="VM406_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HVL76337.1"
                     /translation="MIRATLLGGGDADAREHAIAAALEPHAQNAVILEGLPAQHNALA
                     GHARLDVVRIAAGCPCCQGNTVLRVHLNRLLRQRPDRLFIGVMAADHVPALRAFLSSP
                     PYDGMLELTQDLLPGSRSR"
     gene            2333..2590
                     /locus_tag="VM406_09995"
     CDS             2333..2590
                     /locus_tag="VM406_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006713246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3567 domain-containing protein"
                     /protein_id="HVL76338.1"
                     /translation="MNLIYNSEQYSVVEFGADERREALRFGGYEIMDKSGKREIFIGG
                     TMARSFREEVENLIATEPTVEEIDDFLGKYDALMRQPVTLH"
     gene            2999..4402
                     /locus_tag="VM406_10000"
     CDS             2999..4402
                     /locus_tag="VM406_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HVL76339.1"
                     /translation="MLHVSAPPSSAQPVILLRLAGCPPQAQEYVDALAAHGLSLVPCG
                     SLESAAGMVERGCRVGLLFVTEEADLAHLDRCEPVVCDTRICWVALVAENLVGKPRMR
                     EFIADHLFNFITLPADFGQVVLALRHAWGMAYIRDTQRLPRTAPAADEMVGATPEMQD
                     LRATLQKIARTDAPVLICGPSGSGKELAALTIHRQSSRAAAPFVAINCNAVPAGRFDA
                     ELFGDENGNPGRLQAAQGGTLFLDAAGDLPPDMQSRILDLLNENRIRRPDGRVIEADV
                     RVMAASCPSLEDYVLQGRFREDLYYRLNVMCVRTPGLADRGPDIEAIARHYFGKFATT
                     HNRSLRGFSKAAMNAMLSHSWPGNVRELINRVQRATVMAEGRFIQPEDLGLERPQDGA
                     QLLSLEEARAAAERAMIRRALAHSRNQISRAASLLGVSRVTLYRLIEKYNLRSETAPI
                     VPGPVLAQRKSDGEVRG"
     gene            4492..5256
                     /locus_tag="VM406_10005"
     CDS             4492..5256
                     /locus_tag="VM406_10005"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HVL76340.1"
                     /translation="MCQLLGMNCVLPTDIVFSFTGFATRGGRTDWHKDGWGIAFFEGA
                     GVRHFVDYEAAVASPVAELIKRWPIKSKNVIAHIRKATQGAVTLENCHPFVRELWGRY
                     WVFAHNGDLKDFHPALDGPFRRVGTTDSELAFCYLLQELRRRFGDASPPLAQLNEALK
                     ELCAGIASHGPFNMMLSDGRALFAHCSTNLYYIVRQYPFTTATLSDEDLSVDFAQYTT
                     PEDRVAVIVTSPLTSNETWTQFQPGELIVFVDGRPV"
     gene            5549..>5838
                     /locus_tag="VM406_10010"
     CDS             5549..>5838
                     /locus_tag="VM406_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVL76341.1"
                     /translation="MRAREFFLARQPILDRKQALVAYELLFRNAAVGPPEITNDLNAT
                     AAVIAHASHLGVEKVIGDALGFVNVDAAALASEILQFLPREQVVLEIMENVQP"
BASE COUNT         1111 a         1914 c         1800 g         1013 t
ORIGIN      
        1 ttccggctgg tcagttgcaa cgaatcggtc gaacgtattc ttcagcatga cttcgatgac
       61 tgcatcggaa agccgctcac gacaataccc ggcctcgaac cgttcgccga gtcggtaatg
      121 ggtgcattct ccgagcaaag cgcgcgcgcc gccggagaag gcggtgtagc gcactggcag
      181 aagcagatcg aggtgccacg ccgcaccggc gggccgtcag aggtggacaa caacatcacg
      241 ctgctcacgc gcggctccta cctccccgtc tcgggcgacg tcggacacat cgtggttttt
      301 gacgacatca ccgaggtcat ttccgcgcag cgctcgatcg cctgggcgga agtggcgcgc
      361 cgactcgcgc atgagatcaa gaatccgctc acgccgatcc agctctcggc cgagcgcctg
      421 cagatgaaac tggaaaccaa gctcgagccg gccgacaagg ccgtactgtc caagggcacc
      481 ggcacgatcg tcagccaggt ctcggcgatg aagcgcatgg tcgacgattt ccgcgactac
      541 gcgaaaacac ccgcggcggt gctcgtgccg cttgacctga acgcgctggt cgaggaaatc
      601 ctgcagctct acatggccga catcgagcgc ggagtgatcc atccgcggct cacgcctgaa
      661 ttgccgctgg tgatgggcga cgcgacacag ctgcgccagg tcatccacaa cctgctgcag
      721 aatgcgctcg acgccgtcgg ggacgcggac aacgatgccc cgttcatcga ggtgcttacc
      781 gaaaccgtcc actaccagca tggcgacggc agccgccgta ccgcggtgcg tctgtgtgtg
      841 accgacaacg gccccggctt ctcgccgaag atcctcgcgc gcgcctgcga gccgtatgtt
      901 acgaacaaac cgcgcggcac cggactgggg ctcgcggtgg taaagaaaat cgtggaagaa
      961 cacggcgggc ggattgacat ccggaaccgc tcaaccggaa gcggcgcaaa ggtggcaata
     1021 ttgctgatga agttgtccgg cggacaggca cccgaagcca cctcggctga cgcgaggcag
     1081 gcggcgcggg cagaagaaca cggtcatggc gcgggtattc cgcccggtac ggccggaata
     1141 gagacttgaa taggtagaga catggcaaat attctggtgg tcgacgatga aatgggcatc
     1201 cgcgaactgc tcacggagat cctcggtgat gaaggccatg cggtcaccgc agcagaaaat
     1261 gcgcaggagg cgcgcgaaca gcgcgcgcgc gaacagccgg atctggtcct gctcgatatc
     1321 tggatgcccg acaccgacgg tgtcacattg ctgaaggaat ggcagcgtga cggcctgctg
     1381 acgatgccgg tgatcatgat gtccggccat gcaaccattg acacggccgt ggaggccact
     1441 cgcatcggcg cactgaattt ccttgaaaag ccgattgcgc tgcagaagct gctcaaggcg
     1501 gtccagcagg cgctcacacg cggcaacgag ccgctgcgcg cgcagcctgc ttacgcgacg
     1561 cgcccggtgg cggctccgca ggccgcaccc gcgacaatcg ccgcccccca gagtgcgccc
     1621 gccgatccac cgccgaccgc atctgcacag gcgaacgcgc cgatgtttcc ggcctcgttc
     1681 gaacttcccc tgcgcgaagc acgcgacgca ttcgagcgcg cctacttcga acatcatctc
     1741 gtgcgcgaag gcggaagcat gacgcgggtg gccgaaaaga ccggtcttga acgcacccac
     1801 ctttaccgca agctcaagca gcttggcgtc gagcccgcca agctggcgcg caaggggtga
     1861 tccgcgcgac gctgctcggc ggcggcgatg ccgacgcccg cgaacacgca atcgcggccg
     1921 ccctcgagcc gcatgcgcag aatgcggtga ttctcgaagg tttgccggcg cagcacaatg
     1981 cgcttgccgg ccacgcccgc ctggacgtgg tgcgcatcgc cgcaggctgt ccttgctgcc
     2041 agggaaatac ggttctgcgc gtgcatctga accgcttgct ccgtcagcgc cccgatcggc
     2101 tgttcatcgg ggtcatggcg gcggaccatg ttccggcgct gcgtgcattc ctttcgagcc
     2161 cgccctatga cgggatgctc gaactgactc aagatttgct gcctggcagc cgttcacggt
     2221 agtacccgga gcgaagattt gcgggacggg ttgaaaatcc tcgggctggc ctcatttttg
     2281 catcaacgga aattgagcaa gccgcatcaa atccgatcac gaggagcgca acatgaacct
     2341 gatctacaac agcgagcagt acagcgtggt ggagttcggc gccgacgaac gccgggaagc
     2401 cttgcgcttt ggcggatatg aaatcatgga caaatccggc aaacgtgaaa tcttcatcgg
     2461 cggcacgatg gcccgatcct tccgcgagga ggtcgagaac ctgatcgcca ccgaaccgac
     2521 tgtggaagaa atcgacgact tcctcggtaa atatgacgca ttgatgcggc agcctgtcac
     2581 attgcactga cgggcatgac ggtctgttcc cccgagggaa cagaccgttt tctgcgggca
     2641 ccggctgaac cgccgcatcg tgccggacat cgtgaccgct gccggacgcc tctgcagccg
     2701 caacgtttcc tagcgcatcc atcccagccc gcccaattat ccccaccgac atttttcgcc
     2761 gccccggagc gccgctcgcg acgccgcccg gaaacgctca ttgagcccgc acaagccgaa
     2821 ctgtctccgc cctccaatat ccccaattta tggcattgtt ggagctacca ataggcggta
     2881 atctcgcttc gccccttggc tccaggcggc ccgccggagc gcaagtgcaa agcgagcgtc
     2941 ccaagccggc cttggccggt aaacgcttgc gcgcatcaat aagaaaaggt atcgcgaaat
     3001 gctgcacgta tcggcccctc cctcatcggc ccagccggtc attcttctgc gcctggccgg
     3061 atgcccccct caggcccagg agtacgtgga tgcactggcc gcgcatggcc tgagcctcgt
     3121 cccttgcggg tccctcgaat ccgccgctgg catggtggag cgtggctgcc gcgtgggcct
     3181 gctgttcgtg acggaggaag ccgatctcgc gcacctggac cgctgcgaac ccgtggtatg
     3241 cgatacgcgc atctgctggg tggcgctggt cgccgaaaat ctggtgggca agcctcgcat
     3301 gcgcgaattc atcgccgacc atctgttcaa tttcatcacc ctgccggccg acttcggtca
     3361 agttgtcctc gcgcttcgcc atgcttgggg catggcctat atccgcgata cgcagcgact
     3421 gccgcgcacg gcgcccgccg ccgacgagat ggtaggggct acgccggaaa tgcaggattt
     3481 gcgtgcgacc ctgcagaaga tcgcccgcac ggatgcgccg gtactgatct gcggtccttc
     3541 cggcagcggc aaggaactcg ccgcgttgac gattcaccgc cagtccagcc gagccgcggc
     3601 gcccttcgtg gccatcaact gcaacgccgt gccggcaggc cgattcgatg ccgagctgtt
     3661 cggcgatgag aacgggaatc cgggacggtt gcaggccgcg caaggcggca cgctgttcct
     3721 cgacgcggcg ggcgatttgc cgccggacat gcaatcgcgc attcttgacc tgctgaacga
     3781 aaacaggatc cgccggcccg atggccgcgt cattgaagca gacgtgcgcg tgatggcagc
     3841 atcctgtccc agcctggaag attacgtgct gcaagggcgg ttccgcgagg atctctatta
     3901 ccgtctcaac gtgatgtgcg tgaggacacc cggcctggct gaccgcggtc ccgacatcga
     3961 agccatcgca cgccactatt tcggcaaatt cgccaccacg cacaaccgca gtctgcgggg
     4021 tttttccaag gcggcgatga atgcgatgct gtcacacagc tggccgggaa atgtgcgcga
     4081 gctgatcaac cgcgtgcagc gggccacggt catggcggag ggacgcttca tccagcccga
     4141 agatctcggc ttggaacgcc cgcaggacgg tgcccagctg ctctcgctgg aggaggcacg
     4201 cgctgccgct gaacgcgcga tgatccggcg tgcgcttgcg cattcgcgca atcagatctc
     4261 gcgcgcggca tcgctgctgg gcgtctcgcg tgtgaccttg taccgactga tcgagaagta
     4321 caacctgcgt tcggaaacgg ccccgatcgt gcccggcccg gtgctggcgc agcgcaagag
     4381 cgatggtgaa gtacgtggct gagtctggcg ccgcatgatt cgacgcacgt cgccacgcca
     4441 tgcgcggcga cgtgctatgt tttcattccg gcccgtttca tgtcgcttcg tatgtgccag
     4501 cttctcggaa tgaattgcgt cctgcccacg gacatcgtgt tcagcttcac cggattcgcg
     4561 acccgcggcg gccgcaccga ctggcacaag gacggctggg gaattgcctt cttcgaaggg
     4621 gccggggtgc gccacttcgt cgactacgag gcggcggttg cctcgccggt cgccgagctg
     4681 atcaagcgct ggccgatcaa gtccaagaac gtgatcgccc acatccgcaa ggctacgcag
     4741 ggcgcggtga cgctggaaaa ctgccatccg ttcgtgcgcg agctgtgggg gcggtactgg
     4801 gtgtttgccc acaatggaga cctgaaggac ttccatcccg ccctcgacgg cccgttcagg
     4861 cgcgtcggca cgaccgacag cgagctggcg ttctgctacc tgctgcagga gttgcgccgc
     4921 cgcttcggcg acgcctcgcc cccgcttgcg caactcaatg aagcgctgaa ggaattgtgc
     4981 gccggcatcg ccagccacgg gcctttcaac atgatgcttt ccgacggacg cgcactgttc
     5041 gcgcactgtt caacaaacct gtactacatc gttcgccagt accctttcac caccgccacc
     5101 ctgtccgacg aggacctgag cgtcgatttc gcccagtaca cgacgccgga ggatagggtg
     5161 gcggtcatcg tcacctctcc gctgacgagc aacgaaacct ggacccagtt ccagcccggc
     5221 gaattgatcg tcttcgtcga cggccgaccg gtctgaacga cggcttcctg cacgacccga
     5281 cgaaaattgt gcacaagaaa ctaaaagtcg actaaagtgg aaaggacggt gcggccctcg
     5341 cgttgcgttc atccagagcg tgcacaggcc gtatgcgttg ccgttcctcc gtgcttccgc
     5401 agtgccggaa gaagtgcggc ggcggggacc catacgacct gtgcggatgc gatccagccc
     5461 ggtccgcttg ctatcgcttg ctatccatag agggagcagc acaaccttga acaccgcgca
     5521 gagccccggc cgggccgcgc cgccctcgat gcgcgcgcgc gagtttttcc ttgcccgcca
     5581 accgattctc gaccgcaagc aggcgctggt cgcgtatgag ctcctgtttc gcaacgccgc
     5641 cgtcggcccg ccggaaatca ccaacgatct caatgcgact gcggccgtca tcgctcacgc
     5701 ctcccatctc ggcgtggaaa aggtgatcgg cgacgccctc ggcttcgtca acgtcgacgc
     5761 ggccgcgctg gcaagcgaga tcctgcaatt cctgccccgc gagcaggtcg tgctcgaaat
     5821 catggagaac gtacagcc
//
LOCUS       DATLGF010000327         6330 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_73003,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000327 DATLGF010000000
VERSION     DATLGF010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73003"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VM406_10015"
     CDS             complement(<1..523)
                     /locus_tag="VM406_10015"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HVL76342.1"
                     /translation="MKPACKPATAVSTRTPPRAIGIAAGLAVIAIFAGFTLASRAGYA
                     GALGPADIIALRFGVGGMILLPLFARHRLAGLSAAQAGALAASGGIGFALVAYNGLAL
                     APASHGAVLMHGTLPLFTFGVISLIAPEHVGIRTWAGLSLIGAGILLMAGDSIAGASP
                     RQLAGDGLLLAGSL"
     gene            613..3519
                     /locus_tag="VM406_10020"
     CDS             613..3519
                     /locus_tag="VM406_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVL76343.1"
                     /translation="MPAPRPPDRIADEPCECGAVHSSGTRRKPKIFMAARGTTVDPQQ
                     LLAIVRDLARESRGAQVSYLTPHTSLERDLGLDSLARTELFLRASKAFDAEIPPELVP
                     AIDTVDDLLRALERGVAQGAAPLREMPVAPQALDLPHAAQTLVDVLEWHAARQPERTH
                     VLLHDERHRETPITHRNLLERAQRIARGLLARGLPPRRAVALMLPTGRDYLDTFFGVM
                     LAGGIPVPIYPPARLSQLEEHLRRHARILDNAQAVLIVTVEQARGVAVRLQAAVPELA
                     DIVTAAELRAAAASDTGGGGDADALPALGPDDTAFLQYTSGSTGDPKGVVLTHANLLA
                     NVRALGKAAAVRPDDRFVSWLPLYHDMGLIGAWFGSLYHGIPLVLASPQAFLARPTLW
                     LELISRHRGSISAAPNFAYALCVRHISDAALARLDLSSWRLALNGAEPVSVDTLEGFA
                     GRFARCGLHRSAITPVYGLAECSVGLAFPPPQRGPRIDAVRRAPFERDGRADPAVPGD
                     PDALRLPACGRALAGHEIRVVDEAGIELPERRIGRLEFRGPSATSGYFRNPAATAALL
                     RGGWLDSGDYAYLAEGEVFITGRVKDLIKRAGRALYPYDLEEAVGNLPGVRKGCVAAF
                     SSPDRATGSERLVLLVETREKNEAARSALQQRVQRTVIEVIGAPAEEVVLVPPHAVLK
                     TSSGKIRRQACRERYERGELEQAARPAWISALAYSAGKMQARMRVGVRRAGAALFGCY
                     AWLVLAALALPGIILLAASRSTETARAAARRVARALFALTGVALRVHHLERLPPHPHV
                     LLVNHASYLDAIALTAALPAPPGYAYAAKAEFAGQFFMRTLLRRLGAVFVERQDTAHG
                     AREVEAMAALLARGESLLVFPEGTLSHSPGIHTFHVGALLAAGRAGVPVVVAGLRGTR
                     TALRSGSWWPHRDTLELEIGPILHPEGNDWKAATLLAARARAAMAPLAGEYLVP"
     gene            3591..4550
                     /locus_tag="VM406_10025"
     CDS             3591..4550
                     /locus_tag="VM406_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HVL76344.1"
                     /translation="MAHFDVFNGDADGICALHQLRLEDPRASDLITGVKRDIALLGRV
                     PAQAGDAVTVLDISIDVNRAPLLALLQRGVSILYFDHHGSGQVTPHPALDAHIEPLPG
                     TCTSTLVDRWLGGRRRPWAVVGAFGDNMGATARALAATLWLDEGRTERLRVLGEALNY
                     NGYGDSIEDLMIHPAELYRLLHAHTDPFGFIENEPICAKLVAGRQADMAAAHGTAALL
                     ERSQGRIVVLPDAAWSRRVRGSYGNELAVAQPQRAHAVLSPDRAGGYVVSVRAPIDRP
                     VGADALCQRFPGGGGRPAAAGINHLPADRLDAFARAFEQAFCA"
     gene            complement(4617..5456)
                     /locus_tag="VM406_10030"
     CDS             complement(4617..5456)
                     /locus_tag="VM406_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVL76345.1"
                     /translation="MGYKTILVHVDEARNIDTRIALAAELALREDAHLVGAASTGVSR
                     YVYQSMVASIDSGVIDGYMDALQERAERALRHFEEVVRRIGVASWEARPAMDEPAGGL
                     SLQARYCDLCVLGQFDPDESAPLVQPNLREYVAIHGGAPVLVVPNRWNRTSPGERCLV
                     AWNASVDAKHALQAAIPMLRAAGSVDIVVFQNGELDELAGEQPGLDVAAFLARHGIPV
                     SLQERRVDGEVGHALLALATEVRADMLVMGCYGHSRFREILLGGATRTVLESMHLPVL
                     MAH"
     gene            complement(5486..>6330)
                     /locus_tag="VM406_10035"
     CDS             complement(5486..>6330)
                     /locus_tag="VM406_10035"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HVL76346.1"
                     /translation="ITESTARWRGAGKAFYFGAPPQAADCLARAGVDLVSLANNHALD
                     FDYAGLADTFRILQARGIAWAGAGEDERAATSVATLARGGLRFGMAAFCDHQQDFAAG
                     PATPGIAWLDLADEAGTLHRWRAALDDLHEAGVNWPILSLHWGSNLAIRPPQRFRRLA
                     HAAIDMGWRILFGHSAHVYQGVELYRGCPILYAAGDLVDDYYVDPELRNDLQFLFELE
                     LDAVRLRAIRMWPLRIDDCRSEPADPSMFAEMAETMIGRCAELGTPVERIDGSLQVAV
                     PEQA"
BASE COUNT          953 a         2224 c         2186 g          967 t
ORIGIN      
        1 acagcgatcc ggccagcagc aggccgtcgc ccgccagttg gcgcgggctg gcgccggcga
       61 tggagtcgcc ggccatcagc agaatgccgg cgccgatcag cgacagtccg gcccaggtgc
      121 gtatgccgac atgttccgga gcaatgaggg aaatgacgcc gaacgtaaac agcggcagcg
      181 ttccgtgcat gagaacggcg ccatggctgg ccggcgccag cgccagaccg ttgtaggcga
      241 ccagcgcgaa accgatgccg cccgacgcgg ccagcgcacc ggcctgcgca gcggagaggc
      301 cagcgaggcg atggcgggcg aacaggggca gcaggatcat tccgccgacg ccgaaacgca
      361 aggcgatgat gtccgccggg ccgagtgcgc cggcatagcc ggcacgcgaa gcgagcgtga
      421 agccggcgaa gattgcgatc accgcaagcc cggcggcgat gcctatcgcg cgcggcggcg
      481 tacgggttga gaccgccgtt gcgggtttgc atgctggttt catcgcgttc cctcggtaag
      541 tgccagcagt ctacggagct gctgcctgcg cgctcaaacg agatttgcgc cggcgtgcca
      601 tgcatccacc gcatgccagc gccccgcccg ccagacagga ttgccgatga accgtgcgag
      661 tgcggcgcgg tccacagcag tggaacccgg cgcaaaccca agatcttcat ggccgcacgc
      721 ggcaccactg tcgatccgca acagctcctg gccatcgtgc gcgacctcgc aagagagtcg
      781 cgcggggcgc aggtctccta tctcacgccg catacatcgc tggagcgcga tctgggcctc
      841 gattcgctcg cgcgcaccga actgttcctg cgtgcaagca aggccttcga cgccgaaatt
      901 cctccggaac tggtgccggc catcgacacc gtcgacgact tgctgcgcgc gctcgagcgc
      961 ggggtcgccc agggcgctgc gccgttgcgc gagatgcctg tggcgccgca ggcgctcgac
     1021 ctgccgcatg ccgcacaaac cctggtcgac gtgctggagt ggcatgccgc gcggcagccg
     1081 gaacgcaccc atgtccttct gcacgacgag cgccaccgtg aaacaccgat cacgcaccgc
     1141 aacctgctcg agcgtgcgca gagaatcgcg cgcgggctgc tggcgcgcgg gctgccgccg
     1201 cgtcgggcgg ttgcgctgat gctgcccacc gggcgcgact atctcgacac cttcttcggc
     1261 gtgatgctgg ccggtggcat tccggtaccg atctatccgc cggcccggct gtcccagctt
     1321 gaggaacacc tgcgccgcca cgcgcgcatc ctcgacaacg cgcaggcggt cctgatcgtg
     1381 acggtcgaac aggcgcgcgg tgtcgcggtg cgcctgcagg cggcggttcc ggagctggcg
     1441 gacatcgtga ccgctgcgga actgcgggcg gcggccgcgt ccgacaccgg cggcggcggc
     1501 gacgcggatg cactgccggc gctcgggccg gacgacaccg cctttctgca gtacacatcc
     1561 ggcagcaccg gcgaccccaa gggcgtggtg ctgacgcatg cgaacctgct cgccaatgtg
     1621 cgtgcgctcg gcaaggcggc cgccgtgcgg cccgacgacc gcttcgtgtc ctggctgccg
     1681 ctgtaccacg acatgggcct gatcggcgca tggttcggct cgctgtacca cggcattcca
     1741 ctggtgctcg cttccccgca ggccttcctg gcgcgtccca cgctgtggct cgaactgatc
     1801 tcgcgccatc gcggcagcat ctcggcggcg cccaactttg cctatgcgct gtgcgtgcgt
     1861 cacatcagcg acgccgcgct tgcgcggctg gacctgtcga gctggcgcct tgcgttgaac
     1921 ggcgccgagc cggtcagcgt cgatacgctg gagggcttcg ccggccgctt tgcgcgctgc
     1981 ggcctgcacc gcagcgcgat cactcccgtc tatggactgg ccgagtgctc ggtcgggctg
     2041 gccttcccgc cgccgcagcg cggaccgcgc atcgacgccg tgcggcgcgc gccgttcgag
     2101 cgggacggcc gggcggaccc ggcggtcccg ggtgatccgg acgcgctgcg gctgcccgcc
     2161 tgcggccgcg cgctggccgg gcatgagatc cgcgtcgtcg acgaggccgg catcgagctg
     2221 cccgagcgcc gcatcggtcg cctggaattc cgcggcccgt ctgcgacgtc cggctatttc
     2281 cgcaatccgg cggccaccgc tgcgctgctg cgcggcggct ggctggactc gggcgactat
     2341 gcctatcttg ccgaaggcga agtgttcatc accggccgcg tgaaggactt gatcaagcgc
     2401 gccgggcgcg cgttgtatcc gtatgacctg gaggaagcgg tcggcaatct gccgggtgtg
     2461 cgcaagggct gtgtcgccgc gttctccagc ccggaccgcg ccaccggaag cgaacggctc
     2521 gtgctgctcg tcgagacgcg ggagaagaac gaagcggcgc gctcggcatt gcagcagcgc
     2581 gtgcagcgca ccgtgatcga ggtgatcggg gcgccggccg aagaggtggt gctggttccg
     2641 ccgcacgcgg tgctgaaaac gtccagtggc aagatccgcc ggcaggcttg ccgggaacgc
     2701 tatgaacgcg gcgagctgga gcaggctgcg cgccccgcct ggatcagcgc gcttgcgtac
     2761 agcgccggaa agatgcaggc ccggatgcgc gtcggcgtgc gccgtgccgg cgcggccttg
     2821 ttcggctgct atgcgtggct ggtgctggcg gcgctcgccc tgcccggcat catcctgctg
     2881 gcggcctcgc gcagcaccga gaccgcacgc gcggcggcgc gcagggtcgc acgggcgctg
     2941 ttcgcgctca cgggggtagc gctgcgtgtg catcatctgg agcgccttcc gccccatccg
     3001 catgtgctgc tggtcaatca cgcaagctac ctggacgcga tcgccctgac cgccgccctg
     3061 cccgccccgc cgggctatgc ctatgccgcc aaggcggagt tcgccggcca gttcttcatg
     3121 cgcacgctgc tgcgacgact tggcgcggtg ttcgtcgagc ggcaggatac cgcccacggc
     3181 gcgcgcgagg tcgaagcgat ggcggcgctg ctggcgcgcg gggagagtct gctcgtattc
     3241 ccggaaggca cgctgtcgca ctcgcccggt atccatacct tccatgtggg cgcgctgctg
     3301 gccgccgggc gcgccggcgt gccggtcgtg gtggccgggc tgcgtggcac ccgcactgcg
     3361 ctgcgcagcg gcagctggtg gccgcatcgc gatacgctcg aactcgaaat cggtccgatc
     3421 ctgcatcctg aaggaaacga ctggaaggct gcgaccttgc tggccgcgcg tgcacgcgcg
     3481 gcaatggcgc cgctggccgg agagtacctg gtgccatgac gccatgaccg ggatcaattg
     3541 ccgcgtcccc gggcgttttc gcggcggccg cgcgacgcga tgatcggcat atggcgcatt
     3601 tcgacgtatt caacggcgac gcggacggaa tctgtgcgct gcaccagctg cggctggagg
     3661 atccgcgtgc gtccgacctg atcaccggag tcaagcgcga catcgcgctg cttggccgtg
     3721 tgccggcgca ggccggcgac gccgtcaccg tgctcgacat atccatcgac gtgaaccgcg
     3781 cgccactgct tgcgctgctg cagcgcggcg tcagcattct ctatttcgat caccatggct
     3841 ccggccaggt gacgccgcat ccggctctcg acgcgcacat cgagccgctg cccggcacct
     3901 gcacgtccac gctggtagac cgctggctcg gcgggcgccg gcggccctgg gcggtggtcg
     3961 gcgcgttcgg cgacaacatg ggtgcgactg cacgtgcgct cgcggccacg ctgtggctcg
     4021 acgagggccg gaccgagcgc ttgcgcgtgc ttggcgaggc gctcaactac aacggttacg
     4081 gcgacagcat cgaagacctg atgatccacc cggccgagct gtaccgcctg ctgcacgccc
     4141 acaccgaccc gttcggcttc atcgagaacg aaccgatctg cgcgaaactc gtcgcgggcc
     4201 gtcaggcgga catggcggcc gcgcatggca ccgctgccct tttggagcgc tcgcaggggc
     4261 gcatcgttgt gctgcccgac gcggcctgga gccggcgcgt gcgtggcagc tacgggaacg
     4321 aactggcggt tgcgcagcct cagcgcgcgc acgcggtgct ctcgcccgac cgcgcgggcg
     4381 gctacgtggt cagcgtgcgc gctccgatcg atcgcccggt cggggccgat gcgctttgcc
     4441 agcgctttcc cggtggcggc gggcggccgg cggcggccgg catcaatcac ctgccggccg
     4501 accggctcga cgcgtttgca cgcgcgttcg agcaggcgtt ctgcgcatga tgcggctttg
     4561 aagcgccaac acgaaacgcc gatggcaagg aggcgcaacg ccgcagttag ggccgcttaa
     4621 tgcgccatca acaccggcag atgcatagac tccagcaccg tgcgcgtggc gccgccgagg
     4681 aggatctccc gaaagcggga atgaccgtag cagcccatga ccagcatgtc cgcccgcact
     4741 tcggtggcaa gggccagcag cgcgtggccg acctcgccgt ccacgcgccg ctcctgcagg
     4801 ctgaccggaa tgccatgccg cgccaggaag gccgcgacgt caaggccggg ctgctctccc
     4861 gccagctcgt ccagctcacc gttctggaac acgacaatgt ccaccgatcc ggcggcgcgc
     4921 agcatcggta tcgcggcttg cagcgcgtgc ttggcatcga cgctcgcgtt ccaggccacc
     4981 aggcagcgct cgcccggcga cgtcctgttc cagcgattgg ggacgaccag taccggcgcc
     5041 ccgccatgga tcgcgacgta ttcgcgcaga ttgggctgga ccagcggcgc gctttcatcc
     5101 gggtcgaact gaccgagcac gcacaggtcg cagtagcgcg cctgcaggct caggccgccg
     5161 gccggctcgt ccatcgccgg ccgcgcttcc caggacgcca cgccgatgcg gcgcacgacc
     5221 tcttcgaaat ggcgcagcgc gcgttccgcc cgttcctgca gcgcatccat gtagccgtcg
     5281 atcacaccgg aatcgatgct cgcaaccata gactgataca catagcggga gacgccggtc
     5341 gaagcggcgc cgaccaggtg cgcgtcctcg cgcagcgcca gttcggccgc cagcgcgatg
     5401 cgggtatcga tgttgcgtgc ttcgtccacg tgaaccagta tcgtcttgta tcccatgctt
     5461 gactcctcat tgcctgcgcc ggcgctcagg cctgttccgg cactgccacc tgcaagctgc
     5521 catcgatccg ctcgaccggc gttcccagct ccgcgcagcg gccaatcatc gtctcggcca
     5581 tctcggcgaa catggaagga tctgccggtt cgctgcggca gtcgtcgatg cgcagcggcc
     5641 acatccggat tgcgcgaagc cggacggcat ccagctccag ctcgaacagg aactgaaggt
     5701 cattgcgcag ctccgggtcc acgtaatagt cgtcgaccag gtcaccggcc gcgtagagaa
     5761 tcggacagcc gcgatacagc tcgacgccct ggtagacatg ggcgctgtgg ccgaacagga
     5821 ttctccagcc catgtcgatc gccgcatgcg cgaggcggcg aaagcgctgc ggcggccgta
     5881 ttgcgagatt cgagccccag tgcagcgaga ggatcggcca gttgacgccg gcttcatgca
     5941 ggtcgtccag cgctgcgcgc cagcgatgca gcgtgcccgc ctcgtccgcg agatcgagcc
     6001 aggcgatccc cggcgtagcc ggccccgccg caaaatcctg ctggtgatcg cagaacgcgg
     6061 ccatgccgaa gcgcagcccg ccgcgcgcga gcgtcgctac gctggtcgcg gcccgctcgt
     6121 cctcgccggc gccggcccag gcaatgccgc gcgcctgaag aatccggaag gtgtcggcaa
     6181 ggccggcgta gtcgaaatcc agcgcgtgat tgttggcgag gctcacgagg tcaacgccgg
     6241 cgcgcgccag gcagtctgcc gcctgcggcg gcgcgccgaa gtagaacgcc ttcccggcgc
     6301 cgcgccagcg tgcagtcgat tcggtgattg
//
LOCUS       DATLGF010000328         3966 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_92658,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000328 DATLGF010000000
VERSION     DATLGF010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92658"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /locus_tag="VM406_10040"
     CDS             <1..1308
                     /locus_tag="VM406_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVL76347.1"
                     /translation="PQDNHGVAVGLDHDRADRFFGKFAMGALNLSVAHSKRTKGVPTA
                     SFSQVFNDPRSHTTDTHTMVDIGYRHGLSAGTELASRLFWGSHDYDGDYIYDYPPVTI
                     NRDTSRGRWWGGELRLTSAHDGHKLVLGTEFQNDYRRDQAIFDVDPFLSYLDSRRDGH
                     RWGVFAQDEWRLRENLLLNAGLRYDKHSSNASAFNPRLGLVYQLSAATTLKAVYATAF
                     RAPNAYELYYDLRMAGGHKANPELGPERIRSGELIVEHYLSPSARISATLFRNKVRDL
                     IALRVDPLDGLVMFENIDRAQAQGIGIELEKAWSTGMRLRTNLSLQQTRDAISGEALP
                     NSPRRLAAFNLSAPVFGGTWRSGIEARYVGPRNTLSGGQVDGYWLANLTLTSVRLGPG
                     LEAGLSVYNLFNRRHADPGTAEHAQDAIAQDGRTVWLRLGYAF"
     gene            1298..1852
                     /locus_tag="VM406_10045"
     CDS             1298..1852
                     /locus_tag="VM406_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003276753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HVL76348.1"
                     /translation="MRSERGATPSSGPRRLAGALAAAWLFAVPAHAGAQPVAEPDLKA
                     AFIYNFTLFTEWPAEAGFEGGALNVCVNPASGLREPLQALTDKPVRGRRMAVRVLTAV
                     DGLRGCDALVLDDADRARGGALIAAARALPVLTIADGPEVAGSIIALAREGDRLVFDV
                     DTAAARRSNLKLSSRLLRLARTVR"
     gene            1855..3738
                     /locus_tag="VM406_10050"
     CDS             1855..3738
                     /locus_tag="VM406_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVL76349.1"
                     /translation="MHTRTRLPLGRWMYRANLVSAGAALLVASVLLILFQFLALRDDL
                     IEDARVQARIVGDNSAAPLLFDDARAAYETLAALNSAPSIVSAAVYATDGTLVAGYRR
                     DGADVVPALRNVLTTDHALTPSRLELAHPIFVDGREVGVLVLHLTLEQIYGRIAGFAS
                     LTLAIAFGSFIVAWLLVARMRGVVRKAESHLEYLAHIDPVSGLPNRHAFNERLAQTLA
                     QVDRHGGGASLLLIDLDNFKIVNDTLGHHCGDRLLHQVAQRLLACLSGGEEICRIGGD
                     EFAIIQQAASYSAAQAAAQKLMTGLAEPFNVDGNEIYVTASVGISRYPDDAGDVETLM
                     RNADTAMYQAKGKGKNAYESFHPELDRRIQKRRALEMHLHKAVERGELQLHYQPQVSL
                     RTGRLVGLEALLRWNNGELGQVPPQEFIPVAEESGLIVPIGRWVLRQACAQIAAWRDG
                     GLGNVQVAVNLSARQTRDPHLMQDILDALRDHKVAPRQLELEITESVLMDNVLANVAL
                     LHRLQTEGIRLSIDDFGTGYSSMAYLKRFPVEQVKIDRTFVRDVPGDRDDEAITSAII
                     AMAHNLGLSVVAEGVENEQQLLFLRNAGCDTMQGYYFAKPMPPEQVAEFLRMRLAGQE
                     VVV"
BASE COUNT          666 a         1306 c         1337 g          657 t
ORIGIN      
        1 ccgcaggaca accacggcgt cgcggtcggg ctcgaccacg accgcgccga ccgcttcttc
       61 ggcaagttcg cgatgggtgc actgaacctg tcggtcgcgc acagcaagcg cacgaagggg
      121 gttcccacgg cttccttcag ccaggtgttc aacgacccgc gttctcatac caccgatacc
      181 cacaccatgg tcgacatcgg gtaccggcac ggcctgtctg ccggcaccga gctggcgtcg
      241 cgcctgttct ggggcagcca tgattacgat ggcgactaca tctatgacta cccgcccgtc
      301 acgatcaacc gcgacacctc gcgcggccgc tggtggggcg gcgaactgcg gctgacgagc
      361 gcgcatgacg gacacaagct cgtgctgggc acggagttcc agaacgacta ccggcgcgac
      421 caggcgatct tcgatgtgga ccccttcctg tcgtatctcg attcgcgccg cgacgggcac
      481 cgctggggcg tgttcgcgca ggatgaatgg cgtctgcgcg agaacctact gctcaacgcc
      541 ggccttcgct acgacaagca ttccagcaac gccagcgcat tcaatccccg cctgggcctg
      601 gtctatcagc tcagcgcggc aacgacgctg aaggccgtct acgccaccgc gtttcgcgcg
      661 ccgaacgcct acgagctcta ttacgacctg cgcatggccg gcgggcacaa ggcgaatcca
      721 gagctggggc ctgagcgcat tcgctcgggc gagctgatcg tcgagcacta cctgtcgcca
      781 agcgcgcgca tctccgccac gctgttccgc aacaaggtgc gcgatctgat cgcattgcgg
      841 gtggatcccc tggatggtct ggtgatgttc gagaacatcg accgtgcaca ggcgcagggc
      901 atagggatag agctggagaa ggcatggagc acagggatgc gcctgcgcac caatctcagc
      961 ctgcagcaaa cccgcgacgc catcagcggc gaggcgctgc cgaattcgcc gcggcgcctg
     1021 gcggccttca acttgtcggc cccggtcttc ggcgggacgt ggcgcagcgg catcgaagcg
     1081 cgctatgtcg gcccaagaaa taccttgagc ggcgggcaag tcgacggcta ctggcttgcc
     1141 aatctgacgc tgacctcggt tcggctgggg ccggggctgg aggccggact cagcgtctac
     1201 aacctgttca accgccgcca tgccgatccc ggcacggcag aacacgcgca ggacgccatc
     1261 gcccaggacg ggcgcacggt atggctgcgc ctgggttatg cgttctgagc gtggtgcgac
     1321 accgtcgtct ggtccgcggc gcctggcagg cgcgcttgcg gcggcctggc tgttcgccgt
     1381 tccggcgcat gcgggggcgc agccggttgc cgaaccggac ctgaaggccg cattcatcta
     1441 caacttcacg ctgtttaccg aatggccggc cgaggctggg ttcgagggcg gggcgctgaa
     1501 tgtctgcgtg aatccggcca gcggtctgcg cgagcccttg caagcgctca ccgacaagcc
     1561 ggtgcgcgga cgcaggatgg cggtacgcgt gctgacagct gtcgatggcc tgcgcggatg
     1621 cgatgccctg gtgcttgacg acgccgaccg cgcacgcggg ggcgcactga ttgccgccgc
     1681 acgcgcgctg cccgtcctca ccatcgccga cggcccggaa gtcgccggca gcatcatcgc
     1741 cctcgcgcgc gagggcgacc ggctggtgtt cgatgtcgac acggccgccg cgcgccgttc
     1801 caacctgaag ctcagctcgc gcctgttgcg gctggcgcgt accgtacgat gaccatgcac
     1861 acccgcacac gcttgccatt ggggcgctgg atgtaccggg ccaacctggt ctcggccggc
     1921 gccgcgctgc tggttgccag cgtgctattg atcctgttcc agtttctggc gctgcgcgac
     1981 gacctgatcg aggatgcgcg cgtgcaggcc cgcatcgtcg gcgacaacag cgccgcgccg
     2041 ctgctctttg acgatgcgcg cgccgcctat gaaacgctgg ccgcgctcaa tagcgcgcca
     2101 agcatcgtct cggcagccgt gtatgcgact gacggcacgc tggtggccgg ctaccggcgc
     2161 gacggcgccg acgtggttcc ggcgctgcgc aacgtgctca caaccgatca tgcgctcacg
     2221 ccgagccgtc tcgaactggc gcatcccatc tttgtcgacg ggcgcgaggt cggagtgctc
     2281 gtgctgcacc tcacgctcga acagatctac ggtcgtatcg ccggctttgc cagcctgaca
     2341 ctggcgatcg cgttcggctc gttcatcgtg gcctggctgc tggttgcccg catgcgtggc
     2401 gtggtgcgca aggccgaatc gcatctcgaa tacctggcgc atatcgaccc tgtcagcggg
     2461 ctgccgaacc gccacgcgtt caatgaaagg ctggcgcaga cgctcgcaca ggtggatcgc
     2521 cacggcggcg gcgcgtcgct gctgctgatc gacctggaca acttcaagat cgtcaatgac
     2581 accctcggcc accactgcgg agaccggctg cttcaccagg tcgcgcagcg cctgctcgca
     2641 tgcctgtccg gcggcgagga gatatgccgc ataggcggcg acgaattcgc gatcatccag
     2701 caggcggcca gttattcggc ggcgcaggcg gcggcgcaga agctcatgac cgggctggcc
     2761 gagccgttca atgtcgacgg caacgagatc tacgtgacgg cgagcgtcgg catcagccgc
     2821 tatccggatg acgccggaga cgtcgaaacc ctgatgcgca acgccgatac ggcgatgtac
     2881 caggccaagg gcaagggcaa gaatgcctac gagagcttcc atccggagct cgatcgccgg
     2941 atccagaagc gccgggcgct ggagatgcac ctgcacaagg ccgtcgagcg cggggagctg
     3001 cagttgcatt accagcccca ggtcagtctg cgcaccggcc ggctggtcgg cctcgaggcg
     3061 ctgctgcgct ggaacaatgg cgagctcggc caggttccgc cgcaggaatt catcccggtc
     3121 gccgaggaga gcgggctgat agtcccgatc ggccgctggg tgctgcgcca ggcctgcgcc
     3181 cagatcgcgg cctggcggga cgggggcctg ggcaacgtgc aggtggccgt gaacctgtcg
     3241 gcacgccaga cgcgcgaccc gcatctgatg caggacatcc tcgacgcgct gcgcgatcac
     3301 aaggtcgcac cgcgccaact ggagctcgag atcaccgagt cggtactgat ggacaacgtg
     3361 ctggccaacg tcgcgctgct gcaccggctg cagaccgaag gtatccgcct gtcgatcgac
     3421 gatttcggca ccggctattc atcgatggcc tacctgaagc gctttccggt ggagcaggtg
     3481 aagatcgacc gcaccttcgt acgcgacgtt ccgggcgacc gcgacgatga agcgatcacc
     3541 agcgccatca tcgcgatggc gcacaacctc ggcctgtcgg tggtggccga aggtgtggag
     3601 aacgagcagc agctgctgtt cctgcgcaat gcgggctgcg acacgatgca ggggtactac
     3661 tttgccaaac cgatgccgcc ggagcaggtg gcggagttcc tgaggatgcg gctggcgggg
     3721 caggaggtgg tggtgtaggg gcgggcgtcg gcgtggcagg ggcaggagtc aggagttggg
     3781 cgcttcgctt atcaggcgga tgaggtccct gacgcgggcc gccctcactg cgttccggac
     3841 gacagtagtt tggcgatgat ccccaacatt gtcgttccga gcgaagcgag gaatctcatc
     3901 cgcttggtac gcggcgtcga atgctgggcc gatgaggtcc ctgacgcggg ccgccctcac
     3961 tgcgtt
//
LOCUS       DATLGF010000329         6955 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_102671, whole genome shotgun sequence.
ACCESSION   DATLGF010000329 DATLGF010000000
VERSION     DATLGF010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6955
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102671"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VM406_10055"
     CDS             complement(<1..724)
                     /locus_tag="VM406_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HVL76350.1"
                     /translation="MKPLRHLLIALWLCGLSVAAHAAIDPQLLKPLAGDDPDARIEAV
                     NEIAALGSDDAYRVLSALRNYGLYATGDGSVLVVDGSSAWNPATGETGPVPADADSIT
                     VNNRLRNVLQGALSGFRLFSPDRNVRLAAAQEMQRNADAGQLPLIEQALEKETDARIR
                     DVLEQAAAMARLESDDPALRRSAIEALAGSSNASLVPTLRRLLEKDASGEYIEPDAGV
                     RDAAHATVRQISARLKRNEFISN"
     gene            complement(888..2141)
                     /gene="urtA"
                     /locus_tag="VM406_10060"
     CDS             complement(888..2141)
                     /gene="urtA"
                     /locus_tag="VM406_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HVL76351.1"
                     /translation="MSRRTVLKATAAAALTLMTSAMMPSAMASDTIKVGILHSLSGTM
                     AISETTLKDVALMAIEEINAKGGVLGKKLEPVVVDPASNWPLFAERARQLISQDKVAV
                     VFGCWTSVSRKSVLPVFKELNSLLFYPVQYEGEELERNVFYTGAAPNQQAIPAVEYLM
                     SKEGGGAKRFVLLGTDYVYPRTTNKILRAFLKSKGVKDSDIDEVYTPFGHSDYQTIVA
                     NIKKFSAGGKTAVISTINGDSNVPFYKELGNAGIKATDVPVVAFSVGEEELRGVDTKP
                     LVGHLAAWNYFQSVKSPENDEFVKKWRAYAVAKKLPNAATAVTNDPMEATYVGIHMWK
                     QAVEKAGSTDTDKVIAAMGGQTFKSPSGYTLTMDKTNHHLHKPVMIGEIRPDGQFDIV
                     WKTKGPIRAQPWSPFIPGNEAKQKL"
     gene            2515..3111
                     /locus_tag="VM406_10065"
     CDS             2515..3111
                     /locus_tag="VM406_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HVL76352.1"
                     /translation="MKKNTCARMAWPRASLALAALLATASAHAHPGHGEGVVSGLLHP
                     LLGIDHLLAILATGIWASQASLAGQRLLPASFVGAMGLAALAGLAGMPLQALEPGIAG
                     SVLLVGLLIAFRVQMAPAAGALAVAVFAAFHGYAHGAELPVGASAWQYFAGFLFTTLL
                     LLQAGLKAGPLLARWKAALPTTGAALAAGGAWMLGPLA"
     gene            3208..3843
                     /locus_tag="VM406_10070"
     CDS             3208..3843
                     /locus_tag="VM406_10070"
                     /inference="COORDINATES: protein motif:HMM:NF013306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HVL76353.1"
                     /translation="METMPTDSSALSLLRLATAPLHTRLERGLRIVQPGAGHDEFRAY
                     LVALWGWHCSFEDRLWSYDWPAPLEVTQRRAKLRALAQDLESLGFDEEARDALPRCDY
                     APALDGVAERYGVAYVVEGAQLGTRVLGKSLAPALAPWSPAWLEGYGANAGLSWKTFI
                     ACLERALETPDARAQAAAAAVGAFESLNVWFELCGSRECTCASYPTTPDQT"
     gene            3840..6089
                     /locus_tag="VM406_10075"
     CDS             3840..6089
                     /locus_tag="VM406_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL76354.1"
                     /translation="MSDDPIAAPQPDPALCAQEPIRIPGAIQPHGFLLALHPSEHTIV
                     QLSANAGRFLGRQAGDLIGARLATAVGQDNEADLLDALCRASGRQPYYLGVVRLESGP
                     FDVLGHHHDALLVLEFEPAARAVSADFRLLYPLLGSFLGTLTANDSIMTMSEHAAQAI
                     KGATGFGRVLVYRFDECGHGIVLAEARDDGYHSYLNQRFPASDIPLQARELYLANRIR
                     LIPNADYEPAPLIPADNALTGAPTDLSYAALRSVSPIHLQYMRNMGTLASMSVSLVVK
                     GRLWGLISCHHAHPRHVPFEVRTGCEQLAQILALRIESCEERAQAHHRLELRSLLVAM
                     LAGLTRGPDFIDNITAISPQLLRFASASGAAVVFEQRVVRFGDAPPEADIHKIVEWLA
                     ARPHDEAFHTDALAQLMPEAASMARHASGILALPISSLHRHYLIWFRPEIVHTIDWAG
                     NPHLKDEAGAVPSAVLTPRASFDSWRETVRGRSAAWRPSEIECANEFRAALLGVVLER
                     AEQMAELAEELGRANKELESFSYSVSHDLRAPLRHIAGYSDLLLELEEGQLSERGQRY
                     VRNIKDSARFAGKLVDDLLSFSQMGRASLRPGTVDLNELVASCIEHLADEAKGRELHW
                     EVSPLPIVQADPRFLLLALRNLLSNAVKYTRPKPQARISISAHSSDEETVVQVRDNGV
                     GFNTEYKHKLFGVFQRLHRYEEFEGTGIGLANVQRIVERHGGRVWADSVIGEGAVFTF
                     SLPKQLPTD"
     gene            6097..6552
                     /locus_tag="VM406_10080"
     CDS             6097..6552
                     /locus_tag="VM406_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL76355.1"
                     /translation="MLKPILLVEDNPNDLELTLIALEKSKLANDVVVVRDGAEALSYL
                     NASGDYAHRPAGNPAVILLDLKLPKVDGLEVLRHIRETPPLKSIPVVMLTSSREEQDL
                     LRSYELGVNAYVVKPVEFSEFVQAIADLGIFWAVLNEPPPGSMRYMPRP"
     gene            complement(6568..>6955)
                     /locus_tag="VM406_10085"
     CDS             complement(6568..>6955)
                     /locus_tag="VM406_10085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL76356.1"
                     /translation="RGSILVVEDEAEVRETAVVFLSGLGYTVFQAQDGATALALIESG
                     LVVDLLFTDVIMPGTVRSGELVKHARQKLPRLQVLFTSGYPEGELARDGKLEAHIRLL
                     KKPYGGEALAREIAQMLEPERVGEGE"
BASE COUNT         1168 a         2321 c         2204 g         1262 t
ORIGIN      
        1 ggttggagat gaactcattg cgcttcaggc gcgcgctgat ctgcctgacc gttgcatggg
       61 cggcatcgcg cacgcccgca tccggctcga tgtactcgcc cgaggcatct ttttccagca
      121 ggcggcgcag cgtcggcacc aggctcgcgt tggaactgcc tgcgagcgcc tcgatcgcgc
      181 tgcggcgcag cgccggatcg tccgattcca gccgcgccat tgctgcagcc tgttcgagca
      241 cgtccctgat gcgcgcatcg gtttcctttt ccagcgcctg ctcgatcagc ggcagctggc
      301 cggcatcggc gttgcgctgc atctcctgcg cagcggccag gcgcacgtta cggtccggag
      361 aaaacaggcg gaagccggac agcgcgccct gcagcacgtt gcgcaggcgg ttgttgaccg
      421 tgatgctgtc ggcgtcggcc ggaaccgggc cggtttcgcc ggtggcggga ttccaggcgc
      481 tgctgccgtc gaccaccagc acgctgccgt caccggtcgc atacagaccg tagttgcgca
      541 gcgcgctcag cacacgatag gcatcgtcgc tgccgagcgc ggcaatctcg ttgaccgcct
      601 cgatgcgtgc gtccggatcg tcgccggcca ggggtttcag gagttgcggg tcgatcgcgg
      661 catgggccgc caccgagagg ccgcacagcc agagggcgat caggagatgt cgtagaggtt
      721 tcatcacttt tcgatgcacg cgccgttacg tacaccgcga tcactgcgga cgaaaaaaaa
      781 gccggagcca ggcgtggaga caggctccgg ctgaatgcgc ctcatcccgt aggagaagcg
      841 ggggagcgga acggggcggg agacgtcgcc ccgttcccgt cctcctgtta cagcttttgc
      901 ttggcctcgt tgcccgggat gaacgggctc cacggttggg cgcggatcgg gcccttggtc
      961 ttccagacga tgtcgaactg gccgtcggga cggatctcgc cgatcatcac cggcttgtgc
     1021 aggtggtggt tggtcttgtc catggtgagc gtgtagcccg acggtgactt gaaggtctgg
     1081 ccgcccatgg cagcgatcac cttgtcggta tcggtggagc cggctttctc gaccgcctgc
     1141 ttccacatgt ggatgccgac ataggtcgcc tccatcgggt cgttggtcac cgcggtcgcg
     1201 gcgttcggca acttcttggc aacggcgtag gccctccact tcttcacgaa ctcgtcgttc
     1261 tccgggctct tcaccgactg gaagtagttc catgcagcga ggtggccgac cagcggcttg
     1321 gtgtcgacgc cgcgcagctc ttcctcaccg accgagaatg ccaccaccgg cacgtcggtc
     1381 gccttgatgc cggcgttgcc gagctccttg tagaacggca cgttggaatc gccgttgatg
     1441 gtggagatca ccgcagtctt gccgccggcg gagaatttct tgatgttggc gacgatggtc
     1501 tggtagtcgg aatggccgaa cggggtgtag acctcgtcga tgtcggaatc cttcacgccc
     1561 ttggacttca ggaaggcacg caggatcttg ttggtggtgc gcgggtagac atagtcggtg
     1621 ccgagcagca cgaagcgctt ggcgccgccg ccttccttcg acatcaggta ttccaccgcg
     1681 gggatggcct gctggttcgg cgcggcgccg gtgtagaaca cgttgcgctc gagttcctcg
     1741 ccctcgtact gcaccggata gaacagcagg ctgttcagtt ccttgaacac cggcagcacc
     1801 gacttgcgcg acaccgaagt ccagcagccg aacaccaccg ccaccttgtc ctggctgatc
     1861 aactggcgcg cgcgctccgc aaacagcggc cagttggaag ccggatcgac cacgaccggc
     1921 tcgagcttct tgccaagcac accgcccttg gcattgatct cctcgatcgc catcagggcc
     1981 acatccttca gcgtggtttc ggagatggcc atggtgccgg acagcgagtg caggataccg
     2041 accttgatgg tgtcggatgc catggcgctc ggcatcattg ccgaggtcat gagggtcagg
     2101 gcggcggcgg cggtcgcctt gaggacggta cgacgagaca tggagagctc ctgagtagag
     2161 gtgggggcgt ggaatccggg tgcgattgct cgcaggaacc gttagagcac aagttgtgcc
     2221 accgcatgga atgcgtattc accatgcgga ctggagcaat gcagagttat ttcagtgcac
     2281 gccggcgcca cactggtgca gcaagagcaa gctttcgcga gaactcggtg cagtggcgcg
     2341 catttatttc atgcatgcac cgtatctgcg catggctcac aacgtgctta caggaacgcc
     2401 gctgttgctg ctcagccggc cccgtgcaca aggacgctga gcgcggcaac cggcgcatcc
     2461 atccgttctg gggcgccgcg acctaccgct cgatcaaata tgggaaggaa caccatgaag
     2521 aagaacacct gcgcacgcat ggcctggcca cgggccagcc tcgcactggc cgccctgctc
     2581 gccaccgcca gtgcccatgc ccatcccggc cacggcgagg gcgtcgtgtc cggactgctc
     2641 catccgctat tgggcatcga tcacctgctg gccatcctcg cgaccggcat ctgggcttcg
     2701 caagcaagct tggccggcca gcgcctgctg ccggccagct tcgtcggcgc gatgggactg
     2761 gcggcactgg ccgggttggc cggaatgccg ttgcaggccc tcgaacccgg catcgccgga
     2821 tcggtcctgc tggtcggcct gttgatcgcc tttcgcgtgc agatggcgcc ggccgccggc
     2881 gcgctggcag tcgccgtctt tgccgccttt cacggctacg cgcatggcgc cgagcttccc
     2941 gtcggcgcca gcgcctggca gtacttcgcc ggcttcctct tcaccacgct gctgctgctg
     3001 caggccggcc tgaaggccgg accgctgctg gcgcgctgga aagccgccct gccgaccacc
     3061 ggcgccgcgc tcgctgcggg cggcgcatgg atgctcggcc cgcttgcctg aaccacaagc
     3121 cgccacggcc gcccgtgcca cgccacggcc ccattgatag tagaatttcc ggctgtataa
     3181 gcttgggcgt agttgcgccg tcttcgcatg gaaactatgc ccaccgattc gtccgcgctt
     3241 tccctcctgc gccttgccac tgccccgctc cacacccgcc tggaacgcgg cttgaggatt
     3301 gtgcagccgg gagccggtca cgacgagttc cgcgcttacc tggtcgcact gtggggctgg
     3361 cactgcagct tcgaagaccg gctctggtcg tacgactggc ctgccccgct tgaagtcacg
     3421 cagcgccgtg ccaagttgcg ggccctcgcg caggatctgg aatcgctggg atttgacgaa
     3481 gaggcgcgcg atgcgctgcc gcgctgcgac tacgcgccgg cgctggacgg agtcgcagag
     3541 cgctatggcg tggcctacgt ggtcgaaggg gcccagctcg gcacgcgcgt actgggcaag
     3601 tcgcttgcac cggcgctcgc tccctggtcg cccgcctggc tggagggcta cggagccaac
     3661 gcgggcctga gctggaaaac gtttatagcc tgcctggaac gggcactgga gacacccgac
     3721 gcgcgcgcgc aagccgccgc cgccgcggtc ggcgcattcg aatcgttgaa tgtatggttt
     3781 gaactgtgcg gcagccggga gtgcacctgc gcttcctatc cgacgacgcc ggaccagaca
     3841 tgagtgacga tcccattgcc gcgccccaac ccgatcccgc actgtgtgcg caggaaccga
     3901 tccgcatccc cggcgccatc cagccgcacg gctttctgct cgccctgcac ccgagtgaac
     3961 acaccatcgt ccagctcagc gccaatgccg gccgcttcct cggcaggcaa gccggcgacc
     4021 tgatcggcgc gcgactggcc accgcggtgg gccaagacaa cgaggccgat ctgctcgatg
     4081 cgctctgccg tgcaagcggc aggcaaccct attacctcgg cgtggtgcgg ctcgagagcg
     4141 gcccgttcga cgtgctcggc caccaccatg acgcactgct ggtgctcgag ttcgagccgg
     4201 ccgcgcgcgc cgtgtcggca gatttccggc tgctttatcc cttgctcggc tccttcctcg
     4261 gcacgctcac cgcgaacgac tcgatcatga cgatgagcga acatgccgcc caggcgatca
     4321 agggggccac cggcttcggt cgtgtactgg tctaccgttt cgacgagtgc ggtcacggca
     4381 tcgtgctcgc cgaggcgcgc gacgatggtt atcactccta cctcaaccag cgctttcccg
     4441 cctcggatat tccgctgcag gcgcgcgaac tgtacctggc gaaccgtatc cgcctgatcc
     4501 cgaacgccga ctacgagccg gcgccgctga tcccggccga caatgccttg accggggcgc
     4561 cgaccgatct gtcctatgcg gcactgcgca gcgtctcacc catccatctg cagtacatgc
     4621 gcaatatggg cacactggct tcgatgtcgg tgtcgctggt ggtcaaaggt cggctgtggg
     4681 gattgatctc ctgccaccac gcgcatccgc gccacgttcc gttcgaagtg cgcacggggt
     4741 gcgaacaact ggcgcagata ctcgcgctgc gcatcgaatc gtgcgaagag cgggcgcaag
     4801 cgcatcaccg gctcgaactg cgcagcctgc tcgtggccat gctggcgggg ctgacgcgag
     4861 gcccggattt catcgacaac atcacagcga tttcgccgca gttgctgcgc tttgccagcg
     4921 ccagcggggc cgccgtcgtg tttgaacagc gcgtcgtgcg cttcggcgac gccccgcccg
     4981 aggccgacat tcacaagatc gtcgaatggc tggccgcgcg tccgcacgac gaggccttcc
     5041 acaccgatgc gctggcgcag ttgatgccgg aagcggcatc gatggcccgc cacgccagcg
     5101 gcattctggc cctgccgatt tccagcctgc accggcatta cctcatctgg ttccgtccgg
     5161 agatcgtgca taccatcgat tgggcgggca atccgcacct caaagacgag gccggcgccg
     5221 tgccgtcggc tgtgctgaca ccgcgcgcca gtttcgacag ctggcgcgaa accgtgcgcg
     5281 gccgctcggc cgcctggcgc ccgagcgaga tcgagtgcgc aaacgaattc cgtgccgcgc
     5341 tcctgggcgt ggtgctggag cgcgcggagc agatggccga gctggccgag gagctcgggc
     5401 gcgccaacaa ggagctggaa tctttctcct attcggtgtc gcacgatctg cgcgcgccgc
     5461 tacgccacat cgccggttac agcgatctgc tgctcgaact ggaggaaggc caactgtccg
     5521 aacgcggcca gcggtacgtc cgcaatatca aggactcggc ccgctttgcc ggcaaactgg
     5581 tcgacgacct gctcagtttc tcgcaaatgg gacgcgcctc gctgcgccca ggcacagtcg
     5641 acctgaacga attggtcgca tcctgtatcg aacatctcgc cgacgaggcc aaggggcggg
     5701 aactgcactg ggaagtcagt ccgctgccca tcgtccaggc agacccgcgc tttctgttgc
     5761 tggcgctgcg caacctgctc tccaatgccg tcaagtacac gcggccgaaa ccccaggcgc
     5821 ggatttcgat cagcgcgcac agctcggacg aggaaaccgt tgtgcaagtg cgcgacaatg
     5881 gggtcggctt caataccgag tacaagcaca agctgttcgg cgtttttcag cgcctgcacc
     5941 gttacgagga attcgaagga acgggaatcg gcttggcgaa cgtgcaacgc attgtcgaac
     6001 ggcacggcgg ccgcgtatgg gccgatagcg taatcgggga aggcgcggta ttcaccttct
     6061 ccctcccgaa acaactaccc actgactaga cgctccatgc tcaaacccat cctgctggtc
     6121 gaagacaacc caaacgatct ggagctgaca ctgatcgcgc tggaaaaaag caagctcgcc
     6181 aacgacgtgg tggtggtgcg cgacggcgcc gaggccttgt cgtacctgaa cgccagcggc
     6241 gactatgcgc accgaccggc gggcaatccc gccgtgatcc tgctcgacct gaagttgccg
     6301 aaggtagacg gcctggaagt gctgcgccat atccgcgaga cgccgccgtt gaagagcata
     6361 ccggtggtga tgctcacttc ctcgcgcgag gagcaggacc tgctgcgaag ctacgaactc
     6421 ggcgtcaacg cctatgtggt caagccggtg gagtttagtg aattcgtgca ggcgatcgcc
     6481 gatctcggga tcttctgggc ggtgctgaac gaaccgccgc cggggtcgat gcgctacatg
     6541 ccgcgtccct aaggttcccg acgcgtttca ttctccctcg ccgacacgct cgggctcgag
     6601 catttgcgcg atttcccgcg ccagcgcctc gccgccgtac ggcttcttca gcagccggat
     6661 atgcgcctcc agcttgccgt cgcgcgccag ctccccttcc ggataacccg aagtgaacaa
     6721 cacctgcagg cgcggcagtt tctggcgcgc gtgcttcacc aattcgccgc tgcgcaccgt
     6781 ccctggcatg atcacatcgg taaacagtag atcgaccacc agcccgcttt cgatcaaggc
     6841 caacgcggtc gccccgtcct gggcctggaa taccgtgtag ccgagcccgg acaggaacac
     6901 caccgcagtc tcgcgcacct cggcttcatc ttcgacgacc agaatgctgc cgcgc
//
LOCUS       DATLGF010000330         4723 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_30067,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000330 DATLGF010000000
VERSION     DATLGF010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30067"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VM406_10090"
     CDS             <1..207
                     /locus_tag="VM406_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL76357.1"
                     /translation="TIINLVTAGVGLALLREDVALSVAASNELVLWENGSRKSTLSFV
                     HLKERGDEPAIAGATEIVREVWVG"
     gene            complement(240..1151)
                     /locus_tag="VM406_10095"
     CDS             complement(240..1151)
                     /locus_tag="VM406_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVL76358.1"
                     /translation="MKIRQLEAFRALVIGQTVTQAARMLYISQPAVTRLIADLEQDVG
                     FALFERKRGRLYPTAEGLALYEEVERSFIGMERIQSTAAGIKASSVGMLQIAAAPALS
                     LSFLPRAISAFIRNHPDVNFSLTSNTSRAVVDMVMKQRCSVGFVILPMNYPSMHGELL
                     INTDLLAAMPAGHRLADKEVITPTDFDGERYVSNPRDLQSRLQADALFASFGVERKLQ
                     IETQINAGVCAFIEAGLGVALVDPVTALDYIGRGVVFRRFEPTLQTEFQVLYPSERPP
                     SILVRAFVSHVRRFLQAELEERIYRRI"
     gene            1303..2331
                     /locus_tag="VM406_10100"
     CDS             1303..2331
                     /locus_tag="VM406_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL76359.1"
                     /translation="MAIKQLALAAMLAAGVTAAHAQQTLYLGAYGGSTEKLFKEKIIP
                     AFEAKHNVKIVYVTGNSTDTLAKLQAQRARQELDVVLLDDGPMYQAMQFGFCDKVQAA
                     PVYNDLYSLARMGDNAVAVGVVATGLAYNPEAFKKAGLAAPDSWEILTDKRFRQKVAI
                     PPISNTYGLQTLTMFAKLRGGGDTNIEPGFAAMTKEVAPNVLAWEPSPGKMTELFQNG
                     DVTLAVWGSGRVQALKDTGFPVEFVYPKEGALALMVAACPVVQSDVSAQSQAFIQYLL
                     TPEVQAMLADSQGWGPTNKKANLSPAVAAKVPYGEQQVGKLIATNWSAVNEKRAEWTN
                     RWNRTVER"
     gene            2452..3519
                     /locus_tag="VM406_10105"
     CDS             2452..3519
                     /locus_tag="VM406_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL76360.1"
                     /translation="MTFLTLDSLAKRFGDFTAVESMSLEIERGEFLSLLGPSGCGKTT
                     TLQMVAGFVEPTRGRILVDGKDITRLRPEKRGMGIVFQSYALFPHMTVGENVGFGLEM
                     RGMPRAEREKRIAETLELVRLNGLDKRYPKELSGGQRQRVALARALAIRPHVLLLDEP
                     MSNLDAKLREEMHIELRAIQRHLGITTILVTHDQAEALTMSDRIAVMYGGRIVQLGTP
                     VEAYERPESPFASAFLGKINSIPGLVLARHDHCCEVRLGDSIARVPHEGREVGTAVNV
                     YVRPEKVRLQAPGSGGLSGRVKTRVFLGSYWMLEVETVLGLLRASIPNSGITPPAEGE
                     MVALAWADEDLRVLSAETAHG"
     gene            3512..4387
                     /locus_tag="VM406_10110"
     CDS             3512..4387
                     /locus_tag="VM406_10110"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL76361.1"
                     /translation="MASTVENRSTAAPWLLSAPALLLFVGLLLVPLVLTAILSFRVFD
                     GTQGVMPEYTLAHYWEVFSDDYYHEIFLRTGGLALAVTLLSIVFGVPETIILARMKSP
                     WRGLFLVVILGPLLISVVVRTLGWAILLGNNGLINTALQALGITDEPIRLLFTMTGVT
                     IALTHVLIPFMVIAVWAALQKLDPQVENAGLSLGASPFTVFRRVILPQILPGILSGGI
                     IVFALAASAFATPAILGGRRLKVVASAAYDEFLSTLNWPLGATIAVLLLIANVVIILG
                     CNRLIERRYRTVFES"
     gene            4398..>4723
                     /locus_tag="VM406_10115"
     CDS             4398..>4723
                     /locus_tag="VM406_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL76362.1"
                     /translation="MRKNGFFSLLYHALFIGFITAPLLIVIAVSFTDKGYISLPTDGL
                     SLRWFYAILDATDIVNAFWMSLWLGLASASVAVVLAVPGALALSRYRFPGRGALMAFF
                     MSPLMIP"
BASE COUNT          820 a         1544 c         1511 g          848 t
ORIGIN      
        1 acgatcatca atctggtcac ggcaggcgtc ggcctggcgc tgctgcgcga ggacgtggcc
       61 ctgagcgtgg cggcgtcgaa cgagctggtg ttatgggaga acggatcgcg gaaatcgacc
      121 ttgtcgttcg ttcatctgaa ggaacgcggg gatgagccgg cgatcgccgg cgcgaccgag
      181 atcgtgcggg aggtctgggt ggggtgaggt tttggcgcgg tccagtcacc gcgaccgcat
      241 caaatccgcc gataaatcct ctcctccagc tccgcctgca ggaacctgcg cacgtgcgac
      301 acgaacgcgc gcacgaggat cgacggcggc cgctccgacg gatacaacac ctggaattcg
      361 gtctgcagcg tcggttcgaa gcgcctgaac acgacgccgc ggccgatata gtcgagcgcc
      421 gtgaccgggt ccaccagcgc gacgcccagc cccgcctcga tgaatgcaca cacgccggca
      481 ttgatctggg tctcgatctg cagcttgcgc tcgacgccga atgatgcaaa caaggcatcg
      541 gcctgcaggc gcgactgcag gtcgcgcggg ttcgatacat agcgctcgcc gtcgaagtcg
      601 gtcggcgtga tcacttcctt gtcggcgagt cggtgcccgg caggcatggc cgccagcagg
      661 tccgtgttga tcagcagctc gccgtgcatg ctcgggtagt tcatcggcag gatcacgaaa
      721 cccacgctgc agcgctgctt catcaccatg tccaccaccg cgcgcgaggt attgctcgtt
      781 agcgaaaaat tcacatcggg gtgattgcgg atgaacgccg agatcgcgcg cggcaggaac
      841 gacagcgaca aggccggcgc ggcggcgatc tgcagcatgc cgacgctcga cgccttgatt
      901 ccggcggcgg tgctctggat gcgctccatc ccgatgaacg agcgctcgac ctcttcgtac
      961 agcgccaggc cctcggcggt cgggtacagc cgcccgcgct tgcgctcgaa cagcgcgaaa
     1021 ccgacatcct gttccaggtc ggcaatcagc cgggttaccg ccggctgcga aatgtagagc
     1081 atgcgcgcgg cctgggtgac ggtctggccg atgacgagcg cacgaaaggc ttcaagctgg
     1141 cggatcttca tgggccgcaa ttctataacc caatgttatg cgcgaccgaa aaaaagtgat
     1201 ttttctttcc cgaattccgg aattagtctt tgttgcaccc ggaacttgtc atcaagtcgt
     1261 cataaaagct cgggacctca atcaacttgt tggagtgaac acatggcaat caagcaactg
     1321 gccctggccg cgatgctggc cgccggcgtc accgccgcgc atgcacagca gaccctctat
     1381 ctcggcgcct atggcggctc gaccgagaag ctgttcaagg aaaagatcat cccggcgttc
     1441 gaggccaagc acaacgtcaa gatcgtttac gtgaccggta actccaccga cacgctggca
     1501 aagctgcagg cccagcgcgc ccgtcaggaa ctggacgtgg tgctgcttga cgacggcccg
     1561 atgtaccagg caatgcagtt cggcttctgc gacaaggtcc aggccgcgcc ggtctacaac
     1621 gacctctaca gcctggcccg catgggcgac aacgcggtcg ccgtcggcgt ggtcgcgacc
     1681 ggcctcgcct acaatccgga agccttcaag aaggccggcc tggccgcccc ggattcgtgg
     1741 gaaatcctga ccgacaagcg tttccgccag aaggttgcga ttccgccgat ctccaacacc
     1801 tacggcctgc agacgctgac catgttcgcc aagctgcgcg gtggcggcga caccaacatc
     1861 gagcccggct ttgccgcgat gaccaaggaa gtcgcgccga acgtgctggc atgggaaccc
     1921 tctccgggca agatgaccga gctgttccag aacggcgacg tgaccctcgc ggtatggggc
     1981 agcggccgcg tgcaggcgct gaaggacacc ggcttccctg tcgagttcgt gtatccgaag
     2041 gaaggcgcgc tggcgctgat ggtcgccgcc tgcccggtgg tgcagagcga cgtgtcggcg
     2101 cagtcccagg cattcatcca gtacctgctc acgcctgaag tccaggcgat gctggccgac
     2161 agccagggct ggggtccgac caacaagaag gccaatctgt cgcccgccgt cgccgccaag
     2221 gtgccgtacg gcgaacagca ggtcggcaag ctgatcgcaa ccaactggag cgcggtcaac
     2281 gagaagcgcg ccgagtggac caaccgctgg aaccggacgg tggagcgtta agcaaccgta
     2341 gcgctggagg tggcatgcgc ggcgccagcc gtctctgtcg cctccttcaa tgcgacacca
     2401 tccacagtgt cgctcccgcg gcttgcatca tctacacccg agatctaccc catgacattc
     2461 ctgaccctgg acagcctcgc gaagaggttt ggagatttca cggccgtcga aagcatgagc
     2521 cttgagatcg aacgcggcga attcctctcc ctgctcggcc cgtccggctg cggcaagacc
     2581 accacgctgc agatggtggc cggtttcgtg gagccgaccc gcggacgcat tctcgtggac
     2641 ggcaaggaca tcaccaggct gcgtccggaa aagcgcggca tgggcattgt gttccagagc
     2701 tacgcgctgt tcccgcacat gacggtgggc gagaacgtcg gtttcggcct ggagatgcgc
     2761 ggcatgccca gggccgagcg cgagaagcgc atcgccgaga cgctggaact ggtgcgcctg
     2821 aacggtctcg acaaacgtta tccgaaggaa ttgtccggcg gccagcgcca gcgcgtcgcc
     2881 cttgcccgtg cgttggcgat ccgcccgcac gtgctgctgc tggacgaacc gatgtccaac
     2941 ctcgacgcca agctgcgcga ggaaatgcat atcgagctgc gcgcgatcca gcgccacctc
     3001 ggcatcacca ccatcctggt cacgcacgac caggccgagg cgctgacgat gagcgaccgc
     3061 atcgccgtga tgtacggcgg tcgcatcgtc cagctcggca ccccggtgga agcctacgag
     3121 cggcctgagt cgccgtttgc gtcggccttc ctcggcaaga tcaattcgat tccgggcctc
     3181 gtgctggcgc gccacgacca ctgctgcgaa gtgcggctcg gcgattcgat tgcgcgcgta
     3241 ccccatgaag gccgcgaagt cggcaccgcg gtcaacgttt atgtgcgccc ggagaaggtg
     3301 cgcctgcagg cgcccggatc cggcggcctg tccggacgcg tgaagacgcg cgtgttcctg
     3361 ggcagctact ggatgctgga agttgaaacc gtgctgggcc ttttgcgcgc aagcattccg
     3421 aattccggca tcaccccgcc cgccgagggc gagatggtgg cgttggcctg ggccgatgaa
     3481 gacctgcgcg tgctgtcggc ggagactgca catggctagc acggttgaaa accgcagtac
     3541 cgccgccccc tggctgctgt cggcgccggc cctgctgctc ttcgtcggcc tgctgctggt
     3601 gccactggtg ctgacggcca tcctgtcgtt ccgggtgttc gacggcacgc agggcgtgat
     3661 gcccgagtac acgctggccc attactggga agtgttctcc gacgactact accacgagat
     3721 cttcctgcgc accggcgggc tggcgctggc ggtgacgcta ctgtcgattg tgttcggcgt
     3781 gcccgagacc atcatcctcg cgcgcatgaa gagcccctgg cgcggcctgt tcctggtcgt
     3841 gatcctcggt ccgctgctga tctcggtggt ggtgcgcacg ctaggctggg cgatcctgct
     3901 cggaaacaat ggcctgatca acaccgcgct gcaggcgctg ggcattaccg acgagccgat
     3961 ccggctgctg tttacgatga ccggcgtgac gatcgccctc acccacgtgc tgattccgtt
     4021 catggtgatc gcggtctggg cggcgctgca gaagctcgat ccgcaggtcg agaacgccgg
     4081 gctttcgctg ggcgcctcgc cgttcacggt attccgccgc gtgatcctgc cgcagatcct
     4141 gccgggcatc ctgtcgggcg gcatcatcgt gttcgcgctg gcggcatcgg ccttcgcaac
     4201 cccggccatc ctcggcggac ggcgcctgaa ggtggtcgcc tcggcggcct acgacgagtt
     4261 cctgagcacg ctgaactggc cgctcggcgc caccatcgcg gtgctgctgc tgatcgcgaa
     4321 cgtcgtgatc atcctcggct gcaaccgcct gatcgagcgg cgctatcgca ccgtgttcga
     4381 atcctgaagg agaccccatg cgcaagaacg gatttttctc gctgctctat cacgcgctct
     4441 tcatcggctt catcaccgcg ccgctgctga tcgtgatcgc cgtgtcgttc accgacaagg
     4501 gctacatctc gctgccgacc gacggcctct cgctgcgctg gttctacgcg atcctggacg
     4561 cgaccgacat cgtcaacgcg ttctggatgt cgctgtggct ggggctggct tcggccagtg
     4621 tcgcggtggt gctcgcggta cccggcgcgc tggcgctgtc gcgctaccgc tttccgggcc
     4681 gcggcgcgct gatggccttc ttcatgtcgc cgctgatgat ccc
//
LOCUS       DATLGF010000331         4974 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_213011, whole genome shotgun sequence.
ACCESSION   DATLGF010000331 DATLGF010000000
VERSION     DATLGF010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213011"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /gene="rimO"
                     /locus_tag="VM406_10120"
     CDS             complement(<1..496)
                     /gene="rimO"
                     /locus_tag="VM406_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141109.1"
                     /note="catalyzes the methylthiolation of an aspartic acid
                     residue of the S12 protein of the 30S ribosomal subunit;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HVL76363.1"
                     /translation="MSTNPKVGFVSLGCPKALVDSEQILTQLRAEGYETAKSYDGADL
                     VIVNTCGFIDAAVQESLDAIGEALAENGKVIVTGCLGAKKDINGNDIIQSVHPKVLEV
                     TGPHAVGEVMSAVHKHLPKPHDPFVDLVPAQGIKLTPKHYAYLKISEGCNHRCTFCSI
                     PSMRG"
     gene            complement(573..1133)
                     /gene="phaR"
                     /locus_tag="VM406_10125"
     CDS             complement(573..1133)
                     /gene="phaR"
                     /locus_tag="VM406_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HVL76364.1"
                     /translation="MNSAKKISERLIKKYPNRRLYDTQTSSYITLADVKQLVLDNEEF
                     TVVDAKTEEDLTRSILLQIILEEEANGTPMFSSSALSQIIRYYGHAMQGMMGSYLEKN
                     IQAFIEIQNKLAENSKGFYEGKPFSPEMWTQFMNVQGPMMQGMMSNYIEQSKNLFLQM
                     QEQMQNQTKTMFGSFPFPPNDQNHKK"
     gene            complement(1293..3008)
                     /gene="phaC"
                     /locus_tag="VM406_10130"
     CDS             complement(1293..3008)
                     /gene="phaC"
                     /locus_tag="VM406_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HVL76365.1"
                     /translation="MNMFQPQAEQAAWTPPKIDPAALQALLHAAPIDTRPFTAAMKEF
                     SANVDPSVLAELQADYMRQLSSLWSDMLASKTPTIADRRFGSPEWQSNPLSAFHAAVY
                     LLNARFLNALADAVQASPRARQKIKFAVQQMVDAMSPANFLVSNPEAQQKLIETKGES
                     LAKGIAHMLEDMKKGRISQSDESAFEVGRNVANTEGAVVYENELFQLIQYKPLTKSVY
                     QRPLLLVPPCINKYYILDLQPDNSLVRHAVEQGHSVFLVSWCNPDASLGHLTWDDYIG
                     QGTLEAIRVTQEISGQEQLNMLGFCVGGTILASALAVLAARGEHPAASLTLLTTLLDF
                     SDTGVIDVFIDEAQVAMRERTIGKGGLMPGRDFASAFSSLRPNDLVWNYVKTNYLKGE
                     TPPPFDLLYWNSDSTNLPGPMFCYYLRNMYLENNLRKPGRLTVLGENVDLSLIDAPVF
                     VYGSREDHIVPWAAAYESTALLNPRRRKRNRFVLGASGHIAGVINPPAKKKRSYWVNE
                     EPALTAEAWLAGAAEHPGSWWPEWTAFLKQNAGKQVAAPKRLGNAKYKPIEPAPGRYV
                     KVKAE"
     gene            3060..3302
                     /locus_tag="VM406_10135"
     CDS             3060..3302
                     /locus_tag="VM406_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76366.1"
                     /translation="MYIVALAWTYVVLMMSITETSVVAGVMTFLFYGAIPVSIILYVM
                     GTPQRRRRRLLAEKIREEEAAEAKRMAAGQTGEPGA"
     gene            complement(3320..4075)
                     /gene="pgeF"
                     /locus_tag="VM406_10140"
     CDS             complement(3320..4075)
                     /gene="pgeF"
                     /locus_tag="VM406_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HVL76367.1"
                     /translation="MNGSFLVPEWHAPAGVAALSTTRRGGVSRAPYDDGEGGGGLNLG
                     AHVGDDPDSVRANRALLRTRLPDEPAWLSQVHGNRVVHADSAVDAPQADASITSRRGV
                     VCAIQTADCLPVLFCDVAATVVGAAHAGWRGLAGGVLQNTVGAMRTAGARDIIAWLGP
                     AIGPRRFEVGQDVVKAFCAQSPGLRSAFVPREAVPGKHLADIYALARMILAEAGVRQV
                     FGGGRCTVEESRDFYSYRRDGVTGRMASLIWLK"
     gene            complement(4072..>4974)
                     /locus_tag="VM406_10145"
     CDS             complement(4072..>4974)
                     /locus_tag="VM406_10145"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HVL76368.1"
                     /translation="GVRLDKVLSQLLPQYSRSRLQQWIEAGCVSIDGRPARAKMQVLG
                     DETITVLPQEVPEEGAYEPQPMALSILFEDEAILVLDKPAGLVVHPAAGNWSGTLLNG
                     LLHHAPALAGVPRAGIVHRLDKETSGLMVVAKTLEAQTALVRQLQARTVTREYLALVW
                     GAPQLSGHVDAPIGRHPRDRVRMAVSRHAGAKPAVTHYQRVATGFLNEQPVALLRCRL
                     ETGRTHQIRVHMQSIGFPLVGDQVYGKPHLAAVFPRQALHAHRLAFEHPATHADCAWQ
                     ADVPGDIAALLKRAGIAAVEGEWR"
BASE COUNT          854 a         1594 c         1560 g          966 t
ORIGIN      
        1 cgccgcgcat cgacgggatg ctgcagaacg tgcagcggtg attgcagccc tcggagatct
       61 tcaggtacgc gtagtgtttc ggcgtgagct tgatgccctg ggccggaacc aggtcgacaa
      121 aaggatcgtg cggtttcggc aggtgcttgt gcacggctga catcacctcg ccgaccgcat
      181 gcgggccggt cacttcgagc accttcgggt gcacgctctg gatgatgtcg ttaccgttga
      241 tgtccttctt cgcgcccagg cagccggtga cgatgacctt gccgttctcg gccagcgcct
      301 cgccgatcgc atcgagcgat tcctgcacgg cggcgtcgat gaagccgcag gtattgacga
      361 tcacgaggtc ggcgccatcg tagctcttgg cggtttcata gccttcggcg cgcagctggg
      421 tcaggatctg ttccgaatcg accagcgcct tcgggcagcc gagcgatacg aaaccgactt
      481 tgggattggt ggacataaag gaaaaagggg cagaacgacg aatccgccat tctacccctt
      541 gtcgcatcct gcgccgcacc ggcgcggccg cgtcacttct tgtggttctg atcgttcggt
      601 gggaacggga agctgccgaa catggtcttg gtctggttct gcatctgctc ctgcatctgc
      661 aggaagagat tcttgctctg ctcgatgtag ttgctcatca tcccctgcat catcgggccc
      721 tgcacgttca taaactgggt ccacatctcc gggctgaacg gcttgccttc gtagaagccc
      781 ttcgaattct ccgccagctt gttctggatt tcgatgaagg cctggatgtt cttctccagg
      841 taggacccca tcatgccctg catcgcatgg ccgtagtaac ggatgatctg cgacagcgcg
      901 ctgctggaaa acatcggggt gccgttggcc tcctcttcca ggatgatctg cagcaggatg
      961 ctgcgggtca ggtcttcctc ggtcttcgca tcgacgaccg tgaactcttc attgtcgagg
     1021 acgagctgct tgacgtcggc aagggtgatg taggaactgg tctgggtgtc gtacaggcgg
     1081 cggttcgggt acttcttgat caggcgctcg gaaatctttt ttgcactatt catcgaaaat
     1141 cccggtcact gcgtcgcaac gcatcgaggt tgaactgagg cgaagtgaac atccgttcgt
     1201 ccaaatcgcc tccaattata gttcggaaaa acagggattt acgcaatttc ggaccgcatt
     1261 tcccgcaccg gttcaccgat ccttcgtccc gatcactccg ccttgacctt cacataacgc
     1321 cccggcgccg gctcgatcgg cttgtacttc gcattgccaa ggcgcttggg cgccgccacc
     1381 tgcttgccgg cgttctgctt gaggaaagca gtccattccg gccaccagct tcccggatgc
     1441 tcggccgccc cggcaagcca ggcttcggct gtcagcgcgg gttcttcatt gacccagtag
     1501 ctacgcttct ttttggcagg cggattgatc acgccggcaa tgtggccgga tgccccgagc
     1561 acgaagcggt tgcgcttacg ccgccttgga ttcagcagcg cagtcgattc atacgcggcc
     1621 gcccaaggca cgatatggtc ttcgcgcgag ccgtagacga acaccggcgc atctatcagc
     1681 gacaggtcga cgttttcccc cagcaccgtc agcctgccag gcttcctgag attgttctcg
     1741 aggtacatat tgcgcaggta gtagcagaac atcggtcccg gcaggttggt gctatcgctg
     1801 ttccagtaca gcaggtcgaa cggcgggggc gtctcgccct tcaggtagtt cgtcttcaca
     1861 tagttccaca caaggtcgtt cggacgcagg ctggagaacg ccgaggcaaa atcgcgtccg
     1921 ggcatgagtc cgcccttgcc gatcgtccgc tcgcgcattg cgacctgggc ttcgtcgatg
     1981 aagacgtcaa ttacgccggt gtcggaaaaa tcgagcagcg tggtcaacag cgtaagactg
     2041 gccgccgggt gctcgccgcg cgcagccagc accgcgagcg cactggcgag gatcgtgccg
     2101 ccgacgcaga agccgagcat gttgagctgt tcctgtcccg aaatttcctg cgtgacacga
     2161 atcgcctcca gcgtgccctg gccgatgtaa tcgtcccagg tgagatggcc gaggctggcg
     2221 tccgggttgc accaggagac aagaaacacc gaatggccct gctccaccgc gtggcgcacc
     2281 agcgaattgt cgggctgcag atcaaggatg tagtacttgt tgatgcatgg cggcaccagc
     2341 agcagcgggc gctggtagac cgacttggtc agcggcttgt actggatcag ctggaacagc
     2401 tcgttctcgt acaccaccgc cccttcggtg ttggcgacgt tgcgcccgac ttcgaacgcg
     2461 ctttcgtcgc tctgcgagat gcgccctttc ttcatgtctt ccagcatgtg ggcgataccc
     2521 ttggccaggc tctcgccctt ggtctcgatc agcttctgct gtgcttccgg attgctgacc
     2581 aggaagttcg ccggcgacat cgcatcgacc atctgctgca ccgcgaactt gatcttctgc
     2641 ctggcgcgcg gggaagcttg caccgcgtcc gcgagcgcat tgaggaagcg cgcattgagc
     2701 aggtagaccg cggcgtggaa ggcgctgagc gggttcgact gccactccgg ggacccgaag
     2761 cgacgatcgg cgatcgtcgg cgtcttcgat gccagcatgt cgctccagag actcgagagc
     2821 tggcgcatgt agtccgcctg caactccgcc aacaccgacg gatcgacgtt ggcgctgaat
     2881 tccttcatcg cggcagtgaa gggccgggtg tcgatcgggg ccgcatgaag cagcgcctgc
     2941 agcgcggcag ggtcgatctt ggggggcgtc catgcggcct gctcggcctg gggctggaac
     3001 atgttcatgc tggcttctcg cttttcgcgt atggttacgg tttgctcgaa aggtgagtca
     3061 tgtatatcgt tgccctggcc tggacctatg tcgtgctgat gatgtccatt accgaaactt
     3121 cggtggtcgc gggcgtgatg acatttttgt tctacggtgc gattcccgtg tcaatcattc
     3181 tatacgtgat gggaacgccg cagcgcaggc gcaggcgcct gctcgccgaa aaaatccgcg
     3241 aggaggaagc ggccgaggcg aagcggatgg ccgccggtca aaccggcgag ccgggagcat
     3301 agcgcgcgtt acagcgggtc tacttgagcc agatcaggct cgccatgcgg ccggtgacgc
     3361 cgtcgcgccg gtacgaatag aagtcgcgcg actcctcgac ggtacagcgt ccgccgccga
     3421 acacctgcct cacccccgct tcggcaagga tcatgcgcgc aagtgcatag atgtccgcaa
     3481 gatgcttgcc cggcaccgcc tcgcgcggga cgaacgcgga ccgcagcccg ggcgactgcg
     3541 cgcaaaacgc tttcaccacg tcctgtccca cctcgaaacg gcgcgggccg atcgccggac
     3601 cgagccaggc aatgatgtcc ctcgctccgg ccgtgcgcat cgcgccgaca gtgttctgca
     3661 acaccccgcc ggccagcccg cgccagcccg catgggcggc gcctaccacc gtagccgcga
     3721 cgtcgcagaa cagtaccgga aggcagtcgg ccgtctggat cgcacacact acgccgcgac
     3781 gactcgtaat gctggcatcc gcctgcggcg cgtcgactgc ggaatcggcg tgcacaacgc
     3841 ggttgccgtg cacctgcgac agccaggccg gctcgtccgg aagccgggtg cgcaacagcg
     3901 cccggttggc ccgcacgctg tccggatcgt cgccgacatg cgcccccaga ttcagccccc
     3961 ctcctccctc gccatcgtcg taaggcgcac ggctgacgcc gccgcgccgg gtggtggaca
     4021 gtgctgccac gcccgcaggc gcgtgccact cgggaaccag aaagcttccg ttcatcgcca
     4081 ttcgccttcg actgcggcga tgcctgcccg tttcagcagc gccgcaatgt cccccggcac
     4141 gtcggcttgc catgcgcagt ccgcgtgagt ggccgggtgc tcgaaagcaa gacggtgcgc
     4201 gtgcaaggct tggcgcggaa acaccgcggc cagatgcggc ttgccataga cctgatcacc
     4261 gaccagcgga aagccgatcg attgcatgtg cacgcggatc tggtgggtgc ggccggtttc
     4321 gagcctgcaa cgcagaagcg caaccggctg ctcgttgagg aagccggtgg caacgcgctg
     4381 gtagtgggtg acggccggct tggcgccggc atggcgcgac accgccatcc gcacccggtc
     4441 gcgtggatgc cggccgatcg gcgcgtcaac atgaccggac aactgcggcg cgccccagac
     4501 cagcgccagg tattcacgcg tgacggtgcg tgcctgcaac tggcgcacca gcgcggtctg
     4561 ggcctccagt gtcttggcca ccaccatcag tccgctggtc tccttgtcga ggcgatgcac
     4621 gatgccggcg cgcggtacgc ccgccagtgc gggggcgtga tgcaacagtc cattgagcag
     4681 ggtgcccgac cagtttcccg ccgccggatg gacgaccagc cctgccggtt tgtcgagcac
     4741 caggatggcc tcgtcctcga ataggatgga cagcgccatc ggctgcggct cgtaggcgcc
     4801 ctcctccggc acctcctgcg gcagcacggt gatggtttcg tcgcccagca cctgcatctt
     4861 ggcgcgcgcg ggccggccgt cgatgctcac gcaaccggcc tcgatccact gctgcagccg
     4921 gctgcgcgag tattgcggca gcagctgcga caggaccttg tcgaggcgca cgcc
//
LOCUS       DATLGF010000332         4318 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_139559, whole genome shotgun sequence.
ACCESSION   DATLGF010000332 DATLGF010000000
VERSION     DATLGF010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139559"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VM406_10150"
     CDS             complement(<1..735)
                     /locus_tag="VM406_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVL76369.1"
                     /translation="MDSLITASARALAAGDPLGALKRVALRDDPPALALRGIAMAQLG
                     EHVRARELLRRAARAFGAHEERSRARCVVAEAEVALAMRDLGGSPRVLAAAAATLDAH
                     ADRCNALHARLIATRRLLLLGRLDEAAAALAQLDVRGMPPALVAVAELTAAELALRSL
                     HIAAARTALAHATDACERAGVPALQAEVTELRAMLDRPAARRVGAGGEQPLRLDEVAA
                     LLASDVLVVDACRRRFGSGATWRSLAR"
     gene            888..1352
                     /locus_tag="VM406_10155"
     CDS             888..1352
                     /locus_tag="VM406_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HVL76370.1"
                     /translation="MSHAARIALHKHAPALLDAQLKLSNAAKQSSLGERLVELINLRI
                     SQINGCGFCVDMHWRDLLRQGVDPRHLNAVAVWREAPFFSERERAALRWAELVNGQPH
                     VEASDAEFARVREFFSETEVVELGYAVAAIKGWNMMNRSLQNPIPEVPAPGM"
     gene            1375..2271
                     /gene="sigJ"
                     /locus_tag="VM406_10160"
     CDS             1375..2271
                     /gene="sigJ"
                     /locus_tag="VM406_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
                     /protein_id="HVL76371.1"
                     /translation="MNETEAGGREAVFAGLRPRLFSAAYRMLGTRADAEDVVQDAWLR
                     WQAADHAALRSPEAWLVTVTTRLALDRLRERKAERESYTGWWLPEPLVELDENTPETA
                     AELASDVSFALLWTLERLGAEERAAFLLRQAFDQDYADIAALLGKSEASCRQLVHRAQ
                     EKLRDSRPRFQPAPEAHRALLAAFTEAARRADREAMKALLSPDVSFVSDGGGKMPSFH
                     RVLRGAGRIAGLFWWLEQQHPGKVQYRLARINGGPGLLRYVDGRIESAQSFHFEDGQI
                     AAVFVVRNPDKLAGVPQRLRSW"
     gene            2633..>4318
                     /locus_tag="VM406_10165"
     CDS             2633..>4318
                     /locus_tag="VM406_10165"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVL76372.1"
                     /translation="MQEPFNPMAEQNALGILWLNEAGCITYANDAVAHLISTGTDTLV
                     GKPIKELIVELHPSAWVELATSISPGESRLLQVTLHKAGHGSAAAEAHVLRLSDKGNR
                     SFAVFLQGIATPVRTEELFRLQHDVIEALARDEPLAKLLNRLCLRIEAIARDLLCSVI
                     LVDEHDRVHVVAAPSIPKGYSIAIDGQPIGPKAGSCGTALYFNQPVEVSDIAHDPLWE
                     NYRQLVLPIGLVACWSQPITARDGRVLGSFAIYYRKPRGPSEFHRQLVEVCTHLTAIA
                     IERTRMDERLHDLAFHDVLTKLPNRKLLADRGAIALANAAHAQHPVALLHIDLDRFKR
                     INDAHGHAVGDRFLQAYADQLQAAVRKGDTVSRVGNDEFVLVLPRCDGQQAAHLAQRT
                     LDILAEHVILGQKRFVASASIGISLYPDDAKDFQTLLRYAEMAAHQVKKGGGNGYRFF
                     SRELNAANQELAALEQALRGALAARRLSVHYQPKMDLNRESVYGIEALARWNDPQLGM
                     VPPARFIPVAEASGLIHELGRWVLDEACRQLAEWRAEELAIPYVEVNVSAKQLG"
BASE COUNT          764 a         1428 c         1417 g          709 t
ORIGIN      
        1 ccgtgccagc gagcgccacg tggccccgct tccgaacctg cgcctgcagg catcgaccac
       61 cagcacatcc gaggccagca gcgccgccac ctcgtccagg cgcaatggct gctcaccacc
      121 ggcgccgacg cgcctggccg cgggccgatc gagcattgcc cgcagctccg tcacttccgc
      181 ctgcaaggcc ggcacgcctg cgcgctcgca ggcatcggtc gcatgcgcga gcgccgtgcg
      241 cgcggcggcg atgtgcaacg agcgcagcgc cagctcggcc gccgtcagtt cggccacggc
      301 caccagcgcc ggcggcatcc cgcgcacatc gagctgcgcc agcgccgccg cagcttcatc
      361 gagccggccc agcagcaaga gccggcgcgt ggcgatcagg cgtgcgtgca gcgcgttgca
      421 gcggtcggca tgggcatcga gcgtggccgc ggcagccgcc agcacgcgcg gcgagccgcc
      481 gagatcgcgc attgccagcg ccacctcggc ctccgccacc acgcagcgcg cacgcgagcg
      541 ttcctcgtgt gcgccaaaag cgcgggcggc gcgccgcagg agttcgcgtg cacgcacatg
      601 ctcaccgagc tgcgccatcg cgatgccgcg cagcgccagc gccggcggat cgtcgcgcag
      661 cgcgacgcgc ttcagcgcac cgagcggatc gccggcggcg agcgctcggg cggaggcggt
      721 gatcagggag tccatgaagg aaagactaca ccggcgcaac tgtacgtgcc ggcattgccg
      781 tgacagtcgc cgctcctccc agccctgtca caatctcagg gcctcatccg tctgacaggc
      841 atggacggcg catggggcgc tgtcctcgat gaactgaaag gactgccatg tctcatgccg
      901 cccggattgc cctccacaaa catgcccccg ccctgctcga cgcccagctc aagctgtcga
      961 atgcggcaaa gcaaagttcg ctcggcgagc gattggtcga gctgatcaac ctgcgtattt
     1021 cacagatcaa cggctgcggc ttctgcgtcg atatgcattg gcgcgacctg ctccgccaag
     1081 gcgtggatcc gcgtcatctg aatgccgtcg ccgtctggcg cgaggcgcca ttcttcagcg
     1141 agcgcgagcg cgcggccctg cgctgggcgg agctggtcaa cggccagccg catgtcgagg
     1201 caagcgatgc ggagttcgcg cgggtgcgcg agtttttctc ggaaacagag gtcgtcgagc
     1261 tcggctatgc tgtggcggcc atcaagggtt ggaacatgat gaaccggagc ctgcagaacc
     1321 cgatcccgga agtgccggca ccggggatgt aaggctcgcg tacggggaat ggcgatgaac
     1381 gaaacggaag ccggcggccg cgaagccgtg ttcgcaggac tgcggccacg gctgttttcc
     1441 gccgcctacc ggatgctcgg cacccgggcc gatgccgagg acgtggtaca ggatgcctgg
     1501 ctgcgctggc aggcggccga ccacgccgcc ttgcgctcgc ctgaagcctg gctggtgacg
     1561 gtgacgacac gcctggcact ggatcggctg cgcgagcgca aggcggagcg cgagagctat
     1621 acaggctggt ggctgcccga gccgctggtg gagcttgatg agaatacgcc cgaaacggcg
     1681 gccgagctgg ccagcgacgt ctcgtttgcc ctgctctgga cgctggaacg gctgggcgcg
     1741 gaagagcgcg cggccttcct gcttcgccag gcctttgatc aggattacgc cgatatcgcc
     1801 gccttgctgg gaaagagcga agcgagctgc cggcaactgg tacaccgcgc tcaggaaaag
     1861 ctgcgcgatt cccgcccacg ctttcagccc gcgcccgagg cgcatcgcgc acttctcgcc
     1921 gcgtttaccg aggcagcccg gcgcgccgac cgggaagcca tgaaggcgct gctgtcgcct
     1981 gacgtgtcct ttgtcagcga cggtggcggc aagatgccgt ccttccatcg cgtgctgcga
     2041 ggggccggac gcatcgccgg ccttttctgg tggctcgagc agcagcatcc ggggaaggtt
     2101 cagtaccggt tggcccggat caacggcgga ccgggcctgc ttcgctacgt cgacggccgg
     2161 atcgagtccg cgcagtcgtt ccacttcgag gatggccaga tcgcggcggt gttcgtcgtg
     2221 cgcaatccgg acaagctggc cggcgtgccg cagcggcttc ggtcatggta gtacgggata
     2281 aaggatgctc tgcgccttgg ccggaaacac cgtgaactgt ttccagtcat ctccaccgga
     2341 gcgggcggag ctcacatccc cgtcacgctc ccccgctacg ggccgggccg atgcgcggct
     2401 gaccttgagt gtgctgccgg cgaaatggca ttgacccgca caaacatcag gaacatgcga
     2461 gtccgggttc acggcatgag gtcccgaaat ccgcaaatcg cgcagcccgg agcgcgtaag
     2521 atgatgaact gtggcgctcg gcatgcgcga ggcgcttgtg ccgcaacgtg cccgtcctga
     2581 ccagcatatc gagcatgcct ctgcgcccgt cagcaggcac aggagccgtc gcatgcagga
     2641 accgttcaat cccatggccg agcagaatgc gcttggcatt ctctggctca atgaggccgg
     2701 gtgcattacc tacgccaacg acgctgtcgc gcatttgatc agcaccggta ccgacacgct
     2761 cgtgggcaag ccgatcaagg agttgatcgt cgaactgcac ccgtcagcat gggttgaact
     2821 cgccacgtcg atcagcccgg gcgaatcgag gctgctgcag gtaaccctcc acaaggccgg
     2881 tcatggcagc gccgcggcgg aggcccacgt gcttcgcctc agtgacaagg gcaaccgctc
     2941 ctttgccgtt ttcctgcagg gcattgccac gccggtgcgt accgaggagt tgtttcgcct
     3001 gcagcacgac gtcattgaag cgctcgcccg ggacgagccg ttggccaaac tgctcaacag
     3061 gctttgcctc cggatcgaag cgatcgcgag ggatctgctc tgctcggtca tcctggtgga
     3121 cgagcatgac cgcgtgcatg tcgtagccgc gcccagcata ccgaagggct attcgatcgc
     3181 gatcgacggg cagccgatcg gcccaaaggc ggggtcgtgc ggaacggcct tgtacttcaa
     3241 tcagccggtc gaagtcagcg acatcgcaca tgatccgctc tgggaaaact acaggcagct
     3301 cgtattgccc atcgggctgg tagcctgctg gtcacaaccg atcaccgcgc gcgacggccg
     3361 ggttctggga tcgttcgcaa tttactatcg caagccccgc gggccgtccg agtttcatcg
     3421 gcaactggtc gaggtgtgca cccatctgac cgccatcgcc atcgagcgca cccggatgga
     3481 cgagcgattg cacgatcttg ccttccatga cgtgctgacc aagctgccca accgcaagct
     3541 gctggccgac cggggagcga tcgcactggc gaatgccgcg cacgcacagc acccggtcgc
     3601 gcttctgcac attgatctgg accgcttcaa gcgtatcaat gatgcacatg gccacgccgt
     3661 cggcgaccgc ttcctccagg cgtatgccga ccaactgcaa gccgccgtgc gcaagggtga
     3721 caccgtgagc cgggtgggta acgatgaatt cgtcctggtc ctgccgcgct gtgacgggca
     3781 gcaagccgcc catctggcgc agcggacgct ggacatcctg gctgagcatg tcatcctcgg
     3841 gcaaaagcga ttcgtcgctt cggcgagtat tggcatcagc ctgtatccgg atgatgcgaa
     3901 agacttccaa accttgttgc gatacgccga gatggcagcc catcaagtca agaaaggcgg
     3961 gggcaacggc tatcgctttt tcagccgtga gctcaatgcg gcgaatcagg agctggccgc
     4021 gcttgaacag gcgttgcgcg gagcgctggc ggcgcgacgc ttgagcgtgc attaccagcc
     4081 caagatggac ctcaaccgcg aatcggtcta tggcatcgag gcgctggcac gctggaacga
     4141 cccgcagctc ggcatggttc ctccggcgcg ctttattccg gtcgccgagg catccggact
     4201 gattcatgaa ctcgggcgct gggtactcga cgaagcctgc cggcagctcg ccgaatggcg
     4261 ggctgaagag ctggcgatac cgtatgttga agtcaacgta tcagccaagc aattgggc
//
LOCUS       DATLGF010000333         2304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_73217,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000333 DATLGF010000000
VERSION     DATLGF010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73217"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..>2304
                     /locus_tag="VM406_10170"
     CDS             287..>2304
                     /locus_tag="VM406_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HVL76373.1"
                     /translation="MTDPAELIAHFSPRISLQTLARGDGLLGMRPSGSFGPRGGSVTV
                     AQIDWVYTADDGRRWEIAAPSTVLNRRPASSQFLEDEAGRRVLLRDLWAEADALDKVW
                     DLGLLPLPADTLQWRGGPPESLRGPLWTLVQEDHFSDFWADRLPELQEQGWAVIVRPG
                     FAHDSVPVEAWRLIVSPETGEVLGKEVAARLRGRAPVVSGLGHPQGDSWLLTLGIEID
                     GQMHDVVPLLADLLRRDARWLNAQSVAAIGDDEIVRLRAPGGRRIDTPAAPVKAIVCG
                     MLDLLTDPRRGDGPLRLYASDALRFDVMCRGLLETQRERAGPHGAWQMEGEAGLRRLA
                     ARLASAGVPPAVSQPEGMGITLRPYQLQGLAWLQYLREHDLAGILADDMGLGKTAQAL
                     AHLLVEKRSGRLDLPALVVLPTSLVFNWQAEARRIAPALRVLTLHGANRADEFARMAR
                     HDVVLTTYPLLWRDIEALAAQPFHLLILDEAQTVKNAAGRSAGAARRIKARHRLCLTG
                     TPLENNLGELWAQFDFLMPGFLGDARSFARTWRKPIEVDGETVRAQLLAQRVRPFILR
                     RRKEDVAAELPPRTEIVERVRLQGRQRDLYESVRVAADEQVRRVLQRKGFEGAQIMIL
                     DALLKLRQVCCDPYLLKDVRHDPGMERAKIDVLRDMLPAMVEEGRRIL"
BASE COUNT          368 a          771 c          801 g          364 t
ORIGIN      
        1 ccagcgaggc ccaatgcgag tgcgcagaat acgagaggca ggaggcgtgt cattcggcag
       61 gaacggtgaa gtcggcgtgt ccggaaggaa tccggctcgt gtcaaggtga aatattgccc
      121 gacttccact tccgacagaa gggccgatca cattctccag atcatttcga acgccatgac
      181 aggcgcatgc ggcgagtcga tgcgggcttc gacgccgcgg tggacaatac ggcctttcga
      241 ttaccgtgca ccacaaaccg cggcaacgcc gtcggtattc atgaccatga cggacccggc
      301 cgagctcatc gcgcacttta gcccgcgcat ctcgctgcag accctcgcgc gcggggacgg
      361 cttgctcggc atgcggccgt ccggctcgtt cggacctcgc ggcggcagcg tgacggtcgc
      421 gcagatcgac tgggtctata ccgcggacga cggcaggcgc tgggagatcg cggcgccgtc
      481 taccgtgctg aaccggcggc ctgcctcatc gcagttcctc gaagacgaag ccggccggcg
      541 cgtgctgctg cgcgacctgt gggccgaggc cgacgcgctg gacaaggtct gggacctcgg
      601 cctgcttccg ctgcctgccg acacgctgca atggcgcggc ggcccgccgg agagcttgcg
      661 cggcccgcta tggaccctgg tgcaggaaga ccatttcagc gacttctggg cagaccggct
      721 gcccgaactg caggagcagg gatgggcggt gatcgtgcgc ccgggctttg cgcatgacag
      781 cgtgccggtg gaagcctggc gcctgatcgt cagccccgaa accggcgaag tgctcggcaa
      841 ggaggtcgcc gcgcgcttga gaggccgcgc gcccgtggtc tccgggctcg gccatccgca
      901 aggtgattcg tggctgctga cgctgggcat cgagattgac gggcagatgc atgacgtcgt
      961 gcccttgctg gccgacctgc tgcggcgcga cgcgcgctgg ctcaacgccc aaagcgtcgc
     1021 cgccattggc gacgacgaga tcgtccgcct gcgcgcgccg ggcggcaggc gcatcgacac
     1081 cccggccgcg ccggtcaagg ccatcgtgtg cggcatgctg gatctgctga ccgatccgcg
     1141 gcgcggcgac ggcccgctgc gcctgtacgc ctccgatgcg ctgcgtttcg acgtgatgtg
     1201 ccgcggtctg ctggaaaccc agcgcgagcg cgcgggtccg cacggcgcct ggcagatgga
     1261 gggagaggcc gggctgcgcc ggctcgccgc gcgcctggcc tcggccggtg tcccaccagc
     1321 ggtttcacag cccgaaggta tgggcatcac cttgcggccg tatcagttgc agggcctcgc
     1381 atggctgcaa tacctgcgcg aacacgatct ggccggcatc ctcgccgacg atatgggact
     1441 gggcaagacg gcacaggcgc tcgcccatct gctggtcgag aagcggtccg gtcggctcga
     1501 tctgcccgcg ctggtggtct tgcccacctc cctggtcttc aactggcagg ccgaggcgcg
     1561 gcgcatcgca cccgcgttgc gcgtgctgac gctgcatggc gcgaaccgtg cggacgaatt
     1621 tgcgcgcatg gcgaggcacg atgtcgtgct gaccacgtat ccgctgctgt ggcgcgacat
     1681 cgaggcattg gccgcgcagc ccttccatct gctgatcctc gatgaagcgc agacggtaaa
     1741 gaatgcggcc ggccgcagcg ccggcgcggc acgccgcatc aaggcgcgcc accgcctgtg
     1801 cctgaccggc acgccactgg aaaacaacct cggtgagttg tgggcgcagt tcgacttcct
     1861 gatgcccggc ttcctcggcg acgcgcgcag ctttgcgcgc acttggcgca agccgatcga
     1921 agtcgacggc gaaaccgtgc gcgcgcaact gctcgcgcag cgcgtgcgcc cgttcatcct
     1981 gcgccgccgc aaggaagatg tcgccgccga gctgccgccg agaaccgaaa tcgtcgagcg
     2041 tgtgcggctg caaggccggc agcgcgatct gtatgagagc gtgcgcgtgg ccgccgacga
     2101 acaggtgcgc cgcgtcctgc agcgcaaggg cttcgaaggc gcgcagatca tgatcctcga
     2161 tgccctgctc aagctgcggc aggtatgctg cgatccctat ctgctcaagg acgtgcggca
     2221 cgaccccggc atggagcgcg cgaagatcga cgtgctgcgc gacatgctgc ccgccatggt
     2281 ggaggagggc cggcgcatcc taat
//
LOCUS       DATLGF010000334         3876 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_151330, whole genome shotgun sequence.
ACCESSION   DATLGF010000334 DATLGF010000000
VERSION     DATLGF010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151330"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VM406_10175"
     CDS             <1..255
                     /locus_tag="VM406_10175"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HVL76374.1"
                     /translation="EQALAAADAGKLAHASHRFLSSIENLGALRMRAPCVELERIANT
                     GHLEQAPPLLRMLQDEFAVAREHLRVMTAEGGYARGRRST"
     gene            376..1233
                     /locus_tag="VM406_10180"
     CDS             376..1233
                     /locus_tag="VM406_10180"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HVL76375.1"
                     /translation="MLLQAPPSSPLPTLFASIFGLLVGSFLNVVIHRVPRMMQRESDN
                     YVAHESGQPLPHTDRYNLMVPRSSCPHCGHQITALENIPVISYLVLGGKCASCKAPIS
                     ARYPAVELLTAVLSALMIWQFGSGVAGFAALVFTWLLIAMTFIDADTQLLPDDLTLPL
                     LWLGLLVNLNATFVPLHEAVIGAAAGYLVLWAVYWVFKLATGKEGMGYGDFKLLAALG
                     AWMGWKMLPLIILLSSLVGAVVGIALILFARHGRDKPIPFGPYLAAAGLIALLYGDTL
                     VAAYLGFLF"
     gene            1235..1852
                     /gene="coaE"
                     /locus_tag="VM406_10185"
     CDS             1235..1852
                     /gene="coaE"
                     /locus_tag="VM406_10185"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921461.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HVL76376.1"
                     /translation="MSGHARFSIGLTGGIGSGKTTVADMFAARGAAVIDTDVIAHTLT
                     APGGAAIDAIRREFGAPFLAPDGSMDRARMRQYVFEEPGARARLEHILHPLIRSETER
                     AAAEAHGAYLMFVVPLLVESGSWRQRVSRVLVVDCPEDVQLRRVMMRSGLSEAQVRAI
                     MAAQVARQERLAAADDVIDNSGESLALVPQVDRLHAAYLQMAQHA"
     gene            1983..2738
                     /gene="zapD"
                     /locus_tag="VM406_10190"
     CDS             1983..2738
                     /gene="zapD"
                     /locus_tag="VM406_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HVL76377.1"
                     /translation="MITYEYPFNERIRTLLRLEDLYEKFTFFLHQEHPQHHHVALSTI
                     FEMLEVAGRADLKSDLLQELERQKQTLLGFRANPNVLPERLDAVLAEIDRCSAALMAS
                     QGKTGQNVRENEWLMGIRGRTIIPGGACEFDLPSYFAWQHRPARQRFEDISGWFAPLL
                     PLFEAIHLVLRLLRESGRSSRIVAERGSYQQMLQGKVYQMLRLTLASNLAVIPEISAN
                     KYMLWVRFTLQDGDMKPKPYESDVPFELTLCNF"
     gene            2748..2927
                     /locus_tag="VM406_10195"
     CDS             2748..2927
                     /locus_tag="VM406_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006713182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HVL76378.1"
                     /translation="MPVVVSCPTCGNKVEWIEANKYRPFCSDRCKQIDLGAWAEGKYA
                     IPATEPPKDDEHGER"
     gene            complement(3023..>3876)
                     /locus_tag="VM406_10200"
     CDS             complement(3023..>3876)
                     /locus_tag="VM406_10200"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HVL76379.1"
                     /translation="VGETGTGKELVAQACHASSSRAGAPFLALNCAALPESLAESELF
                     GYVSGAFTGAQRGGKPGLIEMAEGGTVFLDEIGEMSLYLQAKLLRFLNDGKFRRIGSE
                     REIKVNVRIISATHRNLRKMVADGSFREDLFYRLNVLQLDVPPLRERPDDILLLARHF
                     IQRACAQAQKPLCRLTASACAALVANSWPGNVRQLQNVVFRAITMTDKRALDAADLDL
                     AEDSIAPASLSEAESEDWEKSVAAFERALLERLYPAHPSSRKLARRLNTSHSMIATKL
                     RKYGIPG"
BASE COUNT          648 a         1296 c         1223 g          709 t
ORIGIN      
        1 gaacaggcgc tggccgccgc agacgcgggc aagctcgcgc acgccagtca ccgcttcctg
       61 tcgagcatcg agaatctcgg cgcgctgagg atgcgtgcgc cctgcgtgga gctggaacgg
      121 atcgcaaaca ccggccatct cgaacaggcg cccccgctgc tgcgcatgct gcaggatgag
      181 tttgcagtcg ctcgcgaaca cttgcgcgtg atgaccgccg agggcggcta cgcgcgcggg
      241 cgccgcagca cctagtcaag gcggtttgcc tgcctgctgt ttgacggata tcatgcccga
      301 cggacaaggc gacgattgcc atcgccgctg tcgcccacat cccgatccag caaggtttcc
      361 cgaatgcttc acgacatgct tctgcaggcg cctccttcga gtcccctgcc cacgctattc
      421 gcctccatct tcggcctatt ggtcggcagc ttcctgaacg tggtgatcca tcgcgttccg
      481 cgcatgatgc agcgtgaaag cgacaactac gtcgcgcatg aaagcggcca accgctgccg
      541 cacaccgacc gctacaacct gatggtgccg cgctcttcct gcccccactg cggacaccag
      601 atcacggcgc tggagaacat cccggtgatc agctacctgg tcctcggcgg caaatgcgcg
      661 tcctgcaagg cgccgatctc ggcacgctac cctgcggtgg aactgctcac cgcagtgctg
      721 tcggccctga tgatctggca gttcggcagc ggcgtggccg gattcgccgc gctggtcttc
      781 acctggctgc tgatcgcgat gaccttcatc gacgccgaca cccagctgct gccggacgat
      841 ctcacgctgc cgctgctgtg gctcggcctg ctggtcaatc tgaacgcgac cttcgtgccg
      901 ctgcatgagg cggtcatcgg cgcggccgca ggctatctgg tgctatgggc ggtatattgg
      961 gtgttcaagc tggccaccgg caaggaaggc atgggctatg gcgacttcaa gctgctggcc
     1021 gcgctcggcg cctggatggg ctggaagatg ctgccgctga tcatcctgct gtcttcgctg
     1081 gtcggcgcgg ttgtcggcat cgcgctgatc ttgtttgcgc gccatgggcg cgacaagccg
     1141 attccgttcg gcccctacct cgccgccgcc ggcttgatcg cgctgctgta tggcgacacc
     1201 ctggtcgcag cctatctcgg cttcctgttc tgacatgagc ggccatgcgc gcttttccat
     1261 cggcctgacc ggcggcatag gcagcggcaa gaccacggtt gcagacatgt tcgccgcgcg
     1321 cggcgcggcc gtcatcgata ccgatgtgat cgcgcacacg ctgaccgccc cgggtggggc
     1381 cgcaatcgat gcgatccggc gcgagttcgg cgcgcccttt ctcgcgcccg acggttcgat
     1441 ggaccgcgcc cgcatgcgcc agtacgtgtt cgaggaaccg ggcgcgcgtg cccggctcga
     1501 gcacatcctg catccgctga tccgcagcga gaccgagcgc gcggcagcag aggcgcacgg
     1561 cgcctacctg atgttcgtgg tcccgctcct cgtcgagtcg ggcagctggc gacagcgggt
     1621 gtcccgcgtg ctcgtggtcg actgcccgga ggacgtgcag ctgcgccgcg tgatgatgcg
     1681 cagcggcttg tccgaggcgc aggtgcgcgc gatcatggcg gcacaggtcg cgcgccaaga
     1741 gcggctggcg gcggctgatg acgtcatcga caacagcggc gaatcgctcg cgctggtgcc
     1801 ccaggtcgat cgcctgcatg cggcgtatct acagatggcg cagcacgcct gatcttccgg
     1861 tccagcggcg cgcgagctgc aacaacccgt ttcctcgttt gtattttttt atcgtatggg
     1921 tcagaatcac ataattctga tcgtccacga cttgttcgcg aagtcaacga gggaccgcca
     1981 ttttgattac ctacgaatac cctttcaacg agcgtattcg cacactgctg cggcttgagg
     2041 acctgtacga gaagttcacc ttcttcttgc atcaggagca tccccagcac caccacgtcg
     2101 ccctctccac gatcttcgag atgctcgaag tggccgggcg cgccgacctg aagtccgatc
     2161 tgctgcagga actcgagcgc cagaagcaga cgctgctcgg ctttcgcgcc aatccgaatg
     2221 tcctccccga acggctcgac gcggtgctgg ccgagatcga ccgctgcagc gcagcattga
     2281 tggcgtccca aggcaagacc ggccagaacg tgcgcgagaa cgaatggctg atgggcatcc
     2341 gcggacgcac gatcatcccc ggcggcgcct gcgagttcga cctgccgtcg tacttcgcct
     2401 ggcagcaccg ccctgcccgt cagcgcttcg aggacatctc cggctggttc gcgccgctgc
     2461 tgccgctgtt cgaggccatc catctggtgt tgcgcctgct gcgcgaatcg ggccgctcga
     2521 gcagaatcgt ggccgagcgc ggcagctacc agcagatgtt gcagggcaag gtctaccaga
     2581 tgctgcgcct gacgctcgcg tcgaaccttg ccgtgattcc cgagatctca gcgaacaagt
     2641 acatgctgtg ggtgcgcttc acgctgcagg acggcgacat gaaaccgaag ccgtatgaaa
     2701 gcgacgtgcc gttcgagctg accctgtgca acttttaagt gattgcaatg ccagtcgtag
     2761 tctcctgccc gacctgcggc aacaaggtcg aatggatcga agccaacaag taccgcccgt
     2821 tctgctcgga tcgctgcaag cagatcgacc tcggcgcctg ggccgagggc aagtacgcga
     2881 tccccgcgac agaaccgccg aaggatgacg agcacggcga gcgctgaagc gccgtcgacc
     2941 atcgacatcc ctccgctgcg gcggcggaca agcggccctg gccgcatctt cccgctctga
     3001 ctgcgcgtcg aacgtcgcgc gtctatccag gtatcccgta tttgcgcagc ttggtcgcga
     3061 tcatcgagtg cgaggtgttc agccggcgcg cgagcttgcg gctggatgga tgggccggat
     3121 acagccgctc cagcagcgcg cgctcgaacg cagccaccga cttctcccag tcttccgact
     3181 ccgcctcgct caatgacgct ggagcaatgc tgtcttcggc cagatccagg tcggcggcgt
     3241 cgagcgcgcg cttgtccgtc atcgtgatcg cgcggaacac cacgttttgc aactgacgta
     3301 cattgcccgg ccagctattg gccaccagcg ccgcgcacgc cgacgcggtc aagcggcaca
     3361 agggtttctg cgcctgcgcg caggcgcgct ggatgaaatg gcgcgccagc agcaggatgt
     3421 cgtccgggcg ctcgcgcagc ggcggcacat ccagctgcag cacgttgagc cggtagaaaa
     3481 ggtcctcacg gaagctgccg tcggcgacca tcttgcgcag attgcgatgg gtcgcgctga
     3541 tgatgcgcac gttgaccttg atttcacgct cgctgccgat gcgccggaat ttgccgtcat
     3601 tcagaaagcg cagcagcttg gcctgcaggt agagcgacat ctcgccgatc tcgtcaagga
     3661 agacggtgcc accctcggcc atctcgatca ggcccggctt gccgccgcgc tgtgcgccgg
     3721 tgaaggcgcc cgacacgtag ccgaacagtt cgctctcggc caggctttcc ggcagcgccg
     3781 cgcaattgag cgcgaggaaa ggcgcgccgg cgcgggaact ggacgcatgg caggcctggg
     3841 cgaccagttc cttgccggtg ccggtctcgc cgacga
//
LOCUS       DATLGF010000335         7425 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_151335, whole genome shotgun sequence.
ACCESSION   DATLGF010000335 DATLGF010000000
VERSION     DATLGF010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 7425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7425
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151335"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VM406_10205"
     CDS             complement(<1..326)
                     /locus_tag="VM406_10205"
                     /inference="COORDINATES: protein motif:HMM:NF024402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HVL76380.1"
                     /translation="MTATSIEPAAGAAPAKAPPRIRDAFLHPSAWTMFFFGFASGLPF
                     LLVAGTLAYWLRENGITLRDITMIASAGMAYSFKFLWAPLVDRLRLPIIGRMGQRRSW
                     ILLMQLL"
     gene            complement(323..934)
                     /gene="metW"
                     /locus_tag="VM406_10210"
     CDS             complement(323..934)
                     /gene="metW"
                     /locus_tag="VM406_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HVL76381.1"
                     /translation="MKFDELSARRADLAFIAHWVAQGAQVLDLGCGDGTMLEYLQTEK
                     QCFGYGVEIDDDKIRACVERGVSVIQQDMRAGLALFADDAFDMVLCLSALQMMKDVEG
                     TLQGIARVGREAIVSFPNFAYWPHRLAILRGRMPVSKSLPYQWYDTPNVRYSTIKDFA
                     ELANKVGLEVLECIALHNGNPVSFLPNVRGSLAVFRLRKKTTP"
     gene            complement(931..2079)
                     /locus_tag="VM406_10215"
     CDS             complement(931..2079)
                     /locus_tag="VM406_10215"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006713310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HVL76382.1"
                     /translation="MPSVGIVSPRSMHFPEPLQLKSGASLAGYDLVYETYGTLNEDRS
                     NAVLICHALNASHHVAGMYEGVDGSKNTGWWDNMVGPGKPVDTDRFFVIGVNNLGSCF
                     GSTGPMHTNPATGKPYGAGFPVVTVEDWVNAQARLADALGIERFAAVMGGSLGGMQAL
                     SWSMLYPERLAHCVVIASTPKLSAQNIAFNDVARQAILTDPDFHGGDFYAHGVVPKRG
                     LRVARMVGHITYLSDDSMAEKFGRDLRNGGYQFSYGIDFEIESYLRYQGDKFSEYFDA
                     NTYLLITKALDYFDPAKDYDDDLTRALADTRAQFLLVSFTTDWRFSPERSREIVQALV
                     NNKRRVSYAEIDAPHGHDAFLLEDARYMNVVRAYYDQVWRDVQAGGAA"
     gene            complement(2519..4273)
                     /gene="ptsP"
                     /locus_tag="VM406_10220"
     CDS             complement(2519..4273)
                     /gene="ptsP"
                     /locus_tag="VM406_10220"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HVL76383.1"
                     /translation="MASFSLHGIPVSRGIAIGRAHLLAPAALDVRHYLVSEEQVEAEV
                     ARLQQAIATVHQELQTMWRDLPKDSPAELGAFIDVHVLILCDPLIAEVPLEIIRSRHY
                     NAEWALVTQIDELSAQFDEVEDLYLRERRLDIQQVGERVLKVLTGSASALPSNNGGDA
                     TAGQVIVVAHDISPADMLQFKDRAFGGFVTDLGGPNSHTAIVARSLDIPAAVGMFNAS
                     ALIRHDDMVIIDGDAGVVIVDPTPDVLEQYRERQHRQLRERKKLGKLKKTPAITRDGT
                     PITLMANIELPEDCAGALEQGATGVGLFRSEFLFMGRAGAVGKLPSEDEQFESYRKAV
                     VMMKGRPVTIRTLDIGADKPLDAAGQTAQNPALGLRAIRYCLAEPQMFLTQLRAILRA
                     SAYGSIKLMIPMLAHVGEIEQVLAMIEQAKAQLREAGRKFDPQMPVGAMIEVPAAALA
                     LPMFIKRMDFLSIGTNDLIQYTLAIDRADHEVAHLHDPLHPAILYLLATTIAAGAKSG
                     IPVSVCGEMAGDVRFTRLLLGMGLREFSMHPSQLLVVKQEILNSDLNEIVPAVRKLLR
                     HPEPAAVVAAVERLRSAG"
     gene            complement(4279..4548)
                     /locus_tag="VM406_10225"
     CDS             complement(4279..4548)
                     /locus_tag="VM406_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HVL76384.1"
                     /translation="MVQQEIEIVNKLGLHARASAKLTQLAARHASEVWISRNGRRVNA
                     KSIMGVMMLAAGKGAKVTLEADGADEEACIAALSELINNKFGEGE"
     gene            complement(4567..4974)
                     /locus_tag="VM406_10230"
     CDS             complement(4567..4974)
                     /locus_tag="VM406_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HVL76385.1"
                     /translation="MIGILLLTHAPLGSAFIDAATHVFRARPERLEAVDVRADQDPAE
                     VSRVALDVIRRIDDGTGVLVITDVMGGTPSNCTLKLCSPGHVEVIAGISLPMLLRALT
                     YRQHTLDVVVEMALAGGQGGAVRVDNRVRIGPN"
     gene            complement(4979..5938)
                     /gene="gshB"
                     /locus_tag="VM406_10235"
     CDS             complement(4979..5938)
                     /gene="gshB"
                     /locus_tag="VM406_10235"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HVL76386.1"
                     /translation="MKFAFLADPLDRFKIYKDTTYSMMAEAARRGHEVYAFQQKDMAF
                     KDGRLQADVARIHMTGDADRWYKADPAQTTGLEAFDCVLVRKDPPFDMEYVYLTYLLE
                     VAEKQGARVFNKPEAIRSHNEKLAIAQFPEYIAPTLVTSDIAHVRSFHAHHRDIILKP
                     LDGMGGIGIFRVKEDGLNLGSIVETLTDNGRRTIMVQRFIPEIVDGDKRILLIGGQVV
                     PYSLARIPQGSEVRGNLAVGGLGVAKALSERDREIGEGLAQRLLERGLLLVGLDVIGD
                     YMTELNVTSPTCFREITDQTGFDVPALFIDALERCCAEVPPSA"
     gene            complement(5941..7239)
                     /gene="gshA"
                     /locus_tag="VM406_10240"
     CDS             complement(5941..7239)
                     /gene="gshA"
                     /locus_tag="VM406_10240"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HVL76387.1"
                     /translation="MVPHLVTALNGPLLDLEKKILSATPAIERWFRLEWQEHTPPFYC
                     SVDLRNAGFKLAPVDTNLFPGGFNNLSPEMLPLAVQAAMAAIEKYCPDAKNLLLIPEK
                     HTRNTFYLQNVSRLIQIFRQTGLNVRLGSLSDEVKEPTPIDLPDGTTITLEPLERSAN
                     GRRLGLKNFDPCTILLNNDLSSGIPPILENLREQNLLPPLHAGWAVRRKTNHFAAYDE
                     VVKKFAKMVDVDPWMLNPYFAKCSEIDFHERTGEERLAAHVDTVLAKIRKKYKEYGIK
                     EQPFVIVKADAGTYGMGIMTVKDASEVKDLNRKQRNKMAVVKEGLQVSDVIVQEGVHT
                     FEHINEAVAEPVVYMIDRYVVGGFYRVHSERGVDENLNSPGAHYVPLAFAQQHALPDL
                     QAKPGTAPPNRFYMYGVVARMALLAASIELEKTDPNPEVY"
BASE COUNT         1306 a         2318 c         2422 g         1379 t
ORIGIN      
        1 agcaattgca tcagcaggat ccagctgcgc cgctgcccca tgcgcccgat gatgggcaag
       61 cgcagccggt cgaccagcgg cgcccacagg aatttgaacg aatacgccat gccggcgctg
      121 gcaatcatcg tgatgtcgcg cagcgtgatg ccgttttcgc gcagccagta cgccagcgtg
      181 ccggccacca gcaggaacgg caggccggag gcgaagccga agaagaacat cgtccaggcc
      241 gacggatgca ggaaggcgtc acggatccgt ggcggagcct tggccggtgc agcgccggcg
      301 gccggctcga tggacgttgc ggtcatggag ttgtcttttt acgaagtcgg aatacggcaa
      361 ggctgccgcg cacgttcgga aggaatgata cggggtttcc gttgtgcaag gcgatgcatt
      421 cgagcacttc gagcccgact ttgttggcaa gttccgcaaa atccttgatc gtggagtaac
      481 gcacgttcgg cgtgtcgtac cactggtacg gcagcgactt cgacaccggc atgcgtccgc
      541 gcaggatggc caggcggtgt ggccagtacg cgaagttggg gaaggagacg atcgcctcgc
      601 ggccgacgcg ggcgatgccc tgcagcgtgc cttccacatc cttcatcatc tgcagcgccg
      661 acaggcacag caccatgtca aatgcatcat cggcaaacag cgcaaggccg gcgcgcatgt
      721 cctgctggat caccgacacg ccgcgctcga cgcaggcgcg gatcttgtcg tcgtcgattt
      781 cgacgccata gccgaagcat tgcttctcgg tctgcaggta ctcgagcatg gtgccgtcgc
      841 cgcaacccag gtccagcacc tgcgcgccct gcgcgaccca gtgggcaatg aaggccaggt
      901 cggcacggcg cgcggacagt tcatcgaatt tcaagccgca cctcccgctt gcacgtcgcg
      961 ccagacctgg tcgtaatagg cgcgcaccac gttcatgtag cgcgcatctt ccaggaggaa
     1021 ggcatcgtgg ccgtgcggcg cgtcgatttc cgcgtatgac acgcgccgct tgttgttgac
     1081 cagcgcctgc acgatctcgc ggctgcgctc cggcgagaag cgccagtcgg tcgtgaatga
     1141 aacgagcagg aactgcgccc gggtgtcggc cagcgcgcga gtgagatcgt cgtcgtagtc
     1201 cttggcgggg tcgaagtagt cgagagcctt ggtgatcagc aggtaggtgt tggcgtcgaa
     1261 gtactcggag aacttgtcgc cctgatagcg caggtaggac tcgatctcga agtcgatgcc
     1321 gtagctgaac tgataaccgc cgttgcgcag gtcgcgtccg aatttctcgg ccatgctgtc
     1381 gtcggacagg taggtgatgt ggccgaccat gcgcgcgacg cgcagtccgc gcttgggcac
     1441 cacgccgtgc gcgtagaaat cgccgccgtg aaaatccgga tcggtcagga tggcctggcg
     1501 ggcgacgtcg ttgaaggcaa tgttctgcgc cgacagcttt ggcgtcgagg cgatcaccac
     1561 gcagtgcgca agccgctcgg ggtagagcat gctccaggac agcgcctgca tgccgccgag
     1621 cgagccaccc atcacggccg caaagcgttc gatgccgagc gcatcggcca ggcgcgcctg
     1681 cgcgttcacc cagtcctcca ccgtcacgac gggaaagcct gcgccgtaag gcttgccggt
     1741 ggcggggttg gtgtgcatcg gcccggtcga gccgaagcag gaaccgaggt tgttgacgcc
     1801 gatgacgaag aagcggtcgg tatcgaccgg cttgccgggg ccgaccatgt tgtcccacca
     1861 gccggtgttc ttgctgccgt ccacgccttc gtacataccg gcgacgtggt gggaggcgtt
     1921 cagcgcatgg cagatcagca ccgcgttcga acggtcttcg ttcagggtgc cgtaggtttc
     1981 atagacgagg tcgtagccgg cgagcgaggc gccgcttttc aattgcagcg gctcggggaa
     2041 atgcatcgat cgcggcgaga caattccaac ggaaggcata ggatccagac cagttttagt
     2101 ggcgtggcgc gatgaggcgg aaaacaaaaa agcccgaaag cgtttcagct tttcgggcta
     2161 gattgggctc ggtcttcaag cacgctttag ccgtatttgt taggcgcccg caagctgatt
     2221 atcaaatcgg cgcaccgcgt aagaatagcg gagcgggggg atggcgtcaa accaggagcg
     2281 gttggggcat cgccggtgct tgattcgatg cggcatgcct ggcttgtcat cgtgatttac
     2341 tccgcgcttg tcatcctgag ccgcggacga aggatctcgt cgccgtcatg atgcgtcgac
     2401 ccgtgaatcg gcggatgagg tccttcgctg gcgctcagga cgaaaaactt cacctgtcat
     2461 cctgagccgc aggcgaagga tctcatccgc ccaatccggc aaagtcggtg acgcgccctc
     2521 aaccagccga gcgcaagcgc tccaccgccg ccaccaccgc ggccggctcc ggatgccgca
     2581 gcagcttgcg taccgccggc acgatctcgt tcaaatcgct gttgaggatt tcctgcttga
     2641 cgaccagcag ctgcgacgga tgcatcgaga attcgcgtaa tcccatgccg agcagcagcc
     2701 gggtgaaacg cacgtcgccc gccatctccc cgcatacgga aacaggaatg ccggacttcg
     2761 cgccggccgc gatggtggtc gcaagcagat acagaatggc cggatgcaag gggtcgtgca
     2821 ggtgggcgac ttcatggtcg gcgcgatcga tggccagcgt gtactggatc aggtcgttgg
     2881 tgccgatcga caggaaatcc atgcgcttga tgaacatcgg cagcgccagc gcggcggccg
     2941 gcacttcgat catcgcaccg accggcatct gcggatcgaa cttgcggccg gcctcgcgca
     3001 gctgcgcctt ggcctgctcg atcatcgcca gcacctgctc gatctcgccg acatgcgcga
     3061 gcataggaat catcagcttg atcgaaccat aggcggacgc gcgcagaatg gcccggagct
     3121 gcgtcaggaa catctgcggc tcggccaggc agtagcggat cgcgcgcaga ccaagcgcag
     3181 ggttctgcgc ggtctggccg gcggcgtcga gcggcttgtc ggcgccgatg tcgagcgtgc
     3241 ggatggtcac cggacgtccc ttcatcatca ccacggcctt gcggtaggac tcgaactgct
     3301 cgtcctcgct cggcagcttg ccgaccgcgc cggcacggcc catgaacagg aattccgagc
     3361 ggaacagtcc gacaccggtt gccccctgct ccagcgcccc cgcacagtcc tcgggcagct
     3421 cgatgttcgc catcagcgtg atcggggtgc cgtcacgggt gatggccggc gtcttcttca
     3481 gcttgccgag cttcttgcgt tcgcgcagct ggcgatgctg gcgctcgcgg tactgctcga
     3541 gcacgtccgg cgtcgggtcg acgatcacca ccccggcgtc gccatcgatg atcaccatgt
     3601 cgtcgtggcg aatcagcgcg gaggcgttga acatgccgac cgcggcaggg atgtccagac
     3661 tgcgcgcgac gattgcggta tgcgagttcg gcccgcccag gtcagtgacg aagccgccga
     3721 atgcgcggtc cttgaactgc agcatgtccg ccggggaaat gtcgtgcgcg accacgatca
     3781 cctggccggc ggtcgcgtcg ccgccgttgt tcgaaggcag ggccgaggcc gagccggtga
     3841 gcactttcag cacgcgctcg ccgacctgct ggatgtcgag ccggcgttcg cgcaggtaga
     3901 gatcctcgac ttcatcgaac tgcgcgctga gctcgtcgat ctgcgtgacc agcgcccatt
     3961 cggcgttgta atgccggctg cggatgatct ccagcggcac ttcggcgatc agcggatcgc
     4021 acaggatcag cacgtgcacg tcgatgaagg cgccgagttc ggccggcgaa tccttcggca
     4081 ggtcgcgcca catcgtctgc agttcctggt gcacggtcgc gatcgcctgc tgcagccttg
     4141 cgacttcagc ttccacctgc tcctcggaca cgaggtagtg gcgcacatcg agcgcggccg
     4201 gcgcaagcag gtgcgcacgg ccgatcgcga tgccccgcga taccggaatg ccgtggagcg
     4261 aaaaggaagc catgagactc actcgccttc gccgaacttg ttgttgatca gctccgacag
     4321 cgcggcgatg caggcttctt catcagcacc gtccgcttcc agcgtgacct tcgcgccctt
     4381 gccggcggcc agcatcatca cgcccatgat ggatttcgcg ttgacgcgac ggccattgcg
     4441 cgagatccag acctcgctcg catgcctggc cgccagctgg gtgagcttgg ctgacgcgcg
     4501 ggcatgcaga ccgagcttgt tcacgatttc gatttcctgt tgaaccatgt cttgttgttc
     4561 ttgctgttaa ttcggtccga tgcgcacgcg gttgtcgacg cgtaccgcgc cgccctgtcc
     4621 gcctgcgagc gccatctcga ccaccacgtc gagcgtgtgc tggcgatagg tcagcgcgcg
     4681 cagcagcatc ggcaggctga tcccggcgat gacctccaca tggccggggc tgcacagctt
     4741 gagcgtgcag ttcgacggcg tgccgcccat cacgtcggtg atcaccagca cgccggtgcc
     4801 gtcatcgatg cgccggatga cgtcgagcgc gacgcggctc acctcggccg ggtcctggtc
     4861 ggcgcgcacg tccaccgcct cgagccgttc gggacgggcg cggaagacgt gcgtggcagc
     4921 gtcgatgaag gcgctgccca acggcgcgtg cgtgagaagg agaatgccga tcatgcaatc
     4981 aggccgaggg cggaacctcg gcgcagcaac gttcgagggc gtcgatgaac agtgcaggaa
     5041 cgtcgaagcc ggtctggtcc gtgatctcgc ggaagcaggt cgggctggtg acgttcagct
     5101 cggtcatgta atcgccgatg acgtccaatc ctaccagcag caggccgcgc tccagcagcc
     5161 gttgcgccag gccctcgcca atctcgcggt cgcgctcgga caaggccttg gccacgccga
     5221 ggccgcccac cgccaggttg ccgcgcacct cgctgccctg cggaatgcgt gccagcgaat
     5281 acggcaccac ctgaccaccg atcagcagga tgcgcttgtc gccgtccacg atctccggaa
     5341 tgaagcgctg gaccatgatg gtgcggcggc cgttgtcggt cagcgtctcg acgatggagc
     5401 ccaggttcag cccgtcttcc ttgacgcgga agatgccgat gccgcccatg ccgtccagcg
     5461 gcttgaggat gatgtcgcgg tggtgcgcat ggaagctgcg cacgtgggcg atgtcggagg
     5521 tcaccagcgt cggtgcgatg tattccggga actgggcgat cgccagtttt tcattgtggc
     5581 tgcggatcgc ctccggcttg ttgaacacgc gtgcgccctg cttctccgcc acttccagca
     5641 gataggtcag gtacacgtat tccatgtcga acggcgggtc cttgcgcacc agcacgcagt
     5701 cgaaggcttc cagcccggtg gtctgcgccg ggtcggcctt gtaccagcgg tccgcgtcac
     5761 ccgtcatgtg gatgcgcgcc acatccgcct gcagccggcc gtccttgaac gccatgtcct
     5821 tctgctggaa cgcgtacacc tcgtggccgc ggcgcgcggc ctccgccatc atcgaatagg
     5881 tggtgtcctt gtagatcttg aagcggtcga gcgggtcggc caggaatgcg aacttcatgc
     5941 tcagtagact tccgggttgg gatcggtctt ttccagttcg atcgacgctg ccagcagcgc
     6001 catgcgcgcg accacgccgt acatgtagaa ccggttcggc ggcgcggtgc ccggcttggc
     6061 ctgcagatcg ggcagcgcat gttgctgggc gaacgcgagc ggcacgtaat gcgcgccggg
     6121 cgagttcagg ttctcgtcca cgccgcgctc ggagtgcacg cggtagaagc cgccgacgac
     6181 atagcggtcg atcatgtaga cgaccggctc ggccaccgct tcattgatgt gctcgaaggt
     6241 gtgcacgcct tcctggacga tcacgtcgga gacctgcagg ccttccttga ccaccgccat
     6301 cttgttgcgc tgcttgcggt tcagatcctt gacctcgctc gcatccttga ccgtcatgat
     6361 gcccatgccg taggtgccgg cgtcggcctt gacgatcacg aacggctgct ccttgatccc
     6421 gtattccttg tacttcttgc ggattttcgc gagcaccgtg tcgacgtgcg cagcgaggcg
     6481 ctcctcgccc gtacgttcat ggaaatcgat ctcgctgcac ttggcgaaat agggattgag
     6541 catccagggg tcgacatcga ccatcttcgc gaatttcttc accacctcgt cataggcggc
     6601 gaagtggttg gtcttgcgcc gcaccgccca gccggcgtgc agcggcggca acaggttctg
     6661 ctcgcgcagg ttttcgagga tgggcggaat gccggaagac aggtcgttgt tgagcaggat
     6721 cgtgcaggga tcgaagttct tcaggccgag gcggcgcccg ttcgcactgc gctcgagcgg
     6781 ctccagcgtg atcgtggtgc catcaggcag atcgattggc gtcggctcct tcacctcatc
     6841 tgacagcgaa cccaggcgta cattcagccc ggtctggcgg aagatctgga tcaggcggct
     6901 gacgttctgc agatagaagg tattgcgcgt gtgtttttcc gggatcaaca gcaggttctt
     6961 ggcgtccggg caatatttct caatggccgc cattgccgcc tgaaccgcca gcggcagcat
     7021 ttccggcgac aggttgttga agccgcccgg gaacaggttg gtatcgacgg gcgcaagctt
     7081 gaacccggca ttgcgcagat ccaccgaaca atagaacggg ggggtatgct cctgccattc
     7141 gaggcggaac cagcgttcga tggccggcgt cgcggacaga atctttttct cgaggtcgag
     7201 cagggggccg ttaagggcgg tgacgaggtg gggaaccata ggaaaacttt cgcaggctgg
     7261 gcgttcttgc aatcaggctc ctcggattct aacggctaaa cccggggcgc gtatgccagc
     7321 attgtgtcgg aaacggctgg ccatcgtttc ccgccaagca acaatccacg atggcaccga
     7381 cgggggagaa cggccgcatc gtcgcggcca ggcgctccac tagtt
//
LOCUS       DATLGF010000336         2651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_92899,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000336 DATLGF010000000
VERSION     DATLGF010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92899"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VM406_10245"
     CDS             complement(<1..599)
                     /locus_tag="VM406_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVL76388.1"
                     /translation="MSIKTNAVRNVAQVLVCLAASTLALGVHAQQAQQWPAKPVKIIS
                     PYGAGGSNDTSGRLIAERLEAKYGQRMLVENKPGAGTRIANEFVARSDPDGYTLLWAA
                     APFAITAGAGIETRYDIRKDFIPVGPRVNGPVFLTVNADSPVKSVADFVKMARQKPEG
                     VTFASPGIGSGPHLTAELFGLRGGFKALNVHYRGDAAAYT"
     gene            complement(603..1919)
                     /locus_tag="VM406_10250"
     CDS             complement(603..1919)
                     /locus_tag="VM406_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HVL76389.1"
                     /translation="MDNSSMQALAREAVLYALPLYEMARMRAATCPRRDAQGRFAGST
                     PESTLRWVNHFIHTRQLLGPRHRQVVTPNNDTLYTNAWLDLSEGPVIVTAPASGARYY
                     VLGLLDMYTNPFGYIGTRTTGNDAGRFLLHGPNWNASVPAGVTPLACPTDAVWIIGRV
                     MVNDETDLAAAHAFQDGFSLTREDGGPAHRAYDVGMQPNEFMRDPQRFATVVNRALRE
                     NPPPAHETAMVERFAAVGIGAGQGDALSEAHTSLLGDAIGAVLEEVGAPQPSELGGGW
                     FLPVEVHESYGDDYFARAQVARNYIGALGIEEAMYIMADRDADGAPLDGSHAYELHFP
                     PGGLPQVDAFWSITMYDKADCMLVDNPLNRYSLGDRSPTMRNDPDGGLRLLFSAQPPK
                     DPAAQGNWLPAPSAPFYLTLRLYMPRRAHLERTFVYPSIQRLSSRG"
     gene            complement(1919..>2651)
                     /locus_tag="VM406_10255"
     CDS             complement(1919..>2651)
                     /locus_tag="VM406_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011518433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HVL76390.1"
                     /translation="HWTVSDEPAVAFWQNLFNAMRDFPPPAADAGIAALLRRAGIRIE
                     QGVDIASLKPAVVQGLVNAHAQAMQLIEAHTRSQFRRSWGYSLKLGKWGDDYLLRATT
                     AMKGLGALSADEAVYALGDYDANGELLDGRHRYELRFAPGMQPPAQAFWSVSLYGKDY
                     YFTPNPIGRYAVGNFTAGLRLEADGSLVIPISHEPPDDRSNWLPAPAGPFYLILRLYH
                     PSPDFIAGRYEIPALQRIDASRRAA"
BASE COUNT          426 a          869 c          927 g          429 t
ORIGIN      
        1 gtgtaggcgg cggcatcacc acggtaatgc acgttgagcg ccttgaagcc gccgcgcagg
       61 ccgaacagtt cggcggtcag gtgcgggccc gaaccgatgc cgggcgaggc gaaggtcacg
      121 ccctcgggtt tctgccgcgc catcttcacg aagtccgcga cggacttcac cggcgaatcc
      181 gcgttgacgg tcaggaatac cggaccgttc acgcgcgggc cgaccgggat gaaatccttg
      241 cggatgtcat agcgcgtctc gatgccggcc cccgcggtga tcgcgaacgg cgccgccgcc
      301 catagcagcg tgtagccgtc aggatcgctg cgcgcgacga actcgttggc gatgcgcgtg
      361 ccggcgcccg gcttgttttc caccagcatg cgctgtccgt acttcgcttc cagccgctcg
      421 gcgatcaggc gcccggaggt gtcattcgat ccgcccgcgc catacggcga gatgatcttc
      481 accggcttgg ccggccattg ctgcgcctgc tgcgcgtgca cacccaacgc cagggtggac
      541 gcggccaggc agaccaggac ttgtgcaacg ttgcgaactg cattcgtttt gatgctcatg
      601 agtcaccccc tagatgagag acgttggatg gacggataga cgaacgtgcg ctcgagatgc
      661 gcacggcgcg gcatgtaaag acgaagggtc aggtagaacg gtgcggacgg cgccggcagc
      721 cagttgccct gggctgccgg atccttaggc ggctgcgccg agaacagcag ccgcaggccg
      781 ccgtcggggt cattgcgcat cgttggcgag cggtcgccga gcgagtagcg gttcagcgga
      841 ttgtcgacca gcatgcagtc ggccttgtca tacatcgtga tcgaccagaa cgcgtcgacc
      901 tgcggcagcc cgccgggcgg gaaatgcagc tcgtacgcat gactgccgtc gagcggcgcg
      961 ccgtcggcat cacggtcggc catgatgtac atcgcttcct cgatgccgag cgcgccgatg
     1021 tagttgcgcg cgacctgggc gcgggcgaag taatcgtcgc cgtaggactc gtgcacctcg
     1081 acgggcagga accagccgcc gcccagctcc gagggctggg gtgcgcccac ttcctccagc
     1141 actgcgccga tcgcgtcgcc gagcagcgac gtatgcgcct ccgatagcgc atcgccctgt
     1201 cctgcgccga tgccgaccgc tgcaaagcgt tcgaccatcg ccgtctcgtg cgcgggcggc
     1261 gggttctcgc gcagcgcgcg gttgacgacg gtggcgaagc gttgcggatc gcgcatgaat
     1321 tcattgggct gcatgccgac gtcgtatgcg cgatgcgcgg gcccgccgtc ctcgcgcgtg
     1381 agcgagaagc cgtcctggaa cgcatgcgcg gcggcgagat cggtctcgtc gttaaccatc
     1441 acgcgaccaa tgatccagac cgcatcggtc gggcaggcaa gcggcgtgac gccggccggc
     1501 acgcttgcgt tccagttcgg accatgcagc aggaagcgcc ccgcatcgtt gccggtcgtg
     1561 cgcgtgccga tatagccgaa cggattggtg tacatgtcga gcaggccgag cacgtagtag
     1621 cgcgcgccgc tggcaggcgc cgtcacgatc accggcccct cggacaggtc gagccaggca
     1681 ttggtgtaca gcgtgtcgtt gttcggcgtg acgacctgcc ggtgccgcgg cccgagcagc
     1741 tggcgggtgt ggatgaaatg gttgacccag cgcagcgtcg attccggcgt gctgccggcg
     1801 aagcgcccct gcgcgtcgcg gcgcgggcag gtggcggcgc gcatgcgcgc catctcgtac
     1861 agcggcagcg catagagcac cgcctcgcgc gcgagcgcct gcatggaaga gttgtccatc
     1921 atgcggccct ccgtgacgca tcgatacgct gcagcgcggg gatctcatag cggccggcga
     1981 tgaaatccgg gctcgggtgg tacagccgca aaatcaggta gaacggtcct gcgggcgcgg
     2041 gcagccagtt gctgcgatcg tccggcggct cgtgcgaaat cggaatgacc agcgagccgt
     2101 ccgcctccag ccgcagcccg gcggtgaagt tgccgaccgc atagcggccg atcggattgg
     2161 gcgtgaagta gtagtccttg ccgtacagcg agaccgacca gaatgcctgc gccggcggct
     2221 gcatgccggg cgcaaagcgc agttcgtagc gatggcggcc gtcgagcagc tcgccgttgg
     2281 cgtcgtagtc gccgagcgca tacacggcct cgtcggcgga cagcgcgccc aggcccttca
     2341 tcgcggtggt ggcgcgcagc aggtagtcgt cgccccactt gccgagcttc aggctgtagc
     2401 cccagctgcg ccggaactgg ctgcgcgtat gcgcctcgat cagttgcatc gcctgcgcgt
     2461 gcgcattcac caggccctgg accacggcag gcttcagcga tgcgatgtcg acgccttgct
     2521 cgatccggat gccggcgcgg cgcagcagcg cggcaatgcc ggcatccgcg gccgggggcg
     2581 ggaagtcccg catcgcgttg aacaggttct gccagaacgc gaccgccggt tcgtcgctga
     2641 ccgtccagtg a
//
LOCUS       DATLGF010000337         2871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_213131, whole genome shotgun sequence.
ACCESSION   DATLGF010000337 DATLGF010000000
VERSION     DATLGF010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213131"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1914)
                     /locus_tag="VM406_10260"
     CDS             complement(<1..1914)
                     /locus_tag="VM406_10260"
                     /inference="COORDINATES: protein motif:HMM:NF025467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein
                     PgsC/CapC"
                     /protein_id="HVL76391.1"
                     /translation="MLSWFPLSIFPEGALSASVIVTVWVGITIVAITNLRLGWVLSGL
                     VIPGYMVPLLIVKPWAAGVVFAEGVITYALVWLYSEYATRYTGLSSFFGRDRFFALVL
                     ASVAVRIVMDGLLLPELGNWLLQNYDTAFDYRSNLHSFGLIIVALIANNFWKTGVVRG
                     AWPMFVQVGLTWLFVRYGLMEFTNFNMSSLAFMYEDSASSFLASPKSYIILLVTAFIA
                     SRLNLFYGWDFSGILIPSLLALQWYEPHKILTTFVEALVILVLADAVLRLPIFRTITM
                     EGARKLLLFFNVSFVYKFVLSWVLVLVAPELKVSDWFGFGYLLATLIALKIHDKGILA
                     RMTRATLQTSLAGVAAATVIGFVLVLMPDPEWNRSARAAGGVEIPSGPVATDARSLEQ
                     VLEDEKTRFYASSFHTRFATPLQHELDAFAAGVRRLLEYRQTGDPAALESARRWLSAA
                     NYEVLRVAEHYLVLRERTLDAQQRRHWGTYVLSLDAGSRLLVSVPAPLDEAGTYEAAL
                     TLFGRFGAQAFASSATSRYAGDDGAADVLNRPQTVFQVFHRELAAPDILQVRSRAAPA
                     SVLHVSGPVPGSLRLPELEQSTGSLSVEFSPSTDRNLQRRTLRGNFSEFWLNHDDAHR
                     MRPGGTGKPAPAME"
     gene            complement(1920..>2871)
                     /locus_tag="VM406_10265"
     CDS             complement(1920..>2871)
                     /locus_tag="VM406_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76392.1"
                     /translation="WAFGVERSALADRSTFMHAVDMLGRRLAEQLFEHRRGQMQPALA
                     DGWQHLLSDRRDFAIARMEENLHVIERHVHVLEDMFNGMEIPTALYGPFGRPIVANAG
                     MQALLRADESDGSAWSAADALAYLCAIPVQEARALLLRVVFETGRAERSARIGNDHCI
                     VQVSRLHDSGASLHTGQDVLKHMHGLMIQVSRQPVRREGEAAMAAGTVKAHESTEALA
                     ALSRSLPSAANEAGGNVAALDSDSASNDEAPALAAHEGDRNGEREVRRMPRAAKPSKA
                     DVLRRITELCKASARENARLALQSSAGAAYPFSIEQRAAG"
BASE COUNT          549 a          941 c          911 g          470 t
ORIGIN      
        1 ctccatggcc ggcgccggtt ttcccgttcc gcccggtcgc atgcgatggg catcgtcatg
       61 gttgagccag aactcggaga agttgccgcg caaggtacga cgttgcagat tgcgatcggt
      121 ggaaggagaa aactcgaccg acaggctgcc cgtcgactgt tccagttcgg gaagacgaag
      181 gctcccggga acggggcccg agacgtgcag cacggaggcc ggggcagccc gactgcgcac
      241 ctgcaggatg tcaggcgcgg ccagttcgcg atggaaaacc tggaacaccg tctgcggccg
      301 gttcagcaca tcggccgcgc cgtcgtcgcc ggcgtaacgc gaagtagctg acgaagcgaa
      361 ggcttgtgcc ccgaagcgcc cgaacagcgt cagcgccgct tcgtaggttc cggcttcatc
      421 caacggtgcc ggcacactca ccagcaggcg cgagcctgca tccaggctca acacataggt
      481 accccagtgg cggcgttgct gtgcgtccag cgtgcgctcg cgcaggacca ggtaatgttc
      541 cgcaacgcgc aggacttcat agttcgccgc cgacagccag cggcgcgcac tttccagcgc
      601 agccggatct ccggtctgcc gatactccag cagcctgcgc acgcctgccg caaacgcgtc
      661 gagttcgtgc tgcagcggtg tcgcaaaacg cgtatggaaa ctgctggcgt agaagcgagt
      721 tttttcgtct tccagcacct gttccaggct gcgtgcatcg gtggccaccg gcccggaagg
      781 tatctccacg ccgccggccg cgcgcgcgct gcgattccat tccgggtccg gcatcaggac
      841 cagcacgaaa ccgatgacgg tggcggccgc cacgccggcg agcgaggtct gcagggtcgc
      901 gcgcgtcatc cgtgccagga tgcccttgtc atggatcttc agtgcaatca gcgtggccag
      961 caggtaaccg aagccgaacc agtcgctgac cttcagttcc ggcgcgacca gcaccagcac
     1021 ccaggacagc acgaacttgt agacgaagct gacattgaag aacagcagca gcttgcgcgc
     1081 cccttccatc gtgatcgtgc ggaagatcgg caggcgcagc accgcatcgg ccagcacgag
     1141 gatgacgagg gcctcgacga aggtcgtcag gatcttgtgc ggctcatacc attgcagcgc
     1201 aagcagcgat ggaatcagga tgccgctgaa gtcccagccg tagaacaggt tcaggcgcga
     1261 ggcgatgaat gccgtcacca gcaggatgat gtaggacttc ggcgacgcca ggaaggagct
     1321 cgcgctgtct tcgtacatga aggccagcga actcatgttg aaattcgtga actccatcag
     1381 gccgtagcgc acgaacagcc aggtcaggcc gacctggacg aacatcggcc aggccccgcg
     1441 cacgacgccg gttttccaga agttgttcgc aatcagcgca acgatgatca ggccgaagct
     1501 gtgcaggttg ctgcgatagt cgaaggccgt gtcgtagttc tgcagcagcc agttccccag
     1561 ttccggcagc agcagcccgt ccatgacgat gcgaacggcc acgctggcga gaacaagcgc
     1621 aaagaagcgg tcgcgtccga agaaactcga caatccggtg tagcgggtcg cgtattccga
     1681 gtagagccat acgagagcgt aggtgatcac gccctcggca aacacgacac cggcagccca
     1741 gggcttcacg atcagcagcg gcaccatgta gccggggatg acgagacccg atagcaccca
     1801 gcccaggcgc aggttggtga tggccacgat ggtgatgccg acccagacgg tgacgatcac
     1861 cgaggccgac agcgcgcctt ccggaaatat cgacaaggga aaccagctca acatgactcc
     1921 tagccggctg cgcgttgctc gatcgagaac ggataagcgg cccccgcgct gctctgcagt
     1981 gcgaggcgcg cattctccct ggcgctcgcc ttgcaaagct cggtgatgcg ccttagcaca
     2041 tcggccttgc tcggcttggc cgctcggggc atccgacgca cttcgcgttc gccgttcctg
     2101 tcgccttcgt gcgcggcgag cgccggcgcc tcgtcgttgc tggcgctgtc gctgtcgagc
     2161 gcagccacat tcccgcccgc ctcattcgca gcgctgggca gtgatcggct cagggccgcc
     2221 agcgcttccg tggattcatg tgccttgacc gtccctgcgg ccatggccgc ttccccttcg
     2281 cggcgcacag gttgacggga aacctggatc atcaggccgt gcatgtgctt caggacgtcc
     2341 tgcccggtat gcaggctcgc gccgctgtcg tgcaggcgcg aaacctgcac gatgcaatga
     2401 tcgttgccga tccgcgccga acgctcggcg cggccggtct cgaacaccac gcgcagcagt
     2461 agcgcgcgcg cctcctgcac gggaatcgcg cacaggtagg caagcgcgtc ggcggccgac
     2521 catgcgctgc cgtcgctttc gtccgcgcgc agcaatgcct gcatgccggc gttcgcgaca
     2581 atcggacgcc cgaacgggcc gtacaaggcg gtcgggatct ccatcccgtt gaacatgtct
     2641 tcgagcacat gaacatgccg ttcgatgacg tgcaggtttt cctccatgcg cgcaattgca
     2701 aagtcgcgcc ggtcgctcag caggtgctgc cagccatcgg caagagccgg ctgcatctgc
     2761 ccacggcgat gttcgaacag ctgctcggcc aggcggcggc ccagcatatc gaccgcatgc
     2821 atgaaggtcg agcgatcggc cagcgcgctg cgctcaacgc cgaacgccca g
//
LOCUS       DATLGF010000338         5187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_92935,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000338 DATLGF010000000
VERSION     DATLGF010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5187
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92935"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VM406_10270"
     CDS             complement(<1..875)
                     /locus_tag="VM406_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVL76393.1"
                     /translation="MAAIKQSLPSRRFDAVIVGAGGSGMRASLQLAEAGLNVAVLSKV
                     FPTRSHTVAAQGGIGASLGNMSEDNWYWHMFDTVKGSDYLGDQDAIEFMCREAPKVVY
                     ELEHFGMPFDRNADGTIYQRPFGGHTANFGEKPVQRACAAADRTGHALLHTLYQRNVR
                     AKTHFFVEWMAIDVVRDADGDVLGVVALEMETGDVMILEAKTTLFATGGAGRIFAAST
                     NAFINTGDGMGMAARAGLPLQDMEFWQFHPTGVSGAGVLITEGVRGEGGILINANGER
                     FMERYDPNLKDLATR"
     gene            complement(878..1243)
                     /gene="sdhD"
                     /locus_tag="VM406_10275"
     CDS             complement(878..1243)
                     /gene="sdhD"
                     /locus_tag="VM406_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HVL76394.1"
                     /translation="MAQENIGPKRLVVGAHYGLRDWLAQRVSAIVMAVYTVVLLVLFL
                     AAGEFSYEGWAGLFANQWFKLLTFVTFLSLLYHAWVGMRNVWMDYIKPMALRLVLQSL
                     TVLWLIGCGAYAAQILWRV"
     gene            complement(1246..1653)
                     /gene="sdhC"
                     /locus_tag="VM406_10280"
     CDS             complement(1246..1653)
                     /gene="sdhC"
                     /locus_tag="VM406_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HVL76395.1"
                     /translation="MSEAAKNRPKYGVMRIVDVMHYRLPLPAYVSIFHRVSGVLLFLA
                     LPFILYLLEQSLTSELSFEYFREFVAQPFVKLIILVLVWGYLHHFFAGFRHMQLDFHI
                     GLDLPTARKTSAAVFIVSLLLTALVGLKLFGAF"
     gene            complement(1785..2576)
                     /locus_tag="VM406_10285"
     CDS             complement(1785..2576)
                     /locus_tag="VM406_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HVL76396.1"
                     /translation="MTTNPTLQSLNGNGAAAGGTAAGGASPTFSPLYQQIKALITQSL
                     QSGEWKPGELIPSEVELASRFKVSQGTVRKAIDELASENLVVRRQGKGTFVATHHEAR
                     AQYRFLRLMPDTGEPHSAENRILEVKRLRAPAEIARMLDLRAGDSVIFIRRVQSLEGA
                     PTIVEELWLPGALFRGLTAERLAEYKGPMYGLFETEFGTRMIRATEKIRAVAADTATA
                     ELLQVAAHTPLLSVERVSFTYGDKPVEVRRAYYLTERHHYRNELS"
     gene            2878..3867
                     /locus_tag="VM406_10290"
     CDS             2878..3867
                     /locus_tag="VM406_10290"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HVL76397.1"
                     /translation="MAKAPMRVAVTGAAGQIGYSLLFRIANGDMLGKDQPVILQLLEI
                     PDEKAQKALKGVMMELDDCAFPLLAGMSAHSDPMTAFKDADVALLVGARPRGPGMERK
                     DLLEANAQIFTVQGKALDAVASRNVKVLVVGNPANTNAYIAMKSAPNLPAKNFTAMLR
                     LDHNRALSQLAAKVGKPVTAIEKLCVWGNHSPTMYADFTHATVDGQPAKALVNDEAWT
                     KDVYLPTVGKRGAAIIEARGLSSAASAANAAIDHVRDWVLGTNGKWTTMGIPSDGSYG
                     IPEGIVFGFPVTTENGEYKIVQGLQIDAFSQERINLTLKELQEERDGVKHLVG"
     gene            3932..4903
                     /locus_tag="VM406_10295"
     CDS             3932..4903
                     /locus_tag="VM406_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HVL76398.1"
                     /translation="MHPSEVLFQDARRPVSIPVCDHYAGSEKLMRKSIALQQELGPVF
                     DITLDCEDGAAAGNEAAHAQLVGALVAGEDNRFGRLGARVHDLSHPGFEQDVALICEQ
                     AAKRLAYLVVPKVDGVGQVKQALAVIERHASRAGRNDLPVHVLIETHGALADARAIAA
                     LPQVECLSFGIMDFVSAHYGAIPGDAMRSPRQFTHPLVVRAKLEIAAACHAFGKVPSH
                     NVTTEISDTCVVAADAAQAAGELGYTRMWSIHPGQIGPILAALSPRTAEVDEAAGILG
                     AARDAAWGPIQFNGRLHDRASYRYYWTILQRAKAAGMPLPESAGSLL"
     gene            4960..>5187
                     /locus_tag="VM406_10300"
     CDS             4960..>5187
                     /locus_tag="VM406_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76399.1"
                     /translation="MKTVFSFAILVTALAGGPVLASSPAPAQQGQAQKKPAAASNAAK
                     PQARKEAPARKAAAPEPDVAGMAAEPYDCDLG"
BASE COUNT          994 a         1681 c         1645 g          867 t
ORIGIN      
        1 tcgcgggtgg cgaggtcctt gaggttcggg tcgtagcgct ccatgaagcg ctcgccgttg
       61 gcgttgatca ggatgccgcc ctcgccgcgc acgccttcgg taatcagcac gccggcgccg
      121 gatacgccgg tcgggtggaa ctgccagaac tccatgtcct gcagcggcag tccggcgcgt
      181 gcggccatgc ccatgccgtc gccggtgttg atgaaggcgt tggtggaggc ggcgaagatg
      241 cgccctgccc cgcctgttgc gaacaaggtg gtcttggctt cgaggatcat cacgtcgccg
      301 gtttccattt ccagcgcgac tacgccgagc acgtcgccgt cggcgtcacg caccacgtcg
      361 atcgccatcc actcgacgaa gaaatgggtc ttggcgcgca cgttgcgctg gtacagcgtg
      421 tgcagcagcg cgtggccggt acgatcggca gcggcgcagg cgcgctgcac cggcttttcg
      481 ccaaagttcg cggtgtggcc gccgaacggg cgctgataga tggtgccgtc ggcgttgcgg
      541 tcgaacggca tcccaaagtg ctcgagctcg tagacgacct tgggcgcctc gcggcacatg
      601 aactcgatcg cgtcctggtc gccgaggtag tccgaaccct tgacggtatc gaacatgtgc
      661 cagtaccagt tgtcctcgga catgttgccg agcgaggcgc cgatgccgcc ctgggcagcg
      721 acggtatgcg aacgggtcgg gaacaccttg gacagaacgg cgacgttcag gccggcttcg
      781 gccagttgca gcgaagcgcg catgccggag ccgccggcgc cgacgatcac cgcatcgaag
      841 cggcgggaag gaagggattg cttgattgct gccacgatta cactctccag agaatctgcg
      901 ccgcataggc gccacacccg atcagccaca gcacggtgag ggactgcagc acgagacgca
      961 gcgccatcgg cttgatgtag tccatccaga cgttgcgcat gccaacccag gcgtgataca
     1021 gcagcgagag gaaggtgacg aaggtaagca gtttgaacca ctggttcgcg aacaggccgg
     1081 cccagccctc atagctgaat tcgccggcgg ccaggaacag caccagcagc acgaccgtgt
     1141 agacggccat cacgatggcc gagacgcgtt gcgcgagcca gtcgcgcagg ccgtaatggg
     1201 cgccgacgac gaggcgcttc ggtccgatgt tttcttgtgc cattctcaga acgctccgaa
     1261 cagtttcagg ccgaccagcg cggtcagcag caggctgacg atgaacaccg cagcggaggt
     1321 cttgcgcgcg gtgggcaggt ccaggccgat atggaagtcg agctgcatgt ggcgaaagcc
     1381 agcaaagaag tggtgcagat agccccacac gagtaccagg atgatcagct tcacgaacgg
     1441 ctgcgcgacg aactcccgga agtactcgaa cgagagctcg gaggtgaggc tctgctccag
     1501 cagatacagg atgaacggca gggccaggaa cagcagcaca ccgctgaccc ggtgaaaaat
     1561 ggatacgtat gcaggcaatg gcagccgata atgcatcaca tcgactatgc gcatcacccc
     1621 gtacttcggc cggtttttcg cggcttcaga catgacagga tctccttggc ttaaaaatcc
     1681 cgtaattttc gctgattttt gtgcagcaca ccaactgata tcttttttcg cggcaacata
     1741 ctgctcgtcg cccggcaaaa ccggtggctt caccgccgtt acgctcaact cagctcatta
     1801 cggtagtgat ggcgctccgt caggtagtag gcacgacgca cctctaccgg cttgtctccg
     1861 taggtgaacg aaacgcgctc cacactcaag agcggcgtgt gcgcggcgac ctgcagcagc
     1921 tcggcggtcg cggtgtcggc cgccaccgcg cggatcttct cggttgcgcg gatcatgcgc
     1981 gtaccgaact ctgtctcgaa caagccgtac atcggcccct tgtattcggc caaccgctcc
     2041 gcggtcagac cgcggaacag cgcgccaggc agccacagct cctcgacgat ggtgggcgcg
     2101 ccttcgagcg actgcacgcg gcgaataaag atgacggagt ccccggcgcg cagatcgagc
     2161 atgcgggcga tttcggcggg ggcgcgcagg cgcttgacct cgaggatgcg gttttccgcg
     2221 ctgtgcggct caccggtgtc cggcatcagg cgcaaaaagc ggtactgcgc ccgcgcctca
     2281 tggtgcgtgg cgacgaaggt acccttgcct tgccggcgca cgacgaggtt ttccgaggcc
     2341 agttcgtcga tcgccttgcg caccgtaccc tggctgacct tgaagcggct ggccagctcg
     2401 acttcgctcg gaatcagctc gcccggtttc cattcgcccg actgcaggct ctgcgtaatc
     2461 agcgccttga tctgctggta caagggactg aaggtcggcg atgccccgcc cgccgcggtg
     2521 ccgccggccg cagcgccgtt gccattcagg gactgcaggg tgggattggt cgtcataagc
     2581 cgggatttca ccataaacac cggttaacgt ctagaaaaac cggccagtga tatgtcttat
     2641 ataagatata agataagcat tgacagaccg tatagccaag cctacactcg cgggcgaagt
     2701 cacgttgcac aaaacaaaca ttccgcgggc ggccgcatgc gcgggcgcgg catcgccttg
     2761 cgcgtggtct gtgagcgcga cgcgttcaac ggtattatta caggcttacc gaaaatcccg
     2821 acccatcgtc ctccgacgcc gtccaattcg ctttctttcc taccttggag attcaatatg
     2881 gctaaagccc ccatgcgcgt tgccgtcacc ggcgccgccg gtcaaatcgg ctattccctg
     2941 ctgttccgca tcgccaacgg cgacatgctc ggcaaagacc agccggtcat cctgcagctg
     3001 ctcgagatcc ccgacgagaa ggcgcagaag gcgctcaagg gcgtgatgat ggaactcgac
     3061 gactgcgcat tcccgctgct ggccggcatg agcgcgcaca gcgatccgat gacggcgttc
     3121 aaggacgcag acgtcgccct gttggtcggc gcccgtccgc gcggccccgg catggagcgc
     3181 aaggatctgc tcgaagccaa cgcccagatc ttcaccgtac agggcaaggc gctcgacgcc
     3241 gtcgcttcgc gcaacgtgaa ggtgctggtg gttggcaacc cggccaacac caatgcctac
     3301 atcgccatga agtcggcgcc gaacctgccg gccaagaact tcaccgcgat gctgcgcctg
     3361 gaccacaacc gcgcgctgtc gcaactggct gccaaggtcg gcaagccggt caccgcgatc
     3421 gagaagctgt gcgtgtgggg caaccactcg ccgacgatgt acgccgactt cacccacgcc
     3481 accgtcgatg gccagccggc caaggcgctg gtgaatgacg aagcctggac caaggacgtg
     3541 tacctgccca ccgtcggcaa gcgcggcgcc gcgatcatcg aggcgcgcgg cctgtcctcg
     3601 gcggcgtcgg cagccaacgc ggcgatcgat cacgtacgtg actgggtgct cggcaccaac
     3661 ggcaagtgga ccacgatggg catcccgtcc gacggctcct acggtattcc ggaaggcatc
     3721 gtattcggct tcccggtcac aaccgagaac ggcgaataca agatcgtcca gggcctgcag
     3781 atcgacgcgt tctcccagga gcgcatcaac ctgacgctca aggagctgca ggaagagcgc
     3841 gacggcgtca agcatctggt cggctaagac atcgacaagc gggctgcccg gcgaggcagc
     3901 ccgctttgca ttgactttca cagacccttt tatgcaccct tccgaggtct tgttccagga
     3961 cgcgcggcga ccggtctcga taccggtctg cgaccactac gccggctctg aaaagctgat
     4021 gcgaaagtcg atcgccctgc agcaagaact tggtcccgtg ttcgacatca ccctcgattg
     4081 cgaggatggc gccgcagccg gcaacgaggc ggcgcacgcg cagctggtcg gcgcgctcgt
     4141 ggcgggcgag gacaaccgct tcggccgcct gggcgcgcgc gtgcacgacc tgtcccatcc
     4201 cggtttcgag caggatgtcg ccctcatctg cgagcaggcg gcaaaacggc ttgcctatct
     4261 cgtcgtgccc aaggtagacg gcgtcgggca ggtgaagcag gcgctggccg tcatcgaacg
     4321 ccatgcgagc cgtgccggcc gcaacgatct gccggtgcat gtgctgatcg agacgcacgg
     4381 ggcgctggca gatgcccgtg cgatcgccgc gctgcctcag gtcgaatgcc tgtcgtttgg
     4441 catcatggat ttcgtctcgg cccactacgg cgcgattccc ggcgacgcga tgcgctcgcc
     4501 gcgccagttc acccacccgc tggtggtgcg cgcgaagctc gaaatcgccg ccgcatgcca
     4561 tgcgttcggc aaggtgccgt cccataacgt cacgaccgag atcagcgaca cctgcgtggt
     4621 cgcggccgac gccgcccagg cagccggcga actcggctac acgcgcatgt ggagcatcca
     4681 cccggggcag atcgggccga tcctggccgc gctgtcgccg cgcaccgcgg aagtggacga
     4741 agccgccggc atactcggcg cagcccgcga cgccgcctgg gggccgatac aattcaacgg
     4801 caggctgcac gaccgcgcca gctatcgcta ctactggacg atactgcagc gtgcgaaggc
     4861 ggccggcatg ccgttaccgg aatcagcagg gagtttgttg taagccatgc gcggcccata
     4921 cccataacac accagccgcg catatccaag gaagcatgaa tgaaaaccgt attctccttt
     4981 gccattctgg tgaccgccct tgccggcggc cctgtgctgg cgtcatcccc ggcgccggcg
     5041 cagcaaggcc aggcgcagaa gaagccggcc gccgccagca acgcggcaaa gccgcaggca
     5101 aggaaggaag cgccggcgcg caaggcggcc gctcccgagc cggacgtggc cggcatggcg
     5161 gcggagccat atgactgcga tctcggc
//
LOCUS       DATLGF010000339         2771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_46524,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000339 DATLGF010000000
VERSION     DATLGF010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46524"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2398)
                     /locus_tag="VM406_10305"
     CDS             complement(<1..2398)
                     /locus_tag="VM406_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVL76400.1"
                     /translation="MFNAIIHSSLRQRLIVLGVALLLMVYGALTLKRMPVDVFPDLNK
                     PTVTLMTEAGGLAPEEVEQLVTFPIETAMNGMPGVTRVRSVSGVGLSVVYVEFDWGTD
                     IYRNRQQVAERLALVNEQMPEGVTPQMGPVSSIMGEIMLIGLPIDTTQTTPMRVREYA
                     DWVLRPRLLTIPGVAQVIPIGGEVRQYRVIPNSAKMRDLGVGLDELEGALANFGSNTG
                     GGFSEIASKEFLIRNVARTTSLEHLQNLVVGNRNGQPIYLKQVADAGMAAGLKRGDGS
                     VNGAPAVILSVQKQPTADTVKLTAEVEAALGSLATSLPQGMKAPEILFKQADFIEKAV
                     SNVEEALRDGAIMVAVILFLFLMNVRTTFISLTAIPLSILVSVLVFKAFGLSINTMTL
                     GGIAIAIGELVDDAVVDVENVLRRLRQNKNAASPRAVLDVIASASIEVRSGIVYATMI
                     VVLVFVPLFALPGIEGRLFAPLGVAYIVSILASMLVSMTVTPVLCYYLLPKMKLLGHG
                     DSKLVTKLKAWDARLLHWSFDRSKTLLTGAAVAILLAAAMVPFFARSFLPAFNEGTLT
                     INVLLNPGSSLSESNRIGTLAEKLIAQVPEVVKVGRRTGRAELDEHAEGVHYSEVDVD
                     LRHSERNREEIMYDIRARLAPLPANISIGQPISHRLDHLLSGVRAQIALKIYGDDLDT
                     LRGLADDMRARLQAIPGLTDLQIEKQVLIPQLQVRVDYDKAASYGVNPADVTSVLERM
                     VGGDKVSQVIEGSRRFDVVLRLADEDRLQHGMANLLIETPNGRVPLGLIAEVIEADGP
                     N"
     gene            complement(2409..>2771)
                     /locus_tag="VM406_10310"
     CDS             complement(2409..>2771)
                     /locus_tag="VM406_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVL76401.1"
                     /translation="TESGEPLKLNLLGTGLELREQALPVQLRIVQPAPALAVGQPVKV
                     FVQLKEKKRGVVIPSTAAARAPSGDLVVWLHTAAERFVPHKVQVQPLDAASVVVTQGL
                     AGGERVVVQGVPALAQVR"
BASE COUNT          471 a          917 c          909 g          474 t
ORIGIN      
        1 ggttcggacc gtcggcctcg atcacttcgg cgatcaggcc cagcggcacg cggccgttcg
       61 gcgtttcgat cagcaggttc gccatgccgt gctgcaagcg gtcttcgtcg gccagccgca
      121 gtaccacgtc gaagcggcgg ctgccctcga tcacctgcga caccttgtcg ccgccgacca
      181 tgcgttcgag gacgctggtg acgtcggccg ggttgacgcc gtagctggcc gccttgtcgt
      241 aatcgacgcg cacctgcagc tgtggaatca gcacctgctt ttcgatctgg agatcggtca
      301 ggccggggat ggcctgcaga cgcgcgcgca tgtcgtcggc caggccacgc aaggtgtcga
      361 ggtcgtcgcc gtagatcttc agtgcgatct gggcgcgcac cccggacagc aggtgatcga
      421 gccggtgcga aatcggctgg ccgatgctga tgttcgccgg caacggcgca aggcgggcgc
      481 ggatgtcgta catgatctct tcgcggttgc gctcggaatg ccgcagatcg acgtcgacct
      541 cgctgtagtg cacgccctcg gcgtgttcgt ccagctcggc gcggccggtg cggcgcccca
      601 ccttcaccac ttcgggcacc tgggcgatca gcttctccgc cagcgtgccg atgcggttcg
      661 actccgagag gctggaaccc ggattgagaa gcacgttgat cgtcagcgtg ccttcgttga
      721 aggccggcag gaaggagcgg gcgaagaacg gcaccatggc cgcagccagc aggatggcaa
      781 cggctgcgcc ggtgagcagg gtcttcgagc gatcgaagga ccagtgcaac aggcgcgcat
      841 cccaggcttt gagcttggtc accagcttgc tgtcgccgtg gccgagcagc ttcattttcg
      901 gcagcaggta gtagcacagc accggcgtga ccgtcatcga caccagcatg ctggcgagga
      961 tggaaacgat gtaggcgacg cccagcggcg cgaacaggcg accttcgatg ccgggcagcg
     1021 caaacagcgg cacgaacacc agcaccacga tcatcgtggc gtacacgatg ccggaacgca
     1081 cttcgatcga ggccgatgcg atcacgtcca gaaccgcgcg cggcgaagcg gcattcttgt
     1141 tctggcgcag ccggcgcagc acgttctcca cgtccaccac ggcgtcatcc accagttcgc
     1201 cgatcgcgat cgcaatcccg cccagcgtca tcgtgttgat cgacagaccg aatgccttga
     1261 acaccagcac gctcaccagg atggaaagcg gaatcgcggt cagcgagatg aaggtggtgc
     1321 gcacgttcat caggaacagg aacaggatca ccgcgaccat gatcgcgccg tcgcgcaacg
     1381 cttcctcgac gttgctgacc gctttctcga tgaagtcggc ctgcttgaag agaatttcgg
     1441 gcgccttcat gccctgcggc aggctcgtgg cgagcgaacc gagagccgcc tcgacttcgg
     1501 cggtcagctt gacggtgtcg gcggtcggct gcttctgcac cgacaggatc acggcaggcg
     1561 cgccgttcac gctgccgtcg ccgcgcttga ggcccgccgc cattccggcg tcggccacct
     1621 gcttcagata gatcggctgg ccgttgcgat tgccgaccac caggttctgc aggtgctcca
     1681 ggctggtcgt gcgcgcgacg ttgcggatca ggaattcctt cgaagcgatt tccgaaaacc
     1741 cgccgccggt attgctgccg aagttcgcga gcgcgccttc cagttcgtcc agaccgacgc
     1801 cgaggtcgcg catcttcgcg ctgttcggaa tcacgcgata ctggcgcact tcgccgccga
     1861 tcggaatcac ctgggccaca ccgggaatcg tcagcaggcg cggacgcagc acccagtcgg
     1921 cgtactcgcg cacgcgcatc ggggtggtct gggtcgtgtc gatcggcagg ccgatcagca
     1981 tgatctcgcc catgatggag ctcaccggtc ccatctgtgg tgtcacgcct tccggcatct
     2041 gctcgttgac cagcgcgagg cgctcggcca cctgctggcg gttgcgatag atgtcggtgc
     2101 cccagtcgaa ctcgacgtag accacggaca ggccgacgcc cgataccgaa cgcacgcgcg
     2161 tgacgcccgg catgccgttc atcgcggtct cgatcgggaa ggtgacgagc tgctcgacct
     2221 cctccggtgc gaggccgccg gcttcggtca tcagcgtgac ggtcggcttg ttcaggtcgg
     2281 gaaagacgtc tacgggcatg cgcttcaagg tgagcgcacc gtagaccatc agcagcaggg
     2341 ccacgccgag cacgatcagg cgctggcgca ggctggaatg aatgatggcg ttgaacacgt
     2401 cggctctcct agcggacctg cgccagggcc ggcacgccct gcaccaccac gcgttcgccg
     2461 cccgccaggc cctgggtcac gacgaccgat gcggcatcca gcggttgcac ctgtaccttg
     2521 tgcggaacga agcgctcggc tgcggtgtgc agccagacca cgaggtcgcc cgatggcgcg
     2581 cgtgccgcgg cggtggacgg aatgaccacc ccgcgtttct tctccttgag ctggacgaac
     2641 accttcaccg gctggccgac cgccagcgcc ggcgccggtt gcacaatgcg cagctggacc
     2701 ggcagggcct gctcgcgcag ttcgaggccg gtgccgagca agttcagctt caagggctcg
     2761 ccgctctccg t
//
LOCUS       DATLGF010000340         6785 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_46586,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000340 DATLGF010000000
VERSION     DATLGF010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6785
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46586"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..810
                     /gene="lpxA"
                     /locus_tag="VM406_10315"
     CDS             22..810
                     /gene="lpxA"
                     /locus_tag="VM406_10315"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HVL76402.1"
                     /translation="MTRIHPTAVVDPAAELDSTVEVGAFAVIGPNVRIGARTRIGPHV
                     VVEGHTTIGCDNHFLQYSSIGAQPQDKKYAGEPTRLEIGDRNTVREFCTFNTGTVQDA
                     GVTRIGNDNWIMAYVHIAHDCQLGSHLILANNATLGGHVHLEDWVFLGGMTTVHQFCR
                     IGMHAMTAFTAAVTQDVPPFVTAAGNRAGPVGINSEGLKRRGFSPEQITAIRRAYKLI
                     YRSNLSLDEAKSALAREEADAPESAVHLRRLREFLENNTRGIIR"
     gene            824..1960
                     /gene="lpxB"
                     /locus_tag="VM406_10320"
     CDS             824..1960
                     /gene="lpxB"
                     /locus_tag="VM406_10320"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HVL76403.1"
                     /translation="MVAGESSGDLLASRLLAGLRPHLPDALIHGIGGSRMAEYGFRSD
                     WPIEKLSVRGLFEVLAHYREIKGIQNALRDALLRSRPSVFIGVDAPDFNFGLEAQLKQ
                     AGIPTVHFISPSVWAWRGGRIKKIARAVSHMLVVFPFEEALYREAGIPVTYVGYPLAE
                     TIPLQPDVAAARRALGIASDATVVALLPGSRMSELKYNTAAFVGAAAKLAQRDRTIRF
                     VVPMAGERQRAYFSELVAALGLDRVEMDVLPGRSHEAIAAADAVLVASGTATLEVALY
                     KKPMVIAYKMMRASWEILRHMRYQPWIGLPNILAQDFLVPEFLQHEATPDALAQALWR
                     QLSDVPARDRLQQRFVEMHHSLLRDSARESASAVLRVIENGGRP"
     gene            1969..2604
                     /gene="rnhB"
                     /locus_tag="VM406_10325"
     CDS             1969..2604
                     /gene="rnhB"
                     /locus_tag="VM406_10325"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HVL76404.1"
                     /translation="MKGQAQSLPLFDHAGEVICGVDEAGRGPLAGAVYAAAVILDPAR
                     PIDGLRDSKKLPEARRDELALCIKENALAWSIAFCSEAEIDTLNILQASLLAMRRAVE
                     GLKVAPTLALVDGNQCPVMRVRSEAVIKGDDTVPEISAASILAKTARDAALRELHLLY
                     PHYGFDQHKGYPTELHLERLRLHGVSPVHRRSYAPVKAMLEVVQVQAATAP"
     gene            2601..3431
                     /locus_tag="VM406_10330"
     CDS             2601..3431
                     /locus_tag="VM406_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HVL76405.1"
                     /translation="MKSITSRDNPRYKELKQLASSASARRKTATTLLDGIHLCQSYLE
                     HVGQPVSCVLSESALGHPEVQPVVAQCEDAGADCLVLSDALYAGLSQVEHGVGILFVA
                     RVPQPPRPARLDGDAVLLDGLQDPGNLGSILRSAAAAGIEHVYCGRGTAAAWSPKVLR
                     AGMGAQFLLRIHEDVELEPLIANARVPVLATSSHAAQQIYDADLTRPVAWLFGNEGRG
                     VAPHLLAQAALQIGIPQPGPIESLNVAASAAVCLFEQVRQRLRDTPTRAPDRPRPARR
                     "
     gene            complement(3325..3705)
                     /locus_tag="VM406_10335"
     CDS             complement(3325..3705)
                     /locus_tag="VM406_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76406.1"
                     /translation="MELLILEIIWWAVLFFFFWALRDARGRLQADLEAMALVNDDRSG
                     RPRLARFQRPQNMRHAIGSYQGQTIYRFVDVDGRTYEFDRACPPECAASVEPDEVYLA
                     PGLVYREAAGAPVRTDRLPQMPRH"
     gene            complement(3823..4671)
                     /locus_tag="VM406_10340"
     CDS             complement(3823..4671)
                     /locus_tag="VM406_10340"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HVL76407.1"
                     /translation="MPDTSSPHFPAADRTVFFVSDGTGITAETFGHSVLSQFELRFKQ
                     VRLPFIDTLHKAYEAVQEINDACEKDGKRPIVFSTLVKSDLSSVVRQSRGMHMDLIQT
                     FVEPLEQELGVKSTHTIGRSHNIADSESYKNRIEAINFTLAHDDGQSHQNLASADVIL
                     VGVSRSGKTPTSLYLAMQYGIKAANYPLIPEDFERGKLPSGLLPFRNKIFGLSIAPDR
                     LAEVRNERRPGSKYAAIENCRYEVSEAEKMMRREGIRWTSSTTKSIEEIAAMILQEIK
                     ADKRVF"
     gene            4898..>6785
                     /gene="ppsA"
                     /locus_tag="VM406_10345"
     CDS             4898..>6785
                     /gene="ppsA"
                     /locus_tag="VM406_10345"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HVL76408.1"
                     /translation="MSNATTMGVAQGAAYVAAFEDLRMTDVESVGGKNASLGEMISQL
                     AGAGVRVPGGFATTAAAFRDFLAYSTGGSTLGERIAERLAKLDVDDVRALAQAGAEIR
                     QWIVETPFQPRLEEEIRAYYERLVAGSASEVSFAVRSSATAEDLPDASFAGQQETFLN
                     VVGIDNVLEAIKHVFASLYNDRAMSYRVHKGFTHSEVALSAGVQRMVRSDVGAAGVMF
                     TIDTESGFKDVVFITSSYGLGETVVQGAVNPDEFYVHKPMLEQGKMPIIRRNIGSKLI
                     KMEFTGEAKAGRSVKTVDVPIELRNRYSLNDEEIVELAKYAVIIEKHYGRPMDIEWGK
                     DGRDGKLYILQARPETVKSQQKAGDAQQRFKLKGSGTVLASGRAIGQKIGAGPVRVIR
                     DPSEMERVQPGDVLVADMTDPNWEPVMKRASAIVTNRGGRTCHAAIIARELGVPAVVG
                     CGNATDMLKDGTLVTVSCAEGDEGRIYDGLLETEVSEVARGELPQIPLKIMMNVGNPQ
                     LAFDFQAIPNGGVGLARLEFIINNNIGVHPKAILEYPNVDTDLKKAVESVARGHASPK
                     AFYVDKLAEGVATIAAAFWPKPVIVRLSDFKSNEYKKLIGGSRYEPDEENPMLGFRGA
                     ARYLA"
BASE COUNT         1215 a         2104 c         2271 g         1195 t
ORIGIN      
        1 cagccggcgg gacagtagcc aatgaccaga attcatccga ccgcggtcgt cgatccggca
       61 gcagaactcg attcgacggt ggaagtgggc gcgttcgccg tcatcgggcc gaacgtgagg
      121 atcggcgcgc gcacgcgcat cggcccgcac gtggtggtcg aaggacacac cacgatcggc
      181 tgcgacaatc atttcctcca gtactcctcg ataggcgccc agccgcagga caagaagtac
      241 gccggcgaac cgacgcggct ggagatcggc gatcgcaaca cggtgcgcga attctgcacg
      301 ttcaataccg gtaccgtgca ggatgcgggc gtcacgcgga ttggcaatga caactggatc
      361 atggcgtacg tgcacatcgc gcacgattgc cagctcggca gccatctgat tctcgcaaac
      421 aatgcgacgc tgggcggaca tgtgcacctg gaggactggg tctttctcgg cggcatgacg
      481 actgtccatc agttctgccg catcgggatg catgcgatga cggctttcac tgccgccgtc
      541 acccaagacg tgccgccgtt cgtgaccgcc gcgggcaacc gcgccgggcc ggtcggcatc
      601 aacagcgaag gcctgaagcg gcgcgggttc tcgcccgagc agatcaccgc catccggcgc
      661 gcatacaaac tcatctaccg ttccaatctc tcgctggacg aggcgaagtc cgccctcgcg
      721 cgcgaggagg cggacgcccc ggaatcagcc gtgcacctgc gccggctgcg cgaattcctc
      781 gagaacaata cccgtggaat catccgctaa ggtgtcggtc gcaatggtcg ccggggagag
      841 ttccggcgac cttctggcga gccggctcct ggccggactg cgtccgcact tgcctgacgc
      901 gctgatccac ggcatcggcg ggtcgcgcat ggccgagtat ggtttccgca gcgactggcc
      961 aatcgaaaag ctgtcggtgc ggggcctgtt cgaggtgctt gcccactatc gcgagatcaa
     1021 ggggatccag aacgcgctgc gtgacgcgct gctgcgttcg cgcccctcgg tgttcatcgg
     1081 tgtcgacgcg ccggatttca actttggcct ggaggctcag ctcaagcagg ccggcatccc
     1141 gaccgtgcat ttcatcagcc cgtcggtctg ggcatggcgg ggcggacgca tcaagaagat
     1201 tgcgcgcgcg gtctcgcaca tgctggtggt cttcccgttc gaggaggcct tgtatcggga
     1261 agccggcatc ccggtgacct atgtcggcta tccgttggcc gagacgattc cgctgcagcc
     1321 tgacgtggcc gctgcccgcc gtgcgcttgg cattgcgtcc gatgccaccg tggtggcgct
     1381 gcttccgggc agccgcatgt ccgagctcaa gtacaacacg gccgcattcg tcggcgctgc
     1441 cgcgaagctc gcgcagcgcg accgcacgat acgcttcgtg gtgccgatgg ccggcgagcg
     1501 ccagcgcgcc tacttcagcg agctcgttgc cgcgctgggg ctggatcgcg tcgaaatgga
     1561 cgtgctgccc ggccgctcgc atgaggcgat tgccgccgcc gatgcggtgc tggttgcttc
     1621 cggcaccgcg acgctcgagg tcgcgctgta caagaagccc atggtcatcg catacaagat
     1681 gatgcgcgcc tcgtgggaaa tactgcgcca catgcgctac cagccctgga tcggcctgcc
     1741 caacatcctg gcccaggact ttctggttcc cgaattcctg cagcatgaag cgacgccgga
     1801 tgcgctcgcg caggccctgt ggcggcagct gagcgatgta ccggcgcgcg accggctgca
     1861 gcagcgtttc gtcgagatgc accacagcct tttgcgcgac agcgcccgcg agagcgccag
     1921 cgcggtgctg cgcgtcatcg agaacggcgg gcggccatga ggaaggggat gaaggggcag
     1981 gcccagagcc ttccgctgtt cgatcatgcc ggggaagtca tctgcggcgt ggacgaagcc
     2041 ggtcgcggcc cgttggccgg cgcggtgtat gcggccgcgg tcattctgga tccggcgcgt
     2101 ccgatcgacg gattgcgcga ttcgaagaaa ctgcccgaag cgcgccgcga cgaacttgcg
     2161 ctgtgcatca aggagaacgc gctggcatgg tcgatcgcat tctgctcgga agcggaaatc
     2221 gatacgctca atatcctgca ggcctctttg ctggcgatgc ggcgggcggt cgaggggctg
     2281 aaggtggcgc cgacgctggc gttggtggac ggcaaccagt gtccggtcat gcgcgtgcgc
     2341 tcggaggcgg taatcaaggg ggatgacacg gtacccgaga tttcggcggc ctcgatactc
     2401 gccaagaccg cgcgcgatgc ggccctgcgc gagctgcatc tgctgtaccc gcactacggt
     2461 ttcgaccagc acaagggcta cccgaccgag ctgcatctcg agcgcctgcg cctgcacggg
     2521 gtctcgccgg tccatcgcag atcgtacgcg cccgtcaagg ccatgctcga agtcgtgcag
     2581 gtgcaggcag cgacggcacc atgaaatcga tcacctcgcg cgacaatccg cgctacaagg
     2641 aattgaagca gctggcttca agtgcatcgg ccaggcgcaa gactgcgacc acgctgcttg
     2701 acggcattca cctgtgccag agctacctcg agcatgtggg ccagccggtc agctgcgtgt
     2761 tgagcgaatc ggcgctcggg catcctgagg ttcagcctgt cgttgcgcag tgcgaggatg
     2821 cgggcgccga ctgcctggtg ttgagcgatg cgctgtatgc cggcttgagc caggtcgagc
     2881 atggggtcgg aatcctgttc gtggcacggg tgccgcagcc gccgaggccg gcgcgtctcg
     2941 atggcgacgc cgtgctgctc gatggtcttc aggacccggg gaacctgggc tcgatcctgc
     3001 gcagcgcggc ggcagccgga atcgagcatg tgtactgcgg acggggaacg gcggccgcgt
     3061 ggtcgcccaa ggtcttgcgc gccgggatgg gcgcgcagtt cctcctgcgg attcatgaag
     3121 acgtggaact ggagccgctg attgcaaacg ctcgggttcc ggtgctggcc accagctcgc
     3181 atgcggcgca gcagatctat gacgcggacc tgacgcggcc ggtggcctgg ctgttcggca
     3241 acgaggggcg cggagtggcg ccgcatctgc tcgcgcaggc ggcactgcag atcgggattc
     3301 ctcaacccgg accgatcgag tcgctcaatg tcgcggcatc tgcggcagtc tgtctgttcg
     3361 aacaggtgcg ccagcggctt cgcgatacac caacccgggc gccagataga cctcgtccgg
     3421 ctcgacgcta gcggcgcact cgggcgggca ggcccggtcg aattcgtagg tgcgtccgtc
     3481 gacgtcgacg aatcggtaga tcgtctggcc ctggtagctt ccgatcgcgt ggcgcatgtt
     3541 ctgcggccgt tgaaagcgcg ccagtcgggg gcgtccggaa cggtcgtcgt tcaccagggc
     3601 catcgcctcc aggtcggcct ggagacggcc acgcgcgtcc cgcagtgccc aaaagaaaaa
     3661 gaacaggacg gcccaccaga taatctccag aatcagcagt tccatctccg gtcccgcctc
     3721 cttccacggc tgtcgaattg cgtaagaaat atgcaattcg acagctatcc ggaagctttc
     3781 gttctcccgt ttgcggcaaa tccggcaggc ggagcagggc cctcagaaga cgcgtttgtc
     3841 ggccttgatc tcctgcagga tcatcgcggc gatctcctcg atcgacttgg tcgtcgacga
     3901 agtccagcgt atgccttcgc gccgcatcat cttctccgcc tcgctcactt cgtagcggca
     3961 gttctcgatg gccgcgtact tgctgccggg ccggcgctca ttgcgcactt ccgcgagccg
     4021 gtcgggtgcg atcgacaggc cgaagatctt attgcggaag ggcaacaggc cgctcgggag
     4081 cttgccgcgc tcgaaatctt ccgggatcaa cggatagttg gcagccttga tcccgtactg
     4141 catcgccagg tacaggctgg tcggcgtctt gccggaacgg gacacgccga ccaggatcac
     4201 atccgcagac gccaggttct ggtgcgactg gccgtcgtcg tgggcgaggg tgaaattgat
     4261 cgcctcgatg cggttcttgt agctctcgga atcggcgatg ttatggctgc gcccgatcgt
     4321 gtgggtcgac ttcacgccga gttcctgctc cagcggctcg acgaaggtct ggatcaggtc
     4381 catgtgcatg ccgcgggact ggcgaaccac cgaggacagg tctgacttga ccagcgtgga
     4441 aaagacgatc ggccgcttgc catccttctc gcaggcgtcg ttgatttcct ggaccgcctc
     4501 atacgccttg tgtagcgtgt cgatgaacgg gagccgcacc tgcttgaagc gcaactcgaa
     4561 ctggctgagg acggaatggc cgaatgtctc ggccgtgatg cctgtgccgt cggacacgaa
     4621 gaagacggtg cggtctgctg cggggaagtg cggggatgag gtgtctggca tcgggaggtt
     4681 agctgaaaat catgaaaagc ttgaaattgt gggccgcaag aaccccttgc agacggtaaa
     4741 atggcgctca attgaatcac caccctggcc cggttccggt caagacgacg tcggagtccc
     4801 cgggcgcagc gctcccaaga ataacaaaaa gagcagcgcg agaagttgta cacaagggcg
     4861 aacgtgcaga tttcttatca tcaaaacaga ggtagtcatg tccaacgcaa caacgatggg
     4921 ggtcgcccag ggggctgctt acgtagccgc atttgaggat ctcaggatga cggacgtcga
     4981 gtccgtcggc ggaaagaacg cgtcgctcgg agaaatgatc agccagctgg ctggcgccgg
     5041 cgtacgggtg cccggcggtt tcgctaccac ggcggccgct ttccgcgatt ttcttgcata
     5101 cagcaccggc ggttccacgc tgggcgagcg catcgccgag cggctcgcca agctggacgt
     5161 ggatgacgtt cgggcgcttg cccaggccgg cgccgagatc cgccaatgga tcgtcgaaac
     5221 ccctttccag ccgcggctcg aagaggaaat ccgcgcctac tatgagcgcc tggtggccgg
     5281 ctccgcttcc gaggtgtcat tcgcggtgcg ctcgtcggca accgccgagg acctgcccga
     5341 tgcctccttc gccggccagc aggagacctt cctgaacgtg gttggcatcg acaatgtgct
     5401 cgaggcgatc aagcacgtgt tcgcctccct ctacaacgac cgcgcgatgt cgtatcgcgt
     5461 gcacaagggc ttcacccatt ccgaggtcgc gctgtcggcc ggcgtgcagc gtatggtgcg
     5521 gtccgacgtc ggcgctgccg gcgtgatgtt caccattgat accgagtccg gcttcaagga
     5581 cgtggtgttc atcacctcca gctatggcct gggcgagacg gtggtgcagg gcgcggtgaa
     5641 cccggacgag ttctacgtgc acaagccgat gctcgagcag ggcaagatgc ctatcatccg
     5701 ccgcaacatc ggctccaagc tgatcaagat ggagttcacc ggcgaggcca aggcgggccg
     5761 ctcggtgaag acggtcgacg tgccgatcga gctgcgcaac cggtattcgc tcaacgatga
     5821 agagatcgtc gaactggcga agtacgcggt gatcatcgag aagcactatg ggcgcccgat
     5881 ggacatcgag tggggcaagg acggccgcga cggcaagctg tacatcctgc aggcgcgccc
     5941 ggagaccgtg aagtcgcagc agaaggccgg cgatgcccag cagcgcttca agctcaaggg
     6001 cagcggaacc gtgctggcgt ccggccgcgc aatcggccag aagatcggcg ccggcccggt
     6061 acgggtgatc cgcgatccgt ccgagatgga gcgggtgcag ccgggcgatg tgctggtggc
     6121 cgacatgacc gacccgaact gggaaccggt gatgaagcgc gcgtcggcga tcgtgaccaa
     6181 ccgaggcggc cggacctgtc acgcggcgat cattgcgcgc gagctgggcg tgcccgccgt
     6241 ggtgggttgc ggcaacgcga ccgacatgct caaggacggc acgctggtca cggtctcgtg
     6301 cgccgaaggt gacgaagggc gcatctatga tggcctgctg gagaccgagg tcagcgaggt
     6361 cgcgcgcggg gagctgccgc agattccgtt gaagatcatg atgaacgttg gcaatccgca
     6421 gctggctttc gatttccagg cgatcccgaa tggcggcgtc ggccttgccc gtctcgagtt
     6481 catcatcaac aacaacatcg gcgtccatcc gaaggcaatt ctcgagtacc cgaacgtgga
     6541 caccgacctg aagaaggcgg tggaatcggt cgcgcgcggc catgcctcgc cgaaggcctt
     6601 ctatgtcgac aagctggccg agggcgtggc cacgatcgcg gccgcattct ggccgaagcc
     6661 ggtcatcgtg cgcctgtcgg atttcaagtc caacgagtac aagaagctga tcggcggctc
     6721 gcgctatgag ccggacgagg aaaacccgat gctcggcttc cgcggcgcgg cccgctacct
     6781 ggccg
//
LOCUS       DATLGF010000341         2891 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_46603,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000341 DATLGF010000000
VERSION     DATLGF010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46603"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VM406_10350"
     CDS             <1..321
                     /locus_tag="VM406_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76409.1"
                     /translation="PESRPTDEILSQYHRYHVQITLVDRSAKELRVIRRMTMSHELSV
                     LFNEIVAYQLGNHIGREEYDAQVSIYQNKFPDLQTVVDAADRFEQLEAAPDDPEMAAR
                     VRNR"
     gene            complement(427..882)
                     /locus_tag="VM406_10355"
     CDS             complement(427..882)
                     /locus_tag="VM406_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76410.1"
                     /translation="MPSPLPFPIRVECPPGACNCNREALLADPDADIRVLRLTREEEK
                     KLIARIERISSYEDLKHVARLIEANLGIELRIAPGPNEVRTVRGFVIQLAERPGLCRK
                     VRQSIPAAVRRCLDEKPEIAYAILDAHDLLGGGMVEDAGGAAEPAGRTG"
     gene            complement(1145..1855)
                     /locus_tag="VM406_10360"
     CDS             complement(1145..1855)
                     /locus_tag="VM406_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL76411.1"
                     /translation="MKTLLRVDQLETAYGSSQVLFGVSFDIREGEVATLLGRNGVGKT
                     TTLRSILGLTPARRGAIEFLGQRIERLPADRIARLGIGIVPEGRQIFPNLSVRENLVA
                     FAGNRSGSKDPWTLERVYAMFPRLQERANNMGNQLSGGEQQMLAIGRALMTNPHLLIL
                     DEATEGLSPIIREEIWNCLSQLRGQGQTILVIDKYVERLLALADRHTVIERGRVVWSG
                     SSEALRAEPQVWERYVGV"
     gene            complement(1852..2613)
                     /locus_tag="VM406_10365"
     CDS             complement(1852..2613)
                     /locus_tag="VM406_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL76412.1"
                     /translation="MNPLLELRDLSKRFGGVVATDRLNLTVERGEIHAIIGPNGAGKT
                     TLIHQISGSMKPTAGSVWFDGGDITRLAMHERVARGLARSYQITNVFKSYSVLDNLAL
                     AVQARSGSSMRFWKPALSEQAIFDEAAAMARRVGLGERTHVLGSALSHGEQRQLEVGL
                     ALAAQPKLLLLDEPMAGMGQDESARLIPLIRGLVPGIAVVLIEHDMDAVFQLADRISV
                     LVAGRVIATGNAEEIRRNADVKKAYLGDEVAGVTA"
     gene            complement(2610..>2891)
                     /locus_tag="VM406_10370"
     CDS             complement(2610..>2891)
                     /locus_tag="VM406_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVL76413.1"
                     /translation="ALAGALLANQNMSVSPNVLHWTQSGALMVMVILGGVGYLWGGVI
                     GAVVFLMLEEVLAGYTIHWQLALGLVLLAVVLVLPNGIASLFARLRRTS"
BASE COUNT          501 a          972 c          939 g          479 t
ORIGIN      
        1 ccggagagcc gcccgaccga cgaaatcctg tcgcagtatc accgctacca cgtgcagatc
       61 acgctcgtgg accgcagcgc gaaggagctg cgcgtgatcc ggcgcatgac gatgtcgcac
      121 gagctcagcg tgctgttcaa cgagatcgtt gcctaccagc tcggcaacca tatcggccgg
      181 gaagagtatg acgcgcaagt cagcatctac cagaacaagt ttcccgatct gcagacggtg
      241 gtcgacgccg cggaccgctt tgagcagctc gaggcggcgc cggacgatcc tgaaatggcg
      301 gcccgcgtgc gaaatcggta agcagagagg gcagccaccg ggtcgacggt ggtgaacaac
      361 ggggaagcat gttgtcccga ggagcaaggc gaagcgggac ctcatcgatc tcgccgggcg
      421 acgatgctac ccggtccgac ccgccggctc ggcagcgccg cccgcatcct ccaccatccc
      481 tccaccaagc agatcgtgcg catcgaggat cgcataggcg atttccggct tttcgtccag
      541 gcagcgccgc accgccgccg gtatcgactg gcgcaccttg cggcacaagc ccggccgctc
      601 cgccaactgg atgacaaagc cgcgcacggt gcgcacctcg ttcggccccg gcgcgatgcg
      661 cagctcgatg ccgagattgg cctcgatcag gcgcgccaca tgtttcagat cttcatagct
      721 ggaaatgcgc tcgatgcgag cgatcagctt cttctcctcc tcgcgcgtga ggcgcagcac
      781 gcggatatcc gcatccgggt cggccagcag cgcctcccgg ttgcagttgc aggcaccggg
      841 cgggcattcg acgcggatgg gaaagggcag gggggacggc atcgtcagaa acggtggggt
      901 cttgaatgcg cccctagtct gccatggaat cggtcgccgg taggcgaacg ggatcgggaa
      961 atgcgcttac tcaagttttg tcgctcccct aggcggggct ccgctcaggc aggagcccag
     1021 ctccagcgct cgcagacgtg cgcacccgcg atccggattc cccctgtttc ggcacaacaa
     1081 aatcccactt gcgtgcagcg actttgaggc agatccacaa acagggcagt cccgaaggca
     1141 tgactcacac ccccacgtag cgctcccaca cctgcggttc ggcccgcagt gcctcgctgc
     1201 tgcccgacca tacgacgcgc ccgcgctcga tcaccgtgtg gcggtcggcg agcgcaagca
     1261 gccgttcgac gtacttgtcg atcacgagaa tcgtctggcc ctggccgcgc agctgcgaca
     1321 ggcagttcca gatttcctcg cggatgatcg gggacaggcc ctcggtggcc tcgtcgagga
     1381 tcagcaggtg cgggttggtc atcagcgcgc ggccgatcgc gagcatctgc tgttcgccgc
     1441 cggagagctg gttgcccatg ttgttcgcgc gttcctgcag gcgcgggaac atcgcgtaca
     1501 cgcgttccag cgtccacgga tccttgctgc cgctgcggtt gccggcaaac gcgacgaggt
     1561 tctcgcgcac ggaaaggttc ggaaagatct ggcggccttc gggcacgatg ccgatgccga
     1621 gccgggcgat gcggtctgcg ggcaggcgtt cgatgcgctg gccgaggaat tcgatcgcgc
     1681 cgcgccgggc cggcgtgagg ccgagaatcg agcgcaatgt ggtggtcttg ccgacgccgt
     1741 tgcgtccgag cagggtcgcc acctcgcctt cacggatgtc gaaactcacg ccgaacagca
     1801 cctggctgct gccataggcg gtctcgagct ggtcgacgcg cagcaaggtc ttcatgcggt
     1861 cactcccgcg acttcgtcgc cgagataggc cttcttcacg tcggcgttgc gccggatttc
     1921 ttctgcgttg ccggtcgcga tcacgcgccc ggcgaccagc accgagatgc ggtcggccag
     1981 ctggaacacc gcgtccatgt cgtgttcgat caacaccacc gcaattcccg gcaccaggcc
     2041 cctgatcagc gggatcagcc gcgccgattc gtcctgtccc atgccggcca tcggttcgtc
     2101 gagcagcagc agcttgggtt gggcggccag cgccagtccg acttccagct ggcgctgctc
     2161 gccatgcgac agtgcgctgc cgagaacatg cgtgcgttca cccagcccga cgcgccgcgc
     2221 catcgcggcc gcctcgtcga agatcgcctg ttccgacagc gccggcttcc agaaacgcat
     2281 gctggagccg gaacgtgcct gcaccgccag cgccaggttg tccagcaccg agtacgactt
     2341 gaagacgttg gtgatctggt acgagcgtgc cagcccgcgg gcgacgcgtt catgcatcgc
     2401 gagccgggtg atgtcgccgc cgtcgaacca gacgctgccg gccgtcggct tcatgctgcc
     2461 ggaaatctgg tggatcagcg tggtcttgcc ggcgccgttg gggccgatga tcgcgtggat
     2521 ttcgccgcgc tcgacggtga ggttcaggcg gtcggtggcg accacgccgc cgaagcgctt
     2581 ggacaggtcg cgcagttcga gcaagggatt catgacgtcc tccgcaggcg ggcgaacagg
     2641 ctggcgatgc cgttgggcag caccagcacc acggccagca gcaccaggcc gagcgcgagt
     2701 tgccagtgga tcgtgtagcc ggccagcact tcttcgagca tcaggaacac gaccgcgccg
     2761 atcacgccgc cccacaggta gccgactccg ccgagaatca ccatcaccat cagcgcgccg
     2821 gactgcgtcc agtgcagcac attcgggctc acgctcatgt tctggttggc cagcagcgca
     2881 ccggcaagcg c
//
LOCUS       DATLGF010000342        19241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_60787,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000342 DATLGF010000000
VERSION     DATLGF010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 19241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19241
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60787"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1288)
                     /gene="gspF"
                     /locus_tag="VM406_10375"
     CDS             complement(68..1288)
                     /gene="gspF"
                     /locus_tag="VM406_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HVL76414.1"
                     /translation="MPAFRYEAVDDAGATRKGVLNADSARAARSDLRAKGLVPIAVDA
                     IAAQVDEFGQARPRAFGDRLSTVEQALFTRQLASLLEASLPLEQAFTALLEQAERPYM
                     RDLIASIRSELMGGSSLSDALARHPRDFPDIYRALVASGEQIGQLARVLSRLADYIER
                     RNALVQKVRLAFTYPAIVTVVAFAIVIFLLAYVVPQIVSVFAQTKQRLPMLTVIMLAI
                     SDFVRAWGWIVLTGMVAAFFAWRAALRNPDIKYRWHRWLLNAPLYGKFERSLNTARFA
                     STLAITTGSGVPILRALQTSRDTLSNVAMRDQVEEATNAVREGVGLARALSAQQHFPP
                     MLIHMIRAGEVTGELPAMLERASNAQEQDLERRAMTIAGLLEPALILVMGVVVLLIVL
                     AVLMPIIEINQLVR"
     gene            complement(1295..2740)
                     /gene="gspE"
                     /locus_tag="VM406_10380"
     CDS             complement(1295..2740)
                     /gene="gspE"
                     /locus_tag="VM406_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HVL76415.1"
                     /translation="MSAVTDARLLPFAFARDHLLLARRAEQPGNAVELWVSEGSAPSA
                     IAEVSRRFGRVKLKSLSREELEAAIARAYANSGGDAAQVVDEVESDLDLAKLMLDMPA
                     VEDLLESADDAPVIRMINALLTQALREEASDIHIEPFEQISVVRFRVDGALRDVVRPK
                     KAIHASLISRIKIMAQLDIAEKRLPQDGRITLRVGGKPVDVRVSTLPTGHGERAVLRL
                     LDKEGGRLDLSHLGMSPDVQQQFDHLIAQPHGIVLVTGPTGSGKTTTLYAALSRLNSP
                     TSNILTVEDPIEYELQGIGQTQVNPRIDMTFAKALRAILRQDPDVIMIGEIRDLETAQ
                     IAVQASLTGHLVLATLHTNDSAAAVTRLLDMGIEPFLLSSSLLGVVAQRLVRKLCPHC
                     KQPDPHGGPEWQAVGCDKCGHTGFHGRVGIYELLLTTEEIRAQIHDQASEADIRQAAQ
                     RNGMRTMREDGERWIADGTTTRAELLRVAKE"
     gene            complement(2737..4896)
                     /gene="gspD"
                     /locus_tag="VM406_10385"
     CDS             complement(2737..4896)
                     /gene="gspD"
                     /locus_tag="VM406_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HVL76416.1"
                     /translation="MLCALGTVTPPALTQEPTASEATLNFVGADIESVIKAVGHYTRT
                     TFIIDPRVKGTITLVSERPVTRAQALQLLASTLRLQGFAMVRTDGFTKIVPEADAKLQ
                     AAPVQIPEVRGDQIATQIFRLNHESANNLVQILRPLISPNNTINVNPGNNSLVITDYA
                     DNLRRLGRIIATLDAPAVGDLDVIPIQHAIATDIAQMVNRLLDQGAAPAAGGEAGRMT
                     LLADPRTNSVIVRAPSAARANLARSLVARLDQPTAQPGNVHVVYLKNADAVRLAQTLR
                     AVVAGETGAQPGAAQQQQPPQQQQQPQQQQAALGQGQLPQQTTPTALPAGGPAGFIQA
                     DPATNSLIITASESVYRNLRAVIDQLDARRVQVYVESLIVELTADRAAELGVQWAGLS
                     GTNNSDYRVGVISGFGQAGNNLVNQVVSRAATGNPLPPGNGINLGIFRQINGQLTLGA
                     LARALESEGNANILSMPNLITLDNEEARIVVGQNVPFVTGQFTTQAGGANVNPFQTIE
                     RQDVGLSLRVRPQVSEGGTVRMQIYQETSNLQPTTNAADIITNKRSIETNVLVDDGEI
                     IVLGGLIEDRVTDNVEKVPGLGDVPVLGNLFRYQSRRGTKTNLMVFLRPTVIRTNEQS
                     VHVSGDRYDYIRNMQILRQPAPAFGLPQIDAPILPPSESGQVNAAPLVLPPPRPLPEG
                     AEPQPVPTQPLQPLPAPTPLPPGSQVPEIDLRPKQTQ"
     gene            complement(4968..5741)
                     /locus_tag="VM406_10390"
     CDS             complement(4968..5741)
                     /locus_tag="VM406_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HVL76417.1"
                     /translation="MRRLVIWLLAGALSIALTVIVFFPAAWLAPAVEAQTGGRLTLGD
                     AQGSIWRGSAFIGGAPSGNDPVTPLLPGRFEWRLSPLVLLGQVDAELRNPDSLSQPVS
                     ISGSWWDWQVSPAHVMLPAERLAGLGAPLNTIQPSGTMQLSWTPLQMTRRTNTVDLHG
                     TMTLQLENVSSRLSPVRPLGAYRMKMDWRGQQAQLALTTIKGPMLLSGNGTLINGRLQ
                     FSGKAEAEAGQEDRLANLLNLLGQRRRENGKDVIALEFK"
     gene            complement(5770..6279)
                     /gene="gspM"
                     /locus_tag="VM406_10395"
     CDS             complement(5770..6279)
                     /gene="gspM"
                     /locus_tag="VM406_10395"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HVL76418.1"
                     /translation="MNRLQAMRQSAVAYWNERNARERTMLAAAAGVLVLALIYLLLLD
                     PALSGREELAQRLPALRQQAAEVQALTREAAAAGSRTAATAPAVTRETLQTSLSRRGL
                     QAQEISVSGELVRLRFEGASFAAQTEWLAEMHRTARLAVVEAKVEAGGEPDSVNAAYT
                     LRQERGQAQ"
     gene            complement(6276..7529)
                     /gene="gspL"
                     /locus_tag="VM406_10400"
     CDS             complement(6276..7529)
                     /gene="gspL"
                     /locus_tag="VM406_10400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HVL76419.1"
                     /translation="MSTLFIRLPAHAGAGSLQPGMPLYCAYAIASDADRIEREGIAAL
                     SELGDAVKRVRQVVLLLAASDVTLLRIKMPPLTGARLKAALPNLVEDHLMSDPAECVV
                     VAGELKDGLRTVGVVQRSWLEILSRTLTTLGARKLSAVPAQLCLPIAGEAAAAAVAEH
                     GIDIDLTVRMGAQEGMGVSVLADPPESAPFEVIQSLNALVPQAPVVLYVPPAKLHDYQ
                     GSLHLVPALEQRITLEEDNWSIWIAGARQAGLDLISGLSGASGPKIDWRRLRWPVALA
                     AGLVLVNLIGLNVDWMRMQREAETLRASMLQNYRSAFPQDTVIVDPLAQARQKAAAAR
                     NASGELAAGDFLALSAGLAEAWQAIGGTGGAPVASLDYRERTLTVRMKPGSGVDPEAL
                     NTALASRNLDVSEQGEGVLQIRSTR"
     gene            complement(7543..8499)
                     /gene="gspK"
                     /locus_tag="VM406_10405"
     CDS             complement(7543..8499)
                     /gene="gspK"
                     /locus_tag="VM406_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HVL76420.1"
                     /translation="MRLRSRQSQRGVAIVTALLLTTLAVTIVGSLFWQQQVQVRSIEN
                     QRMQLQKQWILRGALDWAQLILRADGAMTAEDHLGEPWAVPLAETRLDEYVDNPRANP
                     EASDAVLSGSIVDAQSRFNLNNLAANGTINPQELAAFRRLLVNLGLNAGLAQAAADLV
                     AAGQRAPGGQGAAGGQQAAGGAQMRVEHVDDLLAAPGFTPDIVARLSEFVVVLPDLRA
                     INVNTAPAEVLSARIERLSMSDAAALVAARENAWFRSTNEFVDRLRQMGMGGAAVQHI
                     DVRTEYFLINGHVRMSRAAMDMQALIRRPGRAEPSLIWIREY"
     gene            complement(8496..9107)
                     /locus_tag="VM406_10410"
     CDS             complement(8496..9107)
                     /locus_tag="VM406_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HVL76421.1"
                     /translation="MNIRLRRARGFTLIELLVAISVLAIVAVMGWRGLDTIIRAREGL
                     NRDLEQTRGLQLTFAQMQNDCDKIALPQAIGNRPVVDAGNGRLTLVRMVYAENQPSRV
                     QVVSYRLQDGVLSRRESLPTRDVNLLAAAWTAALGDADPTPPVVLHRSVQNMNLRLWY
                     QNVGWRQSGTPAPGNAPLIGVEVGLDLVERPGTMFKIFMLGNA"
     gene            complement(9104..9490)
                     /gene="gspI"
                     /locus_tag="VM406_10415"
     CDS             complement(9104..9490)
                     /gene="gspI"
                     /locus_tag="VM406_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HVL76422.1"
                     /translation="MPRRSSLRRSRGFTLLEVLVAMVVVGTALGASLRAVGSLMQNGS
                     ALRASMMATWSAENRLTQLRLSREFPPLGRRSFPCPQAELQLVCQEEVVATPNPFFRR
                     VEVSVSSAEDPERRIIRLTQIVPRRQ"
     gene            complement(9492..10022)
                     /locus_tag="VM406_10420"
     CDS             complement(9492..10022)
                     /locus_tag="VM406_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HVL76423.1"
                     /translation="MGSVTPSCSVPEWRARAPHPAREQGFTLLEILVVMVIAGLLLGV
                     VAFNAMPGERQALQTEAQRIALLLQLARDEAIVRNRAIAFEADSARYRFLEREGNAWI
                     AVPQDDLLRERPFALAPVTLTTSPAAVNAAGASEVQVRLVFGREPVDRPFVLMLSRGD
                     TSVAIRADGIGNFTVE"
     gene            complement(10010..10471)
                     /gene="gspG"
                     /locus_tag="VM406_10425"
     CDS             complement(10010..10471)
                     /gene="gspG"
                     /locus_tag="VM406_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HVL76424.1"
                     /translation="MQTAFSPIRRPLSRTLQRGFTLIEIMVVVVIIGVLAAVVMPKVM
                     GRADDARITAARQDIATLSSALKLYKLDNHRYPTTEQGLQALVQRPTTGPAANGWKSG
                     GYIDKLPRDPWGNSYQYLSPGVRGEFDVFSYGADGQPGGNGNDADIGSWDL"
     gene            10742..11080
                     /locus_tag="VM406_10430"
     CDS             10742..11080
                     /locus_tag="VM406_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVL76425.1"
                     /translation="MSLPHAASGDIIDVRPLGPKLADAVSTALFKTDQLEAMRLVLTT
                     GKEIPEHHVAGDFTLHCIEGKVELRVNADVRTLQAGEMVFVAAEAPYSIRALADASLL
                     MTLARCREGG"
     gene            complement(11087..12118)
                     /locus_tag="VM406_10435"
     CDS             complement(11087..12118)
                     /locus_tag="VM406_10435"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HVL76426.1"
                     /translation="MSVLAAGTNRSRNISILGAGAWGTALGIVLARRHQVILWGRDAA
                     AMREAAAQHENSAYLPGFALPSALRPTADFDAAVAHAATGDGLLIVASSVAGLRPLAE
                     RLKSHPIQNLVWLCKGFEEHTQLLPHQVVAQVLGDALPCGALSGPSFAQEVAQGLPCA
                     LTVASSSAALRTIVVETVHGGNLRVYSSDDLVGVEVGGAVKNILAIATGVVDGLGLGL
                     NARAALITRGLAEITRLGVALGGRSETFMGLTGVGDLILTCTGDLSRNRRVGLGLARG
                     LKLDAVVAELGHVAEGVRCAQAVCALAAAHQVDMPITNAVAAVLFEGDSPAAMVQRLL
                     ARDPREETT"
     gene            complement(12123..12563)
                     /locus_tag="VM406_10440"
     CDS             complement(12123..12563)
                     /locus_tag="VM406_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HVL76427.1"
                     /translation="MSRLALIAAALVAAAGSAHALEYRSVGSSPAILYDAPSAKGRRV
                     FVAPRGMPVEVVLTYGEWTKVRDAAGDLAWVETRALAARRTVVVNAAAARVHQAPDEA
                     SPVVFSASQGVLMDMLEPVNAGWVRVRHADGQGGFVRAGEVWGE"
     gene            complement(12599..13102)
                     /gene="secB"
                     /locus_tag="VM406_10445"
     CDS             complement(12599..13102)
                     /gene="secB"
                     /locus_tag="VM406_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HVL76428.1"
                     /translation="MAEQNQQPVFQIQRIYLKDMSLEQPNSPAIFLEQEAPNIEVAVD
                     VGVEALAETIFESTVTITLTAKIKDKVAFLVEGKQAGIFEAANIPPEQLDPLLGIGCP
                     NIIYPYLRANIADVITRAGFPPVHLAEINFEAFYQQRLQALAQQQQEQGQGAGIVMPD
                     GTAARPQ"
     gene            complement(13200..13463)
                     /gene="grxC"
                     /locus_tag="VM406_10450"
     CDS             complement(13200..13463)
                     /gene="grxC"
                     /locus_tag="VM406_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HVL76429.1"
                     /translation="MTARVLMYSTAVCPYCVMAERLLTSKGVKEIEKIRIDLDPQQRA
                     VMMQKTGRRTVPQIYIGETHVGGFDDLSALDRAGKLDPLLNGG"
     gene            complement(13474..13875)
                     /locus_tag="VM406_10455"
     CDS             complement(13474..13875)
                     /locus_tag="VM406_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HVL76430.1"
                     /translation="MDFLLDNIWLILVAVISGGLLLWPSLKGRGVNVSVLRATQLINQ
                     SNAVVIDVREPSAYAAGHLRDAKNIPLKEMSNRLGDLSKHKSKPVIAVCQSGAQSAKA
                     AAILRKAGFEQAYSLEGGLNAWQAAGLPVSK"
     gene            13980..14726
                     /gene="gpmA"
                     /locus_tag="VM406_10460"
     CDS             13980..14726
                     /gene="gpmA"
                     /locus_tag="VM406_10460"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HVL76431.1"
                     /translation="MYKIVLMRHGESTWNLENRFTGWTDVDLTEKGMAEARQAGRVLK
                     EAGFTFDLCYTSVLKRAIRTLWLTLDEMDLMWLPVVHDWRLNERHYGALQGLNKAETA
                     AKYGDEQVLVWRRSYDVPPMPLDPDDERTSYNDPRYAGLSREQIPLTECLKDTVARVM
                     PAWHDSIAPAIRAGRQILISAHGNSLRALIKDLDGINDADIVGLNIPNGQPLVYELDA
                     NLKPIKSYYLGDQEAIAAAMAAVASQGKAK"
     gene            14733..16136
                     /locus_tag="VM406_10465"
     CDS             14733..16136
                     /locus_tag="VM406_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HVL76432.1"
                     /translation="MRILSGAGRRGTALAAVGLAGLLFCLSAHANKVTERSKQRQAAE
                     AERAELRQKLAALKREIGQTETARTHAADALADSEAAISQANRALRELAQERRDVEAR
                     LAALARDEKRLNQAVAEQRARLSTVLREQYMGGSDDRFKLLLSGDNPNRINRDLQYMG
                     YVSQAQARLLASLQQDLQAIEANRAQAQSARDDLDEIAQEQQASRARLEQEKARRAAL
                     LAELSSRLAAQRKEAGNIERDQQRLSALVDRLARLIEEQRKAEEAARRKQEEQRRQEQ
                     LARARQAEAARQAEAAKASSLNTASRKPDAIDNAPAKVLGRNELTPDARVQAAVPGRA
                     FGSLRGQLRLPVRGELTARYGSTRTDGPSWRGLFIRTAEGEEVKAVAAGQVVFAEWLR
                     GFGNLIIIDHGGQYMTIYGNNQAVLKQVGDTVNTGDVIATAGSSGGSEHSGLYFEMRH
                     QGRTFDPLGWIAPGTLK"
     gene            16186..17661
                     /locus_tag="VM406_10470"
     CDS             16186..17661
                     /locus_tag="VM406_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HVL76433.1"
                     /translation="MGSKLKNFGLIGLGIIAGVAGSMQFEASAQKNLSPLPLEELRQL
                     ADVFGLIKTDYVEPVEDRKLLTEAISGMVASLDPHSSYLDKKAFKELREGTQGKFVGL
                     GIEVGMEDGYVKVISPIEDSPAFKAGLKAGDLITRLDSTPVKGMTLDEAVRRMRGEPN
                     TKITLTIARKEEDRPIIVTIVRQEIRVQSVKSKVVEPGYAWVRIAQFQEPTVDDMVKK
                     LIDIHAKEPNLKGLVLDLRNDPGGVLPGAIGVSAAFLPKDVPIVSTNGQLADSKQTFY
                     ARREFYTSSRLSVGDPLAKLPAIYKDIPMVVLVNTGSASASEIVAGALQDYKRATIMG
                     TQTFGKGSVQTIRQLSPDTAVKLTTARYYTPKGRAIQAKGIVPDLLVDETPEGDGLNG
                     LRLRESDLQKHLSNDRDKEQIKARIDELEEQQRIAALEKRRKPLEFGSTEDFQLAQAL
                     NHLKGLPVQLSKTATIESKKEPTINDPHAGEKNGNGAESKK"
     gene            complement(17752..18246)
                     /locus_tag="VM406_10475"
     CDS             complement(17752..18246)
                     /locus_tag="VM406_10475"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HVL76434.1"
                     /translation="MAKPAKPSTPRSAPPDAAVRQRIEEARAHCASRGAQLTETRALV
                     LELLLRHDGAAKAYDLLAAMQAKSAGVAPMTVYRALDFLVEQDLVHKVESNSSFVVCQ
                     HEHHPHHDPVFMVCQRCGSATECEDPQMSKFLSRALGASGFHAHGVEIKGECGSCSDR
                     QAGR"
     gene            complement(18295..19059)
                     /locus_tag="VM406_10480"
     CDS             complement(18295..19059)
                     /locus_tag="VM406_10480"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HVL76435.1"
                     /translation="MDSLLLEYGWLGMPLLAMLAAAIALVPLGQQVLARGVVFIDLAV
                     AQVAACGALAVAVWIDHPSAWQASIGAGAAALSGAGLVWWLARSWPAQREALIGLVYV
                     AGASVAMVAAAWDPHGRERLTALLAADVLWAAPAPTLWLGTLAVAVAALALARRAALA
                     ADGLFYPLFALTLSAAVPVLGLYLVFAFLIAPALWTRRNVRLGTACALGFVAAVGGLA
                     LSWLLDWPSGACVATALSLAGLASAVRPPGAGTAPC"
     gene            complement(19059..>19241)
                     /locus_tag="VM406_10485"
     CDS             complement(19059..>19241)
                     /locus_tag="VM406_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HVL76436.1"
                     /translation="PRAVVVSSYQDPRPAQWLAQQLGGGVPVLQLPATVEEEGGASEL
                     AGLFDHLIDRLLAQTR"
BASE COUNT         3402 a         6390 c         6360 g         3089 t
ORIGIN      
        1 gcaggatgaa gagcaggaag ctcgcggcga gcggccagcg cttgttcatt tttttgttct
       61 ctccctatca gcgcaccagc tggttgatct cgatgatcgg catcagcacc gccagcacga
      121 tcagcagcac gaccacgccc attacaagga tcagcgccgg ctcgagcagg ccggcgatgg
      181 tcatcgcgcg ccgctcgaga tcctgttcct gggcattgga tgcgcgctcg agcatggccg
      241 gcaattcccc ggttacctcc cctgcgcgga tcatgtggat cagcatcggc ggaaaatgct
      301 gctgcgccga caacgcgcgc gccagcccga cgccttcgcg caccgcattc gtcgcttcct
      361 ccacctggtc gcgcatcgcg acgttggaca aggtgtcgcg gctggtttgc agcgcgcgca
      421 ggataggcac gccggagccg gtggtgatgg ccagcgtgct ggcgaagcga gcggtgttca
      481 ggctgcgttc gaacttgccg tacaacggcg cattgagcag ccagcggtgc cagcgatact
      541 tgatgtcggg gttgcgcagc gccgcacgcc aggcgaagaa ggcggcgacc ataccggtca
      601 gcacgatcca accccaggcg cgcacgaagt cggaaatcgc cagcatgatg acggtcagca
      661 tcggcaggcg ctgcttggtc tgcgcaaaca ccgagacgat ctgcggcacg acataggcaa
      721 gcaggaagat cacgatggca aatgcaacca cggtgacgat ggccggatac gtgaacgcca
      781 ggcggacctt ctgcaccagc gcattgcgcc gctcgatgta gtcggccagg cgagacagca
      841 cgcgtgcaag ctggccaatc tgttcgccgg aggccaccag cgcccgataa atatcgggaa
      901 agtcgcgcgg atggcgcgcc agcgcgtccg acagcgagga gccgcccatc aactccgagc
      961 ggatcgacgc aatcaggtcg cgcatatacg ggcgttcggc ctgctccagc agcgcagtga
     1021 aggcttgctc aagcggcagg ctggcttcca gcaggctggc gagctgacgc gtgaacaggg
     1081 cctgctcgac cgtcgacagg cggtcgccga acgcgcgcgg gcgcgcctgg ccgaactcgt
     1141 cgacctgggc ggcgatcgcg tcgaccgcga tcggcacaag acccttggcg cgcaggtcgg
     1201 aacgggccgc gcgcgcgctg tcggcgttca ggacgccctt gcgtgtggcg ccggcgtcat
     1261 cgacggcttc gtagcggaat gcaggcattg ccgattactc tttcgcgacc cgcagcagtt
     1321 cggcgcgcgt ggtggtgccg tcggcgatcc agcgctcgcc gtcctcgcgc atcgtgcgca
     1381 tcccgttgcg ctgcgcggcc tggcggatgt cggcctcgga cgcctggtcg tggatctggg
     1441 cgcggatttc ctcggtggtc agcaacagtt catagatgcc gacgcggccg tggaagccgg
     1501 tgtgaccgca cttgtcgcag ccgaccgcct gccactcggg accgccgtgc ggatccggct
     1561 gcttgcagtg tgggcacagc ttgcgcacca gccgctgcgc gactacgccc agcagcgacg
     1621 aagacagcag gaagggttcg atgcccatgt cgagcaggcg cgtgactgcg gcggccgaat
     1681 cgttagtgtg cagcgtggcc agcaccaggt ggccggtcag cgaggcctgc accgcgatct
     1741 gcgcggtctc gagatcgcgg atctccccga tcatgatcac gtccgggtcc tggcgcagga
     1801 tcgcgcgcag cgccttcgcg aaggtcatgt cgatgcgcgg gttgacctgg gtctggccta
     1861 tgccctgcag ctcgtattcg atcgggtcct cgacggtgag gatgttggag gtcggcgaat
     1921 tcagccgcga cagcgccgca tacagcgtgg tggtcttgcc cgagccggtc ggcccggtga
     1981 ccagcacgat gccgtgcggc tgggcgatca ggtgatcgaa ctgctgctgc acatccgggc
     2041 tcatgccgag atgggacagg tccaggcggc cgccttcctt gtcgagcaga cgcagcaccg
     2101 cgcgctcgcc gtggccggta ggcagcgtgg agacgcgcac gtcgaccggc ttgccgccga
     2161 cgcgcagcgt gatgcggccg tcctgcggca ggcgtttttc cgcaatgtcg agctgggcca
     2221 tgatcttgat gcgggagatc agcgaggcat gaatcgcctt cttcggccgc accacgtcgc
     2281 gcagcgcgcc gtcgacgcga aagcgcacga ccgatatctg ctcgaacggt tcgatgtgga
     2341 tgtccgacgc ttcctcgcgc agcgcctgcg tgagcagcgc gttgatcatg cgaatcaccg
     2401 gcgcgtcatc ggccgactcc agcaggtcct ccaccgccgg catgtcgagc atcagcttgg
     2461 caaggtcgag gtcggactcg acttcatcga ccacctgggc cgcgtcgccg ccggagttcg
     2521 cgtaggcgcg cgcaatcgcc gcctccagtt cctcgcgcga cagcgacttg agcttgacgc
     2581 ggccgaaccg gcggctcact tcagcgatgg cggaaggggc gctcccctcc gagacccaca
     2641 gctcgacggc atttccgggc tgctcggcgc gacgcgcgag cagcaggtgg tcgcgcgcaa
     2701 atgcaaatgg aagaagacgg gcgtcggtga cagcactcat tgggtttgct tcggtcgtaa
     2761 atcaatttcc ggcacctggc tgcctggagg cagcggggtc ggggcaggca gcggctgcag
     2821 cggttgtgtc ggaaccggct gcggctcggc gccttccggc aacgggcgcg gcggcggcag
     2881 caccagcggc gccgcgttca cctggccgga ctcggaaggc ggaaggatgg gcgcatcgat
     2941 ctgcggcagg ccgaacgcag gtgccggctg acgcaggatc tgcatgttgc ggatgtagtc
     3001 atagcggtcg ccggacacat gcacactctg ttcgttggtg cggatcacgg tcgggcgcag
     3061 gaacaccatc agattggtct tggtgccgcg gcggctctgg tacctgaaca ggttgcccag
     3121 caccggcacg tcgcccagac cgggcacctt ctcgacgttg tcggtcacgc ggtcttcgat
     3181 caggccgccc agcacgatga tttcgccgtc atcgaccagc acgttcgttt caatcgaacg
     3241 cttgttggtg atgatgtcgg ccgcgttggt ggtcggctgc aggttagagg tttcctggta
     3301 gatctgcatg cgtacggtgc cgccctcgga cacctgcggg cgaacccgca atgacaggcc
     3361 gacatcctgg cgctcgatgg tctggaacgg gttgacgttc gcgccgcccg cctgggtggt
     3421 gaactggccg gtgacgaacg gaacgttctg gccgaccaca atccgggcct cttcgttgtc
     3481 gagcgtaatc aggttcggca tcgagagaat gttcgcgttg ccttcggact ccagcgcccg
     3541 ggccaacgca cccagggtca gttgaccgtt gatttgccgg aaaattccca ggttgatgcc
     3601 gttcccgggc ggaagcgggt tgccggtcgc ggcgcgcgag accacttggt tgacgaggtt
     3661 gttgccggcc tggccaaatc cgctgatcac accgacccgg taatcgctgt tgttcgtgcc
     3721 ggacaggccg gcccattgca cgcccagctc ggcggccctg tcggcggtga gctcgacaat
     3781 cagtgactcc acgtagacct gcacccggcg cgcatcgagc tggtcgatca ccgcgcgcag
     3841 gttacggtag acggactcgc tggcggtgat gatcagggaa ttggtggccg ggtcggcctg
     3901 gatgaagcct gccggcccgc cggcaggcaa cgcggtcggc gtggtctgct gcggcagttg
     3961 cccctggccc agcgccgcct gttgctgctg cggctgttgc tgctgctgcg ggggttgttg
     4021 ctgctgggcg gcaccgggct gggcgcccgt ctcgcccgcg accaccgcgc gcagggtctg
     4081 cgccaggcgc accgcgtcgg cgttcttcag gtagaccacg tgcacgttgc cgggctgggc
     4141 cgtcggctgg tccagccgtg caaccagcga acgcgcgaga ttggcgcgcg cggccgaagg
     4201 cgcccgcacg atcaccgagt tggtgcgggg atcggccagc agcgtcatgc gcccggcctc
     4261 gccgccggcg gccggcgccg cgccctggtc gagcaggcgg ttgaccatct gcgcgatgtc
     4321 ggtggcgatc gcatgctgga tcggaatcac gtccaggtcg ccgaccgccg gcgcatccag
     4381 ggtggcgatg atgcgaccga ggcgacgcag attgtcggcg taatcggtga tgacgaggct
     4441 gttgttgccg ggattgacgt tgatcgtatt gttcggcgag atcagcgggc gcaggatctg
     4501 caccagattg ttcgcgcttt cgtggttcag gcgaaatatc tgggtggcga tctggtcgcc
     4561 gcgcacttcg ggtatctgaa cgggcgctgc ctgcagcttc gcatcggctt cgggaacgat
     4621 cttggtgaag ccgtccgtgc gcaccatcgc aaagccttgc agacgcagcg tggatgccag
     4681 cagctgaagg gcctgggcgc gcgtgaccgg tcgttccgat accagcgtga tcgtcccctt
     4741 gacgcgcggg tcgatgatga aggtggtgcg ggtgtaatgc ccgaccgcct tgatgacgga
     4801 ttcaatatcg gcgccgacga aattcagcgt tgcttcgctg gcggtcggct cctgggtgag
     4861 ggccggcggc gtgacagtgc ccaatgcgca cagcaaggcg gctgcaacgc cgatgcggcg
     4921 aaaaccgatg acactccggg gaaatgctac agctgatcga ttttttttca tttgaactct
     4981 agcgcgataa catccttgcc gttttctcga cggcgttgtc ccagcaaatt caacagattt
     5041 gccagcctgt cttcctgccc tgcctccgct tcggcctttc cggaaaattg caatcgtcca
     5101 ttgatcaggg tgccgttgcc actcaataac atcggccctt tgattgtggt caacgccagc
     5161 tgcgcctgct gcccgcgcca gtccatcttc atgcggtagg caccaagcgg ccgcaccggc
     5221 gacaggcgcg acgaaacgtt ctccagctgc aaagtcatgg tcccgtggag atccacggtg
     5281 ttcgtccggc gggtcatctg cagcggcgtc cagctcagct gcatcgtgcc tgaaggctgg
     5341 atcgtgttca gcggcgcacc cagtcctgcc aggcgttccg ccggcagcat gacgtgcgcg
     5401 gggctgacct gccagtccca ccagctgcca gaaatcgaca ccggctgcga cagcgagtcc
     5461 ggattgcgca gttcggcgtc gacctggcca agcagcacca gcggcgacag ccgccattcg
     5521 aaacgccccg gtagcagcgg cgtgaccgga tcgttgccgc tcggcgcacc gccgatgaaa
     5581 gccgagccgc gccagatcga gccttgcgcg tcaccgagtg tcagacgccc gccggtctgc
     5641 gcctcgacgg ccggcgccag ccaggccgcg gggaagaaca cgatgaccgt gagcgcgatg
     5701 ctcaacgcgc cggccagcaa ccatatcacg aggcgtcgca tgcccggcct cttttacttt
     5761 gcatcaactt cattgcgcct gcccgcgttc ctggcgcaat gtgtaggcag cattcacgct
     5821 gtcgggctcg cccccggctt cgaccttggc ctcgaccacc gccagccgtg ccgtgcggtg
     5881 catctcggcc agccattcgg tctgcgcggc gaacgaggca ccttcgaaac gcagccgcac
     5941 cagttcaccg ctgacactga tttcctgcgc ctgcaggccg cggcgcgaca gcgaggtctg
     6001 cagggtctcg cgcgtgaccg ccggcgccgt cgcggcggtg cgcgaaccgg cggcggcagc
     6061 ttcgcgcgtc agcgcctgca cctcggccgc ctgctggcgc agggcaggca gccgctgggc
     6121 aagctcctcg cgcccgctga gggccggatc gagcagcaag aggtagatga gagcgaggac
     6181 cagcacgccg gccgccgcgg caagcatcgt gcgttcgcgc gcattgcgct cgttccagta
     6241 tgcgacggcc gactggcgca tcgcctgcaa cctgttcatc gtgtgctcct gatctgcagg
     6301 acgccttcgc cttgctcgga tacgtccaga ttgcgcgagg ccagcgccgt gttgagggct
     6361 tcgggatcga cgcctgagcc gggtttcatc cgtactgtca gcgtacgctc acggtagtcg
     6421 agcgaagcca ccggcgcgcc gccggtgccg ccgatcgcct gccaggcctc ggccagcccg
     6481 gcgctcagcg ccaggaaatc gccggctgcc agttcgccgc tcgcattgcg cgccgccgcg
     6541 gccttctggc gcgcctgcgc gagcggatcg acaatgacag tgtcttgcgg aaaggcgctg
     6601 cggtagttct gcagcatgct cgcacgcagc gtctcggcct cgcgctgcat gcgcatccag
     6661 tccacgttca acccgatcag gttgaccagt accaggccgg cggccagcgc taccggccag
     6721 cgcaggcggc gccagtcgat cttcgggccg gacgcaccgg acaaaccgct gatcaagtcc
     6781 aggccggcct gccgggcgcc ggcaatccat atcgaccagt tgtcttcttc gagcgtgatt
     6841 cgctgctcga gcgcgggcac caggtgcagc gagccttgat agtcgtgcag cttggcgggc
     6901 ggaacgtaga ggacaaccgg cgcttgcggc accagcgcgt tgagcgactg gatgacttcg
     6961 aacggggccg attcgggcgg atcggccagg acggagacgc ccatgccttc ctgcgccccc
     7021 atgcgcaccg tcagatcgat gtcgatgccg tgttcggcca ccgccgcagc cgccgcttcg
     7081 ccggcgatcg gcagacacaa ctgcgcaggc accgcgctga gcttgcgcgc tcccagcgtg
     7141 gtgagggtac gcgacaggat ctccagccag ctgcgctgga ccacgccgac cgtgcgcagg
     7201 ccatccttca gctccccggc gacgaccacg cactctgccg gatcgctcat caggtggtct
     7261 tcgaccaggt tcggcagcgc cgccttcagg cgtgcgccgg tcagcggcgg cattttgatg
     7321 cgcagcagcg tcacgtcgga ggcggccagc agcagcacga cctggcgcac gcgcttgacg
     7381 gcgtcgccga gctcggacag tgcggcaatg ccctcgcgct cgatgcggtc cgcatcggac
     7441 gcgatcgcgt atgcgcagta cagcggcatg cccggctgca gggacccggc tcccgcgtgc
     7501 gcgggcagcc gtatgaaaag tgtgctcaag aggtttgcct ccctagtatt cgcgtatcca
     7561 gatcagcgac ggctcggccc gacccggccg gcggatcagc gcctgcatgt ccatcgcggc
     7621 gcggctcatg cgcacatggc cgttgatcag gaagtattcg gtgcgcacgt cgatgtgctg
     7681 cactgctgcg cctcccatcc ccatctggcg caaccggtcg acaaactcgt tggtcgagcg
     7741 gaaccaggcg ttctcgcgcg cggcaacgag agccgcggca tcggacatgg aaagcctttc
     7801 aattctcgca gacaacactt ccgccggggc agtgttgaca ttgatcgcgc gcaggtccgg
     7861 cagcacgacc acgaattcgg acagccgcgc gacgatgtcg ggcgtgaagc cgggcgcggc
     7921 aagcaggtcg tcgacatgtt cgacacgcat ctgggcgccg cccgcggctt gctgaccgcc
     7981 cgcggcgccc tgcccgcccg gcgcgcgctg gccggctgcg accaagtcgg ccgcagcctg
     8041 cgccaatccc gcgttcagtc ccagattgac cagcaggcgg cggaacgcgg ccagttcctg
     8101 cggattgatc gtgccgttgg ccgccaggtt gttcagattg aagcgcgact gtgcgtcgac
     8161 gatgctgccg gagagcaccg cgtcggacgc ctccggattg gcgcgcggat tgtcgacgta
     8221 ctcatccagg cgcgtctcgg ccagcggcac cgcccagggc tcgcccaggt gatcctccgc
     8281 cgtcattgcg ccgtccgcac gcaggatcag ctgcgcccag tcgagcgcgc cgcgcaggat
     8341 ccattgcttc tgcaattgca tgcgctgatt ctcgatcgag cgcacctgca cctgctgctg
     8401 ccagaacagg ctgccgacga tagtcacggc cagcgtggtc agcaacagag ccgtcacgat
     8461 cgccacaccc ctctgactct gtctgctgcg cagtctcatg cattccccag catgaagatc
     8521 ttgaacatgg tgccggggcg ctcgaccagg tccaggccca cttccacccc gatcagggga
     8581 gcattgcccg gcgccggcgt accgctttgc cgccagccca cgttctggta ccacagccgc
     8641 agattcatgt tctgcaccga gcggtgcaga accaccggcg gcgtcggatc ggcatcgccg
     8701 agcgcggcgg tccaggcggc cgcgagcagg ttcacatcgc gcgtgggcag cgactcgcgc
     8761 cgcgacagta cgccgtcctg caggcggtat gacaccacct gcacgcggga aggctggttt
     8821 tccgcgtaga ccatgcgcac cagggtcagg cggccattgc cggcgtcgac gaccggccgg
     8881 ttgccgatcg cctgcggcag cgcgatcttg tcgcaatcgt tctgcatctg cgcgaatgtc
     8941 agctgcagcc cgcgcgtctg ttccaggtct cggttgagcc cttcgcgcgc acggatgatc
     9001 gtgtccagcc cgcgccagcc catcacggcg acgattgcca acaccgagat ggcgaccagc
     9061 agttcgatca gcgtaaagcc gcgcgcgcgg cgaagccgga tgttcattgg cgcctcggca
     9121 cgatctgcgt gagccggatg atgcggcgct ccggatcctc tgcactggaa acgctcacct
     9181 cgacgcgccg gaaaaatgga ttgggggtgg ccaccacctc ttcctggcac accagctgca
     9241 gttcggcctg cggacaaggg aagctgcgcc ggcccagcgg cgggaactcg cgcgacaggc
     9301 gcagctgggt caagcggttc tccgccgacc aggtagccat catcgacgcg cgcagcgcgc
     9361 tgccgttttg catcaggctg ccgaccgcgc gcagcgaggc tccgagcgcg gtgccgacga
     9421 ccaccatcgc caccagcact tcgagcaggg tgaaaccgcg tgagcgtcgg agagaggagc
     9481 ggcgcggcat gtcattcaac cgtgaaattg ccgatcccgt cggcacggat cgcgacgctt
     9541 gtatcgccgc gcgaaagcat cagcacgaac ggccggtcga ccggttcgcg cccgaacaca
     9601 agacgcacct ggacttcgct cgcaccagcg gcgttgaccg cggcgggcga ggtggtgagc
     9661 gtgacgggag cgagcgcaaa cggtcgttcg cgcagcagat cgtcctgcgg aaccgcaatc
     9721 caggcattgc cctcgcgttc aagaaaacgg tagcgagccg aatcggcctc gaacgcgatc
     9781 gcacggttgc gcacgattgc ctcatctcgc gccagttgca gaagcagtgc gatgcgctgg
     9841 gcctcggtct gcagcgcttg gcgctcgccg ggcattgcat tgaaggcgac cacaccgagc
     9901 aagagtccgg cgatcaccat cacgaccagg atttcgagca gggtgaagcc ctgctcgcgc
     9961 gccgggtgcg gcgcccgtgc gcgccattca ggaacggaac aggaaggcgt tacagatccc
    10021 acgaaccgat gtcggcgtca ttgccgttgc caccaggctg gccgtccgcc ccgtaggaga
    10081 acacgtcgaa ctcgccgcgc acgcccggcg acaggtattg gtaagaattg ccccaggggt
    10141 cgcgcgggag cttgtcgata tacccgccgc tcttccagcc attggccgcc gggccggtgg
    10201 tcggacgctg gaccagcgcc tgcaaaccct gttcggtggt cgggtagcga tggttgtcga
    10261 gcttgtagag cttcagcgcc gagctcaggg tggcgatgtc ctggcgcgcg gccgtgatgc
    10321 gtgcgtcgtc cgcacgtccc atcaccttgg gcatgacgac cgccgccagc acgccgatga
    10381 tcaccacgac caccatgatt tcgatcaggg tgaagccgcg ctgcagggtg cgcgaaagcg
    10441 gacgtcgaat gggggaaaaa gctgtttgca tataactact ctctggctaa gggacgacag
    10501 aaggggggag tataagccga agccggccgt ggcgagcgct gctcgccctc cctctcgcaa
    10561 ggtttcctgt gtgactgggc ggtggcccaa aagtgccccg tggaatgatc cctcagcgaa
    10621 aaaaaacggc cttgcggtaa cgcatatcgt gtcgttgggc aaagatctgc gcggcctcat
    10681 ggtggaacaa acatgccgga ccaacactct tgaagatgac gcttgcaata aggagaacag
    10741 catgtccctt ccccatgccg catcgggcga catcatcgat gtccggccgc tcggtccaaa
    10801 gcttgccgat gcggtatcga ccgcactgtt caagaccgat cagctcgaag cgatgcggct
    10861 ggtgttgacg acaggcaagg aaattcccga gcaccatgtc gccggcgact tcactttgca
    10921 ctgcatagag ggcaaggtcg aattgcgcgt caatgccgac gtgcgcaccc tgcaggcggg
    10981 cgagatggtg ttcgtcgccg ccgaggcgcc ctattcgatc cgggcgctgg ccgacgcttc
    11041 gctgctgatg acgctcgcgc gttgccgcga aggtggttga aggcgctcag gtcgtttctt
    11101 cgcgcgggtc gcgggccagc agccgctgca ccatcgcggc cggcgagtcg ccctcgaaca
    11161 gcacggcggc aaccgcattc gtgattggca tgtccacctg atgcgcggcg gccagagcac
    11221 ataccgcctg cgcgcagcgc acgccttcgg ccacgtggcc aagttcggcc accaccgcgt
    11281 ccagcttcaa tccccgcgcc aatccgaggc cgacacgccg gttgcgcgac aggtctccgg
    11341 tgcaggtgag gatcaagtcg ccaacgccgg tgagccccat gaaggtttcg ctgcgtccgc
    11401 caagcgcgac accgaggcgg gtgatttcgg caagtccgcg ggtgatcagc gcggcgcggg
    11461 cattgagccc cagaccgagg ccgtcgacca cgccggtcgc aatggccagg atgttcttca
    11521 ccgctccgcc gacttccacg ccgaccaaat catcgctgct gtagacgcgc agattgccgc
    11581 catgcaccgt ttcgaccacg attgtgcgca gcgccgccga ggaagacgcg accgtcagcg
    11641 cgcacggcag gccctgcgcc acctcctgcg cgaacgaagg tcctgagagc gcgccgcagg
    11701 gcagcgcgtc gcccagcacc tgggcaacga cctggtgcgg aagcagttgc gtatgctcct
    11761 caaagccctt gcacagccag accaggttct ggatcggatg gcttttcagc cgctcggcga
    11821 gcggacgcag gccggccacc gaactggcga cgatcagcag accgtcgccg gttgccgcat
    11881 gggcgacggc cgcatcgaaa tcggcggtcg gcctcaatgc cgatggcaac gcaaaaccgg
    11941 gaagataggc gctgttctcg tgctgcgcgg cggcctcgcg catcgccgca gcgtcgcgcc
    12001 cccacaggat gacttggtga cgccgcgcga gcacgatgcc cagcgcagta ccccacgcac
    12061 cggcgccgag gatggaaatg ttccgggacc ggttggtgcc ggcggcaaga acggacatcg
    12121 cgctattcgc cccagacttc gcccgcgcgc acgaaaccgc cctggccatc ggcgtgccgt
    12181 acgcgcaccc agccggcgtt gaccggctcc agcatgtcca tcagcacgcc ctggctcgcg
    12241 ctgaacacga ccggagatgc ctcgtcgggc gcctggtgca cgcgtgccgc ggcggcattc
    12301 accacgaccg tgcggcgagc ggccagtgcg cgcgtctcga cccatgcgag atcgccggcg
    12361 gcgtcgcgca ccttggtcca ttcgccatag gtcagcacga cttcgaccgg catgccgcgt
    12421 ggtgctacga acacccgccg ccccttggcc gacggcgcgt cgtacaggat cgccggcgac
    12481 gaaccaaccg aacggtattc cagcgcgtgc gcgctcccgg ccgccgcgac gagcgcggcg
    12541 gcgatcagcg caagccgtga cagtctcatg cgcgctgccg ggaagacgcc tcgcgcgctc
    12601 actgcggacg agccgccgtg ccgtcgggca tcacgatgcc cgcgccctgg ccctgctcct
    12661 gctgctgttg cgccagtgcc tgcagacgct gctgatagaa cgcttcgaag ttgatttccg
    12721 caaggtgaac cggcggaaag ccggcccggg tgatcacgtc ggcaatgttc gcgcgcaggt
    12781 acgggtagat gatgttcggg cagccgatgc ccagcagcgg atcgagctgc tccggcggaa
    12841 tgttggccgc ctcgaagatc cccgcctgct tgccttcgac gaggaaggcg accttgtcct
    12901 tgatcttcgc agtcagcgtg atcgtgaccg tcgattcgaa gatcgtttca gccagggcct
    12961 cgacaccgac atcgaccgcg acttcaatgt tcggcgcttc ctgctcgagg aagattgccg
    13021 gcgagttcgg ctgttccagc gacatgtcct tcaggtagat gcgctggatc tggaatacgg
    13081 gctgctgatt ctgttcggcc atgatgttct ttctgtggat acgtaggagg agggatgaga
    13141 agggatggcc gcggccatcc cggaagaact gcgcgacgaa ccgaagccgt ccgctgcgct
    13201 cagccgccgt tgagcagcgg atcgagtttg cccgcgcggt ccagcgcgga caggtcgtcg
    13261 aagccgccga catgggtttc gccgatgtag atctgcggta ccgtgcgccg gccggtcttc
    13321 tgcatcatga cggcgcgctg ctgcggatcg aggtcgatgc ggatcttctc gatctccttg
    13381 acgcccttgg acgtcagcag ccgctcggcc atgacgcaat agggacagac ggccgtgctg
    13441 tacatcaaga cgcgtgcggt cataggtgct ccgttatttt gagacaggca ggccggcggc
    13501 ttgccatgca ttcaggccgc cttcgaggct ataggcctgc tcgaaccccg ccttgcgcag
    13561 gattgctgca gccttggccg actgggcgcc cgactggcac acggcgatca ccggcttgct
    13621 cttgtgtttg gacaaatccc cgagccggtt cgacatttcc tttagcggga tgttctttgc
    13681 atcacgcagg tggccggccg catacgcgga aggttcgcgc acgtcgatga cgaccgcgtt
    13741 ggactggttg atcagctgcg tcgcgcgcag caccgagaca ttgacgcccc gtcccttcag
    13801 acttggccac agcagcaggc cgccggaaat gacggcgacc aggatcagcc atatgttatc
    13861 gagcaagaaa tccacagcat tcaaatggtt ggaagaaacg cgccattata aaatagatgc
    13921 cggcaagcga ccccttttgc ggtccgcact ccctcttcca actcactccc cctcacaaca
    13981 tgtacaagat cgtattgatg cgccacggcg agtccacctg gaatctagaa aaccgcttca
    14041 ccggctggac cgacgtcgac ctcaccgaga aaggcatggc cgaagcgcgc caggccggcc
    14101 gtgtcctgaa ggaagccggt ttcacgttcg acctgtgcta cacctcggta ttgaagcgcg
    14161 cgatccgcac gctctggctc acgctcgacg aaatggacct gatgtggttg ccggtggtgc
    14221 acgactggcg cctgaacgaa cgccactacg gcgcgctgca ggggctgaac aaggccgaga
    14281 ccgcagcgaa atacggcgac gagcaggtcc tggtctggcg ccgcagctac gacgtgccgc
    14341 cgatgccgct agacccggac gacgaacgca cctcctacaa tgacccgcgt tacgccggcc
    14401 tgtcgcgcga gcagattccg ctcaccgagt gcctgaagga cacggtggcg cgcgtgatgc
    14461 cggcctggca cgactcgatc gccccggcga tccgcgcggg tcggcagatc ctgatctccg
    14521 cccacggcaa cagcctgcgc gcactgatca aggacctcga cggcatcaac gacgccgata
    14581 tcgtcggcct gaacattccg aacggccagc cgctggtata cgaactggac gccaatctca
    14641 agccgatcaa gagctactac ctgggcgacc aggaagccat cgccgccgcg atggccgcgg
    14701 tcgccagcca gggcaaggca aagtaagcgt tgatgcggat tctctccgga gccggccggc
    14761 gcgggaccgc gcttgccgcg gtcggccttg ccggcctgct gttctgcctg tccgcccatg
    14821 cgaacaaggt caccgagcgc agcaagcagc gccaggcggc cgaggccgag cgcgccgagc
    14881 tgcgccagaa gctcgcggca ctgaagcgcg agatcggcca gaccgagacc gcgcgcaccc
    14941 atgccgccga cgcactggcc gactcggaag cggcaatctc gcaggcgaac cgcgcgctgc
    15001 gcgaactggc acaggagcgg cgcgacgtcg aggcgcggct ggccgccctc gcgcgcgacg
    15061 aaaagcgcct gaaccaggcg gtggccgagc agcgcgcgcg cctgtcgacc gtgcttcgcg
    15121 agcagtacat gggcggcagc gacgaccgct tcaagctgct gctgtccgga gacaacccga
    15181 accgcatcaa tcgcgacctg cagtacatgg gctatgtgtc tcaggcccag gcgcggctgc
    15241 tcgcctcgct gcagcaggat ctgcaggcga tcgaagccaa ccgcgcgcag gcgcagagcg
    15301 cgcgcgacga ccttgacgaa atcgcacagg agcagcaggc cagcagggcc aggctcgaac
    15361 aggaaaaggc gcgccgcgct gcgctgctgg ccgagctgtc gagcaggctc gccgcccagc
    15421 gcaaggaggc cggcaacatc gaacgcgacc agcagcgcct gtccgcgctg gtcgaccgcc
    15481 ttgccaggct gatcgaggaa cagcgcaagg ccgaggaagc cgcgcgccgc aagcaggaag
    15541 agcagcgccg ccaggaacag ctcgcgcgcg cgcgccaggc cgaggccgcg cggcaggcgg
    15601 aagccgcaaa ggcgagttcg ctcaataccg cgtcccgcaa acctgatgca atcgataacg
    15661 cgccggcaaa agttctgggc aggaatgaac tcacgccgga cgcccgcgtc caagccgcgg
    15721 tgccgggtcg ggcattcggt tcgttgcggg ggcagttgag gctgccggtg cgcggcgaat
    15781 tgacagcacg ttacggcagc acgagaaccg atggaccgag ctggcgcggg ctgttcatcc
    15841 gcactgccga aggcgaggaa gtcaaggcag tggcggcggg acaggtggtc ttcgcggaat
    15901 ggttgcgtgg ctttggcaac ctgatcatca tcgatcacgg cggccagtac atgaccatct
    15961 acggaaacaa ccaggccgta ttgaagcagg tcggcgacac cgtcaacacc ggtgacgtca
    16021 tcgccaccgc cggcagcagc ggcggcagcg aacattcggg tttatacttt gaaatgcggc
    16081 atcaaggccg cacctttgac cccctcggct ggattgcgcc cggcacgctc aagtagtcgt
    16141 gccggtgccg aatgcggcgt ggacgggcaa accatcaggt gacaaatggg cagcaaactc
    16201 aagaacttcg gccttatcgg tctgggcatc atcgccggcg tcgccggctc gatgcaattc
    16261 gaggccagcg cgcagaagaa cctctcaccc ctgccgctcg aagagctgcg ccagctggct
    16321 gacgtgttcg gcctgatcaa gaccgactac gtcgagccgg tcgaggacag gaagctcctc
    16381 accgaagcca tctcgggcat ggtcgcctcg ctggacccgc actcgtccta tctcgacaag
    16441 aaggccttca aggaactgcg tgaaggcacc cagggcaagt tcgtcggcct gggcatcgag
    16501 gtcggcatgg aagacggcta cgtgaaggtc atctcgccga tcgaggactc gcccgcgttc
    16561 aaggccgggc tgaaggccgg cgacctgatc acccggctgg actccacccc ggtcaagggc
    16621 atgacgctgg acgaagcggt gcggcgcatg cgcggcgagc cgaacaccaa gatcacgctg
    16681 accatcgccc gcaaggaaga ggaccgcccg atcatcgtca ccatcgtgcg ccaggaaatc
    16741 cgcgtgcaaa gcgtgaagtc gaaggtcgtc gagccgggtt acgcctgggt gcgcatcgcg
    16801 cagttccagg agccgaccgt ggacgacatg gtcaagaagc tgatcgacat ccacgccaag
    16861 gaaccgaacc tgaagggcct ggtgctcgac ctgcgcaacg atccgggcgg cgttctgccg
    16921 ggcgcgatcg gcgtgtcggc agccttcctg ccgaaggatg tgccgatcgt ctccaccaac
    16981 ggccagcttg ccgattccaa gcagacgttc tacgcgcggc gcgagttcta cacctcgtcg
    17041 cgcctgtcgg tcggcgaccc gctggcgaag ctgccggcga tctacaagga catcccgatg
    17101 gtcgtgctgg tcaataccgg ctccgcctcg gcctccgaga tcgtcgccgg cgcgctgcag
    17161 gattacaagc gcgcgaccat catgggcacg cagaccttcg gcaagggctc ggtgcagacg
    17221 atccgccagc tgtctcccga caccgcggtg aagctgacca cggcgcgcta ctacacgccg
    17281 aagggccgcg cgatccaggc caagggcatc gtgcccgacc tgctggtcga tgaaacgccc
    17341 gagggcgacg ggctgaacgg cctgcgcctg cgcgagtcgg atctgcagaa gcacctgtcg
    17401 aatgaccgcg acaaggaaca gatcaaggcg cgcatcgatg aactcgaaga gcagcagcgc
    17461 atcgccgcgc tggagaagcg ccgcaagccg ctggaattcg gcagcaccga ggacttccag
    17521 ctcgcgcagg cgctgaacca cctgaagggt ctgccggtgc agctgtcgaa gacggcgacg
    17581 atcgaaagca agaaagagcc gacgatcaac gatccgcatg ccggcgagaa gaacggcaac
    17641 ggagcggaat cgaagaagtg atttgatttc cgggaagaga aaaggccgca gcgatgcggc
    17701 cttttttgtt gcaagaacgg ccgcggcaag cgaccgatcg gcttgtgcca gctagcgccc
    17761 tgcctgccgg tcggaacagc tgccgcactc gcccttgatc tccacgccat gcgcgtgaaa
    17821 gcccgacgca cccagcgcgc gggacaggaa cttgctcatc tgcgggtctt cgcattcggt
    17881 cgccgagcca cagcgctggc agaccataaa caccggatcg tgatgcgggt gatgctcgtg
    17941 ctggcagacg acgaaggagc tgttggattc gaccttgtgt accaggtcct gctcgacgag
    18001 gaaatcgagc gcacggtaga cggtcatcgg cgccacccca gccgattttg cctgcattgc
    18061 cgcgagcaga tcataggcct tggccgcacc gtcatgacgg agcagcaact ccagcaccag
    18121 cgcccgcgtc tcggtcagtt gcgcgccacg tgaagcgcag tgcgcgcgcg cctcctcgat
    18181 gcgctggcgc acggcggcat cgggtggagc ggaccgggga gtggaaggtt tggctggctt
    18241 ggccatcgaa ggtgctgctg atgcaatgga gacagacgcg gtgcgtgggc cattctagca
    18301 tggggccgtc cccgctcccg gcggccgcac ggccgatgcc aggcctgcaa ggctgagggc
    18361 ggtcgcaaca caggcgccgc ttggccagtc gagcaaccac gacagggcaa ggccgccgac
    18421 ggctgcaaca aagccgagcg cgcatgctgt cccgagccgt acattgcggc gcgtccacag
    18481 cgccggcgca atcaggaagg cgaacaccag gtacaggccg agcaccggga ccgccgcgct
    18541 caacgtgagc gcgaacagcg ggtagaacag gccgtcggct gccagtgcgg cccgccttgc
    18601 caacgcgagc gccgctaccg ccacagcgag cgtgccgagc cagagcgtgg gcgctggtgc
    18661 cgcccacagc acatccgccg ccaagagcgc tgtcaggcgc tccctgccat gcggatccca
    18721 ggcggcggcc accatcgcga cacttgcccc agccacatag acgaggccga tcagcgcttc
    18781 gcgctgggcc ggccagctgc gcgcgagcca ccataccagc cccgcgccgg acaaggcggc
    18841 ggccccggct ccaatactgg cctgccaggc ggacggatgg tcgatccaga ccgcaacagc
    18901 cagcgcaccg caggcggcga cctgggctac cgccaggtcg atgaagacca cgccgcgtgc
    18961 aagtacttgc tggcccagcg gcacgagtgc aatcgccgct gccagcatgg ccagcaacgg
    19021 catcccgagc cagccgtatt cgagcagcag actgtccatc agcgtgtctg cgcgagcagg
    19081 cgatcgatca ggtgatcgaa cagaccggcc agttcggacg caccgccctc ctcctccacc
    19141 gtcgccggca gttgcagcac ggggacgccc ccgccaagct gctgcgccag ccactgtgcc
    19201 ggccgcgggt cctggtaact ggagaccacg accgcccgcg g
//
LOCUS       DATLGF010000343         4784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_102863, whole genome shotgun sequence.
ACCESSION   DATLGF010000343 DATLGF010000000
VERSION     DATLGF010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102863"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..1904
                     /gene="uvrC"
                     /locus_tag="VM406_10490"
     CDS             48..1904
                     /gene="uvrC"
                     /locus_tag="VM406_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HVL76437.1"
                     /translation="MSDQVLPEPRAALLDTVARLPNLPGVYRYFDTDDRLLYVGKARD
                     LKKRVSSYFQKSLASPRTAMMVARIARLETTVTRSEAEALLLENNLIKTLQPRYNILF
                     RDDKSYPYLKITNQDVPRMAYYRGGVDKRHQYFGPFPSAWAVKESIQILQKVFQLRTC
                     EDTVFANRTRPCLLHQIQRCSAPCVNAISREDYALDVENAAKFLRGRQGEVMESLEQK
                     MHAYAAELKFEQAAAVRNQISALSRVLHQQSMETVGDADIDIIAAIVQGGRACVNLAM
                     VRGGRHLGDRAYFPTHVEDAVAAAEDSVELEVLKAFLAQHYIDKFIPSTLILNLEMDE
                     PELMTALMEQCGHRINLVFQPQGQRRQWLEMAQKGAEIALARLLSEQGSQQARTRALA
                     EVLGLETDDLDALRIECFDVSHTQGEATQASCVVFHHHAMQNGEYRRFNITGVAPGDD
                     YAAMRQVLSRRYEKVAGGEGVLPDIVLVDGGKGQVEIARQVFSEFGLDTGIIVGVAKG
                     EGRRVGLETLIFADGRVPLELGKESAALMLIAQIRDEAHRFAITGMRAKRAKARQASR
                     LEEIEGIGSKRRQRLLARFGGVRGVMDASIEELASVEGISRQLAEQIYRQLH"
     gene            2005..2586
                     /gene="pgsA"
                     /locus_tag="VM406_10495"
     CDS             2005..2586
                     /gene="pgsA"
                     /locus_tag="VM406_10495"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HVL76438.1"
                     /translation="MPFNIPILLTWLRVALIPLVAGVFYLPETFLSREMQGLASALIF
                     IVAAVTDWLDGYLARRWNETSAFGAFLDPVADKLMVAGALLVLVELQAVNAVVAFIII
                     GREIAISALREWMAQIGASKSVAVSSLGKIKTAAQMIAIPMLLYSGIVAGIDLRHLGA
                     WLLVIAAVLTVWSMFYYLRKAWPLIKEKAARAH"
     gene            2656..2731
                     /locus_tag="VM406_10500"
     tRNA            2656..2731
                     /locus_tag="VM406_10500"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2689..2691,aa:Gly,seq:gcc)
     gene            2818..2893
                     /locus_tag="VM406_10505"
     tRNA            2818..2893
                     /locus_tag="VM406_10505"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2851..2853,aa:Gly,seq:gcc)
     gene            2952..3025
                     /locus_tag="VM406_10510"
     tRNA            2952..3025
                     /locus_tag="VM406_10510"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2984..2986,aa:Cys,seq:gca)
     gene            3076..3165
                     /locus_tag="VM406_10515"
     tRNA            3076..3165
                     /locus_tag="VM406_10515"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3110..3112,aa:Leu,seq:taa)
     gene            3213..3683
                     /locus_tag="VM406_10520"
     CDS             3213..3683
                     /locus_tag="VM406_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76439.1"
                     /translation="MPQLSAPTLAPAQILMRMAAPSKHRGVLWISKAASIGASMANFP
                     LSRRRRWWGGAMFAAMLVAPGITVADDVGSASSSQQQQIDDPRTQLQLLQSQTQYQEM
                     QARHEEVRQRMEMEARMRQQETQQHVQQRIREQRMEMQMHAHEQRRQLEQERRR"
     gene            3909..4142
                     /locus_tag="VM406_10525"
     CDS             3909..4142
                     /locus_tag="VM406_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008345654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3072 domain-containing protein"
                     /protein_id="HVL76440.1"
                     /translation="MDKHTQEQAEHSNMVKDPKDWTTGDEPMTGAQKSYLKTLSEEAG
                     VPMDENLSKAEASQRIDELQQQTGRGMTGMHRH"
     gene            complement(4167..>4784)
                     /locus_tag="VM406_10530"
     CDS             complement(4167..>4784)
                     /locus_tag="VM406_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76441.1"
                     /translation="GAADAGVLLGTTGGGLHVSVPVHHKLSVRTSVNALPDLREERSA
                     GTLAYTSRRNIRTAELLLDWHPWGNMFRVSTGLVINGNHVAAVARPDPDLSYRIGGRE
                     YDVLEVGEVRGRVDFNRLAPFIGVGLTTPPSQYGGWSVAVDIGVFYQGHARSARLWAP
                     DCRVEVPGLCEQLNVDLLDEVPRLRGELPDLRFFPVIRAGLFYRF"
BASE COUNT          911 a         1426 c         1568 g          879 t
ORIGIN      
        1 gagcggcgaa gaagaccgtg caggccctga tggaggcgta agccgcaatg tccgaccagg
       61 tgcttccgga gccgcgcgcg gccttgctgg acacggtggc gcggctgccg aatcttcccg
      121 gcgtctaccg ctatttcgac accgacgaca gattgctgta cgtgggcaag gcgcgcgacc
      181 tgaagaagcg cgtctccagc tattttcaga agagcctcgc cagcccgcgc acggcaatga
      241 tggtggcgcg catcgcgcgg ctcgagacga ctgtcacgcg cagcgaggcg gaggcgctgc
      301 tgctcgagaa caatctgatc aagacgctgc agccgcgcta caacatcctg ttccgcgacg
      361 acaagtcata tccgtacctg aaaatcacca atcaagacgt gccgcgcatg gcctactacc
      421 ggggcggcgt ggacaagcgc caccagtatt tcgggccgtt tccgagcgct tgggcggtga
      481 aggagtcgat ccagatcctg cagaaggtgt tccaactgcg cacctgcgag gacacggtgt
      541 tcgcgaaccg cacgcgtccc tgcctgctgc accagatcca gcgctgctcg gcgccgtgcg
      601 tgaacgcaat cagccgcgaa gactacgcgc tcgacgtgga gaacgcggcg aagttcctgc
      661 gcggccgaca gggcgaagtg atggagagcc tggagcagaa gatgcacgcc tatgcggcgg
      721 aactgaagtt cgagcaggcg gcggcggtgc gcaaccagat cagcgcgctg tcgagagtgt
      781 tgcaccagca gagcatggag acggtcggcg acgcggacat cgacatcatc gccgcgatcg
      841 tgcagggcgg gcgggcctgc gtcaatctgg cgatggtgcg cgggggcagg catcttggcg
      901 accgcgcata ctttcctacg catgtcgagg atgccgtcgc tgcggccgag gacagtgtcg
      961 agctggaagt gctcaaggcg tttctggcgc agcactacat cgacaagttc atcccgtcga
     1021 cgctgatcct gaatctcgag atggacgagc cagaattgat gacggccctg atggagcagt
     1081 gcggccatcg catcaatctc gtgttccagc cgcagggcca gcgcaggcag tggctggaga
     1141 tggcgcagaa gggcgccgag atcgcgctcg cgcggctgct gtcggagcag ggatcgcaac
     1201 aggcgcgtac gcgcgcgctg gcggaggtgc tcggtttgga gacggacgat ctggacgcgt
     1261 tgcgcatcga gtgcttcgac gtcagccaca cgcagggcga ggcgacgcag gcttcctgcg
     1321 tcgtctttca tcatcacgcg atgcagaacg gcgagtaccg ccgcttcaac atcaccggtg
     1381 tcgctccggg cgacgactac gcggcgatgc gccaggtgct gagccgccga tacgagaagg
     1441 tggcaggcgg cgaaggcgtg ctgccggaca tcgtgctggt cgacggcggc aaggggcagg
     1501 tcgagatcgc gcgccaggtg ttttccgagt tcggtctcga taccggcatc atcgtcggtg
     1561 tcgccaaagg ggaggggcgg cgtgtcggac tggagacgct gatcttcgct gacggccgtg
     1621 ttccgcttga gctgggcaag gagtctgcgg cgctgatgct gatcgcgcag atacgcgacg
     1681 aggcgcatcg cttcgcgatc acagggatgc gggccaagcg cgcgaaggcg cgtcaggcct
     1741 cgcggctgga agagatcgaa gggattggat caaagcggcg ccagcgtctg ctggcgcgct
     1801 tcgggggagt gcgcggcgtg atggatgcca gcatcgagga actcgcttcc gttgaaggca
     1861 tttcgcgcca gctcgccgag cagatctacc ggcagctgca ctaggccgga caagctgtgg
     1921 tgcggcaaac atcggtgaat ccgccggatg tttgcagagc tgccaagttc cacggataat
     1981 ccgcacaacc ttttggcggc cattatgccc ttcaacattc cgatcctcct gacctggctg
     2041 cgcgtggcat tgataccgct cgtggccggc gtgttctatc tgccagagac tttcctttcg
     2101 cgcgaaatgc agggtctcgc atccgccttg atttttatcg tcgccgccgt gaccgactgg
     2161 ctcgacggtt atctcgcgcg acgctggaac gagacgtcgg cattcggcgc atttctcgac
     2221 ccggtcgccg acaagctgat ggttgccggc gccttgctgg ttctcgtcga gctccaggca
     2281 gtcaacgcgg ttgtcgcatt catcatcatc ggccgcgaga tcgcgatctc ggcgctgcgg
     2341 gaatggatgg cgcagatcgg cgcgtcgaag tcggtcgccg tcagttcgct cggcaagatc
     2401 aagaccgccg cgcagatgat tgcgatcccg atgctgctct acagcggcat cgttgccggc
     2461 atcgacctgc gtcatctcgg tgcctggctg ctggtcatcg ctgccgtgct gacggtatgg
     2521 tcgatgttct attacctgcg caaggcctgg ccgctgatca aggagaaggc ggcacgcgcg
     2581 cattgacggc agttgtgcct gacatgacgc ttgacaattt catgtggggc tttataatgc
     2641 ctgactgttg tgaacgcggg agtagctcag ttggtagagc gcaaccttgc caaggttgag
     2701 gtcgagagtt cgagactctt ctcccgctcc aggtttcttg aaaaaggaag cgcagcgagc
     2761 ttcctttttt gtttggtgtt agctcctctc cggacagctt tgcatcagca ggtcaatgcg
     2821 ggaatagctc agttggtaga gcgcaacctt gccaaggttg aggtcgagag ttcgagactc
     2881 ttttcccgct ccagtcttcc ggtatcgctg cagatcgcgg ttccgggttt gtggttaaaa
     2941 taccttccct cggcggggta gcaaagtggt tatgcagcgg cctgcaaagc cgtctacgcc
     3001 ggttcaattc cgacccccgc ctccagtttc aaaaaagcgc cgcgtgcgct ttttttttgt
     3061 tcgctgatca cgcgagcccg agtggtgaaa ttggtagaca cagcggactt aaaatccgcc
     3121 gcgcctaatc agcgcttgcc ggttcgatcc cggcctcggg caccaatccc tggcctctct
     3181 cgtggtgcct gccccgccac cggtcctggg taatgccaca actctccgcg ccgaccctcg
     3241 cacctgcaca aattctcatg cgtatggcgg caccatcgaa gcaccgtggc gtcttatgga
     3301 tcagcaaagc cgcatcgatc ggagcatcca tggcgaattt ccccctgagt cgtcgtcgcc
     3361 gctggtgggg cggtgcgatg ttcgccgcca tgctcgtcgc acctggcatt accgttgcgg
     3421 acgacgtcgg ctcggcgtca tcgtctcagc aacagcagat cgacgatccg cgcactcagc
     3481 tgcagttgct gcagagccag actcaatacc aggaaatgca ggcgcgccac gaagaggtgc
     3541 ggcagcggat ggaaatggag gcgcgcatgc ggcagcagga aacgcagcag cacgtccagc
     3601 agcggattcg ggaacagaga atggagatgc agatgcatgc tcacgagcag aggagacagc
     3661 tcgagcagga gcggcgtcgc tagggtattc ccgcggcttt ggacaacatg tcgcttgcag
     3721 ccgcgcgtcc cttgcttacg cttgcgccgt cttcgatcga gcgtcttgct gccgcgccac
     3781 gttcagatcc tccgcaatac gacgatgtgc gtgcccttcg ccaggtcggc gttcgtgccg
     3841 aaaaaatagg gaacatcctc gcttgctccg ctgtcattgg tctgtatgta tatacagtga
     3901 ggcgagagat ggacaagcat acgcaggagc aggctgaaca cagcaacatg gtgaaggacc
     3961 cgaaggactg gacgaccggg gacgagccga tgacgggtgc acagaagtcg tatctgaaga
     4021 cgctgtcgga ggaagcgggc gtgccgatgg acgaaaacct gagcaaggcc gaggcatcgc
     4081 agcggatcga tgaactccag caacagaccg ggcgcggcat gacggggatg caccggcatt
     4141 gagtgtcatc gccggttcca gctcgactag aaccggtaga acaggccggc gcgtatgacg
     4201 gggaagaagc gcaggtccgg cagttctccg cgcaggcgcg gcacttcatc gagcaggtcg
     4261 acgttcaact gctcgcacag cccggggact tcgacgcgac aatccggtgc ccatagcctt
     4321 gcgcttcgcg catgcccctg atagaacacg ccgatgtcga cggcgacgct ccagccgcca
     4381 tactgcgacg gcggggtcgt caggccgacc ccgatgaagg gagccaggcg gttgaagtcg
     4441 acgcgccctc gtacctcgcc aacctcgagt acatcgtact cgcgcccgcc gatgcggtag
     4501 ctgagatcgg gatccggccg cgccaccgcg gcgacgtgat tgccattgat gacgagaccg
     4561 gtcgagacgc ggaacatgtt gccccacgga tgccaatcga ggaggagttc ggcggtgcgg
     4621 atgttcctgc gggatgtata ggccagcgtg ccggcgctgc gctcctcgcg caggtcggga
     4681 agggcgttga ccgaggtccg cacgctcagc ttgtgatgga cagggaccga aacatgcagc
     4741 ccgcccccgg tcgttcccag cagcacgccg gcatcggccg cgcc
//
LOCUS       DATLGF010000344         5381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_46656,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000344 DATLGF010000000
VERSION     DATLGF010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46656"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /gene="cpaB"
                     /locus_tag="VM406_10535"
     CDS             complement(<1..513)
                     /gene="cpaB"
                     /locus_tag="VM406_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HVL76442.1"
                     /translation="MKNLKAFGLLLLAALFGLAAAVYTAGWVSKQAGIASNRIVVAAV
                     DIQLGSKISPQMLVTADWPSGSIPAGAFTDLNALQERVLKVSVLRGEPILEGKLAPVG
                     TRGGLSAVIAEGKRAMTVRVNDVVGVAGFALPGNYVDVMVNTERDVGDGSRTSRQISK
                     TVLENVLVLAV"
     gene            complement(536..1150)
                     /locus_tag="VM406_10540"
     CDS             complement(536..1150)
                     /locus_tag="VM406_10540"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HVL76443.1"
                     /translation="MQLLSMDQSAGSELAAAFELLAMAAGEPRLILLVTLLAFAAWSD
                     VRSYRIPNWLSLGGTATALLFAFLLPATFGITLQQGLFGMVAALLVMLPLYALRAMGA
                     GDAKLMAMVGTFLGPLETLYALLLTFALAGLYALLYAAARRSLSRMFVNVRDLAQTML
                     LDAAFGKLRPAPVALGQSVGRLPYAVCIGVATIASLIARQLGFL"
     gene            complement(1592..1984)
                     /locus_tag="VM406_10545"
     CDS             complement(1592..1984)
                     /locus_tag="VM406_10545"
                     /inference="COORDINATES: protein motif:HMM:NF020903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HVL76444.1"
                     /translation="MKIMKCARTAHTPEVVLDTEAGTLLIEGECYPENPATFFQPVIG
                     AVQMVLASGSLKQFHARFRLAYVNSASTKSMRQLFLILNEAAKAGCNVDVAWEFDEDD
                     DAIQDLGEDLMYGLGIPYLAFHETPYVA"
     gene            complement(2004..2555)
                     /locus_tag="VM406_10550"
     CDS             complement(2004..2555)
                     /locus_tag="VM406_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HVL76445.1"
                     /translation="MDFKAFGAFHGGAADAGVIFFYTGAFDSEVVNALSSQLKERLEA
                     ESASAAVKRKLFSTFIEMAQNVLHYGGAPAPGNKPGSIGMGREGESYWVACGNLVAHE
                     HVQRLNDKLETLRNMSLAEIKAAYREQLANDAHEANDAISKGAGLGLLTIARDSGHPI
                     AFRFEADPASGGHFSYFHLKTVI"
     gene            complement(2558..3838)
                     /locus_tag="VM406_10555"
     CDS             complement(2558..3838)
                     /locus_tag="VM406_10555"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HVL76446.1"
                     /translation="MASIEDLYSSIANDSAAQSGTQAGLALRAGDLASAAPTVPPDCS
                     NAQLQEFFHAHPERDTVAVVADGAPLGLVNRNHFMEQYGKPFSRELFGKASCLSFADA
                     APLITPFETPIEVLVRHAVAPNSRVMKDGFICTRDGQYCGTGSGMTLLKAMSDIEAEK
                     TRQLLSSIDYASAIQQSNMRASMSELQQGLPDSRLVWLPRDVVGGDCYFFRATRDGMF
                     GAIIDCTGHGVPGAFMTLIALSFLESRIAGGDEAPGPARILGELNRHIKKVLGQDATA
                     KDGIQAAMKSDDGLDAFMFVLSRDRRTLSYAGARLEMAIAVPGEDEVRLLEGDKMGVG
                     YSDTPDDFAYTTSTVVLPPDSRVLVTTDGIIDQIGGPKNIAHGRKRLYRLLAQRRTLA
                     PADLAAVLLEQFAEWQGEQRRRDDVCFLTFAAGA"
     gene            complement(3913..4509)
                     /locus_tag="VM406_10560"
     CDS             complement(3913..4509)
                     /locus_tag="VM406_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76447.1"
                     /translation="MLPAKASATMPACSGCHCPPLSRSSMQPIRELEANIVFSKTARG
                     QEEVLTRSHGLAARHRQVLILINGARSLGTIAEIIPASELAEIVGFLLEARFIEPAQG
                     AESSSRAAVITATSVSASGDTVAKVKEFMVASAQSCLGLLAVDVIRQIEIAQDKPALM
                     TAVAQWHMALRESREGKRLAGPYLDQVRIALAAGGMES"
     gene            complement(4690..4765)
                     /locus_tag="VM406_10565"
     tRNA            complement(4690..4765)
                     /locus_tag="VM406_10565"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4730..4732),aa:Lys,seq:ttt)
     gene            5080..>5381
                     /locus_tag="VM406_10570"
     CDS             5080..>5381
                     /locus_tag="VM406_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HVL76448.1"
                     /translation="MILNRLNSADTTLPLTQFSASGESVEERSSRCAACSMHKICLPT
                     GLSEEDMARLDKIIGRRRKVPRDGHLYRVGDPFTNLYAIRLGHFKTHQINPNGDQQ"
BASE COUNT         1049 a         1701 c         1696 g          935 t
ORIGIN      
        1 caccgcgagc acgagcacgt tctccaggac agtcttgctg atctgccggc tggtcctgct
       61 gccgtcgccg acatcgcgct cggtattgac catcacgtcc acataattgc caggcaacgc
      121 gaaacccgcc accccgacca cgtcgttgac ccgcacggtc atcgcgcgct tgccttcggc
      181 aatgacggcg gaaaggccgc cgcgcgtgcc gaccggcgcg agcttgccct ccaggatcgg
      241 ttccccgcgc agcacactga ccttgagcac gcgctcctgc agtgcattca agtcggtaaa
      301 agcgcctgcc ggaatcgagc cgctaggcca gtccgcagtg accagcatct ggggactgat
      361 cttgctgccg agctggatgt cgactgccgc cacgacgatg cgattggaag cgatcccggc
      421 ctgcttcgat acccaccccg cggtgtatac cgcggccgcc agtccgaaca gtgccgcgag
      481 caggagcaag ccgaaggcct tcaggttctt catgtctacc cttgagtgat cgttgttaca
      541 ggaagccgag ctggcgcgcg atcaggctgg caatggtcgc gacccctatg cagacggcat
      601 aaggcagcct cccgacagat tggcctagcg cgaccggcgc cggtcggagc ttgccgaagg
      661 cggcatcgag gagcatggtt tgtgcgaggt cgcgcacatt gacgaacatt cgggagagcg
      721 agcggcgcgc agcggcatac agtagagcgt agagaccggc gagcgcaaag gtaagcaaca
      781 acgcgtatag cgtctcgagg gggccaagga aagtgccgac catggccatc agcttcgcgt
      841 caccggcacc cattgcccga agggcataaa gcggcagcat gaccagaagc gcagcgacca
      901 tgccaaagag cccttgttgg agtgtgatcc cgaacgtggc gggcaggaga aaggcgaaca
      961 gcaaggccgt tgcggtcccg cccagactga gccagttcgg gatccggtag ctgcggacat
     1021 cggaccacgc agcgaacgcg agtagcgtca cgagcaagat caggcgcggt tcgccggccg
     1081 ccatcgccag cagttcgaaa gccgcagcga gttccgagcc tgcggactga tccatggaaa
     1141 gaagttgcaa cgtacgcctc cctgaacatg gcgttgcttc ttgttatcag tcggcgcgat
     1201 ccccttgatc cgcatcgacc aggcgcttga aaaaagataa gcgcttgaga tgcattgaat
     1261 gacttttggt tactttcaat tttgacatcg aacaagaaac gagcattggc tgaatgagcc
     1321 aaacccgcgt cccgcaagcg tttcaagcat gaagcacatg cacccccgtg gtgcagaccc
     1381 ggcacctcgt tgcctgagaa acaccttcct tttggtaatt gttagtttct gatgggcaca
     1441 ggccgctgcc gccgccacca ccgccgaagg cggcgcggcg caggatcggg aaggcgaagg
     1501 aacgaagagg aagggttagg ctcagggctc actgagcaaa accggcgccg cgccgatggg
     1561 cgagcgacgg ttgttcagcc gccgctcgcg gttatgctac gtacggagtc tcatggaagg
     1621 caaggtaggg aatgccgagg ccgtacatca agtcctctcc cagatcctgg atcgcgtcat
     1681 cgtcctcatc gaactcccag gccacgtcga cattgcaacc tgccttggcc gcctcattca
     1741 ggatcaggaa cagctgccgc atcgacttcg tcgacgcgct gttcacatag gcgagccgga
     1801 agcgggcgtg aaactgcttc aggcttcccg atgcgaggac catctgcacc gcgccgatca
     1861 ccggctggaa gaaggtcgcg ggattctccg gatagcactc gccctcgatc agaagcgttc
     1921 ccgcttcggt atccagaacc acttccgggg tatgcgcggt gcgcgcgcat ttcatgatct
     1981 tcatggtgtt cggctcctgt gcatcagatg acagtcttga ggtggaaata ggagaagtgc
     2041 ccgcccgagg cgggatcggc ttcgaaacga aacgcaatcg gatggccgct gtcgcgcgcg
     2101 atcgtcagca ggccgaggcc cgcgcccttg ctgatcgcgt cgttggcctc gtgcgcgtcg
     2161 ttcgccagct gctcacggta ggcggccttg atctcggcca gcgacatgtt gcgcagcgtt
     2221 tcgagcttgt cgttcaggcg ctgcacgtgt tcatgggcga ccaggttgcc gcaggcgacc
     2281 cagtaggact cgccttccct tcccattccg atcgaacccg gcttgttccc gggtgcgggc
     2341 gcgccgccgt aatgcagcac gttctgcgcc atttcgatga aggtggagaa cagcttgcgc
     2401 ttgaccgcgg cgcttgcgct ttcggcctcc aggcgctcct tcaactggct cgacagcgca
     2461 ttcaccacct cgctgtcgaa cgcgccggta tagaagaaga tcacgccggc gtcggcggcg
     2521 ccaccatgga aggcgccgaa tgccttgaaa tccatgatca ggctcccgcg gcgaacgtga
     2581 ggaagcacac gtcgtcgcgt cgccgctgct cgccctgcca ttccgcgaac tgctcaagca
     2641 gcacggcggc aaggtcggcc ggcgccagcg tgcgccgctg cgcgagcagg cgatacaggc
     2701 gcttgcgtcc gtgggcgatg ttcttgggtc cgccgatctg atcgatgatg ccgtcggtgg
     2761 tgacgagcac acgcgagtcc ggaggcagca ccaccgttga cgtggtgtat gcgaagtcgt
     2821 ccggcgtgtc gctgtatccg acgcccatct tgtcgccttc gagcagccgc acctcgtcct
     2881 cgccgggtac ggcgatagcc atctcgaggc gcgcacctgc atagctcagc gtgcgccgat
     2941 cgcgcgacag cacgaacatg aaggcgtcga ggccgtcatc cgatttcatc gcggcctgga
     3001 tgccgtcctt cgccgtcgcg tcctgaccga gcactttctt gatatgccgg ttgagctcgc
     3061 cgagaatgcg tgcgggaccg ggcgcctcgt cgccgccggc aatgcggctc tcgaggaagg
     3121 acaaggcgat cagcgtcatg aacgcacccg gcacgccgtg accggtgcag tcgatgattg
     3181 cgccgaacat tccatcgcgc gtggcgcgga agaagtagca gtcgccgccg accacgtcgc
     3241 gcggcagcca tacgaggcgc gaatccggca gcccttgctg caattcgctc atggaggcgc
     3301 gcatgttcga ctgctggatc gccgaagcgt agtcgatgct cgagagcagc tggcgcgttt
     3361 tctcggcctc gatgtcggac atcgccttca gcagcgtcat gccggaaccg gtgccacagt
     3421 actggccgtc acgcgtgcag atgaagccgt ccttcatcac gcgcgaattc ggcgccaccg
     3481 cgtgccgcac cagcacttcg atcggcgtct cgaacggcgt gatgagcggc gccgcatcgg
     3541 cgaaagacag acagctggcc ttgccgaaca gctcgcggct gaaaggcttg ccgtactgct
     3601 ccatgaagtg gttgcgattg accaggccaa gcggtgcgcc atcggcaacc acggccacgg
     3661 tatcgcgctc gggatgagcg tgaaaaaact cctgcagctg cgcattgctg cagtccgggg
     3721 gcacggttgg cgcggccgac gcaagatcgc ccgcgcgcag cgccagcccg gcttgcgtac
     3781 cgctttgcgc cgcgctgtca ttcgcgatgg agctgtacag gtcttcgata ctcgccacgc
     3841 agtgtccttt cgtattcggt tgcgatggcg gcactataaa gtttctagat gacaacagta
     3901 tgaaacgcgg cgtcacgact ccatgccgcc tgcggccagt gcgatcctga cctgatccag
     3961 atacgggcct gcgagccgct tgccttcgcg cgactcgcgc agggccatgt gccactgggc
     4021 cacggccgtc atcaacgcag gcttgtcctg cgcgatttcg atctggcgga tgacatcgac
     4081 agcaagcagg ccgaggcagg actgggcgct ggccaccatg aactccttca ccttggcaac
     4141 cgtgtcgccg gacgcagata cggacgtcgc agtgatgacg gcggccctgg aggaggactc
     4201 ggcgccttgc gccggctcga tgaaccgcgc ttcgagcagg aagcccacga tttcggccag
     4261 ctcgctcgcg ggaataattt ccgcgatcgt gcccagactg cgggcgccgt tgatcaggat
     4321 aagcacctgc cgatggcgcg ccgcgaggcc gtgactgcgt gtcaaaacct cctcttggcc
     4381 gcgcgcggtc ttggagaaga cgatgtttgc ttcgagttcg cggattggct gcatggacga
     4441 gcgggaaagt ggcggacagt gacaaccgga acaggctggc atggtcgcgg atgcctttgc
     4501 tggcaacaca ctaacgcatg attgttacgc ccatgtttca ggaaacaacg ggagcatcgc
     4561 agcgggggca aagacgggag agcgcggggg gtgctgaagc agggaaaacg caaggaggaa
     4621 gaacgtgcga tcgccggggc cggaaaacag aaggggccca ggctatgcgc ctgggcccct
     4681 cggtatttct ggtaggccgt gcgggactcg aacctgcgac caacggatta aaagtccgct
     4741 gctctaccaa ctgagctaac gacccgaaag accgcgatta tagtaaggat tgggcgaggg
     4801 tgtcaaaccg gcatccgtgc aagttgacgg cgggcgccgt gccgattccc ggaaatgaga
     4861 gagcgggcgg gctcacgctg cctgaaacct ccggcagggc gcgcgctcct ccagctcgcg
     4921 cccgccctgc cgacatcgca ttttccctct gaggcgggag aaaattcgcc ctgaatctgc
     4981 gcccgtcgcc gcagggagga acgggctctg gcacaccgct tgctgtagtg caggtgtcgc
     5041 ccaccgttgt ggccgaaata accagaaacg agaacagcca tgatcctcaa tcgcctgaac
     5101 agcgccgaca cgaccctgcc cctcacccag ttttccgctt ccggcgaatc cgtggaggaa
     5161 cgcagctcgc ggtgcgccgc ctgcagcatg cacaagatct gcctgccgac cggcctgtcc
     5221 gaggaagaca tggccaggct cgacaagatc atcgggcgcc gccgcaaggt gccgcgcgac
     5281 ggccacctgt accgcgtcgg cgacccgttc acgaacctgt atgcgatccg cctcggccat
     5341 ttcaagaccc accagatcaa tccgaacggc gaccagcaga t
//
LOCUS       DATLGF010000345         8157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_229901, whole genome shotgun sequence.
ACCESSION   DATLGF010000345 DATLGF010000000
VERSION     DATLGF010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229901"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1732
                     /gene="ftsH"
                     /locus_tag="VM406_10575"
     CDS             <1..1732
                     /gene="ftsH"
                     /locus_tag="VM406_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HVL76449.1"
                     /translation="VKARNIQEVMIEGTSITATTSNGTRLATNTTMLDRGLISDLIAN
                     DIKFDVRHPEQPNFLLQVLISWFPILLLIGVWIWFMRRQQGGGKGGVFSMGKSKARMQ
                     DEHTNTTTFADVAGCDEAKEEVDEIVEFLKDPRKFQSLGGHIPRGVLLVGPPGTGKTL
                     LARAMAGEARVPFFTISGSDFVEMFVGVGASRVRDLFEQAKKHAPCIIFIDEIDAVGR
                     SRGSGMGGGNDEREQTLNQMLVEMDGFEPNSGIIVIAATNRADVLDKALLRPGRFDRQ
                     VMVGLPDIRGREQILNVHMRRVPVDADVRADVLARGTPGFSGAELSNLVNEAALFAAR
                     RNKQTVDMQDFEDAKDKIIMGPERKSAVMREEERRNTAYHESGHAVVAKLLPKADPVH
                     KVTIMPRGYALGLTWQLPEHDRVNMYKDKMLEDIAILFGGRIAEEIFMNQKSTGASND
                     FERATKLARAMVTRFGMSEVLGTMVYTDEQDGFGLSSRTVSEETQQKVDEEIRSILDT
                     QYALARRLLEENREKVDVMAKALLEWETLDADQVNDIMEGKEPRAPKYLPTKKTPPRT
                     GGMGPLVQPA"
     gene            1886..3076
                     /locus_tag="VM406_10580"
     CDS             1886..3076
                     /locus_tag="VM406_10580"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVL76450.1"
                     /translation="MDSSFDAARQHFFTGIEHFEAGRLEPACAAFATAAALAPGRPSV
                     LGNLGITLFQLGRIDEAVAPLRQATAADPAYAEAWACLGLAEEARGRWQEAADALEQA
                     LRLAPDRAPLWMSLALCRQRLGQAGPALDAFGRALAADPAYADAWSARGSLLRELGRL
                     AEAAQCFEQALACGGDPELNRYYLASVQEAGLPPAPPRRYVESLFDEYAPEFQGHVTG
                     ALRYQGHEVLLRPLLQAGRRYPRVLDLGCGTGLCGTLIAPAADAIVGVDLSQAMLEEA
                     RKSGVYRELVHADVASFLAGERSPVDLVVAADVFIYVGALDAVFGAVRRILAPGGCFA
                     FTVEEPVEDGGMQLLPSLRYAHSEQYVRDLAQRNGFEVDAMSRAPIRHDQERPVGGLY
                     VYLR"
     gene            complement(3141..5795)
                     /locus_tag="VM406_10585"
     CDS             complement(3141..5795)
                     /locus_tag="VM406_10585"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL76451.1"
                     /translation="MTQPLHPTSLPFIARPGAMAELMRSQDWSATPLGAPQTWPATLR
                     TACGIVLNSDFPMFLLWGRDLVCLYNDGCIPLLGNKHPGTLGRPFPVLWPEAWPALGS
                     AIEHAMNGEPVYCRDVPVDLTRDGRTERSWFTFSCSPLFDDSGQVCGLLGICIETTER
                     MREDMRQALVLETVDSLRNLTKVDEITETAAALLGRRLEAPLVFYSEIDDAHGTFEIK
                     RGWASERVCPVVGQCGKLSDFGPQLIEDLHAGRPICIKDVRTDSRTRDFAAAYAALGL
                     RSGLLVPLLKDRALVGVLNVYDVVPRDWSDADHKVVAAVGERIRLAMEQARSEAALRA
                     AANRQAVLLELGDALRNLADDPQAMMDASAHALARALDVPRVGYADVDEGVEYAVVGE
                     NYNDTSRIPDVRAHRDRLDNYGSALVEDIRAGRVMRVDDIAADPRSAGAAASAHEAIG
                     ARASMAVPVQRNGRTVAFMFAHHDKPRHWTDEEAALMREVADRSWAAVERARAELELR
                     EASRRKDEFLAMLAHELRNPLAPISSAAELLRMARLDPERIEKTTDIIVRQVKHMTGL
                     IDDLLDVSRVTRGLISLDCREVEIRHVIAEAVEQVRPLITSRRHHLSTHMAPGPGLVN
                     GDRKRLVQVFSNLLANAAKYTPEGGEITVKMDVSEREIAVRVCDTGIGMASELLPKVF
                     ELFAQAERTPDRSQGGLGLGLALVRSLAQLHGGRVQAASPGPGKGSVFTVTLPRVLAA
                     QSGAGDAQTGTAQRQTASLDLLVVDDNQDAAQTLALLLENAGHRVCVAYSPQAALEAA
                     QRRTFDGCMLDIGLPGMDGLELVRRLRMLPGTRAALMVAVTGYGAQGGQQRAQDAGFN
                     HYLVKPVNPARLLGLLADAGEAPAHAAH"
     gene            6154..6738
                     /locus_tag="VM406_10590"
     CDS             6154..6738
                     /locus_tag="VM406_10590"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HVL76452.1"
                     /translation="MSLAQFIHEYGYFAVAIGSFLEGEAVLLAGSFAAYAGHLDLPLV
                     LAIAALASFLGDLPYFFAGRRYGSAVLQRFPALHARKGRLDKLLQRHHVLLVLSLRFL
                     YGLRIAGLLTLGMSRMSAWRFLALDFLGAVIWSSTVCATGYGVGRLAQAMFGDISTGE
                     QISLLLAVLVCASLLIMLARRRLALAGVDRPPGH"
     gene            6766..7032
                     /locus_tag="VM406_10595"
     CDS             6766..7032
                     /locus_tag="VM406_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HVL76453.1"
                     /translation="MQREEFLARLAEDGFKEVVTVEREANGAWELHAHPFEAKALILR
                     GEIRLRRGEEERTYPAGTIFHLAADEPHSEWYGPQGVTYLVGRR"
     gene            complement(7033..>8157)
                     /locus_tag="VM406_10600"
     CDS             complement(7033..>8157)
                     /locus_tag="VM406_10600"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012338582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HVL76454.1"
                     /translation="LQNIAQLPIVHPHVAAMPDVHAGIGATVGSVIPTRSTVIPAAVG
                     VDIGCGMNAVRTTLTASDLPDSLARLRGAIEGAVPVGFAQHEWNKVRGAAHARAARPL
                     SDRLDRIIGRHRGIAKMQQKFEQTWICQLGTLGGGNHFIELCLDEEQRVWIMLHSGSR
                     GIGNAIGRYFIAAARKEMERHQIRLPDRDLSYFREGSELFDDYVEAVEWAQDYALVNR
                     REMMRRVVDVLIQQLPPFRLDGEAINCHHNYVSRETHGGERLFVTRKGAISAREGELG
                     IIPGSMGARSYVVRGKGNPDSFCSCSHGAGRRMSRTEAKRRFNRFDLAEQTRGIECRK
                     DGGVIDEIPAAYKDIDAVMAHQTDLVEVVHTLRQVVCIKG"
BASE COUNT         1273 a         2753 c         2765 g         1366 t
ORIGIN      
        1 tgtgaaggcg cgcaacatcc aggaggtgat gatcgaaggc acgagcatca ccgccaccac
       61 cagtaacggt acgcgcctgg ccacgaacac cacgatgctc gaccgcggcc tgatcagcga
      121 cctgatcgcc aacgacatca agttcgatgt gcgccatccc gaacagccca acttcctgct
      181 gcaggtgctc atttcctggt tcccgatcct gcttttgatc ggcgtgtgga tctggttcat
      241 gcggcgccag cagggcggcg gcaagggcgg cgtgttctcg atgggcaagt ccaaggcgcg
      301 catgcaggac gagcacacca acaccaccac cttcgccgac gtcgccggct gcgacgaagc
      361 gaaggaggaa gtcgacgaga tcgtcgagtt cctgaaggat ccgcgcaagt tccagagcct
      421 gggcggccac atcccgcgcg gcgtgctgct ggtcggcccg ccgggcaccg gcaagacgct
      481 gctggcccgg gcgatggccg gcgaggcgcg cgtgccgttc ttcacgattt ccggttccga
      541 cttcgtcgaa atgttcgtcg gcgtgggcgc ctcgcgcgtg cgcgacctgt tcgagcaggc
      601 gaagaagcat gcgccctgca tcatcttcat cgatgaaatc gatgcggtcg ggcgttcacg
      661 cggcagcggc atgggcggcg gcaacgacga gcgtgagcaa acgctcaacc agatgctggt
      721 cgagatggac ggcttcgagc cgaactccgg catcatcgtg atcgccgcga ccaaccgcgc
      781 cgacgtgctc gacaaggcgc tgctgcgccc gggccgcttc gaccgccagg tgatggtcgg
      841 cctgcccgac atccgcgggc gcgagcagat cctgaacgtg cacatgcgcc gcgtgccggt
      901 cgacgcggat gtacgcgccg acgtgctggc gcgcggcacc ccgggcttct ccggcgccga
      961 gctgtccaac ctggtcaacg aagccgcgct gttcgccgcc cgccgcaaca agcagacggt
     1021 ggacatgcag gatttcgagg atgcgaagga caagatcatc atgggtccgg agcgcaagtc
     1081 cgcggtgatg cgcgaggaag agcgccgcaa cacggcctac cacgagtccg gccatgcggt
     1141 ggtcgccaag ctgctgccga aggccgatcc ggtgcataag gtcacgatca tgccgcgcgg
     1201 ctatgcgctg ggcctgacct ggcagctgcc cgagcatgac cgcgtgaaca tgtacaagga
     1261 caagatgctg gaagacatcg cgatcctgtt cggcggacgc attgcagaag agatcttcat
     1321 gaatcagaag tcgaccggcg cctcgaacga cttcgagcgg gcgaccaagc tcgcgcgtgc
     1381 gatggtgacc cgcttcggca tgtccgaggt gctcggcacc atggtctaca ccgacgagca
     1441 ggatggtttc ggcctgtcct cgcgcacggt gtcggaagag acccagcaga aggtcgacga
     1501 agaaatccgc agcatcctgg acacgcagta tgcgctggcg cgccggctgc tcgaggagaa
     1561 ccgcgagaag gtcgacgtga tggccaaggc gctgctcgaa tgggagacgc tggacgccga
     1621 ccaggtcaac gacatcatgg aaggcaagga gccgcgtgcg ccgaagtatc tgccgaccaa
     1681 gaagactccg ccgcgcaccg gcggcatggg accgctggtc cagccggcct gacggccttc
     1741 aaaggtcccc gctaacggac cgccgacctt cgggtcgcgg tccgttggtc ttttgggggc
     1801 gggctttgtc gcagggcgtc cccgcaacgc tctccaatgc gggagaatgg cgtactttcg
     1861 atcaacgcca cgtcatccgc gcaccatgga ttcttccttc gacgcagcac gccagcattt
     1921 cttcaccggc atcgagcatt tcgaggccgg gcggctggaa ccggcgtgcg ccgccttcgc
     1981 gactgccgcc gcgctcgctc cgggccgccc ttcggtgctc ggcaatctcg gcatcacgct
     2041 gttccagctc ggccgcatcg acgaggccgt ggcgccgttg cgccaggcca cggccgccga
     2101 tcctgcatac gcggaggcct gggcctgcct gggcctggcc gaggaggcgc gtgggcgctg
     2161 gcaggaggcc gccgacgcgc tggaacaggc gctgcggctg gcaccggaca gggcgccgtt
     2221 gtggatgagt cttgcgctgt gccggcagcg cctcggacag gcgggaccgg cgctggacgc
     2281 cttcgggcgc gcgctggccg ccgaccccgc atacgccgat gcctggagcg cgcgcggcag
     2341 cctgctgcgc gagctcgggc ggctagccga ggcggcgcag tgtttcgagc aggcgctcgc
     2401 ctgcggcggc gaccccgaac tgaaccgcta ctacctggcc tcggtgcagg aagcgggctt
     2461 gccgcccgcg ccgccgcgcc gctacgtgga atcgctgttc gacgagtacg cccccgagtt
     2521 ccagggccac gtgaccggcg cgctgcgtta ccagggccat gaggtgctgc tgcgaccgct
     2581 gctgcaggcg ggccggcgct acccccgcgt gctcgatctc ggctgcggca ccggcctatg
     2641 cggcacgctg atcgcgccgg cggccgacgc gatcgtcggc gtcgacctct cgcaggcaat
     2701 gctggaagag gcgcgcaagt ccggcgtgta ccgcgaactc gtgcacgccg acgtggcgtc
     2761 cttcctcgca ggcgagcgca gtccggtcga cctggtggtg gcggcggacg tgttcatcta
     2821 tgtcggcgcg ctcgacgcgg tgttcggcgc ggtgcggcgc atcctggcgc cgggaggctg
     2881 ctttgcgttt accgtggaag agccggtcga ggacggcggc atgcaactgc tgccgagcct
     2941 gcgctacgcg cattccgaac agtacgtgcg cgatctcgcg caacggaacg gcttcgaggt
     3001 ggatgcgatg tcgcgcgcgc cgatccgcca tgaccaggag cgcccggtgg gcgggttgta
     3061 tgtctacctg aggtaggccc ggtcgggagc gagccactgt gcgcgccggc gcgcgttgcg
     3121 atctgccctt gcagtgtctc tcagtgcgcc gcgtgcgccg gcgcttcacc ggcgtcggcg
     3181 agcagcccga gcagccgtgc cggattgacc ggcttgacga ggtaatggtt gaagcccgcg
     3241 tcctgcgcgc gctgctgccc gccctgggcg ccatagccgg tgacggccac catcaatgcg
     3301 gcacgggtac ccggcagcat gcgcaggcgg cgcacgagct cgaggccgtc catccccggc
     3361 aacccgatat ccagcatgca gccgtcgaat gtccgccgct gcgccgcttc gagcgcggct
     3421 tgcgggctgt aggcgacgca cacccggtgg ccggcattct ccagcagcag ggcgagcgtc
     3481 tgcgccgcgt cctggttgtc gtcgacgacc agcaggtcga gggatgccgt ctgccgctgc
     3541 gcagtgccgg tctgcgcgtc gccggcgccg gactgcgccg ccagcacgcg tggcagggtc
     3601 acggtaaaca cgctgccctt gccgggcccc gggctggcgg cctggacgcg tccgccatgc
     3661 agctgggcca ggctgcgcac cagcgcgagt cccagtccca ggccaccctg cgagcggtcc
     3721 ggcgtgcgct cggcctgggc gaacagctcg aacaccttgg gcagcagctc gctcgccatg
     3781 ccgataccgg tatcgcatac ccgcaccgcg atctctcgct cgctgacatc catctttacc
     3841 gtgatctcgc caccttccgg cgtgtacttg gctgcgttgg cgagcaggtt ggagaacacc
     3901 tgcaccagcc gcttgcggtc gccgttcacc agcccgggac cgggcgccat atgcgtgctc
     3961 aggtgatgcc ggcgcgaagt gatcagcggg cgcacctgtt ccaccgcttc ggcgatcaca
     4021 tggcggattt cgacttccct gcagtccagc gagatcagcc cgcgcgtcac gcgcgagacg
     4081 tcgagcaagt cgtcgatcag gccggtcatg tgcttgacct ggcgcacgat gatgtcggtg
     4141 gtcttttcga tccgttcggg atcgagccgt gccatgcgca gcagttcggc ggccgagctg
     4201 atcggcgcga gcggattgcg cagctcgtgc gcgagcatcg cgagaaattc gtccttgcgc
     4261 cggctcgctt cacgcaactc cagttcggcg cgggcgcgct cgaccgcggc ccagctgcgg
     4321 tcggccacct cgcgcatcag cgcggcctct tcgtcggtcc agtggcgcgg cttgtcgtga
     4381 tgggcgaaca tgaaagccac ggtacgtcca ttgcgctgta ccggcaccgc catcgaggcg
     4441 cgcgcgccga tagcctcatg cgcggaagcg gcggccccgg cgctgcgcgg atcggcggcg
     4501 atgtcgtcga cccgcatcac gcgcccggcg cggatgtcct cgaccagggc gctgccatag
     4561 ttgtcgagcc ggtcccgatg cgcgcgcacg tccggaatcc ggctcgtgtc gttgtagttc
     4621 tcgccgacca ccgcatactc gacgccctcg tccacatcgg catagccgac gcgtggcacg
     4681 tcgagcgccc gggccagcgc atgcgcagac gcatccatca tggcctgcgg gtcgtcggcg
     4741 agattgcgca gcgcgtcgcc gagctcgagc agaacggcct ggcggttggc ggcggcgcgc
     4801 agcgcggctt cgctgcgcgc ctgttccatg gccaagcgga tacgctcgcc gactgccgcc
     4861 acgaccttgt gatcggcatc gctccaatcc cgcggcacga cgtcatacac attcagcacg
     4921 cccaccagcg cacggtcctt tagcagcgga acgagcagtc ccgagcgcag gcccagtgcg
     4981 gcataggcgg cggcgaaatc gcgggtccgg ctgtcggtgc ggacgtcctt gatgcaaatg
     5041 ggccggccgg catgcaggtc ctcgatcagt tgcggcccga agtcgctcag ctttccgcac
     5101 tggccgacca ccgggcacac ccgctcgctg gcccacccgc gcttgatctc gaaggtcccg
     5161 tgcgcatcgt cgatctcgga atagaacacc agcggcgcct cgagacggcg gccgagcaat
     5221 gcggccgccg tttcggtgat ttcgtccact ttcgtcaggt tgcgcaggct gtccacggtt
     5281 tcgagcacca gggcctggcg catgtcttcg cgcatccgtt cggtcgtctc gatgcagatg
     5341 cccagcagtc cgcatacctg gccgctgtca tcgaacaggg gcgagcagga aaaggtgaac
     5401 cagcttcgtt cagtacgtcc atcgcgcgtc aggtcgaccg gcacgtcgcg gcaatagacg
     5461 ggttccccgt tcatcgcatg ttcgatcgcc gagccgagcg caggccaggc ttccggccac
     5521 aggaccggga acgggcggcc cagcgtgccc gggtgtttgt tccccagcaa gggaatgcag
     5581 ccgtcgttgt acaggcagac caggtcccgc ccccagagca gaaacatcgg gaagtccgag
     5641 ttcagcacga tgccgcaggc agtgcgcagc gtcgccggcc aggtctgcgg cgcgcccagc
     5701 ggggtggctg accagtcctg gctgcgcatc agctccgcca tcgcgccggg gcgggcaatg
     5761 aacggcaggg acgtcggatg cagtggctgt gtcatggtga atggtcggcg cgcaacgaag
     5821 caaaagctcc gcgatggtca gatcgccaac gatttgactg tcaattcagg aatggttgcc
     5881 aaatccggac cgcggacatt tgatgtgcat tccgccgggc ggcaaatttc ccgcaagctg
     5941 cctgcagcgc gggttcatgc ggaagggcgg ccattgttgc cgtcggtctc cgggtcgatg
     6001 tgaagttggc aagcggtcgg cgaaatggct accatggcgg cagtcgatct gcgcattgat
     6061 cgggtccggt cggcggcaac tttctccgcg ccatccgctc tattaggatc gcgccatcgg
     6121 gatggcccgg gtgcaatgtt tatcagaggg cgcatgagtc ttgcccaatt catacacgaa
     6181 tatggctatt tcgctgtcgc cataggaagc ttcctcgaag gtgaggcggt cctgctggcc
     6241 ggcagcttcg ccgcctatgc cggccatctc gatctgccgc tggtgctggc gatcgcagcc
     6301 ttggccagct ttctcggcga cctgccgtac ttcttcgccg gccgacgcta cggcagcgcg
     6361 gtgctgcagc gtttcccggc actccacgcg cgcaagggcc gcctcgacaa gttgctgcaa
     6421 cggcatcacg tactgctggt cctgtcgctg cgctttctgt acggcctgcg catcgccggc
     6481 ctgctgacgc tgggcatgag ccgcatgtcg gcttggcgct ttcttgcact cgacttcctg
     6541 ggagccgtga tctggtcgtc gaccgtgtgc gcgaccggct acggggtcgg ccgcctggcg
     6601 caggcgatgt tcggcgacat cagcacaggc gagcagatct cgctgctgct ggcggtgctg
     6661 gtctgcgcgt cgctgctgat catgctggcg cgacgtcgtc tggcgctggc cggcgtggac
     6721 cgcccgccgg gccattgagg ctttttccac gattgcggag ctcggatgca gcgcgaagaa
     6781 ttcctggccc ggcttgccga ggacggcttc aaggaagtcg tgacggtcga gcgcgaggcg
     6841 aacggcgcat gggagctgca tgcccatccg ttcgaggcga aggcgctgat cctgcgcggg
     6901 gaaatccgcc tccgccgcgg cgaggaagag cggacctatc cggccggcac catcttccat
     6961 ctcgccgccg acgagccgca cagcgagtgg tacggcccgc agggcgtcac ctacctggtg
     7021 gggcggcgct gatcagccct tgatgcacac cacctggcgc agcgtatgca cgacctcgac
     7081 cagatcggtc tggtgcgcca tcaccgcgtc gatgtccttg taggcggccg gaatctcatc
     7141 gatcacgccg ccgtccttcc tgcattcgat gccgcgcgtc tgctcggcca ggtcgaagcg
     7201 attgaagcgg cgcttggcct cggtgcggct catgcgccgg cccgcgccgt gcgagcagga
     7261 gcagaacgaa tccggattgc ccttgccgcg caccacgtag ctgcgcgcgc ccatgctgcc
     7321 ggggatgatg cccagttcgc cctcgcgcgc ggaaatcgcc cccttcctgg tgacgaacag
     7381 ccgttcgccg ccgtgtgtct cccgcgagac gtagttgtga tggcaattga ttgcttcgcc
     7441 gtcgagcctg aacggcggca gctgctggat cagcacgtcg accacgcggc gcatcatctc
     7501 gcgccggttc accagcgcat agtcctgcgc ccattcgacc gcttcgacgt agtcgtcgaa
     7561 cagctccgag ccttcccgga agtaggagag atccctgtcc ggcagccgga tctggtggcg
     7621 ttccatctcc ttgcgcgcgg cggcgatgaa gtaacggccg atcgcgttgc cgatgccgcg
     7681 cgagcccgag tgcagcatga tccacacgcg ctgttcctcg tccaggcaga gctcgatgaa
     7741 atggttgccg ccgcccagcg tgccgagctg gcagatccag gtctgctcga acttctgctg
     7801 catctttgcg atgccgcggt gccgtccgat gatgcggtcg agccggtccg acagcgggcg
     7861 cgccgcgcgc gcatgggcgg cgccgcgcac cttgttccac tcgtgctggg cgaagccgac
     7921 cggcaccgcg ccctcgatcg cgccgcgcag gcgcgccaga ctgtcgggca ggtcgcttgc
     7981 ggtcagcgtg gtgcgcaccg cgttcatccc gcagccgatg tcgaccccga cggcggccgg
     8041 aatgaccgtc gagcgggtcg gaatcacgct gccgacggtc gcgccgatgc cggcatgcac
     8101 atccggcatc gccgccacgt gcggatgcac gatcggcaac tgcgcgatgt tctgcag
//
LOCUS       DATLGF010000346         5686 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_213255, whole genome shotgun sequence.
ACCESSION   DATLGF010000346 DATLGF010000000
VERSION     DATLGF010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213255"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VM406_10605"
     CDS             complement(<1..527)
                     /locus_tag="VM406_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HVL76455.1"
                     /translation="MNRPTLRFRPRLLDALQDYDRKRLFQDMNAGITVGVVALPLAMA
                     FAIASGVRPEAGIFTAIIAGFLIAALGGSKVQIGGPAGAFIIVVYGIIETYGLGGLLV
                     STVLAGIILFAMGLLRLGFLIRYIPVPVVIGFTNGIAILIALSQISDFFGLAIDRMPS
                     DFFAQLRTLAAHAHTV"
     gene            complement(547..1029)
                     /locus_tag="VM406_10610"
     CDS             complement(547..1029)
                     /locus_tag="VM406_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL76456.1"
                     /translation="MNVTVREATLEDAQLIAELTRACWAKRVALSSSGHRETAVRVLQ
                     HLQEGGGFVMLDGDEPIGSVRWLPLEGQGDIWEILRMGVLPRCSGRHLSERLLEAVIH
                     RALDAGVDELRLAVRSDQERLVDLYSTYGFVLAPELEYTHANPLEPPPTVMRRALDHH
                     "
     gene            complement(1076..3199)
                     /gene="metG"
                     /locus_tag="VM406_10615"
     CDS             complement(1076..3199)
                     /gene="metG"
                     /locus_tag="VM406_10615"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HVL76457.1"
                     /translation="MSTTPSRKLFVTTALPYANGAFHIGHIMEYIQADIWVRFQRMQG
                     HDVHFVGADDAHGTPIMIAAEKAGVTPQEFVAKIAAGRQQYLDGFHISFDNWHSTDSP
                     ENTELSQGIYLRLREAGLIATRVVERFFDPVKGMFLADRNIKGECPRCHAKDQYGDNC
                     EVCGAAYEPTELINPYSALTGATPVQKPSEQYFFKLSDPRCIAFLQEWLNSPGRLQPE
                     MVNKVSEWLGNDGRDLADWDISRDAPYFGIPIPDAPGKYFYVWLDAPVGYLASLKNYC
                     GKKGIDYDALLHDPATEQVHFIGKDIVSFHLLFWPTMLKFSGHPIIEKLKVNVHGHLT
                     VNGEKMSKSRGTGISPLRYLDIKMNPEWLRYYIATKLNSKVEDIDFNAEDFIAKVNSD
                     LIGKYVNICSRAAGFISKRFDGKLSNPVAEGSSTNRFWHQLAGEEAAIREAYEAREYG
                     KAIRHIMALADEVNQYVDAKKPWELAKDPANNKALQDVCSDLLLAFHRLSVFLAPVLP
                     SVARKVAALFGLERDLNWKDLESYPAQIGTFSHLMTRVEAKMLEDLFEKAEVSTAGTV
                     AGSSNPPPSQPFPSKGEGVGGEAIEPIAPEIKIDDFSRIDLRIAKIVNCEHVEGSDKL
                     LRLTLDVGEGRMRNVFSGIKSSYKPEDLIGKLTVMVANLAPRKMKFGVSEGMVLAASS
                     ADEKTNPGIYVLNPWPGAEPGMRVR"
     gene            3302..4390
                     /gene="apbC"
                     /locus_tag="VM406_10620"
     CDS             3302..4390
                     /gene="apbC"
                     /locus_tag="VM406_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HVL76458.1"
                     /translation="MTLTTDAVRTALAAVTDPNTGKDLVAGKSAKNIRIEGADVHVDV
                     ELGYPAKSQIEPIGAAVRAVLQTLPGVGRIEVNVYSKIVPHAVQRGVPLLPNVRNIIA
                     VASGKGGVGKSTTAANLALALAAEGARVGLLDADIYGPSQPMMMGIKGQPESKDGKTM
                     EPLENHGVQVASIGFMIDPDQPMVWRGPMVTQALQQLLQQTNWRDLDYLLVDMPPGTG
                     DIQLTLSQKVPVTGAVIVTTPQDIALLDARKGLKMFEKVGIPILGIVENMSVHICSNC
                     GHAEPIFGAGGGEKMGAEYGAEFLGALPLLLSIREQTDSGTPTVVADPEGQAAQIYKQ
                     IARRIAVKVAEKAKDMTSKFPSIVIKND"
     gene            4598..>5686
                     /gene="gltX"
                     /locus_tag="VM406_10625"
     CDS             4598..>5686
                     /gene="gltX"
                     /locus_tag="VM406_10625"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HVL76459.1"
                     /translation="MTVRTPVRTRFAPSPTGYLHLGGARTALFSWAYARHFGGTFILR
                     IEDTDLERSTPEAVQAILDGMNWLGLQHDEGPFYQMQRMDRYREVVAQMLEAGTAYYC
                     YCTPEEVEAMRERMRAAGEKPRYDGTWRPEPGKTLPAVPEGRKPVVRFKNPLDGDVTW
                     NDVVKGPITISNRELDDLVIARPDGTPTYNFCVAVDDWDMRITHVIRGDDHVNNTPRQ
                     INILRALGAPLPEYGHVPMILGADGEKLSKRHGAVSVMEYPAQGYLPEAMLNYLARLG
                     WSHGDDEIFSIEQFVQWFDLDHLSKSPAQFNPEKLAWLNNHYIKQADNARLAGLVTPF
                     IEELGGKVEGGPMLADVVALVKDRANTLR"
BASE COUNT         1043 a         1767 c         1885 g          991 t
ORIGIN      
        1 accgtgtgcg cgtgggcggc gagcgtgcgc agctgcgcga agaaatccga cggcatgcgg
       61 tcgatcgcaa ggccgaagaa gtcgctgatc tgggacagcg cgatcaggat tgcaatgccg
      121 ttggtaaagc cgatcacgac cggcaccggg atgtagcgga tcaggaagcc gagccgcagc
      181 agccccatcg caaacaggat gatgccggcc aacaccgtgg agaccagcag cccccccagc
      241 ccataggtct cgataatgcc gtagacgacg atgatgaagg cgccggccgg cccgcctatc
      301 tgcaccttcg agccgcccag cgcggcaatc agaaagccgg cgatgatcgc ggtgaagatg
      361 ccggcttccg gccgcacccc ggaggcgatt gcgaacgcca tcgccagcgg cagcgccacc
      421 accccgaccg tgatccccgc gttcatatcc tggaacaggc gtttgcgatc gtagtcctgc
      481 agcgcgtcga gcaggcgcgg acggaagcgg agggtggggc ggttcatcgg cgtgtcggtc
      541 tgcgtgctag tgatgatcca aggcccgacg catcaccgtg ggcggcggct cgagcggatt
      601 ggcgtgggtg tattcgagtt cgggcgcgag cacgaaaccg tacgtggagt agaggtcgac
      661 caggcgttcc tggtcgctac gcacggcgag gcgcagttcg tcgacccccg cgtcgagcgc
      721 gcggtggatc acggcctcga gcaggcgttc ggagagatgg cggcctgagc aacgtggcag
      781 cacgcccatg cgcaggattt cccatatgtc gccctggcct tcaagcggca gccagcgtac
      841 cgaaccaatc ggttcgtcgc cgtcgagcat cacgaatccg ccgccttcct gcaggtgctg
      901 caagacgcgc accgcggttt cgcgatggcc gctggaactg agcgccacac gcttggccca
      961 acaggcgcgg gtgagttccg cgatcagctg ggcgtcctcc agggtggctt cacgcacggt
     1021 cacgttcatg cgcggccacc gatggaagaa ttgcctgggg cagggtcggg catgatcagc
     1081 gcacgcgcat gccgggctcc gcgcccggcc acgggttgag cacgtagatt cccggattgg
     1141 tcttctcgtc ggcggacgag gcggccagca ccatgccctc ggagacgccg aacttcatct
     1201 tgcgcggcgc gaggttggca accatcacgg tgagtttgcc gatcaggtcc tccggcttgt
     1261 aagaggactt gatgccggag aacacgttgc gcatgcggcc ttcgccgacg tccagcgtca
     1321 ggcgcagcag cttgtcggag ccttcgacgt gttcgcagtt gacgatcttc gcaatgcgca
     1381 agtcgatcct ggagaaatcg tcaatcttga tctccggggc gatcggttcg atcgcctcgc
     1441 cgcctactcc ttcccccttt gaggggaaag gttgggatgg gggtggattg ctcgaacccg
     1501 cgacggtccc tgcagtagaa acctccgcct tctcaaacaa atcctccagc atcttcgcct
     1561 ctacccgcgt catcagatgc gagaaagtgc cgatctgcgc cggatagctc tccagatcct
     1621 tccagttcag atcgcgctcc agcccgaaca gcgccgcaac cttgcgggct acggacggca
     1681 gcaccggcgc gaggaacacc gacagccggt ggaaggccag gagcaggtcg ctgcagacgt
     1741 cctgcagcgc cttgttgttg gccggatcct tggccagttc ccacggcttc ttcgcatcca
     1801 cgtactggtt gacctcgtcg gccagcgcca tgatgtggcg gattgccttg ccgtactcgc
     1861 gcgcctcgta ggcttcgcgg atggccgctt cctctccggc aagctggtgc cagaagcggt
     1921 tggtgctgct gccttcggcc acagggttcg acagcttgcc gtcgaagcgc ttgctgatga
     1981 agccggccgc acggctgcag atgttcacgt acttgccgat caggtcggaa ttgaccttgg
     2041 cgatgaagtc ttccgcgttg aagtcgatgt cctcgacctt ggaattgagc ttggtcgcga
     2101 tgtagtagcg cagccactcc gggttcatct tgatgtcgag gtagcgcagc ggcgagatgc
     2161 cggtgccgcg cgacttgctc atcttctcgc cgttgacggt caggtggccg tgcacgttga
     2221 ccttgagctt ttcgatgatc ggatgccccg agaacttgag catcgtcggc cagaaaagca
     2281 ggtggaagga gacgatgtcc ttgccgatga agtgcacctg ttcggtggcg gggtcatgca
     2341 gcagcgcatc gtagtcgatg cccttcttgc cgcaatagtt cttcaggctg gcaagatagc
     2401 cgaccggcgc gtccagccac acgtagaagt acttgccggg tgcgtccggg atcggaatcc
     2461 cgaagtaagg cgcgtcgcgc gagatgtccc agtcggcgag gtcgcggccg tcgttgccga
     2521 gccactcgga caccttgttc accatctcgg gctgcaggcg tccgggcgaa ttcagccatt
     2581 cctgcaggaa ggcaatgcag cgcgggtcgg acagcttgaa gaaatactgc tcggacggct
     2641 tttgcaccgg ggtggcgccg gtcagcgccg aatacggatt gatcagctcg gtcggctcgt
     2701 aggcggcgcc gcagacctcg cagttgtcgc cgtactggtc cttcgcatgg cagcgcgggc
     2761 actcgccctt gatgttgcgg tcagccagga acatgccctt gaccggatcg aagaagcgct
     2821 cgaccacgcg cgtggcgatc agcccggctt cgcgcaggcg caggtagatg ccttgcgaca
     2881 gctcggtgtt ttcgggagag tcggtcgagt gccagttgtc gaaggagatg tggaagccgt
     2941 ccaggtactg ctggcgtccg gcggcgatct tcgcgacgaa ttcctgcggt gtcacgccgg
     3001 ccttctcggc ggcgatcatg atcggcgtgc catgtgcgtc gtccgcgccg acgaagtgca
     3061 cgtcatggcc ttgcatgcgc tggaagcgta cccagatgtc ggcctggatg tattccatga
     3121 tgtggccgat atggaaggcg ccgttggcgt agggcagggc ggtggtgacg aacagcttgc
     3181 gggacggggt ggtgctcatt tggcaatgcg cgtgcggaaa taaaactgcc attctagcag
     3241 tccactccgg tggggcaggg ccgcctggtt tagacttgca gccgaaaatc taggagaaat
     3301 catgactttg acaacggacg cggtacgcac tgcgctcgcg gcagtgacgg acccgaacac
     3361 gggcaaggac ctggtcgccg ggaagtcggc gaaaaacatc cgcatcgagg gagccgacgt
     3421 ccacgtcgac gtcgagctcg gttacccggc aaagagccag atcgaaccga tcggcgcggc
     3481 ggtgcgggcg gtgctgcaga cgctgccggg cgtgggcagg atagaagtca acgtctattc
     3541 gaagatcgtg ccgcatgcgg tgcagcgcgg cgtgccgctg ctgccgaacg tgcgcaacat
     3601 catcgcggtc gcctcgggca agggcggtgt cggcaagtcg accaccgccg ccaacctggc
     3661 gctggcgctg gccgcagaag gcgcgcgggt gggcctgctg gacgccgaca tctacggccc
     3721 gtcgcagccg atgatgatgg gcatcaaggg ccagccggag tcgaaggacg gcaagacgat
     3781 ggagccgctg gagaaccacg gcgtgcaggt ggcctcgatc ggcttcatga tcgatcccga
     3841 ccagccgatg gtctggcgcg gcccgatggt gacgcaggcg ctgcagcaac tgctgcagca
     3901 gaccaactgg cgcgatctcg actacctgct ggtggacatg ccgccgggca ccggcgacat
     3961 ccagttgacg ctgtcgcaga aggtgccggt gaccggggcg gtgatcgtca ccacaccgca
     4021 ggacattgcg ctgctcgatg cgcgcaaggg gctgaagatg ttcgagaagg tcggcattcc
     4081 gatcctcggc atcgtcgaga acatgagcgt gcacatctgc tcgaactgcg gccatgccga
     4141 gcctatcttc ggtgcaggag gcggcgaaaa gatgggcgcc gaatacggcg cggaattcct
     4201 cggggccctg ccgctgttgc tgtcgatccg cgagcagacc gactccggca cgcccacggt
     4261 cgtcgccgac ccggaaggcc aggccgcgca gatctacaag cagatcgcgc gccgcatcgc
     4321 ggtgaaggtc gccgaaaagg caaaggacat gacgagcaag tttccgagta tcgtgatcaa
     4381 gaacgattga gctttgccgg cacggtaaaa tagccggttt cccggcgacg gccttcaagc
     4441 tgtcgttccc gcgcaggcgg gaacccagtc cggacgtggc agcggcatcg cggcatctcc
     4501 cgggctcccg cctgcgcagg tgcgacatgc agatgttgcc gggcttccgc gcacttttca
     4561 gaacaccaga tcattcccgt cagggcattt ttaactcatg accgtccgca cccctgttcg
     4621 tacaagattc gcccctagcc ccaccggcta cctccacctc ggcggcgcgc gcaccgcgct
     4681 gttctcctgg gcctatgcgc gccactttgg aggcaccttc atcctgcgca tcgaagacac
     4741 cgacctcgag cgcagcacgc cggaagcggt gcaggcgatc ctcgacggca tgaattggct
     4801 tggcctgcaa cacgacgaag gcccgttcta tcagatgcag cggatggacc gctatcgcga
     4861 ggtcgtcgcc cagatgctcg aggccgggac cgcctattac tgctactgca cgccggagga
     4921 agtggaggcg atgcgcgagc gcatgcgcgc ggcgggcgaa aagccgcgct acgacggcac
     4981 ctggcgcccg gagccgggca agacgctgcc cgcggtcccc gaaggccgca agccggtggt
     5041 gcgcttcaag aacccgctcg acggtgatgt gacctggaac gatgtagtca aggggccgat
     5101 cacgatctcc aaccgcgaac tcgacgacct cgtgatcgcg cgcccggacg gcacgccgac
     5161 ctacaacttc tgcgtcgccg tggatgactg ggacatgcgc atcacccacg tgattcgcgg
     5221 cgacgatcac gtgaacaaca cgccgcgcca gatcaacatc ctgcgtgcgc tcggtgcgcc
     5281 gctgccggaa tacggccatg tgccgatgat cctcggcgcc gacggcgaaa agctgtccaa
     5341 gcgccacggt gcggtgagcg tgatggagta tccggcccag ggctatctgc cggaggccat
     5401 gctcaactac ctcgcgcgcc tgggctggag tcacggcgat gacgagatct tctcgatcga
     5461 gcagttcgtg cagtggttcg atcttgatca cctgtcgaag tcgcccgcgc agttcaatcc
     5521 ggaaaagctg gcctggctga acaaccacta catcaagcag gccgacaacg cgcgcctggc
     5581 cggcctcgtg acgcccttca ttgaagagct gggcggcaag gtcgaaggtg gcccgatgct
     5641 ggccgacgtg gttgcgctgg tgaaggaccg cgccaacacg ctgcgt
//
LOCUS       DATLGF010000347         8110 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_30482,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000347 DATLGF010000000
VERSION     DATLGF010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 8110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8110
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30482"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /gene="ampD"
                     /locus_tag="VM406_10630"
     CDS             <1..484
                     /gene="ampD"
                     /locus_tag="VM406_10630"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HVL76460.1"
                     /translation="SLPPGQFGGAHIEALFTNSLDCDAHPYFDRLRTLKVSAHFLIAR
                     SGEVLQFVPTVARAWHAGASNFCGRERCNDFSIGIELEGCDDLPFEPIQYAVLAELTI
                     ALRARHPLAGVAGHEHIAPGRKTDPGPCFDWDLYRKSLIQMDAALRDLSALRFPGAIL
                     "
     gene            805..3720
                     /locus_tag="VM406_10635"
     CDS             805..3720
                     /locus_tag="VM406_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HVL76461.1"
                     /translation="MQPTQELSVKSTTNPGKASTATGTGTQVSAPHGALADYKIIRRN
                     GAVVGFEPSKISIAMTKAFLAVNGGQGAASARVRELVDQLTQGVVGALVRRQPAGGTF
                     HIEDVQDQVELALMRSGEHDVARAYVLYRAKRMEERAQQKAAQQVATPQLHVTEGGQS
                     RPLDIEQVRTLISAACVGLEKHVDAEAILSETLKNLYDGVPVEELHKSAILAARALME
                     KDPAYSRVTARLLMHTIRKEVFGQEVAQADAQQHYIDYFPKFIRKGIEADLLDPALAQ
                     FDLQQLAEALKPERDLQFGYLGLQTLYDRYFLHIQGTRIEMPQAFYMRVAMGLALNEI
                     NREARAIEFYNLLSSFDFMSSTPTLFNSGTQRSQLSSCYLTTVSDDLEGIYEAIKENA
                     LLAKFAGGLGNDWTPVRALGAHIKGTNGKSQGVVPFLKVVNDTAVAVNQGGKRKGAVC
                     AYLETWHMDIEEFLDLRKNTGDDRRRTHDMNTANWIPDLFMKRVMEGGEWTLFSPSDV
                     PDLHDKYGRAFEEAYTAYEAKAARGELKLYKKVQALDLWRKMLSMLFETGHPWITFKD
                     PCNIRSPQQHVGVVHSSNLCTEITLNTNDSEIAVCNLGSVNLPAHMKDGKLDHAKLQR
                     TIRTAMRMLDNVIDINYYAVKKARDSNLRHRPVGMGIMGFQDCLHMMRVPYASMDAVE
                     FADRSMEAVCYYAYWASTELAEERGRYSSYQGSLWDRGILPQDSLKLLAEERGGYLEV
                     DTSETMDWASLRARIKEHGMRNSNCVAIAPTATISNIIDVSACIEPTYQNLYVKSNLS
                     GEFTEINENLVRDLKARGLWDEVMVADLKYFDGSLAKIDRIPQDLRDLYATAFEVEPK
                     WLVEAASRRQKWIDQAQSLNIYMAGASGKKLDETYKLAWLRGLKTTYYLRTIGATHAE
                     KSTSKAGALNAVSAHAAPAAATSAAPAAPAAATDELAGPACMLRPGDPGFEECEACQ"
     gene            3799..4968
                     /locus_tag="VM406_10640"
     CDS             3799..4968
                     /locus_tag="VM406_10640"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HVL76462.1"
                     /translation="MLSWDDEQSPAAKPAPMPQQALAPAVGLNAGLSNTALDPALVGR
                     AAADAESRRVNAADKRIINGQTDVNQLVPFKYKWAWDKYLAGCANHWMPQEINMQRDI
                     ELWKNPNGLTEDERRIVKRNLGFFVTADSLAANNIVLGTYRHITAPECRQYLLRQAFE
                     EAIHTHAYQYIVESLGLDEGEIFNAYQEIESIRDKDEFLIPFIDVLTNPEFKTGTVEA
                     DQTLLRSLIVFACLMEGLFFYVGFTQILALGRQNKMMGAAEQYQYILRDESMHCNFGI
                     DLINTIKLENPHLWTPEFREEIKALFLRAVELEYRYAEDTMPRGVLGLNAPMFKGYLR
                     FIANRRAQQIGLDPLFPQEENPFPWMSEMIDLKKERNFFETRVIEYQTGGALSWE"
     gene            complement(5138..5491)
                     /locus_tag="VM406_10645"
     CDS             complement(5138..5491)
                     /locus_tag="VM406_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76463.1"
                     /translation="MNEIKLRGLKPTRRIAMPPHGLFIGTSKLLLALMNTAPAELLHH
                     LMHAALQPHPVAPLLLLVTTTLAMTEGLKSRAPCAPRRPQPEKTPAQCRRRNKVYEKP
                     TRFSKKKPPRPTASS"
     gene            5507..5746
                     /locus_tag="VM406_10650"
     CDS             5507..5746
                     /locus_tag="VM406_10650"
                     /inference="COORDINATES: protein motif:HMM:NF019028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1-like repetitive region-containing
                     protein"
                     /protein_id="HVL76464.1"
                     /translation="MATAKKTAAKKAAAKKPAAKKAAADKPVAKKAAAPKKAAAPKKA
                     AAPKKAAAPKKAAAPAAKTVLNPAAAWPFPTGTRP"
     gene            5843..6385
                     /locus_tag="VM406_10655"
     CDS             5843..6385
                     /locus_tag="VM406_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HVL76465.1"
                     /translation="MLGSILTLIIDTIAVILGGALLLRFWMQVVRVRPPNQIGQFIYK
                     LSDWLVLPLRRMLPGSGGYDWASLIGAILVIMAATALKMLLFTGITMQFLVLGTLLRL
                     SEWIAYGFMALIIIEAIFSWVNPHAPLAPFIRALNEPLLRPLRRVIPLLGGIDLSPLV
                     ALIGLRIALMIVTDLLLRLA"
     gene            complement(6514..7452)
                     /locus_tag="VM406_10660"
     CDS             complement(6514..7452)
                     /locus_tag="VM406_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HVL76466.1"
                     /translation="MTTTSLICGSLAFDTIMLFRGRFSEALLADQLHKINVAFLVPEV
                     RREFGGCAGNIAYNLRLLEGDPLIMATIGSDGGPYLERLEQLRISPRCVRTIETSYTA
                     QAYITTDEGGNQITAFHPGAMSFSHQNRVADAGAVKLAIIAPDGRDGMLQHARDCAEL
                     GIPFIFDPGQGLPMFNGKELEHLVELATYVAVNDYEAELLMERTGLSLEALAERVTGL
                     IVTRGERGSEIYASGRRIDIPCVPADGVVDPTGCGDAFRAGLMYGLTNGMDWETTGRL
                     ASLMGSIKIAHQGGQNHAPARAEIETRFAQAFGYRF"
     gene            complement(7464..>8110)
                     /locus_tag="VM406_10665"
     CDS             complement(7464..>8110)
                     /locus_tag="VM406_10665"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HVL76467.1"
                     /translation="SHHTAPEPAATEGPDPLDQAIEDLQRRPLRGAGTAVRDEDEPSF
                     VRQGRRRQRMGRLATVTMSAGSVLLLVALIAQSAYVFRDQLAARFPQTAPWLHEACAY
                     LGCQVGLPAQIEAVAIESSELQTLAPNTSTLGLTVLLRNHGTVAQSWPYLELTLNDNA
                     GKVLARRVFAPSEYLPGGSNPRTGFAARSEQPVRLFFEPEGIQPSGYRVYVFYP"
BASE COUNT         1588 a         2526 c         2554 g         1442 t
ORIGIN      
        1 cagcctgccg ccggggcagt tcggtggcgc gcatatcgag gccttgttca ccaactccct
       61 cgattgcgac gcgcatccgt acttcgatag gctgcgcacg ctgaaggtat cggcgcactt
      121 cctgatcgcg cgcagcggcg aggtcctgca attcgtgccg accgtcgcgc gcgcatggca
      181 cgccggcgcg tcgaacttct gcggacgcga gcgctgcaat gacttctcga tcggcatcga
      241 actcgaaggc tgcgacgacc tgccgttcga accgatccag tacgccgtac ttgcagaact
      301 caccatcgcg ttgcgcgcac gccatccgct ggccggtgtc gccggacatg aacatattgc
      361 gccaggccgc aagaccgacc ccgggccctg cttcgattgg gacctttatc gcaagtcctt
      421 gattcaaatg gatgctgcgc tgcgagacct cagcgcgctg cgctttcccg gcgccatcct
      481 ctgaaaaagt caaggtcgca gcgcgggaat tgattgccca agcggcaaaa aaacctgttg
      541 cgcaggcggg atgcgggcac tagaattagt ttcgatcgca gtcgtgacca ctatatattg
      601 tggttgtgtg atttcactta ggctgttctg gttgtacacg attcagatgg gcaggccgaa
      661 cagcactgcc ttttcaggac gacgcatgca tgtgcatgct gtctgtcccg cgcggaaaaa
      721 tcccggcctg gcatggccgg actttcgttc aaagacaacc cctgcggccc tgcgccgcga
      781 agcgtaacca tccaaggagg cacgatgcaa cctacgcaag aactttccgt caagtcgacg
      841 accaatcccg gtaaggcctc caccgcgacc ggtaccggca cccaggtctc ggccccgcac
      901 ggggcgctcg ccgactacaa gatcatccgc cgcaatggcg cggtagtcgg cttcgagccg
      961 tcgaagatct cgatcgcgat gaccaaggcc ttcctcgccg tcaatggcgg ccagggcgcc
     1021 gcttccgcgc gcgtgcgcga gctggtcgat cagctgaccc aaggcgtggt cggggctctg
     1081 gtgcgccgcc agccggccgg cggcaccttc catatcgaag acgtgcagga ccaggtcgag
     1141 ctcgcgctga tgcgctccgg cgagcatgac gtcgcacgtg cctacgtgct ctaccgcgcc
     1201 aagcgcatgg aggaacgcgc tcagcagaag gccgcccagc aggtcgcgac cccgcaactg
     1261 catgtgaccg aaggcggcca gagccgtccg ctcgacatcg agcaggtgcg cacgctgatc
     1321 tccgccgcct gcgtcggcct tgagaagcac gttgatgccg aggcgatcct gtccgagacg
     1381 ttgaagaatc tctacgacgg cgtgccggtc gaagagctgc acaagtccgc catcctggct
     1441 gcccgcgcgc tgatggaaaa ggacccggcc tacagccgcg tcaccgcgcg cctgctgatg
     1501 catacgatcc gcaaggaagt gttcggccag gaagtggccc aggccgacgc gcagcaacac
     1561 tatattgatt acttcccgaa gttcatcagg aagggtatcg aggccgacct gctcgacccg
     1621 gcgctggccc agttcgacct gcaacagctg gccgaggccc tgaagcccga gcgcgacctg
     1681 cagttcggct acctcggcct gcagacgctg tatgaccgtt actttctgca tatccagggc
     1741 actcgcatcg agatgccgca ggcgttctac atgcgcgtgg cgatgggcct tgcgctcaac
     1801 gagatcaacc gcgaagcgcg cgcgatcgag ttctacaacc tgctgtcgtc gttcgatttc
     1861 atgagctcga cgccgacgct gttcaattcc ggcacccagc gctcgcagct ttcctcctgc
     1921 tatctgacca ccgtgtccga cgacctcgaa ggcatctacg aggcgatcaa ggagaatgcg
     1981 ctgctggcca agttcgccgg cggactcggc aacgactgga ccccggtgcg tgcgctcggc
     2041 gcccacatca agggtaccaa tggcaagtcg cagggcgtgg tgccgttcct gaaggtggtc
     2101 aacgacaccg cggtcgcggt gaaccagggc ggcaagcgca agggcgcggt ctgtgcctac
     2161 ctggaaacct ggcacatgga tatcgaggaa ttcctcgacc tgcgcaagaa caccggcgac
     2221 gaccgccgtc gcacccacga catgaacacc gcgaactgga ttcccgacct gttcatgaag
     2281 cgcgtgatgg aaggtggcga gtggacgctg ttctccccgt ccgacgtgcc ggacctgcac
     2341 gacaagtacg gccgcgcctt cgaagaggcc tacaccgcct atgaagcgaa ggctgcgcgc
     2401 ggcgaactga agctctataa gaaggtgcag gcgctcgatc tgtggcgcaa gatgctgtcg
     2461 atgctgttcg agaccggcca tccgtggatc accttcaagg atccgtgcaa catccgctcg
     2521 ccgcagcagc acgtgggcgt cgtgcacagc tcgaacctgt gcaccgagat cacgctcaac
     2581 accaacgatt ccgaaatcgc ggtctgcaat ctgggctcgg tcaacctgcc ggcccacatg
     2641 aaggatggaa agctcgatca cgcgaagctg cagcggacga tccgcaccgc gatgcgcatg
     2701 ctcgacaatg tgatcgacat caactactac gcggtgaaga aggcgcgcga ttccaacctg
     2761 cgccaccgtc cggtcggcat gggcatcatg ggcttccagg actgcctgca catgatgcgc
     2821 gtaccgtacg catcgatgga cgcggtcgag ttcgccgacc gttcgatgga ggcggtgtgc
     2881 tactacgcgt actgggcatc gaccgagctg gccgaggagc gcggtcgcta cagctcctac
     2941 cagggttcgc tgtgggaccg cggcatcctg ccgcaggact cgctgaagct gctggccgag
     3001 gaacgcggcg ggtatctcga ggtcgacacc tccgagacga tggactgggc gtccctgcgt
     3061 gcgcgcatca aggagcacgg catgcgcaac tccaactgcg tggcaatcgc accgaccgcg
     3121 acgatctcga acatcatcga cgtgtccgcc tgcatcgagc cgacctacca gaacctgtat
     3181 gtgaagtcga acctgtccgg cgaatttacc gagatcaacg agaacctggt gcgcgacctg
     3241 aaggcgcgcg ggctgtggga cgaggtgatg gttgccgacc tgaagtactt cgacggcagc
     3301 ctcgcgaaaa tcgaccgcat tccgcaggat ctgcgcgacc tgtatgcgac cgccttcgag
     3361 gtcgagccga agtggctggt ggaggccgcg tcgcgccgcc agaagtggat cgaccaggcg
     3421 caatcgctca atatctatat ggcgggtgcc tccggcaaga agctcgacga gacctacaag
     3481 ctggcatggc tgcgcggcct gaagaccacg tattacctgc gcacgatcgg cgcgacccac
     3541 gccgagaagt cgacctccaa ggccggcgcg ctgaacgcgg tgtcggccca tgcggcaccc
     3601 gctgccgcga cgtcggccgc gccggccgcg cccgccgcag ccactgacga gctcgcgggt
     3661 ccggcatgca tgctgcgtcc gggcgacccg ggcttcgaag agtgcgaagc ctgccagtaa
     3721 aaggaactcg agattgcgcg gcatggatgc cgcgcaatcc atccgcaccg tgaacacata
     3781 tctgtaagga attccgccat gctcagttgg gacgatgaac aaagccccgc agcaaagccc
     3841 gcccccatgc cgcagcaggc cctggcgccg gctgtcggcc tgaacgcagg cctctccaat
     3901 accgcgctcg atccggcgct ggtcggccgt gctgccgcag acgccgagtc gcgtcgcgtc
     3961 aacgcggcgg acaagcgcat catcaacggc cagaccgacg tcaaccagtt ggtcccgttc
     4021 aagtacaagt gggcctggga caagtacctg gccggctgcg cgaaccactg gatgccgcag
     4081 gaaatcaaca tgcagcgcga catcgagctg tggaaaaacc cgaacggcct gaccgaggac
     4141 gagcggcgca tcgtcaagcg caacctcggg ttcttcgtga ccgccgattc gctggctgca
     4201 aacaacatcg tgctcggcac ctaccgccat atcacggcgc cggagtgccg ccagtacctg
     4261 ctgcgccagg ccttcgaaga ggcaatccac acccacgcat accagtacat cgtcgagtcg
     4321 ctcggtctcg acgaaggcga gatcttcaac gcctatcaag agatcgaatc aattcgcgac
     4381 aaggacgagt tcctgatccc gttcatcgac gtgctgacca atcccgagtt caagaccggc
     4441 acggtggaag cggaccagac gctgttgcgc tcgctgatcg tgttcgcctg tctgatggag
     4501 ggactgttct tctatgtcgg cttcacgcag atcctcgcgc tcggacggca gaacaagatg
     4561 atgggcgcgg ctgagcagta tcaatacatc ctgcgcgacg agtccatgca ctgcaacttc
     4621 gggatcgatc tgatcaacac gatcaagctc gagaacccgc atctgtggac tcccgagttc
     4681 cgcgaagaga tcaaagcgtt gtttttgcgc gcggtagagt tggaatatcg ctacgctgag
     4741 gatacaatgc cgcgaggtgt gctgggcttg aatgcgccga tgttcaaggg gtacctgcga
     4801 ttcatcgcaa accgtcgtgc gcagcagatc ggtctcgatc cgttgttccc tcaggaggag
     4861 aatcccttcc cgtggatgag cgagatgatc gatctgaaga aagaacgcaa cttcttcgag
     4921 acacgcgtga tcgaatatca gaccggtggc gcgctgagct gggagtagga ttgagatcgc
     4981 tgccatcagc gcaccgggga gaggcaaaac gaagttggta ctgaaaacgg agtagcggga
     5041 gaaccgacca agtcgaacca ctcactgaat gcacgaaagg cagcaaccta tttgatgaac
     5101 agtagacgaa cgattcgctc ttctgtggtg gggccgctta gctcgacgct gtcgggcgag
     5161 gcggcttttt ctttgaaaaa cgtgttggtt tttcataaac cttgttccga cgccggcatt
     5221 gcgccggcgt tttttccggt tgcggacgcc gcggcgcgca gggcgcgcgc gacttgagac
     5281 cctccgtcat cgccagcgtg gtggtgacaa gcaacaagag aggcgcaacc ggatgaggct
     5341 gaagtgctgc atgcatgagg tgatgcagca gttcagctgg tgccgtattc attagcgcaa
     5401 gcagaagctt gcttgtgccg atgaataatc cgtgcggagg catcgcgatg cgccgcgtgg
     5461 gtttcaaacc tcgtagtttg atttcattca cgtgaaggag aaacacatgg caacagcaaa
     5521 gaaaacggct gccaagaagg ccgcggctaa gaagcccgcc gccaagaagg ccgccgccga
     5581 caagccggta gcgaagaagg ccgcagcacc gaagaaggca gccgctccga agaaggcggc
     5641 cgctccgaag aaggcagccg ctcccaagaa ggccgcagcg cctgcagcca agactgtgct
     5701 gaacccggca gcggcatggc cgttcccgac cggcacccgt ccgtaatcga gagagccgct
     5761 ttggtagcct gaatcagttt cgggcacaat gcccgttgcg tggcttggcg ctacgcaacg
     5821 ggtttttcat ttgggagaca acatgctggg cagcatcctg acgctgatca tcgacaccat
     5881 cgccgtgatt ctcggcggcg ccttgctgct gcgcttctgg atgcaggtgg tgcgcgtgcg
     5941 tccgccgaac cagatcgggc agttcatcta caagctgtcg gactggctcg tgctgccgtt
     6001 gcgccgcatg ctgccgggct ccggcggcta tgactgggcg agcctgatcg gcgcaatcct
     6061 ggtgatcatg gccgccacag cactgaagat gctgttgttt accgggatca cgatgcagtt
     6121 cctggtgctc ggtacgctgc tgcgcctgtc tgaatggatc gcgtatgggt tcatggcgct
     6181 gatcatcatc gaagcgatct tcagctgggt gaacccgcat gcgccgctcg ccccgttcat
     6241 cagagcactc aatgagccgt tgctgcggcc actgcgccgc gtcatacctt tgctcggcgg
     6301 gatcgatctg tcgccgctgg tggcgctgat cggcctgcgc atcgcattga tgatcgtcac
     6361 cgacctgctg ctgcgcctgg cgtgaacgct ggcgcactca gcgtcaagcc tgcgcgagga
     6421 cagtcgggcc ggcgcaagcc ctatgaaatg aatatgcagg cggcgtgagc agcgagggga
     6481 tgccggcgct gcttggcgcc ggcgggggca gcatcagaac cggtaaccga aagcctgtgc
     6541 gaagcgcgtt tcgatttccg cgcgcgcggg cgcatggttt tgcccgccct gatgagcgat
     6601 cttgatcgag cccatcaggc tcgcaagacg ccccgtggtt tcccagtcca taccattcgt
     6661 cagcccatac atcagcccgg cacggaaggc gtcgccgcaa ccggtgggat cgaccacgcc
     6721 gtcggccggc acgcagggaa tgtcgatgcg gcgtcctgaa gcgtaaatct ccgagccgcg
     6781 ctcgccgcga gtcacgatca ggcccgttac gcgttctgca agcgcttcga gcgacaggcc
     6841 ggtgcgttcc atcagcagtt ccgcttcata gtcgttcacc gcgacatagg tcgccagttc
     6901 gaccaggtgc tcgagctctt tgccattgaa catcggcagt ccctggcccg gatcgaagat
     6961 gaacgggatg ccgagttccg cgcagtcgcg tgcgtgctgc agcatgccgt cacgtccatc
     7021 cggggcaatg atcgccagtt tcacggctcc cgcgtcggcc acccgattct ggtgcgagaa
     7081 gctcatcgca cccgggtgga aggcggtgat ctggttgccc ccttcatcgg tggtgatgta
     7141 ggcctgtgcg gtgtacgagg tctcgatcgt gcgcacgcaa cgggggctga ttcgcagctg
     7201 ctccaggcgt tcgagatacg gcccgccgtc gctgcctatg gttgccatga tcagcggatc
     7261 gccctccagc agtctgaggt tgtaggcgat gttgccggcg cagccgccga actcgcgccg
     7321 cacctcgggc accaggaatg ccacattgat cttgtgcagc tgatcggcga gcagtgcctc
     7381 ggagaagcgc ccgcggaaca gcatgatggt gtcgaaggcg agggaaccgc aaatcaggga
     7441 agtggtggtc atcgttctta aggtcagggg tagaagacgt agacgcgata gcccgacggc
     7501 tggatgcctt ccggttcgaa gaacaggcgc accggctgtt cgctgcgtgc ggcgaagccg
     7561 gtgcgcggat tgcttccgcc cggaaggtat tcggacgggg cgaatacgcg ccgcgcgagt
     7621 accttgccgg cgttgtcgtt gagcgtgagt tcaagataag gccaggattg cgcgactgtc
     7681 ccgtgattgc gcaacaaaac cgtcaatccc agcgtcgagg tgttcggtgc gagcgtctgc
     7741 agctcgctgg actcgatggc gaccgcttcg atctgcgccg gcaatccgac ctggcagccg
     7801 agataggcgc aagcctcatg cagccacggc gcggtctgcg gaaagcgcgc ggcaagctgg
     7861 tcgcggaaca catacgcgga ttgtgcgatc agggccacca gcagcagaac ggaaccggct
     7921 gacatcgtca cggtggcgag ccggcccatg cgctggcgcc gacggccctg gcgcacgaag
     7981 ctcggttcgt cttcgtcgcg aacggccgtg ccggcgccgc gcagggggcg gcgctgcagg
     8041 tcttcgattg cctggtcgag cgggtccggg ccttccgtcg cggcgggctc cggagcagtg
     8101 tggtgggatg
//
LOCUS       DATLGF010000348         3919 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_73646,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000348 DATLGF010000000
VERSION     DATLGF010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73646"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="VM406_10670"
     CDS             complement(<1..1180)
                     /locus_tag="VM406_10670"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HVL76468.1"
                     /translation="MNFRSVRIGARLGIGFGAILTWVVLVLIAGIVVSGNARRTHNDA
                     TQRANAKVAVANEMRSALLEGGLAMRNVGLQYSTPEMEKEEAKLKVQRKRFEEARATL
                     ASHGLSEAEQQIVAKITELDRQTEAPFKEAIELIKGYSNETAGKLISSRIDPLNQQAI
                     VEIDRLVGMQQARIDTLMAESEATGQMITWVLAGTGVLTVLLGGIVSLLTTRTITVPL
                     QEAVAVARRVASGRLGERIQVAGRDETSELLHALKDMDENLAKIVRQVRSGTDEISVA
                     SDEIAAGNADLSSRTESQASALEETASSMEELTSTVRHNAENARQANSLVQSASVSAT
                     KGGEVVADVVGTMDSIKQSSQKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     A"
     gene            complement(1340..2011)
                     /locus_tag="VM406_10675"
     CDS             complement(1340..2011)
                     /locus_tag="VM406_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL76469.1"
                     /translation="MPLLKILFILAMMAFAGTVRAAEPPDVLVKRVTEDVMQTARTDR
                     AIRSGDRSRIVEVVKDKILPHLDFERATRLAMGRHWREATPRQRQQMMDEFRTLMIYT
                     YAGALSQISDQRLDYRPLRLSPDDNEAEVRFRVLRPRRGEPVEVSYRMIRSPDGWKVY
                     DVNVLGVWITEVYRSSFSEEVTRNGVDGLISRLAERNRELRTRQVLTTPTPAEALPGS
                     GGSQG"
     gene            complement(2096..2317)
                     /locus_tag="VM406_10680"
     CDS             complement(2096..2317)
                     /locus_tag="VM406_10680"
                     /inference="COORDINATES: protein motif:HMM:NF023507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3562 domain-containing protein"
                     /protein_id="HVL76470.1"
                     /translation="MPILTDSDGAGAANDAGRHAAAVASLATELDRPIDEIEALYEDE
                     LERMRPQASIQDFLSILVSKRVRQTCRHR"
     gene            complement(2552..>3919)
                     /locus_tag="VM406_10685"
     CDS             complement(2552..>3919)
                     /locus_tag="VM406_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HVL76471.1"
                     /translation="LPNLPDLARPFGIVVTGVGGTGVVTIGALLGMAADLEGKGVSVL
                     DMTGLAQKGGAVLSHVQIAASLDALHATRVAAGEAALVIGCDAIVSASADVLTRTRHG
                     ITRAVINSAGTPTAEFVKNREWSYPASRVQSELKNGIGDGVEFIDANEAALTLLGDTI
                     YANPLLLGYAWQKGWLPVGRDALERAIELNGVSVEKNKLAFDWGRYLARHGAAALPRP
                     ASVAQPVVLQMPESLEKIVATRVAHLTAYQDAAYAAHYRDAVERVREKEKAVFGESRS
                     MPLTQAVAVNLAKLMAYKDEYEVARLYADPAFLDKLRSQFDGEPGRDYRLNFYLAPPL
                     LAKRNDNGELVKRRYGAWVLPAFRLLAPLRRLRGSWFDPFGRTAERRAERHLIDEYFA
                     LVDELCSSLSAENREAALELARLPDAIRGFGHVKEKAMEAAARRRAELLARYRAPCET
                     LRAAG"
BASE COUNT          599 a         1323 c         1295 g          702 t
ORIGIN      
        1 cggcaaagcc gcggccctgc tcaccggcac gcgccgcctc caccgccgca ttcagcgcaa
       61 ggatgttggt ctggaaggcg atgccgtcga tgacggagat gatgtcgacg atcttctgcg
      121 aactctgctt gatcgagtcc atcgtgccaa ccacgtcggc caccacttct ccacccttgg
      181 tcgccgacac cgacgcggac tggacaaggc tgttcgcctg gcgcgcattc tcggcgttgt
      241 ggcgcacggt ggaggtcagt tcttccatcg aggacgcggt ctcctccagc gcgctggcct
      301 gagactcggt gcggctggac aagtcggcgt tgccggcggc gatctcatcg gaagccacgc
      361 tgatttcgtc ggtgccgctg cgcacctggc gcacgatctt cgcgaggttt tcgtccatat
      421 ccttcagcgc gtgcagcagt tcgctggttt cgtcgcgtcc cgcgacctgg atgcgttccc
      481 cgagccgacc cgacgcgacc cggcgcgcaa ccgccaccgc ttcctggagc ggcaccgtga
      541 tcgtgcgggt ggtcagcagc gacacgatcc cgcccagcag cacggtcagc acgccggtgc
      601 ctgcgagcac ccaggtgatc atctggccgg tcgcttccga ttctgccatc agcgtatcga
      661 ttctcgcctg ctgcatgccg accaggcggt cgatttcgac gatggcctgc tggttgagcg
      721 gatcgatgcg ggaggaaatc agcttgccgg cggtctcgtt gctgtaaccc ttgatcagct
      781 caatcgcctc cttgaacggc gcctcggtct ggcgatcgag ctcggtgatc tttgcgacga
      841 tctgctgttc ggcttccgac aggccgtggc tggccagcgt cgcgcgcgcc tcctcaaagc
      901 gcttgcgctg caccttcagc ttcgcttctt ccttctccat ttccggcgtg ctgtactgca
      961 ggccgacgtt gcgcatcgcc agtccgccct caagcagcgc gctgcgcatt tcgttggcaa
     1021 ccgccacctt cgcattcgcc cgctgagtcg cgtcgttatg agtacgccgg gcgttgccgc
     1081 tgacgacgat gccggcgatg aggacgagca caacccaggt gaggatcgcg ccgaaaccga
     1141 tgcctaggcg agcaccgatc cggaccgagc ggaagttcat gacgggggtc tccttgctat
     1201 tgttttttcc aacgggggtc gaacgatctt agcgttgcga tttccattcg gcaactcaac
     1261 catcgttgaa ccccgcgttt tgcaaggatt ggcgtcgcgt gtgtcggatt tacgcgacgc
     1321 gggcggacgg cgcgagcatt cagccctgcg atccgccgct gcccggcaat gcctcggccg
     1381 gggtcggcgt cgtcaacacc tgtcgcgtcc tcagctcgcg gttcctttcc gccaacctgc
     1441 tgatcaggcc gtccacaccg ttccgggtca cttcctctga aaagctgctg cggtacacct
     1501 cggtgatcca cacgcccagc acattcacgt catacacctt ccagccgtct ggtgagcgga
     1561 tcatccggta gctgacctcc accggttcgc cgcggcgcgg ccgcagcacg cgaaagcgca
     1621 cctccgcctc gttgtcgtct ggactcaggc gtagcgggcg gtaatcgagc ctctggtcgc
     1681 tgatctgcga cagcgcgccg gcataggtgt agatcatcag cgtgcggaac tcatccatca
     1741 tctgctgacg ctgccgcggc gtggcctcgc gccagtggcg gcccatcgcc agccgggtcg
     1801 cgcgctcgaa atccaagtgc ggaaggatct tgtccttgac gacctcgacg atgcgcgagc
     1861 ggtcgcccga ccggatcgcg cggtcggtgc gcgcggtctg catcacgtct tcggtcacgc
     1921 gcttgaccag cacatcgggc ggctccgccg cacgcaccgt gccggcgaac gccatcatgg
     1981 cgaggatgaa gaggatcttg agcagtggca tggtgtccct catgcaggaa ggcaatcact
     2041 gtgcagcgag caagcatcag ccgtgcctgt gcgcggaggc gggcaggcgc ccccctcagc
     2101 gatggcggca ggtctgcctg acacgcttgg acaccaggat ggacaggaag tcctggatgc
     2161 tcgcctgggg ccgcatgcgc tcgagttcgt cttcgtagag cgcctcgatc tcgtcgatcg
     2221 ggcgatcgag ctcggtggcg aggctggcga cggccgcggc atggcggcct gcatcgttgg
     2281 cggcgccggc gccgtccgag tcggtaagaa tgggcatagc cgtgcagcgg acggcattgc
     2341 cggcggcagg caggcgccat ccgccgtacg cagggccggg gccgcgttca gtccagagac
     2401 ggtttcggac aagactttcg gaagaaagtt gtactgcccg gcgcaaatcg ggcggtgtaa
     2461 gcgtaagaat gcggttcacg gacattcacg gccgtgaacc gcaggtgcga atgcagccgg
     2521 tcgcgggccg gcgggatcgg gcggggtgcc gtcagccggc cgcgcgcagc gtctcgcacg
     2581 gcgcgcggta gcgggccaat agttcggcgc gccggcgcgc ggccgcctcc atcgccttct
     2641 ccttcacatg gccgaagccg cgtatcgcgt ccggcagccg cgccagttcc agcgccgctt
     2701 cacggttctc ggccgacagg ctgctgcaca gttcgtcgac cagcgcgaag tactcgtcga
     2761 tcaggtggcg ctcggcacgg cgttcagcgg tacggccgaa cgggtcgaac cagcttccac
     2821 gcagccggcg cagcggcgcg agcaggcgga acgccggcag cacccaggca ccgtagcggc
     2881 gcttgaccag ctcgccgttg tcgttgcgtt tggccagcag cggcggcgcg aggtagaaat
     2941 tgagccggta gtcgcggccc ggttcgccat cgaactggct gcgcagcttg tcgaggaagg
     3001 ccggatccgc gtacaggcgc gcgacctcgt actcgtcctt gtaggccatc agcttggcca
     3061 gattgacggc aaccgcctgc gtcagcggca tcgacctgct ctcgccgaac accgctttct
     3121 ccttttcgcg cacgcgctcg accgcgtcgc ggtagtgcgc ggcataggcg gcatcctggt
     3181 aagcggtcag gtgcgcgacg cgggtggcga cgatcttctc gagcgactcc ggcatctgca
     3241 gcacgacagg ctgggcgacc gacgccgggc gcggcagcgc ggcggcgcca tgccgggcca
     3301 gatagcggcc ccagtcgaac gccagcttgt tcttctcgac cgagacgccg ttcagctcga
     3361 tcgcgcgttc cagcgcgtcg cgccccactg gcagccagcc cttctgccat gcgtagccga
     3421 gcagcagcgg attggcatag atggtgtcgc cgagcagggt cagcgccgcc tcattcgcat
     3481 cgatgaattc gacgccgtcg ccgatgccgt tcttcagctc cgattgcacg cggctggccg
     3541 gatacgacca ttcgcggttc ttcacgaact cggcggtcgg cgtgccggcg ctgttgatca
     3601 cggcgcgcgt gatgccgtgg cgggtgcgcg tgagcacatc ggccgaggcg gagacgatcg
     3661 catcgcagcc gatcaccagc gcagcctccc cggcggcaac ccgggttgcg tgcagggcgt
     3721 cgaggctggc ggcgatctgc acgtggctca gcaccgcccc gcccttctgt gcgaggccgg
     3781 tcatgtcgag cactgatacg cccttgcctt ccaggtccgc ggccatgccc aggagcgcgc
     3841 cgatggtgac cacgccggtg ccgccgacgc cggtgacgac gatgccgaaa ggccgcgcca
     3901 ggtccggcag gttcggcag
//
LOCUS       DATLGF010000349         8082 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_93377,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000349 DATLGF010000000
VERSION     DATLGF010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 8082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8082
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93377"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VM406_10690"
     CDS             <1..605
                     /locus_tag="VM406_10690"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HVL76472.1"
                     /translation="TEVGGDLLTIEAMTMPGKGDTIRTGTLGDVMKESIEAARTVVRS
                     RAKRLGIKPELFTKSDIHIHVPEGATPKDGPSAGIAMTTAMVSVLTGIPVRADVAMTG
                     EITLRGEVLPIGGLKEKLLAAHRGGIKTALIPEQNVKDLADIPDNVKNKLEIIPVRWI
                     DKVLEVALERQPEPLRDVDTAVDTAVAAATGTHDPALVKH"
     gene            831..1103
                     /locus_tag="VM406_10695"
     CDS             831..1103
                     /locus_tag="VM406_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HVL76473.1"
                     /translation="MNKTELIDHIAKSADISKAASARAVEALIGAVRTTLKKDGSVTL
                     VGFGTFSVGKRAARTGRNPRTGDEIKIEAAKVPKFRPGKALKDAIN"
     gene            1195..1270
                     /locus_tag="VM406_10700"
     tRNA            1195..1270
                     /locus_tag="VM406_10700"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1228..1230,aa:Val,seq:tac)
     gene            1318..1394
                     /locus_tag="VM406_10705"
     tRNA            1318..1394
                     /locus_tag="VM406_10705"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1352..1354,aa:Asp,seq:gtc)
     gene            1486..3399
                     /locus_tag="VM406_10710"
     CDS             1486..3399
                     /locus_tag="VM406_10710"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HVL76474.1"
                     /translation="MFDFIRTHRRLMQFVLLLFIFPSFAFFGLEGYSRYRGDADAVAK
                     VGGQSITRQELDFAQREQMERLREMFGGQFDPRMLDTPEARRSILEGLIVERVLALEA
                     ADKRLAVSDQALQQTILEIPGLTGEDGRFDVERYRSLLAARGMTPTSFEARMRRDMVL
                     QQLGDAVQGSAIAPRTVTNRLSDLNDQEREVQELVFRTADFARQVNVTPEMLQRYYEA
                     NIRDFEVPARVDAEYIVLTMDALAERINVSEADIKAYYEQNAARFSEPEQRRARHILI
                     RTEPGASEADRAAARKKAEELLVQVRNAPGEFPRFAREHSQDPGSAAQGGDLDFFERG
                     MMVKPFEDAVFSLDKGEISDVVESDFGLHIIQVTDIKPGAVKSLDAVRGELVAEIRRQ
                     QATREFANATEQFSDLVFEQADSLQPAAEKLQLQIRKVEGLTRTPNPALGANAPVNHP
                     RFLTAIFSDDAIKNKRNTEAVEVAPGTLIAGRVLNYQPQTRRSLQEVEQVVRERVVRE
                     EAEAMARKAGEERLKALREGAQPQGFGAARNVSRVNAQGVAPQAVNAVMRADTTSLPA
                     YVGVELPEQGYAVYRINRVMQPEQPDTARRAAEQQQIANAIAQQEMHAYLESLKRKHK
                     VEVLSVPARAEEQ"
     gene            complement(3562..4155)
                     /locus_tag="VM406_10715"
     CDS             complement(3562..4155)
                     /locus_tag="VM406_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HVL76475.1"
                     /translation="MLATLLFAASAYSASKTILVLGDSLSAEYGITRGAGWVTLLEQR
                     LKKESIGAQVVNASISGETTSGGRARLSALLTQHKPDIVVIALGGNDGLRGLPLSAAE
                     SNLREMIRASQQANARVLLVGMRIPPNYGREYTERFFDMYGKLARETKVPLVPFFLEG
                     VAERQELFQSDRIHPTAEAQPVMLQNVWPKLKPMLLR"
     gene            4214..4900
                     /locus_tag="VM406_10720"
     CDS             4214..4900
                     /locus_tag="VM406_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL76476.1"
                     /translation="MPELSSSIPAIEVARLGKRVADATGELTILRDIDFTVASGETLA
                     IVGASGSGKSTLLGLLAGLDTPTEGTVKLFGVDIFALDEDGRAGLRQAKLGFVFQSFQ
                     LLSHLTALENVMLPLELRREDGAREKAEAMLERVGLASRLRHYPKYLSGGEQQRVALA
                     RAFVTAPPLLFADEPTGSLDAATGEAVIRLMFEMNEERGSTLVLVTHDPSIAARCRRK
                     VTITAGRLSE"
     gene            complement(4902..6437)
                     /locus_tag="VM406_10725"
     CDS             complement(4902..6437)
                     /locus_tag="VM406_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HVL76477.1"
                     /translation="MRTRSTPPPRPVPLYFTEQVRTLERAALDTTPEGTLMARAGKAA
                     ADLALRLLGSAAGARVLVLAGPGNNGGDALVAARHLTGMQAQVALLPYYVDTAPCPDD
                     ARRARNEYQAAHGGWVAPVPESCDGNWDLVIDGLFGIGLRRGFDTTTAAIVRAVNRLR
                     CPVLALDVPSGLDADTGQPVDMDAGAVRATHTITFIADKPGLYTGLGRDHAGEVTLAD
                     LNLDRAIYPPSDLHLATIGAFQPALRQRRHGSHKGSHGDVVVAGGAHGMTGGAILAAR
                     AALFCGAGRVYAALLDRTTSYDAPHPELMLRSADGIEFARAVIVAGQGMGRDEAARRL
                     LERALDAGRPTVLDADALNLVAADTDLQRKLASRGQDFILTPHPLEAARLLGQNAEQI
                     QRARIDTARMLARRFNCIAVLKGSGTVIAAPDGQAMINPTGNAGLATAGSGDVLAGIC
                     GALLAQGFSPWHAALAGVWTHGAAADSLVEEGIGPIGLTASELIPRVRRLLNQLTGQY
                     AES"
     gene            complement(6485..7603)
                     /locus_tag="VM406_10730"
     CDS             complement(6485..7603)
                     /locus_tag="VM406_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVL76478.1"
                     /translation="MDRLDCVVVGAGVVGLAVARALALAGREVFVIEKHPAIGMETSS
                     RNSEVIHAGLYYPTGSLKARLCVQGRDALYAYCDERGLPYRRCGKLIVATSDAQLARL
                     GAIHAQAHANGCAEVVHISAAEAQAMEPALSCVAALHSPNTGIIDSHALMLALQGDAE
                     NAGALFAFKTTVLGGVAAQNGIVLRTVDDNGDEMEIEAACVVNCAGLWSTHLARGLHG
                     LDATDLPIAHFAKGNYYSLAGRAPFSQLIYPVPEPGGLGVHLTLDLGGQARFGPDVEW
                     LDQADREPDYAVDPRRAERFYREIRGYWPQLPDGALQPAYSGVRPKISGPGADAADFL
                     FTNHGNRHYLGLYGIESPGLTASLAIAEHAQRLVARIA"
     gene            complement(7596..>8082)
                     /locus_tag="VM406_10735"
     CDS             complement(7596..>8082)
                     /locus_tag="VM406_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVL76479.1"
                     /translation="SADLLRPLGIDVPMYPVRGYSLTAMIRDYDKVPQATVVDEAYKV
                     AITRLGGRVRVAGTAELGAATPALRERALRTLRKVAGDWFPQAAQYAEANTWQGVHPM
                     LPDGTPLLGATPVRDVYVNIGHGTEGWSMAVGSGKIVADIVSGQTPEVDMEGLTLQRY
                     G"
BASE COUNT         1393 a         2596 c         2716 g         1377 t
ORIGIN      
        1 ggaccgaagt gggcggcgac ctgctcacga tcgaagcgat gacgatgccg ggcaagggcg
       61 acacgatccg caccggcacg ctcggcgacg tgatgaagga gtcgatcgag gcggcacgta
      121 ccgtggtacg cagccgcgcc aagcggctgg gtatcaagcc cgagttgttt accaagagcg
      181 acatccacat ccacgtgccg gagggcgcga cgccgaagga cgggccgtcc gccggtatcg
      241 cgatgaccac ggcgatggtg tcggtcctga ccggcatccc ggtgcgcgcc gacgtggcga
      301 tgaccggcga gatcaccctg cgcggcgagg tgctgccgat cggcggcctg aaggagaagc
      361 tgctggcggc ccaccggggt ggcatcaaga ccgcgttgat tccggagcag aacgtcaagg
      421 atctggccga cattccggac aacgtgaaga acaagctcga gatcatcccg gtacgctgga
      481 tcgacaaggt gctcgaagtg gcgctggaac ggcagcccga gccgctgcgc gatgtcgaca
      541 ccgccgtgga tacggccgtc gcggcggcga cggggactca cgatccggcc ctcgtcaagc
      601 actgagcgaa caacattcgc ccgtcctgaa aaagcgctcc ggtggggcgc ttttttgttg
      661 cacccctcgt attcatgcgg cttcgcaggg gatgccagct tgacactggg cagggtggct
      721 tgtctaatac gggctgcaga aaatttcttg actgcatgtc gccgcaacca agcaggcaaa
      781 attccgataa atcgggatgc gcgacaccat tatttcttga gggggcatac gtgaacaaaa
      841 ccgaattgat cgatcacatc gccaagtccg ccgacatttc caaggcggcc tcggcgcgtg
      901 cggtcgaagc gctgatcggc gccgtcagga ccacgctgaa gaaggacggc agcgtcacgc
      961 tggttggctt tggcactttt tccgtcggca agcgggccgc gcgcaccggc cgcaatcctc
     1021 gcaccggcga cgagatcaag atcgaagctg ccaaggtgcc caagttccgg ccgggcaagg
     1081 cgctgaagga cgcaatcaac taattgctgc gttcggaaaa aagctgttgt agaatcttgc
     1141 ttttcccgag gtggtgacaa acgtcgggaa gtttggctga cttagtgtgg cacggggtgc
     1201 ttagctcagt tggtagagcg gcgcccttac aaggcgtagg tcgggagttc gagcctctca
     1261 gcacccacca ggcagccaga cggcagtacg aggtccaagc gaggacttca gatttcagga
     1321 gtggtagttc agtcggttag aataccggcc tgtcacgccg ggggtcgcgg gttcgagtcc
     1381 cgtccactcc gccaaaacaa gaaaaggcga acgcgagttc gccttttttc ttatgcgccg
     1441 cgtatcggct cctgtgtcga tgccgcgcca gtaactggat tgaacatgtt cgatttcatt
     1501 cgcacgcatc gccggctgat gcagtttgtg ctgcttctgt tcatctttcc ctcctttgcg
     1561 tttttcggcc tcgaaggcta cagccgttac cggggcgatg ccgatgcggt ggcgaaggtc
     1621 ggcggtcagt cgataacccg gcaggagctc gatttcgccc agcgcgaaca gatggagcgg
     1681 ctgcgagaga tgtttggcgg ccagttcgat ccgcgcatgc ttgatacccc tgaagcgcgc
     1741 cgcagcatcc tggaaggcct catcgttgag cgcgtgctcg cgctggaggc ggccgacaag
     1801 cgcctcgcgg tgagcgatca ggccttgcag cagaccatcc tcgagattcc gggcctgacc
     1861 ggcgaagacg gtcgcttcga tgtggagcgc tatcgcagcc tgctcgccgc gcgcggcatg
     1921 acgccgacgt ccttcgaggc gcggatgcgt cgcgacatgg tgttgcagca gcttggcgac
     1981 gcggtccagg gcagcgccat cgctccgcgc accgtgacga accgcctttc ggacctgaac
     2041 gaccaggagc gcgaggtgca ggagctggtg ttccgcaccg cggacttcgc caggcaggtg
     2101 aacgtcacgc cggaaatgct gcagcgttac tacgaagcga acatccgcga cttcgaagtg
     2161 ccggcgcgtg tggatgcgga gtacatcgtg ctgacgatgg acgcgctggc cgagcgtatc
     2221 aacgtcagcg aggcggacat caaggcttac tacgagcaga atgcagcgcg cttttccgag
     2281 ccggagcagc gccgcgcacg ccatatcctg atccggaccg aaccgggcgc gtctgaagcc
     2341 gaccgcgccg ccgcgcgcaa gaaggcggaa gagctgcttg tccaggtgcg caacgcgccg
     2401 ggcgaattcc cgcgcttcgc gcgcgagcac tcgcaggatc cgggttcggc cgcgcaaggc
     2461 ggcgatctcg atttcttcga gcgcggcatg atggtcaagc cgttcgaaga cgcggtgttt
     2521 tctctcgaca agggcgagat cagcgatgtg gtggagtccg atttcggctt gcacatcatc
     2581 caggtcaccg acatcaagcc gggcgcggtc aagagcctcg atgcggtacg cggcgagctg
     2641 gttgcagaga tccgtcgcca gcaggcgacc cgcgagttcg ccaatgcgac tgaacagttc
     2701 tccgatctgg tgttcgagca agccgacagc ctgcagccgg ccgccgaaaa gctgcagttg
     2761 cagatccgca aggtcgaggg gctcacccgc acgccgaatc ctgcgctagg ggcgaatgca
     2821 ccggtcaacc atccgcgttt cctgaccgcg atcttctcgg acgacgcgat caagaacaag
     2881 cgcaacaccg aagcggtcga ggttgcgccc gggacgctga ttgccggccg cgtgctcaac
     2941 tatcaaccgc agacccggcg ttcgctgcag gaagtcgagc aggtcgtgcg tgagcgcgtg
     3001 gtgcgcgagg aagccgaggc gatggcgcgc aaggcgggcg aggagcgcct gaaggctttg
     3061 cgcgaaggcg cacagccgca gggcttcggc gccgcccgca atgtctcgcg cgtgaatgcg
     3121 caaggggtcg cgccgcaggc cgtgaacgct gtcatgcgcg ccgacacgac gagcctgccg
     3181 gcctatgtcg gcgtggagtt gccggagcag ggctacgcgg tgtaccggat caatcgcgtg
     3241 atgcaacccg agcagcccga caccgcccgc cgcgctgccg agcagcagca gatcgcgaac
     3301 gcgatcgcgc agcaggaaat gcacgcgtac ctggagtcgc tcaagcgcaa gcacaaggtt
     3361 gaagtgctgt ccgtgccggc acgggcggaa gagcagtaac gcgcgctctc gtcatcgttg
     3421 aaaacgccct ccggtgatgg agggcgtttt tatttggaga ttcgattcca cttgctatgt
     3481 ggttccccta ggcgggaaca agttcaggcg ccggcgcccg ccgcgtcggc acgccaggct
     3541 gcagcgcccc agcctgccag gtcagcgcag cagcattggc ttcaatttcg gccacacatt
     3601 ctgcagcatc actggttgcg cttcggcggt cggatgaatg cggtcgctct ggaacagttc
     3661 ctggcgttcg gcgacgcctt caaggaagaa tggaaccagc ggcaccttgg tttcccttgc
     3721 gagcttcccg tacatgtcga agaagcgctc ggtgtattcg cgtccgtaat tcggcggaat
     3781 gcgcatgccg accagcagca cgcgtgcgtt cgcctgctgg gacgcgcgga tcatctcgcg
     3841 caggttgctt tctgccgcgc tcagtgggag cccgcgcagg ccatcgttgc ctcctaatgc
     3901 gatcacgacg atgtccggct tgtgctgtgt cagcagcgcc gacaggcgag cacgcccgcc
     3961 gctcgtcgtc tcgccgctga tgctggcgtt gacgacctgg gcgccgatgc tttccttctt
     4021 caggcgctgc tcgagcagcg ttacccaacc ggcgccgcgc gtgatcccgt actcggccga
     4081 cagactgtcg ccgagcacca ggatggtttt tgatgcagaa taggcgctcg ctgcgaacag
     4141 cagcgtggcg agcatcacca ggctgcgcag cagcacccac acaccattcc ctgaccttcc
     4201 catcactttc tgcatgccgg agctatcctc ttccattcct gccattgaag ttgcgcgcct
     4261 cgggaagcgt gtcgccgatg ccacgggcga actcacgatc ctgcgcgata tcgattttac
     4321 tgtcgcatcc ggagagacgc tggcgatcgt cggcgcttcc ggctcgggga agtcgacgct
     4381 gctcggcctg ctcgcggggc tggacacacc gaccgaaggc accgtaaagc tcttcggcgt
     4441 ggacatcttc gcattggacg aggacgggcg cgcagggttg cgtcaagcga agctgggctt
     4501 tgtattccag tccttccagc tgctttcaca cctgaccgcg ctggagaacg tgatgctgcc
     4561 gcttgaactg cgccgtgagg acggcgcgcg cgagaaggcc gaagccatgc tcgagcgcgt
     4621 gggcctggcg agccgcctgc gccactaccc gaagtacctg tccggcgggg aacagcagcg
     4681 ggtcgcgctg gcgcgcgcgt tcgtgactgc gccgccgctg ctgtttgccg acgaaccgac
     4741 gggtagcctg gacgccgcca ccggcgaggc ggtgatccgt ctgatgttcg aaatgaacga
     4801 agagcgcggc tcgacgctgg tactggtcac gcacgatcct tcgattgccg cgcgttgccg
     4861 ccgcaaggtc acgatcaccg ccgggcggct gtccgagtga gtcaactctc ggcgtactgc
     4921 ccggtcagct gattgagcag gcggcgcacg cgcgggatca gttcgcttgc cgtcaggccg
     4981 atcggcccga tgccttcctc gaccagcgaa tcggccgccg caccgtgggt ccagacgccg
     5041 gccagcgcgg catgccatgg cgagaacccc tgggcgagca gggcgccgca gatgccggcg
     5101 aggacgtcgc cgcttccggc ggtggcgagt ccggcattgc cggtggggtt gatcatcgcc
     5161 tgcccatccg gcgcggcgat caccgtgccc gaacctttca gtaccgcaat gcagttgaag
     5221 cgccgggcga gcatgcgcgc ggtgtcgatg cgcgcgcgct ggatctgttc ggcgttctgt
     5281 cccagcagac gtgcggcttc gagcggatgc ggcgtgagga tgaaatcctg gccgcgcgag
     5341 gccagcttgc gctgcagatc cgtgtcggcg gcgacgaggt tgagtgcatc cgcatccagc
     5401 accgtcggcc gcccggcatc gagcgcgcgt tcgagcaacc tgcgtgcggc ttcgtcacgc
     5461 cccatgcctt gtccggccac gatcaccgcg cgtgcgaact cgatgccgtc ggccgaacgc
     5521 agcatcagct ccggatgtgg cgcatcgtaa gacgtggtcc ggtcgagcag ggcagcgtag
     5581 acccgcccgg cgccgcagaa aagggccgcg cgcgccgcca gtatcgcgcc gcccgtcatg
     5641 ccgtgtgcac ctcccgccac caccacgtcg ccatggctgc ccttgtgcga gccgtggcgc
     5701 cgctggcgca gagccggctg gaatgcgccg atggtggcca ggtgcagatc ggagggcggg
     5761 tagatggcgc ggtccagatt gagatccgcg agcgtcacct cgccggcgtg gtcgcggccc
     5821 aacccggtgt acagaccggg tttgtcggcg atgaacgtga ttgtatgggt ggcgcgcacg
     5881 gcgcccgcgt ccatgtcgac cggttgcccg gtatccgcgt ccaggccgct gggcacgtcg
     5941 agggcgagca ccggacagcg cagccggttg actgcccgca cgattgccgc cgtggtggtg
     6001 tcgaagccgc ggcgcaggcc gattccgaac agcccatcga tgaccagatc ccagttcccg
     6061 tcacaggact cgggcaccgg cgcgacccag ccgccatgcg cggcctggta ttcattgcgc
     6121 gcgcgccggg catcatccgg gcatggcgcg gtgtcgacgt aatagggaag cagggcgacc
     6181 tgagcctgca tgccggtaag gtgccgcgcc gcaacgaggg catcgccgcc attgtttccg
     6241 ggaccggcca agaccagcac gcgcgcaccg gcggcgctgc cgagcaggcg caacgcgagg
     6301 tcggctgccg ccttgccggc gcgcgccatc agggtgccct caggcgtcgt atccagtgcc
     6361 gcgcgctcga gcgtgcggac ctgttccgtg aaatacagag ggacggggcg gggcgggggt
     6421 gtcgaacgtg tgcgcacgat gcttgcctcc tggatccagc ggacgaacga aggtgctcgc
     6481 gattctatgc gatccgcgcg accaggcgct gcgcatgctc agcgattgcg agcgatgccg
     6541 tcaagccagg cgattcgatg ccgtacagac cgagataatg ccgattgcca tggtttgtga
     6601 acaggaaatc ggcggcatcc gcgccgggcc cgctgatctt cggccgtaca cccgaatagg
     6661 cgggctgcag cgcgccatcg ggtagttgcg gccagtagcc gcggatctcg cgatagaagc
     6721 gttccgcacg gcgcggatcg accgcgtagt ccggttcgcg gtcggcctgg tccagccact
     6781 ccacgtcggg cccgaagcgg gcctgcccgc cgaggtcgag cgtcaggtgt acgccgaggc
     6841 cgcccggttc gggaaccgga tagatgagtt gcgagaacgg cgcgcgtccg gcgagtgaat
     6901 agtagtttcc tttggcgaag tgcgcgatcg gcagatcggt cgcgtcaagg ccgtgcagcc
     6961 cgcgcgcaag atgcgtgctc cagaggcccg cgcagttgac gacacaggcg gcttcgattt
     7021 ccatctcgtc gccgttgtca tcgacggtgc gcagcacgat gccgttctgc gctgccacgc
     7081 cgccgagcac ggtggtcttg aacgcgaaca gtgcgccggc gttctccgca tcgccttgca
     7141 aggccagcat cagcgcatgg ctgtcgatga tgccggtgtt cggtgagtgc agggctgcga
     7201 cgcacgacag ggccggttcc atggcctggg cttcggcagc gctgatgtgc accacttccg
     7261 cgcagccgtt cgcatgcgcc tgcgcgtgga tcgcacccag gcgcgcgagc tgcgcgtcgg
     7321 acgtggcgac gatcagcttg ccgcagcgcc ggtagggcag gccgcgttcg tcgcagtacg
     7381 cgtacagcgc atcgcgtccc tgcacgcaca gtcgggcctt gaggctgcct gtcgggtaat
     7441 acaggccggc gtgaatcacc tcgctgttgc gcgagctggt ctccatgccg atcgccgggt
     7501 gcttctcgat tacgaacacc tcgcgcccgg ccagcgccag cgcccgcgcg acggcaaggc
     7561 ccacgacccc ggcgccgacc accacgcaat ccagtctatc catatcgctg cagagtcagt
     7621 ccttccatgt ccacctcggg cgtctggccg gagacgatgt ccgccacgat cttgcccgag
     7681 cccactgcca tcgaccagcc ttcggtgccg tggccgatgt tgacatacac gtcgcgcacc
     7741 ggcgtggcgc ccaacagcgg agtcccgtcc ggcagcatcg ggtgcacgcc ttgccaggta
     7801 ttcgcctccg cgtattgtgc cgcctgcggg aaccagtcgc ccgcgacctt gcgcagcgtg
     7861 cgcagcgcac gctcccgcaa tgccggggtt gcggcgccga gctcggcggt gccggcgacg
     7921 cgcacgcgtc cgcccagtcg tgtgattgcc accttgtacg cctcatcgac gacggtcgct
     7981 tgcggcacct tgtcgtagtc gcgaatcatt gcggtgagcg aataaccgcg caccgggtac
     8041 atcggcacat cgatgccgag cggcctgagc agatcggcgc tg
//
LOCUS       DATLGF010000350         6784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_30743,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000350 DATLGF010000000
VERSION     DATLGF010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6784
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30743"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VM406_10740"
     CDS             <1..615
                     /locus_tag="VM406_10740"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVL76480.1"
                     /translation="AAAPSLALGFLPRVMQRFMQACPDVAISLHTNTSATVEHWTRLQ
                     YCDLGLAVALSEGCDARVELLGQANGVCILPRGHRLAALPEITPAALRGETLILPAHG
                     DGARERIEAIFRDAGVELAPRLEAQYAAACCAMVGLGMGVAIVNPLVVRDYCHMGIEV
                     RPFAPALPFPAWMLAPANRPTGLLAERFAATVRAVLQEEMETFA"
     gene            complement(612..1535)
                     /locus_tag="VM406_10745"
     CDS             complement(612..1535)
                     /locus_tag="VM406_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVL76481.1"
                     /translation="MTQQLRQLPPLDAIKGFVAVGRRMSITAAADDLCLTQSAVSRQI
                     QSLEEYFRTPLLIRKHRAIELTDAGRQLYELAAPWFEELAEFTRIVRQERRLQPVTVT
                     ASIGVASLWILPRLGGFQAQQPNVDVRLAASDKVLDMQQEGIDLAIRYCSAASAPPDA
                     VKLFDEEVVLVAAPALAEKAFAGPEALLDQVLLEFDDRTRPWLRWSDWLRAMGLGNRQ
                     PKSTLHFNHYDQVIRAALDGSGVALGRTALIAPMLADGRLVAGPGKGLQASGYGYWLV
                     QATQQPRPEVALFRDWLLEQARQGGLHDEAA"
     gene            complement(1637..2137)
                     /locus_tag="VM406_10750"
     CDS             complement(1637..2137)
                     /locus_tag="VM406_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HVL76482.1"
                     /translation="MSVTTLKSLFAYKAWANTELFALLDTLPSEHSDEAHTCIRTLNH
                     IYVVDRLFRARLAGESIPFNATNTNETPTLADLRAEVGSTDAWYVQYVSSLADPALSE
                     VLDFTFADGDRGRMSREEVLLHVITHGAYHRGNVGQVLKSISIAPPRDLYTKFLHQSE
                     PTRREA"
     gene            2425..3105
                     /locus_tag="VM406_10755"
     CDS             2425..3105
                     /locus_tag="VM406_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76483.1"
                     /translation="MQFGLHPSPVLNALFQARPYQGCTPEDAWVVMLGSDANYSPEIS
                     EHAFFGTIVRYHEDGPGFVKRHKVHHPFLLNEYPFDRRTGGVLFHRNIATLLTLEVAA
                     RLSFIELLDVPTIGSRSSTPEAQAAYRRLFSRRHLQRLDDFVHRAQGKLVLLPAGVLQ
                     EMLHLHSLYGLFSWAEPLQTRDSLTLPGGARVRRVYHPSSSHFYSSAATVKKEISDWV
                     AQRRSDSA"
     gene            3136..3879
                     /locus_tag="VM406_10760"
     CDS             3136..3879
                     /locus_tag="VM406_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76484.1"
                     /translation="MKRIFVPTMTGADWQRLLGKPDLHWKKGRSAMSAAACWEQCHPR
                     LPSEVTSVLDSSDDPALSQLELLAAFPEWGVELPGGATVSQTDILAIARNRRGLVVLG
                     VEAKVDEPFGPTLGEKKHAASAGQLSRIAFLEKELGRTAVFDDRIRYQLLHRTVSALL
                     TARAFHASVAVMLIHSFSPISRWREDFESFCQALDCRSNSADLREVPGIQGPRLLLGW
                     CAGQEKFLEADLPSAFARIPPTGAPSVSS"
     gene            complement(3954..4076)
                     /locus_tag="VM406_10765"
     CDS             complement(3954..4076)
                     /locus_tag="VM406_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76485.1"
                     /translation="MKTGTGALLAAIGLLIWLAAAASLLGPAKLQSIVGLFALC"
     gene            complement(4087..5289)
                     /locus_tag="VM406_10770"
     CDS             complement(4087..5289)
                     /locus_tag="VM406_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL76486.1"
                     /translation="MNTLRINPLAAILCGGIIMGLALGIRHGQGLFLLPMTAERGWSR
                     EEFAFAIALQNLLWGLAQPFTGMIADRFGSLRVIFAGCLLYGLGLFWMAHAATPLALT
                     LSGGLAIGMALSATAFGTVYGALARMVPPERRGWALGLAGAVGGLGQFVAVPVAHGFI
                     DGFGWMAALVILALAIAALAPLAFTLTDNPSATVAPGARQGMGSAIGEAFRHRGFLLL
                     NLGFFACGFQLAFIGGHLPAYLADHGMNARAAVIALSLIALANVAGTFLFGRWGDVYR
                     RKHLLAWVYLARSALMVAFLALPLSELSVYVFAFGMGFLWLGTVPLTSGLISQVFGVQ
                     YISTLFGFVFVGHQIGGFLGMWLGGRVYDLTGSYDLIWIGAVGIGVVAAALHWPIDDR
                     QVARLRMA"
     gene            5497..6357
                     /locus_tag="VM406_10775"
     CDS             5497..6357
                     /locus_tag="VM406_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HVL76487.1"
                     /translation="MPREYTFRIVNVFAEEKFGGNPLCVFEDGRGLSDAEMQALARQF
                     NLSETTFILPSECADARVRIFTPGYELPFAGHPTLGSAHVVRAAGDGGDAVSLEFKAG
                     VVPVRAKGDLWSFTAPSLAAGAVPVYPPKEPRALIAALVGLNESDLAADPAWVHAGVD
                     QLLIPVRDAGTVRRAAPDSSLLDRWPQSSLGRKTGYVFAFTGDAVLARYFFTTQGGGV
                     SEDPGTGSACANLGAWMRHGGRQPPFSVRVSQGEAVQRPCVLYLDVNASGQISVGGTV
                     RELGRGTITL"
     gene            6459..>6784
                     /locus_tag="VM406_10780"
     CDS             6459..>6784
                     /locus_tag="VM406_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HVL76488.1"
                     /translation="MQSLWVLAATFLFSIMGVCVKLAADQHGLGEILFYRGAIGVFVI
                     LLICRLRFAPLRTPHLGLHFKRGAVGLISMSLWFYTIAVLPLPTAMTLAYTSPLWVVA
                     FLLVAAV"
BASE COUNT         1227 a         2242 c         2138 g         1177 t
ORIGIN      
        1 gccgcggcgc cctcgctggc gctcggcttc ctgccgcgcg tgatgcagcg cttcatgcag
       61 gcctgccctg acgtggcgat ctcgttgcac accaacacgt ccgccaccgt cgaacactgg
      121 acgcggctgc agtactgcga cctgggactc gcggttgcgc tcagcgaggg ctgcgacgca
      181 cgggtggaac tgctcggcca ggcgaacggc gtatgcatcc tgccgcgcgg ccatcggttg
      241 gcagcgctgc cggaaatcac gccggccgcg ctgcgcggtg agacgctgat cctgccggca
      301 cacggcgacg gcgcgcgcga gcggatcgaa gcgatcttcc gtgacgccgg cgtggagctt
      361 gcgcctcggc tggaagcaca gtacgcggcc gcctgctgcg cgatggtcgg actcggaatg
      421 ggcgtggcga tcgtcaatcc gctggtcgtg cgcgactact gccacatggg gatcgaggtg
      481 cgcccgttcg cgccggcgct gccgtttccg gcctggatgc tcgctcccgc gaaccggccg
      541 accgggttgc tcgcggaacg gttcgctgcg accgtgcgtg cggtgctgca ggaagagatg
      601 gaaacattcg cttaggccgc ctcgtcgtgc aggccgccct gccgcgcctg ctccagcagc
      661 cagtcgcgaa acagcgcaac ctccgggcgc ggctgctgcg tggcctgcac cagccaatag
      721 ccatatccgc tcgcctgcag ccccttgccg ggcccggcaa ccaaccttcc gtcggccagc
      781 atcggcgcga tcagcgcggt gcgaccgagc gccacgccgc tgccatccag tgccgcgcgg
      841 atcacctggt cgtagtggtt gaagtgcagc gtggacttcg gctggcggtt gcccagcccc
      901 atggcccgca gccagtccga ccagcgaagc cacggccggg tgcggtcgtc gaattcgagc
      961 agaacctggt cgagcaacgc ctccggaccg gcgaacgcct tctccgcgag cgcgggcgcg
     1021 gccaccagca cgacctcctc gtcgaacagc ttgaccgcat ccggcggcgc gcttgccgca
     1081 ctgcagtagc ggatcgccag gtctatgccc tcctgctgca tgtcgagcac cttgtcgctg
     1141 gcggccaggc gtacgtccac gttcggctgc tgcgcctgga agcctcccag ccgcggcagg
     1201 atccagaggc tggccacacc tatgctggcg gtgacggtaa ccggctgcag gcgccgctcc
     1261 tggcgcacga tgcgggtgaa ctccgccagt tcctcgaacc acggtgcggc aagctcatag
     1321 agctggcggc cggcgtctgt caattcgatc gcgcggtgct ttcggatcag gagcggcgta
     1381 cggaagtact cttccagcga ctggatctgg cggctgacgg cggactgggt caaacacaga
     1441 tcgtcggcgg cggcggtgat gctcatgcgc cgcccgacgg cgacgaagcc cttgatcgcg
     1501 tcgagcggag gaagctgtcg aagctgttgg gtgatcatgc ggtgggcgag cgaaaagagt
     1561 gaaggtttca tcttactctc gggggagcag ttcgcccgga tgcggcacac gtcgcccgat
     1621 agccatttag gaagagttag gcttctcgtc gagttggctc gctctggtgg agaaactttg
     1681 tatagagatc acgaggcgga gcaatcgaga tggacttgag aacctggccg acattcccgc
     1741 ggtgatacgc accgtgggta attacgtgaa gcaggacttc ttcccgcgac atacgccccc
     1801 gatcgccgtc agcaaacgtg aaatccagaa cctcagaaag tgcgggatcc gcaagagacg
     1861 agacgtattg gacgtaccaa gcatctgtgg acccgacttc ggcacgaagg tcagcgagcg
     1921 ttggtgtttc attggtgtta gttgcgttga acggaatcga ctctccggct aggcgggcac
     1981 gaaagagccg gtctacgacg tagatgtgat tgagggtacg gatgcacgtg tgggcctcgt
     2041 cggaatgctc cgatggcaag gtgtccaaca gcgcgaacaa ctcggtattc gcccaggcct
     2101 tgtaggcgaa cagggacttc agagtagtga ctgacatagc gactccacga tccgatcttc
     2161 attggaaagc ctggcaaccc gcctgcacag ggccctctcg gccgcatcct cggtttccgc
     2221 gactagataa caagacagac aaaccacgga tctcgtccaa gtccgtttca aaggatgaac
     2281 aaggttcaag tcaagatatc gtaccccctt aagcgcaagt ccgggccgct gcaaaacgtg
     2341 gacataactc aaatctttcg atgccgacgc gagcatcatg catgaacagt tcctgcagac
     2401 gagaaatcgc acaagggcat atgcatgcag ttcggcctcc acccgtctcc tgtcctcaat
     2461 gccttgttcc aggcccggcc gtatcaaggg tgcacgcccg aagacgcatg ggtggtcatg
     2521 ctcggatcgg acgccaacta ctcccctgag atatctgaac atgccttctt cggcacgatc
     2581 gtccgctacc acgaagacgg gcctggtttc gtaaaacgcc ataaggtcca ccatcctttc
     2641 ctgctgaacg aatatccgtt cgatcgacgg accgggggcg tcctctttca ccggaatatc
     2701 gccaccctgc tgacgctcga agtcgccgcg cgactatcgt ttatcgagct tctcgacgtg
     2761 ccgaccatcg gcagcagatc gtctacgccg gaagcgcagg cagcataccg ccgcctcttc
     2821 tcaaggcggc atctgcagcg gctcgacgac ttcgttcata gggcgcaagg aaagctggtt
     2881 ctcttgccgg cgggcgtgct gcaggaaatg ctgcacctgc actccctcta cggcctgttc
     2941 tcctgggcag agcccctgca gacccgggat tccttgactc ttccgggtgg cgcgcgggtt
     3001 cgcagggtgt atcacccatc gtcaagccac ttctacagca gcgcggcaac ggtgaagaag
     3061 gagatcagcg actgggtcgc gcagcgccgc tccgattccg catgaacaac cactaaccga
     3121 gcgtcgacaa atactgtgaa gcgcatcttc gtaccgacaa tgacaggcgc cgactggcag
     3181 cgcctgctcg gcaaacccga tctgcactgg aaaaaggggc gctctgcgat gtctgccgcg
     3241 gcgtgctggg agcaatgcca cccgcgtctc ccatccgaag tcacaagcgt gctcgactca
     3301 tccgacgatc ccgcgctctc ccagcttgaa ctgttggcgg cgttcccgga atggggtgta
     3361 gagcttcccg gcggagcaac cgtatcgcaa accgatattc tcgcgatcgc gcgaaaccga
     3421 cgtggcctgg tggttctcgg cgttgaggca aaggtcgatg agccgtttgg cccgaccctt
     3481 ggagaaaaga agcacgctgc gagtgcagga cagctcagcc gcatcgcctt tcttgagaaa
     3541 gaactgggcc ggacggctgt tttcgacgac cgcattcgct atcagctctt gcacagaacg
     3601 gtgtctgccc tgcttaccgc gcgggcgttt catgcttcgg tcgcagtgat gctgattcat
     3661 tcgttcagcc cgatctctcg ctggcgcgag gatttcgaaa gtttctgcca ggcgctcgac
     3721 tgccggagca attcagcgga tctgcgagag gtcccaggca ttcaaggccc gcgcttgctt
     3781 cttggctggt gtgccgggca agagaaattt ctcgaagcgg accttcccag tgcattcgca
     3841 cgcattcccc cgaccggcgc cccatcggtt tccagctagc gccgtccttc ggtcgaaccc
     3901 ggctgacgga actgttcgag cctgacccgc aggtcacacc ccgccccccg ctatcagcaa
     3961 agcgcaaaca gcccgacgat cgactgcagc ttcgccgggc caagcaggct cgcagccgcc
     4021 gccagccaga tcagcagccc gatcgcagca agcagcgcgc cggtaccggt cttcatcgcc
     4081 tgcccctcac gccatgcgca gccgcgccac ctgccggtca tcgatcggcc agtgcagcgc
     4141 cgcggcaacg accccgatgc ctaccgcgcc gatccagatc aggtcatacg agccggtcag
     4201 gtcgtacacc cggccgccca gccacatgcc gagaaagccg ccgatctggt ggccgacgaa
     4261 cacgaagccg aacagcgtcg agatgtactg cacgccgaag acctgcgaga tcagtccgct
     4321 ggtcagtggc accgtgccca gccacaggaa gcccatgccg aacgcaaaaa catagactga
     4381 gagctcgctg agcggcaatg cgagaaacgc caccatcagc gccgagcgcg ccagatacac
     4441 ccaggccagc agatgcttgc gccggtacac atcgccccag cgcccgaaca ggaaggtgcc
     4501 ggccacgttc gccagcgcga tcagcgacag cgcgatcacg gccgcgcgcg cgttcatccc
     4561 gtggtcggcc aggtaggcgg gcaagtggcc gccgatgaat gccaactgga agccgcaagc
     4621 gaagaagccg agattgagca gcaaaaagcc gcgatgacgg aatgcctcgc cgattgcgct
     4681 gcccatgccc tgtcttgccc ccggcgcgac cgtggccgac ggattgtcgg tcagcgtgaa
     4741 cgccagcggc gcaagcgccg cgatcgcaag ggccaggatc accagcgcgg ccatccatcc
     4801 gaagccgtcg atgaagccgt gcgcgaccgg tacggcgacg aactgcccga gtccgccgac
     4861 cgcgccggcc agtcccagtg cccatccgcg ccgctccggc ggcaccatgc gcgccagcgc
     4921 gccgtagacc gtgccaaagg cggtggccga caaggccatg ccgatcgcga ggccgccgga
     4981 cagggtcagc gccagcggcg tggcggcgtg cgccatccaa aacagaccga ggccatacag
     5041 caggcagccc gcaaagatca cccgcaacga accgaagcga tcggcgatca tgccggtgaa
     5101 cggctgggca agtccccaca gcaggttttg caacgcgatc gcgaatgcga attcctcgcg
     5161 cgaccagccg cgctcggccg tcatcggcag caggaacagc ccctgtccgt ggcgtatgcc
     5221 gagtgcaaga cccatgatga tgccgccaca gagaatcgcg gccagcggat tgatgcgcag
     5281 cgtgttcatg catgctcccg gggaatgacg tccttccagt gtagggagcg caatctgcgc
     5341 gacaagcgag atttgtgcgg gcgtcgcatg cgtgcgccgc atgcgtaccg atgctttcac
     5401 cagcacagtc ccggccgatt tggatgagcc gtaccgtggc gagctgccgc cgctgcgcta
     5461 cgatgcacat ctgcccactc gccttcacct gcacccatgc caagggaata tacgttccgt
     5521 atcgtcaatg tcttcgccga ggaaaaattc ggcggcaatc cgctttgcgt gttcgaggac
     5581 gggcgcggtc tcagcgacgc cgagatgcag gcgctggcgc gccagttcaa cctgtcggag
     5641 acgaccttca tcctgccgtc cgaatgcgcg gatgcgcggg tgcgcatctt cacgccgggt
     5701 tatgagctgc cgttcgccgg ccaccccacg ctgggttcgg cccacgtggt gcgcgccgcc
     5761 ggcgacggtg gcgatgccgt ctcgctcgag ttcaaggccg gcgtcgtgcc ggtgcgcgcg
     5821 aaaggcgacc tgtggtcctt caccgcgccg tccctggccg ccggcgccgt ccccgtctac
     5881 ccgccgaaag agccgcgcgc gctgatcgcc gcgctggtcg gcctgaacga aagcgacctg
     5941 gcggccgatc cggcatgggt gcacgccggt gtcgaccaac ttctgattcc ggtccgggat
     6001 gccgggacgg tgcggcgagc ggcgccggac agttctctgc tcgaccgctg gccgcagagc
     6061 agcctggggc gcaagaccgg gtacgtcttc gcgttcaccg gtgatgcggt gctggcccgg
     6121 tatttcttca ccacccaggg cggcggtgtg agcgaggacc cgggcaccgg gtccgcctgt
     6181 gcaaatctcg gtgcatggat gcggcacggc ggccgccagc cgccgttttc ggtacgcgtg
     6241 agccagggtg aagcggtgca gcggccgtgc gtgctgtacc tcgacgtgaa tgcgagcggg
     6301 cagatttcag taggcgggac ggtgcgcgaa ctcgggcgcg gcacgatcac gttgtaaggg
     6361 ggccatgctg ccaaggcatg gccttctgtg cgattgtcat ttgacgcggt ggcagctgcc
     6421 cggagatact gccccttcct cgtttaccgc tctcctccat gcagtccttg tgggttcttg
     6481 ccgcaacctt cctgttctcc atcatggggg tctgcgtcaa gctggcagcg gaccagcacg
     6541 gactgggtga aatcctgttt taccgcggcg cgatcggcgt cttcgtcatc ctgctgatct
     6601 gccgccttcg tttcgcgccg ctgcgcacgc cgcatctcgg gctccacttc aagcgcgggg
     6661 ccgtcggcct gatctcgatg tcgctgtggt tctacacgat cgcggtactg ccgctgccca
     6721 cggcgatgac gctggcctac acctcgccgc tctgggtggt cgcattcctg ctcgtggctg
     6781 ccgt
//
LOCUS       DATLGF010000351         6141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_182303, whole genome shotgun sequence.
ACCESSION   DATLGF010000351 DATLGF010000000
VERSION     DATLGF010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182303"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /gene="motB"
                     /locus_tag="VM406_10785"
     CDS             <1..925
                     /gene="motB"
                     /locus_tag="VM406_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HVL76489.1"
                     /translation="PIIVKRIKKGGGGHHGGAWKIAYADFVTAMMAFFLLMWLLGSTT
                     KGDLQGIADYFNTPLKVAMMGGSGSGDSSSVIQGGGKDLTRKDGQVRKADDPELRKTY
                     NLQAAHMRQIEKELERQENARLRALKAHIEAMIEARPTLRQFRSQLLLDITTEGLRIQ
                     IVDEQNRPMFALASAELQPYTRDILHEIGNALNEVPNKISLSGHTDATPYASGERRYS
                     NWELSADRANASRRALVAGGMDESKVLRVVGLSSAVLFDNENPFNPINRRISIIVMNK
                     KAEEAVTKEAPAVDAPGQADAELEGAPAAAQ"
     gene            1002..1547
                     /locus_tag="VM406_10790"
     CDS             1002..1547
                     /locus_tag="VM406_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HVL76490.1"
                     /translation="MTKEKRLGTHVRSLLSNLSGHGTQHLNEVETDLMQTSYLLGEAI
                     EKLGASFMAIHEAVSAQQASIDALLAAGQASPELVEQLRERHAEIGQHVNAAVTGLQF
                     QDLTNQLIGRTVRRVAGLREVLGGIGTGSSGISDTNAEEIVSALASINEVLEEQSVKL
                     ESALWKAVCQTHMESGDIELF"
     gene            1606..1971
                     /locus_tag="VM406_10795"
     CDS             1606..1971
                     /locus_tag="VM406_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL76491.1"
                     /translation="MAKTILAVDDSGSLRQMVVFSLRAAGYQVTEAVDGQDGLEKART
                     QVFDLVLTDQNMPRMDGLSLIRSLRTLPTYQKVPILMLTTESSDEMKSKGRAAGANGW
                     LVKPFDPQRLTEVVKKVIG"
     gene            2014..4203
                     /gene="cheA"
                     /locus_tag="VM406_10800"
     CDS             2014..4203
                     /gene="cheA"
                     /locus_tag="VM406_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HVL76492.1"
                     /translation="MTIDMSQFFQVFFDETEELLAEMEKLLLAIDVDAPDAEDLNAIF
                     RAAHSIKGGAGTFGLTDLTEVTHVLESLLDRIRKGEMTLTSEHVDAFLVAKDVLKMQL
                     DGHRLHTEVDQEAVADVRMMLQSLSQGDVPAAAMASLDGTPVSDPEPVGSGSVGVSHQ
                     FRIELPAVAERDVQALEAELGLLGPIRSEPLADGRTAFTLHSSGSRDDIVAICSFILD
                     PDQLAITEQSVPMAAEGAARKAAQEEAQGYGFFEPLEANPGQQAAAKAEQEARQGYGF
                     FDNFETAEMRAAREGGSAAPVEAAPPNVSDKPGSMPAVERKPAAGKRDSERGAATNAE
                     STSIRVSIEKVDQLINLVGELVITQAMIEQRTGSLDPMLHERLLSSVSQLTRNTRDLQ
                     EAVMSIRMMPMDYVFSRFPRMVRDLASKLGKRVDFVTHGAATELDKGLIERIVDPLTH
                     LVRNSIDHGIEMPEVRRAAGKSETGRLSLSAGHQGGNIVIEVSDDGGGLNRERILAKA
                     RAQGLPVSDTMPDSEVWQLIFAPGFSTAEVVTDVSGRGVGMDVVKRNITAMGGVVEIR
                     SAKGFGTTISISLPLTLAILDGMSIKVGEEIYILPLGYVVESLQPDPADVKEITGQGR
                     VIKVRGEYLPLIPLYQMFGIEPRFTNPSEGIVVILESEGRKAALFIDDLVGQQQVVVK
                     NLESNYRKVAGISGATILGDGGVSLIIDVAALLRSSRQLSDEPTYTR"
     gene            4215..4718
                     /locus_tag="VM406_10805"
     CDS             4215..4718
                     /locus_tag="VM406_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HVL76493.1"
                     /translation="MNQTTLQAGAADGERDMTGNEFLAFKLGKEEYGIDILKVQEIRG
                     YETVTRIANAPEFIKGVVNLRGIIVPIVDMRIKFNLGTPTYDQFTVVIILNIAGRVVG
                     MVVDSVSDVITLAPEQVKPAPEMGTALNTDYLIGLGTLDQRMLILVDIDRLMSSAEMG
                     LIDKIAA"
     gene            4804..>6141
                     /locus_tag="VM406_10810"
     CDS             4804..>6141
                     /locus_tag="VM406_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HVL76494.1"
                     /translation="MISLSNVKIGARLGLGFGLVLVCASLLLALGLWRMSDLHGTTQR
                     IVTEDVASLNAALEMREAGWSLALALRRIAAPTDATEGEREAKRLDAILENYEKSETL
                     LKKLSTDAATQAVLAPVLAQREEVIKIAQNIRKEAVGGNYFDAASLLKSDFSPVHEKW
                     IGSLIALAEHQQRNMRSTYAESEANYSTTRLGMLGFGLLTLALGALVAWFTTRTITVP
                     LQRAAAIADTIASGDLTSTIEQGGMDEAGQLVNSLKSMQGNLVNTVNRIKQGTETISV
                     ASREIASGNANLSARTEAQASSLEETASSMEELTTTVKQNAENARQANQLVVSASDHA
                     LKGGRVVGQVVDTMGSIKESSRKIVDIIGVIDGIAFQTNILALNAAVEAARAGEQGRG
                     FAVVAAEVRSLAQRSAGAAKEIKALIGDSVEKVDAGGRLVDEAGKTMDEIVNSV"
BASE COUNT         1251 a         1832 c         2067 g          991 t
ORIGIN      
        1 gcccatcata gtcaaacgga tcaagaaggg cggcggcgga catcacggcg gcgcctggaa
       61 gatcgcgtac gcggatttcg tgaccgcgat gatggcgttc ttcctgctca tgtggctgct
      121 gggctcgacc accaagggcg atctgcaggg tatcgccgat tatttcaata cgccgctgaa
      181 ggtcgcgatg atgggtggct cgggttcggg cgacagttcg tccgtgatcc agggcggcgg
      241 caaggatctg acccgcaagg acggccaggt gcgcaaggcg gatgatccgg agctgaggaa
      301 gacctacaac ctccaggccg cgcacatgcg ccagatcgaa aaggagctgg agcgtcagga
      361 aaacgcccgg ctgcgcgcgc tcaaggcgca catcgaggcg atgatcgagg cgcgtccgac
      421 tttgcgccag ttccgcagcc agctcctgct cgacattaca acagagggct tgcgcatcca
      481 gatcgttgac gagcagaatc gacccatgtt tgcgcttgca agcgcagagc tgcagccata
      541 cacccgcgac atcctgcacg agatcggaaa cgcgctgaac gaagtcccga acaagatcag
      601 cctgtccggc cataccgacg cgacgcctta tgcgagcggg gagcgcaggt acagcaactg
      661 ggaattgtcg gcggatcgag ccaatgcctc gcgcagggct ctggtggcag gcggaatgga
      721 cgagagcaag gtcctgcgcg tggtcggcct gtcctctgcg gtcctgttcg acaacgagaa
      781 tccgttcaat ccgatcaacc gccgcatcag catcatcgtg atgaacaaga aggcggaaga
      841 ggcggtgacg aaggaggcgc cggcggtcga cgcgccgggc caggcggatg cggagctgga
      901 aggggcacct gcggcggcgc agtaggacag acgtggtcag cggagaacgg cttccctgac
      961 aggtccatgg cggcgcgcaa ggaggggagg gtggttcgca tatgacaaaa gaaaaacgac
     1021 ttgggacgca cgtcaggagc ctcctgtcca atctgtccgg acatggcacg cagcacctga
     1081 acgaggtcga gaccgatctg atgcagacca gctatctgct cggtgaagcc atcgagaagc
     1141 tgggggcgag tttcatggcg atccacgagg cggtgtcggc gcagcaggca tcgatcgatg
     1201 cgctgctggc cgccggtcag gccagccccg agctggtcga gcagctgcgc gagcgccacg
     1261 cggaaatcgg ccagcacgtc aacgcggcgg tgaccggcct tcagttccag gacctgacga
     1321 accagctgat cgggcgcaca gtgcgccggg tggccggcct gcgcgaagtg ctgggcggca
     1381 tcggcaccgg cagctcgggc atttccgata ccaatgccga agagatcgtc agcgcgctgg
     1441 caagcatcaa cgaagtgctc gaggagcaga gcgttaagct ggagagcgca ctgtggaagg
     1501 ccgtgtgcca gacccacatg gaaagcggcg acatcgagct tttttagaat tcaatgaaga
     1561 gcgggcgcca gcccgcgtcc aacaagaaac agtggagtgt gaacaatggc gaaaacgatc
     1621 ctggcagtgg atgattccgg ttccttgcgg cagatggtgg tgttcagcct gcgtgccgcc
     1681 ggctatcagg tgaccgaggc ggtggacggt caggatggac tggaaaaggc acgcacccag
     1741 gtgttcgatc tggttctcac cgaccagaac atgccgcgca tggacggact gagcctgatc
     1801 cgctcgctgc gcaccctgcc gacctatcag aaggtgccga tcctgatgct gaccacggag
     1861 tcgagcgacg agatgaaatc gaagggacgt gccgccggcg cgaacggatg gctggtgaag
     1921 cccttcgatc cccagcgact gacggaagtc gtaaagaagg tcatcggcta gcggagccgt
     1981 cgacagaata agaacgagcc gagggagccc accatgacca ttgacatgag ccagttcttc
     2041 caggtcttct tcgacgagac cgaagagctt ctcgccgaga tggaaaagct gctgctggcc
     2101 atcgacgttg atgcgcccga tgcggaagat ctgaacgcga ttttccgcgc cgcgcattcc
     2161 atcaagggcg gcgccggcac cttcggcctg accgatctga ccgaagtgac ccatgtgctc
     2221 gaatccctgc tcgacaggat ccgcaagggc gagatgacgc tgacctcgga gcatgtggat
     2281 gcgttcctgg tcgccaagga cgtgctgaag atgcagctcg atgggcatcg cctgcatacc
     2341 gaagtcgacc aggaggcagt ggctgacgtg cgcatgatgc tgcagtcctt gtcgcaggga
     2401 gacgtgcccg cagcggccat ggcgtcgctc gatggtacgc cggtcagcga tccggagccc
     2461 gtcggctcag ggagcgtcgg cgtgtcgcac cagttccgca tcgagcttcc cgccgtcgcg
     2521 gagcgcgatg tgcaggcgct ggaggccgag ctcggtctgc tgggaccgat ccgcagcgag
     2581 ccgctcgcgg acggacgcac ggcctttacg ctgcacagct cgggctcgcg cgacgacatc
     2641 gtggcgatct gttcattcat cctcgatccg gaccagctcg cgatcaccga gcagagcgtt
     2701 ccgatggcag cggaaggggc ggcgcggaag gctgcacagg aagaggccca gggatacggc
     2761 ttcttcgagc cgctggaagc caacccgggg cagcaggccg cggcgaaggc cgaacaggag
     2821 gcgcgccaag gctacggctt cttcgacaac ttcgagactg ccgagatgcg tgcggctcgc
     2881 gagggtgggt cggccgcccc cgtcgaagct gcgccgccga acgtctccga caagccgggc
     2941 agcatgccgg ccgtcgagcg caagcccgcc gccggcaagc gcgatagcga gcgcggtgcg
     3001 gcgacgaacg cggagtcgac ctcgattcgg gtgagcatcg agaaggtcga ccagttgatc
     3061 aacctcgtcg gcgaactggt gatcacccag gcgatgatag agcagcgcac cggctcgctc
     3121 gatccgatgc tgcatgagcg cctgctgagc agcgtgagcc agctcacgcg caatacgcgc
     3181 gatctgcagg aagcggttat gtccatccgc atgatgccga tggactatgt gttttcgcgg
     3241 ttcccgcgca tggtgcgcga tctcgccagc aagctgggca agcgggtcga tttcgtcacg
     3301 cacggcgcgg ccaccgaact cgacaagggg ctgatcgagc gaatcgtcga tccactgacg
     3361 cacctggtgc gcaacagcat cgaccatggc atcgagatgc ccgaggtgcg gcgcgcggcg
     3421 ggcaagagcg agaccggtcg gctgtcgctt tcggccggtc accagggcgg caacatcgtg
     3481 atcgaggtca gcgacgacgg cggcggcctg aatcgcgaac ggatacttgc aaaggcgcgc
     3541 gcgcagggcc tccctgtgtc ggacacgatg cccgattccg aagtgtggca gctgatcttc
     3601 gcgccgggtt tctcgaccgc ggaagtcgtg accgatgtat cgggccgcgg cgtgggcatg
     3661 gatgtcgtca agcgcaacat cacggcaatg ggcggggtcg tcgagatccg ttcggccaag
     3721 ggtttcggca ccacgatttc gatttcgctg ccgctcactc tggcaatcct ggacggcatg
     3781 tcgatcaagg tcggcgagga aatttacatc ctgccgctcg gctatgtggt cgagtcgctg
     3841 cagcccgatc cggccgacgt caaggaaatc accgggcagg ggcgcgtcat caaggtgcgc
     3901 ggcgaatacc ttccgctgat tccgctctat cagatgttcg gcatcgagcc gcgcttcacc
     3961 aacccgtccg aaggcattgt cgtcatcctt gaatccgagg gcaggaaggc agcgctgttc
     4021 atcgacgacc tggtggggca gcagcaggtt gtggtgaaga acctcgaatc gaactaccgc
     4081 aaggtggccg gcatttccgg cgccaccatt cttggcgatg gcggcgtctc cctgatcatc
     4141 gatgtggctg cgctgctgcg ctcgagcagg cagctcagcg atgaacctac ctatacccga
     4201 taaggaacac ttccatgaac cagacgacac tccaggcggg cgcggccgac ggcgagcgcg
     4261 acatgaccgg caatgaattc cttgccttca agctcggcaa ggaggaatac ggcatcgaca
     4321 tcctgaaggt gcaggaaatc cgcggctacg aaacggtgac gcgcatcgcc aacgcgccgg
     4381 aattcatcaa gggcgtcgtg aatctgcgcg gcatcatcgt gccgatcgtc gacatgcgca
     4441 tcaagttcaa cctcggcacg ccaacctacg accagttcac ggtcgtgatc atcctgaaca
     4501 ttgccgggcg cgtcgtcggc atggtggtcg acagcgtgtc cgacgtgatc acgctggcgc
     4561 cggagcaggt caagccggcg ccggaaatgg gcaccgcgct caacaccgat tacctgatcg
     4621 gcctgggcac gctcgaccag cgcatgctga tcctggtcga catcgaccgc ctgatgtcca
     4681 gcgccgaaat gggcctgatc gacaagatcg ccgcatagcg gcaggctaat ccgatagtgg
     4741 ttgcacacgg tacgaaacaa ggaagtcccg catgaagcgg tcttttcaaa cagggagtga
     4801 aacatgatca gtctgtcgaa cgtgaagatt ggggcgcggc tcggactcgg tttcggtctc
     4861 gtgctcgtgt gcgcgtcgct gctgctcgct ctgggcctgt ggcgcatgag cgacctgcat
     4921 ggcacgacgc agcgcatcgt gaccgaggat gtggcaagcc tgaacgccgc gctggaaatg
     4981 cgcgaggcgg gctggtcgct cgctctcgcg ctgcggcgca tcgccgcgcc caccgatgcg
     5041 acggagggcg agcgtgaggc caagcggctc gatgcgatcc tcgaaaacta tgaaaaatcc
     5101 gagacgctgc tgaagaagct gtctaccgat gcggccacgc aggcggttct tgcgccggtg
     5161 ctggcgcagc gcgaagaagt gatcaagatc gcgcagaaca tccgcaagga agcggtcggc
     5221 ggaaactatt tcgacgccgc ttcgctgctc aagagcgatt tctcgccggt gcacgagaag
     5281 tggatcggca gcctgatcgc gctggccgag caccagcagc gcaacatgcg ctccacctac
     5341 gcggaaagcg aagccaacta ttccaccacg cgcctgggca tgctcggctt cggcctgttg
     5401 acgctggcgc tgggtgcgct ggtggcctgg ttcacgacgc gcacgatcac cgtgccgctg
     5461 cagcgcgcgg ctgcaatcgc cgatacgatc gccagcggcg acctcacctc gacgatcgag
     5521 cagggcggca tggacgaggc ggggcagctt gtcaattcgc tcaagagcat gcagggcaat
     5581 ctcgtcaaca cggtgaaccg catcaagcag ggcaccgaaa cgatatctgt cgcatcgcgc
     5641 gagatcgctt ccggcaacgc gaacctgtcg gcgcgcacgg aagcgcaggc gagctcgctg
     5701 gaggagacgg catcctcgat ggaggagctg accacgacgg tcaagcaaaa cgccgagaat
     5761 gcgcgacagg cgaatcagct tgtcgtctcc gcctccgacc acgcgttgaa gggcggccgg
     5821 gtggtcggac aggtggtcga cacgatgggc tcaattaagg agagttcgcg caagatcgtg
     5881 gacatcatcg gggtgatcga cggcatcgct ttccagacca acatcctggc tttgaacgcc
     5941 gcggtcgaag cggcgcgggc gggcgagcag ggacgcggct tcgcggtcgt ggctgcggaa
     6001 gtccgcagcc tggcgcagcg ctcggccggc gcggcgaagg agatcaaggc actgatcggc
     6061 gattcggtcg agaaagtgga cgcgggcggc cgcctggtcg acgaggccgg caagacgatg
     6121 gatgagatcg tcaattcggt c
//
LOCUS       DATLGF010000352        13570 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_213447, whole genome shotgun sequence.
ACCESSION   DATLGF010000352 DATLGF010000000
VERSION     DATLGF010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213447"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="VM406_10815"
     CDS             <1..1314
                     /locus_tag="VM406_10815"
                     /inference="COORDINATES: protein motif:HMM:NF019236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HVL76495.1"
                     /translation="PAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPTGPTYYISPNGND
                     SNPGTMSAPWRTIQKAANTLVAGDTAVLLNGVYEEGSIRFARSGTASKRITIKAQNKW
                     QAVVSSTSGCQPGFSIAASYITVQDLRFSVSPRNATCGNYTSTNVAIRAWDLHNPNPS
                     NPNSGYVGFVADGIKVDAGLQRSEGVKSNQDFTIIQNSEFGNTIELFNTKDSIIRNNV
                     VTNQNLWGNSIFVKGGTRNTQIYNNTIYNKFRNGYALYLGGSTGDPWHFDGNTKFEAY
                     NSVAYNNVIVNESGGGMVGLIFSGARDSAFYNNVVVGGSITMMQGGGSGTRASTTNPK
                     IQNNIIICHANSAALTGSHQGTMTISNNNFSNCGSVPNQASPVTGDPLLVNRTSDWRL
                     QTNSPARNTGTTVSLTGFDGKPIDVSRDRNGVVRTAPWDLGIYNY"
     gene            complement(1376..3388)
                     /gene="asnB"
                     /locus_tag="VM406_10820"
     CDS             complement(1376..3388)
                     /gene="asnB"
                     /locus_tag="VM406_10820"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HVL76496.1"
                     /translation="MCGLTGFLRAAPCAAEEQLAVLGRMTDAIEHRGPDDAGHWSDGA
                     AGIFLGHRRLSIVDLSPAGHQPMRSASGRYLIVYNGEIYNHLQLRAQLPADLPWRGHS
                     DTETLLAGFDAWGIRGTTERLIGMFAFAVWDRQAATLTLARDRIGEKPLYYGWQGRGR
                     DAVFLFGSELKALRAHPAFVGVVNRDALALQMRHNYIPAPYSIYEGIAKLPPGSLLRL
                     AHGQREPVIEAYWSVARAVELGMADPFIGTPEEAVDALEHLLKDAVHAQMAADVPLGA
                     FLSGGVDSSTVVALMQRQSVQPVKTFSIGFHEAGFNEAHHAKAVAQHLGTDHTELYVS
                     AEQVRDVIARMPLVYDEPFSDSSQLPTFLVAQLARQHVTVSLSGDAGDELFCGYDRYS
                     ITARTWKLMNATPLAMRRMAAKGLTAVRASTWSRLADGAARVLPPPMRIPNVGDKLHK
                     GAYALQCQSLDALYLRLVSHWDDPASMVIGATEPRTVLTAPDERFVKLDGIQRMMALD
                     MLSYLPDDILVKVDRAAMSVSLETRVPFLDHRVVELAWRLPLSFKLREGTTKWALRQV
                     LYRYVPKTLIERPKMGFGVPIGDWLRGPLRGWAENLLDAARLRRDGYFDSDMVRQRWD
                     EHLSGRRNWQYHLWDVLMFQSWHEEQERGIERGLGRSAVPRYAARA"
     gene            complement(3388..4554)
                     /locus_tag="VM406_10825"
     CDS             complement(3388..4554)
                     /locus_tag="VM406_10825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVL76497.1"
                     /translation="MPTALPSGRPAQMRHVLHVVSGLRVGGAEMALYRLVLHSRGSGY
                     IHTVVALTPEGDMAERFRSSGVELIVLDFRRSPVSQYLRLLRLMRLRRPDIVQTWLYH
                     ADLFGGLAARLAGIRNVIWGVHTTYVTGGPRATAAVRWLCGRLSRWVPSTIVCVAEAA
                     RREHVRIGYDAGRVVVVPNGFDLSFLVAGDGERERLRRECGFAEDDVVIGTVGRFSPD
                     KDHGNFIQAAALLARRNASLRFLMVGKGLEPGNAVLMNWIRESGCAKRFVLLGERHDI
                     PACLAGMDVFCLSSRTEAFPLVVGEAMAMGLPCVSTDVGDVSFLLGGAGIVVPKENPE
                     AMAQAVAQLLSLEPASRRKLGEQASARIRAEFAMERARERIEGIYETVLQRRSV"
     gene            complement(4608..5945)
                     /locus_tag="VM406_10830"
     CDS             complement(4608..5945)
                     /locus_tag="VM406_10830"
                     /inference="COORDINATES: protein motif:HMM:NF019236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HVL76498.1"
                     /translation="MRVQKIRTAAAAGVLVLSSCGGGAAADGVDVQVGAARTYYVSPR
                     GSDTNPGSVDEPWRTIQKAADTLGPGDTAVLLDGNYEEGMVQFTRSGTQSDRITVRAQ
                     NKWGAVVRSTSGCDPAFSIRASYITVQGLRFSVSENNGGCGSYTSTNVAIRAWEEMNP
                     RPSQPDSGHVGFIADGIKVDADSARSEGVKSNQDFTVIQNSEFGNSIELFNSKNSVIR
                     NNVVNGQNLWGISIFVKGGVRNAQVYNNVIYNKNRNGYGIYLGGVTADPWHFDETARF
                     EAYHSVAYNNVVVNESGGSNMRALILAGALNSAFFNNVVVGGSLAMMEGGPESGPRAA
                     TTNPRFENNIVICHASGPALTGSYLGTLTIGNNNFAGCSDAPAQANPVTGDPLLLNRS
                     SDWRLRAGSPALNAGSVITFIGFDGMPIDVSRDRNGVARTAPWDLGVYEQSDQ"
     gene            complement(5926..7242)
                     /locus_tag="VM406_10835"
     CDS             complement(5926..7242)
                     /locus_tag="VM406_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVL76499.1"
                     /translation="MGTRSDGLSRKAARPARGRRCQLALLPSWHRHTPGNGELTMNRT
                     IMLSSNTAWSIVNFRSGLIRHLQSDGYSVVAVAPHDAHAPRLIDLGCRFIDLPMDNHG
                     THPGRDMLLLARYLRLLKALRPSVYLGYTVKPNVYGSLAAHALRIPVVNNIAGLGSAF
                     VENNLLTRIVQALYKCSLRGSERVFFQNGEDRELFIRSGLVGADSTDRLPGSGVDLSR
                     YPARAPSVLHGRPFRFLLVARVLRSKGIVEYVEAARAVRRHVSNARFQLLGFVEKRDR
                     NAIPVEDIRAWEREGVLDYLGGTDDVVPFLAEADCVVLPSYYREGVPRALLEAAAMAR
                     PLIASDAVGCRDAVDDGINGFLCKPKNANDLAQKMLAMIRLDPRVRIHMGMAGRRKVE
                     AEFDERLVLNKYSDVIRAIEQNGRIRQGRQRTGSVSGGREYARTED"
     gene            complement(7125..8612)
                     /locus_tag="VM406_10840"
     CDS             complement(7125..8612)
                     /locus_tag="VM406_10840"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HVL76500.1"
                     /translation="MKITVIGTGYVGLVSGACLAEFGNDVFCLDVDRRKIDMLNRGEV
                     PIHEPGLSEIVRRNQQNGRLRFSTDVAAAVGHGDIQIIAVGTPPDEDGSADLKYVLAA
                     ARNIGRHMSGFKVIVDKSTVPVGTAEKVRQEVQAQLDARRAHVRFSVVSNPEFLKEGA
                     AVDDFIRPDRIVVGCESGPEGEMARELMRQMYAAFNRNHYRMLWMDVRSAEFTKYAAN
                     AMLATRISFMNELAMLAEKLEADIESVRQGIGSDPRIGYSFLYPGCGFGGSCFPKDVQ
                     ALERTARDCEHELLILRAVTQVNDRQKKVLARKIVSRFGEELNGRHFAVWGLAFKPNT
                     DDMREASSRVLLRELMLRGATVAVYDPVAMGEARRVLEGDLKDIPGAMDRIRFSADHL
                     DALDGAEALTIVTEWKDFRAPDFERIRMRLKSPVIIDGRNLYDPETVRQAGFEYYAIG
                     RAVRRMSWVPAATDFREKRRAQLGAAAVNSRFFRRGTDIRPGMEN"
     gene            complement(8680..9735)
                     /locus_tag="VM406_10845"
     CDS             complement(8680..9735)
                     /locus_tag="VM406_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVL76501.1"
                     /translation="MPTDTILVTGAAGFIGSHVAARLAEMGLRTIGCDNFNRYYSPRL
                     KADRVRTLLATRGAACLTVELADPEQVERLFRRVRPGRVLHLAAQAGVRYSLQHPQAY
                     INANLVAFANVMQACQRHGVEHFVYASSSSVYGANSKVPFREDDRTDEPVSLYAATKK
                     ANELLAHSYSMIHGMPSTGLRFFTVYGPWGRPDMAYFDFTKKMLNGERVQVFSNGQLR
                     RDFTYIDDIVEGVVRLLLHPPRAEQGAAPHAVYNIGNHQPVTVMEFLATLEKVLGVRA
                     MVDFVPMQPGDVPATFADTSRLRERVQFAPDTPLESGLARFVDWYRSWSTESEIRPAP
                     TSRSMDLKGKRLGSLMQ"
     gene            complement(9839..11581)
                     /locus_tag="VM406_10850"
     CDS             complement(9839..11581)
                     /locus_tag="VM406_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76502.1"
                     /translation="MVDGTTLPAWQARALEAIRRDGLVELALVVRACGVAPDDATAGR
                     VCQTGWRSLLARAWLHLDRKRFGGRRGALQAVDAAALLHGVPAIDVRAETDGGFDVIV
                     PADAPAVADYRLDVLLRFSFRALRGKICDAARCGVWYYGCGEDATFQCPLPAVWEVLL
                     RQPTTATDLLMVAGGGADVAVLERSWSPTDTISPARTLDRMLWKCASYAPRRLRELGR
                     LGPDAFLHRYRPARPEPVDRGQVLPPIPGNLAVLRGLGTRVAEKLAARVRNLVFFDQW
                     MLMYRITDVEQPAKAGPEFTPLVPPKDRFWADPFVVQYQGQYAVFIEECEYADSTGYL
                     SVIGFNRDGAPLLPPRPIMKRPYHLSYPFVFEDGGTLYMIPESAENEAISIYRCRSFP
                     DEWEFVMNLLERVTAYDSTIWRHNGRYWLFATVCENREASSWDEVFLYHSDSLLSRNW
                     IAHPCNPIVSDVRCARPAGRIFEHRGAWYRPAQDCSRRYGGAIDVRRIEAIDEHTYRE
                     VSAGRIEADWHAGLSGIHTLNHAGRLTCIDVIRRRPKWPAARVTRMDSVFDSLAKAKR
                     APSTLPARQAPYRQ"
     gene            complement(11637..12899)
                     /locus_tag="VM406_10855"
     CDS             complement(11637..12899)
                     /locus_tag="VM406_10855"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HVL76503.1"
                     /translation="MQDSEARRARPVLYYGSRAVPRATTALLWNGYRTYIGVVLSLFL
                     LVLFLNLPNYGIALNASLPPKLWYFAFVLLLAPLFFQTRALLFYLFSPFALWALAYVL
                     INLIHLLGGVSGGEVARDAAILTRIQFVVLAVALGFALWSWRDFGYDGIFRAVAVLAP
                     VVVILDFLVPGLFYPPGTPGTVPGRASATFINPTIAGEVILITFLFACPSVSMRYRMP
                     LFLLTGLGAAMTFSRSAIVAWMVLWAFLQYRRVLPAAGYAYVLAFCAAIPFVLAALQG
                     YLGSRADLDGAMGNLQDRISFFSNPSFNDESSQERYEALRAGLELFLQSPIEGAGAGA
                     TSSWLLAVGTHNQFILLAAEYGVVGIACWLAMARMLWTGKYFADRSLQIALVFLFVFL
                     SMFAHTVFDMLYWLITFALVSDRTSPER"
     gene            complement(13055..>13570)
                     /locus_tag="VM406_10860"
     CDS             complement(13055..>13570)
                     /locus_tag="VM406_10860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVL76504.1"
                     /translation="GRLSEEKNFDLLIDVFTRLAPRHPDWDLVILGEGPERAALEGKV
                     GIAGLRRRVFLPGRVGNMGRWYERADLYVMTSRFEGFPNTLVEAMSYGLPAVSFDCDT
                     GPRDIIRPGIDGLLIRPGDLAALEAALGSLMTGTSERQAFARAALCTRERFSVERISS
                     MWEQLFGQCAS"
BASE COUNT         2676 a         4427 c         4067 g         2400 t
ORIGIN      
        1 ccggcgcctg cacctgctcc ggcacccgct cctgctccgg cacctgctcc tgcacccgcg
       61 cccgctccgg cacccgcacc tgcgcccgca cccaccgggc cgacctacta catctcgcca
      121 aacggaaacg actccaatcc gggcacgatg tcagcaccgt ggagaacgat tcaaaaagct
      181 gccaacaccc tggtcgccgg cgataccgcg gtcctgctca atggcgtgta cgaagagggc
      241 tccatccgat ttgcccgctc cggcacggca agcaaacgca tcacaatcaa ggcgcagaac
      301 aagtggcagg ccgtggtcag ctccacctcc ggctgccaac ccggcttcag catcgccgcc
      361 agctacatca ccgtgcagga cctgcgcttc tcggtctcgc cgcgcaacgc aacctgcggc
      421 aactacacct cgacgaacgt ggcgatccgc gcctgggatc tgcataaccc gaacccgtcc
      481 aacccgaatt ccggctatgt cggctttgtc gcggacggca tcaaggtcga tgcgggactg
      541 cagcggtcgg aaggcgtgaa gtcgaaccag gacttcacga tcatccagaa ctccgagttc
      601 ggcaacacga tcgagctgtt caacacgaag gattcgatca tccgcaacaa cgtggtgacg
      661 aatcagaacc tgtggggcaa ctcgatcttc gtcaagggcg gcacgcgcaa cacgcagatc
      721 tacaacaaca ccatctacaa caagttccgc aatggttacg cgctttacct cggcggctcg
      781 acgggcgacc cgtggcactt cgacggcaac accaagttcg aagcgtacaa ctcggtggcg
      841 tacaacaacg taatcgtcaa cgagagcggc ggcggcatgg taggtctgat cttctccggc
      901 gcccgtgaca gcgccttcta caacaacgtc gtggtcggcg gttcgatcac gatgatgcaa
      961 ggaggcgggt ctggtacacg cgcatcgacg accaacccga agatccagaa caacatcatt
     1021 atctgccacg cgaattccgc ggcgctgacc ggcagccacc agggcacgat gacgatctcc
     1081 aacaacaact tcagcaactg cggcagcgtg cctaatcagg ccagcccggt aactggagac
     1141 ccgctgttgg tcaatcgcac gtccgactgg cgcctgcaga ccaacagccc ggcccgcaac
     1201 accggcacca cggtgtcgtt gaccggcttt gacggcaaac ctatcgacgt ctcgcgtgat
     1261 cgcaatggcg tcgttcgtac tgcgccgtgg gatctgggta tctacaacta ctaatccatc
     1321 cgaagtagcg caggtccggg aggcggcgat cgccgccgcc tggtcctgct gatcatcacg
     1381 cccgcgcggc atagcgaggc acggcgctgc ggcccaaacc tcgctcgatg ccgcgctcct
     1441 gctcctcatg ccaggactgg aacatcagca catcccacag gtgatactgc cagttgcgtc
     1501 ggccggacag atgctcgtcc cagcgttgcc tgaccatgtc cgaatcgaag tatccgtcgc
     1561 gacgcagtcg cgccgcatcc agaaggttct ccgcccagcc gcgcagcggc ccccgcagcc
     1621 agtctccgat cggcacgccg aatcccatct tcggccgctc gatcagggtt ttcggcacat
     1681 atctgtaaag gacctgcctg agcgcccatt ttgttgtccc ctctcgcagt ttgaacgaca
     1741 ggggcagacg ccaggcgagc tcgacgacac ggtggtccag gaacggcacg cgtgtttcca
     1801 gtgaaacgct catcgcagca cgatccacct tcacgaggat gtcgtcaggg agataactca
     1861 gcatgtcgag cgccatcatg cgctgtatgc cgtcaagctt cacaaaccgc tcgtcaggcg
     1921 cggtgagcac ggtacgcggc tctgtcgccc cgatcaccat cgaagccgga tcgtcccagt
     1981 gcgagacgag gcggaggtag agtgcgtcga gcgactggca ttggagcgcg tacgccccct
     2041 tgtgcagctt gtctccgacg ttgggaatcc gcatcggcgg cggaaggacg cgcgccgcgc
     2101 cgtcggcaag ccgcgaccag gtactcgcgc gcaccgcagt cagccctttt gccgccatcc
     2161 ggcgcatggc gagaggcgtt gcattcatca acttccatgt tcgcgcggtg atgctgtagc
     2221 ggtcatagcc gcagaacagc tcgtcccccg catcgcccga cagtgaaacg gtcacgtgct
     2281 ggcgcgccaa ctgcgcgacg aggaaggtcg gcagttgcga cgaatcggag aatggctcgt
     2341 cgtacacgag cggcatgcgt gcgatcacgt ccctcacctg ttccgccgac acatagagct
     2401 ccgtgtgatc ggtgcctaga tgctgcgcga ccgccttggc atggtgcgct tcgttgaaac
     2461 cggcttcgtg gaacccgatc gaaaacgtct tcaccggctg gacggattgc cgctgcatca
     2521 gggcgaccac ggtcgacgag tccaccccgc cggagaggaa tgctccaagc ggcacatcag
     2581 ccgccatctg tgcgtgcacc gcatccttga gcaggtgctc cagggcgtca accgcttctt
     2641 ccggcgtgcc gatgaacgga tccgccatgc cgagttcgac cgcccgggcg acggaccagt
     2701 aggcttcgat cacaggctca cgctgcccgt gcgcgagcct caaaaggctt cccggaggaa
     2761 gcttcgcgat gccttcgtag atcgagtacg gtgcgggaat atagttgtgc cgcatctgca
     2821 gggccagcgc gtcgcggttg acaacaccga cgaatgccgg atgcgcgcgc aaggccttca
     2881 gttcggagcc gaacaggaag accgcgtccc ggccgcggcc ctgccagccg tagtagagcg
     2941 gcttttcgcc gatccgatcg cgggcgagcg tcagcgtggc ggcctgccgg tcccagactg
     3001 caaacgcgaa catgccgatc aagcgctccg tcgtcccgcg gatgccccat gcatcaaacc
     3061 ctgcgagcag cgtttccgta tccgagtgtc cgcgccacgg aaggtcggcg ggcagctggg
     3121 cacgcagctg aagatgattg tagatttcgc cgttgtagac gatcaggtag cgccccgaag
     3181 ccgagcgcat tggctgatgt cccgccggcg aaagatcgac gatggacagg cgcctgtggc
     3241 cgaggaagat gcctgctgcg ccgtcggacc aatggccggc gtcgtccggg ccgcgatgct
     3301 cgatcgcatc ggtcatgcgt ccgaggaccg caagctgctc ttccgctgca cagggtgcag
     3361 cccgtagaaa accagttaac ccgcacatca gacgcttctc ctttgcaaga ccgtttcgta
     3421 gatgccttcg atacgctcgc gcgcccgctc catcgcgaac tcggcacgta tgcgggcgct
     3481 ggcctgctcg ccgagtttgc gtcgcgatgc aggctcaaga ctgagcagct gcgccacggc
     3541 ctgcgccatc gcctccggat tttccttggg aacgacgatc ccggccccgc ccagcaggaa
     3601 cgagacgtcg ccgacgtcgg tcgacacgca gggcagcccc atcgccatgg cttctccaac
     3661 caccagcggg aaagcctcgg tccgcgagga caggcagaac acgtccatgc cggcaaggca
     3721 ggccggtatg tcatgccgct ccccgagcag cacgaagcgc ttggcgcagc cggactcacg
     3781 tatccagttc atcagcacgg cattgccggg ctcgaggccc ttccccacca tcaggaaacg
     3841 caggcttgca ttccgcctgg ccagcaatgc ggccgcctgt atgaaatttc cgtgatcctt
     3901 gtcgggactg aaacggccca ccgtaccgat gaccacgtca tcctctgcga agccgcattc
     3961 gcgtcgcagg cgctcccgct ccccgtcccc cgccacgagg aaggagaggt cgaagccgtt
     4021 cggcaccacc acaacgcggc cggcgtcgta gccgatccgg acgtgctccc tgcgcgcggc
     4081 ctcggcgacg cagacaatgg tgctcggcac ccagcgcgag agccgtccgc acagccatcg
     4141 cacggcggcg gtggcgcgcg ggccgccggt cacgtaggtc gtatgaacgc cccatatcac
     4201 gttgcggatg cccgccagcc gtgccgcaag gccaccgaac aggtccgcgt gatacagcca
     4261 ggtctgcacg atgtccggcc gacgaaggcg catcagccgc agcaggcgca agtactgaga
     4321 gacgggcgag cgccggaagt cgagcacgat cagctccacg ccggaacttc gaaaacgctc
     4381 ggccatgtcc ccttccggcg tcaacgcgac cacggtgtgg atgtacccgc tcccacggga
     4441 atgcagtacg agtcggtaaa gcgccatttc cgcgccgccg acacgaagtc cggagacgac
     4501 gtgcagcaca tgacgcattt gcgccggtcg ccccgacggc aaagcggtcg gcatggaagg
     4561 aggcggtgcg ttttcttccg gctttgtgga tttgcgtgcc atggccgtca ctggtctgac
     4621 tgttcgtaca cgccgaggtc ccagggcgca gtcctcgcta cgccattgcg gtcgcgcgag
     4681 acgtcgatcg gcataccgtc aaagccgatg aaggtaatga cggatcccgc gttcaacgcc
     4741 ggactgccgg ctcgcaagcg ccagtcggat gaccggttga gcagcagcgg gtcgccggtc
     4801 accggattgg cttgcgcggg agcgtcgctg caaccggcga aattgttgtt cccgatcgtc
     4861 agcgtcccca gataacttcc ggtcagtgcg ggaccgctgg catgacagat cacgatgttg
     4921 ttttcgaaac gcggattggt ggttgccgca cgcgggccgg actcgggccc gccttccatc
     4981 atggcaagcg atccgccgac cacgacgttg ttgaagaagg cgctgttgag cgcacccgcg
     5041 agaatcagcg ccctcatgtt gcttccgccg ctttcgttca ccaccacatt gttgtaggcg
     5101 acggaatggt aggcttcgaa cctcgccgtt tcgtcgaaat gccagggatc ggcggtaacg
     5161 ccgccgagat agattccata tccgtttctg ttcttgttat agatcacgtt gttgtaaacc
     5221 tgggcattgc ggactccgcc cttgacgaag atggagatgc cccagaggtt ctgtccattc
     5281 accacgttat tgcgaatgac cgagttcttg ctgttgaaga gttcgatgga attgccgaac
     5341 tccgagttct ggatcactgt gaagtcctga ttcgacttca ctccttccga gcgtgcggag
     5401 tcggcatcca ccttgatgcc gtcggcgatg aaccccacat ggcccgaatc aggttgcgac
     5461 ggacgcggat tcatctcttc ccatgcgcgg atggccacat tggtcgacgt atagctgccg
     5521 cagccgccat tgttttccga gaccgagaag cgcagtccct gcacggtgat atagctggcc
     5581 ctgatgctga atgccgggtc gcaccccgat gtcgaccgga ccaccgcacc ccatttgttc
     5641 tgcgccctga cggtgatgcg gtcgctctgg gtgccagagc gggtaaactg caccatgcct
     5701 tcctcgtagt tgccgtcgag cagcaccgcg gtatcacccg ggcccaaggt gtcggcggcc
     5761 ttctgtatgg tccgccatgg ctcgtcgacg ctgcccggat tggtgtcgct gccgcgaggc
     5821 gaaacatagt aggtgcgcgc cgccccgacc tgcacgtcga caccgtccgc ggccgcgcct
     5881 cctccgcagg aggaaagcac gaggacgccc gccgccgcgg ccgtcctaat cttctgtacg
     5941 cgcatattca cgtcctcctg aaacgctccc ggttctttgt cgaccctgcc ggattcgccc
     6001 gttctgctcg atcgcgcgga tcacgtccga gtacttgttc agcacaagac gctcgtcaaa
     6061 ctccgcttcc accttgcgcc ttccggccat ccccatgtgg atgcgcacgc gcggatccag
     6121 ccggatcatc gcgagcatct tctgggcgag gtcgttcgcg ttcttcggct tgcagaggaa
     6181 tccgttgatg ccatcgtcga ccgcgtcccg gcacccgacg gcgtcggatg cgatcagcgg
     6241 ccgtgccatt gcggcggctt ccagcaaggc ccgcggcacc ccttcccgat agtacgaggg
     6301 caagaccacg cagtctgcct cggcaaggaa gggcacgaca tcgtccgtgc ccccgaggta
     6361 gtccagcacc ccttcgcgct cccatgcgcg aatgtcttca acaggaattg cgttgcggtc
     6421 gcgcttttcc acaaaaccta gcaactggaa acgcgcgttc gaaacgtgcc tgcgcaccgc
     6481 cctcgccgct tcgacgtatt cgacgatgcc cttgcttctc agtaccctgg cgacgagcag
     6541 gaaccggaat ggccggccgt gaaggacact cggcgcgcgc gcaggatagc gagagagatc
     6601 gacacccgag cctggcagac ggtcggtgga atccgcgccc accagtccgg agcggatgaa
     6661 gagctcgcga tcctcgccgt tctggaagaa cacgcgctca gacccgcgca aactgcattt
     6721 gtacaaggcc tggacgatgc gggtcagcag attgttctcg acaaaggcgc tcccaaggcc
     6781 ggcgatgtta ttgaccacgg gaatgcgcag cgcgtgtgcg gcaagcgaac catacacgtt
     6841 cggcttgacg gtgtagccca ggtagacgga cggccgcagc gccttcagca accgcagata
     6901 gcgggccaaa aggagcatat cccggccagg atgcgtgcca tggttgtcca tcggcaggtc
     6961 gatgaagcgg catcccaagt cgatgagccg cggggcgtgc gcatcatggg gcgcgactgc
     7021 aaccaccgag tagccgtccg attgcaggtg cctgatcagg ccggagcgga aattgacgat
     7081 gctccatgcc gtattggacg aaagcatgat ggtccgattc atggtcaatt ctccattccc
     7141 gggcgtatgt cggtgccacg acggaagaag cgcgagttga cagcggcggc cccgagctgg
     7201 gcgcgccgct tttcgcgaaa gtccgtcgct gcgggtaccc acgacatcct gcgcacggcg
     7261 cggccgatcg cgtagtactc gaagccggcc tgacgcacgg tctcgggatc ataaagattc
     7321 cgtccgtcga tgattaccgg ggacttgagg cgcatgcgta tccgctcgaa atccggcgcg
     7381 cggaaatcct tccattcggt gacgatggtc agcgcctcgg cgccgtccag cgcatcgagg
     7441 tgatccgcgc tgaaacggat ccggtccatt gctcctggaa tgtctttgag gtcgccctcc
     7501 agcacccgcc tggcctcccc catcgcgacc ggatcgtaga ctgcgacagt cgccccgcgc
     7561 agcatcagct cgcgcaacag cacgcgcgaa gaggcctcgc gcatgtcatc ggtgttcggc
     7621 ttgaacgcca gcccccacac cgcgaaatgc cttccgttaa gctcctcccc aaagcgcgat
     7681 acgatcttcc gggcgagcac cttcttctgc ctgtcgttga cctgcgtgac ggcgcgaagg
     7741 atcagcaatt catgctcgca atcgcgcgcg gtgcgctcca gcgcctgcac gtccttggga
     7801 aagcaggagc cgccaaaacc gcagccggga tagaggaagc tgtagccgat acgcggatcc
     7861 gaacctattc cctgccgaac ggattcgata tcggcttcga gcttctccgc cagcatggcg
     7921 agctcgttca tgaacgaaat gcgcgtggca agcatcgcgt tcgcggcata cttcgtgaac
     7981 tccgccgagc gcacatccat ccatagcatc cggtaatggt tgcgattgaa tgcggcatac
     8041 atctggcgca tcagttcgcg tgccatctct ccttcaggac cgctttcgca gccgaccaca
     8101 atgcggtccg gcctgatgaa atcatcaacg gctgcaccct ccttcaggaa ctccgggttt
     8161 gacaccaccg aaaaccgcac atgcgcacgg cgcgcatcca gctgcgcctg cacttcctgc
     8221 cggacctttt cggcggtacc aaccggcacc gtggacttgt cgacgatgac cttgaagcca
     8281 gacatgtggc ggccgatgtt tctggctgcc gccagcacgt acttcaggtc ggccgaccca
     8341 tcctcgtcag gcggagtgcc gacggcgatg atctgaatgt cgccatgacc aacggctgcc
     8401 gccacatccg tggagaagcg caggcgaccg ttctgctgat tgcgtctgac gatctcggac
     8461 aggcctggtt catgaatggg gacttcgccc cggttaagca tgtcgatctt gcgccgatcc
     8521 acgtccaggc agaacacgtc attgccaaat tccgccaggc aggctccgct gaccaggccc
     8581 acataccccg taccgatcac tgtgatcttc acgatatccc tctcttcggt tcttgtttcg
     8641 ttctcatcgc attccaggct gcgcaggcgc agcgacggct cactgcatga gactgccgag
     8701 acgctttccc ttcaggtcca tggaacgaga ggtaggcgca ggacggattt ccgactccgt
     8761 agaccatgac cggtaccagt cgacgaaacg tgcaagcccg ctttcaagcg gtgtatccgg
     8821 cgcgaactgc acgcgttcgc gcaggcggct cgtgtccgca aacgtggccg gtacgtctcc
     8881 cggctgcatc ggaacgaagt cgaccatcgc acgcacgccg aggactttct cgagggtggc
     8941 caggaactcc atcaccgtta ccggctggtg gttcccgatg ttgtagaccg catgcggcgc
     9001 agcaccctgc tctgcgcggg gaggatggag cagcaggcga acgacgcctt cgacaatgtc
     9061 atcgatatag gtgaagtcgc gccgcaactg cccgttcgaa aacacctgga cgcgttctcc
     9121 attcagcatc ttctttgtga aatcgaagta ggccatgtcc ggcctgcccc atggaccgta
     9181 aacggtaaag aagcgcaggc cggtcgaagg catgccgtgg atcatgctgt aagagtgagc
     9241 aagcagctcg ttggccttct tggtcgctgc atacagcgag accggttcat cggtccgatc
     9301 gtcttcacgg aacggtacct tgctgttggc tccgtacacg ctgctgctgc tcgcgtagac
     9361 gaagtgctcg acaccgtgcc gctgacacgc ctgcatgacg tttgcgaacg caacgagatt
     9421 ggcgttgata taggcctgcg ggtgctgcag cgaatagcgt actccggcct gggcggcaag
     9481 atgcagcacg cggcctggac gcacgcgccg gaacaggcgc tcgacctgct cgggatcggc
     9541 gagctcgacg gtcaggcatg cggcgccgcg cgtggccagc agtgtcctga cacggtccgc
     9601 cttgagccgc ggagagtaat agcgattgaa gttgtcgcat ccaatcgtgc gcagccccat
     9661 ttcagccagg cgcgcggcaa catggctgcc gatgaagccg gcggcgcccg tgaccagaat
     9721 tgtgtcggtc ggcattgttg ttcctccatg acgagttgtc tgcatacagg cggctatgca
     9781 ggagtgcgtg gtggcgatac ggtttgcagg aatcgggcgg cgagcacggc ggtcggagct
     9841 attgtcgata cggggcttgc cgcgcaggga gtgtggacgg tgcccgcttc gcctttgcca
     9901 gggaatcgaa gacggaatcc atccgggtga ctctggctgc cggccatttc ggccgtcgcc
     9961 tgatcacgtc gatacaggtc aggcggccgg cgtggttcag cgtatgaatg ccggacaagc
    10021 cggcgtgcca gtccgcttcg atgcgcccgg ccgagacctc ccggtaagtg tgttcatcga
    10081 ttgcctcgat gcgccgcacg tcgatggcgc cgccgtagcg gcggctgcag tcctgcgccg
    10141 ggcggtacca tgcgccgcgg tgctcgaata tgcgtcccgc cggtcgcgcg cagcgcacat
    10201 ccgagacaat gggattgcag ggatgcgcga tccagttcct gctgaggagg gaatccgagt
    10261 gatacaggaa tacctcatcc cagctagatg cctcgcggtt ctcgcacacg gtggcgaaca
    10321 gccagtacct gccgttatgt cgccagatgg tcgagtcata cgccgtgacc cgttccagca
    10381 gattcatgac gaactcccat tcgtcaggaa aactgcggca acggtaaatc gagatggctt
    10441 cgttctcggc cgactccgga atcatgtaca gcgtcccgcc gtcttcaaat acaaacgggt
    10501 acgacaaatg gtatggccgc ttcatgatgg gccgcggcgg caacaggggc gcgccatccc
    10561 ggttgaaccc gatcacggat agataaccgg tgctgtcggc gtactcgcat tcttctatga
    10621 agaccgcata ctgcccttga tactggacga cgaacggatc cgcccagaat cgatccttcg
    10681 gcgggacgag cggggtgaac tccggtcccg ccttcgcagg ctgctcgacg tcggtgatgc
    10741 ggtacatcag catccactgg tcgaagaaca ccagattccg cacgcgtgcg gccagctttt
    10801 cggccacgcg cgtaccgagt cctcgcagca ccgcaagatt gccgggaatc ggcggcaaca
    10861 cctggccgcg atctacgggt tccggacgtg ccgggcgata cctgtgcagg aacgcatcag
    10921 gaccaagccg gccaagctcg cgcagtcggc gcggggcgta gctcgcacat ttccagagca
    10981 tgcgatccag cgtccgggcg ggagaaatcg tatccgtggg cgaccaggat cgctccagaa
    11041 ctgccacgtc ggctccgccg cctgcaacca tcagcagatc agtggccgtc gttggctgcc
    11101 tcagcaggac ttcccacacg gccggcaacg ggcattgaaa ggtcgcatct tcgccgcagc
    11161 cgtagtacca gacaccgcag cgcgccgcat cgcagatttt tccgcgcagc gcacggaagc
    11221 tgaaccgaag cagtacatcc agccggtagt ccgcaaccgc tggcgcgtcc gccggaacaa
    11281 tcacatcaaa cccgccgtca gtttcggcac gaacatcgat cgctggaacg ccatgcagga
    11341 gtgccgccgc atcgactgct tgcaatgcac cccgtcggcc tccgaaccgc ttgcgatcca
    11401 ggtgcagcca ggcacgtgcc aacagactcc gccaaccggt ttggcagacg cggccggcgg
    11461 tcgcgtcgtc cggggcaacg ccgcaagcgc gcacgacaag cgcaagctcg acgagtccat
    11521 cgcgcctgat ggcctccagc gctcttgcct gccaggccgg caaagtcgtt ccgtcaacca
    11581 tcactccaag tcttagtcga tgcatccctg gttccgtgtg cgtcggtaca ccttgctcac
    11641 ctttccggcg aagtgcgatc ggagaccagc gcaaaggtga tcagccagta cagcatgtcg
    11701 aacaccgtgt gcgcgaacat ggacaagaat acgaacagga acaccagcgc gatctgaagg
    11761 ctgcgatccg cgaagtattt tccggtccat agcatgcgcg ccattgccag ccagcatgcg
    11821 ataccgacga cgccgtactc ggcggcgagc aggatgaact ggttatgcgt tccgacggcg
    11881 agcaaccagc tggacgtggc gccggcaccg gcgccctcga tcggggactg caggaacagt
    11941 tccagtcctg ccctgagggc ttcatatcgt tcctgcgagc tttcatcgtt gaagctcggg
    12001 ttggaaaaga agctgatgcg gtcctggaga ttccccatcg cgccgtccag atcggcgcgg
    12061 cttccgaggt atccctgaag ggcggccaac acaaacggaa tcgctgcaca aaaggcaaga
    12121 acgtatgcat agccggcggc aggaaggacg cggcgatact gcaggaatgc ccacagcacc
    12181 atccatgcaa cgatcgccga gcgcgagaat gtcatcgcgg ccccgagccc ggtgagaaga
    12241 aagagcggca tcctgtatcg catgctcacc gaaggacagg cgaacaggaa tgtgatcagg
    12301 attacctcgc cggcaatcgt cggattgatg aacgtggcgg aagcgcggcc gggaaccgta
    12361 ccgggcgttc cgggagggta gaacagtccc ggcaccagaa aatccagaat cacgacgaca
    12421 ggagcaagca ctgcgacggc acggaaaatg ccgtcatatc cgaaatcacg ccagctccac
    12481 agcgcaaagc ccagcgccac ggccagcaca acgaactgga tccttgtcag gatcgccgcg
    12541 tcgcgcgcaa cttccccgcc tgatacgccg cccagcaggt gaatcaggtt gatgagaacg
    12601 taggcgagag cccacagcgc gaacggcgaa aacaggtaga aaagcaaggc ccttgtctgg
    12661 aaaaaaagcg gggcaagcag cagtacgaat gcgaaatacc aaagcttcgg tggaaggctc
    12721 gcgttcagcg cgatcccgta gttcggaaga ttcagaaaca gcaccaggag aaatagcgaa
    12781 agcaccacgc cgatgtacgt ccggtagcca ttccagagca acgcagtcgt cgcacgcggg
    12841 accgcgcggc tgccgtagta cagcacgggc ctggcccttc ttgcttcgct gtcttgcatg
    12901 ttagctcccc gcactggatc tttcattcgc aggaatgttc aggctggcag cgacggttcg
    12961 ccaggctgat cgacataacc ggtgggggcc gtcgccccgg actctgttcg atgtgcgcat
    13021 ttgcatgggt atgaagcggg tctgatccaa tcgcctagct tgcgcactgg ccgaacaatt
    13081 gctcccacat cgacgaaatc cgttcgaccg aaaagcgctc gcgcgtgcac agtgcggcac
    13141 gcgcaaacgc ttgcctctca ctcgttcccg tcatcaggct gccgagtgcc gcttcgagtg
    13201 cggcaaggtc gcctggccgg atcagcaagc catcgatccc cggtcgtatg atgtcgcgcg
    13261 gcccggtatc gcaatcgaag ctgacggcag gcaggccata gctcatcgct tcaaccagtg
    13321 tgttcgggaa accctcgaat cgtgaggtca tcacatacag gtctgcccgc tcgtaccacc
    13381 tgcccatgtt tcccacgcgc cccggcagga atacgcgcct gcgcaagccc gcaatgccga
    13441 ccttcccttc gagcgcagcg cgctccggcc cctcgccaag tatcacgaga tcccagtcgg
    13501 ggtgacgggg cgcaaggcgc gtgaacacgt cgatcagcag atcaaaattc ttctcctcgc
    13561 tgagtcgccc
//
LOCUS       DATLGF010000353         5490 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_151735, whole genome shotgun sequence.
ACCESSION   DATLGF010000353 DATLGF010000000
VERSION     DATLGF010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5490
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151735"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VM406_10865"
     CDS             <1..358
                     /locus_tag="VM406_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HVL76505.1"
                     /translation="AQPDLLGLLRARPEQAEQVRLRVGDLEMNLIAREVQREGKVIDL
                     QNKEFQLLEFLMRRVDQVVTRTMLLEGVWDFHFNPNTNLVDAQMSKLRAKIDKPFAQP
                     LVHTIKGVGFKISTRP"
     gene            355..1728
                     /locus_tag="VM406_10870"
     CDS             355..1728
                     /locus_tag="VM406_10870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVL76506.1"
                     /translation="MIRSLRLFAPRSFSFRVGIIIFLALCVILATARVVFYFQSIEAA
                     RASVRRLLDAHVEEIANGYERNGAAYARSLVEGIVEEGRDKNLYLEFQQGESFSGNLA
                     VRPYVPLRSRGVFEVEVPREDDDAQHLMFRVYEYPNNVVIRAAYDMESVDLLRRTLLA
                     SLAANAVIAFALSLLLSFLIVKLFDRYFRNFNVACNEIMSGNLDYRIATTGGKDEFEK
                     LAANLNRMLDWLKGLLGMIKDSNNAIAHDIRTPLSRLRLEMSALAQEPGLSGALRAKV
                     EKQIEQVDGLVDMFDSLLVIAKAESRSGVELFEAFDLAQLVREVSAFYAPILEEKGLP
                     LTLDLPGYALAFTGDKQLISQALVNLLDNASKYSRDGGEIVVSLFDGQDAVRLTVADR
                     GAGIPPALMHKARERFVRGDASRHSKGYGLGLSLVNAVATLHHGELRLEDNLPGLRAT
                     LLLHRLG"
     gene            complement(1730..2302)
                     /locus_tag="VM406_10875"
     CDS             complement(1730..2302)
                     /locus_tag="VM406_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVL76507.1"
                     /translation="MRYPKEHKEETRRRIVDKAGERFRNEGIEAVGLANLMQSVGLTV
                     GGFYAHFGSREDLVAEACEAGFARTTAALRSYLDTQPRGERTAAFIDAYLSPQHRDEP
                     GAGCMIAANGAELARHPASTRAAFTAQVDAWIGLIDAVLAEDGLKADARALAAGLVGT
                     MVLARAVNDPARSDAFLESGRKSLKANLRR"
     gene            complement(2310..3059)
                     /locus_tag="VM406_10880"
     CDS             complement(2310..3059)
                     /locus_tag="VM406_10880"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVL76508.1"
                     /translation="MTSPDPVDMAALAFLQPQQGSPNPARVESPDEWVETAEGRIAVW
                     RRGAGKRVLLVHGWAGSHGDLHAFVAPLAAAGCEVIAIDLPAHGMSDGRIASIPDLAR
                     ALLHVAGRYGPLCGVVAHSVGCAAVGVALRDGLPAPRTVLIAPPARYADFASDFARMV
                     GIDPQALLDALRRRDIDVDGIDFPAMAPRLASRALIIHSRDDRVVPYANGEALAAAWP
                     GSGFLSCEGLGHGRILADAAVIEHAVRFLTD"
     gene            3241..4071
                     /locus_tag="VM406_10885"
     CDS             3241..4071
                     /locus_tag="VM406_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HVL76509.1"
                     /translation="MRHQSPAVTGELGYVVPSTEKPYNYMYEPPPGQAWENCEYERRP
                     MRIHDARMLASALGLEQSGFELRDHPLSGIDFDDGDAVRAGYYREIELLALALTGGEK
                     AIVFDHQVRRREPGRPALAFGRKGDGKRPSAVGRVHNDYSEQSGRRRLQLVLGDAPND
                     RPYMILNFWRPFIDPAIDTPLAVCDARSFARGDWVAADIRYPDRTGEIYLARYSPMHM
                     WYYYPRMTPDEVLVFKSYDSRLDCPVRMTPHCAFDDPSAPADAPLRKSIEARCLVILE
                     "
     gene            4080..4700
                     /locus_tag="VM406_10890"
     CDS             4080..4700
                     /locus_tag="VM406_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HVL76510.1"
                     /translation="MQVIEKPQLEFWFEFGSNYSYLSVMRIEAAAREHQVDIVWKPFL
                     LGPIFKELGWESAPFVQQKEKGAYVWRDMERQAAKYGLPFRRPSTFPRVAVLPMRVAV
                     HGAGEPWIGAFCREVMLQNFFHDIDVNREDNVRRALERAHADPDAVLAAATGEENKLR
                     LRRQTEEARRRGIFGAPTFFAGDEMFWGNDRLEDALEWAAGRRAHA"
     gene            4755..5249
                     /locus_tag="VM406_10895"
     CDS             4755..5249
                     /locus_tag="VM406_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVL76511.1"
                     /translation="MSASPAPASGRVALPVEDAPPRSKPSLYPTIFAERMAGRVKHPL
                     GDLFGLSNFGVNLTRLAPGSVSALNHAHSRQDEFIYVIEGNPTLVTGGEEVQLSPGMC
                     AGFKAGTGIGHHLENRSGADVVYLEVGDRSVGDVVNYPRDDLQAVMGEDGKWRFTRKD
                     GTPY"
     gene            complement(5284..>5490)
                     /locus_tag="VM406_10900"
     CDS             complement(5284..>5490)
                     /locus_tag="VM406_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76512.1"
                     /translation="KAMLGPWWRWKIAALFVFGGIALLFVAAIAGVLVVTAIAFALLA
                     IVLLRVRSWWRGLRRSNSSQVRRR"
BASE COUNT          950 a         1786 c         1861 g          893 t
ORIGIN      
        1 cgcgcagcct gacctgctcg gcctgctccg ggcgcgtccg gagcaggccg agcaggtcag
       61 gctgcgcgtc ggcgacctgg aaatgaacct gatcgcacgg gaggtgcagc gcgaaggcaa
      121 ggtcatcgac ctgcagaaca aggagttcca gttgctggag ttcctgatgc gcagggtcga
      181 ccaggttgtc acgcgtacca tgctgctcga gggcgtctgg gattttcatt tcaatccgaa
      241 cacgaacctg gtcgatgcgc agatgagcaa gctgcgcgcc aagatcgaca agccgtttgc
      301 acagccgctg gtgcacacga tcaagggcgt cggattcaag atcagcacac gcccatgatc
      361 cgcagcttgc gcctgttcgc gccgcgcagc ttctcgttcc gcgtcggcat catcattttc
      421 ctggcactgt gcgtgatcct cgccaccgcg cgcgtggtgt tctacttcca gtcgatcgag
      481 gccgcacgcg ccagcgtgag gaggctgctc gacgcccacg tggaagagat cgccaacggt
      541 tatgagcgca atggcgccgc ctacgcgcgg tcgctggtcg aaggcatcgt ggaggagggg
      601 cgcgacaaga acctgtatct ggaattccag cagggagaat cgttctccgg caacctggcg
      661 gtccggccgt atgtgccgct gcgctcgcgc ggcgtgttcg aggtcgaggt gccgcgcgag
      721 gacgacgatg cgcagcacct gatgttccgg gtgtatgagt atccgaacaa cgtcgtcatc
      781 cgcgccgcct acgacatgga gtcggtcgac ctgctgcgcc gtacgctgct ggcctcgctg
      841 gcggccaatg cggtcatcgc gttcgccctg tccttgctgc tgagcttctt gatcgtcaaa
      901 ctgttcgatc gctatttccg caacttcaat gtggcctgca atgaaatcat gtcaggcaac
      961 ctcgactacc ggatcgcgac caccggcggc aaggacgaat tcgagaagct tgcggccaac
     1021 ctcaaccgca tgctggactg gctcaagggc ctgctcggca tgatcaagga ctccaacaac
     1081 gcgattgccc acgacatccg cacgccgttg agccggctgc ggctggaaat gagcgcgctg
     1141 gcgcaggagc ccggcctgag cggcgcgctg cgcgcaaagg tcgaaaagca gatcgaacag
     1201 gtcgacggcc tggtcgacat gttcgacagc ctgctcgtga tcgccaaggc ggagtcgcgc
     1261 tcgggagtgg agttgttcga agcgttcgac ctggcccagc tggtgcgcga ggtgtccgca
     1321 ttctatgccc cgatcctgga ggagaagggc ctgccgctca cactcgacct gccgggctac
     1381 gcgctggcct tcaccggcga caagcagttg atcagccagg cgctggtgaa cctgctggac
     1441 aacgccagca agtactcccg cgatggcggc gagatcgtcg tatccctgtt cgacgggcag
     1501 gacgcggtgc gcctcacggt cgccgaccgc ggcgccggca tcccgccggc cctgatgcac
     1561 aaggcaaggg agcgcttcgt gcgcggcgac gcgagccggc acagcaaggg ctacggcctg
     1621 ggactgagcc tggtgaacgc ggttgcgacg ctgcaccatg gcgagctgcg gctggaagac
     1681 aacctgccgg ggctgcgggc gacgctgctg ctgcaccggc tggggtgagc taccgtcgca
     1741 ggttcgcctt caagctcttg cgtccggatt cgaggaaggc gtccgagcgt gccggatcgt
     1801 tcaccgcgcg cgccagcacc atggttccga ccagtcccgc cgccagcgct cgcgcgtccg
     1861 ccttcagccc atcctctgca agtaccgcat cgatcaggcc gatccacgca tcgacctgcg
     1921 ccgtgaaggc ggcgcgggtg gacgccgggt ggcgcgcgag ttccgcgccg ttggcggcga
     1981 tcatgcagcc ggcgcccggt tcgtcgcggt gctgcggcga caggtaggcg tcgatgaagg
     2041 cggccgtgcg ctcgccgcgc ggctgcgtgt ccaggtagga acgcaatgcc gccgtggtcc
     2101 tggcgaaccc ggcttcgcag gcttcggcca cgaggtcctc gcgcgaaccg aaatgcgcgt
     2161 agaagccgcc gaccgtcagg ccgacgctct gcatcaggtt ggccaggccg accgcttcga
     2221 tgccctcgtt gcgaaagcgc tcgcccgcct tgtcgacgat ccggcgccgg gtttcctcct
     2281 tgtgttcctt cgggtagcgc atgtcggtct caatctgtca gaaagcggac cgcgtgttcg
     2341 atgacggccg cgtccgcaag gatacggcca tggccgaggc cttcgcaaga caggaacccg
     2401 gaccccggcc aggccgctgc cagcgcttcg ccattcgcgt acgggacgac gcggtcgtcg
     2461 cgcgaatgga tgatcagcgc ccgcgacgcc agtcgcggcg ccatagccgg gaaatcgatg
     2521 ccgtccacat cgatgtcgcg ccggcgcagc gcgtccagca aggcctgcgg atcgatcccg
     2581 accatgcgcg cgaagtcgct ggcgaaatcc gcatagcgcg caggaggggc gatcagtacc
     2641 gtgcgtggcg ccggcagacc gtcgcgcagc gcgacgccga cggcggcgca gccgaccgaa
     2701 tgggcaacga cgccgcacag cgggccgtag cggccggcca cgtgcaggag cgcgcgggcg
     2761 agatcgggga tcgatgcgat cctgccgtca gacatgccgt gcgccggcag atcgatcgca
     2821 atcacctcgc atcctgcggc tgccagcggt gcgacgaagg cgtgcaggtc gccatgcgag
     2881 cctgcccatc catgcacgag caggacccgt ttgccggcac cgcgccgcca gacggcgatg
     2941 cggccctccg cagtctcgac ccactcgtcg ggggactcaa cgcgcgccgg gttcggggat
     3001 ccttgctgag gctgaagaaa ggcgagggct gccatgtcga cggggtcggg ggaggtcatg
     3061 cggcactcct tttgaaagat gctcgtaata ttacaagcgt aatcatcgat gaggcaagcg
     3121 cctcggtgga tggcgcgaca tgcgggtcga tcatggctgc catgccgttt ttgcgtttga
     3181 gtacgggggc acggctcgct acagtcgcag catcaccatg caggagcgac cgatatggac
     3241 atgcggcatc aatccccggc ggtaaccggc gaactcggct atgtcgtgcc ttccacggag
     3301 aaaccctaca actacatgta cgagccgccg cccggacagg cgtgggaaaa ctgcgaatac
     3361 gaacgccggc caatgcgcat ccatgacgcg cggatgctgg cttccgcgct cgggcttgaa
     3421 cagagcggtt tcgagctgcg cgatcatccg ctttccggca tcgatttcga cgacggcgac
     3481 gcggtacgcg ctggctacta ccgggaaatc gagctgctcg cgcttgcgct gaccggcggc
     3541 gaaaaggcga tcgtgttcga ccaccaggtg cgcaggcgcg aaccggggcg cccggcgctg
     3601 gccttcggcc gcaagggcga cggcaagcgg ccgagcgcgg tggggcgtgt ccacaacgac
     3661 tacagcgagc agtccggccg gcgccgcctg cagctggtgc tgggcgacgc gccgaacgat
     3721 cgtccgtaca tgatcctcaa cttctggcgc cccttcatcg atccggccat cgacacgccg
     3781 ctggccgtct gcgacgcgcg cagcttcgcg cgaggcgact gggtggcggc ggacatccgc
     3841 tatccagacc gtaccgggga aatctacctt gcgcgctact cgccgatgca catgtggtac
     3901 tactatccgc gcatgacacc ggacgaggtg ctggtattca agtcctatga ttcccggctc
     3961 gactgcccgg tacgcatgac gccgcactgc gcgttcgacg atccgagcgc gccggccgat
     4021 gcgccgctgc gcaaaagcat cgaggcgcgc tgcctcgtca ttcttgagtg aggattgcca
     4081 tgcaggttat cgagaagccg caactggagt tctggttcga gttcggcagc aactacagct
     4141 acctctcggt catgcgcatc gaagcggccg cgcgcgagca tcaggtggat atcgtgtgga
     4201 aacccttcct gctggggccg attttcaagg agctcggctg ggaaagcgcg cccttcgtgc
     4261 agcagaagga aaagggcgcc tacgtgtggc gcgacatgga gcggcaggcg gcaaagtacg
     4321 ggctgccgtt ccggcgtccg tccacgtttc cgcgcgtggc ggtgctgccg atgcgggtgg
     4381 ccgtccatgg cgccggcgag ccgtggatcg gcgccttctg ccgcgaagtg atgctgcaga
     4441 atttcttcca cgacatcgac gtcaaccgcg aggacaacgt gcggcgggcg ctggagcgcg
     4501 cgcacgcgga tccggatgcg gtgctcgcgg ccgccaccgg cgaggagaac aagctgcgcc
     4561 tgcggcgtca gaccgaggaa gcgcgcaggc gcggcatctt cggcgcgccg accttcttcg
     4621 ccggcgacga gatgttctgg gggaatgacc gcctggagga tgcgctggaa tgggcggcgg
     4681 gcaggcgggc ccacgcctga cgcggcggtg cggcatcata tcggctgtgt ccgttgttac
     4741 cgaaggagtc gcccatgtcc gcatcacccg cgcccgcctc cggccgcgtt gcccttccag
     4801 tcgaggatgc gccgccacgc agcaagcctt ccttgtatcc gaccatcttc gccgagcgca
     4861 tggccggccg cgtcaagcat ccgctcggcg atctgttcgg actgagcaat ttcggcgtga
     4921 acctcacgcg cctggccccg ggctcggtgt ccgcgctgaa ccatgcgcat tcacgccagg
     4981 atgaattcat ctatgtgatc gaaggcaatc cgacgctggt gacgggcggc gaagaggtgc
     5041 agcttagtcc aggcatgtgc gccggcttca aggcgggaac gggaatcggc catcacctcg
     5101 agaaccgcag cggcgcggac gtggtgtacc tggaggtcgg cgaccgctcg gtcggcgatg
     5161 tggtgaacta tccgcgcgac gacctgcagg cggtgatggg cgaggatggc aagtggcgct
     5221 tcacgcgcaa ggacgggacc ccgtactgag gtccgctccc gcgccggcct tgccggggcg
     5281 gatctatctg cgccggacct gcgacgaatt cgagcggcgc aggccgcgcc accagctgcg
     5341 cacgcgcagc agcacgatgg ccagcagcgc gaaggcgatt gccgtgacga cgagcacgcc
     5401 ggcgatagcg gcaacgaaca gcagcgcgat gccgccgaac acgaacagcg ccgcgatctt
     5461 ccagcgccac cacggaccca gcatcgcctt
//
LOCUS       DATLGF010000354         7611 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_171549, whole genome shotgun sequence.
ACCESSION   DATLGF010000354 DATLGF010000000
VERSION     DATLGF010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171549"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VM406_10905"
     CDS             complement(<1..455)
                     /locus_tag="VM406_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HVL76513.1"
                     /translation="MNAAARTSEAFRFGFWPRVLVFVLLAYIIYASSQLGFSWTRFVS
                     GLDNGARFLSRMFPPDMTKMDILWKGISESLQIAVLASVGGILLSLPIGLMGARNLMP
                     GWLSWSARALVAVCRSLHPVIVAILFVKAVGFGALAGILALIVASIGFIG"
     gene            complement(452..1219)
                     /gene="phnC"
                     /locus_tag="VM406_10910"
     CDS             complement(452..1219)
                     /gene="phnC"
                     /locus_tag="VM406_10910"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HVL76514.1"
                     /translation="MSHALEIRGLVKAYRAGNPILKGIDLSIDGAGLTAIIGPSGTGK
                     STLIRCINRLVDPSAGSILLRSAEGSIDLAALKGRSLRENRRRIGMVFQEYNLVERLT
                     VMENLLCGRLGYVGAWNAWRRKFPQEDIDHAFDLLDTVGLAAFANQRADALSGGQRQR
                     VGIARALMQRPQVLLADEPTSSLDPKTSFEIMWLLKEQGRINNIPVIVNIHDVELARR
                     YADRIIGMSEGHVVYDGAPEGLTDDYLKRIYGGEDWL"
     gene            complement(1383..2369)
                     /gene="phnD"
                     /locus_tag="VM406_10915"
     CDS             complement(1383..2369)
                     /gene="phnD"
                     /locus_tag="VM406_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HVL76515.1"
                     /translation="MNRFKLLAGIALAIPAFAYAQDNCPHRGDLDTPYCDANRDMVAD
                     TPTDPKRLKNPSTLVFTYTPVEDPAVYEKAFAPFTQHLSQCMERRVVFFQVQSNAAEI
                     EAMRSGRLHVGGFSTGPTAFAVNIAGAVPFAVKGDEKGYQGYHLYVIVKKDSPIQTMA
                     DLKGKKVAHTSPSSNSGHMAPLALFPKLGVVPDKDYKITFSGKHDQSVMGVNSGDYDA
                     AAVASDVFERMAHRGQVKLEDFRIIYRSEKFPTSSFAYAHDLEPAFRDRMLKCFYDYR
                     FTDEMKKTFDGADRFFPITYKKDFEIVRNVAESAGEKFNRAGYEREAAKAKK"
     gene            2564..3244
                     /locus_tag="VM406_10920"
     CDS             2564..3244
                     /locus_tag="VM406_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL76516.1"
                     /translation="MKILLVEDDQDMSKALSRALERRGFAVTACFDGASGLRQMRDTD
                     PDLVILDLNIPVIDGLHLLQRAREQELDTPVIVLTARGAVGDRIAGLNAGADDYLAKP
                     FDLNELEARIHALLRRKKGGSEAVIKCGDLHYERSSGAFYHHGRPLEFTPREHALLKA
                     LIAKPGHAVAKERLFRLVFALEESAQIEAIEVIIHRLRKKLAPTRTEIMTLRGLGYLL
                     RAAAEQQA"
     gene            3241..4629
                     /locus_tag="VM406_10925"
     CDS             3241..4629
                     /locus_tag="VM406_10925"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HVL76517.1"
                     /translation="MKPGARSVRAYLLGWIIAPIALFIVIDTFTLYRNALESVNTAYD
                     RMLIATAHSIGDLLRYENGELKVVLPHATLEIYEADRLSRMIYRVEDFGGQFLSGDAA
                     LPRYTGARSNEAAYPTVVRIYEDQYRGELVRVAALFQPVMSNDASGGAVVQVAETIDN
                     RRSLAQKILRDTLLRQAVLLLVVAVVTLIVVNHALRPLSVLREQLDQRRADDLSPLLA
                     PSAPKELQPVTNALNSLMGRLQRLLDYQHRFVANASHQLRTPLAVLKTQLQSGLRGDA
                     PAEVVLQEMRGTVERATMLANQLLSLAKVEQLRGRGMRQLCDLTMIARSTALDLSPLI
                     AEKNLDFALDAAPAQVRGHPWMIGELISNLLHNAIRHTPAGAQLGVTVSVESDHVRLC
                     VWDSGAGLPASHDRERFEPFAHAHGSKGGGLGLAICKEIADSMNARITLADRCVDGRK
                     AGAEAVVEFPKG"
     gene            complement(4865..5155)
                     /locus_tag="VM406_10930"
     CDS             complement(4865..5155)
                     /locus_tag="VM406_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HVL76518.1"
                     /translation="MNKSDIIDTVAADHELTKVKARQIIDQVFGFIGTDLKKTGRFAY
                     PSLGTFTVTQRAARKGRNPKTGEPLKIKASKGVRFRPAPSLKSMVEKVKIAK"
     gene            complement(5310..6116)
                     /gene="hpaI"
                     /locus_tag="VM406_10935"
     CDS             complement(5310..6116)
                     /gene="hpaI"
                     /locus_tag="VM406_10935"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HVL76519.1"
                     /translation="MSTNAFKSALAACMPQIGLWLCLANADAAEVCATAGFDWLLIDG
                     EHAPNDVRTILAQLRAVAPYRAHPVVRPVSGDVQLIKQLLDVGAQTLLVPMVETAGQA
                     RELVLATRYPPQGIRGDGSPIVRASRWGLEADYLRTANDEVCLLVQVETRRGLDNLDA
                     ICAVDGVDGVFIGPADLSASLGHRGNPGHPDVQAAIDAAIATICAHGKAAGILTSDTG
                     HARRYLGLGCTFVAVGVDVRLLALATRALAAEFAQRENGPAPYQKDGLGY"
     gene            6244..7029
                     /locus_tag="VM406_10940"
     CDS             6244..7029
                     /locus_tag="VM406_10940"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HVL76520.1"
                     /translation="MSRSVHHRSRRCFIAGAAVFLLGAWLPIAAPAADKAGARPWIIA
                     ADASFPPYAYLDPANGRPSGLDTEIVSAALDAVGQPHEIRLFPWERAKKMLESGQVDA
                     AYQFVGTPERRSQYRLAGPIRNGVTVFMARKDGPADYRTLADLQPYLLGTVHGFAYTE
                     EFDRAPLRKDGGAVNVEQLLRKLLAGRVDVIVGDRSQLLFLARRMGVAGEVKIIGRPL
                     AEVPRFVGFPKGGEQQAVRFEKGLEIIRQNGTLNRITARWERQ"
     gene            complement(7055..>7611)
                     /locus_tag="VM406_10945"
     CDS             complement(7055..>7611)
                     /locus_tag="VM406_10945"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL76521.1"
                     /translation="PDTMTRAVRQALDRFGALHLAVNNAGVTGPQQTLIEDLSLQDWE
                     NVVGTDLTGMFLSLKAELPAIVASGGGAIVNLSSANGIVGVPGLAAYTCAKHGVVGLT
                     KSVALEYADKGVRVNCIGPGYVATPRMLQMPRTALDELAALHPLGRLAHRTEVAELTA
                     FLLSARAGFCTGGFYPVDGGYTAR"
BASE COUNT         1296 a         2433 c         2562 g         1320 t
ORIGIN      
        1 ccaatgaagc cgatcgaggc cacgatcagc gcgaggatgc cggccagcgc gccgaatccc
       61 accgccttca cgaacaggat cgcgacgatg accggatgca gcgaacggca gaccgcgacc
      121 agcgcacgcg cgctccatga cagccagccc ggcatcaggt tacgcgcgcc catcaggccg
      181 atcggcaggc tcagcaggat gccgcccacc gaagccagca ccgcgatctg caggctctcg
      241 gagatgccct tccagaggat gtccatcttc gtcatgtccg gcgggaacat gcggctcagg
      301 aagcgcgcgc cgttgtcgag cccggaaacg aagcgcgtcc aggaaaaccc gagctgcgac
      361 gacgcataga tgatgtacgc gagcaggacg aagacgagca cccggggcca gaagccgaac
      421 cggaatgcct cggatgtgcg tgccgccgcg ttcacagcca gtcctcgccg ccgtagatgc
      481 gcttgagata gtcgtcggtc aagccttccg gcgcgccgtc gtagacgaca tggccttcgg
      541 acatgccgat gatgcggtcc gcatagcggc gcgcgagctc gacgtcatgg atattgacga
      601 tgaccgggat attgttgatc cggccctgtt ccttcaacag ccacatgatt tcgaacgagg
      661 tcttcgggtc gagcgaggag gtcggctcgt cggcaagcag cacctgcgga cgctgcatca
      721 gcgcccgggc gatgccgacg cgctggcgct gcccgccgga caacgcgtcg gcgcgctggt
      781 tggcgaacgc ggccaagccg accgtgtcga gcaggtcgaa cgcatggtcg atgtcttcct
      841 gcggaaactt gcggcgccac gcattccatg cgcccacata gccgagccgg ccgcacagca
      901 ggttttccat tacggtcagg cgttcgacca ggttgtattc ctggaacacc atgccgatgc
      961 gacgccggtt ctcgcgcagc gagcgtccct tgagcgcggc caggtcgatg ctgccctcgg
     1021 ccgagcgcag caggatcgat cccgcgctcg gatcgaccag gcgattgatg cagcggatca
     1081 gcgtggactt gccggtgccg gaagggccga tgatggccgt caggccggcg ccgtcgatgg
     1141 acaggtcgat ccccttgagg atcgggttgc cggccctgta tgccttgacc aggccgcgga
     1201 tttcgagtgc gtgggacatg agtcgtcagt gttttgtgag gcggcggacg tcgagcatcc
     1261 cgtggtcggc cgcgatgcgc ggcgcaccag cgggtacgcc tgcatctgcc ggggcgggcc
     1321 gaaggatggg ggaagagccc gccccctttc atccgctccg aagggcggac gggcagccgg
     1381 tgctacttct tcgccttggc cgcttcccgc tcgtagccgg cgcggttgaa cttctcgcct
     1441 gccgattcgg ccacgttgcg gacgatttcg aaatccttct tgtacgtgat cgggaagaaa
     1501 cgatccgcgc cgtcgaaggt tttcttcatc tcgtcggtga agcggtagtc gtagaagcac
     1561 ttgagcatgc ggtcgcggaa ggccggttcc aggtcgtgcg cgtaggcgaa tgaagaggtc
     1621 gggaacttct cgctgcggta gatgatgcgg aagtcctcca gcttcacctg gccgcgatgg
     1681 gccatgcgct cgaacacgtc ggatgcgacg gcggctgcgt cgtagtcgcc ggaattgaca
     1741 cccatcaccg actggtcatg cttgccggag aacgtgatct tgtagtcctt gtcgggtacc
     1801 acgcccagtt tcgggaacag ggccagcggc gccatgtggc ccgagttgga agacggcgag
     1861 gtgtgggcaa ccttcttgcc cttgaggtcg gccatggtct ggatcgggct gtccttcttg
     1921 acgatcacat acaggtggta gccctggtag cccttctcgt cgcccttgac ggcgaacggc
     1981 acggcgccgg cgatgttgac tgcaaacgca gtcgggccgg tcgagaagcc gcccacatgc
     2041 aggcggcccg agcgcatcgc ctcgatttcc gcagcattcg actggacctg gaagaacacc
     2101 acgcggcgct ccatgcactg ggaaagatgc tgcgtgaacg gggcgaacgc cttttcgtac
     2161 acggccggat cttccaccgg cgtatacgtg aacaccagcg tgctcggatt cttcaggcgc
     2221 ttggggtcgg tgggcgtgtc ggccaccatg tcgcggttgg cgtcgcagta cggggtatcg
     2281 aggtcgccgc gatgcgggca gttgtcctgc gcgtaagcga aagccgggat ggccagtgcg
     2341 attccggcca gcagtttgaa gcggttcatg ttgtctcctt cattgttttt ttggcctgca
     2401 agcaagagtg taggaagatt gggcgctacg cgcgagcgcc gagcggcggt ttgcttgcat
     2461 ggcgctttca tgaagctttc aatccgcttt cagtgcaggc ggtttggcat gccggccgga
     2521 atgcgggtat aaatcggcga ttgcctgtgc tgccggagcc cctgtgaaga tcctgctggt
     2581 cgaagacgat caagacatgt ccaaggcctt gtcgcgcgcg ctcgagcggc gcggcttcgc
     2641 ggtcaccgcc tgcttcgacg gcgcgagcgg cctgcgccaa atgcgcgaca ccgatcccga
     2701 cctggtcatc ctcgacctga acattcccgt catcgacggc ctgcacctgc tgcagcgcgc
     2761 ccgcgagcag gagctcgata cgccggtcat cgtgctgacc gcgcgcggcg ctgtcggcga
     2821 ccgcatcgcc ggcctgaacg ccggggcgga cgactacctt gccaagccct tcgacctcaa
     2881 cgagctggag gcgcgcatcc atgctctgct gcgccgcaag aagggcggca gcgaggcggt
     2941 gatcaaatgc ggtgacctgc actacgagcg cagctcgggc gcgttctacc accacggcag
     3001 gccgctcgaa ttcacgccgc gcgagcatgc gctgctcaag gcgctgatcg ccaagccggg
     3061 gcatgcggtg gcgaaggagc ggctgttccg gctggtgttt gcgctcgagg aaagcgcgca
     3121 gatcgaagcg atcgaggtca tcatccatcg cctgcgcaag aagcttgcgc ccacgcgcac
     3181 ggagatcatg accttgcgcg gactcggata cctgctgcgc gcggccgcgg agcagcaggc
     3241 atgaagccgg gcgcgcgctc ggtgcgcgcc tatctgctcg gctggatcat cgcgccgatc
     3301 gcgctgttca tcgtgatcga cacattcacg ctgtatcgca acgcgctgga gtcggtgaac
     3361 accgcctacg accgcatgct gatcgccacc gcacattcga tcggcgatct gctgcgctac
     3421 gagaacggcg agttgaaggt ggtgctgccg cacgcgacgc tggagatcta cgaggccgac
     3481 cgcctctcgc gcatgatcta ccgggtcgag gatttcggcg ggcagtttct ctccggcgac
     3541 gcggccctgc cgcgatacac cggcgcgcgc agcaacgaag ccgcctatcc gaccgtggtg
     3601 cggatctacg aagaccaata ccgcggcgag ctggtgcggg ttgcggcgct gttccagccg
     3661 gtcatgagca atgacgcgtc cggcggcgcg gtggtccagg tggccgaaac cattgacaac
     3721 cgccgcagcc tggcgcagaa gatcctgcgc gacacgctgc tgcgccaggc ggtgctgctg
     3781 ctggtcgtag ccgtcgtgac gctgatcgtg gtcaaccatg cgctgcgccc gctgtcggtt
     3841 ctgcgcgaac agttggatca gcgccgcgcc gacgatctgt ctccgctgct cgcgccttcg
     3901 gcgccgaagg agctgcagcc ggtgacgaat gcgctcaaca gcctgatggg gcggctgcag
     3961 cgcctgctgg actatcagca tcgcttcgtc gccaatgctt cgcaccagct gcgcacgccg
     4021 ctggcggtgc tgaagacgca gctgcagtcc ggtttgcgcg gcgatgcgcc ggccgaagtg
     4081 gtgctgcagg agatgcgcgg cactgtcgaa cgcgccacca tgctggcgaa tcagctgctg
     4141 tcgcttgcca aggtcgagca gctgcgcggg cgtggcatgc ggcagctgtg tgatctcacc
     4201 atgatcgcgc gttccaccgc attggacctt tcgccactga tcgcggaaaa gaacctcgac
     4261 tttgcgctcg atgccgcgcc tgcccaggtg cgcggacatc catggatgat cggcgagctc
     4321 atttccaatc tgctgcacaa cgcgattcgc cacaccccgg ccggcgcgca gctgggcgtg
     4381 acggtgagcg tcgaatcgga ccacgtccgc ctgtgcgtct gggacagcgg cgcgggactg
     4441 cccgccagtc atgacagaga gcgtttcgag ccgtttgcgc atgcgcacgg ctccaagggc
     4501 ggcgggctgg ggctggcgat ctgcaaggaa atcgccgatt cgatgaatgc gcgcatcacg
     4561 ctggcggacc gctgcgtcga cggaaggaag gccggcgcgg aggccgtggt cgaatttccc
     4621 aagggctgat gcctgcctgg ggaaggcgga agcgcgcact gcccggcttt atcctgcaca
     4681 gccccggtgc cggtaggtcg gcactttggg ggggaagaag tgatccccgg cgcaagtctg
     4741 atcggcatcc gggcggcgtg ccgaatcaag ggtgggtccg gcaggcgctg gaacggtgag
     4801 cgttcgcgat gctgtggccg gctgaaaaaa gaaagagcgg gaggaccgct ctttctttca
     4861 tcgcttactt cgcgatcttg accttctcga ccatcgactt caggctcggg gcgggacgga
     4921 agcgcacacc tttggacgcc ttgatcttca gcggctcgcc ggtcttcgga ttgcggccct
     4981 tgcgcgctgc gcgctgggtg accgtgaacg tgcccaggct cgggtaggcg aaacgcccgg
     5041 tcttcttcag gtccgtaccg atgaaaccga acacctgatc gatgatctgg cgtgccttga
     5101 ccttggtcag ttcgtggtcc gccgcgacgg tgtcgatgat gtcgctcttg ttcatgactc
     5161 tccttcttga aaaataaccg gcaccgccgg caggaacagc gccctggata aggacgcccc
     5221 aaaaggggcc tcaattatca cttggaaaag tgtcggtgcc tatatgcaaa gggcgccgat
     5281 accacagaat cccgcacgca ggccgcgcat cagtacccga ggccatcctt ttggtacggg
     5341 gctggcccgt tttcgcgctg cgcgaattcc gccgccagcg cgcgcgtcgc cagcgccagc
     5401 aggcgtacgt ccacgccgac cgcgacgaag gtgcagccca gtcccagata gcgacgcgcg
     5461 tgcccggtat cggaagtgag gatgccagcg gccttgccgt gcgcgcagat ggtggcgatc
     5521 gccgcgtcga ttgctgcctg cacgtcggga tgtcccgggt tgccgcgatg gccgagcgag
     5581 gccgacaggt cggctggccc gatgaacacg ccgtcgacgc catccaccgc gcagatcgca
     5641 tcgaggttgt ccagcccacg tcgcgtctcg acctgtacca gcaggcagac ctcgtcgttg
     5701 gccgtccgca ggtaatcggc ctcgagcccc cagcgcgagg cgcgtacgat ggggctgccg
     5761 tcgccgcgga tgccttgcgg cggatagcgt gtggcgagca cgagctcgcg cgcctgcccg
     5821 gccgtttcca ccataggaac cagcagcgtc tgcgcaccca catcaagcag ctgcttgatc
     5881 agctggacat cgccgctgac cggacgcacc accgggtggg cgcggtacgg ggcgacggcg
     5941 cgcaattgcg caaggatggt gcgcacgtcg ttgggcgcat gctcgccgtc gatcagcagc
     6001 cagtcgaagc cggcggttgc gcagacctcg gccgcgtccg cattggccag gcacagccac
     6061 aggcctatct gcggcatgca ggcggcgagg gcggatttga aggcattcgt tgacatgaaa
     6121 tggttgtggg gcgcgtggat gagggaagcg cacgatacta ctgaacggcc tgtcctgcac
     6181 ttttcgactg gaaaaaagcc gcgctccggt ctaacgtgtc gatatgcagc cttgatccga
     6241 ccgatgtccc gctctgtcca ccaccgttcg cgccgttgtt tcatcgccgg tgccgccgtc
     6301 ttcctgctcg gcgcatggct gcccattgcc gcgcccgccg cagacaaagc gggtgcgcgg
     6361 ccctggatca tcgccgcaga tgcatctttc ccgccttacg cctatcttga tcctgccaac
     6421 gggcgcccca gcggactgga taccgagatc gtcagtgcgg cgctggacgc cgtcggccag
     6481 ccgcatgaaa tcaggctctt cccgtgggaa cgcgcgaaga agatgcttga gagcggacag
     6541 gtcgacgccg cgtaccagtt tgtcggcacg cccgaacgca ggtcgcagta ccgcctcgcg
     6601 ggcccgatcc gcaacggggt gacggtgttc atggcgcgca aagacggccc agccgactac
     6661 cgcacgctgg ccgacctgca gccttatctg ctcggcaccg tgcatggttt cgcgtacacc
     6721 gaggagttcg accgcgcgcc gttgcgcaag gatggcggcg cggtcaacgt cgagcagctg
     6781 ttgagaaagc tgctcgccgg ccgtgtcgat gtgatcgtcg gcgaccgcag ccagctgctc
     6841 ttcctcgccc gccggatggg cgtggcgggg gaggtgaaaa tcatcggcag gccgctcgcc
     6901 gaggtgccgc gtttcgtcgg cttcccgaag ggcggcgagc agcaggccgt gcgcttcgag
     6961 aagggactgg aaatcatccg gcagaacggc acgctgaatc ggatcaccgc gcgctgggag
     7021 aggcagtagg catccgcggc ggcggtgcag gtggctaacg cgcggtatac ccgccatcga
     7081 ccggatagaa gccgccggtg cagaagcccg ccctggccga gagcagaaag gcggtgagct
     7141 cggcaacctc ggtgcgatgc gcgagccggc ccagcggatg cagcgccgcc agttcgtcga
     7201 gggccgtgcg cggcatctgc agcatgcgcg gggtcgccac atagccgggg ccgatacagt
     7261 tcacccgcac gcccttgtcc gcatactcga gcgccacgct cttcgtcagg ccgaccacgc
     7321 catgcttggc gcaggtatag gcggcgaggc cgggcacgcc gacgataccg ttggccgacg
     7381 acaggttcac gatcgcgccg cctccgctgg cgacgatcgc cggcagttcg gccttcaggc
     7441 tgagaaacat gccggtcagg tcggtgccga cgacgttttc ccagtcctgc aaggacaggt
     7501 cctcgatcag cgtttgctgc gggccggtca cgccggcatt gttgacggcc aggtgcagcg
     7561 cgccgaagcg gtccagcgcc tgccgcaccg cgcgtgtcat cgtgtccggc g
//
LOCUS       DATLGF010000355         6084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_196754, whole genome shotgun sequence.
ACCESSION   DATLGF010000355 DATLGF010000000
VERSION     DATLGF010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196754"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VM406_10950"
     CDS             <1..978
                     /locus_tag="VM406_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HVL76522.1"
                     /translation="VYADPAHPEAYSRARQRLKDLRAMLRRAVDVPVTSASVRTETIR
                     DFAKAAYLKAVERAKEYIMAGDLMQVQVGQRLKKPYVDAPLSLYRALRSLNPSPYMYF
                     YNFGDIQIVGASPEILVRNEQVADGSRKVTIRPLAGTRPRGATPESDAQLAKELLADP
                     KEIAEHVMLIDLARNDIGRIAETGSVKVTDKLVIEKYSHVQHIVSNVEGTLRPGMSNL
                     DVLKATFPAGTLSGAPKVRAMEIIDELEPVKRGIYGGACGYLSFGGEMDLAIAIRTGV
                     IKDGMLYAQAAAGVVADSVPEMEWQETENKARAVLRAAEQVQDGLDGGI"
     gene            980..1543
                     /locus_tag="VM406_10955"
     CDS             980..1543
                     /locus_tag="VM406_10955"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HVL76523.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGEDVRTYRNDEITLEQIAELNP
                     DRICISPGPCTPNEAGVSVPVLKEFGGKVPILGVCLGHQSIGAAFGGQVVRARQVMHG
                     KTSVIKHSGVGVFKDLPDPYTVIRYHSLAIARESLPDCLEITAWTEDGEIMGVRHKTL
                     AIEGVQFHPESILSEHGHALLKNFLTA"
     gene            1606..2646
                     /gene="trpD"
                     /locus_tag="VM406_10960"
     CDS             1606..2646
                     /gene="trpD"
                     /locus_tag="VM406_10960"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HVL76524.1"
                     /translation="MPITPQQALLRCIEHREIFHDEMLSLFRKIMGGEMSPVMIAALT
                     MGLRVKKETIGEIAAAAQVMREFATKVPVEDTTKLLDIVGTGGDGAHTFNISTASMFV
                     AAAAGARVAKHGGRSVSSSSGSADVLESLGANINLKPEQVAASIKETGIGFMFAPNHH
                     AAMKHAAPVRKELGVRTIFNILGPLTNPAGAPNILMGVFHPDLVGIQVRVLQRLGAQH
                     AVVVWGRDNMDEVSLGAGTMVGELVDGEIREYDIHPEDFGLQMMASRNLKVSGAEESK
                     LRILEALDNVPGPVRDIVILNAGTALYAAGVASSIGDGLLKARETIASGAARSKLEQF
                     VQVTRQLGALNA"
     gene            2655..3455
                     /gene="trpC"
                     /locus_tag="VM406_10965"
     CDS             2655..3455
                     /gene="trpC"
                     /locus_tag="VM406_10965"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HVL76525.1"
                     /translation="MSDILNKILDVKAEEVAQAKKHCDLASLRRDVESDREARAALRG
                     FETALRTKIGAGQAGVIAEVKKASPSKGVLRADFRPAEIAASYAEHGAACLSVLTDEK
                     FFQGAPEYLQQARAACALPVLRKDFMVDLYQVYQARAWGADCILLIVAALDQGLMADL
                     EACAHELGMGVLVEVHDGEELSAALKLRTAMLGINNRNLRTFETSLQTTLGLLPRIPG
                     DKLVVTESGILKPDDVKLMRDADVHAFLVGEAFMRAPDPGQELARLFG"
     gene            complement(3435..5237)
                     /locus_tag="VM406_10970"
     CDS             complement(3435..5237)
                     /locus_tag="VM406_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HVL76526.1"
                     /translation="MKQTVRYAIGIKDAAAHLFEVVIAVSNSNPAGQTFSLPAWIPGS
                     YMIREFARNIVCIEAACGNTRIPLSKLDKHTWRAAPCSGELLVRYEVYAWDLSVRAAH
                     LDQTHGFFNGTSVFLRVHGQEHLPHVVDLRQPEGDMFAGWRVATSLPELKARRYGFGT
                     YIAGDYDDLIDHPVEMGTFALATFNAHGAAHDVVITGRVPNLDMARLSADLKKICEAQ
                     IALFEPKSRRAPVRRYVFMTLAVGDGYGGLEHRASTALICSRGDLPVKGQKEMSDGYR
                     TYLGLCSHEYFHTWNVKRIKPAVFAPYELAAETYTSLLWLFEGFTSYYDDLMLVRSGV
                     IDEQAYLKLVAKTVNGVLRGSGRTRQSVAESSFDAWTKYYRQDENAPNAIVSYYAKGA
                     VIGLALDLTIRAETRGRRSLDDAMRLLWQRYGRDFYEGGGRGVTEAEAESVFEEVAGI
                     RLRRFFDRYVRGTEDLPLAKLLQPFGVSWSDKRKTNKASLGVRTARDGNDCKLANVYE
                     GGAAHLAGLSAGDILVALDGIRVTASNLDALLGRYAVGQTVEAHAFRRDELIRFSVAL
                     LGDEAPQVSLSIESKPTLAARMRAGWLGSAEKAR"
     gene            complement(5234..5833)
                     /locus_tag="VM406_10975"
     CDS             complement(5234..5833)
                     /locus_tag="VM406_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HVL76527.1"
                     /translation="MQGRDIVGILLAAGRGTRFDANGRRNKLLARIDNTPVVALAARS
                     LRAALGRVIAVVRVDDSDTAAILADEGCTVLPIDSSDAGMSVSLRCALEHTKNADGWV
                     VALGDMPYVRPGTSRALAQALATGADIAAPYFQGRRGNPVAFGRDHFAALMQLQGDQG
                     ARALLAAHPLARVDVDDPGIHLDIDTDADLARPANENQR"
     gene            complement(5889..>6084)
                     /locus_tag="VM406_10980"
     CDS             complement(5889..>6084)
                     /locus_tag="VM406_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HVL76528.1"
                     /translation="GVDSVMAPRERRVAAWDALARELDAGKIEAITQDITLAEAIERA
                     SALLAGQVRGRLVVNVNAQA"
BASE COUNT         1075 a         1939 c         2038 g         1032 t
ORIGIN      
        1 gtgtatgccg accctgcaca tccggaagcg tattcacgcg cgcggcagcg cctgaaggac
       61 ttgcgcgcga tgctgcgccg ggcggtcgac gtgccggtga cgtcagcgtc ggtgcgcacc
      121 gagacgatcc gcgattttgc gaaggccgcc tacctcaagg cggtggaacg cgcgaaggaa
      181 tacatcatgg cgggcgacct gatgcaggtg caggtcggtc agcgcctgaa gaagccgtac
      241 gtggatgcgc cgctgtcgct gtatcgcgcg ctgcgctcgc tgaatccgtc gccgtacatg
      301 tacttctaca atttcggcga catacagatc gtcggcgcct cgccggaaat ccttgtgcgc
      361 aacgagcagg ttgcggacgg ctcgcgcaag gtgacgatac gcccgctggc cggtacccgt
      421 ccacgcggcg cgacgccgga aagcgatgcg cagctggcca aggaactcct ggccgatccg
      481 aaggagatcg ccgagcatgt aatgctgatc gacctcgcgc gcaacgacat cggtcgcatc
      541 gccgagaccg gcagcgtcaa ggtgacggac aagctggtga tcgagaagta ttcgcacgtc
      601 cagcatatcg tttcaaacgt cgagggcacg ctccggcccg gcatgtcgaa cctggacgtg
      661 ctgaaggcga ccttcccggc cggcacgctc tccggcgcgc cgaaggtgcg ggcgatggag
      721 atcatcgacg agctggagcc ggtcaagcgc ggcatctatg gcggcgcctg cggctacctg
      781 tcgttcggcg gcgagatgga cctggcgatc gcgatccgca ctggcgtcat caaggatggc
      841 atgctctacg cacaggcagc ggccggagtc gtggccgatt ccgtgccgga aatggagtgg
      901 caggaaaccg agaacaaggc gcgcgcggtg ctgcgcgcgg ccgagcaggt gcaagacggg
      961 ctggatggag gaatctgaca tgctgctgat gatcgacaac tacgactcct tcacctacaa
     1021 cctcgtccaa tatttcggcg agctgggcga ggatgtgcgc acctaccgca atgacgagat
     1081 cacgctggaa cagattgccg agctgaaccc ggaccgcatc tgcatttctc cgggcccctg
     1141 cacgccgaac gaagcaggcg tttcggtgcc ggtgctgaag gaattcggcg gcaaagtgcc
     1201 gattctcggc gtgtgcctcg ggcaccagag catcggcgcg gcattcggcg gacaagtggt
     1261 gcgtgccagg caggtcatgc acggcaagac ttccgtgatc aagcattccg gcgtgggcgt
     1321 attcaaggat ttgccggatc cgtataccgt catccgctat cactcgctgg caattgcgcg
     1381 cgaaagcctg ccggattgcc tcgagatcac cgcctggacc gaggatggag agatcatggg
     1441 cgtgcgccac aagacgctgg cgatcgaggg agtgcagttc caccccgaat cgatcctgtc
     1501 cgagcacgga catgcgctgc tgaagaactt cctgactgcc tgacatccga tcccttccgc
     1561 ccttcagccg gaaggctgtg cggatcaaag cgaggagaac ccgacatgcc gattaccccg
     1621 caacaagcgc tcttgcgctg catcgagcac cgcgagatct ttcatgacga gatgctttcc
     1681 ctgttccgca agatcatggg cggcgagatg tcgccggtga tgatcgcggc cctgaccatg
     1741 ggcctgcgcg tgaagaagga aaccatcggc gagatcgcgg cggcggcgca ggtgatgcgc
     1801 gagttcgcga ccaaagtccc ggtggaagac accacgaagc tgctcgacat cgtcggcacc
     1861 ggcggcgacg gcgcgcatac gttcaatatt tcgacggctt cgatgttcgt tgcggcagcg
     1921 gccggcgcgc gcgtggccaa gcatggcggg cgcagcgtgt cctcgtcctc cggcagcgcc
     1981 gacgtgctgg agtcgctcgg cgcgaacatc aacctgaagc cggagcaggt ggcggcatcg
     2041 atcaaggaaa ccggcatcgg gttcatgttt gctccgaacc accatgcggc gatgaagcat
     2101 gcggcgcccg tgcgcaagga actgggcgtg cgcaccatct tcaacatcct cggcccgctg
     2161 acgaatccgg cgggggcacc aaatatcctg atgggcgtgt ttcatccgga tctggtcggg
     2221 atccaggtgc gcgtcctgca gcgtctcggc gcgcagcatg cggtggtggt gtgggggcgc
     2281 gacaacatgg atgaagtgtc gctgggcgcg gggacgatgg tcggcgagct ggtcgacggc
     2341 gagatccgcg agtacgacat ccatccggaa gacttcggac tgcagatgat ggcaagccgc
     2401 aacctgaagg tgtcgggcgc ggaggaatcg aagctgcgca ttctcgaggc gctcgacaac
     2461 gtgcccgggc cggtgcgcga catcgtgatt ctcaacgccg gcactgcgct gtatgcggca
     2521 ggcgtggcaa gctcgatcgg cgacggcctg ctgaaggcgc gcgagacgat cgcctcgggc
     2581 gcggcccgca gcaagctcga acagttcgtc caggtcacgc gccagctcgg cgccctcaac
     2641 gcataagcac gcccatgtcc gacattctca acaagatcct cgacgtcaag gccgaagaag
     2701 tcgcccaggc gaagaagcat tgcgacctgg ccagcctgcg ccgcgacgtg gagagcgacc
     2761 gcgaggcgcg cgccgcgctg cgcggcttcg agacggctct gcgcacgaag atcggcgccg
     2821 ggcaggctgg cgtgattgcc gaagtgaaga aggcgtcgcc gtcgaaaggc gtgctgcgcg
     2881 cagacttccg tccggccgag atcgcggcca gctatgccga gcacggggcg gcctgcctgt
     2941 cggtgctcac ggacgagaaa ttcttccagg gtgcgccgga gtatctgcag caggcgcgcg
     3001 cggcctgcgc gctgccggtg ctgcgcaagg atttcatggt ggacctgtac caggtgtatc
     3061 aggcgcgcgc ctggggcgcc gactgcatcc tgctgatcgt ggcggcgctc gaccagggtc
     3121 tgatggccga tctggaggcc tgcgcgcatg agctcggcat gggtgtgctg gttgaggtgc
     3181 atgatggtga ggaactgtcg gccgcgttga aactgcgcac ggcaatgctc ggcatcaaca
     3241 accgcaacct gcgcacgttc gagacttcgc tgcagacgac gctcggcctg ctgccgcgaa
     3301 ttcccgggga caagctggtg gtgaccgagt ccggcatcct gaagccggac gatgtgaagc
     3361 tgatgcgcga cgccgacgtc catgccttcc tcgtcggcga agccttcatg cgcgccccag
     3421 acccggggca ggaactagcg cgccttttcg gctgagccca gccagccggc gcgcatccgc
     3481 gccgccaggg tgggtttgct ctcgatggac agactcactt gcggcgcctc gtcgccgagc
     3541 agcgcgacag agaaccgaat cagctcgtcg cgccggaacg catgtgcctc gaccgtctgg
     3601 cccaccgcgt agcggccgag cagggcgtcg agattgcttg ccgtcacgcg aataccgtcg
     3661 agcgcgacca ggatgtcccc ggccgacagg cctgccagat gcgcggcccc gccctcgtac
     3721 acgtttgcca gcttgcagtc attgccgtca cgcgcggtgc gtacaccaag actggccttg
     3781 ttcgtcttgc gcttgtcgct ccagctgacg ccgaacggct gcagcagctt ggcaagcggc
     3841 agatcttccg tgccgcgcac gtagcgatcg aagaatcgcc tgagcctgat gcccgccact
     3901 tcctcgaaca ctgactccgc ctcggcttcc gtgacgccgc gtccgccgcc ttcgtagaag
     3961 tcgcgcccgt agcgttgcca gagcaggcgc atcgcgtcgt cgagcgaacg cctgccgcgc
     4021 gtctcggcgc gaatggtgag atccagcgcg aggccgatca cggcaccctt cgcgtagtag
     4081 ctgacgatgg cgttcggagc gttctcgtcc tggcggtagt acttggtcca tgcatcgaag
     4141 ctcgattcgg ccacgctctg acgcgtgcgg ccactaccgc gcagcacgcc gttgaccgtc
     4201 ttggccacca gcttcaaata cgcctgctcg tcgatgacgc cgctgcgtac gagcatcagg
     4261 tcgtcgtaat agctggtgaa tccttcgaac agccacagca gcgacgtgta ggtctccgcg
     4321 gccagctcgt acggggcaaa caccgccggc ttgatgcgct tgacgttcca ggtgtgaaag
     4381 tactcgtgac tgcacaggcc caggtaggtg cgatagccat cgctcatctc tttctgccct
     4441 tttaccggca ggtcgccgcg cgagcagatc aacgcagtgg aggcgcggtg ctcgaggccg
     4501 ccatagccgt cgccgacggc gagcgtcatg aatacatacc ggcgtaccgg cgcgcggcgc
     4561 gacttcggtt cgaacagcgc aatctgcgct tcgcagatct tcttcaggtc cgcgctcagc
     4621 cgggccatgt cgagattcgg gacgcggccg gtgatcacca catcgtgcgc ggccccgtgt
     4681 gcattgaacg tggcgagcgc aaacgtgccc atctccaccg gatggtcgat caggtcatcg
     4741 tagtcgcccg cgatgtaggt gccgaagcca tagcggcgcg ccttcagttc cggcagggac
     4801 gtggccaccc gccagccggc aaacatatcg ccttcgggct ggcgcagatc gaccacgtgc
     4861 ggcagatgct cctgcccgtg cacgcgcaga aacacgctgg tgccattgaa gaagccgtgc
     4921 gtctggtcca ggtgggccgc gcgcaccgac agatcccatg cataaacctc gtagcggacc
     4981 agcagctcgc cgctgcaagg cgcggcgcgc caggtgtgct tgtcgagctt ggacagcggg
     5041 atacgggtgt tgccgcaggc cgcttcgatg cacacaatgt tgcgcgcgaa ctcgcgaatc
     5101 atgtagctgc cggggatcca tgccggcagc gaaaaggtct ggcccgccgg gtttgaattg
     5161 gaaacggcga tgacgacttc gaacaaatgg gcggcggcat ccttgatgcc gatcgcatag
     5221 cggacggttt gcttcatctt tgattttcgt ttgcgggacg cgcgaggtcc gcgtcggtgt
     5281 ctatgtcgag gtgaattccg gggtcgtcca catccacgcg cgcaagcgga tgcgcggcga
     5341 gcagcgcgcg ggcgccctgg tcgccctgca gctgcatcag ggccgcgaaa tggtcgcggc
     5401 cgaacgcaac gggattgccg cgccggccct ggaagtacgg cgcggcgata tctgctccgg
     5461 tagcaagcgc ctgtgccagc gcgcgcgagg tgcccggccg cacgtagggc atgtcgccaa
     5521 gcgcgacgac ccacccgtcg gcgttctttg tgtgttcaag cgcgcatcgc agcgagacgc
     5581 tcatgccggc atccgacgag tcgatcggga gaacggtgca gccttcgtcg gccaggatgg
     5641 ctgcagtgtc gctgtcgtcc acgcgcacca cggcgatgac cctgcccagc gccgcgcgca
     5701 ggctacgggc tgcaagggcg accaccggcg tgttgtcgat gcgcgccagc agcttgttgc
     5761 gcctgccgtt ggcgtcgaag cgtgtgccgc gtcctgcagc cagaaggatg ccgacgatat
     5821 cgcggccctg catcagcggc gcatccgtgc ggcattggcg ccgatgccgc gcgcgttgcg
     5881 gtgggtgctc atgcctgcgc gttcacattg acgaccaggc gaccgcgcac ctggcccgcc
     5941 agcagcgcgg atgcgcgctc gattgcttcg gcgagcgtga tgtcctgcgt gatcgcctcg
     6001 atcttgcctg catcgagttc ccgcgccagc gcatcccatg cggcgacgcg gcgctcgcgc
     6061 ggcgccatca cgctgtcgac gcca
//
LOCUS       DATLGF010000356         2915 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_230306, whole genome shotgun sequence.
ACCESSION   DATLGF010000356 DATLGF010000000
VERSION     DATLGF010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230306"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /gene="pal"
                     /locus_tag="VM406_10985"
     CDS             <1..395
                     /gene="pal"
                     /locus_tag="VM406_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HVL76529.1"
                     /translation="ARTIAPVTAASTDPLNDPKGILARRSVYFDYDSFIVKDEFKPVL
                     AAHAQYLTANRGRRIVIQGHTDERGGREYNLALGQKRAEAVLRALALLGVQESQMEAV
                     SFGKEKPRALGSDEASWAENRRGDLVYQ"
     gene            399..1148
                     /gene="ybgF"
                     /locus_tag="VM406_10990"
     CDS             399..1148
                     /gene="ybgF"
                     /locus_tag="VM406_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HVL76530.1"
                     /translation="MKRALRSHLGAALVAAFTLAPLAAQAQLFADDEARRAILQLRER
                     VNNMQNEINAKMDKTGTLDLHNQNEALRQEIARLRGQIEVLTNELANTQKRQKDFYVD
                     LDARLRKLEPQKVVVDGREITVEQAEQRAYDAALTYFREGEYRNAAASLYDFTRRYPQ
                     SGLVPSAQYWLGNTYYAMRDYQNAIAAHQVVLDRFPTDSRAPEALLNIASCHLELKNR
                     DAARTALETLLSRYPDSPAAATARERLQNLR"
     gene            complement(1205..2116)
                     /locus_tag="VM406_10995"
     CDS             complement(1205..2116)
                     /locus_tag="VM406_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HVL76531.1"
                     /translation="MILPVLIFLAVTLGLVGAYLWLTPSRAEQRLHTLLEPRKSTNWV
                     ATAVQVVGPFAKLSIPEGDWERAPLRMKFINAGIRQPDARLVYFGAKTLLPLLAAGLA
                     FILLRAWSAGSGLGLVLWMMIAALVGCYLPNLVLHWLVKRRKREVFESFPDAADLMLV
                     CVEAGLGLDAALARVAEEMKMKSLALAEELHLTNLEMRAGGTREQALRNLATRTGVEE
                     VSMFSTMLSQADKFGTSIGDSLRVFSDDLRHKRQSRAEELAAKVPTKMLFPLVVCIFP
                     SIVMVIMGPAVIQIVRTILPMIGMGEN"
     gene            complement(2133..>2915)
                     /locus_tag="VM406_11000"
     CDS             complement(2133..>2915)
                     /locus_tag="VM406_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003079634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HVL76532.1"
                     /translation="VPAMERLVAALPKARGLQSFIAQAGLNWTVSHLMLTSSALGGFT
                     YFGLAMLAHQPGLFAAVAGLLAAMLPFLLVQRCRARRLARIEHQLPDALDLMIRALRS
                     GHAFSSALQMVGDEMPEPVANEFRIVHDEVSFGVSLQQALINLSRRVPLTDVRYFVVA
                     VLIQRESGGNLTEVLGNLSRLVRDRLKFNAKVRVLSSEGRMSAWVLGVMPFALAGVMN
                     LVNPDFMSLMWTDPIGIAILQYLFILMVAGIIMLKRIIRIRV"
BASE COUNT          603 a          968 c          881 g          463 t
ORIGIN      
        1 atgcgcgcac gatcgcaccc gtgacggctg cgtcgaccga tccgctgaac gacccgaagg
       61 gcatcctggc ccgccgcagc gtctacttcg actacgacag cttcatcgtc aaggacgaat
      121 tcaagccggt gctggccgcc catgcgcaat acctgaccgc caaccgcggc cgccgtattg
      181 tgatccaggg ccacaccgac gagcgcggag gccgcgagta caacctcgcg ctgggccaga
      241 agcgcgccga ggccgtgctg cgcgcgctgg cgctcctggg tgtgcaggaa tcccagatgg
      301 aagccgtatc gtttggcaag gaaaaaccgc gtgcgctggg cagcgacgaa gcctcctggg
      361 ctgagaaccg ccgcggcgac ctcgtctacc agtaagccat gaaacgcgcc ctccgctccc
      421 acctcggcgc ggccctcgtg gccgcattca cgctcgcgcc tctggccgcc caagcccagc
      481 tgttcgcgga cgacgaggcg cgccgcgcaa tcctgcaatt gcgcgagcgc gtcaacaaca
      541 tgcagaacga gatcaatgcc aagatggaca agaccggcac cctcgacctg cacaaccaga
      601 acgaggcgct gcgccaggag atcgcccgct tgcggggcca gatcgaagtc ctgaccaacg
      661 agctggccaa cacgcagaag cggcagaagg acttctacgt ggatctcgac gcgcgcctgc
      721 gcaagctgga accccagaag gtggtggtgg acgggcgtga aatcacggtc gagcaggccg
      781 aacagcgcgc ctatgacgcc gcgctgacct acttccgtga aggcgagtac cgcaatgcgg
      841 ccgcgtcgct gtatgacttc acgcgccgct atccgcaatc cggcctcgtt ccgtcggcgc
      901 agtactggct cggcaacacc tactatgcga tgcgcgatta ccaaaacgcg atcgcggcgc
      961 accaggtggt gctggaccgc ttcccgaccg attcacgtgc gccggaagcc ctgctcaaca
     1021 tcgccagctg ccacctggaa ctgaagaaca gggatgccgc gcgcacggcg ctggaaacgc
     1081 tgctgtcgcg ctatccggac tcgccggcgg cggccaccgc gcgcgagcgg ctgcagaacc
     1141 tgcggtaagc gcccagcacg ccagcagaac ggcgcttgtg cgccgttctt ttttctcgcc
     1201 ttgctcagtt ttcgcccatg cctatcatcg gcaggatcgt gcgcactatc tgaatgaccg
     1261 ccgggcccat gatgaccatg acgatggacg gaaagatgca gaccacgagt ggaaaaagca
     1321 tcttggtagg caccttcgcc gcgagttcct ccgctctgct ctggcgcttg tgccgaagat
     1381 cgtcggagaa cacgcgtagc gaatcaccga tgcttgtgcc gaacttgtcc gcctgcgaca
     1441 gcatcgtcga gaacatcgat acctcttcca cgcccgtgcg cgtggccaga ttacgcagtg
     1501 cctgttcgcg cgtcccgccg gcgcgcattt cgagattcgt caggtgcagt tcttcggcca
     1561 aggccaggct tttcatcttc atttcctcgg caacgcgcgc gagcgctgca tccagtccca
     1621 ggcccgcttc gacgcagacc agcatcagat ccgccgcgtc cggaaaactt tcgaagacct
     1681 cgcgcttgcg acgcttcacc agccagtgca gcacgaggtt cggcagataa cagccgacga
     1741 gcgcggcgat catcatccaa agaacgaggc ccagaccgct gccggcactc catgcgcgca
     1801 gcagtatgaa ggcgaggcca gccgcaagca ggggcaacag cgtcttggcg ccgaagtaaa
     1861 caagccgggc atccggctgg cggatgccgg cgttgatgaa cttcatgcgc agcggagccc
     1921 gctcccagtc gccctccggg atcgacagct ttgcgaacgg ccccacgacc tgtacggcag
     1981 tggccaccca gttcgtactc ttgcgtggct cgagcagcgt atgcaggcgc tgctccgcgc
     2041 ggctcggcgt caaccaaaga taggcaccta cgaggccgag cgtaacggca aggaagatca
     2101 ggacgggaag aatcacggct tttctccttg cgtcagacgc ggattcggat gatccgtttc
     2161 agcatgatga tgccggcaac catcaagatg aacaggtatt gaagaatggc gatgccgatc
     2221 ggatcggtcc acatcagcga catgaaatcc ggattgacca gattcatcac gccagcgagc
     2281 gcgaagggca tgacgccaag cacccatgcg gacatccgcc cttccgacga cagcacccga
     2341 accttggcgt tgaacttgag ccggtcgcga accaggcggc tcaggtttcc caacacctcc
     2401 gtcagattgc cacccgactc gcgctgaatc aggaccgcga cgacgaagta gcgcacgtcg
     2461 gtgagaggta cgcgtcggct gagattgatc agcgcctgct gcagtgagac gccgaagctc
     2521 acttcgtcat gcacgatccg aaattcgttt gcgaccggct ccggcatttc atctccaacc
     2581 atctgcaggg ccgacgaaaa cgcatgcccc gaccgaagcg ctcgtatcat cagatccagt
     2641 gcatcgggca gttggtgctc gattcgcgcc aaccggcgcg cgcgacagcg ctgcacaaga
     2701 aggaaaggga gcattgcagc gagcaagccg gccaccgcgg cgaacagtcc cggctggtgg
     2761 gcgagcatgg cgaggccaaa gtatgtgaaa cctccaagcg ccgaactggt cagcatcagg
     2821 tgcgagactg tccagttcag cccggcctgg gcgatgaacg actgcaggcc gcgtgccttc
     2881 ggcagcgcgg cgacgagccg ttccatcgcc ggcac
//
LOCUS       DATLGF010000357         3132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_140000, whole genome shotgun sequence.
ACCESSION   DATLGF010000357 DATLGF010000000
VERSION     DATLGF010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140000"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="VM406_11005"
     CDS             <1..914
                     /locus_tag="VM406_11005"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVL76533.1"
                     /translation="SEVFDYLVVATGHFSTPNVPWFEGLDGFRGSVMHAHDFRGADQF
                     VGKDLLLVGGSYSAEDIGVQCYKHGARSVTISYRSQPLGFKWPKGMREVPLVKRFDGH
                     KAYFVDGTSCRFDAVVLCTGYQHKYPFLPKELRLETNNRLYPKNLYKGVVWQANPRLF
                     YLGMQDQYYTYNMFDAQAWYVRDLILGRMSTPGREARQADINRWVRKEEAIETAFDAV
                     DFQTEYVCDLMSQTDYPGFEAEEVAKLLKQWLRDKEENILTYRDKPFRSVVTGTMAPV
                     HHTPWLEEMDDSAARFLDSSLSRHEQA"
     gene            complement(999..2030)
                     /gene="mnmH"
                     /locus_tag="VM406_11010"
     CDS             complement(999..2030)
                     /gene="mnmH"
                     /locus_tag="VM406_11010"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HVL76534.1"
                     /translation="MLDFNAYGLIIDARSPREYEEDHIPGALNLPVVDNDEYAEVGTL
                     HRTDPMGAYQIGVVYSLNNIARHVRETVAAYDRKTRVLVYCFRGGKRSKLWFDALDTI
                     GYRVDRLPGGWKGYRRHVIEQLTTLPAQLRYLVLSGATGCGKTRLLAALQREGAQVLD
                     LEGLAVHRGSIIGAVPGQPQPSQKLFETRLLHTLQGFDPGKPVWVEAESKMIGRVRLP
                     PALLDGMRMGETVRVDAAMEQRVQLWREDFGHFEQDPKALIERLAYLKPLIGGEEFQA
                     WESLAEAGEVAALFERLMRNHYDPAYARSLKRHFPNHERAPSVMLEDLSQQGLKPVAQ
                     ALREQFEAR"
     gene            2161..>3132
                     /gene="selD"
                     /locus_tag="VM406_11015"
     CDS             2161..>3132
                     /gene="selD"
                     /locus_tag="VM406_11015"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HVL76535.1"
                     /translation="MTDSTASTSVPPSAIRLTSFSHGGGCGCKIAPGVLADILRHSSG
                     FPVPKELMVGIETADDAAVYRLNDKQALIATTDFFMPIVDDPFDFGRIAATNAISDVY
                     AMGGTPIMALAIVGMPVNKLPPEVIGAILRGGESVCAQAGIPVAGGHTIDSVEPIYGL
                     VALGLAHPERIRRNAAAREGDVLVLGKPIGVGVLSAALKKNLLDAAGYRTMIDCTTRL
                     NTPGPLLAELDGVHAMTDVTGFGLLGHALEMARGAGLDIALDAAAVPLLPQVAALAEA
                     GCVTGASARNWAGYGQSVMLGERIGPIQQALLTDPQTAGGLLVACAPD"
BASE COUNT          522 a         1023 c         1038 g          549 t
ORIGIN      
        1 attccgaggt cttcgactac ctcgtggtcg ccaccggcca cttctccacg ccgaacgtgc
       61 cgtggttcga ggggctcgac ggcttccgtg gcagcgtcat gcacgcccat gatttccgcg
      121 gcgccgacca gttcgtcgga aaggacctgc tgctcgtcgg cggcagctat tcggcggaag
      181 acatcggcgt gcagtgctac aagcacggcg cgcgctcggt caccatcagc tatcgttcgc
      241 agccgctggg cttcaagtgg ccaaagggga tgcgagaagt cccgctggtc aagcgcttcg
      301 atgggcacaa ggcgtatttc gtcgacggga cctcgtgccg tttcgatgcg gtggtgctgt
      361 gtaccggcta ccagcacaag tacccgttcc tgccgaagga gctgcgcctg gaaaccaaca
      421 accgcctgta cccgaagaac ctgtacaagg gcgtggtctg gcaagccaat ccgcgtctgt
      481 tctatctcgg catgcaggac cagtactaca cgtacaacat gttcgacgcg caggcctggt
      541 atgtgcgcga cctgatcctc ggccgcatga gcacgccggg gcgggaggca cgccaggcag
      601 acatcaatcg ctgggtacgc aaggaagaag cgatcgaaac ggcgttcgat gccgtggatt
      661 tccagaccga gtacgtctgc gacctgatga gccagacgga ctacccgggc tttgaagccg
      721 aggaagtcgc caagctgctc aagcagtggc tgcgcgacaa ggaggaaaac atcctcacct
      781 atcgggacaa gcccttccgt tcggtcgtga ccggcacgat ggcgccggtg catcacacgc
      841 catggctgga ggaaatggac gatagcgccg cgcgctttct cgattcgtcc ttgagcaggc
      901 acgagcaggc ctgaggtcga ctcggcctgg gcgcggtcat tacgcaaagg cggcgcacgt
      961 cgcgccgtct ttgccggccg cttctacagg cgccggacct agcgcgcttc gaactgctcc
     1021 cgcaatgcct gcgccaccgg cttgagtccc tgctgcgaca aatcctccag catcactgac
     1081 ggcgcacgct cgtggttggg gaagtggcgc ttgagcgagc gcgcgtaggc ggggtcgtag
     1141 tgattgcgca tcaggcgctc gaacagggcc gcgacttcac cggcctcggc caggctttcc
     1201 caggcctgga attcttcacc gccgatcagc ggcttcaggt acgcaagccg ctcgatcagg
     1261 gccttcggat cctgctcgaa gtgcccgaag tcttcgcgcc atagctgcac gcgctgttcc
     1321 atcgccgcat ccacgcgcac cgtctcgccc atgcgcatgc cgtccagcag cgccgggggc
     1381 aggcgcacgc ggccgatcat cttgctttcc gcctcaaccc ataccggctt gccgggatcg
     1441 aagccttgca gcgtgtgcag cagccgggtt tcgaacagct tctgcgaagg ctgcggctgg
     1501 ccgggaaccg cgccgatgat cgaaccgcga tgcaccgcca ggccttcgag gtccagcacc
     1561 tgcgcgcctt cgcgctgcaa ggccgcaagc agccgggtct tgccgcaacc ggtggcgccc
     1621 gagagcacca ggtagcgcag ctgcgcggga agcgtagtga gctgttcgat cacatggcgc
     1681 cggtagcctt tccatccgcc gggcagccgg tcgacgcggt agccgatggt gtcgagcgca
     1741 tcgaaccaca gcttgctgcg cttgccgccg cggaagcagt acaccagcac acgcgtcttg
     1801 cggtcgtacg ccgcgacggt ttcgcgcacg tggcgtgcaa tgttgttcag ggaatagacg
     1861 acgccgatct gatacgcgcc catcgggtcc gtgcggtgca gggtgccgac ttcggcgtat
     1921 tcgtcgttgt cgaccaccgg cagattgagt gcgccgggga tgtgatcttc ctcgtattcg
     1981 cgcgggctgc gcgcgtcgat gatgaggccg taggcattga aatcaagcat tgcgacattg
     2041 tagccgcgtg cgcgatgggg gatcgtgtgc agcagcctcc cagccctgcg aaagccgggg
     2101 cgacgggctg cgtgcaaggt tttgccggat aatcgggtcc gttttccttc gaggcccgcc
     2161 atgaccgatt ccaccgcgtc cacatccgtc cccccttccg cgatccgcct gacgtcgttc
     2221 tcccatggcg gcggctgcgg gtgcaagatc gcgccaggcg tgctggccga tatcctgcgc
     2281 cattcgtccg ggtttccagt tccgaaggaa ttgatggtgg gtatcgagac ggccgacgat
     2341 gccgccgtct accgactcaa cgacaagcag gcgctgatcg ccaccaccga tttcttcatg
     2401 cccatcgtcg acgacccgtt cgacttcggc cgcatcgccg caaccaacgc gatttccgac
     2461 gtgtatgcga tgggcggcac accgatcatg gcactggcga tcgtcggcat gccggtcaac
     2521 aagctgccgc cggaggtgat cggggcgatc ctgcgcggcg gggagtcggt ttgcgcgcag
     2581 gcggggattc ccgtggcagg tggccacacc atcgattccg tcgagccgat ctacgggctg
     2641 gtggcgctgg gcctcgcgca tccggagcgc atccgccgca atgcggccgc acgggaaggc
     2701 gacgtgctgg tgctgggcaa gccgatcggc gtaggcgtgc tttccgccgc actcaagaag
     2761 aacctgctcg acgctgccgg ctaccggacg atgatcgact gcacgacccg gctcaacacg
     2821 ccgggaccgc tgctcgcgga actggacggc gtgcacgcga tgacggacgt gaccggcttc
     2881 ggcctgctcg gtcatgcgct ggaaatggcg cgcggcgcgg ggctggacat cgctctcgac
     2941 gccgccgcgg tgccgctgct gccgcaggtc gcggcgctgg cggaggctgg ctgcgtgacc
     3001 ggcgcttcgg cgcgcaactg ggccggctac gggcaatccg tcatgttggg cgagcggatc
     3061 ggcccgatcc agcaggcgct gctgaccgac ccgcagacgg ccggcgggct gctggtggcc
     3121 tgcgcgccgg ac
//
LOCUS       DATLGF010000358         4987 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_119966, whole genome shotgun sequence.
ACCESSION   DATLGF010000358 DATLGF010000000
VERSION     DATLGF010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119966"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VM406_11020"
     CDS             complement(<1..665)
                     /locus_tag="VM406_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76536.1"
                     /translation="MWRTLPLLVALFLVSNSTLAQHRHASLQNDDRSLPRKVIIIPAD
                     VVVHEMSVGGILEKAPEWARQSSDNLTRAMREVAEDIRHFTIVQAPTLTEEEQDRLDE
                     SIATFMTVGTTAHQTLLWGGAAWAHKRTEFDYTLGPGLAFLREKTGADAAILMAGDDI
                     VSSDGRKAAVVVGALLGVGLATGRSIALTSVVDLQTGDLLWMHYDESMSKDLKNYDSA
                     KTMV"
     gene            complement(769..1560)
                     /locus_tag="VM406_11025"
     CDS             complement(769..1560)
                     /locus_tag="VM406_11025"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HVL76537.1"
                     /translation="MKTMHDELPYVLVSREGGIATLTLNRGDKFNPLSEEMMDALQRE
                     LDALKADAGVRVVVLAAAGRAFCAGHDLKQMRAQPSLDYYKRLFAQCSKMMLTIQRMP
                     QPVIARVQGIATAAGCQMVAMCDLAVASSEARFAVSGVNYGLFCSTPSVGLSRNVSRK
                     QAMEMLLTGEFIDAATARERGLVNRVVEPNELDAEVGRLAASILAKPAVAVAMGKELF
                     YKQLEMGIDAAYQLAGQTMACNMMDEAALEGVQAFIEKRRPQWST"
     gene            complement(1544..2461)
                     /gene="ylqF"
                     /locus_tag="VM406_11030"
     CDS             complement(1544..2461)
                     /gene="ylqF"
                     /locus_tag="VM406_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HVL76538.1"
                     /translation="MSIQWFPGHMNAARKKAAESMEKTDVVIEVLDARVPQASSNPMI
                     DEMRRHRQRPCLKILNKSDLADPAATKAWLAHYAAQKDVHAVALSCKNPADVAKVTGL
                     AQKIAPHRGTALKPLRMMIMGIPNVGKSTLMNALLKKRVAAVGDEPAVTKAQQRLYLG
                     NNMVLVDTPGMMWPKIEHPTDGLMLAASHAIGVNAVIEEEVATFLADLLLARYAQLLA
                     KRYGFKTEGMDGVAVVEGIAARRGYRQKGGELDFEKAAHTLLHDYRTGALGRISLETP
                     ESRAQLLASYQPPPSLGLAQTTDEDEDHA"
     gene            2591..3511
                     /locus_tag="VM406_11035"
     CDS             2591..3511
                     /locus_tag="VM406_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HVL76539.1"
                     /translation="MFAKVHAVARQVGQVVVGKDVQIRQALVCLLAGGHLLIEDLPGV
                     GKTTLAHALAISLGLKFNRLQFTSDLLPADVIGISVFDREKNGFVFHPGPVFTQVLLA
                     DEINRATPKTQSALLEVMEERQVTAEGLTRALPEPYFVIATQNPSHQVGTFQLPESQL
                     DRFLMCISLGYPDAASERALLLGEDRRAMLRTLPTAMRPEELLDARAALKSIHVSEAL
                     AGYVQSLAQASRRNGMFAEGLSPRAAIALLQAARAWAAMEGRNHVIPEDVQAVLVPVA
                     AHRLRPLKSGGSTALASRDLVLQLMKSVPV"
     gene            3508..4527
                     /locus_tag="VM406_11040"
     CDS             3508..4527
                     /locus_tag="VM406_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HVL76540.1"
                     /translation="MTPQLSLYARIEHGLRRHASRWLSRRRAPAPGTLFLSQRRIFIV
                     PTHPGLLYGAMLATLFIGSVNYNLNLGFVLTFLLAAVGFVDMHLTFRNLAYLNLVAGR
                     AAPVFAGEDAQFELQLVNDRRRDRYAIWLDFVHETGEPDPAHAVDVAANTTATVVLAT
                     RAPERGWLPAPRVRLQTRFPLGLLRAWTYWRPDARVLVYPRPEDDAPPLPLEGHDGAT
                     ENGMEGHDDFAGIRGYQSGDSLKHLAWRQIARLDPALGGNLVTKQFEGGAGGEILLDL
                     ARLPVQMGLEAKLSRLTRWVLEAEARGIDYALRLGTTTIPASSGVAHRNDCLRALALY
                     GKEAS"
     gene            4524..>4987
                     /locus_tag="VM406_11045"
     CDS             4524..>4987
                     /locus_tag="VM406_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HVL76541.1"
                     /translation="MRERLAALLRAPLSADKVNMLLLLGSCALVLLPHAAHLPLWVVP
                     ACVALMGWRAWVTFRGNRLPPRWLLWPLAILSVLAVAWTYRTIFGREAGVAMLALLLS
                     LKLLEIRAKRDLFVVLFLCFFLILASFFHSQSIASAALMLVAVVAILTTQMS"
BASE COUNT          748 a         1683 c         1634 g          922 t
ORIGIN      
        1 accatggttt tcgcgctgtc gtagtttttc agatcctttg acatgctctc gtcatagtgc
       61 atccagagca ggtcgccggt ttgcaggtcc acgactgagg tgagcgcaat tgaacgcccg
      121 gtggcaagac cgacgccgag aagcgcgcca accacgacgg ccgccttgcg tccgtcggac
      181 gagacgatgt cgtcgccggc catcaggatt gccgcgtcag ctccggtctt ctcgcgcagg
      241 aacgccagtc cggggccaag ggtatagtcg aattcggtgc gcttgtgcgc ccaggctgcg
      301 cctccccata gcagcgtctg gtgggcggtc gtgccgacgg tcatgaaggt cgcgatcgac
      361 tcgtccaacc ggtcctgctc ctcttccgtc agagtggggg cctggacgat agtgaaatgc
      421 cggatatcct cggcaacctc gcgcatggcc ctggtcaggt tgtcggatga ctggcgagcc
      481 cactccggtg ccttttccag aatgccgccg accgacattt cgtggacgac cacgtcggca
      541 ggaatgatga tgaccttccg cggcagcgac cgatcgtcat tctgcagcga ggcgtggcga
      601 tgctgcgcca acgtggaatt cgagaccaag aacaaggcaa ccagcaacgg cagcgtgcgc
      661 cacatacagc ctcccgatag atgaaggaat actcccgcgg cgcatcatat cgttcgtgca
      721 actttctgta aatttacttg aatcgctgtg ttgcaaatcg gcttcggttc aggtcgacca
      781 ttgcgggcga cgtttctcga tgaacgcctg caccccttcc agcgccgcct cgtccatcat
      841 gttgcaggcc atcgtctgcc cggcgagctg gtaggccgcg tcgatgccca tttccagctg
      901 cttgtagaac agctccttgc ccatcgcgac cgcaaccgcc ggcttggcga ggatgctcgc
      961 ggcgaggcgg ccgacttccg catccagttc gttcggctcg accacgcgat tgacgaggcc
     1021 gcgttcgcgc gcggtggccg catcgatgaa ttcgccggtc agcagcattt ccatcgcctg
     1081 cttgcgcgag acgttgcgcg agaggccgac cgaaggcgtc gagcagaaca ggccgtagtt
     1141 gacgccggag accgcaaagc gcgcctcgct ggaggccact gcgagatcgc acatcgcgac
     1201 catctggcac ccggcggcgg tggcgatgcc ctgcacgcgc gcgatcaccg gctgcggcat
     1261 gcgctggatc gtcagcatca tcttgctgca ctgggcgaac aggcgcttgt agtagtcgag
     1321 gctcggctgg gcgcgcatct gcttgagatc gtgccccgca cagaacgcgc ggccggccgc
     1381 ggcgagcacc accacgcgca cgccggcgtc ggccttcagg gcgtccagct cgcgctgcag
     1441 cgcgtccatc atctcttcgg acagcggatt gaacttgtcg ccgcgattga gcgtgagggt
     1501 ggcgatgccg ccttcgcggc tcaccaggac gtacggcagc tcgtcatgca tggtcttcat
     1561 cctcgtcggt ggtctgggcc agccccaggc tgggcggcgg ttggtagctc gcgagcagct
     1621 gcgcgcggct ttcgggggtt tccaggctga tgcggccgag cgcgccggtg cggtagtcgt
     1681 gcagcagcgt gtgcgcggct ttctcgaaat cgagctcgcc gcccttctgt cggtagccgc
     1741 ggcgcgcggc gatgccctcg accaccgcca cgccgtccat gccctcggtc ttgaagccgt
     1801 agcgcttcgc gagcagctgc gcatagcgcg ccagcagcag gtcggcgagg aaagtggcga
     1861 cttcctcctc gatcaccgcg ttcacgccga tcgcatggct ggcggcgagc atcaggccgt
     1921 cggtcggatg ttcgatcttc ggccacatca tgccgggcgt gtcgaccagc accatgttgt
     1981 tgccgaggta aaggcgctgc tgcgccttgg tcaccgccgg ctcgtcgccg accgctgcca
     2041 cgcgcttctt cagcagcgcg ttcatcagcg tggacttgcc gacgttcggt atgcccatga
     2101 tcatcatgcg cagcggcttg agcgcggtgc cgcggtgcgg cgcgatcttc tgcgccaggc
     2161 cggtcacttt cgccacgtcg gccgggttct tgcagctgag cgcgaccgcg tgcacatcct
     2221 tctgcgcggc gtaatgcgcg agccaggcct tggtggcggc cgggtcggcg agatcgctct
     2281 tgttgaggat cttcaggcag gggcgctggc gatggcggcg catctcgtcg atcatcgggt
     2341 tggaactggc ctgcggcacg cgcgcgtcca gcacttcgat gacgacgtcg gtcttttcca
     2401 tggactccgc cgccttcttg cgggcggcgt tcatgtgacc ggggaaccat tggatggaca
     2461 tgggtttcga gagggagtga caaggcgtta ttctacccgt ccgccgccgc gcgaagattt
     2521 gtcgtgcggc aacgccccgt cccgcgttct cgcataaaat cagccgctcc agcgcccttc
     2581 caggattgtc atgtttgcca aagttcatgc cgtcgcccgg caggtcggtc aggtcgttgt
     2641 cggaaaggat gtgcagatcc gccaggcgct ggtatgcctg ctcgcgggcg gccacctgct
     2701 gatcgaggac ctgcccggcg tcggcaagac cacgctcgcg catgcgctgg cgatctcgct
     2761 cggcctgaag ttcaaccgcc tgcaattcac cagcgacctg ctgccggccg acgtgatcgg
     2821 catctcggtg ttcgatcgcg aaaagaacgg cttcgtattc catcctggac cggtcttcac
     2881 gcaagtgctg ctggccgacg aaatcaaccg cgcgacgccg aagacgcagt ccgcgctgct
     2941 cgaagtgatg gaagaacggc aagtcaccgc cgaaggcctc acgcgcgcgc tgcctgagcc
     3001 ctacttcgtg atcgcgaccc agaatccctc gcaccaggtc ggcactttcc aattgccgga
     3061 gtcgcaactc gaccgcttcc tgatgtgcat ctcgctcggc tatccggatg ccgcctcgga
     3121 gcgcgcgctg ctcttgggcg aggaccggcg cgcgatgctg cgcacgctgc ccaccgcgat
     3181 gcggcccgag gaactgctgg acgcgcgcgc ggcgctgaag tccatccatg tttccgaagc
     3241 gctggccggc tatgtgcagt cgctggcgca ggcttcgcgc cgcaacggca tgttcgccga
     3301 gggcctgagc ccgcgcgcgg cgatcgcact gctgcaggcc gcgcgcgcat gggcggcgat
     3361 ggaaggccgc aaccatgtga ttccggaaga cgtgcaggcg gtgctggtgc cggtcgccgc
     3421 gcatcggctg cggccgttga aatccggcgg cagcaccgcg ctggccagcc gcgacctcgt
     3481 gctgcagctg atgaaatcgg ttccggtgtg acgccgcagc tgagccttta tgcacgcatc
     3541 gaacacggcc tgcgccggca tgcgagccgg tggctgtcgc gccgccgcgc acccgcaccc
     3601 ggcacgctgt tcctgagcca gcgccgcatc ttcatcgttc ccacccatcc cggccttttg
     3661 tacggcgcga tgcttgcgac gctgttcatc ggctcggtca actacaacct gaacctcggc
     3721 ttcgttctca cattcctgct cgccgcggtc ggcttcgtcg acatgcacct gaccttccgc
     3781 aacctcgcct atctgaacct ggtggccggg cgcgcggcgc cggtattcgc cggcgaggac
     3841 gcccagttcg agctgcaact ggtcaacgac cgccgccgcg accgctacgc gatctggctg
     3901 gacttcgtgc atgaaaccgg agaacccgat ccggcccacg cggtggatgt tgccgccaac
     3961 acgacggcga ccgtcgtgct cgccacgcgc gcgcccgagc gcggctggct accggcgccg
     4021 cgcgtgcgcc tgcagacccg cttcccgctc ggcctgttgc gcgcgtggac ctactggcgc
     4081 ccggatgcgc gggtgctcgt ctatccgcgc ccggaagacg acgcgccgcc gctgcccctg
     4141 gaaggccacg acggtgcgac cgaaaacggg atggagggac acgacgattt cgccggcata
     4201 cgcggctacc agtccggcga ttctctgaag cacctcgcgt ggcgccagat cgcgcggctc
     4261 gatcctgcgc tcggcggcaa cctcgtcacc aagcagttcg aaggcggcgc cggcggcgag
     4321 atcctgctgg acctggcgcg cctgccggtg cagatgggcc tggaggcgaa actctcgcgc
     4381 ctgacgcgct gggtgctgga agccgaggcg cgcggcatcg actatgcgct gcggctcggc
     4441 accacaacca tcccggcttc atccggcgtc gcgcaccgca atgactgcct gcgcgcgctt
     4501 gcgctgtacg gcaaggaggc gtcgtgaggg aacgcctggc cgccctgctg cgtgcaccgc
     4561 tgtcggccga caaggtcaac atgctgctgc tgttgggcag ctgcgcgctg gtgctgctgc
     4621 cgcacgcggc acacctgccg ctgtgggtgg tgccggcctg cgtggcgctg atgggctggc
     4681 gcgcctgggt caccttccgc ggcaaccggc tgccgccgcg ctggctgctg tggccgctgg
     4741 ccatactctc ggtgctggcg gtcgcgtgga cctaccgcac catcttcgga cgcgaagccg
     4801 gcgtggcgat gctggcgctg ctgctgtcgc tgaagctcct ggagatccgc gccaagcgcg
     4861 acctcttcgt ggtgctgttc ctgtgcttct tcctgatcct ggcgagcttc ttccactcgc
     4921 agtcgatcgc gagcgccgcg ctgatgctgg tggccgtggt tgcgatcctg accacgcaga
     4981 tgtcgtt
//
LOCUS       DATLGF010000359         3980 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_213587, whole genome shotgun sequence.
ACCESSION   DATLGF010000359 DATLGF010000000
VERSION     DATLGF010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213587"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1733)
                     /locus_tag="VM406_11050"
     CDS             complement(<1..1733)
                     /locus_tag="VM406_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HVL76542.1"
                     /translation="MVEKLRVGEKWYILATASPADERRRVLKHNDLFGMFDRYGDIQS
                     IGLGEEGLYLGDTCFLSHQELLIEGVRPMFLNSTVRDDNGLLVVELMNPDLHPEGSNR
                     IMKGEMHIFRAKLLWNNACFEHVRVVNFGLEQVCITLAMEFAADYKDIFEVRGYHCKE
                     RGQYLQPEVGERELLLGYRGRDQCVRKTRIRLDPKPDTLTTGRAEYRIDLPPKGEAHV
                     YVTVGCERDDEHAPVDLDYFSAFKKINQDVGTSLLVRSKVSTSDPLFNRWLDRSAADL
                     VMLNTRLEHGDYPYAGVPWYSTTFGRDGIITAREYLWIDPPLARGVLGFLADTQATEF
                     DPERDAEPGKILHEARNGELAALNEIPFRRYYGTVDATPLFVALAGAYYRRTGDLDTI
                     RRIWPNIRAALRWIDEYGDRDGDGFVEYARYTETGLAQQGWKDSHDSVFHADGTLADA
                     PIALCEVQGYVYEARQHAAELARLLGEHEVAQELLDAARSLKQRFNEKFWCEEIGTFA
                     IALDGGKRQCKVRSSNAGHTLWSGIALPEYAQRVADQLLEEDFFCGWGIRTIPRKEAR
                     FNPMAYHNGSV"
     gene            complement(1738..2790)
                     /locus_tag="VM406_11055"
     CDS             complement(1738..2790)
                     /locus_tag="VM406_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVL76543.1"
                     /translation="MKIAQIAPLFESVPPRHYGGTERVVSYLTEELVRQGHEVTLFAS
                     GDSDTAATLVAPVPVSGRPQPGWASWLPAHCVELDMVMERIAQFDVLHFHTDLLHFPL
                     VRRTGVAHVTTLHWRLDLPELAPLFHHFNDVPVVSISRQQQSPLPHARWVGTVHHGLP
                     GDQYRYGPGDGGYFLFIGRFSPEKRVDRAIEIAQHCGTPLYVAAKIDETRPDYFEKEV
                     RPLLDRPFVRYVGEIGEREKRELLGGALALLSPIDWPEPFGLVLIEALACGTPVIAYR
                     NGAVPEILDDGVTGFIVENQEQAVEAACKVHALDRRACRDAFERRFTAERMAANYLQI
                     YRALQDRHSEAAPRTR"
     gene            2947..3117
                     /locus_tag="VM406_11060"
     CDS             2947..3117
                     /locus_tag="VM406_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76544.1"
                     /translation="MNETAVFAPPREQHEPERPDFPAPEPVTPPSEPVLPETEPVVPE
                     PEPLEPEREPMQ"
     gene            complement(3159..3479)
                     /locus_tag="VM406_11065"
     CDS             complement(3159..3479)
                     /locus_tag="VM406_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HVL76545.1"
                     /translation="MSTQFEHVSIPKKANIYFGGKCISHNLYFPGGSRKTIGVLLPAT
                     VTFTTDGPEIMEIVSGHCRARIGDDGEWKEYHAGQRFRVPGQTRFDMEADEPVEYICH
                     FTHD"
     gene            complement(3641..>3980)
                     /locus_tag="VM406_11070"
     CDS             complement(3641..>3980)
                     /locus_tag="VM406_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HVL76546.1"
                     /translation="WAGAIDSVGSHTLANVCAGTRLGGAVAACGLAQGMDFPATVAPF
                     ILRGVSLLGINSVNPPLHLRLAAWEALAREVDSRIIGAIASEITLEQAIPMARELLDG
                     KVKGRVIVRL"
BASE COUNT          706 a         1276 c         1310 g          688 t
ORIGIN      
        1 cacaccgaac cgttgtggta ggccatcgga ttgaagcgcg cttccttgcg cggaatcgtg
       61 cggatgcccc agccgcagaa gaagtcttcc tcgagcagct ggtcggccac ccgctgcgca
      121 tactcgggca acgcaatgcc gctccatagc gtatggccgg cgttcgacga gcgcaccttg
      181 cactgccgct ttccgccatc gagcgcgatc gcgaaggtgc cgatctcttc gcaccagaac
      241 ttctcgttga aacgctgctt cagactgcgc gcggcatcca gcagctcttg ggcgacctcg
      301 tgctcgccga gcaggcgcgc aagctcggcc gcgtgctgcc tggcttcata gacgtatccc
      361 tgcacctcgc acagcgcaat cggcgcgtcg gcaagcgtgc cgtcggcgtg gaagaccgaa
      421 tcgtgcgaat ccttccagcc ctgctgggcc aggccggtct cggtgtagcg cgcatactcg
      481 acgaaaccgt cgccgtcgcg gtcgccatac tcgtctatcc atctgagcgc cgcgcggata
      541 ttgggccaga tgcggcgtat cgtatcgaga tcgccggtgc gcctgtagta cgcgccggcg
      601 agcgcaacga acagcggcgt ggcgtcgacg gtgccgtagt agcgtcgaaa tggaatttca
      661 ttcagtgcgg ccagctcgcc gttgcgcgcc tcgtgcagga tcttgccggg ctcggcgtcg
      721 cgctcggggt cgaactcggt ggcctgagtg tcggcgagaa atccgagcac gccgcgcgcc
      781 agcggcggat cgatccacag gtattcgcgc gcggtgatga tgccgtcgcg gccgaatgtg
      841 gtcgagtacc agggcacgcc cgcgtatgga tagtcgccgt gctccagccg ggtgttgagc
      901 attaccaggt ccgcggccga gcggtccagc cagcggttga acagcgggtc cgaggtcgaa
      961 accttcgagc gcaccaagag cgaagtaccg acatcctgat tgatcttctt gaacgcggag
     1021 aagtagtcga ggtcgaccgg cgcatgttca tcgtcgcgct cgcagccgac ggtaacgtac
     1081 acatgcgcct cgcccttggg cggcaggtcg atccggtatt cggcccgccc ggtggtaagc
     1141 gtatcgggct tggggtcgag ccggatgcgc gtcttgcgca cgcactggtc gcgcccccga
     1201 tagccaagca gcagctcgcg ttcgcccact tcgggctgaa gatactggcc ccgttccttg
     1261 cagtgatagc cgcgtacctc gaagatgtcc ttgtagtcgg ccgcgaactc catcgcgagc
     1321 gtgatgcaga cctgctccag cccgaagttc acgacgcgca cgtgctcgaa gcaggcgttg
     1381 ttccacaaca gcttcgcgcg gaagatgtgc atctcgccct tcatgatgcg gttcgagccc
     1441 tccggatgca ggtccggatt catcagctcg acgaccagaa ggccgttgtc gtcgcgcacg
     1501 gtcgagttga ggaacatcgg gcgcacgccc tcgatcagca gttcctggtg cgagagaaaa
     1561 caggtgtcgc cgaggtacag gccttcctcg ccgagcccga tggattggat gtcgccgtag
     1621 cggtcgaaca tgccgaacag gtcgttgtgc ttgagtacgc gccgacgctc gtcggcaggc
     1681 gaagcggtcg cgaggatgta ccacttttcg ccgacccgga gtttttcaac catgtcgtca
     1741 cctcgtgcgc ggcgcggctt cggagtgccg gtcctgcagc gcccgataga tctgcaaata
     1801 gttggccgcc atgcgctcgg cggtaaagcg gcgctcgaac gcatctcggc aggcgcgccg
     1861 gtccagcgcg tgcaccttgc atgccgcctc gaccgcctgc tcctggttct cgacgatgaa
     1921 gccggttacg ccgtcgtcca ggatctccgg caccgcgccg ttgcggtagg caatgaccgg
     1981 cgtgccgcag gccaaggctt cgatcagcac cagtccgaat ggttcgggcc aatcgatcgg
     2041 gctgagcagc gccagcgcgc cgccgagcag ttcgcgtttt tcccgctcgc cgatctcccc
     2101 aacgtagcgc acgaatggtc gatcgagcag cggccgcacc tccttttcga agtagtcggg
     2161 ccgcgtttcg tcgatcttcg cggccacata aagcggcgtg ccgcaatgct gcgcgatctc
     2221 gatcgcgcgg tcgacgcgct tctccggaga gaaacggcca atgaacagga agtagccgcc
     2281 gtcgccgggg ccataccggt actggtcgcc cggcaggccg tgatggacgg tgccgaccca
     2341 gcgcgcgtgg ggcagcggtg actgctgttg tcgggaaatg gaaacgaccg ggacgtcgtt
     2401 gaagtgatgg aacagcggag cgagttccgg aaggtccagg cgccagtgca gcgtggtgac
     2461 atgggccacg cctgtgcggc gcaccagcgg gaagtgcagc aggtcagtgt ggaaatgcag
     2521 cacatcgaac tgcgcgatgc gctccatcac catgtcgagt tcgacgcagt gtgcgggaag
     2581 ccagctcgcc caccccggct gcggacgccc gctcaccggc accggcgcga ccaatgtggc
     2641 cgcggtgtcg gagtcgccgg acgcgaacaa tgtgacctcg tgtccctggc gcaccagctc
     2701 ttcagtcaga tacgaaacca cccgctccgt gccgccgtaa tgcctgggcg gaacgctttc
     2761 gaacagcgga gcgatctggg caattttcat cgcgaaacgc cgctaacggc agggttcgat
     2821 agacaacgta gacaccaggg cggagcgcga atttcgcgca cgattccgcg attgccacca
     2881 atgcattgaa cttgcagagg gggcgtgtca tcttgcaggg ggagtgatgc ccgcaggagc
     2941 acaacgatga acgagacagc agtcttcgcg ccgccgcgcg aacagcatga gccggagcgg
     3001 ccggatttcc ccgcgccaga gccggtcacg ccaccgtcgg agccggtgct gccggaaacg
     3061 gaacccgtgg ttcccgagcc ggaaccgctg gagccggaac gtgaaccgat gcaatgaaag
     3121 cgcatccacc cggccgcaat cgtcccggga aggcctgttc agtcgtgcgt gaagtgacag
     3181 atgtactcga ccggttcgtc ggcctccatg tcgaaacgcg tctggccggg cacgcgaaag
     3241 cgctgcccgg cgtgatactc cttccactcg ccgtcgtcgc cgatgcgggc gcggcaatga
     3301 ccggaaacga tttccatgat ctccggaccg tcggtggtga aggtcaccgt ggcgggaagc
     3361 agcacgccga tggtcttgcg gctgccgccc gggaagtaga ggttgtgcga gatgcatttg
     3421 ccgccgaagt agatgttggc cttcttcgga atcgacacat gttcgaattg ggtgctcatc
     3481 gggcctcctg cgccggaacg gagaaacgtt ggatctgtcg cgggaatcca agcgtatgcc
     3541 tgtgcttcat caagagtttg agccagcgca gagaacggct gtcggagcgg gcagggcggc
     3601 cgcgcgctcc ggcaagggag cgatggtaaa agcctgcgga tcagaggcgc acgatcacgc
     3661 gccccttgac cttcccgtcg aggagttcgc gcgccatcgg aatcgcttgc tccagcgtga
     3721 tctcgctggc gatggcgccg atgatcctgc tgtcgacttc ccgggccagc gcctcccacg
     3781 cggccagccg caggtgcagg ggcggattga cgctgttaat cccgagcagc gacacgccgc
     3841 gcaggatgaa gggcgccacc gtcgccggaa agtccatgcc ctgcgccagc ccgcacgctg
     3901 ccaccgcgcc gcccagccgg gtgccggcgc agacgttggc cagcgtatgg ctgccgacgc
     3961 tgtcgatcgc gcccgcccag
//
LOCUS       DATLGF010000360         9761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_230446, whole genome shotgun sequence.
ACCESSION   DATLGF010000360 DATLGF010000000
VERSION     DATLGF010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 9761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9761
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230446"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            328..1500
                     /locus_tag="VM406_11075"
     CDS             328..1500
                     /locus_tag="VM406_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76547.1"
                     /translation="MALNLFSSKGTSLDKQRFTWRDMVGKPISKLDDDAFTRVRIILM
                     NGLELESLRFQHMAARMQGELRLPLAQIRRTEQHQATMVNWLLGPDHSPLETTIAYEQ
                     VAIEVTAAVAQREPDPYFAQLYRFGMLEDFDHLYRYSALLDRLEGKDANNILQSYTDI
                     LPGRPTVEEHRAPEDDVRRSYDPKTAEPLTKLHALMITAAEWQTHDYYMNIGPLFSDP
                     MARQLYAEIASIEEQHVTQYGSMLSPEESVLEQWLMHEAMEAYNYYSCVQAETNPRIK
                     AIWERFLDYELGHFHHVAALYKKHERRDPAELFPTDLPEPVAFESQRDFVRKVLKQEV
                     DLRAAGTEIVEKSKESQASIAYRNHLNSEGSPSETVAAGYIWSPGTELNRKVTVAV"
     gene            1520..2299
                     /locus_tag="VM406_11080"
     CDS             1520..2299
                     /locus_tag="VM406_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HVL76548.1"
                     /translation="MKEQSQMGMNRTGVQMSPLDTHDMQNALPESKRPPAPGEQTAMA
                     ALRTSYIEQADQVGSIPVPGTVKGALSTGAAMLTGNQPQLLIDKLGERAAFERGGTRL
                     YDALITKFEATEDNTTSMTLADLQKIRDDEARHFALIVSAIESIGGDPTAMTPCADVT
                     AVESMGLMQVVNDPRTTLAQCLHAMLVAEMTDEVGWEHLIVLAEDNGQSQMVNDFTVA
                     LNDEQQHLMQVRRWFEEATLGKAISSGVMVDDVADAGRPLH"
     gene            complement(2296..3180)
                     /locus_tag="VM406_11085"
     CDS             complement(2296..3180)
                     /locus_tag="VM406_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVL76549.1"
                     /translation="MHFDLTDLRLLVSVAESGSLSRAAASFPIALSAASNRLRHLEDR
                     VGLTLFVRHADGMTPTPAGRVALEHARRVLAETRQMREALESLGGKRRVAIRVAANTV
                     ANSAYLPPALGPFLRDFPEVDLQVEERSSRDVLRNVQAGEVDIGVLDGNLPLRDVVSL
                     PFRHDRMVLLAPQGHALAARGHCLLRDALGFPFVGMPVERAMQRFVEDMATALGRPLS
                     MRVRAPSFYAIAQLVAQDVGLAVLPEEAARRHAATVPVAIVVLDDSWATRELRLCVRS
                     LDGLNAQARQLVQYLTGV"
     gene            3255..4214
                     /locus_tag="VM406_11090"
     CDS             3255..4214
                     /locus_tag="VM406_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVL76550.1"
                     /translation="MQSPLTRLFHDGRFYAFLAAAGASMKAIFVKLAYAAGPVDPVTL
                     LAMRMGIALPFFIWMAWISGRKSSSALSGPDLMRIAALGFVGYYLSSLFDFYGLVHIT
                     AGLERMILYLYPTMVLLMQAWLVRRAPDRRAWSAMAICYAGLAIAFAHDLQQEQGGNV
                     VVGAAWVFACAVSYALYYVGTGRLVGRIGSTRLAGLAGSASALFVLAHFLLVGEPRAL
                     PDLPPDVWLHAGLMALLSTVLPVWWLALAIQRLGAAQAAGIGTLGPVMTVFAAWLLLG
                     ESLSVLQLCGLALVLFGVMRLKPSQPKAVSAPAPAASAAGRRP"
     gene            4222..4968
                     /locus_tag="VM406_11095"
     CDS             4222..4968
                     /locus_tag="VM406_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVL76551.1"
                     /translation="MDEAMVTLAAVVALVFVVAGFVKGVIGLGLPTVAVGLLGLVMSP
                     VQAVTLLVVPSLVTNLWQLAAGPALMPLVQRLWPMLLGICAGTAGAAAVGLHAASGSG
                     PLLGAALVAYALFGLANKSPALPARAEMWLAPLAGAATGAVTVATGVFVLPAVPYLQA
                     LQLGKDELVQALGLSFSVSTLAIAIVLAGDGGFHVSGVVASLLTLAPALAGMMLGQWV
                     RARVPAPIFRRCFFIALFLLGAHLALRPAF"
     gene            5039..5416
                     /locus_tag="VM406_11100"
     CDS             5039..5416
                     /locus_tag="VM406_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HVL76552.1"
                     /translation="MSDPVRLDKWLWAARFFKTRTLATDAVDGGKVRVNGERVKPARN
                     VKEGDTLDIDNGSTRWEVVVRAMADKRGSAAIAQALYAETEASMTRREMLAEERRVFR
                     DPGSTIKGRPTKRDRRLIDKSRE"
     gene            5587..6249
                     /locus_tag="VM406_11105"
     CDS             5587..6249
                     /locus_tag="VM406_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVL76553.1"
                     /translation="MRKGELTRAAILDVALDLASRDGLEGLTIGLLADRMNMSKSGVF
                     AHFGSREDLQIEVVKLYHQRFEQEVFYPSIKEPRGLPRLEAMFARWVKRVSVEIASGC
                     IYISGAVEYDDRPGAIREELASMVRAWQTALRRCVVQAVEEGHLPEETDPDQLVYEMY
                     GLILSLHHDARFLKISGSVDRAQIGFERLINSYRNPPLPCATPEAKASTQTAVKKTAS
                     SV"
     gene            6268..8058
                     /locus_tag="VM406_11110"
     CDS             6268..8058
                     /locus_tag="VM406_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HVL76554.1"
                     /translation="MGQYIAPLRDMQFVLHELLKVEDEFKNLPKHAEIDKDIINQVLE
                     EGGKFTSQVLFPLNHSGDREGCRLDRETHVVQTPAGFKEAYQQYVEAGWPALACDPEY
                     GGQGLPIVVNNCFYEMLNSANQAWTMYPGLSHGAYEAIHAHGTPEQKALYLPKLVSGE
                     WTGTMCLTEPHCGTDLGLLRTKAEPQSDGSYKITGGKIFISAGEHEMSKNIVHLVLAR
                     LPDAPSGTKGISLFIVPKYLPNADGSVGARNPIFCGAIEEKMGIHGNSTCQMNLDNAT
                     GWLIGEPNKGLNAMFVMMNAARLGVGMQSLGLTEVAYQNAVVYAKDRLQMRSLSGAKA
                     PDKPADPIIVHPDVRRMLLTARAYAEAGRAFTAYVALQIDRELNHPDEEVRKEAADEV
                     ALLTPIIKAFLTDNGWIATSECMQVFGGHGYISEWGMEQYVRDARINMIYEGTNTIQS
                     LDLLGRKILMDNGAKLRKFGEKIRAFVEENGTDEAMSEFVTPLGDIGEKVGKLTMEIG
                     MKAFQNQDEVGAAAVPYLRVAGHMVFAYFFAQMAKIALEKQDSGDDFYKAKLATARFY
                     FARLLPETAMLIRQARSGSASLMALEADLF"
     gene            8291..>9761
                     /locus_tag="VM406_11115"
     CDS             8291..>9761
                     /locus_tag="VM406_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HVL76555.1"
                     /translation="MSNFIVKKVAVLGAGVMGAQIAAHCVNAKVPVILFDLPAKEGPK
                     NGIVQRAIENLKKLNPAPLGDKDDAALIQAANYEDNLELLKGCDLIIEAIAERMDWKH
                     DLYKKVAPYIAPNAIFASNTSGLSITALSEGFDAELKARFCGVHFFNPPRYMHLVELI
                     PTVATRPEILDQLEGFLTTTLGKGVVRAKDTPNFIANRVGIFGMLATIHEAEKFGLSF
                     DVVDDLTGSKLGRAKSGTFRTADVVGLDTMGHVIKTMQDNLADDPFFGVYKTPEVLNK
                     LINAGALGQKSGAGFYKKAGKDILRFDPAKNDYVPSGAKADDIIGRILKEKDPAKRIK
                     ALHDSTNPQGQFLWAIFRDAFHYIAVHLESIADNARDIDFALRWGFGWKTGPFENWQA
                     AGWQQVAQWVKEDIEAGRALCNAPLPDWVFDGRTGVHTAEGSWSPAQKAYVPRSELPV
                     YQRQAFRAPLIGENTTDATKAGTTVFEDESVRIWHQNDDV"
BASE COUNT         1843 a         3128 c         3094 g         1696 t
ORIGIN      
        1 caagtgaacc ccgagcccac cccggctgac cggcaggcgc agcttgtctt gcgcggcaag
       61 gctggcgaaa ttcttcctct gctgttcgcg ccctgacccc tgcctgcacg gcaacacaga
      121 tgcgcggcgg caaagccgcg cgctttcatg ccgaccttgg cgatgtaggc attaccgctg
      181 cccgtgtaaa tgcgtacggt tgaaagcgcc cacgccgccg cgactgtcct gcaccagcca
      241 tcccgctctc ggcacgcctc ttgcaacgcc cgccagcccg ccggtggcga gcaccggcct
      301 ttcaagaaat caacaggagc aattgcgatg gccttgaatc tcttcagttc caaggggacg
      361 tccctggaca aacagcgctt cacatggcgc gacatggtcg gcaaaccgat cagcaagctc
      421 gatgacgacg cgttcacccg ggtgcggatc atcctgatga acgggctgga gctcgaatcg
      481 ctgcgcttcc agcacatggc ggcgcgcatg cagggcgagc tgcgcctgcc gctcgcccag
      541 atccggcgta ccgaacagca tcaggcgacg atggtgaact ggctgctcgg ccccgatcat
      601 tcgccgctgg aaacgacgat cgcctacgag caggtcgcca tcgaggtgac cgcggcggtg
      661 gcccagcgcg agcccgaccc gtactttgcc cagctgtatc gcttcggcat gctggaagac
      721 ttcgaccacc tgtaccgcta ttctgcgctg ctggaccggc ttgaaggcaa ggatgcgaac
      781 aacatcctgc agagctacac cgacattctc cccggccgcc cgaccgtgga agagcatcgc
      841 gcacccgagg atgacgtgcg ccgctcctat gatccgaaga ccgccgagcc gctgacgaag
      901 ctgcatgcgc tgatgatcac cgccgccgaa tggcagaccc atgactacta catgaacatc
      961 ggcccgctgt tctccgatcc catggcgcgt cagctgtatg cggagatcgc ctcgatcgag
     1021 gaacagcatg taacgcagta tggttccatg ctgagcccgg aagaatcggt gctcgagcag
     1081 tggctgatgc acgaggcgat ggaagcgtac aactactact cctgcgtgca ggcagaaacc
     1141 aatccgcgca tcaaggcgat ctgggaacgt ttcctcgact atgagctggg tcacttccac
     1201 catgtcgccg cgctgtacaa gaagcacgag cggcgcgacc cggccgaact gtttccgacc
     1261 gatctgcccg agccggttgc cttcgagagc cagcgcgatt tcgtccgcaa ggtgctcaag
     1321 caggaagtcg acctgcgcgc ggccggtacc gagatcgtcg aaaagagcaa ggaaagccag
     1381 gcgtcgattg cctatcgcaa tcacctgaat tcggaagggt cgcccagcga gaccgttgcc
     1441 gccggctaca tctggtcgcc cggtaccgaa ctcaaccgca aggtcaccgt cgcggtctag
     1501 tccataggaa ggaatgacga tgaaagaaca gagccagatg gggatgaatc gtaccggcgt
     1561 gcagatgtcg ccgctggaca cccacgacat gcagaacgcg ctgcccgaat ccaagcggcc
     1621 gccggcgccg ggtgagcaga ccgcgatggc ggcgctgcgc accagctata tcgaacaggc
     1681 ggaccaggtc ggttcgattc ctgtgcccgg cacagtcaag ggcgcactga gcacaggggc
     1741 ggcgatgctg accggcaacc agccgcagct gctgatcgac aagctgggtg agcgcgcggc
     1801 gttcgagcgc ggcggcaccc gcctgtacga tgcgctgatc accaagttcg aggccaccga
     1861 ggacaacacg accagcatga ccctggccga cctgcaaaag atccgcgacg acgaagcgcg
     1921 ccatttcgcg ctgatcgtca gcgccatcga atcaatcggc ggcgacccga ccgcgatgac
     1981 gccatgcgct gatgtgacgg cagtggaatc gatgggtttg atgcaggtgg tcaacgatcc
     2041 tcgcactacg ctcgcacagt gcctgcatgc gatgctggtg gcggaaatga cggacgaggt
     2101 cggctgggag catctgatcg tgctggccga ggacaatggt cagagccaga tggtgaacga
     2161 cttcacggtg gcgctcaacg acgagcagca gcatctgatg caggtgcggc gatggttcga
     2221 ggaggccacc ctcggcaagg cgatctccag cggcgtgatg gtcgacgacg tggccgacgc
     2281 cggccggccg ctgcactaga ccccggtcag gtactgcacc agctggcgtg cctgggcgtt
     2341 cagaccgtcc aggctgcgca cgcacaggcg cagttcccgg gtggcccagg aatcgtccag
     2401 caccacgatc gcaaccggta cggtcgctgc atggcgccgt gccgcctcct ccggcagaac
     2461 cgccagtccc acgtcctgcg cgacgagctg ggcaatcgcg tagaagcttg gtgcacgcac
     2521 gcgcatgctg agcgggcggc cgagcgccgt cgccatgtcc tcgacgaagc gctgcatggc
     2581 ccgctcgacc ggcatgccga cgaatggaaa gccgagcgca tcgcgcagca ggcaatgacc
     2641 gcgtgcggcc agcgcatggc cctgcggcgc gagcaggacc atgcggtcgt ggcggaacgg
     2701 cagggagacc acgtcgcgta acggcagatt gccgtcaaga acaccaatgt ccacctcgcc
     2761 tgcctgcaca ttgcgcagca cgtcgcggct cgaacgctcc tcgacttgca ggtcgacttc
     2821 cggaaagtcc cttagaaacg gcccgagcgc gggcggcagg taggcgctgt tggccacggt
     2881 gttggccgcc accctgatgg cgactcgccg cttgccgcca agactttcca gcgcctcgcg
     2941 catctggcgt gtttccgcca gcacccgacg tgcgtgctcg agagcaacac gtcccgccgg
     3001 cgtcggcgtc atgccgtccg cgtggcggac gaacaaggtc aagccgacgc gatcctccag
     3061 gtgacgcaga cggttgctgg cggccgacaa cgcgatcgga aagctcgctg ccgcccggct
     3121 caggcttcca gattcggcta ccgacaccag cagcctcaaa tcagtcaggt cgaagtgcaa
     3181 ggtttctccc acggagaagg aaagctgcgt gaaacaagga ttctagccgc gaaggctacg
     3241 cgcgagaata aggcatgcaa agtcctctca cccgtctttt ccacgacggc cgcttctacg
     3301 cgtttctcgc tgccgccggc gcgtcgatga aggccatctt cgtgaagctt gcctacgcgg
     3361 ccggccctgt cgacccggtc acgctgctgg ccatgcgcat gggaattgca ttgccattct
     3421 tcatatggat ggcatggatc agcggccgga agtcttcaag cgcgctcagc gggccggacc
     3481 tgatgcgcat cgccgccctg ggcttcgtcg gctattacct gtccagcctg ttcgatttct
     3541 acgggctggt gcatatcacg gccggcctcg agcgaatgat tctctacttg tatccgacca
     3601 tggtgctgct gatgcaggcg tggctggtgc gccgcgcgcc tgatcggcgc gcgtggagtg
     3661 ccatggcgat ctgctatgcc gggctagcga ttgcgttcgc gcacgatctg caacaggagc
     3721 agggcggcaa tgtggtcgtc ggtgccgcct gggtgttcgc ctgtgcggtg agctacgcgt
     3781 tgtactacgt cggcaccggc aggctggttg gccgcatcgg ctcgaccagg ctggcgggcc
     3841 tcgccggcag tgcctcggcg ctgttcgtgc tggctcactt cctactcgtc ggggaaccgc
     3901 gcgcattgcc ggacctgccg ccggacgtgt ggctgcatgc gggcctgatg gcgctgttgt
     3961 cgacggtgct ccctgtctgg tggttggcac ttgcgatcca gcgactcggc gccgcacagg
     4021 ctgcgggaat cggcacgcta gggccggtaa tgacggtgtt tgcagcttgg ctgcttctgg
     4081 gcgaatcgct gtcggtgctg caattgtgtg gactggccct cgtgctgttt ggcgtgatgc
     4141 ggctcaagcc gtcgcagcca aaggccgtat ccgcccccgc acccgccgcg tcggcagcgg
     4201 gccgccggcc ctgacggcgc catggacgaa gcaatggtca cgctggcagc ggtcgtcgca
     4261 ctggtcttcg tggtggccgg cttcgtcaag ggtgtgatcg gcctcggcct gcccaccgtg
     4321 gccgtcggcc tgctcgggct ggtgatgtcc ccggtgcagg cggtgacctt gctcgtcgtt
     4381 ccctcgctgg tcacgaatct ttggcagctc gccgccggac ccgcgctcat gccgctggtg
     4441 cagcgcctgt ggccgatgct gcttggcatc tgtgcaggaa ccgccggcgc cgcggcggtc
     4501 ggactgcatg cggcaagcgg atcggggccg ctgctcgggg cggcgctggt cgcctatgcg
     4561 ttgttcggcc tggccaacaa gtcgccggcg cttcctgccc gcgcggagat gtggctcgcc
     4621 ccgctggccg gtgcggcgac cggggccgtc acggtcgcca ccggtgtatt cgtgctgccc
     4681 gccgtcccct atctgcaggc actgcagctc gggaaggatg aactggtaca ggcgctcggc
     4741 ttgtcgttct cggtctccac gcttgccatt gccattgtgc tggccggcga tggcggcttt
     4801 cacgtttccg gtgtggtcgc gtccttgctc acgctggcgc cggcgctggc cgggatgatg
     4861 ctcggtcagt gggtgcgcgc ccgcgtaccg gcgccgattt tccggcgctg cttcttcatc
     4921 gcgctcttcc tcctcggcgc gcaccttgct ttgcgtcctg cattctgact gcggcatgcc
     4981 gcccaaggcg gacgcaggca tgctatagaa tgatccgatt ggcatcatac cgacagacat
     5041 gtccgatccg gtccgtctcg acaagtggct gtgggccgct cgttttttca aaacccgtac
     5101 cctggcaacc gacgccgtcg acggcggcaa ggtccgcgtc aacggcgaac gcgtcaagcc
     5161 cgcgcgcaac gtgaaggagg gggacaccct cgacatcgac aacggctcca cgcgctggga
     5221 agtcgtggtg cgtgcgatgg ccgacaagcg gggctcggcc gccattgccc aggcgctata
     5281 cgccgaaacc gaggccagca tgacccgccg cgagatgctc gccgaagagc gcagggtatt
     5341 tcgcgatccc ggtagcacaa tcaaagggcg tcccaccaag cgcgaccggc ggctcatcga
     5401 caagtcgcgc gagtgatgcg gacagccctg cggacactag aagaccccct ctaggatccg
     5461 gtttgacatt taaaatacgc tggataatag tacgagcgtt cgctttcgct gatccccgta
     5521 caagtcttgc atttttcgta gggaactgcc gtggccaaca acatgaccga ttcgacgacc
     5581 aaactgatgc gcaaaggcga acttactcgc gccgcgattc tcgacgtggc acttgatctt
     5641 gcaagccgtg acggcctcga agggctcacg atcggtctgc tcgcggatcg aatgaacatg
     5701 agcaagtccg gtgtgtttgc acacttcggt tcgcgcgagg acctgcagat cgaggtggtc
     5761 aagctgtatc accagcgctt cgagcaggaa gtgttctacc cgagcatcaa ggagccgcgc
     5821 gggctgccac gactggaagc gatgttcgcg cgctgggtca agcgcgtctc ggtcgagatc
     5881 gcctccggct gcatctacat cagcggcgcc gtcgaatacg acgaccgccc tggcgcgatt
     5941 cgcgaggagc tcgcgagcat ggtgcgcgct tggcagacgg cgctgcggcg ctgcgtcgtg
     6001 caggcggtgg aggaaggtca cctgcccgag gaaaccgatc cagatcaatt ggtctacgag
     6061 atgtacggtc taatcttgtc gctgcatcac gacgcgcgct tcctcaagat ttccggcagc
     6121 gtcgatcggg cgcaaatcgg cttcgaacgc ctgatcaaca gctatcgcaa tccgccgctc
     6181 ccttgcgcca ccccggaggc gaaggcgagc acccaaaccg ctgtcaagaa aacagcttca
     6241 tccgtatagt catcaggaga taaagacatg ggtcaataca tcgctcccct gcgcgacatg
     6301 cagttcgttc tgcatgagct gctgaaggtg gaagacgagt tcaagaatct gcccaagcac
     6361 gcggagatcg ataaggacat catcaaccag gtgctggaag agggcggcaa gttcacgtcc
     6421 caggttctgt tcccgctgaa ccactccgga gaccgcgagg gctgtcgcct ggacagggaa
     6481 acccatgtcg tccaaacgcc ggcgggcttc aaggaagcct accagcaata cgtggaagcc
     6541 ggctggcccg cgctggcctg cgatccggaa tacggcggcc agggcctgcc gatcgtggtc
     6601 aacaactgct tctacgagat gctgaactcg gccaaccagg cctggaccat gtatccgggc
     6661 ctttcgcacg gtgcctatga ggctatccat gcgcacggaa cgcctgagca gaaggcactg
     6721 tatctgccca agctggtttc gggcgaatgg accggcacga tgtgcctgac cgagccgcac
     6781 tgcggtaccg acctgggcct gttgcgcacc aaggctgagc cgcagtccga tggctcttac
     6841 aagatcaccg gcggcaagat cttcatctcg gcgggcgagc atgagatgtc gaaaaacatc
     6901 gtgcacctgg tgctcgcgcg cctgccagat gcgccgtccg gcaccaaggg catctcgctg
     6961 ttcatcgtgc ccaagtacct gccgaacgcc gacggttccg tcggtgcgcg caacccgatc
     7021 ttctgcggtg cgatcgaaga gaagatgggc atccatggca actcgacctg ccagatgaac
     7081 ctggacaacg cgaccggctg gctgatcggc gaaccgaaca agggcctgaa tgcgatgttc
     7141 gtgatgatga atgccgctcg cctgggcgtt ggcatgcaat cgctcggcct gactgaagtc
     7201 gcctaccaga atgcggtcgt ctatgcgaag gaccgcctgc agatgcgcag cctgtccggc
     7261 gcgaaggcac cggacaagcc ggccgatccg atcatcgtgc atccggatgt gcgccgcatg
     7321 ctgctgaccg cacgcgccta cgccgaagcg ggtcgcgcat tcaccgccta tgtcgcgctg
     7381 cagatcgacc gcgaactgaa ccacccggac gaggaagtgc gcaaggaagc cgccgacgaa
     7441 gtggcgctct tgaccccgat catcaaggcc ttcctgaccg acaacggctg gatcgcgact
     7501 tccgagtgca tgcaggtgtt cggcggccac ggctacatct ccgagtgggg catggagcag
     7561 tatgtgcgcg acgcgcgcat caacatgatc tacgaaggca ccaacaccat ccagtcgctc
     7621 gatctgctcg gccgcaagat cctgatggac aacggcgcca agctgcgcaa gttcggcgag
     7681 aagatccgcg cgttcgtcga agagaacggc acggatgagg cgatgtcgga attcgtcact
     7741 ccgctcggcg acatcggcga gaaggtcggc aagctgacga tggaaatcgg catgaaggcg
     7801 ttccagaacc aggatgaagt cggcgccgct gccgtgccct acctgcgcgt ggccggtcac
     7861 atggtgtttg cgtatttctt cgcacagatg gcgaagatcg cgctggagaa gcaggattcc
     7921 ggcgatgact tctacaaggc caagctggcg accgcgcgct tctacttcgc gcgcctgctg
     7981 ccggagacgg cgatgctgat tcgtcaggcg cgttcggggt ccgcgagctt gatggctctc
     8041 gaagcagact tgttctaagc attccactga agagctcctt tcccctctag ggggaaggtt
     8101 gggatggggg tgcaaaggtt gaatcagcgc aggcgctcgt tcgaccaact tgcccccacc
     8161 cacgcagtgc ggagtacggg cttgcaagcc cgtaccgttg cgccggcggg cgacatgtcg
     8221 cccgaaaccc cggctccacc cccctaaaag ggggggaacc agacaccaga agattaagag
     8281 gaatcctcca atgtccaact tcatcgtcaa gaaagtcgcc gtcctcggcg ccggcgtgat
     8341 gggcgcgcag atcgcggccc actgcgtgaa cgcgaaagtc cccgtcatcc tgttcgacct
     8401 gcctgcgaag gaaggtccga agaacgggat cgtgcagcgc gcgatcgaga acctgaagaa
     8461 gctcaatccc gcgccgctgg gcgacaagga tgacgccgcg ctgatccagg ccgccaacta
     8521 cgaagacaat ctcgaactgc tcaagggctg cgacctgatc atcgaggcga tcgccgagcg
     8581 catggactgg aagcatgacc tgtacaagaa ggtcgcaccg tacatcgcgc cgaacgcgat
     8641 cttcgcgtcc aacacctcgg gtctgtcgat caccgcgctg tccgaaggtt tcgatgccga
     8701 gctgaaggcg cgcttctgcg gcgtgcactt cttcaacccg ccgcgctaca tgcatctggt
     8761 cgaactgatc ccgaccgttg ccacgcgtcc cgagatcctc gaccagctcg aaggcttcct
     8821 gaccacgacg ctgggcaagg gcgtggtgcg cgcgaaagac acgccgaatt tcattgccaa
     8881 ccgcgtgggc atcttcggca tgctggccac catccatgaa gcggagaagt tcgggctctc
     8941 gttcgacgtg gtcgacgatc tgaccggttc caagctcggc cgcgcgaaat ccggcacctt
     9001 ccgcaccgcc gacgtggtcg gcctggacac gatgggccat gtgatcaaga ccatgcagga
     9061 caacctcgcc gatgatccgt tcttcggcgt ctacaagacc ccggaggtgc tgaacaagct
     9121 gatcaacgcg ggcgcgctcg gccagaagag cggggcgggc ttctacaaga aggccggcaa
     9181 ggacatcctg cgcttcgatc cggccaagaa cgactacgtg ccctcgggcg cgaaggcgga
     9241 cgacatcatc ggccgcatcc tgaaggaaaa ggatccggcc aagcgcatca aggcactgca
     9301 cgattccacc aatccgcagg gccagttcct gtgggcgatc ttccgcgacg ccttccatta
     9361 cattgcggtc cacctcgaat cgatcgccga caatgcgcgc gacatcgact tcgcgctgcg
     9421 ctggggcttc ggctggaaga ccggtccgtt cgagaactgg caggcggccg gctggcagca
     9481 ggtcgcgcag tgggtgaagg aggacatcga ggccggccgc gcgctgtgca atgccccgct
     9541 gcccgactgg gtattcgacg gccgcaccgg tgtgcatacc gccgaaggct cgtggtcccc
     9601 ggcgcaaaag gcgtatgtgc cgcgttccga actgccggtc tatcagcgcc aggctttccg
     9661 cgcgccgctg atcggcgaga acacgaccga cgcgaccaag gccggcacca cggtgttcga
     9721 ggacgagtcg gtgcgcatct ggcaccagaa cgatgatgtt c
//
LOCUS       DATLGF010000361         3183 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_140093, whole genome shotgun sequence.
ACCESSION   DATLGF010000361 DATLGF010000000
VERSION     DATLGF010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140093"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VM406_11120"
     CDS             complement(<1..241)
                     /locus_tag="VM406_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76556.1"
                     /translation="MPLPYARQLFLLCLAAAAAAPAWAQHAVPALQPLEDAGEPGTLI
                     EARPAPRDGTNEIRMPGGRVTEIEVRMLVSRAPPAG"
     gene            complement(316..1059)
                     /locus_tag="VM406_11125"
     CDS             complement(316..1059)
                     /locus_tag="VM406_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004530319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HVL76557.1"
                     /translation="MSSTGKIVPRLRQVADIERATAKKARESWHMLTIMAEFIESTER
                     LSEVRPAVSIFGSARIKPDNPYYAKCVDIARRLSDAGLAVISGGGPGIMEAANKGAFE
                     GASSSIGLNIELPHEQQANPWQNISISFRHFFARKVAFVKYADAYVVMPGGFGTLDEL
                     MEVLTLIQTRTTRRIPVILVGTAFWRGLLDWFREQLVGNGLIAPADMDLVHLIDEPAH
                     VVEAIFAFYEAGEDLKGPPPPESKSSAYL"
     gene            1060..>3183
                     /gene="polA"
                     /locus_tag="VM406_11130"
     CDS             1060..>3183
                     /gene="polA"
                     /locus_tag="VM406_11130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HVL76558.1"
                     /translation="MNNTLLLVDGSSYLYRAFHAMPDLRSPEGEPTGAIYGMINMLRR
                     LRSDYGAAYMACVFDAKGKTFRDDIYPEYKAHRPPMPEDLARQVEPIHQAVRALGWPI
                     LMVEGIEADDVIGTLAAKAVEHGMQAVISTGDKDMAQLVNEHVTLINTMTNEKLDRDA
                     VIAKFGVPPERIIDYLTLIGDTVDNVPGVEKVGPKTAVKWINQYGSLDGIIAQADSIG
                     GAVGANLKRALDWLPTGRTLITIKTDCELVEHMVSITESLVAREPDNEALAAFYRRFG
                     FKTWLRELTGEIVQPRAVQPNETQQTALFEAPATKEYETVLSAERLEQWIATIMAAPL
                     TAFDTETTSLDPMRAELVGISLSCKPGVAAYIPVAHRYPGAPEQLSRDFVLEKLKPWL
                     EDASRPKLGQNLKYDCHVLANHGVALRGIAHDTLLQSYVFESHKSHDMDSLALRHLGR
                     KTITYEEVCGKGAGQLCFDAVAIERATEYAAEDADITLQLHQVMLPQVEADEGMKHVY
                     ERIEVPTSVVLQKIERNGVLIDTELLQRQSHELGARMLEMEKQAHELAGQPFNLNSPK
                     QICEIFFDKLALPVVKKTPSGTPSTDEDVLQKLAENYPLPKILLDYRGVSKLKSTYTD
                     KLPKMVNPGTGRVHTNYAQAVAVTGRLASNDPNLQNIPVRTAEGRRIREAFIAPPGSV
                     IVSADYSQIELRIMAHISGDASLLRA"
BASE COUNT          628 a          992 c         1042 g          521 t
ORIGIN      
        1 cacccgctgg cggcgcacgc gataccagca tgcgcacctc gatttcggta acgcgtccgc
       61 cgggcatgcg gatctcgttg gtgccgtcgc gcggtgccgg acgcgcctcg atcagcgtac
      121 ccggctcgcc cgcgtcttca agcggctgca gcgccgggac ggcgtgctgg gcccatgcgg
      181 gcgccgcggc ggcagcggca aggcagagca ggaacaactg gcgggcgtag ggcaggggca
      241 tggcgtgagg tgcgggcgcg gaatcggcaa atgcagtgcc cggcatttta gcaaaccgat
      301 cctgcgcgca agcaatcaaa gataggcgct gctcttgctt tccggcggag gcggtccctt
      361 caagtcctcg ccggcttcat agaatgcaaa aatcgcctcc accacgtggg ccggttcgtc
      421 gatcagatgc acgagatcca tgtcggccgg agcgatcagg ccattgccga ccagctgttc
      481 gcggaaccag tcgagcaggc cgcgccagaa cgcggtgccg accaggatga ccgggatgcg
      541 gcgggtggtg cgggtctgga tcagggtaag cacttccatc agttcgtcga gcgtgccgaa
      601 gccgcccggc atgaccacgt atgcgtccgc gtatttcacg aatgcgacct tgcgggcgaa
      661 aaaatggcgg aagctgatcg aaatgttctg ccacgggttg gcctgctgtt cgtgcggcag
      721 ttcgatattc aggccgatcg atgaggacgc gccttcgaac gcacccttgt tcgcggcctc
      781 catgatgccg ggacccccgc ccgagatcac ggccaggccg gcatcggaca ggcgacgcgc
      841 gatatcgacg catttcgcgt agtacgggtt gtccggcttg atgcgggccg aaccgaagat
      901 cgagactgcc ggacgcacct cggacaggcg ttcggtcgac tcgataaact ctgccataat
      961 cgtgagcata tgccacgact cgcgcgcctt cttcgccgtg gcccgctcga tatccgccac
     1021 ctgccgcaga cgcggcacga tttttcctgt tgacgacata tgaataacac cctgctgttg
     1081 gttgatggtt ccagctattt gtatcgcgcc ttccacgcga tgccggacct gcgcagtccg
     1141 gaaggagaac ccaccggcgc gatctacggg atgatcaaca tgctgcgcag gctgcgcagc
     1201 gattacgggg cagcatacat ggcctgtgtg ttcgacgcaa aagggaagac gtttcgcgac
     1261 gacatctatc cggagtacaa ggcgcacagg ccgccgatgc cggaagacct ggcgcgccag
     1321 gtcgagccga tccaccaggc ggtgcgcgcg ctcggctggc cgatcctgat ggtcgagggc
     1381 atcgaggcgg acgacgtgat cggcacgctc gccgcgaagg cggtcgagca cggcatgcag
     1441 gcagtgattt cgaccggcga caaggacatg gcccagctgg tcaacgagca cgtcacgttg
     1501 atcaatacca tgaccaacga gaagctcgac cgcgacgcgg tgatcgcgaa gttcggcgtg
     1561 ccgcccgagc gcatcatcga ttacctgacc ctgatcggcg acaccgtcga caacgtgccc
     1621 ggcgtggaaa aggtcggtcc caagacagcg gtcaagtgga tcaaccagta tggttcgctg
     1681 gacgggatca tcgcccaggc ggacagcatc ggcggcgcgg tgggcgcgaa cctcaagcgc
     1741 gcgctcgact ggttgccgac ggggcgcacg ctcatcacga tcaagaccga ctgcgagctg
     1801 gtcgagcaca tggtgtcgat caccgaatcg ctggtggcgc gcgagccgga caacgaggcg
     1861 ctggccgcgt tctaccgccg cttcggcttc aagacctggc tgcgcgagct gaccggagag
     1921 atcgtgcagc cacgcgcggt gcaaccgaac gaaacgcagc agaccgcctt gttcgaggcg
     1981 ccggccacga aggagtacga aaccgtgctc agcgcggaac ggctggagca gtggatcgcg
     2041 accatcatgg ccgcaccgct cacagccttt gataccgaga cgacgtctct ggatccgatg
     2101 cgcgccgaac tggtcggcat ttcactgtcc tgcaagcccg gcgtcgcggc ctatattccg
     2161 gttgcgcacc gctaccccgg cgcaccggag cagctctcgc gcgacttcgt gctcgagaag
     2221 ctgaaaccgt ggctggagga tgcgtcgcgg cccaagctcg gccagaacct gaagtacgac
     2281 tgtcacgtgc tcgccaacca cggcgtcgcg ctgcgcggca ttgcgcatga cacgctgctg
     2341 cagtcctatg tgttcgaatc gcacaagagc cacgacatgg acagccttgc gctgcgccat
     2401 ctcggccgca agaccatcac ttacgaggaa gtgtgcggaa aaggcgcggg acagctgtgc
     2461 ttcgacgcgg tggcgatcga gcgggcgacc gagtacgcgg ccgaggacgc ggacatcacg
     2521 ctgcagctgc accaggtgat gctgccgcag gtcgaggccg acgagggcat gaagcatgtg
     2581 tacgaacgca tcgaggtgcc gacctcggtg gtgctgcaga agatcgagcg caacggcgtg
     2641 ctgattgata cggagctgct gcagcgtcaa tcgcatgagc tcggcgcgcg catgctggag
     2701 atggaaaagc aggcgcatga acttgccggc cagccgttca acctgaattc gccgaagcag
     2761 atctgcgaga tcttcttcga caagctggcg ctgccggtgg tcaagaagac gccgtccggc
     2821 actccgtcga ccgacgagga cgtgctgcag aagctggcag aaaactaccc gctgccgaag
     2881 atactgctcg actaccgcgg cgtgtctaag ctgaagtcca cttataccga caagctgccg
     2941 aagatggtca atccgggtac cgggcgcgtg cacaccaatt atgcgcaggc ggtggcggtg
     3001 accggacggc tcgcatcgaa cgatccgaac ctgcagaaca tcccggtgcg caccgccgag
     3061 ggacgacgca tccgcgaggc cttcattgcg ccgccgggaa gcgtaatcgt ttcggccgac
     3121 tattcgcaga tcgagctgcg catcatggcg cacatctcgg gcgatgcgag cctgcttcgt
     3181 gca
//
LOCUS       DATLGF010000362         3056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_182461, whole genome shotgun sequence.
ACCESSION   DATLGF010000362 DATLGF010000000
VERSION     DATLGF010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182461"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VM406_11135"
     CDS             <1..326
                     /locus_tag="VM406_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HVL76559.1"
                     /translation="PKELGGPGGDGTNPEQLFAAGYSACFLSALKFVAMHAKTALPAD
                     ASVSGSVAIGPNDKGGFGLSAKLRVSLPGFEQELAQALVDKAHAVCPYSNATRGNINV
                     EVVLV"
     gene            445..867
                     /locus_tag="VM406_11140"
     CDS             445..867
                     /locus_tag="VM406_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVL76560.1"
                     /translation="MAAIPPFHLAFPVHDLDAARHFYGEVLGCAEGRSSPDWIDFDFY
                     GHQIVTHFVGAKTVHAGQGHVDAKQVPIPHFGVVLTMEDWRALADRLEKSGIEFVMRP
                     QIRFAGQPGEQATMFFLDPSNNAIEVKGFADMSKLFAK"
     gene            876..1817
                     /locus_tag="VM406_11145"
     CDS             876..1817
                     /locus_tag="VM406_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HVL76561.1"
                     /translation="MRILFHMPSGDPAPWLADLARELPQAQVRVWQTGDDAPADYALV
                     WKPPPELLRGRAGLKAVFNLGAGVDAILQLLRDEPGLLAPQVPVIKLDDAGMGVQMVE
                     YVTHAVVRRFRRFDEYEDLQRRHAWHALAIPDRTSYRIGVMGLGALGARVATALAQYG
                     FPVRGWSNSRKTLDGVSAFAGDAELPGFLDRLNVLVDMLPLTRETTGILNSANLARLA
                     RGAHLINVARGAHVIEADLLEALDAGQVGWATLDVFRDEPLPADHPFWNSPRITLTPH
                     VSALTEREPSVRQIADRIRSIERGEQVAGVIDVKRGY"
     gene            1923..2708
                     /locus_tag="VM406_11150"
     CDS             1923..2708
                     /locus_tag="VM406_11150"
                     /inference="COORDINATES: protein motif:HMM:NF022665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HVL76562.1"
                     /translation="MSRTAAIITTLVILTGLIVGLLFWQERTRFEPQITPRSEAPPDD
                     AIDEPAIRYPVPAAEAPAEPLPQLDESDPSFESALSSLVGPRTFRALYQPQSLVRRIV
                     VTVDNLPREVVADRLRPIQPAQGRFLVQHEGNTVRIAPDNAKRYTPYVRALEAVNVER
                     FTQVYARFYPLFQDAYRDLGYPNAYFNDRLVAVIDHMLAAPELSGPPQLVQPHVFYRY
                     ADPALESASAGHKLMWRIGNENARRVKAKLREIRTAVTAHKPE"
     gene            2948..>3056
                     /locus_tag="VM406_11155"
     CDS             2948..>3056
                     /locus_tag="VM406_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011544732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HVL76563.1"
                     /translation="MNLRPLHDRVIVKRLDQETKTASGIVLPEAAAEKPD"
BASE COUNT          563 a         1024 c          935 g          534 t
ORIGIN      
        1 caccgaagga actgggcggc cccggcggcg acggcacgaa tccggagcaa ctgttcgcgg
       61 ccggctattc cgcctgcttc ctgtcggcgc tgaagttcgt tgccatgcat gccaagactg
      121 ccctgcccgc cgacgcatcg gtgtccggca gtgttgccat cggccccaac gacaagggcg
      181 ggttcggcct gagcgcgaag ctgcgcgttt cactgcccgg tttcgagcag gaactcgccc
      241 aggcactggt cgacaaggcg catgcggtgt gtccatactc gaacgccacg cgcggcaata
      301 taaacgtgga agtcgtgctc gtctgagcgg attcccggca ccctgccggt gccgggacgg
      361 gtgcgcaaaa agcaggaagg cgcgtctggc cttacactgt aggcgcgccg ctcccacgcg
      421 gcagaacatg aaacaaggac tcacatggct gccattcccc cctttcatct cgcctttccc
      481 gttcacgatc tcgacgccgc ccgccacttc tacggcgaag tgctcggctg tgcggaaggc
      541 cgcagttcgc ctgactggat cgacttcgat ttctacggcc atcagatcgt cacccatttc
      601 gtcggcgcca agaccgtgca cgcgggccag ggccacgtcg acgccaagca ggtaccgatt
      661 ccgcatttcg gcgtggtact gacaatggaa gactggcgcg cactggccga ccggctggaa
      721 aaatccggca tcgaattcgt gatgcgcccg cagatccgct ttgccggcca gcccggcgag
      781 caggcgacga tgttcttcct cgatccaagc aacaatgcca tcgaggtcaa gggcttcgcc
      841 gacatgagca agctgttcgc gaaataaggc ggcacgtgcg catcctcttc cacatgccgt
      901 ccggcgatcc ggccccgtgg ctggccgatc tcgcgcgcga actgccgcaa gcgcaggtac
      961 gcgtctggca gacgggagac gatgcgccag ccgactatgc gctggtctgg aaacctccgc
     1021 cggaactcct gcgcggacgt gcgggcctga aggccgtctt caatctcggc gcaggcgtcg
     1081 acgcgatcct ccagctgctg cgcgacgagc ccggcctgct tgccccgcag gtaccggtca
     1141 tcaagctcga tgacgccggc atgggcgtgc agatggtgga atacgtgacg catgcggtgg
     1201 tgcggcgctt ccgccgattc gacgaatacg aggacctgca gcgccggcac gcctggcatg
     1261 ccctggcgat tccggaccga acttcctacc ggatcggcgt gatgggcctg ggcgcgctcg
     1321 gcgcgcgtgt ggctaccgca ctggcgcagt acggctttcc ggtgcgcgga tggagcaaca
     1381 gccgcaagac gctggacggc gtcagcgcct tcgcgggaga tgccgaactg cccggcttcc
     1441 tggatagatt gaacgtgctg gtcgacatgc tgccgctgac ccgggaaacc acgggcatcc
     1501 tgaatagcgc gaaccttgcc cggctggcgc gcggagcgca cctgatcaat gtggcgcgcg
     1561 gcgcgcatgt gatcgaagcg gatctgctgg aagcgctgga tgcggggcag gtcggatggg
     1621 ccacgctgga tgtgtttcgc gacgagccgc tgccggcaga ccacccgttc tggaattccc
     1681 cgcgtatcac gctcacgccg catgtttccg cgctgacaga acgggagccg agcgtgcgcc
     1741 agattgcgga ccggatccgt tcaatcgagc gcggggagca ggtcgccggc gtcattgatg
     1801 tgaagcgcgg gtattgaccc gcttatattg acgcgcgctt gatgccgctc cacacccgaa
     1861 tgtgagcggg tggcatagtc gcacagctac ccctgcgcaa catgccttgc gggagagatc
     1921 cgatgagccg aaccgccgca ataattacca cgctggtcat cctgaccggc ttgatcgtcg
     1981 gcctgttgtt ctggcaggag cgcacccgct tcgagccaca gatcacgccg cggtctgagg
     2041 cgccgccgga cgatgcgatt gatgaaccgg cgatccgcta tccagtgccc gcagccgaag
     2101 caccggccga gcccctgccc cagctcgacg agagcgaccc gagcttcgaa tccgccctga
     2161 gcagcctggt aggcccgcgc accttccgcg cgctgtatca gccgcagagc cttgtgcgcc
     2221 gcatcgtcgt caccgtcgac aacctgccgc gcgaagtcgt cgcggaccgc ctgcggccga
     2281 tccagcccgc gcagggacgc ttcctcgtgc agcacgaggg caacacggtc aggattgcgc
     2341 ccgacaacgc caagcgctac acgccctacg tgcgcgccct ggaagcggtc aacgtcgaac
     2401 gttttaccca ggtctatgcg cgcttctacc cgttattcca ggatgcctac cgcgacctcg
     2461 gctatccgaa tgcgtatttc aacgaccgcc tcgtcgcggt gatcgaccac atgctcgccg
     2521 cgccggagct cagtggtccg ccgcagctgg tgcagccgca tgtgttctac cgctacgccg
     2581 atccggcact ggaatcggcg tctgccgggc acaagctcat gtggcgcatc ggcaatgaaa
     2641 acgcgcgccg cgtgaaggcc aagctgcgcg agatacggac ggccgtaacg gcacacaagc
     2701 ccgaatagcc tgtttgcgca ggatcaaccc cctccgcggc tccttcgcag accatcgcgg
     2761 ttcaccgatt cttcccgcct tgaaaaaata aaaccccacc catatactta gcactcgccg
     2821 gggtagagtg ctaacaactt ctcttcggac cactttcccg agcggcagga ctggcgccgt
     2881 gaaaccgctt ttgctgcacc tccctgctgc aaaataaaac ataacctatt gattttaagg
     2941 agtttgtatg aaccttcgtc ctttgcacga tcgcgtcatc gtcaagcgcc tcgaccagga
     3001 aaccaagact gcgtccggca tcgttctccc ggaagcggct gcggaaaagc ctgacc
//
LOCUS       DATLGF010000363         2336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_213616, whole genome shotgun sequence.
ACCESSION   DATLGF010000363 DATLGF010000000
VERSION     DATLGF010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213616"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VM406_11160"
     CDS             complement(<1..268)
                     /locus_tag="VM406_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HVL76564.1"
                     /translation="MRSIRRTLLLWLYAGLSLGIAAAAAVLYFQARAETNRILDFQMQ
                     QLAWSLPGQPFAPITPERRTLPALPEGAILIQIWDRNGVRIYYSH"
     gene            complement(265..921)
                     /locus_tag="VM406_11165"
     CDS             complement(265..921)
                     /locus_tag="VM406_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL76565.1"
                     /translation="MRLLLVEDDPMIGAGVRRGLQQDGFVVDWLRDGRSAESAFDPSL
                     HELVLLDLGLPGRDGIEVLTKLRRRGETVPVLIMTARDAVADRVAGLDAGADDYLVKP
                     FDLEELAARIRALLRRRAGRAEMVLQHGPLTLDPARHEATLDGVPLALSAREFALLQA
                     LLERPGVVMSVAALQDKLYGWDDEVGSNTIEVYIHALRKKLGPGFIKNVRGVGYMIPE
                     "
     gene            complement(943..1716)
                     /locus_tag="VM406_11170"
     CDS             complement(943..1716)
                     /locus_tag="VM406_11170"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HVL76566.1"
                     /translation="MLHSARNLKGFAVVATDGSIGKIDDVYFDDEKWVIRYVVVDTGG
                     WLTGRKVLLSPMSFRDADWQGRKMMVNLTRDRVRNSPGIDTHKPVSRRQEGALYEYYG
                     YPYYWSGPYAWGYAAMPAVTEQEVFDSPERQHTREAMEQTSDEDMHLRSCADVTGYAI
                     HATDDTLGHVDDFLFDEQDWSIRLVLVDPRDWWPNKDVMVPPERIVHVNWEDGTVDVN
                     MSRAEIESSPEYDPDHPPPSTLQEARAHRPHEGDQQPRL"
     gene            complement(1808..2014)
                     /locus_tag="VM406_11175"
     CDS             complement(1808..2014)
                     /locus_tag="VM406_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76567.1"
                     /translation="MPDEKEGIDKSGGDYLPADEAARLTGAEQERRSREGRAAAPHRI
                     QQVPREQMERSVPPPRDPDDPLSR"
BASE COUNT          367 a          807 c          743 g          419 t
ORIGIN      
        1 cgtgggagta gtagatgcgc accccgttac ggtcccagat ctgaatcagg atcgcgccct
       61 ccggcagcgc cggcagcgtg cgccgctccg gcgtgatcgg tgcgaacggc tggccgggca
      121 aggaccaggc gagctgttgc atctggaaat cgaggatgcg attggtttcg gcgcgtgcct
      181 ggaagtaaag cactgccgcg gcggcggcaa tgccgagcga caggcccgca tacagccaga
      241 gcagcagcgt gcggcggatc gacctcattc cgggatcatg tacccgacgc cgcgcacgtt
      301 cttgatgaag ccgggcccga gcttcttgcg cagcgcatgg atatagacct cgatggtatt
      361 gctgccgacc tcgtcatccc agccgtacag cttgtcctgc agcgcggcca ccgacatcac
      421 cacaccgggg cgttcgagca gcgcctgcaa cagcgcgaac tcgcgcgccg acagcgccag
      481 cggcacgccg tcgagcgtgg cctcgtggcg cgccggatcc agcgtcagcg gaccgtgctg
      541 gagcaccatc tcggcacgtc cggcacgccg gcgcagcaag gcgcggatgc gcgcggccag
      601 ttcttcgagg tcgaagggct tgacgaggta gtcgtcggcg ccggcatcga gaccggccac
      661 gcggtcggcc accgcgtcgc gcgcggtcat gatcagcacg ggcacggtct cgccccgcct
      721 gcgcaatttc gtcaggacct cgataccgtc ccttcccggc agcccgaggt cgagcaggac
      781 cagttcgtgc agcgaaggat cgaacgcgga ttcggcgctg cgcccgtcgc gcagccagtc
      841 gaccacgaag ccgtcctgct gcagcccgcg gcgcacgccc gcgccgatca tcgggtcgtc
      901 ctcgaccagc agcaggcgca tggcgcgttt ttacggctca attcacagcc tgggctgctg
      961 gtcgccttcg tgcgggcgat gcgcgcgcgc ttcctgcagc gtagagggcg gcgggtggtc
     1021 cgggtcgtac tccgggctgc tttcgatttc ggcgcgcgac atgttcacgt cgaccgtgcc
     1081 gtcctcccag ttcacgtgga cgatgcgttc cggcggcacc atcacgtcct tgttcggcca
     1141 ccagtcgcgc gggtcgacca ataccagcct gatcgaccag tcctgttcgt cgaacaggaa
     1201 atcatcgaca tggccgagcg tgtcgtcggt cgcgtggatc gcatagccgg tgacgtctgc
     1261 gcagctgcgc agatgcatgt cctcgtcact ggtctgttcc atggcctcgc gcgtgtgctg
     1321 gcgctccggg gaatcgaaca cctcctgctc ggtgacagcc ggcatcgccg catagcccca
     1381 ggcatagggg ccgctccagt agtacggata gccgtagtac tcgtagagcg cgccttcctg
     1441 gcgccgcgac accggcttgt gcgtatcgat gccgggactg ttccggaccc tgtcccgggt
     1501 caggttgacc atcatcttgc gcccctgcca gtccgcgtcg cgaaacgaca tcggtgacag
     1561 caggaccttg cggccggtga gccagccgcc ggtgtcgacg acgacgtagc ggatcaccca
     1621 cttctcgtcg tcgaaataca cgtcgtcgat cttgccgatg ctgccgtccg tggcgaccac
     1681 ggcaaaaccc ttcagattcc ttgcgctgtg tagcatcgca gcctcctggt tcgtgatgcc
     1741 ggcatgcacg gcgcgccgca tcgggaaatc tcccgccgct ccgctgcgcg gccgcatgcc
     1801 ggaccgctca tcgggagagc ggatcgtcgg gatcccgtgg cggcggcacg gaacgctcca
     1861 tctgttcgcg cggcacctgt tggatgcgat gcggcgccgc cgcacgcccc tcccggctgc
     1921 ggcgctcctg ttctgcgccc gtcagcctcg ccgcctcgtc cgccggcagg taatccccgc
     1981 cgctcttgtc gattccttcc ttctcgtcag gcatggtgcc ctcctcgtgt aactgaagat
     2041 ttgggccccc catctcccgg cgagttccgc gcaaagccgt ggagtaatta ccggcgcacg
     2101 gtaattcttt cccctcgcgg cgcgcacggt ccctgtcccg cctgcgcatc cgcaatggca
     2161 catcccttgc aaaaccattg ttcttttccg gaaccgtccc ataccgggga cgcgcacgca
     2221 caaggagaga acgtcatggc agacaccgtc ggagatttcc tgctgaaacg attgcatgaa
     2281 tggggcattc gccgcgtgtt cggctacccg ggtgacggca tcaacggcat catggg
//
LOCUS       DATLGF010000364         5393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_47279,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000364 DATLGF010000000
VERSION     DATLGF010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47279"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(286..894)
                     /locus_tag="VM406_11180"
     CDS             complement(286..894)
                     /locus_tag="VM406_11180"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL76568.1"
                     /translation="MNMNISPRAAGVRGFTSRAVSVILLAVLPMVSVSALGDTPRLDV
                     PYVPTPQPVVDRMLELAQVTDKDVVYDLGSGDGRIVVTAAREYGARGVGIDLNPQRVA
                     EARANAKEAGVQDRVEFRVGDLFQTDLSEATVVTLYLLTSVNYKLRPQLWRQLRPGTR
                     VVSHAFDMGDEWPPEKVERVGGSTIYYWTITEDHKRRVAAGN"
     gene            1151..1363
                     /locus_tag="VM406_11185"
     CDS             1151..1363
                     /locus_tag="VM406_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76569.1"
                     /translation="MHAYIVRTREGYLYPFAGDVSLTSDPTQAGHFLSTDEACEVAAS
                     RGYYDGHFDVLRVEIDESHLNRGWSQ"
     gene            1460..2101
                     /locus_tag="VM406_11190"
     CDS             1460..2101
                     /locus_tag="VM406_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HVL76570.1"
                     /translation="MSFSTWITFVFASALIAVSPGSGAVLAMSHGLSYGVRKASATIL
                     GLQGGLLLLTFIAGAGIGSVLLASEFAFSVVKVVGALYLIWLGFTQWRSPAAAVSAGP
                     MGNGTVVPSLRKRVLTGFLTNATNPKGIVFAVAMLPQFISPHAPLLPQLLILGATSVA
                     IDLVVMHGYAFAASSMQHWLASARAARSQNRFFGGVLMAVGATLFFVKRNPAA"
     gene            2153..2380
                     /locus_tag="VM406_11195"
     CDS             2153..2380
                     /locus_tag="VM406_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76571.1"
                     /translation="MSAEAFTFWQGLGVLFFWLVFLIPLWRIISKAGYSGAWALLSLI
                     PLVNILALWIFAFARWPSERERREDHGYPHR"
     gene            2542..2856
                     /locus_tag="VM406_11200"
     CDS             2542..2856
                     /locus_tag="VM406_11200"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HVL76572.1"
                     /translation="MFMTNIETVPGKTITAVHGVVTGSTVRAKHVGRDIMASLKNIVG
                     GELKGYTELLEDARRDAIERMQSQARQIGANAIVNVRFSTSAVAAGAAEIYVYGTAVT
                     VE"
     gene            2863..3315
                     /locus_tag="VM406_11205"
     CDS             2863..3315
                     /locus_tag="VM406_11205"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HVL76573.1"
                     /translation="MEAIVLWLLLLVAGYVFGQRAERRHYRSIEARERALLALPATNA
                     RTPVDPPGPVARAELVHGSVVISVDFFKRIAAGLRGIVGGRVQSYETLVDRARREAVL
                     RLKESCPGAGQVINLRLETATISGGGGRALGSVEVLAYGTALYFDTRE"
     gene            3312..4127
                     /locus_tag="VM406_11210"
     CDS             3312..4127
                     /locus_tag="VM406_11210"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HVL76574.1"
                     /translation="MKYKPALPEQNDNVSHRKPVREFFLILAGLSIAALALFWVLGLA
                     VDLAVDHMSAQAEARIHRALRVSYPQHKPHAPQMQARLERMVDELRACADLPYPVTLQ
                     LVKAEQPNAGVLPGAHILVFSGLLDKIETENALAFVLAHELAHLKNRDHLRGLGRTLL
                     LLAISITLTGTHSDLTQILVPANQLGMARHSQARETAADNTALDILHCHYGHVGGATE
                     FFEALHRRAGEAPVGLGHYFASHPELRARIDNLNRRAEQHAYPIGPTRALARQ"
     gene            complement(4174..4890)
                     /locus_tag="VM406_11215"
     CDS             complement(4174..4890)
                     /locus_tag="VM406_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HVL76575.1"
                     /translation="MKPLLIIKVGDALPVARERGHDFEHWIAAGFRIAASKVRIVDAR
                     RACDLPDPGLLAGAAVTGSAAMVTDREPWSESLVPWLRHAVEARLPILGICYGHQLLA
                     HALGGEVAYHPRGMEIGTVQIRKTAAAVDDPLFASLPRIFPAQAVHSQTVLGLPAGAV
                     RLAENEHDAHHAFRFGACAWGVQFHPEFDAEAMHGYIDKFATDLAARGRSPEDLRAQV
                     GMTAQAASILQRFARLALDA"
     gene            5144..>5393
                     /locus_tag="VM406_11220"
     CDS             5144..>5393
                     /locus_tag="VM406_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76576.1"
                     /translation="MTASTVFDHSPWVGRYKNIDWQLKTPPAASLPAMVSAASRAYAK
                     QKAFTCVMPNGMYGTLTYEQTDRMSDDFAVYLREVLKLA"
BASE COUNT          934 a         1827 c         1723 g          909 t
ORIGIN      
        1 acaggtcctc acgcgccgcg gccggcgcgg cgggcttttc aggcggcgca agcgaaactg
       61 tcggcagcgc cggtgtctcg ggacgtgaag gtgcgccgat caggcgctga aaccagccaa
      121 acatgggtca tccgcaggaa gcctgtatat gtggcaggca tcatagcgga agctgtttcc
      181 aattggaaac agctgcaccc atggattttt gccgatgccg ggaaagccag cggcgtagga
      241 aaagcggacc gagccccccg ggggccgccg gcttgcgctg tgacttcagt tgccggccgc
      301 gacgcgccgc ttgtggtctt cggtgatggt ccagtagtag atcgtgctgc cgccgacacg
      361 ctcgaccttc tccggcggcc actcgtcgcc catgtcgaac gcgtgcgaca ccacgcgcgt
      421 accgggccgc aactgccgcc atagctgcgg gcgcagcttg tagttcacgc tggtgagcag
      481 atagagggtg acaacggtgg cttcggacag gtcggtctgg aacaggtcgc cgacgcggaa
      541 ttcaacccgg tcctgcacgc ccgcctcctt cgcattggcg cgcgcctcgg ccacccgctg
      601 cgggttgaga tcgatgccca cgcccctggc gccgtactcg cgcgccgcgg tgaccacgat
      661 gcggccgtcg ccgctgccga ggtcgtacac cacgtccttg tccgtcacct gcgccagctc
      721 aagcatgcgg tcgaccaccg gctggggcgt cgggacatag ggcacatcga ggcgcggcgt
      781 gtcgccgagc gcggacacgc tgaccatcgg caatacggcg agcaagatga ccgagacggc
      841 gcgcgaggtg aaaccgcgga cgcctgccgc ccggggcgat atgttcatgt tcatgaaacg
      901 ctcctgtatc cggtgtggaa atgaatggtg gggtggaacg gacgatccgg ccccaacgcc
      961 agacgcatga aaaggacgat tggtttttcc ccgtccggca ctcatggact tcgctcaata
     1021 gggcgctacg atacctgctc gtggtgcgtg cggtgcagcg tcgcgcgcca ctcatgactt
     1081 tgcgcaagca cgatcagtgc ggtttacgct cgactatccg cacggcagac attccaggag
     1141 accactgatc atgcacgcct atattgtccg cacccgggaa ggctatctgt acccgttcgc
     1201 cggggacgtc tccctaacca gcgaccccac ccaggccgga cacttcctca gcaccgacga
     1261 ggcgtgtgaa gtggcggctt cgcgcggcta ctatgacgga cacttcgacg tgctgagagt
     1321 ggaaatcgac gaatcgcacc tgaaccgcgg gtggagccag taagccggca gggacagaca
     1381 ccagggcaga ggccaggggc cggcagcgcg gacgcgccct cgctataatc cgggctccct
     1441 cccggatcac cgcccgcgca tgagtttctc cacctggatc accttcgtct tcgcatccgc
     1501 gctgattgcg gtttcgcccg gctccggggc ggtgctggcc atgtcgcacg gcctttccta
     1561 cggcgtgcgc aaggccagcg cgacgatcct cggcctgcag ggcggcctgc tgctgctcac
     1621 cttcatcgca ggcgccggca tcggctcggt cctgctcgct tccgagttcg cattcagcgt
     1681 ggtcaaggtg gtgggcgcgc tctacctgat ctggctcggc ttcacgcagt ggcgcagccc
     1741 ggcagccgcg gtttccgcgg gtcccatggg aaacggcacc gtggtacctt cattgcgcaa
     1801 gcgggtgctg accggcttcc tcaccaacgc aacaaatccc aagggcatcg tgttcgcggt
     1861 cgccatgctg ccgcagttca tctcgccgca cgccccgctg ctgccccagc tcctgatcct
     1921 tggagcaacc tcggtggcga tcgacctcgt cgtgatgcac ggctacgcgt tcgccgcctc
     1981 ctcgatgcag cactggctcg ccagcgccag ggccgcgcgc agccagaacc gtttcttcgg
     2041 cggcgtgctg atggcggtag gggcgacgct gttcttcgtc aagcgcaacc cggctgccta
     2101 aaactgccgt cccgttttcc cacctaagcg atattgcgat taaggaggaa acatgtccgc
     2161 tgaggcattc acgttctggc agggactggg cgtcctgttc ttctggctgg tatttttgat
     2221 cccgctctgg cgcatcatct ccaaggccgg ctactccggc gcctgggccc tgctctcgct
     2281 gatccccctg gtcaacatcc tggcgctctg gatctttgcc ttcgcacgct ggcccagcga
     2341 acgggagcgc cgggaagacc acggctaccc gcatcgctga ttgccgcccc tgcacggccc
     2401 cgcgatagct gagccgggtc gacacccatc ctgccgccgc cggcggcagg aaatgcccgc
     2461 attgccattt tcgccatgcg gccggcttgc cattacaatc gcaccctgtc gacgcagccg
     2521 ccacactcac agggatcatc catgttcatg accaatatcg aaaccgttcc gggcaagacc
     2581 atcaccgcgg tgcatggcgt ggtcaccggc agcacggtgc gcgccaagca tgtcggacgc
     2641 gacatcatgg ccagcctgaa gaacatcgtc gggggggagc tgaagggcta taccgagctg
     2701 ctcgaagacg cgcgccgcga tgcgatcgag cgaatgcaat cccaggcgcg ccagatcggc
     2761 gcgaacgcca tcgtcaacgt gcgtttctcc acgtcggccg ttgccgccgg cgccgccgaa
     2821 atctacgtct acggcaccgc agtgacggtg gagtaggccg ccatggaagc cattgttctc
     2881 tggctgctgc tgctggtcgc tggctatgtg ttcggccagc gcgcagaacg caggcattac
     2941 cggtcgatcg aagcgcgcga gcgcgcgctc ctggcgctgc cggcaaccaa tgcgcgcacc
     3001 ccggtcgatc cgccgggacc ggtcgcgcgc gccgaactgg tccacggcag cgtcgtgatc
     3061 tcggtcgact tcttcaagcg gatcgccgcc ggcctgcgcg gcatcgtcgg cggccgcgtg
     3121 caatcctatg agaccctggt cgaccgggcg cgccgcgagg cggtgctgcg cctgaaggaa
     3181 agctgccccg gtgcgggcca ggtgattaac ctgcgcctgg agaccgcgac gatctccggc
     3241 ggcggaggcc gcgccctcgg ctcggtggaa gtgctggcct acggcacggc gctctacttc
     3301 gatacgcgcg aatgaaatac aagccggccc tcccggaaca gaacgacaac gtctcgcacc
     3361 gcaagccggt gcgcgagttt ttcctgatcc tcgcgggcct ctcgattgcc gcgctggccc
     3421 tgttctgggt gctcggtctg gcggtcgatc tcgcggtcga ccacatgagc gcgcaggcgg
     3481 aggcgcgcat ccatcgcgca ctgcgcgtct cctacccgca acacaagccg catgcgccgc
     3541 agatgcaggc gagactggaa cggatggtcg acgaactgcg cgcctgcgcg gacctgccgt
     3601 atccggtcac gctgcaactg gtgaaggcgg aacagccgaa tgcgggcgta ttacccggtg
     3661 cgcacatcct ggtgttttcc gggctgctgg acaagatcga gaccgagaac gcactagcct
     3721 tcgttctcgc gcatgagctt gcccacctga aaaaccgcga tcatctgcgc ggactcggac
     3781 gcacgctgct gctgcttgcg atctcgatca cgctgaccgg cacccactcg gacctcaccc
     3841 agatcctcgt gccggccaac cagctcggca tggcacgcca ctcgcaggcg cgggaaacgg
     3901 ccgccgacaa caccgccctc gacatcctgc attgccacta cgggcacgtc ggtggcgcga
     3961 ccgagttctt cgaggcgctg caccgacgcg ccggcgaggc gccggtaggt ctcggccatt
     4021 actttgcatc ccatccggaa ttgcgggcac gcatcgacaa cctgaaccgg cgtgccgagc
     4081 agcacgctta cccgatcggt ccgacgcgtg cgctcgcgcg ccagtagcgg tcccgaagag
     4141 gtgtgcgcgg cagcacctgg ggggagcagc gactcaagcg tcgagggcca ggcgggcaaa
     4201 gcgctgcagg atgcttgcgg cctgcgcggt catcccgacc tgcgcgcgca ggtcttccgg
     4261 ggatcgcccg cgcgcggcga gatcggtggc gaacttgtct atgtagccgt gcatcgcctc
     4321 ggcgtcgaac tccggatgga actgcacacc ccaggcgcac gcgccgaagc ggaatgcgtg
     4381 atgcgcgtcg tgctcgtttt ccgcaagtcg cacggcgcct gccggcaagc cgagcacggt
     4441 ctggctgtgc accgcctggg ccgggaagat ccggggaagc gatgcgaaca gaggatcatc
     4501 gacggcggca gcggtcttgc ggatctgcac ggtaccgatc tccatgcccc gcgggtgata
     4561 ggcaacttct ccgccgagcg cgtgggccag cagctggtgg ccatagcaaa tgcccaggat
     4621 tggcagcctt gcctcgaccg cgtgccgcag ccatggcacg agcgactcgc tccacggttc
     4681 gcggtccgtc accatcgccg cagacccagt cacggccgcg cccgcgagca ggcccgggtc
     4741 cggcaggtcg cacgcgcgcc gcgcatccac gatccgcacc ttcgaagccg caatcctgaa
     4801 accggccgca atccagtgtt cgaaatcgtg gccgcgctcg cgcgcgactg gcagcgcatc
     4861 gccaaccttg atgatgagca gaggtttcac ttttgtcagg gtcccatgtc ggctgtgccg
     4921 aagcacgatg atagcgggac gcggccgcgc atgcaggtga cgcctgttgc gcgatcgagt
     4981 tggcactcta ataaaattcc gcggtccgac gagttgcaca ggtaaagtac gcgatggcgc
     5041 aaaacctggt acaaggtgcg ccgtcgaaca tcaccagtcg gctcgccgcc ctgctgcccg
     5101 gggtggcgca aaagacaaca tacggaagga ggagggcttt cccatgaccg cttcaacggt
     5161 tttcgatcac tcgccctggg tggggcgcta caaaaacatc gactggcaac tgaaaacgcc
     5221 gcccgcagcc agcctgccgg cgatggtgag cgcggcgtcg cgcgcctacg cgaagcagaa
     5281 ggccttcacc tgcgtgatgc cgaacgggat gtacggcacg ctcacctacg aacagaccga
     5341 ccgcatgagc gacgacttcg ccgtctacct gcgcgaagtg ctgaagctgg ccc
//
LOCUS       DATLGF010000365         6957 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_74034,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000365 DATLGF010000000
VERSION     DATLGF010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6957
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74034"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="VM406_11225"
     CDS             <1..1075
                     /locus_tag="VM406_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVL76577.1"
                     /translation="GGREHQILAGLDVYQEDARRNNNFAGPPSGLTTTVGTPNDGAWR
                     ADTRGEPPFNTFDARNIGLYVQDMVSITPTVKLLGGLRFDRFTASYQTVAGGSNERSE
                     NLWSPRVGALYQPNPLSSYYASYGTSYNTAADTYQFTPGAPAPGSMAELEAQAPAEKS
                     RNIEIGGKWELFDNRASLGVAAFYSEKYNERNTDPDVQVVLLSGKRHAAGIELNLAGR
                     ITPQWEVFFNHTWIPDASIDRSNRTLNAAGTGFQVAGDRPALTPRYSGSLWTTYRVLP
                     QLRVGAGLTYRGSQNPEGARHMTADGFVVADAMAEYTFDQTWSARLNVSNLTDKLYAD
                     TLYRGFYGPGAPRRVQLTLKANF"
     gene            1086..1775
                     /locus_tag="VM406_11230"
     CDS             1086..1775
                     /locus_tag="VM406_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HVL76578.1"
                     /translation="MLLRLKNILTREELATARSLLEDSTAWIDGRSSAGGQAVARKNN
                     EQLAQDSELSRQLQAMILAAVQRDPLFFSAALPRKIFNPLFNRYSGDSNHYGAHIDGA
                     VLHSRNSGQWVRTDLSCTLFLAEPDEYDGGELVIQDALGEKRVKLPAGDMVLYPGTTL
                     HQVTPVTRGARLASFFWIESMVRSGEQRSLLFEMDMALLKLRTALGEQDPSVVQLTGT
                     YHNLLRMWADT"
     gene            1772..2884
                     /locus_tag="VM406_11235"
     CDS             1772..2884
                     /locus_tag="VM406_11235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HVL76579.1"
                     /translation="MKRLPAVETLPPGLVTLADHEQQAREHLDANAWAYFSGGAGDEI
                     TLRANRSGWDGMQLRTRVLRNLVGGHTRTTLLGRTLAHPILLAPVAYQRIAHPDGELA
                     AAYAAASHGAGMVLSSQASMPLEQIAAACLGEASRGPLWFQLYIQHDRGFTRELVKRV
                     EQAGYEALVVTVDAPSSGVRDRELRAGFRLPVGISAVNLAGLTAPPALALRPEQSRMF
                     DGLLTSAPTWADIEWLKSCTRLPILLKGIMHEEDAREALRLGAAGVIVSNHGGRTLDT
                     MPPTCAVLPRVREAIGADAAVLVDGGIRRGTDVIKAIALGADAVMIGRPYVFGLANAG
                     AIGVSHVLRLLRDELEIAMALCGCKTLAEADARILF"
     gene            3067..3981
                     /locus_tag="VM406_11240"
     CDS             3067..3981
                     /locus_tag="VM406_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HVL76580.1"
                     /translation="MIRRTLTALLLCLSASAFALPFSSQHVLVYEEETGKILLEKNAS
                     TVVPIASLTKVMTAMVVLDAGLDMDEPIRITETDVDTLKFSSSRVPVGVVLTRKSLLE
                     LSLMSSDNRAAAALGRTFPGGHAGFREAVRRKAGELGMRDTYIEEPTGLSPNNRSSAL
                     DLLRMVAAASAYPEISRMSTGLGEMMDMNGRTVEYRNTNRLIGRDGWDILLSKTGFTR
                     EAGRCLVMRFQSAGRHVIVVLLNASESAARMLDAENVQRYLNGESPVAAAPRAAPQAR
                     AAKPAVVRVSNGKKPNKPQQQTKRRTST"
     gene            complement(4036..4473)
                     /locus_tag="VM406_11245"
     CDS             complement(4036..4473)
                     /locus_tag="VM406_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVL76581.1"
                     /translation="MFKKFLVPTDGSTQSAKAVQGAIQLAGQVGGSLVALAVVEPYPF
                     PAISESPYAGGSAAYEERALDLAKDHVEQVAAAAAKAGIPCETVVLHGLYPHEEIVAA
                     ALGRSCDAILMGTHGRRGISRLFAGSETQSVIGQSPIPVMVFR"
     gene            4663..5100
                     /locus_tag="VM406_11250"
     CDS             4663..5100
                     /locus_tag="VM406_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVL76582.1"
                     /translation="MFSKILVPTDGSELSERAGRAAVAFAAQQRAAIVALCVAEPYPF
                     APLAETAFVDEREDFEKRARALAQEHVNRIASAAADAGVSCSTVVAQSHAPYDEIVKT
                     AAEQGCDLIFMASHGRKGLSRLFIGSETQKVLANSSIPVMVYR"
     gene            5188..5613
                     /locus_tag="VM406_11255"
     CDS             5188..5613
                     /locus_tag="VM406_11255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HVL76583.1"
                     /translation="MSTVNWSPDMRLGIPRLDRAHEAFMRELERLREIPDAHFGPAFH
                     SMVVRMEGDFQEEEALMEDIDFPGLHVHREQHARVLGALHHVAARVMGGDYASGREAT
                     DLLEQWFLMHLSTMDTVLALTLEVIEAERAESLSADRSL"
     gene            5625..6101
                     /locus_tag="VM406_11260"
     CDS             5625..6101
                     /locus_tag="VM406_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76584.1"
                     /translation="MLNLRLAPIWHGFLGALLLAASGLVAADRTAVGGVYVAGQGFTI
                     EQALAQALNEQQQGAPFWILAAGDEARRIVDAGRADTRMLLAEIRDRGGLVYACERDL
                     PPGKEIALPAGVGVVAPPANGAPRLFAPAEIEDDEPLLPESVRQNRLMLRTCAAFH"
     gene            6438..>6957
                     /locus_tag="VM406_11265"
     CDS             6438..>6957
                     /locus_tag="VM406_11265"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HVL76585.1"
                     /translation="MNLRSVRIGTRLGIGFGIILVLLLAVVGIDIVASTASRHAAAEG
                     MRHANAKAVVAGTMRSAILEGGIAMRNVGLQYSTPEMEREEAKLLVQRKRFKEASEKL
                     TALGLAPEEKALLQGIEALDAQTEAPFRQAIEKVKEYANEAAGKLISSEVDPLNQKAI
                     LEIDKLVDMQQAE"
BASE COUNT         1247 a         2219 c         2270 g         1221 t
ORIGIN      
        1 gggcggccgc gagcaccaga ttctcgccgg cctcgacgtg taccaggagg atgccaggcg
       61 caacaacaac ttcgcaggcc ctcccagcgg gttgacgacc acggtcggaa cgccgaatga
      121 tggcgcctgg cgcgcagaca cccgcggcga gccgccgttc aataccttcg atgcccgcaa
      181 catcggcttg tacgtgcagg acatggtaag cattacacct accgtgaagc tgctcggcgg
      241 cctgcgcttc gaccgcttca ccgcctccta ccagacggtt gccgggggga gtaacgaacg
      301 cagcgagaac ctctggtcgc cacgcgtcgg cgcactgtac cagccgaatc ccttgtcttc
      361 gtattacgcg tcctacggca cctcgtataa cacggccgcc gacacctatc agttcacgcc
      421 gggtgctccg gcgccaggat cgatggcaga actggaggcg caggcgcccg ctgaaaagag
      481 ccggaatatc gaaattggcg gcaagtggga gctgttcgac aatcgtgcgt cactcggcgt
      541 tgcggcgttc tatagcgaga agtacaacga acgcaatacc gacccggatg tgcaagtggt
      601 tctcctgtca ggcaagcgac atgccgctgg catcgagctg aatctcgccg gccgcatcac
      661 gccgcaatgg gaagtcttct tcaatcacac gtggattccc gacgcaagta tcgaccgaag
      721 caacaggacc ctcaatgcag ccggcacggg cttccaggtc gccggggatc gtccggcgct
      781 gacgccacgc tacagtggca gcctgtggac cacctaccgc gtgctgccgc agctgcgcgt
      841 cggagccgga ctgacctatc gcggatcgca gaatcccgag ggtgcccgcc acatgacggc
      901 cgatggtttc gttgttgccg atgccatggc cgagtacacc ttcgatcaaa cctggtccgc
      961 gcgtctgaac gtcagtaacc tgacggacaa gctgtatgcc gacacgctct accgtggctt
     1021 ttacggcccg ggggcgccgc ggcgtgtaca gttgacgctc aaggcaaact tctaagaggc
     1081 ccggcatgct gctgcgcttg aaaaacatcc tgacccggga ggaactcgcc acggcgcgtt
     1141 cgttactcga ggacagtacg gcctggatcg acgggcgcag cagcgcgggc ggccaggcgg
     1201 tcgcccgcaa gaacaacgag caactggcac aggacagcga gctctcgcgg cagttgcagg
     1261 ccatgatcct ggcggcggta cagcgcgacc cgctgttctt ctcggcggcg ctgccgcgca
     1321 agatattcaa cccgctgttc aaccgctaca gcggcgacag caaccactac ggcgctcaca
     1381 tcgacggcgc cgttcttcat tcgcgcaatt ccgggcaatg ggtgcgcaca gacctgtctt
     1441 gcacactgtt tctcgccgaa cccgacgagt acgatggcgg ggaactggtg atccaggatg
     1501 cactgggcga aaagcgcgtg aagctgcctg ccggcgacat ggtgctgtac ccgggcacca
     1561 cgctgcacca ggtcacgccg gtcacgcgtg gtgcgcgcct ggccagtttc ttctggatag
     1621 aaagcatggt gcgatcgggt gagcagcgca gcctgctgtt cgagatggac atggcattgc
     1681 tcaagctgcg caccgcgctc ggcgagcagg atccctcggt ggtgcagctc accggcacct
     1741 atcacaacct cctgcgcatg tgggctgaca catgaagcgc ttgcccgccg tggaaacgct
     1801 tccgcccggc ctggtcacgc tggccgatca cgaacaacag gcgcgcgagc acttggacgc
     1861 caatgcctgg gcctacttca gcgggggtgc aggggacgag atcacattac gcgcgaaccg
     1921 cagcggctgg gacggcatgc agctgcgcac gcgtgtgttg cgcaatctgg tcggcggtca
     1981 tacgcgcacc acgctgctcg gtcgcacatt ggcgcatccg atactgctgg cgccggtcgc
     2041 ttaccagcgc atcgcgcatc cggatggcga attggccgcc gcctatgccg ccgcctcgca
     2101 cggcgccggc atggtgctca gttcccaggc cagcatgccg ctcgagcaga ttgctgccgc
     2161 ctgtctcggt gaagcctcgc gcggtccgct ctggttccag ctctacatcc agcacgaccg
     2221 cggcttcacg cgcgaactgg tgaaacgagt cgaacaggcg ggatacgaag cgctggtcgt
     2281 gacggtcgat gcacccagca gcggcgtgcg tgaccgcgaa ctgcgcgccg gcttccgcct
     2341 tcccgtgggc atttcggctg tcaatctcgc aggtctcacg gcccctccag cgcttgcgct
     2401 acgaccggaa caaagccgga tgttcgatgg cttgctgacg tcggcgccga cctgggccga
     2461 catcgagtgg ctgaaatcat gcacgcggct gccgattctt ctgaagggaa tcatgcacga
     2521 ggaggacgcg cgcgaggcgc tgcggctcgg cgcggccggc gtgatcgtct ccaaccacgg
     2581 cgggcgcacg ctcgacacga tgccgccgac ctgcgccgta ctcccgcgcg tacgggaagc
     2641 aatcggcgcc gatgcggcag ttctggtgga tggcggcatc cggcgcggca ccgatgtcat
     2701 caaggccatc gcgctggggg cggacgcggt catgatcggg cggccttacg tgttcgggct
     2761 ggccaatgcc ggcgccatcg gtgtttcgca tgtgctgcgg ctgctgcgcg acgagctgga
     2821 gatcgcgatg gcgctgtgcg ggtgcaagac cctcgccgag gcggacgccc gcattctttt
     2881 ctgaccgccg aagcgttgtt tcatttcggc acggcaatgt cccctcgtca agccttgaaa
     2941 aggcttcaag gtttctccgc aacattcaga aatatttccc tgatctagta ttttcctcaa
     3001 tgcattgcgc tgcattaacg cgctatgatt ggtgccacgt aacaatcttt tccgtgtgca
     3061 ccaggaatga tcagaagaac cctgacggcg ctgctcctct gcctctccgc gtccgcattc
     3121 gcgttgccat tcagttccca gcacgtcctc gtttatgaag aggaaactgg gaagatactg
     3181 cttgagaaaa atgccagcac ggtggtgccg atcgcgtcgc tgacgaaggt gatgacggcg
     3241 atggtggtgc tcgatgcggg cctggacatg gacgagccga tccggatcac cgaaaccgac
     3301 gtcgatacgc tcaagttcag ttcctcccgc gtgccggtcg gcgtggtcct gacgcgcaag
     3361 agcctgctcg agctttcatt gatgtcgtcg gacaaccgcg ccgccgccgc gctcggccgc
     3421 accttccctg gcggacatgc gggcttccgt gaagcggtgc gccgcaaggc cggcgaactc
     3481 ggcatgcgcg acacctacat cgaagagccg accggcctgt cgccgaacaa ccgttcctcc
     3541 gcgctcgatc tgttgcgcat ggtggcggcg gcgtccgcct atccggagat cagtcgcatg
     3601 tcgaccggcc tcggcgagat gatggacatg aacggacgca cggtcgagta tcgcaatacc
     3661 aatcgcctga tcggccgcga cgggtgggac atcctgcttt cgaagaccgg cttcacccgg
     3721 gaagcagggc gttgcctcgt gatgcgcttc cagtccgcgg gccgccacgt gatcgtggtg
     3781 ctgctcaacg cgagcgagag cgctgcccgc atgctggatg ccgagaacgt gcagcgatac
     3841 ctgaacgggg agtcgccggt cgccgcggcg ccgcgcgccg cgccgcaggc gcgtgccgcg
     3901 aagccggctg tcgtgcgcgt gagcaacggc aagaagccga acaagccgca gcagcagacg
     3961 aagcggcgca cgtcgacctg agcgcgaccc atcacatatg cgaagagccg gaccatggtc
     4021 cggctttttt cacgcctacc gaaacaccat gaccggtatc ggggactgcc cgatcacgct
     4081 ctgcgtctcg ctgccggcga acaggcggct gattccgcgc cgtccgtgcg tgcccatcag
     4141 aatcgcgtcg cagctgcgcc cgagggccgc tgcgacgatt tcttcatgcg gatacaggcc
     4201 atgcaagacc acggtttcac atggaatacc ggccttcgcc gcggcggccg ccacctgctc
     4261 cacatgatcc ttcgccaggt ccagcgcgcg ctcctcatac gcggctgatc cgccggcata
     4321 cgggctctcc gagatagccg gaaatggata cggttcgacc acggcgagcg cgacgaggct
     4381 gccgccgacc tggccggcca gctgtatcgc gccctggacg gctttcgcgc tttgggtgga
     4441 gccatcggtg gggacaagga atttcttgaa catgtgcgtg tcctgaagca aacggatgga
     4501 tggagagtgc gccggttgcc gccgcatttc gttgatccag ctcaagtccg ggcgcgggaa
     4561 cccgggcagg tttgccgcgt cggattgcgt gccggttcgc cttcaagaga cggaagtgcg
     4621 ggccccgtat tgtcatggcg tcgtcttctg ccgggaggaa tggtgttcag caagattctg
     4681 gtcccgacgg acggctccga actgtcggag agggcggggc gggcagccgt cgcgttcgcg
     4741 gcccagcagc gtgccgcgat agtcgccttg tgcgtcgccg aaccgtatcc gttcgcgcca
     4801 ctggcagaga cggctttcgt cgacgaacgc gaggatttcg agaagcgcgc gcgtgcgctt
     4861 gcacaggagc atgtcaaccg tatcgcgtcc gccgcggccg atgccggcgt ctcttgctcc
     4921 acggtggttg cgcagtcgca tgcgccgtac gatgaaatcg tgaagacagc ggccgagcag
     4981 ggatgcgacc tgatcttcat ggcgtcccac gggcgcaaag gactgagccg gctgttcatt
     5041 ggcagcgaaa cgcaaaaagt actggcaaat tcgtcaatac ctgtcatggt atatcgatag
     5101 gcacagggat tctgccttca attcggaagt gagcctgcca tgcgcagggc ttccgcattg
     5161 aagtgccgtt acccgaggag agatccaatg agtaccgtca actggtcgcc ggacatgcgg
     5221 ctgggcattc cccgtctcga tcgagcccac gaagccttca tgcgcgagct ggagaggctg
     5281 cgcgagattc cggatgcgca cttcggcccg gccttccact ccatggtcgt ccgtatggag
     5341 ggcgacttcc aggaggagga agcgttgatg gaagacatcg atttccccgg cctgcatgtg
     5401 caccgcgaac agcacgcgcg cgtacttggg gccctgcatc atgtcgctgc acgcgtgatg
     5461 ggtggcgact acgcatccgg ccgcgaagcg accgacctgc tggaacaatg gttcctgatg
     5521 cacctgtcga cgatggatac ggtgcttgcc ctcacgctgg aagtgatcga ggctgaacgc
     5581 gcggaaagcc tcagcgccga ccgctccctt taaggatcgg ggcgatgctg aacctgcggc
     5641 tggctccgat ctggcatggc ttcctggggg cgctgctgct ggccgcgtcc gggctcgtgg
     5701 ccgcagaccg tactgctgtt ggcggcgtct atgtcgcggg gcagggattc accatcgagc
     5761 aggcgctggc gcaggcgctc aacgagcagc agcagggcgc gccattctgg atcctcgccg
     5821 cgggcgacga agcgcgccgc attgtcgatg ccgggcgcgc cgatacgcgc atgctgctcg
     5881 ccgagatccg cgaccgcggc ggcctcgtgt acgcgtgcga gcgcgacctt ccccccggca
     5941 aggagatcgc gttgcccgcc ggcgtcggcg tggtcgcgcc gccggctaac ggggcgccgc
     6001 gtttattcgc gccggccgag atcgaagatg acgagccgct gctgccggag agcgtgcggc
     6061 agaacaggct gatgctgcgc acctgcgcgg ccttccattg atgtgcaatt gcaacgcgtc
     6121 gtgcctgtcc tgaaatcagg cagggtgacc ggtgtccggg tttcatgcac ccgcggtgac
     6181 ccgcttcgtc attgacctca gccgggcccc ttccatggcc tgagcgacgt aacttgccgg
     6241 ctcccgtggt cttggggcgg cttccggttc ccgctgcgag tctgccccac gccgcgttgc
     6301 ggggtgatgt gtacacgcgc cggacagttc tgcttgtgcg tgttttccag acggcaataa
     6361 aatctgccca ccccggagtg actcccgtac actgccagta acaaaaacac gcgcgcgacg
     6421 cgcgagggag gctccgcatg aatctacgca gcgttcgaat cggcacccgg cttggcatcg
     6481 ggttcggcat catcctggtg ctgctgctgg cagttgtcgg catcgatatc gtggccagta
     6541 ccgccagccg ccacgcggcc gccgaaggga tgcgccacgc gaacgcgaag gcggtggttg
     6601 ccggcacgat gcgcagcgcc atcctcgagg gcgggatcgc gatgcgcaat gtcgggctgc
     6661 agtacagcac gccggagatg gagcgcgagg aggccaagct gctggtgcag cgcaagcgct
     6721 tcaaggaggc gagcgagaag ctgaccgcgt tgggtctcgc gccggaggag aaggcgctgc
     6781 tccagggcat cgaggcgctg gacgcgcaga ccgaggcgcc gttccggcag gccatcgaga
     6841 aggtcaagga gtatgcgaac gaggcggccg gcaagctgat ttcctcagag gtcgatccac
     6901 tgaaccagaa ggccatcctg gagatcgaca agctggtcga catgcagcag gcggagg
//
LOCUS       DATLGF010000366         5843 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_197013, whole genome shotgun sequence.
ACCESSION   DATLGF010000366 DATLGF010000000
VERSION     DATLGF010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197013"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VM406_11270"
     CDS             complement(<1..435)
                     /locus_tag="VM406_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HVL76586.1"
                     /translation="MNRYPLWKYAIIVIALVIGALYTVPNFFGESPAVQVSSAKATIN
                     VEPALVQRVEQVLIQGGAPPQRVYYETIGTQGTIRALFADTDTQFRARALLERELNAN
                     PADPTYLVAFNLLPNTPQWLQSLGAAPMYLGLDLRGGVHFLMQ"
     gene            complement(516..785)
                     /gene="yajC"
                     /locus_tag="VM406_11275"
     CDS             complement(516..785)
                     /gene="yajC"
                     /locus_tag="VM406_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HVL76587.1"
                     /translation="MLSFLPIILMFVVLYFLMIRPQMKRQKEHRAMMDALTKGDEVVT
                     AGGIVGRVTKVTDAYVTVEINEGNEVLVQKNAVATLLPKGTIKTL"
     gene            complement(937..2064)
                     /gene="tgt"
                     /locus_tag="VM406_11280"
     CDS             complement(937..2064)
                     /gene="tgt"
                     /locus_tag="VM406_11280"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HVL76588.1"
                     /translation="MLKFELLKTDGRARRGRLTLNHGVVETPIFMPVGTYGSVKAMSP
                     LELKEIDAQIILGNTFHLWLRPGLDVVGKFGGLHRFIGWDKPILTDSGGFQVFSLGAM
                     RKITEEGVKFASPINGDKLFLSPEISMQIQKVLNSDIVMQFDECTPYAIDGRPATEQE
                     AGKSMRMSLRWARRSRDEFDKGENPNALFGIVQGGMYEHLRDESLEGLKEIGFDGYAI
                     GGLSVGEPKQDMMRVLEHVGPRLPADKPHYLMGVGTPEDLVAAVANGVDMLDCVMPTR
                     NARNGWLFTRFGDIKIKNSRYKDDDRPLDESCDCYTCRNFSRAYLYHLHRAGEILGAR
                     LNTIHNLHYYLQLMREMRAAIDADRFADFATRFAADRARGA"
     gene            complement(2058..3083)
                     /gene="queA"
                     /locus_tag="VM406_11285"
     CDS             complement(2058..3083)
                     /gene="queA"
                     /locus_tag="VM406_11285"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HVL76589.1"
                     /translation="MYSLSDFDFHLPEELIAQFPLPERSASRLLQVEADRLLDRRFTD
                     IADLLAPGDVLVFNDTRVLKARFFGVKGTGGRIEALVERVLDDRTVLAQVRASKTPQP
                     GSRIRLADAFDVTVGERVGEFFTLAFPSDVFALIDAHGSLPLPPYIEHAADAVDEQRY
                     QTVYAKEPGAVAAPTAGLHFDEALLETLRAKGVGQAFITLHVGAGTFQPVRSENLSEH
                     KMHSEWYTIPQATVDAVRTAQAAGRNVVAVGTTSMRALESASRSGALRAGSDDTNLFI
                     TPGYRFNTVTQLITNFHLPKSTLLMLVSAFAGYDRIKAAYAHAIEQRYRFFSYGDAML
                     LTRNTEC"
     gene            3214..5292
                     /gene="recG"
                     /locus_tag="VM406_11290"
     CDS             3214..5292
                     /gene="recG"
                     /locus_tag="VM406_11290"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HVL76590.1"
                     /translation="MPAASRKTAAPAPAQTAKTAENKLARLGLRTDMDLVLHLPMRYE
                     DETRLLPIREAGFMSGGTAQVEGTVQSCEVQYRPRRQLVVTIADESGQLVLRFLNFYG
                     SQVKQLAPGTRVRVRGEVRSGFFGAEMVHPSYRVVQEGDPLPQSLTPVYPAGEGLSQA
                     YLRKAIANAMTRVDWRDTLGDELRQALQLAPFEASVRLLHHPPPDVSEHALAERSHPA
                     WVRMKFDELLAQQLSLKRAQAARRTKGAPPLTVEGELAHGLLARLPFRLTAAQQRVLD
                     EIRADLAAPYPMQRLLQGDVGSGKTVVAALAAAQAIDSGYQAALMAPTVILAEQHFRK
                     VAQWLEPLGVPIAWLTGSLKKKEKEAARDAVESGVARLVIGTHALIQEDVVFEQLGLV
                     IVDEQHRFGVSQRLALRNKGFEAGHALVPHQLMMSATPIPRTLAMTYYADLEISVIDE
                     LPPGRSPVITRLVDQNRREEVIARVHAAAREGRQVYWVCPLIEESEALQLQTATDTWT
                     TLCAALPDLQVGLVHGRLKPAEKQEVMEAFSAGRIHLLVATTVIEVGVDVPNASLMVI
                     EHAERFGLSQLHQLRGRVGRGAAQSVCVLLYQSPLGPIAKERLKIMRETTDGFEIARR
                     DLEIRGPGEFLGARQSGQAMLRFADLQTDQWLVEEARNVAERLLREDPATVDAHLKRW
                     LGSREEYLKA"
     gene            5324..>5843
                     /locus_tag="VM406_11295"
     CDS             5324..>5843
                     /locus_tag="VM406_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVL76591.1"
                     /translation="MTLTELKYIVAVAREKHFGHAAEACFVAQPTLSVAIKKLEEELG
                     VLIFERGGGEVSLTPIGAQIVAQAERVLEQTAAIKEIAKQNKDPLAGPLRLGVIYTIA
                     PYLLPPLVKTLIESVPQMPLVLQENFTVRLIESLRQGELDAAIMALPFNEQGLNVVPL
                     YDEPFVVAVPSQH"
BASE COUNT         1058 a         1858 c         1947 g          980 t
ORIGIN      
        1 ctgcatgagg aagtgcacgc cgccgcgcaa atcaaggccg agatacatcg gcgccgcgcc
       61 gaggctttgc agccattgcg gagtattggg cagcaggttg aacgcgacca ggtaggtggg
      121 gtcggccgga ttcgcattga gctcgcgctc gagcagcgcg cgtgcacgga actgggtatc
      181 ggtatcggca aacagcgcac ggatcgtgcc ctgggtgccg atcgtctcgt agtagacgcg
      241 ctgcggcggc gcccctccct ggatcagcac ctgttccacc cgctgcacga gggcgggctc
      301 gacgttgatg gtcgccttgg cgctgctgac ctgcaccgcc ggagactcgc cgaagaagtt
      361 gggcacggtg tacagcgcgc cgatcaccag tgcgatgacg atgatggcgt atttccagag
      421 aggatagcga ttcataaggc tcgactgaca aagaaagcgg ggcgcaggat ggagccccgc
      481 ccgggcacgc gacgcgcccg gtgagcgctt cgaccttaca gggtcttgat ggtgcccttg
      541 ggaagcaggg tggcgacggc gttcttctga accaagactt cgttgccttc gttgatctcg
      601 acggtgacat aggcgtcggt gaccttggtg acgcggccga cgatgccgcc ggcagtgacg
      661 acttcatcgc ccttggtcag cgcatccatc attgcccggt gttccttctg gcgcttcatc
      721 tgcggacgga tcatcaggaa gtacagcacg acgaacatca ggatgatcgg caggaagctc
      781 agcatgccgg cttctgctcc gcctgcggct tgggcgtatg cgttggaaat gaacacagtg
      841 tcctcttatt gaagtggttg gggaaaatca ggccggcatt ttagcacccg caagcacggg
      901 caccccgccg gcgcagcctg ccggaaactg cggttttcat gcgccgcgcg cccggtctgc
      961 ggcaaagcgc gttgcgaagt cggcaaagcg gtccgcgtct atggccgcgc gcatctcgcg
     1021 catcagctgc aggtagtaat gcaggttatg aatcgtgttc aggcgcgcac cgaggatttc
     1081 gccagcgcgg tgcaggtgat acaggtaggc gcgcgagaag ttgcggcagg tgtaacaatc
     1141 gcagctctcg tcgagcggcc ggtcgtcgtc cttgtagcgt gaattcttga tcttgatgtc
     1201 accgaagcgg gtaaacagcc agccgttgcg ggcgttgcgg gtaggcatca cgcagtcgag
     1261 catgtcgacg ccgttggcca ctgccgccac cagatcttcc ggcgtaccca cgcccatcag
     1321 gtagtgcggc ttgtcggccg gcagacgcgg gccgacatgc tcgagcacgc gcatcatgtc
     1381 ttgcttcggc tcgccgaccg aaaggccacc gatcgcgtag ccgtcgaaac cgatttcctt
     1441 aagcccctcc agcgactcgt cgcgcaggtg ctcgtacatc ccgccctgga cgatgccgaa
     1501 cagcgcgttc gggttttcgc ccttgtcgaa ttcgtcacgg gagcgccgcg cccagcgcag
     1561 cgacatccgc atcgacttgc cggcttcctg ctcggttgcc ggacgcccgt cgatcgcata
     1621 gggtgtgcac tcatcgaact gcatgacgat gtcggaattg agcaccttct ggatctgcat
     1681 cgagatttcc ggcgaaagga acagcttgtc gccattgatc ggtgatgcga atttcacacc
     1741 ttcttcagtg atcttgcgca tcgcgcccag cgaaaacacc tggaagccgc cggagtcggt
     1801 caggatgggc ttgtcccagc cgatgaagcg atgcaggccg ccgaacttgc cgaccacatc
     1861 gagccccggg cgcagccaca ggtggaaggt gttgccgaga atgatctgcg catcgatctc
     1921 cttcaactcc agcggcgaca tcgccttgac agagccgtag gttccgaccg gcatgaagat
     1981 cggcgtttcg acaacgccgt ggtttagcgt gagacggccg cggcgcgcgc ggccatcggt
     2041 cttgagcagt tcgaatttca gcattcggtg ttcctggtca gaagcattgc atcgccatag
     2101 ctgaagaagc gataacgctg ttcgatcgca tgagcgtagg cggccttgat gcggtcatag
     2161 ccggcgaagg ccgacaccag catcaggagc gtagacttgg gcaggtggaa attggtgatc
     2221 aactgcgtga cggtgttgaa tcggtagccc ggcgtgatga acagattggt atcgtcactg
     2281 ccggcgcgca gcgcgccgga gcgcgaagcg gattcgagcg cgcgcatgct ggtggtgccg
     2341 accgcgacca cattgcgccc ggccgcctgc gcggtccgca ccgcgtcgac ggtcgcctgc
     2401 ggaatcgtgt accactcgct gtgcatcttg tgctcggaga ggttttcgct gcgcaccggc
     2461 tggaacgtgc cggcgccgac gtgcagcgtg atgaacgcct gccccacgcc tttcgcgcgc
     2521 agcgtttcca gcaacgcctc gtcgaaatgc aggccggcgg tgggcgccgc caccgccccc
     2581 ggctccttcg catagacggt ctggtaacgc tgctcgtcga ccgcgtcggc ggcatgctcg
     2641 atgtagggcg gcagcggcag actgccatgc gcatcgatca gcgcgaacac atcggacggg
     2701 aaggcaagcg tgaagaattc gccaacgcgc tcgcccaccg tcacgtcaaa cgcatcggcc
     2761 aggcggatgc gcgagccggg ctgcggcgtc ttggacgcgc gcacctgggc cagcaccgta
     2821 cgatcgtcga gcacgcgctc aaccagcgcc tcgatccggc cgccggttcc cttcacaccg
     2881 aaaaagcgcg ccttcagcac gcgcgtgtcg ttaaacacga gcacgtcgcc cggcgcgagc
     2941 aggtcggcga tgtcggtgaa ccggcgatcg agcaggcggt cggcctccac ctgcaacagg
     3001 cgcgaggcgc tgcgctcggg gagcggaaac tgcgcgatca gttcctcggg caggtgaaag
     3061 tcgaaatcgg aaagcgaata catggaaaca agggattgcc agaattgcac agccgcgaac
     3121 gcattccgta caatgactgc ctacattgac tgtggcaccc atacgccggc tgcgaacaaa
     3181 cccgctattt tacgccggcc cgcccgtttc ccgatgcccg ctgcttcgcg caagaccgcc
     3241 gcgcccgctc ccgcccagac cgcgaagacc gccgagaaca agctcgcgcg cctcgggctg
     3301 cgcaccgaca tggatctcgt gctccacctg ccgatgcgct acgaggacga gacccggctg
     3361 ctgccgatac gcgaggccgg tttcatgagc ggcggcaccg cgcaggtcga gggcaccgtg
     3421 cagtcctgcg aagtgcagta tcgtccccgg cgccagctgg tcgtgacgat cgcagacgaa
     3481 tccgggcagc tggtgctgcg ctttctgaat ttctacggaa gccaggtcaa gcagctcgcc
     3541 cccggcaccc gtgttcgggt gcgcggcgag gtgcgcagcg gtttcttcgg tgcggagatg
     3601 gtgcatccga gctaccgggt ggtgcaggag ggcgatccgc tgccgcagtc gctgaccccg
     3661 gtctacccgg ccggcgaagg cctgtcgcag gcctacctgc gcaaggcgat cgccaatgcg
     3721 atgacacgcg tggactggcg cgacacgctc ggcgacgagc tgcgccaggc cctgcagctg
     3781 gcgcctttcg aagcttcggt gcggctgctg caccatccgc cgccggatgt gagcgaacat
     3841 gcgctggccg aacgcagcca tccggcctgg gtgcgcatga agttcgacga actgctggcc
     3901 cagcagctgt cgctcaagcg cgcgcaggcc gcgcgccgca ccaagggcgc gccgccgctg
     3961 acggtggaag gtgagctggc gcatggactg ctcgcccgcc tgccgttccg gctgaccgcc
     4021 gcgcagcagc gcgtgctcga cgaaatccgc gccgatctcg ccgcgcccta cccgatgcag
     4081 cggctgctgc agggcgacgt gggcagcggc aagaccgtgg tcgccgcgct ggccgccgcc
     4141 caggccatcg acagcggcta tcaggccgcg ctgatggcgc ccaccgtgat cctcgccgag
     4201 cagcatttcc gcaaggtcgc ccaatggctg gagccgctcg gcgtgccgat tgcctggctg
     4261 accggcagcc tgaagaaaaa ggagaaggag gcggcgcgcg acgcggtcga atcgggagtc
     4321 gcacgactgg tgatcggcac gcatgcgctg atccaggaag acgtggtgtt cgaacagctg
     4381 ggactggtga tcgtcgacga gcagcatcgc ttcggcgtaa gccagcggct ggccttgcgc
     4441 aacaagggct tcgaggcggg ccatgcgctg gtgccgcacc agctgatgat gtcggccacg
     4501 ccgattccgc gcacgctggc gatgacgtat tacgccgacc tcgaaatctc ggtgatcgac
     4561 gagctgccgc cgggccgctc gccggtgatc acgcgcctgg tcgaccagaa ccggcgcgag
     4621 gaagtgatcg cgcgcgtgca cgcggcggcg cgcgaggggc ggcaggtgta ctgggtgtgc
     4681 ccgctgatag aggagtccga ggcgctgcag ctgcagaccg cgaccgacac ctggacgacg
     4741 ctgtgcgcgg cgctgccgga cctgcaggtc ggcctcgtgc acggccgctt gaaaccggcc
     4801 gagaagcagg aagtgatgga ggcattcagc gccgggcgca tccacctgct ggtggcgacg
     4861 accgtcatcg aagtcggggt cgacgtgccg aatgcctcgc tgatggtgat cgagcatgcc
     4921 gagcgcttcg gcctgtcgca actgcaccag ctgcgcggac gcgtgggacg cggcgccgcc
     4981 cagagcgtat gcgtactgct ctaccaatca ccactcggac cgatcgcgaa ggagcggctg
     5041 aagatcatgc gcgaaaccac cgacggcttc gagatcgcgc ggcgcgacct ggaaatccgc
     5101 ggccccggcg agttcctcgg cgcgcgccag tcgggccagg cgatgctgcg ctttgccgat
     5161 ctgcaaaccg accagtggct ggtggaagag gcgcgcaatg tcgccgaacg gctgctgcgc
     5221 gaagatccgg cgaccgtcga tgcccatctg aaacgctggc tcgggagccg cgaggaatac
     5281 ctgaaggcat agccgacgga tagttggaga tacactagct gccatgacgc tcaccgaact
     5341 caaatacatc gtcgcggttg cccgcgaaaa gcatttcggc catgccgccg aagcctgttt
     5401 cgttgcccag ccgacgctct ccgtcgccat caagaagctg gaggaggagc tgggcgtgct
     5461 gatcttcgag cgcggcggcg gagaggtgtc gcttacgccg atcggcgcgc agatcgtcgc
     5521 ccaggccgag cgggtgctgg aacagaccgc ggcgatcaag gaaattgcca aacagaacaa
     5581 ggacccgctt gcaggcccct tgcgcctggg cgtgatctac acgatcgcgc cctatctgct
     5641 gccgccgctg gtcaagaccc tgatcgaaag cgtgccgcag atgccgctgg tgctgcagga
     5701 aaacttcacc gtgcgcctga tcgaatcgct gcgccagggc gaactggatg ccgcgatcat
     5761 ggccctgccg ttcaatgaac aagggctgaa cgtggtcccg ttgtacgacg agccgttcgt
     5821 ggtagcggtg ccgagccagc atc
//
LOCUS       DATLGF010000367        11042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_7842,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000367 DATLGF010000000
VERSION     DATLGF010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 11042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11042
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7842"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..529)
                     /locus_tag="VM406_11300"
     CDS             complement(176..529)
                     /locus_tag="VM406_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76592.1"
                     /translation="MELNRRNQAVLARSFASEAEHQEIERQLASKAHSRDIDARVARA
                     RAATSAPRDVGLQDITMLSVSPILRSLHNWWLKRAERHYLICADVELQRMREAQMNIA
                     YYQKRAAMARSARSV"
     gene            835..1905
                     /locus_tag="VM406_11305"
     CDS             835..1905
                     /locus_tag="VM406_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HVL76593.1"
                     /translation="MTDLQASLLIIGGTIVVGVISYNKWQEYKARKSVQKAFSSDHDD
                     VLMESDIAGAEAETRREPSLDIPAAHAGPEPIAEETNEAAASAKPAIQKELPIDELID
                     GAVELILEAPVRGEKLLPKLQTLRHVGSKPVHFVGQREDGVWEEIAVGGIYYGLYAGI
                     QLAGRNGPLNELEFSELITRLRQISDEIDAEPDVPDMSDVMARARALHQFVSEYDAQL
                     SISVRSKGAPWAINTLLAALERQGFDLRPDGRLVMPDGDGGVLFTLSTNVTLADETTP
                     RLTLLLDVPRVAPLRDGFGAMVACARMLATRLDGVVVDDSDQPLLEPALAEISEQVTA
                     FYTNMESAGVPAGSTRALRLFA"
     gene            1914..3983
                     /gene="ligA"
                     /locus_tag="VM406_11310"
     CDS             1914..3983
                     /gene="ligA"
                     /locus_tag="VM406_11310"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HVL76594.1"
                     /translation="MQPDFFGGPVPPPLRDPEADGWQERAAWLKDELNRHSHAYYVLD
                     NPTIPDAEYDRMFRELVELEQAHPDLVTSDSPTQRVGGKPLPQFEQVRHAVPMLSLNN
                     AFDDDEVRGFDRRVCEALQADNVAYATELKFDGLAVNLRYENGVLVQAATRGDGTTGE
                     NVTANIRTVRAIPLRLHGRDVPKVIDVRGEVLMFKADFARLNERQRAAGQKEFANPRN
                     AAAGSLRQLDSRITAQRALRFFAYGIGVLDGRAMPASHSALLDWYQTLGLPVCAERAV
                     VRGADGLLAFFRDIGARRRELPYEIDGIVYKVDRLDQQQRLGFVARAPRFALAHKFPA
                     EEAMTSVQGIDVQVGRTGAITPVARLAPIFVGGVTVTNATLHNEDEVRRKDVKIGDTV
                     IVRRAGDVIPEVVSVVPELRPADARPFSMPKTCPVCGSPIVRPEDEAVARCSGGWIKC
                     AAQRKGGLQHFVSRRAMDVEGMGEQLIDQLVEKGIVTTAADLYKLGLTGLAELDRMAE
                     KSAQNVLNALEKSKSTTLARFIFALGIRHVGEATAKELARHFGKLDRLLDASEEQLLE
                     VADIGPVVARSIRDFLADPLNRELIEQLRAAGIHWEEADGAASAPKPLQGKTFVLTGT
                     LPTMTRDEAARKIEEAGAKVAGSVSKKTSFVVAGEEAGSKLAKAQELGVPVLDEAGLM
                     KLLEDLK"
     gene            3980..4855
                     /gene="galU"
                     /locus_tag="VM406_11315"
     CDS             3980..4855
                     /gene="galU"
                     /locus_tag="VM406_11315"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HVL76595.1"
                     /translation="MRKVTKAVFPVAGLGSRFLPATKAQPKEMLPVVDKPLIQYAVEE
                     AVAAGITDMVFITGRNKRAIEDHFDTAYELENELESAGKTALLDLVRNAIPKNINCIY
                     IRQSAPLGLGHAVLCARPVIGNDPFAVLLADDLMDVAPGVAPVLAQMTDIYEQEGSSL
                     LAVQDVPRAETKQYGIVSVSPYKDRLERVHGIVEKPAPEEAPSTLAVVGRYVLSNRIF
                     EYLENLGQGAGGEIQLTDGIAQLMDAERVLAYRYDGRRFDCGSKLGYLQASVEIGKKH
                     PQIGAAFAEYLKQSA"
     gene            4852..5388
                     /gene="def"
                     /locus_tag="VM406_11320"
     CDS             4852..5388
                     /gene="def"
                     /locus_tag="VM406_11320"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HVL76596.1"
                     /translation="MSVRTILKMGDARLLREAEPVTDFDTPELHALVADMFETMRAVN
                     GAGLAAPQIGVNLRLVIFGFGRNARYPDAPPVPETVLINPVLTPLSEEMEEGWEGCLS
                     VPGLRGVVPRYRRLRYTGHDQYGQPIDREAQGFHARVVQHECDHLNGILYPMRIKDFS
                     QFGYTEVLFPGLDAAGDD"
     gene            complement(5392..6138)
                     /locus_tag="VM406_11325"
     CDS             complement(5392..6138)
                     /locus_tag="VM406_11325"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HVL76597.1"
                     /translation="MNEPIRLSKRMSELGLCSRREADEWIARGWVRVDGAVVSELGSK
                     VLPHQRISIERQASAEQARRVTILLNKPVGYVSGQAEDGYKPAVTLISAASRWEEDKT
                     PLQFHSSQLRSLVPAGRLDIDSVGLLVLTQDGRIAKQLIGEDTSVEKEYLVRVRYTKP
                     GRLPDSDLALLNHGLALDGRKLLPAKVSWQNEDQLRFILREGKKRQIRRMCEAVGLKV
                     LGLKRVRIGGVKLGKLPPGRWRYLAPDERF"
     gene            complement(6141..8693)
                     /locus_tag="VM406_11330"
     CDS             complement(6141..8693)
                     /locus_tag="VM406_11330"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HVL76598.1"
                     /translation="MMSLAATLKEQLRSEHQAAIDAFRTDARPDRLLAALRRNVDKAL
                     VRAWRELSMPADTALVAVGGYGRGELYPYSDVDVLILLPGQPDKARRERLETLVQLFW
                     DLGLEIGHSIRTIDECMSESAADITVQTSLLEARLITGNRRLFRALRERFNSAMDAQS
                     YFQAKMLELRQRHAKYEDTPYSLEPNVKESPGGLRDLQVILWVAKAAGLGDSWRKLAS
                     RGLLTATEARQLTQKERAFKDIRIRLHIHAGRREDRLVFDVQTPIAQTFGFQTTPTRR
                     ASEYLMQRYYWAAKAVTQLNAILLQNIQTQLFPHTGAPQPVNARFNQIDGLIDIARDD
                     TFETTPSAMLEVFLLIAQHQEIRGMTARTLRALWHARFRIDAAFRRDPHNRALFLEII
                     KAPQGITHALRGMNQTSVLGRYLPNFRRIIGQMQHDLFHVYTVDQHILMVVRNVRRFT
                     MTEHAHEYPFCSQLMANFAHPWLLYIAALFHDIAKGRGGDHSLLGMADARKFCVEHGL
                     TTEQTELVVFLVEHHLTMSLVAQKQDLSDPDVIRHFAAIVRDERHLTALYLLTVADIR
                     GTSPKVWNAWKGKLLEDLYRITLRVLGGEDPNAEHELRSRQQEALATLRLYGLPSSAH
                     QRLWQHLDVAYFLRHDAGDIAWQTRALWDKVDSKRAVVKCRLAPIGEGLQVAVYLRDQ
                     PDLFARICSYFDHKNFSILDAKIHTTRHGYALDTFLVSDPLFANSYRDIINLIEHELT
                     ELLESNQPLPPPSKGRLSRLSRTFPITPTVDLRPDERGQYYLLSVSANDRNGLLYSIA
                     TVLAKYRVNLHTAKIMTLGERVEDVFLVDGPVLSNARSQIQLETDLLDALRV"
     gene            complement(8706..9536)
                     /gene="map"
                     /locus_tag="VM406_11335"
     CDS             complement(8706..9536)
                     /gene="map"
                     /locus_tag="VM406_11335"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HVL76599.1"
                     /translation="MGITIKTEEDIKGMRIAGRLAAEVLDYITPFVKPGVTTGELDRL
                     CHQYMTEVQGSIPAPLNYSPPGYTPYPKSICTSVNDVVCHGIPGDKALKNGDVVNIDI
                     TVIKDGYHGDTSRMFYVGEPSILARRLTEVTFECMWIGISKIKPGAYLGDIGYAIQQH
                     AERAGYSVVREFCGHGIGKVFHEEPQVLHYGRPGTLERLVPGMIFTVEPMINAGRREI
                     REMGDGWTIKTKDRSLSAQWEHTVLVTETGYEVLTVSSGMPAPPAFIQQQTVVQPTSA
                     "
     gene            9790..10539
                     /gene="rpsB"
                     /locus_tag="VM406_11340"
     CDS             9790..10539
                     /gene="rpsB"
                     /locus_tag="VM406_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HVL76600.1"
                     /translation="MPVTMREMLEAGVHFGHQTRFWNPKMAPFIFGHRNKIHIVNLEK
                     TMAMYQEAMKYIRQLASNRGTILFVGTKRQARDILAAEAQRAGMPYVDQRWLGGMLTN
                     FKTIKTSIKRLKDMEAAIEDGSVDKMSKKEALMFQREMEKLQKSIGGIKDMGGIPDAI
                     FVIDVGYHKGAVTEAAKLGIPVIGVVDTNHSPDGVAYVIPGNDDSAKAITLYARGVAD
                     AILEGRANATNEVLEAVKGGADEFVEVSEQA"
     gene            10674..>11042
                     /gene="tsf"
                     /locus_tag="VM406_11345"
     CDS             10674..>11042
                     /gene="tsf"
                     /locus_tag="VM406_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HVL76601.1"
                     /translation="MAAITAAMVGELRAKTDAPMMECKKALTEADGDMARAEEILRVK
                     LGSKASKAASRVTAEGVVAASISGTVGALVEVNCETDFVTKNEDFIALANTAARLIAE
                     KNPVDVAALAALDAGGKTLDE"
BASE COUNT         2022 a         3388 c         3591 g         2041 t
ORIGIN      
        1 acatggtcaa gcgcatgtcc tcgcagaccc agttcctgtt catttcgcat aacaagattg
       61 cgatggaaat ggcgcagcag ctgatcggag tgacgatgca ggagcagggt gtttcccgca
      121 tcgtcgcggt ggacatggaa tcggccgcga acttcgcgtc cgaggcgcag gcggcttaga
      181 ccgaccgggc ggagcgcgcc atcgcggcgc gtttctggta atacgcgatg ttcatctgcg
      241 cttcgcgcat gcgctgcagt tcgacgtccg cgcagatcag gtagtgacgt tcggcgcgct
      301 tgagccacca gttgtgcagc gagcgcagga tcggcgacac ggacagcatg gtgatgtcct
      361 ggaggccgac gtcgcgggga gcggaggtgg cggcccttgc gcgtgcgacg cgagcgtcga
      421 tgtcgcgcga gtgcgccttg ctggcaagtt gacgctcgat ctcctggtgc tccgcttcgc
      481 ttgcgaaact gcgtgcgagc actgcttgat tccggcggtt caattccatg cgattctccc
      541 tggatcgtag tcttgaagtg ttgcaacgat agagacggct ttgcaaacac ttgctattct
      601 tgagtccggg gcagcccctg tcttgatgat cgtcaacggc atttacgtgt cgaatgggca
      661 aggatcaagt ttccattgaa catgcatgcg cggtcctgcg cgccttgaca actttccaat
      721 gcaacactac cctgaacccc tttttttcaa gcggtttcct tacatgcacc gcaccccgca
      781 ggcactcgcc cgcgatgctt tttccgagat gcaattgact gaggtgcaag cggcatgact
      841 gatctccagg caagtctgct gatcatcggc ggcacgatcg tggtcggcgt catcagctac
      901 aacaagtggc aggaatacaa ggccaggaaa tcggtccaga aagcattctc ttccgaccac
      961 gatgatgtcc tcatggaatc ggatatcgcc ggtgcggaag cggaaacccg tcgcgaacca
     1021 tccctcgata tacctgcagc ccacgccggt cccgagccga tcgctgagga aacgaacgag
     1081 gcggcagcca gcgccaagcc ggcaatccag aaagaactgc cgatcgacga gctgatcgac
     1141 ggcgccgtcg agctgatcct cgaagcgccg gtgcgcggcg agaagctgtt gccgaagctg
     1201 cagactcttc gccatgtcgg cagcaagccg gtccatttcg tcggccagcg cgaggacggc
     1261 gtctgggaag agattgcggt gggcggcatt tactacgggc tttacgccgg catccagctc
     1321 gctggccgca acggcccctt gaacgagctc gaattctcgg agctgatcac gcgcttgcgg
     1381 cagatcagcg acgaaatcga tgcggaaccc gatgtgcccg acatgagcga cgtaatggcg
     1441 cgcgcccgtg ccctgcatca gttcgtgtcg gaatatgacg cgcagttgag catcagcgtg
     1501 cgttccaagg gcgcaccgtg ggccataaac acgctgctgg ccgcgctcga gcgccagggt
     1561 ttcgacctgc gtcccgatgg acgtctggtc atgccggacg gtgacggggg cgtgctgttc
     1621 acgctgtcga ccaacgtcac acttgccgat gaaaccacgc cccgcctgac actcctgctc
     1681 gacgtgccgc gggtcgcgcc gctgcgggat ggctttggcg cgatggttgc ctgcgcgcgc
     1741 atgctcgcca cccgtctgga tggcgtggtg gtcgacgaca gcgaccagcc tttgcttgaa
     1801 cctgcactgg ccgaaatctc ggagcaggta acggccttct acaccaacat ggaaagcgca
     1861 ggcgtgccgg ccggctccac ccgggccttg cgcctgttcg cctgaggcga cgcatgcagc
     1921 ctgatttttt cggcgggccc gtcccgccgc ctctccgtga tcccgaagcc gacggctggc
     1981 aggagcgcgc ggcctggctc aaggatgagc tgaaccgtca cagccacgcg tattacgtcc
     2041 tcgacaatcc gacgattccg gatgccgaat acgaccgcat gttccgcgaa ctggtcgaac
     2101 tcgagcaggc gcatccggac ctggtcacat cggactcgcc gacccagcgc gtcgggggta
     2161 agcccctgcc tcagttcgag caggtgcggc atgcagttcc gatgctgtcg ctgaacaatg
     2221 ccttcgacga cgacgaggtg agaggcttcg accgccgcgt ctgcgaggcg ctccaggcag
     2281 acaacgtcgc ctatgcgacc gaactgaagt tcgacggcct cgcggtcaac ctgcgctacg
     2341 agaacggtgt gcttgtgcag gcggcgacgc gcggcgacgg cacgaccggc gagaacgtca
     2401 ccgcaaacat tcggaccgtg cgcgcgatac cgctgcgtct gcacggcagg gacgtgccga
     2461 aggtgatcga cgtgcgcggc gaggtgctga tgttcaaggc cgacttcgcg cgcctgaacg
     2521 aacgccagcg cgcggccggc cagaaggaat tcgccaatcc tcgcaatgcc gccgccggca
     2581 gcctgcgcca gctcgactca cgcattaccg cccagcgcgc gctgcgtttt ttcgcatacg
     2641 ggatcggcgt gctggacggc cgcgcgatgc cggcctcgca ctcggcgctg ctcgactggt
     2701 accagaccct cggtttgccg gtgtgcgcgg agcgcgcggt ggtgcggggt gcggacgggc
     2761 tgctcgcgtt cttccgcgac atcggcgcac ggcggcgcga gctgccgtat gagatcgacg
     2821 gcattgtcta caaggtagac cggctcgacc agcagcagcg gctcggcttt gtggcacggg
     2881 cgccgcgttt tgcgttggca cacaaattcc ccgccgagga agcgatgacg tcagtgcagg
     2941 gcatcgacgt ccaggtcggg cgcaccggtg cgatcacgcc ggtcgcgcgt ctggcgccga
     3001 tcttcgtcgg cggcgtgacg gtcaccaatg ccaccttgca caatgaggac gaggtgcggc
     3061 gcaaggacgt gaagatcggc gatacggtga tcgtacgtcg cgccggcgac gtgattccgg
     3121 aagtcgtgtc ggttgtgccg gagctgcggc cggccgacgc ccgcccgttc agcatgccca
     3181 agacctgccc ggtatgcggt tcgccgatcg tgcgcccgga agacgaggcg gtggcgcgct
     3241 gctccggcgg ctggatcaag tgcgcggccc aacgcaaggg cggcttgcag cacttcgtct
     3301 cgcgccgggc gatggatgtg gaaggcatgg gcgagcagct gatcgaccag ctggtggaaa
     3361 agggcatcgt gaccacggcc gccgacctgt acaagctggg cctcacgggg cttgctgaac
     3421 tcgaccggat ggccgagaag tctgcgcaga atgtattgaa tgcactcgag aaatcgaagt
     3481 cgacgacact cgcgcgtttc attttcgctc ttggcatccg gcacgtcggc gaggccaccg
     3541 ccaaggaact ggcgcgtcac ttcggcaagc tcgaccgcct gctcgatgcg agcgaggaac
     3601 aattgctcga agtcgccgac atcggccccg tcgtcgcccg ttcgatccgc gactttctcg
     3661 ccgatccgtt gaaccgcgaa ttgatcgagc aattacgcgc cgcgggcata cactgggaag
     3721 aagccgacgg cgcggccagc gcgccgaagc cgctgcaggg caagaccttt gtgctgaccg
     3781 gcaccttgcc caccatgaca cgcgacgagg cggcacggaa aatcgaggag gccggcgcca
     3841 aggtcgccgg ctcggtatcg aagaagacca gttttgtcgt ggcaggggag gaggctggaa
     3901 gcaagctggc caaggcgcag gaactcggcg tgcccgtgct cgatgaagcc gggctgatga
     3961 agctattgga ggacctgaaa tgagaaaagt cacgaaagcc gttttccccg ttgcaggcct
     4021 gggcagccgc ttcctgccgg ccaccaaggc gcagccgaag gaaatgctgc cggtggtcga
     4081 caagccgctg atccagtacg ctgtcgagga agccgtcgcg gccgggatta ccgacatggt
     4141 cttcattacc ggtcgcaaca agcgcgcgat cgaggaccat ttcgataccg cctacgagct
     4201 ggagaacgaa ctcgaatccg ccggcaagac ggcgctgctc gacctggtgc gcaacgcgat
     4261 cccgaagaac atcaactgca tctatatccg ccagtccgcg ccgctcgggc tggggcacgc
     4321 ggtattgtgc gcgcgcccgg tgatcggcaa cgacccgttc gcggtgctgc tcgccgacga
     4381 cctgatggac gtggctcccg gcgtggctcc cgtgcttgcg cagatgaccg acatctacga
     4441 acaggaagga tcgagcctgt tggccgtgca ggacgtgccg cgcgcggaaa ccaagcagta
     4501 cggcatcgtc agcgtgagcc cctacaagga cagactcgaa cgcgtgcacg gcatcgtaga
     4561 gaagccggcg ccggaagagg cgccatccac cctggccgtg gtcggccgct acgtgctgtc
     4621 caaccggatc ttcgaatacc tggaaaacct ggggcagggt gccggcgggg aaatccagct
     4681 caccgacggt attgcgcagc tgatggacgc cgagcgggtg ctggcctatc gctacgacgg
     4741 gcgacgcttc gattgcggct ccaagctcgg ctacctgcaa gcgagcgtcg aaatcggcaa
     4801 gaagcacccg cagatcggtg ccgcctttgc cgagtatctg aagcaatccg catgagcgtg
     4861 cgcactatcc tcaagatggg cgacgcgcgc ttgctgcgcg aggccgaacc ggttaccgac
     4921 ttcgatacgc cggagctgca cgcgctcgtc gccgacatgt tcgagaccat gcgtgcagtc
     4981 aatggcgccg gactggccgc cccgcagatc ggcgttaatc tgcggctcgt gatcttcggc
     5041 ttcggtcgca atgcgcgcta tccggatgcg ccgcccgtgc ccgaaacggt gctgatcaat
     5101 cctgtcctga cgcccttgtc cgaggagatg gaagaggggt gggaaggctg cctgtccgtg
     5161 cccggcctgc gtggggtggt gccgcgctac aggcgcctgc gctataccgg acatgaccag
     5221 tacggccagc cgatcgatcg cgaggcgcaa ggctttcatg cgcgcgtggt gcagcacgaa
     5281 tgcgatcacc tgaacggcat tctttatccg atgcgcatca aggatttttc gcaattcggt
     5341 tacaccgagg ttctttttcc gggcctcgac gccgccggcg acgactagcg gctaaaaccg
     5401 ctcgtccggc gccagataac gccagcggcc aggcggcagt ttgccgagct tgacgccgcc
     5461 gatgcgcact cgcttcaggc cgagcacctt caggcccacc gcctcgcaca tccggcggat
     5521 ctggcgcttc ttgccctcgc gcaggatgaa ccgcagctgg tcctcgttct gccacgaaac
     5581 cttcgccggc agcagtttcc tgccgtccag ggccagtccg tgattcaaga gcgcaagatc
     5641 cgagtccggc agccggccgg gcttcgtgta ccggacgcgc actagatatt ccttttccac
     5701 cgatgtgtct tcgccgatca gctgtttggc gatgcggccg tcctgcgtga gcacgagcag
     5761 gccaacggag tcgatatcga gccggccggc gggaaccagg ctgcgcagct ggctcgaatg
     5821 gaattgcagc ggtgtcttgt cttcttccca gcgggaggcc gcgctgatca acgtcacggc
     5881 cggcttgtag ccatcctcgg cctgcccgct gacgtagccg accggcttgt tgagcaggat
     5941 cgtgacccgg cgcgcctgtt cggcgctggc ctggcgctcg atcgagatgc gctgatgggg
     6001 caagaccttg ctgcccaact cggagaccac ggcgccatcg acgcgcaccc agccgcgcgc
     6061 gatccattcg tcggcttcac gccgcgagca cagtccgagt tcggacatgc gcttggaaag
     6121 tcgtatcggt tcattcatgg tcaaactcgc agcgcatcga gcagatcggt ttcgagctgg
     6181 atctggctgc gcgcattgct gagcaccggc ccgtctacca ggaagacgtc ctcgacccgt
     6241 tcgcccagcg tcatgatctt cgcggtatgc aggttcacac ggtacttcgc aagcacggtg
     6301 gcgatcgaat agagcaagcc attgcgatcg ttggccgata ccgacagaag gtaatactgg
     6361 ccgcgctcgt ccggccgcag atccacggtc ggggtgatcg ggaacgtgcg agaaaggcgc
     6421 gacaggcgtc ctttcgacgg aggcggcagc ggctggttcg attcgagcaa ttcggtcagt
     6481 tcgtgttcga tcaggttgat gatgtcgcgg tagctgttcg cgaacaacgg gtcgctgacc
     6541 aggaaggtgt cgagcgcgta gccgtgtcgc gtggtgtgga tttttgcgtc gaggatgctg
     6601 aagttcttat ggtcgaagta actgcagatg cgagcgaaca ggtcgggctg gtcgcgcagg
     6661 tagactgcca cctgcagccc ctcgccgatc ggcgcgagcc ggcacttcac aacggcgcgc
     6721 ttgctgtcga ccttgtccca cagcgcccgc gtctgccacg cgatgtcacc cgcgtcatgt
     6781 cgcaggaagt acgccacgtc caggtgttgc cacagcctct ggtgcgcgct cgacggcagc
     6841 ccgtacagcc gcagcgtcgc gagcgcctcc tgctggcggc tgcgcagttc gtgctcggcg
     6901 ttcgggtcct cgccgccgag gacgcgcagc gtgatgcggt acaggtcttc gagcagcttg
     6961 cctttccagg cattccagac tttcgggctg gttccgcgta tgtctgcaac ggtaagcagg
     7021 taaagcgcgg tcaggtgacg ctcgtcgcgt acgatggcgg cgaaatggcg gatgacgtcg
     7081 ggatccgaca ggtcctgttt ctgggccacc agggacatcg tcagatggtg ctcgacgagg
     7141 aagacgacga gctcggtctg ctccgttgtg agcccgtgct cgacacagaa cttgcgcgca
     7201 tcggccatgc ccagcagcga gtggtcgccg ccgcgtccct tggcgatgtc atggaacagc
     7261 gcggcgatgt acagcagcca gggatgggcg aagttcgcca tcagctggct gcagaacgga
     7321 tactcgtgtg catgctcggt catcgtgaag cgccgcacat tgcgcaccac catcaggatg
     7381 tgctggtcga cggtgtacac gtggaacagg tcgtgctgca tctgtccgat gatgcgccgg
     7441 aagttcggca ggtagcgccc gagcacgctg gtctggttca tgccgcgcag cgcgtgcgtg
     7501 atgccctgcg gtgccttgat gatctcgaga aacagtgcgc gattgtgcgg gtcccggcgg
     7561 aatgccgcat cgatgcgaaa gcgcgcatgc cacaacgcgc gcagggttcg cgccgtcatg
     7621 ccgcggattt cctgatgctg ggcgatcagc aggaacacct cgagcatcgc cgacggggtg
     7681 gtctcgaagg tgtcgtcacg ggcgatgtcg atcaggccgt cgatctggtt gaagcgcgcg
     7741 ttcactggct gcggcgctcc ggtgtgggga aacagctgcg tctggatgtt ctgcagcagg
     7801 atggcattga gctgggtgac cgccttggcc gcccagtagt agcgctgcat caggtactcg
     7861 ctggcgcgcc gggtgggcgt ggtctggaag ccgaacgttt gcgcgatcgg cgtctggaca
     7921 tcgaacacaa ggcggtcttc gcgtcgacct gcatggatgt gcaggcggat acgaatgtcc
     7981 ttgaatgcgc gctccttctg cgtgagctgg cgtgcctcgg tcgccgtgag caagccgcgg
     8041 ctggcgagct tgcgccagga gtcgcccagg cccgcggcct tggcgaccca caggatcacc
     8101 tgcaggtcac gcaagccgcc agggctctcc ttgacgttgg gttcgagact gtagggcgtg
     8161 tcctcgtact ttgcgtgacg ctggcgcagc tcgagcatct tggcctggaa atacgactgc
     8221 gcatccattg cggaattgaa gcgctcgcgc aatgcgcgga acagccggcg gttccccgtg
     8281 atcaatcgcg cttcgagcag gctggtctgg acggtgatgt cggccgccga ttcgctcatg
     8341 cattcgtcga tggtgcgaat actgtggcca atctccagtc cgaggtccca gaacagctgg
     8401 accagggttt cgaggcgctc gcgccgtgcc ttgtccggct gccctggcag caggatcaga
     8461 acgtcaacgt cggaatacgg atagagttcg ccgcggccat agccgcccac cgcgaccagg
     8521 gccgtgtcgg caggcatgct cagttcgcgc caggcacgga ccagcgcttt gtcgacattg
     8581 cgccgcagcg cggccaggag cctgtccggc cgggcatcgg tgcgaaaggc atcgattgcc
     8641 gcctggtgct cgctcctcaa ctgctccttg agcgtcgcgg cgagggacat catgttggta
     8701 gagtttcagg ccgaggtggg ctgcacgacg gtttgctgct ggatgaaagc gggcggcgcc
     8761 ggcatgccag acgagaccgt gaggacttcg tagccagtct cggtgaccag caccgtgtgc
     8821 tcccactgcg ccgacaggct gcgatccttg gtcttgatgg tccagccgtc gcccatttcg
     8881 cggatctcgc ggcgcccggc attgatcatc ggttcgaccg tgaagatcat gcctggcacc
     8941 agccgttcga gcgtcccggg tcggccgtag tgcagtacct gcggctcctc gtgaaatacc
     9001 ttgccgatgc cgtggccgca gaattcgcgc accacgctgt accctgcgcg ttcggcatgc
     9061 tgctggatcg cgtagccaat atcgcccaga tacgcgccgg gcttgatctt cgagatgccg
     9121 atccacatgc attcgaaagt cacctcggtc aggcgtctgg cgagaatcga cggctctccg
     9181 acatagaaca tccggctggt gtcgccgtga tatccgtcct tgatcaccgt gatatcgata
     9241 ttgaccacgt cgccgttctt cagtgccttg tcgccgggaa tgccgtggca gaccacatcg
     9301 ttgactgaag tgcagatcga cttcggatac ggggtgtagc cgggagggct gtagttcagg
     9361 ggggccggga tgctgccctg gacttcggtc atgtactggt ggcacagacg gtccagttcg
     9421 ccggtcgtca cgcccggttt gacgaacggg gtgatgtagt cgagcacttc cgcggccagc
     9481 cgcccggcga tgcgcatgcc cttgatgtct tcttccgtct tgattgtgat gcccatagat
     9541 tcctgcgccc tgcagcgatg ctgcaaacat tgcgttcaag ttgcaattat agtcgagccg
     9601 ccccagcgac ggctggaagc gctcgtcgag cttgaacggg aggatggttc tggagtagaa
     9661 tcttgggtta gcttgaaatg gtttcaagtt aattttttgt caaaatcgcg aacccggtcg
     9721 aaaaagggtg cccgtgcggt aagccggcgg gtgactgatc cggttcaaga cccaaccctg
     9781 gagatattca tgcccgttac catgcgcgaa atgctggaag ccggtgtcca tttcggccac
     9841 cagacccgct tctggaatcc caagatggcc ccgttcattt tcggccatcg caacaagatt
     9901 cacatcgtca acctggaaaa gactatggcg atgtaccagg aggcgatgaa gtacatccgc
     9961 caactggctt ccaaccgcgg caccatcctg ttcgtcggca ccaagcgcca ggcgcgtgac
    10021 atcctcgccg ctgaagccca gcgcgccggc atgccttacg ttgaccagcg ctggctgggc
    10081 ggcatgctca ccaacttcaa gaccatcaag acttccatca agcgcctgaa ggacatggaa
    10141 gcggcgatcg aagacggttc tgtcgacaag atgagcaaga aggaagcgct gatgttccag
    10201 cgcgagatgg agaagctgca gaagtccatc ggcggcatca aggacatggg tggtatcccg
    10261 gacgcgatct tcgtgatcga cgtcggctac cacaagggtg ccgtcacgga ggcagccaag
    10321 ctcggcattc cggtcatcgg cgtggtagat accaaccact cgccggatgg cgtggcctat
    10381 gtgatcccgg ggaacgacga ctccgccaag gcgatcaccc tgtacgcccg cggcgtcgcg
    10441 gatgcaatcc tcgaaggccg ggccaacgcg accaatgaag tgctggaagc cgtcaagggc
    10501 ggcgccgacg aattcgtcga ggtcagcgag caggcctgag ccttcgcttg cgcccagttg
    10561 tgacgtcggg cagaaaaggg ggcaacgctg gttcgcccct tttttttagc cggatgaacg
    10621 tatcttgatc gtacgcgtcc gaacggacgc tcaatgtatt ttaggagatt gacatggcgg
    10681 caattaccgc agcgatggtt ggtgaactgc gcgcgaagac cgacgcgccg atgatggagt
    10741 gcaagaaggc cctgaccgag gcggacggcg acatggcgcg cgccgaggaa atcctgcgcg
    10801 tcaagctcgg cagcaaggct tcgaaggcgg cttcccgcgt caccgccgaa ggcgtggtcg
    10861 cggcctctat ttccggcacc gtcggcgcgc tggtcgaagt caactgcgaa accgatttcg
    10921 tgaccaagaa cgaggatttc atcgcgctgg ccaataccgc cgcccgcctg atcgccgaaa
    10981 agaatccagt cgacgtggcc gcactggcgg cactggacgc gggcggcaag acgctcgatg
    11041 aa
//
LOCUS       DATLGF010000368        11255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_171754, whole genome shotgun sequence.
ACCESSION   DATLGF010000368 DATLGF010000000
VERSION     DATLGF010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 11255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11255
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171754"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VM406_11350"
     CDS             complement(<1..477)
                     /locus_tag="VM406_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HVL76602.1"
                     /translation="MMNTLTGMRVAVLVTDGFEQVEMTGPIQALERADASVQIVSDHT
                     GEVRGCHGEQPADTFEVHFRFADADPMDFDAVLLPGGPKNGERIATIPEAQHFVQQMD
                     ELGKPIAAICHGPLLLASAGLLEGRTMTSWPEIESDMRQVGANWVDREVVVDGNLIT"
     gene            638..3052
                     /gene="lon"
                     /locus_tag="VM406_11355"
     CDS             638..3052
                     /gene="lon"
                     /locus_tag="VM406_11355"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HVL76603.1"
                     /translation="MKAEHNPSQGASLSVSPSSSAAIQFTPGVLPRAPLPEDALPIIP
                     MRQLVLFPGAIAPISLVRAGSIAAANEAVQHDRPVGLLLQRDPDMDVPGARDLYTTGT
                     VANVLRHITSDEGESYLVCQGVRRFRVLDFLDGFPFHAARVEEFPDQEEEAPDIQARM
                     LQLKERSAEVIGLLPQAPSELAGAADSIDSAAALSDFIVGLIDLPLEQKQQVLELSDL
                     RQRLDVVLGLLIRRIEVLRISQEINEQTKERIDDRQREFLLREQMRTIRDELGEGNGP
                     ESVAQELGEAIAKAGMPDDVLAHVRKELQRLERMPEISAEYSMLCTYLEAMSELPWSR
                     LSEDRLDIAEARRILDEDHFGLQMVKRRILEHLAVRKLNPAGKSPILCFVGPPGVGKT
                     SLGQSIARATGRKFARVSLGGVHDEAEIRGHRRTYVGAMPGNIIQAVRKAGTRNCVLM
                     LDEMDKLGTGAHGDPSAALLEVLDPAQNATFRDNYVGQPFDLSQVMFIGTANMLDAIP
                     GPLLDRMEVIRLPGYTVEEKVQIAHRYLLARERATAGLREEQCRISEEAVRIIISDYT
                     REAGVRNLERELGNVFRHAAVRVAEGTDEQVQVAPEDLPGILGPRKFESELALRTSVC
                     GVATGLAWTPVGGDILFIEASCAPGSGKLILTGQLGDVMKESAQAALTLLKSRATELG
                     VDPAVFDRSDIHVHVPAGAMPKDGPSAGVAIHVALASLLSEIPVRHDCAMTGEISLRG
                     LILPVGGVKEKVLAALSAGIRTVLLPARNRKDLDDIPAEARDALRFIWLDTADQALAA
                     SLSEKR"
     gene            3056..3415
                     /locus_tag="VM406_11360"
     CDS             3056..3415
                     /locus_tag="VM406_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76604.1"
                     /translation="MSKELEKASRAPVPFDAGRALHDTLNDGLAQQPPPGFVRFRYTC
                     TEIHSHGGQVDVRMRQTRYEDGHLTTEECEGTMDRDAFRREVQARQVEAIENVFGLAR
                     LLLAPLFGSARGSRRSR"
     gene            complement(3495..4253)
                     /locus_tag="VM406_11365"
     CDS             complement(3495..4253)
                     /locus_tag="VM406_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HVL76605.1"
                     /translation="MNPNPDQIEQFNRDGYLICRGLASQADCAAMLAVTRVHLEAAIE
                     PLEYEADLGYPGAPGSRDAAGGSTIRRLRGAWQRDPVFRAWASDPRIVNWLERRFGEP
                     VCVTLAHHNCVMTKHPAYGTATGWHRDIRYWAFARPDLVAVWLALGPEGPSNGGLSVI
                     PGSHRMDIRREQLDALDFLRPDVAENRALMKHGVALELEAGDVVFFHSGLFHAAGRNA
                     SDQVKCSVVFAYRAASNPPVTGSRSAAGDGGVCF"
     gene            4373..4969
                     /locus_tag="VM406_11370"
     CDS             4373..4969
                     /locus_tag="VM406_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76606.1"
                     /translation="MRRLAALGIGAAAPPMLLRAAMAADPRAGLLRAEGEVLVNGRPA
                     HPGAAIGPGSRVETGPGALAVFVAGGDALLLRAQSRIEIDEEHGLRVLRHARGAVLSV
                     FGGGRRAIVTPTASIGVQDASCYLESSPERTTFCLCRGHALVVPKADPNRRKSLRTLH
                     HEHPVHIERAAGARAVVPAKFIHHSDAELAMLEALLGR"
     gene            complement(4971..6065)
                     /locus_tag="VM406_11375"
     CDS             complement(4971..6065)
                     /locus_tag="VM406_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HVL76607.1"
                     /translation="MSRTARRLLIAAAVLAALVIAVVIGFRIAVDTLKTRIEQALGPE
                     SEVKMLVIGWTAIELHGIRIRAPKGWPAEDALRAERVTITPDFAGLFSARVHVPRIVV
                     EQPYLSVHRTANGRMRLLPSLLERNATPAGGAGPAVPVTIGEVVLQDGVLEFFDASVR
                     RPAHRTRLERLQAKVQDIAVPALDGRTTLQLDGVIKGTRRDGTLAVSGWAELAARNSD
                     IRTKLAGVDLVALQPYLIKTNEAGVRRGTLDLSLHSKVRNNMLHAPGKAAIADLELSA
                     GGGTFSTFMGIPRQAVIGAMKNRDGRITVDFTLEGNLDDPKFSLNENFALRMGAGVAQ
                     GLGISLEGLAKGVGGAAEGIGSAFKKLFGQ"
     gene            6293..7396
                     /locus_tag="VM406_11380"
     CDS             6293..7396
                     /locus_tag="VM406_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HVL76608.1"
                     /translation="MARTSDNSASSVPVPGLRHVSDARPGITREAAGKGFRYRHPDGS
                     LVEDEATLARIKALAVPPAWSEVWICPHANGHIQAIGRDARGRKQYRYHPRWRAVRDE
                     AKYERMLGFAKALPQIRRHVDADMKRPGLPREKVLATIVHLLETTMMRIGNEEYAREN
                     KSFGLTTLRDRHAKIEGSAVEFRFRGKSGVMHAVKVGDPRVARIVRRARDLPGQELFQ
                     YVDDDGELRVVDSADVNAYLREITGEDYTAKDFRTWSGTLLAALALQEYEKFDSDAQA
                     KKNVVRAIESVAARLGNTPSICRKCYVHPVVIESYLDGTMLHALRERAREELAEDLHA
                     LRPEEAAVLALLQRRLADAETRARTRSGARRAG"
     gene            7508..8083
                     /locus_tag="VM406_11385"
     CDS             7508..8083
                     /locus_tag="VM406_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76609.1"
                     /translation="MTLAADALTMTLLVLLAVAFVAALAYALRLRGRARQGERESQAI
                     AATLVEYFRRSGVTVATDCVRGADGRYTALIESEPMKRFRLSHIIEMTLRDHVRKATG
                     LELDRIYWRFPVKEAVAAAAAPAAAAPAEAQAPAPDAASTDATMELSDDYINEGLEHY
                     RHIPKPEVDELPWEAFEEVTGRGQAKEPPAG"
     gene            8324..9409
                     /locus_tag="VM406_11390"
     CDS             8324..9409
                     /locus_tag="VM406_11390"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVL76610.1"
                     /translation="MQEQQGSTPRPGQRVPPPDDVPMERASSEQGGALTATEIRRDDD
                     SAIPGTQGPREGAYHYARPQARRGMDSAGIVQALGWFSVGLGLAQLLAPRQMSRAIGA
                     GDHPHMMRACGTRELASGIGILTQRRPATWLWSRVAGDALDLFLLGAAARSPHARRTR
                     IGMVGAAVAGIAVLDLLTSMKAPEHQALGEEVEFEKTIVVNRSPEECYRYWRDLEGLP
                     RFMHHLREVHVIDEKRSLWRAKGPLGFSIEWHSEITDDEPNEYIAWRSEAGSDIDTEG
                     SVRFTPAPSGRGTLVYTHMRYRPPGGAAGYIVSKLFGDEPSQQIDEELRRFKWLLETG
                     EIPTTVGQPAGQRGAWNRYVIRKGAAG"
     gene            9406..10575
                     /locus_tag="VM406_11395"
     CDS             9406..10575
                     /locus_tag="VM406_11395"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HVL76611.1"
                     /translation="MRANVWHGKRDVRVDTVPDPQILNPGDAIIRITSTAICGSDLHL
                     YNGYVPTMEKGDIIGHEFMGEVVEVGSEVKKLKIGDRVVVPFPIACGHCFFCEEELYS
                     VCENSNPNAWMAEQLWGHSPAGIFGYSHLTGGYAGGQAEYARVPFADVGPIKIPDGLT
                     DDQVLFLSDILPTGYMAAEACEIKPGATVAVWGCGPVGQFCIQSAYLLGAERVIAIDR
                     FPERLRMAREKSKAITLDYTQVNVLEALKEMTGGRGPDACIDAVGLEAHWSGPLGAVD
                     RAKQAMMLETGRPVALREAIMACRNGGVVSIPGVYGGFLDTMPMGSIMNRSLTIKTGQ
                     THVHRYLQPLLERIQKGEIDPTMVITHHMSLEDAPRGFEMFSNKQDECIKIVLKP"
     gene            complement(10653..10952)
                     /locus_tag="VM406_11400"
     CDS             complement(10653..10952)
                     /locus_tag="VM406_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76612.1"
                     /translation="MDHLLTLLYGASGITAAALYLPQIRTYHRDPAARSSISLLTWGG
                     WIAIACITILYAALVVDNALIATIAALNLLAQIAVLGYGISARRRPARAVADPGL"
BASE COUNT         1863 a         3716 c         3860 g         1816 t
ORIGIN      
        1 ggtgatcaga ttgccgtcca ccaccacttc gcggtcgacc cagttggcgc ccacctggcg
       61 catgtcggat tcgatttcgg gccagctcgt cattgtgcgc ccttccagca ggccggccga
      121 ggccaacagg agcggaccgt ggcagattgc cgcgatcggc ttgccgagct cgtccatctg
      181 ctgcacgaag tgctgggctt ccgggatggt ggcgatgcgt tcgccgttct tcgggccgcc
      241 cggcagcagc accgcatcga aatccatcgg atcggcgtcg gcaaagcgga aatgcacttc
      301 gaacgtgtcc gcgggctgct cgccgtggca gccgcgcacc tccccggtat ggtcgctgac
      361 gatctgcacg gaagcatccg cccgctccag tgcctgaatg gggccggtca tctccacctg
      421 ttcgaagccg tccgtcacga gaacggcgac gcgcatgccg gtcagggtgt tcatcatcgc
      481 gcttcctcct tcgcaagttc cgacggttcg actgaccgat gccgggcgag ttcggcgcag
      541 gggctttgtc cgcctgcaag cactggccgg ctgccgggat gatctaggcg aaagccgggc
      601 ccggaacgcg ggttgtgcgg gcatgtcgaa cagtgtcatg aaagcagaac acaatccgag
      661 ccagggggcc tcactctccg tgtcgccttc ttcgtcggcg gcgatccagt tcacgccggg
      721 cgtgctgccg cgcgcgccgc tgccggagga tgcgctgccg atcatcccga tgcgccagct
      781 ggtgctgttt ccgggcgcta tcgcgccgat cagccttgtg cgagccggct cgatcgccgc
      841 cgccaatgag gcggtgcagc acgatcggcc ggtcggcttg ctgctgcagc gcgatccgga
      901 catggacgtt ccgggcgcgc gcgatctcta cacgacgggc acggtggcga acgtcttgcg
      961 ccacatcacc tccgacgagg gcgagagcta cctggtctgc cagggcgtgc gccgcttccg
     1021 cgtgctcgac ttcctggacg gctttccgtt ccacgctgcg cgggtcgagg aatttcccga
     1081 tcaggaggag gaggcgccgg atatccaggc gcgcatgctg caactgaagg agcgctccgc
     1141 cgaagtgatc ggcctgctgc cgcaggcgcc gtcggaactg gccggcgccg ccgacagcat
     1201 cgactcggcc gccgcgttgt ctgatttcat cgtcggattg atcgatcttc cgctcgagca
     1261 gaagcagcag gtgctggagc tttcggacct gcgccagcgc ctggacgtgg tgctgggcct
     1321 gctgatccgc cgcatcgagg tgctgcggat ctcgcaggaa atcaacgaac agaccaagga
     1381 acgcatcgac gaccgtcagc gcgagttctt gctgcgagaa cagatgcgca cgattcgcga
     1441 cgagcttggc gagggcaacg gtccggagag cgtcgcgcag gaactcggcg aggccatcgc
     1501 caaggccggc atgccggacg acgtgctcgc gcatgtgcgc aaggagctgc agcggctgga
     1561 gcgcatgccc gaaatttcgg ccgagtactc gatgctgtgc acctacctgg aagcgatgag
     1621 cgagctgccg tggtcgcgcc tgagcgaaga ccggctcgac atcgccgagg cgcgccgcat
     1681 tcttgacgag gaccatttcg gcttgcagat ggtcaagcgg cgcatcctcg aacacctggc
     1741 cgtacgcaag ctcaacccgg ccggcaagag cccgatcctg tgcttcgtgg ggccgcccgg
     1801 cgtcggcaag acctcgctcg ggcagagcat cgcgcgcgcg accggccgca agttcgcgcg
     1861 ggtttcgctg ggcggcgtgc atgacgaggc cgagatccgc ggccaccggc gcacctatgt
     1921 cggtgccatg cccgggaaca tcatccaggc ggtgcgcaag gccggcacgc gcaactgcgt
     1981 cctgatgctc gacgagatgg acaagctcgg caccggcgcc cacggcgatc cttcggccgc
     2041 gctgctcgaa gtgctcgatc cggcccagaa cgcgaccttc cgcgacaact acgtcggcca
     2101 gccgttcgac ctctcgcagg tgatgttcat cggcaccgcc aacatgctcg atgcgatacc
     2161 ggggcccttg ctcgaccgca tggaagtcat ccgcctgccc ggttatacgg tggaggagaa
     2221 ggtgcagatc gcacaccgtt acctgctcgc gcgcgaacgc gcgaccgccg ggctgcgcga
     2281 ggaacagtgc cggatctccg aagaggcggt gcgcatcatc atcagcgact acacgcgcga
     2341 ggccggcgtg cgcaatctcg aacgcgagct cggcaatgtg ttccgtcatg cggcggtgcg
     2401 cgtcgcggag gggacggacg agcaggtgca ggttgcgccg gaggacctgc ccggcattct
     2461 ggggccgcgc aagttcgaaa gcgagctggc actgcgcacc agcgtgtgcg gcgtggccac
     2521 cggcctggca tggacgccgg tcggcggcga catcctgttc atcgaagcct cgtgcgcgcc
     2581 cggcagcggc aagctgatcc tgaccgggca gctcggcgac gtgatgaagg aaagcgcgca
     2641 ggccgcgctg accctgctca agtcgcgcgc gacggaactg ggcgtggacc cggcggtctt
     2701 cgacaggagc gacatccacg tccacgtgcc tgccggcgcg atgccgaagg atggaccgag
     2761 cgccggcgtg gcgatccatg tggcgctggc gtcgctgctg tcggagattc cagtgcggca
     2821 cgactgcgcg atgaccggcg agatcagcct gcgcggcctg attcttccgg ttggtggcgt
     2881 gaaggaaaag gtgctcgcgg cgctgagcgc cggcatccgg accgtgctgc tgcccgcgcg
     2941 caaccgcaag gatctggacg acattccggc ggaagcaaga gacgcgctgc ggttcatctg
     3001 gctcgacacc gccgatcagg ccctggccgc gtcactgagc gaaaagaggt aagcgatgag
     3061 caaggaactg gaaaaggcga gccgtgctcc ggtcccgttc gacgccggac gtgcgctgca
     3121 tgacacgctg aacgacggcc tggcgcagca gcctcctccc ggctttgtgc gcttccgtta
     3181 cacctgtacc gagattcatt cccatggcgg ccaggtcgac gtgcgcatgc gccagacccg
     3241 ctacgaagac ggacacctga cgaccgagga atgcgaaggg acgatggacc gggatgcgtt
     3301 ccggcgcgaa gtgcaggcgc gccaggtgga ggcaatcgaa aacgtattcg ggctggccag
     3361 gctgctgctg gcgccgctgt tcggttccgc gcgcggctcg cgccggtcgc gttgacgggc
     3421 gggttcaagg gtcgtcatcg ccttgcgcac cggagatcct tcgctgcgct caggatgaca
     3481 gagagtagaa cgcctcagaa acacaccccg ccgtctcccg ccgccgagcg cgagcccgtt
     3541 accggcgggt tgctggccgc gcggtaggcg aacaccacgg agcacttcac ctggtcgctg
     3601 gcattgcggc cggccgcatg gaacaggccg ctgtgaaaga acaccacgtc gccggcctcc
     3661 agttcgagcg cgacgccgtg cttcatcagc gcgcggttct ccgccacgtc cggacgcagg
     3721 aaatccagcg catcgagctg ttcgcggcgg atgtccatcc ggtgcgagcc gggtatgacg
     3781 gacagcccgc cgttggacgg cccctccggt ccgagcgcaa gccacaccgc aaccagatcg
     3841 ggccgcgcga acgcccagta gcggatatcg cgatgccagc cggtcgccgt gccgtaggcc
     3901 ggatgcttgg tcatcacgca gttgtggtgg gccagcgtga cacacaccgg ctcgccgaag
     3961 cgccgctcga gccagttgac gatgcgcgga tccgaggccc aggcgcggaa taccggatcg
     4021 cgctgccagg cgccgcgcag gcgccgtatc gtgctgccgc cggcggcgtc ccgcgaaccg
     4081 ggcgcgcccg gatagccgag atccgcctcg tattcaaggg gctcgatcgc ggcctcgaga
     4141 tgcacgcggg tgacggcgag catggccgcg cagtcggcct gcgaggccag gccgcggcag
     4201 atcaggtagc cgtcgcggtt gaattgttcg atctggtcgg ggttcggatt catggcgttg
     4261 gtcaagtata cgtcagcgca ttgtccgccg gccggcgcta tgcttgaccc atggagcaag
     4321 ccgggaagag gagatgacac gggaagcgcc cgcatggaca cgccgtcgcg cgttgcggcg
     4381 tctcgccgcc ctcggcatcg gcgcggcggc gccgccgatg ctgctgcgcg ccgccatggc
     4441 cgccgatccg cgcgccggcc tgctccgggc cgagggcgag gtgctggtga acggtcgccc
     4501 ggcacatccc ggtgctgcga tcgggccggg ctcgcgcgtg gaaaccgggc cgggcgcgct
     4561 ggccgtgttc gtcgccggcg gggatgcctt gctgctgcgc gcgcagagcc ggatcgagat
     4621 cgacgaggag catggcttgc gcgtgctgcg tcatgcgcgg ggcgcggtgc tgagcgtgtt
     4681 cggaggcggg cggcgcgcga tcgttacgcc gacggcgagc atcggcgtgc aggacgccag
     4741 ctgctatctc gaaagcagtc ccgagcggac caccttctgt ctgtgccgcg gacacgcgct
     4801 ggtcgtgccg aaggccgatc cgaaccggcg caagtcgttg cgcaccctcc atcacgagca
     4861 tccggtgcac atcgagcgcg ctgccggcgc acgcgcagtg gtgcccgcaa agttcatcca
     4921 tcattccgac gcggaactgg caatgctcga ggcgctgctt ggccgctgag ctattggccg
     4981 aacagcttct tgaacgcgct gcctatgcct tcggcggcgc cgccgacgcc cttggccagc
     5041 ccctccaggc tgatgcccag tccctgcgcg acgccagcgc ccatgcgcag cgcgaagttc
     5101 tcgttcagcg agaacttcgg atcgtcgaga ttcccctcca gcgtgaaatc gacggtgatg
     5161 cgcccgtcgc ggttcttcat cgctccgatc accgcctggc gcggaatgcc catgaaggtc
     5221 gagaacgttc cgccgccggc gctcagttcg agatccgcga tggcggcctt gcccggggcg
     5281 tgcagcatgt tgttgcgtac cttcgaatgc agcgaaagat cgagcgtgcc gcgccgcacg
     5341 ccggcttcgt tggtcttgat caggtagggc tgcagcgcga cgaggtccac cccggcgagt
     5401 ttcgtgcgga tgtcggaatt gcgcgcggcc agttccgccc agccggagac ggccagcgtg
     5461 ccgtcgcggc gcgtgccctt gatcacgccg tcgagctgca atgtggtgcg gccgtcgagc
     5521 gcgggcacgg cgatatcctg caccttcgcc tgaaggcgct ccagccgggt gcggtgggcc
     5581 ggccggcgca cgctggcgtc gaagaattcc agcacgccgt cctgcagcac cacttcgccg
     5641 atggttaccg gaacggccgg gccggcgccg ccggcaggcg tcgcgttacg ttccagcagg
     5701 ctcggtagca ggcgcatgcg gccattcgca gtgcggtgca cggacagata cggttgttcg
     5761 accacgatgc gcggcacgtg cacgcgggct gagaacaggc cggcgaagtc gggggtgatc
     5821 gtgacccgtt cggcgcgcag cgcatcctcg gccggccagc ccttgggcgc ccgaatgcgg
     5881 atgccgtgca gctcgatcgc cgtccagccg atcaccagca tcttcacttc gctttccggg
     5941 cccagtgcct gctcgatccg ggtcttgagc gtatcgacgg cgatacggaa gccgatgacg
     6001 acggcgatga cgagggccgc gaggacggcg gcggcaataa gaaggcggcg ggcggtgcgg
     6061 ctcatgctcg gtgaattgct tgcttgcaac agatgattcc tgaacggtat cagcgcttgc
     6121 gccgaaaatc cactgcccgt gcatctgccg gccccggccg cgcacgccgc agcgacgcgt
     6181 ctcgttcgtc ctgctcttgt tcttgcccgc aaaagccggc ccgattgcgc gcgctcacgg
     6241 ggtttcccgg cggcggcgtt acagtggggt tattgctccc gcttgcctgc gcatggcccg
     6301 tacgtccgac aattccgctt ccagtgtccc ggtcccgggc ctgcgccacg ttagcgatgc
     6361 ccggcccggc atcacccgtg aagcggcggg aaaaggcttc cgctaccgtc atccggacgg
     6421 cagcctggtc gaggacgagg cgacgctggc gcgcatcaag gcactggccg taccgccggc
     6481 gtggagcgag gtatggatct gtccgcatgc gaacggccat atccaggcca tcggacgcga
     6541 tgcgcgcggg cgcaagcagt atcgctacca cccgcgctgg cgcgccgtgc gcgacgaggc
     6601 gaagtacgaa cgcatgctcg gctttgcgaa ggcgctgccg cagatccgcc ggcatgtcga
     6661 cgcagacatg aagcggccgg ggctgccgcg cgagaaggtg ctggcgacca tcgtccactt
     6721 gctggagacg acgatgatgc gcatcgggaa cgaggagtat gcgcgcgaaa acaagtcctt
     6781 cggcctgacc acgctgcgcg accgccacgc gaagatcgag gggagcgcgg tcgagttccg
     6841 ctttcgcggc aagagcggag tcatgcacgc ggtgaaggtc ggcgacccgc gcgtggcccg
     6901 gatcgtgcgg cgcgcccggg acttgccggg ccaggaactg ttccagtacg tggacgacga
     6961 cggcgagctg cgcgtggtcg actcggcaga tgtgaatgcc tacctgcgcg aaatcaccgg
     7021 cgaggactac accgccaagg atttccgcac ctggtcgggc accctgctgg cggcgctggc
     7081 gctgcaggag tatgaaaaat tcgattccga cgcgcaggcg aagaagaacg tggtgcgcgc
     7141 gatcgagtcg gtcgccgcgc ggctgggcaa tacgccgtcc atctgccgca agtgctacgt
     7201 gcatccggtg gtgatcgagt cctatctgga cggcacgatg ctgcatgcgc tgcgtgagcg
     7261 cgcgcgcgag gaactggcgg aagacctgca tgcgctgcgg ccggaggagg cggcggtgct
     7321 ggcgttgctg caacgccggc tggccgatgc cgaaacacgc gcacgcaccc gttccggggc
     7381 gcggcgcgcg ggctgaaatt ctgcttgccc tggagcaacg cgatctgtca agccaatgtt
     7441 atgatcctca cttgtttcga attctgcctg ccattccaca accgctcgcc agagaggttc
     7501 gacccgcatg acactcgctg ctgacgccct gacgatgacc ctgctggttc tcctggccgt
     7561 tgccttcgtt gcagcgcttg cctacgcgct gcgcctgcgc ggccgggcca ggcagggcga
     7621 gcgcgagtcc caggccattg ccgcgaccct ggtcgaatat ttccgccgca gcggcgtcac
     7681 cgtcgcgacc gattgcgtgc gcggcgccga cggccgctat accgcgctga tcgaatcgga
     7741 gccgatgaag cgcttccgtt tgtcgcacat catcgagatg acgctgcgcg accatgtgcg
     7801 caaggccacc ggcctcgaac tcgacaggat ctactggcgc ttcccggtca aggaagcggt
     7861 ggccgctgcg gctgccccgg cggcggccgc gccggccgag gcgcaggccc cggcgccgga
     7921 cgcggccagc accgatgcca caatggaatt gtccgacgac tacatcaatg aaggcctcga
     7981 gcactaccgt cacattccca agccggaagt cgacgagctc ccctgggaag ccttcgagga
     8041 agtaaccggt cgcggccagg ccaaggagcc gccggcaggc tgacccttcc gcagcgactc
     8101 ctttcacagc ggcccgcgtg ccgcaaccta cctctcccgc attcccccgg ccatccacct
     8161 gctgggaact cgccttctta acgtttgact aacagtcctc tatgggcgtg ccgcgattgc
     8221 gcgcgcgtcc cttcacgcaa caggaccgca gtgcccgggc gcatggccct tcgcagcacg
     8281 cgcagggaag tgcccgcctg catgtcgcca ggaggacagc aagatgcaag aacagcaggg
     8341 cagcactccc cggccgggac agcgcgtacc gccgccggat gacgtgccga tggaacgcgc
     8401 atccagcgaa cagggcggag cactcaccgc cactgaaatc aggcgggacg acgactccgc
     8461 catccccggc acccagggcc cgcgcgaggg cgcgtatcac tatgcgcggc cgcaggcgcg
     8521 ccgcgggatg gactcggccg gcatcgtgca ggcgctgggc tggttcagcg tcggcctcgg
     8581 cctggcccag ctgctggcgc cgcgccagat gtcgcgcgcg atcggcgccg gcgaccatcc
     8641 gcacatgatg cgggcctgcg gcacgcgcga actggccagc ggcatcggca tcctgaccca
     8701 gcggcgtccc gccacctggc tgtggtcgcg cgtggccggc gatgcgctcg acctgttcct
     8761 gctcggcgcg gccgcccgct cgccgcatgc ccgccgcacc cgcatcggga tggtcggcgc
     8821 ggcggtggcc ggcatcgcgg tgctcgatct tctgaccagc atgaaggcgc ccgagcacca
     8881 ggcgctcggc gaggaggtcg aattcgagaa gaccatcgtg gtcaaccgtt cgcccgagga
     8941 gtgctaccgc tactggcgcg acctggaggg tctgccgcgc ttcatgcacc acctgcgcga
     9001 agtgcatgtg atcgacgaga agcgcagcct gtggcgcgcc aaggggccgc tgggcttttc
     9061 gatcgagtgg cactccgaga tcaccgacga cgagccgaac gagtacatcg cctggcgctc
     9121 ggaggccgga tcggacatcg acaccgaggg cagcgtgcgc ttcacgccgg cgccatccgg
     9181 gcgcggcacc ctggtctaca cccacatgcg ctatcgcccg ccgggcggcg ccgcgggcta
     9241 catcgtcagc aagctgttcg gcgacgaacc ttcgcagcag atcgacgagg agctgcgccg
     9301 tttcaagtgg ctgctcgaaa ccggcgagat cccgaccacg gtcgggcaac ccgccggcca
     9361 gcgcggcgcg tggaatcgtt acgtgatcag gaaaggagcg gcgggatgag agcgaatgtc
     9421 tggcatggaa agcgcgacgt gcgggtggac accgtgcccg atccgcagat actcaacccc
     9481 ggcgacgcga tcatcaggat cacctcgacc gccatctgcg gctcggatct gcatctgtac
     9541 aacggctatg tgccgaccat ggaaaaaggc gacatcatcg gccatgagtt catgggcgag
     9601 gtggtcgagg tcggcagcga ggtgaagaag ctgaagatcg gcgaccgcgt ggtggtgccg
     9661 tttccgatcg cctgcggcca ctgcttcttc tgcgaggagg agctgtactc cgtgtgcgag
     9721 aactccaatc cgaatgcctg gatggctgaa cagctgtggg gacactcgcc ggccgggatc
     9781 ttcggctatt cgcacctgac cggcggctat gccggcggtc aggcggagta tgcgcgcgtg
     9841 cccttcgccg atgtgggtcc gatcaagatt cccgacgggc tgaccgacga ccaggtgctg
     9901 tttctgtccg atatcctgcc gaccggttac atggccgcgg aagcctgcga gatcaagccc
     9961 ggcgcgacag tcgcggtgtg gggctgcgga ccggtcggcc agttctgcat ccagagcgcc
    10021 tacctgctcg gcgccgagcg ggtgatcgcg atcgaccgct ttccggagcg gctgcgcatg
    10081 gcgcgcgaga agtccaaggc gatcacgctg gattacaccc aggtcaacgt gctcgaggcg
    10141 ctgaaggaga tgaccggcgg acgcgggccg gacgcctgca tcgatgcggt cggcctggag
    10201 gcgcactggt ccggtccgct tggcgcggtc gaccgcgcca agcaggcaat gatgctggag
    10261 accggccgcc cggtcgcgct gcgcgaagcc atcatggctt gccgcaacgg cggcgtggtg
    10321 tcgatcccgg gggtgtatgg cggcttcctc gacacgatgc ccatgggctc gatcatgaac
    10381 cggtcgctca ccatcaagac cggtcagacg catgtgcacc gctatctgca gcccttgctc
    10441 gaacgcatac agaaaggcga gatcgatccg accatggtga tcacccacca catgtcgctg
    10501 gaagacgcgc cgcgcggctt cgagatgttc tccaacaagc aggacgagtg catcaagatc
    10561 gtgctcaagc cgtgatcagc gcctgagctg ggccgcgcag cgctgtttgc tcgcggccca
    10621 tcgaatgatc cggccttgtg gagatgaacc ggttacaggc cgggatcggc gaccgctcgc
    10681 gccgggcgac gccgcgcgct gattccgtag cccagtaccg cgatctgggc aaggaggttg
    10741 agcgcggcga tcgtcgcgat cagcgcattg tccaccacca gcgcggcgta caggatcgtg
    10801 atgcaggcga tcgcaatcca cccgccccag gtcagcagcg agatcgagct gcgcgcggcg
    10861 gggtcgcggt gataggtgcg gatctgggga aggtagagcg ctgccgccgt gattccggag
    10921 gcgccgtaca gcagggtgag caagtggtcc atggggaggg cgagaggcgc acacagagcg
    10981 cgcatgcgcg ctcattctac ggcccaattt ttgccgtgca tcatcacccg acggaaatat
    11041 tttcaggctg ttgcgggcct gccatggttg catgcgttta acggaactcg gcgggacagg
    11101 ggctgtcgtt ttttcaagcc caatcgtttc ggaggaccct tccatgagag tactgctgtg
    11161 gatcatcgcg atcatcttcc tgatcggcct gctcgtcgtc gtcggcttct tcaacctgat
    11221 tttctgagcg cctttcaacg cggccagccg attgt
//
LOCUS       DATLGF010000369         4097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_61368,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000369 DATLGF010000000
VERSION     DATLGF010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61368"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VM406_11405"
     CDS             <1..688
                     /locus_tag="VM406_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVL76613.1"
                     /translation="YHDISHRSVDLIGIGPSGGKGRRDATVARMTIGFKDLGHGLIGR
                     LPYYEEGEYQPLYAQYPEVEKYYREVHEKRVATLGSTMRVSHSVGTIFPNMSFHGRQP
                     RTIAVFHPISATEMEMWRMYLVDKDAPEAVKDAARHYFMRYSGPGGMTESDDMENWTS
                     AAEACLGEQARSMFFNYQMGLGHARAVPELKGAVENGEFTEENARIFYGRWAQFMKGL
                     SWQQLMPGDE"
     gene            729..1268
                     /locus_tag="VM406_11410"
     CDS             729..1268
                     /locus_tag="VM406_11410"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: protein motif:HMM:NF007479.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HVL76614.1"
                     /translation="MADNTALQRLLLSAEISEFFSNEAELLDNRRYDEWLALLDERIA
                     YRMPLARNVRRDDAAREFTGENDIAWFDEGIATLRQRVAQIKTGVHWAEEPPSRVSHL
                     FTNIRLVDIQPSLDAPSEVTVSSRFLVYQNRHQAEVSLFVGKRVDVLTRHEGGWKVLK
                     RTIYLDQSVLQSKALTVFF"
     gene            1309..2463
                     /locus_tag="VM406_11415"
     CDS             1309..2463
                     /locus_tag="VM406_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVL76615.1"
                     /translation="MKSADAIIIGAGHGGVECAVALRAKGFAGSIALLSDEQQLPYER
                     PPLSKAMLAGTMDVERLRLRSEAIYAKNGIELALGRRIDRLDPAAGTATTTGGEVWAF
                     RHCVIATGARARRLAQPDGPGVYSIRSIDDVLAMRTALQPGMRLLVLGGGYLGLEAAC
                     TAAGLGARVHVIEQAPVIMSGRVSDVTAGRMHALHLEKGVSISTGAMVASWERTDTAW
                     RALTTDGAVHLADIVLVAIGATPNIEFAEAAGLACNGGILVDDACRSSAPNVFAIGDC
                     AARMNPELGRAIRIESVNNALVQARTVAAVLTGNTPDARKAPTFWSEQCGRRLQMAGL
                     ALPGTPCEDVARDTARGWLVERYQHGVLCAVEAVDSPVEFMQAAKRISIQ"
     gene            2480..2806
                     /locus_tag="VM406_11420"
     CDS             2480..2806
                     /locus_tag="VM406_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HVL76616.1"
                     /translation="MPQAVFVSPSGEEHVAEVPVGWSLMEAAREDGIEGVVAECGGGA
                     ICGTCHVKVEPKWFALLAQPQMMEAALLEAVPERSETSRLACQIMMSEELDGVRVHVP
                     SEQLEL"
     gene            2867..3868
                     /locus_tag="VM406_11425"
     CDS             2867..3868
                     /locus_tag="VM406_11425"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HVL76617.1"
                     /translation="MSTRPIVIVSATVPEDLKALITKDLDIHFVPVGESIEEKVPAEV
                     RAQAVGILCTVRSQIKESLLAGMPKLRVVSNFAVGYDNIDVPAATRANVLVCNTPGVL
                     DAAVADLTMGMVLCLARKLIAHDTFVRDGSWTKGAAPLTNDLAGKTLGLLGMGRIGRM
                     VAKRAKAFDMDVIYCNRRQDAEAEKAGLARYVDRDTLFKESDYVSVHIPLSAETKGGI
                     GEREFSMMKPSAYFLNTARGAVVDEEALIAALKSNKIAGAGLDVMVKEPMDPSNPLAS
                     LPNVVLQPHVGSATVETRRAMMALAVDNLVNAVSGTQPKAMVNADTWAAANQKAAHV"
     gene            3861..>4097
                     /locus_tag="VM406_11430"
     CDS             3861..>4097
                     /locus_tag="VM406_11430"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093600.1"
                     /note="catalyzes the formation of 3-methyl-2-oxobutanoate
                     from 2,3,-dihydroxy-3-methylbutanoate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HVL76618.1"
                     /translation="MSEKTYLGLDDGLTNYGDRDFSRYLRRSFVKAMGFTDATLDLPV
                     VGIAGNASDYNNCHRHFPELIEAVKRGVLVAGGLP"
BASE COUNT          760 a         1374 c         1301 g          662 t
ORIGIN      
        1 ctaccatgac atcagccacc gctccgtcga cctgatcggc atcggcccga gcggcggcaa
       61 gggccggcgc gacgcgaccg tcgcgcgcat gaccatcggc ttcaaagacc tgggccacgg
      121 gctgatcggc cgcctgccct actacgagga aggcgagtac cagccgctct atgcgcagta
      181 cccggaggtg gagaagtact accgcgaagt ccacgagaag cgcgtggcca cgctcggcag
      241 cacgatgcgc gtgtcgcaca gcgtcggcac gatcttcccg aacatgtcct tccacggccg
      301 gcagccgcgc acgatcgcgg tgttccatcc gatcagtgcg accgagatgg aaatgtggcg
      361 catgtacctg gtcgacaagg atgcgcccga agccgtgaag gacgcggcgc gccactactt
      421 catgcgttat tccgggccgg gcgggatgac cgaatccgac gacatggaga actggaccag
      481 tgccgccgag gcctgcctcg gcgagcaggc gcgcagcatg ttcttcaact accagatggg
      541 tctcggtcac gcgcgcgcgg tgccggaact caagggcgca gtcgaaaacg gcgaattcac
      601 tgaagagaac gcgcggatct tctacggacg ttgggcgcag ttcatgaagg gcttgagctg
      661 gcagcaactg atgccgggcg acgaatgagc ctcgtgtcca tcctccactg cgactgaaac
      721 ggaattccat ggccgacaac actgcattgc agcgcctgct cctgagcgcc gaaatcagcg
      781 agttcttcag caacgaagcc gaactgctcg acaaccggcg ctatgacgaa tggctcgccc
      841 tgctcgacga gcgcatcgcc taccgcatgc cgctggcgcg caacgtccgc cgtgacgacg
      901 ccgcgcgcga attcaccggc gagaacgaca tcgcctggtt cgatgaaggc atcgccacgc
      961 tgcgccagcg tgtcgcgcag atcaagaccg gcgtgcactg ggccgaggag ccgccttcgc
     1021 gcgtctcgca cctgttcacc aacatccgcc tggtcgacat ccagccttcg ctggacgcgc
     1081 cgagcgaggt gaccgtgtcc tcgcgcttcc tggtctacca gaaccggcat caggctgaag
     1141 tgagcctgtt cgtcggcaag cgcgtcgatg tgctgacccg ccatgagggt ggctggaagg
     1201 ttctgaagcg caccatctat ctcgatcaga gcgtgctgca gtcgaaggca ctgacggtgt
     1261 tcttctgaac gacgacgcac atcccctctt ccagaaactt aaataccgat gaaatcagcg
     1321 gacgcaatca tcattggcgc gggccacggc ggcgtcgaat gcgcggtggc cttgcgcgcc
     1381 aagggctttg ccggctcgat cgccctgctg agcgacgagc agcagcttcc ctacgagcgc
     1441 ccgccgcttt ccaaggcgat gctggccggc acgatggatg tcgaacgact gcgcctgcgt
     1501 tcggaggcga tctacgcgaa gaacggcatc gagcttgcgc tcgggcgccg catcgaccgc
     1561 ctcgatccgg ccgcaggcac ggccaccacg accggtggcg aagtctgggc tttccggcac
     1621 tgcgtgatcg ccaccggcgc gcgtgcgcgc cggctggcac aaccggacgg ccccggcgtg
     1681 tactcgattc gcagcatcga cgatgtgctg gccatgcgca ccgcactgca gccaggcatg
     1741 cgcctgctgg tgctgggcgg cggctatctc ggtctcgagg ccgcctgcac tgcagcgggc
     1801 ctcggcgcgc gcgtgcatgt gatcgagcag gctccggtga tcatgtccgg ccgcgtgtca
     1861 gacgtcaccg ccgggcgcat gcatgcgctg caccttgaaa agggcgtgtc gatctccacc
     1921 ggcgccatgg tggccagctg ggagcgcacc gataccgcat ggcgcgccct gactaccgat
     1981 ggcgcagtgc atctggccga catcgtcctc gtcgcgatcg gggcaacgcc gaacatcgag
     2041 tttgccgagg cggccggcct tgcctgcaac ggcggcatcc tggtcgacga tgcatgccgc
     2101 agctcggcgc ccaacgtatt cgcaatcggc gactgcgcgg cacgcatgaa tcccgagctc
     2161 ggacgcgcga tccgcatcga gagcgtgaac aacgcgctgg tgcaggcaag aaccgtggct
     2221 gccgtcctga ccggcaacac gcccgacgca cgcaaggccc ccaccttctg gtccgaacaa
     2281 tgcggtcgcc gcctgcagat ggccgggctg gcccttcccg gcactccctg cgaggacgtg
     2341 gcgcgcgata ccgcgcgcgg ctggctggtc gagcgctacc agcatggcgt gctgtgcgcg
     2401 gtcgaggcgg tcgacagtcc tgtggaattc atgcaggccg caaaacgtat ttccatccaa
     2461 tagccatccg gagaaaccaa tgccccaagc agtattcgta agccccagcg gtgaagaaca
     2521 tgtcgccgag gttcccgtcg gctggagcct gatggaagcc gcccgcgagg acggcatcga
     2581 aggcgtcgtc gccgaatgcg gcggcggtgc gatctgtggt acctgccatg tgaaggtcga
     2641 gcccaagtgg tttgccttgc tggcgcagcc gcagatgatg gaagccgcgc tgctcgaagc
     2701 cgtgcccgag cgtagcgaga ccagccgcct cgcctgccag atcatgatgt ccgaggaact
     2761 ggacggtgtc cgcgttcacg ttccctcgga acagctggaa ctctgatccg tcgcaggcat
     2821 ttgacgccct gccccctttt ttaaatctcg acacaaggaa aatcccatga gcactcgtcc
     2881 catcgtcatc gtctccgcca ccgttcccga ggacctgaag gccctgatca ccaaggacct
     2941 cgacattcat ttcgtcccgg tcggcgagag catcgaagag aaagtgcccg cggaagtgcg
     3001 cgcacaagcg gtcggcatcc tgtgcacggt gcgttcgcag atcaaggaat cgctgctggc
     3061 cggcatgccc aagctgcgcg tggtctccaa cttcgccgtc ggctacgaca acatcgacgt
     3121 tcctgccgcc acccgtgcga acgtcctggt gtgcaatacg ccgggcgtgc tcgatgcagc
     3181 ggttgccgac ctgaccatgg gcatggtgct gtgcctggcc cgcaagctga tcgctcacga
     3241 caccttcgtg cgcgacggct cctggaccaa gggcgccgca ccgctgacca acgatttggc
     3301 cggcaagacc ctgggcctgc tcggcatggg gcgcatcggc cgcatggtgg ccaagcgcgc
     3361 caaggccttc gacatggacg tgatctattg caaccgccgc caggacgcgg aagcggagaa
     3421 ggccggcctg gcacgctacg tcgaccgcga caccctgttc aaggagtccg actacgtcag
     3481 cgtgcacatt ccgctgtcgg ccgaaaccaa gggcggcatc ggcgagcgcg agttctccat
     3541 gatgaagccc tccgcctact tcctcaacac cgcgcgcggc gccgtggtag atgaagaggc
     3601 gctgatcgcc gcgctcaaga gcaacaagat cgccggcgcc ggcctggacg tgatggtcaa
     3661 ggagccgatg gacccgagca acccgctggc ttcgctgccg aacgtggtgc tgcagccgca
     3721 cgtgggcagc gcaacggtcg aaacccgccg cgcaatgatg gcgctggccg tggacaatct
     3781 cgtcaacgca gtctccggca cccagcccaa ggcgatggtg aacgccgaca cctgggccgc
     3841 cgccaaccag aaggctgcac atgtctgaaa agacctacct gggcctggat gacggcctga
     3901 cgaactacgg cgaccgcgat ttctcgcgct acctgcgccg ttccttcgtc aaggccatgg
     3961 gcttcacgga tgcgacgctg gatctgccgg tggtcggcat cgccggcaac gccagcgact
     4021 acaacaactg ccaccgccac tttcccgagc tgatcgaagc ggtcaagcgc ggcgtgctcg
     4081 tggccggcgg cctgccg
//
LOCUS       DATLGF010000370         6398 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_74413,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000370 DATLGF010000000
VERSION     DATLGF010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6398
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74413"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="VM406_11435"
     CDS             complement(<1..1152)
                     /locus_tag="VM406_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HVL76619.1"
                     /translation="MKMENPTPIQWQFSERAQQLQSSVIREILKVTVRPEVISFAGGL
                     PSPATFPVERMRAAFDTVLSNQGKVALQYGPTDGYGPLREWIAASLSTPEAKIVPEQV
                     LMVSGSQQGLDLLAKVLIDEGSKVLVETPSYLGALQAFSVYRPQFESVPTDDHGLIPE
                     RVADVAQGARLLYALPNFQNPTGRSLSVERRAALVETCARLGLPLIEDDPYGALSYRG
                     EPLPKMLSMNPNGVIYMGSFSKVLTPGIRLGYVVAPLPLVRKLEQAKQAADLHTAQLT
                     QMVVYEVIKDGFLAEHIPTIRKLYADQCQAMLDALQEYFPSSVTWTRPEGGMFIWVTL
                     PRHVDSMKLLDEAIAQNVAFVPGAPFYANAPESNTLRLSFVTVSPEKIRE"
     gene            complement(1351..1809)
                     /locus_tag="VM406_11440"
     CDS             complement(1351..1809)
                     /locus_tag="VM406_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HVL76620.1"
                     /translation="MSVYEKLKQLNIELPAVSAPAAAYVMHVQTGNTVFLSGHIAKKD
                     GKPWVGQLGKTMGTEEGKAAARAIAIDLMATLQDACGGDLNRVKRIVKLMSLVNSTPD
                     FTEQHLVTNGASELFGEVFGDKGKHARSAFGVAQIPLGACVEIELIAELA"
     gene            complement(1837..2736)
                     /locus_tag="VM406_11445"
     CDS             complement(1837..2736)
                     /locus_tag="VM406_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVL76621.1"
                     /translation="MKANPTNIDLSRETRGMALGLAGVAIFSLTLPFTRMAVAELNPV
                     LVALGRAVVAAAAAALLLWWLRAPRPTGAQWRALAITALGVVVGFPVFSSIAMRYVPS
                     AHGAIVIGILPLATAVVAALRFGERPSTGFWVAAVAGSAVVVAFAVVRGGGALHLADL
                     ALFAAVIAAAIGYAEGARLSQVMGGQQVISWALVLSLPVLLPATLWLGFEYGWEASPR
                     AWIGFGYVSLFSMFIGFFFWYKGLALGGIARVGQTQLLQPFLTLVGAALLLGEALEAA
                     HFLFAAAVIALVAVGRRMPVSRA"
     gene            complement(2744..3334)
                     /locus_tag="VM406_11450"
     CDS             complement(2744..3334)
                     /locus_tag="VM406_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HVL76622.1"
                     /translation="METLFPLALFAFVSSITPGPNNIMLTSSGILFGFSRSIPHMLGI
                     TFGFAVMLLLCAVGVGGLVLAVPGIHVALKSAGSAYLLYLAWNLRSMAFDGEARGSER
                     PMSFLGAALFQFANPKAWVMALTGVSAFLPVIQPAWLSIALFCLVFSAINLPCVGIWA
                     GTGAALRRYLSEPRWQRRFCAVMVGATTYSAVAIWL"
     gene            complement(3342..4328)
                     /locus_tag="VM406_11455"
     CDS             complement(3342..4328)
                     /locus_tag="VM406_11455"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine dehydratase"
                     /protein_id="HVL76623.1"
                     /translation="MNEVLPLASEIESAARAVYGAMRPTPQLAWPLLQAALGTEVWVK
                     HENHTPTGAFKIRGGLVYIDELKRREPDLAGVISATRGNHGQSVGFAARRHDLAATIV
                     VPRGNSTEKNAAMRALGVRLIEHGGEFQDAREHAAVLAREHGLHMIPSCHRDLVRGVA
                     TYWMEFFTACADLDVVFVPVGQGSGICAAIAAREALGMRTRIVGVVSAHALAYKLSYE
                     RGRITESPVGTVLADGMACRVPDAAAFDAIQRHVDDMVAVTDAEVARAMRLYFCATHN
                     VAEGAGAAALAAAMQQQPRLAGLRVGVTLSGGNVDQDSFARVLGEETESLVA"
     gene            complement(4325..4642)
                     /locus_tag="VM406_11460"
     CDS             complement(4325..4642)
                     /locus_tag="VM406_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HVL76624.1"
                     /translation="MVTCYLRYVLDPHKLEEFEHYGRLWMPLVEKFGGRHHGCFLPHE
                     GASSIAYALFSFPTLADYEQYRVRSQDDEACEAARRYAQETHCILSVERDFLRPMQRE
                     ARA"
     gene            4760..5596
                     /locus_tag="VM406_11465"
                     /pseudo
     CDS             4760..5596
                     /locus_tag="VM406_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233811.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            5784..6368
                     /locus_tag="VM406_11470"
     CDS             5784..6368
                     /locus_tag="VM406_11470"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HVL76625.1"
                     /translation="MDQAPFRKKTATRAAGLHAGGDLLRRAHGGATTSIMFNLPGVAS
                     HAVTCLDGHPMAKKGRAGSALLVAMIASFVAATIGIVIMMFFSPIIAETALKFGPAEY
                     LSMMVLGLLATTTLSTGSVIKGVVMVVLGVVLGTVSTDVNTGTVRFTFGIPYLYDGIS
                     LTALAMGLFGIADVLRSVNRLGKANVEGGAAGAA"
BASE COUNT         1151 a         2139 c         2049 g         1059 t
ORIGIN      
        1 ttcgcggatc ttctccggcg acaccgtcac gaaggacagg cgcagcgtgt tcgattccgg
       61 cgcgtttgcg tagaacggcg cgccgggcac gaaggccacg ttctgcgcga tcgcttcatc
      121 gagcagcttc atgctgtcga catgcctggg cagcgtcacc cagatgaaca tgccgccttc
      181 cgggcgcgtc caggtcaccg aggacgggaa gtactcctgc aacgcatcga gcatcgcctg
      241 gcactggtcg gcatacagct tgcggatggt cggaatgtgc tccgcgagga agccgtcctt
      301 gatgacctcg tacaccacca tctgcgtcaa ctgcgcggta tgcaggtcgg cagcctgctt
      361 ggcctgctcg agcttgcgca ccagcggcag cggcgcgacc acgtagccga gccggatgcc
      421 gggggtcagc accttcgaga acgaacccat gtagatcacg ccgttcgggt tcatcgacag
      481 catcttcggc agcggttcgc cgcggtagct gagcgcgccg tagggatcat cctcgatcag
      541 cggcaggccg aggcgggcgc aggtctcgac cagcgcggcg cggcgttcca ccgacaggct
      601 gcggccggtc ggattctgga agttcggcag cgcgtacagc aggcgcgcgc cttgtgcgac
      661 atccgccacg cgctccggga tcagcccgtg gtcgtcggtc ggcaccgatt cgaattgcgg
      721 acggtagacg gagaaagcct gcagcgcgcc gagatagctc ggtgtctcca ccagcacctt
      781 gctgccttcg tcgatcagga ccttggccag caggtccagc ccctgctgcg aacccgacac
      841 catcagcacc tgctccggga cgatcttcgc ctcgggagtc gaaagggatg ccgcgatcca
      901 ttcgcgcagc gggccatagc cgtcggtggg tccgtactgc aacgcgacct tgccctggtt
      961 cgacagcacg gtgtcgaagg ccgcgcgcat gcgctctacc gggaaggtgg ccggggacgg
     1021 caatccgccc gcgaacgaga tcacttccgg acgcacggtg actttcagga tttcgcggat
     1081 gaccgagctt tgcagctgct gcgcacgctc ggagaattgc cattggatcg gggtcgggtt
     1141 ttccattttc atgcttgtct cacttgttgt ttgcggcctt gccgcgtaat cggctcgctt
     1201 gtggcttgcc ctcgatggag cgacgccggg taggcgccgc ccgctggatt cgtcaaatcg
     1261 tcgattatcg ctgttcgcta gtgggatggc agaagggtcc tcccggcttc agcggatgag
     1321 gtccctcgct gcgctcggga cgacagggat tcaggccagc tcggcgatca gttcgatttc
     1381 cacacaggcg cccagcggaa tctgcgcgac gccgaatgcg gaacgcgcat gcttgccctt
     1441 gtcgccgaac acttcgccga acagttcgga ggcgccgttg gtgaccaggt gttgctcggt
     1501 gaaatccggc gtcgagttca ccaggctcat cagcttgacg atgcgcttga cgcggttcag
     1561 gtcgccgccg caggcatcct gcagcgtggc catcaggtcg atcgcgattg cgcgcgcggc
     1621 cgccttgccc tcttccgtcc ccatggtctt gccgagctgg ccgacccagg gcttgccatc
     1681 ctttttcgcg atatggccgg acaggaagac cgtattgccg gtttgcacat gcatcacgta
     1741 ggcggcggcg ggcgcggaga cggcggggag ttcgatgttc agctgtttga gtttttcgta
     1801 gacggacatg atggggctcc ggaaaggttg gcatgatcac gcgcgtgaca ccggcatccg
     1861 gcgtccgacg gcgactaggg cgatcactgc ggcggcgaac aggaagtgtg cggcttcgag
     1921 cgcttctcca agcagcaagg cggcgccgac cagcgtcagg aacggttgca gcaactgcgt
     1981 ttgtccgacg cgggcgatgc cgcctagagc gagaccttta taccagaaga agaacccaat
     2041 aaacatcgaa aaaagggaga cgtagccgaa tcctatccag gcgcgcggcg atgcttccca
     2101 accatattcg aaaccgagcc agagcgtggc cggcagcagc accggcagcg acagcaccag
     2161 tgcccacgag atcacctgtt gcccgcccat cacctgcgac aggcgtgcgc cttcggcata
     2221 gccgatcgcc gcggcgatca cggcggcaaa cagggcgaga tcggccaggt gcagtgctcc
     2281 gccgccgcgc accacggcga acgccaccac cacggcgctg cctgcaacgg cagccaccca
     2341 gaacccggtc gagggccgct cgccgaagcg cagtgcggcg acgaccgcgg tggccagcgg
     2401 caggatgccg atcacgatcg cgccgtgcgc cgacggcaca tagcgcatcg caatcgagct
     2461 gaataccgga aagccgacca cgacgccaag ggcggtgatt gccagcgcgc gccattgcgc
     2521 cccggtcgga cggggggcgc gcagccacca cagcagcagc gcggccgctg ccgctgcaac
     2581 gacggccctg ccgagcgcaa ccagcaccgg attcagctcc gccaccgcca tgcgcgtaaa
     2641 cggcagcgtc aggctgaaga tcgccacgcc ggccagaccc agcgccatgc cgcgggtttc
     2701 ccgcgacaga tcgatattcg tcgggttcgc cttcatatcg ccttcatagc cagatcgcca
     2761 cggccgaata ggtggtcgcg ccgaccatca ccgcgcagaa ccggcgctgc cagcgcggct
     2821 cggacaggta gcggcgcagc gccgcgcccg tgccggccca gatgccgacg caaggcaggt
     2881 tgatcgccga gaacaccagg cagaacagcg cgatcgacag ccaggccggc tgaatcaccg
     2941 gcaggaaagc cgacacgccg gtcaatgcca tcacccatgc cttcggattg gcgaactgga
     3001 acagcgcggc gccgaggaag gacatcgggc gctcgctgcc cctcgcctcg ccgtcgaagg
     3061 ccatgctgcg caaattccat gcgagataca gcagataggc cgaaccggcc gacttcagcg
     3121 cgacatggat gccgggcacc gcgaggacca acccgccgac gccgaccgcg cacagcaaca
     3181 gcattaccgc gaagccgaag gtgatgccga gcatgtgcgg aatcgaacgc gaaaagccga
     3241 acaggatgcc ggacgaggtg agcatgatgt tgttcggtcc cggcgtaatc gaggatacga
     3301 aggcgaacaa tgcgagtggg aacagggttt ccatggtttc tctatgcgac aagcgactcg
     3361 gtttcctcgc ccagcacccg ggcaaaacta tcctggtcga cgttgccgcc ggagagcgtg
     3421 acgccgacgc gcagcccggc cagccgcggc tgctgctgca tcgcggccgc gagcgcggcc
     3481 gcacccgctc cttcggcgac gttgtgcgtc gcgcagaagt aaagacgcat cgcacgcgcc
     3541 acctcggcgt ccgtcacggc gaccatgtcg tcgacgtgcc gctgaatggc gtcgaatgcg
     3601 gcggcatcgg gcacgcggca ggccatgccg tcggcaagca cggtgccgac cggcgactcg
     3661 gtgatccgcc cccgctcgta cgaaagcttg taggcaagcg catgggcgga caccacgccg
     3721 acgatgcgtg tcctcatgcc gagcgcctcg cgcgcggcga tcgccgcgca gatgcccgag
     3781 ccctggccga ccggcacgaa aaccacatcc agatcggcac aggcggtgaa gaactccatc
     3841 cagtaggtgg ccaccccgcg caccaggtcg cgatggcagg acgggatcat gtgcaagccg
     3901 tgctcgcgtg ccagcacggc ggcatgctcg cgcgcatcct ggaattcgcc accatgctcg
     3961 atcagccgca cgccgagcgc gcgcatcgcc gcattcttct cggtgctgtt cccgcgcggg
     4021 accacgatgg tggccgcaag atcatggcgg cgcgccgcga acccgaccga ctggccgtgg
     4081 ttgccgcgtg tggccgagat cacacccgcc agatccggct cgcgccgctt cagctcatcg
     4141 atatagacca ggccgccgcg gatcttgaac gcgccggtcg gcgtgtgatt ttcgtgcttg
     4201 acccacacct cggtgccgag cgcggcctgc agcagcggcc aggccagttg cggtgtcgga
     4261 cgcatcgcgc catacacggc ccgtgcggcg gactcgatct cgctcgccag cggcagcacc
     4321 tcgttcatgc gcgcgcctcc cgctgcatcg ggcgcaggaa atcgcgctcg acgctcagga
     4381 tgcagtgcgt ctcctgagcg taacggcgcg ccgcctcgca cgcctcgtcg tcctgcgagc
     4441 ggacccggta ctgctcgtaa tcggccagcg tcggaaacga gaacaatgcg taggcgatgc
     4501 tgcttgcgcc ctcgtgcggc aggaaacagc cgtgatgccg tccgccgaac ttctccacca
     4561 gcggcatcca cagccgtccg tagtgttcga actcctccag cttgtggggg tcgagcacgt
     4621 aacgcaggta acaggtcacc acagcgcctc ccggtcttcc gcatgttgcg gcaggttgat
     4681 cgatgaccta tgctaaacga caaaaccagt acactaccgg tacagtcaat caattagttt
     4741 cgatcactgt catggcgata tgaccgatac agcccgacac ccaacccctt ccggccgcgg
     4801 ccgcggcagc accaccctga cactggttgc gcccgccctg ctctcgcgcg gcggcggcga
     4861 atcgctggtg gagcagatcg tgcgctcgat ctgctcgcgc atcgacgaca agctgttgcg
     4921 cagcggcgcg cgcatgccgt cgatccgcca gttcgccgac gcgcacggcg tttcgcgctt
     4981 caccgtggtg gaggcctatg accggctggt ggcccagggc tacctcgagt cccgccgcgg
     5041 ttcaggcttc tacgtgcgcg aacgggcacc gatggcgccg ccgggcacgc aggcgcgcag
     5101 cgacgaggtg ccgaccggac agatggacgt ggtctggctg gtgcgcaaca tgttccgcga
     5161 gcatccgcca cagcgcatgc cgggctcggg cctgctgccc gcggactggc tggacgggga
     5221 actgctcgcg cacggcctgc gcgcggtcag ccgccagaac accaacctgc tgctccagta
     5281 cggcaatccg cagggttttt tgccgctgcg ccagcagctg caattgaagc tggccgaact
     5341 cgagatcgcc gcagcgccgg aacagatcct caccacctcc ggcatcacgc agggcctgga
     5401 catcgtcgcg cggcatttcg cccagcccgg cgacaccatc ttcgtcgacg atccggcctg
     5461 gttcctgatg ttcggctcct tcgccacgct gggcttcaag gtgatcggca ttccgcgcct
     5521 ggccgacggg cccgacatcg cccggctggt cgagctggcc gcgatccatc ggcccaagct
     5581 gtacgtgatc aattccttcg catctactga cgttatatgt gcattccatt acgcgccctc
     5641 tacacacgta gcacgtcagc gcgttatacc ggggaccctg cggtattcct cgtaagccgc
     5701 tcgtagcaga acgcttataa caagaaaaag tggaagggcc ttccaaagat cgggtctagc
     5761 cgctctgcct cataagcctg acaatcgacc aggcaccctt tcgcaaaaaa acagccacgc
     5821 gtgccgcggg gctccatgct ggtggggatc tactacggcg cgcacacggc ggcgcgacca
     5881 cctcgatcat gttcaacctg ccaggcgtgg cctcgcatgc agtgacctgt ctggacgggc
     5941 acccgatggc gaagaaaggc cgcgccggct cggcattgct ggtggcgatg attgcctcgt
     6001 tcgtggctgc aaccatcgga atcgtgatca tgatgttctt ctcgccgatc atcgccgaga
     6061 ccgcgctgaa gttcggcccg gcggaatact tatcgatgat ggtgctgggc ctgctggcca
     6121 ccaccacgtt gtctaccggt tccgtgatca agggtgtggt gatggtggtg ttgggtgtgg
     6181 tgctgggtac tgtcagcacc gacgtcaaca ccggcaccgt gcgctttacc tttggcatcc
     6241 cctacctgta tgacggcatc agcctgaccg cgctggcgat gggcctattc ggtatcgccg
     6301 atgtgctgcg cagtgtgaac cgtctgggca aggccaacgt ggagggcggg gcggctggcg
     6361 cagcctgaag ctgacacgcg aggaagcccg ccgctcgg
//
LOCUS       DATLGF010000371         5360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_103362, whole genome shotgun sequence.
ACCESSION   DATLGF010000371 DATLGF010000000
VERSION     DATLGF010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103362"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..4608)
                     /locus_tag="VM406_11475"
     CDS             complement(148..4608)
                     /locus_tag="VM406_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVL76626.1"
                     /translation="MLRNFFTRTGFRRQLTVTVGAAIFGLALFSALMNSREASDRMEA
                     HLVEQGIHIAENLARQSALGLLYRSAENVADGVELTLAFPDVQHVEIIDADGHVLVSR
                     WRPEAESPGVKSDAPLVPAPHAMLVRETRNAWHFSAPAYGGRKQSTPFDLEEAEQQRL
                     GQVNVAISKDSLHRLGTSLLIGNLVISLSFAAILLALMRILAGRILQPLTSLRQLMGR
                     AESGESGMRAAPAGPRDIVEMAHAFNTMMDVLEEREAELKRSRDDAVRTALMKTQFAA
                     TVSHEVRTPLNGVVGMLDMLKEMPLSKRQQECVDVAWNASRALIELTDDILDFSRMEA
                     GKLELEHVEFDLRRAVEDVIELLARQAQQKGLEIGYVRDPAVPLRVTGDSLRLRQVLL
                     NLLGNAVKFTERGEVALRLSCAADGDALLLRFEVVDTGIGINGDALRHIFESFAQADR
                     STTRRYGGAGLGLAICRQLVELMGGTIGVSSEVGKGSTFWFTVRCGHVSGTAALLSAP
                     AEFQDLRVLVADSSRIVRQFVDTTLTGGGIDCATCESGMEAVAELWRAQSCGTPYAVV
                     VADAAAVDAHGVALVERIRSERDLSPPQLLLLDRYATPIGATASGADLCLGKPLREER
                     LWEAMRRLLLRTDRPAAMFAAPSPGDAPQPSRDYRVLVAEDNRTNQMVAAGMLAMNGC
                     ACEFAANGREALELARRTRFDLILMDCSMPEMDGYEATGHIRAFEQALGLHTPIIAMT
                     ANTQPGDAEKCLAAGMDDYLAKPITLAELRARLERWLGGGDIRAQVPLPHIGKDGRDS
                     GALDHEAFDKLRDVLGPTLQQSVSFFLEDTPAYLNQLEQAVEEGNADHARAMAHAIKG
                     SSGNFGAAGLTALAREAEELAIERRPQEIRALLPRLRQAFDAVAAALGAEVLIEDRLA
                     VREGRSAAQVLIVDDDRSTRTALRNTLQRDGFRVDEASNGAQALLMLKRMQPDVILMD
                     AVMPVMDGFTACARVQELPKGNTIPVLMITALDDESSIERAFAAGASDYIPKPIHFGV
                     LSQRVRRIIEANQAEKRIRHMAYNDMLTGLPNRAMFIDQLGRHIEQARVAGESIAVLF
                     LDLDRFKNVNDSLGHDVGDRLLVAAAQRIRRSVRNADCVARLGGDEFTVVLTGISGAN
                     AAVSAAQNICRALAAPFQIDGHDMFITASVGISLYPHDGTDVGTLLKHADTAMYRAKR
                     TNSGFQFFEPSMELSISHHVRMESDLRQALDRNELELYFQPQAHAKSGRVVGAEALLR
                     WRHPGRGMVSPSDFIPLAEETGLIIPIGSWVLRRACQQMRAWLNDGMAPIRVAVNLSV
                     KQLLQKNFAAEVERVLADTQLPAELLELEITESTLMEHAQDTMRALQRLRSIGVRLSI
                     DDFGTGYSSLAYLKRFDVDIIKIDRSFVRDVPQDADDAAIAAGIIVLAHSLRLEVVAE
                     GVETEAQLEFLKERNCDLVQGYMLSPPVPAEQFRRLVIDSDLPVLAGGRK"
     gene            complement(4699..>5360)
                     /locus_tag="VM406_11480"
     CDS             complement(4699..>5360)
                     /locus_tag="VM406_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVL76627.1"
                     /translation="ADVPVIVGGVLAGPEILPIRETETRGSTGISLTPDPALLFARLK
                     FFFPHTRRVLVVYSAQRNEWLIRLAREAARSHGLELIALEASDLGSAVRHYAHAFAAA
                     DGRRDAVWLLQDAATLDENTILPLVLRESWNRNVPVFSSSFQHVKKGVLFALYPDNIQ
                     LGRSLAASAEQALSGDARRGVLLPLRDVHLAVNLRTAGHLGLRFSRQQQRSFDMVFPE
                     P"
BASE COUNT          886 a         1793 c         1772 g          909 t
ORIGIN      
        1 gcgtgtggac ttcggtggcg tgatggtgac ctacggcccg aaggatcact ccggcagcga
       61 gttcgtcgag ctgaccatga tcggcaggaa cggacgcatc atccgctagt caatgcgggc
      121 tggccgccgg cgggcgccgc tgcccgccta cttcctgcca ccggccagaa cgggcaggtc
      181 gctatcgatc accagccgcc ggaactgctc ggcaggaacc ggcggactga gcatgtaacc
      241 ctgcaccagg tcgcagttgc gctccttcag gaactccagc tgggcctcgg tctccacccc
      301 ttccgccacc acctccagcc gcaggctgtg cgcgagcacg atgatgcctg ccgcgatcgc
      361 ggcgtcgtcg gcatcctgcg gcacgtcacg cacgaacgag cggtcgatct tgatgatgtc
      421 cacatcgaag cgcttcaggt aggcgagcga tgagtagccg gtaccgaagt cgtcgatcga
      481 cagccgcacg ccgatgctgc gcagccgctg cagcgcacgc atcgtgtcct gcgcgtgttc
      541 catcagcgtg ctctcggtaa tctccagttc gagcagttcc gccggcagct gggtatcggc
      601 gagcacgcgc tcgacctccg ccgcgaagtt cttctgcagc agctgcttga ccgacaggtt
      661 caccgccacc cggatcggcg ccatgccgtc gtttagccag gcgcgcatct gctggcaggc
      721 gcggcgcaag acccaggagc cgatcggaat gatcaggccg gtctcttcgg ccaacgggat
      781 gaagtcactg ggcgagacca tgccgcgccc cgggtgacgc cagcgcagca aggcctccgc
      841 accgaccacg cgcccgctct tcgcatgtgc ctgcggctgg aaatacaact ccagctcgtt
      901 gcggtcgagt gcctggcgca gatcgctttc catgcgtacg tgatgggaaa tggacagctc
      961 catcgacggc tcgaagaact ggaagccgct gttggtgcgc ttggcgcggt acatcgcggt
     1021 atcggcatgc ttgagcagcg tgccgacatc ggtgccgtcg tgcgggtaca gcgagatgcc
     1081 gacgctggcg gtgatgaaca tgtcatggcc gtcgatctgg aacggcgcgg ccagcgcgcg
     1141 gcagatgttc tgcgcggccg aaaccgccgc gttggcgccg gagatcccgg tcagcacgac
     1201 ggtgaattcg tcgccgccga gacgggcgac gcagtcggca ttgcgcacgc tgcggcggat
     1261 gcgctgggcc gcggccacca gcaggcgatc gccgacatcg tggcctaggc tgtcgttgac
     1321 gttcttgaag cggtcaagat cgaggaacag caccgcgatc gactcgccgg ccacgcgcgc
     1381 ctgctcgatg tggcggccga gctgatcgat gaacatcgcg cggttcggca agccggtcag
     1441 catgtcgttg taggccatgt ggcggatacg cttttccgcc tggttggctt cgatgatgcg
     1501 gcgcacccgc tgcgacagca ctccgaagtg aatcggtttg ggaatgtagt cgctcgcgcc
     1561 ggcggcgaac gcgcgttcga tcgaactttc gtcatcgagc gcggtgatca tcagcaccgg
     1621 gatggtgttg cccttcggga gctcctgcac gcgggcgcag gcggtgaacc cgtccatcac
     1681 cggcatcacg gcatccatca ggatcacgtc cggctgcatg cgcttgagca tcagcagcgc
     1741 ctgcgcgccg ttcgaggctt cgtcgacgcg gaagccgtca cgttgcagcg tgttgcgcag
     1801 cgcggtgcgg gtgctgcggt cgtcgtcgac gatcagcacc tgggccgcgc tgcggccttc
     1861 ccgcaccgcg agccggtcct cgatcagcac ttccgcgccc agcgccgccg ccaccgcatc
     1921 gaaagcctgg cgcaggcgcg gcagcaaggc gcggatctcc tgaggccgcc gctcgatcgc
     1981 cagctcttcg gcctcgcgcg caagcgcggt cagtccggca gcgccgaaat tgccgctgct
     2041 gcccttgatc gcgtgcgcca tcgcccgtgc gtggtcggca ttgccttcct cgaccgcctg
     2101 ttcgagctgg ttcaggtaag ccggcgtgtc ctccaggaag aagctcaccg actgctgcaa
     2161 tgtcggcccg agcacgtcgc gcaacttgtc gaacgcctcg tggtcgagtg ccccgctgtc
     2221 gcgcccgtcc ttgccgatat gcggcagcgg cacctgcgcg cggatgtccc cgccgcctaa
     2281 ccagcgctcc agacgtgcac gtagctcggc cagcgtgatc ggcttggcga ggtagtcgtc
     2341 catcccggcc gccaggcatt tctcggcgtc gcccggctgg gtattggcgg tcatcgcgat
     2401 gatcggcgtg tgcagcccga gcgcctgctc gaacgcacgg atgtgcccgg tcgcctcgta
     2461 gccgtccatc tccggcatgc tgcaatccat caggatcagg tcaaagcggg tgcggcgtgc
     2521 cagttccagc gcctcgcggc cattggccgc gaattcgcac gcgcagccgt tcattgccag
     2581 catgccggcg gccaccatct ggttggtgcg attgtcttca gcgaccagta cccggtagtc
     2641 gcgcgatggc tgcggcgcat cgcccggcga gggggcggcg aacatggcgg cgggacggtc
     2701 ggtgcgcagc aacagccggc gcatcgcctc ccaaaggcgt tcctcgcgca gcggcttgcc
     2761 caggcagaga tcggcgccgc tggcggtcgc gccgatcggc gtcgcgtaac ggtcgagcag
     2821 cagcaattgc ggcggggaca ggtcgcgctc gctgcggatg cgttccacca gcgccacgcc
     2881 atgcgcgtcg acggcggccg cgtcggcaac gacgaccgca tacggcgtgc cgcaggactg
     2941 cgcgcgccac agctcggcca ccgcttccat gccgctctcg caggtcgcgc agtcgatgcc
     3001 tccaccggtc agcgtggtgt cgacgaactg gcgcacgatg cggctggaat ccgccaccag
     3061 cacccgcagg tcctggaatt ccgccggtgc gctcaagagc gccgcggtgc ccgacacgtg
     3121 gccgcatcgc acggtgaacc agaatgtgct gcccttgccg acttcgctgg acacgccgat
     3181 cgtgccaccc atcaattcga ccagctgcct gcagatcgcc agccccaggc cggcgccgcc
     3241 atagcggcgc gtggtcgagc gatcggcctg tgcgaacgat tcgaagatgt ggcgcagcgc
     3301 gtcgccgttg atgccgatgc cggtatcgac aacctcgaaa cgcagcagca gcgcgtcacc
     3361 gtcggccgcg caggacaggc gcaatgcgac ctcgcctcgc tccgtgaact tcaccgcatt
     3421 gccgagcagg ttcagcagca cctggcgcag acgcagcgaa tcgccggtca cgcgcagcgg
     3481 caccgcgggg tcgcgcacgt agccgatctc cagccccttc tgctgggcct ggcgcgcaag
     3541 cagctcgatc acgtcctcga ctgcgcggcg caaatcgaat tcgacatgct ccaattccag
     3601 cttgccggct tccatgcgcg agaaatcgag gatgtcgtcg gtcagctcga tcagcgcgcg
     3661 cgaggcgttc cacgccacgt ccacgcactc ctgctggcgc ttgctcagcg gcatttcctt
     3721 gagcatgtcc agcatgccga ccacaccgtt cagcggcgtg cgcacctcat ggcttacggt
     3781 cgcggcgaac tgcgtcttca tcagcgcggt gcgcaccgcg tcgtcccgcg agcgcttcaa
     3841 ttcggcttcg cgctcttcca gcacatccat catggtgttg aatgcatgtg ccatttcgac
     3901 gatatcgcgg ggaccggccg gggcggcgcg catgcccgac tcgccggatt ccgcgcggcc
     3961 catcaactgc cgcagcgaag tcagcggttg caggatgcgg ccggccagga tacgcatgag
     4021 cgcaagcagg atcgccgcaa acgacagcga gatgacgagg ttgccgatca acagcgaggt
     4081 gccgagacgg tgcagcgagt ccttgctgat ggcgacattg acctggccaa ggcgctgctg
     4141 ctcggcttcc tcgaggtcga aaggcgtgct ctgcttgcgg ccgccatagg ccggggcgct
     4201 gaagtgccat gcgttacgtg tttcccttac gagcatggcg tgcggcgccg ggacgagcgg
     4261 ggcatcgctc ttgacgccgg gcgactcggc ttccggacgc caccgggaaa ccagcacgtg
     4321 accatcggcg tcgatgatct cgacgtgctg tacatccggg aatgcgaggg tcagttcgac
     4381 cccatccgcg acattctcgg cggaccggta cagcaagccc aaggcacttt gccgcgccag
     4441 gttttcggcg atgtgtatgc cctgctccac caggtgcgct tccatccggt cgctcgcctc
     4501 cctggagttc atcagggcgg aaaatagcgc aaggccgaaa atcgcggcgc ctacggtaac
     4561 cgtcagctgg cgccggaatc cggtacgggt gaaaaaattg cgcagcatgg cttctcctca
     4621 gcgggctctg cgcacagaac gcgggttgaa agcaggttgc gggtgatgat ggggcatgtg
     4681 aagcgttcag atcaatgttc agggttcggg aaagaccatg tcgaagctgc gctgctgctg
     4741 ccggctgaac ctgagcccaa ggtggccagc ggttctcagg ttgacggcga gatgcacgtc
     4801 gcgcaacgga agcagcacgc cacgccgagc gtcgccgctc aaggcctgct cggccgaggc
     4861 agcgagcgag cggccaagct ggatgttgtc cgggtacagg gcgaacagca cgcctttctt
     4921 tacgtgctgg aagctgctgg agaatacggg aacgttgcga ttccaggatt cgcgcagcac
     4981 caggggcagg atcgtgttct cgtccagggt ggcggcatcc tgcagcagcc agactgcatc
     5041 ccgtcggccg tccgcagcgg caaatgcgtg tgcgtagtgg cgcaccgcgc tgcccagatc
     5101 actggcttcg agcgcaatca attccagccc gtggctgcgt gcggcttcac gcgcgaggcg
     5161 gatcaaccac tcgtttcgct gcgcgctgta gacgaccagg accctgcgcg tgtgcggaaa
     5221 gaagaacttc agccgcgcga acaggagtgc gggatcgggc gtgaggctga tgccggtgct
     5281 gccccgcgtt tccgtctcgc ggatcggcag gatttccgga ccggccagca cgccgccgac
     5341 gatcaccggc acatccgcgt
//
LOCUS       DATLGF010000372         4305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_182650, whole genome shotgun sequence.
ACCESSION   DATLGF010000372 DATLGF010000000
VERSION     DATLGF010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182650"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="VM406_11485"
     CDS             complement(<1..1177)
                     /locus_tag="VM406_11485"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HVL76628.1"
                     /translation="MGEQDEQLHRFLEQAFQERIGRFPQTLTALGRRDQKDRLDDQSE
                     SAAQDFRALLVGQLEAIGAFARDKLSPHAQLNVRVFEQDARQTLARLQYRAQEYLVNQ
                     KFGLHTDFPAFMINMHGVADLQDARDYVARLRAFGRACDQVIEELERREAMGIVPPAF
                     LFPQMLDDCRRFVGDPSDTQPVDANVLLADLRAKLAELGDRGDPGEAGKDLLQAAAQA
                     LRETVLPGYERLIAFLERQESRAPAEGGAWSLPDGEAYYRMCLARHTTTALSADEIHR
                     LGLDEVARIRGEMIALKGALGFGGYGDDELGTLFSHARDHDGFYYPQTPAGRAAYMEE
                     MNAAIVEMKTLLPQYFASLPKDELLVKAVEPHRERTAGIAFYQGPAEDGSRPGIFYIN
                     "
     gene            complement(1182..1841)
                     /locus_tag="VM406_11490"
     CDS             complement(1182..1841)
                     /locus_tag="VM406_11490"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HVL76629.1"
                     /translation="MTFSIVARCPRTGQIGVAAATALQAVGKLACHALPKVGAVASQA
                     LLNPYLAYDGLRLMEQGVPARTALERVLAGDPDANSRQVGIVDRDGNVAAWTGGENIE
                     WAGDRQEANFSTQGNRLAGPQVLERVVETMLDTEELDLSERLCLALQAGIEVGGDTKG
                     ERSVNILVYGEEEYPLCDIRIDDHDDPLNELRRLFKLYQDEILPNMQKMPRRADIPRP
                     L"
     gene            1957..2033
                     /locus_tag="VM406_11495"
     tRNA            1957..2033
                     /locus_tag="VM406_11495"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1991..1993,aa:Met,seq:cat)
     gene            complement(2073..3395)
                     /locus_tag="VM406_11500"
     CDS             complement(2073..3395)
                     /locus_tag="VM406_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76630.1"
                     /translation="MNDATILPFERSVGCGMHASALNNGCFCIGLDRSAVRHAFAAQQ
                     GVPGLAELVDERCPHLFSAHPVFVEAGHIARMREIVAAVEHVVALPAWQEQVLARSPV
                     AARHETPARSVFFGYDFHVGLDRIGLIEINTNAGGALLNAVLGAAQRGCCAPVEALFP
                     VVEAEAAEQRMLDMFRNEWRLAGRAGALQSVAIVDHDPQNQYLYPEFLLFRELFRRNG
                     ITAVVADPARLRFDSGRLCYDDQPIDLVYNRSTDFAFENPASAALRAAWFADAAVITP
                     HPRAHALYADKANLALLSDPQALEHLGVARDVREVLAAGIPRTERVTAEQAESLWARR
                     RDLFFKPRAGFGSKAAYRGDKITRRVWKEICEGDYVAQAIVAPGQRLPARHAEAMKFD
                     LRAYVYDGAVQQLAARLYQGQTTNFRTPGGGFAPVYAVPEAASSACAA"
     gene            complement(3397..>4305)
                     /gene="arsB"
                     /locus_tag="VM406_11505"
     CDS             complement(3397..>4305)
                     /gene="arsB"
                     /locus_tag="VM406_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HVL76631.1"
                     /translation="AHVNIPVGILIWAMIVPMLMKIDFSSLHEVRRQEAGVGITLFVN
                     WAVKPFSMALFGWFFLRHVFAPWLPAAQIDSYIAGLILLGAAPCTAMVFVWSNLCRGD
                     ANFTITQVALNDVVMLFAFAPIVALLLGVSSIPVPWDTLLLSVVMYIAIPLAIAQFLR
                     GRLRARGSGVFEAALARVTPFSIVALLATLVLLFAFQGRQIIEQPLIIAILAVPILLQ
                     VLFIAALGYWLNRKMRVAHSVAGPSTMIGASNFFELAVAVAIVLYGFDSGAALATVVG
                     VLIEVPVMLALVRMVNRTRTWYEAAH"
BASE COUNT          753 a         1416 c         1416 g          720 t
ORIGIN      
        1 ggttgatata gaagatgccg ggacggctgc cgtcttccgc cggtccctgg tagaacgcaa
       61 tgccggccgt gcgctcgcgg tggggctcga ccgcttttac cagcagttcg tccttcggca
      121 aggacgcgaa gtattgcggc agcagcgtct tcatttcgac gatcgccgcg ttcatctcct
      181 ccatgtaggc cgcgcgcccg gccggcgtct gcggatagta gaagccgtcg tggtcgcgcg
      241 catgcgagaa cagcgtgccg agctcgtcat ccccgtaccc gccgaagccg agcgcaccct
      301 tcagcgcaat catttccccg cgaatgcgcg cgacctcgtc cagccccagc cggtgtatct
      361 cgtcggcgga gagcgcagtg gtcgtgtgcc gcgccaggca catccggtag taggcttcgc
      421 cgtccggcag cgaccaggcg ccgccttcgg cgggtgcccg gctttcctgc cgctcgagaa
      481 acgcgatcag gcgttcgtaa cccgggagga ccgtctcgcg cagcgcctgc gcggctgcct
      541 gcagcaggtc tttcccggcc tcgccgggat cgccgcgatc gccgagctcg gcgagcttgg
      601 cacgcaagtc ggcaagcaag acgttggcat cgacgggctg cgtgtcgctc gggtcgccga
      661 cgaagcgccg gcagtcgtcg agcatttgcg ggaacaggaa ggcgggcggc acgatgccca
      721 tcgcctcgcg ccgctcgagc tcttcgatga cctggtcaca ggcgcgcccg aacgcgcgca
      781 ggcgtgcgac atagtcgcgt gcgtcctgca ggtcggcgac cccgtgcata ttgatcatga
      841 acgccgggaa gtcggtatgc aggccgaact tctggttgac caggtactcc tgggcgcggt
      901 attgcagacg agcgagcgtc tggcgcgcat cctgttcgaa cacgcgcacg ttcaactgtg
      961 cgtgcggcga cagcttgtcg cgcgcgaacg cgccgatcgc ttcgagctgg ccgaccagca
     1021 gcgcgcggaa atcctgcgcc gcggattccg attgatcgtc cagccggtct ttctgatcgc
     1081 gacggccgag cgcggtgagc gtttgcggaa agcggccgat ccgttcctgg aacgcctgct
     1141 cgaggaaacg atggagctgt tcgtcctgct cacccatcgc gttacagcgg ccgcggaata
     1201 tcggcccgtc gcggcatctt ctgcatgttc ggcaggatct cgtcctgata cagcttgaaa
     1261 agccggcgca attcgttcaa cgggtcgtcg tggtcatcga tgcggatgtc gcagagcggg
     1321 tattcctctt cgccgtacac gaggatgttc accgatcgtt cgcccttggt gtcgccgccg
     1381 acttcgatgc cggcctgcag cgcgaggcaa agccgttcgg agagatccag ctcttccgtg
     1441 tccagcatgg tctcgacgac gcgctcaagc acctgcggac cggcgagccg gttgccctgg
     1501 gtactgaagt tcgcttcctg ccggtcgcca gcccattcaa tattctcgcc tccggtccag
     1561 gcggcgacat tgccgtcccg gtccacgatg ccgacctgac ggctgttcgc atccggatca
     1621 ccggccagca cgcgttcgag cgccgtgcgg gccggcacac cttgctccat cagccgaagg
     1681 ccgtcgtatg cgagataggg attgagcagc gcctgggacg ccactgcgcc gactttcggc
     1741 agcgcgtggc aggcgagctt gcccacggcc tgaagggcgg tggccgcagc gacgccgatc
     1801 tgtccggtcc ttggacagcg tgcaacgatg gaaaaagtca tggtcacctc ctgccagttc
     1861 gacaagagtc ggccgggtgc gttcaggctt ttcgcgcgcc aacagattga gcgcttgcgc
     1921 ccggggcgga tgctctgtag aattcgcccc ttcagacgcg gggtggagca gtctggcagc
     1981 tcgtcgggct cataacccga aggtcacagg ttcaaatcct gtccccgcaa ccagacacag
     2041 aaaaaaccgg ccttgcgccg gttttttcgt tttcaggccg cgcaggcaga acttgccgcc
     2101 tctggcaccg catagacggg cgcgaaaccg ccgcccgggg tgcggaagtt ggtggtttgc
     2161 ccctggtaca gccgcgcggc cagttgttgc accgcgccgt cgtagacata ggcccgcagg
     2221 tcgaacttca tcgcttcggc atgtcgcgcg ggcaggcgct gacccggcgc cacgatcgcc
     2281 tgtgcgacat agtcgccttc gcaaatttcc ttccacacgc gccgagtgat cttgtcgccg
     2341 cgataggccg ccttgctgcc gaatccggct cgcggcttga agaacaggtc gcgccgacgc
     2401 gcccaaagcg attctgcctg ttccgcggtg acgcgttcgg tgcgcgggat gccggcggcg
     2461 aggacttccc gcacgtcccg tgccacgccg agatgctcga gcgcctgcgg gtcggacagc
     2521 agcgcgagat tggccttgtc cgcgtacagc gcatgcgcgc gcggatgggg cgtgatcacg
     2581 gccgcatccg cgaaccatgc ggcgcgcagg gccgcgctcg cagggttttc gaatgcaaag
     2641 tcggtgctgc ggttgtagac gaggtcgatc ggctggtcgt cgtagcagag ccggccgcta
     2701 tcgaagcgca gccgggccgg atcggccacg accgctgtga tcccgttgcg gcggaacagt
     2761 tcgcgaaaca gcaggaattc cgggtacagg tactggttct gcggatcgtg atcgacgatc
     2821 gcgacgcttt gcagcgcacc ggcacggccg gcgaggcgcc actcgttgcg gaacatgtcg
     2881 agcatgcgct gctcggcggc ttctgcttcg acaaccggga acagtgcctc caccggcgcg
     2941 cagcagccgc gctgcgccgc gccgagtacg gcattgagca gcgcgccccc ggcattggta
     3001 ttgatttcga tgagtccgat gcggtcgagg cccacgtgaa agtcgtagcc gaagaatacg
     3061 ctgcgggctg gcgtttcatg gcgtgccgca accggcgagc gcgcgagcac ctgttcctgc
     3121 cacgcgggca atgccaccac atgctcgact gcggccacga tttcgcgcat gcgtgcgata
     3181 tggccggcct cgacgaatac cggatgcgcg gagaacaggt gcggacagcg ttcgtcgacc
     3241 agttcggcaa ggcccggaac gccttgctgt gccgcgaatg catgccggac cgcggaccgg
     3301 tccaggccga tgcagaagca cccgttgttg agcgcgctcg catgcatccc gcagccaacg
     3361 ctgcgctcga acggcaggat ggtcgcgtcg ttcatctcag tgggccgcct cgtaccaggt
     3421 gcgcgtgcga ttgaccatgc gcaccagcgc aagcatcacc ggcacctcga tcagcacgcc
     3481 gaccacggtg gccagcgccg cgccggagtc gaagccgtac agcacgatcg ccaccgccac
     3541 cgccagttcg aagaagttcg atgcgccgat catcgtcgag ggtccggcca cgctatgcgc
     3601 aacccgcatc ttcctgttga gccagtagcc gagcgcggcg atgaacagca cctgcagcag
     3661 gatcggcacc gcaaggatcg cgatgatcag cggctgctcg atgatctggc ggccctggaa
     3721 cgcgaacaac agcaccagcg tggcgagcag ggcgacgatg gaaaaaggcg tgacgcgcgc
     3781 gagcgccgcc tcgaacacgc cggacccgcg cgcccgaagc cggccgcgca ggaactgcgc
     3841 gatcgccagc gggatcgcga tgtacatcac gaccgacagc agcaaggtat cccagggtac
     3901 cgggatcgat gacacgccga gcaacagcgc gacgatgggc gcaaacgcga acagcatcac
     3961 cacatcgttg agcgccacct gcgtgatcgt gaagttggcg tctccgcggc acaggttgct
     4021 ccatacgaag accatcgcgg tgcacggcgc ggcgccaagc aggatcaggc cggcgatata
     4081 gctgtcgatc tgcgcggccg gcagccaggg cgcgaacaca tggcgcagga aaaaccagcc
     4141 gaacaaggcc atcgagaacg gcttgaccgc ccagttcacg aacagggtga tgccgacgcc
     4201 ggcctcttgc ctgcgcacct cgtgcagcga ggaaaagtcg atcttcatca gcatcggcac
     4261 gatcatcgcc cagatcagga tgccgaccgg gatgttgacg tgggc
//
LOCUS       DATLGF010000373         3595 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_140372, whole genome shotgun sequence.
ACCESSION   DATLGF010000373 DATLGF010000000
VERSION     DATLGF010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140372"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..950)
                     /locus_tag="VM406_11510"
     CDS             complement(57..950)
                     /locus_tag="VM406_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVL76632.1"
                     /translation="MLSTQLPENRSETSPSVSLHSTSHEAGWQRQGKLWSNLKELCDV
                     LRIPSAAIHTDEELLFQHVQFKTGQRVHTIGQPFDTLYIVHSGFLKTVLIDEFGNEQV
                     LSFPMKGDLFGVDGIHTKRYASEAVALSNCDLILLPFKKLTQLGRAHSEFEHAMYSVM
                     SRELVREQAMVSMLGALSAEARVARFLVSLSERYAEMGYSSKLFNLRMTRHEIGSYLG
                     LTLETVSRTLSAFNEIGLITVDQRSIGIKDLEALRTLRRLPPSSTRARQLASKKARQA
                     AAAKSPQENQENGDWGTLASA"
     gene            complement(1262..1906)
                     /locus_tag="VM406_11515"
     CDS             complement(1262..1906)
                     /locus_tag="VM406_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL76633.1"
                     /translation="MIRIVIADDHTIMREGLKRILEGADDIEVVGEAVDGFEALAHVR
                     KGGFDLLMLDLSMPGRSGVELIRQIKDEVPKLPILILTMHEEEQYAVRAIRAGARGYL
                     TKESAGTQLVSAIRKVASGRPYISLEVAEQLAMDVMPANEDLPHKQLSNREFEVFTLL
                     VSGKSITDIADFLHLSAKTVSTHKTRILTKMNMTSLAEMVQYAVQHRLLGPLKS"
     gene            complement(1884..3065)
                     /locus_tag="VM406_11520"
     CDS             complement(1884..3065)
                     /locus_tag="VM406_11520"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HVL76634.1"
                     /translation="MNFQPDHGAAMRSNPITMLPRFPAVMLGASLLEMIQSSSDGVVV
                     IDAARQIVLINHRAERIFGYPANLLLEKPLDLLIPACSDTDSRRRLDRLGSLRSCGRR
                     TRMVLRGVRADGALLDLKTSVTRVGIRGDMFLCLVFQEPAALDTAPCGSTAIALGPGR
                     QWAVSSHQASEVEKRRFSRKLYDDIGQRLSVLKLDLSWLENSLPHTNQYLPARLAEMQ
                     GLLDNVITLTKSMASTLRPPVLDDFGLVPAVEWMVESLRKRTGIRCTLESEGLVGKLD
                     EALESAAFRIVQEGLANIEQHSGAEHAWVSLRVADQRIRIDIRDDGVGLKNPEPVKTG
                     CYGLLAMYQRVFILGGTIRIENARPHGVLLQANIPLDPAFAGDPNPQSPWQAYDSHRH
                     R"
     gene            3326..>3595
                     /locus_tag="VM406_11525"
     CDS             3326..>3595
                     /locus_tag="VM406_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HVL76635.1"
                     /translation="MDLQLGNIGNTIQLAIAPVFLLTGVGTNLAVLTSRLARIIDRSR
                     VLEDRLAADGGTPPSAAVADELHALYQRASLINRAITLSTACGLLV"
BASE COUNT          650 a         1072 c         1157 g          716 t
ORIGIN      
        1 cttcccttcc atgcaacggc ccgcgcgggc gggccgttgc atggaaggga aggggctcag
       61 gcggatgcga gtgtgcccca gtccccgttt tcctgattct cctgcggcga cttcgctgcg
      121 gcagcctggc gggccttctt cgatgcgagc tggcgggcgc gagtgctgga aggcggcagg
      181 cgacgcaggg tgcgcagcgc ctccaggtcc ttgatgccga tcgaacgctg gtccacggtg
      241 atcaggccga tctcgttgaa ggcggaaagc gtgcggctga cggtctccag cgtcaggccg
      301 agatagctgc cgatttcgtg gcgcgtcatc cgcaggttga acagcttgct cgaataaccc
      361 atctcggcgt agcgctcgga caggctgacc aggaaacgcg ccacgcgcgc ctccgcactg
      421 agtgcgccca gcatgctgac catggcttgc tcgcgcacga gctcgcggct catcacgctg
      481 tacatcgcgt gttcgaactc cgaatgggcg cggcccagtt gcgtcagctt cttgaacggc
      541 agcaggatga ggtcgcagtt cgacagcgcc acagcctcgg acgcatagcg cttcgtatgg
      601 atgccgtcga cgccgaacag atcgcccttc atcggaaagg agagcacctg ctcattgccg
      661 aactcgtcga tcaggacggt cttcaggaac ccggagtgga cgatgtagag ggtgtcgaaa
      721 ggctggccga tggtgtggac gcgctgaccg gtcttgaatt ggacgtgctg gaaaagaagt
      781 tcttcgtcgg tgtggattgc ggcagagggg atacgcagga cgtcgcacag ttctttcaga
      841 ttggaccaga gttttccctg gcgctgccaa ccggcttcat gcgaggtgga atgcaaactg
      901 acggatggtg atgtttcgga gcgattctca ggcaattgcg tggacaacat atgcatctcc
      961 taatccagta acgttcgggc ggacaacgtg cggcggcttg cggcgccgta cgatcgcgac
     1021 gtgcgcgtct ccgttgtttt tgtgggtgcg gctggtgttg cgaaaacctt gcgcgaccgg
     1081 gcgcggcaaa gttgccgcgg ctcacagccg tgctgtgaaa gcggtcagta tcgcaaccga
     1141 gaggagggga tggctatcgg gagccgctga attgaggtgt aggtcgccga caccggcgat
     1201 aggaatattc ctacactttt tgctccgttt ttccgaaatg atgcggtttc ggaccggcgt
     1261 gtcagctctt gagcgggccg agaaggcgat gctgtaccgc gtactgcacc atttctgcca
     1321 gtgaggtcat gttcatcttg gtcaggatgc gcgtcttgtg cgtgctgacg gtcttggcgg
     1381 agaggtggag gaagtcggcg atatcggtaa tcgacttgcc cgacaccagc agcgtgaaga
     1441 cttcgaactc gcggttcgac aactgcttgt gcggcaggtc ctcgttggcg ggcatcacgt
     1501 ccatcgccag ctgctcggcc acttccaggc tgatgtacgg ccgcccggag gcgaccttcc
     1561 tgattgcgct caccagctgc gtgcccgcgc tttccttcgt cagatagccg cgcgcgccgg
     1621 cccggatcgc ccgcaccgcg tactgctctt cctcgtgcat cgtgagtatg aggatgggca
     1681 gcttcggcac ttcatccttg atctggcgga tcagttccac gccgctgcgg ccgggcatgg
     1741 agagatcgag catcaggagg tcgaagcctc ccttgcgcac gtgcgcgagt gcttcaaaac
     1801 catccaccgc ttcgccgacg acttcaatat cgtcggcgcc ttcgaggatg cgcttgaggc
     1861 cttcacgcat gatggtgtga tcatcagcga tgacgatgcg aatcataggc ttgccatggc
     1921 gactgtgggt tgggatcccc cgcaaaggcg ggatcaaggg gaatgttcgc ttgcagcagc
     1981 accccgtggg gacgtgcgtt ttcgatgcgg attgtacctc ccaaaatgaa aacgcgttga
     2041 tacatggcta gtaaaccgta gcaaccagtc ttgaccggtt ccggattttt caggccgaca
     2101 ccgtcatcgc ggatgtcgat gcggatgcgc tgatcggcaa cgcgcagcga gacccaggcg
     2161 tgttcggctc ccgagtgctg ctcgatgttg gccaatcctt cctggacgat gcgaaacgcc
     2221 gcggactcca gcgcctcgtc gagtttgccg accagacctt cgctttccag cgtgcagcgg
     2281 attccggtgc gcttgcgcag gctttccacc atccactcga ccgccggaac gagaccgaaa
     2341 tcatcgagca ccggcgggcg cagtgtggac gccatgctct ttgtcagcgt gatgacgttg
     2401 tcgagcagac cctgcatttc ggccaggcgc gccggcagat actgattggt gtgcggcagg
     2461 ctgttttcca accagctcag gtccagcttc agcacgctca gtcgctgtcc gatatcgtca
     2521 tagagcttgc gcgagaagcg gcgcttctcc acttccgaag cctgatgcga agagactgcc
     2581 cactgccgcc ccgggccgag ggcaattgca gtgctgccgc atggcgccgt gtccaacgcc
     2641 gcgggctcct ggaacaccag gcacaggaac atgtcaccac gtattccgac gcgggtcacg
     2701 ctcgtcttca gatccagaag ggctccgtcc gcgcgcacgc cgcgaagcac catgcgggtg
     2761 cgacgtccgc aggaacgcag agagccgagg cggtcgaggc gtcggcgcga gtcggtgtcg
     2821 gagcacgcgg ggatcagcag gtcaagcggt ttttccagca ggagattggc cggatagcca
     2881 aaaatgcgtt cggcccggtg attgatcagc acgatctggc gcgccgcatc gatcaccaca
     2941 acgccatcgg aagaggactg gatcatctcg agcaacgacg cgcccagcat gaccgcagga
     3001 aagcgcggaa gcatggtgat cgggttcgag cgcattgcag cgccatgatc cggttggaag
     3061 ttcatttcgg tttccggtac gcgtcgagcc atcttggctc cggtaccgcc aggttgaggc
     3121 cggcagaatc gcaggtcaca tgttagacgg ctgatgcggc gcgcgcttga tcgtatcggc
     3181 cctcgccgac cgcgcggctt gagatttgcg gaatgtgcgg cgaccgccgt gctggcgccg
     3241 actgccggcg gaggcacctg tcgaacggct gctgttagac taccggcctc caatgcgata
     3301 cggctggaag acagggaggt gccacgtgga cctacaactt ggaaacatag ggaataccat
     3361 ccagcttgcc attgcgccgg tgttcctgct caccggcgtg gggaccaatc ttgccgttct
     3421 gaccagtcgc ctggctcgca tcatcgatcg ttctcgcgta ctggaggatc gcctcgcggc
     3481 cgacggggga acgccgcctt ctgccgctgt cgccgacgag ctgcatgcac tgtatcagcg
     3541 cgcatcgctc atcaatcgcg ccattaccct gagcaccgcg tgcggcctcc tggtc
//
LOCUS       DATLGF010000374         3601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_94179,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000374 DATLGF010000000
VERSION     DATLGF010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94179"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1882)
                     /gene="dxs"
                     /locus_tag="VM406_11530"
     CDS             complement(<1..1882)
                     /gene="dxs"
                     /locus_tag="VM406_11530"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HVL76636.1"
                     /translation="MSMNLLNTINLPADLRRLPRHQLKPLADELRAFVLDSVSKTGGH
                     LSSNLGTVELTIALHYVFNTPDDRIVWDVGHQTYPHKILTGRRERMHTLRQLNGISGF
                     PRRDESEYDTFGTAHSSTSISAALGMALAARTKGEDRHAIAVIGDGAMTAGMAFEALN
                     NAGVHTDTKLLVILNDNDMSISPPVGALNRYLAQLMSGRFYAAAKNVGKNVLKVAPPL
                     FELAKRLEEHAKGMVVPATLFEQFGFNYIGPIDGHDLESLVPTLENIRQLRGPQFLHV
                     VTRKGQGYKLAEADPVAYHGPGKFDPKVGLVAPATPPRPTFTQVFGQWLCDMAARDRR
                     LVGITPAMREGSGLVEFEKRFPDRYHDVGIAEQHAVTFAAGLACEGLKPVVAIYSTFL
                     QRAYDQLVHDVALQKLPVVFALDRAGIVGADGATHAGVFDIPFVRCIPNMSMACPADE
                     NECRALLTTAFEQDHPVAVRYPRGAGVGAKVSTGLEGLPFGKGEVRRKGRGVAILAFG
                     TLLHPALEAAERLGATVVNMRWAKPLDEVLLLEVATAHEAIVTLEEGAIMGGAGSAVS
                     EALQANGLARPVLHLGLRDEFTTHGDPARLLALHGLDAAGIERSVRERFAQLVTPTAP
                     GLK"
     gene            complement(1923..2819)
                     /locus_tag="VM406_11535"
     CDS             complement(1923..2819)
                     /locus_tag="VM406_11535"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HVL76637.1"
                     /translation="MSAPAFVDWMQGVQSTMEAALGEFLPADGTAPQQLHAAMRYATL
                     DGGKRVRPLLVFAAGDLFGAPAHLLARAGAAVEMIHAYSLVHDDMPCMDDDALRRGKP
                     TVHVKYGQATALLVGDALQSQAFELLADGSGDLDPARRLNMVQVLARASGSLGMCGGQ
                     AIDLASVGRQLSLDALEQMHRLKTGALLRASVLLGALCGSRLDADQHAALDAYSAAIG
                     LAFQVVDDVLDATADSATLGKTAGKDAADNKPTYVSILGLDASRELAQKLRADAHAAL
                     APFGDPARRLREIADLIVQRKA"
     gene            complement(2816..3067)
                     /locus_tag="VM406_11540"
     CDS             complement(2816..3067)
                     /locus_tag="VM406_11540"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HVL76638.1"
                     /translation="MPTKPAPSDQPVSFEAAMAELEQLVARMEAGELPLEASVAAYKR
                     GTELVRYCAGQLDKVEQQVKVLEGEMLKPFAAEPAGDEA"
     gene            3402..>3601
                     /locus_tag="VM406_11545"
     CDS             3402..>3601
                     /locus_tag="VM406_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HVL76639.1"
                     /translation="MSDLANFAKLARSNAQLPVNVYFDETLLQKEIEQLFQQGPRYAG
                     HELQVPNPGDFATLSWENEGRML"
BASE COUNT          581 a         1189 c         1218 g          613 t
ORIGIN      
        1 ccttgagccc cggcgcggtg ggcgtcacga gctgcgcgaa gcgctcgcgc accgaacgtt
       61 cgatgcccgc ggcgtccagc ccgtgcagcg ccagcaggcg ggcaggatcg ccgtgcgtgg
      121 tgaattcgtc gcgcaggccc aggtgcagca cgggcctggc gaggccattg gcctggagcg
      181 cttccgagac ggcgctgccg gcgccgccca tgatggcccc ctcctccagc gtcacgatgg
      241 cttcgtgggc cgtcgcgacc tccagcagca gcacttcgtc gagcggcttg gcccagcgca
      301 tgttgaccac cgtcgcaccc aggcgttcgg ccgcttcgag cgcgggatgg agcaaggtgc
      361 cgaaggccag gatcgcgacg ccgcggccct tgcgccggac ctcgcccttg ccgaagggca
      421 gcccctccag gccggtcgag accttcgcgc ccacgccggc gccgcgcggg tagcgcacgg
      481 ccaccggatg gtcctgctcg aaggccgtgg tgagcagcgc gcggcattcg ttctcgtcgg
      541 ccgggcaggc catgctcatg ttcggaatgc agcgcacgaa gggaatgtcg aacacgccgg
      601 cgtgcgtggc gccgtccgcg ccgacgatgc ccgcgcggtc gagcgcgaac accaccggca
      661 gcttctgcag ggccacgtcg tggaccaact ggtcgtaggc gcgctgcagg aaggtcgagt
      721 agatcgccac caccggcttg agcccctcgc aggccaggcc ggcggcgaag gtgacggcgt
      781 gctgctcggc gatgccgacg tcgtggtagc ggtcggggaa gcgcttctcg aattcgacca
      841 gccccgagcc ttcgcgcatg gcgggcgtga tgcccaccag ccgccggtcc ctggccgcca
      901 tgtcgcacag ccactggccg aagacctgcg tgaacgtggg cctgggcggc gtggccggcg
      961 cgaccaggcc taccttgggg tcgaacttgc ccgggccgtg gtaggccacc ggatcggcct
     1021 cggcgagctt gtagccctgg cccttgcgcg tgaccacgtg caggaactgc ggcccgcgca
     1081 gctggcggat gttctccagc gtgggcacca gcgattcgag gtcgtggccg tcgatcgggc
     1141 cgatgtagtt gaagccgaac tgctcgaaca gcgtggccgg caccaccatg cccttggcgt
     1201 gctcctccag ccgcttggcc agctcgaaca gcggcggcgc caccttgagc acgttcttgc
     1261 cgacgttctt ggccgccgcg tagaagcggc cgctcatgag ctgcgccagg tagcgattca
     1321 gcgcgcccac cggcgggctg atcgacatgt cgttgtcgtt caggatcacc agcagcttgg
     1381 tgtcggtatg gacaccggcg ttattcaagg cctcgaaggc catgccggcg gtcatcgcgc
     1441 cgtcgccgat gacggcgatc gcatgccggt cctcgccctt ggtccgggcg gccagcgcca
     1501 tgccgagcgc ggccgaaatc gaggtcgacg aatgcgcggt gccgaaggtg tcgtattcgc
     1561 tctcgtcacg gcgcggaaag ccggagatgc cattcagctg gcgcagcgtg tgcatgcgct
     1621 cgcgccggcc ggtcaggatc ttgtgcggat aggtctggtg gccgacatcc cagacgatgc
     1681 ggtcgtccgg cgtgttgaac acgtaatgca gcgcgatcgt cagctccacc gtcccgaggt
     1741 tcgacgacag gtggccgccg gtcttggaga ccgagtcgag cacgaatgcg cgcagctcgt
     1801 cggccagcgg cttcaactga tgacgcggca ggcggcgcag atcggcgggc aggttgatgg
     1861 tgttcagcag attcatactc atgatcaacg ggcgttagcc gctttcctgt ggtcggcctc
     1921 gttcaggcct tgcgttgcac gatcaggtcg gcgatctcgc gcagcctgcg cgccggatcg
     1981 ccgaaagggg ccagcgcggc gtgcgcatcg gcgcgcagct tctgcgccag ttcgcgcgac
     2041 gcgtcgaggc cgagaatcga cacataggtc ggcttgttgt cggcggcgtc cttgccggcg
     2101 gtcttgccga gcgtggctga atcggcggtc gcgtcgagca cgtcgtcgac gacctggaac
     2161 gccagtccga tcgccgccga ataggcatcg agcgcggcat gctggtccgc atcgaggcgg
     2221 ctgccgcaca acgcgccgag cagcaccgac gcgcgcagca atgcaccggt cttcaggcga
     2281 tgcatctgct cgagcgcatc gaggctcaac tggcgaccca cgctggccag atcgatcgcc
     2341 tggccgccgc acatgccgag cgaaccggat gcgcgcgcca gtacctggac catgttcagc
     2401 cggcgcgccg gatcgaggtc gccggagccg tcggcaagca gttcgaacgc ctgggactgc
     2461 agcgcatcgc cgaccagcag cgccgtcgct tgtccatact tcacgtgcac cgtcggcttg
     2521 ccgcgccgca gcgcgtcgtc gtccatgcag ggcatgtcgt cgtgcacgag cgagtacgcg
     2581 tggatcattt cgaccgcggc gccggcccgc gcgagcaagt gcgcaggtgc gccgaacagg
     2641 tcgccggcgg cgaacaccag cagcggccgc acccgcttgc cgccatcgag cgtcgcatag
     2701 cgcatcgccg cgtgcagctg ctgcggcgcc gtgccgtccg ccggcagaaa ttcgccaagc
     2761 gccgcctcca tcgtcgactg gacgccctgc atccagtcga caaatgcggg cgcactcatg
     2821 cctcgtctcc tgccggctcg gccgcgaacg gcttgagcat ctccccttcc agtaccttca
     2881 cctgctgctc caccttgtcg agctgaccag cgcaatagcg cacgagctcg gtgccgcgct
     2941 tgtaggcggc cacggaagcc tcgagcggca gctcgccggc ttccatgcgc gcgaccagct
     3001 gctcgagttc ggccatcgcg gcttcgaacg agacgggctg gtctgagggt gcgggcttgg
     3061 tgggcataga ggggaagaaa aatggagaag tttaacccgt tattctagaa caaaccattt
     3121 gcaggccatg ccggcccgct tgactggcag caagcttcga cacgcatgtt gacggaggtc
     3181 atgcagcggc caaagatagt gaaaagcgct tttcatcgcg ttcggtccgg ggcaagatca
     3241 gcagggaaag ggctcgaagt ggggcgtttt ccggtcttta gagcctgtcg ctggggtata
     3301 atcgcccgtt ctgtccaaaa tttccgtttc atctcatctg agtgccggtt tttgcggatt
     3361 ctttctctct taggttggtg caaattaatt tgggggcggg gatgtccgat ctggctaatt
     3421 ttgccaagct ggcgcgttcg aacgcgcaac ttccggtcaa cgtctacttc gacgagacac
     3481 tgctgcagaa ggaaatcgag cagctattcc aacaaggacc gcgctacgcc ggtcacgaac
     3541 tgcaggtgcc gaatcccggc gactttgcaa ccctttcatg ggaaaacgag ggccgcatgc
     3601 t
//
LOCUS       DATLGF010000375         6106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_74616,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000375 DATLGF010000000
VERSION     DATLGF010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74616"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VM406_11550"
     CDS             complement(<1..282)
                     /locus_tag="VM406_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76640.1"
                     /translation="MADQNKGGTSPPGTPAAMPGSSGSFGSIGKGSSGSDASARGGMD
                     EGARQAVQHTKDEARHVAQEMSEGAKEKGRSLFEQQKESAARQMGSVAHA"
     gene            513..887
                     /locus_tag="VM406_11555"
     CDS             513..887
                     /locus_tag="VM406_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76641.1"
                     /translation="MRKLTRIIALALPLLPWAGASQASDVRVGVTVAGQVSPGVYGRI
                     EIGNQPPPPVVYTKPVVIVQQPTPVYAAPVYLHVPPGHAKNWGKHCHKYNACAQPVYF
                     VKSAEYEQPRGKGKDGHRGRRD"
     gene            complement(928..2172)
                     /locus_tag="VM406_11560"
     CDS             complement(928..2172)
                     /locus_tag="VM406_11560"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HVL76642.1"
                     /translation="MLRQGGLGVPLQHRHAAHTPPSRRVRAKTRADGVTWLAKTSSLC
                     VATRHHQHAFWRERIPRIECQQPLVLGANGRFGRVAVETFAGAGWRVLAQSRKPPVAA
                     LPAQATAILCDATDTEGILAACPAPVDAVIHALSPAYTDAAWQREVMALGDSAIALAR
                     AKGALLMLPGNVYNFGKRLPPLLSERTRQVPDTPKAALRIALEQRLQAVAGEGVRSVV
                     IRAGDFIGAEPGTWLDLGVARQLHKGRYTCMGPQGLVHAWAYLPDLAQVFVAVAQQRE
                     KLQAFEVFHYGGLCMTGAQLHAALERVTGGTLVRVRFPWWMLRVAAPFSPMMRAVLEM
                     RYLWGRSHCLSQQRLEALLGKVPCTSLDAVLRKVLVAPQYKLPASARAVSSGAEAPTP
                     GHSRTPVPSAPDARGWETPRSR"
     gene            2363..3307
                     /locus_tag="VM406_11565"
     CDS             2363..3307
                     /locus_tag="VM406_11565"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL76643.1"
                     /translation="MHTSKSGGGAVDAHALDWNLLKCFCAVAEAGAVSRAAALLGVSQ
                     PTVSRQIAELESAVGAALFERVARGVALTSAGVALREPARRMLEAAQAASVAAAAQGT
                     ELAGTVRITASETMAAFVLPPMLARLRAAHPRIQIELVASNRLDNLLTREADIAVRMM
                     RPAQSGVVTRRIAAYPVGFYAHRDYLKTRRLPADGADPRRYDWVGLDQSNMMIEGFRA
                     AGYMVDKSFFAFRCDNHIVGWQAVLAGMGVGVTLQCVGERYRELVRVLPDQKLPPLEV
                     WLAAHRELRDVPRIRTVFDLLAAELARGELKAPAGAPG"
     gene            complement(3279..5585)
                     /locus_tag="VM406_11570"
     CDS             complement(3279..5585)
                     /locus_tag="VM406_11570"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL76644.1"
                     /translation="MFELANASFLKVARRSDVIGKPVRETLPESVSLGFVDLLDEVYR
                     SGKPFVGRGLRHVVRDDAGVASEGYVDFVYQPITDSAGRVIGIFIQGHDVTEASHAHA
                     ALREAEERLQLALEAGGMGAWHADLRTGKSEWLGGMATLHGRSADGYAHSVQAYGELV
                     HPADRQSVIDAVNRALQERRDHRIEYRIVWPDGSVRWLEGRGRIYLDEQGEPARMAGV
                     CMDITARKIAEQDLHFLAQASMELAHLVDVPATLDRLAKLAVPTFADWCAVDVLEDDG
                     TLRRLAVAHCDPRKEALAHELHRRYPPAPDAGIGVWNIMRTGAPELVRELSDDVLATA
                     ASDAEHLEIVRGLGLRSYIGVPLKIKGQPFGVVSFVSAESGRLYGPADLALAEDLALR
                     TSIAVENARLYQAVQEADRRKDVFLATLSHELRNPLAPIASALALLRVAPHDAGRVDR
                     SVRMMERQVSQLSRLVDDLMDVSRITAGKMEMRRERASLSSILHNALETSRPHIEQAR
                     HQLLVNLPGMPMEVDADPVRLAQVFSNLLNNAAKYTPPSGRIELTAAREGNGFLVRVR
                     DNGIGIPREMLRSVFSMFAQERRPSEYSRGGLGIGLSLAEGIVVLHGGRIEAHSEGNG
                     RGSEFHVWLPRAAGADAASEAAPPGTAARQGMRILVVDDNVDAADTVSELLRLLGHEV
                     SVVHSGEEALEAAPGMEPAIVLLDIGLPGIDGYETARRLRALPGLAAARLIALTGWGQ
                     DQDKARTAAAGFEQHWVKPVSLALLQAL"
     gene            complement(5467..>6106)
                     /locus_tag="VM406_11575"
     CDS             complement(5467..>6106)
                     /locus_tag="VM406_11575"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HVL76645.1"
                     /translation="ALMRERDWASTPLGPPSGWSPGLRMVVRLVLDAHFPMYVAWGPQ
                     LVLIYNDAYAELLGARHPAALGRAFWDIWPEVKGEIGPVIRTALGGETVYIENQPLTL
                     LRYGMQEHAWFTFSVSPARDESGATAGVYCVCTETTASVLLERERVEENQRLLRLFEQ
                     APGLHGGRARPGSRVRAGQCQLPQGCPAQRRDRQACARNPSRKRESRFRRSA"
BASE COUNT          979 a         2127 c         2034 g          966 t
ORIGIN      
        1 ggcatgggct accgacccca tctgacgcgc cgccgactcc ttctgctgct cgaacagcga
       61 acgtcccttt tccttcgccc cttcgctcat ctcttgcgcg acatgccggg cctcgtcctt
      121 cgtatgctgc accgcctggc gggcgccctc gtccatcccg ccgcgcgcgc tggcgtcgct
      181 gccgctgctg cccttgccga tggatccgaa cgatccgctg ctgccgggca ttgctgccgg
      241 tgttcccggc gggctggtcc cgcccttgtt ctggtctgcc atgagtcaat ctccttgcgt
      301 tggtgatgat gtcgcgcgag gaaacgccga ctgccgcatc gctgacgtct gcgctcgctc
      361 ccatccgggt gaggtttcga atgcaagatg cagaccaata ggctgaaaga aagacaagag
      421 ccttcgcagt agtaacgaaa agcaatgcct gatgcgcaat gaggggcact ggagtatgct
      481 ctggaacctg tttcggcctg accggagaat ccatgcgcaa gctcacaaga atcatcgccc
      541 tcgccttgcc cctgctgccc tgggcaggcg cctcgcaggc ttccgacgtg cgtgtcggcg
      601 tcactgttgc cggccaggta tcgcccggcg tctacgggcg gatcgagatc ggcaaccagc
      661 cgcccccgcc cgtggtgtat acgaagccgg tcgtgatcgt gcagcagccc actcctgtgt
      721 atgcggcgcc ggtctacctg catgtgccgc ccggacatgc gaaaaattgg ggcaagcact
      781 gccacaagta caacgcctgc gcccagccgg tgtatttcgt gaaatcggcc gaatacgagc
      841 agccgcgcgg caagggcaag gacggccacc gaggccggcg cgactgaccc gcaccgccgg
      901 gcatcgaaca ggaggatggg ccgccgctca gcgtgagcgc ggtgtttccc acccgcgagc
      961 atccggcgcc gagggcacag gtgtacgcga atgccccggc gtaggcgctt cggcgccgga
     1021 cgacacggcg cgcgccgagg ccggcagctt gtactgcggc gccaccagca ccttgcgcag
     1081 caccgcgtcg aggctggtgc acggcacctt gcccagcaag gcctccaggc gttgctgcga
     1141 caggcagtgc gaacgtcccc acagatagcg catctccagc accgcgcgca tcatcggcga
     1201 gaacggcgcg gccacgcgca gcatccacca cggaaagcgc acgcgcacga gcgtgccgcc
     1261 ggtcacccgc tccagcgccg catgcagttg cgcgccggtc atgcacagcc cgccgtagtg
     1321 aaacacctcg aaggcttgta gcttctcgcg ctgttgcgcc acggcgacga acacctgcgc
     1381 cagatcgggc aggtacgccc acgcatgcac gagtccctgc ggccccatgc aggtatagcg
     1441 gcccttgtgc aactgccgcg caacaccgag atccagccag gtacccggct cggcaccgat
     1501 gaagtcgccg gcgcggatga ccacgctgcg caccccttcc cccgcgaccg cctgcaggcg
     1561 ctgctcgaga gcaatgcgca gcgccgcctt cggcgtgtcc gggacctggc gcgtgcgctc
     1621 cgaaagcaac ggcggcagcc gtttgccgaa gttgtacacg ttccccggca gcatcagcag
     1681 cgcgcccttc gcgcgtgcga gcgcgatcgc gctgtctccg agcgccatca cctcgcgctg
     1741 ccaagcggcg tcggtgtagg cggggctcaa ggcatggatg acggcatcca ccggtgcggg
     1801 acaggcggcg agaatgcctt cggtgtcggt ggcgtcgcac aggatggccg tcgcctgcgc
     1861 cggcagcgcg gcaaccggcg gcttgcgcga ctgtgcgagg acgcgccaac cggcgccggc
     1921 gaaggtttcc acggcgacgc ggccgaagcg gccgtttgcg ccgaggacaa ggggctgttg
     1981 acactcaatt cgagggatgc gttcgcgcca aaacgcgtgc tggtggtggc gcgtggcgac
     2041 gcaaagactg gacgtctttg ctagccatgt aacgccgtca gcgcgcgttt tggcacgaac
     2101 cctccgggaa gggggcgtat gcgccgcatg gcggtgttgc agggggacgc cgagtcctcc
     2161 ttgccgtagc accgctacgt cgcgtcgctg cgcctagccc tgcgacgcat acgacccctt
     2221 cgcacccccg aattgagtgt caacagcccc tacggtggat ctggcggatt ccatgcgact
     2281 ctccaatgaa cggcgatgcg gccattgtgg gcgcctgaca tctgcataac aatacgcaga
     2341 cttgcattcc acctattcaa atatgcatac ctcgaaatcc ggcgggggcg ctgtcgacgc
     2401 ccatgcgctc gactggaacc tgctcaagtg tttctgtgcg gtagcggaag ccggcgcggt
     2461 ctcgcgcgcg gcggcgctgc tgggcgtctc gcagccgacc gtgtcacgtc agatcgcgga
     2521 actggaaagc gcggtcggcg ccgctctgtt cgagcgggtg gcgcgcggcg tggcgctgac
     2581 gtcggcgggc gtcgcactgc gcgagccggc gcgacggatg ctggaggcgg cccaggcagc
     2641 gagcgtggcg gcggccgcgc aaggcacgga gctggccggc accgtgcgca ttaccgccag
     2701 cgagaccatg gctgccttcg tgctgccgcc gatgctggcc cggctgcgcg cggcccatcc
     2761 gcgcatacag atcgaactgg tggcttcgaa ccgtctggac aacctgctca cgcgcgaggc
     2821 cgatatcgcg gtgcggatga tgcggccggc gcaaagcggc gtggtcacgc gacgcatcgc
     2881 ggcctacccg gtcggattct atgcgcaccg cgattacctg aagacgcgcc ggcttcccgc
     2941 cgacggcgca gacccgcggc gctacgactg ggtcgggctg gaccagtcga acatgatgat
     3001 tgaaggcttc cgcgcggccg ggtacatggt ggacaagagc tttttcgctt tccgctgcga
     3061 caaccacatc gtcggctggc aggcggtgct ggcgggaatg ggtgtgggcg tgacgctgca
     3121 atgcgtgggc gagcgctacc gggaactggt gcgcgtgctg ccggaccaga agctgccgcc
     3181 cctggaagtc tggctggcgg cgcatcgcga gctgcgcgac gtgccgcgca tccgtacggt
     3241 gttcgacctg ctggcggcgg agctggcgcg cggcgagctc aaagcgcctg caggagcgcc
     3301 aggctgaccg gcttgaccca gtgctgctcg aagcctgctg ccgcagtgcg cgccttgtcc
     3361 tggtcctgtc cccagccggt caatgcgatg agccgggcgg cggccaaccc gggcagcgcg
     3421 cgcaggcgcc gcgcggtctc atagccatcg atcccgggca ggccgatgtc gagcagcacg
     3481 atcgccggct ccatgcccgg cgccgcctcc agcgcctcct cgccgctatg cacgacgctg
     3541 acctcgtgcc ccagcaggcg cagcaattcg gaaaccgtgt cggcggcatc gacattgtcg
     3601 tccaccacca ggatgcgcat cccctgccgc gccgccgtgc ccgggggcgc ggcttccgac
     3661 gccgcgtccg ctccggccgc tcgcggcaac cagacatgaa actcgctgcc gcgcccgttg
     3721 ccttcgctat gcgcttcgat tcgtccgccg tgcagcacga cgataccctc ggccagcgac
     3781 aggccgatgc ccagcccgcc cctgctgtac tcgctcggcc tgcgctcctg cgcgaacatc
     3841 gagaacaccg agcgcagcat ctcgcgcggt atgccgatcc cgttgtcgcg aacccgcacg
     3901 aggaagccgt tcccttcgcg cgcggccgtc agctcgatgc ggcccgacgg cggcgtgtat
     3961 ttcgctgcgt tgttcaggag gttcgagaac acctgggcca ggcgtaccgg atcggcatcc
     4021 acttccatcg gcatgccggg caggttgacg agcagctggt gacgcgcctg ttcgatatgc
     4081 ggacgactgg tctcgagcgc gttgtgcagg atagacgaca ggctcgcgcg ctcgcggcgc
     4141 atctccatct tgccggcagt gatgcgcgag acgtccatca ggtcgtcgac cagccgcgag
     4201 agctgggaca cctggcgctc catcatgcgc accgagcggt ccacgcggcc cgcgtcgtgc
     4261 ggcgccacgc gcagcagcgc cagcgcgctg gcgatcggcg cgagcggatt gcgcaattcg
     4321 tgcgacagcg tggccaggaa cacgtccttg cgccggtccg cctcctgcac cgcctggtac
     4381 aggcgcgcgt tctcgaccgc gatcgaggtg cgcagcgcga ggtcttcggc cagtgccagg
     4441 tcggccgggc cgtacaggcg cccggattcg gcggacacga agctgaccac gccgaacggc
     4501 tgccccttga tcttcagcgg cacgccgatg taggaacgca agcccaggcc gcgcacgatc
     4561 tccaggtgtt cggcatcgct ggcggccgtt gccagcacat cgtcgctcag ttcccgcacc
     4621 agctccggag cgccggtgcg catgatgttc cagacgccga tgccggcatc gggtgcgggc
     4681 ggataccggc ggtgcagttc gtgcgccagc gcttccttgc gcgggtcgca gtgcgcaacc
     4741 gcaaggcggc gcagcgtgcc gtcgtcctcc agcacgtcga ccgcgcacca gtcagcgaag
     4801 gtcggcacgg ccagtttcgc cagtcggtcc agcgtggccg ggacgtccac cagatgcgca
     4861 agctccatgc tcgcctgcgc gagaaagtgc agatcctgct ccgcaatctt gcgcgcggtg
     4921 atgtccatgc acacgccggc catgcgcgcg ggttcgcctt gctcgtccag gtagatgcgt
     4981 ccccggcctt cgagccagcg caccgatccg tccggccata caatgcggta ttcgatacgg
     5041 tgatcgcggc gctcctgaag cgcgcgattc acggcgtcga taacggactg gcggtcggca
     5101 ggatggacca gctccccgta ggcctggacc gagtgggcgt aaccgtccgc cgagcggccg
     5161 tgcagcgtgg ccatgccgcc cagccactcg gacttgccgg tgcgcagatc tgcatgccat
     5221 gcgcccatcc ctccggcctc cagcgcgagc tgcaggcgct cctcggcctc gcgcagggcc
     5281 gcatgcgcat ggcttgcctc ggtcacgtca tgaccctgga tgaaaatgcc gatcacacgg
     5341 ccggcgctgt cggtgatcgg ctggtagacg aagtcgacgt atccttcgct ggccacgccg
     5401 gcatcgtcgc gcacgacgtg gcgcagcccg cgcccgacga agggcttgcc gctgcggtag
     5461 acctcgtcaa gcagatcgac gaaaccgaga ctcacgcttt cgggaagggt ttcgcgcaca
     5521 ggcttgccga tcacgtcgct gcgccgggca accttgagga agctggcatt ggccagctcg
     5581 aacacgtgat ccgggccgcg cacgaccgcc atgaagcccg ggcgcctgtt cgaacaggcg
     5641 cagcaggcgc tggttctctt cgacgcgctc gcgctcgagc aggactgagg cggtcgtctc
     5701 ggtgcagacg cagtagacgc cggcggtggc acccgattcg tcacgcgccg gcgataccga
     5761 gaacgtgaac cacgcatgct cctgcatgcc gtaacgcaac agcgtcagcg gctgattctc
     5821 gatatacacg gtttcgccgc caagcgcggt tcgaatgacg ggcccgatct cgcccttcac
     5881 ttcgggccag atatcccaga atgcccggcc cagcgcggcg gggtgccgcg cgccgagcag
     5941 ctcggcatag gcgtcgttgt agatcaggac aagctgcggc ccccaggcca catacatcgg
     6001 aaagtgcgcg tcgaggacga gccggaccac catgcgcagc ccgggggacc acccggacgg
     6061 aggtccgagc ggagtgctcg cccagtcacg ctcgcgcatc agcgca
//
LOCUS       DATLGF010000376         3569 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_94184,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000376 DATLGF010000000
VERSION     DATLGF010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94184"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="VM406_11580"
     CDS             <1..1311
                     /locus_tag="VM406_11580"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HVL76646.1"
                     /translation="TLTVLLVALLAAWTTSSLGLSLAFGAFLAGMMLGETEFRHQVEA
                     TIRPFRDVLLGLFFIGVGMLFDPTAIPAIWHWAVAGTIALLSIKTVLVMLLVRRAGLE
                     DVTAWRTGLLLAVGGEFGFALLALALGANVIDARLGQIALTSVLFSMIAGPFLIRYNR
                     RLAKRLSRQPPAPVEEGKVPQLGPETAPRLREHVIICGYGRIGQSVGNFLEEEHIPFV
                     ALDLDPVRVREAHTAGEPVFYGDAGERDILELLGLEHARLVVISHEDVAAAKKVLQHL
                     RTLRPELPVMVRTRDETHVDELHAAGALEVVPETLEAGLMIASQALLLLDVPVSRVVR
                     RIQQQRTTRYRLMREFFRGDPLSEGLDERDGHRLRAIVIAAGSTSVGRRLDQLPLDGA
                     TITALVRAGERQPNPQRDTVLEANDVLVVFGPQASLERAERAMQ"
     gene            complement(1326..2282)
                     /locus_tag="VM406_11585"
     CDS             complement(1326..2282)
                     /locus_tag="VM406_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006410675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HVL76647.1"
                     /translation="MPLSVERTALEQGQVWLYLCTIIAGLAFGFIWPRAASVLDILLW
                     PTLAVLLYATFLQVPLLHLREAFRDHRFLLASLAGNFVLLPLLVWGLTQVVALDPAVR
                     LGVLLVLLMPCTDWFITFSQQGGGQVARALAITPVNLFLQLLLLPFYLWLMADREALD
                     VVARPEFLPALAVVVLPLLLAAMSERWIEVEPRRAVLRGRLAWWPVPLLALVLFLVAA
                     VQAGTVRDAGGLLATLVPLFLLYLCLALGAARLLARLSGVTPEAGRTIAFSFGTRNSF
                     LVLPLALALPAGWEAAALVIVAQSLVELFGMLVYLRIVPRLF"
     gene            complement(2416..>3569)
                     /gene="amt"
                     /locus_tag="VM406_11590"
     CDS             complement(2416..>3569)
                     /gene="amt"
                     /locus_tag="VM406_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HVL76648.1"
                     /translation="PGLALFYGGLARSKNMLSVLMQVFVVFGLVMLLWAIYGYSLAFA
                     GEGMFFGGFERLFLRGITPDMLSSALATIPEYVFVSFQGTFAAITCALIVGSFAERIK
                     FSAVLLFVALWFTFSYVPVAHMVWGGGLLAEDGALDFAGGTVVHINAGIAGLVGAYVI
                     GKRLGYGREAMTPHSLTLTMVGASLLWVGWFGFNAGSAGAADGVAGLAMINTILATAA
                     ATLAWIAGEAMLKGKGSMLGAASGAVGGLVAVTPAAGFIGPMGSIILGLIAGFLCLWG
                     VNGLKKMLGADDAFDVFGVHGVGGIVGAILTGVFASPALGGTEGADFAMGAQVWIQTK
                     SVLITIVWSAVVAFIAYKVVDLLVGLRVTEDEEREGLDIASHGESAYHS"
BASE COUNT          669 a         1221 c         1188 g          491 t
ORIGIN      
        1 acactgaccg tgctgctggt cgccctgctg gccgcgtgga cgaccagcag tctcggcctg
       61 tcgctcgcct tcggcgcctt cctggcaggc atgatgctcg gcgagaccga gttccgccac
      121 caggtcgaag cgaccatccg cccgtttcgc gacgtgctgc tcggcctgtt cttcatcggc
      181 gtcggcatgc tgttcgaccc gaccgcgatt ccggcgatct ggcactgggc ggtcgcggga
      241 acgattgcac tgctatccat caagaccgtc ctggtgatgc tgctggtgcg ccgcgccggt
      301 ctcgaggacg tcaccgcatg gcgcaccggg ctgctgctgg ccgttggggg cgagttcggc
      361 ttcgcgctgc ttgcgcttgc gctaggtgcg aacgtgatcg acgcccgcct cggccagatt
      421 gcgctgactt cggtgctgtt ctcgatgatc gccggcccgt tcctgatccg ctacaaccgg
      481 cgcctggcga aacggctctc gcgccagcca cccgcacccg tcgaggaggg aaaggtgccg
      541 caactgggac cggagaccgc gccccggctg cgcgagcacg taatcatctg cggctatgga
      601 cggatcggtc agagcgtcgg caatttcctc gaagaggagc acattccgtt cgtcgccctc
      661 gatctcgatc cggtgcgcgt gcgcgaggcg catacggcgg gcgagccggt gttctacggc
      721 gacgccggcg aacgcgacat cctcgaactg ctcggactgg agcacgcgcg gctggtcgtc
      781 atcagccacg aggacgtggc cgcggcaaaa aaggtgctgc aacacctgcg cacgctacga
      841 ccggagttgc cggtgatggt gcgcacgcgt gacgaaaccc atgtcgacga actgcacgca
      901 gcgggcgcgc tggaagtggt gccggagacg ctggaggccg gcctgatgat cgcctcccag
      961 gccttgctgc tgctcgacgt tccggtctcg cgggtggtgc gccggatcca gcagcagcgc
     1021 acgacgcgct accgcctgat gcgcgagttc ttccgcggcg acccgctttc cgaaggattg
     1081 gacgagcgcg atggacaccg tctgcgcgcc atcgtaatcg cggcgggaag cacatccgtg
     1141 ggacggcgcc tggatcagct gccgctggac ggcgcaacca tcacggcgct ggtgcgcgcc
     1201 ggcgagcgac aaccgaatcc gcaacgtgac acggtactcg aagccaacga cgtgctggtc
     1261 gtcttcggcc cccaggcttc gttggagcgc gccgagcggg ccatgcagtg accggccagc
     1321 gtttctcaga acaggcgggg gacgatgcga aggtagacga gcatgccgaa cagctcgacc
     1381 agcgattgcg cgacgatcac cagcgccgcc gcctcccatc ccgccggcag ggccagcgcg
     1441 agcggcagca cgaggaagga attgcgcgtg ccgaagctga acgcgatggt gcggccggcc
     1501 tcaggggtga cgcctgacag gcgtgcgagg agacgggcgg cgccaagcgc caggcagaga
     1561 tacaacagaa acagcggcac cagtgtcgcc agcaatccgc ccgcgtcgcg caccgtaccg
     1621 gcctgaaccg ccgccaccag gaacagcacc agcgcgagca gcggcaccgg ccaccaggcc
     1681 agccgcccgc gcagcaccgc gcggcgcggt tccacctcga tccagcgctc tgacatcgcc
     1741 gcgagcagga gcgggagcac gacgactgcg agcgccggca agaattccgg ccgcgcgacc
     1801 acatccagcg cttcgcggtc cgccatcagc cacagataga acggcagcag cagcaattgc
     1861 aggaacaggt tgaccggcgt gattgcgagc gcgcgtgcaa cctgtccgcc gccctgctgg
     1921 ctgaacgtga tgaaccagtc cgtgcacggc atcagcagca ccagcagcac gcccaggcgc
     1981 acggccggat ccagtgccac cacctgcgtg aggccccaga ccagaagcgg cagcaggacg
     2041 aagttgccgg cgaggctggc gagcaggaag cgatgatcgc gaaatgcctc gcgcaggtgc
     2101 agcaacggta cctgcaggaa agtcgcgtac agcaggaccg ccagcgtggg ccagagcagg
     2161 atgtcgagga cggacgcagc gcgcggccag atgaagccga acgcgagtcc ggcgatgatg
     2221 gtgcacaggt agagccacac ctggccttgc tccagtgcgg ttcgctcgac ggacaatggc
     2281 atgtatgggc gcaatcgcta cgcggaatgt tcggtcacgc gcaattgtac gtgtcgggcg
     2341 gcggcatgaa aaaaagcgcc cggcgcgatg cgcgggcgct ttccctgacc ctgcggctgt
     2401 ccgcaggcgg cgcaatcagg agtgataggc cgattcgccg tggctcgcga tatccaggcc
     2461 ctcgcgctcc tcgtcttcgg tcacgcgcag gccgaccagc aggtccacca ccttgtacgc
     2521 gatgaaggcg acgactgcgg accagacgat ggtgatcaac acgctcttgg tctggatcca
     2581 gacctgcgcg cccatcgcga agtcagcgcc ttcggtgcca cccagcgccg gcgatgcgaa
     2641 cacgccggtc aggatcgcgc cgacaatacc gcccacgccg tgcacgccga acacgtcgaa
     2701 cgcgtcgtcc gcacccagca tcttcttcag gccgttcacg ccccacaggc acaggaaacc
     2761 ggcaatcagc ccgaggatga tcgaacccat cgggccgatg aagccggcgg ccggcgtcac
     2821 cgcgaccagc ccgccgaccg cgcccgaggc cgcgccgagc atcgagccct tgcccttgag
     2881 catggcttcg ccggcgatcc aggccagggt ggccgcggcc gtggcgagga tggtgttgat
     2941 cattgccagg cctgcgacgc cgtccgcggc gccggcggaa ccggcgttga acccgaacca
     3001 gcccacccac agcagcgagg cgccgaccat cgtcagcgtc aggctgtgcg gcgtcatcgc
     3061 ctcgcggccg tagcccaggc gcttgccgat cacgtaggcg cccaccaggc cggcgatgcc
     3121 ggcgttgatg tgcacgaccg tgcctccggc gaagtcgagc gcaccgtctt cagccagcag
     3181 gccgccgccc cagaccatgt gcgcaaccgg cacatagctg aaggtgaacc acagcgcgac
     3241 gaacagcagc accgcggaga acttgatgcg ctcggcaaag gagccgacga tcagcgcgca
     3301 ggtaatcgct gcgaaggtgc cctggaacga gacgaatacg tactcgggaa tggtggcaag
     3361 cgcactcgac agcatgtcgg gcgtgatgcc gcgcaggaac agtcgctcga acccgccgaa
     3421 gaacatgcct tcgccggcga acgccaggct gtagccgtag atcgcccaca gcagcatcac
     3481 caggccgaac accacgaaca cctgcatcag cacagacagc atgttcttgg aacgcgccag
     3541 gccgccgtag aacagcgcaa ggccgggga
//
LOCUS       DATLGF010000377         3883 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_231148, whole genome shotgun sequence.
ACCESSION   DATLGF010000377 DATLGF010000000
VERSION     DATLGF010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231148"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VM406_11595"
     CDS             <1..218
                     /locus_tag="VM406_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL76649.1"
                     /translation="PALAKVRAPTLLIVGGLDAPVIALNQQALAKLNVEKELVIVPGA
                     THLFEEAGTLEQVLDLAAAWFVRHFLS"
     gene            complement(293..1216)
                     /gene="truB"
                     /locus_tag="VM406_11600"
     CDS             complement(293..1216)
                     /gene="truB"
                     /locus_tag="VM406_11600"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HVL76650.1"
                     /translation="MQNPNKRVRVPVHGVLLLDKSAGMSSNDALIKAKRLLNAQKAGH
                     TGTLDPFATGLLPLCFGEATKFSQDLLEADKTYETRVHLGITTTTGDTEGEVLGRCEV
                     QVDRARIEQTLNAFRGEIDQVPPMHSALKRDGKPLYEYARAGVTLERAARRVTIRTLE
                     LIGFEAPYLDLRVTCSKGTYIRVLGEDIGTALGCGAHLQTLRRIGVGRLDLSNALTLE
                     QFAQIDESERAARLAPVDALLSSFPAIHLSPELVTRFLHGQRLALGKAGAGGDLPAGR
                     VRVYPEGSTRLLGSALLHEYGVLAPERLIAT"
     gene            complement(1220..1606)
                     /gene="rbfA"
                     /locus_tag="VM406_11605"
     CDS             complement(1220..1606)
                     /gene="rbfA"
                     /locus_tag="VM406_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HVL76651.1"
                     /translation="MAKHSKSIPGRGVRVADQIQRDLAEIIAYELKDPRVGMITITEV
                     QVTPDYAHAKVYFTTLVDSEEAVKNTLAGLRAASGFIRGQLGRRLTIHTTPELHFVHD
                     ISPVRGMELSKLIDEANASRARDAED"
     gene            complement(1725..>3883)
                     /gene="infB"
                     /locus_tag="VM406_11610"
     CDS             complement(1725..>3883)
                     /gene="infB"
                     /locus_tag="VM406_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HVL76652.1"
                     /translation="HDLARRVHHRLDLGDFVAQIKAMMNAPRKVMKAPEPAQAPAAKA
                     AEGTLHKPADKKPGEKPGDKKPVGGDKKSIKSANVASTWQDDAKKRSALKTRGGAAPG
                     GRDGWRSGPRGRRGGHHADDRESNFQVPTEAIVRDVHVPETITVAELAHKMAVKASEV
                     IKHLMKLGQMVTINQVLDQETAMIVVEEMGHRAFAAKLDDPEAMLEETGAQVDAEQLP
                     RAPVVTVMGHVDHGKTSLLDYIRRAKVAAGEAGGITQHIGAYHVETPRGIITFLDTPG
                     HEAFTAMRARGAKATDIVILVVAADDGVMPQTKEAIAHAKAAGVPLVVAINKVDKQGA
                     NLDRVTQELIAEGVVPEAYGGDAPFVPVSAKTGQGIDDLLENVSLQAEVLELTAPVDT
                     HAKGLVIEAKLDKGRGPVATVLVQSGTLKRGDVVLAGSSYGRVRAMLDENGKNIAEAG
                     PSIPVEIQGLTEVPAAGEEVMVMADERKAREIALFRQGKFRDVKLAKQQAAKLENMFE
                     QMGEGEVKNLPLIIKADVQGSQEAIVHAMQKLSTDEVRVQIVHAAVGGISESDVNLAM
                     ASKAVIIGFNTRADTSARKLAESSGIDIRYYNIIYDAVDDVKAAMSGMLAPEKREQAL
                     GLVEIRQVFHVSKVGSIAGCYVLDGVVRRGASVRLLRNNVVIWTGELESLKRFKDDVK
                     EVKAGFECGLSLKNFNDIQEGDQLEVFEVQEVARTL"
BASE COUNT          593 a         1358 c         1189 g          743 t
ORIGIN      
        1 gccccgcact ggcgaaggta cgcgcgccga cattgctgat cgtcggcgga ctcgatgcgc
       61 cggtgattgc gctgaaccag caggcgcttg cgaagttgaa tgtcgagaag gagctggtga
      121 tcgtgcccgg cgcgactcat ctgttcgagg aggcgggcac gctggaacag gtgctcgacc
      181 tggccgccgc ctggttcgtg cgtcacttcc tgtcgtgacg cagcttcgac ccgccggcgt
      241 gaacagcagg cgtcacgggg catggacgcg caagcccgca ggcaccccgg tctcaggtgg
      301 cgatcagccg ttccggcgcg agcacgccgt actcgtgcag caacgcgctg cccaagagac
      361 gtgtagaacc ttccggatac acccgcaccc tgccggccgg caagtccccg ccggcgcccg
      421 ccttgccgag cgccagccgc tggccgtgca ggaagcgcgt gaccagctcc ggcgacagat
      481 gaatcgcggg aaacgaagac agcaacgcgt ccaccggcgc gagccgcgcg gcccgctcgc
      541 tttcatcgat ctgcgcgaac tgctccagcg tcagtgcatt cgacagatcg agccggccga
      601 ccccgatgcg gcgcagcgtc tgcagatgcg cgccgcagcc gagtgccgtg ccgatgtcct
      661 cgccgagcac ccggatatag gttcccttgc tgcaggtcac acgcagatcg agatatggcg
      721 cttcgaagcc gatcagttcc agcgtgcgga tcgtgacccg ccgtgcggcg cgttccagcg
      781 tcacgccggc gcgcgcatat tcgtatagcg gcttgccgtc gcgcttgagc gccgaatgca
      841 tcggcggcac ctggtcgatc tcgccccgaa aggcgttaag cgtttgctct atccgcgcgc
      901 ggtcgacctg cacctcacag cgccccagca cctcgccttc ggtatcgccg gtagtcgtcg
      961 tgatgcccag gtgaacgcgc gtctcatagg tcttgtcggc ctcgagcagg tcctgggaaa
     1021 acttggtcgc ctcgccgaaa cagagcggta acaggccggt ggcgaacgga tcaagcgtgc
     1081 cggtatgccc cgccttctgc gcgttgagca ggcgcttggc cttgatcagc gcatcgttgc
     1141 tcgacatgcc ggcgctcttg tcgagcagca gcacgccgtg caccggcacg cgcactctct
     1201 tgttcgggtt ctgcacgcgt cagtcttccg cgtcgcgcgc ccggctcgca ttcgcttcgt
     1261 cgatcagctt cgacagctcc atcccgcgca ccggcgagat gtcgtgcacg aagtgcagct
     1321 cgggcgtggt gtggatcgtc aggcgccggc ccagctggcc gcggatgaag ccggacgccg
     1381 cgcgcaggcc ggccagcgtg ttcttcaccg cctcctcgct gtcgaccagg gtcgtgaagt
     1441 acaccttcgc atgcgcgtaa tccggcgtca cctgcacttc ggtgatcgtg atcatgccca
     1501 cgcgcgggtc cttcagttca tacgcgatga tctccgccag atcgcgctgg atctggtcgg
     1561 cgacgcggac gccgcgtccc ggtatcgatt tgctgtgctt ggccatggga aaaattcaaa
     1621 ctcataaatg gatcacggcg aacacggcgc gcgtggtgat cgttctcgtc accatgcgcg
     1681 ctgtgtccgc cgtgttgaac ccgccgcaat ccggcgtgga acgcttacag ggtacgggcc
     1741 acttcctgca cttcgaagac ttccagctgg tcgccttcct ggatgtcgtt gaagttcttc
     1801 agcgacaggc cgcactcgaa gccggccttc acttccttca cgtcgtcctt gaaacgcttg
     1861 agcgactcga gctccccggt ccagatgacc acgttgttgc gcagcaggcg caccgaggca
     1921 ccgcgccgca ccacgccgtc gagcacgtag cagccggcaa tcgagccgac cttgctgaca
     1981 tggaagacct ggcggatctc gaccagaccc aatgcctgct cgcgcttctc cggtgccagc
     2041 atgcccgaca tcgccgcctt cacgtcatcc accgcatcgt agatgatgtt gtagtaccgg
     2101 atgtcgatgc cgctggattc ggccagcttg cgcgccgacg tgtcggcgcg ggtgttgaag
     2161 ccgatgatga ccgccttcga ggccatcgcg aggttgacgt cggattcgct gatgccgccg
     2221 acggccgcgt gaacgatctg gacacgcacc tcgtcggtgg acagcttctg cattgcatgc
     2281 acgatcgctt cctgcgagcc ctgcacgtcc gccttgatga tcaacggcag gttcttcacc
     2341 tcgccctcgc ccatctgctc gaacatgttc tccagcttgg ccgcctgttg cttggccagc
     2401 ttgacgtcgc ggaacttgcc ctggcggaac agcgcgattt cgcgcgcctt gcgctcgtcg
     2461 gccatcacca ttacttcctc gccggctgcc ggcacttcgg tcaggccctg gatctcgacc
     2521 ggaatcgacg gcccggcctc ggcaatgttc ttcccgtttt cgtcgagcat cgcgcgcaca
     2581 cggccatagg aagaaccggc cagcaccacg tcgccgcgct tcagggtgcc ggactgcacc
     2641 agcacggtcg cgacaggacc gcgccccttg tccagcttgg cttcaatcac caggcccttg
     2701 gcgtgcgtgt ccaccggcgc ggtcaactcc agcacttcgg cctgcagcga gacgttctcc
     2761 agcagatcgt cgatgccctg gcccgtcttg gctgataccg gcacgaacgg cgcgtcgccg
     2821 ccgtacgctt ccggcaccac gccttcggcg atcagttcct gggtcacgcg atcgaggttc
     2881 gccccctgct tgtcgacctt gttgatcgcc accacgaggg gtacgccggc cgccttcgca
     2941 tgcgcgatcg cttccttcgt ttgcggcatg acgccgtcgt cggccgccac caccaggatc
     3001 acgatatcgg tcgcctttgc gccgcgcgca cgcattgccg taaatgcttc gtggcccggc
     3061 gtatccagga aggtgatgat cccgcgcggc gtctccacgt gatacgcacc gatgtgctgg
     3121 gtaatcccgc cggcttcgcc cgcggcgacc ttggcccggc ggatgtagtc cagcagcgag
     3181 gtcttgccgt ggtcgacgtg gcccatcacg gtcacgaccg gcgcgcgcgg cagctgctcg
     3241 gcatcgactt gagcgccggt ctcctcgagc atcgcttccg gatcgtccag cttcgcggcg
     3301 aacgcacgat ggcccatttc ctcgacgacg atcatcgccg tctcctggtc cagcacctgg
     3361 ttgatcgtca ccatctgacc aagcttcatc aagtgcttga tcacttcgga ggccttcacc
     3421 gccatcttgt gcgccagctc ggccaccgta atcgtttcag gcacatgcac gtcgcgcacg
     3481 atcgcttcgg tgggcacctg gaagttgctc tcgcgatcgt ctgcatggtg tccgccgcga
     3541 cggccacgcg ggccgctgcg ccagccgtcg cgcccgccgg gcgcggcacc gccgcgcgtc
     3601 ttgagcgcgg aacgcttctt cgcatcgtcc tgccaggtcg aggccacatt ggccgacttg
     3661 atcgacttct tgtcgccgcc gaccggcttc ttgtcacccg gcttttcacc cggcttcttg
     3721 tcggccggct tgtgcagcgt gccttccgcg gccttcgcgg ccggcgcctg cgccggctcg
     3781 ggcgccttca tgaccttgcg cggcgcgttc atcatcgcct tgatctgggc gacgaagtcg
     3841 cccagatcaa ggcgatgatg aacgcgccgc gcaaggtcat gaa
//
LOCUS       DATLGF010000378         5729 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_94201,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000378 DATLGF010000000
VERSION     DATLGF010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5729
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94201"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1420)
                     /locus_tag="VM406_11615"
     CDS             complement(146..1420)
                     /locus_tag="VM406_11615"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HVL76653.1"
                     /translation="MHVCVLGAGVVGITSAYHLMKEGHRVTLVDANEPGSGASFGNGA
                     QLSYSYVAPLAEPSVWTKWPSYLFSSDSPLTLRLQADSAQWRWLAGFLAASTRERARR
                     TTVELLRLAFLSRDELARLRRAEPIDFLHRVAGKLVMFSDPAALASARAQVAFQARYG
                     CRQQVLDAAGCMEIEPALAQSSVRWAGGVHTADEEVGDCALFCQALFERMRADADFRF
                     VRGAVTGVHTRAQRLHAVQAGREEIGADAFVLALGAQSTAFAQMAGFRLPVYPLKGYS
                     ITVPLTADSAWAPSVSITDMSRKIVFARLGNRLRVAGRVELVGMDRSIPRRAIDELKA
                     AVAADFPQCGLLDDAALAPWTGFRPATPTGLPIIGASPVPNLYLNVGHGSLGWTLACG
                     SAAMLAAQMNGRECAIDPRPYRFASGATHVAA"
     gene            complement(1577..2494)
                     /locus_tag="VM406_11620"
     CDS             complement(1577..2494)
                     /locus_tag="VM406_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL76654.1"
                     /translation="MKIRHVEAIHAVMLTGSVSGAARLLNLTQPAVSRTLQHAELQLG
                     FPLFQRSRNRLVPTREALLLDAEIAKLFHQLENVNRLAATLRAGSPGQLRVLMVPALG
                     QTILPAALAEFRRTHAEVPVAVRMLNSREIAAAMALHEADVGYVFGPAQHPALSGELV
                     CESPLVCAAPAGMFPGRRHIVLEDLIDKPVVMQDQGDYMSGVLADARREAGVDAPLGI
                     TVQTLHAALALAENGIGAAIVDAFTASSSDPARVQVLRLDPELQIPIYALRLEQAEMS
                     VQLRVFAECFARAARAVIERGRGGVGAPA"
     gene            complement(2574..2972)
                     /locus_tag="VM406_11625"
     CDS             complement(2574..2972)
                     /locus_tag="VM406_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HVL76655.1"
                     /translation="MCEIFVKADPALYEARARSIRLHGVVTSIRLENLFWETLAEIAT
                     RDGMTTSQLIAKLYDELVEYRGRIDNFASFLRVCCLRYQSLMAAGAISQDKSLPLAGY
                     RVAAVAPPASPAMPMRLAPRTTGRSKAAPG"
     gene            complement(3020..3601)
                     /locus_tag="VM406_11630"
     CDS             complement(3020..3601)
                     /locus_tag="VM406_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HVL76656.1"
                     /translation="MAAKKILMIVGDFGEDYEIMVPFQALLAVGHQVHAVCPDKRAGD
                     FVATAIHDFEGHQTYSEKPGHRFTLTHDFDKIEESGYDALVIPGGRAPEYLRLNPRVL
                     EMVRHFAGANKPIAAICHGAQILAAARVIEGRSCSAYPACRAEVELAGARFAEIEVSA
                     ATTDGNLVTAPAWPAHPAWIAQFLKVLGTEIVL"
     gene            3746..4630
                     /gene="rarD"
                     /locus_tag="VM406_11635"
     CDS             3746..4630
                     /gene="rarD"
                     /locus_tag="VM406_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HVL76657.1"
                     /translation="MRTGMLYAAGAYASWGLFPLYFKSLQALQPLEVLLHRMLWSLVF
                     VLAVLACRRQWRWIAEVMRKPRVLAGFAVSAVLLSTNWFIYIWAVNNDRVVDASLGYF
                     INPLFSVLLGYLLLRERLRPVQWAAVGLAAAGVLWLTWQVGQLPWVALSLATTFGLYG
                     LMRKTASLGALEGLTLETLLLFPFAAVWLAWLTAQGATGFGDVGLSTQLLLAAAGPIT
                     AIPLLMFAAGARRIPLSLLGLLQYIGPTLQLLIGVWVYNEPFGGARLIGFALIWGALA
                     VYSVEGLWRSRVARRVPV"
     gene            4797..>5729
                     /locus_tag="VM406_11640"
     CDS             4797..>5729
                     /locus_tag="VM406_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVL76658.1"
                     /translation="MAQYVYTMNRVGKIVPPKRQILKDISLSFFPGAKIGVLGLNGSG
                     KSTLLKIMAGVDKEIEGEAIPMPGLNIGYLPQEPQLDPEMTVRQAVESGLGEIAEAQA
                     KLDAVYAAYAEPDADFDALAAEQARLEALIATSGGGNLDQQLEVAADALRLPPWDAKV
                     GVLSGGEKRRVALCRLLLSKPDMLLLDEPTNHLDAESVDWLEQFLQRFPGTVVAITHD
                     RYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEARLKQEEAQESARQKTIAKELEWV
                     RQNPKGRQAKSKARLARFNELSEHEYQKRNETQEI"
BASE COUNT          931 a         1909 c         1944 g          945 t
ORIGIN      
        1 gccgccacca tcagcgaggt ggccgccagc gccatcaggg tcttggaaat gcgcatggtt
       61 tctttctcct cttgtcgtta aggtacaact tgaccggtcg gatgaccggg gcaaaagcat
      121 cggccgggcg gcgtgccccg ccagactagg cggcgacgtg cgtcgcgccc gatgcaaatc
      181 tgtacggccg cggatcgatc gcacactcgc gcccgttcat ctgcgcggcc agcatcgccg
      241 cactgccgca ggcgagcgtc cagcccagac tgccatggcc gacgttcagg tacaggttgg
      301 gtacgggcga ggcgccgatg atcggcagcc cggtgggcgt agcggggcga aaaccggtcc
      361 agggcgcaag cgcggcatcg tcgagaaggc cgcactgcgg aaaatccgca gcgaccgcgg
      421 ccttgagctc gtcgatcgcg cgccgcggta tcgagcgatc catcccgacc agttcgacac
      481 gtccggccac gcgcagacgg tttcccagcc tggcgaacac gatcttgcgc gacatgtcgg
      541 tgatcgacac ggaaggcgcc caggcggaat ccgcggtgag cggaacggtg atcgaatagc
      601 ccttgagcgg gtagaccggc aggcggaatc cggccatctg cgcgaaggcc gtgctttgcg
      661 cacccagcgc gagcacgaac gcgtcggcac cgatctcctc gcggcctgcc tgcaccgcgt
      721 gcaggcgttg ggcgcgcgtg tgcacaccgg tgacggcgcc gcgcacgaag cggaaatccg
      781 cgtcggcgcg catgcgctcg aacagcgcct ggcagaacag cgcgcaatcg ccgacctctt
      841 catcggccgt atggacgccg ccggcccagc ggacggagga ctgcgccagc gcaggctcga
      901 tctccatgca gccggccgcg tcgagaacct gctggcggca gccgtagcgc gcctggaagg
      961 caacctgggc ccgtgccgac gccagcgcgg ccgggtcgga aaacatcacc agcttgccgg
     1021 cgacgcggtg caggaaatcg atcggctccg cgcggcgcag gcgcgcgagt tcgtcacggc
     1081 tcaggaacgc caggcgcagc agttccaccg tcgttcggcg cgcgcgctcg cgtgtgctgg
     1141 cggcaagaaa cccggccagc cagcgccatt gggcgctgtc ggcctgcagt cgcagcgtca
     1201 gcggggaatc gctcgaaaac agataggacg gccatttggt ccagacgctg ggctcggcca
     1261 gcggcgccac atacgaatag ctcagctggg cgccgttgcc gaagcttgcg cccgaacccg
     1321 gttcattcgc gtcgacgagc gtgacgcgat gcccttcctt catcagatgg taggcgctgg
     1381 tgatgccgac gacacccgcg cccagtacgc agacatgcat gctttacaaa ccttcgtgtt
     1441 gcgcgtcgtg caggccaagg gacctgttcg acgcgttcaa tggcgcgaag tttggttttg
     1501 cgttgtccgt gagtcaaatg ccgattcgat gccggccatt accgttcgtc ataccggagg
     1561 ggtatgcaag cgcgcttcag gctggtgcgc ccaccccgcc gcgaccgcgc tcaatcaccg
     1621 cgcgcgcggc gcgcgcgaag cactcggcaa agacgcgcag ctgcaccgac atttccgcct
     1681 gttcaagccg cagcgcgtag atcggaatct ggagctccgg atccaggcgc agcacctgca
     1741 cgcgcgcagg atcggacgag gacgcggtga aggcatcgac aatggccgcg cctatgccgt
     1801 tctcggccag tgcgagcgcg gcgtgcagcg tctgcacggt gatcccgagc ggcgcatcga
     1861 ccccggcttc gcggcgggca tcggcgagca cgccgctcat gtagtcgccc tggtcctgca
     1921 tgacgaccgg cttgtcgatc aggtcctcca gcacgatgtg gcgtctgccg gggaacatgc
     1981 cggccggcgc ggcgcagacc agcggactct cgcagaccag ttccccggag agcgccggat
     2041 gctgggccgg gccgaacaca tagccgacgt ccgcctcgtg cagcgccatc gccgcggcga
     2101 tctcgcgcga gttgagcatg cgcaccgcca ccgggacctc ggcgtgggtg cggcggaact
     2161 ccgccagcgc cgccggcagg atggtttgtc cgagcgcggg caccatcagc accctcaatt
     2221 gcccgggcga gccggcgcgc agggtggcgg ccagacggtt gacgttctcg agctgatgga
     2281 agagcttggc gatctccgcg tcgagcagca aggcttcccg ggtcggaacg aggcgattgc
     2341 gtgaacgctg gaacagcgga aaccccagct ggagttcggc gtgctggagg gtgcgggaga
     2401 cggcgggctg ggtcaggttg agcaggcgcg cggcgccgct gacggatccg gtcagcatga
     2461 ccgcgtggat ggcttctaca tgtcggatct tcattcgccc actctctccc ccgcgccgac
     2521 acttgcggca ggcgcgcggg aagggagtgt accgcgcctg gcgggcagcg ggttcagccg
     2581 ggcgcggcct tgctcctgcc cgtggtgcgc ggcgccagtc gcatcggcat cgcaggcgac
     2641 gccggcggcg cgactgcggc gacccggtac cccgccagcg gcaggctctt gtcctgcgaa
     2701 atcgccccgg cggccatcag cgactggtag cgcaggcagc acacgcgcag gaaggaagcg
     2761 aagttgtcga tgcggccgcg gtactcgacc agctcgtcgt acagcttggc aatcagctgg
     2821 ctggtcgtca tgccgtcgcg ggtggcgatt tccgcgagcg tttcccaaaa caggttttcc
     2881 aggcggatcg aggtgaccac gccgtgcagg cggatcgagc gggcgcgcgc ctcgtacagc
     2941 gcggggtcgg ccttgacgaa gatttcacac atgcgtgcct ccgttcagtc ggggccgcgg
     3001 cgcgcggccc cgcttgccgt tatagcacga tctcggtccc cagcaccttg aggaattggg
     3061 cgatccaggc aggatgcgcg ggccacgccg gagcggtcac caggttgccg tcggtggtcg
     3121 cggcgctgac ctcgatttcc gcaaagcgcg cgccggccag ttcgacctcg gcgcggcagg
     3181 ccggataggc cgagcagctg cgcccctcga tcaccctggc cgcggcgagg atctgcgcac
     3241 catggcagat tgccgcgatc ggcttgttcg cgccggcgaa gtgacgcacc atttccagca
     3301 cgcgcggatt caggcgcagg tattccggcg cgcggccgcc gggaatcacg agcgcgtcgt
     3361 agccgctttc ctcgatcttg tcgaagtcgt gcgtcaacgt gaatcggtgg cccggctttt
     3421 cgctgtaggt ctggtggccc tcgaaatcat ggatcgcggt ggcgacaaaa tcaccggcgc
     3481 gcttgtccgg gcagaccgcg tgcacctggt gcccgaccgc cagcagcgcc tggaacggca
     3541 ccatgatttc gtagtcctcg ccgaagtcgc cgacgatcat caggattttc ttcgcagcca
     3601 tggttgtctc ctttatgtga gtgaacgacc attgttcccc cgccccgccg cgcacgggta
     3661 atcgcccgtt actacatcgg cccttgcacg gcgcacatgc gggattgctg ggataatgag
     3721 ccgcttcccc actcccgcct ttcccatgcg aacaggaatg ctctatgccg ccggcgcgta
     3781 tgcgtcctgg gggctgtttc cgctgtactt caagtcgctg caagcactgc agccgcttga
     3841 agtcctgttg catcggatgc tgtggtcgct ggttttcgtg ctggccgtgc tggcctgccg
     3901 gcgtcagtgg cgctggatcg ccgaggtgat gcgcaagccg cgcgtgctgg ccggcttcgc
     3961 ggtctccgcg gtgctgcttt cgaccaactg gttcatctac atctgggcgg tcaacaacga
     4021 ccgcgtcgtt gacgccagcc tcggctactt catcaatccg ctattcagcg tgctgctcgg
     4081 ctacctgctg ctgcgcgagc ggctgcgccc ggtgcagtgg gcggcggtcg gcctggcggc
     4141 ggccggcgtg ctgtggctga cctggcaggt cggccagctc ccctgggttg cgctgtcgct
     4201 ggccaccacc ttcggcctgt acggattgat gcgcaagacc gcctcgctgg gcgcgctgga
     4261 aggcctgacg ctggagaccc tgctgctgtt cccgttcgcc gcggtctggc ttgcctggct
     4321 gaccgctcag ggcgcaaccg gcttcggcga cgtcggactg tccacccagc tgctgcttgc
     4381 cgcggcgggc ccgatcaccg cgattccgct gctgatgttc gccgccggcg cgcgccgcat
     4441 cccgctgtcg ctgctcggct tgttgcagta catcggcccg acgctgcagc tcctgatcgg
     4501 cgtgtgggtg tacaacgagc cgttcggcgg cgcgcgcctg atcggctttg cgctgatctg
     4561 gggagcgctg gctgtctatt cggtcgaggg cctgtggcga agccgggtcg cgcgccgggt
     4621 gccggtgtga aagagccggc cccgggtttc ctttcttgaa attcgagtga tccggctcaa
     4681 gcctgtgcga gcgcggcggg catgacttcg agcgacttac atgaagccgg cggtcgttgc
     4741 gccgcgcatc ggctatcctt gcggtctcga ttcaacgcac cggaaaccgc acctctatgg
     4801 ctcaatacgt ctacacaatg aatcgcgtgg gcaagatcgt tccgcccaag cgccagatcc
     4861 tgaaagacat ctcgctctcc ttcttccccg gcgccaagat cggcgtgctc ggcctgaatg
     4921 gctcgggcaa gtccacgctg ctcaagatca tggccggcgt ggacaaggag atcgagggcg
     4981 aggcgatccc gatgccgggc ctgaacatcg gctacctgcc gcaggaaccg cagctcgatc
     5041 cggaaatgac ggtacgccag gcagtggaat ccggcctcgg tgaaatcgcc gaggcccagg
     5101 ccaagctcga cgcggtctat gcggcctacg ccgagccgga tgccgacttc gacgcgctcg
     5161 ccgccgagca ggcgcgcctg gaagcgctga tcgccacctc cggcggcggc aacctcgacc
     5221 agcagctgga agtggccgcc gacgcgctgc gcctgccgcc gtgggacgcg aaggtcggcg
     5281 tgctgtcggg cggcgaaaag cgccgcgtcg cgctgtgccg cctgttgctg tcaaaaccgg
     5341 acatgctgct gctcgacgag ccgaccaacc acctcgacgc cgaatcggtc gactggctgg
     5401 agcagttcct gcagcgcttt ccgggcaccg tggtggcgat cacccacgac cgctacttcc
     5461 tcgacaacgc ggccgagtgg attctcgaac tggaccgcgg ccacggcatt ccctggaagg
     5521 gcaactacag ttcctggctg gaacagaagg aagcgcgcct gaagcaggaa gaagcgcagg
     5581 aatcggcgcg ccagaagacg atcgccaagg aactcgagtg ggtgcgccag aacccgaagg
     5641 gccgccaggc caagagcaag gcgcgcctgg cccgcttcaa cgagctgtcc gaacacgaat
     5701 accagaagcg caacgagacg caggaaatc
//
LOCUS       DATLGF010000379         7577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_213905, whole genome shotgun sequence.
ACCESSION   DATLGF010000379 DATLGF010000000
VERSION     DATLGF010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 7577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7577
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213905"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VM406_11645"
     CDS             <1..620
                     /locus_tag="VM406_11645"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVL76659.1"
                     /translation="WDVHYLGPDDQIIMTTANEIRIPAGVPVSVLLRTHDVIHSFSVP
                     NLHGKIDMIPGRRNHVRLQADHPGVFRGHCAEFCGAQHARMAFFVIAEPREQFEAWLA
                     AQRRPAAPPASELAARGRESFRRHCVECHTIRGVGEAERRGPDLTHLASRRTIGAGTL
                     ENNRANRLRWITQNELVKPGNAMPEFAHLEKQELEGLLEYLEGLR"
     gene            620..3136
                     /gene="ctaD"
                     /locus_tag="VM406_11650"
     CDS             620..3136
                     /gene="ctaD"
                     /locus_tag="VM406_11650"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HVL76660.1"
                     /translation="MATTPQTPSEVAWLHDRLYQTWANPPGIIGMLKVVNHTSIGLRF
                     LVTGFIFFLIGGVLAMLMRSQLAFPLNDLLDHKTYAEAFTMHGTTMMFFFAVPIMEGF
                     AVYLIPKMIGARDLIYPRLSSFGYWCYLFGGILIYSSFLVDAVPDGGWFMYVPLNSKE
                     FTPGSNADFWLLGVTFAEISAVAAGVELIVAILKSRTAGMAIHKMPIYCWAILVTAFM
                     IVFAFPPLILASILLEIERAFGLPFFDAAKGGDPLLWQHLFWIFGHPEVYIIFLPAAG
                     LVSTILPTFARRPLAAYTWVVMALIATGFISFGLWVHHMFAVGIPLLSLAFFSAASMA
                     VVIPTGIQVFSWIGTLWNGKPVLRVPLLYILGFLFIFVLGGLTGVMVALVPFDWQVHD
                     THFVVAHLHYVLFGGMVFPLFAAFYYWLPMFSGRMPSDRLSHLAFWMIFMGFNITFLP
                     MHVTGIMGMPRRSYTYLPEMGWEYLNLISTVGGFISAAGIGMLIVDFLLHFLHGKRCG
                     QNPWKAGTLDWAIAVPVPPYNFVALPVVTHREPLWADESVAERSLNGKGFLGHTNVHQ
                     RETLMSSALRGEPENVVVLPGNTITPFVAAVFTGLFFVGFVSQIYALSVVGAVLAVGA
                     LFVWAWQGGIRNECVAFDAGEGCKLNLHYANPRSPGWWGTLIALLADGTLFASLIFAY
                     FFLWTLSPQWPPTGYRDMSLLLPTAAMGMLLVASVLIEWGERSNRAGHTKRLQLGYLI
                     AAALGVAYIVLQVMAIGSTGFGAREHAYGALVYTITGFQFVHVGAAVLMSVFIALRVW
                     RGYISPLRPSEPAVVKLFWHYTVLQWLLGYGVIHVFPFLA"
     gene            complement(3678..6497)
                     /gene="treY"
                     /locus_tag="VM406_11655"
     CDS             complement(3678..6497)
                     /gene="treY"
                     /locus_tag="VM406_11655"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HVL76661.1"
                     /translation="MSMPRATARLQLHKDFDFDAAAAVVPYYAALGISHIYASPILTA
                     RSGSTHGYDIVDPTRINPELGGEDGLRRMVAAIRQAGLGLILDIVPNHMGVGPENPWW
                     QHVLEWGQNSPYARWFDIDWQPADETLHGKVMAPFLGQPYGDVLRAGEIRLCFDAMRG
                     KFYIGYFDHRFPLAPADYVTVLRTAASERLAGAISAFENFRGDPGNVSQHNAEMGFAR
                     LRELAATGGQTDIEGALAHFSSENAAGQDALHALLERQHYRLAWWRCAADGINWRRFF
                     EITDLVGVRVEQDDVFEATHALIFRLYSEGLIDGVRIDHVDGLADPGAYCRKLRARLQ
                     ALSVQRPVELPPAPAYIVVEKILAHGEPLRQDWAIDGTSGYDFMDQVGALLHDPAGEA
                     PLTLLWQEVTGSERDFLHEVRDARRQLLANNLSGEFEAATRALHMLARTDLRTRDFTL
                     GAIRRVFAELLVHFPVYRTYADSNGRDELDQEVIERALEAARSSVERTEVPLLDVLAR
                     WLGGDAPADSANPRVRYLHQRAITRFQQLTPPLAAKSVEDTAFYRYGRLLSRNEVGSD
                     PGQFSLTVSDFHVGCIGRAASFPRAMLTTATHDHKRGEDTRTRLTALSEMAQDWGRAV
                     RDWMQANARLRTELPADNGGPPAFAPQPADELMLYQMLLGAWPIGLAPQDREHMQAFA
                     ERIERWQTKALREAKQVSDWVLPNEAYEAGCRDFLHRILGTSGQGGGSSAFLEGMQEW
                     VRRTAAAGAAKSLVQTTLRLTVPGVPDLYQGADLWDFSMVDPDNRRPVDYPLRERLLA
                     DAPVLPAAALDDWESGRVKQHLIRTLLALRTRMPDLFTEGEYGPLVVEGEHAGRVIAF
                     ARRHREQMMVVVGAHLPVPLLARNRLLLDPAGFGGTEIVLPHSSGPVWHDALSGRKLD
                     VDGGRVPLAQIFTGMPAAVLLAAKE"
     gene            complement(6494..>7577)
                     /gene="malQ"
                     /locus_tag="VM406_11660"
     CDS             complement(6494..>7577)
                     /gene="malQ"
                     /locus_tag="VM406_11660"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HVL76662.1"
                     /translation="VQAFARDHAEEVEFHAFLQWQAERGLRLAQRAARQAGMPVGLVT
                     DLAVGADNGGSQAWSRQNEILTGLAVGAPPDAMNPRGQGWGLGAFSPHAMKAGGFHAY
                     IEMLRAAFAHAGGVRIDHVLGLHRLWLVPDGASPEEGAYLHYPRTDLLRLVALESWRH
                     RAIVIGEDLGTVPEGFHELLADAGLLGIRVLLFQRDGQRFLQPHEWPADAISTTTTHD
                     LPTIAGYWKARDIEWRARLDLLEVGQSEEEARDQRDNECAALWRALQQGGFAPHDAPQ
                     PGPDDAPVDEAIAFMAAAPAPLAMLPVEDLLGQVEQPNLPGTTDAHPNWRRRLPHPVD
                     SLLDDPDAARRLELINRTRTHGGEQQ"
BASE COUNT         1179 a         2567 c         2502 g         1329 t
ORIGIN      
        1 ggtgggacgt tcactacctg gggcccgacg accagatcat catgaccact gccaacgaaa
       61 tccgcattcc cgccggcgtg ccggtctcgg tgctgctgcg cacccacgac gtgatccaca
      121 gcttttcggt gccgaacctg cacggcaaga tcgacatgat tcccggccgg cgcaatcacg
      181 tgcgcctgca ggccgaccat ccgggcgtgt tccgcggcca ctgcgcggag ttctgcggcg
      241 cccagcatgc ccgcatggcg tttttcgtga tcgccgagcc aagggagcag ttcgaggcct
      301 ggctggcggc ccagcggcgc ccggcggccc cgcccgcgag cgaactggcc gcgcgcgggc
      361 gcgagtcgtt caggcggcat tgcgtcgaat gccacacgat acgcggcgtc ggcgaagcgg
      421 agcggcgcgg accggacctg acccatctgg ccagccggcg cacgataggc gcgggcacgc
      481 tggagaacaa ccgcgcgaac cggctgcgct ggatcaccca gaacgaactg gtcaagccgg
      541 gcaacgcgat gcctgagttc gcccacctcg aaaaacagga gctggaaggt ctgctcgaat
      601 acctggaagg cctgcgctga tggcgaccac gccccaaacc ccgagcgaag tcgcgtggct
      661 gcacgaccgc ctttaccaga cctgggccaa tccgcccggc atcatcggca tgctgaaggt
      721 ggtgaaccac accagcatcg gcctgcgctt cctggtcacc ggcttcatct tcttcctgat
      781 cggcggcgtg ctggcgatgc tgatgcgcag ccagcttgcg ttcccgctca acgacctgct
      841 cgatcacaag acctacgccg aagcgttcac gatgcacggc acgacgatga tgttcttctt
      901 cgccgtgccg atcatggaag gttttgcggt ctacctgatc ccgaagatga tcggagcgcg
      961 cgacctgatc tacccgcgcc tgtcctcgtt cggctactgg tgctatctgt tcggcggcat
     1021 cctgatctac tccagcttcc tggtcgacgc ggtgccggac ggcggctggt tcatgtacgt
     1081 gccgttgaac agcaaggaat tcacgcccgg cagcaatgcg gacttctggc tgctcggcgt
     1141 gacgttcgcc gagatttcgg cggtggcggc gggcgtcgag ctgatcgtcg cgatcctgaa
     1201 atcgcgcacg gccggcatgg cgatccacaa gatgccgatc tactgctggg cgatcctggt
     1261 cacggccttc atgatcgtgt tcgccttccc gccgctgatc ctggcctcga tcctgctcga
     1321 gatcgagcgc gcgttcggcc tgccgttctt cgatgccgcc aagggcggcg atccgctgct
     1381 gtggcagcac ctgttctgga tcttcggcca cccggaggtc tacatcatct tcctgccggc
     1441 tgccggactg gtctcgacca tcctccccac cttcgcgcgg cggccgctgg cggcctacac
     1501 ctgggtcgtg atggcgctga tcgccaccgg cttcatcagc ttcggcttgt gggtgcacca
     1561 catgttcgcg gtcggcatcc cgctgctgtc gctggccttc ttctcggccg cctcgatggc
     1621 ggtggtgatc ccgaccggca tccaggtgtt ctcgtggatc ggcacgctgt ggaacggcaa
     1681 gccggtgctg cgcgtgccgc tgctgtacat cctcggcttc ctgttcatct tcgtgcttgg
     1741 cggcctgacc ggcgtgatgg tcgcgctggt gccgttcgac tggcaggttc acgacaccca
     1801 cttcgtggtc gcccacctgc actacgtgct gttcggcggc atggtgtttc cgctgttcgc
     1861 cgcgttctac tactggctgc cgatgttctc cggccgcatg ccctcggacc gcctgtcgca
     1921 cctggcgttc tggatgatct tcatgggctt caacatcacc ttcctgccga tgcacgtcac
     1981 cggcatcatg ggcatgccgc ggcgcagcta cacctacctt cccgaaatgg gctgggaata
     2041 cctgaacctg atctccaccg tcggaggctt catctccgcc gccggcatcg gcatgctgat
     2101 cgtcgacttc ctgctgcatt tcctgcacgg caagcgctgc ggacagaacc catggaaggc
     2161 cggcacgctg gactgggcga tcgccgttcc ggttccgccg tacaacttcg tcgcgttgcc
     2221 ggtagtcacg caccgcgagc cgttgtgggc cgatgagagc gtcgccgagc gcagtcttaa
     2281 cggcaagggc ttcctcgggc ataccaacgt gcaccagcgc gaaacgctga tgagcagcgc
     2341 gctgcgcggt gagccggaaa acgtggtggt gctgcccggg aacacgatca ctcccttcgt
     2401 cgccgccgtc ttcaccggcc tgttcttcgt cggcttcgtc tcgcagatct acgcgctctc
     2461 ggtcgtcggc gccgtgctcg cggtcggcgc gctgttcgtc tgggcctggc agggcggcat
     2521 ccgcaacgag tgcgtggcct tcgacgccgg cgagggctgc aagttgaatc ttcactatgc
     2581 gaacccccgc tcgccgggat ggtggggcac gctgatcgcg ctcctggccg acggcacgct
     2641 gtttgcctcg ctgatcttcg cctacttttt cctctggacg ctgtcgcctc agtggccgcc
     2701 gaccggctat cgcgacatgt cgctgctgct gccaactgcc gcgatgggca tgctgctggt
     2761 cgccagtgtg ctgatcgagt ggggcgagcg cagcaaccgc gccgggcaca cgaagcggct
     2821 gcagctgggc tatctgatcg ccgccgcact cggtgtcgcg tacatcgtgc tgcaggtgat
     2881 ggcgatcggc tcgaccggct tcggcgcgcg cgagcatgca tacggcgcgc tggtttacac
     2941 gatcacgggc ttccagttcg tgcatgtggg ggcggcggtg ctgatgagcg tcttcatcgc
     3001 gctgcgcgtc tggcgcggct acatcagccc gctgcggccg agcgagccgg cggtggtgaa
     3061 gctgttctgg cactacacgg tgctgcagtg gctgctcgga tacggggtca tacacgtgtt
     3121 cccgttcctt gcctgagccg ggcgaaaggc gggtggatga gattactcgc tccgttcgga
     3181 acgacagagt tgggggcagt cgcctgaatg actgtcgtcc cgtacgcagt gagagacctc
     3241 atccacctgg caagcgagaa cgtacagcaa gtggatgaga ttcctcgctc cgctcggaac
     3301 gacagtgttg aggtccaccg ccaaaattac tgtcatcccg aacgaagtga gggacctcat
     3361 ccgcctggca agcgagaacg tacagcaagt ggatgagatt cctcgctccg ctcggaacga
     3421 cagtgttgag gtccaccgcc aaaattactg tcctcccgaa cgaagtgagg gacctcatcc
     3481 acctggcaag cgagaacgta cagcaagtgg atgagattcc tcgctccact cggaacgaca
     3541 gtgttgaggt caaccgccaa aatttctgtc atcccgaacg cagtgaggga cctcatccgc
     3601 ctggaaagcg agaaagtaca gcaagtggat gcgattcctc gctccgctcg ttacgacagg
     3661 cttgcgggcc atggcactca ttccttcgcc gcaagcagca ccgccgccgg catgccggtg
     3721 aagatctgcg caagcggcac gcggccgcca tccacgtcga gctttctgcc gctcagcgcg
     3781 tcgtgccaga ccgggccgct gctgtgcggc agcacgattt ccgtgccgcc gaacccggcc
     3841 gggtcgagca aaagccgatt gcgggccaac agcggcaccg gcaggtgcgc accgaccacg
     3901 accatcatct gctcgcggtg gcgccgcgcg aacgcgatca ctcttcccgc atgctctccc
     3961 tcgaccacca gcggaccgta ttcgccttcg gtgaacagat ccggcatgcg tgtgcgcagc
     4021 gcgagcagcg tgcggatcag atgctgcttg acgcgcccgg actcccagtc gtcgagcgcg
     4081 gccgcgggca gcacgggcgc atcggcgagc aggcgttcgc gcagcgggta atcgaccggg
     4141 cgccggttgt ccggatcgac catgctgaag tcccacaggt cggcgccctg gtagaggtcg
     4201 ggcacgccgg gcacagtcag ccgaagcgtg gtctgtacca ggctcttggc ggcgccggct
     4261 gcggccgtcc tgcgcaccca ttcctgcatt ccttcgagaa aggcggacga accgccgccc
     4321 tgcccgctgg tgccgaggat cctgtgcagg aaatcgcggc aaccggcctc gtaggcctcg
     4381 ttcggcaata cccagtcgct gacctgcttg gcctcgcgca gcgccttggt ctgccagcgc
     4441 tcgatgcgtt cggcgaacgc ctgcatgtgt tcacggtcct gcggcgcaag tccgatcggc
     4501 caggcgccga gcagcatctg gtagagcatc agttcatccg ccggctgcgg ggcgaaggcc
     4561 ggcggaccgc cgttgtccgc tggaagttcg gtgcgcagcc gggcattggc ttgcatccag
     4621 tcgcgcaccg cccgccccca gtcctgcgcc atctccgaca gcgcagtcag gcgggtgcgc
     4681 gtatcctcgc cgcgcttgtg gtcgtgcgtg gcggtggtca gcatcgcgcg cgggaacgag
     4741 gccgcgcggc cgatgcagcc gacatggaag tcggagacgg tgagcgaaaa ctggccgggg
     4801 tctgaaccca cttcattgcg cgagagcagg cgcccgtagc ggtagaacgc ggtgtcctcg
     4861 accgacttcg cggcaagcgg cggcgtcagc tgctgaaagc gcgtgatcgc gcgctggtgc
     4921 aggtagcgca cgcgcgggtt ggccgagtcg gccggcgcgt cgccgccgag ccagcgggcc
     4981 agcacgtcga gcagcggcac ttcggtgcgc tcgacgctgg agcgcgccgc ttcgagcgcg
     5041 cgttcgatca cctcctggtc gagttcgtcg cggccgttcg aatcggcata ggtgcggtag
     5101 accgggaagt gcacgagtag ctcggcaaat acgcgccgga tcgcacccag cgtgaaatcg
     5161 cgggtgcgca ggtcggtgcg cgcgagcatg tgcagcgcgc gcgtggcggc ctcgaactcg
     5221 ccggacaggt tgttggccag aagctgacgc cgcgcgtcgc gcacctcgtg cagaaagtcg
     5281 cgctcgctgc cggtcacctc ttgccacagc agcgtcagcg gcgcttcgcc ggccgggtca
     5341 tgcagcagcg cgccgacctg atccatgaag tcataaccgc tggtgccgtc gatcgcccag
     5401 tcctggcgca gcggctcgcc atgcgcaaga attttctcca ccacgatata ggcaggtgcc
     5461 ggcggcagct cgaccggacg ctgcaccgac agcgcctgca gccgcgcgcg cagcttgcgg
     5521 cagtaggcgc cggggtcggc caggccgtcg acatgatcga tgcgcacacc gtcgatcagg
     5581 ccttcgctgt agagccggaa gatcagtgca tgggtcgcct cgaacacgtc gtcctgctcg
     5641 acgcgcacgc cgaccaggtc ggtgatctcg aagaagcgtc gccagttgat gccatcggcc
     5701 gcgcagcgcc accacgcgag ccggtaatgc tggcgctcaa gcagcgcgtg cagcgcatcc
     5761 tgaccggccg cgttctcgga cgagaaatgc gcaagcgcgc cctcgatgtc ggtttgcccc
     5821 ccggtggcgg cgagctcgcg caggcgggca aatcccattt cggcattgtg ctggctgacg
     5881 ttgccggggt cgccgcggaa attctcgaac gccgagatcg cgcctgccag ccgctccgag
     5941 gcggcggtgc gcagcacggt cacatagtcc gccggcgcga gcggaaaacg atggtcgaaa
     6001 tagccgatgt agaacttgcc gcgcatcgcg tcgaagcaca gccggatctc gccggcgcgc
     6061 agcacgtcgc cgtagggctg gccgaggaaa ggcgccatca ccttgccgtg cagcgtttcg
     6121 tcggccggct gccagtcgat gtcgaaccag cgcgcatacg ggctgttctg tccccattcc
     6181 agcacgtgct gccaccacgg gttttccggg ccaacgccca tgtggttggg cacgatgtcg
     6241 aggatcaggc cgaggccggc ctggcggatc gcggcgacca tgcgacgcag cccgtcctcg
     6301 ccgcccagtt ccgggttgat gcgggtcggg tcgacgatgt cgtaaccgtg cgtggagccg
     6361 ctgcgcgcgg tcaggatcgg cgacgcatag atgtggctga tgccgagcgc cgcgtagtag
     6421 ggcacgaccg cggcggcggc atcgaaatcg aaatccttgt gcagctggag tcgcgcggtg
     6481 gcgcgaggca tgctcattgt tgttctcccc catgggtgcg cgtgcgattg atcagctcga
     6541 ggcggcgtgc ggcatccggg tcgtccagca ggctgtcgac cgggtgcggc aggcgccgcc
     6601 gccagttcgg atgggcgtcg gtcgtgcccg gcaggttcgg ctgctcaacc tggcccagca
     6661 ggtcttccac cggcagcatt gccagcggcg ccggcgcggc ggccatgaag gcgatcgcct
     6721 cgtccaccgg tgcgtcgtcc ggcccgggct gcggcgcgtc gtgcggcgcg aagccgccct
     6781 gctgcagcgc gcgccacagc gccgcgcatt cgttgtcgcg ctggtcgcgc gcctcctcct
     6841 cgctctgtcc gacctccagc agatccagcc tggcccgcca ttcgatgtcc ctggccttcc
     6901 agtagccggc aatggtcggc aagtcgtgcg tggtggtggt cgagatcgca tccgccggcc
     6961 actcgtgcgg ctgcaggaag cgctggccgt cgcgctggaa cagcagtacg cggatgccga
     7021 gcaggccggc gtcggccagc agttcgtgga agccctcggg caccgtgccg aggtcttccc
     7081 cgatcacgat ggcccggtgc cgccatgatt ccagcgccac caggcgcagc aggtcggtgc
     7141 gcgggtaatg caggtaggcc ccctcctccg gcgacgcacc gtccggcacc agccacagcc
     7201 ggtgcaggcc gagcacatgg tcgatgcgca cgccgccggc atgggcgaag gccgcgcgca
     7261 gcatctcgat gtacgcatgg aagccgccag ccttcatcgc atgcggcgag aacgcgccga
     7321 gtccccagcc ctgcccgcgc ggattcatcg catccggcgg cgcaccgacc gcgagcccgg
     7381 tcaggatctc gttctgcctg ctccaggcct ggctgccgcc gttgtccgca cccaccgcaa
     7441 gatccgtgac caggccgacc ggcatgccgg cctggcgcgc ggcccgctgc gccagccgca
     7501 gcccgcgttc ggcctgccat tgcaggaagg catgaaactc cacctcctcg gcatgatcgc
     7561 gggcaaaggc ctgcacc
//
LOCUS       DATLGF010000380         2752 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_61723,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000380 DATLGF010000000
VERSION     DATLGF010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61723"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VM406_11665"
     CDS             <1..764
                     /locus_tag="VM406_11665"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVL76663.1"
                     /translation="LVVGPFLVSLVVPRVSVFIFVVGLPYLAFAALAWQLGTTLEFFN
                     GLVFHTLAVVVALMVLLHVRFLRWREFLLERSLTEIAHHDPLTGLVNRGQLTVLGERE
                     LARAMRHRRPLAIALLDVDHFKHVNDTYGHDGGDLVLKALAQQCLSDVRAVDHVGRLG
                     GEEFVCILPDTAPEDAALCAERLRRNVEAMEIVTPGGVVHITISIGIANYREGCPGWE
                     ALIKEADMAMYAAKHQGRNRVVAAPLAPMPATEAA"
     gene            complement(765..2273)
                     /locus_tag="VM406_11670"
     CDS             complement(765..2273)
                     /locus_tag="VM406_11670"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HVL76664.1"
                     /translation="MLTPPYVAPARFNDPAAALDQVRAIYEANVTHLRDHLQRFVNGE
                     TLASRVRACYPYVRLQTSTVARADSRLSYGFVAGPGTYETTLTRPDLFGNYYFEQFRL
                     LLKNHGLTQEVQLEIGTSAQPIPAHFSLREHDHLEGSLSAERRLALRDLFDLPDLAAM
                     DDGIANGTFEAAPGHPHPLALFTAPRVDYSLHRLRHYTGTEAEHFQNFVLFTNYQFYI
                     DEFVRLGHEIMRSPPGSGSEEDDYIAFVEPGNVITRRVGQNVREGDDLGAPPPRLPQM
                     PAYHLLREDGSGITMVNIGVGPANAKTITDHVAVLRPHAWLMLGHCAGLRNSQQLGDY
                     VLAHGYVREDHVLDEDLPLWVPIPALAEIQLALEAAVAEVTRLTGYELKRIMRTGTVA
                     STDNRNWELLPHRTPERRFSQSRAVALDMESATIAANGFRFRVPYGTLLCVSDKPLHG
                     EIKLPGMANQFYRERVDQHLRIGIRAVELLRRQGADRLHSRKLRSFAEVAFQ"
     gene            complement(2462..>2752)
                     /locus_tag="VM406_11675"
     CDS             complement(2462..>2752)
                     /locus_tag="VM406_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVL76665.1"
                     /translation="KSGFSRLVPRADVVSASRLRKGERGIWQRRYGEHVIRDERDLRN
                     HLDYIHYNHVKHGYVKRALDWPHSSFHRYVARGVYPSDWGGSLEGLGSGAGE"
BASE COUNT          479 a          883 c          905 g          485 t
ORIGIN      
        1 gtctcgtcgt cggtccgttc ctggtctcgc ttgtcgtgcc gcgggtctcg gtgttcatct
       61 tcgttgtcgg cctgccttac ctcgcattcg ccgcgctggc atggcagctc ggcacgacac
      121 tcgaattttt caacggcctc gtcttccata cgcttgccgt cgtggtcgca ttgatggtgc
      181 tgctgcatgt gcgcttcctg cgctggcgcg aattcctgct cgaacgcagc cttaccgaga
      241 tcgcccacca cgacccgctg accgggctgg tcaaccgcgg ccagctgacc gtgctgggcg
      301 agcgcgaact ggcgcgcgcg atgcgccacc gccgcccgct tgcaatcgct ctgctcgacg
      361 tggaccactt caagcacgtc aatgacacct acgggcacga tggcggcgat ctcgtgctca
      421 aggcgctggc gcagcaatgc ctttccgacg tacgcgctgt cgaccatgtc ggccgcctcg
      481 gcggcgaaga attcgtctgc atcctgccgg atactgcacc ggaagacgcc gcgctttgcg
      541 ccgagcgcct gcgacggaac gtggaagcaa tggagatcgt gacaccgggg ggcgtcgtgc
      601 acatcacgat cagcatcggg attgcgaact atcgagaagg atgtcccggc tgggaagcgc
      661 tgatcaagga ggccgacatg gcgatgtacg cagccaagca ccagggccgc aaccgcgtcg
      721 tggcggcgcc gcttgccccg atgccggcga cggaagcagc gtagctactg gaacgccact
      781 tccgcaaaac tgcgcagctt gcggctgtgc agccggtccg cgccctgccg gcgcagcagc
      841 tccacggccc gtatgccgat gcgcaggtgc tgatcgacgc gctcgcgata gaactggttg
      901 gccatgcccg gcagcttgat ctcgccatgc aagggcttgt cggacacgca cagcagcgtg
      961 ccgtagggca cgcggaagcg gaagccgttc gccgcgatgg tggcgctctc catgtcgagc
     1021 gccaccgcgc ggctctggct gaagcggcgt tccggcgtgc ggtgcggcag cagttcccag
     1081 ttgcggttgt cggtgctggc caccgtcccg gtgcgcatga tgcgcttgag ttcgtagccg
     1141 gtgagccggg tcacttcggc cacggccgcc tccagtgcaa gctggatctc ggccagcgcc
     1201 gggatcggca cccacagcgg caggtcttcg tcgagcacat ggtcctcgcg cacatagccg
     1261 tgcgccagca cgtagtcgcc gagctgctgg gagttgcgca ggccggcgca gtggcccagc
     1321 atcagccagg catgaggacg cagcaccgcg acatggtcgg tgatggtctt tgcgttggcc
     1381 ggtcccacgc cgatgttgac catcgtgata ccgctgccgt cctcgcgcag caggtggtag
     1441 gccggcatct gcggcaggcg cggcggcggc gcgccgagat cgtcaccctc gcgcacgttc
     1501 tggccgacgc ggcgcgtgat cacattgccg ggttcgacga aggcgatgta atcgtcctct
     1561 tcggaacccg agcccggcgg actgcgcatg atctcgtggc cgagacgcac gaattcgtcg
     1621 atgtagaact ggtagttggt gaacagcacg aagttctgga agtgctccgc ctcggtccct
     1681 gtgtaatgac gcagccggtg cagagaataa tcgacccgcg gcgcggtaaa cagcgccagc
     1741 ggatgcggat ggccgggcgc ggcctcgaag gtgccattgg cgatgccgtc gtccatcgcg
     1801 gccaggtccg gcaggtcgaa cagatcgcgc agcgccaggc gccgctccgc gctcagactg
     1861 ccttccagat gatcgtgctc gcgcagcgag aaatgcgccg gaatcggctg ggcgctggtg
     1921 ccgatctcca gctggacttc ctgcgtcaag ccgtggttct tcagcagcaa tcggaattgc
     1981 tcgaagtaat aattgccgaa caggtcaggc cgggtcagcg tggtttcata ggtgccggga
     2041 ccggcaacga aaccgtagga caggcgcgag tcggcacggg cgaccgtact ggtctgcagg
     2101 cgcacgtacg gataacaggc tcgcacccgc gacgccaggg tttcgccgtt gacgaagcgt
     2161 tgcaggtggt cgcgcaggtg ggtgacgttg gcttcgtata tcgcacgaac ctgatcgagg
     2221 gcggcggcgg gatcattgaa gcgggcgggc gcaacatacg gcggcgttag caattggata
     2281 ctcctgtgac gcgggatggg tcgtcggcgt gcattagcat aacgtgatcc agcgctaaag
     2341 cgtttgcgac gtattagcct gtaggccgcg atgcagtccc agcaccaaac cccggttgga
     2401 atgcgcgcca agagctggga ttccatcccg gcctaccgtc atgacaaggc gaaccaacgg
     2461 ctcactcacc ggctcccgag ccgagcccct ccaagcttcc gccccagtcc gacggataaa
     2521 ccccgcgagc cacgtagcga tgaaacgaag aatgcggcca gtcgagagcg cgtttcacat
     2581 agccatgctt gacgtggttg taatggatgt aatcgagatg gttgcgcaga tcccgctcgt
     2641 cccggatcac atgctcccca taccgccgct gccagatacc gcgctcgccc ttgcgcaacc
     2701 ggctcgccga tacgacgtcg gcgcgaggga ccagccgcga aaagcccgac tt
//
LOCUS       DATLGF010000381         5621 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_120383, whole genome shotgun sequence.
ACCESSION   DATLGF010000381 DATLGF010000000
VERSION     DATLGF010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5621
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120383"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="VM406_11680"
     CDS             <1..1032
                     /locus_tag="VM406_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76666.1"
                     /translation="RLDILVPLALPPAELKADLLKQLRAPALATLAARARLAQTESFD
                     GFPHALPHEYWITRRAGLHAPADNSPAVAGAWMHAHDIAAEEGTWFALHPVHIHIARD
                     HLVLTDPQQLDLSDADSRTLFDIARPLFEEYGHALHYGNATTWFMRADQWQGLRTSTP
                     EAAAGHNVDLWLPRGPGERDWRRLQNEVQMHWFTHPLNEAREARGARPVNSLWLWGAA
                     QPAPPPRAPWQAAFNLGRWPYAAPGIPMSHATDASTLLAQRPEHVLLALDALIDPAIA
                     GDWGDWLAALEALERDWFAPLLEALRSGSIDEITLVPTGEGHLKEFVIRPSSLRKFWV
                     RPSLNPLFA"
     gene            1029..2723
                     /gene="recJ"
                     /locus_tag="VM406_11685"
     CDS             1029..2723
                     /gene="recJ"
                     /locus_tag="VM406_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HVL76667.1"
                     /translation="MTRISTRPYPFRTAELLRQQGVHPVLARLYAARGLADLRELQSE
                     LSALIAPAGLLHIDAAAIYLADAIGAGKKMTIVADYDCDGATACAVGLRGLRALGARV
                     DYIVPNRFEYGYGLTPEIVELTVRTTSPDIIVTVDNGIASIDGVAEAKRRGIDVVVTD
                     HHLPGDRLPDARVIVNPNQPACGFPSKNLAGVGVMFYVLLALRAELRKRGAFDAQSQP
                     KLDALLDLVALGTVADVVKLDANNRILVAQGIKRMRAGRMQPGVAALFRAAGREARRA
                     SPFDLGFALGPRLNAAGRLADMSLGIECLTTDDEGRAWAIAQELDRINRERRDIEAEM
                     QDTALALLDDFNPQDKTTIAVYDEGWHQGVIGIVASRLKDKFYRPTITFAPGGEGLLK
                     GSGRSISGFHLRDALDLVSKHAPTLIQKFGGHAMAAGLTIRADGMEGFAAAFEAVGRA
                     QLTSAQLERILETDGMLEDAYFTPQFCELLDNQVWGQGFAPPVFCDEFRVLNQRILKE
                     KHLKLLLEKNGRRYDAIWFNHVDDLPERARVAYRLDANEYNGVTRVQLMVEHAEAA"
     gene            2812..3916
                     /gene="prfB"
                     /locus_tag="VM406_11690"
     CDS             join(2812..2892,2894..3916)
                     /gene="prfB"
                     /locus_tag="VM406_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079118.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HVL76668.1"
                     /translation="MEAERLNALSALLEDLSRRATDLRRYLDFDVKSEKLEQVNGELE
                     DPNVWNDPKRAQDLGREKKSLEAIVLTLTQIGNDVRDASDLFEMAREEGDEATLLAIE
                     GDAAGLEQQVEEMEFRRMFSNPLDANNCFIEIQAGAGGTEAQDWASMLLRQYLRYCER
                     KDFKTEILEISDGEVAGIKTASIKVEGEYAYGFLRTETGVHRLVRKSPFDSAGGRHTS
                     FASVFVYPEVDESIEIEINPADVRTDTYRASGAGGQHINKTDSAVRLTHEPTGIVVQC
                     QNDRSQHRNRAEAWAMLKSRLYELELRKRMSEQQKMEDAKTDIGWGHQIRSYVLDQSR
                     IKDLRTNHEVGNTKAVLDGDLDDFIAASLKQGV"
     gene            3946..5472
                     /gene="lysS"
                     /locus_tag="VM406_11695"
     CDS             3946..5472
                     /gene="lysS"
                     /locus_tag="VM406_11695"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HVL76669.1"
                     /translation="MSNQQEAAVPAQDENAIIAERRAKLAALREQGPAFPNDFRPTHK
                     AADLHAEYGELPNEELEARNVKVAVAGRMMLKRVMGKASFATLQDASGQKADGRIQLF
                     ISNDNVGEESHAKFKHYDLGDILGAEGTLFKTRTGELSVKVTALRLLTKSLRPLPDKF
                     HGLHDQETKYRQRYVDLIMSEQTRRVFKQRTAAMNSMRRFMAEHGFMEVETPMLHPIP
                     GGAAAKPFITHHNALDMEMFLRIAPELYLKRLVVGGFERVFEVNRNFRNEGVSPRHNP
                     EFTMMEFYAAYVDYKWLMDFTESVIRQAAIDAHGTAVLSYQGRALDLSKPFERLTIVG
                     AINKYAPQYTNEQLHDAAFLRSELKKFGVEPLANAGIGALQLALFEETAESQLWDPTY
                     IIDYPVEVSPLARASDTVPGITERFELFITGREIANGFSELNDPEDQAARFLAQVEAK
                     NAGDEEAMYFDADYIRALEYAMPPTGGCGIGIDRLMMLITDSPNIRDVILFPHLRRED
                     "
BASE COUNT         1072 a         1968 c         1727 g          854 t
ORIGIN      
        1 cgcctcgaca tcctcgtccc ccttgccctg ccgcccgccg aactgaaggc cgacctgcta
       61 aagcagctcc gggccccggc actcgcgacg ctggccgcgc gcgcccggct tgcgcaaacc
      121 gagtccttcg acggcttccc gcacgccctc ccgcacgagt actggatcac ccgccgcgcc
      181 gggttgcacg cgccggccga caacagtccc gcggttgccg gcgcatggat gcacgcccat
      241 gacattgccg cggaagaagg cacctggttc gcgctgcacc cggtgcacat ccacatcgcc
      301 cgcgaccatc tggtgctgac cgacccgcag cagctggacc tgtcggacgc ggactcgcgc
      361 acgctgttcg acatcgcgcg cccgctgttc gaggaatacg gccacgcgct gcattacggc
      421 aacgccacca cctggttcat gcgcgccgat cagtggcaag gactgcgtac ctcgacaccg
      481 gaggcggccg ccggccacaa cgtcgacctg tggctgccgc gcggtcccgg cgaacgcgac
      541 tggcgcaggc tgcagaacga agtgcagatg cactggttca cccatccgct caacgaggca
      601 cgcgaggcgc gaggcgcgcg accggtgaat tccctgtggc tgtggggcgc tgctcaaccc
      661 gccccgccgc cgcgtgcacc gtggcaggcc gcgttcaatc tcggccgctg gccatacgcc
      721 gcgccaggca tcccgatgtc gcacgcaacc gatgcatcga cgctgctcgc gcaacggccg
      781 gaacacgtgc tgctcgcgct cgatgcgctg atcgaccccg ccatcgccgg cgactggggc
      841 gactggctcg ccgcgctgga ggcgctggag cgcgactggt tcgcgccgct gctcgaggcg
      901 ctgcgcagcg gcagcatcga cgagatcacg ctcgtgccca ccggcgaggg ccatttgaag
      961 gaattcgtga tccgcccatc ctcgctgcgc aaattctggg tgcgccccag cctgaatcca
     1021 ctcttcgcat gacacgcatc agcacccgcc cctacccgtt ccgcaccgcc gaactgctgc
     1081 gccagcaagg cgtgcatccg gtgctcgcgc gcctgtatgc cgcgcgcggc ctcgccgacc
     1141 tgcgcgaact gcaaagcgaa ctgagcgcgc tgatcgcgcc ggccggactg ctgcacatcg
     1201 atgcggccgc catctatctt gcggatgcga tcggcgccgg caagaagatg accatcgtcg
     1261 ccgattacga ctgcgacggc gcgaccgcct gcgcggtcgg cctgcgcggg ctgcgcgcgc
     1321 tcggcgcgcg tgtcgactac atcgtcccga atcgcttcga atacggctat ggcctgacgc
     1381 cagagatcgt cgaactgaca gtgcgcacga cgtcgcccga catcatcgtc accgtcgaca
     1441 acggcatcgc cagcatcgac ggcgtggccg aagcgaagcg ccgcggcatc gacgtggtcg
     1501 tcaccgacca ccacctgccc ggcgaccgtc tgccggatgc gcgcgtgatc gtcaacccga
     1561 accagccggc ctgcggcttt ccgagcaaga acctggccgg cgtcggggtg atgttctacg
     1621 tgctgcttgc gctgcgcgcg gagctgcgca agcgcggcgc gttcgacgcg cagagccagc
     1681 ccaagctcga tgcgctgctc gacctggtcg cgctgggcac ggtggccgac gtcgtcaagc
     1741 tcgacgcgaa caaccgcatc ctggttgcgc aaggcatcaa gcgcatgcgc gccggccgca
     1801 tgcaaccggg cgtggccgcg ttgtttcgcg ccgccggccg cgaagcgcgg cgcgcgtcgc
     1861 cgttcgatct cggcttcgcg ctaggcccgc ggctcaacgc cgccgggcgg ctggccgaca
     1921 tgtcgctcgg catcgaatgc ctgaccaccg acgacgaagg ccgcgcctgg gcgatcgcgc
     1981 aggaactgga tcgcatcaac cgcgagcggc gcgatatcga agccgagatg caggacaccg
     2041 cactcgcgct gcttgacgat ttcaatccgc aggacaagac cacgatcgcc gtctacgacg
     2101 aaggctggca ccagggcgtg atcggcatcg tcgcctcgcg cctcaaagac aagttctacc
     2161 gccccacaat caccttcgcc cccggcggcg aaggcctgtt gaaaggctcc ggccgctcga
     2221 tttccggctt ccacctgcgc gacgcgctgg acctggtctc caagcacgcg ccgacgctga
     2281 tccagaaatt cggcggccac gcgatggcgg ccggcctgac catccgtgcc gacggcatgg
     2341 aaggcttcgc cgccgccttc gaagcggtcg gccgcgcgca gctgaccagc gcccagctcg
     2401 agcgcattct ggagaccgac ggcatgctgg aagacgccta cttcaccccg cagttctgtg
     2461 aactgctcga caaccaggtc tggggccagg gcttcgcccc gccggtgttc tgcgacgagt
     2521 tccgcgtgct caaccagcgc atcctgaagg aaaagcacct gaagttgctg ttggaaaaga
     2581 acggccgccg ctacgacgca atctggttca accacgtcga cgacctgccc gaacgcgcgc
     2641 gcgtcgcgta tcggctcgac gcgaacgagt acaacggggt cacgcgggtg cagctgatgg
     2701 tggaacacgc agaagcggcg tgaaccggcc gccggtacgc ctcgccgcat gaaggtcgcc
     2761 ggccctcccc tgcgctataa tgcccggttt gaccgcattg cccctaccgc catggaagcc
     2821 gaacgcctga acgccctctc cgccctgctc gaagacctgt cgcgccgcgc gaccgatcta
     2881 cggaggtatc tttgacttcg atgtgaagtc agagaaactc gagcaagtca acggcgaact
     2941 cgaagacccg aacgtctgga acgatccgaa gcgcgcccag gatctcggcc gtgaaaagaa
     3001 atcactggaa gccatcgttc tcacgctgac ccagatcggc aacgacgtgc gcgacgcgtc
     3061 ggacctgttc gagatggcgc gcgaggaagg cgacgaagcc acgctgctgg caatcgaagg
     3121 cgacgccgcc ggcctggaac agcaggtcga ggagatggag ttccggcgca tgttctccaa
     3181 cccgctggac gccaacaatt gcttcatcga aatccaggcg ggcgcgggcg gcaccgaagc
     3241 gcaggactgg gcgtcgatgc tgctgcgtca gtacctgcgc tactgcgagc gcaaggactt
     3301 caagaccgaa atcctggaaa tctccgatgg cgaagtggcg ggcatcaaga ccgcctccat
     3361 caaggtcgag ggcgaatacg cgtatggctt tctgcgcacc gaaaccggcg tacaccggct
     3421 ggtgcgcaag tcgccgttcg actccgccgg cggacgccac acctcgttcg ccagcgtatt
     3481 cgtctacccg gaagtggatg aatcgatcga gatcgaaatc aatcccgccg acgtgcgcac
     3541 cgacacctac cgcgcctctg gcgccggcgg ccagcacatc aacaagaccg actccgcggt
     3601 gcgcctgacc cacgaaccga ccggcatcgt cgtgcagtgt cagaacgacc gctcgcagca
     3661 ccgcaaccgc gccgaagcct gggcgatgct caaatcgcgc ctgtacgaac tggaactgcg
     3721 caagcgcatg agcgagcagc agaagatgga agacgccaag accgacatcg gctggggtca
     3781 ccagatccgc tcctacgtgc ttgaccaatc gcgcatcaag gacctgcgca cgaaccacga
     3841 agtcggcaac accaaggccg tgctcgacgg cgacctcgac gatttcattg cggcatcgct
     3901 gaagcaaggc gtttgatcgc ctatcgcccc atcccggaac atctcatgag caatcaacaa
     3961 gaagccgccg tcccggccca ggacgaaaac gcgatcatcg ccgagcggcg agccaagctc
     4021 gccgcgctgc gcgagcaggg cccggcattc ccgaacgatt tccgccccac ccacaaggcg
     4081 gcggacctgc acgccgagta cggcgagctg cccaacgagg agctcgaagc caggaacgtc
     4141 aaggtggccg tcgccggccg catgatgttg aagcgcgtga tgggcaaggc ctcgtttgca
     4201 accctgcagg atgcgtccgg ccaaaaggcc gacggtcgca tccagctctt catctccaac
     4261 gacaatgtcg gcgaagagtc gcacgcaaag ttcaagcact acgacctggg agatatcctc
     4321 ggagcggaag gcacgctgtt caagacgcgt accggcgaac tgtcggtcaa ggtgaccgcg
     4381 ctgcgcctgt tgaccaagtc gctgcgcccg ctgccggaca agttccacgg cctgcatgac
     4441 caggaaacca aataccgcca gcgctatgtc gacctcatca tgagcgagca gacgcgccgc
     4501 gtgttcaagc agcgcacggc agcgatgaac tcgatgcgcc gcttcatggc cgagcatggc
     4561 ttcatggaag tcgagacgcc gatgctgcat ccgatccccg gcggcgccgc ggccaagccg
     4621 ttcatcacgc accacaacgc gctggacatg gaaatgttcc tgcgcatcgc gccagagctc
     4681 tatctgaagc ggctggtcgt gggcggcttc gagcgcgtgt tcgaggtcaa ccgcaatttc
     4741 cgcaacgagg gagtgtcgcc gcgccacaat cccgagttca cgatgatgga gttctacgcc
     4801 gcctatgtgg actacaagtg gctgatggac ttcaccgaat cagtgatccg ccaggccgcg
     4861 atcgatgcgc acggcaccgc ggtgctgagc taccagggac gcgcgctcga cctgtccaag
     4921 ccgtttgagc gcttgaccat cgtcggtgca atcaacaagt acgcgccgca atacacgaac
     4981 gagcagctgc atgacgcagc cttcctgcgc agtgagctga agaaattcgg cgtcgagccg
     5041 ctggccaacg ccggcatcgg cgcactgcag ctcgcgctgt tcgaggaaac tgccgaatcg
     5101 cagctgtggg atccgaccta catcatcgac tacccggtcg aggtctcccc gctggcgcgg
     5161 gcgtcggata cggtgcccgg catcaccgag cgcttcgagc tcttcatcac cggccgcgag
     5221 atcgccaatg gtttctccga gctcaatgat cccgaggatc aggccgcgcg ctttcttgcc
     5281 caggtcgagg cgaagaacgc cggcgacgag gaagcaatgt acttcgacgc cgactacatc
     5341 cgcgcgctcg aatacgcgat gcctccgacc ggcggctgcg gcatcggcat cgaccgcctg
     5401 atgatgctga tcaccgattc gccgaacatc cgcgacgtga tcctgttccc gcatctgcgc
     5461 cgggaagact gagtcgcagg cggaggatct catccgcgcc aatccggcac tgcagcagat
     5521 ctaccggatg aggtctttcg ctgccgctca ggacgacaag ccttccccca ctcctgggtc
     5581 atcctgagcc gcaggcggaa ggatctcatc cacgccaagc c
//
LOCUS       DATLGF010000382         2599 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_231336, whole genome shotgun sequence.
ACCESSION   DATLGF010000382 DATLGF010000000
VERSION     DATLGF010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231336"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VM406_11700"
     CDS             complement(<1..809)
                     /locus_tag="VM406_11700"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HVL76670.1"
                     /translation="MKADLISAVRRTFTRGIAGACTVVAAGTLVAAAPVAAQNKPVNW
                     TIASFSEGSSWYVYSVNLGELLREALPAGSTVDTPPIAGGLGNPMLVSSNKAQIAFGM
                     AVVGSWAYEGQSGFKQPLTNLRALVGGWDDYFLVPMARGTGFPNDLSGFFKEVRPKAR
                     VTLLTRGSVGAFGGQQLLEIAGAGESALAKTGGSYEYGSFDMVKTRFAGGSGDVFVQV
                     ATAGHPGITEIAQTTKVTFLQPSKQVLEGMTSRYGWGTNVMPKGTFPGQDT"
     gene            963..1400
                     /locus_tag="VM406_11705"
     CDS             963..1400
                     /locus_tag="VM406_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76671.1"
                     /translation="MILFINNRDALPASLLLLASHVQARSVLDALLGIAFSAVRGYLM
                     LGDASSLPWLVGPMGATAMLLFALPASPLGNVLSALIGVGCRQWLGEPLVARLRRFGA
                     GTVDDVRAALPASSGRRHHGGRGSTRHACSPVRRETIRTRLKW"
     gene            complement(1684..1998)
                     /locus_tag="VM406_11710"
     CDS             complement(1684..1998)
                     /locus_tag="VM406_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HVL76672.1"
                     /translation="MQCPVCKTVNLTMSERQGIEIDYCPQCRGVWLDRGELDKIIERS
                     QPSAQPVPPVQPQGQAAPPPQPGYDPRHDPRHDPRYDPRHGGYYKKKKREGFLSDLFD
                     FD"
     gene            complement(2029..>2599)
                     /locus_tag="VM406_11715"
     CDS             complement(2029..>2599)
                     /locus_tag="VM406_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HVL76673.1"
                     /translation="CIYIRNHYRETKQAIQSVDQVFAHQPFGPETGHIQPDPDAQTAV
                     FIVGSSRGGGLHALLWVQRMFPGHFKNFIFINARTVDAHVYGGEGALEKMRSEAQDTL
                     DFFVNFCQSHGMPSEAYLGFGTDAVSEVTGLCEDISRKYPNSIFFTSKLVFEHDNWFT
                     RLLHNQAALAIQRRLHFEGLQMVILPMKV"
BASE COUNT          439 a          842 c          826 g          492 t
ORIGIN      
        1 tccgtgtcct gaccggggaa ggtccccttc ggcatcacgt tggtgcccca gccatagcgg
       61 ctcgtcatcc cttcgagcac ctgcttggat ggctgcagga aggtcacctt ggtggtctgg
      121 gcgatttcgg taatgcccgg atggcctgcc gtggcgacct gcacgaacac atcgcccgat
      181 ccgcccgcaa agcgggtctt gaccatgtcg aacgagccgt actcgtagct gccgccggtc
      241 ttggccaatg cgctttcgcc ggccccggcg atctccagca gctgctggcc gccgaaggcg
      301 ccgacgctgc cgcgcgtgag cagggtcacg cgcgccttcg gccgcacttc cttgaagaac
      361 ccggaaagat cgttcgggaa gccggtgccg cgcgccatcg gcacgaggaa gtagtcgtcc
      421 cagcccccga ccagcgcccg cagattcgtc agcggctgct tgaagccgga ttggccttcg
      481 taggcccagc tcccgacgac ggccatgccg aaagcgatct gcgccttgtt cgaggatacg
      541 agcatcggat tgccgagacc gcccgcgatc ggcggcgtat cgacggtgga gccggccggc
      601 agcgcctcgc gcagcagttc gccgaggttc accgaataca cgtaccagct cgaaccctcg
      661 gaaaacgatg cgatggtcca gttcaccggc ttgttctgtg ccgcgacggg cgccgccgcg
      721 accagggttc cggccgccac cacagtgcag gcgccggcga tgccgcgggt gaatgtccgc
      781 cggacggcgg atatcagatc tgctttcatt acatctcctc gttatagtcg tcgttgccag
      841 atgcgcaggc cctggagcga ccgctcggat cgcccttcgg aaaagccctg gcaacagggg
      901 aaaacgtgtt cttgtcagtc caagtatagg aaggtccttc tcatcatatc aattaatatt
      961 ttgtgatcct ttttatcaat aaccgtgatg ccttgccggc gtccctcctg ctcttggcga
     1021 gccatgtgca ggcgcgctcc gttctcgatg ccctgctcgg catcgcgttt tccgctgtgc
     1081 gtggctatct catgctgggc gacgcttcgt ccctgccttg gctggtgggg ccaatgggcg
     1141 cgaccgcaat gctgctgttt gcattgccgg ccagcccgct cggcaatgtg ctttcggcgc
     1201 tcatcggcgt cggatgccgc cagtggctgg gcgagccctt ggtcgcccgc ctgcgtcgct
     1261 tcggcgctgg cactgtcgat gatgttcgcg ctgcgctgcc tgcatcctcc gggcgacgcc
     1321 accatggcgg tcgcggcagc actcggcacg cctgctcacc cgtacgccgg gagactattc
     1381 ggacaaggct gaagtggtag gccagctcat ggggcggcgg gctaggacga tgcgcacttc
     1441 agcgccgctt gccgaggggg tgccgctgtt cgcagagtcg ggtctgtctt gcgtgccggc
     1501 cgtccacggg aacgaacggc tggtcggagt gctgacgcag gccgagctga cgcccgcgtt
     1561 gtatcaaggc gccacggatg agcgccgaca gcgctcggtc gcatagctgg gcagccgcag
     1621 gatccaaacg acgacgaccg ggacgaagca agccagccgc aaccgccttt gaatccaatc
     1681 cgctcagtcg aaatcgaaca gatcggaaag aaagccttcg cgcttctttt tcttgtagta
     1741 accgccgtgg cgcgggtcgt agcgcggatc atggcgcgga tcatgccgcg gatcgtagcc
     1801 gggctgcggc gggggagccg cctgcccctg cggctgcacg ggcggaacgg gctgtgcgga
     1861 cggctgcgac cgctcgatga tcttgtccag ctcgccgcgg tcaagccata cgccccggca
     1921 ctgcgggcaa taatcgatct cgatcccctg tctttccgac atcgtcaggt tgacggtctt
     1981 gcataccgga cactgcatac gccctcctcg ctcaagagaa acggaaactc acaccttcat
     2041 cggcagtatg accatctgca ggccctcgaa gtgcaaccgc cgctggatcg cgagcgcggc
     2101 ctggttgtgc agcaggcggg tgaaccagtt gtcgtgctcg aacaccagct tgctggtgaa
     2161 gaagatcgag ttcggatact tgcggctgat gtcctcgcac aagccggtca cctcgctgac
     2221 cgcgtcggtt ccgaagccga gataggcctc cgacggcatg ccatggctct ggcagaagtt
     2281 cacgaagaag tccagcgtgt cctgcgcctc gctgcgcatc ttctcgagcg cgccttcgcc
     2341 gccgtagaca tgcgcgtcca cagtgcgcgc attgatgaag atgaagttct tgaagtggcc
     2401 ggggaacatg cgctgcaccc acagcagcgc atgcaacccg ccgccgcgcg aagagccgac
     2461 gatgaacacc gcggtctgcg cgtccggatc gggctggata tggccggtct ccgggccgaa
     2521 cggctggtgc gcaaacacct ggtcgaccga ctggatcgcc tgcttggtct cgcgatagtg
     2581 attgcggata tagatgcac
//
LOCUS       DATLGF010000383         4197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_94206,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000383 DATLGF010000000
VERSION     DATLGF010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94206"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            482..1381
                     /locus_tag="VM406_11720"
     CDS             482..1381
                     /locus_tag="VM406_11720"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVL76674.1"
                     /translation="MDSQRIPRIAFLGTGLMGMPMAKRLLAAGYPISAWNRTRAKAEA
                     VGASTTDTSAQAVAGADVVITMLQDGTAVRSVIGDALPALQRGAVVVDMSSTAHAEAL
                     ELHGLLDAREIGFMDAPVSGGVLGAEQGTLAIMVGGSPENFARVEAVLNCLGRPTLVG
                     PPGSGQIAKLCNQLIVGGTLALVAEALLLAQAGGADPAAVRAAIRGGFAESRILEVHG
                     ARMLARNFMPGGQVKSQAKDMENVLAAAHDTGLQLPLAEIVAGQFCDLLPHFPAADQA
                     AALLALERINPGWRLGTTEDRLP"
     gene            1486..2301
                     /locus_tag="VM406_11725"
     CDS             1486..2301
                     /locus_tag="VM406_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HVL76675.1"
                     /translation="MHRFCVQILLAALLAGCASRAPAPVASERRGTASSELSQFSGSH
                     AGDLPPGWAPIAILRDKQPTQYRLVQDSQDVVLHASAHRASSGLMYELAADPVQRPWL
                     QWRWKIKGPQLLGKGSPAERGSPARIVLGFDGDKDSLPFTEQILFETARVVTGHEFPY
                     ATLMYVWSEDLPPASIVQSRFSGRIRMIVVDSGADGLDQWRGFSRNIAEDYHRAFGEP
                     PGRLIGVGVLTDDEHTPGGTEAWYGDIRLTEAPTPASAAAETKPQSLFLTQSE"
     gene            complement(2278..3297)
                     /locus_tag="VM406_11730"
     CDS             complement(2278..3297)
                     /locus_tag="VM406_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HVL76676.1"
                     /translation="MTAPVQGIPPARAASFARLALENVGREYPNKLDHVMNDASEVLA
                     PRALHPVFYGSYDWHSCVHMHWLLARVLHLYPDAPEAAAIAELFDAHFTEEKIAAEVA
                     YLAQPSRTTFERTYGWAWLLKLQTALRALPSVRQEYDRWQQRMQPLADAFVERYLNFL
                     PLARYPIRVGTHPNSAFGLYFALQYAESACDRPLADAVRAKATAWFINDEVYPARYEP
                     GSDDFLSGGLMEAVLMARVLGTGFHAWQARFFPTAVDLQTWLEPVEVSDRTDPKLAHL
                     DGLNLSRAWCWRTLQPHLAGPLREEAGRAAQRHLAAALPHADTGDYAGTHWLASFALL
                     ALSEE"
     gene            complement(3294..>4197)
                     /locus_tag="VM406_11735"
     CDS             complement(3294..>4197)
                     /locus_tag="VM406_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008453019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HVL76677.1"
                     /translation="ACGHDGHVAMLLGAAFHLARSRNFDGTVYLIFQPAEEGGGGARR
                     MMDEGLFEQYPMDAVFGMHNWPGLPVGSFGISPGPMMASSNEFELVVRGKGAHAAQPH
                     KGVDPIMAAVQIAQSWQTIISRNRNPLESGVLSITQIHSGSATNIIPDEAVMVGTVRT
                     FGTEILDMIEARMRAIAHCTAQAFDAHAEFSFHRNYPPLINHAAETAFALEVMRELVG
                     ADHVNGQIEPSMGAEDFAFMLQERPGCYVFIGNGEGSHRDGGHGLGPCNLHNPSYDFN
                     DDLLPLGASFWVRLVERWFAEARA"
BASE COUNT          765 a         1275 c         1392 g          765 t
ORIGIN      
        1 ggcgtaaggt gttcatagga tgagcctttc ccatagaatt ggaccgtgtc atcaaccgga
       61 tctgcaatgc aggccaacgc aacggaaagt tgcgatggtc gcctgcgatt cgtagagttt
      121 gcatgtcttt gtcagggaaa acacgtggtg tgggtacggc tgcgacttcc tctcaaggca
      181 cgcagatcgc aggatcggaa aggcgtgtgg cgggagcgac gttggtcgga aatcgcctcg
      241 tttgagtcgc cgccggtcct tcccattccg caagtccgat ctgatcaatt gcggtagtca
      301 ggccgcggct tcccaagcga atcacaccgc gcgtggaggg ggcagcggaa tggatgtggc
      361 gcttctgtgt gtgagcgagt actcactatg cgagccgaaa acgccatgca aatcatgtct
      421 ggctgacatg atacggttct ggcatcgtac gccagtgtca gcaaggaaag ataggtagaa
      481 catggactct cagcggattc cccggattgc gtttttaggc accggattga tgggcatgcc
      541 gatggcgaaa agattgctgg cggccggcta cccgatttcg gcctggaacc gaacgcgcgc
      601 aaaggccgaa gcagtggggg cgtcgaccac agatacatcg gctcaggcag tggccggtgc
      661 ggacgtggtg attacgatgc tgcaggacgg tacggccgtg cgcagcgtca tcggggacgc
      721 gctgcccgca ttgcaacggg gggcggtggt ggtcgacatg agttcgaccg ctcatgccga
      781 ggcgctcgaa ctgcacggct tgctggacgc gcgtgaaata ggcttcatgg acgcgccggt
      841 ttctggcggc gtgctcggtg cggaacaggg cacgcttgcc atcatggtcg gcggcagccc
      901 cgagaacttt gcccgggtcg aggctgtgct gaattgcctc ggcaggccga ccttggtggg
      961 gccgcccgga agtggtcaga tcgccaaact atgcaaccag ctcatcgtcg gtggcacgct
     1021 cgcattggtc gccgaggcac tgctgctcgc gcaagcgggt ggagccgatc ctgccgccgt
     1081 gcgggcggcg attcggggcg gcttcgccga gagccgtatc ctcgaggtcc acggcgcgcg
     1141 tatgctcgcg cgcaacttca tgcccggtgg ccaggtgaaa agccaggcga aggatatgga
     1201 gaatgtgctc gcggctgcgc acgatacggg gctgcagctg ccgcttgccg agattgtcgc
     1261 tggccagttc tgcgatcttt tgccgcactt cccggcggca gaccaagccg cggccctgct
     1321 tgcgctcgag cgcatcaatc ccggatggcg tcttggaacc actgaagatc gcctcccttg
     1381 aatgcgtcaa tcgcgatcaa ggcgagccag gctgtacaat ttggccccaa gagttgccac
     1441 gcattcactt catcacacca ctctcggtag gacttgagcc accctatgca tcgcttctgc
     1501 gtccagatcc tgttggcggc acttctggcc ggctgcgcga gccgggctcc ggccccggtg
     1561 gcctcggagc ggcgaggaac ggcctcgtcc gaactctcgc aatttagcgg cagccacgcc
     1621 ggagacctgc cgcctggatg ggcgccgatt gcaattctgc gcgacaaaca acccacgcaa
     1681 taccggcttg tgcaggattc gcaggatgtc gtgctgcatg cgagcgcaca tcgggcctcg
     1741 tccgggctga tgtatgaact ggcagcagat ccggtgcagc ggccgtggct gcaatggcga
     1801 tggaagatca aggggccgca gcttctcgga aaagggtcgc cggccgaacg cggctcgccg
     1861 gcgcgcatcg tacttggctt cgacggcgac aaggactcgc tgccattcac ggagcagatt
     1921 ctgttcgaga cggcgcgggt ggtcacaggg catgagtttc cctatgcgac gcttatgtac
     1981 gtctggagcg aggaccttcc gcccgcgtcg atcgtgcaaa gccgcttcag cgggcgaatc
     2041 aggatgattg tggtcgacag cggcgccgat ggcctcgatc aatggcgcgg gttctcccgc
     2101 aatattgcgg aggactacca ccgcgccttt ggagaaccgc ccgggaggct aatcggcgtc
     2161 ggcgtgctga ctgacgacga acacacgccc ggcggtaccg aggcatggta cggagacatc
     2221 cgcctgaccg aggccccaac gccggcgagt gccgccgccg aaaccaagcc gcagtcttta
     2281 ttcctcactc aaagcgagta gcgcgaagct tgcgagccaa tgggtgccgg catagtcgcc
     2341 ggtatccgca tgtggcagtg ccgccgcgag atgacgctgc gccgcgcgcc ccgcctcttc
     2401 gcgcagcggt ccggccaagt gcggctgcag cgtacgccag caccacgctc gtgagagatt
     2461 caatccgtcg aggtgggcga gtttcgggtc ggtgcggtcg ctgacttcga ctggctccag
     2521 ccaggtctgc agatcgacgg cggtcgggaa aaatcgcgcc tgccaggcgt ggaagcccgt
     2581 tcccagcacc cgagccatca ggacagcctc catcaatcct cccgacagga aatcgtccga
     2641 gcccggctcg taccgggccg gatagacctc gtcgttgatg aaccacgccg tcgcttttgc
     2701 acggaccgcg tccgcaagcg gccggtcaca tgccgattcc gcgtactgga gcgcgaagta
     2761 cagtccgaac gcgctgttcg gatgggtgcc cacccggatc ggatagcgcg cgagcggcag
     2821 aaagttcaag taacgctcga cgaaggcatc ggcgagcggc tgcatgcgct gctgccagcg
     2881 gtcatactcc tgacggacgc tgggcagcgc gcgcaaggcc gtctgcagtt tcagcagcca
     2941 ggcccagcca taggtgcgct cgaaggtcgt gcgcgaaggt tgcgcgaggt aagccacttc
     3001 ggcggcgatc ttttcctctg tgaagtgcgc gtcgaagagc tccgcgatgg cggcagcctc
     3061 tggcgcgtct ggatagaggt gcagcaccct ggccagaagc cagtgcatgt gaacgcagga
     3121 gtgccagtcg tagctgccgt agaacacggg atgcagcgca cgtggagcaa ggacttcgct
     3181 cgcgtcgttc atcacgtggt ccagcttgtt cggatattcg cggccgacgt tctccagcgc
     3241 caggcgggcg aacgacgcgg cccgcgccgg gggaatgcct tgaacgggcg cggtcatgcc
     3301 cgcgcctctg cgaaccaacg ctcgaccagt ctcacccaaa agctcgcgcc caggggaagc
     3361 aggtcgtcgt tgaaatcgta gctcgggttg tgcagattgc acgggcccaa tccgtgtccg
     3421 ccgtcgcggt ggcttccctc tccattgccg atgaagacat aacagccggg tctttcctgg
     3481 agcataaagg cgaaatcctc ggcgcccatc gagggctcga tctgcccgtt cacatggtcc
     3541 gcgccgacca gctcgcgcat gacctcgagc gcgaatgcgg tctccgcagc atggttgatc
     3601 aacggagggt aattgcgatg gaagctgaac tcggcgtgcg cgtcgaacgc ctgcgccgtg
     3661 cagtgcgcga tcgcgcgcat gcgcgcctcg atcatgtcca ggatctcggt gccgaaggtg
     3721 cgcacggtac cgaccatgac tgcttcatca gggatgatgt tggttgcgct gcccgagtga
     3781 atctgtgtga tggagagtac gccggattcg agcggattcc ggttgcgcga gatgatggtc
     3841 tgccagctct gggcaatctg gacagctgcc atgatcggat caacgccttt gtgcggctgg
     3901 gccgcgtgcg cacctttgcc gcgcacgacc agttcgaatt cgttcgagga cgccatcatc
     3961 gggccgggcg agatgccgaa gctgcctacg gggagtcccg gccagttgtg catgccgaag
     4021 acggcgtcca tcggatactg ctcgaacagg ccctcgtcca tcatccggcg cgcaccgccg
     4081 ccgccttcct cggcgggctg gaagatcaga tagacggtgc cgtcgaaatt ccggctgcgg
     4141 gcgaggtgaa aggctgcgcc cagcagcatc gctacgtggc cgtcatgtcc gcatgcg
//
LOCUS       DATLGF010000384         5846 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_61985,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000384 DATLGF010000000
VERSION     DATLGF010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61985"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /gene="rpoN"
                     /locus_tag="VM406_11740"
     CDS             <1..695
                     /gene="rpoN"
                     /locus_tag="VM406_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HVL76678.1"
                     /translation="EDLREVQQVIKRCNPHPGAAFATDASDFVVPDVIVKKAKNGWQV
                     TLNHDVMPRLRVNALYANILKQNKGEGSLTSQLQEAKWLIKNMRQRFDTILRVAQAIV
                     DRQRNFFSHGEVAMRPLVLREIADTLGLHESTISRVTTQKYMLTPHGMFELKYFFGSH
                     VATEAGGEASSTAIRALIKQLIGAEDPKNPFSDSKIADMLGEQGMVVARRTVAKYREA
                     LKIPPVNLRKSL"
     gene            805..1152
                     /gene="raiA"
                     /locus_tag="VM406_11745"
     CDS             805..1152
                     /gene="raiA"
                     /locus_tag="VM406_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HVL76679.1"
                     /translation="MNLTISGHHLDLTPAIRQYVETKLERIRRHFDHVIDVAVILAVE
                     KLPEKEKRQKAEITLRVRGKDIHAQSIAEDLYAAIDTLIDKLDRQVLKYKTRVQDHQH
                     QALKRMPDASAAA"
     gene            1291..2382
                     /locus_tag="VM406_11750"
     CDS             1291..2382
                     /locus_tag="VM406_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HVL76680.1"
                     /translation="MSEFIRTEIRNRIGIITLDRPKALNSLSLEMVRAITTALLGWRE
                     DPGVRAVLIRSTSERAFCAGGDIRFFYEMGKATPREGSALLEDFFTEEYALNHLIHFY
                     PKPYIALMDGIVMGGGMGVAQGGEANRMRIVTERTKMAMPEVNIGLFPDVGGSYFLSR
                     APGETGTWLGLTGETVGAADALYTDLADVFVPASQLPALERMLLEFDGADIRAAICEF
                     AAPYGDQVAPASSRLARARAAIDRHFALDSVAAIAASLARDDDEFAQASLAAMNKRSP
                     LMMGVTLAQLRRGAMMGVADCLRMERTMVRRSFEHGEVLEGVRALVIDKDNMPTWCES
                     DPEKLSAEMINRFFEPAWPPHAHPLRDLD"
     gene            complement(2416..3558)
                     /locus_tag="VM406_11755"
     CDS             complement(2416..3558)
                     /locus_tag="VM406_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HVL76681.1"
                     /translation="MDERLAPFAARQEASRGRRHPEAPPGSRSEFQRDRDRIIHSSAF
                     RRLEYKTQVFVNHEGDLFRTRLTHSIEVAQIARSIARNLRLNEDLVEAISLAHDLGHT
                     PFGHAGQEALNDCMREHGGFEHNLQSLRVVDELEQHYGAFDGLNLMFETREGILKHCS
                     LANARGLGDIGRRFIERKQPSLEAQLANLADEIAYNNHDIDDGLRSGLLTETQLGEVD
                     LYARHRREVESAYPGIRGRRAIHETIRRMINALIVDVIRTSEARIAGAAPQSVDDVRN
                     APVLIAFSETMQAEAAVLKRFLRLNLYQHYQVNRMTSKARRIVRELYEAFMAEPRLLP
                     PDYQVERGQGTEGAQRQARKVADYIAGMTDRYAMREHRRLFRVDEI"
     gene            complement(3561..5187)
                     /gene="aroKB"
                     /locus_tag="VM406_11760"
                     /pseudo
     CDS             complement(3561..5187)
                     /gene="aroKB"
                     /locus_tag="VM406_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665988.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional shikimate kinase/3-dehydroquinate
                     synthase AroKB"
     gene            complement(5238..>5846)
                     /locus_tag="VM406_11765"
     CDS             complement(5238..>5846)
                     /locus_tag="VM406_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HVL76682.1"
                     /translation="GNAVNLPAAGIGANIPGTVAFTLFNAAATRFIGLELSALEADGV
                     GKIISSPRVVTADSQPALIEQGEEIPYQKATSSGATSIEFKKANLKLEVIPQITPDGH
                     VILDVDVTKDSRGDPLAVGSTLVAINTKHVKTNVRVENGGTVVLGGIYQQNENDTTTK
                     VPLFGDLPVVGNLFRTNSKVSNKTELLIFLTPRILTDSAFNR"
BASE COUNT         1074 a         1848 c         1794 g         1130 t
ORIGIN      
        1 acgaggacct gcgcgaagtc cagcaggtga tcaagcgctg caatccgcat ccgggcgcgg
       61 cattcgcgac cgacgcctcg gacttcgtcg ttcccgacgt catcgtcaag aaggcgaaga
      121 acggatggca ggtcaccctc aatcacgacg taatgccgcg cctgcgcgtc aacgctctct
      181 acgcgaatat tctcaagcag aacaagggag aggggtcgct cacttcgcag ttgcaggaag
      241 caaagtggct gatcaagaac atgcgccagc gcttcgacac gatcttgcgc gtggcgcagg
      301 cgatcgtcga ccgccagcgc aattttttct ctcacggcga ggtggcgatg cgtccccttg
      361 tcttgcgcga aatagctgat acactgggtc tacacgagag tacgatctcg cgtgtaacca
      421 cgcagaaata tatgctcacg ccgcatggaa tgttcgagct gaagtatttc ttcggcagcc
      481 atgttgcgac cgaagcaggt ggcgaagcat cctcgacggc gatccgggca ctcatcaaac
      541 aactgatagg agcggaagac cccaagaacc cattttctga cagcaagatc gcagatatgt
      601 tgggtgagca gggcatggtg gtcgcccgtc gcacggttgc gaagtaccgc gaagccctca
      661 aaatcccccc cgtcaatctg cgtaaatccc tgtagttttt ccggcagtag ttgttcatgt
      721 tgctgtgttc cggcgtgccc cgtttgcgga gccacgcggg gccacgttgc gaaccgccag
      781 cgacaccatt cataggagcg ctgtatgaat ctcacgatca gtggacatca cctcgacttg
      841 acgcccgcca tccgtcaata tgtggaaaca aagctggaac gcatcaggcg ccatttcgat
      901 catgtcattg atgtagcagt catcctcgca gtagaaaagc tccccgagaa ggaaaagcgg
      961 cagaaggcag aaatcacctt gcgggtgcgc ggaaaagaca tccacgcgca aagcatcgcg
     1021 gaagatctgt atgccgccat cgacaccctg atcgacaagc tcgatcgcca agtcctcaaa
     1081 tacaagaccc gtgtacagga tcatcagcac caggcgctga agcgcatgcc ggacgcatcg
     1141 gcggccgcct gatcttccag tcacactgaa acgaggggcg cggatcgcgc ccctttttca
     1201 tgcccggttt tccccattgt catcccgccg actgcgcggc ctggcccttc tggctagaat
     1261 agccgctccc gcatgccttt ccattacgcc atgagcgaat tcatccgcac cgagatccgc
     1321 aaccgtatcg gcatcattac ccttgaccgc cccaaggccc tgaactccct gtccctcgaa
     1381 atggtgcggg cgatcacgac cgccctgctc ggctggcgcg aggatcctgg cgtgcgtgcg
     1441 gtcctgatcc gcagcacgag cgagcgggcc ttctgcgccg gtggcgacat ccgcttcttc
     1501 tacgagatgg gcaaggcgac gccgcgtgaa ggcagcgcgc tgctggaaga cttcttcacc
     1561 gaggagtacg cgctcaacca cctgattcac ttctacccga agccctacat cgcactgatg
     1621 gacggcatcg tgatgggcgg cggcatgggt gtggcccagg gcggcgaggc gaatcgcatg
     1681 cgcatcgtga ccgagcgcac gaagatggcc atgccggaag tgaacatcgg cttgtttcct
     1741 gatgtcggcg gcagctactt cctgtcgcgg gcgccgggtg aaacgggtac ctggctggga
     1801 ttgaccgggg aaacggtcgg cgcggccgac gcgctttaca cggatctggc cgacgtgttc
     1861 gtccccgctt cgcagctgcc ggcgctggag cgcatgttgc tcgaattcga tggtgcggac
     1921 atacgggcag cgatctgcga gtttgccgca ccgtatgggg accaggtcgc ccccgcgtcg
     1981 agccgtcttg cgcgcgcacg tgccgcaatc gatcggcatt ttgccctcga ttcggtcgcc
     2041 gcgatcgccg cgtctctcgc ccgcgacgat gacgagttcg cgcaggccag tctggccgcg
     2101 atgaacaagc gctccccgtt gatgatgggc gtgaccctcg cgcagctgcg ccgcggggcc
     2161 atgatgggcg tcgccgactg cctgcgcatg gaacgcacga tggtgcgccg ctctttcgag
     2221 catggcgaag tcctggaagg cgtgcgcgcg ctggtcatcg acaaggacaa catgccgacg
     2281 tggtgcgaaa gcgacccgga aaagctcagc gccgagatga tcaatcgctt tttcgagccg
     2341 gcatggccgc cgcatgcgca cccgctgcgc gacctcgact gatccctgca aaccgccccc
     2401 cccttggggc ggtttctaga tttcgtcgac cctgaacagg cgccggtgct cgcgcatcgc
     2461 atagcgatcc gtcatgccgg cgatatagtc ggccaccttg cgtgcctgac gctgcgcgcc
     2521 ttccgtgccc tggccacgct cgacctgata atcaggcggc aacagacgag gctcggccat
     2581 gaaggcctcg tatagctcgc gcacaatgcg gcgcgccttg ctggtcatcc ggttgacctg
     2641 atagtgctga tacagattca gccgcaggaa tcgcttgagc accgccgcct ccgcctgcat
     2701 cgtttccgaa aaggcgatca gcaccggtgc gttcctgaca tcgtcaacgc tctgcggagc
     2761 ggcgcccgca atgcgcgctt ccgaggtgcg gatcacgtcg acgatcagcg cattgatcat
     2821 gcgacgtatc gtctcgtgaa tcgcgcgtcg accgcgaatg cccggataag ccgattcgac
     2881 ttcccggcgg tgccgcgcgt acagatcgac ctccccgagt tgggtttcag tcagaagtcc
     2941 cgagcgcaag ccatcgtcga tgtcgtgatt gttgtaggcg atctcgtcgg cgagattggc
     3001 tagctgggct tccagcgagg gttgcttgcg ttcgatgaag cgccggccga tgtcacccag
     3061 accccgcgca ttggcgagcg agcagtgctt gagtatgcct tcgcgcgtct cgaacatcag
     3121 gttcaagccg tcgaacgcgc catagtgctg ctccagttca tcgaccaccc gcaggctctg
     3181 caggttgtgc tcgaagccgc catgctcgcg catgcagtcg ttcagcgcct cctggcccgc
     3241 atgcccgaac ggcgtgtggc cgaggtcgtg ggcaagcgaa atcgcctcga ccaagtcttc
     3301 attcaggcgc aggttgcgcg cgatcgagcg ggcgatctgc gccacttcga tgctgtgcgt
     3361 gagacgggtg cggaacaggt cgccttcgtg attgacgaag acctgcgtct tgtattcaag
     3421 ccggcggaat gcgctcgagt ggatgatgcg gtcacggtcg cgctggaact cggaacggga
     3481 gccaggcggc gcctcaggat gacgacgccc gcgcgatgcc tcctgtcggg cggcaaatgg
     3541 cgcgagtcgc tcgtccatgt ctacctcgcc acgcaaactt cgagggtctg acgcaacagc
     3601 gcttccggaa ccggcgtgat gacagccgtg cccaagggct tcagaaggat gaacttgatt
     3661 tcgccaccct cgtttttctt gtccacctgc atcagctcca gccagcgcgc aatgccgagc
     3721 tccggtgcct tgatcggcag tcccgctgca cgcacgaggc gcgtgatgcg tgcggcggtc
     3781 gcggcatcga tataaccgag ccgctgcgac aaatcggccg ccatcaccat tccgcatccg
     3841 acggcctcac cgtgcagcca ttcgccatac ccgagtcccg cctcgatcgc atgtccgaag
     3901 gtatggccga agttgaggat cgcgcgcagc ccgccttcac gctcgtcctg gcgcacgacc
     3961 tccgccttga tctcgcagga acgccggatc gcatgcgcca gtgccgcagg gtcgcgcgcc
     4021 ttcagtgcgt cgatattggc ttcgatccat tcgaagaatg cggcgtcgat gatcgcgcca
     4081 tgcttgatga cttcggccag tccggccgca agctcccggt cgggcagcgt gtccagcgtg
     4141 gaggtatcgg cgatcaccgc cttcggctgg taaaacgcgc cgatcatgtt cttgccgagc
     4201 ggatgattga tgccggtttt cccaccgacc gaagaatcga cctgcgccag cagggtcgtc
     4261 ggaacctgca cgaagggaac gccgcgcata tagctcgccg ccgcgaagcc ggtgagatcg
     4321 ccgatgacgc cgccgccgag cgcgatcagg gtcgtcttgc ggtcgcactt ctccgacaga
     4381 aggacatcga acacgcgcat caagctggac cagttctttt cttcctcgcc gtcgggcagg
     4441 acgacggtgg tcacctgctt gccggctgcc gcgaggtggc ggcagacggt atcgagatac
     4501 agcggcgcaa cggtggtgtt ggtcaccacg gcggctcgct ttccctccac gctgcgggcc
     4561 agcaagtcgc tgtccgcaag gagggaacga ccaatcgaga tcggataact gcggtcgccg
     4621 agctggactt cgagggtggt tgcggacggg gatggattca tcggttcggc atcaggtcgg
     4681 gtttcccgcc tgcgccgaca gcacgcctgc cgcttcgagc tgggaaacga tggtttgcac
     4741 cagcaattgt acgttaggac gtccggtctc cacgacgaag tcggccacct cgagatagta
     4801 gggctcgcgc tcgcgcgtga ggcgttcaag cgtctggcgc ggatcgccct gttgcagcag
     4861 cggccggttc ttgtcgtggc ccgtgcgctg gagaatactg gccacgcttg cgcgcaggaa
     4921 aatgacggtg ccgcgcgttt tcaggtattc gcggttttcg ggcttcagaa tcgccccgcc
     4981 gccggttgcc agcacgatat cgggccgggc ggtgagatcg cggatgacgt cggattcgcg
     5041 ttggcggaag ctggcctcgc cctcgatctc gaagatcagc ggaatcgaga ccccggtgcg
     5101 ggcctcgatc tcatggtcgg aatcgatgaa ctgcttgttc agacggcgtg ccaatgcacg
     5161 cccgaccgtg gtcttcccgg cccccatgag gccgaccagg aagatgctgc tgcctgcacc
     5221 ggtcttctga ctcacgtcta tcggttgaat gcgctgtctg taaggatgcg cggcgtgagg
     5281 aaaatcagca gttcagtctt gttgctgacc ttggaattgg tgcggaacag attgccgacc
     5341 actggcaaat cgccgaacag cggcaccttg gtcgtcgtat cgttctcgtt ctgctggtag
     5401 ataccaccga gcacgacagt gccgccgttt tccacgcgca cgttcgtctt cacatgcttg
     5461 gtgttgatcg cgaccagcgt cgaaccgacc gccagcggat cgcctcggct gtccttggtc
     5521 acgtctacgt ccaggatgac atgaccatcc ggcgtgatct gcggaatcac ctcgagcttc
     5581 aggttcgcct tcttgaactc gatcgaggtt gcgccgctgc tggtcgcctt ctgatacggg
     5641 atttcctcac cctgctcgat cagcgccggc tgcgagtccg ccgtcacgac acgcgggctg
     5701 gagatgatct taccgacgcc gtccgcttcc agtgccgaca attccagtcc gatgaagcgg
     5761 gttgccgccg cattgaacag cgtaaatgcc accgtacccg ggatgttggc gccgatgccg
     5821 gccgccggca ggttcaccgc gttgcc
//
LOCUS       DATLGF010000385         6590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_94253,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000385 DATLGF010000000
VERSION     DATLGF010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6590
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94253"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VM406_11770"
     CDS             <1..671
                     /locus_tag="VM406_11770"
                     /inference="COORDINATES: protein motif:HMM:NF039714.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HVL76683.1"
                     /translation="RTQSRLPQPVSVSQVDEEFTIMRERAERVFEYALPHLATLALSL
                     TPQQIASIERKFASNNEKFRKETLRKSPAEQRQARYEKVMKQAEYWFGKFDAAQKERI
                     RAASDARPMDYEIWMQERLRRQRELIAFLKKVQAERPARHAVEGMLRAYMGGVLHNFT
                     YTENKPFFDASRHGMAEMTAQIINLATPAQKAHANRRLQSLIDDGHKLAGIRSGQQVS
                     LQER"
     gene            complement(690..1604)
                     /gene="ttcA"
                     /locus_tag="VM406_11775"
     CDS             complement(690..1604)
                     /gene="ttcA"
                     /locus_tag="VM406_11775"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HVL76684.1"
                     /translation="MDPVLKEQNDARRADKIAFENNKLSKRLCRLVGQAIGDFNMIED
                     GDKVMVCLSGGKDSYGLLDILLTLRERAPIHFDIVAVNLDQKQPNFPEHVLPEYLTKI
                     GVPFHIEHQDTYSIVKRLIPEGKTTCSLCSRLRRGILYRVADELGATKIALGHHRDDI
                     LETFFLNMFFGAKLKGMPPKLQSDDGRHVVIRPLAYVKEADMERWAQVKQFPIIPCDL
                     CGSQENLQRKQIKAMLREWEKKHPGRVENIFSALSTVVPSHLMDRELFGFADLKATGV
                     ASADGDIAFDEEPCAPPPNVIRFGGTMD"
     gene            complement(1607..1999)
                     /locus_tag="VM406_11780"
     CDS             complement(1607..1999)
                     /locus_tag="VM406_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HVL76685.1"
                     /translation="MLSALSHPSLIDCRRLFLRNYEVMINIGVHEFEKKGEQRVLVNV
                     DLFIPLAVSTPREDQLDEVVDYDFMRDTIARRMAQGHIHLQETLCDDVARAMLAHPKV
                     RAVRVSTEKPDVYPDCDAVGVEVIHIKA"
     gene            complement(2009..2776)
                     /locus_tag="VM406_11785"
     CDS             complement(2009..2776)
                     /locus_tag="VM406_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL76686.1"
                     /translation="MPDTASKSVLVTGGARRIGRHIALAFAHRGWNVAIHYRNSAAQA
                     QSLADEIAATGRRAVLLHADLEDEQQARDLATRAIAAFGRLDCVVNNASLFEPDDAQR
                     FSPALLERHMRANVTAPLLLAQALHAATPEGGQAVVVNLLDQKLYNLNPDYLSYTLSK
                     AALHTATTTLAQALAPKLRVVGVAPGITMVSGDQTEEGFAKAHAMTPLGRSSTPEDVA
                     AAVCFAAESPAITGTTLVVDGGQHLIPLQRDVMFVVK"
     gene            2831..3967
                     /locus_tag="VM406_11790"
     CDS             2831..3967
                     /locus_tag="VM406_11790"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HVL76687.1"
                     /translation="MSAHLPIPAPEALAASGALSDLIRAEIRSSNGWIPFARYMEMVL
                     YAPGLGYYSGGSAKLGKDGDFTTAPEITPLFGAAMAAVAEPILEQSAARILEFGAGSG
                     KLARDLLSGLAARGVVVEQYAILDLSGELRARQQETLRDFPQVEWLDRMPQAFSGLVL
                     GNEVLDAMPVQLVVKARNGWRERGVVEAETGFAFEDRPAGAEAVAQIPDVDALPEGYV
                     TEVHPQAIAFMHTVGAMVQRGAAVFIDYGFPAHEYYHPQRRQGTLMCHYRHHAHPDPF
                     YLPGLQDVTSHVDFSAIARAAQRAGLEPLCYASQAAFLIEAGIAGMLLRTDPSEARAY
                     LPQANALQKLLSPAEMGELFKVMIAGRGVVLPDGYARIDRGYRL"
     gene            4002..4196
                     /locus_tag="VM406_11795"
     CDS             4002..4196
                     /locus_tag="VM406_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HVL76688.1"
                     /translation="MIRWVLTIFVALVVFTAMLPWLEKLGVGRLPGDIRFTLFGKTVF
                     LPFASTLLLSALVVLIGRLI"
     gene            4209..4415
                     /locus_tag="VM406_11800"
     CDS             4209..4415
                     /locus_tag="VM406_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 family protein"
                     /protein_id="HVL76689.1"
                     /translation="MIRWLFVIFLALLVFTVLIPDLRRLGVGRVPGDLPVKVFGTTVL
                     LPFGSALVVFIVVLVIAEVQTLLR"
     gene            4499..6130
                     /locus_tag="VM406_11805"
     CDS             4499..6130
                     /locus_tag="VM406_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVL76690.1"
                     /translation="MSSLRSLTALIIEPHTGMRANLHNMLNLCGVTRIDHAVSSGTAI
                     RPLKSRSYDLILCEYDLGDGQDGQQLLEDLRHNKLIPLWTIFIIVTAERLYEKVVSAA
                     ELAPTDYILKPFTADMLLERIARATEKRTAFLPVYHLMEQGDLNEAIELCAAGEVRYR
                     KYATDFMRLRAELHVILGQAEQAQAIYEALLNTKAVAWARLGLAKTLFMQNRFGEAEM
                     LLAALVEESSHYMDAWDWLAKTREKTGRLDEAKDTLAGAVAISPHALRRLRKLGDMAL
                     LTGDVDVAQASFQKVVGKARHSEFKDPEDHLRLVRTLVRKGEADQAAAVVREMEKLAA
                     HSDKTPVCKAIASALMLEQAGENERAAAALDAAVTACSEAHGVSDELKMELARQCFAH
                     DRDAGAARIMRDVMSNASDEDAMAKAMEVFERSGRTDLARSVARESRRQVVEMVSGGV
                     ERARRGDYRGALDMMAAAARKLPDNPQVAFNAAVAALKHLEHLGWEARLGNQARGYIE
                     TARRLDPANPRLGGLVNMYQAILKKYEVMRVAPDG"
     gene            complement(6131..6490)
                     /gene="arsC"
                     /locus_tag="VM406_11810"
     CDS             complement(6131..6490)
                     /gene="arsC"
                     /locus_tag="VM406_11810"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081265.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HVL76691.1"
                     /translation="MLTIYHNPRCSKSREALAWMQAYAQEHGRRLEVIEYLKDPPDRR
                     RLQELQAALGCGMSDMVRSSEEAYRALGLQHADDAALLDALATHPELLQRPIVVTGDR
                     ALIARPPTLLEDFFGWR"
BASE COUNT         1159 a         2105 c         2155 g         1171 t
ORIGIN      
        1 cacgcacgca gtcgcggctg ccgcagccgg taagcgtcag ccaggtcgat gaagaattca
       61 ccatcatgcg cgaacgcgcc gagcgcgtct tcgaatacgc gctgccgcac ctggccacgc
      121 tcgcgctgtc gctcacgccg cagcagattg ccagcatcga gcgcaagttc gcgtcgaaca
      181 atgagaagtt ccgcaaggag acgctgcgca agtccccggc cgaacagcgg caggcgcgct
      241 atgagaaggt gatgaagcag gccgaatact ggttcggcaa gttcgacgcc gcgcagaagg
      301 agcgcatccg cgccgcctcc gatgcgcggc cgatggacta cgagatctgg atgcaggaac
      361 ggctgcgccg ccagcgcgag ctgatcgcgt tcctgaagaa ggtgcaggcc gaacgccccg
      421 cccggcatgc ggtggagggg atgctgcgcg cctacatggg cggcgtgctg cacaacttca
      481 cctacacgga gaacaagccg ttcttcgacg cttcccggca cggcatggcc gagatgaccg
      541 cgcagatcat caatctcgcc acacctgcgc aaaaggccca tgcgaaccgg cggctgcaaa
      601 gtctgattga cgacggccac aagctggccg gcatccgatc cggccagcag gtgtcgctgc
      661 aggagcgcta gtacggacgt atcagcagct tagtccatcg tgccgccgaa gcggatcacg
      721 ttcggcggcg gcgcgcacgg ttcctcgtcg aacgcgatgt cgccgtcggc cgaggccacg
      781 ccggtggcct tcagatcggc aaatccgaac agctcgcggt ccatcaggtg cgaaggcacc
      841 accgtcgaca gcgccgagaa gatgttctcg acccgccccg ggtgcttttt ttcccattcg
      901 cgcagcatcg ccttgatctg cttgcgctgc aaattctcct ggctgccgca caggtcgcag
      961 gggatgatcg ggaattgttt gacctgcgcc cagcgttcca tgtccgcttc cttcacgtag
     1021 gccagcggcc ggatcaccac gtggcggccg tcgtcggact gcagcttggg cggcatgccc
     1081 ttcagcttcg cgccaaagaa catgttgaga aagaaggtct cgaggatgtc atcgcggtga
     1141 tggccaagcg cgatcttggt cgcgcccagt tcgtccgcga cgcggtacag gatgccgcgg
     1201 cgcaggcgcg agcacagcga gcaggtggtc ttcccttccg ggatcagccg cttgacgatg
     1261 ctgtaggtat cctggtgctc gatatggaag ggcacgccga tcttcgtcag gtattcgggc
     1321 agcacatgct cgggaaaatt gggctgcttc tgatcgaggt tgaccgcgac gatgtcgaaa
     1381 tggatgggcg cgcgttcgcg cagcgtcagc aggatgtcga gcaggccgta gctgtccttg
     1441 ccaccggaca ggcagaccat gaccttgtcg ccgtcctcga tcatgttgaa gtcgccgatc
     1501 gcctggccga ccaggcggca cagacgcttg gacagcttgt tgttctcgaa agcgatcttg
     1561 tcggcgcgcc gcgcgtcgtt ctgttccttc aataccggat ccatgttcat gccttgatat
     1621 gtatgacttc cacgccgact gcgtcgcaat ccggatacac gtccggcttc tcggtcgaca
     1681 cgcgcaccgc gcgcaccttc gggtgcgcga gcattgcgcg cgcgacgtcg tcgcacagcg
     1741 tttcctgcag gtggatatgc ccctgcgcca tgcgccgcgc gatcgtgtcg cgcatgaaat
     1801 cgtagtcgac gacttcgtcg agctggtctt cgcgcggggt cgataccgcc agcggaatga
     1861 acagatcgac attcaccagc acccgctgct cgcccttctt ttcaaactcg tgcacgccga
     1921 tgttgatcat cacctcgtag ttgcgcagga acagccggcg gcaatcgatc aggctgggat
     1981 gggaaagggc tgaaagcatg gcgtcactct atttgacgac gaacatcacg tcgcgttgca
     2041 ggggaatcag gtgctggccg ccgtcgacga ccagcgtggt gccggtgatg gccggcgatt
     2101 cggcggcgaa gcataccgca gccgcgacat cctcgggtgt gctggagcgc ccgagcggcg
     2161 tcatcgcatg cgccttcgcg aacccttcct cggtctggtc gcccgacacc atcgtgatcc
     2221 cgggcgccac ccccaccacg cgcagcttcg gcgccagcgc ctgcgcaagc gtggtcgtcg
     2281 ccgtatgcag cgcggccttg gacagcgtgt aggaaagata atccgggttc aggttgtaca
     2341 gcttctggtc gagcaggttc accaccaccg cctgtccgcc ttccggcgtg gccgcgtgca
     2401 gcgcctgcgc cagcaacagc ggggcggtca cattggcgcg catgtggcgc tccagcagcg
     2461 cgggggaaaa gcgttgggcg tcatccggtt cgaacagcga tgcgttgttg accacgcaat
     2521 cgaggcgccc gaaggccgcg atggcgcgcg tagccaggtc gcgcgcctgc tgttcgtcct
     2581 ccaggtccgc atgcagcagg acggcgcggc gtccggtcgc cgcgatctcg tcggcgagag
     2641 actgtgcctg cgccgccgag ttgcggtaat gaatcgctac gttccagccg cgatgcgcaa
     2701 acgccagcgc gatatggcgg ccgatgcggc gcgcgcctcc ggtgacgagc actgacttgg
     2761 aagctgtatc cggcatgttc tgtccgcgta gtgaaacgaa gaaggggagc ggccgcgtac
     2821 aatacggccc atgtctgcgc acctgcccat ccccgcgccc gaagcgctcg ccgcctcagg
     2881 cgcgctgtcc gatctgattc gcgccgaaat ccgctccagc aatggatgga ttccgttcgc
     2941 gcgctacatg gagatggtgc tgtatgcacc cggcctcggg tactacagcg ggggttcggc
     3001 caagctcggc aaagacggag attttacaac cgcgcccgag atcacgccgc ttttcggggc
     3061 ggcaatggcg gccgtcgccg aaccgatact cgagcagagt gccgcgcgca tcctggagtt
     3121 cggcgccggc agcggcaagc tcgcgcgtga cctcctgagc ggactggccg cgcgcggcgt
     3181 ggtcgtcgag cagtacgcga ttctcgatct ttccggggag ctgcgcgcgc gccagcagga
     3241 gacgctgcgt gatttcccgc aggtcgaatg gctcgaccgc atgccgcagg cgttttccgg
     3301 tctcgtgctc ggcaacgagg tgctcgatgc gatgccggtg cagctcgtcg tcaaggccag
     3361 gaacggatgg cgcgagcgcg gcgtggtcga ggcggaaacc gggttcgcgt tcgaggatcg
     3421 ccctgcaggc gccgaggcgg tcgcgcagat tccggatgtc gacgcgctgc ccgaaggcta
     3481 cgtgaccgaa gtccacccgc aggcgattgc cttcatgcat accgtcggcg cgatggttca
     3541 gcgcggcgcg gccgtgttca tcgactatgg attcccggcg cacgagtact accacccgca
     3601 gcggcgccag ggcacgctga tgtgtcatta ccgccatcat gcgcatccgg atccgttcta
     3661 cctgccgggt ttgcaagatg tcacgagcca tgtcgatttc agtgcgatcg cccgcgccgc
     3721 gcagcgggcg ggactggagc cgctgtgcta cgccagccag gctgcgttcc tgatcgaagc
     3781 gggcatcgct ggcatgctgc tgcgcaccga cccgtcagaa gcgcgcgcct atctgccgca
     3841 ggccaatgcc ttgcaaaagc tgctctcgcc ggccgagatg ggcgaactct tcaaggtgat
     3901 gatcgccggg cgcggggttg tgcttccgga cggctacgcc cgcatcgacc gcggctaccg
     3961 cctgtgaatc gcggccagat caagctcgcc gaaggccgcg catgatacgc tgggtcctga
     4021 ccatcttcgt tgccctggtg gtattcaccg ccatgctgcc gtggctggag aagctcggcg
     4081 tcgggcgcct tcccggcgat atacgcttca cgctgtttgg caagacggtc ttcctgccgt
     4141 ttgcatcgac gctgctgctg tcggcgctgg ttgtcctgat cggacggctg atctgacgag
     4201 gcttgcccat gattcgctgg ctgttcgtga tctttcttgc attgctggtc ttcaccgtcc
     4261 tgattccgga cctgcgccgg ctcggcgttg gccgcgtccc cggcgacttg ccggtcaagg
     4321 tgttcggcac caccgtgctg ctgccgttcg gctcggccct ggtcgtgttc atcgtcgtgc
     4381 tcgtgatcgc cgaagtgcag acgctgttgc gatagacttg catttcttca aggcaatggt
     4441 atgttgccag cacctcgagc ccgacgccgg gctccttgaa gccgtccgaa cgccagccat
     4501 gtcatcgctt cgaagcctga ccgcattgat catcgagccc catacgggca tgcgcgcgaa
     4561 tctgcacaac atgctcaacc tgtgcggcgt gacgcggatc gaccacgcgg tcagctccgg
     4621 caccgcgatc cgcccgctga agagccgctc ctacgacctg atactgtgcg aatacgacct
     4681 cggcgacgga caggacggcc agcagttgct cgaagacctg cgccacaaca agctcatccc
     4741 gctgtggacg atcttcatca tcgtcaccgc cgaacgcctg tacgaaaagg tcgtcagcgc
     4801 ggctgaactg gcgcccaccg actacatcct caagcccttc accgccgaca tgctgctcga
     4861 gcgcattgcg cgcgcgaccg agaaacgcac cgccttcctg cccgtgtacc acctgatgga
     4921 acaaggcgac ctgaacgaag ccattgaact gtgcgcggcg ggcgaagtgc ggtaccggaa
     4981 gtacgcaacc gatttcatgc gcctgcgcgc cgagctgcac gtgatcctcg ggcaggcgga
     5041 gcaggcgcag gccatctacg aggccctgct gaacacgaag gcggtcgcat gggcgcggct
     5101 tgggttggca aagacgctgt tcatgcagaa caggttcggc gaggctgaaa tgctgctcgc
     5161 cgcgctggtc gaggaaagca gccactacat ggacgcctgg gactggctgg caaagacccg
     5221 cgagaagacg ggccgtctgg acgaggcgaa ggacacgctg gccggcgccg tcgccatctc
     5281 gccgcacgcg ctgcgtcgcc tgcgcaagct aggcgacatg gcgcttctga ccggcgatgt
     5341 ggacgtcgcc caggcttcgt tccagaaggt cgtcggcaag gcgcgccatt ccgagttcaa
     5401 ggatcccgag gaccacctgc gcctggtgcg tacgctggtc aggaaggggg aggcggatca
     5461 ggcggccgcg gtggtgcggg aaatggagaa actggccgcg cattcggaca agacaccggt
     5521 ttgcaaggcc atcgcgtccg cgctgatgct cgagcaggcc ggcgagaacg aacgcgccgc
     5581 ggccgccctt gacgccgctg tgacggcctg cagcgaagcg catggcgttt ccgacgaact
     5641 gaagatggag ctcgcgcggc aatgctttgc tcatgatcgc gacgccggcg ccgcccgaat
     5701 catgcgcgac gtcatgagca acgcttccga cgaggacgcg atggcgaagg cgatggaggt
     5761 gttcgagcgg tccgggcgca cagatctggc gcgcagcgtg gccagggaga gccgtcgtca
     5821 agtggtggaa atggtttccg gcggcgtcga gcgtgctcgg cgaggtgact accggggggc
     5881 cctcgacatg atggcggcgg cagcgcgcaa gttgccggac aatccgcaag tcgcattcaa
     5941 cgccgcggtc gccgccctca agcacctcga gcatctcggc tgggaagcgc gcctcggcaa
     6001 tcaggcccgc ggctatatcg agacagcgcg gcgtctggat cctgccaatc cccggcttgg
     6061 cgggctggtc aacatgtacc aggccatcct gaaaaaatac gaggtcatgc gcgttgcgcc
     6121 ggacggctga tcagcgccag ccgaaaaagt cctcgagcag ggtaggcggg cgggcgatca
     6181 gtgcccggtc gccggtcacg acgatagggc gctgcagcaa ctccgggtga gtggcgagcg
     6241 cgtcaagcag ggctgcgtcg tcggcatgct gcaagccgag cgcgcggtat gcctcttcgc
     6301 tgctgcgcac catgtcgctc atcccgcagc ccagtgccgc ctggagttcc tgcaaacgcc
     6361 tgcggtccgg cggatccttc aggtattcga tcacttcgag gcgcctgcca tgttcctgag
     6421 cgtacgcctg catccaggcc agcgcttcgc gcgacttcga gcagcgcgga ttgtggtaga
     6481 tggtgagcat catgatacga aggcgggaat tgaaaggatc gcagtgtaat gcagcctcaa
     6541 gggacgatgg gtttcttcac gctatactcg cttgatccta ggaattgaac
//
LOCUS       DATLGF010000386         7758 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_62000,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000386 DATLGF010000000
VERSION     DATLGF010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 7758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7758
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62000"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /gene="secD"
                     /locus_tag="VM406_11815"
     CDS             <1..772
                     /gene="secD"
                     /locus_tag="VM406_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HVL76692.1"
                     /translation="NGDGGRRMREATRGRIGRLMAIVLFERGQGEVLTVATIQDELGS
                     RFQITGMGTPQAANDLALLLRAGSLAAPMEIIEERTIGPQLGADNIAKGFNSVLYGFA
                     ALIIFMIVYYHLFGFFSVVALTVNLMLLIGVLSMLQATLTLPGMAAIAFTLGMAIDAN
                     VLINERIREELRAGASPQMAIATGFDRAWATILDSNITTLIAGLALLIFGSGVVRGFA
                     VVHTIGILTSIFSAVFVSRGIINLWYGRKKKLTTLAIG"
     gene            833..1777
                     /gene="secF"
                     /locus_tag="VM406_11820"
     CDS             833..1777
                     /gene="secF"
                     /locus_tag="VM406_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HVL76693.1"
                     /translation="MELFRIKKDIPFMRHALLLNAFSAAAFIAAVFFIFYKGLNLSIE
                     FTGGTVMEVTYSQPADLPRIRGAIEELGFTDTQVQSFGTAQDVLIRMPTVPGITSAAQ
                     SEKVMGALRAQQNDVQLQRVEYVGPQVGEELAQDGLMALAMVIIGIMVYLAVRFEWKF
                     AVAAIIANLHDVVIILGFFAFFQWEFSLTVLAAVLAVLGYSVNESVVVFDRVRETFRD
                     RRNNKMSTHEVIDHAITRTMSRTIITHTSTEIMVVSMLIFGGPTLFYFSLALTIGIVF
                     GIYSSVFVAAAVAMWLGVKREDLVKPIKEKDQTDGAVV"
     gene            1864..2541
                     /locus_tag="VM406_11825"
     CDS             1864..2541
                     /locus_tag="VM406_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HVL76694.1"
                     /translation="MKKIRVWDLPLRLFHWTLAALLVAAWVTQYIGGNAMDWHFRIGY
                     AVLVLILFRIVWGFIGPRYARFSSFLYGPSAIAGYLRGHPSQRPGHNPLGSLSVFALI
                     GVVFAQALSGMFTNDDIFFTEGPLARYISKDLSDWLTWFHRDVSVVALYVMVALHLAA
                     IAWYVVVKRKRLIRPMFTGDQEVEEPMPGIEAARDDWKTRLLAVTILGGLAALMIWIV
                     TLPRTYL"
     gene            complement(2638..3087)
                     /locus_tag="VM406_11830"
     CDS             complement(2638..3087)
                     /locus_tag="VM406_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVL76695.1"
                     /translation="MKHAFAGFALAALAALPLAAQAQFAKVEDAVKYRQSAFALMGAH
                     MGRIGAVVKGEQPYDKAAVETDAAIIEMMSRLPWHAFPAGSETNSRARPEIWREQDKF
                     KGLSEKMQAEVQKLSVAARGGDLAQIRSAMGAVGQSCRACHDNFRAR"
     gene            complement(3217..4584)
                     /gene="purB"
                     /locus_tag="VM406_11835"
     CDS             complement(3217..4584)
                     /gene="purB"
                     /locus_tag="VM406_11835"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HVL76696.1"
                     /translation="MPLSPLSALSPLDGRYAAKTDALRPLLSEAGFMHHRVKVEIAWL
                     QALAAAGFAEIKPFSPAAAAQLDKLAADFSEADAARIKEIEAVTNHDVKAVEYWLKEK
                     VKDVPELVAASEFIHFACTSEDINNTSHGMMLKSARDSVLLPALQAVIARLTELAHAN
                     ADLPMLSRTHGQPASPTTLGKELANVVARLKRAEARISSVEILGKMNGAVGNYNAHLS
                     AYPEFDWQAFSRQVIEERLGLSFNPYTIQIEPHDYMAELFDAIARANTILLDLNRDIW
                     GYISLGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNLGLANAMLKHLSEKLPVSRWQ
                     RDLTDSTVLRNIGVGFGYALLAYDSCLRGLNKLEVNPQRLAEDLDATWEVLAEPVQTV
                     MRRYGIENPYEQLKELTRGKGISREALQDFVSGLAIPQEAKDRLLAMTPASYTGLARQ
                     LANEI"
     gene            4760..5368
                     /locus_tag="VM406_11840"
     CDS             4760..5368
                     /locus_tag="VM406_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HVL76697.1"
                     /translation="MKLIGSHASPYVRKVRVAMAEKKLDYELIVEDVWATGTTIQQSN
                     PLGKVPCLVMEDGGAMFDSRVIVEYLDTLSPVGKLIPPNGRERAEVKCWEALADGVVD
                     AAVLIRLERTQRPPHLQSEEWIRRQQSKIDAGLKAMASGLKETPYCTGNHYTLADVAV
                     GCTLGWLSFRFPEIDWRDRHPNLAKLYDKLAERPSFKETVPR"
     gene            complement(5428..5904)
                     /gene="bfr"
                     /locus_tag="VM406_11845"
     CDS             complement(5428..5904)
                     /gene="bfr"
                     /locus_tag="VM406_11845"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HVL76698.1"
                     /translation="MKGDPNIIRLLNAQLTNELTAINQYFLHARMYRHWGFEKLGKKE
                     YDESIGEMKHADDLINRILMLDGLPNLQALNKLLIGESVPEMLECDLKLEQGAQQTIK
                     DGIKACEAAGDYVSREIFEDILEDTEEHIEWLETQLDLINKVGLQNYLQAQMGESS"
     gene            complement(6033..6248)
                     /locus_tag="VM406_11850"
     CDS             complement(6033..6248)
                     /locus_tag="VM406_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HVL76699.1"
                     /translation="MIVCVCFNVSDSKIRLAADQGHTSMPALREELGVGTCCGKCHSC
                     AKSVLRTHLEAGMREPQAVPADAALAA"
     gene            complement(6312..6632)
                     /locus_tag="VM406_11855"
     CDS             complement(6312..6632)
                     /locus_tag="VM406_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76700.1"
                     /translation="MATDAIDPRPPGRADCRGQAALMLSSLLHLMSHYSLRRQRGESI
                     DRLTATIERHLQVLAEWPDLEPVLRATCAELGAQWRQMADSAAQPERQSLLMRLVAPA
                     RGCA"
     gene            complement(6801..>7758)
                     /gene="mnmA"
                     /locus_tag="VM406_11860"
     CDS             complement(6801..>7758)
                     /gene="mnmA"
                     /locus_tag="VM406_11860"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HVL76701.1"
                     /translation="QDWIDAASVADVVGVDIEAVNFAAEYKDRVFAEFLREYQAGRTP
                     NPDVLCNAEIKFKAFLDHAMKLGADRIATGHYARVREVDGRFELLKAVDASKDQSYFL
                     HRLNQAQLAKTLFPLGEIPKTEVRRIAEKIGLPNAKKKDSTGICFIGERPFREFLNRY
                     LSHRPGPMKTPDGTIVGEHIGLSFYTLGQRKGIGLGGMKAYQSEAGTSQAWYVAGKDV
                     ATNTLYVCQGHDHPWLLGERLEASQESWIAGARPAPGSYSAKTRYRQADVPCSLSDAA
                     PGSFSLNFPAPQWALTPGQSAVLYDGEVCLGGGIIDTAHRLS"
BASE COUNT         1349 a         2363 c         2532 g         1514 t
ORIGIN      
        1 caacggcgac ggcggtcgcc gcatgcgcga agccacgcgc gggcgcatcg gccgcctgat
       61 ggcgatcgtg ctgttcgagc gcggccaggg cgaagtgttg acggtggcaa ccatccagga
      121 cgaactcggc tcgcgcttcc agatcaccgg catgggcacg ccgcaggccg ccaacgatct
      181 cgcgctgctg ctgcgcgcgg gctcgctggc cgcaccgatg gaaatcattg aggagcgcac
      241 gatcggcccg cagctgggcg ccgacaacat tgccaagggc ttcaattcgg tgctgtacgg
      301 cttcgccgcg ctgatcatct tcatgatcgt ctactaccac ctgttcggct tcttcagcgt
      361 ggtcgcgctt acggtcaacc tgatgctgct tatcggtgtg ttgtcgatgc tgcaagccac
      421 gctcacgctg cccggcatgg cggccatcgc cttcacgctg ggcatggcga tcgatgcgaa
      481 cgtgttgatc aacgagcgca tacgcgagga gctgcgcgcg ggtgcctcac cgcagatggc
      541 gatcgccacc ggcttcgacc gggcttgggc gacgattctc gattccaaca tcaccacgct
      601 gattgccggc ctcgcgctct tgatcttcgg ttccggcgtg gtgcgtggtt tcgcggtcgt
      661 gcacacgatc ggcatcctga cctcgatctt ctcggcggtg ttcgtctcgc gcgggatcat
      721 caatctgtgg tacgggcgca agaagaagct caccacgctg gcaatcggtt gaaccgcgcg
      781 caattgacgc aggcaggcgg cgcaatgcgc ccctgccatt gaaaggaaag acatggagct
      841 gttccggatc aagaaagaca taccgttcat gcggcacgcg ttgctcctca acgcgttctc
      901 cgcggcggcc ttcatcgcgg cggtgttctt cattttttac aagggcctga atctctcgat
      961 cgagttcacg ggcggcacgg tgatggaagt cacctattcg cagccggccg acctgccgcg
     1021 cattcgcggt gcgatcgagg agctgggctt caccgacacc caggtgcaga gcttcggtac
     1081 cgcccaggac gtgctgatcc gcatgccgac ggtcccggga atcacctcgg ccgcgcagag
     1141 cgagaaggtg atgggcgcgc tgcgcgccca gcagaacgac gtgcagctgc agcgggttga
     1201 gtatgtcggc ccgcaggtcg gcgaggagct ggctcaggac ggcctgatgg cgctggcgat
     1261 ggtgatcatc gggatcatgg tctacctcgc ggtgcgcttc gaatggaaat tcgcggtggc
     1321 cgcgatcatc gcgaatttgc acgacgtcgt gatcattctc ggcttcttcg cgttcttcca
     1381 gtgggagttc tcgctgaccg tgctggcggc ggtgctggcc gtgctcggct actcggtcaa
     1441 cgagtcggtc gtggtgttcg atcgcgtccg cgaaaccttc cgcgaccgcc gcaacaacaa
     1501 gatgtcgacg catgaggtca tcgaccacgc gatcacgcgc acgatgtcgc gcacgatcat
     1561 cacccatacg tcgacggaaa tcatggtcgt ctcgatgctg atcttcggcg gcccgaccct
     1621 gttctacttc tcgttggcgc tgacgatcgg catcgtgttc ggcatctatt cgtcggtgtt
     1681 cgtcgcggca gcggtcgcga tgtggctggg cgtcaagcgc gaggacctgg tcaagccgat
     1741 caaggaaaag gatcagaccg acggcgcggt cgtctgaatc tgcccgcatc gcggaaaagc
     1801 cggctccatc gagccggttt tttttcgtct acactttccc cgctggtcaa cgcgggagga
     1861 acaatgaaga aaatacgcgt ctgggatctg ccgctgcgcc tgtttcattg gacgttggcg
     1921 gcgctgctcg tcgccgcatg ggtgacgcag tacatcggcg ggaacgcgat ggactggcat
     1981 ttccgcatcg gctatgcggt gctggtgctc atcctgttcc gcatcgtctg gggcttcatc
     2041 gggccgcgtt atgcgcgctt ctccagcttt ctctacgggc cctcggccat cgccggctac
     2101 cttcgcggac acccgtcgca gcggccgggc cacaatcctc tgggcagtct gtcggtgttc
     2161 gcgttgatcg gagtggtatt cgcgcaggcc ctgtccggca tgttcaccaa tgacgacatc
     2221 ttcttcaccg aggggccact ggcgcgctac atcagcaagg acctgtcgga ctggctcacc
     2281 tggttccatc gcgacgtgag cgtcgtcgcg ctgtatgtga tggtcgcact gcacctggct
     2341 gcgattgcct ggtatgtggt ggtgaagagg aagcgcctga tcagaccgat gttcaccggc
     2401 gaccaggagg tggaggagcc gatgcccgga atcgaagctg cgcgcgacga ctggaagacg
     2461 cggctgctcg ccgtgacaat actgggcggg ctcgcagcgc tgatgatatg gatcgtcacg
     2521 ctgccgcgga cttacctctg acaatcgcgc ttgcgcggaa atcaaggtga agtcttgaac
     2581 cacggcgggg acggcgaggg tcttttttcg ccatgcccgc catggtgaag accaatatca
     2641 gcgagcgcgg aaattatcgt gacacgcgcg gcaggactgg ccgaccgcgc ccatcgcgct
     2701 gcggatctgc gccagatcgc cgccgcgagc ggcaactgaa agcttttgca cttcggcctg
     2761 catcttttcc gacaatccct tgaacttgtc ctgttcgcgc cagatctcgg ggcgcgcccg
     2821 gctgttggtt tccgaaccgg cggggaaggc atgccatggc aggcggctca tcatttcgat
     2881 gatggccgca tcggtctcga ccgccgcctt gtcgtacggc tgttccccct tgaccaccgc
     2941 gccgatgcgg cccatgtggg cgcccatcag cgcaaaggcg gattggcggt acttgacggc
     3001 gtcctcgacc ttggcgaatt gcgcctgcgc cgccagcggc agtgcggcga gggcggcaag
     3061 cgcgaaacct gcaaatgcat gcttcatgag agctccttga aatgacacgg cgggaatgcc
     3121 ccacaggcga cggggaaact tctgcgaagc aatgtagcag aaaaaaaacg gcgttgccga
     3181 aggagcaacg ccgtatgtgg aaacgcgaag cgcggctcag atttcgttag ccagctggcg
     3241 cgcgaggccg gtgtacgaag cgggggtcat cgcgagcagg cggtccttgg cttcctgcgg
     3301 aatggccagg ccggagacga agtcctgcag ggcttcgcgc gaaatgccct tgccgcgggt
     3361 gagctccttc aactgctcgt acgggttctc gatgccgtag cggcgcatca cggtttgcac
     3421 aggctcggcc agcacttccc aggtcgcgtc caggtcctcg gccaggcgtt gcggattgac
     3481 ttcaagcttg ttcagcccgc gcaggcagct gtcgtaggcg agcaacgcgt aaccgaagcc
     3541 cacgccgata ttgcgcagca cggtggaatc ggtcaggtcg cgctgccagc gcgagaccgg
     3601 gagcttctcg gacaggtgct tgagcattgc attggccagg ccgaggttgc cctccgagtt
     3661 ctcgaagtcg atcggattga ccttgtgcgg catcgtcgac gagccgattt cgccggcctt
     3721 ggtcttctgc ttgaaatagc cgagcgagat atagccccag atgtcccggt tcagatcgag
     3781 caggatggta ttggcccgcg cgatcgcgtc gaacagctcg gccatgtagt cgtgcggctc
     3841 gatctggatc gtgtacggat tgaaggacag tcccaggcgc tcctcgatca cctgcctgga
     3901 aaacgcctgc cagtcgaatt cgggataggc ggacaggtgg gcgttgtagt tgccgaccgc
     3961 gccgttcatc ttgccgagga tctcgacgct ggagatgcgg gcttcggcgc gcttcaggcg
     4021 cgccactaca ttcgccagtt ccttgccgag ggtggtcggg ctggccggtt ggccatgggt
     4081 gcgcgagagc ataggcaggt cggcgttggc gtgcgccagt tcggtcagcc tggcgataac
     4141 cgcctgcagt gccggcagca ggacactgtc ccgcgccgac ttgagcatca tgccgtgcga
     4201 ggtgttgttg atgtcttccg acgtgcaggc gaagtggatg aactccgatg ccgcaaccag
     4261 ttccgggacg tccttgacct tctccttcag ccagtattcg accgccttga cgtcgtggtt
     4321 ggtgaccgct tcgatttcct tgatgcgggc tgcgtcggct tcggaaaaat cggcggccag
     4381 cttgtcgagc tgcgcggccg cagccggcga aaatggcttg atctcggcga aaccggcagc
     4441 ggccaaagcc tgcaaccatg cgatttcaac cttgacgcgg tgatgcatga aacccgcctc
     4501 ggacagcagc gggcgcaggg catcggtttt cgcggcgtag cggccgtcca gcggcgagag
     4561 ggcggagaga ggggagagag gcatggcggg aactgcgagg aggttggaaa acggtgcggg
     4621 cgcgggatcc ggcgccgcaa agtgcgcatt ctaccattcg ccccatccgg gcaatccggc
     4681 gccgggccgg cggacggccg cattcgggag gtgacatacc tcaatgttat aatcgcaacc
     4741 catcctacga tacgaagcta tgaaactgat cggttcccat gccagcccct acgtccgcaa
     4801 ggtgcgcgtc gcgatggcgg agaagaagct cgactatgag ctgattgtgg aagacgtctg
     4861 ggcgacaggc acgaccatcc agcagtccaa cccgctcggc aaggtgccgt gcctggtgat
     4921 ggaagacggc ggagcgatgt tcgactcgcg cgtgatcgtc gaatacctcg ataccctttc
     4981 cccggtcggc aagctcattc cgccaaacgg ccgcgagcgc gccgaagtga agtgctggga
     5041 agcgctggcc gacggcgttg tcgacgcagc agtcctgatc cgcctcgagc gcacccagcg
     5101 tccgccgcac ctgcaaagcg aagaatggat caggcggcag cagtccaaga tcgatgccgg
     5161 gctgaaggcg atggcgtcag gtttgaagga aacgccgtac tgcaccggca accactacac
     5221 gctggccgac gtcgcggtcg gctgcacgct gggctggctg tcgttccggt ttccggagat
     5281 cgactggcgc gaccgtcatc cgaatctcgc caagctttat gacaagctcg cggaacgccc
     5341 gtccttcaag gagacggtgc cgcgctgagc aagcggacca aacaaaaagg cagcctcggc
     5401 tgcctttttg tttggttgaa acgggcgtca ggaggattcg cccatttgcg cctggaggta
     5461 gttctgcagg cccaccttgt tgatcaggtc aagctgggtc tccagccatt cgatgtgttc
     5521 ctcggtgtct tccagaatgt cctcgaaaat ctcccgcgac acatagtcgc cggccgcctc
     5581 acaggccttg atgccgtcct tgatcgtctg ctgcgcaccc tgttccagct tcagatcgca
     5641 ttccagcatt tccggtaccg attctccgat caggagcttg ttgagcgcct gcaggttcgg
     5701 cagcccgtcg agcatcagga tgcggttgat caggtcgtcg gcatgcttca tctcgccgat
     5761 cgactcgtcg tattccttct tgccgagctt ctcgaagccc cagtggcggt acatgcgggc
     5821 gtggaggaag tactgattga tcgcggtgag ctcgttggtg agctgggcgt tgagcaggcg
     5881 gatgatgttc gggtcgcctt tcatcgggct cctccatgga tgtgcgggaa agccggagcg
     5941 ggtggtccgg gacattgcgc atcataacgc aggagagccg ctcacgttgg cattccggcc
     6001 acacgccacg cccgaatgca agagcgcggg cgtcaggccg ccaaggccgc gtcggcaggc
     6061 acggcttgcg gctcgcgcat gcccgcttcg aggtgggtgc gcagaaccga tttcgcgcac
     6121 gagtggcatt ttccacagca ggtgccgaca cccaattctt cgcgcagcgc cggcatgctg
     6181 gtatgaccct gatccgccgc caggcggatc ttcgagtcgg agacgttgaa gcagacacag
     6241 acaatcatca aatcacctga actggtcgat gcggcgaaca atggatcaaa ggaaccggcc
     6301 gggacgcccg cttatgcgca gccgcgcgcg ggtgcgacca gacgcatcaa caggctctgc
     6361 cgttccggct gcgccgcgct gtccgccatc tgtcgccact gcgccccgag ctccgcacag
     6421 gtcgcccgca gcaccggttc caggtccggc cattcggcca ggacttgcag gtgccgctcg
     6481 atggtcgccg tcaagcggtc gatcgactcg ccgcgctggc gacgcaggct gtagtgcgac
     6541 atcaggtgca gcagcgaaga cagcatcagt gccgcctgtc cccgacagtc tgcgcgcccc
     6601 ggagggcgcg ggtcgattgc gtcggtagcc atcatctgat ccgttgctct ttcataaatg
     6661 agaacgattc gtattcagat tattggtgat gtggcagacg aatgcaagcg atatttttgg
     6721 ctgtgttgta agcgggcgtg agggcgctgg cgtcccgctt tcgcgggtcg ccggcgctga
     6781 atgacctcaa cgagcggaga tcaggagagg cggtgggcgg tgtcgatgat gccgccgccc
     6841 aggcagacct cgccgtcgta gaggacggcg gattgccctg gcgtgagcgc ccactgcggg
     6901 gcgggaaaat tcagcgagaa gctgccaggt gcggcatcgc tcagactgca tggcacgtcg
     6961 gcctgccggt agcgcgtctt cgccgagtag ctgccggggg ccgggcgcgc gccggcgatc
     7021 cagctctctt gagacgcttc gagccgctcg ccaagcagcc agggatggtc gtgcccctgg
     7081 cacacataca gggtgttcgt cgccacgtcc ttgccggcga cgtaccaggc ttggctggtg
     7141 ccggcctcgc tctggtaagc cttcatcccg cccaggccga tgcccttgcg ctggccgagc
     7201 gtgtaaaaac tcaggccgat atgctcgcct acgatggtgc cgtcgggggt tttcatcggt
     7261 cccggacggt gcgacaggta gcggttcagg aattcgcgga acggccgctc gccgatgaag
     7321 cagatgccgg tcgaatcctt cttcttcgcg ttcggcaggc cgatcttttc cgcgatccgg
     7381 cgcacctcgg tcttgggaat ctcgccgagc ggaaacagcg ttttggcgag ctgggcctgg
     7441 ttcagccggt gcaggaaata gctctggtcc ttgcttgcat cgaccgcctt gagcagttcg
     7501 aagcggccgt cgacctcgcg cacgcgcgcg tagtgaccgg tggcaatccg gtcggcgcca
     7561 agcttcatcg catgatcgag gaaggccttg aacttgatct cggcgttgca gagcacgtcc
     7621 gggttcgggg tgcggccggc ctggtattcg cgcaggaatt cggcgaacac gcggtccttg
     7681 tattcggcgg cgaagttgac cgcctcgatg tccacgccga cgacatcggc gacactggcc
     7741 gcatcgatcc agtcctgg
//
LOCUS       DATLGF010000387         5150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_62175,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000387 DATLGF010000000
VERSION     DATLGF010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62175"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VM406_11865"
     CDS             <1..184
                     /locus_tag="VM406_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76702.1"
                     /translation="LLQLASKYIGHTFGCVSLTLEMPFKDNAMLPDPATGWDGRRSMR
                     LGAAILQPMLQAVEGS"
     gene            186..650
                     /locus_tag="VM406_11870"
     CDS             186..650
                     /locus_tag="VM406_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HVL76703.1"
                     /translation="MGMEIERKFLVRDLRWKSMAQGVLLRQGYLSSAPERVVRVRIEG
                     DRALLTIKGRNIGAARGEWEYPVPLFDAQTFLDELCERPIIEKLRYRIPLHGLVWEVD
                     EFLGDNAGLIVAEVELHHPDQAFDKPEWVGEEVTHDPRYFNANLLRHPYSKW"
     gene            complement(661..1911)
                     /locus_tag="VM406_11875"
     CDS             complement(661..1911)
                     /locus_tag="VM406_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2863 family protein"
                     /protein_id="HVL76704.1"
                     /translation="MRRFSKTSPNKLSSDAHRLVCLAQGMAQAASRLEERQWENRLDL
                     QIHKLLKGAQQDAIDGALEYLFSSDIAAYDALLEAVEAGSESCSVEREGEQYHALLIA
                     LPILAWTRFSIASGTIPDQMRTTLCAHLHGHLLADGTCLALAPTLFSIDQLPRTHVET
                     FALTQRMAQAAVKGAGIKPLANAPETAPFLADTRYLLAAVAAPAGQPLFCWQAAMQPA
                     ERATAFAQWRAQATPNIASLLPGCGVELLMPEAYYVACREADRQIRPASIRAAIHYLT
                     HTLGVDANGLRAVIGGFAEEPLSGRVDEYRVGFTLRQNPEVIYGVVWPLYGQEDEEES
                     DIAPSFEALTTPEILEAESRTPLEEITALLRACGITHIKRHAEHFPLEFCDDCGAPLY
                     PDPDGELVHAEMPEDAPAGPGHFH"
     gene            complement(2093..4804)
                     /gene="acnA"
                     /locus_tag="VM406_11880"
     CDS             complement(2093..4804)
                     /gene="acnA"
                     /locus_tag="VM406_11880"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HVL76705.1"
                     /translation="MSRNTLNTLKEFKISDSRKGKFYSLPALEKKLGVNISRLPVSIR
                     IVLESVLRNCDGKKVTEEHVRQLANWKPNAQRVDEIPFVVARVVLQDFTGVPLLADLA
                     AMRGVAQKMGKKPKNIEPLVPVDLVVDHSVQIDHFREKKALDLNMKLEFQRNNERYQF
                     MKWGMQAFDTFGVVPPGFGIVHQVNLEYLARGVHKKEGVFYPDTLVGTDSHTTMINGI
                     GVVGWGVGGIEAEAGMLGQPVYFLTPDVVGVHLTGALREGVTATDLVLTITELLRKHK
                     VVGKFVEFFGEGTESLSLTDRATIGNMAPEYGATMGFFPVDDATIDYFRGTGRTKAEI
                     DAFEAYFKAQKMYGIPKKGEVDYSAVVELDLGSVAPSLAGPKRPQDRIEIGNVKSTFT
                     ELFSKPVSENGFNKKAEDLGAEYDTADGVKLHNGDVLIAAITSCTNTSNPSVLLGAGL
                     LAKKAVEAGLKVAPHIKTSLAPGSRVVTKYLEAAGLLPYLEKLGFGVTAYGCTTCIGN
                     AGDLTPALNEAIVKNDVVAAAVLSGNRNFEARIHPNIRANFLASPPLVVAYAIAGNVR
                     RDLMTEPVGKGKGGRDIYLGDIWPTSEEIAKLMKYAMNAKIFKENYADVEGAPGKLWE
                     QIKGVAKGEVYNWPKSTYIAEPPFFEDFGMEPKAAAGGISGARALGVFGDSITTDHIS
                     PAGSIKETSPAGKWLLQNGVLKADFNSYGSRRGNHEVMMRGTFANVRIKNLMIPPKAD
                     GSRVEGGLTIHQPSGEQMSIYDAAMKYIDDGVPTMVFAGEEYGTGSSRDWAAKGTQLL
                     GVKAVIARSFERIHRSNLVGMGVLPLQFLNDDSVHSLGITGNETFDLKGIEGEIKPQQ
                     KATLVINRADGSKQEVQLLLRIDTPIEVDYYKHGGILPFVLRQLLAA"
     gene            complement(4891..>5150)
                     /locus_tag="VM406_11885"
     CDS             complement(4891..>5150)
                     /locus_tag="VM406_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76706.1"
                     /translation="YDCDLGRLTIYRKADDNQQIALQWNKRTHQMTRKETSTGAERYE
                     SADSGLVWIGIPAKGMLLDSKQGRQVANDCRNETQKKQARN"
BASE COUNT          827 a         1601 c         1713 g         1009 t
ORIGIN      
        1 cctgctgcaa ctggcgtcga agtacatcgg tcacaccttc gggtgcgtgt cgctcacgct
       61 ggagatgccg ttcaaggaca acgccatgct tcccgacccg gccacgggct gggatggccg
      121 gcgcagcatg cgcctgggtg cggcgatcct gcagccgatg ctgcaggccg tggagggcag
      181 ctgaggtggg gatggaaatc gaacgcaagt ttctggtgcg ggacctgcgc tggaagtcga
      241 tggcgcaggg cgtgctgctg cgccagggct acctgtcctc ggcgccggaa cgggtggtgc
      301 gcgtgcgtat tgaaggcgac cgtgcgctgc tcacgatcaa gggccgaaat atcggcgccg
      361 cgcgcggaga gtgggagtat ccggtccccc tgtttgatgc ccagaccttc ctcgacgaac
      421 tgtgcgaacg accgatcatc gagaagctgc gctaccgcat tccgctgcat ggcctggtgt
      481 gggaagtcga cgagttcctc ggcgacaacg ccggcctgat cgtggccgag gtggaactgc
      541 accacccgga ccaggctttc gacaaaccgg agtgggtggg tgaggaggtg acgcacgacc
      601 cacgttattt caacgcgaac ctgctgcgcc acccgtacag caagtggtga cgtcagccgg
      661 ttagtggaag tggccgggcc cggcgggggc gtcttccggc atttccgcgt gcaccagttc
      721 accatccgga tccgggtaga gcggggcgcc gcagtcgtcg cagaactcca gcggaaaatg
      781 ctcggcgtgg cgcttgatgt gggtgatccc gcaggcccgc agcagggcgg tgatctcctc
      841 cagcggcgtg cgtgactcgg cctcgaggat ctcgggcgtg gtcagtgcct cgaaactggg
      901 tgcgatgtcc gactcttcct cgtcctcctg gccgtacagc ggccatacga ccccatagat
      961 cacctccggg ttctggcgca gcgtgaagcc gacccggtac tcgtccacgc gtccggacag
     1021 cggttcctcg gcaaagccgc cgatcaccgc acgcagaccg ttcgcatcaa caccgagcgt
     1081 gtgcgtcagg taatggatcg ccgcccggat cgaagcgggc cggatctggc gatccgcctc
     1141 gcggcaggcc acatagtagg cctcgggcat cagcagctcg acgccgcagc ccggcagcag
     1201 ggaggcaatg ttcggcgtgg cctgcgcgcg ccattgcgca aacgcagtcg cccgttctgc
     1261 gggctgcatt gcggcctgcc agcagaacag cggctggccg gcaggcgcgg ccaccgccgc
     1321 cagcagatag cgggtatcgg cgaggaaggg agcagtctcg ggcgcattgg cgagcggctt
     1381 gatgccggcg cccttgacgg ccgcctgggc catgcgctgc gtcagcgcga acgtttccac
     1441 atgggtgcgc ggcaactggt cgatagagaa cagcgtcggg gccagggcca ggcaggtgcc
     1501 gtcggcgagc aggtgaccat gcaggtgcgc gcacaaggtg gtgcgcatct ggtccgggat
     1561 cgtgcccgat gcgatcgaga aacgggtcca ggccaggatg ggcagggcga tcagcagcgc
     1621 atggtactgc tcgccctcgc gctccaccga gcaggattcg ctgccggcct cgacggcttc
     1681 gagcagggcg tcgtaggcgg caatatcgga gctgaacagg tattcgagcg cgccatcgat
     1741 ggcatcttgc tgcgcaccct tgagcagctt gtggatctgc aggtcgagcc ggttttccca
     1801 ctggcgttct tccaggcggc ttgcggcctg cgccatgccc tgcgcgaggc agacgagccg
     1861 gtgcgcatcg gaggagagct tgttcgggga agtcttggag aaacggcgca tggtgcttcg
     1921 gacagtacgg aagactcgta ttgtagttga ttcaattcgc cccgcatccg cgcggatcgc
     1981 agctcctgcg ccgaagcagg cgagattttg caagggaaat gaaaaaggcc gcatgcgtga
     2041 gcatgcggcc ttcgtcggcc aggcttgagg gcgcgggccc tccttgccct gcttaagctg
     2101 cgagcagctg gcgcagcacg aacggcagga tgccgccgtg cttgtagtaa tcgacctcga
     2161 tcggagtgtc gatgcgcaac agcaactgta cttcctgctt gctgccgtcg gcgcggttga
     2221 tcaccagcgt ggccttctgc tgcggcttga tctcgccctc gatgcccttc agatcgaagg
     2281 tttcattgcc tgtgatgccc agcgaatgga cgctgtcatc gttgaggaac tgcagcggca
     2341 gcacgcccat gccgaccagg ttcgaacggt ggatgcgctc gaacgaacgc gcgatcaccg
     2401 ccttcacgcc gagcagctgg gtgcccttgg ccgcccagtc gcgcgaggag ccggtgccgt
     2461 actcttcgcc tgcgaacacc atggtcggca cgccgtcgtc gatgtacttc atcgctgcgt
     2521 cgtagatcga catctgctcg ccgctcggct ggtggatggt caggccgccc tcgacgcgcg
     2581 aaccgtcggc cttcggcggg atcatcaggt tcttgatgcg cacgttggca aaggtgccgc
     2641 gcatcatcac ctcatggttg ccgcggcgcg agccgtagga gttgaagtcg gccttgagca
     2701 cgccgttttg cagcagccac ttgccggcgg ggctggtttc tttgatggag ccggccggcg
     2761 agatgtggtc ggtggtgatc gagtcgccga acacgcccag cgcgcgggcg ccggagatgc
     2821 cgccggcggc ggctttcggt tccatgccga aatcctcgaa gaacggcggc tcggcgatgt
     2881 aggtcgactt cggccagttg tacacctcgc ccttggccac acccttgatc tgctcccaca
     2941 gcttgcccgg ggcgccttcg acgtcggcgt agttctcctt gaagatcttc gcgttcatcg
     3001 cgtacttcat cagcttggcg atttcttccg aggtcggcca gatgtcgccc aggtagatgt
     3061 cgcggccgcc cttgcccttg ccgacgggtt cggtcatcag gtcacgcctg acattgccgg
     3121 caatcgcgta ggcgacaacc agcggcggcg aggccaggaa gttcgcgcgg atgttcgggt
     3181 ggatacgggc ttcgaagttg cggttgcccg acagcacggc agcggcgacc acatcgttct
     3241 tcacgattgc ctcgttcagc gccggagtca ggtcgccggc gttgccgatg caggtcgtgc
     3301 agccgtaggc ggtcacgccg aagccgagct tttccagata gggcaggagg ccggcggctt
     3361 ccaggtactt ggtcaccacg cgcgatcccg gggccagcga agtcttgatg tgcggcgcga
     3421 ccttcaggcc ggcttccacc gccttcttgg ccaggaggcc ggcgcccagc agcacgctcg
     3481 ggttcgaggt gttggtgcac gaggtgatcg cggcgatcag cacgtcgccg ttatgcagct
     3541 tcacgccgtc ggccgtatcg tattcggcac cgaggtcctc ggccttcttg ttgaagccgt
     3601 tttccgagac cggcttggag aacagctcgg tgaaggtcga cttcacgttg ccgatttcga
     3661 tgcggtcctg cggacgcttc gggcctgcca gcgacggcgc gacgctgccc aggtccagtt
     3721 cgaccaccgc cgagtagtcg acctcgccct tcttcggaat gccgtacatc ttctgcgcct
     3781 tgaaataggc ttcgaaggcg tcgatttcgg ccttggtgcg gccggtgccg cggaagtaat
     3841 caatggtggc gtcgtcgacg gggaagaagc ccatcgtcgc gccgtattcc ggcgccatgt
     3901 tgccgatggt ggcacggtcg gtcagcgaca gcgactcggt gccttcgccg aagaactcga
     3961 cgaacttgcc gacgaccttg tgcttgcgca gcagttcggt gatcgtgagc accaggtcgg
     4021 ttgcggtcac gccttcgcgc agcgcgccgg tcaggtgcac gccgaccacg tccggggtca
     4081 ggaagtagac cggttggccc agcatgccgg cttccgcctc gatgccgccc acgccccagc
     4141 ccactacgcc gatgccgttg atcatcgtgg tgtgggagtc ggtgccgacc agggtgtcag
     4201 ggtagaacac gccttccttc ttgtgcacgc cgcgcgccag gtattcgagg ttgacctggt
     4261 ggacgatgcc gaagcccgga ggcacgacgc cgaaggtgtc gaaggcctgc atgccccatt
     4321 tcatgaactg gtagcgctcg ttattgcgct ggaattccag cttcatgttc aggtccagcg
     4381 ccttcttctc gcggaagtgg tcgatctgca ccgagtgatc gacgacgaga tccaccggca
     4441 ccagcggctc gatgttcttc ggcttcttgc ccatcttctg cgccacgccg cgcatcgcgg
     4501 cgaggtcggc caggagcggc acgccggtga agtcctgcag cacgacgcgc gcgaccacga
     4561 acgggatctc gtccacgcgc tgcgcattcg gcttccagtt cgccagctgg cgaacgtgtt
     4621 cttcggtcac cttcttgccg tcgcagttgc gcagcacgga ttcaagcacg atgcggatcg
     4681 acaccggcag gcgcgagatg ttgacgccga gcttcttctc gagtgccgga agcgagtaga
     4741 acttgccttt cctggaatcc gagatcttga attcctttag cgtgttcagg gtattgcggg
     4801 acatagcctc tccttgttta ttagctgaac gttgagtgcg ttgaaaatat ttttcttccg
     4861 atcctgctgc tatccgtgcc gggacgccgg tcagttcctg gcctgcttct tctgggtttc
     4921 gttgcggcag tcattcgcca cctgccggcc ctgcttcgag tccagcagca tgcccttggc
     4981 gggaatgccg atccagacca gcccgctgtc ggcgctttcg tagcgctcgg ctccggttga
     5041 ggtttccttg cgcgtcatct ggtgcgtgcg cttgttccac tgcagtgcga tctgctggtt
     5101 atcgtccgcc ttgcgataga tcgtgaggcg gccgagatcg cagtcatatg
//
LOCUS       DATLGF010000388         2258 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_183008, whole genome shotgun sequence.
ACCESSION   DATLGF010000388 DATLGF010000000
VERSION     DATLGF010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183008"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VM406_11890"
     CDS             complement(<1..417)
                     /locus_tag="VM406_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL76707.1"
                     /translation="MNTFLRKLDLTSLRLFVAVCREQNIARAAERELIAPSAVSRRIA
                     EIESVIGMPVIQRRSRGITVTPIGLTVLRHAEAVIASIEALAAELSQYSSGAMGEVRL
                     VANLSSIVQFLPEDIAAFQRVFPSVTIDVEEQNSVEA"
     gene            complement(424..1332)
                     /locus_tag="VM406_11895"
     CDS             complement(424..1332)
                     /locus_tag="VM406_11895"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HVL76708.1"
                     /translation="MRDLSRIARFFPFLRWPRPTLASLRADAWTGKLPAGFSAAFPVS
                     GSFSRSALNLYAGAVSAWSTLFAAACVLLSLLFLTGYLHYLLMAMLAAIIIVPVLNAS
                     DVAGLRRLFSVSRNDGVIALVTFAATMLALPDLYWGVAAGVGLNMVVFLYRRMHPRVI
                     EVGLHPDGTLRDRERFDLPRLAPDLIAVRMDSALIFLPRQCAGALRTRPLPRGPQHPP
                     RAVVRERLQRYRCQRSRFPAGAAPGAGRRRRGAVSERREDPGLERDGPVRPGRAARQA
                     ARLCDGSEGGGGARHPVLADWSRWSD"
     gene            complement(1551..>2258)
                     /locus_tag="VM406_11900"
     CDS             complement(1551..>2258)
                     /locus_tag="VM406_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HVL76709.1"
                     /translation="AFFGVLPGTGSTIASFMSYAVEKNVSDRPKEFGHGAIEGVAGPE
                     AVNNSAAHTAFIPSLTLGLPGDTVMALMLGAMMILGITPGPTLISEYPEVFWGLIASF
                     WVGNLMLIILNVPLIGLWVRLLTVPYRCIYQRCCSSCVGVYSYHNTYFDVALVLIFGI
                     LGFMFMRMGFEPAPLLLGSVLGPMLEEYLRRAPLLSRGDPMMLLERPLSATFLGIGAA
                     LVLWTLWSSFRGSARAK"
BASE COUNT          468 a          725 c          748 g          317 t
ORIGIN      
        1 agcctcaaca ctgttctgct cctcgacgtc gatggtgacg gatgggaaca cgcgctggaa
       61 tgcggcaatg tcctctggca ggaactgtac gatagacgac aggtttgcaa ccaggcgcac
      121 ctcgcccatc gcgcccgacg aatattgcga cagctcagcg gccagcgcct caatgctggc
      181 gatgacggct tcggcatgac gcagcacggt cagcccgatc ggcgtgaccg tgatgccgcg
      241 cgagcggcgc tggatcaccg gcatgccgat cacgctttcg atttccgcga tgcgccggct
      301 caccgccgac ggcgcgatca gttcgcgctc ggctgcgcgc gcgatgttct gctcgcggca
      361 tacggccacg aacagccgca gcgaagtcag gtcaagtttg cgcagaaatg tgttcattcg
      421 ggatcagtcg ctccatctgg accagtctgc gaggacagga tggcgagcgc ctccaccgcc
      481 ttccgatccg tcacaaagac gcgctgcttg ccgagccgct cgcccaggcc ggaccggtcc
      541 atcacgctcc agacctggat cttcacggcg ctcagataca gcgccacgcc ttcgccttcc
      601 agcgccaggc gcagcgcctg cagggaatcg agaccgctgg catcgatatc gttgcagccg
      661 ttcgcgcaca acagcacgcg gcggatgctg cggtcctcgc ggcagtggcc gagtacgaag
      721 cgctccagca cattgccggg gcaggaaaat gagcgccgaa tccatccgca ccgcgatcag
      781 gtcgggcgcc aggcgcggaa ggtcgaagcg ctcacggtcg cgcagtgtgc cgtccggatg
      841 caaaccgacc tcgatcacgc gcgggtgcat gcgccggtac aggaacacca ccatgttcag
      901 gccgacgccg gcggccacgc cccagtacag gtcgggcagc gcgagcatgg tggcggcgaa
      961 ggtgaccagc gcgatgacgc cgtcgttgcg cgacaccgag aacaggcggc gcaggccggc
     1021 cacgtcgctc gcattcagca ccggcacgat gatgatggcg gccagcatgg ccatcagcag
     1081 gtaatggagg tagccggtca ggaacagcag actcaagagc acgcaggcgg cggcgaacag
     1141 ggtcgaccag gcgctcactg cgccggcata taggttgagc gccgagcgcg agaacgagcc
     1201 gctgaccggg aaggcggcgg agaaaccggc ggggagcttg cccgtccagg cgtcggcgcg
     1261 cagcgacgct agggtcgggc gcggccagcg caggaaggga aagaaacggg caatccggga
     1321 caggtcgcgc atgaggtgaa acagcaaaat cgaagcgaac gctcgaactt atcacacgcg
     1381 cggtttgccg gcttgcacga tggaaatggg cacccggatc gtcataccgt tcagtgcctg
     1441 cctggaagac actgggcccc ggctttcgcc ggggtgacca tgaaacgatg ccgccttgac
     1501 tgaatggacc gcacccatcc ggggcctcag tgcggcccct gcgtgactac ctacttcgcc
     1561 cgtgcactgc cgcggaagct ggaccagagc gtccacaaca ccagggccgc gccgatgccc
     1621 aggaaggtcg ccgacagcgg acgctcgagc agcatcatcg gatcgccgcg cgagagcagc
     1681 ggcgcacggc gcagatattc ttccagcatc ggtcccagca cagagcccag cagcagcggc
     1741 gccggttcga agcccatgcg catgaacatg aagccgagga tgccgaagat cagcaccagc
     1801 gccacgtcga agtaggtgtt gtggtacgag tacacgccca cgcatgaaga acagcaccgt
     1861 tgatagatgc agcgataggg cacggtcagc aggcgcaccc agagcccgat cagcggcacg
     1921 ttcaggatta tcagcatcag gttgccgacc cagaacgagg cgatcaggcc ccagaacact
     1981 tccgggtact cgctgatcag ggtggggccg ggggtgatgc cgagaatcat catcgcgccg
     2041 agcatcagcg ccatcacggt gtcccccggc aggccgagcg tgagggacgg gatgaaggcg
     2101 gtatgggcgg cggagttgtt gacggcctcg ggaccggcga caccctcaat cgcgccatgg
     2161 ccgaactcct tcggccggtc cgagacattc ttctcgaccg cataggacat gaacgaagcg
     2221 atggtggaac cggtacccgg cagtacgccg aagaaggc
//
LOCUS       DATLGF010000389         2662 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_152270, whole genome shotgun sequence.
ACCESSION   DATLGF010000389 DATLGF010000000
VERSION     DATLGF010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152270"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VM406_11905"
     CDS             <1..377
                     /locus_tag="VM406_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76710.1"
                     /translation="TEVPALGPLGPIAGGLLGGHLGGLIGGLASMRESGDVGPEDEDP
                     ENAMPMRHAGMYVAVVVPPDHGDGERMAADALIALGATDLELSEGNIVNGDWVDFDPS
                     IPPHPYQRVPRESRPSAPTQRA"
     gene            complement(462..1190)
                     /locus_tag="VM406_11910"
     CDS             complement(462..1190)
                     /locus_tag="VM406_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HVL76711.1"
                     /translation="MAAVPEPSAAPQRAPWPLTVATYNIHAAVGRDGRYAPDRIIDVL
                     HELQADIVALQEVPLGGTQCDNVLTMLTEATGYESAEGPACDTVERRYGNAVLSRFPI
                     LSVRAIDLSFGSREPRGALDADVDCGGDLLRVVATHLGLRPAERRHQIRRLLDVFDTD
                     EMPVILMGDINEWFVWGKSLRMLVSHFQAVPAPATFPSGWPVFALDRIWIRPRHRLVH
                     VEVHATPLAQVASDHLPLIAHIDG"
     gene            complement(1190..>2662)
                     /locus_tag="VM406_11915"
     CDS             complement(1190..>2662)
                     /locus_tag="VM406_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HVL76712.1"
                     /translation="THTRLWPGIEPDLRDVDVAISRTEPEFDGRPAVHEVRQLHLDAI
                     TAAQRHLFFENQYFTSGMIANALAARLERAEGPEVCIVSPRTQSGWLEQATMGLLRAR
                     VHRRLRQADRHRRYQMLCPQVPGLGEDCLNVHSKVFTVDEALLSIGSANLSSRSMALD
                     TECNLSIEAHGEVRIAAAIAHMRNRLLAEHLDTTPQAVQSEIERRGSLLAAIAALSHG
                     ERRLAPMEPEVVPELDALIPEQAVFDPEKPIKPDELVAQLVPREARKPAPRRLIGLGM
                     LALALALLAVAWRWTPLRDVVNLGSLVALARSLEALPFTPLAVMASYVVAGLLMVPVM
                     LLIAVTGIVFGPFEGSLYAAGGCVLSAAAGYGLGRWLGRETVQRMLGPRMNRLSRRLA
                     RQGIAAMIVIRMLPIAPFTVVNIVAGTSHIRLRDYLIGTLLGMLPGIFVTVTFVHHLA
                     EAIRNPSVGTVAVLGTVALALIGLAMGLQKLLRRKDDAAV"
BASE COUNT          431 a          908 c          928 g          395 t
ORIGIN      
        1 ccaccgaggt gcccgccttg gggcccctcg ggccgattgc cggggggctg ctcggcgggc
       61 acctcggtgg cctgatcggc ggccttgcct cgatgcggga atcgggcgac gtgggcccgg
      121 aggacgaaga tccggaaaac gccatgccga tgcgccatgc cggcatgtac gtggccgtgg
      181 tggtaccgcc ggaccacggg gatggcgaac ggatggccgc cgatgccttg atcgcgttgg
      241 gggcgactga cttggaatta agcgagggca acattgtcaa tggcgactgg gtggatttcg
      301 atccttcgat accgccacac ccgtaccagc gcgtgccccg ggaaagccgg ccgagcgcgc
      361 cgacgcagcg ggcctgacga ggtccacagc ccgcctggac tgcaggccgg gctgacacca
      421 gggaatcgcc atgtccccca agcggttcat gcgggggcgt ctcagccgtc gatgtgcgcg
      481 atcagcggca gatgatccga cgccacctgc gccagcggcg tagcgtgcac ctcgacatgc
      541 acaaggcggt gccgcggtcg gatccagatc cggtccagcg cgaacaccgg ccaacctgaa
      601 ggaaaggtcg ccggcgcggg cacggcctga aagtgcgaca ccagcatgcg cagtgacttt
      661 ccccatacga accattcatt gatgtcgccc atcaggatga cgggcatctc gtcggtgtcg
      721 aagacgtcga gcaggcgccg gatctgatgg cgccgctcgg ccgggcgcag gccgaggtgg
      781 gtggcgacca cgcgcagcag gtcgcccccg cagtccacat ccgcatcgag cgcgccgcgc
      841 ggctcacggc tgccgaacga caggtcgatc gcgcgcaccg acaggatcgg aaagcgcgac
      901 agcaccgcgt tgccgtagcg ccgttccacg gtgtcgcagg ccggcccttc cgcgctttcg
      961 tagccggtcg cctcggtcag catcgtcagc acgttgtcgc actgggtacc gccgagcggc
     1021 acttcctgca gggccacgat atcggcctgg agttcatgca gcacgtcgat gatgcggtcc
     1081 ggcgcatagc ggccgtcacg gccgacggca gcgtggatgt tgtacgtggc cacggtcagc
     1141 ggccagggtg cgcgctgggg ggcggcggaa ggttcgggta cggccgccat cacacggccg
     1201 cgtcgtcctt gcgccgcagc agcttctgca ggcccatggc gagcccgatc agcgcgagcg
     1261 ccacggtacc gagcaccgcg acggtgccga cgctgggatt gcgtattgct tccgcgaggt
     1321 gatgcacgaa ggtgacggtc acgaagatgc cgggcagcat gcccagcagc gtgccgatca
     1381 ggtagtcgcg cagccggata tgcgaggtgc cggcgacgat gttgacaacg gtgaacggtg
     1441 cgatcggcag catgcggatc acgatcatcg ccgcgatgcc ctgccgggcc agccttctcg
     1501 agagccggtt catgcgcggg ccgagcatgc gctgcacggt ctcgcgtccc agccagcgcc
     1561 cgaggccata gccggccgcc gcgctcagca cgcagccgcc ggccgcgtac agcgatccct
     1621 cgaacggccc gaacacgatg ccggtcacag cgatcagcag catgaccggg accatcagca
     1681 gccctgcgac cacatagctg gccatcaccg cgagcggcgt gaacggcagc gcttccaggc
     1741 tgcgggccag cgccaccagc gagccaaggt tgaccacgtc gcgcagcggc gtccagcgcc
     1801 aggcgactgc cagcagagcc agcgcgagcg cgagcatgcc cagtccgatc agccggcgcg
     1861 gcgccggctt gcgcgcctcg cgcggcacca gctgggcgac cagctcatcc ggcttgatcg
     1921 gtttttccgg gtcgaacacc gcctgctccg gaatcagcgc gtcgagctcg ggcacgacct
     1981 ccggctccat cggcgccagc ctgcgttcgc cgtgcgacag cgcggctatc gccgcaagca
     2041 ggctgccgcg ccgctcgatc tcactctgca ccgcctgcgg cgtggtatcc agatgctcgg
     2101 cgagcaggcg gttgcgcatg tgcgcaatcg ccgcggcaat gcgcacctcg ccatgggctt
     2161 cgatgctgag gttgcattcg gtgtccagcg ccatcgagcg gctcgacagg tttgccgacc
     2221 cgatactgag caaggcttcg tcgactgtga ataccttgct gtgcacgttc aggcagtcct
     2281 cgcccaggcc gggtacctgc ggacacagca tctggtagcg ccggtgccga tcggcctgcc
     2341 gcaggcgccg atgcacgcgc gcgcgcaaca ggcccatcgt cgcctgctcg agccagccgc
     2401 tttgggtgcg gggcgacacg atgcacactt ccggaccttc ggcgcgctcc aggcgcgcgg
     2461 cgagcgcgtt ggcgatcatg ccggaggtga agtactggtt ctcgaagaac agatggcgtt
     2521 gcgcggccgt gatcgcgtcc agatggagtt gccggacctc gtgcaccgcg gggcggccgt
     2581 cgaattccgg ctcggtgcgc gagatcgcca cgtccacgtc gcgcaggtcc ggttcgatgc
     2641 ccggccacag gcgggtgtgc gt
//
LOCUS       DATLGF010000390         3015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_172049, whole genome shotgun sequence.
ACCESSION   DATLGF010000390 DATLGF010000000
VERSION     DATLGF010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172049"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VM406_11920"
     CDS             complement(<1..340)
                     /locus_tag="VM406_11920"
                     /inference="COORDINATES: protein motif:HMM:NF015666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76713.1"
                     /translation="MAAPLIRRHQANAPVTSSEDRTSPAAVVISTLPPSGGTEQLSSV
                     TSSYGEASRNNKGAVMRISVFGLGYVGTVSAACLARQGNKVIGVDANPIKVDMINRGL
                     SPIVEKDLGGL"
     gene            complement(477..2501)
                     /locus_tag="VM406_11925"
     CDS             complement(477..2501)
                     /locus_tag="VM406_11925"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HVL76714.1"
                     /translation="MPSLGWMFNRLRLMGPAEVAWRSRKALAARFERARAAIAPPTPP
                     APTARDVFGKPWLGGIPDFPAFDAAPYCAAAERILAGRYDVFALRDLALGFPPEWNRD
                     PKTGVLAPLTFGKTLDYRDERTVGDIKYLWEINRHYEIVTLAQAWRLSEDDRFAEGCR
                     RMIDSWIEDCPYPLGANWSSALEHALRLVNWAAAWQLLGGAEAPLFQGAQGAAFRQRW
                     LAAVHRHCDFIASHLSKYSSANNHLFGEYMGMFIASLTWPCWAESSRWRDTARQGLEA
                     EALKQIADDGVNLEQAIWYHHEVTEMMLLCTLSARRNDVHFARAWWARLEAMCEFIAA
                     VMDVDGNVPMIGDADDAVMLRLAPDRTQNVYRSLLATAAVLFSRPDFARKAGTFDDKS
                     RWLLGQDAAFDFAALKADGARQPRGSGEPPPRLAFPHGGYYVLGSALDTPEEVRIVAD
                     AGPLGYLSIAAHGHADALSFTLSAAGREILIDPGTCAYHTQPAWRAYFRGTSAHNTVR
                     IDGRDQSEAGGNFMWLHKAHAQCELWDSDEDADRFAAWHDGYQRLPDPVIHRRKLLYM
                     KSERVLVVQDSFECASSHGLEFWWHFAEDCIVNVHGGMIEARCGPVRMTMERDTPPES
                     LELVSGAEQPQAGWISRRFDERHPTTSVVLRERISGSASRITTLRIDIEQ"
     gene            complement(2489..>3015)
                     /locus_tag="VM406_11930"
     CDS             complement(2489..>3015)
                     /locus_tag="VM406_11930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVL76715.1"
                     /translation="GDRKGTYDLLRAVARVVKTHPDLKLVLAGDGEIERVRREVVRLG
                     LTSHVEVLDWVSGRRKEALLERAAIYVLPSYCEGLPMSVLEAMAAGLPVVTTPVGGVP
                     EAITDGLEGRLVVPGDVDALCEALDALLSQLELRRQMGQAARAKIETTFSATCVLPQL
                     EQLYRQLGIRPCLR"
BASE COUNT          533 a         1006 c          978 g          498 t
ORIGIN      
        1 tcaatccacc caggtccttt tcaacgatcg gagagagtcc gcggttgatc atgtcaacct
       61 tgatcggatt cgcgtccacg ccgatcacct tgttgccttg gcgcgcaagg cacgccgccg
      121 aaaccgtgcc cacatagccc agcccgaaaa cactgatgcg catgacagct cctttgttgt
      181 tgcgagacgc ctcgccatac gacgaggtga ccgatgagag ttgttccgtg ccgccggatg
      241 gcggcagggt ggagatgaca acggctgcgg gcgacgtgcg atcctcgctt gatgtaaccg
      301 gagcgtttgc ttggtgtcgc ctgatcagag gggcagccat gtgatgaatc aagcatgtaa
      361 caggaggtgt ctagtgaacc agacgcgtgt cgccccgggt ttgacggtgc gcagagtttg
      421 cagttctcgg gattgggggc agatcagaac cgcgccaagt tggggcgcga cgccaactac
      481 tgttcaatat ctatacgcag tgtcgtgatg cgcgacgcgc tgccggagat gcgctcgcgc
      541 agaaccaccg aggtagtcgg gtgacgttcg tcaaagcggc gcgaaatcca gccggcctgc
      601 ggttgctccg cgcccgacac gagctccaat gactccggcg gcgtgtcgcg ttccatcgtc
      661 atgcgaacag ggccgcagcg cgcttcgatc atcccgccgt gtacgttgac gatacagtct
      721 tcggcaaagt gccaccagaa ctcgagcccg tgcgaggacg cgcactcgaa actgtcctgc
      781 accacgagta cgcgctcact cttcatatag aggagcttgc gacgatggat gaccggatcc
      841 ggcagtcgtt ggtagccatc gtgccaggct gcgaagcggt cggcatcctc atcgctgtcc
      901 catagctcgc actgcgcatg cgccttgtgc agccacatga aattgccgcc cgcctcggac
      961 tggtcgcgac cgtcgatgcg caccgtgttg tgcgcggagg tgccgcgaaa gtacgcgcgc
     1021 caggccggct gggtgtggta cgcgcaggtg ccgggatcga tcaggatctc ccggccggcg
     1081 gctgacagcg tgaacgacag cgcgtcggcg tgcccatgcg cggcgatcga caggtagccc
     1141 agcggtccgg cgtcggcgac gatgcgcacc tcctccgggg tatcgagcgc ggacccaagc
     1201 acgtagtagc cgccatgcgg aaaggcaagg cggggcggcg gctcgccgct gccccgcggc
     1261 tggcgggcgc catcggcctt gagcgcggcg aagtcgaacg ccgcgtcctg cccgagcagc
     1321 cagcgcgact tgtcgtcgaa agtgccggcc ttgcgcgcaa aatccgggcg cgagaacagc
     1381 acggcggcgg tcgccagcag cgagcggtag acgttttgcg tgcggtccgg cgccagccgc
     1441 agcatcaccg cgtcgtccgc gtcgccgatc atcgggacgt tgccgtccac atccatcacg
     1501 gccgcgatga attcacacat cgcttccagc cgcgcccacc acgcgcgcgc aaagtgcaca
     1561 tcgttgcggc gcgcgctcaa cgtgcatagc agcatcattt ccgtcacttc gtggtgatac
     1621 cagatcgcct gctccaggtt gacgccgtcg tccgcaatct gcttgagcgc ctcggcttcc
     1681 agcccctgcc gcgcggtgtc gcgccagcgg ctgctctcgg cccagcacgg ccaggtcagc
     1741 gatgcgatga acatgcccat gtattcgccg aacagatgat tgttcgccga tgaatacttc
     1801 gagagatggc tggcgatgaa atcgcaatgc cggtgcacgg cggcgagcca gcgctggcgg
     1861 aacgccgctc cctgcgcgcc ctgaaacagc ggcgcctcgg cccctccgag caattgccag
     1921 gccgcagccc agttcaccag gcgcagcgca tgctcgaggg cgctcgacca gttcgctccg
     1981 agcggatacg ggcagtcctc gatccaggag tcgatcatgc gccggcagcc ttcggcaaaa
     2041 cggtcgtcct cggacagccg ccaggcctgc gccagcgtga cgatttcgta gtggcgattg
     2101 atttcccaga ggtacttgat gtcgccgacc gtgcgctcgt cgcgataatc gagcgtcttg
     2161 ccgaaggtca gcggcgcaag cacgccggtt ttcggatcgc ggttccattc gggaggaaaa
     2221 ccgagcgcga ggtcgcgcag cgcgaatacg tcatagcggc cggcgagaat gcgctcggcg
     2281 gcggcgcagt atggcgccgc gtcgaacgcc gggaaatcag gaatgccgcc cagccagggc
     2341 ttgccgaaca cgtcgcgcgc ggttggagcg ggcggggtcg gaggcgcaat cgccgcgcgc
     2401 gcacgctcga agcgggcggc cagcgccttg cggctgcgcc atgcgacctc cgccggcccc
     2461 atcaggcgca ggcggttgaa catccaccct agcgaaggca tgggcggatc ccgagctgcc
     2521 tgtacagttg ttcgagctgc ggcagtacgc aggtcgccga gaacgtggtt tcgatcttgg
     2581 cgcgggccgc ctgccccatc tggcggcgca gctcgagctg cgacagcagg gcgtcgagcg
     2641 cctcgcacag cgcatcgaca tcgcccggca ccaccagccg tccctccagc ccgtcggtga
     2701 tcgcctcggg gacgccgccc accggcgtgg ttaccaccgg caacccggca gccatcgctt
     2761 cgagcacgct catcggcaac ccttcgcaat aggatggcag cacgtagatc gcggcacgtt
     2821 cgagcagcgc ttccttgcgc cggccgctga cccagtccag cacttccaca tgggaagtca
     2881 gaccgaggcg cacgacttcc cggcgcacac gctcgatctc gccgtcgccc gcaagaacca
     2941 gcttcagatc gggatgcgtc ttgaccacgc gcgccaccgc tcgcagcagg tcataggtgc
     3001 ccttgcgatc tccaa
//
LOCUS       DATLGF010000391         3509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_94373,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000391 DATLGF010000000
VERSION     DATLGF010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94373"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..990
                     /gene="hutC"
                     /locus_tag="VM406_11935"
     CDS             259..990
                     /gene="hutC"
                     /locus_tag="VM406_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine utilization repressor"
                     /protein_id="HVL76716.1"
                     /translation="MAVSDKQRRKQGEAVPAYRHIKDYLLAQVHAGTWQEGDAIPSEQ
                     ELAREFGVSRMTVNRAVRELTDEQVLLRIQGSGTYVAQQKYQSTLVEIKSIADEVRAR
                     GHQHRSELHLLERRKVGEGMARAFELKAGAPLFHSLIVHFENETPIQVEDRWVNPQAA
                     PDYMQQDFSVVTPNEYLMRAAPLQGVQYSIEALPAPADIAEHLSIAPDSPCLVLHRRT
                     LSMGRVASVATMWHPGACYRFAGSF"
     gene            1298..1555
                     /locus_tag="VM406_11940"
     CDS             1298..1555
                     /locus_tag="VM406_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HVL76717.1"
                     /translation="MLDDKAAAYWRATLRLLAITLTIWFIVSFVAGILLVDVLDGISI
                     GGYPLGFWFAQQGSIYVFVVLIFWYARRMGKIDREHDVHEQ"
     gene            1572..3353
                     /locus_tag="VM406_11945"
     CDS             1572..3353
                     /locus_tag="VM406_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HVL76718.1"
                     /translation="MSLQALTYLVVGLSFALYIGVAIWARAGSTRDFYVAGGGVHPVV
                     NGMATGADWMSAASFISMAGLIAFLGYDGSVYLMGWTGGYVLLAVLLAPYLRKFGKFT
                     VPQFIGDRYYSQGARMVAVICLIFISFTYVAGQMRGVGIVFSRFLEVEIGTGLVIGMG
                     LVFFYAVLGGMKGITYTQVAQYVVLITAYTIPAIFISLQITGNPLPQLGLGSTVADGT
                     HFLAKVDQVVTDLGFAEYTVRSRPMIDIFAITFALMAGTAGLPHVIVRFFTVPKVADA
                     RSSAGWALVFIAILYTVAPAVGAMARYNLHATISPGAIAGQPFAPEANIQFEERPDWM
                     RRWERTGLLKFQDLNNDGRIQYYNDKSKDPEFQKRVAEAGWQGNELTVDRDIMVLANP
                     EVAQLPNWVIALVAAGGIAAALSTAAGLLLVISAAISHDLMKSMFTPNLSERGELLAG
                     RISAAVAVLIAGYLGFNPPGFVAQVVAFAFGLAAASLFPTIMLGIFSKRMNKEGAIAG
                     MLSGLIFTLVYIVYFKRDVLFGASPVPDSQWLFGISPEGIGMIGALINIVVAFVVLKL
                     TRPSPEHIQHLVEDIRTPRGAGLAQGH"
BASE COUNT          604 a         1089 c         1134 g          682 t
ORIGIN      
        1 gttctggatc atccggtacg cggcctcggt cagccagttc cggcaggaga gcgttgagcc
       61 gcgcggcgcg cgaatcttgc ggtcggaatc gaaacgggga tcggagcggg gatcggtggc
      121 gggagcagcc atagcagttc cttgatcggc ggtggaatgc gagcagttta agttgtatat
      181 acaacttatg caaggcgaag acgagttggc tggacaggtt aaactgctgc aatcggttac
      241 acgcggggag aaagcggctt ggcagtctcg gataaacagc gacggaaaca gggcgaggcg
      301 gttccggcct atcggcatat caaggattac ctgctggcac aggtccatgc cggcacctgg
      361 caggagggcg acgcgattcc ctcggaacag gagctggcgc gcgagttcgg cgtgtcgcgg
      421 atgacggtga accgcgcggt gcgcgaactc accgacgagc aggtcctgct gcgcatccag
      481 gggtccggca cctacgtcgc ccagcagaaa taccagtcga cgctggtcga gatcaagagc
      541 attgccgacg aggtgcgcgc acgcgggcat cagcaccgca gcgagctcca tctgctcgag
      601 cgccgcaagg tgggggaggg gatggcgcgc gcgttcgagc tcaaggccgg cgccccgctg
      661 ttccattcac tgatcgtcca cttcgagaac gagacgccga tccaggtcga ggaccgctgg
      721 gtgaatccgc aagccgcgcc ggactacatg cagcaggatt tctccgtcgt cacacccaac
      781 gaatacctga tgcgcgccgc gccgctgcaa ggcgtgcagt acagcatcga ggcgctgccc
      841 gcgccggccg acattgccga gcatctgagc attgcccctg actcgccttg cctggtcctg
      901 caccggcgca cgctgtcgat ggggcgcgtc gcatcggtcg cgacgatgtg gcatcccggc
      961 gcatgctatc gcttcgccgg cagtttctga gatctcgcgg caccccggtg ttattcgagt
     1021 gaaacctgcg gggcgctgct ccggcttgcg tcgcgacggc accggaaccc cggaagcaaa
     1081 cagcagcacc cgcgtcttca catgcagctt gatgtctccc caatcgtgct gccgcacaga
     1141 gcgcgtgcat ttgatattcg tcatccccgg catcgtgtat ccgcgtttcc ctacatgcgt
     1201 gaagcaggtt tcggtcgcgg taagggcgct gtaagtttgt ggcgctatgc tcgttccata
     1261 ctgcttccat ccaatactca cgatcaagga gacggacatg ttggacgaca aggcggccgc
     1321 ctattggcgc gcgacactga ggttgctggc gatcacgctg accatctggt tcatcgtttc
     1381 attcgtggca ggcattctgc tcgtcgacgt gctggatgga atttccatcg gcggttatcc
     1441 gctcggattc tggtttgccc agcagggatc gatctacgta ttcgttgtcc tgatcttctg
     1501 gtacgcgcgg cgcatgggca agatcgatcg cgagcacgac gtccacgaac aataagaacg
     1561 ggagagcgga catgtcacta caggcactca cttatctcgt cgtcggcctg agcttcgcgc
     1621 tctacatcgg cgtcgcaatc tgggcgcgtg cgggcagcac gagggatttc tacgtggccg
     1681 gaggcggtgt gcatccggtc gtcaacggca tggcaaccgg cgccgactgg atgtcggcgg
     1741 cctccttcat ctcgatggcc ggcctgatcg ccttcctcgg ctatgacggc tccgtgtatc
     1801 tgatgggctg gaccggcggc tacgtgctgc ttgccgtgct gctcgcgccg tatctgcgca
     1861 agttcggcaa gttcaccgtg ccgcagttca tcggcgaccg ctactactcc cagggcgcgc
     1921 gcatggtcgc ggtcatctgc ctcatcttca tctccttcac ctacgtggcc ggacagatgc
     1981 gcggcgtcgg catcgtgttc tcgcgctttc tcgaagtgga aatcggcacc ggcctggtga
     2041 tcggaatggg gctggtgttc ttctatgcgg tgctgggtgg aatgaaggga atcacctata
     2101 cccaggtggc ccagtacgtg gtgctgatca ccgcatacac gatcccggca atcttcatct
     2161 cgctgcagat caccggcaat ccgcttccgc agctcggcct gggcagcacg gtcgccgacg
     2221 gcacacactt cctcgcgaag gtcgaccagg tcgtcaccga tctgggattt gccgagtaca
     2281 cggtgcgcag ccgcccgatg atcgacatct tcgcgatcac cttcgcgctg atggcgggca
     2341 cagccggatt gccgcacgtg atcgtgcgct tcttcacggt gccaaaggtg gccgatgcgc
     2401 gcagctcggc cggctgggcg ctggtcttca tcgcgatcct gtacacggtc gcgccggcgg
     2461 tgggcgcaat ggcacgctac aacctgcacg cgacgatctc gccgggcgcg atcgccgggc
     2521 agccgttcgc gcccgaggcc aacatccagt tcgaggaacg tccggactgg atgcgccgct
     2581 gggagcgcac cggcctgctc aagttccagg acctgaacaa cgacggccgc atccagtact
     2641 acaacgacaa gtcgaaggac ccggaattcc agaagcgcgt ggccgaggcc ggctggcagg
     2701 gcaatgagct gacggtggac cgcgacatca tggtgctcgc caacccggaa gtcgcgcagc
     2761 tgccgaactg ggtgatcgcg ctggtcgcgg ccggggggat cgccgctgcg ctctcgacag
     2821 cggccgggct tctgctggtg atctcggccg cgatctcgca tgacctgatg aagagcatgt
     2881 tcacgccgaa tctatccgag cggggcgaat tgcttgcggg gcgaatatcg gccgcggtcg
     2941 cggtgctgat cgccggctat ctcggcttca atccaccggg cttcgttgcg caggtggtgg
     3001 cgtttgcgtt cggattggcg gcggcctcat tgttcccgac catcatgctc ggcatcttca
     3061 gcaagcggat gaacaaggaa ggcgcgatcg ccggcatgct gagcggtctg atcttcacgc
     3121 tcgtctacat cgtgtacttc aagcgcgacg tgctgttcgg cgcaagcccg gtaccggaca
     3181 gccagtggtt gttcggcatc tcgccggaag gcatcggcat gatcggtgcg ctgatcaata
     3241 tcgtggtcgc cttcgtggtg ctcaagctca cgcgtccttc gccggagcac atccagcacc
     3301 tcgtggaaga catccgcacg ccgcgcggtg ccgggcttgc ccagggccac tgacgcaatc
     3361 gtcgaagccg ggcgcgccgc cgacgcggtg cgcccgtttc gtctgcatga gcacgctgca
     3421 tgatctatct ggaactcgcc gtcgcctggc tgctcttcct cgcgatcccc gcgctcgcgg
     3481 gcggcatgct gtatcgggcg tggaagatc
//
LOCUS       DATLGF010000392         8310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_75129,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000392 DATLGF010000000
VERSION     DATLGF010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 8310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8310
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75129"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            378..1094
                     /locus_tag="VM406_11950"
     CDS             378..1094
                     /locus_tag="VM406_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76719.1"
                     /translation="MRSDFIPQFASDVDEAHEKQRKEAMRTIEQHGMRPMSDLADMSE
                     GERVKWFFWNLHENLEEIRKLEPTLIGQVMCTTLTVTDGLSMDTERVGMEKSIALSCR
                     WHLRLAYSTFQNEEAYPIGDGAVDLIVSNALPQEPPLKKNQKGYLDADSSLYPHQLYL
                     YGWVTEQIWNEIKPHLYSPTPNCQTDLILRDNCMFPVKQGFAFVAGPPGAVGLTNVEF
                     RVASHSAERRTSRRAETLQR"
     gene            1233..1784
                     /locus_tag="VM406_11955"
     CDS             1233..1784
                     /locus_tag="VM406_11955"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HVL76720.1"
                     /translation="MFPSQQQIAAAARAQFDTQVALYREFSQTALDSMEKLTRLNIAA
                     VRASMEENSALARQMLTARDMQQAVSVMRTQTGPSIGKAIAYGNHVVNIASDAQADLT
                     RATEVQLAEAARRAGELVDEAARNAPPGTENLMALVKVAIGSATAGYEEVNRSTRRAV
                     QTLESNVNAAVNQIVQPAPTTGG"
     gene            complement(1910..2212)
                     /locus_tag="VM406_11960"
     CDS             complement(1910..2212)
                     /locus_tag="VM406_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76721.1"
                     /translation="MHATDTAATPDHAEALASLRASLDALAQRRIATDAFCRIWRAQH
                     ALLACLPARYGLVMEDLLGRMESGSLFGEESCSFSQEELRASLATWLDKAAQSLGA"
     gene            complement(2221..2592)
                     /locus_tag="VM406_11965"
     CDS             complement(2221..2592)
                     /locus_tag="VM406_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HVL76722.1"
                     /translation="MTYYTLKHLHVACALVSGLLFGMRGLWMLQASPLAQRRSLRLAA
                     HVIDTVLLVSAIGLVVWSARYPLLQDWLTVKVIAVFAYIGVGSIALKRGSSRFVRGVA
                     FVTALAIFAYILKLALTKQPF"
     gene            complement(2600..2998)
                     /locus_tag="VM406_11970"
     CDS             complement(2600..2998)
                     /locus_tag="VM406_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HVL76723.1"
                     /translation="MHQGSCLCGAIRYEITAPIKRITHCHCSMCRKAHGAAFGSYVTV
                     AHGAFRFTEGQDMVAAYSSSPGVTRTFCKRCGSTLQWYSTIHHPETVSIAAGTLDTSP
                     PPPPQQHIYAAFKASWYRIEDAIPQAEAGL"
     gene            complement(3072..3509)
                     /locus_tag="VM406_11975"
     CDS             complement(3072..3509)
                     /locus_tag="VM406_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76724.1"
                     /translation="MPSAPSFRRTRSLPAGLLTLAALGVAPHAPAYAQAGASSVPARL
                     AAPVLVRYVYYPDLQVYYSTQQQIWFWMNGSGWSYGVDLPDTYRERLGTGIALKLATA
                     RPFEEHASVEQRIGRPWRRQQELRREEQGGDAQARGAADKDNG"
     gene            complement(3658..4080)
                     /locus_tag="VM406_11980"
     CDS             complement(3658..4080)
                     /locus_tag="VM406_11980"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HVL76725.1"
                     /translation="MQTRMTVDHFNRVGADCLPGQFGIVVTEIGDKTLQAHLAVRPQL
                     LAPNGYLHAGTIVTLADTACGYACMAHLPEGAVSFTTIELKSNFVGTAREGTIDCTAQ
                     AVHTGRNTQVWDALVRHRETDKLIATFRCTQMVLWPKS"
     gene            4222..5313
                     /locus_tag="VM406_11985"
     CDS             4222..5313
                     /locus_tag="VM406_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL76726.1"
                     /translation="MNVLSKHETEALARHVAEGAGAPGAGMRTQTALIAAGAALAATA
                     LVVQRRSQDVERTHPPIGKFIEVDGVRLHYTERGDGQPLVMLHGVGAMMQDLIISGIV
                     DMAASKYRVILFDRPGYGYSERPRTTIWTPHAQARLLHKALQQMGIAQPIVFAHSWAG
                     MVATAMALDFPDDVKSLVLLSGYYYPTARLDIPYMAAPSIPLVGDLMRYTISPLLGRA
                     IWPAMKRKLFAPAPVPRRFEQEFPTWMTLRPSQLRASAAEYALLIPAAYAMQARYHEL
                     LLPVVIMAGDGDRHVDTRLHSERLHHELPHSSFLVAHGAGHMVHHCAQDQVMAAIDEA
                     ATAVGAVPVEHQRAQAGQSYVFPSPGQVS"
     gene            complement(5306..6223)
                     /locus_tag="VM406_11990"
     CDS             complement(5306..6223)
                     /locus_tag="VM406_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVL76727.1"
                     /translation="MSDPVLHKPAPASPNGAMPHAVFRTLHGTNAELERFAWLGDELA
                     VAIWKRQTDAAETAYRQPGHHTLSFYLGGGYRTQRSELPGQYGAPRMLCTLPDDHDSR
                     WLIRGDLRFLHLYFMPRHFTRRAVLELDREPREVTLADRTYFANPTIERLCERLAAMP
                     WDGADAQLRANETAHAALSQLLLSQSAPRRGGERLRGGLAPAVRRRLSDYIEAHLHLP
                     ITLGMLAELACLSEYHLARMFRVSFGMPPHAWIAARRIDRARQLLGAANLPLQQVADA
                     CGYADLSHFSHRFRAAVGTAPGRYRWLFS"
     gene            6419..7309
                     /locus_tag="VM406_11995"
     CDS             6419..7309
                     /locus_tag="VM406_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HVL76728.1"
                     /translation="MNASLYILTVLIWGSTWIAIKWQLGVVPAPVSIAWRFWIAGAVL
                     LALLAILRKPMRPPRAAFPYLAAQGVALFCCNFLCFYYASQWVASGLVAVVFSTATLW
                     NAINGRIFLGRALQPQVLAGAGMGIVGIVLLFLPQMSGHWTDAGVLPGLGLALLGTLC
                     FSTGNMLSARMQAMGLTPWMTNTWAMFIGAGVLTVIALVLGMPFALDTSARYLGSLLY
                     LAIPGSVIGFTAYLMLVGRMGPERAAYCTVLFPVVALALSTLFEGYVWDVWAVAGLVL
                     VLCGNLLAFMPLRWLRPSPA"
     gene            complement(7306..7920)
                     /locus_tag="VM406_12000"
     CDS             complement(7306..7920)
                     /locus_tag="VM406_12000"
                     /inference="COORDINATES: protein motif:HMM:NF027851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HVL76729.1"
                     /translation="MSYILDALKRAEAERIAGVAAPAPLPSAYAAPPATRRLAHRFAL
                     PLGAALLAGLIGAAAFVLRADPTTEAPPAEVLPAVAEPQAPAASAEASIIPPPSGPSP
                     IVRAPAPPVRAAAPARSPAPPAQTVSARPGTMRDLPEAIQREIPPLVIGGYLYAANPA
                     DRTVLINQRLRREGDEIAPGLVLESLQPDGMVLNYRGHRYRSTY"
     gene            complement(7920..>8310)
                     /locus_tag="VM406_12005"
     CDS             complement(7920..>8310)
                     /locus_tag="VM406_12005"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HVL76730.1"
                     /translation="YHALLVHAQGGRYRLQAAGAHQEVGLADLTRHFRGEFVTLWQTP
                     PGLGATIALGQQGAAVDWVGTQLARVHGTELPPARQPFNLELHRQVRLFQQSQGLAVD
                     GVVGPVTLMHLNRAAGVAEPRLNEAGS"
BASE COUNT         1394 a         2652 c         2839 g         1425 t
ORIGIN      
        1 cctttgtgac cggtgcgaac atttcgatca atggcggtca gcacatgcaa tgagaaggac
       61 ggcgctgcgc gccggctgct gttcctctcg cgcccgcttc gtgcgggcgt ttttttcgcc
      121 ggcgaaaagc cgcccggcgt gccggaaatc cggtgggcgg gcgctttctt gatgattgtc
      181 attgaacggg aagtgttcgg gattcgatac tacccatgcg ccgcgatgaa cggccgaatg
      241 tcgacgcgcg ccccgggtaa tcctgcgcga tgcccaattc tgtcgtggcg gcatgaactt
      301 agtacagcag cagcggctca atagcatacg catgtgcgcc acgcgttttt tcaatccgcc
      361 taatctggag atgcccaatg cggagcgatt tcatcccgca atttgcaagt gatgttgacg
      421 aagctcacga aaagcagcgc aaggaagcga tgcgcacgat cgagcagcat ggcatgcggc
      481 cgatgtcgga tctggcggac atgagcgagg gcgagcgggt caagtggttc ttctggaacc
      541 tgcatgaaaa cctcgaggaa atccgcaagc tggagccgac cctgatcggg caggtgatgt
      601 gcaccacgct caccgtcacc gacgggctgt cgatggacac cgaacgtgtc ggcatggaga
      661 agagcattgc gctgtcctgc cgctggcacc tgcgcctggc gtacagcacc ttccagaacg
      721 aagaggcgta cccgatcggc gacggtgcgg tcgacctgat cgtatccaac gcgctgccgc
      781 aggagccgcc actgaagaag aatcagaagg gctatctgga cgcggacagt tcactgtatc
      841 cgcaccagct ctacctgtat ggatgggtga ccgagcagat ctggaacgag atcaagccgc
      901 acttgtattc gccgaccccg aactgccaga ccgacctgat cctgcgcgac aactgcatgt
      961 ttccggtcaa gcagggtttt gcgttcgtcg ccggtccgcc tggagcggtc ggcctcacga
     1021 atgtcgagtt ccgcgtcgcg tcgcattccg ccgagcgtcg caccagccgt cgcgccgaga
     1081 ccctgcaacg ctgacggtca ccgcacggaa tgcggcgttg cagcaaatca tgattgagat
     1141 tgcgcgggcc tcactctaaa atagtgtttg ttgcttcgca acatagaggc cggccgcgcc
     1201 ggcaccctga tcagaatcag cacggagaag ccatgttccc ctctcaacag cagatcgctg
     1261 ccgcggcgcg cgcgcagttc gacacgcagg tggcgctgta ccgcgagttc agccagacgg
     1321 cgctggacag catggaaaag ctcacgcggc tcaatatcgc tgccgtccgc gcctcgatgg
     1381 aagagaattc ggccttggcc cggcagatgc tgaccgcgcg cgacatgcag caggcagtgt
     1441 cggtcatgcg cacgcagacc ggtccgagta tcggcaaggc gatcgcctac ggcaatcatg
     1501 tggtcaacat tgcgtccgat gcgcaggcgg atctgacgcg cgcgaccgag gttcagcttg
     1561 ctgaggccgc gcggcgtgcc ggcgagctgg tggatgaagc cgcccgcaat gcgccgccgg
     1621 gaacggaaaa cctgatggcc ctcgtgaagg tcgcgatcgg cagcgcaacc gccggatacg
     1681 aagaggtcaa ccgttcgacc cggcgcgcgg tccagacgct cgaatcgaac gtcaacgccg
     1741 cggtcaatca aatcgtccag cccgctccga ccacgggagg ctgagccgga acgccgcgcg
     1801 cggcggcatt gagaagttaa gacaagaagc cgatgtcaag gcgccggcgc agacagtgcg
     1861 tgtagtacgg ctgggaggtg caacgccgca agcggcttct tgttgtggct caggcgccca
     1921 gcgactgtgc cgccttgtcg agccaggttg cgaggctcgc gcgcaattcc tcctggctga
     1981 atgagcagct ctcttcaccg aacaggctgc cgctttccat ccgtccgagc agatcctcca
     2041 tcaccaggcc ataacgtgcg ggcaggcacg ccagcagggc gtgctgggcg cgccagatgc
     2101 ggcaaaacgc gtcggtcgcg atgcgtcttt gcgcgagcgc gtcgagcgac gcgcgcagcg
     2161 aggcgagcgc ttcggcgtgg tcaggggtag ctgcggtatc agtggcatgc atgcggtgtc
     2221 tcagaatggc tgcttggtca gcgcgagctt gaggatgtag gcgaagattg caagtgcggt
     2281 cacgaacgcg accccgcgca cgaagcggga gctgccgcgc ttgagcgcga tgctgccgac
     2341 gccgatgtag gcgaacacgg cgatcacctt cacggtcagc cagtcctgca ggagcgggta
     2401 acgtgcactc cacaccacca ggccgattgc gctgacgagc aggacggtgt cgattacatg
     2461 cgcggccagg cgcagcgaac ggcgttgcgc aagcggggac gcctgcagca tccacagacc
     2521 gcgcatgccg aacagcaggc cgctcaccag ggcgcaggcg acgtgcagat gcttgagcgt
     2581 gtaataggtc atcgcgatgc tacaggccgg cttccgcttg cggaatggcg tcctcgatgc
     2641 ggtaccagct cgccttgaac gcggcgtaga tgtgctgctg cggcggtggg ggcggcgagg
     2701 tgtccagggt gccggcggca atgctgaccg tttccggatg gtggatcgtg ctgtaccact
     2761 gcagcgtcga accgcagcgc ttgcagaagg tgcgggtcac gcccggggaa gaggagtacg
     2821 cggcgaccat gtcctgccct tcggtgaagc ggaaggcgcc gtgcgcgact gtgacgtaag
     2881 agccgaaggc cgcgccgtgc gccttgcggc acatcgagca gtggcaatgg gtgatgcgct
     2941 tgatcggcgc cgtgatctca tagcggatcg ccccgcacag acaactgcct tgatgcatga
     3001 tgccctccct ggtcgcgaac ggcatcattc tagcaagaca cccgcgtccc ggcggcaggg
     3061 caattaacgc gtcagccgtt gtccttgtct gcggcgccgc gcgcctgggc atccccccct
     3121 tgctcttcgc gccgaagctc ctgctggcgc cgccacggcc gcccgatgcg ctgctccacc
     3181 gacgcgtgct cctcgaacgg gcgcgcggtc gcaagcttga gggcgatgcc ggtgcccagc
     3241 cgttcacggt aggtgtcggg gaggtccaca ccatagctcc agccgctgcc attcatccag
     3301 aaccagatct gctgctgggt ggagtagtag acctgcagat ccgggtaata gacatagcgg
     3361 accaggacgg gagccgcgag ccgcgccggc acgctggaag cgcctgcctg ggcgtaagct
     3421 ggcgcatgcg gtgcgactcc gagggccgcc aaggtgagca ggccggccgg cagcgaacgg
     3481 gtgcgacgga acgaaggggc ggacggcatg gcatctccag tcgggaaacg gaatgccgat
     3541 gatcgcccgc aggctcggca cagcggaaga attgttgttt ttgattgtta cggtggcaac
     3601 cgggtgaagc cgattttcgg ttgccaacgt aacatccggt tgcaaaatca gaaaggctca
     3661 gctcttcggc cacagaacca tctgcgtgca ccggaaggtc gcgatcagct tgtccgtctc
     3721 gcgatgacgc accagcgcgt cccagacctg ggtgttgcgc ccggtgtgga ccgcctgcgc
     3781 ggtgcagtcg atcgtgccct cgcgtgcggt gccgacgaaa ttgctcttga gctcgatcgt
     3841 cgtgaagctg accgcgcctt cgggcaggtg cgccatgcag gcatagccgc aggcggtgtc
     3901 ggccagggtg acgatggtgc cggcatgcag atagccgttc ggcgccagca gctgcggccg
     3961 gaccgccaga tgggcttgca gcgtcttgtc gccgatttcg gtgacgacga tgccgaactg
     4021 cccgggcagg cagtcggcgc cgacgcggtt gaagtggtcc actgtcattc gggtctgcat
     4081 ggacgatcct ttgctgatgt gtgagggtgg atgaattctt gcatattcgc ccggcccgtt
     4141 caggagcctg ctccagaaga acattgaact tcgaacccgt gccgtccttc caattggctt
     4201 tcgaaaaacc gggagtgcga gatgaatgtt ctttccaagc atgagactga agcgcttgcc
     4261 cggcatgtcg ccgaaggcgc cggcgccccc ggtgccggca tgcgcaccca gacggcatta
     4321 attgcagccg gcgctgcgct ggcggcaacc gcgctggtgg tgcagcgtcg cagccaggac
     4381 gtcgagcgga cccatccgcc aatcggcaag ttcatcgagg tcgatggcgt gcgcctgcac
     4441 tacaccgagc gaggcgacgg gcagccgctg gtgatgctgc atggcgtcgg tgcaatgatg
     4501 caggacctga tcatttcagg aatcgtcgac atggccgcca gcaagtacag ggtcatcctc
     4561 ttcgaccggc ccggctacgg ttacagcgaa cggccgcgca cgacgatctg gacgccccac
     4621 gcgcaggcac gcctgctgca caaggccttg cagcagatgg gcattgcgca gccgatcgtg
     4681 ttcgcgcatt cctgggccgg catggtcgcc acggcgatgg cgctggattt tccggacgac
     4741 gtgaagagcc tggtgctgct gtcgggctat tactacccga cagcccgtct cgatatcccc
     4801 tacatggccg cgccgtcgat cccgctggtg ggtgacctga tgcgctacac gatctcgccg
     4861 ctgctcgggc gggcgatctg gccggcaatg aagcgcaagc tgtttgcgcc ggcaccggtg
     4921 ccgcgccgct tcgagcagga attcccgacc tggatgacgc tgcggccgtc gcaactgcgc
     4981 gcctccgcag ccgagtacgc gctgctgatt ccggcggcct acgcgatgca ggcgcgctat
     5041 cacgagctgc tgctgccggt tgtcatcatg gccggcgacg gcgaccgcca cgtcgacacg
     5101 cgcctgcatt ccgaaagact gcatcacgag ctgccgcaca gcagcttcct ggtcgcgcac
     5161 ggggcgggcc acatggtgca ccactgtgcg caggatcagg tgatggccgc gatcgacgag
     5221 gcggcgacgg cggtgggggc ggttccggtc gaacaccaga gggcccaggc cgggcagtcc
     5281 tacgtgtttc cttcgcccgg ccaggtcagc tgaacagcca gcggtagcgg ccgggtgcgg
     5341 tgccgactgc ggcccggaaa cgatggctga aatggctcag gtccgcatac ccgcaggcgt
     5401 cggcgacctg ctgcagcggc aggttggcgg cgcccagcaa ttggcgggcc ctgtcgatac
     5461 ggcgcgcggc gatccatgca tggggcggca tgccgaatga aacgcggaac atgcgtgcga
     5521 ggtggtactc cgacaggcag gcgagctctg ccagcatgcc gagcgtgatc ggcaagtgca
     5581 gatgggcttc gatgtaatcc gacagtcgcc tgcgcacggc cggggccagt ccgcccctga
     5641 ggcgctcgcc gccgcggcgc ggcgcggatt gcgacagcag cagctgcgac agcgccgcat
     5701 gcgcggtctc gttcgcgcgc aactgcgcgt cggccccgtc ccatggcatc gcggccagcc
     5761 gctcgcacaa ccgctcgatc gtcggattgg cgaaataggt gcggtcggcc agcgtcactt
     5821 cgcgcggctc gcgatccagc tccagcaccg cgcggcgggt gaagtgccgg ggcatgaagt
     5881 acaggtgcag aaagcgcagg tcgccgcgga tcagccagcg cgaatcatgg tcgtccggca
     5941 gcgtgcacag catgcgcggc gcgccatatt ggccgggaag ctcgctgcgc tgcgtgcggt
     6001 agcctccgcc cagatagaac gacagcgtgt ggtgtccggg ctgccggtac gcggtctcgg
     6061 ccgcatcggt ctgacgcttc cagatcgcga ccgcgagttc gtcgcccagc caggcgaagc
     6121 gctccagttc cgcgttcgtc ccgtgcaggg tgcgaaagac ggcgtgcggc atcgcgccgt
     6181 tcggcgaggc gggagcaggc ttgtgcagga ctggatcgga catgcgatgt cggcgtgatc
     6241 ggcggctcag ccaatgtagc atgcgcatcc tttaacgtgc cggcccggcg cgcccggccg
     6301 ccaataatgc gcaagatcgg acaatccggc ccggacggtg aaaggatgcg caatttcgga
     6361 caagcgcgcc gtcgcgcgac gcgtcatgct ggcgtccttt cttcaccgat gccagcgcat
     6421 gaacgcgtct ctctacatcc tcactgtcct gatctggggc tcgacctgga tcgccatcaa
     6481 gtggcagctc ggcgtggtgc cggcgccagt ctcgatcgcc tggcgattct ggatcgcggg
     6541 cgccgtcctg ctcgcgctcc tggccatcct gcgcaagccg atgcgtccgc cgcgcgcggc
     6601 ctttccgtat cttgccgccc agggcgtcgc gctgttctgc tgcaatttcc tgtgcttcta
     6661 ctacgccagc cagtgggtgg cgagcggcct ggtcgcggtc gtgttttcca ccgcgacgct
     6721 gtggaacgcg atcaatggcc gcattttcct cggacgggca ttgcagccgc aggtgctcgc
     6781 cggcgccggg atgggcatcg tcggcatcgt gctgctgttc ttgccgcaga tgtcgggaca
     6841 ctggacggac gccggcgtgc tgcccggcct cggcctcgcg ctgctgggca cgctgtgctt
     6901 ctcgaccggg aacatgctct ccgcgcgcat gcaggcgatg gggctcacgc cctggatgac
     6961 caatacctgg gcgatgttca tcggcgccgg cgtgctgact gtgatagcgc tggttctcgg
     7021 catgccgttc gcgctcgaca ctagcgcgcg ctacctcggg tcgctgctgt acctcgcgat
     7081 tcccggatcg gtgatcggct ttaccgccta cctgatgctg gtcggacgca tgggcccgga
     7141 gcgcgccgcc tattgcaccg tgctgttccc ggtcgtagcc ctggccttgt ccacgctgtt
     7201 cgaaggctat gtgtgggacg tctgggccgt tgccggactc gtgctcgttc tttgcgggaa
     7261 tctgctcgcg ttcatgccgc tgcgctggct gcggccgagc ccggcctagt aggtgctgcg
     7321 atagcgatgg ccgcgatagt tcagcaccat gccgtccggc tgcagcgatt ccagcaccag
     7381 tccgggcgcg atctcgtcgc cctcgcggcg caggcgctgg ttgatcagca cggtacggtc
     7441 cgcgggattg gccgcgtaca gatagccgcc gatcaccaat ggcggaatct cgcgctggat
     7501 tgcttccggc aggtcacgca tcgtgccggg ccgcgcggag accgtttgcg cgggcggggc
     7561 aggtgaacgc gcaggcgcgg cggcgcgcac cggcggcgcg ggcgcgcgga cgatcgggct
     7621 cggcccggat ggcggcggaa tgatggacgc ctccgcggat gccgccggtg cctgcggttc
     7681 ggctacggcg ggcagcacct cggcgggcgg tgcctctgtg gtcggatcgg cgcgcaggac
     7741 gaaggctgct gcgccgatca ggcccgccag cagcgcggca cccagcggca atgcgaaccg
     7801 gtgcgcgagt cgccgcgtcg caggcggagc ggcgtatgcc gacggcagcg gcgccggcgc
     7861 cgccacgcct gcgatgcgtt cggcttcggc ccgtttcaat gcgtcgagaa tgtaggacat
     7921 caggaacccg cttcgttcag acgcggttcg gcgacgccgg ccgcgcggtt caggtgcatc
     7981 agcgtcaccg gcccgaccac gccgtcgacc gccagcccct gcgattgctg gaacaggcgc
     8041 acctgccggt gcagctcgag attgaacggc tggcgcgcgg gcggcagctc ggtgccgtgc
     8101 accctggcga gctgggtgcc gacccagtcg accgccgcgc cttgctggcc gagcgcaatc
     8161 gttgcaccca ggcccggggg cgtttgccac agcgtgacga attctcctcg gaagtgacgc
     8221 gtcaggtcgg ccaggccgac ttcctgatgc gcgcctgccg cctgcagccg gtagcgcccg
     8281 ccctgggcat gcacgagcag cgcgtgatag
//
LOCUS       DATLGF010000393         7739 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_172079, whole genome shotgun sequence.
ACCESSION   DATLGF010000393 DATLGF010000000
VERSION     DATLGF010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 7739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7739
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172079"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VM406_12010"
     CDS             complement(<1..475)
                     /locus_tag="VM406_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HVL76731.1"
                     /translation="MSDINSEFHRNKALALLKDAELVFDAQAVQQAVDKVAEQLNHRF
                     KDNADAPFPLVLGVMGGAVVFCGQLLTRLDFPLEFDYMHVTRYGDKDQGGKIEWKVIP
                     RSSVEGRTVIVLDDILDEGETLAHVKERLLEMGAAEVVLAVFADKAIGKSKPVTAD"
     gene            638..940
                     /locus_tag="VM406_12015"
     CDS             638..940
                     /locus_tag="VM406_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HVL76732.1"
                     /translation="MKKAYVVAEIQVTNPQEYEGYRTLSTAAAAQYGGQFVVRGGARE
                     QLEGEDPQHNGGWRTVIIEFPSLEQARTWYHSPEYTKAREIRQANSVGRLFLIEGA"
     gene            1031..1333
                     /locus_tag="VM406_12020"
     CDS             1031..1333
                     /locus_tag="VM406_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76733.1"
                     /translation="MTSSAKPGMPATPDSDRTMKQHQVRTGRPIHEDEAMRMPHERDE
                     SEDSQESEPRKVIKRAYDDLMEGQVDTDLRETGGLDAAVNERPGIPPEKVVGKGAT"
     gene            1473..2594
                     /gene="moaA"
                     /locus_tag="VM406_12025"
     CDS             1473..2594
                     /gene="moaA"
                     /locus_tag="VM406_12025"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HVL76734.1"
                     /translation="MQEQVVHWIHAGRTAAELSGVAPEPLALPDLPPLLQDKLARPLH
                     DLRISVTDRCNFRCTYCMPKEVFDKDFPYLPSAAMLDFDEIERVARAAVGLGVRKLRL
                     TGGEPLLRKDVETLIARLAALRAPDGEAIELAMTTNASLLKRKAAGLRAAGLNRVTVS
                     LDAIEDATFRRMNDVDFPVRDVLDGIEAAVAAGLGPVKVNVVVKRGVNDNQILPIVAH
                     FRHRGVVLRFIEFMDVGATNGWQMQHVLPAAELRARIADRYELVPLEPSSSGETAQRY
                     MLADGSLEIGTISSISEPFCGDCNRARLSTDGKLFLCLFAVKGFDIRHMLRGGVSDDE
                     LARTIAGIWTQRRDRYSELRAEVGDRRRQAKVEMSYIGG"
     gene            complement(2768..4015)
                     /locus_tag="VM406_12030"
     CDS             complement(2768..4015)
                     /locus_tag="VM406_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVL76735.1"
                     /translation="MNDMLDELESRDPAQRERELMSRLPQLVARAQKAPGWARILAGV
                     NAADITSRAALAALPVTRKSDLKDLQAGDAPFGGLTTTPTRELRRLFMSPGPIFDPEG
                     RGEDWWRFARPMRAVGLRAGDIVQNCFSYHFTPAAFMAEGGAAKIGCAVIPAGIGQTE
                     MQVQAMVALRPDAYVGTPSFLKIIIEKAREMDADISSVQRAVVGAEALPPSLRKWLQD
                     NGVPHVLQMYGSADVGNIAYETETDGQVNPGMVLDEDLILEIVRPGTGDPVAEGEVGE
                     VVVTSFNPDYPLIRFGTGDMSAILPGVSPCGRTNTRIKGWMGRADQTTKIRAMFVHPG
                     QVAEVVKRHPQIARARLVVSGEMANDIMTLHCEVDDPAAAQADGIVDTIRDITKLRGE
                     VKFVARGSLPNDGKVIEDARKYE"
     gene            complement(4188..5024)
                     /locus_tag="VM406_12035"
     CDS             complement(4188..5024)
                     /locus_tag="VM406_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL76736.1"
                     /translation="MNLAPATPSPSAPAYLSVNNIEVIYDHVILVLKGVSLQVPQGKI
                     VALLGANGAGKSTTLKSISTLLRGERGDVTKGSIEFMGDRIDQLTPNELVRRGLAQVM
                     EGRHCFGHLTIEENLLTGAYTRKISRAQLKESLEMVYHYFPRLKQRRTSQAGYTSGGE
                     QQMCAIGRALMAKPSMILLDEPSMGLAPQIVEEIFEIVKDLNSKENVSFLLAEQNTNV
                     ALRYADFGYILENGRVVMEGDAKDLAANEDVKEFYLGVSGAGRKSFRDMKFYRRRKRW
                     LA"
     gene            complement(5094..6398)
                     /locus_tag="VM406_12040"
     CDS             complement(5094..6398)
                     /locus_tag="VM406_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL76737.1"
                     /translation="MKLLKNMLLGAVMAGTVSAAVAQQQFIPILSYRVGPYASGGSGF
                     FGGAIDYMNLVNANGGINGVKLAWEECETEYNASRGVECYERLKSRHGGASLVEPLAT
                     GIAYGILDRVAVDKIPMTTLGYGRSDAANGKVFPYVFPLITSYWNQAAAMIAYLGSKE
                     GGVANLKGKKIVHLYHDSAFGKEPFPVFDALAQRHGFELIKIPVAHPGNEQQSQWLQI
                     RQARPDYVILWGWGVMNAVALTTAQRNGFPREKILGVWWAGSEEDTIAAGPAAKGYTA
                     MTFNTPGNYPVLDQIRKQVYDAGRGNLNDRNRIGSVYHMRGVTAAILWTEAIRTAQEK
                     FGKGKPLTGEQIRWGFENLNVNEARQKELGALGMFPPVRTSCDDHEGSGAVKVQQWDG
                     DKWVAITPMWVTGDKALTRKLLEESSAKYAQEKNITPACLNQ"
     gene            complement(6482..7540)
                     /locus_tag="VM406_12045"
     CDS             complement(6482..7540)
                     /locus_tag="VM406_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVL76738.1"
                     /translation="MFYREAGQFKPTYAADSQIFPIRQDRIGIILLLAIAFIVIPGMA
                     SNYMFSAILIPFLIFSLAALGLNILTGYAGQLSLGSAAFMAVGAFASYNFMLRVDGMP
                     ILVAFILGGLCAAAVGVLFGLPSLRIRGFYLAASTLATQFFVVWALTKIGWFTNYSSS
                     GVITAQRINILGYDFNTPASKYLLVLTIVCVMALLAKNMVRSNVGRSWMAVRDMDVAA
                     EVIGFRLMRTKLLAFAVSSFYCGVAGALYAYAYLGTVEPEAYSLDLSFRILFMIIIGG
                     VGTIIGSFFGAGFIVLLPIGLSLLAHNFGLPNSVTSNLELMVFGALIIFFLIVEPHGL
                     ARLWQIAKEKLRLWPFPH"
BASE COUNT         1339 a         2501 c         2534 g         1365 t
ORIGIN      
        1 aatcggccgt gaccggcttg ctcttgccga tagccttgtc ggcgaacacg gccagcacga
       61 cctcggcggc gcccatctcc agcaggcgct ccttcacatg ggcgagcgtc tcgccttcgt
      121 cgaggatgtc atcgagcacg atcaccgtgc ggccctccac gctggagcgc ggaatcacct
      181 tccattcgat cttgccgccc tggtccttgt cgccgtagcg ggtgacatgc atgtagtcga
      241 attcgagcgg gaaatccagg cgggtcagca gctggccgca gaacaccacg gctccgccca
      301 tcacgccgag caccagcggg aagggcgcgt cggcgttgtc cttgaatcga tggttcagtt
      361 gctcggccac cttgtccacg gcctgctgca ctgcctgcgc atcgaacacc agttcggcgt
      421 ccttgagcag cgccagagcc ttgttgcggt ggaattcgga attgatatcg gacatggagg
      481 gccttcagac aatggaaacg caacgtacct ggaacaccga gcaggattga tgccgggcgc
      541 catgatatgc cgggcccgcc cggcgtgcaa acctgagcca aactacaatc ggcactgcgg
      601 ccgcgcggcg gctctttgaa aaaacggagg gaaggacatg aaaaaagcgt acgtggtagc
      661 agaaatacaa gtcaccaacc cgcaggaata cgagggctac cgcaccctct cgaccgccgc
      721 cgccgcccag tacggcggcc agttcgtcgt gcgcggcggc gcgcgcgagc agctggaggg
      781 cgaggacccg cagcacaacg gcggctggcg caccgtcatc atcgagtttc cgtcgctcga
      841 gcaggcccgc acctggtacc actctccgga atacacgaag gcccgcgaga tccgccaggc
      901 caattcggtt ggccgcctgt tcctgatcga gggcgcctga gcatttgagc aacgccggaa
      961 atcgagcgcg gcgggggaat tatcgcttgg gcatggcctc caataatttc aatgtctgtt
     1021 ggagagcacc atgaccagct cggcaaaacc cggcatgccg gcaacgccgg attccgaccg
     1081 cacgatgaag cagcaccagg tgcgcaccgg tcgaccgatc cacgaggacg aggcgatgcg
     1141 catgccgcat gagcgcgacg aatccgagga ttcacaggaa agcgagccgc gcaaggtgat
     1201 caagcgcgcc tacgacgact tgatggaggg gcaggtcgac accgacctgc gcgaaaccgg
     1261 tggcctggat gcggcggtca atgagcggcc cggcattcct ccggaaaaag tggtgggcaa
     1321 gggcgcgacc tgacgcgccg ctgttgtcgc gaggaacgat ttcctgctgt tgcgtgaagg
     1381 gcgggccggc cgcgccggcc cgtccgcgcg tccgcaccgg tcctgaaagg acgcgtgcca
     1441 tgcgctaaca tggccgctca ccggagaaat tcatgcagga gcaggtcgtt cattggatcc
     1501 acgcggggcg taccgcggcc gagctgtcgg gcgtcgcccc ggagccgctc gccctgcccg
     1561 acctgccgcc cctgctgcag gacaagctcg ccagaccgct gcatgacctg cgcatctcgg
     1621 tcaccgaccg ctgcaatttc cgctgcacgt attgcatgcc gaaggaagtc ttcgacaagg
     1681 acttccccta cctgccttcg gcggcgatgc tcgacttcga cgagatcgag cgcgtcgcgc
     1741 gtgcggcggt cgggcttggc gtgcgcaagc tgcgcctgac cggcggcgag ccgctgctgc
     1801 gcaaggatgt ggaaaccctg atcgcccgcc tggccgcgct gcgcgcaccc gacggcgagg
     1861 cgatcgaact ggcgatgacg accaatgcca gtctgctcaa gcgcaaggcg gccggcctgc
     1921 gcgccgccgg gctgaaccgg gtgacggtga gcctggacgc gatcgaggat gcgaccttcc
     1981 ggcgcatgaa cgacgtcgat ttcccggtgc gcgacgtgct cgacgggatc gaggcggcag
     2041 tggctgcggg gctgggcccg gtgaaggtca acgtggtggt caagcgcggc gtcaacgaca
     2101 accagatcct gcccatcgtc gcgcatttcc gccaccgcgg cgtggtgctg cgcttcatcg
     2161 agttcatgga cgtgggagcg accaacggct ggcagatgca gcatgtgctg cccgccgccg
     2221 agctgcgcgc ccgcatcgcc gatcgctacg aactggttcc gctcgagccc agcagcagcg
     2281 gcgaaaccgc ccagcgctac atgctcgccg acggcagtct cgagatcggc accatctcca
     2341 gcatcagcga gccgttctgc ggcgactgca accgcgcccg cctgtccacc gacggcaagc
     2401 tcttcctatg cctgttcgcg gtcaagggct tcgacatccg ccacatgctg cgcggcggcg
     2461 tcagcgacga cgaactcgcg cgcacgatcg ccggcatctg gacgcaacgg cgcgaccgct
     2521 attccgaact gcgcgccgag gtcggagacc ggcgtcggca ggcgaaggtg gagatgtcgt
     2581 acatcggggg gtgaggcgga ggttcgtttg ggacacggcg aaaaccccaa taactgtcat
     2641 cccgagcgct gcgagggatc tcatcggatg acagaaccgg gattcctcgc tgcgctcgga
     2701 atgacaggtt ttcgttgcgg ttcaaagacc ttacaccgca tccgccacgg ttcaggaacg
     2761 gccaccctca ctcatacttg cgcgcatcct cgatcacctt gccatcgttc ggcaggcttc
     2821 cgcgggcgac gaacttcact tcgccgcgca gcttcgtgat gtcacggatg gtatcgacga
     2881 tgccgtccgc ctgcgcggcc gcgggatcgt cgacttcgca gtgcagcgtc atgatgtcgt
     2941 tggccatctc gccgctgacc accagacgcg cccgggcgat ctgcggatgg cgcttgacca
     3001 cttcggcgac ctggccggga tgcacgaaca tcgcgcggat cttcgtggtc tggtcggcgc
     3061 gccccatcca gcccttgatg cgcgtattgg tgcgcccgca cggcgacacg cccggcagga
     3121 tcgcggacat gtcgccggtg ccgaagcgga tcaggggata gtccgggttg aacgaggtga
     3181 ctaccacctc gccgacttcg ccctcggcca ccgggtcgcc ggttcccggg cgcacgatct
     3241 ccaggatcag gtcttcgtcg agcaccatgc cgggattgac ctggccgtcg gtttcggtct
     3301 cgtaggcgat gttgcccacg tcggccgaac cgtacatctg cagcacgtgc ggcacgccgt
     3361 tgtcctgcag ccatttgcgc agcgagggcg gcagcgcttc cgcgccgacc accgcgcgct
     3421 gcacgctgct gatgtccgcg tccatctcgc gcgccttctc gatgatgatc ttcaggaacg
     3481 acggcgtgcc gacgtaggcg tcgggtctca gggccaccat cgcctgcacc tgcatctcgg
     3541 tctggccgat gccggccggg atcaccgcgc agccgatctt tgccgcgccg ccctcggcca
     3601 tgaaggccgc cggggtgaag tgatacgaga agcagttctg cacgatgtcg ccggcgcgca
     3661 ggcctactgc gcgcatcggt cgcgcaaagc gccaccagtc ctcgccgcgg ccttccggat
     3721 cgaagatcgg tccgggcgac atgaacagcc ggcgcagctc acgcgtgggt gtggtggtca
     3781 ggccgccgaa cggcgcatcg cccgcctgca ggtctttcag atcggatttg cgcgtgaccg
     3841 gcagcgccgc cagcgccgcg cgcgaggtga tgtcggccgc gttcacgccg gccaggatcc
     3901 gtgcccagcc cggcgccttc tgcgcgcgcg cgacgagttg cggcaatcgt gacatcagtt
     3961 cgcgctcgcg ttgtgcggga tcgcgcgatt cgagttcatc cagcatgtcg ttcatggtcg
     4021 gagccgtccc cagttgcgcg catctcgcgc tgcgcgcgcg tcatgttcaa gattggggcc
     4081 gcgcgagcgg ctctcacttc gttctttcca ggagccgggc aaaaagaggc ccctgcgcca
     4141 gcgcgcatgc ggtggcgtgt caagcgacgc gacggatgcg gccgcgccta tgcgagccag
     4201 cgcttgcgcc ggcggtaaaa cttcatgtcg cggaagctct tgcgtccggc gccggagacg
     4261 ccgagataga actccttcac atcttcattg gcggccaggt ccttggcgtc gccttccatc
     4321 accacgcggc cgttctccag gatgtagccg aagtccgcgt agcgcagcgc gacgttcgtg
     4381 ttctgttcag cgagcaggaa ggagacgttt tccttgctgt tcaggtcctt cacgatctcg
     4441 aagatttcct cgacgatctg aggagccagc cccatcgacg gctcgtccag caggatcatc
     4501 gacggcttgg ccatcagcgc gcgtccgatc gcgcacatct gctgttcgcc gcccgaggtg
     4561 tagccggcct gggatgttct gcgctgcttc aggcgaggga agtagtggta gaccatctcc
     4621 agcgactcct tcagctgggc gcgggagatc ttgcgcgtgt acgcgccggt cagcaggttt
     4681 tcctcgatgg tgaggtggcc gaagcaatgg cggccttcca tcacctgggc caggccgcgc
     4741 ctgaccagct cgttcggcgt cagctggtcg atgcggtcgc ccatgaattc gatgctgccc
     4801 ttcgtcacat cgccgcgctc gccgcgcagc agtgtcgaaa tggatttcag cgtggtggat
     4861 ttgcccgcac cgttcgcacc cagcagcgcg acgatcttgc cttgcgggac ttgcagcgag
     4921 acgcccttca ataccaggat cacgtggtcg tagatgacct cgatgttgtt gaccgacagg
     4981 taagcgggag cgctcgggct tggagtggcg ggtgcgaggt tcatggtcat cttcattcgg
     5041 ctgctgcccc ggccctcccc ctttgcgggg gagggccggg gtcgtttacg cgattactgg
     5101 ttcaggcacg ccggagtgat gttcttctcc tgcgcgtact tggcggagga ttcctccagc
     5161 agcttgcgcg tgagcgcctt gtcgccggtg acccacatcg gggtgatcgc gacccacttg
     5221 tcgccatccc actgctggac cttaactgcg cccgagcctt cgtggtcgtc acaggaggtg
     5281 cgcaccggcg ggaacatgcc gagtgcgccc agttccttct ggcgcgcttc gttgacgttc
     5341 aggttctcga agccccagcg gatctgctcg ccggtcagcg gcttgccctt gccgaacttc
     5401 tcctgcgcgg tgcggatcgc ctcggtccac aggatcgccg cggtcacgcc gcgcatgtgg
     5461 tagaccgagc cgatgcggtt gcggtcgttg aggttgccgc ggccggcgtc atacacctgc
     5521 ttgcggatct ggtcgagcac cggatagttg cccggggtgt tgaaggtcat cgcggtgtag
     5581 cccttggctg ccggaccggc tgcgatcgtg tcttcttccg aaccggccca ccacacgccg
     5641 aggatcttct cgcgcgggaa gccgttgcgc tgggcggtgg tcagcgccac cgcgttcatc
     5701 acgccccaac cccacaggat cacgtagtcc ggacgcgcct ggcgaatctg cagccactgc
     5761 gactgctgtt cgttgcccgg gtgcgcaacc gggattttga tcagctcgaa gccatggcgc
     5821 tgggccagcg catcgaacac cgggaacggt tccttgccga acgcggaatc gtggtacagg
     5881 tgcacgatct tcttgccctt caggttggcc acgccgcctt ccttgctgcc gaggtaggcg
     5941 atcatcgccg ccgcctggtt ccagtagctg gtgatcagcg ggaagacgta cgggaagacc
     6001 ttgccgttgg cggcgtcgga gcggccgtag cccagtgtgg tcatcgggat cttgtcgacc
     6061 gcgacgcgat ccaggatgcc gtaggcgatg ccggtagcca gcggttcgac cagcgaggcg
     6121 ccgccatggc gcgacttcag gcgctcgtag cattccacgc cgcgcgaggc gttgtactcg
     6181 gtttcgcact cttcccacgc cagcttcacg ccgttgatgc cgccgttggc gttcaccagg
     6241 ttcatgtagt cgatcgcgcc gccgaagaag ccggagccgc cggaggcata cggaccgacg
     6301 cggtaggaca ggatgggaat gaattgctgt tgcgcgacgg cggcactgac cgtgcccgcc
     6361 atcactgcgc ccagcagcat gtttttcaag agtttcatgg tggtctcctc catataggga
     6421 tacagctggt acaactcagg gtggcctgga agcctgcgct gccaggccgg ggtcgatgcg
     6481 gtcagtgcgg gaagggccac aaccgcagtt tttcctttgc gatttgccac aggcgggcga
     6541 ggccgtgcgg ctcaacgatc aggaagaaga tgatcagcgc gccgaacacc atcagctcca
     6601 ggttcgacgt caccgagttc ggcaatccga agttgtgcgc gagcagcgac aggccgatcg
     6661 gcagcagcac gatgaagccg gcgccgaaga acgagccgat gatggtgccg acgccgccga
     6721 tgatgatcat gaacaggatg cggaacgaca ggtccaggct gtaggcttcc ggctccaccg
     6781 tgccgaggta ggcatacgca tacagcgcgc cggccacgcc gcagtagaac gaactgacgg
     6841 caaacgcgag tagcttggtg cgcatcaacc ggaagccgat cacctcggcc gcgacgtcca
     6901 tgtcgcgcac cgccatccac gagcgcccga cattggagcg caccatgttc ttcgccaaga
     6961 gcgccatcac gcagacgatg gtcagcacca gcaggtattt cgaagcaggg gtgttgaagt
     7021 cgtagccgag gatgttgatg cgctgcgcgg tgatcacgcc cgagctgctg tagttggtga
     7081 accagccgat cttggtcagc gcccagacga cgaagaactg cgtggccagc gtcgaggccg
     7141 ccaggtagaa gccgcggatg cgcagcgagg gcaggccgaa cagcacgccg accgcagctg
     7201 cgcacaagcc gccgaggatg aaggccacca ggatcggcat gccgtccacg cgcagcatga
     7261 agttgtagga ggcgaacgcg cccaccgcca tgaaggccgc cgagccgagc gagagctggc
     7321 cggcgtagcc ggtcaggatg ttcaggccga gcgcggcgag cgaaaagatc aggaacggga
     7381 tcaggatcgc cgagaacatg tagttcgacg ccatgccggg gatcacgatg aaggcgatcg
     7441 ccagcaacag gatgatcccg atgcggtcct gccggatcgg gaagatctgg ctgtcggctg
     7501 cgtaggtcgg tttgaattgg cctgcttcgc ggtagaacat ggtggtttcc ttgtttcgct
     7561 atctaagtcc tccggctttg ttccccccct tccagggggg tgaagccagg gtttcggacg
     7621 acatgtcgtc cgctggcgga acggtgcggg cttgcaagcc cgtacaccgt ttccatccag
     7681 ggagggggta cggagcttgt tatgaagcca tcgcatgctc cagccccgca cccccatcc
//
LOCUS       DATLGF010000394        12296 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_8778,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000394 DATLGF010000000
VERSION     DATLGF010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 12296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12296
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8778"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VM406_12050"
     CDS             <1..307
                     /locus_tag="VM406_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL76739.1"
                     /translation="RAAGVQPQVRLFFTDMGEDLVIEVDDNGRGVPDELAGAIFDAGV
                     STKGAGRGIGLALVRRLCQQHGGDATLEASELGGACFIAVVDKHAVGAPSDLEVRHG"
     gene            300..1013
                     /locus_tag="VM406_12055"
     CDS             300..1013
                     /locus_tag="VM406_12055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL76740.1"
                     /translation="MAEPYRVLIVEDDFRIADINRSFVEQAEDFTVVGMARSRAEALA
                     WFDAHPGGVDLLLLDVFLPDVEGLALLWEIRRRHRDLDIVMVTAAREVETVEEALRGG
                     VFDFLIKPVEATRVQKMLARFRSRRQLADGRRELSQEQLDHALSSLYASPGASARRAL
                     PKGIDALTLDTVLEVLRQCEGALTATELAGRIGSSRSTARRYLEHLVGEQLVSAEQSY
                     GDVGRPQRQYRLRTRRGAA"
     gene            1214..2218
                     /locus_tag="VM406_12060"
     CDS             1214..2218
                     /locus_tag="VM406_12060"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVL76741.1"
                     /translation="MFNPRVLPCMRALMRGTAAAMLLGAAAVPALAQDWQPTRPIEYI
                     APANPGGGWDTLVRTTARVIQEEKLAPQNFAPINIPGGGGAVAWAQIAAQKGNPHRLF
                     AASPPIILVPLSGASRFTHKDFTPIARLITDYSILLVRADSPYKTANDLFNAIKERPG
                     LSVGGGSAPGSMDHISMAGAASAAGLKPAQVNYIAFSGGGEAMTNLLGGHVAAVITGA
                     GEAQAQLKGGQIRALAVSSPKRIASLPNVPTYQEQGINYTFDIWRGIMGPPNMPPEAV
                     RYYENMYARMLKTDGWAKARDQLGWIDAYQNSAEFGKFLDAQHAQFSSVLQDLGLLRR
                     "
     gene            2299..2766
                     /locus_tag="VM406_12065"
     CDS             2299..2766
                     /locus_tag="VM406_12065"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HVL76742.1"
                     /translation="MKRLNINQVLAILIAALSFAYLFEAYRIPTFPIPRPVDSDVLPK
                     VLGFLMLGLSVWLFFERPKGAAEGEIVEERGVLERWLPVAVTAVAIAVYAGALAWLGF
                     VLASFLLVGGLTWYYGYRRHIVNAIVALAVPLVLYLSMTRLMDIHLPAGILPF"
     gene            2779..4287
                     /locus_tag="VM406_12070"
     CDS             2779..4287
                     /locus_tag="VM406_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HVL76743.1"
                     /translation="MEALESMLYGFGVALQPENLMYVFIGVFAGTIIGMVPGLGPISA
                     LALMIPITYAMNPASGLILMSGVYYGAIFGGSTSSILLNAPGVAGTVATSFDGFPMAK
                     QGKAGKALALAAWASFIGGTVSVLGLMLIAPLLASVAVSFGPAEYFALMLLGLSAVVS
                     LSERSLCKGLIAAVFGVMVSITGIDRQTGTQRYTFDSLHLLQGIDFLVVALGVFALAE
                     VFVMLRAGRDAKTHAPQALGSLKLSKDEVKEISGPVARSSLQGFIVGVLPGAGATIAS
                     FLAYITEKRIAKDGDTFGKGNIKGVAAPEAANNAACGGSFVPLLTLGVPGSGSTAVLL
                     GALLVMGVTPGPLMLEQRPDVFWGVIASMYIGNIFLLILNLPLIPYIARILEVPRPML
                     LALIIVFCMIGVYGLKFSAFDLYLLLAFGLVGLLMRMNGFPLAPMILALILGDLMEEN
                     MRRALQISGGDWATFIEKPISATLLSIALLSLVVPPVIRRIKRVRSTRHRTV"
     gene            4651..6108
                     /locus_tag="VM406_12075"
     CDS             4651..6108
                     /locus_tag="VM406_12075"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HVL76744.1"
                     /translation="MKLNLRKKVLMAALVPAFGLPLAATSFAQATGGQAQQQQQAGQP
                     AQAQQGQQQRQQQAQQIRDMRASEIIGTNVRDQQGENIGNIQDLIVDVNNEEVNYAIV
                     AFGGFLGFGEKLFAYPMDRFQPAGEGNELTLQASEQELRNAPGFDRSNWPTFGMGGYR
                     GEVDKHFGRTAQAGGNLVRMSEFLNTDIQDRAGNDVGQVEDVVVSVTDGQVRYVVVDP
                     DNDLNLGDRLVMLPMNVLEATGEQQAQAMTGEGQQQDRATGAGATGGVGAAGTGPDGQ
                     PRTRGETGAAGTTNTTGTPGGATGASGQQNQQWYQGQDDRPGQPQDRTVASGAGMTGQ
                     GQAGQQQQQAQQQQQDQQQQQDQDLALVLTIEAQQLQQARSFEQNEWPDLNNPSFQRE
                     MENYVASFPQEGQQATGATGQPGGAAAAGRERQETTGAPAQPGRDAGAPGAAQQEGTA
                     AQPPGTQPGRGTAPTGQPAGQQGVPGGATGGTTSN"
     gene            6283..6678
                     /locus_tag="VM406_12080"
     CDS             6283..6678
                     /locus_tag="VM406_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FinO family protein"
                     /protein_id="HVL76745.1"
                     /translation="MLLKELQEKFAPFRDNLPLAIGIDKQLKERLPDVDRKTLRIALG
                     LHTNSTRYLKAMARATQRVDLDGNPAGEVPEAHRTHAEETLRERFRKEAEQRKAQRKV
                     EREAEEAERAAQRRADKLNQLAAKFSRRS"
     gene            complement(6698..6883)
                     /locus_tag="VM406_12085"
     CDS             complement(6698..6883)
                     /locus_tag="VM406_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76746.1"
                     /translation="MPRPDTPFEAASYDLKQFRERRMAERRAVPRAGADRRAPVEHDR
                     RKAANDVPILMMPPVEE"
     gene            complement(7029..7385)
                     /locus_tag="VM406_12090"
     CDS             complement(7029..7385)
                     /locus_tag="VM406_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76747.1"
                     /translation="MQHPIISQVRRIWRLAVPFWLFRDASRGPLPERRANYRYNRAQR
                     KLLPFFMLKWLALGFCLLQAMMPLTTILASLPPGGTAHFLTSLVCMINGIAFAFACVV
                     LTLLSASYVYLSVVED"
     gene            7491..7967
                     /locus_tag="VM406_12095"
     CDS             7491..7967
                     /locus_tag="VM406_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76748.1"
                     /translation="MLSTEQACSRALVTGSVAAAITAAAAAVAGRRQTGSYAAPLNAT
                     SHILWGEDATWRNRATLKYTGTGLLLTHAAGVFWASVYEKLFGRADDTSPPSPLLALA
                     GAAAVSAGAWLTDYHLVPRRFTPGYERRVSDRALAGIFTALAIGLAARDLWRAARS"
     gene            8012..9268
                     /locus_tag="VM406_12100"
     CDS             8012..9268
                     /locus_tag="VM406_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HVL76749.1"
                     /translation="MDNLSHTLSGLVAGELLHRALPAEPSEDRQRRRHRLMLVSCALA
                     SSFPDLDIVLTPLLPEPLGYLLHHRGHTHTVLYAFPQALLLCALLWLLWPSARALLRE
                     SRAARLGLAAGMAVSFGLHLLMDYLNSYGVHPFHPIDSRWLYGDMVFILEPVFWITLG
                     VPLVVLLRRAWLRATLLGLLAALLAYFSAAGFLHWASLIALYAGGLLLIVLQQPVAAA
                     ERAGRLRTPGGARGILAGIALLAAFLAVQAVGARAAARQIGAVLAQKDPDSTLKDSAR
                     TAFPSNPLCWNFVSVESDEQAGHYRLRRGVLSIAPGLLPVSSCPAVLAERHLQREIDP
                     GFALLAEHTASLAELRRLQREDCFFEAWMRFARAPQVEGRVASDKRFSGFGRGNFTTM
                     DLDDFAGRDCPRHVPRWDFPRADLLE"
     gene            9355..10965
                     /locus_tag="VM406_12105"
     CDS             9355..10965
                     /locus_tag="VM406_12105"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVL76750.1"
                     /translation="MSASHPHSIEDRLYILRKRWEQYTADGRFEQFIEFAVAVNSLAE
                     YLNRLRLPGLVRLCEGLENAALMRLGDPSTHPIPRADSLALQRQLDTLIGSVATSRTP
                     ATDRRQPEPAGSPANPEWIKPRTVWLIAADSKRDMADALSRQLQFFGFTVHAQPWAQA
                     ALPDDKPLAVLFVPANGAATEAEFAHIAAVRSHCPASQLVYLGVKSAIEPIVALMRAG
                     IDITIPVDDQPALVLNCVLDLVQTNEQEQYRVLIVEDSRVAVALIRRTLGEHGIDTEA
                     ISDPARLLEVLESYRPDLILMDMHMPRFNGVEATRVLRQMAAYSSLPIVYLSGESDVG
                     MQVEALRLGGDQFLMKPFNPVLLAAVVKTRIERFRETRRSTRLDGLTGLLNHTATKSR
                     LKSMVEQIPDGGALTVAMIDIDHFKSINDTYGHPVGDQVIRGLAWLLKGRLRSFDLIG
                     RYGGEEFMIALPGVGLQQALPVIDRIRADFSALPHAHRGGALHASFSAGVACYPAMQS
                     AAGLTEAADRALLEAKRRGRNRLEAASA"
     gene            11025..11243
                     /locus_tag="VM406_12110"
     CDS             11025..11243
                     /locus_tag="VM406_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76751.1"
                     /translation="MNSKPSRTLTFKCAKCAKPVKVFLQKVSACSHIQPYQGICSCGE
                     IKLHAIGQKDAVESYLASGGAGWSHHHH"
     gene            11283..11849
                     /gene="folE"
                     /locus_tag="VM406_12115"
     CDS             11283..11849
                     /gene="folE"
                     /locus_tag="VM406_12115"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HVL76752.1"
                     /translation="MDDTFSEKDWRRLLRHLGEDPERQGLHETPMRVDKAWQHWTSGY
                     RMDPAEILKVFEDGAEQYNELIVVRGIPVYSHCEHHLAPFFGTATVGYTPNGRIVGLS
                     KLTRLVDCFAKRLQVQERLTIQVAEALMQHVQPLSVGVVVRCRHMCMESRGIQVPGEE
                     TVTSALLGEMRTNLGLRTEFLSLAREKP"
     gene            11896..12252
                     /locus_tag="VM406_12120"
     CDS             11896..12252
                     /locus_tag="VM406_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76753.1"
                     /translation="MAQTLIRMFREIGQAQQAREALLAQGFAADQLHLEASEDEAGAA
                     AGNFVLPRKDSPEGAASGVDRPPGEQDAPANSQAMLRGQYILTIEAGDDAQCARAAEI
                     VDPMGAVDVDRRSAGH"
BASE COUNT         2033 a         4171 c         4065 g         2027 t
ORIGIN      
        1 gcgcgcggcc ggcgtgcagc cgcaggtgcg gctgttcttc accgacatgg gagaggacct
       61 ggtgatcgaa gtggacgaca acggccgcgg ggtgccggac gagctggccg gcgcgatctt
      121 tgacgccggc gtttccacca agggtgccgg gcgcggaatc gggctcgcgc tggtgcgccg
      181 cctgtgccag cagcatggcg gcgacgccac gctcgaggcc agcgagctgg gcggcgcctg
      241 cttcatcgcg gtcgtcgaca agcatgcggt cggcgcgccg tcggacctgg aggtgcgtca
      301 tggctgagcc gtaccgcgtg ctgatcgtcg aggacgattt ccgcatcgcc gacatcaacc
      361 gctccttcgt cgagcaggcc gaggacttca ccgtggtcgg catggcccgc tcgcgcgccg
      421 aggccttggc ttggttcgat gcccatcccg gcggcgtcga cctgctgctg cttgatgtgt
      481 ttcttcccga cgtcgagggc ctcgcgctgt tgtgggaaat ccggcgccgc catcgcgacc
      541 tcgatatcgt gatggtgacg gccgcccgcg aagtggagac ggtggaggag gcgcttcgcg
      601 gcggggtgtt cgatttcctg atcaagccgg tcgaagccac gcgcgtgcag aaaatgctcg
      661 cgcgctttcg cagccggcgc cagctggccg acggccggcg cgagctgagc caggaacagc
      721 tcgaccacgc gctgtcttcc ctgtacgcaa gccccggtgc gtcggcgcgg cgcgcgctgc
      781 ccaagggcat cgatgcgctc acgctcgaca ccgtgctcga ggtgctgcgc cagtgcgagg
      841 gggcgctgac cgccactgag ctcgcgggcc gcatcggcag cagccgctcg accgcgcgcc
      901 gctacctcga gcacctggtc ggcgagcaac tggtgagcgc cgaacaaagc tatggcgatg
      961 tcgggcgccc gcagcgccag taccggctgc gcacccgccg gggcgcggcc tgaccatgaa
     1021 caaaatgcac taaatgcacg cagcgcactt tattcaatca tttggttcaa atctatccat
     1081 gccgcggcaa tcttcctaag atggaatctg gccgcggcgc gactgcgacg gcggccctgc
     1141 atgctgccgc ggcattgtct ggcccgcggc gcaaacagcc caaccgggcg atgatccaag
     1201 agaaggagac atgatgttca atccgcgagt acttccgtgc atgcgtgcgc tcatgcgcgg
     1261 caccgccgcc gccatgctgc tcggcgccgc cgccgttccc gcgctggccc aggactggca
     1321 gccgacccga cctatcgaat acatcgcccc cgccaatccc ggcggcggtt gggacacgct
     1381 ggtccgcacc accgcgcgcg tgatccagga ggaaaaactc gcgccgcaga attttgcgcc
     1441 gatcaatatt cccggcggcg gaggcgcggt cgcgtgggcg cagatcgccg cgcagaaggg
     1501 taatccgcat cgcctgttcg cggccagccc gccgatcatc ctggtgccgc tgtcgggagc
     1561 ctcgcgcttc acgcacaagg acttcacgcc gatcgcgcgg ctgatcactg actactcgat
     1621 cctgctggtg cgcgcggact cgccgtacaa gacagccaat gatctgttca acgcgatcaa
     1681 ggagcgcccg ggactgtcgg tcggcggcgg atcggccccc ggttcgatgg accacatctc
     1741 gatggcgggc gcggccagcg cggccggcct gaagcccgca caggtcaact acatcgcgtt
     1801 ctccggcggc ggcgaggcga tgaccaacct gctcggcggc catgtggcgg cggtgatcac
     1861 cggcgccggc gaggcgcagg cgcaactgaa gggcggccag atccgcgcgc tcgcggtgtc
     1921 ttcgcccaag cgcatcgcat cgctgccgaa tgtgccgacc taccaggagc agggcatcaa
     1981 ctacaccttc gacatctggc gcggcatcat gggcccgccg aacatgccgc cggaagccgt
     2041 gcgctactac gagaacatgt atgcgcgcat gctcaagacc gacggctggg cgaaggcgcg
     2101 cgaccagctc ggctggatcg atgcctacca gaacagcgcc gagttcggca agttcctcga
     2161 cgcccagcac gcgcagttca gctccgtgtt gcaagacctc ggcctgctgc gccgctaagc
     2221 gtcccggccc ggccccgggc cgccaagcgc gccccggggc cgtcgcacgc ctcggcgcgc
     2281 gtcgcgctgg agaccccgat gaaacgactg aacatcaacc aggtgctggc gatcctgatc
     2341 gcggccctgt ccttcgccta tctgttcgag gcgtaccgca ttcccacctt cccgatcccg
     2401 cggccggtcg attccgacgt gcttcccaag gtcctcggct tcctgatgct gggcctgtcg
     2461 gtctggctgt tcttcgagcg ccccaagggc gcggccgagg gcgaaatcgt cgaggagcgc
     2521 ggcgtactcg agcgctggct gccggtggcg gtgacggcgg tcgcaatcgc cgtctacgcc
     2581 ggcgcgctcg cgtggctcgg tttcgtgctc gcctcgttcc tgctggtggg cgggctcacc
     2641 tggtattacg gctaccggcg ccacatcgtc aacgcgatcg tcgcgctggc cgtgccgctg
     2701 gtcctgtatc tgagcatgac gcggctgatg gacatccatc tgccggccgg catcctgccg
     2761 ttctgagccg aggcgaacat ggaagcactt gaaagcatgc tttacggctt cggcgtcgcg
     2821 ctgcagccgg aaaacctgat gtacgtgttc atcggcgtgt tcgccggcac catcatcggc
     2881 atggtgcccg ggctggggcc gatctcggcg ctggcgctga tgatcccgat cacgtacgcg
     2941 atgaacccgg cctcgggcct gatcctgatg tccggggtgt actacggcgc tatcttcggc
     3001 ggctccactt catcgatcct gcttaacgcg ccgggggtgg ccggcacggt tgcgacttcg
     3061 ttcgacggct ttccgatggc caagcagggc aaggccggca aggcgctcgc gctggctgca
     3121 tgggcatcgt tcatcggcgg aaccgtgagc gtgctcggtc tgatgctgat cgccccgctg
     3181 cttgccagcg tggcggtgtc cttcggcccg gccgagtatt tcgcgctgat gctgctggga
     3241 ctgagcgccg tggtcagcct gtccgagcgc tcgctgtgca agggcctgat cgccgcggta
     3301 ttcggggtga tggtgtccat caccggcatc gaccgccaga ccggcacgca gcgctacacc
     3361 ttcgattcgc tgcacctgtt gcagggcatc gacttcctgg tggtggcgct cggcgtgttc
     3421 gcgctggcgg aggtgttcgt gatgctgcgc gccggacgcg acgccaagac ccatgcgccg
     3481 caggcgctcg gctcgctcaa gctctcgaag gacgaagtca aggagatttc gggaccggtc
     3541 gcgcgcagct cgctgcaggg cttcatcgtc ggcgtactgc ccggcgccgg cgcgacgatc
     3601 gcctccttcc tcgcctacat cactgaaaag cgcatcgcca aggacggcga taccttcggc
     3661 aagggcaaca tcaagggcgt ggccgcaccc gaagccgcca acaacgccgc ctgtggcggc
     3721 tccttcgtgc cgctgctcac gctcggcgtg cccggctcgg gctcgaccgc ggtgctgctg
     3781 ggcgcgctgc tggtgatggg cgtcacgccc ggtccgctga tgctggagca gcggcccgac
     3841 gtgttctggg gcgtgattgc gagcatgtat atcggcaaca tcttcctgct gatcctcaac
     3901 ctgccgctga ttccatacat cgcgcgcatc ctcgaagtgc cgcgtccgat gttgctcgcg
     3961 ctgatcatcg tgttctgcat gatcggcgtc tacggcctca agttcagcgc cttcgatctc
     4021 tacctgctgc tcgccttcgg cctcgtcggc ctcctaatgc gcatgaacgg ctttcccctt
     4081 gcgccgatga ttcttgcgct catcctcggc gacctgatgg aggagaacat gcgccgcgcc
     4141 ctgcagatct ccggcgggga ctgggcgacc ttcatcgaga agcccatctc ggccacgctg
     4201 ctgtcgattg ccttgctctc gctggtcgtg ccgccggtga ttcgtcgcat caagcgcgta
     4261 cgttccacgc ggcaccggac cgtgtgacaa atctccccgg cacggcgcgc gctgcgcggt
     4321 gccgggaagc gcggccgccg aagttggtaa aaacttcatg ccgcttcccg tcctttccgc
     4381 cgcccttctg tcccaccgaa ccagcccgcc gcccggccag gccgcggcgc gctcccgcgc
     4441 ggcggaatcc cggccggttt tcctccagtg tcgggaagtc cgccgatctt tcctgtgttt
     4501 gcgccgtgtt gctcccatac ggccggaccg gcccggaact tgctcaagtc ttcacatccg
     4561 ggcgatgccg cagggcctcg cttgctctct ttttttgcga gagcaggtat gggcggcggt
     4621 atctcggcaa ccagaaaaca ggagcaagcc atgaaattga accttagaaa gaaagtattg
     4681 atggcggcgc tggtgccggc attcggtctg ccgttggcgg caacctcgtt cgcgcaggcg
     4741 acaggcggcc aggcccagca acagcagcaa gccgggcagc ccgcccaggc ccagcagggg
     4801 caacagcaaa ggcagcagca ggcgcagcag atccgcgaca tgcgtgccag cgaaatcatc
     4861 ggcacgaacg tgcgcgacca gcagggcgaa aacatcggca acatccagga cctgatcgtc
     4921 gatgtgaaca atgaggaagt gaactacgcg atcgtcgcct tcggcggctt ccttggcttc
     4981 ggggaaaagc tgttcgcgta tccgatggat cgcttccagc cggcaggcga aggcaatgag
     5041 ttgacgctgc aggcaagcga gcaggaactg cgcaacgccc cgggcttcga tcgttcgaac
     5101 tggccgacat tcggcatggg cggctatcgc ggcgaggttg acaagcactt cggccgcacc
     5161 gcacaggccg gcggcaatct cgtgcggatg agcgagttcc tgaacaccga catccaggac
     5221 cgcgccggca atgacgttgg ccaggtggaa gacgtggtcg tcagtgtcac cgacggacaa
     5281 gtgcgctatg tcgtggtcga tcccgacaat gacctgaacc tcggtgatcg actcgtcatg
     5341 ctgccgatga atgtgctgga agcgaccggc gaacagcagg cgcaggcgat gacgggtgag
     5401 ggccagcagc aggatcgtgc gaccggcgcg ggcgcgaccg gtggtgtcgg cgctgcgggt
     5461 accggcccgg atggtcagcc gcgcacgcgc ggggagaccg gcgcggccgg cacgaccaac
     5521 acgaccggca cgccaggcgg cgcgacaggc gccagcggcc agcagaacca gcagtggtat
     5581 caggggcagg atgaccgtcc tggccagccg caggatcgca cggtcgccag cggcgcaggc
     5641 atgacgggac aggggcaggc tggtcagcag cagcaacagg ctcagcaaca gcagcaggat
     5701 cagcaacagc agcaggatca ggacctcgca ctggtactga ccatcgaagc gcagcaacta
     5761 cagcaggccc gctcgttcga acagaatgag tggcccgacc tgaacaaccc gtcgttccag
     5821 cgcgagatgg agaactatgt tgcgtccttc ccgcaggaag ggcagcaggc gaccggcgcg
     5881 accggccagc ccggcggtgc ggcagccgct ggccgcgagc gccaggagac gaccggcgcg
     5941 ccagcccagc cgggccggga tgccggtgcc ccgggcgccg cgcagcaaga gggtaccgcc
     6001 gcccagccgc cgggcactca gcccggccgt ggcacggctc cgaccggtca gccggccggc
     6061 cagcagggcg tgccgggtgg cgccaccggc gggaccacgt caaactgagg gaggcctcgc
     6121 ccatggaaac tgcgcacttc ggtgcgcagt tttcattttc tccgcgtcgg ccccgcgcaa
     6181 cgcgtaaagg ggccggcccg tgatatgctc cggcatcctc aacttcctcc gtcggcgatg
     6241 agctccccag caaccactcc ttcccccgta cagaacgcaa gggtgctgct caaggaactg
     6301 caggagaagt tcgccccgtt tcgcgacaat cttccgcttg ccatcggcat tgacaagcag
     6361 ttgaaggagc gcctgcccga cgttgatcgc aagaccctgc gcatcgcact cggactgcat
     6421 acgaactcca cccggtatct gaaggcgatg gcgcgtgcga cccagcgcgt ggacctcgac
     6481 ggcaatccgg ccggagaggt gcccgaggcg catcgtacgc acgcggagga aaccctgcgc
     6541 gaacgcttcc gcaaggaagc tgagcagcgc aaggcgcaac gcaaggtcga gcgggaagcg
     6601 gaagaagcgg aacgcgcggc gcagcgccgc gccgacaaac tcaatcagct cgcggcgaag
     6661 ttctcgcggc gcagctgacc atctatttcg ccaccggcta ttcctccacc ggcggcatca
     6721 tcaggatcgg cacatcgttg gccgccttgc ggcggtcatg ctcgacgggc gcgcgccggt
     6781 cggcaccggc acgcgggacg gccctgcgct ccgccatacg gcgctcgcga aactgcttca
     6841 gatcgtagct ggccgcttcg aatggggtgt cggggcgtgg cattggggca tcctcggtcg
     6901 agtaacttcg ttctcttaac acttccggac gcctagtcta cgcaagggcc gcaagcagca
     6961 cttgagtttg cacaagcgtc cggcatgcgc cgggcgcccc gatgcgctga tccgcacggc
     7021 gcgcctgttc aatcctcgac cacgctcaga tacacatagc tcgctgaaag cagcgtcagc
     7081 accacgcacg caaaagcgaa cgcgatcccg ttgatcatgc agaccaggct ggtgaggaag
     7141 tgcgcggtgc cgccgggcgg cagactggcc aggatcgtgg tcaagggcat catcgcctgc
     7201 agcaggcaga agccaagcgc gagccacttg agcatgaaga acggcagaag cttgcgctgc
     7261 gcgcggttgt agcggtagtt cgcacgcctt tcgggcaggg ggccgcggct ggcgtcgcgg
     7321 aagagccaga acgggacggc cagccgccag atccggcgga cctgggaaat tattggatgt
     7381 tgcaaggtca gacagatgta aatggtttgg caacagcgcg tcgctttgct gcggcaaacc
     7441 cgttttcagt cgaccagatt tctagcaaac cgtgttccaa ggagaagccg atgctcagta
     7501 ccgaacaggc atgcagccgc gcgctggtaa cgggaagcgt ggccgccgcg atcaccgccg
     7561 ccgcagccgc cgtggcagga cgccgtcaga ccgggtccta tgccgccccc ctgaacgcaa
     7621 ccagccacat cctgtggggc gaggatgcga catggcgcaa ccgtgcaacc ctgaaataca
     7681 ccggaaccgg gctgctgctc acgcacgcgg ccggcgtgtt ctgggcgagc gtctatgaga
     7741 agctgttcgg gcgtgcggat gacacgtctc cgccgtcgcc gctgctggcg ctggcgggag
     7801 ccgcggcggt cagcgccggc gcctggctga cggactacca cctggtcccg cgccgcttca
     7861 cccccggcta cgagcggcgc gtctcggacc gcgcactggc aggaattttc accgcgcttg
     7921 cgattgggct cgctgcccgc gacctatggc gggccgcccg ctcctgacgc gtcgatcagt
     7981 ccggctttga gtttgccggt accattgccg gatggataac ctcagtcata cgctgtccgg
     8041 cctggtcgcc ggcgaacttc tgcaccgtgc gctgcctgcc gagccgagcg aggatcgcca
     8101 gcgccggcgc catcggctga tgctggtttc gtgcgcactc gcgagcagct tcccggatct
     8161 cgacatcgtt ctcactccgt tgctgccgga gccgctcggc tacctgctgc atcatcgcgg
     8221 gcatacccat accgtgctct acgcgttccc gcaggcgctg ctgctgtgcg cgttgctgtg
     8281 gctgctgtgg ccgtcggcgc gcgcactgtt gcgcgagagc cgggccgcgc ggctcgggct
     8341 ggctgccggc atggccgtga gcttcggcct gcatctgctg atggattacc tcaattccta
     8401 cggcgtgcat ccgttccatc cgatcgactc acgctggctg tacggcgaca tggtgttcat
     8461 cctggagccg gtgttctgga tcacgctcgg cgtgccgctg gtcgtgctgc tgcgtcgcgc
     8521 ctggttgcgc gcgaccctgc tcgggctgct cgcggccctg ctcgcctatt tctcggcggc
     8581 cggcttcctg cactgggctt cgctgatcgc cttgtatgcg ggcggcctgc tgctgatcgt
     8641 gctgcagcag cccgtcgcgg cggccgagcg tgcgggacgc ctgcgcacgc cggggggcgc
     8701 gcgcggcatc ctggccggca ttgcactgct cgcggcattc ctcgccgtgc aggcggtcgg
     8761 cgcgcgcgcc gccgctcggc agatcggcgc ggtgctcgcc cagaaggacc cggacagcac
     8821 gctgaaagac agcgcgcgca ctgcattccc gagcaatccg ctgtgctgga acttcgtctc
     8881 cgtcgaaagc gacgagcagg ccggccacta tcggctgcgc cgcggcgtgc tgtcgatcgc
     8941 gcccggcctg ctgccggtga gctcctgccc ggcggtgctg gccgagcgcc acctgcagcg
     9001 cgagatcgac cccggcttcg cgctgctggc cgagcacacg gcaagcctgg cggaactgcg
     9061 ccggctgcaa cgcgaggatt gcttcttcga agcctggatg cgctttgcgc gcgcgccgca
     9121 ggtggaggga agggtggctt ccgacaaacg cttctctggc ttcggtcgag gcaatttcac
     9181 cacgatggat ctcgacgatt tcgccggccg cgactgtccg cgccatgtgc cgcgctggga
     9241 tttcccacgg gcggacctgc tcgaatagca ccgtcgtggt gcgccgcaca tcgattgcgt
     9301 gttgccgctt ggacatcgat ccgcgataat gctccatcgt ccgccggccc agccatgagc
     9361 gcttcccatc cccattcgat cgaagaccgc ctgtacatcc tgcgcaagcg ctgggaacag
     9421 tacacggcgg acggtcgctt cgagcagttc atcgagttcg cggtggcggt caacagcctg
     9481 gccgagtacc tgaaccgcct gcgcctgccg ggcctggttc ggctgtgcga aggacttgag
     9541 aacgcggcgc tgatgcgcct gggcgacccg tccacccatc cgatcccgcg cgccgacagc
     9601 ctcgcgctgc agcgccagct cgacaccttg atcggctcgg tcgccacctc gcgcacccct
     9661 gccactgacc gccgccagcc cgagccggcg gggagccctg ctaacccgga atggatcaag
     9721 ccgcgcacgg tctggctgat cgccgccgac agcaagcgcg acatggccga cgcgctgagc
     9781 cgccagctgc agttcttcgg cttcacggtg catgcgcagc cctgggcgca ggccgcgctg
     9841 ccggacgaca agccgcttgc cgtgctgttc gtgccggcca atggcgcagc caccgaggcc
     9901 gagttcgccc atatcgcggc ggtgcgcagc cattgtccgg ccagccagct ggtctacctc
     9961 ggcgtgaaat cggcgatcga accgatcgtc gcactcatgc gcgccggcat cgacatcacg
    10021 attccagtcg acgaccagcc cgcgctggtg ctcaactgcg tgctcgacct cgtccagacc
    10081 aacgagcagg aacaataccg tgtgctgatc gtcgaggatt ctcgcgtggc ggtcgcgctg
    10141 atccggcgca cgctcggcga gcacggcatc gacaccgaag ccatcagcga cccggcccgc
    10201 ctgctggagg tgctggagag ttaccggccg gacctgatcc tgatggacat gcacatgccg
    10261 cgcttcaacg gcgtcgaagc cacgcgcgtg ctgcgccaga tggccgccta cagctccctg
    10321 cccatcgtgt acctgtccgg cgaatccgac gtcggcatgc aggtcgaggc gttgcgcctg
    10381 ggcggcgacc aattcctgat gaagcccttc aacccggttc ttctggccgc ggtggtcaag
    10441 acgcgcatcg agcgtttccg cgagacgcgc cgctcgaccc ggctcgacgg cctgaccggc
    10501 cttctgaacc acaccgccac caagtcgcgc ctgaagagca tggtcgaaca gattcccgac
    10561 ggcggcgcgc tgacggtcgc gatgatcgac atcgaccatt tcaagtcgat taacgacacc
    10621 tacggccacc cggtcggcga ccaggtgatc cgcggcctgg cctggctgct gaaggggcgg
    10681 ctgcgctcgt tcgacctgat cggccgctac gggggcgagg aattcatgat tgcgctgccg
    10741 ggcgtggggc tgcagcaggc gctgccggtg atcgaccgca tccgcgccga cttcagcgcg
    10801 ctgccgcacg cacatcgcgg tggcgccctc cacgccagct tcagcgccgg cgtcgcctgc
    10861 tatccggcga tgcaaagcgc ggccggtctc accgaggcgg cggaccgcgc gctcctcgaa
    10921 gccaagcgcc gcggccgcaa ccggctcgag gccgcttcag cctgaggcgc ttgcgggcac
    10981 ggcgtcccga tacgcgcgat aatgcgcgtt tttgcgaggg acagatgaac agcaaaccgt
    11041 cgcgcacgct cacattcaag tgcgccaagt gcgccaaacc ggtcaaggtc ttcctgcaga
    11101 aggtatcggc ctgctcccac atccagccct atcagggcat ctgcagctgc ggcgagatca
    11161 agctgcatgc gatcggccag aaagatgcgg tggagtccta cctggcctcc ggcggcgctg
    11221 gctggtccca tcatcatcac tgaggcggtg gccatcttca gggagccgca aggattacga
    11281 caatggacga cacattttcc gaaaaggact ggcggcgcct gctgcgccat ctgggagagg
    11341 acccggagcg ccagggcctg catgaaacgc cgatgcgggt cgacaaggcc tggcagcact
    11401 ggacttccgg ttaccgcatg gacccggccg agattctgaa ggtattcgaa gacggcgccg
    11461 agcagtacaa cgaactgatc gtggtgcgcg gcataccggt gtacagccat tgcgaacacc
    11521 atctggcgcc gttcttcggc accgccaccg tcggctatac gcccaacggc cgcatcgtgg
    11581 gcctgtcgaa gctgacccgg ctcgtcgact gcttcgccaa gcggctgcag gtgcaggagc
    11641 ggctcacgat ccaggtcgcc gaagcgctga tgcagcatgt gcagccgctg tcggtcggcg
    11701 tggtcgtgcg ttgccgtcac atgtgcatgg aaagccgcgg catccaggtg ccgggcgaag
    11761 aaacggtgac ctcggcactg ctcggggaaa tgcgcaccaa cctcggcctg cgcaccgaat
    11821 tcctgtcgct ggcgcgcgag aaaccctgag cggccgcgat ggtctgaccc ctgaatactg
    11881 aacgggagaa agaccatggc gcaaaccctg atccggatgt tccgcgaaat cggccaggcc
    11941 cagcaggcgc gcgaggcgct gctggcgcaa ggcttcgcag ccgaccagct ccacctggag
    12001 gcgtccgagg acgaggcggg cgcggccgcc ggcaacttcg tacttccacg caaggattcg
    12061 cccgaaggcg cggcatccgg cgtcgatcgt ccgcccggcg agcaggatgc gcccgccaat
    12121 tcgcaggcga tgctgcgcgg acaatacatc ctgacgatcg aagccggcga cgacgcgcag
    12181 tgcgcgcgcg cagccgagat tgtcgatcca atgggtgcgg tcgacgtcga ccggcgcagc
    12241 gctggtcact agagcactta ggcgcgcgcc ggcgcgcgcc taagtgctct agtgac
//
LOCUS       DATLGF010000395         5148 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_183134, whole genome shotgun sequence.
ACCESSION   DATLGF010000395 DATLGF010000000
VERSION     DATLGF010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5148
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183134"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VM406_12125"
     CDS             <1..234
                     /locus_tag="VM406_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76754.1"
                     /translation="TFFSVMFDFTFTTPGRRSNCFIMNSEKEFMMKQLLRLPGVVNVK
                     SNITLKKVKSTHVLPLDHLTQPAKSARRMVLTR"
     gene            complement(407..1378)
                     /locus_tag="VM406_12130"
     CDS             complement(407..1378)
                     /locus_tag="VM406_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVL76755.1"
                     /translation="MSIISKKRRTVAALLAVCLTAPAITVQAQQQIRMIVANAAGSSV
                     DSVARTISDPLSKALGQAIFVENIPGAGGTLGSAQLARAPKDGNTLGMVSNNHVVNPS
                     LYKSMSYDTLRDFAPVTIVAGSPIVLVAHPSVPANNLKELIALAKSKPGEVNYGSSGN
                     GTILHLTGEALAHQAGIKLQHIPYKGTGQLTSDVLGGQIQLAFLGVTGAAPHIKSGRL
                     KALGVTTAARSTLLPDVAPFAEQGLPNYNMQGWIALIAPAGVPDATVKKLYSTTKNLL
                     ATPEFNAALESQGFTPMGTTPEATLGIFRNEMAQAAELVRQSGMKID"
     gene            complement(1496..2020)
                     /locus_tag="VM406_12135"
     CDS             complement(1496..2020)
                     /locus_tag="VM406_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HVL76756.1"
                     /translation="MATYRLGQRAPQIDETAFVSAEATLIGSVQLQRDTSVWPGAVLR
                     GDNEPIVIGAGSNVQEGAVLHADPGFALVVENNVTIGHQAMLHGCTVKEGALIGIQAV
                     ILNGAVIGRNCLVAAGAVVTEGKVFPDNSLIVGAPAKLLREMSEEGITAMHRNAAAYR
                     ERAAYYKENLVRVD"
     gene            complement(2029..3240)
                     /locus_tag="VM406_12140"
     CDS             complement(2029..3240)
                     /locus_tag="VM406_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HVL76757.1"
                     /translation="MSTEDMNQAGGERLPLAGIRVVEVTHMVMGPTCGMILGDLGAEV
                     IKVEPPKGDNTRRLLGAGAGFFRTFNRNKKSVALDTETEEGREALRKLVDTADVFIEN
                     FKPGRMKSLGLDYETLKKRNPKLIYVSHKGFLAGPYENRLALDEVVQMMAGLAYMTGP
                     VGRPLRAGSSVNDIMGGMFGAIGVLAALNERWKSGVGHEVQSALFENCVLLSAQHMQQ
                     YVVTGEAAAPMPERISAWAVYDVFEMANGEQLFVAATGDAQWRALCGLIGREDLLADS
                     RLATNNDRVVARPWLLATLRETLRQIDAKALAAQLEANNVPFASIVRPEQLFDDPHLR
                     QSGGLVPLTLEDGSQTDMPLLPISLDGQRLKPRRPIPKIGEDTREVLLALGYTEAQIA
                     SLQHAAPKAAA"
     gene            complement(3237..4187)
                     /locus_tag="VM406_12145"
     CDS             complement(3237..4187)
                     /locus_tag="VM406_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HVL76758.1"
                     /translation="MANSQIDVVVQEVGLRDGLQSIAATMPTADKKCWIDAEYAAGVR
                     HMEVASFVPARLLPQMADADEVIAHALTYSDLTVTALVPNLKGAEKALASGVHRIVAP
                     ISVSAAHSMANVRKTPMDMIEEFRRIRELRDATRPGTVLIAGLSTVFGCTLQGDVPLA
                     DVRDVVLRSLQAGADIVALADTTGHATPGQVDRFFSALRPLSPEKLSVAHFHDTRGMA
                     LPNTMVALQHGIREFDASLAGIGGCPHAPGATGNVATEDLVFMLESLGYRTGIDVAQL
                     LAAREIIRTVLPDASLYGALARAGLPKGYRPAHQIEKAVA"
     gene            complement(4411..>5148)
                     /locus_tag="VM406_12150"
     CDS             complement(4411..>5148)
                     /locus_tag="VM406_12150"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVL76759.1"
                     /translation="TPTPAGEALLAHARQLLRGAEKMQGELSEYATGVRGHIHIYASV
                     SAIAEFLPDDISAFLAEHGNIRVDMEERVSSQVLRTVEENSAAIGVCWDLADTRDLQV
                     IPYKRDHLTAVVHPDHPLARRRKVSFEEILDYDLIGQAPESMMNLFLERMAAKQGRSV
                     RNRMHVASFEAVYRIVRANLGMSIVPAEAIQPYQATLGLRVIPLSDSWAHRRFIVCMR
                     DYDALPLPSRLFVDFLRTDQAGKDERD"
BASE COUNT          817 a         1678 c         1700 g          953 t
ORIGIN      
        1 accttcttca gcgtgatgtt cgacttcacg ttcaccaccc ccggcaggcg cagcaactgc
       61 ttcatcatga actccgagaa ggagttcatg atgaagcagt tgctgcgcct gccgggggtg
      121 gtgaacgtga agtcgaacat cacgctgaag aaggtcaaga gcacgcatgt gctgccgctc
      181 gaccatctga cgcagccggc gaagtctgca cggcggatgg tgttgacgcg gtgaagaggg
      241 ttgccgtcta gcttggcctg aatggcttcg tcactgcggc cttgcacccg gttcgtcgcc
      301 ccggcgaaag ccggggccca gtgacttctg cgctacgtgc tgaacgacac tgggtcccgg
      361 ctttcgccgg gacgacggtt ggagggatcc gcgtaacacg cagctctcag tcgatcttca
      421 tccccgactg cctgaccagc tcggccgcct gcgccatctc gttgcggaag atgcccagcg
      481 tcgcctcggg cgtggtgccc atcggcgtga agccctggga ctcgagcgcg gcgttgaatt
      541 ccggcgtggc cagcaggttc ttcgtggtcg agtacagctt cttgaccgtc gcatccggca
      601 ccccggccgg cgcgatcagt gcgatccagc cctgcatgtt gtagttcggc aggccttgct
      661 cggcaaacgg ggccacatcc ggcagcagcg tcgagcgcgc agcagtcgtc acgccgagcg
      721 ctttcagccg gcccgacttg atgtgcggcg cggcgccggt cacgccaagg aaggcaagct
      781 ggatctgacc gccgagcaca tcgctggtga gctgaccggt tcccttgtac ggaatgtgct
      841 gcagcttgat gcctgcctga tgcgcgagcg cttcgccggt cagatgcagg atggtgccgt
      901 tgcccgagga gccgtagttg acctcgcccg gcttggactt ggccagcgcg atcagctcct
      961 tcaggttgtt ggcgggtacc gagggatgcg cgaccagcac gatcggcgag ccggcgacga
     1021 tcgtcaccgg cgcgaaatcg cggagcgtgt cgtacgacat cgacttgtaa agactgggat
     1081 tgacgacatg gttgttcgac accatgccga gcgtattgcc gtccttcggc gcgcgcgcga
     1141 gttgcgcgct gcccagcgtg ccgccggcgc cgggaatgtt ctcgacaaag atcgcctggc
     1201 ccagggcctt ggacagcgga tcgctgatcg tccgcgcgac cgagtcgacg cttgaacccg
     1261 ccgcgttggc gacgatcatg cggatctgct gctgggcctg cacggtgatg gccggtgcgg
     1321 tcaggcagac ggccagcaat gcggcgacgg tgcgccgctt cttggaaatg atgctcatcg
     1381 ttgtctccat ccgatcatgc ttgggactgt cgtcgcagcc ccggtttgaa aaactgttgc
     1441 ggaactggcc gggacgcgcc gttcgttgtt ggtcttgcgc gcccgggtcc tccgttcaat
     1501 caacccgtac caggttctcc ttgtagtacg cggcgcgctc cctgtacgcg gccgcattgc
     1561 ggtgcattgc ggtgattccc tcttcgctca tctcgcgcag cagcttggcc ggcgcgccga
     1621 cgatcagcga gttgtcgggg aataccttgc cttcggtgac cactgcgccg gccgccacca
     1681 ggcaattgcg cccgatcacc gcgccgttca ggatcaccgc ctgtatgccg atcagcgcgc
     1741 cttccttcac cgtgcagcca tgcagcatcg cctggtggcc gatcgtcacg ttgttctcca
     1801 ccaccagcgc gaagccggga tcggcatgca gcaccgcgcc ttcctgcaca ttgctgcccg
     1861 cgccgatcac gatcggttcg ttatcgccgc gcagcacggc gccgggccac acgctggtat
     1921 cgcgctgcag ctgcacgctg ccgatcagcg tcgcctcggc ggagacgaag gccgtctcgt
     1981 cgatctgcgg ggcgcgttgc ccgagccggt atgtcgccat gctgccccct acgccgccgc
     2041 cttgggcgca gcgtgttgca ggctggcgat ctgtgcttcg gtgtagccga gtgcgagcag
     2101 cacctcgcgc gtgtcctcgc cgatcttcgg aatcggacgg cgcggcttca ggcgctgtcc
     2161 atccagtgag atcggcagca gcggcatatc ggtctgcgag ccatcctcca gcgtcagcgg
     2221 caccaggccg ccgctttgcc gcaagtgcgg atcgtcaaac agctgctccg ggcgcacgat
     2281 ggacgcgaac gggacattgt tcgcctcgag ttgcgccgcg agcgccttcg catcgatctg
     2341 ccgcagcgtt tcgcgcagcg tggcgagcag ccaggggcgc gcgaccacgc ggtcgttgtt
     2401 cgtcgccagg cgggaatcgg caagcagatc ctcgcgaccg atcaggccgc acagcgcgcg
     2461 ccattgcgcg tcgccggtcg cggcaacgaa cagctgctcg ccgttcgcca tctcaaacac
     2521 gtcatacacg gcccatgcgc tgatgcgttc cggcatcggc gctgccgcct cgccggtcac
     2581 cacgtactgc tgcatgtgct gcgccgagag cagcacgcag ttctcgaaca gcgcgctctg
     2641 cacttcgtgg ccgacgccgc tcttccagcg ttcgttcaac gcggccagca cgccgatcgc
     2701 gccgaacatg ccgcccatga tgtcgttgac cgaacttccc gcacgcagcg ggcggccgac
     2761 cgggcccgtc atgtaggcca ggccggccat catctgcacc acttcgtcga gcgcgaggcg
     2821 gttttcgtac gggccggcga ggaaaccctt gtgcgagacg tagatcagct tcggattgcg
     2881 cttcttcagc gtctcgtagt ccaggccgag gctcttcatg cggcccggct tgaagttctc
     2941 gatgaacacg tcggcggtgt cgaccagctt gcgcagcgct tcgcggccct cttcggtctc
     3001 ggtgtcgagc gcgacgctct tcttgttgcg gttgaaggta cggaagaagc cggcgccggc
     3061 gccgagcagg cggcgcgtgt tgtcgccctt gggcggctcg accttgatca cctcggcgcc
     3121 gaggtcgcca aggatcatgc cgcaggtcgg ccccatcacc atgtgcgtga cctcgaccac
     3181 gcgaatgccc gcgagcggca ggcgttcgcc gccggcttga ttcatgtctt cggtgctcat
     3241 gcaacggcct tttcgatctg atgtgcgggg cggtagccct ttggaagccc ggcgcgggcc
     3301 agcgcgccat acaggctggc gtccggcagc acggtgcgga tgatctcgcg cgcggcgagc
     3361 agctgtgcta catcgatgcc ggtgcggtag cccaggctct ccagcatgaa caccaggtcc
     3421 tccgtggcga cattgccggt ggcgcccggc gcgtgcggac agccgccgat gccggcaagc
     3481 gaggcatcga attcgcggat gccgtgctgg agtgcaacca tcgtgttcgg cagcgccatg
     3541 ccgcgtgtgt cgtgaaaatg cgcgacgctg agcttctcgg gcgacaatgg ccgcaacgcg
     3601 gagaagaagc ggtccacctg gccgggtgtg gcatgtccgg tggtatcggc cagcgccacg
     3661 atgtcggcac cggcttgcag cgaacgcagc acgacgtcgc gcacgtcggc aagcgggaca
     3721 tcgccctgta aggtgcagcc gaacacggtg gacaggccgg cgatcagcac cgtgccgggc
     3781 cgggtcgcat cgcgcaattc gcggatgcgg cggaattcct cgatcatgtc catcggcgtc
     3841 ttgcgcacgt tggccatgct gtgcgcggcg ctgaccgaga tcggcgcgac gatccggtgc
     3901 acgccggatg cgagcgcctt ttcagcgccc ttcaggttcg gcaccagcgc ggtgacggtg
     3961 aggtcggaat aggtgagcgc gtgggcgatg acttcgtccg catcggccat ctgcggcagc
     4021 agccgcgcgg ggacgaagga cgccacttcc atgtggcgca cgccggccgc gtattccgcg
     4081 tcgatccagc acttcttgtc ggcggtgggc atcgttgcgg caatgctttg caggccgtcg
     4141 cgcaggccga cttcctgcac gaccacgtcg atctggctgt tcgccatgtc tcttccttca
     4201 ttctgccggg cgccatggcg cctcgttcag cgaaagaata gccatcgcgg gaaggaagct
     4261 gaagcggtat ttcggaaggg gatccatcgt gaaacgagat ggatgtgagg agaggaaagt
     4321 tacagggttt gggggattga gcgatggcgg gcacggtgga tgacacagca tgggatgaca
     4381 tcccggctag cagagtaccg ccagcccggt tcaatcgcgt tcgtctttcc ccgcctggtc
     4441 cgtgcgcagg aaatccacga acaaccgcga cggcagcggc aacgcgtcat agtcgcgcat
     4501 gcagacgatg aagcgccggt gcgcccatga atcggacaac gggatcacgc gcagcccgag
     4561 cgtggcctga tacggctgga tcgcctcggc cggcacgatg ctcatgccga ggttcgcgcg
     4621 cacgatgcgg tacaccgctt cgaagctggc cacatgcatg cggttgcgca cgctgcgccc
     4681 ctgcttggcc gccatgcgtt cgaggaacag gttcatcatg ctttccggcg cctggccgat
     4741 caggtcgtaa tccaggatct cctcgaacga caccttcctg cgccgcgcca gcggatgatc
     4801 cggatggacc accgccgtca ggtgatcgcg cttgtacgga atgacctgca ggtcacgggt
     4861 atcggcgaga tcccagcaga cgccgatggc cgcgctgttc tcttccaccg tgcgtagcac
     4921 ctggctgctc acccgctctt ccatgtcgac gcggatattg ccgtgttcgg caaggaaggc
     4981 gctgatgtcg tcgggcagga attcggcgat cgccgagacg ctcgcataga tgtggatatg
     5041 gccgcgcacg cccgttgcgt actcgctcag ctcgccctgc atcttctccg cgccgcgcag
     5101 cagctggcgc gcatgggcaa gcagggcttc gccggccggt gtgggcgt
//
LOCUS       DATLGF010000396         3285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_140905, whole genome shotgun sequence.
ACCESSION   DATLGF010000396 DATLGF010000000
VERSION     DATLGF010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140905"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..786
                     /locus_tag="VM406_12155"
     CDS             205..786
                     /locus_tag="VM406_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HVL76760.1"
                     /translation="MKAITTLSDGIARAERGAVRVIVLALPIMILINATARALRTPVY
                     WMDELAILTMVWLAMIGMSLTLKSRDAVSVTMLVDAVPPLLMKGMKILIDVAVLTFGI
                     ALLVLCYRWFDPITLMRLGFDTAEFSGETFNFMYQDTTATLGVQKFWFWLVVPLVAFT
                     TSVHALSNLLHTLAAPARAQVDEVPAASAAAAE"
     gene            791..2056
                     /locus_tag="VM406_12160"
     CDS             791..2056
                     /locus_tag="VM406_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HVL76761.1"
                     /translation="MIAVIFTSLLFLGLPIALVLAMTALAYIYLSGNTVLYLSFPQQF
                     FAGLENYGLLAIPLFMLVGELMNEGGITRRLVALASVFVGHVRGGLAYINILANMFLA
                     SIVGSANAQVAVMAQVMVPEMEKKGYDPGFATATTCAGALLAPIIPPSMLFVIFGVLA
                     QISIGDLFIAGIIPGLLMAFAFICVVALLGWRYKYPPGERLNRKQKVHNIGAAIPSML
                     VPAVMIGGILGGLTTPTEAAALGALMAVLVGIFAHKEIRSSRIGGMLLRVALNSGVVL
                     VLVAAASVFGWVIVFEKIPQQLAVLLQQITSDPFMFMLLVTVLLLGVGTVIDGIAALI
                     LLVPILLPVATQVYGINPYHFGVVVCVNLTLGLLTPPVGAALYVAARVTGVKPAQIIR
                     PLLPFLGVTLLILVLLSWQPQLVTMLLNR"
     gene            2111..2869
                     /locus_tag="VM406_12165"
     CDS             2111..2869
                     /locus_tag="VM406_12165"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HVL76762.1"
                     /translation="MTKLKDRVAIVTGGAQGIGAAYARALAAEGARVAVADILDGTAV
                     AKDIEAAGGQAMSVTVNVADEASANAMAQAVIEHFGRIDILVNNAAVYASLQLKPFTE
                     IDVAEWDKVMAVNVRGPFLCAKAVVPFMRREGYGRIVNISSGTPFKGTPNLLHYVTSK
                     GAILAFTRALAREVGGDGICVNTLAPGLVLSEGVIQNEAMREKLTGPVIASRAIKRDQ
                     MPEDLIRPLLFLASDDCAFMTGETVVVDGGSVMH"
     gene            2893..>3285
                     /locus_tag="VM406_12170"
     CDS             2893..>3285
                     /locus_tag="VM406_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVL76763.1"
                     /translation="MTSIAQPWIGSQWAPFGGAAADSIDPSTGEVLGQFADADVGVAE
                     AAIAAARQAFIYSDWAHAPRLRAAVMLEFADRVERRSEELAMLLARETGKLLKPAQAE
                     LAGTISEIRFYAGLARTIFGRTTEVEPGA"
BASE COUNT          518 a         1068 c         1087 g          612 t
ORIGIN      
        1 ggtcaaggtg gtgccggccg ggccggaatt cttcggcgac atgctcaagc agtgggaaga
       61 gatctggctc aagcgcaccc cggtgctggc cgatctgcgc cgcgaggccg ccgccatcaa
      121 gtaaatccga ggccgtgccc aggcgctccc cgcccgtggc ggtgagcgcc ggagcggaac
      181 cgacaagccg gagagtttct cgtggtgaaa gccattacta ccctgagcga cggcatcgcg
      241 cgcgccgagc gcggcgccgt ccgcgtcatc gtgctcgcgc ttccgatcat gatcctgatc
      301 aacgcgacgg cccgggcctt gcgcacgccg gtctactgga tggacgagct ggcgattctg
      361 acgatggtct ggctggcgat gatcggcatg tcgctcacgc tgaagtcgcg cgacgcggtg
      421 tcggtcacga tgctggtcga cgccgtcccc ccgctgctga tgaaggggat gaagatcctg
      481 atcgacgtgg cggtcctgac cttcggcatc gcactgctgg tgctctgcta tcgctggttc
      541 gaccccatca ccctgatgcg tctgggtttc gataccgccg agttctcggg cgagaccttc
      601 aatttcatgt accaggacac gaccgccaca ctgggcgtgc agaaattctg gttctggctc
      661 gtggtgcccc tggtcgcgtt caccaccagc gtgcacgcgc tttccaacct gctgcatacg
      721 ctggctgcgc cggcacgggc gcaggtggac gaagtccccg cggccagtgc cgccgcagcg
      781 gagtaagccc atgattgccg ttatctttac ctcgctgctg ttcctcgggc tgccgatcgc
      841 gctggtgctc gcgatgaccg cgctggccta catttatctg agcggcaaca cggtcctcta
      901 cctttcattt ccgcagcagt tcttcgccgg actggagaac tacgggctgc ttgcgatccc
      961 gctgttcatg ctggtcggcg aactgatgaa cgaagggggc atcacccgcc gcctggtcgc
     1021 gctggcctcg gtgttcgtcg gccatgtgcg cggcgggctc gcctacatca acatcctggc
     1081 caacatgttc ctggcctcga tcgtcggctc ggcaaatgcg caggtcgccg tgatggccca
     1141 ggtgatggtg ccggagatgg aaaagaaggg ttacgacccc ggttttgcga ccgccactac
     1201 ctgcgcgggc gcgctgctgg cgcccatcat cccgccgtcc atgctgttcg tgatcttcgg
     1261 cgtgctggcc cagatttcga tcggcgacct gttcatcgcc ggcatcattc ctgggctctt
     1321 gatggcgttc gcgttcatct gcgtcgttgc cttgctcggc tggcgctata aatatccgcc
     1381 cggcgaacgg ctgaaccgta agcagaaggt gcacaacatc ggcgcggcga ttccgtcgat
     1441 gctggtgccg gcggtgatga tcggcggcat tctcggcggc ctgacgacgc cgacggaagc
     1501 ggcggcgctt ggcgcgctga tggcggtgct ggtcgggata ttcgcgcaca aggaaatccg
     1561 ctcgtcgcgc atcggcggca tgctgctgcg cgtggcgctc aacagcggcg tcgtgctggt
     1621 cctggtggcg gcggccagcg tgttcggctg ggtcatcgtg ttcgagaaga tcccgcagca
     1681 gcttgcggtg ctgctgcagc agatcacctc ggacccgttc atgttcatgc tgctggtcac
     1741 cgtgctgctg cttggtgtgg gcaccgtgat cgacggcatc gctgcgctga tcctgctggt
     1801 gcccatcctg ctgccggtcg cgacccaggt gtacggcatc aacccgtatc acttcggcgt
     1861 ggtggtctgc gtgaacctga cactgggcct gctgacgccg cctgtcggtg cggcactgta
     1921 tgtcgccgcg cgcgtgacgg gcgtgaagcc ggcacagatc atccggcccc tgctgccgtt
     1981 cctgggcgtc accctgctga tcctggtgct gctgtcctgg cagccgcaac tcgtaaccat
     2041 gctgctcaac aggtaatcgg gcagggcgcc gcactgcagc ggcgcccgct tcatctttac
     2101 ggaggaatca atgacgaagt tgaaagatcg cgttgccatc gtcaccggtg gcgcgcaagg
     2161 gataggcgcg gcctacgcgc gcgcactggc ggccgaaggc gcgcgggtcg ccgtcgccga
     2221 tattcttgac ggcaccgccg tcgctaagga catcgaggcc gccggcgggc aggcgatgtc
     2281 ggtcacggtc aacgtggccg acgaagcctc cgccaatgcg atggcgcagg ccgtgatcga
     2341 acacttcggg cggatcgaca tcctggtcaa caacgccgcg gtctacgcat cgctgcagct
     2401 caagccgttc actgaaatcg acgtggcgga gtgggacaag gtgatggcgg tgaatgtgcg
     2461 cgggcccttc ctgtgcgcga aggcggtggt gccgttcatg cgccgcgaag gctacggccg
     2521 catcgtcaac atctcgtccg gcacgccgtt caagggtacg ccgaacctgt tgcactacgt
     2581 gacctccaag ggcgcgatcc tggcgttcac gcgcgcgctg gcgcgcgagg tcggcggtga
     2641 cggcatttgc gtcaatacac ttgcgccggg actcgtactc agcgaaggcg tgatccagaa
     2701 cgaggcgatg cgcgaaaaac tgaccgggcc cgtgatcgcc agccgagcga tcaagcgcga
     2761 ccagatgccg gaagacctga tccggccgct gctgttcctt gcctccgacg actgcgcctt
     2821 catgacaggc gagacggtcg tcgtcgacgg cggctccgtc atgcattgaa ccagaaccgg
     2881 aaaggaaacc agatgacttc cattgcccaa ccgtggatcg gcagtcagtg ggcgccgttc
     2941 ggcggcgccg ctgccgacag catcgacccg agcaccggcg aagtgctcgg ccagtttgcc
     3001 gacgccgacg tcggcgtggc cgaagcggcc atcgcggcgg cgcgccaggc cttcatctac
     3061 agcgactggg cgcacgcgcc gcgcttgcgc gcggccgtga tgctcgagtt cgccgaccgc
     3121 gtcgagcgcc gcagcgaaga gctcgcgatg ctgctcgcac gcgagaccgg caagctgctc
     3181 aagcccgccc aggccgagct cgccggcacg atctccgaga tccgcttcta cgccggcctt
     3241 gcgcgcacga ttttcggacg caccacggag gtggagccag gtgcc
//
LOCUS       DATLGF010000397         5596 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_183220, whole genome shotgun sequence.
ACCESSION   DATLGF010000397 DATLGF010000000
VERSION     DATLGF010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183220"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VM406_12175"
     CDS             complement(<1..823)
                     /locus_tag="VM406_12175"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVL76764.1"
                     /translation="MTAASAIASGYPTKPITMIVPFAAGGSTDGVARAIAQQLGERLG
                     KPVIVENKAGAGGTIGSHFVARAQPDGYTILLGTVSTNAVAGSLYERLPYDTVKSFTP
                     ITEIATVPELLVVHPSVPAKNVKELVEYVKARPNQLSFASAGPGSASHLAAELFMSMT
                     GTKMVHVPYRGSGPALQDTLGNHTQVMFDVVMTSHSHVRAGTLRALGVTSSARSSVVP
                     DVPTVAEAGVPGYEAIVWFGVFGPAGMPADVTNRLQQEIAAALKTPKLQELLAAQG"
     gene            complement(928..2676)
                     /locus_tag="VM406_12180"
     CDS             complement(928..2676)
                     /locus_tag="VM406_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HVL76765.1"
                     /translation="MSDIQHSERKFDPIGMEIFSNRLQTITEEMGNNLIRSAFSTNIK
                     ERRDCSVGLFDARGRLVAQASHIPLHLGSLKGGVEAVLAAVDIARMLPGDAYMCNDPY
                     LAGGTHMPDISIVTPVFHDGQVRFFTANIAHHSDIGGSVPGSISGGARSVFEEGLRIP
                     VIRIVRGGELDADLLNLLANNTREPEERMLDLKVQIATNQRGAVAVEQLIAQMGLANV
                     ENMIEDVLDYTARRLRNRVRELKSGSYAFTSHLDEDGSGTGDPVPIAVTVTVDGERLK
                     LDFTGSGVQARGALNVPRSALEATVYYAVKALLDPQLMPNHGMFAAIEIFAPEGTITN
                     PRFPAAVGARSITCQKIAGAIFGAFRGVLPKEKVLASGNDLLPGIVFSGRQKRRDGTY
                     VYMETVGGGSGAGFVRDGMDAVQVHVTNSSNLPVEALENEYPLRIVEYALVQDSGGAG
                     RQRGGLGIAREVMALEDGTVFTARSDSHLFGPAGAFGGKDGGRARLIRNPGTPNEAAL
                     PSKTSNLVLKAGETVRIETPGGAGYGPPEDRSPALLAADLRGGKVSRSAAQRDYGPDK
                     LAMALGSGTEATAANK"
     gene            complement(2673..4739)
                     /locus_tag="VM406_12185"
     CDS             complement(2673..4739)
                     /locus_tag="VM406_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HVL76766.1"
                     /translation="MYRIGIDVGGTFTDFTLLNEDTFEIAYFKISSTPKDPSEAIRNG
                     IAQMLTEHRIAPEQLSFVGHGTTVATNMIIERRGVPTGLITTRGFRDVLEIGRQTRPN
                     LFDYSDGKPEPLVAREHRFEITERMGADGEVITPLDRNELEAAIAQLREAGVQAVAIC
                     FLHAYRNPAHEQEAREMVERMLPNVYVSVSSEVLPEFREYERLSTTVLNAYSGPKMQA
                     YLDRLSNGVREMGIPQDPYTIHSNGGLMSIPTVRRFPVRTCLSGPASGVVGSAVVGMA
                     AGFPNVITFDVGGTSTDVSVIKDGKPVFTSNRLVADYPIKMPMVDIHVIGAGGGSVAW
                     IDDAGALKAGPHSAGALPGPVAYGRGGTEPTTTDVNVCLHRLNPVSLLRGRLAIDEAG
                     ARNVIDTKVAQPLGLGLEAAAHGILRIVTANMSRAIRSVSTERGYNLSEFALYAYGGA
                     GPLHAAEVAEECGIPTVIVPQEPGTMCARGILLSDVSLDFVRTEIAPANEETWAKVCA
                     FFDDMTRDGTAWLEQERVVPAQRAFRRYVEARYEGQNYEIAVELDSVDPNGLQAYLEQ
                     FRAAHVKEYGYAIPGRTVQIVNCRTEAIGRVPKAELRKAGEGRAANVADAAIGVREVY
                     FGEGAGWVPTTVYERSLLPVDTAIAGPAIIEEMSSTTVVLPRHSFKVDTLGNIIIRVR
                     PEEHAA"
     gene            4921..>5596
                     /locus_tag="VM406_12190"
     CDS             4921..>5596
                     /locus_tag="VM406_12190"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL76767.1"
                     /translation="MEIYQLRAFVAVARHGNFTKAASELHLSQPTVSGQIKALEEDLG
                     VLLFERRASGVVTTATGAALLPKAEKLLADVRGFKAHARQMGAQLPAKILLGTVIDAR
                     FLRLGQFMSAMRARHPQVEIETHHGLSGWVMNAVRKGDLDCGFFVGPVRQADVTATLL
                     TEMTYRIVAPCDWAVRIADAGWAEIAAMPWIWAPEEGSYPQIAMEMFREHGVEPNKVA
                     VADRDLV"
BASE COUNT          866 a         1816 c         1907 g         1007 t
ORIGIN      
        1 caccctgggc ggccagcagc tcctgcagct tcggggtctt cagcgcggcg gcaatctctt
       61 gctgcaggcg gttggtgaca tcggcgggca tgccggccgg gccgaacacg ccgaaccaga
      121 cgatcgcctc atagccgggc acgccggctt cggccaccgt gggcacgtcc ggcacgacgg
      181 aggaacgcgc gctgctggtg acgccaagcg cgcgcagcgt gccggcacgc acgtgcgagt
      241 gcgaggtcat caccacgtcg aacatcacct gggtgtggtt gcccagcgtg tcctgcagcg
      301 ccgggccgct gccgcggtac ggcacgtgga ccatcttggt gccggtcatc gacatgaaca
      361 gttcggcggc caagtggctt gcgctgcccg ggccggccga cgcaaacgag agctggttcg
      421 ggcgtgcctt cacgtactcg accagttcct tcacgttctt cgcgggcacc gacggatgga
      481 cgaccagcag ttcgggaacg gtcgcgatct cggtgatcgg cgtgaagctc ttcacggtgt
      541 cgtacggcag ccgctcgtac agcgagccgg ccacagcatt ggtgctgaca gtgccgagca
      601 ggatcgtgta gccgtcgggc tgcgcgcgcg cgacgaagtg cgaaccgatg gtgccgccgg
      661 cgccggcctt gttttcgacg atgaccggct tgccgaggcg ttcaccgagc tgctgggcga
      721 tcgcgcgggc gacgccgtcg gtggaaccgc cggcggcgaa cggcacgatc atcgtgatcg
      781 gtttggtcgg atagccactg gcgatggcgc tggccgcagt catgctcaga acgcatgcgg
      841 aaagaacgcg ggacagaagt ttcattgaat cactccctga aaatgtaaaa aggcgagaac
      901 cgggtcaccg ggcctcgccg ctctgtgcta cttgtttgct gctgttgcct cggtcccgct
      961 gcccagcgcc attgccagct tgtcggggcc gtagtcgcgt tgtgcagcac tacgcgatac
     1021 cttgccgccg cgcaggtctg ctgcgagcag cgccggcgag cggtcttccg gcgggccgta
     1081 tccggccccg cccggtgttt caatcctgac cgtctcccct gccttcagca ccaggttcga
     1141 ggtcttcgac ggcagtgccg cctcgttcgg ggtgcctgga ttgcggatca ggcgcgcccg
     1201 tccgccatcc ttgccgccga aggcgccggc cggaccgaac aggtggctgt cggagcgggc
     1261 cgtgaagacc gtgccgtcct ccagcgccat cacttcccgc gcgatgccca ggccgccgcg
     1321 ctgccgccct gctccgcccg agtcctgcac cagcgcatac tcgacgatgc gcagcgggta
     1381 ttcgttttcc agcgcttcga ccggcaggtt cgagctgttc gtcacgtgca cctgcaccgc
     1441 gtccatgccg tcccggacga agccggcgcc ggagccgccg cccaccgtct ccatgtacac
     1501 ataggtgccg tcacggcgct tctggcggcc ggaaaacacg atgccgggca gcaggtcgtt
     1561 gccggaggcg agcaccttct ccttcggcag cacgccgcgg aaggcgccga agatcgcgcc
     1621 ggcgatcttc tggcaggtga tgctgcgcgc gccgaccgca gccgggaagc gcggattggt
     1681 gatcgtgcct tcgggcgcga agatttcgat cgccgcgaac atgccatggt tgggcatcag
     1741 ctgcggatcg agcagcgcct tgactgcgta gtagacggtc gcttccagtg cgctgcgcgg
     1801 cacgttcagc gcgcccctgg cctggacgcc ggaaccggtg aaatcgagct tcaggcgctc
     1861 gccgtcgacg gtgacggtga ccgcgatcgg caccgggtcg ccggtgcccg agccgtcctc
     1921 gtccagatgg ctggtgaacg catagctgcc gctcttcagt tcgcgcaccc ggttgcgcag
     1981 gcggcgcgcg gtgtagtcga gcacgtcctc gatcatgttc tcgacgttcg ccagccccat
     2041 ctgggcgatc agctgttcca ccgcgacggc gccgcgctgg ttggtggcga tctgcacctt
     2101 caggtcgagc atgcgctctt ccggctcgcg ggtgttgttg gcaagcaggt tcagcaggtc
     2161 ggcgtcgagt tcgccgccgc gcacgatgcg gatcaccggg atgcgcaggc cttcctcgaa
     2221 cacgctgcgc gcaccgcccg agatcgaacc gggcacgctg ccgccgatat cggagtggtg
     2281 cgcgatgttg gcggtgaaga agcgcacctg cccgtcatgg aacaccggcg tgacgataga
     2341 gatgtccggc atgtgcgtgc cgccggccag gtacgggtcg ttgcacatgt aggcgtcgcc
     2401 gggcagcatg cgcgcgatgt ccaccgcggc aagcaccgct tccacgccgc ccttgagcga
     2461 accgaggtgc agcggaatat gcgaagcctg ggcgaccagc cggccgcgcg cgtcgaacag
     2521 gccgaccgag cagtcgcgcc gctccttgat gttggtggaa aaggcggagc ggatcaggtt
     2581 gttgcccatc tcctcggtga tggtctgcag ccggttgctg aagatttcca tcccgatcgg
     2641 gtcgaacttg cgttcgctgt gttgaatgtc gctcatgctg cgtgttcctc ggggcgtacc
     2701 cggataatga tgttgcctag cgtgtcgacc ttgaagctgt gccgcggcag cacgaccgtg
     2761 gtcgaactca tttcctcgat gatggccgga ccggcgatcg cggtgtccac cggcagcagg
     2821 ctgcgctcgt agacggtggt cggcacccag ccggcgcctt cgccgaagta gacttcgcgc
     2881 acgccgatcg cggcatcggc cacgttcgcc gcgcggcctt cgccggcctt gcgcagctcg
     2941 gccttcggca cgcggccgat cgcctcggtg cggcagttca cgatctgcac ggtacgtccc
     3001 gggatggcgt agccatattc cttcacatgc gcggcacgga actgctcgag ataggcttgc
     3061 aagccgttcg gatcgacgct gtcgagttca acagcgatct cgtagttctg gccttcatag
     3121 cgcgcttcca catagcgccg gaacgcgcgc tgtgcgggca ccaccctctc ctgttcgagc
     3181 caggccgtgc catcgcgtgt catgtcgtcg aagaacgcgc agaccttggc ccaggtttcc
     3241 tcgttggccg gcgcgatctc ggtgcgcacg aaatccaggc tcacgtcgga gagcaggatg
     3301 ccgcgagcgc acatcgtgcc cggctcctgc gggacgatca cggtcgggat gccgcattcc
     3361 tcggccactt ccgccgcgtg cagcggaccg gcgccgccgt acgcatacag tgcgaattcg
     3421 gacaggttgt agccgcgttc ggtcgagacc gagcggatcg cccggctcat gttcgcggtg
     3481 acgatgcgca ggatgccgtg ggcggcggct tcgaggccga ggccgagcgg ctgcgcgacc
     3541 ttggtgtcga tcacgttgcg cgcgccggcc tcgtcgatcg ccaggcgacc gcgcagcagc
     3601 gacaccgggt tcagccggtg caggcagacg ttgacgtcgg tggtcgtcgg ttcggtgccg
     3661 ccgcggccgt aggcgaccgg ccccggcaag gcgcccgcgc tgtgcggtcc ggccttcagc
     3721 gctccggcgt cgtcgatcca ggccacgctg ccgccgccgg cgccgatcac gtggatgtcg
     3781 accatcggca tcttgatcgg gtagtcggcc accaggcggt tcgaggtgaa caccggcttg
     3841 ccatccttga tgaccgagac gtcggtgctg gtgccgccga catcgaaggt gatcacgttc
     3901 ggaaagcctg cggccatgcc gaccaccgcc gagccgacca cgccggaggc gggaccggac
     3961 aggcaggtgc gtaccgggaa gcggcgcacg gtgggaatcg acatcaggcc gccgttggaa
     4021 tggatcgtgt acggatcctg cgggatgccc atttcgcgca cgccattgga caggcgatcc
     4081 aggtaggctt gcatcttcgg gcccgagtag gcgttgagca ccgtggtcga caggcgctcg
     4141 tactcgcgga actcgggcag cacctcggac gacacgctca catacacatt tggcagcatg
     4201 cgctcgacca tctcgcgcgc ctcttgctcg tgcgccggat tgcggtaggc atgcaggaag
     4261 cagatcgcga ccgcctgcac gccggcttcg cgcagttgcg cgatcgcggc ttcgagttca
     4321 ttcctgtcca gcggcgtgat gacttcgccg tcggcgccca tgcgctcggt gatctcgaag
     4381 cgatgctcgc gggcgaccag cggttccggc ttgccgtcgc tgtagtcgaa caggttcggg
     4441 cgggtctggc ggccgatctc cagcacgtcg cggaaaccgc gcgtcgtgat caggccggtc
     4501 ggcacgccgc ggcgctcgat gatcatgttg gtggccaccg tcgtgccgtg gccgacgaag
     4561 gaaagctgtt ccggcgcgat gcggtgctcg gtcagcatct gggcgatgcc gttgcggatc
     4621 gcctcggacg gatctttcgg agtcgacgaa atcttgaagt aggcgatctc gaaggtgtcc
     4681 tcgttgagca gcgtaaagtc ggtgaaggtg ccgccgacgt caatgccaat tctgtacatg
     4741 cgtgtggttc ctgtttgcgt gccggtcagc ggcgcgaccg gtgccgcgtt gtgccgtgtt
     4801 tccgttgcat gacactctag ggaggcgcgg tcacagcgtc caacggtcaa tttcgatgga
     4861 gctatcggaa attccgataa cgcattaacc ttcgggtttt cccccgcatg ccctgcaccc
     4921 atggagatct atcaactgcg cgccttcgtt gctgttgcca ggcatggcaa tttcaccaag
     4981 gcggccagcg agctgcacct gagccagccg accgtcagcg gccagatcaa ggcgctggaa
     5041 gaggatctcg gcgtgctgct gttcgaacgg cgcgcgtcgg gtgtcgtgac cacggcgacc
     5101 ggcgcggcgc tgttgccgaa agcggaaaag ctccttgccg acgtgcgcgg cttcaaggcg
     5161 cacgcgcggc agatgggcgc gcaactgccc gcgaagattc tcttaggcac tgttatcgac
     5221 gcaaggtttc ttcgcctggg gcaattcatg agcgcgatgc gcgcccgtca tccgcaggtc
     5281 gagatcgaaa cccaccacgg cttgtccggc tgggtgatga acgcggtgcg caagggcgat
     5341 ctggactgcg gtttcttcgt cggcccggtg cggcaagccg acgtgaccgc gacgctgttg
     5401 accgaaatga cataccggat cgtcgcgccc tgcgactggg ccgtgcgcat tgccgacgcg
     5461 ggctgggccg agatcgcggc aatgccgtgg atctgggcgc cggaggaagg ctcgtatccg
     5521 cagattgcga tggaaatgtt tcgtgaacac ggcgtcgagc cgaacaaggt cgcggtcgcc
     5581 gaccgcgacc ttgttc
//
LOCUS       DATLGF010000398         2911 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_172335, whole genome shotgun sequence.
ACCESSION   DATLGF010000398 DATLGF010000000
VERSION     DATLGF010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172335"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VM406_12195"
     CDS             <1..437
                     /locus_tag="VM406_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HVL76768.1"
                     /translation="RMLQSSELLLAQEFLPTEFDWRIGVLDGRALFVCKYYMAPGHWQ
                     VIKHESQRMSEGRTEALSIGEAPDEVVRTAVEAAALIGDGFYGVDLKQVGSQVVVIEI
                     NDNPNVDAGNEDAVLGDALYREVMGVFRKRIGADAAMRTRAP"
     gene            434..1666
                     /locus_tag="VM406_12200"
     CDS             434..1666
                     /locus_tag="VM406_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HVL76769.1"
                     /translation="MTVSAFAAFQCVGIEIEYMIVADDDLRVLPIADRILQHEGVPCA
                     EVARGALGWSNELVLHVLEVKNREPAASLAELAPAFAAEPGELERRLAPFGARLMPGA
                     MHPWMNPREETRLWPHDNDAIYRAYHRIFDCCAHGWANLQSMHVNLPFRGDEEFARLH
                     AAVRCVLPILPALAASSPFAEGRATGYADYRMQVYWNNAAAFPAITGEVIPENASSHA
                     EYAMRILEPMYRAIVPLDPEQVLQHEWLNSRGAIARFDRSAIEIRVIDSQECPQADLA
                     IAAAAIGAARMLYETGDLAAQQNLSTQALAKLLRACVRDGENAGIDNDDYLRLFGLGT
                     GPRAAAKVWDALIDRLAAREPDWQQWARPLSTILAQGTLSTRLLRAAGASPSRERLAG
                     IYRTLCDCLREGRQFMAD"
     gene            1667..2371
                     /locus_tag="VM406_12205"
     CDS             1667..2371
                     /locus_tag="VM406_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HVL76770.1"
                     /translation="MPARFCLISCEHAGNRIPSRYADFFRGHGALLRSHRGYDIGSLR
                     MARELARALDAPLVISPVSRLLIDLNRSIGQPDLYSELTRDIDPALREEIVNRYYLPF
                     RGKVMTRVAQALDGGASVLHVSSHSFTPEWQGMSREADVGLLFDPGRGPEVSLCERWG
                     AALRRIAPGLTVRMNYPYLGTADGLTTSLRGLHGERYLGIELEINQKHVRAGRHAWAA
                     VRTAVCRALLEAMSTA"
     gene            2453..2755
                     /locus_tag="VM406_12210"
     CDS             2453..2755
                     /locus_tag="VM406_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76771.1"
                     /translation="MTEVFTREPETVIKTLQDAGLCRAQAPARETADCPAAQSCLLAD
                     GAQLCVQGVQTAGTAVPAAPAANEAREITGMEVVSVAAVFIAGIALGRYWPRSRSR"
BASE COUNT          478 a          987 c          992 g          454 t
ORIGIN      
        1 atcgcatgct gcagagctca gaactgctgc tggcccagga attcctgccg accgaattcg
       61 actggcgcat cggtgtgctc gacggacgtg cgctgttcgt ctgcaagtac tacatggcgc
      121 ccggccactg gcaggtcatc aagcacgagt cgcagcgcat gagcgaaggc cgcaccgaag
      181 cgctctcgat cggcgaagcg ccggacgaag tggtgcgcac cgcggtcgag gcggcggcgc
      241 tgatcggcga cggcttctac ggcgtggatc tgaagcaggt cggcagccag gtggtggtca
      301 tcgagatcaa cgacaacccg aacgtcgacg ccggcaacga ggacgcggtg ctgggggatg
      361 cgctctatcg cgaggtgatg ggcgtgttcc gcaagcgcat cggtgccgat gccgcaatga
      421 ggacgcgcgc accatgaccg tttcggcatt cgcggccttc cagtgcgtgg gcatcgagat
      481 cgagtacatg atcgtcgcag acgatgacct gcgtgtgctg ccgatcgccg accgcatcct
      541 gcagcatgag ggcgtcccgt gcgcggaggt cgcgcgcggg gcgctcggct ggtcgaacga
      601 actggtgctg cacgtgctgg aggtgaagaa tcgcgaaccg gcggcaagcc ttgccgaact
      661 tgcgccggca tttgccgccg agcccggcga actcgagcgg cggctggcgc cattcggcgc
      721 gcgcctgatg ccgggcgcga tgcatccgtg gatgaatccg cgcgaggaaa cccggctctg
      781 gccgcacgac aacgacgcga tctaccgcgc ctatcaccgc attttcgatt gctgtgcgca
      841 cggctgggcg aatctgcaga gcatgcacgt gaatctgccg tttcgcggcg acgaagagtt
      901 cgcccgcctg catgccgccg tgcgctgcgt gctgcctatc ctgccggcgc ttgcggccag
      961 ttcgccgttc gcggaggggc gcgccaccgg ctatgcggac taccggatgc aggtgtactg
     1021 gaacaatgcc gcagccttcc cggccatcac cggagaggtc ataccggaga acgccagctc
     1081 ccacgcggag tatgcgatgc gcatcctcga accgatgtac cgggcgatcg tcccgctcga
     1141 ccccgagcag gtgctgcagc acgaatggct caattcacgc ggggcgatcg cccgcttcga
     1201 ccgcagcgcg atcgagatcc gcgtgatcga cagccaggaa tgcccgcagg ccgatctcgc
     1261 gatcgctgcg gcggcgatcg gcgcggcgcg catgctttac gagacgggcg acttggccgc
     1321 acagcagaac ctgtccacgc aggccctggc gaaactgctg cgcgcctgcg tgcgcgacgg
     1381 cgagaacgcc ggcatcgaca acgacgacta cctgcgcctg ttcggactcg gcaccggccc
     1441 gcgtgccgcg gcgaaagtgt gggacgcact gatcgaccgg ctggccgcgc gcgagcccga
     1501 ctggcagcaa tgggcccgcc cgctgtccac aatcctcgcg cagggcacgc tgtccacccg
     1561 cttgctgcgc gccgccggcg cgtccccgtc gcgcgagcgg ctcgccggaa tctatcgcac
     1621 cctgtgcgac tgcctgcgcg aaggccggca gttcatggcg gattagatgc cggcccgctt
     1681 ctgcctgatc agttgcgagc atgccggcaa ccgcattcct tcgcgctatg ccgatttctt
     1741 tcgcggccat ggcgcgctgc tgcgcagcca tcgcggctac gacatcggct cgctgcgcat
     1801 ggcacgcgaa ctggcgcgtg cgctcgatgc gccgctggtg atctccccgg tcagccggct
     1861 gctgatcgac ctgaaccgtt cgatcggcca gcccgatctg tattcggagc tcacgcgcga
     1921 catcgatccg gcgctgcgcg aggagatcgt caaccgctac tacctgccgt ttcgcggcaa
     1981 ggtcatgacc cgcgtcgccc aggcgctcga cggcggcgcc agcgtgctgc acgtctccag
     2041 ccacagcttc acgccggagt ggcaaggcat gtcgcgcgag gccgacgtgg gcctgctgtt
     2101 cgatcccggg cgcggcccgg aagtgtcgct gtgcgagcgc tggggcgcgg cgctgcggcg
     2161 gatcgcgcct gggctgacgg tacgcatgaa ctacccctac ctcggcactg cggacggcct
     2221 gacgaccagc ctgcgcggcc tgcatggcga gcgctatctc ggcatcgagc tggagatcaa
     2281 ccagaagcac gtgcgcgccg gccgccacgc gtgggcggcg gtgcgcacgg cggtgtgccg
     2341 cgcgctgctg gaagcgatgt cgacagcatg atcagtgcaa gacgcaatct ggcggcgtgc
     2401 ggcatcacca cgcgaagtat catgacgcgc ctactgccag atggagcccg ccatgaccga
     2461 agtgttcacc cgcgaaccgg aaaccgtgat caagaccttg caggatgccg gcctgtgccg
     2521 cgcgcaggcg ccggcacgcg agacggcgga ttgcccggcc gcgcagtcct gcttgctggc
     2581 agacggtgcg cagctgtgcg tgcagggcgt gcagaccgcg ggcacggccg ttcccgcggc
     2641 gccggcggca aacgaagcgc gcgagatcac gggaatggaa gtggtatcgg tggccgccgt
     2701 gtttatcgcc ggcattgccc ttggccgcta ctggccgcgc tcgcgcagca ggtagccggg
     2761 catgcgtttc gctagcgcca gcaaggatgc acgccctcct gccttgctcc gaccacgcgc
     2821 gcccatgccc gacaagctcg agaatctcca gcacatgctt gaccagatcg aacaggccga
     2881 aaccgccgag gaccgcgtat cgctgcacgc g
//
LOCUS       DATLGF010000399         2921 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_103852, whole genome shotgun sequence.
ACCESSION   DATLGF010000399 DATLGF010000000
VERSION     DATLGF010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103852"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VM406_12215"
     CDS             complement(<1..536)
                     /locus_tag="VM406_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HVL76772.1"
                     /translation="MDIRPEWTDELDVKVLLEVLTAVKKGDFSARMPAHWSGPAGKIA
                     DTLNDIIETNDKLAGEVEEVARAVGREGRLNQRASSAGTGGGWSDIVKSVNTLIDDLV
                     HPTTELARVVSGVAKGDLSQRVGTEVDGRPLMGQFLTAATAANTMVDQLSAFTSEAAR
                     LAHEVGNEGRLGGAAHVPG"
     gene            complement(809..2083)
                     /locus_tag="VM406_12220"
     CDS             complement(809..2083)
                     /locus_tag="VM406_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020693841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL76773.1"
                     /translation="MFTPKVLLVNDDAASLLALASLLSQDQGHEVITARSGEEALRHV
                     LKHDFAVILLDVSMPGMDGFETAEAIHSHPRSASVPIIFVTAHYSDEMHRLKAYQRGA
                     VDYLFTPIIPQILQTKVAVFVELAKKNLQLQHQKQELEQLNQALQEQRRQDLEHINER
                     RQAEEALRQSREELRQLASYQERIKEDERKRIAREIHDELGQNLLALRIDVAMLHART
                     GAAHPKLNKKVNAVLDHIDSTMKAMRAIINNLRPTVLDLGLSAAIEWQVKEFQRRTGI
                     VCELTMADKDMVVDDNRATALFRILQESLNNVFRHARATRARIEVQKRDGHLFMSVAD
                     NGVGIFPGCRRKANSFGLVGIKERVSTLGGDFQIESGEEKGTSLTVSIPVAPESDAAA
                     AAPPSAAAATSTARGQRRSGRRKNEAGTEKVN"
     gene            complement(2275..>2921)
                     /locus_tag="VM406_12225"
     CDS             complement(2275..>2921)
                     /locus_tag="VM406_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HVL76774.1"
                     /translation="VIEITADDIDRLKNSSSRLRVGTRMTRAEALHLSLMSSENRAAS
                     ALARHYPGGTPAFVAAMNARARSLGMRNTRFVESTGLSSENVASAHDLTRLAVAAYRH
                     PLIRDYSTHPRHAIAAAGGHQLQYMNSNRLVDHPDWDIGLQKTGYISEAGRCLLMQAR
                     VDGRPVVMVFLDSRGKDSRLGDAARVRKWLETAEQTTMTTASARSSCFSAPPGA"
BASE COUNT          497 a          925 c          943 g          556 t
ORIGIN      
        1 ccggggacat gcgcagcgcc acccaggcgc ccctcgttcc cgacttcgtg cgcaagacgc
       61 gcggcctccg acgtgaatgc cgacagctgg tcgaccatcg tgttggccgc cgtggcagca
      121 gtgaggaact ggcccatcag cggccggccg tcgacctcgg tgccgacgcg ctgggacaga
      181 tcgcccttgg caacgccgct gaccacgcgc gccaattctg tggtcggatg cacgagatcg
      241 tcgatcaggg tattgaccga cttgacgatg tcggaccacc cgccgccggt cccggcactg
      301 gaggcgcgct ggttcaggcg cccctcgcgc cccaccgcac gggcaacctc ctcgacctca
      361 cccgccagct tgtcattggt ttcgatgatg tcgttgagcg tgtcggcgat cttgcccgcc
      421 ggcccgctcc aatgtgccgg catgcgcgcc gagaagtcgc ccttcttcac cgccgtcagc
      481 acttccagca ataccttgac gtcgagttcg tcggtccatt ccggccttat atccacacca
      541 gttctcctga tcgttcctgc aaagggtcgc cccgccttcc ggccttgagt ccggagggcg
      601 gggctgggat gcatgagttc agccggtcaa acgccggcaa gcgatgctgc agccccggct
      661 tgtcggggcc ttcgcgcgac gcgtgttgtc gtcgggcaaa tcgagcggct attgtctggc
      721 aaagggcgta cagcggcaac ggagcaatgc tcgggggcgc ggctcaaatc aagcagtatt
      781 gaggattctc aaccctgatg cccgattgtc aattgacctt ttcggtgccg gcttcgttct
      841 tgcgccggcc gctgcggcgc tggccgcgcg cggtggaggt ggcggcagca gcggaaggcg
      901 gcgcagcggc agcggcatcg gactcgggtg cgaccggtat ggatacggtc agcgaggtgc
      961 ccttctcttc tcccgactcg atttggaagt cgccgcccag cgtgctcaca cgctccttga
     1021 tgccaaccag gccgaaggaa ttggccttgc gacggcagcc agggaagatg ccgactccgt
     1081 tgtcggcgac gctcatgaac aggtgcccgt cgcgcttctg aacctcgatg cgcgcgcggg
     1141 tggcgcgtgc gtgacggaac acattattga gtgactcctg cagaatgcgg aacagcgcgg
     1201 tcgcacggtt gtcgtcgacc accatgtcct tgtcggccat ggtcagctcg catacgattc
     1261 cggtgcgccg ctgaaactcc ttgacctgcc actcgatcgc cgcggaaaga ccaaggtcca
     1321 gcactgtcgg gcgcaggttg ttgatgatcg cgcgcatagc cttcatcgtg ctgtcgatgt
     1381 ggtccagcac cgcgttcact ttcttgttga gcttcggatg agctgcaccg gtgcgcgcat
     1441 gcagcatcgc cacgtcgatg cgcagtgcaa gcaggttctg cccgagctcg tcatggatct
     1501 cgcgcgcaat gcgcttgcgc tcgtcttcct tgatgcgttc ctggtagctg gccagctggc
     1561 gcagttcttc acgcgactgg cgcagcgctt cctcggcctg gcgccgctcg ttgatgtgct
     1621 ccagatcctg gcgacgctgt tcctgcagcg cctggttcag ctgctccagc tcctgcttct
     1681 ggtgctgcaa ctggaggttc ttcttggcca gttcgacgaa caccgctacc ttcgtctgaa
     1741 gaatctgcgg aatgatcggc gtgaagagat agtcgaccgc gccgcgctga tacgccttca
     1801 gcctgtgcat ttcatcggaa taatgcgcgg tcacgaaaat gatcggcacc gaagcggagc
     1861 ggggatggga gtgaatggct tcggcggtct cgaagccatc catgccgggc atgctgacgt
     1921 cgagcaggat taccgcgaaa tcgtgcttga gcacgtggcg cagcgcctcc tcgcccgagc
     1981 gagcggtgat gacttcgtgc ccctgatcct gggaaagcag actcgccagc gcgagcaggc
     2041 tggcggcatc gtcattgaca agtaggacct ttggcgtaaa cacgggctgg actatctcac
     2101 gaaaagattc cggcaggact gatcttgcgc atgcctgctg cacgccgaac tcgggaacac
     2161 ggccagcggg caggacagca atcggcttga cgaccgacgc gcattaccca tccaaacaaa
     2221 ggggccaatg taacacaatc gaaactgccg ggcagaacgc agcggaaccg ccggttacgc
     2281 gccggggggc gcggaaaagc agctgcttcg ggcggatgcg gtcgtcattg tcgtctgctc
     2341 cgcggtttcc agccacttgc gcacgcgcgc cgcgtctccg agccgcgaat ccttgccgcg
     2401 tgaatcgaga aacaccatga cgaccgggcg tccgtcgacg cgcgcctgca tgagcaggca
     2461 acgccccgcc tcggaaatgt agccggtctt ctgcaagccg atgtcccagt cgggatgatc
     2521 gaccagtcgg ttcgaattca tgtactggag ctgatggccg ccggccgccg cgatcgcatg
     2581 gcgcggatgc gtggaatagt cgcgaatcag cggatggcgg tatgcggcga cggccagcct
     2641 ggttagatcg tgggcggacg ccacgttttc gctggacagg ccggtcgatt cgacgaagcg
     2701 ggtgttgcgc atgccgagtg agcgcgcgcg cgcgttcatg gcggcgacga aggccggcgt
     2761 gccgcccgga tagtggcgcg cgagcgccga tgccgcccgg ttctccgaac tcatcagcga
     2821 cagatgcaag gcttcggcgc gagtcatgcg ggttccgacg cgcagacgcg aactgctgtt
     2881 cttcaggcga tcgatgtcgt cggcggtgat ctcgatcacc t
//
LOCUS       DATLGF010000400        13022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_120827, whole genome shotgun sequence.
ACCESSION   DATLGF010000400 DATLGF010000000
VERSION     DATLGF010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 13022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13022
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120827"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VM406_12230"
     CDS             <1..377
                     /locus_tag="VM406_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HVL76775.1"
                     /translation="KERSGLALPELRRAATVHAHCHHKSVLGTDAETAVLEKLGVDFE
                     MLDSGCCGMAGSFGFEKDKYDVSIACGERVLLPAVRSAAEDTLIIADGFSCREQIVQT
                     TGREPLHLAQVMRMALEGGHGR"
     gene            648..1022
                     /locus_tag="VM406_12235"
     CDS             648..1022
                     /locus_tag="VM406_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008344852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HVL76776.1"
                     /translation="MQPIGKRRWAIAEGMIPSASTSNDPALISHETACILNAGETQAR
                     VRITVFFKDRGPAGPYKLVIPPQRTLHLRFNDLNDPETIPRDTDYASVIESDVPIVVQ
                     HTRLDSRKAELSILSTIAYASD"
     gene            1081..2403
                     /locus_tag="VM406_12240"
     CDS             1081..2403
                     /locus_tag="VM406_12240"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HVL76777.1"
                     /translation="MKRLLGIWRNIQYSLWFIPALIVSFSVLLAVLSIVLDGRVDSGD
                     LPDWPRLFTGSADGARGTLEVIAAAMISIGALTFSITVLVLSSAASTYTPRVIRTFMG
                     STLTKTVLGVFVGVYVYALIVLRTVSSSEPEFVPAIGVTLGLILALVGVGFLIAFIHH
                     MAYSIEVSSIVSTLGRDTVEAARKLFPQEAREAGAEPERASADDSSDTQLAWFPVPAN
                     ATGYIQAIDSPSLARTAGENGVQLKMERAVGEFVIEGEPVMHATQRPDDALMAAVNRC
                     FSIGAQRTVEQDVSVGIRQLADIALRAISASVNDSTTAAMCVDYLTGILAELGRRRLS
                     SETETWEGRRTLRLRCPDFADYLRLAFEQIRQQAGANLAIYLRMLDALETIARAGMHP
                     SRRKAVEREASLVVAYGRAALRHADEQARLEQAWRRTCAAISMTGDGR"
     gene            2400..3131
                     /locus_tag="VM406_12245"
     CDS             2400..3131
                     /locus_tag="VM406_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76778.1"
                     /translation="MNGSVQVWIAVAFMAIVHLLAGHLRFLSYKPRSRWLSAGGGIAV
                     AYAFLHLLPELHGHHRILRQANAPAFSIVQHHAYMVALLGLCAFYGLEHWAKQSRAKN
                     REPGDATTPGVFWISVGAFTLYNALLVYLLAHEGVRDGHELALFTVAVAVHFAVNDHG
                     LYEHHKHLYAYYGRWLLAGGAVLGGVAAHWLDLPDPVMAGMMAFLIGAIVLNVLKEEL
                     PDDRESRFGAFTLGALAYAGLLLAI"
     gene            3236..5491
                     /locus_tag="VM406_12250"
     CDS             3236..5491
                     /locus_tag="VM406_12250"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HVL76779.1"
                     /translation="MYNALPTPLCEAPHARRLAGMAARRLAALVLAIFAGGTAFAATV
                     PSGFVDSRIHSGFESPTALDTLPDGRVLVLQQDGVVHIVKDDALLPEPFYTVPFADPY
                     VETGCMGATHDPAFLTNGHVYFYCTIRQGEQRFNRVVRVTAQGDAAAPGSEQVILDLP
                     PIPPDPVDGSRVAFHVGGAMRFGADGKLYVAVGGHENTHVTPAGDSFSQRLDHPFGKL
                     LRLNHDGSFPEDNPFAGVEGAYPGTYSLGLRNPYTMDIEEETGRIFVNDVGAGSFEEI
                     IDAQPGANYGWPYYEGKVGDARFSDALHIYGHEPDDEGNRRCAVTGGAFYDPTIQQFP
                     SEYLNLYLFADYCSGAIYRIDPQAPDADRIFASGLAGPIGLAVAPDGSLYYLTRHTVG
                     EAGQGDDRGLLGKITYTGSLAPRIARQPQDQLVYVGDAATFSVLAADAQEIQWQRNGV
                     DIPGATAATYTLPQTGTADDGAQFQAVLRNAHGNTTSRPATLRMTTDRLPDVTILRPE
                     EGDRYQPGMTITFSGAATDAEDGQLAPSALTWLANFHHDTHTHPFIPHRRGVDSGTVT
                     VPDFEATTANTWIRFTLRAEDSAGQARSVSRDIFPRFQLSELAPSGVPENGLGPFERD
                     TAYGGVPMALNGVPYPKGIGVLAPSSLSFQLEGICAGRLISDIGLDDSAGEQGSAVFQ
                     VYLDDALVFDSDVVRGGEPRQAINIDVTGTRELRLVVTDAGDGNARDRANWAGARLTC
                     PTLPLATRIGN"
     gene            5720..7237
                     /locus_tag="VM406_12255"
     CDS             5720..7237
                     /locus_tag="VM406_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL76780.1"
                     /translation="MLAASALLAACGGSGSDSQALGGQNVAVLAAEETRHAKHPDAEK
                     GKPKEESREHDGRSFVPADPMAAADYEQAADGGAGFRSLAGAGVQTDRWAGLLDGSLY
                     RIEVPHNWNGKLVMYAHGYAGTGPVLGVTMPSIRRHLIENGYAWAASSYTKNYYDVRA
                     GVEDTNLLANSFKKIAAERGRKLHAPHKTYIVGHSMGGHIAGAAVEEETKKTAVNKTK
                     YDGAVPMCGVMGDTELFDYFAAYQTVAQALAGHVHHPTREFAGIAADVRGKLFTTFST
                     VPTAEGMLLYQVVKHLTGGERPVYAQGFGNTALQNVVWGTFGSDGTVNGILNRNTLDT
                     KRFVYQFDDDPAVSAEEAAFNALVQRLGAAADANRQRIDGLRWIPAVNGDFKVPVVSI
                     HTLGDMYVPFSMQQIYKLRAKANKSDKLLVQRAIRAPSHCDFTVKEQVEAFEAMIAWA
                     EKGIKPGGDEVLDPAAVANPKYGCAYTRNVAGPDDNPVTIATRNAIAPMLAACPS"
     gene            complement(7485..7832)
                     /locus_tag="VM406_12260"
     CDS             complement(7485..7832)
                     /locus_tag="VM406_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HVL76781.1"
                     /translation="MRYNGSCHCGHVAFEVEGEIESAMSCNCSICRRKGSLLWFVPRD
                     ALQLTTPDANARTYMFNQHVIRHRFCPVCGIHPYGEGVDPDGNRIAAVNIRCLENLEL
                     ESVPVTHFDGRSL"
     gene            complement(7853..8962)
                     /locus_tag="VM406_12265"
     CDS             complement(7853..8962)
                     /locus_tag="VM406_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006413019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HVL76782.1"
                     /translation="MNARALLSTRKGLFEASREGGHWSLREAGFAGEPVSCALHDSRD
                     GAVYAALNLGHFGVKLHRREPDTEAWSEIGVPVYPPQQPAQQDAQQKDVEWTLQMIWS
                     LAAGGNDAPGVLWAGTLPGGLFRSADRGTSWELVRSLWDEPSRPEWFGGGYDVPGLHS
                     ICVDPRDSRHLLVGISCGGAWVTRDGGASWKAAVAGMRAAYMPPERAEEPAVQDPHLI
                     AQCRASPDVYWCQHHNGIWRSADGAGRWEEIGPAARAVASDFGFAVAAHPADPLTAWF
                     VPAIADERRIPIDAALCVTRTRDGGKSFEALREGLPQRQCYDLVYRHGLAVAPDGRTL
                     LIGSTTGSVWASGDGGERWELLSAHLPPVYAVTFF"
     gene            complement(8965..9234)
                     /locus_tag="VM406_12270"
     CDS             complement(8965..9234)
                     /locus_tag="VM406_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HVL76783.1"
                     /translation="MPTLIFTQQLRRFIPAPEVTTAAATLRAALEDAFGAHPQLRGYV
                     LDDQGHLRRHLTVFIDGRRLRDHADLSDPLQPASKVHVMQALSGG"
     gene            complement(9371..9670)
                     /locus_tag="VM406_12275"
     CDS             complement(9371..9670)
                     /locus_tag="VM406_12275"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HVL76784.1"
                     /translation="MAYLRTTVGRWNIDLYSAEAGEIFARIASEGLAVFHAQPGFIRY
                     RLMRADASTTVAVAEWESEALGAAGALAYREWLRGSGIAAKLELETYAGEVIVAS"
     gene            9834..10457
                     /locus_tag="VM406_12280"
     CDS             9834..10457
                     /locus_tag="VM406_12280"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HVL76785.1"
                     /translation="MLLILILFSLPARASGLLHIGGCPMRPATECVDMSLIGANMEGM
                     DLSDSLLARTRFLAARLTNAVLDGADLTLAKFQNATLEGASMLSAELWAANLSDANLR
                     GAALRGANMEKAKLRRTDLRDADLSGARLLMAELPHAQLQEANLEGVDLRGANLIGAD
                     LNYANLRGADLSYANLTNTDLDGADLRGTTFVGAITTGCRGCPLILN"
     gene            complement(10450..10905)
                     /locus_tag="VM406_12285"
     CDS             complement(10450..10905)
                     /locus_tag="VM406_12285"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HVL76786.1"
                     /translation="MTRIKRLHTAGRKSAPPAASQAPSTGAAGFKSTGGLGRILRATR
                     YSLSGLRAAWRVEAAFRQEVVLGVAMLVLVPWLAPSLVFGVLMAGSILLVWGMEMLNS
                     AIEALADAISSEHHPLLGRAKDLGSAAVMFCLLLCALVWGAALYLRFFS"
     gene            complement(10962..11336)
                     /locus_tag="VM406_12290"
     CDS             complement(10962..11336)
                     /locus_tag="VM406_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76787.1"
                     /translation="MKKLSTLCILAALIAPFPAVAAQPVERGGARAADADSAGEPRDP
                     SAFSYGEYHRSSKMTPGGSQGPEVWGEERTDVGPPLPASGAQARGGQAAGSDEPRDAR
                     ADERGRGRDGGSRGRGEEADKD"
     gene            complement(11411..11929)
                     /locus_tag="VM406_12295"
     CDS             complement(11411..11929)
                     /locus_tag="VM406_12295"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HVL76788.1"
                     /translation="MMLFEGWGSIFRVLMVGSLAYAGLVILLRVSGNRTLSKMNSFDL
                     VITVAFGSALSTILTSADLALATGMAAIGLLVMLQFAITWLSVRSRGLSKTVKTAPSL
                     LLENGAFRPDALRRTRVTEDEVRSAVRQHGYGDLERIAAVVMESDGSLSVISKQDLGS
                     GSALEGLPRQAN"
     gene            complement(11926..>13022)
                     /locus_tag="VM406_12300"
     CDS             complement(11926..>13022)
                     /locus_tag="VM406_12300"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HVL76789.1"
                     /translation="GYHPHPPASPRQRALLRRILMHYAIAGVFILVLGLLWFAASVLL
                     LLFSAILIAVLLTSVAGKLRQWLHLRYGYALALVLLLFVTVIGTSVWLLAPSVIEQGE
                     QLSQTLPNAIQRARDFLLQLPMLQGLVNEFPDLDRAAPDFDALLSRAGSVFTGVFGVV
                     ANLVIVLFVSVYLAAQPQVYVSGFVTLFPKSARPRICEVLYRIGDTLGLWLIGKMISM
                     VIVGAVTATGLTLLDVPLALVLGLVAGLFEFIPYLGPILAGVPAILIAFAHDPTLALY
                     VLFLFIGIQIFEGYFVQPLIERRMVSLPPAMTITMQVLLALPFGLLGVALATPLTAAI
                     AVVITMLYVQDVLGDPVHPPGGSNDSEGDE"
BASE COUNT         2332 a         4484 c         4164 g         2042 t
ORIGIN      
        1 agaaggaacg gagtggactg gcgctgcccg aactgcggcg cgccgcgacc gtccatgcgc
       61 actgccacca caagtcggtg ctgggcaccg atgccgagac ggccgtgctg gaaaagctcg
      121 gcgtcgactt cgagatgctc gattccggct gctgcggcat ggccggttcg ttcgggttcg
      181 agaaggacaa gtacgacgtg tcgatcgcct gcggcgaacg cgtgctgctg ccggccgtac
      241 gatcggccgc ggaagatacc ttgatcattg ccgacggctt cagctgccgc gagcagatcg
      301 tgcagaccac cggccgcgag ccgctgcacc tggcgcaggt gatgcgcatg gcgctggagg
      361 gcgggcatgg acgctgaggt gcgcgcggca cggtcattcg cccccaccgt cgcgccgccg
      421 aaagccgggg cccagcgtcg ttcgccccgc gcaccgacac gccctgttgc tcgcctcggc
      481 aagcccccgc gcggccgggg agcccggttc gcgggcacgc agccctgcac gcgttcgaac
      541 tgggttcccg cctgcgcggg aacgacattg cacgggggcg tgcccgaccg ggaaagcggc
      601 gcgacacccc aaccacctgc gcttgaccag cacaacaagg agaaccgatg caaccaatag
      661 gaaaacgccg ctgggcgatt gccgaaggga tgattccttc cgcgagcacc agcaatgatc
      721 ccgccctgat cagccatgaa accgcctgca tcctcaatgc cggcgagacg caggcgcgcg
      781 tgcgcatcac ggtgttcttc aaggaccgcg gaccggcggg cccatacaag ctggtgattc
      841 cgccgcagcg cacgctgcac ctgcgcttca acgacctgaa cgaccccgaa acgattccgc
      901 gcgacaccga ttatgcgagc gtgatcgagt ccgacgtgcc gattgtcgtc cagcacacac
      961 ggctcgacag tcgcaaggcg gaactgagca ttctgtcgac gatcgcctac gcgagcgact
     1021 gaagatggcg cgtcgatcgc tgcttcaacc gcggggaccg gtcttgcccg ctgcacggcg
     1081 atgaaacggc tgctcgggat ctggcgcaat attcaataca gcctctggtt cattccggcg
     1141 ctgatcgtct ccttttccgt attgctggcc gttctctcga tcgtgctcga cgggcgcgtc
     1201 gacagcggcg accttcccga ctggccgcgc ctgtttaccg gcagcgccga tggggcgcgc
     1261 gggacgctgg aagtgatcgc cgccgcgatg atcagcatcg gcgccctcac cttttcgatc
     1321 acggtgctgg tgctttccag cgccgcctcg acctacacgc cgcgcgtcat ccgtaccttc
     1381 atgggcagca cgctgaccaa gacggtgctg ggcgtgttcg tcggcgtcta tgtgtatgcg
     1441 ctgatcgtgc tgcgcacggt cagcagcagc gagcccgaat tcgttccggc gatcggtgtc
     1501 acgctcgggc tcatccttgc gctggtcggg gtcggattcc tgatcgcgtt catccaccat
     1561 atggcgtact cgatcgaggt ctcaagcatc gtctccacgc tcggccgcga taccgtggag
     1621 gccgcgcgca agctgttccc gcaggaagcg cgggaggccg gcgctgaacc cgagcgcgca
     1681 tcggcagacg attccagcga cacacagctc gcctggttcc cggtgcctgc caatgcaacc
     1741 ggatacatcc aggccatcga ttcgcccagt cttgcacgca cggccggcga gaacggcgtg
     1801 cagttgaaga tggaacgcgc ggtcggggaa ttcgtgatcg agggcgaacc cgtcatgcat
     1861 gccacgcagc ggccggatga cgcgctgatg gccgcggtca accgctgctt ttcgatcggc
     1921 gcgcaacgga ccgtcgaaca ggatgtctcc gtcggaatcc gccagctcgc cgacattgca
     1981 ctgcgggcaa tctcagcatc agtcaacgac agcacgacag ccgccatgtg cgtcgattac
     2041 ctgaccggca tcctcgccga acttggccgg cggcgcctga gctcggaaac tgaaacctgg
     2101 gagggacgcc gcaccctgcg cctgcgctgc ccggattttg ccgactatct tcgcctggcc
     2161 ttcgagcaga tccgccagca ggccggcgcc aacctggcga tctacctgcg catgctggac
     2221 gcgctggaaa ccattgcgcg cgcgggcatg catccgagcc gccgcaaggc ggtcgagcgc
     2281 gaagcctcgc tggtcgtggc ctatggacgc gcagcgctgc gccacgccga tgaacaggca
     2341 cgcctggaac aggcctggcg acgcacctgc gcggccatca gcatgaccgg agacggccgt
     2401 tgaacggcag cgtgcaggtc tggatcgcgg tggccttcat ggcgatcgtc cacctgctcg
     2461 ccggccatct gcgctttctt tcctacaagc cgcgcagccg ctggctgtcg gccggcggcg
     2521 gcattgcggt cgcgtatgcg ttcctgcact tgctgcccga gctgcatggg caccaccgca
     2581 tcctgcgcca ggcgaacgcg ccggcattct ccatcgtcca gcaccatgcg tacatggttg
     2641 cgctgctcgg gctgtgcgcg ttctacggac tcgagcactg ggccaagcaa tcgcgcgcga
     2701 agaaccgcga gccgggcgac gccacgacac ccggtgtgtt ctggatcagc gtcggcgcct
     2761 tcacgctcta caacgcgctg ctggtctacc tgcttgcgca cgaaggtgtg cgcgacgggc
     2821 acgaactggc gctgtttacg gtcgccgtcg cggtccattt cgccgtcaac gatcatggcc
     2881 tgtacgagca ccacaagcat ctgtacgcct actatggccg ctggctgctg gccggaggtg
     2941 ccgtgttggg cggcgtggcc gcgcactggc tggaccttcc ggatccggtg atggccggca
     3001 tgatggcctt cctgattggc gcgatcgtcc tcaatgtgct caaggaggaa ctgcccgacg
     3061 accgcgaaag ccgcttcggc gcctttaccc tcggcgcgct tgcctacgcc ggcctgttgc
     3121 tggcgatctg aaacgattcc acgaccggac gctgacgccg gtcaaactgt gttccgtgcg
     3181 gattggcggc ggcacggggg ctctctactc ttcaagtgga atcatcgaag acagcatgta
     3241 caacgcgctc cccaccccgc tttgcgaagc gccgcacgcg cgacgcctcg ccggcatggc
     3301 ggcgcgccgg ctggccgcgc tcgtgctggc aatatttgcg ggcggaaccg cattcgctgc
     3361 cacggtgcct tccggcttcg ttgacagccg gatccacagc ggctttgaaa gtcccaccgc
     3421 gctggatacg ttgccggacg gccgcgtgct ggtgttgcag caggacggcg ttgtgcacat
     3481 cgtcaaggac gatgcgctgc tgcccgaacc gttctacacg gtcccgtttg ccgacccgta
     3541 cgtcgagacc ggctgcatgg gcgcgaccca cgatccggca ttcctgacga acggccacgt
     3601 ctacttctac tgcacgatcc ggcagggcga gcagcgcttc aaccgcgtcg tgcgcgtgac
     3661 ggcgcaaggc gatgctgccg ctcccggcag cgaacaggtg atcctcgacc tgccgccgat
     3721 tccgcccgat ccggtcgatg gctcgcgcgt ggccttccat gtcggcggcg cgatgcgctt
     3781 cggcgcggac ggcaagctgt acgtggccgt cggcgggcac gagaacaccc atgtcacgcc
     3841 ggccggcgac tcgttctccc agcgcctcga ccacccgttc ggcaagctgc tgcggctgaa
     3901 ccacgacggc agcttccccg aggacaatcc cttcgccggc gtcgagggtg cttatcccgg
     3961 cacctacagc ctgggtttgc gcaacccgta cacgatggac atcgaggaag aaaccgggcg
     4021 catcttcgtc aacgacgtgg gcgcgggcag cttcgaggag atcatcgatg cgcaacccgg
     4081 tgccaactac ggctggcctt actacgaggg caaagtcggc gacgcgcgct tttccgatgc
     4141 gctccatatc tacggtcacg agccggacga cgagggcaac cggcgctgcg cggtgaccgg
     4201 cggcgcattc tacgatccga caatccagca gttcccgtcg gaatacctca acctctacct
     4261 gttcgccgac tattgcagcg gcgcgatcta ccggatcgac ccgcaggccc cggacgcgga
     4321 cagaattttt gccagcgggc ttgccggccc gatcggcttg gccgttgcgc ccgacggatc
     4381 gctctactac ctgacgcgcc acacggtcgg ggaagccgga cagggcgacg accggggcct
     4441 gctcggcaag atcacgtaca ccggttcgct tgccccgcgc atcgcaaggc agccgcagga
     4501 ccagctcgtt tatgtcggcg acgccgccac cttcagcgtg ctggcggcgg acgcgcagga
     4561 gatacagtgg cagcgcaacg gcgttgacat ccccggggcg accgctgcca cctataccct
     4621 gccgcagacc ggcaccgccg acgacggcgc gcagttccag gcggtgctgc gcaacgccca
     4681 tggcaacacc acgtcgcgcc cggccacgct gcgcatgacg accgaccgcc tgcccgacgt
     4741 gacgatcctg aggcccgagg aaggcgaccg ctaccagccg gggatgacga tcacgttttc
     4801 gggcgcggcg acggacgccg aggatggcca gcttgcgccc tcggcgctca cctggctggc
     4861 gaacttccac cacgacaccc acacgcatcc gttcatcccg caccggcgcg gcgtggacag
     4921 cggcaccgtc actgtgcccg acttcgaagc caccacagcg aacacctgga tacgcttcac
     4981 gctgcgggcc gaggattcgg cgggccaggc acgctcggtc tcgcgcgaca tcttcccgcg
     5041 cttccagctc agcgaactcg ccccttcggg cgtgccggag aacggtctcg gcccgttcga
     5101 gcgcgacacg gcctacggcg gcgtgccgat ggcgctgaac ggcgtaccgt atccgaaagg
     5161 catcggcgtg ctcgcaccat ccagcctgag ctttcagctc gaaggcatct gcgccgggcg
     5221 gttgatcagt gacatcggac tggatgactc ggccggcgag caggggtcgg ccgtgttcca
     5281 ggtctacctc gatgacgcgc tggtcttcga cagcgacgtg gtgcgcggcg gcgagccgcg
     5341 ccaggcgatc aacatcgacg tcaccggtac gcgcgaactg cggctggtcg tcaccgacgc
     5401 cggagacggc aatgcccgcg accgcgcaaa ctgggccggc gcccgcctga cctgcccgac
     5461 actcccgctg gccacgcgca tcggcaactg atgcgcgcgc cgtgccggcg cccactccct
     5521 tcccgtcgct tcaaatgacc gcaagcaacg gttgttgaac gaactcaacg gatttccaca
     5581 ccgcctcttt tccttgcacg ttttgtcata tttttaattt tccgcacgac cgttcttgaa
     5641 gcggagcacg gcggtcattc ccgacaaaac gacaaggaga caggcagtga agaacaatgg
     5701 aaacttcgcc accccgctga tgcttgccgc cagcgccctg ctcgccgcct gcggcggttc
     5761 cggcagcgac agccaggcgc tgggcggcca gaatgtcgcg gtcctcgccg ccgaggagac
     5821 ccggcacgcc aagcacccgg atgccgagaa aggcaagccc aaggaagaaa gccgcgagca
     5881 cgacggacgc agcttcgtgc cggccgaccc gatggccgcc gcggattacg agcaggccgc
     5941 ggatggcggt gccgggttca ggtcgctggc cggcgccgga gtgcaaaccg accgctgggc
     6001 cgggctgctc gacggctccc tctaccgcat cgaagtgccg cacaactgga acggcaagct
     6061 ggtcatgtac gcgcacggct acgccggcac cggaccggtg ctgggcgtga ccatgccgtc
     6121 gatccgccgc cacctgatcg aaaacggtta tgcatgggcg gcatcaagct acacgaagaa
     6181 ctactacgac gtgcgtgccg gggtcgaaga cacgaacctg ctggccaaca gcttcaagaa
     6241 gatcgccgcc gaacgcggcc gcaagctcca cgcgcctcac aagacctaca tcgtcggcca
     6301 ttcgatgggc gggcatatcg ccggtgctgc ggtggaggag gaaaccaaga agaccgctgt
     6361 caacaagacg aagtacgacg gcgcggtgcc gatgtgcggc gtgatgggcg acaccgagct
     6421 gttcgactac ttcgccgcct accagaccgt cgcgcaggca ctggccggcc atgtgcatca
     6481 cccgacccgc gagttcgccg gcatcgccgc cgacgtgcgc ggcaagctgt tcacgacatt
     6541 cagcaccgtg cctaccgccg aaggcatgct gctgtaccag gtggtcaagc acctgaccgg
     6601 gggcgagcgc ccggtttatg cgcagggctt cggcaacacc gcgttgcaga acgtggtctg
     6661 gggcaccttc ggcagcgacg gcacggtcaa cggcatcctg aaccgcaaca cgctcgatac
     6721 gaagcgcttc gtctatcagt tcgacgacga cccggccgtc tcggccgagg aagccgcgtt
     6781 caacgccctg gtgcaacgcc tgggcgccgc cgccgatgcc aaccgccagc gcattgacgg
     6841 cctacgctgg atccctgccg tcaacggcga tttcaaggtg ccggtggtgt ccatccacac
     6901 cctcggcgac atgtatgtgc cgttcagcat gcagcagatc tacaagctgc gcgcgaaggc
     6961 caacaagagc gacaagctgc tggtgcagcg ggcgatccgc gcgcccagcc attgcgactt
     7021 cacggtgaag gaacaggtcg aagccttcga ggcgatgatt gcctgggccg agaaaggcat
     7081 caagccgggc ggcgacgagg tactcgatcc ggccgccgtc gccaatccga agtacggctg
     7141 tgcgtatacc cgcaacgtcg cgggcccgga cgacaatccg gtgacgatcg ccacccgcaa
     7201 tgcgatagcg ccgatgctgg cggcctgtcc ttcgtagctg cgcaatgcct gtccgcggtg
     7261 ccggcgaacg gcggcgccgc gggcggacga gatacctgac gcgggccgcc ctcactgcgt
     7321 tcgggacgac agcgatcagg cgatggcccc caaacctgtc gttccgagca aagcgaggaa
     7381 cctcatccgt tcgctttgca tcgttgcttg ccaagcggat gaggtccctg acgcgggccg
     7441 ccctcactgt gttcgggacg acagaaacca ggcgatagcc cccatcacag cgaccgcccg
     7501 tcaaaatgcg tcacaggcac ggattcgagt tcgagatttt ccaggcagcg aatgttgacg
     7561 gcggcgatcc ggttgccgtc ggggtcgaca ccctcgccgt acggatgaat gccgcagacc
     7621 ggacagaagc ggtgcctgat cacgtgctgg ttgaacatgt aggtgcgcgc gttcgcatcg
     7681 ggtgtcgtca gctgcagcgc atcgcgcggc acgaaccaga gcagcgaacc cttgcgccgg
     7741 cagatcgagc agttgcacga catagcggac tcgatctcgc cttcgacttc aaacgccaca
     7801 tggccacagt ggcagcttcc gttgtatcgc attctcctct cccatcgtcc gttcagaaaa
     7861 acgtcaccgc atagaccggg ggcaggtgcg ccgacagcag ttcccagcgt tcgccgccgt
     7921 ccccggacgc ccacaccgaa ccggtcgtcg aaccgatcaa gagcgtgcgc ccgtccggcg
     7981 cgaccgccag accatgccga tagacgaggt catagcattg ccgctgcggc agcccctcgc
     8041 gcaaggcttc gaagctcttg ccgccgtcgc gcgtgcgcgt gacgcacagt gccgcatcga
     8101 tcggtatgcg gcgctcgtcc gcgatggccg ggacgaacca cgcggtgagc ggatcggccg
     8161 gatgcgcggc cacggcaaat ccgaaatcgc tggcgaccgc tcgcgcagcg ggtccgattt
     8221 cttcccagcg cccggcgccg tcggccgagc gccagatgcc gttgtggtgc tggcaccagt
     8281 agacgtccgg actggcgcgg cactgcgcga tcaggtgcgg gtcctgcacc gccggctctt
     8341 ccgcccgctc aggcggcatg taggccgcgc gcattcccgc caccgccgcc ttccacgacg
     8401 ccccgccgtc gcgcgtgacc caggcgccgc cgcaggagat gccgaccagc agatggcggc
     8461 tgtcacgcgg gtccacgcag atcgaatgga ggcccggaac gtcgtagccg ccgccaaacc
     8521 actccggccg cgacggctcg tcccacaggc tgcgcaccag ttcccagctt gtgccgcggt
     8581 cggcggagcg gaacaggccg cccggcagcg tgccggccca cagcacgccg ggcgcgtcat
     8641 tgccgccggc ggccagcgac cagatcatct gcagcgtcca ctccacatcc ttctgttgcg
     8701 cgtcttgttg tgcaggctgc tgcggtggat acaccggtac gccgatctcg ctccacgcct
     8761 cggtgtctgg ctccctgcgg tgcagcttga cgccgaagtg gccgaggttc agcgcggcat
     8821 agaccgcgcc gtcgcggctg tcatgcagcg cgcacgatac cggctcgccg gcaaacccgg
     8881 cctcgcggag gctccagtgg ccgccttcgc ggctggcttc gaacaggccc ttgcgggtcg
     8941 acaacagcgc acgcgcgttc atcctcatcc tcctgagagt gcctgcatga catggacctt
     9001 gctggcaggc tgcagcggat cggacaggtc cgcatggtcg cgcagccgcc tgccgtcaat
     9061 gaaaaccgtc agatgccggc gcagatgccc ctggtcgtcg agcacgtagc cgcgcagctg
     9121 cggatgcgcg ccgaacgcgt cctccagcgc ggcgcgcagg gtcgccgccg ccgtcgtcac
     9181 ttccggcgct ggaatgaaac gtcgcaactg ctgggtgaag atcaaagttg gcatgcggca
     9241 cgcgcttcat ggaagtggtc ggacccgacg ggccgtgggc gctggcaaat gcacctcgca
     9301 ccatccggac agggatagtc ggtcgggaac cctccagatc gacatcggca cgcatttacc
     9361 ggcatgccac tcaggacgcc acgatcacct cgcccgcata ggtctccaac tcgagcttcg
     9421 cggcaatgcc gctgccgcgc agccattcgc ggtatgcaag cgcaccggcc gcgccaagcg
     9481 cctcgctctc ccattcggcg acggctaccg tcgtggacgc atcggcccgc atgaggcggt
     9541 agcgaatgaa acccggctgc gcgtggaaca cggcaaggcc ctcgctcgcg atccgcgcaa
     9601 agatttctcc cgcctcggcg gagtagagat cgatgttcca gcgtccgaca gtggtgcgca
     9661 gataggccat ggcatgtgct cgacgtccgt tccgcttcct gcaccgctca agacgcccgt
     9721 gccgcagcga ttcggcgcac ggcatgaagt ttgcgtggcc cgatgcaagc gatgaacctt
     9781 gtccgaggtt ccagactaga tatgggaaag gacttgtgca acgccttggc tgcatgcttt
     9841 tgatcctgat tctgttcagc ctgccggccc gtgccagcgg cctgctgcac ataggcggct
     9901 gcccgatgcg gcccgccacc gaatgcgtgg acatgtcgct gatcggcgcc aacatggagg
     9961 gcatggatct gtccgactcg ctgctggcga gaacgcgctt tctggccgcg cgcctcacta
    10021 acgcggtgct cgacggcgcg gacctgacgc tggcgaagtt ccagaacgcc acgctggagg
    10081 gggcgtcgat gctgtctgcg gaactgtggg cggccaatct cagcgacgcg aacctgagag
    10141 gcgcggcctt gcgcggagcg aacatggaga aggcaaaact gcgccgtacc gacctgcgcg
    10201 atgcggacct gtccggcgcg cgcctgctga tggcggaact gccgcacgcg cagttgcaag
    10261 aggccaatct tgaaggggtg gacttgcgcg gcgcaaacct gatcggcgcc gacctgaact
    10321 acgcgaacct gcgcggcgcc gatctttcgt acgccaacct gaccaacacc gacctcgacg
    10381 gcgcagacct gcgcggcacg accttcgtgg gcgcaatcac caccggctgc cgcggctgcc
    10441 cgctgatcct caactgaaaa agcgcagata cagcgccgcg ccccagacca gcgcgcacaa
    10501 cagcaggcaa aacatcaccg ccgcgctgcc gaggtctttt gcgcgcccga gcagcggatg
    10561 gtgttcggaa gagattgcat cggccagcgc ctcgatcgcc gagttgagca tctccatgcc
    10621 ccacaccagc aggatggatc cagccatcag caccccgaac accagcgatg gcgcgagcca
    10681 gggaaccagc accagcatgg cgaccccgag cacgacttcc tgtcgaaatg cggcctcgac
    10741 ccgccacgcc gcgcgcagac ccgacagcga atagcgggtt gcacgcagga tgcgacccag
    10801 cccgccggtg ctcttgaaac ccgccgctcc ggtggaaggc gcttgcgacg cagcgggagg
    10861 ggcggatttg cggccggcgg tatggagtcg cttgatgcgg gtcatggctg tcgaaaatgc
    10921 ggggcggacc agcatcctac acgcaagcga tccccggcag gtcaatcctt gtccgcttcc
    10981 tctccccgcc cccggctgcc gccgtcgcgc ccgcggccgc gctcgtcggc ccgcgcatct
    11041 cggggctcat cggagccggc ggcctgccct ccgcgggcct gcgcgccgct ggccggaagc
    11101 ggcgggccca cgtcggtacg ttcctcgccc cacacttccg gcccttgcga gccgccgggg
    11161 gtcatcttgc tcgagcgatg gtattcgccg taggaaaacg cggacgggtc gcgcggttca
    11221 cccgccgagt ccgcgtccgc tgcgcgcgct ccgccgcgct cgaccggctg cgcagccacg
    11281 gcgggaaacg gggcaatgag cgcggcaaga atgcaaagcg ttgacaattt tttcatggct
    11341 tctcctgttg ccggcgcccg ctttgctcac gggcgttcca tgcacccggt tgttgcacga
    11401 aagatgccag tcagttcgct tgccggggga gtccctccag cgcggaaccg gatccgaggt
    11461 cctgcttcga tatcactgac aagctgccgt cgctttccat gacgaccgcg gcgatacgtt
    11521 ccaggtcgcc gtatccatgc tgccggacag cggatcgcac ctcatcctcg gtgaccctcg
    11581 tccggcgcaa cgcgtccgga cggaacgcgc cgttctccag caacaggctg ggcgcggtct
    11641 tgacggtctt gctgaggccg cgcgaacgca ccgaaagcca ggtgatggca aactgcagca
    11701 tgacgagcag accgatcgcc gccatgcccg tcgcaagggc gaggtccgcg ctggtcagga
    11761 tcgtcgacag ggccgaaccg aacgcgacgg taatcacaag atcgaaggaa ttcatcttcg
    11821 acagcgtgcg attgccgctc acgcgcagca ggatgaccag gccggcgtaa gcgagcgatc
    11881 ccaccatcag cacccggaaa atgcttcccc atccctcgaa cagcatcatt catccccttc
    11941 cgaatcgttg ctcccgccgg gcggatgcac cggatcaccg agcacatcct gcacgtacag
    12001 catcgtgatg accacggcaa tggcggccgt cagcggggtg gcgagcgcga cgccgagcag
    12061 gccgaacggc agcgccagca gcacctgcat cgtgatcgtc atcgccggcg gcagcgaaac
    12121 catgcgccgc tcgatcagcg gctgcacgaa atagccctcg aagatctgga tcccgatgaa
    12181 cagaaacagc acgtacagcg ccagcgtcgg atcatgcgcg aacgcgatca gtattgcggg
    12241 cacgccggcc aggatgggac cgaggtaggg aatgaactcg aacaggcctg ccaccagtcc
    12301 cagcaccagc gccagaggca cgtcgagcag ggtcaggccg gtggcggtga cggcgccgac
    12361 gatcaccatc gagatcattt tcccgatcag ccacagaccg agcgtgtcgc caatgcggta
    12421 aagcacctcg cagatgcgcg ggcgcgccga tttcgggaac agggtgacga atccgctgac
    12481 atagacctgc ggctgggccg ccaggtagac cgagacgaac aggacgatga ccagattggc
    12541 gacgacgccg aacacgccgg taaacacgct gccggcgcga ctgagcagcg catcgaaatc
    12601 cggcgccgcc cggtccaggt ccgggaattc gttgaccagc ccctgcagca tcggcaattg
    12661 cagcagaaaa tcgcgtgcac gctggatcgc attgggcagc gtttgggaaa gctgctcgcc
    12721 ctgctcgatc acgctcggcg cgagcagcca cacgctcgtg ccgatgaccg tgacgaacag
    12781 cagcagcacg agggccagcg cataaccgta gcgcaggtgc aaccactgcc taagcttccc
    12841 ggccacactg gtcagcagaa cggcgatcag gatcgccgag aacagcagca gcagcacgct
    12901 cgcggcaaac cagagcagcc cgagcacgag gatgaacacg cccgcgatcg cgtagtgcat
    12961 caggatgcgg cgcagcagcg cgcgttgtcg cggcgacgcg gggggatgcg gatggtagcc
    13021 gc
//
LOCUS       DATLGF010000401         4024 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_62736,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000401 DATLGF010000000
VERSION     DATLGF010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62736"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VM406_12305"
     CDS             <1..259
                     /locus_tag="VM406_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HVL76790.1"
                     /translation="YAAARWIYRNRDRRGVRAFKYGMAPIVVALIVATGWILASVNNV
                     PQRDWPFWLLTAGTAALVLRTRLHLLWMLGTGALVGALWLG"
     gene            complement(266..1744)
                     /gene="zwf"
                     /locus_tag="VM406_12310"
     CDS             complement(266..1744)
                     /gene="zwf"
                     /locus_tag="VM406_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HVL76791.1"
                     /translation="MPPIPASPAALPPSTPLDMIIFGGAGDLSIRKLLPALYMAHLHG
                     NLPPATRIIAVGRHDYGREGYIEFVQKQSQAFIDAKNLEPQAWEQFLQLLDYVSLDVT
                     DSAGYAELARHSRPGVERVFYLATAPTLFTSICSNLAGCGLVDPASRVVLEKPLGHDL
                     ASAREINDAVGRYFSESQIYRIDHYLGKETVQNLMVLRFGNGILEPLWRTPNIESVQI
                     TVAETVGVGSRAGFYDGTGALRDMVQNHLLQLLCIIAMEPPVSLEPDAVRDEKLKVLR
                     SLRKMTLGDIARDTVRGQYGVGAVNGEAVKAYRDEAGVPPDSRTETFVALRAHVDTWR
                     WAHVPFFLRTGKRMQKRMSQIVIDFADPPFSIFPDAPKTRANRLVIQLQPEESIQLQL
                     MAKQPGSGMQMLPVNLNLDLQSAFSQRRAEAYERLLIDVVRGRLTHFMRRDELEAAWA
                     WVDPILEGWKELGERPLPYMAGSWGPDASAALLARQGLGWIE"
     gene            1921..2823
                     /locus_tag="VM406_12315"
     CDS             1921..2823
                     /locus_tag="VM406_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HVL76792.1"
                     /translation="MKIIVPPRGASSTNPWITPSLAVAAVLATLLLYLVLQRQFQAYD
                     SRIALAFQASSIAALATAAGTLPLLFVKAFSQRANDTMLGFGAGVMLAAAAYSLVLPG
                     LEAASEQIGGKWTPAVVVGIAIVFGAGLLMATEWLVPHEHFIKGREGRDGRAIRRAWL
                     FVFAIALHNIPEGLAIGVAFAGTDLGSAWALATGISIQNVVEGLVTAMAIRSVGYSRT
                     LSMSLGAASGLVEPAAAVFGAFLIGLSAELLPWGLGFAAGAMLFVISHEVIPESHRKG
                     HESFATGGLMIGFVLMMALDTVLA"
     gene            complement(2942..>4024)
                     /locus_tag="VM406_12320"
     CDS             complement(2942..>4024)
                     /locus_tag="VM406_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HVL76793.1"
                     /translation="IHILVVDDFPQNLVANEAILARPGLKVLKAASGFEALELLLEHE
                     VALALVDVQMPGMDGFELAEMMRGNQRTSAIPIIFMTAAAEEATRSFRGYQAGAVDFL
                     TKPVNPDVLRGKVEVFVELQAQKNQIREQVEELRRALQMNELFIAVLGHDLRTPLSAV
                     LHGAELLKHIGGDARVEETAERIHASGTRMEKMVRQLLDVAQTRAAGVTLFKSKANLE
                     DVCRRIIAEIEHRASASRIRIESSGKLDGLFDTDRMSQVISNLLGNALQHGRDGTPIR
                     IELDGTDAGTIRLRLTNEGVIPPETLDGIFKPFYFPDRKRTPSAGLGLGLYIVHEFVV
                     AHGGTVSVHSSPWEGTTSFEVALPRQ"
BASE COUNT          616 a         1287 c         1392 g          729 t
ORIGIN      
        1 ctatgcggcg gcacgctgga tctaccggaa ccgggaccgg cgcggcgtgc gcgccttcaa
       61 gtacggcatg gcgcctatcg tcgtcgcgct gatcgtcgcg accggctgga tcctcgccag
      121 cgtcaacaat gttccacaac gcgactggcc gttctggctg ctgacggccg gcaccgcggc
      181 gctggtgctg cgcacccgcc tgcacctgct gtggatgctc ggcacaggag cgctggtcgg
      241 cgcgctctgg ctagggtagg cgccgctatt cgatccagcc cagtccttgc cgcgcgagca
      301 gcgcggccga cgcgtccggc ccccagctgc cggccatgta gggcaacggg cgctcgccca
      361 gttccttcca tccttcgagg atcgggtcaa cccaggccca tgcggcttcc agttcgtcgc
      421 gccgcatgaa gtgcgtgagc cggccgcgga ccacgtcgat cagcaggcgt tcgtaggctt
      481 cggcgcggcg ctgcgagaat gccgattgca agtccagatt caggttcacc ggcagcatct
      541 gcatgccgct gccgggctgc ttggccatca gctgcagctg gatcgactcc tccggctgca
      601 gctggatcac caggcggttc gcgcgcgtct tcggcgcatc agggaagatc gagaacggcg
      661 gatcggcaaa gtcgatcacg atctgcgaca tccgcttctg catgcgcttg ccggtgcgca
      721 ggaagaacgg cacgtgcgcc cagcgccagg tgtccacgtg ggcgcgcagc gcgacgaagg
      781 tttcggtgcg gctgtccggc ggcacgccgg cctcgtcgcg gtaggccttg accgcctcgc
      841 cgttgaccgc gccgacgccg tactggccgc gcaccgtgtc gcgcgcaatg tcgccgagcg
      901 tcatcttgcg cagcgagcgc agcaccttca gcttttcgtc gcgcaccgca tccggctcca
      961 gcgagaccgg gggttccatc gcgatgatgc acaacagctg cagcagatgg ttctgcacca
     1021 tgtcgcgcag cgcgccggtg ccgtcgtaga agccggcgcg gctgcccacg ccgacggtct
     1081 ccgccaccgt gatctgcacg ctctcgatat tgggcgtgcg ccacagcggt tcgaggatgc
     1141 cgttgccgaa gcgcagcacc atcaggttct gtacggtttc cttgccgagg taatggtcga
     1201 tgcggtagat ctgcgattcg ctgaagtagc ggccgaccgc gtcattgatc tcgcgcgccg
     1261 acgccaggtc atggccgagc ggtttctcca gcaccacgcg gctggccggg tcgaccagcc
     1321 cgcagccggc caggttgctg cagatgctgg taaacagcgt cggggcggtc gccaggtaga
     1381 acacgcgctc cacgccgggg cgcgaatgcc gggccagttc cgcgtagccg gcactgtcgg
     1441 tgacgtcgag cgacacgtag tcgagcagtt gcaggaattg ctcccaggcc tgcggttcca
     1501 ggttctttgc gtcgatgaaa gcctgggact gtttctgcac gaattcgata tagccttcgc
     1561 gcccgtaatc gtggcggccg actgcgatga tgcgcgtggc cggcggaaga ttgccatgca
     1621 ggtgggccat gtagagcgcg ggcagcagct tgcggatcga caagtcgccg gcgcctccga
     1681 agatgatcat gtcgagcgga gtggacggcg gcagggcggc aggagaggct ggaatgggcg
     1741 gcatgggcgg caatcgagga ctggtccgga tttgtcgagg aatggaatcg gtattgtagt
     1801 gtgcgcgatc cccgcgtgtc cccggatgag actggtatgc aacttgctgc gggaccagcg
     1861 cgcggatggc cgcgtccggc ggcatgcata gcttccctcg ctcttcaacg acttgtccga
     1921 atgaaaatca ttgtgccccc gcgcggcgca tcaagcacga atccctggat cacgccatcg
     1981 cttgcggtgg cggcggtgct cgccacgcta ctgctctatc tggtgctgca gcgccagttc
     2041 caggcctacg acagcaggat cgcgctggca ttccaggcaa gctcgatcgc agcgctcgcc
     2101 acggccgccg gcacgctgcc gctgctgttc gtcaaggcgt tttcccagcg ggcgaacgac
     2161 acgatgctgg ggttcggcgc cggggtgatg ctggccgcgg ccgcctattc gctggtgctg
     2221 cccgggctgg aggcggccag cgaacagatc ggcggcaagt ggacgcccgc cgtcgtggtg
     2281 ggcattgcga tcgtgttcgg agcagggctg ctgatggcga ccgagtggct ggtcccgcac
     2341 gagcacttca tcaaggggcg cgaaggccgg gacggacgcg cgatccgccg cgcctggctg
     2401 ttcgtgttcg cgatcgccct gcacaacata ccggaaggac tggcgatcgg cgtggccttc
     2461 gcgggcaccg atctcggcag tgcatgggcg ctggcgaccg gcatttcgat ccagaacgtg
     2521 gtggagggcc tggtgaccgc gatggcgata cgcagcgtcg gctacagccg cacgctgtcg
     2581 atgagcctgg gcgccgcgtc cggattggtg gaacccgcgg cggcggtatt cggggcgttc
     2641 ctgataggcc tgtcggccga gctgctgccg tgggggctgg gcttcgcagc cggggcgatg
     2701 ctgttcgtga tcagccacga ggtgatcccg gaatcgcacc gcaaggggca tgaatcgttc
     2761 gcgaccggcg ggctgatgat cgggttcgtg ctgatgatgg cgctggatac ggtgctggct
     2821 tagaagccct gacaaaaagc cgccagcagc gtcgcgccgc cttgccgtac tgcacgacct
     2881 gcctgcgtcg gcgcgccttg ccatcggcat gttgtcaggg cttcttaggc gcaggggcag
     2941 gtcactgccg cggcaatgcc acctcgaagc tcgtcgtccc ctcccacggc gatgaatgca
     3001 ccgataccgt cccgccgtgg gcgacgacga attcatggac gatgtacagg ccgagtccga
     3061 ggccggccga cggcgtgcgc ttgcggtcgg ggaagtagaa gggcttgaat atcccgtcca
     3121 gcgtctccgg cgggattacg ccttcgttgg tcaggcgcag gcggatggtg ccggcgtccg
     3181 tgccgtcgag ctcgatccgg atcggcgtgc cgtccctgcc gtgctgcagc gcgttgccga
     3241 gcaggttcga gatgacctgc gacatgcggt cggtatcaaa caggccatcg agcttgccgc
     3301 tgctttcgat gcggatgcgc gaggcgctcg cgcgatgctc gatctcggcg atgatgcgcc
     3361 ggcagacgtc ctcgagattg gccttcgatt tgaacagcgt gaccccggcc gcgcgggtct
     3421 gcgcgacgtc cagcagctgg cgcaccatct tttccatgcg cgtgccgctg gcgtgtatgc
     3481 gctctgcggt ctcttcgacg cgcgcgtcgc cgccgatgtg cttgagcagt tccgcgccgt
     3541 gcaggaccgc cgacagcggc gtgcgcaggt catgtccgag gaccgcgatg aacagctcgt
     3601 tcatctgcag cgcccggcgc agctcttcca cctgctcgcg gatctggttt ttctgcgcct
     3661 gcagttcgac gaatacttcc accttgccgc gcagtacatc cgggttgacc ggcttggtca
     3721 ggaagtcgac ggcgccggcc tggtagccgc ggaagctgcg ggtggcttcc tcggccgccg
     3781 cggtcatgaa gatgatcggg atcgccgagg tccgctggtt gccgcgcatc atttcggcca
     3841 gttcgaatcc gtccatgccc ggcatctgca catcgaccag cgccagcgcc acttcatgct
     3901 cgagcagcag ttcaagcgcc tcgaagccgg aggcggcctt gagcaccttg aggcccgggc
     3961 gggcaaggat ggcctcattg gcgacgaggt tctgcgggaa gtcgtctacg acaaggatat
     4021 ggat
//
LOCUS       DATLGF010000402         4668 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_152611, whole genome shotgun sequence.
ACCESSION   DATLGF010000402 DATLGF010000000
VERSION     DATLGF010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152611"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VM406_12325"
     CDS             complement(<1..542)
                     /locus_tag="VM406_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVL76794.1"
                     /translation="MDTLKKSRKEEILDAALACFTEHGVEATTIEMIRERANASVGSM
                     YHHFGGKDRIAAALYIAALAEHHAAQKQLLDAAGDAESGVKAIVYAYVDWISANPDKA
                     RFLLYNRSAVIQGEAVEELRAQARAKRDEVLRVFAPWIEQGTMQRLPKEVFASLIVGP
                     AHDYARLWLSGRAKTDIKAYR"
     gene            710..1633
                     /locus_tag="VM406_12330"
     CDS             710..1633
                     /locus_tag="VM406_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVL76795.1"
                     /translation="MELTTISTEDDFPLAARRFAPGGRPRAVVLLPAAMGVRQDFYFP
                     FARFLAEHGFAALTFDYRGMGASVAPGQSGTLRGFGADLNDWAADYGAALRHARAWHA
                     GVPLLVVGHSLGGQLPGIVPENELIDGIVTVAAGSGYWRDNALPLRRFVWLLWYAIAP
                     LSIRICGYFPGKRLRMVGDLPAGVMRQWGRWCKSPHYISDEAGRPLRAGFDRIRAPVL
                     SLSFTDDEMMSRRSIDSLHDCYRHAQVERRAIAPEEIGARRIGHFGFFREQFRPTLWQ
                     QALRWLEQRALDAARQRQGLDQPARRQLHVV"
     gene            1668..2147
                     /locus_tag="VM406_12335"
     CDS             1668..2147
                     /locus_tag="VM406_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HVL76796.1"
                     /translation="MNLILRLLYVLLISPFRPRLPAGATESRLVLRTLPNDLDINLHM
                     NNGRYLTICDLNRVDMFIRSGIAKLMIKRGWMPIIAEHTMNYKRPLKLFERFEVKMAL
                     THWDAKYFYMTHTFTSGDRIVAEGTSKAVLRSREGVLVPQDVMAALEAQQSGVARPG"
     gene            2335..3441
                     /locus_tag="VM406_12340"
     CDS             2335..3441
                     /locus_tag="VM406_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HVL76797.1"
                     /translation="MKTRAAIAWKAGEPLAVDELDLEGPRAGEVLVEVKATGICHTDY
                     YTLSGADPEGIFPAVLGHEGAGIVVDVGPDVKSLRSGDHVIPLYTPECRQCKFCLSRK
                     TNLCQAIRATQGRGLMPDSTSRFSYEGKPIFHYMGTSTFSNYIVVPEIAVAKIRADAP
                     FDKVCYIGCGVTTGVGAVLFTAKVEAGANVVVFGLGGIGLNVIQAAKMVGADRIIGVD
                     INPAREGMARKFGMTDFVNPNEVDNVVDRIIGLTDGGADYSFECVGNTTLMRQALECC
                     HKGWGQSVIIGVAEAGAEIATRPFQLVTGRVWKGSAFGGARGRTDVPKIVDWYMEGKI
                     NIDDLITHTLSLDQINEGFDLMKRGESIRSVVMY"
     gene            3441..4307
                     /gene="fghA"
                     /locus_tag="VM406_12345"
     CDS             3441..4307
                     /gene="fghA"
                     /locus_tag="VM406_12345"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HVL76798.1"
                     /translation="MTAALEIVSRHRCFDGEQRFYRHNSREIGLPMRFSVYLPPQALD
                     GQACPAVFYLAGLTCTEETFMIKAGAQRVAAREGMILVAPDTSPRGANLPGESDNWDF
                     GVGAGFYLDATVEPWSRHYRMRSYVAVELMGLLVDGHLGMDVHRVGIMGHSMGGHGAL
                     VLALHHPQLFRSVSAFAPIAAPSQCPWGQKAFRGYLGDDEVQWAQYDATALMLAHRHA
                     PYPHGILIDQGLDDKFLDEQLYPDLFEDACRQAGQPLTLRRHPGYDHGYYFISSFVED
                     HLLHHKAQLVRG"
     gene            complement(4469..>4668)
                     /locus_tag="VM406_12350"
     CDS             complement(4469..>4668)
                     /locus_tag="VM406_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833356.1"
                     /note="molecular chaperone; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HVL76799.1"
                     /translation="PESKPILEINPNHPLVQRLKYEEAKFDDWSHILFDQASLAEGGT
                     LADPAGFVKRLNEMLLGMAGK"
BASE COUNT          781 a         1511 c         1538 g          838 t
ORIGIN      
        1 cggtaggcct tgatgtcggt cttcgccctc ccggacagcc acagccgggc atagtcgtgg
       61 gcggggccga cgatcagcga cgcgaacact tccttcggca gccgctgcat cgtcccctgc
      121 tcgatccacg gcgcaaacac ccgcagcacc tcgtcgcgct tggccctggc ctgcgcgcgc
      181 agttcctcca ccgcctcgcc ctggatcacc gcgctgcggt tgtagagcag gaagcgcgcc
      241 ttgtccgggt ttgccgaaat ccagtccacg tacgcataga cgatcgcctt gacgcccgac
      301 tccgcgtcgc ctgccgcatc cagcaactgt ttctgcgccg catgatgctc ggcaagcgcc
      361 gcgatgtaga gcgcggcggc gatgcggtcc ttgccgccga agtggtggta catgctgccg
      421 acgctggcgt tggcccgctc gcggatcatc tcgatcgtgg tcgcctccac gccgtgctcg
      481 gtgaagcagg cgagggcggc atccaggatt tcttccttgc ggctcttctt tagagtgtcc
      541 attcgatttc ggtcatggtt agaacatcat tctagaataa tgttctagaa tgtgattcta
      601 gtaacggcgg cgacagagcg aaccttaccg cagcgccgtt cccagggcaa acgatgcgca
      661 ggcgcataca tgcagggccg caagccgcgc taacaccgat ggaggcaaga tggaactcac
      721 caccatttcc accgaggacg attttccgct cgcggcgcgt cgctttgctc cgggcggcag
      781 gccgcgcgcg gtggtgctgc tgccggcggc gatgggcgtg cggcaggact tctattttcc
      841 attcgcgcgc tttctcgccg agcatggctt cgccgcgctg accttcgact accgcggcat
      901 gggcgcttcg gtggcgccag gacaaagcgg cacgttgcgc ggcttcggtg ctgacctgaa
      961 cgactgggcc gccgactacg gcgccgcgct gcgccatgcg cgcgcctggc acgccggcgt
     1021 gccgctgctg gtcgtcgggc actcgctggg cgggcagttg ccaggcatcg tcccggagaa
     1081 cgagctgatc gacggcatcg tcacggtggc cgccggcagc ggctattggc gcgacaacgc
     1141 cctcccgctg cggcgtttcg tctggctgct gtggtacgcg atcgcgccgc tttccattcg
     1201 catctgcggc tactttcccg gcaagcgcct gcgcatggtc ggcgacctgc cggcaggcgt
     1261 gatgcgccag tgggggcgct ggtgcaagag cccgcactac atctccgatg aagctggccg
     1321 cccgctgcgg gccggcttcg accgcatccg cgcgccggtg ctcagcctga gcttcaccga
     1381 cgacgaaatg atgagccggc gcagcatcga cagcctgcac gactgttacc ggcatgcgca
     1441 ggtcgaacgc cgcgcgattg cgccggaaga gatcggcgcg cgccgcatcg ggcacttcgg
     1501 cttttttcgc gaacagtttc gccccacgtt gtggcagcag gcgctgcgct ggctggagca
     1561 gcgcgcgctg gacgcggcgc ggcagcggca ggggctcgat cagccggcac gcaggcaact
     1621 ccacgtcgtc tgaccctgta tctcgccact ttctgactgg agcccccatg aatttgatcc
     1681 tgcgtcttct gtatgtcctg ctgatctcgc cgttccgtcc gcgcctgccg gccggtgcaa
     1741 ccgagagccg gctggtgctg cgcaccttgc ccaacgacct cgacatcaac ctgcacatga
     1801 acaacggccg ctatctgacg atctgcgacc tgaaccgggt cgacatgttc atccggtccg
     1861 gcatcgcaaa gctgatgatc aagcgcggct ggatgccgat cattgccgaa cacacgatga
     1921 actacaaacg gccgctgaag ctgttcgagc gcttcgaagt gaagatggcg ctgacgcact
     1981 gggatgcgaa gtacttctac atgacgcaca ccttcaccag cggcgaccgc atcgtcgccg
     2041 agggcacgtc caaggcggtg ctgcgcagcc gggagggcgt gctggtgccg caggacgtga
     2101 tggcggcgct cgaagcgcag cagagcgggg ttgcccggcc cggatgatcg tctgcgccag
     2161 agcggcgttg cgccgaatca tgtgttccga acgttgacgg cgggacaggc gtggtaagaa
     2221 cctccgtacg gatgcgcctg tcttagcgtg acccgcaacc cgcgaggcgc gcggccgcct
     2281 tccatgcaag cgtgctagcg cggctgcgtc tcgtgttctt tccggaaggc aggaatgaaa
     2341 acaagagccg caatcgcatg gaaggccggc gagccgctgg ccgtcgatga actcgatctc
     2401 gaaggcccgc gcgccggcga ggtgctggtc gaggtcaagg ccaccggcat ctgccatacc
     2461 gactactaca cgctgtccgg tgcggatccc gaaggcatct tcccggcggt gctcgggcac
     2521 gaaggggcgg gcatcgtggt ggatgtgggc ccggacgtga aaagcctgcg cagcggcgat
     2581 catgtgattc cgctgtacac gcccgaatgc cggcagtgca agttctgcct gtcgcgcaag
     2641 accaatctgt gccaggcgat ccgcgcaacc cagggcaggg gactgatgcc ggactccacc
     2701 tcgcgcttct cgtatgaagg caagcccatc ttccactaca tgggcacctc gacgttctcc
     2761 aactacattg tcgtgcccga aatcgcggtg gcgaagatcc gcgcggatgc gccgttcgac
     2821 aaggtctgct atatcggctg cggcgtcacc accggcgtgg gcgcggtgct gtttacggcg
     2881 aaagtcgagg ccggcgcgaa cgtggtcgtg ttcggtctgg gcggcatcgg cctgaatgtg
     2941 atccaggccg cgaagatggt cggggcggac aggatcatcg gcgtcgacat caatcccgcg
     3001 cgcgaaggca tggcgcgcaa gttcgggatg acggatttcg tgaatccgaa tgaagtcgat
     3061 aacgtcgtgg atcgcatcat tggcctgacc gacggcggcg ccgactattc attcgaatgt
     3121 gtcggcaaca cgacgctgat gcgccaggcg ctcgaatgct gccacaaggg atgggggcag
     3181 tcggtgatca tcggcgtggc cgaggcaggg gccgagatcg ccacgcgccc attccagctg
     3241 gtcaccggcc gggtctggaa gggctcggcc ttcggcggcg cacgcgggcg caccgatgtg
     3301 ccgaagatcg tcgactggta catggaaggc aagatcaata tcgacgacct gatcacccac
     3361 acgctgtcgc tcgaccagat caacgaaggc ttcgacctga tgaagcgcgg cgaatcgatc
     3421 cgttcggtgg tgatgtactg atgactgcgg cactcgaaat cgtatcccgg caccgctgct
     3481 tcgacggcga gcagcgcttc taccgtcaca actcgcgcga gatcgggctg ccgatgcgct
     3541 tctcggtcta tctgccgccg caggcgctgg acgggcaggc ctgccccgcg gtgttctacc
     3601 tggccggcct gacgtgcacc gaagaaacct tcatgatcaa ggccggcgcg cagcgagtcg
     3661 cggcgcgcga gggaatgatc ctcgtcgctc ccgacaccag tccgcgcggc gccaacctcc
     3721 ccggcgagag cgacaactgg gatttcggcg tcggggccgg cttctacctc gatgcgaccg
     3781 tcgagccctg gtcgcgccac taccggatgc gcagctacgt cgcggtggag ctgatgggac
     3841 tgctggtcga cggccacctg ggcatggacg tgcatcgcgt gggcatcatg ggccattcta
     3901 tgggcggcca cggcgcgctg gtgctcgcgc tgcatcaccc gcagctgttc cgctccgtgt
     3961 cggcgttcgc gccgattgcg gcgccgtcgc aatgtccgtg ggggcagaaa gcctttcgcg
     4021 gctatctcgg ggatgacgag gtgcagtggg cgcagtacga cgcgaccgcg ctgatgctcg
     4081 cgcatcgcca cgcgccgtat ccgcacggca tcctgatcga ccaggggctg gacgacaagt
     4141 tcctcgatga gcagctctat ccggacctgt tcgaggacgc ctgccgccag gcagggcagc
     4201 cgctgacgct gcgccggcat ccgggctacg accacggcta ctacttcata tccagcttcg
     4261 tcgaggatca tctgctgcac cacaaggcgc agctggtgcg ggggtaggga gggggtgtga
     4321 acctcgcact gacgggatga ggttcctcgc ttcgctcgga acgatggcat tggggccgtc
     4381 gcccgattac tgtcgtccgg aacgcaggga gggatctcag ccgcctgcca agcgaggctc
     4441 gcagcgactg gacgaggtca acgagttctt acttccccgc catccccagc agcatctcgt
     4501 tcaagcgctt cacgaagccc gccggatcgg ccagcgtgcc gccctcggcc agcgaggcct
     4561 gatcgaacag gatgtgcgac cagtcgtcga acttcgcctc ttcgtatttc aggcgctgca
     4621 ccagcgggtg gttcgggttg atctcgagga tgggcttgga ttccggcg
//
LOCUS       DATLGF010000403        12627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_62756,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000403 DATLGF010000000
VERSION     DATLGF010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 12627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12627
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62756"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VM406_12355"
     CDS             <1..632
                     /locus_tag="VM406_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76800.1"
                     /translation="AGQEAFRFKVLLNGEPWLEASAEAGAGEVALPPFEPGNHRLTVV
                     SDMKARWYASHQEAGDPWVRRRAWRFDTPLQFEVERLTSEAEFIAVRVFRPAGSARQS
                     ARTNLRVRIQAPKQQEAFAPMPGWLFTERAYDVRASGEHALAVAETSGERSDAGQPLY
                     IAIPKGAPAGRYRITLEPEDGQGWIAVSRVTPGAQRTARLVLEEAGDAS"
     gene            793..1503
                     /locus_tag="VM406_12360"
     CDS             793..1503
                     /locus_tag="VM406_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76801.1"
                     /translation="MEKLFVRLLQGERTAQIDTALRALGWAMRTQADGAVTWTVVAEA
                     DTRREGRGLFAFSSHGRHAIQAPHVPTDALTGQILLGYGRDALPRALAWNTVPRRTAD
                     MARLDDTPLVAFSRAFAQVHPQEKILQLHGFDVANRRSGAGATSSAIVSAGHRRPSPA
                     LVSAVTCMQKRIDQRARLYGNDVHELGGTQNSVARTLLASGHAGFIHVELDMPLRRSL
                     VDDAARRRVLLECLGGRS"
     gene            1503..6515
                     /locus_tag="VM406_12365"
     CDS             1503..6515
                     /locus_tag="VM406_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76802.1"
                     /translation="MQRLITCMAAAGLIAAYPAWAAIDGLDAARVGAAISLHWENIKG
                     APYLVSGSRARASTWRGLHSVELEGGKDVVVHLPAHALLRVMLEDGPAHAPLFYVSDG
                     TGLAVQQQPVAGGDGKSWLLRTDSTRPVLVHIRGAAVSYAPQRVALLLARYEAPQHAL
                     GYRHEMTLAGTRVKLRAADESTARNYVRLAADRELEIPVRGPDRVVLEYRADAAPVSD
                     GILNMEVRLDGELLRQVRDLIGPDHVTPVKIAGAWTRAGARRRIAFDVPAGDHTLHVR
                     SSHGVLLRAARTGGSGFLLPQLNMPAAWQDQAAESGLEALEQESIAAAMSNQWRDIGV
                     LAAGKLREEARARPDMASLAKAQREMEQAFTRFRDLLPDGPNDVDVRSVVLQTARTPG
                     EAAVNRVLSPEIDPLPQARFHRIGADPVRFTLPAVAHPLRLRALLPREANDVRVEVTD
                     ERGKVLLLRADVHQVGADALRIPALSPAQNEGWPLSLDGLADRLDAAAPAIQAASAEW
                     VVPAGVRSLSLRALDGAASLALQWEASARYEGDDAMLAAMVRQRSTQGTGAGAFKAAS
                     LPLQRMLDAAHASFTANVVPMRSGHSPVQPVQARAALEAASGVKDPVHALRLWQQALQ
                     ADEAELRARALRGKVATLLDAGERFAAERMLRAWWIGDDAALASGAADELDALYRREG
                     DRDMQLQFAAARAAREPARLAQLSALLSADGQDARALLAGLASPDYQSRAMLQSALRS
                     GNWQTFDALLEQVREPADAAFWRAQRALSLGRHDEAARLFAEANAADWGRALAQGRVI
                     SAQLFDAEADKAAAVQRWLQWQAAHPGARVWRDEAGSVLRHAGGVNLRNKTLDTSAPW
                     WRANAGQALVAQVVGPAKIRVEARPVHRTAEMRLDGWLRVKAADQLWVQAFNQNQTAP
                     DLEIGAGTDMPGTAVVREIALPAGVHELHIDAGDTEVIARLQIERPALQIPALPAPAA
                     AHFKPGSFAHARQLPAAACVINAGCVLVADRGLSANRLDIENVDWTGLPAPAMERDPV
                     AASLAAGNTKAALEQSKDPLERMRLLRWLAEEQPAQRTRALAEAAQLAKAHNDREIRS
                     HWNQLQAAGRWSLLPVVDRSAGLRRLEVARGVPESPAARLRAVMLPALQEGEIRLGAA
                     SQANLLLNNDRAGSLVLEATVDELPGEPPLPVQLRITRNGQEVHAAELKQSNGTVRIP
                     LSVPAGEQQISVSLRQAYANQSLRLRFTGAAQPEARVVREWHIATAEQPVQATLAGPV
                     WVRIDRIEGDAVHSEERLMTESMSRLVLTPHAGSKESLYRIHQLQADPAPSQAAPVRP
                     NAYTPTMMPDAPSSWSSAPADAAPGQVRFIDSETLASDSGGTLSLRASMQSRRNSDAG
                     GGRSDPPLERYTETGASWRTANQTETRHLLADVFARKREIGDPVLGFRLNAHQEFGWT
                     PLLAMPFAADVSLRGFTQNTPHGRGTALAASAEVYQERALSATLSHRPAVEMNWYSMN
                     LTGVANPLRVDSDVFTTYRSRHLRALTLSERLSWRPWRDTHISARISATTNPDLDLRR
                     IDHHRLELQWRQLAGPVMLEAGGRLTRYWEDIDRPGNITRREVHVGATGDWWVARGNR
                     IEVRGQVRRDLDTSATWGGVELRWHWGPGRQLRDFSPAELDFGAVRGWRMPGNGNRME
                     EE"
     gene            6512..10777
                     /locus_tag="VM406_12370"
     CDS             6512..10777
                     /locus_tag="VM406_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76803.1"
                     /translation="MKSTEQGLADLLTQGLRPYELKLEQQQVAAIADGWAAWRARQDA
                     RASDTSELLAVRYLQERLATGLREISDLREGQARFTRRCAQVGSPEELRRVILDYALE
                     LGASRRQLRGDARALRRWFDADAMHDRVRVRIANLERSTIFCLKSLGRMAARVLRHMQ
                     DQKMAWELMRLEQGLYPLLEWDGDPRVRLEAFRALSAALRELLPGLALHAVGKELAAF
                     IVRSSLDKRLDVWIQREALLLLSCCDVQRFADTVERRLRDAGDGDDLFVRRFAVERLG
                     VVGISTERRDLLIGIAVHDSSPYVRQAVAQALACLTPSVAQRYWPVLGIEDPTPQVRA
                     AAILQIPRLATQAAHFEAMLHCLGIVLRKERTSFCLRVALRSVEEGALSLEGARQQLW
                     MKTLLPLVEELHVECPVLAVRRWAAEARERIVCQAGSESRALAREIAVLTRECEPGNA
                     ADMAVPVEDVDSLGRVLACTVQRDHGLDLERRGGAWRAWRGHRFGFRMWRFLHEWKHP
                     SPDKRQAFRHTVGRVFHGTVRAPSGILAELAQTKVPGEPLHMSSEAGWRPYLPLVDEL
                     ISLLDESPSSGPLRLYTAEGRTEIAAPSSLTKRLAMRMKLTRDFEKYARARNWNEGSS
                     ADPGSYLKMIGELGFEVRYQPYPGTRADPAVTRFFPGLPAFAAFPLPEMHNRLENYFY
                     SAYDNSLFDLAVFTGVAMAGFLGHHVWVNLRMRAARRALPLVIGGWGTRGKSGTERLK
                     AGLFNALGYGVVSKTTGCEAMFLYAPPQGELREMFLFRPYDKATIWEQYNVVRLANRL
                     GCEVFLWECMALTPAFVRLLQRSWMRDDYSTITNTFPDHEDLQGPAGINIPQVMTEFI
                     PEKRVVLTAEEQMRPILQSAADALGTELKGVGWLESGLIAPDILARFPYQEHPDNIAL
                     VAALGTELGIDYDFSLKAMADKVVPDLGVLKTFPVARVGSRRLEFSNGMSANERYGCL
                     GNWERLGLARQDPFAEPGVCISTVVNNRADRVARSRVFAGVLTQDIRADFHVLIGSNL
                     DGLRGYIKEAWHAHAQGLSLKRAHETEETPVRALERIAREYRVVHADAHVSDRLAVML
                     KGIGAPEQLVEHAGDVEALGAALAQQGIGNGGALLDFHARDLRTLSEYRALETRCQRA
                     GETAADLDAALREQMGIWFERRIVVVEDYYASGDAVIATIRDATPPGFVNRIIGLQNI
                     KGTGLDFVYRWLAWESCHKACQDLLSADPAQQRRGLASLARFQEFGLLCETETEAALA
                     ALQATPQGQKQHVRAEIKTIAARMTERMAQIDSSASSARVQGWKEKVINFVEGFLDAG
                     DAVRRRRRSDRIYDDLVNRRISEARAALELQALTRRQKGGWLGEAISAWGKRLSERLR
                     IRDSAARRELGRSSRVPVPESGSDVSGAQAASRAQARPERVES"
     gene            complement(10931..11056)
                     /locus_tag="VM406_12375"
     CDS             complement(10931..11056)
                     /locus_tag="VM406_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76804.1"
                     /translation="MKVEYQSCKNEKTAISGGFVRNFGLLICSRAYRSSTAKGLE"
     gene            11069..12133
                     /gene="aroG"
                     /locus_tag="VM406_12380"
     CDS             11069..12133
                     /gene="aroG"
                     /locus_tag="VM406_12380"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HVL76805.1"
                     /translation="MPRTDDLRIREMKELIPPSHLIREFSVSEKAANVAANARVALHR
                     ILHGQDDRLMVVIGPCSIHDPKAALEYARRLVEQRDRFSADLEIIMRVYFEKPRTTVG
                     WKGLINDPYMDNSFRINDGLRMARELLLNINELGLPAGTEFLDVISPQYVADLISWGA
                     IGARTTESQVHRELASGLSCPVGFKNGTDGNIKIAVDAIKAASQPHHFLSVTKGGHSA
                     IVATAGNEDCHIILRGGKTPNYDAASVDAACRDIAANGLASRLMIDASHANSSKKPEN
                     QVPVCKDIGAQVAGGDTRIVGVMVESHLVAGRQDLVPGRELTYGQSITDGCIGWEESV
                     KVLEVLAESVRKRRLHGDDE"
     gene            complement(12324..>12627)
                     /locus_tag="VM406_12385"
     CDS             complement(12324..>12627)
                     /locus_tag="VM406_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HVL76806.1"
                     /translation="GSVQMLLDTGATLIALPAADARRLGIDYRNGAIRYLNTANGVVP
                     AYLVRLDRVKVGDIELHGVDAVVQEQGLPIILLGMSFLNRTEMRREGTQMVLTRRF"
BASE COUNT         2126 a         4082 c         4325 g         2094 t
ORIGIN      
        1 gtgccgggca ggaagccttc cgcttcaagg tcttgctgaa cggcgagccc tggctggagg
       61 cctcggccga ggcgggcgcc ggtgaagtgg cgctgccgcc gttcgaaccc ggcaatcacc
      121 ggctcaccgt ggtcagcgac atgaaggcgc gctggtacgc gagccaccag gaagccggcg
      181 acccctgggt gcgcaggcgt gcctggcgct tcgatacgcc gttgcagttc gaggtcgagc
      241 gattgaccag cgaagccgag ttcatcgcgg tacgcgtgtt ccggccggcg ggcagtgccc
      301 gccagagcgc gcggaccaat ctgcgcgtac ggatccaggc cccgaagcag caggaggcgt
      361 tcgcccccat gcccggctgg ctgttcaccg agcgtgcgta tgacgtgcgc gccagcggcg
      421 agcatgcgtt ggctgtcgcg gaaacgagcg gcgaacgcag cgatgccgga caaccgctct
      481 acatcgcgat tccgaagggc gcgccggccg gccgctaccg gatcacgctc gagcccgagg
      541 acgggcaggg ctggatcgcg gtgtcgcgcg tgacgccggg cgcgcagcgc acggcacggc
      601 tggttcttga agaggcaggg gatgcaagtt agcagggcgc ttgttttgct cgtatcagcc
      661 gggttctccg cagccgtgag tgcaaagacc gccgtagcgc cggacgggct gctggagcag
      721 gcctatgcac gggcctggaa aggtgccgat tcggtgcaat acgtcccgtt gcgacgcggc
      781 gagcgtgaag cattggagaa gctgttcgtg cgcctgttgc agggcgaacg gacggcgcag
      841 atcgacacgg cactgcgcgc cctcggctgg gccatgcgca cgcaagctga cggtgcagtc
      901 acatggaccg tcgtcgccga ggccgacacg cggcgcgagg ggcgcgggct gtttgccttt
      961 tccagccacg gcaggcatgc gatccaggcg ccccatgtgc ccaccgatgc cctgaccgga
     1021 cagatcctgc ttggctacgg ccgcgatgcg ctgccgcgcg cgctggcctg gaacacggtg
     1081 ccgcggcgca ccgccgacat ggcgcgactg gacgacacac cgctggtcgc attttcgcga
     1141 gcgttcgcgc aagtccatcc acaggaaaag attctgcaac tccacgggtt cgatgtggcc
     1201 aaccggcgct cgggcgctgg tgcgacaagc agcgccatcg tcagtgccgg ccaccgccgg
     1261 ccttcacccg ctttagtttc tgctgttaca tgtatgcaaa agagaattga tcaacgcgcg
     1321 cgtctgtatg gcaacgacgt gcacgaattg ggcggcacgc agaatagtgt cgcccgtacg
     1381 ctgctggcga gcggccatgc cggcttcatc catgtcgaac tggacatgcc gctgcgccgc
     1441 agtctggtcg atgatgcggc ccggcgccgc gtgctgctgg aatgcctcgg aggcaggtcc
     1501 tgatgcagcg cctgatcacc tgcatggcgg ctgccggact cattgccgcc tatcccgcct
     1561 gggccgccat cgacggtctc gacgccgcgc gtgtcggcgc ggccatcagt ctgcactggg
     1621 aaaacatcaa gggcgcgccc tacctggttt ccggttcgcg ggccagggcc tccacatggc
     1681 gcggcctgca cagcgtggaa ctggagggcg ggaaggacgt ggtcgtgcac ctgccggcgc
     1741 atgcgctgct gcgcgtgatg ctggaagacg ggccggcgca tgcgccgttg ttctacgtgt
     1801 ccgacggcac tggcctggcg gtgcagcagc agccagttgc aggtggcgac ggcaagtcct
     1861 ggctgctgcg caccgattcg acccgtccgg tgctggtaca catccgcggc gccgccgtct
     1921 cctatgcgcc gcagcgggtg gcccttctac tcgctcgcta cgaggcgccg cagcacgcgc
     1981 ttggctatcg tcatgagatg acgctggcag gcacgcgcgt gaaactgcgg gcggccgacg
     2041 aatcgaccgc gcgcaactac gtccggctgg cagcagatcg cgagctggag atcccggtgc
     2101 gcgggccgga ccgggtcgtg ctggaatacc gcgcagatgc agcgccggtt tccgacggca
     2161 tcctgaacat ggaagtgagg ctggacgggg agctgctgcg acaggtccgc gacctgatcg
     2221 gcccggatca tgtgacgccg gtgaagatcg ccggcgcgtg gacgcgcgcc ggtgcccgca
     2281 ggcgcatcgc cttcgacgtg ccggccggag accatacgct gcatgtgcgc agcagccacg
     2341 gcgttctgct gcgtgcagca cgcaccggcg gaagcggctt tctcctgccg cagctcaaca
     2401 tgccggccgc atggcaggac caggccgctg agtccgggct ggaggcgctc gaacaggaga
     2461 gcatcgcagc tgcgatgtcg aatcagtggc gcgacatcgg cgtcctcgct gcaggcaagc
     2521 tgcgtgagga agcgcgggcc cgtcccgaca tggcgagtct ggcgaaggcg cagcgcgaaa
     2581 tggagcaggc gtttacccga ttccgcgacc tgcttcccga cggccccaac gacgtcgatg
     2641 tgcgcagcgt cgtcctgcaa accgcacgca cgccgggcga ggcagcggtc aaccgtgtgc
     2701 taagcccgga gatcgatccg ttgccgcagg cgcgcttcca tcgcatcggt gccgatccgg
     2761 tacgtttcac acttcctgcc gtcgcgcatc cgctgcgcct gcgtgcgctg ctgccgcgtg
     2821 aggcaaatga tgtacgcgtg gaggtcacgg acgaacgcgg caaggtactc ctgctcaggg
     2881 cagacgtaca ccaggtcggt gcggatgcat tgcggattcc cgcgctgagt ccggcgcaga
     2941 acgagggctg gccgctgtcg ctggacggtc tcgccgaccg gctcgatgcg gcggcacccg
     3001 cgattcaggc ggcaagcgcg gagtgggtag tcccggccgg tgtgcgcagc ctgtcgttgc
     3061 gtgctctcga cggcgcggcc tcgctcgctc tccaatggga agccagtgcc cgctacgagg
     3121 gcgacgacgc catgctcgca gccatggttc gacagcgctc gacgcaggga actggcgcgg
     3181 gagcattcaa ggccgcatcc ttgccgctgc agcgcatgct cgatgcggct catgcaagct
     3241 tcaccgccaa cgtggttccg atgcgcagcg ggcattcgcc cgtgcagccg gtccaggcca
     3301 gggcggcgct ggaagccgca agcggggtga aggatccggt ccatgcattg cgcctgtggc
     3361 agcaggcttt gcaggccgat gaggccgaac tgcgcgcacg cgcattgcgc ggcaaggtcg
     3421 cgacgctgct ggatgcgggc gagcgctttg ccgccgagcg catgctgcgg gcctggtgga
     3481 tcggtgacga cgccgcgctg gcaagcgggg cagccgatga actcgacgcc ctgtaccggc
     3541 gcgaaggcga tcgcgacatg cagctgcagt tcgccgccgc gcgggcagca cgcgagccgg
     3601 cgcgcctggc gcagctcagt gcgctgctga gcgcagatgg ccaggatgcg cgggcactgc
     3661 ttgcgggact cgcatccccg gattatcagt cgcgtgcaat gctgcagagc gcgctgcgtt
     3721 ccggaaactg gcagaccttc gacgccttgc tggaacaggt ccgggagcct gctgatgcgg
     3781 cgttctggcg tgcccagcgc gcgctgtcgc tcggccggca tgacgaggcc gcgcgcctgt
     3841 tcgccgaggc caatgccgca gactggggca gggcgcttgc ccaagggcgg gtgatttccg
     3901 cgcaactctt cgacgccgaa gcggacaagg cagcagcggt gcagcgctgg ctgcagtggc
     3961 aggccgcgca tcccggcgcg cgggtatggc gcgacgaagc cgggtcagtg ctgcgccatg
     4021 cgggcggtgt caacctgcgc aacaagacgc tcgatacgtc cgcgccgtgg tggcgcgcaa
     4081 atgccgggca agcgctggtc gcgcaggtcg tcggccccgc gaaaatccgc gtcgaggcaa
     4141 ggcccgtgca ccgcacggcc gaaatgcgtc tggacggctg gctgcgcgtg aaggccgcgg
     4201 accagctctg ggtgcaagcc ttcaaccaga atcagactgc acccgatctc gaaatcggcg
     4261 ccggcacgga catgcctggt acggccgtcg ttcgcgagat tgcactgcct gccggtgtgc
     4321 acgaactgca tatcgatgcc ggcgataccg aggtgattgc gcgcctgcag atcgaacgcc
     4381 cggcgctgca gattcctgca ttgccggcgc ccgctgccgc tcatttcaag cccggctcct
     4441 tcgcacatgc gcggcagctc cctgccgccg catgcgtgat caacgcgggt tgcgtactgg
     4501 tcgccgatcg cggcctgagt gccaacaggc tggacatcga aaacgtggac tggaccggct
     4561 tgccggctcc ggccatggag cgcgatccgg tggccgcatc gctcgctgcc ggcaatacga
     4621 aagcggcgct tgaacaaagc aaggacccat tggagcgcat gcgcctgctg cgctggctgg
     4681 ccgaggagca gcctgcccaa cgcacgcgcg cgctggccga agccgcacaa ttggcgaagg
     4741 cacacaatga ccgcgaaatc cgttcgcact ggaatcagct gcaggcggcg gggcgctgga
     4801 gcctgctgcc cgtggttgac cgcagcgccg ggctgcgccg gctcgaggtg gcgcgcgggg
     4861 tgccggaatc gcccgctgcc cgcctgcgcg cggtcatgct gccggcgttg caggaaggcg
     4921 agatccgtct cggggcggct tcccaggcga acctgctgct gaacaacgac cgtgccggct
     4981 cgctggtgct tgaagccact gtggacgagc tgcctggcga gccaccattg cccgtgcagt
     5041 tgcgcatcac gcgcaacggg caggaggtgc acgcggccga attgaagcag tcgaacggga
     5101 ccgtgcggat tccgctctcg gtgcctgccg gcgaacagca gatatcggtc tccctcaggc
     5161 aggcttatgc gaatcaatcc ttgcgcctgc gcttcaccgg tgccgcgcag ccggaagcgc
     5221 gcgtcgtgcg tgaatggcat atcgcaacgg cggaacagcc cgtgcaggcg acgctggccg
     5281 gcccggtctg ggttcgcatc gatcgcatcg agggcgatgc agtgcacagc gaagagcggc
     5341 tgatgacgga atcgatgtcg cggctggtgc tgacgccgca tgcgggcagc aaggagagcc
     5401 tgtatcggat ccaccagctg caggccgatc ctgcgccctc gcaagctgca ccggttcggc
     5461 cgaacgccta cacgccgacg atgatgcccg atgccccgtc aagctggagc agcgctccgg
     5521 cggacgctgc tccgggacag gtgcgcttca tcgattcaga aacgctcgcc agcgattccg
     5581 gcggaaccct gagcctgcgc gcaagcatgc agtcgcgccg caattccgat gcaggcggag
     5641 gccggtccga tccgccgctg gagcgctaca cggaaaccgg cgcgagctgg cgcacggcga
     5701 accagaccga aacccgccat ctccttgccg atgttttcgc gcgcaagcgc gagatcggcg
     5761 acccggtgct gggattcagg ctcaacgcgc accaggagtt tgggtggacc ccgctgctgg
     5821 cgatgccgtt tgcagccgat gtctcgctgc gcggctttac gcagaacacc ccgcacggca
     5881 ggggcactgc gctggcagcc tccgccgagg tctatcagga gcgagcgctg agtgcgacct
     5941 tgtcgcaccg gccggccgtg gaaatgaact ggtactcgat gaacctgacc ggcgtggcca
     6001 acccgctgcg cgtcgactcg gatgtgttca ccacttaccg ttcgcgccat cttcgcgcgc
     6061 tcaccttgag cgaacgactg tcctggcgcc cctggcgtga cacccacatc tcggcccgca
     6121 tatcggccac taccaatccc gacctggacc tgcgccgcat cgatcaccac cggcttgaac
     6181 tccagtggcg ccagctcgcc ggcccggtga tgctggaagc aggcggacgg ctgacgcgct
     6241 actgggagga catcgaccgg cccggcaaca tcacccgccg cgaagtccat gtcggcgcca
     6301 ccggagactg gtgggtcgcg cgcggaaacc ggatcgaagt acgggggcag gtgcggcgcg
     6361 atctggacac gtccgccacc tggggcggcg tcgagttgcg ctggcattgg ggccccggcc
     6421 ggcagctgcg ggatttctct cccgctgaac tggactttgg cgcggtgcgc ggctggcgca
     6481 tgccgggcaa cggcaatcgc atggaggaag aatgaaaagc accgagcaag ggctcgcgga
     6541 cttgctgacg cagggattgc gcccgtatga actgaagctg gaacaacagc aggtggcggc
     6601 gatcgccgac ggttgggctg cctggcgggc acgccaggat gcgcgtgcca gcgatacgtc
     6661 cgagctgctg gctgtgcgct acctgcagga acggctggcg accggcttgc gcgagatttc
     6721 ggacttgcgc gaaggccagg cacgctttac ccgccgttgt gcgcaggtcg gttctcccga
     6781 agagctgcga cgtgtgatcc tcgattacgc gcttgaactg ggcgcgtcgc gccgtcagct
     6841 gcgcggcgac gcgcgcgcat tgcgccgctg gttcgatgcc gacgccatgc acgaccgggt
     6901 gcgcgtgcgc atcgcaaacc tggagcgcag cacgatcttc tgcctgaagt ccctgggccg
     6961 gatggccgcg cgcgtcctgc gccatatgca ggatcaaaag atggcatggg agttgatgcg
     7021 gctggaacaa gggctttatc ccttgctgga atgggatggc gatccgcgtg ttcgcctgga
     7081 agcctttcgc gcattgtccg ctgcgttgcg cgaactgctc cccggtctgg ctttgcacgc
     7141 agttggcaaa gagctggcgg cgttcatcgt tcggtcctcg ctcgacaagc gcctggacgt
     7201 gtggatccag cgggaagcgc tgttgctgct gtcatgctgt gacgtgcagc gtttcgcaga
     7261 taccgtcgaa cgacgcctgc gcgacgccgg agacggtgac gatctgttcg tccggcgttt
     7321 tgctgtcgag cggctcggcg tcgtcggcat ttccactgag cgccgcgacc tgctcattgg
     7381 cattgccgtc catgacagca gcccctatgt gcgccaggcg gtggcgcaag cattggcgtg
     7441 tctgacgccc tcggtggcgc agcgctactg gccggtgctc ggcatcgaag acccgacgcc
     7501 gcaggtgcgg gccgctgcaa tcctgcagat cccgcgcctc gcaacacagg ccgcgcactt
     7561 cgaagcgatg ctgcattgcc ttggcatcgt actgcgcaag gaacgcacgt cattctgcct
     7621 gcgggtggcg ctccggtccg tggaagaggg cgcgttgtcg ctggaaggcg caaggcagca
     7681 gctctggatg aaaacgctgt tgccgctggt cgaggaattg cacgtcgaat gtccggtact
     7741 tgccgtgcgg cgctgggccg ccgaggcgcg cgagcgcatc gtctgccagg cgggcagtga
     7801 aagccgggcc ctggcacgcg agatcgcggt gctgacccgg gagtgcgaac cgggcaatgc
     7861 ggccgacatg gcggtgccgg tcgaggacgt cgacagcctc gggcgtgtgc tcgcctgcac
     7921 ggtccagcgc gaccatggat tagatcttga gcggcgcggc ggcgcatggc gtgcatggcg
     7981 cgggcaccgc ttcggttttc gcatgtggcg ctttctgcat gagtggaagc atccgtcgcc
     8041 ggacaagcgc caggcattcc ggcacacggt gggacgggtc tttcacggca cggtgcgcgc
     8101 accgtcaggc atcctcgcgg aactggcgca gaccaaggtg ccgggcgagc ccctgcacat
     8161 gtccagcgaa gcgggctggc gtccctacct gcccctggtg gacgagctga tttccctgct
     8221 cgacgaaagt ccgtccagcg gccccttgcg cctgtacacg gccgaaggac ggaccgagat
     8281 cgcggcacca tcctcgctga ccaagcgcct ggcaatgcgc atgaagctca cgcgcgactt
     8341 cgagaagtat gcgcgcgcgc gcaactggaa cgaaggaagc tccgccgatc ccggcagtta
     8401 cctgaaaatg attggcgagc tcgggttcga ggtgcgctac cagccctatc cggggacacg
     8461 cgccgatcct gcggtgacgc gcttctttcc gggtttgccg gccttcgccg ccttccccct
     8521 gcccgagatg cacaaccggc tggagaatta tttctactcc gcctatgaca acagcctgtt
     8581 cgatcttgca gtcttcaccg gcgtggcgat ggccggtttc ctcggtcacc acgtctgggt
     8641 gaatctccgc atgcgcgccg cgcgacgcgc gcttccgctc gtcatcggcg gctggggcac
     8701 gcgcggaaaa tcgggcaccg agcgattgaa ggcgggcttg ttcaatgcgc tcggctatgg
     8761 cgtcgtcagc aagacgaccg gctgcgaggc catgttcctg tacgcgccgc cgcagggcga
     8821 gctgcgcgaa atgttcctgt tccgtcccta tgacaaggcc acgatctggg agcagtacaa
     8881 cgtggtgcgc cttgccaatc ggctgggttg cgaggtgttt ctctgggaat gcatggcgct
     8941 cacgccggcc ttcgtgcgcc tgctgcagcg ctcctggatg cgcgacgact attcgacgat
     9001 caccaacacc ttcccggacc acgaagactt gcaagggccg gcgggcatca acatcccgca
     9061 ggtcatgacc gagttcattc ccgagaagcg cgtcgtgctg acggctgagg aacagatgcg
     9121 tccgattctg caaagcgccg ccgatgcgct cggcaccgaa ctcaagggcg taggctggct
     9181 ggaatcgggc ctgatcgcgc cggacatcct cgcgcgcttt ccgtatcagg agcacccgga
     9241 caacatcgcg ctggtcgcgg cactcggtac ggagcttggc attgactacg acttctcgct
     9301 caaggcgatg gccgacaagg tggtgccgga cctgggtgta ctgaagacct tcccggtggc
     9361 ccgggtcggc tcgcggcgcc tggagttttc caacggcatg tcggccaatg aacgctatgg
     9421 ctgcctcggc aactgggaac gcctcggcct cgcccggcag gatccgtttg cggaaccggg
     9481 cgtctgcatc agcaccgtcg tcaacaatcg tgccgaccgg gtcgcacgtt cgcgcgtttt
     9541 tgccggcgtg ctgacccagg acatccgggc cgacttccac gtgctgatcg gttccaacct
     9601 ggatggcctg cgcggctaca tcaaggaggc atggcatgcc cacgcgcagg gcctgagcct
     9661 gaagcgcgcg cacgagaccg aggagacgcc cgtgcgcgcg ttggagcgca tcgcgcgcga
     9721 gtatcgggtc gtccatgccg atgcgcatgt gtcggatcgg ctcgccgtca tgctgaaggg
     9781 cataggcgcg ccggaacagc ttgtcgagca cgcgggggat gtcgaggcat tgggcgcggc
     9841 gcttgcgcag caaggcatcg gcaatggcgg cgcactgctc gacttccatg cgcgcgacct
     9901 gcgtaccttg agcgagtacc gcgcgctcga aacgcgctgc cagcgggcag gcgaaaccgc
     9961 cgccgatctg gacgccgcct tgcgcgagca gatggggatc tggttcgagc gccgcatcgt
    10021 cgtggtggag gattactacg cgagcggcga cgccgtcatc gccacgatcc gcgatgccac
    10081 gccgccgggt ttcgtcaatc gcatcatcgg cttgcagaac atcaagggaa ccggcctgga
    10141 ctttgtctac cgctggctcg cctgggagag ctgccacaag gcctgccagg acctgttgtc
    10201 tgccgaccct gcccagcagc gacggggact ggcaagcctg gccagattcc aggagttcgg
    10261 actgctctgc gaaacagaaa ccgaagcggc gctcgccgcg ctgcaggcca cgccccaggg
    10321 acaaaagcag catgtgcgcg ccgagatcaa gaccattgcc gcccggatga cagagcggat
    10381 ggcgcagatt gattcgagtg cctcttcagc acgggtccag ggctggaagg agaaagtgat
    10441 caacttcgtg gagggcttcc tggatgccgg ggacgcggtc aggcgtcgac gccgctccga
    10501 tcggatatac gacgatctcg tgaaccggcg tatcagcgaa gcgcgggcag cactggaatt
    10561 gcaggcgctc acgcgtcgcc agaaaggcgg gtggctgggt gaagcgatct ccgcatgggg
    10621 caagcgcctg tcggagcggt tgcggattcg ggattcggcg gcgaggcggg agcttgggcg
    10681 atcgtctcgg gtgcccgttc ctgaatcggg atcggacgtg agcggcgctc aagccgcatc
    10741 gcgcgcgcag gcccggcccg agcgggtcga atcgtgatgc aatgcgcggg gctgacgtgc
    10801 cggacgccct cggcgtcgga aaagtccgcg cttgcgcatt tccgggcttt gtagcactat
    10861 tagcgccaat gcaccccggc tgtcttgatt ccatgtcctt cgcgcgcttc tatttttact
    10921 tcttttttag ctattccagc cccttggcgg tggaagagcg gtaagcgcgc gagcagatca
    10981 gcaaaccgaa attcctgaca aaaccgccag aaatggcggt tttttcgttt ttgcagcttt
    11041 gatattcaac tttcattcgg agaccaccat gccgcgcacc gacgacttac gcatccggga
    11101 gatgaaggaa ctgatcccgc cctcccactt gatccgcgag ttttcggtat ccgagaaggc
    11161 cgccaacgtg gcggccaacg cgcgcgtggc gctgcaccgc atcctgcatg gacaggatga
    11221 ccggctgatg gtggtgatcg gtccctgctc gatccatgat ccgaaggccg cgctggagta
    11281 tgcgcgccgc ctggtcgagc agcgcgaccg cttttccgcg gacctggaaa tcatcatgcg
    11341 cgtgtatttc gaaaaaccgc gcaccaccgt cggctggaag gggctgatca acgatccgta
    11401 catggacaac agcttccgca tcaacgacgg cctgcgcatg gcgcgcgaac tgctgctgaa
    11461 catcaacgag ctgggcctgc cggccggcac cgaattcctc gacgtgatca gcccgcagta
    11521 tgtggccgac ctgatcagct ggggcgcgat cggcgcgcgc acgaccgaat cccaggtgca
    11581 ccgcgagctg gcttccgggc tgtcctgccc ggtcggcttc aagaacggca ccgacggcaa
    11641 catcaagatc gcggtcgacg cgatcaaggc cgcgtcccag ccgcaccact tcctgtcggt
    11701 gaccaagggc ggccactcgg cgatcgtcgc caccgccggc aacgaggatt gccatatcat
    11761 cctgcgcggc ggcaagacgc cgaactacga cgcggccagc gtggacgcgg cctgcaggga
    11821 catcgcggcc aacgggctgg catcgcgcct gatgatcgac gcctcgcatg cgaacagctc
    11881 gaagaagccc gaaaaccagg tgccggtatg caaggacatc ggcgctcagg tcgccggcgg
    11941 cgatacccgc atcgtcggtg tgatggtcga atcgcacctg gtggccggcc gccaggacct
    12001 ggtgccggga cgcgagctga cctatggcca gtcgatcacc gacggctgca tcggatggga
    12061 agaaagcgtg aaggtgctgg aagtcctcgc ggagtcggtg cgcaagcgtc gtctgcacgg
    12121 cgacgacgag taaaaagggg ccgctgctcg aaaaaaggat cgcggctcga aatgtggttc
    12181 agttgatgca tccctaaccc atggtcgccc gggcgaaagc cggggcccag cgtcttcgac
    12241 tcggcctgac gtccctgggt cccggctttc gccgggacga cgatgattga atgaaccgca
    12301 tttgctcgcc gctcggcgtc aattcagaag cgccgcgtca gcaccatctg cgtgccttcg
    12361 cggcgcattt ccgtccggtt caggaaggac atgcctagca ggatgatggg cagcccctgt
    12421 tcctgcacca ccgcatcgac gccatgcagt tcgatgtcgc cgaccttgac gcggtcgagc
    12481 ctgaccagat acgcggggac cacgccattg gcggtgttca gataccggat cgcgccgttg
    12541 cggtagtcga tgcccagcct gcgcgcatcc gcagccggca gcgcaatcag cgtggcgccg
    12601 gtatcgagca gcatctgcac gctgccg
//
LOCUS       DATLGF010000404        12141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_120900, whole genome shotgun sequence.
ACCESSION   DATLGF010000404 DATLGF010000000
VERSION     DATLGF010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 12141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12141
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120900"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..776)
                     /locus_tag="VM406_12390"
     CDS             complement(69..776)
                     /locus_tag="VM406_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL76807.1"
                     /translation="MLQLENVSVNYGSFRALDNVSIHAKEGELVVLLGANGAGKSSIF
                     LTVSGLNRAATGSIKFKGRELVGMKPSQIVHDGVVHCPEGRKLFPGMSVLKNLMLGAY
                     VHRSDKKGNERRLEEVFELFPILAERRDQAAGSLSGGQQQMVAIGRALMGRPKALLLD
                     EPSLGLAPLVVKQMFEVIEKINKAGTTVLLAEQNAFAALKIASRAYVIENGHIVLEGS
                     RDELLNNEQVRKAYIGA"
     gene            complement(763..1527)
                     /locus_tag="VM406_12395"
     CDS             complement(763..1527)
                     /locus_tag="VM406_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL76808.1"
                     /translation="MLEVTDLSKRFGGLAAVSNVTTTIEKGKINAIIGPNGAGKTTLF
                     NLISGVHKPTSGRIVFNGKDVTGLRADQVARLGMSRTFQTTNLFERATVLDNLIVGHR
                     LRTESGLLDVVFGTKRLKDEERRCREKAREALDFVGLSHIANRVAADISQEERKRVAF
                     ALALSTDPQLVLLDEPAGGVNPEETIGLAELIRKMVRNGLTVCLIEHKMSMIMNLADK
                     ILVLNYGERIAEGTPAEIRSNPLVIEAYLGSEHAAA"
     gene            complement(1520..2536)
                     /locus_tag="VM406_12400"
     CDS             complement(1520..2536)
                     /locus_tag="VM406_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVL76809.1"
                     /translation="MKIFDGKTGWLLLLAAGALFPFFAGNDYHLTVMSMAFIYAIATV
                     GLNLITGYTGQFNLAHAGFMAIGAYTVGILTVDYNVSFWLAFALSGVVAAIIGFFVGL
                     VSLRLKGHFFSIFTLCVGYIMFLLIEKWESLTHGTVGIIGIPAPEPLFGIDFESPRAQ
                     YYLVLFFLALSVWIMHRIVNSLLGRTFMAIRNGDALAESLGINLMRNKVLAFVLSVFY
                     AGMAGGLYAGYVRFLGPGLAGVEHTFDMTMYMLIGGIGTMFGPLFGSISMPWLTQYLQ
                     FLQDYRFVVFGPLLVALVIFLPNGIVGTWLQWRARLAFRKAAAQKTPAAAPLATPSKG
                     GSHA"
     gene            complement(2536..3399)
                     /locus_tag="VM406_12405"
     CDS             complement(2536..3399)
                     /locus_tag="VM406_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVL76810.1"
                     /translation="MELVLQQILNGLTLGGVYSLVALGLTLVYGILHVPNFAHGAFYM
                     AGAYVAFYLMSAYGANYWLAMGGAAIAVAGIAVVSDRLVFHPLRNAPGLHHMIAAIGI
                     LLFLESGAQAIWGADFHRMQTPFTGIMNVFGAVLPVQRLLIIIAAFALVLVLHLFLTR
                     TVTGSTIIAMAQNRDGAALVGIDPNRVAMLTFAISGVLAAVAAVLYAPINLVYPAMGH
                     LVITKAFVIIVLGGMGSVPGAIVGGLIIGFAEAFGAFYISTDYKDIIAFVLLVAILSV
                     RPEGLFSKGAH"
     gene            complement(3798..5090)
                     /locus_tag="VM406_12410"
     CDS             complement(3798..5090)
                     /locus_tag="VM406_12410"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HVL76811.1"
                     /translation="MSISVTCVSTHRVLLSTAFYLARRPVVDRSLDLAAWELLFFDAG
                     VDPAGTDEPLAAAPSVIADVCEYGLGRVLGDAQGVLYVDGAALSGDMFGFLPPDRLVF
                     VLDAAQAAAPGIAQRILELRGRGFRFALAVRDADVRAEAQLSLFDGVRIDVSGMDRAR
                     LAPLCRTYAAAGKRLLAECVNSRQAHALCVELGVDYFQGTFFAAPQAGPHQPLAPMAA
                     AIAQLMSLIASDADSAEIEHRLKSDVALGLCVLRMANAPAFGHHRIESLRQALMVVGR
                     DQLQRWLQLMLYADAARRQAQALPLLAMAAGRARLMELVAQKLKPGNRGIADSAFTVG
                     LMSLMDALFSRPLPALLDEMPVSEDVATALIARAGYLGQLLALATATEWSGGMPVRLP
                     SLLAELKLDSHTLYGLQLAAFEWSDEVVRHARSLPATP"
     gene            5298..8627
                     /locus_tag="VM406_12415"
     CDS             5298..8627
                     /locus_tag="VM406_12415"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HVL76812.1"
                     /translation="MTFPALSIRLLRALALALALLLHGQAALALTLVLDESTLREPVL
                     NLTPYWEVLEDPQHAWSVEEVIEAPLAARFSSPPRRPDSLHFGMNLSAIWLRINVINS
                     TPQVLERRLEIAYAHLHYVDLFEPGIDGYRRIATGSLRPVAERPTEHRNYVFPITFWP
                     HSTTTLYLRIQSNTGIDIPGRLWEPQAFARKSLHEYMGQAVYFGTVLALGLFNLLLFI
                     ALRDRIYFYYVMFAASSILAVLAYSGIGLQFFWQDAENWKIYSSMVSFALNGAAMLLF
                     QRKLLATRVTVPLLDKVMRAFIGLNVVQVVAFIVAHDVMLPIGIVIDAGNMLLALIVA
                     IACLVRGQRSAAIFLAAFGFLVVAALMTAVRSFGVDEIPVFVTTYGIQIGSAVEMLLF
                     SLALADRFNQIRREKEIAQQQLVQSLQRTERELEQRVNERTAELSRANQDLIENRRAL
                     QAAKEVAEDASRMKSAFLANMSHEIRTPMNAVIGMAHLALQTELNGRQRDYVEKIHRA
                     GASLLRLIDDILDLSKIEAGRLTLEQTEFSLHDVLGNVSLVTAQGAADRRLAYSFDIA
                     ADVPVRLRGDPLRLGQVLINLVGNAIKFTERGHVTVRCHCVERDGGRARLQFSVVDSG
                     IGMTSRQCARLFEPFRQADESTTRKYGGTGLGLTISRRLVELMGGRMTVESVPGKGST
                     FSFTVAFALATEGASLPELPQRLHGWHVLIVDRDDAARATLAQLVQSFGMHSDALADP
                     HAAIGQLHLREGAGAYDLVLAEYHVEGVDALARACEAARAKLILVAPFGTDLAPPAGE
                     PTSVALLCKPVDRSLLHDTLIDLLAGQNRRPVRATARALPRFDGRRVLLVEDNEINQQ
                     IAREMLLATGAEVDIAGNGRLALDLLFAAGPNAYDLVLMDLQMPELGGLAAVRRLRAD
                     AAFAALPVIAMTAHATVEEQAECFACGMQDHIAKPIDPDRFYATLARWMAPGTLAAPL
                     TDVMLNEEPIRISGFDVDDALERLAGDVAMYHQMLGLLVTSLADALAELEAAGRSRDR
                     ARLLAAIHAIRGMAANIGAVVLCARAQALEAKAKTHSEDEAGIAELQALCRATIDAVR
                     EGLDQRGATAGRVPDQAQTLG"
     gene            8790..9572
                     /locus_tag="VM406_12420"
     CDS             8790..9572
                     /locus_tag="VM406_12420"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HVL76813.1"
                     /translation="MAHSLPILLYHRIDRSGLSTATAPDDFRRHLQWLKENGWRALDG
                     AQLTHLLRSGKALPKRAFVITFDDGYESLASAAFPILQEFRYSAICFLSTQFIPGARS
                     WKRPREAQVDDAAFLTWDQVRALQSCGVIDFHSHTHTHRRFEECTMEELTEDLAMPRR
                     ILAGELGLPERHFDHLAWPWGRSTPEWRALAHRLGYRYQYTVARQSFERHGPLDDIPR
                     TCFDAAAFPQFQRQIWLQSGSIGRMWNFAYPFGRRLRQITGF"
     gene            complement(9580..10008)
                     /locus_tag="VM406_12425"
     CDS             complement(9580..10008)
                     /locus_tag="VM406_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76814.1"
                     /translation="MFKTGSWVGAGRWPNQDAHPDMWHKPLRGQVLDFCDVRAWANSI
                     YFPEDNPHAGEVMGVALRLREQGLLEGLTPVWWDHGTHRRMTWERSAALRSAEDDLVL
                     WRAAREQRRDEIEHPRRRRRRSLHEFLPPSQRTLALVQRH"
     gene            complement(10075..10479)
                     /locus_tag="VM406_12430"
     CDS             complement(10075..10479)
                     /locus_tag="VM406_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVL76815.1"
                     /translation="MDILVNIDVPDLEQGIAFYTEALPLHLVRRLFDGSVAELSGAPC
                     RLYLLAKEAGSPAASTTTITRGYGRHWTPVHLDFVVDDIEAALRRAQDAGARQETPLQ
                     QLDWGQLVTLSDPFGNGFCLLRFHGDGYPAAS"
     gene            complement(10661..11755)
                     /locus_tag="VM406_12435"
     CDS             complement(10661..11755)
                     /locus_tag="VM406_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HVL76816.1"
                     /translation="MSKSFMRGALTAMAVLPALALTPVTAAAQQQQPLNVGFVYVSPI
                     GEAGWTWQHDLARKEMEKNLGNKVVTKYVESVPEGADSERVIRDLAQQGNKLIFTTSF
                     GYMNPTLKVARQFPDVKFVHLTGYKTDKNVGVANARFYEGRYLAGVLAGKMTKSNIAG
                     YVGAFPIPEVLQGINAFTRGMRSVNPNAEVRVIWVNSWYDPGKERDAAVTLLGQGADV
                     VTHHTDSHAVVQAAEERGRYAIGYHSDMSKFGPKAQLAAVTHHWGHYYTKTAEEVING
                     TWKPTTVWGGIKDGFVKLEAINQAVPADVKQLVANVEKDIVSGKLSPFDGPVVDNTGK
                     VRHQKGPMSDEALNKMDFYVQGVAGKLPGK"
     gene            complement(12041..>12141)
                     /locus_tag="VM406_12440"
     CDS             complement(12041..>12141)
                     /locus_tag="VM406_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL76817.1"
                     /translation="LATIVVLVLISRNPTWIKLNMPASLGKPFRPH"
BASE COUNT         2138 a         3993 c         3917 g         2093 t
ORIGIN      
        1 ctactgcaac atgctcctgc cgttctttgt ttccagaaaa tgtttcccgt tgccgggttg
       61 ggaaccggtc atgcgccgat gtaggccttg cgcacctgct cgttgttcag cagttcgtcg
      121 cgcgagcctt ccagcacgat gtgtccgttc tcgatcacgt aggcgcgcga ggcgatcttc
      181 agcgcggcga acgcgttctg ctcggccagc agcacggtgg tgccggcctt gttgatcttc
      241 tcgatcacct cgaacatctg tttcaccacc agcggggcca gtcccagcga cggttcgtcg
      301 agcagcagcg ccttcggccg gcccatcagc gcgcggccga tcgccaccat ctgctgctga
      361 ccgccggaca gcgagccggc cgcctggtca cgccgctcgg cgaggatagg gaacagctcg
      421 aacacttcct cgagccggcg ctcgttgccc ttcttgtcag agcgatgcac ataggcgccg
      481 agcatcaggt tcttcagcac cgacatgccg ggaaacagct tgcgcccttc cgggcagtgc
      541 acgacgccgt catgcacgat ctgcgaaggc ttcatgccca ccagctcgcg gcccttgaac
      601 ttgatgctgc cggtagccgc gcggttcaag ccggagacgg tcaggaagat cgagctcttg
      661 ccggcgccgt ttgcgcccag cagcaccacc agctcgcctt ccttcgcatg gatgctgacg
      721 ttgtcgagcg cgcggaaact gccgtaattc accgagacgt tctcaagctg cagcatgttc
      781 actcccgaga taggcttcga tgacgagcgg gttggagcgg atctcggcgg gcgtgccctc
      841 cgcgatcctt tcgccgtagt tgagcaccag gatcttgtcc gcgaggttca tgatcatgct
      901 catcttgtgc tcgatcaggc acacggtcag cccgtttctc accatcttgc ggatcagctc
      961 ggccagcccg atcgtttcct ccggattcac gccgccggcc ggctcgtcca gcagcaccag
     1021 ctgcggatcg gtcgacagcg ccagcgcgaa cgcgacgcgc ttgcgctcct cctgcgagat
     1081 gtcggcggcg acgcggttgg caatatgcga gaggccgacg aaatccagcg cctcgcgcgc
     1141 cttctcgcgg cagcggcgct cctcgtcctt cagccgcttc gtcccgaaga ccacgtcgag
     1201 caggccggat tcggtgcgca gccggtggcc gacgatcagg ttgtcgagca cggtggcgcg
     1261 ctcgaacagg ttggtggtct ggaaggtgcg cgacatgccg agcctggcga cctggtcggc
     1321 gcgcaggccg gtaacgtcct tgccgttgaa cacgatgcgc cccgaagtcg gcttgtgcac
     1381 gccggagatc aggttgaaga gggtcgtctt gccggcgccg ttggggccga tgatcgcgtt
     1441 gatcttgccc ttctcgatcg tggtcgtcac attgctcact gcggccaggc cgccgaagcg
     1501 cttcgagaga tcggtgacct caagcatggg aacctccctt ggacggagtc gcgagcggcg
     1561 cggcggccgg cgtcttctgg gccgctgcct tcctgaatgc gaggcgggcg cgccattgca
     1621 gccaggtgcc gacgatgccg ttgggcagga agatcaccag cgccaccagc agcgggccga
     1681 acacgacgaa gcggtagtcc tgcaggaact gcaggtactg cgtcagccac ggcatcgaga
     1741 tcgagccgaa cagcgggccg aacatcgtgc cgatgccgcc gatcagcatg tacatcgtca
     1801 tgtcgaaggt gtgctccacg ccggccagtc ccgggccgag gaagcgcaca tagcccgcat
     1861 acaggccgcc ggccatgccc gcgtagaaca ccgagagcac gaaggccagc accttgttgc
     1921 gcatcaggtt gatgccgagc gattcggcca gcgcgtcgcc gttgcggatc gccatgaagg
     1981 tgcggccgag cagcgagttg acgatgcggt gcatgatcca gaccgacagc gcgaggaaga
     2041 acagcaccag gtagtactgg gcgcgcggcg attcgaagtc gatgccgaac agcggctcgg
     2101 gagcgggaat gccgatgatg ccgacggttc cgtgcgtgag gctctcccac ttctcgatca
     2161 gcaggaacat gatgtagccg acgcacagcg tgaagatcga gaagaaatgg cccttcagcc
     2221 gcagcgacac cagcccgacg aagaaaccga tgatcgcggc caccacgccg gagagcgcga
     2281 acgcgagcca gaacgacacg ttgtagtcga ccgtgaggat gccgaccgtg taggcgccaa
     2341 tcgccatgaa gccggcatgc gcgagattga actggccggt atagccggtg atcaggttca
     2401 ggccgacggt tgcgatcgca tagatgaacg ccatcgacat cacggtcagg tggtagtcgt
     2461 tgccggcgaa gaaagggaac agcgcgccgg cggcgagcag cagcagccag ccggtctttc
     2521 cgtcgaagat cttcatcagt gtgccccctt cgagaacagg ccctcgggac ggaccgagag
     2581 gatcgccacc agcagcacga aggcgatgat gtccttgtag tcggtcgaga tatagaacgc
     2641 gccgaaggct tcggcaaatc cgatgatcag gccgccgacg atcgcgcccg gcacgctgcc
     2701 catgcccccg agcacgatga tcacgaaggc cttggtgatg accaggtggc ccatcgccgg
     2761 atagacgagg ttgatcggcg cgtacagcac cgcggccacg gcggccagca cgccggaaat
     2821 cgcgaaggtc agcatcgcca cccggttcgg atcgatcccg accagcgccg cgccgtcacg
     2881 gttctgcgcc atcgcgatga tggttgaacc ggtcacggtg cgcgtcagga acaggtgcag
     2941 gaccagcacc agcgcgaagg cggcgatgat gatcaacagg cgctgcaccg gcagcaccgc
     3001 cccgaacacg ttcatgatcc ccgtgaaggg cgtctgcatc cggtggaaat ccgctcccca
     3061 gattgcctgg gcgccggatt ccagaaaaag cagtatcccg atcgcggcaa tcatgtggtg
     3121 cagaccgggt gcatttcgca gcggatggaa caccagtcgg tcggagacca cggcgatgcc
     3181 cgccaccgcg atcgcggcgc cgcccatcgc cagccagtag ttggctccgt aggcgctcat
     3241 caggtagaac gcgacgtacg cccctgccat gtagaacgcg ccgtgagcga aattcggcac
     3301 gtgcaggatg ccgtacacca gggtcaggcc gagggccacc aggctgtaca cgccgcccag
     3361 ggtcaggccg ttcaggatct gctgcagaac aagttccaag tcgtcctcca atcggttttt
     3421 ctgtgcgccc cggcagcgag gccgggactg ccgcactccc acctttttag aaaaaacggt
     3481 ttggtgcgtt tctgttttac tcaaacttac cggctgttcg ccggcctgtc aacgaaaacg
     3541 gaattcagtt ttgcggtgca aaactgcatc taacccggct catgaaaatg ggagattgcg
     3601 agccggaaaa cgatcgaaag aacgcagtat ttccacatgg aagttctgca ttgcagaatt
     3661 gaaggatgtc ggaataatag cacgggcgtt cgctagccgt gcaagaaaat gcagcggtgc
     3721 ggacgccggt caaacggaca tcagggtccg gtgcaacgca tgcatgcagg cggaggggac
     3781 actgtgcgga gcggttatca gggcgtcgca ggcaagctgc gcgcatggcg caccacctcg
     3841 tcgctccatt cgaaggcggc cagctgcagg ccgtaaaggg tgtgggagtc gagcttcagt
     3901 tccgccagca gcgacggcag gcgcaccggc atgccgcctg accattcggt ggccgtggcc
     3961 agcgccagca gctggccgag gtagcctgcg cgcgcgatca gtgcggtcgc gacgtcttcg
     4021 gaaaccggca tctcgtccag cagcgccggc agcggccgcg agaacagcgc gtccatcagc
     4081 gacatcagtc cgacggtgaa agccgagtcg gcgatgccgc gattgcccgg cttgagcttc
     4141 tgcgccacca gctccatcag tcgggcccgt ccggcggcca ttgcaagaag cggcagcgcc
     4201 tgcgcttgac gacgggcggc gtccgcatac agcatcagct gcagccagcg ctgcagctga
     4261 tcgcgcccca ccaccatcag ggcctgccgc agcgactcga tgcgatggtg gccgaaggcc
     4321 ggtgcattgg ccatgcgcag cacgcacagg ccaagcgcga cgtcgctctt cagacggtgt
     4381 tcgatctcgg cgctgtccgc gtcggaagcg atcagcgaca tcagctgcgc gatcgccgcc
     4441 gccatcggcg ccagaggctg gtgcgggccg gcctgcggcg cggcgaagaa ggtcccctgg
     4501 aagtaatcga cgccaagctc gacgcatagc gcgtgcgcct gccgggagtt cacacattcg
     4561 gccagcaggc gctttcccgc cgctgcatac gtccggcaca agggcgcgag gcgggcccgg
     4621 tccatgccgc tgacgtcgat gcgcaccccg tcgaacagcg acagctgcgc ctccgcccgt
     4681 acgtcggcgt cgcgcaccgc gagtgcgaat cgaaagccgc gcccgcgcaa ttcgaggatc
     4741 cgctgcgcga tcccgggcgc cgccgcctgt gcggcgtcga gcacgaacac caggcggtcg
     4801 ggcgggagaa aaccgaacat gtcgccggac agcgccgcgc cgtccacgta cagcacccct
     4861 tgcgcgtcgc caagcacgcg tccgagcccg tattcgcaca cgtccgcgat caccgagggc
     4921 gcggcggcca acggttcatc cgtgcccgcc gggtcgacgc cggcgtcaaa gaacagcagc
     4981 tcccatgccg ccagatcgag cgagcggtcc acgaccggcc tgcgcgccag atagaacgcc
     5041 gtcgagagca gcacccgatg cgtactgacg caggtcacgc tgatgctcat gcccatcctc
     5101 ccatgtgcga acgcacatga cgcgctgccg aattcggtca tcacctgccg cgttcgctgc
     5161 cgttatcacg gtttacggcg cccgcacggt cgaacttgag ccgggagacg tatcgtttgc
     5221 gcaacttgac gaaaatcaag ccgtggtaga ttcggccgaa gcgcgcagcc gcgcgcaccg
     5281 cctttcaccc ccgtccgatg acctttcccg ccctgtccat ccgcctgctt cgcgcgctgg
     5341 cgctggcgct cgccctcctt ctgcatggcc aggcagcgct ggcgctgacg ctcgtcctgg
     5401 acgaaagcac gctgcgcgag ccggtactga acctgacacc gtactgggaa gttctggaag
     5461 acccccagca cgcatggagt gtcgaagaag tgatcgaggc gccgctggcc gcccgcttca
     5521 gctcgccgcc gcggcgtccc gactccttgc atttcggcat gaacctgtcg gcgatctggc
     5581 tgcgcatcaa cgtcatcaac agcacgccgc aggtcctgga gcgcaggctc gaaattgcct
     5641 atgcgcatct gcactacgtc gacctttttg aaccgggaat cgacggttat cgccgcattg
     5701 ccaccggctc gctgcggccg gtcgccgagc ggcccaccga gcaccgcaac tacgtgttcc
     5761 cgatcacgtt ctggccgcat agcacgacca cgctctatct gcgcatccag tccaacaccg
     5821 gcatcgacat tcccgggcgt ctctgggagc cgcaggcctt cgcacgcaag agcctgcacg
     5881 aatacatggg acaggctgtg tacttcggca ccgtgctggc gctggggctg ttcaacctgc
     5941 tgctgttcat cgcgctgcgc gacaggatct atttctacta tgtgatgttc gccgccagca
     6001 gcattctcgc agtgctggcc tacagcggaa tcggcctgca gttcttctgg caggacgccg
     6061 agaactggaa aatctactcg tcgatggttt cgttcgcgct gaacggcgcg gcgatgctgc
     6121 tgttccagcg caagctgctg gccacacgcg tcaccgtccc gctgctggac aaggtcatgc
     6181 gcgccttcat cgggctcaac gtcgtgcagg tcgtcgcctt catcgtcgcg cacgacgtga
     6241 tgctgccaat cgggatcgtg atcgacgccg ggaacatgct tctggcgctg atcgtcgcca
     6301 tcgcctgcct ggtgcgggga cagcgcagtg ccgccatctt cctcgccgcc ttcggcttcc
     6361 tcgtggtcgc cgcgctgatg acagcggtgc gcagtttcgg cgtcgatgag atcccggtgt
     6421 tcgtgacgac ctacggcatc cagatcggtt cggccgtgga gatgctgttg ttctcgctcg
     6481 cgctggccga ccgcttcaac cagatccgcc gcgagaagga aatcgcccag cagcagctgg
     6541 tgcagagcct gcaacgtacc gaacgcgagc tggagcaacg ggtgaacgag cgcaccgccg
     6601 agctgtcgcg cgcgaaccag gatctgatcg agaaccggcg cgcgctgcag gcggccaagg
     6661 aggtggcgga ggacgcctcg cgcatgaagt ccgcgttcct cgccaacatg agccacgaga
     6721 tccgcacacc aatgaacgcg gtgatcggca tggcccacct ggcgctacaa accgagctga
     6781 acggcaggca gcgcgactat gtcgaaaaaa ttcatcgcgc cggcgcctcg ctgctgcgcc
     6841 tgatcgacga tatcctcgac ctgtcgaaga tcgaagcggg acggctgacg ctggagcaga
     6901 ccgagttttc gctgcacgat gtgctcggca atgtcagcct ggtcaccgcc cagggcgcgg
     6961 ccgacaggag gctggcctat tcgttcgaca tcgcggccga cgtgccggtg cgcctgcgcg
     7021 gcgacccgct tcggctgggc caggtgctga tcaacctcgt cggcaatgcg atcaagttca
     7081 ccgagcgcgg ccatgtgaca gtgcgttgcc actgcgttga acgcgatgga ggccgcgcaa
     7141 ggctgcagtt ttccgttgtc gacagcggta ttggcatgac ctcgcggcag tgcgcgcgcc
     7201 tgttcgagcc tttccgccag gccgacgaat cgacaacgcg caaatacggc ggaaccgggc
     7261 tggggcttac gatcagccgg cgcctggtgg aactgatggg cggacgcatg acggttgaaa
     7321 gcgtgccggg aaagggctcg acgttcagct tcacggttgc cttcgcgctc gccaccgagg
     7381 gcgcgagcct gcccgaactg ccgcagcggc tgcacggctg gcacgtgctc atcgtcgacc
     7441 gcgatgacgc cgcgcgcgcc acgctcgccc aactggtgca atccttcggc atgcattccg
     7501 acgcgctcgc cgatccccac gctgcgatag gccagctgca cctgcgcgaa ggtgcgggcg
     7561 cttacgacct cgtgctggcc gaataccacg tcgaaggtgt ggatgcgctt gcccgcgcct
     7621 gcgaggccgc gcgcgccaag ctgatcctgg tcgcgccgtt cggaaccgac ctcgcgccgc
     7681 cggccggcga accgacttcg gtcgcactgc tgtgcaagcc ggtcgaccgc tccctgctgc
     7741 acgacacgct gatcgacctg cttgcggggc agaaccgcag gccggtgcgt gcgacggcgc
     7801 gtgcgctgcc gcgcttcgac ggccggcgcg tgctgctggt cgaggacaac gagatcaatc
     7861 aacagatcgc gcgcgagatg ctgctcgcca ccggcgccga ggtggacatc gcgggcaacg
     7921 gcaggctggc gctggatctg ctgtttgccg ccggccccaa cgcctacgat ctcgtgctga
     7981 tggatctgca gatgccggaa ctgggcgggc tggcggcggt acgccgcctg cgcgccgacg
     8041 cggccttcgc ggcgctgccg gtcatcgcga tgaccgccca cgcgaccgtc gaggaacagg
     8101 cggaatgctt tgcatgcggc atgcaggacc acatcgccaa gccgatcgac cctgaccgct
     8161 tctatgcgac gctggcgcgc tggatggcgc ccggcacgct ggccgcgccc ctcaccgacg
     8221 tgatgctgaa cgaggaaccg atccgaattt ccggatttga tgtcgacgac gcattggagc
     8281 ggctcgccgg cgatgtcgcg atgtatcacc agatgcttgg cctgctggtg accagcctgg
     8341 ccgacgccct ggcggaactg gaggcggctg gcaggagccg ggaccgggcg cgcctgcttg
     8401 cggccatcca cgcgattcgc gggatggcgg ccaacatcgg cgcagtcgtg ctgtgcgcgc
     8461 gcgcgcaggc actggaagca aaggcgaaga cgcactcgga agacgaggca gggatcgcgg
     8521 agctgcaggc gctgtgccgc gcaacgattg acgcggtacg cgagggactc gatcagcgcg
     8581 gcgcaacagc cggacgcgta ccggatcagg ctcaaacgct tggttgactg gacacaattt
     8641 aacgggcggc aggataaccg atagtagaac cgagttcgca tggccatgtc ggacgttgta
     8701 gtgaaccctg gttccggtga agaccggtct gcccgcgcgt tgcgggcgtg tgcggcgtgc
     8761 gccgtcgcac tgttgggaga tccatgccga tggcacattc gcttccgatt ctgctgtacc
     8821 accgcatcga ccggtccggc ctgtcgaccg cgaccgcccc ggatgatttc cggcgccacc
     8881 tgcagtggct gaaggagaac ggctggcggg cgctggatgg cgcgcaactc acgcatctgc
     8941 tccgttccgg gaaagcgctg cccaagcgcg cgttcgtgat caccttcgat gacggctacg
     9001 aaagcctcgc ctcggccgca ttccccatcc tgcaggagtt ccgttattcg gcgatctgct
     9061 tcctgtccac gcaattcatc cccggcgcgc gcagctggaa gcgtccgcgc gaagcccagg
     9121 tggacgacgc cgcctttctc acctgggacc aggtccgcgc attgcagtcc tgcggcgtga
     9181 tcgacttcca ttcgcacacc cacacgcatc ggcgtttcga ggaatgcacg atggaggaac
     9241 tgaccgagga cctggccatg ccgcgccgga ttctggccgg cgaacttggc ctgccggagc
     9301 gtcacttcga ccacctggcg tggccctggg ggcgctccac ccctgagtgg cgcgcgctgg
     9361 cgcacaggct gggctatcgc taccagtaca cggtcgcgcg ccaatcgttc gagcggcacg
     9421 ggccgctgga cgacatcccc cgtacctgct tcgacgctgc cgccttcccg caattccagc
     9481 gccagatctg gctgcagtcc ggctcgatcg gacgcatgtg gaacttcgcc tatccgttcg
     9541 gccggcgcct gcggcagatc accggattct gaactgccct cagtggcgct gcaccagcgc
     9601 gagcgtgcgc tgcgacggcg gcaggaactc gtgcaggctg cgccgtcggc gccggcgcgg
     9661 gtgttcgatc tcgtcgcggc gctgctcccg cgccgcgcgc cacagcacca gatcgtcctc
     9721 ggcgctgcgc agcgctgcgc tgcgctccca tgtcatgcgc cggtgcgtgc cgtgatccca
     9781 ccagactggc gtgagcccct ccagcaggcc ttgctcgcgc aagcgcaagg ccacccccat
     9841 cacctcgccg gcatgcggat tgtcttcagg aaaatagatc gagttcgccc aggcgcgcac
     9901 gtcgcaaaaa tccagcacct ggccgcgcag cggcttgtgc cacatatcgg gatgggcgtc
     9961 ctggttcggc cagcgtccgg cgccgaccca gcttcctgtc ttgaacatga tgacctcctc
    10021 ggcgccatcg ccaagcgcca tgactgtgat tatatacagt attcgggcat ctgctcagga
    10081 agccgcagga tagccgtccc catggaagcg cagcaggcaa aagccgttgc cgaacggatc
    10141 cgaaagagtc acgagctggc cccagtccag ctgctgcagc ggcgtttcct ggcgcgcgcc
    10201 ggcatcttgt gcgcgtcgca gcgcggcctc gatgtcgtcg acgacgaagt cgagatggac
    10261 cggcgtccaa tggcggccat atccgcgcgt gatcgtggtg gttgaggccg ccgggctccc
    10321 ggcttccttg gccagcaggt aaaggcggca gggcgcgccc gacagctccg ccacgctgcc
    10381 gtcgaacagg cggcggacga ggtgcagcgg cagggcctcg gtgtagaagg caatgccctg
    10441 ctccaggtcg ggaacatcga tgttgacgag aatgtccatg gcagccgtgc tttggcctgc
    10501 ggattcgcaa atgccgtcca agttccgcag cgtccgtgct gcttgcgctg caacggccat
    10561 gcgagcggca ttgccatctg gcagtcggtt cgaaaaaccg gcaggccaga aaaaaggccc
    10621 gcttctgcgg gccctttcct ggtgcgaaat ggctgcgtga ttatttgccc ggcagcttgc
    10681 cggcgacgcc ctggacatag aaatccatct tgttcagcgc ttcatcggac atcggtccct
    10741 tctggtggcg gaccttgccg gtgttgtcga ccaccggacc gtcgaacggg ctgagcttgc
    10801 cggacacgat gtctttctcg acgttcgcca ccagctgctt cacgtcggcc ggcacggcct
    10861 ggttgatcgc ttccagcttc acgaagccgt ccttgatgcc gccccagacg gtggtcggct
    10921 tccaggtccc gttgatgact tcctcggcgg tcttcgtgta gtagtggccc cagtggtggg
    10981 tcaccgcggc gagctgcgcc ttcggaccga acttgctcat gtcggagtgg tagccgatcg
    11041 cgtagcggcc gcgctcttcc gccgcctgca ccaccgcgtg cgagtcggtg tggtgggtca
    11101 cgacatcggc accctggccc agcagggtga cggcggcgtc gcgctccttg cccgggtcat
    11161 accagctgtt gacccagatc acgcgcactt cggcgttcgg attcacgctg cgcatgccgc
    11221 gggtgaaggc gttgatgccc tgcagcactt cgggaatcgg gaacgcgccg acgtagccgg
    11281 cgatgttcga cttggtcatc ttgccggcca gcacgccagc caggtagcgg ccttcgtaga
    11341 agcgggcatt ggccacgccg acgttcttgt cggtcttgta gccggtcagg tggacgaact
    11401 tcacgtccgg gaactggcgt gcgactttca gcgtgggatt catgtagccg aacgaggtcg
    11461 tgaagatcag cttgttgccc tgctgcgcga ggtcgcggat cacgcgttcg gagtccgcgc
    11521 cctcgggcac gctttccacg tacttggtga cgaccttgtt gccgaggttc ttttccatct
    11581 ccttgcgggc aagatcgtgc tgccaggtcc agccggcttc gccgatcggg ctgacgtaga
    11641 cgaagccgac gttcagcggc tgctgctgct gagcagcggc ggtaacgggg gtgagcgcca
    11701 gtgccggcag cacggccatg gcggtcaggg cgccgcgcat gaagctttta gacatgtttt
    11761 tcctctacac gtaaacccac tgatatcgac cagcacgccg agtgggaaac ggcgcactgc
    11821 cggagcgcct gcgacgggtt caggcacccc gctccagttc cgcgggaccg ctggtcacga
    11881 cccctcgatc ttcggacttc caaaagcgca ttgccagcct tgcgatgcaa gaccgaaaac
    11941 tattaccacg gcgggaatcc gtctgatata tggcgccgcg aatagcgagc atattgccag
    12001 acaaattccc gcgcgtcaga ggcggtgagc ggcttcgtca tcaatgcggc cggaacggct
    12061 tgccgagcga ggccggcatg ttgagcttga tccaggtcgg attgcgcgag atcagcacca
    12121 gcacgacgat ggttgcgagg t
//
LOCUS       DATLGF010000405         2784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_120912, whole genome shotgun sequence.
ACCESSION   DATLGF010000405 DATLGF010000000
VERSION     DATLGF010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120912"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1619
                     /locus_tag="VM406_12445"
     CDS             <1..1619
                     /locus_tag="VM406_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HVL76818.1"
                     /translation="KLCNLKELIEVFLQHRREVVTRRTVFNLRKARERGHVLEGLAVA
                     LANIDEFIRIIRESPTPPVAKTELMHRSWDSSLVREMLTRAREDGSRVSADDYRPDGL
                     DREYGMQADGLYRLSDTQAQEILQMRLQRLTGLEQDKIVAEYRDVMAEIEDLLDILAK
                     PQRVSVIIGEELTALKQEFGQTKIGARRSEIEHNAQELATEDLITPTDMVVTLSHAGY
                     IKSQPLSEYRSQKRGGRGKQATATKEDDWIDQLFIANTHDYILCFSNRGRLYWLKVWE
                     VPQGSRNSRGKPIVNMFPLQDGEKITVVLPLSGENRTFPEDHYVFMATSLGTVKKTPL
                     TEFSNPRKAGIIAVDLDEGDYLIGAALTDGEHDVMLFSDGGKAVRFDENDVRPMGRTA
                     RGVRGMNLEEGQQVIALLVAENEHQSVLTATANGYGKRTPITEYTRHGRGTKGMIAIQ
                     TSERNGKVVAATLVEPHHEIMLITTGGVLIRTRVSEIRQMGRATQGVTLIAVEDGTKL
                     RGLQRVVEADADEEIAAAEGGEESGGAEEA"
     gene            1616..2707
                     /gene="serC"
                     /locus_tag="VM406_12450"
     CDS             1616..2707
                     /gene="serC"
                     /locus_tag="VM406_12450"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HVL76819.1"
                     /translation="MTRVYNFSAGPAALPEEVLRQAADEMLDWHGSGMSVMEMSHRGR
                     EFMSIIEAAERDLRELLNVPANYRILFLQGGGIGENAIVPLNLVGRKPQPATIDFIHT
                     GSWSGKSIKEARKYANVNVAASSEAVKFSAIPAREEWQLTGDAAYVHICTNETIDGVE
                     FNYAPDTGGVPLVADMSSHILSREVDVSKYGVIYGGAQKNIGPAGVTLVIVREDLLGH
                     ALGICPSAFDWKVVADNHSMYNTPPTYGIYIAGLVFQWLKRQGGVAAIERRNIAKAAL
                     LYGYLDSTGFYSNVIAADCRSRMNVPFFLRDESLNDAFLAGAKERGLVQLKGHKSVGG
                     MRASIYNAMPVEGVQALVDYMKDFEKRFG"
BASE COUNT          545 a          863 c          917 g          459 t
ORIGIN      
        1 cgaagctgtg caacctcaag gagctgatcg aggtcttcct gcagcaccgg cgcgaggtcg
       61 tcacccggcg taccgtgttc aacctgcgca aggcgcgcga acgcgggcat gtgctggaag
      121 gcctggcggt ggcactggcg aacatcgacg agttcatccg catcattcgg gagtcgccca
      181 cgcctcccgt ggccaagacg gagctgatgc accgcagctg ggactccagc ctggtgcgcg
      241 agatgctcac gcgggcccgc gaggacggct cgcgcgtcag tgcggacgac taccggccgg
      301 atgggctgga ccgcgagtac ggcatgcagg cagacggctt gtaccgcctg tcggatacgc
      361 aggcgcagga aatcctgcag atgcggctgc agcgcctgac cggcctcgag caggacaaga
      421 tcgtcgccga ataccgcgac gtcatggccg agatcgagga cctgctcgac atcctggcca
      481 aaccccagcg ggtgtccgtc atcatcggcg aggaactcac cgctctgaag caggagttcg
      541 gccagaccaa gatcggggcg cgtcgcagcg aaatcgagca taacgcgcag gagctcgcga
      601 ccgaggatct gatcacgccc acggacatgg tggtgacgct gtcgcatgcc ggctatatca
      661 agagccagcc cttgtcagag taccgctcgc aaaagcgcgg cggacgcggc aagcaggcca
      721 cggcaaccaa ggaagacgac tggatcgacc agctcttcat cgccaacacc catgactaca
      781 tcctgtgctt ttccaaccgc ggccgcctct actggctgaa agtgtgggaa gtgccgcagg
      841 gttcgcgcaa ttcgcgcggc aagccgatcg tcaacatgtt cccgctgcag gatggcgaaa
      901 agattaccgt cgtgctgccg ctgtcgggcg agaatcgcac cttccctgaa gaccattacg
      961 tgttcatggc gacgtcgctt ggcacggtca agaagacccc gctgaccgaa ttctccaatc
     1021 cgcgcaaggc cggcatcatc gcggtcgacc tcgacgaggg cgactacctg atcggcgcgg
     1081 cactgaccga cggcgagcat gacgtgatgc tgttctccga cggcggcaag gcggtgcgct
     1141 tcgacgagaa cgacgtgcgt ccgatgggcc gcaccgcgcg cggcgtgcgc ggcatgaacc
     1201 tggaggaagg ccagcaggtg atcgcgctgc tggtggccga gaacgagcac cagtcggtgt
     1261 tgacggcgac cgcgaacggc tacggcaagc gcacgccgat caccgaatac acgcgccacg
     1321 ggcgcggcac caagggcatg atcgcgatcc agacctccga gcgcaacggt aaggtggtcg
     1381 ccgccacgct ggtcgagccg caccatgaaa tcatgctgat taccaccggc ggcgtgctga
     1441 tccgcacccg cgtctccgag atccgccaga tgggccgcgc gacccagggc gtgaccctga
     1501 tcgccgtcga ggacggcacc aagctgcgcg ggctgcagcg ggtggtggag gccgatgccg
     1561 atgaggagat tgcggcggcc gagggcggcg aggaatcggg cggcgcggag gaagcatgac
     1621 gcgcgtctac aatttctcgg cgggtccggc agcattgccg gaagaggtgc tgcgccaggc
     1681 cgccgatgaa atgctggact ggcacggcag cggcatgtcg gtgatggaaa tgagccatcg
     1741 cggccgcgaa ttcatgtcca tcatcgaagc ggccgaacgc gacctgcgcg aactgctgaa
     1801 cgtgcctgcc aactaccgca tcctgtttct gcagggcggc gggattggcg agaacgcgat
     1861 cgttccgctg aacctggtcg gccgcaaacc gcagccggca accatcgatt tcatccacac
     1921 cggctcctgg tccggcaagt cgatcaagga agcgcgcaag tacgcgaacg tcaacgttgc
     1981 cgccagttcc gaggccgtaa agttctcggc cattcccgcg cgcgaggaat ggcagctcac
     2041 cggcgacgcg gcctatgtgc atatctgcac gaacgagacc attgacggcg tcgaattcaa
     2101 ctatgcgccc gatacaggcg gggttccgct ggtggcggac atgtcctcgc acatcctgtc
     2161 gcgtgaggtc gacgtatcga agtacggcgt catctatggc ggcgcccaga agaacatcgg
     2221 accggctggc gtgacgctgg tgatcgtgcg tgaagacctg ctcggccatg ctcttggaat
     2281 ctgcccgtcg gcattcgact ggaaggtcgt cgctgacaat cactcgatgt acaacacgcc
     2341 gccaacctac ggcatttata tcgccggact ggtgttccag tggttgaagc gccagggcgg
     2401 cgtggccgca atcgagcggc gcaatatcgc caaggccgcg ctgctgtatg gctatctcga
     2461 ttccaccggg ttttactcga atgtcattgc ggccgattgc cgctcgcgca tgaacgtgcc
     2521 gttcttcctg cgcgacgagt cgctgaatga tgcgttcctc gccggtgcga aggagcgcgg
     2581 tctggtccag ctgaagggtc acaagtccgt aggcggcatg cgggcatcga tctacaacgc
     2641 gatgccggtc gagggcgtac aggcgctcgt cgattacatg aaggacttcg agaaacgctt
     2701 cggataagct cccttcccct tgcaggggag ggccagggtg gggatgtcgt cactgcgcga
     2761 cggcggtgtt cgatgtttcc atcc
//
LOCUS       DATLGF010000406         5477 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_198709, whole genome shotgun sequence.
ACCESSION   DATLGF010000406 DATLGF010000000
VERSION     DATLGF010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198709"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VM406_12455"
     CDS             complement(<1..195)
                     /locus_tag="VM406_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine peptidase"
                     /protein_id="HVL76820.1"
                     /translation="MKAIQLLRNAFFAVLFGSAVALLAPAGGGVIASAHAQQGQILPD
                     FADLAERTGPAVVNIRTTQRM"
     gene            complement(208..1218)
                     /locus_tag="VM406_12460"
     CDS             complement(208..1218)
                     /locus_tag="VM406_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HVL76821.1"
                     /translation="MRQTLTAWLKFAVALSVLVSLTSRADNAGGTPEAQAWLTKIQTA
                     AQRLNYSGTFVYQQGSQMRTSRITHLLEGGTEIEKLEILDGNPREYIRKNDEVICYVP
                     ETRTLQVEKRVSRDAFPAILGASPAELAQHYHIRKAETGRIAGHDCQAIILEPKDKLR
                     YGYKLWAEKSSGLLLRAQTLNDRAEVVEQIAFTQVAIGNVDRSRVNPSYVNTAGWRVE
                     HAVTTPANLHGWTVGGLPPGFRKVRELKRVVSDGPADGATNVPQTAGREIGQMIFSDG
                     LAAISVFIEPGSYSRTEGTLQQGALNILGKRYGDYWLTIVGEVPSAAIRQVANSIEYQ
                     PK"
     gene            complement(1224..1772)
                     /locus_tag="VM406_12465"
     CDS             complement(1224..1772)
                     /locus_tag="VM406_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HVL76822.1"
                     /translation="MNTNDMSRERVSALADGELEEAELQTVLAALRADETKADWALYH
                     QIGDILRSDEMAAVRLSSGFSERMAARLEAEPTVIAPASVRRGRSRLLARAAAAAAVA
                     GFAFIITPPMIDAFQGVEVQPAPLAEVAPSGPPDVVLTAAPEGVILRDPRIDEYLLAH
                     QRFSPSVYSSAQHARAAAFSGK"
     gene            complement(1829..2431)
                     /gene="rpoE"
                     /locus_tag="VM406_12470"
     CDS             complement(1829..2431)
                     /gene="rpoE"
                     /locus_tag="VM406_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HVL76823.1"
                     /translation="MTTERDIDQLLVERVQRGDKRAFELLVTKYQRKLMRLVSRLVRD
                     QAEAEDVVQEAFIKAYRALPQFRGDSAFYTWLYRIGINTAKNYLVTQGRRAPTSTEAD
                     AEEAETFDDGDHLRDINTPESMLATKQIAETVNVAMDALPDELRIAITLREIEGLSYD
                     EIAEAMGCPIGTVRSRIFRAREAIAEKLRPLLGTAIDKRW"
     gene            complement(2529..3026)
                     /locus_tag="VM406_12475"
     CDS             complement(2529..3026)
                     /locus_tag="VM406_12475"
                     /inference="COORDINATES: protein motif:HMM:NF018908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HVL76824.1"
                     /translation="MSIAVSAVVKPSRLLFLVILGMSFLSGAVAILIGLGALGAALSM
                     LARFVLVVVCGFTAGFGFYHGICQRKTIHMEITRGGQIRIREAVVERSCGEANRPHVE
                     EAEAVRLMPESTIWPQLLLLRLQAESGKITSLAILPDSVRGDTFRALAASCRWIAARY
                     DRSSG"
     gene            complement(3088..4335)
                     /gene="fabF"
                     /locus_tag="VM406_12480"
     CDS             complement(3088..4335)
                     /gene="fabF"
                     /locus_tag="VM406_12480"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HVL76825.1"
                     /translation="MSRTRERRVVVTGLGCISPVGNTVADSWAALTEGKSGIATITKF
                     DATPFSTRFAGEVKGFNIEDYIPGKEARHMDTFIHYGMAAGMQAIRDSGLTVTEQNAE
                     RIGVLVGSGIGGLPMIEETHAELTNRGPRRISPFFVPASIINMISGHLSIKYGLKGPN
                     LAIVTACTTGLHCIGAAARMIEYGDADVMIAGGAESTVSPLGIGGFAAARALSVRNDD
                     PATASRPWDKDRDGFVLGEGAGVMVLEEYEHAVARGATIYAEVLGFGMSADAYHMTAP
                     LEDGDGARRCMVAAMNNARINPDQVDYVNAHGTSTPLGDIAETVAIKRALGDHAKDVV
                     VNSTKSMTGHLLGGAGGLESVFTVLAVHHQISPPTINIFNQDPACDLDYCANTAREMP
                     IRVAVKNSFGFGGTNGTLVFGKV"
     gene            complement(4367..4606)
                     /gene="acpP"
                     /locus_tag="VM406_12485"
     CDS             complement(4367..4606)
                     /gene="acpP"
                     /locus_tag="VM406_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012735022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HVL76826.1"
                     /translation="MSDIEQRVKKIVAEQLGVAEADIKNESSFVDDLGADSLDTVELV
                     MALEDEFEMEIPDEQAEKITTVQQAIDYAKAHVKA"
     gene            complement(4734..>5477)
                     /gene="fabG"
                     /locus_tag="VM406_12490"
     CDS             complement(4734..>5477)
                     /gene="fabG"
                     /locus_tag="VM406_12490"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HVL76827.1"
                     /translation="NLTNQVALVTGASRGIGRAIAVELARQGAKVVGTATSEAGAAAI
                     SDYLAAVAPECGKGLVLNVTDAERCNAVIEQVQKEYGSLSILVNNAGITQDQLAMRMK
                     DEEWDAVIATNLSAVGRLCRGVLRGMMKARFGRIINITSVVGSAGNPGQMNYAAAKAG
                     VAGMSRALAREIGSRNITVNCVAPGFIDTDMTRELGEQQVAALMQQIPLARFGQPEDI
                     AHAVAFLASPQAGYITGTTLHVNGGMYMA"
BASE COUNT         1011 a         1732 c         1647 g         1087 t
ORIGIN      
        1 catgcgctgt gtcgtgcgga tattgacgac cgccgggcca gtacgctccg ccaagtccgc
       61 gaaatccgga agtatctgcc cctgctgcgc atgggctgaa gcgatcacgc ccccgcctgc
      121 gggagccaga agcgcaacgg ctgagccgaa cagcacagcg aaaaaggcat tgcgcaagag
      181 ctggattgct ttcatcggat cctcttctta ttttggttga tactcgattg aattcgccac
      241 ttgccggatc gcagcggacg gcacctcccc gacgatggtg agccagtaat cgccatagcg
      301 cttgcccagg atattcagcg ccccctgctg cagggtgcct tcagtccggc tgtaggagcc
      361 gggctcgatg aacacggaaa tggcggcaag gccgtccgag aaaatcatct gtcctatttc
      421 acgcccggcg gtctgcggca cattggttgc cccgtcggcc ggcccgtcgc tgaccacgcg
      481 cttgagttcg cgcaccttgc gaaagcctgg cggcagtccg ccgaccgtcc agccgtgcag
      541 attcgccggc gtcgtcaccg catgctcgac gcgccagccg gcagtgttca cgtagcttgg
      601 attgactcgg ctgcgatcca cgttcccgat cgcgacctgg gtgaacgcaa tctgctctac
      661 gacctccgct ctatcgttga gcgtctgggc ccgcagcagc agcccggaag acttctccgc
      721 ccacagcttg tatccatagc gaagcttgtc cttgggttca aggatgatgg cttggcagtc
      781 gtgtccggct atgcgtccgg tttccgcctt gcggatatgg taatgctgcg ccagttcagc
      841 cggcgacgcg ccgagaatcg ccggaaatgc gtcgcggctg acgcgctttt ccacctgcaa
      901 ggtacgcgtt tccgggacat agcagatcac ctcgtcattc tttcggatgt attcgcgcgg
      961 attaccgtcc agaatctcga gcttctcgat ctcggttccg ccctcgagaa ggtgcgtgat
     1021 gcgcgaggtg cgcatctgac tgccctgctg gtagacgaag gttccggagt aattcagtcg
     1081 ctgcgccgcc gtctggatct tcgttagcca ggcttgcgcc tcgggcgtgc cccccgcatt
     1141 gtccgcgcgc gaagtgaggc ttaccaatac cgagagggca acggcgaact tgagccatgc
     1201 cgtcaatgtc tgccgcatcg ctttcattta cccgagaatg ctgccgcgcg cgcgtgctgg
     1261 gcgctgctgt acaccgaagg agagaaacgc tgatgggcga gcaggtactc gtcgatccgc
     1321 gggtcgcgca ggatcacacc ttcgggcgcc gctgtcagca ccacgtccgg tgggccggaa
     1381 ggtgcgacct cggccagggg tgccggttgc acctcgactc cctggaacgc gtcgatcatc
     1441 ggaggcgtga tgatgaacgc aaaacccgcg accgcggctg cggccgcggc ccgcgcgagc
     1501 aggcgactgc gcccacggcg cacggatgcg ggcgcaatga cggtgggctc ggcctcaaga
     1561 cgcgcggcca tgcgctccga aaaaccggaa ctcagacgca ccgcggccat ctcatcggaa
     1621 cgaagaatgt caccgatctg atgatagagc gcccagtcgg ccttggtttc gtccgcgcgc
     1681 agtgcggcga gcaccgtctg taattcggcc tcctcgagtt cgccgtcggc caaggccgaa
     1741 acccgctctc gggacatgtc gttcgtattc ataccatcct tgcgctggcc gcgccctgtt
     1801 tacaaaacca tccaaataac accgctggct accagcgctt gtcgatcgcc gtgccgagca
     1861 gaggcctaag cttttccgcg atcgcctcgc gggcacggaa aatgcggctt cgcacagttc
     1921 cgatcgggca acccattgcc tcggcgattt catcgtagct caagccttcg atctcgcgca
     1981 atgtaatcgc tatgcgcagt tcgtccggca gtgcgtccat cgccacattt accgtctcgg
     2041 caatctgctt ggtggcgagc attgactccg gcgtattgat atcacgaaga tgatcgccgt
     2101 catcaaaagt ctcggcctct tccgcgtcgg cctcggtgga cgtgggcgcc cgccggcctt
     2161 gcgtgaccag gtagttcttg gcagtgttga tgccaatccg atacagccat gtatagaaag
     2221 ccgagtcgcc gcgaaactgc ggcagtgccc ggtacgcctt gatgaaggct tcctgcacca
     2281 cgtcttcggc ttccgcctga tcgcgcacga gccgcgaaac caaccgcatc aacttgcgct
     2341 gatacttggt caccaacagc tcgaatgccc ttttatcgcc acgctgcaca cgctcgacaa
     2401 gcagctgatc tatgtcgcgt tctgtcgtca atcccggttt cctcgtctca ttgcaacata
     2461 cacgcggcgt gagtagttga ccgtgggaaa tgtaagaagt tcaatcgccg ttccgatttt
     2521 tcgtcgcatc atcctgacga gcggtcgtag cgggcggcaa tccaccggca cgacgcggca
     2581 agcgcccgaa acgtatctcc ccggacactg tccggcaaaa tcgccaagga tgtaattttt
     2641 ccgctttccg cctgcaatct gagcaacagc agctgcggcc agatggttga ctcgggcatc
     2701 aaacggactg cttccgcctc ctctacatgc ggccgattgg cttcaccaca agatctttcg
     2761 actaccgcct cccggatccg aatctgcccg ccgcgggtga tctccatatg tatggttttt
     2821 cgttggcaga tgccatgata aaacccaaag ccggcagtga atccgcacac caccacgagc
     2881 acgaaacggg ccagcatcga aagcgcggct ccgagggcgc cgaggccaat gagaatggcg
     2941 acggcgccgc tcaagaacga catgccgagg atgacgagga aaagcagccg agatggcttg
     3001 acgacggcgg agaccgctat cgacatcgca gaaagccggg gatgtttacg tgacggccct
     3061 tcacttgcgc gaggggccgc acgcggttca gaccttcccg aacaccagcg taccgttggt
     3121 gccgccgaag ccgaaggaat tcttgactgc cacgcggatc ggcatttcac gtgcggtatt
     3181 cgcgcagtaa tccagatcgc aggccgggtc ctggttgaag atattgatcg tcggtggaga
     3241 gatctggtga tgcaccgcga gcacggtgaa taccgactcc aggccgccag cgccaccgag
     3301 caggtgcccg gtcatcgact tggtcgaatt gaccacgaca tccttggcgt gatccccaag
     3361 cgcgcgcttg atcgcgaccg tttcggcaat gtctccgagc ggcgtcgagg taccgtgcgc
     3421 attgacgtaa tcgacctgat cgggattgat ccgggcgtta ttcattgcag ccaccatgca
     3481 gcggcgcgcg ccatcgccat cctcgagcgg cgcggtcatg tggtaggcgt cggcgctcat
     3541 accgaagccg agcacttcgg cgtaaatcgt ggccccacgc gctactgcat gctcgtattc
     3601 ttcgagcacc atgacgccgg caccctcgcc gagcacgaat ccgtcgcggt ccttgtccca
     3661 gggccgcgaa gcggtggccg gatcgtcatt gcgcacggac agcgctcgcg cagcggcgaa
     3721 cccgccgatg cccagcggcg acaccgtcga ttcggcaccg ccggcgatca tgacgtcggc
     3781 atcgccgtac tcgatcatgc gggcggcagc gccgatgcaa tgcagtccgg tggtacacgc
     3841 ggtgacgatc gcaagattcg gccccttcaa gccgtacttg atcgacagat ggcccgagat
     3901 catgttgatg atcgaagccg gaacgaagaa cgggctgatg cggcgcggac cacgattggt
     3961 cagttcggcg tgcgtctcct cgatcatcgg cagtccgccg atgccggagc cgacgagtac
     4021 gccgatgcgc tccgcgttct gttcggtcac cgtcaggccg ctgtcccgga tcgcctgcat
     4081 gcccgcggcc atgccgtagt ggatgaaggt gtccatgtga cgcgcctcct taccggggat
     4141 gtagtcctcg atattgaagc ccttcacttc accggcgaag cgcgtggaga aaggcgtggc
     4201 gtcgaacttg gtgatggttg caatgccgga cttgccctcg gtgagcgctg cccaggaatc
     4261 ggcaacggta tttcccacgg gggaaatgca acccaaccct gtcactacga cgcgacgttc
     4321 acgtgtgcgg ctcaaacggt tctcctgaaa ctagatcaat gcgagatcag gccttcacgt
     4381 gtgccttggc gtagtcgatt gcctgctgca cagtggtgat cttctcggcc tgctcgtcgg
     4441 ggatttccat ctcgaattcg tcttcgaggg ccatgacgag ttcaacggtg tcaagcgaat
     4501 cggcaccgag gtcatcgacg aaggacgatt cgttcttgat gtccgcttcg gcgacgccca
     4561 gttgctcggc gacgatcttc ttaacgcgtt gttcgatgtc cgacatatta tccctccagt
     4621 gggttacaga aaagtgcgcg cattttagca ggtttgcgct ttgaaaattc actttcaagc
     4681 cgcgttttca ccgagggcgg aacgcgtcga aagtgcgttg aatccggccg aattcaggcc
     4741 atgtacatgc cgccgttcac atgcagcgtc gtgccggtga tgtagcccgc ttgcggggac
     4801 gcaaggaagg cgaccgcatg agcgatgtcc tccggctggc cgaagcgcgc cagcggaatc
     4861 tgctgcatga gggccgcgac ctgttgctcg ccgagttcgc gcgtcatgtc cgtatcgatg
     4921 aatcccggtg ccacgcagtt gaccgtgatg ttgcggctgc cgatctcgcg cgccagcgcg
     4981 cggctcatgc cggccacgcc cgccttggcg gcggcgtagt tcatctgacc cgggttgccg
     5041 gccgatccga ccaccgaggt gatattgatg atgcggccaa agcgcgcctt catcatgccg
     5101 cgcaggacgc cgcggcacag gcgcccgact gcgctcaggt tggtcgcgat taccgcatcc
     5161 cactcctcgt ccttcatgcg catcgcaagc tggtcctgcg tgatgccggc attgttgacg
     5221 agaatcgaaa gcgagccgta ctccttctgg acctgttcga tcaccgcgtt gcagcgctcg
     5281 gcatcggtga cattgagcac cagccccttg ccgcactccg gcgcaacggc ggcaagatag
     5341 tcgctgatcg cggcggctcc ggcttcggag gtcgcggtcc cgactacctt cgcgccctgg
     5401 cgagcgagtt cgactgcgat ggcgcggccg atgccgcgag aggcgccggt gaccagcgcg
     5461 acttgattgg tcagatt
//
LOCUS       DATLGF010000407         4860 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_172524, whole genome shotgun sequence.
ACCESSION   DATLGF010000407 DATLGF010000000
VERSION     DATLGF010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172524"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="VM406_12495"
     CDS             complement(<1..1261)
                     /locus_tag="VM406_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HVL76828.1"
                     /translation="MSFAELGLSEEIVRAVTEQGYHTPTPIQAQAVPAVLKGGDLLAG
                     AQTGTGKTAGFTLPILQRLAGKPAAAPKTVRALILAPTRELAAQVEESVRIYGRYLKL
                     RSAVIFGGVGINPQIDLLKRGVDILVATPGRLLDHMQQGTIDLRRVEILVLDEADRML
                     DMGFIHDIRRVLAALPPQRQNLLFSATFSDEIKALADRLLNTPATIEVARRNSTVEII
                     EQKIHPVDRNRKHPLLAHLIKTNNWWQVLVFTRTKHGANKLVEQLEKDGIPAMAIHGN
                     KSQSARTRALSEFKDGKLQVLVATDIAARGIDIEELPHVVNYDLPNVPEDYVHRIGRT
                     GRAGAQGEAVSLVCVDEHDMLKGIEKLIKRQLPREVVPGFEPDPNATPQPIQLRSQPP
                     RRQPRAQGKAPAPATGARAGALPCALGW"
     gene            complement(1365..2159)
                     /locus_tag="VM406_12500"
     CDS             complement(1365..2159)
                     /locus_tag="VM406_12500"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HVL76829.1"
                     /translation="MHPMLNTAVKAARRAGAVINRASFDIDRVKVSEKGRNDFVTEVD
                     QAAENAIIEVLKNAYPDHAILAEESGASANHHDEHENIWIIDPIDGTTNFIHGFPTYC
                     VSIALKQRGQITQAVVYDPNRNDLFTATKGAGAYLNEKRIRVSRRDKMADALIGTGFP
                     FRSTDELDEYVKMFKILSERCAGLRRPGAAALDLAYVAAGRLDGFFEKGLQPWDVAAG
                     SLLITEAGGIVSNFAGESDYLYRGDVIAGTPKIFAQMVALLSRFAK"
     gene            2280..3053
                     /locus_tag="VM406_12505"
     CDS             2280..3053
                     /locus_tag="VM406_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HVL76830.1"
                     /translation="MNPSESPIPLFASTRFVLVETSHPGNVGSVARAMKTMGFSELVL
                     VNPRFADAPQQEEARAFASGALDVLDRARVVGSFDEAIEGCNFAAALSARLREFSPPV
                     FVPRELAAHVVSSDALRPALVFGSERYGLPNEIIERCNVLINIPANPAYSSLNLAQAA
                     QVLAYELRTAAMQGQGRPSNGIGFQGEPADLAQVEGMYAHLEQALVAIDFLDPANPRK
                     LMPRLKRLFSRAGLEKEEVNILRGIAHHILAQRQRKDGA"
     gene            complement(3054..3704)
                     /gene="lexA"
                     /locus_tag="VM406_12510"
     CDS             complement(3054..3704)
                     /gene="lexA"
                     /locus_tag="VM406_12510"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HVL76831.1"
                     /translation="MLKLTARQEQILNLIREAIENTGFPPTRAEIAAELGFRSANAAE
                     EHLQALARKGAIELSPGTSRGIRLREPRPERGASQMALPHPALMQLSLPLVGRVAAGS
                     PILAQEHIESTYQVDPTLFSAKPDYLLKVRGMSMRDAGILEGDLLAVKKADMAKNGQI
                     VVARLGDDVTVKRYRKSGALIELLPENPDFEPIRVEPGREDFALEGLAVGLLRSWA"
     gene            3912..4280
                     /gene="rpsF"
                     /locus_tag="VM406_12515"
     CDS             3912..4280
                     /gene="rpsF"
                     /locus_tag="VM406_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HVL76832.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMIERYKGIVTGRNGKVHRVEDWGR
                     RQMAYMIQKLAKAHYVCMNIECDGETLAEIETGFKFNDAVLRHLTVKMKKAETGPSPM
                     MKAVQKEDAAKAPRAEASAS"
     gene            4287..4634
                     /gene="priB"
                     /locus_tag="VM406_12520"
     CDS             4287..4634
                     /gene="priB"
                     /locus_tag="VM406_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HVL76833.1"
                     /translation="MRDEGSANLLRLIASIAERDVLRYTPAGIPIVNARLLHESEQVE
                     AGVVRKVEFEIAALAAGEVSGRFNRAPLGAVMAFTGFLARKGRNSKGLVFHVTNLETQ
                     PSDTDTGAQDGIR"
     gene            4621..>4860
                     /gene="rpsR"
                     /locus_tag="VM406_12525"
     CDS             4621..>4860
                     /gene="rpsR"
                     /locus_tag="VM406_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HVL76834.1"
                     /translation="MAFGKKFDKSKLKQRRQQQNPLFKRKKFCRFTAAGVEQVDYKDV
                     DTLKDFVQDNGKIMPARLTGTKAHYVCMNIECDGET"
BASE COUNT          886 a         1423 c         1619 g          932 t
ORIGIN      
        1 gccagccgag agcgcagggc aaagcgccgg cccgtgctcc cgtggcgggc gccggcgctt
       61 tgccctgcgc tctcggctgg cggcgcggcg gctggctgcg cagctggatc ggctgaggcg
      121 tggcgttcgg gtctggttcg aagccgggca cgacctcgcg cggcagctgg cgcttgatca
      181 gcttttcgat gcccttgagc atgtcgtgct cgtccacgca gaccagcgac acggcttcac
      241 cctgtgcccc ggcgcggccg gtgcggccga tgcgatggac atagtcctcg ggcacattcg
      301 gcaggtcgta gttgacgaca tgcggcagtt cttcgatgtc gatgccgcgc gccgcgatgt
      361 cggtggcgac cagtacctgc agcttgccgt ccttgaattc ggacagcgcg cgcgtgcgcg
      421 ccgactggct cttgttgcca tggatcgcca tcgccgggat gccgtccttc tcgagctgct
      481 cgaccagctt gtttgcgccg tgcttggtgc gcgtgaagac cagcacctgc caccagttgt
      541 tcgtcttgat caggtgcgcc agcagcgggt gcttgcggtt gcggtcgacc ggatggattt
      601 tctgctcgat gatctccacc gtcgaattgc gtcgcgcgac ttcgattgtc gcaggcgtgt
      661 tcagcaggcg gtcagcgagc gccttgattt cgtcggagaa ggttgccgag aacagcagat
      721 tctggcgttg cggcggcagc gcggcgagca cgcggcggat gtcatggatg aagcccatgt
      781 ccagcatgcg gtccgcttca tcgagtacga ggatttcgac gcggcgcaag tcgatcgtgc
      841 cttgttgcat atggtcgagc agtcgacccg gcgttgcaac caggatgtcg acaccgcgct
      901 tcaacaagtc gatctgcgga ttgatgccga caccgccgaa aatcaccgcc gagcgcagct
      961 tcaggtagcg gccgtagatg cgcactgatt cctcgacctg tgcggcaagc tcacgcgtgg
     1021 gggcgaggat cagcgcacgc accgtcttgg gcgcggcggc cggcttgcct gccagccgct
     1081 gcaggatcgg caatgtaaag ccggcggtct tgccggtgcc ggtctgggcg ccggcgagca
     1141 ggtcgccgcc cttcaggacg gccggtaccg cctgggcctg gatcggggtg ggagtgtggt
     1201 aaccctgttc ggttaccgcg cgtacgattt cttcggaaag tccgagttca gcaaaagaca
     1261 tgtatacctt caagaaaaaa acagggccgc tcgacggtgc gcgaagcagc ggcgagcggt
     1321 gacggcatgc agccgggctg catgtgcgat ggtggaattg gaatttactt cgcgaagcgc
     1381 gacagcagtg cgaccatctg cgcgaagatc ttcggcgtgc cggcaatgac gtcgccgcgg
     1441 tacaggtaat ccgattcgcc ggcgaagttg gagacgatgc caccggcctc ggtgatcagc
     1501 agcgacccgg ctgccacatc ccagggttgc aaacccttct cgaaaaagcc gtcgagccgg
     1561 ccggccgcga catacgcgag gtcgagcgcg gcggcgccgg ggcggcgcag gccggcgcag
     1621 cgttcgctca ggatcttgaa catcttcacg tactcgtcga gctcgtcggt gctgcgaaac
     1681 gggaagccgg tgccaatcag cgcatcggcc atcttgtcgc gccggctcac gcggatgcgc
     1741 ttctcgttca ggtaggcgcc ggcgcccttg gtagcggtga acaggtcgtt gcggttcggg
     1801 tcgtagacga cagcctgcgt gatctggccg cgctgtttca gcgcgatcga gacgcaatag
     1861 gtgggaaagc cgtggatgaa gttggtggtg ccgtcgatcg gatcaatgat ccagatgttc
     1921 tcgtgctcgt cgtgatggtt ggccgatgcg ccggattcct cggccaggat cgcgtgatcg
     1981 ggataggcgt tcttcagcac ctcgatgatg gcgttctccg ccgcctggtc gacttcggtg
     2041 acgaagtcat tgcggccttt ctcgctgacc ttgacgcggt cgatgtcgaa ggaggcgcgg
     2101 ttgatgacgg cgccggcgcg gcgggcagcc ttgaccgccg tattgagcat gggatgcata
     2161 aaggttccga tagaatggcg gcacccacgc caagcgcgtc ggaaccgcaa agaatccacg
     2221 ttaatgagcg atgcggcaag aaacccgaaa ggtgatgccg cgcaatcgcg ctattttaaa
     2281 tgaacccgtc cgaaagccct atccccctgt tcgcaagcac gcgattcgtt ctggtcgaga
     2341 ccagtcatcc cggtaatgtc ggctccgtcg cccgtgcgat gaagacgatg ggtttctcgg
     2401 aactggtgct ggtgaacccg cgttttgcgg atgcgccgca gcaggaggag gcgcgcgcgt
     2461 tcgcaagcgg tgcgctggac gtgctcgacc gcgcgcgtgt cgtcggaagc ttcgacgagg
     2521 caatcgaggg atgcaatttt gccgccgcgc tgagcgcgcg cctgcgcgag ttctcgcccc
     2581 cggtgtttgt gccgcgcgag cttgcggcgc acgtcgtgag ctcggatgca ctccggccgg
     2641 cgctggtgtt cgggagcgag cgctatggct tgccaaacga gatcatcgag cgctgcaacg
     2701 tattgatcaa tattcccgcc aatccggcgt attcgtcgct gaatctggcc caggctgcgc
     2761 aagtgctggc ctacgagttg cgcacggcgg cgatgcaagg gcagggaagg ccgagcaacg
     2821 gtatcggatt tcagggggaa cccgctgatc tcgctcaggt ggagggcatg tacgcgcacc
     2881 tcgagcaggc actggttgcg atcgatttcc tcgacccggc caatccccgc aagctgatgc
     2941 cgcgcttgaa gcgcttgttt tcacgcgccg ggctggagaa ggaggaagtc aatatcctgc
     3001 gcggcatcgc gcatcacatc cttgcgcagc ggcagcgcaa ggacggcgcc tgatcaggcc
     3061 cagctgcgca gcaggccgac tgccagtcct tccagtgcga agtcctcgcg tcccggctcg
     3121 acccggatcg gttcgaaatc cggattttcg ggcagcaact cgatcagggc gccggatttc
     3181 cggtagcgct tgactgtcac gtcatctccc agtcgggcaa cgacgatctg gccattcttg
     3241 gccatgtcgg ccttcttcac cgcgagtagg tcgccttcga ggatgccggc gtcgcgcatc
     3301 gacatcccgc gcaccttcag caggtagtcg ggctttgcgg aaaacagtgt cgggtctacc
     3361 tggtaggtcg attcgatatg ttcctgggca agaatcggcg agccggctgc cacccgtcca
     3421 accaacggca ggctcagctg catcagcgcc ggatgcggca gggccatctg gctcgcgccg
     3481 cgttcggggc gcggttcgcg caggcgtatg ccgcgggacg tgccgggcga gagctcgatc
     3541 gcgcctttgc gggcgagggc ctgcaggtgc tcctcggcgg cattggccga gcgaaagccg
     3601 agttcggctg cgatttcggc gcgcgtcgga ggaaagccgg tgttttcgat cgcttcccgt
     3661 atgaggttga ggatctgttc ctggcgagcg gtgagcttga gcatgggaag cggggcaggg
     3721 ctgagggtat ggacagcctg tatttttata cagccatcgc cgggatgcaa ggaattttcc
     3781 cggtttcttg cgcaagcgct tgacgcgatt gaatttttgc cgcatgcacg ttatgatggc
     3841 gggctttcct cttcccacac tcgtgttgac gccggggtgg gcgaaacgtc aatccgtcca
     3901 aaggagatta catgcgtcat tatgaaatcg tttttatcgt ccatccggat cagagcgagc
     3961 aggtacccgc gatgatcgag cgctacaagg gcatcgtcac cggccgcaac ggcaaggtgc
     4021 atcgtgtcga agactggggc cgtcgccaga tggcttacat gatccagaag ctggccaagg
     4081 cgcactatgt gtgcatgaac atcgagtgcg atggcgaaac gctggccgag atcgaaaccg
     4141 gcttcaagtt caatgacgcc gtgctgcgcc acctgacggt gaagatgaag aaagccgaga
     4201 ccggtccgtc gccgatgatg aaggccgtgc agaaggaaga cgctgccaag gccccgcgcg
     4261 ccgaggcgtc cgcttcgtaa tttcttgtgc gtgacgaggg cagcgcgaat ctgctgcgcc
     4321 tgattgccag catcgccgag cgggacgtac tccgttatac gccggccggc atcccgatcg
     4381 tgaatgcaag attgcttcat gaatcggaac aggtggaagc gggcgtggtg cgcaaggttg
     4441 aattcgagat cgccgcgctg gccgcgggag aagtttcggg aagattcaat cgggcgccgc
     4501 tgggagcagt gatggcattt accggcttcc tggccaggaa aggccgcaat agcaaaggcc
     4561 tcgtgttcca cgtcacgaat ctagaaaccc aaccatcaga tacagataca ggagcccaag
     4621 atggcattcg gtaagaaatt tgacaaaagc aagctcaagc aacgtcgcca gcagcagaac
     4681 ccgctgttca agcgcaagaa gttctgccgc ttcaccgccg ccggcgtcga gcaggtcgac
     4741 tacaaggatg tcgacaccct gaaggacttc gtccaggata acggcaagat catgccggcc
     4801 cgcctgaccg gcaccaaggc gcactatgtg tgcatgaaca tcgagtgcga tggcgaaacg
//
LOCUS       DATLGF010000408         9846 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_62920,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000408 DATLGF010000000
VERSION     DATLGF010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 9846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9846
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62920"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VM406_12530"
     CDS             complement(<1..615)
                     /locus_tag="VM406_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HVL76835.1"
                     /translation="MSEQIDKLVPPGEGLVSRSIFVDDEIFRQEMRQIFSRAWLFVGH
                     ESLIPKPDDYFSSRMGTDPVILTRDRNGEIHVFLNSCTHRGMKLCRHDQGNSRNFTCP
                     YHGWSFSTDGKLADRPGGLAGVPGYATHYKGELKKEDWGLKRVAKVTNYKGTIWATWD
                     KDAPDFLDYLGDMRHYLDAALDCRDGRPGGSELIGGVQKWRVKCN"
     gene            805..1347
                     /locus_tag="VM406_12535"
     CDS             805..1347
                     /locus_tag="VM406_12535"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HVL76836.1"
                     /translation="MNDISFPPADVDYSIIISPRPGRCDGAIRAFPFYFPLRFPMTLS
                     RLPSPSASPVSRAVRITAPQEIVAFSAQFPAVVNEQADPQSVAAFGDTATQTRTTLER
                     MAAVLAEVDMTLANLVSMRVYLVADPALGKMDFAGFNEACKAFFKTKDESQFPARTVL
                     QAAGLVNPGWLIEIEVVAAR"
     gene            1383..1751
                     /locus_tag="VM406_12540"
     CDS             1383..1751
                     /locus_tag="VM406_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVL76837.1"
                     /translation="MIDIIPFDAASVAPVDDPVVPERHVSGHPKRTAWPQYANASGEF
                     KCGIWESAKGAWRTVFHQSKDEYFVVLEGRVRLCAEGGEPRELGPGDACVIPAGFVGI
                     FEVVEPVRKHYVIFERAARA"
     gene            complement(1917..1993)
                     /locus_tag="VM406_12545"
     tRNA            complement(1917..1993)
                     /locus_tag="VM406_12545"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1957..1959),aa:Arg,seq:acg)
     gene            complement(2154..3368)
                     /locus_tag="VM406_12550"
     CDS             complement(2154..3368)
                     /locus_tag="VM406_12550"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HVL76838.1"
                     /translation="MSGQAILAVNAGSSSLKFALYPCSGSEIGPARLSGTVEGLEPGG
                     RPCMRIKGDTVELRQPIVVAGDGAPFDAALKAVQAALEHHAAGMRMAAVAHRIVHGGE
                     RLLRSVRLDEEALHYLDSRAPLAPLHQPHNLAGVRAFAGRYPDLPQIGCFDTAFHASL
                     PQLETLYALPRALREQGIRRYGFHGLSYQYVCAELKAHSGRADGRMVLAHLGNGASAC
                     AVRDGKSVATTMGFSALDGLVMGTRSGSLDPGVLLYLMQRGWSAERLEQLLYRECGLR
                     GVSGISADMRTLRASEAPEAKLAIDLFIHRLVRECGALAACLQGLDLIAFTGGIGEHD
                     AALRARTCDALAWLGVRIDRAANAAADGSAAAPVHAAGSGVEIWVVPTDEGRVAAAEA
                     ARLIALASGARG"
     gene            complement(3365..4771)
                     /locus_tag="VM406_12555"
     CDS             complement(3365..4771)
                     /locus_tag="VM406_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HVL76839.1"
                     /translation="MTDPVPQQIENVTYDELRVGQSARLVRTLALSDIHAFAAVSGDY
                     NPAHLDADYALNTLFHGVVAHGMWGGTLISALLGTVFPGPGTVYLEQSLRFVRPVRVG
                     DTLTVTATVTSKDDPTRRVGLDCQVVNQDAVVVLEGKALVLAPTQKVCRPVVKLPPVR
                     MFDPEARLEALLALVDRLEPVRCAVVHPCDPESLRGAMDAAARKLIVPVLVGPAAKIR
                     TAAQAAGVALEGIELHDVAHSNAAADTAAGLAAAGEVEALMKGSLHTDELMQAVLANR
                     TLRTKRRLSHVFRFDIPMYDKPLLISDAALNIRPTLEEKADIVQNAITLCHALGIANP
                     KIAILSAVETINTKLQSTLDAAALCKMADRGQITGGTLDGPLAFDNAVSMEAVRIKGI
                     TSPVAGNADVLIVPDLESGNMLAKQLEYLAGASGCGVVVGARVPIALTSRADGPRARV
                     ASAALALLLAHRNREIKP"
     gene            complement(4839..7544)
                     /locus_tag="VM406_12560"
     CDS             complement(4839..7544)
                     /locus_tag="VM406_12560"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVL76840.1"
                     /translation="MTSLRKLHNHHLLLIMFLVGAASGFLVSSAGWWGLNRGAHDLAA
                     LQASTGNPLRLAGETQAMALHVQSLYLDALRKRDLPEAEMAALAHAETELFRVIQEGV
                     QTDAARAVEWGNIAAAHARLEKSRRHLLALVGQEPERALHYHAGTTQPLADALLAETR
                     ALLPAVLAENAAVQLRAEADNARARALLIALSVLASVFFCATAALTWRESAARRRNEL
                     AREREHTMFRTLFQGSKDGVILLRNARVADMNRSARALFALPDGADAQALDLDRLCAD
                     SGTGSLQERIAHGRPDQARRFEWEFQALDGRRFPAEVAVDIANLGKREVVQVTVRDIT
                     RRKEIERSMRLTNQAFENSVEGIAITDANGNILTVNETFTTITGYARDEVIGRNPRIL
                     SSGRQTREFYQAMWETLSIAGKWQGEVWNKRKNGDIYPEWLTISRVVDEHGRVTNFVG
                     VFSDITERKSAEERLLYQVYYDQLTDLPNRVLFNDRLGQVLSMARRHDDRPFAVMFLD
                     LDRFKTINDTMGHQVGDRLLQQAAHRLRGAVRESDTVARMSGDEFTVLLSEIGNERHA
                     ANVAQKILEAFRQPFQLEGQDVYVAASIGIAVYPTDGRTPEALLKHADMAMYRAKGAG
                     GSWYELYDAGMASVANERFVLETALHKALERNELELHYQPQFECESGRLAGFEALLRW
                     GHPELGMVSPAQFIPLAEETGMIVDIGEWVLRSACAQAKSWERTGHPLRVAVNLSARQ
                     FRQGDLADVVQATLAEFRLAPQLLELELTESILMEDSADTGATLEKLHAMGVQISIDD
                     FGTGYSSLSYLKRMPIQVLKIDRSFVRDIHQDPDDRAIVRAVIALAHNMKLKVVAEGV
                     ETPEQLAFLREHGCDTVQGYLFSRPIPGGELLALLAAGAALGSPA"
     gene            7827..8555
                     /locus_tag="VM406_12565"
     CDS             7827..8555
                     /locus_tag="VM406_12565"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HVL76841.1"
                     /translation="MSKMTLDRILQSQGFGTRKWCRQLVADGEVTVNGELVDDFRKSF
                     ETDGLRFTVFGEAWLYRDHVYLALHKPSGYECSRKPSHHPGVLSLLPEQFSWRAVQPV
                     GRLDHDTTGLLLLSDDGAFIHAQTSPRRHVPKHYLARTAEPVDETLIARLKEGVQLHD
                     EPAPLAAQDCRILSEQEIEVVLEQGKYHQVKRMLAAAGNHCVQLHRTAIGGLRLADLE
                     LEPGQWCYLDAAELDMLSSTATSG"
     gene            8653..>9846
                     /locus_tag="VM406_12570"
     CDS             8653..>9846
                     /locus_tag="VM406_12570"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HVL76842.1"
                     /translation="MPDTEYRRLIQAAKDKALFGFLAVVQRTMQDADRNVADQLAEAR
                     SGLDRSALTAVRHFLRQDGSTFLRNIDGMYRAALERAMQTMYIDLRPGMRKLLASELS
                     LIDDEAVNQQIEVGRLAQRMREAHEEGIGRLNVIIANLHGQVEARERENPFRPYLLAR
                     SLYEAVRQSVDEEGKDKLLYEQLGDAMLPHLAGFYGAIREVFEAAGVRGRFIAQRSRA
                     AHNQRYFGAPGAPGTAAAGEMMPGLGRMIEAMSRAAGHAPAAEPPSAHALVRSMFTSP
                     REPSSRADAPSLLVEQLGGLQEQATHAPPASPTGAPGRAQVEALRERLDLDAATDDER
                     LTVEVVTMLFEIMLEDERVPAVLRDQIARLQVPTLKAALLEPSLLQDEGHPVRRLLNR
                     LSIAAA"
BASE COUNT         1591 a         3254 c         3271 g         1730 t
ORIGIN      
        1 gttgcacttc acgcgccact tctgcacgcc gcctatcagt tccgacccgc cgggacggcc
       61 gtcgcggcag tccagcgcgg cgtcgaggta gtggcgcatg tcgccgaggt agtcgaggaa
      121 atccggcgca tccttgtccc aggtcgccca gatcgtgccc ttgtagttgg tgaccttggc
      181 gacacgcttc agcccccagt cttccttctt caactcgccc ttgtagtgcg tggcgtaacc
      241 gggcacgccg gcgagcccgc ccggacggtc ggccagcttg ccgtcggtgg agaagctcca
      301 gccgtgatag gggcaggtga agttgcggct gttgccctgg tcgtggcggc acagcttcat
      361 gccgcgatgg gtgcagctgt tcaggaagac atggatctcg ccgttgcggt cgcgcgtgag
      421 gatgaccgga tcggtgccca tgcgcgagga gaagtagtcg tccggcttgg gaatcaggct
      481 ttcatgaccg acgaacagcc aggcgcggga aaagatctgc cgcatctcct ggcggaagat
      541 ctcgtcatca acgaagatcg atcggctcac cagcccttca cccggcggca ccagcttatc
      601 gatttgttcg gacatcgctc tctccttcgt ttcgttggtc tgcatcgtct tgccgtttcc
      661 ggtatggtgt ccgttccggc ttcgcgtcgg ctccggggcg gccatcgcac cgcaccccgg
      721 gcccatgctt cggtctgctg caacggcgga ctttatcgta tgaaagtcgg ataatactac
      781 aaacgcaggt gtgatcaagc ggaaatgaac gatatttcat tcccgcctgc ggatgttgat
      841 tatagtatta tcatatcgcc tcggccgggc cgctgcgacg gtgccatccg ggccttcccc
      901 ttttactttc ctctcaggtt ccccatgacg ctttcccgcc ttccttctcc gtccgcttcc
      961 ccggtttcgc gtgccgtacg catcacggcc ccgcaggaaa tcgtcgcgtt cagcgcgcag
     1021 ttcccggccg tggtcaacga acaggccgac ccgcaatcgg tggcggcctt cggcgatacc
     1081 gcgacgcaga ccaggaccac gctggagcgt atggcggccg tgctggcgga agtggatatg
     1141 acgctggcga acctggtttc gatgcgtgtc tatctggtcg ccgatcctgc tctgggcaag
     1201 atggatttcg ccggtttcaa cgaagcgtgc aaggcatttt tcaagaccaa agacgagagc
     1261 caattcccgg cgcgcaccgt gctgcaggcc gcggggctgg tcaatccggg atggctgatc
     1321 gagatcgaag tcgtcgctgc gcgctgagca gttctttatt cccgacattt caaggagcac
     1381 acatgatcga catcattccc tttgacgccg catccgtcgc accggtggac gacccggtgg
     1441 taccggagcg ccatgtatcc ggccatccga agcgcaccgc ctggccgcaa tacgcgaacg
     1501 cgtcaggtga attcaagtgc gggatctggg agagcgcgaa gggggcgtgg cgcacggtgt
     1561 ttcaccagtc gaaggatgaa tactttgtcg tgctggaggg gcgggtgcgc ctttgcgccg
     1621 agggcggcga gccgcgcgag ttgggcccgg gcgatgcctg cgtgattccg gcgggcttcg
     1681 tcggcatctt cgaggtggtc gagccggtgc gcaagcacta tgtgattttc gaacgcgccg
     1741 cacgggcttg acgggaggac cgggcttgca ggtgcgagcc cggtcgccga gctgtgccag
     1801 gatggggcgg ggcggtgcgc aggtgtgatg gggaaggcga ccttacagcg gaggccgagc
     1861 ggacgccgta aaccagaaaa ggggttagat cttgcgatct aacccctcgg taaaactggc
     1921 gcggccggca ggaatcgaac ccacgacccc ttggttcgta gccaagtact ctatccagct
     1981 gagctacggc cgcgaaagac gaaattatag ccgggctatt tcagaaacgg aagcccctgc
     2041 gcaaaaattt ccaaccggca tgcaaggggc cggcattgtt caatgcgcaa acggccaggc
     2101 tgccctcact gcggtttacc tgcgcacccg ggtcgttttc ggtgacatcc gcttcatccg
     2161 cgtgcgccgg aagcaagggc gatgagccgc gccgcttctg ccgccgccac ccgtccctcg
     2221 tctgtgggca ccacccagat ctcgactccg ctcccggccg catgcacggg cgcggcggcg
     2281 ctgccgtcgg ccgctgcatt ggccgcccga tcgatgcgca cgcccagcca ggcaagcgca
     2341 tcacaggttc gcgcgcgcag cgccgcgtca tgttcgccga tgccgccggt gaacgcgatc
     2401 aggtcgagtc cttgcaggca ggcggccagc gcgccgcatt cgcgcaccag cctgtggatg
     2461 aacagatcga tcgccagttt cgcctccggc gcctcgcttg cgcgcagcgt gcgcatgtcg
     2521 gcggagatgc ccgacacgcc gcgcagtccg cattcgcgat acagcagctg ttccagccgc
     2581 tcggcgctcc agccgcgctg catcaggtac agcagcacgc cgggatcgag cgatccgctg
     2641 cgcgtgccca tcaccagtcc gtccagtgcg gaaaatccca tcgtggtcgc cacgcttttc
     2701 ccatcccgta ccgcgcaggc gcttgcgccg ttgccgaggt gggcgagcac catgcggccg
     2761 tcggcgcggc cgctgtgcgc cttcagctcg gcacacacgt attggtagga caggccgtga
     2821 aagccgtagc gccgtatgcc ttgctcgcgc agcgcgcgcg gcagcgcgta cagggtttcc
     2881 agctgcggca ggctggcgtg gaacgcggta tcgaagcagc cgatctgcgg cagatccgga
     2941 tagcggccgg cgaacgcgcg cacgccggcc aggttgtgcg gctggtgcag cggagcgagc
     3001 ggcgcccggc tgtccaggta atgcagcgct tcctcgtcca ggcggacgga acgaagcagc
     3061 cgctctccgc catgcacgat gcgatgcgcg acggccgcca tccgcatgcc ggcggcatgg
     3121 tgttcgagcg cggcctgcac ggccttgagc gcggcgtcaa acggcgcgcc atcgccggcc
     3181 accacgatcg gctggcgaag ctcgaccgtg tcgcccttga tgcgcatgca gggccggccg
     3241 cccggctcca ggccttccac ggtgccgctt aggcgcgccg gcccgatttc cgatccgctg
     3301 caagggtaga gcgcgaactt cagcgatgac gagccggcgt tcacggcaag gatggcctga
     3361 ccgctcacgg cttgatctcc cggttgcggt gggcgagcag cagcgccagc gccgccgagg
     3421 cgacgcgggc gcgcggtccg tcggcacggc tggtcagcgc gatcggcacg cgcgcgccga
     3481 cgaccacgcc gcatccgctc gcaccggcca ggtattcgag ctgcttggcg agcatgttgc
     3541 ccgactcgag atccggcacg atcagcacgt cggcattgcc ggccaccggc gaggtgatgc
     3601 ccttgatgcg caccgcttcc atcgagaccg cgttgtcgaa tgcgagcggc ccgtccaagg
     3661 tgccgcctgt gatctggccg cggtcggcca tcttgcacag cgcggcggcg tccagcgtcg
     3721 actgcagttt cgtattgata gtttccaccg cggacaggat cgcgatcttc ggattggcaa
     3781 tgcccagcgc gtggcacagc gtgatcgcgt tttgcacgat gtcggccttc tcttcgagcg
     3841 tcggccggat gttgagcgcg gcgtccgaaa tcagcaaggg cttgtcgtac atcggaatgt
     3901 cgaagcggaa cacatgcgac agccggcgct tggtgcgcag cgtccggttc gcaagcacgg
     3961 cctgcatcag ttcgtcagtg tgcaggctgc ctttcatcag cgcctcgacc tcgccggcgg
     4021 ccgcgagccc ggctgcggtg tcggcggcgg cattgctgtg cgcaacgtca tgcagctcga
     4081 tgccttcgag tgcaacgcca gcggcctgcg cggccgtccg gattttcgcc gcggggccga
     4141 ccagcaccgg aacaatcagc ttgcgcgcgg ccgcatccat tgcgccacgc agcgattccg
     4201 gatcgcaggg atgcacgacc gcgcagcgca ccggctccag ccggtcgacg agggccagca
     4261 gcgcctccag gcgcgcctcc ggatcgaaca tgcgcaccgg cgggagcttg accaccggcc
     4321 ggcacacctt ctgggtcggc gccagcacca gtgccttgcc ctcaaggacc acgaccgcat
     4381 cctggttcac cacctggcaa tcgagaccga cgcgcctggt cgggtcgtcc ttcgatgtga
     4441 cggttgcggt gacggtcagc gtgtcgccga cacgcaccgg tcgcacgaag cgcagcgact
     4501 gttccaggta caccgtgccc gggccgggaa ataccgtgcc gaggagggcg gagatcaggg
     4561 ttccgcccca catgccgtgc gccaccacac cgtggaacag ggtgttgagc gcgtagtcgg
     4621 cgtcgaggtg ggccgggttg tagtctcccg agaccgccgc gaacgcgtgg atgtcggaca
     4681 gcgcgagtgt gcgcaccagc cgggcggact ggccgacgcg caactcgtca taggtgacgt
     4741 tctcgatctg ctggggaacg ggatctgtca tcgtgtttcg ggtcgttacg aaactgaatt
     4801 ggaagcaagc aagaggcatg cccctgcccg cgctgggatc aggccggact gccaagggct
     4861 gcgccagccg cgagcagcgc caggagctcg ccgccgggaa tcggcctgct gaacaggtaa
     4921 ccctgcacgg tgtcgcagcc gtgttcacgc aggaaggcca gttgctcggg cgtttccacg
     4981 ccttcggcga ccaccttgag cttcatgttg tgcgcaagcg cgatcacggc gcggacgatc
     5041 gcgcggtcgt cgggatcctg gtggatgtcg cgcacgaacg agcggtcgat cttcaggacc
     5101 tggatcggca tgcgcttcag gtaggacagc gacgaatagc cggtgccgaa gtcatcgatc
     5161 gagatctgca cccccattgc atgcagtttc tccagcgtcg ctccggtgtc cgcggagtcc
     5221 tccatcagga tgctctcggt cagttcgagt tcgagcagct gcggcgccag ccggaactcg
     5281 gccagcgtgg cctgcacgac gtcggccagg tcaccctggc ggaactggcg cgccgacagg
     5341 ttgacggcca cgcgcagcgg atggccggtg cgttcccagg acttcgcctg cgcgcaggcg
     5401 ctgcgcagca cccactcgcc aatgtccacg atcatgccgg tttcctcggc cagcggaatg
     5461 aactgcgcgg gcgagaccat gccgagctcc ggatggcccc agcgcagcag cgcttcgaat
     5521 ccggcaagac gcccgctttc gcactcgaac tgcggctgat agtgcagctc cagctcgttg
     5581 cgttcgagcg ccttgtgcag cgcggtctcg agcacgaagc gttcgttcgc cacgctggcc
     5641 atgccggcgt cgtacagctc gtaccaggag ccgcccgcgc ccttggcgcg gtacatcgcc
     5701 atgtcggcgt gcttgagcag cgcctccggc gtgcgcccgt cggtcgggta cacggcaatg
     5761 ccgatgctcg ccgcgacata gacgtcctgt ccttcaagct ggaacggctg gcgaaacgcc
     5821 tccaggatct tctgggccac gttggctgcg tggcgctcgt tgccgatctc gctcaacagg
     5881 accgtgaatt cgtcgccgct catgcgtgcg acggtgtccg attcgcgcac cgcgccgcgc
     5941 aggcggtgcg cggcctgctg cagcagacgg tcgccgacct ggtgccccat cgtgtcattg
     6001 atcgtcttga agcggtccag gtcgaggaac atcacggcga acggccggtc gtcgtggcgc
     6061 ctggccatcg atagcacctg gccgagccgg tcgttgaaca gcacccggtt cggcaggtcg
     6121 gtcagctggt cgtagtagac ctgatagagc agccgctctt cggcggactt gcgctctgtg
     6181 atgtcggaga acacgccgac gaagttggtg accctgccgt gctcgtcgac gacccggctg
     6241 atggtcagcc attccgggta gatgtcgccg ttcttgcgct tgttccagac ctcgccctgc
     6301 cacttgccgg cgattgacag cgtctcccac atcgcctggt agaactcgcg cgtctgccgg
     6361 ccggagctga ggatgcgcgg attcctgccg atgacttcgt cgcgcgcata gccggtgatc
     6421 gtggtgaagg tctcgttcac ggtcagaatg ttgccgtttg catcggtgat cgcgatgcct
     6481 tcgaccgaat tttcgaaggc ctggttggtc aggcgcatgc tgcgttcgat ttccttgcgc
     6541 cgtgtgatgt cgcgcacggt cacctgcacg acctcccgct tgccgagatt ggcgatgtcg
     6601 accgcgactt ccgccgggaa gcggcgcccg tcgagcgcct ggaactccca ttcgaagcgc
     6661 cgcgcctggt ccgggcggcc gtgcgcgatg cgttcctgca ggcttccggt tccggagtcg
     6721 gcgcacagcc ggtcgaggtc gagcgcctgc gcgtcggcgc cgtccggcag tgcgaagagt
     6781 gccctggcgg accggttcat gtccgccacg cgcgcgttcc gcagcaggat cacgccgtct
     6841 ttgctgccct ggaacagggt gcggaacatc gtgtgctcgc gttcgcgcgc caactcgttg
     6901 cggcgacgcg cggcggattc gcgccaggtc agcgccgcgg tggcgcagaa aaacacgctc
     6961 gccagcaccg acagcgcaat caagagtgca cgcgcacggg cgttgtccgc ttccgcgcgc
     7021 agctgtacgg cggcattctc ggcgaggacg gcgggaagca gcgcgcgcgt ctcggcaagc
     7081 aaggcgtcgg ccagcggctg ggtggtgccg gcgtggtagt gcaacgcccg ctccggctcc
     7141 tgaccgacga gcgcgagcag gtgacgccgg ctcttttcca ggcgtgcatg cgcggcggcg
     7201 atgttccccc attcgacggc gcgcgccgcg tcggtctgca ccccttcctg aatcacccgg
     7261 aaaagctcgg tttccgcgtg cgccagcgcc gccatttcgg cctccggcag gtcccgcttg
     7321 cgcagcgcgt cgaggtacag gctttggacg tgcagtgcca ttgcctgggt ctcgccggcc
     7381 aggcgcaacg gattgccggt gctggcctgc aaggccgcca ggtcgtgggc gccgcggttc
     7441 agcccccacc agccggcgct gctgacaagg aagccggacg cagcccccac gaggaacatg
     7501 atcagcaaca gatggtggtt gtgcagcttg cgcagggagg tcatcggatg gcgttaggaa
     7561 ggccgcgagc gggcagggtg cccgcgaggt tgttacacct gagcaaatgt tgtgccaagg
     7621 aaagaaggcc cgtaatcggc gctgatgcgg gcgcggccgc ttgacggtgc cgccgcgatt
     7681 cccgaaaacg ggaagcttcg gcatcccgct ctctcatcgc cgggaaaatc gccggccgac
     7741 acccgatccc tggattgcgc gcggagcgtc aattgacgag gctgcgagcg aaggctgcgc
     7801 gggcccgtct ctgctaaagt cgcgcgatga gcaaaatgac cctggaccgc atcctgcaat
     7861 cgcagggttt cggcacgcgc aaatggtgcc gccaactcgt cgccgacggc gaggtgactg
     7921 tcaacggcga gctggtcgac gatttccgca agagtttcga aacggacggg ctgcgtttca
     7981 ccgtgttcgg cgaggcttgg ctgtaccgtg accatgtcta cctggccctg cacaagccgt
     8041 cgggctacga gtgttcgcgc aagccgagcc accatcccgg cgtgctctcg ctgctccccg
     8101 aacagttttc ctggcgcgcc gtgcagccgg tcgggcggct cgaccatgac accaccggtc
     8161 tgttgctgct gtccgacgac ggcgccttca tccatgccca gacctcaccg cgccggcatg
     8221 tgcccaagca ctacctggcc cgcaccgccg aaccggtgga tgagacactg atcgcccggc
     8281 tgaaggaagg ggtgcagctg catgacgagc ccgcgccgct ggctgcgcag gactgccgca
     8341 tattgagtga gcaggagatc gaagtagtgc tggagcaagg caagtatcac caggtcaagc
     8401 gcatgctggc cgcggccggc aaccactgcg tccagttgca ccgcaccgcc atcggcggcc
     8461 tgcgcctggc ggacctggaa ctggagccgg gccagtggtg ctacctcgac gccgccgaac
     8521 ttgacatgct ttcctctacg gcaacgtccg gctgactcgc cgcggtttct tgttgcgtat
     8581 caacaatttt tgccggttgc cgcgcggatt ggccgtaaca ttgagttttg caaaatttcg
     8641 ttccgtacag gcatgcccga taccgagtac cgaagactga tccaggcggc gaaggacaag
     8701 gccctgttcg gcttcctggc cgtcgtgcaa cgcacgatgc aggatgccga ccgcaacgtc
     8761 gccgaccagc tcgccgaagc gagatccggc ctcgaccgca gcgcgctgac cgcggtgcgc
     8821 catttcctgc ggcaggacgg cagcaccttc cttcgcaaca tcgacggcat gtatcgcgcc
     8881 gcgctcgaac gggcgatgca gacgatgtac atcgacctgc gtcccgggat gcgcaagctg
     8941 ctggcgagcg agctttccct gatcgacgac gaggctgtga accagcagat cgaagtcggc
     9001 cggctggcgc agcgcatgcg cgaggctcac gaggaaggca taggccggct caacgtcatc
     9061 atcgccaatc tccacggaca ggtcgaggcg cgcgagcgcg agaacccgtt ccggccctat
     9121 ctgctggcac gctcgctgta cgaggcggtt cgccagagcg tggacgagga gggcaaggac
     9181 aagctcctgt acgaacagct cggcgatgcg atgctccctc acctggccgg cttctatggc
     9241 gcgatccgcg aggtgttcga ggccgccggt gtgcgcggcc gcttcatcgc gcagcgctcg
     9301 cgcgccgcgc acaaccagcg ttatttcgga gcgcccggag cgccgggtac cgccgctgcc
     9361 ggcgaaatga tgcccggcct cggtcgcatg atcgaggcca tgagccgggc tgccggccat
     9421 gcaccggccg ctgagccgcc ttccgcgcat gcactggtgc gctcgatgtt cacttctccg
     9481 cgcgagccgt cctcgcgcgc cgacgccccg agcctcctgg tggagcagtt gggcggcctg
     9541 caggagcagg ccacgcatgc gccgcccgct tcaccgaccg gtgctcccgg ccgcgcccag
     9601 gtcgaggcgc tgcgcgagcg actcgatctc gatgccgcca ccgatgacga gcgtttgacg
     9661 gtcgaagtgg tcacgatgct gttcgagatc atgctggagg acgagcgggt gccggctgtc
     9721 ctgcgcgatc agatcgcgcg cttgcaggtg cctaccctca aggcagcctt gctggagcct
     9781 tcgctgctgc aggacgaagg gcatccggtg cgacgcctgc tcaatcgcct gagcatcgcc
     9841 gcggcc
//
LOCUS       DATLGF010000409         5994 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_232423, whole genome shotgun sequence.
ACCESSION   DATLGF010000409 DATLGF010000000
VERSION     DATLGF010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232423"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="VM406_12575"
     CDS             <1..1209
                     /locus_tag="VM406_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HVL76843.1"
                     /translation="MQALAAAGVAPAVPVVALDDSEGGTAHSAWLAPHGQRPASVAIA
                     PDWPFNIIYSSGTTGTPKGIVQPHAMRWAHVLRGRMNGYDDTAVTLISTPLYSNTTLV
                     SFLPAIALGGSVVLMPKFDAAGWLALAEKHRATHAMLVPVQYQRIMAREDFERYDLSS
                     FRMKFCTSAPFPAALKADVLRRWPGGLMEYYGMTEGGGTCILAAHEHPDKLHTVGKPS
                     PGHDIRVIDDAGNEVARGEAGEVVGHSPGMMVGYHKQPEKTREAEWYAPDGKRFIRTG
                     DIGRFDEDGFLVLMDRKKDMIISGGFNIYPSDLEAVLLRHPSILEAAVIGVPSARWGE
                     TPVAAVVARNGTSLEPEQVLEWANAQLGKTQRLAAVSVTDRLPRSAIGKVLKRELREA
                     LAQRGALQVP"
     gene            complement(1216..2676)
                     /locus_tag="VM406_12580"
     CDS             complement(1216..2676)
                     /locus_tag="VM406_12580"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HVL76844.1"
                     /translation="MTPSADAQAKPSSAARIGLWLGPLWLLAVMILPAPGGMSEDAWR
                     CTGLALWMATWWATEAIPIPATSLLPIVLVPALGLGKLDATTASYANPIIFLFLGGFL
                     IGIAMQRWNLHRRIALNVLLLVGQRPERQIAGFMLATGFLSMWVSNTATTVMMLPIGI
                     SVVGLLGTDQDRAEVDRYAAALLLAIAYAASIGGIATLIGTPPNALLAAYLAKNHDIQ
                     VGFAQWMVIGLPVSVLMLLVAWWWLTRGGFRIKSGEGGRQALTNELAAQGRISRAEQR
                     VAAVFVVTAAAWILSPVITRYGVGWLSDTIIAIAAGITLFLLPSADKAQARLLVWEDA
                     KALPWGVLLLFGGGLALADVIRNSGLASWIAGQLAVFSALPLLLLAGAVVLVIVFLTE
                     LTSNTATAAAFLPLLGALAMSTGVDPLLLTVPAAIAASCAFMMPVATPPNAIVFATGQ
                     LKIQTMMRAGLFLNIAATVILTLVVTGLLQAFWLTD"
     gene            3425..5455
                     /locus_tag="VM406_12585"
     CDS             3425..5455
                     /locus_tag="VM406_12585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL76845.1"
                     /translation="MKTVTFEAERKSTSIPSALSTFHRIGKAELKTMESRWRAVEART
                     GGPIEQFDWAVACAMHTDASEVEVVGVMRGEELRAVVPLAIKRIAGVKRRMMLGVDEY
                     FEPMDFLFSDAQSLQRLMQQLARDRRPMLLSRVPTDSGSLDALRQAFRKRALVVERPG
                     EGSPFIPLDPSWSEPEQHLNAGRRSDLRRMRRRLDSMGKAESAIHAPTPDQLDALLEE
                     AFQVEARSWKGAAGTALLYDAKRRNHLRSYLKAAARSGEVRIGLLRLDGRAIAMEIAI
                     QRADALWLLKIGYDAAFAHCAPGQLLLRDTIEHAARTGLKNFEFLGVSAPWIDVWSLH
                     KHDCVAVWIYPPTVQGMLGFMADSGLRLARMLRRLAISGRRRLIKSIVPVLNFAAKSY
                     IAGETLADAMRVAEKVRANGASVTIGYWNTEDEKPAELAEQDVAGLDQLAHSGSDDYL
                     SVKLPALHFKKELVSKLSGAAAKAGRRIHFDSHGIEYVVRKMAVCEELKRLHPDLDIG
                     FTLPGRWARSVDDADWVCRQGLFVRVVKGQWPDPAQVNADPEQGFMNVIERLAGRARK
                     VAVATHDPRLAERALRRLQAAGTPCEMELLYGLPLRRCMQVARERGVPMRIYIPYGEA
                     YLPYALSQLRDNPRILWWLLRDSVAALRPGVGSYRRDASSSATTGRVRTDQL"
BASE COUNT         1155 a         1927 c         1883 g         1029 t
ORIGIN      
        1 atgcaggcgc ttgccgcggc cggcgtcgcg cctgccgtcc ccgtcgtcgc gctcgatgac
       61 agcgagggcg ggaccgcaca ttccgcctgg ctcgccccgc acggccagcg tcccgcgagc
      121 gtagcgatcg ctccggactg gccgttcaac atcatctatt cctccggcac caccggcacg
      181 cccaagggca tcgtgcagcc ccacgcgatg cgctgggcgc atgtgctgcg cggacgcatg
      241 aacggctacg acgacaccgc cgtcacgctg atctccacgc cgctgtactc gaacaccacg
      301 ctggtgagct ttctgccggc gatcgcgctc ggcggcagcg tcgtgctgat gccgaagttc
      361 gacgccgccg gctggctggc tctggccgaa aagcaccgcg ccacccatgc aatgctggtg
      421 ccggtgcagt accagcgcat catggcgcgg gaagatttcg aacgctacga cttgtcttcc
      481 ttccgcatga agttctgcac cagcgcgccc tttccggcag cgctcaaggc cgacgtcctg
      541 cgccgctggc cgggcgggct gatggagtac tacggcatga ccgaaggcgg cggcacctgc
      601 atcctcgccg cgcatgaaca tccggacaag ctgcacacgg tcggcaagcc ttcgcccggc
      661 cacgacatcc gcgtgatcga cgatgccggc aacgaggtcg cgcgtggcga agccggtgaa
      721 gtggtcggcc attctccggg catgatggtt ggctatcaca agcagccgga aaagacgcgc
      781 gaggcggaat ggtacgcgcc ggacggcaag cgcttcatcc gcaccggcga catcgggcgc
      841 ttcgacgagg acggcttcct ggtcctcatg gaccgcaaga aggacatgat catctccggc
      901 ggcttcaaca tctatccgag cgatctggag gccgtcctgc tgcgccatcc ctccattctc
      961 gaagccgcgg tgatcggcgt cccgtcggca cgctggggcg agaccccggt ggcggccgtc
     1021 gtcgcccgaa acggcacgtc actggaaccg gaacaggtgc tggaatgggc caatgcacag
     1081 cttggcaaga cccagcggct ggctgcggtc agcgtaacag accgtctgcc gcgcagcgcg
     1141 atcggcaagg tgctcaagcg cgagttgcgt gaagcgctgg cgcagcgcgg cgctttgcag
     1201 gtgccgtgac ctcggctagt ccgtcaacca gaacgcctgc agcagaccgg tgaccaccag
     1261 cgtgagaatc accgtcgccg cgatgttgag aaacagcccg gcccgcatca ttgtttgtat
     1321 cttcagctga ccggtcgcga acacgatggc gttcggcggc gtggcgaccg gcatcatgaa
     1381 cgcacagctc gccgcaatgg ccgccggcac ggtaagcagc agcggatcca cgccggtcga
     1441 catcgccagc gccccgagca acggcagaaa ggcggccgcg gtggcggtgt tggaggtcag
     1501 ctcggttagg aacacgatga ccagcacgac tgcgccggcc agcaggagca gcggcagcgc
     1561 actgaatacc gccagctgcc cggcaatcca tgaagcaagc cccgaattcc tgatgacatc
     1621 ggccagcgcc agcccgccgc cgaacagcag cagcacgccc catgggaggg ccttggcgtc
     1681 ctcccatacg agcaggcgcg cctgcgcctt gtccgcgctg ggaagcagaa acagcgtaat
     1741 gccggcggct atcgcgatga tggtgtccga cagccatccc acgccgtaac gggtgatgac
     1801 cggactcagt atccatgcag ctgcggtcac gacgaagact gccgccaccc gttgctccgc
     1861 tcggctgatc cggccttgcg cggccagttc gttcgtcagt gcctgcctgc cgccttcgcc
     1921 gctcttgatg cgaaatcccc ctcgcgtgag ccaccaccac gcgaccagca gcatgagcac
     1981 ggaaaccggc agcccgatga ccatccattg cgcaaacccg acctggatat cgtggttctt
     2041 cgccaggtaa gccgcgagca gcgcgttcgg cggcgtgccg atcagggttg cgatgccgcc
     2101 tatgctcgcc gcgtaggcta tggccagcag cagcgcagcc gcatagcggt ccacttcggc
     2161 acggtcctga tccgtgccga gcaggccgac gacggagatg ccgatcggca gcatcatcac
     2221 ggtggtggcc gtattcgata cccacatgct gagaaagccg gtcgccagca tgaatccggc
     2281 gatctgccgc tccggacgct ggccgaccag aagcagcacg ttcagcgcga tgcggcgatg
     2341 caggttccag cgctgcatcg cgatgccgat caggaagccg ccgagaaaca ggaaaatgat
     2401 cggattggcg tagctggctg tggtcgcgtc gagcttgcca aggccgagtg cgggcaccag
     2461 aacgatgggc agcagcgagg tggccgggat gggaatggcc tcggtcgccc accacgtcgc
     2521 catccacagc gccaggccgg tgcagcgcca cgcgtcttcc gacatgccgc ctggagcagg
     2581 aagaatcatc accgcaagca gccacagcgg ccccagccag aggccgatgc gcgcggcaga
     2641 cgaaggcttg gcttgcgcgt cagcagaggg tgtcattcgc aggtccttcg aaccggatgg
     2701 aggcaaggta caggcggttc ccggctgcgc ccgaagcgca ggccgcgtgc aaaccccgca
     2761 taagccattg accagttaca ccagacaaaa atccgggagg cggcggaccg gggcttgatc
     2821 cctgcaaaga cgcctgcctg gggcagcgtt gcgcaaaggc gccggtcctg ctgcgcttgc
     2881 gcccattcgc gccggggtgg cctgatgccg aagacgcctg cacgccctgc tcgcttgccg
     2941 catgaggggt gaacgcacaa gcacgcgaac ccaccggtga gaacgcaaca attgttccgg
     3001 ttcatcatat tccccgaatt gcggccatgg ttcccggcgg ttcctgtcag agcaccggca
     3061 ggcgatgcac gcggccgcgc gccattcggc gtcatcgaat ctgattcaaa cctgaaaaac
     3121 cacgtgtccg gaatctgcct cgcaggcatt ccgatttgtt cttcgtcgaa caagcggccg
     3181 actgtacttg ggctacccgt atgttgagga tatggttgcc tgacggcggt ctcccggccc
     3241 acatcacacc atacgccgaa gacgcatgct gacacacacc cgaaacaacc cgcccatcgc
     3301 gaccatcgcg gggaacttga tacgaagcgc tgtccacgcg gggagtacga atgaccgcgg
     3361 gcagcctgcc gtttccgcct tccaccagca tcacgcaatc cgaaagcaca tccgggagcc
     3421 catcatgaaa accgtgacct tcgaggctga acgcaagtcc acttcaatcc cgtcagcgtt
     3481 atcgacattc caccgcatcg gcaaagccga gctcaagacg atggaatcgc ggtggcgcgc
     3541 cgtcgaggca agaaccggag gaccgattga acaattcgac tgggccgtcg catgcgcgat
     3601 gcacaccgac gcttccgaag ttgaagtcgt cggcgtcatg cgcggcgagg agctccgtgc
     3661 cgtggtgccg ctcgcgatca agcgcatcgc aggtgtcaag cgcaggatga tgttgggcgt
     3721 cgacgagtat ttcgagccga tggattttct cttctcggat gcgcaatcgc tccagcgcct
     3781 gatgcagcaa ctggcaagag accggcgccc gatgctgctg tctcgcgttc cgaccgattc
     3841 cggcagcctc gacgcgctgc gccaggcatt caggaagcgc gcgctggtag tggaacggcc
     3901 gggtgaaggg tcgcccttca ttccgctcga tccgtcatgg tcggaacctg aacagcacct
     3961 caacgccggc cgccgctcgg atctgcggag gatgcggcgc cggctcgaca gcatgggcaa
     4021 ggccgaaagc gcgatccacg cgcccacacc ggatcaactc gacgccttgc tggaggaagc
     4081 cttccaggta gaagcccgtt cctggaaagg tgccgccggc accgctctgc tgtacgacgc
     4141 gaaaaggcgg aatcacctgc gcagctattt gaaggctgcc gcccgttcgg gcgaagtgcg
     4201 catcggattg ctaaggctgg acggccgcgc aattgcaatg gaaatcgcga tccagcgcgc
     4261 tgacgcgttg tggctgctga agatcggcta cgacgcggcg tttgcccact gcgcgccggg
     4321 acaacttctg ttgcgggaca ccattgaaca cgctgcccgg actggcttga agaactttga
     4381 attcctgggc gtgagcgcac cttggatcga tgtatggagc ttgcacaaac acgactgcgt
     4441 tgcggtgtgg atttatccgc cgaccgtcca aggcatgttg ggattcatgg ccgactcagg
     4501 ccttcggctc gcgcgcatgc ttcgacgcct ggcaataagt ggccgtcgtc gcctgatcaa
     4561 gagcatcgtc ccggtgctga acttcgctgc gaagtcgtac atcgcaggag aaacgcttgc
     4621 cgatgcgatg cgcgtggcgg aaaaagttcg cgcgaacgga gcgagcgtaa caatcgggta
     4681 ctggaatacc gaagacgaaa agcccgctga acttgcagag caggacgtgg ccgggcttga
     4741 ccaattggcg cattcgggaa gcgacgatta tctctcggtc aaactccccg cgctgcactt
     4801 caagaaggag ctcgtttcca agctcagtgg ggcggctgca aaggccggcc ggcgcattca
     4861 tttcgattcg cacggcattg aatacgtcgt caggaagatg gccgtctgcg aggagctgaa
     4921 gcggctccat ccggatctgg acatcgggtt caccttgcct ggccgatggg cgcgcagcgt
     4981 tgacgatgcg gactgggtgt gccggcaagg cctgtttgtc cgcgtggtca agggacagtg
     5041 gcccgatccc gcacaggtga acgccgatcc ggagcaggga ttcatgaacg tcatcgaacg
     5101 tttggcagga cgggcacgga aggttgcagt cgcgacgcac gatccgcgtt tggcggaaag
     5161 ggcgctgcga cgactgcaag cggcgggcac gccgtgtgaa atggaactgc tttacggatt
     5221 gccgttacgc cgctgcatgc aggtcgcacg cgagcgcggc gtgccgatgc ggatatacat
     5281 tccttacggt gaggcctatc tgccttatgc gctttctcag ttgcgagaca atccgcgtat
     5341 tctgtggtgg cttctgcggg acagcgtggc ggcattgcgg ccaggtgtcg gttcgtatcg
     5401 gcgtgacgcc tcttcatccg ccacaactgg acgcgtgcgt acagaccagc tgtagcgcca
     5461 gggacgccga agagggctac gcgcctgcgc cttccgcggc gcttcagtcg caatccttct
     5521 ttccccgccg ctcgctgtaa ccgttaggta cattcatgcg ttacttgggg aaacatttct
     5581 agctagaact attttcagac gagaaatctc ttattgcact cgcatgtgct ttttcgcatg
     5641 cggagttgtc aattaccaag gggattatct tgtttgatct gaatgctaga cgcttccgta
     5701 aatctgccat cgcctgtgcc tgctcggcgg tcctgcttgc cgcctgtgga ggcggtggcg
     5761 acgataccgg ttttgatcag atggcaatga acgacacgga gctcagcacc gacgtggatg
     5821 tggaggaagg ccgaaagaag ccatttccga ttaaccatcc gcatgcacaa gcgccggcac
     5881 ccgctccggc acccgctccg gcacctgctc cggcacctgc acctgcacct gcacctgcac
     5941 ctgcacctgc acctgcacct gcaccggcac ctgcaccggc accggcaccg gcac
//
LOCUS       DATLGF010000410         2014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_121019, whole genome shotgun sequence.
ACCESSION   DATLGF010000410 DATLGF010000000
VERSION     DATLGF010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121019"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VM406_12590"
     CDS             <1..417
                     /locus_tag="VM406_12590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL76846.1"
                     /translation="MQPSPEPGTKIVLDKYPMRVLVIEDNQDLAKLFCDLLEVMGCVT
                     YSAFSARSGVECARNEVPDLVFCDLRLPGEKSGFDVARELRGDPGLRHIPLVAVTGYD
                     DVREHQRARDAGFERVFEKPVKFAQILEVLNSYRKG"
     gene            513..1184
                     /gene="rpiA"
                     /locus_tag="VM406_12595"
     CDS             513..1184
                     /gene="rpiA"
                     /locus_tag="VM406_12595"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HVL76847.1"
                     /translation="MTQDEMKQAVARAAIEYVPSGEIIGVGTGSTANYFIDELGKIKD
                     RIKGAVASSEATAARLSAHGIAVLDLNEVESMSVYIDGADEITSQGAMIKGGGAALTR
                     EKIVASVARKFICIADGSKLVDTLGAFPLPVEVIPMAHAAVARALVGLGGQPKLREKD
                     GAPLVTDNGCYILDVTGLRIADPKGLEEQINNIVGVVTVGLFARRGADVALLGIADGV
                     RKLTF"
     gene            complement(1276..1548)
                     /locus_tag="VM406_12600"
     CDS             complement(1276..1548)
                     /locus_tag="VM406_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HVL76848.1"
                     /translation="MARMVQCIKLNREAEGLDFPPYPGELGKRIYENVSKEAWAAWLK
                     HQTMLVNENRLNLADTRARKYLAAQMEKHFFGEGADQAAGYVPPSE"
     gene            complement(1614..>2014)
                     /gene="argA"
                     /locus_tag="VM406_12605"
     CDS             complement(1614..>2014)
                     /gene="argA"
                     /locus_tag="VM406_12605"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HVL76849.1"
                     /translation="KLIEPLEADGTLVKRGRELIEREIEYFSVIEHDGVIFGCAALYP
                     FPAEKMAEMACLTVNPDVQGQGDGERILKHMENRARAAGFTKLFVLTTRTAHWFLKRG
                     FVLASVDDLPKDRRHMYNWQRKSLVLIKNL"
BASE COUNT          354 a          625 c          657 g          378 t
ORIGIN      
        1 atgcagccat ctcccgaacc cggcacgaag atcgtcctcg acaagtaccc gatgcgggtg
       61 ctggtgatcg aggacaatca ggaccttgcc aagctgtttt gcgacctgct ggaggtcatg
      121 gggtgcgtta cctactcggc gttcagcgcg cgcagcggcg tggaatgcgc ccgcaatgaa
      181 gtgccggacc tcgtcttctg cgacctgcga ctgccgggcg agaagagcgg ctttgacgtt
      241 gcccgcgagc ttcgcggcga cccggggctg cgccatatcc ccctggtggc cgtgaccggc
      301 tatgacgacg tacgtgaaca ccagcgtgcc cgcgacgccg ggttcgagcg cgtgttcgag
      361 aaaccggtca agtttgcgca gattctcgag gtgctcaaca gctatcgcaa gggctagcct
      421 gctgtttttt cggcggcagc ggcttccggc ggtacgggcc aggctggcgg gtttaaaata
      481 gccggctttg caagccttcc aggtccgcag ccatgaccca agacgaaatg aaacaggcgg
      541 tcgcccgcgc ggcgatcgaa tacgtgccca gcggcgaaat catcggagtc ggcaccggtt
      601 ccaccgccaa ttacttcatc gacgaactcg gcaagatcaa ggacaggatc aagggcgcgg
      661 tggcgtcttc cgaggccacg gcggcgcgcc tgtccgcgca cggcatcgcg gtgctcgatc
      721 tgaatgaggt ggagtccatg tccgtgtaca tcgacggcgc cgacgaaatc accagccagg
      781 gcgcgatgat caagggtggg ggcgccgcgc tgacgcgcga gaagattgtc gcttcggtgg
      841 cgcgcaagtt catttgcatc gccgacggct cgaagctggt cgacacgctg ggcgcattcc
      901 cgctgccggt ggaagtgatt ccgatggcgc acgcagcggt ggcgcgggcg ctcgtcgggc
      961 tcggcgggca gccgaagctg cgcgagaagg acggcgcgcc gctcgtgacc gacaacggct
     1021 gctacatcct cgatgtgacc ggcctgagga ttgccgatcc caagggactg gaagaacaga
     1081 tcaacaacat cgtcggcgtg gtcacggtag gcctgttcgc gcgccgcggc gcggatgtcg
     1141 cgctgctcgg tatcgcggac ggtgtgcgca agctgacatt ctgatcgcag cgcgacaaga
     1201 acgaaaagcc cggccgggca atccgggcgg gctttttttc atgctggcac acaacgaatg
     1261 cgctgccgtg tacgcttatt cggacggcgg aacgtaaccg gctgcctggt cggcgccttc
     1321 gccaaagaag tgcttctcca tctgggccgc aagatacttg cgcgcccggg tgtcggcgag
     1381 attcagccgg ttttcattga ccagcatcgt ctggtgcttg agccaggccg cccaggcttc
     1441 cttggacacg ttctcataga tgcgcttgcc gagttcgccg ggatacggcg gaaagtccag
     1501 gccttcggct tcgcggttca gtttgatgca ttggaccata cgggccattt cttacactcc
     1561 ttctcgaaag tttattggct tggctggcgg gccgacgcgc ggcccctcgg ccatcacaga
     1621 ttcttgatca gcaccagcga cttgcgctgc cagttgtaca tgtgccggcg atccttcggc
     1681 aggtcgtcga cgctcgccag cacgaagccg cgcttgagga accagtgcgc ggtgcgcgtc
     1741 gtgagcacga acagcttggt gaagcccgcc gcacgtgccc ggttctccat gtgcttcagg
     1801 atgcgctcac cgtcgccctg gccctggacg tcggggttga ccgtcaggca agccatctcc
     1861 gccatcttct cggccgggaa cgggtagagc gccgcgcagc cgaagatcac gccgtcgtgc
     1921 tcgatcaccg agaaatactc gatctcgcgt tcgatcagtt cgcggccgcg cttgaccagc
     1981 gtgccgtcgg cttccagcgg ctcgatcagc ttca
//
LOCUS       DATLGF010000411         3268 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_9514,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000411 DATLGF010000000
VERSION     DATLGF010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9514"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VM406_12610"
     CDS             <1..358
                     /locus_tag="VM406_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HVL76850.1"
                     /translation="VRTQAEILGAQPYDIPDTVLSHRGPACSFDAFLAEFDLQDPALA
                     ALADIVRAADTGTLETAPQAAGLLAISLGLGVIHRDDAALLDAAMPLYDALYAWCRHA
                     RNETHNWPFVGTGTQA"
     gene            355..1698
                     /gene="chrA"
                     /locus_tag="VM406_12615"
     CDS             355..1698
                     /gene="chrA"
                     /locus_tag="VM406_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HVL76851.1"
                     /translation="MKAAVAIPASVSLAEALLYWLKLGFISFGGPAGQIAIMHTDLVE
                     KRRWISEQRFLHALNYCMLLPGPEATQLAIYIGWLLHRTWGGIIAGVLFVLPSLLLLG
                     TLAWVYLVYGDAPLIAGILYGIKPSVVAIVLAAAWRIGARALKNRWLIALACAAFIAI
                     AILGVPFPAIVGAAALTGLIGGRLRPELFRLGQAHAGASTAHSGPAIIDDSTPTPAHA
                     RFTWGAMAATVGAGLLCGVGLLALLAWATDNTGTLTQMGWFFTKAALLTFGGAYAVLP
                     YVYQGAVETFQWVTPAQMIDGLALGESTPGPLIMIVAFVGFIGAWTQAVFGAEHPLLA
                     GMAGACVATFFTFLPSFIFILAGGPVVESTRDNVRLTAPLTAISAAVVGVIASLAVFF
                     AEHVFRLRLAPADWDWIAMAIAVIASYALLRQRIGTIPLIAASGAIGLVISWTLG"
     gene            1715..2356
                     /gene="mobA"
                     /locus_tag="VM406_12620"
     CDS             1715..2356
                     /gene="mobA"
                     /locus_tag="VM406_12620"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HVL76852.1"
                     /translation="MTATTAPRTCPEPGTAGKLPSVTGLVLAGGRGSRMGGVEKGLQA
                     FNGAPMLAEIVARLRPQVDELAINANRELDRYRAFGFPVRPDLRTGLPGPMAGIEAGL
                     AACTTDLLVVVPCDSPCLPPDLVCRLHAAMERRSAALAYAVTDSGQGEQAHPVFCMLD
                     RSLLPQLRAALDAGERRLGSWLRSQGAAPAVFSDEAAFRNINTFEELQGFQRT"
     gene            2440..>3268
                     /locus_tag="VM406_12625"
     CDS             2440..>3268
                     /locus_tag="VM406_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003268835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HVL76853.1"
                     /translation="MLDAVQPLTGSETAPLRQARMRVLAEALVSPIDVPAHDNSAMDG
                     YAFDGAKLTGGRMQLTVAGTALAGQPYKAVVKDGECVRIMTGAMMPPGCDTVIPLEAT
                     QSADGVVRFATADVKRGANRRLRGEDLARGQEALARGRLLRAADLGLAASLGVPALPV
                     LPRVRVAYFSTGDELREPGSRLDEGCVYDSNRYTLTALLEQTGCEVIDLGAVADDPKA
                     LADTLTRASMADAIITSGGAAAGDADHTAKVMASMGEVLFWRLAMKPGRPFAFGRIAG
                     "
BASE COUNT          469 a         1143 c         1098 g          558 t
ORIGIN      
        1 ggtacgcacc caggccgaga ttctcggcgc ccagccctac gacattcccg acaccgtgct
       61 ctcgcaccgc ggcccggcgt gcagcttcga tgcgttcctc gcggaattcg acctgcagga
      121 tccggcgctc gcggcgctgg ccgacatcgt gcgcgcggcc gacaccggaa cgctcgagac
      181 cgccccgcag gcggccggcc tgctcgcgat ctcgctcggg ctgggcgtga tccatcgcga
      241 cgacgccgcg ctgctcgacg ccgccatgcc gctctacgac gcgctctacg cctggtgcag
      301 gcacgcgcgc aacgagacgc acaactggcc gttcgttgga acagggactc aggcatgaag
      361 gccgccgtcg ctataccggc ctcggtttcg cttgccgagg ccctgctcta ctggctcaag
      421 ctcggcttca tcagcttcgg cggtccggcg ggccagattg cgatcatgca caccgatctt
      481 gtcgagaagc ggcgctggat ctccgagcag cgtttcctgc atgcactcaa ctactgcatg
      541 ctgctgccgg gaccggaagc gactcagctc gccatctata tcggctggct gcttcatcgc
      601 acctggggcg gcatcatcgc cggcgtgctg ttcgtgctgc cgtcgctgct cctgctcggc
      661 accctcgcct gggtctatct cgtctacggc gacgcgccgc tgatcgcggg gatactttac
      721 ggaatcaagc cgtcggtggt ggcgatcgtt ctggcggccg cgtggcgcat aggcgcgcgc
      781 gcgctgaaaa accggtggct gattgcgctc gcgtgcgcgg ccttcattgc aatcgcgatc
      841 ctcggcgtgc cgtttccggc tatcgtcggc gcggcggcat tgaccggctt gataggaggc
      901 aggctgcggc cggagctgtt tcgcctcggc caggcccatg caggcgcttc gaccgcgcac
      961 agcggcccgg ccatcatcga cgacagcacg cccacccccg cgcatgcgcg cttcacctgg
     1021 ggcgcgatgg cggcaaccgt gggcgccggg ctgctgtgcg gggtcggact gctggcgctt
     1081 ctggcatggg cgaccgacaa caccggcaca ctgacgcaaa tgggctggtt cttcaccaag
     1141 gcggcgctgc ttacgttcgg cggcgcctat gcggtgctgc cctatgtgta ccagggcgcc
     1201 gtggagacct ttcaatgggt cacacccgct cagatgatcg acggactggc attgggtgaa
     1261 agcacgcccg ggccgctgat catgatcgtc gccttcgtcg gcttcatcgg cgcctggacc
     1321 caggccgtgt tcggcgcgga acatccgctg ctggccggca tggccggggc ctgcgtcgcc
     1381 accttcttca cctttctgcc atcgttcatt ttcatcctgg ccggcggccc ggtcgttgaa
     1441 tcgacgcgcg acaatgtacg cctgaccgcg ccgctgaccg cgatctcggc ggcggtcgtc
     1501 ggtgtcatcg cgagcctcgc cgtgttcttt gccgaacatg tgttccgatt gaggctggcg
     1561 cccgccgact gggactggat tgcaatggcg atcgccgtca ttgccagcta tgcgctgctg
     1621 cgccagcgca tcgggaccat ccctctgatc gccgcgtccg gcgcgatcgg cctcgtgata
     1681 tcgtggacgc tcggatgagc cgaccgcatt gcccatgacc gccaccaccg caccccgcac
     1741 ctgtccggaa cctggcaccg ccggcaagct gccgtccgtc accggactgg tcctggctgg
     1801 cggccgcggt tcgcggatgg gcggggtcga gaagggtctg caagcattca acggcgctcc
     1861 gatgcttgcc gagatcgtcg cgcgactgcg cccgcaggtc gatgaactgg cgatcaacgc
     1921 caatcgcgaa ctggaccgct accgcgcatt cggttttccc gtgcgacccg atctgcgcac
     1981 cgggctgccg gggccgatgg ccggcatcga agcgggactg gccgcctgca cgaccgacct
     2041 gctggtcgtc gtcccttgcg attccccctg tctcccgccg gacctcgtct gccgcctgca
     2101 cgcggcaatg gagcggcgca gcgccgcact cgcctatgcg gtgacggaca gtgggcaagg
     2161 cgaacaggcg catccggttt tctgcatgct ggatcgctcg ctgctgccgc aactgcgcgc
     2221 ggcgctcgac gcgggcgagc gcaggctcgg ctcctggctg cgcagccagg gagcggcgcc
     2281 cgccgtgttc agcgacgagg cggcattccg caacatcaac acctttgaag aactgcaagg
     2341 atttcagcgc acatgagcct cccttccctg catgagcggg cggcacgcct gccgcattcc
     2401 ggcaccgaag acctcgccgt cgacctcgcc cgggatttca tgctcgacgc cgtgcagccg
     2461 ctcacgggca gcgagacggc accgctgcgg caggcgcgca tgcgggtgct ggctgaagcc
     2521 ctcgtttcgc cgatcgacgt tcccgcgcac gacaactcgg ccatggacgg ctacgcattc
     2581 gacggtgcaa agctgaccgg cggtcgcatg caactgaccg tggccggtac cgcgctggcc
     2641 ggacagccgt acaaggccgt tgtgaaggac ggcgagtgcg tgcggatcat gaccggggca
     2701 atgatgccgc ccggttgcga cacggtcatt ccgctggagg ccacccagag tgcggacggc
     2761 gtggtgcgtt tcgcgacggc ggacgtgaag cgcggcgcga atcgccgttt gcgcggcgag
     2821 gacctggcgc ggggccagga ggcgctggcg cgcggacgcc tgttgcgtgc cgccgacctc
     2881 gggctcgccg cctcgctcgg cgtgcccgcg ctcccggtgc tgccgcgcgt acgcgtcgcg
     2941 tacttttcca ccggcgacga attgcgcgag ccgggcagcc ggctcgacga aggctgcgtg
     3001 tacgacagca accgctacac cctcaccgcc ttgctcgaac agacgggctg cgaggtgatc
     3061 gacctgggcg cggtggccga cgacccgaag gcgctggccg acacgctcac ccgcgcgtcg
     3121 atggccgacg ccatcatcac ttccggcggc gccgcggccg gagacgccga ccatacggca
     3181 aaggtgatgg cgagcatggg cgaggtgctg ttctggcgct tggcgatgaa gcccggccgg
     3241 ccgttcgcat tcggacggat cgcgggcg
//
LOCUS       DATLGF010000412         2923 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_141534, whole genome shotgun sequence.
ACCESSION   DATLGF010000412 DATLGF010000000
VERSION     DATLGF010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141534"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VM406_12630"
     CDS             <1..617
                     /locus_tag="VM406_12630"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HVL76854.1"
                     /translation="PAPSPRLACPQCLRAARACICDLVTPVANEVELLILQHPDEVRH
                     AKGTARLLHLSLERSVLLTGEGFAEDMLQSRLAAPAGTTNLLLYPDTPGGRIAPPPAL
                     PPALPAPARLRLVLLDATWGKSLKMLLQNPVLQRLPRVAVNAAAPGRYAIRKARRPGQ
                     LSTLEAACHALAALEGAPQRYAGLLAAFDSFVAQRLAFIHDPAQ"
     gene            618..962
                     /locus_tag="VM406_12635"
     CDS             618..962
                     /locus_tag="VM406_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76855.1"
                     /translation="MHQNIAPARRSYLSISSAYGTQGGRMDIEQSKEQEAAGSGTSHS
                     GLLIGLAVGLGVLAWMASRQPRTRRYADRQEERRNPMHFFLAGSHPRRRAIDRSGHRP
                     LFERRQSVYDAY"
     gene            complement(983..2125)
                     /locus_tag="VM406_12640"
     CDS             complement(983..2125)
                     /locus_tag="VM406_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HVL76856.1"
                     /translation="MFGAFDRHNFGDLLFAHVAEALLAPRRLHFAGLIARDMRAVGGH
                     ALEALQVLGARLGAQPVNLLHAGGEVLTCTDEEAAVMLLEPSAAQALLSRGRTRVPLP
                     FAAARLPYIASPGQFPHVRMLFNAVGGVALDRLDPPLREEAAAALRTAAWLGVRDWRT
                     QAALRTAGIAARLLPDPATMVPALFAARIARHAAQGEVAAVRDVFPRGYLAVQFSSDF
                     GDDATLSLLASQLDEAAAAHRLGIALFRAGAAPWHDDLDVLARLHARMRCPVHVFESL
                     DIWDICALIGASVGFAGSSLHGRIVAAAFGLSRVSCTRLWPVPGKQDAYAHTWDTEWP
                     ACTGPAALAAAIAAALAVPAEARRQHAQAMQARYLAAFRDLAAALM"
     gene            complement(2152..>2923)
                     /locus_tag="VM406_12645"
     CDS             complement(2152..>2923)
                     /locus_tag="VM406_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76857.1"
                     /translation="TYSPLVAAFFAVHPDYVPPYAPKDRDACIWALSAGELNESQGFE
                     PLIFPLDAASYQPLIAPAFKNREEPGTVGVAMAIEHDPRIQLQQGAFTVHSSRTPLDR
                     LDGKERFLRKLLIPGAAIQAFCEELSLLGMRKSNLFPDLAALTSELRAAHPPAHPNRR
                     APAPQDSRAAVRVLELPEAVRRTRPTMEKLRMHEHGKDPEPDADGLENRNLTGATPPI
                     PDADDPARHGAPDEAAGLENSDVTRPNPPDSERATGNS"
BASE COUNT          448 a         1030 c         1018 g          427 t
ORIGIN      
        1 ctcccgcccc ttcccctcgc ctcgcctgcc cgcaatgcct gcgcgcggcg cgggcctgca
       61 tctgcgatct ggtgacgccg gtggccaacg aggtcgagtt gctgatcctg cagcacccgg
      121 atgaggtgcg ccatgcgaag ggcaccgcac gcctgctgca tctgtcgctg gaacgcagcg
      181 tgctgctgac gggcgagggg ttcgcggaag acatgctgca gagtcgtctg gcggcacccg
      241 ccggcacgac gaacctgctg ctctatccgg acacgcccgg cgggcgcatc gcgccgcccc
      301 cggcactgcc accggccctg cccgcgccgg ctcgattgcg cctcgtgctg ctcgatgcaa
      361 cctggggcaa gagcctgaaa atgctgctgc agaatccggt actgcagcgc ctgccgcgtg
      421 tcgctgtcaa tgccgcagca ccgggtcgct acgcgatccg caaggcgcgc cgcccgggac
      481 agctctccac gctggaagcc gcatgccacg cgctcgccgc gctggaaggc gcgccgcaac
      541 gctacgccgg cctgctggcg gcgttcgact ccttcgttgc gcagcgactt gcgttcattc
      601 atgaccctgc tcagtagttg caccagaaca ttgcgcccgc gcgccggtcg tacctgagca
      661 tcagcagcgc atacggcacg caagggggac gcatggacat cgagcaaagc aaggagcagg
      721 aggccgccgg gagcggcacg tcgcacagcg gtcttctgat cggccttgcg gtcggactgg
      781 gggtgctcgc ctggatggcg tcacgccagc cgcgcacacg ccgctatgcg gaccgtcagg
      841 aggaaagacg caacccgatg catttcttcc ttgcgggcag tcacccgcgc cggcgcgcga
      901 tcgaccgctc cggccaccgt ccgctgttcg aacggcgcca gtcggtctac gacgcgtact
      961 gatccgtatc tggcggcgtc tctcacatca gcgccgccgc gaggtcgcga aaggcggcca
     1021 ggtagcgcgc ctgcatcgcc tgcgcgtgct gccggcgcgc ttcggccggc accgccagtg
     1081 ccgcggcgat cgcggctgcc agcgccgccg gcccggtgca ggccggccat tccgtatccc
     1141 aggtatgcgc ataggcatcc tgcttgcccg gtaccggcca caggcgagta cagctcacgc
     1201 gggacaggcc gaaagctgcg gcgacgatgc gtccgtgcag gctgctgccg gcgaagccga
     1261 cgctggcgcc gatcagcgcg cagatatccc agatgtcgag cgactcgaat acgtgcaccg
     1321 ggcagcgcat gcgcgcgtgc agccgggcga ggacatcgag gtcgtcgtgc cagggcgccg
     1381 cgccggcgcg aaacagcgcg atgccgagcc gatgggcggc agccgcctcg tcgagctggc
     1441 tggccagcag cgacagcgtc gcatcgtcgc cgaaatcgct gctgaactgc acggccaggt
     1501 aaccgcgcgg gaacacgtcg cggacggccg ccacctcgcc ttgtgccgca tgccgcgcaa
     1561 tgcgcgctgc aaacagcgca ggcaccatcg tggccgggtc cggcagcagc cgcgccgcga
     1621 tgccggcggt gcgcaaggct gcctgggtgc gccagtcgcg cacgccgagc caggccgctg
     1681 tgcgcagcgc ggcagccgct tcctcgcgca gcggcggatc gagccggtcg agcgccacgc
     1741 cacccaccgc attgaacagc atccgcacgt gcggaaattg tccgggcgaa gcgatgtacg
     1801 gaaggcgcgc ggcggcgaac ggcaacggaa cgcgggtgcg gccgcgcgac agcaaggcct
     1861 gagcggcgga cggctcaagc agcatcacgg ccgcctcctc gtcggtgcag gtgagcacct
     1921 ctccgccggc gtgaagcagg ttcaccggct gcgcgccgag ccgggcgccg agcacctgca
     1981 aggcttccag cgcatggccg cctacggcgc gcatgtcgcg cgcgatcagc ccggcgaaat
     2041 gaagccggcg cggtgccagc agcgcttcgg cgacatgggc aaacagcagg tcgccgaagt
     2101 tgtgccggtc aaaggcgccg aacacgatga caggcgcgcc ccgcccggcc atcacgagtt
     2161 gccggtcgcc cgttcgctgt ccggcgggtt cggccgggta acgtcgctgt tttccagtcc
     2221 ggcggcttca tcaggcgcgc cgtgccgtgc cgggtcgtcg gcatccggta tcggcggcgt
     2281 ggcgccggtc aggttgcggt tttccaggcc gtcggcgtcc ggctcgggat cttttccatg
     2341 ctcatgcatc cgtaatttct ccatcgttgg ccgggtacgc cgtaccgctt ccggcagctc
     2401 aagcacgcga accgcggcac ggctgtcctg cggcgcgggc gcgcgcctgt tcgggtgggc
     2461 tggcggatgc gcggcgcgca gttccgaggt cagggccgcg agatccggga acagattgga
     2521 cttgcgcatg ccgagcaggc tcagttcctc gcagaatgcc tgtatcgctg cccccggtat
     2581 cagcagcttg cgcaggaagc gttccttgcc gtccagccgg tcgagcggcg tgcgcgacga
     2641 atgcacggtg aacgcgccct gctgcagctg gatgcgcgga tcgtgctcga tcgccatcgc
     2701 cacgccgacc gtgcccggct cttcccggtt cttgaacgcg ggcgcgatca gcggctggta
     2761 ggacgccgca tccagcggaa agatcagcgg ttcgaatccc tgcgactcgt tgagctcgcc
     2821 ggccgacagc gcccagatgc aggcgtcgcg atccttcggc gcgtagggcg gcacgtaatc
     2881 gggatgcacg gcgaagaagg ccgcgaccag cggcgaatag gtc
//
LOCUS       DATLGF010000413         2868 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_183681, whole genome shotgun sequence.
ACCESSION   DATLGF010000413 DATLGF010000000
VERSION     DATLGF010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183681"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..133
                     /locus_tag="VM406_12650"
     tRNA            43..133
                     /locus_tag="VM406_12650"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:77..79,aa:Ser,seq:gga)
     gene            complement(338..1162)
                     /locus_tag="VM406_12655"
     CDS             complement(338..1162)
                     /locus_tag="VM406_12655"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL76858.1"
                     /translation="MTTFRRSILATVACAVALSSPAWAQTAAPAAPAYEPKVGQAGKD
                     VIWVPTPQTVVDRMLEMAELKADDFLVDLGSGDGRTVITAAKRGVRAHGIEYNPDMVV
                     LSKRAAEAEGVAGRATFEQADIFKSDFSHATVVTLFLLPQLNLRLRPTLLDMKPGTRV
                     VSNSFTMDDWEPDESDEVKVGCPNYCSVYKWTVPAKVEGTWQLGNKQLVLTQTFQKLE
                     GALREGGKATPISDARMDGARIIFTAGKERYAGEVDGRSMKGTINGRTAWSATRSN"
     gene            1379..1666
                     /locus_tag="VM406_12660"
     CDS             1379..1666
                     /locus_tag="VM406_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76859.1"
                     /translation="MAGACTKHGERADKLVASMRPAVDDSDGLMRDALPRDAVVIAGG
                     NRAGAGEPSCAARCAGHAGSRIIGCSPGPAWVPDLQPLAAWNEAEEEWRSR"
     gene            1684..2853
                     /locus_tag="VM406_12665"
     CDS             1684..2853
                     /locus_tag="VM406_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVL76860.1"
                     /translation="MDFSVPEEFRDLQARIRSFVAERVIPYERDARLGPHGPDESLRR
                     ELIAHARAAGLLSPHVNEEYGGLGLSHRGRAIAFEEAGYSLLGPVALNVFAPDEGNMH
                     LLEAVATPEQKERWLRPLAAGEIRSCFCMTEPPPGAGSDPSMLKTTAVLDGDDYVING
                     LKWFTTGADGATLAIIMAMVPDLGATMFLSDMAAPGIVIERSLDTLDTCFPGGHGVVR
                     FENLRVPASAILGEAGQGFRYAQLRLSPARLTHCMRWLGAARRAHDVAVDYARGREAF
                     GKPLGAHEGVGFMLADNEMDMHLARLTIWHCAWVLDEGQLALTESSRAKVIVSEAVWR
                     VADRCVQILGGQGVSGETVVARIFADMRAFRIYDGPSEVHRWSLAKRIMRGQTQG"
BASE COUNT          452 a          924 c          983 g          509 t
ORIGIN      
        1 acctgctata atctgcggct tcgcgcaagc gacccgaatc caggagaggt ggatgagtgg
       61 tttaagtcgc acgcctggaa agcgtgtata ggttcatagc ctatcggggg ttcgaatccc
      121 cccctctccg ccaagaatca agcaaacaag cgcctttcgg ggcgcttttt tgttgctcct
      181 cgctctctcc acggcgcttc gcggcggtgt cgcatcgcct cctctatgca tcgcttagcc
      241 ggtgactcgt gcgtgcttag ccaacgcgcg ccgggaaatg acacagggcc cgctgtgcgg
      301 gccctgtgtc aggatatacc gccgaactgt gaagccgcta attcgaacgc gtcgcgctcc
      361 acgcggtgcg gccgttgatc gttcctttca tcgagcggcc gtccacctcg cccgcatagc
      421 gctccttgcc cgcggtgaag atgatgcgcg ccccgtccat gcgggcatcg ctgatcggcg
      481 tagctttgcc accctcgcgc agcgcgccct cgagtttctg gaaggtctgg gtgagcacca
      541 gctgcttgtt gccgagctgc caggtgcctt ccaccttggc cggtaccgtc cacttgtaga
      601 cgctgcagta gttcgggcag ccgaccttca cctcgtcgga ctcgtccggt tcccagtcgt
      661 ccatcgtgaa cgagttggac acgacgcgcg tgcccggctt catgtccagc agcgtgggcc
      721 gcagccgcag attgagctgc ggcagcagga atagcgtgac gaccgttgcg tgcgaaaaat
      781 cggacttgaa gatgtcggcc tgttcgaagg tcgcgcggcc ggccacgccc tcggcttcgg
      841 cggcgcgctt ggacagcacg accatgtccg ggttgtactc gatcccatgc gcacgcacgc
      901 cgcgcttggc ggcggtgatg accgtgcgtc cgtcgccgga gcccaggtcg acgaggaagt
      961 cgtcggcctt cagctcggcc atctcgagca tgcggtccac cacggtctgc ggggtcggca
     1021 cccagatcac atccttgcca gcctggccga ccttgggttc atacgcgggt gccgccggcg
     1081 ctgctgtctg cgcccaggcg gggctgctca gtgcaacggc acatgccacg gtggcgagga
     1141 tggatctgcg gaatgtcgtc atcaagaatc tcctggttaa cggatcagaa acgggacaga
     1201 aggtaacagc tgggacggtg ggatctgcag gagtctaccg tttccggccc agcgttgctt
     1261 atcgtgctgt caacgcaccg cacgcgcggc gaagtggaga tcttcgcgac gccgtcggcc
     1321 gcgcgggatg cgaccctcgc tgccgccgtc gagcgcagtc cggccaccga ttccgaccgt
     1381 ggcgggcgcg tgcacgaagc acggcgaacg cgcggacaag ctggtcgcgt cgatgcgtcc
     1441 ggccgtcgac gacagcgacg gcctgatgcg cgacgcgttg ccgagagatg cggtggtgat
     1501 cgccggcggc aatcgagcgg gcgctggcga accgtcctgc gctgctcgat gtgctggtca
     1561 cgccggaagc cgtatcatcg gatgttcgcc cgggcctgcc tgggtacccg acctgcagcc
     1621 gctggcggca tggaatgaag ccgaagaaga atggagatca cgatgaacca gcagcatggc
     1681 ggcatggatt tttccgtgcc cgaggaattc agggacttgc aggcgcgcat ccgcagcttc
     1741 gtcgccgaac gggtgatccc gtacgagcgc gatgcacgcc tcggcccgca cgggccggac
     1801 gagtcgctgc ggcgcgagct gattgcgcac gcgcgcgcgg ccggtctgct gtcgccgcat
     1861 gtgaacgaag agtacggcgg cctcggcctg tcgcatcgcg gacgggcgat cgcattcgag
     1921 gaggccggct attcgctgct gggcccggtc gcgctgaatg tatttgcgcc ggacgagggc
     1981 aacatgcatc tgctggaagc tgtcgccacg ccggagcaga aggagcgctg gctgcgtccg
     2041 ctcgccgcgg gcgagatccg ctcctgcttc tgcatgaccg agccgccgcc cggcgccgga
     2101 tccgatccgt cgatgctgaa gaccaccgcc gttcttgacg gcgacgacta cgtgatcaac
     2161 ggcctgaaat ggttcaccac cggcgccgac ggcgcgacgc tggcgatcat catggcgatg
     2221 gtgccggacc tgggcgcgac gatgttcctg tccgacatgg cggcgccggg catcgtgatc
     2281 gaacgctcgc tggacacgct cgatacctgc tttcccggtg gccatggcgt ggtgcgtttc
     2341 gagaacctgc gcgtgccggc ctcggccatc ctcggcgaag cggggcaggg cttccgctac
     2401 gcgcagctgc gcctgtcgcc ggcgcggctg acgcactgca tgcgctggct cggcgcggcg
     2461 cgccgcgcgc atgatgtcgc ggtcgactac gcgcgcgggc gcgaggcctt cggcaagccg
     2521 ctcggtgcgc acgaaggcgt tggcttcatg ctggccgaca atgagatgga catgcacctc
     2581 gcgcgcctga cgatctggca ctgcgcatgg gtgctggacg agggacagct cgcgttgacc
     2641 gaatcgagcc gggcgaaggt gatcgtttcc gaggcggtct ggcgcgttgc cgaccgctgc
     2701 gtgcagatcc tcggcgggca gggcgtgtcc ggtgagaccg tggtggcacg catcttcgcc
     2761 gacatgcgcg cattccgcat ctacgacggc ccgtcggagg tgcatcgctg gagcctggcc
     2821 aagcggatca tgagagggca gacgcagggc tgagcccggg tgcaaatg
//
LOCUS       DATLGF010000414         6021 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_232631, whole genome shotgun sequence.
ACCESSION   DATLGF010000414 DATLGF010000000
VERSION     DATLGF010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232631"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VM406_12670"
     CDS             complement(<1..336)
                     /locus_tag="VM406_12670"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HVL76861.1"
                     /translation="MKKSLFALAVLGAFAGAASAQTSVTVYGVADISLAKARGVSTQM
                     SSAGLLNNGTSRLGFRGTEDLGGGLRASFNFEQGIDLEQGNLSQDGGGAWGRAANMSL
                     GGAWGTLSMG"
     gene            576..1202
                     /gene="coq7"
                     /locus_tag="VM406_12675"
     CDS             576..1202
                     /gene="coq7"
                     /locus_tag="VM406_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041321988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HVL76862.1"
                     /translation="MSIFDSFIVQADKMLRTVSGVAHASRPSPAGRVAEGELAGADRR
                     HSAGLMRVNHVGEVCAQALYEAQGRFSRSPELRAQFAQAGREEEDHLAWTSERIRELG
                     SHTSLLNPFWYAGAYVLGAVAAKVGDAASLGFVVETERQVEAHLASHMERLPAQDMKS
                     RAIVEQMRVDEIEHGSKAQALGAAQTPAPVRFGMRAMAKVMTTTAYYV"
     gene            complement(1227..1682)
                     /locus_tag="VM406_12680"
     CDS             complement(1227..1682)
                     /locus_tag="VM406_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HVL76863.1"
                     /translation="MECTVRWAGSPGMTFLAETGSGHLVTMDGAPEGGGRDLAPRPME
                     LVLAGTGGCTAYDVVLILRKSQQDVRGCDVRLSAERAEKDPKVFTKVHFHFTVRGRSL
                     KPNVVERAIKLSHEKYCSASIMLEKTAAITHSFEIVDDLAAAEAAPSTV"
     gene            2111..2539
                     /gene="rplM"
                     /locus_tag="VM406_12685"
     CDS             2111..2539
                     /gene="rplM"
                     /locus_tag="VM406_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HVL76864.1"
                     /translation="MKTFSAKPHEVQREWFVIDATDKVLGRVASEVARRLRGKHKPEF
                     TPHVDTGDYIVVINAGKLRVTGAKEIDKKYFRHSTYPGGIYETNFRKMQERFPGRALE
                     KAVKGMLPKGPLGYAMIKKLKVYAEDSHPHAAQQPKALEI"
     gene            2549..2941
                     /gene="rpsI"
                     /locus_tag="VM406_12690"
     CDS             2549..2941
                     /gene="rpsI"
                     /locus_tag="VM406_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HVL76865.1"
                     /translation="MIGNYNYGTGRRKSAVARVFIKAGSGNIVVNGKPAAEYFSRETG
                     LMVIRQPLELTGNTERFDIMVNVHGGGESGQAGAVRHGITRALIDYDAGLKPELAKAG
                     FVTRDAREVERKKVGLRKARRAKQFSKR"
     gene            3077..4120
                     /gene="argC"
                     /locus_tag="VM406_12695"
     CDS             3077..4120
                     /gene="argC"
                     /locus_tag="VM406_12695"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HVL76866.1"
                     /translation="MIKVGIVGGTGYTGVELLRLLAAHPEVELTAITSRKEDGLPVAD
                     MYPSLRGHVSLAFSTPEKADLAGCDVVFFATPHGVAMSQARELLAAGVKIIDLAADFR
                     LKDVAEFEKWYGMPHACPEILAEAVYGLPEINREAIRRARVIGLPGCYPTSMQLGYAP
                     LLAHGKPLVNESALIADCKSGVSGAGRKAEVHTLLAEAADNFKAYAVKGHRHLPETTQ
                     GLQAIAGRKVGLTFVPHLVPMIRGIHSTLYATILPEARDVDYQALYENHFRDEYFVDV
                     MPAGSHPETRSVRASNKVRIAVHRPEGGDLLVILVVEDNLVKGAAGQGVQCMNILFGL
                     AESTGLMHIPVLP"
     gene            4167..4895
                     /locus_tag="VM406_12700"
     CDS             4167..4895
                     /locus_tag="VM406_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HVL76867.1"
                     /translation="MKSKFWRRRRMVSSPRVTIRTPWSWSTRVGFMLAVVATSAAAGI
                     WLFENSRAFAGVSVEDARQQLISYRDQVTRLSAERDQLSASANSAESELNIERAAQRQ
                     LLAQVKALEAENARLKEDLAFFDSLLPNASAPQGIAIRRLKIDQVAPNQLSYRVLVMQ
                     GGRGDHFSGVLQLVITTVQDGKTAMMVFPEETSAEPGRFKLGFRHYQRLEGVLALPEG
                     TTATMVSARVLDKGQIRAQTSVNL"
     gene            4908..5297
                     /locus_tag="VM406_12705"
     CDS             4908..5297
                     /locus_tag="VM406_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HVL76868.1"
                     /translation="MLGRKSKSTIDSLIGICTNIEGNVHFKGGLRIDGHVKGNVIADT
                     EEPSMLVISEHAKVEGEVRVAHLVINGEVVGPVHSSELLELQPRARITGDVYYKALEM
                     HGGALVAGKLTHDQVGEPVLKLALSNT"
     gene            5355..5705
                     /gene="erpA"
                     /locus_tag="VM406_12710"
     CDS             5355..5705
                     /gene="erpA"
                     /locus_tag="VM406_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HVL76869.1"
                     /translation="MNAVTEMPDPIMFSDSAAAKVAQLIEEEGNPDLKLRVFVQGGGC
                     SGFQYGFTFDEIVNEDDTTMTKNGVQLLIDSMSYQYLVGAEIDYKDDIEGAQFVIKNP
                     NATTTCGCGSSFSA"
     gene            complement(5775..>6021)
                     /locus_tag="VM406_12715"
     CDS             complement(5775..>6021)
                     /locus_tag="VM406_12715"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HVL76870.1"
                     /translation="VYVCGGGACNATLLAALTRNLQQHGAHIRVDTTAILGVEPQLVE
                     ALAFAWLAHRHVARLPGNLPSVTGARGPRILGALYPA"
BASE COUNT         1182 a         1739 c         1953 g         1147 t
ORIGIN      
        1 gcccatcgac agggtacccc atgcgccgcc cagcgacatg tttgcagcac ggccccaagc
       61 gccgccaccg tcttggctca ggttaccttg ctcgaggtcg atgccttgct cgaagttgaa
      121 cgaagcgcgc aggccgccac cgaggtcttc ggtgccgcgg aagcccagac ggctggtgcc
      181 gttgttcagc aggccagcgg aggacatttg cgtcgagacg ccacgagcct tggccaggga
      241 gatgtcagca acgccgtaga cggtaaccga agtttgtgcg gaagctgcac cagcaaatgc
      301 gcccagaact gcgagagcga aaagcgactt tttcatcaaa aaatctccaa aagttgagac
      361 gctagtatat ttcttggttt tttgcggcaa ggccactagt ggttacgtcc ctgccgcact
      421 tcccggtcgt cgaacttgtt gattcaggaa gctgtggggt atttcaacaa agatcagggg
      481 tgtgcgcaaa agatatttgg tcgtattgtt aattgagcga cgcttccgtt gtatcagcgc
      541 aacggagtgt tgcctttgtg aacaggaatt cactgatgag cattttcgat agtttcattg
      601 ttcaggccga caagatgctg cgcacggtct ccggcgtcgc gcatgccagc cgtccaagcc
      661 cggcagggcg tgtcgcggag ggggagttgg ccggcgccga caggcggcac agtgccggcc
      721 tgatgcgggt caaccacgtg ggcgaggtgt gcgcccaagc cctgtacgag gcgcagggac
      781 ggttttcccg gtcgcccgag ctgagggcgc agtttgcgca ggcgggcagg gaggaggagg
      841 atcatctcgc ctggacatcc gagcgcatcc gcgaactggg ctcgcacacc agcctgctca
      901 atccgttctg gtatgcggga gcctacgtgc tcggggcggt cgccgccaag gtgggggacg
      961 cggcgagcct cggcttcgtc gtcgagacgg agcggcaggt ggaggcgcac ctcgccagcc
     1021 atatggagag attgcccgcg caggatatga agtcgcgcgc gatcgtcgag cagatgcgcg
     1081 tcgatgaaat cgagcacggg tcgaaggcgc aggcgctggg ggccgcgcag acccccgcgc
     1141 cggttcgctt cgggatgcgt gcgatggcca aggtgatgac gacgactgct tactatgtct
     1201 gatccagagc ccccggcaag agagcactaa acggtcgacg gggcggcctc cgcagccgcg
     1261 aggtcgtcga cgatttcaaa cgagtgcgtg atggctgcgg tcttctcgag catgatcgag
     1321 gcggagcaat atttttcgtg ggagagcttg attgcgcgtt ccacgacatt gggcttcagg
     1381 ctgcgcccgc gcaccgtgaa gtggaagtgc accttggtga acactttcgg gtctttctcc
     1441 gcacgttccg ctgagaggcg cacgtcgcag ccgcgcacgt cctgctggct cttgcgcagg
     1501 atgaggacga cgtcataggc agtgcatccg cccgtgcccg ccagcaccag ttccatcggg
     1561 cgcggcgcca gatcgcgccc gccgccttcc ggcgcgccgt ccatcgtcac gaggtggccc
     1621 gaaccggtct cggccaaaaa ggtcatgccg ggagagccgg cccagcgcac tgtgcattcc
     1681 atgtgaagcc cttcgttctt gtaaagcggc tattgtagcc ctgcgggatt cccgccgcgc
     1741 ggcggcagcg gtaatgacgc agctgcagca tggggagtgt gcaaggcgga atggcgattc
     1801 ctgttgcagg gtgaagacgg ttttcttgca ttgcacaaaa atttaggcta ggatggaggc
     1861 gttggtcgag aatgcttctc gttttcgacc tgccaatgtc tcctccaccc tcctcctttg
     1921 gtgtggattg aacccgaagc ctcgcagctt cgggtttttt ttgcctggaa tcgatgtttg
     1981 acgccaagtc tttgaaaagg ctatactttc aggctttccg ttcgctcagg aaaagataac
     2041 aaggtcgagt ccgtgggaat ggcctgcgga accgccagca tttgagcgtg aaatcatcaa
     2101 ggacaacatc atgaaaacct tttccgcgaa gccccatgag gttcagcgcg agtggttcgt
     2161 gattgacgca acggacaagg tcctcggtcg tgtcgccagc gaagtggcac gccgactgcg
     2221 cggcaagcac aaacccgagt tcactcccca cgtcgacacg ggcgactaca tcgtcgtcat
     2281 caacgcaggc aagctgcgcg tgaccggtgc gaaggaaatc gacaagaagt acttccgcca
     2341 ctcgacctat ccgggtggta tctatgaaac gaacttccgc aaaatgcagg agcgtttccc
     2401 cgggcgcgcg ctggagaagg ctgtcaaggg catgctgccc aagggcccgc tcggctacgc
     2461 aatgatcaag aagctgaagg tgtacgcgga agactcgcat ccgcacgccg cccagcagcc
     2521 caaggcactc gaaatctaag gagcggacat gatcggtaac tacaactacg ggaccggccg
     2581 ccgcaagagt gctgtggcgc gcgtcttcat caaggcaggc agcggtaaca tcgtcgtcaa
     2641 cggcaagccg gcagccgaat acttctcccg cgaaaccggc ctgatggtga tccgtcagcc
     2701 gctggaactg accggcaaca ccgagcgttt cgacatcatg gtcaacgtgc atggcggcgg
     2761 cgaatccggc caggcgggtg cggtgcgtca tggcatcacc cgtgcgctga tcgactacga
     2821 tgcgggcctg aagccggaac tggcgaaggc tggtttcgtc acgcgcgatg cgcgtgaagt
     2881 ggagcgcaag aaggtcggcc tgcgcaaggc acgtcgcgcg aagcagttct cgaagcgtta
     2941 atcggcgctt cgccggggct gcggtcccgg caaacgaaaa gccgcctggg gcaacccggc
     3001 ggcttttttg ttgccgaagg tttttcgcgg ctgctttaga atttcagttt tcctatttca
     3061 ctctgaagga acgggcatga tcaaggtggg tatcgtaggc gggaccggct ataccggcgt
     3121 ggagctgctg cgcttgcttg cagcgcatcc ggaagtcgag ctgacggcca tcacctcccg
     3181 caaggaggac gggctgccgg tggcggacat gtatccgtcc ttgcggggtc atgtctccct
     3241 ggccttttcc acgccggaga aggccgatct tgccggctgc gacgtggtgt tcttcgccac
     3301 gccgcacggc gttgcgatgt cgcaggcgcg cgaactgctc gcggccggcg tgaagatcat
     3361 cgatctggcg gcggacttcc ggctcaagga tgtcgccgag ttcgagaagt ggtacggtat
     3421 gccgcacgcc tgcccggaga ttctcgcgga agccgtgtac ggcctgcccg agatcaaccg
     3481 cgaggcgatc aggagggcgc gtgtgatcgg gttgcctggg tgttatccga cttcgatgca
     3541 gctcggctac gcgccgttgc tggcgcacgg caagccgctg gtcaatgaat cggcgctgat
     3601 tgccgactgc aaatccggcg tgtcgggcgc cgggcgcaag gcggaagtcc ataccttgct
     3661 cgcggaagcg gccgacaatt tcaaggccta tgccgtcaag ggacaccgcc atcttccgga
     3721 aaccacgcag ggcctgcagg cgatcgccgg ccgcaaggtc ggcctgacct tcgtgcctca
     3781 cctggtgccg atgatccgcg gtattcattc cacgttgtac gcgaccatct tgccggaggc
     3841 gcgcgacgtc gactatcagg cgttgtacga aaaccacttc cgcgacgagt acttcgtgga
     3901 tgtgatgccg gccggcagcc atcccgagac gcgctcggtg cgcgcttcca acaaggtgcg
     3961 catcgccgtc catcgtcccg aaggcggcga tctgctggtc atcctcgtgg tggaggacaa
     4021 cctcgtgaag ggtgcggccg gtcagggtgt gcaatgcatg aatatcctgt ttggccttgc
     4081 cgaatcgacg ggattgatgc acattccggt attgccctag atcaagagcg ctgtcgaaac
     4141 agcgctcgtc atcaccacgg agtcgcatga agtccaagtt ttggcgccgc cggagaatgg
     4201 tgtcgtcgcc gcgcgtgaca atccgcacgc cctggtcctg gtccacccgc gtcggcttca
     4261 tgctcgcggt cgttgcaacg agtgccgccg caggaatctg gctgttcgag aacagccggg
     4321 cctttgccgg ggtgagtgtg gaagacgcgc gtcagcaact catcagctat cgcgatcagg
     4381 tcacgcgctt aagtgcggag cgcgatcagc tctcggcgag cgcgaattcc gccgagagcg
     4441 aactcaacat cgagcgggcc gcacagcggc agttgctcgc ccaggtcaag gcgctggaag
     4501 ccgagaatgc ccggctgaag gaagaccttg cattcttcga cagcctgctc ccgaacgctt
     4561 ccgccccgca aggaattgcc atcaggcgat tgaagatcga tcaagtcgcg ccgaaccagt
     4621 tgagctatcg tgtgctggtc atgcaggggg gcagggggga ccacttctcc ggagtgttac
     4681 agcttgttat cacgacggtg caggacggta aaactgctat gatggttttc cctgaggaaa
     4741 catcggcaga gccgggccgg ttcaagctgg gattccgtca ctaccaacgc ctggagggcg
     4801 tgcttgcgct gcccgagggc accactgcca cgatggtgtc ggcccgcgtg ctcgacaagg
     4861 gccaaatccg tgcgcaaact tccgtgaatt tgtagaggag atccatcatg ctaggccgca
     4921 agagcaaaag cacgatcgac agcctgatcg gcatttgtac gaacatcgag ggcaacgtcc
     4981 acttcaaggg ggggctgcgc atcgacggtc atgtgaaggg caatgtcatt gcggacacgg
     5041 aagagccgag catgctggtc atttccgaac atgcgaaggt agagggcgag gtgcgcgtgg
     5101 cgcatctggt catcaatggc gaagtcgtcg ggccggtaca ttcctccgag ctccttgaat
     5161 tgcagccgcg cgcccgcata accggagatg tgtactacaa agcgctcgaa atgcatggcg
     5221 gggcgctggt ggcgggcaag ctcacgcatg accaggtcgg ggaaccggtt ctgaagctgg
     5281 cgctgtccaa tacgtgaaaa gcgccgtgcg actataatcg aggcattgag ttttgcttcg
     5341 caggagcccc caacatgaat gctgttaccg aaatgcccga tccgatcatg ttctcggaca
     5401 gtgccgcggc gaaagttgcc caactgatcg aggaagaggg gaacccggac ctgaagctgc
     5461 gcgtgttcgt gcagggtggc ggttgctccg gtttccagta cggtttcacc ttcgacgaga
     5521 tcgtcaatga ggacgatacg acgatgacca agaacggcgt ccagctgctg atcgactcga
     5581 tgagctacca gtatctggtc ggcgccgaga tcgattacaa ggatgacatc gaaggtgcgc
     5641 agttcgtgat caagaacccg aacgccacca ccacttgcgg ctgcggctcg tcattctctg
     5701 cctgagccgt cgtgatccga aaaaagcgca acctcggttg cgcttttttt tcgcccgctg
     5761 ctcaggtgag atgctcaggc gggatagagg gcgccgagaa tgcgcgggcc gcgcgcgccg
     5821 gtgacgctcg gcagattgcc aggcaggcgc gccacatgac ggtgggcgag ccaggcgaag
     5881 gcaagtgctt cgaccagttg cggctccacg cccagtatcg ccgtggtgtc gacccggatg
     5941 tgggcgccgt gttgctggag attgcgggtg agcgcggcga gcagggtagc gttgcaggcg
     6001 ccgccgccgc atacatagac c
//
LOCUS       DATLGF010000415         2560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_121350, whole genome shotgun sequence.
ACCESSION   DATLGF010000415 DATLGF010000000
VERSION     DATLGF010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121350"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1637
                     /locus_tag="VM406_12720"
     CDS             <1..1637
                     /locus_tag="VM406_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVL76871.1"
                     /translation="VVFTPYIGYVLLKVKPHGEGGGHHDVFDGPFYQRFRALVNWCVE
                     WRKTTIVLTLFVFGLGVFGFRFIEQQFFPDSSRPELMVELWLPEGSSFAATEAQAKKF
                     EAFLASQDGVLSTTSYVGTGSPRFYLPLDQIFPQTNVAQIVVLPRDLKAREALRLKIV
                     DVFKNDFPEVRGRVKMLPNGPPVPYPVQFRVTGPEVEVVRVIADEVKEVMRANPNAVG
                     VNDNWNESVKVLRLDLDQDRLRALGVSSQSVMRTANTILSGSPIGQFRDHDKLIDIVV
                     RQPVEERSTLNALDNAYIPTTNGRSVPMSQVARAGFVWEPGVVWREGREWAITVQSDV
                     VDGIQGPTVSSQIDPQLNKLRAVLPPGYRIELAGAAADSGKAQESIAANIPLVIFIIF
                     TLLMLQLQSFSRSVLVFLTGPLGIAGAAMALLLLQRPFGFVAQLGVIALFGMIVRNSV
                     ILVDQIEQDIRNGAAPWDAIVEAAVRRCRPIVLTAAAAVLAMIPLSRSVFWGPMAVAI
                     MGGLIVATALTLLFLPALYATWFRVKKPDPALQTIP"
     gene            1827..1913
                     /locus_tag="VM406_12725"
     tRNA            1827..1913
                     /locus_tag="VM406_12725"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1861..1863,aa:Leu,seq:tag)
     gene            1949..>2560
                     /gene="tig"
                     /locus_tag="VM406_12730"
     CDS             1949..>2560
                     /gene="tig"
                     /locus_tag="VM406_12730"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HVL76872.1"
                     /translation="MATAVETLDKLERRMTITVPLADVQAEVEKRLKIRAKTAKAPGF
                     RPGKVPMKMVAQQYGYQVETEVLNDKVGRAFSEAASENNLRVAGFPKIEPKTGDGVAE
                     GMVAFDATFEVYPEVKVGDLSSADVEKVTSAVTDAEIDKTIDILRKQRVHYHTKGEHG
                     AHGDGGADTSAQTGDRVTLDFVGKIDGVEFQGGKAEGFSFVLGE"
BASE COUNT          460 a          811 c          841 g          448 t
ORIGIN      
        1 cggtcgtatt caccccgtac atcggttatg ttctgctgaa ggtcaagccg cacggggagg
       61 gcggcggcca ccacgacgtg ttcgacggac cgttctacca gcgcttccgc gcgctggtga
      121 actggtgcgt ggaatggcgc aagaccacga tcgtgctcac gctcttcgtg ttcgggctcg
      181 gcgtgttcgg cttccgcttc atcgagcagc agttcttccc cgattccagc cgccccgagc
      241 tgatggtcga actgtggctg cccgaaggca gctcgttcgc ggccaccgag gcgcaggcga
      301 agaaattcga agcctttctc gccagccagg acggcgtgct cagcaccacc agctatgtcg
      361 gcaccggcag cccgcgcttc tacctgccgc tggaccagat cttcccgcag accaacgtcg
      421 cccagatcgt cgtgctgccc agggatctga aggcgcgcga ggcattgagg ctgaagatcg
      481 tcgacgtctt caagaacgat tttccggaag tgcggggccg cgtgaagatg ctgcccaacg
      541 gaccgccggt gccgtacccg gtccagttcc gcgtgaccgg tccggaggtc gaggtggtgc
      601 gcgtgatcgc cgacgaggtc aaggaagtca tgcgcgcgaa cccgaacgcg gtcggcgtga
      661 acgacaactg gaacgagtcg gtcaaggtgc tgcgcctgga cctcgaccag gacaggctgc
      721 gcgcgctcgg cgtcagctcc cagtcggtga tgcgcactgc gaacaccatc ctgagcggca
      781 gcccgatcgg ccagttccgc gaccatgaca agctgatcga tatcgtcgtg cgccagccgg
      841 tcgaggagcg ttcgacgctc aacgcgctgg acaatgccta cattccgacc acgaacggcc
      901 gctcggtgcc gatgtcgcag gtggcacgcg ccggcttcgt ctgggagccg ggcgtggtct
      961 ggcgcgaggg gcgtgaatgg gcgatcaccg tgcagtcgga tgtggtggac ggcatccagg
     1021 ggccgaccgt ctccagccag atcgatccgc agctgaacaa gctgcgtgcg gtgctgccgc
     1081 cggggtaccg gatcgagttg gcgggggcgg ccgccgacag cggcaaggcg caggagtcga
     1141 tcgccgccaa catccccttg gtgatcttca tcatcttcac gctgctgatg ctgcagctgc
     1201 agagcttctc gcgctcggtg ctggtgttcc tgaccgggcc gctggggatt gccggcgccg
     1261 cgatggccct gctgctgctg cagcgaccat tcggcttcgt cgcccagctc ggcgtgatcg
     1321 cgctgttcgg catgatcgtg cgcaattcgg tgatcctggt cgaccagatc gagcaggaca
     1381 tccgcaacgg cgcggcgccc tgggacgcga tcgtggaggc ggcggtgcgg cgctgccgcc
     1441 cgatcgtgct gaccgccgcg gcggcggtgc tggcgatgat tccgctgtcg cgctcggtgt
     1501 tctgggggcc gatggccgtg gccatcatgg gcggcctgat cgtcgcgacc gcgctgaccc
     1561 tgctgttcct gcccgcgttg tacgcgacat ggttccgggt gaagaagccc gatccggcgc
     1621 tgcagacgat cccctgaaca gcgccgcgcc ggccgccgat ccggcgttcg cccccgaaaa
     1681 tcggggcatc ggcaataaaa atttagtaga attgcgggct tgaagttgaa gagctaccaa
     1741 caagagcttc gaatgggcga ccggattgac ttcaaggtcg ccctttgctt ttgtgcacgg
     1801 cacggttcgt aaggccccga aatcccgcga ggatggcgaa attggtagac gcaccaggtt
     1861 taggtcctga cgccagcaat ggtgtggggg ttcgagtccc cctcctcgca ccacacaaac
     1921 aatcaatctc aattcttgga cgattcacat ggctactgca gtcgaaaccc tggacaaact
     1981 cgaacgccgc atgacgatca ccgttccgct ggctgacgtg caagcggaag tggagaagcg
     2041 cctgaagatt cgcgccaaaa cggcgaaggc gcccggtttc cgtcccggca aggttccgat
     2101 gaagatggtg gcccagcagt acggctatca ggtcgagacc gaagttttga acgacaaggt
     2161 cggccgtgca ttctccgaag ccgccagcga aaacaacctg cgcgtggccg gcttccccaa
     2221 gatcgagccg aagaccggcg acggcgtggc cgaaggcatg gtcgcctttg atgccacctt
     2281 cgaggtctac ccggaagtga aggtcggcga cctctcctcc gccgacgtgg agaaagtgac
     2341 ctccgccgtc accgacgccg agatcgacaa gaccatcgac atcctgcgca agcagcgtgt
     2401 ccactatcac accaagggcg agcacggcgc gcatggcgac ggcggcgccg acacctcggc
     2461 ccagactggc gaccgcgtca ccctggactt cgtcggcaag atcgacggcg tcgaattcca
     2521 gggcggcaag gctgaaggct tttcgttcgt gctgggcgag
//
LOCUS       DATLGF010000416         4223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_10094,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000416 DATLGF010000000
VERSION     DATLGF010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10094"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VM406_12735"
     CDS             <1..377
                     /locus_tag="VM406_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HVL76873.1"
                     /translation="RLTSKVQRTFDRTLAILAEDMQKSGVAQVELDPPLEGKRWPAGL
                     EGTWHHMGTTRMHDSPKQGVVDRNCRVHGMNNLYIAGSSVFPTAGANFPTITLAALTF
                     RLSEHLVEELRNANSPLEMAAA"
     gene            427..1467
                     /gene="galE"
                     /locus_tag="VM406_12740"
     CDS             427..1467
                     /gene="galE"
                     /locus_tag="VM406_12740"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HVL76874.1"
                     /translation="MTHKTILVTGAAGYIGSHTCVELLAAGYAVVALDNLCNSSTESL
                     RRVERISGQVIPFYECDVRERASLDAILRAHRIDACIHFAGLKAVGESVEQPLAYFEN
                     NVGGSVTLLQALGEAGIKRFLFSSSATVYGDPESVPIAETARLSVTNPYGRSKLMVEQ
                     VLADLARSDAQWAIGVLRYFNPAGAHESGLIGEDPRGIPNNLMPYIAQVAVGRRERLS
                     VFGNDYPTPDGTGVRDYIHVVDLARGHVAALDALFRTGQGFTVNLGTGRGYSVLEMVR
                     AFEEASGRRVPYQITERRPGDIATCYADPQLALSLLNWRAQKGLADMCADHWRWQSSN
                     PQGYGESAGMRS"
     gene            1468..2430
                     /locus_tag="VM406_12745"
     CDS             1468..2430
                     /locus_tag="VM406_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HVL76875.1"
                     /translation="MPMRSVGKPIRILTAVLVLCAGAVAVVSACRAEGWLREHRVPAE
                     GPPRSPATPFAARLPYGQDPDGWVLTFSEEFERFDRSVWNDHIWYEKSNPTINYAVED
                     GLLKIWPQRDATGQFFNRTIDTDGKFYQTYGYFEMEAKLPRGKGTWPAFWLFNHIGDR
                     RPEIDIMEAYPGGVAPWGYTDRDGVRRPTAYGVTVWFDEDIKAGGLQYDVKRDLSRGF
                     HKYAVKWEPEKQTFYFDGKKVYEVNARMPDPKYIVLDLWFGSSSGEPDHTTPQGKSNS
                     FEINYVRAWQFRPDTGKAPHDTKPRGEVPHDAKPGGKEPRGKKD"
     gene            2925..3854
                     /locus_tag="VM406_12750"
     CDS             2925..3854
                     /locus_tag="VM406_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HVL76876.1"
                     /translation="MRSALLNRTTTTTAAVICAAALLAACGSGGVEDSLHSDTTVVTS
                     PDGSNSNADAGTPSSNPPSSAQPTQPAADIPGPYGQDANQYRLTFSEEFNNGFNRSVW
                     NDTIWYEQSNPTINYAVEDGKLKIWPQRDASGRFFNRTIDTDGKYYQTYGYFEIDAKL
                     PKGKGTWPAFWLFNHIGSRRPEIDVMEAYPGGAAPWGFTDSEGIRRPQAYAPTVWKGD
                     VEGQMMGSRQYDTGMDLSETFNKYAVKWEPNKQTFYFNGREILTVNVTMGDPMYLMLD
                     LWFGSASGTPDHMTPQGKNNSFEIKYVRAWQFR"
BASE COUNT          837 a         1333 c         1271 g          782 t
ORIGIN      
        1 ggcggctgac cagcaaggtg cagcgcacct tcgaccgcac gcttgccatt cttgccgaag
       61 acatgcagaa gagcggagtc gcgcaggtgg agcttgatcc accgctggaa ggcaaacgct
      121 ggccggccgg tctggagggc acctggcacc acatgggcac cacgcgcatg cacgattctc
      181 cgaagcaggg cgtggtggac cgcaattgca gggtgcacgg catgaataat ctgtatattg
      241 ccggcagctc ggtgtttccg accgccggcg cgaacttccc gaccatcacg ctggccgcgc
      301 tgacgttccg cctttccgag catcttgtcg aggagctgcg caacgcgaac agccctttgg
      361 aaatggcggc ggcctgaatc cgcagcagca gaagccggca agggaggccg gcaggaacca
      421 gaaatcatga cgcacaagac catactcgtg accggcgcgg ccggctatat cggctcgcat
      481 acctgcgtgg agctgcttgc agcaggctat gcggtggtgg cgctggacaa cctctgcaac
      541 agttcgaccg agtcgttgcg ccgcgtggag cgcatcagcg gccaggtcat tccgttctat
      601 gaatgcgacg tgcgcgagcg cgcgtcgctc gacgccattc tgcgcgcgca tcgcatcgac
      661 gcctgcattc acttcgccgg cctcaaggcg gtcggcgaat cggtcgaaca gccgctcgcc
      721 tatttcgaga acaacgtggg cggcagcgtc acgctgctgc aggcgctcgg cgaggccggc
      781 atcaagcgct tcctgttcag ctcgtcggcc accgtgtacg gcgatccgga gtcggtgccg
      841 atcgcggaga cggctcgcct gtcggtgacc aatccctacg gccgctccaa gctgatggtc
      901 gaacaggtgc tggccgatct ggcgcgctcg gacgcccaat gggcgatcgg cgtcctgcgc
      961 tacttcaatc cggctggcgc gcatgagagc gggctgatcg gcgaggaccc gcgcggcata
     1021 ccgaacaacc tgatgcccta catcgctcag gtggccgttg gccgccgcga gaggctttcc
     1081 gtgttcggca acgactatcc gacgcccgac ggcaccggcg tgcgcgacta catccatgtg
     1141 gtggaccttg cgcgcggcca tgtggcggcg ctcgatgcgc tgtttcgcac cggccagggc
     1201 ttcaccgtca atctcggcac cgggcgcggc tacagcgtgc tggagatggt gcgcgcgttc
     1261 gaagaagcaa gcggacgccg cgtgccctac cagatcaccg agcgccgtcc cggcgacatc
     1321 gccacctgct acgccgatcc gcagctggct ctctcgctgc tgaactggcg cgcgcaaaag
     1381 ggcctggccg acatgtgcgc cgatcattgg cgctggcaaa gcagcaatcc acagggatat
     1441 ggcgaatccg ccggcatgcg cagttgaatg ccgatgcgca gcgtgggcaa accgatccgg
     1501 atcctgacgg ctgtgctggt gctgtgcgcc ggcgcggtag cggtggtttc cgcctgccgg
     1561 gcagaggggt ggctgcgcga gcaccgggtg cccgccgaag gccccccgcg cagtcccgct
     1621 acgccgttcg cggcgcgcct gccatacggc caggatcctg acggctgggt gctgaccttc
     1681 tccgaagagt tcgaacgctt cgatcgcagc gtctggaacg accacatctg gtacgagaaa
     1741 tcgaacccga ccatcaacta tgcggtcgag gacggcctgc ttaagatctg gccgcagcgc
     1801 gacgcgaccg gccagttctt caaccgcacg atcgacaccg acggcaagtt ctaccagacc
     1861 tacggctact tcgagatgga ggcgaagctc ccccgcggga aaggcacgtg gcctgcgttc
     1921 tggttgttca atcacatcgg cgacaggcgg cccgagattg acatcatgga ggcctatccg
     1981 gggggcgtcg cgccctgggg ctataccgac cgggacggcg tgcgccggcc caccgcctat
     2041 ggcgtgaccg tgtggttcga cgaggacatc aaggccggcg gcctgcaata cgatgtcaag
     2101 cgcgatctgt cacggggctt tcacaagtat gcggtgaagt gggagccgga aaagcagacc
     2161 ttctatttcg acggcaagaa ggtgtatgag gtcaacgcgc gcatgccgga cccgaagtac
     2221 atcgtgctcg acctctggtt cggcagttcc agtggagagc cggaccatac gaccccgcag
     2281 ggcaaatcca attcgttcga gatcaactac gtgcgcgcct ggcagttccg gcccgatacc
     2341 gggaaagcgc cgcatgacac gaagccccgc ggcgaagtgc cgcatgacgc gaagcccggt
     2401 ggcaaagagc cgcgcgggaa aaaagactga accgttgcgc acttggctcg ccacggcaaa
     2461 attcccgacc gcatgcgatc cgctccccac cttgtcgtcc cgaacgcagt gagggatcgc
     2521 atccgcttgt cacgagacag cgtgttgcga accgatgcgg ttcctcgctg cgctcggaac
     2581 gacaggaagg ggacgtcatt cgctgcgggg gcgtgtcttc ccaaacgcat tgagggatcg
     2641 catccctgag tccggcatgg gctccttcaa gtcgcaattt gaatgaatcc ttcagtcccc
     2701 ctgccattca agctattcaa gcttgcaatt tcaaaccgaa aagccgctta aaacatttgc
     2761 ttacgtaaac ttttgtagca aagtttctga tccagatctc gttctttccc ctcggaaaaa
     2821 actgaagttg ccgcggaaca tttttccacc cggcaagctc tgactgctca cttcagtcgt
     2881 tgagctgaat caacttcact ttgttttttt gaaaggggac cgaattgaga tcggcactgc
     2941 ttaaccgtac gaccaccacc accgccgcag tcatctgtgc tgccgccctc cttgccgctt
     3001 gcggctcggg cggcgtggaa gactcgcttc actccgatac gaccgtcgtg acctctcccg
     3061 atggatcgaa cagcaatgct gatgccggca cgccgagcag caatcctccg tcgagtgccc
     3121 agccgaccca gcctgccgcg gatattcccg gaccgtacgg ccaggatgcc aatcagtatc
     3181 gcctgacctt ctctgaagaa ttcaacaacg gcttcaaccg cagcgtgtgg aacgacacga
     3241 tctggtatga acagagcaac ccgaccatca actatgcggt ggaagacggc aagctgaaga
     3301 tctggccgca gcgcgatgca tcaggccgct tcttcaaccg cacgatcgac accgacggca
     3361 agtactacca gacctacggc tacttcgaga tcgatgccaa gctgccaaag ggcaagggca
     3421 cctggccggc gttctggctg ttcaatcaca tcggcagccg ccgccccgag atcgacgtga
     3481 tggaagccta tccgggcggc gccgcaccgt ggggcttcac cgattccgaa ggcatccgcc
     3541 gtccgcaggc ctatgcgccg acggtctgga agggcgacgt cgaaggccag atgatgggct
     3601 cgcgccagta cgacaccggc atggacctgt cggagacctt caacaagtac gcggtcaagt
     3661 gggagccgaa caagcagacg ttctacttca atggccggga aatcctcacc gtcaatgtaa
     3721 cgatgggcga tccgatgtac ctgatgctcg acctgtggtt cggcagcgcc agcggcacgc
     3781 ccgatcacat gaccccgcag ggcaagaaca actcgtttga aatcaagtac gtgcgcgcct
     3841 ggcagttccg ctgatccgct tgcacgtttc ccaacaaagg gccggcttca tgccggcctt
     3901 ttttgttttg tctcttgcaa gaaattgttt tgaggcagtc ttctagcaca tcggagctgt
     3961 tggcaaagcg ttcttaagcg caataaaagt ccctcttttt tgctgattgg cggaaccatt
     4021 ttccgcgcgg aaaactctga tccacctctt gctgatgagc atcgttggct cccgtcaatc
     4081 cgaaagaaaa aggacctgat ttgtttacca tcaacgcccc ccgtatcgcc aagaccgctg
     4141 tttgctgtgc tgcttccgcc ctgttgctgg cttgcggagg aggctccggc atggatgaag
     4201 cgctgttctc cagcgcctcc ggc
//
LOCUS       DATLGF010000417         4998 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_172902, whole genome shotgun sequence.
ACCESSION   DATLGF010000417 DATLGF010000000
VERSION     DATLGF010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172902"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VM406_12755"
     CDS             complement(<1..188)
                     /locus_tag="VM406_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76877.1"
                     /translation="MPTTIVHRRINIVTLGPEESIAAHCAPDDIALVQEGSGWWVHFV
                     GEDGTSDTYDEPFDSYNKA"
     gene            complement(202..966)
                     /locus_tag="VM406_12760"
     CDS             complement(202..966)
                     /locus_tag="VM406_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family zinc transporter"
                     /protein_id="HVL76878.1"
                     /translation="MSTLPVWLQAGIWGLVAGSALVIGAAIGYFTRIRHRVIAAIMAF
                     GSGVLISALSFDLVEEAHERGGFLASAVGFVAGASIFTVANWYLSYRGAAARNLSGQQ
                     QPSEEEHHGSGAAIALGALIDGIPEAIVIGVSMIASKSVSMVAVIAVFLSNIPEGLSS
                     ASGMKKAGRSAFYVFGVWGGIAIMNGIAAALGNLLFVGFSEQVIAATMALAAGAILAM
                     LIDTMIPEAFEVAQNYAGMIAVAGFLAAFYLSKLSG"
     gene            complement(1074..1520)
                     /locus_tag="VM406_12765"
     CDS             complement(1074..1520)
                     /locus_tag="VM406_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PACE efflux transporter"
                     /protein_id="HVL76879.1"
                     /translation="MQGIKRKIVYVTAYELIAVTVCSGGFVAFSDASLQMAGALSVTT
                     SLIAALWNFVFNMLFEAWESRQARRGRSLLRRLVHAVAFEAVLFAMFVPLIAWWLDVT
                     LLHALAMNTGLAVFFLVYTFVFAWCFDRIFGLPASAQALGSTAPAR"
     gene            complement(1617..2498)
                     /locus_tag="VM406_12770"
     CDS             complement(1617..2498)
                     /locus_tag="VM406_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL76880.1"
                     /translation="MNARTQYRLSSADLELLLALVRGGTLAAAGSRLGVDGSTVFRGL
                     QRIERGLGKALFERTRSGYHAGELALELAQHAERLEAELEAARSRFQTTPEQVAGQVR
                     ITTTDTILHGLVAPALGSLRERHPLLEFELETSNELANLSRRDADIAVRAAHNPPEHL
                     VGRSLGPIRVALFAPRKSRIRSLDEAVSSAAWIAPDDALPDHPSVRWRRRHFPKITPV
                     YRVSSILSAMECVELGLGVAVLPLFLAGPRRGVRQISDEIEESTTQLWLLSHPEGRYL
                     RRVSTALEHLAGRIRLE"
     gene            2576..3040
                     /locus_tag="VM406_12775"
     CDS             2576..3040
                     /locus_tag="VM406_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HVL76881.1"
                     /translation="MKSTAIDSAHRPSNAHSWRVGSSAAHGITLLRVSLGVMWIAHAL
                     LKPIVFTLPGTAQFFDSVGIPGWMAYPVFAVELLGGIALLLGIYARQASLLLVPVMLV
                     ATWVHIPNGWLHTSAGGGWEYPAFLVSASLVLWLAGDGSFTLRTSSRFTPDR"
     gene            3055..3735
                     /locus_tag="VM406_12780"
     CDS             3055..3735
                     /locus_tag="VM406_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVL76882.1"
                     /translation="MHPTFTLISHPLCPYVQRAAIVLREKGVAFTRREIELADKPNWF
                     LAISPLGKTPVLLVDETPIFESMVICEYLDETVGERLHPHNALLRARHRAWIEFGSSI
                     LNVIGSFYSVHDEAALQRAKAEIRTKFVLLEAALAEGGPFFEAERFSMIDAVYGPIFR
                     YFATFERIGESGFFDGLEKLAGWRTALLTRASVRDAVRADYGDLLLAFLKARNSALSS
                     RILSVGAG"
     gene            complement(3832..>4998)
                     /locus_tag="VM406_12785"
     CDS             complement(3832..>4998)
                     /locus_tag="VM406_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HVL76883.1"
                     /translation="DVAVIDEIQMLGDRERGAAWTAAVCGAPANVVYLVGAVEARRAI
                     EALARRLDCPLEVRILKRKAPLTMEAQPVRKLRNLRRGDAVIAFSRRDVLMWRDLITE
                     AGMSVATVYGNLSPEVRRAQAARFRDGEADIVVGTDAIAMGLNLPIRRVVMTAVSKFN
                     GVEEEELSAALARQIAGRAGRFGVHEEGFVAGHDDEAHYVMRALLQEKVPPVPAQGFA
                     VAPTLEHLNRIAAVTGEQSLAKLLKRFIHNIDVPDGFFTPRITEEQQDRALWLDTLPL
                     SLEDKFTLSLVPISTRIETLHLAWQRWARALARRHPFILQHRDNHLAGQNLQEVEDSC
                     KLYSAYAWLGYRCPAFFPDGELARQLARDASDRVDAILQRQNAALRRQRGRRAA"
BASE COUNT          900 a         1675 c         1527 g          896 t
ORIGIN      
        1 gccttgttgt agctgtcgaa cggctcgtcg taggtgtcgc tggtcccgtc ttctccgacg
       61 aaatgcaccc accagccgct cccttcctgg accagcgcaa tgtcgtccgg cgcgcagtgc
      121 gcggcaatgc tttcttccgg cccgagcgtg acgatgttga tgcggcgatg aacgatggtc
      181 gtaggcatgt cccattcttt cctaaccact gagcttggag agataaaacg cggcaagaaa
      241 ccctgcaacc gcgatcatcc cagcgtaatt ctgcgcgacc tcgaacgcct cggggatcat
      301 cgtatcgatc agcatggcga ggatggcgcc tgccgccaaa gccatcgtgg cggcaatgac
      361 ctgttcggaa aatccgacaa agagaaggtt cccgagcgcc gccgcgattc cgttcatgat
      421 tgcgatgccg ccccacacgc caaagacata gaacgccgag cgtccggcct tcttcatgcc
      481 cgacgcgctc gacaagccct cagggatgtt ggacaggaag accgctatca ccgccaccat
      541 gctgacggac ttgcttgcga tcatgctcac cccgatgaca atcgcttccg gaataccatc
      601 gatgagcgcc cccagcgcaa ttgcggcgcc gctgccatgg tgctcttctt ccgagggctg
      661 ctgctgaccc gacagattgc gcgccgccgc gccgcggtag gacagatacc agttcgccac
      721 cgtaaaaatg gaagcgccgg caacgaagcc cacggcagag gcgaggaagc cgccgcgctc
      781 gtgggcttcc tccaccaggt cgaacgacag cgcggagatc agcaccccgc tgccgaacgc
      841 catgatcgcg gcgatcaccc gatgtctgat gcgggtgaag tacccgatcg ccgccccgat
      901 caccagcgca gatcccgcca ccaggcccca aattccggcc tgcagccaaa caggcaatgt
      961 cgacatgatg ttctcccgcc tgctgcatgg acagcaaaca gtgcggaaat gctcacgccg
     1021 ggcaggaaac gggaagccgc tcaagccgga cgctggcgtc cggcgacggc agcttagcgg
     1081 gcaggcgcgg tcgaaccaag cgcctgtgcg gaagcgggca ggccgaatat ccggtcgaag
     1141 caccaggcga acacgaaggt atagaccagg aagaacacgg caagtccggt attcatcgcc
     1201 agcgcgtgga gcagggtcac atccagccac caggcgatca gcggtacgaa catcgcaaac
     1261 agcaccgctt cgaaagctac tgcatgcacg aggcgtcgca gcaggctgcg cccgcgccgc
     1321 gcttgccgcg actcccaggc ctcgaacagc atgttgaaga cgaagttcca aagtgccgca
     1381 atcagcgagg tcgtcaccga cagcgcgcct gccatttgga gacttgcatc cgaaaatgcg
     1441 acgaacccgc cgctgcacac cgtcaccgcg atgagctcat aggcggtgac gtacacgatc
     1501 ttgcgcttga ttccctgcac gactgctttc gtttttgaac ggaagggcac attctagtgg
     1561 ctcttgccga agtgcgcgtt gcatccgatg aacacggatc cgcaactcga atacccctat
     1621 tcgagcctga tgcgccccgc cagatgctcc agcgccgtgg acacccgcct caggtaccgc
     1681 ccttccggat ggctcagcaa ccacagctgg gtcgtacttt cttcgatttc gtcactgatc
     1741 tgccgcacgc cgcgccgcgg gccggccaga aacagcggga gcaccgccac cccgagcccg
     1801 agctcgacgc actccatggc cgaaagaatg ctgctcacgc ggtagaccgg cgtgatcttc
     1861 gggaaatgcc ggcggcgcca gcgtacggaa ggatgatcgg gcagggcgtc gtcgggcgct
     1921 atccatgccg ccgaactgac cgcctcgtcc agcgagcgga ttcggctttt tcggggcgca
     1981 aacagggcta cgcgaatggg cccgagagac cggccgacga ggtgctcggg tggattgtgc
     2041 gccgcgcgca ccgcaatgtc cgcgtcgcgc cggctcaggt tcgccagctc gttgctggtt
     2101 tcaagctcga actccagcag cggatgccgc tcccgcagcg agcccagcgc cggcgccacc
     2161 aggccgtgca ggatggtgtc cgtggtggtg atgcgcacct gtccggccac ctgttccggc
     2221 gtggtctgga agcgcgaccg tgccgcctcc agctcggctt cgagccgttc ggcgtgctgc
     2281 gccagctcga gtgcgagctc gccggcgtga tagccggagc gtgtgcgttc gaacagggct
     2341 ttccccaatc cgcgttctat ccgctgcagg ccgcgaaaca ccgtcgatcc gtccacgccg
     2401 agccgcgagc ctgccgctgc cagcgtaccg ccgcgcacga gcgcgagcag cagctccagg
     2461 tcggcactgc tgagtctgta ttgcgttctt gcattcatgg attgcagttt cgcttattta
     2521 cgctgcgatt ttgcaatcat agactgggct ctccaactca caaaggagaa cttccatgaa
     2581 gtccaccgcg atcgattccg cgcaccgtcc gtcgaatgcg cattcctggc gtgtcgggtc
     2641 ctcggccgcc cacggcatca ccttgctgcg ggtgagcctg ggtgtgatgt ggatagccca
     2701 tgcgctcctc aagcccatcg tctttacctt gcccggcacc gcgcagtttt tcgacagcgt
     2761 cggcattccg ggctggatgg cttatccggt ctttgccgtt gaactgctgg gtgggatcgc
     2821 acttctgctc ggcatctacg cacgccaggc ctcgctgctg ctggtgccgg tgatgctcgt
     2881 cgcgacctgg gttcacatcc cgaatggatg gctgcacacc agcgctgggg gaggctggga
     2941 atacccggcg ttcctggtat cggcttcgct cgtgctctgg ctggccggcg acggttcatt
     3001 cacgctgcgc accagcagcc gcttcacgcc tgacaggtaa gcgaggagag caacatgcat
     3061 ccgacattca ccctgatcag tcatccactc tgcccgtacg tccagcgcgc cgccatcgtg
     3121 ctgcgtgaga agggcgtggc gttcacacgc cgcgagatcg aacttgccga caagcccaac
     3181 tggtttctcg ccatctctcc gcttggcaag acgcctgtgc tgctggtgga cgagacgccg
     3241 atcttcgagt cgatggtgat ctgcgaatac ctggacgaaa cggtcggcga gcgcctgcac
     3301 ccgcacaacg cgctcctgcg tgcgcggcac cgcgcgtgga tagagttcgg ttcgtcgatc
     3361 ctgaacgtga tcggcagctt ctactccgta cacgacgagg cggcactgca gcgggcgaaa
     3421 gcggagatcc gcaccaagtt cgtgctgctg gaggccgcgc tcgccgaggg cgggccgttc
     3481 tttgaagccg aacgcttctc gatgatcgac gcggtgtacg gcccgatctt tcgctacttc
     3541 gcgaccttcg agcgcattgg cgaatccgga ttcttcgacg gcctcgaaaa gcttgccggc
     3601 tggcgaacgg cactgctcac gcgtgcgtcg gtgcgcgatg cggttcgcgc cgattacggc
     3661 gacctgctgc tcgcgttcct gaaagccaga aactcggcgc tctcaagccg gatcttgagc
     3721 gtgggcgccg ggtgagacgc ccgcttgcac cggagcccgg ccggcagacg ttgtcgatgg
     3781 cgccagcgtc tgcacggcac ggccgctgcg ttccagcgtg cgctgacgtg ctcaggcggc
     3841 gcgccgcccc cgctgccggc gcagtgccgc attctgcctc tgcagaatcg catctacgcg
     3901 gtccgacgcg tctctcgcca gttgccgggc cagttccccg tccgggaaga acgcgggaca
     3961 acggtagccg agccaggcat aggccgaata cagcttgcag ctgtcctcga cctcctgcag
     4021 gttttgcccg gcaagatggt tgtcgcggtg ctgaaggatg aaaggatgtc gccgtgccag
     4081 tgcccgcgcc catctctgcc atgccagatg aagcgtctca atgcgcgtcg agatcggcac
     4141 cagcgacagc gtgaacttgt cttccagcga aagtggcaag gtatccagcc agagcgcgcg
     4201 gtcctgctgt tcttcggtga tgcgcggcgt aaaaaagccg tccggcacat cgatgttgtg
     4261 gatgaagcgc ttgagcagct tcgccagcga ttgctcgccg gtcaccgccg cgatgcggtt
     4321 caggtgttcc agcgtgggcg caacggcgaa tccttgcgcg ggcacgggcg gcaccttttc
     4381 ctgcaacagc gcgcgcatca cgtaatgcgc ttcatcgtca tggccggcca caaaaccttc
     4441 ttcgtgcaca ccgaagcgcc cggcgcgccc cgcgatctgc cttgccagcg cggcggatag
     4501 ctcctcttcc tcaacgccgt tgaacttgct caccgccgtc atcaccacgc gccggatcgg
     4561 caggttcaag cccatcgcga tcgcatcggt gccgaccacg atatcggctt caccgtcccg
     4621 aaagcgtgca gcctgagcgc gtcgcacttc cggcgacaga ttgccgtaga cggttgcaac
     4681 cgacatgccg gcttcggtga tcagatcgcg ccacatcaac acatcgcgtc gcgagaacgc
     4741 gatgacggcg tcgccgcgtc gcagattgcg cagcttgcgc accggttgcg cctccatcgt
     4801 caaaggcgcc ttgcgcttga gtatgcgcac ttcgagcggg cagtccaggc gcctggccag
     4861 cgcttcgatc gcgcgccgtg cttcgaccgc gccgaccagg tagacgacat tcgccggcgc
     4921 cccgcacacg gcggcggtcc aggctgcgcc gcgctcgcgg tcgcccagca tctggatctc
     4981 gtcgatcacg gcgacatc
//
LOCUS       DATLGF010000418         6844 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_172903, whole genome shotgun sequence.
ACCESSION   DATLGF010000418 DATLGF010000000
VERSION     DATLGF010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172903"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VM406_12790"
     CDS             complement(<1..824)
                     /locus_tag="VM406_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HVL76884.1"
                     /translation="MQIQSMHFKERAGQKLADQRLQQNLKKLSTKFVSGRASAILELD
                     DFEGTRDAAIERRMRAIRNLDVWLETFEQEATRRGATVLYAESADDASRLVVEIAQKH
                     GVKKAIKTKSMVSEEMALNKALEAVGVHPIETDLGEYILQINNNEAPSHIIAPVVHKD
                     KEEISDLFAKVHQRPRLTDIPQMTREAREVLRPHFLSADMGITGGNFIIAETGSVAVV
                     TNEGNEGMCTIMPPKVHVVVTGIEKVLPTLEDFATVMRLLPRSATGQSISNYVSLLT"
     gene            complement(824..1549)
                     /locus_tag="VM406_12795"
     CDS             complement(824..1549)
                     /locus_tag="VM406_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HVL76885.1"
                     /translation="MRVGLFVTCLVDAMRPEIGFSALKLLEGAGCTVEVPALQTCCGQ
                     PAYSSGDRAAARALAEKFLREFEQFDYVVLPSGSCGGTVKVHYADLLADDPNLAARME
                     RLAGRVYELTDFLQNVLKLEALPQGSRPGCVTYHDSCSGLRELGVQAQPRALLAKAGV
                     EIREMTDSRQCCGFGGTFSIKYGDISSAIVDEKCANIHASGADAVVLGDLGCMLNIEG
                     RLRRTGDDRTRVLHVAQVLAGDC"
     gene            1715..2506
                     /locus_tag="VM406_12800"
     CDS             1715..2506
                     /locus_tag="VM406_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HVL76886.1"
                     /translation="MKQESVLENSQTSIQVIERMVSLLDALALHPDPVSLKDLSGMTG
                     LHPSTAHRILNDLVVKRFVDRAEAGSYRLGMRLLELGNIVKSRLNVREAALDFMRALH
                     RKTQQTVNLSVRQGDEIVYIDRAFSERSGMQVVRAIGGRAPLHLTSTGKLFLSVDDAK
                     LVRAYATRTGLAGHNKNSITDLARLERELSLVRARGYARDNEELELGVRCMAAGILDD
                     SGKLVAGLSISAPADRLQEEWLEDLVRTANEISYVLGYVREQVVG"
     gene            complement(2519..3127)
                     /locus_tag="VM406_12805"
     CDS             complement(2519..3127)
                     /locus_tag="VM406_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76887.1"
                     /translation="MAAPLADDAPAALADAQASLFEEPACLRALHEHAMPLLRTYPAP
                     RVWLADCAHAEDTLAIAALLREHRLLERTQVFVSAPRADALARIRSDRLSPRRLTACR
                     AAWRALGGEVEPDATHVRDGEFIWVEQALGAGASFSEFELIVCRRALCDFGPGLRARV
                     LHLFAESLPLLGMLYASADRLGGLGPRFAERADCPGLYRRVA"
     gene            complement(3112..>6844)
                     /locus_tag="VM406_12810"
     CDS             complement(3112..>6844)
                     /locus_tag="VM406_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL76888.1"
                     /translation="SQVRNIAEVTTAVANGDLSKKITVDVRGEILQLKDTMNTMVDQL
                     RSFASEVTRVAREVGTEGRLGGQANVPGASGTWRDLTDNVNFMASNLTGQVRNIAEVT
                     TAVARGDLSKKITVDVKGEMLELKNTINVMVDQLSSFASEVTRVAREVGTEGKLGGQA
                     QVEGAAGTWKDLTDNVNFMAANLTAQVRGIAGVVTAVANGDLKKKLTVAARGEIEALA
                     NTINEMTATLAVFADQATTVAREVGVEGKLGGQASVPGAAGTWKDLTENLNRMAATLT
                     TQVRAIAEVATAVTRGDLSRSIQVEARGEVAELKDNINEMIRNLKETTQKNAQQDWLK
                     TNLARFTRLLQGQRDLSAVTTLILSELAPLVSAHHGVFYMMDSQDQDARLRMIASYGY
                     RSSRKLPTSFLPGEGLVGQCAREKQRIWLTNVPRDYIQISSGLGAAPPTNIVVLPILF
                     EQQVKAVIELASLDRFTETHLWFLDQLMESIGVVLNTIEANSRTESLLTQSQSLAQEL
                     QQTNLELAEKARLLSEQNIEVERKNREVEQAKLALEEKATQLALSSKYKSEFLANMSH
                     ELRTPLNSLLILAQQLADNPEGNLSERQVEFAKTIYGSGSDLLTLINDILDLSKIESG
                     TVTLELSEYRFRTLSAYVERTFRHMAEAKQIAFSVDLTEDLPSSMMTDTTRLQQILKN
                     LLSNAFKFTAQGKVALRIGMVKSGWSPDHPNLSHAESVLAFSVTDTGVGIPSDKLQLI
                     FEAFQQADGSTARKYGGTGLGLSISRELARLLGGEIRVVSEVGVGSTFTLYLPYNQGG
                     FVHYDSLPEGGRAASVPTLDPFDSGEVSIIEAHASSDDLPATQGNGDDRAGTGSGDQA
                     VLIVESDAALAARLLQTARKRGFKGIVTQQGDSALTLARDYQPLAILLDLDMGKAMGV
                     TVLERLKRDPATRHIPVHALSAAGERENVLQKGAMSFLRKPVGDEQLEAAFADIQQFN
                     SRERRNLLVVEDDATQRESIVQLLAAHDVNIVAVGSGREALEALGESPFDCVVVDLSL
                     PDIDGFDLLSRIGSEPGLQDLPIVVYTARDLTRKEAAQLKRLAQTVVLKDVRSPERLL
                     DETALFLHRAPAGMSAPQRRMLEDMRAASSSLSGRKVLIVDDDLRNIFALSSVLERQH
                     MKVLFAENGKDGIEVLKKEPDIEIVLMDIMMPEMDGYDTMRAIRRIQRFKDLPIITLT
                     AKAMKGDRDKCLAAGASDYITKPVDVGQLLALMRVWLHR"
BASE COUNT         1135 a         2280 c         2158 g         1271 t
ORIGIN      
        1 gtgagcagcg acacatagtt ggaaatcgac tgcccggtgg ccgaacgcgg cagcagccgc
       61 atcacggtcg cgaagtcttc gagcgtgggc aatacctttt cgatgccggt gacgaccacg
      121 tgcaccttgg gcggcatgat cgtgcacatg ccttcattgc cctcgttggt caccaccgcc
      181 acggagccgg tctcggcgat gatgaagttg ccgccggtga tgcccatgtc ggccgacagg
      241 aagtgcgggc gcagcacctc gcgcgcctcc cgcgtcatct gcggaatatc ggtcaggcgc
      301 ggacgctggt gcaccttcgc gaacaggtcc gaaatctctt ccttgtcctt gtgcaccacc
      361 ggcgcgatga tgtgcgaagg cgcctcgttg ttgttgatct ggagaatgta ttcgccgaga
      421 tcggtttcga tcggatgcac gccgaccgcc tccagcgcct tgttcagcgc catctcctcg
      481 gagaccatcg atttcgtctt gatcgccttc ttcacgccgt gcttctgcgc gatctccacc
      541 accaggcgcg acgcatcgtc cgcgctttcg gcgtacagca cggtcgcgcc gcggcgcgtg
      601 gcctcctgct cgaaggtttc cagccacacg tcgaggttgc ggatcgcgcg catgcgccgc
      661 tcgatcgcgg catcgcgcgt gccttcgaag tcatccagtt ccaggatcgc cgacgcccgc
      721 ccgctgacga acttggtcga cagcttcttc aggttctgct gcagccgctg gtccgccagc
      781 ttctggcccg cgcgctcctt gaaatgcatc gattgaatct gcatcagcaa tctcccgcca
      841 gtacctgggc cacatgcagc acgcgcgtcc tgtcgtcgcc ggtacgccgc aaccgtccct
      901 cgatgttcag catgcagccg agatcgccga gcacgaccgc gtcggcgccg gacgcgtgga
      961 tgttcgcgca cttctcgtcg acgatggccg atgaaatgtc gccgtacttg atcgagaacg
     1021 tgccgccgaa gccgcagcac tgccggctgt cggtcatttc ccggatctcc acgccggcct
     1081 tggcgagcag cgcgcgcggc tgggcctgca ccccgagttc gcgcaatccg ctgcaggagt
     1141 cgtggtaggt cacgcagccg ggacggctgc cttgcggcag cgcctcgagc ttcagcacgt
     1201 tctgcaggaa atcggtcagc tcgtagaccc ggcctgccag gcgctccata cgtgccgcca
     1261 gatttgggtc gtcggcaagc aggtcggcgt agtgcacctt gaccgtgccc ccgcaggagc
     1321 cggacggcag tacgacatag tcgaactgtt cgaattcccg aaggaatttt tcagccagcg
     1381 cacgagcggc ggcacgatcg cccgagctgt acgctggctg gccgcagcag gtctgcagag
     1441 ccggcacttc gacagtgcat cccgcgcctt cgagcagttt gagcgcagag aagccgatct
     1501 cgggccgcat tgcatcgaca aggcaagtta cgaagagtcc tacacgcatg gccgcctttc
     1561 cgcgttcaac gatgacatgg cgccattatg caacccggtg gcccccgtgg aagcttgcct
     1621 gcagatgaaa aaaattgcgc agcagcgtcg agggtggctg ccgtggtttt cacaatgtgg
     1681 tataaaccgg aatattgcat tgcaccagac gcgcatgaaa caagaatccg tactcgaaaa
     1741 cagccagacc tccatccagg tgatcgagcg catggtgtcg ctgctcgatg ccctcgccct
     1801 gcaccccgac ccggtcagcc tgaaggacct gtccggcatg accggcctgc atccgtcgac
     1861 cgcgcaccgg atcctcaacg atctggtggt caagcgtttc gtcgaccgcg ccgaggcggg
     1921 ttcgtatcgc ctcggcatgc ggctgttgga actcggcaat atcgtaaaga gccggctgaa
     1981 tgtgcgcgaa gccgcgctgg atttcatgcg cgcgctgcac cggaagaccc agcagacggt
     2041 aaacctctcc gtgcgccagg gcgacgaaat cgtctacatc gaccgcgcgt tttccgagcg
     2101 ttccggcatg caggtggtac gggcgatcgg cggacgcgcg ccgctgcacc tgacctccac
     2161 cggcaagctg tttctgtcgg tcgacgacgc caagctcgtg cgcgcctatg cgacccgcac
     2221 cggcctggcc ggccacaaca agaactcgat caccgacctc gcccgtctgg agcgcgaact
     2281 gagcctggtg cgcgcccgcg gctacgcgcg cgacaacgaa gagctggaac tcggcgtgcg
     2341 ctgcatggcg gccggcatcc tcgacgacag cggcaagctc gtggcgggcc tgtccatttc
     2401 ggcgccggcc gaccggctgc aggaagaatg gctggaggac ctcgtcagga cggccaacga
     2461 gatctcgtat gtgctcggct acgtgcgcga gcaggtggtc ggataagaac ggaagatttt
     2521 acgcgacgcg ccggtagagc ccggggcagt ccgcccgttc ggcaaagcgc gggccgagtc
     2581 ccccgaggcg atcggcgctc gcatacaaca tgcccaggag cggcaggctt tctgcaaaca
     2641 ggtgcagcac gcgcgcccgc agcccggggc cgaagtcaca cagcgcacgc ctgcagacga
     2701 tcagttcgaa ctcgctgaac gacgccccgg ctcccagcgc ctgctcgacc cagatgaatt
     2761 ccccatcgcg tacatgcgtg gcgtcgggct cgacctctcc tcccaacgca cgccacgccg
     2821 ccctgcaagc cgtgagccgc cgtggcgaaa gacggtcgga tcggatgcgc gcgagcgcgt
     2881 ccgcgcgggg agcgctgacg aacacctgag tgcgttccag caggcggtgt tcgcgcagca
     2941 acgcggcaat cgccagcgtg tcctccgcat gagcgcaatc ggccagccag acgcgcggcg
     3001 ccggataggt gcgcagaagc ggcatcgcgt gctcgtgcag cgcgcgcagg catgccggtt
     3061 cctcgaacag gcttgcctgc gcgtctgcca gcgccgcagg cgcatcgtcg gctagcggtg
     3121 cagccatacc cgcatcagcg ccagcaactg tccgacgtcc accggcttgg tgatgtagtc
     3181 cgacgccccg gcggccaggc acttgtcgcg gtcgcccttc atggctttcg ccgtgagcgt
     3241 gatgatcggc agatccttga aacgctggat gcggcggatc gcgcgcatcg tgtcgtagcc
     3301 atccatctcc ggcatcatga tgtccatcag cacgatctcg atgtcgggtt ccttcttcag
     3361 cacctcgatg ccgtccttgc cgttctctgc gaacagcacc ttcatatgct gtcgctcgag
     3421 caccgagctg agcgcgaaga tgttgcgcag gtcgtcgtcg acgatcagca ccttgcgccc
     3481 ggacagcgag ctgctggccg cgcgcatgtc ctccagcatg cgccgctgag gcgccgacat
     3541 gccggccggc gcgcgatgga ggaacaacgc cgtttcatcg agcaggcgtt ccggcgagcg
     3601 cacgtccttg agcaccaccg tctgtgccag ccgcttgagc tgggcggcct ccttacgcgt
     3661 gagatcgcgc gcggtataga caacgatcgg caggtcctgc agcccgggct cggacccgat
     3721 gcgcgagagc agatcgaagc cgtcgatgtc cggcaagctg aggtcgacca ccacgcagtc
     3781 gaacggcgac tcgccgagcg cttccagtgc ttcccgtccg ctaccgacgg caacgatgtt
     3841 cacgtcgtgc gcggccagca actggacgat ggattcgcgc tgggtcgcgt cgtcctccac
     3901 aaccagcagg ttgcgccgct cgcggctgtt gaactgctgg atgtcagcga acgccgcttc
     3961 cagctgctcg tcgcccacag gtttgcgcag gaaggacatc gcccccttct gcagcacgtt
     4021 ttcccgttcg ccggccgcac tcagtgcatg caccgggatg tgccgggtcg cagggtcgcg
     4081 cttgaggcgc tcaagcactg tcacgcccat ggccttcccc atatccaggt caagcaggat
     4141 cgccaatggc tggtagtcac gcgcaagtgt gagcgccgag tcgccctgct gggtgacgat
     4201 acccttgaat ccacgcttgc gcgccgtttg tagcagccgg gccgccagcg cggcgtccga
     4261 ttcgacgatc aacacagctt ggtcgccgga gccggtgccc gcgcggtcgt caccattgcc
     4321 ctgggtcgcc ggcaggtcgt cggacgaggc gtgcgcctcg atgatgctca cctcgccgga
     4381 atcgaacgga tcgagggttg gtacggaagc ggcccgcccg ccctcgggca gcgaatcata
     4441 gtgaacgaaa ccgccctggt tatatggcag gtagagcgtg aacgtgctgc ccacgccgac
     4501 ttcactgacg acccggatct cgccgcccaa gagacgcgcc agctcccggc tgatcgacag
     4561 gcccaggccg gtgccgccgt acttgcgtgc ggtactgcca tcggcctgct ggaaggcctc
     4621 gaagatcagc tgcagcttgt cggacggaat gccgacgccc gtatcggtga cggagaacgc
     4681 cagcaccgac tcggcatggc tgaggttcgg atgatccggg ctccagccgc tcttgaccat
     4741 gccaatgcgc agcgccacct tgccctgagc ggtgaacttg aacgcattcg acagcaggtt
     4801 cttgaggatc tgctggaggc gggtggtatc ggtcatcatc gaagatggca ggtcctcggt
     4861 cagatcgacc gagaatgcaa tctgcttggc ttccgccatg tggcggaatg tgcgctccac
     4921 gtaggccgac aaggtccgga agcggtactc ggacaactcc agcgtgacgg tgccggactc
     4981 gatcttggac aggtcgagaa tgtcgttgat cagcgtgagc aggtcgctgc cggagccata
     5041 gatggtcttc gcgaactcga cctggcgttc ggacaggttg ccttccgggt tgtcggccag
     5101 ttgctgcgca aggatcagca ggctattcaa cggcgtgcgc agctcgtgcg acatgttggc
     5161 gaggaactcg gacttgtact tcgacgagag cgccagctgc gtcgccttct cttccagcgc
     5221 gagcttggcc tgttcgactt cgcggttctt gcgctccacc tcgatgttct gctcggacag
     5281 caggcgcgcc ttttccgcca gttcgaggtt ggtctgctgc agctcctgcg ccagcgactg
     5341 cgactgggtc agcagagact ccgtccggct gttggcttcg atggtgttga gcacgacgcc
     5401 gatcgattcc atcagctggt cgaggaacca tagatgggtt tcagtgaagc ggtccagcga
     5461 tgcgagttcg atcaccgcct tgacctgctg ctcgaacagg atcgggagca ccacgatgtt
     5521 ggtcggcggc gcggcaccga ggccggagga aatctgtata tagtcgcgcg gcacgttggt
     5581 cagccagatg cgctgctttt cgcgcgcgca ctgcccgacc agcccttcac ccggcaggaa
     5641 ggaagtcggc agcttgcggc tcgagcgata gccgtagctg gcgatcatgc gcagccgcgc
     5701 atcctggtcc tgcgagtcca tcatgtagaa cacgccgtgg tgggccgata ccagcggcgc
     5761 cagttcggac aggatcagcg tggttacggc ggacaggtcg cgctggcctt gcaacagacg
     5821 cgtgaagcgc gcgaggttgg tcttcagcca gtcctgctga gcgttcttct gcgtggtctc
     5881 cttcaggttg cggatcatct cgttgatgtt gtccttgagc tccgccacct cgccgcgtgc
     5941 ctccacctgg atcgagcgcg acaggtcacc gcgcgtcacc gcggtcgcca cttccgcgat
     6001 cgcccgcacc tgggtggtga gcgtggccgc catgcggttc aggttctcgg tcaggtcctt
     6061 ccacgtgccg gcggcgcctg gcaccgaggc ctggccgccg agcttgccct ccacgcccac
     6121 ttcgcgcgcc accgtggtcg cctggtcggc gaacaccgcc agcgtcgccg tcatttcatt
     6181 gatggtgttg gccagcgctt cgatctcgcc gcgcgcggcc acggtcagct tcttcttcag
     6241 gtcgccgttt gccacagcgg tgacgacgcc ggcgatcccg cgcacctggg cggtcaggtt
     6301 ggccgccatg aagttcacgt tgtcggtcag gtccttccag gtaccggccg ctccttccac
     6361 ctgtgcctgg ccgccgagct tgccctcggt gccgacttca cgcgccacgc gcgtcacttc
     6421 ggatgcgaac gatgagagct ggtccaccat cacgttgatt gtgttcttca gctccagcat
     6481 ctcgcccttg acgtcgacgg tgatcttctt cgacagatcg ccgcgcgcca ccgcggtggt
     6541 cacttccgcg atgttgcgca cctgtccggt caggttcgag gccatgaagt tgacgttgtc
     6601 ggtcaggtcg cgccaggtgc cggacgcccc tggcacgttc gcctgcccgc ccagccggcc
     6661 ctcggtgccc acctcgcgcg cgacgcgtgt cacttcggat gcgaacgagc gaagctggtc
     6721 gaccatcgtg ttcatcgtgt ccttgagctg caggatctcg ccgcgcacgt ccaccgtgat
     6781 cttcttggac aggtcgccgt tcgcgaccgc agtcgtcacc tccgcgatgt tgcgcacctg
     6841 cgag
//
LOCUS       DATLGF010000419         2516 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_10213,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000419 DATLGF010000000
VERSION     DATLGF010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10213"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VM406_12815"
     CDS             complement(<1..292)
                     /locus_tag="VM406_12815"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL76889.1"
                     /translation="MKRTSCPWPTAPYPLVPQQPVVAKHARQHLTRSHISLKQWNTLL
                     AVVHHGSFARAAEVLHISQPAISYTIAKLEERLGISILEPDGRRAAITDAGLK"
     gene            complement(716..940)
                     /locus_tag="VM406_12820"
     CDS             complement(716..940)
                     /locus_tag="VM406_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76890.1"
                     /translation="MIDSIACVSATPCATKVSISRYARQALLLPGISIPFKDMVVRKR
                     VGHLVEINILMTVARKLQALLPDSVVRMGR"
     gene            939..1256
                     /locus_tag="VM406_12825"
     CDS             939..1256
                     /locus_tag="VM406_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVL76891.1"
                     /translation="MQRVIAAAVALTLSGAVAPHGALASQELARSKGCVACHAMDKKL
                     VGPAFKDVAARYADERGAEDRLVEKVLKGSQGAWGAIPMPANTNVSQEEARALVRWIL
                     QGG"
     gene            complement(1315..2013)
                     /locus_tag="VM406_12830"
     CDS             complement(1315..2013)
                     /locus_tag="VM406_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HVL76892.1"
                     /translation="MLGRIFTEQFAFSKGDSVVVIGLGRFGGAVAQSLMSLGHDVMGI
                     DKDPEPVQAWADKLTHAVQADSTNETVMLQLGVADFAHAIVGIGSDVSASLMTLMVLT
                     ELGIKDIWLKAMTAEHGKIAQRIGAHHVISPEADMGARVAHLITGRLMDFIEFDDGFA
                     IAKIRAPALTHDQKLADSHVRERFGVTVVGVKRTHEDFQHATPATLIQPGDLLIVSGP
                     TKKIELFAGGGRKP"
     gene            complement(2003..>2516)
                     /locus_tag="VM406_12835"
     CDS             complement(2003..>2516)
                     /locus_tag="VM406_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HVL76893.1"
                     /translation="RTAGFNSIDVGAMTQESWALHYLLMFIGGGSASTAGGVKVGTAA
                     ILALLVISEIRGHPDTEAFGRRVGPAAQRQAITVLVLGSAMVVLGTLLLLRITDFPIS
                     RIIFEVISAFGTVGLSTGITADLPPAGQFILTLLMYFGRVGTITLATSLVLRERRMPY
                     RYPEEHPIVG"
BASE COUNT          470 a          754 c          828 g          464 t
ORIGIN      
        1 atttgaggcc ggcatccgtg atggcggccc gacgaccgtc cggttcaaga atggaaatgc
       61 caagtctttc ctcgagcttt gcaatcgtgt agctgatggc cggctggctg atgtgcagca
      121 cttcggccgc ccgggcgaaa ctgccatggt ggaccactgc aagcaaggtg ttccattgct
      181 tgagactgat gtggctgcgg gtcaggtgct ggcgggcgtg cttggcgacg accggctgct
      241 gcgggaccag cgggtaaggc gcagtaggcc aggggcagga ggtgcgtttc atcggggatc
      301 cttattccat gcgtattcgt tgaacgatga tgcaaggccg cctgcgcgac acgcagacga
      361 cgggatcctc ccttgcttgc cggcgtatca gttactcgac gttgttcaac agcacgcccc
      421 tctcgccggg cgttggtgcc cgtacttgcg caaacaggag gggaggggac gaggagacat
      481 gccgttgcgg gtgagatagg cggcatgagg tgctgagaag gcgtaagtag cgattccagt
      541 aagtcgatat tgtgaagcga acgataccgg aagtcggtct ctcaccacaa tctctttcat
      601 gacaaattgt taatcaagat ttgatctggc tcatagcctc cggtactggc cttgctgaag
      661 gtcattgcct ggcccggatt ttgagttgcg gccactcttt gcactcggat gcagcttacc
      721 ggcccatgcg cactacgctg tcaggcagca aagcctgcaa cttccgggca acggtcatca
      781 gaatattgat ttcgacgaga tgtccgacgc gcttgcgcac taccatgtct ttaaagggga
      841 tgctgatacc cggcagcagg agcgcctgcc gcgcatatcg ggatatggag actttggttg
      901 cgcacggagt cgccgagaca caggcaatgg agtcgatcat gcagcgtgta attgccgccg
      961 cggtggcgct gacgctatcc ggcgccgtcg cgccgcacgg cgcattggcc agccaggagc
     1021 tggcaaggtc caaggggtgt gtcgcgtgcc atgcgatgga caagaaactg gtcggcccgg
     1081 cattcaagga tgtcgccgca cggtacgcgg acgagagggg ggccgaggac cggttggtcg
     1141 agaaggttct caagggtagc cagggcgcat ggggcgcgat tcccatgccg gcgaacacga
     1201 atgtttccca ggaagaggcc agggcactcg tgcggtggat cttgcagggc gggtaggacg
     1261 ctcgtctcgg aatgcaggcg gcccctagag catgcggcgc cggtcgtcgc ggcgctacgg
     1321 ctttctgccg ccacccgcga acagctcaat cttcttggtc ggaccggaca cgatcagcag
     1381 gtcgcccggc tggatgaggg tcgcgggcgt cgcatgctgg aagtcttcat gggtgcgctt
     1441 gacgcccacc accgtcacgc cgaagcgttc gcgcacgtgg gaatccgcga gcttctggtc
     1501 gtgcgtgagc gccggcgcgc gaatcttcgc gatcgcaaag ccgtcgtcga actcgatgaa
     1561 atccatcagc cggccggtga tcaggtgcgc gacccgcgcc cccatgtccg cttcaggcga
     1621 gatcacgtga tgggcgccga tgcgttgggc gatcttgccg tgctcggccg tcatcgcctt
     1681 gagccagatg tccttgatcc cgagttcggt cagcaccatc agcgtcatca ggctggccga
     1741 cacatccgac ccgatgccga cgatcgcgtg cgcgaaatca gccacgccca gttgcagcat
     1801 caccgtctcg ttggtcgaat ccgcctgcac cgcgtgggtc agcttgtcgg cccaggcctg
     1861 caccggctcc gggtccttgt cgatgcccat cacgtcgtgt cccaggctca tcagcgattg
     1921 cgcgacggcg ccgccgaaac gtcccagccc gatgacgaca acgctgtcgc ccttcgagaa
     1981 ggcgaactgc tccgtaaaaa tcctacccaa caattggatg ctcctcagga tagcggtatg
     2041 gcatgcgccg ctccctcaat accagcgagg tcgccagcgt gatggtgccg acccgcccga
     2101 agtacatcag gagggtcagg atgaactggc cggcgggcgg caggtcggcc gtgatgccgg
     2161 tcgacagtcc gacagtgccg aaagccgaaa tgacttcgaa gatgatgcgg ctgatcggga
     2221 aatcggtgat gcgcagcagc agcagcgtac cgagcacgac catcgcgctg cccagcacca
     2281 gcaccgtaat cgcctgacgc tgggcggcgg gtccgacgcg gcggccgaag gcctcggtgt
     2341 cgggatggcc gcggatctcg gaaatcacca gcagcgcgag gatcgccgcg gtgccgactt
     2401 tcacgccgcc ggcggtgctc gcgctgccgc caccgatgaa catcagcaga tagtgcagcg
     2461 cccagctttc ctgcgtcatc gcgccgacgt cgatggaatt gaagccggcg gtgcgc
//
LOCUS       DATLGF010000420         5255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_10231,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000420 DATLGF010000000
VERSION     DATLGF010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10231"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /gene="hpnE"
                     /locus_tag="VM406_12840"
     CDS             <1..1022
                     /gene="hpnE"
                     /locus_tag="VM406_12840"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HVL76894.1"
                     /translation="GTGMDFVAPRLPAPWHVIVALLRARGLTRADKLALARFSSTMRW
                     IDWRMNEDCSVAELLERHDQTAALVQLMWRPLCIAALNTPPERASAQVFLNVLRDSLG
                     ARRSASDMLLPRVDLSTLFPEAAARFVEQHRGRVLRGQAVRTLERTAAGWQVDGEDFD
                     AVVAALPPSTAAGLLEGVCANHLLAGLRHEPITTCYLQYEAALRLEQPFFALVEDPVN
                     GDWGQFVFDRGQLDSGAAGMLAVVVSASGDALAQGHEALAAAIAAQLARVFGKPELER
                     PVWRKVISEKRATFSCTPELARPENDTGCPGLVLAGDYTAGDYPATLEGAVRSGVRAA
                     KLLTG"
     gene            1062..2702
                     /locus_tag="VM406_12845"
     CDS             1062..2702
                     /locus_tag="VM406_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HVL76895.1"
                     /translation="MQHVLEDPFYYLGNFERALDWVGARYCHLLDDAEQAFIARFPTL
                     PRPSRALLVRMVMRKGSHFRSSKLRYDEIGCPRKAAAPLVAEGWVDEQPVLEIDTLFA
                     LMTKAELAAAFSLAPAVAAMPKAELLEHLRSAHEAPRPYAAWHPASTDCVYLNAVDPL
                     CERLRLLFFGNAYQDWTEFVLADLGIYRYEQVDLSDNALGFRTRADVDHFLLLQDCRQ
                     RLAGGEPAAEVLAALPAALSDNPWLAARREKLLFSIGQQLEREDRLEEARALYAGCRH
                     AGARLRFVRVLERLERHDEAWRLASAASAHPENESELQQVARMLPRLARKLGLARPAL
                     AGAPPFERIDLVLPGPDEAGVELAVAQRLREPQAPVFYVENALLSSLFGLLCWRAIFA
                     PLPGAFFHPFHSGPADLHSPDFHARRAALFDACFAELDSDAYRRTILATYEEKSGIAS
                     PFVAWPLLDPGLLELALDCIPASHLRPIFERMLFDIGSNCAGLPDLIQFFQDERRYRM
                     IEVKGPGDRLQDNQIRWLHRFAQHDIPVAVCYVRWEDA"
     gene            2699..4960
                     /locus_tag="VM406_12850"
     CDS             2699..4960
                     /locus_tag="VM406_12850"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HVL76896.1"
                     /translation="MMYRIAVRELAEFTAKAGDLDLRFSPAPTAQEGIAGHQFVAGRR
                     GSGYQTEIQLESECAGLAVRGRADGFDPARNRVEEIKTYRGELERMPANHRQLHWAQA
                     KFYGWMLCEKFGLAEIGVALVYFEIGSQRETVLEEHCSADTLRAFFEAHCARFAAWAE
                     QETAHRQARDDALRALAFPHAQFRAGQRRLAEAVFRAARHRRCLLAQAPTGIGKTIGT
                     LFPMLKAAADSETGLDKVFFLTAKTPGRALALEAVQVLRKDSPLPLRTIELIARDKAC
                     EHPDKACHGESCPLAQGFYDRLPQAREAALREPMLTRTALREAALAHGVCPYYLAQDL
                     VRWADVIVGDYNYYFDASALLYALAAENGWRVGLLVDEAHNLLERARAMYSAELDQGT
                     LRQLRRQAPAGLASSFERLHRGWNALAREDEAAYRICEALPGRLVGTLQQTVAEIGAF
                     YAEHPGHADPLLQRFYFDALHFLRMAEAFGPHSLFDIAVVAGGRARNARLGIRNVIPA
                     PFIGARFRAAQAATLFSATLNPWYFHCDTLGLPDTLAWVEVESPFQASQLAVRIARKV
                     STRYRDRTASLGNVVELMGKAWSERPGNYLAFFSSFEYLRQAADALHARFPDIPVWEQ
                     ARGMDEAAREAFLAQFRPAGAGIGFAVLGGAFAEGVDLPGERLIGAFIATLGMPQVNP
                     FNEAVKARMQAHFGSGHEYAYLYPGLRKVVQAAGRVIRSPDDRGAVYLMDERFARSEV
                     RALLPSWWEPGLD"
     gene            5096..>5255
                     /locus_tag="VM406_12855"
     CDS             5096..>5255
                     /locus_tag="VM406_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVL76897.1"
                     /translation="MANTEIKRIDLALQGGGAHGAFTWGVLDRLLDEERLEIDGVSGT
                     SAGAMNAVV"
BASE COUNT          778 a         1803 c         1835 g          839 t
ORIGIN      
        1 acggcaccgg catggatttc gtcgcgccgc gcctgccggc gccgtggcac gtgatcgtcg
       61 cgctgctgcg ggcgcgcggc ctcacgcgcg cggacaagct ggcgctggcg cgcttttcga
      121 gcacgatgcg ctggatcgac tggcgcatga acgaggattg cagcgtcgcc gaactgctgg
      181 agcgtcacga ccagaccgcg gcgctggtgc agctgatgtg gcggccgctg tgcatcgcgg
      241 cgctgaacac gccgccggaa cgggcctcgg cccaggtgtt cctgaacgtg ctgcgcgaca
      301 gcctcggcgc gcgccgcagc gcatccgaca tgctgctgcc gcgcgtggac ctgagcacgc
      361 tgtttccgga agcggcggcg cgcttcgtcg aacaacatcg cgggcgcgtg ctgcgcgggc
      421 aggcggtgcg aactctggaa cggaccgcgg cgggctggca ggtcgacggc gaggacttcg
      481 atgcggtggt cgccgccctg cccccatcca cggcggccgg tctgctggaa ggcgtgtgcg
      541 cgaaccacct gctcgcgggg ctgcgtcacg aaccgatcac cacctgctac ctgcagtacg
      601 aggcggcgct gcgcctggag cagccgttct ttgcgctggt cgaagatcct gtcaacggcg
      661 actggggcca gttcgtgttc gaccgcggcc agctcgacag cggcgcagcc ggaatgctgg
      721 cggtggtggt cagcgcatcc ggcgatgcgc tggcgcaggg acatgaagcg ctggcagcgg
      781 cgatcgccgc ccagcttgca cgcgtgttcg gcaaaccgga gctggagagg ccggtctggc
      841 gcaaggtgat ttccgagaag cgggcgacat tttcctgcac gccggagctg gcgcggccgg
      901 agaacgacac cgggtgcccg ggactcgtgc tggccggcga ctacaccgcc ggcgactatc
      961 cggccacgct ggaaggcgcc gtgcgcagcg gcgtgcgggc ggcgaagctg ctgacaggct
     1021 gaacacgccg cgcaaccgcg tcctgctggg ataatggcgg gatgcagcac gtcctggaag
     1081 atccgtttta ctaccttggc aacttcgaac gcgcgctcga ctgggtcggg gcgcgctact
     1141 gccacctgct tgacgacgcc gagcaggcat tcatcgcgcg ttttcccacc ctgccccgtc
     1201 cttcccgtgc gctgctggtg cgcatggtga tgcgcaaggg cagccatttc cgttccagca
     1261 agctgcgcta tgacgagatc ggctgcccgc gcaaggcggc cgcgccgctg gtggccgagg
     1321 gctgggtcga cgaacagccg gtgctcgaga tcgacacctt gttcgcgctg atgaccaagg
     1381 ccgagcttgc cgctgccttc agtcttgcgc cggcagtcgc ggccatgccg aaggcggaac
     1441 tgctcgagca tctgcgctcg gcgcacgaag cgccgcgccc ctacgccgcc tggcatccgg
     1501 cgagcacgga ctgcgtctac ctgaatgcgg tggacccgct gtgcgagcgc ttgcgcctgc
     1561 ttttctttgg caatgcctac caggactgga ccgagttcgt gctggcggac ctcggcatct
     1621 accgctacga acaggtcgac ctgagcgaca acgcgctcgg gttccgcaca cgcgcggacg
     1681 tcgatcattt cctgctgctg caggactgcc ggcagcggct ggccggaggc gagccggccg
     1741 ccgaggtgct tgcggcgctg ccggcggcgc tgtccgacaa tccctggctc gccgcgcggc
     1801 gagagaagct gctgttctcg atcggtcagc agctcgaacg cgaggacagg ctggaggagg
     1861 cgcgcgcgct gtatgccggc tgccgccacg ccggcgcacg cctgcgcttt gtgcgcgtgc
     1921 tcgagcgcct cgaacgccac gacgaagcct ggcggctcgc cagcgcggcc agcgcgcacc
     1981 cggagaacga gagcgaactg cagcaggtcg cgcgcatgct gccgcgcctg gcgcgcaagc
     2041 tcggcctcgc caggccggca ctggccgggg cgccgccatt cgaacggatc gacctggtcc
     2101 tgcccggccc ggacgaagcg ggcgtcgaac tggccgtcgc gcagcggctg cgggagccgc
     2161 aggctccggt gttttacgtc gagaatgcgc tcttgagttc gctgttcggt ctgttgtgct
     2221 ggcgcgcgat attcgcaccg ttgccgggcg ccttcttcca tccgttccac agcggaccgg
     2281 cggacctgca cagcccggat ttccacgcgc gccgcgccgc actgttcgac gcctgtttcg
     2341 ccgagctcga cagcgacgcc taccggcgca cgatactggc cacgtatgag gagaaatccg
     2401 gcatcgcctc gcccttcgtc gcctggccgc tgctggaccc gggcctgctc gaactggcgc
     2461 tcgactgcat ccccgcttcc cacctgcgcc ccatcttcga gcgcatgctg ttcgacatcg
     2521 gcagcaactg cgccggcctg cccgatctga tccagttctt ccaagacgag cggcgctacc
     2581 ggatgatcga ggtcaagggg ccgggcgacc ggctgcagga caaccagatc cgctggctgc
     2641 acaggttcgc gcagcatgac atcccggtag ccgtgtgcta tgtgcgctgg gaggatgcct
     2701 gatgtaccgg atcgcggtgc gcgaactggc cgagttcacg gccaaggcgg gcgacctcga
     2761 cctgcgcttt tcgccggcgc ccaccgcgca ggaaggcatc gccggccacc agttcgtcgc
     2821 cggacggcgc ggctccggct accagaccga gatccagctg gaatccgaat gtgcggggct
     2881 ggcggtgcga ggacgcgccg acggtttcga cccggcgcgg aaccgggtcg aggaaatcaa
     2941 gacctatcgc ggcgagctag agcgcatgcc ggcgaatcac cgccagctgc actgggcgca
     3001 ggcgaagttc tacggctgga tgctgtgcga gaagttcggg ctggccgaga tcggcgtggc
     3061 gctggtctac ttcgagatcg gcagccagcg cgaaaccgtg ctcgaggagc actgcagtgc
     3121 cgacacgctg cgcgctttct tcgaagcgca ttgcgcgcgc ttcgcggcct gggccgaaca
     3181 ggaaacggcg caccgccagg cgcgtgacga cgcgctgcgc gccctcgcct tcccgcacgc
     3241 gcagttccgc gccggacagc gccggctcgc cgaggcggta tttcgcgccg cgcgccatcg
     3301 ccgctgcctg ctcgcgcagg cgccgaccgg catcggcaag acgataggca cgctgttccc
     3361 gatgctcaag gccgccgccg attccgaaac gggactcgac aaggtgttct tcctgaccgc
     3421 gaagacgccc gggcgcgcgc tcgcgctcga ggcggtccag gtcctgcgca aggacagtcc
     3481 cctgccgctg cgcacgatcg agctgatcgc gcgtgacaag gcctgcgagc atccggacaa
     3541 ggcctgccac ggcgaatcct gcccccttgc gcagggcttc tacgaccggc tgccgcaggc
     3601 gcgtgaggcg gcgctgcgcg agccgatgct gaccaggacc gcattgcgcg aggccgcgct
     3661 tgcccatggc gtctgcccgt actacctggc ccaggacctg gtgcgttggg cggatgtgat
     3721 cgtgggcgac tacaactact acttcgacgc cagtgcgctg ctgtatgcgc tggcggcgga
     3781 gaacggctgg cgcgtcggcc ttctggtcga cgaggcgcac aacctgctcg aacgcgcgcg
     3841 cgccatgtac tcggcggaac tcgatcaggg cacgctgcgc cagttgcgcc gccaggcgcc
     3901 ggcggggctg gcgtcgagct tcgagcgcct gcatcgcggc tggaatgcgc tggcgcgcga
     3961 agacgaggcc gcgtaccgca tctgcgaagc ccttcccggc cgcctggtcg gcacactgca
     4021 acagacggtt gcggagatcg gcgcgttcta tgccgagcac cccggccatg ccgatcccct
     4081 gctgcagcgg ttctacttcg acgcgctgca tttcctgcgc atggccgagg cgttcggccc
     4141 gcactcgctg ttcgatatcg ccgtcgtcgc cggcggccgc gcgcgcaacg cccggctcgg
     4201 gatccgcaac gtgatcccgg cgcccttcat cggcgcacgc tttcgcgcgg cccaggccgc
     4261 gacgctgttc tccgccacgc tcaacccctg gtacttccac tgcgacacgc tgggcctgcc
     4321 ggacacgctc gcctgggtcg aggtggaatc gccgttccag gccagccagc tcgcggtgcg
     4381 gatcgcacgc aaggtgtcga cccgctaccg cgaccgcaca gcctcgcttg ggaatgtggt
     4441 cgaactgatg ggcaaggcct ggagcgagcg gccgggcaac taccttgcct tcttcagcag
     4501 tttcgagtac ctgcgacagg ccgcggatgc gctgcacgcg cggtttccgg acattcccgt
     4561 ctgggagcag gcgcgcggga tggacgaggc ggcgcgcgag gcgttcctcg cgcagttccg
     4621 gcccgccggc gccggcatcg gcttcgccgt gctcggcggc gcgttcgccg agggcgtcga
     4681 tttgcccggc gagcggctga tcggcgcctt catcgcgacg ctggggatgc ctcaggtcaa
     4741 tccgttcaac gaagcggtga aggcgcgcat gcaggcgcat ttcggcagcg gccacgagta
     4801 cgcctacctc tatcccggct tgcgcaaggt cgtgcaggcg gccgggcggg tgatccgttc
     4861 gccggacgac cggggcgcgg tctacctgat ggacgagcgc tttgcccgca gcgaggtacg
     4921 cgcgctgctg ccgtcctggt gggagccggg gctggactga gacgcgaacg aaaagcaacc
     4981 ggcttttgcg tatcggtatc ggtgcgcttg gcggattttt tccgcccccg cgcactcttt
     5041 ccgagagact ttgtccacgc attggcgtag ctgcattcac gggagaaact gaagcatggc
     5101 aaacaccgag atcaaacgca tcgacctcgc gctgcagggc ggcggcgcgc atggcgcctt
     5161 cacctggggc gtgctggacc ggctgctcga cgaggagcgt ctggagatcg acggcgtgag
     5221 cggaaccagc gccggcgcga tgaacgccgt agtcc
//
LOCUS       DATLGF010000421         2717 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_153097, whole genome shotgun sequence.
ACCESSION   DATLGF010000421 DATLGF010000000
VERSION     DATLGF010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153097"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VM406_12860"
     CDS             <1..260
                     /locus_tag="VM406_12860"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142027.1"
                     /note="threonine deaminase; threonine dehydratase; in
                     Escherichia coli, IlvA is part of the isoleucine
                     biosynthetic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HVL76898.1"
                     /translation="LAQNELLYRFEFPERPGALMRFLDSMSPNWNISLFHYRNQGGDV
                     GRILVGLQVPKKEMRDFRAFLSTLGYRHWDETDNPAYKLFL"
     gene            303..1142
                     /gene="queF"
                     /locus_tag="VM406_12865"
     CDS             303..1142
                     /gene="queF"
                     /locus_tag="VM406_12865"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142029.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HVL76899.1"
                     /translation="MTVSHAPEGSPLGKPVAYQGQYDPSLLFPIPRGPKRDELGIQGT
                     LPFFGVDIWNAYEVSWLNPRGKPQVAIATFTVPADSPNIVESKSFKLYLNSYNQTRLE
                     SPEALVARLQADLSAGFGANVHVKLTLPEAFGAIRMSELEGLLLDRLDIEVSEYRPNP
                     ALLRAAHEEAIVEETLVSHLLKSNCLVTGQPDWGSVQIRYVGPQIDQEGLLQYLIGFR
                     DHDEFHEQCVERIFMDIMRACRPQKLAVYARYTRRGGLDINPWRSNFTTGSRPPNLRG
                     ARQ"
     gene            1351..1818
                     /locus_tag="VM406_12870"
     CDS             1351..1818
                     /locus_tag="VM406_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HVL76900.1"
                     /translation="MTNLAKILPVSNVALDLEVSSKKRAFEQAGLLFENNCSIARSTV
                     SDNLFARERLGSTGLGHGVAVPHGRIKGLKSPLAAFVRLKEAIPFESPDGQPVRLLVF
                     LLIPDHVTQQHLEILAEVAEMFSDEALRAELASDPDPASVHKRIVAWQPSVHA"
     gene            1829..>2717
                     /gene="hprK"
                     /locus_tag="VM406_12875"
     CDS             1829..>2717
                     /gene="hprK"
                     /locus_tag="VM406_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HVL76901.1"
                     /translation="MPTTTHLTIQQLYDDTRDSLQLGWFAGFAGGERRISGDATSAAD
                     QVGHLNLIHPGRIQVFGHQEVRYYNKLSPNSRKYQTDELVAGEPPAFIIAQGLETPPD
                     ILAVCDEKDIPLFSTPLPAAQVIDYLRVYLSKELAQRITMHGVFMDVLGVGVLITGES
                     GLGKSELGLELISRNHGLVADDAVEFSRIAPNMIEGRCPPLLQNLLEVRGLGLLDIKT
                     IFGETAVRRKMRLKLIVHLVRRKTLEENYERLPLHSQTQDVLGLPIRKVVIPVEAGRN
                     LAVLLEAAVRNTILQLRGID"
BASE COUNT          510 a          926 c          797 g          484 t
ORIGIN      
        1 cgctggcaca gaacgagctt ctttatcggt tcgagtttcc ggaacgcccg ggcgcgctga
       61 tgcgattcct ggacagcatg tccccgaact ggaacatcag cctgttccat taccgtaacc
      121 agggcggcga cgtcggccgc atcctggtcg gcctgcaggt tccgaagaag gaaatgaggg
      181 acttccgcgc cttcctgtcc acgctcggct accgccactg ggacgaaacg gacaaccctg
      241 cgtataaact gtttttgtaa tccgacggcg gcgccgcggc gccgccacaa gccgcaccca
      301 tcatgaccgt ctcccatgcc cctgaaggct cgccgctcgg caagccggtc gcctaccagg
      361 gccaatacga cccctcgctg ctattcccga tcccgcgcgg ccccaagcgc gacgaactcg
      421 gcatccaggg cacgctgccg ttcttcggcg tcgatatctg gaacgcctac gaagtgtcat
      481 ggctgaaccc gcgcggcaag ccgcaggttg ccatcgccac cttcaccgtg ccggccgact
      541 cgcccaacat cgtcgagtcc aagtcgttca agctgtatct gaattcctac aaccagacgc
      601 ggctcgaatc gccggaggcg ctggtcgcgc ggcttcaggc cgacctttcg gccggcttcg
      661 gcgccaacgt ccatgtcaag ctgacactgc ccgaggcctt cggcgcgatc cggatgtcgg
      721 aactggaagg cctgctgctg gaccggctgg acatcgaagt cagcgaatac cgcccgaacc
      781 cggcattgct gcgcgccgcg cacgaggaag cgatcgtgga agagacgctg gtctcgcacc
      841 tgctcaagtc caactgcctg gtgaccggcc agccggactg gggcagcgtg cagatccgct
      901 acgtcggtcc gcagatcgac caggaagggc tgctgcagta cctgatcggg tttcgcgacc
      961 atgacgaatt ccatgaacag tgcgtcgaac gcattttcat ggacatcatg cgcgcctgcc
     1021 gaccacagaa gcttgcggtg tatgcccgct atacaaggcg gggcggcctt gacatcaatc
     1081 cctggcgcag caacttcacg accggctccc gcccgccgaa tctgcgcggt gcgcggcaat
     1141 gaccttgtcc acgggaaact ttccggcgat gtacgagtct gacacgtcca ggaccgtccc
     1201 tgctgccacg gctcgaaccc aggcgcgatt cctgccccgc gtcaagagtc gcaccgatcg
     1261 ggcgttctat tctgcgacag tacgaagctt caaacaggcc tataatgcgt ctcgaacgct
     1321 ggcattgtgc aatgcatcat cagtggaatc atgaccaatc tcgccaaaat cctgcccgtc
     1381 tccaatgtcg cgctcgacct ggaggtctcc agcaagaagc gcgcgtttga gcaggccggt
     1441 ctgctattcg agaacaattg cagcattgcc cgctcgaccg tttccgacaa cctgttcgcg
     1501 cgcgagcggc tcggttccac cggcctcggg catggcgtgg ctgtccctca cgggcgcatc
     1561 aagggtttga agtccccgct ggcggcattt gtacggctca aggaagcaat cccgttcgaa
     1621 tcgccggacg ggcagccggt gcggctcctg gtcttcctgc tgattcccga tcacgtgacc
     1681 cagcaacacc tcgagatact cgccgaggtt gccgaaatgt tttccgacga agcgctgcgc
     1741 gcggaactgg cctccgatcc ggatccggct tcggtccaca agcgcatcgt ggcctggcag
     1801 ccgagcgtgc acgcctaggc ataccgccat gcctaccacc acccatctca cgatccagca
     1861 gctctacgac gatacgcgcg attcgctgca gctgggatgg tttgccggct ttgccggcgg
     1921 cgaacgccgc atttccggcg acgccacttc cgccgccgac caggtcgggc acctgaatct
     1981 gatccacccc ggacgcatcc aggtattcgg ccaccaggag gtgcgctact acaacaagct
     2041 ctcgccgaac tcccgcaagt accagaccga tgaactggtc gccggcgaac cgcccgcctt
     2101 catcatcgcc cagggactgg aaacgccgcc tgacattctc gcggtctgcg acgaaaagga
     2161 catcccgctc ttttcgacgc cgctgcccgc ggcccaggtg atcgactacc tgcgcgtgta
     2221 tctgtccaag gagctggccc agcgcatcac gatgcacggc gtgttcatgg acgtgctcgg
     2281 cgtgggcgtg ctgattaccg gcgaatcggg gctgggcaag agcgagctcg gcctggagct
     2341 gatctcgcgc aaccacgggc tggtggcgga cgacgcggtc gaattctcgc gcatcgcccc
     2401 gaacatgatc gaaggccgct gcccgccgct gctgcagaac ctgctcgaag tgcggggact
     2461 tggcctgctc gatatcaaga ccatctttgg cgaaaccgcg gtgcgccgga agatgcgcct
     2521 gaagctgatc gtacacctgg ttcggcgcaa gacgctggaa gagaactacg aacgcctgcc
     2581 gctgcattcc cagacccagg acgtgctcgg cctgccgatc cgcaaggtgg tgatcccggt
     2641 cgaggccgga cgcaacctcg ccgtgctgct cgaggccgca gtgcgcaaca ccatcctcca
     2701 gttgcgcggc atcgaca
//
LOCUS       DATLGF010000422         4674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_172996, whole genome shotgun sequence.
ACCESSION   DATLGF010000422 DATLGF010000000
VERSION     DATLGF010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172996"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1390
                     /locus_tag="VM406_12880"
     CDS             221..1390
                     /locus_tag="VM406_12880"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HVL76902.1"
                     /translation="MLNTALKRLKLKGRSLLPIVQGGMGVGISAHRLAGNVARLGGFG
                     TISSVDLRRHHPDLMAETGKSRDKAMIDRANLVALDREIRAAKQLADGNGVVGVNVMR
                     AVAEYASYVRQTCESGADAVVVGAGLPLDLPELTAEHPDVALLPILSDVRGIALILKK
                     WMRKNRLPDAIVIENPRYAAGHLGAAKIEDVDQPHFAFPEVIEGTQALFRELGIESER
                     IPLVAAGGVNSPEQVKRLLDMGASAVQLGTAFAVTEEGDAHINFKTVLAEAKPEDIVT
                     FMSVAGLPARGVKTPWLANYLDKEAKLQRKAGPKPCTVGFDCLAQCGLRDGIAKAGQF
                     CIDTQLAFALNGDVKRGLFFRGSEKLPFGSEIRSVRELIEYLLNGVLPAPAGVAA"
     gene            complement(1588..2025)
                     /locus_tag="VM406_12885"
     CDS             complement(1588..2025)
                     /locus_tag="VM406_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVL76903.1"
                     /translation="MFNKILVPTDGSPLSEKAVSTAVEWARSNGAALVVLSVAEPHYA
                     PQLAEGSTVVDANAYNRKMLEIAQANVAAAAAAAREAGVPCETLVRTSMNPHEEIVNA
                     SKERACDVIVMASHGRKGLRALFVGSETHKVLAYSTVPVLVVR"
     gene            complement(2065..2916)
                     /locus_tag="VM406_12890"
     CDS             complement(2065..2916)
                     /locus_tag="VM406_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVL76904.1"
                     /translation="MSYKTILVHVDKDQHAAERIALASRIANAQNAHLVGVAPTGVSR
                     LVYQTRYVYDGGRMASNLDEHLDRLRAEAASALNAFEAEVDKIGARSFESRPVDDEAG
                     AAISLQARYADLVVLGQYDPEHASAVTMSDFPEFVVMNSGRPVLLVPYTGHFPEAGKR
                     VMIAWDASTSATRAVTAAIPLLKQAAQVDVVVFNGESSDEGHGEQPGADIALYLARHG
                     VQVNVVRQKTSGIDVGNALLSMASDLGSDMMVMGGYGHSRFREILLGGVTRTVLQSMT
                     VPVLMAH"
     gene            3327..4674
                     /gene="hemN"
                     /locus_tag="VM406_12895"
                     /pseudo
     CDS             3327..4674
                     /gene="hemN"
                     /locus_tag="VM406_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924200.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
BASE COUNT          884 a         1447 c         1513 g          830 t
ORIGIN      
        1 ccaagtcgta ctcctttcgt atgcaaactt gccattctaa tcaggatgat gattggaaaa
       61 aatgcatgtt tgtcgtggtt ggactctaac accccgaccg ttgacagggc cggtcgcacg
      121 tgtccaacag gcgggcacgg aggcgcttga ctcagatcaa acatgtaaaa aatcccgctt
      181 gatagattcg caggtcgctt tttcgaatgg aaagaccgct gtgctgaata ctgctctcaa
      241 acggttgaag ctcaagggca ggagcctgct gcccatcgtg cagggaggga tgggcgtcgg
      301 catctccgcg caccgtctgg ccggaaacgt cgcgcgtctg ggcggcttcg gcacgatttc
      361 cagcgtggac ctgcgccgtc atcatccaga cctgatggcc gagaccggca agtcgcgcga
      421 caaggcgatg atcgaccggg cgaacctggt tgctctcgat cgcgagatcc gggcagcgaa
      481 acagctggcc gacggcaatg gcgtggtcgg cgtgaacgtg atgcgcgcgg tggccgagta
      541 cgcgtcctat gtgcggcaga cctgcgagag cggggcggac gcggtcgtgg tcggcgcggg
      601 cctgccgctg gacctgcccg agctgaccgc cgagcatcca gacgtggcgc tgctgcccat
      661 cctgtcggac gtgcgcggga tcgcgctgat cctgaagaaa tggatgcgca agaaccggct
      721 gccggatgcg atcgtgatcg agaatccgcg ctacgcggcc ggccatctcg gcgcggcgaa
      781 gatcgaggat gtcgaccagc cgcatttcgc gtttcccgaa gtgatcgaag gcacgcaggc
      841 gctgttccgg gaactgggca tcgagagcga acgtatcccg ctggtggccg ccggcggggt
      901 gaacagcccg gagcaggtga agcgcctgct ggacatgggg gcgagcgcgg tgcagctcgg
      961 caccgcattc gcagtcaccg aggagggcga tgcccacatc aacttcaaga cggtgctggc
     1021 ggaggcgaag ccggaagaca tcgtcacctt catgagcgtg gccgggctgc ccgcgcgcgg
     1081 cgtgaagacg ccgtggctcg cgaactacct ggacaaggaa gccaagctgc agcgcaaggc
     1141 cggcccgaag ccgtgcacgg tgggcttcga ctgcctcgcg caatgcgggc tgcgcgacgg
     1201 catcgcaaaa gccggacagt tctgtatcga tacccagctc gcattcgcgc tcaacggcga
     1261 cgtcaagcgc ggactgttct tccgaggctc ggagaagctg ccgttcggca gcgagatccg
     1321 cagcgtgcgc gaactgatcg aatacctgct caatggtgtg ctgccggcgc cggccggagt
     1381 ggcggcatga acagggcact gccctgttcc gccgacggcc tgcccgcgct cacgccaagc
     1441 gcgatccagt ccgccggctg cagaggggga ttcaggcgcc gcgcgtcgta gcgtgcgcct
     1501 gaagcgccgg gattctcacc gcgcgcaacg cgaggttgcc gtccgccttc gtagaagtgc
     1561 ggaggtccca ggttgccacc acggcgatca ccggactacc agtaccggca cggtcgaata
     1621 cgcgagaacc ttgtgggttt cgctgccgac aaacagggcg cgcaggccct tgcgtccatg
     1681 cgaggccatc acgatgacat cgcaggcgcg ttccttcgat gcattcacga tctcttcatg
     1741 cggattcatc gaggtgcgca ccagcgtctc acacggaacg ccggcttccc tggccgccgc
     1801 ggcggccgcg gctacgttcg cttgcgcgat ttcgagcatc ttgcggttgt aggcgtttgc
     1861 gtcaacgacg gtcgagcctt ccgcaagctg cggcgcgtaa tgcggttcgg cgaccgagag
     1921 cacgacgagc gcggcgccgt tcgaccgcgc ccactcgacc gcggtggaaa cggccttttc
     1981 agacaacggt gagccgtcgg tggggacaag aatcttgttg aacatggttg tagtcaaaga
     2041 gtgaatgtga aggcggacgt ccgctcagtg agccatcagc accggcacgg tcatcgattg
     2101 gagcacggtg cgcgtgacgc cgccgagcag gatctcgcgg aagcgcgagt ggccataacc
     2161 gcccatcacc atcatgtccg agccgaggtc gctggccatc gacagcagcg cgttgcctac
     2221 gtcgatgccc gacgtcttct ggcgcaccac gttgacctgc acgccatggc gcgcgaggta
     2281 gagcgcaata tcggcgccgg gctgctcgcc atgaccctcg tcgctcgact cgccattgaa
     2341 gaccacgaca tcaacctgcg cagcctgctt gagcagggga atggccgcgg tgaccgcgcg
     2401 cgtggcgctg gtgctcgcgt cccacgcgat catcacgcgc ttgccggctt ccgggaaatg
     2461 accggtgtaa ggaacgagca acaccggccg tcctgaattc atgacgacga attcgggaaa
     2521 gtccgacatc gtgaccgccg acgcgtgctc cgggtcgtac tggccgagaa cgacgaggtc
     2581 ggcataacgc gcctgcagac tgattgcggc accagcttcg tcatcaaccg gtcgcgattc
     2641 aaacgagcgt gcgccgatct tgtccacttc ggcctcgaac gcattcagtg ccgaggcagc
     2701 ttccgcgcgc aggcgatcca ggtgctcgtc caggttgctc gccatccgcc cgccgtcgta
     2761 cacgtagcgg gtctgataca ccaggcgcga gacgccggtc ggcgcaacgc ccaccagatg
     2821 cgcattctgc gcattggcaa tgcgggaggc gagcgcgatg cgctctgccg cgtgttggtc
     2881 cttgtcgaca tggacgagta tggtcttgta agacatccga cacctcttgt tcgtgatttg
     2941 aaattgttgg ggctgcggat gttcggggac tgcagccgtg aaaacagcgt agggcttgca
     3001 tgaaaatgcg tctacaagaa cgcgattgcg caagcttctg ttttgggcgt ttttgactca
     3061 agtcaaattt ttcgggagat cgggttccga aaggcggcat ggcctgaacc ggactccatt
     3121 cgcgtacagg tactgaaacc ctggcaatgt cgttcgcgca ggagcgacgg cgcaggagtc
     3181 gggtaccccc gggtacaagc cgagatccgc gcagactcgg gcattccggg cgttttgacc
     3241 tggatcaagg tggtgccgac acgcgaatcc ctacactccc gctgtcatga agccccgcga
     3301 tccgcgcggc ggcagcagga gttcaaatgg cattcgccac agccgcagca tccatccttc
     3361 cgccggtcca gttcgacgcg gcgctgatcc gcaagctgag ccgtcaaggg ccgcgctaca
     3421 cctcgtaccc aactgcggac cgcttcaacg agtgcttcgg catgggcgac tacctgcagg
     3481 cggtgaacaa tgtgcgtgcg atgggcgcgc gcaatccgct ctcgctctat atccatgtgc
     3541 cgttctgcga cacggtctgc tactactgcg cgtgcaacaa gatcgtcacg aaaaaccatg
     3601 cgaaggccga gatctacctg acctacctga agcgcgagat cgagatgcag ggcaagctgt
     3661 tcgagggcat gaaccagcag gtcgagcagc tgcatctggg cggcggcacg ccgacttact
     3721 tcagcgacgc gcagatgtcg gatttgctcg cccacgcgcg ccgctggttt cgcttcgcga
     3781 ccgacgaggc gggcgagttc tcgatcgaga tcgacccgcg caccgtcacg ccgcagcgca
     3841 tccacacatt gagagcgcag ggcttcaacc gtttgagcct cggggtgcag gatttcgatc
     3901 cggacgtgca gcgggcggtc aaccggatcc agcccgagca atgcacgctc gacgcgattg
     3961 ctgccgcgcg cgaggccgga tttcgctcgg tgtcgatcga cctgatttac ggcctgccgc
     4021 tgcagaacgt gatcacgatg ggcaagacgc tggcgaaggt gattgccgcc gacccggatc
     4081 gcatctcgat ttacaactac gcgcacatgc cggccttgtt caagccgcaa cgccggatca
     4141 acgaagagga tctgccgagc gcggagacca agctggacat gctgtcactg tgcatccggc
     4201 gcctgaccga cgccggctat gtgtacatcg gcatggatca tttcgccaag ccggacgacg
     4261 agctcgcgat cgcccagcgc cagggccggc tgcaccgcaa ctttcaggga tattcgacgc
     4321 atgccgacgc cgagatggtg tcctgcggcg tctccgcgat cagcgcggtc ggctccacct
     4381 acagccagaa cgaaaagacg ctggaggcgt actacgagcg catcgagcgc ggcgagctgc
     4441 cgatcgcgcg cgggatcaag ctgtcggtcg acgacctgtt gcgccgcctg atcatccaga
     4501 tgttgatgtg caatttcgag ctgtcgatct cgtcgctgga actggcccat ccgatcacgt
     4561 tctacgagta cttcgccaat gagctggggc agttgcgcgt gctctaggct gacggccttg
     4621 tggtcgttga accggagtgg atcacggtga ccccgaaggg gcgtctgctg atcc
//
LOCUS       DATLGF010000423         5308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_10396,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000423 DATLGF010000000
VERSION     DATLGF010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10396"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VM406_12900"
     CDS             <1..415
                     /locus_tag="VM406_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HVL76905.1"
                     /translation="NAMSKSGSVEAAVPALFGVPASTLARASVSASSSNSFQDKGTAS
                     SSNTFSGTITTTVVDVLPNGNLVVSGEKQVALDKGVEYVRFSGVVNPDTIMAGNVVPS
                     TQVADARVEYRTNTRIDKAEMMSQLSRFFLSLLPL"
     gene            426..1556
                     /locus_tag="VM406_12905"
     CDS             426..1556
                     /locus_tag="VM406_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HVL76906.1"
                     /translation="MTTPSSFGVRRLLASALGALCLALGTGAFAQERLLDISSIQGVR
                     QNQLIGYGLVVGLDGSGDQTTQAPFTVQSVVSMLQSMGVTVPPGTSLQLKNVAAVMVT
                     ASLPAFAQPGQTLDVTVSSMGNAKSLRGGTLLMTPLKGADGQIYGIAQGNVLVGGAGA
                     AASGSKAQVNHLSVGRISAGATVERAVPTTLGQADAVFLELHDTSFSTASRVVEAINQ
                     RFGPDTAAAQDGRVIRVRAPQSSDERVAFLGALESLRVTPAQTAAKVILNARTGSVVM
                     NQAVTLDSCAVSHGNLSVVISTEPIVSQPGPFSGGQTVVTTASQIELRSDPGQVMLLK
                     GGTSLADVVKALNAIGATPQDLLAILQAMKAAGSLRAELEII"
     gene            1556..2476
                     /gene="flgJ"
                     /locus_tag="VM406_12910"
     CDS             1556..2476
                     /gene="flgJ"
                     /locus_tag="VM406_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HVL76907.1"
                     /translation="MLDPLDNSHRFALDTRDLGQLRHAAREDSPEALKAVAKQFEAMF
                     MNMLLKSMREATPGDGIFDNEQTRMYTTMLDQQLSQTMASRGIGLADVLVRQLSGSMG
                     SDPMATGAPRAGVPFVAPMPAPAPAPAPASASAAAAWVAAMSEQARPNTAQPEHVRAF
                     QQRLAPHAEEASRMTGIPARFMLGQAALESGWGKREIVGADGTRSHNLFGIKATGGWK
                     GRVVETTTTEYVNGVPEKRVEKFRAYDSYAEAFRDYARLLRSNPRYENVIANARDVHG
                     FAQGLQKAGYATDPNYAAKLTRIITQSLSS"
     gene            2556..4514
                     /gene="flgK"
                     /locus_tag="VM406_12915"
     CDS             2556..4514
                     /gene="flgK"
                     /locus_tag="VM406_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HVL76908.1"
                     /translation="MSSNILGIGQSALAAAQAGLATTGHNIANASTPGYSRQMVVQGS
                     AGAQNAGVGYIGKGTQIVDVRRIYNDLLAAQVRSAQSSQSYVTTYHAQLSRINNQFAD
                     PVAGLSPTLQNFFKGVQDLASNPNLAASRQTALSSAETLAARFQSLAGQMKEIEQGIN
                     GQIQASVGNINVYANQIARLNDAIEKAHATGDGRAANDLLDQRDYALDQLSKETKTTI
                     VKQGESYNVFIGNGQPVVMGARAYELGVVTSPTDPTRLSVGYNNNGQLVPLAESSLSG
                     GTLGGLLEFRSNSLDVAQNQLGRIAIGLAMSFNAQHALGQDLNGDIGGNFFTFGAPSV
                     QPNRSNASSPPAEIGAAITDANALTGSDYKLEAVPGGSYRVTRLSDGNVTSFSSFPQT
                     IDGVRFTLDSGIPLAGDNYLIRPTVNGASGFGVAIADIAKIAAASPIRTAALESNAGS
                     GTISAGEVHATGELASIGLPLTLTHSGNPAAMTGFPPNMEVTVVRPNGSSATVTTDAD
                     GSIAYSPGDTFRFGGISFTIAGNPADGDRFTVGPNTGGVGDSRNAVLLGALQTKNTMN
                     NGTSTYQGAYAQLVSAVGNKTYELELTGRAETRFLEQALTAQQAESGVNLDEEATNLL
                     RYQQAYQAAGKMMQTANQLFELLLRLGG"
     gene            4517..>5308
                     /gene="flgL"
                     /locus_tag="VM406_12920"
     CDS             4517..>5308
                     /gene="flgL"
                     /locus_tag="VM406_12920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HVL76909.1"
                     /translation="MRVSTNTLFEIGAARIGSLQASLNRTSEQIASGRRILSPSDDPV
                     AASQVLELTQTRELNTQFGVNRQGARHSLSMEEQALKSLTSLIQDVQTLLVSAGNGAY
                     DDQQRKFMATELRGRLEDLMGIANTRDGAGNFLFAGYQTTTQPFARSDGSVQYAGDQG
                     VRRLQVGPLRHIAVNDAGGAVFQNVKNGNGLFNVAVPAANTGSGVVSMGNSASGSPLT
                     ARYEVVFVSETEYTIVGPDTDPDDPPVLHTFVPGEMITAGSAQFSI"
BASE COUNT          982 a         1804 c         1719 g          803 t
ORIGIN      
        1 gaacgccatg tcgaaatccg gcagcgtcga agccgcggtg cccgcgctgt tcggcgtgcc
       61 ggcaagcacg ctggcgcgcg cctcggtatc ggccagctcc agcaacagct tccaggacaa
      121 gggcacggcc agttcgagca acaccttcag cggcacgatc acgaccaccg tcgtcgatgt
      181 actgcccaat ggcaacctgg tggtctccgg cgagaagcag gtggcgctgg acaagggcgt
      241 cgaatacgtg cgcttctccg gcgtcgtgaa tcccgacacg atcatggccg gcaacgtcgt
      301 gccatcgacc caggtggccg acgcgcgcgt cgagtaccgc accaataccc gcatcgacaa
      361 ggccgagatg atgtcgcagc tttcgcgctt cttcctgagc ctcttgccgc tgtaaaggag
      421 aatcaatgac cacgccgtcc tcattcggag tgcgacgtct gctggcctcg gcgctcggcg
      481 cgctgtgcct cgcgctgggc accggcgcgt ttgcgcagga acgcctgctc gacatttcgt
      541 cgatccaggg cgtgcggcag aaccagctga tcggctacgg tctggtggtc ggcctggacg
      601 gcagcggcga ccagaccacc caggcgccgt tcaccgtgca aagcgtggtc agcatgctgc
      661 agagcatggg cgtgaccgtc ccgcccggga ccagcctgca gctgaagaac gtagcggcgg
      721 tgatggtgac ggcatcgctg ccggccttcg cacagcccgg ccagacgctg gacgtgacgg
      781 tctcctcgat gggtaacgcc aagagcctgc gcggcggtac gctgttgatg acgccgctca
      841 agggcgccga cggccagatc tacggcatcg cccagggcaa tgtgctggtg ggcggcgccg
      901 gtgccgcggc cagcggcagc aaggcccagg tcaaccacct gagcgtgggc cgcatctcgg
      961 ccggcgccac cgtcgagcgc gcggtgccga ccacgctcgg ccaggccgac gcggtcttcc
     1021 tcgagctgca cgacacctcg ttctccactg cgagccgcgt ggttgaggcg atcaaccagc
     1081 gcttcggtcc cgacaccgcc gccgcccagg acgggcgcgt gatccgcgta cgcgccccgc
     1141 aatcgagcga cgaacgcgtg gccttcctcg gcgcgctcga aagcctgcgc gtgaccccgg
     1201 cgcagacggc ggcgaaggtg atcctcaatg cgcgcaccgg ctcggtcgtg atgaaccagg
     1261 cggtcacgct ggacagttgc gcggtctcgc acggcaatct gtcggtcgtg atcagcaccg
     1321 agcctatcgt cagccagccg gggccgttct cgggcgggca gaccgtggtc accaccgcat
     1381 cgcagatcga actgcgcagc gaccccggcc aggtgatgct gctcaagggt ggcacttcgc
     1441 tggccgacgt ggtcaaggcc ctgaatgcga tcggcgccac gccgcaggac ctgctcgcga
     1501 tcctgcaggc gatgaaggcc gcaggttcgc tgcgcgccga gcttgagatc atctgatgct
     1561 cgacccgctc gacaactcgc accgcttcgc gctcgatacg cgcgacctcg ggcagctgcg
     1621 ccacgccgcg cgcgaggatt cgccggaggc gttgaaggcc gtcgcaaagc agttcgaggc
     1681 gatgttcatg aacatgctgc tcaagagcat gcgcgaagcc acgcccggcg acggcatctt
     1741 cgacaacgag cagacccgga tgtacacgac gatgctggac cagcagctga gccagaccat
     1801 ggcctcgcgc ggcatcggcc tggccgatgt gctggtgcgc caattgtccg gctcgatggg
     1861 tagcgacccg atggcgaccg gcgcgccgcg cgccggcgtc ccgttcgttg caccaatgcc
     1921 ggcaccggca ccggctcccg cgccggcctc cgcttcggcc gcggccgcct gggtggcggc
     1981 gatgtccgag caggcgcggc cgaacacggc gcagccggaa cacgtgcgcg cgttccagca
     2041 gcggctggcg ccgcatgcgg aagaagccag ccggatgacc gggattcccg cgcgcttcat
     2101 gctcggccag gccgcgctgg aaagcggctg gggtaaacgg gaaatcgtcg gcgccgacgg
     2161 cacgcgcagc cacaacctgt tcggcatcaa ggccaccggc ggctggaagg gcagggtggt
     2221 cgagacgacc acgaccgaat acgtgaacgg cgtgccggag aaacgggtgg aaaaattccg
     2281 cgcctacgat tcgtatgcgg aggcgtttcg cgattacgcc cgactgttgc gcagcaatcc
     2341 gcgttatgaa aacgtgatcg ccaatgcgcg cgatgtccac ggttttgcgc agggcctgca
     2401 gaaggccggc tatgcgaccg accccaacta tgccgccaaa ctcacgcgca tcatcacgca
     2461 gtcattgtcg agctgacaag cgctgaaggc cgttctcctc aagtttcccg cgatcgtgcc
     2521 gtagtacacg ttatcgctac aaagcaaacg acgccatgag ttcaaacatt ctcgggatcg
     2581 gccaaagcgc gctggccgcg gcgcaggcag ggttggccac caccggccac aacatagcca
     2641 acgcatctac gcccggctat tcgcgccaga tggtggtcca gggttcggcc ggcgcgcaga
     2701 acgcgggtgt cggctatatc ggcaagggca cgcagatcgt cgacgtgcgg cgcatctaca
     2761 acgacctgct ggccgcgcag gtgcgcagcg cgcagagctc gcaaagctat gtgacgacct
     2821 accacgccca gctgagccgc atcaacaacc agttcgccga tccggtcgcc ggcctgtcgc
     2881 cgacgctgca gaatttcttc aagggcgtgc aggacctggc ctcgaatccg aacctggccg
     2941 cctcgcgcca gaccgcgctg tcgtcggccg agacgctggc cgcgcgcttc cagtcgctgg
     3001 ccggccagat gaaggagatc gagcagggca tcaatggtca gatccaggcc agcgtaggca
     3061 acatcaatgt ctacgccaac cagatcgcgc gcctgaacga tgcaatcgaa aaggcccacg
     3121 cgaccggcga cggccgcgcg gccaacgacc tgcttgacca gcgcgattac gcgctcgacc
     3181 agctgagcaa ggaaaccaag accaccatcg tcaagcaggg cgagagctac aacgtcttca
     3241 tcggcaacgg ccagccggtc gtcatgggcg cgcgcgcgta tgaactcggt gtcgtcacgt
     3301 cgccgaccga tccgacgcgg ctgagcgtcg gctacaacaa caacgggcag ctggtgccgc
     3361 tggccgagag cagcttgtcg ggcggcacgc ttggcggcct gctcgaattc cgcagcaact
     3421 cgctggacgt ggcccagaat cagctcggcc gcatcgcgat cgggctggcg atgagcttca
     3481 atgcccagca tgcgctcggg caggacctga atggcgacat cgggggtaat ttcttcacct
     3541 tcggcgcgcc gagcgtgcaa ccgaaccgca gcaacgcctc gtcgcccccg gccgagatcg
     3601 gcgcggcgat caccgacgca aacgcgctga ccggtagcga ttacaagctg gaagccgttc
     3661 cgggcggttc gtatcgtgtc acccgtctgt ccgacggcaa tgtgacctcg ttctccagct
     3721 tcccacagac gatcgacggc gtgcggttca cgctggacag cggcataccg cttgccggcg
     3781 acaattacct gatccggccg accgtcaacg gcgcgtcggg cttcggcgtg gcgattgccg
     3841 acatcgccaa gatcgccgcg gcttcgccca tccgtaccgc ggcattggaa tcgaacgcgg
     3901 gttccgggac gatcagcgcc ggtgaggtcc atgccaccgg cgagctcgcc agcatcggac
     3961 tgccgctcac gctcacgcat tcgggcaatc ccgccgcaat gacgggattt ccgccgaata
     4021 tggaggtcac ggtggtacgg cccaacggca gcagcgcgac cgtcaccacg gacgccgacg
     4081 gcagcattgc ctactcgccc ggcgatacct tccgtttcgg cggcatcagc ttcacgatcg
     4141 ccggcaaccc ggccgacggc gatcgtttca ccgtgggccc gaataccggc ggcgtcggcg
     4201 acagccgaaa tgcggtgctg ttgggcgcgc tgcagacgaa gaacacgatg aacaacggca
     4261 cctcgaccta tcagggcgct tatgcgcaac tggtcagcgc cgtgggcaac aagacctatg
     4321 aactggaact gaccggccgc gccgaaacgc gcttcctcga acaggcactg accgcccagc
     4381 aggccgagtc gggcgtcaac ctcgacgagg aagcgaccaa tctgctgcgc tatcagcagg
     4441 cctaccaggc ggccggaaaa atgatgcaaa cggcgaacca gctgttcgag ctgctgcttc
     4501 gtcttggagg ttgatcatgc gcgtaagcac caacaccctg ttcgagattg gcgcagcgcg
     4561 catcggctcg ctgcaggcca gcctgaatcg cacgtccgag cagatcgcgt cgggccggcg
     4621 cattctgtca ccgtccgacg atccggtcgc cgcatcgcag gtgctcgagc tgacgcagac
     4681 gcgcgaactc aacacccagt tcggcgtgaa ccggcagggc gcgcgccatt cgctgtcgat
     4741 ggaagagcag gcgctcaagt cgctcacttc actgatccag gacgtgcaga ccctgctggt
     4801 cagcgccggc aacggcgcgt acgacgatca gcagcgcaag ttcatggcga ccgagctgcg
     4861 cgggcggctg gaagacctga tggggatcgc caatacgcgc gacggcgccg gcaacttcct
     4921 gttcgccggg taccagacga ccacccagcc attcgcgcgc agcgacggat cggtgcagta
     4981 tgcgggcgac cagggcgtgc gccggctgca ggtcggcccc ttgcgccaca ttgccgtcaa
     5041 tgatgccggc ggtgcggtgt tccagaacgt caagaacggc aacggcctct tcaacgtcgc
     5101 ggtgccggcc gccaataccg gctccggcgt ggtctcgatg ggcaacagcg ccagcggctc
     5161 accgctgacc gcgcgctacg aggtggtgtt cgtcagcgaa accgagtaca cgatcgtcgg
     5221 acccgacacc gacccggacg atccgccggt cctgcatacc ttcgttccgg gcgagatgat
     5281 caccgccggc tccgcacagt tctcgatc
//
LOCUS       DATLGF010000424        11249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_153223, whole genome shotgun sequence.
ACCESSION   DATLGF010000424 DATLGF010000000
VERSION     DATLGF010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 11249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11249
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153223"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="VM406_12925"
     CDS             complement(<1..924)
                     /locus_tag="VM406_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HVL76910.1"
                     /translation="MAFQEKQLPPDAPLSQKALNWVDSRFPLTPTWKAHLSEYYAPKN
                     FNFWYAFGSLALLVLVIQIVTGIFLVMHYKPDANLAFASVEYIMRDVPWGWLVRYMHS
                     TGASAFFVVVYLHMLRGLLYGSYRKPRELVWLFGVAIFLCLMAEAFFGYLLPWGQMSY
                     WGAQVIVNLFGAIPFIGPDLSLWIRGDYVVSDATLNRFFSFHVIAIPLVLLGLVVAHI
                     IALHEVGSNNPDGIEIKEKVDANGKPLDGIPFHPYYSVHDVYVAAVFLLLFSAVVFFA
                     PEMGGYFLEYNNFIPADPLKTPPHIAPVWYFT"
     gene            complement(927..1535)
                     /gene="petA"
                     /locus_tag="VM406_12930"
     CDS             complement(927..1535)
                     /gene="petA"
                     /locus_tag="VM406_12930"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HVL76911.1"
                     /translation="MSEEKQVDSARRGLLVATCAAGGVAGLATAGVFVSTFQPSERAR
                     AAGAPVEVDIAGLAPGQMVTVEWRGKPVWILRRSPEMLATLQKTEPQVADPLSQRNPD
                     ELTPEYARNPHRSRKPEYLVAVGICSHLGCSPITRLQPGAQPSLPDNWLGGFLCPCHG
                     STFDVAGRVFRNVPAPDNLEVPPHMYLSDTRIVIGRDEKGEA"
     gene            complement(1805..2572)
                     /locus_tag="VM406_12935"
     CDS             complement(1805..2572)
                     /locus_tag="VM406_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL76912.1"
                     /translation="MQAGAECANIMSPMPGKEKDMSEQALPRSIGVVLVEDDAVTRKC
                     LSRAIESEPSLRLLAAHDGAAAAMHWLEQVEPDVLLCDLGLPDGSGLDVIQTCVRLHP
                     RCDIMVVTMSSDEESVLASIEAGASGYVLKDAGQTDIAQAVLALRAGGAPMSPAIARM
                     VLARVRTGGRKPVSGASSASAAAASLTKKEAAILDLIAQGDSYSEVAKVLSVSVGTVQ
                     THIKNIYGKLSVHSRGEAVYEAHRRGWISLARPPEES"
     gene            complement(2616..3377)
                     /locus_tag="VM406_12940"
     CDS             complement(2616..3377)
                     /locus_tag="VM406_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HVL76913.1"
                     /translation="MEQKLVDRGSLAAYLEQTLDITRFRDYCPNGLQVEGRAAISSVV
                     TGVTASLALLDAAARAGADAVLVHHGYFWRGEDARVIGPKQRRLKLLLEHEINLFAYH
                     LPLDAHPEFGNNAQLARVLGLEASGRFGEGDLGWIGRAGGVSTVGELAARIEQALGRP
                     PLLIGDPAQALGPVGWCTGAAHGMLGDAIGAGASAFISGEISEPVVHLARETGVAYLS
                     CGHHATERYGIAALGDHLAQRFGLKHQFIDIPNPV"
     gene            3391..4551
                     /locus_tag="VM406_12945"
     CDS             3391..4551
                     /locus_tag="VM406_12945"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HVL76914.1"
                     /translation="MRRLWLLFAQTVTVGLAFWFIVASLKPEWIGAGGPAAARIATMP
                     IPMFEAPADLAPTQASYRDAAARAMPAVVNIFTSKGTRQQRHPFMEDPLFRRFFGEPY
                     EDRQFSLGSGVVVSPQGYVLTNNHVIETANEIEVALADGRKAVATVVGTDPETDLAVV
                     KIDLPNLPAITLGRLEQAKVGDVVLAIGNPFGVGQTVTMGIISALGRNQLGINTFENF
                     IQTDAAINPGNSGGALIDAQGNLLGINTAIYSRSGGSLGIGFAIPVTTIQTVMESIIT
                     TGQVVRGWIGVEPQDITPELAESFGIAQKGGAIIAGVLRGGPADRAGIRPGDILVAVQ
                     GKPVGDTTDMLNLIAQLTPGDKAQLTLVRRGQETAVEVTVGRRPRPRRDGRE"
     gene            4556..5227
                     /locus_tag="VM406_12950"
     CDS             4556..5227
                     /locus_tag="VM406_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HVL76915.1"
                     /translation="MHTRDLVRFLAELAENNNRAWFVMNKPRYDILRAEFLDLVTGLI
                     GRIGRFDPAVSGCDPKKALFRINRDMRFSKDKSPYKTQFSAAITASGLKKPSQGGGPA
                     YYFHIDQAGRLLVAGGEYMPPADRLRAIRQEIVENPAGFAKTMKTPALRKRYGELRDE
                     GKLARIPKGFEAHEALADVLKHKSYIVWTETDIGKQVPADLAAELAAAFKDAYPLVAW
                     LRAVR"
     gene            complement(5232..5990)
                     /gene="tatC"
                     /locus_tag="VM406_12955"
     CDS             complement(5232..5990)
                     /gene="tatC"
                     /locus_tag="VM406_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HVL76916.1"
                     /translation="MSDDQDSAAGQETFVSHLVELRNRVIKATAAILVVFLCLMPWAG
                     AIYDLLALPMMAALPEGSQMIATGVITPFLVPVKVTLLAAFIIALPVVLYQAWAFIAP
                     GLYAHEKRLVMPLVLSSSVLFMVGVAFCYYFVFGMVFKFINEFAPASIAVAPDIESYF
                     GFVMTLFIAFGLTFEVPVVVVVLVRMGVITVQKLKEVRPYVIVGAFVLAAIITPPDVI
                     SQLMLAIPLCLLYEVGIWIAPLFVRATQPAEEAG"
     gene            complement(5991..6470)
                     /gene="tatB"
                     /locus_tag="VM406_12960"
     CDS             complement(5991..6470)
                     /gene="tatB"
                     /locus_tag="VM406_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HVL76917.1"
                     /translation="MIDLGFTKLALIGVVALVVIGPERLPKVARMAGTLFGRAQRYMN
                     EVKAEVSREIELDELRKMQQEMQEAASSVERTIAKSVAEIEGDVSETLRNPLLDTVTP
                     DQLAVKAKDFRRKKLARNSAIPAWYKKQNGRKSRIISGAARVSRFRPNALRRNTSFF"
     gene            complement(6528..6734)
                     /gene="tatA"
                     /locus_tag="VM406_12965"
     CDS             complement(6528..6734)
                     /gene="tatA"
                     /locus_tag="VM406_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HVL76918.1"
                     /translation="MGSFSIWHWLIVLVVVLLVFGTKKLGTIGSDLGKAVKGFKDGVK
                     GEEEQSSETAAKPTIDVEAKEKKG"
     gene            complement(6784..7155)
                     /locus_tag="VM406_12970"
     CDS             complement(6784..7155)
                     /locus_tag="VM406_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HVL76919.1"
                     /translation="MTDCIFCKIAAKQIPSKTVYEDDDLIAFHDINPAAPVHVLIVPK
                     QHIATLADCTEEHAGLLGKMMLLAPQLARELGCEYAMDSECGPRGGFKTQFNTGPDGG
                     QEVYHLHLHLIGGPRPWRGQR"
     gene            complement(7171..7512)
                     /locus_tag="VM406_12975"
     CDS             complement(7171..7512)
                     /locus_tag="VM406_12975"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HVL76920.1"
                     /translation="MSETLKRLSDVIESRKPSAGGDPDTSYVARLFSKGDDAILKKIG
                     EEATELVMAAKDARAAGDPAKLVYECADLWFHSVVLLAQFGLKPDDVLAELARREGIS
                     GIEEKAARSSN"
     gene            complement(7541..7939)
                     /gene="hisI"
                     /locus_tag="VM406_12980"
     CDS             complement(7541..7939)
                     /gene="hisI"
                     /locus_tag="VM406_12980"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HVL76921.1"
                     /translation="MSAAWLDKVKWDEHGLVPVIAQEAATGDVLMFAWMNRDALAKTL
                     ELGEAVYWSRSRRKLWHKGEESGHTQKVRDIRLDCDEDVVLLKVEQAGGIACHTGRHS
                     CFFQQLSGSGADAEWKAVEPVLKDPERIYK"
     gene            complement(7936..8700)
                     /gene="hisF"
                     /locus_tag="VM406_12985"
     CDS             complement(7936..8700)
                     /gene="hisF"
                     /locus_tag="VM406_12985"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HVL76922.1"
                     /translation="MSLAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDDQG
                     ADELTFLDITATSDGRDLILSIIEAVASQVFIPLTVGGGVRTVEDVRRLLNAGADKIS
                     VNSTAVANPQMVADAAQKYGSQCIVVAIDAKKSAEGRWEVFTHGGRKATGLDVVEWAC
                     KMESLGVGEILLTSMDRDGTKSGFDLALTRAVSDAVSIPVIASGGVGGLQDLADGITI
                     GRADAVLAASIFHYGQHTVQEAKQFMAGQGIPMRLA"
     gene            complement(8697..9479)
                     /gene="hisA"
                     /locus_tag="VM406_12990"
     CDS             complement(8697..9479)
                     /gene="hisA"
                     /locus_tag="VM406_12990"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HVL76923.1"
                     /translation="MLLIPAIDLKDGHCVRLKQGDMDQATVFSDDPGRMARHWLERGA
                     RRLHLVDLNGAFAGKPKNESAVKAIIQAVRDFAAANDTEEIPIQLGGGIRDLDTIERY
                     LDDGLSYIIIGTAAVKNPGFLHDACSAFPGQIIVGLDAKDGKVATDGWSKLSGHEVID
                     LAKKFEDYGCEAIVYTDIGRDGMMAGVNIEATVKLAQSVAIPVIASGGVHTIKDVEAL
                     CEVEEEGIEGVICGRSIYEGTLDLRAAQARADELAGLDTAEE"
     gene            complement(9509..10147)
                     /gene="hisH"
                     /locus_tag="VM406_12995"
     CDS             complement(9509..10147)
                     /gene="hisH"
                     /locus_tag="VM406_12995"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HVL76924.1"
                     /translation="MNKIVVVDYGMGNLRSVAQALRHVAPEADVRISGELAEVRAADR
                     LVLPGQGAMPDCMRSLRESGLLDAVVEASRTKPLLGVCVGEQMLFERSEEGDTPGLGL
                     LPGRVVRFRLDGLTQVDGSRFKVPQMGWNRVYQTMSHALWHGIPDESYFYFVHSYYAV
                     PDDSRHTAGETDYGARFACAVAKDNIFATQFHPEKSAAAGLQLYKNFVSWNP"
     gene            complement(10184..10780)
                     /gene="hisB"
                     /locus_tag="VM406_13000"
     CDS             complement(10184..10780)
                     /gene="hisB"
                     /locus_tag="VM406_13000"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HVL76925.1"
                     /translation="MSTARVAEVIRNTNETQIRVVINLDGTGRQKLDTGVPFLDHMLD
                     QIARHGLIDLDIEAKGDLHIDAHHTVEDVGITLGQAVARAMGDKKGIRRYGHAYVPLD
                     EALSRVVVDFSGRPGLEFHVPFTRAMIGGFDVDLTREFFQGFVNHALVTLHVDNLRGT
                     NAHHQCETVFKAFGRALRMAAELDPRAAGTIPSTKGSL"
     gene            complement(10789..>11249)
                     /locus_tag="VM406_13005"
     CDS             complement(10789..>11249)
                     /locus_tag="VM406_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVL76926.1"
                     /translation="RTVSKLGLAGIRLGYMSASAPLLAELEKVRPPYNVNVLTQAAAE
                     FVLDHVEVLDEQAALLRRERERLAAELSALPGIFVFPSAANFLLVRIDAPNPDTDAVN
                     AALLERRVIVKNVGKMHPLLQNCLRITVSTPEENRAFLEAFKAAIAPPTT"
BASE COUNT         2089 a         3585 c         3552 g         2023 t
ORIGIN      
        1 cgtgaagtac cagaccggcg cgatgtgcgg cggcgtcttc agcgggtcgg ccgggatgaa
       61 gttgttgtac tcgaggaagt agccgcccat ctccggcgcg aagaacacaa ccgccgagaa
      121 cagcagcaga aacaccgcgg ccacgtacac gtcatgcacc gagtagtacg ggtggaacgg
      181 aatgccgtcg agcggcttgc cgttggcgtc aaccttttcc ttgatctcga tgccgtccgg
      241 gttgttcgaa cccacttcgt gcagcgcgat gatgtgcgcg acgaccaggc cgagcagcac
      301 cagcggaatc gcgatcacgt ggaacgagaa gaagcggttc agcgtcgcat ccgacaccac
      361 gtagtcgccg cggatccaca gggacaggtc cggaccgatg aacgggatcg cgccgaacag
      421 gttcacgatc acctgggcgc cccaatacga catctggccc cacggaagca ggtagccgaa
      481 gaaggcttcg gccatcaggc acaggaagat cgccacgccg aacagccaga ccagctcacg
      541 cggcttgcgg taggaaccgt agagcaggcc gcgcagcatg tgcaggtaga ccacgacgaa
      601 gaaggccgag gcgccggtcg agtgcatgta gcgcaccagc cagccccagg gcacatcgcg
      661 catgatgtac tcgaccgacg cgaacgccag gttcgcatcc ggcttgtagt gcatgaccag
      721 gaagatgccg gtcacgatct ggatcacgag caccagcagc gcgagcgagc cgaacgcata
      781 ccagaagttg aagttcttcg gcgcgtagta ctcggacagg tgcgccttcc aggtcggggt
      841 gagcgggaag cgcgaatcga cccagttcag ggccttctgc gacagcggtg cgtcgggagg
      901 gagttgtttt tcctggaatg ccatgatcag gcctcgcctt tctcatcgcg gccgatcacg
      961 atgcgggtgt cgctcaggta catgtggggc ggaacctcga ggttgtccgg cgccgggacg
     1021 tttcggaaca cgcggccggc gacatcgaaa gtggaaccgt ggcaggggca gaggaagcct
     1081 cccagccagt tgtcaggcag cgacggctgc gcgccgggct gcagacgagt gatcggcgag
     1141 caacccaaat gggagcagat gcccaccgcc accaggtatt ccggcttgcg cgagcggtgc
     1201 gggttgcgcg catactcggg agtcagctca tcggggttgc gctgcgaaag cgggtcggcg
     1261 acttgcggct cggtcttttg cagcgtggcc agcatctccg gcgaacgcct cagaatccat
     1321 accggcttgc cgcgccactc gaccgtcacc atctgaccgg gtgcaaggcc agcgatgtcc
     1381 acttcgaccg gcgcgccggc ggcacgagcg cgctcggacg gctggaaggt ggacacaaac
     1441 acgcccgcag tggccaaccc agccacgcca cctgccgcgc atgtcgcgac aagcaagcca
     1501 cgtcgagcgg aatcgacctg cttttcttca ctcataccaa ccccaatagt aaataagcgt
     1561 agtaacgcga aacttctagc aaccttagat tataactgat tcagcacatg tttccctagg
     1621 gtgtttgctg gaagaaagca cgaatttgag gtccggatgc gggatcggcg ccgaccggca
     1681 tccagcgccg tgtgcgtaac cgagcatcac gggcgacagg gccaacgtct ggggaatgca
     1741 gtcgccaccc gcgagaaaag gcggccggac tggggctgca ggcgggccgg aacggccgcc
     1801 cgcgtcagga ttcttcgggg gggcgcgcca ggctgatcca gccccggcga tgcgcctcat
     1861 aaacggcctc tccgcgcgaa tgcacggaaa gcttgccgta aatattcttg atgtgagtct
     1921 ggacggtccc gaccgacact gacaacacct tggcgacctc gctgtaactg tcgccctgtg
     1981 caatgagatc gagaatggcc gcctccttct tcgtcaggct tgccgccgcg gcgctcgcgg
     2041 acgacgcccc tgaaaccggc ttcctgccgc cggtacgcac acgcgcaagc accatgcgcg
     2101 cgatcgccgg actcatcggc gctccgccgg cccgcagggc gagcaccgct tgcgcgatat
     2161 cggtctgccc ggcgtccttc agcacgtagc cggaggcgcc ggcctcgatt gaagcgagca
     2221 cgctttcctc gtcactcgac atcgtgacga ccatgatgtc gcagcgcgga tggagccgga
     2281 cgcacgtctg tatgacgtcg agtccgcttc cgtccggaag gccgagatcg cacagaagca
     2341 catcaggctc tacctgctcc agccaatgca tggccgcggc cgccccgtcg tgcgcggcaa
     2401 gcaggcgcag cgaaggttcg gattcgatcg cgcgcgacag gcactttcgg gttaccgcat
     2461 catcctcgac gagcacgact ccgatcgacc tgggcaacgc ttgttcggac atgtcttttt
     2521 ctttccccgg cattggcgac atgatattgg cacactctgc gcctgcttgc atgaatgtcg
     2581 caaccgaagg atgcgctacg gcgcttgcac ggcgcttaca cgggattcgg aatgtcgatg
     2641 aactggtgct tgagtccgaa acgttgcgcc aggtgatcgc cgagcgccgc aatgccgtag
     2701 cgctcggtgg catggtgccc gcaggagaga taggccacgc cggtctcgcg cgcaagatgg
     2761 acgaccggct ccgagatctc gccgctgatg aaggcactgg cgccggcgcc aatggcgtcc
     2821 cccagcatgc cgtgcgcggc gcccgtgcac catccgacag gcccgagagc ctgcgccgga
     2881 tcgccgatga gcagaggggg ccgcccgagc gcctgctcga tgcgcgcagc cagctcacct
     2941 acggtggaga cgcctccggc cctgccaatc catccgaggt ccccttcacc gaagcggccg
     3001 ctcgcctcca gccccagcac gcgtgctagc tgggcgttgt tgccgaactc cggatgcgcg
     3061 tccagcggca agtgatatgc gaacaggtta atttcatgct ccagcaacag cttcaggcgc
     3121 cgctgcttcg ggccgatcac gcgggcatcc tcgccgcgcc agaaatagcc atggtggaca
     3181 aggacggcgt cggcgccggc tcgggccgcc gcatcgagca aggcaaggct cgcagtcacg
     3241 cccgtgacga ccgaggaaat tgccgcccgc ccctcaacct ggagtccgtt tgggcaatag
     3301 tcgcgaaaac gggtaatatc gagcgtttgc tcaagatagg ctgccaagct gccacgatcg
     3361 acgagtttct gttccattct ttctgccttt atgcgacgcc tctggctctt gtttgcacaa
     3421 accgtaactg tcggtttggc gttctggttc attgtagcga gcctgaagcc cgagtggatc
     3481 ggagcgggcg gacctgccgc cgcgcgcatc gcgacgatgc ccattccgat gttcgaggcg
     3541 ccggccgatc tcgcccccac ccaggcgagc taccgggacg ccgctgcccg cgccatgccg
     3601 gccgtggtca acatcttcac gagcaagggc acccgccagc agcgccatcc gttcatggag
     3661 gatccgctgt tccggcgctt cttcggcgag ccgtacgaag ataggcagtt cagccttggc
     3721 tccggcgtgg tggtcagccc gcagggctat gtgctcacca acaaccacgt gatcgagacc
     3781 gcgaacgaaa tcgaagtcgc gctcgcggac ggacgcaagg ccgtcgccac cgtggtcggg
     3841 accgatcccg agaccgatct tgccgtcgtg aagatcgacc tgccgaacct tcctgcaatt
     3901 acccttggcc ggcttgaaca ggcgaaggtc ggggatgtgg tgctcgcaat cgggaatcct
     3961 ttcggtgtcg gccagacggt gacgatgggg atcatttccg cgctcgggcg caatcagctc
     4021 ggcatcaata ctttcgaaaa cttcatccag actgacgccg ccatcaatcc gggcaactcg
     4081 ggtggagcgc tgatcgacgc gcaggggaat cttctcggta tcaataccgc aatttattcc
     4141 cgcagcgggg gctctcttgg catcgggttt gccattcccg tcacgaccat ccagaccgtg
     4201 atggagtcca tcatcacgac cggccaggtg gtgcgcggct ggatcggcgt cgagcctcag
     4261 gacatcacgc cggaactggc ggagagcttc ggcatcgcgc agaagggcgg cgcgatcatc
     4321 gccggcgtgc tgcgcggggg gccggcggac cgtgccggca tccgccccgg cgacatcctc
     4381 gtcgcggtcc agggcaagcc ggtgggagac accaccgaca tgctcaatct gatcgctcaa
     4441 ttgacgcccg gcgataaggc gcagttgacc ctggtgcggc gcggtcagga aaccgcggtt
     4501 gaagtgacgg tcggacggcg tccgcgccct cgccgcgacg gcagggagtg atccgatgca
     4561 cacgcgcgat ctggttcgtt ttcttgccga acttgcagag aacaacaatc gcgcgtggtt
     4621 cgtgatgaac aagccgcgct acgacatcct gcgcgcggag tttctcgatc tcgtcaccgg
     4681 cctgatcggg aggattggcc gtttcgatcc ggccgtatcc ggctgcgatc cgaagaaggc
     4741 gctgtttcgc atcaaccgcg acatgcgctt ctcgaaggac aagagtccgt acaagacgca
     4801 gttctcggcg gcgatcaccg ccagcggcct gaagaagccg agccagggcg gggggccggc
     4861 ctactacttc catatcgatc aggccggccg gctgctcgtc gccgggggcg agtacatgcc
     4921 tcctgccgat cggctgcgag caatccggca ggaaatcgtc gaaaacccgg ccggctttgc
     4981 caagacgatg aaaacgccgg cgctgcgcaa gcgctacggc gaactgcgcg acgaaggcaa
     5041 gctggcccgc attcccaagg gattcgaagc gcacgaagcg ctggccgacg tcctgaaaca
     5101 caagagctac atcgtgtgga cggaaaccga tatcggcaag caggtcccgg ctgacctggc
     5161 ggcagaactg gccgccgcgt tcaaggatgc ctatccgctg gttgcctggc tgagggcggt
     5221 ccggtaggtc ttcagcccgc ctcttcggcg ggctgggtcg cacgcacgaa cagcggcgca
     5281 atccagatac ccacttcata gagcaggcac agtggaattg cgagcatcaa ctggctgatc
     5341 acgtccggcg gcgtgatgat ggcggcaagc acgaaggcgc cgacgattac atagggccgc
     5401 acttccttga gcttctgcac cgtaatcacc cccatgcgca ccagcacgac caccaccacc
     5461 ggcacttcga aggtcagtcc gaaggcgatg aaaagcgtca tcacgaagcc gaaatagctt
     5521 tcgatgtccg gcgcaaccgc gatcgaggcg ggtgcgaact cgttgatgaa cttgaacacc
     5581 atcccgaaca cgaagtagta gcagaacgcc acaccgacca tgaacaaaac ggaagacgag
     5641 agaaccagcg gcatcaccag ccgcttttca tgcgcgtaca ggccaggcgc aatgaacgcc
     5701 catgcctggt agagcacgac cggcagcgcg atgatgaagg cagccagcag cgtcaccttc
     5761 accggcacga ggaagggcgt gatgacaccg gtggcgatca tctggctgcc ttccggcagc
     5821 gccgccatca tcggcaacgc caacaggtcg tagattgcac cggcccatgg cattaaacac
     5881 aggaacacca cgaggatcgc ggcagtggcc ttgatcacgc ggttgcgcag ctcgaccagg
     5941 tgggagacga aggtttcctg cccggctgca gaatcttgat cgtcgctcat ttagaagaat
     6001 gaagtgttgc gtctgagcgc attgggcctg aaccgggaca cgcgcgcggc gccggagatg
     6061 atgcgcgact tgcgaccatt ctgcttcttg taccaggcag gaatcgccga attgcgtgca
     6121 agtttcttgc ggcggaaatc cttggccttg acggccagct ggtccggggt gacggtgtcg
     6181 agcaaggggt tgcgcaaggt ctcgctcacg tcgccctcga tctcggcgac gcttttcgcg
     6241 atcgtgcgct cgacgctgct ggccgcctcc tgcatttcct gctgcatctt gcgcagctcg
     6301 tcgagctcga tctcgcggct gacttcggcc ttgacctcgt tcatgtaacg ctgggcgcgc
     6361 ccgaacaggg taccagccat gcgcgcgacc ttgggcaggc gttcggggcc gatgacgacc
     6421 agcgcaacca ccccgatcag cgcgagtttc gtgaaaccta gatcaatcat gatgaacccg
     6481 catggcgcgc gtcaatgcag ccgcgcgcgc atctgggaag cgttgcatca gcccttcttt
     6541 tccttcgctt cgacgtcgat ggtcggcttg gccgcagtct cggagctctg ctcctcctcg
     6601 cccttgacgc catccttgaa gcccttgact gccttgccca gatccgagcc gatcgtgccc
     6661 agtttcttgg tgccgaatac cagcaagacg accaccagca cgatcaacca gtgccagatg
     6721 ctgaacgaac ccattacctt ctccttgtcg gacgaatatg tccgctaaat caaataatta
     6781 ctactaccgc tgaccgcgcc agggacgcgg gccgccgatc aggtgcaagt gcagatggta
     6841 cacctcttga ccgccatcag gtccggtatt gaactgggtt ttgaagcctc cgcgagggcc
     6901 gcactcgcta tccatggcgt actcgcaacc caattcgcgc gcgagttgcg gagccagcaa
     6961 catcatcttg cccaggaggc ccgcatgttc ctcggtgcag tcggcgagcg tggcgatatg
     7021 ctgcttcggc acgatcagga catggaccgg tgccgcgggg ttgatgtcgt gaaacgcaat
     7081 cagatcatcg tcctcgtaca cggtcttcga tggaatctgt ttcgcagcga tcttgcagaa
     7141 aatgcagtcg gtcatcggtg ctcctctcgt tcagttggaa ctgcgtgcag ctttctcttc
     7201 gataccggag atcccctcgc ggcgagcgag ttcggccagc acatcatcgg gcttcaagcc
     7261 gaactgcgcg agcagcacga ccgagtggaa ccacaggtcc gcgcattcat agacgagctt
     7321 ggcgggatcg ccggcggcgc gggcgtcctt ggccgccatc acgagttcgg tggcctcttc
     7381 gccgatcttc ttcaggatcg cgtcgtctcc cttcgaaaac aggcgcgcca cataggaggt
     7441 gtcgggatcg ccgccggcag acggcttgcg tgattcgatg acgtcggaaa gacgtttgag
     7501 ggtttcgctc atcggcatag gcggcaggct cgccgcgtcc ttacttgtag attcgctcgg
     7561 ggtccttgag gacgggttcg accgccttcc attcggcatc tgcgccgctg ccgctcagct
     7621 gctggaagaa gcaggaatgg cggccggtat ggcaggcgat gccgccggcc tgctcgacct
     7681 tcagcagcac cacatcctcg tcgcagtcga ggcggatgtc gcgcaccttc tgggtgtggc
     7741 cggactcctc gcccttgtgc cacagcttcc tgcgggaacg gctccagtac acggcttcac
     7801 caagctcgag ggttttcgcg agcgcgtctc gattcatcca ggcaaacatc agcacgtcgc
     7861 cggtggccgc ttcctgcgcg atcaccggaa cgagtccgtg ctcgtcccac ttgaccttgt
     7921 cgagccacgc ggcgctcatg ccagcctcat cgggatgccc tgccccgcca tgaattgctt
     7981 ggcttcctgc acggtgtgct ggccgtagtg gaagatgctg gccgcgagca ccgcgtcggc
     8041 gcggccgatc gtgatgccgt cggccaggtc ctgcaggccg ccgacgccgc ccgaggcgat
     8101 caccggaatc gataccgcgt cggataccgc gcgcgtcagt gcgaggtcga agccgctctt
     8161 ggtgccgtcg cggtccatgc tggtcagcag gatctcgccg acgcccaggc tctccatctt
     8221 gcacgcccat tcgaccacgt ccagtcctgt cgccttgcgt ccaccatggg tgaacacttc
     8281 ccagcgccct tccgcgctct tcttggcatc gatcgccacg acgatgcact gcgagccgta
     8341 cttctgcgcg gcgtccgcca ccatctgcgg attcgccacc gcggtgctgt tgacgctgat
     8401 cttgtccgcg ccggcgttca gcaggcgccg cacatcctcg acggtgcgca cgccgccacc
     8461 gacggtgagc ggaatgaaga cctgggaggc gaccgcctcg atgatgctca ggatcaggtc
     8521 gcgtccgtcc gaggtcgcgg tgatgtcgag aaaggtcagt tcgtcggcac cctggtcgtc
     8581 gtagcggcgc gcgatctcga ccggatcgcc ggcatcgcgc agctcgacga aattgacgcc
     8641 cttgacaacg cgaccggcgg tcacgtcgag acaggggatg atgcgtttgg caagactcat
     8701 tcttccgccg tgtccagtcc cgccagctcg tccgcgcgtg cctgggccgc gcgcaggtcc
     8761 agcgtgcctt catagatcga gcggccgcag atcacgccct cgatgccctc ttcctcgacc
     8821 tcacagagcg cttcgacatc cttgatcgtg tgcacgccgc cggatgcgat gaccgggatg
     8881 gccacgctct gcgcaagctt gaccgtggcc tcgatgttga cgccggccat catgccgtcg
     8941 cggccgatgt cggtgtagac gatggcttcg cagccgtagt cctcgaattt cttggccaag
     9001 tcgatcactt catggccgga aagcttgctc cagccatcgg ttgcgacctt gccgtccttg
     9061 gcgtccaggc cgacgatgat ctggccggga aacgcgctac acgcatcgtg caggaatccg
     9121 ggattcttta ccgccgcggt gccgatgatg atgtaggaca gcccgtcgtc caggtagcgc
     9181 tcgatcgtat cgaggtcgcg gatgccgccg cccagttgaa tcgggatttc ctcggtgtcg
     9241 ttcgcggcag cgaaatcacg cacggcctgg atgatcgcct tgaccgccga ctcgtttttc
     9301 ggcttgccgg cgaacgcacc gttcaggtcg accaggtgca ggcgccgcgc gccccgctcg
     9361 agccaatggc gcgccatccg gcccggatcg tcggagaaca cggtggcttg atccatgtcg
     9421 ccttgtttca ggcgtacgca atgaccgtcc ttgaggtcga tggcaggtat gagcagcatg
     9481 gccttggttg tggtggttgg acgaagaact aggggttcca ggagacaaaa ttcttgtaga
     9541 gctgcaaacc ggccgcagcg cttttttcag ggtgaaactg ggtcgcaaag atattgtctt
     9601 tcgccacggc gcaggcaaag cgcgcaccgt aatcggtttc ccccgccgtg tgccggctgt
     9661 catcgggcac cgcgtagtag ctgtgcacga aatagaaata gctttcgtcg gggatgccgt
     9721 gccacagcgc gtgggacatt gtctgatata cccggttcca gcccatttgc ggcactttga
     9781 agcgtgatcc gtccacctgt gtaaggccat cgagacgaaa gcgcacgacc ctgccgggca
     9841 gcaggccgag gcccggcgta tcgccctcct cgctgcgctc gaacagcatc tgctcgccga
     9901 cgcacacacc cagcagcggc ttggtgcgac tggcctcgac caccgcgtcc agcaggccgg
     9961 attcgcgcag gctgcgcatg cagtcgggca tcgcgccctg gcccggcagc acgaggcggt
    10021 ccgccgcgcg cacctcggcc agttcaccgg aaatgcgcac gtcggcctca ggggcgacat
    10081 ggcgcagggc ttgcgcaacc gagcgcaggt tgcccatgcc gtagtccaca acaacgatct
    10141 tgttcatttc gtcagaaaaa acgcggtgcg aaaccgccag tcctcagagg ctgcccttgg
    10201 tcgacggaat ggtgccggcc gcacgcggat ccagctccgc ggccatgcgc agcgcgcggc
    10261 cgaaggcctt gaatacggtc tcgcactggt ggtgcgcatt cgtgccgcgc aaattatcca
    10321 catgcaatgt gaccagggca tgattgacga agccctggaa gaattcgcgg gtgaggtcga
    10381 cgtcgaagcc gccgatcatt gcgcgggtga acgggacgtg gaactccagg ccgggacggc
    10441 cggagaaatc gaccaccacg cgcgagagcg cctcgtcgag cggcacatag gcgtggccgt
    10501 agcggcggat gcccttcttg tcgcccatcg cccgcgccac ggcctggccg agcgtgatgc
    10561 caacgtcttc cacggtgtga tgcgcatcga tgtgaagatc gcccttggct tcgatgtcga
    10621 ggtcgatcag cccgtggcgg gcgatctggt cgagcatgtg gtcgaggaag gggacgccgg
    10681 tatcgagctt ctgccggccg gtgccgtcga ggttgatcac gacgcggatc tgcgtctcgt
    10741 tggtgttgcg gatgacctct gcaacgcggg cggtggacat ggtgttcgtc aggtggttgg
    10801 gggagcgatc gcggccttga aggcttcgag gaacgctcgg ttttcttcgg gggtgcttac
    10861 ggtgatacgc aggcaatttt gcagcaatgg gtgcatttta ccgacatttt tgacgatcac
    10921 cctgcgctcc aggagtgccg cgttgaccgc gtcggtgtcg ggattcggcg catcgatacg
    10981 caccagcagg aaatttgcgg ccgagggaaa cacgaaaatg ccgggcaagg ccgacagctc
    11041 ggccgcgagc cgctcgcgct cgcgccgcag cagcgccgcc tgctcgtcga gcacttcgac
    11101 gtggtcgagc acgaactcgg ccgcggcctg ggtgagcacg ttgacgttgt agggcgggcg
    11161 cactttttcg agttccgcca gcagtggtgc gcttgcgctc atgtagccga gccggatgcc
    11221 ggccaggccg agcttggaca ccgtgcgca
//
LOCUS       DATLGF010000425         3451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_76761,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000425 DATLGF010000000
VERSION     DATLGF010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76761"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VM406_13010"
     CDS             complement(<1..636)
                     /locus_tag="VM406_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HVL76927.1"
                     /translation="MSTTAVDHAHDHSHDHAHDHPHGWRRWVFATNHKDIGTLYLWFS
                     FVMLLSGGVLALGIRAELFQPGLQLMQPEFFNQLTTMHGLIMVFGAIMPAFVGFANWM
                     VPLQIGASDMAFARMNNFSFWLLPPAAILLAASFLVPGGATAAGWTLYAPLTVQMGPG
                     MDMAIFAMHIMGASSIMGSINIIVTILNMRAPGMTLMKMPMFAWTWLITAYL"
     gene            complement(655..1812)
                     /gene="coxB"
                     /locus_tag="VM406_13015"
     CDS             complement(655..1812)
                     /gene="coxB"
                     /locus_tag="VM406_13015"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HVL76928.1"
                     /translation="MKLAKRLQSLMLGASLLAAGVPTWAQVPDSPGGPAVRQMTFHPP
                     VTKIAEEIYSLHNMMLVICLAIFLAVFGVMFYSIWKHRKSVGHKPASFHESTAVEIAW
                     TVVPFLIVIGMALPATKTVVAMKDTSNADITIKATGMQWKWGYDYLKGEGEGISFLQA
                     MSTPREQIVDAAARGTNYLIEVDNPVVVPVNKKIRIITTANDVIHAWGIPSFGVKQDA
                     IPGFVRDTWFRAERTGVYRGHCYELCGKDHAFMPIEVHVVTDAEYTQWVEAKQKEMAA
                     MADDPTKTWTIDELVQRGAKVYAANCVACHQANGQGVPGAFPALAGSANVNGPMQEQV
                     QVVMNGRRGMPSFKQLSDTELAAVITYTRNSWGNQAQENIIQPAQVQAARK"
     gene            complement(1827..2288)
                     /locus_tag="VM406_13020"
     CDS             complement(1827..2288)
                     /locus_tag="VM406_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HVL76929.1"
                     /translation="MAAREWVLKRNCSLTPRQLGIALLVLCALSLLVATVFALHGFWL
                     VLCFSVLELSAVTLAFLLYARHAADRERIALDESCLLVELVESERVTQYKLDPRYTQV
                     EPPRAGKPLVGLRARGTRVEVGRFATETRRRELARELRSALASAGQTGFQF"
     gene            complement(2549..>3451)
                     /locus_tag="VM406_13025"
     CDS             complement(2549..>3451)
                     /locus_tag="VM406_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVL76930.1"
                     /translation="IDMQRVRELFRAPERVAQADFLRREVAARMHERLMLIKATPERV
                     LDAGCGTGADLVHLQQRYPSAQVIGLDGVPAMLAAAARREEQAASAVQRLLHRLAPAA
                     LRPGVPGAALVCSDFARLPLAAGSVDMLWSNLALHWHPHPDMVFSEWRRVLRVEGLLM
                     FSGFGPSTFKEVRAAFSVLDDAPHTLSFVDMHDLGDMLIHGGFSTPVMDMETITITYD
                     STARLLAELRAWGGNPLSTRRRGLIGRRAWQRVLDAFEVQRGADGKLHLTCEVVYGHA
                     FRPAPRVTAAGESIVRFDPPRPRR"
BASE COUNT          680 a         1071 c         1100 g          600 t
ORIGIN      
        1 caggtaggcg gtgatgagcc aggtccacgc gaacatcggc atcttcatca gcgtcatgcc
       61 gggtgcgcgc atgttcagga tcgtgacgat gatgttgatc gagcccatga tcgacgaggc
      121 gcccatgatg tgcatcgcga agatcgccat gtccatgccg ggccccatct ggaccgtcag
      181 cggcgcatac agcgtccagc cggctgcggt tgcaccgccg ggaaccagga aggatgcggc
      241 caacaggatc gccgccggcg gcagcagcca gaacgagaag ttgttcatgc gcgcgaacgc
      301 catgtcggag gcgccgatct gcagcggcac catccagttc gcgaagccga cgaaggccgg
      361 catgatcgcg ccgaacacca tgatcagacc gtgcatggtg gtcagctgat tgaagaactc
      421 cggctgcatc aactgcaggc cgggctggaa cagctcggcg cggatcccga gggcgagcac
      481 gccgccggaa agcagcatga cgaacgagaa ccacaggtac agggtaccga tgtccttgtg
      541 gttggtcgca aagacccagc gacgccagcc gtgcggatgg tcgtgcgcgt gatcgtgaga
      601 atgatcgtgg gcgtgatcga ctgcggtggt gctcatctca attctccaat tcaattactt
      661 gcgcgccgcc tgcacttggg ctggttgaat gatgttttcc tgtgcctgat tgccccagga
      721 attgcgggtg taggtgatca ccgccgcgag ttcggtatcg ctgagctgct tgaaggacgg
      781 catgccgcgg cgaccattca tcacgacttg cacctgttcc tgcatcgggc cgttcacgtt
      841 ggcagatcct gccagcgcag ggaaggcacc gggcacgccc tggccgttag cctggtggca
      901 agccacgcag ttggcggcat agaccttcgc gccgcgctgc accagctcgt cgatggtcca
      961 ggtcttggtc ggatcgtcgg ccatggctgc catttccttc tgcttggcct cgacccactg
     1021 ggtgtactcg gcatcggtca cgacatgcac ttcgatgggc atgaacgcat ggtccttgcc
     1081 gcacagctca tagcagtggc cgcggtagac gccggtgcgt tcggcgcgga accatgtgtc
     1141 gcgcacgaag ccggggattg cgtcctgctt gacgccgaag gacggaatgc cccatgcatg
     1201 gatcacgtcg ttggcggtgg taatgatgcg gattttcttg ttgaccggca cgaccacggg
     1261 attgtcgacc tcgatcagat agttcgtacc cctggctgca gcgtcgacga tctgttcgcg
     1321 cggggtggac atggcctgca ggaaggaaat gccttcacct tcgcccttga ggtagtcata
     1381 gccccacttc cactgcatgc cggtcgcctt gatggtgatg tcggcgttgg aggtgtcctt
     1441 catcgcaaca acggtcttgg tggcgggcaa cgccatgccg atgacgatga ggaacgggac
     1501 aaccgtccag gcgatttcga cggcggtgct ttcatggaag gacgccggct tgtggccgac
     1561 ggacttgcga tgcttccaga tcgaatagaa catgacaccg aacacggcaa ggaagatcgc
     1621 caggcagatc accagcatca tgttgtggag ggagtaaatc tcttctgcga tcttcgtgac
     1681 cggcggatgg aaagtcatct gccgcaccgc gggaccgccc gggctatctg gcacctgcgc
     1741 ccatgtcggc actccggccg ccaggagcga cgcaccgagc atcaaggatt gaaggcgctt
     1801 cgcaagtttc ataatttcct caaaacctaa aattggaatc ccgtctgtcc tgccgacgcg
     1861 agcgcgctgc gcagttcgcg cgccaactcc cgtcttcttg tttccgtcgc aaagcgcccc
     1921 acttcaaccc tggtgccgcg cgcccgcagc cccaccaagg gcttgccggc cctcggcggc
     1981 tcgacttgcg tgtagcgcgg atcgagcttg tattgcgtga cccgctcgct ctcgaccagc
     2041 tcgaccagca ggcagctttc atcgagagca atgcgctccc ggtccgcggc atggcgagca
     2101 tacagcaaaa aagcaagcgt cactgctgac aactccagca cggaaaagca gagcacgagc
     2161 cagaatccgt gcagcgcgaa caccgtggcg acaaggagcg aaagcgcaca caggacaagc
     2221 aaggcaatac ccagctgccg cggcgtcagc gagcagttgc gtttcagcac ccactcacgc
     2281 gcggccatga tgcacacctt gcaggccggt tttggacgca atatggccgc tgtgcaaaag
     2341 accggaagta taagacttaa cttggcgcgg tatcaagtgg aagtctgtcc tacaagcaag
     2401 ggtaaaaggg atggtccggc cacccggaaa tggcggaaag cgttgcgcct tcggcaattc
     2461 cttgacccgg gtcagatagc tgcggaccat cggtgtgtag gtgtttgtcc ggctccctgt
     2521 cgaggaaggc cgcgtgaaga tgccgccgtc agcggcgcgg gcgaggcggg tcgaagcgca
     2581 cgattgactc gcccgccgcg gtgacccgcg gagccgggcg aaacgcgtgg ccatagacga
     2641 cctcgcaggt cagatgcaac ttgccgtcgg cgccgcgctg cacctcgaag gcgtcgagca
     2701 cgcgttgcca tgcccgcctg ccgatcaacc cgcgacgccg tgtggacagc gggttgccgc
     2761 cccaggcgcg cagttctgcc agcaatctcg ccgtagagtc gtaggtgatg gtaatggttt
     2821 ccatatccat caccggcgtt gagaaacctc catgaatgag catgtcgccc agatcgtgca
     2881 tatcgacgaa tgaaagcgtg tgcggtgcat cgtcaagcac cgagaacgct gcccgcactt
     2941 ccttgaacgt ggagggcccg aaaccggaga acatcagcag gccctccacg cgcagcacgc
     3001 gccgccactc gctgaacacc atgtccggat gcgggtgcca gtgcagcgcg aggttggacc
     3061 acagcatgtc gacgctcccg gctgcaagcg ggagacgcgc gaagtccgaa cagaccagcg
     3121 ccgcacccgg cacgccgggg cgcagcgccg cgggcgcaag ccgatgcagc aggcgctgca
     3181 cggccgacgc cgcctgctcc tcgcggcgcg cagccgccgc gagcatggcg ggcacgccgt
     3241 ccagaccgat cacctgggcg gacggataac gctgctgcaa atggacaagg tcggcgccgg
     3301 taccgcagcc cgcgtcgagc acgcgctcgg gtgtggcctt gatcagcatg aggcgctcat
     3361 gcatgcgggc ggcaacttcg cgacgcagga aatccgcttg ggcgacgcgt tccggcgcgc
     3421 gaaacagttc gcgcacccgc tgcatgtcaa t
//
LOCUS       DATLGF010000426         3754 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_121520, whole genome shotgun sequence.
ACCESSION   DATLGF010000426 DATLGF010000000
VERSION     DATLGF010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121520"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VM406_13030"
     CDS             <1..315
                     /locus_tag="VM406_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HVL76931.1"
                     /translation="EIKELIGDSVAKVDSGYALVANAGRQMEEIVTSVKQVVAIMEEI
                     SSASVEQSAGIEEINRAIASMDEMTQQNAALVEEAAAAAAAMQQRAAKLGQEVAAFQL
                     QG"
     gene            complement(334..1194)
                     /locus_tag="VM406_13035"
     CDS             complement(334..1194)
                     /locus_tag="VM406_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVL76932.1"
                     /translation="MDPLIASFHDATTGTVTHVVHDPATRRAAVVDPVLDFDPKSGRT
                     STRSADRVIEFVRARDLHVDWILETHAHADHLSAAPYLKQELGGRIAIGEQIRTVQRV
                     FKKLFNLEATFATDGSQFDHLFADGETFSIGTLQAQALFVPGHTPADSAYRIGNAVFV
                     GDTLFMPDAGTARCDFPGGDAATLYRSIRRLLSLPPETRLYMCHDYAPGGREAAWVAT
                     VAEQRARNIHVHDGVSEEEFVRMRTERDATLEMPVLILPSIQVNVRAGELPPAEANGV
                     HYLKLPVNAL"
     gene            1271..1603
                     /locus_tag="VM406_13040"
     CDS             1271..1603
                     /locus_tag="VM406_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HVL76933.1"
                     /translation="MQDTHSPIDIALMRTAAQRASSVLRALSNEDRLLLLCQLSQGEC
                     SVSELEEALDIRQPTLSQQLAVLRGEGLVNTRRDGKRIYYSVADPQMLVLLDTLYQLY
                     CPSGEAGQ"
     gene            1600..2046
                     /locus_tag="VM406_13045"
     CDS             1600..2046
                     /locus_tag="VM406_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HVL76934.1"
                     /translation="MNIDWTNFTPVPALAGGVLIGVAAAMLVLFNGRIAGISGIIGGV
                     LQRSRPPGEAGWRLAFLAGLLVAPLLAAAVTGLPDIRIDAGAGTLILAGLLVGLGTRY
                     GSGCTSGHGVCGLSRFSPRSLAATMLFMAAGFVTVYVVRHLFGGLG"
     gene            2043..2483
                     /locus_tag="VM406_13050"
     CDS             2043..2483
                     /locus_tag="VM406_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010119174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HVL76935.1"
                     /translation="MNVIIAFLSGLVFGVGLLLAGMANPAKVLAFLDLAGAWDPSLAL
                     VMAGAIGVGTIAFSWAKRRSLSLLGLAMQLPGATDIDRRLIGGSLLFGVGWGLAGICP
                     GPAVVLIGAGVAEGWIFFGAMLAGMAAFELSRRAGDLLHAGRRT"
     gene            2749..3606
                     /locus_tag="VM406_13055"
     CDS             2749..3606
                     /locus_tag="VM406_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HVL76936.1"
                     /translation="MLYPELFKSLEQVRWDMEKDIPWDKFDASKLTEEQAQTIRMNAI
                     TEWSALPATEMFLRDNRNDSDFSAFMSIWFFEEQKHSLVLMEYLRRFRPDLVPTEEEL
                     HNVRFEFDPAPPLETLMLHFCGEIRLNHWYRCASEWHTEPVIKHIYKTISQDEARHGG
                     AYLRYMKKALNEVGDQARAAFAKIGVLMASARRTDKPLHPTNLHVNEKLFPNDTVQSR
                     LPDPNWLERWLDGQIKFDGEWEKKVVDRILHNMSLLFERTFESVQDLNRYRKEVTARL
                     GQSSVPSAA"
BASE COUNT          644 a         1168 c         1239 g          703 t
ORIGIN      
        1 gaaatcaagg agctgatcgg tgattccgtg gccaaggtcg acagcggcta tgcgctggtc
       61 gccaacgccg gccggcagat ggaagagatc gtgacttccg tcaagcaggt cgtcgccatc
      121 atggaagaga tcagctcggc cagcgtcgag cagagcgccg gcatcgagga aatcaaccgc
      181 gcgatcgcca gcatggacga gatgacgcag cagaacgcgg cgctggttga agaagccgcc
      241 gccgcggccg ccgcgatgca gcagcgcgcg gcgaaactcg gccaggaagt cgcggcgttc
      301 cagcttcagg gttgatgccg ccgccgggcg cgcctacaag gcgttgaccg gcagcttcag
      361 gtagtgcacg ccgttggcct ccgccggcgg cagttcgccc gcccggacgt tgacctgtat
      421 cgaaggcagg atcagcaccg gcatctccag tgtcgcatcg cgttcggtgc gcatgcgcac
      481 gaattcctcc tcgctcacgc catcgtgcac atggatgttg cgtgcgcgct gctccgccac
      541 tgtcgccacc cacgctgcct cgcgtccgcc gggcgcatag tcgtggcaca tgtacaggcg
      601 cgtttccggc ggaaggctca gcagccggcg gatcgagcgg tacagcgtgg cggcgtcgcc
      661 gccggggaag tcgcagcgcg ccgtgccggc atccggcatg aacagcgtgt cgccgacgaa
      721 taccgcattt ccgatccgat acgcgctgtc cgctggcgta tggccgggca cgaacaaggc
      781 ctgcgcttgc agagtgccga tcgagaatgt ctcgccatcg gcaaacaggt gatcgaactg
      841 gctgccgtcg gtcgcgaacg tcgcctccag gttgaacagc ttcttgaaca cgcgctggac
      901 ggtgcggatc tgctcgccga tcgcgatgcg gccgccgagt tcctgcttca ggtaaggggc
      961 ggcggaaagg tggtcggcgt gcgcgtgcgt ctccaggatc cagtcgacgt gcagatcgcg
     1021 ggcgcgcacg aactcgatca cccggtcggc ggaccgggtc gaggtgcgcc ccgatttcgg
     1081 atcgaaatcg agcaccgggt cgactaccgc cgcgcgtcgg gtggcgggat cgtgcacgac
     1141 gtgggtgacc gttccggtgg tggcgtcgtg aaacgaagcg atgagagggt ccatgagggt
     1201 ctcgctaaca ttatatcgat caatagaata tcatgcggta taatgaaatc aagcagattc
     1261 tggaggcttc atgcaggaca ctcattcccc gatcgacatc gccttgatgc gcacggccgc
     1321 acaacgcgcc agcagcgtgc tgcgcgcgct gtccaacgag gaccgattat tgctcctgtg
     1381 ccagttaagc cagggtgaat gctcggtctc tgaactggag gaggcgctgg acatccggca
     1441 gccgaccttg tcccagcagc ttgctgtgtt gcgcggcgag ggcctggtca acacgcgccg
     1501 cgacggcaag cgcatctact actcggtcgc cgatccgcag atgctggtgc tgctcgatac
     1561 gctgtatcag ctgtactgtc cgagcgggga ggctgggcaa tgaacatcga ctggacgaat
     1621 ttcacgcccg tgccggcgct cgccggcggc gtgctgatcg gtgtggccgc agccatgctg
     1681 gtcctgttca acggtcgcat cgccggcatc agcggcatca tcggcggcgt gttgcagcgc
     1741 agccggccgc cgggtgaggc cggctggcgc ctggccttcc tggccggact gctggtcgcg
     1801 ccgctgcttg ccgccgccgt gacggggctg ccggacatcc gcatcgacgc tggagcgggc
     1861 acgctgattc tggctggcct gctggtcggc ctcggcaccc gctacggttc cgggtgcacc
     1921 agcggccacg gcgtgtgcgg cctgtcgcgc ttctccccgc ggtcgctggc ggcgacgatg
     1981 ctcttcatgg cggccggctt cgtcacggtc tacgtcgtgc gtcatctgtt tggaggtctc
     2041 ggatgaacgt gatcatcgct ttcctctccg gactggtgtt cggcgtcggc ctgctgctgg
     2101 cgggcatggc caacccggcg aaggtgctcg ctttcctcga cctcgcgggc gcctgggacc
     2161 cttcgctggc gctggtgatg gccggtgcga ttggcgtcgg gaccatcgct ttttcatggg
     2221 cgaagcggcg cagcctgagc ctgctcggcc tggcgatgca attgcccggc gccaccgaca
     2281 tcgatcgccg cctgattggc ggcagtctgc tgttcggcgt aggttgggga ctggccggca
     2341 tatgcccggg cccggcggtc gtgctgatcg gcgccggtgt ggccgaaggc tggatcttct
     2401 tcggcgcgat gctggccggc atggcggcat tcgaactgtc tcggcgggcc ggcgacctcc
     2461 tgcacgccgg ccggcgcaca tgacattcga acttccgccg ctgctctgcg tccgactgtt
     2521 gcggaccatg ctcgtcgcgc tgcatcggtg gttcccgcgc tgtgcgtgat gttgcacttg
     2581 cgttacgttt tcatcgctgc gatttgcgcc ttttcagaag gcaggcactt gctgttgcga
     2641 agttggtctt agtgaggcga acatgcacgc ggcgaacagg gcgtgtcgca aggcggcgca
     2701 agacttctct gctagaatca aggacttatt tgtttttgaa ggtgtggaat gctgtatccg
     2761 gagctattca aatcactcga acaagttcgt tgggacatgg aaaaagacat cccctgggac
     2821 aagttcgacg cctcgaagct gaccgaagaa caggcgcaga cgattcgcat gaacgcgatc
     2881 accgagtggt ccgcgctgcc cgccacggaa atgttcctcc gggataaccg caacgacagc
     2941 gatttctccg ccttcatgtc gatctggttc ttcgaggagc agaagcactc gctggtgttg
     3001 atggaatacc tgcgccgctt ccgccccgac ctggtgccga ccgaagaaga gttgcacaac
     3061 gtgcgcttcg agttcgatcc ggcgccgccg ctggaaaccc tgatgctgca tttctgcggc
     3121 gagatccggc tgaaccactg gtaccgttgc gcgtccgagt ggcacacgga accggtcatc
     3181 aagcatatct acaagaccat cagccaggac gaagcccgtc acggcggcgc ctatctgcgc
     3241 tacatgaaga aggcgttgaa cgaagtcggc gatcaggcgc gtgcagcgtt cgcgaagatc
     3301 ggggtgctga tggcgtccgc gcgccgtacc gacaagccgc tgcatccgac caacctgcac
     3361 gtgaacgaga agctgttccc gaacgacacc gtgcaaagcc gcctgccgga tccgaactgg
     3421 ctcgagcgct ggctcgacgg ccagatcaag ttcgacggcg agtgggaaaa gaaggtggtc
     3481 gaccgcatcc tgcacaacat gtcgctgctg ttcgaacgca ctttcgagtc ggtgcaggac
     3541 ctgaaccgct atcgcaagga agtcactgcg cgccttggcc aatcctcggt gcccagcgcg
     3601 gcgtaagcct ccgggatgga aaacctgaaa gccgcaagcc tgaccgcttg cggctttttc
     3661 attgcatgat cgattttgaa cgcaaactct gcgagcgcgc ggacctggcg cgccgcatcg
     3721 ccgacctccc gcgtccgctg gtgctgacca acgg
//
LOCUS       DATLGF010000427         2760 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_199916, whole genome shotgun sequence.
ACCESSION   DATLGF010000427 DATLGF010000000
VERSION     DATLGF010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199916"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..546
                     /locus_tag="VM406_13060"
     CDS             88..546
                     /locus_tag="VM406_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HVL76937.1"
                     /translation="MKFRSLAASTLAALLTAAASLAIAQDVQVTRSVARATVPQQQSA
                     GAYVTLENRGREADRLVGASTPVAKSVELHTMALDGNVMRMREVPAIDVPAGGRVEMK
                     PGEGFHLMLMGLQQQLKAGDTFGLTLRFERAGEVKTTATVESGRGGHHKH"
     gene            complement(615..1307)
                     /locus_tag="VM406_13065"
     CDS             complement(615..1307)
                     /locus_tag="VM406_13065"
                     /inference="COORDINATES: protein motif:HMM:NF023036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HVL76938.1"
                     /translation="MLRKTYSQLFAALLLLASAFAHASAPVIEVYKSQYCGCCEMWVE
                     HLRANGFTVKSNDVDDPSAWRAKLGIPAELGSCHSAKVGDYVIEGHVPAKEIKRLLAE
                     KPNAKGLAVPGMPLGSPGMEADRAEAYDVMLIAADGKHRVYQHYTAEDAARSTPVAAL
                     ADGEVRKVDMEKGRITIKHGPLANLGMPPMTMAFQADAGLIRTVQAGDKVRFSAEKSG
                     NGYRVTAVEVVR"
     gene            complement(1398..2411)
                     /locus_tag="VM406_13070"
     CDS             complement(1398..2411)
                     /locus_tag="VM406_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HVL76939.1"
                     /translation="MITIPHAALAEVTRGGQLESIHYGSVAVVDARGKLLYAAGDPDF
                     MTFTRSSIKPFQALPFLLDGGPAQFGFGPREIALQCASHSGEDMHVQVVEGMLKKAGC
                     DEHHLQCGCHLPMRYAQGEAPPAGMKFNQLHNNCSGKHAGFLAWCVQHHEALDTYLDP
                     AHPLQQAIRTALARVAGVMPGELVAGTDGCSAPNYALPLSRLAYTYARLAQGREDAEF
                     GPEMGTLFDAMTTHPELVSGTGRSDQAFMRTAPGDWVAKVGAEAVQVIGIRSAGIGLA
                     VKIADGNNRALYPAVIAALQQLGLLSHLEDTPLAAFARPPIRNARGLDVGEIRPLLEL
                     NRI"
     gene            complement(2408..>2760)
                     /locus_tag="VM406_13075"
     CDS             complement(2408..>2760)
                     /locus_tag="VM406_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HVL76940.1"
                     /translation="LARARSLGAALVVAVNTDASVRRLGKGEDRPVNPCDDRMALLAA
                     LESVDLVVPFDEDTALAVVEEARPDLYVKGGDYDMRTVPEALAAATQGGRALAIPFEY
                     QRSTTALLKKVRQS"
BASE COUNT          448 a          933 c          909 g          470 t
ORIGIN      
        1 tgtccaatac cgtcgctatc gtcgtctatg aggcctggcg ccagaatgac ttcgccggcg
       61 gcggctgatt cacccacagg agatcacatg aagtttcgtt cccttgccgc gagcacgctt
      121 gcagctctgc tcacggccgc cgcttccctc gcgattgctc aggatgtgca ggtgacgcgc
      181 tcggtcgcgc gcgcaacggt gccccagcag cagtcggccg gcgcctatgt cacgctggag
      241 aaccgcggcc gcgaggccga ccggctggtc ggggcctcca cgcccgtcgc aaagtcggtc
      301 gagctgcata cgatggcgct ggacggcaat gtgatgcgca tgcgcgaagt cccggcgatc
      361 gacgtgcccg ccggcgggcg cgtcgagatg aagcccgggg agggttttca cctgatgctg
      421 atggggctgc agcagcagtt gaaggcggga gacacgttcg ggctgacctt gcgcttcgaa
      481 cgcgcgggcg aggtgaagac gacggcgacg gtggaatccg ggcgcggcgg gcatcacaag
      541 cactgaacgg atggggcccg gccgaaggtg tttcggcggg cctgctaccc gctgcatccc
      601 tcaaatccca ggcttcagcg caccacctcg acggccgtca cgcgatagcc gttgccgctc
      661 ttctccgctg agaatcgcac cttgtcgccg gcctgcacgg tcctgatcag cccggcatcg
      721 gcctggaagg ccatcgtcat cggcggcatg ccgaggttgg cgagcgggcc gtgcttgatc
      781 gtgatgcggc ccttctccat gtcgaccttc ctcacctcgc catcggccag cgcggcgacc
      841 ggcgtcgacc tggcggcgtc ctccgccgtg tagtgctggt agacgcggtg cttgccgtcg
      901 gccgctatca gcatcacgtc atacgcttcg gcgcggtcgg cttccatgcc cggcgagccg
      961 agcggcatgc cgggaacggc cagccccttc gcgttcggct tctcggccag gaggcgcttg
     1021 atctccttcg ccggcacatg accttcgatc acatagtcgc caaccttggc ggaatggcat
     1081 gagccgagct cggcaggaat gccgagcttc gcgcgccagg cggacggatc gtcgacatcg
     1141 ttggacttga cggtgaagcc gttcgcgcgc aggtgctcga cccacatctc acagcagccg
     1201 caatactggc tcttgtagac ctcgatcacc ggcgcggacg catgcgcgaa tgccgacgcc
     1261 agcagcagca gcgcggcgaa caattgtgaa tatgtctttc tcaacatcag cggttttcct
     1321 tcttgcgatg aagcaccgac cagcccggtg cgcagactct gaccggaacc cgcgccgatc
     1381 gtgccccgaa ttcgtattca gatgcggttc agttcgagca acggccggat ttcgccgaca
     1441 tcgagcccgc gcgcgttcct gatcggcggc cgcgcgaacg ctgccagcgg cgtgtcttca
     1501 aggtgagaca gcaatccgag ctgctgcagc gcggcaatca cggccggata cagcgcgcga
     1561 ttgttaccgt cggcgatctt caccgccagc ccgataccgg ccgaacggat gccgatcacc
     1621 tgcaccgcct ccgcaccgac cttcgcaacc cagtcgcccg gcgcggtccg catgaaggcc
     1681 tggtcgctgc ggccggtgcc cgagaccagc tcggggtgcg tggtcatcgc atcgaacagc
     1741 gtgcccatct ccggtccgaa ctccgcatcc tcccggccct gcgccaggcg cgcatacgta
     1801 taggccaggc gcgagagcgg cagcgcatag ttcggcgccg agcagccgtc ggtgccggca
     1861 accagttcac ccggcatcac gcccgcaacc cgggccagcg ccgtgcgtat cgcctgctgc
     1921 agcggatgcg cggggtcgag gtaggtgtcg agcgcttcgt ggtgctgcac gcaccaggcc
     1981 aaaaagcccg cgtgcttgcc gctgcagttg ttgtgcagct ggttgaactt cattcccgcc
     2041 ggcggcgcct cgccctgcgc ataccgcatc ggcaggtggc agccgcactg caagtgatgc
     2101 tcgtcgcaac cggccttctt cagcatgcct tcgacgacct gcacatgcat gtcctcgccg
     2161 gagtggcttg cgcactgcag cgcaatttcc cgcggaccga agccgaactg cgcagggccg
     2221 ccgtcgagca ggaacggcag ggcctggaac ggcttgatgc tggaccgcgt gaaggtcatg
     2281 aaatccggat cgccggccgc atacagcaat ttgccgcggg catcgacgac ggcgacggat
     2341 ccgtaatgga tggattcgag ctgtccgccg cgcgtgactt cggcgagggc ggcgtgcggg
     2401 atggtgatca tgactggcgt accttcttca gcaaggcggt ggtggagcgc tggtattcaa
     2461 acggaatcgc gagcgcgcgg ccgccctgcg tggcagcggc gagcgcctcc ggaacggtgc
     2521 gcatgtcgta gtcgcctccc tttacgtaca ggtcgggtcg ggcctcttcg accacggcga
     2581 gcgcggtgtc ttcgtcgaac ggcacgacca ggtccaccga ttcgagtgca gccagcagcg
     2641 ccatgcggtc gtcgcaggga ttgacgggcc ggtcctcgcc cttgccgagc cgccgcaccg
     2701 aggcgtcggt attgaccgcg accacaagcg ccgctcccag cgagcgggca cgcgccaggt
//
LOCUS       DATLGF010000428         6088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_232983, whole genome shotgun sequence.
ACCESSION   DATLGF010000428 DATLGF010000000
VERSION     DATLGF010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232983"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VM406_13080"
     CDS             complement(<1..401)
                     /locus_tag="VM406_13080"
                     /inference="COORDINATES: protein motif:HMM:NF040011.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6151 family protein"
                     /protein_id="HVL76941.1"
                     /translation="MAHRIECKCGALGGTLEHGAEVNRAVCYCDDCQAFARFLGRDNE
                     VLDGAGGTEVIQTLQSRVRFERGHEHLACMRLSPRGMLRWYARCCNTPLGNTLPNHKL
                     AMFGLVHSCVQAEEGSLDRAFGPRKTIAFTK"
     gene            complement(703..1674)
                     /locus_tag="VM406_13085"
     CDS             complement(703..1674)
                     /locus_tag="VM406_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVL76942.1"
                     /translation="MKRLFAACVLALPVLAATAPAMAQSFPSKPIRWLVPYPAGGGSD
                     FLARTVGQQLSEQIKQPVIVENKPGGNTAIAAQETARSAPDGYTILNADNGTMVFNTA
                     LYSRLVYDAEKDLVPVTLVGRFPMILVVGPGSDAKDLKDFIEKAKAKPGSFTFGSAGA
                     GSPHQLAMELLKVNAGLDMLHVGYRGAAPALADLAGGQIQAMMVDLAAGAAFLKSNRV
                     RPLAVANATRLPQLPDVPTFAELGFKDVEAAALVGVVARAGTPPEVVNTLYKQLSTAI
                     NTPSVRERLVEFGIEPVANTPQQYAQLLTQERQRWHKLIRDQKITLD"
     gene            1851..2657
                     /locus_tag="VM406_13090"
     CDS             1851..2657
                     /locus_tag="VM406_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HVL76943.1"
                     /translation="MEQTTEQRPVERRQRVQSAEMGMSVLKALARLGGSASLTAVAAE
                     IGESTAKVHRYMASLTQEGLVAQDPATQHYYLGREAIQIGLAALRQCDPIRLGEAALV
                     RLREQLEVTCFIAVMGNKGPTILRFEEPGLPVTINVRAGSVLPLLWSATGRAFLGYLD
                     EAHVRRLADEELARANKEQRASLHGRNPIEQLRKEVREQGCAVINDILLRGISAVAAP
                     IFDYTGRVSAVLTALGASGGFDTDPQGRIASMVRHEAADISAALGARAKP"
     gene            2783..5104
                     /locus_tag="VM406_13095"
     CDS             2783..5104
                     /locus_tag="VM406_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450/oxidoreductase"
                     /protein_id="HVL76944.1"
                     /translation="MKTSTSSCPIDHQALRTGGCPVSARAAQFDPFEDAYQQDPPEYV
                     RWSREEEPVFYSPKLGYWVITRYDDIKAVFRDNITFSPSIALEKITPTGEEANAVLAS
                     YNYAMNRTLVNEDEPAHMARRRVLMEPFTPEALRHHEPMVRRLAREYVNRFIDDGRAD
                     LVDQMLWEVPLTVALHFLGVPEEDMDLLRQYSIAHTVNTWGRPKPEEQVKVAHAVGNF
                     WQLAGKILEKMRKDPDGPGWMKYGIRKQAEYPDVVTDSYLHSMMMAGIVAAHETTANA
                     TANAIKLLLQHPSAWREICEDPSLIPNAVEECLRHNGSVPAWRRLATKDVKIGGIDIP
                     AGSKLLIVMASANHDQRHFEDADALDIRRDNASDHLTFGYGSHQCMGKNLARMEMQIF
                     LEELSKRLPHMRLAEQTFTYLPNTSFRGPEHLWVEWDPRENPERKNPALLDSSSPVRI
                     GEASAHVVSRTVVVDSVTRLTDEIVRLRLKSPDGRALPRWMPGAHIDVECGNPDISRQ
                     YSLCGDPDDPHTLEIAVLRETESRGGSSWIHDTVKPGDRLRIRGPRNHFRFDEGVRRA
                     IFIAGGIGITPIIAMARRAKALGIDYELHYSGRSRSSMACLDELSALHGERLHVYARD
                     EGKRADYAALLATPAEGTRIYACGPVRLLEALEQCCAAWPEDALRVEHFHTTRGSLDP
                     SKEKAFEVELKDSGLVVQVAADQTLLAALRGANIDVQSDCGEGLCGSCEVRVLAGEVD
                     HRDVVLTRSEREANNKMMACCSRAACGRLVLEL"
     gene            complement(5598..>6088)
                     /locus_tag="VM406_13100"
     CDS             complement(5598..>6088)
                     /locus_tag="VM406_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HVL76945.1"
                     /translation="YPLLVDAENWMSHPGAGEAILNIFRHPKTPQNFVILSGDVHYSF
                     VYDVALRGRGHGREPDIWQICNSGLRNEFPPRLLDVLDRANRWLYSPRSPLNWLTRRR
                     HMRVTPRKPEGIPSGRRLLNGSGIGLVELDPNGVPWRIRALLADERWVSFTPREAESR
                     WD"
BASE COUNT         1145 a         1977 c         1952 g         1014 t
ORIGIN      
        1 aacttcgtga aggcgatcgt cttgcgcggt ccgaatgcgc gatcgagcga accttcctcc
       61 gcctgcacgc agctgtgcac caggccgaac atcgcgagct tgtgattcgg cagggtgttg
      121 ccgagcggcg tattgcagca gcgggcatac cagcgcagca tcccgcgcgg cgagaggcgc
      181 atgcaagcca ggtgctcgtg tccgcgctcg aagcgcacgc gcgattgcaa ggtctggatg
      241 acctcggtgc cgccagcgcc gtcgagcacc tcgttgtctc gaccgagaaa gcgcgcgaag
      301 gcctggcaat cgtcacagta gcagaccgca cggttgactt cggcgccgtg ttccagcgtt
      361 ccgccaagcg cgccgcactt gcattcgatc cgatgtgcca tggcatggct ccatgaagat
      421 gacgtgtcat cttacgccac gctgcgcttc atgcgggcgc gcccgtcacg cttcggcccg
      481 tgtccccgcc accaaagcgg tctcgtggcg gagcgcaaac caccaaattc gaaggatcag
      541 gatgacccga cgtggcgagc gcaccagaag atggctgatg cccggacggc ctgactcgtg
      601 gagtgaaggc catgcaacag aaacggggtc aggtcctgcc gtttgtccca ccaaggacaa
      661 ccggcaaaac ctgaccccgc aattccggac aggaacggga gtttaatcca gcgtaatctt
      721 ctgatcgcgg atcagcttgt gccagcgctg gcgctcctgg gtcagcagct gcgcgtattg
      781 ctgcggcgtg ttggctaccg gctcgatgcc gaattcgacc aggcgttcgc gaaccgacgg
      841 ggtgttgatt gcagtcgaga gctgcttgta cagcgtattc acgacctcag gcggggtgcc
      901 ggcccgtgcc accacgccga ccagcgcggc ggcttccaca tccttgaacc cgagttcggc
      961 aaaggtcggc acatcgggca gttgcggcag gcgggtcgca ttggcgaccg cgagcgggcg
     1021 cacgcggttg ctcttgagga aggctgcgcc tgctgcaagg tcgaccatca tggcctggat
     1081 ctggccgccg gccaggtcgg caagggccgg cgccgcacca cggtagccta cgtgcagcat
     1141 gtcgagcccg gcgttcacct tcaacagttc cattgccagt tggtgcgggc tgcctgcacc
     1201 ggccgagccg aaggtgaagc tgcccggctt ggccttggcc ttttcgatga agtccttcaa
     1261 atcctttgcg tcggacccag ggccgacgac gagaatcatc ggaaagcggc cgaccagcgt
     1321 caccggtacc agatcctttt cggcgtcgta caccagccgc gaatagagcg cggtgttgaa
     1381 caccatggtg ccgttgtcgg cgttgaggat ggtgtagccg tccggcgccg agcgcgcggt
     1441 ttcctgcgcg gcgatggcgg tattgccgcc gggcttgttc tccacgatga ccggctgctt
     1501 gatctgctcc gacaattgct gtcccacggt gcgggccagg aagtcggaac cgccgccggc
     1561 cgggtagggc accagccagc ggatcggctt ggacgggaag gattgcgcca ttgccggtgc
     1621 ggtagccgcc aaaaccggca gagccaatac gcatgcggcg aacagtcttt tcatgaattt
     1681 tctcctcgtt gaaatggatg ctgcgctatt tttgcgtaac gctactacgc aaagcgtaaa
     1741 attacaagca ggcctcggct gacccccgtc acgatttgca gcggggctga cattgacaca
     1801 ggaattccac gccaccacag taacggcatt tcagacaggc agcgaaagca atggaacaga
     1861 cgacagagca gcggccggtg gagcggcgcc agcgggtgca atcggccgag atgggcatga
     1921 gcgtgctcaa ggcgctggcc cgcctcggcg gctccgccag cctcaccgcc gtggcggcgg
     1981 aaatcggcga gagcaccgcc aaggtgcacc gctacatggc cagcctgacg caggaaggtc
     2041 tggtcgcaca ggatcccgcc acccagcact actacctcgg gcgcgaagcc atccagatcg
     2101 gcctggccgc gctgcgtcag tgcgatccga tacgcctggg cgaggccgca ctggtacgtc
     2161 tgcgcgagca actggaagtg acctgcttta tcgcggtgat gggcaacaag ggcccgacca
     2221 tcctgcgttt cgaggaaccc ggcctgccgg tcaccatcaa tgtacgcgcg ggctcggtgc
     2281 tgccgctgct gtggtcggcc accgggcgcg catttctcgg ctatctcgac gaggcgcacg
     2341 tgcgccggct ggccgatgaa gaactcgcgc gcgcgaacaa ggaacagcgc gcctcgctgc
     2401 acggcaggaa tccgatcgag caactgcgca aggaagtgcg cgaacagggc tgcgccgtca
     2461 tcaacgacat tctcctgcgc ggcatcagcg cggttgccgc ccccatcttc gactacaccg
     2521 ggcgggtcag cgcggtgctg accgcgcttg gcgcctcggg cggcttcgat accgatccgc
     2581 aaggcaggat cgccagcatg gtccggcacg aggcggccga catcagcgcc gcccttggcg
     2641 cgcgcgcaaa accgtaaatc cgatgattcg caagccccgt cttcgcacgc ctttactatt
     2701 acgcataacg tagtaagatt acgcatagcg aaggcccgcc ggcctgcttt gccgatatcc
     2761 gcccctcacg gaagcgcaca ccatgaaaac atcgacctca tcctgcccga tcgaccacca
     2821 ggcactcagg accggcggct gcccggtcag cgcgcgcgcg gcgcaattcg atccgtttga
     2881 ggacgcctac cagcaggatc cgcccgaata cgtgcgctgg tcgcgcgagg aagagccggt
     2941 gttctacagc ccgaagctcg gctactgggt gatcacccgc tacgacgaca tcaaggccgt
     3001 gttccgcgac aacatcacct tcagtccgtc gatcgcgctg gaaaagatca cgcccacggg
     3061 cgaggaagcc aatgcggtgc tggcttcata caactacgcg atgaaccgca cgctggtgaa
     3121 cgaggacgag ccggcccaca tggcgcggcg gcgcgtgctg atggagccgt tcacccccga
     3181 agccctgcgc catcacgagc cgatggtgcg ccgcctggcg cgcgaatacg tcaaccgctt
     3241 catcgacgac ggccgcgccg acctggtcga ccagatgctg tgggaagtcc cgctgaccgt
     3301 ggcgctgcac ttccttgggg tgcctgaaga agacatggac ctgctgcgcc agtactcgat
     3361 tgcccacacc gtcaacacct ggggtcggcc caagcccgaa gagcaggtga aggtcgcaca
     3421 tgcggtcggc aacttctggc agctggcggg caagatcctc gaaaagatgc gcaaggatcc
     3481 ggatggtccg ggctggatga agtacggcat ccgcaagcag gcggaatatc ctgatgtggt
     3541 caccgactcc tacctgcact cgatgatgat ggccggcatc gtcgccgccc atgaaaccac
     3601 ggccaacgcc actgccaatg cgatcaagct gctgctgcag cacccgagcg cctggcgcga
     3661 aatctgcgaa gacccgagcc tgatccccaa tgcggtggaa gagtgcctgc gccacaacgg
     3721 ctcggtgccg gcctggcggc gcctggcgac gaaggatgtg aagataggcg gcatcgacat
     3781 cccggccggc tcgaagctcc tgatcgtgat ggcctcggcc aaccacgacc agcgccactt
     3841 cgaggatgcg gacgcgctcg acatccgccg ggacaacgcg agcgatcacc tgaccttcgg
     3901 ctatggctcg caccagtgca tgggcaagaa cctggcccgc atggagatgc agatcttcct
     3961 cgaggagctg agcaagcgcc tgccgcacat gcgcctggcc gaacagacct tcacctatct
     4021 gccgaacacg tccttccgcg gccccgagca cctgtgggtc gagtgggatc cgcgcgagaa
     4081 tcccgagcgc aagaacccgg cactgctgga cagcagctca ccggtacgca tcggcgaagc
     4141 gtcggcccac gtggtcagcc gcaccgtcgt tgtcgacagc gtcacgcgcc tgaccgacga
     4201 gatcgtgcgg ctgcgcctga aatcgccgga cgggcgcgcg ctgccgcgct ggatgccggg
     4261 cgcgcacatc gacgtggagt gcggcaatcc ggacatctcg cgccaatact cgctgtgcgg
     4321 cgacccggac gatccccaca cactcgagat cgccgtgctg cgcgaaactg agagccgtgg
     4381 cggctccagc tggatacacg acacggtcaa gcccggcgac cggctcagga tacgcggccc
     4441 gcgcaaccac ttccgtttcg acgaaggcgt gcgccgtgcg atcttcattg ccggcggcat
     4501 cggcatcaca cccatcattg cgatggcgcg acgcgcgaag gcgctgggca tcgactacga
     4561 actgcactac agcggacgct cgcgcagcag catggcctgc ctggacgagc tgtcggcact
     4621 gcatggcgag cgcttgcatg tctacgcgcg cgacgaaggc aagcgagcgg actacgccgc
     4681 gctgcttgcc accccggccg aaggcacccg catctacgca tgcggcccgg tgcgcctcct
     4741 ggaggcgctg gaacagtgtt gcgcagcctg gcccgaagac gcgctgcgcg tcgagcactt
     4801 ccatacgacg cgcggcagcc tcgaccccag caaggaaaaa gccttcgagg tggagctgaa
     4861 ggattccggc ctcgtcgttc aggtagcggc cgaccagacg ctgctggcgg ccctgcgcgg
     4921 cgccaacatc gacgtgcaga gcgactgcgg cgaaggcctg tgcggatcct gcgaggtgcg
     4981 cgtgctggcg ggagaggtgg atcaccgcga cgttgtactc acccgcagcg agcgcgaggc
     5041 gaataacaag atgatggcgt gctgctcgcg cgcggcttgc gggaggttgg tgctggagtt
     5101 gtagagcgga ccgtaagccg gggcaagtct gctgtcttgc ccttgcttgg aattcggcgg
     5161 gatagatcga tgcaaaagga aagacctgac ccgtttgctc tccctatgcg ccctttgctc
     5221 gtttttctca gacgatctgc ttcgtcatct cattgatgac ggtgcgcaga acggctctga
     5281 tgcagcgcga ttcggttttg gcgcgattag cggtactcgc cgggtgcctg cggggattgc
     5341 gacaaagcgc tcagcagggt cccgcgccaa gaactcggtc gactactgcg cccagttcgc
     5401 gcgctccagc acatcgcccg ccatgtccaa gagcatcggg tgggagcgga tttcagacca
     5461 ctccgtgcgg cacgcgcggg gttgaactcg atacagcgct ggcagttcag cggatatctg
     5521 cgcctattat ctgcgctgcg ttgatgcgca tggacagcga acaaggcatc catgcctggc
     5581 acgggaactg gtgccgatca gtcccagcgc gattcggcct cgcgcggcgt gaagcttacc
     5641 cagcgttcgt cggccagcag cgcgcgtatt cgccacggta cgccattcgg gtcaagctcc
     5701 accaggccga tcccgctgcc gttcagcaag cgtcgtcccg atggtatgcc ctcgggcttg
     5761 cgcggcgtga cgcgcatatg cctgcgccgg gtcagccagt tcagcggcga gcgcggagag
     5821 tacagccagc ggttggcccg atccagcacg tcgagaaggc gcggcgggaa ttcattgcgc
     5881 aggccgctgt tgcagatctg ccagatgtcc ggctcgcgcc cgtgtccgcg tccacgcagc
     5941 gcgacgtcgt agacgaatga atagtgcaca tcgcccgaca atatgacgaa gttctgcggc
     6001 gttttcggat gccggaagat attcaggatc gcctcgccgg cacccggatg cgacatccag
     6061 ttttctgcat cgaccagcag cggatacc
//
LOCUS       DATLGF010000429         3996 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_183923, whole genome shotgun sequence.
ACCESSION   DATLGF010000429 DATLGF010000000
VERSION     DATLGF010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183923"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1287
                     /gene="senA"
                     /locus_tag="VM406_13105"
     CDS             103..1287
                     /gene="senA"
                     /locus_tag="VM406_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1903257 - selenoneine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine synthase SenA"
                     /protein_id="HVL76946.1"
                     /translation="MNTSFRTATPDELARALRDARERTLLLFEGFVQAGYQNPARSSL
                     AAEASSPLWELGHIAWFAEWYVLRGAQSSEPAAAQRASMLSRGDDWFDPNTLPRQGAP
                     LDLPSVGALKTYCREVQDRVIDRLSRAGKEDKDLYPYRLALAYEDMWGERWMQAYQLL
                     DLALPPELAERKALPWAQGEIRFAGGTIRLGSEPGSGFVFDNEKWAHECYVAPFTMDS
                     TLVSNAQFAEFVGDGGYQESRYWSTAGRAWLVSHGRYAPRDWRRDGRHWRAQRFGRMV
                     ALRAHDPVRHLNLFEAQAYCMWAGRRLPTEAEWEYAALTAHPALHWGDLWEWTCSPFE
                     PYPGFSPDAWRGYSEPYFGKHQVLRGASFATSVRMRSPKLRAFRWPEDDTLFAGLRTC
                     AR"
     gene            complement(1298..2071)
                     /locus_tag="VM406_13110"
     CDS             complement(1298..2071)
                     /locus_tag="VM406_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HVL76947.1"
                     /translation="MFGFGLGGILILVLALLASAVRVLREYERGVVFQLGRFWAVKGP
                     GLIIVIPVLQQMVRVDLRTIVLDVPTQDVISRDNVSVKVNAVLYFRVVDPEKAIIQVE
                     NFFEATSQLAQTTLRAILGKHELDDMLSEREQLNFDIQQVLDAQTDGWGIKVINVEIK
                     HVDLDESMVRAIARQAEAERERRAKVIHAEGELQASEKLQQAAQVLAVQPGAMQLRYL
                     QTLSTIAGDKNSTIVFPVPMELVAQISSLAQSLAEKENT"
     gene            complement(2074..3516)
                     /locus_tag="VM406_13115"
     CDS             complement(2074..3516)
                     /locus_tag="VM406_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HVL76948.1"
                     /translation="MSTASPDRANRTEKAIIRSRPALFLLYLLLVLAWVHAPARAAPA
                     PVALLTVSGAIGPASADYISRGIARAEREGAQLVVLQMDTPGGLDLSMRTIIKAILAA
                     QVPVATFVGPQGARAASAGTYILYASHVAAMAPGTNLGAATPVAVGGGEQPERPQREP
                     QPEKDAPAPRTKGEPAGEPTTSPSTRKQVNDAAAYIRGLAQMRGRNMEWAERAVREAV
                     SLSAEEAVKQNVVDLVARDVNDLLSQLDGRTINVAGQDRTLETANAQVDSYEPDWRVR
                     FLGVITDPSIALLLMTIGMYGLILEFSNPGVGVPGVLGAISLLLALYALQLLPVNYAG
                     VALILLGLGFMIAEAFVPSFGILGLGGIAAFVTGAVILIDTEVPGYGIPLALILTIAI
                     FCALLVILTVTVMLKTRRRPTVTGQGGLIGSIAEVIDPTPDHGWVTLHGETWRAHSEA
                     PLRREQKVRVTGRDGLVLEVAPIDQDKKGE"
BASE COUNT          637 a         1336 c         1297 g          726 t
ORIGIN      
        1 catttccgac acagggctgc acgctgcgct gcgcctcctt gccgtagtgc atgtgccgac
       61 cgctggcctc gtcgttcggc agcggcgata atgcgctctt cgatgaacac ttcctttcgt
      121 accgccaccc ccgacgaact cgcccgcgcc ctgcgcgacg cgcgcgagcg cacgctgctg
      181 ctgttcgagg gtttcgtgca ggcgggttac cagaatccgg cccgctcctc gctcgccgcg
      241 gaggcgagct cgccgttgtg ggaactcggg catatcgcct ggtttgcgga atggtatgtg
      301 ctgcgcggcg cccaatcgag cgagccggcg gccgcccagc gcgcgtccat gctgtcgcgc
      361 ggcgacgact ggttcgaccc caacacgctg ccccggcagg gggcaccgct cgatctgccc
      421 agcgtcggcg cattgaaaac ttattgccgg gaggtgcagg atcgcgtgat cgaccggctc
      481 tcgcgcgcag gcaaggaaga caaggatctc tacccctatc gcctggccct ggcctacgaa
      541 gacatgtggg gcgagcgctg gatgcaggcc tatcagctgc tcgacctggc gttgccgccg
      601 gagctggctg aacggaaggc gcttccatgg gcgcaggggg agatccgttt tgcgggcggc
      661 accatccgct tgggcagcga gcccggcagc ggcttcgtat tcgacaacga gaaatgggcg
      721 cacgagtgct acgttgctcc tttcacgatg gattcgaccc tggtgtccaa tgcccagttt
      781 gcggaatttg taggcgacgg cggctaccag gagtcgcggt actggagcac cgccgggcgc
      841 gcgtggctgg tctcgcacgg ccgctacgcc ccgcgcgact ggcgtcgcga cggcaggcac
      901 tggcgcgccc agcgtttcgg ccgcatggtc gcgctgcgcg cgcacgaccc ggtacgtcat
      961 ctgaacctgt tcgaggccca ggcctattgc atgtgggccg gccgccgcct gccgaccgag
     1021 gcggagtggg aatacgctgc gctgaccgca caccctgcgc tgcactgggg tgacctgtgg
     1081 gagtggacct gctcgccgtt cgagccttac cccggctttt cgccggatgc atggcgcggc
     1141 tattccgaac cctacttcgg caagcaccag gtattgcgcg gggcgtcctt cgcgacgtcg
     1201 gtacggatgc gctcgccgaa gttgcgcgcg ttcaggtggc cggaggacga tacgctgttc
     1261 gcagggctgc gcacctgcgc gcgctgaccc tcggcgctta ggtattttcc ttttccgcca
     1321 gcgactgcgc gagcgaggag atctgcgcta cgagttccat cggaaccgga aacacgatcg
     1381 tcgaattctt gtcgcccgca atcgtgctca gcgtctgcag atagcgcagc tgcatcgcac
     1441 ccggctgcac cgccagcacc tgggccgcct gctgcagctt ttccgacgcc tgcaattcgc
     1501 cttcggcgtg aatcaccttc gcgcgccgtt cgcgctcggc ctcggcctga cgggcaatcg
     1561 cccgcaccat cgactcgtcc aggtccacgt gcttgatctc gacgttgatg accttgatcc
     1621 cccagccgtc ggtctgcgcg tcgagcacct gctggatatc gaagttcagt tgctcgcgct
     1681 cggacagcat gtcatcgagt tcatgcttgc cgaggatcgc gcgcaaggta gtctgcgcga
     1741 gctggctggt cgcctcgaag aaattttcga cctggatgat cgccttttcc ggatcgacca
     1801 cgcggaagta cagcacggcg ttcaccttca ccgagacgtt gtcgcgcgag atcacgtcct
     1861 gggtcggcac gtcgagcact atggttcgca ggtccacgcg caccatctgc tgcagcaccg
     1921 ggatcacgat gatcaggccg ggccctttca ccgcccagaa gcggccgagc tggaacacca
     1981 cgccgcgttc gtattcccgc aggactcgaa cggcggaagc gagcagcgcc aggacgagga
     2041 tgaggatgcc gcccaatccg aaaccgaaca tgcttattct ccttttttgt cttggtcgat
     2101 cggtgccact tccagcacca gcccgtcgcg tccggtcacc cggaccttct gttcgcgccg
     2161 cagcggtgct tcgctgtgcg ctcgccaggt ttccccgtgc agcgtgaccc agccgtggtc
     2221 gggggtcggg tcgatcactt cggcgatgct gccgatcaag ccgccctggc cggtgacggt
     2281 tggacgcctg cgggtcttga gcatgactgt caccgtcagg atcaccagca gcgcgcagaa
     2341 gatggcgatc gtgaggatca gcgcgagcgg aatcccgtag cccggcacct cggtgtcgat
     2401 cagaatcacc gcgcctgtca cgaaagccgc gatgccgccc agcccgagta tcccgaaact
     2461 cggaacgaag gcctcagcga tcatgaagcc cagcccaagc aggatcagcg ccacccccgc
     2521 gtagttcacc ggcagcagct gcagcgcgta aagcgcgagc agcagcgaga tcgcgccgag
     2581 cacacccggc acgccgacgc ccggattgct gaattcgaga atcagcccgt acatgccgat
     2641 ggtcatcaga agcagggcga tgctcggatc ggttatgacg ccaagaaagc gcacgcgcca
     2701 gtccggctcg tagctgtcga cctgcgcgtt cgcggtctcc agcgtccggt cctgtccggc
     2761 gacgttgatc gtcctgccgt cgagttggct cagcagatcg ttcacatcgc gcgcaaccag
     2821 gtccaccacg ttctgcttga cggcctcctc ggcggacagg ctgacggctt cgcgcaccgc
     2881 ccgctcggcc cattccatgt tgcgcccccg catctgcgca aggccgcgga tgtaggcggc
     2941 cgcgtcgttg acctgcttgc gtgtgctcgg agaagtggtg ggttcgccgg ccggctcgcc
     3001 cttggtgcgc ggcgccggcg catccttttc cggctggggt tcgcgctgcg ggcgctccgg
     3061 ctgctctccg ccgccgaccg cgaccggcgt ggcggccccg agattggtgc cgggcgccat
     3121 cgcggcgaca tggcttgcat aaaggatata agtgccggcg cttgcggcgc gtgcgccctg
     3181 cgggccgacg aaggtggcga ccggaacctg ggcggccagg atggccttga tgatggtgcg
     3241 catcgagaga tccagtccgc ccggcgtgtc catctgcagc accacgagct gggcgccttc
     3301 gcgttcggcg cgcgcgatgc cgcggctgat gtagtccgcg ctcgccggac cgatcgcgcc
     3361 cgagaccgtg agcagcgcca ccggtgcagg cgcggcccgg gcaggcgcgt gcacccatgc
     3421 cagcacgagc agcagataca gcaggaagag ggcggggcgg gaacggatga tggctttctc
     3481 cgtgcggttc gcgcgatcag gcgacgcagt actcatgata gggctgatcc gttagacaac
     3541 aagaactctg gacattgcat caggcgggct cacgcttgcg catgatcagc ttcgccctgc
     3601 tcgtgcgccg atccgtacgt atttgcacct attcgggtcg aaatgctgcc aaccggtata
     3661 ggcgcgccag tatcactcgg atacaatggc gggcgcaaaa ttggcggtga cgaaacatcc
     3721 ggcgcgcttc gatagtgtga aagatgcaag ggcttgttcc ctgccaaccc atccgccgtt
     3781 caaccagcgg cccaggcggc tgcaaggcgg ccgacggccg cccgccgttc cgttcaacga
     3841 taactatatg gagtgcgctt gttttccacg cttgccctga tagtcctgct gccattcgcc
     3901 gcatgctggt tgccggtggt ggccgaacgc agcggccgca acgcctcggc ctggagcgcc
     3961 gggctggtcc tcgcggcggc attgctgctg ctcttc
//
LOCUS       DATLGF010000430         3626 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_141841, whole genome shotgun sequence.
ACCESSION   DATLGF010000430 DATLGF010000000
VERSION     DATLGF010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141841"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="VM406_13120"
     CDS             complement(<1..1057)
                     /locus_tag="VM406_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HVL76949.1"
                     /translation="MSEFLIANMAPLMFGALIVFLLSGFPIAFSLAANGLFFGFLGIE
                     LGLLQPALLQALPNRLFGIMGNDTLLAIPFFTLMGLILERSGMAEDLLDTIGQLFGTL
                     RGGVAYAVVFVGALLAATTGVVAASVISMGLISLPVMLRYGYDKRIATGVIAASGTLA
                     QIVPPSLVLIVMADQLGRSVGDMYRAALFPALLLVALYAGYILSVSIFKPKAVPALPP
                     EARNLREPDGSSGVISLLVLLAVTVATYYGFTAWYGETNQTPVFAGGAAVAVALVAAT
                     VNRFFKIGLLSRMAEKVVFVMIPPLALIFLVLGTIFLGIATPTEGGGMGAAGAIILAA
                     LNRRLTFSLLKQAMDSTS"
     gene            complement(1077..1655)
                     /locus_tag="VM406_13125"
     CDS             complement(1077..1655)
                     /locus_tag="VM406_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HVL76950.1"
                     /translation="MSFFLALARIIDTINEKIGLTISWALLVAVIICAGNAIMRYSFS
                     MSSNAWLEIQWYLYSAVFLIAASYTLRRNEHVRIDVVAGRFSKRTQIWIDAFGFLFFL
                     LPVTILTLYYSIPYTILSIEGREMSSNAGGLIVWPAKILIPIGFTLLALQGISEFIKR
                     VGYLMGRVDASEFEKQGPSPVEQLAGAHETKE"
     gene            complement(1921..3315)
                     /gene="argH"
                     /locus_tag="VM406_13130"
     CDS             complement(1921..3315)
                     /gene="argH"
                     /locus_tag="VM406_13130"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HVL76951.1"
                     /translation="MTAQLSKKSEAWSARFSEPVSDLVKRYTASVFFDKRLADADIAG
                     SLAHAEMLAHQGIISAQDYADIQRGMAQIQSEIAEGRFEWLLDLEDVHLNIEKRLTEL
                     VGDAGKRLHTGRSRNDQVATDIRLYLRGAIDDILGLLRELRIALLDLAERHAETILPG
                     FTHLQVAQPITFGHHMLAYVEMFSRDAERMADCRKRVNRLPLGAAALAGTTFPIDRER
                     VARTLGFDDVCHNSLDAVSDRDFAIEFCAAAALVMTHVSRMSEELIIWISPRVGFIDI
                     ADRFCTGSSIMPQKKNPDVPELARGKTGRVNGHLIGLLTLMKGQPLAYNKDNQEDKEP
                     LFDTVDTVIDTLRIFADMSRGITVKPDAMRAAALQGFATATDLADYLVKKGLPFRDAH
                     EAVAHAVRACEQRGCDLADLSLDELRGFSDLIEADVYTVLTPEGSVASRNHVGGTAPQ
                     QVRAAIARQRALLA"
     gene            3377..>3626
                     /locus_tag="VM406_13135"
     CDS             3377..>3626
                     /locus_tag="VM406_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76952.1"
                     /translation="MPARTPSAALVVPDCCNIGVVFRILLVVNAAVFCAALLQSPSVG
                     AGVQAFVDASMVVELACLWSLFAVCILRRLLLRARDGYA"
BASE COUNT          695 a         1073 c         1194 g          664 t
ORIGIN      
        1 ggctggtcga gtccattgcc tgcttcagca ggctgaacgt gaggcggcga ttgagcgcgg
       61 ccaggatgat cgcgcccgca gcccccatgc cgccgccttc ggtcggcgtg gcgatgccga
      121 ggaagatcgt gcccaggacc aggaagatca gcgcgagcgg cgggatcatc acgaacacga
      181 ccttctcggc catgcgggac aacaggccga tcttgaagaa gcggttgacc gtcgccgcga
      241 cgagtgccac ggccaccgcg gcgccgccgg caaacaccgg ggtctggttg gtctcgccat
      301 accacgctgt gaagccgtag taggtcgcca cggtgacggc aagcagcacc agcagggaaa
      361 tcacgccgct gcttccatcc ggctcgcgca gattgcgagc ttccggcggc agcgccggaa
      421 cggccttcgg cttgaagatc gacacggaga ggatgtagcc ggcgtacagc gctaccagca
      481 gcagggcagg gaacagcgct gcgcggtaca tgtcgccgac cgagcggccg agctggtcag
      541 ccatcacgat cagcaccagc gacggcggca cgatctgggc gagcgtgccg gatgcggcga
      601 tgacgcctgt cgcgattcgc ttgtcgtagc catagcgcag catgaccggc agcgagatca
      661 gtcccatcga aatcaccgag gcggccacaa ccccggtggt cgcggccagc agggcgccga
      721 caaacaccac cgcgtaggcc accccgccgc gcagcgtgcc gaacagctgg ccgatagtgt
      781 cgagcaggtc ttctgccatg ccggagcgct cgaggatcag gcccatcagc gtgaagaaag
      841 gaatcgccag cagggtgtcg ttgcccatga tgccgaacag gcgattcggc agcgcctgca
      901 ggagggcagg ctgcagcaat ccgagttcga ttccgagaaa accgaaaaac aggccgttgg
      961 cggcgaggga aaacgcaatc ggaaagcccg atagcaggaa cacgatcagt gcgccgaaca
     1021 tgagcggggc catgttcgcg ataaggaact ccgacattct cttctccgat tcttgattat
     1081 tctttggtct cgtgagcgcc ggccagctgc tcgaccgggg acgggccttg cttctcgaat
     1141 tcgctggcgt cgaccctgcc catcaggtag ccgacacgct tgatgaattc ggagatgccc
     1201 tgcagcgcga gaagggtgaa gccgatcggg atcaggatct tggcgggcca gacgatcagg
     1261 ccgcctgcat tgctcgacat ttcccggcct tcgatggaga gaatggtgta ggggatcgaa
     1321 taatagagag tgaggatcgt gaccggcagc aggaagaaca ggaagccgaa ggcgtcgatc
     1381 cagatttgcg tgcgcttcga aaacctgccc gcgacgacgt cgatgcgcac gtgctcgttg
     1441 cgacgcaacg tataggaagc cgcaataaga aacaccgccg aatacaagta ccactggatt
     1501 tccagccatg cgttcgagct catgctgaat gagtaacgca tgatggcatt gccggcgcag
     1561 atgataactg ccacgaggag ggcccagctg atggtcaggc ctatcttttc attgattgtg
     1621 tcaatgattc gcgcgagcgc gaggaaaaaa gacatgatga ctgtctcctg attgtgatga
     1681 attcttatga gccggtcgcc tgctgttctt gtatgcgaaa gcggctcttt atgtaggggc
     1741 aagactcgcg gcagtatatg tcatttcatt gttctcgcga acctttgcgg gaacgtcccc
     1801 ggcggtcgga accgcaggcc ggtaaaacta cgtatttcct tacggacgca ggcgaaaatt
     1861 ttcatgcatc gctacgtata aacacgtagt tcgggccggc gggtcgacgc tgtccgggca
     1921 ctaggcgagc agtgcgcgct ggcgggcgat cgccgcgcgt acctgctgcg gcgcggtgcc
     1981 gccgacatgg ttgcgcgaag cgaccgagcc ttccggcgtc agcacggtat agacatccgc
     2041 ttcgatcagg tccgagaagc cgcgcagctc gtccaggctc aagtctgcca ggtcgcagcc
     2101 ccgctgctcg caggcacgta ccgcatgagc aaccgcctcg tgggcgtcgc ggaaaggcag
     2161 gcccttcttc accagatagt cggccaggtc ggtggcggtg gcaaaacctt gcagcgcggc
     2221 agcgcgcatc gcatccggct tgacggtgat gccgcgcgac atgtcggcga agatgcgcag
     2281 cgtatcgatc acggtgtcca ccgtgtcgaa cagcggttcc ttgtcttcct ggttgtcctt
     2341 gttgtaggca agcggctggc ccttcatcag tgtcagcagg ccgatcaggt gaccgtttac
     2401 ccgcccggtc ttgccgcgcg ccagttcggg tacatcagga ttctttttct gcggcatgat
     2461 cgacgaaccg gtgcagaagc gatccgcgat gtcgatgaag ccgacgcgcg ggctgatcca
     2521 gatgatcaac tcttccgaca tccgggagac gtgcgtcatc accagcgcgg cggcggcgca
     2581 gaattcgatt gcaaaatccc ggtccgagac ggcgtcgagc gagttgtggc agacgtcgtc
     2641 gaagcccagc gtcctggcca cccgttcgcg atcgatcggg aaggtggtgc cggccagcgc
     2701 cgcggcgccg agaggaaggc ggttgacgcg cttgcggcag tcggccatgc gttcagcgtc
     2761 acgtgaaaac atttcaacat aggccagcat gtggtggccg aaggtgatcg gctgcgctac
     2821 ctgcaggtgt gtgaagccgg gaagaatcgt ttcggcgtgc cgttcggcca ggtcgagcag
     2881 ggcgatgcgc agctcgcgca gcaggccgag gatatcgtcg atggcgccgc gcagatagag
     2941 gcggatgtcg gtggcgacct ggtcgttgcg agaacggccg gtgtgcaggc gcttgccggc
     3001 gtcaccgacc agctcggtca gccgtttttc aatgttcagg tgcacgtctt ccaggtcgag
     3061 cagccattcg aacctgcctt cggcgatttc gctctggatc tgcgccatgc cgcgctggat
     3121 gtctgcatag tcctgggcgc tgatgatgcc ctggtgcgcc agcatttccg catgggcgag
     3181 cgagccggcg atgtcggcgt ccgcaaggcg cttgtcgaag aagacggagg cggtgtagcg
     3241 tttgacgagg tcggagacgg gttcggaaaa gcgggccgac catgcctcgc ttttcttgga
     3301 aagttgtgct gtcatatcga gcatcctgag gaagaccgaa ttatacctac cgacctgccg
     3361 aacacccaac ctcccgatgc ccgcccgcac cccgtccgcc gccctcgtcg tgcccgattg
     3421 ctgcaacatc ggcgtggtat tccgcatcct gctcgtggtg aacgcggccg tgttctgcgc
     3481 ggccttgctg cagtcgccga gcgtgggcgc cggcgtgcag gcctttgtcg acgcgagcat
     3541 ggtggtcgag cttgcctgcc tgtggtccct gttcgcggtg tgcatattgc ggcggctgct
     3601 gctgcgcgcg cgggatggct atgcaa
//
LOCUS       DATLGF010000431         5308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_233081, whole genome shotgun sequence.
ACCESSION   DATLGF010000431 DATLGF010000000
VERSION     DATLGF010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233081"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VM406_13140"
     CDS             <1..261
                     /locus_tag="VM406_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HVL76953.1"
                     /translation="LQGAAGWKEPRLRAILQNTIRRKPGRTEFLRGVLSGDLESGLQV
                     RLTGAQGSGILSSMAQANCIVVLAPERGEVAAGEPVEVVLLD"
     gene            complement(287..1603)
                     /locus_tag="VM406_13145"
     CDS             complement(287..1603)
                     /locus_tag="VM406_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HVL76954.1"
                     /translation="MEIALLIGLILLNGGFAMSEIALVTARRARLQRLAESGDASALA
                     AIRLGEDPNQFLSTVQVGITSIGILNGIIGQAVLAQPIAQWLQRGGLQSPFADYLATG
                     LVVVTITYVTIVLGELVPKRLGQISPEAIARLVARPMILLASATRPFVRLLAASTQIV
                     LRSFGVSERAEPTVTQEEINLVLAEGSSAGVIEQHEHQMVRNVFRLDDRQIASLMVPR
                     SDVVYLDVNEPLEVNLKRFEESEHSRFPVVRGGWNEVLGFVNARQLLLQMLRGEKLNL
                     TDSLLPAVFVPESLTGMELLENFRNSGVQLAFIIDEYGEVQGIVTPQDVMEAITGEFK
                     THRVEDAWAVQREDGSWLLDGLIPVPELKDRLGLHGVPEEERGRYNTLSGMLMLLLGR
                     LPVTGDRCDWENWNFEIVDLDGKRIDKVLATAKPVAPPERIEPIAG"
     gene            complement(1686..3137)
                     /gene="rmuC"
                     /locus_tag="VM406_13150"
     CDS             complement(1686..3137)
                     /gene="rmuC"
                     /locus_tag="VM406_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HVL76955.1"
                     /translation="MTAFELILLLLLGAVLVLQLVSLFRARAADVNDALLRLQNDMQR
                     QQQAADGQARELRGEVHASAQGTRQELGANFAQFQQALTAQLTSVATLQNKQIDSFAQ
                     QLAQWNGSSSQQLDAMRQAIASQAQAGREEQAAALKRFGDTLNQALGSLTESNAQRMA
                     EVRATLETKIRELQTDNAARLEEMRRTVDEKLHATLEQRLGESFKLVSDRLEKVHQGL
                     GEMQQLAVGVGDLKRVLTNVKTRGTWGEVQLGMLLEQVLTPDQYTKNVETVPGSGERV
                     EFAVRLPGQEASERPVWMPIDAKFPKEQYERLVEAAELADAEGVAQAGRELERAVRVE
                     AKTISEKYLSPPLTTDFAILFLPTEGLYAEVMRRPGLADELQRNCRVAIAGPSTLSAL
                     LNSLQMGFRTLALEKRSSEVWQVLGAVKTEFGKFGEVLATTKATLERAARNIEQAETR
                     TRQMSRKLKSVEALPAESAKMLLGTDGVDTADE"
     gene            complement(3159..3557)
                     /locus_tag="VM406_13155"
     CDS             complement(3159..3557)
                     /locus_tag="VM406_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HVL76956.1"
                     /translation="MTIQDDQATAFELIGGEDRLRELVDRFYDLMELEPGFAALRAMH
                     PPRLEDSRDKLFWFLCGWTGGPNHYIERFGHPRLRARHLPFSIASAERDQWLRCMALA
                     MQDIGLSEPLQERLMHAFFQTADWMRNTAG"
     gene            3816..5222
                     /locus_tag="VM406_13160"
     CDS             3816..5222
                     /locus_tag="VM406_13160"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HVL76957.1"
                     /translation="MRDLIITNSSRLEFSVRVMDVLVLQVAGLLAAIAHFQMPISEAP
                     PIHLVLLHTCSALAFLLFPQFDLYTSWRGRSLSDLFLRLAGAWAVVLLLGLFFSYLIH
                     HVGLASRMWMFYWYATGITLLVVYRVGVYYALRYLRMKGMNNKRVVIVGYGSTGQEMH
                     RRALQQDWTGYEVKAVHADCIDAQQLKDPSIRRLENLEDLHDYVEANHIHEIWITLPL
                     ADSAKLQELQYVMRNTLVDIRWVPDVLGLQLLSHRMDNFLGLPVVDLNQPISSGVSGV
                     SKDIFDKVFAACALVMLIPLFFVLAILIKASSPGPVFFKQPRLGLNGRPFNVYKFRTM
                     KVHKEDKRVTQATRNDPRITPIGAFLRRTSLDELPQFFNVLMGDMSVVGPRPHAIEHN
                     EMYKDLLEMYMLRHRVKPGITGWAQIHGYRGETDTVDKMAARVQFDLDYIQNWSLWMD
                     IRIIVWTAFKGWTGKNAY"
BASE COUNT          905 a         1746 c         1700 g          957 t
ORIGIN      
        1 ctgcagggcg cggccggctg gaaggagccg cgcctgcgcg caatcctgca aaacacgatc
       61 cgcaggaaac ccgggcgcac cgagttcctg cgcggcgtgc tgtccggcga cttggaaagc
      121 ggattgcagg tgcgcctgac cggcgcgcag ggctcgggca tcctgagctc gatggcccag
      181 gcgaactgca tcgtcgtgct ggcgccggaa cgcggcgagg tggcggccgg cgagccggtg
      241 gaagtggtgc tgctggactg agaagccctg accccgcttg cggcgatcag ccggcgatcg
      301 gctctatcct ctccggcggc gccacaggct tggccgtggc cagcaccttg tcgatgcgct
      361 tgccatccag atcgacgatt tcaaaattcc agttctccca atcgcagcgg tcgccggtga
      421 ccggcaggcg gcccagcagc agcatcagca ttcccgaaag cgtgttgtag cgtccgcgct
      481 cttcctccgg cacgccgtgc agcccgaggc ggtccttcag ctcggggacc ggaataagtc
      541 cgtcgagcag ccaggagccg tcttcgcgct gcaccgccca ggcgtcttcg acgcggtgcg
      601 tcttgaattc cccggtgatc gcttccatca cgtcctgtgg cgtgacgatg ccctggactt
      661 cgccgtactc gtcgatgatg aacgcgagct gcacgccgga attgcggaag ttctccagca
      721 gctccatgcc ggtcagcgac tcgggcacga acacggccgg cagcaggctg tccgtcaggt
      781 tcagcttctc gccacgcagc atttgcagca gcaactggcg cgcattgacg aaaccgagca
      841 cctcgttcca gccgccgcgc acgaccggaa agcgcgaatg ctcgctttcc tcgaagcgct
      901 tcaggttcac ttccagcggc tcgttgacgt cgagatacac gacatcgctt ctgggcacca
      961 tcagcgaggc gatctggcgg tcgtcgagac ggaacacgtt acgcaccatc tggtgctcgt
     1021 gctgttcgat gacgccggcg ctcgatccct cagcgaggac gaggttgatc tcttcctggg
     1081 taacggtagg ctcggcgcgc tcggacacgc cgaagctgcg cagcacgatc tgcgtcgacg
     1141 cggcaagcaa gcgcacaaac ggtcgcgtgg cgctggccag caaaatcatc ggacgcgcaa
     1201 ccagtcgcgc gatcgcctcc gggctgatct ggccgagccg cttaggcacg agttcgccca
     1261 gcacgatggt gacgtaggtg atggtgacga cgaccaggcc ggttgcaaga tagtccgcga
     1321 acgggctctg caggccgccg cgctgcagcc actgggcgat cggctgggca agcacagcct
     1381 ggccgatgat gccgttcagg atgccgattg aggtgatgcc gacctgcacc gtcgagagaa
     1441 actggttcgg gtcctcgccg aggcgaatgg cggccagcgc cgaggcgtca ccggattcgg
     1501 ccaagcgctg cagccgcgcg cggcgggcgg tgacgagcgc gatctcggac atcgcgaagc
     1561 cgccgttgag gaggatgagg ccgatcagaa gtgcgatttc catggcatag ggtctcgctg
     1621 ctgaaactca ggcacgcgca tgagggggcg cgtgccgccg ccgttcaact ttatccgggt
     1681 acgcgtcatt catcggccgt gtcgacgcca tcggtcccca gcagcatctt cgccgactcc
     1741 gccggcagcg cttcgacgga cttgagcttg cgcgacatct ggcgcgtgcg cgtttccgcc
     1801 tgctcgatat tgcgcgccgc acgctcgagc gtggccttgg tcgtggccag cacctcgccg
     1861 aacttgccga actcggtctt caccgcgccg agcacctgcc agacttcgga cgagcgcttt
     1921 tccagcgcca gtgtacggaa acccatctga aggctgttga gcagggccga cagcgtggac
     1981 ggaccggcaa tggccacgcg gcagttgcgc tgcagttcgt cggccaggcc gggacggcgc
     2041 atcacctcgg cgtacaggcc ctcggtgggc aggaacagga tcgcgaagtc tgtcgtcagc
     2101 ggcggcgaca gatacttttc cgagatggtc ttggcttcga cgcgcaccgc gcgctccagc
     2161 tcgcgaccgg cctgcgcgac gccttcggca tcggccagtt cggctgcctc aaccaggcgt
     2221 tcgtactgct cctttgggaa cttggcgtcg atcggcatcc agaccggccg ttcgctggct
     2281 tcctggccgg gcaggcgcac ggcgaactcg acccgctcgc cggaccccgg aacggtttcc
     2341 acgttcttcg tgtactggtc cggcgtgagc acctgctcga gcagcatgcc gagctggact
     2401 tcgccccagg tgccgcgcgt cttgacattg gtcagcacgc gtttcaagtc gccgacgcca
     2461 accgcgagct gctgcatctc gcccagcccc tggtgcacct tctccagccg gtccgacacg
     2521 agtttgaacg actcgccgag gcgctgctcg agcgtggcgt gcagcttttc atcgacggtg
     2581 cggcgcatct cctcgagccg cgcggcattg tcggtctgca gttcgcggat cttggtttcc
     2641 agcgtcgcgc gcacctcggc catgcgctgc gcattcgatt cggtcaggct gcccagcgcc
     2701 tggttgaggg tatccccgaa acgcttgagc gcggctgcct gctcttcgcg gccggcctgg
     2761 gcctggctgg cgatcgcctg gcgcatcgca tcgagctgct gggagctgga gccgttccac
     2821 tgcgcgagct gctgggcgaa ggagtcgatc tgcttgttct gcagcgtggc gacgctggtg
     2881 agctgggctg tcagggcttg ctggaactgg gcgaagttcg cgccgagttc ctgccgcgtg
     2941 ccctgggcgc tggcgtgcac ctcgccgcgc agctcgcgcg cctggccgtc tgccgcctgc
     3001 tgctggcgct gcatgtcgtt ctgcagacgc agcagcgcgt cgttcacatc cgccgcgcgg
     3061 gcgcgaaaca gggagacaag ctgcagcacg agcaccgcgc ccagcagcag caggaggatc
     3121 agttcaaaag cggtcatgtc gaaatcagtg ggatgggctc agccggccgt gttgcgcatc
     3181 cagtcggccg tctggaaaaa ggcgtgcatc aggcgctcct gcagcggctc ggacaggccg
     3241 atgtcctgca tggccagtgc catgcagcgc agccactggt cgcgctccgc ggaagcgatc
     3301 gaaaagggaa gatggcgcgc gcgcaggcgc ggatggccga agcgttcgat gtagtggttg
     3361 ggcccgcccg tccagccgca caggaaccag aacagcttgt cgcgcgaatc ctccaggcgc
     3421 ggcggatgca tcgcgcgcag cgccgcgaac ccgggctcca gttccatcag gtcatagaag
     3481 cggtcgacca gttcgcgcag cctgtcctcc cctccgatca gttcgaatgc tgtcgcttgg
     3541 tcatcttgaa tcgtcatggc gggaatgata gcgcatcgcg gcccgctaca agaagccggt
     3601 aacggcttga tctgaagccg tattctgccc gcgcgccggg cagcgcttga gcttgcacaa
     3661 cgcggcgatt gcgacctcac aacgggccga ttgagacgtc gcaagcatcg aacagttagg
     3721 gatgcggcgc gcgccacagt cggtaccatt gcttcaaaga caacacctgt tctccccgaa
     3781 ccggtgccgt atcgaagcaa aaacgaagaa gacacatgcg cgatctgatc atcaccaact
     3841 cttcccgcct ggaattctcg gttcgcgtga tggacgtgct ggtcctgcag gtcgccgggt
     3901 tgctggccgc gatcgcccat ttccagatgc cgatctccga ggccccgccg atccacctgg
     3961 tcctgctgca tacctgcagc gcgctcgcgt tcctgctgtt cccccagttc gatctctaca
     4021 cgtcctggcg cggccgctcg ctgtccgacc tgttcctgcg cctggccggc gcctgggcgg
     4081 tggtactgct gctcgggctg ttcttcagct acctgatcca ccacgtcggc ctcgcctcgc
     4141 gtatgtggat gttctactgg tacgcgaccg gcattacgct gctggtggtg taccgcgtcg
     4201 gcgtctacta cgcattgcgc tatctgcgca tgaagggcat gaacaacaag cgcgtggtga
     4261 tcgtcggtta cggcagcacc ggacaggaga tgcaccggcg tgcgctgcag caggactgga
     4321 ccggctacga ggtcaaggca gtccatgccg attgcatcga cgcgcaacag ctgaaggacc
     4381 cgtccatcag gaggctggag aacctggaag acctgcacga ctacgtcgaa gccaatcaca
     4441 tccacgaaat ctggatcacc ctgcccctgg cggactcggc caagctgcag gagttgcagt
     4501 acgtgatgcg caatacgctg gtcgacatcc gctgggtgcc ggacgtgctt ggcctgcagc
     4561 tgctgtcgca ccggatggac aatttcctcg gcctcccggt ggtcgacctg aaccagccga
     4621 tttcaagcgg cgtgtccggc gtgtccaagg atatcttcga caaggtgttc gccgcctgcg
     4681 cgctggtcat gctgattcca ctgttcttcg tgctggcgat cctgatcaag gcgagctcgc
     4741 cgggcccggt tttcttcaag cagccgcgcc tgggcctgaa cggccgcccg ttcaacgtct
     4801 acaagttccg cacgatgaag gtgcacaagg aagacaagcg cgtcacgcag gccacccgca
     4861 acgatccgcg catcacgccg atcggggcct tcctgcgccg caccagcctg gacgagctgc
     4921 cgcagttctt caacgtgctg atgggcgaca tgtcggtggt tggcccgcgc ccccatgcga
     4981 tcgaacacaa cgagatgtac aaggacctgc tcgagatgta catgctgcgc caccgcgtca
     5041 agcccggcat taccggatgg gcgcagatcc acggctaccg cggcgagacg gacacggtcg
     5101 acaagatggc cgcgcgggtg cagttcgacc tcgattacat ccagaactgg tcgctctgga
     5161 tggacatccg catcatcgtc tggaccgcat tcaagggctg gaccggaaag aacgcgtact
     5221 gaaaggacca cccgcatttc cacggagttg catgaaggaa taccgtccca tctcatctct
     5281 gcgcgacgcg ctctcgctgc tcacgccc
//
LOCUS       DATLGF010000432         5688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_215351, whole genome shotgun sequence.
ACCESSION   DATLGF010000432 DATLGF010000000
VERSION     DATLGF010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215351"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="VM406_13165"
     CDS             <1..1333
                     /locus_tag="VM406_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HVL76958.1"
                     /translation="VGASDDDLIALAGRGERNWWLRLLAAGETASPALARAARLLAQW
                     LEAAPRLPVHDLLDMVMHEGDLVARYAAAAHPARRAQVLGNLEAFMELALELDSGRYP
                     SLPRFIEQLRLLRNGTENAAPDEASIDAAADAVRILTIHSAKGLEAPIVALLDANHSD
                     PAREDCGILCDWPQDADGPTHFSCFGRTSERGAARDALFAAEEGYAAQEDWNLLYVAI
                     TRARDVLLLTGVANAKSDDGTKDNSWYARMAGVPVLDPETLPEPVLAAAQTGEAFSLM
                     LFDPAPLPAPAAVVPTQVSSAAIEEGVALHALLERLTQDGTWPPAVPSVAEIGRWIGC
                     GRELAQKVRAQAECILGAPALERFFNPAHFTRAHNELEVVVDGMLLRMDRLVVSDDAL
                     WVLDYKRDVLDSERSAYAQQVERYLRALAPIFPGMRMYGGLIRSDGVLIEL"
     gene            complement(1500..2513)
                     /gene="cheB"
                     /locus_tag="VM406_13170"
     CDS             complement(1500..2513)
                     /gene="cheB"
                     /locus_tag="VM406_13170"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006497325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HVL76959.1"
                     /translation="MRIGIVNDVALIAEALRRLIVNTREHEVIWVAHGGLQALRLCAE
                     QRPDLVLMDLIMPDIDGAEATRRIMTEAPCPILIVTASPDENTNLVFRAMGAGALDVV
                     ATPVISGRAGGDGALLAKIRTIGKLNGALVAPVTPSPRPLAPRVLPATAGVSTLVAIG
                     ASTGGPHALAQIFARWKPPAHCCTVIVQHIDEGFADSFAHWLAEQIRHPVAAIRDGAA
                     LAPGAILLARTNDHLTLSAEHRLRYSVEPSGYPYRPSVDVFLRAVALHWRKPAIGVLL
                     TGMGRDGAHGLLAMRMAGKTTIAQDRASSAVFGMPRAAAELGAAQEIAAPEAIARLLA
                     ERV"
     gene            complement(2510..4732)
                     /locus_tag="VM406_13175"
     CDS             complement(2510..4732)
                     /locus_tag="VM406_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL76960.1"
                     /translation="MSSDEEDGGVSLSELFCAEAQAQARVLGDGLLRLEREEPGAAAI
                     EPLMRAAHSLKGAARVLDMSDIEQLAHVMEDCFLAASRGSMRLERGRVDRLLAAVDLI
                     AEMAAAGASQVGAWLARNQGRIADATLALHELAHAADTVQAPPSPAPPSTANPPAPPA
                     VSAAADSALRIDARALDRLLALAGEARVAGNALGPQKAALQRLRQRQHEAFTLLDHLQ
                     LSVRTNAAAELKNLRERMAPLEQMLAERVAELEAFERRLQGLASGLLDEVLALRMRPF
                     RDATQGLARMVRDLAHTLGKEARLEIAGGDTPVDRDVLTGIEAPLQQALRNAVDHGIE
                     LPAQRHAAGKPQAGSVRLEARHRGGMLEVVVSDDGQGLDPERIREVVVQRGLCALPMA
                     EAMSEAELYDFLLLPGFSLKESAGTISGRGVGLDVVADAVRRLDGRLRLASTPGRGFM
                     LHIVLPLSRSLMRALVFRVAGQPFALPLAHVLRVARVPRAQVHTLAEREFFTLAGQHI
                     DLIAAAPLLEIGAAAAQEHALAVLVIGQGGRKFGLVVDAIDGEESVAVQPLDGLFGKL
                     RDVAGAAVLENGTPALVLDHADLIHSIERLAQAAGLRNVPGPAVQRPALARRILVVDD
                     SLTVREMERRLLQSRGYQVEVAVDGMDGWNAVRSGDFDLVITDVDMPRLDGIELVSRI
                     KKDERLRNLPVMIVSYKDRAEDRMRGVEAGADYYLAKGSFHDETLLDAVADLIGDSAE
                     "
     gene            complement(4729..5403)
                     /locus_tag="VM406_13180"
     CDS             complement(4729..5403)
                     /locus_tag="VM406_13180"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HVL76961.1"
                     /translation="MNEHGIDACWNRIGVHGDQSCTRLAEHGHCRRCEIFADAARRAM
                     HREIPPGYRRTWAAQFAVPSSTVVPQAGRRRAMLVFRLAQEWLGLPAASVECVTEPVE
                     ALRLPHRPGPVLLGIAAIRSKLHPCMSLAALLGIEGAAPARGAGLRVHACTLLLRLAR
                     ATVAVPVHEVLGVVRLNDEDLEPPPTSLDLDAPSCIVGVFSHEDKRIGCLDADLLARN
                     LERQLQ"
     gene            complement(5400..>5688)
                     /locus_tag="VM406_13185"
     CDS             complement(5400..>5688)
                     /locus_tag="VM406_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVL76962.1"
                     /translation="GHGEQARRLLQRSLRLHPDSAHAHFMLALLSEREQDIGAAEDAL
                     RRAVYLDPDHYEALCHLAMLSERHGDAGRAARLRDRAARVYRRRELKGHRR"
BASE COUNT          855 a         1952 c         1996 g          885 t
ORIGIN      
        1 cgtcggcgcg tccgacgacg acctgatcgc gctggccggc cgcggcgagc gcaattggtg
       61 gctgcgcctg ctggccgcgg gtgagacggc gtcgcctgcg ctcgcgcgtg cggcacggct
      121 gctggcgcag tggctggaag cggcgccgcg tctgccagtg cacgatctgc ttgacatggt
      181 gatgcacgag ggcgacctgg tggcgcgtta tgcggcggcc gcgcatcccg cgcggcgcgc
      241 gcaggtgctg ggcaacctgg aagcattcat ggagctggcg ctggaactcg attcggggcg
      301 ctatccgagc ctgccgcgct tcatcgagca gctgcgcctg ctgcgcaacg gcacggagaa
      361 tgcggcgccg gacgaagcca gcatcgatgc ggcggccgat gcggtgcgca tcctcacgat
      421 tcacagcgcc aagggcctgg aagcgccgat cgtcgcgctg ctcgatgcga accacagcga
      481 tcccgcgcgc gaggactgcg gcatcctgtg cgactggccg caggatgcgg acggtcccac
      541 gcacttctcc tgcttcggcc gtacttccga gcgcggggcc gcgcgcgatg cgctgttcgc
      601 ggccgaggaa ggctacgccg cgcaggagga ctggaacctg ctgtacgtgg cgatcacccg
      661 tgcgcgggac gtgctgctgc tgaccggcgt ggcgaacgcg aagtccgacg acggcaccaa
      721 ggacaacagc tggtatgcgc ggatggcggg cgtgccggtg ctggatccgg aaacgctgcc
      781 cgagccggtg ctggccgctg cgcagacggg agaagcgttc tcgctgatgc tgttcgaccc
      841 ggcgccgctg ccggctccgg cggcggtggt gccgacccag gtttccagcg ccgcgatcga
      901 ggagggcgtt gcactgcatg cgctgctcga acgcctgacg caggacggga cctggccgcc
      961 ggccgtgccg tcggtggcgg agatcgggcg ctggatcggc tgcggtcgcg agcttgcgca
     1021 gaaggtgcgc gcgcaggccg aatgcatact cggcgcgccg gcgctcgaac gcttcttcaa
     1081 tcccgcgcac ttcactcgcg cacacaacga gcttgaagtg gtggttgacg gcatgctgct
     1141 gcgcatggac cggcttgtcg tgagcgacga cgcactgtgg gtgctcgact acaagcgcga
     1201 cgtgctcgac agcgaacgga gcgcgtatgc gcagcaggtc gaacggtatc tgcgggcgct
     1261 ggcgccgatc ttcccgggca tgcggatgta cggggggctg attcgttcgg acggtgtgct
     1321 gattgagctg tagcggtggt ggtgcgaagg acgctggcgc ggcatggtgc tgtgctggga
     1381 ttgcatccca gcctacggtg ctatggtgct gcggaggagg taggctggga tgaaatccca
     1441 gcaaagttcg tcccggtgaa cgccgggcta cagcgacgta ccgcaaaggc tcgtttacat
     1501 cacacccgct ccgcaagcag acgcgcgatg gcttccggtg ccgcgatctc ctgcgccgcg
     1561 cccagttccg ctgccgcacg cggcatgccg aacaccgcgc tgctggcacg gtcctgggcg
     1621 atggtggtct tgcccgccat gcgcatcgcc agcaggccgt gcgcgccgtc gcgtcccatt
     1681 ccggtcagca gcacgccgat tgccggcttg cgccagtgca gcgcgaccgc gcgcaggaac
     1741 acatcgacgg acggacgata cggatagccg gatggttcga cgctgtagcg cagccggtgc
     1801 tcggcgctga gcgtcagatg gtcgttggtt cttgccagca ggatcgcgcc gggggcaagg
     1861 gcggcgccgt cgcggattgc ggcaaccggg tgacgaatct gctcggccag ccagtgcgcg
     1921 aagctgtcgg caaagccttc gtcgatgtgc tgcacgatca ccgtgcagca gtgcgcgggc
     1981 ggcttccagc gcgcgaagat ctgcgcaagc gcgtgcggac ctccggtgga cgcgccgatg
     2041 gccaccagcg tgctgacgcc ggccgttgcc ggcagcacgc gcggcgcgag cgggcgcggg
     2101 ctcggcgtga ctggcgcgac gagggcgccg ttgagtttgc cgatcgtgcg gatcttcgcc
     2161 agcagcgcgc catcgccgcc ggcgcggccg ctgatcaccg gcgtggcgac cacgtcgagc
     2221 gcgcctgcgc ccatcgcacg gaacacgaga ttggtgttct cgtcgggcga ggcggtgacg
     2281 atcaggatcg ggcagggcgc ctcggtcatg atgcgccgcg tggcttcggc gccgtcgatg
     2341 tccggcatga tgaggtccat cagcaccagg tccgggcgct gttccgcgca caagcgcagg
     2401 gcctgcaggc cgccgtgggc aacccagatg acttcatgtt ctcgcgtgtt gacgatcagg
     2461 cggcgcaggg cttcggcaat cagggcgaca tcgttgacga ttccgattct cactctgcgc
     2521 tgtctccaat caggtcggcc accgcatcca gcagggtctc gtcatggaag ctgccctttg
     2581 ccaggtagta atcggcgccg gcttcgacgc cgcgcatcct gtcctccgcg cggtccttgt
     2641 atgagacgat catcaccggc aggttgcgca gtcgttcgtc cttcttgatc cggctcacga
     2701 gctcgatgcc gtccagacgc ggcatgtcca catcggttat caccaggtcg aaatcgccgc
     2761 tcctgacggc gttccagccg tccatgccat ccaccgccac ctcgacctga tagccgcgcg
     2821 attgcaacag ccggcgctcc atttcccgta cggtaagcga gtcgtcgacc accaggatcc
     2881 gcctggcaag cgcgggccgc tgcacggccg gtccgggcac gttgcgcagg ccggcggcct
     2941 gcgcgagccg ctcgatgctg tgaatcaggt ccgcgtggtc gagcacgagt gccggcgtgc
     3001 cgttttccag cacggcggca cctgcgacgt cgcgcagctt tccgaacagg ccgtcgagcg
     3061 gctgcacggc cacgctctct tcgccgtcga tcgcatcgac gaccaggccg aacttccggc
     3121 cgccctggcc gatgaccagg accgcaagcg catgctcttg cgcggcggcc gccccgattt
     3181 ccagcagcgg cgcggcagcg atcaggtcga tatgctggcc cgcgagcgtg aagaactcgc
     3241 gttcggcaag cgtgtgcacc tgcgcgcgcg gcacccgcgc aacacgcagg acgtgggcga
     3301 gcggcagcgc gaacggctgg ccggccacgc gaaacaccag cgcgcgcatc agcgagcgcg
     3361 acagcggcag cacgatgtgc aacatgaatc cccggcctgg cgtgctggcc agccgcagcc
     3421 ggccatcgag ccgccgcacg gcgtcggcga cgacatccag cccgaccccg cggccggata
     3481 tggttcccgc cgattccttc aggctgaaac cgggcagcag cagaaagtcg tacagctccg
     3541 cctcgctcat cgcttcggcc atcggcagcg cgcacaggcc gcgctgcacg acgacttcac
     3601 ggatgcgttc cgggtccagg ccctggccgt cgtcgctgac caccacttca agcatgccgc
     3661 ctctgtggcg cgcctccagc cgcacggaac ctgcctgcgg cttgccggcc gcgtgccgct
     3721 gcgcggggag ttcgatgccg tggtcgaccg cattgcgcag cgcctgctgc agcggtgcct
     3781 cgatgccggt gagcacatcg cggtcgaccg gtgtgtcgcc gccggcgatc tccaggcgcg
     3841 cctccttgcc cagcgtgtga gccagatcgc gcaccatgcg cgcaagaccc tgcgtggcgt
     3901 cccggaacgg acgcatgcgc agcgcaagga cctcgtcgag caggcctgag gcaagcccct
     3961 gcagccggcg ttcgaatgct tcaagctcgg ccacccgctc cgccagcatc tgctccagcg
     4021 gcgccatgcg ctcgcgcagg ttcttcagct ctgcggccgc attggtccgg acggacagtt
     4081 gcaggtgatc gagcagcgtg aacgcttcat gctgacgctg gcgcagtcgt tgcagcgccg
     4141 ccttctgcgg gccgagcgca ttgcccgcca cgcgcgcttc gccggccagt gcaagcaggc
     4201 ggtcgagtgc acgcgcatcg atgcgcagag ccgaatcggc cgcggcggat acagccggcg
     4261 gggcaggtgg attagcggtg cttggcgggg caggggatgg cggcgcttgc actgtgtcag
     4321 cggcgtgcgc aagctcatgc agcgcaagcg tggcatcggc gatgcggccc tgattgcgcg
     4381 cgagccaggc gccgacctgc gacgcgccgg ctgccgccat ctcggcgatc aggtcgaccg
     4441 ccgcaagcaa acggtcgacg cggccgcgtt ccagacgcat gctgccgcgg ctggccgcga
     4501 ggaaacagtc ctccatcaca tgcgcgagct gctcgatgtc ggacatgtcc agcacacgcg
     4561 cggcgccctt gagcgagtgg gcggcgcgca tcagcggctc gatggcagcc gcgcccggct
     4621 cctcccgttc cagccgcagc agcccgtccc cgagcacgcg cgcctgcgct tgcgcttccg
     4681 cgcagaacag ctcggacagg gagacgccgc cgtcttcttc gtcactgctc attggagttg
     4741 ccgttccagg ttgcgggcga gcaggtcggc atccagacag ccgatgcgct tgtcctcgtg
     4801 gctgaacacg ccgacgatgc aggacggcgc gtccaggtcc agcgaggtcg gcggcggctc
     4861 cagatcctcg tcgttcaagc gcaccacgcc aagcacctcg tgcaccggca ccgcgactgt
     4921 cgcacgagcc aggcgcagca gcagcgtgca cgcgtgcacg cgcaagccgg ctccgcgcgc
     4981 gggcgccgcg ccctcgatgc cgagcagggc cgccagcgac atgcacggat ggagcttgct
     5041 gcgaatggcg gcgatgccga gcaggaccgg gccgggccgg tgcggcaggc gcagcgcctc
     5101 tactggttcg gtcacgcatt ccaccgacgc agcaggcaag ccgagccatt cctgtgcgag
     5161 tcgaaatacc agcatcgcgc ggcgcctgcc agcctgcggt accaccgtcg aggacgggac
     5221 ggcgaattgt gcggcccagg tccggcggta gccgggaggt atctcgcgat gcatcgcccg
     5281 tcgcgccgca tcggcgaaga tctcgcagcg gcggcaatgg ccgtgctccg ccaaccgcgt
     5341 gcagctctga tcgccgtgca cgccgatccg gttccagcat gcgtcgatgc cgtgctcgtt
     5401 catcgccggt gtcctttcag ctcgcgccgc ctgtataccc gagcggcccg gtcgcgcagc
     5461 cgggccgccc ggccagcgtc gccgtgccgt tcgctgagca tcgcgagatg gcacagcgct
     5521 tcgtaatgat ccgggtcgag ataaactgcg cgccgcagcg cgtcttcggc cgcaccgatg
     5581 tcctgttcgc gttcactgag cagggccagc atgaagtgtg catgcgcgga atccggatgc
     5641 aggcgcaggc tgcgttgcag caggcgccgc gcctgctcgc cgtgcccg
//
LOCUS       DATLGF010000433         5853 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_76922,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000433 DATLGF010000000
VERSION     DATLGF010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5853
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76922"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VM406_13190"
     CDS             <1..269
                     /locus_tag="VM406_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HVL76963.1"
                     /translation="DALPTGPALAARQPELRRRFALAGGDDYELCFTAPADRRDAVLA
                     AARASETVVTRIGRIESGPGLRLTDGNGAPLVLDTTSFDHFASS"
     gene            266..784
                     /locus_tag="VM406_13195"
     CDS             266..784
                     /locus_tag="VM406_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HVL76964.1"
                     /translation="MNPPEQSTMLRKPDSRFMLAHPAHWIAQGFGSGLAPALPGTFGT
                     LFGWLVFVVLTARWPEFFTAANWAIIIVVSFVVGIWACDKTGRDLGIADHGSMVWDEI
                     VAMWIVLLFIMPAGLATQIWAFLWFRFFDMLKPPPIGWFDRRLKGGFGVMWDDVVAAF
                     YTLLLFAVWRTL"
     gene            781..4383
                     /locus_tag="VM406_13200"
     CDS             781..4383
                     /locus_tag="VM406_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HVL76965.1"
                     /translation="MSARWEFWVDRGGTFTDIVARRPDGSLLTHKLLSEDPRYADAAV
                     AGIRHILGLSSGEPIPAQLIESVKMGTTVATNALLERKGDPTLLVITKGFRDALRIGY
                     QNRPRLFDRHVVLPELLYRDVIEADERIDAHGGVVAALDTERVRQDLQQRHTDGYRAV
                     AIVLMHGYRYPEHERAIAAIARDLGFTQVSVSSEVSPLMKLVSRGDTTVVDAYLSPIL
                     RRYVDQVASELRGVRLLFMQSNGGLTDARRFQGKDAILSGPAGGIVGMARTAESAGFD
                     RIIGFDMGGTSTDVSHYAGEFEREFDTQVAGVRLRAPMMSIHTVAAGGGSVLHFDGTR
                     FRVGPDSAGASPGPACYRRGGPLAVTDCNVMLGKIQPNHFPAVFGDMGNQTLDREIVA
                     QKFSALAQDIFRATGQRRTPEEVAEGFIEVAVGNMANAIKFISVQRGHDVTGYTLCTF
                     GGAGGQHACLVADALGMTRVFAHPLAGVLSAYGMGLADQTAMREQALEAPLGEAAMPA
                     AQAHLNALAEAARADLLAQGVEAARIRVVERAHLRYSGTDSAIVVPFGSVADMTARFE
                     AAYQRRYSFLMQDKGLVIEALSVEAIGVADDVAAHAEVLPERVGRLGPAATVRMFSGG
                     RWHDAPLYLRADIRPGDHIAGPAIIAEKNATTVVEPGWDAEVTPLNHLVLERVEARPA
                     RKAIGTSADPVMLEVFNNLFMSIAEQMGLRLQNTAFSVNIKERLDFSCALFDAHGNLI
                     ANAPHIPVHLGSMGESIKTVIRENGATMRAGDVFMLNDPYHGGTHLPDVTVITPVFDR
                     AGANVLFHVASRGHHADIGGTTPGSMPPDSTIVEEEGVLIDNFQLVRAGRLLEQETVA
                     LLSSGRYPARNIRQNLADLQAQIAANQKGADELLKMVDHFGLEVVHAYMRHVQDNAEE
                     AVRRVISALKDGRYTYRLDNGAVIEVAIRVDRAARSADIDFTGTSPQLQNNFNAPSAV
                     CMAAVLYVFRTLVDDDIPLNAGCLKPLNVIIPEGSMLNPHYPAAVVSGNVETSSCVTN
                     ALYGALGVMASAPGTMNNFTFGNERHQYYETISGGSGAGDGFDGTDVVQTHMTNSRLT
                     DPEVLEWRFPVRLESYEIRHGSGGAGRWRGGNGGVRRIRFLERMTASILSNNRVVPPF
                     GAAGGGDGQCGRNYVIRVDGRVETLGPTARTELEPDDVFVIETPGGGGFGQAQD"
     gene            4466..4951
                     /locus_tag="VM406_13205"
     CDS             4466..4951
                     /locus_tag="VM406_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HVL76966.1"
                     /translation="MSDKIELARRVGQALQERGWLLAVAESCTGGGVGQAVTEIAGSS
                     EWFDCGFIVYSNASKTELVDVPAAMIAQYGSVSEEVAGAMAEGALANSNAHVALSTTG
                     IAGPAGAVPGKPVGTVCFGWAAEHRTYTERHVYSGDRQSVREQTIVHALEGLLRFLEQ
                     K"
     gene            complement(4958..5776)
                     /gene="pyrF"
                     /locus_tag="VM406_13210"
     CDS             complement(4958..5776)
                     /gene="pyrF"
                     /locus_tag="VM406_13210"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HVL76967.1"
                     /translation="MTFTEQLSAAWARNDSLLCVGLDPDVSRFPAELAGRPQAVFEFC
                     KAIADATADVACAFKPQIAYFAALRAEDQLEALCAHLRSAHPHIPIVLDAKRGDIGAT
                     AEQYAREAFERYGAHAVTVNPYMGFDSVQPYLEWKDRGVIVLCRTSNPGGSDLQFLTA
                     DGMPVYQHVARLVAQKWNTNGQCGLVVGATYPQELAEVRRIVGDMPLLVPGVGAQGGD
                     VEATVKAGRTTAGLGMMINSSRAILYAKAEAGERFTDAARRVAIETRDAINRYR"
BASE COUNT          967 a         2017 c         1935 g          934 t
ORIGIN      
        1 cggatgcgct gccgaccggc cctgcccttg ccgcacggca gcccgagctg cgccggcgct
       61 tcgcgttagc aggcggcgac gattacgagc tgtgcttcac cgcccccgca gaccggcgcg
      121 acgccgtcct cgccgccgcc cgcgcgagcg agacggttgt cacgcgcatc ggccgcatcg
      181 aatccggccc gggcctgcgc ctgaccgacg gcaacggcgc cccgctcgtc ctcgatacca
      241 cttccttcga ccatttcgcc tcgtcatgaa tccacccgaa caaagcacga tgctgcgcaa
      301 gccggactcc cgcttcatgc tggcccatcc ggcccactgg atcgcgcagg gcttcggcag
      361 cggcctggcc ccggccctgc ccggcacctt cggcacgctg ttcggctggc tggtcttcgt
      421 ggttctcacc gcacgctggc cggagttctt caccgccgcg aactgggcga tcatcatcgt
      481 cgtgtccttc gtagtgggca tctgggcctg cgacaagacc ggccgcgacc tcggcatcgc
      541 cgaccatggc agcatggtgt gggacgagat cgtcgcgatg tggatcgtgc tgctcttcat
      601 catgccggcc gggctggcta cgcagatctg ggcattcctc tggttccgct tcttcgacat
      661 gctcaaaccg ccgccgatcg gctggttcga ccgccgcctg aagggcggat tcggtgtgat
      721 gtgggacgac gtggtcgccg ccttttacac gctgctgctg ttcgcagtgt ggcgcacgct
      781 atgagcgccc gctgggaatt ctgggtcgac cgcggcggca ccttcaccga catcgtcgcg
      841 cgccgcccgg acggcagcct gctcacccac aagctgttgt ccgaggatcc gcgctatgcc
      901 gatgcggcgg tcgccggcat tcgccacatc ctcggcctga gctccggcga acccatcccg
      961 gcccaactga tcgagtcggt gaagatgggc accacggtcg caacgaatgc gctgctcgaa
     1021 cgcaagggcg acccgacctt gctggtgatc acgaaaggct tccgcgacgc gctgcgcatc
     1081 ggctaccaga accgcccgcg cctgttcgac cgccatgtcg tgctgcccga actgctgtac
     1141 cgcgacgtga tcgaggccga tgagcgcatc gatgcgcatg gaggcgtcgt cgccgcactc
     1201 gataccgaac gggtgcggca ggatctgcag cagcgccata cggacggcta ccgggcggtg
     1261 gcgatcgtgc tgatgcacgg ctatcgctat cccgagcatg aacgggcgat tgccgcgatc
     1321 gcccgcgatc tcggcttcac acaagtttcg gtgtccagcg aagtcagtcc gctgatgaag
     1381 ctggtctcgc gcggcgacac cacggttgtc gacgcctatc tgtcccctat cctgcgccgc
     1441 tacgtggacc aggtggcatc ggaactgcgc ggcgtgcgcc tgctgttcat gcagagcaac
     1501 ggcgggctta ccgatgcgcg ccgcttccag ggcaaggatg cgatcctctc cggaccggcc
     1561 ggcggcatcg tcggcatggc gcgcaccgcc gaaagcgccg gtttcgatcg catcatcggt
     1621 ttcgacatgg gcggcacctc aaccgacgtc tcccactatg ccggcgaatt cgagcgcgag
     1681 ttcgacaccc aggtcgccgg agtgcgtctg cgcgcaccga tgatgagcat ccataccgtc
     1741 gcagcgggcg gcggctccgt gctgcacttc gacggtacac gttttcgcgt cggcccggac
     1801 agcgcgggcg caagtccggg accggcctgc taccggcgcg gcggcccgct cgcggtcacc
     1861 gactgcaatg tcatgctcgg caagatccag ccgaaccatt ttccggcggt gttcggcgac
     1921 atgggcaacc agacgctcga ccgcgaaatc gtcgcgcaga aattttccgc gctggcgcag
     1981 gacatcttcc gcgccaccgg ccagcgccgc acgcccgagg aagtggccga gggcttcatc
     2041 gaggtcgcgg tcggcaacat ggccaatgcg atcaagttca tctccgtaca gcgcggtcac
     2101 gacgtgaccg gctacacgct ctgcaccttc ggcggcgccg gcggccagca cgcctgcctg
     2161 gtcgcggacg cgctcggcat gacgcgggtg ttcgcgcatc cgctggccgg cgtgctgtcc
     2221 gcctatggga tgggcctggc agaccagacc gcgatgcgcg aacaggcgct ggaagcgccg
     2281 ctcggcgagg cggcaatgcc ggccgcgcag gcgcacctga acgcgctggc cgaagccgcc
     2341 cgcgccgatc tgctcgccca gggcgtggag gcggcgcgca tccgcgtggt ggagcgcgcg
     2401 cacctgcgct acagcggcac cgattcggcc atcgtcgtgc cgttcggctc cgtcgccgac
     2461 atgaccgcgc gcttcgaggc ggcctaccag cggcgctatt ccttcctgat gcaggacaag
     2521 ggcctggtga tcgaggcgct gtcggtcgag gccatcggcg tggccgacga cgtggcggcg
     2581 catgccgaag tgctgcccga gcgcgtgggg cggctcgggc cggccgcgac ggtcaggatg
     2641 ttctccggcg gcagatggca cgacgcgccg ctctacctgc gcgccgacat ccgccccggc
     2701 gaccacattg cgggcccggc catcatcgcc gagaagaatg ccaccaccgt ggtcgagccg
     2761 ggctgggatg ccgaggtcac gccgctgaac cacctcgtgc tcgaacgcgt cgaggcgcgt
     2821 ccggcgcgca aggcgatcgg cacatcggcc gatccggtga tgctcgaagt attcaacaac
     2881 ctgttcatga gcattgccga gcagatgggc ctgcgcctgc agaacaccgc gttctcggtc
     2941 aacatcaagg aaaggctgga cttctcctgc gcgctgttcg acgcacacgg caacctgatc
     3001 gcgaacgcgc cgcacatccc ggtgcatctc ggctcgatgg gcgagagcat caagacggtg
     3061 atccgcgaga acggcgcgac catgcgcgcg ggcgacgtgt tcatgctcaa cgacccgtac
     3121 cacggcggca cccacctgcc tgacgtgacc gtgatcaccc cggtcttcga ccgggcgggc
     3181 gcgaacgtgc tgttccatgt cgcctcgcgc ggccaccatg ccgacatcgg cgggaccacg
     3241 cccggctcga tgccgcccga ctccaccata gtcgaggagg aaggcgtcct gatcgacaac
     3301 ttccagctgg tccgcgcggg ccgcctcctc gagcaggaaa ccgtcgccct gctgtcctcc
     3361 ggtcgctacc cggcacgcaa catccggcag aacctcgcgg acctgcaggc acagatcgcc
     3421 gccaaccaga agggcgcgga tgaactgctg aagatggtcg accacttcgg tctcgaggtg
     3481 gtgcacgcct acatgcggca cgtgcaagac aacgccgagg aagcggtgcg gcgcgtgatc
     3541 agcgcattga aggacgggcg ctacacctac cggctcgaca acggcgcggt gatcgaggtc
     3601 gcgatccgcg tcgaccgcgc cgcgcgcagc gccgacatcg atttcaccgg cacctcgccg
     3661 cagctgcaaa acaacttcaa cgcgccgtcc gccgtgtgca tggctgcggt gctgtacgtg
     3721 ttccgcacgc tggtcgacga cgacatcccg ctcaacgccg gctgcctgaa gcccttgaac
     3781 gtgatcatcc ctgaaggctc gatgctcaat ccgcactatc cggcggcagt ggtctcgggc
     3841 aacgtggaaa cctcgtcctg cgtgaccaac gcgttgtacg gcgcgcttgg cgtgatggcg
     3901 agtgcgccgg gcacgatgaa caacttcacc ttcggcaacg aacgccacca gtactacgaa
     3961 acgatatccg gcggttccgg agccggggac ggcttcgacg gcaccgacgt ggtccagacc
     4021 cacatgacca attcacgtct gaccgatccc gaagtgctgg aatggcgctt tccggtacgg
     4081 ctggagagct acgagatccg ccatggctcg ggcggggccg gacgctggcg tggcggcaac
     4141 ggcggcgtgc gcaggattcg cttcctcgaa cgcatgaccg cgtccatact ctccaacaac
     4201 cgcgtcgtgc cgcccttcgg cgcggccggc ggcggcgacg gccagtgcgg gcgcaactac
     4261 gtgatccgcg tcgacggccg ggtcgaaacg ctcggcccga ccgcacgcac cgaactggaa
     4321 cccgacgacg tgttcgtgat cgagaccccc gggggtggcg gatttggcca agcgcaagac
     4381 tgatcaatcc cgtcagtgca gggtggtaca gtaggaaacg tccggcgcgc cgcggcggca
     4441 cgaccagaaa gaggagcaag gagacatgag cgacaagatc gaactcgccc ggcgcgtcgg
     4501 gcaggcgctg caggaacgcg gctggctgct ggcggtggcc gaatcgtgca ccggcggcgg
     4561 cgtcggacag gcggtcacgg agatcgcggg gtcatccgaa tggttcgact gcggtttcat
     4621 cgtctattcg aacgcgtcga agaccgaact ggtggacgtg ccggcggcga tgatcgccca
     4681 gtacggcagt gtcagcgagg aagtggccgg ggcgatggcc gaaggcgcgc tcgccaacag
     4741 caacgcccat gtcgcgctgt cgaccactgg catcgcggga ccggccggcg cggtgcccgg
     4801 caagccggtc ggcacggtct gcttcggctg ggccgccgag caccgtacct ataccgaacg
     4861 ccacgtgtat tccggtgacc gccaatctgt gcgcgagcag actatcgtcc acgcgctcga
     4921 agggctgctg cgcttcctcg agcagaagtg aaccggtcta gcgataccgg ttgatcgcgt
     4981 cgcgcgtctc gatcgcgacg cggcgcgcgg cgtcggtgaa gcgctcgccg gcttctgcct
     5041 tcgcgtacag gatcgcgcgc gacgaattga tcatcatgcc cagccccgcc gtggtgcgtc
     5101 cggccttcac ggtcgcttcc acatcgccgc cctgggcgcc gacgcccggc accagcagcg
     5161 gcatatcgcc gacgatcctg cgcacctcgg ccaactcctg tgggtaggtc gcgccgacca
     5221 caaggccgca ctgcccgttc gtattccatt tctgcgccac caggcgcgcg acatgctggt
     5281 acaccggcat cccgtcggcc gtcaggaact gcaggtccga gccgcccggg ttcgacgtgc
     5341 ggcacagcac gatcacgccg cgatccttcc attccaggta aggctgcacg gaatcgaagc
     5401 ccatgtaggg attgaccgtg accgcatgcg cgccatagcg ctcgaaggcc tcgcgcgcat
     5461 actgctcggc ggtggcgccg atatcgccgc gcttcgcatc gaggacgata gggatatgcg
     5521 ggtgggcgct gcgcaggtgc gcgcacagcg cctcgagctg gtcttcggcg cgcaaggcgg
     5581 cgaagtaggc gatctgcggc ttgaacgcgc aggcaacatc cgccgttgcg tcggcgatcg
     5641 ccttgcagaa ctcgaacacc gcttgcgggc ggcctgccag ctcggcggga aagcgggaga
     5701 cgtcgggatc gaggccaacg cacagcagcg agtcgttgcg cgcccaggcg gcggagagtt
     5761 gttcggtgaa agtcacggcg gggaatcggg gaagttgcct ggcgaaatgc gcattgtacc
     5821 gcgccatgct atcgttcctg ccctgtcttc ccg
//
LOCUS       DATLGF010000434        10152 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_215435, whole genome shotgun sequence.
ACCESSION   DATLGF010000434 DATLGF010000000
VERSION     DATLGF010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 10152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10152
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215435"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..1958
                     /locus_tag="VM406_13215"
     CDS             285..1958
                     /locus_tag="VM406_13215"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter family protein"
                     /protein_id="HVL76968.1"
                     /translation="MTNVYYQFALLLLAATVVGALALRLRQPLIVAFIIVGIGLGPSG
                     LDLVTAHEEVNLLAQIGVTVLLFVVGLKLDLHMVRNVGPVALATGLGQLLFTIVIGFG
                     ITIALGMPALTALYVAVALTFSSTIIIVKLLSDKREVDALHGRIAVGFLIVQDIAVVV
                     AMLLLGSAQAGEGTDASALWILALTVALKIAAAIALVALLMRYVLPRLLRLLAQSQDL
                     LLIFAVAWGTVLAASGDMFGFSKEVGAFLAGFSLASTPYREAIASRLSGLRDFLLLFF
                     FIDMGSHLDLRILGDQMGAAIILSLFVLIGNPLIVMAIMGYMGYRKRTGFLAGLTVAQ
                     ISEFSIVFVAMGISLGHIAQQALGLVTLVGLVTITLSTYLILYSYPLYTRLAPYLGWF
                     ERKEPFREQAIERQHRDETPVDVIVFGAGRFGRRLLHQLRARGYSVLGVDFDPDAVQS
                     LRADGIPVHYGDAEDAELASTLPLAGARWVVSTLAEADANRLLMQVVRDNGFKGKTAI
                     ALRSPHHEEFPSGLVVDKVFRPFDDAADFAMEEIAQSLGPDTTSQQRKG"
     gene            1961..2842
                     /locus_tag="VM406_13220"
     CDS             1961..2842
                     /locus_tag="VM406_13220"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVL76969.1"
                     /translation="MELRNILAATDFSDNAQRAVERAALLARQHGATLHLAHVIPTIS
                     WTMFGRALVEHPLITEKHFYDAAHDRLERVAQDCRTRFGVQVDCHVEIGRADEKISAL
                     ASRYPVDLTVLGPHDEHFPRNLFVGSTARRYLHHTRHAALVSRSEAASPYARLLVAVD
                     FSDTARRALQAAVAIAPDAEIHAMHVYELLFEGKMRYAGVEQNVIDQYRDAAAAEARQ
                     QMHAFLQELGLGTRVQTTVQGGYPAQALLEQAQRIGADLLVMGRRGRSELDELFLGSV
                     TENVLLDLDRDLLLVST"
     gene            2860..3387
                     /locus_tag="VM406_13225"
     CDS             2860..3387
                     /locus_tag="VM406_13225"
                     /inference="COORDINATES: protein motif:HMM:NF014012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HVL76970.1"
                     /translation="MSRPAPLHAATGTRPVFGALAWRTLVFAFLWWVLVGGYLGSWYV
                     GVPSIALAVWSSMKLSPPPALRFSPLALLTFFIFFIVQSLKGGVQVAVMALRPTLDLH
                     PAQLDLPLRVSGPAQLVLASAVSLMPGTLAMTVENNRLRLHVLDARLPIARDVRAAER
                     RIARIYGQELAEGDE"
     gene            3384..3695
                     /locus_tag="VM406_13230"
     CDS             3384..3695
                     /locus_tag="VM406_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL76971.1"
                     /translation="MNVLLAIFLLANLLVALVRVVRGPTAADRLLVGLLFGTTGAAIL
                     VLLAGEGVSGGLVDTALVFALLAAITGAAFAQRAWRRDVDEASLVDEEPEDNSGRSDD
                     A"
     gene            3688..3990
                     /gene="mnhG"
                     /locus_tag="VM406_13235"
     CDS             3688..3990
                     /gene="mnhG"
                     /locus_tag="VM406_13235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HVL76972.1"
                     /translation="MLEWLSLALLLVGAAFFFAGTVGLLRFPDVYTRLHVLAKVDNLG
                     LGFVLLGLLLRANSIAGALKLVLVWLLVLAASAVISFLIARRASRRGIAPWTAGGP"
     gene            4013..4291
                     /locus_tag="VM406_13240"
     CDS             4013..4291
                     /locus_tag="VM406_13240"
                     /inference="COORDINATES: protein motif:HMM:NF024641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HVL76973.1"
                     /translation="MQTATLAFDLLLSAALIWSAIRAITVADLFRAVVMFIVFGLLVA
                     LVWARLDAPDIALAEAAIGAGLTGALLLDAVGHLRAIQLVREKKEGRR"
     gene            4297..4971
                     /locus_tag="VM406_13245"
     CDS             4297..4971
                     /locus_tag="VM406_13245"
                     /inference="COORDINATES: protein motif:HMM:NF015970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit B"
                     /protein_id="HVL76974.1"
                     /translation="MRALLVILLCSAFGAALVYAILQLPPQAVDLPQQVSANIDESGV
                     AHPVTAVLLNFRGYDTMLEVAVLLLALLGVLAGTSVEKRRRAAGERPTLPLLRGFTRL
                     IVPLMVLVAGYLLWAGSSMPGGAFQAGAVVAGAGVLLHLARLLPAWATPNLMQRWGLA
                     IGFLVFLAFAALPALQDGSTMLQYPLAWAGALILLIEAGLTLSLGLLLAGLFLCLSDD
                     GMEFEK"
     gene            4968..5285
                     /locus_tag="VM406_13250"
     CDS             4968..5285
                     /locus_tag="VM406_13250"
                     /inference="COORDINATES: protein motif:HMM:NF012636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HVL76975.1"
                     /translation="MTLTLTPSLVYALTGALLFAIGVAGLIVRTHLLRKILAFNVMGS
                     GAFMVLVGLGQRAGISDPVPQAMVLTGIVVAVAATALALALSRHLLLLTGNMHLPEND
                     PDA"
     gene            5278..6732
                     /locus_tag="VM406_13255"
     CDS             5278..6732
                     /locus_tag="VM406_13255"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HVL76976.1"
                     /translation="MHDLSLHAIAWLILLPLAWASAAFLLGPGRGAGLAVIGLALQLL
                     LAISLAVGVVDQGPLAYQVGGWGAPLGIDLLADGLSSVMLLLTQVVALPLALYAHAYF
                     SQNPKGVTWFWPLVGYLVAAMNALFLSADAFNIYVTLELVGLAAVGLVAVAGGVGPVT
                     AALRYLLTTLLGSGAYLLGVALLYGTYGTVSLEVLGPLIAEQAPLAVAMAGALMLFGM
                     LLKTALFPFHFWLPPAHGGAPAPVSAILSALVIKASFYLVVRFWLSIFEPLTDYAVAQ
                     LLGLFGAGAVLWGSLQALQQTRLKMLVAYSTVAQVGYLFLLFPLVTGTPEAAANSAVQ
                     GVIMQALAHGLAKAAMFTAAGAMVLSMERDDISRLGGLSSHMPQSLFAFGLAGVTLMG
                     LPPSAGFLAKWLLIDAAIHSGQWGWIVMLIAGGLLTAAYVFKVLRQAFLQVEQDAHYR
                     PLPRMLEWVPLVLALASLALGVRASEIILLTGVR"
     gene            6729..8231
                     /locus_tag="VM406_13260"
     CDS             6729..8231
                     /locus_tag="VM406_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HVL76977.1"
                     /translation="MSFNESLPLLTLFSSLLPGLVIFGLREERVALRTTLNIVGAVAK
                     LALIGYMLWGVAQGEEYVFVHTVLPGIHLVFRADALALLFVTLSAALWMLTTLYAIGY
                     LEDAPHRSRFFGFFSLCVTATVGVAMAGNLFTFFFFYELLTLSTYPLVVHRGTDASVR
                     GGSIYLGYTLFGGAVLLTGVVWLYGLLGQVDFIQGGAASLLGPEHTRQLQFVFLLLII
                     GVGVKAAIVPFHGWLPQAMVAPAPVSALLHAVAVVKAGAFGVVRIVYEVYGVEFAASL
                     NLLTPLAVAAAITIIWGSLRALFQNDLKRRLAYSTVSQVSYIVLGVALFGPVGTVGGL
                     VHLVHQGIMKITLFFCAGNYAETLGVHKVSELNGVGRRMPLTTIAFSIGALGMIGAPL
                     TAGYVSKTYLTDGAIAAQMDWAIWVLNISALLNAAYFLPILFRAWVLRQREPWHAEHI
                     PARGWRETNWLLLLPPLISALATVLAAVMADAAWSPLTWARFISAREYLQ"
     gene            complement(8237..8998)
                     /locus_tag="VM406_13265"
     CDS             complement(8237..8998)
                     /locus_tag="VM406_13265"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVL76978.1"
                     /translation="MLPTLLLWLGQINILLALFNLVPGFPLDGGRILRAVLWGITGNL
                     RRATGWATGAGQLFAWFLIGSGLAMMLGFRIPVFGTGFIGGLWIAFIGWFLNNAALLS
                     YQQLVVRESLEDVPVAALMQTRFTDVPPDLSVDRLVEDLILHSEQRGFPVLSGERLAG
                     MVCMEDVRKLERSMWPFKRVGDIMTPARSLICIGPQEPAYRALALLGDGRINQMPVVE
                     NGRVRGLIRREDILKWLSLRGKFDSRDRIAYGEGR"
     gene            complement(9028..9480)
                     /locus_tag="VM406_13270"
                     /pseudo
     CDS             complement(9028..9480)
                     /locus_tag="VM406_13270"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
     gene            complement(9493..>10152)
                     /locus_tag="VM406_13275"
     CDS             complement(9493..>10152)
                     /locus_tag="VM406_13275"
                     /inference="COORDINATES: protein motif:HMM:NF006072.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL76979.1"
                     /translation="VADLTADKLRETVRLCNESGSDTRGYALNVTDEAAVEDAFCRVA
                     QDFGALDGLINNAGVNADALLVKRDGGEIGKMSLADFNKVLQVNLVGVFLCGREAAVH
                     MLALGRGGVLINISSIAQVGNIGQSNYAAAKAGVSAMTVTWAQELAQYGIRVAGIAPG
                     FTSTPMVARMKPAMQDRFRQRIPLRRFAEPVEIAHAAAFILENDYFSGRILEIDGGLR
                     L"
BASE COUNT         1437 a         3515 c         3314 g         1886 t
ORIGIN      
        1 ggcctcggcg atcagcgttc gccgtagccg ccgcaagccg gcgacgcaat gcacaagccc
       61 gatgccgtga tacggcatcg ggctttttct ttggggccgc ggttcggtcg ctgcgatctg
      121 atgccgttca caaagacgat cgctcatctg aacggcatac cccacttcca ccctgttttt
      181 gacctaggtc atgggcatta ccttcctgca acgctactgt ccggcgtccc ggaagaaagc
      241 ggagcgcccc cagcacgact gcgcgcgcgc cgcatccaag ccacatgacc aacgtctact
      301 atcaattcgc cctcctcctt ctggccgcta cggtggtagg cgcgcttgcg ctgcgtctgc
      361 gccagccgct gatcgtggcc ttcatcatcg tcggcatcgg cctgggcccg tccgggcttg
      421 accttgtcac cgcgcacgaa gaagtcaacc tgctcgcgca gatcggcgtg accgtgctgc
      481 tgttcgtggt cggactcaag ctcgacctcc acatggtgcg caacgtcggc cctgtcgcac
      541 tcgccaccgg cctcggccag ctgctgttta ccatcgtcat cggctttggc atcacgatcg
      601 ccctcggcat gccggcgctg accgccttgt atgtcgcggt cgccctcacc ttctccagca
      661 cgatcatcat cgtcaagctc ctgtcggaca agcgggaggt cgacgcgctg cacgggcgca
      721 tcgccgtggg cttcctgatc gtgcaggaca tcgcggtggt cgtggcgatg ctgctgctcg
      781 gctcggcaca ggccggcgaa ggcaccgacg cctcggcgtt gtggattctg gccctcacgg
      841 tcgcgctgaa gatcgccgcc gcgatcgcgc tggttgcgct gctgatgcgc tacgtgctgc
      901 cacggttgct gcgcctgctc gcgcagtcgc aggacctgct gctgatcttc gcggttgcct
      961 ggggaacggt gctcgcggcc tccggcgaca tgtttggctt ctccaaggag gtgggcgcct
     1021 tcctggccgg tttctcgctc gcttcgacgc cttatcgcga ggcaatcgcc tcgcgcctgt
     1081 ccggcctgcg cgacttcctg ctgcttttct tcttcatcga catgggcagc cacctggacc
     1141 tgcgcatcct gggcgaccag atgggcgcgg cgatcatcct ttcgctcttc gtgctgatcg
     1201 gcaatccgct gatcgtgatg gccatcatgg gctacatggg ctaccgcaag cgcaccggct
     1261 ttctcgccgg gctcacggtc gcgcagatca gcgagttctc gatcgtcttc gtcgccatgg
     1321 gcatctcgct cggccacatc gcccagcagg cgctcggact ggtgacgctg gtggggctgg
     1381 tgacgatcac cctctccacc tacctgatcc tctactcgta tccgctgtac acccggctcg
     1441 ccccctatct cggctggttc gagcgcaagg agccgttccg cgagcaggcg atcgagcgcc
     1501 agcaccggga cgagacgccg gtcgatgtga tcgtgttcgg cgccggccgc ttcggccgac
     1561 gcctgctgca ccagctgcgc gcgcgcggct acagcgtgct gggcgtcgac ttcgatccgg
     1621 acgccgtcca gtcgctgcgc gccgacggca tcccggtgca ctacggcgac gccgaggatg
     1681 ccgagctcgc ctccaccttg ccgctggccg gcgcgcgctg ggtggtcagc acgcttgccg
     1741 aggccgatgc aaaccgtctg ctgatgcagg tcgtgcgcga caacggcttc aagggcaaga
     1801 cggcgatcgc gctgcgcagc ccccaccacg aggaatttcc gagcggcctc gtggtcgaca
     1861 aggtattccg gccgttcgat gacgcggccg actttgccat ggaggaaatc gcccagtcgc
     1921 tcggacctga caccacaagc caacaaagga agggatgacc atggaactca ggaacatact
     1981 tgccgccacc gacttctcgg acaacgcaca gcgtgccgtc gagcgcgccg cgctgctggc
     2041 gcgccagcac ggtgccacgc tgcacctggc gcacgtcatc ccgaccatat cgtggacgat
     2101 gttcggccgt gcgctggtcg aacatccgct cattaccgag aagcacttct atgacgctgc
     2161 gcacgaccgc ctcgaacgcg tggcgcagga ctgccgcacg cgcttcggcg tgcaggtcga
     2221 ctgtcatgtc gagatcggac gcgccgatga aaagataagc gcgcttgcgt cccgctaccc
     2281 ggttgacctg accgtgctcg gcccccacga cgagcatttc ccgcgcaatc tgttcgttgg
     2341 ctccactgcc cgccgttacc tgcaccacac gcgacacgcg gcattggtct cgcgatcgga
     2401 ggccgcgtcg ccttacgcgc gcctgcttgt cgcggttgat ttttccgata ccgcgcggcg
     2461 cgcgttgcaa gccgcggtgg cgatcgcacc ggacgcggag atccacgcga tgcacgtgta
     2521 cgaacttctg ttcgaaggca agatgcgcta tgccggagtc gagcagaatg tcatcgatca
     2581 gtatcgcgac gccgcagcgg cggaagcacg gcagcagatg catgccttcc tgcaggaact
     2641 cggcctcggc acgcgcgtgc agacgacggt ccagggcggt tatcccgcgc aggcgttgct
     2701 ggaacaagcg cagcgcatcg gcgcggacct gttggtgatg ggacggcgcg gccgctccga
     2761 actggacgag ctctttctcg gcagcgtcac cgaaaacgtg ctgctcgacc tcgatcgcga
     2821 ccttttgctt gtatccacct gacaggagtc acaaccgaca tgtccaggcc tgcgcccctc
     2881 catgcggcaa ccggcacgcg ccccgtgttt ggcgcgctgg cgtggcgcac gctggtcttc
     2941 gcctttctct ggtgggtgct ggtcggcggt tacctcggca gctggtatgt cggcgtgcca
     3001 agcatcgctc tggcggtctg gagcagcatg aagctgtcgc cgccgccggc gctgcgcttc
     3061 tcgccgctgg cgctgctgac gttcttcata tttttcatcg tccagtcgct caagggcggc
     3121 gtgcaggtgg cggtcatggc gctgcgtccg acgctcgacc tgcaccccgc ccagctcgac
     3181 ctgccgttgc gcgtgagcgg cccggcccag ctggtactgg ccagcgcggt cagcctgatg
     3241 ccgggcacgc tggccatgac ggtggaaaac aaccggctgc gcctgcacgt gctcgatgcc
     3301 aggctgccga ttgcgcgcga cgtgcgcgcc gcggagcggc gcatcgcgcg catctacggg
     3361 caggaacttg cggagggcga cgaatgaacg tgctgctcgc catcttcctg ctcgccaacc
     3421 tgctggtggc gctggtgcgc gtggtacgcg gcccgaccgc ggcggaccgc ctgctcgtgg
     3481 gactgctgtt cggcacaacc ggcgccgcca tcctggttct gctcgccggt gaaggcgtct
     3541 ccggcggact ggtcgacacc gcactcgtgt tcgcgctgct tgcggcgatc accggagccg
     3601 cgttcgcaca gcgggcctgg cggcgggatg tcgatgaagc gagcctggtg gacgaggagc
     3661 ccgaggacaa cagcgggagg agcgatgatg cttgaatggc tcagccttgc cctgctcctg
     3721 gtcggcgccg ccttcttttt cgccggcacc gtcgggctgt tgcggtttcc ggacgtgtac
     3781 acgcggctgc acgtgctcgc gaaggtcgac aacctgggac tcggcttcgt gctgctcggc
     3841 ctgctgctgc gcgccaactc cattgccggc gcgctcaagc tggttctggt ctggctgctg
     3901 gtcctggccg cgagcgctgt gatcagcttc ctgatcgcgc gccgcgcgag ccggcgcggc
     3961 atcgccccgt ggaccgcggg cggcccctga tctcttcccg gagaacaaga acatgcaaac
     4021 cgccaccctc gcgttcgacc ttttgctctc cgccgcgctg atatggagcg cgatccgcgc
     4081 catcaccgtc gccgacctgt tccgcgcggt cgtgatgttc atcgtgttcg gtctgttggt
     4141 cgcgctggtc tgggcccgcc tggacgcccc cgatatcgcg ctggcagaag cggcgatcgg
     4201 cgccggcctg accggcgccc tgctgctcga cgcggtcggc cacctgcggg cgatccagct
     4261 cgtgcgcgag aagaaggagg gacgccggtg accgcaatgc gcgccctgct ggtgatcctc
     4321 ctctgcagcg cgttcggcgc cgccctcgtc tacgcgatcc tgcagctgcc gccgcaggcg
     4381 gtggacctgc cgcagcaggt gtcggccaat atcgacgaat cgggcgtcgc gcacccggtg
     4441 actgcggtgc tgctcaattt ccgcggctac gacacgatgc tcgaggtcgc cgtgctgctg
     4501 ctcgccctgc tcggcgtgct cgccggcacc agcgtcgaga aacgccgccg ggctgccggc
     4561 gaacgtccga cgctgccgct cctgcgcggt ttcacccgac tgatcgtgcc actgatggtg
     4621 ctggttgcag gctatctgct gtgggcggga tcgagcatgc cgggcggcgc gttccaagcc
     4681 ggcgcggtgg tcgccggcgc cggcgtgctg ctgcacctgg cgcgcctgct cccggcctgg
     4741 gccacgccga acctcatgca gcgctggggc ctggcgatcg gctttctcgt cttcctcgcg
     4801 ttcgccgcgc tacccgcgct gcaggacggc agcacgatgc tgcagtaccc gcttgcctgg
     4861 gccggcgcgc tgatcctgct gatcgaagcc gggctgacgc tctcgctcgg cctgctgctg
     4921 gccggcctgt tcctgtgtct gtccgacgat ggcatggagt tcgagaaatg accctcaccc
     4981 tcacgccttc gctcgtgtat gcgttgacgg gcgcactgct gttcgcgatc ggcgtggccg
     5041 gcctgatcgt gcgcacccac ctgttgcgca agatcctcgc gttcaatgtg atgggcagcg
     5101 gcgccttcat ggtgctggtc ggtcttggcc agcgcgcggg catatcggat ccggtgccgc
     5161 aggcgatggt gctgaccggc atcgtggttg cggtcgccgc cacggcgctt gcgcttgcgc
     5221 tgtcgcgcca cctgcttctg ctgacgggca acatgcacct gccggagaac gatccggatg
     5281 catgacctgt ccctccacgc gatcgcctgg ctgatcctgc tcccccttgc ctgggcgagc
     5341 gcggccttcc tgctcggccc cgggcgcggc gccgggctcg cggtcatcgg actggcgctg
     5401 cagctgctgc tggcaatcag cctggcggtc ggcgttgtcg accagggccc gctcgcctac
     5461 caggtcggtg gttggggcgc gccgctcggc atcgacctgc tggccgacgg cctgtcttcg
     5521 gtgatgctgc tgctcaccca ggtggtcgcg ctgccgctgg ccctgtatgc gcacgcctat
     5581 ttctcgcaga atccgaaggg tgtcacctgg ttctggccgc tggtcggcta cctggtcgcg
     5641 gcgatgaacg cactgttcct gtcggccgac gcgttcaaca tctacgtgac gctggagctg
     5701 gtcgggctgg ccgcggtcgg cctggtcgcg gtggccggcg gcgtgggccc ggttaccgcg
     5761 gccctgcgct acctgctgac caccctgctt ggctccggcg cctatctgct cggcgtggcc
     5821 ctgctgtacg gcacctacgg cacggtttcc ctcgaggtgc ttggtccgct gatcgcggaa
     5881 caggcgccgc tggcagtcgc catggccggc gcgctgatgc tgttcggcat gctgctcaag
     5941 accgcgctgt tcccgttcca cttctggctg ccgcccgcgc acggcggcgc gcccgcgccc
     6001 gtgtcggcca tcctgtccgc gctggtcatc aaggcctcgt tctacctggt cgtgcgcttc
     6061 tggctgagca tcttcgagcc gctgaccgac tacgcggtcg cgcagctgct cggcctgttc
     6121 ggcgccggtg ccgtgctgtg gggctcgttg caggcgctgc agcagacgcg cctgaagatg
     6181 ctggtggcct attcgacggt cgcgcaggtc ggctacctgt tcctgctgtt cccgctggtg
     6241 accggcacgc ccgaggccgc cgccaacagc gccgtgcagg gcgtgatcat gcaggcgctg
     6301 gcccacggcc tggcgaaagc ggcgatgttt accgcggccg gcgcgatggt gctgtcgatg
     6361 gagcgcgacg acatcagccg cctgggcggg ctgtccagcc acatgccgca gtcgctgttc
     6421 gcgttcgggc tggcgggcgt gacgctgatg ggcctgccgc cctcggccgg cttcctcgcg
     6481 aagtggctgc tgatcgatgc cgcgatccac agcggtcagt ggggctggat cgtgatgctg
     6541 attgccggcg ggctgctgac cgccgcctat gtgttcaagg tgctgcgcca ggccttcctg
     6601 caggtcgagc aggacgcgca ctaccggccg ctgccgcgca tgctcgaatg ggtgccgctg
     6661 gtgctggcgc tggcgagcct cgcgctgggt gtacgcgcca gtgaaatcat cttgctgacc
     6721 ggggtgcgat gagcttcaac gaatcccttc cgctcctgac cctgttctcc tccttgctgc
     6781 cggggctggt catcttcggg ctgcgcgagg agcgcgtggc gctgcgcacc acgctcaaca
     6841 tcgtcggcgc cgtcgccaag ctcgcgctga tcggatacat gctgtggggc gtggcgcagg
     6901 gggaggagta cgtgttcgtg cacacggtgc tgcccggcat ccacctggtc ttccgcgccg
     6961 acgcgctcgc actcctgttc gtcacgttgt cggcggcact gtggatgctg actacgctgt
     7021 atgcgatcgg ctacctcgaa gacgccccgc accgcagccg cttcttcggc ttcttcagcc
     7081 tgtgcgtcac tgccaccgtc ggcgtggcga tggccggcaa cctgttcacc ttctttttct
     7141 tctacgagct gctgacactg tcgacctatc cgctggtcgt gcaccgcggc accgacgcct
     7201 cggtgcgcgg cggcagcatc tacctcggct acacgctttt cggcggcgcc gtgctgctga
     7261 ccggcgtggt ctggctgtac ggcctgctcg gccaggtcga cttcatccag ggcggtgcgg
     7321 cctcgctgct tggccccgag cacacgcggc agctgcagtt cgtgttcctg ctgctgatca
     7381 tcggggtcgg cgtgaaggcg gcgatcgtac ccttccatgg ctggctgccg caggcgatgg
     7441 tggcgcctgc gccggtctcg gcactgctgc atgcggtggc ggtcgtgaaa gccggcgctt
     7501 tcggcgtggt acgcatcgtc tacgaggtct acggcgtgga gtttgccgcc tcgctgaacc
     7561 tgctcacgcc gctggcggtc gccgctgcga tcaccatcat ctggggctcg ctgcgcgcgc
     7621 tgttccagaa cgacctgaag cggcgtctcg cgtactcgac ggtcagccag gtgtcatata
     7681 tcgtgctggg cgtggcgctg ttcggcccgg tgggaaccgt aggcggcctc gtgcacctgg
     7741 tccaccaggg catcatgaag atcacgctgt tcttctgcgc gggaaattac gccgagacgc
     7801 tgggcgtgca caaggtcagc gagctcaatg gcgtgggccg gcggatgccg ctgaccacca
     7861 tcgcgttctc gatcggcgcg ctcgggatga tcggggcgcc gctgacggcc ggctacgtca
     7921 gcaagaccta cctgaccgac ggcgcgatcg ccgcgcagat ggactgggcg atctgggtgc
     7981 tcaacatctc ggccctgctc aacgccgcgt atttcctgcc catcctgttc cgtgcctggg
     8041 tgctgcgcca gcgcgaaccc tggcatgccg aacacatccc cgcgcgcggc tggcgcgaga
     8101 cgaactggct gctgctgttg ccgccgctga tcagcgcgct ggcgaccgtg ctggctgcgg
     8161 tgatggccga cgcggcctgg agcccgctta cctgggcacg attcatctcg gcgcgcgaat
     8221 acctgcaata gcagattcag cgcccttcac cgtaggcaat gcggtcgcgc gaatcgaact
     8281 tgccgcgcaa cgacagccac ttgaggatgt cctcgcgccg gatcagcccg cgcacccgtc
     8341 cgttctcgac gaccggcatc tggttgatcc tgccgtcgcc caggagcgcc agcgcgcggt
     8401 aggccggctc ctgcgggccg atgcagatca gcgagcgcgc cggcgtcatg atgtcgccca
     8461 cgcgcttgaa cggccacatg ctgcgttcga gcttgcgcac gtcctccatg caaaccatgc
     8521 cggccagccg ctcgcccgac agcaccggaa agccgcgctg ctcgctgtgc aggatcaggt
     8581 cctcgaccag cctgtccacc gacaggtcgg gcggcacatc ggtaaaccgg gtctgcatca
     8641 gcgcagcgac gggcacgtct tcgagcgatt cccgcaccac cagctgctgg tagctgagca
     8701 aggcggcgtt gttgaggaac cagccgatga aggcgatcca caagccgccg atgaagccgg
     8761 tcccgaagac cgggatgcga aagcccagca tcatcgccag cccgctgccg atcaggaacc
     8821 aggcgaacag ctgccccgcc ccggtcgccc agcccgttgc gcggcgcagg ttgccggtga
     8881 tgccccacag caccgcgcgc aggatacggc cgccgtcgag cggaaacccc ggcaccaggt
     8941 tgaacaatgc cagcaggatg ttgatctgcc cgagccagag cagcagtgtg ggcagcatgc
     9001 cgaggccggc cagcgttgcg cgatgttgtc cggatcgatg ttatccatcc ccgccagatt
     9061 gaccgcgagc agcacgcagg acacgccgat caccaggctc acgatcggcc cggcgatcgc
     9121 catccagaac tcgccgcgcc agctgcgcgg ctcatcctcc atgtgggcca tgccgccgaa
     9181 cacgaacagc gtgatgcgct tcacatcgat cccgtagcgc cggcctacca gcgcgtgtga
     9241 cagctcgtgc agcagcaccg acacgaagaa caggaaggcc gccccgatgg cggtgaccca
     9301 ccactgggca ggcgtccagt ccgggtgcca caaggggaac agcgcgccgc caagcgagat
     9361 ggtgatcagc gtgaagatga tcagcaggct ccagtccaga tgaacctcga tgccggccac
     9421 gcggccgata cgaaaaccat tacccatttc gccacctcgt cgtgtttcgg tgccggccat
     9481 gcaggaaggc cctcacagcc ttagaccccc gtcgatctcg agtatccgtc cggagaagta
     9541 gtcgttctcg aggatgaagg cggcggcgtg cgcgatttcc accggttcgg cgaagcgccg
     9601 cagcggaatg cgctggcgga agcggtcctg catcgccggc ttcatgcgcg cgaccatcgg
     9661 cgtggaggtg aatccgggcg caatgccggc caccctgatc ccgtactggg ccagctcctg
     9721 cgcccatgta accgtcattg ccgacacgcc ggccttggcc gccgcatagt tggactggcc
     9781 gatgttgccg acctgggcga tgctcgaaat attgatgagg acgccgccgc ggcccagcgc
     9841 gagcatgtgc acggcggcct cgcgaccgca caggaacacg ccgaccaggt tcacctgcag
     9901 caccttgttg aagtcggcca ggctcatctt cccgatctcg ccaccgtcgc gcttgaccag
     9961 cagcgcatcg gcgttcacgc cggcattgtt gatcagcccg tcgagcgcgc cgaaatcctg
    10021 ggcgacgcga cagaacgcgt cctcgaccgc ggcctcatcg gtcacattca acgcgtagcc
    10081 gcgcgtatcc gacccgcttt cattgcacag ccgcaccgtt tcccggagct tgtctgccgt
    10141 caggtcggcc ac
//
LOCUS       DATLGF010000435         2902 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_121890, whole genome shotgun sequence.
ACCESSION   DATLGF010000435 DATLGF010000000
VERSION     DATLGF010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121890"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VM406_13280"
     CDS             complement(<1..142)
                     /locus_tag="VM406_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HVL76980.1"
                     /translation="MALATQCPHCRTTFRVAHDQLKLRAGLVRCGACKQIFNGIENLR
                     PPE"
     gene            complement(149..1060)
                     /gene="prmA"
                     /locus_tag="VM406_13285"
     CDS             complement(149..1060)
                     /gene="prmA"
                     /locus_tag="VM406_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HVL76981.1"
                     /translation="MSWTEIVIEVARDHAEALSDALMEAGALSVSVEDADEGTEDEKP
                     LFGEPGMEPAEAAWDRSRVVALAEQDADHASLVAEAAASCGLSGAPAFTLRAVAEQDW
                     VRLTQSQFEPIHIGKRIWVVPSWHEAPDPDGLVLELDPGLAFGTGSHPTTRLCMEWLE
                     EELAAGASVLDYGCGSGILAMVAQKLGADPVHGVDIDPQAIDAARTNSERNHAPVSYH
                     LPEDFSTAVGEMRFDVVVANILSSPLKLMAPMLCARVKAGGSLALSGILARQADEVAA
                     AYAPFIALSVRAERDGWVLLSGQAPQS"
     gene            complement(1071..2444)
                     /gene="accC"
                     /locus_tag="VM406_13290"
     CDS             complement(1071..2444)
                     /gene="accC"
                     /locus_tag="VM406_13290"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HVL76982.1"
                     /translation="MFEKILIANRGEIALRIQRACREMGIKTVVVHSEADRESKYVKL
                     ADESVCIGPAPSAQSYLNMPAIISAAEVTDAEAIHPGYGFLSENADFAERVEKSGFVF
                     IGPRPDSIRMMGDKVSAKQAMIKAGVPCVPGSDGALPEDPKEIIRIARKIGYPVIIKA
                     AGGGGGRGMRVVHTEAALLNAVTMTKSEAGAAFGNPEVYMEKFLENPRHVEIQVLADE
                     FKNAIWLGERDCSMQRRHQKVIEEAPAPGIPRKLIERIGDRCAEACRKIGYRGAGTFE
                     FLYENGEFYFIEMNTRIQVEHPVTEMITGVDLVQEQIRVAAGEKLRYRQRDIELVGHA
                     IECRINAEDPFKFTPSPGRIVAWHPPGGPGIRVDSHAYAGYFVPPTYDSMVGKVIAYG
                     ATREQAIRRMQIALSEMVVEGILTNIPLHRELMVDARFIEGGTNIHYLEQKLADKPDL
                     PKNPKVG"
     gene            complement(2623..>2902)
                     /gene="accB"
                     /locus_tag="VM406_13295"
     CDS             complement(2623..>2902)
                     /gene="accB"
                     /locus_tag="VM406_13295"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HVL76983.1"
                     /translation="APQAAAAAPAPEAPKGHVVKSPMVGTFYRASAPGKPPFVEVGAS
                     VKEGDTLCIIEAMKLLNEIDADASGVIKEILVENGQPVEFGQPLFVIG"
BASE COUNT          465 a          933 c          964 g          540 t
ORIGIN      
        1 gttcgggggg acgcagattc tcgatgccgt tgaagatctg tttgcaggcg ccgcagcgca
       61 ccagcccggc ccgcagcttc agctggtcat gcgcgacccg gaacgtggtc cggcaatgtg
      121 gacattgggt cgcgagtgcc attgcggctc agctctgcgg ggcctgcccg gaaagcagca
      181 cccagccgtc acgctcggcc cggaccgaga gcgcgatgaa gggcgcatag gcggccgcga
      241 cttcatcggc ctggcgcgcg agaatgccgg agagcgccag gctgccgccg gccttgacgc
      301 gggcgcacag catcggcgcc atcagcttga gtgggctgga caggatgttg gcgacgacca
      361 cgtcaaagcg catctcgccg actgcggtgg aaaaatcctc gggcaggtga tagctgaccg
      421 gcgcgtggtt gcgttcgctg ttggtgcgcg cggcgtcgat cgcctgcgga tcgatgtcca
      481 ccccatgcac cggatcggcg ccgagctttt gcgcaaccat tgcgaggatg ccggaaccgc
      541 agccgtagtc gagcacggat gctcccgccg cgagctcttc ctccagccat tccatgcaca
      601 gccgcgtggt gggatggctg ccggtgccga atgccaggcc cgggtcgagt tccagcacga
      661 ggccgtcggg atccggtgcc tcgtgccagc tcggcacgac ccagatacgc ttgccgatgt
      721 ggatgggctc gaactgcgac tgcgtcagcc gcacccagtc ctgctcggcg accgcgcgca
      781 gcgtgaatgc cggcgcgccg gaaagaccgc agcttgccgc cgcctcggcg acgagggagg
      841 cgtggtcggc atcctgttcg gccagcgcaa cgacccggct gcgatcccag gccgcctcgg
      901 ccggttccat gccaggttcg ccgaacagcg gcttctcgtc ttcggtgcct tcgtcggcgt
      961 cttcgacgga taccgacagc gcgccggcct ccatcagcgc gtccgacagc gcttcggcgt
     1021 gatcgcgcgc aacttcgatg acgatttctg tccagctcat ggcgttcagc ttagccgacc
     1081 ttcggattct tcggcaggtc gggcttgtcg gccagcttct gctcgaggta gtggatgttg
     1141 gtgccgcctt cgatgaagcg tgcgtccacc atcagttcgc gatgcagcgg aatgttggtc
     1201 aggatgccct cgaccaccat ctctgacagc gcgatctgca ttcggcgaat cgcctgttcg
     1261 cgagtggcgc cgtaggcgat caccttgccg accatggaat cataggtcgg cggcacgaaa
     1321 tagccggcat acgcatgcga gtcgacgcgg atgccggggc cgcccggagg atgccaggcg
     1381 acgatgcgac ccggcgacgg cgtgaacttg aacgggtctt ccgcgttgat gcggcactcg
     1441 atcgcgtggc cgaccagttc gatgtcgcgc tggcgatagc gcagcttttc gccggccgcc
     1501 acccggatct gttcctgcac caggtccacg ccggtgatca tctcggtcac cgggtgttcg
     1561 acctgaatgc gcgtgttcat ttcaatgaag tagaactcgc cgttttcata caggaactcg
     1621 aaggtgccgg cgccgcggta gccgatcttg cggcaggctt cggcgcagcg gtcgccaata
     1681 cgttcgatca gcttgcgcgg gatgccgggc gcgggtgctt cctcgatcac tttctggtgg
     1741 cggcgctgca tcgagcagtc gcgctcgcca agccagatcg cattcttgaa ctcatcggcg
     1801 agcacctgga tctccacatg gcgcggattc tccaggaatt tttccatgta gacctcggga
     1861 ttgccgaagg cggctcccgc ttccgacttg gtcatcgtga ccgcattgag cagcgcggcc
     1921 tcggtgtgca cgacgcgcat gccgcgtccg ccaccgccgc cggcggcctt gatgatgacc
     1981 ggatagccga tcttgcgtgc aatgcggatg atttccttcg gatcttccgg cagcgcgccg
     2041 tccgatcccg gcacgcaggg cacgccggcc ttgatcatcg cctgcttcgc cgagaccttg
     2101 tcgcccatca tgcgaatcga atccgggcgc ggcccgatga acacgaaacc ggatttctcg
     2161 acccgttcgg cgaagtcagc gttttcggac aggaagccgt agccaggatg gatcgcctca
     2221 gcgtccgtca cttcggccgc gctgatgatc gccggcatgt tcagatagct ctgggccgac
     2281 ggcgccggcc cgatgcagac cgattcgtcg gccagcttca catacttgga ttcgcggtcg
     2341 gcctccgagt gcacgaccac ggtcttgatg cccatttcac ggcaggcgcg ctggatacga
     2401 agggcgattt cgccgcgatt ggcaataagg atcttttcaa acatgggtgc tctccgcggc
     2461 gaaatcgccg ctgcctgcgg cagctgcgcg atgtggccgc gccgctgcgc cgctgcgcgg
     2521 ctggtcgccg cggtgggcga tgaggattgt ttcaaacatg acgaggaagc tgccttggac
     2581 tggcgtcgaa agaggcgcgc caaggagcgg cgcgcggagg gcttaaccga tcacgaacag
     2641 gggctggccg aactcgaccg gctggccgtt ctccaccagg atttccttga tcacgccgga
     2701 ggcgtcggcg tcgatttcat tgagcagctt catcgcttca atgatgcaaa gggtgtcgcc
     2761 ttccttgacg ctggccccga cttcgacgaa cggcggcttg cccggcgcgg aggcgcgata
     2821 gaaggtgccg accatcggtg acttcacgac gtggcccttc ggggcttccg gggccggggc
     2881 cgcggcggcg gcctgaggag cg
//
LOCUS       DATLGF010000436         4638 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_233312, whole genome shotgun sequence.
ACCESSION   DATLGF010000436 DATLGF010000000
VERSION     DATLGF010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233312"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VM406_13300"
     CDS             complement(<1..628)
                     /locus_tag="VM406_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HVL76984.1"
                     /translation="MRFKKLLTVLAVAAGLTASVAASAKEMRLGLIVPASHQWSQAAT
                     AMGEELKQKSNGKYSVTIFPAGQLGNEAQMLQQLQTGALDMAFMTAAEITNRVPDFGA
                     LFAPYLVRDVREAGVLLNGPTAQKMLERLPREVGAVGVGYGVASMRLMLTAFPANSAK
                     DLKGRKMRITPFPPVRDFYQLLGAASTPMPLTDVYDSLANGQVDGVDAD"
     gene            complement(861..1697)
                     /locus_tag="VM406_13305"
     CDS             complement(861..1697)
                     /locus_tag="VM406_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HVL76985.1"
                     /translation="MNREQEIIARSRAFRKQYVEETPSWYRGEMHLGFTLIFTIGVIW
                     YCATQLEGTTWQEWVFVVIPMFLFGNWAEWAGHRYLLHNPKSWIKPAYKRHVATHHQF
                     FSHKTLDYDGQKEWRALLFPPFAPVLFVLAALPPAILIGMLWSANAGYIAMITMAGYF
                     LMYEGLHTLSHLEHPLLDRIPLINTVRRMHVLHHNPDFMHTRNFNLTFPICDALFGTS
                     DLDKGVFGTLFNGMSDEARKPEDKARVQAQQEARTSANDDPPANAHGEARGHAAKDAR
                     HA"
     gene            1810..2520
                     /locus_tag="VM406_13310"
     CDS             1810..2520
                     /locus_tag="VM406_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVL76986.1"
                     /translation="MSTEKTMRADGLETRARMKAVAQRLFALHGIDGVTAADIVAAAG
                     QRNKASLQYHFGSKEALIGELLVDGAKKVDAFRQALLDEMTAAGGPRTVREVLDAFIR
                     PVHQLTVTETEGSTYLRFLSNVQMTHRQLFRETIGSRWNVGYRRCLDHFRMLLAHVPA
                     PVLEQRMSLVAIYTTTVFSIKEAPLDEAHSRSRLWIPAFTLDNILDTFEAMLSCAPSE
                     ATLARLSGQDQDARGRKT"
     gene            complement(2575..3807)
                     /locus_tag="VM406_13315"
     CDS             complement(2575..3807)
                     /locus_tag="VM406_13315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HVL76987.1"
                     /translation="MRRIAITGIGVVSPLGNSAGEIIENLVRGRPRIRRLEGRDFQRL
                     ANPLGAPSEFDPGPVADAPKLRMMDRSTQFALAATRAALAQAGLDIAAAADRAGVFIG
                     TGSGGAESADSAYRSFYADRSDRVLPFSVLLAMHNAAASWIGLEYGLTGPNLTYSTAC
                     SSSAVAIGEAALRIRSGATDIMIAGGTEAPLCLASMRAWEAMRTIAAADPDDPSASCK
                     PFSKNRSGMVLGEGAAIVILEEWESALARNAPVLAEVSGYGLATDASHLTAPSVQGQA
                     RAMELALADAGMEPEAVDYINAHGTGTVANDVVETAAIRQVFGAHASRLRVSSTKSMH
                     GHLLGAAGALEFAISVLAMNAAIVPPTANLAVPDPECDLDYVPNQARVGSPIRTAMSN
                     SFAFGGTNAVLVASCLCE"
     gene            complement(3804..4088)
                     /locus_tag="VM406_13320"
     CDS             complement(3804..4088)
                     /locus_tag="VM406_13320"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HVL76988.1"
                     /translation="MSTYERLIGILASEYKLKPEQLQPEARLDQIGIDSLGAMELLFK
                     IEDEFGIQVPSDQVQLATVGEVAAYIESLVAQQAPVATPQQAQQPQQARP"
     gene            complement(4085..>4638)
                     /locus_tag="VM406_13325"
     CDS             complement(4085..>4638)
                     /locus_tag="VM406_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVL76989.1"
                     /translation="AAQAAHLWVARQCRPVSALLAGSRQHGDWLATGPIRPGIRMTDD
                     AAFPFRIGNAAGEAHPLAGEGISMALQSAAILADVLIAHAGALDDWRVQQRMREEYAR
                     RWRRHFVRRIRCASLFAHAAMRPGLYRPFLPFLQRHPMVLNVFALWCDKTRAPPALPH
                     LAPAGTGSGASGRLAATQMKEQA"
BASE COUNT          767 a         1500 c         1524 g          847 t
ORIGIN      
        1 tgtccgcgtc cacgccgtcg acctggccgt tggcgagcga gtcatagacg tcggtcagcg
       61 gcatcggggt cgaggcggcg cccagcagct ggtagaagtc gcgcaccggc gggaatggcg
      121 tgatgcgcat cttgcggccc ttgagatcct tcgcgctatt ggccgggaac gcggtgagca
      181 tcaggcgcat cgacgcgacg ccgtagccga cgccgaccgc gccgacttca cgcggcagcc
      241 gctcgagcat cttctgcgcg gtcgggccgt tcagcagcac ccccgcctcg cggacatctc
      301 tcaccagata gggggcgaac agcgcgccga agtccggcac acggttggtg atctcggccg
      361 ccgtcatgaa cgccatgtca agcgcgccgg tctgcagctg ctgcagcatc tgcgcctcgt
      421 tgccgagctg gccggccggg aagatcgtga ccgagtactt gccgttggac ttctgcttca
      481 gttcctcgcc cattgcggtc gcagcctgcg accactgatg gctggccggg acgatcagcc
      541 cgaggcgcat ttcctttgcg cttgccgcga cgctcgcggt caaccctgcg gccacggcga
      601 gaacggtcag tagtttcttg aaacgcattg tcttctcctt gaattggatg agccctggag
      661 gctcgtgctc tcatgatgat gttcttgtca gcgcggcctg ttctgcatgg gccgcgggtc
      721 aactcttgtt cgcttcactc tactcgctac cgggagccgc cgccttgcca acggctccga
      781 ttttttgcca tagagtcttg attgatgtgc gttcgcagca tcgggcgggg gaaggcggcg
      841 gcggcggccg ccgcccgccg tcaggcatgc cttgcatcct tggccgcatg gccgcgcgct
      901 tctccgtgcg cattggcggg aggatcgtcg ttcgcgctcg tccgcgcttc ctgctgggcc
      961 tgcacccgtg ccttgtcctc cggcttgcgc gcctcgtccg acatgccgtt gaacagcgtg
     1021 ccgaacactc ccttgtcgag gtcgctggtg ccgaacagcg catcgcaaat cgggaaggtc
     1081 aggttgaagt tgcgggtatg catgaagtcg gggttgtgat gcagcacgtg catccgccgc
     1141 accgtgttga tcagcgggat gcggtcgagc agcggatgct cgagatgcga aagcgtgtgc
     1201 agcccttcgt acatcaggaa gtaccccgcc atcgtgatca tcgcgatgta gccggcgttg
     1261 gccgaccaca gcatgccgat caggatggcc ggcggcagcg cggcgagcac gaagaggacc
     1321 ggcgcaaacg gcgggaacag cagcgcgcgc cattccttct ggccgtcata gtcgagcgtc
     1381 ttgtgcgaga agaactggtg gtgggtggca acgtggcgct tgtacgccgg cttgatccag
     1441 ctcttcgggt tgtgcaacag gtagcggtgt ccggcccatt cggcccagtt cccgaacagg
     1501 aacatcggga tgacgacgaa cacccattcc tgccaagtgg tgccttccag ctgtgtcgcg
     1561 cagtaccaga tcacgccaat ggtaaaaatg agcgtgaagc cgaggtgcat ctcgccgcgg
     1621 taccagcttg gcgtttcttc cacgtactgc ttccggaatg cgcgcgagcg cgcaatgatt
     1681 tcctgttcgc gattcatgat agtttccttt cgcttgccaa attcagaggg ccggcaggac
     1741 ccgtccctct ggtctccatg cgcacattta agtacactta cttaattaag tctatacgct
     1801 taactatcca tgtcaactga aaaaacgatg cgcgcggacg gcctcgaaac gcgcgcgcgg
     1861 atgaaggcgg tggcgcagcg gctgttcgcg ctgcacggga tcgacggcgt gacggccgcc
     1921 gacatcgtgg cggccgccgg gcagcgcaac aaggcgtcgc tgcaatacca cttcggctcc
     1981 aaggaagcgc tgatcggcga gctgctggtg gacggggcga agaaggtgga tgcgttccgg
     2041 caggcattgc tggacgagat gacggccgcc ggcgggccgc gcacggtgcg cgaggtgctg
     2101 gacgccttca tccgcccggt gcaccagttg accgtgacgg aaaccgaggg cagcacctat
     2161 ctgcgcttcc tgtccaacgt gcagatgaca caccgacagc tgtttcggga aaccataggc
     2221 tcgcggtgga acgtcggcta ccggcgttgc ctggatcact tccgcatgct gcttgcgcat
     2281 gtgccggcgc cggtgctgga gcagcgcatg tcgctggtgg cgatctacac gacgacggtg
     2341 ttctcgatca aggaagcgcc gctggacgag gcgcattcgc gcagccgtct gtggataccg
     2401 gccttcacgc tggacaacat cctcgatacc ttcgaggcga tgctgtcgtg cgcgccgtcg
     2461 gaggcgacgc tggcgcgatt gtccgggcag gatcaggacg ccagggggcg gaagacctag
     2521 ccgggaatgg cgttcgccat gctgtggcgc ccgcccttgc cctgccgcac cgattcactc
     2581 gcaaagacag ctggcgacca gcaccgcatt ggtgccgccg aacgcaaagg aattggacat
     2641 cgcggtccgg atcggtgaac cgacgcgcgc ctggttcggt acgtagtcca ggtcgcattc
     2701 cggatcaggc accgcgaggt tggcggtagg cggcacgatt gccgcgttca tcgcgagcac
     2761 cgagatcgcg aactccagtg cgccggccgc gccgagcaga tgaccgtgca tcgatttggt
     2821 ggagctcacc cgcagccggc tcgcatgcgc tccaaacacc tgccggatcg cggccgtctc
     2881 gaccacgtcg ttggccaccg tgccggtgcc gtgcgcattg atgtaatcga ctgcctccgg
     2941 ctccatgcct gcatcggcca gtgccagctc catcgcgcgg gcctgtcctt gcacgctggg
     3001 cgcggtaagg tgcgacgcat cggtggccag gccgtagccg ctcacctcgg cgaggacagg
     3061 cgcgttccgg gcaagcgcag attcccactc ttccaggatc acgatcgccg cgccttcgcc
     3121 caacaccatg ccgctgcggt tcttcgaaaa cggtttgcag gaagcgcttg ggtcgtccgg
     3181 gtctgcggcc gcgatcgtgc gcatggcttc ccaggcgcgc atcgaagcaa ggcagagggg
     3241 cgcctcggtt ccacccgcta tcatgatgtc cgtggccccg ctgcggatgc ggagcgcggc
     3301 ttcgcctatc gccaccgccg atgacgagca ggccgtgctg taggtcaggt tcggtccggt
     3361 cagcccgtat tcgaggccga tccaggacgc ggccgcatta tgcatggcca gcagcacgct
     3421 gaacggcaac acccgatccg acctgtctgc gtaaaaggat cgataagcgc tgtcggccga
     3481 ttccgctccg ccggaaccgg tgccgatgaa aacgccggcg cggtcggcgg cagcagcgat
     3541 gtccagcccc gcctgcgcca gtgcggcgcg cgtggcggcg agcgcgaact gcgtgctgcg
     3601 gtccatcatg cgcagcttcg gcgcatcggc gaccgggccg gggtcgaact ccgacggcgc
     3661 tccaagcgga ttggccaggc gttgaaaatc cctgccttcc aggcggcgga tccgtggccg
     3721 gccgcgcacc aggttctcga tgatttcgcc tgccgaattg cccaatggcg acacgacgcc
     3781 tatgccggtg atcgcaatgc gcctcatggc cgtgcctgtt gtggctgctg tgcctgctgc
     3841 ggcgtggcca ccggcgcctg ttgcgcgacc aggctttcaa tgtaggccgc cacctcgccg
     3901 accgtcgcaa gctgaacctg atcgctcggc acctggatgc cgaactcgtc ctctatcttg
     3961 aacagcagtt ccatcgcgcc cagcgagtcg ataccgattt gatcgagtcg ggcctcaggc
     4021 tgaagctgtt ccggtttcag cttgtattcg ctggcgagta ttccgatcag gcgttcgtat
     4081 gtgctcatgc ttgctccttc atctgtgtcg ccgcgaggcg gccggatgcg ccgctcccgg
     4141 tccctgcggg agcgagatgc gggagggcgg gcggcgcgcg ggtcttgtcg caccagagcg
     4201 caaagacatt gaggaccatc ggatgcctct gcagaaaggg aaggaaggga cggtacaagc
     4261 ccggacgcat cgccgcatgc gcaaacagcg atgcgcagcg gatgcggcgc acgaagtggc
     4321 ggcgccagcg ccgcgcatac tcttcgcgca tgcgctgctg tacacgccag tcgtcaagcg
     4381 cgccggcatg cgcgatcagc acatcggcca ggatggcggc ggactgcagc gccatgctga
     4441 tgccttcccc ggccaacggg tgcgcctcgc cggccgcgtt cccgatccgg aagggaaagg
     4501 ccgcatcgtc cgtcatgcgt atcccaggcc gtatcggtcc cgtcgccagc cagtctccat
     4561 gctggcggga accggcaagc agcgccgaaa ccggcctgca ctgccgcgcc acccacagat
     4621 gtgctgcctg tgcggcac
//
LOCUS       DATLGF010000437         3755 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_153789, whole genome shotgun sequence.
ACCESSION   DATLGF010000437 DATLGF010000000
VERSION     DATLGF010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153789"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="VM406_13330"
     CDS             complement(<1..1110)
                     /locus_tag="VM406_13330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVL76990.1"
                     /translation="MSADRTSAATSQLLDEDTAELYENAPCAYLSALPDGTLVRVNAT
                     FLAWTGYTRTELLNRKRFQQLLPVVGRIFYDTHFGPLLLMQGFVKEVAFEVICADGTR
                     LPVLLNATLKRDESDRPLVIRVTLLDARSRRTYEEELRLAKRKAEEASESVSRLNASL
                     EERVARRTQERDRIWRNSQDMLIVASLRGELVSFNPAVTRTLGWTSADLEGKRLVALI
                     HPEERRNVGDVVIRLSRGQPVRRFETRNLGKDGEYRWVSWTVMPEGTLLYGIGRDVTE
                     DRKQAEALHKAELALHQAQKMEAIGKLTGGVAHDFNNILQVIAGNLELLKRDVAGSAC
                     ASQRAQIALSAVDRGAKLASQLLAFARRQPLQPVPT"
     gene            complement(1100..1918)
                     /locus_tag="VM406_13335"
     CDS             complement(1100..1918)
                     /locus_tag="VM406_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL76991.1"
                     /translation="MQAKHIIERNNVKVTGSGKQTMLFAHGFGCDQNMWRHVAPAFED
                     DFRVVLFDNVGAGGSDLSAFDPDKYGTLHGYAQDIQEILRALDARDAIFVGHSVSAMI
                     GILAAIEAPELFERLILVGPSPSYINENDYVGGFTREDIEQMLEFLDANHLGWSSRMA
                     PAIMGNADRPALSEELENSFCRTDPAIARHFARTTFLSDHRADLARLAIPSLILQCSD
                     DVIAPDCVGRYMQAQMPGSSFVQMKATGHCPNLSAPEETVQEMRRYLAARNNVG"
     gene            complement(2174..>3755)
                     /locus_tag="VM406_13340"
     CDS             complement(2174..>3755)
                     /locus_tag="VM406_13340"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HVL76992.1"
                     /translation="AHAALAIALVLGLFAARAQVGQLSGTDHALGAKRAVFEMPIVTG
                     LLLGFLASPWFYPSAPSAFSALLGIASAPVLYLFMRGLVEREVFPLFRLIIAFYVVDQ
                     LRRATAGLPGVNRLFLLAEGLLILFFILRFGIRWADRPADLPRTTGTTIALLIGRAML
                     LVAAAVLLAGSAGYAAFADFLLRASLASAFIAITLYALSRAIGGVVHALLYVPPLSFA
                     AGVRRHRDEIAARSRYWLSWGAVAGWLLFTLQAFGVLPLALSWMQAWWDAVWEIGNLQ
                     VSMSSMILFAFILGASYLLSRLVQFLLEEELFSRVQLDRGLPHAVSTTIHYAILLLGF
                     MLALTASGIDMTRFAIVAGALTVGIGFGLQNIVNNFVSGLIVLFERPVKVGDTIQMGD
                     VTGRVLQIGIRATIIESTAGASVIIPNGKLISDQVTNWTRAGHMRQISVPVIAKADRG
                     VDAIKALLLDVARENDKIADLPQPEVLLQKRNIDTLEFELRVWTHDLDAWLTIRSDLT
                     AAADTALRATASGNPPPD"
BASE COUNT          675 a         1173 c         1258 g          649 t
ORIGIN      
        1 cgtcggcaca ggctgcagcg gctggcggcg cgcgaacgcg agcagctgcg aggccagctt
       61 ggcgccgcga tccaccgcgg acaaggcgat ctgcgcgcgc tgcgaagcgc aggcgctgcc
      121 cgccacatcg cgtttcagca gctcgagatt gccggcgatg acctgcagaa tgttgttgaa
      181 atcgtgggcg acgccgccgg tcagctttcc gatggcctcc attttctgcg cctggtgcag
      241 cgccagttca gccttgtgca gcgcttctgc ctgcttgcga tcctcggtga catcccggcc
      301 tatgccgtaa aggagagtgc cttccggcat cactgtccac gatacccagc ggtactcgcc
      361 gtccttgccg agattgcgcg tttcgaagcg gcggaccggc tggccccgcg acagcctgat
      421 gacgacgtcg cccacgttcc tgcgctcttc cgggtggatc agagcgacga gtctcttccc
      481 ttcgagatcg gcgctcgtcc agcccagggt acgggtgacg gccgggttga agctcaccag
      541 ctcgccgcgc agcgaggcca cgatcagcat gtcctgggaa ttgcgccaga tgcggtcgcg
      601 ttcctgcgtg cgccgggcca cgcgttcttc gaggcttgca ttcaggcggc ttaccgactc
      661 gcttgcttcc tcggcctttc gcttggccag ccgcagttct tcctcgtagg tgcgacggct
      721 gcgcgcatcc agcagcgtga cgcggatcac cagcggcctg tcgctttcgt cacgcttgag
      781 cgtggcgttc agcagcaccg gcaagcgcgt gccgtcagcg cagatgactt cgaacgcgac
      841 ttccttgacg aatccctgca tcagcaacag cgggccgaaa tgggtgtcgt agaaaatccg
      901 tcccactacc ggcaacagct gctggaagcg cttgcggttc agcagctcgg tgcgtgtgta
      961 gccggtccag gcgaggaagg tcgcgttgac gcggaccagc gtgccgtcgg gcagcgccga
     1021 caggtatgcg cagggcgcgt tctcgtacag ctcggcggta tcttcgtcca gcagttgcga
     1081 cgtggcggcg gatgttctgt cagccgacat tgttgcgcgc agcgaggtat cgccgcattt
     1141 cctgcaccgt ctcttccggg gcgctgaggt tggggcaatg tccggtggct ttcatctgca
     1201 cgaagctgct gccgggcatc tgcgcctgca tgtagcgacc cacgcaatcc ggggcaatca
     1261 cgtcgtccga gcactgcagg atcagggatg gaatcgcgag gcgggcgaga tcggcccggt
     1321 gatcggacag gaaggtcgtg cgcgcgaagt gccgggcgat ggccggatcc gtgcggcaga
     1381 aactgttttc gagctcttcg gagagtgccg gacggtccgc gttgcccatg atcgccggtg
     1441 ccatcctgct tgaccagccg aggtggttcg cgtcaaggaa ttcgagcatc tgctcgatgt
     1501 cctcacgcgt gaaaccaccc acgtagtcgt tctcgttgat atagctagga gaagggccga
     1561 cgaggatcag ccgctcgaag agctccgggg cttcgattgc cgcaagaatg ccgatcatcg
     1621 cgctcaccga gtggccgacg aagatcgcat cgcgggcatc cagcgcacgc aggatctcct
     1681 ggatgtcctg cgcatagcca tgcagcgtgc cgtatttgtc agggtcgaac gcgctcaggt
     1741 cggagccgcc ggcgccgaca ttgtcgaaca gaacgacgcg gaaatcgtct tcgaaggcag
     1801 gcgcgacatg gcgccacatg ttctggtcgc agccgaatcc gtgggcgaac agcatcgttt
     1861 gtttgccgct gcccgtgacc ttgacgttgt ttctctcgat gatgtgtttg gcttgcatgg
     1921 ctgcgctcag tgtcgctgtc gtgaggaggg aaagtcgccg gtgacaggca ttggtgcgcg
     1981 agaaagcgca gacggggctg cacatgcgca aggccaggtg tcggaatcga atgttgcgtc
     2041 ggggcccccg gcgaggcggc ctatgcaaac cgatatggcg caaaaggggg ctagtctaat
     2101 ggagaatcgc gcggcgtgcc cgcacccgcc tggaggcggc cgggccgccg cagcgtcggt
     2161 caagcgcctg gcctcagtcg gggggcggat ttcccgatgc tgtcgcgcgg agcgcggtat
     2221 ccgccgccgc cgtcaggtcg ctgcgtatgg tcagccatgc gtcgagatcg tgagtccata
     2281 cccgcagctc gaactcgagg gtatcgatgt tgcgtttctg gagcaggact tccggctgcg
     2341 gaaggtcggc gatcttgtcg ttctctctgg cgacatccag cagcagggcc ttgatcgcgt
     2401 ccaccccgcg gtcggctttg gcgatgacgg gcaccgagat ctggcgcata tgccccgcgc
     2461 gcgtccagtt ggtcacctgg tccgagatca gcttgccgtt tggaatgatc acggaagcgc
     2521 cggcggtgct ttctatgatc gtggcgcgga tgccgatctg cagcacgcgg cccgtgacgt
     2581 cgcccatctg tatcgtgtcg cccactttga ccgggcgctc gaacaggacg atcaggccgg
     2641 atacgaagtt gttgacgatg ttctgcagcc cgaagcctat gcccacggtc agtgcgccgg
     2701 caacgatcgc gaaacgggtc atgtcgatgc cgctggccgt cagcgcgagc atgaagccca
     2761 gcagcaggat tgcgtaatgg atggtcgtgg acaccgcgtg cggcaggccg cggtcgagct
     2821 gcacgcgcga gaacagttcc tcttcgagca ggaattgcac caggcgcgaa agcaggtagc
     2881 tggcaccgag tatgaacgcg aagaggatca tgctactcat cgagacctgc agattgccga
     2941 tttcccagac cgcgtcccac cacgcttgca tccagctcag cgcgagcggc agcacgccga
     3001 acgcctgcag ggtgaacagt agccagccgg ccacggcgcc ccagctcagc cagtaacggc
     3061 tgcgcgcagc gatttcatct ctgtgccggc gtacgccggc ggcgaacgac agcggcggca
     3121 cataaagcag cgcatgcacg acgccgccga tcgcgcgcga gagcgcgtac agcgtgatcg
     3181 cgatgaaggc gctcgccagg ctggcacgca gcaggaagtc ggcgaacgcg gcatagccag
     3241 cgctgccggc aaggagcacg gctgccgcaa caagcagcat tgcgcgcccg atcagcagcg
     3301 caatcgtcgt tcccgtcgtg cgcggcagat ccgccggccg gtccgcccag cgtatgccga
     3361 atcgaagaat gaaaaacagg atcagcaggc cctcggccag caggaacagg cggttcacgc
     3421 cgggcaggcc cgccgtcgcc cggcgcaact ggtcgaccac atagaacgcg atgatgagcc
     3481 ggaacagcgg aaagacctcg cgttcgacga ggccgcgcat gaacaggtag aggacaggcg
     3541 ccgaggcgat gccgagcagg gctgaaaaag ccgacggcgc agacggatag aaccaggggc
     3601 tggcaagaaa gccgagcaga aggccggtga cgatcggcat ctcgaagact gcacgcttgg
     3661 cccccagcgc atgatcggtg ccgctcagtt ggccgacctg cgctcttgcg gcgaacaggc
     3721 ccagcaccag cgcgatggcc agcgccgcgt gcgca
//
LOCUS       DATLGF010000438         2767 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_233452, whole genome shotgun sequence.
ACCESSION   DATLGF010000438 DATLGF010000000
VERSION     DATLGF010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233452"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /gene="glgP"
                     /locus_tag="VM406_13345"
     CDS             complement(<1..988)
                     /gene="glgP"
                     /locus_tag="VM406_13345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HVL76993.1"
                     /translation="MINPAVLDEFLYVPRIAYFTMEIALASGIPTYSGGLGVLAGDTM
                     RSAADLELPMIAVTLVSRAGYFRQEIDAHGNQIEHDDWWNPADHAVPLPARIAVSMDT
                     REVWIGAWLHVIESDAGGRIPVLLLDTDLPENSEADRRITDRLYGGDAEYRLRQELVL
                     GVGGVRMLHSLGLRVQRYHLNEGHAALLCLALLRRTRRGERPGSHPQSALYNIDEVRS
                     LCAFTTHTPVEAGHDKFSYETVTRVLGEKRDIALLKELGGDTHLNLTLLALNLSGYVN
                     GVARTHAEQSRRMFPGYRVHAISNGVHPQTWTSPSMATVFHAHFPYWCSEPEM"
     gene            complement(1324..1629)
                     /locus_tag="VM406_13350"
     CDS             complement(1324..1629)
                     /locus_tag="VM406_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012218281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HVL76994.1"
                     /translation="MQTFILLTRLSPEALRAPAAMEDFEKRAVTHIGQECPQVNWLHS
                     YALMGPYDYLDIFTAPDFDAALKVSAMIRTFGRAHSEVWPATDWDNFKQIIRSLPAR"
     gene            complement(1716..2489)
                     /locus_tag="VM406_13355"
     CDS             complement(1716..2489)
                     /locus_tag="VM406_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVL76995.1"
                     /translation="MEHELVNVGIDDGIAVLRLNRPEARNALSRALTGQLDAHLQAVE
                     RRDDVRVIVLCAEGEHFAAGADVREMHNMDQAQVLLADFAGCSRHLACVAMPVIAAVD
                     GCVLGGGCELVEMCDIVIASERSSFGHPEFMLGTMPGAGGTQRLPRVLGKHLAMDLFL
                     TGRRLGAQEAQQHGLVSRVVPAEELMPLALQLARAIAACSAPVARLIKQAVQAGLQDR
                     ESTGLALERALFQQSFALADRAEGMAAFVEKRKPKFRHR"
BASE COUNT          479 a          872 c          924 g          492 t
ORIGIN      
        1 gcatttccgg ctcgctgcac cagtagggaa agtgcgcgtg aaacacggtc gccatgctcg
       61 ggctcgtcca ggtctgcgga tgcacgccgt tgctgatcgc atggacgcgg tatccgggga
      121 acatgcggcg cgattgttcg gcatgcgtgc gtgcaacccc gttcacgtag ccggacaggt
      181 tgagcgcaag cagcgtcaga ttcaggtgcg tgtcgccccc cagttccttc agcagcgcaa
      241 tgtcgcgctt ttctccgagc accctggtga cggtttcata ggaaaacttg tcgtggcccg
      301 cctcgaccgg cgtgtgcgtg gtgaatgcac acaggctgcg cacctcgtcg atgttgtaca
      361 gcgcggattg ggggtgggag ccgggacgct cgccgcgccg cgtgcggcgc agcaaggcca
      421 ggcagagcag ggcggcatgc ccctcgttca ggtgatagcg ctgcacgcga agcccgagcg
      481 aatgcagcat gcgcacgccg cccacgccca agaccagttc ctggcgcagc cggtattctg
      541 catcgccgcc gtacagcctg tcggtgatgc gcctgtcggc ctccgagttt tccggcagat
      601 cggtatcgag cagcagaacc gggatgcggc cgccggcatc gctttcgatc acgtgcagcc
      661 aggcaccgat ccacacttcg cgcgtgtcca tcgataccgc aatgcgggcc ggcagcggta
      721 ccgcgtggtc ggccgggttc caccagtcgt catgttcgat ctggttgccg tgcgcatcga
      781 tctcctgcct gaaatagccc gcccggctga cgagcgtgac ggcgatcatc ggcagttcca
      841 ggtcggcggc cgagcgcatc gtatcgccgg cgagcacgcc aagaccgccg ctataggtgg
      901 gaatgcccga cgccagcgcg atttccatcg tgaagtaggc gatgcgggga acgtagagga
      961 attcgtcgag gactgcagga ttgatcattt tttggcgaaa agcagcttga aatgatggtt
     1021 tgcagcatgt gactgcttgc acgacatgtg catgaggagc tttccaatct acgtcttcga
     1081 aaccgcgaag accgtgagca ggctcaagcc gggcggcaat atgcttgcgg ggggccaagg
     1141 cattgaggta ggacaagcga cggtgaagca ggttgcagcg gcggtctgaa atgaaggtga
     1201 cccgatcggg ggacaagttc gggcgccctt gacgcgcaag cgcgtcgacg gccttgcatt
     1261 gacgcgaagg atttcgcaca gctgcgccgc accgtgtatc gcgagcgctc ctcgcgggca
     1321 gcattacctc gccggcaggg aacggatgat ctgcttgaag ttgtcccagt cggtggcggg
     1381 ccagacctcg gagtgggcgc ggccgaacgt gcggatcatc gccgagactt tcagcgcggc
     1441 atcgaaatcc ggcgcggtga agatgtccag atagtcgtag ggccccatca gcgcatagct
     1501 gtgcagccag ttgacctgcg ggcactcctg gccgatgtgc gtgaccgccc gcttctcgaa
     1561 gtcttccatc gcggccgggg cgcgcaatgc ctccggcgac aggcgcgtga gcaggatgaa
     1621 ggtttgcatg actgcctcgt ctcgttgagc acgctgtcat gctagtcagc ggaccttgcc
     1681 ccggcttgcg cggcatcaaa gcgcgcagga tacgctcagc ggtggcgaaa cttcggtttc
     1741 cgcttctcta cgaatgccgc catgccttcg gcgcggtcgg ccagcgcgaa gctttgctgg
     1801 aacagcgcgc gttcgagcgc cagcccggtg ctctcgcggt cctgcagtcc ggcctgcacg
     1861 gcctgtttga tcaggcgcgc caccggcgcc gagcacgcgg cgattgcgcg cgcgagctgc
     1921 agcgcaagcg gcatcagttc ctctgcggga accacgcgcg aaaccagccc gtgctgctgc
     1981 gcttcctgtg cgccgagccg gcggccggtc agaaagaggt ccatggcgag atgcttgccg
     2041 agcacgcgcg gcaggcgctg ggtgccgccc gcaccgggca tcgtgccgag catgaattcg
     2101 ggatggccga aactggagcg ctcgctggcg atcacgatgt cgcacatttc caccagctcg
     2161 cagcctccgc cgagtacgca accgtccact gcggcgatca ccggcatcgc cacgcacgca
     2221 agatggcgcg aacagccggc aaagtcggcg agcagcacct gcgcctggtc catgttgtgc
     2281 atttcacgga catccgcgcc ggcggcgaag tgctcgcctt cggcgcagag cacgatgacg
     2341 cggacatcgt cgcgccgctc gactgcctgc aggtgcgcat cgagctgacc ggtcagcgca
     2401 cgcgacaaag cgtttcgtgc ctcggggcga ttcagtcgca gtaccgctat gccgtcgtcg
     2461 atgccgacgt tgaccagttc gtgttccatg tcgtccccgc gcaagctgtt gtcaggcgtt
     2521 cattgtggcg acaccgcggc ccggcggcaa acgcgctttg cgtatgcctg gcatgcatgg
     2581 tccgaatccg aattccaccg tcgcatttgc gcgcgatcaa agcccgcccc cgccgagccg
     2641 ccctaaggtg aacatgggca ggcgcgcgct gcctgcatgg agggaagcat ggaatcgacg
     2701 gatgccgccg tgcatctcat gttcggcggc gacgtgatgc tggggcgcgg cgtcgggacc
     2761 gagcttc
//
LOCUS       DATLGF010000439         3165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_11515,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000439 DATLGF010000000
VERSION     DATLGF010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11515"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..1496
                     /gene="der"
                     /locus_tag="VM406_13360"
     CDS             153..1496
                     /gene="der"
                     /locus_tag="VM406_13360"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HVL76996.1"
                     /translation="MKPVIALVGRPNVGKSTLFNRLTRSRDALVADLPGLTRDRHYGE
                     GRVGERPFLVIDTGGFEPVAKEGIVAEMAKQTKQAVAEADVVVFIVDGRQGLTPHDKT
                     ITDFLRKSGRPVMLVVNKAEGMKYTSVTADFYELGLGDPYVISAAHGDGVHDLVNEAL
                     DQASGGRAEQEAEPEQQVKGIRIAIVGRPNVGKSTLINSLLGEERVIAFDMPGTTRDS
                     IEIPFEREGRHYTLIDTAGIRRRGKVFQAVEKFSVVKTLQSISEANVVVLLLDAQQDI
                     SEQDAHIAGFILESGRALVVGVNKWDGLDADRRDQIKTDIERKLNFLFFAKFHFISAL
                     KGSGIAQLMKSVDAAYAAATAKLSTPRLTRALEEAVERQQPRRRGSIRPKMRYAHQGG
                     QNPPIVVIHGNALEAIDDNYKRYLEKHFRETFSLVGTPLRIELRSSKNPFAKGGE"
     gene            1620..1856
                     /gene="hfq"
                     /locus_tag="VM406_13365"
     CDS             1620..1856
                     /gene="hfq"
                     /locus_tag="VM406_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HVL76997.1"
                     /translation="MSNKGQLLQDPFLNALRKEHVPVSIYLVNGIKLQGHIESFDQYV
                     VLLRNTVTQMVYKHAISTVVPARAVNINVDSDSE"
     gene            1884..3008
                     /gene="hflX"
                     /locus_tag="VM406_13370"
     CDS             1884..3008
                     /gene="hflX"
                     /locus_tag="VM406_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HVL76998.1"
                     /translation="MRAALVGVDFGKGDFAASLEELSLLSKSAGAEPVVVITGRRAAP
                     DSALFVGSGKAEEIASAIVAEAIEIVIFNHALSPAQQRNLERHLKIRVVDRTSLILDI
                     FAQRAKSHEGKVQVELAQLQHLSTRLVRGWTHLERQKGGIGLRGPGETQLETDRRLLG
                     DRVKALRTRLDKLHRQRETQRRARERNQAFSVSLVGYTNAGKSTLFNALTKAQAYAAN
                     QLFATLDTTSRRIYLGDAGNVVISDTVGFIRELPHQLVAAFRATLEETIHADLLLHVV
                     DAASPVRMEQIEQVNQVLAEIGAQSIPQILVWNKIDAAGLDPAVERDEYGRIARVFIS
                     AQTGSGLDLLREAVGEFAKASIVAHSDRPPVHVQHDVRPF"
BASE COUNT          637 a          966 c          976 g          586 t
ORIGIN      
        1 ctttctctcc cgcgaggatg gcgcgctcgc cgcgcgtgtt gccaccgatg gcagccgtgt
       61 gctggcgtcg ctgccgatga tgcacgggcg cggcgtgttg attcaaaccc aggccggcag
      121 cgttgtcgcg ctaagggtgg attaaggatt tcatgaagcc ggttattgca ctggtcggac
      181 gcccgaacgt cggaaaatcg acgctgttca accgtctgac gcgctcgcgc gatgcgctgg
      241 tcgcggatct tccggggctg acccgcgacc gacactacgg cgagggtcgc gtcggcgaac
      301 gtccgttcct ggtcatcgac accggcggtt tcgagccggt cgcgaaggaa ggcatcgtcg
      361 cggaaatggc caagcagacc aagcaggcgg tggccgaggc cgacgtggtc gtgttcatcg
      421 ttgatggtcg ccagggcctg acgccgcacg acaagaccat cactgatttc ctgcgcaagt
      481 cgggccgtcc ggtcatgctg gtcgtaaaca aggccgaggg gatgaagtac acctcggtga
      541 ctgcggattt ctacgagctc ggcctgggcg atccttatgt gatctcggcc gctcacggcg
      601 acggcgtgca cgatctcgtc aatgaggcgc tggaccaggc gtcgggcggg cgtgcggaac
      661 aggaagccga gccggagcag caggtcaagg gaatccgcat cgcgatcgtc gggcgtccca
      721 acgtgggtaa atccacgctg atcaattcgc tgctcggcga ggagcgcgtg atcgcgttcg
      781 acatgccggg caccacgcgc gactcgattg aaatcccgtt cgagcgcgag ggcaggcact
      841 acacgctgat cgacacggcc ggcatccggc ggcgcggaaa ggtgttccag gcggtcgaga
      901 aattctcggt cgtcaaaacc ctgcagtcga tctcggaagc aaatgtcgtg gtgctgctgc
      961 tcgatgccca gcaggatatc tccgagcagg atgcccacat tgccggcttc atcctcgagt
     1021 ccgggcgcgc gctggtggtt ggcgtcaaca agtgggacgg cctggacgcg gaccgccgcg
     1081 accagatcaa gacggacatc gagcgcaagc tcaatttcct gttcttcgcc aagttccact
     1141 tcatctcggc gctgaagggc agcggtatcg cgcagctgat gaaatcggtg gacgcggcct
     1201 atgcggcggc caccgcgaag ctgtctacac cccgcctcac acgggcgctg gaggaggcgg
     1261 tcgagcggca gcagccgcgc cggcgcggct ccatccggcc gaagatgcgc tacgcgcacc
     1321 agggcggcca gaatccgccc attgtcgtca ttcacggaaa cgcgcttgag gcaattgacg
     1381 ataattacaa gcgatatctt gaaaaacatt tccgtgaaac tttttcatta gtgggaactc
     1441 cattacgtat cgaactgcgg tccagcaaga acccattcgc caagggcggg gaataagcag
     1501 cggttttctg gcggcgagcg caaaaatcga ttacattggg aatccacttc agcggccgga
     1561 cgcttgataa atgaccggtc gcctccaact ccacatcact acataacaac ggagccgcca
     1621 tgagcaataa agggcaactt ctacaagacc cgttcctcaa cgcactacgc aaagaacacg
     1681 tcccggtttc catctacctc gtcaacggca tcaagctgca gggccacatc gaatccttcg
     1741 atcaatacgt ggttttgctg cgcaacaccg tcacccaaat ggtgtacaag catgccattt
     1801 cgacggtggt gccggcccgt gccgtcaaca tcaatgtcga ttctgattcg gaataaccaa
     1861 gcgttgcccc gcttcacctt cccatgcgcg ctgcactagt cggcgtggat ttcggcaaag
     1921 gggactttgc cgcaagcctg gaagaacttt ccctgctgtc caagtcggcc ggtgccgagc
     1981 ccgtcgtggt catcaccggc cggcgcgcgg cgcctgattc cgccttgttc gtcggctcgg
     2041 gcaaggccga ggaaatcgcc agtgccatcg tcgcagaagc aattgagatc gtcatcttca
     2101 accacgcgct gtcgcccgcg cagcagcgca acctcgaacg ccacctgaag atacgcgtag
     2161 tcgatcgcac cagcctgatc ctcgacatct tcgcccagcg cgccaaaagc cacgaaggca
     2221 aggtgcaggt cgagctcgcc cagctgcagc acttgtcgac ccgtcttgtc cgcggctgga
     2281 cccacttgga gcggcagaag gggggcatcg gcttgcgcgg ccccggcgag acgcagctgg
     2341 aaaccgaccg ccggctgctt ggcgaccggg tcaaggcgct gcgcacgcgg ctggacaagc
     2401 tgcaccgtca gcgtgagacc caacgccggg cgcgcgagcg caatcaggca ttctcggttt
     2461 cgctggtcgg gtatacgaat gccgggaagt cgacgctatt caatgcattg accaaggccc
     2521 aggcgtatgc tgccaaccag ttgttcgcca cactggacac gacgtcgcgc cgcatctatc
     2581 tcggggacgc cggcaacgtc gtcatctccg acacggttgg tttcatccgc gaactgccgc
     2641 accaactggt tgcggcgttt cgcgccacgc tggaggaaac cattcatgcg gatttgctgc
     2701 tgcacgtggt cgacgccgcc agcccggtac ggatggagca gatcgagcag gtcaaccagg
     2761 tcctggccga gatcggtgcg cagagcatcc cgcagatcct cgtctggaac aagatcgacg
     2821 cggcaggcct ggatccggct gtcgaacgtg acgagtatgg tagaatcgcg cgggttttca
     2881 tcagcgccca gacgggaagc ggacttgatc tgctacggga ggccgtcggc gagtttgcca
     2941 aggcaagcat cgtggcgcat tcagaccgcc caccggttca cgttcagcat gatgtgcggc
     3001 ccttttagcc tggcgaatct tcaaatgcgg ttttgatgat tcggcgggaa ggcgcacggt
     3061 ttgcgtcgga cgtcctcagc aatccataga gaagcgagag aatgcccttt cctcttttga
     3121 aaagaatcgg cctgaagttt tccttgaatg atccccagtg gggac
//
LOCUS       DATLGF010000440         2516 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_11517,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000440 DATLGF010000000
VERSION     DATLGF010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11517"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /gene="bamB"
                     /locus_tag="VM406_13375"
     CDS             complement(<1..1018)
                     /gene="bamB"
                     /locus_tag="VM406_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HVL76999.1"
                     /translation="MRIAMKLTCVGMLALLAGCSSLNPFARKTPRNPPAALQEIAPTV
                     AARAAWTASIGSAGRYTFTPAFAGDSVYIAAADGSIARLESGSGRPLWRINAGTALTA
                     GVGTDGSTVAVVADQGVVMAFDADGKQRWTVQASSEVLSAPAVGQGVVVVRSVDNRIA
                     AYEIETGMRRWVAQRTAPPLTLRSAPGIVISDQTAYVALPGGRLSALALNNGAPRWEV
                     AVAEPRGTTELERIADLAGSPVLAGNDVCAVAYQGRLACFDMASGTARWVRETSSEVG
                     VGVDERFVYVADERGAVTAYTRDTGVSAWRNTALANRRLSAPAPVGRTVAVGDFEGHV
                     HFLSR"
     gene            complement(1057..1707)
                     /locus_tag="VM406_13380"
     CDS             complement(1057..1707)
                     /locus_tag="VM406_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVL77000.1"
                     /translation="MAYDLEEQEQLATLKAWWARWGNLITWAAIAVLAAYAGWNGWNY
                     YQRTQAVQAAQLYHELQRAQEAGDPERVQRAAADMTARYARTAYAPMAALAAAHSAYQ
                     NNDAAAAKNHLQWVIDNGAGEQHQALARLRLAGLLLDEKAYDAALKVLDARMPEQFAG
                     LVEDRRGDILAAQNRTADARAAYQAALDKTDASAPGRELIRLKLDAIGGPAEKAAA"
     gene            complement(1720..>2516)
                     /gene="hisS"
                     /locus_tag="VM406_13385"
     CDS             complement(1720..>2516)
                     /gene="hisS"
                     /locus_tag="VM406_13385"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HVL77001.1"
                     /translation="AGADLIAYFEKHASVLDEDARRRLHSNPLRILDTKNPAMQEMVN
                     AAPKLLDYLGAESLAHFEGVQKLLRHYGIPFSINPRLVRGMDYYNRTVFEWVTDQLGA
                     QGTVCGGGRYDPLIEMCGGKPTPAAGFAMGVERLLELMKAAGEQYAPNQCDVYVVHQG
                     EGAQLHAFIAAERLRDAGLDVILHCAAAGGVGSFKSQMKRADGSGAAFAVILGEDEVV
                     RGEATVKALRDADSQQNQTAVPIDGLADYLVDQITGQTDHDHHHHH"
BASE COUNT          377 a          855 c          833 g          451 t
ORIGIN      
        1 cgcgggagag aaagtggaca tggccttcga aatcgccgac agcgacagtc cttccgaccg
       61 gcgccggcgc cgacaggcgg cggttggcaa gcgcggtgtt gcgccatgcg ctcacgccgg
      121 tgtcacgcgt ataggcggtg acggcaccac gctcgtcggc cacgtagacg aagcgttcgt
      181 cgacgcccac gcccacctcg ctcgacgtct cgcgcaccca gcgggccgtg ccgctggcca
      241 tatcgaagca agccaggcgc ccctgatagg cgaccgcgca cacgtcgttg ccggcgagca
      301 ccggggaacc ggccaggtcc gcaatgcgtt cgagttcggt cgtgccacgc ggttctgcaa
      361 ctgccacctc ccagcgcggc gcgccattgt tcagcgcaag ggccgacagg cgcccgcccg
      421 gcaacgcgac gtaagcggtc tggtcggaga tcacgatgcc gggagcgctg cgcaaggtaa
      481 gcggcggagc agtgcgctgg gccacccagc ggcgcatgcc ggtctcgatt tcgtaggcag
      541 cgatccggtt gtcgacgctg cgcaccacga cgacaccctg gccgactgcc ggagccgaca
      601 ggacttcgct ggaagcctgc accgtccagc gctgcttgcc gtcggcgtcg aacgccatga
      661 ccacgccctg gtccgcaacc accgccaccg tgcttccgtc ggttccaaca ccggcggtca
      721 gcgcggtgcc ggcgttgatg cgccacagcg gacgaccgct gcccgattcc agccgggcaa
      781 tgctgccgtc ggcggccgca atgtagacgc tgtcgccggc aaaggccggc gtgaacgtgt
      841 accggccggc gctgccaatg ctggcggtcc acgcagcacg cgccgcgacc gtcggcgcga
      901 tctcctgcag tgcggccggc ggattgcgcg gcgtctttct tgcgaacgga ttgagcgacg
      961 agcagccggc cagcaaggca agcattccga cgcaggtgag cttcatcgcg atacgcattt
     1021 cctctccctt gttctgattc ttgtcgaccg ggccgatcag gctgccgcct tctccgcggg
     1081 gccgccgatc gcatcgagct tcaggcggat cagttcgcgg ccgggggcgc ttgcgtcggt
     1141 cttgtcgagc gctgcctgat aggctgcgcg tgcgtccgcg gtgcggttct gggccgccag
     1201 gatgtcgccg cggcggtcct cgacgaggcc tgcgaactgc tccggcatgc gcgcatcgag
     1261 caccttcagc gcggcgtcgt aggccttttc atccagcagg agtccggcca ggcgcaggcg
     1321 ggcaagcgcc tgatgctgtt cgccggcacc gttgtcgatc acccactgaa ggtggttctt
     1381 ggcagcagcc gcgtcgttgt tctggtatgc gctatgggcg gcggcgagcg cggccatcgg
     1441 tgcgtaggcc gtgcgcgcgt agcgcgccgt catgtcggcc gcggcgcgct gcacgcgctc
     1501 cgggtcgccc gcttcctgag cgcgttgcag ttcgtggtag agctgggccg cctgcaccgc
     1561 ctgggtccgc tggtagtagt tccagccgtt ccagcccgca taggctgcca gcacggcgat
     1621 cgccgcccag gtaatcaggt tgccccacct cgcccaccag gctttcagcg tggccaattg
     1681 ttcctgttct tcgagatcgt atgccatggt gttgcgctct cagtgatgat gatggtggtc
     1741 gtggtcggtc tggccggtga tctggtcgac caggtaatcg gcaagaccgt cgatcggcac
     1801 cgcggtctgg ttttgctggc tgtcggcgtc gcgcagggcc ttcacggtcg cctcgcccct
     1861 gacaacttca tcctcgccga gaatcacggc aaatgcggct ccgctgccgt ctgcacgctt
     1921 catctgcgat ttgaagctgc cgactccgcc ggcggctgca caatgcagga tcacgtccag
     1981 tccggcgtcg cgcaggcgtt cggcggcaat aaaggcatgc agctgcgcgc cctctccctg
     2041 gtgcacgaca tatacatcgc actggttcgg cgcgtactgc tcgccggcag ctttcatcag
     2101 ctccagcaga cgctcgacac ccattgcaaa accggctgcc ggcgtgggct tgccgccgca
     2161 catctcgatc agcgggtcgt aacggccgcc gccgcagacg gtgccctggg cgccgagctg
     2221 gtcggtcacc cattcgaata ccgtgcggtt gtagtagtcc atcccgcgca ccaggcgcgg
     2281 attgatcgaa aacgggatgc catagtggcg cagcagcttc tgtacgccct cgaagtgcgc
     2341 cagcgactcg gcaccgagat aatcgagcag cttcggcgcc gcattgacca tttcctgcat
     2401 cgccggattc ttggtgtcca ggatgcgcag tggattgctg tgcaggcgcc gacgggcgtc
     2461 ttcgtcgagt acgctcgcat gcttttcgaa gtacgcgatc aggtcggcgc ctgcac
//
LOCUS       DATLGF010000441         8324 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_105101, whole genome shotgun sequence.
ACCESSION   DATLGF010000441 DATLGF010000000
VERSION     DATLGF010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 8324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8324
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105101"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VM406_13390"
     CDS             <1..790
                     /locus_tag="VM406_13390"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiD family decarboxylase domain-containing
                     protein"
                     /protein_id="HVL77002.1"
                     /translation="GALQGAPLPVVKCLTSNVRVPAGAEIVIEGRVLPKVREPEGPFG
                     EFPKYYSARENREVIQVDVITHRKSPVFHTIVPAEMEHLLLGSIPREATLLAHLQRSF
                     PGVKDVHLSIGGVGRYHLFVKFEKRREGEAKNVILGAFGAHYDIKQVIVVDTDVDVHD
                     PQQVEWAVATRFQADRDLVVVAGAQGSLLDPSTTVGTETPNGEPPPVYKQGISAKMGL
                     DATKPVAYLEHVFTKVRVPGEDKVDLAQVVRNDVSVDWNTATVK"
     gene            787..1389
                     /locus_tag="VM406_13395"
     CDS             787..1389
                     /locus_tag="VM406_13395"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HVL77003.1"
                     /translation="MNAPADKPRLIIAITGASGTVYGVRMLEQLRASGACETHLVMSA
                     SGALTATQELGMRRGEIEALADVVHNVKDVGATIASGSFRSEGMVIAPCSMKTLASIA
                     HGFADNLVSRAADVILKERRRLVLLARETPLNLAHLRNMTAVTEMGGIVFPPVPAFYS
                     QPETLDDIINHTVGRVLDLFSIPHTRLVKRWEGIGKLSDQ"
     gene            1416..2222
                     /locus_tag="VM406_13400"
     CDS             1416..2222
                     /locus_tag="VM406_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HVL77004.1"
                     /translation="MSQLKHSAQTLLEELDGFRVEIDPARERADIILKRGPFNIISMA
                     QRDQLRLVFESLDENEAVRVIVLRAEGEHFSSGGDIKGFLEASPEHVSKLAWNIAAPT
                     RCSKPVIAANRGFCFGVGFEISLACDFRIATDTTQYALPEQKLGQIPGSGGSARLQKM
                     VGITRTKDIVMRSRRIPGKQAYEWGVATECVPDAELEAATDRLVDELRAFSPIAQRTA
                     KKLLNDTEDAPLSIAIELEGHCYSRLRSSNDFREGVEAFHGKRAPKFNGT"
     gene            2239..3393
                     /locus_tag="VM406_13405"
     CDS             2239..3393
                     /locus_tag="VM406_13405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HVL77005.1"
                     /translation="MKGLLSIADLERAAKKRLPSSIYGYVIGGAEDESSVRGNRAGFE
                     RWGFVPRPLVDVSQRAQETELFGKRYAAPIGISPMGVTGLCRYDGDRALARAARTVGV
                     PFILSGASTTPLEQVAAEMPDVWFQAYIPSRREVIGPLVERVARAGIRTLVVTVDVPI
                     ASVREIEVRNGFSVPLRLSGRLVAGGLARPRWMVQTFAQTLLKQGIPHFENFTATRGG
                     PIITAASGDHRAGRASLCWDDMRWLRTQWDGKLVIKGILHPDDARHARDAGADGVIVS
                     NHGGRQLDGAIAPIEALPGIVEAVPGMTVMVDSGFRRGTDVLKALALGARFVFVGRPA
                     MFGLAVAGERGAVHALRLLQKEIDVNLALLGCASTNGLSRAHLRDRKAER"
     gene            3390..5063
                     /locus_tag="VM406_13410"
     CDS             3390..5063
                     /locus_tag="VM406_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HVL77006.1"
                     /translation="MNQELKERSGGEILVDALRIHGTDTAYCVPGESFLAVIDALYGA
                     KEDIKLIVCRQEGGAAHMAEAHGKLTGKPGICFVTRGPGATNAAIGIHTARQDSTPLI
                     MFVGQVGRDAMGREAWQEIDYRHMYGHIAKWVEQIDDAARIPEYVSRAYHVATLGRPG
                     PVVLALPEDMLTDRVAVPDAPPYRTVEMPPAAAAMEELQSMLYVSKRPLLLLGGGAWS
                     RAAGDDIARFAAAWKLPVACAFRRQDLYDNCDPQYIGEAGLGMDPNLAARIKASDLVI
                     AIGPRLGETTTNGYALFNVPKPAQKLVHVHRDPGELGRVYSADLPIHAGMAEFARAAA
                     ALQPPPDLPWTGWTAAAREDYVNNLRHVPTQSHVDMGAVMQHVRETLPADSIITNGAG
                     NYTLWVQRFYQYRGFRTQLAPTSGTMGYGVPAAIAAKLVHPQRTVVCFAGDGCFLMNG
                     QEMATAVQYGLNIIFIVVNNGMYGSIRMHQEKHYPERVHGTALVNPDFAVLAQSYGIA
                     GERVLRTEDFEAAFARARNAGRPALIELVTDPEIVSPRATVSSLRKAAK"
     gene            5077..6048
                     /locus_tag="VM406_13415"
     CDS             5077..6048
                     /locus_tag="VM406_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HVL77007.1"
                     /translation="MDPENYSTLAAARDWTRGGHQVVLVTVVATWGSSPRPVGAMMCI
                     RDDGLVEGSVSGGCVEDDLIERVRRDGVPQRIATAVYGVSKDEAARFGLPCGGTLRLV
                     VEPLEDIGWVEEVIARIERHELVLRTVDMQSGVQHLAIASGDETCALEGDAFRCVFGP
                     RWRLLLIGAAQTSALAAEIAKTLGFHVIVCDPREEFWRTWPVAGTELMTIMPDDAVLA
                     IEPDPRTAIVALTHDPKLDDMALLEALKSPAFYVGALGSRANTEKRRERLALFDLAPA
                     EIARLHGPVGLRIGSRTPAEIAVSVMAEIISVRSRVAESVRQGVACF"
     gene            complement(6066..7013)
                     /locus_tag="VM406_13420"
     CDS             complement(6066..7013)
                     /locus_tag="VM406_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL77008.1"
                     /translation="MEFRQIQYFICLFEEGTVTRAARRLNIVQPALSMQIAKLEEDIG
                     QQLFQRTKQGMIPTAAARNMYRLFLPIMRDFSNARTQLLSSDGEISGQVNIGMIASVT
                     EGVLADTLSAFSARHPNVGVRVVDGYTATLSDWVAGGQIDAALINKPRRQLALNVEHI
                     ADEEMVLVTGTAYPANLPPRLTLRQITASDVNLVLPTREHGLRGILDSFAQHEDVDLA
                     PIFEVDSLVTSVKLVEQTPQLGTILPHIAVYRGLVEGRLRAHAIVSPRLIRQVVAVSH
                     PRRPLNSATTAFITLLKEQMQARTQGQGTGETVNATPPL"
     gene            7345..>8324
                     /locus_tag="VM406_13425"
     CDS             7345..>8324
                     /locus_tag="VM406_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine methyltransferase"
                     /protein_id="HVL77009.1"
                     /translation="MTIPTEPIGSIPRPLPLIEAMAERGADAPELGPMYEAAIRDTLE
                     RFEATGSPVITDGEQRKYHNFWTYCVQGAPNTAPDGFRIPFAAGHERRMLRLTAGPFR
                     YQRTADAYLEDAMRLTSMPLKQAVISPSALSLMYPAEGVPGYTREQFIEDLLGEHERE
                     VRRCLELGAHSVQIDFTEGRLAMKIDPSGGLLNSFIYLNNLALSRFSPDERRRIGVHT
                     CPGGDCDSTHSADVDYAELLPSLFELKVGSFYIALARESDRPRVLRIVREHMKPDQRI
                     FVGVIDPLDPRVETAEEVCERVLEAAGYIPVERLGTTDDCGFSPFCDDTST"
BASE COUNT         1398 a         2724 c         2801 g         1401 t
ORIGIN      
        1 cggcgcgctg cagggcgcgc cgctgccggt ggtcaagtgc ctgaccagca acgtgcgcgt
       61 gccggccggc gccgagatcg tgatcgaagg tcgcgtgctg ccgaaggtgc gcgagccgga
      121 aggtccgttc ggcgagttcc cgaagtacta cagcgcgcgc gaaaaccgcg aagtgatcca
      181 ggttgacgtg atcacccacc gcaagtcgcc ggtcttccac accatcgtgc cggccgagat
      241 ggagcacctg ctgctcggct cgatcccgcg cgaggcgacc ctgctggccc acctgcagcg
      301 cagcttcccg ggcgtgaagg atgtgcacct gtcgatcggc ggcgtggggc gctaccacct
      361 gttcgtcaag ttcgaaaagc gccgcgaggg cgaggcgaag aacgtgatcc tgggcgcatt
      421 cggcgcgcac tacgacatca agcaggtgat cgtggtcgac accgacgtgg acgtgcacga
      481 tccgcagcag gtcgaatggg cggtggccac gcgcttccag gccgaccgcg acctcgtcgt
      541 cgtggccggg gcccagggtt cgttgctcga cccgtccacc acggtgggaa cggagacgcc
      601 caatggcgag ccgccgccgg tctacaagca gggcatcagc gcgaagatgg gcctggacgc
      661 gaccaagccg gtggcctacc tcgagcatgt gttcaccaag gtgcgcgtgc cgggcgagga
      721 caaggtggat ctggcgcaag tggtcaggaa cgacgtttcc gtcgactgga acacggccac
      781 cgtcaaatga acgccccggc agacaagccg cgcctgatca tcgccatcac cggcgcgagc
      841 ggcaccgtgt acggcgtgcg catgctggag cagctgcgcg cgtcgggcgc ctgcgagacg
      901 cacctggtga tgtccgcatc cggtgcgctg acagcgaccc aggagctggg catgcggcgc
      961 ggcgagatcg aggcactggc ggacgtggtg cacaacgtca aggatgtcgg cgccaccatc
     1021 gcttccggat cgttccgttc ggaaggcatg gtgatcgcgc cgtgctcgat gaagacgctg
     1081 gccagcatcg cccacggatt tgcggataat ctggtcagcc gcgccgccga cgtgatcctg
     1141 aaggagcggc gccggctggt gctgctggcg cgcgaaaccc cgctgaacct cgctcatctg
     1201 cgcaacatga ccgccgtcac ggaaatgggg ggcatcgtgt ttcccccggt gcctgccttc
     1261 tactcgcagc ccgagacgct cgacgacatc atcaaccaca cggtcgggcg ggtgctcgac
     1321 ctgttctcga ttccgcatac ccggctggtc aagcgctggg aaggaatcgg caagctgtcc
     1381 gaccaataaa cgacatcatc cgaaggagat acgccgtgtc gcaactgaag cattccgccc
     1441 agaccctgct cgaggaactc gacggctttc gcgtcgaaat cgatcccgcg cgcgaacgcg
     1501 ccgacatcat cctgaagcgc ggcccgttta acatcatctc gatggcccag cgtgaccagc
     1561 tgcgcctggt gttcgagtcg ctcgacgaaa acgaagccgt acgcgtgatc gtgctgcgcg
     1621 ccgaagggga gcacttctcc agcggcggcg acatcaaggg tttcctcgaa gcgagccctg
     1681 agcatgtgtc caagcttgcc tggaacatcg cggcccccac gcgctgcagc aagccggtga
     1741 tcgccgccaa ccgcggcttc tgcttcggcg tcggtttcga aatctcgctg gcctgcgact
     1801 tccgcatcgc caccgacacc acccagtacg cgctgcccga gcagaaactc ggccagatcc
     1861 ccggatccgg cggctcggcg cgcctgcaga agatggtggg catcacccgc acgaaggaca
     1921 tcgtgatgcg ctcgcgccgc attccgggca agcaggccta tgagtggggc gtggcgaccg
     1981 agtgcgtgcc ggatgcggaa ctggaagccg ccaccgaccg cctggtcgat gaactgcgcg
     2041 cgttctcgcc gatcgcccag cgcaccgcga agaagctgtt gaacgacacc gaggacgcac
     2101 cgctgtcgat cgcgatcgaa ctggaaggcc attgctacag ccgcctgcgt tcgtcgaacg
     2161 atttccgcga aggggtggag gccttccacg gcaagcgcgc gccgaagttc aacggcacct
     2221 gagcggaagg tatccggcat gaagggcttg ctctcgattg ccgacctgga gcgcgccgcg
     2281 aagaagcggt tgccgtcgag catctacggc tacgtgatcg gcggtgccga agacgagagc
     2341 tcggtgcgcg gcaaccgtgc cggcttcgag cgctggggct ttgtgccgcg gccgctggtg
     2401 gacgtgtcgc agcgcgcgca ggaaaccgaa ttgttcggca agcgctacgc ggcgccgatc
     2461 ggcatcagcc cgatgggcgt gaccgggctg tgccgctatg acggcgaccg cgcgctggcg
     2521 cgtgccgcgc gtacggtcgg cgtgcccttc atcctgagcg gcgcctcgac cacaccgctg
     2581 gaacaggtcg ccgccgagat gccggatgta tggttccagg cctacatccc gtccaggcgc
     2641 gaggtgatcg gcccgctggt cgaacgggtt gcgcgtgccg gaatcaggac gctggtcgtc
     2701 acggtcgacg tgccgatcgc ctcggtgcgc gagatcgagg tgcgcaacgg cttttcggtg
     2761 ccgcttcggc tgtccggccg gctggttgcc ggcggcctcg cacgaccgcg ctggatggtg
     2821 cagacctttg cgcaaacctt gctgaagcag ggcatccctc atttcgaaaa cttcaccgcc
     2881 acgcgcggcg gtcctatcat caccgccgcc tcgggagatc atcgcgccgg ccgtgccagc
     2941 ctgtgctggg acgacatgcg ctggctgcgc acgcaatggg atggcaagct ggtgatcaag
     3001 ggcatcctgc accccgacga cgcacggcac gcgcgtgacg ccggcgccga cggcgtgatc
     3061 gtttccaatc acggcgggcg ccagctcgac ggcgcgattg cgccgatcga ggccttgccc
     3121 ggcatcgttg aagccgtgcc cggcatgact gtgatggtgg acagcggctt tcggcgcggc
     3181 accgacgtgc tcaaggcgct ggcgctgggc gcgcgcttcg tgttcgtcgg ccgacccgcg
     3241 atgttcggcc tcgcggtcgc aggcgagcgg ggggccgtgc atgcgctgcg cctgttgcag
     3301 aaggaaatcg atgtgaacct cgcattgctg gggtgcgcgt caacgaatgg attgtcgcgc
     3361 gcccatctgc gcgatcgaaa ggcagaacga tgaaccaaga attgaaggaa cgcagcggcg
     3421 gcgagatcct ggtcgacgcg ttgcgcatcc atggcaccga cacggcctat tgcgtgccgg
     3481 gcgagagctt tctcgccgtg atcgacgcgc tctacggcgc gaaggaagac atcaagctga
     3541 tcgtctgccg ccaggaaggc ggcgcggcgc acatggccga ggcgcacggc aagctgaccg
     3601 gaaagcccgg catctgcttc gtcacgcgcg ggcccggcgc gaccaatgcc gcaatcggca
     3661 tccataccgc gcgccaggat tcgacgccgc tgatcatgtt cgtcggccag gtcggccgcg
     3721 acgcgatggg acgcgaggcc tggcaggaga tcgattaccg gcacatgtat ggccatatcg
     3781 ccaagtgggt ggagcagatc gacgatgccg cgcgcattcc cgagtatgtg agccgcgcct
     3841 accatgtcgc caccttgggc cggcccggcc cggtggtgct ggcgttgccc gaagacatgc
     3901 tgaccgatcg cgtcgctgtt cccgatgcgc cgccttaccg cacggtcgag atgccgccgg
     3961 cggctgcagc gatggaggaa ctgcagagca tgttgtacgt gtcgaagcgg ccgttgctgc
     4021 tgctgggcgg cggcgcatgg tcgcgcgctg caggcgacga catcgcccgc tttgccgcgg
     4081 cgtggaagct gccggtggcc tgcgcattcc gccgccagga cctgtacgac aactgcgacc
     4141 cgcaatacat cggcgaagcc ggcctcggca tggatccgaa cctggccgcg cgcatcaagg
     4201 catcggacct ggtgatcgcc atcggtccgc gcctcggaga aaccacgacc aacggctacg
     4261 cgctgttcaa tgtgccgaag cctgcgcaga agctggtgca tgtgcatcgc gatccgggcg
     4321 agctcgggcg cgtctacagc gccgacctgc ctatccatgc gggcatggcc gagttcgccc
     4381 gcgccgccgc cgcactgcag ccgcctcccg acttgccgtg gaccggctgg acggccgccg
     4441 cgcgcgagga ctacgtcaac aacctgcgcc atgtgccgac gcaatcgcat gtggacatgg
     4501 gcgcggtgat gcagcatgtg cgcgagacgc tgcctgccga ctccatcatc accaacggcg
     4561 ccggcaacta cacgctgtgg gtgcagcgct tctaccagta ccgcggtttc cgcacccagc
     4621 tcgcgccgac cagcgggacc atgggctacg gcgtgccggc ggcgatcgcg gccaagctcg
     4681 tgcatccgca gcgtacggtg gtctgcttcg ccggcgacgg ctgcttcctg atgaacggcc
     4741 aggagatggc gaccgccgtg cagtacggcc tgaacatcat cttcatcgtg gtcaacaacg
     4801 gcatgtacgg cagcatccgc atgcaccagg aaaagcatta cccggagcgc gtgcacggca
     4861 ccgccctggt caatccggac tttgcggtgc tggcgcagtc ctacggcatc gccggcgaac
     4921 gcgtgctgcg caccgaggat ttcgaagccg cgttcgcacg cgcgcgcaat gccggacgtc
     4981 cggcgctgat cgaactggtc accgatcccg agatcgtgtc gccgcgcgcg accgtgtctt
     5041 cgctgcgcaa ggcggcgaag tagaaggagt gttgcattgg acccggaaaa ctattccacg
     5101 ctggccgcgg cccgcgactg gacgcgcggc ggtcatcagg tcgtgctggt gaccgtcgtc
     5161 gccacctggg gttcctcgcc gcgcccggtc ggcgcgatga tgtgcattcg cgacgacggg
     5221 ctggtcgagg gttcggtctc gggtggatgc gtggaggacg acctgatcga gcgcgtgcgc
     5281 cgcgacggcg tgccgcaacg catagccacg gcggtctatg gcgtgagcaa ggatgaggcg
     5341 gcgcgcttcg gactgccctg cggcggcacg ctgcgcctcg tggtggaacc gcttgaggac
     5401 atcggctggg tcgaagaggt catcgcgcgc atcgagcggc acgaactggt gctgcgcacg
     5461 gtggacatgc agagcggtgt ccagcatctc gcgatcgcct ccggggacga gacctgcgcg
     5521 ctcgaaggcg atgccttccg ctgcgtgttc ggcccgcgct ggcggctgct tttgatcggc
     5581 gccgcgcaga cttcggcatt ggccgccgag atcgccaaga cgctcggctt ccatgtgatc
     5641 gtctgtgatc cgcgcgagga gttctggcgc acctggccgg tggccggcac ggagctgatg
     5701 accatcatgc ccgacgatgc ggtgctggca atcgagccgg acccgcgcac ggccatcgtc
     5761 gcgctgaccc atgaccctaa gctggatgac atggccttgc tggaagcgct gaagtcgccg
     5821 gccttctatg tcggcgcgct cggctcgcgc gcgaatacgg aaaaacgccg cgagcggctc
     5881 gcgctgttcg atctggcgcc ggccgaaatc gcccgcctgc acggaccggt cggcctgcgc
     5941 atcggcagcc gcacgccggc cgagattgcg gtgtcggtca tggcggagat catcagtgtg
     6001 cgcagccgcg ttgccgagtc ggtgcggcag ggcgtcgcct gcttctgaac gctggctcca
     6061 ggcaattaaa gcggcggggt agcgttcacc gtctcgccgg taccttgccc ctgcgtgcgt
     6121 gcctgcatct gctccttgag cagggtgatg aatgccgtgg tcgccgagtt cagcgggcgg
     6181 cgcgggtgcg ataccgccac tacctggcgg atcaggcgcg gcgagacgat cgcatgggcg
     6241 cgcaggcggc cttcgaccag gccgcgatag accgcgatgt gcggaaggat ggtccccagc
     6301 tgcggcgtct gttcgaccag tttcaccgag gtcaccagcg aatccacttc gaatatcggc
     6361 gcgaggtcga catcctcgtg ctgggcaaag ctgtccagga tgccgcgcag cccatgctcg
     6421 cgcgtgggca gcaccaggtt cacatcggaa gcggtgatct ggcgcagcgt caggcgcggc
     6481 ggcaggttgg ccggataggc cgttccggtc accagcacca tttcctcgtc ggcgatgtgt
     6541 tcgacgttca gcgccagctg gcggcgcggc ttgttgatca gggccgcgtc gatctgtccg
     6601 cctgccaccc agtccgagag cgtcgcggtg tagccgtcca cgacgcgcac gccgacgttc
     6661 ggatggcgcg ccgagaacgc gctcaatgtg tcggcgagca cgccctcggt caccgaagcg
     6721 atcatcccga tattcacctg gccgctgatc tccccgtcgc tgctgagcag ctgggtgcgc
     6781 gcattggaga agtcgcgcat gatcggcagg aacagccggt acatgttgcg cgccgcggcg
     6841 gtggggatca ttccctgctt ggtgcgctgg aacagctgct ggccgatgtc ttcctccagc
     6901 ttggcgatct gcatgctcag cgccggctgc acgatgttca ggcgccgcgc cgcgcgggtc
     6961 acggtgcctt cctcgaacag gcagatgaag tactggatct ggcggaattc catggttgcg
     7021 ctcgcgtcgt ccttgttcgg atagtcgccc cgatgatatc acctgaaatg atgaaaaccg
     7081 gttttcgttt gttgcctatc acggtttgca gcgcagcccc gccgccgaag tgcaggtgcg
     7141 ccaatgcgcg gccccatcaa ctgtattgat ccacgcgatg gcggaaatta tctgaggtga
     7201 tgaacggatt gcatttgcgc ggacgaccca cctttggatt tcccgtatgc aaatccgatc
     7261 ttgcgcaacg cccggtcgcc tcgttcgcgg tggaaaacca tgaggccgat ataatcggcg
     7321 ggtcctcttt cgcatccgac gcatatgacg attcccaccg agccgatcgg cagcatcccg
     7381 cgtcctcttc cgctgatcga ggcgatggcc gagcgcggcg ccgatgcccc cgagctgggg
     7441 ccgatgtacg aagcggcgat acgtgacacc cttgaacgct tcgaggccac cggctcgcct
     7501 gtgatcaccg acggcgagca gcgcaagtat cacaacttct ggacctattg cgtgcagggg
     7561 gcgccgaaca cggccccgga cggcttccgc attccgtttg ccgccggcca tgaacgccgc
     7621 atgctgcggc tgacggccgg tccattccgc tatcagcgca ctgcggacgc ttatctggaa
     7681 gacgcgatgc gcctcacatc gatgccgctc aagcaggcgg tcatctcccc ttcggcgctg
     7741 agcctgatgt acccggccga gggcgtgccc ggctacacgc gcgaacagtt catcgaggac
     7801 ttgctgggcg agcatgagcg cgaagtgcgg cgctgcctgg aactcggtgc gcatagcgtg
     7861 cagatcgatt tcaccgaggg gcgcctggcg atgaagatcg atccttcggg aggcttgctc
     7921 aacagcttca tctacctgaa caacctcgcg ctgtcccgct tctcgcctga cgagcggcgg
     7981 cgcatcggcg tacatacctg tccgggcggc gactgcgatt cgacacacag cgccgacgtc
     8041 gattacgcgg agctgctgcc cagcctgttc gagctgaaag tcggcagttt ctacatcgcg
     8101 ctggcgcgcg agtccgatcg gccgcgtgtg ctgcgcatcg tccgcgagca catgaagccg
     8161 gaccagcgca tctttgtcgg cgtcatcgat ccgctcgacc cccgcgtcga aaccgcggag
     8221 gaagtctgcg agcgcgtgct cgaagccgcc ggctatattc cggtggagcg gctgggcacg
     8281 acagacgact gcggattctc gccgttctgc gacgacacat ctac
//
LOCUS       DATLGF010000442         7706 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_154114, whole genome shotgun sequence.
ACCESSION   DATLGF010000442 DATLGF010000000
VERSION     DATLGF010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 7706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7706
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154114"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2086)
                     /locus_tag="VM406_13430"
     CDS             complement(<1..2086)
                     /locus_tag="VM406_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HVL77010.1"
                     /translation="MLLQALRMTRRDWRAGELRFLLLALIVAVSSLSSVGFFVDRMRA
                     GLNRDAHQLLGADLVVNADQPVSALWRNEAERRGLNVSDTVTFPSMAIAGEGDDFQSQ
                     LVSLKAVGPGYPLRGALRLVAPDGERAAAGIPAAGTAWVDPGLLGALQIEPGAVLRLG
                     DMRFRVAEQISIEPDRGAGFLSFAPRVMIALPDLAATNLVQDGSRVTYRLLVAGEPAA
                     VQDFQRWIETRIDEDRVRGVRLESLESGRPEMRATLDRAEQFLSLVGLLSAMLAAVAI
                     AMAARRFMLRHLDACAMLRCLGLTQNQVTALYLIEFLVVGIAGSVLGAAVGYAAHFVL
                     INGVAALVQTQLPAPSLLPAVQGVATGLVLLIGFALAPILQLRNVPHNRVIRREQDVP
                     QPLTLATYVLGAVAFVGLLLWQAGNVRLGLLTAGGFLAGFAVFALVGWLAVKSLGFVR
                     NRLDHPSWRFAVTALQRRTGASVVQVVALSLGLMALLLLTVIRSDLVHAWRESTPPNA
                     PNRFVINIQPEQQREIAARLAGLGVESDLLYPMIRGRLVQLNDRPIGPESYEEDRAQR
                     LVNREFNLSTMLAIPSQNQVVAGRWFDDSRPEASVEEGLARTLGISLGDRMRFDIGGQ
                     QVEAEVTSLRRLEWGSMRVNFFVILNPALMQDMPHTWITAFHLPAGQEGFVNALMSDF
                     PNLTVVDIGSIVR"
     gene            complement(2086..2670)
                     /locus_tag="VM406_13435"
     CDS             complement(2086..2670)
                     /locus_tag="VM406_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HVL77011.1"
                     /translation="MGDLTSALALAGGLAWASGVRLYMTLFIAGWFGRAGWIELPPAL
                     QVLESPWVMAVTGALLLVEFLADKVPGVDSAWDAVQTFIRVPAGAILGAAALGHMAPE
                     WVAAAGLLGGTFAAGAHATKAGSRALINTSPEPFSNWAASVSEDVAVVTALWVAFFHP
                     WLLLAFLVFFFILALWLLPKLWRGLRWLFRKLSV"
     gene            complement(2663..3022)
                     /locus_tag="VM406_13440"
     CDS             complement(2663..3022)
                     /locus_tag="VM406_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77012.1"
                     /translation="MRHSALPVVLIVLGAVWLLNSLDWLPDAHWLWILGLAGAGVGIL
                     LLDGITKSSVVAGPLLILAAVMSFARQYHDLGWRFIFPVMLIAAGLLMLVARSGAIPP
                     SRQLERGAGNEGGGRHG"
     gene            complement(3032..4051)
                     /locus_tag="VM406_13445"
     CDS             complement(3032..4051)
                     /locus_tag="VM406_13445"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HVL77013.1"
                     /translation="MDDALRSLLMRMPKAELHMHIEGSLEPELIFALAARNRVTLAYP
                     SIEALRSAYAFTDLQSFLDIYYAGASVLLQEQDFYDMTWAYLQRAHADNVRHAEIFFD
                     PQTHTERGVAFATVIDGIHRALRDAEQQLGVSSALIMCFLRHLSEEEAFATLEQALPH
                     RDKFIGVGLDSSERGHPPEKFARVYARCRELGLHLVAHAGEEGPPAYIRSALDVLKVE
                     RVDHGVRCLEDPELTVRLARERVPLTVCPLSNVKLRVFDRAADHNLARLLDAGLAVTI
                     NSDDPAYFGGYVNDNYIRMFEVLPLGREHARTLARNSFAAAFLGPERKEAFLAEVDRF
                     FAEAT"
     gene            complement(4128..4520)
                     /locus_tag="VM406_13450"
     CDS             complement(4128..4520)
                     /locus_tag="VM406_13450"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HVL77014.1"
                     /translation="MSALSEEQIREIKRMLDEREQELRADLERESGQKREVTDHAPEV
                     PDPGDASFVSLALDLGNAEMTRDFVELRAIGAARARIEDGSYGECSVCGVEIPYARLQ
                     VQPTAERCAPCQEIFERTHADVGRGATL"
     gene            complement(4549..5568)
                     /locus_tag="VM406_13455"
     CDS             complement(4549..5568)
                     /locus_tag="VM406_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006754830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL77015.1"
                     /translation="MDVRLKKLSEQTIVLTGATSGIGLVTAREAARRGAKLVLVARNE
                     DALKQLQKELQAKGCEAICVAADVANDQEMRRVSQAAIDRFGGFDTWVNNAGVSIFGR
                     LEDVPLEDQRRLFETNFWGVVHGSMVAVEHLKQRGGALINIGSEVSLRAVPLQGMYSA
                     SKHAVKGFTDSLRSELEEVKAPVSVTLVKPAAIDTMFLEHAKNVMDVEAQLPPPVYAP
                     EAVAEAILHAAEHPRRDIYVGGASKLVATASAAMPRMVDRIMSLIYRQQRSNRPVNPA
                     HRDSLHAPGPDMKERGGQERHVFETSAYTKASLRPRLATTLALGAGLAGVAAATLLRK
                     RHRTA"
     gene            5797..6495
                     /locus_tag="VM406_13460"
     CDS             5797..6495
                     /locus_tag="VM406_13460"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HVL77016.1"
                     /translation="MCGRFVMNHPPELLQRWYEAARMPEFTSRFNIAPGQTIHVLRDM
                     EGSRIFSTMRWGFIPSWADDAGNAPMLHNARAESVAEKPMFRHAFKSRRCLIPASGYY
                     EWRTDPGRKTRRPYYLSLPDGAPMSFAGLWELTTTASGEQVETCAVVTTKANALIEPI
                     HHRMPVVLDRESWPLWLSRDAQTAALLVLLRAYPAERMQMWEVDSAVNLTDNDHCGLY
                     APRAGRAGVQAAML"
     gene            6507..7139
                     /locus_tag="VM406_13465"
     CDS             6507..7139
                     /locus_tag="VM406_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77017.1"
                     /translation="MPEITLMLDEGQHLRLNGEYKRMSMAWLRSRPDAPPSFEQWLSD
                     RLASPSPSSAPAVRQAEALREELRAVDAIEKLVTSLQAHGFALAQIDRRERDDAASAL
                     AGALAAALKLSPARSRRLAELAAYYARSPREVADAGGVGVTQRAQTALIDACRALAER
                     TLRIRDRLGPERALGRIEGSLAIVMMLHVISRQTGREQAEAFRRRLEEHP"
     gene            7296..7574
                     /locus_tag="VM406_13470"
     CDS             7296..7574
                     /locus_tag="VM406_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77018.1"
                     /translation="MQYARNQFGVPQYPQDDVRRLFVLLCAVDLLERPTASAIADLTG
                     HDRESIDADIMRLREELHVGIARVGEVYHIESWGDVLKKNGVKRILKH"
BASE COUNT         1409 a         2627 c         2481 g         1189 t
ORIGIN      
        1 ggcgcacgat gctgccgatg tcgaccacgg tcaggttcgg gaaatcgctc atgagcgcat
       61 tgacgaagcc ctcctgcccc gccggcaggt ggaaggcggt gatccaggta tgcggcatgt
      121 cctgcatcag cgccggattc aagatcacga agaaattcac ccgcatcgag ccccactcga
      181 gccggcgcag cgaggtcacc tcggcctcca cctgctggcc gccgatgtcg aaacgcatgc
      241 ggtcgccgag actgatgccg agcgtcctgg cgagcccttc ctcaaccgac gcttccgggc
      301 ggctgtcgtc gaaccagcgt cccgcgacca cctggttctg cgacggaatc gcaagcatcg
      361 tggacaggtt gaattcgcgg ttgaccagcc gctgtgcgcg gtcttcctcg taggattcgg
      421 gtccgatggg tcgatcgttc aactggacca gccggccccg gatcatcggg tacagcaggt
      481 cgctctccac gcccaggccc gccaggcgtg cggcgatttc gcgctgctgc tccggctgga
      541 tgttgatcac gaagcggttt ggagcgttcg gcggcgtcga ttcgcgccac gcgtgcacga
      601 ggtcgctgcg gatgacggta agcaacagca acgccatcag gccgagcgac agggccacga
      661 cctgcaccac gctcgcaccg gtgcgacgct gcagcgcagt aaccgcgaag cgccaggacg
      721 gatgatccag ccggttgcgc acgaatccga gcgacttcac ggccagccat ccgaccagcg
      781 cgaacaccgc gaagccggcc aggaagccgc ccgcggtcag caggcccagc cgcacgttcc
      841 ccgcctgcca cagcagcagg ccaacgaagg cgaccgcccc caggacgtag gttgccagcg
      901 tgagcggctg gggaacatcc tgttcacgcc ggatgacccg gttgtgcggg acgttgcgaa
      961 gctgcaggat cggcgcgagc gcgaatccga tcagcaggac cagtccggtt gccacgccct
     1021 gcacggccgg cagcagcgac ggcgccggca gctgggtctg caccagcgcc gccacgccgt
     1081 tgatcagcac gaaatgcgcg gcatagccga ccgccgcccc cagcacgctg ccggcaatgc
     1141 cgacgacgag gaattcgatc aggtacagcg ccgtcacctg gttctgcgtc aggccgaggc
     1201 aacgcagcat cgcgcaggcg tcgaggtggc gcagcatgaa gcggcgcgcg gccattgcga
     1261 tcgccaccgc ggccagcatc gccgacagca gcccgaccag cgacaggaac tgttcggcgc
     1321 ggtccagcgt ggcgcgcatt tccggtcggc ccgattccag cgattcgagg cgcacgccgc
     1381 gcaccctgtc ttcgtcgatg cgcgtctcta tccaccgctg aaagtcctgc acggcggcag
     1441 gctcgccggc caccagcagc cggtaggtga cgcgcgaacc gtcctggacc agattggtcg
     1501 cggccagatc gggcagcgca atcatcacgc gcggtgcaaa actgaggaag ccggcgccac
     1561 ggtccggctc gatcgagatc tgctcggcga cgcgaaagcg catgtcgccc agccgcagca
     1621 ctgcgccggg ctcgatctgc agtgcgccga gcagcccggg gtcgacccag gcggtacccg
     1681 cggccggaat gccggcagca gcacgctcgc cgtccggggc gacgaggcgt aatgcacccc
     1741 gcaatggata acccggtccg accgccttga gcgacacgag ctggctctgg aagtcgtcac
     1801 cttcgccggc aatcgccatg ctcgggaagg tcacggtgtc ggaaacgttc agtccgcgcc
     1861 gctctgcctc gttgcgccac agggccgaga ccggctggtc ggcgttgacc accagatccg
     1921 cgcccagcag ttgatgggcg tcgcgattca ggccggcgcg catgcggtcg acgaaaaagc
     1981 cgaccgacga cagggacgac acggcaacga tcagggccag cagcaaaaaa cgcagctcgc
     2041 cggcgcgcca gtcacggcgc gtcatacgca atgcttgcaa cagcatcaga cactcaactt
     2101 tctgaagagc cagcgcagcc cgcgccaaag ctttggcagg agccacaagg caaggatgaa
     2161 gaaaaagacg agaaaggcca gcaacagcca gggatggaag aacgcgaccc acagtgccgt
     2221 aaccacggcc acgtcctcgg ataccgaagc cgcccagttg ctgaacggtt ccggagaggt
     2281 attgatcagc gcgcggctgc ccgccttggt cgcgtgcgcg ccggccgcga aagtgccgcc
     2341 cagcaaaccg gcggccgcaa cccattcggg cgccatgtgg ccgagcgccg ccgcgccgag
     2401 aatcgcgccg gccggcacgc ggatgaaggt ctgcaccgcg tcccaggccg agtccacgcc
     2461 aggcaccttg tcagccagga actcgaccag cagcagcgcg ccggtcacgg ccatcaccca
     2521 tggcgactcc agtacctgca gcgcgggcgg cagttctatc cagcctgcgc ggccgaacca
     2581 gccggcaatg aacagcgtca tgtacaggcg cacgccgctg gcccaggcca gcccgccggc
     2641 cagcgcgagc gcggatgtca ggtcacccat ggcgccctcc gccttcgttc ccggcgccgc
     2701 gctcgagctg gcgcgacggc ggaatcgcgc cggaacgcgc caccagcatc agcaggccgg
     2761 ctgcgatcag catgaccggg aagatgaagc gccagccgag atcgtggtat tgccgcgcga
     2821 agctcatcac ggcagcaaga atcagcagcg gaccggcgac cacggaggat ttcgtgatgc
     2881 cgtcgagcaa aagaatgccg acgcccgcgc ctgccagccc gagaatccac agccagtgcg
     2941 cgtccggcag ccagtccagg ctgttgagca gccagacagc gccaagcacg atcaggacaa
     3001 cgggcagcgc ggagtgtctc atggcgcgtt cctacgtcgc ctccgcaaag aaacggtcga
     3061 cctcggccag gaaagcctcc ttgcgctcgg gccccaggaa tgcggcggcg aagctgttgc
     3121 gcgccagcgt gcgcgcatgc tcgcgcccca gcggaaggac ttcgaacatc cggatgtagt
     3181 tgtcgtttac gtagccgccg aaataggccg ggtcgtcgga gttgatggtg acggcaaggc
     3241 cggcgtcgag caggcgggca agattgtggt ccgccgcacg gtcgaacacg cgcagcttca
     3301 cgttggacag cggacacacg gtcagcggca cgcgctcgcg cgcaagtcgc acggtcagct
     3361 ccggatcctc caggcagcgc acgccatggt cgacacgttc cactttcagc acatcgagcg
     3421 ccgagcggat gtaggccggc ggcccttcct ccccggcatg cgcgaccagg tgcaggccca
     3481 gttcgcggca gcgcgcgtaa acgcgcgcaa atttctccgg cggatggccc cgctcggagg
     3541 aatccaggcc cacgccgatg aacttgtcac gatgcgggag cgcctgctcc agggtcgcga
     3601 acgcctcctc ctccgagagg tggcgcagga aacacatgat cagcgcgctg gaaacgccaa
     3661 gctgctgttc cgcgtcgcgc agagcgcggt gaatgccgtc gatcacggtg gcgaaggcca
     3721 ccccgcgctc ggtatgggtc tgcggatcga agaaaatctc cgcatggcgc acgttgtcgg
     3781 catgggcgcg ctgcaggtag gcccaggtca tgtcgtagaa atcctgctcc tgcagcagga
     3841 ccgaggcgcc cgcgtagtag atgtcgagga aggactgcag gtcggtgaac gcgtaggcgc
     3901 tgcgcagcgc ctcgatcgac ggataggcga gcgtgacccg gttgcgcgcg gccagcgcga
     3961 aaatcagctc cggctccagc gagccttcga tgtgcatgtg caactccgcc ttgggcatcc
     4021 tcatcagcag gctacgcagc gcgtcgtcca tcgtcgatcc tttcgcggca tgtgtcgatg
     4081 ctgcgattgt acgtgcaaga cccgcctttc ggtcgatccg gaacagacta cagcgtggct
     4141 ccccgtccga cgtcggcatg cgtcctctcg aagatttcct ggcatggcgc gcagcgttcg
     4201 gcggtaggct ggacctgcag ccgcgcgtac gggatctcga cgccgcacac cgagcactcc
     4261 ccgtagctgc cgtcctcgat gcgcgcgcgc gccgcgccga tggcgcgcag ctcgacgaag
     4321 tcacgcgtca tctcggcatt gcccaggtcg agagcgaggc ttacgaaaga agcatcgccc
     4381 gggtccggga cttccggtgc gtggtcggtc acttcacgct tctggccgga ttcccgctcc
     4441 agatccgcgc gcaactcctg ctcgcgctcg tcgagcatcc gcttgatctc gcgaatctgc
     4501 tcctcactca gtgctgacat gtcggcgtac tcccatggtt gatgttgatc aggcggtgcg
     4561 atggcgcttg cgcagcagcg tggcggcggc gacgccggcc agtccggcgc ccagcgccag
     4621 cgtggtcgcc agccgagggc gcaggctcgc ctttgtgtag gcgctggttt cgaagacgtg
     4681 gcgctcctgg ccgccgcgct ccttcatgtc ggggccaggg gcatggaggc tgtcacggtg
     4741 cgccggattg accggccggt tcgagcgttg ctgacggtag atcagcgaca tgatccggtc
     4801 gaccatgcgc ggcatggcag cggatgccgt cgcaaccagt ttcgaggcgc cgccgacgta
     4861 gatgtcgcgg cgcgggtgct cggccgcatg caggatcgcc tcagcgaccg cttccggcgc
     4921 atacaccggc ggcggcagtt gcgcctccac gtccatgacg tttttcgcat gctcgaggaa
     4981 catcgtgtcg atggcagccg gcttgaccag tgtgaccgag accggcgcct tcacctcctc
     5041 cagttccgaa cgcaacgaat cggtgaatcc cttcaccgcg tgcttggatg ctgaatacat
     5101 cccctggagc ggcaccgcgc gcagcgaaac ttcgctgccg atgttgatca gcgcgccgcc
     5161 gcgctgcttc aggtgttcga cggccaccat cgatccatgc accacgcccc agaaattcgt
     5221 ttcgaacagc cggcgctggt cctccagcgg gacatcctcc aggcggccga aaatcgacac
     5281 gcctgcattg ttgacccagg tgtcaaagcc tccgaagcgg tcgatggcgg cctgcgacac
     5341 cctgcgcatt tcctgatcgt tggccacatc ggccgcgacg cagatggcct cgcagccctt
     5401 ggcctgcaat tccttctgca actgcttcaa cgcatcttca ttgcgcgcca ccagcaccag
     5461 cttcgcgccg cgccgcgccg cttcacgcgc ggtcaccagt ccaatcccgc tggttgctcc
     5521 ggtcagcacg atggtttgtt cggaaagttt cttcaagcgc acatccatgt tcgttctcct
     5581 tcacgatgtt gtcagcgaaa accgtgcccg gaggtagcac acgcgggaat ggccgcggga
     5641 accggccgcg cactcgacgg cgcgcgacgt ttttaccggc atgctttcct ttttcctgtc
     5701 gctactgccg ccgcttgtgg cggtggaggg tttgagcaag cttcaagcct ggaattcaag
     5761 gttcccgctc tgctgtatcc gtcctacaat tgaaacatgt gcggacgctt cgtcatgaat
     5821 caccctcccg agcttctcca gcgctggtac gaggctgcgc gcatgcccga gttcacctcc
     5881 cgcttcaaca tcgcgcccgg tcagacgatt catgtcctca gggacatgga aggcagccgc
     5941 atcttttcca cgatgcgctg gggcttcatt ccctcctggg ccgacgatgc gggcaacgca
     6001 ccgatgctgc acaatgcccg ggcggaatcg gtggccgaga aaccgatgtt ccgccatgcg
     6061 ttcaaaagtc gccgctgcct gatcccggcc tccggctact acgagtggag aaccgatccc
     6121 ggccgcaaga caaggcgacc gtactacctg agcctgcccg acggcgcgcc gatgtcgttc
     6181 gccgggctgt gggagctcac gacaactgcc agcggcgagc aggtcgagac atgcgcggtc
     6241 gtcacgacca aggcgaatgc gctgatcgaa ccgatccacc accggatgcc ggtcgtgctg
     6301 gaccgggaaa gctggccgct gtggctctcc cgcgatgccc agaccgcggc cctgcttgtc
     6361 ctgctgcgcg cctacccggc cgagcggatg cagatgtggg aggtcgattc cgccgtgaat
     6421 ctcaccgaca acgaccattg cggtctgtac gccccgcgcg ccggccgcgc gggtgtccag
     6481 gccgccatgc tgtaacaacc atcaagatgc ccgaaatcac cctgatgctg gacgagggcc
     6541 agcacctgcg cctgaatggc gaatacaagc gcatgtcgat ggcctggctg cgcagccggc
     6601 ccgacgcacc gccaagcttc gaacaatggc tgtccgaccg gctggcgtcc ccctccccaa
     6661 gctccgctcc ggccgttcga caggccgaag cactgcgtga agaactgcgc gccgtcgacg
     6721 ccatcgaaaa gcttgtgacg tcgctgcagg cgcacggctt tgcgctggcc cagattgacc
     6781 ggcgcgaacg cgacgatgcg gcatccgccc tcgcgggtgc gctcgccgcc gcgctgaagc
     6841 tgtcgcccgc acgcagccgg cgactggcgg aactggcggc ctactatgcg cgcagcccgc
     6901 gtgaagtcgc cgatgccggc ggggtcggcg tgacgcaacg cgcacagacg gccctcatcg
     6961 atgcctgtcg ggcgctggcc gaacgcacgc tgcgcatccg ggaccgactg ggtccagagc
     7021 gcgcgcttgg ccggatcgaa ggcagcctcg cgatagtgat gatgctgcac gtcatctcgc
     7081 ggcagacggg gcgagagcag gccgaggcgt tccgcaggcg gctcgaggaa catccataga
     7141 ggcactggcg acatgcggct gacacggtgc gccgacgcag acggcacaaa gggtacaagc
     7201 ggtacaaacc gcagaaacgg ccggcgagtg ccgcgcagga atgcatgacg ctggcgtctc
     7261 tcagttcgca acccgttcca accacgaggt atgccatgca atatgcaagg aaccagtttg
     7321 gcgtccccca gtatccgcag gacgatgtgc gtcgcctgtt cgtccttctc tgcgccgtcg
     7381 atttgctcga gcgcccgacc gcctccgcca tcgccgacct caccgggcac gaccgcgaat
     7441 cgatcgatgc cgacatcatg cggctgcgcg aggaactgca tgtgggaatc gcccgcgtcg
     7501 gcgaggtcta tcacatcgag tcgtggggcg acgtgctgaa gaagaatggc gtcaagcgca
     7561 tcctcaagca ctgagcagcg ccgaacttgc aaaggctgca gcgctccaaa caggcaaagg
     7621 agcgagccat gaaacaggat ggaaaaagcc ggcaggtgca cggcgcggag ccggaggccc
     7681 cccatgccgg ccggccgttg gacggc
//
LOCUS       DATLGF010000443         5790 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_142186, whole genome shotgun sequence.
ACCESSION   DATLGF010000443 DATLGF010000000
VERSION     DATLGF010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142186"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VM406_13475"
     CDS             <1..188
                     /locus_tag="VM406_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HVL77019.1"
                     /translation="VTDFNKYGGDITFRITRPADNTLIAMAKSGFVFFNFRTKKVVPM
                     PESFHTKFEKVNWLGPA"
     gene            263..709
                     /locus_tag="VM406_13480"
     CDS             263..709
                     /locus_tag="VM406_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HVL77020.1"
                     /translation="MDSTRSDCFTRGGLLAALALAAAPLLPATAADGKLEPKTGKASY
                     YGPQFTGRPMADGTPFDPESNHAASRTLPLGTWVKVTNLENGRSEVVQIRDRGPYIDG
                     RIIDLSPRTARNLDMIEQGVVQVEVVPLNEEERQFALTEAELTSGE"
     gene            904..2154
                     /locus_tag="VM406_13485"
     CDS             904..2154
                     /locus_tag="VM406_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HVL77021.1"
                     /translation="MHLPPDAIATTVESVYRAESRRVLATLIRLLRDFDLAEEALHEA
                     FRAALEQWPRDGMPENPRAWLVSAGRFKAIDGIRKQARFDPLDESHEQIAAVDPLMPW
                     DKESVEDDRLRLVFTCCHPALAPDAQVALTLREVCGLTTEEIARAFLVPAATLAQRIV
                     RAKAKIRDARIPYEVPAASELSERLDAVLRVIYLVFNEGYSASFGDSLTRHDLSAEAI
                     RLGRLLCDLLPDPEAQGLLALMLLQESRRAARANADGELILLEHQDRSLWNREQIAEG
                     CALVERALSTRRFGPYTLQAAIAAVHAEAPDAGSTDWAQIVGLYDVLVRAAPSPVIEL
                     NRAVAVAMRDGPAAGLVLIDAILGRGALQDYHLAHAARAELCRRLGRDAEACASFSRA
                     LDLVKQEPERRFLARRLEALQQGR"
     gene            2282..2626
                     /locus_tag="VM406_13490"
     CDS             2282..2626
                     /locus_tag="VM406_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HVL77022.1"
                     /translation="MKYLCLIYNDERALATIPQHELDAIDAECRAHCAALERSGQLLA
                     AERLQPVATATSIRMRNGKASVTDGPFAETREQLGGFYLLQARDLNEAMRIAEKIPPA
                     RLGTIEVRPVLE"
     gene            2713..3063
                     /locus_tag="VM406_13495"
     CDS             2713..3063
                     /locus_tag="VM406_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HVL77023.1"
                     /translation="MKYLCLVYSEESKLHTLPDSPKDAECMAYAQRVDASGRMIAAEA
                     LQPVHTATTVRVRSGKLSITDGPFAETREQLAGFYLVEADNLDQALEIAAGIPAARVG
                     SVEVRPVRTLQPDE"
     gene            3085..3531
                     /locus_tag="VM406_13500"
     CDS             3085..3531
                     /locus_tag="VM406_13500"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HVL77024.1"
                     /translation="MLTENRNAQAEADIRALMNEWTDAFMAKDVNRIIASYTQDVVAF
                     DAILKLQFLDRESYRKHWEACMAMCPGPVEFELRDLRVTAAEQIAFAHYICRCGGTDD
                     KGETHSSWMRASLGLRREQDRWLIAHEHFSSPFDPATGKAMFDVTP"
     gene            3547..3960
                     /locus_tag="VM406_13505"
     CDS             3547..3960
                     /locus_tag="VM406_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVL77025.1"
                     /translation="MKIQPYLFFNGRCEEAIRFYQEVLGAEITALMRYKESPDPAMTP
                     PGAEDKIMHASVRIGDTELMASDGDCSGKPSFAGFSVALTVAGAAEAERVFNALAHGG
                     QVQMPPAETFFSPRFGMLADRYGLNWMIMAEMPQQ"
     gene            3973..4395
                     /locus_tag="VM406_13510"
     CDS             3973..4395
                     /locus_tag="VM406_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HVL77026.1"
                     /translation="MRFMMLVIPKGYESAAPGTMPDPKQVEAMMKYNTALQEAGVLLA
                     LDGLHPPSMGARVSFSGGKPTVTDGPFTETKEVLGGYWMIRVNSREEAIEWAKRCPMS
                     DNEIIEIRQVQEFDDFTPEVKEAAKGFDAMHTQLEQAR"
     gene            4547..5392
                     /locus_tag="VM406_13515"
     CDS             4547..5392
                     /locus_tag="VM406_13515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HVL77027.1"
                     /translation="MHPHKHSTIDVLIPTCNRAYALAVTLASLLGQTWHSLRVVISDQ
                     SDRDDVFDTPEVAAVLRVLRATGRQVETHRHLPRRGMAEQRAFLLAQATAARCLFLDD
                     DVILENDAIARMAEMLEFEQCGFVGSALHGLSFLDDIRPEQQIIEFWEDPVEPESVTP
                     GGPEWERHHLHSAANLFHLQARLGLTKADTRLYKVAWVGGCVLFDTEKLREAGGFDFW
                     SELPHEHCGEDVLAQLRVMARHGGCAIIPSGAYHMELPTTVTTREIDAPMILKVGAAF
                     STEAS"
     gene            5389..>5790
                     /locus_tag="VM406_13520"
     CDS             5389..>5790
                     /locus_tag="VM406_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HVL77028.1"
                     /translation="MSSAPEDMRRIVVVRPNAVGDFIYALPCLHALRATWPQARITYV
                     GKQWHADFLAGRPGPIDEVAVIPPCPGVGAPVDAEFDQTRLERFIETMRAARFDLALQ
                     IYGGGRYSNPFTRRLGARLCIGMKADDAEPLD"
BASE COUNT         1057 a         1900 c         1870 g          963 t
ORIGIN      
        1 gcgtgacgga cttcaacaag tacggcggcg acatcacctt ccgcatcacg cgccctgccg
       61 acaacacgct gatcgcgatg gccaagtccg gcttcgtttt ctttaacttc aggacgaaga
      121 aggtggtgcc gatgccggaa agcttccaca cgaagttcga gaaggtgaac tggctcggac
      181 ctgcttgagg cagcgcagag cgccgaactc ccgaatttgc atttcatcca acggtcgttc
      241 ccgaccgcaa tggaggaagg caatggacag tacccgatcc gactgtttca cgcgtggcgg
      301 actgctcgcc gcgctggcac tggctgccgc ccccctgctt cccgctaccg cggccgacgg
      361 caagctggag ccgaagacgg gcaaggcttc ctactatggt ccgcaattca ccggccgccc
      421 gatggccgac ggcaccccgt tcgatcccga gtccaatcat gcggccagcc gcaccttgcc
      481 gctgggcacc tgggtcaagg ttaccaacct ggaaaacgga cgcagcgaag tggtgcagat
      541 ccgcgaccgc ggcccttaca tagacgggcg catcatcgac ctttccccgc gcaccgcgcg
      601 caatctggac atgatcgaac agggcgtcgt gcaggtcgag gtggtcccgc tcaacgaaga
      661 ggaacgccag ttcgcgctga ccgaggcgga actcaccagc ggcgaatagc gccgtccccg
      721 tcttttgcac tgcccttgcc gccgcgcgga atccgcgctg taatccgctt tgcgttgtcg
      781 atcctgccac cgacggcacg acaatatggc accacagcaa gccaggagca aagacaccat
      841 gcctgatccc tcccattgcg gcgcacggcg cgggcgcgct cctggccgtt agccggggca
      901 gccatgcacc tgccaccgga cgccatcgcg acaacagtgg aatcggtcta tcgggccgag
      961 tcccgccgcg tgctcgccac gctgatccgc ctgctcaggg atttcgacct ggccgaggaa
     1021 gcgctgcacg aggcgtttcg cgcggcactc gagcaatggc cgcgcgacgg catgccggaa
     1081 aatccgcgcg cctggctggt atcggccggc cgcttcaagg cgatcgacgg catccgcaag
     1141 caggcccgct tcgatccgct tgacgaaagc cacgaacaga ttgcggctgt cgacccgttg
     1201 atgccatggg acaaggagag cgtggaggac gaccggctgc gcctggtgtt cacctgctgc
     1261 cacccggccc tggcgcccga cgcccaggtc gcgctgacgc tgcgcgaggt gtgcgggctg
     1321 accaccgaag aaatcgcgcg cgccttcctg gtgccggctg ccacgctggc ccagcgcatc
     1381 gtgcgggcga aggcgaagat ccgcgatgcg cgcattccgt acgaggtgcc ggcggcaagc
     1441 gagctgtccg agcggctgga cgcggtgctg cgcgtgatct atctggtgtt caacgaaggc
     1501 tactccgcat cgttcggcga ctcgctgacg cgccacgacc tgtccgccga agccatccgg
     1561 ctcggccgcc tgctgtgcga tctgctgccc gacccggaag cgcaagggct gctggcgctg
     1621 atgctgctgc aggaatcgcg ccgcgcggcc cgcgcgaatg cggacggcga actgattctg
     1681 ctcgagcacc aggaccgcag cctgtggaac cgcgagcaga ttgccgaggg ctgcgcgctg
     1741 gtggagcgcg cgttgagcac gcgccgtttt ggaccctata cgctgcaggc tgcgattgcc
     1801 gccgtgcatg cggaagcgcc cgacgcaggc tccaccgact gggcgcagat cgtcggtctg
     1861 tatgacgtgc tggtgcgcgc cgcaccttcg ccggtgatcg agctgaaccg ggcggtggcg
     1921 gtggcgatgc gcgacggccc ggcggcgggg ctggtcctga tcgacgcgat cctggggcgc
     1981 ggcgcattgc aggattacca cctcgcgcat gcggcacgtg ccgaattgtg ccggcggctc
     2041 ggccgcgatg cggaagcatg tgcgtcgttt tcccgcgcgc tcgacctggt gaagcaggaa
     2101 cccgagcggc gctttctcgc gcggcggctg gaagcgttgc agcagggccg ctagcgcacc
     2161 tgcctccgcg gcgtgccgac gtcatccctt tctttctggt gatgccttgg cattcgcgtg
     2221 tcgaatccgg ccggcctcgt acgacaagtg cataaccgcc cctgaaaacg aggagaagcc
     2281 gatgaaatac ctgtgcctga tctacaacga cgagcgcgcg ctggcgacta tcccccagca
     2341 cgaactggac gcgatcgatg ccgaatgccg ggcgcactgc gcagcgctgg aacgcagcgg
     2401 gcagctgctt gccgctgaac ggctgcagcc ggttgccact gcgacgtcga tccgcatgcg
     2461 caacggcaag gcgtccgtca ccgacggccc ctttgccgaa acccgcgagc agctcggcgg
     2521 tttttacctg ctgcaggcgc gcgacctgaa cgaagccatg cgcattgccg agaagattcc
     2581 gccggcgcgg ctcggcacca tcgaagtgcg tccggtgctt gaatgatttt tccctgatcc
     2641 atgtcgattc cggcacgccg cgatcgatta aacagtaccc accgattaca cagtacgcac
     2701 ccggagatgc cgatgaaata cctgtgcctg gtctacagcg aggaaagcaa gctgcatacg
     2761 ttgcccgaca gcccgaaaga cgcggagtgc atggcctacg cgcaacgcgt cgacgccagc
     2821 ggccgcatga tcgccgccga ggcgctgcag ccggtgcata cggccaccac ggtgcgcgtg
     2881 cgcagcggca agctgagcat caccgacggc ccgtttgccg agacgcggga gcagctggcc
     2941 gggttttatc tcgtcgaggc cgacaacctc gaccaggcgc tggagattgc ggccgggatt
     3001 ccggccgccc gagtggggag tgtcgaagtg cgtccggtgc gcacgctgca gccggacgaa
     3061 tgaacgaacc cagaggagaa catcatgctg accgaaaaca gaaacgccca ggcggaggcc
     3121 gatatccgcg cgttgatgaa tgaatggacc gacgccttca tggccaagga tgtaaaccgc
     3181 atcattgcca gctatacgca ggacgtggtc gccttcgacg ccattctcaa gctgcaattc
     3241 ctcgaccgcg agtcgtatcg caagcactgg gaagcctgca tggcgatgtg tcccggcccg
     3301 gttgagttcg agctgcgcga cctgcgggtg acggcggccg agcagatcgc gtttgcgcac
     3361 tacatctgcc gttgcggcgg caccgacgac aagggcgaga cgcactcgag ctggatgcgc
     3421 gcctcgctcg gcctgcgcag ggaacaggac cgctggctga tcgcccacga gcatttctcc
     3481 tcgccgttcg accccgcgac tggcaaggcg atgttcgacg tcacgccctg aaaagaagga
     3541 gaacccatga agatccagcc ctatctcttt ttcaacggcc gctgcgagga ggcgatccgg
     3601 ttctaccagg aagtgctcgg cgccgagatc acggcgctga tgcgctacaa ggaaagcccg
     3661 gacccggcga tgacgccgcc cggcgcggag gacaagatca tgcacgcgag cgtgcgcatc
     3721 ggggataccg agctgatggc ctccgacggc gactgcagcg gcaagccgag cttcgccggt
     3781 ttttcggttg cgctgacggt tgccggtgcg gccgaagccg agcgcgtatt caacgcgctc
     3841 gcccacgggg ggcaggtcca gatgccaccc gccgagacct tcttctcgcc gcgcttcggc
     3901 atgctcgccg accgctacgg gctgaactgg atgatcatgg ccgagatgcc gcagcaatga
     3961 ggagaacaca ccatgcgttt catgatgctt gtcattccaa aagggtacga aagcgccgcc
     4021 ccgggcacga tgcctgatcc gaagcaggtc gaggcgatga tgaagtacaa cacggcactt
     4081 caggaagccg gcgtgctgct ggccctggat ggtctgcatc cgccgtcgat gggtgcgcgc
     4141 gtgagttttt ccggcggcaa gcccacggtc accgacggcc cattcacgga aaccaaggaa
     4201 gtgctcggcg gctactggat gatccgggtc aattccaggg aggaggcgat cgaatgggcc
     4261 aagcgctgcc cgatgtcgga caacgagatc atcgagatcc gccaggtgca ggagttcgat
     4321 gatttcacgc cggaagtgaa ggaagcggca aagggcttcg acgcgatgca cacacagctg
     4381 gagcaggcac gctagcgagt ccgtcttgcc tccggtgtaa aaagcgttca cggtgccttc
     4441 atcgtgaacg cggctttcgc acgacgctca tttccggtgg gcccggcgtt tcgccgtgca
     4501 cacttcctgc ttgcagactc tcttccgtac aacaagaggt gtctgcatgc atccccacaa
     4561 gcattcaacg atcgacgtac tgattcccac ctgcaatcgc gcatatgcgc ttgcggttac
     4621 gctcgcctcg ctgctgggcc agacatggca cagcctgcgt gtcgtgattt ccgaccagtc
     4681 ggatcgcgac gacgtgttcg acacgccgga agtggccgcg gtgctgcgcg tgctgcgcgc
     4741 aaccgggcgc caggtcgaaa cccatcgcca tctgccgcgc cgcggcatgg ccgagcagcg
     4801 ggcctttctg ctggcgcagg ccactgcggc gcgctgcctg ttcctcgatg acgacgtgat
     4861 tcttgagaac gatgcaattg cgcgcatggc cgagatgctc gaattcgaac aatgcggctt
     4921 cgtcggcagc gcgctgcacg gactgtcctt cctcgacgac atccggcccg aacagcagat
     4981 catcgaattc tgggaggacc cggtggagcc ggagtccgtc acgcccggcg gacccgaatg
     5041 ggagcgtcac cacctgcaca gcgccgccaa tctgttccat ctgcaagcca ggctcggcct
     5101 cacgaaagcc gacacccggc tctacaaggt cgcatgggtg ggcggctgcg tgctgttcga
     5161 cactgaaaag ctgcgcgagg ccggcggctt cgatttctgg agcgaactgc cgcacgagca
     5221 ttgcggcgag gacgtgctcg cgcagctgcg cgtaatggcg cgccacggag gctgcgcgat
     5281 cattccttcc ggcgcctacc acatggaatt gccgaccacg gtgaccacgc gcgagatcga
     5341 cgcacccatg atcctcaaag tgggcgcggc cttcagcacg gaagcctcat gagttcggcg
     5401 ccggaagata tgcgccgcat cgtcgtggtg cggccgaacg cggtgggcga cttcatctat
     5461 gcactgccct gcctgcacgc attgcgggcc acctggccgc aggcacggat cacctatgtt
     5521 ggcaagcagt ggcatgcgga tttcctcgcg ggccgcccgg ggccgatcga cgaagttgcc
     5581 gtgattccgc cctgccccgg tgtgggtgcg ccggtcgacg ccgagttcga ccagacccgg
     5641 ctcgagcgct tcatcgagac catgcgcgcg gcgcgcttcg acctcgcgct gcagatctac
     5701 ggcggcgggc gttattcgaa tccgttcacc cggcgactgg gcgcgcgcct gtgcatcggc
     5761 atgaaggccg acgacgccga gccgctcgat
//
LOCUS       DATLGF010000444        14107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_154243, whole genome shotgun sequence.
ACCESSION   DATLGF010000444 DATLGF010000000
VERSION     DATLGF010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154243"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VM406_13525"
     CDS             complement(<1..673)
                     /locus_tag="VM406_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HVL77029.1"
                     /translation="MLPNKIRQDWFSNVRGDLLSGLVVALALIPEAIAFSIIAGVDPK
                     VGLYASFCIAVVIAFAGGRPGMISAATGAMALVMVTLVKEHGLQYLLAATLLTGVLQI
                     AAGVLKLGSLMRFVSRSVVIGFVNALAILIFMAQLPELTNVSWTVYAMTAAGLAIIYL
                     FPRITKAVPSPLVCIMVLTAVSMAFGLDIRTVGDMGELPDSLPVFLLPDVPLNWETLA
                     IILPYS"
     gene            1020..2639
                     /locus_tag="VM406_13530"
     CDS             1020..2639
                     /locus_tag="VM406_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL77030.1"
                     /translation="MTSPPRSEPAIRKPPASESLSALAPLQHPVFRMLWTVWLVANIS
                     MWMNDVAAAWIMTSLTSVPIWVALVQTAATLPVFLLGLPSGAMADIFDRRRYFIVTQF
                     WVAIVALILSVVILAGVLTPGLLLALTFANGIGLAMRWPVFAAIVPELVPRPQLPSAL
                     ALNGVAMNLSRIVGPLLAGALIATAGSAWVFVLNAVLSIACAFVIMRWRREHVPHPLG
                     RERLTRAIRVGLQFVGQSTRLKAVLLRIFIFFFHSTALLALLPLVARDLGAGDAATFT
                     LLLATMGAGAIAAAMLLPRVRRTWTRDTVVIGGTTLQSVSILVVALAPNTAIAVFGMF
                     AAGVAWISTANSLSVSAQLSLPDWVRARGMSMYQMAIMGGSAAGAAAWGQVATMTDVD
                     TALIASAVTGVAAMLAAYRIIVDRSIEEDLTPSREFKAPVAEAPPPSGNIVVTIEYLI
                     DPQRSAEFRELMQESRRSRLRQGALSWSLLRDVTVPGRFVEQIVDESWTEHLRRFDRV
                     TAGDVALRERKLAFHVGDTPPVITRCVVEAS"
     gene            complement(2662..3441)
                     /gene="surE"
                     /locus_tag="VM406_13535"
     CDS             complement(2662..3441)
                     /gene="surE"
                     /locus_tag="VM406_13535"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HVL77031.1"
                     /translation="MQKRAIERVLLTNDDGIDAPGLRALEEVAASIADEVWVVAPEHD
                     RSGTSHSISLHAPLRVHDLGNRRYSVSGTPGDCVVMALQHIMADARPSLVLSGINRGA
                     NLGVETVFSGTVGAAMTGLLLGLPSIALSQAFTDRQAVRWDTARALAPDVIRRLWDAG
                     WNANACLNVNFPDVPAEEAGPVTFTRQGAGLVKNIDVLARTDPRGFTYYWLQFNRQPY
                     EDAPESETAAVARGRVSVTPLQFERTNDTAFAGLEQAMPQG"
     gene            complement(3541..4398)
                     /locus_tag="VM406_13540"
     CDS             complement(3541..4398)
                     /locus_tag="VM406_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVL77032.1"
                     /translation="MRVHHLNCISSCPLGGKLMDGQTSTVFGRGGLCCHCLLVETEAG
                     LVLIDTGFGLRDVADPRGRLSHFFLALLSPDFREEMTAVRQIEALGFSATDVRHIVLT
                     HLDFDHAGGLDDFPEATVHMLQQERDDALAQRSWLDRQRYRPQQWSSRKRWKVYRLGR
                     GEGWYGFECVRDLAGVPPEILMVPLPGHTYGHAGIAVRQADRWLLQAGDAYFFHREMN
                     LSSPWCTPGLRFYQTMMEMDRRARLANQDRLRALRRSRGGEIELFCAHDPFEFRRLAG
                     RPMSLPAGK"
     gene            complement(4602..5588)
                     /gene="denD"
                     /locus_tag="VM406_13545"
     CDS             complement(4602..5588)
                     /gene="denD"
                     /locus_tag="VM406_13545"
                     /EC_number="1.1.1.410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythronate dehydrogenase"
                     /protein_id="HVL77033.1"
                     /translation="MEVLITGGAGLLGARLARQLLARGTLADANGRQTAITRITLLDV
                     VPAQGFDDPRIRVVTGDIADEKAIASVLTPDTQSIFHLAAIVSGQAEADFDLGMRINF
                     DATRTILERARRNGNRPRVVFTSSVAVFGGELPARVPDDQLLMPQSSYGAQKVMGELL
                     VNDYSRKGFIDGRALRMPTVCVRPGAPNRAASSFASGIIREPLNGIEAVCPVAAETPM
                     WLLSPRYAIESLVHGHDIDGSLFGASRALSLPGLATTVKEMVEALGRVAGGEAVKRIR
                     WEQDPAVIRIVNSWPGNFDTQRANAMGFGRDADFDSIVRAYIEDELDKKLAA"
     gene            5920..6705
                     /locus_tag="VM406_13550"
     CDS             5920..6705
                     /locus_tag="VM406_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HVL77034.1"
                     /translation="MSNAPLFQVEPLAAPAKMPDRVTQALLELIRGGAYPPKSRLPSE
                     TEMAQHFGVSRTVIREAVSRLKSEGLVESRQGSGVFVREAGPDAPFRIDPGVMDSIQQ
                     VLQVAELRRGLEAEIAALAAERATPQQVRAIGKCLRAIDEDVAAGGDGVAADIEFHRS
                     IARATNNPHFLALWDFLGQFLKGTIKLTRTWEARQEETRRQVLDEHRAVYEAIVNKDV
                     EAARAAARRHMEMASHRIRHADPDFLSARGAPAPRARTRKTGA"
     gene            6733..8001
                     /gene="otnK"
                     /locus_tag="VM406_13555"
     CDS             6733..8001
                     /gene="otnK"
                     /locus_tag="VM406_13555"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HVL77035.1"
                     /translation="MPLLLGCIADDFTGATDLAGMLVRAGMRTVQMIGVPRGPLPDDV
                     DAAVIALKSRTSAPAQAVDESLAALERLREAGCRQFYFKYCSTFDSTDAGNIGPVTDA
                     LMDALGTDFTIACPAFPENKRTIFKGHLFVGDVLLSESGMRDHPLTPMTDANLARVLQ
                     RQTRRKVGLIDHATVAAGAVAIRQRIAALRAEDTGIAIVDAVCNDDLMRIGAACADLP
                     LVTAGSGIALGLPQNFRAAGLLGDGAAADTLAFPQGLRAILSGSCSVATNAQVAHFIE
                     QGGAAYAIDPLRLAAAEDVVGEALEWAQSKLQDGPPLIYATASPDSVKATQARLGVER
                     AGALIEEALAAIAEGLVVRGVRRMLVAGGETSGAVVTALGAHGLRIGPQIDPGVPWTV
                     TLGGEPLALALKSGNFGSTDFFTKAWELLA"
     gene            7998..8636
                     /gene="otnC"
                     /locus_tag="VM406_13560"
     CDS             7998..8636
                     /gene="otnC"
                     /locus_tag="VM406_13560"
                     /EC_number="4.1.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate 4-phosphate decarboxylase"
                     /protein_id="HVL77036.1"
                     /translation="MNESALREQICECGKSLYDRGLATGSSGNISVRTDDGWLLTPTN
                     ACLGKLDPARLSKLDWNGKLISGDAPSKEAFLHRAMYEERGSAGAIVHLHSTHSAAVS
                     CMSGLDPCDCIPPLTAYFVMKIGRLPLVPYHRPGDPKLGDAIRGLAGRHSAVLLANHG
                     PVVSGKSLEAAMYATEELEETAKLFLLLRGHHPRVLDEEQIAELRQVFQLDQ"
     gene            8651..9424
                     /gene="otnI"
                     /locus_tag="VM406_13565"
     CDS             8651..9424
                     /gene="otnI"
                     /locus_tag="VM406_13565"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HVL77037.1"
                     /translation="MPKFAANLTLLFNELPFLDRFEAAAKAGFKGVEYLFPYDYPAEE
                     IAARLKAHGLQNVLFNMPPGDWAGGERGIAALPGREEEFRAGLERAIAYANALGTPRL
                     HAMAGLVPEGADQAQCRATFIANLKTACAEAARHGITVLIEPINTRDMPGYFLSLQAD
                     AHAIREEVGAANLKVQMDFYHVQIMEGDIAMKLRRWLPHIGHIQIAGVPERNEPDTGE
                     IRYPYLFALLDSLGYDGWLGCEYRPAKGTVEGLGWMKNA"
     gene            9511..10485
                     /locus_tag="VM406_13570"
     CDS             9511..10485
                     /locus_tag="VM406_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HVL77038.1"
                     /translation="MRISALALAIAGLSAGFMQTASAQTVMRISVSTAQNSHQGVAID
                     TLAREVEKRTNGRYKIQTFYGGSLGAEREAVEAVQLGTQELTWTSTGPIPNFVPEVKI
                     LDIPFLFRDYAHARAVLDGPIGQDMLKKFESKGMKALAWGENGFRHMTNNRRSIVAPE
                     DMRGLKMRTMENPVHIQAYKGFGIIPTPMAFTEVFTALQQGTVDGQENPLSVIVANKF
                     EQVQKHLTLTGHVYSPGVFLMNKAAFDKLSPADKEAFLEAAKVAAKANRDRVDADERT
                     AVADLRSKGMNVVDNIDKAKYQAALTPVFADFEKQFGKAELDKIRNYK"
     gene            10981..11436
                     /locus_tag="VM406_13575"
     CDS             10981..11436
                     /locus_tag="VM406_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HVL77039.1"
                     /translation="MTSLGIACLLLALAASLGVFQVLMRFVFQQPAEWTEVIIRFSLI
                     WMVFLGIPEAFRVGAMVSIDLVQRVAPPLMRRVLQFIVLIATVTLLGFIIWYGWDYAQ
                     RGSVQTVAGLENVSMFWAYLALPVGSVIALVGIVCNFFEPQQRELDNAQ"
     gene            11452..12729
                     /locus_tag="VM406_13580"
     CDS             11452..12729
                     /locus_tag="VM406_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HVL77040.1"
                     /translation="MTVTMIGTMIVCFALSVSIAVSIGLASIVGIQASNANMLLLAKE
                     IFTSINKFPLAAIPFFILAGTLMESGGISQRLVDFAKSLVGGVQGGLAATCVLTCLIF
                     ASVSGSSVATTFAIGAILVPALIRHGYPRNYAAALQATSAELGVIIPPSIPLILYGVS
                     AEVSIGELFIAGFGPGVLIAAALMAFVIINARMNGYGRNDREGKMPVLVAAKKAWLAL
                     LMPVIILGGIYGGIFTPTEASAVAVCYAFVLGVFIYRETKISDLGKVLKKAVISSAVI
                     MFIIANAGLFSFIITRAGVPAAIGQYLSTVLENPMLFLLGVNAALFVIGMFIETSAAI
                     IVLAPILVPVAIQFGVDPVHFGMIMIINLALGMITPPFGVNLFAACTVAKIPLDNIVS
                     KLIPFVLLIIGCLAIVTYFPPLSLFLRDLVYMR"
     gene            12950..>14107
                     /locus_tag="VM406_13585"
     CDS             12950..>14107
                     /locus_tag="VM406_13585"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVL77041.1"
                     /translation="MSDAAPLQHEAACTTEIVRLTQRIQHLEHAERVQRALFAIADLA
                     GSQLDMREVLRRVHEIVAGLMYAQNLYIARYDAAQDTISFIYFVDVATPAPERAAVPM
                     RALEHSLTWHVIRERRALRGTLDAIADQVGGRLKALGADARDWLGVPMLDGGEVRGVL
                     VVQSYDQADLFTAADQALLEFVASHVLTALERRQARADLESAVQARTAELAAANAELV
                     RQVRLQRSLYRIAELASRADSIEAFCKAVHEEVGELLLAKNFFIALLSEDGRMLHFPY
                     YVNEYDPPSPSRPLGNNVTEYVLRLGSPVLLDQAGIARLAAAGEFAVNGLCPISWLGV
                     PLRCEGRTLGVMTVQTYSGSIRYTQADQDLLQFVSHQIASSLERRRALASLH"
BASE COUNT         2421 a         4718 c         4568 g         2400 t
ORIGIN      
        1 tcgaatacgg gaggatgatc gccagcgtct cccagttcag tggcacgtcc ggcagcagaa
       61 acaccggcag gctgtccggc agctcgccca tgtcgccgac agtacggatg tcgagtccga
      121 atgccatcga tactgcggtc agcaccatga tgcacaccag cggcgacggg actgcctttg
      181 tgatgcgcgg aaacagatag atgatcgcga gtccggcggc agtcatcgcg tagacggtcc
      241 agctgacatt cgtcagctcc ggcaactgcg ccatgaagat caggatcgcg agggcattga
      301 cgaagccgat cacgaccgag cgggagacga agcgcatcag ggagccgagc ttgagcacgc
      361 cggcggcgat ttgcagtaca ccggtgagca gcgtggcggc aagcaggtat tgcagaccgt
      421 gttccttcac cagcgtgacc atcaccaacg ccattgcacc tgtggcggcc gagatcatgc
      481 ccggccggcc gcccgcaaag gcgatcacga cggctatgca gaacgacgcg tacaggccaa
      541 ccttgggatc gacgccggcg atgatggaaa aggcaatcgc ctccgggatg agggcgaggg
      601 cgacaaccag gccggaaagc aggtcgccgc ggacattgga aaaccagtcc tgacggatct
      661 tgttggggag catcttgttc tttcaagttc tggcgcggct tcagggaagc gtgcgcgcga
      721 aacaatgcgc cttcgacgga cgcagaacgg tccaccacat atgaagacgg ctaagacgag
      781 gccggcagag gcctgagtgg atccgacccc cgggcggtga tcagccctgc gggagtcgat
      841 tggaggaggc gtggtcttac atcggacagg tttgcttcag gaaggcgcgc agtttaacag
      901 ccatgctgcg acgcgtcaaa gcaaccggcc cgacgactgc ctacccgaca gcgcgcagta
      961 cgaacatggc aaagctgatg tacattcagc cgacacccgc accacacgca ttcatcgcaa
     1021 tgaccagtcc accaaggtcc gaacccgcca ttcgcaagcc acccgcctcc gagtccctgt
     1081 ccgccctcgc cccgctccag catccggtat tccgcatgct ctggacggtc tggctcgttg
     1141 cgaacatctc gatgtggatg aacgatgtgg ccgccgcctg gatcatgact tcgctgacca
     1201 gcgtgccgat ctgggtggcg ctggtccaga ccgcggcgac gctgccggta ttcctgctcg
     1261 gcctgcccag cggcgcgatg gccgacatct tcgaccggcg gcgctatttc atcgtgaccc
     1321 agttctgggt cgcgatcgtc gcgctcattc tttcggtggt tatcctggca ggcgtgctga
     1381 caccgggtct gctgctggcg ctcacatttg ccaacggcat cggcctggca atgcgctggc
     1441 cggtgttcgc ggcgatcgtt ccggaactgg tgccgcgacc gcaactgccg tccgcgctgg
     1501 ccctgaacgg tgtggcgatg aacctctcgc gcatcgtcgg cccgctgctg gccggtgcgc
     1561 tgatcgccac cgcgggcagc gcctgggtgt tcgtgctcaa tgcggtgctg tcgatcgcct
     1621 gcgcgttcgt gatcatgcgc tggcgtcgcg agcatgtgcc gcacccactc ggacgcgagc
     1681 ggctcacgcg cgccatccgc gtcggcctgc agttcgtcgg tcagtcgacg cggctcaagg
     1741 cggttctgtt gcgcatcttc attttcttct tccattcgac cgcgctgctc gcgctgttgc
     1801 cgctggtcgc gcgcgatctc ggcgcaggcg acgccgcgac gttcacgctg ctgctggcga
     1861 ccatgggcgc cggcgcgatc gccgccgcaa tgctgctgcc gcgcgtgcgg cgcacctgga
     1921 cgcgcgacac cgtcgtcatc ggcggcacca cgctgcagtc ggtctcgatt ctcgtggtgg
     1981 cactggcgcc gaacaccgcc atcgccgtgt tcggcatgtt cgcagcaggc gtggcatgga
     2041 tctcgaccgc caattccctg tccgtctcgg cccagctctc gctgccggac tgggtacggg
     2101 cgcgcggcat gtcgatgtac cagatggcga tcatgggcgg cagtgccgcc ggcgcggccg
     2161 cgtggggaca ggtggcgacg atgaccgatg tcgataccgc gctgatcgcc tccgcagtga
     2221 ccggcgtggc cgcgatgctt gcggcctacc ggatcatcgt cgaccgcagc atcgaggaag
     2281 acctgacacc ctcgcgcgag ttcaaggcgc cggttgccga agcgccgccg ccctcgggca
     2341 acatcgtggt gacgatcgaa tacctgatcg atccgcagcg cagcgcggaa ttccgcgaac
     2401 tgatgcagga gagccggcgc agccgtctgc gccagggtgc gctgtcatgg tcgctgctgc
     2461 gtgatgtgac ggtgccgggg cgcttcgtcg agcagatcgt cgacgaatcc tggaccgagc
     2521 acctgcgccg cttcgaccgc gtgacggccg gcgacgtggc gctgcgcgag cgcaagctcg
     2581 cctttcatgt cggcgataca ccgcccgtga tcacgcgttg cgtggtggag gcaagctgaa
     2641 cgcgccctgc atggcctttc ctcagccctg gggcatggcc tgttcaagtc cggcgaaggc
     2701 cgtgtcattg gtccgctcga actgcagcgg cgtgaccgac acgcgccccc gggcgacagc
     2761 ggcggtttcg ctctccggcg catcctcgta cggctggcgg ttgaactgca gccagtaata
     2821 ggtgaagccg cgcggatcgg tgcgcgcgag cacgtcgata ttcttcacga gccctgcgcc
     2881 ctggcgggtg aacgtgaccg gtccggcctc ttcggcgggc acatccggga agttcacgtt
     2941 caggcaggcg ttcgcattcc atccggcgtc ccataaccgc cggatcacgt ccggggccag
     3001 ggcccgcgca gtgtcccagc gcaccgcctg gcggtcggta aaggcctggc tcagcgcaat
     3061 cgaaggcagt ccgagcagca ggccggtcat cgccgcgccg acggtgccgg agaacacggt
     3121 ctccacgccg aggttcgcgc cccggttgat gcctgatagc accagcgagg gccgcgcgtc
     3181 cgccatgatg tgctgcaagg ccatcaccac gcagtctccc ggcgtgccag agacgctgta
     3241 gcgccggttg ccgagatcgt gcacgcgcaa cggcgcatgc aggctgatcg agtgcgacgt
     3301 gccgctgcgg tcatgttcgg gcgcaaccac ccagacctcg tcggcaatcg aagcggccac
     3361 ctcttcgagc gcgcgcaagc ccggcgcgtc tatcccgtcg tcgttggtca gcaggacccg
     3421 ctcgattgct cgcttctgca tcattgatca gcctcctggt tgccatcgca cgcccgcagc
     3481 aacgatgccg cgaaaaaaca gtttagaggc gagggcacag gcggagttcg gcgggcggtt
     3541 tcacttgccg gcaggcaggg acatcggacg gccggccagc cggcgaaact cgaacgggtc
     3601 gtgcgcgcag aacagttcga tctccccgcc gcgcgagcgg cgcagcgcac gcagccgatc
     3661 ctggttcgcc agccgggcgc gccggtccat ttccatcatc gtctggtaaa aacgcaggcc
     3721 cggggtgcac cacggcgacg ataaattcat ctcgcggtgg aagaaatagg catcgccggc
     3781 ctgcagcagc caacggtcgg cctggcgaac cgcaataccg gcatgcccat aggtatgacc
     3841 gggcagcggc accatgagga tctccggcgg aacaccggcg aggtcgcgca cgcattcgaa
     3901 gccgtaccac ccttcccccc ggccgagccg gtagaccttc cagcgcttgc ggctcgacca
     3961 ttgctgcggg cgatagcgct ggcggtccag ccacgaccgt tgtgccagcg catcgtcgcg
     4021 ttcctgctgc agcatgtgca ccgttgcttc aggaaaatcg tcgagcccgc ccgcgtggtc
     4081 gaaatcgaga tgggtgagca cgatatggcg cacgtccgtg gcggaaaatc ccagcgcctc
     4141 gatctgtcgc accgccgtca tctcctcgcg aaaatccggg cttagcagcg caaggaagaa
     4201 atggctcaga cgtccgcgcg gatcggcgac atcgcgcagg ccgaagccgg tatcgatcag
     4261 caccagcccc gcttcggtct cgaccaagag gcagtggcag cacagtccgc cgcgcccgaa
     4321 aacagtggaa gtctgcccgt ccatcagttt gccgccgagc gggcaggacg agatgcaatt
     4381 caggtgatgc acacgcatgc gattctccgg agcaaacggc aacagggaca ggccgctcgc
     4441 acctgccgga ctgcttcgga gcttgcgcct cacccatccg cctgaccgga tgtgctgtca
     4501 ttcggttccg tgtcctgctg cctgcttccg ctcggcatcg cttgctgtcg ctgttctgcg
     4561 gcagctgcgc agcccgccgc tcgaaccgcg gaatcgcttc gtcaagcggc gagcttcttg
     4621 tcgagctcgt cctcgatgta ggcgcgcacg atgctgtcga agtcggcatc acggccgaag
     4681 cccatcgcat tggcgcgctg cgtatcgaag ttgccgggcc aggagttgac gatgcggatc
     4741 acggcggggt cctgctccca gcggatacgc ttgacggctt cgccgccggc cacgcgtccg
     4801 agcgcctcca ccatctcctt tacggtcgtc gccaggcccg gcaggctcag cgcgcggctg
     4861 gcaccgaaca ggctgccgtc gatgtcgtga ccgtgaacca ggctttcaat cgcgtagcgc
     4921 ggcgagagca gccacatcgg cgtctccgcc gccaccgggc acaccgcctc gatgccgttg
     4981 agcggttcac ggatgatgcc gctggcaaaa ctcgacgccg cccggttcgg cgcgccggga
     5041 cgcacgcata cggtgggcat gcgcagtgcg cggccgtcga tgaagccctt gcggctgtag
     5101 tcgtttacca gcagctcgcc catcaccttc tgcgcgccgt atgaactttg cggcatcagc
     5161 aactggtcgt ccggcacgcg cgccggcagc tcgccgccga atacggcgac cgagctggtg
     5221 aacaccacgc gcgggcgatt gccgttgcgg cgcgcgcgct caaggatggt gcgggttgca
     5281 tcgaagttga tacgcatgcc gagatcgaag tccgcttcgg cctggccgct gacgatggct
     5341 gcgaggtgga agatcgactg cgtgtccggc gtcagcacgg atgcaattgc cttctcgtcg
     5401 gcgatgtcgc cggtgaccac gcgtatgcgc ggatcgtcga agccctgcgc cggcaccacg
     5461 tcgagcaatg tgatgcgcgt gatcgcggtc tgtcggccgt tggcgtcggc gagcgtgccg
     5521 cgcgcgagaa gctggcgcgc cagacgcgcg cccagcagtc cggccccgcc ggtgatcagt
     5581 acttccattc ttcttcctct cttcatgctt gcgcggcgcg caagtcggca tggtcaaact
     5641 cgatcacgcg ctgtttccga gtgcgtgaat aattcttgcg ggtgccggag cgcagcttgc
     5701 gatgcgacga cactccctta ccgtgccacc cctcactctg tcgctcccgc gcaggcggga
     5761 gcccagctac atactttcca ccggcgtcag cgcatctccc gggttcccgc gcgggagggg
     5821 cgccgtcttc acaacaaccc aaacccggtt gtgacttccc gaaccaagtt gtatgatgag
     5881 catatatcat atctccatat aagccagacg gatatctcag tgagcaacgc gccgctcttc
     5941 caagttgaac cgctcgccgc gccggccaag atgccggacc gggtcacgca ggccttgctg
     6001 gagctgattc gcggcggcgc ctacccgccc aaatcgcgcc tgccgtcaga gacggagatg
     6061 gcccagcatt tcggcgtcag ccgcaccgtg atccgcgaag cggtatcgcg cctgaaatcg
     6121 gaaggtctgg tcgaatcgcg ccagggcagc ggcgtgttcg tgcgcgaggc ggggccggat
     6181 gcgccgtttc gcatcgatcc cggggtgatg gattcgatcc agcaagtgct ccaggtggcg
     6241 gagctgcggc gcggactgga agcggagatc gcagcgctcg cggccgagcg ggcgaccccg
     6301 cagcaggtgc gcgcaattgg caaatgcctg cgcgcgatcg acgaagacgt ggcggccggc
     6361 ggcgacggcg tggcggcgga tatcgagttc catcgcagca tcgcacgcgc gaccaacaac
     6421 ccgcacttcc ttgcgctgtg ggattttctc ggccagttcc tgaagggcac gatcaagctc
     6481 acgcgcacct gggaagcccg gcaggaagaa acgcgccggc aggtgctcga cgaacaccgc
     6541 gcggtctacg aggcgatcgt gaacaaggac gtggaagcgg cgcgggcggc ggcgcgcagg
     6601 catatggaaa tggcgtcgca ccgtatccgt catgccgacc ccgatttcct tagcgcgcgc
     6661 ggcgctccgg ctccgcgggc tcgcaccaga aaaaccggcg cctgaagcgc tgcaagaaag
     6721 aacggattac ccatgcccct gctgctcggc tgcatcgctg acgatttcac cggagccacc
     6781 gacctggccg gcatgctggt tcgcgccggc atgcgcaccg tgcagatgat cggcgtgccg
     6841 cggggcccgc tgccggatga tgtcgatgcc gcggtgatcg cattgaagtc gcgcacctct
     6901 gcccccgccc aggcggtcga cgaatcgctg gccgcgctgg agcgcctgcg cgaggccggc
     6961 tgccgccagt tctatttcaa gtactgctcg accttcgatt ccaccgacgc cggcaatatc
     7021 ggcccggtca ccgatgcgct gatggatgcc ctcggcaccg acttcacgat cgcgtgcccg
     7081 gccttccccg aaaacaagcg cacgatcttc aagggccatc tgttcgtcgg cgacgtgctg
     7141 ctctcggaga gcggcatgcg cgaccatccg ctcacaccaa tgaccgatgc gaacctcgcg
     7201 cgcgtgctgc agcggcagac gcgtcgcaag gtcgggctga tcgaccatgc aacggtggca
     7261 gcgggcgccg tcgcgatccg gcaacgcatc gcggcgctgc gcgccgagga caccggcatc
     7321 gcgattgtcg atgcggtttg caacgacgac ctgatgcgca tcggcgcggc ctgcgccgat
     7381 ctgccgctgg tgacggccgg ctccggcatc gcgctcggcc tgccgcagaa ctttcgcgcc
     7441 gccggcctgc tgggcgacgg cgccgccgcc gacaccctcg ccttccccca gggtttgcgc
     7501 gcgattcttt caggcagctg ctcggtcgcg accaatgcgc aggtcgcgca cttcatcgag
     7561 caaggcggtg ccgcctatgc gatcgacccg ctgcgcctgg ccgcggccga ggatgtggtc
     7621 ggcgaagcgc tcgaatgggc gcaatcgaag ctgcaggacg gcccgccgct gatctacgcg
     7681 acggccagcc ccgacagcgt gaaggcgacc caggcccggc tcggcgtcga gcgcgccgga
     7741 gcgctgatcg aagaggcact ggcggcgatc gccgaaggcc tggttgtgcg cggcgtgcga
     7801 cggatgctgg tggccggcgg cgaaacctcg ggcgcggtgg tcaccgcgct cggcgcgcac
     7861 ggcctgcgca tcggcccgca gatcgacccc ggcgtgccgt ggaccgtgac cctgggcggc
     7921 gagccgctgg cgctcgcgct caagtccggc aacttcggca gcaccgactt cttcaccaag
     7981 gcctgggagc tgctggcatg aacgaatccg cattgcgcga acagatctgc gaatgcggca
     8041 agtcgctgta tgaccgtgga ctggccactg gcagcagcgg caacatcagc gtgcgcaccg
     8101 acgacggctg gctgctcacg ccgaccaacg cctgcctcgg caagctcgat ccggcccggc
     8161 tgtccaagct cgactggaac ggcaagctga tctccggcga cgcgccttcg aaggaagcct
     8221 tcctgcaccg cgcgatgtac gaggaacgcg gcagcgcggg cgcgatcgtg cacctgcatt
     8281 cgacgcactc ggccgccgtg tcctgcatgt ccggcctgga cccgtgcgac tgcattccgc
     8341 cgctgaccgc ctacttcgtg atgaagatcg gccgcctgcc gctggtgccc taccatcgcc
     8401 ccggtgaccc gaagctgggc gatgcgatcc gcgggctcgc gggcaggcac agcgcggtgc
     8461 tgctcgccaa tcatggcccg gtggtgtccg gcaagtcgct ggaagcggcg atgtatgcga
     8521 cagaggaact ggaggaaacc gcgaagctgt tcctgctgct gcgcggccat catccgcgcg
     8581 tgctggacga ggaacagatc gccgagctgc ggcaggtttt ccagctggat caatgaagaa
     8641 aggaccgtcc gtgccgaagt tcgccgccaa tctcacgctg ctgttcaacg aactgccctt
     8701 cctcgaccgc ttcgaggcgg cggcgaaggc cggcttcaag ggcgtcgagt acctgttccc
     8761 ctacgactac ccggccgagg agattgccgc aaggctgaaa gcacatggct tgcagaacgt
     8821 gctgttcaac atgccgcccg gcgactgggc gggcggcgag cgcggcatcg cggcgctgcc
     8881 cgggcgcgaa gaggaattcc gcgccggact ggagcgggcg atcgcctatg cgaacgcgct
     8941 cggcaccccg cgcctgcatg cgatggccgg cctggtgccg gaaggcgcgg accaagcgca
     9001 atgccgcgcc accttcatcg ccaatctgaa gaccgcctgc gcggaagccg ccaggcatgg
     9061 catcacggtg ctgatcgagc cgatcaacac gcgcgacatg cccggctatt tcctgagcct
     9121 gcaggccgac gcccacgcga tccgcgagga agtcggcgcc gccaacctga aggtgcagat
     9181 ggacttctac catgtgcaga tcatggaagg cgacatcgcg atgaaactgc ggcgctggct
     9241 tccgcatatc ggccacattc agattgccgg cgtgcccgag cgcaatgagc ccgacaccgg
     9301 cgagatccgc tacccctacc tgttcgcgct gctcgacagc ctcggctacg acggctggct
     9361 cggctgcgaa taccgcccgg ccaagggaac ggtcgaggga ctgggctgga tgaagaacgc
     9421 ttgatttacc cccgagcagt gccaaaccgc tgcgcgagca gcagtcaaca accccgttgg
     9481 aaaaaactga ggagaccaac aatgaaaacc atccgcatca gtgcccttgc tcttgcaatc
     9541 gccgggctgt ccgccggctt catgcagacg gcgtccgcgc agactgtgat gcgcatctcg
     9601 gtatcgaccg cgcagaactc ccaccagggc gtggcgatcg acacgctcgc gcgtgaagtg
     9661 gaaaagcgca ccaacggccg ctacaagatc cagaccttct acggcggatc gctcggcgcc
     9721 gagcgcgagg cggtcgaagc ggtgcagctg ggcacccagg agctgacctg gacctcgacg
     9781 ggcccgatcc cgaacttcgt cccggaagtg aagatcctcg acattccgtt cctgttccgt
     9841 gactacgccc acgcgcgcgc cgtgctggac ggcccgatcg gccaggacat gctgaagaag
     9901 ttcgagtcca agggcatgaa ggcgctggcc tggggcgaga acggtttccg ccacatgacc
     9961 aacaaccgcc gctctatcgt cgcgccggaa gacatgcgcg gcctgaagat gcgcacgatg
    10021 gaaaacccgg tgcacatcca ggcctacaag ggcttcggca tcatcccgac gccgatggcc
    10081 ttcaccgaag tgttcaccgc gctgcagcag ggcacggtcg acggccagga gaacccgttg
    10141 tcggtcatcg tcgccaacaa gttcgagcaa gtgcagaagc acctgacgct gaccggccac
    10201 gtgtactccc cgggcgtgtt cctgatgaac aaggccgcgt tcgacaagct ctcgccggcg
    10261 gacaaggaag ccttcctcga agccgcaaag gtcgccgcca aggccaaccg cgaccgcgtg
    10321 gatgccgacg agcgcaccgc cgtggcggac ctgcgttcga agggcatgaa cgtggtggac
    10381 aacatcgaca aggccaagta tcaggctgcg ctgacgccgg tgtttgcgga cttcgagaag
    10441 cagttcggca aggccgagct ggacaagatc cgcaattaca aataagggtg aaaggggtgc
    10501 ggtgcgccct tgcccttgcc ttttcaaatg ccacggaaag accccttccc ccttccaggg
    10561 ggatgagagc ggggtttcgc agagcatgct ctgcgccgct cgaatccgag cagcttgcaa
    10621 gctgcgaggt ggaaggttgg gatggggcgg cccgcgtagg ggtgcggagt ccgatcaggc
    10681 gacgacgcta gctcgggctc cgcaccccct ccctagcccg tccacgtcgc cgcttgcaag
    10741 cggctccgat tcggtcggcg caaagcggtg ctttgcgaaa ccccgacctc atccccctgg
    10801 aagggggagg ggaccgtgca gcgcgtgagc acggcgcccc acgattcatg tgaacttgat
    10861 acgcacgcga taacacgctg acttccgccc actccaacgc ccacgaccat gcagcctgcc
    10921 tacacggtta aacaaagctc cacctcatcg gccctgctcg ccatcggccg cggcatcacg
    10981 atgacttcgc tcggcatcgc ctgcctgctg cttgcgctgg cggcctcgct cggggtgttc
    11041 caggtcctga tgcgcttcgt gttccagcag ccggccgagt ggaccgaggt gatcatccgc
    11101 ttctcgctga tctggatggt cttcctaggc attcccgagg ctttccgcgt cggcgcgatg
    11161 gtgtcgatcg acctcgtcca gcgcgtcgcg ccgccgctta tgcgccgcgt gctgcagttc
    11221 atcgtgctga tcgccaccgt cacgctgctc ggcttcatca tctggtacgg ctgggattac
    11281 gcgcagcgcg gcagcgtgca gacggtggca ggactggaga acgtgtcgat gttctgggcc
    11341 tacctggcgc tgccggtcgg ctcggtcatc gccctcgtcg gcatcgtctg caatttcttc
    11401 gagccgcagc agcgcgagct cgacaacgcc caataagaac agagggacat catgactgta
    11461 acgatgatcg gcacgatgat cgtgtgcttc gcgctgtcgg tatcgatcgc ggtatcgatc
    11521 ggcctggcca gcatcgtcgg catccaggcc tcgaacgcga acatgctgct gctggccaag
    11581 gagatcttca cctcgatcaa caagtttccg ctggccgcga tcccgttctt catcctcgcc
    11641 ggcacgctga tggagtccgg cggcatctcg caacgcctgg tcgatttcgc caaatcgctg
    11701 gtcggcggcg tacagggcgg gctggcggcc acctgcgtgt tgacctgcct gatcttcgcc
    11761 tccgtgtccg gctcctcggt cgccaccacc ttcgcgatcg gcgcgatcct ggtgccggcc
    11821 ctgatccggc acggctatcc gcgcaactac gcggccgcgc tgcaggcgac atcggcggaa
    11881 ctgggcgtga tcattccgcc gtcgattccg ctgatcctgt acggcgtgtc ggccgaagtg
    11941 tcgatcggcg agctgttcat cgccggcttc ggtcccggcg tgctgatcgc cgcggcactg
    12001 atggccttcg tgatcatcaa tgcgcggatg aacggctacg ggcgcaacga ccgcgagggc
    12061 aagatgccgg tgctggtggc cgcgaagaag gcctggctgg cgctcctgat gccggtgatc
    12121 atcctgggcg gcatatacgg cggcatcttc acgccgaccg aagcctcggc ggttgccgtc
    12181 tgctacgcgt tcgtcctcgg cgtattcatc taccgggaaa cgaagatctc cgacctgggc
    12241 aaggtgctga agaaggccgt gatctcgtcg gccgtgatca tgttcatcat cgccaatgcc
    12301 ggcctgttca gcttcatcat cacgcgcgcc ggcgtgccgg cggcgatcgg ccagtatctc
    12361 tctacggtgc tggaaaaccc gatgctgttc ctgctcggcg tgaatgccgc gctgttcgtg
    12421 atcggcatgt tcatcgaaac ctcggcggcg atcatcgtgc tcgcgcccat cctggtgccg
    12481 gtggcaatcc agttcggcgt cgacccggtg cacttcggca tgatcatgat catcaacctc
    12541 gcgctcggca tgatcacgcc gccgttcggc gtgaacctgt tcgccgcctg cacggtcgcg
    12601 aagattccgc tggacaacat cgtgtccaag ctgatcccgt tcgtgctgct gatcatcggc
    12661 tgcctggcga tcgtgaccta cttcccgccg ctgtcgctgt tcctgcgcga tctggtctac
    12721 atgcgttgac gctgccgcaa tgcatgggtc cccggggctt gtccacggct accgcattcg
    12781 cgacaggctc tgcacgtaag cggcgcgccg ggacctgact gaacggctcg gccgcgcgtc
    12841 cgggccggcg tgagtggcac ggacgcgacg ctgccctcat gcgcagatgc gcagcgacct
    12901 tggaatcggt agactggcag gaacttactt gcccaacgga aatttcgact tgagcgacgc
    12961 cgcgcccctc cagcacgagg cagcctgcac aaccgagatt gttcggctca cgcaaaggat
    13021 ccagcacctc gaacacgcgg aacgcgtcca gcgcgcgctg ttcgccattg cggacctcgc
    13081 cggctcccag ctcgacatgc gggaagtgct gcgacgcgtg catgaaatcg tcgctgggct
    13141 catgtacgcg cagaacttgt acatagcgcg ctatgacgcg gcacaggaca cgatcagctt
    13201 catctatttc gtcgatgtgg ccacacccgc accggagcgc gccgcggtcc cgatgagggc
    13261 gctggagcac agcctcacct ggcatgtgat ccgcgaacgg agggcgctgc gcggcacgct
    13321 ggacgcgatt gccgatcagg tcggcggacg gctcaaggct cttggtgccg atgcacgaga
    13381 ctggctgggc gtgccgatgc tcgatggcgg cgaggtgcgc ggcgtgctcg tcgtgcagag
    13441 ctacgaccag gccgatctgt tcaccgctgc cgaccaggcc ctgctcgagt tcgtcgcgag
    13501 ccatgtactg actgcgctgg aacgccgcca ggcgcgcgcc gatctcgaaa gcgcagtgca
    13561 ggcgcgcacc gccgagctgg ccgcagccaa tgccgagctg gtgcgccagg tgcgcctgca
    13621 gcgctcgctt taccggatcg ccgaactggc gagccgggcg gacagcatcg aagcgttctg
    13681 caaggccgtc catgaggagg tgggcgagct gctgctggcg aaaaacttct tcatcgccct
    13741 gctctccgaa gacgggcgca tgctgcactt cccgtactac gtcaacgaat acgatccacc
    13801 cagcccgagc cgcccgctcg gcaacaacgt gaccgaatac gtgttgcggc tcggttcccc
    13861 ggtcctgctc gaccaggccg gcatcgcgcg cctggccgcc gcaggtgaat tcgcggtcaa
    13921 cggcctgtgc ccgatctcat ggctcggggt gccgctgcgc tgtgaaggcc gcacgctggg
    13981 cgtgatgacg gtgcagacat attccgggtc gatccgctac acgcaagcgg accaggacct
    14041 gctgcagttc gtatcgcatc agatcgccag cagtctcgag cggcgacgtg cgctggcctc
    14101 gctgcat
//
LOCUS       DATLGF010000445         2765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_215903, whole genome shotgun sequence.
ACCESSION   DATLGF010000445 DATLGF010000000
VERSION     DATLGF010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215903"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VM406_13590"
     CDS             complement(<1..874)
                     /locus_tag="VM406_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVL77042.1"
                     /translation="MSTPNLMRLLLLGAIWGTSFMLMRIAVPDLGPLMTTCLRALLAA
                     AALLLFARASNIRFGWRENGRTYLVIGLFNTAIPFALFAWSALHIPSAYMATMNSLAP
                     IFTGLFGILLLSEKLTAQRMAACVLGMSGVAVLVGVGPTDVSLHVVLGVLASMGAAVC
                     YGFAATYTRMKATRLPSMAVATGSQLAAALVLLPLALIDLPHALAAASLQALVAAVVL
                     GVICTGLAYALFFHLIATEGANKAITVTFLVPATASLWAWLFLDEPITVGTVAGILTV
                     LAATALALGLVGRAG"
     gene            1034..1924
                     /locus_tag="VM406_13595"
     CDS             1034..1924
                     /locus_tag="VM406_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HVL77043.1"
                     /translation="MHFTTLLYEKDANNPRIARITLNRPERLNAINEAMPREIRAAVD
                     AANDDDEVHVIVLQGAGRAFCAGYDLKHFAETSGPDSVIQEDMPWDPMRDYRLMKGYT
                     EDFMSLWRSYKPTICRVQQYAVAGGSDIALCCDFVVMEEGARIGYMPTRVWGCPTTAM
                     WVYRLGPMRSKRMLLTGDTIDGRTAKEWGLVADAVPPGELDTAVNALAERMAGVPKNQ
                     LMMQKLMINQAYNTMGIENTQMVATLFDGITRHSPEGMWFKAYAEQNGFAEAVKWRDG
                     GRPIPEGEEAKAAVRSMKRG"
     gene            2188..2397
                     /locus_tag="VM406_13600"
     CDS             2188..2397
                     /locus_tag="VM406_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77044.1"
                     /translation="MRRRLLQLSVDAGAKEPERLADALVLLIDGALANSHVLGTQGPN
                     LELEHAGAALIDAYLDSGAGADEQG"
     gene            complement(2405..>2765)
                     /locus_tag="VM406_13605"
     CDS             complement(2405..>2765)
                     /locus_tag="VM406_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HVL77045.1"
                     /translation="PELKGLEPGLRGFHVHEKPSCQPAEDEGQVKAAQAAGGHYDPHG
                     AGRHGTPWGDGHLGDLPALYVSPDGGATHPVLAPRLRMENVTGRALMVHAGGDNYADH
                     PEPMGGGGERVACGVIR"
BASE COUNT          518 a          903 c          940 g          404 t
ORIGIN      
        1 ggccggcacg cccgaccagc ccgagcgcca gagcggtggc ggccagcacg gtcaggatgc
       61 cggcgaccgt gcccacagtg atcggctcgt cgaggaagag ccaggcccac agcgaggcgg
      121 tggcgggcac caggaaggtc acggtgatcg ccttgttggc gccttccgtt gcgatcaggt
      181 ggaagaacag cgcataggcg agaccggtgc agatcacgcc gaggacgacg gccgcgacca
      241 gtgcctgcaa actcgccgcg gccagcgcat gcggcaggtc gatcagcgcc agcggcagca
      301 gcacgagcgc ggcggcgagc tggctgccgg tcgccaccgc catcgagggc agccgggtcg
      361 ccttcatccg ggtataggtg gcggcaaagc cgtagcagac cgccgcgccc atgctggcca
      421 gcacgccgag caccacatgc aggctcacat cggtcgggcc gacgccgacc agcaccgcca
      481 cgccggacat gccgagcacg caggccgcca tgcgctgcgc ggtcagcttt tcagagagca
      541 gcaggatgcc gaacaggccg gtgaagatcg gcgcgagcga attcatcgtc gccatgtagg
      601 cagacggaat gtgcagcgcc gaccacgcga acagcgcgaa cggaattgcg gtgttgaaca
      661 ggccgatgac caggtaggtg cggccgttct cgcgccagcc gaagcggatg ttgctcgcgc
      721 gcgcgaacag cagcaaggcc gcggccgcaa gcagcgcgcg caggcaggtg gtcatcagcg
      781 gcccgaggtc gggcacggcg atgcgcatga gcatgaaact ggtgccccag atcgcgccaa
      841 gcagcaacag tctcatcagg ttcggggtgg acacggtcgg ctcctttttc gaatgcgcag
      901 tgtaggtccg ccagcgccag ttgacgaacg ataaatattg gcatcggttg tcaatctgct
      961 tcacaagcgg ggcgattcct tgctaccgta gacgatagtg tgaatatgaa ttcaccgccc
     1021 acgaggagac gccatgcact tcaccactct gctgtatgaa aaggatgcaa acaatccgcg
     1081 gattgctcgc attaccctga accgccccga gcgcctgaac gcgatcaacg aggcgatgcc
     1141 gcgcgagatc cgcgcggcgg tcgacgcggc caacgatgac gacgaggtcc acgtgatcgt
     1201 gctacagggc gcgggccgcg cgttctgcgc cggctacgac ctcaagcatt tcgccgagac
     1261 gagcggtccc gactcggtga tccaggaaga catgccctgg gacccgatgc gcgattaccg
     1321 cttgatgaag ggctacaccg aggatttcat gtcgctgtgg cgctcctaca agccgaccat
     1381 ctgccgcgtg cagcagtacg cggtggccgg cggctccgac attgcgctgt gctgcgactt
     1441 cgtcgtgatg gaagagggcg cgcgcatcgg ctacatgccc acgcgcgtgt ggggctgccc
     1501 gacgaccgcg atgtgggtgt accggctcgg cccgatgcgc agcaagcgca tgttgctgac
     1561 cggcgacacg atagacgggc gcaccgcgaa ggaatggggc ctggtggccg acgcggtgcc
     1621 acccggcgaa ctcgacactg ccgtcaatgc gctggccgag cgcatggcgg gcgtgccgaa
     1681 gaaccagctg atgatgcaga agctgatgat caaccaggcg tacaacacga tgggcatcga
     1741 aaacacccag atggtcgcga ccctgttcga cggcatcacg cggcacagcc cggaaggcat
     1801 gtggttcaag gcctatgcgg aacagaacgg ctttgccgaa gcggtgaaat ggcgcgatgg
     1861 cgggcggccg attccggagg gcgaggaagc gaaggcggcg gtgcggtcga tgaagagggg
     1921 gtgatggcgg cccgaagcgg gctgcggcag atatcaccca tggcactgat ggctcgtcat
     1981 tccgtggtcg ccccggcgca agccggggcc gcacgactct agcgccacgc gctgagcgcc
     2041 gctggtccgg gctttcgccg gaaaaccgat ccctcggatg cgcatcggaa cgccatgaca
     2101 ccagtggcgc cgccacgcgc tccttcagca agtccctgtc cttgccgagc acctccatgc
     2161 cgcgcctgcc atcagcacaa gtccgagttg cgccggcgcc tgcttcagct gtcggtggat
     2221 gccggcgcga aggaacctga acggctggcc gatgcgctgg tgttgctgat cgacggcgcg
     2281 ctggccaaca gccacgtgct cggcacgcaa gggccgaatc tcgaactgga gcatgccggc
     2341 gccgcgttga tcgacgcgta tctggactcg ggcgcgggcg ctgatgaaca gggttgatgc
     2401 gaacctaacg aatcaccccg cacgccaccc gctcgccgcc gccgcccatc ggctccggat
     2461 gatcggcgta attgtcgccg ccggcatgca ccatcaatgc gcgtccggtg acgttttcca
     2521 tcctcagacg cggcgcgagc accggatgcg tggcgccgcc gtccggactc acgtacagcg
     2581 ccggcaggtc gccgagatgg ccgtcgcccc acggagtgcc gtggcggccg gcgccgtggg
     2641 gatcgtaatg cccgcccgca gcctgggccg ccttgacctg cccctcgtct tcagccggct
     2701 ggcagctcgg cttctcatgg acatggaagc cgcgcaaacc cggctccagc cccttcagtt
     2761 caggc
//
LOCUS       DATLGF010000446        11424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_105306, whole genome shotgun sequence.
ACCESSION   DATLGF010000446 DATLGF010000000
VERSION     DATLGF010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 11424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11424
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105306"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1065)
                     /locus_tag="VM406_13610"
     CDS             complement(169..1065)
                     /locus_tag="VM406_13610"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HVL77046.1"
                     /translation="MNAITDLKAMPIEVPPHRPVLNEAQLNEYRETGFLTLRGIFGQD
                     LLQRLTEATERLYKEGSALQAKTRHFDLEEAHRPDNPRIRRISSPTELDPAFIEVAFH
                     SVLGDIAADLVGGPVKFYHSKVNFKLPNGGAEIGWHQDWPVFPHTNTNLVALSVPLNP
                     SRSGNGCLQTIPGSHKQGPRSHWADGKYMLNCNHTMREADFALSEDSELDPGDIVAHH
                     GLVVHGSGANLSDQIRTTYIIQYAAADAFAYTAPVIDSCHRNRMVRGEPARFARVEAG
                     VIELPPDFSNGYSSIYSLQEKR"
     gene            complement(1062..1883)
                     /locus_tag="VM406_13615"
     CDS             complement(1062..1883)
                     /locus_tag="VM406_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HVL77047.1"
                     /translation="MRLTKDQLETYERDGFLVMPGLFSMQEVDAMKAELARIQQIDTD
                     HLVRERSGGVAKTIYRVHEDNGPTASPVFHAASRCPRMLEPAQDVLGDDALYIYHTKC
                     NLKTAIDGSVWQWHQDYGTWRKDGVPEPNLSTALIMLDEPTQLNGCLYFIPGSHKEGN
                     LEPQFDEATGYKFWVVPKQQMLSIMERSPEPVPITGKPGTVVFFHPNILHASGHNLSQ
                     DDRWAIYIVYNPVVNRPHEVPNPRPDYVRSTNFSPLAAGPDDILGRQAAVSEVTA"
     gene            2042..2935
                     /locus_tag="VM406_13620"
     CDS             2042..2935
                     /locus_tag="VM406_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008345399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVL77048.1"
                     /translation="MDMRHLRYFIAVAEELHFTRAASRLHIAQPPLSRQIRELEEELG
                     VTLLHRTKRRIELTDAGQVFLDKARQVLAAADSAIVETQRAQRGQTGKIAVGFFEQTA
                     YTLLPPILHAFQKRFPEVDVQLRWYPVVEQVKALQRGDTDIAFVRPAVDLGEVSTELL
                     LKEPFVLAVPRDHRFAQQEVVSLKECAGERLINYTQTLAPDYCAAITQACAAAGIVPG
                     DFLEVGQVYTALGLVSAGVGLAFVPASVQRVRVDNVVYRPLREKRLQSQAYLAWTRPH
                     PSALLTSFVAVAKRVVAEKFE"
     gene            3044..3460
                     /locus_tag="VM406_13625"
     CDS             3044..3460
                     /locus_tag="VM406_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HVL77049.1"
                     /translation="MNAPQEARLKVAETEKITINLGPVDLGHIDLLVQEGFYSNRTDL
                     IRTAIRNQLQVHADVIRQTVARKRLVLGLQHFTRSELEAARDAGTKLQIQVLGLASIA
                     TDVTPELAVAAIDSITVLGVLQASPTVKAALAGRIH"
     gene            3469..4695
                     /locus_tag="VM406_13630"
     CDS             3469..4695
                     /locus_tag="VM406_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HVL77050.1"
                     /translation="MKLNERFLAQMREATILLESEGPMAATAAIQRALNAHSGPAGRP
                     NQPAQGGRGAEPAEDATPLPSTSDAGAARTDAWFDSIRRQAADAVQDVEFREMESTES
                     GSVGSDSADSAGSGRFLSGACSNSAGTREYKLYVPRGYVGQPLPLIVMLHGCTQDPDD
                     FAAGTGMNILADEQNFLVVYPAQSKNANTSNCWNWFQPTHQARDRGEPSLIADITRKV
                     VREYQVDERRIYVAGLSAGGAMAAVLGARYPDLYAAIGVHSGLPLGVARDVVSAFAAM
                     KGGKGKASSRAATAHPPATLGHTIPVIVFHGDRDRTVHGSNGDKVLEQCIAAGDPACA
                     KANVEVGMARGRRYTRAVHRDAQGRAYAEKWTVHGAGHAWAGGSKKGSYTDPGAPDAS
                     REMVRFFLSHALPGSD"
     gene            complement(4845..5090)
                     /locus_tag="VM406_13635"
     CDS             complement(4845..5090)
                     /locus_tag="VM406_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77051.1"
                     /translation="MTYEEFLDEVATLLTELYDLADEEAIKMVMRAQAADFFTLHDDL
                     PEMRTQERAVQDARTIFRQQKKSKPQVPPKRATKPKK"
     gene            complement(5187..6596)
                     /locus_tag="VM406_13640"
     CDS             complement(5187..6596)
                     /locus_tag="VM406_13640"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HVL77052.1"
                     /translation="MFVALAAIAVAIYAGIQWAYAGKRASLITIALFAVTMVPVFFAV
                     LRMFFMRLPPPQGRRIGREEAPKLFEVLDKMRRKLKGPPIHHVLIDDAYNAAIAQIPR
                     WGMFGGHTSYLILGLPYLLGVPPKEMLATVAHEYGHLCGNHGKLSSWVYRQRRTFGAL
                     YEQVDASREDNWVHGLLAMALDRFMPYYNAYTFVLSRQNEYEADLTATDLVGAEANAS
                     GLIRDALLGRWIHEQFWSTIFKQANTIPRPAIMPFASMRTAFKASYEQWATRERLMQA
                     WREQSGLHDTHPSLRDRVEATGHEAELPAPLEVTAAEALLGAATARRLIVEFDQTWWK
                     AEQKEWEARYRYVQRSQKRLSELAQHQLDALALHDLQELALLKAEFESPLAAKPALEH
                     LLKKPGGPFPKASYYYGRILLGEDDDRGLEYLATAARADRSLTERAAEAGYYYERDKR
                     GEEAARVWWESVVPQEEAA"
     gene            7513..8157
                     /locus_tag="VM406_13645"
     CDS             7513..8157
                     /locus_tag="VM406_13645"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HVL77053.1"
                     /translation="MNTARTVQTVLAAAVVGLCGSSVHAQEFSTQLSGFDEVPLAIFT
                     AGSGTLDLTLDQQAGTVNYTLSYSDLSTPVTQAHIHFGKERVAGGVIAFLCSNLTESP
                     PGTPACPEISGTVSGSITADGVVGIEPQNVEAGDFSALVAALTSGTSYVNVHTKKFPA
                     GELRGEIRGEVPDQSPGDGPGQSPGQSPGQSPGQSPGQSPGQTPSPATPGTPYN"
     gene            complement(8516..8761)
                     /locus_tag="VM406_13650"
     CDS             complement(8516..8761)
                     /locus_tag="VM406_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77054.1"
                     /translation="MSVFTKPVFDGTVIFDGQELFKGQGAAKQWAQQVGNELGIPVEA
                     RKIGTGWALVGTIDGVETVWGVYGQRLKRISASTDED"
     gene            complement(8822..8914)
                     /locus_tag="VM406_13655"
                     /pseudo
     CDS             complement(8822..8914)
                     /locus_tag="VM406_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003293.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            complement(9070..9579)
                     /locus_tag="VM406_13660"
     CDS             complement(9070..9579)
                     /locus_tag="VM406_13660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HVL77055.1"
                     /translation="MPDLWIRPEQPADAADIEAVIAAAFQNAPHTSRTEQFIVNALRK
                     AGQLTVSLVAEDDSGIVGHVAVSPVTISDGSTGWYGLGPVAVLPERQDQGIGKQLMDA
                     ALGALRTAGAHGCVVLGDPAYYRRFGFKAEPNLVLPDVPVEYFQAIAFGNAVPRGTVT
                     YDQAFSALA"
     gene            complement(9579..>11424)
                     /locus_tag="VM406_13665"
     CDS             complement(9579..>11424)
                     /locus_tag="VM406_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HVL77056.1"
                     /translation="LKPLESLLEQKTRLQKLAGEAGADIADVAAELEQKEGEYMRLRQ
                     QRDELAARISADRAAIAALESKSALPEPENVRAMRRALLDAKIDHVMLPDIVEVTDTK
                     WQGAVEGMLRSYTSVVLLENAKDAAAAYRIAEKERYGHFIVSDRVRAPEVKDQSLLAA
                     VRFTDKAPGWLIEQLARVTRVESVEAGAKLGNTEEWITPEAYHRERRGGRSLFVEASR
                     YRFGDAGRVQRIAALQASLPRLETQEDQLTLKISKLAGEVSSLKSRIAGVDAAKELVA
                     RQPEFEEATLAAAPLKAARTEVGTRLGELFPLTKKATEDRTIADSNWQNAKRALADAE
                     SHMRVAHKRYEAERAEHAVRLAEIRKTWRHLPLAWRRLARRQELVAEYQNAHQVELRL
                     KHLAVDLARDDWETDSTVVDQHTRLTALLQGRQAETEERRYQNNRAIEATTNARGAYI
                     ERLRYTIKTYSKNIKELGELAGIEVHTDPVRLENDDVQLAQAGLHVRFKFDGKGPIGL
                     NDGEASGGQQVMKSLILLIGLLKSDDGSGGFVFIDEPFAHLDIRNIQLVGEFLKNTDA
                     QYLMTTPLTHNTDVYDPSELTLITSKKKKEEKWAQPIFVLQRRVEKVA"
BASE COUNT         2064 a         3548 c         3506 g         2306 t
ORIGIN      
        1 gtggtgtacc cagacgcaca acacagcttt gacaacgcgc ggaccctcga cggaacgtgg
       61 acgaaggccg atgttgacgc caaggcgaat gcggaaacat tcgtgatgaa gagtctgcag
      121 gattatttca agtagcagga attaagggga tcatgcggtt gtcgacccct atcttttctc
      181 ctgcaacgaa taaatagacg aatacccgtt gctgaaatcc ggcggcagct cgatcacacc
      241 cgcctcgacg cgcgcaaacc gcgccggttc tccacgcacc atccggttgc gatggcaact
      301 gtcgatcacc ggcgccgtat aggcgaaggc atcggcggcg gcgtactgga tgatgtaagt
      361 ggtgcggatc tggtcggaca agttggcgcc cgagccgtgc accaccaggc catggtgcgc
      421 aacgatgtcg ccggggtcga gctcgctatc ctcggacagc gcaaagtcgg cttcacgcat
      481 ggtgtgattg cagttcagca tgtacttgcc gtcggcccag tggctgcgcg ggccctgctt
      541 gtggctgccg ggtatcgtct gcaggcagcc gttgccggag cgggaagggt tgagcggcac
      601 gctcaacgcc accaggttgg tgttcgtatg cgggaacacc ggccagtcct ggtgccagcc
      661 gatttcggcg ccgccgttcg gtagcttgaa gttgaccttg gagtggtaga acttcaccgg
      721 cccgcccacc aagtcggcgg cgatgtctcc cagcaccgaa tggaaggcga cctcgatgaa
      781 tgccgggtcg agttcggtcg ggctggagat gcgccggatg cgcgggttgt ccggccggtg
      841 cgcctcttcc aggtcgaaat gccgggtctt tgcttgcagg gcgctgcctt ccttgtacag
      901 ccgctcggtc gcttcggtca ggcgctgcag caggtcctgg ccgaagatgc ctcgcagcgt
      961 gagaaagccg gtttcgcggt attcgttcaa ctgcgcctcg ttgagcacgg ggcgatgcgg
     1021 gggcacttca atcggcattg ccttcaggtc ggtgatcgca ttcatgccgt cacctccgag
     1081 actgctgcct gccggcccag gatgtcgtcc ggtcctgcag ccagtggcga gaagttcgtc
     1141 gaacgcacgt agtccggacg cggattgggc acctcgtgcg ggcggttcac gaccgggttg
     1201 tagacgatgt agatagccca gcggtcgtcc tgcgaaaggt tgtggccgga tgcgtgcagg
     1261 atgttcggat ggaagaacac caccgtgccc ggcttgccgg tgatcggcac cggttccggg
     1321 ctgcgctcca tgatggagag catctgctgc ttcggcacca cccagaattt atagccggtc
     1381 gcttcatcga actgcggctc aaggttgcct tccttgtggc tgccggggat gaagtagagg
     1441 caaccgttga gctgcgtcgg ctcgtccagc atgatgagcg cggtgctcag gttcggctcc
     1501 ggcacgccat ccttgcgcca ggtgccgtag tcctggtgcc actgccacac tgagccgtcg
     1561 atcgcggtct tcaggttgca cttggtgtga tagatgtata gcgcatcgtc gccgagcacg
     1621 tcctgcgccg gctccagcat gcgcgggcag cgcgaagctg cgtggaagac cggcgaggcc
     1681 gtcggtccgt tgtcttcatg tacgcggtag atcgtcttcg cgacaccgcc gctgcgctcg
     1741 cgcacgagat ggtcggtatc gatctgctgg atgcgggcaa gctcggcctt catcgcatcc
     1801 acttcctgca tcgaaaacag ccctggcatg accaggaagc cgtcgcgttc gtaagtctcg
     1861 agctggtctt tcgtcagccg catggcgctc tcctttgttg tggacgggag aggaactccc
     1921 gtgtctcctc gagcggtccc cgcgccgggc gcgactcccg ccattccgtt tcatcttagg
     1981 catcggacaa ctatccgtaa aatatataat tggttcgata ttcataccaa tcgggtatca
     2041 aatggacatg cgtcacctgc gctacttcat tgcggtagcg gaagagctgc acttcacccg
     2101 cgctgcgagc cggctgcaca tcgcgcagcc gccactcagc cggcagatca gggagctgga
     2161 agaagaactg ggcgtgaccc ttctgcatcg aaccaagcga cgcatcgagc tgaccgacgc
     2221 cggccaggtc ttcctggaca aggcgcgcca ggtgcttgcc gccgccgatt ccgccatcgt
     2281 ggaaacgcag cgggcacaac gcggacagac cggaaagatc gccgtcggct tcttcgagca
     2341 gaccgcctac acgctgctgc cgccgattct gcatgccttc cagaagcgct ttccggaagt
     2401 cgatgtgcag ttgcgctggt atccggtggt cgagcaggtc aaggccttgc agcgtggcga
     2461 caccgatatc gccttcgtgc gcccggccgt cgacttggga gaggtgtcca ccgaactact
     2521 gctgaaggaa cccttcgtac tcgccgtgcc gcgcgatcac cgcttcgcgc agcaggaagt
     2581 cgtgtcgtta aaggagtgcg cgggcgagcg gctcatcaac tacacccaga cgctggcgcc
     2641 cgactactgc gccgccatca cccaggcatg tgccgcggct ggcatcgtgc cgggagactt
     2701 tctcgaagtg gggcaggttt acaccgcgct cggccttgtc agcgccggcg tcggtctcgc
     2761 cttcgttccc gcctcggtgc agcgggtgcg cgtcgacaac gtggtctaca ggccgctgcg
     2821 ggaaaaacgc ctgcaaagtc aggcgtatct cgcctggacg cggccgcatc cttcggcgct
     2881 gctgacatcc tttgtcgctg tcgcgaagcg cgttgtcgcg gagaaatttg aatgatccgg
     2941 ggcatgatgc tcctgtaaaa aaaatccttc cagggctgtc caccatccag atggatggga
     3001 accggatggt gctcggcgtg tctcacaagg caggaggcac gaaatgaacg caccccagga
     3061 agcccggctc aaggttgccg agactgaaaa aatcacgatc aacctcgggc ctgtcgacct
     3121 tggccacatt gatctgctcg tgcaggaagg gttttattcc aatcgcacgg atctgatacg
     3181 aactgcaatt cggaaccagt tgcaggtgca cgcggatgtc atccgccaga ctgtggcgcg
     3241 caagcggctg gtgctggggt tgcagcactt cacgcggagc gaactcgaag ccgcgcgcga
     3301 tgccggcaca aagctgcaga tccaggtatt aggtcttgcc agcatcgcga ccgatgtcac
     3361 tcccgagctg gccgttgccg ccatcgactc catcaccgtg ctgggcgtct tgcaagccag
     3421 ccccaccgtc aaggccgccc tggccggacg aattcactaa ggaatttcat gaaactcaac
     3481 gaacgctttc tcgcgcaaat gcgcgaagcc accattttgc tcgagagcga aggcccgatg
     3541 gccgcgaccg ccgccatcca gcgcgccttg aacgcgcact ccgggcctgc tggcagaccg
     3601 aatcagcctg cgcaaggcgg gaggggcgca gagccggccg aggatgccac gccgctgccg
     3661 agcacatccg atgccggcgc tgcacgcacg gacgcctggt tcgacagcat ccgccgtcag
     3721 gcagccgatg ccgtgcagga tgtcgaattc agagaaatgg aaagtacaga atcgggcagt
     3781 gtcggttctg acagtgcaga cagtgcaggc agtggccgtt ttctgtctgg cgcatgcagc
     3841 aattcagccg gcacgcgcga atacaagctg tacgtcccgc gcggttatgt cggccagccg
     3901 ctgccgctga tcgtcatgct tcacggctgc acccaggacc ctgacgattt cgccgccggg
     3961 accggcatga acatactcgc cgacgaacag aacttccttg tcgtctaccc ggcccagagc
     4021 aaaaacgcca atacatccaa ctgctggaac tggttccagc ccacacatca ggcgcgcgac
     4081 cgcggcgagc cctcgctgat tgcggacatc acgcgcaagg tcgtgcgcga gtaccaggtt
     4141 gacgaacggc gcatctacgt tgccggtctt tcagcgggcg gtgccatggc agccgtactc
     4201 ggcgcgcgtt atccggacct gtatgccgct attggcgtcc attccggctt gccgctcggc
     4261 gtcgcgcgcg atgtcgtctc cgcattcgca gcaatgaaag gcggcaaggg aaaagcatcg
     4321 tcgcgcgctg ccaccgcaca tcctcccgcc acgctcggac acaccatccc cgtcatcgtc
     4381 tttcatggcg atcgcgatcg cactgtgcac gggtccaacg gcgacaaggt tctggagcaa
     4441 tgcatagcag cgggagaccc ggcgtgcgca aaagcgaacg tggaggtcgg catggcaaga
     4501 gggcggcgct acacgcgcgc cgttcatcgc gacgcgcagg gcagggcgta tgcagaaaaa
     4561 tggaccgtgc acggtgcggg ccacgcatgg gcaggcggga gcaaaaaggg gtcttacacc
     4621 gatcccggcg cgccggacgc ctcccgcgaa atggttcgct tcttcctgag ccacgcactg
     4681 ccaggctccg actgacgcga gacgcccctg tacaaggccg tcgcacgatc cggaggaatg
     4741 cgaccttgcc ttcaagcgct tgtttgtcgc gcccctcggc ggcccgccga ggggcccggc
     4801 aagatcatcc gcttacatga acggcgctgg cccgtgcggg gttgttactt tttcggcttg
     4861 gttgcgcgct tcggcggcac ctgtggcttc gacttcttct gttgccggaa aatcgtcctc
     4921 gcatcctgca cggcgcgttc ctgcgtgcgc atttccggca gatcatcgtg cagcgtaaaa
     4981 aaatccgccg cctgcgcgcg catcaccatc ttgattgcct cttcatcggc caagtcgtac
     5041 agctcggtca gaagcgtggc aacttcgtcc agaaattctt cgtaggtcat ctcgtaatgg
     5101 ggcagatcct gttttcggac gcaaagcttg aataagctcg gggcgaataa actcgagttt
     5161 agtcggtcca atgccctcca ggcgctctac gccgcttcct cctgaggaac cacactctcc
     5221 caccacactc gggcggcttc ctccccccgt ttgtcgcgct cgtagtagta accagcctcg
     5281 gccgcgcgct ctgtcaggct gcggtcagca cgcgcagccg tcgccaggta ttccagtcca
     5341 cgatcgtcgt cttcaccgag caggatgcgg ccatagtagt acgaggcctt ggggaatggt
     5401 ccgccgggct tcttcagtaa atgctccagc gccggcttcg cggcgagcgg ggactcgaac
     5461 tcagccttta gcaaggcaag ttcctgcaga tcgtgcaggg ccagtgcgtc cagctgatgc
     5521 tgcgccaatt cgctcagtcg cttctgcgag cgctgcacgt agcgatagcg cgcctcccat
     5581 tccttctgct ccgctttcca ccatgtctgg tcgaattcca cgatcaagcg tcgagccgtg
     5641 gcggcgccga gcaatgcttc tgccgccgtc acttccagcg gcgccggcaa ttccgcttca
     5701 tggccggtgg cttcgacccg atcgcgcaga gacgggtggg tgtcgtgcag tccggattgt
     5761 tcgcgccagg cttgcatgag tcgttcacgt gtggcccatt gctcgtagct cgccttgaac
     5821 gcggtgcgca tggacgcgaa gggcatgatg gcagggcggg ggattgtatt cgcctgcttg
     5881 aagatggtcg accagaactg ctcgtggatc cagcgcccga gcagcgcatc gcgaatcagc
     5941 ccggacgcgt tggcctcggc gccgaccagg tctgtcgcgg tcaagtccgc ctcatattcg
     6001 ttctggcgcg agagcacgaa ggtataggcg ttgtagtagg gcatgaagcg gtctagcgcc
     6061 atggcgagca aaccgtggac ccagttgtct tcacggctgg cgtcgacctg ctcgtacagg
     6121 gcgccaaagg tgcgtcgttg gcggtagacc caggaggaca gcttgccgtg gttgccgcac
     6181 aggtgcccgt attcgtgcgc aacagtggcc agcatttcct ttggcggcac accgagcaaa
     6241 taaggcagac ccaggatcag atagctggtg tgcccgccaa acatgcccca gcgcggaatc
     6301 tgcgcaatgg cggcgttgta tgcgtcgtca atcagcacat gatggattgg cggccccttc
     6361 agcttgcggc gcatcttgtc gagcacttcg aagagcttcg gcgcttcttc gcggccgatg
     6421 cgtctgccct gtggtggagg cagccgcatg aagaacatgc gcagcaccgc aaagaacacc
     6481 ggcaccatcg tcacggcgaa caaggcaatg gtgatcaggc tggcgcgctt tccggcatac
     6541 gcccactgaa ttccggcgta aatggccaca gcgatggccg ccagcgcgac aaacaaagcg
     6601 acgtacgctg ccgcactgat caacacgacc ttgctgcgaa aggcggccgg actgttgagt
     6661 gcctgcgctt ccagccgatc caccagtcct tcatagacgt agcgatccaa accttgttcc
     6721 tccctgtcac agcgggcaga tcatgcattt acgtttccaa gccgcctgca ccattccatg
     6781 cgccctcgca gttacacata cacaagcttc cgcgcgtgca tcatatcccc agcggccgat
     6841 gatccgccag cgccttccac gaatcgaact gcggggcctc tgacctgagc agttatatcg
     6901 gatcaagcaa gctcggggta gctccagttg ataaaccccc atttcctcat gccggtcgct
     6961 tctggcgcgt cgtaactcgg acttgaatct ggcctcactc gatcgaagtg ctactgccag
     7021 ttctcaatca ccagcctgct cctgaaccat gcggtggcaa gggattcgtc aagaaactgc
     7081 tggcgagctt tccatcgttc cgttctgcca cggttttgcc ggggtcgatc aggggtgcac
     7141 tcgattcctt gccccattat tcctttcggt agcgctgtgc cgacgaactc tgtcgccgca
     7201 caggaagcga tttgcatagt ggacgttgat cagctgcgag agatcattga tgactgttgt
     7261 ttgcatcagc acacgagttc gtcgatatcg ctgcccgggc gcaatcgcat gcagtgcgcg
     7321 agtttgtgtt ttgtttgccg agggaagggc gcgaaagcgc catcttggtt cagatcaaac
     7381 cattgaatcc aatggtccac actatcttca gccgccgcat ctaatccccg ctggctgtat
     7441 caagtcgcac tcgccggcag gcaccagatt tcatcaaggt attggttgca acatcaaaaa
     7501 atgaggttcg ccatgaatac tgcaagaacc gttcaaaccg tcctggctgc tgcggttgta
     7561 ggtctgtgcg gcagctccgt ccacgcgcag gaattctcga cgcaattatc cggattcgac
     7621 gaggttcccc tggcaatctt tactgcgggt agtggcacgc ttgatctcac gctggatcaa
     7681 caagcaggga ccgtcaatta cacgctctcc tattcggacc tgtccacgcc ggtgacgcag
     7741 gcccatattc acttcggtaa ggagcgtgtg gcgggcggcg tgatcgcttt tctctgttcc
     7801 aatctgacgg aatccccacc gggaacacct gcatgcccag agattagcgg aacggtaagc
     7861 ggctcgatca ctgccgatgg cgttgtcgga atagagccgc aaaatgtcga agcgggagat
     7921 ttcagtgcac ttgttgctgc gctcacatcc gggaccagct acgtcaatgt gcacaccaag
     7981 aaatttccag ccggtgagct gcgcggcgag attcgcggtg aggtgccaga tcagagtcct
     8041 ggtgacggcc ccggccagag ccctggtcag agccctggtc agagccctgg tcagagcccc
     8101 ggacagagcc ccggacagac gcccagccct gcaacgccag ggacgcccta caactagcgt
     8161 tcaagctgac tttgacaggc caagggcgga gtagctgccg atacttgtgt ctgccagatg
     8221 acttgtaggc atttagacga atctgccgtg ttgcgccgag aagcaggagc gctcctttgc
     8281 agcgaaaagg ttgggaaagg gagttgtgag gtttgagcac acgcctttgt gtacataatc
     8341 atcacacgcc cttcctggcc agtccgcacc gcaacctgcg cggtgctcgg attcgagcag
     8401 cacgaagctt tgttttgcga aaccacgctc tcatccgctg gggcgggcgc tgtaagctga
     8461 atcagtgcga ttcccgagtt cccggcttga agcgctcgcg tggcagctgg aagcgtcagt
     8521 cttcgtccgt tgaagcagaa atgcgcttga gccgctgacc gtagacaccc cacacggtct
     8581 cgacgccatc gatggtgccg accaatgccc agcccgtgcc gatcttgcgc gcttcgacgg
     8641 gaatacccaa ttcgttgccg acctgctgag cccactgctt cgccgcgcct tgccctttga
     8701 agagctcctg gccgtcgaat atcaccgtgc cgtcgaacac gggcttggtg aatacactca
     8761 tccaacactc cttcaaaagc tgtatcgaac gtcctggccc gccattgtca gacaaatcgt
     8821 tcagttcgta actggtactt ctgccgcccg catcagactt cttcagcacc ccgcgtgcca
     8881 gcagatcatt gaggtcgcgt agtgccaatt cggccagtcg gcgtcctgcc aggtataagc
     8941 gtcatctccg ctattcatgc ggagattatg tttcgcgacc gccgcatgtg acgtcttgtt
     9001 caccgtacga aacgccgcgc cgccaaggat catgctcttg cggcatgcgt ggccgatgcc
     9061 agccatattt caggccaatg cactaaacgc ctggtcatac gtgaccgtgc ctcggggcac
     9121 agcattgcca aaagcaattg cctggaaata ttcgacgggc acatccggta aaaccaggtt
     9181 gggctcggcc ttgaaaccga atcgccggta gtaggccggg tcgccaagaa ccacacaacc
     9241 gtgcgcgccg gcggtgcgca gcgcacccag agcggcgtcc atcagttgct tgccgattcc
     9301 ttgatcctgg cgctccggca ggaccgcgac gggaccgagc ccgtaccacc cggtactgcc
     9361 gtccgaaatc gtcaccggcg aaacggcgac atgaccgacg atgccgctgt cgtcctccgc
     9421 aaccagcgag accgtgagct gtccggcctt ccgcaatgcg ttgacgatga actgttcagt
     9481 gcggctggta tgcggggcgt tctggaaagc ggcggcgatg acggcctcaa tgtccgccgc
     9541 gtccgcgggc tgctcgggtc tgatccataa gtccggcatc acgccacctt ctcaacccgc
     9601 ctctgcaaca caaagatcgg ctgcgcccac ttctcctcct tcttcttctt gctcgtgatg
     9661 agcgtcagct ccgaaggatc gtagacgtcg gtgttgtgcg tgagcggcgt cgtcatcagg
     9721 tattgcgcgt cggtgttctt caggaattcg ccaaccagct ggatgttgcg gatgtccagg
     9781 tgggcgaagg gttcgtcgat gaagacgaag ccgcctgagc cgtcgtcgct cttcaacagg
     9841 ccgatcaaca ggatcagcga cttcatcacc tgctggccgc cggatgcctc tccgtcgttc
     9901 aggccgatcg ggcccttgcc gtcgaacttg aagcgtacgt gcaggccggc ctgggctagc
     9961 tggacgtcgt cgttttccag gcgcaccggg tcggtgtgga cttcgatgcc ggccagttcg
    10021 cccagctcct tgatgttctt gctgtaggtc ttgatcgtgt agcgcagccg ttcgatgtag
    10081 gcgccgcgcg cgttagtggt agcttcgatc gcgcggttgt tctggtagcg gcgttcctcg
    10141 gtttccgcct ggcggccttg cagcagggcg gtgaggcggg tgtgctggtc aaccaccgtg
    10201 gaatccgttt cccagtcgtc gcgggcaaga tcgacggcca ggtgcttcag gcggagttcg
    10261 acctggtggg cgttctggta ttcagccacc agttcctgcc ggcgcgcgag gcggcgccag
    10321 gcgagcggca ggtgacgcca ggtcttgcgg atctcggcca gacgtaccgc atgttcggcg
    10381 cgctcggctt cgtagcgctt gtgggccacg cgcatatgcg attcggcgtc ggccagcgca
    10441 cgcttggcgt tctgccagtt gctgtcggca attgtgcggt cttcggtggc tttcttcgtg
    10501 agcggaaaca gttcgccgag gcgggtgccg acttccgtgc gcgcggcttt gagcggcgcg
    10561 gcggcgagcg tggcttcttc gaactccggc tggcgcgcga ccaattcctt tgctgcatcc
    10621 acacccgcga tgcgcgattt cagactgctg acttcaccgg cgagcttgct gatcttgagc
    10681 gtgagctggt cttcctgcgt ttccaggcgc ggcagcgagg cttgcagcgc ggcgatgcgt
    10741 tgcacacggc cggcatcgcc gaagcggtag cgcgaggctt cgacgaacag cgagcgtccg
    10801 ccgcggcgtt cgcggtggta ggcctcaggg gtgatccatt cttcggtgtt gccgagcttc
    10861 gcgccggcct cgaccgattc cacccgcgtc acgcgcgcga gttgttcgat cagccagccg
    10921 ggcgccttgt cggtgaagcg cacggcggcg agcaggctct ggtccttcac ttccggcgcg
    10981 cgcacgcggt cggaaacgat gaagtggccg tagcgttcct tttcggcgat gcgataagcg
    11041 gcggctgcat ccttcgcgtt ttcgagcagg acgaccgagg tgtagctgcg cagcatgcct
    11101 tcgaccgccc cttgccactt ggtgtcggtc acttcgacga tatcgggcag catgacatgg
    11161 tcgatcttcg cgtccagcag cgcgcggcgc atcgcgcgca cgttttccgg ctcgggcagg
    11221 gcggatttgc tttccagcgc ggcgatggcg gcgcggtcgg cgctgatgcg ggcggcgagt
    11281 tcgtcgcgct gctggcgcag acgcatgtat tcgccttcct tctgctccag ctcggcggcc
    11341 acgtcggcga tgtcggcgcc ggcctcgccg gccagcttct gcaggcgggt cttctgttcc
    11401 agcaggcttt ccagcggctt gagc
//
LOCUS       DATLGF010000447         3587 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_11902,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000447 DATLGF010000000
VERSION     DATLGF010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11902"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VM406_13670"
     CDS             complement(<1..380)
                     /locus_tag="VM406_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVL77057.1"
                     /translation="MTDSPIAVHAGPKALAHMREHGMRAADVAVIPAAAGGPKGLIFE
                     RFDQWLFGQWLPSAPRERILIGSSIGAWRMAAACQADPAAAFRRLADLYCGQRYPHKP
                     SPQYVSQVSRDFVAGLMSGHEQAVL"
     gene            complement(377..949)
                     /gene="pth"
                     /locus_tag="VM406_13675"
     CDS             complement(377..949)
                     /gene="pth"
                     /locus_tag="VM406_13675"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HVL77058.1"
                     /translation="MSIRLIAGLGNPGPEYEQTRHNAGFWLVDNLSPIPLSREARFNA
                     LAAKIRIAGEDVWLLEPQTYMNRSGQSVGALARFFKIPPEQILVVYDELDLPPGAAKL
                     KKGGSSTHNGLKDITAALGTRDYWRLRLGIGHPRDMNSQQQVVDYVLHRPRKEEQEQI
                     ENAIRNSLDVIPLLVEGKFEAAMMKLHSAK"
     gene            complement(1030..2742)
                     /locus_tag="VM406_13680"
     CDS             complement(1030..2742)
                     /locus_tag="VM406_13680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL77059.1"
                     /translation="MFEPISIPAEHFQRIFESAPGSFLLLLPTPAFTIAAVTDDYLRD
                     TLTTRAHLLGKPLFDAFPDNPSLPEAHATRNLRASLERVLATRSSDMMAIQRYDVPRR
                     DGTGFEVRYWTPINTPVLSAGGDILYIVHRVKNVTDYVRLQEENEAQHKQSAELDARN
                     RRMQAEVLQQSMELDAKNRELRQVNEALREYAQKARDEARHKDEFLAMLAHELRNPLA
                     GISSALELLQLGEGDQTKNAQLHEVCRRQLGNLVRLVDDLLDVSRISRGAVALRMERL
                     DLRDVVESALYAVRSVFDDKGLAVATTIRPGAYQVLGDATRLEQVLSNLMVNAAKFTQ
                     AGGKVEVTLANEQQGQWTWSVVQVTDSGRGIPQEKLDAIFDLFVQVDAGIDRARGGLG
                     IGLPLARKLAEMHGGSIVAESRGTGFGSSFIVRLPNDADSGPVAPGDDAELPKFSARS
                     GTRVLVIDDNADARETLKSLLEAYGFCVDTAATGEEGLERLLARCHEIALVDIGLPGL
                     DGFEVARKVRRAPSASHTRLVALTGYSGPETERRVVEAGFDLHLVKPVRATELQRIID
                     VPTR"
     gene            complement(2960..3556)
                     /locus_tag="VM406_13685"
     CDS             complement(2960..3556)
                     /locus_tag="VM406_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HVL77060.1"
                     /translation="MKVVAFPRSEQGSGASRRLRRSGQTPGIIYGGEAAPVTIALDHN
                     ALFHALKKEAFHSSILDMEIEGKLQKVLLRDFQMHAFKPIVLHVDFQRVDEKQTIHTK
                     VPLHFINAEISPAVKLGGGIINHVLNELEVSCLPADLPEFIEVDLSKVEAGATVHLND
                     ITLPKGVTPVLHGQDNPTIATAVASAGAQSAASEGEAQ"
BASE COUNT          605 a         1099 c         1187 g          696 t
ORIGIN      
        1 agtaccgcct gttcgtggcc ggacatcagg ccggctacga agtcgcgcga aacctgcgag
       61 acgtattgcg gcgagggctt gtgcggatag cgctgcccgc aatacagatc ggccaagcgc
      121 cgaaaggcgg cggccggatc ggcctggcag gcggccgcca tgcgccaggc gccgatcgat
      181 gagccgatca ggatgcgctc gcgcggagcg gacggcagcc attgcccgaa cagccactgg
      241 tcgaaccgtt cgaagatcaa ccccttcggc ccgccggccg ccgccggaat gacggcgaca
      301 tccgctgccc gcatcccatg ctcgcgcatg tgcgccaggg cctttgggcc ggcatggacg
      361 gcgatcggac tgtcggtcat ttcgccgagt gcagcttcat catcgcggct tcgaacttgc
      421 cttcgaccag cagcggaatc acatccaggc tgttgcggat cgcattctcg atctgttcct
      481 gctcttcctt gcgtggacga tgcagcacat agtcgaccac ctgctgctgc gaattcatgt
      541 cgcgcggatg gcctatgccc aggcgcaggc gccagtagtc gcgcgtgccg agcgcagcgg
      601 tgatgtcctt caggccgttg tgggtggacg agccgccttt cttcagtttc gcggcgccgg
      661 gcggcaggtc gagttcgtcg tagacgacca ggatctgctc gggcgggatc ttgaagaagc
      721 gcgcgagcgc gccgaccgac tgcccggagc ggttcatgta ggtctgcggc tccagcagcc
      781 agacgtcctc gccggcgatg cgtatcttcg cagcgagcgc attgaagcgc gcctcgcgcg
      841 aaagcgggat cggtgacagg ttgtcgacca gccagaagcc ggcgttgtgg cgggtttgtt
      901 cgtattcggg cccggggttg ccgaggccgg cgatgagtcg gatggacatg ggtgcagcgc
      961 ggtgcggcgt tgaaaacaag cgattatccg gcaagagccg attctttacc tgtcgcaagc
     1021 aggcgggctt cagcgcgtgg gcacgtcgat gatgcgctgc agttccgtcg ctcgaaccgg
     1081 cttgaccaga tgcaggtcga atcccgcctc gaccaccctg cgttcggtct cggggccgct
     1141 gtagccggtc agggccacca gcctggtatg gctcgccgag ggagcccggc gtaccttgcg
     1201 tgccacctcg aagccatcca ggccgggcag gccgatgtcg acgagggcta tttcgtgaca
     1261 gcgcgccagc aagcgttcca agccctcttc cccggttgcc gccgtgtcca cgcaaaagcc
     1321 gtaagcctcg agcaggcttt tcaaggtttc gcgcgcgtcg gcattgtcgt cgatgacaag
     1381 gacgcgggtg ccggaccgcg ccgaaaattt gggtagctcg gcatcgtccc ctggagcaac
     1441 agggccggaa tcggcgtcat tgggcagtcg aacaatgaag gaactgccga atccggttcc
     1501 ccggctctct gcgacgatgc tgccgccgtg catttctgcg agcttgcgcg ccagcggcag
     1561 gccaatgccg agtccgccgc gggcgcggtc gatgccggcg tccacctgga cgaacaggtc
     1621 gaagatggcg tccagctttt cctgcggtat cccgcgtccg ctgtcggtca cttgcacgac
     1681 cgaccatgtc cattgtccct gctgctcgtt ggcgagcgtg acttcgacct tgccgccggc
     1741 ttgcgtgaat ttggcggcgt tgaccatcag gttgctcagc acctgctcca ggcgcgtagc
     1801 atcgcccaac acctggtatg cccccggcct gatcgtggtt gcgacagcca gtcccttgtc
     1861 gtcaaagacg ctgcgtactg cgtacagggc actctccacc acatcgcgca ggtccagtcg
     1921 ctccatgcgt agcgcgacgg caccgcggct gatgcgggat acgtcgagga gatcgtccac
     1981 cagccggacc agattaccga gctgacgccg gcagacctcg tgtagctgtg cgttcttggt
     2041 ctgatcgccc tctccgagct ggagaagttc cagcgccgag gagatgccgg cgagcgggtt
     2101 acgcagctcg tgcgcgagca tggcgaggaa ttcatcctta tgccgcgcct cgtcccttgc
     2161 cttctgcgcg tactctctga gtgcctcatt gacctggcgc aactccctgt tcttcgcatc
     2221 cagttccatg ctctgctgca gcacctcggc ctgcatcctg cggttgcgtg cgtccagttc
     2281 ggcgctttgc ttgtgctgcg cttcgttctc ttcctgcagg cgcacgtaat cagtcacgtt
     2341 cttgacgcgg tggacgatgt agaggatgtc gccgcccgcg gacagtaccg gcgtgttgat
     2401 cggggtccag tagcgcacct cgaagccagt gccgtcgcgc cgcggcacgt cgtagcgctg
     2461 gatggccatc atgtccgaag accgggtggc cagcacccgt tcgagcgacg cacgcaaatt
     2521 gcgcgtcgcg tgcgcttcgg gaagggaggg attatccgga aaggcgtcga agagcggctt
     2581 gccaagcagg tgtgcgcgcg tggtcagcgt gtcgcgaagg taatcgtcag ttaccgcggc
     2641 gatcgtaaat gccggagtcg gcaggaggag gagaaaggat ccgggggcac tttcaaaaat
     2701 gcgctgaaag tgctcggcag gaatcgagat aggttcaaac attggcacgg acaagggagc
     2761 ggatgttgtt ttgacctcga tgccggcaag tgttcttcaa atctaggcgg gatcgtgcag
     2821 accgcctttc gtccgatgtg cggtccgggc tttgccgggc gtgcggtcca ctgttcgcat
     2881 caggaagcag gtgtgaggcg tcggcgaaaa agcaaaaccc cgcctcaggg gcggggtttt
     2941 gcggcgaaga gcgaaacgct tactgcgctt cgccttcaga tgcagccgac tgggcaccgg
     3001 ccgaagcgac ggcggtcgcg atcgtcgggt tgtcctggcc gtgcagcacc ggggtcacgc
     3061 ccttgggcag ggtgatgtcg ttcaggtgga cggtggcacc cgcttcgacc ttggacagat
     3121 cgacttcgat gaactccggc aggtcggccg gcaggcagga cacttcgagt tcgttcagca
     3181 cgtggttgat gatgccgccg cccagcttca cggccgggga gatttcggcg ttgatgaagt
     3241 gcaggggcac cttggtgtgg atcgtctgct tctcgtccac gcgctggaag tccacgtgca
     3301 gcacgatcgg cttgaacgcg tgcatctgga agtcgcgcag caagaccttc tgcaacttgc
     3361 cttcgatttc catgtcgagg atcgacgaat ggaaggcttc cttcttcagc gcgtggaaca
     3421 gcgcgttgtg gtccagggcg atggtcaccg gggcggcttc gccaccatag atgatgccgg
     3481 gggtctggcc cgagcgacgc aggcggcggc tcgctccgga cccctgctcg ctacgcggga
     3541 atgcaacaac tttcattttc agctccaaat aaagcaagga cttgcct
//
LOCUS       DATLGF010000448         7435 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_201076, whole genome shotgun sequence.
ACCESSION   DATLGF010000448 DATLGF010000000
VERSION     DATLGF010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 7435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7435
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201076"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VM406_13690"
     CDS             <1..747
                     /locus_tag="VM406_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HVL77061.1"
                     /translation="GLKPVVAIYSTFLQRAYDQLIHDVALQNLDVTFALDRAGLVGAD
                     GATHAGNYDIAFLRCIPNMVVMAASDENECRQMLSTAYQYPGPAAVRYPRGAGIGAQI
                     EKTLATLPLGKGEIRRLGERIAILAFGTMVAPSLAAGETLNATVANMRFVKPLDRELI
                     AQLAASHDALVTVEEGCVMGGAGAAVAEALADAGIVKPILHLGLPDKFIDHGDAGQLL
                     AACGLDAKGIVASIQKRFDANEPRLVVNNN"
     gene            complement(784..1341)
                     /locus_tag="VM406_13695"
     CDS             complement(784..1341)
                     /locus_tag="VM406_13695"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HVL77062.1"
                     /translation="MGNRLSKIATRTGDNGTTGLGDGSRVGKDSLRVHAMGDVDELNS
                     HIGLLLCEDMPEAMREELISIQHDLFDLGGELCIPGYTLITETHVARLDGLLQKYNAD
                     LPPLKDFILPAGSRAAALAHVCRTICRRAERAIVTLGNAETINANPRHYMNRLSDLMF
                     VLSRVLNRFAGGSDVLWEKGRKRDA"
     gene            1628..3040
                     /locus_tag="VM406_13700"
     CDS             1628..3040
                     /locus_tag="VM406_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HVL77063.1"
                     /translation="MNQPAPLSTLKKPFPDELLAALQAIFEDRVTTSQAMRDHHGRDE
                     SSYDPMPPDAVVFAHTTEEVAQAVKLCAAHRVPVIPYGTGTSLEGHVLALRGGVTIDL
                     SQMNQVLAVHAEDQTVTVQAGVTRKQLNQEIRDTGLFFPIDPGADASIGGMAATRASG
                     TNAVRYGTMRENTLALTVVTADGRIIKTGTRARKSSAGYDLTRIFVGSEGTLGIITEV
                     SVRVYPQPEAVSAAVCSFPSTADAVNAVIQTIQMGVPVARVEFLDENSVRAINAHDKL
                     TLPEKPLLLFEFHGSEHGVQEQAQIVQDIAGEHNAIGFEWATRPEDRSRLWTARHNAY
                     FALLQMRPGGRAITTDCCVPISRLAECILDTQADCEHNGMIYGIVGHVGDGNFHVLMM
                     IDPSDPADIAKAESVNARIVARAIAMDGTCTGEHGVGLHKMDFLVQEHGEEAIETMRM
                     LKRAFDPENILNPGKIVHLD"
     gene            3081..3980
                     /locus_tag="VM406_13705"
     CDS             3081..3980
                     /locus_tag="VM406_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVL77064.1"
                     /translation="MAIRLPPVHALSAFEAAARLGSFALAADELCITPSALSHRIRLL
                     EDLVGERLFARDGRAVSLTEFGRRYLDVVRGALRALTEFPLPHKSAPVQPRVKITVPP
                     TFARCLLVPRLAEFTARHPEIVIELFLSVPLYDLSLSESDVEVRFGAGNYPDLVTEKL
                     FEEPTFAVASPAYLAKVGPIDTPADLKKATLLRSALEPWQPWFEVAGLDWPEPTTGLR
                     LDDLGLLLEAVRHGHGVGLTRKHFAQKHIESGELVHLFPDIQLSAPPHAYYVVYGRQV
                     RERPEVDAFIAWLRGAFAPLTTS"
     gene            4118..5611
                     /locus_tag="VM406_13710"
     CDS             4118..5611
                     /locus_tag="VM406_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HVL77065.1"
                     /translation="MNAPVSPTTAFTPARQREVVDALRRVLPADCVLYDEEDTRPYEC
                     DGLSAYRQLPMVVALPRDEQQVIDALKTCRALNVPIVPRGAGTGLSGGAMPISDGVVL
                     STARLNRVLKLDPYARIAIVQPGVRNLAISDAANAHGLYYAPDPSSQIACTIGGNVAE
                     NSGGVHCLKYGLTIHNVLRVRMVTIEGDVVELGSEALDAPGLDLLAVFIGSEGMLGVV
                     TEVTVKLVPKPQQARVIMASFDNVVKGGDAVASVIAAGIIPAGLEMMDKTSSRMVEPF
                     VHAGYDTEAEAILLCESDGVPEEVDEEIARMSDVLRASGATAISVSQNEAERARFWSG
                     RKNAFPAAGRISPDYYCMDGTIPRKRLAEVLLAIAEMENKHGLRCANVFHAGDGNLHP
                     LILFDANQPGEFERAEAFGADILELCVKVGGTITGEHGVGIEKINSMCVQFSPQELEA
                     FFAVKRAFDTAFLLNPDKAIPTLNRCAEFGRMRVSGGQLKFPNLPRF"
     gene            5613..6665
                     /gene="glcE"
                     /locus_tag="VM406_13715"
     CDS             5613..6665
                     /gene="glcE"
                     /locus_tag="VM406_13715"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HVL77066.1"
                     /translation="MDSILQQFRDTVASASVGKQPLRIRGGGTKDWYGQSLAGEVLDT
                     RAYSGILAYEPTELVITARSGTPLVEIEAALAERKQMLAFEPPRFGPASTIGGVVAAG
                     LSGPRRQAVGAVRDFVLGATLMDCKGEVLRFGGQVMKNVAGYDVSRLLAGSLGTLGLI
                     LDVSLKVLPRPFAEITLRFEMRKTEAIEQLNRWGGQPLPISASAWHGGVLVVRLSGPE
                     PALRAAQQKMGGEVVEDAEPLWQGLRDQSNAYFGEGTLWRLSVPSTTPPLALKGEHLI
                     EWGGAQRWLRSDVDAATIRHAAATVGGHATLMRGDKGVGVFHPLPPAVMRIHRNLKTA
                     FDPSGIFNPGRMYPEF"
     gene            6668..>7435
                     /gene="glcF"
                     /locus_tag="VM406_13720"
     CDS             6668..>7435
                     /gene="glcF"
                     /locus_tag="VM406_13720"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HVL77067.1"
                     /translation="MQTNLADFIKHTAEGEEADAILRKCVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLMKQVLEGKEPTMATLTHLDRCLTCRNCETTCPSGVQYGRLVDIGRKVV
                     EARVERPLAQRAVRYALKEALPRKWLFGPAMKAGQIVRPLLPQVLKNKVPAPQQAGAW
                     PRAAHARKMLLLDGCVQPAMAPNINSATARVLDTLGVQLVVPRGTGCCGAIRYHMNDQ
                     AGGLDDMRRNVDAWWPHVEAGVEAIVMNASGCGVTVKE"
BASE COUNT         1264 a         2458 c         2491 g         1222 t
ORIGIN      
        1 ggtctcaagc cggtggtcgc gatctactcc accttcctgc agcgcgccta cgaccagctg
       61 atccacgacg tggcgctgca gaacctggat gtcactttcg cgctcgaccg cgccggtctg
      121 gtcggtgccg acggcgccac ccatgccggc aactacgaca tcgccttcct gcgctgcatt
      181 ccgaacatgg tggtgatggc ggcctccgat gaaaacgagt gccgccagat gctctcgacc
      241 gcctaccagt atcccggccc ggcggcggtg cgctatccgc gcggggcagg catcggcgcg
      301 cagatcgaga agacactggc gacgctgccg ctcggcaagg gcgagatccg ccgcctgggc
      361 gagcgcatcg cgatcctggc cttcggcacg atggtcgcgc ccagcctcgc tgccggcgag
      421 acgctcaatg ctacggtcgc gaacatgcgc ttcgtcaagc cgctcgaccg cgagctgatc
      481 gctcagctgg cggccagcca cgatgcgctg gtgacggtgg aggaaggctg cgtgatgggc
      541 ggcgccggcg ccgcggtggc ggaggcgctc gccgatgcgg gcatcgtgaa acccatcctg
      601 catctgggcc tgccggacaa gttcatcgat cacggcgatg cggggcagct gctggccgca
      661 tgcggactgg atgcgaaggg catcgtcgct tccatccaga aacgcttcga cgccaacgag
      721 ccgcggctgg tcgtcaacaa caactgacca ggacggatcg ccggcccagg gcagcgccgg
      781 gcttcaggcg tcgcgcttcc tgcccttctc ccacagcacg tcgctgccgc ccgcaaagcg
      841 gttcagcacg cgcgagagca cgaacatcag gtccgacagc cggttcatgt aatgacgcgg
      901 gttggcgttg atcgtctccg cattgccgag cgtgacgatc gcacgctctg cgcgccggca
      961 gatggtgcgg cacacgtggg cgagcgccgc cgcccgggat ccggccggca ggatgaaatc
     1021 cttcagcgga ggaaggtctg cgttgtactt ctgcagcagg ccgtcgagcc gcgccacatg
     1081 cgtctccgtg atcagcgtat agcccgggat gcacagttcc ccgcccaggt cgaacaggtc
     1141 gtgctggatc gagatcagct cctcgcgcat cgcctcgggc atgtcctcgc acagcagcag
     1201 gccgatgtgg gaattcagtt cgtccacgtc gcccatcgca tgcacgcgca ggctgtcctt
     1261 gcccacgcgc gagccgtcgc cgagtccggt ggtgccgttg tcgcccgtgc gcgtggcgat
     1321 tttcgagagt cggtttccca tggtggtcga agccgcggtt gaaagtgtcg agcatacggc
     1381 aaatccggcc gcgccgatgt catgcgcatg tcaatctttc cgcctgcccg gcctgacggg
     1441 ccaggagtag aatcgacgga cgacgcggac atgccgcgaa caataacgat aaaaagccct
     1501 aacaaaatgc cgatggcaag gcgcgccgac gcagacggta cgtgtagtac ggcaaggagg
     1561 cgcaacgccg ccagcggcat tttgttaggg cttccaagtc cgattccact gcccgaggag
     1621 ctaccagatg aaccagccag ccccgctttc cacgctgaag aagccatttc cggatgaatt
     1681 gctcgcggca ctccaggcca tattcgaaga tcgggtgacc acctcccagg caatgcgcga
     1741 ccaccacggc cgcgacgaat cctcctacga cccgatgccg cccgacgcgg tggtgtttgc
     1801 ccatacgaca gaagaggtcg cgcaggcggt gaagctgtgc gccgcgcatc gcgtgccggt
     1861 cattccctac ggcaccggca cctcgctcga aggccacgtg ctggcgctgc gcggcggcgt
     1921 gacgatagac ctgtcgcaga tgaaccaggt gctggccgtg cacgccgagg atcagacggt
     1981 gaccgtgcag gccggggtga cgcgcaagca gctgaaccag gagatccgcg ataccggcct
     2041 gttcttcccg atcgatccgg gcgccgacgc atcgatcggc ggcatggcgg ccacgcgcgc
     2101 ctccggcaca aacgcggtgc gctacggcac gatgcgggag aacacgctgg ccctgacggt
     2161 ggtgacggcg gacggacgca tcatcaagac cggcacgcgc gcgcgcaagt cgtccgccgg
     2221 ctacgacctg acgcgcatct tcgtcggcag cgaggggacg ctgggcatca tcaccgaagt
     2281 cagcgtgcgc gtgtacccgc agccggaggc ggtgtcggcg gcagtgtgct ctttcccgag
     2341 cacggcagac gcggtcaacg ccgtgatcca gacgatccag atgggggtgc cggtcgcgcg
     2401 cgtggagttc ctcgacgaga acagcgtgcg tgcgatcaat gcgcacgaca agctgacgct
     2461 gccggaaaaa ccgctgttgc tgttcgagtt ccacggcagc gagcatgggg tgcaggaaca
     2521 ggcgcagatc gtgcaggaca tcgcaggcga gcacaatgcg atcggcttcg agtgggcaac
     2581 ccggccggaa gaccgctcgc gcttgtggac ggcccgccac aacgcctact tcgcgctgtt
     2641 gcagatgcgc ccgggcggcc gcgcgatcac caccgattgc tgcgtgccga tctcgcgcct
     2701 ggccgaatgc atcctcgaca cgcaggcgga ctgcgagcac aacggcatga tctacggcat
     2761 tgtcggtcac gtcggcgacg gcaatttcca tgtgctgatg atgatcgatc cgagcgatcc
     2821 cgccgacatc gcgaaggcgg aaagcgtgaa cgcgcgtatc gtcgcgcgcg cgatcgcgat
     2881 ggacggcacc tgcaccggcg agcatggcgt gggcctgcac aagatggatt tcctggtgca
     2941 ggagcatggc gaggaggcga tcgaaacgat gcgcatgctg aagcgcgctt ttgatcccga
     3001 gaacatattg aaccccggta aaatcgttca tctcgactga acccccggcc cattgccggg
     3061 cgcacgcacc tcagcttgct atggccatcc gtcttcctcc ggttcatgcg ctgtccgcct
     3121 tcgaggcggc ggctcgtctc ggctcctttg cgctggcagc cgacgagctg tgcatcacgc
     3181 cttcggcgct gtcgcaccgc atccgcctgc tcgaagacct ggtcggcgag cgcctgttcg
     3241 cccgcgacgg acgtgcggtc agcctcaccg agttcgggcg ccgctacctg gacgtggtgc
     3301 gcggcgcact gcgcgcgctc accgagtttc cgctcccgca caagagcgcg ccggtgcagc
     3361 cgcgcgtgaa gatcaccgtc ccgcccacct tcgcgcgctg cttgctggtg ccgcgcctgg
     3421 cggagttcac ggctcgccat ccggagatcg tgatcgagct gttcctgtcg gtcccgctgt
     3481 acgacctcag cctgtccgaa agcgatgtcg aggtgcgttt cggcgcaggc aactatccgg
     3541 acctggtcac ggaaaagctg ttcgaggaac cgacgtttgc ggtcgccagt ccggcctatc
     3601 tagccaaggt cggcccgatc gacacgccgg ccgacctgaa gaaggcaacg ctgctgcgct
     3661 cggcgctgga accctggcag ccctggttcg aggtggccgg cctcgactgg ccggagccga
     3721 ccacgggcct gcggctcgat gacctcggcc tgctgctgga agcggtgcgc cacgggcatg
     3781 gcgtcgggtt gacgcggaaa cacttcgcgc agaagcacat cgagagcggc gagctggtgc
     3841 atctgtttcc cgacatccag ctcagtgccc cgccgcatgc ctactacgtg gtttatggcc
     3901 ggcaggtccg cgagcggccg gaggtcgatg cgtttatcgc gtggttgcgc ggggccttcg
     3961 ctccgttgac cacgtcgtga aaagaattca gggtcagaag gaaagctttt cagcaggctg
     4021 aacctcggct tgccccaatt gccacggcgt tttcgctaca ctcaccgctg ccattccctc
     4081 ccggcgctgc gccggccttc attcgcaggg acccatcatg aacgcccccg tttctccgac
     4141 taccgcattc acgccggcgc gccagcgcga ggtcgtggac gccttgcgcc gcgtactgcc
     4201 ggccgactgc gtgctgtacg acgaagagga cacgcgccct tacgagtgcg acggcctctc
     4261 ggcctatcgc cagctgccga tggtggtcgc gctgccgcgc gacgagcagc aggtgatcga
     4321 cgcactgaaa acctgccggg cgctgaacgt gccgattgtg ccgcgcggcg cgggtaccgg
     4381 cctgtccggc ggcgcgatgc cgatatccga tggcgtggtg ctgtccacgg cccggctcaa
     4441 ccgcgtgctg aagctcgatc cgtatgcccg catcgcgatc gtgcagccgg gtgtgcgcaa
     4501 tctcgcgatc tcggatgccg ccaatgccca cggcctgtac tacgcacccg atccttcgtc
     4561 gcagatcgcc tgcacgatag gtggcaatgt cgcggaaaac tccggcggcg tgcactgcct
     4621 gaaatacggg ctgaccatcc acaacgtgct gcgcgtacgc atggtgacga tcgagggtga
     4681 cgtggtcgaa ctcggcagcg aggcactcga cgcgccggga ctggacctgc tggcagtgtt
     4741 catcggctcc gagggcatgc tcggcgtggt gaccgaggtg actgtcaagc tggtgcccaa
     4801 gccgcagcag gcgcgcgtga tcatggcgtc gttcgacaac gtggtgaagg ggggcgacgc
     4861 ggtggccagc gtgattgccg ccggcatcat tccggccggc ctggaaatga tggacaagac
     4921 ttcctcgcgc atggtcgagc ccttcgtcca tgccggctat gacaccgagg ccgaggcgat
     4981 cctgctgtgc gagtccgacg gcgttcccga ggaagtggac gaggaaatcg cgcgcatgtc
     5041 ggacgtgctg cgcgcatccg gtgccaccgc gatctcggta tcgcagaacg aggccgagcg
     5101 cgcacgcttc tggtccgggc gcaagaacgc gtttccggct gccggccgca tctcgcccga
     5161 ttactactgc atggacggca cgatcccgcg caagcgcctg gccgaagtcc tgctcgcgat
     5221 cgccgagatg gagaacaagc acggcctgcg ctgcgcgaac gtgttccacg ccggggatgg
     5281 caacctgcat ccgctgatcc tgttcgatgc gaaccagccg ggcgaattcg agcgtgccga
     5341 agccttcggc gccgacattc tcgaactgtg cgtaaaggtc ggcggcacca tcaccggcga
     5401 gcatggcgtg ggcatcgaga agatcaactc gatgtgcgtg cagttctcgc cgcaggagct
     5461 cgaagccttc ttcgccgtca agcgtgcgtt cgataccgct ttcctcctca acccggacaa
     5521 ggccatcccg acgctgaacc gctgcgccga gtttggcagg atgcgcgtct cgggcggcca
     5581 gctgaaattc cccaatctgc cgcgcttctg agatggattc gatactccag caattccgcg
     5641 acaccgtggc gtcggccagt gtcggcaaac agccgttgcg catccgcggc ggcggaacca
     5701 aggattggta cggccagtcg ctcgcgggcg aggtgctcga cacccgcgcc tacagcggca
     5761 tcctcgcgta cgaaccgacc gagctggtga tcactgcgcg cagcggcacg ccgctcgtcg
     5821 agatcgaggc cgcccttgcc gaacgcaagc agatgctggc gtttgagccg ccgcgcttcg
     5881 gcccggcgtc gacaataggc ggcgtggtgg ccgccgggct gtccgggccg cgccggcagg
     5941 cggttggcgc agtacgcgat ttcgtgctcg gtgcgaccct gatggactgc aagggcgagg
     6001 tgctgcgctt cggcggccag gtgatgaaga acgttgcggg ctacgatgtc tcgcgcctgc
     6061 tcgcgggctc gctcggcacg ctcggactga tcctcgatgt ctcgctgaag gtgctgccgc
     6121 gtccgttcgc cgagattacg ctgcgcttcg agatgcgcaa aaccgaggcg atcgaacagt
     6181 tgaaccgctg gggcggccag ccgttgccga tctcggccag tgcctggcac ggcggcgtgc
     6241 tggtcgtgcg cctttccggt cccgagccgg cgctcagagc cgcgcagcag aagatgggcg
     6301 gtgaagtcgt cgaggatgcc gagccgctgt ggcagggtct gcgcgaccag agcaatgcat
     6361 attttggcga agggacactc tggcgcctgt ccgttccttc aaccacgccg ccgctcgcgc
     6421 tcaagggcga acacctgatc gagtggggcg gcgcgcagcg ctggctgcgc agcgatgtcg
     6481 atgccgcgac catacgccat gccgccgcga ccgtcggcgg ccacgcgacg ctgatgcgcg
     6541 gcgacaaggg cgtcggcgtg ttccacccgc tgccgcctgc cgtcatgcgc atccaccgca
     6601 acctgaagac cgctttcgat ccgtccggaa tattcaatcc ggggcggatg tacccagagt
     6661 tctgaacatg caaaccaatc tcgcggattt catcaagcac accgccgaag gcgaggaagc
     6721 cgacgcgatc ctgcgcaagt gcgtgcactg cggcttctgc accgcgacct gcccgaccta
     6781 ccagctgctc ggcgacgagc tggacggccc gcgcgggcgc atctacctga tgaaacaggt
     6841 gctggagggc aaggagccga cgatggccac gctgacccac ctggaccgct gcctgacctg
     6901 ccgcaactgc gagaccacgt gtccgtccgg cgtgcagtac ggtcggctgg tcgatatcgg
     6961 ccgcaaggtg gtggaggcgc gcgtcgagcg gccgctggcg cagcgcgcgg tgcgctatgc
     7021 gctgaaggag gcgctgccgc gcaagtggct gttcggtccg gcgatgaagg ccgggcagat
     7081 cgtgcggccg ctgctgccgc aggtgctgaa gaacaaggtg ccggcgccgc agcaggccgg
     7141 cgcatggccg cgcgcggcgc atgcgcgcaa gatgctgctg ctcgacggct gcgtgcaacc
     7201 ggcgatggcg ccgaatatca acagcgcgac cgcgcgcgtg ctggacacgc tcggcgtgca
     7261 gctggtggtg cccaggggca ccggctgctg cggcgcgatc cggtatcaca tgaacgacca
     7321 agccggcggg cttgacgaca tgcgccgcaa cgtcgacgcc tggtggccgc atgtggaagc
     7381 cggcgtcgag gccatcgtga tgaacgcgtc cggctgcgga gtgacggtca aggag
//
LOCUS       DATLGF010000449         5653 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_233739, whole genome shotgun sequence.
ACCESSION   DATLGF010000449 DATLGF010000000
VERSION     DATLGF010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233739"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /gene="nuoL"
                     /locus_tag="VM406_13725"
     CDS             complement(<1..1295)
                     /gene="nuoL"
                     /locus_tag="VM406_13725"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HVL77068.1"
                     /translation="MAGQLNANLLLAIPLAPLVGAAIAGLFGTRFFGNVIGRTASHTV
                     TILGVLISFLLSAHTLWQVINGAPVFNETLYTWMTVMGMPLEIGFLVDSLTAMMMVVV
                     TFVSLMVHIYTIGYMADDEGYNRFFSYISLFTFSMLMLVMSNNFLQLFFGWEAVGLVS
                     YLLIGFWFKRPTAIYANMKAFLVNRVGDFGFVLGIGLLLAYSGSLSYADVFAARDNLA
                     TIGFPGTDWHMLTVACICLFIGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTA
                     GIFMVARMSPLFELSDTALSFVLIIGAITALFMGFLGIIQTDIKRVVAYSTLSQLGYM
                     TVALGASAYQVAVFHLMTHAFFKALLFLGSGAVIHALHGEQDIRNMGGLKKYLPVTYW
                     TFLIGSLAIAGVPLLSGFFSKDEILFETFSRGYQLLWAMG"
     gene            complement(1324..1632)
                     /gene="nuoK"
                     /locus_tag="VM406_13730"
     CDS             complement(1324..1632)
                     /gene="nuoK"
                     /locus_tag="VM406_13730"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HVL77069.1"
                     /translation="MELTLSHFLILGAILFAISIIGIFLNRKNIIVLLMAIELMLLAV
                     NMNFVAFSYYLGDAAGQIFVFFILTVAAAESAIGLAILVVLFRNLDTINVQDLDRLKG
                     "
     gene            complement(1645..2289)
                     /locus_tag="VM406_13735"
     CDS             complement(1645..2289)
                     /locus_tag="VM406_13735"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HVL77070.1"
                     /translation="MELKTALFFAFAAVLVFAALRVVTDRNPVHAALYLVLSFFSAAA
                     LWLLLKAEFLAIVLVLVYVGAVMVLFLFVVMMVDIDLAEIRSGFMRYLPLAALVGILI
                     VLQMGAVLWHGFYATEADVPEASAYIGTTAELGRVLYTDYIYAFEIAGILLLVALVAA
                     AGLTLRRRKDTRYFDPGEAVKVKRADRIRLVKMKAESERTNANEEANASTTEKQ"
     gene            complement(2329..2817)
                     /gene="nuoI"
                     /locus_tag="VM406_13740"
     CDS             complement(2329..2817)
                     /gene="nuoI"
                     /locus_tag="VM406_13740"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HVL77071.1"
                     /translation="MQAIRDFLGSLMLRELLKGLALTGRYLFARKITVQFPEEKTPLS
                     PRFRGLHALRRYPNGEERCIACKLCEAVCPAMAITIESAEREDGTRRTTRYDIDLTKC
                     IFCGFCEESCPVDSIVETNILEYHGEKRGDLYFTKEMLLAIGDRYEKDIAAARSVDAA
                     YR"
     gene            complement(2836..3903)
                     /gene="nuoH"
                     /locus_tag="VM406_13745"
     CDS             complement(2836..3903)
                     /gene="nuoH"
                     /locus_tag="VM406_13745"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HVL77072.1"
                     /translation="MDGMLTSLNTAGAGMFGGAWPLVWTMLGILAILLPLMGAVAYLT
                     LWERKLIGWMHIRVGPNRVGPLGLLQPIADAVKLLTKEIVVPMRANKALFVIAPIMTI
                     MPALAAWSVIPFGPEVVLANINAGLLFIMAITSLEVYGVIIAGWASNSKYAFLGAMRA
                     SAQMVSYEIAMGFALVVVLMVSGSLNLIEIVTVQTQGYFADLGLNFLSWNWLPLLPMF
                     VIYVVSGIAETNRHPFDVVEGESEIVAGHMVEYSGMSFAMFFLAEYANMWLFSILAVL
                     LFLGGWSAPVAFLDFIPGWIWLGLKTFFMVSLFIWVRASFPRYRYDQIMRLGWKVFIP
                     LSVAYLIIVAIWMQTPWNIWP"
     gene            complement(3904..>5653)
                     /locus_tag="VM406_13750"
     CDS             complement(3904..>5653)
                     /locus_tag="VM406_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HVL77073.1"
                     /translation="CPVGALTSKPFRYAARTWELSRRKSVSPHDSLGSNLIVQVKGGK
                     VLRVLPLENEAVNECWLSDRDRFSYDALNSDERLTVPMVKQDGKWIETDWQTALEYVA
                     HGLKDITGKHGTDALAALAAPYSTLEEFALLGKLVRGLGSENIDFRLRQSDFALDGKI
                     TPWLGMPIEQMGTLKGALLIGSFLRKDHPLLAARLRTCAKRGAQINVLHASDEDLLMP
                     IANKLIAPPSSWLALLNEVAVGIAAEKGVAAPAGLEGVTVSDAAKEVAKNLLSDDERA
                     VLLGSAAIHHPQASELHAVAEWIATALGAHFGYLTEGGNALGGYLAGALPGKGGNALQ
                     AFAQPRKAYMLLHAEPELDCYDPQAARAALNGADMVVVMSPYKHGMDYADVLLPIAPF
                     TETAGTFVNCEGRAQGFNGSAKPLGETRPAWKVLRVLGNLLGLSGFDYEASEDIRAEV
                     LGTRKAEEADLSASLNNIARLPLQSAKAGTGEALERLADVPIYHSDAIVRRSPPLQQT
                     SHAKPPKAWLSAELARHLGVADDQPVRVRQGQGEAILPAGIDPKLPANVVKVSAAHPT
                     TATLGGMFGSVIVEKA"
BASE COUNT         1172 a         1779 c         1744 g          958 t
ORIGIN      
        1 cccatcgccc acagcagctg atacccgcgc gagaacgtct cgaacaggat ctcgtccttc
       61 gagaagaacc ccgagagcag cggcacgccc gcgatcgcca gcgatccgat caggaacgtc
      121 cagtaggtga ccggcaggta cttcttcagg ccgcccatgt tccggatgtc ctgctcgccg
      181 tggagcgcgt ggatgacggc gcccgagccg aggaacagca gcgccttgaa gaacgcgtgc
      241 gtcattaggt ggaacaccgc gacttggtaa gccgacgcgc ccagcgcgac ggtcatgtaa
      301 ccgagctgag acagcgtcga gtacgccacc acacgcttga tatcggtctg gatgatgccc
      361 aggaagccca tgaacagcgc ggtaatggcg ccgatgatca gcacgaacga cagcgcggtg
      421 tccgacagtt cgaacagcgg cgacatgcgc gcgaccatga agataccggc ggtcaccatg
      481 gtggccgcgt gaatcagtgc ggagatcggg gtcgggcctt ccatcgagtc cggcagccac
      541 acgtgcagcg ggaactgcgc cgatttaccc atcgcgccga taaacaagca gatacaagcg
      601 accgtcagca tgtgccagtc ggtacccggg aagccaatcg tggccaggtt gtcgcgggca
      661 gcgaacacat cggcgtagct caggctaccg ctgtacgcca gcaacaggcc gataccgagc
      721 acgaagccga agtcacccac gcggttgacc aggaacgctt tcatgttggc gtagatggcc
      781 gtcggacgct tgaaccagaa gccgatcagc aggtacgaca ccaaacccac cgcctcccag
      841 ccgaagaaca gctgcaggaa attgttgctc atgacgagca tgagcatcga gaacgtgaac
      901 aacgagatat aggagaagaa gcggttgtag ccttcgtcgt cggccatgta gccgatcgtg
      961 tagatgtgca ccatcagcga gacaaaggtc accaccacca tcatcatcgc ggtgaggctg
     1021 tcgaccagga agccgatctc cagcggcatg cccatcaccg tcatccaggt gtacaacgtt
     1081 tcgttgaaca ccggcgcacc gttgataacc tgccagaggg tgtgcgcgga cagcaggaag
     1141 gaaatcagca cgccgagtat cgtcacggta tgcgacgcgg tgcggccgat gacattgccg
     1201 aagaacctgg ttccgaacag accggcgatt gcagcgccga ccaaaggcgc cagcggaatt
     1261 gcgagcagta gattagcgtt gagttgcccc gccatgatgg accttatcgt tgttattcag
     1321 tgattaaccc ttgagacgat ccagatcctg cacattgatg gtgtccaggt tgcggaacag
     1381 caccaccagg atggcaagac cgatcgccga ctccgcggca gccactgtga gaatgaagaa
     1441 aacgaaaatc tgccctgcgg cgtcgccaag gtaatacgag aacgcgacga agttcatgtt
     1501 cactgccagc agcatcagtt cgatggccat cagcaggaca atgatgttct tgcggttcaa
     1561 gaaaataccg atgatcgaga tcgcgaacag gatcgcgccc aggatcagaa agtgcgaaag
     1621 cgtcagctcc acttgcgatt ccccttattg cttttctgtc gtggaggcgt tcgcctcttc
     1681 gttggcgttc gtccgctccg attctgcctt catcttcacc aggcgaatgc ggtctgcgcg
     1741 cttgaccttg acggcctcgc ccggatcgaa gtagcgggta tccttgcggc ggcgcagggt
     1801 gagaccggcc gccgcaacca gtgccaccag cagcaggatg ccagcaatct cgaacgcgta
     1861 gatgtagtcg gtgtacagca ccctgccaag ttcggcagtg gtgccaatat aggcggatgc
     1921 ctcgggcaca tcggcttcgg tcgcgtaaaa gccgtgccag agcaccgcac ccatctgcag
     1981 gacgatcagt atcccaacca gtgcagccag cggaagatag cgcatgaacc cgctgcgtat
     2041 ctcggcaaga tcgatgtcca ccatcatcac gacgaacagg aacagcacca tcacggcgcc
     2101 gacgtagacg agcacaagca cgatggccag gaactccgcc ttgagcagca gccagagcgc
     2161 cgcggcggag aagaaggaca gcaccagata caacgcggcg tgaaccggat tgcggtcggt
     2221 gaccacgcgc agcgcggcga acacgagtac cgcggcaaac gcgaaaaaca aggcagtttt
     2281 caattccata gtcgaccagt cattcagctg ttctgtggcg ccatcgcatc agcggtaggc
     2341 ggcatcgacg gagcgtgccg ccgcgatgtc cttctcgtag cgatcgccga tcgcgagcag
     2401 catctccttg gtgaaataga gatcgccgcg cttttcaccg tggtactcga ggatgttggt
     2461 ttccacgatc gaatccaccg ggcaggactc ttcgcagaag ccgcagaaaa tgcatttggt
     2521 caggtcgatg tcatagcgcg tggtgcggcg ggtaccgtcc tcgcgttcgg ccgattcaat
     2581 ggtaattgcc atggccgggc agaccgcttc gcacagcttg caggcgatgc agcgctcttc
     2641 gccgttcgga tagcggcgca gcgcgtgcag cccgcggaaa cgcggcgaca acggcgtctt
     2701 ctcttccgga aactgcacag tgatcttgcg ggcgaacaga tagcgcccgg tcagggcgag
     2761 ccccttgagg agctcgcgca gcatcaagct gcctaggaaa tctcgtattg cttgcatgtg
     2821 ttcaggcctc tcgcattatg gccagatgtt ccagggcgtc tgcatccaga tcgccacgat
     2881 gatcaggtat gcgaccgaca gcggaatgaa caccttccag ccgaggcgca tgatttggtc
     2941 atagcggtag cgcgggaacg aggcgcgcac ccagatgaac agcgacacca tgaagaaagt
     3001 cttgaggccg agccagatcc agcccggaat gaagtcgagg aacgcgaccg gcgccgacca
     3061 gccgcccagg aacagcagca cggccagaat cgagaacagc cacatgttcg cgtactcggc
     3121 gaggaagaac atcgcgaacg acatgcccga gtactcgacc atgtgtccgg cgacgatttc
     3181 cgattcgccc tcgaccacgt cgaacgggtg gcgattggtt tccgcaatgc ccgacacgac
     3241 gtagatcacg aacatcggca acagtggcag ccagttccac gacaggaagt tcagcccgag
     3301 gtcggcgaag tagccttgcg tctgcacggt gacgatctcg atcaggttca ggctgccgga
     3361 gaccatcagc acgaccacca gcgcgaagcc catcgcgatt tcgtaggaca ccatctgcgc
     3421 ggatgcgcgc atcgcgccga ggaaggcgta cttcgagttc gacgcccagc cggcgatgat
     3481 gacgccatag acttcgagcg aggtgatcgc catgatgaaa aggaggccgg cgttgatgtt
     3541 cgccagcacg acctccgggc cgaacgggat caccgaccag gcagccagcg caggcatgat
     3601 ggtcatgatc ggcgcaatca cgaacaaggc cttgttcgcg cgcatcggca ccacgatctc
     3661 cttggtgagc agcttgaccg cgtcggcgat cggctgcagc aggcccagcg gccccacccg
     3721 gttcgggccc acgcggatat gcatccatcc gatgagcttg cgctcccaca gtgtcagata
     3781 ggcgaccgcg cccatcagcg gcagcaggat cgcgagaatg cccagcatgg tccagaccag
     3841 cggccaggca cccccgaaca ttccggctcc ggcggtatta aggcttgtaa gcatgccgtc
     3901 catttatgct ttctccacga tcacggaacc gaacatcccg cccagcgttg ccgtcgtcgg
     3961 atgggctgcc gaaaccttca ccacgttcgc gggcagcttg ggatcgatcc cggcgggcag
     4021 gatcgcctcg ccctgccctt gcctcacgcg taccggctga tcgtccgcca cccccagatg
     4081 cctcgcaagc tcggcagaca gccatgcctt cggcggcttc gcgtgcgagg tctgctgcag
     4141 cggcggggaa cgccgcacga tcgcatcgct gtgatagatc ggcacgtcgg ccagacgctc
     4201 gagcgcttcg ccggtgcccg ccttggcgga ctggagcggc aggcgggcga tgttgttcag
     4261 gcttgccgac aggtcggcct cttcggcctt ccgggtgccc agcacctcgg cgcggatgtc
     4321 ttccgacgct tcatagtcga agccggagag ccccaacagg ttgccaagta cgcgcaggac
     4381 cttccacgca ggtcgggtct cgccgagcgg cttggcgctc ccgttgaagc cctgggcacg
     4441 gccctcgcag ttcacgaagg tgccggcggt ctcggtaaag ggggcgatcg gcaacaacac
     4501 atcggcgtaa tccatgccgt gcttgtacgg cgacatcacc accaccatgt ccgcaccgtt
     4561 caatgccgca cgcgccgcct gcgggtcata gcaatcgagc tcgggctccg cgtgcagcag
     4621 catgtaggcc ttgcgcggtt gcgcgaacgc ctgcagcgca ttgccgccct tgccgggcag
     4681 cgcgccggcg agatagccgc cgagtgcgtt gccgccttcg gtcaggtagc caaaatgcgc
     4741 gcccagcgcg gtagcgatcc attcggccac ggcgtgcagt tccgaggcct gcggatggtg
     4801 gatcgcggcg ctgccgagca gcacggcgcg ttcgtcgtcc gacagcaggt tcttcgcgac
     4861 ttccttcgcc gcatcggaga cggtgacgcc ctcgagaccg gccggtgccg ccacgccctt
     4921 ctctgcggcg atgcccacgg caacctcgtt caacagggcg agccaggacg aaggcggcgc
     4981 aatcagcttg tttgcgatcg gcatcagcag gtcttcgtca ctggcatgca gcacgttgat
     5041 ctgcgcgccg cgctttgcgc aggtgcgcag gcgggcagcc aacagcggat ggtccttgcg
     5101 caggaaggaa ccgatcagca gcgcgccctt gagcgtgccc atctgctcga tcggcatacc
     5161 cagccacggc gtgatcttgc cgtcgagtgc aaagtcggac tggcgcaggc ggaagtcgat
     5221 gttctccgat ccgaggccgc gtaccagctt gcccagcagc gcaaactcct ccagcgtgga
     5281 atacggggcg gcgagcgcgg cgagtgcgtc tgttccgtgt ttgccggtga tgtccttcag
     5341 gccatgcgcc acatactcga gcgcggtctg ccagtcggtt tcgatccact tgccgtcctg
     5401 cttgaccatc ggcaccgtca ggcgctcgtc gctgttgagc gcgtcgtacg agaagcggtc
     5461 gcggtcggac agccagcatt cgttgacggc ctcattctcc agcggcagta cgcgcagcac
     5521 cttgccgccc ttgacctgca cgatcaggtt cgagccgagg ctgtcatgcg gactcaccga
     5581 cttgcggcgg gacagctccc aggtacgcgc ggcgtagcgg aacggcttgg aggtcagcgc
     5641 gccgaccggg cac
//
LOCUS       DATLGF010000450        11540 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_154402, whole genome shotgun sequence.
ACCESSION   DATLGF010000450 DATLGF010000000
VERSION     DATLGF010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 11540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11540
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154402"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VM406_13755"
     CDS             <1..448
                     /locus_tag="VM406_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77074.1"
                     /translation="AHLFLLYFAVLSAMTPPVAVAAYAASAISGRNPLGIAVKSCRLA
                     MAAFVLPFAIIYDPGILMGGSWSETLAGMGSALAAIVLISIAAEGYLKRPLSVPVRLL
                     GGAAGLAYLAPGATGVVIGSGLLATAVVAMLLTRTAEAPMLTPKED"
     gene            451..2823
                     /locus_tag="VM406_13760"
     CDS             451..2823
                     /locus_tag="VM406_13760"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HVL77075.1"
                     /translation="MTKVLLQESGHASALSGQAQAASRKQRYIGKSVNRLEDFPLVTG
                     RACFVGDVSFPNQLHMRVVRSPVAHGRIVAIDASDALQAPGVVAVWTAADIAAIPPIP
                     FRATKVQGLLPYRQPVLAKELVRYVGEPVAVVFADDPYRAEDAAELVAVDIEPLDPIV
                     TATAEPGEFENGIDTEPVVLSKGYGNVEQAMAAAAAVIELELSIGRHSGIPMETRGAL
                     ARYDQARDHLELYGAAKRPHWNRDQLAEMLDRAPSTIDLFENHVGGGFGIRGELYPED
                     VLVCVGALRLRRPVKWIEDRREHMMCANQSRQQTHRVKAAVDGEGRLLAIDEQLFHDQ
                     GAYVRTHGARVADMTIGLLLGPYRVPAYRAAAHYRLTNKTPAATYRSPGRFEGSFVRE
                     RLMDTIAHRLGLDPIEVRRRNLIGKEEMPFARNLDALETEVVLDSGDYAGLLDKTLAA
                     LDWPQLRAQIQARRAAGECVGLGIGMFVEKSGLGPYDGVRIHVDTRGRVEVVTGAASI
                     GQGVETVVAQICAETLGVDYRDIRVIHGQTNRIAYGFGAHASRVTVMTGEATRIAAVN
                     LRDKALDVAAQMLKLPAASLDIVDGKVVTADGTSMLALGEIARQLIPASPVRGEREPG
                     LSAEGWFHSDHMNYPYGVHVAQVRVDEETGGITVERYMVSYDVGRAVNPMLIEGQIQG
                     GLAQGMGGALFEEFLYDDQGQPLSVTFADYLMPTSKEMPALEVLVTEDAPSPLNPLGI
                     KGAGEGGVTAAGAAIASAIDDAICIPGAIRRLPVTPQRLMQILKGADNRA"
     gene            2816..5239
                     /locus_tag="VM406_13765"
     CDS             2816..5239
                     /locus_tag="VM406_13765"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HVL77076.1"
                     /translation="MRDKSDKSAKPIFGASTPRLEDDALVRGKGRYLDDLAAPGALAA
                     YFVRSPYAHAAIGAIDADAAREVPGVVAIYTLDELRAHLTDTQLVVGLPTKAYLLDVN
                     RPVLAREEATYVGEPVAVIIADTRHAAEDAAELLEIDYDPLPAVSDCRSALAADAPTV
                     QSKLPHNLVAAFGFDYGDVDDAFAGAAHVFRESLWVHRGGSHSLEGRGVLAEYSAASN
                     CLTVWMSTQTPHTARRMLCDLLGRSDDGVRVITPDVGGGFGPKLVFYSEEVVVALAAM
                     LLGRPVKWVEDRREHFVATTQERDQYWDVEIAVDADARILGVRGALLHDHGAYTARGV
                     NVTYGSAVTVPLPYNVPAYRMDVSMALTNKVPVTPVRGAGQPQAVFVMERLLDRVARE
                     LSLDGAEVRRRNLVRPEQMPCEKPLVLRGGRNVLLDSGDFIATQAQALELAGWKSFRE
                     RQQQALEEGRYIGMGLANYVEGTGRGPYESVRVRIAGNGKVLVSSGAAAMGQSTKTML
                     AQVVAEQLGRDMDNIVVTTGDTQAVALGFGGFNSRQTVTAGASAHAAALKLRARVFEV
                     AADLLKADPAELEIAGTRVFAKTRPEQALGLGQIARAAAGLPGFILPGGNGPALEVLE
                     HVIIDDMSYSNGSAVVEVEVDVETGGVKLNRVVLAHDCGRMVNPMIVDGQVIGGIAHG
                     LGNALFEWMGFDAQAQPITTTFADYLLISAAEMPAVEMIHQESPSPLNALGVKGVGES
                     GVIPMPAAVASAIEDALRPLGVHIDRAPLSPMALRALIRGGRGEDVEHDWRADSDRIR
                     AAHAGAAHG"
     gene            5232..5969
                     /locus_tag="VM406_13770"
     CDS             5232..5969
                     /locus_tag="VM406_13770"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HVL77077.1"
                     /translation="MDKALHELGEELALAHHILIDQGVLEAFGHASARLPGDPGRFML
                     PAAGAPSRMRAADMLVFGMDGEPVAPTERALFSERFIHAAIYRARPDVMAICHHHSPS
                     IMPFCVSGVPLRPVSQTGASMGPQVPFWDSRSEFGDTKLLVTTDAEADSLARALGPAW
                     MVLMRRHGATVVGTSVKELVFRAVHYCRDADHQLRAAALGTIDPLTRAECSLAGQLRA
                     EPVARCWEHWKALLPAQLIERLTGTAE"
     gene            5966..6832
                     /locus_tag="VM406_13775"
     CDS             5966..6832
                     /locus_tag="VM406_13775"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HVL77078.1"
                     /translation="MKPAPFTYHRPASVDEALAILADVAEEGGRVIAGGQSLIPMMAL
                     RVAYPPHLIDINEIAELAQTSVSGTTFSIGALTRHARFHEPVAPGPLGPLMSAVAHHI
                     AHYPIRQRGTFCGSLAHADPASEWCLVTATLDGVIVVRNKNGMREIPVGEYFQGVMAT
                     AVEPEEMLVEVRLPLLADDERFGFVEFNRRAGDFALGMSLVTLRIAEGVIASARVGLG
                     GVEEHPRRIAEAEAELVGRAPTDVVFRKAAAAAAAVVEPLEDAQTSADYRRDLTAVVI
                     RRALEQAMRDGR"
     gene            6853..7362
                     /locus_tag="VM406_13780"
     CDS             6853..7362
                     /locus_tag="VM406_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HVL77079.1"
                     /translation="MTDKTDINLDVNGGSFAVRVEPRKTLADTLREDCGLTGTHVGCE
                     HGVCGACTVLVDGQPVRSCLMFAVQAQGHEVRTVESLADGDTLNRLQQAFCDNHALQC
                     GFCTPGFLMLITGALEKNPDMTDDEIVEVLSSNLCRCTGYINIVKAVKQVRDMGKAAA
                     PAAGAAQAA"
     gene            7433..7906
                     /locus_tag="VM406_13785"
     CDS             7433..7906
                     /locus_tag="VM406_13785"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HVL77080.1"
                     /translation="MEMSGSERIGASREAVYAALNDPEILKQALPGCQELEKVSDTEF
                     NAVIVVKVGVIKATFKMAGTLMDLDPPNGYTLKGEGKGGVAGFAKGSAQVRLEEDGDA
                     TILHYTVAADIGGKLAQLGARLINSTAMKFAGEFFGNFSRIVAGEGAAPAQEAAA"
     gene            7936..9639
                     /locus_tag="VM406_13790"
     CDS             7936..9639
                     /locus_tag="VM406_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HVL77081.1"
                     /translation="MTNTILTTLSPQRLKQHYEEGFWQSDTIYMLARKNAQAHPDHYA
                     LRDRYRRLTFAQAVEAADRLAAHLAAQGVRRGQRVAVWLSSRLETAIALLACSRNGYV
                     CCPSLHRNHTVGEIGELMERMRAAALVAEIGYGADADRHDVFALAEAQKTMRAIYRVP
                     ALAPGDAQHLPFGPLLATGAGAADATPFDTEPDTLVYLAFTSGTTGKPKGVMHSDNTL
                     LANARALAGDWSLGPGSVIYTLSPLSHNLGFGAMVAAFAVGAELVVHDTQRGESLAER
                     LAETGATFMFGVPVHAIDLLKELRERDAALVKTVKGFRISGAPVPQEVAAELLQYGVV
                     PQSGYGMTEACSHHYTLPTDDPKMIIETSGRSCPGYEIRIFTRDDPEKEAPVGEVGQI
                     GGRGASLMLGYYDNQEATEDSFNAQGWFMTGDLGWVDAEGYLRITGRKKDVIIRGGHN
                     IYPAKIEALAGRHAAVERAAAVPVADERMGEKVCLVLMPRAGKLVDPMDMLAHLDATG
                     LSKFDMPEYMVLVDDIPLTASGKILKRDLVDQIKAGTLVPQPVRWAGPKASAAVAEAV
                     Q"
     gene            9636..10049
                     /locus_tag="VM406_13795"
     CDS             9636..10049
                     /locus_tag="VM406_13795"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HVL77082.1"
                     /translation="MSEQAQALARLEEEMRRPPFHAVLAPQAHAVDPESGEVVIRLPF
                     HPDFGRDPVHVGFHGGVIASLIDLAGHAAVAVKVGKPTPTVDLRIDYLRPAPGVTLEA
                     RARVLRAGRAIARADIDVLADGVLVAVGRGTFSTL"
     gene            10080..11075
                     /locus_tag="VM406_13800"
     CDS             10080..11075
                     /locus_tag="VM406_13800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HVL77083.1"
                     /translation="MRAVIINEFGNIDTPVYGEMPTPRPGPGEVVIRVRATAVNFVDL
                     LVIGGKYQFLPERPFTPGKGPAGVVAAVGEGVASLKVGDRVLAMAEHGGYGEQALVAA
                     NQCYPLPAGMSFAEAASMSLAYDTAWFALRERGRYKEGETVLVLGASGGVGYAAVQLA
                     KAMGAKVLAGVSSPSKADMVRAAGADALIDLSAGNLRDSLREQVYAATDGKGADVVID
                     MLGDDIFDAALRAVAWRGRIVVVGFAAGRIPTIKANYLLVKNIEASGLQISDYRKRMP
                     GLVAECFAEIFAFYEAGKVRPAPYTTLPLSEFRDGLRMVQDRTAKGRVVLVQDDE"
     gene            11094..>11540
                     /locus_tag="VM406_13805"
     CDS             11094..>11540
                     /locus_tag="VM406_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HVL77084.1"
                     /translation="MTMPLPTHNRYPFSLIDQRKDFSWPDGKRLAFYVCNNIEYFAFG
                     AGLGSEIGMVNPPPNHRSYAWRDYGNRVGLSRILDLLDELGLPGAHNVNSFLYDAHPE
                     IFERIRKRGDEIVGHGRTNAERQSSMWEADEARLIREATDAIAKHEG"
BASE COUNT         1897 a         3927 c         3975 g         1741 t
ORIGIN      
        1 ggcgcacctg ttcctgctgt acttcgcggt gctgtcggcg atgacgccgc cggtggcggt
       61 cgccgcctat gccgcgtcgg ccatttcggg ccgcaatccg ctcggcatcg cggtcaagtc
      121 ctgccggttg gcgatggccg ccttcgtgct gccgttcgcc atcatctacg acccgggcat
      181 cctgatgggc ggctcctgga gcgaaacgct ggcgggcatg ggctcggcgc tagccgcgat
      241 cgtgctgatt tcgatcgccg cggaaggcta tctgaagcgt ccgctgtcgg ttccggtacg
      301 cctgctcggc ggcgcagccg gcctcgccta tctcgcgcca ggcgccaccg gcgtagtgat
      361 cggttccggc ctgctggcga cggccgtcgt cgcgatgctg ctgacgcgca ccgccgaagc
      421 gccgatgctg acgccgaaag aagattgaag atgacgaaag tgttattgca ggaatccgga
      481 cacgcgagcg cgctgtccgg acaggcgcaa gcggcctcgc gcaagcagcg ctacatcgga
      541 aagtcggtca accggcttga agatttcccg ctggtgaccg gccgcgcctg cttcgtcggc
      601 gacgtctcgt tcccgaacca gctgcacatg cgcgtggtgc gctcgccggt cgcgcatggc
      661 cgtatcgtgg ccatcgatgc gtcggacgcg ttgcaggcgc ccggcgtggt ggcagtatgg
      721 acggcggccg acattgccgc gattccgccg attccgtttc gcgcgaccaa ggtgcagggc
      781 ctgctgccgt accggcaacc ggtgctcgcg aaggagctgg tgcgttatgt cggcgagccg
      841 gtcgcggtgg tgttcgccga cgatccatac cgcgccgaag acgcggccga gctggttgcg
      901 gtcgacatcg aaccgctcga tcccatcgtc accgccaccg ccgagccggg cgagttcgag
      961 aacgggatcg acaccgagcc ggtggtgctg agcaagggct acggcaacgt cgagcaggcg
     1021 atggcggccg ccgccgcggt gatcgagctg gaactctcga tcggccgcca ctccggcatt
     1081 ccgatggaaa cccgcggcgc gcttgcgcgc tacgaccagg cgcgcgacca cctggaactg
     1141 tacggcgcgg caaagcgccc gcactggaac cgcgatcagc tggccgagat gctggaccgc
     1201 gcgccctcga ccatcgacct gttcgagaac cacgtcggcg gcggcttcgg catccgcggc
     1261 gagctgtatc ccgaggatgt gctggtatgc gtgggcgcgc tgcggctgcg ccggccggtg
     1321 aagtggatcg aggacaggcg cgagcacatg atgtgcgcga accagtcgcg ccagcagacg
     1381 catcgcgtca aggccgcggt cgacggcgaa ggccggctgc tggcgatcga cgagcaactg
     1441 ttccacgacc agggcgccta tgtgcgcacg cacggcgcgc gcgtggccga catgacgatc
     1501 ggcctgttac tcggcccgta ccgtgtgccc gcctatcgtg ccgccgcgca ctaccggctg
     1561 accaacaaga cgccggccgc gacctaccgt tcgccgggcc gcttcgaagg ctcgttcgtg
     1621 cgcgaacgcc tgatggacac catcgcacac cgcctcgggc tggacccgat cgaggtgcgc
     1681 cgacgcaatc tcatcggcaa ggaagagatg ccgttcgcgc gcaatctcga tgcgctcgaa
     1741 accgaggtgg tgctggactc cggcgactac gccgggctgc tggacaagac gttggccgcg
     1801 ctggactggc ctcagctgcg cgcgcagatc caggcgcgcc gcgccgccgg cgagtgcgtg
     1861 ggcctgggca tcggcatgtt cgtcgaaaag agcgggctcg gcccgtatga cggcgtgcgc
     1921 atccatgtag acacgcgtgg acgcgtggaa gttgtcaccg gtgccgcgtc gatcggccag
     1981 ggtgtggaaa cggtggtcgc gcagatctgc gcggagacgc tcggtgtgga ctatcgcgac
     2041 atccgcgtga tccacggcca gaccaaccgc atcgcctacg gcttcggcgc ccacgcctcg
     2101 cgcgtgaccg tgatgaccgg cgaagccacg cgtatcgccg ccgtgaacct gcgcgacaag
     2161 gcgctcgacg tggccgccca gatgctcaag ctgccggccg cttcgctcga catcgtcgac
     2221 ggcaaggtgg tgaccgccga cggcacgtcc atgcttgcgc tcggcgagat cgccaggcag
     2281 ctgatccccg cgtcgccggt gcgcggcgag cgcgagccgg gcctgtctgc cgaaggctgg
     2341 ttccacagcg accacatgaa ctatccgtac ggcgtgcatg tcgcccaggt gcgcgtcgat
     2401 gaggaaacgg gcggcatcac cgtcgagcgt tacatggtgt cctacgacgt cgggcgcgcg
     2461 gtcaatccta tgctgatcga aggccagatc cagggcggcc tcgcgcaggg catgggcggc
     2521 gcgctgttcg aggaattcct gtacgacgac cagggccagc cgctgtcggt gactttcgcc
     2581 gattacctga tgccgacctc gaaggaaatg ccggcgctcg aggtgctcgt gaccgaagat
     2641 gcgcccagcc cgctcaatcc actcggcatc aagggtgcgg gcgaaggcgg cgtgaccgcg
     2701 gccggcgcgg cgatcgcctc cgcgatcgac gacgcgatct gcatccccgg ggcgattcgc
     2761 cgcctgccgg tgacgccgca acgcctgatg cagattctga agggagcaga caaccgtgcg
     2821 tgacaagtct gacaagtccg ccaagcccat attcggcgcg tcgacgccgc gcctcgagga
     2881 cgacgcgctg gtgcgcggca aggggcgtta cctggacgac ctcgcggcgc ccggtgcgct
     2941 ggccgcctac tttgtgcgca gcccctacgc gcacgcggcc atcggcgcga tcgatgccga
     3001 cgccgcgcgc gaggtgccgg gcgtggtggc catctacacg ctcgacgagc tgcgcgcaca
     3061 cctgaccgac acccagctgg tggtcggcct gccgaccaag gcctacctgc tcgatgtgaa
     3121 ccgcccggtg ctcgcgcgcg aagaggccac ctacgtcggc gaaccggtcg cggtcatcat
     3181 cgccgacacc cgtcacgccg ccgaggacgc ggccgagctg ctggagatcg actacgatcc
     3241 gctgccggcg gtgtccgact gccgcagcgc actggcggcc gacgcgccca ccgtgcagag
     3301 caagctgccg cacaacctcg ttgccgcatt cggcttcgat tacggcgacg tcgacgacgc
     3361 tttcgccggc gccgcccatg tgttccgcga atcgctgtgg gtgcatcgcg gcggctcgca
     3421 ctcgctcgaa gggcgcggcg tgctggccga gtacagcgcc gcgtccaact gcctgacggt
     3481 atggatgtcg acccagaccc cgcataccgc acggcgcatg ctgtgcgacc tgctcggccg
     3541 ctccgacgac ggcgtgcgcg tgatcacgcc cgacgtcggc ggcggcttcg gcccgaagct
     3601 ggtgttctac tcggaagaag tggtcgtcgc gctggccgcg atgctgcttg gacgtcccgt
     3661 gaaatgggtc gaggacaggc gcgagcattt cgtcgccacc acgcaggagc gcgaccagta
     3721 ctgggacgtg gagatcgccg tcgacgccga cgcgcgcatt ctcggcgtgc gcggcgcgct
     3781 gctgcacgac cacggcgcct acaccgcgcg cggggtcaat gtcacctacg gctcggcggt
     3841 caccgtgccg ctgccgtaca acgtgccggc ctaccgcatg gatgtgagca tggcgctgac
     3901 caacaaggtg ccggtcacgc cggtgcgcgg cgccggccag ccgcaggcgg tgttcgtgat
     3961 ggagcgcctg ctcgaccgcg tcgcgcgcga gctctcgctt gacggcgccg aggtgcgccg
     4021 gcgcaacctg gtgcgtcccg aacagatgcc gtgtgagaag ccgctggtgc tgcgtggcgg
     4081 gcgcaatgtg ctgctcgaca gcggcgactt catcgccacc caggcgcagg cgctggaact
     4141 ggccggctgg aagagtttcc gcgaacgcca gcagcaggcg ctcgaagagg gccgctacat
     4201 cggcatgggc ctggccaact atgtggaagg caccgggcgc ggaccgtatg aatcggtgcg
     4261 cgtgcgcatc gccggcaacg gcaaggtgct ggtgtccagc ggcgccgccg caatggggca
     4321 gagcacgaag accatgctgg cccaggtggt ggccgaacag ctcgggcgcg acatggacaa
     4381 catcgtcgtc accaccggcg acacgcaagc cgtcgcgctc ggcttcggcg ggttcaacag
     4441 ccgccagacg gtcaccgcgg gcgcgtcggc ccacgccgcc gcactcaagc tgcgcgcacg
     4501 cgtgttcgaa gtcgccgccg acttgctgaa ggccgatccg gccgagctgg agatagccgg
     4561 cacccgcgtg ttcgcgaaga cccgccccga acaggcgctc ggactcggcc agatcgcgcg
     4621 cgccgccgcc ggcctgcccg gcttcattct tccgggcggc aacggtccgg cgctggaagt
     4681 gcttgagcac gtcatcatcg acgacatgag ctattcgaac ggctcggcgg tcgtcgaggt
     4741 cgaggtcgac gtcgagaccg gcggcgtgaa gctcaaccgg gtggtgctcg cgcacgactg
     4801 cggccgcatg gtcaatccga tgatcgtcga cggccaggtg ataggcggca tcgcgcacgg
     4861 cctcggcaat gcgctgttcg aatggatggg cttcgacgcc caggcgcagc cgatcaccac
     4921 caccttcgcc gattacctgc tgatttcggc ggcggagatg ccggcggtcg agatgatcca
     4981 ccaggaatca ccgtcgccgc tgaacgcgct cggtgtcaag ggcgtcggcg aatccggcgt
     5041 gatcccgatg ccggccgcag tcgcgtccgc gatcgaggat gccttgcgcc cgcttggcgt
     5101 gcacatcgat cgcgcgccgt tgtcgccaat ggccttgcgc gcgctgatcc gcggcgggcg
     5161 tggggaagat gtcgaacacg actggcgcgc cgactcggac cgcatccgcg ccgcgcacgc
     5221 cggagccgcg catggataag gcgctccacg aactgggcga ggaactcgcg ctcgcccacc
     5281 acatcctgat cgaccagggc gtgctggagg ccttcggcca cgccagcgcc cgcctgcccg
     5341 gcgaccccgg gcgcttcatg ctgcccgcgg ccggtgcacc cagccgcatg cgcgccgccg
     5401 acatgctggt gttcggcatg gatggcgagc cggtcgcgcc gaccgaacgc gcgttgttct
     5461 cggagcgctt catccacgcc gcgatctacc gcgcacggcc ggacgtaatg gcgatctgcc
     5521 atcaccactc gccgtcgatc atgccgttct gcgtgagcgg ggtgccgctg cgcccggtgt
     5581 cgcagaccgg cgcgtcgatg gggccgcagg tgccgttctg ggacagccgc agcgaattcg
     5641 gcgacaccaa gctgctcgtg acgaccgacg ccgaagccga ctcgctggcg cgtgcgctcg
     5701 gcccggcctg gatggtgctg atgcgccgcc atggcgccac ggtcgtcggc accagcgtca
     5761 aggagctggt gtttcgcgcg gtgcactact gccgcgacgc cgatcaccag ctgcgcgcgg
     5821 cggcgctggg cacgatcgat cccttgacac gcgcggaatg ctcgctcgcc ggccagctgc
     5881 gcgccgaacc ggttgcgcgc tgctgggaac actggaaggc cctgctgccc gcccagttga
     5941 ttgaacgact tacaggaact gcagaatgaa acccgcaccc tttacctacc accggcccgc
     6001 ttccgtcgac gaggcgctag ccatcctggc cgacgtggcc gaggaaggcg gccgcgtgat
     6061 cgccggcggc cagagcctga tcccgatgat ggcgctgcgc gtggcctatc cgccgcacct
     6121 gatcgacatc aacgaaatcg cggagcttgc ccagaccagc gtatccggca ccacgttttc
     6181 tatcggcgcg ctcacacgtc acgcgcgctt tcacgaaccg gtcgcgccgg gcccgctcgg
     6241 cccgctgatg tcggcggttg cgcatcacat cgcgcattac ccgatccgcc agcgcggcac
     6301 cttctgcggc agcctcgccc atgccgatcc ggcgtccgaa tggtgcctgg tcaccgcgac
     6361 gctggatggc gtgatcgtgg tgcgcaacaa gaacggcatg cgcgagatcc cggtcggcga
     6421 gtacttccag ggcgtgatgg cgacggcggt ggaaccggag gaaatgctgg tcgaggtgcg
     6481 gctgccgctg ttggccgacg acgaacgctt cggcttcgtc gaattcaacc gtcgcgcggg
     6541 tgacttcgcg ctcggcatga gcctggtcac gctgcgcatc gcagaaggcg tgatcgcctc
     6601 ggcgcgcgtg gggctgggcg gggtggaaga acatccgcgc cgcatcgccg aggcggaagc
     6661 cgaactggtc ggccgggcgc cgaccgacgt agtgtttcgc aaggctgccg cagccgcggc
     6721 agccgtggtc gagccgctgg aagacgcgca gaccagcgcc gattaccggc gcgacctgac
     6781 cgcggtggtg attcgccgcg cgctcgagca ggccatgcgc gatggccgct gaagactgaa
     6841 aggacaagga ccatgaccga caagaccgac attaacctgg acgtgaacgg cggatcgttt
     6901 gccgtgcgcg tggagccgcg caagaccctg gccgatacgc tgcgcgaaga ctgcggcctg
     6961 accggcaccc acgtcggctg cgagcacggc gtgtgcggcg catgcacggt gctggtcgac
     7021 ggccagccgg tgcgctcctg cctgatgttc gcggtgcagg cgcagggcca tgaagtgcgc
     7081 acggtggagt ccctcgccga cggcgacacg ctcaaccggc tgcagcaggc gttctgcgac
     7141 aaccacgcgc tgcagtgcgg cttctgcacg cccggcttcc tgatgctgat caccggcgcg
     7201 ctcgaaaaga atcccgatat gaccgacgat gaaatcgtcg aggtcctgtc ctccaacctg
     7261 tgccgctgca ccggttacat caacatcgtc aaggcggtga agcaggtgag ggacatgggc
     7321 aaagcggcgg caccggccgc cggcgcagcg caagccgcgt aaggccagcc cggcggcgca
     7381 acgcggcttg cgccgtgccg ccgattttga acacgcgaga aaggtatccg acatggaaat
     7441 gagtggaagc gagcgcatcg gcgccagccg cgaggcagtg tacgcagcat tgaacgatcc
     7501 cgagatcctg aagcaggcgc tgccgggctg ccaggagctg gagaaagtgt ccgacaccga
     7561 gttcaacgcc gtcatcgtcg tcaaggtcgg cgtgatcaag gcgaccttca agatggccgg
     7621 cacgctgatg gatctcgatc cgccgaacgg ctacacgctc aagggcgaag gcaagggcgg
     7681 ggtcgccggc ttcgccaagg gcagtgccca ggtgcgcctg gaggaagacg gcgacgccac
     7741 gatcctgcac tacacggtcg cagccgacat cggcggcaag ctcgcccagc taggcgcgcg
     7801 cctgatcaat tcgaccgcga tgaagttcgc cggcgagttc ttcggcaact tcagccgcat
     7861 cgtcgccggc gagggcgcgg caccggcgca ggaagccgcc gcctgaatct gactgacttg
     7921 ccgtacgagg aacttgtgac gaacacgata ctgacgacgc tcagcccgca gcggctgaag
     7981 cagcactacg aagaagggtt ctggcagagc gacaccatct acatgctggc gcgcaagaac
     8041 gcgcaggccc atcccgacca ctacgcgttg cgcgaccgct atcgccgcct gaccttcgca
     8101 caggcggtcg aagcggccga ccggctggcg gcccacctgg ccgctcaggg ggtgcgccgc
     8161 ggacagcggg tcgcggtatg gctgtcgagc cgcctcgaaa ccgcaatcgc gctcctggca
     8221 tgctcgcgca acggctatgt ctgctgccct tcgctgcacc gcaatcacac ggtgggcgag
     8281 atcggcgaac tgatggagcg catgcgcgcg gccgcgctgg tggccgagat cggctacggc
     8341 gccgacgccg accgccacga cgtgttcgcg ctggccgagg cgcagaagac catgcgcgcg
     8401 atctatcgcg tgccggcgct ggctccgggc gatgcacagc acctgccgtt cggccctctg
     8461 ctggccaccg gcgccggtgc ggccgacgcc acgccgttcg acaccgagcc cgataccctc
     8521 gtctacctcg cgttcacctc cggcaccacc ggcaagccca aaggtgtcat gcacagcgac
     8581 aacacgctgc tggcgaatgc gcgcgcgctc gccggcgact ggtcgctcgg ccccggctcg
     8641 gtgatctaca cgctcagtcc gctgtcgcac aacctcggct tcggcgcgat ggtcgccgcg
     8701 ttcgcggtcg gcgcggaact ggtcgtgcat gacacgcagc gcggagaaag cctggccgaa
     8761 cgccttgccg agaccggcgc gaccttcatg ttcggcgtgc cggtgcacgc gatcgacctg
     8821 ctcaaggagc tgcgcgagcg cgatgccgcg ctggtgaaga ccgtcaaggg tttccgcatc
     8881 tccggcgcgc cggttccgca ggaagtcgcg gccgagctgc tgcaatacgg ggtggtgcca
     8941 cagagcggct acggcatgac cgaagcctgt tcgcaccact acacgctgcc caccgacgat
     9001 ccgaagatga tcatcgaaac ttccggccgc agctgccccg gctatgagat ccggatcttc
     9061 acgcgcgacg acccggaaaa ggaagcgccg gtcggcgaag tcggccagat cggcgggcgc
     9121 ggcgccagcc tgatgctcgg ctactacgac aaccaggagg cgaccgagga ttcgttcaat
     9181 gcccagggtt ggttcatgac cggcgacctc ggctgggtcg atgccgaagg ctatctgcgc
     9241 atcaccggcc gcaagaagga cgtgatcatc cgcggcggcc acaacatcta cccggccaag
     9301 atcgaggcgc tggccggccg tcacgcggcc gtggaacgcg cggccgcggt gccggtggcc
     9361 gacgagcgca tgggcgagaa agtctgcctg gtgctgatgc cgcgcgccgg caagctggtc
     9421 gacccgatgg acatgctggc ccacctggac gcaaccgggc tgtcgaagtt cgacatgccc
     9481 gaatacatgg tgctggtcga cgacattccg ctcaccgcca gcggcaagat tttgaagcgc
     9541 gacctggtcg atcagatcaa ggccggcacg cttgtgccgc agccggtgcg ctgggccgga
     9601 cccaaggcat ccgccgccgt cgccgaggcg gttcaatgag tgagcaggcg caggcgctcg
     9661 cgcgcctgga agaggaaatg cggcgtccgc cgtttcacgc ggtgctggcg ccgcaggcgc
     9721 acgcggtcga tccggaaagc ggcgaggtgg taatccgcct gccgtttcat ccggacttcg
     9781 gacgcgatcc ggtccacgtc ggcttccacg gcggcgtgat cgcctcgctg atcgacctgg
     9841 ccggccacgc ggcggtcgcc gtcaaggtcg gcaagccgac tccgaccgtc gacctgcgca
     9901 tcgactacct gcgcccggcg ccgggcgtga cgctggaagc gcgcgcgcgc gtgctgcgcg
     9961 ccggccgggc aatcgcgcgc gccgatatcg acgtgctggc tgacggggtg ctggtggcgg
    10021 tcggccgcgg aaccttcagc accctgtagg cgcccggcaa aacagaacaa ggaatgaaca
    10081 tgcgtgcagt gatcatcaat gaattcggaa acatcgacac accggtgtac ggcgaaatgc
    10141 cgacgccccg tccgggaccg ggcgaggtcg tcatcagggt gcgcgccacc gcggtcaact
    10201 tcgtcgatct gctggtgatt ggcgggaagt accagttcct gcctgagcgc ccgttcacgc
    10261 cgggcaaggg accggccggc gtcgtcgccg cagtcggcga aggcgtggcc agcctgaagg
    10321 ttggcgaccg cgtgctggcg atggccgagc acggcggcta cggcgagcag gcgctggtgg
    10381 cggccaacca gtgctatccg ctgccggcgg gcatgagctt tgccgaggcg gcctcgatgt
    10441 cactcgcgta tgacaccgcc tggttcgcgc tgcgcgagcg cggtcgctac aaggaaggcg
    10501 agacggtgct ggtgctgggc gcaagcggcg gcgtcggtta cgcggcggtg cagctggcca
    10561 aggcgatggg cgcgaaggtg ctggccggcg tgtcgtcgcc gtccaaggcc gacatggtgc
    10621 gcgctgccgg cgccgacgcg ctgatcgacc tgtccgccgg gaatctgcgc gacagcctgc
    10681 gcgagcaggt gtacgcggcg accgacggca agggtgcgga cgtcgtcatc gacatgctcg
    10741 gcgacgacat ctttgacgcc gcactgcgcg cggtcgcctg gcgcgggcgc atcgtcgtgg
    10801 tcggctttgc cgccggccgc attccgacca tcaaggccaa ctacctgctg gtgaagaaca
    10861 tcgaggcttc cggcctgcag atcagcgatt accgcaagcg catgcccggt ctggtggccg
    10921 aatgcttcgc cgagatcttc gcgttctacg aagccggcaa ggtgcgtccc gccccgtaca
    10981 ccacgctgcc gctgtccgag ttccgcgacg gcctgcgcat ggtgcaggac cgcacggcca
    11041 agggccgcgt ggtcctcgtg caggacgatg aataaccgta ccgggaaccc gcgatgacga
    11101 tgcccttgcc cactcacaac cgctacccgt tttcgctgat cgaccagcgc aaggatttca
    11161 gctggcccga cggcaagcgc ctggccttct acgtctgcaa caacatcgag tacttcgcgt
    11221 tcggcgccgg cctcggctcc gagatcggca tggtgaatcc gccgccgaac caccgcagct
    11281 atgcgtggcg cgactacggc aaccgcgtcg gcctgtctcg catactcgac ctgctcgacg
    11341 agctcggcct gcccggcgcg cacaacgtca actccttcct gtacgacgcg catccggaaa
    11401 tcttcgagcg catccgcaag cgcggcgacg agatcgtcgg gcacggccgc accaatgccg
    11461 agcgccagtc gtcgatgtgg gaggcagacg aagcgcgcct gatccgcgaa gcgaccgatg
    11521 cgatcgcgaa gcacgaaggc
//
LOCUS       DATLGF010000451        12444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_12173,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000451 DATLGF010000000
VERSION     DATLGF010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 12444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12444
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12173"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VM406_13810"
     CDS             <1..431
                     /locus_tag="VM406_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HVL77085.1"
                     /translation="RDGGATAKAIIAQVRRTAEEMADASSPDLAAIRGRVEDGAQALE
                     EVVAYVVANMKSDIKGVFAGSVLYLKLAGIVLGGWQMARAALIAERKLAAGEGDIDFL
                     KAKIGTARFFADHFLSHAGAMRTAIIEGSAGVLALGEEQF"
     gene            complement(463..621)
                     /locus_tag="VM406_13815"
     CDS             complement(463..621)
                     /locus_tag="VM406_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77086.1"
                     /translation="MITSGSETAMPSDTAVPACMRGAHAEGIPHLFTIEKIEKNAGSS
                     IDKKIKAA"
     gene            620..1075
                     /locus_tag="VM406_13820"
     CDS             620..1075
                     /locus_tag="VM406_13820"
                     /inference="COORDINATES: protein motif:HMM:NF027482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4864 domain-containing protein"
                     /protein_id="HVL77087.1"
                     /translation="MKRFLLIAGLVFGLGGPCWAHAEPVEQPHEALAITAEDAAAIHQ
                     TVQAQLQALANDDAAAAFELATLEKRMLIGSPDNFLRLMQELYEPLYRHTAVIYFKPE
                     VVHGNAVQMVRLTDSSSRVWVAIFWMQQDDEHAWKIDGCHLLETMSVSI"
     gene            1388..1603
                     /locus_tag="VM406_13825"
     CDS             1388..1603
                     /locus_tag="VM406_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HVL77088.1"
                     /translation="MTRKYVDCREFPSEMNCSVAMAADSEEELIEAAVQHAVAVHHHE
                     DTPEFRQQIRQTIHEGTPPAERPAVAH"
     gene            complement(1617..2033)
                     /locus_tag="VM406_13830"
     CDS             complement(1617..2033)
                     /locus_tag="VM406_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HVL77089.1"
                     /translation="MTASHKNKTFATFLATIFGGVGLHRFYLHGSKDFWGWIHAATLA
                     LSLLLYLARPEQPLMFSFLPFVLSALAALLAALVIGLTADEQWDARHNPHSGRHSASG
                     WPLAVLLVLAFGVGAIGLTATIARMFDLLYTGGAFG"
     gene            2190..2441
                     /gene="rpsP"
                     /locus_tag="VM406_13835"
     CDS             2190..2441
                     /gene="rpsP"
                     /locus_tag="VM406_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HVL77090.1"
                     /translation="MVVIRLSRSGAKKRPFYNIVATDSRNRRDGRFIERIGFYNPVAS
                     GSDQTLRIAEDRLTHWQSVGAQLSPTVARLVKQAAAKAA"
     gene            2469..2996
                     /gene="rimM"
                     /locus_tag="VM406_13840"
     CDS             2469..2996
                     /gene="rimM"
                     /locus_tag="VM406_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078456.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HVL77091.1"
                     /translation="MTAPEDLVLVGHVTGAYGIKGWVRIRPYSADASALLHAKTWWLD
                     KPELRDVDVMQAKVHGDDVVAQVMGVADRGAAEALKGATVQVRRSHFPVLDEDEFYWV
                     DLIGLDVENLEGEPLGTVHDLMDNGAHPILRVQSGDPQEDGKRVERLIPFVDQYVKTV
                     DRAGKKITVDWGLDF"
     gene            3021..3779
                     /gene="trmD"
                     /locus_tag="VM406_13845"
     CDS             3021..3779
                     /gene="trmD"
                     /locus_tag="VM406_13845"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HVL77092.1"
                     /translation="MQFDVVTLFPEMFVAITQSGITRRAFEQRKCGLKLWNPRDFTSD
                     NYRTVDDRPYGGGPGMVMLARPLEAAIDAARARQAELGVPESRVIYLSPQGRALTHER
                     VMQLRADPGLVLLCGRYEAVDQRLLERCVDEEISLGDFVLSGGELPAMALMDAVIRQL
                     PGVLNDDVSAVEDSFVNGLLDCPHYTRPEVYEGVEVPAVLMSGNHARIEAWRREQALA
                     ATQRKRPDLIAKARAAGRLSKADEKFLESLQYQQ"
     gene            3876..4259
                     /gene="rplS"
                     /locus_tag="VM406_13850"
     CDS             3876..4259
                     /gene="rplS"
                     /locus_tag="VM406_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HVL77093.1"
                     /translation="MDLIQQLEQEEIARLGKNIPDFAPGDTVVVNVNVVEGTRKRAQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPLIASIEVKRRGDVRRAKLYYL
                     RERSGKSARIKEKLPSRRSVEQAAE"
     gene            4388..5056
                     /locus_tag="VM406_13855"
     CDS             4388..5056
                     /locus_tag="VM406_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HVL77094.1"
                     /translation="MTKLVFDPETLPVASIAGEPAVPPEHLSQDWLRRRFADPPAWVP
                     EDYEESVLRHQVRAPIPASVLMPLVKREGGLTLLLTRRTDHLSAHAGQVSLPGGRMDA
                     IDDSAIETALRETEEEVGLHRRHLEVLGTLPDYVTGTGFRVTPVVALANPPFELTADP
                     FEVAEIFEVPLAFLMDGANHQRRTAILPDGRERTFYAMPYQRYFIWGATAGMLRNLYH
                     FLRA"
     gene            5136..6089
                     /locus_tag="VM406_13860"
     CDS             5136..6089
                     /locus_tag="VM406_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HVL77095.1"
                     /translation="MTFLSILVALLIEQVKPLRADNPVYAAVRQFSVRMERSFNAGEE
                     SHGRVAWLLTVGALLVPTLILYWICAAISPFAVLVFNVVVVYLTLGFRHYSHYFTSIQ
                     LALNSGDEATARRLLAEWTKEDTAALGAREIARVAVENALVTTHRNVFGVFFWLLLPF
                     GPAGAVLYRLSEHLARSWNEPAHLRHEPFGRFATRMFYWIDWIPARLTAIAFAIVGNF
                     EDAIYAWRNFAHRWKDEAIGIILSAGGGALGLRLGNARETAVDLAPADPILEATAGLP
                     DSPPGEEATARALQSTVGLLWRALLLWMLLLLLASVAAWMG"
     gene            6337..6672
                     /locus_tag="VM406_13865"
     CDS             6337..6672
                     /locus_tag="VM406_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HVL77096.1"
                     /translation="MADSTPEYTGEFFIQGITSDGRQFRPSDWAERLCGVMAHFRPEG
                     TGGRNAHLGYSPYVRPTVLNGVKSVVVNEKLRALEPLAYHFVVNFAKDNDLQIVDACL
                     LPERPRPPG"
     gene            6772..7203
                     /locus_tag="VM406_13870"
     CDS             6772..7203
                     /locus_tag="VM406_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HVL77097.1"
                     /translation="MKHARGFTLIEIMIVVVIVGILASVAIPSYQRYVIRTKIQEPMS
                     LLADMRIRYEQYFQDNRTYVGLVDNACNAEVGSNDVPSARYFSYRCESGANTFTITAN
                     GIASEGMSGYRFTINESNARTSTLPGSAAVSCWRTQASESC"
     gene            7197..7736
                     /locus_tag="VM406_13875"
     CDS             7197..7736
                     /locus_tag="VM406_13875"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HVL77098.1"
                     /translation="MLGHPPDRGFTLVELMIVLAVLAIILGIGMPSFSLWMENNRIRN
                     AAESIISGLHLARSEAVSRNSLVQFRLVTGSAWTVGCVNAMAGCPATIQSRSIGDGSS
                     ASVTVDADGEDTFVFDGFGRMAAPVPTGGATTIAIAVDSSVLDASRSRDLRITASPGG
                     GVRLCDPNISSTGDPRRCP"
     gene            7745..8158
                     /gene="pilV"
                     /locus_tag="VM406_13880"
     CDS             7745..8158
                     /gene="pilV"
                     /locus_tag="VM406_13880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HVL77099.1"
                     /translation="MKSRSKYASHPQFGIALIEVLIAVLIFSIGLLGLVGLQASMVRA
                     SADARYRAQASFVAQQRLGQIWVDQGNMSAYVEAEPGTDIAAESGLPGGRRITLRGDA
                     SCGGDLSCFTVRVIWQQPGAVETHNVTTVTRIAGG"
     gene            8161..9192
                     /locus_tag="VM406_13885"
     CDS             8161..9192
                     /locus_tag="VM406_13885"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HVL77100.1"
                     /translation="MRAMQARRDAGFGLAEVMVAVAIGLITTLVITQVMINFDGRKRE
                     TTGAADAQLNGSIALYSIQRRLQSAGYGLPVYSAQNQPLACDPVAFDNDDDPATADVT
                     VDLFPVRIVDGLNGTSDRITLRWGPTQMGGIPIGVTSVAGNAVGIDNNMMCAVGDVAL
                     AMNGASCQMSRVRELDGTTQVVLADAGGIALGSSFTCLGNWSETTYQVAGSTLLENGV
                     PVAAGVVSLQAQYGVSDAPSSNQVTAWVDASGDWAIPAPADRNRIRAVRVAVVARAEQ
                     LEAANVTQACSSLTEAGPTGLCAWEGTDDSPAPDLDLSATPSWQRYRYRVFETIVPLR
                     NMIWSSDTL"
     gene            9189..9812
                     /locus_tag="VM406_13890"
     CDS             9189..9812
                     /locus_tag="VM406_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77101.1"
                     /translation="MKTYAFTMRRTTRGRERGAVLFLALIALITLMLAAAALTRSVDT
                     NTVVAGNLAFRHAATAAADAGVERAIAWLEAENQANAARDAFEDADHGFNTDQPALGY
                     YSAIDPARNLFAEATWANGSSACAAADGVGNTVCYIVERMCRNPGEVLSETHCLFTDA
                     EIDTASKRVRDAGSAGAKTPGRIPLNRITVRVVGPRNTVSYVQAFVY"
     gene            9821..>12444
                     /locus_tag="VM406_13895"
     CDS             9821..>12444
                     /locus_tag="VM406_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77102.1"
                     /translation="MRIRESVLLSVRLALVAAALHAPLPALAGPVQLATSPLTNATTS
                     NVLPNLMFILDNSGSMAWDFTPDYISETSGTPKATDKQCRDSGDDDNGVAYSGAMNGN
                     TRVLDLCAVGDVPFMTSSMNRQYYNPAIRYRPGVNFRGESLASQTDPTKVQTDPYGKQ
                     RTNQLLQSKTLVDLTTEYPDRVWCTKNDPTDAELNNPSVCRRNSGYLYPDATYRYGRR
                     KDTGGPDQRVTQTMLNGVLKVQGAPYYYSVVPSEYCDAADLRNCVAASAPTGNYTFPA
                     HSRWCTDAMLTNCQAVRTASYTFPRYIGSPGATATAAVGTLRVRESRNLNVTSIRVNN
                     VEILGSTVAWGNSNSNSGFAAAIAAQINTYTSNPNYTATVSGDTITLSSTVAAGAAGN
                     GPISLSGTLSNYTLSSGGMTGGKDAINALAPYTFARTNIVPSVNSYPKAGGRTDCAGT
                     TCTYAEEITNFANWYAYYRTRMQMMKTSATLAFKGIGDNFRVGFITISSQTTTGHYLP
                     IDRFSSGAAGANSQKERWYSTLLNINPSGGTPLRSALSIVGRIYAGQKPVGNSDPVQY
                     SCQQNFALLTSDGYWNTDTNSVVRGLDGNPVGNLDSDPSTRPVYEGPTATANTLADVA
                     KYFYDTDLRDPKWGNCTGALGEGIDVCENNVFTTSTDLNTKQHMTTFTLGLGIDGTLN
                     YTDDYMTATSGDFYRLKTGTGSPVVNWPIPVADTETAVDDLWHAAVNGGGIYFNAQDP
                     TQLAKGLEGALASIGAKIGAGAAAATSTLNPVPGDNFAYVASYTTQKWHGNLEARSIN
                     LLTGVVSESAAWCVESVIAESCSGTVVSEDTGSSRAFYCVRQNVAEESCEGQFDGSSC
                     KVQMATACTGTMSAKVSAS"
BASE COUNT         2319 a         3911 c         4011 g         2203 t
ORIGIN      
        1 tgcgggacgg tggtgcgacg gcgaaagcca tcatcgccca ggtgcgccgt acggcggaag
       61 agatggcgga tgcgtcatcg cccgacctgg ccgcgatccg cgggcgcgtc gaagacggcg
      121 cacaggcact cgaggaagtg gtcgcttacg tcgtggcgaa catgaagtcc gacatcaagg
      181 gcgtattcgc cggcagcgtt ctctacctga agctggcggg gatcgtgctg ggcggctggc
      241 agatggcgcg cgccgccctg atcgcggaac gcaagcttgc cgccggcgaa ggggatatcg
      301 acttcttgaa ggccaagatc ggcaccgccc gtttcttcgc tgaccacttt ctttcgcatg
      361 ctggcgcaat gcgcacggca atcatcgagg gcagtgcggg cgtgctcgcg ctcggggaag
      421 aacaattctg acccgcttca tcccctccct ataaaggcag cgtcatgctg cctttatttt
      481 tttgtctatt gaactaccgg cattcttttc aatcttctct atagtgaata aatgagggat
      541 gccttccgca tgcgcgcctc gcatgcaagc cggcacggcg gtatcggagg gcattgcagt
      601 ttctgatccg gaggtgatca tgaaacgttt tttgttgatt gccggcctgg tattcgggct
      661 aggcggaccg tgctgggccc acgcagagcc tgttgaacaa ccgcatgaag ccctcgcgat
      721 cactgcagag gatgcggctg caatccatca aacggtgcag gcgcagttgc aggcgctggc
      781 caatgacgac gcggcggcgg cgttcgaact cgccacgctg gagaagcgga tgctgatcgg
      841 atcccccgac aactttctgc gcttgatgca ggaactgtat gagccgctgt atcggcatac
      901 ggccgtcatc tacttcaagc ccgaagtggt gcacgggaat gcggtgcaga tggtgcggct
      961 caccgacagc agcagccgtg tgtgggttgc gatcttctgg atgcagcagg acgatgagca
     1021 cgcctggaag atcgacggct gccacctgct cgagacgatg agcgtctcga tctgagggtt
     1081 cggttccggc gccctgcaca tcggtgagtc gtcggggtga acgttgtgaa gctgcttgcg
     1141 gcggccgcgg ccttttcgtt gttcaattcc gaagtgatcc cgtcaggcag acccatgctg
     1201 gcgccgttct tgtccggcct ccggaagccg acaattcgat tcacatcaag tttctggcgg
     1261 acttccggat caagcagccc tgccgggatt ccggccgctt ccacgcgcgc tataacgaaa
     1321 ttgcgcggca tgccagccgc gccgcaccgg tcgagccggg ggcgactgaa gccgaggaga
     1381 gagcgccatg acccgcaaat acgtggattg ccgggagttt cccagtgaaa tgaactgttc
     1441 ggtagcgatg gcggcggatt ctgaagagga gttgatcgag gccgcagtgc agcatgcggt
     1501 ggccgtgcat caccacgagg atacgccgga gttccgtcag cagatccggc agacgatcca
     1561 tgaaggtacg ccgccggccg aacgtccggc cgtggcccat tgagacgtcc gccgcttcag
     1621 ccgaatgcgc cgccggtata gagcaggtcg aacatccgtg caatagtggc ggtcagtccg
     1681 atcgcaccga cgccgaacgc cagcaccagg aggaccgcca gcggccaacc tgaggccgag
     1741 tgacggccgc tgtgcgggtt gtggcgggca tcccattgct cgtcggcggt caagccgatc
     1801 accagcgccg ccagcagtgc cgccagcgcc gacaggacga agggtagaaa ggagaacatg
     1861 agcggctgct cgggccgggc caggtagagc agcagcgaga gtgccagcgt ggccgcgtgg
     1921 atccaccccc agaagtcttt gcttccgtgc aagtaaaagc gatgcaggcc gacgccgccg
     1981 aagatggttg cgaggaaggt ggcgaacgtc ttgttcttgt ggctggcggt catggcgtcg
     2041 tagaaagctg gggcgaccgt agtgtgcccc agcaacaagc caggcacaac ccgggtgggc
     2101 ggcaggggga ggccagcctt gtcgtgattc caaagaacgc gctataattc ccggcttttt
     2161 ccgccttcga acttttttgg aagcacatca tggtcgttat ccgtctgtcc cgctccggcg
     2221 ccaagaagcg cccgttctac aacatcgtcg ccaccgactc gcgcaatcgc cgcgacggtc
     2281 gtttcatcga gcgcatcggc ttctacaacc ccgtcgcttc gggcagtgac cagaccctgc
     2341 gcatcgctga ggaccgcctg acccactggc agtctgtcgg cgcccagctg tcgcccaccg
     2401 ttgcccgcct ggtcaagcag gcagctgcca aggcggcctg agtttagttt cccgaaaacg
     2461 ctgcggggat gactgcccct gaggatctgg tgctggtcgg gcacgttacc ggggcctacg
     2521 gcatcaaggg ttgggtgcga atccgcccgt attccgccga tgcgagcgcc ttgctgcatg
     2581 cgaagacctg gtggctggac aagcccgagc tgcgcgacgt cgatgtcatg caggccaagg
     2641 ttcatggcga cgacgttgtt gcccaggtga tgggcgtggc ggaccgcggc gccgccgagg
     2701 cgctcaaggg tgccaccgtg caggtccggc gcagccattt tccggtcctc gacgaagacg
     2761 agttctattg ggtcgacctg atcggccttg atgtggaaaa cctcgaaggc gaacccctcg
     2821 ggacggtaca cgacctgatg gacaacggcg cccacccgat cctgcgcgtg cagtcgggcg
     2881 acccgcagga ggacggcaag cgggtcgagc gcctgatccc gttcgtcgac cagtatgtga
     2941 agactgtcga ccgggccggc aagaaaatta cggtcgactg gggcctggat ttctaggcgc
     3001 ttgaaccagg aagagtgcag atgcaattcg acgtcgtcac gctctttccc gagatgttcg
     3061 tcgcgatcac gcagtcgggt atcacccgcc gtgcgttcga gcagcgcaag tgcggattga
     3121 agctgtggaa tccgcgcgat ttcaccagcg acaactatcg caccgtggac gaccgaccgt
     3181 atggtggcgg cccggggatg gtgatgctgg ccaggccgct ggaggcggcg atcgatgccg
     3241 ccagggcgcg ccaggcggag ctgggtgtgc cggagtcgcg tgtgatctac ttgtcgcccc
     3301 agggccgtgc gctgacgcac gagcgcgtga tgcagttgcg cgcggatccg ggcctggtgc
     3361 tgctgtgcgg ccgctacgag gcggtcgacc agcgtctgct ggagcgctgc gtggacgagg
     3421 agatcagcct cggcgatttc gtgctctccg gcggcgaact gccggcgatg gcgctgatgg
     3481 acgcggtgat ccgccagttg ccgggggtgc tgaacgatga tgtatccgcc gttgaagaca
     3541 gctttgtcaa cggcttgctc gattgtccgc attacacgcg gccggaagtc tacgaaggcg
     3601 tcgaggtgcc ggccgtgctg atgagcggga atcacgccag gatcgaggca tggcggcgcg
     3661 agcaggcctt ggcggccacg cagaggaagc gtcccgacct gatcgcgaag gcgcgtgcgg
     3721 cgggaaggct gtcgaaggcc gatgaaaagt tcttggaaag tttgcagtac cagcagtagg
     3781 aaagggtagc gatacccgtt gttcaacccc atcctctgcc gggcatgaag cgcaaggctt
     3841 ccactgtgcc ggcacgatgg tctaaggagt gaaaaatgga tctgatccag caactcgagc
     3901 aggaagaaat cgcccgcctg ggcaagaaca tccccgactt cgcccccggc gataccgtcg
     3961 tggtgaacgt gaacgtggtc gaaggcacgc gcaagcgcgc ccaggcttac gaaggcgtgg
     4021 tgatcgcgcg ccgcaaccgc ggtctgaact caaacttcat cgtccgcaag atctcgtcgg
     4081 gcgaaggcgt cgagcgtacc ttccagctct attcgccgct gatcgcgtcg atcgaagtca
     4141 agcgtcgcgg tgacgtgcgt cgcgccaagc tgtactacct gcgcgagcgt tccggcaagt
     4201 cggcacgtat caaggagaag ctgccgagcc gccgcagcgt cgagcaggca gccgagtaat
     4261 tccggcgcgg cacaggaaaa aggcaccctc gcggtgcctt ttttcatggg cgggccgacc
     4321 aggccagagg gcgatccgcg ctagaatcag cagctcgccg cgcgcatcgt tctccgcagg
     4381 accgcccttg accaaacttg tcttcgaccc cgaaaccctt cccgtcgcca gcatcgccgg
     4441 tgagccggcg gtaccgcccg agcatctgag ccaggactgg ctgcgcaggc gctttgcgga
     4501 tcctccggcg tgggtgcccg aggattacga ggaaagcgta ttgcgccacc aggtgcgcgc
     4561 gccgataccg gcttcggtgc tgatgccgct ggtgaagcgc gagggcgggt tgacgctgct
     4621 gctcacgcgg cgcacagacc atctcagcgc ccatgcgggg caggtgagtc ttcccggcgg
     4681 gcgcatggac gcaatcgacg actcggcgat tgaaacggcg ctgcgcgaga ccgaggagga
     4741 agtcggcctg catcggcggc atctcgaggt gctgggcacg ctgcccgatt acgtgacggg
     4801 gaccggattt cgcgtcactc ccgtggtcgc gctcgccaac ccgccgttcg agctcacggc
     4861 cgatcccttc gaggtcgcgg aaatcttcga ggtgccgctc gcattcctga tggacggcgc
     4921 caatcatcag cgccgtaccg cgatcctgcc ggacgggcgc gagcgtacct tctacgcgat
     4981 gccgtaccag cgctatttca tctggggagc aaccgccggc atgctgcgca acctgtacca
     5041 ctttttgcgc gcatgaacgg cttgcgacag ccggcggttt gattccgcac atccgctgag
     5101 ctatcgtagc ggctcgtcca cacgcaatct tcccgatgac gtttctctcg atcctggtcg
     5161 cgctgctcat cgagcaggtc aagccgcttc gcgcggacaa tcccgtctat gcagccgtgc
     5221 gccagttctc ggtgcgcatg gagcgctcgt tcaacgccgg ggaggaaagc cacggccgcg
     5281 ttgcatggct gctgacagtg ggcgcgctgc tcgtgccgac gctgatcctg tactggatct
     5341 gtgcggcgat atcgccgttt gcggtgctgg tcttcaatgt cgtggttgtg tacctgacgc
     5401 tcggcttccg tcattacagc cactacttca cctcgatcca gcttgccctg aacagcggcg
     5461 atgaagcgac ggcacgccgg ctgctggccg aatggacgaa ggaggatacc gccgccctgg
     5521 gcgcgcgcga gatcgcccgc gtggcggtgg agaatgcatt ggtgacaaca caccggaatg
     5581 tgttcggcgt gttcttctgg ctgctgctcc cctttgggcc ggccggcgcg gtgctgtacc
     5641 ggctttccga acatctcgca cgttcgtgga atgagccggc gcacctgcgc catgagccgt
     5701 tcgggcggtt tgccacgcgc atgttctact ggatcgactg gattccggcc cgcttgaccg
     5761 caattgcctt cgccatcgtc ggcaacttcg aggacgccat ctatgcgtgg cgcaattttg
     5821 cgcatcgctg gaaggacgag gccatcggca tcattctctc cgccggcggc ggtgcgctcg
     5881 ggctgaggct gggcaatgcc cgggagacgg cggtggacct tgcgccggcc gacccgatcc
     5941 tcgaagcgac cgccggcctg cccgacagcc ctcccggcga ggaagccacg gcgcgcgcat
     6001 tgcagagcac cgtcggcctg ctgtggcgcg ccctcctgct gtggatgctg ctcctgctgt
     6061 tggcctcggt ggctgcctgg atggggtgag cttgcattcc ctttcatgat gtgaaacgga
     6121 atttcggggc aagtcttcta taagatataa tatgcactac ttgcctgcta catcgtccag
     6181 gcaagacggc gctcctttgc cggccatacc ggccggcccc atggactgcg gcaacggcac
     6241 ggtccgtgct tgctgcatgg gtattccgtg cgtgcacccg atcggtgcgc gcatgcagga
     6301 gcacagagcg caacagaaaa caggcaagcc ttatcgatgg ccgattccac cccagagtac
     6361 accggagaat tttttatcca gggcatcacc agcgacgggc gccagttccg gcccagcgac
     6421 tgggcggagc gtctgtgtgg cgtgatggcg catttcaggc cggaaggcac gggcggtcgc
     6481 aatgcgcacc tcggctactc cccgtatgtc cggcccaccg ttctgaacgg cgtgaaatcg
     6541 gtggtcgtga atgagaagct gcgcgcgctc gagccgctgg cctatcactt cgtggtgaac
     6601 ttcgccaagg acaacgacct gcagatcgtc gacgcctgcc tgctgccgga acgcccccgt
     6661 cctcctggct gagcaaacct ccgctcgtcc tgaaaagccg accgaatatc ggctgcccgt
     6721 tcgatcccgg gaactttcat ctatctttcc tccttgcaaa aggggaaagc catgaaacac
     6781 gcccgagggt ttaccctcat cgaaatcatg atcgtcgtag tcatcgtcgg gattctcgca
     6841 tcggttgcga ttccgtcgta ccagcgctac gtcatccgta ccaagatcca ggaaccgatg
     6901 agcctgctgg cggacatgcg catccgctac gagcagtatt tccaggacaa ccggacctat
     6961 gtcggactgg tcgacaacgc gtgcaatgcg gaggtcggca gcaacgacgt gccgagcgcc
     7021 cggtacttca gctaccgctg cgagagcggc gcaaacacct tcacgattac ggcgaacggc
     7081 atcgccagcg aaggcatgag cggataccgc ttcacgatca atgaaagcaa tgcccgcacc
     7141 tcaaccctgc ccggaagcgc ggccgtgtcg tgctggcgta cccaggcgtc cgaatcatgc
     7201 tagggcaccc gccagaccgg ggcttcacgc tggtcgaact gatgatcgtg ctggccgtcc
     7261 tggccatcat cctcgggatt ggcatgccga gcttttcgct gtggatggag aacaaccgca
     7321 tcaggaacgc cgccgagtcg atcatctccg gcttgcacct ggcgcgttcc gaagcggtat
     7381 cgcgcaacag cctcgtccag tttcgccttg tcaccggctc cgcctggacc gtcggctgcg
     7441 tcaacgcgat ggcgggctgc ccggccacga tccagagccg cagcatcggt gacggctcgt
     7501 ccgcctcggt cacggtggat gccgacggcg aggacacctt cgtattcgac ggctttggac
     7561 gcatggcggc gccggtgccg acgggcgggg cgaccacgat cgccatcgcc gtcgactcga
     7621 gcgtgctgga cgcttcgagg agccgtgact tgcgcatcac cgcgagcccc ggcggaggcg
     7681 tgcgactgtg cgatccaaac atcagttcaa ccggtgatcc gcgccgctgc ccttgagccc
     7741 agccatgaaa agccgttcca agtacgcatc ccatccccag ttcggcatcg cgctgatcga
     7801 agtactgatc gcggtcctca ttttctcgat cggcctgctc gggctggtgg gcttgcaggc
     7861 ctccatggtg cgcgcatcgg cggacgcccg gtatcgtgcc caggcgagct tcgtcgcgca
     7921 gcagcgcctg ggccagatct gggtcgacca ggggaacatg tccgcatacg tggaggctga
     7981 gccgggtacc gacattgccg cggagtcggg gctgcccgga ggtcggcgta tcacgctgcg
     8041 cggagacgca agctgcggcg gcgacctttc gtgttttacg gtgcgtgtca tctggcagca
     8101 gccgggggcg gtcgagacgc acaacgtcac cacagtgacg cggatagcgg gaggatgaag
     8161 atgcgagcga tgcaggcacg gcgtgatgcc ggtttcggcc tggcggaggt gatggttgcc
     8221 gtcgccatcg gcctgatcac gacgctcgtg atcacgcagg tcatgatcaa cttcgacggc
     8281 cgcaagcgcg agacgaccgg cgctgcggac gcgcagctca acggcagcat tgccctctac
     8341 agcatccagc gccggctgca aagcgcgggc tacggcttgc cggtttacag cgctcagaac
     8401 cagccgctgg cctgtgatcc ggtcgccttc gacaacgacg acgacccggc gacggcagac
     8461 gtaaccgtgg atctgttccc ggtacggatc gtcgatggac tcaacggcac cagcgatcga
     8521 atcacgctgc gttgggggcc gacgcagatg ggcgggatcc cgatcggcgt cacgagtgtc
     8581 gcgggcaatg ccgtcggtat cgataacaac atgatgtgcg cggtgggtga cgttgcgctg
     8641 gccatgaatg gcgcgagctg ccagatgagt cgggtccggg agctggacgg aaccacgcag
     8701 gtcgtgctcg cagacgccgg cgggattgcg ctcgggtcga gcttcacctg cctgggtaac
     8761 tggagcgaga ccacctatca ggtcgccggc tccacgctgc tcgagaacgg cgtcccggtc
     8821 gccgccggcg ttgtcagcct gcaggcgcag tacggcgtca gcgacgcacc ctcgagcaac
     8881 caggtgacgg cgtgggttga cgcatcgggc gattgggcca ttcccgcgcc tgccgaccgc
     8941 aaccgcatac gcgcagtgcg tgtggccgtg gtcgcgcggg ccgagcagct cgaggcggca
     9001 aacgtcacgc aggcatgcag ctcgttgacc gaggccggcc caaccggact gtgcgcctgg
     9061 gaaggcacgg acgacagccc ggcgccggat ctcgacctga gcgcaacgcc gagctggcag
     9121 cgataccgat accgggtttt cgagacgatc gtcccgctgc gaaacatgat ctggtccagc
     9181 gacaccttat gaaaacgtac gccttcacga tgcgccgcac gacgcgaggc cgcgaacgcg
     9241 gcgcggtgct gtttcttgcg ctgatagcgc tcatcactct catgcttgcg gctgcggcct
     9301 tgacccgctc ggtcgacacc aacaccgtcg tcgccggcaa cctggcgttt cgtcatgcgg
     9361 cgaccgccgc cgccgacgcc ggcgtcgagc gagcgattgc atggctggag gccgaaaacc
     9421 aggctaatgc cgcgcgcgac gcattcgagg atgcggatca cggtttcaac accgaccagc
     9481 cggcgctggg ctactactcg gcgatcgacc ccgcccgcaa cctgtttgcc gaggccacct
     9541 gggcaaacgg atcgagcgcc tgcgcagcgg ctgacggcgt cggcaatacc gtctgctaca
     9601 tcgtggaacg gatgtgccgc aatccgggcg aggtgctgtc ggaaacccat tgcctgttta
     9661 ccgacgccga aatcgacacc gcttcgaagc gcgtcaggga tgccggcagc gcgggcgcaa
     9721 aaacgccggg gcggattcca ctcaaccgga tcacggtgcg cgtcgtcgga ccgcgcaata
     9781 ccgtgagcta tgtacaggct ttcgtctatt aggggacatc atgcggattc gtgaatcagt
     9841 gctgctgtct gttcggctcg cgctcgtcgc ggccgcattg cacgcaccct tgcctgcgct
     9901 ggcaggtccg gtacagctcg ccacctcgcc gctgaccaat gccacaacga gcaacgtgct
     9961 gccaaacctg atgttcatcc tcgacaattc gggaagtatg gcgtgggact tcacgccgga
    10021 ctacatcagc gagaccagcg ggaccccgaa ggcgacggac aagcagtgcc gcgatagcgg
    10081 tgatgacgac aatggcgtgg cctacagcgg cgcaatgaac ggcaataccc gcgtactcga
    10141 tctttgcgcg gtgggtgacg tgccgttcat gacaagcagc atgaaccggc agtactacaa
    10201 ccccgccatc cgatatcgac cgggagtgaa tttccggggc gagtctctgg cctcccagac
    10261 cgacccgacc aaggtacaga cggaccccta cggcaagcag cgcaccaatc agctgctgca
    10321 gtccaagacg ctggtcgacc tgacgaccga gtatccggat cgggtctggt gcacgaagaa
    10381 tgatcccact gatgccgagc tgaacaaccc ctcggtctgc cgccgcaaca gcggctatct
    10441 gtatccggat gcgacgtacc gctatggccg ccgcaaggac acaggcgggc ctgaccagcg
    10501 tgtgacccag acgatgctga acggggtgct gaaggtgcag ggggcaccct attactactc
    10561 ggtcgtgccg tccgagtact gcgacgcggc cgatctcaga aactgcgtgg ctgcatcggc
    10621 gccgaccggg aactacacct ttccggctca ttcgcgctgg tgcaccgatg cgatgctgac
    10681 gaactgccag gcagtgcgca ccgcttcgta tacgttcccg cgctacatcg gttcgccggg
    10741 cgccaccgct accgccgccg ttggcacctt gcgcgtgcgg gagtcccgca atctgaatgt
    10801 caccagcatc cgggtcaaca atgtcgaaat ccttggaagt acggtggcgt ggggcaacag
    10861 caacagcaac agcggcttcg ccgccgcgat cgccgcacag atcaacacct acacttcgaa
    10921 tcccaactat acggcgacgg tatccggaga caccattacg ctgagttcca ccgtcgccgc
    10981 cggcgcggcc ggcaatgggc ccatcagcct cagcggtacg ctctcgaact acacgttgtc
    11041 gagcggggga atgacgggcg gcaaggatgc gatcaacgca ctggctccct atacatttgc
    11101 gcgaaccaat atcgttccat cggttaacag ctacccgaag gccggtgggc ggaccgactg
    11161 cgccggaacc acatgtacct atgcggagga aatcaccaac ttcgcgaact ggtacgcgta
    11221 ctaccgcacc cgcatgcaga tgatgaagac gtcggccacg ctggcgttca agggcatcgg
    11281 cgacaatttc cgggtcgggt tcatcaccat ctccagccag accacgaccg gccactacct
    11341 cccgatcgat cgcttctcct cgggggcagc cggggcgaat tcccagaagg agcgctggta
    11401 cagcacactc ctcaacatca atccatccgg cggcacgccg ttgcgttcgg cgttgagcat
    11461 cgtgggccgc atttacgccg gccagaagcc ggtaggcaac tccgatccgg tgcagtactc
    11521 gtgccagcag aacttcgcgc tcctgacgtc agacggctac tggaacaccg atacgaactc
    11581 ggtggtccgc gggcttgacg gcaatcccgt ggggaacctg gacagcgatc cttcgacgcg
    11641 tccggtctac gaaggtccga ccgcgacggc caacacgctg gcggacgtcg caaagtactt
    11701 ctatgacacg gacctgcgcg accccaaatg gggcaactgc accggtgcgc tcggggaggg
    11761 aatcgatgtc tgcgagaaca acgtgttcac caccagcacg gacctgaaca cgaagcagca
    11821 catgacgacg tttacgcttg gtctgggcat cgacggcacg ctgaactaca ccgacgacta
    11881 catgaccgcg acgagcggtg acttctaccg tctgaaaacc gggaccggct cacccgtcgt
    11941 gaactggccg attcccgtcg cagataccga aactgcggta gacgatctgt ggcacgcggc
    12001 ggtcaacggc ggcggcatct atttcaacgc ccaggacccg acgcagctgg ccaaggggct
    12061 ggagggtgcg ctggcttcga tcggcgccaa gatcggcgcg ggtgcggcgg ccgccacgag
    12121 caccttgaac ccggtcccgg gcgacaactt tgcctacgtc gcgagctata cgacgcagaa
    12181 atggcatggc aatctcgaag cacgttcaat caacctgctg accggcgtgg tcagcgaatc
    12241 ggctgcgtgg tgcgtcgaaa gcgtcatcgc cgaatcctgc tcgggcacgg tggtatcgga
    12301 ggacaccggc agcagccgcg ccttttactg cgtgcggcag aacgtggccg aggagagctg
    12361 cgaaggccaa ttcgacggat caagctgcaa ggtgcagatg gccaccgcat gcacaggcac
    12421 gatgagcgcc aaggtgtccg cgtc
//
LOCUS       DATLGF010000452         2241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_154563, whole genome shotgun sequence.
ACCESSION   DATLGF010000452 DATLGF010000000
VERSION     DATLGF010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154563"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VM406_13900"
     CDS             complement(<1..195)
                     /locus_tag="VM406_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HVL77103.1"
                     /translation="MHIHILGICGTFMGGLAVLAKEAGHRVTGCDANVYPPMSTQLQA
                     QGIELIEGFGPEQLELRPDLY"
     gene            337..942
                     /locus_tag="VM406_13905"
     CDS             337..942
                     /locus_tag="VM406_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77104.1"
                     /translation="MSSSSIEQLRAEIAAAAARMIAEDGADYGTAKKKAARQILGNTR
                     VRGDYMPDNAQVEDEVRAYNEIFFGDTQPARLLHLRQLALRLMNDLAEFSPFLTGAVL
                     NGTAGEHSDIHLQLFVESAKDVEIFLLNRNVDFTVSEAPPSASPATPMETASFMWHNE
                     GVHLALYPLDALRLIPRNTADGRPVRMDANGLRALLEEEQS"
     gene            939..1460
                     /locus_tag="VM406_13910"
     CDS             939..1460
                     /locus_tag="VM406_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HVL77105.1"
                     /translation="MKRSSLLFAAVAVLFAVLGAAIAWWRLAPGPAEPAAVDVLFAQT
                     LPDPHGQAQPLGQWRNSTVVLNFWATWCAPCVDEMPELAELHEELSPQGTHVLGVGID
                     THQNIAQFAEKLGIEYPLFVGGAGATELARRFGNQSGGLPFTVLIDATGEVRKTYLGR
                     LKMDELRRDLANL"
     gene            1568..2005
                     /gene="aroQ"
                     /locus_tag="VM406_13915"
     CDS             1568..2005
                     /gene="aroQ"
                     /locus_tag="VM406_13915"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HVL77106.1"
                     /translation="MAKHLLLLNGPNLNLLGTREPEVYGATTLAEVERAAEVQAQAAG
                     ARLAAFQSNHEGALIDRIHAARQEGIDAIVINPGGLTHTSVALRDALAGVAIPFVEVH
                     ISNIHKRESFRHHSYLSEIAAGVICGLGVHGYAAAIEFALKKL"
     gene            2080..>2241
                     /locus_tag="VM406_13920"
     CDS             2080..>2241
                     /locus_tag="VM406_13920"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883395.1"
                     /note="composes the biotin carboxyl carrier protein
                     subunit of the acetyl-CoA carboxylase complex, the enzyme
                     that catalyzes the carboxylation of acetyl-CoA to
                     malonyl-CoA, which in turn controls the rate of fatty acid
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HVL77107.1"
                     /translation="MDLRKLKTLIDLVAESDIAELEVTEGESKVRIVKSSGAPQNQLV
                     MMPQPQAAHT"
BASE COUNT          429 a          731 c          685 g          396 t
ORIGIN      
        1 gtacaggtcc ggcctcagct cgagctgctc gggcccgaag ccttcgatca gctcgatgcc
       61 ctgagcctgc agctgagtgc tcatcggagg atagacgttg gcgtcgcagc ctgtgacccg
      121 gtggccggct tccttggcca gcacggccag gccgcccatg aaggtgccgc aaatgccgag
      181 gatatgtata tgcatgatgc aaggagtgat aacgggatgg caacgcgggc cgcagcttgc
      241 ggtcaaaccg cgtgcgcgct gcgcgagcgc gatggcgaac cggggatttt acccgacggc
      301 ctcgcctgcc gcgcggtatc atcccgaccc atgatcatgt cttcctcaag tatcgagcaa
      361 ttgcgcgcgg agatcgcagc cgccgccgcg cgcatgattg ccgaagacgg tgccgactac
      421 ggcaccgcga agaaaaaggc ggcgcgccag atcctcggca acacgcgggt gcgcggcgat
      481 tacatgcccg acaatgccca ggtcgaggac gaagtgcgcg cctacaacga aatcttcttc
      541 ggcgacaccc agcccgcccg tctcttgcat cttcgccaac tcgcgctgcg gctgatgaat
      601 gacctcgccg agttctcccc gttcctgacc ggggcggtac tgaacggcac cgccggcgag
      661 cattcggaca tccacctgca gctgttcgtc gaaagcgcga aggacgtgga gattttcctg
      721 ctcaaccgca acgtcgactt caccgtgagc gaggcgccgc cgtcggcctc cccggccacg
      781 ccgatggaga ccgccagctt catgtggcat aacgaaggcg tgcacctggc cctgtatccg
      841 ctcgacgcgc tgcggctgat accgcgcaac accgccgacg gacgcccggt gcgcatggac
      901 gcgaacggcc tgcgcgcact gcttgaggag gaacagtcat gaaacgctcc tcgctcctgt
      961 tcgcggcggt ggccgtgctg tttgccgttc tcggggcagc gatcgcctgg tggcggcttg
     1021 cacccgggcc ggccgaaccg gcagcggtcg acgtgctgtt cgcccagacc ctgcccgatc
     1081 cgcatggtca ggcccagccg ctgggtcagt ggcgcaattc gactgtggtg ctgaatttct
     1141 gggcgacctg gtgcgcgccc tgcgtcgacg aaatgccgga actggccgaa ctgcatgagg
     1201 aactctcgcc ccaaggcaca catgtgctcg gggtcggcat cgatacgcac cagaacattg
     1261 cccagttcgc cgaaaagctg ggcattgaat atccgctgtt cgtcggcggc gcgggggcga
     1321 ccgaactggc gcgccgcttc ggaaatcaat ccggcggact gccgtttacg gtcctcatcg
     1381 acgccaccgg agaggtccgc aagacctacc tcgggcgact caagatggac gaattgcgcc
     1441 gcgatctcgc caatttatag accgatcgtg cgtttttcgg tttggttgcc gggcacgctt
     1501 tgttcttgcc agccggcatg atttaccggc aaaatgcgcc atcctcggga aacccagccg
     1561 aaactccatg gcaaaacacc tgctcctgct caacggtcca aacctcaacc tgctgggcac
     1621 gcgggagccg gaagtgtatg gagcgacaac gctggccgag gtcgagcgcg cagccgaggt
     1681 gcaggcgcag gcggcgggag cccgccttgc cgccttccag agcaaccacg aaggtgcgct
     1741 gatcgaccgc attcacgcgg cgcgacagga ggggatcgac gcgatcgtca tcaacccggg
     1801 tggcctgacc cacaccagtg tcgccttgcg cgatgcgctg gcgggggtgg cgatcccttt
     1861 cgtcgaagtt cacatctcca atatccacaa gcgcgaaagc tttcggcacc actcgtacct
     1921 gtcggagatc gccgccggag tgatttgcgg gctgggtgtg cacggttatg ccgccgccat
     1981 cgagttcgcg ctcaaaaagc tctaactttc cctatcttta cgccatcggc atataatcag
     2041 ccgtttgacg cgaccaatta catattccaa ggggttctca tggatctaag aaagctcaaa
     2101 acgctcatcg acctggttgc cgagtccgac atcgcggaac tcgaagtcac cgaaggtgaa
     2161 agcaaggttc gcatcgtcaa gtcgtccggc gccccgcaaa accagctggt catgatgccg
     2221 cagccccagg ccgcccatac c
//
LOCUS       DATLGF010000453         5555 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_184754, whole genome shotgun sequence.
ACCESSION   DATLGF010000453 DATLGF010000000
VERSION     DATLGF010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184754"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1494)
                     /locus_tag="VM406_13925"
     CDS             complement(<1..1494)
                     /locus_tag="VM406_13925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HVL77108.1"
                     /translation="MSLTSLIFSTGARRRPEGWLGQALKLYAVLTAGYTVYGAAFSSW
                     DNLARIIIFLCLMLVLVFLLIGPRPSSSTTKPALIDYALSGLSLASLVFFFSEMENIA
                     VRISLFDELSGSYFFFGLAIILLSLEAARRTVGLGLTIVVLGFMAYNLFGHLLTGPMS
                     HGYISLNHLIDLTVFTSDGLFGVPLQVTATYAFMFVLFGTLLEKAGGGSFFFDMAAVF
                     TGRRVGGPAKVAVMSSAMFGTISGSPTSDVVTTGAVTIPMMKRLGYDDEYASAVEVTA
                     STGGSLLPPVMGSAVFIMAEFTGISYLDIAIACIFPALLYYLGVYIQVHLRSLKLGHQ
                     GLPADRIPGFWATMRSGGLFLIPLVTLVTALVLHYTPTYVALFGVVAVLVAWILRRQN
                     LSFSAVYDAVAHTTFNMVAVTGACAVAGLVIGGITMTGLAGKVSDLILMMAGANMLLS
                     LFIAAACVILLGMGMPTPAAYALAAALIAPTLTKTFEIPVIQAHLFLL"
     gene            complement(1552..2535)
                     /locus_tag="VM406_13930"
     CDS             complement(1552..2535)
                     /locus_tag="VM406_13930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HVL77109.1"
                     /translation="MKQAVLSLALAVGAMNVAHAQLNKTNLTLCGASPGGMWSLVGTG
                     IDSAVKTAYPGSTVTYQTSSGGPANVMQVKAGRCDVGIANDGDLAAANDGRAPFRQKV
                     EGMQAIGVMFDWLPVMWIARKDFAEKHGIKNLEDLAEKKPPVRIAFNRRGLLTSDITD
                     ATLKSLGVSAEDVRKWGGSIQYQASEEQANLMKDGRVDLIANTIFEGHRSLAEIAMST
                     DLVMLNTPPKAAEQVIKDYYVKHWTIKANAHPWQTEDIKTVTTSIVLFADKSMSEQTA
                     YDLTKAILQHPDRVRAVSKAMEPFKPQIMIDQRVVPFHPGAVRAYKEAGLM"
     gene            complement(2954..4141)
                     /gene="tcuB"
                     /locus_tag="VM406_13935"
     CDS             complement(2954..4141)
                     /gene="tcuB"
                     /locus_tag="VM406_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tricarballylate utilization 4Fe-4S protein TcuB"
                     /protein_id="HVL77110.1"
                     /translation="MQQLKTLVNEVSLETSRVIPIKPVALTGNEEEVARQMQICNACR
                     YCEGFCAVFPAMTRRLEFNKADIHYLANLCHNCGACLHACQYAPPHEFGVNVPQAMAK
                     VRLQTYTDFAWPGAFGALYKRNGLTVGLSLAAALSLFLILTVMTTGGLFHEPVAGNFY
                     AIFPHNTLVLMFGSVFGLAVLALAIGVTRFWRAVNPPDFLQLAQAPAYAEAATHAARL
                     TYLDGGHGKGCNNEDDKFTLLRRTFHHFTFYGFMLCFAATSVATLYHYLLGLQAPYPY
                     LSLPVVLGTVGGIGLLIGPAGLLWLNLRRHPEHGDAAQKPMDRGFIVLLFLTSATGLA
                     LLAWRDTSAMGLLLAVHLGVVMALFLTLPYGKFAHGVYRCAALLKSAIEKRQPMRLKL
                     GDD"
     gene            complement(4128..5549)
                     /gene="tcuA"
                     /locus_tag="VM406_13940"
     CDS             complement(4128..5549)
                     /gene="tcuA"
                     /locus_tag="VM406_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HVL77111.1"
                     /translation="MKYDVLVIGGGNAALCAALTAREAGASVLLLEAAPREWRGGNSS
                     HTRNVRCMHDAPQDVLVDAYPEDEYWQDLLKVTGGQTDETLARIAIRASASFRPWMRK
                     HGVNFQPPLSGALHVARTNAFFMGGGKALVNAYYRSAEALGIQIRYNARVDELELQDG
                     RFVAAFVGNERIEAHACVLAAGGFESNREWLREAWGQNQWGEWPADNFLIRGTRFNQG
                     TLIKFMMDAGADIIGDPSQSHCVAIDARAPLYDGGICTRVDCVSLGIMVNRNGERFYD
                     EGEDFWPKRYAIWGRLVAHQPGQIAYCIIDSKAIGRFMPPVFPGVKSDNLPALARQLG
                     LDEAAFMHTVNEYNAACRVGTFDHAVLDDCHTEGLTPPKTHWARPLDTGPYYAYALRP
                     GITFTYLGLKINEESAVHFDGRPSPNLYVAGEMMAGNVLGKGYTAGVGMTIGTAFGRI
                     AGAGAARAAARIAEQQGVEHAAA"
BASE COUNT         1008 a         1754 c         1849 g          944 t
ORIGIN      
        1 cagcaggaac aggtgcgcct ggatgaccgg aatctcgaaa gtcttggtca gcgtcggcgc
       61 gatgagggcc gcagccagcg cataggccgc aggcgtcggc atgcccatgc cgagcaggat
      121 cacgcaggcg gcggcgatga acaggctcag cagcatgttt gcgcccgcca tcatcaggat
      181 caggtccgat accttgccgg ccaggccggt catcgtgata ccgccgatca ccagccccgc
      241 caccgcgcag gcgccggtca cggcaaccat gttgaacgtg gtgtgcgcga cggcgtcgta
      301 gacagcggag aacgacaggt tctggcgacg caggatccag gcgaccagca ccgcgaccac
      361 gccgaacagc gcgacatagg tcggcgtgta gtgcagcacc agcgcggtca ccagcgtaac
      421 cagcggaatc aggaacaggc cgccgctgcg catcgtcgcc cagaagccgg ggatgcggtc
      481 cgccggcagg ccctggtggc cgagcttgag cgagcgcagg tgcacctgga tgtagacgcc
      541 gaggtagtac agcagcgccg ggaagatgca ggcgatcgca atgtcgagat aggagatgcc
      601 ggtgaactcg gccatgatga acaccgccga gcccatcacc ggcggcagca ggcttccacc
      661 ggtgctggca gtcacttcaa ccgcggacgc gtattcatcg tcgtagccca ggcgcttcat
      721 catcgggatc gtgaccgcgc cggtggtcac cacgtcggaa gtcggcgaac cggaaatggt
      781 accgaacatc gccgaggaca tcaccgcgac cttggccggg ccgccgacgc ggcggccggt
      841 gaacaccgcc gccatgtcga agaagaacga acccccgccg gccttctcga gcagcgtgcc
      901 gaacagcacg aacatgaacg cataggtggc ggtcacctgc agcggcacgc cgaacagccc
      961 gtcgctggtg aagacggtca ggtcgatcag gtggttgagc gagatatagc catgcgacat
     1021 cgggccggtc agcaggtggc cgaacaggtt gtaggccatg aagccgagca cgacgatggt
     1081 caggccgaga ccgacggtac ggcgcgcagc ctcgagcgaa agcaggatga tcgcgagacc
     1141 gaagaagaaa tagctgccgg agagctcgtc gaacagagaa atgcgcaccg cgatgttctc
     1201 catttcgctg aagaagaaca ccaggctggc gagcgagagg cccgagagcg catagtcgat
     1261 cagtgccggc ttcgtggtgc tcgagctcgg ccgcgggccg atcagcagga ataccagcac
     1321 cagcatcagg cacaggaaga tgatgatgcg cgccaggttg tcccagctgg agaaggcggc
     1381 accgtagacg gtatagccgg cggtcagcac cgcgtagagt ttcagcgcct ggccaagcca
     1441 gccttccggc ctgcgacgcg cgcccgtgga aaaaatcaag ctggtcaggc tcatcggcct
     1501 atcctcctca ttcgttcgtg cgcgccgcct gcgggcggcg cgcgggtggc attacatgag
     1561 accggcttcc ttgtaggccc ggacggctcc cgggtggaag ggcacgacgc gctgatcgat
     1621 catgatctgc ggcttgaacg gctccatcgc cttcgatacc gcgcgcacgc ggtccggatg
     1681 ctgcaggatc gccttggtca ggtcgtaggc ggtctgctcg gacatcgact tgtcggcgaa
     1741 cagcacgatg ctggtcgtga ccgttttaat gtcctcggtc tgccacggat gagcgttggc
     1801 cttgatggtc cagtgcttga cgtagtagtc cttgatcacc tgctcggccg ccttcggcgg
     1861 cgtgttgagc atgaccagat cggtcgacat cgcgatttcg gccaggctgc ggtggccttc
     1921 gaagatcgtg ttggcgatca ggtccacgcg cccgtccttc atcaggttcg cctgttcctc
     1981 ggacgcctgg tactggatcg atccgcccca cttgcgcacg tcttcggcgc tcacgcccag
     2041 gctcttgagc gtggcgtcgg taatgtccga agtcagcagt ccgcggcgat tgaacgcgat
     2101 gcgcaccggc ggcttctttt ctgcgagatc ctcgagattc ttgatgccgt gcttctcggc
     2161 gaaatccttg cgcgcgatcc acatcaccgg cagccagtcg aacatcacgc cgatggcctg
     2221 catgccttcg accttctggc ggaagggcgc gcggccgtcg ttggcggcgg cgaggtcgcc
     2281 gtcgttggcg atgccgacgt cgcagcggcc ggccttcacc tgcatcacgt tggcggggcc
     2341 gccgctggag gtctggtagg tcaccgtcga accggggtag gcggtcttca ccgccgaatc
     2401 gatgccggtg ccaaccagcg accacatgcc gccgggactt gcgccgcaca gggtcagatt
     2461 ggtcttgttg agctgcgcgt gtgccacgtt catggcaccc acggccaggg ccagcgataa
     2521 gactgcttgc ttcacttgtc tctcctccaa aaaagagcgg ttgatgacaa caacaacaac
     2581 aacttccgtc tgcttctact tattcgattg cttgcagtgg ccgccgcttg ctcgtccaag
     2641 tgtggacgga cgcatgcaag tgtacacaca aaaaaaacat aatctcaagc aacaaaacac
     2701 tgcaagagaa aatgggaaag tccgcctccg gctgggacaa cacgcccttg gcgcactccc
     2761 cgtccgatcc ggcgccccac cgcacgcggt gggagccgat cggccagaga gcagaaggaa
     2821 gttgcttggg agaatgcaaa tgccgtgccc gacattgcca tgctttccgc ggttccttgc
     2881 tgtacgccgg tgcatgggcg gcaccagtgc cgatcactgc cgcaccattg ccggtctttc
     2941 ccgtaaccgt cattcagtcg tctcccagct tcagacgcat cggctggcgc ttttcgatcg
     3001 cgctcttgag cagggccgcg cagcggtaca cgccgtgggc gaacttcccg tacgggaggg
     3061 tgaggaacag cgccatcacc acgccaaggt gcacggcgag cagcaggccc atcgcgctgg
     3121 tgtcgcgcca cgcgagcagc gccaggcccg tggcgctggt gagaaacagc aggacgatga
     3181 agccgcggtc catgggcttc tgcgcggcgt caccgtgctc gggatggcgg cgcaggttca
     3241 gccacaggag cccggccggg ccgatcagca ggccgatgcc gcccacggtg ccgagcacga
     3301 ccggcagtga caggtaggga tacggcgcct gcagcccgag caggtagtgg tacagcgtgg
     3361 cgacgctggt tgccgcgaag cacagcatga agccgtagaa cgtgaagtga tggaaggtgc
     3421 ggcgcagcag cgtgaacttg tcgtcctcgt tgttgcagcc cttgccgtgg ccgccgtcta
     3481 ggtaggtcag gcgcgccgcg tgcgtggccg cttccgcata ggccggcgct tgcgcgagct
     3541 gcaggaaatc gggcgggttg accgcgcgcc agaagcgcgt gaccccgatc gcgagcgcga
     3601 gcaccgcgag accgaatacg ctgccgaaca tcagcacgag tgtgttgtgc gggaagatcg
     3661 cgtagaaatt gccggccacc ggctcatgga acagtccgcc ggtggtcatg acggtcagga
     3721 tcaggaacag cgacagcgct gccgccagcg acaggccgac ggtcaggccg ttgcgcttgt
     3781 agagcgcgcc gaacgcgccc ggccatgcga aatcggtgta ggtctgcagg cgcaccttcg
     3841 ccatcgcctg cggcacgttg acgccgaact cgtgcggcgg cgcgtactgg caggcatgca
     3901 ggcaggcgcc gcagttgtgg cacaggttgg cgaggtagtg gatgtcggcc ttgttgaact
     3961 ccaggcggcg cgtcatcgcc gggaacaccg cgcagaagcc ttcgcagtag cggcaggcgt
     4021 tgcagatctg catctggcgc gcgacctctt cctcgttgcc ggtcagcgcg acgggcttga
     4081 tcggaatgac ccggctcgtc tccagactga cttcgttgac gagggtttta agctgctgca
     4141 tgttcgactc cttgctgttc tgcgatgcgc gccgcggcac gcgcggcgcc cgcgccggcg
     4201 atgcgaccga acgcggtgcc gatcgtcatg ccgacgccgg ccgtgtatcc cttgccgagc
     4261 acgttcccgg ccatcatttc gcccgcgaca tagaggttcg ggctcggccg gccgtcgaag
     4321 tggacagccg actcctcgtt gatcttcagg ccgaggtagg tgaaggtgat gcccggccgc
     4381 agcgcgtacg catagtaggg accggtatcg agcggacgcg cccagtgcgt cttcggcggt
     4441 gtcagtcctt cggtatggca gtcgtcgagc accgcgtggt cgaaggtgcc gacgcggcag
     4501 gccgcgttgt attcgttcac ggtgtgcatg aacgcggctt cgtcgagtcc gagctggcgc
     4561 gccagcgcag gcaggttgtc ggacttcacc ccggggaaga ccggcggcat gaagcggccg
     4621 atggccttgc tgtcgatgat gcagtaggcg atttggcccg gctggtgcgc gaccaggcgg
     4681 ccccagatcg catagcgctt cggccagaag tcctcgccct cgtcgtagaa gcgctcgccg
     4741 ttgcggttga ccatgatgcc gagcgagacg cagtccacgc gggtgcagat gccgccgtca
     4801 tagagcgggg cgcgcgcgtc gatggccacg caatgcgatt gcgacgggtc gccgatgatg
     4861 tcggcgccgg catccatcat gaatttgatc agcgtgccct gattgaagcg ggtaccgcgg
     4921 atcaggaaat tgtcggccgg ccattcgccc cactggtttt ggccccaggc ttcgcgcagc
     4981 cactcgcggt tcgattcgaa tccgcccgcg gccagcacgc aggcatgtgc ctcgatgcgt
     5041 tcattgccga cgaaggcggc gacgaaacgg ccgtcctgca gttccagttc gtcgacgcgc
     5101 gcgttgtagc ggatctggat gccgagcgct tcggcactgc ggtaataggc atttaccagc
     5161 gccttgccgc cgcccatgaa gaacgcattg gtgcgcgcca catgcaatgc accggatagc
     5221 ggcggctgga agttcacccc gtgcttgcgc atccacggac ggaagctcgc ggaggcgcgg
     5281 atcgcgatgc gcgccagcgt ttcatcggtc tgcccgccgg tgacctttag cagatcctgc
     5341 cagtactcgt cttcgggata ggcgtcgacc agcacgtcct gcggcgcatc gtgcatgcag
     5401 cgcacattgc gcgtatgcga cgagttcccg ccgcgccact cgcgcggcgc cgcctccagc
     5461 agcagcaccg aggcgcccgc ttcgcgggcc gtgagcgctg cgcacagggc ggcgttgccg
     5521 ccgccgatca ccaacacgtc atatttcatt catca
//
LOCUS       DATLGF010000454         5630 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_142394, whole genome shotgun sequence.
ACCESSION   DATLGF010000454 DATLGF010000000
VERSION     DATLGF010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142394"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..92)
                     /locus_tag="VM406_13945"
     CDS             complement(<1..92)
                     /locus_tag="VM406_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HVL77112.1"
                     /translation="MRIGIVSDIHGNLAALEAVVADMRRRGVDQL"
     gene            complement(138..623)
                     /locus_tag="VM406_13950"
     CDS             complement(138..623)
                     /locus_tag="VM406_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVL77113.1"
                     /translation="MSTPAKNTICLWYDGDAEEAAQFYARTFPDSSVGAVYRAPGDFP
                     SGKQGDVLTVWFTVMGIPCLGLNGGPAFKHSEAFSFQVATVDQAETDRYWNAIVGNGG
                     QESACGWCKDKWGLSWQITPIALTEAIADPDPAAAKRAFDAMMQMTKIDIAAIEAARR
                     G"
     gene            complement(989..3301)
                     /locus_tag="VM406_13955"
     CDS             complement(989..3301)
                     /locus_tag="VM406_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HVL77114.1"
                     /translation="MDSSSDQKEQIRQQLRQSALEYHEFPTPGKISVTPTKQLTNQRD
                     LALAYSPGVAAACEEIVADPVNAYRYTARGNLVAVITNGTAVLGLGNIGPLASKPVME
                     GKGVLFKKFAGIDVFDIEINENDPDKLIDVIAALEPTFGGVNLEDIKAPECFYIERKL
                     RERMKIPVFHDDQHGTAIIVGAAVLNGLKVVGKQIEDCKLVVSGAGAAALACLDLLVD
                     LGFPLKNIFVTDLAGVVYKGRKELMDPDKERFAQETDARSLAEVIPGADIFLGLSAGG
                     VLKREMVAQMNDRPLILALANPTPEILPEEVKAVRNDAVIATGRSDYPNQVNNVLCFP
                     YIFRGALDCGATTITREMEIAVVHAIAELAQAEQSDIVATAYGISNLSFGPEYVIPKP
                     FDPRLMIKIAPAVAKAAEDGGVASRPIKDMQAYIDSLQQFVYRSGTFMKPLFQIAKKA
                     PPEKKRIVFAEGEEERVLRAVQVVIDERLAKPILIGRPAVLAQRIEKFGLRMRPGVDF
                     TVINPEYDERYRDYWQEYLALTRRKGISEQYAKLEMRRRHTLIGAMTVRKGDADGMIC
                     GTYGTVPIHLQYIDQVLGKREGVNVYAAMNALILPDRQIMLVDTHVNENPTAEQIAEI
                     TLLAAEEMRRFGVEPRAALLSHSNFGTSNSESAQKMRRALAILRDMAPDLEVDGEMHG
                     DTALDAAVLKKTMPDSPLTRDANLLVLPNIDAANIAYNLLKVAAGNGIAIGPILIGCD
                     RPVHVLTPSATVRRIVNMTALCVVDAVSER"
     gene            3532..4008
                     /locus_tag="VM406_13960"
     CDS             3532..4008
                     /locus_tag="VM406_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HVL77115.1"
                     /translation="MAHQQQSVTIQNLEAAFAGESMAHIKYRYFAKLAREAGAEDVAR
                     IFEETAEQEVMHAFGHLDLLYPKARLSPAKALEIAIEGETYEYTEMYPKFRHLAVQEG
                     NEAAVAEFDEQIAESKEHAEAFRRMLEVAAKRFAALAKVEERHANNYRNVLAATAK"
     gene            4036..4200
                     /locus_tag="VM406_13965"
     CDS             4036..4200
                     /locus_tag="VM406_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HVL77116.1"
                     /translation="MKTYQCIVCGFIYDEAAGMPEDGIAPGTRWEDIPADWECPDCGV
                     AKADFEMVEI"
     gene            4226..5407
                     /locus_tag="VM406_13970"
     CDS             4226..5407
                     /locus_tag="VM406_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVL77117.1"
                     /translation="MKPTIIIGSGLAGYTVAREVRKLDKNKPLLIVTADDGGFYSKPM
                     LSNAYAHGKEAAQLVSQTAAQMASQLGASILTGTRVSGLDMAAATVATEAGSFEYEQL
                     VLALGAQPIRLALNGDAAHEVVSVNTLTDYAALRARIAGAGARARVLILGAGLIGCEF
                     ADDLAGAGHIVTLVDPNPMPLASLAPPALSQGLAAALRQRGVDLQLGTTATRVERGAD
                     GYQVLLNDGQLYHADVVLSAVGLRPELSLAQAAGLNTRRGIVVDAYGRTSAQDVYALG
                     DCAEYADGAGSVCLPYIAPLMTAARAIARTVAGTPTAIDMKAAPVIVKTPSYPLALLP
                     PAAALRETGAWYTARDGERTICRFCDADGVVRGFGVAPQDAVSRQALMAEVGNAMALA
                     A"
BASE COUNT         1000 a         1775 c         1833 g         1022 t
ORIGIN      
        1 agctggtcca ctccgcgtcg ccgcatatcg gcaacgactg cttccagagc cgcgaggttg
       61 ccatgaatgt ccgagacgat tcctattcgc atagcatcga cttcgttgcg tgattaaccg
      121 gtcggcatga gcggcgctca accgcggcgc gccgcctcga tcgccgcgat gtcgatcttt
      181 gtcatctgca tcatcgcatc gaacgcgcgt ttggcggcag cgggatcagg atcggcgatc
      241 gcttccgtca gcgcgatcgg cgtaatctgc caggacagtc cccatttgtc cttgcaccag
      301 ccgcaagcgc tctcctggcc gccgttgccg acaattgcgt tccagtagcg atcggtttcg
      361 gcctggtcga cggttgcgac ctgaaacgag aaggcttcgc tgtgcttgaa cgcggggccg
      421 ccgttcagcc ccaggcacgg aatccccatg acggtgaacc agacggtcaa cacgtcccct
      481 tgcttccccg aaggaaaatc cccaggagcg cggtacacgg cgccgacgga cgagtcggga
      541 aaggttctgg cgtaaaactg cgccgcctcc tcggcgtcgc catcgtacca aaggcagatc
      601 gtgttctttg cgggtgttga catgtcactc ctccgttgtg atgggttgcc cgacaccgga
      661 cctgcaggat gaatgcatga accaggccgc gcagcgaagc ggggtcggct tgaatggcgg
      721 ggcgaagcga agcatggagg gcttgcggtc ctgcctaccg tactgttcgc atgagcgcct
      781 gagggttgtg ttaagccaca tcagagacag cgtcggtttg gacgaagtgc cattcaccgc
      841 accatcttac cggccaagtc ggtagagcag caggcgttag gcggcacgat gacaagtcag
      901 gcacccgtgc catgcatgcc caaaaaagcg aaacggccgc ccaagggcgg ccgttgctcg
      961 aatcataccg gtctggccgg cacccgggct accgctccga cactgcatcc accacgcaca
     1021 gcgcggtcat gttcacgatg cggcgcacgg tggccgacgg ggtcagcacg tgcaccgggc
     1081 ggtcgcagcc gatcaggatg ggcccgatcg cgatgccgtt gccggcggcg accttgagca
     1141 ggttgtaggc gatgttggct gcgtcgatgt tcggcagcac cagcaagttg gcgtcgcggg
     1201 tgagcggcga atccggcatc gtcttcttca acacggcggc gtcgagcgcg gtgtcgccgt
     1261 gcatctcgcc gtcgacttcg agatccggcg ccatgtcgcg caggatcgcc agcgcacggc
     1321 gcatcttctg cgcggattcg ctgttgctgg tgccgaagtt cgagtgcgac agcagcgccg
     1381 cgcgcggttc cacgccgaag cggcgcatct cttcggcggc caggagcgtg atctcggcga
     1441 tctgctcggc ggtcgggttc tcgttgacgt gggtgtccac cagcatgatc tggcgatccg
     1501 gcaggatcag cgcattcatc gccgcataga cattcacgcc ctcgcgcttg ccgagcacct
     1561 ggtcgatgta ctgcaggtgg atcggcacgg tgccgtaggt gccgcagatc atgccgtcgg
     1621 catcgccctt cctgaccgtc attgcgccaa tcagcgtgtg gcggcgacgc atctccagct
     1681 tcgcgtactg ctcggagatg cccttgcggc gggtgagcgc caggtattcc tgccaatagt
     1741 cgcggtagcg ttcgtcgtac tcgggattga tcaccgtgaa atcgacgccc ggacgcatgc
     1801 gcagtccgaa tttttcgatg cgctgcgcca gcacggccgg tcggccgatc aggataggct
     1861 tggccagcct ttcgtcgatg accacctgca ccgcgcgcag cacgcgctct tcctcgcctt
     1921 cggcgaacac gatgcgcttc ttctccggcg gcgccttctt cgcaatctgg aacagcggct
     1981 tcatgaaggt gccgctgcgg tagacgaatt gctgcaggct gtcgatgtag gcctgcatgt
     2041 ccttgatcgg gcgcgacgcg acgccgccgt cttccgccgc cttcgccact gcaggcgcga
     2101 tcttgatcat caggcgcgga tcgaagggct tcggaatcac gtattccggg ccgaacgaga
     2161 gattgctgat gccgtaggca gtggcgacga tgtcggactg ctcggcctgg gccagttcgg
     2221 caatcgcgtg cacgaccgcg atctccatct cgcgcgtgat cgtggtcgcg ccgcagtcga
     2281 gcgcgccgcg aaagatgtac gggaagcaga gtacgttgtt gacctggttc gggtagtcgg
     2341 agcggccggt cgcgatcacc gcgtcgttgc gcaccgcctt gacttcctcg ggcaggattt
     2401 ccggggtcgg gttggccagc gcgaggatca gcggccggtc gttcatctgc gcgaccatct
     2461 cgcgcttgag cacgccgccg gcggacaggc cgaggaagat gtcggcgccg gggatcacct
     2521 cggcgagact gcgcgcatca gtctcctgcg cgaagcgctc cttgtccgga tccatcagtt
     2581 ccttgcggcc cttgtagacc acgccggcca ggtccgtcac gaagatgttc ttcagcggga
     2641 agccgaggtc gaccagcagg tccaggcagg ccagcgcggc ggcgccggcg ccggagacaa
     2701 ccagcttgca gtcctcgatc tgcttgccga ccaccttcag gccgttgagg acggccgcgc
     2761 cgacgatgat cgcggtgccg tgctggtcgt catggaagac cgggattttc atccgctcgc
     2821 gcagcttgcg ctcgatgtag aagcactcgg gcgccttgat gtcttcgagg ttcacgccgc
     2881 cgaaggtggg ctcgagcgcg gcgatcacgt caatcagttt gtccgggtcg ttctcattga
     2941 tctcgatgtc gaacacgtcg atgccggcga acttcttgaa cagcacgccc ttgccttcca
     3001 tcaccggttt ggatgcgagc ggcccgatgt tgccaagtcc cagcaccgcc gtaccgttgg
     3061 tgatcaccgc gaccaggttg ccgcgggcgg tgtaacggta ggcattgacc ggatcggcga
     3121 cgatttcctc gcaggccgcg gccacccccg gcgagtaggc aagcgcgagg tcgcgctggt
     3181 tggtcagctg cttggtcggc gtgacgctga ttttcccagg ggtcggaaac tcatggtatt
     3241 cgagcgcgga ttgccgcagt tgctggcgga tttgctcttt ttgatcgctc gacgagtcca
     3301 tggcgcattc cctggttgct tgcagaaagg cgacgatttt atcagagggg tatcggtcga
     3361 cttatacgta tttgtcctaa cgtcatgctg ccctgagata aggaaatcca gccgagaaaa
     3421 acagaaaagc tatcgaatcg atcaaaacgt tcaatttcat ttattaatca cgttctccta
     3481 ttattcttct cgtattgatt aattaaacct aacagagaaa gggagcacac catggcccac
     3541 caacaacaat ccgtgaccat ccagaacctc gaagcggcat tcgccggcga gtcgatggcc
     3601 catatcaagt accgctactt cgccaagctg gcgcgcgagg caggcgccga ggacgtggcg
     3661 cgcatcttcg aagagactgc cgagcaggaa gtgatgcatg cattcggcca cctcgacctg
     3721 ctctacccga aggccaggtt gagcccggcc aaggcgctgg agatcgcgat cgagggcgag
     3781 acctacgagt acaccgagat gtatccgaaa ttccgccacc tggcggtgca ggaaggcaat
     3841 gaagcggcgg ttgccgagtt cgacgagcag atcgccgaat cgaaggaaca tgccgaggca
     3901 tttcgccgca tgctggaagt cgccgccaag cgcttcgccg cgctggccaa ggtcgaggag
     3961 cgccacgcaa acaactaccg taacgtgctc gccgcaaccg ccaagtaaac caacaacgat
     4021 ccaggaagga aaaccatgaa gacttatcag tgcatcgtat gcggcttcat ctacgacgag
     4081 gcggccggca tgccggagga cggcatcgcg cccggcaccc gctgggaaga cattccggcc
     4141 gactgggaat gcccggactg cggcgtggcc aaggccgact tcgagatggt ggaaatctga
     4201 tcccccgccg cccgcatacg aaaacatgaa acccacgatc atcatcggaa gcgggctggc
     4261 cggctacacg gtcgcccgcg aagtccgcaa gctcgacaag aacaagccgc tgctcatcgt
     4321 cacggccgac gacggcgggt tttactccaa gccgatgctg tccaacgcct acgcccatgg
     4381 caaggaggcc gcgcagctgg tctcccagac ggcagcccag atggcctccc agctgggtgc
     4441 aagcatcctg accggcaccc gcgtgtcggg ccttgacatg gcggccgcaa cggtggcaac
     4501 cgaggccggc agcttcgagt acgaacaact ggtgctggcg ctcggcgcgc agccgatccg
     4561 gctggccctg aacggggacg ccgcgcacga ggtcgtgtcg gtgaacacgc tgacggacta
     4621 cgcggccctg cgcgcacgca tcgctggcgc cggcgcgcgt gcacgggtgc tgatccttgg
     4681 cgcaggcttg atcggctgcg agttcgccga cgacctggcc ggcgctggcc atatcgtcac
     4741 gctggtcgac ccgaatccga tgccgctggc ctcgctcgcc ccgcccgcgc tatcgcaggg
     4801 gctggcggcg gcgctgcgac aacgcggcgt cgacctgcag ctcggcacga cggcgacgcg
     4861 cgtcgagcgc ggcgccgacg ggtatcaggt cctgctcaac gacggccagc tctatcatgc
     4921 ggacgtggtg ctgtccgctg tcggcctgcg gccggagctg agcctggccc aggctgcggg
     4981 cttgaacacg cggcgcggca tcgtcgtcga tgcctacggc cgcaccagcg cgcaggacgt
     5041 gtacgcgctg ggcgactgcg ccgagtatgc ggacggcgcg ggttccgtct gcctgcccta
     5101 catcgcgccg ctgatgacgg cggcgcgcgc gattgcgcgc accgttgccg gcacgccgac
     5161 ggcgatcgac atgaaggccg caccggtcat cgtcaagaca ccgagctatc cgctcgcgct
     5221 gctgccgccg gcggccgcgc tgcgcgagac aggtgcgtgg tacacggcgc gcgacggcga
     5281 acgcacgata tgccggttct gcgatgccga cggtgtggtg cgtggcttcg gggtggcgcc
     5341 gcaggatgcg gtgagccggc aggcgctgat ggcggaagtg ggtaatgcga tggcgctggc
     5401 ggcgtaggcg ggctggggcg atagagtttt cctggcgagt tcacgcggac tcgctctgtt
     5461 tgacttcgga actgggttcc cgcctgagcg ggggcccgcc taggggaacg acagttttct
     5521 ggacgccgct ctttttcagt ttgaacggcc aagccgttga aaagcacggc cactcccgca
     5581 cgtcgttccc gcgcaggcgg gaacccagtt ccgaagtcaa acagagcgag
//
LOCUS       DATLGF010000455         2387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_105670, whole genome shotgun sequence.
ACCESSION   DATLGF010000455 DATLGF010000000
VERSION     DATLGF010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105670"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..1658
                     /locus_tag="VM406_13975"
     CDS             126..1658
                     /locus_tag="VM406_13975"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HVL77118.1"
                     /translation="MDAPQTLTGIVDWLRKIAPAARLASDSRTIGAGDVFVAYPGEAS
                     DGRRYIAAAIERGAAAVLYDARGFEWDDAWQVPHLAVGELKQQVGPIAGSLLGMPDAE
                     MFSIAVTGTNGKTSCSQWLGSALSRLGMPTAVIGTLGIGLYRGGSHGGLHATGLTTPD
                     AAQLQYELARLRDSGAKALAIEASSIGLHQGRLNGMHFDVAVFTNFTRDHLDYHGDMA
                     SYEAAKRMLFDWPQLKHGVINLDDEAGRRLAEHLRRNAPGVQVLGYSLNGCNLPGVAT
                     LRAADVRAHHGGTAFRLESLFGNTQVRTQLVGQFNVSNVLAILGVLIAKGVRFKDAIG
                     AVEALTPVPGRMQQVGGIDAPLVVVDYAHTPDALEQALKALRQVAGQRGGQLWCLFGC
                     GGDRDPGKRAQMGAVSQLADHIVVTSDNPRSEEPGAIIAQIVAGIAPQRAAAGNLHII
                     EDRAGAILWAVRHAGRQDVVLLAGKGHEAYQEIKGRKMPFLDADHAALALASRATMKG
                     SN"
     gene            1658..>2387
                     /locus_tag="VM406_13980"
     CDS             1658..>2387
                     /locus_tag="VM406_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HVL77119.1"
                     /translation="MKSSLAQLQPAISQAVATADAAFDGVCTDSRSVAPGNLFVALRG
                     ERFDAHDFLADVAARGAAAVVVERVPQGLQVPALVVPDTRTALGDIGRWWRSRFALPV
                     VGVTGSNGKTTVKEMTAAILSAAHGPDQVLATRGNLNNDIGVPLTVFRLRAGCKAAVI
                     ELGMNHPGEIAVLTRIARPTVALVNNAQREHQEFMATVEAVARENGSVIEGLDADGVA
                     VFPADDAYTAIWRELAGTRRTLTFG"
BASE COUNT          391 a          769 c          855 g          372 t
ORIGIN      
        1 ggacaggtgg ccgcgccggt gttcgcggca gtggtcggca gcgcgctgcg cgcgttgaac
       61 gtgccgccgg attcgaacgt caccgacatc atcattccgg ccgagcccgt gcaggagagt
      121 atctagtgga tgcgccgcaa acactgaccg gcatcgtcga ctggctgcgc aagatcgcgc
      181 cggctgcgcg gttggcatcc gactcgcgca cgatcggcgc gggcgacgtg ttcgttgcgt
      241 atccgggcga ggccagcgac ggacgtcgct acatcgccgc ggcgatcgaa cgcggtgcgg
      301 ccgcggtgct gtacgacgcg cgcggcttcg agtgggacga cgcatggcag gtgccgcacc
      361 tggcagtcgg cgagctgaag cagcaggtcg gaccgattgc cggcagcctg ctcggcatgc
      421 cggacgcgga gatgttctcg atcgcggtca ccggcaccaa tggcaagacg tcctgttcgc
      481 agtggctggg cagtgcgttg tcgcgcctgg gcatgccgac ggcggtgatc ggcacgctcg
      541 gcatcggcct gtatcgcggc ggcagccatg gcggcctgca tgcgaccggg ctgaccacgc
      601 cggatgccgc gcagctccag tacgagctgg cgcgtctgcg tgacagcggg gcgaaggcgc
      661 tggcgatcga ggcgtcttcg atcggcctgc atcagggccg cctgaacggc atgcatttcg
      721 atgtagcggt atttaccaac ttcacgcgcg accacctcga ctatcacggc gacatggcgt
      781 cctacgaagc ggccaagcgc atgctgttcg actggccgca actcaagcac ggcgtgatca
      841 atctggatga cgaggcgggc cgcaggctgg ccgagcacct gcgcaggaat gcgccggggg
      901 tgcaggtcct cggttacagc ctgaacgggt gcaacctgcc cggcgtggct acgctgcgcg
      961 ccgccgatgt gcgcgcccac catggcggca ccgcattccg gctggagtcc ctgttcggca
     1021 acacgcaggt caggacgcag ctggtcggcc agttcaacgt cagcaacgtg ctggcgatcc
     1081 tgggtgtgct gattgctaag ggcgtgcgct tcaaggacgc catcggcgcg gtcgaggcgc
     1141 tcacgccggt gcccggacgc atgcagcagg tcggcggcat tgacgcgccg ctcgtggtgg
     1201 tcgactacgc gcatacgccg gatgcgctcg agcaggcact caaggcgctg cgccaggtcg
     1261 caggacagcg cggcggccag ctgtggtgcc tgttcggctg cggcggcgac cgcgatccgg
     1321 gcaagcgcgc gcagatgggc gcggtctcgc agctggccga tcacatcgtg gtcaccagcg
     1381 acaacccgcg cagcgaagag cccggcgcca tcatcgcgca gatcgtggcc ggcatcgcac
     1441 cccagcgcgc cgcggcgggc aatctgcaca tcatcgaaga ccgggccggc gccatcctct
     1501 gggcggtgcg gcatgccggt cgacaggacg tcgtcctcct ggctggaaaa ggccatgagg
     1561 cttaccagga aatcaagggc aggaagatgc ccttcctgga tgcggaccac gccgcgctgg
     1621 cgctggcttc gcgagcaaca atgaagggaa gcaactgatg aaatcctcgt tggcgcaact
     1681 gcagccggcg atctcgcagg cggtggcgac agccgatgcg gcgtttgacg gtgtctgtac
     1741 cgacagccgc agcgtcgcgc ccggcaatct gttcgtcgcg ctgcgcggcg aacgtttcga
     1801 cgcgcatgat ttcctcgcag acgtggcggc acgcggtgcg gcagccgtcg tcgtggaacg
     1861 cgtgccgcaa ggcttgcagg tgccggcact ggtggtgcca gatacccgta ccgcgctcgg
     1921 cgatatcggg cgctggtggc gcagccggtt cgctttgccc gtggtcggtg tcaccggcag
     1981 caatggcaag accacggtca aggaaatgac cgcggcgatc ctgtcggcgg cgcacgggcc
     2041 cgatcaggtg ctggccacgc gcggcaatct gaacaatgac atcggcgtgc cgttgaccgt
     2101 gttccggctg cgcgcaggct gcaaggctgc cgtgattgaa ctggggatga accatccggg
     2161 cgagatcgcg gtgctgaccc gcatcgcccg cccgacggtt gcgctggtca acaacgcgca
     2221 gcgcgaacac caggagttca tggcgaccgt ggaagcggtc gcgcgggaga acggctcggt
     2281 gatcgagggc ctggacgccg acggcgtcgc cgtcttcccc gccgatgacg cttataccgc
     2341 gatctggcgt gaactggccg gcacacggcg cacgctcaca ttcggac
//
LOCUS       DATLGF010000456         7771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_77678,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000456 DATLGF010000000
VERSION     DATLGF010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 7771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7771
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77678"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1734)
                     /locus_tag="VM406_13985"
     CDS             complement(22..1734)
                     /locus_tag="VM406_13985"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HVL77120.1"
                     /translation="MKKILATSLIAALAACGGSNNEEVVASAAMQGGPATPAVPATPA
                     QPGAAPATPATPATPAQPDVPPGEPGAPPATPTQPDENAAALIEAYQGGLGDIALAQL
                     ATQNAQNADVQRLAQRLVDHHTWLNEQWAQLAADQNVTLPTEPGAGFADEQAALQGLS
                     GAEFDQAFLTRTIERHRQLLTQLREQAGLAVTDTNGTNGTNGTNGTNGTNGTNGTNGT
                     APGAPANGEDGEISPAQASRDLAMNAIPAVQTHLEAAKTIAGASNPETFIQQAYVGGL
                     AEIATATLAAERSENEAVRDFAEQMIEDHVRANIEIALLAELEGITLEQTVPPEVRAA
                     LDDLARMSEEDFDKAYMNFNVLAHESLVAQARAQAANGGDPTVTAYAARVLPTLDEHR
                     EMARSIYEDVEPNLLFGAVASGWTEILLSQLAVQVSDNQQVQQFAQRMIDEHTAANRR
                     AMQLAQANDRDLPTQVPAEAALQYIRLSQLTGAEFDREFADQNVAFHEDTLELFRNRQ
                     ADETEQQIQTFASETIPVLEDHLQDARTLAGQINNNAANNGTGNNNGTGNNNDTANSN
                     ATAS"
     gene            complement(2111..2650)
                     /locus_tag="VM406_13990"
     CDS             complement(2111..2650)
                     /locus_tag="VM406_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HVL77121.1"
                     /translation="MPKSTLAGHPLHPMLIVAPAALIPFGFLLDAMHRATGKESYSEA
                     AYYSLMGGLVGGIAAGAAGALDYLAIPRETEVKRTANVHALLNSGALAMTAANVLARK
                     RDGAHRGGSLAASALAAAAVIVSGWFGGRLVYEQGMRVKSVSPVADEPELLIPGDARI
                     AQAMLAAERRMPAAGPVLH"
     gene            complement(2721..3419)
                     /locus_tag="VM406_13995"
     CDS             complement(2721..3419)
                     /locus_tag="VM406_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HVL77122.1"
                     /translation="MIELRKAQERGHANHGWLDSWHSFSFADYYDPKHMSFGPLRVIN
                     EDRVAGGAGFGTHGHRDMEIISYVLDGELAHRDSIGNGSVIRPGDVQRMSAGRGIMHS
                     EYNHAQDRQTHFLQIWIEPNRYGITPGYEEKRFSDDEKRGRLRLIASPDGRDGSVVIH
                     QDAYVYAGLFDGAEQASLPLAAGRRCYVHLVRGRLGVAGHALAAGDALKAEGETAIAL
                     RDGEGAEVLVFDLP"
     gene            complement(3465..4340)
                     /locus_tag="VM406_14000"
     CDS             complement(3465..4340)
                     /locus_tag="VM406_14000"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HVL77123.1"
                     /translation="MQVNANSSTTSPAASGGLRQRLEAFINNGRVQGIILALIAINAV
                     ILGLETSPAVMAEYGTLLVGVDRVILAVFVVEILIRLYVYRLAFFRDPWSIFDFIVVG
                     IALVPATGQLSVLRALRVLRVLRVMTIVPSMRKVVGALLGAIPGLSSIALVLLLIYYV
                     FAVIATNLFAASFPDWFGDLGRSFYTLFQVMTLESWSMGISRPVMEVYPYAWLFFIPF
                     ILIATFSMLNLFIAVIVNAMQTVSDAEHEETRDVVGQTGEHLERELHVEVQALREEIR
                     ALHKLIQSGKGRDQA"
     gene            complement(4418..5341)
                     /locus_tag="VM406_14005"
     CDS             complement(4418..5341)
                     /locus_tag="VM406_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVL77124.1"
                     /translation="MEEPSLSVAGRRTALRGEDNLRSIAFMLAAVAMFALMDTVMKLL
                     AQHYPAMQVAALRGLAALPLVCIWVWWRGATASLRRVRWPLHVLRAGTGILMLFLFAY
                     ALRTLPLAEAYTLAFIAPLLITALSALVLGERIGAARWWAVAGGLVGVLIVLRPTGAG
                     MLSLGAATVLGSAACYAVVAITVRVLARTDSSESIVFWFTAALAGGAGLLALPNWVAL
                     AGAHWPLLIALAITGFLGQILITEAFRRGEASAVAPFEYSALAWAAMLDWMLWQVLPD
                     RYVLAGAVVIVASGVLLLRTEGARVRRDRAG"
     gene            complement(5347..5757)
                     /gene="mscL"
                     /locus_tag="VM406_14010"
     CDS             complement(5347..5757)
                     /gene="mscL"
                     /locus_tag="VM406_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HVL77125.1"
                     /translation="MGMLAEFKTFALRGNVMDLAVGVIIGAAFGRIVDSLVTDLIMPV
                     VGLAVGGLDFSNYFIPLAGQQAATLAEARAGGAVLAYGSFLTVALNFLILAFVIFLMV
                     RQMNRLRAQTPPAPPAAPPEDVLLLREIRDALKR"
     gene            complement(5793..6440)
                     /locus_tag="VM406_14015"
     CDS             complement(5793..6440)
                     /locus_tag="VM406_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HVL77126.1"
                     /translation="MPTRLKIDFVSDVSCPWCAVGLAGLEHALQRVQPEIAAQIHFQP
                     FELNPQMGPEGEDITEHLVAKYGISEAQVEENREAIRARGEQVGFTFNMEKRSRIYNT
                     FDAHRLLHWAQLEGHQAALKHALLKAYFTDGEDPSDHEVLARTAAACGLDEERARAIL
                     GSDAYADETRAQQRFYLEQGIHSVPAVIVNDRYLIQGGQPPEVFERALRQIAEQN"
     gene            6547..6747
                     /locus_tag="VM406_14020"
     CDS             6547..6747
                     /locus_tag="VM406_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77127.1"
                     /translation="MRVQVIHEDRNGAKTDIGMFDLNHMPPVGEPFRVNPNTCFSCKA
                     YFGPDEKGMYLLVLEGEPRMLD"
     gene            complement(6779..>7771)
                     /locus_tag="VM406_14025"
     CDS             complement(6779..>7771)
                     /locus_tag="VM406_14025"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HVL77128.1"
                     /translation="PPRCAASIRPHAPAAAREDGWMQRSVADYEFTSWLFASENHPDL
                     FTNPCEDAILAAIDEAVKSPRSGTHLVRRIPGVIPQLLQSLRSPDFSGQELARLIWHD
                     VVLVSEVLRLANSAAYNPGTPITSIDHAILILGQDGLRQLITGVAFKPIIDMKSGSFT
                     RRVAPHLWDQAERCALANRILARAAGADPLEAFLAGLIHNVGLIVALRIIDQIYDRSQ
                     PLGSPTFCNALVPLGRALTANIAREWHFPDTVIRAVGEADAAEKGGGAGSREPMSALG
                     AILVTGDYLAKLDTLRRQGRIAQDDPRVSHDLSEAQRECFAQIALLEQRDWGLA"
BASE COUNT         1370 a         2527 c         2525 g         1349 t
ORIGIN      
        1 cggatgcacg gcgagtgccg gtcagctggc ggtggcgttg ctgttcgcag tgtcgttgtt
       61 attgccagtg ccgttgttgt tgccagtgcc gttgttggcc gcattgttgt tgatctgccc
      121 ggccagcgtg cgcgcgtctt gcagatggtc ctcgagaacc gggattgttt cggaggcaaa
      181 cgtctggatc tgctgctccg tctcatccgc ctggcggttg cggaacagct ccagcgtgtc
      241 ctcatggaac gccacgttct ggtcggcgaa ctcgcggtcg aactccgcgc cggtcagctg
      301 cgacagacgg atgtattgga gcgccgcttc ggccggcacc tgcgtcggca gatcgcgatc
      361 gttcgcttga gcgagttgca tcgctcgcct gttcgccgcc gtgtgttcat cgatcatgcg
      421 ctgggcgaat tgctgaacct gctgattgtc ggacacctgc accgcgagct gagacagcag
      481 gatctcggtc catccgctgg cgaccgcgcc gaacagcagg tttggctcga catcctcata
      541 gatgctgcgt gccatctcgc gatgttcgtc aagcgtgggc agcacacgcg cggcataggc
      601 cgtcacggtc ggatctcccc cgttggcggc ctgcgccctg gcctgcgcga ccagcgattc
      661 gtgcgcgagc acgttgaagt tcatgtaggc cttgtcgaag tcctcctcgc tcatgcgagc
      721 cagatcgtcg agcgcggcgc gcacctccgg cggaaccgtc tgctcgagcg tgattccctc
      781 cagttcggcc agcagcgcga tctcgatgtt cgcacgcacg tggtcctcga tcatctgttc
      841 ggcgaagtcg cgcaccgcct cgttttccga acgctcggcg gccagtgtcg ctgtcgcgat
      901 ttcggcgagg ccgcccacgt aggcctgctg gatgaaggtc tcgggattgc tcgcgccggc
      961 aatggtcttg gccgcttcca gatgggtctg cacagcggga atggcgttca ttgccaggtc
     1021 gcggctggcc tgggcgggcg aaatctcgcc atcctcgcca ttggcgggcg cgccgggtgc
     1081 ggtgccgttg gtgccattgg tgccattggt gccattggtg ccgttggtgc cgttggtgcc
     1141 gttggtgccg ttggtgtcgg tcacagccag tcctgcctgc tcgcgcaact gagtcagcaa
     1201 ttggcgatgg cgttcgatcg tccgcgtcag gaatgcttgg tcaaactctg caccgctcaa
     1261 gccttgcaat gccgcctgtt cgtcggcaaa gcctgcaccg ggttcggtgg gcagcgtcac
     1321 gttctgatcg gccgcgagct gcgcccactg ctcattgagc caggtgtggt ggtcgacgag
     1381 gcgttgcgcc agtcgctgca cgtcggcatt ctgcgcgttc tgcgtcgcca gttgggcaag
     1441 ggcgatgtcg ccgagcccac cctggtaggc ttcgatcaga gcggccgcat tctcgtctgg
     1501 ttgcgtgggc gtggcgggtg gtgcgccggg ctcaccgggc gggacgtccg gctgggccgg
     1561 cgtcgcgggt gtggctgggg tggcgggcgc ggcacccggc tgcgccggcg tggcggggac
     1621 agccggggtg gcaggcccac cctgcatcgc cgcactggcg accacttctt cgttgttact
     1681 gccaccgcac gcggcaagcg ccgctatcag ggatgttgcg aggatctttt tcattaccaa
     1741 ctccgctatg gataacaatg ctcgctccat cgcggaaacg gatacccggg atcggacgtg
     1801 tcgcaggtcc gatatgcagg cagggacaga ataagcttcc catgcaaaca actaccaaac
     1861 gctttgccga acaaaacccg atcccgctcc ggtgcctggg ccatggagcg cgaataaaac
     1921 accgatcgaa tcgatcggaa cggaccggta tgagcaaagc gcgccagacg ccgttcctgc
     1981 ccgcccggcg cggcctttga tcacacgcaa accctcaggt gttgagcgcg cgcgtcagcg
     2041 caggacgcgg catgcaaggc aggcgtgtca taaacatgaa acagtattgg acaatcaagg
     2101 ctgcgtagcc ttaatgcagc acgggaccgg ctgcgggcat gcgccgttcg gcggcgagca
     2161 tcgcttgcgc tatgcgcgcg tcgcccggga tgagcagttc gggctcgtcg gcaacaggtg
     2221 acacgctctt gacccgcatg ccctgctcat agacgagacg cccgccgaac cagcccgaga
     2281 cgatcacggc tgccgcagcg agcgcggacg ctgccagcga accgccgcgg tgtgcgccgt
     2341 cgcgttttct ggcaagcaca ttggccgccg tcatcgcgag tgcaccgctg ttgagcagcg
     2401 catgcacatt ggccgtgcgc ttgacctcgg tttcccttgg gattgccagg tagtcgagcg
     2461 ctccggccgc gcccgcggca atgccgccga cgaggccgcc catcaggctg tagtaagccg
     2521 cttccgaata ggactctttt ccggtggcac gatgcatggc atcgagcaga aagccgaaag
     2581 gaatcagtgc ggccggcgcg acgatgagca tcggatgcag cggatggccg gcgagagtgg
     2641 atttgggcat gagagtctcc tggtccaaat ccatatagtt ggcaggcgca ggccggaggt
     2701 tcccggccgg cctggtccac tcaaggcaga tcgaacacca gcacctccgc gccctcgccg
     2761 tcgcgcagcg caatggccgt ctcgccttcg gccttgagcg catcgcccgc cgcgagtgca
     2821 tgcccggcga cgccaagccg gccacgcacg agatggacat agcagcgtcg cccggcggca
     2881 agagggaggc tggcctgctc ggcgccgtcg aacaggccag cataaacgta ggcatcctga
     2941 tggatgacca ccgagccgtc gcgtccgtcc gggctggcaa tcaggcgcag gcggccgcgc
     3001 ttctcatcat cggaaaagcg cttttcctcg taacccggcg tgatgccgta gcgattcggc
     3061 tcgatccaga tttgcaggaa gtgcgtctga cggtcctgcg catggttgta ctcggagtgc
     3121 atgatgccgc gccccgcgct catgcgctgc acgtcgccgg gacggatcac gctgccgttg
     3181 ccgatgctgt cgcggtgcgc gagctcgccg tcgagcacgt agctgatgat ttccatgtcg
     3241 cgatgaccgt gggtaccgaa accggcgccc ccggccaccc ggtcttcatt gatcaccctg
     3301 agcggaccga agctcatgtg cttcggatcg tagtagtccg cgaaggaaaa gctgtgccag
     3361 gaatcgagcc agccgtggtt tgcatggccg cgttcctgtg ccttgcgcag ttcgatcatc
     3421 tcgccgccct ccatgaaaag ggcattgtcg cgcgttcgcg aaaactacgc ttgatcccga
     3481 cccttgccgg actggatcag cttgtgcagc gcgcggattt cctcgcgtaa tgcctgcacc
     3541 tcgacatgca gttcccgctc gagatgctcg ccggtctggc cgacgacgtc gcgcgtctct
     3601 tcgtgctcgg cgtcgctcac cgtctgcatc gcattgacga tgacggcgat gaacaggttg
     3661 agcatgctga aggtggcgat caggatgaac gggatgaaga acagccacgc gtacggatac
     3721 acctccatca ccgggcggga gatacccatc gaccagcttt ccagcgtcat cacctggaac
     3781 agcgtgtaga acgagcgtcc gaggtcgccg aaccagtcgg ggaagctggc ggcgaacagg
     3841 ttggttgcga tcaccgcgaa cacgtaatag atcaggagca gcaccagcgc gatcgaggac
     3901 aagccgggaa tcgctcccag cagcgcgccg accaccttgc gcatcgaggg cacgatggtc
     3961 atcacgcgca gcacccgcag cacgcgcagt gcgcgcagca ccgagagctg gccggtcgcc
     4021 ggcaccagcg cgatgccgac cacgatgaaa tcgaagatgc tccacggatc gcggaaaaag
     4081 gccagccggt acacgtagag ccggatcagg atctcgacga cgaacacggc caggatcacc
     4141 cggtccacgc cgaccagcag cgtgccgtac tcggccatca cagccggcga ggtttccagg
     4201 ccgagaatga cggcgttgat ggcgatcagc gcaaggatga tgccttgcac gcggccgttg
     4261 ttgataaagg cttccaggcg ctggcgcagg ccgcccgagg cagccgggga ggtcgtggac
     4321 gagttggcgt ttacttgcat gtctgacttc gcttatccgt tgaacaacgc gcgggaacat
     4381 gcgcgcgcac ggatttttgc acggttcccg gaagccgtca cccggccctg tcgcgccgga
     4441 cgcgcgctcc ttccgtgcgc aacagcaata caccgctggc gacgatgacg acggcaccgg
     4501 caagaacgta acggtccggc agcacttgcc aaagcatcca gtccagcatc gccgcccatg
     4561 ccagcgccga gtactcgaac ggcgcgaccg cggaagcctc gccgcggcga aacgcctcgg
     4621 tgatcagtat ctgcccgaga aagccggtaa tcgcaagcgc gatcagcagc ggccagtgag
     4681 cgcctgccag cgcgacccag ttcggcagcg ccagcagtcc tgcgcccccg gccagcgcgg
     4741 ccgtaaacca gaatacgatg ctctcgctcg aatccgtgcg tgcgagcacg cgcacggtga
     4801 tcgcgacgac cgcatagcac gcggccgagc cgagcactgt ggccgccccc aggctcagca
     4861 tgccggcgcc ggtcgggcgc aacacgatca gcaccccgac caacccgccc gctaccgccc
     4921 accagcgcgc cgcgccgatg cgttccccga ggaccagcgc ggagagcgcg gtaatcagca
     4981 gcggagcgat gaaggccagc gtgtacgctt cggccagcgg cagtgtgcgc agcgcatagg
     5041 cgaacaggaa cagcatcagg atgccggtcc cggcgcgcag cacgtgcagc ggccagcgca
     5101 cacgccgcag cgacgccgtc gcgccgcgcc accacaccca tatgcagacc aacggcaatg
     5161 cagcgaggcc gcgcaaggcg gccacctgca tggccgggta atgctgcgcg agcagcttca
     5221 ttaccgtatc catcagcgcg aacatcgcca ccgccgcgag catgaaggcg atgctgcgca
     5281 gattatcctc ccctctcaac gcagtgcggc gtcccgcgac ggacagtgac ggctcctcca
     5341 tcggcgctat cttttcagcg cgtcgcggat ctcgcgcagc agcagcacat cttccggcgg
     5401 cgcggcgggc ggcgcaggcg gcgtctgcgc ccgcaggcga ttcatctggc gcaccatcag
     5461 gaagatgacg aaggcgagga tgaggaaatt cagcgccacc gtcagaaagc tgccgtaggc
     5521 gagcaccgcc ccgcccgccc gggcctcggc aagcgttgcc gcctgctgtc cggccagcgg
     5581 aatgaagtag ttggagaagt ccagcccgcc gaccgcgagg ccgacgaccg gcatgatcag
     5641 gtccgtcacc agcgaatcga cgatgcggcc gaacgccgcg ccgatgatga cgccgacggc
     5701 aagatccatc acgttgccgc gcaacgcgaa tgtcttgaac tctgcgagca tgcccatgcc
     5761 gtcactccct gttgtgttgt gcgtcgaaac cgtcagttct gttcggcgat ctgccgcagt
     5821 gcgcgctcga acacctcggg cggctgtccg ccctggatca ggtagcgatc gttgacgatc
     5881 accgccggca cggaatggat accctgttcg agatagaagc gctgctgcgc gcgcgtctcg
     5941 tccgcatacg cgtcggagcc gagtatcgcg cgggcacgct cttcgtcgag tccgcacgcc
     6001 gccgccgtgc gcgcgagcac ctcgtggtcg ctcggatcct cgccgtcggt gaaataggcc
     6061 ttcagcagcg cgtgcttgag cgccgcctgg tgtccttcga gctgcgccca gtgcaacagg
     6121 cgatgcgcat cgaaggtgtt gtagatgcgg gagcgctttt ccatattgaa cgtgaaaccc
     6181 acctgttcgc cgcgcgccct gattgcctcc cgattctctt cgacctgcgc ttcggaaatt
     6241 ccgtacttgg caaccaggtg ctcggtgatg tcctcgcctt ccggccccat ctgcggattg
     6301 agttcgaacg gctggaaatg gatctgtgcg gcaatctctg gctgcacccg ctgcagcgcg
     6361 tgctccaggc ccgcaagccc cactgcgcac caggggcagg atacgtcgga gacgaaatcg
     6421 atcttgagtc gggtcggcat cggatcactc actttccgca tgaaggattg ccgcgaatgc
     6481 gcggatgtcc tgaactgtag acgatgcgcc gaatctatgc agaacacccg tcgaaagcga
     6541 gatgtcatgc gcgtacaagt catccacgaa gatagaaacg gcgccaagac cgacatcggc
     6601 atgttcgatc tgaatcacat gccgcccgtc ggcgagccgt ttcgtgtgaa tcccaatacc
     6661 tgcttttcct gcaaggccta cttcggcccg gacgagaaag gcatgtacct gctggtgctg
     6721 gagggtgaac cgcgcatgtt ggactagcac ccggcgcggc aggaggcgcc gccggcgttc
     6781 aagcgagtcc ccagtcgcgc tgctcgagca gcgcgatctg cgcgaagcat tcgcgctggg
     6841 cctcggacaa gtcgtgcgac acgcgcgggt cgtcctgcgc gatgcgtcct tgacggcgca
     6901 gcgtatcgag cttggccaga taatcgccgg tcaccaggat cgctcccagc gccgacatcg
     6961 gctcgcggct gcctgccccg ccgccctttt ccgctgcgtc ggcctcgccc accgcgcgga
     7021 tgacggtgtc gggaaaatgc cattcgcgcg cgatgttggc ggtgagcgcg cgtcccaagg
     7081 gaacaagggc attgcagaac gtcggcgaac cgagcggctg gctgcgatcg tagatctggt
     7141 cgatgatgcg cagcgcgacg atcaggccga cgttgtgtat caggcccgcc agaaaggcct
     7201 ccagaggatc ggcacccgcc gctcgcgcaa ggatgcggtt ggccagcgcg caacgctcgg
     7261 cctgatccca caggtgcggc gcgacccgac gcgtgaaact gcccgacttc atgtcgatga
     7321 tcggcttgaa cgccacaccg gtgatcagct ggcgcaatcc gtcctggcca aggatcagga
     7381 tcgcatggtc gatgctggtg atcggcgtgc cgggattgta ggccgcgctg ttggccagtc
     7441 gcagcacttc ggacaccagc accacatcgt gccagatcag ccgcgccagt tcctggccgg
     7501 agaagtccgg cgaacgcagg ctctgcagca gctgcgggat gacgcccggg atgcggcgca
     7561 cgagatgggt accggagcgc ggcgacttca ccgcctcgtc gatggcggcg aggatcgcgt
     7621 cctcgcaggg attggtgaac aggtccggat gattctcgga cgcgaacagc cagctggtga
     7681 actcgtagtc cgccacgctg cgctgcatcc atccgtcctc acgcgccgcg gccggcgcgt
     7741 gaggacggat ggatgcagcg cagcgtggcg g
//
LOCUS       DATLGF010000457         5726 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_77705,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000457 DATLGF010000000
VERSION     DATLGF010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77705"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VM406_14030"
     CDS             complement(<1..666)
                     /locus_tag="VM406_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HVL77129.1"
                     /translation="MKRMLFNATQQEELRVAIVDGQKLIDIDIETAGREQRKSNIYKG
                     VITRIEPSLEACFVNYGEERHGFLPFKEVARSYFKEGVDVRSASIKDALREGQEIVVQ
                     VEKEERGNKGAALTSFISLAGRYLVLMPNNPRGGGVSRRVEGEDRQELRETMDKLDLP
                     SGMSVIARTAGIGRSVEELQWDLNYLMQLWRAIEGAGKSAPGAFLIYQESSLVIRAIR
                     DYFN"
     gene            1258..2244
                     /locus_tag="VM406_14035"
     CDS             1258..2244
                     /locus_tag="VM406_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HVL77130.1"
                     /translation="MGHLIASNPSSPPSGQVRTVTISEEEADQRIDNFLLRICKGVPK
                     SHIYRVLRSGEVRVNKGRIDQTYRLRAGDIVRIPPIRVAETRAPAAPGAEFSILFEDA
                     HVLAIDKPAGVAVHGGSGVSYGVIEQLRAARPQAKFLELVHRLDRETSGILLLAKKRS
                     ALASLHQQIRDGEIDKRYLALVHGDWKNARQHVRLPLFKYTTPDGERRVRVQADGMPS
                     RTVFSLVRRLGPFALLEADLKTGRTHQIRVHLASSGFPIAGDDKYGDFALNRRLQKSE
                     GRRIAIKRMFLHAHKITFAHPDSGAPVNLHAPLPPECREFLESLAEDAQAQP"
     gene            2255..2914
                     /locus_tag="VM406_14040"
     CDS             2255..2914
                     /locus_tag="VM406_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HVL77131.1"
                     /translation="MPRKKFDLIVFDWDGTLMDSTATIVTCIQAAARDLGLPVPDKRQ
                     AAYVIGLDLNSAMQAVLPGLDPKHYPRMVERYRYHYLTRDQGLTLFDGVRDMLQELAE
                     QGYFLAVATGKSRVGLNRALDAAQLMSVFDATRCADETFSKPHPAMLHEITRELGQDI
                     ARTVMIGDTTHDLQMAINAGAAGIAVHYGAHSAPELDALRPLYSASSTAELHAWLLEN
                     A"
     gene            2907..3290
                     /locus_tag="VM406_14045"
     CDS             2907..3290
                     /locus_tag="VM406_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HVL77132.1"
                     /translation="MRDTMEERGIHVCASEALIEGGKGVRFPVTAGGEDATAFAIRYD
                     GRAYAYLNRCAHVPIELDWNEGEFFESSGLYLMCSTHGALYVPDTGRCAGGPCRGGRL
                     HSITVSEQDGQVYWQPDDYVRPARA"
     gene            3300..4331
                     /locus_tag="VM406_14050"
     CDS             3300..4331
                     /locus_tag="VM406_14050"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HVL77133.1"
                     /translation="MTTHEHDNNSATGADNPPPTRSATAASGPERKEGWERDMLERLA
                     FASLREQRAARRWRIFMRLLTLAVIAWGFWIAFGITARSTEVVARHTALIEIDGAIES
                     GARGAAESVIPALKRAFEDANSVGVILRINSPGGSPVQAGMINDEITRLRAEYPRKPL
                     YVVVDEVCASGGYYIAAAADKIFVNKASIIGSIGVMIESFGFAGAMEKLGIERRLLAS
                     GDNKGFLDPFSPQSEAHRQYAQEMLREVHQQFIDVVRSGRGDRLKETPEIFSGLFWSG
                     ARAVELGLADGFGTIDSVAREELKAEEVIDYTHREGLSELLLRRFGTSVGSSAAATLL
                     NHGWTPSLR"
     gene            complement(4578..5333)
                     /locus_tag="VM406_14055"
     CDS             complement(4578..5333)
                     /locus_tag="VM406_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HVL77134.1"
                     /translation="MTAGVLYLIPNTLGVDPGPGDPLVALIPQQVQELTAGLEYFIAE
                     NAKTARAHLKLVGARHPLARPLQEILIAELNVNTPDATLTELLAPLQAGRDAGLISEA
                     GVPAVADPGANLVRLAHREGIRVRPLVGPSSLLLAVMASGLNGQSFAFNGYLPTDAGA
                     RAKRIRQLEERSRQERQTQLFIETPYRNHALLDALLATCQSGTRLCVASDLTLESETI
                     QMHAVAHWRRLAVSGQLPDLRKRPTVFLLLASD"
     gene            complement(5337..>5726)
                     /locus_tag="VM406_14060"
     CDS             complement(5337..>5726)
                     /locus_tag="VM406_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HVL77135.1"
                     /translation="DQVATLDGVQIGKPGNHAAALQQLQRMRGRIVTFHTALCVWDGR
                     RPPAEAAQCTNVRTLVAFRDLPDSELDAYLRIEQPYDCAGSAKNEGLGIALLERIESS
                     DPTALTGLPLIALTTMLRNAGVRFFED"
BASE COUNT         1060 a         1771 c         1835 g         1060 t
ORIGIN      
        1 gttgaagtag tcacggatgg cgcggatgac gagcgaggat tcctggtaga tcaggaatgc
       61 accgggtgcg gatttgcctg cgccttcgat agcgcgccag agctgcatca ggtagttcag
      121 gtcccactgc agctcttcga cgctgcggcc gatgccggcg gtgcgcgcaa tcaccgacat
      181 gccggagggc aggtcgagct tgtccatggt ttcgcgcaat tcctggcggt cttcgccttc
      241 aacgcggcgc gaaacgccgc cgccacgggg attgttcggc atcaggacca gatagcggcc
      301 cgccagcgag atgaacgagg tcagtgccgc gcccttgttg ccgcgctctt ccttttcgac
      361 ctgaacgacg atctcctgtc cttcgcgaag tgcatccttg atgctcgcgc tgcgaacgtc
      421 gacgccttcc ttgaaatagc tgcgcgcgac ttccttgaac ggcaggaaac cgtgcctttc
      481 ctcgccgtaa ttgacgaaac aggcttccaa ggatggttcg atgcgggtga tgacgccctt
      541 gtagatattg gacttgcgct gctcgcgccc ggccgtctcg atatcgatgt cgatgagctt
      601 ctgcccatcg acgatggcca cgcgcaattc ttcctgctgc gtggcgttga acaacatgcg
      661 cttcatgatt tctccgtggc catgtggcca agcggccgcg tcggaaaacc gctgcggcca
      721 ggtacctggg atgtacgcga ggtcagagcg cttgagggag ggaatcgcct tggcgtgtcg
      781 ccggccggga cgaaaaacct ggcatggcga cggggcacgg acgagactgg ccgcgatgat
      841 gagcgaacgc cgcgaccgga tcgccgacgc cgcgagatcc gggcgcgggc ttgggctgcg
      901 ggcaacaagg ggaggccata tgatgcatgg tttcctggtt cgactatggg gccaattacg
      961 tcaaccggcg agcaatccgg aagccttgct tctcggacat gctcgccgga ataatcctta
     1021 cagatcaagc attcttgtcc aacatcgccg cgcgggtccc gatgacaact tcggtgccac
     1081 cccggacgcg cggcagtgct gcgtacacat tttctccgcc gtattcgcac tgcgcggggc
     1141 cggctgaggc gcccctgtgt agaatgtgct ttttattctc acacctgccc cggacttgta
     1201 accggagcga aacaattata tatacaaatg aaggacttag cgacaccccc gcagaagatg
     1261 gggcatttga tcgcgagcaa cccttcgtca cccccctccg gccaggtccg cacggtcacc
     1321 atcagcgaag aggaagccga tcagcgcatc gacaattttc tgctgaggat ctgcaagggt
     1381 gttccgaaga gccatatcta ccgggtgctg cgttccgggg aggttcgtgt caacaagggc
     1441 cgcatcgatc agacgtatcg gttgcgggcc ggtgacatcg tgcggattcc gccgatccgc
     1501 gtagccgaaa cgcgcgcgcc cgccgcgccg ggcgccgagt tttcgatcct gttcgaggat
     1561 gcccacgtcc tggccatcga caagccggcc ggggtggcgg tccacggggg ttccggggtc
     1621 agttacgggg tgatcgagca attgcgtgcc gcccggccgc aggcgaaatt ccttgaactg
     1681 gtgcacaggc tggatcgcga aacctctgga attctgttgc ttgcgaagaa acgttccgcg
     1741 cttgccagcc ttcaccagca gattcgcgac ggcgagatcg acaagcgcta tctcgcgctg
     1801 gtccacggcg actggaagaa cgcccggcaa catgtgcggc ttccgctgtt caagtacacc
     1861 acgcccgacg gcgaacggcg cgtgcgtgtg caggcggacg gcatgccgtc ccgcacggtc
     1921 ttcagcctcg tccggcgcct cggtccgttt gctttgctcg aagccgactt gaagaccggc
     1981 cgaacgcacc agatccgcgt gcacctggca tccagcggtt ttccgatcgc gggcgacgac
     2041 aagtacggcg atttcgcatt gaaccgccgg ctgcagaaga gcgagggcag gcgcattgca
     2101 atcaagcgga tgttcctgca cgcccacaag atcacatttg cacatccgga cagcggggcg
     2161 ccggtgaacc tgcacgcgcc gcttccaccg gaatgccggg aattcctcga aagcctcgcc
     2221 gaagacgcgc aggctcaacc ctgacagcca agccatgcca agaaaaaaat tcgatctgat
     2281 cgttttcgac tgggacggaa cgctgatgga cagcaccgcg accatcgtca cctgcatcca
     2341 ggcagccgcg cgggacctgg gcttgccggt cccggacaag cgccaggcag cctatgtgat
     2401 cgggctggac cttaacagcg cgatgcaggc ggtgctgccc ggtcttgatc ccaagcatta
     2461 cccgcggatg gtggaacgct atcgttatca ctacctgacg cgcgatcaag gcctgacctt
     2521 gttcgacggc gtgcgcgaca tgctgcagga attggctgag cagggatatt ttctcgcggt
     2581 ggcgacgggc aagagccgcg tagggctgaa cagggcgctg gacgccgcgc agctgatgtc
     2641 ggtgttcgac gccacccgat gcgccgacga gacgttctcc aagcctcatc cggccatgtt
     2701 gcatgagatc acccgggaac ttggtcagga catcgcccgc accgtcatga ttggcgatac
     2761 cacgcacgat ctgcagatgg cgatcaacgc gggggcagcc gggatcgcgg tacattacgg
     2821 cgcacacagc gcgcccgagc tggatgcgtt gcggccgttg tattcagcgt cctccacggc
     2881 ggagttgcac gcatggttgc tggaaaatgc gtgacacgat ggaggaacgc ggcattcacg
     2941 tttgcgcctc cgaagcgctg atcgagggcg gcaagggtgt gcgcttcccc gtcacagcgg
     3001 ggggtgagga tgccacggcc tttgcgatcc gttacgacgg cagggcatat gcgtatctga
     3061 accggtgcgc ccatgttcct atcgagctgg actggaacga gggcgagttt ttcgaatcga
     3121 gcggtctgta cctgatgtgc tcgacgcacg gcgccctcta cgttcccgat accgggcgtt
     3181 gcgctggcgg gccgtgccga ggcgggcgtt tgcattcaat tactgtcagc gagcaggatg
     3241 gccaggtcta ctggcagccg gacgactatg tacgtccggc gcgtgcatga aaaaacaaca
     3301 tgaccactca cgaacacgac aacaattccg cgaccggcgc cgacaatcct ccgcccaccc
     3361 ggtcggccac ggcggcatcc gggcccgagc gcaaggaagg ctgggagcgc gatatgcttg
     3421 aaaggctggc cttcgcgtcc ttgcgcgagc aacgggcggc acggcgttgg cgcattttca
     3481 tgcgcttgct caccctcgcg gtgattgctt ggggcttctg gatcgccttc ggcatcaccg
     3541 cgcgcagcac tgaggtcgtc gctcgccata ccgcgctcat cgagatcgac ggggcgatcg
     3601 aatccggcgc gcgcggcgca gcggaatcgg tgatacccgc gctcaaacgg gcattcgagg
     3661 atgccaattc ggttggcgtg atcctgcgca tcaacagccc cggcggcagc ccggtccagg
     3721 ccggcatgat caacgacgag atcacgcgct tgcgagccga gtacccgcgc aagccgcttt
     3781 acgtcgtagt ggatgaagtc tgcgcctccg gtggttacta catcgccgcg gccgccgaca
     3841 agattttcgt gaacaaggcc agcatcatcg gatcgatcgg cgtgatgatc gaaagcttcg
     3901 gttttgcggg agcgatggaa aagctcggga tcgagcgtcg cctgctggca tccggcgaca
     3961 acaagggttt cctcgacccg ttcagtccgc agagcgaggc gcaccggcag tacgcccagg
     4021 aaatgctgcg cgaagtgcac cagcaattca tcgacgtcgt gcgcagcggg cggggcgacc
     4081 ggctcaagga aacgccggag atcttctccg ggctgttctg gagcggcgct cgcgcggtgg
     4141 aactgggtct cgcggacggc ttcggcacca tcgattcggt cgcgcgcgaa gagctgaagg
     4201 ccgaggaggt gatcgactac acgcatcgcg agggcttgtc cgaactgctg ttgcggcgct
     4261 tcggaacctc ggtcggcagt tcggcggcgg cgaccctcct caatcatggc tggacaccgt
     4321 cactccgttg aagaccgatt gcccgatcct gtgaatgcaa ttcttgaatc cgtgcgcatg
     4381 ccgcctatac tggccctgtc cgattcggtt tctatccctg cattacagga cgagcatgca
     4441 gccggaaaaa ctcgcgattc ggatatccgt taccacgtct cagatgtgga catgaggatt
     4501 cggatcctct gatcgacctg cccggtgggc atgcaaaacg gcggcgcaat gccgccgttt
     4561 tgcttgtctg tcgatactca gtcggacgcc agaaggagaa acaccgtcgg gcgcttgcgc
     4621 aagtccggca attgcccgga tacggccagc ctgcgccaat gggcgaccgc atgcatttga
     4681 atcgtttcgc tctccagcgt caggtcgctc gccacgcaaa gccgtgtgcc ggattgacag
     4741 gtggcgagca acgcgtccag cagtgcgtgg ttgcggtaag gcgtttcgat gaaaagctgc
     4801 gtctgtcttt cctggcgcga gcgctcctcg agttggcgga tgcgtttggc gcgcgccccg
     4861 gcgtcggtgg gcagataacc gttgaatgcg aaactttgcc cattcaggcc ggaagccatc
     4921 accgccagca gcagagacga cggccccacc agcggccgta cccggatgcc ttcgcgatgc
     4981 gcgagccgga ccaggttggc gccggggtcg gcaactgccg gcactcccgc ctccgaaatc
     5041 agcccggcgt cccgccccgc ctgcaagggt gcaagcagct ccgtcagcgt cgcgtcgggc
     5101 gtgttgacgt tcagctcggc gatcaggatt tcctggagcg gcctggcaag cggatgtcgc
     5161 gcccccacca gcttcaggtg agcacgggcc gtcttcgcat tctcggcaat gaaatactcc
     5221 aggcccgccg tcagctcctg cacctgctgc ggaatcaacg cgacgagcgg atcgcctggg
     5281 cccgggtcga cgcccagcgt gttcgggatc aggtagagga cgccagccgt catcggtcaa
     5341 tcctcgaaga agcgcacgcc ggcattgcgc agcatggtag tcaaagcgat cagcggcaga
     5401 ccggtcagtg cggtcgggtc gctgctctcg atacgttcga gcaaggcaat gccaagaccc
     5461 tcgttcttcg cactgccggc gcagtcgtat ggctgctcga tgcgcaggta ggcgtcgagc
     5521 tcactgtcgg gcaggtcgcg aaacgcgacc agggtccgca cgttcgtgca ttgggcggcc
     5581 tcggcgggcg ggcgccggcc gtcccagacg cacaacgcgg tatggaaggt cacgatgcgc
     5641 ccgcgcatgc gttgcaattg ctgcaacgcg gcggcgtggt tgccgggttt gccgatctgg
     5701 acgccatcca gcgttgcgac ctggtc
//
LOCUS       DATLGF010000458        13251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_173978, whole genome shotgun sequence.
ACCESSION   DATLGF010000458 DATLGF010000000
VERSION     DATLGF010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 13251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13251
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173978"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="VM406_14065"
     CDS             <1..1209
                     /locus_tag="VM406_14065"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVL77136.1"
                     /translation="EDSMDGKYVVLRTRPSRTGDPFARAFAFPRATEVQPADFSIEVD
                     DLNKKELADQLRDPEVQAVAPSMPMRLIAPRPYSLGDEAEPASQGVAWGVQAVGADTS
                     PYSGAGVVVAVLDTGIDASHPAFSGMDLVQKDFTGEGNGDDNGHGTHCAGTIFGREVQ
                     GQRIGVAPGVSKALIGKVLGRDGGGSSEEICNAIFWAMDSGAHVIAMSLGMDFPGYQK
                     RLQARGLPQEVATSWALDGYRKNVQLFERLAALVRARSAFSQGSLLVAAAGNESNRTQ
                     GEYWEVAVSPPAVSEGFVSVGALRRTAQGLAVAPFSNTGANICGPGVDIVSASPGGGL
                     RSLSGTSMATPHVAGVAAHWAQKLRNSGGLVSLHLISRLIGSGTTMGLRAEFDPLDIG
                     AGLARCPQQE"
     gene            1266..1667
                     /locus_tag="VM406_14070"
     CDS             1266..1667
                     /locus_tag="VM406_14070"
                     /inference="COORDINATES: protein motif:HMM:NF019199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HVL77137.1"
                     /translation="MIPLKRFVRKRCAGRLHRRFFSMERKLVIQLRRVIMKQLSIEMP
                     IVAECAVRECVYNTEQCCHARAITVGNGVHPGCDTFFNGSAHSKAWSRTAGVGACKVS
                     ACKFNEDYECMTEQISVGRDGESVRCLTYQR"
     gene            1843..2211
                     /locus_tag="VM406_14075"
     CDS             1843..2211
                     /locus_tag="VM406_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77138.1"
                     /translation="MSPFNRLPGSRRAAAGLEWKVLKKLPATLLSAALGCACFILLLR
                     AGWFDLGDKEALTAQYSTLGFLLSFAIITLHVAFVCGIIIVMKGHAYVWDAYYLPDQD
                     SPAAPLRARVVQKGRERGQR"
     gene            complement(2375..3004)
                     /locus_tag="VM406_14080"
     CDS             complement(2375..3004)
                     /locus_tag="VM406_14080"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL77139.1"
                     /translation="MPARPDELARISTITLQHYQAHAAPFWEGTRDHDVRQNLDALLR
                     HIQGSPPFRILDFGCGPGRDLAALRALGHEPVGLDGCAAFASMAREHSGCEVLQQDFL
                     ALDLPASRFDGIFANASLFHVPSRELPRVLRELRATLKPRGVLFASNPHGNNEEGWSG
                     GRYGAYYDLAAWRGFMRAAGFVELEHYYRPEGMPREQQRWLASVWRSAS"
     gene            complement(3112..3603)
                     /locus_tag="VM406_14085"
     CDS             complement(3112..3603)
                     /locus_tag="VM406_14085"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HVL77140.1"
                     /translation="MNESKGAFPADKPVDALIHDHNTVRKLVDVYRNSDSMAAKISAA
                     EQALMLLESHSLVEEQVFYPAVRDVDASMISHFEEEHHKTDDILASLKRMSLSDPQAL
                     PMFEQLVDMVLHHIEEEENQFFPKLEQARMDMTPIGLQMQAYEANLVHMQAQASDQQG
                     ARR"
     gene            3693..4049
                     /locus_tag="VM406_14090"
     CDS             3693..4049
                     /locus_tag="VM406_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77141.1"
                     /translation="MTAIMLLRRDAFPTPAVAGRTALRAFCLSRTIPAIIDEANMETV
                     TYQRGHWKAEAHAEELASGKYQGIVLLFHEGGESSEQILRRTLNDSDTPEEALEEAKV
                     LARTILDRESAPGARG"
     gene            4327..4614
                     /locus_tag="VM406_14095"
     CDS             4327..4614
                     /locus_tag="VM406_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77142.1"
                     /translation="MSDKTTRENIVEAADELFYRLGFEHTSFSGDDGVFLLVGSLQPG
                     LGGAIVAHATSLPELEARVNADPFVMENVVTTRILQVAPAKADQRLRFLLG"
     gene            4797..5906
                     /locus_tag="VM406_14100"
     CDS             4797..5906
                     /locus_tag="VM406_14100"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HVL77143.1"
                     /translation="MTNPIRRIAINTGGGDAPGLNAVIRAVVLAALKRGWECHGIRDG
                     FNGILYPERYPEGGVIRLTQDRVRGIAHLGGTILGTTNKGDPLHFPVRMPDGSVTVMD
                     RTDAILHYFEQAGLDALVSVGGDGTLSIAHALHRKGLRVVSVPKTIDNDLDKTFTTFG
                     FDTAVQFAADCIDRLHSTAESHQRIMVVEVMGRYAGWIALHAGIAGSAHGILIPEIPF
                     DIDKVVGKIESRERLGRMYSIVVVAEGAHARGGERAVLAPAEAGRVERLGGIGEQVAA
                     ALQARTGKDARVVVLGHLLRGGSPTAFDRLAALRFGAAAVRALDEGKTGIMVSLAFPN
                     VEYVPIEEVAGRMKAVPPDSDTLQTGRDLGICFGD"
     gene            6216..7208
                     /locus_tag="VM406_14105"
     CDS             6216..7208
                     /locus_tag="VM406_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77144.1"
                     /translation="MKPAVVAASVLFLSFSAAPVAKADARHVISQPKKLDAMVKDKRR
                     PVIHVKRGGWGGAREEDIEHLLAAIATDLLQHFPGRELGPISVSPSVHGPVVLYQKGP
                     RNEYQILLSARGENWAEYVYEFSHELLHVLANYEHHAHDTRHLWFEEMLGEAVSLHML
                     KRFSNAWRESPPLPQHVSYAGDLSRFTQRALFAESRRLPRGVTFEQWFRVNGPILSAN
                     PHAREKNDLVAGLFLPLLEHMPDWTAFAHLNAGQQQAAPSFHEHLLRWYWNTPAEHRQ
                     MVVEAMHLFHFTPPDDLAGNAIPAAASAPAAPARPLGSEPHLHRHVQQGPQARH"
     gene            7818..8177
                     /locus_tag="VM406_14110"
     CDS             7818..8177
                     /locus_tag="VM406_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77145.1"
                     /translation="MNTRSLIATVAAFATLATGGIAYAAMPNGEIPESNYVGSNLSRG
                     QVMSDLESARARGITGTDVDTIDGGAATHAPRMAGAHGPAGSRFADRTREEVRAEREE
                     YWREHGRLPGHDPLYIN"
     gene            complement(8237..8920)
                     /locus_tag="VM406_14115"
     CDS             complement(8237..8920)
                     /locus_tag="VM406_14115"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HVL77146.1"
                     /translation="MKHDEQHSEMRRRTMLALLGGAFVGSGVAPPGARAAAPACVLAP
                     RQPEGPFFVDTRLQRSDIRSGTSGVPLTLRLRVVDADRDCAAVHGLALEVWHCDPRGL
                     YSGVRDPRAGLVEGDFLRGYQITDAQGIVQFTTVYPGWYRGRAVHVHIKLRDRQREWS
                     SQLYFDDRITDQVHAREPYRAGGGRRTRNEDDGLYRHGGERLMLALAPEGGGYAAQFE
                     LGVIGLGRV"
     gene            9177..9605
                     /locus_tag="VM406_14120"
     CDS             9177..9605
                     /locus_tag="VM406_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVL77147.1"
                     /translation="MLHMEMHLGRLIDHIQLIVADLGASRRFYEAIFAALEIPPSGSG
                     DDYFWYDELYVSTPDSPAAQGRLTGRHHLAFQARNRDMVDAFYKAGLAAGGRDNGAPG
                     ERSYHPGYYAAFLLDPDGNNIEAVYHGQAARSAPSIKITF"
     gene            complement(9646..10056)
                     /locus_tag="VM406_14125"
     CDS             complement(9646..10056)
                     /locus_tag="VM406_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77148.1"
                     /translation="MSDLRNDYDDRPDSPSLRPWIPLQSTYDVEAWIDSYNRELQRAA
                     GKANAAGYGICFQLSHGGELYMHTTPEGEVLLDVTHEAQWVAPLIAAAAQVPEPAGRI
                     WSIPADRMTQLIFGLSSLIDASRIVLAHDFRRKK"
     gene            10284..10739
                     /locus_tag="VM406_14130"
     CDS             10284..10739
                     /locus_tag="VM406_14130"
                     /inference="COORDINATES: protein motif:HMM:NF025701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HVL77149.1"
                     /translation="MKTGAIARLCRTLPFVPLLVSCGGISIGIFFSTLDRSVHSGVDT
                     FRAVVVSEPTAWATLWSAHTSRLTPAPPPPLVDFSTRTVVAVFLGPRPNACYAVRIEE
                     IRTAPDRIVVYFTERRPGLHDVCAQVITTPAHLVIVERTGLPFEFIKTN"
     gene            complement(10779..11351)
                     /locus_tag="VM406_14135"
     CDS             complement(10779..11351)
                     /locus_tag="VM406_14135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HVL77150.1"
                     /translation="MTDSVHRVALIIGSTREGRFGPTVARWLHSQVRARGDMEADLID
                     LADAGLEQAFARAEPSAVASLGARLHAADAFLIVTPEYNHGYPAPLKTAIDWYQTQWH
                     AKPVGFVSYGGISGGLRAVEQLRQVFAELHATTVRDAVSFPNAWEQFDERGELLEQER
                     YARRASVMLERLLWWSEALRAARRKRAYPA"
     gene            complement(11344..12132)
                     /locus_tag="VM406_14140"
     CDS             complement(11344..12132)
                     /locus_tag="VM406_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77151.1"
                     /translation="MNEESVKHPFIPNTPAAARVGPVLPLLPQFGRQDAAAVLISEWR
                     VDSAARQRATLGAISVTWNARPWPAGLHDYAVFPSVDGRTLLHYSQWTDGAAFDGFVQ
                     HQRDTRVDQIDAAVPGIERYGLGRFRFYRSHVESAAGAATGCLVAVRVEADSAERQRA
                     WIDTVIEALASARIPGLIAAHFHVCDDGRIVNLAQWESEDAHIRAVEGNGGMAQREGS
                     AWQRVRTMPGVTQKGVQRFHLPLLVGRTLAAERAANDSQGGKHD"
     gene            12240..12680
                     /gene="soxR"
                     /locus_tag="VM406_14145"
     CDS             12240..12680
                     /gene="soxR"
                     /locus_tag="VM406_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HVL77152.1"
                     /translation="MNTLMTISEVARRSGVPASALRFYEQRGLIESERAGSGHRRYPR
                     AVLRRLAFIVFAQRIGLSLDEIGTELGKLPGNATPRSEDWAALSGTWAARIEQRIEEL
                     ERLKSGLTECIGCGCLSLDHCRLVNPEDRAARFGPGPVRWSGQE"
     gene            12811..>13251
                     /locus_tag="VM406_14150"
     CDS             12811..>13251
                     /locus_tag="VM406_14150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HVL77153.1"
                     /translation="MQAVRIHQYGGRERLSIDDIAVPAVAPDEVLVRVVAASVNPVDW
                     KIRAGHLAQMIAHPLPLTLGWDVSGVVEAVGEAVTEWKVGDAVYSRPDLKRHGTYAEF
                     VAIRANECARKPRTISHVEAASLPLAGITAWEVLMSTAQVRAGQP"
BASE COUNT         2272 a         4292 c         4386 g         2301 t
ORIGIN      
        1 gaggacagca tggacgggaa atatgtcgta ctgagaacac ggccgagcag gaccggcgat
       61 ccgttcgcac gggcatttgc gtttccgcgc gccaccgagg tgcagccggc cgatttttcg
      121 atcgaggtcg acgacctgaa caagaaggag ctcgcggacc agctgcgcga ccccgaggtg
      181 caggcggtcg ccccgtcgat gccgatgagg ctgatcgcgc cgcggcccta cagcctgggg
      241 gacgaagccg agccggccag tcagggcgtt gcatggggcg tacaggcggt cggcgctgat
      301 acctcgccat attccggcgc aggggttgtg gtcgctgtgc tcgataccgg catcgatgca
      361 agccatccgg cgttcagcgg catggatctg gtgcagaagg atttcaccgg cgaggggaat
      421 ggcgacgaca acgggcacgg cacccactgc gccggcacga tcttcggtcg agaggtgcag
      481 ggccaacgca tcggcgtggc gccgggcgtg agcaaggcat tgatcggcaa ggtgctcggc
      541 cgcgacggcg gcggatcgag cgaagagatc tgcaatgcga tcttctgggc gatggacagc
      601 ggcgcccacg tgatcgcgat gtcgctcgga atggactttc cgggctacca gaagcgcctg
      661 caggcgcgcg gccttccgca ggaagtggcg acctcatggg ccctggacgg ttatcgcaag
      721 aacgtgcagc tgttcgagcg ccttgcggcg ctggtcaggg cgcgcagcgc gttctctcag
      781 ggatcgctgc ttgttgccgc ggccggcaac gagagcaacc gcacgcaggg cgagtactgg
      841 gaggtggccg tcagcccgcc tgcagtatcg gagggcttcg tgtcggtcgg cgcgctgcga
      901 cgtaccgcgc agggtctcgc ggtggcgccg ttctcgaata cgggcgcaaa catctgcggt
      961 ccgggcgtgg acatcgtttc ggcctctccc ggcggcgggc tgcgttcact cagcggtacc
     1021 agcatggcca caccgcatgt cgccggcgtg gctgcgcatt gggcgcagaa gctgaggaac
     1081 agcggcggcc tggtttcgct gcatctgatc tcgcgcctga tcggctcggg cacgacgatg
     1141 ggcctgcgcg ccgagttcga tccgctggac atcggcgccg gtctggcgcg ctgcccgcag
     1201 caggaatagg tacaggcgcc cggagcttgc cgtgatgctc cggagcatgg tcaggcgcag
     1261 ccgtgatgat tccgctcaag cgcttcgtgc gcaagcgctg cgcgggccgc ctgcatcgac
     1321 gattcttttc gatggagcgc aagctcgtca ttcagcttcg aagggtcatc atgaaacagc
     1381 tatcgataga aatgccgatc gtggcggagt gcgcggtacg cgaatgcgtg tacaacaccg
     1441 agcagtgctg tcatgcgcgc gccatcacgg tcggaaacgg cgtgcatccc ggctgcgata
     1501 cgttcttcaa cggctccgcg cattcgaagg cgtggtcgcg cacggccggc gtcggcgcct
     1561 gcaaggtatc cgcctgcaag ttcaacgagg actatgaatg catgaccgaa cagatcagcg
     1621 tcggccgcga cggcgagtcc gtccgatgtc ttacctatca gcgctagcgg gtgctcgggc
     1681 gtgatgatgc ggcaggccaa gtccccgctt tcgaacggag cgagggcatc atgagttcgc
     1741 gtgccgcagg gtatctgttc ccggttcaag actttctgcc gttgcgcggc actcctgttc
     1801 cgttgcgttg tcctagcaga aagggcaaac ccaggagcat gaatgagtcc gttcaatcgc
     1861 ctgccgggat cgcgccgcgc cgctgccggg ctcgagtgga aggtgctgaa gaagcttccg
     1921 gccacgctgc tgtccgccgc tctcggatgc gcgtgcttca tcctgctgct gcgcgccggc
     1981 tggttcgacc tcggcgacaa ggaggcgctg acggcgcagt actcgacgct cgggtttctg
     2041 ctgtcgttcg cgatcatcac gctgcatgtt gcattcgtct gcggaatcat catcgtgatg
     2101 aaggggcatg cctacgtatg ggatgcctac tacctgccgg atcaagacag tccggcggcg
     2161 ccgttgcgtg cgcgggtggt gcaaaagggc cgggaacgcg gtcagagata gcagctgcgc
     2221 cggacgaacg cggcatgcgc tgccgcgcgg gagatcgttg tccttgaagc gcgtatcttg
     2281 cgagggcggt cttgggggtg gtggcccgca agggcaaccc gggccgacga agtgcatgcg
     2341 gtggcgcaag aagggcagcg ggcgcgccgc gtggtcacga tgcgctgcgc cagacgcttg
     2401 ccagccagcg ctgctgctcg cgcggcatgc cttccggtcg gtagtagtgc tccagttcga
     2461 caaagccggc cgcccgcatg aaaccccgcc aggcggcgag atcgtaatag gcgccgtagc
     2521 gcccgccgct ccatccttcc tcgttgttgc cgtgtggatt ggatgcgaac agcacgccgc
     2581 gaggtttgag tgtcgcgcgc agctccctca gcacgcgtgg caattcccgg ctcggcacat
     2641 ggaacaggga agcgttcgca aagatgccgt cgaagcgcga tgcggggagg tcgagcgcga
     2701 ggaagtcctg ctgcaagacc tcgcagccgc tgtgttcgcg ggccatcgag gcgaacgccg
     2761 cgcagccgtc caggccgacc ggctcatggc ccagcgcgcg caaggccgcg agatcccgcc
     2821 cgggaccgca gccgaagtcg agaatgcgaa acggcggaga gccctggata tggcgcagca
     2881 gcgcatccag gttctgcctg acgtcatggt cgcgcgtgcc ttcccagaaa ggcgcggcgt
     2941 gcgcctggta atgctgcagc gtgatcgtgc tgatgcgggc gagttcgtct ggtcgggctg
     3001 gcatcggacc gttcattcgg gaagcggaaa agggcgcgcc caggcggcgc gactgcaatg
     3061 atagggcagg gccgcctgct ttgcctgttg tcgacgccct tcggagcgcc cctaacgccg
     3121 cgccccctgc tgatcgctgg cctgcgcctg catgtggacg aggttcgctt cgtatgcctg
     3181 catctgcagc ccgatcgggg tcatgtccat ccgagcctgt tcgagcttcg ggaagaactg
     3241 attctcctct tcctcgatat ggtgcagcac catgtcgacc agttgctcga acatcggcag
     3301 cgcctgcggg tcggagagcg acatgcgctt gagcgaggct aggatgtcgt cggtcttgtg
     3361 gtgttcctcc tcgaagtggc tgatcatcga ggcgtcgacg tcgcgcaccg cgggatagaa
     3421 cacctgctct tccaccagcg agtgcgattc cagcagcatc agcgcctgct cggctgcgct
     3481 gattttggcc gccatgctgt cgctgttgcg atagacatcg acaagtttgc gcacggtgtt
     3541 gtggtcgtgt atcagtgcgt ccacgggttt atccgccgga aaggcgccct tgctttcatt
     3601 catgtccgta ctcctgttcc atctgcgatt ggccgcgcaa tgcgttttcg aggggacgcc
     3661 tttggcgaag ttcaaggcgc gccgacgccg gaatgacggc gatcatgctt ctgcgacgcg
     3721 atgcgttccc cacgcccgcc gtggcgggca gaaccgctct gcgcgcgttc tgtctcagcc
     3781 gaaccattcc cgcaatcatc gatgaggcga acatggaaac cgtcacctat caacgggggc
     3841 actggaaggc cgaggcgcac gcagaagaac tggccagcgg caagtaccag ggcatcgtcc
     3901 tgctgttcca cgaaggcgga gagtcgtccg agcagatact gcgccgcacg ctgaacgact
     3961 cggacacgcc ggaagaggcg ttggaagaag cgaaggtgct ggcgcgaacg atactggacc
     4021 gcgagtccgc gccgggcgca cgcggctgac ctccctgaca ccgctcagcc tgccgtcacc
     4081 agccttgccc gaacgtgtgc ggcaggcccg ttgccaccgc gccgggggtg acggggcgcg
     4141 ccgccgcgcg ttcacgcacg cgttcggctc gcgcgctgcc ggctcgccgg ccgccttgac
     4201 ttgcccttga ctacttagga cgcccgtcct agtccgtgcg cgccccgagc gacggctggc
     4261 gatgtacgcg gctcgcttga gcggtttcag ttgtggtgac ccggcacggc cgggcaggtg
     4321 aacaggatga gcgacaagac cacgcgcgaa aacattgtcg aggcggccga cgagctgttt
     4381 tatcggttgg gtttcgagca cacctcgttc tcgggtgacg acggtgtatt tctgctggtt
     4441 ggctcgctgc aaccgggttt gggcggggcg atcgtcgcac acgcaacgtc gctgcccgaa
     4501 ctcgaagcca gggtgaacgc tgatccgttc gtgatggaga acgtcgtgac aacccggatt
     4561 ctgcaggttg cgcccgcaaa ggccgaccag cgcctgcggt tcctgctcgg ctgacgcggg
     4621 aggggcgtaa cgcgcactgg cagttttcca ccgggtgaag ccgggccgcg gttgaagcgc
     4681 cggggttgcc atggcaagat gcaaatccct gcgcgctccc gagcaggcac gaggttgccg
     4741 gcagtaagca cggccgcgcc cggcgccatc ccgacccgac tctctcccag gatcccgtga
     4801 ccaatccgat ccgacgcatc gcaatcaaca ccggcggcgg cgacgccccc ggcctgaacg
     4861 ccgtcatccg cgccgtcgtg ctcgccgcgc tgaagcgggg atgggaatgc catggcatcc
     4921 gcgatggctt caacggcatt ctctatcccg aacgctatcc ggaaggtggc gtgatccgtc
     4981 tcacccaaga tcgggtgcgc ggcatcgccc atctcggcgg cacgatcctg gggacgacga
     5041 acaagggcga tccgctgcat tttcccgtgc ggatgccgga tggctcggtc acggtgatgg
     5101 accgcaccga tgcgatcctc cactacttcg aacaggcagg gctggatgcg ctcgtgtcgg
     5161 tcggcggcga cggcacgctc tcgattgcac acgcgctgca tcgcaagggc ctgcgcgtgg
     5221 tcagcgtacc caagaccatc gacaacgacc tcgacaagac cttcaccacc ttcggtttcg
     5281 acaccgccgt gcagttcgcc gccgactgca tcgaccggct gcacagcact gccgagagcc
     5341 atcaacggat catggtggtg gaggtcatgg gccgctacgc cggatggatc gccctgcatg
     5401 ccggcatagc cggcagcgcg cacggcattc tgattcccga gatcccgttc gatatcgaca
     5461 aggtggtcgg caagatcgaa agccgcgagc ggctcggccg catgtattcg atcgtcgtcg
     5521 tcgccgaagg cgcgcacgct cgtggcggcg agcgggcagt gctcgcgccg gccgaggccg
     5581 gccgggtcga gaggctcggc ggcatcggcg aacaggtggc agcggccttg caggcgcgca
     5641 ccggcaagga cgcgcgggtg gtcgtgctcg gtcacctgct gcgcggcggc agccccaccg
     5701 ccttcgaccg cctggcggcc ctgcgcttcg gcgccgccgc cgtacgtgcg ctggacgaag
     5761 gcaaaaccgg catcatggtg tcgctcgcct ttccgaatgt ggagtacgtt ccgatcgagg
     5821 aagtcgccgg gcgcatgaag gctgtaccgc ccgacagcga cacgctgcag acggggcgcg
     5881 acctggggat ctgcttcggg gactgaggac cggtgtccgt gcgtggaaat atcggtcggc
     5941 aatttcttta tacgtatccg gatttgcgca tgaggtcgcc atgtcccgtg catcgacgca
     6001 cccgcgcaac atctcgcaca tgtcatgctc aaacgccctg tgacgccgaa ccttctggct
     6061 cgctccgctt ctaatcaaag aaaacctgag tccgcgacgg cgcaaatcct gccgccgcgg
     6121 ttcccgccct tccgactcgc ccgcgaagtt ggcgaacggc accggccgga cacggcccga
     6181 ttcacgtctt accttggatc ctttcgagaa ctaccatgaa acccgcagtc gtggccgctt
     6241 cggtcctatt tctctcgttc agcgccgcgc ccgtggcaaa ggcggacgcc cggcacgtca
     6301 tcagccagcc caagaagctc gacgccatgg tgaaggacaa acgccggccg gtgattcacg
     6361 tcaagcgcgg ggggtggggt ggagcaaggg aggaagacat cgagcatctc ctcgcagcca
     6421 tcgcaacaga cctgttgcag cattttcctg gccgggagct gggtccgatt tccgtttcgc
     6481 cgtccgtgca tggtcccgtt gtgctgtacc agaaggggcc gcggaacgaa tatcagatcc
     6541 tgctctcggc aagaggcgag aactgggccg agtatgtgta cgagttctcg cacgagctgt
     6601 tgcacgtgct cgcaaactat gagcatcacg cgcacgatac gcgccatctg tggttcgagg
     6661 agatgctcgg tgaagcggta tcgctgcata tgctgaaacg gttttccaac gcatggcggg
     6721 agtctcctcc gctgccgcaa catgtgtcct atgccgggga tctgagccgt ttcacgcagc
     6781 gggcgctgtt cgcggaaagc aggcgcctgc cccgcggcgt cacgttcgaa caatggtttc
     6841 gcgtgaacgg tccgatactc agcgcaaatc cgcacgcgcg cgaaaaaaac gaccttgtcg
     6901 ccggtctctt cttgccgctg ctcgaacaca tgccggactg gacggcattt gcacacctga
     6961 acgccggtca gcagcaggcg gcgcccagct tccacgaaca cctgctgcgc tggtactgga
     7021 acacgcccgc cgagcatcgg caaatggtcg tcgaggcgat gcacctgttc catttcacgc
     7081 caccggacga cttggccggg aacgcgatac ccgccgccgc cagtgcgcct gctgcccctg
     7141 cacggccgct cggcagcgaa ccccatctcc accgccacgt tcaacagggc ccgcaggcgc
     7201 ggcattaagg ctacgctgga caagtcctcg tgcgtcgcgc atcccgaact cgggcggtct
     7261 gcggcgttgc gaccctcgca gggcgcgctc cggcttgcaa gccgggactg ctacgcaggc
     7321 gggcagcgtg ctgcccgaaa cccctgcttc gccctcgcga tagccccgct atcgctgcgg
     7381 ctcgcgcctt gcatcccctc ccgatttcgg gcacgcgcca tcacgggact tattcagcct
     7441 agccttaaat cgacggtgcg caatgccctt gcgcgcgcca gtttgtgctt gcagtcgcat
     7501 gggcgcgaca tatactgacc aagcactgac cagtgcaatt gcatcatcgg cggtccgccc
     7561 ttgagacgaa tctgtgcgca taccccgcac cggtggatct gcaccacaaa tgcgtgaccc
     7621 tgcctcatcc gccttccgct gacgcacgtc agtgtgaccc gtaagcgaaa gctcgcatac
     7681 ggcttttttg gaagcgcaag tcgcaccacc cggactgtcg gcaagaagcg gcacaacaac
     7741 cggccacgga acagtactga agtcttgtac cagggaggag ctggacaagt tgcgaccttc
     7801 ttggcaagga catcatcatg aacactcgct cacttatcgc gacggtcgcc gcattcgcga
     7861 ccctcgcaac cggtggcatc gcctatgctg cgatgccgaa tggagaaatt cccgagtcca
     7921 actacgtcgg ctccaacctg tcgcgcggcc aggtcatgag tgacctcgaa agcgcgcgcg
     7981 cccggggcat caccggtacc gacgtcgaca cgatcgatgg cggggccgct acgcatgcac
     8041 cgcgcatggc tggcgcgcac ggcccggccg gatcccgctt cgcggaccgc acacgtgaag
     8101 aggtgagggc ggaacgggaa gaatactgga gggagcacgg tcgcctgcct ggccatgacc
     8161 cgctgtatat caattgaccg atgaacatcg taaccgggcg tcgcctgcat aaacggcggt
     8221 gcggcgcccg cgtccctcac accctgccca acccgatcac acccagttcg aactgcgctg
     8281 cataaccgcc cccctcgggg gccagcgcga gcatcagcct ttcgccgcca tgccggtaca
     8341 ggccgtcatc ctcgttgcgg gtgcgccggc cgccgccggc acgatatggt tcccgtgcat
     8401 gcacctggtc ggtgatgcgg tcgtcgaaat acagctgcga actccattca cgctgccggt
     8461 ctcgcagctt gatgtgaacg tgcaccgccc gtccccgata ccagccggga tagacggtgg
     8521 tgaactgtac gatcccttgc gcgtccgtga tctggtagcc gcgcaggaaa tcgccctcga
     8581 ccaatccggc gcgcggatcg cgcacgccgg agtacaggcc gcgcgggtcg caatgccaga
     8641 cctccagcgc gagtccatga acggccgcgc agtccctgtc ggcgtccacc acgcgcagcc
     8701 gcagggtcaa gggtacgccg ctcgtgccgg accggatgtc ggagcgctgc agccgcgtgt
     8761 cgacgaagaa tgggccctcc ggctggcgcg gcgccagcac acaggccggc gcggcggcgc
     8821 gcgcaccggg aggggcaaca ccgctgccga cgaatgcacc gcctagcagt gcaagcatcg
     8881 tgcggcggcg catttcacta tgctgttcgt cgtgcttcat cttcgctgct cctcgcgggg
     8941 tgcccggacc tgtgcagtat atgccgcaga cgcccccggc agtaacgacg gcaagacctg
     9001 cttcaccttg tgcgacgtgc gcaaaagcgg tgcagactag tgcatgcggc gtccacgtcg
     9061 gcgcatgcgt atcacgcggt tccgccaggc gacgcatggt cgttcaggcg ctcgaacaac
     9121 gtcgctcgaa caacgtcgct cggaaccttc aatggacttg cctgaacgaa aggaggttgc
     9181 tgcacatgga aatgcatctt ggccgcctga tcgatcacat ccagctgatc gtagcggatc
     9241 tgggggcgag ccgccgcttc tacgaagcga tcttcgcggc actcgagatt ccgcccagcg
     9301 gttccggcga tgattatttc tggtacgacg agctgtatgt ctccaccccg gacagtccgg
     9361 ccgcgcaggg aaggctgacc ggccgccatc atctcgcctt tcaggcgcgc aatcgggaca
     9421 tggtcgacgc gttctacaag gcaggacttg cggcaggcgg cagggacaac ggcgccccgg
     9481 gagagcgctc gtatcacccc ggctactatg cggccttcct gctcgacccg gacggcaaca
     9541 atatcgaggc ggtctaccat ggccaagccg cgcgcagcgc gccatcgata aagatcacgt
     9601 tctgaacgcg gcgcgggtcg cgccggtcgg gccgcaatgg cgatgttatt tcttcctgcg
     9661 aaagtcgtgc gcgagcacga tgcggctcgc atcgatcagg ctggagagac caaagatcag
     9721 ctgtgtcatc cggtcggcgg ggatgctcca gatgcggccc gccggctcag gtacctgcgc
     9781 cgccgccgca atcagcggcg ctacccactg cgcctcatgc gtcacgtcga gcagcacctc
     9841 gccctccggc gtcgtgtgca tgtacagttc gccgccatgc gacagctgga agcagatgcc
     9901 atagccggct gcgttcgcct ttccggccgc gcgctgcagt tcgcggttgt agctgtcgat
     9961 ccaggcttcg acgtcatatg tcgattgcag tggtatccac gggcgcaggg aaggggagtc
    10021 gggacggtcg tcgtaatcgt tgcggaggtc ggacatcgtt cgggaaagag ggataggcct
    10081 ggcgcgacac taaccgacaa tcgccaacca tgccagcgaa aacgcggccg cggagcgggc
    10141 cggcttcggc cggtgtctgg cgggcatgtg cggacccgaa gacgaaagtc tgacgcagat
    10201 cagtgtccgc gtttgcggcg gcgcaatgct gtcgtttcga ggcgctgttg cgccaggcgc
    10261 gggcgccgaa caaggagaga cgcatgaaga ccggggcgat cgcacggctt tgccgcacgc
    10321 tgccgtttgt tccgttgctg gtgtcgtgcg gcggcatttc gatcggcatt tttttctcga
    10381 cgctggacag gtccgtgcat tcgggcgtgg acactttccg ggccgtggtg gtgagcgaac
    10441 cgacggcgtg ggcgacgctt tggtcagcgc acacctcgcg cctgacgccc gcgccgccgc
    10501 cgccgctggt ggacttttcc accaggacgg tcgtggccgt attcctcggt ccccggccca
    10561 acgcctgcta tgcggtgcgc atcgaggaga tccgcaccgc gcccgatcgc atcgtcgtct
    10621 acttcaccga gagacgccca ggcctgcacg acgtctgcgc gcaggtcatc actacacccg
    10681 ctcacctcgt gatcgtcgag cggaccggtc ttccgttcga attcatcaag accaactgac
    10741 cgatttcggc ttgcacgtgc ccggcgcgca agcccgggtc aagcgggata ggcgcgcttc
    10801 ctgcgcgccg cgcgcagtgc ctcgctccac cacagcaggc gctccagcat gacggacgcg
    10861 cgccgcgcat accgctcctg ctccagcaac tcgccgcgtt cgtcgaactg ttcccaggcg
    10921 ttgggaaagc tgaccgcgtc gcgcaccgtc gtcgcgtgaa gttcggcgaa tacctggcgc
    10981 agttgctcga ccgcgcgcag gccaccggaa atgccgccgt aggagacaaa accgaccggc
    11041 ttggcatgcc attgggtctg ataccagtcg atcgccgttt tcagcggcgc cggatagcca
    11101 tggttgtact cgggagtgac gatcaggaat gcatcggcag catgcaggcg tgcgcccagg
    11161 ctcgccacgg ccgatggctc ggccctggca aacgcttgct ccagaccggc atcggcgagg
    11221 tcgatcaggt ccgcctccat gtcgccacgc gcgcgaacct ggctgtgcag ccagcgcgcg
    11281 acggtcggcc cgaagcggcc ctcccgggtg ctgccgatga tgagtgcgac gcgatgaacg
    11341 gaatcagtca tgcttgcctc cctgcgagtc attggccgca cgttccgccg caagggtccg
    11401 gccgacgagc agcggcaggt gaaagcgttg cacgcctttt tgcgtgacgc ccggcatcgt
    11461 gcgcacgcgc tgccaggcgc tgccctcccg ctgtgccatg ccgccgtttc cttccactgc
    11521 gcggatgtgc gcgtcctcgc tctcccattg cgcgagattg acgatgcggc cgtcatcgca
    11581 gacatggaaa tgcgcggcga tcagtccggg tatgcgcgcg ctcgcgagcg cctcgatcac
    11641 ggtgtcgatc caggcgcgct gccgctcggc ggaatcggct tcgacgcgca cggcgaccag
    11701 gcatccggtg gcggcaccgg cggcggattc gacgtggctg cggtagaaac ggaagcgtcc
    11761 caggccgtat cgctcgatac ccggcaccgc cgcgtcaatc tggtcgaccc gggtgtcacg
    11821 ctgatgctgc acgaagccgt cgaacgccgc gccgtctgtc cactgcgagt agtgcagcag
    11881 ggtgcggcca tcgaccgagg ggaagaccgc gtagtcatgc aggccggccg gccacggccg
    11941 cgcgttccag gtcacgctga tcgcgcccag cgtcgcccgt tggcgcgcgg cggaatcgac
    12001 ccgccactcg ctgatcagga cagcggcggc atcctggcgg ccaaactgag gcagcagcgg
    12061 caagaccggg ccgacgcggg cggcagcggg agtgttcgga atgaatggat gcttcacgga
    12121 ttcttcgttc atcggcatgg ctccttgtgt gcggttgagg ccggcaggca ccggcacgac
    12181 acaggctaca agttaaagtg aacttgaagt caagggccat gtgcactatg cttttgccga
    12241 tgaacacact gatgacgatt tccgaagtgg cgcgccgcag cggcgtgccc gcctccgcgt
    12301 tgcgcttcta cgaacagcgc ggcctgatcg aatccgaacg cgccgggtcc ggccaccggc
    12361 gctacccgcg cgcggtcctg cggcggctcg cgttcatcgt gttcgcgcaa cgcatcggtc
    12421 tgtcgctcga cgagatcggg accgagctgg gaaaactgcc cggcaatgcg acacccaggt
    12481 ccgaggactg ggcagcgttg tccggcacgt gggcagcacg catcgagcaa cgcatcgagg
    12541 agctcgagcg attgaaatcg ggcctgaccg agtgcatagg gtgcggttgc ctgtcgctcg
    12601 atcattgccg gctggtcaat ccggaagacc gggcggcgcg ctttgggcct ggcccggtgc
    12661 gctggtccgg gcaggagtga cgtcgctgct ggccatgtca cggctgtcgc acggaaacga
    12721 gtgcgcaggc cctcccgtac cggctgcggc atgccgcgcc gccgaacctt cagccggcgc
    12781 attcttctaa ccccggaaca aggagtgagc atgcaggcag tacgcattca tcagtacggg
    12841 ggacgcgagc gcctttccat cgacgacatt gcggttcccg cggtcgcgcc ggacgaagtg
    12901 ctggtgcgcg tggtggcggc gtcggtcaac ccggtcgact ggaaaatccg cgcgggccat
    12961 ctggcgcaga tgatcgcgca tccgttgccg ctgacgctgg gctgggatgt gtcgggcgtg
    13021 gtcgaggcgg tcggcgaagc ggtgaccgaa tggaaagtcg gcgatgcggt ttattcgcgc
    13081 cccgatctca agcgccatgg gacctatgcg gagttcgttg cgatacgggc gaacgaatgc
    13141 gcgcgcaagc cgcgcaccat atcgcacgtc gaggcggctt cgctgccgct tgcaggcatc
    13201 acggcgtggg aagtcctgat gagcacggcg caggtgcggg ccggccagcc c
//
LOCUS       DATLGF010000459         3389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_12723,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000459 DATLGF010000000
VERSION     DATLGF010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12723"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VM406_14155"
     CDS             <1..426
                     /locus_tag="VM406_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVL77154.1"
                     /translation="PWASRAAIDAEELKSETMLLLGNGHCFRDQVLEVCPEMSRFSTS
                     GDGIARTFEGSSLETIRHMVASGIGITVLPVASVPDMQAQDGLLRYIPFAEPAPARRV
                     VIASRKSFTRGAAIAAVRDAVLACRLNGVTMLEEEAADV"
     gene            419..1306
                     /gene="ubiA"
                     /locus_tag="VM406_14160"
     CDS             419..1306
                     /gene="ubiA"
                     /locus_tag="VM406_14160"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HVL77155.1"
                     /translation="MSELAARSPSRFQLYLRLVRLDKPIGILLLLWPTLIALWLASGG
                     LPEWHLIAIFTLGTVLMRSAGCAINDYADRDFDRHVKRTADRPLTSGQISPREALIVA
                     SALAITAFLLILPLNLLTKQLSVAAVLIAASYPYFKRFFAIPQAYLGIAFGFGIPMAF
                     AAVQDTVPPLAWLLLLANIFWAVAYDTEYAMVDRDDDLKIGIKTSAITFGRFDVAAVM
                     FCYAAALAIVLAVGWQFGLRTWFFAGMAVAAAFALYHYTLIRERDRMRCFAAFRHNNW
                     LGAAIFIGVALELALRPAV"
     gene            1374..1691
                     /locus_tag="VM406_14165"
     CDS             1374..1691
                     /locus_tag="VM406_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HVL77156.1"
                     /translation="MSATGNGSGREGLLDDLRLVIKDAEDLLRSTGSRVDGGYQAARA
                     RFESTMSDARESLSGMEEQLVEGAREALDQADKYVRENPWQAIGIGAAVGLIAGLLLG
                     RRD"
     gene            1695..1988
                     /locus_tag="VM406_14170"
     CDS             1695..1988
                     /locus_tag="VM406_14170"
                     /inference="COORDINATES: protein motif:HMM:NF025363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK family protein"
                     /protein_id="HVL77157.1"
                     /translation="MPETPQRKKARLLAQSAALRIELARSMRPLGYQLRSAESGARSL
                     ARLQFHPEWIAGAMMVIALLRPERISSWVRGMTHGLRTWRRFAPLLEGVLRRG"
     gene            complement(2028..2852)
                     /gene="proC"
                     /locus_tag="VM406_14175"
     CDS             complement(2028..2852)
                     /gene="proC"
                     /locus_tag="VM406_14175"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HVL77158.1"
                     /translation="MQNEVKIGFVGGGNMAAALIGGLAGKLTSGGNIHVVDLNAEALQ
                     RLHARFGVTTSQDIGPELAGCDVIVLAVKPQQMKDVVARVAQHVKSQLVLSIAAGIRA
                     ADLSRWMNGCRAIVRTMPNTPALIGKGITGMVAMDGVSAAQRDLADAIMRAVGETVWL
                     DDDAMIDPVTAISGSGPAYVFYFIEAMQQAAQEMGLSAEQGNRLAIATFVGAAELAAQ
                     SDEPVSVLRERVTSRGGTTYAALTSMEASGVKAALVDAMKAAAARGKELGEEFGKD"
     gene            complement(2842..>3389)
                     /locus_tag="VM406_14180"
     CDS             complement(2842..>3389)
                     /locus_tag="VM406_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HVL77159.1"
                     /translation="FGENYVQEGLDKIDAVRALLPADVLEWHFIGPLQSNKTRAVAAH
                     FDWVHSVERVRIAQRLSDQRPAHLPPLNVCLQVNISGEASKSGVAPGEVETLARAIAA
                     LPKLRLRGLMAIPEPAEDVPAQRAPLHAMKLLFERLRGQGLPLDTLSMGMSQDLEAAV
                     AEGATIVRIGTAIFGKRHYAE"
BASE COUNT          529 a         1172 c         1089 g          599 t
ORIGIN      
        1 ccctgggcgt ctcgcgctgc tatcgacgcc gaggaactga agtccgagac catgctcctg
       61 ctcggcaacg ggcactgctt ccgagaccag gtgctggaag tctgcccgga gatgtcgcgc
      121 ttttcgacct ccggcgacgg catcgcacgc accttcgagg gctcgtcgct ggaaacgata
      181 cgccacatgg tcgcatcggg catcggcatt accgtgctgc cggtcgcctc ggtgccggac
      241 atgcaggcgc aggacggctt gctgcgctac atcccgttcg ccgaaccggc gcccgcgcgc
      301 cgggtggtga tcgcgtcgcg caagagcttc acccgcggcg cggcgatcgc ggcggtacgc
      361 gacgcggtgc tggcatgccg gctcaatggc gtcacgatgc tggaagagga ggcggccgat
      421 gtctgaactc gccgcccgtt ccccgagccg cttccagctc tacctgcggc tggtgcgcct
      481 ggacaagccg atcggcatcc tgctgctgct gtggccgacg ctgatcgcgc tctggcttgc
      541 gtcgggcggt ctgcccgaat ggcatctgat cgcgatcttc acgctcggca ccgtgctgat
      601 gcgctcggcc ggctgcgcga tcaacgacta cgccgaccgc gatttcgacc gccatgtgaa
      661 gcgcacggcc gatcgtccgc tgaccagcgg gcagatctcg ccgcgcgagg ccctgatcgt
      721 tgcgtcggcc ctggcgatca ccgcctttct gctgatcctg ccgctgaatc tgctgaccaa
      781 gcagctgtcg gtcgcggcgg tgctgatcgc ggcgagttat ccgtatttca agcgcttctt
      841 cgcgattccc caggcctacc tcggcatcgc gttcggattc ggcattccga tggcgttcgc
      901 cgcggtccag gatacggtgc cgccgctggc ctggctgctg ctgctggcca atatcttctg
      961 ggcagtcgcc tatgacaccg agtacgcgat ggtcgaccgc gacgacgacc tgaagatcgg
     1021 catcaagacc tcggcaatca ccttcggccg tttcgacgtc gcggccgtga tgttctgcta
     1081 cgcggctgcg ctggcgatcg tgttggcggt cggctggcag ttcggcctgc gcacctggtt
     1141 cttcgccggc atggcggtgg cggcggcgtt cgcgctgtac cactacacgc tgatccgcga
     1201 acgcgaccgc atgcgctgct ttgcggcctt ccgccataac aactggctgg gggcggcaat
     1261 cttcatcggc gttgcgctgg agctggcgct gcggccggct gtttgacagg tcgcaagata
     1321 gcgagacccc ccttccctac actcagggaa acacccactt catggaggca ggaatgtctg
     1381 cgacaggcaa tggcagcggc cgcgaaggtt tgctggacga tttgagactc gtgatcaagg
     1441 acgcggaaga cttgttgcgt agcaccggct cgcgcgtgga cggcggctac caggccgcgc
     1501 gcgcccgctt cgagtccacg atgagcgatg cacgcgaaag cctttccgga atggaggagc
     1561 aactggtcga aggcgcgcgc gaagcgctcg accaggcgga caagtacgtg cgcgagaatc
     1621 cctggcaggc gatcggcatt ggcgcggccg tgggcctgat cgccgggctg ctgctcggtc
     1681 ggcgcgactg aggaatgccc gagacgccgc aacggaagaa ggcgcggctg ctcgcgcaaa
     1741 gcgccgcgct gcgcattgag ctggcgcgca gcatgcgccc gctcggctac caactgcgct
     1801 cggccgaaag cggcgcccgt tcgctcgcaa ggctgcagtt tcacccggaa tggatcgccg
     1861 gagcgatgat ggtgattgca ctcctccgcc ccgagcgcat ctcgtcctgg gtgcgcggaa
     1921 tgacccacgg gctgagaaca tggcggcgct tcgcgccgct gctggagggc gtgttgcggc
     1981 gggggtgaga cacataagca ccggcgcccc cgcttgtatg tgaacggcta atccttcccg
     2041 aactcctcgc ccagctcctt gccgcgcgcc gccgctgcct tcatcgcatc gaccagtgcg
     2101 gccttcacgc cactggcttc catgctggtc aacgcggcgt aggtcgtgcc gccgcgcgaa
     2161 gtcacgcgct cgcgcagaac cgataccggc tcgtccgact gcgcggcgag ttccgctgcc
     2221 ccgacgaagg tcgcgatcgc cagccggttg ccctgctcgg cggacaggcc catctcctgg
     2281 gcggcctgct gcatcgcctc gatgaaatag aacacatagg ccggaccgct gccggaaatc
     2341 gccgtcaccg ggtcgatcat cgcatcgtcg tccagccaca cggtctcgcc caccgcgcgc
     2401 atgatcgcgt cggccaggtc acgctgcgca gcgctgacgc cgtccatcgc gaccatgccg
     2461 gtaatgccct tgccgatcag ggccggcgtg ttgggcatcg tgcgcacgat cgcccggcag
     2521 ccgttcatcc agcgcgacag gtcggccgcg cgaatgccag ccgcgatcga cagtaccagc
     2581 tggctcttca catgctgcgc gacgcgcgcg accacgtcct tcatctgctg cggcttcacc
     2641 gcaagcacga tgacgtcgca gccggccagt tccggaccga tgtcctgcga ggtcgtgacg
     2701 ccgaagcgcg catgcaggcg ctgcagcgcc tcggcattca aatcgaccac gtggatgtta
     2761 ccccccgagg tcagcttgcc ggccagcccg ccgatcaggg cggcggccat gttgccgccg
     2821 ccgacgaaac cgatcttcac ttcattctgc atagtgtctc tttccaaaaa tcgcggttcc
     2881 aatccgcacg atggttgcgc cttcggccac cgccgcctcc aggtcctgtg acatgcccat
     2941 cgacagggta tcgagcggca gtccctgccc gcgcaggcgt tcgaacaaca gcttcatcgc
     3001 gtgcagcggc gcgcgctggg ccggaacatc ttccgccggt tcgggaatcg ccatcaagcc
     3061 gcgcaggcgc agcttcggca gcgcagcaat cgcgcgcgcc agcgtctcca cctcgccggg
     3121 cgccaccccg ctcttgctgg cctcgccgct gatattgacc tgcaggcaca cgttcagcgg
     3181 cggcaggtgt gcgggccgct ggtcggacag gcgttgcgca atgcgcacac gttcgactga
     3241 gtgcacccag tcgaaatgcg cggccacggc tctcgtcttg ttgctctgca gcgggccgat
     3301 gaaatgccat tccagcacgt ccgccggcag cagcgcacgc actgcgtcga tcttgtcaag
     3361 cccctcctgc acatagttct cgccgaaag
//
LOCUS       DATLGF010000460         7475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_12766,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000460 DATLGF010000000
VERSION     DATLGF010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 7475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7475
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12766"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VM406_14185"
     CDS             <1..858
                     /locus_tag="VM406_14185"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HVL77160.1"
                     /translation="VHALSLAFSMSSGGSLQFGTEAKPLHCGMAAEWGIWAAQLAQQG
                     FEGHPNVFQGKGSFQAMYAGGDPAEPFQPELYPAEPLAIDAYPPVAKLYPCCGSSHLG
                     IDALRALRARQTFDVADVIRVDVHMLKIMCDTLRYQMPTNEKEARFSMPYCAAVTLVH
                     GMPTLAHFSAQAIATPHPQVARLLPLVHTHVRTPTPAVLAKQLIFQGDCLVEVRLRNG
                     EVLREVWEHPKGCADNPLTADERWGKFVECASVRLGEEQATRLYEKLLALFNLPSIAE
                     LAPIARMPH"
     gene            complement(965..1867)
                     /locus_tag="VM406_14190"
     CDS             complement(965..1867)
                     /locus_tag="VM406_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVL77161.1"
                     /translation="MDLRQLETFLSVVDAGSFSRAAAALNLAQPSVSRQIALLEADLG
                     QRLLTRTGRGVVPTEAGNALVVHARVMLDAAKRAREEMREMHASPAGRVIVGLPPRVA
                     LGLSESLIQRFRERFPRAVITVLEGLSIPLRESLIAGRLDLALLFDPPASPQLNYHQL
                     MRERLFLVGPPGIRLPARVELSTLGNYPMLLPSAPNAIRSLLDAVLRPHRIELQLLAE
                     VGAVNTVLGLVAKGLGCTVLPESAVTSSNGPDLPRAPISSPAIWNTLVLAIPNARPAT
                     RLTRETAQLLRELDFRGPQYVRSR"
     gene            1939..2778
                     /locus_tag="VM406_14195"
     CDS             1939..2778
                     /locus_tag="VM406_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVL77162.1"
                     /translation="MNLTLPNGACNAHCHVFGPRGKFPYAPDAAFIPADDAPKEALFE
                     LNDRLGLQRCVVVQSTCHGFDNSAAEDAVAARPASYRAIALLPTDVSDRELKRLDAAG
                     FRGVRFNFMGHLGSRAPIDEVLALAARLEPLGWHLQIHGDPSLLTDLAPALLRSPVPV
                     VIDHIGRIDAGLGLQQDAFQALLALMRDERFWVKVSGMDRITRTGPPYEDAQPFARTL
                     ISEFGDRVVWGNDWPHPNHAGPVPDEQQLVDLIAQIAPEPAQRERLLVANPQRLYRFE
                     DKT"
     gene            2775..3575
                     /locus_tag="VM406_14200"
     CDS             2775..3575
                     /locus_tag="VM406_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVL77163.1"
                     /translation="MKARFENKVAIVTGAGCVGEGWGNGRAIAVRLAEEGAKVLAVDR
                     DPARLDETLALAGDARESIKPWICDVTSSDSVAAMAAACVEAFGTIDILVNNVGGSAP
                     GGPVELSEDVWDLQVDFNLKSVFLTCKHVLPTMLAKKSGSIVNISSTSGLRWTGSAQV
                     AYAATKAGVIHLSRVVAVQHAAQGVRVNCVVPGQLHTPMVEARLAKQRTGGDVDTLLA
                     QRQKRIPLGFMGDGRDTASAVLFLASDEARFITGTELVVDGGMTARCD"
     gene            3618..4580
                     /locus_tag="VM406_14205"
     CDS             3618..4580
                     /locus_tag="VM406_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVL77164.1"
                     /translation="MNKTRRLITIAALCLAASGGAIAQSKPVRIIVSFTPGGPVDTVA
                     RILADQLGKEIGRTVIVENKPGANGAIGANEVLRSRPDGDTLWITSVGAAAINASLYE
                     RLSYDMQRDFAPVSLVVNNEEVLVINPNDQAKDAAEFVKAAKARKEPTPMASSGIGSI
                     PHLAIEQLADATSADFLHVPYKGAAPALTDVMGGQVAGFFGDIPGLIAHVRGGRLKAV
                     GIASSKRHPALPDVKTLEEQGIKGVDTNNWYGLFAPAKTPPATIEALNKATHRALMNP
                     GVRERLQGLGTDSAPSTPQELAAMLKTDTEKWARLIRAKNIKAE"
     gene            4580..5107
                     /locus_tag="VM406_14210"
     CDS             4580..5107
                     /locus_tag="VM406_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HVL77165.1"
                     /translation="MAASDIRVPLVQPGTRPELAELEARIMAERGRISLLYQVLLNSA
                     PIATGWERMLTAVRNQTGVPADLRELMILRVAVLNGAGFEFDAHIPHAERAGVSKEKI
                     AATRQVPLQGSFSPLEQLVLELTDAMTRDVVVPDALMQRLREHFDAKGIVEVVATVAA
                     YNMVSRLLVALNVEH"
     gene            5113..5667
                     /locus_tag="VM406_14215"
     CDS             5113..5667
                     /locus_tag="VM406_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77166.1"
                     /translation="MKQDLWLLKLAGQDQAVAERVQELSRQVPRLDAWRAPQESLSYL
                     YLPAQAEGDIPLPRAEGDDWRRLRCTLVIEGAAAAQPVRCHYVVETDVLPECEDEFNA
                     WYNEEHLPGLAAVPGTVYAARYVDTAGSPRYYACYGLTDASVMGSPAWLAVRGTAWSS
                     RVRPTFRHTRRTMFEPFFPASAAA"
     gene            6085..>7475
                     /locus_tag="VM406_14220"
     CDS             6085..>7475
                     /locus_tag="VM406_14220"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HVL77167.1"
                     /translation="MSVISPATQQNAPSTVRFTDFGLSPDILRALEDQGYEYPTPIQA
                     QAIPVVLQGRDVMGAAQTGTGKTAGFSLPIIQLLLKHANTSMSPARHPVRALILTPTR
                     ELADQVADNVKAYSRHTPLRATVVFGGVDMAPQTAALRGGVEIVIATPGRLLDHVQQK
                     TVNLGQTEIFVMDEADRMLDMGFLPDLQRIINLLPKERQNLMFSATFSPEIKKLANSF
                     LKSPVLIEVARSNATAENVTQILYKVDEEDAKRDAVAHIIRERQLKQVIVFSNTKIGA
                     SRLSRHLEKEGINASAIHGDKTQQERMAALEAFKQGGIEVLVATDVAARGLDIAELPC
                     VINFDLPYNAEDYVHRIGRTGRAGASGDAISLYTDKDARLLADIEKLTKQSIKRLELT
                     GFAPARGGERRRREGGREGGREERSQEARGSQYGRSAYGARKEKVDPWFLKPYEPASS
                     STTSTEEDGKQVPP"
BASE COUNT         1374 a         2459 c         2454 g         1188 t
ORIGIN      
        1 gttcatgcgc tgagtctggc attcagcatg tccagcggcg gcagcctgca gttcggcacc
       61 gaggccaaac cgctgcactg cggcatggcg gcggaatggg ggatctgggc ggcgcagctt
      121 gcccagcagg gattcgaagg ccatccgaat gtatttcaag gcaagggctc gttccaggca
      181 atgtacgccg gtggcgaccc ggccgagccg ttccagcctg aactctatcc ggcagagccg
      241 ctggcgattg acgcgtaccc gccggttgca aagctgtacc cgtgctgcgg ctcgtcgcat
      301 ctcggcatcg atgcgcttcg cgcgctgcgc gcgcgccaga cattcgacgt cgccgacgtc
      361 attcgcgtgg acgtccatat gctgaagatc atgtgcgaca cgctgcgcta tcagatgccg
      421 acgaacgaga aggaggcgcg cttcagcatg ccttattgcg ccgcggtgac gctggtgcac
      481 ggcatgccga cgctcgccca cttctcggcg caggcaattg cgaccccgca cccgcaagtc
      541 gccaggctcc ttcctctcgt ccatacacac gtgcgcacgc ccaccccggc ggtgctggcc
      601 aagcagctga tcttccaggg cgactgcctg gttgaagtgc gcctgaggaa cggggaggtc
      661 ctccgcgagg tctgggagca tccgaaaggg tgtgcggaca acccgctgac ggcggacgag
      721 aggtggggca agttcgtcga gtgcgcgagt gtccggctgg gtgaggagca ggcgacgcgg
      781 ctctacgaaa agctgctagc gttgttcaat ctgccttcca ttgccgagct tgcccctatc
      841 gcaagaatgc cccattagat tccctagatc gcctaagcac aggccgaaca ggactcgctc
      901 gtccgccatg tgtacgcgac gaattgcaaa ccaaccggta gctggaggtc gtcatcggcg
      961 tgcgtcagcg cgacctgaca tactgcggcc cgcgaaagtc cagctcacga agcagttgcg
     1021 cggtctcgcg ggtcagacgc gttgccgggc gcgcgttggg tatcgccagc acgagcgtgt
     1081 tccagatggc cggcgagctg atcggcgcgc gcggcaggtc ggggccgttg ctgctcgtga
     1141 ccgcgctttc cggcaggacg gtgcagccaa gccctttggc cacgagtccc agcacggtgt
     1201 tgaccgcgcc cacctcggcg agtagctgca gttcgatacg atgcggtcgc aacaccgcgt
     1261 cgagcaagct gcggatggcg ttaggggcgc tgggcagcag catcggatag ttcccgagcg
     1321 tgctcagctc gacgcgggca ggaagcctga tccccggtgg gccaacgagg aacagccgct
     1381 cgcgcatcaa ctggtggtag ttcagctgag gcgaagccgg cggatcgaac agaagtgcga
     1441 ggtcaagccg gccggcgatc agtgactcgc gcaagggaat gctcagtccc tcgagaaccg
     1501 taatcaccgc acgcgggaag cgctcccgga agcgctggat aagagactcg ctcaaaccca
     1561 gcgccacccg cggcggcagc ccgacgatca cgcgccccgc cgggcttgca tgcatctcgc
     1621 gcatttcctc tctggcgcgc ttggccgcgt cgagcatcac gcgtgcatgc acgacaagtg
     1681 cgttgcccgc ttcggtggga acgacgccgc gcccggtacg cgtcagcagc cgttggccga
     1741 ggtccgcttc caatagcgcg atctggcggc tgacactggg ttgcgcaagg ttgagcgcgg
     1801 ccgcggcacg gctgaagctt cccgcatcca ccacgcttaa gaaggtttca agctgtcgca
     1861 ggtccatagc gaacacgcat aggtgatata gaaggtatgt ccttgtttgg gggagtgtac
     1921 accgtcacac tccctgtcat gaacctcaca cttcccaacg gcgcgtgcaa cgcgcattgc
     1981 cacgttttcg gtccgcgcgg caagtttccg tatgcgcccg atgcggcctt cattcccgcc
     2041 gacgacgccc ccaaggaggc gttgttcgaa ctgaacgacc gcctcggcct tcagcgctgc
     2101 gtggtcgtgc agtcaacctg ccacggtttc gacaacagcg cagccgaaga tgcggtggcg
     2161 gcgcgtcccg ccagctaccg cgcgatcgcg ctgctgccga ccgacgtgtc cgatcgcgag
     2221 ctcaagcgcc tcgatgccgc cggctttcgc ggcgtgcgat tcaacttcat ggggcatctc
     2281 ggcagccggg cgccgatcga cgaggtgctg gccctggctg cacggctcga accgctgggc
     2341 tggcacctgc agatccatgg cgatcccagc ctgctgacag accttgcgcc ggccctgctg
     2401 cgttcgccgg ttccggtggt gatcgaccat atcggtcgca tcgatgccgg actcggcctg
     2461 cagcaggatg ccttccaggc gctgcttgcg ctgatgaggg acgagcgctt ctgggtcaag
     2521 gtcagcggca tggatcgcat cacgcgcacc ggcccgccgt acgaggacgc gcagccattt
     2581 gccagaaccc tcatcagcga gttcggcgac cgcgtggtgt ggggcaacga ctggccgcac
     2641 ccgaatcacg ccgggcccgt gcccgacgag cagcagctgg tcgacctgat tgcgcagatt
     2701 gcaccggagc cggcgcagcg cgagcggctt ctggttgcca atccccaacg actttaccga
     2761 tttgaagaca agacatgaaa gcgcgatttg aaaacaaggt agcgatcgtg accggcgcag
     2821 gatgcgtcgg cgaaggctgg ggcaacggac gggcgatcgc cgtgcggctc gccgaagaag
     2881 gcgccaaggt gctggcggtg gaccgcgatc ccgcacgcct ggacgaaacg ctcgcgctgg
     2941 ccggcgatgc gcgcgaatcg atcaagccct ggatctgcga cgtcaccagc agcgacagcg
     3001 tggcggccat ggcggcggct tgcgtcgagg ccttcggcac gatcgacatt ctggtcaaca
     3061 acgtcggtgg gtcggcgccc ggcgggccgg tggaattgag cgaggacgtc tgggacctgc
     3121 aggtcgactt caacctgaag agcgtcttcc tgacctgcaa gcacgtgctc ccgacgatgc
     3181 tggccaagaa gtccggttcc atcgtcaata tatcgtcgac atccggcctg cgctggaccg
     3241 ggagcgccca agtcgcgtat gcggcaacca aggcgggggt gattcatctg tcgcgcgtcg
     3301 tcgcggtcca gcatgcggcg caaggggtgc gcgtcaactg cgtcgtaccg ggtcagttgc
     3361 ataccccgat ggtcgaggcc cgcctggcca agcagcgcac cggcggggat gtggacacgc
     3421 tgctggcgca gcgccagaag cgcattccgc tcggcttcat gggcgacgga cgcgacaccg
     3481 ccagcgccgt gcttttcctg gccagcgacg aagcgcgctt cataaccgga accgagttgg
     3541 tggtcgacgg cggcatgacc gcacgctgcg actgacgcga cgaaacacaa ggcatacata
     3601 caaccaagga gatgagcatg aacaagacca gacgcctgat cacgattgcc gcgctgtgcc
     3661 tggctgcatc cggcggcgca atcgcccagt ccaagccggt gcgcatcatc gtgtcgttca
     3721 caccgggcgg cccggtcgac acggttgcgc gcatcctcgc cgatcagctc ggcaaggaga
     3781 tcggccggac cgtgatcgtc gaaaacaagc cgggcgcgaa cggcgcgatc ggcgccaatg
     3841 aggtgttgcg gtcccgtccg gacggcgaca ccttgtggat caccagcgtc ggcgctgcgg
     3901 cgatcaatgc ctcgctgtac gagcgtctca gctacgacat gcagcgggac tttgcgccgg
     3961 tctcactcgt cgtcaacaac gaggaagtac tggtgatcaa ccccaacgac caggcgaagg
     4021 acgccgccga gttcgtcaag gcggcaaagg cgcggaagga accgacgccg atggcttcgt
     4081 cgggcatcgg cagcattccg catctcgcta tcgagcagct tgcggacgcc accagcgcgg
     4141 acttcctgca cgtcccctac aagggtgcgg cgcctgcgct caccgatgtg atgggcggcc
     4201 aggttgccgg cttcttcggc gacatccccg gcctgatcgc gcatgtgcgc ggcgggcggt
     4261 tgaaggcggt cggcatcgct tcctccaagc gtcatcccgc tctgcccgac gtgaagacgc
     4321 tcgaagagca gggcatcaag ggcgtggata cgaacaactg gtatggcctg ttcgccccgg
     4381 cgaagacacc gcccgcaacc atcgaggcgc tcaacaaggc gacgcatcgt gccctgatga
     4441 acccgggcgt gcgcgaaagg ctgcagggcc tgggaaccga ctccgccccg tcgacgccgc
     4501 aggaactggc ggcgatgctc aagaccgata ccgaaaaatg ggccaggctg atccgggcca
     4561 agaacatcaa ggcggaatga tggcggcatc cgacatccgc gtgccgctgg tgcaacccgg
     4621 cacgcgcccc gaactggccg agctggaggc tcgcatcatg gcagagcgcg ggcgcatctc
     4681 gctgctctac caggtcctgc tgaacagcgc accgatcgca acaggctggg aacgcatgct
     4741 caccgccgtg cgcaaccaga ccggcgtacc ggccgacctg cgcgagctga tgatcctgcg
     4801 cgttgccgtg ctcaacggcg ccgggttcga attcgacgcg cacattccgc atgccgagcg
     4861 tgccggcgtc agcaaggaga agatcgcggc gacacggcag gtaccgcttc agggcagctt
     4921 cagcccgctg gagcagctag tcctggaact gaccgacgcc atgacccgcg acgtggtcgt
     4981 gcccgatgcg ctgatgcagc ggttgcgcga gcacttcgat gcgaagggca tcgtggaagt
     5041 ggtggcgacg gttgccgcct acaacatggt gtcgcggctg ctggtggcat tgaacgtgga
     5101 gcactgaacg gcatgaaaca ggatctctgg ctgctgaagc tcgccgggca ggaccaggct
     5161 gtcgcggaac gcgtgcagga gctgagccgg caggttccgc ggctcgacgc gtggcgcgcg
     5221 ccacaggaga gtctgagcta cctctacctc ccggctcagg ccgaaggcga catcccgctg
     5281 ccgcgcgccg aaggcgatga ctggcgtcgc cttcgctgta ccctcgtgat cgagggtgcg
     5341 gccgccgcgc agccggtgcg ctgccactac gtggtggaaa cggacgtgtt gcccgagtgc
     5401 gaagacgagt tcaacgcctg gtacaacgaa gagcacctgc cggggctggc ggcggtgcca
     5461 ggaaccgtgt atgcggcgcg ctatgtcgac acagccggct cgccgcgcta ttacgcgtgc
     5521 tatgggctga ccgacgcctc cgtcatgggt agcccggcat ggcttgcggt gcggggcacc
     5581 gcatggagct cccgtgtacg gccgacattc cgtcatacca gacggacgat gttcgaacca
     5641 ttcttcccgg cctcagccgc cgcgtagaga gcggcgcgaa ctcgcccctg atgcacggga
     5701 cggggcgcca tcgcggcgca gatccgctct ggatggcgtt acgaccgacg cgctggctct
     5761 ccagcgcgcc tcgcttctcg gaaacggccg tgtggcgccc ctgggcgcgg cggcacttct
     5821 caccgcacct tcccccagcc ggcgcgccta tacgagggcg ctagcacatc ttcacccgaa
     5881 acggaactcc cgcgcgcgac aggcgtataa tgcgcggttg ttctatcacc gatgcggcgg
     5941 ccctcgcgat ccctgcgaca caggcgggga aatatccaac gaggctggag ctgccgcgcc
     6001 gggcgctagc gtccggacgc agagcgcatc gcgctttcct ccatgtaaat cctggccgcg
     6061 aagcggccga ccgaatcgag cacaatgtcc gttatttctc cagcaacaca gcagaacgcc
     6121 ccatccaccg tccgtttcac cgatttcggc ctgtcccctg acatcctgcg cgcgctcgag
     6181 gaccagggct acgaataccc gacgcctatc caggcgcagg cgatccccgt ggtgctgcaa
     6241 ggccgcgacg tcatgggcgc ggcgcagacc ggtaccggca agacagccgg cttctcgctg
     6301 ccgatcatcc agctgctgct gaagcatgcc aacaccagca tgtcgccggc gcggcacccg
     6361 gtgcgggcgc tgatcctcac cccgacccgc gagctggccg accaggtggc ggacaacgtc
     6421 aaggcctact cgcgccacac gccgctgcgc gcgaccgtcg tcttcggcgg cgtggacatg
     6481 gcgccgcaga ccgccgcgct gcgcggcggc gtcgaaatcg tgattgccac ccccggacgg
     6541 ctgctcgacc atgtccagca gaagacggtg aacctcggac agaccgagat cttcgtgatg
     6601 gacgaagccg accgcatgct cgacatgggc ttcctgccgg atctgcagcg catcatcaac
     6661 ctgctgccga aggagcgcca gaacctgatg ttctcggcca ccttctctcc ggaaatcaag
     6721 aaactggcca acagcttcct gaagagcccc gtgctgatcg aagtggcgcg cagcaatgcc
     6781 acggcggaaa acgtcaccca gatcctgtac aaggttgacg aggaagacgc caagcgcgac
     6841 gcagttgcgc acatcatccg cgagcgccag ctcaagcagg tgatcgtgtt ctcgaacacc
     6901 aagatcggcg catcccgtct ttcgcgccac ctggaaaagg aaggcatcaa cgcctccgcg
     6961 atccatggcg acaagacgca gcaggagcgc atggccgcgc tggaagcctt caagcaaggc
     7021 gggatcgaag tgctggtcgc caccgacgtg gcggcgcgtg gtctggatat cgccgagttg
     7081 ccctgcgtga tcaacttcga cctgccgtac aacgccgaag actatgtgca ccgcatcggc
     7141 cgcaccggcc gcgccggtgc gtccggcgac gcgatttccc tctacacgga caaggatgca
     7201 cgcctgcttg ccgacatcga gaagctgacc aagcagagca tcaagcggct cgaactcacc
     7261 ggctttgcgc ctgcgcgagg cggtgagcgg cggcgtcgcg agggcggccg cgagggcggt
     7321 cgcgaggagc gcagccagga agcgcgcggt tcgcagtacg ggcgcagcgc ctatggtgcg
     7381 cgcaaggaaa aggtcgatcc gtggttcctg aagccgtacg agccggcgtc gtcgagcacc
     7441 acgtcgactg aagaggatgg caagcaggtc ccgcc
//
LOCUS       DATLGF010000461         3992 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_154810, whole genome shotgun sequence.
ACCESSION   DATLGF010000461 DATLGF010000000
VERSION     DATLGF010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154810"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="VM406_14225"
     CDS             <1..1080
                     /locus_tag="VM406_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HVL77168.1"
                     /translation="IALSAVLSGVKDIREAVERAELVRATSGRRTILFVDEVHRFNKS
                     QQDAFLPHVESGLFTFIGATTENPSFEVNSALLSRAAVYVLKSLSEEDLDALVDRACI
                     QELDGLDFTPEAKAILIASADGDGRKLLNNLEIVARAAAANEAAVVDEALLASALADN
                     LRRFDKGGDTFYDQISALHKSVRGSNPDASLYWFVRMLDGGADPRYLARRIVRMACED
                     IGLADPRALRLALDAAETYERLGSPEGELALAEAVVYLACAAKSNAVYNAYNAARAFV
                     AKDKTRPVPEHLRNAPTKLMKELGYGKLYRYAHDEPHAYAAGENYFPEGMREPGWYQP
                     VPRGLESKIVEKLAWLRELDRNAGK"
     gene            1090..2163
                     /locus_tag="VM406_14230"
     CDS             1090..2163
                     /locus_tag="VM406_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase"
                     /protein_id="HVL77169.1"
                     /translation="MKPAYRLLSLPRFILALVVAFSSQVALAAVGVTALPAPDGNGPV
                     TVFYPTLEPAQNVTRGGAVLQLAPDAAPAPGNGRLVVISHGSGGHPWGHADLAQALVA
                     DGFIVAAPEHHRDNHRDWSDAGPKSWKRRPAEVSMAIDAVARDVRFATILSLDKVGVY
                     GMSAGGHTALSLAGGQWSPARIRRHCEAHINEDFHACVGLTTQLRGNAFDGVRKAFAL
                     WVIGLMANDENHYAHEDGRVAAVVAGVPYAADFDMSTLAAPRVPLALVVARQDRWLVS
                     RFHAERVLAACASCELLADLHNAGHGALLSPFPPRVSGLLADLLRDPPDFDRSVLPEV
                     DRRISAFFRMHLVKPPGALAGTP"
     gene            2229..3524
                     /gene="serS"
                     /locus_tag="VM406_14235"
     CDS             2229..3524
                     /gene="serS"
                     /locus_tag="VM406_14235"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HVL77170.1"
                     /translation="MIDIQLLRKDIDSAAARLASRKFQLDVAGFNALEAERKQIQTRT
                     EELQGKRNALSKQIGMLKGKGEDASAVMAEVAGIGDELKASAERLDVIQARLNEFMLA
                     IPNLPHESVPHGQDESANVEVRRVGTPRTFDFEVRDHVDVGGPLGLDFDVATKLTGSR
                     FAVMKSGIARLHRALAQFMLDTHVNEHGYTECYTPYIVNADSLRGTGQLPKFESDLFA
                     AKKGGQEGEGESLYLIPTSEVPLTNMVRDEIVAADMLPLKMTAHTPCFRSEAGSYGRD
                     TRGMIRQHQFDKVEMVQVVHPEKSYEALEEMVGHAENILNKLGLPYRLITLCTGDMGF
                     GAAKTYDLEVWLPAQKTYREISSVSNCEAFQARRMQARFRNAQGKPELLHTLNGSGLA
                     VGRTLVAILENYQQADGSVEIPPALRPYMGGIERLVRPA"
     gene            complement(3536..>3992)
                     /locus_tag="VM406_14240"
     CDS             complement(3536..>3992)
                     /locus_tag="VM406_14240"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVL77171.1"
                     /translation="FKPFNDVYGYRRGDDIIQLTARVIAMHCDPNRDFVGHIGGDDFI
                     VLFQSGDWETRCRAILDEFHRVSAACFSAADRERGGFVSEDRRGNTVLHPLASLSIGA
                     VRVEPGQYLSHHQIATAAADAKKEAKKISGDSLFIERRHAGLAPLSRNE"
BASE COUNT          679 a         1313 c         1324 g          676 t
ORIGIN      
        1 atcgcgctgt cagccgtcct gtccggcgtc aaggatatcc gcgaggcggt cgaacgggcc
       61 gaactcgtgc gcgcgacctc cgggcgcagg accatcctgt tcgtcgacga agtgcaccgc
      121 ttcaacaaga gccagcagga cgccttcctg ccgcacgtgg aaagcgggct gttcaccttc
      181 atcggtgcga ccaccgagaa cccttcgttc gaggtcaaca gcgccttgct ctcgcgcgcc
      241 gccgtctatg tgctcaagtc gctcagcgag gaggacctgg atgcactggt ggatcgcgcc
      301 tgcatccagg agctcgacgg gctcgatttc acgcccgagg cgaaagcgat cctgatcgcg
      361 agcgccgacg gggacggtcg caagctgctg aacaacctcg agatcgtcgc ccgcgcagca
      421 gcggcgaacg aggcggcggt ggtcgacgag gcgctgctgg ccagcgcgct ggcggataac
      481 ctgcgccgat tcgacaaggg cggtgacacc ttttacgacc agatctcggc gctgcacaag
      541 tcggtgcgcg gctccaatcc ggatgcctcg ttgtactggt tcgtgcgcat gctcgacggc
      601 ggcgccgacc cgcgctatct cgcccggcgc atcgtgcgca tggcgtgcga agacatcggc
      661 ctggccgatc cgcgcgcgct gcgcctggcg ctcgacgcgg ccgaaactta cgagcggctc
      721 ggctcgccgg aaggcgaact cgcgctggcc gaagccgtgg tctacctggc ctgcgccgcg
      781 aagtccaatg ccgtctacaa cgcgtacaac gccgcgcgcg cctttgtcgc gaaggacaag
      841 acgcggccgg ttccggaaca tctgcgcaac gcgccgacca agctgatgaa ggagctcggc
      901 tacggcaagc tgtaccgcta tgcacatgac gagccgcacg cctacgcggc cggggagaac
      961 tactttcccg aggggatgcg cgagccgggc tggtaccagc ccgtgccgcg cgggctggaa
     1021 agcaagatcg tggaaaaact cgcgtggctg cgcgagctgg accgcaatgc gggcaagtag
     1081 ggaggggcga tgaaaccggc ctatcgcctg ctgagcctgc cgcgcttcat tctcgctctc
     1141 gtcgtcgcct tttccagcca ggtcgcgctg gccgccgtcg gcgtgacggc gttgcccgcg
     1201 ccggacggta atggtcctgt caccgtcttc tatccgacgc ttgaaccggc acaaaacgtc
     1261 acgcgcggcg gggcggtgtt gcagctcgcg cccgacgcgg cccccgctcc cggtaacggg
     1321 cggcttgtcg tcatctccca cggctccggc ggccatccct ggggccatgc cgatcttgcg
     1381 caggcgctgg tcgccgacgg cttcatcgtc gccgcgcccg aacaccaccg cgacaaccac
     1441 cgggactgga gcgacgccgg gcccaagagc tggaagcgcc gaccggccga ggtgtcgatg
     1501 gcgatcgatg cagtcgcccg ggatgtccgt tttgcgacca tcctcagcct tgacaaggtc
     1561 ggcgtatacg gcatgtcggc cggcggtcat acggcactct cgctggccgg cggacagtgg
     1621 tcaccggcgc ggatcaggcg tcattgtgaa gcgcacatca acgaagattt tcacgcctgc
     1681 gtcgggctga cgacgcagtt gcgcggcaat gcgttcgacg gcgtgcgcaa ggcattcgct
     1741 ttgtgggtca tcggattgat ggcaaacgac gagaatcatt atgcgcacga ggacggccgt
     1801 gtcgcggcag tcgtcgccgg cgtgccctat gcggcggatt tcgacatgtc gacattggct
     1861 gccccgcgcg tgccgctggc gctcgtcgtt gcgcgtcagg accgctggct ggtgtcgcgc
     1921 ttccacgccg agcgggtgct ggccgcctgc gcgagctgtg aactgctggc cgatctgcat
     1981 aacgccggcc atggcgccct gctatcgcca ttcccgccca gggtctccgg cctgctcgcc
     2041 gacctgctgc gcgatcctcc ggacttcgac cggagcgtac tgccggaggt ggaccgcagg
     2101 atctcggcat ttttccggat gcatctagtc aagccaccag gcgcgctcgc cggcacgccc
     2161 tgacatgcga ggccggcctg gaggcccgcc ttggtaaaat tccgctttcc tccgattccg
     2221 ccatttccat gatcgacatt caacttctcc gcaaggacat cgacagcgcc gccgcccgcc
     2281 tggcttcgcg caagttccag ctcgacgtcg ccggcttcaa cgcgctcgaa gccgaacgca
     2341 agcagatcca gacccgcacg gaagagctgc agggcaagcg caatgcgctg tccaagcaga
     2401 tcggcatgct caagggcaag ggcgaggatg cgagcgcggt catggcggag gtggccggca
     2461 tcggcgacga gttgaaggct tcggccgagc gcctggacgt gatccaggcc cgtctgaacg
     2521 agttcatgct ggccatcccg aacctgccgc acgagtcggt gccgcatggg caggacgaga
     2581 gcgcgaacgt ggaagtgcgc agggtcggga cgccgcgtac tttcgatttc gaggtgcgtg
     2641 accatgtgga tgtcggcggg ccgctgggcc tggatttcga cgttgccacc aagctgaccg
     2701 gctcgcgctt tgcggtaatg aagagcggca tcgcgcgcct gcatcgcgcg cttgcccagt
     2761 tcatgctgga tacgcacgtc aacgagcacg gctacaccga gtgctatacg ccctacatcg
     2821 tcaacgccga ttcgctgcgc ggcaccggcc agctgccgaa gttcgagtcc gacctgttcg
     2881 cggcgaagaa gggcgggcag gaaggcgagg gcgagtccct ctacctgatt cccacttccg
     2941 aggtgccgct gacgaacatg gtgcgcgatg aaatcgtcgc cgccgacatg ctgccgctga
     3001 agatgaccgc gcatacgccg tgcttccgct cggaggccgg cagctacggg cgcgacacgc
     3061 gcggcatgat ccgccagcac cagttcgaca aggtcgagat ggtgcaggtc gtgcatccgg
     3121 agaagtcgta cgaagcgctc gaggaaatgg ttggccatgc cgagaacatc ctcaacaagc
     3181 tcggcctgcc gtaccgcttg atcacgctgt gcaccggcga catgggtttc ggcgcggcca
     3241 agacctatga cctggaggtg tggctgccgg cgcagaaaac ctaccgcgag atttcgtcgg
     3301 tgtcgaactg cgaagccttc caggcgcggc gcatgcaggc gcgctttcgc aatgcgcagg
     3361 gcaagccgga actgctgcat acactgaacg gatcgggact ggcggtcggg cgtacgctgg
     3421 tcgccatcct ggagaactac cagcaggctg acggcagcgt tgagatccct ccggcgctgc
     3481 gtccttacat gggcggcatc gagcggctcg tgcgcccggc ctgagtccgg gtccgctatt
     3541 cgttgcggga gagcggcgcc agtcccgcat ggcgtcgctc gatgaacaag ctgtcccctg
     3601 aaatcttctt cgcttccttc tttgcatccg cggcagccgt tgcgatctgg tggtgcgaca
     3661 gatactgacc cggctccacc cggaccgcgc cgatcgacag gctcgccagc gggtgaagca
     3721 ccgtgttccc gcgcctgtcc tcgctgacga aaccgccgcg ctcgcggtcc gcagcgctga
     3781 agcaggcggc actgacgcga tggaactcgt cgaggatcgc gcggcagcgc gtttcccagt
     3841 cgccgctctg gaacaggacg atgaagtcgt cgccgccgat gtgtccgacg aagtcgcggt
     3901 tcggatcgca gtgcatggca atgacgcggg cggtcaactg gatgatgtcg tccccgcgcc
     3961 ggtagccgta cacatcgttg aacggcttga ag
//
LOCUS       DATLGF010000462        11357 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_122447, whole genome shotgun sequence.
ACCESSION   DATLGF010000462 DATLGF010000000
VERSION     DATLGF010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 11357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11357
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122447"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="VM406_14245"
     CDS             complement(<1..907)
                     /locus_tag="VM406_14245"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HVL77172.1"
                     /translation="MNTSRFRARAGATMRNMMRSLRIRLLLLLGVAIGAAAFLQFTAS
                     FRAAMQEANNLFDYHMQQMAFALQDSGFEEAGWHGMAGANDEDFEFVVQIWTDDGVRV
                     YQSRTYRVLPERAPLGYSNVRLPNGDWRVYAVQASTRVIQVAQRIESRRSRAMALALR
                     ALWPVLAVSVLLFGAAWWVVTSALAPLNRIGHDLANRNADSLAPVSTRGVPHEVSLLV
                     AELNSLLARVAKALQSQQRFVADAAHELRSPLTALRLQVQTLARARDDAARQQAVARL
                     LGGVDRATRLVEQLLVLARQDPAVPE"
     gene            complement(927..1586)
                     /locus_tag="VM406_14250"
     CDS             complement(927..1586)
                     /locus_tag="VM406_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL77173.1"
                     /translation="MKILLVEDDAMIGENIQIALEEEGIAVDWMRDGVAAEAALATQA
                     YDALVLDLGLPGKDGLEVLRTLRGRGKGLPVLIVSARDALADRVLGLKDGADDYLVKP
                     FDLEELIARLHALVRRARGAVEPIYRRGDVAVNTATRQVQRDDQQVILSAREWAILDA
                     LLARPGAILSRSQLEERLYGWSGEVESNAVEVHIHGLRKKLGQQFIVNVRGVGYMVEK
                     T"
     gene            complement(1604..2131)
                     /locus_tag="VM406_14255"
     CDS             complement(1604..2131)
                     /locus_tag="VM406_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77174.1"
                     /translation="MTSWGEALRRGALSGSIASLTSTAALAACGECEDGSPHAPTNAI
                     SHWLWGDRAAREDEPSLRYTGVGYAIHHASATFWAVLYEKWFGRSVERRELRTSVAGA
                     AVVSGLACLVDYTITPPRLRPGYEKRLSAASLCLVYASFGAGLLLRSLATPKPRSGAP
                     ARASPPSARVDTMAI"
     gene            2296..3585
                     /locus_tag="VM406_14260"
     CDS             2296..3585
                     /locus_tag="VM406_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL77175.1"
                     /translation="MKVLLTGATGFIGRHLLRALLDAGHEVRCAVRDPGAINLPGVQA
                     VAADFMRDHDEGAWRGHVAGMDAVINAVGILREEGGQTFDALHVRAPCALFAAAARAG
                     VRLVVQISALGADANAQSRYHLSKKRADDFLATLPVASVILQPSLVYGNDGASARLFA
                     MLAALPLIPLPGKGGQAVQPVHIDDLCAAVLALLRQAPSGSRRLAVIGAQPLALRDYL
                     ALLRKALGMGRGWYVPVPMAAMRLTAGAARFLPGALVDSETLQMLERGNRGSAEDVAR
                     LSRLLGRAPRAPAAFVGDAAPLLAMRAKLDWLLPLLRVSIALVWIVTGIVSLGLYPVA
                     DSYALLARVGATGLLATVLLYGAALLDLAIGIAVLVLRRRPWLWLLQLALIAGYTVII
                     TLRLPEFWLHPYGPILKNLPLMAAIWIMYEFEKSWNT"
     gene            3573..4037
                     /locus_tag="VM406_14265"
     CDS             3573..4037
                     /locus_tag="VM406_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 domain-containing protein"
                     /protein_id="HVL77176.1"
                     /translation="MEYLTVKWLHILSSTILFGTGIGSAFYMLAATMTRDARAVAVTA
                     RHVVLADWLFTTPTIILQPLTGFYLMMLAGMPMSSRWIAWSLILYGIAVLCWLPVVWL
                     QIRMRQSAAAAAEAGRPLDAQYWRLFRLWFALGWPAFLAFLGVFYLMVFKPA"
     gene            complement(4044..5342)
                     /gene="guaD"
                     /locus_tag="VM406_14270"
     CDS             complement(4044..5342)
                     /gene="guaD"
                     /locus_tag="VM406_14270"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HVL77177.1"
                     /translation="MNQDIQAYRASLLHFSADPAFDDNAHAWHEDGLLVVADGRVQAA
                     GDHATLAATLPEGTPVHDLRGKLIVPGFIDTHIHYPQTDMIASPAPGLLPWLETYTFP
                     HERRFQDPEYAGEVARFFLDELLRCGTTTAMVYCTVHKESVDAFFTESEKRNLRMVAG
                     KILMDRNCPDFLCDTAEGGVRDSAELIGRWHNKGRQLYAITPRFAPTSTEAQLRLAGE
                     LARAYPDTFLQTHVAENTDEVAWVKSLFPDSRSYLDVYDSYGMMRPRAMFAHCIWLDD
                     HDRARMRETQSAASICPTSNLFLGSGLFDFKRADEAQVPISLATDVGGGTSFSMLQTM
                     NAVYKVARMGGSYLPALRMFYLATLGGARAMQLEGTIGSFAAGCEADFVVLDPQSTPL
                     LARRTAHADSLEELLFALALLGDDRAVAATYSAGRCVHRR"
     gene            complement(5339..6367)
                     /gene="xdhC"
                     /locus_tag="VM406_14275"
     CDS             complement(5339..6367)
                     /gene="xdhC"
                     /locus_tag="VM406_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HVL77178.1"
                     /translation="MTTWLNAIPTLAAPTVLVTVASAQGSTPREAGAKMLVSADLQFD
                     TIGGGHLELRACEIARAMLQNGQRRHLERFALGPGLGQCCGGAVQLLFERIDEIHDLH
                     RLAERLAARRDVWRAVPLDADDDALLLDREGRMIGAKTDRAPGFDPDLPCRLLKDANG
                     KTWLIDPCRATRNHLVLFGAGHVGAALVRALAELPCHVTWVDEREELFPAEVPANVQI
                     ECTDTPQSVAEQAAPGTSFLVMTHSHALDQALAETILRRREAGWFGLIGSDTKRAQFE
                     RRLREHGIGEERIRTMVCPIGIPGIAGKAPAVVAASVCAQLLQVWEAANSKDADNAAL
                     AKTESESQ"
     gene            complement(6449..8797)
                     /gene="xdhB"
                     /locus_tag="VM406_14280"
     CDS             complement(6449..8797)
                     /gene="xdhB"
                     /locus_tag="VM406_14280"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HVL77179.1"
                     /translation="MNTQTESFLKQLADWAEVGKSRPHESAALHVLGEATYTDDIPEL
                     QGTLHAALGLSQKAHAKIRAIDLEAVKAAPGVVAVYTAADIPGTNDCGPIIHDDPILA
                     DGLVQYVGQPVFLVVADTHDNARRAARLGRIDYEELPAILTPQQAKQAQSFVLPPMRL
                     ARGDFRRAFEAAPHRFSGELYVGGQEQFYLEGQISYAVPKEDRGMHVWCSTQHPSEMQ
                     HVIAHALGLHSHHVVVECRRMGGGFGGKESQSALWAAAASIAAARLNRPVKLRADRDD
                     DMMVTGKRHDFHYEYEIGYDDDGRILAARVEMVSRAGYSADLSGPVATRAVCHFDNAY
                     YLSDVEILAMCGKTHTQSNTAFRGFGGPQGAIAIEYIIDNIARNLGKDPLDVRRLNFY
                     GRNDAEGRNVTQYGQKVVDNVIHELVDQLAAESDYRKRREAVRAFNAASPVLKKGIAI
                     TPVKFGIAFNLTHLNQAGALVHVYVDGSVLVNHGGTEMGQGVNTKVAQVVAHELGLDL
                     GHVRVTATDTSKVANTSATAASTGADLNGMAARNAARQIRDRLTAFCAELFNADPATI
                     RFADNHVWIGDAAEPFPMIVRRAYNARIQLWSDGYYATPGLHWDAATMTGNPFFYFAY
                     GAAVSEVVVDTLTGEWKLLRADALYDAGKSLNPAIDIGQVEGAFIQGMGWLTTEELWW
                     NPAGKLMTHAPSTYKIPAISDCPDQFYVKLFENSNASDSIHRSKAVGEPPLLLPFSVF
                     FAIRDAVASVAHYTVNPPLNAPATSEEILKAVEVVKAVRKAA"
     gene            complement(8808..10286)
                     /gene="xdhA"
                     /locus_tag="VM406_14285"
     CDS             complement(8808..10286)
                     /gene="xdhA"
                     /locus_tag="VM406_14285"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HVL77180.1"
                     /translation="MPEPIRYYYRGQVHTVADAPATRTILQQVREQHHCTGTKEGCAE
                     GDCGACTVVIGELKGDQVELKAVNACIQFLPTLDGKALFTVEDLKQAKDTLHPVQQAM
                     VECHGSQCGFCTPGFVMSLWGLYLKQEGKAAAPTRKEVDDTLSGNLCRCTGYRPIIQA
                     AQRMLELPPVSFDREALAGALRAIQRDHALDYEADGQRFHTPRTLDELCALRAEKPDA
                     LILSGSTDVGLWVTKQMRELGDLIYINQVAELQYVAERGGKLEIGAAVTLNDAYAALA
                     KAYPELSELWQRFASLPIRNAGTLVGNVANGSPIGDSAPWLIAVGSEIVLRSKDGRRV
                     MPLEDFYLDYRKFDLRPGEFVEALRVPLPEPALRFRTYKLAKRFDQDISAVCAAFAIR
                     LEGSKVESARIAFGGMAATSRRARQAEAALAGREWNETTLEAAVEALARDYSPLTDMR
                     ASAEYRMKSAQNLLRRFWLETRPDAPLPAHAVNAFACAFACE"
     gene            10489..10842
                     /gene="uraH"
                     /locus_tag="VM406_14290"
     CDS             10489..10842
                     /gene="uraH"
                     /locus_tag="VM406_14290"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HVL77181.1"
                     /translation="MGKLSTHVLDVTQGRPGAGVALELYRVEGQGRSFLREAVTNSDG
                     RCDAPLLSGEEMQAGQYELVFAVGDYFAAQGLQLPEPRFLDRVIIAFGIADASQNYHV
                     PLVVSPWAYSTYRGS"
     gene            10889..11116
                     /locus_tag="VM406_14295"
     CDS             10889..11116
                     /locus_tag="VM406_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77182.1"
                     /translation="MKRYKNLSRKSGVVAYEVGQRTIVVEFEDGGIYLYNYESAGQDK
                     IERMKQLAEAGRGLSTFIVRHVRDAYAAKLR"
     gene            complement(11119..>11357)
                     /locus_tag="VM406_14300"
     CDS             complement(11119..>11357)
                     /locus_tag="VM406_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HVL77183.1"
                     /translation="LFGKRDKLRRLLRAAGVPRGQVLCIGDEVRDLEAAHQENLDFGA
                     VAWGYTRPDALAAHGPAFLFSQPMEICAALADPK"
BASE COUNT         1728 a         3762 c         3912 g         1955 t
ORIGIN      
        1 cttcggggac ggccgggtcc tggcgcgcca gtaccagcag ctgttcaacc agccgggtgg
       61 cacggtccac cccgcccagc aggcgggcaa ccgcctgctg gcgcgcggcg tcgtcgcgcg
      121 cccgcgccag ggtctggacc tgcaaccgca gcgcggtaag cggggagcgc agttcgtgcg
      181 cggcgtcggc cacgaagcgc tgctgcgact gcagcgcctt ggccacgcgt gccagcagcg
      241 agttgagttc tgccaccagc agcgagactt cgtgcggcac gccgcgtgtg gagaccggcg
      301 caagcgagtc ggcattgcgg ttggccaggt cgtggccgat gcggttgagc ggcgccagcg
      361 cgctggtcac cacccaccag gcggcgccga acaacagcac cgataccgcg agcaccggcc
      421 acagtgcgcg cagcgccaac gccattgcgc gcgagcggcg cgattcgatg cgctgggcga
      481 cctggatcac gcgcgtgctg gcctggaccg catagacgcg ccagtcgccg ttgggcagcc
      541 gcacgttcga atagccgagc ggagcgcgtt ccggcagcac ccggtaggtg cgcgactggt
      601 agacgcgcac gccgtcgtcg gtccagatct gcacgacgaa ttcgaaatcc tcgtcgttgg
      661 cgccggccat gccgtgccag cccgcttcct cgaagccgct gtcctgcagc gcgaacgcca
      721 tctgctgcat gtgatagtcg aacaggttgt tcgcctcctg catcgccgcg cggaacgagg
      781 cggtgaactg caggaatgcg gccgcgccga tggccacgcc gagcagcagc agcaggcgga
      841 tgcgcagcga gcgcatcatg ttgcgcatgg tggcgccggc acgggcgcga aagcgtgagg
      901 tgttcatggt tgccgccttg ccggcctcag gtcttctcga ccatgtagcc gaccccgcgc
      961 acattgacga tgaactgctg gccaagcttc ttgcgcagcc cgtggatgtg cacttccacc
     1021 gcattgcttt ccacttcgcc ggaccagccg tacaggcgct cttccagctg gctgcgcgag
     1081 agaatcgcgc ccgggcgcgc cagcagcgcg tcgaggatcg cccattcgcg tgcagacagg
     1141 atcacctgct ggtcgtcgcg ctgcacctgg cgcgtggcgg tgttgacggc gacatcgccg
     1201 cgccggtaga tcggctcgac cgcgccgcgt gcgcgccgta ccagcgcatg caggcgggcg
     1261 atcagttcct ccagatcaaa aggtttgacc aggtagtcgt cggcgccgtc cttcagtccg
     1321 agcacgcggt ccgcgagtgc gtcgcgcgcg ctgacgatca gcaccggcag gcccttgccg
     1381 cgtccgcgca gcgtgcgcag cacttccaga ccgtccttgc cgggcagacc gaggtccagc
     1441 accagcgcgt cgtaggcctg ggtggccagc gccgcctccg cggccacgcc gtcgcgcatc
     1501 cagtcgaccg cgatgccttc ttcctccagc gcgatctgga tgttctcgcc gatcatggcg
     1561 tcgtcttcga cgagcaggat tttcatggaa tggtgcgctc agttcagatc gccatggtat
     1621 caactctggc cgaaggaggc gatgcgcgcg ccggtgcgcc ggagcgcggt ttcggggtcg
     1681 cgaggctgcg cagcagcagg ccggcgccga aggatgcgta gaccaggcac agcgaggccg
     1741 cggacagccg tttctcgtag ccggggcgca ggcgcggcgg cgtgatcgtg taatcgacca
     1801 ggcaggccag gccggacacc acagccgccc cggcaaccga ggtgcgcagc tcgcggcgct
     1861 cgaccgagcg gccgaaccat ttctcgtaga gcacggccca aaacgtggcc gaggcatggt
     1921 gtatcgcata gccgaccccg gtgtagcgca ggctcggctc atcctcgcgc gcggcacgat
     1981 cgccccacag ccagtggctg attgcgttcg tgggggcatg cggcgatcca tcctcgcatt
     2041 cgccgcaagc cgccagcgcg gcggtcgaag tcaggctcgc gatgcttccg gacagcgcgc
     2101 cgcgccgcaa cgcttctccc cagctggtca tgtttcctcc tcgaaaattc ccggagcacg
     2161 ggtccggcgg gaccggcgct tgaactcctc cccccgtgca acagtcacaa agcagggaga
     2221 ccgaccgcgt gtcgcagcac gggttcgaaa gcctcgtccc ggcggcagtt cccgctttca
     2281 acaacgcgaa gatcaatgaa agtcctgctc accggcgcga ccggcttcat cggccgccac
     2341 ttgctgcgcg cgctgctcga tgccggtcac gaggtgcgct gcgcggtgcg cgacccgggc
     2401 gcgataaacc ttcccggcgt gcaggccgtt gccgcggact tcatgcgcga ccacgacgag
     2461 ggcgcctggc gcggccatgt cgccggcatg gatgcggtga tcaacgcggt gggcatcctg
     2521 cgcgaggagg gcggccagac cttcgacgcg ctgcatgtgc gtgcgccctg tgcgctgttt
     2581 gcggccgccg cgcgagcggg agtgcggctg gtcgtgcaga tctcggcact cggcgcggac
     2641 gccaacgcgc agagccgcta ccacctgagc aagaagcgcg cggatgattt tctcgcaacc
     2701 cttccggtgg cctccgtgat cctgcagcct tcgttggtgt acggcaacga cggggcgagc
     2761 gcgcgcctgt tcgcgatgct cgccgcgctg ccgctgatcc cgttgccggg caagggcggg
     2821 caagcggtgc agcctgtcca catcgacgac ctgtgcgccg cggtgctggc cttgctgcgg
     2881 caggcgccgt ccggaagccg gcgcctcgcc gtgatcggag cgcagccgct ggcgctgcgc
     2941 gactatctgg cgctgctgcg caaggcgctg ggcatgggcc ggggatggta cgtaccggtg
     3001 ccgatggcgg cgatgcgcct gacagcgggc gccgcacgct tcctgccggg cgcgctggtg
     3061 gacagcgaga cgctgcaaat gctcgagcgc ggcaaccgcg gcagcgccga ggacgtggcg
     3121 cggctgtccc gcctgctcgg ccgtgcgccg cgtgccccgg cggcattcgt cggcgatgcc
     3181 gcgccgctgc tggcgatgcg ggcgaagctg gactggctgc tgccgctgct gcgcgtatcg
     3241 atcgcgctgg tctggatcgt gaccggcatc gtttcgctcg gactgtatcc ggttgccgac
     3301 agttacgcgc tgctggcgcg ggtgggcgcg accggcctgc tcgccaccgt gctgctgtac
     3361 ggcgcggcat tgctggatct ggcgatcggc atcgcggtcc tcgtgctgcg ccggcgcccg
     3421 tggctgtggc tgctgcagct ggcgctgatt gcggggtaca cggtgatcat cacgctgcgg
     3481 ctgcctgaat tctggctgca cccgtacgga ccgatcctga agaacctgcc gctgatggcg
     3541 gcgatctgga tcatgtatga gttcgaaaag tcatggaata cctgaccgtc aagtggctgc
     3601 acatcctgtc gtcgacgatc ctgttcggca ccggcatcgg ttcggccttc tacatgctgg
     3661 cggcgacgat gacgcgcgac gcgcgcgcgg tggccgtgac ggcacgccat gtggtgcttg
     3721 ccgactggct gttcaccacg cccaccatca tcctgcagcc gctcaccggc ttctacctga
     3781 tgatgcttgc cggcatgccg atgagcagcc gatggatcgc gtggtcgctg atcctgtacg
     3841 ggatcgccgt gctgtgctgg ctgccggtgg tgtggctgca gatccgcatg aggcagagcg
     3901 ccgccgccgc ggccgaggcc gggcgcccgc tcgatgcgca gtactggcgg ctgttcaggt
     3961 tatggttcgc gctcggctgg ccggccttcc tggcgtttct gggcgtgttc tacctgatgg
     4021 tgttcaagcc cgcatagccg gcgtcagcgc cgatgcacgc accggcccgc gctgtaggtc
     4081 gcggccacgg cgcggtcgtc gccgagcagg gccagcgcaa acagcagctc ctccaggctg
     4141 tcggcatggg cggtacggcg cgccaggagc ggcgtggact gcgggtcgag cacgacgaaa
     4201 tcggcttcgc agccggcggc gaagctgccg atcgtgcctt ccaattgcat cgcgcgcgcg
     4261 ccgcccagcg tggcgagata gaacatgcgc agcgccggca gatagctgcc gcccatgcgc
     4321 gcgaccttgt agaccgcgtt catcgtctgc agcatcgaaa atgatgtgcc gccgccgacg
     4381 tcggtggcga gcgagatcgg cacctgtgcc tcgtcggcgc gcttgaaatc gaacaggccg
     4441 ctgccgagga agaggttgga ggtcgggcag atcgaggcgg ccgactgcgt ctcgcgcatg
     4501 cgcgcgcggt cgtggtcgtc cagccagatg cagtgcgcga acatcgcgcg cggacgcatc
     4561 atgccgtagc tgtcgtacac gtcaagatag ctgcgcgaat ccgggaacag cgatttcacc
     4621 catgccacct cgtcggtgtt ttccgccacg tgcgtctgca ggaaggtatc gggatacgcg
     4681 cgcgcgagtt cgccggccag ccgcagctgc gcttcggtgg aggtcggcgc gaagcgcggc
     4741 gtgatcgcat acagctggcg tcccttgttg tgccagcggc cgatcagttc cgcgctgtcg
     4801 cgcacgccac cctcggcggt gtcgcacagg aagtccgggc agttgcggtc catcaggatt
     4861 ttcccggcca ccatgcgcag gttgcgcttc tcgctttcgg tgaagaaggc atcgaccgac
     4921 tccttgtgca ccgtgcagta gaccatcgcg gtggtggtgc cgcagcgcag gagttcgtcg
     4981 aggaagaagc gcgcgacctc gcccgcgtat tccgggtcct ggaagcggcg ctcatgcggg
     5041 aaggtatagg tctccagcca tggcagcagt ccgggcgccg gcgaggcgat catgtcggtc
     5101 tgcggatagt ggatgtgggt gtcgatgaag ccgggcacga tcagcttgcc gcgcaagtcg
     5161 tgcaccggcg tgccttcggg cagcgtcgcg gcgagtgtcg catggtcgcc tgcggcctgc
     5221 acgcggccgt cggcgacgac gagcaggcca tcctcatgcc aggcatgggc gttgtcgtcg
     5281 aaggccggat cggcggaaaa atgcagcagg ctggcccggt aggcctggat gtcttggttc
     5341 attgagattc gctttccgtt tttgcgagcg ccgcattatc ggcgtctttg ctgttcgccg
     5401 cttcccagac ctggagcagt tgcgcgcaga cggatgccgc aacgaccgcc ggcgccttgc
     5461 cggcaatgcc cggaatgccg atcgggcaca ccatcgtccg gatccgttcc tcgccgatgc
     5521 cgtgctcgcg cagccggcgc tcgaactgcg cgcgcttggt gtcggagccg atcaggccga
     5581 accagccggc ttcacggcgg cgcaggatgg tctcggcaag cgcctggtcc agagcatgac
     5641 tgtgggtcat caccaggaag ctggtgcccg gcgcggcctg ctcggccacg gactgcggcg
     5701 tatcggtgca ttcgatttgc acgttcgccg gcacctcagc gggaaacagt tcctcgcgct
     5761 cgtccaccca ggtgacatgg cagggcagct ccgcgagcgc gcgcaccagt gcggcgccga
     5821 catgcccggc gccgaacagc acgaggtggt tgcgggtggc gcggcaagga tcgatcagcc
     5881 aggtcttgcc attcgcatcc ttcagcaggc ggcagggcaa gtcggggtcg aagccgggag
     5941 cccggtccgt ttttgccccg atcatgcggc cctcacggtc gagcagcagc gcatcgtcat
     6001 cagcgtccag cgggaccgcg cgccagacgt cgcgccgcgc cgcgagccgt tcggccagcc
     6061 ggtgcaggtc gtgtatttca tcgatgcgct cgaacagcag ctggaccgcg ccgccgcagc
     6121 actggccgag gccgggtccg agcgcgaagc gctccaggtg ccggcgctgc ccattctgca
     6181 acatcgcgcg cgcgatctcg cacgcgcgca gctccaggtg gccgccgcct atcgtgtcga
     6241 actgcaggtc cgcgctgacc agcatcttcg cgccggcttc gcgcggcgtc gatccctgtg
     6301 ccgaggcgac cgtgaccagc acggttggcg cggcgagcgt ggggattgcg ttgagccagg
     6361 tggtcatcgt ggtgtttcgt ctcttggcgt gcggatgagg tccctcgctg tgctcgggac
     6421 gacatgtctt tctccggcag cgtgggcctc aggccgcctt gcgtaccgcc ttcactacct
     6481 ccaccgcctt caggatttcc tcgctggtcg ccggcgcgtt cagcggcgga tttacggtgt
     6541 agtgcgccac gctcgcgacc gcgtcgcgga tcgcgaagaa taccgagaac ggcagcagca
     6601 gcggcggttc gccgacggcc ttggagcggt gaatgctgtc ggacgcattg ctgttctcga
     6661 acagcttcac gtagaactgg tccgggcagt ccgagatcgc cggaatcttg taggtggacg
     6721 gcgcatgcgt catcagcttg ccggccgggt tccaccacaa ttcctcggtg gtcagccagc
     6781 ccatgccctg gatgaacgcg ccctcgacct ggccgatgtc gatggccggg ttcagcgact
     6841 tgcccgcgtc gtacagcgcg tcggcgcgca gcagcttcca ttcgccggtc agtgtgtcga
     6901 ccaccacctc cgagaccgcc gcgccatagg cgaaatagaa gaacggattg ccggtcatcg
     6961 tcgccgcgtc ccagtgcaag ccgggcgtcg catagtagcc gtcggaccag agctggatgc
     7021 gcgcgttgta cgcacggcgc acgatcatcg ggaagggctc ggccgcatcg ccgatccaga
     7081 catggttgtc ggcgaagcgg atggtcgccg gatcggcgtt gaacagttcc gcgcagaatg
     7141 cggtgaggcg gtcgcggatc tggcgtgcgg cgttgcgcgc cgccatcccg ttcaggtcgg
     7201 cgccggtgga agcggcggtg gccgaggtgt tggcgacctt gctggtgtcg gtggccgtca
     7261 cccgaacatg gccgaggtca agcccgagtt catgcgcgac gacctgcgcg accttggtgt
     7321 tgacgccctg gcccatctcg gtgccgccgt ggttcaccag caccgaaccg tcgacataca
     7381 catggaccag cgcgccggcc tggttcaggt gggtcaggtt gaacgcgatg ccgaacttca
     7441 ccggcgtgat cgcgatgccc ttcttcagca ccgggctggc ggcgttgaac gcgcgcaccg
     7501 cctcgcgccg cttgcgatag tcgctttccg cggcgagctg gtccaccagc tcatggatca
     7561 cgttgtcgac gaccttctgg ccgtactggg tgacattgcg cccttcggcg tcgtttcggc
     7621 cgtagaagtt gagccggcgc acatccagcg gatccttgcc caggttgcgc gcgatgttgt
     7681 cgatgatgta ttcgatcgca attgcgccct gcggaccgcc gaagccgcgg aacgcggtgt
     7741 tggactgcgt gtgcgtcttg ccgcacatcg cgaggatctc gacgtccgac aggtagtagg
     7801 cgttgtcgaa atggcagacc gcgcgggtcg caaccggacc ggacaggtcg gcagagtagc
     7861 cggcgcgcga gaccatctcc acgcgtgcgg ccaggatgcg gccgtcgtcg tcgtagccga
     7921 tttcgtattc gtagtggaag tcgtggcgct tgccggtgac catcatgtcg tcgtcgcggt
     7981 cggcgcgcag cttcaccggg cgattcagcc tggccgccgc gatcgatgcg gccgccgccc
     8041 acagcgcgga ctgcgattcc ttgccgccga agccgccgcc catgcgccgg cactcgacca
     8101 ccacatggtg cgaatgcagg ccgagcgcgt gtgcgatcac atgctgcatc tcggacgggt
     8161 gctgcgtcga gcaccagaca tgcatgccgc ggtcctcctt cggcaccgca taggaaatct
     8221 ggccttccag atagaactgt tcctgcccgc cgacgtacag ctcgccgctg aagcggtgcg
     8281 gcgccgcttc gaacgcacgg cggaagtcgc cgcgcgccag ccgcatcggc ggcagcacga
     8341 aggattgcgc ctgcttcgcc tgctgcggcg tcaggatggc cggcagctcc tcgtagtcga
     8401 tgcgacccag ccgcgcagcg cggcgcgcat tgtcatgcgt gtcggccacg accaggaaca
     8461 ccggctggcc cacgtactgc acgaggccgt cggccaggat gggatcgtca tggatgatcg
     8521 gtccgcagtc gttggtaccg ggaatgtcgg ccgcggtgta gaccgcgacc acgcccggcg
     8581 cggccttgac cgcctccaga tcgatggccc ggatcttcgc gtgcgccttt tgcgacaagc
     8641 cgagcgccgc atgcagcgtg ccttgcagct ccgggatgtc gtcggtatag gtggcttcac
     8701 cgagcacatg cagggcagcg gattcatgcg ggcgcgactt gccgacctcg gcccagtcgg
     8761 cgagctgctt caggaaggat tcggtctgcg tgttcatcgt cgtgtcctca ttcgcaggca
     8821 aaggcgcagg caaatgcatt gaccgcgtgc gccggcagcg gggcgtcggg gcgcgtctcc
     8881 agccagaagc ggcgcaacag gttctgcgcg ctcttcatcc ggtattcggc cgacgcgcgc
     8941 atgtcggtca gcgggctgta gtcgcgggcg agggcttcga ccgcggcttc cagcgtcgtc
     9001 tcgttccatt cgcgcccggc cagagcggcc tcggcctggc gtgcgcggcg cgaggtggcc
     9061 gccatgccgc cgaaggcgat gcgcgcgctc tccaccttgc tgccttcgag gcggatcgcg
     9121 aacgcggcgc acaccgccga gatgtcctgg tcgaagcgct tggccagctt gtaggtgcga
     9181 aaacgcagcg cgggttcggg caggggcacg cgcagcgcct cgacgaattc acccggccgc
     9241 aggtcgaact tgcggtaatc gagatagaag tcttccagcg gcatcacgcg ccggccatcc
     9301 ttgctgcgca gcacgatctc gctgccgacc gcgatcagcc acggcgccga gtcgccgatc
     9361 ggggaaccgt tggcgacgtt gccgaccagg gtgccggcat tgcggatcgg cagcgatgca
     9421 aagcgctgcc agagttcgct cagttcagga taggctttcg ccagcgcggc gtacgcgtcg
     9481 ttgagggtga ctgccgcgcc gatctccagc ttgccgccgc gctcggcgac gtactgcagc
     9541 tcggcgacct ggttgatgta gatcaggtct cccagttcac gcatctgctt cgtcacccac
     9601 agtccgacgt cggtggagcc ggacaggatc agcgcatcgg gtttctcggc gcgcagcgcg
     9661 cacagttcgt ccagcgtgcg cggcgtgtgg aagcgctggc catcggcctc atagtcgagc
     9721 gcgtggtcgc gctggatcgc ccgcagcgcg ccggccagcg cctcgcggtc gaacgagacc
     9781 ggcggaagct cgagcatgcg ttgcgcggcc tggatgatag ggcggtaccc ggtgcagcgg
     9841 cacaggttgc cggagagcgt gtcgtcgacc tccttgcgtg tcggcgcggc agccttgcct
     9901 tcctgcttca ggtacagccc ccacagcgac atcacgaagc cgggcgtgca gaagccgcac
     9961 tgcgaaccgt gacattcgac catcgcctgc tgcaccgggt gcagggtgtc cttggcctgc
    10021 ttcaggtcct cgacggtgaa cagcgccttg ccgtccagcg tgggcaggaa ctggatgcag
    10081 gcgttgacgg ccttgagctc gacctggtcg cccttgagct cgccgatgac caccgtgcag
    10141 gccccgcagt cgccttccgc acagccttcc ttggtgccgg tgcaatgatg ctgctcgcgt
    10201 acctgctgca ggatcgtgcg ggtcgcaggg gcatcggcca cggtatggac ttgtccgcgg
    10261 tagtagtagc gaatcggttc tggcatgctg tctccatgat gggcaagggc gcgcgcggcc
    10321 ctgcggccgc cgtccagcgt ggtcactcta gcaccggtgc gcgccctcgc catatcggaa
    10381 acgtgatgag aggtattcac accgcgcaat gccggctata tgcgacctcg catacctgcc
    10441 acctttctcc ccgatatgat gagaggaatt tttgacaagg agaacccgat gggaaaactg
    10501 agcacccatg tgcttgatgt cacgcaaggc aggccgggcg ccggcgtcgc gctcgagctc
    10561 taccgcgtcg aagggcaggg ccgcagcttc ctgcgcgagg cagttaccaa cagcgacgga
    10621 cgctgcgacg cgcctttgct gtccggcgag gagatgcagg ctggccagta cgaactcgtg
    10681 ttcgccgtcg gcgactattt cgctgcgcaa gggctgcagc tgcccgagcc gcgctttctc
    10741 gaccgcgtga tcattgcctt cggcattgcc gatgcgtccc agaactacca tgtgccgctg
    10801 gtggtgtcgc cgtgggcgta ctccacctat cggggcagct gaggcggcgt ctgccggatt
    10861 gcccgcgaat ttccgcgcga ggtgcgggat gaagcgctac aagaatctga gtcgcaagtc
    10921 cggcgtcgtt gcctacgagg tcgggcagcg cacgatcgtc gtcgagttcg aggacggcgg
    10981 gatctatctg tacaactacg agagcgccgg tcaggacaag atcgagcgga tgaagcagct
    11041 ggccgaggca ggacggggcc tgagcacctt catcgtgcgc cacgtgcgcg acgcttatgc
    11101 cgccaaactg cgctgatgct atttcggatc ggcaagcgca gcgcagattt ccatcggctg
    11161 gctgaacaga aaggccggcc catgcgcggc cagcgcatcg ggccgtgtat aaccccacgc
    11221 cactgcaccg aaatccaggt tctcctggtg cgctgcttcc agatcgcgca cctcgtcccc
    11281 gatgcacagg acctgcccgc gcggcacgcc ggccgcgcgc aataggcggc gcagcttgtc
    11341 gcgcttgccg aacagcg
//
LOCUS       DATLGF010000463        20965 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_216572, whole genome shotgun sequence.
ACCESSION   DATLGF010000463 DATLGF010000000
VERSION     DATLGF010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 20965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20965
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216572"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /gene="ftsB"
                     /locus_tag="VM406_14305"
     CDS             complement(<1..293)
                     /gene="ftsB"
                     /locus_tag="VM406_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HVL77184.1"
                     /translation="MRLITLSLAALLILIQYPLWLGKGGWLRVWDLDGQVTAAQRKND
                     ELRARNAMLESEVRDLREGTAAVEERARYELGMIKQGEIFIQILEPNSRVPDVA"
     gene            complement(362..1645)
                     /gene="eno"
                     /locus_tag="VM406_14310"
     CDS             complement(362..1645)
                     /gene="eno"
                     /locus_tag="VM406_14310"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HVL77185.1"
                     /translation="MSAIVDIIGREVIDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     SREAIELRDGDKSRYMGKGVLKACENINTEISEAIMGLDANEQAFLDRTLLDLDGTDN
                     KSRLGANAMLAVSMAVAKAAAEESGLPLYRYFGGSAAMQMPVPMMNVINGGAHADNNL
                     DLQEFMIIPVGAPSFREAIRYGAEVFHTLKKILHDKGLTTAVGDEGGFAPSVANHEEA
                     IKLILQAIEQAGYEPGTQIALGLDCAASEFYKDGKYNLEGEGLQLSSADFTNLLATWC
                     DKYPIISIEDGMAENDWDGWATLTNALGKKVQLVGDDLFVTNTKILREGIQKNIANSI
                     LIKINQIGTLTETFAAIEMAKRAGYTAVISHRSGETEDSTIADIAVGTNALQIKTGSM
                     SRSDRMAKYNQLLRIEEDLGDIASYPGRNAFYNLR"
     gene            complement(1718..2572)
                     /gene="kdsA"
                     /locus_tag="VM406_14315"
     CDS             complement(1718..2572)
                     /gene="kdsA"
                     /locus_tag="VM406_14315"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HVL77186.1"
                     /translation="MKLCGFEAGLDQPFFLIAGPCVIESQQMALDTAGTLKELTAGLG
                     IPFIYKSSFDKANRSSGSSFRGLGMEKGLGILAEVKRQIGVPVLTDVHEIDEVEPVSE
                     VVDVLQTPAFLCRQTDFIRACAQSGKPVNIKKGQFLAPGDMKNVIDKAREAAREAGLN
                     ADNFMACERGVSFGYNNLVSDMRSLAIMRETGCPVVFDATHSVQLPGGQGTSSGGQRE
                     FVPVLARAAVAVGIAGLFMETHPNPAEAKSDGPNAVPLKRMKALLATLVELDRVVKKN
                     GFAENDFA"
     gene            complement(2569..4230)
                     /locus_tag="VM406_14320"
     CDS             complement(2569..4230)
                     /locus_tag="VM406_14320"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HVL77187.1"
                     /translation="MTKFVFVTGGVVSSLGKGIAAASLAAILESRGLKVTLIKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFITAKMKKLNNFTTGQIYESVIRKE
                     RRGEYLGKTVQVIPHITNEIQDFIHRGAEGFDVALVEIGGTVGDIESLPFLEAARQLS
                     LRAGRQETAFVHLTLVPYIASAGELKTKPTQHSVQKLREIGISPDALLCRADRRIPDD
                     ERAKISLFSNVREDAVISVWDADTIYKIPQMLHDQGLDRIVCEKLGIDAPPADLSVWN
                     KLVYALEHPKQDVTIGMVGKYVDLTESYKSLTEALRHAGIHTESRVNIEYIDSEEIEV
                     RGTSMLEKYDAILVPGGFGKRGVEGKIAAAKYAREQKVPYLGICLGMQVALIEYARNK
                     AGLKNANSTEFEPDTEHPVVALITEWQNHDGKVERRNASSDLGGTMRLGAQTCAVKPG
                     TIAAEIYGDKVTERHRHRYEANNHYLPRVEEAGLIVSARTPTEGLCEIMELARTGDDA
                     HPWYVGVQYHPEFKSTPRDGHPLFISFIKAALVHKQAASPRRIAA"
     gene            complement(4370..5299)
                     /locus_tag="VM406_14325"
     CDS             complement(4370..5299)
                     /locus_tag="VM406_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HVL77188.1"
                     /translation="MNNILRSSAAGLLAAITLPVAAGTLVDDYRQVYAHGTASHQVDI
                     DNDSLLLTQRDGFYTSGVRYAYRRSTASASEVRVAGWRLGQELYTPSNINLPPALVGP
                     PDRPYAGWLYGGVFHEVYRADGTHVRYGLDLGCLGPCAGGEWTQTRLHRLLDQPEPRG
                     WARQVRNEAGVQLYAETAPVRWQLGRQLDATPVLRGRLGNIFTDAGAGITVRAGRLDA
                     LPGQRTLHAFLRADANLVVYNATMQGGLFSRNDPHTVDPKRVYGEVELGAAWRDGPFG
                     LRVGVVHRTNEVHGLPNSVGAQNYLRLQLLYAP"
     gene            complement(5296..6096)
                     /locus_tag="VM406_14330"
     CDS             complement(5296..6096)
                     /locus_tag="VM406_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL77189.1"
                     /translation="MKRITIHFSHANSYPAGTYTVLFEQMKKHYDVHALDMHAHNPAY
                     PVTNGWSWLERELADELHKRYREPVVLVGHSLGGMLSLMAAKARPELVRAVVLLDSPV
                     VAGWRAAIWGAIKPLKLADRFSPARLSERRRNVWPDAEAAWRHFASKDVFARWDRRVL
                     RDYLKHGLVPHPEGVTLRFSRETETAIYRSLPHHLGKLASRSYPVPVGFIGGHDSVEC
                     RQAGLAATRRLVGRHFMQVPGGHLFPMEAPAETAKAVHKMIHSLLNKP"
     gene            complement(6188..8602)
                     /locus_tag="VM406_14335"
     CDS             complement(6188..8602)
                     /locus_tag="VM406_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HVL77190.1"
                     /translation="MRAALVGFAAGAAVLQRQAELLPVFILIGTLLLALAGAWLARRA
                     PVQVRVVSFAACGIIAGFAWAGLFAHAHLARALAPQWEGTDVVLVGTVDSLPYRFDRG
                     IRFNLAVEQVDGQPLPAGFPARVALSWYAGFRPAERQAVPEVQPGERWQLVTRLRPPH
                     GNANPHGFDYEVWLLEQKLRATGYVRPDQRLEQKNLRLDAFVPGFNAVVQRARAWMRE
                     RIYRALPEHEYAGVIVALVIGDQRAIAQSDWTVFNRTGISHLVSISGLHITMISALFA
                     SLVLALWRRSFFTGAQLPLLLPARKAAALAGAGAALLYVLLAGFGIPAQRTLTMLSVV
                     AAALWFGRITAISHVLCIALGVVVLLDPWAVLQPGFWLSFGAVAAILYSTAGRATTGE
                     AGRGWWQRTLHNLRGAAQVQYAVTLALVPLTMLLFAQVSLVSPLANAVAIPLVSFLVT
                     PAALTGSVLPMPAAEWVLGAAHWLVLGLARVLHWMSALPQAVWTAPEPSWWVFAIAAL
                     GAVWLLAPRGWPVRMLGVACMVPLMLPGAAAPRAGEMWVTAFDVGQGMALLVETPTRR
                     LLYDTGPAYSPESDGGNRVILPYLKGRGIHALDAIVISHSDVDHSGGALSILGEIDVG
                     WVSSSLDPGHRIVQAAREHSRCEAGQAWEWDGVQFEMLYPVAAVYESAKWKPNARSCT
                     LKVTLGRHAILLPGDIEAVQEGHLLSAGPERLRAHVLLAPHHGSGTSSTLPFLQAVQP
                     EIALFQVGRANRYQHPKDEIYRRYGELGAERLRTDETGAVTLRFGEEVAVSAYRQEHA
                     RYWRQR"
     gene            complement(8604..9398)
                     /locus_tag="VM406_14340"
     CDS             complement(8604..9398)
                     /locus_tag="VM406_14340"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HVL77191.1"
                     /translation="MWIDTHCHLDASEFEGEFAEVAARAAIQGVRQIVIPAVGVVNFD
                     AVRRLAAENPNCSYALGIHPIFVPQANDDDLDRLRAAVQAAMDDPRFVAVGEIGLDFF
                     IPGLAQGPMREKQERFYSEQLKIARDFGLPVLLHVRRSQDIILKYLRRTEVRKGIAHA
                     FNGSFQQAEEFIRLGFKLGFGGAMTFPRALQIRRLAGQLPLDALVLETDAPDIPPAWI
                     HPGRNSPEQLPRIGAVLAELRGSDAGRIAEATTANALAALPRIVGL"
     gene            complement(9399..10085)
                     /gene="lolD"
                     /locus_tag="VM406_14345"
     CDS             complement(9399..10085)
                     /gene="lolD"
                     /locus_tag="VM406_14345"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HVL77192.1"
                     /translation="MDQPVLSCQALGKTFTQGKYSVEVLRGIDFSVYRGERVAIVGAS
                     GSGKSTLLHLLGGLDLPTAGTVSLLGQELARLSEKQRGEVRNRSLGFVYQFHHLLPEF
                     SALDNVAMPLMIRRMSRSAAHDEARKILARVGLAARVTHVPGELSGGERQRVALARAL
                     VTQPACVLADEPTGNLDRVTAQKTFDLMLELSRTLGTAFVIVTHDIELARHCDRVLRL
                     TEAGLHPYSE"
     gene            complement(10091..11356)
                     /locus_tag="VM406_14350"
     CDS             complement(10091..11356)
                     /locus_tag="VM406_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HVL77193.1"
                     /translation="MRFTRNLPFEWLVGLRYTRAGRRSGRNSFISFISLISMFGIALG
                     VAALIIVISVMNGFQTEVRDRMLSVLSHIEVFDAYGSLPDWQATAEETLRHREVIGAA
                     PYIAAQAMLTRGDSVRGTVIRGILPEQEPKVSDVADQVVEGSVAALQPGEFNVVLGSE
                     LARGLGVHVGDRVTMIAPQGQVTPAGVLPRLRQFTVAGIFRAGHNEYDAGLAFIHIDD
                     AARMFQQTGPTGLRLRIADMHRAPQVALELSRTLTGNLLILDWSRQNRTWFAAVQVEK
                     RMMFIILTLIIAVAAFNLVSSLVMTVTDKQADIAILRTLGAAPGSIMKIFIIQGALVG
                     LLGTALGASAGVLVAQNIDVIVPFIERVLGVQFLDKDIYLITELPSDLRWSDVGTISG
                     IAVLLAFAATLYPSWRAANVRPAEALRYE"
     gene            complement(11543..11896)
                     /locus_tag="VM406_14355"
     CDS             complement(11543..11896)
                     /locus_tag="VM406_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77194.1"
                     /translation="MPAGASPKREREYKKLEKRFKQEHRYPGREEEVASRIVNKQRSQ
                     YGETRTEKQKDKQGRSPDRGLPIEGYQQMTIAQISKEMEKLSAADIRKLRSYEVKHKN
                     RKGMLERMDRMLQRH"
     gene            12018..12386
                     /locus_tag="VM406_14360"
     CDS             12018..12386
                     /locus_tag="VM406_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77195.1"
                     /translation="MRLFKLDHLVSMIAAPIIWAAHFLICYTMVSLVCMVGWTHARLF
                     GMNPGQIAVALATVVGVVLLGYTGVVNLEKYRQSRKDETGTIEVSGFVALNALLLSIL
                     STVAVVWVAFPTLMLPTCAI"
     gene            12374..13279
                     /locus_tag="VM406_14365"
     CDS             12374..13279
                     /locus_tag="VM406_14365"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HVL77196.1"
                     /translation="MRDLNMKLALLAAALFPASAHAHGEPGAHHWWTLDPWITIPLAG
                     WLLLYARGVQLLRDHNRRTGRSLRVVQAPALAAAALGFASLFLALIWPLDALSADSFA
                     AHMAQHMVLIALAAPLLVYAEPSVPVTRALPALRVVGRSRVLNRLLRIGLKPRVAFAV
                     HGTVIWFWHAPTVFELAVRNDFVHALGHIAFFGSALLFWASLRQVGRAGGDGYGVAAL
                     WTLGTMMHMGFLGGIITFAPRVLYDVYADGGIGGLSPMEDQQLAGLIMWVPAALPYLV
                     AGMGFILAWLRDADRHAPRVQWRRD"
     gene            complement(13321..15633)
                     /locus_tag="VM406_14370"
     CDS             complement(13321..15633)
                     /locus_tag="VM406_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HVL77197.1"
                     /translation="MTTESQLGKDNQLREAALEYHRSPTRGKIEVNPTKALSNQRDLS
                     LAYSPGVAYACEEIAKDPAMAAEYTSRGNLVAVITNGTAVLGLGDIGPLAGKPVMEGK
                     GCLFKKFAGIDVFDIELAEHDPDKLVEAIAMLEPTVGGINLEDIKAPECFYIEKQLRE
                     RMNIPVFHDDQHGTAIISAAALLNGLKVVDKKIEEIKLVASGAGAAAIACLDLMVSLG
                     VRRENIFVADSRGVIWIGRDAKMEENKARYAQDTNARTLADIVAGADVFLGCSTAGVL
                     TGEMVKTMADRPIILALANPEPEIRPEVAKAARPDCIIATGRSDYPNQVNNVLCFPYI
                     FRGALDCGATKITDEMKLACVKAIAELAEAETNDAVAQAYAGQDLTFGPEYIIPKPFD
                     QRLIEKIAPAVAKAAAESGVATRPIQNLDAYRQQLSQLVYHTGMIMKPVFSAAKSAPK
                     RVAYAEGEEERVLRAAQTVVDEGLAKPVLIGRPHVIEMRIQKAGLRLKPGVDVELVDP
                     DDDPRYRAYHEAYHELRGRDGITPDMAKTSLRRSNTLIGNMLLRMGDVDALLCGTVGR
                     FDAHFEHVRDVIGLAPGAKVFATMNALMLEKHTLFIADTYVNDDPSAEELAAIAQLAA
                     AEVCRFGLQPKIAMLSHSMFGSSSRPSARKMRAARDLLVQAAPELEVEGEMQGDAALS
                     EDVRRHFLPGTKMHGSANLLIMPTLDAANIAFNLLKITGGYGVTVGPILLGAAKPVHI
                     LNPAATSRRIVNMTALAVADAGALDKLSRE"
     gene            complement(15905..16327)
                     /locus_tag="VM406_14375"
     CDS             complement(15905..16327)
                     /locus_tag="VM406_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HVL77198.1"
                     /translation="MARLAEAPRRSTGLTMLCLAAAAWSLVLPLHAFTLAWTHSIEKI
                     RWEEDYRVEGGLLHLTEARIRGTGAGMEVPEGAVLRNGVWHYRPAVGAVESLRLTRSP
                     YTADYELCWDGACRPMHVMAGPVEETPLVEVFSCAARR"
     gene            complement(16285..18375)
                     /locus_tag="VM406_14380"
     CDS             complement(16285..18375)
                     /locus_tag="VM406_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HVL77199.1"
                     /translation="MSEQYQNVRVPDEAPSVGAEFQEHGLQRKLEGVALKILIAIALA
                     FSSYQLIVAAFHPLSSLVIRSLHVGFLISLTFLLYPALKRGKIDSIPWYDWLLAIGAF
                     ALATYHWIFEVDLIVRAGEPTTADIVVGTTVVILVFEAARRVLGPALPIICAAFLAYG
                     LFGEYLPGTLAHRGYGYDQIIDQLFTGTEGIYGIPTMVSATYIFLFILFGAFLEHAGM
                     INLFNSLALGLVGHAKGGPAKVSVLSSGFMGTISGSGVANVLTVGQFTIPLMKRFGYS
                     PKFAGAVEATSSMGGQIMPPVMGAVAFIMAETLSVPYIEIVKAAAIPAILYYATAFWM
                     VHLEAGRAGLSGLPKDQCPNPWRALKEKWYLILPLIGLVGMLFSGFTPMFAGMTGLAL
                     TAILILGAALAATISNKAFRYVFWVAVGLGAASFKEWGINGIIATIAVLSTACLFFAG
                     GRQTLRAVRMSMVDGAKQALPVGVACALVGVIIGVLTLTGAASSFAGFILEVGQNNLF
                     LSLVLTMLVCIVLGMGIPTIPNYIITSAIAAPALLQLGVPLIVSHMFVFYFGIMADLT
                     PPVALAAFAASSIAKESPMKIGLKAVQIAIAGFVIPYMAVYTPALMLQGEWTVLSVAY
                     ILFKAFLAVGLWGAAAVGYLWAPLNWVERTFAVLSACFLVVAMPITDEIGFALAAAFL
                     AWHVWQKRRAAALA"
     gene            complement(18459..19424)
                     /locus_tag="VM406_14385"
     CDS             complement(18459..19424)
                     /locus_tag="VM406_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HVL77200.1"
                     /translation="MKLGLVRRLLALAAGSALLAAAPVQAQQAQFINVLTGGTSGVYY
                     PLGVALTQIYGKALPGAKSTVQATKASAENLNLLQAGRGEIAFTLGDALSDAWNGNEE
                     AGFKAPLKRLRGVAGIYPNYIQIVASADSGVRTLADLKGKRISVGAPRSGTELNARAI
                     LKAAGMSYSDFAKVEYLPFGESVELMKNRQLDATLQSAGLGVASLRDLATSVRVVVVP
                     IPQDVVQKVGDPAYQAGVIPANTYEGQTAAVPTAYVQNFLVTHEGVPADTVYAMTKSM
                     FENLDQMTAAHAAARSIRLQAATTGMPVPLHPGAERFYKEAGVLK"
     gene            complement(19777..20283)
                     /locus_tag="VM406_14390"
     CDS             complement(19777..20283)
                     /locus_tag="VM406_14390"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HVL77201.1"
                     /translation="MLKPFARYLEHHALWQFNRRAVAGGVALGLFFGILIPFAQILVS
                     AVVAIFVRVNLPVAAFSTLITNPLTFPAVYYLAYQVGGIFLHEEQIATAAAVETHIQY
                     TMAVRDEVLTGWLAPALDWLQTAGLQLIVGLAVLAVVASAVGYVAVNASWRYWVRQRW
                     RKRRLRLA"
     gene            complement(20485..>20965)
                     /locus_tag="VM406_14395"
     CDS             complement(20485..>20965)
                     /locus_tag="VM406_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HVL77202.1"
                     /translation="VADRDGDNQVTSLRTSRGAMLQRAETELVARIEARLAALTRWPA
                     ERGEGLQLLRYEKGNEYRPHFDWFDPALPGPARHLERGGQRLATIVMYLSDVEAGGGT
                     TFPEAGLQIQPRRGHAVFFANTDRFHVPDPRSLHAGEPVERGVKYIATKWLRTTTYA"
BASE COUNT         3617 a         6773 c         6937 g         3638 t
ORIGIN      
        1 gccacgtccg gcacgcggga attcggttcc aggatctgga taaagatctc gccctgcttg
       61 atcattccga gctcgtaacg ggcgcgctcc tcgactgcgg ccgttccttc gcgcaggtcg
      121 cgcacttccg attccagcat cgcgttgcgc gcgcgcagtt catcgttctt gcgctgcgcc
      181 gccgtcacct gtccatccag gtcccacacg cgcagccagc cgcccttccc cagccacaac
      241 gggtactgga tcaggatcag cagggcagca agactcaggg tgatcaggcg catcgtggca
      301 ttctacgcga cgcaaaaacg gccggacagt gccggccgtg ctgcatgcat gcgtgttgca
      361 atcagcgcag attgtagaag gcgttgcggc ccgggtagct ggcgatgtcg ccgaggtctt
      421 cctcgatgcg cagcagctgg ttgtacttcg ccatgcggtc cgagcgcgac atcgagccgg
      481 tcttgatctg cagcgcgttg gtgccgaccg cgatgtcggc aatcgtcgaa tcctcggtct
      541 cgccggagcg atgcgagatg accgccgtgt aaccagcgcg cttggccatc tcgatcgcgg
      601 cgaacgtttc ggtcagcgtg ccgatctggt tgatcttgat caggatcgaa ttcgcaatgt
      661 tcttctggat gccctcgcgc aggatcttgg tgttggtgac gaacaggtcg tcgccgacca
      721 gctgcacctt cttgcccagc gcattggtca gcgtggccca gccgtcccag tcgttctcgg
      781 ccatgccgtc ttcgatcgag atgatcgggt acttgtcgca ccaggtggcc agcaggttgg
      841 tgaagtccgc cgacgacagt tgcaggccct cgccttccag gttgtacttg ccatccttgt
      901 agaactcgga ggcggcgcag tccaggccca gcgcgatctg agtccccggc tcgtagccgg
      961 cctgctcgat cgcctgcagg atcagcttga tcgcttcctc atggttggcg accgacgggg
     1021 cgaaaccgcc ttcatcgccg accgcggtgg tcagcccctt gtcgtgcagg atcttcttca
     1081 gcgtgtggaa gacttcggcg ccgtagcgga tcgcttcgcg gaaagacggc gcgccgaccg
     1141 ggatgatcat gaattcctgg aggtccagat tattgtcggc gtgtgcgccg ccattgatca
     1201 cgttcatcat cggaaccggc atctgcatcg ctgcggaacc gccgaaatag cggtacagcg
     1261 gcagaccgga ttcctcggcg gccgccttgg ccactgccat cgaaacggcc agcatcgcgt
     1321 tggcgcccag acgcgacttg ttgtcggtgc catcgaggtc gagcagcgtg cggtccagga
     1381 aggcctgctc attcgcatcc aggcccatga tggcttccga aatttcggta ttgatgttct
     1441 cgcaggcctt cagcacgccc ttgcccatgt agcggctctt gtcgccgtcg cgcagctcga
     1501 tcgcttcgcg cgagccggtg gaggcgcccg acggcaccgc cgcgcggccc atcacgccgg
     1561 attccagcag cacgtcgcat tccacggtcg ggttgccgcg agagtcgatc acttcacggc
     1621 cgatgatgtc tacgatggca ctcattcttc ttctcccaga tcaaagtaaa ttcgatggcg
     1681 cgcagccgcc gacgccacgc cggcactgca gccggaatca cgcaaagtcg ttttcggcga
     1741 aaccgttctt cttgaccacg cggtcgagtt cgaccagcgt cgccagcagc gccttcatgc
     1801 gcttgagcgg taccgcgttc gggccgtcgg atttcgcctc ggccggattc ggatgggttt
     1861 ccatgaacag gccggcgatg cccactgcga cagccgcgcg cgccagcaca ggcacgaact
     1921 cgcgctgacc gccggatgag gtgccctggc cgcccggcag ctgcacggag tgggtggcgt
     1981 cgaacacgac cgggcagccg gtctcgcgca tgattgccag cgaacgcatg tcggaaacca
     2041 ggttgttgta gccgaacgag acgccgcgct cgcaggccat gaagttgtcg gcgttcaggc
     2101 cggcttcacg cgcagcctcg cgcgccttgt cgatcacgtt cttcatgtcg cctggcgcga
     2161 ggaactggcc cttcttgata ttgaccggct tgccggactg cgcgcaggcg cggatgaaat
     2221 cggtctggcg gcacaggaag gccggcgtct gcagcacgtc gacgacctcg gacaccggct
     2281 cgacttcgtc gatctcgtgc acgtcggtca gcaccggcac gccgatctgg cgcttcactt
     2341 cggccaatat ccccaggccc ttttccatgc ccagaccgcg gaacgacgaa ccggacgagc
     2401 ggttcgcctt gtcgaacgaa gatttgtaga tgaacgggat gccgagcccg gcagtcagtt
     2461 ccttcagggt gccggcggtg tcgagcgcca tttgctgcga ctcgatcacg cagggaccgg
     2521 cgataagaaa aaagggctgg tcgagaccag cctcgaatcc gcaaagcttc acgcagcgat
     2581 ccttctgggt gaggcggcct gcttgtgaac cagcgccgcc ttgatgaatg agatgaacag
     2641 cgggtgaccg tcacgcggag tggacttgaa ctccgggtga tactgcacgc cgacgtacca
     2701 ggggtgagca tcgtcgccgg tgcgcgccag ctccatgatt tcgcacaagc cttcggtcgg
     2761 ggtacgtgcc gacacaatca agccggcttc ttccacgcgc ggcagatagt ggttgttcgc
     2821 ttcataacgg tgacggtgac gctcggtcac cttgtcaccg tagatttccg ccgcaatggt
     2881 gccgggtttt accgcgcagg tctgcgcgcc gaggcgcatc gtgccgccga gatcggaact
     2941 ggcgttgcga cgctcgacct ttccgtcgtg gttctgccat tcggtgatca gcgcgacgac
     3001 cggatgttcg gtatcgggtt cgaactcggt ggagttcgcg ttcttcaggc ccgccttgtt
     3061 gcgcgcgtac tcgatcagcg cgacctgcat gccgaggcag atacccaggt acggcacctt
     3121 ttgctcgcgc gcatacttcg ccgcggcgat cttgccctcc acgccgcgct tgccgaagcc
     3181 gcccggcacg agaatcgcat cgtacttctc gagcatgctg gtgccgcgca cctcgatctc
     3241 ctcggaatcg atgtactcga tgttgacgcg gctttcggta tggatgccgg cgtgacgcag
     3301 cgcctcggtc agcgacttgt acgattcggt caggtccaca tacttgccga ccatgccgat
     3361 cgtgacatcc tgcttcggat gctcgagcgc gtagacgagc ttgttccaga ccgacaggtc
     3421 ggccggcggc gcgtcgatgc cgagcttctc gcacacgatg cgatccaggc cctggtcgtg
     3481 cagcatctgc ggaatcttgt agatggtgtc cgcgtcccag acggaaatga cggcgtcctc
     3541 gcgcacgttg gagaacagcg agatcttcgc gcgctcgtcg tcgggaattc ggcggtcggc
     3601 gcggcacagc agcgcgtccg gcgaaatgcc gatttcgcgc agcttctgca cgctgtgctg
     3661 cgtcggcttg gtcttgagct cgcccgcgga agcaatgtat ggaaccaacg tcaggtgcac
     3721 gaaggcggtt tcttggcgtc cggcgcgcag gctcagctga cgtgccgctt ccaggaacgg
     3781 cagcgattcg atgtcgccga ccgtgccgcc gatttcgacc agcgcgacat cgaagccttc
     3841 ggcgccgcga tggatgaaat cctggatctc gttcgtgatg tgcggaatca cctgcaccgt
     3901 cttgcccaga tactcgccac gccgttcctt gcggatcacc gattcgtaga tctggccggt
     3961 agtgaagttg ttgagcttct tcatcttggc ggtgatgaag cgctcgtagt ggccgagatc
     4021 caggtcggtc tcggcgccat cgtcggtgac gaacacttcg ccgtgctgga acgggctcat
     4081 cgtgccggga tcgacgttga tgtacggatc gagcttgatg agggtgactt tgaggccgcg
     4141 ggattcgagg atcgcagcga gggaggcggc ggcgattccc tttccgaggg aagacacgac
     4201 gccgccagtg acgaatacaa acttggtcat tgcagaggtg tgccggacgg cacttgcggg
     4261 aaattcgaat tataacgtaa tccggtcgcc cggctttccg ccaggcgtgc ttgcatttgc
     4321 gcatttcgca acgaaaaacc gatgcggtgg agctgcggca gcacggacat cagggtgcat
     4381 acagcaactg caggcgaagg taattctggg caccgaccga attcggcagc ccgtgcacct
     4441 cgttggtccg atgcacaacg ccgacgcgca ggccgaacgg cccgtccctc caggcggcgc
     4501 ccagctcgac ttcaccatag acccttttcg ggtcgaccgt atgcgggtca ttgcgggaaa
     4561 acaagcctcc ctgcatggtc gcgttgtaga ccaccaggtt cgcatcggcg cgcaggaatg
     4621 cgtgcagcgt gcgctgtcca ggcaaggcgt caaggcggcc tgcgcgcacc gtgatgccgg
     4681 cgccggcgtc ggtgaagatg ttgccgagcc gcccgcgcag caccggggtc gcatcaagct
     4741 gcctgccgag ctgccagcgc accggcgcgg tttccgcata cagctgcacg ccggcctcgt
     4801 tgcgcacttg acgcgcccaa ccgcgcggct cgggctggtc cagcaggcgg tgcagacggg
     4861 tctgcgtcca ttcgccgccc gcgcagggcc ccaggcagcc gaggtcaagc ccgtagcgga
     4921 catgcgtgcc atcggcgcga tacacctcgt ggaaaacccc gccgtacaac cagccggcgt
     4981 acggccggtc cggcgggccg accaaggccg gcggcaaatt gatattggat ggcgtgtaca
     5041 gctcctgtcc gagccgccag ccggccaccc gcacctcgct tgcgctcgcc gtgctgcgcc
     5101 tgtatgcgta gcgcacgccg ctggtgtaaa agccgtcgcg ctgcgtgagc agcagcgagt
     5161 cgttgtcgat gtcgacctgg tggctggccg taccgtgggc gtagacctgg cgatagtcgt
     5221 cgaccagtgt tcccgccgcg accgggagcg tgatcgcggc aagaaggcct gccgcgctgc
     5281 tgcgtaggat gttgttcatg gcttgttcag cagggaatgg atcatcttgt ggacggcctt
     5341 ggcggtttcg gccggggctt ccatcgggaa caggtgaccg cccggcacct gcatgaaatg
     5401 acgtccgacc agccggcgtg tggcggcaag cccggcctgt cggcactcga cggaatcgtg
     5461 cccgccgatg aaaccgaccg gaacaggata ggagcgcgag gccagcttgc cgagatggtg
     5521 cggcaggctg cgatagatcg cggtttccgt ttcgcgcgaa aagcgcagcg tcacgccttc
     5581 gggatgcggc accaggccgt gcttcagata gtcacgcagc acccgccggt cccagcgggc
     5641 gaagacatcc ttggacgcga aatgacgcca ggcggcctcg gcatcgggcc agacattgcg
     5701 gcgtcgctct gacaagcgcg caggcgaaaa gcggtcggcc agcttcagcg gcttgatcgc
     5761 gccccagatt gccgcgcgcc agcctgccac taccggcgaa tcgagcagga ccaccgcccg
     5821 caccagttcc ggccgcgcct tggccgccat caggcttagc atgccaccga gggagtgccc
     5881 gaccagcacc accggctcgc gatagcgctt gtgcaactcg tcggccagtt cacgctccag
     5941 ccaggaccag ccgttggtga cggggtaagc ggggttgtgc gcatgcatgt cgagcgcatg
     6001 cacgtcgtaa tgcttcttca tctgctcgaa caacaccgtg taggttccgg ccgggtaact
     6061 gttcgcgtgg gaaaaatgga tggtgatgcg tttcatggga ttcgttgacg attcggccgc
     6121 gagattctac cgaggccgcg ccgtctgcgt cttgagggtt cgctccagac gcggccccct
     6181 caccgcatca acgctgccgc cagtaccgcg catgctcctg cctataggca ctgaccgcaa
     6241 cttcctcgcc gaagcgcagc gtgaccgcgc cggtctcgtc ggtgcgcagg cgttcggctc
     6301 caagctcgcc gtagcggcgg tagatctcat ccttaggatg ctggtaacgg ttcgcacggc
     6361 cgacctggaa cagcgcaatc tccggctgca ccgcctgcag gaacggcagc gtcgacgagg
     6421 tgccgctgcc gtggtgcggg gcgagcaaca cgtgagcgcg caggcgttcg ggtccggctg
     6481 aaagcagatg gccttcctgt accgcctcga tgtcgccggg aagcaggatc gcatgccgtc
     6541 cgagcgtgac cttcagcgtg cagctgcgcg cattcggctt ccatttggca ctctcgtaga
     6601 ccgccgcgac ggggtacagc atttcgaact gcacgccgtc ccattcccag gcctggcccg
     6661 cctcgcagcg gctgtgttcg cgcgccgcct gcacgatgcg atggccggga tcgagcgagg
     6721 atgacaccca gccgacgtcg atttcgccca ggatggacag cgccccgccg gaatgatcga
     6781 catcgctgtg cgaaatcacg atcgcgtcca gcgcgtggat cccgcgccct ttcagatagg
     6841 gcaggatcac gcggttgccg ccgtcggatt cgggcgagta cgcggggccg gtgtcgtaca
     6901 gcaggcgccg cgtcggcgtc tccaccagca gtgccatccc ctgcccgaca tcgaaggccg
     6961 tgacccacat ctccccggcg cgcggcgccg ccgcgccagg cagcatcagc ggcaccatgc
     7021 aggccacgcc caacatgcgc accggccagc cgcgcggggc cagcagccag accgcgccga
     7081 gcgcggcaat tgcgaacacc caccaggacg gctccggcgc cgtccagacc gcctgcggca
     7141 gcgcgctcat ccagtgcagg acccgggcaa ggccaagtac cagccagtgc gccgcgccca
     7201 gcacccattc ggcagccggc atcggcagga cgctgccggt aagcgcggcc ggcgtaacca
     7261 ggaaactgac cagcggaatc gcgaccgcat tggcgagcgg actcaccagc gacacctggg
     7321 caaacagcag catcgtcagc ggcaccagcg cgagcgtcac cgcgtactgc acctgggccg
     7381 cgccgcgcag gttgtgcaag gtcctttgcc accatccccg ccctgcttcg ccggtggtcg
     7441 cacgtccggc cgtgctgtag aggatcgccg cgaccgcgcc gaacgacagc cagaagccgg
     7501 gttgcagcac cgcccaggga tcgagcagca ccactacgcc aagcgcgatg cacagcacat
     7561 gcgagatggc ggtgatgcgc ccgaaccaca gcgccgccgc caccacgctc agcatggtga
     7621 gcgtgcgttg cgcggggatg ccgaagccgg cgagcagcac gtagagcagc gcggcgccgg
     7681 cgccggccag cgcggcggcc ttgcgcgccg gaagcagcaa cggcagctgc gcgccggtga
     7741 agaacgagcg gcgccacagc gcgagcacga gcgaggcgaa cagcgccgag atcatcgtga
     7801 tgtgcagccc ggagatcgag acgaggtggc tgatgccggt gcggttgaag accgtccagt
     7861 cggactgggc aatcgcgcgc tggtcgccga tcacgagcgc aacgatgacg ccggcgtact
     7921 cgtgctcggg cagcgcccgg tagatgcgct cgcgcatcca cgcgcgggcg cgctgcacga
     7981 ctgcgttgaa gccgggaacg aaagcgtcga ggcgaaggtt cttctgttcc agccgctggt
     8041 cgggacgcac atagccggtc gcacgcagct tctgctcgag cagccagact tcgtagtcga
     8101 agccgtgcgg attcgcgttg ccgtgcgggg gacgcaggcg tgtcacgagc tgccagcgtt
     8161 cgcccggctg cacctcgggc accgcctgcc gctcggccgg tcgaaagccc gcgtaccagg
     8221 aaagggcgac gcgagccgga aagccggccg gcagcggctg cccatcgacc tgttcgaccg
     8281 ccaggttgaa gcggatgccg cggtcgaagc ggtatggcag gctgtccacc gtgcccacga
     8341 gcacgacatc cgttccctcc cactgcggcg cgagcgcgcg cgccaggtgc gcatgcgcga
     8401 agaggccggc ccaggcaaac ccggcaatga tgccgcaggc tgcgaacgaa accacccgaa
     8461 cctgcaccgg ggcgcgccgt gccagccagg cgccggcgag cgcgagcagc aatgtgccaa
     8521 tgaggatgaa aacgggaaga agctcggcct gccgctgcag caccgccgcg ccggcggcaa
     8581 aaccgacaag cgcggcgcgc atgctacaaa ccgacgatcc gcggcagcgc ggccaaggcg
     8641 ttggccgtgg tcgcttcggc gatgcggccg gcatcgctgc cgcgcaactc ggcgagaacc
     8701 gcgccgatgc gcggcaactg ttccgggctg ttgcggccgg gatggatcca ggcgggcggg
     8761 atgtcgggcg cgtcagtttc cagcaccagt gcgtccagcg gcaactgccc ggccaggcgg
     8821 cggatctgca gcgcacgggg aaaagtcatc gcgccgccga agcccagctt gaagccaagg
     8881 cggatgaact cctccgcctg ctgaaagctg ccgttgaacg catgcgcgat gcccttgcgc
     8941 acctcggtac gccgcaggta tttgaggatg atgtcctgcg agcggcgcac atgcagaagc
     9001 accggcagac cgaagtcgcg cgcgatcttc agttgctcgc tgtagaagcg ctcctgcttt
     9061 tcacgcattg ggccctgtgc cagtcctggg atgaagaaat ccagtccgat ttcgccgacc
     9121 gcgacaaaac gcggatcatc catggcggcc tgcacggccg cgcgcagccg gtccaggtca
     9181 tcgtcgttcg cctgcggcac gaagatcgga tgtatgccca gcgcatagct gcagttgggg
     9241 ttctccgcgg cgagccgccg cacggcatcg aagttgacga cgcccacggc gggaatcacg
     9301 atctgccgca cgccctggat cgcggcccgg gccgccactt cggcgaattc gccttcgaac
     9361 tcgctcgcat ccagatggca gtgggtatcg atccacatct attcgctata cggatgcagg
     9421 ccggcttcgg tcaggcgcag cacccggtcg cagtggcggg caagttcgat gtcatgggtg
     9481 acgatcacga aggcggtgcc gagcgtgcgc gacagctcca gcatcaggtc gaaggtcttc
     9541 tgggcggtga cccggtccag gttgccggtc ggctcgtcgg ccagcacgca ggccggctgc
     9601 gtgaccagcg cacgcgccag cgcgacacgc tggcgctcac cgccggacag ttcgccgggc
     9661 acatgggtga cgcgcgcggc cagaccgacg cgggcgagga tcttgcgcgc ctcgtcatgc
     9721 gcagcgctgc gcgacatgcg ccggatcatc agcggcatcg cgacattgtc gagcgcggag
     9781 aattccggca gcagatggtg gaactggtag acgaagccaa gcgaacgatt gcgcacctcg
     9841 ccgcgctgct tctcgctcag gcgcgccagt tcctggccaa gcaggctgac cgtgccggct
     9901 gtcggcaggt cgagcccgcc cagcagatgc agcagcgtgg acttgccgga accggaggcg
     9961 ccgacgatgg cgacgcgctc gccgcggtag acggaaaagt cgatgccgcg caggacctcg
    10021 actgaatatt tgccttgcgt gaaggtcttg cccagcgcct ggcaggacag aacgggttga
    10081 tccatggttg tcactcgtag cgcagggctt cggccggccg cacgttggcg gcgcgccagc
    10141 tcggataaag ggtcgcggca aaggcgagca ggaccgcgat gccactgatc gttccgacat
    10201 cggaccagcg caggtccgac ggcagctcgg tgatcaggta gatgtccttg tcgaggaact
    10261 gcacgccgag cacacgctcg atgaaaggca cgatcacgtc gatgttctgc gcaaccagca
    10321 cgccggcgct cgcgcccagt gcggtgccga gcaggccgac cagcgccccc tggatgatga
    10381 aaatcttcat gatcgaaccg ggcgccgcgc cgagcgtgcg caggattgcg atgtcggcct
    10441 gcttgtcggt gaccgtcatc accagcgagg acaccaggtt gaacgcggcc accgcgatga
    10501 tcagcgtgag aatgatgaac atcatgcgct tttccacctg caccgcggcg aaccaggtgc
    10561 gattctgacg cgaccagtcg aggatcagca ggttgccggt cagcgtgcgc gaaagctcga
    10621 gcgcaacctg cggcgcacga tgcatgtcgg caatgcgcag gcgcaggccg gtcggccccg
    10681 tctgctggaa catgcgcgcc gcgtcgtcga tatggatgaa ggccaggccc gcgtcgtact
    10741 cgttgtggcc ggcgcggaag atgccggcga ccgtgaactg gcgcaggcgc ggcagtacgc
    10801 cggccggcgt cacctgccct tgcggtgcga tcatcgtcac gcggtcgccg acgtgcacgc
    10861 cgaggccgcg ggccagctcg ctgcccagaa cgacgttgaa ctcccctggc tgcagcgccg
    10921 cgacactgcc ttcgacgacc tgatccgcca cgtccgacac cttgggctcc tgctccggca
    10981 ggatgccgcg gatcacggtg ccgcgtacgc tgtcgccgcg ggtgagcatg gcctgggcgg
    11041 cgatgtacgg agcggcgccg atcacctccc gatgacgcag cgtttcctcg gcggtcgcct
    11101 gccagtccgg caggctgccg taggcgtcga acacttcgat atgcgagagg accgacagca
    11161 tgcggtcacg cacctcggtc tggaaaccgt tcattaccga gatcacgatg atcagcgcgg
    11221 ccacaccaag ggcgatgccg aacatcgaaa tcagggagat gaaggagatg aagctgttgc
    11281 gcccgctgcg gcgtccggcg cgcgtgtagc gcagcccgac cagccactcg aacgggagat
    11341 tcctggtgaa acgcaaaaga tggctcctgg agtgatgcgc agacaataac ggaatagcaa
    11401 cttcttgcgc agcaggcagt ttgccataca aaaaggtttg ccgggcggtg ctttggtcaa
    11461 gaaggcgggt ggccggaccc gtacgcgcgc aagcgaacgc gggacgcacg tcccgaggtg
    11521 ttgcagcgtt cctgccggcg ttttagtgcc gctgcagcat gcggtccatg cgttccagca
    11581 tgcccttgcg gttcttgtgc ttgacttcgt agctgcgcaa cttgcggatg tccgcagccg
    11641 acagcttttc catttccttg ctgatctgtg cgatcgtcat ctgctggtag ccttcgatcg
    11701 gcaggccgcg gtcgggcgat ctgccctgct tgtccttctg cttttcggtg cgggtttcgc
    11761 cgtactggct gcgctgcttg ttgacgatgc gcgaggccac ctcctcctcg cggcccggat
    11821 agcgatgctc ctgcttgaag cgcttttcca gcttcttgta ctcgcgctca cgcttcggac
    11881 tggctcctgc tggcatggcg gtctcctgtt gcaacatggc cgatcctgct gtgagcccgc
    11941 caatgcgcgc cggttcccgc ggttctcatt tggtgcgttt tgtgcttgtg gcaagacaag
    12001 acgtgaaagg agcccggatg cgactattca aacttgatca cctggtcagc atgatcgccg
    12061 cacccatcat ctgggcggca cacttcctca tctgctacac gatggtctct ctcgtttgca
    12121 tggtcggctg gacgcatgcc cggctgttcg gcatgaatcc cggccagatc gccgtcgcgc
    12181 tggctaccgt ggtcggagtc gtcctgctcg gctacaccgg cgtggtgaat ctcgaaaaat
    12241 accggcaatc ccgcaaagac gaaaccggca ccatcgaagt cagcggcttc gttgcgctca
    12301 acgcgctctt gctgtccatc ctttccaccg tggccgtggt ctgggtggcg tttccgacac
    12361 tgatgctgcc cacatgcgcg atctgaacat gaagctggcg ctgctcgccg ccgccctctt
    12421 tccggctagc gcccacgcgc acggtgaacc cggcgcgcat cactggtgga cgctggaccc
    12481 gtggatcacg attccgcttg caggctggct gctcctttat gcacgcggcg tgcaactttt
    12541 acgcgatcac aaccgccgca ccggccgcag cctgagagtc gtgcaggcgc cggcgctggc
    12601 tgcagccgcg cttggcttcg cgtcactgtt tctcgcgctc atctggccgc tcgacgcgct
    12661 gagcgccgac agtttcgccg ctcacatggc gcagcatatg gtcttgatcg cgctcgccgc
    12721 accgctgctg gtgtatgcgg agccgtcggt tccggtcacg cgcgcgcttc ctgccttgcg
    12781 cgtcgtcggc cgctcgcgcg tattgaaccg gctgctgcgc atcggcctga agccccgcgt
    12841 ggcctttgcg gtgcacggca ccgtgatctg gttctggcac gcgccgacgg tattcgaact
    12901 ggcggtacgc aacgatttcg tccatgcgct cggccacatt gcattcttcg gcagtgcgct
    12961 gctgttctgg gcctcgctgc ggcaggtggg ccgcgcgggg ggcgacggct acggcgtggc
    13021 cgccctgtgg acgctgggca cgatgatgca tatgggtttt ctcggcggga tcatcacgtt
    13081 tgcgccgcgc gtgctctatg acgtgtacgc cgacggcggc atcggcggct tgtcgccaat
    13141 ggaggatcag cagctggccg ggctgatcat gtgggtgccg gccgcgcttc cctacctggt
    13201 ggcgggcatg ggctttatcc tggcgtggct gcgtgacgcg gaccggcacg ctccgcgggt
    13261 gcaatggcgc cgggactgac tggaagaacg ggcggtgccg cgatggcacc gcccttggcc
    13321 tcactcgcgc gagagcttgt cgagcgcccc ggcgtccgcc accgccagcg ccgtcatgtt
    13381 gacgatgcgg cgcgaggtcg cggccgggtt caggatgtgc accggtttgg ccgcgcctag
    13441 caggatcggt ccgaccgtca cgccgtagcc gccggtgatc ttcagcaggt tgaacgcgat
    13501 gttggccgca tccagcgtcg gcatgatcag caggttggcg ctgccatgca tcttcgttcc
    13561 gggcaggaag tgacggcgaa cgtcttcgga cagcgcggca tcgccctgca tctcgccttc
    13621 gacttcaagc tcgggcgcgg cctggaccag caggtcgcgc gcggcgcgca tcttgcgcgc
    13681 cgaaggccga gatgaggaac cgaacatcga gtgcgacagc atcgcaatct tcggctgcag
    13741 gccgaaacga cacacctcgg ctgcagcaag ctgggcgata gccgcgagtt cctccgcgct
    13801 cggatcgtcg ttcacatagg tatcggcgat gaacagcgtg tgcttttcca gcatcagcgc
    13861 gttcatcgtc gcaaacacct tcgcgcctgg cgcgaggccg atcacgtcac ggacatgctc
    13921 gaagtgggca tcgaagcgcc cgaccgtgcc gcacagcagc gcgtcgacat cgcccatgcg
    13981 caacagcatg ttgccgatca gcgtgttgga gcggcgcagc gacgtcttgg ccatgtcggg
    14041 cgtgatgccg tcgcgtccgc gcagctcgtg gtaggcctcg tggtaggcgc ggtaacgcgg
    14101 gtcgtcatcc ggatcgacca gttcgacatc cacgccgggc ttcagccgca ggcccgcttt
    14161 ctggatgcgc atctcgatca catgcggacg gccgatcagc accggcttgg ccagcccttc
    14221 gtcgaccacc gtctgcgcgg cgcgcagcac gcgctcctcc tcgccttccg cataggcgac
    14281 gcgcttgggc gccgacttcg cagccgagaa caccggcttc atgatcatgc cggtgtgata
    14341 gacgagctgg gagagctgct ggcgataggc gtccaggttc tggatcggcc gcgtcgccac
    14401 gccggattcg gccgccgcct tggccactgc cggcgcgatt ttctcgatca ggcgctggtc
    14461 aaacggcttg ggaatgatgt actcggggcc gaacgtcagg tcctggccgg cataggcctg
    14521 cgcgacagcg tcgttggtct ccgcctcggc cagttccgca atcgccttca cgcaggcgag
    14581 cttcatctcg tcggtgatct tggtggcgcc gcagtccagc gctccgcgga aaatgtacgg
    14641 gaagcacagg acgttgttga cctgattcgg atagtcggat cggccggtgg caatgatgca
    14701 gtccggacgc gcggccttcg cgacttccgg gcggatctcc ggctccggat tggcaagcgc
    14761 gaggatgatc gggcgatcgg ccatggtctt gaccatctcg ccggtcagca cgcctgcggt
    14821 cgagcagccg aggaagacgt cggcgccggc cacgatgtcg gccagcgtgc gcgcgttcgt
    14881 gtcctgcgca tagcgcgcct tgttctcctc catcttggcg tcgcgcccga tccagatcac
    14941 gccgcgcgaa tcggcaacga agatgttctc gcgccgcacg cccaggctga ccatcaggtc
    15001 caggcaggcg atcgccgcgg cgccggcgcc ggaggcgacc agcttgatct cttcgatctt
    15061 cttgtcgacc accttgagac cgttgagcaa tgcggcggcg gaaatgatcg cggtgccgtg
    15121 ctggtcgtca tggaagaccg ggatgttcat gcgctctcgc aactgcttct cgatgtagaa
    15181 gcattccggc gccttgatgt cttccagatt gatgccgccc accgtcggct cgagcatcgc
    15241 gatcgcctcg accagcttgt ccgggtcgtg ctcggcgagt tcgatgtcga atacgtcgat
    15301 cccggcgaat ttcttgaaca ggcagccctt gccttccatc accggcttgc cggccagcgg
    15361 gccgatgtcg ccgagtccca gcaccgcggt gccgttggtg atgacggcca ccaggttgcc
    15421 gcgcgaggtg tactcggcgg ccatcgccgg gtccttcgcg atttcctcgc aggcataggc
    15481 gacaccgggg gagtacgcga gcgacaggtc gcgctggttg gaaagcgcct tggtggggtt
    15541 gacttcgatc ttgcccctgg tcgggctgcg gtgatattcg agcgcggctt cgcgcagttg
    15601 gttgtccttg cctagctggc tttcggtagt catggtcttt tattcgtcaa tgagaggcac
    15661 gcggcgatgc ctgcgaaagt tgcgtgcaga aattgccagt tgcctgccat accggacgcc
    15721 gtggctcctt gtgagagcgg cgaggcggca cggcgttcgg aatgacaggc aggtcgtcgg
    15781 accggggatg tggccgaact ggcattgtaa cgggatggcg cgccgtcgga gcggagcgcg
    15841 gcaatgcgaa gccgccgggc gcccatgcgc aatgctgaag acggccttct attgagcagg
    15901 acgatcagcg cctggccgcg cacgaaaaga cctcgaccag cggcgtctct tccaccggtc
    15961 ccgccatcac atgcatgggc cggcatgcgc cgtcccagca aagttcgtaa tcggcggtat
    16021 aaggagaccg tgtcagccgc agcgactcga cggcaccgac ggccggtcgg taatgccaga
    16081 cgccattgcg cagcaccgcg ccttcaggca cctccatgcc cgccccggtg ccgcggatgc
    16141 gtgcctcggt cagatgcagc aagccgccct cgacccggta gtcctcttcc cagcggatct
    16201 tttcgatgga gtgggtccag gccagcgtga aggcgtgcag cggcagcacg agactccatg
    16261 ccgccgccgc aagacacagc atcgtcaggc cagtgctgcg gcgcggcgct tctgccagac
    16321 gtgccacgcg aggaacgccg cggccagcgc gaagccgatt tcatcggtga tcggcatcgc
    16381 gaccaccagg aagcaggcgg acagcacggc gaacgtgcgt tccacccagt tcagcggcgc
    16441 ccacaggtag ccgactgccg ccgcgcccca caggccgacg gcgaggaagg ccttgaacag
    16501 gatgtaggcg accgacagca cggtccattc accctgcagc atcagcgctg gcgtgtacac
    16561 ggccatgtac ggaatcacga agccggcgat cgcgatctgc accgccttca ggccgatctt
    16621 catcggcgat tccttcgcga tcgaagacgc cgcgaacgcg gccagcgcga ccggcggcgt
    16681 caggtcggcc atgatcccga aatagaacac gaacatgtgc gagacgatca gcggcacgcc
    16741 gagctgcagc agcgccggcg cggcgatcgc gctggtgatg atgtagttcg gaatggtcgg
    16801 aatgcccatg cccagcacga tacagaccag catggtcagc accagcgaca ggaacaggtt
    16861 gttctggccc acctcgagaa tgaagccggc gaagctcgat gcggcgccgg tgagcgtgag
    16921 cacgccgatg atcacgccaa ccagcgcgca ggccacgccg accggaagcg cctgcttggc
    16981 gccgtcgacc atcgacatgc ggaccgcgcg cagcgtctgg cgaccgccgg cgaagaacag
    17041 gcatgccgtc gaaagcaccg cgatggttgc gatgatgccg ttgatgcccc attccttgaa
    17101 gctcgcggcg cccaggccga cggccaccca gaacacatag cggaaggcct tgttcgagat
    17161 cgtggcggcc agtgcggcgc cgaggatcag gatcgccgtc agcgcaagac cggtcatgcc
    17221 ggcgaacatc ggggtgaaac cggagaacag catgccgacc aggccgatca gcggcaggat
    17281 caggtaccac ttttccttca gcgctcgcca cgggttcggg cactggtcct tcggcaggcc
    17341 ggacagtccg gcgcgtcccg cttcaaggtg caccatccag aacgctgtcg cgtaatacag
    17401 gatcgctgga atcgccgcgg ccttgacgat ctcgatgtac ggcaccgaca gggtctcggc
    17461 catgatgaac gcaaccgcgc ccatcacagg cggcatgatc tggccgccca tcgacgaggt
    17521 cgcctcgacc gcgccggcga acttcgggct gtagccgaag cgcttcatca gcgggatcgt
    17581 gaactggccg acggtcagca cgttggcaac gccggaaccc gagatcgttc ccatgaagcc
    17641 ggacgacagc accgacacct tggccgggcc gcccttcgca tggccgacca ggcccagcgc
    17701 cagcgagttg aacaggttga tcatcccggc gtgctcgagg aaggcaccga acaggatgaa
    17761 caggaagatg taggttgccg acaccatcgt cgggatgccg tagatccctt cggtgccggt
    17821 aaacagctgg tcgatgatct ggtcgtagcc gtagccgcga tgtgcgagtg tccccgggag
    17881 gtactcgccg aacaggccgt atgccagaaa ggctgcgcag atgatcggca gcgccggccc
    17941 gagcacgcgg cgcgcagcct cgaatacgag gatgacgacc gtggtgccga ccacgatgtc
    18001 ggcggtggtg ggttcaccag cgcgcacgat caggtcaacc tcgaaaatcc agtgataagt
    18061 ggccagcgca aacgcgccga tggcgagcag ccagtcatac cacggtatgg agtcgatctt
    18121 gccgcgcttt agcgcggggt acagcagaaa ggtcaggctg atcagaaagc ccacgtgcag
    18181 cgagcggatc accaggctgg agagcgggtg gaacgccgcg acgatcaact gatagctgga
    18241 gaaagcgagc gcaatggcga tcaagatctt cagcgcgacg ccttcgagct tgcgctgcaa
    18301 gccatgctcc tggaattccg cgcccacgga cggtgcttcg tccggcactc ggacgttctg
    18361 atactgttcg gacatgaaac tgctcctgat ttggaattgg cgcgggtcgg aaggctccgc
    18421 ccgcgctctt ttcttttcgg tgctaccgat tcaagcgctt acttgaggac gcccgcttcc
    18481 ttgtagaagc gctcggcacc ggggtgcagc ggcaccggca tgccggtggt ggcagcctgc
    18541 aggcggatcg agcgtgcggc tgcgtgcgct gccgtcatct ggtcgaggtt ctcgaacatc
    18601 gacttggtca tcgcgtagac cgtgtcggcc ggcacgcctt cgtgggtgac caggaagttc
    18661 tgcacatagg cggtcggcac ggcggcggtc tggccttcat aggtgttggc agggatcacg
    18721 ccagcctggt aggccgggtc gccgaccttc tggaccacgt cctgcgggat cggcaccacg
    18781 accacgcgca ccgaggttgc caggtcgcgc agcgaggcca cgcccaggcc ggcggactgc
    18841 agggtcgcgt cgagctggcg gttcttcatc agttcgaccg actcgccgaa cggcaggtac
    18901 tcgaccttgg cgaaatcgct gtagctcatg ccggcggcct tgaggatcgc acgtgcgttg
    18961 agctcggtgc ccgagcgcgg cgcgccgacg gagatgcgct tgcccttcag gtcggccagc
    19021 gtgcgcacgc cggagtcggc ggaggcgacg atctggatgt agttcgggta gatgcccgcc
    19081 acgccgcgca ggcgcttgag cggcgccttg aagccggctt cctcgttgcc gttccaggcg
    19141 tccgacagtg cgtcgccaag ggtgaacgcg atctcaccgc gaccagcctg cagcaggttc
    19201 aggttttctg cagaagcctt ggtggcctgg acggtgctct ttgcgcccgg cagcgccttg
    19261 ccgtagatct gcgtcaaggc cacgccaagc gggtagtaga cgccgctggt gccgccggtc
    19321 aaaacgttga tgaattgcgc ttgttgcgcc tgtacggggg ctgctgcgag cagtgcggaa
    19381 ccagcggcaa gtgccagcag gcgacgaacg agtccgagct tcatgtttga tctcctcgag
    19441 tggttttata gataccggcc ggggtcggcg gcagaaacgc atgaagcgcg tccgcaatgg
    19501 tgacgcgctt caaacgttcc gatgatgcca tattccctac gaggcggcat tacggaatat
    19561 tgcttattgt caataagccg gataccgcgt ctcgcgcagg cagttagcat cccgcatgcc
    19621 aaccgacaac ctgcgggcaa gccgttgaac aggaagggat ttttcgggga gcgctggagc
    19681 gcgggagcag caggcggatg gaagaccgca ctgaggaagg ggccggtgtg cggatatccg
    19741 gaagcgggac ggcggtcgac gccatcccgc cgggcctcag gcgaggcgca ggcggcgctt
    19801 gcgccagcgc tgccggaccc agtagcgcca ggaggcattg accgcgacgt agccgaccgc
    19861 ggaggccacg accgcgagca cggcaaggcc gacgatcagc tgcaggccgg cggtttgcag
    19921 ccagtccagc gcgggcgcga gccagccggt cagaacctcg tcgcgcaccg ccatcgtgta
    19981 ctggatgtgc gtctcgacgg ccgccgccgt ggcgatctgc tcctcgtgca ggaatatgcc
    20041 gccgacctgg taggccaggt agtagacggc cgggaacgtg agcggattgg tgatcagcgt
    20101 cgagaacgcg gccacgggca ggttcacgcg aacgaagatg gcgaccactg ccgacaccag
    20161 aatctgcgcg aacgggatca gaatgccgaa gaacaggccg agcgccacgc cgccggccac
    20221 cgcccggcgg ttgaactgcc aaagcgcatg atgttcgagg tagcgcgcga acggcttgag
    20281 catgcggctt tgcagcagcg cttgcggctg gggcagacgt tgaaagatgg gatgccgggg
    20341 catgatggga tgaggactcg ctacgcgcaa acggggcgcg ctggcaaggc attttacaga
    20401 tcgaaaggcc tcgcgaaagg aaatattttt gtgcggcgtc aagccgtacg gtgacgaacc
    20461 agcaaggccc gcaggcgtgc gcgctcaggc ataggtggtg gtgcgcaacc acttggtcgc
    20521 gatgtacttc acgccgcgtt caaccggttc gccggcatgc agcgagcgcg gatccggcac
    20581 atggaagcgg tcggtattgg cgaagaacac cgcatgtccg cgccggggct ggatctgcag
    20641 gccggcttcc gggaaggtgg tgccgccgcc ggcttccacg tcgctcaagt acatgacgat
    20701 ggtggccagg cgctggccgc cacgctccag atgacgcgcc ggccccggca gggccgggtc
    20761 gaaccagtcg aaatggggac ggtattcatt gcctttttca tagcgcagca gctgcaggcc
    20821 ctcgccccgc tcggccggcc agcgggtcag cgccgccagg cgcgcctcga tacgcgcgac
    20881 cagctccgtc tccgcgcgct gcagcatcgc accgcggctg gtacgcaggc tggtcacctg
    20941 gttgtcgccg tcgcggtcgg ccacc
//
LOCUS       DATLGF010000464         4945 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_216847, whole genome shotgun sequence.
ACCESSION   DATLGF010000464 DATLGF010000000
VERSION     DATLGF010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216847"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VM406_14400"
     CDS             complement(<1..442)
                     /locus_tag="VM406_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HVL77203.1"
                     /translation="MTTLRTGGQILVDALQTQNVEIAFGVPGESYLAVLDALHDSPIR
                     FIVNRQEGGAAFMAEAYGKLTGKPGVCFVTRGPGATNAAIGVHTAHQDSTPMVLFIGQ
                     VGNDFVEREAFQEIDYRRMFGQMTKWVAQIDRADRVPEYVARAFQ"
     gene            539..894
                     /gene="rnpB"
                     /locus_tag="VM406_14405"
     ncRNA           539..894
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VM406_14405"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1670..2098
                     /gene="mraZ"
                     /locus_tag="VM406_14410"
     CDS             1670..2098
                     /gene="mraZ"
                     /locus_tag="VM406_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HVL77204.1"
                     /translation="MFQGASALNLDAKGRMTIPARHRDALTVQCEGRVTLTKHPHGCL
                     LLFPRPVWENHREQIAAWPMSARAWQRIFLGNASDVEMDGAGRILIAPELRSAVGLTR
                     DVMVLGMGSHFEIWDAAKLAESEAEAVAAGMPDVLNNFSF"
     gene            2102..3067
                     /gene="rsmH"
                     /locus_tag="VM406_14415"
     CDS             2102..3067
                     /gene="rsmH"
                     /locus_tag="VM406_14415"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HVL77205.1"
                     /translation="MTTDKAVPELQHRTVLLQEAVDALAIDGARADGVYVDGTFGRGG
                     HSRLILERLSERGRLVAFDKDPQAIAAAQALGDARVAIVHDSFATMAAALAERGVGQV
                     DGVLLDLGISSPQVDDAARGFSFRQDGPLDMRMDPTRGMSAAQWLATADEQTIGKVIK
                     EYGEERFAFQIAKAIAARRAVEPISGTRQLAEIVAHAVKTREKGKDPATRTFQAIRIY
                     INKELEELEIGLDAAFRALGPNGRLAVISFHSLEDRIVKRFLAGKAKVAQPDRRLPIR
                     AAELPQPELKLLAKIKPSDAEIAANPRARSAILRVAERLPAGGRQ"
     gene            3064..3336
                     /gene="ftsL"
                     /locus_tag="VM406_14420"
     CDS             3064..3336
                     /gene="ftsL"
                     /locus_tag="VM406_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HVL77206.1"
                     /translation="MSWRLNIALTGALVGCALLLVNAQYQSRRLFIELERAQAMSRQL
                     EVEWAQLQLEQSTLGNHGRIEAHARRDLGMARITPDRTQYLTMGGQ"
     gene            3336..>4945
                     /locus_tag="VM406_14425"
     CDS             3336..>4945
                     /locus_tag="VM406_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HVL77207.1"
                     /translation="MAARTAAGRGVAYTSSPVLAVKLPVWRSRVVLFFLFVGFAALAA
                     RALWLQGISTEFLQKQGASRYARTLELPATRGKITDRNGQVLASSLPVRAIWAVPEDV
                     LEAPADKLRELARLLGMSESDLKKRLDVDRTFVYLKRQVEPDVTEQVLKLNISGIGTR
                     REYKRYYPDGGVMAHVVGFTNVEDVGQEGMELAFQKSLAGVTGSRRVIRDRLGRIVED
                     IESVRVPHDGKDLALSIDRKIQYIAYSALKEAVEKHKAKAGGIVVLDAKSGEVLALAN
                     LPTYDPNDRSRLTGAQLRNRVLTDTFEPGSTIKPLTAALAMERGLVRPDTMLQTEGGR
                     FTLGRSTITDTKNYGNMTLSQAIAKSSNVATARMAMQMTPEQMWDLFTSLGFGQAPKL
                     GFPGAVAGRVRPAASWRPIEQVTMSYGYGLSASLIQIAQAYMVFARDGDMIPLTLQKS
                     SDKPIGRRVLSPQTAREMRLMLEGAASPDGTAPRAQVPGYRVGGKTGTAHKLENGRYT
                     NKYIGSFAGIAPISDPRLIVAVMIDEPSG"
BASE COUNT          929 a         1560 c         1632 g          824 t
ORIGIN      
        1 cctggaaggc gcgcgccacg tattcgggca cgcggtcggc gcggtcgatc tgggcgaccc
       61 acttggtcat ctggccgaac atgcggcggt agtcgatttc ctggaatgcc tcgcgctcga
      121 cgaaatcatt gccgacctgc ccgatgaaca gcaccatcgg cgtcgaatcc tggtgcgcgg
      181 tatgcacgcc gatggcggca ttggtcgcgc ccggcccgcg ggtgacgaag cacacgccgg
      241 gcttgccggt cagcttgccg taggcttcgg ccatgaaggc cgcgccgcct tcctggcggt
      301 tcacgatgaa gcggatcgga gagtcatgga gcgcgtcgag gaccgcgaga tagctttcgc
      361 cgggtacgcc gaatgcgatt tcaacgttct gggtctggag ggcgtcaacc aggatctggc
      421 cgccggtgcg cagggttgtc atggtgtctt tccggtttga gaagccgtta cgctaccaga
      481 aaacgcaagg ggacaatttg gtcagcgcgc aggcaggcga tccactagaa tagccgccga
      541 agcaggccag acagccgccg gccgtcgcga gacggcggga ggaaggtccg gactcacaga
      601 gcagggtgac ggctaacggc cgtccgccga aagccgcaag gtataaggcg aggaacaggg
      661 ccacagagac gagtctttgg gaccgcggac gcgagtcagg cggtcacaaa gggtgaaacg
      721 cggtaacctc cacctggagc aattccaaat aggcacgcga tgatgttgct cgcggagcgt
      781 gcgggtagga agcttgagcc gtggagcaat tcacggccta gaagaatggc tgtcatagcg
      841 gtgttggcga caacgccgcc gtaacagaat ccggcctatc ggcctgcttc acgtctttcg
      901 tccgccaagg cagaagttgc gaccatgctc ttgacggact tcaacgccgg ctccttcacc
      961 cccggttaca gtagtggcta ccacccgttg cacccagagc ccgtcggccg tgggatgaga
     1021 aacaggagta gtggaggatt gccggaccgg tttccctgga caacggcggc cgccaactaa
     1081 acgagccggg gcagtcagcc tgctttcccg cttcccgacc ttcatcccac gaccgacggg
     1141 ttctgcgcgt cttgcgcgtc tgcgcgccac tcctcctgcg acttcatcct gcgtgatcgc
     1201 cccgtgaatg cgcggcgcca cagccgcgcc ggcatgtgcg gccctgtcag gcgccgcctg
     1261 cttgtcacgc gtccagcggc ctgcgtcttt ttattccccc ccgcgcattc attccggaac
     1321 gaaattctct tgcgctgcgt caaagcggca gcccaccggc gcacgcccgc cgtgcgggca
     1381 catgaaacac atgtgtacag ctgagagttc accttcagac tcttcgctaa gtgcttcatt
     1441 ctcttcggtt ttgctcttca cgccggctcg aaggaacgcc cgtaagtgct tgactccatt
     1501 gggaaaaatc aaattttcca caaggaatat tacttgacca taaaaaaacc atcctctaaa
     1561 gtgggcggca gtgtgaaaaa gtgtattttt gtggggcaag aagcttggtg ccgcccagaa
     1621 tccacattcc gacaagacaa caaaacggca gccaaacagg ggcgattggg tgtttcaagg
     1681 cgcgtcagca ctgaatctcg atgcgaaggg acggatgacc attccggcca ggcaccgcga
     1741 cgccctgacg gtgcagtgcg aaggacgcgt cacgctgacc aagcacccgc atggctgcct
     1801 gctcctcttt ccgcgcccgg tctgggaaaa ccatcgcgaa cagattgccg cgtggccgat
     1861 gtcggcgcgc gcctggcagc gcatctttct cggcaatgcc tccgacgtgg agatggacgg
     1921 cgccggccgc atcctgatcg cgccggagct gcgctcggcc gtagggttga cgcgagacgt
     1981 gatggtgctc ggcatgggta gccacttcga aatctgggat gcggccaagc ttgccgaaag
     2041 cgaggcggag gcggttgccg ccggcatgcc ggacgtactc aacaattttt cgttctagcc
     2101 cgtgacgaca gacaaagcgg tgccggaact gcagcaccgg acggtgctgt tgcaggaagc
     2161 agtggatgcg ctggccatcg acggcgcgcg cgccgacggc gtgtacgtgg acggcacgtt
     2221 cggccgcggc gggcacagcc ggctgatcct cgagcgcctg tcggagcgtg ggcggctggt
     2281 cgccttcgac aaggatccgc aggcgatcgc ggcggcgcag gcgctgggcg acgcgcgcgt
     2341 ggccatcgtg catgacagct tcgccacgat ggcggcagcg cttgccgagc gcggcgtagg
     2401 acaggtggat ggcgtgctgc tggacctggg tatttcctcg cctcaggtcg acgatgccgc
     2461 gcgcggattc agcttccgcc aggacgggcc gctggacatg cgcatggatc cgacgcgggg
     2521 gatgtcggcg gcgcagtggc tggcgacggc cgatgagcaa acgataggaa aggtcatcaa
     2581 ggaatatggg gaagaacggt ttgcttttca gattgcaaag gcgattgctg ctcgccgggc
     2641 agtcgagcca atttcaggca cacgacagct tgccgagatc gtggcacacg ccgtcaaaac
     2701 ccgcgagaaa ggcaaggacc cggccactcg tacctttcag gctatccgga tttacatcaa
     2761 taaagagctt gaagaactcg aaataggact cgatgcagcg tttcgggcac tgggcccgaa
     2821 cgggaggctc gctgtgatca gctttcattc gcttgaggac aggatcgtca agcgcttcct
     2881 ggccggcaag gccaaggtgg cgcagcccga ccgtcgcctg ccgatccgtg ccgcggaact
     2941 gccgcagccg gagctgaagc tgctcgccaa gatcaagcct tccgacgccg agatcgccgc
     3001 caatccacgc gcgcgctcgg cgatcctgcg cgtggccgag cgcctgcccg ccggaggcag
     3061 gcaatgagct ggcgcctcaa tatcgccctg accggcgcgc tggtcggctg cgcgctgctg
     3121 ctcgtcaacg cgcagtacca gtcgcgccgc ctgttcatcg agctcgagcg ggcgcaggcg
     3181 atgtcgcgcc agctcgaagt cgaatgggcc cagctccagc tcgagcaatc gacccttggc
     3241 aatcacggcc gcatcgaagc gcacgcgcgg cgcgaccttg gcatggcgcg catcacgcct
     3301 gaccgtaccc agtacctgac gatgggaggg cagtgatggc cgcgcgcacg gcagccggac
     3361 gcggcgtcgc ctacacctcc agcccggtac tggcagtcaa gctgccggta tggcgctcgc
     3421 gcgtcgtgct gttcttcctg ttcgtcggct ttgcggcgct ggccgcgcgc gcgctgtggc
     3481 tgcagggcat ctcgaccgaa ttcctgcaga agcagggcgc ctcgcgctat gcgcgcacgc
     3541 tggaattgcc cgccacgcgc ggcaagatca ccgatcgcaa cggacaggtg ctggcttcgt
     3601 cgctgccggt gcgcgcgatc tgggcggtgc cggaggacgt gctggaagcg ccggccgaca
     3661 agctgcgcga actcgcgcgc ctgctcggca tgtccgagag cgatctgaag aaacgcctgg
     3721 acgtggaccg caccttcgtt tatctcaaac gccaggtcga gcccgacgtc acggaacagg
     3781 tgctcaagct gaatatctcc ggcatcggca cgcggcgcga gtacaaacgc tactacccgg
     3841 atggcggagt gatggcgcat gtggtcggct tcaccaatgt ggaagatgtg ggccaggaag
     3901 gcatggagct ggcgttccag aagagcctgg cgggcgtcac cggcagccgg cgcgtgatcc
     3961 gcgaccggct cgggcgcatc gtcgaggaca tcgaatctgt gcgcgtgccg catgacggca
     4021 aggatctcgc gctgtccatc gaccgcaaga tccagtacat cgcctattcg gcgctcaagg
     4081 aagcggtcga gaagcacaag gccaaggccg gcggcatcgt cgtgctcgac gcaaagagcg
     4141 gagaggtgct ggcgcttgcg aacctgccga cctacgaccc gaatgaccgc tcgcgcctga
     4201 ccggcgcgca gctgcgcaac cgcgtgctga ctgacacctt cgagccgggt tccacgatca
     4261 agccgctgac cgcggcactg gcgatggaac gcggactggt tcggcccgac acgatgctgc
     4321 agaccgaagg cggccgcttc acgctgggtc gatcgacgat caccgacacc aagaactacg
     4381 gcaacatgac gctgagccag gcaatcgcga agtcgtccaa cgtggcgacc gcgcgcatgg
     4441 cgatgcagat gacgcccgag cagatgtggg acctgtttac ctcgctcggc ttcgggcagg
     4501 cgcccaagct cggtttcccc ggcgcggtgg cggggcgcgt gcggccggcg gcctcctggc
     4561 gtccgatcga gcaggtcacg atgagctacg gctacgggct gtcggcctcg ctgatccaga
     4621 tcgcgcaggc ctacatggtg ttcgcgcgcg acggcgacat gattccgctg acgctgcaga
     4681 agagctccga caagccgatc ggccggcgtg tgctgtcacc gcagacggcg cgcgaaatgc
     4741 ggctgatgct cgaaggcgcg gccagcccgg acggcaccgc gccacgcgca caggtgcccg
     4801 gctaccgtgt cggcggcaag accggcaccg cgcacaagct ggaaaacggg cgctacacca
     4861 acaagtacat cggctccttc gccggcatcg cgccgatctc ggacccgcgc ctgatcgtcg
     4921 cggtcatgat tgatgagcct tcagg
//
LOCUS       DATLGF010000465         6102 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_201740, whole genome shotgun sequence.
ACCESSION   DATLGF010000465 DATLGF010000000
VERSION     DATLGF010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6102
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201740"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2006)
                     /locus_tag="VM406_14430"
     CDS             complement(<1..2006)
                     /locus_tag="VM406_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HVL77208.1"
                     /translation="MRALLLMGVGGVIASVLLLAFALVVISPSLPSIEALTDYRPKIP
                     LRVYTADNVLIGEFGEERRDFVRIKDMPAVMKNAVVAVEDERFYEHSGVDYIGLMRAS
                     VANLGRSRSQGGSTITMQVARNFFLSREKTYTRKLQEVMLAYRIEQALSKDQILELYM
                     NQIYLGQRAYGFGSAARIYFGKPISQVTAAEAAMLAGLPKAPSTANPVVNPKRATQRQ
                     QYILRRMREQGYLTQAQYEQAIAERLTIRRDGGRFATQAGHAAELVRQHMVSLYKDDA
                     YTQGFSVYTTLLSTDQAAAHQALRRGVLEYERRHGYRGPEAFIDLPKSGEAREQAIGE
                     ALLRHPDSGELRAAMVVAANAKAVRAELITGEAIEISGEGLRFAAAALNEKAAAERRI
                     RPGAVIRVMQDARKRWAITQLPQVGAALVSLDARDGSVRAMVGGFDFSLNQFDRVTQA
                     WRQPGSAIKPFIYSAALEKGFYPATMINDAPLSEDVADGLTPASWQPQNDDGNYDGPI
                     TLRHALKRSKNLVSVRVLRTIGTPYALDYLERFGFDSARHPGNLTMTLGTGSVTPLQL
                     AGAYAVFANGGYQIKPYLISKVVDARGNVLSQADPVHAGDEAARVIDPRNAYLMDSML
                     RDAVNSGTGHQAAQRLGRHDLAGKTGTTNDAIDGWFAGYGGDVVA"
     gene            complement(2398..3522)
                     /locus_tag="VM406_14435"
     CDS             complement(2398..3522)
                     /locus_tag="VM406_14435"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HVL77209.1"
                     /translation="MALEEFKTSDALTLGVELELQLVSLSDFDLAEASPDMLDLLRRK
                     PFPGTVTPEITESMIEIATGIHTRHDELLSELQQIRNTLVHAGDTLNVGVCGGGTHPF
                     QRWSERKIFSKPRFKQVSALYGYLAKQFTVFGQHVHVGCESGDDAMFLLHSLNRYVPH
                     FIALSASSPFFQGRDTLFNSARLNSVFAFPLSGRAPFLLKWKDFEDVYFSRMEHTGIV
                     KSMKDFYWDIRPKPEYGTIELRVCDTPLTVERAAALACYLQALCRYLLDGGEVPPAED
                     DYLVYNYNRFQACRFGLDGTMVHPKTHETLSIREDVLTTLRKMEPFAEELGGVGALEH
                     LYRAAHMGSDASFLRQQYHERGSPEGMVDAAIRRFREGLA"
     gene            complement(3524..4711)
                     /locus_tag="VM406_14440"
     CDS             complement(3524..4711)
                     /locus_tag="VM406_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HVL77210.1"
                     /translation="MPEASPLATYVAWPFALAIAWVVGEMGYRMTGLPRISIYGLVGF
                     LLAHTQTGFLPAGDDVGVKLLADVAFGLILFELGYRINLNWLRTNPWIPLTGLVEAAA
                     TFVVVYAVARWFETPQMASLLLASLAMSTSPAGILRVVNEQTSSGQVTERVMHLSAIN
                     CVLAVFTFKLIVGVGVFQTSGSFTEATWSGLLVVLVSIALGGLFGVLMPALMRQLGNL
                     GRDATLGFAIGVILLVAITHAFRFSPVLATLAFGLVARHRRVILSPTQRNFGVMGDLL
                     TVLLFFYIATTLEWATVVEGMAVALVLIAARFVTKTSIVAAFSHVSGISWRKGVLTGV
                     ALTPISVFVILLLEQTRHLGITMVEGLIPLAAVALLLEVIGPIATQRALIWANETPDA
                     TES"
     gene            5034..5432
                     /locus_tag="VM406_14445"
     CDS             5034..5432
                     /locus_tag="VM406_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HVL77211.1"
                     /translation="MDLNEGQELQRCIDACLACYRTCQQTAMQHCLEVGGPHVEAQHM
                     RLMNACAEVCRASAHVMLAGIPQHAAVCAACAQICRACAESCEDIGDMDECVRACREC
                     AQSCEEMSGGELGLASPLSPAQANPGQLAM"
     gene            5441..5641
                     /locus_tag="VM406_14450"
     CDS             5441..5641
                     /locus_tag="VM406_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77212.1"
                     /translation="MQQTPEINPVSPPEIPATPGPGPEIAPQTPQPEIPEPPPDPGSP
                     NQPTGPEIQPEPTPPEYPGGPG"
     gene            5782..>6102
                     /locus_tag="VM406_14455"
     CDS             5782..>6102
                     /locus_tag="VM406_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HVL77213.1"
                     /translation="MRNRDVLFSDRRDAGRQLAARLLAMGLTRPVVLALPRGGIPVAF
                     EIARALGAPLGIVLVRKIGTPWSKEVAIGALAEVRRQHRVMSPSARQYAGAWLDEQIQ
                     VLQQE"
BASE COUNT         1102 a         2016 c         1976 g         1008 t
ORIGIN      
        1 gccacaacgt cgccgccgta tccggcgaac cagccgtcga tcgcgtcgtt ggtggtgccg
       61 gtcttgccgg cgagatcatg gcggcccagg cgctgcgccg cctggtggcc ggtgcccgag
      121 ttcaccgcat cgcgcagcat cgagtccatc agatacgcat tgcgcggatc gatcacacgc
      181 gccgcctcgt cgccggcatg aaccggatcc gcctgcgaca gcacattgcc gcgcgcgtcc
      241 accaccttgc tgatcaggta aggcttgatc tggtagccgc cattggcgaa caccgcatac
      301 gcgcctgcaa gctgcagcgg cgtcaccgag ccggtgccga gggtcatcgt cagattgccc
      361 ggatggcgcg cgctgtcgaa gccgaagcgc tccaggtaat cgagcgcgta cggggtgcct
      421 atcgtgcgca gcacgcgcac cgagaccagg ttcttcgaac gcttgagcgc atggcgcagc
      481 gtgatcggcc cgtcgtaatt gccgtcgtcg ttctgcggct gccagctcgc cggcgtcagg
      541 ccgtccgcca cgtcttccga aagcggcgca tcgttgatca tcgtggccgg gtagaagccc
      601 ttctccaggg cggccgagta aatgaagggt ttgatcgccg aaccgggctg gcgccaggcc
      661 tgcgtcacgc ggtcgaactg gttgagcgag aagtcgaagc cgcccaccat cgcgcgcacc
      721 gacccgtcgc gcgcatcgag cgacaccagc gccgcgccca cctgcggcag ctgggtaatg
      781 gcccagcgct tgcgcgcatc ctgcatcacg cggatcaccg cgcccggacg gatgcgccgt
      841 tccgctgcgg ccttttcgtt cagcgccgcc gcggcaaagc gcagcccttc gccgctgatc
      901 tcgatcgcct ctcccgtgat cagctcggcg cggaccgcct tcgcattggc ggccaccacc
      961 attgccgcgc gcagctcgcc gctgtcagga tggcgcagca acgcctcgcc gatcgcctgc
     1021 tcgcgcgcct cgccgctctt gggcaggtcg atgaacgctt ccgggccgcg ataaccgtgc
     1081 cgccgttcgt actcgagcac gccgcggcgc agcgcctgat gggccgctgc ctggtcggtg
     1141 ctcagcaagg tcgtgtagac ggaaaagccc tgagtgtatg cgtcgtcctt gtacagcgac
     1201 accatgtgct ggcgcaccag ctcggcggca tggccggcct gggtcgcgaa acgcccgccg
     1261 tcgcggcgta tcgtcagccg ctcggcaatc gcctgctcgt actgcgcctg ggtcagatag
     1321 ccctgctcgc gcatgcggcg caggatgtat tgctggcgct gcgtcgcgcg cttcggattg
     1381 accacggggt tggcggtcga cggcgctttc ggcaggcccg cgagcattgc ggcttcggcc
     1441 gccgtgacct ggctgatcgg tttgccgaag tagatgcgcg ccgcgctgcc gaagccgtag
     1501 gcgcgctggc cgagatagat ctggttcatg tacagctcca ggatctggtc cttgctcagc
     1561 gcctgctcga tgcggtacgc gagcatgacc tcctggagct tgcgggtgta ggtcttttcg
     1621 cgcgaaagga agaagttgcg cgccacctgc atcgtgatcg tcgatccgcc ctgcgagcga
     1681 ctgcggccga ggttggccac gctggcgcgc atcaggccga tgtagtccac gccgctatgc
     1741 tcgtagaagc gctcgtcctc gaccgccacg acggcattct tcatcactgc cggcatgtcc
     1801 ttgatgcgta cgaagtcgcg ccgctcctcg ccgaattcgc cgatcagcac attgtcggcc
     1861 gtatagacgc gcagcgggat cttgggacgg tagtcggtca gcgcctcgat cgacggcaag
     1921 ctgggagaga tgacgacgag tgcaaacgcc agcagcaata cagacgcgat cacgccgccc
     1981 acgcccatca gcagcaaagc gcgcagcaag ccgccaccgc gcgacgcctt gccgcccccg
     2041 cttcccttgc cattcttgct gcccttgccg gaagccggct tgcctcgtga acctgccaaa
     2101 accttcccct aaagaaactg ctgcaggcgt gactctagca gggtcgtgcg cggcgtgctc
     2161 gggctgttgc aaacgcttgc caggctggga acgcgcgccg gatcgggggc aggcagcgaa
     2221 ccgaaggcca cggggaacac ccaaaacaat gtcatcccga gcgcagcgag ggatctcatc
     2281 cgttcacaga accgggattc ctcgctgcgc tcggaatgac gcgttcccgc cgtgctcgcc
     2341 gtgtacgccc tgtacgccgt ggttcaaaca ctttgccgag gcaccggaac cagaccgtca
     2401 ggcgagccct tcgcggaagc gccggatggc cgcgtccacc atgccctcgg ggctgccgcg
     2461 ctcgtggtat tgctggcgca ggaagctcgc gtcgctgccc atgtgggcgg cgcggtaaag
     2521 atgctcgagc gccccgacgc cgccaagctc ttcggcaaac ggttccatct tgcgcagcgt
     2581 ggtcagcaca tcctcgcgga tcgacaaggt ctcgtgggtc ttcggatgca ccatcgtgcc
     2641 gtcgaggcca aaacggcagg cctggaaacg gttgtagttg tagaccaggt aatcatcttc
     2701 ggcgggcggc acttcgccgc cgtccagaag gtagcggcac aatgcctgca ggtagcaggc
     2761 cagcgcggcc gcgcgttcga ccgtcagcgg cgtgtcgcac acgcgcagct cgatcgtgcc
     2821 gtactcgggt ttcgggcgga tgtcccagta gaagtccttc atgctcttga cgatgcccgt
     2881 gtgctccatg cgcgagaagt acacgtcctc gaagtccttc cacttgagca ggaacggcgc
     2941 gcggccggac agcggaaatg caaataccga gttgagccgc gccgaattga acagcgtgtc
     3001 gcgcccctgg aagaatggcg acgatgccga caaggcaatg aaatgcggca cgtagcgatt
     3061 cagcgaatgc agcaggaaca tcgcgtcgtc gccggattcg cagccgacat gcacgtgctg
     3121 gccgaacacg gtgaactgct tggccagata gccgtacaac gcggagacct gcttgaagcg
     3181 cggcttggag aagatcttgc gctcggacca gcgctggaac ggatgggtgc cgcccccgca
     3241 gacgccgacg ttgagcgtgt cgcccgcatg caccagcgta ttgcggatct gctgcagttc
     3301 cgacagcagc tcgtcgtgcc gcgtgtggat gccggtggcg atctcgatca tgctttcggt
     3361 gatctccggc gtcaccgttc cgggaaacgg cttgcggcgc aacagatcga gcatgtcggg
     3421 gctggcttcg gccaggtcga aatcggacag gctgaccagc tgcaactcca gttcgacgcc
     3481 aagcgtcagc gcgtcggatg tcttgaattc ttccagggcc atgtcaactc tccgtcgcgt
     3541 cgggggtctc gttcgcccag atcagggcgc gctgcgtcgc gatcggcccg atcacttcga
     3601 gcagcagcgc aacggcagcc agcggaatga gcccctcgac cattgtgatg ccgaggtggc
     3661 gggtctgttc cagcagcagg atcacgaaga ccgaaatcgg cgtgagcgcc acgccggtca
     3721 gcacaccctt gcgccaggaa atcccgctga cgtgggaaaa cgcggccacg atggaagtct
     3781 tcgtgacgaa ccgggccgcg atcagcacca atgcaaccgc catgccttcg accacggtcg
     3841 cccactccag cgtggtcgcg atgtaaaaga acagcagcac ggtcagcaga tcccccatga
     3901 ccccgaaatt gcgctgggtc gggctcagga tcacgcggcg atgacgcgcc accaggccga
     3961 atgcaagcgt cgcgagcacc ggcgaaaaac ggaacgcatg cgtgatggcg accagcagga
     4021 tcacgccgat tgcaaagccg agtgtcgcat cgcgtccgag attgccgagc tggcgcatca
     4081 gcgccggcat cagcaccccg aacagcccgc ccagcgcgat tgaaaccagc acaaccagca
     4141 gtccgctcca cgtcgcttcg gtgaagcttc cggaagtctg gaacacgccg acgccgacga
     4201 tcagcttgaa tgtgaatacc gcgagcacgc agttgatggc cgacaggtgc atgacccgtt
     4261 ccgtcacctg gcccgagctg gtctgctcgt tcacgacgcg caggatgccg gccggtgagg
     4321 tcgacatcgc cagtgacgcc agcagcagcg atgccatctg gggcgtctcg aaccagcgtg
     4381 cgactgcgta gacgaccacg aaggtcgccg ccgcctccac cagcccggtc agtggaatcc
     4441 aagggttggt gcgcaaccag ttcaggttga tccggtagcc gagttcgaac aggatcagtc
     4501 cgaaggcgac gtcggcaaga agttttactc cgacatcatc tcccgccggc aggaaaccgg
     4561 tctgggtgtg cgccagcagg aagccgacaa ggccgtagat gctgatgcgg ggcagtcccg
     4621 tcatccgata tcccatttct cccacgaccc aggcgatcgc gagcgcgaaa ggccaggcga
     4681 cgtatgttgc tagcggagac gcttccggca tcggtttcct ttcattcgac agaaaaactg
     4741 cgcaattaat acccgacaat caggtcccgc ttcttgatat gtcccgtgta tttcacgctg
     4801 gacatgacgc ggaggtttgg cgttgcagga cggccagggc gggcgctatt gcgcgacatg
     4861 gggcggcatg gcgccgcggc tgggttgaaa ggcggcggca cgcggtctgg gcccgccccg
     4921 actgggtcgc ccccgaggtg ctccccggaa aggcgggcct attataccca agcgcttcga
     4981 ccgtcccggc acagatagtg ctgcgtgact cccgtctaat cgaggaggac ggaatggact
     5041 tgaacgaagg acaagagctg cagcgctgca tcgatgcctg cctcgcctgc taccgcacgt
     5101 gccagcagac tgccatgcaa cattgcctcg aagtcggagg gccgcacgtc gaagcgcagc
     5161 acatgcggct gatgaacgcc tgcgccgagg tctgccgcgc cagcgcgcac gtgatgctgg
     5221 ccggaattcc ccagcatgcc gccgtgtgcg cggcctgcgc gcaaatatgc agggcctgcg
     5281 cggaaagctg cgaggacatc ggcgacatgg acgaatgcgt ccgtgcctgc cgcgaatgcg
     5341 cgcaatcgtg tgaagagatg tcaggaggcg agctcggcct tgccagcccg ctctcgcccg
     5401 cacaggcgaa cccgggccag ctcgcgatgt aggaggacgg atgcagcaga ccccggaaat
     5461 caatccagtc agcccgccgg agattccggc cacgcccggc cccggcccgg aaatcgcacc
     5521 gcagaccccg cagcccgaga taccggaacc tcctccggac ccgggttcgc cgaaccagcc
     5581 gaccgggccg gagatccagc ctgaacccac tccgccggag tatccgggcg gacccggttg
     5641 atgcacggat cgccgtgcgg ccgcgacccg gcgcgtggtt cgtcaagcgt tgtaacttgt
     5701 accacgccgc ggtaaaaacg ggccgcccgg ccgcacctgt caagcgtatc gcgatggcag
     5761 gcatcttgct ggaagacggg catgcgcaat cgggatgttc tcttctcgga ccggcgcgac
     5821 gccggccggc agttggcggc gcgcctgctc gccatgggcc tgacacggcc ggtggtgctt
     5881 gcgctgccgc gcggcggcat accggtcgca ttcgagattg cacgcgccct gggcgcacca
     5941 ctgggaattg tgctggtgcg caagatcggc acgccatggt caaaggaggt ggcaatcgga
     6001 gcattggccg aggtgcgccg gcagcaccgg gtcatgtcgc cttccgcgcg ccagtatgcg
     6061 ggcgcgtggc tggacgaaca gatccaggta ctgcaacagg ag
//
LOCUS       DATLGF010000466         2534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_77897,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000466 DATLGF010000000
VERSION     DATLGF010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77897"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VM406_14460"
     CDS             complement(<1..420)
                     /locus_tag="VM406_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HVL77214.1"
                     /translation="MLPSIEQRLAAELAAKPAQVAAAIALLDEGATVPFIARYRKEAT
                     GGLDDTQLRLLEERLRYLRELEDRRAAIIASIEEQGKMTPELLDAIVHAEDKTRLEDL
                     YLPYKPKRRTKAQIAAEAGLTELADALLADPSLKPEDE"
     gene            complement(545..1309)
                     /locus_tag="VM406_14465"
     CDS             complement(545..1309)
                     /locus_tag="VM406_14465"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HVL77215.1"
                     /translation="MKTAPRQQPLLGILGGMGPLATVDFLAKLTRSLPASCDQEHLAW
                     LTLSQPGTPDRSRAIQNGDNGPHPYLTQGAAWLSGQGVSLIAIPCSTSHYWYEAMQRA
                     SSAPILHIADATIEELLLGGDASGPVAVLATRGTVRHRIYSGRLHAAGCEVAALDENA
                     QRAVDGIIRDVKAGQLESARREMHVLKARLKLRGVRAVVLGCTELPIAHDDTDPSLPA
                     IDVTLALARAALRRLGYPTVALPLANSLASAALPGD"
     gene            complement(1306..>2534)
                     /locus_tag="VM406_14470"
     CDS             complement(1306..>2534)
                     /locus_tag="VM406_14470"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HVL77216.1"
                     /translation="LSLIGDVYIDLLKMVILPFLVSAVIVSINRLATDPSASRYLGQI
                     AFFLIAGMAAAGIAGALYMTMVQPGARLDADALNAFGRLVQSDLSGVETSIQLLAPAA
                     AAQESAAASLIMRMIPDNVFAALSLGDTLKTLFFALAFGFALAATPRDKTISLIDCCE
                     AVFRACQRLTTWLLYGLPVASFALAADQIAKTGVEPFRVMIHFIVAFAIVTALLLALC
                     VFILYRRSGRPIGEVLRSQQSPFFIAVATRNSAACMPAMMASLIDRLRFDKGITELLV
                     PLGTALFRVGPVLYYCMATIFIAQLYGRDLSLADLGIVLAASLIAGFSSTGMNGIVTI
                     SQTAIVCSVLGLPFEAAFVLFVAVEPVCDTLRTVLLVFGINALVALISPLDESDEDDP
                     VPQPHPAFEHARRRAA"
BASE COUNT          447 a          813 c          883 g          391 t
ORIGIN      
        1 ctcgtcctcc ggcttcagcg aaggatcggc cagcagcgca tcggccagtt cggtgaggcc
       61 cgcctcggcg gcgatctgcg ccttggtgcg gcgcttgggc ttgtacggca ggtacaggtc
      121 ctccagccgc gtcttgtcct cggcgtggac gatcgcatca agaagctccg gggtcatctt
      181 gccctgctcc tcgatcgagg cgatgatcgc cgcgcgccgg tcttccagtt cgcgcaggta
      241 gcgcaggcgc tcttccagca ggcgcagctg ggtatcgtcg aggccgccgg tggcttcctt
      301 gcggtagcgg gcgataaacg ggacggtcgc gccctcgtcc agcagggcga tggcagcggc
      361 cacctgggcc ggcttggcgg cgagttcggc ggcgaggcgt tgttcaatcg aaggcagcat
      421 gtcggctcac atcaggaaaa caagtcggac atgatatgca atcttcgcga ttgcgccagt
      481 cttgacacgg gctgaaacag cgggcgttcg catcggactg cggcaaagcg cgggcggaaa
      541 gagcttagtc gcctggcaat gcggcggatg ccaggctgtt cgcgagcggc aaggccacgg
      601 tcgggtaacc gagccgccgc agcgccgcac gcgccagcgc cagcgtgacg tcgatggccg
      661 ggagcgaagg atcggtgtcg tcgtgcgcga tcggcagttc agtgcagccg agcacgacgg
      721 cgcgcacccc gcgcagcttc agccgcgcct tcagcacatg catttcacgg cgcgcggact
      781 ccaattgccc agccttgacg tcgcggatga tgccgtcgac cgcgcgctgc gcgttctcgt
      841 cgagcgcggc cacttcgcat cccgcggcgt gcaggcggcc gctgtagatc ctgtgacgga
      901 cggtcccgcg cgtggccagc acggcaaccg gcccacttgc gtcgccgccc agcaggagtt
      961 cttctatcgt cgcgtcggcg atgtgcagga tcggcgcgga ggatgcgcgc tgcatcgctt
     1021 cgtaccagta gtggctggtg ctgcacggaa tggcgatcag gctcacgccc tggccggaca
     1081 gccaggccgc accctgcgtc aggtatggat ggggtccgtt gtcgccgttt tggatcgcgc
     1141 ggctgcggtc cggggtgccg ggctggctca gcgtgagcca cgccaggtgc tcctggtcgc
     1201 aggacgccgg cagcgagcgc gtcagcttgg caaggaagtc gaccgtggcc agcggcccca
     1261 tgccgccgag tatgccgagc aaaggctgct ggcgcggcgc ggtcttcatg cggcccgcct
     1321 gcgcgcgtgc tcgaacgccg ggtgcggttg cggtaccggg tcgtcctcgt cgctctcgtc
     1381 gagcggcgag atcagcgcca ccagcgcatt gatgccgaaa accagcagca ccgtgcgcag
     1441 cgtgtcgcat accggctcga ccgcgacaaa cagcacgaag gccgcctcga aaggcagacc
     1501 gagcaccgag cagacaatgg cggtctgcga aatcgtcacg atgccgttca tgccggtcga
     1561 agagaagccg gcgatcagcg aggcggccag aacgatgccg aggtcggcga gcgacaggtc
     1621 gcggccgtag agctgtgcga tgaagatcgt cgccatgcag tagtagagca ccgggccgac
     1681 gcgaaacagc gccgtgccca gcggcaccag cagttccgtg atgcccttgt cgaagcgcag
     1741 acggtcgatc agcgaggcca tcatcgccgg catgcaggcg gcgctgttgc gcgtcgccac
     1801 cgcgatgaag aacggactct gctgcgagcg caggacctcg ccgatcggcc ggccggacct
     1861 gcgatacagg atgaacacgc acagcgcgag caacagcgcg gtgacgattg cgaacgcgac
     1921 gatgaaatgg atcatcaccc ggaacggttc gacgccggtc ttggcgatct ggtcggcggc
     1981 cagcgcgaac gatgcaaccg gcaggccgta gagcagccag gtggtcaggc gctggcaggc
     2041 gcggaacact gcttcgcagc aatcaatcag gctgatcgtc ttgtcgcgcg gcgtggcggc
     2101 aagtgcaaag ccgaacgcca gcgcaaagaa cagggtcttg agcgtatcgc ccagcgacag
     2161 tgcggcaaac acattgtccg gaatcatccg catgatgagg ctcgcggccg cgctctcctg
     2221 cgccgccgcc gcgggcgcaa gcagctggat gctggtttcc acgcctgaca gatcggactg
     2281 cacgagccgg ccgaacgcgt tgagcgcgtc ggcatccaga cgtgcgcccg gctgcaccat
     2341 ggtcatgtag agcgccccgg cgatgcccgc cgccgccata ccggcgatca ggaagaacgc
     2401 gatctggccc aggtatctgg acgccgatgg atcggtcgcg agccggttga tgctgacgat
     2461 caccgcggat accaggaatg gcaggatcac catcttcagc aggtcgatgt agacatcgcc
     2521 gatcaggctg agac
//
LOCUS       DATLGF010000467         4270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_13385,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000467 DATLGF010000000
VERSION     DATLGF010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13385"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..1158)
                     /locus_tag="VM406_14475"
     CDS             complement(241..1158)
                     /locus_tag="VM406_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL77217.1"
                     /translation="MKAHISTRLLYAFLALEELRHFTRAAERCHTSQSAFSVMIQKLE
                     AAVGARLFERDTRKVVLTPEGELFAEVARSLTGEIESAFDDMADYVAKRRGRVAVAAL
                     PSLAARGMPAVLAEYRRRHPGVAVSLHDALSDQCLALLRQGRVDFVLTAPGPNVDEFE
                     SHILCSDPFYLVCRRDHPLAKKRSLKAADLADCELVHLVGSTSVRQHVDALLREVPTR
                     HSGFEVEHLATLAGLVEQGLGVTIVPRLTLFQFEQLDLATVPIETRAHVRPILVVKRR
                     DRSLSIAAQAMLDLVQAALGQASGGRQRA"
     gene            1279..2616
                     /locus_tag="VM406_14480"
     CDS             1279..2616
                     /locus_tag="VM406_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HVL77218.1"
                     /translation="MRRIRIGAGAGYSGDRIEPAVELAERGDLDYLIFECLAERTIAL
                     AQQARMKDPQAGYDPLLEARMRAVLPLCRANGIRILTNMGAANPQGAAECVRRIAREL
                     NLTGLKIAAVSGDDVLERVRDGGLRCDGPVAVEALGERLLSANAYIGAASLVDALAAG
                     ADVIVCGRVSDPALVLAPLIHEFGWAMDDWPRLGQGTLAGHLLECAGQITGGYFADPG
                     FKDVPDLARLGFPIGEIGEDGSIVITKVAGSGGKVTPATCKEQLLYEIHDPAAYLTPD
                     VIADFSKVEIRELGPDRVLVSGGTGRARPPELKVSVGYIDSYIGEGQMSYAGPGAVAR
                     ARLALQIVQERLRITQVRTTELRTEMIGIDAMHGAALSARHPEPYEVRIRVAGRADTL
                     NEAIRIGNEVESLYTCGPAGGGGATKSAREVVAVMSTHLPRDQVHTAVHMIEA"
     gene            2618..2926
                     /locus_tag="VM406_14485"
     CDS             2618..2926
                     /locus_tag="VM406_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77219.1"
                     /translation="MQLRDIAHTRAGDKGNISNISVIAYDTKDYALLERFVTPERVKD
                     HLSGIVSGEVVRHALPHLGALNLVLHGSLAGGVTRSLALDAHGKCLSFALLSLDIPDD
                     "
     gene            3008..3958
                     /locus_tag="VM406_14490"
     CDS             3008..3958
                     /locus_tag="VM406_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVL77220.1"
                     /translation="MSSLLLSAACLVGAGSAHAQYPDKPIRFVVPFAAGTATDQFARA
                     LGQAISQETKQPVVVDNKPGASGFLGATDVARAAPDGYTVLIATNTTHAANEHLFKKL
                     PYDPIKDFAPISGLGRGGQVMVVNVNAPIKSVSEFIAAAKKNPGRMSFASGSSSSRIA
                     GELFQQMAGVQLLHVPYKSNTFAVTDVLGGQVDMMITDTATGLPHVASGKLRALGMSA
                     SKRSPLAPDVPTIAEAGVPGYEMTFWFAAYAPANTPPAVVKRLNELFANAVKGPATQN
                     FFNTTGVEAFITSPEELGRFQVSESKKWEEIIRKAGIEKE"
     gene            complement(4035..>4270)
                     /locus_tag="VM406_14495"
     CDS             complement(4035..>4270)
                     /locus_tag="VM406_14495"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HVL77221.1"
                     /translation="MDLSTSFNRYAVRWEPNRQIFYFNGQEILRVNVTMSDPMYLMLD
                     LWFGSASGTPDHMTPQGKTNSFEINYMRAWQFR"
BASE COUNT          791 a         1409 c         1379 g          691 t
ORIGIN      
        1 gggaaatcca gcgggggaag atcccagtcc cggttcggtt cgcgccgtgc gcagaaaatc
       61 acgttgagtt cgattccggc ggtgcgcgca atgcgctcga acaccggaat ccggtacggc
      121 ggcggttcgt tggtgacgag ggcgattttt ttcatgggcc tatggtaagg ccctcgttgc
      181 cgcaggggat gacttacatc atgaagcgcc ccggaatacg tgtgcgccgc atgccgccga
      241 tcatgcgcgc tgcctgcccc cgctcgcctg ccccagcgcg gcttgcacga gatcaagcat
      301 cgcctgcgcg gcgatcgaca agctgcggtc gcgccgcttc accaccagga tcggtctgac
      361 gtgggcgcgc gtttcgatcg gcacggtcgc caggtcgagc tgctcgaact ggaacagcgt
      421 cagccgcggc acgatcgtca cccccagccc ctgctcgacc agtcctgcca gcgtggccag
      481 gtgctcgacc tcgaagccgg aatgccgggt cggcacctcg cgcagcaagg cgtcgacgtg
      541 ctggcgcacg ctggtggagc cgaccaggtg caccagctcg cagtcggcca ggtcggccgc
      601 cttcagcgaa cgcttcttcg caagcggatg gtcgcgccgg cagaccaggt agaacgggtc
      661 gctgcacagg atgtgcgact cgaactcgtc gacgttcggc cctggtgcgg tcaggacgaa
      721 atccaccctt ccctgcctca gcaatgccag gcactggtcc gacagcgcat cgtgcagcga
      781 cacagccaca cccggatggc ggcgccgata ctcggccagc acggccggca tcccgcgcgc
      841 ggccagcgaa ggcagcgccg ccacggccac gcggccgcgc cgcttcgcga cgtagtcggc
      901 catgtcgtcg aacgccgact cgatctcgcc tgtcagcgat cgggcgacct cggcaaacag
      961 ttctccctca ggggtcagca ccaccttgcg cgtgtcgcgc tcgaacaggc gcgcaccgac
     1021 cgcagcctcc agcttctgga tcatgacgct gaacgccgat tgcgaggtgt ggcaccgctc
     1081 ggccgcgcgg gtgaaatgcc gcagctcctc cagcgcgagg aacgcataca gcagacgggt
     1141 ggaaatatgg gccttcatcg ttaatcgtaa aattcgatca atcaatacaa aaataccatt
     1201 ttacagatca aaagcgtcgt cacagaatgc tccgaaacat gcgggtcagg ccactgcgcc
     1261 ccgacccggg agaccaggat gagacgaatc agaatcggag caggagcagg ctattcgggc
     1321 gaccgtatcg agccggccgt cgagcttgcc gaacggggcg atctcgacta cctgatcttc
     1381 gagtgcctgg ccgagcgcac catcgcgctg gcccagcagg cgcgcatgaa ggacccgcag
     1441 gccggctacg atccgctgct cgaggcgcgc atgcgcgccg tgcttccgct gtgccgcgcc
     1501 aacggcatcc gcatcctgac caacatgggc gccgccaatc cgcaaggcgc ggcagaatgc
     1561 gtgcgccgca tcgcccgcga gctgaacctg acgggcctga agatcgctgc ggtaagcggc
     1621 gacgatgtgc tggagcgcgt gcgcgacggc gggctgcgct gcgacggacc ggtggcggtc
     1681 gaggcgctcg gcgagcggct cttgtcggcc aatgcgtaca ttggagcggc gtcgctggtc
     1741 gacgcgcttg ccgccggggc ggacgtgatc gtctgcgggc gcgtgtcgga cccggcgctg
     1801 gtgctggcgc cgctgatcca tgagttcggt tgggcaatgg acgactggcc gcgcctcggt
     1861 caaggcacgc tcgccggcca tctgctcgaa tgcgcgggcc agatcaccgg cggctacttc
     1921 gccgatcccg gcttcaagga tgtgccggac ctggcccgcc tcggatttcc gatcggcgag
     1981 atcggcgagg acggctcgat cgtgatcacc aaggtcgccg gctccggcgg caaggtaacc
     2041 cccgccacct gcaaggaaca gctgctgtac gagatccacg acccggccgc ctacctgacg
     2101 ccggacgtga tcgcggattt ttcgaaggtc gagatccgcg aactcggacc ggaccgcgtg
     2161 ctggtcagcg gcggcaccgg ccgcgcgcgt ccgcccgagc tcaaggtctc ggtcggctac
     2221 atcgacagct atatcggcga ggggcagatg tcgtacgcgg ggccgggcgc ggttgcgcgc
     2281 gcgcggctgg cgctgcagat cgtgcaggag aggctacgga tcacgcaggt gcgcaccacc
     2341 gaactgcgca ctgagatgat cgggatcgat gcgatgcacg gggcggccct gtccgcccgc
     2401 caccccgagc cctacgaggt gcgcatccgc gtggccgggc gcgccgacac attgaacgag
     2461 gcaatccgca tcggcaacga ggtggagagc ctgtacactt gcggcccggc cggcggcggc
     2521 ggcgcgacga aatccgcgcg cgaggtcgtc gcggtgatgt cgacccatct gccgcgcgac
     2581 caggttcaca cggcggtaca catgatcgag gcatgacatg cagctacgcg acattgcaca
     2641 cacccgcgcg ggcgacaagg gcaacatctc gaacatttcc gtgattgcct acgatacgaa
     2701 ggactacgcg ctgctcgaac gcttcgtcac gcccgagcgc gtgaaggacc acctgtcagg
     2761 catcgtcagc ggcgaggtgg tgcgtcatgc gcttccgcac cttggcgcgc tgaaccttgt
     2821 gctgcacggt tcgctggcgg gcggggtcac ccgttcgctc gcactcgatg cgcatggcaa
     2881 gtgcctgagt tttgcgctgc tcagtctgga tatccccgac gactgagcac atcatcaacc
     2941 gccgtcaaaa ggcggatctc aacaacccac cgtccaaatc aaggagacca acgtgaaatt
     3001 caagaaactg tcctctcttc tgctgtcggc cgcatgcctg gtcggcgccg gctcggccca
     3061 tgcccagtat ccggacaagc cgatccgctt cgtcgtgccg tttgccgccg gcaccgccac
     3121 cgaccagttt gcgcgcgcgc tcgggcaggc gatttcgcag gaaacgaagc aaccggtcgt
     3181 agtggacaac aagcccggag cgagcggctt tctcggcgca accgacgtcg cgcgcgcggc
     3241 cccggacggc tatacggtac tgatcgcaac taatacaacg cacgcggcca acgaacacct
     3301 gttcaagaag ctgccttacg acccgatcaa ggattttgcg ccgatcagcg gactgggccg
     3361 cggcggccag gtcatggtgg tcaacgtgaa tgcccccatc aagtcggtat cggagttcat
     3421 cgcggcagcg aagaagaacc ccggccgcat gagcttcgct agcggcagct catccagccg
     3481 catcgccggc gaactgttcc agcagatggc cggcgtgcaa ctgctgcacg tcccgtacaa
     3541 gagcaatacc ttcgccgtga ccgacgtgct gggcggccag gtcgacatga tgatcaccga
     3601 taccgcaacc ggcctgccgc acgtcgcaag cggcaagctg cgcgcgctcg gcatgtcggc
     3661 ttcgaagcgt tctccgctcg cgcccgacgt gccgaccatc gccgaagccg gtgtgccggg
     3721 ttacgagatg acgttctggt tcgccgccta cgccccggcc aacacgccgc cggccgtggt
     3781 caagcgcttg aacgaactgt tcgccaatgc cgtcaagggg ccggccacgc agaacttctt
     3841 caacaccacc ggagtggaag ccttcatcac ctcgccggaa gaactcggac gcttccaggt
     3901 cagcgaatcg aagaagtggg aagagatcat ccgcaaggcg gggatcgaga aggaataagc
     3961 gtacggttca gtacgcaaaa agggtggcgg atcgttccgc cacccttttt tcattggtgc
     4021 accgtgtcag ccgatcaacg gaactgccag gcgcgcatgt agttgatttc gaacgagttg
     4081 gtcttgccct gcggggtcat gtgatcgggc gtgccgctgg cgctgccgaa ccacaggtcg
     4141 agcatcaggt acatcggatc gctcatggtc acgttcacgc gcaggatctc ctggccgttg
     4201 aagtagaaga tctggcgatt cggctcccaa cgcaccgcat agcggttgaa gctggtcgac
     4261 aggtccatgc
//
LOCUS       DATLGF010000468         2844 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_13428,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000468 DATLGF010000000
VERSION     DATLGF010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13428"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VM406_14500"
     CDS             complement(<1..368)
                     /locus_tag="VM406_14500"
                     /inference="COORDINATES: protein motif:HMM:NF045262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77222.1"
                     /translation="MRDLFILPSSCPYTEREFAARYEAGLQQTLPYLASGAVLLVLLF
                     GLWDYWIDPAGARLSVPVRLACAALAALAIWPDLLRWSASRRASFIHWTCSAAVLFAT
                     FTLDGALTFGLPGLVVGPFLV"
     gene            complement(507..2822)
                     /locus_tag="VM406_14505"
     CDS             complement(507..2822)
                     /locus_tag="VM406_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HVL77223.1"
                     /translation="MPDKTIPIRPYTAPAGGLGSLRGAANALATNGVLPAATVFPRLN
                     QPQGTDCPGCAYPDSPHSKSVDFCEQGAWAVAHEATPKRADADFFARHTLAQLRALDH
                     WELEAAGRLTTPLRYDAASDRYLPIGWDEAFALAATSLNTLTSPNDAVFYASGRSSNE
                     AAFPYQLFARAFGTNNLPDSSNYCHESSGDALKESIGVGKSTVTRADFEQADAIFIFG
                     QNPASNHPRMLADLREAKRRGCRIVVFNALIERGLQAFTDPQSPLEMLGGGSTPIADH
                     YFQVRIGGDLAALTGIIRALFEADAAARADGRASLLDHAFLAGHTTGFDALRDAILQQ
                     PWERIEEDSGLTRAQLETAAQVYAQSQAVIATWCMGLTQHEYAIPTIQMLVNLMLLRG
                     NVGKPGAGVMPVRGHSNVQGDRTVGITNRPKSAWLDALAREFGFEPPREHGLDAAKTI
                     RGLLDGTVRAFVALGGNFAVAGPDSPRVLASLSRCALTVHIATKLNRTHLYPGKTGLL
                     LPCLGRTEQDVQPTGPQFVSVEDTASMVHASHGRNRPASEMLRSEPWIVAELAHATLG
                     SARIDWRAMAADYDRTRAAIERVTRDVVQGFESYNERIRAQRGFHLPNAAAERRWETE
                     SGKARLIAHPLPQDTILRRARARHGDAVLCLSTIRAHRQYNTTVYRDRNGEVDRYRGV
                     HGTRHVLFISDADLKRFGLRDGDRVNLRAASLDGVERRVEGLQLVRYAVHPGDVFGYF
                     PELTPLLSPDFTARGSNTPTFKEIPVLIETA"
BASE COUNT          460 a          909 c         1029 g          446 t
ORIGIN      
        1 accaggaacg gaccgacgac gagaccgggg aggccgaacg tcaatgcgcc gtccagcgtg
       61 aacgtggcaa acaggacggc cgccgaacag gtccaatgga tgaagctcgc gcgccgggac
      121 gcgctccatc gcagcaggtc cggccatatc gcgagcgcag caagcgcggc gcaggcgagc
      181 cgcaccggca cggagagccg ggcgccggcc gggtcgatcc agtagtccca gagaccgaac
      241 agcagcacga gcagaaccgc tccggaagcg agatagggca gcgtctgctg cagccccgcc
      301 tcgtagcggg cggcgaattc gcgttccgta taggggcagg aggacggcag gatgaagaga
      361 tcgcgcaaga cgggctccag gagcggcgtg tcggttaggc aactgatgcg attctatcga
      421 cgtcgccacg ccggatttcc ggggccagat caaacttttc agattctccg ccgcggcgcg
      481 gcgaacgcgc cacagcgctg cctgcctcaa gccgtctcga tcagtaccgg gatctccttg
      541 aaggtcggcg tgttcgatcc ccgcgcggtg aaatccggag acagcagcgg cgtcagctcc
      601 gggaagtagc cgaacacgtc gcccggatgc accgcatagc gcaccagctg caacccctcg
      661 acgcgccgct ccacgccgtc cagcgaggcc gcccgcaggt tcacccggtc gccatcccgc
      721 aggccgaaac gcttcaggtc agcgtcgctg atgaacagca catggcgggt gccgtgcacg
      781 ccgcgatagc ggtcgacctc gccgttgcga tcgcgataga cggtggtgtt gtactggcga
      841 tgcgcgcgta tcgtcgacag gcacagcacc gcgtcgccat ggcgggcacg ggcgcggcgc
      901 aggatcgtgt cctgcggcag cggatgcgcg atcaggcgcg ccttgccgct ctcggtctcc
      961 cagcgccgtt cggccgccgc gttcgggaga tgaaagccac gctgcgcgcg gattctctcg
     1021 ttgtagcttt cgaagccctg cacgacatcg cgggtgacgc gctcgatcgc ggcgcgggtg
     1081 cggtcatagt cggcggccat cgcgcgccag tcgatgcgcg cgctgcccag cgtggcgtgg
     1141 gcgagctcgg cgacgatcca cggttccgaa cgcagcatct cgcttgccgg gcgattgcgg
     1201 ccatgcgagg cgtgcaccat gctggccgtg tcttcgacgc tgacgaactg cggcccggtc
     1261 ggctgcacgt cttgttcggt gcgtccgagg cagggcagca gcagccccgt cttacccgga
     1321 tagagatgcg tacggttcag cttggtggcg atatgcacgg tcagcgcgca gcgcgacagg
     1381 cttgcgagaa cgcgcgggct gtccgggccg gccaccgcga agttgccacc caaggcgaca
     1441 aaggcgcgca ccgtgccgtc gagcaggccg cgtatcgttt tcgccgcgtc cagcccgtgc
     1501 tcgcgcggcg gctcgaagcc gaactcgcgc gcgagcgcat ccagccaggc tgacttgggc
     1561 cggttcgtga tgccgaccgt gcggtcgcct tgcacgttcg agtggccgcg caccggcatc
     1621 acgccggcgc cgggcttgcc gacgttgccg cgcaagagca tcaggttgac cagcatctgg
     1681 atcgtcggga tcgcatactc gtgctgggtc aggcccatgc accaggtggc gatcactgcc
     1741 tgcgattgcg catacacctg cgcggccgtc tccagctgcg cgcgcgtgag gccgctgtct
     1801 tcctcgatgc gctcccaggg ctgctgcagg atggcgtcgc gcaaggcgtc gaagccggtc
     1861 gtgtgcccgg cgaggaacgc atgatcgagc agggaagcac ggccgtcggc gcgcgcggcg
     1921 gcatcggcct cgaacaatgc gcggatgatg ccggtcagtg cggcgagatc gccgcctatg
     1981 cgtacctgga aatagtggtc ggcgatcggg gtcgagccac cgcccagcat ctccagcggc
     2041 gactgcggat cggtgaatgc ctgcaggccg cgctcgatca gtgcgttgaa gacgacgatg
     2101 cggcagccgc gccgcttggc ttcgcgcagg tcggcgagca tgcgtggatg gttcgatgcc
     2161 gggttttggc cgaagatgaa gattgcgtcg gcctgctcga agtcggcgcg cgtgacggtc
     2221 gacttgccga cgccgatcga ttccttcagc gcgtcgcccg acgattcatg gcaatagttg
     2281 gatgagtccg gcaggttgtt ggtgccgaag gcgcgcgcga acagttgata tggaaaggcc
     2341 gcctcgttgc tgctgcggcc ggatgcatag aacaccgcat cgttcgggga ggtgagcgtg
     2401 ttcagcgacg tcgcagcgag agcgaaggcc tcgtcccagc cgatgggcag gtagcggtcg
     2461 ctggcagcgt cgtagcgcag cggcgtggtc aggcggcccg cggcttcgag ttcccaatgg
     2521 tccaatgcac gcagttgcgc aagcgtatgg cgcgcgaaga aatccgcgtc ggcgcgcttc
     2581 ggcgtggctt catgggcgac cgcccacgcg ccctgttcgc agaagtccac gctcttggaa
     2641 tgcggcgaat cgggataggc acagccgggg cagtcggtgc cttgcggctg gttcaggcgc
     2701 gggaagacgg ttgccgccgg cagcaccccg ttggtggcga gggcgttggc cgcgccgcgc
     2761 agcgagccga ggccgccggc gggcgcggtg tacgggcgga tcgggatggt cttgtctggc
     2821 atggcgggtc tcggttgggc gagg
//
LOCUS       DATLGF010000469        11272 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_122725, whole genome shotgun sequence.
ACCESSION   DATLGF010000469 DATLGF010000000
VERSION     DATLGF010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 11272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11272
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122725"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VM406_14510"
     CDS             complement(<1..773)
                     /locus_tag="VM406_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HVL77224.1"
                     /translation="MKQTLQLRMSQHLALTPQLQQSIRLLQLSTLELHQELEQILSDN
                     PLLERMDDPLDHSVRLLADGAISPGPAVNGSAEAPQLASDGGAEEPNGAADGGDVPDA
                     PAGAEGDWSFDDVARTSKAPDDDDARPQLEAPGITLREHLLDQMRLTVRESRDRALVE
                     LIIDALNDNGYLDEPLEEIHARLPEELGIDLDELRVALTLVQSFEPAGIGARNASECL
                     ALQIRRLPKIPLITRRMALNIVENHLTLFAQRDFNKLRKL"
     gene            complement(785..1531)
                     /gene="lptB"
                     /locus_tag="VM406_14515"
     CDS             complement(785..1531)
                     /gene="lptB"
                     /locus_tag="VM406_14515"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HVL77225.1"
                     /translation="MTTSTLVVRGLQKSYGSRQVVHDVSLQVSSGEVVGLLGPNGAGK
                     TTSFYMIVGLVPLDAGEIDLDDHAISRLPIHRRAMLGLSYLPQEASVFRKLTVEENIR
                     AVLELQLDKNGKRLGRDEIDRRLNALLADLQIERLRENQALSLSGGERRRVEIARALA
                     TNPRFVLLDEPFAGVDPIAVIEIQRIVRFLKERGIGVLITDHNVRETLGICDRAYIIN
                     QGAVLASGRPDDIIRNESVRRVYLGEHFRM"
     gene            complement(1528..2076)
                     /gene="lptA"
                     /locus_tag="VM406_14520"
     CDS             complement(1528..2076)
                     /gene="lptA"
                     /locus_tag="VM406_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HVL77226.1"
                     /translation="MKRTIHMLLVALAMATGTAHAARADSEQPTNIEADRMVYDDIKQ
                     INTFTGNVVLTRGSLVMRAHRLVVSQDPQGYQYATLEAAPGGLATFRQKRDSGPDDWV
                     EGEAERIEYNGKAETVHLIRRANMRRLEGTRPIDEVQGASITYDSQKEFYTVNNTAGG
                     ESKPGAGRIRATIQPRTEARSQ"
     gene            complement(2114..2698)
                     /gene="lptC"
                     /locus_tag="VM406_14525"
     CDS             complement(2114..2698)
                     /gene="lptC"
                     /locus_tag="VM406_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HVL77227.1"
                     /translation="MRHQSRFRLVAILAPVIALALASFWVVEVMRRGADDFIDRGQRA
                     EPDFYVEQFNFVRMSRTGDAQYHVAGTRLTHNPLDDSYDVQEPVVRALRGGEEPMTVR
                     AERAWINSDTSEIHLHENVHMDRPASGSQERLQLHSQHMIVFPDDDVMQTDKPVKITR
                     GKSTLFGTGMYANNATREFKLHSRVHGTYQPPPR"
     gene            complement(2701..3228)
                     /locus_tag="VM406_14530"
     CDS             complement(2701..3228)
                     /locus_tag="VM406_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HVL77228.1"
                     /translation="MLEQTVSRAADVRLMIFDVDGVLTDGSLHYGKDGEHIKRFNVLD
                     GHGMKLLQRAGVTTAIISARESEIVTRRAADLGVTHVFQGAHDKREAFARLLAQTGMD
                     ATHCGYIGDDVIDLPILLQVAFAASVPNGHPEVRGRVHYVTTAAGGHGAVREVCDFIL
                     KARGSYEAALAPYLG"
     gene            complement(3230..4249)
                     /locus_tag="VM406_14535"
     CDS             complement(3230..4249)
                     /locus_tag="VM406_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HVL77229.1"
                     /translation="MSKSEAKTSPASLPDTASRAMELARETLQIEADAILHLKARLDD
                     DNRFAHAVALLLQCSGRVVVSGIGKSGHIARKIAATLASTGTPALFVHAAEALHGDLG
                     MITENDALIAISNSGEAAEFLAIIPILRRMGAKLIAITGNEDSRLARLADIHLNARVT
                     KEACPLNLAPTASTTAALALGDALAVALLDARGFREEDFARSHPGGALGRRLLTHVRD
                     VMRSGDAVPSVTPELALPHALLEVTRKGMAMTAIVDGANRVLGVFTDGDLRRLIENVQ
                     DLNAVTIGDVMHRNPHTVRPEQLAVDAVELMERYRINQLLVTAEDGTLVGALHIHDLT
                     RAKVI"
     gene            4430..5335
                     /locus_tag="VM406_14540"
     CDS             4430..5335
                     /locus_tag="VM406_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HVL77230.1"
                     /translation="MALFERILGIILPVFLIIGLGYAYARWRPDAVRSDMAAVNRISI
                     GVLCPLLVFTALASKDFDVVHNATLILAGVGIALGSGLLAWPLARIFGYDVRTFVPPM
                     MYNNCGNMGLPLAVLAFGASGLPAAVAMFMACNLIYFSVGIRIIESGRGVHTPFWQFL
                     ANPMMMAMLAGLAFAFLRVPLPEELMRAMRMLGEASIPIMLFALGVRMLDVSLNSWRV
                     GLAGAVVCPAAGLAVAWLLDGVLTLNEAQRGQMFLFAALPPAVFCFMIAEQYRQEPDK
                     VASIVLLGNLAALGFVPLGLWMGMR"
     gene            complement(5342..5614)
                     /locus_tag="VM406_14545"
     CDS             complement(5342..5614)
                     /locus_tag="VM406_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HVL77231.1"
                     /translation="MARHLKITGRVQGVGYRASFEAQARALRLRGWVRNRRDGSVEAL
                     VDGDEDAVAQMVGWAHEGPPAARVDAVVLSEVDDPPGEGFAVLPTA"
     gene            complement(5623..8472)
                     /gene="uvrA"
                     /locus_tag="VM406_14550"
     CDS             complement(5623..8472)
                     /gene="uvrA"
                     /locus_tag="VM406_14550"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HVL77232.1"
                     /translation="MEEIRIRGARTHNLKNINLELPRNQLIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLQLMEKPDVDLIEGLSPAISIEQKATSHNPRSTVGTVT
                     EIHDYLRLLYARVGTPYCPQHPEHPLAAQSVSQMVDAVLALPEDTKLMILAPVVANRK
                     GEHADLFEHMQAQGFVRFRIQSGTQAAKIYEVDDLPKLKKTEKHTIDVVIDRLKVKAD
                     VKQRLAESFETALRLADGRAIALEMDGGREHLYSNKFACAICGYSLQELEPRLFSFNN
                     PMGACPECDGLGHIEFFDPKRIVAFPNLSLASGAIKGWDRRNQFYFQMLSSLAQHYGF
                     DLDEPFDSLPDKAQQAVLYGSGKEAVPFTYINERGRAVVREHTFEGVVNNLQRRYRET
                     DSMAVREELAKFINQKQCPECHGARLRVEARHVKIGSGRQQRAIYEIAAKPLRDALAF
                     FESLKLTGAKKEIADRIIKEIVSRLKFLNNVGLDYLSLERSADTLSGGEAQRIRLASQ
                     IGSGLTGVMYVLDEPSIGLHQRDNDRLIATLKHLRDIGNSVLVVEHDEDAIRCADYVV
                     DMGIGAGVHGGEVIAKGPLPDILKSRKSLTAKYLNGTLAIHVPKKRTRRDPGRQLVIS
                     GASGNNLKDVTLELPVGLLTCVTGVSGSGKSTLVNDTLYHAAARHLYGSQTEPAAYES
                     ITGLEHFDKVISVDQAPIGRTPRSNPATYTGVFTPIRELFSTVPTAKERGYSAGRFSF
                     NVKGGRCEACQGDGVLKVEMHFLPDVYVPCDVCHGKRYNRETLEVQYKGKSIHEVLEM
                     TVEEAFEFFKPVPVVARKLQTLLDVGLGYIRLGQSATTLSGGEAQRVKLSLELSKRDT
                     GRTLYILDEPTTGLHFHDIDLLLKVIHRLRDQGNTVVIIEHNLDVIKTADWLIDLGPE
                     GGAGGGEIIATGTPETVAAHPTSFTGKYLAPMLKK"
     gene            8666..9859
                     /locus_tag="VM406_14555"
     CDS             8666..9859
                     /locus_tag="VM406_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL77233.1"
                     /translation="MSAAEIRASASLASIFGLRMLGLFLVLPVLAVHAESMPGGGNTA
                     LIGLALGIYGLTQAFGQLPFGIASDKFGRKRVIIIGLVLFAIGSLIAAAATDLTWLIV
                     GRAVQGAGAISAAVTAFIADSTRDEHRTKAMAMVGASIGLTFAFSLVAAPMLYNRIGM
                     AGIFTLTAVLSLMAIAVVAWVIPAAPPKQAMPKVPMRELLRHGELMRLNYGVFALHVT
                     QLAMFVVVPAALVQHAQLPLPEHWKVYLPVVLVSFVLMLPPVFAAERQGRMKQLFLAS
                     IGLMLLVQLGFWLFAAAGDLHWGLLAVLLLLFFVSFNILEACLPSLVSRIAPASSKGA
                     ALGVYNTVQAFGLFCGGALGGWLAQYLGPSAVFALGAVLSVVWLIIAAGMRHLPRRGA
                     HSAQH"
     gene            9905..10387
                     /gene="ssb"
                     /locus_tag="VM406_14560"
     CDS             9905..10387
                     /gene="ssb"
                     /locus_tag="VM406_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HVL77234.1"
                     /translation="MASVNKVIIVGNLGRDPETRYMPSGDAITNIAVATTDRWKDKAT
                     GEQKEQTEWHRIAFFGKLAEIAGQYLKKGSQVYVEGRLRTRKWTDKDGAERYTTEIVA
                     DAMQMLGSRQGAGGSGAQMDDGYGGAPAPRQNAGGGGASRPASRPAPNFSDMDDDIPF
                     "
     gene            complement(10479..>11272)
                     /gene="speE"
                     /locus_tag="VM406_14565"
     CDS             complement(10479..>11272)
                     /gene="speE"
                     /locus_tag="VM406_14565"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HVL77235.1"
                     /translation="QRTACQHLELLESAELGRTLRLDGSFMTSEAEEFFYHEAMAHPA
                     AIAHPAPRNVLVIGGGDGGLAEELLKHPSVKDLTLVELDVEVVETARTWLQRIHRGAL
                     DDPRVKLRIEDGAAFIANTDQRFDLVLLDLTDPETPAGPLYTREFFARCREVLAPGGA
                     MVLHLGAPFYEPEQVRTFGAALSTVFSIVRGYGLHIPLYGAYWALAVVSDTLDPTALG
                     TDEVRQRLEERRIGALQYYNAEVHGALFALPNFYRALLVPGRNGA"
BASE COUNT         1787 a         3594 c         3698 g         2193 t
ORIGIN      
        1 agcttgcgca gcttgttgaa atcgcgctgg gcgaacaagg tcagatggtt ctcgacgatg
       61 ttgagcgcca tgcggcgcgt gatcaacggg atcttcggca gccggcggat ctgcagcgcc
      121 aggcactcgg acgcgttgcg cgcgccgatg ccggccggct cgaaactttg caccagcgtg
      181 agcgcgacac gcagctcatc caggtcaatg cccagttctt caggcagccg cgcatgaatt
      241 tcctccagcg gctcgtcaag gtagccgttg tcgttcaacg cgtcgatgat cagctccacc
      301 agggcccggt cacgcgactc gcgcacggtc aggcgcattt gatccagcag atgttcgcgc
      361 aaggtgatgc cgggcgcttc cagttgcggg cgtgcgtcgt cgtcgtccgg cgcctttgaa
      421 gttcgcgcga catcgtcgaa gctccaatcg ccctcggccc cggcgggcgc atccgggacg
      481 tccccgccat ccgcggcgcc gtttggctcc tccgcgccgc catcggaggc gagctggggg
      541 gcctccgcgc tcccgttgac ggcgggaccg gggctgattg cgccatcggc gaggaggcgc
      601 acggagtgat ccagcgggtc gtccatgcgc tcgagcaatg gattgtcgga aagaatctgc
      661 tcgagctcct ggtgcagctc cagcgtagac aattgcagca ggcgaatcga ttgctgcaac
      721 tgcggcgtca gagcgagatg ctgcgacatg cggagttgta gcgtctgttt catggctttc
      781 tgccttacat ccggaagtgt tcgccgagat agacccggcg cacggactcg ttgcggataa
      841 tgtcgtcggg gcgcccgctc gccaggacag cgccctggtt gatgatgtag gcgcggtcgc
      901 agatgcccag cgtttcacgc acattgtggt cggtgatcag cacgccgatg ccgcgttcct
      961 tcaggaatcg cacgatgcgc tggatttcga tcacggcaat cgggtcgact ccggcgaatg
     1021 gttcgtcgag cagcacgaag cgcggattgg tggccagcgc ccgcgcgatc tcgacacggc
     1081 ggcgttcgcc gcctgatagc gagagcgcct ggttctcgcg cagccgttcg atctggaggt
     1141 ctgccagcag cgcgttgagg cgtcgatcga tttcgtcgcg gccgagccgc ttgccgttct
     1201 tgtcaagctg gagttccagc accgcacgga tgttctcctc gaccgtcagc ttgcggaaca
     1261 ccgaagcttc ctgcggcagg taggacaggc cgagcatcgc gcgccggtgg atcggaagac
     1321 gcgagatcgc gtggtcatcg agatcgattt ctccggcgtc caggggcacc aggccgacga
     1381 tcatgtagaa cgaggtcgtc ttgcccgcgc cgttcggccc gaggagtccg accacttcac
     1441 cgctgctgac ctgcagcgag acgtcgtgga ccacctggcg cgagccgtag ctcttctgca
     1501 ggccgcgaac gaccagagta cttgtcgtca ttgggacctt gcctccgtgc gcggctgaat
     1561 cgtggcacgt atgcgcccgg cgccaggttt gctctcgccg ccggccgtgt tgttgaccgt
     1621 atagaactcc ttctggctgt cgtaggtgat cgacgcgccc tgcacctcgt cgatgggacg
     1681 ggtcccctcc agccggcgca tgttcgcgcg ccgtatcaga tgcacggttt cggccttgcc
     1741 gttgtattcg atgcgttcgg cttcgccttc cacccagtcg tcgggacccg agtcgcgctt
     1801 ctgccggaaa gtcgcaagtc cgcctggcgc tgcttcgagc gtggcgtact ggtagccctg
     1861 cgggtcttgg gacaccacca ggcggtgggc gcgcatgaca agtgatcctc gcgtgagcac
     1921 gacgtttccg gtgaaggtgt tgatctgctt gatgtcgtcg tagaccatcc gatcggcttc
     1981 gatgttggtc ggctgctcgg aatcggcacg ggcggcatgc gccgtgccgg ttgccatggc
     2041 cagcgcgacg agcagcatgt ggatcgtacg tttcatggaa tgtctcgacg gtgaatacaa
     2101 cttataactc gaactagcga ggcggtggtt gatacgtgcc atgcactctg ctgtgcagct
     2161 tgaattcgcg cgtggcgttg ttcgcgtaca tgccggttcc gaacagcgtt gatttcccgc
     2221 gagtgatctt caccggcttg tctgtctgca tcacgtcgtc gtccgggaac acgatcatgt
     2281 gctgcgagtg cagttgaagc cgttcctgcg aaccgctcgc cggacgatcc atgtgcacgt
     2341 tctcgtgcag gtggatctcg ctggtgtcgc tgttgatcca cgcgcgttct gcacgcaccg
     2401 tcatcggttc ctcgccaccg cgcagggcgc gcacgaccgg ctcctgcaca tcataggaat
     2461 cgtcgagcgg gttgtgggtc agccgggtgc ccgccacgtg gtactgcgca tcgccggtgc
     2521 gcgacatcct gacaaaattg aactgctcca cgtagaagtc cggctccgcg cgctgcccgc
     2581 gatcgatgaa atcgtccgcg ccgcggcgca tcacctccac gacccagaag ctggcaagcg
     2641 ctagggcaat gaccggcgcc agtatcgcca ccaggcgaaa gcggctttga tgacgcatcg
     2701 ctatccgagg tagggcgcca gcgcagcttc gtaactgccg cgcgccttca ggatgaagtc
     2761 gcacacctcg cgcaccgcgc cgtgcccgcc ggccgcggtc gtcacgtaat gcacgcggcc
     2821 gcgcacttcc ggatggccgt tcggcacgct tgcggcaaat gccacctgca gcagaatggg
     2881 caggtcgatc acgtcgtcgc cgatatagcc gcagtgggtg gcgtccatcc cggtttgtgc
     2941 aagcaggcgc gcgaaggcct cacgcttgtc gtgcgcgccc tggaagacat gggtgacgcc
     3001 gagatcggct gcgcggcgcg taacgatctc cgactcgcgc gcactgatga tggccgtcgt
     3061 cactcctgca cgctgcagca gcttcatgcc atgaccatca agaacgttga agcgcttgat
     3121 gtgctcccca tccttgccgt aatgcaggct tccatccgtc aacacgccgt cgacgtcgaa
     3181 gatcatcagc cgcacatcgg ccgcgcggga aacggtttgc tcgagcatgt cagatcacct
     3241 tcgcgcgggt aaggtcgtgg atgtgcagcg cgccgactaa ggtgccgtcc tcggccgtga
     3301 ccagcagctg gttgatgcga tatcgctcca tcagctcgac cgcgtcgacc gccagctgtt
     3361 ctggtcgcac cgtatgcggg ttgcggtgca tcacgtcgcc gatggtgacg gcgttcaagt
     3421 cttgcacgtt ctcgatcagg cgccgcaagt cgccgtcggt gaacacgcca agcacacgat
     3481 tcgctccgtc aacgatggcg gtcatcgcca ttcccttgcg cgtgacttcg agcagcgcat
     3541 gcggcagcgc aagctcgggc gtgacggacg gaaccgcatc gccgctgcgc atgacgtcgc
     3601 gtacgtgggt gagcaggcgt cggcccaagg cgccgcccgg atgcgagcgg gcgaagtcct
     3661 cctcgcgaaa gccgcgcgcg tcgagcaggg caaccgcaag cgcatcgccc agcgcgagcg
     3721 cggccgtggt gctggcagtc ggagcgagat tgagcgggca ggcttccttg gtcacgcgcg
     3781 cattgagatg gatgtcggcg agcctggcca ggcgtgagtc ctcattgcct gtgatcgcaa
     3841 tgagcttggc gcccatgcgg cggaggatcg ggatgatggc gagaaactcg gccgcttcgc
     3901 cggagttcga gatggcgatc aaggcgtcgt tttcggtaat catgccaagg tcgccgtgca
     3961 acgcttccgc cgcatggacg aacagcgccg gggtgcctgt cgaggcgagc gtcgcggcga
     4021 tcttgcgcgc tatatggccg gacttgccga tgccggaaac gacgacgcgt ccggaacatt
     4081 ggagcagcag ggcgacggcg tgggcaaacc ggttatcgtc gtcgagtctt gccttcaaat
     4141 gcaggatcgc gtcggcttcg atctggagcg tttcgcgcgc cagttccatc gcgcgcgagg
     4201 cggtgtcggg gagggacgca ggggacgtct ttgcttcgga tttagacatc cgcgcagtat
     4261 agcccaattg ggaaagcaag aaatctgcca atgcttgccg aatcgtcttg gacccgtcgg
     4321 ggaacggcgc gtgtttcggt gcgcggcaca gtggcggctt atgtgttgct gtcgcgcaat
     4381 tctcagaggg aagcggctac aatggcgcgc tcctcagccc gctcccgcca tggccctgtt
     4441 cgaacgcatt ctcggcatca tcctgcccgt ttttctgatc atcggcctcg ggtacgccta
     4501 tgcgcgctgg cgccccgacg ccgttcgaag cgacatggcg gcggtcaacc ggatcagcat
     4561 cggggtgctg tgtccgctgc tggtattcac cgcgctggct tcgaaggatt tcgatgtcgt
     4621 ccacaacgcc accctgattc tcgccggcgt cggcatcgcg ctgggctccg gcctgcttgc
     4681 ctggccgctc gcgcggatat tcggctacga cgtacgcact ttcgtgccgc cgatgatgta
     4741 caacaattgc gggaacatgg gcctgccgct ggccgtgctg gcattcggcg catcgggcct
     4801 gcccgccgca gtggcgatgt tcatggcctg caacctgatc tacttttcgg tcggcatacg
     4861 catcatcgag agcgggcgcg gggtgcatac cccgttctgg cagttccttg cgaacccgat
     4921 gatgatggcg atgctcgccg ggctggcatt tgcctttctg cgcgttccgc tgcccgagga
     4981 attgatgcgt gcgatgcgca tgctcggcga ggccagcatt ccgatcatgc tgtttgcact
     5041 cggcgtacgg atgctggacg tcagtctcaa cagctggcgc gtcgggttgg cgggggcggt
     5101 cgtgtgtccg gccgcgggac tggcggtggc ctggctgctt gacggcgtac tgactctgaa
     5161 cgaagcccag cgcgggcaga tgtttctgtt cgcggcgctg cctccggcgg tgttctgctt
     5221 catgatcgcc gagcagtacc ggcaggagcc ggacaaggtg gcgtccattg tcctgctcgg
     5281 caatctcgcg gcgctcggtt tcgtgccgct gggactgtgg atggggatgc gctgacgccc
     5341 gctacgccgt cggcagtacc gcaaatccct cgcccggcgg atcgtcgacc tcgctgagca
     5401 cgactgcgtc cacccgcgct gcgggcggtc cctcatgcgc ccagccgacc atctgcgcga
     5461 cggcatcttc gtcgccgtcg acgagcgcct cgaccgaccc gtcgcgccgg ttgcgtaccc
     5521 agccccgcag gcgcagcgcc cgcgcctgcg cctcgaagct ggcgcggtag cccacgccct
     5581 gcacccggcc ggtaatcttc agatgcctgg ccatcgcagt ccttattttt tcaacatcgg
     5641 tgccaggtac ttgccggtga agctggtcgg gtgcgccgcg accgtttccg gggtgccggt
     5701 cgcgataatc tcgccgccgc cggcgccgcc ttccggaccc aggtcgatca gccagtcggc
     5761 cgtcttgatg acatccaggt tgtgctcgat gatgaccacg gtgttgccct ggtcgcgcag
     5821 ccggtggatc accttcagca gcaggtcgat gtcgtggaaa tgcaggccgg tggtcggctc
     5881 gtcgaggatg tagagcgtgc ggccggtgtc gcgcttggac agttccagcg acagcttgac
     5941 ccgctgcgct tcaccgcccg agagggtggt cgcgctctga ccgagccgga tatagcccag
     6001 gcccacgtcc agcagcgtct gcagcttgcg cgcgacgacc ggaaccggct tgaagaactc
     6061 aaacgcctcc tcgaccgtca tctccagcac ttcgtggatg ctcttgccct tgtactgcac
     6121 ttccagcgtt tcgcggttgt agcgcttgcc gtggcagacg tcgcaaggca cgtagacgtc
     6181 cggcaggaag tgcatctcca ccttcagcac gccgtcgccc tggcaggctt cgcagcggcc
     6241 gcccttgacg ttgaacgaga agcgcccggc cgagtagccg cgctcctttg cggtcggcac
     6301 cgtggagaac aattcgcgga tcggcgtaaa cacgccggtg taggtggccg gattcgagcg
     6361 cggcgtgcgg ccgatcggcg cctggtcgac cgagatcacc ttgtcgaagt gttcgaggcc
     6421 ggtgatcgat tcgtaggccg ccggttcggt ctgggagccg tacaggtggc gcgctgccgc
     6481 gtggtacagg gtgtcgttga cgagcgtcga cttgccggag ccggacacgc cggtcacgca
     6541 ggtcagcaat ccgaccggca gttccagcgt cacgtccttc aggttgttgc cgctggcgcc
     6601 ggaaatcacc agctggcggc cgggatcgcg ccgggtgcgc ttcttcggga cgtggatcgc
     6661 cagcgtgccg ttcaggtact tggcggtcag cgacttcctg ctcttcagga tgtccggcag
     6721 cgggcccttg gcgatcactt cgccgccgtg cacgccggcg ccgatgccca tgtcgaccac
     6781 ataatccgcg cagcggatcg catcctcgtc atgctcgacc accagcacgc tgttgccgat
     6841 gtcgcgcagg tgtttcagcg tggcgatcag gcggtcgttg tcacgctggt gcaggccgat
     6901 cgagggttcg tcgagcacgt acatcacgcc ggtaaggccg gagccgatct gcgaagccag
     6961 tcggatgcgc tgcgcttcgc cgccggaaag cgtgtcggcg ctgcgttcga gcgacaggta
     7021 atcgaggccg acattgttca ggaacttcag gcgcgaaacg atttccttga tgatgcggtc
     7081 ggcaatctcc ttcttcgcgc cggtgagctt cagcgattcg aagaaggcca gcgcatcgcg
     7141 caacggcttg gcggcgatct cgtagattgc acgctgctgc ctgccgctgc cgatcttcac
     7201 atggcgcgcc tctacacgca ggcgcgcgcc atggcattcc gggcattgtt tctggttgat
     7261 gaacttggcc agctcctcgc gcaccgccat cgagtcggtc tcgcgatagc ggcgctgcag
     7321 gttgttcacc acgccttcga acgtatgctc gcgcaccacc gcccggccgc gttcgttgat
     7381 gtaggtgaag ggcacggcct ccttgccgga gccgtacagc acggcctgct gggccttgtc
     7441 cggcaggctg tcgaacggtt cgtcgagatc gaagccgtag tgctgcgcca ggctggagag
     7501 catctggaaa tagaattggt tgcgccggtc ccagcctttg atcgcgccgc tggccaatga
     7561 gaggttcggg aaggcgacga tgcgcttcgg atcgaagaat tcgatatggc cgagcccgtc
     7621 gcactcgggg caggcaccca tcggattgtt gaacgagaac aggcgcggct cgagttcctg
     7681 cagcgaatag ccgcagatcg cgcaggcgaa cttgttcgag tacaggtgct cgcggccgcc
     7741 gtccatctcg agcgcgatcg cgcggccgtc ggcaagccgc agcgccgtct cgaaactctc
     7801 ggcgagccgc tgcttgacat cggccttcac cttgagccgg tcgatcacca cgtcgatcgt
     7861 gtgcttctcg gtcttcttca gcttcggcag gtcgtcgacc tcatagatct tcgccgcctg
     7921 ggtgccgctc tggatgcgaa aacgcacgaa gccctgcgcc tgcatgtgct cgaacaggtc
     7981 cgcatgctcg cccttgcggt tggcgacgac aggggccagg atcatcagct tggtgtcctc
     8041 cggcaatgcc aggaccgcat cgaccatctg cgagacagac tgtgccgcca gcggatgctc
     8101 gggatgctgc gggcagtagg gcgtgcccac gcgcgcatac agcaggcgca ggtagtcgtg
     8161 gatttcggtg actgtgccca ctgtcgagcg cggattgtgc gaggtcgcct tctgttcgat
     8221 ggagatcgcc ggcgacaggc cctcgatcag gtcgacatcg ggtttctcca tcagctgcag
     8281 gaattgccgg gcgtacgccg acagcgattc cacataccgt cgctggcctt ccgcatacag
     8341 ggtatcgaag gcgagcgagg acttgcccga accggacaga cccgtgatga cgatcagctg
     8401 gttgcgggga agctcgaggt tgatgttctt caggttgtgc gtgcgtgcac cgcggatgcg
     8461 aatttcttcc atgaaattcc tggggttgga agcagtgatg ggaagctggc aacgcgcccg
     8521 cgccatcggc gggacgtggc agcttcgcga cggtcaacct gatactatag cgggttttca
     8581 agcccgccgt atccgcctgg ttgccctgcc cgtgtcccag ccgaccactc cttccgccgc
     8641 gtccgatctg cttccgagta cccgcatgag cgcggccgaa atccgcgcca gcgcgtcgct
     8701 tgcctccatc ttcggactgc gcatgctcgg gctgttcctg gtgctgccgg tgctggcagt
     8761 ccatgcggaa tcgatgccag ggggcggcaa cactgcgctg atcggcctcg ccctgggcat
     8821 ctatggcctg acgcaggctt tcggccagct gccattcggg atcgcatcgg acaagttcgg
     8881 gcgcaagcgc gtgatcatca tcgggctggt gttgtttgcc atcggatcgc tgatcgccgc
     8941 ggccgccacc gatctgacgt ggctgatcgt cggtcgggcc gtccagggag ccggcgcaat
     9001 ttcggcggcc gtcacggctt tcatcgccga ctcgacccgc gacgagcacc gcaccaaggc
     9061 catggcgatg gtcggcgcct cgatcggtct cacttttgca ttctcgctgg tcgccgcgcc
     9121 gatgctctac aaccgcatcg gtatggccgg catcttcacg ctgaccgcgg tgctttcgct
     9181 gatggcgatc gcagtcgtcg cctgggtcat accagccgcg ccgccgaagc aggcaatgcc
     9241 gaaagtgccg atgcgcgagc tgctgcggca tggcgaactg atgcggctga actacggcgt
     9301 gtttgcgttg catgtgaccc agctcgcgat gttcgtcgtg gtgccggccg cgctggtcca
     9361 gcatgcgcag ctgccgctgc ccgagcactg gaaggtgtat ctgcccgtgg tgctggtgtc
     9421 gttcgtgctg atgctgccgc cggtgttcgc ggccgagcgc cagggaagaa tgaaacaact
     9481 tttcctggcc tcgatcgggc tgatgctgct ggtgcagctc gggttctggt tgttcgccgc
     9541 agcaggcgac ctgcattggg gactgctcgc ggtgctactg cttctgttct tcgtcagctt
     9601 caatatactg gaggcctgtc tgccctccct ggtgtcgcgg attgcgcccg cttccagcaa
     9661 gggtgcggca ctgggcgtct acaacacggt tcaggcattc ggcctgttct gcggcggcgc
     9721 gctgggtggc tggctcgcgc agtacctggg gccgtccgcg gtcttcgcgc tcggcgcggt
     9781 gctcagtgtt gtctggctta taattgccgc cggcatgcgc caccttcccc ggcgcggcgc
     9841 ccattctgcg cagcattgat tcggcgacca ccctttatcc cccgttttag ccctaggaga
     9901 aatcatggca tccgttaaca aggtcatcat cgtcggcaac ctcgggcgcg acccggaaac
     9961 gcgctacatg cccagcgggg atgccatcac gaatatcgcc gtcgcgacca ccgaccgctg
    10021 gaaggacaag gccaccggcg agcagaaaga gcagaccgaa tggcaccgta tcgccttctt
    10081 cggcaagctc gccgagatcg cgggccagta tctgaagaag ggctcgcagg tgtacgtgga
    10141 aggccggctg cgcacgcgca agtggaccga caaggacggc gccgaacgtt acaccaccga
    10201 gatcgtggcc gacgctatgc agatgctcgg cagccgccag ggtgcgggcg gcagtggcgc
    10261 gcagatggat gacggctacg gcggcgcacc ggccccgcga cagaatgccg gcggcggcgg
    10321 cgccagccgc ccggcttcgc gtcccgcgcc gaacttcagc gacatggacg acgatatccc
    10381 gttctgacaa atacaccggc ggcagtgcgc ggggaggcct tcaagcctcc ccgttttttt
    10441 cgcccctgct gcgtgcgtcc cgcagttgtg cgctggtcct atgcgccgtt tcggccgggg
    10501 acgaggagcg ccctgtagaa gtttggcaac gcaaacagcg cgccatggac ttcggcgttg
    10561 tagtactgca gcgcgccgat acgccgttcc tccagccgtt gtcggacctc gtccgtaccg
    10621 agggcggttg gatccagcgt gtcggagacc acggcgagcg cccagtaggc gccgtaaagt
    10681 ggaatgtgca gcccgtagcc gcgtacgatg gaaaacaccg tcgacagcgc cgcgccgaac
    10741 gtgcgcacct gctccggctc gtaaaacggc gcacccagat gcaggaccat cgcccctccc
    10801 ggtgcgagca cctccctgca gcgcgcaaag aattcacgcg tgtagagcgg gccggcgggc
    10861 gtctccggat cggtcaggtc gagcaggacc agatcgaaac gctggtcggt gttcgcgatg
    10921 aacgctgcgc cgtcctcgat ccgcagcttc acgcgcggat cgtcgagcgc cccgcgatgg
    10981 atgcgctgca gccaggtgcg cgccgtttcg accacttcca catccagttc gaccagggtc
    11041 agatccttca cgctcgggtg cttgagcagt tcttctgcga gaccgccatc gccgccgccg
    11101 atcaccagca cgttgcgcgg ggccggatgc gcgatcgccg ccgggtgggc catcgcctcg
    11161 tggtagaaga attcttccgc ttctgaagtc atgaagctgc cgtccaggcg gagcgtgcgg
    11221 cccagctccg cggattccag caactccagg tgctggcagg cggtgcgctg tg
//
LOCUS       DATLGF010000470        10091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_13506,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000470 DATLGF010000000
VERSION     DATLGF010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 10091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10091
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13506"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VM406_14570"
     CDS             <1..260
                     /locus_tag="VM406_14570"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVL77236.1"
                     /translation="YRLVNHVTESGKALDKARELAGAICAAAPLPVMFIKSSVDSGLD
                     MTRADAMSAEGDASFMLYFSKDRDEGLAAFKEKRTPKFRGE"
     gene            268..1434
                     /locus_tag="VM406_14575"
     CDS             268..1434
                     /locus_tag="VM406_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011178401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HVL77237.1"
                     /translation="MKKKDIVIAGYAETAIDYKTGRSAYDLAGEALSQLLERTGIDKD
                     RIDGLSVTTALSEGSNPFYAVYMCEALGLTPSWLNLGGIGGCSATGGIARAMSAIRDG
                     ICETVVVLSADAPSTDWRANYGAFRGEFQDPPGVQGPPATFGLLMSRYIHQYGLDPAA
                     LGKIAITQRNHALHNENAYKKFKKPLTMDEYLASRVVADPLRLLDSVMFCDGANAFLV
                     TTEKNAQKLGINKVVYPVAYGEVTNLNGNQATPDITISGFDRIGPALLDKAGLKPADI
                     DMFQPYDDFTIAVMMQFEAFGFCRKGEGSAFTLRTDLSHTGELPLNTGGGQISAGQPG
                     LASGGLNLAEAVRQLFGEGGARQVADARNALVTGIGVIPYARNWGTSSAMILEV"
     gene            1435..1875
                     /locus_tag="VM406_14580"
     CDS             1435..1875
                     /locus_tag="VM406_14580"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HVL77238.1"
                     /translation="MSEQATQAAAAAAAKRPLPREGAYVDTRPFWEGVAQNRLLLQYC
                     TVAQKFQHYPRPVSIYTGRRTLEWREVSGDGVIYAFTTVRIPGPGLEGRLPLCVATVE
                     LDEGVRMIANILDCAPEDLAIGKRVKLAWDLLSEDKKYPAFKLV"
     gene            complement(2229..3167)
                     /locus_tag="VM406_14585"
     CDS             complement(2229..3167)
                     /locus_tag="VM406_14585"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVL77239.1"
                     /translation="MHERLDLNSLRLYYDVVNAGSITRASEQLRMPKSTISRKLAQLE
                     NDLGVILLRKGPKRLSTTDIGAALYDRCRRLVIEIEDVGLATAEMQGELRGVLRVSIP
                     VDFGISWISRTIAAFVRAYPEINLEIEVNSRAVNPREDPYDLTIQLGPLKESGLTYRR
                     LATITRGVYASPDYLARHGTPRTVEDFGKHDCIVTDQQRQDGVWTFRNQTRHRFIEVF
                     GKVMVNNIVIARDLAVAGVGLSMLPNIMCQNDVKAGRLVRVLTNWESPPVQATALILS
                     RKGIPNKTRVFLDFLADQLIDDARATGLQAEASVGK"
     gene            3565..4377
                     /locus_tag="VM406_14590"
     CDS             3565..4377
                     /locus_tag="VM406_14590"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVL77240.1"
                     /translation="MSKPLDKPGIKLAAKPAEGSAGRRELIALAVVIAGLALAMEIGS
                     RFTEDYILPSPVVVGRALIDLLTVDYMHVVITLGRLGMAIVFSLIVGVTIGLLMGTSK
                     KAGPYLKSLVIIDTGIPALSWMLIAVFWFKDPEARIFFILTVILLPFYALNVYEGLRA
                     IPRDWVDMMESFRPSRLQVMRYLIVPHIVPYIFLTTKSVIGYAIRMVIFAELVASAVG
                     IGSRMSFAQSTFRIDQVMAWTFLLVILNLALQSAANLAEKRLLKWRAEAKVR"
     gene            4377..5153
                     /locus_tag="VM406_14595"
     CDS             4377..5153
                     /locus_tag="VM406_14595"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL77241.1"
                     /translation="MTNASQPFISLKNVYKQFGDYVAVDNLSMEVHRGEIVALLGRTG
                     AGKSTVMSMLMGTIACDAGEVRVAGVDPCQEFDALRGKMAVSFQTDRLLPWRTAVENV
                     ELGLQILNRPKAETRRAALAWLERVKLKGTEDKYVHELSGGMRQRVSLARALAVDPEL
                     VFLDESFSQLDHVTSQVLRKDFAQIVRTYGKTCVFVTHRIEDALDIADRIFVLAAPAR
                     VCLELQVDAQLRADADVMSRLHDDIAHAIGGEEQEEGVHE"
     gene            5207..6757
                     /locus_tag="VM406_14600"
     CDS             5207..6757
                     /locus_tag="VM406_14600"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVL77242.1"
                     /translation="MRQEKLLAAHALRTPEKTALVTGGRRISYVELHESAQAMAAGLH
                     HCGLQPGDRVLVYLPNGLEFIQTVYAAFSVGAIVVPVNTRLTAKEVAYFAADSRPALM
                     VFHSDHADALAQVSDELAAIPKIVVGERVPGARRFEDVAAPSDEPLPEVPLQAEDCMI
                     MYTSGTTGKPKGAVLTHANFIVQHAYLNAIEWGIGASDRFLVTTPLAHRTGLARMMNA
                     LCLGATLVVMERFDPLAAIDLIEREQVTALGMVPTVARMLLPHLEQAPKRCASLRHVI
                     VTGEAFPVELKRRLIELLPAVRLHSFFAMTEVGSVSSLEHEEQFTHPTSVGRPTPGIE
                     VKLVDDKGREVSVGDAGEMLVRAGIPGRFTTMRGYYNRPEETAATIVDGWVHTGDMAR
                     FDADGYLYIVDRKKDMVLSGGFNIYTKEVEQALLEHEAVLDVAVVGAPDPVYGEAVVA
                     YVETRPGCSVTREALAEHTKSRIASYKKPKHVFFVDSLPRNPLGKILKNELRERAAVA
                     VRTEEPAC"
     gene            6751..7923
                     /locus_tag="VM406_14605"
     CDS             6751..7923
                     /locus_tag="VM406_14605"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVL77243.1"
                     /translation="MLRPESAEDARLRAEVAQWLREHVPAHLRHRTFRPEPAEALAWY
                     RKLSARGWIAPHWPRAWGGMEATPVQQVILMEEFARAGAPDLPTQGLNHIGPILIKCG
                     SDAQRARHLPAILNGEVIWCQGYSEPQAGSDLASLRTRAALQGGDLVINGHKIWTTWG
                     QHAHWMFALVRTGEGSGRQEGISFVLIDLSSPGITRRPITTIAGDDEFCEVFFDDVRV
                     PLENVVGELGQGWKVATALLSEERLRIGSPALALKALERLRMMASDERIGAARRPEFQ
                     ERLALAEIEVETLIASYLSVAESEAQAEGGRPDSSYLKILATETVQCILDSLQEIAGE
                     AAALARPVASGDALLDFSEMFLQSRRLSIYGGTNEIQRTILATRVLGLPVARRSRA"
     gene            7920..8972
                     /locus_tag="VM406_14610"
     CDS             7920..8972
                     /locus_tag="VM406_14610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVL77244.1"
                     /translation="MTSVQAMNEANLVAQSALRFAQGAGGPARARAVRDNFPQLDRAA
                     WRQIAEQGWLAMLVPEAAGGLGMDAAAACAVLEAAGASLLPEPVVPAMAAASFLGGIG
                     EPGADLLDTLLDASRVVLAVPAPMQPVFPAHLSHVADCYAGAVLLVAAEDDATGDGFE
                     VRAIALDAPGVRVDTAACVDGSLLSTLEIDGGAWRDAPRLAAGSRARDAWSGVRDVML
                     LGHAAALVGVMDAALEAAVGYMKVREQFGAPIGSFQALQHAAASAHVDVVATRALVRE
                     ACRAFNTPVQARAACAAKARASAASLRVTKACVQFHGAIGFADEHDIGLYLRRAMTLA
                     ARMGGQLQQSLRWRRL"
     gene            9627..>10091
                     /locus_tag="VM406_14615"
     CDS             9627..>10091
                     /locus_tag="VM406_14615"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HVL77245.1"
                     /translation="MNPPAAAVSDLRVWLSRLTETGRLAVIRENVALEHELAAIAKRL
                     DGKQAAFFPHPGGHPMPVISGFMSRRAWIAEAMGVPESGLLQAFRNAADNPLPWREVP
                     AEEAPCQQVVHHEIDLHGLLPIPTHSEHDNGPYITAGMVIARNPKTGVQNVSI"
BASE COUNT         1742 a         3229 c         3426 g         1694 t
ORIGIN      
        1 aataccggct ggtcaaccac gtcaccgaga gcggcaaggc gctggacaag gcgcgcgaac
       61 tggccggcgc gatctgcgcg gcggcacctt tgccggtgat gttcatcaag tcgtcggtcg
      121 attccggcct ggacatgacg cgcgccgatg cgatgtccgc cgaaggcgac gcctcgttca
      181 tgctgtattt cagcaaggac agggacgagg gcctggcggc gttcaaggaa aaacggaccc
      241 cgaagtttcg aggagagtga ggcagagatg aagaagaagg atatcgtcat cgccggttat
      301 gcggaaacgg cgatcgacta caagaccggg cgcagcgcct acgatcttgc cggtgaggca
      361 ctgtcgcagc tgctcgaacg caccggcatc gacaaggacc gcatcgacgg gctgtcagtg
      421 acgacggcgt tgagcgaagg atcgaatccg ttctatgcgg tctacatgtg cgaggcgctc
      481 gggctgacgc cgagctggct gaaccttggc ggcatcggcg gctgctcggc caccggcggc
      541 atcgcgcggg cgatgtcggc cattcgcgac ggcatctgcg aaacggtggt cgtgctgtcg
      601 gccgatgcgc cgagcaccga ctggcgcgcg aactacggcg cgttccgcgg cgagttccag
      661 gacccgccgg gcgtgcaggg accgccggcc accttcgggc tcctgatgag ccgctacatc
      721 caccagtacg ggctcgatcc ggctgcgctg ggcaagatcg cgatcacgca gcgcaatcac
      781 gcgctgcata acgagaatgc gtacaagaaa ttcaagaagc cgctcacgat ggacgagtac
      841 ctcgcttcgc gcgtggttgc cgacccgctg cgcctcttgg acagcgtgat gttctgcgac
      901 ggcgccaatg ccttcctggt cacgaccgag aagaacgcgc agaagctcgg catcaacaag
      961 gtcgtgtatc cggtcgcata cggcgaggtc accaacctga acggcaacca ggccacgccg
     1021 gacatcacca tatccggctt cgatcgcatc ggcccggcgc tgctcgacaa ggccggcctg
     1081 aagccggcgg acatcgacat gttccagccc tacgacgact tcaccatcgc cgtgatgatg
     1141 cagttcgaag ccttcggttt ctgccgcaag ggcgagggct cggcgttcac gctgcgcacc
     1201 gacctgtcgc ataccggcga gctgccgctc aataccggcg gcggtcagat ctcggccggt
     1261 cagccgggct tggccagcgg cggactgaac ctggccgagg cggtgcgcca gctgttcggt
     1321 gaaggcggtg cgcgccaggt cgccgatgcg cgcaatgcgc tggtcaccgg catcggcgtg
     1381 attccgtatg caaggaactg gggtaccagc tccgcgatga tcctggaggt ttgaatgtct
     1441 gaacaagcga ctcaggcggc cgcggccgcc gcagccaagc gtcccttgcc gcgcgaggga
     1501 gcgtatgtgg atacccgtcc tttctgggag ggcgtggcgc agaaccggtt gctgctgcag
     1561 tactgcacgg tggcgcagaa gtttcagcac tacccgcggc ctgtcagcat ctacacgggc
     1621 cggcgtacgc tggaatggcg cgaagtgtcc ggcgacgggg tgatctatgc gttcaccacc
     1681 gtgcgcatcc cgggaccggg gctggaaggg cgcctgccgc tgtgcgtggc gacggtggag
     1741 ctcgacgaag gcgtgcggat gatcgccaac atcctcgact gcgcgccgga ggatcttgcg
     1801 atcggcaagc gcgtcaagct ggcctgggac ctgctctcgg aagacaagaa gtatccggcg
     1861 ttcaagctgg tctgagcgag cacccccgcg tactggggga tcccctggca agcgaggacg
     1921 cagggcaagc tgacaagatt cctcgcttca attggaacga cggcattggg gtgtcatcgc
     1981 caaattactg tcgtcccgaa cgtagtgagg gcggcccgcg ttagggacct catcgcgctg
     2041 gcaagcgagg acgcagagca agtggatgag gttcctcgct tcgctcgaaa cgacggcatt
     2101 tggggccatc gctgattact gtcgacggca ttggaccatc gtccgatcat tgtcgtcctg
     2161 aatgcagtga gtcttccggc cacgagtgcg cgcaggcggg agcgacatcg acatcacacc
     2221 gttgatcgct attttccaac agacgcctcc gcctgcagcc ccgtcgcacg cgcatcatcg
     2281 atcaactgat ccgcgagaaa atcgaggaac acccgcgtct tgttcggaat gcccttgcgg
     2341 ctgaggatca gcgcagtcgc ctgcaccggc gggctctccc agttcgtcag cacgcgcacc
     2401 aggcgcccgg ccttcacatc gttctggcac atgatgtttg gcagcatcga caggcccacg
     2461 ccggccacgg cgaggtcgcg cgcgatcacg atgttgttca ccatgacctt gccgaacact
     2521 tcgatgaagc ggtgcctggt ctggttgcgg aaggtccaca cgccgtcctg gcgctgctgg
     2581 tcggtcacga tgcagtcatg cttgccgaag tcttccaccg tgcgcggcgt gccgtggcgc
     2641 gcgaggtagt ccgggctcgc atagacgccg cgcgtgatgg tcgcgagccg gcgatacgtc
     2701 agccccgatt ccttcagcgg gccgagctgt atcgtgaggt cgtacgggtc ttcgcgcggg
     2761 ttcacggcgc ggctgttgac ctcgatctcg agattgattt ccgggtaggc gcgcacgaag
     2821 gccgcgatcg tgcggctgat ccaggaaatg ccgaagtcga ccggtatgct cacgcgcagc
     2881 accccgcgca gctcgccctg catctcggcc gtcgccaggc cgacatcctc gatctcgatc
     2941 accagtcggc ggcaacggtc gtagagcgcc gcgccgatgt cggtggtgga aagacgttta
     3001 ggccccttgc gcagcaggat cacgccgaga tcgttctcga gctgcgcgag cttgcggctg
     3061 atggtggatt tcggcatgcg cagctgctct gacgcgcgcg tgatgctgcc ggcgttgacc
     3121 acgtcgtagt agagacgcag ggaattcaga tcgagtcgtt cgtgcatgga tgccgccgta
     3181 ctgcgatagt gttccattat tggaacggag gattctgttt tggattctat tcgatgcggg
     3241 agcgtttgag taacatgagt tcaaaccaat aagaagaagc aaagcgccgc tttcacgttt
     3301 tttcgttcgc agggatgcga gcgggatgac aggaacgaag agccagaaca ggagaccgcc
     3361 atgccccaac ccacgtccga cttccctcgc tacaccagaa cgcgagcgcg gctatagcgc
     3421 aagcgcgcgc cggcttgcgc acttcttgat tttcgtcaac ctgacgctgc cgcggaccgc
     3481 ttccggttcg tgtatgcgtt gcttgccgct gcgcgccgcg cggcgcgaaa cctggagcat
     3541 gaagtgaaga ctgcaatgaa tgcgatgtcc aaacccctgg acaaacccgg catcaagctc
     3601 gcggccaagc cggcggaagg atcggccggc cggcgcgagc tgatcgccct cgccgtggtc
     3661 atcgccggac tggcgctggc gatggagatc ggcagccgtt tcaccgagga ttacatcctg
     3721 ccgtcgccgg tcgtggtcgg cagggcgctg atcgatctgc tgacggtcga ttacatgcac
     3781 gtggtgatca cgcttggccg cctcggcatg gcgatcgtgt tctcgctcat cgtcggcgtg
     3841 accatcgggc tgttgatggg cacgtcgaaa aaggccggac cctatctgaa gtccctggtc
     3901 atcatcgata ccggcattcc ggcgctgtcc tggatgctga tcgcggtgtt ctggttcaag
     3961 gacccggagg cgcgcatctt cttcatcctg accgtgatcc tgctgccgtt ctacgcgctg
     4021 aacgtatatg aaggcctgcg tgcgattccg cgcgactggg tcgacatgat ggagagcttc
     4081 cggccctcgc gcctgcaggt gatgcgctac ctgatcgtgc cgcacatcgt gccctatatc
     4141 tttctcacca ccaagtcggt catcggttac gcgatccgca tggtgatctt cgccgagctg
     4201 gtcgcgtccg cggtcggcat cggttcccgc atgagctttg ctcaatccac tttccgcatc
     4261 gatcaggtga tggcatggac cttcctattg gttattctca acttggcgct gcagtcggcc
     4321 gcgaacctcg cggagaagcg gctgttgaag tggcgcgcgg aagcgaaggt gcgctgatga
     4381 cgaacgcttc ccagcccttc atctcgctca agaacgtcta caagcagttt ggcgactacg
     4441 tcgccgtcga taacctgagc atggaggtgc accgcggcga gatcgtcgcg ctgctcgggc
     4501 gcaccggcgc cggcaagagc accgtgatga gcatgctgat gggcaccatc gcctgcgacg
     4561 cgggcgaggt gcgtgtcgcc ggcgtggacc cgtgccagga gtttgatgcg ctgcgcggca
     4621 agatggccgt cagcttccag accgatcgcc tgctcccctg gcgcacggcg gtcgagaacg
     4681 tcgagctcgg cctgcagatc ctgaaccggc cgaaggccga gacgcggcgc gcggcgcttg
     4741 catggctgga gcgggtcaag ctcaagggca cggaggacaa gtacgtgcac gagctgtccg
     4801 gcggcatgcg ccagcgcgtg tctctggcgc gcgcgctggc ggtggaccct gagctggtgt
     4861 ttctcgacga atcgttcagc cagctcgatc acgtgacgtc ccaggtgctg cgcaaggact
     4921 tcgcccagat cgtgcgcacc tacggcaaga cctgcgtgtt cgtcacgcac cgcatcgagg
     4981 atgcgctcga catcgccgac cggatcttcg tgcttgcggc gccggcacgg gtgtgcctgg
     5041 aactgcaggt ggatgcacag ctgcgcgccg atgcggatgt gatgtcgcgc ctgcacgacg
     5101 acatcgccca tgcgatcggc ggcgaggaac aggaagaggg cgtgcacgag tgagcccgcc
     5161 gcgaagccgg cgcttcgcga acactttcag cgaacaagag gtatccatgc gacaggagaa
     5221 actgctggct gcgcacgcgc tgcgcacgcc ggaaaagacg gcgctcgtca ccggcggccg
     5281 ccgcatcagc tacgtggaac tgcacgaatc ggcgcaggcc atggcggcgg gcctgcacca
     5341 ctgcggcctg cagccgggcg accgcgtgct ggtctatctg cccaacggcc tggaattcat
     5401 ccagacggtg tacgccgcat tctcggtcgg cgcgatcgtg gtgccggtca acacgcggct
     5461 gacggcgaag gaagtcgcct atttcgccgc cgacagcagg ccggcgctga tggtgtttca
     5521 ttccgaccat gcggacgcgc tcgcgcaagt gtccgatgag ctggccgcga tcccgaagat
     5581 cgtcgtgggg gagcgcgttc ccggggcgcg ccggttcgag gacgtggcgg cgcccagcga
     5641 cgagccgctg cccgaagttc cgctgcaggc cgaggactgc atgatcatgt acacctccgg
     5701 caccaccggc aagccgaagg gggcagtcct cacccacgcc aacttcatcg tccagcacgc
     5761 gtatctgaat gcaatcgaat ggggcatcgg cgcgtccgac cgctttctcg tcaccacgcc
     5821 gctggcgcat cgcaccggcc tggcgcgcat gatgaacgcg ctgtgcctgg gcgcaacgct
     5881 ggtcgtgatg gaacgcttcg atccgcttgc cgcgatcgac ctgatcgagc gcgaacaggt
     5941 gaccgccctg ggcatggtcc cgacggtcgc gcgcatgctg ctgccgcacc tggagcaggc
     6001 gccgaagcgt tgcgccagcc tgcgccatgt gatcgtcacc ggcgaggcgt ttccggtcga
     6061 gctgaagcgc cgcctgatcg agctgttgcc cgcggtgcgc ctgcattcgt tctttgcgat
     6121 gaccgaggtc ggctcggtca gcagcctgga acacgaggaa cagttcaccc atccgacctc
     6181 ggtcggtcgc cccacgccgg gcatcgaagt gaagctcgtg gacgacaagg ggagggaggt
     6241 gtcggtcggc gacgccggcg agatgctggt gcgcgccggc attccggggc gcttcacgac
     6301 gatgcgcggc tactacaacc ggcccgagga aaccgccgca accatcgtcg acggctgggt
     6361 gcatacgggc gacatggcgc gcttcgacgc cgacggttac ctgtacatcg tggaccgcaa
     6421 gaaggacatg gtcctgagcg gcggcttcaa catctacacg aaggaagtcg agcaggcgct
     6481 gctcgagcat gaggcggtgc tggacgttgc cgtcgtcggc gcgccggacc cggtgtacgg
     6541 agaggcggtg gtcgcctatg tcgagacgcg gccgggctgc agcgtgacca gggaagcgct
     6601 cgccgagcac acgaagtcgc gcatcgccag ctacaagaag ccgaagcacg tgttcttcgt
     6661 ggacagcctg ccgcgcaatc cgctgggcaa gatcctgaag aacgagctga gggagcgcgc
     6721 ggcggtggcg gtgcgtaccg aggagccggc atgctgagac cggaatcggc cgaggatgcg
     6781 cgcctgcgcg cggaagttgc gcaatggctg cgcgagcatg tgcccgcgca tctgcgccat
     6841 cgcacgttcc ggccggagcc ggccgaggca ctggcctggt accggaagct gtccgcgcgt
     6901 ggctggatcg cgccgcactg gccgcgcgcc tggggcggca tggaagccac gcccgtccag
     6961 caggtgatcc tgatggagga gttcgcccgc gccggcgcgc cggaccttcc aacccagggc
     7021 ctcaaccaca tcggcccgat cctgatcaaa tgcggctcgg acgcgcagcg cgcgcggcac
     7081 ttgccggcaa tcctgaacgg cgaggtgatc tggtgccagg gctattcgga gccgcaggcc
     7141 gggtccgacc tggccagcct gcgcacgcgc gcggcgctgc aaggcggcga cctggtgatc
     7201 aacggacaca agatctggac cacctggggg cagcacgcgc attggatgtt cgcgctggtg
     7261 cgcaccggcg agggaagcgg ccggcaggaa ggcatcagct tcgtgctgat cgatctgtcg
     7321 tcgcccggca tcacgcgccg gccgatcacg accatcgccg gcgacgacga gttctgcgag
     7381 gtgttcttcg acgacgtgcg cgtgccgctg gagaacgtgg tcggggaact cggccagggc
     7441 tggaaggtgg cgaccgcgct gttgagcgag gagcggctgc gcatcggttc tcccgcgctg
     7501 gcgctcaagg cgctcgaacg gctgcggatg atggcgagtg acgagcgtat cggcgccgcg
     7561 cgccggcccg agttccagga gcggcttgcg cttgccgaga tcgaggtcga gaccctgatc
     7621 gcctcctacc tgagcgtggc cgaaagcgag gcacaggcgg agggcgggcg gcccgattcg
     7681 tcctacctga agatcctcgc gaccgaaacg gtgcagtgca tcctcgacag cctgcaggag
     7741 atcgccggcg aagcggcggc gctggcgcgt ccggtcgcca gcggcgacgc gttgctcgat
     7801 ttttcagaga tgttcctgca gtcgcgccgg ctgtcgatct atggcggcac caacgagatc
     7861 cagcgcacga tactcgccac ccgggtgctc ggcctgccgg tggcgcgcag gagccgggca
     7921 tgacatccgt acaggcgatg aacgaggcga acctggttgc gcagagtgcg ctgcggttcg
     7981 cgcagggagc cggcgggccg gcgcgggcgc gcgcggtgcg cgacaacttt ccgcagctgg
     8041 accgcgccgc ctggcggcag atcgccgaac agggatggct ggcgatgctg gtgccggagg
     8101 cggccggcgg tctcgggatg gacgcggccg ccgcctgcgc ggtgctggaa gccgccggcg
     8161 ccagcctgct gccggagccg gtcgtgccgg caatggcggc ggcatcgttc ctgggcggca
     8221 tcggcgagcc cggagccgat ctgctcgata cgctgcttga cgccagtcgc gtcgtgctcg
     8281 cggtaccggc gccgatgcag ccggtctttc ccgcgcacct gtcgcatgtg gccgactgct
     8341 atgcgggtgc ggtgctgctg gtcgccgcgg aagacgatgc aaccggggac ggcttcgagg
     8401 tgcgagccat cgcgctcgac gcgcccgggg tgagggtcga caccgcggcc tgcgtcgacg
     8461 gcagcctgct gtccacgctt gagatcgacg gcggcgcctg gcgcgatgca ccgcggctgg
     8521 ccgccggctc ccgagcgcgc gacgcatgga gcggggtccg cgatgtcatg ctgctcggcc
     8581 atgcggcggc cctggtcggc gtgatggacg cggcgctgga ggcggccgtc ggctacatga
     8641 aggtgcgcga gcagttcggc gcgcccatcg gcagcttcca ggcgctccag cacgctgcgg
     8701 ccagcgccca tgtggacgtg gtcgccaccc gcgcactggt gcgcgaggct tgccgggctt
     8761 tcaacactcc ggtccaggca cgggccgcct gcgcggcgaa agcgcgcgcc tcggccgcct
     8821 cgctgcgggt gaccaaggcc tgcgtccagt tccacggcgc gatcggcttc gccgacgagc
     8881 acgacatcgg cctgtacctg cggcgggcga tgaccctcgc cgcgcgcatg ggcgggcagc
     8941 tgcagcagag cctgcgatgg cggcggctct gagctgaagg gcgttttctg cgacggcatt
     9001 tgggtgccat cgcctgatca cgtcgtcccg aacgcagtga gggcagcccg cgtcaggaat
     9061 ctcatccgct tggcaagcga cggcgcgtag caagcggatg agcttcctcg cttcgctcgg
     9121 aacgacaggg tgggggaccg tcgccaaaat gactgtcgtc ccgagcgaag cgagggacct
     9181 catcgccctg gcacgcgatc ggcatggccg gcgccggcaa gccccgcaac gtgcggcgcc
     9241 actggccgcg caccatcact ttggatgatg tacgccggcc cacagtcctc ccggatcccc
     9301 tcaagcgcgc tcaccgcctc gctcattgat ccccgacatt cccaacggca tcatccgccc
     9361 aacgcatcat aaagaatgat ttattgaatc attaaatatt atttgtattg attaatagcg
     9421 ctccctatac tttggaacgc aatacaaggc aatccgcagg cgccgcagca gcggccgctg
     9481 cggcaaggcg gtcgctaccg gcgccgcacg ccgacaagaa caggagactt cagcatgacc
     9541 acatccgctt gcctggccag gtgcaccgct acggagcagc cgctgtccgg cgacgaatct
     9601 tcccgaaagg tgtccgaatg aatgcaatga atccacccgc cgccgcggtg agcgacctgc
     9661 gcgtctggct gtcgcgcctg accgagaccg gccggctggc cgtcatccgc gagaacgtgg
     9721 cactggaaca cgagctggcc gcgatagcca agcgcctgga cggcaagcag gcggcgtttt
     9781 tcccacaccc cggaggacat ccgatgccgg tgatttccgg tttcatgtcg cgccgcgcct
     9841 ggatcgccga agcgatgggc gtgccggaat ccggactgct gcaggccttc cgcaacgccg
     9901 ccgacaatcc gctgccctgg cgtgaagtgc cggcagaaga ggcgccgtgc cagcaggtgg
     9961 tgcatcacga gatcgacctg catggcctgc tgccgatccc gacccacagc gaacacgaca
    10021 acggccccta tatcacggcc ggcatggtga tcgcgcgcaa tccgaagacc ggcgtgcaga
    10081 acgtgtcgat c
//
LOCUS       DATLGF010000471         7493 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_185203, whole genome shotgun sequence.
ACCESSION   DATLGF010000471 DATLGF010000000
VERSION     DATLGF010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 7493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7493
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185203"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..91)
                     /locus_tag="VM406_14620"
     CDS             complement(<1..91)
                     /locus_tag="VM406_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HVL77246.1"
                     /translation="MSTELNLQGKTALVTGASSGLGARFASVLA"
     gene            264..1946
                     /locus_tag="VM406_14625"
     CDS             264..1946
                     /locus_tag="VM406_14625"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HVL77247.1"
                     /translation="MILTQDPRFLEQYGPRESMEYDVVIVGGGPAGLATAIRLKQINS
                     DISVVVLEKGSEPGAHILSGAVMDPRAMNELFPNWKELGAPLNQPVTGDDLLTLSETG
                     VRRVPDWLMPRNFHNDGCYVISLSNVVKWMAQQAEGLGVEIFPGFSAAEVLYDEQGRV
                     KGVATGNMGIEKNGEPGPNFQLGMELLGKYTIFAEGARGHLGKQLIAKFKLDEGKDPQ
                     SFAIGIKELWEIDPAKAKPGLVVHTAGWPIEDDTFGGGFLYHLEDNKVTLGYVIGLDY
                     QNPYMSPFEEMQRWKLHPEIRKHIEGGKRLGYGARAINNGSPQALPKTVFAGGALVGC
                     DAGYMNAARIKGSHAAIKSGMLCAEALADALAAGRSLDELSAYPEAFEKSWLNEELQQ
                     TRNFKLWFKKGNTMGQLMTGVEQWFLPKIGIASPPWTLHRDKPDHVYLKPADQCRPID
                     YPKPDGQITFDRLSSVFVSNTNHEENQPSHLTLKDEGIPVEVNLNKYAGPESRYCPAG
                     VYEFVKADDGRERLQINAQNCVHCKTCDIKDPTQNIVWVTPEGGGGPNYVGM"
     gene            complement(2065..2616)
                     /locus_tag="VM406_14630"
     CDS             complement(2065..2616)
                     /locus_tag="VM406_14630"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HVL77248.1"
                     /translation="MDHLNEERIASELAYDGAFLKVNKDKVRLPDGKQSEREYICHPG
                     AVVVLPLFDDGSVLLERQFRYPLQAVFIEFPAGKIDPGEDHLESAKRELLEETGYTAR
                     DWHYLATIHNAIAYSDEHLEIFLARGLTAGERKLDEGEFLETFTATVPEMLKWVREGK
                     ITDVKTVIGTFWLERILDGRWQP"
     gene            complement(2668..2973)
                     /locus_tag="VM406_14635"
     CDS             complement(2668..2973)
                     /locus_tag="VM406_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HVL77249.1"
                     /translation="MDVSVSAWLVILLAVGAANIPFFNERLFALIALPLALKPVWLRL
                     VELLILYFVVLAIAWALEERIGNVFRQGWEFYVITACLFLVFAFPGFVWRYLRRRRD"
     gene            complement(2983..4470)
                     /gene="nuoN"
                     /locus_tag="VM406_14640"
     CDS             complement(2983..4470)
                     /gene="nuoN"
                     /locus_tag="VM406_14640"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HVL77250.1"
                     /translation="MENLNLALALPEIFLAIAICAILLIDAFSKSGARDLTFVLSLGA
                     IVVAGLLAYADFGNPTAYAFSNMYVSDSMASLLKVFSCVAIALTLIYSRGYAGDRGMI
                     GGNTLGGEFYLLALFSLLGQFVMISGSNFLSIYLGLELMSLSLYALVALRRGHSVSTE
                     AAMKYFVLGALASGFLLYGISMLYGATGSLDLQEVAAAVASGNVDRTVLVFGLVFLVA
                     GLAFKLGAVPFHMWVPDVYQGAPTAVTLLLGGAPKLATFAIIIRLLVEGLLPLAFDWQ
                     QMLTVLAVLSIAIGNFTAIVQVNIKRMLAYSSIAQMGFMLLGLLSGVSNGDASQAGNA
                     YSSALFYAITYVITTVGAFGVIMLLARAGFEAESIDDFKGLNQRSPWFAFVMLALMFS
                     LTGIPPMVGFYAKLSVLQAVVANGQVWLAVFAVLMSVVGAFYYLRVVKVMYFDDPVDQ
                     VQLAGSHDMRVMLSLNGAAVVLLGVLPGPLMALCAYAVMQSLGGV"
     gene            complement(4515..6002)
                     /locus_tag="VM406_14645"
     CDS             complement(4515..6002)
                     /locus_tag="VM406_14645"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HVL77251.1"
                     /translation="MMQSTFPYLSLAIWSPIVFGLIVLAIGRDENPTPTRVLSLIGAI
                     VSFLVTLPIYLQFDNTASAMQFVEQAMWIDRFNIQYLLGVDGISMWFVLLTAFITVIV
                     VIAGWEVIEKRVAQYMGAFLILSGAMIGVFTALDGLLFYVFFEATLIPMFIIIGVWGG
                     PNRVYAALKFFLYTLLGSLLTLVAVIYLYMESGSFQILDWHTLPLTMAAQVYIFLAFL
                     MAFAVKVPMWPVHTWLPDAHVEAPTGGSVVLAAIMLKLGAYGFLRFSLPIAPDASHAL
                     SGLMISLSLIAVIYIGLVALVQADMKKLVAYSSIAHMGFVTLGFFIFNDMGVQGGIVQ
                     MISHGFVSGAMFLCIGVLYDRVHSREIADYGGVINTMPRFAAFFVLFSLANAGLPGTS
                     GFIGEFMVILASVQFNFWIGVLAATALILGAAYSLWLVKRVVFGAIAHQHVADLQDLN
                     RREFFMLGVLAIAVILMGVYPAPFTDVMQVSVSNLLDHVSVSKLK"
     gene            complement(6029..>7493)
                     /gene="nuoL"
                     /locus_tag="VM406_14650"
     CDS             complement(6029..>7493)
                     /gene="nuoL"
                     /locus_tag="VM406_14650"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HVL77252.1"
                     /translation="IGLLVAYTGSLNYNEVFERREELALLTLPGTDWLLITVACICLF
                     IGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGIFMVARMSPLFELSETALS
                     VVLVIGAITALFMGFLGIIQNDIKRVVAYSTLSQLGYMTVALGASAYSIAVFHLMTHA
                     FFKALLFLGAGAVIIGMHHDQDIRNMGGLRKYMPITWITSLLGSLALIGTPLFSGFYS
                     KDSIIEAVHLVNIPGAAFAEFAVMAGVFVTAFYSFRMYFLVFHGEERWGKAHAQHQDH
                     SHDEHSDEAHHGLAPGEKPHEAPLVVTLPLILLAIPSVIIGYLTIGPMVHGDFFEGVI
                     FVSDAHPAMATLTEHFDGAWAMTLHAFTTMPFWLALAGVAAAYYCYMVNPRVPAWFFD
                     RFRAVHTLLDNKYYMDKINDVVFAGGARALGAGLWKGGDRAFIDGVLVDGSARLVGWF
                     STVVRRLQSGYIYHYAFVMIIGVLLFLLVFLPSPFAK"
BASE COUNT         1679 a         2283 c         2298 g         1233 t
ORIGIN      
        1 ccgccagcac gcttgcaaaa cgcgcgccca gtccgctcga tgcacccgtg accagcgcgg
       61 tctttccctg caaattcagc tctgtactca tcggttctcc tccggtttat gtgttcaatt
      121 caaatctcgt tgtcgccgca cgttatcatt acgggttcga tatggaacta gaagcggttg
      181 cctagtaatc ccggccgaaa tgcacgacaa ttagcacgtt cgttcgtttt tcgataataa
      241 cacttacatt taggtgactc ccgatgatcc tgacacaaga tccccgcttc ctcgaacagt
      301 atggtccacg cgagtcgatg gaatacgatg tcgtgatcgt cggcggcggg ccggcgggcc
      361 ttgcgacggc gatccggctc aagcagatca atagcgatat ttcggtggtg gtgctcgaga
      421 agggctccga gcccggcgcc cacatcctgt ccggcgcggt catggatccg cgcgcgatga
      481 atgaactgtt cccgaattgg aaggaactgg gcgcgccgtt gaaccagccc gtcaccggcg
      541 acgatctcct gacgctgtcc gaaacgggcg tgcgccgcgt gccggactgg ctgatgccgc
      601 gcaatttcca taacgacggc tgctacgtga tctcgctgtc gaacgtggtg aagtggatgg
      661 cgcagcaggc ggaaggtctc ggcgtggaaa tcttccccgg attttccgca gccgaagtgc
      721 tctacgacga gcagggccgc gtcaagggcg tggccaccgg caacatgggc atcgagaaaa
      781 acggcgagcc gggtccgaat ttccagctcg gcatggaact gctcggcaag tacaccatct
      841 ttgcggaagg tgcgcgggga catctcggca agcagttgat tgccaagttc aagctcgacg
      901 agggcaagga tccgcagtcg ttcgcgatcg gcatcaagga gctgtgggag atcgatccgg
      961 ccaaggcgaa gcccggcctc gtcgtacata cggccggctg gccgatcgaa gacgacacct
     1021 ttggcggcgg cttcctctac cacctggaag acaacaaggt cacgctcggc tacgtgatcg
     1081 ggctggacta ccagaacccg tacatgagcc cgttcgagga gatgcagcgc tggaagctgc
     1141 atccggagat ccgcaagcac atcgaaggcg gcaagcggct gggctacggc gcgcgagcga
     1201 tcaacaacgg cagtccgcag gcgctgccga agacagtgtt cgccggcggc gccctggtcg
     1261 gttgcgacgc gggctacatg aacgctgcac gcatcaaggg cagccacgcg gcgatcaagt
     1321 ccggcatgct gtgcgcggaa gcactggccg acgccctcgc cgccggccgc tcgctggacg
     1381 agctgagcgc ctatccggaa gcgttcgaga agagctggct gaacgaggaa ctccagcaga
     1441 cccgcaactt caagctctgg ttcaagaagg gcaatacgat gggtcagctg atgaccggcg
     1501 tcgagcagtg gttcctgccg aagatcggca ttgcgagccc gccctggacc ctgcatcgcg
     1561 acaagccgga ccacgtctac ctcaagccgg ccgaccagtg ccgcccgatc gactatccta
     1621 aaccggatgg ccagatcacg ttcgaccgcc tgtcgtcggt cttcgtcagc aacaccaacc
     1681 acgaagagaa tcagccatcg cacctcacgc tgaaggatga aggcattccg gtcgaggtga
     1741 atctgaacaa gtatgcgggg ccggaaagcc gctactgccc ggccggcgtc tacgagtttg
     1801 tcaaggctga cgatggccgg gagcgcctgc agatcaatgc gcaaaactgc gtgcattgca
     1861 agacctgcga catcaaggac ccgacccaga acatcgtctg ggtgacgccg gaaggcggcg
     1921 gtgggccgaa ctacgtaggc atgtaagggc aagccagatg aaaacgccgc tcgatgagcg
     1981 gcgtgcttgc gaagtcttgt tgtcgatccg gcgagcgccg ggctccccgt gcagcgtgcg
     2041 tcgcgcccgc tggtcgactt ccggttatgg ctgccagcgg ccgtcgagaa ttctctctag
     2101 ccagaaagtt ccgatcaccg tcttcacatc ggtgatcttc ccttccctca cccacttcaa
     2161 catctccggc accgttgcgg tgaatgtttc gagaaactcg ccctcgtcga gcttgcgctc
     2221 gccggccgtc aggccccgtg cgaggaagat ttccagatgc tcgtccgaat aggcgattgc
     2281 gttgtggatg gtggcgagat agtgccagtc gcgcgccgta tagccggtct cttccagcag
     2341 ttcgcgtttt gcggattcca gatgatcctc gccgggatcg atcttcccgg ccgggaattc
     2401 gatgaacaca gcctgtagcg gatagcggaa ctggcgctcg agcagcacgc tgccgtcatc
     2461 gaacagcggc agcacgacca ccgctccggg atggcagatg tactcgcgct cggactgttt
     2521 gccgtccggc aggcgtacct tgtctttgtt taccttgagg aacgcaccgt cataggcgag
     2581 ttcggaggcg atgcgttctt cgttcagatg atccatcgtg gctccaaaac attggacact
     2641 ggcgccgcgc ttgtatgccg aggccgttca gtcgcggcgt cgacgcaggt agcgccagac
     2701 gaatccggga aacgcaaaca caaggaacag gcaggccgtg atcacgtaaa actcccagcc
     2761 ctgcctgaag acattgccga ttcgctcctc gagcgcccag gcaattgcca atacgacgaa
     2821 gtagaggatc agcagttcca ccagccgcag ccagaccggc tttagcgcaa gcggcagcgc
     2881 aatgagcgca aacaggcgct cgttgaagaa cgggatgttg gcggcgccca ccgccaacag
     2941 tatgaccagc caggccgaga ctgaaacatc caccggccgg ccttacacgc caccgagcga
     3001 ctgcatgacc gcgtacgcgc acagcgccat caacgggccg ggcagtacgc caagcagcac
     3061 cacggcggcg ccgttcaggc tgagcatgac gcgcatgtca tgagaacctg caagctgaac
     3121 ctgatcgacc ggatcgtcga agtacatgac cttgacgacg cgcaggtagt agaacgcgcc
     3181 aaccaccgac atcaggaccg cgaacacggc gagccagacc tggccgttcg cgacgaccgc
     3241 ctgcagcacc gacagcttcg catagaagcc gaccataggc ggaattccgg tcagcgagaa
     3301 catcagcgcc agcatcacga aggcgaacca ggggctgcgc tggttcagcc ccttgaaatc
     3361 gtcgatgctc tcggcttcga agccggcgcg agcgagcagc atgatcacgc caaaggcgcc
     3421 gaccgtggtg atcacatagg tgatcgcata gaacagcgcc gagctgtaag cgttgcccgc
     3481 ctgcgacgca tcgccgttgg atacgcccga cagcaggcca agcagcatga agcccatctg
     3541 cgcgattgac gaatacgcga gcatgcgctt gatgttgact tgcacgatcg cggtgaagtt
     3601 gccgatcgcg atcgacagca ccgcgagtac agtcagcatc tgctgccagt cgaaggccaa
     3661 gggcagcagg ccttccacga gcaggcgaat gatgatggca aacgtggcca gcttcggtgc
     3721 gccacccagc aggagcgtca ctgccgtcgg cgcgccctgg tacacatccg ggacccacat
     3781 gtggaacgga accgcaccca gcttgaaagc caggccggcg accaggaata ccaggccgaa
     3841 caccagcacg gtgcgatcga cgttgccgga agcaacagcg gccgccactt cctgcagatc
     3901 cagcgagccg gtggcgccat acagcatcga gatgccatag agcaggaagc cggaggccag
     3961 tgctccgagc acgaagtact tcatcgcggc ctcggtcgag accgaatggc cgcgccgcaa
     4021 agctaccagc gcgtagagcg acagcgacat cagctcgagg ccaaggtaga ttgacaggaa
     4081 attgctgccg gagatcatca cgaactggcc gagcagcgag aacagcgcga gcaggtagaa
     4141 ctcgccgccg agcgtgttgc ctccgatcat gccccgatcg cccgcatatc cgcgcgagta
     4201 gatcagtgtc aacgcaattg ccacgcacga gaacaccttg agcagactcg ccatggaatc
     4261 ggacacgtac atgttcgaga acgcgtaggc ggtcggattg ccgaagtccg cataagcaag
     4321 caggccggcc acgacgatcg cgccgagcga cagcacaaag gtaaggtcgc gtgcgccgct
     4381 cttcgagaat gcatcgatca gcagaattgc gcaaatcgca atcgcgagaa agatctcggg
     4441 gagggccaat gccaggttca ggttttccat cttttcatcg tcgctatgtt gcgctcgctg
     4501 gttcgtttgc cgtgttactt gagtttggac acggagacat ggtcgagcag attcgagacc
     4561 gagacctgca tgacgtcggt gaacggcgcc ggatacacgc ccatcaggat cacggcgatc
     4621 gccagcacgc ccagcatgaa gaactcgcgc cggttcaggt cctgcaggtc ggctacgtgc
     4681 tgatgggcga tcgcaccgaa cacgacccgc ttgaccagcc acagcgagta cgctgcaccg
     4741 aggatcagcg cggtggcggc gagcacgcca atccagaagt tgaactgcac ggaagccagg
     4801 atcaccatga attcaccgat gaagcccgag gtgccgggca gtcccgcatt ggccagcgag
     4861 aacagcacga agaacgcggc gaagcgcggc atggtattga tcacgccacc gtagtcggcg
     4921 atctcgcggg aatgcacgcg gtcgtacagc acgccgatac acaggaacat cgcaccggac
     4981 acgaagccgt gggagatcat ctgcacgatg ccgccctgca cgcccatgtc attgaaaatg
     5041 aagaatccga gcgtcacgaa acccatgtgg gcgatcgacg aatacgcgac cagctttttc
     5101 atgtcggcct gcaccagcgc gaccaggccg atgtagatca ctgcgatcag cgacagcgag
     5161 atcatcaggc cggacaacgc atggctggca tccggggcga tcggcagcga gaaacgcagg
     5221 aagccgtagg cgccgagctt cagcatgatc gccgctagca ctacagaacc gccggtcggc
     5281 gcctcgacgt gtgcgtctgg cagccaggtg tggaccggcc acatcggcac cttcaccgcg
     5341 aatgccatca ggaaggcaag gaagatgtag acctgggctg ccatcgtcaa cggcaaggta
     5401 tgccagtcga ggatctggaa gctgccggac tccatgtaca ggtaaatcac cgcaacgagg
     5461 gtcagcagcg aacccagcag cgtgtacagg aagaacttca gcgccgcata gacgcggttc
     5521 ggcccgcccc acacgccgat gatgatgaac atcgggatca gcgtggcttc gaagaacacg
     5581 tagaacagca ggccgtccag tgccgtgaac acgccgatca tcgcccccga caggatcagg
     5641 aacgcgccca tgtactgggc aacgcgcttc tcgatcactt cccatccggc gatcacgacg
     5701 atgaccgtga tgaaggcggt aagcagcacg aaccacatcg agatgccgtc cacgccgagc
     5761 agatactgga tgttgaagcg gtcgatccac atcgcctgct cgacgaactg catggccgaa
     5821 gccgtgttgt cgaactggag gtagatcggc agcgtaacca ggaagctgac gatggcgccg
     5881 atcagcgaca agacgcgtgt cggggtcgga ttctcgtcgc gcccgattgc caggacgatc
     5941 agaccgaata cgatcgggct ccagatcgca aggctcaggt aaggaaaagt tgactgcatc
     6001 atggttatta ttcgcttact tcgccaactt atttggcaaa cggagacggc aggaagacga
     6061 gcaggaaaag cagcacgccg atgatcatca cgaaagcgta gtgatagatg tagccggact
     6121 gcagacggcg tacgacagtg gagaaccagc cgaccagcct ggcgctgccg tcgaccagca
     6181 ccccgtcgat gaatgcccgg tcaccaccct tccacagtcc tgcaccgagg gcacgcgcgc
     6241 cgccggcgaa gactacgtcg ttgatcttgt ccatgtagta cttgttgtcg agcagggtat
     6301 gcaccgcgcg gaaccggtca aagaaccacg cgggaacgcg cggattgacc atgtagcagt
     6361 agtaggctgc ggccacacct gccagcgcca gccagaacgg catggtggtg aatgcatgca
     6421 gcgtcatcgc ccaggcaccg tcgaagtgtt cggtcagcgt agccatcgcc ggatgggcat
     6481 cgctcacgaa gatcacacct tcgaagaagt cgccgtgcac catcgggccg atcgtcaggt
     6541 agccgatgat gaccgacggg atcgccagca ggatcagcgg cagcgtgacc accagtggag
     6601 cttcatgcgg cttctcgccc ggggccaggc cgtgatgggc ttcatcgctg tgttcgtcgt
     6661 ggctatggtc ctggtgctgg gcatgcgcct tgccccaacg ctcttcgcca tggaagacga
     6721 ggaagtacat gcggaacgag tagaacgcgg tcacgaacac gcccgccatg actgcgaact
     6781 ccgcgaacgc cgcgcccggg atgttcacca gatggaccgc ctcgatgatg ctgtccttcg
     6841 aatagaaccc ggagaacaga ggcgtgccga tcagcgcgag cgagccgagc aaagacgtga
     6901 tccaggtgat cggcatgtac ttgcgcagtc cgcccatgtt gcggatgtcc tggtcgtgat
     6961 gcatgccgat gatgaccgcg ccggccccca ggaaaagcag cgccttgaag aacgcgtgcg
     7021 tcatcagatg gaacacggcg atcgagtagg cggaggcgcc cagtgcgacc gtcatgtatc
     7081 cgagctggga cagcgtcgag tatgcaacca cacgcttgat gtcgttctgg atgatgccga
     7141 ggaagcccat gaacagcgcc gtaattgcgc cgatgaccag cacgacggac agcgcggttt
     7201 cagacagctc gaacagcggc gacatgcgcg cgaccatgaa gatgccagcc gtcaccatag
     7261 tcgccgcgtg gatcagcgcc gagatcgggg tcggcccttc catcgaatcc ggaagccaga
     7321 cgtgcaacgg gaactgtgcg gacttgccca ttgcgccgat gaacaggcag atgcaggcaa
     7381 cggtgatcag cagccaatcg gtacccggca gcgtcagcag ggccagctct tcgcggcgct
     7441 cgaacacctc gttgtagttc agcgaaccgg tgtaggcgac cagcaggcca atg
//
LOCUS       DATLGF010000472         2375 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_13634,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000472 DATLGF010000000
VERSION     DATLGF010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13634"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VM406_14655"
     CDS             <1..410
                     /locus_tag="VM406_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HVL77253.1"
                     /translation="DDRIAQPDARQVSDAVIAIRSRKLPDPAVIGNAGSFFKNPLVPA
                     QQRDALLQRYPQLVSYPQADGSFKLAAGWLIDQCGWKGRDLGRAGVYEKQALVLVNRG
                     GASGREIAELAAAVQADVFEKFGVRLEPEPVFV"
     gene            complement(462..782)
                     /locus_tag="VM406_14660"
     CDS             complement(462..782)
                     /locus_tag="VM406_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HVL77254.1"
                     /translation="MSTQFDNVSVVKKANIYFDGKCVSHTVQFADGTKKTIGVIFPSA
                     LTFNTGAPEVMELNAGKCRVRLKGEDAWTSYEGGQRFNVPGNSSFDIEVIETVDYVCH
                     FEGA"
     gene            complement(794..2134)
                     /gene="argG"
                     /locus_tag="VM406_14665"
     CDS             complement(794..2134)
                     /gene="argG"
                     /locus_tag="VM406_14665"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HVL77255.1"
                     /translation="MSTILQSVPVNQKVGIAFSGGLDTSAALLWMRQKGAIPYAYTAN
                     LGQPDEPDYDAIPKKARQYGAEQARLIDCREQLVAEGIAALQSGAFHITTAGATYFNT
                     TPLGRAVTGTMLVAAMQEDQVDIWGDGSTFKGNDIERFYRYGLLVNPALRIYKPWLDD
                     TFIHELGGRKEMSEFMIKSGFDYKMSVEKAYSTDSNMLGATHEAKDLENLNTGINIVQ
                     PIMGVAFWRDDVEIKREQVTVRFEEGRPVALNGMSFANAVDLMMEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGLALLFIAYERLVTGIHNEDTIEQYRENGRKLGRLLYQG
                     RWFDPQAIMLREASQRWVARAITGEVTIELRRGNDYSILNTESPNLTYAPERLSMEKV
                     EDAPFTPQDRIGQLTMRNLDIADTREKLGIYSKAGLLSSGSSVALPRLHKKSGE"
     gene            complement(2201..>2375)
                     /locus_tag="VM406_14670"
     CDS             complement(2201..>2375)
                     /locus_tag="VM406_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HVL77256.1"
                     /translation="QRARPDALFMHCLPAHRGEEVAADVIDGPQSVGWDAAENRLHVQ
                     KALLEYLLLGRVK"
BASE COUNT          372 a          728 c          796 g          479 t
ORIGIN      
        1 gcgatgaccg catcgcccaa ccggatgcgc gccaggtgag cgatgcggtc atcgcgattc
       61 gcagccgcaa gctgccggac ccggccgtga tcggcaacgc cggcagcttt ttcaagaacc
      121 cgctcgttcc cgcgcagcag cgcgatgcat tgctgcagcg ttatccgcag ctggtcagct
      181 atccgcaagc cgacggcagt ttcaagctgg cggcgggatg gctgatcgat cagtgcggct
      241 ggaagggcag ggacctcggg cgcgccggcg tgtacgagaa gcaggcgctg gtgctggtca
      301 accgcggcgg agcaagcgga cgggagatcg cggaactcgc cgctgcggtg caggcggatg
      361 tgttcgagaa gtttggcgtg cgattggagc cggagccggt gttcgtctga gcggcggcgc
      421 aaaagccggg caaaaccgcc gcccggctcc ggcgagcggg tttacgcccc ttcgaaatgg
      481 cagacgtagt ccaccgtctc gatcacttcg atatcaaagc tcgaatttcc cggcacattg
      541 aagcgctggc cgccttcgta gctcgtccac gcgtcctccc ccttcaggcg cacgcggcac
      601 ttgccggcgt tgagttccat cacttccggt gcgccggtgt tgaaggtcag cgcggacggg
      661 aagatcacgc ctatggtttt cttcgtgccg tcggcgaact gcaccgtgtg cgagacgcac
      721 ttgccgtcga agtagatgtt ggctttcttg acgacggata cgttgtcgaa ttgcgtgctc
      781 atcaacggtc cccttattcg ccgctcttct tgtgcaggcg cggcagcgcc accgaggaac
      841 cggaggagag caggccggcc ttgctgtaga tgccgagttt ttcgcgggta tcggcgatgt
      901 ccaggttgcg catggtcagc tggccgatgc gatcctgcgg cgtgaacggc gcgtcttcca
      961 ccttttccat cgacagacgc tccggcgcat aggtcaggtt cggcgactcg gtgttcagga
     1021 tcgagtagtc gttgcctcgg cgcagttcga tcgtcacctc gccggtgatc gcacgcgcga
     1081 cccagcgctg cgacgcctcg cgcagcatga tcgcctgcgg atcgaaccag cggccttggt
     1141 acagcaggcg cccgagcttg cggccgttct cgcggtactg ctcgatcgtg tcttcgttgt
     1201 gaatgccggt caccaggcgc tcgtaggcaa tgaacagcag cgccagtccg ggagcctcgt
     1261 agatgccgcg gctcttcgct tcgatgatgc ggttttcgat ctggtcgctc atacccagcc
     1321 cgtggcggcc gccgatgcgg ttggcctcca tcatcagatc caccgcgttg gcgaagctca
     1381 tgccgttcag cgccaccgga cgcccttcct cgaagcgcac cgtcacctgt tcgcgcttga
     1441 tctcgacgtc gtcgcgccag aacgccacgc ccatgatggg ctggacgatg ttgatcccgg
     1501 tattgaggtt ctccaggtcc ttcgcctcgt gggtggcgcc cagcatgttg gaatcggtgg
     1561 agtaggcctt ctcgaccgac atcttgtagt cgaagccgga cttgatcatg aactccgaca
     1621 tttccttgcg gccgcccagt tcatggatga aggtgtcgtc cagccagggc ttgtagatgc
     1681 gcagcgccgg gttcaccagc aggccatagc ggtagaagcg ctcgatgtca ttgcccttga
     1741 aggtcgaacc gtcgccccag atgtcgacct ggtcttcctg catcgcggca accagcatcg
     1801 ttccggtgac tgcgcggccc agcggcgtgg tgttgaagta ggtggcgccg gcggtggtga
     1861 tgtggaaggc gccgctctgc agcgcggcaa tcccttcggc gaccagctgc tcgcggcagt
     1921 cgatcaggcg cgcctgttcg gcgccgtact gcctggcctt cttcggaatc gcgtcgtaat
     1981 cgggctcatc cggctggccg aggttggccg tgtacgcgta ggggatggct cccttctggc
     2041 gcatccacag cagcgcggcg ctggtgtcga ggccgccgga gaaggcgatg cccactttct
     2101 ggttgacggg aacggactgg agaatggtcg acatggtttc tgcttcgctg tgatgggggt
     2161 ggaacgatgg gtgattggac gatggcggct cgacgggccc ttatttgacc ctgcccagca
     2221 gcaggtattc aagcagcgcc ttctgcacgt gcaggcggtt ctcggctgcg tcccagccga
     2281 cggattgcgg gccgtcgatg acgtcggcgg cgacttcctc gccgcggtgg gcgggcaggc
     2341 agtgcatgaa cagtgcatcg gggcgggcgc gttgc
//
LOCUS       DATLGF010000473         3617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_122787, whole genome shotgun sequence.
ACCESSION   DATLGF010000473 DATLGF010000000
VERSION     DATLGF010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122787"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VM406_14675"
     CDS             complement(<1..413)
                     /locus_tag="VM406_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006713154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HVL77257.1"
                     /translation="MQRSKMMDYDQPLVWVVLLLMLLGMVMVYSASIALPDSPKYANY
                     SNSHFLMRQATFVGLALIAGMLAFRVPVQTWQRLAPWLFVGAIVLLVLVLVPGIGKGV
                     NGARRWISLKFFNIQPSELMKLFVVLYAADYTVRK"
     gene            complement(470..1972)
                     /gene="murD"
                     /locus_tag="VM406_14680"
     CDS             complement(470..1972)
                     /gene="murD"
                     /locus_tag="VM406_14680"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HVL77258.1"
                     /translation="MDYQGKHALVLGLGESGLAIARWLARGGARLRVADTRDEPQRLP
                     ELREFAPEAEFIGGAFEPALLDGVDFVAVSPGLAPTRELAAIVPAAAERGIPILSEIA
                     LFAQALAALREARGYAPKLIAITGTNGKTTVTSLTGLLCRRAGLRTEVAGNISPAALD
                     CLRTALDADTLPEVWVLELSSFQLHWAADAFEADVATVLNVTQDHLDWHGDMDAYAAD
                     KARIFGARTIRLLNRDDARVMQMAAPLAANISFGADEPEQAGSFGLVEDNGMQWLAVA
                     EADEDDDGAPKRRRKKDNVDQPVSIKRLMPVDALQLRGRHNALNALAALALCRAIGLA
                     LAPLLHGLREYRGEPHRVELVANIGGVDYVDDSKGTNVGATVAALNGLGAGQPEPRLI
                     LIAGGDGKGQDFTPLVEPIARHVRAVVLIGKDAAAIRAALDGTVVQMIDCQTLEQAVA
                     KAAELARPADIVLLSPACASLDMFRNYGHRAEVFVAAVRELALSRGEVLA"
     gene            complement(1972..3141)
                     /gene="mraY"
                     /locus_tag="VM406_14685"
     CDS             complement(1972..3141)
                     /gene="mraY"
                     /locus_tag="VM406_14685"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HVL77259.1"
                     /translation="MLLWLAQYFQEEIGPLRVFSFITFRAVFATITALIIGLVAGPAV
                     IRMLTRLKVGQAVRTYGPQSHMVKNGTPTMGGALVLLAIGISTVLWSDWSNRFVWVVM
                     LVTLGFGAIGWVDDYRKVVYKNPEGMSSREKFFWQSLIGLIAAVYLAFSVSAPTNTEV
                     LDLFLDWVRSGFNIDLPPKADLIVPFFKSISYPLGVWGFIALTYFVIVGTSNAVNLTD
                     GLDGLAIMPTVMVGSALGLIAYLTGSAVYSKYLFIPHIPGSGELLIFCGAMAGAGLAF
                     LWFNAYPAQVFMGDVGALALGGALGTIAVIVRQEIVLFIMGGIFVVETLSVMLQVTWF
                     KYTKRRYGVGRRILLMAPLHHHYEQKGWAETKVVIRFWIITMMLVLFGLSTLKLR"
     gene            complement(3143..>3617)
                     /locus_tag="VM406_14690"
     CDS             complement(3143..>3617)
                     /locus_tag="VM406_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HVL77260.1"
                     /translation="DTIARGLEAFMPVSGRLQRKTAASGATVIDDTYNANPDSVRAAI
                     DVLAGFGAPRVLVLGDMGEVGSEGKSFHEEIGAYAAERGIEHVLVTGDLMRHAAAAGA
                     PVARHFDDFDSLKRDAEALATAGATVLVKGSRFMRMERVVNHLVDPQSAKQTETH"
BASE COUNT          667 a         1218 c         1171 g          561 t
ORIGIN      
        1 tgcttgcgca ccgtgtaatc ggccgcgtac agcacgacaa acagcttcat cagctcggaa
       61 ggctggatgt tgaagaactt cagcgagatc cagcgacgcg cgccgttgac gcccttgccg
      121 atgccgggca cgagcacgag caccagcagc acaatcgcgc cgacgaacag ccagggcgcc
      181 agacgctgcc aggtctgaac gggcacgcgg aaggcgagca tgccggcaat cagcgccagg
      241 ccgacgaagg tcgcctgacg catcaggaag tgcgagttcg aatagttcgc atacttcggc
      301 gaatccggca gcgcgatcga ggccgaatac accatcacca tccccagcag catcaggagc
      361 aggaccaccc acaccagcgg ctggtcgtag tccatcatct tcgagcgctg catgacgccg
      421 ggcagtggcg cctgctttgc ggaagacgag aaggatggga acgcgaatct cacgcgagca
      481 cctcgccgcg tgaaagcgcg agctcgcgca ccgctgcaac gaacacttcg gcgcgatggc
      541 cgtaattgcg gaacatgtcc aggctcgcgc acgccggcga cagcagcacg atgtccgccg
      601 ggcgcgcgag ctcggccgct ttcgcgaccg cctgctccag cgtctggcaa tcgatcatct
      661 gaacgacggt gccgtcgagc gccgcgcgga tcgcggcggc gtccttgccg atcagcacga
      721 cggcccgcac gtggcgcgca atgggctcga ccagcggcgt gaagtcctgg cccttgccat
      781 caccgccggc gatcaggatc agtcgcggct ccggctgtcc ggcgccaagg ccgttcaatg
      841 ccgccaccgt cgcaccaaca ttggtgccct tgctgtcatc gacatagtcg acgccgccga
      901 tgtttgccac cagctcgacc cggtgcggct cgccgcggta ctcgcgcagg ccgtgcagca
      961 gcggcgcgag cgccaggccg atcgcccggc acaaggccag cgcggccagc gcattgagcg
     1021 cattgtggcg cccgcgcagc tgcagcgcgt cgaccggcat caggcgcttg atcgataccg
     1081 gctggtcgac gttgtccttc ttgcggcggc gcttcggcgc gccgtcgtcg tcttcgtccg
     1141 cctcggccac ggccaaccat tgcatgccgt tgtcctcgac caggccgaag ctgccggcct
     1201 gctccggctc gtcggcgccg aagctgatgt tcgctgcaag aggcgcggcc atctgcatca
     1261 cgcgcgcatc gtcgcggttg agcaggcgta tcgtccgtgc gccgaagatg cgcgccttgt
     1321 cggccgcgta ggcgtccatg tcgccatgcc agtccaggtg gtcctgcgtg acgttgagga
     1381 cggtcgcgac gtcggcctcg aacgcgtcgg ccgcccagtg cagctggaag cttgacagtt
     1441 caagcaccca gacctcgggc agcgtgtcgg cgtcgagcgc cgtgcgcagg cagtcgagcg
     1501 cggccgggct gatgttgccc gcaacttcgg tgcgcagccc ggcacgccgg cacaagagcc
     1561 cggtcaggct ggtgacggtg gtcttgccgt ttgtgccggt gatcgcgatc agcttcggtg
     1621 catacccgcg cgcctcgcgc aacgctgcca gtgcctgcgc aaacagtgcg atctcggaca
     1681 ggatgggaat gccgcgctcg gccgcggccg ggacgatggc ggccagctcg cgcgtcggcg
     1741 caaggccggg gctgaccgcg acgaaatcta cgccgtcgag cagcgcgggc tcgaacgcgc
     1801 cgccgatgaa ctcggcctcg ggcgcaaact cgcgcagctc cggcaggcgc tgcggctcgt
     1861 cacgcgtatc ggccacgcgc aggcgcgcgc cgccgcgggc gagccagcgg gcgatcgcca
     1921 ggcccgattc accgagcccg aggacgagag cgtgttttcc ctggtagtcc atcagcgcag
     1981 cttcaaggtg gacagcccga acagtacgag catcatcgtg atgatccaga agcggatcac
     2041 gaccttcgtt tcagcccatc ccttttgttc atagtgatgg tgcagcggcg ccatcagcag
     2101 gatgcgccgg ccgacgccgt agcgccgctt cgtgtacttg aaccaggtca cctgcagcat
     2161 caccgagagc gtctcgacga caaagatgcc gcccatgatg aagagcacga tctcctggcg
     2221 cacgatcacc gcaatggtgc cgagcgcgcc gcccagcgcc agcgcgccga cgtcgcccat
     2281 gaagacctgc gccggatagg cgttgaacca caggaaggcc agtcccgcac ccgccatcgc
     2341 cccgcagaag atcagcagtt cgccggaacc cgggatgtgg ggaatgaaca ggtacttcga
     2401 atacaccgcg ctgccagtga gataggcgat caggccgagc gccgagccga ccatcacggt
     2461 cggcatgatg gcaagcccgt ccaaaccgtc ggtcaggttc accgcattgc tggtaccgac
     2521 gatgacgaaa taggtcagcg cgatgaagcc ccagacgccg agtggatagc tgatcgattt
     2581 gaagaacggg acgatcaggt cggctttagg cggcaggtcg atgttgaagc cggaacgcac
     2641 ccagtccagg aacaagtcca gcacttcggt attggtcggc gccgacaccg agaacgcgag
     2701 atagacggcc gcaatcagcc cgatcagcga ctgccagaag aacttctcgc gcgaagacat
     2761 gccctccggg ttcttgtaga cgaccttgcg gtagtcatcg acccagccga tcgcgccgaa
     2821 accgagggta acgagcatca cgacccagac gaagcggttg ctccagtccg accacagcac
     2881 cgtagagatg ccgatcgcga gcagcaccag cgcgccgccc atcgtgggcg tgccgttctt
     2941 gaccatgtgg ctctgcgggc cataggtacg caccgcctgg ccgaccttca agcgggtcag
     3001 catgcggatc acggccgggc ctgcgaccag gccgatgatg agcgcggtga tcgtcgcgaa
     3061 caccgcacgg aaggtgatga aactgaacac gcgcagcggt ccgatctctt cctgaaaata
     3121 ttgtgcgagc caaagcagca tgtcagtgtg tttccgtttg tttggcggat tgcgggtcga
     3181 ccaggtggtt taccacccgc tccatccgca tgaagcgcga gcccttgacg agcacggtcg
     3241 cgccggccgt cgcgagggct tccgcatcgc gcttgagtga atcgaaatcg tcgaagtgac
     3301 gcgcgaccgg cgcgccggcc gcagccgcat gacgcatcag atcgccggtc acgagcacgt
     3361 gctcgatgcc gcgttctgcc gcgtaagcgc cgatctcttc gtggaagctc ttcccttcgc
     3421 tgcccacctc gcccatgtcg ccgagcacca gcacgcgcgg cgctccgaag ccggcgagca
     3481 cgtcgatggc ggcgcgtacc gaatcgggat tggcgttgta ggtatcgtcg atcacagtcg
     3541 cgccgctggc cgcggtcttg cgctgcaggc gcccgctcac cggcataaac gcctccaggc
     3601 cgcgcgcgat cgtatcg
//
LOCUS       DATLGF010000474         3570 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_217454, whole genome shotgun sequence.
ACCESSION   DATLGF010000474 DATLGF010000000
VERSION     DATLGF010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217454"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VM406_14695"
     CDS             <1..301
                     /locus_tag="VM406_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HVL77261.1"
                     /translation="LTLLASVGSTAPFVGLFGTVWGIYHALVAVSAAGTIQIDKVAGP
                     VGEALIMTAAGLVVAIPAVLGYNAFNRVNRVTLAELDAFAHDLHAYLTTGARVAR"
     gene            318..743
                     /locus_tag="VM406_14700"
     CDS             318..743
                     /locus_tag="VM406_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HVL77262.1"
                     /translation="MAFGGFSDGKQPAPMADINVTPMVDVMLVLLVIFIIAAPLLTHS
                     VKLDLPQAQSAPAPENGDAVTLSIDAEGAVFWNEERVDRAELEARLGQAAQLRPPPEL
                     QLRADKSTRYEVIAQVMSAAQAQGMTRMGFVTDASAGER"
     gene            745..1074
                     /locus_tag="VM406_14705"
     CDS             745..1074
                     /locus_tag="VM406_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HVL77263.1"
                     /translation="MQVRLDGRQLLTWPAFHQVCRAAFGFPDFYGANMDAWIDCMSGL
                     RDDDGMTRFSLGPEETLEIEVVHGDALRRQAPAILEALEECVEEVNVRCAEAGEPPVL
                     ALRVIRD"
     gene            1308..>3570
                     /gene="leuS"
                     /locus_tag="VM406_14710"
     CDS             1308..>3570
                     /gene="leuS"
                     /locus_tag="VM406_14710"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HVL77264.1"
                     /translation="MQEKYIPADIEKAAQDHWNAIDAYKTVEHAKDRNGQDKKKFYAC
                     SMLPYPSGKLHMGHVRNYTINDMLARFLRMNGHNVLMPMGWDAFGLPAENAALKNGVP
                     PAKWTYDNIAYMKKQMQAMGLAIDWSREIATCDPSYYKWNQWLFLKMLDKGIAYRKTQ
                     VVNWDPVDQTVLANEQVIDGRGWRTGALVEKREIPGYYLKITDYAEELLDHVVNNLPG
                     WPERVRVMQENWIGKSEGVRFAFTHDIRDADGAPIQDGKMFVFTTRADTIMGVTFCAV
                     APEHPLATHAAASNPKLAAFIDECKRGGTTEAELALKEKEGMPAGLTVTHPLTGAQVD
                     VWVGNYVLMTYGDGAVMGVPAHDERDFAFAKKYGLPIKQVIAVEGETYSTEAWQEWYA
                     DKQRGVTVNSGKYDGLPYKAAVDAVAADLAAQGLGEKKTTWRLRDWGISRQRYWGTPI
                     PIIHCESCGPVPVPEKDLPVVLPQDCVPDGTGNPLNKREDFLHVDCPQCGKPARRETD
                     TMDTFVDSSWYFMRYCDPKNDEQMVDGGADYWMPMDQYIGGIEHAILHLLYARFWTKV
                     MRDLGLVKVDEPFTNLLTQGMVLNHIYSRRTPAGGIEYFWPQDVDNVYDESGRIVGAK
                     LKSDGSEVNYDGVGTMSKSKNNGVDPQELINQYGADTARLFVMFASPPEQTLEWNDAG
                     VEGAHRFLRRVWAFAHAQAERVKASAYLDFAQLPESHKALRREMHKILQQADHDFRRI
                     QYNTGCGGNRDRPA"
BASE COUNT          693 a         1147 c         1163 g          567 t
ORIGIN      
        1 actgacgctc ctggcttcgg tcggctcgac cgctcccttc gtcggcctgt tcggcaccgt
       61 ctggggcatc taccacgcgc tggtcgcggt ctccgccgcc ggcaccatcc agatcgacaa
      121 ggtggcgggc ccggtcggcg aagcgctgat catgacggcc gccggcctgg tggtggcgat
      181 tccggcggtg ctcggctaca acgcattcaa tcgcgtcaac cgcgtcaccc tcgccgagct
      241 ggacgccttc gcccacgacc tgcacgcgta cctgaccaca ggcgcgcgcg tcgcccgcta
      301 ggaggccagg agtcgcaatg gcattcggcg gattcagcga cggcaagcaa ccggccccga
      361 tggccgacat caacgtcacg cccatggtcg acgtgatgct ggtgctgctc gtgatcttca
      421 tcatcgccgc cccgctgctg acccattcgg tgaagctgga cctgccgcag gcgcagtcgg
      481 cgcccgcacc cgaaaacggc gatgcggtca cgctgtcgat cgatgccgag ggcgccgtct
      541 tctggaacga ggagcgcgtg gatcgcgccg aactcgaggc gcggttgggg caggcggcgc
      601 aactgcggcc gccgcccgag ttgcaactgc gcgccgacaa atccacccgc tacgaagtca
      661 tcgcccaggt gatgtccgcc gcccaggccc agggcatgac gcgcatgggc ttcgtgaccg
      721 atgcgagtgc gggagagcgc tgagatgcag gtcaggctag acggccggca actgctgacc
      781 tggcccgctt ttcaccaagt gtgccgcgcc gcattcggtt ttcccgattt ctacggtgcg
      841 aacatggacg cctggatcga ctgcatgagc ggcctgcgcg acgatgacgg catgacccgt
      901 ttctcgctcg gcccggagga aacgctggag atcgaagtcg tgcacggcga cgcgctgcgc
      961 cggcaggcac cggcgattct cgaggcgctc gaagagtgtg tagaggaagt caatgtgcgc
     1021 tgcgccgaag cgggcgagcc gccggtgctg gcgctgcgcg tgatccgaga ctgaaggtcc
     1081 ccgcggggcc ggacgctgtt cctgaatcgc tgaacgcgac ttcgtcattc ccgttcagac
     1141 gcagcacttg ctttccacgc atggaggtct gcaagcatcg gcgccgggtt cccgcctgcg
     1201 cgggaacgac gcccaggcgt attggatctc cccgttttcg cgccgcaaca gcgcaaatcg
     1261 aaccagtata atggctggtt tcattcaacc tgccacgccc cttcgtcatg caagaaaagt
     1321 acatcccagc cgacatcgaa aaagcggcgc aagaccactg gaacgcgatc gacgcctaca
     1381 agaccgtaga acacgcgaag gacaggaacg ggcaggacaa gaagaaattc tacgcatgct
     1441 cgatgctgcc ctacccctcg ggcaagctgc acatgggcca tgtgcgcaac tacacgatca
     1501 acgacatgct cgcgcgcttc ctgcgcatga acggccacaa cgtgctgatg ccgatgggct
     1561 gggatgcgtt cggcctgccg gccgagaacg cggcgctgaa aaacggcgtg ccgccggcga
     1621 agtggaccta cgacaacatc gcctacatga agaagcagat gcaggcgatg gggctggcga
     1681 tcgactggtc gcgcgagatt gcgacctgcg acccgagcta ttacaagtgg aaccagtggc
     1741 tgttcctgaa gatgctggat aagggcatcg cctaccgcaa gacccaggtg gtgaactggg
     1801 acccggtgga ccagaccgtg ctggccaacg agcaggtgat cgatggacgc ggctggcgta
     1861 ccggcgcgct ggtcgagaaa cgagagatcc ccggctacta cctgaagatc accgattacg
     1921 cggaagaact gctcgaccac gtggtcaaca atctgcccgg ctggcccgag cgcgtgcgcg
     1981 tgatgcagga aaactggatc ggcaagagcg agggtgtgcg ctttgccttc acgcatgaca
     2041 tccgcgacgc cgacggcgcg ccgatccagg acggcaagat gttcgtgttt accacgcgtg
     2101 ccgacaccat catgggcgtg accttctgcg cggtggcgcc cgaacatccg ctggctaccc
     2161 acgcggccgc ttcgaatccg aagctcgccg cgttcatcga cgaatgcaag cgcggcggca
     2221 ccaccgaagc ggaactagcg ctcaaggaaa aggaaggcat gccggccgga ctgacggtca
     2281 cgcatccgct gaccggcgcg caggtcgacg tctgggtcgg caactatgtg ctgatgacct
     2341 acggcgacgg cgcggtgatg ggcgtgccgg cacacgacga gcgcgacttc gcgttcgcga
     2401 aaaagtatgg cctgccgatc aagcaggtga tcgccgtcga gggcgaaacg tattcgaccg
     2461 aggcctggca ggagtggtac gccgacaagc agcgcggcgt caccgtcaat tccggcaaat
     2521 acgacggcct gccctacaag gcggcggtgg acgcggtcgc ggccgatctg gccgcgcagg
     2581 gcctgggtga gaagaagacc acctggcgcc tgcgcgactg gggcatttcg cgccagcgct
     2641 actggggtac gccgatcccg atcatccact gcgagtcctg cggccccgtc ccggtgcccg
     2701 agaaggatct cccggtcgtg ctgccgcagg actgcgtgcc ggacggcacc ggcaatccgc
     2761 tgaacaagcg cgaggatttc ctgcacgtcg attgcccgca gtgcggcaag ccggcgcggc
     2821 gcgagaccga tacgatggac accttcgtcg actcgtcctg gtacttcatg cgctattgcg
     2881 atccgaagaa cgacgagcag atggtggacg ggggcgccga ctactggatg ccgatggacc
     2941 agtacatcgg cggcatcgag cacgcgatcc tgcacctgct gtacgcgcgc ttctggacca
     3001 aggtgatgcg cgacctcggc ctggtaaaag tggatgaacc gttcaccaac ctgctcacac
     3061 agggcatggt gctcaaccac atctactcgc gccgcacgcc ggccggcggc atcgaatact
     3121 tctggccgca ggacgtggat aacgtctacg acgagtcggg ccgcatcgtc ggcgcgaagc
     3181 tcaagagcga tggctcggaa gtgaactacg acggcgtcgg cacgatgtcg aagtcgaaga
     3241 acaacggcgt cgatccgcag gaactgatca accagtacgg tgcggacacc gcgcgcctgt
     3301 tcgtgatgtt cgcctcgccg ccggaacaga cgctcgaatg gaacgatgcc ggcgtcgaag
     3361 gcgcgcaccg tttcctgcgc cgcgtctggg cgttcgcgca tgcgcaggcc gagcgggtga
     3421 aggcgtccgc ctacctcgac ttcgcccagc tgccggaatc gcacaaggcg ctgcgtcgcg
     3481 agatgcacaa gatcctgcag caggccgatc acgatttccg ccgcatccag tacaacactg
     3541 gatgcggcgg aaatcgtgat cggcctgctg
//
LOCUS       DATLGF010000475         2302 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_234904, whole genome shotgun sequence.
ACCESSION   DATLGF010000475 DATLGF010000000
VERSION     DATLGF010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234904"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VM406_14715"
     CDS             complement(<1..606)
                     /locus_tag="VM406_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HVL77265.1"
                     /translation="MTHPLFTHSQEQLRQIAEDVLRFAREKGASDAAVEISEGKGLAV
                     SVRKGKIETIEQNKDKGIGVTVHLGAPGHVRRGNASTSDFSSRSLRDTVSAAYDIARF
                     TAEDDCAGLPDEDTLEKAPRDLQLFHPWDVTAEEAVELAQRCEGAAFGVDRRIANSEG
                     AGVHAQHSQFVYANSRGFIGGYPYSRHTLSVAPIARSGVDMQ"
     gene            718..1314
                     /gene="yjgA"
                     /locus_tag="VM406_14720"
     CDS             718..1314
                     /gene="yjgA"
                     /locus_tag="VM406_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HVL77266.1"
                     /translation="MPNANRGSVGFQSSEFEQKYDRPSKSQLKREMTALQELGAELVA
                     QPKDRVMRVPMPEDVRDAILECQKIRDHEGRRRQLQFVGRKMRTLEDDEIAAIQQVLD
                     SWKGASKAETAAMHALERQREKLLADDKAVTDLLQRHPDADVQHLRTLIRNARKEQAE
                     NKPPKAYRELFQLLKQLKAGAAQADAQTEDDNEAGDAE"
     gene            1304..1912
                     /gene="mog"
                     /locus_tag="VM406_14725"
     CDS             1304..1912
                     /gene="mog"
                     /locus_tag="VM406_14725"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HVL77267.1"
                     /translation="MQNETLRVGLVSVSDRASSGVYEDKGVPALRDWFAAALLTPWEM
                     ETRLIPDERSRIEQALIELVDEARCHLVLTTGGTGPARRDVTPEATLAVGTREMPGFG
                     EQMRQISLKFVPTAILSRQVAVIREIPDHAALIMNLPGQPKSIKEILEGLKDDQGAPL
                     VPGIFAAVPYCIDLIGGPYIETNDDVCKAFRPKSAIRPATST"
     gene            1922..>2302
                     /locus_tag="VM406_14730"
     CDS             1922..>2302
                     /locus_tag="VM406_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HVL77268.1"
                     /translation="MSASLLDAIQIETAPNPAAAVIWLHGLGADGSDFVPLVPELDLT
                     GCPAIRFVFPHAPAMPVTINNGYVMRAWYDILGLDLRTREDEAGLRRSQAMVEALIAR
                     EKARGVPAERIVLAGFSQGCAMSLQ"
BASE COUNT          433 a          754 c          733 g          382 t
ORIGIN      
        1 ctgcatgtcc acgccgcttc gcgcgatcgg cgcaacggac agcgtgtggc gcgaatacgg
       61 atagccaccg atgaagccgc gcgaattcgc gtacacgaac tgcgagtgtt gcgcatgcac
      121 gccggcgcct tcgctattgg cgatgcggcg gtcgacgccg aaggctgcgc cttcgcagcg
      181 ctgggcgagc tcgaccgctt cctcggccgt gacgtcccac ggatggaaca attgcagatc
      241 gcgcggcgcc ttctcgagcg tgtcctcgtc gggcagaccc gcgcagtcgt cctcggcggt
      301 gaatcgagcg atgtcatatg cggcgctcac cgtgtcgcgc aaggacctcg aagagaagtc
      361 ggaggtgctg gcattcccgc gccgcacatg gccgggtgcg cccagatgaa ccgtgactcc
      421 gatccccttg tccttgttct gctcgatcgt ctcgatcttg cccttgcgca cgctgactgc
      481 gagccccttg ccctcgctga tttcgacggc agcatcggag gcgccctttt ccctcgcgaa
      541 acgcaggaca tcctcggcaa tctgccggag ttgctcttgg ctgtgcgtga atagcggatg
      601 cgtcatgggg cgtctggtcg gatcaaagcg ggtatcatag cagtcgttca ttcaagaccc
      661 tgcggcctgg atcaagcgtt gcagcgcgtc ttccactctt ctcacgcact actcaacatg
      721 cccaacgcga accgcggctc ggtcggcttt cagtccagcg aattcgaaca gaagtacgac
      781 cgcccctcca aatcacagct caagcgcgaa atgaccgcgc tgcaggaact cggcgcggaa
      841 ctggtagccc agcccaagga ccgcgtgatg cgcgttccga tgcccgagga tgtgcgcgac
      901 gccatcctcg agtgccagaa gatccgcgac catgaaggtc gtcgccgcca gctgcaattc
      961 gtgggacgca agatgcgcac gctcgaggat gacgaaatcg cggccatcca gcaggtgctc
     1021 gacagctgga aaggcgcatc gaaggccgag acggcggcaa tgcatgcgct cgagcgccag
     1081 cgcgagaagc tgcttgccga cgacaaggcg gtgaccgacc tgctgcagcg gcatccggac
     1141 gccgacgtcc agcacctgcg cacgctgatt cgcaatgcgc gcaaggaaca ggcggagaac
     1201 aagccgccga aggcctaccg cgagctgttc cagctgctca agcagctcaa ggcgggggcg
     1261 gcccaggccg acgcgcagac agaggacgat aacgaggccg gcgatgcaga atgagacgct
     1321 gcgtgtcggc ctggtctccg tctccgaccg ggcctcgtcc ggcgtctacg aagacaaggg
     1381 cgttccggcc ctgcgcgact ggttcgccgc cgcgctgctg acgccatggg aaatggagac
     1441 gcgcctgatt cccgacgaac gcagccgcat cgaacaggcg ctgattgaac tggtggacga
     1501 agcgcgctgc cacctggtac tgacgaccgg cggcaccggc cccgcgcgac gcgacgtgac
     1561 gccggaagcc acgctggccg tcggcacccg cgagatgccg ggtttcggcg aacagatgcg
     1621 ccagatcagc ctgaagttcg tgcccaccgc gattctttcg cgccaggtcg ctgtcattcg
     1681 cgagataccc gatcacgccg ccctcatcat gaacctgccg ggccagccga aatccatcaa
     1741 ggaaatactg gaaggactga aggacgacca gggcgcgccg ctggtacccg gcattttcgc
     1801 tgccgttcct tattgcatcg acctgatcgg cgggccgtat atcgaaacca atgacgacgt
     1861 gtgcaaggcg tttcgtccga agtcggcaat ccgcccggcg acatcaacgt agaggaaaac
     1921 gatgagcgcc tccctgctcg atgcgataca gatagaaacg gcgccgaatc cggccgcggc
     1981 cgtgatctgg ctgcacggtc tgggcgcaga cggcagcgac ttcgtcccgc tcgtgcccga
     2041 gctcgacctg accggctgcc ccgcgatccg cttcgtgttt ccgcacgcgc cggcgatgcc
     2101 ggtcacgatc aacaacggct atgtgatgcg cgcctggtac gacattctcg gcctggatct
     2161 tcgcacgcgc gaggacgagg cgggcttgcg ccgatcgcag gcaatggtcg aagcgctgat
     2221 tgcccgcgag aaggcgcgcg gcgtcccggc cgaacgcatt gtgctggctg gattctcgca
     2281 aggttgcgcg atgagcttgc ag
//
LOCUS       DATLGF010000476        11004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_106438, whole genome shotgun sequence.
ACCESSION   DATLGF010000476 DATLGF010000000
VERSION     DATLGF010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 11004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11004
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106438"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VM406_14735"
     CDS             <1..694
                     /locus_tag="VM406_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL77269.1"
                     /translation="LNDLLARLRQSIGAQRAFIADAAHELRTPLTALQLQIQLAERAQ
                     TPAERAQAFGELKQGYMRAMNMVQQLLTLARQEPDAAEMRREPVALRALAQQAVADHA
                     ALAEERGIDLGIAEAAEVSVAGDPDALRILLGNLVSNALRYTPRGGRVDVHVVKDGHD
                     TVLAVQDTGPGIPEQDLARVFDRFYRVPGTAEVGSGLGLAIAKQIAEAHGALLTLGNT
                     HPGLRAELRWKG"
     gene            1034..2506
                     /locus_tag="VM406_14740"
     CDS             1034..2506
                     /locus_tag="VM406_14740"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HVL77270.1"
                     /translation="MTSKLFKRSVIAVSLAAVMAGGYAGLGGHAAAQTAAPPAAVAAA
                     VPGSAVATDFSGIVKAYGPAVVNISVVGTAREQRARGVPSGPQQGPFGAFPFGPFGPF
                     GPGGPGGAEPAPQARGMGSGFIVSADGMILTNAHVVEGADEVTVRLTDRREFKATVVG
                     ADRQTDVAVLRIDAKNLPTVKIGNPGASAVGEPVLAIGSPYGLENTATAGIISAQSRA
                     LPNDNYVPFIQTDVAVNPGNSGGPLFNLRGEVIGINSQIYSRTGGFQGLSFAVPIDVA
                     IDVKDQLVKHGKVTRGRLGVSVQSITQSLAESLGLNSLNGALVASVERNGPAAKGGLE
                     PGDVIVRFNGQEIRESSDLPARVARMQPGTQVKVEVLRSGKPQALNVTIGQMQDTTVA
                     QRDDEPGAQQQGRLGLAVRPLDRQEQRSAGVQGGVLVEGVSGAAAKAGLERGDIVLSF
                     NGAQVTGPEQLRDLARKSGRNAAVLVQRGEHRLFVPIELG"
     gene            complement(2582..2857)
                     /locus_tag="VM406_14745"
     CDS             complement(2582..2857)
                     /locus_tag="VM406_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77271.1"
                     /translation="MLTISNTRPEAPNVAVFGQLDDGTYAGEVVGETEVPYTRCWDNA
                     IDQVVVYIEPDEKELQEIVKALNDGRLEFRSLQEFGGADGGSSTLPI"
     gene            3074..3835
                     /locus_tag="VM406_14750"
     CDS             3074..3835
                     /locus_tag="VM406_14750"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HVL77272.1"
                     /translation="MLHSAKNLIGFTVHGTDGEVGSVDDVYFDDERWVVRYLVVDTGT
                     WLTGRKVLLSPLSFRQADWDGGTILMNLTQEQIRNSPGIDTHMPVSRQQERELFGHYG
                     YPYYWTGPYAWGFSVEPSLIAEREREDVIANEMREEMAESDDDPHLRSRNEVVGYTIH
                     ATDDTLGHVEDLLFDEEDWSIQLIVIDPRNWWPGKHVMVSPQRISSVNWEDKSVTVGM
                     SRAEIESSPEYDPGNPPLSRPPAMTRDAAGPLRRM"
     gene            3859..5679
                     /locus_tag="VM406_14755"
     CDS             3859..5679
                     /locus_tag="VM406_14755"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HVL77273.1"
                     /translation="MSEHYSYYPKISDYALISDCHCNALISRNGSVDWCCMPRVDDDS
                     IFGRLLDWKKGGYCSIAPEGDFKTSWRYLPETLVLETTFHTATGMARLIDFFAIDPDV
                     DAEPLYDHVRIVQGIEGEVKLRVEVRPRFDYGEILPRMRRAGRSVYTAIGSDKGLIIH
                     ADMELEVAEHDGLYGSATIRAGERLRLALEFETPERVEQRLEEGMASANELDGYFETT
                     RNWWRKWASRMDRRYASDPYTMRSALVLKGLTFERTGAIIAATTTSLPEWIGGERNWD
                     YRYSWVRDSVFTVRVLHDLGYVREADLFHQFIQRSAAGSAEQMQIMYGVDGKRRLTET
                     ILDWMEGYRGSKPVRIGNCAAKQVQLDIYGELLEMAWEWHANGHPTEPDYWTFLRDVV
                     ETACRRWRDADHGIWEVRDVPRHYVHSKVMCWAAVNRGICLAEDNGFEAPLERWRAVR
                     EEMRTAIETEGYDATRNTFVQAFGEPGLDAAVLLLPRVGFLPYDDPRMMGTVDAICQE
                     LDEGGLLLRYNTEDGLEGEEGMFLPCTFWLVACLAQQGRRDRAEKYYRRALECANDIG
                     LFSEEYSIRHEEMLGNFPQGLTHVSQIMAHLALEGNGQGA"
     gene            5692..6792
                     /locus_tag="VM406_14760"
     CDS             5692..6792
                     /locus_tag="VM406_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HVL77274.1"
                     /translation="MRNPIISRMFAMVMLAALLAGCARDDRGPFQAGQTDERTSTAPA
                     SLPSFVELVKQQGPAVVNISTTPAEGETTDPLAEFFRRFGAPGPEAPIAGMGSGFLIS
                     DDGFILTNAHVVADSAEVTVRLTTKREYKARVIGVDTQTDVALIKIEGTGLPVVRVGD
                     SAPVEPGEWVAAIGAPFGFENSITVGVISAKERLLPGGSYVPFLQTDVAVNPGNSGGP
                     LFSSRGEVIGINSMILSQTGGFMGISFAIPIDIAMSVAKELRETGKVTRGRIGVQVQE
                     MTVDLAASFGLKEPEGALIAAVEPASPAQKAGLQAGDVILSMNQEPVESSAGLARFVA
                     NTKPGTTVNAEVWRKGQKLQVPIQVEEMPAAE"
     gene            6905..7318
                     /locus_tag="VM406_14765"
     CDS             6905..7318
                     /locus_tag="VM406_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HVL77275.1"
                     /translation="MNVMRFAFVIGLIYLIVGIAGFVPPLLQSPAPKDPGLAVEALHG
                     RLLGLFPVNVLHTLVHLAIGAWGLFAARTAGAAIFYARALAVIYGLLAVMGLIPGLHT
                     MFGLVPLHSHDVWLHAGTAMLAAYFGWANPGVQTR"
     gene            complement(7364..8404)
                     /locus_tag="VM406_14770"
     CDS             complement(7364..8404)
                     /locus_tag="VM406_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL77276.1"
                     /translation="MSAPVVVVTGASAGIGRATAVAFGRKGYKVALLARGEEGLEGAR
                     RDVEAAGGQAIAIVTDVAEHDQVEAAAERVERELGPIDVWVNNAMATIFAPLEQVAPQ
                     DFERATRVTYLGAVWGTMAALKRMKPRDAGTIVQVGSALAYRSIPLQAAYCGAKSAMR
                     GFTDSLRSELIHDKSRVHVTMVQLGAFNTPQFEWGRTSFDRRPQPVPPIYQPEVAADA
                     IVWAAERRRREVWVGSNAFKAIVGQRLFPGMLDRMMASQAWDGQLDDEPLPRERADNL
                     YTPVQRDYGAHGRFDAQSRPVSWQYRLVKYRWPTLAASVALLLAGGAAWRAGRRAVPG
                     ASDRQARSGRDT"
     gene            8654..9217
                     /locus_tag="VM406_14775"
     CDS             8654..9217
                     /locus_tag="VM406_14775"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HVL77277.1"
                     /translation="MPRGQFSKRLPSAQSLKQSRFLKPFAPYLDHHFLWQFNRRSVAG
                     GVAVGLFFGLLIPFAQIFFAALVAIIIRVNLPTAAFCTLVSNPLTFPAIYYAAYRLGD
                     FLTGAEVEMAEAAIETDIQEVLTVQQGEVIGWFPNLLLWLQSVGLPLALGLLVMSTVG
                     ALAGYFLVSTIWKMNVSRRWRQRRDRM"
     gene            9262..10074
                     /locus_tag="VM406_14780"
     CDS             9262..10074
                     /locus_tag="VM406_14780"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVL77278.1"
                     /translation="MSENRVISLGVVGVALVALADAALNFEISLVALHMLPVLVVTWY
                     TSLRWGMFILVLMTVITVLLTINFAPPASNPLYRYLDLSSDFVAMMLLVFVQSRLREA
                     YRTVRRQSRTDVLTGCLNRSGFHEQLQAEVVRQKRYGHAFSLLYLDVDNFKTLNDTQG
                     HQAGDALLTELGRLLLARLRKTDSAGRLGGDEFAVLLRETGLAAAQHIADEMKAELER
                     TSRGHRWPVGFSIGIVCFSVPPASADEALHRADELMYEAKRGGKNTLRARTV"
     gene            complement(10075..>11004)
                     /locus_tag="VM406_14785"
     CDS             complement(10075..>11004)
                     /locus_tag="VM406_14785"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVL77279.1"
                     /translation="VLADGYACGGGRRGAQQALRNFWKRVSDAARFSPLQRTPMDRML
                     GRWTMDTSPGYVLFGMLTSMISPYEFNPLDVNPLRNLLEDLVDFERVRGCTPLQLFIS
                     ATNVRTGKARIFRREELSVDAVMASACLPQIYRAVEIDGEAYWDGGYMGNPALFPLID
                     ETDAHDLIIVQINPVIREELPRTAPEIMNRLNEITFNSSLIREISSILLLKQLIDEEH
                     VDRVRYTEMRLHRINSEKELLGLSVSSKLNAEWAFLEHLHDVGYRTADDWLRANFDRV
                     GKESTLDTLSMYFAECGVQTPEQPQEQALRRRG"
BASE COUNT         1869 a         3530 c         3695 g         1910 t
ORIGIN      
        1 gctcaacgac ctgctcgcgc gcctgcgcca gtccatcggc gcccagcgcg cgttcatcgc
       61 cgatgccgcc cacgaactgc gcacgccgct gacggcattg cagttgcaga tccagcttgc
      121 cgagcgcgcc cagacgcccg cggagcgcgc gcaggcgttc ggtgaactga aacagggtta
      181 catgcgcgcg atgaacatgg tgcagcagct gctcacgctc gcgcgccagg agccggacgc
      241 cgccgagatg cgccgcgaac cggtcgcgct gcgcgcgctc gcgcagcagg cggtggcgga
      301 ccatgcggca ctggccgagg agcgcgggat cgacctcggc atcgccgagg cggcggaggt
      361 cagcgtggca ggcgacccgg atgcgctgcg catcctgttg ggcaatctcg tctccaatgc
      421 gctgcgttac acgccgcgcg ggggcagggt tgacgtgcac gtggtcaagg acgggcacga
      481 cacggtgctt gccgtgcaag acaccggccc cggcatcccc gagcaagatc tcgcgcgcgt
      541 gttcgaccgt ttctaccggg tgccgggtac ggcggaggtg gggagcggcc tgggactggc
      601 gatcgccaag cagatcgcgg aggcccatgg cgcgctgctg accctgggaa acacgcatcc
      661 gggcttgcgt gcggagttgc ggtggaaggg gtgaggtggc gtgtgaaagc gggttggctg
      721 agggatagcc gttcaaaaat gcgaacaaat tcgcaatgtc gttccggcgc aggcgggaag
      781 ccggctcagc acggtaggct gggatgcaat cccagcaacg agttgggtgg cagaacgatg
      841 ccgggatttt ctccctgcct gcgtccctgc acccgttcaa actgggttcc cgccttcacg
      901 ggaacgacat gacaagagta atcgcttagg cgtgtacgcc ttgcagaacg gcgccggccc
      961 ttttaagtcc attttaagag tccccccgct accatccagc ctatcgtttc gcagcaaccc
     1021 aggagtgcat cgaatgacaa gcaagctttt caagcgcagt gtgatcgccg tttcccttgc
     1081 ggcggtcatg gccggcggct atgccggctt gggcggacat gccgcggccc agaccgcggc
     1141 gccgccggca gcagtggccg ccgccgtacc cggcagtgcc gtcgccaccg atttctccgg
     1201 catcgtcaag gcctacggtc ccgccgtggt gaacatcagc gtggtcggca cggcgcgcga
     1261 gcagcgcgca cgcggcgttc cgagcgggcc gcagcagggt ccgttcggcg cctttccgtt
     1321 cggccccttc ggtccgttcg gaccgggagg acccggcggg gcagagcctg caccgcaggc
     1381 ccgcggcatg ggctcgggct tcattgtcag cgccgacggc atgatcctga ccaatgccca
     1441 cgtggtcgag ggtgccgacg aagtcacggt gcgcctcacc gaccggcgcg agttcaaggc
     1501 cacggtggtt ggagcggacc gtcagaccga cgtcgcggta ctgcgcatcg acgcaaagaa
     1561 cctgccgacc gtgaaaatcg gcaatcccgg cgcatcggcg gtcggcgaac cggtgctggc
     1621 gatcggctcg ccctacggcc tggagaacac ggcgacggcc ggcattatca gtgcccagtc
     1681 gcgcgcgctg ccgaatgaca actatgtgcc gttcatccag acggacgttg cggtcaatcc
     1741 cggcaactcg ggcggcccgc tgttcaatct gcgcggggaa gtgatcggta tcaactcgca
     1801 gatctacagc cgcaccggcg gcttccaggg gctgtcgttc gcggtgccga tcgacgtggc
     1861 gatcgacgtg aaggatcagc tcgtcaagca tggcaaggtc acgcgcggcc ggcttggcgt
     1921 gagcgtacag agcatcacgc agtcgctcgc cgagtcgctt gggctgaatt cgctgaatgg
     1981 cgcgctggtc gcctctgtcg agcgcaacgg gccggcggcc aagggcgggc tggagccggg
     2041 cgatgtgatc gtgcgcttca acggacagga aatccgggaa tcgtcggatc tgcctgcacg
     2101 ggtggcgcgc atgcagccgg gaactcaggt caaggtggag gtgctgcgca gcggcaagcc
     2161 gcaggcgctc aacgtcacga tcggccagat gcaggacacg accgtcgcac agcgcgacga
     2221 cgaacccggc gcccagcagc agggccggct cggacttgcg gtgcgtccgc tcgatcgcca
     2281 ggagcagcgc agcgccggcg tgcagggcgg cgtgctggtc gaaggcgtca gcggtgcggc
     2341 tgccaaggcc ggcctggagc gcggcgacat cgtgctctcg ttcaacgggg cgcaagtcac
     2401 cggtcccgag cagctgcggg acctggcgcg caaatcgggc cgcaacgcgg cggtcctggt
     2461 gcaacggggc gagcaccgcc tgttcgtgcc gatagaactg ggttgatctg ctccagcgga
     2521 gtgcggtgaa gctcccctcg ggcggcaggg gcctctcctg tctcctgtgc tgactctcct
     2581 ctcaaatcgg cagcgtcgac gaccctccgt cggcgccgcc gaactcctga aggctgcgaa
     2641 actccagccg tccgtcgttg agcgccttca cgatttcctg caattccttt tcatcaggtt
     2701 cgatgtagac gacgacctgg tcgatcgcgt tgtcccagca gcgcgtatag ggcacctccg
     2761 tttctcccac gacttcgccg gcataggtgc cgtcgtcgag ctggccgaac acggcgacgt
     2821 tcggcgcttc cgggcgggta ttactgatag taagcatggc ggtcctcgtg gcggcgcaca
     2881 cggtgccact cagaccgtgc cgcgcacccg gaattccatc cggcacggca ggttttacat
     2941 gcgcgcgcat gccggccgcg ccagccctgc ttcacggccg ccgggttcgt cccggcccgc
     3001 attttgcgtg tcgtaaagcg tcggcgcagg cgcgttgcct tgcgtcgcgg aacgcaacgt
     3061 aaggaggaaa tccatgctgc acagtgcaaa gaacctgatc ggcttcaccg tgcatggcac
     3121 cgacggcgag gtcggcagcg tcgacgatgt gtacttcgat gatgagcgct gggtcgtgcg
     3181 ctatctggtg gtcgacacgg gaacctggct gaccgggcgc aaggttctgc tgtcgccgct
     3241 gtcgttccgg caggcggact gggacggcgg gacgatcctg atgaacctca cgcaggagca
     3301 gatccgcaac agtcccggca tcgataccca catgccggtt tcacgccagc aggagcgcga
     3361 actgttcggc cattacggct atccgtacta ctggaccgga ccgtacgcat ggggtttctc
     3421 ggtcgaaccc tcgctgatcg ccgaacggga gcgcgaggac gtgatcgcca acgagatgcg
     3481 cgaggaaatg gccgaaagcg atgacgaccc gcatctgcgc agccgcaacg aggtggtggg
     3541 ctacaccatc cacgccaccg acgatacctt ggggcatgtc gaagatctgc tgttcgacga
     3601 ggaagactgg tcaattcagt tgatcgtgat cgacccgcgc aactggtggc cgggcaaaca
     3661 tgtgatggtg tccccgcaac gcatttcctc ggtgaactgg gaagacaaga gcgtgacggt
     3721 cggcatgagc cgggcggaga tcgagagcag ccccgagtac gatcccggca atccgccgtt
     3781 gtcgcgtccg ccggcaatga cgcgcgatgc ggccggcccg ctgcggcgca tgtagcgcat
     3841 cgaggagacc agcaggcgat gagcgaacac tacagctact acccgaagat cagcgactac
     3901 gcactgatca gcgactgcca ttgcaacgcg ttgatttcgc gcaacggttc ggtcgactgg
     3961 tgctgcatgc cgcgcgtgga tgacgacagt atcttcgggc ggctgctcga ctggaaaaag
     4021 ggcggttatt gctcgattgc gccggaggga gacttcaaga cgagctggcg ctatctgccg
     4081 gaaacgctgg tgctggagac gactttccat accgcgaccg gcatggcgcg cctgatcgat
     4141 tttttcgcca tcgatcccga cgtggatgcc gaaccgctgt acgaccacgt gcgcatcgtc
     4201 cagggcatag aaggcgaggt caagctgcgc gtggaagtgc ggccgcgctt cgactacggc
     4261 gagatcctgc cgcgcatgcg ccgcgccggg cgcagcgtct ataccgcgat cggtagcgac
     4321 aaggggctga tcatccacgc cgacatggag ctcgaggtgg ccgagcatga cggcctgtac
     4381 ggctcggcga cgatccgtgc cggcgagcgg ctgcgcctgg ccctggagtt cgagacgccc
     4441 gagcgcgtgg agcagcgcct cgaggagggc atggcgtccg ccaacgagct ggacggctat
     4501 ttcgagacca cgcgcaactg gtggcgcaag tgggcttcgc gcatggacag gcgctacgcg
     4561 agcgacccgt acacgatgcg ttctgcgctc gtgctcaagg gcctgacctt cgagcgcacc
     4621 ggggccatca tcgccgccac caccacctcg ctgcctgaat ggataggcgg ggagcgcaac
     4681 tgggactacc gctacagctg ggtgcgcgat tcggtgttca ccgtgcgggt gctgcacgat
     4741 ctcggctatg tgcgcgaggc ggatctgttc caccagttca tccagcgcag cgcggccggc
     4801 agcgccgagc agatgcagat catgtacggc gtcgacggca agcgccgcct gaccgaaacc
     4861 atcctcgact ggatggaggg ctaccgcggc tcgaagccgg tacgcatcgg caactgcgcc
     4921 gccaagcagg tccagctcga catttacggc gagttgctgg agatggcgtg ggaatggcat
     4981 gcgaacggcc acccgaccga accggactac tggacctttt tgcgcgatgt cgtcgagacc
     5041 gcctgccgcc gctggcgcga cgccgaccac ggcatctggg aagtgcgcga cgtgccgcgc
     5101 cactatgtgc attcgaaggt catgtgctgg gctgccgtca atcgcggcat ctgtctggcg
     5161 gaggacaacg gcttcgaggc gccgctggag cgctggcgcg cagtgcgcga ggaaatgcgc
     5221 acggcgatcg aaaccgaggg ttacgacgcc acgcgcaata cctttgtgca ggcattcgga
     5281 gagccggggc tggacgcggc cgtgctgctg ctgccgcgcg tgggcttcct gccgtatgac
     5341 gatccgcgca tgatgggcac ggtcgacgcg atctgccagg agctcgacga aggcggcctg
     5401 ctgttgcgct ataacaccga ggatgggctg gaaggcgagg aaggcatgtt cctgccgtgc
     5461 acgttctggc tggttgcctg cctggcgcag cagggtcggc gcgaccgcgc cgaaaagtac
     5521 tacaggcgcg cgctggaatg tgcgaacgac atcggcttgt tttccgagga atacagcatc
     5581 aggcatgaag aaatgctggg caactttcca cagggcttga cccatgtctc gcagatcatg
     5641 gcccatctgg ccctcgaagg taacgggcag ggcgcttaaa ggagaagccg agtgcgcaat
     5701 ccaatcattt cccgaatgtt cgcaatggtg atgcttgccg cgctgctggc cggttgcgca
     5761 cgcgacgaca gggggccgtt ccaggccggc cagaccgacg agcggaccag cacggccccc
     5821 gccagcctgc ccagctttgt ggagctggta aagcagcagg ggccggccgt ggtcaacatc
     5881 agcaccactc cggccgaagg cgaaaccacc gatccgctcg cggaattttt ccgccgcttc
     5941 ggcgcgccgg gtccggaagc cccgatcgcg gggatgggat cggggttcct gatctccgac
     6001 gacggcttca tcctcaccaa tgcccacgtg gtcgccgaca gcgccgaggt gacggtgcgc
     6061 ctgaccacga aacgcgaata caaggcgcgc gtgatcggcg tcgatacgca gaccgacgtg
     6121 gcgctgatca agatcgaagg caccggactg ccggtggtgc gtgtgggtga ttccgcgccg
     6181 gtggaaccgg gcgaatgggt ggccgccatc ggcgccccgt tcggcttcga aaacagcatc
     6241 accgtgggcg tgatcagcgc gaaggaacgc ctgctgcccg gcggaagcta cgtgcccttt
     6301 ctgcagaccg atgtggcggt caaccccggc aattccggcg ggccgctgtt ctcgtcgcgc
     6361 ggcgaggtga tcggcatcaa ctcgatgatc ctgagccaga ccggcggctt catgggtatt
     6421 tccttcgcca ttccgatcga catcgcgatg agcgtggcca aggaattgcg tgagactggc
     6481 aaggtcacgc gcggccgcat cggcgtgcag gtgcaggaaa tgacggtcga cctggccgcg
     6541 tcgttcggac tgaaagagcc ggaaggggcg ctgatcgccg cagtggagcc ggcatcgccg
     6601 gcacagaagg ccggcctgca ggccggcgac gtgatccttt cgatgaacca ggaaccggtc
     6661 gaatcttcgg cgggacttgc gcgcttcgtc gccaatacca agcctggcac caccgtcaat
     6721 gccgaggtct ggcgcaaggg gcagaaactg caggtgccga tacaggtcga ggagatgccg
     6781 gcagcggagt agcggcgtca cgctgcacgg cggccggcgc gagtccggtc acacgcgcgg
     6841 caaacgtggc gcaggaaagc aggctttgcc gttgttgttt ccacatttac tttctggagg
     6901 ggccatgaac gtgatgcgct ttgcctttgt gatcggattg atctacctga tcgtgggaat
     6961 tgccggattc gtgccgccat tgctgcagtc gcccgccccg aaggacccgg gcctggccgt
     7021 ggaagccctg cacggacgcc tgctgggact gttcccggta aacgtgctgc acacgctggt
     7081 gcatctcgcg atcggggcat ggggactgtt cgccgcccgc accgccggcg cggcgatttt
     7141 ttatgcgcgc gccctggccg tcatctacgg gctgcttgcc gtcatgggtc tgattcccgg
     7201 cctgcacacg atgttcggct tggtgccgct gcacagccat gacgtctggc tccacgccgg
     7261 taccgcgatg ctggcagcgt acttcgggtg ggcgaatccc ggcgtgcaga cgcgctgaca
     7321 tcgcttcgtc gaaggaacgg cgcccgcacc gttccttctc cgatcaggta tcgcgcccgg
     7381 agcgtgcttg acggtccgac gcgcccggca ccgcgcggcg cccggcgcgc catgcggccc
     7441 cgccggccag cagcagcgca accgacgcgg ccagcgtggg ccagcggtac ttgaccagcc
     7501 ggtactgcca gctgaccggc cgcgattgcg catcgaagcg gccgtgcgcc ccgtagtcgc
     7561 gctgtaccgg ggtgtacaga ttgtcggccc gctcccgcgg cagcggctcg tcgtcgagct
     7621 gcccgtccca ggcctggctg gccatcatcc ggtcgagcat gccggggaac agacgttgcc
     7681 cgacaatcgc cttgaacgcg ttggatccga cccagacctc gcggcgcctg cgctcggcgg
     7741 cccagacgat cgcatcggcg gccacttccg gctggtagat aggcggcacc ggctgcggac
     7801 gccggtcgaa gctggtgcgg ccccactcga actgcggcgt attgaacgcc cccagttgca
     7861 ccatcgtcac gtgcacccgg ctcttgtcat ggatcaattc cgagcgcagc gaatcggtga
     7921 agccgcgcat tgcgctcttg gcgccgcagt aggcggcctg cagcggaatc gaacggtagg
     7981 cgagggctga cccgacctgg acgatggtgc cggcgtcgcg cggcttcatg cgcttcagtg
     8041 cagccatcgt gccccagact gcccccaggt aggtgacccg ggtcgcgcgc tcgaaatcct
     8101 gcggcgcgac ctgctcgagc ggcgcaaaga ttgtcgccat cgcattgttg acccacacgt
     8161 cgatcggtcc cagttcgcgc tccacgcgct cggcggccgc ctccacctgg tcgtgctcgg
     8221 ccacatcggt cacgattgcg atcgcctggc cgccggccgc ttcgacatcc cggcgggcgc
     8281 cttcgaggcc ttcctccccg cgcgcgagca gggcgacctt gtagcctttc ctgccgaacg
     8341 ccaccgccgt cgcgcggccg attccggcgg acgcgccggt caccacaacc accggggcgc
     8401 tcattgcaca cccccgttcc cggcgcatgc gccggtcatt gccatcctga tcatcttgtt
     8461 cattctcggc tccatcctga tttctgttag gagtccgggc aacttcgcgg agttctgcgc
     8521 ccggaacgca cttgagctgc atcacgatgc cagaaggtct acacgtttcc agaacaaggc
     8581 gcgttgcggc aacgaatcgg gtcggcctcg gcatacaatg ggcgctgctc caatcaatgc
     8641 agggatattg cgcatgccgc gaggccagtt ttccaaacgt ctgccgtccg cccagtcact
     8701 gaagcaaagt cgcttcctga aaccttttgc cccttatctg gatcatcact ttctctggca
     8761 gttcaaccgg cgctcggtgg ccggcggggt ggccgtgggt ctgttcttcg ggcttctgat
     8821 tccatttgcg cagatcttct tcgccgcgct ggtcgcgatc atcatccggg tcaacctgcc
     8881 gacggcggct ttctgcacgc tggtgtcgaa tcccctgacg ttcccggcga tctattacgc
     8941 cgcgtaccgg ctcggcgatt tcctgaccgg ggcggaagtc gagatggccg aggcggcgat
     9001 agagacggac atccaggaag tgctgaccgt gcagcagggc gaggtgatcg gctggttccc
     9061 gaacctgctg ctgtggctcc agagcgtcgg cctgccgctg gcgctcgggc tgctggtgat
     9121 gtccacggtg ggcgcgctgg ccggctactt cctggtcagc acgatctgga agatgaacgt
     9181 gtcgcgccgc tggcgccagc ggcgcgacag gatgtagcga acgcatagca gcgaagaggg
     9241 aggtgaggcg aggcgatgac gatgagcgag aatcgggtga tcagccttgg tgtggtcggc
     9301 gtcgcgctgg tcgcacttgc ggacgcggca ctgaacttcg agatcagcct cgtcgcgctg
     9361 cacatgctcc cggttctcgt cgtcacctgg tacacctcgc tgcgctgggg gatgttcatc
     9421 ctcgtgctga tgacggtcat cacggtgctg ttgaccatca atttcgcgcc gcccgcgtcc
     9481 aatccgctgt accgctacct tgacctgtcc agcgatttcg tcgcgatgat gctgctcgta
     9541 ttcgtgcagt cgcggctgcg cgaagcctac cggaccgtca ggcgccagtc gcgcaccgat
     9601 gtgctgaccg gctgcctgaa ccgcagcggc tttcatgaac agctgcaggc ggaagtcgtg
     9661 cgccagaaac gctatggcca tgcgttttcg ctgctctacc tcgacgtcga caatttcaag
     9721 acgctcaacg acacccaggg gcaccaggcc ggcgatgcgc tgctgaccga gctgggacgc
     9781 ctgttgcttg cccgcctgcg caagacagac agcgcgggcc ggctcggcgg cgacgagttc
     9841 gcggtgctgc tgcgcgaaac cggcctggcc gccgcccagc acatcgccga cgagatgaag
     9901 gccgagcttg aacgcacgtc ccgcgggcac cgctggccgg taggcttcag catcggcatc
     9961 gtctgtttca gcgtgccccc ggccagcgcc gacgaggcgc tgcaccgggc cgacgagctg
    10021 atgtacgaag cgaagagggg cgggaagaat acgctgcgcg cgcgcaccgt ctgatcagcc
    10081 gcgcctgcgc agtgcctgtt cctgcggctg ctccggcgtc tgcacgccgc attcggcgaa
    10141 gtacatcgac agcgtgtcca gcgtactttc cttgccgacg cggtcgaaat tggcgcgcag
    10201 ccagtcgtcg gcggtgcggt agccgacgtc gtgcagatgt tcgaggaagg cccactcggc
    10261 gttcaacttg ctcgacaccg acagtcccag caattccttt tccgagttaa tccggtgcag
    10321 acgcatttcg gtgtagcgca ccctgtccac atgttcctcg tcgatcagct gcttgagcag
    10381 caggatcgag ctgatctcgc ggatcaggct tgagttgaac gtgatttcat tgagccggtt
    10441 catgatctcg ggcgcggtgc gcggcagttc ttcgcggatg accgggttga tctgcacgat
    10501 gatcagatcg tgcgcatcgg tctcgtcgat cagcggaaac agcgccggat tccccatgta
    10561 gccgccgtcc cagtaggcct cgccgtcgat ttccaccgcc cggtagatct gcggcaggca
    10621 ggcggaggcc atcaccgcat ccacgctcag ttcctcgcgg cggaagatgc gcgccttgcc
    10681 tgtgcgcacg ttggttgcag agatgaacaa ctgcagtggt gtgcagccgc gcacgcgctc
    10741 gaaatccacc aggtcctcca gcaggttgcg cagcgggttg acgtcgagcg gattgaattc
    10801 gtaaggcgag atcatgctgg tgagcatgcc gaacagcaca tagccgggcg aggtgtccat
    10861 cgtccagcgt ccgagcatgc ggtccatcgg cgtgcgttgc agcgggctga agcgggcggc
    10921 gtcgctcacc cgtttccaga aattgcgcag cgcctgctgg gcgccacggc gtccgccgcc
    10981 gcaggcgtag ccgtcggcca ggac
//
LOCUS       DATLGF010000477         3987 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_14181,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000477 DATLGF010000000
VERSION     DATLGF010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14181"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1507)
                     /locus_tag="VM406_14790"
     CDS             complement(<1..1507)
                     /locus_tag="VM406_14790"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HVL77280.1"
                     /translation="MDQLAAHVNHGDHLCVVCADANDRLAAATQYIADGLARNEFVMY
                     AADEEVADAIRGRLVALGVDVAGAERRGALKLPTAEHAYLRNGAFDADGMYAEFDQTI
                     SEALAAGFTGCRFAGEPNWAIRYPDLRTGLIEFESRLNGLFRHRKAAGLCVYDRTAWP
                     ASAIRDVLRTHPVAVVDDLVCTRNLYYERPDLIDTHVSAEHQVSWMLSQLRELRLQEA
                     RLQIALEAGRLGSWELDLATDTSVRSLQHDQIFGHDHLLPDWGYARFMEHVLPEDRER
                     VDAALRAALQTGATWHFECRIRRHGDGAVRWIEAYGRPDSQRAPDGSILRLLGIVADI
                     TERKDMEEALRENDRRKDEFLATLSHELRNPLAPIQNALQFMRLQSRGDAKLQGMQDV
                     IERQVRHLSRLVDDLLDVSRIATGRIVLQCERVELASVLASAVEASQPLIDAAGHRLA
                     MSLPDMQVWLNGDPVRLAQVFLNLLNNAAKYTPAQGSIEVSARFDGREVVVR"
     gene            1702..3189
                     /locus_tag="VM406_14795"
     CDS             1702..3189
                     /locus_tag="VM406_14795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL77281.1"
                     /translation="MHSHLPPAIATLQSRVSDIVERYIAPEAAAVDAEPLEPMPLNVS
                     RRVADMGDLLQRALGQDVELETVLSARLWTTLIDPHQLENVILNLAINSRDAMPEGGK
                     LTIETANTTLDESYVSEYPDLAPGQYVMLAVSDTGHGMSPEVLERAFEPFFTTKPEGK
                     GTGLGLSMAYGFAKQSGGHIRIYSELGHGTTVKLYLPRSHEKEIVLPHRGTGPVERGS
                     ETILVVEDDLAVQAAVVDMLQSLGYKVLRANEAQAAMTILESGVHVDLVFTDVVMPGP
                     MRSTELAQRAKALFPDIAVLFTSGYTQNAIVHGGRLDPGVELLSKPYRQEDLGRKVRQ
                     LLARRQPAGSRADATTALPTPAPASTAVPPAPTGTASRPLHVLVVEDQADLRDTTVQL
                     LEILGCVAIAVPTAEAADGVLATRNFDLLITDVRLPGRSGIELAQQARKQHQNMMVVL
                     CSGYGAALDPPAGLDAEFLPKPYSVPQIAALLDRVRATSAPPVRT"
     gene            complement(3362..3437)
                     /locus_tag="VM406_14800"
     tRNA            complement(3362..3437)
                     /locus_tag="VM406_14800"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3402..3404),aa:Arg,seq:ccg)
     gene            3590..>3987
                     /locus_tag="VM406_14805"
     CDS             3590..>3987
                     /locus_tag="VM406_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVL77282.1"
                     /translation="MSDSTQEEAHTGPIKTPKQLLVVVFLSFVVPVLIIMGLVAYVAS
                     DNKPAGTTEVESYALGGVTAQDVERGVAERIRKVGTVEIRDANRPLKAGAEVYKAQCA
                     ACHDQAVAGAPKMGDQGAWAARIRTGFDAT"
BASE COUNT          696 a         1320 c         1319 g          652 t
ORIGIN      
        1 cccggacgac gacctcgcgg ccgtcgaacc gggccgaaac ctcgatcgac ccctgcgccg
       61 gcgtgtactt cgcggcgttg ttgagcaggt tgaggaacac ctgcgcgagg cgcaccggat
      121 cgccattcag ccagacctgc atgtccggca gggacatggc taagcggtgc cccgcggcat
      181 cgatgagcgg ctgactcgcc tccaccgcgc tggccagcac gctcgccagc tcgacgcgtt
      241 cgcactggag cacgatgcgg ccagtggcaa tccgcgaaac gtcaagcagg tcgtccacca
      301 gccgggacag gtggcgcacc tggcgctcga tcacgtcctg catgccttgt agcttcgcgt
      361 cgccgcgcga ctgcagcctc atgaactgga gcgcgttctg gatcggcgcg agcggattgc
      421 gcaactcgtg cgagagcgtc gcgaggaact cgtccttgcg gcggtcgttc tcgcgcaacg
      481 cctcctccat gtccttgcgt tctgtgatgt ccgcaacgat gcccagcagc cgcaggatgc
      541 tcccgtcggg cgcccgctgg ctgtccgggc ggccataggc ctcgatccac cgcaccgcgc
      601 catcgccgtg gcgtcggatc cggcactcaa agtgccacgt cgctcccgtc tgcagcgcgg
      661 cgcgcagcgc ggcatcgacg cgttcgcggt cttcgggaag gacgtgctcc atgaaccggg
      721 cgtatcccca gtcgggcagg aggtggtcgt ggccgaagat ttggtcgtgc tgcagggagc
      781 gcaccgaggt gtcggtcgcg agatcgagct cccaactccc gagcctgccg gcctccagcg
      841 cgatctgcag ccgcgcttcc tgcagtcgga gttcgcgcag ctgcgagagc atccagctca
      901 cttggtgttc ggccgaaacg tgggtgtcga tcaggtccgg ccgctcgtag taaaggttgc
      961 gtgtgcagac gaggtcgtcg accaccgcca caggatgggt gcgcagaacg tcgcggatgg
     1021 cggacgctgg ccacgcggta cggtcgtaga cgcacaggcc cgctgccttg cgatgccgga
     1081 acagcccgtt caggcgcgac tcgaactcga tcagcccggt tcgcaggtcc ggatagcgga
     1141 tcgcccagtt cggttcgcca gcgaagcggc agcccgtgaa gccggccgcc agcgcctccg
     1201 agatcgtctg gtcaaattcc gcgtacatcc cgtcggcatc gaaggcgccg ttgcgcaggt
     1261 acgcatgttc ggccgtgggt agcttcagcg cgccgcggcg ctccgcaccc gccacgtcca
     1321 cacccagggc gaccagtctg ccgcggatgg cgtcggccac ttcctcgtcc gccgcataca
     1381 tgacgaactc gttgcgagcc aggccgtcgg cgatgtactg ggttgccgcg gcgagccggt
     1441 cgttcgcgtc ggcgcagacc acgcacagat ggtcaccgtg gttcacatgg gcggcgagtt
     1501 ggtccatgag ggtctgcgac ggagtgtgct gtgcaagcat ggcggctgac tcaagagcga
     1561 ggggtggatt ctcgcagagc cacgctgagg gctggggcag atccattgct gggttgggcg
     1621 gaactcgctt gcggatgggc tgccgtccta catggccgat ggaagcgggc ccacatactc
     1681 ggcccgcacc accgggcccc catgcattca catctccctc cagcgatcgc cacactccag
     1741 agccgcgtct cggacatcgt cgagcgctac attgcgcctg aggctgctgc cgtggatgcc
     1801 gagccgttgg aacccatgcc gctgaacgtg agccggcgag ttgccgacat gggcgatctg
     1861 ctgcagcggg cactcggaca agacgtcgag cttgagacgg tgctttcggc gaggctttgg
     1921 accacattga tcgaccccca ccagctcgag aacgtcatcc tgaacctcgc catcaactcg
     1981 cgtgacgcga tgcccgaggg ggggaagctc accatcgaga cggcgaacac cactctggat
     2041 gagtcctatg tcagcgaata tccggatctg gcgccaggcc agtacgtaat gcttgccgtg
     2101 tccgatacgg gacatgggat gagccccgag gtcctggagc gcgcattcga gccgttcttc
     2161 acgaccaagc cggaaggcaa gggcacgggc ctgggcctga gcatggcata cggctttgcc
     2221 aagcaaagtg gcggccacat tcgcatctac agtgaactcg gccatggcac gacggtgaag
     2281 ctgtatctgc cacgttccca cgaaaaggag atcgtgcttc cccatcgcgg cacgggccct
     2341 gtggaacgcg gttcggaaac gatcctcgtc gtagaggacg acctggccgt gcaagcggcg
     2401 gtggtcgaca tgctgcagtc gctgggctac aaggttctcc gcgccaacga ggcgcaagct
     2461 gcgatgacga tcctcgagag cggggttcac gtggatctgg tcttcaccga tgtcgtgatg
     2521 ccagggccga tgcgcagcac cgagctggcc cagcgcgcaa aggcgctgtt tcccgacatc
     2581 gccgtgctgt tcacgagcgg ctacacgcag aacgccatcg tccacggcgg gaggctcgat
     2641 cctggcgtgg agctgctcag caagccctac cgccaggaag acctggggcg caaggtgcgc
     2701 cagctgctgg cgcgtcgcca gccggccggg agccgagctg atgcaacgac tgcattgcca
     2761 acgcccgctc cggcttcgac agcagttccg ccagcaccga ctgggaccgc atcgcggccg
     2821 ctgcacgtcc tggtggtgga agaccaggcc gacttgcgcg acaccaccgt gcaactgctg
     2881 gaaatcctcg gctgcgtagc cattgccgta cccaccgccg aggcggcaga tggggtcctg
     2941 gcgaccagga acttcgacct tctgatcacc gacgttcgct tacctgggcg cagcggtatt
     3001 gaacttgctc agcaggcgcg caagcagcac cagaacatga tggtcgtgct gtgctctgga
     3061 tatggagccg cgctggaccc gcctgccgga ctcgatgcgg agttcctccc caagccctac
     3121 tcagtgccgc agatcgccgc gttgctggat cgcgtgcggg caaccagcgc gccgcctgtg
     3181 cggacctgaa gggcgcgccg actacaccgc gtagccgcac gaggaccgtc agtcgggcga
     3241 ggtagatgcc gctctcggcc gcagctcaag gttctgcata tccgaccctt tgcgctaggg
     3301 tggtgcagca agccgggcct gatcgagaaa gcccaatgaa atcaacccgt ttagcgagtt
     3361 gtggcgcggc tggcggggat cgaacccacg acccttggct tcggaggcca atactctatc
     3421 cactgagcta cagccgcatc agggcaggcg cgcattatcg cccatcgcgg ccctcgcctg
     3481 ggcgagggcc gcagcgcacg gtccggccag gaagggccgc aggagggcga cagctataat
     3541 cgaaggttgt ttttgaccca cccagcacta gaaatttctt gaggacgcca tgagcgacag
     3601 cacgcaggaa gaagcccaca ctggcccgat caagacgccc aagcagttgc tggtggtcgt
     3661 gttcctgtcg ttcgtggtgc cggtgctgat catcatgggc ctggtcgcct acgtggcctc
     3721 cgacaacaag ccggcgggca ccaccgaagt cgagtcctac gccctcggcg gcgtgacggc
     3781 gcaggacgtg gagcgcggcg tcgccgagcg catccgcaag gtcggcaccg tcgagatccg
     3841 cgacgccaac cgaccgctca aggccggcgc cgaggtctac aaggcccagt gcgccgcctg
     3901 tcacgaccag gccgtcgccg gcgcgccgaa gatgggcgac cagggtgcct gggccgcgcg
     3961 catccgcacg ggtttcgacg cgaccag
//
LOCUS       DATLGF010000478         3568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_14429,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000478 DATLGF010000000
VERSION     DATLGF010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14429"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1031)
                     /locus_tag="VM406_14810"
     CDS             complement(138..1031)
                     /locus_tag="VM406_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77283.1"
                     /translation="MFRPLIISVLLATSASAWARIGPEIAYSTQTEVYLVNADGSGKV
                     RLHRSKSNDFISSVALKPGGGTIAFVENWTLKFLDYNSAGQPVGTIRTIRPSCYRLAD
                     VHFHPDGNSVLYWEVCGNDGVVKRVAVPTSANPNPLPETLFTVTDLQELGHWEPGGNS
                     FLYTVLTPTSIELRRRYTDGTADPANPIASSTHHIKYPEVSGSGDRILISDSPLAAGA
                     YPGPGYTSEIDSSGSVVRPNFISGRMGRYAPGNDPRIVFIFQKSYNERYLQYLDGSGQ
                     VRRIGGKGVYVSVDWGEASAR"
     gene            1157..2275
                     /locus_tag="VM406_14815"
     CDS             1157..2275
                     /locus_tag="VM406_14815"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HVL77284.1"
                     /translation="MLDKYSATVDTIYAAATGSARWEDALHAVEELTDSAGAVIGFVP
                     KQESAAAFNLAGRFTSEQCATYSKQYQPICRRTRFMMDHPDLSFVCDSMLITETEMNT
                     DPVYDWFGQHDLRYFIGGSLPETQQHRPVWSLQRSPAQGHVQRKDVHLFNLLTPHMGR
                     ALTLADQIGTLRAFQRFSSAMLEALPQAVFALDAQGRLLFANGPGGALLGCADGLTAG
                     AGHLRTASSTEQSKLDQMIRSAIAPLDGPAGGWTRVSRPSGRLPYAVFVAPLSFADDE
                     LAAAEAKVLVMVHDTGDHRSADPDMLTSLYCLTETEARLASALSAGHSVESAAALLHI
                     QPSTARAHLKHVFNKVGVNRQQDLVRLLTSLSALSPPA"
     gene            2319..2915
                     /locus_tag="VM406_14820"
     CDS             2319..2915
                     /locus_tag="VM406_14820"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HVL77285.1"
                     /translation="MVPRHAGKLALWALLAVPAALILHEHWTSPDLWPGDFLPATGEW
                     SARLIIAALMLTPLQQLLPRSSAVRWLVRHRRAIGVAAFAYALLHLAFYVLDMETLAN
                     MLAELGAPSIWTGWLALLCLLPPALTSNDAAVRALRGAWKRLQRLAHPAAVLTLIHWV
                     LVHDGMVEAILHFLPLALLQIARLARIFTLIPAERKIA"
     gene            2912..3220
                     /locus_tag="VM406_14825"
     CDS             2912..3220
                     /locus_tag="VM406_14825"
                     /inference="COORDINATES: protein motif:HMM:NF025051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HVL77286.1"
                     /translation="MTLRLLTLAMAAAGIAAAATAPASATGKMTCDAPQAQWKGIPAL
                     EAQLKKDGWEIRKSKVDGGCYEVYGTDPDGNRVEAYFHPVTFKKLLVSRRGEILFRAN
                     "
     gene            3348..>3568
                     /locus_tag="VM406_14830"
     CDS             3348..>3568
                     /locus_tag="VM406_14830"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HVL77287.1"
                     /translation="MNGWVSRNDRQDARIDAVVHRTDGDKVNVTLANLSYEGCRIESG
                     EMFRIGERVRIAVPRVGSIDAQVRWALEG"
BASE COUNT          633 a         1138 c         1129 g          668 t
ORIGIN      
        1 gatcgaaacc gtcgagctgg cgacgaccct ggcgacgctc ggctgcgcga gcggccaggg
       61 ctattatttc gccaagccgc tcgaggccga agcggcgctc gattattggc ggtcgcgccg
      121 gcgcagctga gccgacctca cctggcgcta gcttctcccc aatcgaccga cacgtagacg
      181 cccttgcccc cgatccgcct tacctgtccg cttccatcca aatattggag gtagcgctcg
      241 ttgtaggatt tctggaaaat gaaaacgatc cgtgggtcat tccccggcgc ataacggccc
      301 atgcgtcccg aaatgaagtt cgggcggacg acggagccgg aggaatcgat ctcactggtg
      361 taaccggggc caggataggc gcccgccgcc aagggagaat ccgagatcag gattcgatcg
      421 ccggagccgg aaacctcagg atatttgatg tggtgcgtcg agctggcgat cgggttcgca
      481 gggtccgccg tgccgtcagt gtagcgacga cgaagctcta tgctcgtggg tgtcagaacg
      541 gtgtataaga atgaattccc tcccggctcc caatgcccca gctcctgaag atcggttacc
      601 gtaaaaaggg tttccggtag cgggttggga ttggcgctgg tgggcactgc gacgcgcttc
      661 acgaccccat cgttgccgca gacctcccaa taaagaacag agttgccgtc aggatggaag
      721 tggacatcgg caaggcggta acagctcggc ctgatcgtcc gtatcgtccc gactggctgg
      781 ccggcggaat tgtagtcgag aaacttgaga gtccagtttt cgacgaacgc gatcgtcccc
      841 ccgcccggtt taagggcgac gctgctgatg aaatcattgg atttgctgcg atgcagccta
      901 accttgccgc tgccgtcagc gttgacgagg tacacttccg tttgcgtcga atatgcgatt
      961 tccgggccga tgcgagccca agccgacgcg ctggttgcga gcagtacgga gatgatcagc
     1021 ggacggaaca ttgcctttct ccccagttcg agcggcacgc ctggcgagcc acgcccaaat
     1081 acacatcaat ttacttttga tttaatcggt ggttacatcc cctgtttggg gtaggcatcg
     1141 gcgcgggagt ggccgcatgc tggataagta cagcgccact gttgacacga tctatgcggc
     1201 cgccaccggg agtgcacggt gggaagatgc gctgcacgcc gtcgaggaac taaccgacag
     1261 cgccggggcg gtcatcggct tcgtgccgaa gcaggagagc gcggccgcct tcaacctggc
     1321 gggccgattc accagcgaac agtgcgcgac ctattccaag caatatcagc ccatctgccg
     1381 gcgcacccgc ttcatgatgg accacccgga tctaagcttc gtctgcgact ccatgctgat
     1441 caccgaaacg gagatgaata ccgacccggt ctatgattgg ttcgggcagc acgaccttcg
     1501 ctactttatc ggcggttcct tgcctgagac acagcagcac cggcctgtct ggtcgctcca
     1561 gcgcagcccg gcgcaaggcc atgtccaacg gaaggacgtc catcttttca acctgctgac
     1621 cccgcacatg ggccgtgcat tgacgctcgc agaccagatc ggcacgcttc gagcattcca
     1681 gcggttcagc tcggcaatgc tggaagcgct gccgcaggct gtattcgcgc ttgatgccca
     1741 ggggaggctg ctgttcgcca atggcccggg cggagcattg ctgggctgcg ccgacggctt
     1801 gactgccggg gctggccatc tccggactgc cagttcaacc gagcagtcga aactggacca
     1861 aatgatacga agcgcgatcg cgccgctgga cggcccggcc ggcggctgga cgcgcgtgtc
     1921 gcgcccgtcg ggacggctgc cttacgcggt ctttgttgcg cctctatctt ttgcggacga
     1981 tgagctggcc gcggccgagg cgaaggtgct ggtgatggtc catgacaccg gagatcatcg
     2041 ctcggccgat ccggacatgc tgaccagctt atactgcttg acggagacgg aggcacgcct
     2101 ggctagcgcc ctgtcggccg gccacagcgt cgaaagcgcg gcggcgctgc tgcacattca
     2161 gccgtcgacg gcgcgtgccc acttgaagca cgtcttcaac aaggtggggg tcaatcggca
     2221 gcaggacctg gttcggcttt tgacgtcgct gtcggcgctt tcaccgccgg cctgacgttc
     2281 actgttgcga atcgctcgca ataagctatg acgggcgcat ggttccgcgt catgcgggca
     2341 agcttgcgct gtgggccctg ctcgcggtgc cggcggccct gatcctccac gaacactgga
     2401 cctcgccgga cctgtggccg ggcgactttc tgccggcgac gggcgaatgg tcggcgcggc
     2461 tgatcatcgc cgcgttgatg ctcactccgc tacagcagct gctgccgcga agctcggcgg
     2521 tgcggtggtt ggtccgccac cggcgggcca tcggcgtcgc agcgtttgcc tatgccctgc
     2581 tccatctcgc cttctacgtc ctcgacatgg agaccctggc caacatgctg gccgagctcg
     2641 gggcgccgtc aatctggacc ggctggcttg cgcttctgtg cctgctgccg ccggcgctga
     2701 ccagcaatga cgcggcggtg cgggcgctca ggggcgcgtg gaagcggctt caacgtttgg
     2761 cccacccggc ggcggttctg accctcatcc actgggtgct cgtccacgac ggaatggtcg
     2821 aggccatcct ccatttcctg ccgctcgccc tgctccagat cgcccggctg gcgcgcatct
     2881 tcaccctcat ccctgcagaa aggaaaatcg catgacctta cgccttctca cgctcgccat
     2941 ggccgcggcc ggcatcgccg ccgccgccac ggctcccgcc tccgcgaccg gcaagatgac
     3001 ctgcgacgcg ccgcaggcgc agtggaaggg tattcccgcg ctcgaggcgc agctgaagaa
     3061 ggacggctgg gaaatccgca agtccaaagt cgacggcggc tgctacgaag tctacggcac
     3121 cgaccccgac ggcaatcggg tcgaagccta tttccacccg gtgacgttca agaagctgct
     3181 ggtctcgcgc cgcggcgaga ttctgttccg cgccaactga cctggagagc gcgccgccgg
     3241 caaaatggct cgcggcgcgc gttactgatt aatcctcttt ttacccgggc gtagctatcg
     3301 tccccagcga ctgcggctag tcgtggggta ttgcaatgca gcaacgcatg aacggctggg
     3361 tgtcgcgaaa cgaccgccag gacgctcgaa tcgacgccgt cgtgcaccgc actgacggcg
     3421 acaaggtgaa cgtcaccctc gccaaccttt cctacgaggg ctgcaggatc gaatccggcg
     3481 agatgttccg gattggcgag cgagtccgca tcgccgtgcc acgggtcggg tcgatcgacg
     3541 cccaggtgcg ctgggcgctc gagggcag
//
LOCUS       DATLGF010000479         7817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_14585,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000479 DATLGF010000000
VERSION     DATLGF010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7817
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14585"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..3340)
                     /locus_tag="VM406_14835"
     CDS             complement(221..3340)
                     /locus_tag="VM406_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVL77288.1"
                     /translation="MFKWLLDNSLSNRLLVIIASLVLMAYGAFTLSQTPVDVFPDLNK
                     PTVTIMTEAGGMAAEEVEQLITFPLETTMNGLPGVESVRSVSSAGLSFIYVTFDWSTE
                     IFRARQMVSERLSSMEEGLAADVVPRMGPISSIMGEIMQVAIPVDTAKISLMEVREYA
                     DWVLRPRLMAIPGVAQVIPIGGEVRQFQVQPDTTRMAALGITHDDLEAALKGYSANTS
                     GGFLELNGREYLIRMLGQTSRLEDLKNLALTAREGQPVLLRQIADVTFAAATRRGDAG
                     FEGKPAVILGIQKQPTADTISLTRSIEAALEEMNKGLPPGMEAPRVTFRQASFIESSI
                     NTLQGKLIGASVFVAAILYLFLGTIRPTLIALTAIPVSIFITALVFKYFGMSINTMTL
                     GGLAIAIGGLVDDAVVGVENVMRRLKVDRDKHPEHRLGPLQLVAHATMEVRSAILYAT
                     IIIVLVFLPLFALPGMEGRLFVPLGVAFIVSTLASLVVSVTVTPVLAYYLLPGMKSLD
                     HGDTRLLAWLKKRYRSGLQSVLNHPKAAVGTGAAAVLIAAAAVPFFPTSFLPPFNEGT
                     LLIGLRLNPGVTLAESSALARQAEVLVSQVPEVTHVGRRSGRAELDEHAEGVHVSELD
                     VGLKPADELERSMDEIQADIRSRIAHLPAAIAIGQPISHRIDHMLSGVRSQIAIKIFG
                     EDLDTLRGQADLLQSRLAGISGLADLEIEKQVLAPQIKVRVDYAKAAQYGVPTPQILS
                     ALQSFVEGEKVAQIVEGGRRFGLVVRVPESARSIEGLSQILIETPNGRVPLSRVATIE
                     DSDGPNQISRDDGKRRIVLSANAQGRPLSEVVDDIRQVVADFRLPEGYFITLGGQFQA
                     QEEASRLVGLLSIVSLVLMFVVLYSRYKSVTLSALIMANIPLALVGAVIGLWLSGQPL
                     SIAALIGFITLAGISVRNGILKVSHYINLMRIEGESFDHAMILRGSLERLSPVLMTAL
                     VTAFALAPLLFEAELPGTEILHPVAVVIFSGLISSTLLDTFLTPAMFWLFARKDTERL
                     MSDRNAEAF"
     gene            complement(3353..4462)
                     /locus_tag="VM406_14840"
     CDS             complement(3353..4462)
                     /locus_tag="VM406_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVL77289.1"
                     /translation="MRAKWIFLVAFIAATALTNPAVAGPGHDHGDEAPAANSNGPTRL
                     PDGSVFLPKPTQRQLGVRTIVAETGELPRAYELAGKVVMDPNAGGKVQPTVAGRLVPG
                     PNGLPSFGQPVRKGEVLAYVIPASGAIERSNQAAQLSELRSAKAVAERRLARLKELSD
                     TVPRKEIEAAESEAQALGKQIAALGKGLSEREALTAPVAGVIASANAAAGQVVEPREL
                     LFEIVDPARLRIEALAYDPEQVTDVAGATVAIGGARVPLEFVGAARLLRQQALPMMFR
                     GENQALSKLAVGQPVRVYVQTASTVKGLRVPAAALMKNPANQTIIWVKAAPERFEPRV
                     VTAEPLDGASVAVTSGLQPGDRVATTGATLINQVR"
     gene            complement(4462..5046)
                     /locus_tag="VM406_14845"
     CDS             complement(4462..5046)
                     /locus_tag="VM406_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77290.1"
                     /translation="MTFSPLTRGRMRPALYAASLVVCVAASFFPFAVQAGAGHDHGEE
                     TPAASGPALPRFAATSEMFELVGVLNGKHLTLYLDHAATNAPVKDARLEIEFGTEKLK
                     VEPHAEGEFEATLAQEPEAGVIPIAATVVAGNDADLLAGELDLHDDHAEEEDHAHDWK
                     EYALWGVGGLLVLVLAALGIRRISRLNNRSGGAA"
     gene            complement(5088..6428)
                     /locus_tag="VM406_14850"
     CDS             complement(5088..6428)
                     /locus_tag="VM406_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HVL77291.1"
                     /translation="MNSLKRRAIARVFKPLNEQSLLLAIAALAWIVPGPILAQAQNRP
                     AEMAPAQVVPTLNEAFSAAWQRQPEAQSLQARREADAAQRDIARSWMAEPPELELSTR
                     TDRINGNQGVREYEAAVAMPLWLPGERQRTAALADAQSRVTSTRLLAAQLRTAAAVRE
                     AYWTWQQALIEQGLAAERLANARVFAADVARRVRAGDLARADQHRAEGAAAAAESAMA
                     ESAAAVAVKAQELRALTGLPVANGVAAKNAAYDKALEAEAEPQLPDDIQALGVDHPLV
                     ASVFAQAEAARRAADLAAAQKRSNPELTVGVTSERGQFGESYEQSLMFGVRIPFGSNS
                     RYQAKVAAAKAEALEAQSQLQLQRDQVLSAVEAARERVAAARAQREAAEKRRRLAADS
                     RGFFEKSFRLGETDLPTRLQIELEAAEAERAAVRARVELASAISGLRQAMGLLP"
     gene            complement(7163..>7817)
                     /locus_tag="VM406_14855"
     CDS             complement(7163..>7817)
                     /locus_tag="VM406_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVL77292.1"
                     /translation="IEKNGDGFTVYASSQGEKIRVDADLVVHAAGRRPDFGPLDLQAG
                     GVAVKDGRLQLNKRLQSTSNPSVYAAGDAARMGPPLTPVSSHDAKVVAKNLLEGNHHS
                     PDYTGVPSVAFTIPPIAAVGLSEAEAQAKGLQYRANSQKASNWFTARQAAEPVYGFKV
                     LVERESDLILGAHLVGPHADEVINLFALAIRHRLTSEDLKTTMFAYPTGASDIGYMV"
BASE COUNT         1459 a         2473 c         2428 g         1457 t
ORIGIN      
        1 gagctgcagc acctgcggag tagcgacgag ttcgtagtct acatccttga cttcggccac
       61 gacaccgccg tgcacaggtt cgtgctcgtg tccgtgctca tgcttgtcgg cggcaaaagc
      121 cgcgttcgat agtacgaggg ctgcggcaaa cataagggct gcaaatttca tgtttcttcc
      181 tttccacgaa tagaaaaatg ggtgagttga tcgttgcatc tcagaaggct tctgcgttcc
      241 ggtcgctcat caggcgctcg gtgtccttgc gtgcgaagag ccagaacatt gcgggcgtga
      301 ggaaggtatc cagcagtgtc gagctgatca gaccggagaa aatcaccact gccaccgggt
      361 gcagaatctc ggtaccgggg agctcggcct cgaacagaag cggggccagc gcaaatgccg
      421 tcaccagcgc cgtcatcagc accggagaca gtcgctcaag cgagccgcgc aggatcatcg
      481 cgtggtcgaa actctccccc tcgatgcgca tcaggttgat gtagtggctg accttgagga
      541 tgccgttgcg tacagagatg ccggccagcg taatgaagcc gatcagggcc gcgatcgaca
      601 gcggctggcc cgacagccac agcccgataa cggcaccgac cagcgcgaga gggatgttcg
      661 ccatgatcag ggccgacagc gtcaccgact tgtagcggct gtaaagcacg acaaacatca
      721 gtaccagcga gacgatggac agcagcccga ccaggcgcga tgcctcttcc tgcgcctgga
      781 actgccctcc gagcgtgata aagtaacctt ccggcagccg gaaatcggcc accacctgcc
      841 ggatatcgtc caccacctcc gacagtggcc tgccctgtgc atttgcagaa agcacgatac
      901 gtcgcttgcc gtcgtcgcgg ctgatctggt tcggtccgtc gctgtcctcg atcgtggcga
      961 cccgcgaaag cggcacccgt ccgttcgggg tttcaatcag gatctgcgat agcccttcga
     1021 ttgagcgagc ggactctggc acacgcacaa ccagcccaaa gcgtcggccg ccttccacga
     1081 tctgcgccac cttctcgcct tcgacgaagc tttgcagggc cgagaggatc tgtggtgtcg
     1141 gcacgccgta ctgcgcggcc ttcgcgtagt ccacacgcac cttgatctgc ggcgcaagca
     1201 cctgcttctc gatctccagg tccgcaagtc cggaaattcc agcgagtcgg gactggagta
     1261 gatccgcttg tccacgcagc gtgtcgaggt cttcgccgaa gatcttgatg gcgatttgcg
     1321 agcgcacgcc cgaaagcatg tggtcgatgc ggtgcgagat cggctgacca atggcgatgg
     1381 ccgcaggcaa gtgggcaatg cgcgagcgga tatcggcctg gatctcgtcc atcgaccgtt
     1441 ccagctcgtc agccggcttc aggccgacgt ccagttcact tacgtgtacg ccttccgcgt
     1501 gctcatcgag ttcggcacga ccgctacgac gcccaacgtg agtgacttct ggcacttggc
     1561 taaccagcac ttccgcctgg cgggccaacg cagaggattc ggccagcgtc accccgggat
     1621 tcagtcgcag tccgatcagc aaggtgcctt cattgaatgg cggcaggaat gaggtcggaa
     1681 agaacggaac cgcggctgcg gcgatcagga ctgcggcggc acccgtgcca acggccgcct
     1741 ttgggtgatt cagtaccgat tgcagcccgc ttcgataccg ctttttcagc cacgccagca
     1801 gcctggtgtc cccatggtcc agcgacttca ttccaggtag caggtagtaa gccagaaccg
     1861 gcgtgacggt caccgacaca accagggagg cgagagtcga gacgatgaag gcgacaccga
     1921 gcgggacaaa cagacgtcct tccatgccgg gcaacgcaaa cagcggcagg aacaccagga
     1981 cgatgatgat tgtggcgtac aggatcgctg aacgcacttc catcgtcgca tgggccacca
     2041 gctgaagcgg accgagccgg tgctccgggt gcttgtcccg gtcgactttc aggcgtcgca
     2101 tcacgttttc gaccccgacc accgcgtcgt cgaccaagcc gccgattgca atcgccagac
     2161 cgccgagggt catcgtgttg atggacatgc cgaagtactt gaagaccagg gcggtgatga
     2221 agatcgagac cggaatggcg gtgagcgcaa tcagcgtcgg gcgtatggtt ccgaggaaca
     2281 ggtacagaat cgcagcgacg aagaccgatg cacctatcag cttgccttga agagtgttga
     2341 tcgacgactc gatgaagctc gcctgacgga aggtgacgcg cggcgcctcc atccctgggg
     2401 gcaagccctt gttcatttcc tccaacgcgg cttcgatcga ccgggtgagc gaaatcgtat
     2461 ccgcggtcgg ctgcttttga atgccgagga tgacagccgg cttgccctca aagccggcgt
     2521 ctccgcgccg agtcgccgct gcaaaggtca cgtcggcgat ctgtcgcagc agaaccggct
     2581 ggccctcccg ggcggttagc gcgagattct tcaggtcctc cagtcgcgat gtctggccca
     2641 gcatgcggat caggtactcg cggccattca gttccaggaa ccctccggac gtgtttgccg
     2701 agtacccttt cagggccgct tccaggtcat cgtgcgtgat gcccagtgct gccatgcggg
     2761 tcgtgtccgg ctgcacctga aactgccgca cctcgccacc gatcgggatg acctgagcga
     2821 cgccgggaat cgccatcaag cgcggacgca agacccagtc ggcatactct cgcacttcca
     2881 tcagcgagat ttttgcggtg tcgaccggga tggcaacctg catgatttca cccatgatcg
     2941 agctgattgg tcccatgcgg gggacgacgt cggccgcaag cccttcttcc attgaggaca
     3001 ggcgctccga taccatctgc cgtgcccgga agatctcggt cgaccaatcg aaggtcacat
     3061 agatgaacga cagaccagca ctggacacgg agcgcacgga ctccaccccc ggcagtccat
     3121 tcatcgtggt ctccagcggg aaggtgatca gttgctccac ttcctcggcg gccatgccgc
     3181 cagcctcggt catgatggtg accgtcggct tgttcaggtc cgggaacacg tcgaccgggg
     3241 tttgcgacag cgtgaaggcg ccgtaggcca tcagcaccag gctggcaatg attaccagca
     3301 ggcggttgct caggctgtta tcgagtagcc acttgaacat gcgcggctct ccttaacgga
     3361 cctggttaat caaggtcgca ccagtggttg cgacgcgatc acccggctgc aacccggatg
     3421 tgacggcaac cgacgcgcca tcaagcggct cggccgtcac gacgcgcggc tcgaagcgct
     3481 cgggcgcggc tttcacccag atgatggtct ggttggccgg gtttttcatc agcgcggccg
     3541 caggcacgcg caaccctttg acggtgcttg ccgtctggac gtacacccgc accggctgtc
     3601 cgacagccag cttcgacagt gcctgattct ctcctcgaaa catcatcggc agcgcctgct
     3661 ggcgcagcaa gcgcgcggcg ccgacgaact ccagcggcac tcgcgcgcca ccgatggcca
     3721 ccgttgcacc agccacatcg gtcacctgtt cgggatcgta ggcgagcgct tcgatgcgca
     3781 ggcgcgccgg gtccacgatc tcgaacagca gctcgcgcgg ctcgacgacc tggccggcag
     3841 cagcgttggc cgatgcgatg acgcctgcga ctggggcggt tagtgcctcg cgctcggaca
     3901 ggcccttgcc caacgccgcg atctgcttgc ccagcgcctg ggcttcgctc tcggccgcct
     3961 cgatttcctt gcgcggcact gtgtcggata actccttcaa gcgcgccaga cgccgttcgg
     4021 ccacggcttt ggccgagcgc agctcggata gctgcgcggc ttgattggag cgctcgatcg
     4081 caccgctggc cggaatcacg taggccagca cctcgccttt acgcaccggc tgaccaaagc
     4141 taggcaaccc attcggcccg ggcaccagtc gccctgcgac cgtcggctgc actttgccgc
     4201 ccgcattcgg atccatcacc accttgcccg ccagctcata ggcgcgcggc agctcgccag
     4261 tttctgcaac gatcgtacgc actcccagtt gccgctgcgt gggcttcggc aaaaatacgc
     4321 ttccatccgg caggcgcgtc ggaccattgc tgtttgcagc cggcgcctcg tccccgtggt
     4381 cgtgtcctgg tccggcaacg gcgggattgg tcaaggcagt ggcggcaata aacgcaacca
     4441 aaaaaatcca cttcgcgcgc atcatgcggc acctccggaa cggttgttga gacggctgat
     4501 gcgacgtatt cccagcgcgg cgaggacgag cacaagcagg ccgccgacac cccacaacgc
     4561 gtattccttc cagtcgtgcg catggtcctc ttcctcggca tggtcgtcgt gcagatcgag
     4621 ctcgcctgcg agcaggtcgg catcattgcc tgccacgaca gtcgcggcga tcggaatcac
     4681 gccggcctcg ggttcctgag cgagcgtggc ttcaaactca ccttcagcat gaggttcgac
     4741 cttcagcttt tcagtaccga actcgatttc gagccgggca tccttgaccg gggcattggt
     4801 ggctgcgtgg tcaagataga gggtcaagtg cttgccgttg agtacgccaa cgagctcgaa
     4861 catctctgag gtggcggcga accgcggcaa ggccggccct gatgctgccg gcgtttcctc
     4921 gccatggtca tgtccagccc ctgcctggac tgcgaacggg aaaaaagacg cagcgacgca
     4981 gacgaccagc gacgctgcat agagcgccgg ccgcatgcga ccgcgcgtca gtggagaaaa
     5041 agtcatcgga tatatccttg gattcagtag attcatggcg gggcggctca gggcaagagg
     5101 cccatggcct gacgcaggcc ggagatagcc gaagcgagct cgacgcgtgc acgcacagct
     5161 gcacgttcgg cttctgccgc ttcgagttcg atctggagtc gcgtcggcaa gtccgtttcg
     5221 cccagtcgga aggatttttc gaagaagcca cgggaatccg cggcaaggcg ccggcgtttc
     5281 tccgcggcct cgcgttgtgc gcgggcagcg gcaacacgtt cccgtgccgc ctctacggcc
     5341 gacagaacct gatcgcgctg cagttgcaac tggctctgtg cctccaacgc ttcggccttg
     5401 gcagcggcga ccttggcttg gtagcgactg ttcgagccga acgggatgcg cacgccaaac
     5461 atcaaagact gctcatacga ctcgccgaac tgtccacgtt cgctcgtgac gccaaccgtc
     5521 agctccgggt tgcttcgctt ttgcgcagca gccaggtcgg ccgcgcggcg cgcggcttcg
     5581 gcctgcgcaa acacgctcgc caccagcgga tggtcgacgc cgagcgcctg gatgtcgtca
     5641 ggcagctgcg gttcggcttc ggcttcaagc gctttgtcgt aggcggcgtt cttggcagca
     5701 accccattgg ccacaggtag tccggtgagc gcgcgcagct cctgcgcctt cacagccacc
     5761 gccgcggcac tttcggccat ggcgctttca gctgctgccg cggcaccttc ggccctgtgc
     5821 tggtccgcgc gcgccaggtc gccggccctg acgcgccggg caacatcagc ggcgaaaacg
     5881 cgtgcatttg cgagacgttc cgcagcaagc ccctgttcga tcagtgcttg ctgccaggtc
     5941 caatacgctt cccgcacggc tgcggccgta cgcagctggg cggccagtag gcgcgtcgac
     6001 gtcactcggg actgggcgtc agccagtgct gcagtgcgct ggcgttctcc gggcagccaa
     6061 agcggcatcg ccacagcggc ttcgtactcg cgcactccct gatttccgtt gatacggtcg
     6121 gttctggtcg acagctccag ttcaggcggc tcggccatcc aactcctggc aatatctcgc
     6181 tgcgcagcgt ccgcttcccg gcgcgcctgc agcgactgcg cttccggctg acgttgccag
     6241 gcggcgctga atgcttcgtt cagggttgga acaacctgtg cgggcgccat ctcggcgggt
     6301 cggttctgag cctgcgcaag gatcggccca ggcacgatcc aggcaagcgc agcaatagcg
     6361 agcagcagag actgctcgtt gaggggcttg aaaacgcgcg cgattgcacg gcgcttcaag
     6421 ctattcattc ggatcatccg attctccaca gttggtctga atagcgtggg gaatcgacga
     6481 ggcagcgagt aagcgggacc cgctctagag cgagcaaatc cggcccggtg gtggccgcgg
     6541 cctgaccgcg atcaccgagc gtgtcctaca ggctatgtgt gaacagtgtg ccccgccgat
     6601 caggcaaggc ttgcccattt gggtcgttca ggggggtacg tcagggagga agaaatgcgg
     6661 tcccacggcg ggacgaagcg ggaagcggcg gaagtgacaa aggtagaggg accttcgttg
     6721 cttagcacta cgaggttccc gccatggcac gttccgcaat cggggtgagg tgcgctgctt
     6781 tgcttgtcgg gcgttcccgc atcaagatcc ggggcgtcgc ccgcggcatg ctcgtgcggg
     6841 tggtgcccga agtggtttgc ctgaggccct gtttcatgcc cgcagtagct tcccgccgcg
     6901 gcccaggaga gctgcagcgg catcagcacc aacaagaaaa ggatcagata gcgacgcata
     6961 agcgtacgga ttatagaggg aattcggctg gccggacaag gcaaggacaa atcgtgttca
     7021 cctgggacca ttctacgaac tggcaggtgc cgctctgcat aatctcgtcc ttggcaaatc
     7081 agcaccgcgg ggcaagtcaa cacgaattga aggcgaggat gggtgtgaac cagcgagaac
     7141 cccttcgcgg caagccagga cgtcacacca tgtaaccgat atccgaggcc cccgtcggat
     7201 aggcgaacat tgttgtcttc aggtcttctg aagtcaggcg atggcgtatc gccagggcga
     7261 acaggttgat cacttcgtcc gcatgagggc ccaccagatg tgcaccgagg atcaggtcac
     7321 tttccctctc gaccaggacc ttgaagccat acacgggttc ggcagcttgg cgcgcggtga
     7381 accaattcga tgccttttga ctgttcgcgc ggtactggag ccctttcgcc tgagcttcgg
     7441 cctcggacag gccgactgca gcgatcggcg gaatcgtgaa ggctacgctg ggtacacctg
     7501 tgtaatctgg actgtggtga ttgccttcga gcagattttt ggcgacgacc ttggcgtcat
     7561 ggctggatac cggcgtcagc ggcggcccca ttcgggcggc atcaccggct gcgtacaccg
     7621 acggattgga agtgctttgc aggcgcttgt tgagctgaag cctcccatcc ttcaccgcca
     7681 cgcccccggc ttgcagatca agcgggccaa agtcgggccg gcggccggcg gcatgcacga
     7741 ccaggtctgc gtccacgcgg atcttctcgc cttggcttga agcgtagaca gtgaaaccgt
     7801 cgccgttttt ttcgatt
//
LOCUS       DATLGF010000480         5446 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_123065, whole genome shotgun sequence.
ACCESSION   DATLGF010000480 DATLGF010000000
VERSION     DATLGF010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123065"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="VM406_14860"
     CDS             complement(<1..1207)
                     /locus_tag="VM406_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HVL77293.1"
                     /translation="MRKLPLHEMRTLVRFALRRLEEERLPEVAGSLTFTTVLALVPVL
                     TVVLAVFTAFPIFETFRAAVEDYFLQTLVPGTIADTIIENLNQFAAQARTLSAIGAIG
                     LVVTTIVTMLTIDSAFNHIWRVKTPRPLLQRVMVYWTLVTFGPLLAGVSVTVTSDLYA
                     RVGGWTPEVPMVAAALYTLVSVLLTAFALTLLYMAVPNRYVDWRDAAWGGVLAAIAFE
                     AAKTIFVVFITGFPTYAVVYGALAALPIFLLWVYVSWLIALIGAVIAAALPVVKHERW
                     WHVAAPGSTFIDAMAVLKVLVDARAHGSNAAVDAGTLRAATRLGYDELESLLQRMLEV
                     GWVGRMAPLRTRRVQFGKRINEGRDRWVLTHNPDQLTVADVYRLFAFNTMESPALALQ
                     VDEAVSAGLA"
     gene            1283..1891
                     /gene="wrbA"
                     /locus_tag="VM406_14865"
     CDS             1283..1891
                     /gene="wrbA"
                     /locus_tag="VM406_14865"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HVL77294.1"
                     /translation="MISSDLTVLILYYSRHGATRKMAELIAQGVESVPGCEARLRTVP
                     AVSTVAEAIEPEVPPSGAPYVELRDLEECAALALGSPTRFGNMAAPLKYFIDGTATQW
                     LSGALAGKPACVFTSTGSLHGGQETTLLSMMLPLLHHGMLLLGLPYTDARLMTSASGG
                     TPYGASHWAGAGGNLSLTEETRALTIALGQRLAQTAVKLNRC"
     gene            1901..2281
                     /locus_tag="VM406_14870"
     CDS             1901..2281
                     /locus_tag="VM406_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HVL77295.1"
                     /translation="MQKTYYYSACASLVALIILCIAWEMWLAPLRPGGSWLVLKVIPL
                     LLPLRGVFKRDLYTMQWASMLILLYLAEGLVRATSDPPASAWLGWIEVALVTIFFFST
                     ILYLRPYKKAAKALARRAIEKASE"
     gene            2278..3684
                     /locus_tag="VM406_14875"
     CDS             2278..3684
                     /locus_tag="VM406_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HVL77296.1"
                     /translation="MTDFLEQCRAITGAANVLTEPADMAGYLTDWRRRFTGKARAVLR
                     PGSTDEVAALVRLCASLGIPLVPQGGNTGLVLGSVPDTSGEAVVLSLTRLNRIRAVDT
                     TNNTITAEAGVVLKQLQEAAAESGRLFPLSLAAEGSCTIGGNLATNAGGTAVLRYGNM
                     RELCLGLEVVTAQGEIWNGLRGLRKDNTGYDLRDLFIGAEGTLGIITAATLKLYPRPR
                     AQLTALAATDSPENALRLLNLAQDRCGPALTGFELMSDVCLKLVEKHYPQMRLPFDAR
                     HAQYVLLEISDSESEEHANAMLEDLIGQALEQETIADAVVASSIAQSRALWNLREHIP
                     LAQADEGKNIKHDISVAISHIAAFIRTTDALLQQAYPGCRMVTFGHLGDGNLHYNVSP
                     PEGQSPDAFVAHQEAINRIVHDSVQRFGGSISAEHGVGALKRDEIRVYKSELEIRMMA
                     AVKRALDPQNLMNPGKIV"
     gene            complement(3771..3847)
                     /locus_tag="VM406_14880"
     tRNA            complement(3771..3847)
                     /locus_tag="VM406_14880"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3811..3813),aa:Arg,seq:tct)
     gene            complement(3906..3982)
                     /locus_tag="VM406_14885"
     tRNA            complement(3906..3982)
                     /locus_tag="VM406_14885"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3946..3948),aa:Pro,seq:tgg)
     gene            complement(4041..5375)
                     /gene="glmM"
                     /locus_tag="VM406_14890"
     CDS             complement(4041..5375)
                     /gene="glmM"
                     /locus_tag="VM406_14890"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HVL77297.1"
                     /translation="MTRKYFGTDGVRGRVGTAPITPDFVMKLGYAAGKVLAQAEATSA
                     HPVVLIGKDTRVSGYMLEAALEAGFSAAGVDVMLAGPMPTPAIAYLTRALRLSAGVVI
                     SASHNPYDDNGIKFFSASGNKLPDAVEAAIEAALEEPMSCAPSEHLGKAKRLDDARGR
                     YIEFCKSTFPNELDLRGLKIVVDCAHGAAYHIAPDVFHELGAEVVAIGNRPNGFNIND
                     GVGATAPATLCAAVKEHGADLGIALDGDADRLLMVDSTGRAYNGDELLYVMARDRLKK
                     GPVAGVVGTLMTNMALEVALRRMDVPFARAKVGDRYVLEVLQERGWLIGGEGSGHLLC
                     LDKHTTGDGIVSALQVLCALKRSAQTLEQATADLVLFPQTLINVRVAPGTNWQLNAAL
                     MTDKERAEAELGDDGRVLIRASGTEPLVRVMVEARDAEIARKTAERLAAHLA"
BASE COUNT         1012 a         1829 c         1708 g          897 t
ORIGIN      
        1 ctgcgaggcc ggcagacacg gcctcgtcga cctgcagcgc aagcgccgga ctctccatcg
       61 tgttgaaggc gaacagacgg tagacgtcgg caacggtcaa ttgatcggga ttgtgcgtca
      121 gcacccagcg gtcgcgcccc tcgttgatgc gcttgccgaa ctgcacgcgg cgcgttcgca
      181 gcggcgccat gcgcccgacc caacccacct ccagcatacg ctgcagcagc gactccaact
      241 cgtcgtagcc cagacgcgtg gcggcgcgaa gcgttcccgc gtcgacagcc gcattgctgc
      301 cgtgcgcgcg tgcatcgacc aggaccttca gaaccgccat cgcgtcgatg aaggtgcttc
      361 ccggcgcagc gacatgccac cagcgctcgt gcttgaccac cggcaaggca gcagcgatca
      421 ccgcaccgat caacgcgatc agccaggaga catacaccca caacaggaag attggaagcg
      481 cggccagcgc gccgtagacg accgcatagg tcgggaatcc agtgatgaac acgacgaata
      541 ttgtcttggc cgcctcgaac gcgatggccg cgagcacccc gccccatgcg gcatcacgcc
      601 agtcgacgta acggttcggt accgccatgt ataaaagggt cagcgcgaac gccgtcagca
      661 agaccgagac caaggtgtac agtgccgccg ccaccatcgg cacctcgggc gtccatccgc
      721 ccactcgcgc gtagagatcg gaggtcacgg tcaccgacac gccggccagc agcggaccga
      781 acgtgaccag cgtccagtac accatcaccc gctgcagcag cgggcgtggc gtcttcaccc
      841 gccagatgtg attgaaggcg ctgtcgatcg tgagcatggt cacgatcgtc gtcacgacca
      901 ggccgattgc gccgatcgcc gacagcgtgc gcgcctgagc cgcgaactgg ttcaggttct
      961 cgatgatggt atcggcaatc gttccaggca ccagggtctg gaggaagtag tcttcgacag
     1021 cggcgcggaa ggtttcgaaa atcgggaacg ccgtaaagac tgcgagtacc acggtcaata
     1081 ccggtaccag cgcgagcact gtggtgaatg tgaggctgcc ggccacttcg ggaaggcgct
     1141 cctcttccag tcggcgcagt gcgaaacgca ccagcgtgcg catctcgtgc agagggagct
     1201 tgcgcatcag gttcgttatc aatcgcatcc ggtgcagtcg ggcggcgggg ccagcgtgag
     1261 cggaacacct ataataccaa gcatgatttc cagtgacctg acagtactca tcctgtacta
     1321 ctcgcgccat ggcgccaccc gcaagatggc cgaactgatt gcccagggcg tcgaaagcgt
     1381 gcccggctgc gaagcgcgcc tgcgcacggt gcccgcggta tccaccgtgg ccgaagccat
     1441 cgagcccgag gtgccgccca gcggagcgcc gtacgtggag ttgcgcgatc tggaggaatg
     1501 cgcggccctg gcgctcggct cgcctacccg ctttggcaac atggcggcgc cgttgaaata
     1561 tttcatcgac gggacagcga cgcaatggct gtccggtgca ttggccggca agcccgcctg
     1621 cgtgttcacc tcgaccggca gcctgcacgg cggacaggaa acaaccctgc tttcgatgat
     1681 gctgccgctg ctgcaccacg ggatgctgtt gctcggcctc ccctataccg atgcgcgcct
     1741 gatgaccagc gccagcggcg gcacgcccta cggcgccagc cactgggccg gcgcgggcgg
     1801 caatctgtcg ctcaccgagg aaaccagggc actcaccatc gcactcggcc aacgcctggc
     1861 gcaaaccgcg gtcaaactca accgctgctg ataccggaac atgcaaaaga cttactacta
     1921 cagcgcctgc gccagcctgg tggccctcat catcctgtgc attgcatggg agatgtggct
     1981 cgctccgttg cggccgggcg gctcctggct ggtcctgaag gtgatcccgc tgctgctgcc
     2041 gctacgcggc gtgttcaagc gcgacctgta tacgatgcag tgggcatcga tgctgatcct
     2101 gctgtacctg gccgaggggc tggtgcgcgc gaccagcgat ccgccggcct ctgcgtggct
     2161 gggatggatc gaagtcgcgc tggtcaccat cttcttcttc agcacgattc tctacctgcg
     2221 cccctacaag aaagcggcca aggcgctcgc gcgacgggca atcgaaaagg catcggaatg
     2281 actgattttc tcgagcagtg ccgcgccatc accggcgcag ccaacgttct gaccgaaccg
     2341 gccgacatgg ccggatacct gaccgactgg cgccggcgct tcacgggaaa ggcgcgtgcc
     2401 gtgctgcgcc ccggctccac ggatgaagtg gcggcactcg tgcggctctg cgcaagcctc
     2461 ggcattcccc tggttccgca gggcggcaat accgggctcg tacttggcag cgtacccgat
     2521 acgagcggcg aagcggtggt gctgtcactc acccggctaa accgcatccg cgcggtcgac
     2581 accacgaaca acacgatcac cgccgaagcc ggcgtggtcc tgaagcagct gcaggaggcg
     2641 gcggcagaat ccggccggct gtttccgctc tcgctggccg ccgagggaag ctgcacgata
     2701 ggcggcaatc tcgctaccaa tgcgggcggc accgcggttc tgcgctacgg caacatgcgc
     2761 gagctgtgcc tcggtctgga agtggtgacc gcacagggcg aaatctggaa cgggttgcgc
     2821 gggctgcgca aggacaatac cggctacgac ctgcgcgatc tgttcatagg cgcggaaggc
     2881 acgctgggca tcatcaccgc ggccacgctg aagctctatc ctcggccgcg cgctcagctc
     2941 accgcgctgg ccgcgaccga ctcgccggag aacgcattgc gcctgctgaa cctcgcgcag
     3001 gaccgctgcg ggccggcgct caccggcttc gaactgatgt ccgatgtctg cctgaagctg
     3061 gtcgagaagc actacccgca gatgcggctg cctttcgatg cacgccatgc gcaatacgtg
     3121 ttgctcgaaa tctccgacag cgagtccgag gagcatgcga acgcgatgct ggaggacctg
     3181 atcgggcagg cgctggagca ggaaacgatt gccgacgcgg tggtcgcctc gtccatcgcg
     3241 cagtcgcgcg cgttgtggaa tctgcgcgag cacattccgc tcgcgcaggc cgacgaaggc
     3301 aagaacatca agcacgacat ctcggtcgcg atctcgcaca tcgccgcatt catccgcacc
     3361 accgacgccc tgctccagca agcctatccc ggctgccgca tggtgacctt cggccacctg
     3421 ggcgacggca acctgcatta caacgtgtcg ccgccagagg ggcagtcgcc cgacgcgttc
     3481 gtcgcgcacc aggaagcgat caaccgtatc gtgcatgaca gcgtacagcg cttcggcggc
     3541 tcgatctccg ccgagcatgg cgtgggcgca ctcaagcgcg acgagatccg cgtctacaag
     3601 tcggaactgg aaatcaggat gatggccgcg gtgaagcgtg cgctcgatcc gcagaatttg
     3661 atgaatccgg gaaagatcgt ctgagccgcc gggcgtgcgg gccgcggcaa ccggagggag
     3721 cggcggccgg gtctcccatg ccaatccggc ttggacaggg acggaaagaa tggcctgccc
     3781 agcaggagtc gaacctgcga cctacggctt agaaggccgt tgctctatcc agctgagcta
     3841 tgggcagtcg gggaacgacc ggacgaatgg ccgccttgcg gcggccgccc aattggcggg
     3901 aattctggtc ggggcgagac gattcgaacg tccgaccacc tgatcccaaa tcaggtgcgc
     3961 taccaggctg cgctacgccc cgagaaccaa gcatcatacc gggatgcacc atcccggtca
     4021 aacttgctgc accacactgt tcaggcgagg tgtgcggcga ggcgctccgc ggtctttcgg
     4081 gcgatctccg cgtcgcgcgc ctccaccatc acgcgcacca gcggctcggt gccggatgcg
     4141 cggatcagta cgcggccgtc gtcgccgagc tccgcctcgg cacgctcctt atcggtcatc
     4201 aacgcggcat tcagctgcca gttcgtcccc ggtgcaacgc gcacattgat cagcgtttgc
     4261 gggaacagca cgagatcggc cgttgcctgc tccagggtct gtgcgctgcg cttgagcgca
     4321 cacagcacct gcaacgcgga gacgatgccg tcgccggtcg tgtgcttgtc caggcacagc
     4381 aggtggcccg acccttcgcc gccgatcagc cagccgcgct cctgcagcac ctcgagcacg
     4441 tagcggtcgc ccaccttggc gcgcgcgaat ggaacgtcca ttcgccgcag cgccacttcc
     4501 agcgccatgt tggtcatcag cgtgccgacc acgccggcga ccggcccctt cttgagcctg
     4561 tcccttgcca tcacgtacag cagttcgtcg ccgttataag cccggccggt cgagtcgacc
     4621 atcagcaggc ggtcggcatc gccgtccagc gcaataccca ggtcggcgcc atgttccttc
     4681 accgccgcgc acaaggtcgc cggcgcggtc gcccctacgc cgtcgttgat gttgaagccg
     4741 ttcgggcggt tgccgatcgc gacgacctcg gcacccagct catggaaaac gtcaggcgca
     4801 atgtgatagg ccgcgccgtg cgcgcaatcg acgacgatct tcaggccacg aaggtcgagc
     4861 tcgttcggaa acgtgctctt gcagaattcg atatagcgac cccgcgcgtc atcgagccgc
     4921 ttggctttgc ccagatgctc cgacggcgca cacgacatcg gctcttccag cgcggcttcg
     4981 attgccgcct cgaccgcatc cggcagcttg ttgccggagg ccgagaagaa tttgatgcca
     5041 ttgtcgtcat acgggttgtg ggacgccgaa atcaccacac cggccgacag ccgcagcgcg
     5101 cgcgtgagat aagcaatggc cggcgtcggc atcgggcctg ccagcatcac atccactcct
     5161 gcggcggaga aacccgcttc cagggcagcc tcgagcatgt aaccggacac acgcgtgtcc
     5221 ttgccgatca gcacgaccgg atgggcactc gtggcttcgg cctgtgccag caccttgccg
     5281 gcggcatacc cgagcttcat cacgaaatcc ggggtgatcg gagcggtgcc gacgcggccg
     5341 cggacaccgt cggtgccgaa atatttacga gtcatgttct tccttggtta atcgcgttgc
     5401 gtggcagcct gccacacctt gagggcgtcg acggtttcgg caacgt
//
LOCUS       DATLGF010000481        10932 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_217979, whole genome shotgun sequence.
ACCESSION   DATLGF010000481 DATLGF010000000
VERSION     DATLGF010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 10932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10932
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217979"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1990
                     /locus_tag="VM406_14895"
     CDS             <1..1990
                     /locus_tag="VM406_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HVL77298.1"
                     /translation="GIIFDALYRRHVRLPFAMRERLEVVLGPNHVARLDETGPAGLRA
                     LAGAVNRLAEQRDALERDVAARVDEAKWRIEEERNRFATLIAELSQSIIVCNADGRIL
                     LYNNRARALFSSETDGGNIGAALVGLGRSIFGVLEHSLIAHGLEKIQQRLACGEARPI
                     ANFVTATPGGQLVRAQMAPVLQRERETADETDETDATIGGYVLILDDITRRVDLESQR
                     DQLLQSLTEGARAALGNIRAAIENLSEFPDMEAPQRDRFIGIVREEAQAMSARLDRTV
                     SQYSDALKVRWPLEEMAGTDLIQAAQRRVTQRTGLPAKTEELESGLWLKVDSFSLLQG
                     ISYLVARLQDEFNIREIRLRLLRSGRHAHVDIIWSGTVVSTETLAGWDLDPMSAAGES
                     SPLTLREVIDRHDGELWFQREKLRHRAYFRILLPAAGAPPVPEPAPAAYGSRPEYYDF
                     DLFQRTEASDALDQLPLAQLAYTVFDTETTGLEPSAGDEIIQIGAARIVNGRLLRGEV
                     FEQLIDPRRPITAASVRVHGITQDMVVGQPTIERVLPRFHAFCQDTVLVAHNAAFDMR
                     FLQLKENATGVAFRQPVLDTLLLSAVVHPNQDLHSLESIAERLGVALVGRHTALGDAI
                     VTGEVLLKLIPLLAAQGIHTLRQAREASARTYYAKVGY"
     gene            1992..3449
                     /locus_tag="VM406_14900"
     CDS             1992..3449
                     /locus_tag="VM406_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HVL77299.1"
                     /translation="MAEAQDAPAPQASRTMYSTLGSIARGDPVWVGPQASVREAMQLM
                     EQHKVGSVIIADPATGAPLGIFTLQDVLRRISLPAADSGQSISSVMTPDPVSLGREAT
                     AYQAALLMARRKVRHVLVVDDAGRLAGIVSQNDLYALQRVGIKDVSAEIAGAGDMDSL
                     VHASGEIRKLAHSMLAQGVGAEQLTHFISTLNDLLTLRIIELTLAEFDLGGIDWCWIA
                     LGSEGRFEQTFSTDQDNGIIFACAKGEADALRARLLPFAQAVNRKLDACGFLLCKGNI
                     MAGNPMWCLSSDEWRNTFAGWIRAAEPEALLFASIFFDFRPLYGNDELSRSLRGWVLE
                     ACASQPIFLRHMAANALQCQPPLGLIREFVFDRPREFPHTIDLKMYGAWPFVDSARIL
                     ALAHGVAHTSTAQRLRAVMEKIAYSADDAAAIVDSFFYIQLLRLRHQHRLRGQSTGAN
                     RIDPDDLNEFERQILKQAFKQARKLQTRLKLDYQL"
     gene            complement(3473..3775)
                     /locus_tag="VM406_14905"
     CDS             complement(3473..3775)
                     /locus_tag="VM406_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77300.1"
                     /translation="MNPPELFLISVARVLVEVALFTLVGQAVLAIFAGKSRERNVVYR
                     LMQTITGPAVKTVRFVTPRFIIDAHIPFVTFFLLFWIWIALAAFKRYVCVTQGLVC"
     gene            3926..4516
                     /locus_tag="VM406_14910"
     CDS             3926..4516
                     /locus_tag="VM406_14910"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVL77301.1"
                     /translation="MNFEKHRHFLRARLFETFKRQDRALDDYRLALQHDPQFFKAAVA
                     IALIHVQREDYAQAEIFYREALRIKPGHADIIFNLGYVHDQQGRREEAISCFREAVQL
                     KPTLDRAWYGMGMAYAALGRHDEAANALEEASRLQPMNTYAWYALGMAHHRLNNPERV
                     KEVALYLHRIGPLSCRQLVQDTQRSDLQYLIKDMAV"
     gene            complement(4859..6946)
                     /locus_tag="VM406_14915"
     CDS             complement(4859..6946)
                     /locus_tag="VM406_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HVL77302.1"
                     /translation="MATSQSAFNQQLKKYYTFYTGGFFTFIVLLALAEFAGMPARWIG
                     YIFLFATIGLYAGIGIMSRTADVSEYYVAGRRVPAVFNGMATGADWMSAASFIGMAGT
                     LYATGYDGLAFIMGWTGGYCLVALFLAPYLRKFGQFTIPDFLGARYGGNLPRLIGVLA
                     AIIVSFTYVVAQIYGVGIITSRFTGLEFGIGVFVGLAGILVCSFLGGMRAVTWTQVAQ
                     YIILIVAYMIPPVWLAAKQTGVPIPQVVYGVQLEKVSAREKVLNDASTPDGAKEAEVR
                     AILKGRADAANEKLKNPEAAFAADRAALEQKVAELTGANAPADQVDAANAALANYPKD
                     VEAAKAMWNRDKGLAARAGPIKAHAEPFAGPTEEARNKARLNFLTLVFCLMVGTAALP
                     HILMRFYTTPSVKEARRSVFFSLFFIFLLYFTAPALAVLVKFDIYHNLVGSSFASLPA
                     WVGAWAKVDPTLMNIADLNGDGIVQLAEIVIGGDLIVLATPEIAGLPYVISGMVAAGG
                     LAAALSTADGLLLTIANALSHDVYYKVIDPNATTTRRIAIAKTLLLVVALLAAYVTSL
                     KPGDILFLVGAAFSLAASAFFPALVCGVFWRRANKTGAVVGMLAGLGICMYYMIQTYP
                     FFGVNAPQWFGIAPISAGIFGIPVGFAGVIIGSLLTPAPAREIQELVTTVRYPNLDGD
                     GAATAPSGVTKPA"
     gene            complement(6949..7212)
                     /locus_tag="VM406_14920"
     CDS             complement(6949..7212)
                     /locus_tag="VM406_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HVL77303.1"
                     /translation="MQLSAKHEEYWHRNLKITGVLLAIWFFVTFFFGFFARELSSITL
                     LGFPLPFYMAAQGSLIVYVLIIWYYARYMNDLDNEYGVHEGED"
     gene            complement(7485..8798)
                     /gene="gltA"
                     /locus_tag="VM406_14925"
     CDS             complement(7485..8798)
                     /gene="gltA"
                     /locus_tag="VM406_14925"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HVL77304.1"
                     /translation="MTNSDTKATLSFSDGSPSLDLPIYKGTIGPDVIDIRKLYGATGK
                     FTYDPGFMSTAACNSSITYIDGDKGELLYRGYPIEQLAVNCDFLETCYLLLNGELPTA
                     AQKQKFVSTVTNHTMVHEQMQFFFRGFRRDAHPMSVLVGTVGALSSFYHDSLDINDPH
                     HREVSAIRLIAKLPTLVAMSYKYSIGQPFIYPRNDLSYSANFMRMMFATPCEDYKVND
                     VLVRALDRILILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGPAHGGANEAA
                     LQMLEDIQQQGGVERIGEFIAKVKDKSSNVKLMGFGHRVYKNYDPRAKLMRETCHEVL
                     NELGLHDDPLFKLAMALEKIALEDDYFVQRKLYPNVDFYSGIVQRALGIPVSLFTGIF
                     AMARTVGWIAQWNEMISDPEQKIGRPRQLFVGSPRRDVPAIDKRA"
     gene            complement(8885..9154)
                     /locus_tag="VM406_14930"
     CDS             complement(8885..9154)
                     /locus_tag="VM406_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HVL77305.1"
                     /translation="MSITHQADPVKRARLRWRARRGLLENDLIITRFLDAHETALTDE
                     EVDALSRLLELADNELMDLLLARKEPEGEVDQPHVRALLARLRAA"
     gene            complement(9238..9948)
                     /locus_tag="VM406_14935"
     CDS             complement(9238..9948)
                     /locus_tag="VM406_14935"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HVL77306.1"
                     /translation="MPRTLKFKIYRYDPDKDAKPYMQDLTVELQDTDKMLLDALQRIK
                     SDVDDSLALRRSCREGVCGSDAMNINGKNGLACTTNLNELSEPIVLRPLPGLPVIRDL
                     IVDMTQFFKQYHSIKPYLINDSIPPEKERLQTPAEREELDGVYECILCACCSTSCPSF
                     WWNPDKFVGPAGLLQAYRFIADSRDEATNERLDNLEDPYRLFRCHSIMNCVDVCPKGL
                     NPTRAIGKIKELMVRRAV"
     gene            complement(9960..>10932)
                     /locus_tag="VM406_14940"
     CDS             complement(9960..>10932)
                     /locus_tag="VM406_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HVL77307.1"
                     /translation="LLNSNGERFMERYAPTLKDLAPRDFVSRSMDQEIKEGRGCGPNK
                     DHVLLDLRHIGADTIKKRLPSILEIGHKFANVDATKEPIPVVPTIHYQMGGIPTNIHG
                     QVVVPKNGAQEVVNGMYAIGECACVSVHGANRLGTNSLLDLVVFGRAAGNHIVAQNLK
                     AKSHKPLPADAGDVALERLARLESSTGSERVQDVANAIRSTMQQYCGVFRTDELLQAG
                     VQKIMELDERRKHVAFKDKSKVFNTARVEALELDNLIETAKATIVSAAARKESRGAHA
                     HRDYEKRDDENWMKHTLWFSDGNRLDYKPVNTKPLTVETFKPKARTF"
BASE COUNT         2024 a         3426 c         3489 g         1993 t
ORIGIN      
        1 ggggataatc ttcgacgcgc tctaccgtcg ccatgtgcgc ctgccgtttg cgatgcgcga
       61 gcggctggaa gtcgtgctcg gccccaatca tgtcgcgcgg ctggatgaaa cgggcccggc
      121 cggcctgcgc gcgctggcag gcgcggtcaa tcgcctggcc gagcagcgcg acgcgctcga
      181 acgcgacgtc gcggccagag tcgacgaggc gaaatggcgc atcgaggaag aacgcaaccg
      241 attcgccacc ctgattgccg agctcagcca aagcatcatc gtctgcaacg cggatggacg
      301 catcctgctc tacaacaacc gtgcgcgtgc gctgttctcg tcggagaccg acggcggcaa
      361 catcggggcg gcgctggtcg ggctcgggcg ctcgattttc ggcgtgctcg aacacagcct
      421 gatcgcgcac gggctggaga agatccagca gcgcctggcg tgcggggaag cgcggccgat
      481 tgccaatttt gtcaccgcca cccctggcgg ccagctggtg cgcgcccaga tggcgccggt
      541 gcttcagcgg gagcgcgaga ccgcggacga aacggatgag accgacgcga cgatcggtgg
      601 ctacgtactg atcctcgacg acatcacccg gcgcgtcgat ctggaaagcc agcgggacca
      661 actgctgcaa tcgctgaccg agggcgctcg cgcggccttg gggaatatcc gcgccgcgat
      721 cgagaacctg tcggagtttc ccgacatgga agcgccccag cgcgaccgct tcatcggtat
      781 cgtgcgcgaa gaagcgcagg ccatgagcgc aaggctggac cgtaccgtca gccagtactc
      841 ggacgctttg aaagtgcgct ggccgctcga ggaaatggct ggtacggacc tgatccaggc
      901 ggcccagcgc cgggtgacgc agcgcaccgg gctgcccgcc aagacagaag aactggaaag
      961 cggcctctgg ctgaaggtcg acagtttctc gctgctgcaa ggcatcagtt atctcgtcgc
     1021 ccgcctgcag gacgaattca atatccggga aatccgcttg cgcctgctgc gcagcgggag
     1081 gcacgcccat gtggacatca tctggtccgg caccgtggtc tccaccgaaa cccttgccgg
     1141 atgggacctc gacccgatga gcgccgccgg cgagtcctcg ccgctgacgc tgcgcgaagt
     1201 gatcgaccgt cacgacggcg agctgtggtt ccagcgcgag aagctcagac accgcgccta
     1261 tttccgcatc ctgcttccgg ccgccggcgc gccgcccgtg ccggagcctg cccccgcagc
     1321 ctacgggagc cgtcccgagt actacgattt cgacctgttc cagcgcaccg aggcatccga
     1381 cgcgctggat cagctgccgc tcgcgcagct cgcctacacc gtgttcgaca ccgagaccac
     1441 cggcctggag ccctcggcgg gtgatgaaat catccagatc ggcgccgccc gcattgtgaa
     1501 cgggcgcctg ctcaggggcg aggtgttcga gcaattgatc gatccgcgcc gcccgatcac
     1561 cgccgcctcc gtccgcgtgc acggcatcac ccaggacatg gtggtcggcc agccgaccat
     1621 agaacgcgta ctgccgcgct ttcatgcgtt ctgccaggac acggtgctgg tcgcccacaa
     1681 cgccgcattc gacatgcgct tcctgcagct gaaggagaac gcgaccggcg tggcgtttcg
     1741 ccagcctgtg ctcgataccc tgctgctttc cgccgttgtg catccgaacc aggatctgca
     1801 cagtctcgaa tcgatcgcgg aacgcctggg cgtggctctc gtgggccgcc acaccgcgct
     1861 cggcgatgcg atcgtgaccg gcgaggtcct gctgaagctg attccgctgc tcgccgccca
     1921 gggcatacat accttgcgcc aggcgcgcga ggcgtcggca cgcacctatt acgcgaaggt
     1981 ggggtactga aatggccgaa gcacaggacg caccggcgcc gcaagcgagc cgcacgatgt
     2041 acagcacgct gggcagcatc gcccgcggcg atcccgtctg ggtcggtccg caagcgagcg
     2101 tacgggaggc gatgcagctg atggaacagc acaaggtcgg ctcagtcatc attgccgatc
     2161 ccgccacagg tgcgccgctt ggcatcttca cgctgcagga cgtgcttcgt cgcatctccc
     2221 tgcctgcggc cgactccggg caatctattt cgagtgtgat gacgcccgat ccggtctcgc
     2281 taggccgcga ggcgaccgcc tatcaggcgg cgctgctgat ggcgcggcgc aaggtccggc
     2341 atgtgctcgt ggtggacgac gccgggcgcc ttgccggcat cgtgtcgcag aacgatctct
     2401 atgccctcca gcgcgtgggc atcaaggacg tcagcgccga gatcgccggc gccggcgaca
     2461 tggactcgct ggtgcatgca tcgggcgaaa tacgcaagct tgcacacagc atgctcgcgc
     2521 aaggcgtcgg agccgagcag cttacgcact tcatctcgac gctgaacgac ctgctgacct
     2581 tgcgcatcat cgagctcacg ctggccgaat tcgacctcgg cggcatcgac tggtgctgga
     2641 tcgcgctcgg ttcggaaggc cgcttcgagc agacgttcag cacggatcag gacaacggca
     2701 tcatcttcgc ctgcgccaaa ggcgaagcgg atgcgctgcg cgcgcgactg cttccatttg
     2761 cgcaggccgt gaaccgcaag ctggacgcct gcggcttcct gctctgcaag ggcaatatca
     2821 tggccggcaa tccgatgtgg tgtctgtcgt cggatgaatg gcgcaacaca ttcgcaggct
     2881 ggatacgtgc ggccgagccc gaggccttgc tgttcgcctc gatctttttt gatttccgac
     2941 cgttgtacgg aaacgacgag ctgagccgca gcctgcgcgg ctgggtgctg gaagcctgcg
     3001 caagccagcc aatcttcctg cgccacatgg cagccaatgc actgcaatgc cagcctccgc
     3061 tggggctgat ccgggagttc gtgttcgacc ggccgcgcga gttccctcac acgatcgatc
     3121 tgaagatgta cggagcgtgg ccattcgtcg acagtgcccg catcctggcg cttgcgcatg
     3181 gcgtcgcgca taccagtacc gcgcaacggc tcagggcggt gatggagaaa attgcctact
     3241 cggccgacga tgcggccgcc attgtggaca gcttcttcta catacagctg ttgcgcctgc
     3301 gccaccagca caggctgcgc ggccagtcga cgggcgcgaa ccgcatcgac cccgacgacc
     3361 tcaatgaatt cgagcggcag atcctgaagc aggcgttcaa gcaggcgcgc aagctgcaga
     3421 cgcgcctgaa gctcgactat cagctataga tagggccggt gcctgtcccg gcttagcaga
     3481 ccaggccctg cgtaacgcag acatagcgct tgaatgccgc aagcgcgatc cagatccaga
     3541 acaacaggaa gaaggtcacg aacggaatgt gcgcatcgat gatgaagcga ggcgtgacga
     3601 agcgcacggt cttgacggcg gggccggtga tcgtctgcat caggcgatag acgacgttgc
     3661 gctcgcgcga cttgcccgcg aagatggcca gaaccgcctg cccaaccaac gtaaacaggg
     3721 cgacctcgac aagcacacgc gctacgctga tcagaaacag ttcgggagga ttcatcgggt
     3781 gtctcactct ggctcttggc cttgttatgt gtgaatgtat tctagccgag cattgccgct
     3841 gtggcgcatc gaaaatcgac ttgagcgcag gcgcgcagcc aagccataat gcgttcatcg
     3901 atttccaggc caaggaaagg ctgcaatgaa cttcgagaag caccgccact ttctccgtgc
     3961 ccgcttgttc gagacattca agcgacagga ccgggcgctc gacgactacc gcctcgcgct
     4021 gcagcatgac ccgcagttct tcaaggcggc tgtggcgatt gcgctgatcc acgtgcagcg
     4081 ggaggactat gcgcaggccg agattttcta tcgcgaagcg ctgcgcatca agccgggcca
     4141 cgccgacatc atcttcaacc tcggctacgt ccatgaccag cagggccggc gcgaggaggc
     4201 gattagctgc ttccgcgaag ccgtgcagct caagccgacg ctggatcggg cgtggtacgg
     4261 catgggcatg gcctacgccg cgctgggccg ccatgacgag gcggccaacg cgctcgagga
     4321 agcgtcgcgc ctgcaaccga tgaacaccta tgcgtggtac gcgctcggca tggcccacca
     4381 ccgcctgaac aatccggagc gcgtaaagga agtcgctctc tacctgcatc gcatcgggcc
     4441 gttgagctgc cgccagcttg tgcaggatac gcagcgcagc gatcttcagt acctgatcaa
     4501 ggacatggcg gtgtagtccg tcgggggttc cttgacggac tacaccgcga cggcgcacgc
     4561 accacttctt gtcgccccgg cgaaagccgg ggtccagcgt cgctcactac ctccagagtc
     4621 ttgttgcttc acttggatac gttccctcgg ccgtcacggc aagtgccaga gcaccctgta
     4681 cagaaagtcg ccggatccgg acttccgtcg ggatgacatc aggtagccgc tgcattccgg
     4741 ccctcgtcgc gaggcggccg atgtgccgcg acggccggtt gaaggctctt cacttttcga
     4801 cacctccatg aaaaaaggcc cgcttgcgcg ggccttttta ctactgctca agctgacatc
     4861 aggccggctt ggtgactccg ctcggggcgg tagcagcacc gtccccatcc aggttcggat
     4921 agcgcacggt ggtcaccagc tcctggatct cgcgcgccgg tgccggcgtc agcaggctgc
     4981 cgatgatcac gccggcgaag ccgaccggga taccgaagat acctgccgag atcggcgcga
     5041 tgccgaacca ctgcggcgca ttgacgccga agaacgggta ggtctggatc atgtagtaca
     5101 tgcagatgcc cagaccggcc aacatgccga ccaccgcccc ggtcttgttc gcgcggcgcc
     5161 agaacacgcc gcacacaagc gccgggaaga acgcggaagc cgccagcgag aacgccgcgc
     5221 cgaccaggaa caggatgtcc cccggtttca gcgaggttac gtaggcggcc agcagcgcaa
     5281 cgacgagcag cagggtcttg gcgatcgcga tacgacgagt cgtggtcgcg ttcgggtcga
     5341 tcaccttgta gtacacgtcg tgcgacagcg cgttggcgat cgtcagcagc agaccgtcag
     5401 cggtcgagag cgcggccgcc agaccgccgg ctgccaccat gcccgagatc acgtacggca
     5461 gaccggcaat ttccggcgtg gccagcacga tcaggtcacc gccgatcacg atttccgcca
     5521 gctgcacgat gccgtcgccg ttcagatccg cgatgttcat cagcgtcgga tccaccttcg
     5581 cccacgcacc cacccatgct ggcagggagg caaacgagct gcccaccagg ttatggtaga
     5641 tgtcgaactt caccagcacg gccaaggccg gggcggtgaa gtacagcagg aagatgaaga
     5701 acagcgagaa gaacaccgag cgccgcgctt ccttcacgct gggcgtggtg tagaagcgca
     5761 tcaggatgtg tggcagcgcc gcggtaccga ccatcaggca gaacaccagt gtgaggaagt
     5821 tcaggcgtgc cttgttgcgg gcttcctcgg tcggaccggc gaacggttcg gcatgggcct
     5881 tgataggacc ggcgcgggcc gcgagcccct tgtccctgtt ccacatggcc ttggcggcct
     5941 ccacatcctt cggatagttc gcgagtgctg catttgcggc gtcaacctga tctgcgggtg
     6001 cattggcgcc ggtcagctcg gcgaccttct gttccagcgc cgcacggtca gccgcgaatg
     6061 ccgcttccgg attcttcagc ttttcgttgg cggcgtcggc gcggcccttc aggatcgcgc
     6121 gcacctcggc ttccttcgcg ccgtcaggcg tgctggcgtc gttgaggacc ttttcgcggg
     6181 cactcacctt ttccagctgg acaccgtaga cgacctgcgg gatcggcacg ccggtctgct
     6241 tggccgcgag ccagacgggc gggatcatgt aggcgacgat caggatgatg tattgcgcga
     6301 cctgggtcca ggttaccgca cgcatgccac cgaggaacga gcacaccagg atgccggcca
     6361 gaccgacgaa cacaccgatg ccgaactcca ggccggtgaa gcgcgaggtg atgatgccca
     6421 caccgtagat ctgcgcaacc acgtaggtga acgacacgat gatggcagcc agcacgccga
     6481 tcaggcgcgg caggttgccg ccgtagcggg cgcccaagaa gtccggaatc gtgaactggc
     6541 cgaacttgcg caggtagggt gcgaggaaca gcgccaccag gcagtagccg ccggtccagc
     6601 ccatgatgaa ggctaggcca tcgtagccgg tggcgtagag cgtgccggcc ataccgatga
     6661 aggatgctgc cgacatccag tccgcaccgg ttgccatgcc gttgaacacc gccggcacgc
     6721 gacgacccgc cacatagtac tcggatacgt ctgcggtgcg gctcatgatg ccgatgcccg
     6781 catacaggcc gatcgtcgcg aacaggaaga tgtagccgat ccatcgggcc ggcatgccgg
     6841 cgaactcggc cagcgcgagc aggacgatga aggtgaagaa gccgccggtg tagaaggtgt
     6901 agtacttctt cagctgctgg ttaaatgccg attgactggt cgccatgttc agtcctcccc
     6961 ttcatgaacg ccatattcgt tatccagatc gttcatgtag cgcgcgtagt accagatgat
     7021 cagcacgtac acgatgagcg atccttgcgc cgccatatag aacggtagcg ggaagcccag
     7081 tagcgtgatc gaactgagtt cgcgcgcaaa gaaaccgaag aagaacgtga caaagaacca
     7141 gatcgctaac aggacccctg ttatcttcag gtttctatgc cagtactctt cgtgcttggc
     7201 agagagctgc atggttgact cctcgatata gttttgtacc ccttgcggag ctgcggaccg
     7261 actacttctg gatgccctgc gcaatcgcaa ggcggtttac tgcatcacca tggttgtgca
     7321 ccgtccttac aacaacctta caggccacac ccttcccggg tccggcggcg acattgccat
     7381 gctgcgccgc actaacgttg caggaacgat gcgcgcaaaa aaaacccgcc ttgcggcggg
     7441 ttttgtacga gcgctccggg acggagccgg gatcagacga aagctcaggc gcgcttgtcg
     7501 attgcaggga cgtcgcggcg cggcgaaccg acgaatagct ggcgcgggcg gccgatcttc
     7561 tgctccggat ccgagatcat ctcgttccac tgggcgatcc agccgacggt acgcgccatc
     7621 gcgaagatgc cggtaaacag cgagaccggg atgccgagcg cgcgctgcac gatgccggag
     7681 tagaaatcga cgttcggata cagcttgcgc tgcacgaagt agtcgtcttc cagtgcgatc
     7741 ttctccagcg ccatcgccag cttgaacagc gggtcgtcgt gcaggcccag ttcgttcagc
     7801 acttcgtggc aggtttcgcg catcagcttc gcgcgcgggt cgtagttctt gtacacgcgg
     7861 tggccgaagc ccatcagctt cacgttcgag ctcttgtcct tgaccttggc gatgaactcg
     7921 ccgatgcgct cgacaccgcc ctgctgctgg atgtcttcca gcatctgcag tgctgcttcg
     7981 ttcgcgccgc cgtgcgccgg gccccacagg caggcgatgc cagccgcaat gcaggcgaac
     8041 gggttggcgc cggacgagcc ggccaggcgc acggtcgagg tcgaggcgtt ctgctcgtgg
     8101 tcggcgtgca ggattaggat gcggtcgagc gcgcgcacca gcacgtcatt gaccttgtag
     8161 tcctcgcacg gagtcgcgaa catcatgcgc atgaagttcg cgctgtagga caggtcgttg
     8221 cgcggataga tgaacggctg gccgatcgaa tatttgtacg acatcgcgac cagcgtcggc
     8281 agcttggcga tcaggcggat cgccgacact tcgcgatgat gcgggtcgtt gatgtccagc
     8341 gagtcgtgat agaacgagga cagcgcgccg acggtaccga ccagtaccga catcgggtgg
     8401 gcgtcgcgac ggaagccgcg gaagaagaac tgcatctgct cgtgcaccat cgtgtggttg
     8461 gtcacggtgc tgacgaactt ctgcttctgg gcggcggtcg gcagctcgcc gttcagcagc
     8521 aggtagcagg tctcgaggaa gtcgcagttc accgcgagct gctcgatcgg atagccgcgg
     8581 tacagcagct cacccttgtc gccgtcgatg taggtgatcg acgaattgca ggccgcggtg
     8641 gacatgaagc ccgggtcgta tgtgaacttg ccggtcgccc cgtacagctt gcggatgtcg
     8701 atcacgtcgg gaccgatcgt gcccttgtag atcggcagat cgagcgacgg agagccgtcg
     8761 gagaacgaca gcgttgcttt ggtatcggag ttggtcatgt ctcttccttc tttgctaagg
     8821 ccaggggtga ggctggagtg aaaaaaacgg aaaaacaaaa acttcccgca ttgcagatgc
     8881 cgcctcaggc agcgcgcagg cgcgccaaca acgctcgaac atgaggctgg tcgacctcgc
     8941 cttcgggttc cttgcgcgcg agcagcaaat ccatcagttc attgtcggcc agctcgagca
     9001 agcgcgacag cgcgtcgact tcctcgtcgg tgagagcggt ctcgtgcgcg tcgagaaagc
     9061 gcgtgatgat cagatcgttt tccagcaagc cgcgccgcgc gcgccagcgc aggcgcgctc
     9121 gtttgacggg gtcggcttgg tgcgtaatcg acatgtttcc tcttgcttat ttcgtctgcc
     9181 ctgcccctgc aggacagatg aaataaggcg gaagcgaaac gcttccgccg gtgcgggtta
     9241 aaccgcgcga cgcaccatca gttccttgat cttgccgatc gcgcgcgtcg gattcagccc
     9301 cttcgggcag acatccacgc agttcatgat cgaatgacaa cggaacaggc gatacggatc
     9361 ttccagattg tccaggcgtt cgttggtcgc ttcgtcgcgg ctgtccgcga tgaagcggta
     9421 ggcctgcagc aggccggcgg gaccgacgaa cttgtccggg ttccaccaga acgacggaca
     9481 cgaggtcgag cagcacgcgc acaggatgca ctcgtagacg ccgtcgagtt cctcgcgttc
     9541 ggccggggtc tgcaggcgct ctttctccgg cggaatactg tcgttgatca ggtacggctt
     9601 gatcgaatga tactgcttga agaactgcgt catgtccacg atcaggtcgc ggatcaccgg
     9661 cagtccgggc agcgggcgca gcacgatcgg ctcggacagt tcgttcaggt tggtcgtgca
     9721 ggccagcccg ttcttgccgt tgatgttcat cgcgtcggag ccgcacacgc cttcacggca
     9781 ggagcgacgc agcgccagcg aatcgtcgac gtcggacttg atgcgctgca gggcgtcgag
     9841 cagcatcttg tcggtgtcct gcagttcgac cgtcaggtcc tgcatgtacg gcttggcatc
     9901 cttgtccgga tcgtagcggt agatcttgaa tttcagggta cgaggcatgt tctggccttt
     9961 tagaaagtac gtgctttcgg cttgaaggtt tccacggtca gcggcttggt gttgaccggc
    10021 ttgtagtcga ggcggttgcc gtcggagaac cacaaggtgt gcttcatcca gttttcatca
    10081 tcgcgcttct cgtagtcgcg atgggcatgg gcgccgcgcg attccttgcg cgccgccgcc
    10141 gagacgatgg tggccttcgc cgtttcgatc aggttgtcca gttccagcgc ttccacgcgg
    10201 gcggtgttga agaccttgga cttgtccttg aacgcgacgt gcttgcggcg ctcgtcgagt
    10261 tccatgatct tctgtacgcc ggcctgcagc agctcgtcgg tgcggaacac gccgcagtac
    10321 tgctgcatgg tggagcggat cgcgttggca acatcctgca cgcgctcgga accggtgctg
    10381 ctttccaggc gcgcgaggcg ctcgagggcg acatcgccgg cgtcggccgg cagcggcttg
    10441 tggctcttcg ccttcaggtt ctgcgcgacg atgtgattgc cggcggcgcg gccgaacacc
    10501 acgagatcca gcagcgagtt ggtgccgagg cggttcgcac cgtgtacgga gacgcaggcg
    10561 cattcgccga tcgcgtacat cccgttgacc acttcctgcg caccattctt cggcacaacg
    10621 acctggccgt ggatgttggt cgggatgccg cccatctgat agtgaatcgt cggcacgacc
    10681 gggatcggtt ccttggtcgc gtcgacgttg gcgaacttgt gtccgatttc caggatcgac
    10741 ggcaggcgct tcttgatcgt gtcggcgccg atgtggcgca gatccagcag cacgtggtcc
    10801 ttgttcgggc cgcagccgcg gccttccttg atttcctggt ccatcgagcg cgagacgaag
    10861 tcgcgcggcg ccaggtcctt cagcgtgggc gcgtaacgct ccatgaagcg ctcgccgttg
    10921 gaattgagca gg
//
LOCUS       DATLGF010000482        13132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_218085, whole genome shotgun sequence.
ACCESSION   DATLGF010000482 DATLGF010000000
VERSION     DATLGF010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 13132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13132
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218085"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="VM406_14945"
     CDS             complement(<1..1137)
                     /locus_tag="VM406_14945"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HVL77308.1"
                     /translation="MRTAKKAWIWIGGGLVALLALIVVLLTVINWNFARPWLAERIED
                     GTGRSFAIHGDLEVRWRAPEQMQTGWRRFAPWPHFRAHQIELGNPEWAETGPNMLTVP
                     QVDFTLNPLALLRRHVTISSLLFTDPELALEINEDGENNWTFRERDPESRWEVELEDL
                     FLNNGTVRLLNRARPADVTARVQTGADGSATFELEGNVAGEEVSGGGKVGALLGLRED
                     RPFPAEARVTIGDSEITAAGTVTNPRQPSALDVQLNIKGASMAQLFPFTGIALPHRPR
                     FSTEGRVVGSLARDGLHLRYEDFSGKVGSSDIGGTLEYLRREPRPLLRGKVVSQRLDL
                     DDLGALLGADDEKARKRRGEDWKQPEGRVLPARPFGTDRWDQVDV"
     gene            complement(1257..2132)
                     /locus_tag="VM406_14950"
     CDS             complement(1257..2132)
                     /locus_tag="VM406_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010809659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVL77309.1"
                     /translation="MPASTTFLLTALAMIAFAGNSLLCRLALKDTGIDAAGFTAIRLL
                     SGALTLWMLVRLRGSVPAGAGTWRSALALFAYAVFFSYAYVNLSAATGALLLFGAVQA
                     TMIGYGLWTGERFGLWRSLGLACALAGLVGLLMPGLSAPPLSGSVLMLLAGVAWGVYS
                     LFGRGVVDPLAVTAGNFNRAALLGIALAIVALPWLAPDWRGAWYAILSGALASGVGYA
                     IWYKALPALRATSAATIQLSVPVIAAAGGVVLLGEAISLRLVLASIAVLGGIALVVLV
                     KEGLPAGDAAHPRAD"
     gene            complement(2122..2568)
                     /locus_tag="VM406_14955"
     CDS             complement(2122..2568)
                     /locus_tag="VM406_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL77310.1"
                     /translation="MESLRISTAAEDQDIALIHDFLSRESAWAKGIPIELVRESIRNS
                     LNFGGFIGPAQVAYARVITDYATFAYLVDVFVLESHRGKGYADQLMRAVLDHPRLQGL
                     RRFMLATSSAHGLYAKFGFTAPVRPHTLMERFNPDAYLERRTPGAG"
     gene            complement(2617..3315)
                     /locus_tag="VM406_14960"
     CDS             complement(2617..3315)
                     /locus_tag="VM406_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HVL77311.1"
                     /translation="MTQQVKILAFDVFGTVVDWHGSVSREIDAMNLGIDGDTFALAWR
                     EGYRPAMQRVMSGETGWTLIDDLHRMILDGLLERFGVRHLSEADKCHLNRVWHRLDPW
                     PDSVHGLARLKSRFMVCSLSNGNIGLLADMAKHAGLPWDCILSAEIFRAYKPDPATYQ
                     GVARIFDVRPEQVMMVAAHQDDLAAARAAGLRTAYIERPYEFGRSRPKDVSPDPANLL
                     HATNIGHLADLLDC"
     gene            complement(3437..3910)
                     /locus_tag="VM406_14965"
     CDS             complement(3437..3910)
                     /locus_tag="VM406_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3)-I family aminoglycoside
                     N-acetyltransferase"
                     /protein_id="HVL77312.1"
                     /translation="MPTADTLEIRPLTSNELPLMHALLTMFGEAFNEVDTYGAKRPSA
                     AYLGRLLDRDDFIALVAMKNGEVIGGLAAYDLHKFEQERSEIYIYDLAVAQAHRRQGI
                     ATALILALKKIARARGAYVVFVQADGDDAQAIALYSRLGLREDVVHFDIPLSDQE"
     gene            complement(3977..4642)
                     /gene="pyrE"
                     /locus_tag="VM406_14970"
     CDS             complement(3977..4642)
                     /gene="pyrE"
                     /locus_tag="VM406_14970"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HVL77313.1"
                     /translation="MSKLRQDFIEFSVSAGVLRFGEFVTKAGRNTPYFFNAGLFNDGA
                     NLGKVADFYAQTLLDSGIEFDMLFGPAYKGITLASATAVSLARKGRNVPFAFNRKEAK
                     DHGEGGTIVGARLQGKVVIVDDVITAGTSVRESVEMIRAAGATPCAVLIALDRMERSG
                     KDGELSAHSAVQEVAKAYGMPVISIANLDDLFEYLSGAGANAELGKYKDAVAAYRRKY
                     GTA"
     gene            complement(4944..5369)
                     /locus_tag="VM406_14975"
     CDS             complement(4944..5369)
                     /locus_tag="VM406_14975"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL77314.1"
                     /translation="MKLRSAHAGDAEKLAKLFEQLGYACSTSELVERMASRVNSHNEI
                     FVAEISEDVVGVVVFNIVLPVHESGKWCVVSALVVDESVRGKGIGAALLAHVKLHAMR
                     LGCTQIELSSNESRKEAHAFYESNGFAEKRKRFVRMLGA"
     gene            complement(5405..5680)
                     /locus_tag="VM406_14980"
     CDS             complement(5405..5680)
                     /locus_tag="VM406_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77315.1"
                     /translation="MKAAQTVLGVSCIVVAAAILFAVPETYLANAGRHKDGFLFVGLA
                     EVFGELGARYFFSLPFAVLGFVVLRRAWQSKKHEANTSLHTDAKRRR"
     gene            complement(5769..6215)
                     /locus_tag="VM406_14985"
     CDS             complement(5769..6215)
                     /locus_tag="VM406_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77316.1"
                     /translation="MSPPLMSNVRAMFGAGVPSGELYFLTVLLYCLFVAVAIKHGAAL
                     FPGKDDSPWVDRKLRLLWPFTFLAILPAIIIWILFTMFGLKNVANVAFWVMWLLGLAA
                     LCYLSLFGKKDKGGASVQTVLSSLGLMCIAMLVSLAATYIFINTMF"
     gene            complement(6262..6600)
                     /locus_tag="VM406_14990"
     CDS             complement(6262..6600)
                     /locus_tag="VM406_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77317.1"
                     /translation="MFITRFGEARDWKAAEELPLPAPPEHVLSVEVDGEELQFFALPV
                     VWREASLNELIVAVADDRTGPILIANPNRGSIYAPYDGGADLFLQLSEVAMSARVMFG
                     PWLSGREDGL"
     gene            complement(6824..7663)
                     /locus_tag="VM406_14995"
     CDS             complement(6824..7663)
                     /locus_tag="VM406_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77318.1"
                     /translation="MSDEKIGLIFQTVKESRGPHMFVQYTPASENMPFASLVLVYTKE
                     VDLQEAAKKIEEEARMWFCRYPVPLVACAVGEGDDALDVSGGEGKRDLLVSSVSGEVV
                     FEWGRLGEFKMPPFSPDPQTLLRAYSSLPHSTLAQRKARTRTQALKQRAGIRLLLGIL
                     FVRRAVVPAVIALAFYLHPVLGFLGLLGALADAAVGGLRVLGYLPKSKRELAEEERRR
                     RMEHHDYHCRLNPIGFAALKAENLGASLREDAREEARRLHGEASGSQVDAIEVKPSAE
                     LQR"
     gene            complement(7807..8697)
                     /locus_tag="VM406_15000"
     CDS             complement(7807..8697)
                     /locus_tag="VM406_15000"
                     /inference="COORDINATES: protein motif:HMM:NF039499.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5677 domain-containing protein"
                     /protein_id="HVL77319.1"
                     /translation="MSLESSLAAVEVVVVDEKELSAFTRESQHFRVAFNLFRETAGYV
                     CILANTTVGPTQSWNIGQAVLGGHLVRMFKLMRFAMEESIERREEMLSVLIRLIAECV
                     INLRYLILNGSPELVRSYLAYSLQHEKELASLIRTNIKDRGGEVLPIEERMLRSIART
                     FAKSQLSEDDLPAKKIRNWGEKNLFEKAKAVGLGDAYTAIFGGPSRNVHGGWHDLLQY
                     HLEYDEPGMFRPKLEFKRPRPQPIYSLTHLIAETLEGYVDLLNHPGLSEVADRLRDLD
                     ERNYTASDAHERYLVAKHAV"
     gene            9354..10322
                     /locus_tag="VM406_15005"
     CDS             9354..10322
                     /locus_tag="VM406_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HVL77320.1"
                     /translation="MSDQPPKLLDVLKRCIGDKHYSLSMEKAYVYWCRWYVRFHDLRH
                     PRDMGAEQVRAFLSHLVNERNVAGATYTQALCALLFLYKEVLKIDLGWIEGVNRPKRP
                     PKRPTVLTQQEVTLVLEQMDGVHQLIARLLYGTGMRVSECAQLRIKDVDFGRREITVR
                     QGKGGKDRMTMLPLSLVQPLREQVATARALYEEDRALNRAGVMLPNALELKYPKAGTQ
                     LGWFWVFPSDHESTDPRSKVVRRHHVYTQTIQRAISRAVHAARITKPASTHTLRHSFA
                     THLLESGYDIRTVQELLGHTDVSTTMIYLHVLNRGGHGVISPADRL"
     gene            10453..11250
                     /locus_tag="VM406_15010"
     CDS             10453..11250
                     /locus_tag="VM406_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HVL77321.1"
                     /translation="MIKIVSANLNGIRSAAKKGFFDWMGKEQADFICVQELKAQAGDM
                     TPDFLNPHGYHGHFHYAEKKGYSGVAVYSKREPDAIQIGFGNSEFDAEGRYVQCDFGK
                     LSVISLYCPSGSSSDERQQAKFRFMEAFLPHLLKLKQCGQEVVICGDWNIAHKEIDLK
                     NWKSNQKNSGFLPEERAWLSRVFDEIGLVDAYRRLHPETTGDAYTWWSNRGQAWANNV
                     GWRIDYHITTPGIAALACKAAVYKDERFSDHAPLTVEYDGAAALLSV"
     gene            complement(11240..12139)
                     /locus_tag="VM406_15015"
     CDS             complement(11240..12139)
                     /locus_tag="VM406_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HVL77322.1"
                     /translation="MRGIAVCKRVAASAIAACALLAGPAYAEAGDGIKVDQMSSLRKL
                     VPEAELEKAAYQQYQDIKQEASKQGKLLPDHHPQVQRLRRIADQLIPHAGRWNENAAR
                     WRWEINLIQSPDINAFCMPGGKIAFFTGILDTLKLTDDEVAIVMGHEIAHALREHGRE
                     RLARSTITNAGARLLGFGLSVTLGVDPNITSTAAGVGANLAMLRFSRDDETEADLVGL
                     DLVARAGYDPRAGVALWQKMGMVNKSGAPQWLSTHPAGKTRIAEMQKHLPTVMPLYAQ
                     AKGKPLAALPPYESNVKGVGPIR"
     gene            complement(12157..>13132)
                     /locus_tag="VM406_15020"
     CDS             complement(12157..>13132)
                     /locus_tag="VM406_15020"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL77323.1"
                     /translation="LLAMASVTPTQLGLFVVLTLMVALAGATLDIAVDAYRIEIAPME
                     AQGALVATYSLGYRIALIVTGAGALVLADLAIWPHVYRAMAACMLIAVIANFVAREPE
                     VIRIKAQTWAEGMREGVVEPFLDFFRRFGGRVGLLILMFILLFKISDQALVGGLIGPF
                     YLDQGYSKTEIAAVSKVYGIWVGIAGAFVGGIAVLRLGVERALLIGIVLGAVSNLLYL
                     LLIGANGNIGMLTLVISGENFAQGILGTTAVAYLSALVNQRYTATQYALFSSLITLPG
                     KVLGFYSGRIVEAFGYAPYFWITALAIIPAVALYFWLRPRVKLGGDAA"
BASE COUNT         2672 a         4057 c         3935 g         2468 t
ORIGIN      
        1 cacatccacc tggtcccagc ggtcggtgcc gaagggacgc gccggcagca cccgcccttc
       61 cggctgcttc cagtcttcgc cgcggcgctt gcgcgccttc tcgtcgtcgg cgccgagcag
      121 cgcgcccagg tcgtcgagat ccagccgctg cgagaccacc ttgccgcgca gcagcgggcg
      181 cggttcacga cgcagatatt cgagggtgcc gccaatgtcg ctggacccga ccttgccgct
      241 gaagtcctca tagcgcaggt gcaaaccatc gcgcgccagc gagccgacga cccggccttc
      301 ggtggagaag cgcggcctgt ggggcaatgc aattccggtg aacggaaaca gctgggccat
      361 gctcgcgccc tttatattga gctggacgtc gagcgccgac ggctggcgcg gattggtcac
      421 cgtgccggcc gccgtgattt cgctgtcgcc gatcgtgacg cgggcctcgg ccggaaacgg
      481 gcggtcttcg cgcaggccga gcagcgcacc caccttgccg ccgccgctga cttcctcacc
      541 ggcgacattg ccttcgagct cgaacgtcgc gctgccgtcc gcgcccgtct ggacccgcgc
      601 ggtgacgtcg gccgggcgcg cccggttgag caggcgcacc gtaccgttgt tcaggaacag
      661 gtcttccagc tccacctccc agcgcgactc cgggtcgcgc tcgcggaagg tccagttgtt
      721 ctcgccgtct tcattgatct ccagcgccag ctcgggatcg gtgaaaagca gcgaggagat
      781 cgtgacatgc ctgcgcaaca gcgcaagcgg attgagcgtg aaatcgactt gcggcacggt
      841 gagcatgttc ggaccggtct cggcccattc cggattgccc agttcgattt gatgcgcgcg
      901 aaagtgcggc catggcgcga agcggcgcca cccggtctgc atctgttccg gcgcgcgcca
      961 gcgcacttcc agatcgccat ggatcgcgaa actgcggccg gtgccgtctt caatgcgttc
     1021 ggccagccag ggacgcgcga aattccagtt gatcacggtg aggagaacca cgatgagcgc
     1081 aagcagcgca acaaggccgc ccccgatcca gatccacgct tttttcgcgg ttcgcatgga
     1141 gatgggacca tgcggccggt ggccggttcg aacaaccggc gttaatgtgg ctcaatgcgg
     1201 tggctcaatg cggcggctca atgcggcggc tcaatgcggc ggctcaaccg gatggctcaa
     1261 tccgcgcgcg gatgcgctgc gtctccggcc gggagtcctt ccttgacgag caccaccagc
     1321 gcaatgccgc caagcaccgc gatcgacgcg agcacgaggc gcagtgagat tgcctcgcca
     1381 agcaggacca ctccgccggc ggcagcgatg accggcacgc tcagctgaat cgtggcggca
     1441 ctcgtcgcgc gcagcgccgg caaggccttg taccagatcg catagccgac gccggaggca
     1501 agtgcgccgg agaggatcgc ataccacgcg ccgcgccagt ccggcgcgag ccatggcagc
     1561 gcgacgatcg cgagcgcgat gccgagaagc gccgctcggt tgaaattgcc ggcggtcacg
     1621 gccagcggat cgacgacgcc ccggccgaac agcgaataga cgccccaggc gacgccggcc
     1681 agcagcatca gcaccgaacc cgacaacggc ggcgccgaca gcccgggcat caacaggccg
     1741 accaggccgg ccagcgcgca cgccagcccc agcgagcgcc acaggccgaa acgctcgccg
     1801 gtccagagtc catagccgat catcgtggcc tggaccgcgc caaacagcag cagcgccccg
     1861 gtggcggcgg acaggttgac gtacgcatag gaaaagaaga ccgcataagc gaacagggcc
     1921 agcgccgaac gccatgtgcc ggcgcccgcg ggaacgctcc ctcgcagccg caccagcatc
     1981 cagagcgtga gcgcaccgga cagcaggcgg atggcggtaa agccggccgc gtcgatgccg
     2041 gtgtccttca acgcgaggcg gcacagcagc gagttgccgg caaaggcgat catcgccagc
     2101 gctgtcagca ggaaggtcgt gctagccggc acctggcgtc ctccgctcca gatacgcatc
     2161 agggttgaag cgctccatca gggtgtgcgg gcgcacgggc gcggtgaatc caaacttcgc
     2221 atacagcccg tgcgcgctgc tggtggccag catgaacctg cgcagcccct gcagccgggg
     2281 atgatcaaga acagcgcgca tcagctgatc ggcatagccc ttgccgcggt gggattcaag
     2341 cacgaacacg tcgaccaggt aggcgaaggt tgcgtaatcg gtgatcaccc gcgcatacgc
     2401 cacttgcgca gggccgatga agccaccgaa gttcagcgaa ttgcggatcg attcgcgcac
     2461 cagctcgatc ggtattccct tcgcccaggc cgattcccgc gacaggaaat catggatgag
     2521 cgcgatatcc tggtcttcag ccgcagtgga aatacgcagg ctttccatgt gcatgggcct
     2581 ccggtgaagg ccgcggcgcg gccgggcgat gtgaaatcag cagtcgagca ggtcggcaag
     2641 atggccgatg ttggtcgcat gcagcaggtt tgccggatcg ggcgacacat ccttcgggcg
     2701 cgagcggcca aattcgtagg gccgctcgat gtaggccgtc ctgagccccg cggcgcgcgc
     2761 ggcggcaagg tcgtcctggt gtgctgccac catcatcacc tgctccggac gcacatcgaa
     2821 gatccgcgcg acgccttgat aggtggccgg atcgggcttg taggcgcgga agatttccgc
     2881 cgagagaatg caatcccacg gcaggcccgc atgcttggcc atgtccgcga gcaggccgat
     2941 gttgccgttg gacagcgagc acaccatgaa tcgcgacttg agccttgcga gaccatgtac
     3001 cgaatccggc cagggatcaa ggcgatgcca gacgcggttc aggtggcatt tgtccgcctc
     3061 gctgaggtgc cgcacgccga agcgttcgag caggccatcg aggatcatcc ggtgcagatc
     3121 gtcgatcagg gtccagcccg tttcgccgga catgacgcgc tgcatcgccg gtcggtagcc
     3181 ctcgcgccat gcgagtgcga acgtgtcgcc gtcgatgccg agattcatcg cgtcgatctc
     3241 gcgcgagacg ctgccgtgcc aatcgaccac ggtgccgaaa acgtcgaagg caaggatctt
     3301 cacttgctgg gtcatcgtcg ttgctccccc tctctccgga attgccggtt tcaatcccgg
     3361 accatatcat tattctcacc ctcgtccaac gaggggcgac ggcggggcag atcgacccgg
     3421 gaaaacagct acgcagtcat tcctgatcgg aaagcgggat atcgaagtgc accacgtctt
     3481 cgcgcagccc cagccgcgaa tacagtgcga tcgcctgtgc atcgtcgccg tcggcctgca
     3541 cgaagacgac ataagcgccc cgcgcccggg cgatcttctt caacgcaagg atcaaggccg
     3601 tcgcgatgcc ttgccggcga tgcgcctgcg cgaccgcaag atcgtagatg tagatctcgc
     3661 tgcgttcctg ctcgaatttg tgcaggtcat aggcggccag cccgccgatg acttcaccgt
     3721 tcttcattgc gaccagcgca atgaaatcgt cccgatcgag aaggcgcccg agataggccg
     3781 ccgaaggccg cttggcgccg taggtatcga cttcgttgaa cgcctcaccg aacattgtca
     3841 gcaatgcgtg catcagcggc agctcattcg atgtgagcgg gcgaatctcg agggtatcgg
     3901 cagttggcat ggtcttcctc ccgagcaaac gacgcgtaaa acggccgggc gcacaccacg
     3961 gcgggcggga gccgggtcag gctgtgccgt acttgcgccg gtacgccgcc acggcatcct
     4021 tgtacttgcc cagttccgca ttggcgccgg cgccggacag atattcgaac aagtcgtcga
     4081 ggttggcgat cgagatcacc ggcatgccgt aggccttcgc cacttcctgc acggcggagt
     4141 gcgccgacag ctcgccatcc ttgcccgagc gctccatgcg gtccagtgcg atcagcaccg
     4201 cgcatggggt ggcgcccgcg gcgcggatca tctcgaccga ctcgcgcacc gaggtgccgg
     4261 cggtgatcac gtcgtcgacg atcacgacct tgccctgcag cctggcgccg acgatggtgc
     4321 cgccttcgcc gtggtccttg gcttccttgc gattgaacgc gaacggcacg ttgcggccct
     4381 tgcgcgcgag cgacaccgcg gttgcggacg cgagcgtgat gcccttgtac gccggcccga
     4441 acagcatatc gaactcgatc ccggaatcga gcagggtctg ggcatagaaa tcggcgacct
     4501 tgcccaggtt cgcgccatcg ttgaacaggc cggcattgaa gaagtacggc gtgttgcggc
     4561 cggccttggt gacgaactcg ccgaagcgca gcacgccggc ggaaacggaa aattcgatga
     4621 aatcctggcg gagcttgctc acgattgcct ctgactggat ggtgacaagc cgggattgta
     4681 ccgggaggcg tgtggcgctg tacgcatggg ggtagatgtt gcgatgcgcg gcggctgtca
     4741 tgaatgggtg tgatgcggcg tggaacgccg tcgtttcgct gcaatagttg ccgtctgagt
     4801 ccaaagggca gttttgacgg ccacgttagc gtttgaggta accctacagt gccagcaggc
     4861 ggcttggtgc gcgcggctga tgataggcgg gacggtctcg cgccccaagc cgcctgctgg
     4921 tacgggtcgc gttgaccgag gggttaagcc ccaagcatcc gcacgaatcg ctttctcttc
     4981 tcagcgaacc cattgctctc atagaaggcg tgtgcctcct tcctggattc gttgctggac
     5041 agttcgattt gggtgcaacc cagcctcatt gcgtgcaact ttacatgtgc tagaagggcg
     5101 gcgccgatac ctttgcctcg aacagactca tcgaccacga gcgcagacac cacgcaccat
     5161 ttgccagatt cgtgaacagg aagaacgatg ttaaagacga ctaccccgac cacatcttcg
     5221 ctgatttccg cgacaaagat ctcgttgtgt gaattgactc tgctggccat acgttcaacc
     5281 agctccgaag tagaacaagc gtagccgagt tgctcaaaga gcttcgcgag tttttccgca
     5341 tcaccggcgt gcgcactacg cagcttcatc gcggatgggt cctcctcgga ccgaacgatt
     5401 gagttcaccg gcgccttttg gcgtcggtgt gcaacgacgt gttagcttca tgctttttcg
     5461 attgccatgc tctgcgcagc actacaaatc ccagcacggc aaacggtaag gagaagaagt
     5521 aacgcgctcc gagttcccca aaaacttctg ccagtccaac gaaaagaaaa ccatccttgt
     5581 gcctgccagc gttcgccaaa tatgtttctg gaacagcgaa cagaatggcc gctgcgacga
     5641 cgatgcaact tacacctaga accgtttgtg ctgctttcat accttaaacg ctaacgccat
     5701 attgaccggc acgcatgcgt gtcagtgtcg agtttcgagt tagagactgg ccttgtttgc
     5761 atacgggctc aaaacatggt gtttataaaa atgtacgttg ctgctaaaga aacaagcatt
     5821 gcaatgcaca tcagtccaag gctactcaac actgtttgca cggatgcgcc tcccttgtcc
     5881 ttcttgccaa agagggaaag gtagcaaagt gccgcaagcc ctaacagcca cattacccag
     5941 aatgcgacat tagccacatt cttcaagccg aacatagtaa atagtatcca aattattatt
     6001 gccggaagaa tggccaagaa tgtaaagggc cagagcagtc gaagctttct gtctacccat
     6061 ggtgaatcat ccttacctgg gaacagcgcg gccccatgct ttatcgccac ggcaacgaat
     6121 aagcaataca gcagtaccgt cagaaaatag agttctccac tcgggacgcc agcgccaaac
     6181 atagctctaa cgttcgacat gagcggcgga ctcaagcgca gcttgcggcg gtccgctcga
     6241 tggaggggtg agccaccact ctcacaaacc atcctctcgg cctgacaacc aggggccgaa
     6301 catgacgcga gcggacatag cgacctctga taattggaga aacaaatctg ctcctccgtc
     6361 gtagggagcg tagatgctgc cgcggttcgg gttcgcaatc aggattgggc ccgttcgatc
     6421 gtccgccacc gcaacgatga gttcgttgag agatgcctct cgccagacaa cgggcaaggc
     6481 gaagaactga agttcttcac catctacttc gacggacaga acatgctccg gtggagccgg
     6541 tagcggcaac tcctctgctg ctttccaatc tcttgcttcg ccaaatcggg tgatgaacaa
     6601 gaggcatttc gcatcatccc cgaggacgtg cgttgcgaca gcgttctgac gccgaagtag
     6661 ctcccaatac tcactctcgc tctccgcgta ccgcttagcc tctggaaggc tatggacacg
     6721 cagccagcga tcagcgaacg ctcggcggca aaggaaaccc acgggcggca cattgccaaa
     6781 cgccgtgagc cattgttggg cgatggggtg cggcatgtgg tggctaacgc tgaagctcag
     6841 ccgacggctt cacctcgatt gcatcgacct gtgagccgct tgcttccccg tggagtcgcc
     6901 tcgcctcttc acgggcgtcc tcgcgtaggc tcgcgccgag gttctcggcc ttcagcgccg
     6961 cgaagccaat tggatttagg cgacagtggt aatcgtgatg ctccatcctg cgacgcctct
     7021 cctcctcagc aagctcccgt ttgctcttcg gaagatagcc caacaccctc aagcctccaa
     7081 cagcagcatc agcaagggct ccgagcagac cgagaaagcc caaaacagga tgcagataga
     7141 aggcaagtgc gattacggca ggaaccaccg ctcttcgcac aaacagaatg ccgaggagca
     7201 gcctgatacc cgcccgctgc ttaagcgctt gtgttcgcgt acgggctttc cgttgggcta
     7261 gcgtactatg cggcaaggag ctataagcac gtagcagcgt ttgcgggtcc ggagagaaag
     7321 gcggcatttt gaactcgccc aagcgtcccc actcgaaaac gacctccccg cttacggagg
     7381 agacaaggag gtcacgcttc ccctcaccac ccgagacatc cagggcatcg tcgccctcac
     7441 cgaccgcaca cgcgacgagc ggcactggat agcggcaaaa ccacatccta gcctcctctt
     7501 ctatcttctt cgccgcctct tggaggtcaa cctccttcgt atagaccaac acaagcgatg
     7561 cgaaaggcat gttctcgctt gccggcgtgt actgcacaaa catgtgcggc ccgcgggact
     7621 ccttgacggt ttggaagatc aacccgattt tctcgtcact catgccaacg gcttccctag
     7681 tgcaaatggc aggttcacgg ctaacgtcgg agttgaccgg cacgcaccgg ctgggcgccg
     7741 cgaggcgtat gctcaccagc acgcttcgcg gcgcccagcc ggtgcttgtc cgcgccgaac
     7801 gacttgttag acggcatgct tcgcgaccaa gtatcgttcg tgtgcgtcgg atgccgtgta
     7861 gtttcgctca tccaagtccc tcagccgatc tgccacttcg ctgagtccag ggtggttcag
     7921 caggtcaaca taaccttcaa gcgtctcggc gatcaggtgc gtcaggctat aaataggttg
     7981 aggcctaggg cgcttgaatt cgagtttcgg tcggaacatt cctggttcgt cgtactcgag
     8041 gtggtactgc aggagatcgt gccatccgcc atgaacattg cgcgatgggc caccgaaaat
     8101 ggctgtgtat gcatccccca agccaaccgc ctttgccttc tcaaagagat tcttctcacc
     8161 ccagttccgt attttcttgg caggcagatc gtcctcggag agctgggact tcgcaaacgt
     8221 ccttgcgatg gaccgaagca tcctttcttc gatcggaagg acttcacctc cacgatcttt
     8281 gatgttggtc cggatcaggg aagccagttc tttctcgtgc tggagtgagt acgccaggta
     8341 ggagcgaacc aactccggtg agccattcaa gatcaggtac cgcaggttta tgacgcactc
     8401 cgcgatcaac cggatcagca ccgagagcat ctcctcgcgg cgttcgattg attcttccat
     8461 ggcgaatcgc atcaacttga acatccgcac caggtgccca ccaagaaccg cttgcccgat
     8521 gttccagctt tgtgtgggcc caacggtagt gtttgccaga atgcaaacgt aacctgccgt
     8581 ctcgcgaaac aggttgaagg caacgcgaaa gtgctggctc tcgcgcgtga acgccgagag
     8641 ttccttctcg tcgacgacaa cgacctcaac tgccgcgaga ctcgactcga gtgacatgcc
     8701 ttgctgccgt ctaacgtcga gctaatgggc gcgcttgcgc gtccatattc agcgacatgt
     8761 tatgagtggt cctatttttc atataccacc tccactttta ttccttgaac tacttctgct
     8821 ttggttcgcg tatcgatcct gtctttgatc ttgcacgtaa cgctaatgtg ttcatcgctc
     8881 ttccaagagt atgagagcgg ttgataacaa tatcccgcaa atacgacata tccatcgtct
     8941 ggcttccgta ctgatccact ggaagagaga acaaggtgtt gtccgtaagg ggcatggccg
     9001 ccttcggaaa gagcttccac ctcgaatacg ttcagatttc ctgaaggaga ggtgcttttc
     9061 gaaactatcg aatagtccaa attaacgagc atgtagccgc cgaaatgcca aagcataaaa
     9121 agcccgacta cgcttaacaa gaaagtttta gcgtgactta gcttcatcgt ttggcgtggg
     9181 atgactcata actgattaat taataggaca cggctgtccg tatacgtctg gcgcaaagcc
     9241 gttaagcaca gcgtcgatat tcatcaaaat atcttccata ccatagggtt gcaacgatat
     9301 ctgtctttat atacagtatt aaataatcgt caaaagcggc ttgggtcgca gccatgtcag
     9361 atcaaccacc aaaactgctc gacgtcctga aacgttgcat cggcgacaag cactacagtt
     9421 taagtatgga aaaggcctac gtctactggt gccgatggta tgtccgcttt catgaccttc
     9481 gccatcccag ggatatggga gccgagcaag tgcgggcatt cctctcgcat ttggtgaatg
     9541 agcggaatgt tgcgggggcg acgtatacgc aggccttgtg cgcactcctt ttcttataca
     9601 aggaagtact caagattgac ctcggctgga tcgaaggcgt gaaccgtcca aagcgcccgc
     9661 ccaagcgacc gaccgttctt acacaacagg aagtgacgct cgtgctggag cagatggacg
     9721 gcgttcacca gttaattgcc cgccttcttt acgggacggg aatgcgcgtt tccgagtgcg
     9781 cgcagctacg catcaaggat gtggacttcg ggcgaaggga gatcaccgtc cgccaaggta
     9841 agggcggcaa ggacagaatg accatgctgc ctctatcact ggtacagccc ttgcgcgagc
     9901 aggtggcaac agcgcgtgcg ctgtacgagg aagaccgtgc gctgaatcgc gcgggagtga
     9961 tgttgcctaa cgctcttgag ctcaagtacc cgaaagcagg cacgcagctc ggttggttct
    10021 gggtcttccc ctccgaccat gagtccaccg acccgcgttc caaggtcgta aggcggcatc
    10081 atgtgtacac gcaaaccata cagcgcgcca tctcgcgtgc agtgcatgcg gcgagaatca
    10141 cgaaacccgc ctccacgcac acgctgcgcc actcgtttgc aacccatctt cttgagtccg
    10201 gctacgacat acgcaccgtg caggagctgc ttggccacac cgacgtgtcc acgacgatga
    10261 tctatctcca tgtgctcaat cggggcgggc atggcgtgat ttcgccggcg gatcggctgt
    10321 gaacggagcg tgttgacact cggccggcga cgatgccgac ctccgtgccg gccctcccgc
    10381 ccttgccgcc gtagacgaaa gaccgccgct tttacgctat cctagccgcc tccaattccc
    10441 tacagcacgc ccatgatcaa gatcgtctcc gccaacctca acggtatccg ctccgcagcc
    10501 aagaagggct tcttcgactg gatgggcaag gagcaggccg atttcatctg cgtgcaggaa
    10561 ctgaaggcgc aggcgggcga catgacgccg gacttcctga atccgcatgg ctatcacggc
    10621 cattttcatt acgcggagaa gaagggctat tccggcgtgg ccgtctattc gaagcgcgaa
    10681 ccggatgcga tccagatcgg cttcggcaac agtgagttcg atgccgaggg tcgctacgtg
    10741 cagtgcgatt tcggcaagct gtccgtgatt tccctgtact gcccgtccgg ctcctccagc
    10801 gacgaacgcc agcaggccaa gttccgcttc atggaagcct tcctgccgca tctgctgaaa
    10861 ctgaagcaat gcggtcagga agtggtgatc tgcggcgact ggaacatcgc gcacaaggaa
    10921 atcgacctga agaactggaa gagcaaccag aagaattcgg gcttcctgcc ggaggagcgc
    10981 gcctggctgt cgcgggtgtt tgatgagatc gggctggtgg atgcatatcg ccgcctgcat
    11041 ccggaaacca ccggagacgc ctacacctgg tggagcaacc gcgggcaggc gtgggcgaac
    11101 aacgtggggt ggcgcatcga ctatcacatc accacgccgg gcattgccgc gttggcctgc
    11161 aaggcagcgg tctacaagga cgagcgcttc tccgatcacg cgccgctgac agtcgagtac
    11221 gacggcgcgg cggctcttct cagcgtatag gtccgacgcc cttcacattg ctttcatacg
    11281 gcggcagcgc ggccagcggc ttgcccttgg cctgcgcata cagcggcatc acggtcggca
    11341 ggtgcttctg catctcggcg atgcgcgtct tgccggccgg gtgggtggac aaccattgcg
    11401 gcgcgccact cttgttcacc atgcccatct tctgccatag cgcgacgcca gcacgcgggt
    11461 cgtagccggc gcgtgcaacg agatcgagcc cgaccaggtc ggcctcggtt tcatcgtcgc
    11521 gcgagaagcg cagcattgcg agattggcgc cgacaccagc ggcggtgctg gtgatgttgg
    11581 ggtcgacgcc cagcgtgacg gaaagcccga agccgagcag gcgggcgccg gcattggtga
    11641 tcgtcgatct ggccaggcgc tcgcgcccgt gctcgcgcag cgcgtgggcg atttcatggc
    11701 ccatcacgat ggccacttcg tcatcggtga gcttgagcgt atcgaggatg ccggtgaaga
    11761 aggcgatctt cccgccgggc atgcagaagg cattgatgtc cggggactgg atcaggttga
    11821 tttcccagcg ccagcgcgcg gcattttcgt tccagcggcc ggcatgcggg atgagttgat
    11881 ccgcgatgcg acgcagtcgt tgcacctgtg gatggtgatc gggcaagagc ttgccctgct
    11941 tcgaggcctc ctgcttgatg tcctggtatt gctggtaggc cgccttctcc agctcggcct
    12001 cgggcaccag cttgcgcagg ctggacatct gatcgacctt gatgccgtcg cccgcctcgg
    12061 catatgcggg gcctgccagc agcgcgcagg ccgcgattgc gcttgctgcc acgcgcttgc
    12121 acactgcaat ccctcgcatg cttgccgcca tcggcctcaa gcggcgtcgc cgccgagctt
    12181 cacgcgcggc cgcagccaga aatacaaggc aaccgcgggg atgatggcca gcgcggtgat
    12241 ccagaagtaa ggggcgtagc cgaatgcctc gacgatgcgg ccggaataga agccgagcac
    12301 cttgcccggc agcgtgatca gcgaactgaa cagcgcgtac tgggttgcgg tgtagcgctg
    12361 gttgaccagg gccgacaggt aggccaccgc cgtggtgccg agtatgccct gcgcgaagtt
    12421 ttcgccggag atcaccagcg tcagcatgcc gatattgcca ttggcgccga tcagcagcag
    12481 gtacaagagg ttgctcaccg cgccgagcac gatgccgatc agcagtgcgc gctccacgcc
    12541 caggcgcaga acggcgatgc cgccgacgaa ggcgccggcg atgccgaccc agatgccata
    12601 caccttggag acggcggcga tctcggtctt gctgtagccc tggtcgagat agaacgggcc
    12661 gatcaagccg cctaccaatg cctggtcgga gatcttgaac agcaggatga acatcaggat
    12721 cagcaggccg acgcgaccgc cgaagcgtcg gaagaaatcg aggaagggct cgaccacgcc
    12781 ttcgcgcatg ccctcggccc aggtctgggc cttgatgcgg atgacctcgg gttcgcgtgc
    12841 gacgaagttg gcaatcacgg cgatcagcat gcaggcggcc atcgcgcgat agacatgcgg
    12901 ccagatcgcc agatcggcca gtaccagcgc gccggcgccg gtgacgatca gcgcaatccg
    12961 gtacccgagc gagtacgtgg ctaccagggc gccctgcgct tccatcggcg cgatctcgat
    13021 gcggtaggcg tccactgcga tatccagcgt cgcaccggca agggccacca tcagcgtgag
    13081 cacgacgaac aggccgagct gagtaggcgt gaccgacgcc atcgccagca gg
//
LOCUS       DATLGF010000483         6027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_202609, whole genome shotgun sequence.
ACCESSION   DATLGF010000483 DATLGF010000000
VERSION     DATLGF010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202609"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VM406_15025"
     CDS             <1..521
                     /locus_tag="VM406_15025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL77324.1"
                     /translation="LAKADHGLALVRQEQVDLKHELRELFDFYGALAEEKGVRLLLEG
                     RGMLYGDRLMLRRALSNLLSNALRHTAPGMDVTVRVRDSNQTVSVCVENPGEAISEKE
                     LSRLFERFYRGEPSRSHARGEGTGLGLAIVKSIVTAHGGKISASSSAGENRFTLQFPL
                     RRRLDQQQGQPT"
     gene            749..1006
                     /locus_tag="VM406_15030"
     CDS             749..1006
                     /locus_tag="VM406_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008647613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HVL77325.1"
                     /translation="MESQQKGRRKVEPEGRWKWVLVGFLLVGGYFLWAEHKAHVIEWL
                     PYALVLLCPLMHLFMHGGHGNHDHHGHHKAALRQNDAGDEK"
     gene            1003..1659
                     /locus_tag="VM406_15035"
     CDS             1003..1659
                     /locus_tag="VM406_15035"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HVL77326.1"
                     /translation="MSEAPAYGLWSLVILNSLVFIIFAFSFTKPKTRRDWRSLGAFSA
                     FVLALFTEMYGFPLTIYLLLPWLTSHFPGIDFLSHDAGHLLEVMLGWDVNPHFGPFHL
                     LSNVFIAGGFFMLAAAWRVLYLSQRQHRLATESLYSRIRHPQYVAFVLIMFGFLLQWP
                     TLITLAMFPVLVWAYARLAKGEERDMLAEFGEEYEQYRRRVPAFIPRWSSEAGRARLS
                     "
     gene            1667..4018
                     /locus_tag="VM406_15040"
     CDS             1667..4018
                     /locus_tag="VM406_15040"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HVL77327.1"
                     /translation="MHMHAHTHDHHDHSHRPPQLGQEESDKLFTDPVCGMQVAAKPER
                     TVEHDGQAYHFCSEKCMQKFQTGPESFLQPKSAEVQPAPAGTIYTCPMHPQIRQDTPG
                     NCPICGMTLEPLMPTAEDEENPELADFRRRFWWTLPLTVIVTLVAMLAHQLLPHGLPY
                     QPWIELVLTTPVVVWAGWPFFQRWAYSIVHGSPNMWTLIGTGVGAAFLYSVVATVAPG
                     VFPPSFAVEGRVGVYYEAAAVIVSLTLLGQILELRARSQTSAAIKSLLGLAPKTARRI
                     RPDGGEEDVPITHVHVGDMLRVRPGEKVPVDGEVLEGESAIDESMLTGEPIPVTKRPG
                     DKVIGATINSGGSLIIRSEKIGSQTMLSQIVQMVAQAQRSRAPMQRMADFVAGYFVIV
                     VVAIALATLLVWGLWGPEPAWVFGLVNAVAVLIIACPCALGLATPMSVMAATGRAATQ
                     GILFRDAAAIEGMRKIDTLIVDKTGTLTEGKPAFDRTIGAPGFDDETVLRIAASIDQG
                     SEHPLAHAIVEEARRREIVLDKPESFESSSGIGVRGVVGGKRIALGNTVLMESEGINW
                     HPLAQQAEALRVEGASVMYLGADGQLVGLLAVSDPVKASTPEALATLREAGLNVIMAT
                     GDGVTTAKAVGARLGISEVYGEVKPQDKLQLVERLQAQGRKVAMAGDGINDAPALAKA
                     DVGIAMGTGTDVAINSAHLTLVKGDLRAIARARILSLQTVRNMRQNLTFAFIYNALGV
                     PIAAGVLYPVMGLLLSPLIAALAMSLSSVSVVMNALRLSRARD"
     gene            4347..4730
                     /locus_tag="VM406_15045"
     CDS             4347..4730
                     /locus_tag="VM406_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HVL77328.1"
                     /translation="MKAIANDPRLRAVFLADRRGAGGLVPVHFMRSLFAARPAVEPEA
                     FDVCPVLLAHPAEDAWTTVDASRLLFDRLGVRKDLVMLENCGHFPIEEPGVSVLRDAA
                     IRFVGRIVSDAGEATTLGKELKADV"
     gene            complement(4701..4967)
                     /locus_tag="VM406_15050"
     CDS             complement(4701..4967)
                     /locus_tag="VM406_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77329.1"
                     /translation="MKRLEILGALGAGVIGAGLGILFADRLETFAIPALLFGIAVHGW
                     AMFQKGRVQDREEQAQPGWATATEWICWVLLAALALYIGLQLLA"
     gene            complement(5071..5778)
                     /locus_tag="VM406_15055"
     CDS             complement(5071..5778)
                     /locus_tag="VM406_15055"
                     /inference="COORDINATES: protein motif:HMM:NF025559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HVL77330.1"
                     /translation="MNKRTYTRLDPLREKRRRVRILTRVVPVLLNRALKPVLGRARFA
                     FVVRMLMRAPAYRREFMHATDDPGLKEVKQTFLLVGVLYNELLARVGQDLAFRTARTF
                     LYELANAVQRQAYIPPRGTPRRWEWFHSEHEAQIVEGFIRNNENDGIRYSSSQVSLHI
                     TRCRFFEAFRDMGNAGLTEGFCRSDETVFNEFSPEMRFHRGEDVPNTIARGAARCTFI
                     YDRITPSQAVEHPAAAG"
BASE COUNT         1095 a         1814 c         1967 g         1151 t
ORIGIN      
        1 tcctggccaa ggcggatcac ggacttgcac tggtgcggca ggagcaagtc gacctgaagc
       61 atgaactgcg cgaactgttc gatttttacg gggcgttggc cgaggagaaa ggcgtgcgcc
      121 tgctgctcga aggacgaggg atgctatacg gcgaccgcct catgctgcgc cgtgcgctca
      181 gcaacttgct ctcgaacgcg ctgcgccaca ctgcgccggg catggacgtg accgtgcgcg
      241 tgcgtgactc caaccaaacg gtttcagtct gcgtggagaa cccaggagag gcgatttccg
      301 agaaagagct gtcccgcttg tttgaacgct tttaccgggg tgaaccctcg cgttcccacg
      361 cacgcggaga aggaacggga ctggggctgg cgatcgtaaa gtcgatcgtg actgcgcacg
      421 gtggaaagat atccgcgagc tcgagcgctg gagagaaccg tttcacgctc caattcccgc
      481 tccggcgccg tcttgatcaa cagcagggac aaccgacttg aacactggga gcgtgcaatt
      541 cgcggaccac cgtactcgtt tgcacgtctt tgctcgtagt gtgggaaaag gacttgaaag
      601 gcttgacttg gatcaatcaa attatgcaac tcgccaccta ctatcgactc gccttgcttc
      661 ggccgcatgg aaggctgaaa gctaccgtga accggtagta gttggaagcc gaggtccgaa
      721 acggtagttt ttttagtcgg aggcagcaat ggaatcacaa cagaaaggcc gtagaaaagt
      781 agaaccagag gggcgatgga agtgggtttt ggttgggttt ctcttagtgg gcggctattt
      841 cctctgggcg gagcacaagg cccatgtcat cgaatggctg ccctatgcgt tggtgttgct
      901 ttgtccgttg atgcacttat tcatgcatgg aggacacgga aatcatgacc accatgggca
      961 ccacaaggct gccctgcgcc aaaatgatgc gggagatgaa aaatgagtga agctcctgcc
     1021 tacgggctct ggtcgctggt aatactgaac tcactcgtgt tcattatctt tgcatttagc
     1081 ttcacaaagc cgaaaacccg tcgcgactgg cgctcactgg gggccttctc agcctttgtt
     1141 cttgcgctgt tcactgaaat gtatggattt cccctgacca tctatctcct gctaccttgg
     1201 ttgacttcgc attttccggg cattgatttt ctttcgcacg acgccggtca cctgctcgaa
     1261 gtaatgctgg gatgggacgt aaatccccac ttcggcccgt ttcacctgct aagtaatgtc
     1321 ttcattgccg gcggattctt catgttggcc gctgcttggc gtgtgctcta tctatcgcag
     1381 cgccagcacc gcctggcgac ggaaagcttg tattcccgta ttcgccaccc gcagtacgtc
     1441 gcgttcgtcc tgatcatgtt cggtttcctg cttcaatggc cgactctgat tacgctggcg
     1501 atgtttcctg tactggtctg ggcttacgcc cgcctggcaa agggggagga acgcgacatg
     1561 cttgcggagt tcggcgaaga atatgagcaa taccgtcggc gcgtaccggc tttcatccct
     1621 cggtggtcca gtgaggccgg tcgcgcgcgt ctctcatgaa aggtagatgc atatgcacgc
     1681 gcacacgcat gatcatcacg accacagcca ccgtccccca caattgggac aggaggaaag
     1741 cgacaagctt ttcaccgatc cagtttgcgg aatgcaggta gcggccaagc cggagcgcac
     1801 tgtcgagcat gacggccagg cctaccactt ctgcagcgag aagtgcatgc agaaattcca
     1861 gaccggtccg gaatcctttc tccagccgaa atctgcagag gtgcagcccg cacctgccgg
     1921 aaccatctac acctgcccga tgcatccaca gatccggcaa gacacgcccg gcaactgccc
     1981 gatttgcggg atgacgctag agcccctgat gccgacggcc gaggacgagg agaatcccga
     2041 gctggccgac ttcaggcggc gcttctggtg gacactgccg ctgaccgtca tcgtgaccct
     2101 ggtcgcgatg ctcgcacacc agttgcttcc acatgggctt ccgtaccagc cgtggatcga
     2161 gctggtgctg accacgcccg ttgtcgtatg ggccggctgg ccgttcttcc agcgctgggc
     2221 ctattcgatc gtgcatggca gcccgaatat gtggacgctg attggcacgg gggtcggggc
     2281 ggcctttctc tacagcgtgg tcgccaccgt tgccccgggc gtgttcccgc cttcttttgc
     2341 agtcgagggc cgggtcggcg tgtactacga ggctgcggcg gtgatcgtgt ccctcacgct
     2401 gctgggccag atcctcgaac tgcgcgcccg atcgcagacg tctgctgcga tcaagtccct
     2461 gctcggcctg gcgccaaaga cggcgcgccg catccggccc gatggcggcg aggaagacgt
     2521 gccgatcacg cacgtccacg tcggcgacat gctgcgtgtg cgccccggcg agaaggtgcc
     2581 ggttgacggc gaagtgctcg aaggcgaaag cgcgatcgac gaatcgatgc tcaccggaga
     2641 gccgattccg gtcaccaagc gtccgggcga caaggtgatc ggcgccacga tcaactcggg
     2701 cgggagtctc atcatccggt ccgagaagat tggatcgcag accatgctgt cgcagatcgt
     2761 gcagatggtc gcacaggccc agcgcagccg cgccccgatg cagcggatgg ccgacttcgt
     2821 cgcaggctat ttcgtgatcg tggtcgtggc cattgcgctc gcaaccctgc tggtgtgggg
     2881 tctctgggga cccgaacccg catgggtgtt cggcctggtg aacgccgtgg cggtgctgat
     2941 catcgcctgc ccgtgcgcat tggggctggc gacgccgatg tcggtgatgg cggcgaccgg
     3001 gcgcgccgca acgcaaggca tcctgttccg cgacgccgcg gccattgaag gcatgcgcaa
     3061 gatcgacacg ctgatcgtcg acaagacggg cacgctgacg gaaggcaagc cggccttcga
     3121 ccggaccatt ggtgccccgg gtttcgatga tgagacagtg ctgcggatcg cggccagcat
     3181 cgaccagggc agcgagcatc cgctcgccca cgcgatcgtc gaggaagcgc ggcggcgcga
     3241 gatcgtgctc gacaagccgg agagcttcga atcgtcgagc gggatcggcg tgcgcggcgt
     3301 cgtcggcggc aagcggattg ccctcggcaa cacggtgctg atggagtccg aaggcatcaa
     3361 ctggcacccg ctggcgcagc aggcggaagc cttgcgcgta gaaggggcga gcgtgatgta
     3421 cctgggggcc gacggccagc tcgtcggact gctcgccgtc tccgacccgg tgaaggccag
     3481 cacgcccgag gcgcttgcga cgctacgcga agcgggactg aacgtgatta tggcgaccgg
     3541 cgacggcgtg accactgcga aggccgtggg tgcccggctt ggcatttccg aggtctacgg
     3601 tgaggtcaag ccgcaggaca agctgcagct tgtcgagcgc cttcaggccc agggcaggaa
     3661 ggtggcgatg gccggcgacg gcatcaacga cgcaccggcg ctggcgaagg ccgacgtcgg
     3721 catcgcgatg ggaaccggta ccgacgtcgc gatcaacagc gcgcatctaa cgctggtgaa
     3781 gggcgacctg cgcgccattg cgcgcgcacg catcctgtcg ctccagacgg tgcgcaacat
     3841 gcgccagaac ctcaccttcg ccttcatcta caacgcgctc ggcgtaccga ttgctgcggg
     3901 tgtgctgtat ccggtcatgg ggctgctgct ctcgccgctg atcgccgcct tggcgatgag
     3961 cctgagctcg gtgtccgtgg tgatgaacgc gctgcggctg agccgggcgc gcgactgagt
     4021 tgcccgacgc tgctcgaacg catttccgca aacggactgc cggcctcctg gcaggagaac
     4081 gactggcgcg cgtacctgcg ctttcttccg ctctggttgc cgcctgggca agcaacgcgc
     4141 ctgcccgagg aagagtggtg gatggcgcgg cgcgcgcatc cacgacgacc acagccctgg
     4201 gatcatcgcg acgacgctgg ccgatctgcg ccggccgcag gcccacgccg agttcgcgcg
     4261 ccatccgttg ttggcccgct tcggcttgtc gatgctgtcc tggttcgacg ccctcacggg
     4321 cggcctgcgt ccgctggttc tcgaacatga aggcgatcgc gaacgatcct cgcttgcgcg
     4381 cagtcttcct cgccgaccgc agaggcgccg gcgggctggt tccggtgcac ttcatgcgca
     4441 gcctgttcgc cgcccgcccg gcggtcgagc ccgaagcgtt cgacgtgtgt ccggtactgc
     4501 ttgcccatcc tgcagaggac gcgtggacga cggtggacgc gagccggctg ctgttcgacc
     4561 ggctcggagt gcgaaaagac ctggtgatgc tggagaattg cggccacttc ccgatcgagg
     4621 agccgggggt gtcggtgctg cgcgatgcgg cgatcaggtt tgtcggccgg atcgtctctg
     4681 atgccgggga ggcaacgacc ctaggcaagg agctgaaggc cgatgtatag cgcaagcgcg
     4741 gcgagcagga cccagcaaat ccactccgta gccgtggccc acccgggctg cgcctgttct
     4801 tcccggtctt gcacgcggcc cttctggaac atggcccatc cgtgcacggc aatgccgaac
     4861 agcagcgccg gaatggcgaa ggtctcgagg cggtctgcga agagtatccc gaggccggcg
     4921 ccgatcacgc cggcaccgag cgcgccgaga atttccagcc gcttcatcag cgactgattt
     4981 gcagttgaag cgctcgattt catgccgggt ttcctcgtct ggtctgcatt tggatgcggg
     5041 tggcggcgag tgggcatagg ggttccggat tcagccggca gccgctgggt gctccactgc
     5101 ttgcgagggg gtgattcgat catagatgaa cgtgcaacgg gcggcgccgc gcgcaatcgt
     5161 gttcgggacg tcctctcccc gatgaaaccg catctcgggc gaaaattcgt tgaacacggt
     5221 ttcgtcggag cggcagaacc cttcggtcag gccggcgttg cccatgtcgc gaaaggcctc
     5281 gaagaagcgg caacgggtga tatgcagcga gacctggctg gacgaatagc ggatgccgtc
     5341 gttctcgttg ttgcggatga agccttcgac gatctgggct tcgtgttcgc tgtgaaacca
     5401 ttcccagcgg cgcggcgtcc cacgaggcgg tatgtaggct tgacgctgta ccgcgttggc
     5461 cagttcgtac aggaaggtcc gggctgtgcg aaacgcgaga tcctggccga cgcgcgcaag
     5521 cagttcgttg tacaggacgc caaccaggag aaaggtttgc ttgacctctt tcaggcccgg
     5581 atcatcggtc gcatgcatga actcgcgccg gtaggccgga gcgcgcatca gcatgcgaac
     5641 gacgaaagca aatcgtgcgc ggccgaggac gggtttgagg gcgcggttca gcaggaccgg
     5701 cacgacgcgg gtgagaatcc gaacccggcg ccgcttttcg cgcaatggat ccagcctggt
     5761 gtaggttcgt ttgttcatcg ctgcctcgct cttggtccat gttggccagc cggcaaacct
     5821 gcgccgggca ggtatgtcag gaagacatcc taggcaggcg cggtgcaagt tccatgacat
     5881 ggatcaaccc ggcctcgcct cggctgggcc ggcgcaatca cacccgcgca gaaggttcgc
     5941 gggccgtatg aaacacacga gaaggagaat gacatgacgt tgaggaatct gatgctgctg
     6001 gcggcgctgg ccatcccggc catgcag
//
LOCUS       DATLGF010000484         4063 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_106762, whole genome shotgun sequence.
ACCESSION   DATLGF010000484 DATLGF010000000
VERSION     DATLGF010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106762"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1340
                     /gene="pnp"
                     /locus_tag="VM406_15060"
     CDS             <1..1340
                     /gene="pnp"
                     /locus_tag="VM406_15060"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HVL77331.1"
                     /translation="EKLKTVSAQVSAALADAAAAAGESAPDGVEVGNILFDLEAKIVR
                     SQILEGEPRIDGRDTRTVRPIAVRTSVLPRTHGSALFTRGETQALAIATLGTARDEQK
                     IDALMGEYSERFMLHYNMPPFATGETGRVGSPKRREIGHGRLAKRALVAVLPAPEEFS
                     YSMRVVSEVTESNGSSSMASVCGGCLALMDAGVPLKAHVAGIAMGLIKEGNKFAVLTD
                     ILGDEDHLGDMDFKVAGTSSGVTALQMDIKIQGITKEIMQVALAQAKEARMHILTKMQ
                     EAVPQGRAELSSFAPRLITIKINPEKIRDVIGKGGAVIRALTEETGTQIDITDEGVVT
                     IASVDAAAGQEAKRRIEELTASVEIGKIYEGTVLKLLDFGAIVQVLPGRDGLLHISQI
                     ANERVNAVADYLKEGQQVRVKVLETDDRGRLKLSMKAVAAEAVAVQQGESQQQ"
     gene            complement(1404..2975)
                     /locus_tag="VM406_15065"
     CDS             complement(1404..2975)
                     /locus_tag="VM406_15065"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVL77332.1"
                     /translation="MKDEACRKSKVTNRKGTFLRERLPNGNDSIRPSRPMFTSHAAHP
                     DAPAMAHAVPAADAPGTLQQLNALLKLSPEPTLVCRRGRIVFANRAAIQLLGSVVPDS
                     IEQKPVSDYVDTPHHAPFQTSAETLACGDTQEQTWIRTDGSRFDAEVTLSCIDLDDGP
                     VLQVGVRDVSARKRAEALRTGENRVLHLIATGAGLPDILGEIATFVEHHANRGLCSIL
                     LLDSCGTKLEQRAAPSLPEGYVQRLGPTTIGPSNGSCGTAVFRREPVLVTDIASDPLW
                     ADRRGVALEFGLRACTSWPIFGHRGQVLGSFALYFREPVAPEPADLELFCAFTRLAGI
                     AIERHAEESRVRRLAHYDGLTGLPNRFLFNEFLTCALRSAQRRNRQCAVLFVDLDRFK
                     QVNDSLGHEAGDRVLRELAQRMRACLRESDKIARMGGDEFYVLIEDLQDGRHAKEVAH
                     KLIDAAARPISIDGATCTLGASVGIAVFPQDGRESDALLAKADSAMYKAKHAGRNTYC
                     FHRAAPAHTPAVLAG"
     gene            3086..>4063
                     /locus_tag="VM406_15070"
     CDS             3086..>4063
                     /locus_tag="VM406_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HVL77333.1"
                     /translation="MKAVEIAQPGGPEVLRLCERPMPRPGEGEVLIKVAAAGVNRPDV
                     LQRMGRYPVPPGASDLPGLEVAGEIVDGDLSGTSFQKGDLVCALVQGGGYAEYCVAPA
                     PQCLPVPKGLTAVEAASLPETFFTVWSNVFDRARLGPGESLLVQGGSSGIGVTAIQMA
                     AALGHPVYATAGSDEKCRACVELGARRAINYRSEDFVQALKDETGGRGVDVILDMVAG
                     GYVPREIDCLADDGRLVIIALLGGARADVDLNQILRRRLTVTGSTLRPRPSAFKGAIA
                     ENLRARIWPLLETGDIKPVIYRTFSLDQAAAAHALMESSEHIGKIVLAVE"
BASE COUNT          738 a         1219 c         1370 g          736 t
ORIGIN      
        1 ccgagaaact caagacggtg agcgcgcagg tcagcgcggc gctcgcagat gccgccgcgg
       61 cggcgggcga gtccgcgcct gacggcgtcg aagtgggtaa catcctgttc gacctggaag
      121 cgaagatcgt gcgctcgcag atcctcgagg gcgaaccgcg catcgacggc cgcgacaccc
      181 gcaccgtgcg tccgattgcg gtgcgcacca gcgtgctgcc gcgcacgcac gggtcggctc
      241 tgttcacccg gggtgaaacc caggcgctgg ccattgccac gctcggcacc gcgcgcgacg
      301 agcagaagat cgatgcgctg atgggcgagt attcggagcg cttcatgctc cactacaaca
      361 tgccgccgtt cgctaccggc gagacggggc gcgtcggctc gccgaagcgc cgcgagatcg
      421 gccacggccg cctcgccaaa cgtgcgctgg ttgccgtgct gccggcgccg gaggaattca
      481 gctactcgat gcgcgtcgtc tcggaagtga ccgagtccaa cggttcgtcc tcgatggcgt
      541 ccgtatgtgg cggctgcctg gcattgatgg atgcgggcgt gccgttgaag gcgcacgtcg
      601 caggcatcgc gatgggcctg atcaaggaag gcaacaagtt tgccgtgctg acggatatcc
      661 tgggggatga agatcacctg ggcgacatgg acttcaaggt ggccggcact tcctccggtg
      721 tgactgctct gcagatggac atcaagatcc agggcatcac caaggagatc atgcaggtgg
      781 cgctggccca ggccaaggaa gcgcgcatgc acatcctgac gaagatgcag gaagcggtcc
      841 cgcaggggcg tgcggagctg tccagcttcg cgccgcgcct gatcaccatc aagatcaatc
      901 cggagaaaat ccgtgacgtg atcggcaagg gcggtgcagt gatccgcgcg ctgaccgaag
      961 agaccggaac gcagatcgac attaccgacg agggcgtcgt gacgatcgcc tccgtcgatg
     1021 ctgccgccgg ccaggaagcc aagcgtcgca tcgaggagct gacggcgtcg gtggaaattg
     1081 gcaagattta cgaaggcacg gtgctcaagc tgctcgattt cggcgcgatc gtccaggtgc
     1141 tgcccggccg tgacggcctg ctgcacatca gccagatcgc caacgaacgg gtcaatgcgg
     1201 tagccgacta cctgaaggaa gggcagcagg tccgcgtgaa ggtgttggag accgatgacc
     1261 gcggccgcct gaaactgtcg atgaaggcgg tggctgcaga ggcagtcgct gtccagcagg
     1321 gcgagtcgca gcagcagtaa tcgccgcgtt tctctcgcaa tccgaaggcc cggctttgcc
     1381 gggctttttt ttcgccgctt gcgtcaaccg gccaggaccg ccggcgtatg agccggcgcc
     1441 gcgcgatgaa agcagtaggt attgcgaccc gcatgcttgg ccttgtacat cgcgctgtcg
     1501 gccttcgcca gcaacgcgtc gctctccctg ccgtcctgcg ggaagaccgc gattcctacg
     1561 cttgcgccca gcgtgcacgt cgccccgtca atcgaaatcg gccgtgctgc tgcgtcgatc
     1621 agcttgtgtg cgacttcctt cgcatgccgg ccatcctgca agtcctcgat cagaacatag
     1681 aattcatccc cacccatgcg cgcgatcttg tcgctttcgc gcaggcaggc gcgcatgcgc
     1741 tgcgcgagct cgcgcagcac ccggtctccc gcttcatggc cgaggctgtc attgacctgc
     1801 ttgaaacggt cgagatcgac gaacagcacc gcgcattgac ggttgcggcg ttgggcactg
     1861 cgcaaggcgc acgtcaggaa ttcattgaac aggaaacggt tcggcaggcc tgtcaggcca
     1921 tcatagtggg caagcctgcg cacccggctt tcctcggcgt ggcgttcgat cgcgatcccg
     1981 gcgagacggg tgaacgcgca gaacagctcg aggtccgcag gctccggggc taccggctcg
     2041 cgaaagtaga gtgcaaagct gcccagcacc tggccgcggt gaccgaagat cggccaggag
     2101 gtgcaggcgc gcagcccgaa ctccaacgcg acaccccgtc tgtccgccca cagcggatcg
     2161 ctggcgatgt cggtgaccag caccggttct ctgcgaaaca cggccgtgcc gcatgagccg
     2221 ttcgacgggc cgatcgtggt ggggccaagc cgctgaacat agccttcagg aaggctcggc
     2281 gctgcgcgct gctccagctt ggtgccgcac gaatccagca gcaggatcga gcagagaccg
     2341 cggttggcat ggtgctcgac gaaggtggcg atttcgccaa ggatgtcggg gaggccggct
     2401 cctgttgcga tcagatgcaa cacccgattc tcgcccgtgc gcagcgcctc tgcgcgtttg
     2461 cgggcactga catcgcgcac ccccacttgc aagaccggcc cgtcatcgag atcgatgcac
     2521 gagagggtca cctcagcgtc gaatcgcgat ccgtcggtgc gaatccaggt ctgttcctgg
     2581 gtgtcgccgc acgcaagcgt ctcggcgctc gtctggaaag gtgcatggtg gggagtatcg
     2641 acatagtccg aaacgggttt ctgttcgata gagtccggca cgacggaacc cagcaactga
     2701 atggcggcgc ggttggcgaa gacgattcgg ccgcgtcggc ataccaacgt cggctccggc
     2761 gacagcttca gaagcgcgtt cagctgttgc agtgtgcccg gcgcgtcggc ggcgggcacg
     2821 gcatgcgcca tggcaggagc atcgggatgg gcggcgtggg acgtgaacat ggggcgcgaa
     2881 gggcgaatgc tgtcgtttcc gttaggtaat ctttcccgta ggaaggttcc tttccgattt
     2941 gtaactttgc ttttcctgca agcttcatct ttcatccttt gattttgcct agcaaacacg
     3001 cggccgtatt gctgccagtc aatagagcgg aacggggagc tctcgtcagg cgtaaaatcg
     3061 tttgtttcat tagcaaggag gggtcatgaa ggctgtggag attgcccaac caggagggcc
     3121 ggaagtcctg cgcttgtgtg agcgtccgat gccgcgcccg ggtgagggtg aggtgctgat
     3181 caaggtggct gcagctgggg tgaaccggcc cgacgttttg cagcgcatgg gacgctatcc
     3241 ggtgccgccc ggggcctcgg atctgccggg actcgaagtg gccggcgaga ttgtggacgg
     3301 tgatctgagt ggcacctcat tccaaaaggg agacctcgtc tgcgcgctgg tgcagggcgg
     3361 cgggtatgcg gaatactgtg tggcgccggc gccgcagtgc ctgcccgtgc cgaaggggtt
     3421 gacagcggtg gaggcggcgt ctttgccgga gacattcttc accgtatgga gcaacgtctt
     3481 cgatcgcgcg aggttgggcc ccggcgaaag cctgctcgtg cagggtggca gttctggcat
     3541 cggcgtcacc gcgatccaga tggcggcagc gctgggtcat cctgtgtatg caacagccgg
     3601 atccgacgag aaatgccggg catgcgtaga gctgggcgcc cgccgtgcga tcaactaccg
     3661 gtcggaggat ttcgtccagg cgttaaagga tgagaccgga gggcggggcg tggatgtgat
     3721 actcgatatg gtcgcaggcg gttatgtgcc gcgcgaaatc gattgtctcg ccgacgatgg
     3781 ccgcctcgtc atcattgccc tgctcggcgg agcaagggcg gacgtcgatc tcaaccagat
     3841 cctgcggcgc cgacttaccg ttacaggatc cacgttgcga ccgcggccga gcgcgttcaa
     3901 gggcgccatt gccgaaaacc tgcgtgcacg gatctggcct ttgcttgaaa caggcgatat
     3961 caagcccgtc atctatcgca cgttttcgct cgatcaggcc gctgcggccc atgcgctgat
     4021 ggaatccagt gagcacatcg gcaagatcgt ccttgctgtg gaa
//
LOCUS       DATLGF010000485        10689 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_14989,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000485 DATLGF010000000
VERSION     DATLGF010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 10689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10689
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14989"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1475
                     /locus_tag="VM406_15075"
     CDS             <1..1475
                     /locus_tag="VM406_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008347493.1"
                     /note="catalyzes the conversion of salicylyl-CoA to
                     gentisyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HVL77334.1"
                     /translation="FERHGGAGMEQVLSAYQQVRAVEVLKIQSAARNSMEWFENVRRY
                     TRMEAQQFAYSLLTRSQRISHENLRLRDAAYVERYEDWIAERAAEQTGMPVVRGASGR
                     SIPPMLTPFRVRGTVLKNRIVVSPMAQYSAVDGVAGDYHLVHLGARAMGGAAMVFAEM
                     TCVSADARITPGCPGMYKPEHTAAWKRIVDFVHANTDAKIALQLGHAGAKGSTRCAWD
                     GIDLPLPEGNWPLVSASPQQYITGVSQLARGATREDMERIKADFVRAAQAAAEAGFDW
                     LELHCAHGYLLSSFISPLTNRRTDEYGGALENRCRYPIEVFEAIRAVWPQDKPISVRI
                     SAHDWVEGGITPDDAVRIAKLFKAAGADMIDCSSGQVSKDEKPVFGRMFQTPFADRIR
                     NEAGIPTIAVGSIFEADHANSIIAAGRADLCAVARPHLANPAWLLSETAKIGYTDTPW
                     PKQYLSGKAQLERNLERERQMAAANSGLTPQQIAAKLLEG"
     gene            1477..2268
                     /locus_tag="VM406_15080"
     CDS             1477..2268
                     /locus_tag="VM406_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVL77335.1"
                     /translation="MSAPLPLADRHALVTGGARGIGAAIADALLEQGARVTLLGRDAA
                     ALETARADMAARFGAARVATANADIARREQLERAFAEAAQRMGPIAILVNNAGQAQSA
                     PIGKTDAALWERMLAVNLTGSFHCTQLALPGMLEQGYGRIVNVASTAGLIGYSYVSAY
                     CAAKHGVVGFTRALALELATTGVTVNAVCPGFTETDIVRDAVANIVAKTGRSEQQARA
                     ELAARNPQKRLIQPEEVANAVAWLCLPASASITGQALPVAGGEVM"
     gene            2277..2807
                     /locus_tag="VM406_15085"
     CDS             2277..2807
                     /locus_tag="VM406_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVL77336.1"
                     /translation="MPRRPFAERKPEPAPLDAGAQVLDLESRLTQDDHQALKLWLRML
                     SCTVMIENEIRSRLRAEFNVTLPRFDLMAQLERHPDGLRMGELSRRMMVTGGNITGIT
                     DQLEQEKLVVRVPDPADRRAYTVKLTPAGRRAFEKMAAVHEQWITELTGALSAQEKAK
                     LLELLSKLKQGLNASE"
     gene            2909..3763
                     /locus_tag="VM406_15090"
     CDS             2909..3763
                     /locus_tag="VM406_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HVL77337.1"
                     /translation="MRYLPGEAHQLPGNSIPMTDHQARHFLFEVKDGVATLTLNRPER
                     KNPLTLDSYAELRDLFRALAYADDVKTVVITGAGGNFCSGGDVHEIIGPLTKLDMPGL
                     LTFTRMTGDVVKAMRACPQPIVAAIDGVCAGAGALLALASDIRLGTARSKTAFLFARV
                     GLAGCDMGACALLPRVIGQGRTAELMYTGRAMSGEEGERWGFFNRLCAPEDVLAQALA
                     LAASIAEGPTFAHGMTKKMLQQEWNMGVDEAIEAEAQAQAICMMTNDFHRAYHAFVAK
                     QKPVFEGD"
     gene            3885..5051
                     /locus_tag="VM406_15095"
     CDS             3885..5051
                     /locus_tag="VM406_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVL77338.1"
                     /translation="MTDTSYLSWPFFEERHRKLALELDAWAAANISQHHEPDVDAACR
                     ALVKQLGAAGWLRHAVAGTEHGAQSDTIDTRAICLIRETLARHSGLADFAFAMQGLGS
                     GAISLFGTPEQKRATLARVAAGEAIAAFALSEPEAGSDVAAMQCAAELHGDAYLLNGE
                     KTWISNGGIADFYVVFARTGEAPGARGISAFLVPADAPGFEIAERIDVIAPHPLARLR
                     FTDCRIPAANRIGESGQGFKVAMATLDVFRTSVAAAALGFARRALDEALARATARRMF
                     NQTLADFQLMQAKLAEMATGIDAAALLTYRAAWQRDQGRNVTKEAAMAKMTATETAQQ
                     VIDAAVQMHGGLGVVSEHPVERLYREIRALRIYEGATEVQQLIIARELLRAQRA"
     gene            5051..5470
                     /locus_tag="VM406_15100"
     CDS             5051..5470
                     /locus_tag="VM406_15100"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HVL77339.1"
                     /translation="MAAFRTEIPVRFADCDPAGIVFYPRYFEMFNAIVEDWCAQGWGI
                     DFHELIVERGWGLPTVRIETDFVAPSRLGERLTAQLEVARIGGASIEILLRLLGPDGS
                     DRVRSRQVLVLTDRTQGRARSLPAELRERVARFVATD"
     gene            5500..5892
                     /locus_tag="VM406_15105"
     CDS             5500..5892
                     /locus_tag="VM406_15105"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HVL77340.1"
                     /translation="MEILQPPGWARPKGYANGVAARGRMLFVSGMVGWDGEGRFHTDD
                     FAGQARQALENIVAVLKEGGAKPEHIVRMTWYVVDKQEYIAAYKAIGDAYREVIGRHF
                     PAMTAVEVGALIEDRARVEIEVTAVVPD"
     gene            5892..6743
                     /locus_tag="VM406_15110"
     CDS             5892..6743
                     /locus_tag="VM406_15110"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL77341.1"
                     /translation="MNPKRHLSKCPPDACQAQYFLRGVFTDFPQILARWQADSAAARA
                     RLPCEPDLAYAEGELCRVDVFPAREPDGSMVVFLHGGYWQGGDKSDVSFIAAAFVEHG
                     VSVAIPNYSLAPKASIDTMLAETRAAFEWLGVQAPRWSVDPARIVALGHSAGGQLAAM
                     MLTDAWAPRLGGRPLVRHAFAISGLYDLRPLRHTVLNDALGLSEESAARLSPVDHLQA
                     HGLLHPVVGELETREFHVQAALAEASWPNVAPTLSVPCLHHYTILDPLVDPASVLFGQ
                     ILAAARQ"
     gene            7165..7689
                     /locus_tag="VM406_15115"
     CDS             7165..7689
                     /locus_tag="VM406_15115"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HVL77342.1"
                     /translation="MADILPDISPARRYVVMGVSGCGKTEIGRRLAERLGAISIEGDE
                     YHSPASIEKMRAGIPLDDSDRQAWLLVLQQRLRVAREQGVAAVLSCSALKRRYRDVLR
                     GGDPELVFIHLDGDRALIEARMRTRAGHYMPPSLLESQCRDLEPLQADENGMRLGIRT
                     PPDELVEQIIGRLA"
     gene            7686..8198
                     /locus_tag="VM406_15120"
     CDS             7686..8198
                     /locus_tag="VM406_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HVL77343.1"
                     /translation="MKRLIPHLERVVEYLMALALAVMVITVFGNVVLRYGFNTGIAAA
                     EEIARLMFVWLVFLGAILALRRHAHLGVEMVQARLPRGLRRACAVVSHLLMLYGLWLF
                     LQGSWVQTQIGMSMYSTVLRYPTALLASAGLVCAASMMLIVAANLVRILVDSPKAMVP
                     GADMAAEGAR"
     gene            8195..9472
                     /locus_tag="VM406_15125"
     CDS             8195..9472
                     /locus_tag="VM406_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HVL77344.1"
                     /translation="MTLAVFLGSLGVFLTLGMPIAFALILTGVALMFHLDFFDAQLVA
                     QNLLAGADNFPLMAVPFFILAGELMNAGGISRRIINMAVAFVGHIRGGLGYVTIAAAL
                     LLASLSGSAIADTAALATLLIPMMRDNGYPVPRSAGLIASGGIIAPIIPPSMSFIIFG
                     VATNTSISKLFLAGIVPGVLMALALVAVWMWLARGMNVVPQPKRSWAERRGALADGLW
                     ALLLPVIIIGGLRGGIFTPTEAAVVAAVYALVVSLFIYREVRLRDLVQLFINASMTTS
                     IVLFLVAGAMVSSYMVTLADLPSQLGELLAPLMEDPRLLMAAIMLLLLVIGTVMDLTP
                     TILVLGPVLMPLVVKAGIDPTYFGVMFVLVGTLGLINPPVCTVLNVVCGVARISLESA
                     TRGVLPFLFVYIALVILFIVFPGIITVPMQWFF"
     gene            9487..10506
                     /locus_tag="VM406_15130"
     CDS             9487..10506
                     /locus_tag="VM406_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HVL77345.1"
                     /translation="MKTLVKLFAAAMAFSAVAVGTAAHAQDYKPRMIRFGYGLAEESN
                     QGRAVKFFADDLAKRTGNKLRVRGFASATLGSDIQMQNALVGGAQEMMVGSTATLVGI
                     VPEFGVYDLPFLFANEREADAILDGPFGQKLIAKLEEKGLVGLVYWENGFRNLTNSKR
                     PINRVEDLQGIKLRVMQNPVYIDMFNSFGANAVPLSFSELFTALETGAVDGQENPVNT
                     IQSSKFYEVQKHLALTRHVYSPWIVLASKRWWDGLSADEKKAIHETAIASRDFERRDS
                     REGAAKAVSFLKEKGMQVTEPSEAEIARMRELSKPALLKFANAGHGALINELQAELEK
                     VRKQK"
BASE COUNT         1797 a         3494 c         3611 g         1787 t
ORIGIN      
        1 gtttcgagcg ccacggcggc gccggcatgg agcaggtgtt gtccgcctac cagcaagtgc
       61 gcgcggtcga agtgctgaaa atccagagcg ccgcgcgcaa ctcgatggaa tggttcgaga
      121 acgtgcgccg ctacacgcgc atggaggcgc agcagttcgc ctattcgctg ctcacgcgca
      181 gccagcgcat ctcgcacgag aacctgcgcc tgcgcgatgc ggcctatgtg gagcgctacg
      241 aggactggat cgccgagcgg gccgccgaac agaccggcat gccggtcgtg cgcggcgcaa
      301 gcgggcgcag cattccgccg atgctcacgc cgttccgcgt gcgcggcacc gtgctgaaga
      361 accgcatcgt ggtttcgccg atggcccagt attcggcggt cgacggcgtg gccggcgact
      421 atcacctggt ccatctcggc gcgcgcgcaa tgggcggcgc cgcgatggtg ttcgccgaaa
      481 tgacctgcgt atccgcagat gcgcgcatca cgccgggctg tccaggcatg tacaagccgg
      541 agcacaccgc cgcatggaag cgcatcgtcg atttcgtcca tgcgaacacc gatgcgaaga
      601 tcgcgctcca gctcggccac gccggcgcga agggctcgac ccgctgtgca tgggacggca
      661 tcgacctgcc gcttcccgaa gggaactggc ccctggtgtc cgcctccccg cagcagtaca
      721 tcaccggcgt gtcgcaactg gcgcgaggag ccacgcgcga ggacatggag cgcatcaagg
      781 ccgacttcgt tcgcgcggca caggcggcgg cggaggccgg tttcgactgg ctggagctgc
      841 actgcgccca cggctacctg ctgtcgtcgt tcatctcgcc gttgaccaac cgccggaccg
      901 acgaatacgg cggcgcgctg gagaaccgct gccgttatcc gatcgaggtg ttcgaggcga
      961 tccgcgcggt gtggccgcag gacaagccga tcagcgtgcg catctcggcg cacgactggg
     1021 tcgagggcgg catcaccccg gacgatgcgg tccggatcgc gaaactgttc aaggcggccg
     1081 gcgcggacat gatcgactgc tcgtccgggc aggtcagcaa ggacgagaag ccggtgttcg
     1141 gccgcatgtt ccagacgccg ttcgccgacc gcatacgcaa cgaggccggc attccgacga
     1201 tcgcggtcgg ctcgatcttc gaagccgatc atgcgaacag catcatcgcc gccggccgcg
     1261 cggacctgtg cgcggtagca cgcccgcatc tggccaaccc cgcgtggctg ctgtcggaga
     1321 ccgcgaagat cggctacacc gacacgccct ggccgaagca gtacctgtcc ggcaaggcgc
     1381 agctggaacg caacctggag cgcgagcgcc agatggcggc ggccaacagc ggcctcacgc
     1441 cgcagcagat tgctgcgaag ctgctggagg gatgagatga gcgcgccact tccgcttgca
     1501 gacaggcatg cgctcgtcac cggcggcgcg cgcggcatcg gtgcggccat cgcagacgcg
     1561 ttgctggagc agggcgcgcg cgtgaccctg ctcggccgcg atgcggccgc gctcgagacg
     1621 gcgcgggcgg acatggcagc tcgcttcggc gcggcgcggg tggcaacggc gaatgcggac
     1681 atcgcgcggc gcgagcagct cgagcgcgcg ttcgccgagg ctgcccaacg aatggggccg
     1741 atcgccatcc tcgtcaacaa cgcgggacag gcgcagtccg cgccgatcgg gaaaaccgat
     1801 gcggcgctgt gggagcgcat gctcgcggtc aacctcaccg gcagctttca ctgcacgcag
     1861 ctcgcgctgc ccggcatgct cgaacagggc tacgggcgca tcgtcaatgt cgccagcacg
     1921 gccggcctga tcggctacag ctatgtcagc gcgtactgcg cggccaaaca tggagtggtc
     1981 ggcttcacgc gcgcactcgc gctggagctg gcgacgaccg gcgtgaccgt caatgcggtc
     2041 tgtccgggat tcaccgaaac ggacatcgtg cgcgatgcgg ttgcgaacat cgtggccaag
     2101 accggccgca gcgagcagca ggcgcgcgcc gagctggccg cgcgcaaccc gcagaagcgc
     2161 ctgatccagc cggaggaggt ggccaatgcg gtggcatggc tgtgcctgcc ggcatcggcc
     2221 tcgatcaccg gacaggcgct gccggttgcc ggcggcgaag tcatgtagga gccacgatgc
     2281 cgcgccgacc gttcgccgaa cgcaaaccgg aacccgcacc gctcgacgcg ggtgcgcagg
     2341 tgctggacct ggaatcgcgc ctgacgcagg acgatcatca ggcgctgaag ctgtggctgc
     2401 gcatgctctc gtgtacggtg atgatcgaaa acgagatccg cagccgcctg cgcgccgagt
     2461 tcaacgtcac gctgccgcgc ttcgacctga tggcgcagct ggagcggcat ccggatggtc
     2521 tgcgcatggg cgagctgtcc cggcgcatga tggtcaccgg cggcaacatc accgggatca
     2581 ccgatcagct ggagcaggaa aagctggtcg tgcgcgtgcc cgaccccgcc gaccgccgcg
     2641 cctacacggt gaagctgacg ccggccggac gccgcgcgtt cgagaagatg gcggccgtgc
     2701 atgagcagtg gatcacggaa ctgaccggcg cgctgtcggc gcaggaaaag gcgaagctgc
     2761 tggagctgtt gtcgaagctc aagcagggat tgaatgcgag tgaatgagcg tgtcgagcaa
     2821 gtgaggcacg gtttgcataa cagggtcagg ttccctcccc ctgaaagggg gagggaacag
     2881 tgtgccgcat agataacaag gagcccccat gcgttacctc ccaggcgaag cccaccagct
     2941 tcccggcaac tcgatcccga tgaccgacca ccaggcacgt cacttcctgt tcgaagtgaa
     3001 ggacggcgtc gcgacgctga cgctcaaccg tcccgagcgc aagaatccgc tgacgctcga
     3061 ttcctatgcc gagttgcgcg atctgttccg cgcgcttgcc tatgcggatg acgtgaagac
     3121 ggtggtgatt accggcgccg gcggcaactt ctgctccggc ggtgacgtgc atgaaatcat
     3181 cgggcctctg accaagctcg acatgcccgg cctgttgacg tttacccgca tgaccggcga
     3241 cgtcgtgaag gcgatgcgcg cctgcccgca accgatcgtc gccgcgatcg atggcgtgtg
     3301 cgcaggtgcc ggggcgttgc tggcgctggc ctcggacatc cgcctcggca ccgcgcgcag
     3361 caagaccgcc ttcctgttcg cccgggtcgg cctggccggc tgcgacatgg gcgcctgcgc
     3421 gctgctgccg cgggtgatcg gccagggccg caccgcggaa ctgatgtaca ccggccgcgc
     3481 gatgagcggc gaggaaggcg agcgctgggg cttcttcaac cggctctgcg cgccggagga
     3541 cgtgctcgcg caggcgctgg cgctggcggc gagcattgcc gaaggcccga cgtttgcgca
     3601 cggcatgacc aagaagatgc tgcagcagga atggaacatg ggcgtggacg aggcgatcga
     3661 ggccgaagca caggcgcagg cgatctgcat gatgacgaac gacttccatc gtgcgtatca
     3721 cgcgtttgtc gccaagcaga agccggtgtt cgagggggac tagcaccggt tgcaggcagt
     3781 ggactgggtt cccgcctccg cgggaacgac atggcaacgg gtgtcgcacg gcaccggatt
     3841 tccgacttcg tctcgggcgg cacaagaacg ctttgataca cacaatgacc gacaccagct
     3901 atctttcctg gccgttcttc gaagaacgcc accgcaagct cgcgctcgaa ctcgatgcct
     3961 gggccgcggc caacatcagc cagcaccatg aacccgatgt cgacgccgcc tgccgcgcgc
     4021 tcgtgaaaca gctgggcgca gccggctggc tcaggcacgc ggtggccggc accgagcacg
     4081 gcgcgcagtc cgacacgatc gacacccgcg caatctgcct gattcgtgaa accctcgcgc
     4141 gccactccgg ccttgccgac ttcgcattcg cgatgcaggg gctgggctcc ggggcgatca
     4201 gcctgttcgg cacgccggaa caaaagcgcg cgacgctggc gcgcgtggcc gccggggagg
     4261 cgatcgccgc cttcgcgctg tccgaacccg aggccggttc cgacgtggcc gcgatgcagt
     4321 gcgcggccga actgcatggc gatgcgtatc tgctcaatgg cgagaagacc tggatctcca
     4381 acggcggcat tgccgacttc tacgtggtgt ttgcgcgcac cggcgaagcg ccgggcgcac
     4441 gcggcatctc ggccttcctc gtgccggccg acgctcccgg cttcgagatc gccgagcgca
     4501 tcgacgtgat cgcgccgcat ccgcttgccc ggctgcgctt taccgattgc cgcatcccgg
     4561 ccgcgaaccg catcggcgaa tccggccagg gcttcaaggt ggcgatggcc acgctggacg
     4621 tgttccgcac gtcggtcgcg gcggcggcgc tcggtttcgc ccggcgtgcg ctggacgagg
     4681 cgctcgcacg cgcgaccgcg cgcaggatgt tcaaccagac gctggccgac ttccagctca
     4741 tgcaggcgaa actggccgag atggccaccg gcatcgatgc ggcggccctg ctgacctacc
     4801 gtgccgcctg gcagcgcgac caggggcgca acgtgacgaa ggaagcggcg atggccaaga
     4861 tgacggccac cgaaaccgcg cagcaggtga ttgacgccgc ggtgcagatg catggcggcc
     4921 tgggcgtggt cagcgagcat ccggtcgaac ggctgtaccg cgagatccgc gcgttgcgga
     4981 tctatgaagg cgcgaccgag gtgcagcaac tgatcatcgc gcgcgagctg ctgcgcgcgc
     5041 agagggcctg atggcggcct tccgtaccga gattccggtg cgctttgccg actgcgatcc
     5101 ggccggcatc gtgttctatc cgcgctactt cgaaatgttc aacgcgatcg tcgaggactg
     5161 gtgcgcgcag ggatggggca tcgactttca cgagctgatc gtggaacgcg gctggggatt
     5221 gccgacggtg cgcatcgaga ccgatttcgt cgccccgagc cggctcggcg agcgcctgac
     5281 cgcgcagctg gaagtggcgc gcatcggcgg cgcctcgatc gagatcctgc tgcggctgct
     5341 agggccggac ggctcggacc gcgtgcgctc gcgccaggtg ctggtgctga ccgaccggac
     5401 gcagggccgt gcgcggagcc tgccggcgga actgcgcgaa cgcgtcgcgc gcttcgttgc
     5461 gacagactga atttctggca aaccaagaga ggttcaaaca tggaaatcct tcagccaccg
     5521 ggatgggcac ggcccaaggg gtatgcgaac ggcgtggccg cgcgcgggcg gatgctgttc
     5581 gtcagcggca tggtcggatg ggacggcgaa ggccgctttc ataccgacga tttcgccggg
     5641 caggcgcgcc aggcgctgga gaacatcgtc gccgtgctca aggaaggcgg cgcgaagccc
     5701 gagcatatcg ttcgcatgac ctggtacgtg gtcgacaagc aggagtacat cgccgcgtac
     5761 aaggcgatcg gcgacgcgta tcgcgaagtg atcggccggc acttcccggc aatgacggcg
     5821 gtcgaagtcg gcgcactgat cgaagatcgc gcgcgcgtgg aaatcgaagt gacggcggtg
     5881 gtgccggact gatgaacccg aagcgccatc tctcgaagtg cccgccggac gcctgccagg
     5941 cgcagtattt cctgcgcggg gtgtttaccg attttccgca gatcctcgca cgctggcagg
     6001 ccgattcggc ggccgcgcgc gcgcggctgc cctgcgaacc cgaccttgcc tatgccgaag
     6061 gcgagctgtg ccgcgtcgac gtctttccgg cgcgtgaacc ggacggctca atggtcgtct
     6121 tcctccatgg cggctactgg cagggcggcg acaagtccga cgtcagcttc attgcggccg
     6181 ccttcgtcga gcacggggta tcggtcgcga ttccgaatta ttcgctcgca cccaaggcct
     6241 cgatcgacac gatgctggcc gaaacgcgtg cagcgttcga atggctgggt gtccaggcgc
     6301 cgcgctggtc cgttgatccg gcgcgcatcg tcgcgctcgg ccattcggcc ggagggcagc
     6361 ttgccgcgat gatgctgacc gatgcctggg ccccgcggct cggcggacgt ccgctggtgc
     6421 ggcacgcgtt cgcgataagc ggcctgtatg acctgcggcc gctgcgccac accgtgctca
     6481 acgatgcgct cggcctgagc gaggagagcg cggcgcgcct cagcccggtg gaccacctgc
     6541 aggcgcacgg cctgctgcat ccggtcgtgg gcgaactgga gacgcgcgag ttccatgtcc
     6601 aggccgcgct tgcggaggcg agctggccga atgtggcgcc cacgctctcc gtgccgtgcc
     6661 tgcaccacta tacgatcctc gatccgctcg tcgatccggc cagcgtgctg ttcgggcaga
     6721 tcttggcagc cgcccggcag taggactgtt agcgttaaac tacggcgcgg aagctggaaa
     6781 gggtgcctct tcgatgggtt ggcgcgcgtc agcccgctct ttctgccgcg ctgacttagc
     6841 catgaactac cggcagctgg cggtccgcag ccagttgctg ttcacctgtg tgactgcctc
     6901 cgccatatcc tccccctagg cgggcccccg ctcagtcggg ggagccgcca cgatcgcctg
     6961 cgcaacgtcc gccggcgccg gatatggccc ccacctgcac gggggcgcag gtgcggggtc
     7021 gtcggcgcag cgaatggcgt tcatggacgc cggccctttc gacgccgacg acaaaaaatc
     7081 cctttacaaa ccgccaagta acgctaaaat gcgttccaat gttagcgata acaacaaaaa
     7141 caaacacaag agttcgagag acgcatggcg gatatcctcc ccgacatttc ccctgcccgg
     7201 cgctacgtcg tgatgggcgt atccggctgc ggcaagaccg agatcggccg acgcctggcc
     7261 gagcggctgg gcgcgatcag catcgagggc gatgagtatc actcgccggc cagcatcgaa
     7321 aagatgcgtg caggcatccc gctggacgac agtgaccgcc aggcctggct gctggtgctg
     7381 cagcagcggc tgcgcgtggc gcgcgagcag ggtgtcgcgg cggtgttgtc ctgcagcgcg
     7441 ttgaagcgcc gctaccgcga cgtgctgcgc ggcggcgatc ccgaactcgt cttcattcac
     7501 ctcgacggcg accgggcctt gatcgaggcg cgcatgcgca cccgcgccgg gcactacatg
     7561 ccgccatcgc tgctggagag ccagtgccgc gatctcgaac cgctgcaggc ggacgagaac
     7621 ggcatgcgcc tgggcatccg cacgccgccg gacgagctgg tcgagcagat catcgggagg
     7681 ctcgcatgaa gcgcctgatt ccccaccttg agcgcgtcgt cgagtacctg atggcgctgg
     7741 cgcttgccgt gatggtgatc accgtgttcg gcaacgtggt gctgcgctac ggtttcaata
     7801 ccggcatcgc cgccgccgag gaaatcgcgc ggctcatgtt cgtctggctg gtcttcctcg
     7861 gggcgatcct ggcgctgcgc cgccatgcac atctcggtgt cgaaatggtg caggcacgcc
     7921 tgccgcgcgg gctgcgccgc gcctgcgcgg tggtgagcca tctgctgatg ctgtatggcc
     7981 tgtggctgtt cctgcaggga agctgggtgc agacccagat cggcatgagc atgtattcga
     8041 cggtgctgcg ttacccgacc gcgttgctgg cgtcggctgg gttggtgtgc gcggcctcga
     8101 tgatgctcat cgtcgcagcc aacctcgtgc gcatcctggt cgacagcccg aaagccatgg
     8161 tgccgggagc ggatatggct gcggagggcg cacgatgaca ctggcggtct tcctcggttc
     8221 gctgggcgtg ttcctgacgc tcggcatgcc gatcgcgttc gcactgatcc tgaccggcgt
     8281 ggcgctgatg ttccacctcg acttcttcga cgcccagctg gtcgcgcaga acctgcttgc
     8341 cggcgccgac aatttcccgc tgatggccgt gcccttcttc atcctggccg gggagctgat
     8401 gaatgcgggc ggcatctcgc gtcgcatcat caacatggcg gtcgccttcg tcggccacat
     8461 ccgcggcggt ctcggctacg tgaccatcgc cgcggccctg ctgctggcga gcctgtccgg
     8521 ctcggcgatc gccgataccg cggcgctggc cacgctgctg attccgatga tgcgcgacaa
     8581 cggctacccg gtgccgcgct cggccggact catcgcatcc ggcggcatca tcgccccgat
     8641 catcccgcct tcaatgtcct tcatcatctt cggcgtcgcg accaacacgt cgatcagcaa
     8701 gctgttcctg gccgggatcg tgccgggcgt gctgatggcg ctcgcactgg tcgcggtctg
     8761 gatgtggctt gcacgcggga tgaatgtcgt cccacagccg aagcgctcct gggccgagcg
     8821 ccgtggcgcg ctggccgacg ggctgtgggc gctgctgctg ccggtcatca tcatcggcgg
     8881 tctgcgcggc gggatcttca cgccgaccga ggccgccgtg gtggcggccg tctatgcact
     8941 ggtcgtcagt ctgttcatct accgcgaggt caggctgcgc gatctcgtac aactgttcat
     9001 caacgcgtcg atgaccacca gcatcgtgct gtttctcgtc gctggggcaa tggtgtcgtc
     9061 ctacatggtt acgctggccg atctgccgtc ccagctcggc gaactgctcg caccgctgat
     9121 ggaagacccg cgcctgctga tggccgcgat catgctgctg ctcctcgtca tcggcacggt
     9181 gatggacctc acgccgacca tcctcgtgct cggcccggtg ctgatgccgc tcgtcgtgaa
     9241 ggccggcatc gatccgacct acttcggcgt gatgttcgtg ctggtgggca cgctgggact
     9301 gatcaatccg cccgtgtgca cggtgctcaa cgtggtctgc ggcgtcgcgc gcatttcgct
     9361 ggagagcgca acgcgcggcg tgctgccgtt cctgttcgtc tatatcgcac tggtcattct
     9421 gttcatcgta ttccccggga tcatcacggt cccgatgcag tggttctttt aattacagga
     9481 ggagtcatga aaacgctggt caaactattc gccgccgcaa tggcattttc cgcggtcgcc
     9541 gtcggcaccg ccgcccacgc ccaggactac aagccgcgca tgatccgctt cggctacggg
     9601 ctggccgaag agagcaacca gggccgggcc gtcaagttct tcgccgacga cctcgccaag
     9661 cgcaccggca acaagttgcg cgtgcgcggc ttcgcatccg ccacgctggg cagcgatata
     9721 cagatgcaga acgcgctggt gggcggtgcc caggaaatga tggtcggctc caccgcaacg
     9781 ctggtcggca tcgttcccga gttcggcgtg tacgacctgc ctttcctgtt cgccaatgag
     9841 cgcgaagccg atgctatcct cgacggcccg ttcggccaga agctgatcgc gaagctggaa
     9901 gagaaagggc tggtcggcct ggtctactgg gagaatggtt tccgcaacct gaccaactcg
     9961 aagcgcccga tcaaccgcgt cgaagacctc caggggatca agctgcgcgt gatgcagaac
    10021 ccggtctaca tcgacatgtt caacagcttc ggcgcgaacg cggtgccgct gtcgttctcc
    10081 gagctgttca cggcgctgga aaccggcgcg gtcgacggcc aggaaaaccc ggtcaacacg
    10141 atccagtcga gcaagttcta tgaagtgcag aagcatctgg cgctgacccg ccacgtgtac
    10201 agcccgtgga tcgtgctcgc gtccaagcgc tggtgggacg gcctctcggc cgatgagaag
    10261 aaggcgatcc atgagacggc gattgcctcg cgcgacttcg agcgccgcga cagccgggaa
    10321 ggcgcggcca aggcggtgtc cttcctgaag gaaaagggca tgcaggtcac cgagccgagc
    10381 gaggccgaaa tcgcgcgcat gcgcgagctt agcaagccgg cgctgctgaa gtttgccaat
    10441 gccggccatg gtgcgctgat caacgaactg caggcggaac tggagaaggt gcgcaagcag
    10501 aagtaggaac ggcttcttct tgaagtaaat cggaaagaag tttgaaccac ggcgggcacg
    10561 gcggacatgg cacgacgctt cccctttcat tccgagccac ctttctgtcg ttccgtgcca
    10621 ccttcctgtc attccgagcg aagcgaggaa tctcatgcac gcaattcgat gagatccttc
    10681 gctgcgctc
//
LOCUS       DATLGF010000486         3113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_15028,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000486 DATLGF010000000
VERSION     DATLGF010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15028"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..>3113)
                     /locus_tag="VM406_15135"
     CDS             complement(35..>3113)
                     /locus_tag="VM406_15135"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HVL77346.1"
                     /translation="VLDVDLVAEDDGSGSLRLRNAAPGTIEDGRMPLQAMDVDFRQRE
                     GGLDLERIVARLGSAAPAARAAGELRGSGRIGDGAVNLSLKTERFDLARIDPGLRSTQ
                     LAGSIDAGHDNGAQEVVVALTEPFGRQRIVLDAHARIADAVVELRHMELRAGAGRLRA
                     SGRVALEGTQAFHAEGEVDRFRLHDFGRFEEVPAMDLNGRFKLDGERSPQLRADLAFT
                     IADSRLAGQPLAGEGRATLHGEQLEVPRLLLSSGSNRLEAQGRLADGNGELKFSIQAP
                     QLGQLGPAFGGALFADGVARGSLEQPSISAQWRAERTRLPGAVRIGASQGQADLQLDR
                     SAPLMLRAGEAAFEASELRREEQRIERLDVRLRLSPQPDAPLAFDMEARGLHVGGAQF
                     AQLDINIDGTTARHTVRANAQEDGQTWQLRAAGGLDAVGADASWQGNLEAFEARGPIN
                     ARTSGSAPLLLSREAVQVENLELAMDGGRLQLERFRRDDSGIETRGRIEQLQLAEFLR
                     FAGPERPLDTNLQLSGSWDLRYRDQLDGNLALQRDSGDITVLGTTPVQLGLTTLRAAM
                     RADAGRVALDAQAAGTRMGSIALDARTRLGADGNPLALPPDAPLAGSARIEVPSLAWL
                     APIVMPELSADGRINGQVALGGTIGEPAISGRIDGDALRVAHPEYGLDLREGTLRSEF
                     RGDELVLHGLTFNGAQGRLHLAGPIQLGEDMRANLRLQAERFAVLNRPDRRLVLSGQS
                     RINWQQQRAMVIGEFTVNSGFFDIGQADRPTLGDDVVIVGREERAQRGMAAAIDITVR
                     LGDGVTVRGRGLDALVAGQARILSDTGEPPQAQGTLRVVKGTFSAYGRELGIERGELR
                     FTGPLDNPALDILAMRRGQEVEAGVAVGGNVLTPRITLVSEPVVPDAEKLAWLVLGRS
                     LASAGAGDLGSLQDAAASLLAQGAIAGVQSRIASAFGLDTFAIGTDENNLQQRIVTIG
                     RQISSRLYLGYQHGLESAASVVQLRYLLTPTLSLEAEAGARSALSLFYNIAFD"
BASE COUNT          481 a         1088 c         1051 g          493 t
ORIGIN      
        1 ccgatgaacg gggacagaag ccgtgccaag gggttcagtc gaatgcgatg ttgtagaaca
       61 atgacagcgc gctgcgcgcg ccggcttcgg cttcgagcga cagggtgggc gtgagcaggt
      121 agcgcaactg caccacgctg gccgcacttt cgagtccgtg ctgatagccg aggtagagcc
      181 gcgaggaaat ctgccggccg atcgtgacga tgcgttgctg cagattgttt tcgtcggtgc
      241 cgatcgcaaa ggtatccagg ccaaacgcgc tggcaatgcg cgactggacg ccggcaatgg
      301 caccctgcgc aagcagggag gcggcggcgt cctgcagcga gccgagatcg ccggcgccgg
      361 cactcgcgag gctgcgcccg agcaccagcc aggccagttt ttccgcatca gggacgacag
      421 gttccgacac cagcgtgatg cgtggagtca gaacgttgcc gccgaccgcc acaccggcct
      481 ccacttcctg tccgcggcgc atcgcaagga tgtcgagcgc cggattgtcg agcgggccgg
      541 taaagcgcag ttccccccgt tcgatcccga gttcgcgccc atatgcactg aatgtgccct
      601 tgaccacccg cagcgtcccc tgggcctggg gcggctcgcc agtgtcgctc aggatgcgcg
      661 cctggcccgc gaccagcgca tccagtccgc gcccgcgtac ggtcacgccg tcgcccaggc
      721 gtaccgtgat gtcgatggcg gcggccatgc cgcgttgcgc acgctcctcg cgcccgacga
      781 tgacgacgtc gtcgcccagc gtcgggcggt cggcctggcc gatgtcgaag aagccggaat
      841 tgacggtgaa ctcgccgatg accattgcgc gctgctgttg ccagttgatc cggctctggc
      901 cggaaagcac gaggcgtcgg tcgggccggt tcaggaccgc gaagcgctcg gcctgcaggc
      961 gcaggttcgc gcgcatgtcc tcgcccagct ggatcgggcc ggccaggtgc agccggccct
     1021 gggcgccgtt gaaggtcaaa ccgtgcagca ccagttcgtc gccgcggaat tcgctgcgca
     1081 gcgtaccttc gcgcaggtcc aggccatatt ccggatgggc cacgcgcaac gcatcgccgt
     1141 cgatgcggcc gctgatcgca ggctcgccta tcgtcccgcc cagcgcgacc tgcccattga
     1201 tacgaccgtc ggccgagagc tcgggcatca cgatcggcgc cagccaggcc agcgagggca
     1261 cttcgatgcg ggcggatccc gccagcggcg cgtcgggcgg aagcgcaagc gggttgccgt
     1321 ccgcgccaag cctggtgcgc gcatcgagcg cgatgctgcc catgcgcgtg cccgcagcct
     1381 gcgcatccag cgccacacgg ccggcgtcag cccgcatcgc tgcgcgcagc gtcgtaaggc
     1441 caagctggac cggtgtggtg ccgagcacgg tgatgtcgcc gctgtcgcgc tgcagcgcca
     1501 ggttcccgtc gagctgatcg cgatagcgca agtcccagct gccgctcaac tgcaggttgg
     1561 tgtccagcgg ccgctcgggg ccggcgaagc gcaggaactc ggccagctgc agttgctcga
     1621 tgcgcccgcg cgtttctatt ccgctgtcgt cgcggcgaaa acgctccaat tgcagccggc
     1681 ctccatccat tgccagctcc aggttctcga cctgtacagc ctcgcgtgac agcagcagcg
     1741 gggcgcttcc agacgtgcgg gcgttaatgg gtccgcgcgc ctcgaacgcc tcgagattac
     1801 cctgccagct tgcatcggct cccacggcat ccaggccgcc ggcagcccgc agctgccatg
     1861 tctgtccatc ttcctgcgcg ttggcacgca cggtatggcg ggcggtcgtg ccatcgatgt
     1921 tgatgtcgag ttgcgcgaac tgcgcgccac cgacatggag cccgcgcgct tccatgtcga
     1981 aggcgagcgg tgcatcgggc tgcggggaca gccgcagtcg cacgtccaga cgctcgatcc
     2041 gctgttcctc gcgacgcagt tccgatgctt cgaatgccgc ttcgccggca cgcagcatca
     2101 gcggcgcgga tcggtcaagt tgcagatcgg cctgcccctg ggaagcaccg atgcgcacgg
     2161 ctcccggaag ccgggtacgc tcggcacgcc actgcgcact gatcgacggc tgttcgaggc
     2221 tgccgcgcgc cacgccatct gcgaacagtg cgccgccgaa cgccggaccg agctgtccga
     2281 gttgcggcgc ctggatcgaa aacttcagct cgccgttgcc gtcggcaagc cgcccctgag
     2341 cctccagccg gtttgaaccg gagctcagca gcaggcgcgg aacttccagc tgttcgccgt
     2401 gcagcgtggc gcggccttcg ccggcaagcg gctgtccggc tagccggctg tcggcgattg
     2461 tgaaagccag gtcggcgcga agctgcgggc tgcgctcgcc gtcgagcttg aagcggccgt
     2521 tcaggtccat cgccggaacc tcctcgaagc ggccgaagtc gtgcaggcgg aagcggtcga
     2581 cttcgccctc ggcatggaag gcctgagtgc cttccagcgc aacgcggccc gacgcgcgta
     2641 gccggcccgc gcctgcgcgc agctccatgt ggcgcagctc gaccaccgca tctgcaatgc
     2701 gcgcgtgcgc gtcgaggacg atgcgctggc gtccgaacgg ctccgtgagc gccaccacca
     2761 cctcctgcgc cccgttgtca tgaccggcat cgatggagcc ggcgagctgg gtgctgcgca
     2821 gccccgggtc gatgcgcgcc aggtcgaaac gttcggtctt gagcgacagg ttcaccgcgc
     2881 cgtcgccaat acgtccgctg ccgcgcagtt cgccggctgc gcgcgcggcg ggggcggcgc
     2941 tgccgagtcg cgcaacgatc cgttcgagat ccagtccgcc ttcacgctgg cgaaaatcaa
     3001 catccattgc ctgcagcggc atccggccat cctcgatggt gcccggggcg gcattgcgca
     3061 ggcgcaagct gccgctgccg tcgtcttccg caacgagatc gacatcaagc acg
//
LOCUS       DATLGF010000487         3087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_185614, whole genome shotgun sequence.
ACCESSION   DATLGF010000487 DATLGF010000000
VERSION     DATLGF010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185614"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VM406_15140"
     CDS             <1..263
                     /locus_tag="VM406_15140"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HVL77347.1"
                     /translation="PSFTFEGVRGTRPVLIAVFAVFGLQLLFTYAPLMRTLFQTEPLD
                     FVTGLQIVGVGVLLLVILEVEKFLRRGLFPGPATARGEERTV"
     gene            407..952
                     /locus_tag="VM406_15145"
     CDS             407..952
                     /locus_tag="VM406_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012760927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL77348.1"
                     /translation="MTSTFRIERLIRADEQVLAGLTDVLLDCVEGGASVSFMLPLPRS
                     RAEAFWRGVLAQAESGERILLIASDDAGIVGTVQVVPAQPENQPHRADISKLLVHRRA
                     RRRGIAEALMREAETAALACDKTLLVLDTATPGDAERLYARLGWERCGTIPDYALWPD
                     GRLAATALFYKRLGQRFMHLA"
     gene            complement(936..1340)
                     /locus_tag="VM406_15150"
     CDS             complement(936..1340)
                     /locus_tag="VM406_15150"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HVL77349.1"
                     /translation="MHTPESLQHLLAPLFPGLLGMRIDEVGAERVKASLPVREGLCTV
                     GGILHGGALMALADTLGAIGAFVNLPEGKRTTTIESSTKFVGAARAGTTVSAECVVLH
                     RGRTTTVWQTSVYAEDGRLCAIVSQTQLTLDA"
     gene            complement(1351..2223)
                     /locus_tag="VM406_15155"
     CDS             complement(1351..2223)
                     /locus_tag="VM406_15155"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVL77350.1"
                     /translation="MNEPRLSAPSASRYFSDFASALRYWRSKRGFSQLRLATESGISQ
                     RHISFLESGRAQPSRELIIKLGIVLDIPLRQRNVMLHAAGFAAAYQERNLSDPELKSV
                     KQALDFMLAQQQPYPALVVDRLWNLVMANAAAAGMMKWLLDMPADMPIPSEGVNVLKL
                     MLDPNGARRHVCNWEEVSSDLVHWIQREAMSDGPGSEAAQLLAEMVAFPGVKAATGTV
                     NLEMRALPFLPVKIRKDGVELNLFTTITTLGTPHDVTVHELRVECYFPADDASAAWFR
                     DGRFAGSADLLKTA"
     gene            2373..2693
                     /locus_tag="VM406_15160"
     CDS             2373..2693
                     /locus_tag="VM406_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HVL77351.1"
                     /translation="MSAYAVGHLEHVNMGQPIVDYLRNIDATLAEYGGRFIIHGGRTD
                     VLEGSFRGDLIVIEFPDLARARAWYASPAYREILPLRTENASGAVFLIEGVGPDHKAT
                     DVLG"
     gene            complement(2694..>3087)
                     /locus_tag="VM406_15165"
     CDS             complement(2694..>3087)
                     /locus_tag="VM406_15165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL77352.1"
                     /translation="IAGFAGAALWLPPGIEPDKQQILDVLRSSVGPDRLPTLMAVFDE
                     MGSSRPEEPHWYLPVIGVDPAYQGRGHGHALLHHGLERVDDEGKIAYLENTNPDYTRL
                     YESHGFRVVGTIEVAEAPPLIQMLRPAQ"
BASE COUNT          505 a         1010 c         1026 g          546 t
ORIGIN      
        1 cgccttcgtt taccttcgaa ggtgtgcgcg gcaccaggcc ggtgctgatc gccgtgttcg
       61 cggtcttcgg cctgcagctg ctgttcacct atgcgccgct gatgcgaacc ttgttccaga
      121 ccgagccgct ggactttgtc acggggctgc agatcgtcgg cgtcggcgtg ttgctgctgg
      181 tgatactgga agtggagaag ttcctgcggc gcggcctgtt tcccggccct gcaacagcac
      241 gcggagagga acgcacggtg tgatcctcat gtcggccgac gattcgggct tggggtgcgg
      301 gtcgcttgtg cgatgcttct gtcgcccccg cgaaggcgcg ggccgagctc ctacacctgc
      361 acggctgcga taattctcac cacgcgaaca agaaaaagga gcccgcatga caagcacctt
      421 ccggatcgaa cgcctcattc gtgccgatga gcaggtgctc gcaggattga cagacgtgtt
      481 gctcgactgc gtggaaggcg gggcatcggt gagcttcatg ctcccgctgc cgcgttcgcg
      541 cgccgaggcg ttctggcgcg gcgtgctggc gcaggcggaa tccggcgaac gcatcctgct
      601 gattgcctcg gacgacgccg gcatcgtcgg caccgtgcag gtggtgccgg cgcagcccga
      661 gaatcagccg caccgcgccg atatctccaa gctgctggtg caccggcgcg cgcgcaggcg
      721 gggcatcgcc gaagcgctga tgcgcgaggc cgagacggcg gcgctcgcct gcgacaagac
      781 gctgctcgtg ctcgataccg ccacgcccgg cgacgcggag cgcctgtatg cgcggctggg
      841 ctgggagcgc tgcggaacga ttccggacta tgcgctatgg ccggacggaa ggctcgcggc
      901 gaccgccttg ttctacaagc ggctgggaca gcgattcatg catctagcgt gagttgggtc
      961 tgactgacga tcgcgcacag tcgtccgtcc tccgcgtaca cgctggtctg ccagaccgtc
     1021 gtggtacggc cgcgatgcag caccacgcat tcggcgctga cggtggtgcc ggcacgggcg
     1081 gccccgacaa acttcgtgct cgactctatc gtggtcgtgc gcttgccttc ggggagattc
     1141 acgaacgcgc cgatcgcccc cagcgtgtcg gccagcgcca tcagtgcgcc gccatgcagt
     1201 atgccgccga cggtgcacag gccctcgcgc accggcagcg aggctttcac gcgctcggcg
     1261 ccgacctcgt caatgcgcat gccaagcaag ccgggaaaca gcggcgcgag caggtgctgc
     1321 agcgattcgg gcgtgtgcat gttcgtcctt tcaggcggtc ttcagcaagt cggcggatcc
     1381 ggcgaagcgg ccatcgcgga accacgcggc gctcgcatcg tcggccggga agtagcactc
     1441 gacgcgcagc tcatgcaccg tcacatcgtg cggcgtgccc agtgtcgtga tcgtggtgaa
     1501 caggttcagc tcgacgccgt ccttgcggat tttcacggga agaaagggca gcgcgcgcat
     1561 ttcgagattg accgtaccgg tcgcggcctt gacaccgggg aacgcaacca tctcggcgag
     1621 cagctgcgcg gcctcgctgc cgggaccgtc gctcatcgcc tcgcgctgta tccagtgcac
     1681 gaggtcggat gacacttcct cccagttgca gacgtgccgg cgcgcgccgt tcggatcaag
     1741 catcagtttc agcacgttga caccctcgga cgggataggc atgtccgccg gcatgtcgag
     1801 cagccacttc atcatccccg ccgcggccgc attggccatc acgaggttcc acagccggtc
     1861 gaccacgagc gccggatagg gctgctgctg cgcgagcatg aaatccagcg cctgcttgac
     1921 cgacttcagc tccggatccg acaggttgcg ctcctggtag gcggcagcaa accccgccgc
     1981 atgcagcatc acattgcgct ggcgcagcgg gatatcgagc acgatcccca gcttgatgat
     2041 cagctccctg ctcggctgcg cgcgcccgct ttcgaggaag ctgatgtggc gctgcgagat
     2101 gccgctctcg gtcgccagcc gcagctggct gaagccgcgc ttgctgcgcc agtagcgcag
     2161 cgcgctggca aagtcgctga aataccggga tgcacttggg gcggacaggc ggggctcgtt
     2221 catcggacac ctttcaggcg ggctggaatg aacggattgt agggccgccg accgtggggg
     2281 cgtcgattac ctgccaggta attggcgcga cgcaccgatg ggccgacact gccgtcaacc
     2341 gcattccgtt atcccaaccc aggagaactt caatgagcgc ctacgccgtc ggacatctcg
     2401 aacacgtgaa catgggccag cccatcgtcg actatctgcg caacatcgac gcgacgctgg
     2461 cggagtacgg cggccgcttc atcatccatg gcgggcgcac cgacgtgctg gaaggaagct
     2521 tccggggcga cctgatcgtg atcgagttcc ctgacctggc ccgcgccagg gcctggtacg
     2581 cgtcacccgc ctatcgggaa atcctgccgc tgcgcacgga gaatgccagc ggtgcggtgt
     2641 tcctcatcga gggtgtcggc ccggaccaca aggcaacaga cgtgctcggc tgatcactgc
     2701 gccgggcgca gcatctggat caacggcggc gcctctgcca cctcgatggt gccgacgacc
     2761 cgaaaaccgt ggctttcata gagccgggtg taatccgggt tggtgttttc caagtacgcg
     2821 atcttgccct cgtcgtcgac gcgctcgagt ccgtggtgca gcagcgcgtg gccgtgtccg
     2881 cgtccctggt acgccgggtc gacgccgatg acgggcagat accagtgggg ctcctcgggc
     2941 cggctgctgc ccatttcgtc gaagaccgcc atcagcgttg gaagccggtc cggtccgacg
     3001 ctgcttctga gcacgtcgag gatctgctgt ttgtccggct cgattcctgg cggcagccac
     3061 aacgcggcgc cggcaaagcc ggcaatg
//
LOCUS       DATLGF010000488         7846 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_235444, whole genome shotgun sequence.
ACCESSION   DATLGF010000488 DATLGF010000000
VERSION     DATLGF010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 7846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7846
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235444"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="VM406_15170"
     CDS             complement(<1..1153)
                     /locus_tag="VM406_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVL77353.1"
                     /translation="MLPEKRLQQFLRAPGGRAGLALGTAAAAALVAAPWLPAWLLIPA
                     ALGLGIAIWLAVTSQSLHTDTALRESERKFRGFVEQSLVGLYMVQDGRIVYANPKTAE
                     LVGYETPEQLLGMPVEALTAPEDVPLLLENHRRRLSGEVPSVRYAYRALRRDGRRVWV
                     QVHGSACEYRGRPAVMGIGLDVSQQVEFERQSRLANRVFESASEGIVITDAEYRIEAV
                     NPAFTRITGYEVGEAVGKVSRMLTGQGAYSNQEMLARLTTDGRWEGEMSDRRKNGEWY
                     PAWLSISTVRDADGHLTNYVGVFTDNTRRKEAETRLQFLANHDTLTSMLNRSGMMLRF
                     EEVIARARRDGRRLALLFVDLDRFKTVNDTLGHTAGDRLIAVAAERIRAE"
     gene            1407..3881
                     /locus_tag="VM406_15175"
     CDS             1407..3881
                     /locus_tag="VM406_15175"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVL77354.1"
                     /translation="MNGPAHPRIEAVSESEALFRAMLDTLEEAYYEVDLAGNIVRCNA
                     AFYRMVGYTESELVGRNNQQFQTPEGASATYRMFNQVYKTGVPLTRCDVAFLHKDGRT
                     VLVEGSIHLTHDDQGRPNGFRGFQRDVTAQRRTETALRESEAKYRGILETIEDAYYEV
                     DLAGVMVLCNGAFCRMLGYASDDIIGRSYRTFQMPEAAASVYTTFNTVFRTGIGRRSF
                     DWTMIRRNGEPVTGEGSVQLVRDLNGRACGFRGILRDVTERRRVEQALRESEARFRAL
                     TKLSSDWYWEQDADFRYTRMEGRQDNRGGEDGLRAGFVGKRPWETALEIENEDGWDGH
                     RALLASRRPFRDVVMHRLLGDGRPYYISVSGEPFFTADGSFAGYRGVSRDITDRKLAE
                     ERIHHLATHDALTGLPNRSMFSQLLGAALEHARPAGSRFAVLFVDLDRFKHINDTLGH
                     DAGDRLLKKVTERFRVALRSSDVMARLGGDEFVILVRDLASTRHAGTVARKILAAAVE
                     PVMLNGRECRVSASIGIAIYPRDGSDEQTLMKNADIAMYYAKEEGKNNFQFYTEEIKS
                     LSLERLTLENHLRRALERGELSLHYQPKLHLASSAVAGVEALLRWNNAELGPIPPNVF
                     IPIAEETGLIIEIGKWVLREACEQNIAWQRAGLPPLSIAVNLSVRQFGDERLLADIAA
                     ILRDTGMSPQLLELEITEGMMINNPAHAARLLGGIRQLGVRLAIDDFGTGYSSLGQLK
                     NYPIDTLKIDRSFIRDIAVSAQDRAITEAIIAMGKSLGLSVVAEGVETPEQERFLRER
                     GCDEMQGFHFAHPMPGAQLEAFLRSA"
     gene            complement(3873..4280)
                     /locus_tag="VM406_15180"
     CDS             complement(3873..4280)
                     /locus_tag="VM406_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL77355.1"
                     /translation="MIKVVVIDANAISRNLLTSVLVNGGFEVVGDTNVSSAGLANAIK
                     LAPQLVCMDVGAIDEEGLARLDTIRQGLPKALVFLVSGKFDATGVQSARERGVHGFIV
                     KPFKADTVLATIRKAVITLARAHRHGRAEDHSG"
     gene            4453..4908
                     /locus_tag="VM406_15185"
     CDS             4453..4908
                     /locus_tag="VM406_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVL77356.1"
                     /translation="MYRKILVAYNGTPESRFALEECIRLNPGAAAQIHLLAVATPMPI
                     VLAGEFVAAVPTADEEDAERKAMAEVLESGRRLLAEAGLPVITHLEVGEPVNVISDLV
                     EQFNIELVIVGHSRHKPLALRWWRGSTDSVLVEKVRCSVLVAADPKRGE"
     gene            complement(4929..6035)
                     /locus_tag="VM406_15190"
     CDS             complement(4929..6035)
                     /locus_tag="VM406_15190"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HVL77357.1"
                     /translation="MTGPTRRLAPLALACSLAAAAEARVVPQTLASDLEHPWALAFIG
                     DGRMLVTERPGRLRVIEADGRVGEPVQGLPQIDAGGQGGLLDIVADRRFADNRTVYFC
                     YSEPGEGGNSTALASARLSQDRARLEQVRVLFSQRPKVRGSHHFGCRIVEAADGTLFL
                     TTGDRFNRMDDAQTLDNHLGKVVRVNKDGSVPKDNPFVQRAGALPEIWSYGHRNMQGA
                     TLGPDGRLWTHEHGPRGGDELNITQPGRNYGWPLVSHGVHYSGQEVGRGKAGQAGVEA
                     PVHHWTPSIAPSGMAFLDSDAYGQEWRGNLFVGALAGRHLVRLTVADGRVVAEEQLLK
                     DFGRRIRDVRMGPDGLLYLLTDERRGQLVRLRPE"
     gene            complement(6100..6426)
                     /locus_tag="VM406_15195"
     CDS             complement(6100..6426)
                     /locus_tag="VM406_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77358.1"
                     /translation="MANLRGILAGFGLERSGMHAGSDDHHDDNWITRPRFEPVEGGHY
                     LLAFSRIPAQGEPPVIKRIRVDGPTVEDWIDLDEQRPLDRALYAYAVKAWRPLKEDDR
                     QTGSLH"
     gene            complement(6485..6838)
                     /locus_tag="VM406_15200"
     CDS             complement(6485..6838)
                     /locus_tag="VM406_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77359.1"
                     /translation="MKHLGVLLAGLLALSACEAQVRNAPGVEERHSAPAQPDQPAAPD
                     ASGGDAAPAGPDEEREPGIAVAPPDTGVEEEMAVQPPPAGTGDDMITPAPPVEPGTMD
                     PEPPPGVAAAREQRK"
     gene            complement(6838..7338)
                     /locus_tag="VM406_15205"
     CDS             complement(6838..7338)
                     /locus_tag="VM406_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HVL77360.1"
                     /translation="MTENAPDAVELLTRQHREMERLLEEICQADEAGRKPLFATVADQ
                     LTIHIKAEEQIFYPAVHMARTEDDLLEALEEHLSLKRLLADLVELEPDDRAFEPKFKV
                     LKEQTEHHHREEEEHLFPAVLKLVGEQERCALGAQMLVLQAQLTRQDAPRCAVINETE
                     SAAPLD"
     gene            7450..>7846
                     /locus_tag="VM406_15210"
     CDS             7450..>7846
                     /locus_tag="VM406_15210"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HVL77361.1"
                     /translation="MKLKNAGHFFAARLPKCLVAHDVRQVKTFFREQAMLLRLGRLLR
                     MTGRDIVVLWYACRSPAAPRTLKLAAVLIALYVLSPIDLVPDWLPLLGWADDVTLLAL
                     ALPALLALFPPDALIDARAAAARWTWRTPW"
BASE COUNT         1267 a         2585 c         2639 g         1355 t
ORIGIN      
        1 gctcggcgcg gatgcgctcg gcagccaccg cgatcaaccg gtcgcccgcc gtatggccaa
       61 gcgtgtcgtt gacggtcttg aaacggtcca ggtcgacgaa cagcagcgcg agccggcgtc
      121 cgtcgcggcg cgcgcgcgca atcacctcct cgaaacgcag catcatgccg ctgcggttga
      181 gcatcgaggt gagggtgtcg tgattggcca ggaactgcag ccgggtctcg gcttccttgc
      241 ggcgggtgtt gtcggtgaac acgccgacat aattcgtgag gtgtccatcg gcgtcgcgca
      301 ccgtggagat cgacagccag gccggatacc attcgccgtt cttgcgccgg tcgctcatct
      361 cgccttccca gcgcccgtcc gtggtcaggc gcgcgagcat ctcctgattg ctgtacgcgc
      421 cttgcccggt cagcatgcgc gagaccttgc ccaccgcttc accaacctca tagccggtga
      481 tgcgcgtgaa ggcggggttg accgcctcga tgcggtactc ggcatcggtg atgacgatgc
      541 cctcgctggc cgattcgaac acccggttgg ccaggcgcga ctggcgctcg aattcgacct
      601 gctggctgac atcgagcccg attcccatca ccgccggccg gccccggtat tcgcatgcgc
      661 tgccatgcac ctgcacccag acgcgccggc cgtcgcggcg cagcgcccgg taggcgtagc
      721 gcacgctggg cacttcgccg gacaggcgcc ggcgatggtt ctccagcagc agcggcacgt
      781 cttccggcgc ggtcagcgcc tcgaccggca tgccgagcag ctgctccggc gtttcatagc
      841 cgaccagttc ggcggtcttc ggattggcgt agacgattct gccgtcctgc accatgtaca
      901 ggcccaccag cgattgttcg acgaaaccgc ggaacttgcg ctcggattcg cgcaatgcag
      961 tgtcggtgtg cagggactgg ctcgtcaccg ccagccagat cgcgatgccc aggcccaggg
     1021 cggccggaat cagcagccag gccggcagcc aaggtgcggc gacgagcgcc gccgccgctg
     1081 ccgtgccaag cgccaggccg gcgcgcccgc caggcgcacg caggaattgt tgtagtcgct
     1141 tttctggcaa cactggatct atcaaggagc gagcgacggc agctgccggc ccggtgtgaa
     1201 tcttcaacaa gttctgttcg gcaacatggt agccgtacgg gtaccatccg gcaatggcag
     1261 tgtggcgcgg tggcagcgcc gcctgtgcat tccgccggca cggtgcaata atcgccggaa
     1321 actacgactg ccatgacggc cgacatgccc ccttactccc cttccgatcg tctcgctggc
     1381 cgggctgccg gcgcccggca ggcatcatga acggccccgc gcacccgagg atcgaggcgg
     1441 tgagcgaaag cgaggcactg tttcgcgcga tgctcgatac cctggaagag gcgtactacg
     1501 aagtcgacct ggccgggaac atcgtgcgct gcaatgccgc gttctaccgg atggtcggct
     1561 ataccgagtc ggaactggtc ggtcgcaaca accagcagtt ccagaccccg gaaggtgcga
     1621 gcgccaccta ccggatgttc aaccaggtct acaagaccgg ggtgccgctg acccgctgcg
     1681 acgtcgcgtt tctccacaag gacggcagga ccgtgctggt cgaggggtcg atccacctga
     1741 cccatgacga ccagggacgc ccgaacggct ttcggggctt ccagcgcgat gtcacggcgc
     1801 agcgccgcac cgagacggcc ctgcgcgaaa gcgaggcgaa gtatcgcggc atcctggaga
     1861 cgatcgagga cgcctactac gaggtcgatc tcgccggcgt gatggtgctg tgcaacggtg
     1921 cgttctgccg catgctcggc tacgcgagcg acgacatcat cggccgcagc tatcggacct
     1981 tccagatgcc cgaggccgcg gcaagcgtat acaccacctt caacaccgtc tttcgcaccg
     2041 ggatcggcag gcgcagcttc gactggacga tgatccgcag gaatggcgag ccggtgaccg
     2101 gagaagggtc ggtgcagctg gtgcgcgatc tcaacggacg cgcctgcggc tttcggggca
     2161 tcctgcgcga cgtgaccgag cggcgccgcg tggagcaggc gctgcgcgag tcggaggcgc
     2221 gttttcgcgc gctgaccaag ctctccagcg actggtactg ggagcaggat gcggacttcc
     2281 gctatacccg catggagggg cggcaggaca accgcggagg ggaagacggc ctgcgcgccg
     2341 gctttgtcgg caagcgaccc tgggagacgg cgctcgaaat cgagaacgag gacggctggg
     2401 acggccaccg tgcgctgctg gcctcgcgcc ggccgtttcg cgacgtcgtg atgcaccggc
     2461 tgctgggcga cgggcggccc tactacatca gcgtcagcgg cgagccgttc ttcacggccg
     2521 acggcagctt cgccggctat cgcggggtgt cgcgcgacat caccgaccgc aagctggccg
     2581 aggagcgcat tcaccatctc gccacccacg acgcgctgac cggactgccg aaccggtcga
     2641 tgttcagcca gctgctgggc gcggcgctgg agcacgcgcg tccggcgggc agccgcttcg
     2701 cggtgctgtt tgtcgacctc gatcgcttca agcacatcaa tgacaccctc ggtcatgacg
     2761 ccggggaccg gctgctgaaa aaggtcaccg agcggttccg tgtggccctg cgctccagcg
     2821 acgtgatggc ccgtctcggc ggggacgagt tcgtgatcct ggtgcgcgat ctcgccagta
     2881 cccggcatgc gggcacggtg gcccgcaaga tcctggccgc cgcggtcgag ccggtgatgc
     2941 tcaacgggcg ggagtgccgt gtatcggcaa gcatcggcat cgcgatctat ccgcgggacg
     3001 gttccgacga acagacgctg atgaagaacg ccgatatcgc gatgtactac gcgaaggagg
     3061 agggcaagaa caacttccag ttctatacgg aggagatcaa gtcgctgtcg ctggagcggc
     3121 tgacgctgga gaatcacctg cgccgggcgc tcgagcgcgg cgagctgtca ctgcactatc
     3181 agcccaagct gcatctggcc agctcggcgg tggctggcgt ggaggcgctg ttgcgctgga
     3241 ataatgcgga gctgggaccg attccgccca atgtcttcat accgatcgcc gaggagaccg
     3301 gtctcatcat cgagatcggc aaatgggtgc tgcgcgaggc ctgcgaacag aacatcgcct
     3361 ggcagcgcgc ggggctgccg cccttgtcga tcgcggtaaa cctgtcggtg cgccagttcg
     3421 gcgacgagcg cctgctggcg gatatcgccg ccattctccg ggacaccggc atgtcgccgc
     3481 agttgctgga actcgagatc accgaaggca tgatgatcaa caacccggcg catgcggcgc
     3541 ggctcctggg cgggatcagg cagctcggcg tgcgcctggc gatcgacgat ttcggaaccg
     3601 gctattcgtc attgggccag ttgaagaact atccgatcga cacgctgaag atcgaccgct
     3661 cgttcattcg cgatatcgcc gtcagcgcgc aggacagggc gatcaccgaa gccatcatcg
     3721 cgatgggcaa gtcgctcggc ctgtcggtcg ttgccgaagg ggtggagaca ccggaacagg
     3781 agcgtttcct gcgcgagcgc ggatgcgacg agatgcaggg gtttcacttc gcccacccga
     3841 tgccgggcgc gcagctcgag gcatttctgc gctcagcctg aatggtcttc cgcgcgcccg
     3901 tgccggtgcg cgcgcgcaag cgtgatgacc gccttgcgta tcgtcgccag cacggtgtcg
     3961 gccttgaagg gcttgacgat gaagccgtgc accccgcgct cgcgcgcgct ctgcactccg
     4021 gtcgcgtcga acttgccgga gaccaggaac acgagtgcct tgggcaggcc ctggcggatc
     4081 gtatcgagcc gcgccagccc ctcctcgtcg atcgcgccca cgtccatgca caccagttgc
     4141 ggtgcgagct tgatcgcatt ggccaggccg gccgaggaca cattggtgtc gcccacgacc
     4201 tcgaagccgc cattgaccag cacggaagtc agcaggttgc gggaaatggc attggcatcg
     4261 atgacgacga ccttgatcat cggggcggct ccggaatttt ttgcgctcat tatccgcgag
     4321 cggcagacat tgcgccagcg caacctcgcg cgctatagtg cggccgcgcc cgtcggattc
     4381 cgcgtggcac gacgtgcgat ccactagaat gaaaccggat gttcaaccgc gcgcacgcgc
     4441 aaggagactg gcatgtaccg caaaatcctg gtcgcctaca acggcacacc cgagagccgc
     4501 ttcgctctgg aagagtgcat caggctcaat cccggcgctg ccgcgcaaat ccatctgctc
     4561 gcggtggcca cgccgatgcc catcgtgctc gccggcgaat tcgttgccgc cgtcccgacg
     4621 gccgacgagg aggatgccga gcgcaaggcc atggccgagg tgctcgagtc gggccgcagg
     4681 ctgctggcgg aggcgggcct gcctgtgatc acgcatctgg aggtcggcga gccggtcaac
     4741 gtgatctccg acctggtcga gcagttcaac atcgaactcg tgatcgtcgg ccattcccgc
     4801 cacaagccgc tggcgctgcg ctggtggcgc ggttcgaccg actcggtgct ggtcgagaag
     4861 gtgcgctgca gcgtgctggt ggcggccgat ccgaagcgcg gcgaataagc cgaagcgcag
     4921 cgaataaact attcgggccg cagccgtacc agctgaccgc ggcgctcgtc ggtcagcagg
     4981 tagagcaacc cgtccggacc catacgcacg tcgcggatgc gccggccgaa atctttcagc
     5041 agctgttcct cggccaccac gcggccgtcg gcgacggtga gccgcaccag atggcggccg
     5101 gcgagcgcgc cgacgaacag gttgccgcgc cattcctggc cgtaggcgtc gctgtccaga
     5161 aaggccatgc cggaaggtgc gatcgacggg gtccagtggt ggaccggcgc ctccacaccg
     5221 gcttgcccgg ccttgccgcg acccacctct tgtccactgt aatgcacgcc gtgactgacc
     5281 agcggccagc cgtaattgcg gccgggctgc gtaatgttca gttcgtcgcc gccgcgcggg
     5341 ccgtgttcat gcgtccacag gcgcccgtcg gggccgagcg ttgcgccctg catgttgcgg
     5401 tgaccgtagc tccagatttc cggcagcgcc cccgcgcgct gcacgaacgg attgtccttc
     5461 gggaccgaac cgtccttgtt gacgcggacc accttgccga gatggttgtc cagcgtctgg
     5521 gcatcgtcca tccggttgaa gcggtcgccc gtggtcagga acagcgtgcc gtcggccgcc
     5581 tccacgatgc gacagccgaa gtggtggctg ccgcgcacct tcggccgctg cgagaacagc
     5641 acgcgcacct gctcgagtcg tgcgcggtcc tgcgacaggc gggccgacgc aagcgcggtc
     5701 gagttgccgc cttcgccggg ttcggagtag cagaagtaga cagtgcggtt gtccgcgaaa
     5761 cggcggtcgg cgacgatatc gagcaggccc ccctgtccgc cggcgtcgat ctgcggcagc
     5821 ccctgcaccg gctcgccgac gcgcccgtcg gcctcgatca cgcgcaggcg gcccgggcgc
     5881 tcggtgacga gcatgcggcc gtcgccgatg aaggccagcg cccacggatg ctcgaggtcg
     5941 gacgcaagcg tctgcggtac gacacgtgct tcggcggccg cggccaggga acaggcgagg
     6001 gcaagtgggg ccaggcggcg cgttgggccg gtcatcggag gtcctcccat tcttggaatt
     6061 gtgaaatgcg gcgttgcgaa ggcgcaggcc ctcgcgtgct caatgcaggc tgccggtctg
     6121 cctatcatcc tccttcagcg gccgccatgc cttgaccgca tacgcataca gcgcgcggtc
     6181 cagcggacgc tgctcgtcga ggtcgatcca gtcctcgacg gtggggccgt cgacccggat
     6241 acgttttatc acaggcggtt cgccctgcgc cgggatgcgc gagaacgcga gcaggtaatg
     6301 tccgccttcg acgggctcga agcgggggcg ggtgatccag ttgtcgtcat ggtgatcgtc
     6361 gcttccagcg tgcatgccgg atcgttcaag gccgaatccg gccagaatgc cgcgcaggtt
     6421 cgccatgctc agtcctccta ggtcagtgcc ggtggtgaaa atcgcgaggc aacgcggggg
     6481 cgggttattt gcgttgctcg cgagcggccg ccacgccggg cggcggttcc ggatccatcg
     6541 tccccggctc gaccgggggc gcgggagtga tcatgtcgtc gccggtgccg gccggaggcg
     6601 gctgtaccgc catctcctcc tcgacaccgg tgtccggggg cgcgaccgca atgccgggtt
     6661 cgcgctcttc atccgggccc gcaggggcgg cgtcgccgcc cgaggcgtct ggcgccgccg
     6721 gctggtccgg ctgcgcgggc gcgctgtgcc gctcctcgac gccgggcgcg ttcctgacct
     6781 gcgcctcgca agccgagagc gccagcaatc ccgccagcag cacgcccagg tgtttcatca
     6841 gtccagcgga gcggccgact ccgtctcgtt gatgacggcg cagcgcggcg cgtcctgccg
     6901 cgtcaactgc gcctgcagga caagcatctg cgcgcccagc gcgcagcgct cctgttctcc
     6961 gaccagcttg agcacggccg gaaacaagtg ttcttcctcc tcgcgatggt gatgctcggt
     7021 ctgttccttg agcaccttga atttcggttc gaatgcccga tcgtccggtt ccagttcaac
     7081 caggtcggcc agcagccgct tgagcgacag atgttcctcc agcgcttcga gcaggtcatc
     7141 ctcggtgcgg gccatgtgca cggccgggta gaaaatctgt tcctcggcct tgatgtgaat
     7201 ggtgagctga tcggccactg tcgcaaacag cggcttgcgc ccggcctcgt cggcctggca
     7261 gatttcttcc agcaggcgct ccatctcgcg gtgctggcgc gtgagcagtt ccaccgcgtc
     7321 gggtgcgttc tcggtcatgc gcagtctctc cgttgaagaa ttgtcaccgg ttagagaggg
     7381 cgactgccca ctggttcagg ccttgcggaa ctgttacatc ggcgtcgatt tccttgcggg
     7441 aaacgtttga tgaaactcaa aaatgccggg cactttttcg ccgctcgcct gcctaaatgc
     7501 cttgtcgcgc atgacgtgcg gcaagtaaag acttttttca gggagcaagc gatgctgtta
     7561 cgacttggcc gcctgttgcg gatgacgggg cgcgatatcg tggttctctg gtacgcctgc
     7621 cggagtccgg cggcgccgcg gaccctgaag ctggcggcgg tgctcattgc cttgtacgtg
     7681 ctcagcccga tcgatctggt acccgattgg ttgccccttt tgggctgggc cgacgacgtc
     7741 accctgcttg ccctcgcatt gccggccctg ctggcgctgt ttccgcccga tgcgctgatc
     7801 gatgcgcgtg cggcggcggc gcgctggacc tggcgcaccc cctggc
//
LOCUS       DATLGF010000489         2730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_78724,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000489 DATLGF010000000
VERSION     DATLGF010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78724"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VM406_15215"
     CDS             <1..599
                     /locus_tag="VM406_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL77362.1"
                     /translation="YEVQTVVNDLSKAGAAAALVDAVVERFGTIDILVNNAGATWSAP
                     AETYPDEAWRKVMGLNVDALFFLAREVANRCMIPRRHGKIINIASVAGLRGSATNVTT
                     IAYHTSKGAAVNFTRALASEWGKYNINVNAICPGFFPSKMSAGLLERVADAVIARAPL
                     HRIGGDEDLKGCVVFLASEASRHITGQAIAVDGGISAV"
     gene            654..1871
                     /locus_tag="VM406_15220"
     CDS             654..1871
                     /locus_tag="VM406_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVL77363.1"
                     /translation="MPFTFSEKSVELQQRVKAFIDSEIIPAEPAYKAEMAAARRAGNP
                     WIVPQVVEDLKARAQAQGLWNLFLPESKYGAGLSNLDYAPLCELMGRVSIADEVFNCS
                     APDTGNMETLVRYGSPEQQEQWLLPLLEGRIRSGFAMTEPAVASSDATNIEARIERDS
                     NEYVINGRKWFTSGASDPRCKILIFMGKTDPSNPNRHRQQSMILVPTDTPGVQIERNL
                     SVFGFDDAPHGHAEVSFENVRVPATNILLGEGRGFEIAQGRLGPGRIHHCMRLIGRAE
                     VALEMMCKRTLARTAFGRRIADQGVWRERIANARILIDQARLMVLNAADMMDTVGNKA
                     AAKEIAMIKVIAPNMACQVIDWAIQAHGGGGVSQDFPLAQSYASARSIRLADGPDEVH
                     RNAIAKLELARYQ"
     gene            1887..>2730
                     /locus_tag="VM406_15225"
     CDS             1887..>2730
                     /locus_tag="VM406_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVL77364.1"
                     /translation="MSDRHFAHWPQNQSHELTVPATNLFYNVEVSAARYPNKPYILFY
                     DTPISFSEFKDEAERIAGYLVQVCRVRRGDRVLLFMQNSPQFALAYYGILRANAVVVP
                     VNPMNLTNELRHYVQDTGATAAIVAQDLYAQMAPLMEEGLQNILVAAYSDYLKRPTDL
                     RVPEFVTAPRQRIEDKGVTLWADALAMNLKPGPLTAGPDDLCVMPYTSGTTGNPKGCM
                     HTHRSAMANAVASMQWFSSTPDSVGLAVLPFFHVTGMQGSMSGPLFVGSTIVLLPTNS
                     GPLML"
BASE COUNT          514 a          920 c          862 g          434 t
ORIGIN      
        1 gctatgaagt gcagaccgtc gtcaacgacc tctcgaaagc aggcgccgcg gcggcgctgg
       61 tcgacgcggt ggtcgagcga ttcggcacca tcgacatcct ggtcaacaac gccggcgcca
      121 cctggagcgc accggccgaa acctatcccg acgaagcctg gcgcaaggtg atgggcctga
      181 acgtggacgc cctcttcttc ctggcacgcg aagtcgccaa ccgctgcatg attccgcgcc
      241 gccacggcaa gatcatcaac atcgcgtcgg tggcgggcct gcgcggcagt gcgaccaacg
      301 tcaccaccat cgcctaccac acgtccaagg gcgcggccgt caacttcacg cgcgcgctgg
      361 cctccgaatg gggcaagtac aacatcaacg tcaacgcgat ctgtccgggc ttctttccat
      421 cgaagatgtc ggccggcctg ctcgaacggg tggccgacgc ggtcatcgcg cgcgcaccgt
      481 tgcaccggat cggcggcgac gaggacctga aaggctgcgt ggtattcctc gcctcggagg
      541 cgtcgcgcca catcaccggg caggcgatcg cggtcgacgg cggcatcagt gcggtctgac
      601 aaccccgtct tgcgaacccg ccggccctgc ggccggcctt acacggagca tccatgccat
      661 tcacgttttc cgagaagtcc gtcgaactgc aacagcgcgt caaggcgttc atcgacagcg
      721 aaatcatccc ggccgaaccc gcgtacaagg cggaaatggc ggcagcccgc cgcgccggca
      781 atccgtggat cgtgccgcag gtggtggagg atctgaaggc ccgcgcgcaa gcccagggac
      841 tgtggaacct gttcctgcca gagtccaaat atggcgccgg cctcagcaac ctcgactatg
      901 cgccgctgtg cgaactgatg ggccgggtat cgatcgcgga cgaggtgttc aactgctcgg
      961 cgccggatac cgggaacatg gaaaccctgg tgcgctacgg ctcgccggag cagcaggagc
     1021 aatggctgct gccgctgctc gaaggccgca tccgctccgg cttcgcgatg accgagccgg
     1081 cggttgcgtc gtcggacgcg accaatatcg aggcgcgcat cgagcgcgac agcaatgagt
     1141 atgtgatcaa cgggcgcaag tggttcacct cgggagcaag cgatccgcgc tgcaagatcc
     1201 tgatcttcat gggcaagacc gacccgagca atccgaaccg gcatcgccag caatcgatga
     1261 tcctggtgcc gaccgacacg ccgggcgtgc agatcgagcg caatctctcg gtgttcggtt
     1321 tcgacgacgc cccgcacggc catgcggagg tcagtttcga gaacgtgcgc gtgccggcca
     1381 ccaacatcct gctcggagag gggcgcggct tcgagatcgc gcagggccgc ctcggccccg
     1441 gccgcatcca tcactgcatg cgcctgatcg ggcgcgccga agtcgcgctt gagatgatgt
     1501 gcaagcgcac gctggcgcgc actgcgttcg ggcgcaggat cgccgatcag ggcgtctggc
     1561 gcgaacgaat tgcgaacgcg cgtatcctga tcgaccaggc gcgcctgatg gtgctcaacg
     1621 ccgccgacat gatggacacc gtgggcaaca aggccgcagc caaggaaatc gcgatgatca
     1681 aggtgatcgc gccgaacatg gcctgccagg tgatcgactg ggcgatccag gcccatggcg
     1741 gcggcggcgt gagccaggat tttccgctgg cgcagtccta tgcgagcgca cgctcgatcc
     1801 ggctggccga cggccccgat gaagtccacc gcaatgccat cgccaagctg gaactggcgc
     1861 gttatcaata accaccaccc actgccatgt ccgatcgcca tttcgcccac tggccgcaga
     1921 accagtcgca tgaactgacc gtgcccgcga ccaacctgtt ctacaacgtc gaggtttcgg
     1981 ccgcgcgcta cccgaacaag ccctacatcc tgttctatga cacgccgatc agcttttccg
     2041 agttcaagga cgaggccgag cgcattgccg gctatctcgt ccaggtatgc cgggtgcggc
     2101 gcggcgacag ggtcctgctg ttcatgcaga acagcccgca gttcgcgctt gcctactacg
     2161 gcatcctgcg cgccaatgcg gtggtggtgc cggtcaaccc gatgaacctg accaacgagc
     2221 tgcgccacta tgtgcaggac accggcgcaa ctgccgccat cgtcgcacag gacctgtatg
     2281 cgcagatggc gccgctgatg gaagaaggcc tgcagaacat cctggttgcc gcctactccg
     2341 actacctgaa gcgccccacc gacctgcgcg tgccggaatt cgtgaccgcg ccacggcaac
     2401 ggatcgagga caagggcgtc accctgtggg cggacgcgct ggcgatgaac ctgaagcccg
     2461 gtccgctcac cgccggaccg gacgacctgt gcgtgatgcc ctatacctcc ggcaccaccg
     2521 gcaatccgaa gggctgcatg cacacccatc gcagcgcgat ggccaacgcg gtggcgagca
     2581 tgcaatggtt ctcgtcgacg ccggacagcg tcggccttgc ggtgctgccg ttcttccatg
     2641 tgaccggcat gcagggcagc atgagcggcc cgctgttcgt cggcagcacc attgtgctgc
     2701 tgccgacgaa cagcgggccg ctcatgctgt
//
LOCUS       DATLGF010000490         5455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_202872, whole genome shotgun sequence.
ACCESSION   DATLGF010000490 DATLGF010000000
VERSION     DATLGF010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202872"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VM406_15230"
     CDS             <1..785
                     /locus_tag="VM406_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HVL77365.1"
                     /translation="ITVQLEAPGVGENLQDHLQLRMAYKVEGVRTLNTMANSLLGKMR
                     IGMQYMLTRSGPMSMAPSQLGAFTRSDPAQATPNLEYHVQPLSLDKFGEPLHAFPAFT
                     ASVCNLRPTSRGHVRLTSADPTVAPRIAPNYLSTSEDRKVAADALKLTRRIAAAAPLQ
                     KYRPEEFRPGVHYRTEEELAKAAGDIGTTIFHPVGTCKMGKADDPLAVVDSRLRVKGV
                     GRLRVVDASVMPTITSGNTNSPTLMIAEQAARWIREAYAKNA"
     gene            complement(822..1994)
                     /locus_tag="VM406_15235"
     CDS             complement(822..1994)
                     /locus_tag="VM406_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HVL77366.1"
                     /translation="METKAFNPFTFSTVPNIVCEAGSSARIGSLLRQQFPGAKRALVV
                     TDAGFLRTGLLDAPLQALRDADFNPQIFSNVVADPPEAVVLEAVDEARAKQIDLVIGW
                     GGGSSMDVAKLIAVLVPSNQELKTMYGIGNVKGERLPLVQIPTTAGTGSEVTPISIVT
                     TGETTKMGVVSPQLYADLAVLDASLTVGLPSKVTAATGIDAMVHAIEAYTTKHKKNAL
                     SDMLARQALSLLSNNILQACRNGGDLAAREAMLLGATLAGQAFANAPCAAVHALAYPI
                     GGIFHVPHGLSNSLVLPHVLRFNAPEAARQYAELAEIVAPQARGSDEARTEALIARMD
                     EIARETGIETRLREVGIAETDLDRLADDAMLQTRLLTNNPRAVLRADARAIYAAAL"
     gene            2106..2771
                     /locus_tag="VM406_15240"
     CDS             2106..2771
                     /locus_tag="VM406_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77367.1"
                     /translation="MFSETTTFQRTHTGRTEIHEKKSGLTQSERLVLIMIDGVTPFSG
                     VRAKLPVLTEDRFARAVQTLLNKDLILEVILPVEGQAPELVERTIIDRFLQQDPLDPV
                     TIMYREDDERFAGYIPTQQKTAGAASAARVEQAPDVQPYHNRVPAPNLLDDRDEEAAE
                     EIGAAARAKALEHPLNSIQFIPRRVEKSPTGAKAEATGWQYWMLAVIGAFILGYAFAR
                     LSI"
     gene            2899..4833
                     /gene="mnmG"
                     /locus_tag="VM406_15245"
     CDS             2899..4833
                     /gene="mnmG"
                     /locus_tag="VM406_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HVL77368.1"
                     /translation="MLFPSEFDVIVVGGGHAGTEAALASARMGQKTLLLTHNIETLGQ
                     MSCNPSIGGIGKGHLVKEVDALGGAMAIATDEGGIQFRILNSSKGPAVRATRAQADRI
                     LYKAAIRRRLENQPNLWLFQQAVDDLMVEGDRVVGAVTQVGVRFRSRAVVLTAGTFLD
                     GKIHVGLNNYSAGRAGDPPAVSLSARLKELKLPQGRLKTGTPPRIDGRSIDFSVMEEQ
                     PGDLDPIPVFSVMGNAAMHPQQMPCWITHTNDRTHEIIRGGLDRSPMYTGVIEGVGPR
                     YCPSIEDKIHRFADKGSHQIFLEPEGLTTNEFYPNGISTSLPFDVQLALVHSIRGLEN
                     AHILRPGYAIEYDYFDPRGLKASLETRAISGLFFAGQINGTTGYEEAAAQGLLAGINA
                     ALQTQGREPWTPRRDEAYLGVLVDDLITKGVQEPYRMFTSRAEYRLSLREDNADLRLT
                     EIGRKLGCVGDAQWEVFERKREAIARELERLKSTWVNPRLLPEAEAERVLGKAIEREY
                     ALTDLLRRPNVSYESLMTLKGADGQDLAGPGVADDAVREQVEIQVKYAGYIDRQAKEV
                     ERHDHYENLKLPAELDYMDVRGLSIEVRQKLNKHRPETLGQASRISGVTPAAISLLLV
                     HLKKGGFRDLLNGQDAQSAA"
     gene            4842..>5455
                     /gene="rsmG"
                     /locus_tag="VM406_15250"
     CDS             4842..>5455
                     /gene="rsmG"
                     /locus_tag="VM406_15250"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HVL77369.1"
                     /translation="MSSFDRAHLTERLRAGVDALQLQLDDGQIGKLIDYLGLLAKWNA
                     VYNLTAVRDPAQMVVQHLLDSLAAVPAFAGAHNVLDVGAGGGLPGIVLAIARPEMQLT
                     LVDTVHKKTAFLTQVKAELGLANVSVHHARVEQMQVAQKFDVITSRAFAELNDFVTWS
                     GHLLADGGCFIALKGVRPDDEVARLPAGWKLAKLVPLDVPGLGA"
BASE COUNT         1029 a         1795 c         1715 g          916 t
ORIGIN      
        1 ggatcacggt gcaactggag gcgcccggtg tcggtgaaaa cctccaggat cacctgcaac
       61 tgcgcatggc ctacaaggtc gagggcgtac gcacgctcaa tacgatggcc aacagcctgc
      121 tcgggaagat gcgcatcggc atgcagtaca tgctgacccg cagcggcccg atgtcaatgg
      181 cgccgtctca gctgggtgcg ttcacccgtt ccgacccggc ccaggccacg ccgaatctcg
      241 aataccatgt gcagccgctg tccctggaca agttcggcga gccgctgcat gcgttccccg
      301 ccttcacggc aagtgtctgc aatctgcggc cgacctctcg cggccatgtc cggctcacgt
      361 cggccgatcc gacggtcgcc ccgcgcatcg cgccgaatta cctgagtacg tcggaggacc
      421 gcaaggtcgc ggccgatgcg ctgaagctca cgcgccgcat cgcggccgcc gcgccgttgc
      481 agaagtaccg cccggaggag ttccggcccg gcgtgcacta ccggaccgag gaggagctgg
      541 ccaaggccgc cggcgacatt ggcaccacga tcttccatcc ggtcggcacc tgcaagatgg
      601 gcaaggcaga cgatccgctt gccgtggtgg acagtcgcct gcgggtgaag ggggtagggc
      661 gcctgcgcgt ggtcgacgcc tcggtgatgc cgaccattac ctccggcaac acgaactcac
      721 cgacactgat gatcgccgag caggcggcgc ggtggatccg tgaagcttat gcaaaaaacg
      781 catagttttg ccggcaacat atcgccccct aaagcgcgaa ttcagagtgc cgctgcatag
      841 atcgcacgcg catccgcacg cagcacggcg cgcgggttgt tcgtgagcaa gcgcgtctgc
      901 agcatcgcat catccgccag ccggtccaga tcggtttcgg cgatccctac ctcgcgcagc
      961 cgggtttcga tgcccgtttc ccgcgcgatc tcgtccatcc gggcgatcaa ggcctcggtc
     1021 ctcgcctcgt cgctgccgcg cgcctgcggc gcgacgatct ccgccagctc cgcgtattgc
     1081 ctcgccgcct cgggtgcgtt aaagcgcagc acgtgcggca ggaccagcga gttcgacaag
     1141 ccgtgcggaa cgtggaagat tccgccgatc ggataggcga gggcgtgtac ggcggcgcag
     1201 ggcgcgttcg cgaacgcctg gccggcgagc gtggcgccca gcaacatcgc ttcacgcgcg
     1261 gccaggtcgc caccgttacg gcatgcctga aggatattgt tcgaaagcaa tgagagggcc
     1321 tggcgcgcaa gcatgtccga gagcgcattc tttttgtgct tggtcgtata cgcctcgatt
     1381 gcatggacca tcgcatcaat gccggtggca gccgttacct tggacggcaa gccaacggtc
     1441 agactggcat cgagtaccgc caggtcggca tagagctgcg gcgagaccac gcccatcttc
     1501 gtcgtttcgc cggtcgtcac gatcgagata ggcgtcactt cggagcctgt gccggcggta
     1561 gtcggaatct gcacgagcgg cagacgctcc cccttcacat tcccgatgcc gtacatggtc
     1621 ttgagttcct ggttcgaggg caccagtacc gcgatcagct tggcgacgtc catcgagctg
     1681 ccgccacccc agccgatgac gaggtcgatc tgtttggcac gcgcctcgtc gacggcctcc
     1741 aggacgaccg cctcgggcgg atcggcgacg acgttcgaga atatctgggg attgaaatcg
     1801 gcgtcgcgca gcgcttgcag cggagcatcg agcaggccgg tccggagaaa gccggcgtcg
     1861 gtcacgacca gcgcccgctt cgcgccggga aactgttggc gcagcagact tccaatccgg
     1921 gcgctgcttc cggcttcaca gacgatgttg gggacggtgc tgaatgtgaa cggattgaaa
     1981 gcttttgttt ccattttctt ttctcctggc agttttgtct ccgggtccag ctggtattgt
     2041 aacgggcagg ggccgacttc aagcggccca gctcaatgtt ccacgtgaaa caatccgggg
     2101 ggccaatgtt cagcgaaacg acgactttcc agcgaaccca cactggtcgc acggaaatcc
     2161 atgaaaaaaa gagcgggctc acgcaaagcg agcgcctggt gctgatcatg atcgacggcg
     2221 tcacgccatt ttcaggtgtg cgagcgaaac tgcctgtgtt gacagaagat cgcttcgccc
     2281 gagccgtgca aaccctgctg aacaaggatc tgatcctgga agtgatcctg cctgtcgaag
     2341 ggcaggcccc ggaactcgtc gagcggacga tcatcgaccg attcctgcag caggacccgc
     2401 tggaccccgt cacgatcatg tatcgggagg acgatgagcg ctttgccggc tatattccca
     2461 cccagcaaaa gacagcgggg gccgcctcgg ccgcgcgggt cgaacaagca cccgatgtac
     2521 agccttatca caaccgggtg ccggcgccca atttgctgga cgacagggac gaagaagccg
     2581 ccgaagagat cggtgcggcg gcacgcgcca aggcccttga gcacccattg aattccattc
     2641 aattcatccc ccggcgcgtg gaaaaatcgc cgaccggggc gaaggccgag gccaccggat
     2701 ggcaatactg gatgctcgct gtcatcggcg cctttattct cggatacgcg ttcgcgcgcc
     2761 tcagcatcta gctacctcat gtttcacgtg aaacataccc gacccggcgt cgccggcact
     2821 cccggaaacg acttataatc tcgcctctga cgccttctcc gcgtcccgat ctttaccccg
     2881 tcgccccgag gcgccaccat gctattccct tcagagttcg atgtcatcgt agtcggcggc
     2941 ggtcatgccg gcacggaagc cgcccttgct tctgcccgga tgggccagaa aaccctgctg
     3001 ctcactcata acatcgagac tttgggccag atgtcatgca atccgtcgat cggcggcatc
     3061 ggcaagggac acctggtgaa ggaagtcgac gcgctcggcg gtgcgatggc gattgccacc
     3121 gacgaaggcg gcatccagtt ccgcatcctg aattcctcga agggccccgc cgtgcgtgcc
     3181 acccgcgcgc aggccgaccg catcctctac aaggcggcaa tccgccggcg cctcgaaaac
     3241 cagccgaatt tatggttgtt ccagcaggcg gtcgacgacc tgatggtcga gggcgaccgc
     3301 gtcgtcggcg cggtgaccca ggttggcgtg cgtttccgca gccgtgcggt ggtattgacc
     3361 gcaggaacct tccttgacgg aaagatccac gtcggcctga acaactactc ggccggccgc
     3421 gccggtgatc cgccagccgt gtcgctgtcg gcgcggctga aagaactgaa gctgccgcag
     3481 ggccggctga aaaccggcac gccaccgcgc atcgacggcc gcagcatcga tttctcggtg
     3541 atggaagaac agccgggcga tctggatccg atcccggtgt tctcggtgat gggcaatgct
     3601 gcgatgcatc cgcagcagat gccgtgctgg atcacccaca cgaacgatcg cacgcacgag
     3661 atcatccgcg gcggcctcga ccgcagcccg atgtacaccg gcgtgatcga aggggtgggg
     3721 ccgcgttact gcccgtcgat cgaagacaag atccaccgct tcgccgacaa gggttcgcac
     3781 cagatcttcc tcgaaccgga aggcctgacc acgaacgagt tctacccgaa cggcatctcg
     3841 accagcctgc ctttcgatgt acagctcgcg ctcgtgcatt cgatccgcgg cctcgagaac
     3901 gcgcacatcc tgcgcccggg ctatgcaatc gaatacgact acttcgaccc gcgcggcctg
     3961 aaggcttcgc tcgaaacccg ggcgatctcc ggcctgttct tcgccggcca gatcaacggc
     4021 accaccggct acgaggaagc cgcggcccag ggcctgctgg ccgggatcaa cgccgcgctg
     4081 cagacccagg gccgcgagcc ctggacgccg cgccgcgacg aagcctatct cggcgtgctg
     4141 gtcgacgacc tgatcaccaa gggcgtgcag gaaccgtacc ggatgttcac cagccgcgcc
     4201 gaataccgtc tctcgctgcg cgaggacaat gccgacctgc gcctcaccga aatcggccgc
     4261 aagctcggct gtgtcggcga cgcgcagtgg gaagtgttcg aacgcaagcg cgaagcgatc
     4321 gcgcgcgagc tcgaacgcct gaaatccacc tgggtcaatc cccgtctgct gccggaagcc
     4381 gaagccgaac gcgtgctcgg caaagcgatt gagcgagagt atgcgctcac cgacctgctg
     4441 cgccggccga acgtcagtta tgaaagcctg atgacgttga agggcgccga cggccaggac
     4501 ctcgccggtc ccggcgtggc ggacgatgcg gtgcgcgaac aggtcgagat ccaggtcaag
     4561 tacgccggct acatcgaccg ccaggccaag gaagtcgagc gccacgacca ctacgaaaac
     4621 ctgaagctgc cggccgagct cgactacatg gacgtgcgcg gcctgtcgat cgaggtgcgg
     4681 cagaagctga acaagcaccg gcccgagacg ctgggccagg cgtcgcggat atccggcgtg
     4741 acgccggcgg caatctcgct gctgctggtg cacctgaaga agggcggctt tcgcgatctg
     4801 ctgaacgggc aggacgccca gagcgcggcg taagcaaacc gatgtcatcc ttcgaccgag
     4861 cgcacctgac cgagcgcctg cgcgccggcg tcgacgcgct gcagctgcaa cttgatgacg
     4921 gccagatcgg aaagctgatc gactacctcg gcctgctcgc gaaatggaac gcggtctaca
     4981 acctgaccgc ggtgcgcgat ccggcccaga tggtggtgca gcacctgctc gattcgctcg
     5041 ccgccgtgcc ggcctttgca ggcgcgcaca acgtgctcga cgtgggcgca ggcggcggct
     5101 tgcccggcat cgtgctggcg atcgcgcgcc ccgagatgca gctcacgctg gtcgacaccg
     5161 tgcacaagaa gaccgccttc ctgacccagg tgaaggccga gctgggattg gcgaacgtga
     5221 gcgtgcacca tgcgcgcgtc gagcagatgc aggtcgcgca gaagtttgac gtgatcacct
     5281 ctcgcgcctt tgcggaactg aatgatttcg tcacctggtc cggccacctg ctcgccgacg
     5341 gcggatgctt catcgccctg aagggcgtgc ggcctgacga cgaagtcgcg cgcctgccgg
     5401 ccggctggaa gctcgcaaag ctcgtaccgc tcgacgtgcc cggactgggc gccga
//
LOCUS       DATLGF010000491         4514 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_218548, whole genome shotgun sequence.
ACCESSION   DATLGF010000491 DATLGF010000000
VERSION     DATLGF010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218548"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VM406_15255"
     CDS             <1..499
                     /locus_tag="VM406_15255"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL77370.1"
                     /translation="LWSELQVRRFLFDDQPVSMDVARATLNICLSRRETGTGLWVLRL
                     ADNGAPVGCAGLNPAAASTSHEPRLAGLLEPLAALHPEHWHRGYAIEALDALLRHAFE
                     TLGQERVGAANDLPNLASARMLERLGFVPIGKVQGPRYRMRTYILTREAWRRRMRRGA
                     SPDEG"
     gene            590..2491
                     /locus_tag="VM406_15260"
     CDS             590..2491
                     /locus_tag="VM406_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77371.1"
                     /translation="MQGPHASHFQSAHHAHGASSGQPFHAQPQRPQLTAQQYVQALEQ
                     HFQIPGGSVDYSALHRHLMGQGPDAQKAMLDHSHAYRDFLGVANENGLRKLFQQNPGP
                     RITYPEAPQALAGAATLAGKQSTANRALNQLQILLWQVPPHADASAHGHAYRQSVETA
                     LVNLLGNAERLTPENRLKAARIVNQHIRSLDPAAQARVGRAAADTAQRLGGHQPELRA
                     LLEPLAPPSPLRNAGRNHFFRQTGSAAPQTPPPHAGANPARLLGSLKQAGWTTAHLDA
                     LRNTLMQHQQAIDHGVDPRNVVARFNQEYPTLAPLFPVADPRSAQAMSHMLGALKMEH
                     NATISPQQWHAELKQAGWSAQSGGHLDQLRATILGCLHAARAGAPPAQALQAFAARYP
                     GMIDPAHPQALAQLHGLAQAIERDLHAHAAPAPASNPDAAIYTKSSSELVDAMKARFS
                     PQQLAQMSQALGQLWASDTPEGREAVKLYNAQLKPILGENIEPLLAPLSSQERQDPAA
                     LRRREKQEEAFGNLRSELNEHLNKLRQAKPAQAEASNAAARRFPHYGGSPIAIERGAD
                     MQQVLQQLAQHLSTEQLRELREAIRDCASRGTLSADDFDRQYGNLFSTSIAGDAKAGY
                     ARLMRVLPK"
     gene            complement(2564..4156)
                     /locus_tag="VM406_15265"
     CDS             complement(2564..4156)
                     /locus_tag="VM406_15265"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HVL77372.1"
                     /translation="MNLRNLRISRRLGLAFGAILAILLLTVGASVYLLSENKARLIQG
                     LETANAKAVVVADMKSALLEGGIAMRNMLDITSFDRHKSRADQQNARYAAARERVTKL
                     NLTEQERALLDAIVALDEQVASRYQMAVRQAENMNTEGAASLITNHIDPANLKAVEEM
                     DKLVRLAEAAARQVLDDSVRADHQLMMLMALISVIAVAIGAALAWLITRSITQPLDEA
                     VAIAESVARGDLTSEVSDDAADEIGRLLGALRQMNMNLREIVSKVRNGTDAISAASSN
                     IAEGSADLSARTEQQASSLEETAAAMEELTSTVNNNAESARTATQLASSASEVAEKGG
                     VAVNNVVRTMEGIKDSARKIADIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVV
                     ASEVRNLAQRSAVAAKEIKALIDDSVGKVEAGSKLVNDAGATMDEVVQGVERVTRIIT
                     EIMEATQEQSQGIGQVNQAMSQMDQVTQHNASLVEETATACHSMQEQAVALAQIVSVF
                     RVSHTAEDKQPSASRELVLASH"
BASE COUNT          764 a         1544 c         1478 g          728 t
ORIGIN      
        1 gctctggtcg gagctgcagg tgcggcgttt tctgttcgac gatcagccgg tgtcgatgga
       61 tgtcgctcgg gcgacgctca atatctgcct gtctcgccgc gagacgggaa cgggtctgtg
      121 ggtgctgcgc ctggccgaca acggcgcgcc ggtcggctgc gccggtttga atccggcggc
      181 ggccagcacc agccatgaac cgcgcctggc cggactgctg gagccgctgg ccgcccttca
      241 ccccgagcac tggcatcgcg gctacgcgat cgaagcgctc gacgcgctgc tgcgtcacgc
      301 gttcgaaacc ctcgggcagg aacgcgtggg cgccgccaac gatcttccaa acctcgcgtc
      361 ggcgcgaatg ctggagcgtc tcggctttgt gccgatcggg aaggtacagg gtccgaggta
      421 ccggatgaga acgtacatct tgaccaggga agcctggcgc aggcgcatgc gccgcggcgc
      481 gagccccgac gagggctgag cacgtccggg gatgcgggaa ccgacgaagc ggacgcggcc
      541 actcatcggt caactgcaac gctcctctgc gcattcaggg aaaccgacca tgcaagggcc
      601 gcacgcctcc cacttccagt ccgctcacca cgcacatggc gcaagcagcg ggcaaccgtt
      661 ccacgcgcag ccgcagcgtc ctcagctcac cgcgcagcaa tacgtgcaag cgctcgaaca
      721 gcacttccag atccccggcg gcagcgtcga ctattccgcg ctgcaccgcc atctgatggg
      781 acagggaccc gacgcgcaga aagccatgct ggaccacagc cacgcatacc gcgactttct
      841 cggcgtggcg aacgagaacg gactgcgcaa gctgttccag cagaatcccg gaccgcgcat
      901 cacctatccg gaagcgcccc aggccctggc aggagccgcg accctcgcgg gcaagcaaag
      961 caccgcgaac cgcgcgctca accaactgca gattctgctg tggcaagtgc cgccgcatgc
     1021 ggacgcaagc gcgcacggcc acgcctacag gcagtcggtg gaaacggcct tggtcaacct
     1081 gctgggcaat gcggaacggc ttacgcccga gaatcggctc aaggccgcgc gcatcgtcaa
     1141 tcagcacatc cggtctctgg acccggccgc ccaggcccgg gtcggccgtg cggccgcaga
     1201 caccgcgcag cggctcggcg gccaccagcc ggagctgcgc gcgttgctgg aaccgctggc
     1261 accgccgtcg ccactgcgca atgcgggcag gaaccatttt ttcagacaga cgggcagcgc
     1321 cgcgccacag actccccctc cccatgccgg cgcgaacccg gcccggctgc tcggctcgct
     1381 caagcaggcc ggctggacga cggcgcacct ggatgccttg cgcaacacgc tgatgcagca
     1441 tcagcaggca atcgaccacg gcgtcgatcc gcgcaacgtc gtcgcacgct tcaatcagga
     1501 atatccgacc ctcgcgccct tgttcccggt cgccgatcca cgctcggcgc aggccatgtc
     1561 gcacatgctg ggcgcgctga agatggaaca caatgccacg atatcgccgc agcaatggca
     1621 tgccgagctc aagcaggccg gctggagcgc gcagtcgggc gggcacctgg atcagctgcg
     1681 cgcaacgatc ctcggctgcc tacacgccgc ccgtgccggc gcaccgccgg cacaggcgtt
     1741 gcaggctttc gcggcgcgct atccgggcat gatcgaccct gcccatccgc aggcgctggc
     1801 gcagctgcat ggactcgcac aggcgataga acgggatctg catgcgcacg ccgcgcccgc
     1861 gcccgcttcc aatccggacg ctgcgatcta cacgaaatcg tcgagcgaac tggtcgatgc
     1921 aatgaaggcg cggttcagcc cgcaacagct ggctcagatg tcgcaagcgc tcggccagct
     1981 gtgggccagc gacacgccgg agggacgcga ggcggtcaag ctgtacaacg cgcagctcaa
     2041 gcccatcctt ggcgagaaca tcgaaccgct gctcgccccg ctctcgtcgc aggaacggca
     2101 ggatccggcc gcgctgcgcc gccgcgaaaa gcaggaggaa gcattcggca acctgcgcag
     2161 cgaactcaat gaacacctga acaagctgag gcaggcaaag cccgcgcagg ccgaggcaag
     2221 caacgccgct gcgcgcagat tcccgcatta cggcggcagc ccgatcgcga tcgagcgcgg
     2281 cgccgacatg cagcaggtgc tgcagcagct ggcgcagcac ctgtcgacgg agcagttgcg
     2341 ggaattgcgt gaagcgatcc gcgactgtgc aagccggggg acgctgtcag cggacgattt
     2401 cgaccggcag tatggcaact tgttctcgac aagtatcgcc ggcgacgcga aggcgggata
     2461 cgcgcggctg atgcgcgtgc tgccgaaata aggccggtca gctaccagcc cggcagcgac
     2521 gcagcgtgcc ggccgcatgc gggcgccggc acgcgcgggg ggatcagtga gacgccagca
     2581 ccagttcgcg cgaggccgac ggctgcttgt cttcggccgt gtgcgacacg cggaacacgc
     2641 tgacgatctg ggcgagcgcc accgcctgtt cctgcatcga atggcaggcc gtcgcggttt
     2701 cctcgaccag cgaggcattg tgctgggtga cctgatccat ctggctcatc gcctgattga
     2761 cctggccgat gccctggctc tgctcctgcg tcgcttccat gatctcggtg atgatgcgcg
     2821 tcacgcgctc gacgccctgc acgacctcgt ccatcgtggc gccggcgtcg ttgacgagct
     2881 tgctgcccgc ttcgaccttg ccgacagagt cgtcgatcag ggccttgatc tccttggcgg
     2941 ccactgccga gcgctgcgcg aggttgcgca cttcgctggc gacgaccgcg aagccgcggc
     3001 cctgctcgcc ggcgcgcgcg gcttccaccg cggcgttgag agcaagaata ttcgtctgga
     3061 atgcgatgcc gtcgatgacg ccgatgatgt cggcgatctt gcgcgccgag tccttgatgc
     3121 cttccatcgt gcgcacgacg ttgtttaccg ccacgccgcc cttttcagcg acttccgagg
     3181 cggacgacgc cagctgggtg gcggtgcggg cgctttcggc attgttgttc acggtcgagg
     3241 tcagttcctc catcgccgcc gcggtttcct ccagcgagct ggcttgctgc tcggtacggg
     3301 ccgacaggtc ggcgctgcct tccgcgatgt tcgaggacgc ggcgctgatc gcgtcggtgc
     3361 cgttgcgcac ctttgaaacg atctcgcgca ggttcatgtt catctggcgc agcgcgccca
     3421 gcagacggcc gatctcgtcg gccgcgtcgt cgctgacttc cgaggtcagg tcgccgcgcg
     3481 cgacgctttc cgcgattgcc accgcctcgt cgagcggctg cgtgatgctg cgggtgatca
     3541 gccaggccag cgcggcgccg atggccaccg cgatcacgct gatcagcgcc atcagcatca
     3601 tcagctggtg gtcggcacgc accgaatcgt ccagcacctg ccgcgcggcc gcctcggcca
     3661 ggcgcaccag cttgtccatc tcttcgaccg ccttcaagtt ggccggatcg atatggttgg
     3721 tgatcagcga tgccgcacct tcggtgttca tgttctcggc ctggcgcacc gccatctggt
     3781 agcggctcgc gacctgctcg tcgagcgcga cgatggcgtc gagcagcgcg cgttcctgct
     3841 cggtcaggtt cagcttagtc acccgctcgc gtgccgccgc atagcgcgca ttctgctggt
     3901 ccgcccgact cttgtggcgg tcgaaggagg tgatgtcgag catgttgcgc attgcgatcc
     3961 cgccttcaag cagcgcgctc ttcatgtcgg cgacaaccac ggccttggcg ttggccgtct
     4021 ccaggccttg aatcaggcgt gccttgtttt cgctgagcag gtagacagag gcgcccaccg
     4081 ttagcaggag gatggccagg atggcgccga aggccaggcc gaggcggcgg gagatgcgta
     4141 aattgcgcag attcatactt tttccactat tgcattgtca gactgtccag cgggtgttcg
     4201 cacgtctctg gacaaggcgc gagttcggca gtatatgatg gcggcgacga tattgccata
     4261 cggcaatagt ggtccgaaaa cgacacaacg gtgcaacacc gtcgcgcgtt ccggcaccag
     4321 gcagcggctg gcgcccgccc ggtactgcgg catgccggca gcagcgtggc cggccgcgct
     4381 attcgggacg cgcgcgcagc gccgcctcat aggcggggct gaatgaaatc gtgttgtgcg
     4441 cggtgcccgg gatcacgagc agccgcgcga cgcccggcgc aaagcgcccg gccagccgct
     4501 gcctggtgcc ggaa
//
LOCUS       DATLGF010000492         6820 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_123279, whole genome shotgun sequence.
ACCESSION   DATLGF010000492 DATLGF010000000
VERSION     DATLGF010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6820
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123279"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VM406_15270"
     CDS             <1..236
                     /locus_tag="VM406_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HVL77373.1"
                     /translation="SYGCGMGKCTKCACIVLAGGENLPAPNWKEKKRLGERLAQGYRL
                     ACQLWITHDVELTQDGIDPGLAERAQLEGVRDA"
     gene            229..582
                     /locus_tag="VM406_15275"
     CDS             229..582
                     /locus_tag="VM406_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HVL77374.1"
                     /translation="MRDGPSPASAVLLTDAAVDAVEITFLTNDGKVAKAPANSNLLRV
                     SLREKGGIPFKCGGGKCGTCKCLIEDGLENLAPVKDKERDHLSESELARGYRMACQTT
                     ILQGSVKVSWKGPVR"
     gene            652..1395
                     /locus_tag="VM406_15280"
     CDS             652..1395
                     /locus_tag="VM406_15280"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVL77375.1"
                     /translation="MHYPMTSLALIAITFILAGMVKGMIGMGLPTVGMALLAPVMGPA
                     ESVALLVLPTLVTNVWQLVAGPRFLYLLRRFGTLVAGLFVGSFFGVRLLADPDLASIL
                     LGAVLAAYGVLGLSRARFSVPDRWERRLSPVTGIVTGVLYGSTGLAVTAVPYVNSLRL
                     DKDALIQTLGLTFAVCSFGMALGLVAAGRFQTTVAGTSVLALLPAFAGMMIGQRLRER
                     MNADTFRRYFFLSICALGIYMVWHTLAKG"
     gene            1582..2430
                     /locus_tag="VM406_15285"
     CDS             1582..2430
                     /locus_tag="VM406_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HVL77376.1"
                     /translation="MNQRQHRLATRFRDLHVGGTSFVIPNPWDIGSARMMCHLGFPAL
                     AGSSAGCAFSNGVEDGKVARERMLGHIAEIAGACELPFSADLGNGFGDDPETVAETIR
                     LAAAAGAVGASIEDATGRRDDPVYPLDVAVERIEAAVAAARRLPFPFVLTARADNFII
                     GREDLDDTIRRLQAFQQAGADVLFAPGLRRLEDIRAVVAAIDRPLNVMMGLPGIEYAV
                     ADLRAAGATRVSLGASLARRAFAALHDALTEVSGPGTFTYAGQALAAGDLVRMFRAGH
                     VDPAQA"
     gene            complement(2792..3616)
                     /locus_tag="VM406_15290"
     CDS             complement(2792..3616)
                     /locus_tag="VM406_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77377.1"
                     /translation="MLARTLDKFARNINGDVFTRRKSVTITEFPQGVEVLVNENHETC
                     SDGEMYVEVQRAERGEGDREKNIARAARRAKTQVRRRCKMIGADCMLTLTYRENMQDV
                     ERLERDVKAFVKRLRDLGPFEYVLCIEPQKRGALHVHMACQSFPAWMVNSDGVRVKSF
                     NLIRSIWRRVVGRDNGNVDVTRPRGRNAAHRIASYIAKYVAKNVEDSELNAKRYWSSK
                     GIPAPKVTRIWFDGSMPTVDMLELVMADFLGRGYSDFTHYQDRFNEFHWFAASKPS"
     gene            complement(3930..4775)
                     /locus_tag="VM406_15295"
     CDS             complement(3930..4775)
                     /locus_tag="VM406_15295"
                     /inference="COORDINATES: protein motif:HMM:NF017518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HVL77378.1"
                     /translation="MSDYIVTGKKGNGKSLVCVGKIRDALMAGRAVATNLDLKLDKLL
                     PPHIKNVRCYRLPDYPSVEDMEALGMGSDKLDESSYGLIVVDECATFLNTREWGDKKR
                     QPLLNWFVHSRKYRWETMFIAQNINQLDKQLREALADHHVVCKRIDKLRIPFLGPLTK
                     HVLGFELRPPKVHVAAVRYGMEHSALITDRWTYRGVGLYAAYDTEQVFDPEYPHGLYS
                     YLSPWHIAGRHGEREFRLRDLFKPAKPRRRELKPKRPIVALLQQLPPDERIKHLKRFQ
                     AAGVV"
     gene            complement(4778..5104)
                     /locus_tag="VM406_15300"
     CDS             complement(4778..5104)
                     /locus_tag="VM406_15300"
                     /inference="COORDINATES: protein motif:HMM:NF037579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5455 family protein"
                     /protein_id="HVL77379.1"
                     /translation="MLKLISMLATGIPAVIGGLIAFLGRKAGVAAASIASFVIITMGL
                     ILCLNAILQALLAMLVIPAWLANSIGLFIPINWSACLAAIVSSKICRAAYEMAKFKIV
                     AINNAS"
     gene            complement(5104..6621)
                     /locus_tag="VM406_15305"
     CDS             complement(5104..6621)
                     /locus_tag="VM406_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77380.1"
                     /translation="MRSAFKLLPVAFVAALLLPLPALADYAGLKQINDIIRGAPGVST
                     VATGAGVSATASGVHTASTNGLKIPVPTSATAHIPKASVATAARAAVRLMGPIGTAAT
                     AYNIYRELQDSGLHTCPPPDFFCKQVPEQVNPRPAHHRLYFEVTPGKNYAQEIDACWD
                     SKTVQYDNNPNGDARVKTATSVKVGAETTYQGNVVKPCQTVGGTQPNYVYNYVRFHQW
                     LSCPSGYTLNTATNMCVSNTTVTRPFSDPEFDQELLDRINANPERAKRYYDAIQNDLD
                     RTWYPLPREALVPTNTPVDVSAPPVSTPQRTVQTETITKPDGTTETRTKTEQTTVTPQ
                     RTGTTTGNTQVTYPSSTTQITTIVNNTTNETTTHTTVINHPAESPSELEIPEDYAREG
                     TLGEVLEAIRADGAPDMEDQRPLVDSAVAKHQGDLDKLYGDLPSGMAEDKDRFFSWVW
                     SPEIGVCSPFGGVVAGHEIVWDLCPTIEMIRDALGWLFALFSAWTVYGQMFKRYD"
BASE COUNT         1331 a         2058 c         2046 g         1385 t
ORIGIN      
        1 tttcctatgg ctgcggcatg ggcaagtgca cgaaatgcgc atgcatcgtg ctcgccggcg
       61 gcgaaaacct gccggcgccg aactggaagg aaaagaagcg gctcggcgag cggctcgcgc
      121 aaggctaccg gctcgcctgc cagctctgga tcacgcatga cgtggagctg acccaggacg
      181 ggatcgatcc cgggcttgcc gagcgcgcac agctggaagg ggtgcgcgat gcgtgacgga
      241 ccaagccctg caagcgcggt gttgctgacc gatgccgccg tcgacgcggt cgaaatcacc
      301 ttcctgacca atgacggcaa ggtcgcgaag gcgcctgcga acagcaacct gctgcgtgtg
      361 tccttgcgcg agaagggcgg cattccgttc aagtgcggcg gcggcaagtg cggcacctgc
      421 aaatgtctga tcgaggatgg gctggagaat cttgcgccgg tgaaggacaa ggagcgcgat
      481 cacctgagtg aaagcgagct cgcgcgcgga taccggatgg cctgccagac cacgatcctg
      541 caaggcagtg tcaaggtgtc atggaagggc ccggtcagat gatgaaacgg cagccagagg
      601 ccttccgttt ccgtccgcac gactgccggt gaccgctgca ggatggcggc catgcactat
      661 ccgatgacga gtctggcgct gatcgccatc accttcattc tcgcgggcat ggtgaagggc
      721 atgatcggca tgggcctgcc gacagtgggg atggccttgc tggccccggt gatggggccg
      781 gccgaatccg tcgcgctgct ggtgctgccg acgctggtga ccaacgtctg gcagcttgtg
      841 gccggaccgc gcttcctcta cctgttgcgc cgcttcggca ccctggtggc cggcctgttc
      901 gtcggatcat tcttcggcgt acgcctgctt gccgatccgg atcttgcatc gatactgctc
      961 ggcgcggtgc tcgcggccta tggcgtgctc gggctgtcgc gtgcccgatt cagcgtgccg
     1021 gatcgctggg agcgacgctt atcgccggtg accggcatcg taaccggcgt gctgtacggc
     1081 tcgaccggac tggcggtaac cgcggtgccg tacgtgaatt cgctgcggct ggacaaggat
     1141 gcgctgatcc agacgctcgg cctgaccttc gcggtctgct cgttcggcat ggcgctcggt
     1201 ctcgtggcgg ccggacgctt ccagacaaca gtcgccggca cctcggtgct ggcgctgctg
     1261 ccggccttcg ccggcatgat gatcggccaa cgcctgcgtg agcggatgaa tgccgacacc
     1321 ttccggcgct atttcttcct gtcgatctgc gcgctcggca tctacatggt ctggcacacg
     1381 ctggccaagg gctgagagcc gcgcaggcgg gcgccggcgc cgctgctgga agctggcgtt
     1441 tcaatcccgc tcgccatcct cgaagttcgt gaacgcttga ggcagaccat gcgcgccgcg
     1501 cattgaagtg gcgcggagaa atcatttgag cagcggcccc gcggcttcgt agacttgcaa
     1561 gatcaccggg tggaggaagc catgaaccag cgccagcatc gccttgccac gcgttttcgt
     1621 gatctgcacg tgggcggcac cagcttcgtg atcccgaatc cttgggacat cggaagcgcg
     1681 cgcatgatgt gccatctcgg ctttcctgca cttgccggga gcagcgccgg ctgcgcgttc
     1741 tcgaacggcg tggaagacgg aaaggtggcg cgcgagcgca tgctcggcca tatcgcggag
     1801 atcgccgggg cgtgcgaatt accgttcagc gccgatctcg gcaacggctt cggcgacgac
     1861 ccggagacgg tggccgaaac tatccgtctg gcggccgccg ccggcgcggt cggcgcttcc
     1921 atagaagacg ccaccggccg ccgcgacgat ccggtgtatc cgctggacgt cgctgtggag
     1981 cggatcgagg cggctgtcgc ggcggcgcgc cggctgccat ttccattcgt gctcaccgca
     2041 cgcgccgaca acttcatcat cggccgagag gatctggacg acaccatacg gcggctgcag
     2101 gcgtttcagc aggcaggggc cgacgtgctg tttgccccag gactgcgccg gctggaggac
     2161 atccgcgctg tggtggccgc gatcgaccga ccgctgaacg tcatgatggg gcttcccggc
     2221 atcgaatacg cggttgccga cctgcgtgcg gcaggtgcga cgcgcgtcag cctcggtgct
     2281 tctttggcgc gtcgcgcgtt cgccgcattg catgacgccc tgaccgaagt ttccgggcca
     2341 ggcacgttca cctatgcagg gcaggcattg gccgccggag atctggtgcg catgttccgt
     2401 gcgggacacg tggacccggc gcaggcgtag tggcctgttg ggtggtcaca cacggagccg
     2461 tataatttgt attatgttaa atcggatgcg caagtttgcc cgcgccgtca ttgacgcatt
     2521 gaaggcagcg ctgagcccga caaccggacc tgcagcacgc tagaaaatcc aatacttccc
     2581 gcttgctaaa gcctgccatg agcaggcttt ttttatgggc cgcgacgatc cgataatgcc
     2641 gacgctgctg agccccacca taccgcgctt ttctcaggtt cctcaggaac caatactcgc
     2701 gccgtatccc tgctagaagt gcctcccggg cctctggaga caatggttca tacccgaata
     2761 agtctaactg catcgggccg ggattatgcc atcatgaagg cttcgacgca gcgaaccaat
     2821 ggaactcgtt aaaccggtcc tgatagtgcg tgaaatctga atagccgcgc ccaagaaagt
     2881 ccgccatgac gagttcgagc atatccacgg taggcatgct gccgtcgaac cagatacgcg
     2941 tgaccttagg cgcaggtatg cccttgcttg accaataccg cttggcattg agttcggaat
     3001 cttcgacatt ctttgcaacg tactttgcga tgtaggaagc gatccgatgc gccgcattgc
     3061 ggccgcgtgg cctagtcacg tccacattgc cattgtcacg accgactaca cgccgccaga
     3121 tcgagcgaat caggttgaag ctcttgaccc gcacgccgtc gctattaacc atccatgccg
     3181 ggaacgattg gcacgccata tgcacatgca gagccccgcg cttttgaggt tcgatgcaaa
     3241 ggacgtactc gaacggcccg aggtcgcgaa gccgcttcac gaaggctttc acatcacgct
     3301 ctaggcgctc tacgtcctgc atgttctcgc gatacgtgag cgtcagcatg cagtctgccc
     3361 cgatcatctt gcagcgtcgg cgcacttgcg ttttggcccg ccgcgccgcc ctggcgatgt
     3421 ttttctcccg gtccccttcg ccgcgctccg cacgctgaac ctcgacatac atctcgccgt
     3481 ctgaacaggt ttcatggttc tcgttcacaa gcacctcgac cccttgagga aactcagtga
     3541 tcgtcacgga tttacgccgg gtgaagacat cgccattgat attgcgagcg aacttgtcta
     3601 aagtgcgggc aagcattcaa tctcctttag ctttgtggat gcgagccccg gcgacttggc
     3661 agagttgacc ggggcatttt ttttacttct ttcctgcttt tgttctttaa gtgtcctagg
     3721 tataaatcta gggcgcctgc ggcgcccgct agcgcagtcg cctccgccgc cctagaacaa
     3781 cgccctcccc gtaatgcggg agcattgata ggcgacacga tctgcgcaga ccaccatggg
     3841 cctgtccgcc cgcaggtcct tacccatcgg atgcggggcc gcacagccca ccagcagcgc
     3901 acagagcgca atcaccgacg cacgacgcat cacacaaccc ccgcagcctg aaaacgcttc
     3961 agatgcttta tccgttcgtc tggcggtaac tgctgcagca gagccacgat aggccgcttg
     4021 ggctttagtt cacgcctacg aggctttgca ggcttgaaca gatcccgcaa ccggaactcc
     4081 ctttcgccgt gccgccctgc gatatgccag ggtgagaggt acgagtacag cccatgcgga
     4141 tactcaggat caaagacttg ctcagtgtcg taagccgcat agagtccgac gccgcgatag
     4201 gtccaccggt cagtgatcag cgcggaatgc tccatgccat agcggaccgc cgcgacatgc
     4261 acttttggcg gacgcaactc gaacccgagc acatgctttg tcaacgggcc gaggaatggg
     4321 atccgtagct tgtcgatgcg cttgcataca acgtgatgat cggcgagcgc ttcccgtagc
     4381 tgcttatcga gctggttgat gttctgcgcg atgaacatgg tctcccaccg gtacttgcgg
     4441 gagtgaacga accagttcag cagcggttgc cgtttcttgt cgccccactc acgcgtattg
     4501 aggaaggttg cgcattcatc gacgacgatc agcccataag acgattcatc gagcttgtcc
     4561 gagcccatgc cgagcgcttc catatcctcg acgcttggat agtccggaag ccggtagcaa
     4621 cggacgttct tgatgtgcgg cggaagcagc ttgtctagct tgagatcaag gttcgttgca
     4681 accgccctac ctgccatcaa tgcgtcgcgg attttgccga cgcagacaag gcttttgccg
     4741 ttgccctttt tgcctgtcac gatgtagtca ctcatggtta gctcgcattg ttaattgcaa
     4801 cgatcttgaa cttagccatc tcgtaagccg cccggcagat cttgctagag acgatggcag
     4861 cgaggcatgc cgaccaatta attgggatga acagcccgat agagttggcc agccatgcag
     4921 gaataacgag catcgcaagc agcgcttgca ggatcgcgtt gaggcacagg attagcccca
     4981 tggtgatgat cacgaacgat gcgatcgaag cggcagcgac accagctttg cgaccgagga
     5041 aggcgatcaa cccaccgatt acagccggta tgcccgtcgc tagcatggaa ataagcttga
     5101 gcatcagtcg tacctcttga acatttggcc gtagaccgtc caggccgaaa acaacgcgaa
     5161 aagccagccc aacgcatcgc gaatcatctc aatcgttgga cacagatccc agacaatctc
     5221 gtgccccgcg accacaccgc cgaacggaga gcagacccca atttcaggag accaaaccca
     5281 tgagaaaaaa cggtccttat cctcagccat tccggatggc agatcgccat acagcttatc
     5341 tagatcgccc tggtgcttcg cgaccgcact atcgacgagc ggacgctgat cctccatatc
     5401 cggagcgccg tcggcacgaa tggcctcgag cacttcgcct aacgtgccct cccgtgcgta
     5461 atcctcagga atctccaatt cactaggcga ctcagccgga tgatttatga cagtagtgtg
     5521 cgtcgtggtc tcgttggtgg tgttattgac gatcgtggtg atctgagtcg tgctagacgg
     5581 atacgtgact tgagtgttac cagtcgtggt cccggtacgc tgaggcgtga cggtggtctg
     5641 ttcagtctta gtccttgtct cagtggtacc gtccggcttt gtaatggtct cagtttgcac
     5701 tgtcctttgt ggcgtgctta caggcggagc actcacgtcc acgggggtgt ttgtcggaac
     5761 aagcgcctca cgcggcagcg gataccaagt gcgatccaaa tcgttctgaa tagcatcgta
     5821 gtaacgcttc gccctctcag gattggcatt aatccgatcg aggagctctt ggtcgaattc
     5881 ggggtcagag aacggcctag taacagtcgt gttcgacacg cacatatttg tcgcggtatt
     5941 cagcgtgtac ccggacggac aactcagcca ctgatgaaac ctcacatagt tgtacacata
     6001 gtttggctga gtgcccccga ctgtctgaca cggcttcaca acgttaccct gataagtggt
     6061 ttcagcccct accttgacgc tagtcgcggt ctttacacgg gcgtcgccat tgggattgtt
     6121 atcgtactgg accgttttcg agtcccagca cgcgtcaatc tcctgagcgt agttcttacc
     6181 gggagtcacc tcaaagtaga ggcgatgatg ggcgggccga ggattcactt gttcgggaac
     6241 ctgcttacag aagaagtccg gaggcgggca agtgtgcaaa ccgctgtcct gcagctcgcg
     6301 atagatgttg taagccgtag ccgctgtacc aatgggcccc atgaggcgaa cagcagcacg
     6361 cgcagcggta gcaacagacg ccttaggaat atgtgccgtt gcggaggtag gaactgggat
     6421 cttcagcccg ttcgtgctcg cggtatgaac gccggaagcc gtagcagaga caccagcgcc
     6481 cgttgcgaca gtcgaaacac ccggagcgcc gcgaatgatg tcattgattt gcttgagacc
     6541 cgcgtaatcc gcaagcgcag ggagtggaag gagcaaagcc gccacaaagg cgaccggaag
     6601 cagtttgaag gcagagcgca ttgcgaatcg tcccgacagt gttagcgtcc gagttggtct
     6661 atagggcgac ggtggtggac gtggaccgtc gaaccttcgg gccagcgccg ccacgctgac
     6721 cgcctgaaaa tcccgcgggc cgccttgaag gcgacaacct ccacgcctcc ggacagtgtt
     6781 cccccttccc cgcacgcggg gcccccttag tggcccgaat
//
LOCUS       DATLGF010000493         5570 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_78732,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000493 DATLGF010000000
VERSION     DATLGF010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78732"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..781)
                     /gene="sodB"
                     /locus_tag="VM406_15310"
     CDS             complement(203..781)
                     /gene="sodB"
                     /locus_tag="VM406_15310"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HVL77381.1"
                     /translation="MEHTLPPLPYAKNALQPHISEETLEYHYGKHHQAYVTNLNNLIK
                     GTEFENASLEDIIRKSSGGVFNNSAQVWNHTFFWNCMKPNGGGAPGGALADAINAKWG
                     SFDKFKEEFSKSAVSNFGSGWTWLVKKGDGSVDIVNTSNAGTPLTTADKALLTCDVWE
                     HAYYIDYRNARPKFVEAFWNVVNWEFAEKNFA"
     gene            complement(905..2266)
                     /gene="xseA"
                     /locus_tag="VM406_15315"
     CDS             complement(905..2266)
                     /gene="xseA"
                     /locus_tag="VM406_15315"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HVL77382.1"
                     /translation="MNPGLEEENRDAAPPVLTVSALNQAVSRLLERSFPLAWVSGEVS
                     NFTRAASGHWYFTLKDNAAQVRAVMFRGRAQYAGFTPREGDKVEVRALVTLYAPRGDY
                     QLNVEAIRRAGIGNLYEAFLRLKAKLEAEGLFDPARKRPLALFARTIGIVTSPQAAAL
                     RDILTTLRRRAPHLRVILYPAPVQGAGAAEKIAEAIGTASARAECDVLLVCRGGGSIE
                     DLWSFNEEVVARAIVACSMPVIAGVGHETDFTIADFAADLRAPTPTAAAEMAAAPREQ
                     WLQMLQAHADDLGRALVRRHGDAGQRLDWLARRLVSPAAYLQRERLRLRGMQAGLGHA
                     LHAPVQRARLALARLHARLDRQLPDTAVGRSRLTHAQQRIQQQLAAALLQHRQRLASL
                     QAQLSLLDPQRTLERGYAIVQDDKGQVVRSPRQLRPNSTISVRVAEGSAELGIASVQP
                     RLE"
     gene            2580..3185
                     /locus_tag="VM406_15320"
     CDS             2580..3185
                     /locus_tag="VM406_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HVL77383.1"
                     /translation="MLAIIQAAGWPIYFLIITSIIALALIIERVLYLRRARILPPNLL
                     DEVVRVHRGGKVGKEVLDNLEQNSPLGRVLAAGLRNVNAPREVMKESIEEAGRGTAHE
                     LERFLTSLGTIATLAPLLGLFGTIVGMIEIFGSQSAVGTNPGQLAHGISVALYNTGFG
                     LAIAMPALVFYRHFRAQVDSFVLDMEQQAVKFVDIVHGNRR"
     gene            3195..3629
                     /locus_tag="VM406_15325"
     CDS             3195..3629
                     /locus_tag="VM406_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HVL77384.1"
                     /translation="MNFRKGRVREAPEINLIAFIDVLLVILIFLMVTTTYSKFTALQI
                     TLPTADAEKALEQPLEINVAVDARGQYAVDNTQIAARDAMGLAAELANTAAARGQDRR
                     DPVVIINADASAAHQAVINVLEAARLAGFEKVTFAAQASGKR"
     gene            3661..4695
                     /gene="lpxK"
                     /locus_tag="VM406_15330"
     CDS             3661..4695
                     /gene="lpxK"
                     /locus_tag="VM406_15330"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HVL77385.1"
                     /translation="MPAPHSLLETTLTRAWLARGALAWLLVPVALLFLCIVTLRRALY
                     RAGLLRSERVGVPVVVVGNVFVGGTGKTPFTIWLVQALRGLGFVPGVVSRGYGASSRK
                     PTLVNPSSSAAEVGDEPLLIARRAACPVVVGRERVAAARALLAAFPNVNLIIADDGLQ
                     HYALARDVEIVLCDARGNGNGWLLPAGPLREPASRRRDFTVVNGDVIPPGLPQPVFQM
                     RLNAPQALHLADAREQRPLPAFAAAGLRVAAAAGIGNPERFFTMLRDAGVPLADTLAL
                     PDHYDFAHDPFAHMHADAILITEKDAVKCLQLESFQRDPRLWVVPVSAEVDPALAHRI
                     VEKCRGLPTA"
     gene            4676..4867
                     /locus_tag="VM406_15335"
     CDS             4676..4867
                     /locus_tag="VM406_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HVL77386.1"
                     /translation="MDSRLLDILVCPICKGPLQYDKAAQELICNADKLAYPIRDGIPI
                     MWADQARDLNAPPAATNAA"
     gene            4896..>5570
                     /gene="kdsB"
                     /locus_tag="VM406_15340"
     CDS             4896..>5570
                     /gene="kdsB"
                     /locus_tag="VM406_15340"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HVL77387.1"
                     /translation="MSFTVIIPARLASTRLPNKPLADLGGKPMVVRVAERAALSGADR
                     VVVATDHADILAACREHGIQACMTRADHPSGTDRIAEVADALALPGDAVIVNVQGDEP
                     LIDPSLVAATAALIRTDVPMATAAHPIESASDAFNPNVVKVVLDRTGRALYFSRATIP
                     WHRDGFARATDVLPPDYTPLRHIGLYAYSRDFLRTYPTLQVSPLEQIEALEQLRVLWH
                     GYPIAVH"
BASE COUNT          881 a         1870 c         1822 g          997 t
ORIGIN      
        1 gtctatgtcg agcaggtcgc aaccagcgtc aacagcgtgg aggtgatcgg cagcgacgac
       61 gtgcggcaca tcattcggct gcgcgagccg ctgatgctgc cttcctgaac gtcgcggcgg
      121 cggcgcttga gacgaaaaaa agcccgcggt gcgtgtccgc gggcttgcag cggtatggcc
      181 gttatcgcgg ccacgaccgg gcttacgcga agttcttctc ggcgaattcc cagttcacaa
      241 cgttccagaa cgcttcgacg aacttcggac gggcgttgcg gtagtcgatg tagtacgcgt
      301 gttcccacac gtcgcaggtc agcagcgcct tgtcggcggt ggtcagcggg gtgccggcgt
      361 tggaggtgtt gacgatgtcg accgaaccgt cgcccttctt caccagccag gtccagccgg
      421 agccgaagtt gctgactgcg gacttgctga actcttcctt gaacttgtcg aacgagcccc
      481 acttggcgtt gatcgcgtca gccagcgcgc cgcccggtgc gccgccgccg ttgggcttca
      541 tgcagttcca gaagaaggtg tggttccaca cctgggccga gttgttgaag acgccgccgg
      601 aggatttcct gatgatgtct tccagcgacg cgttttcgaa ttcggtgccc ttgatcaggt
      661 tgttcaggtt cgtgacatag gcctgatgat gcttgccgta gtggtactcg agggtctctt
      721 cggagatgtg cggctgcagc gcgttcttgg cgtagggcag gggtgggaga gtgtgttcca
      781 tcgtcggtcc tttctctagt gcggttgatg ttcttttgca aagccaccaa ttgtacgtcg
      841 gatgccccgt cgctgcaggc cttcgggctt gggaaaagcc cgggccattg tgggacgtaa
      901 atgcttattc gaggcgcggc tgcacgctgg cgatgccgag ctcggcgctg ccctcggcca
      961 cgcgcacgga gatcgtgctg ttgggccgca gctggcgcgg cgagcgcacc acctggccct
     1021 tgtcgtcctg cacgatcgca tagccgcgct cgagggtgcg ctgcggatcg agcagggaaa
     1081 gctgggcttg cagcgacgcc agccgctggc ggtgctgcag caacgccgcc gcaagctgtt
     1141 gctggatgcg ctgctgcgcg tgcgtgagcc ggctgcggcc gacggccgta tccggaagct
     1201 ggcgatccag gcgcgcgtgc agccgcgcca gcgcgagtcg ggcacgctgc accggggcgt
     1261 gcagcgcatg gcccaggccg gcctgcatcc cgcgcaggcg cagccgttcg cgctgcaggt
     1321 aggcggccgg gctgaccagc cggcgcgcca gccagtcgag ccgctggccg gcgtcgccat
     1381 ggcggcggac cagcgcgcgg cccaggtcgt cggcgtgcgc ctgcagcatc tgcagccatt
     1441 gctcgcgcgg cgcggcggcc atttcggcgg cggcggtcgg cgtcggtgcg cgcagatcgg
     1501 ccgcgaaatc ggcgatcgtg aaatcggttt catggccgac gccggcaatg actggcatcg
     1561 agcaggcgac gatcgcgcgc gccaccactt cctcgttgaa ggaccacagg tcctcgatgc
     1621 tgcccccgcc gcgacatacc agcagcacat cgcattcggc gcgcgcggac gcggtgccga
     1681 tcgcctcggc gatcttctcg gctgcgccgg cgccctggac cggggccgga tagaggatca
     1741 cccgcagatg cggcgcgcgc cggcgcaggg tggtgaggat gtcgcgcagc gccgctgcct
     1801 gcggactggt gacgatgccg atcgtgcgcg cgaacagtgc cagcgggcgc ttgcgcgccg
     1861 ggtcgaacag gccttcggct tcgagtttcg ccttgaggcg caggaaggct tcatacagat
     1921 tgccgatacc ggcgcggcgg atcgcttcca cgttcagctg gtaatcgccg cgcggcgcat
     1981 acagcgtgac cagcgcgcgc acctcgacct tgtcgccttc acgcggagtg aacccggcat
     2041 actgggcccg gccgcggaac atcacggcgc gaacctgggc cgcgttgtcc ttgagggtga
     2101 agtaccagtg gccagaggcg gcgcgcgtga agttcgatac ttcgccggaa acccaggcca
     2161 gcggaaagct gcgctccagc aggcgggaga ccgcctgatt cagcgccgag accgtcagta
     2221 ccgggggcgc cgcatcgcgg ttttcttcct cgagcccagg attcatgcgg tctcccaagg
     2281 gaaaacatgt ccacaggcgc aacaacacgt cacttttctg taaaaattca tttccgcgcc
     2341 acgaatgttg gtgcaagtca ttgatctaaa agggaaattg cgctgagccc aatttccagc
     2401 cggcacgggt tttcccttta aaatcaaggc cttcccggcg ccatcgggca attgcccaca
     2461 aagttatcca cagtgctgtg aagaactttt ccaggagtaa cgctgtctgg agtgcttgct
     2521 cgcctccggg cgaaacgcta cattctgcat ccgtatcatc caccccgagg agctgtgttt
     2581 tgctcgccat catccaggcc gccggctggc cgatttactt cctgatcatc acttccatca
     2641 tcgccctggc cctgatcatc gagcgcgtat tgtacttgcg gcgcgcgcgc atcctgccgc
     2701 cgaatctgct cgacgaggtg gtgcgcgtcc atcgcggcgg caaggtcggc aaggaagtgc
     2761 tggacaacct ggagcagaat tccccgctcg gccgcgtgct ggccgccgga ctgcgcaacg
     2821 tgaacgcccc gcgcgaggtg atgaaggaat cgatcgagga ggccgggcgc ggcacggccc
     2881 atgagctcga gcgcttcctg acctcgctcg gcacgatcgc cacgctggcg ccgctgctcg
     2941 gcctgttcgg caccatcgtc ggcatgatcg agatcttcgg ctcgcagagc gcggtcggca
     3001 ccaatcccgg ccagctcgcc cacggcatct cggtggccct ctacaacacc ggcttcggac
     3061 tggcaatcgc gatgccggcg ctggtgttct accgccactt ccgggcccag gtcgatagtt
     3121 tcgtgctcga catggaacag caggcggtga agttcgtcga catcgttcac ggcaaccgcc
     3181 gctaaggtca ggtcatgaac ttccgcaagg gcagggtgcg tgaagcgccc gagatcaacc
     3241 tgatcgcatt catcgacgtg ctgctggtga tcctgatctt cctgatggtc accaccacct
     3301 acagcaagtt caccgcgttg cagatcacgc tgcccacggc cgacgccgag aaggcgctgg
     3361 agcagccgct ggaaatcaat gtcgcggtgg atgcgcgcgg ccagtacgcg gtcgacaaca
     3421 cccagatcgc ggcgcgcgat gcgatgggcc tggccgccga gctggccaat accgccgccg
     3481 cccgcgggca ggatcgccgc gatccggtgg tcatcatcaa tgccgacgcc tcggccgcgc
     3541 accaggcggt gatcaacgtg ctcgaagcgg cgcgcctggc cggcttcgag aaggtcacct
     3601 tcgccgccca ggccagcggc aagcgctgaa cggccgcggc gcttccttcc cgaaacgacg
     3661 ttgcccgcgc cgcattccct gctcgaaacg acgctcaccc gcgcatggct tgcgcgcggc
     3721 gcgctggcct ggctgctggt gccggtcgcg ctgctgttcc tctgtatcgt cacgctgcgc
     3781 cgggcactct accgcgccgg cttgctcagg agcgaacgcg tcggcgttcc ggtcgtcgtg
     3841 gtagggaatg tgttcgtggg cggcaccggc aagacgccat tcacgatctg gctggtgcag
     3901 gcattgcgcg gcctcggctt cgttcccggc gtggtgtcgc gcggctacgg cgcgagcagc
     3961 cgcaagccga cgctcgtgaa tccctcttca agcgcggccg aagtcgggga cgagcccttg
     4021 ctgatcgcgc gccgcgccgc ctgcccggtc gtggtcgggc gcgagcgggt cgccgctgca
     4081 cgcgcgctgc tggcggcttt cccgaacgtc aacctcatca tcgccgacga cggcctgcag
     4141 cactatgcgt tggcacgcga tgtcgaaatc gtgctgtgcg acgcacgcgg caacggcaac
     4201 ggctggctgt tgcccgccgg gcccttgcgc gaaccggcgt cgcggcgccg cgatttcacc
     4261 gtcgtcaacg gcgacgtcat cccgcccggc ctgccgcaac cggtattcca gatgcgtctc
     4321 aatgcgccgc aggcgctgca tctggccgac gcgcgcgagc agcgcccgct gccggccttt
     4381 gccgctgccg gcctgcgtgt ggcggccgca gccggcatcg gcaaccccga gcgtttcttc
     4441 acgatgctgc gcgatgccgg cgtgccgctg gccgacacgc tcgccttgcc ggatcactac
     4501 gattttgccc acgatccatt tgcgcacatg cacgccgatg ccatcctgat cacggagaag
     4561 gatgcagtaa aatgcctgca actcgaatcc ttccaacgcg atccgcgcct gtgggtcgtc
     4621 cccgtgagcg ccgaggtcga ccccgcgctc gcgcaccgaa tagtggagaa atgtcgtgga
     4681 ctcccgactg cttgacatcc tggtctgccc gatttgcaag gggccgcttc agtacgacaa
     4741 ggccgcccag gaactgatct gcaatgccga caagcttgcc tatccgatcc gtgacggcat
     4801 tccgatcatg tgggccgacc aggcgcgcga cctgaacgca ccgcccgccg ccaccaacgc
     4861 tgcctgagcg cgccacccgc ttcgcaaact tcgcaatgtc tttcaccgtc atcattccgg
     4921 cacgcctggc ttccacgcgc ctgccgaaca agccgctggc ggatctgggc ggcaagccga
     4981 tggtcgtgcg cgtggccgag cgcgctgcgc tgtccggcgc cgaccgggtg gtggtggcga
     5041 ccgatcatgc cgacatcctt gccgcctgcc gcgagcacgg catccaggcg tgcatgacgc
     5101 gggccgacca tccgtccggc accgatcgca tcgccgaagt ggccgatgcg ctggcgctgc
     5161 ccggcgatgc ggtgatcgtc aatgtccagg gcgacgagcc gctgatcgat ccgtcgctgg
     5221 tcgcggcgac cgcggcgctg atccgtaccg acgtgccgat ggcgaccgcc gcccatccga
     5281 ttgaatccgc atccgacgcc ttcaatccga acgtcgtgaa ggtggtgctc gaccgcacgg
     5341 ggcgcgcact ctatttctcg cgcgcgacga ttccctggca ccgcgacggc tttgcccgcg
     5401 cgaccgatgt gctgccgccg gactacacgc cgcttcgcca catcggcctg tatgcgtaca
     5461 gccgggattt cctgcgcacc tatccgacgc tgcaggtttc cccgctcgaa cagatcgagg
     5521 cgctggaaca gttgcgcgtg ctgtggcacg gctatccgat tgcggtccat
//
LOCUS       DATLGF010000494         4139 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_218608, whole genome shotgun sequence.
ACCESSION   DATLGF010000494 DATLGF010000000
VERSION     DATLGF010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218608"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1972)
                     /locus_tag="VM406_15345"
     CDS             complement(<1..1972)
                     /locus_tag="VM406_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HVL77388.1"
                     /translation="MRAQGLYDPSNEHDACGVGFIAHIKGKKSHSIIEQGLLILKNLD
                     HRGAVGADKLMGDGAGILIQIPDQYYREEMAKQGVALPPPGEYGVGMVFLPKENASRI
                     ACEQEIERAVLAEGQVVLGWRDVPVDREMPMSPTVRAKEPVIRQIFIGRGPDIMVTDA
                     LERKLYVIRKSSGHAIQALNLIHGKEFFVPSMSARTVVYKGLLLADQVGVYYEDLQDP
                     RCVSALALVHQRFSTNTFPEWPLAHPYRYVAHNGEINTVKGNFNWIRAREGVMKSAVL
                     GEDLQKLFPLIYEGQSDTACFDNALELLVMAGYPVAQAMMMMIPEAWENHTLMDDNRR
                     AFYEYHAAMMEPWDGPAAMAFTDGRHIGGTLDRNGLRPARYIITDDDLVVMASESGVL
                     PIPESKIIKKWRLQPGKMFLIDLDAGRIIDDKELKDTYANAKPYKQWIDSVRIKLDEL
                     DAEPVEPKSELPLLDRQQVFGYTQEDIKFLMAPMAANGEEAVGSMGNDSPLAVMSNKN
                     KTLYNYFKQLFAQVTNPPIDPIREAMVMSLVSFIGPKPNLLDTNNINPPMRLEVAQPI
                     LDYADMAKLRNISKHTGGKFKSHELNICYPVAWGKEGIEARLASLCAKAVDAVRSGHN
                     ILIITDRKFDAEHVAIPALLATSAIHLHLVSKG"
     gene            complement(2128..2835)
                     /locus_tag="VM406_15350"
     CDS             complement(2128..2835)
                     /locus_tag="VM406_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVL77389.1"
                     /translation="MARLPRLVAPNQPHHVTQSGLDSLTIFRDAQDFQIFHGWLREAA
                     RQFKVSVHAYVVMPDHFQLLATPHDEHGLGRMMQWIGRKYVPYFNARYARQGTLWQGR
                     YKATVVDASSYLLLCSRYIEQLPVRAGLALSVADYPWSSASHHAGLKPDALITDHAAY
                     WALGNTPFAREAAYRALVDQNLSADEVGMLNGAVRRGWPLGPNSFQQDLARRLGRRVS
                     PSRRGRPARKAEAERKS"
     gene            complement(3015..3566)
                     /locus_tag="VM406_15355"
     CDS             complement(3015..3566)
                     /locus_tag="VM406_15355"
                     /inference="COORDINATES: protein motif:HMM:NF022337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HVL77390.1"
                     /translation="MAELGVFLVILGSGLLGWFILNRPRPARFRARPILTGSALEFLF
                     RLKRALPECTVCPQVAATSLLEPLGVGRARKAALSRIVGRRVGYAVFDEEMQLIAVIE
                     LDHRPRLTRREAECDSYFSEAGVPVLRFRQQQMPSVSKIRSSVFPRAAATVQCAEQSM
                     PDAAPIEFKPRAAIWRNTLNAHS"
     gene            complement(3665..>4139)
                     /locus_tag="VM406_15360"
     CDS             complement(3665..>4139)
                     /locus_tag="VM406_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVL77391.1"
                     /translation="LLTMGLETVYQGLRFSRCLVFLRRPERLRYVAYLGFGDDVQEHL
                     SQLSFGDAYQPDVFHAALANDKMIFVENALDPAFAPKLPRWWKDAFPTARSFMVLPLT
                     VNRRPAGFIYGDWDVERPSARIEQTEVAPLNELRTVIMRAMEQQRLFDAALLRNT"
BASE COUNT          780 a         1235 c         1340 g          784 t
ORIGIN      
        1 agcccttgct gaccaggtgc aggtgaatcg cggacgtcgc gagcagcgcc gggatggcga
       61 cgtgctcggc gtcgaacttg cggtcagtga tgatcaggat gttgtggccc gacctgaccg
      121 cgtcgaccgc ctttgcgcac agcgaggcca ggcgcgcctc gatgccttcc ttgccccagg
      181 cgacggggta gcaaatgttc agctcatgcg acttgaactt gccgccagtg tgcttgctga
      241 tgttgcgcag cttcgccatg tcggcatagt cgaggatagg ctgcgcgacc tccagccgca
      301 tcggcggatt gatgttgttg gtgtcgagca ggttcggctt gggtccgatg aaggatacca
      361 gcgacatcac catcgcctcg cggatcgggt cgatcggcgg gttggtcacc tgggcgaaca
      421 actgcttgaa gtagttgtag agcgtcttgt tcttgtttga catcaccgct agcggcgagt
      481 cgttgcccat cgagccgacc gcctcttcac cgttggcagc catcggcgcc atcaggaact
      541 tgatgtcttc ctgggtgtag ccgaatacct gctggcggtc cagcagcggg agctcggact
      601 tcggctcgac cggctccgcg tcgagctcat cgagcttgat gcgcacggaa tcgatccact
      661 gcttgtacgg ctttgcgttg gcgtaggtgt ccttcagttc cttgtcgtcg atgatgcgac
      721 cggcatcgag atcgatcagg aacatcttgc cgggctgcag acgccacttc ttgatgatct
      781 tcgattcggg gatcggcagt acgccggatt ccgacgccat caccaccagg tcgtcgtcgg
      841 tgatgatgta gcgcgccgga cgcaggccat tgcggtcgag cgtgcctccg atgtgacggc
      901 cgtcggtgaa ggccatcgcg gccgggccgt cccatggctc catcatcgcg gcgtggtact
      961 cgtagaacgc gcggcggttg tcgtccatca gcgtgtggtt ttcccacgct tccggaatca
     1021 tcatcatcat cgcctgcgcg accgggtagc cggccatcac cagcagttcg agcgcgttgt
     1081 cgaagcaggc ggtgtcggac tggccttcat agatcagcgg gaacagtttc tgcaggtctt
     1141 cgccgagcac ggccgacttc atcacgcctt cgcgtgcgcg aatccagttg aagttgccct
     1201 tgacggtgtt gatctcgccg ttgtgggcga cgtagcggta ggggtgggcc agcggccact
     1261 ccgggaaggt gttggtcgag aagcgctggt gcacgagggc cagcgcggac acgcagcgcg
     1321 gatcctgcag gtcctcgtaa tagacgccga cctggtcggc cagcagcaag cccttgtaga
     1381 cgacggtgcg cgccgacatc gacggcacga aaaactcctt gccatggatc aggttgagcg
     1441 cctggatcgc gtggccggaa gatttgcgga tcacgtacag cttgcgctcg agggcgtcgg
     1501 tcaccatgat gtccggaccg cggccgataa agatctggcg gatcaccggc tccttggcgc
     1561 gtacggtcgg cgacatcggc atctcgcggt cgaccggtac atcgcgccag ccgagcacga
     1621 cctggccctc ggccagtacc gcgcgctcga tctcctgctc gcaggcgatg cgcgaagcgt
     1681 tttccttcgg caggaacacc atgccgacgc cgtattcacc gggaggagga agggctacgc
     1741 cctgctttgc catttcttca cggtaatact ggtcgggaat ctggatcagg atgcccgcac
     1801 cgtctcccat cagcttgtcc gcacccacag cgccgcgatg atccaggttc ttcaggatca
     1861 gcaggccttg ctcgatgatg gaatggcttt tcttgccttt gatgtgggcg atgaaaccga
     1921 cgccgcaggc gtcgtgctca ttggaaggat cgtaaagacc ttgcgcgcgc atggaattct
     1981 ccgcaacttg gatggtgtca ggagcgaaga atagtgcagc gcaatagata cttcaaacaa
     2041 aattaaacgg ggtcagagtc gaattgtaat ttcgcaccag aattgtgcaa tttaattggg
     2101 gacggagtca tcggcagtaa agagccgtca gctttttcgc tctgcctcgg ccttgcgcgc
     2161 cggccgccct cgacgagagg ggctgacacg ccggcccagg cgacgcgcga gatcctgctg
     2221 aaagctgttc gggccgaggg gccaacccct tcgcactgca ccattgagca tgccaacctc
     2281 gtcggccgac aggttctgat cgaccagcgc gcggtaggcg gcctcgcgag cgaaaggggt
     2341 gttaccaagc gcccaatagg cggcgtggtc ggtgatcagc gcatccggct tcagtcctgc
     2401 atgatgtgag gcgctcgacc aggggtagtc ggccacactg agggcaagtc ctgcacgcac
     2461 cggcagctgt tcgatgtacc ggctgcaaag cagcaggtag ctcgaggcat ccacgacggt
     2521 cgccttgtag cgcccctgcc agagcgtgcc ctggcgcgca taacgggcgt tgaaatacgg
     2581 cacatacttg cggccgatcc actgcatcat tcgccccagc ccatgctcgt cgtggggggt
     2641 ggcaagcagc tggaagtggt cgggcatgac cacgtaggca tggaccgata ccttgaactg
     2701 gcgtgcggct tcgcgcagcc agccgtggaa gatctggaag tcctgggcat cacgaaagat
     2761 ggtcaggctg tccagcccgc tctgggtgac gtgatggggt tgattcggcg cgacgagccg
     2821 cggtagacgt gccatgacag aggagctgtc gatccgttgc aggagggagc cccgattcta
     2881 gcccggatag ctcaccgaac cgtcgctcag ttcggcagga aggcggacgt cagcgggatt
     2941 tgcgcatgca gcagccaggg ccacttcgcc gcatgcggaa gaaacagcgg gcctggcgcc
     3001 gaagccggct gcgctcagct gtgcgcgttg agcgtattgc gccagatggc cgcgcgcggc
     3061 ttgaactcga tcggcgcggc gtccggcatc gactgctcgg cgcactgcac ggtcgccgcc
     3121 gcgcgaggaa acacgctgga acggatcttg gaaacggaag gcatctgctg ctggcggaaa
     3181 cgcagcaccg gaacgccggc ttcactgaaa tagctgtcgc attccgcctc gcgccgtgtc
     3241 agccggggtc gatgatccaa ttcaatcacc gcaatgagtt gcatttcttc gtcgaacacc
     3301 gcgtagccaa cccggcgccc gacgatgcgg gagagggccg cctttcgtgc gcggccgacg
     3361 ccgagcggct cgagcagaga agtggctgcc acttggggac ataccgtgca ttctggtaat
     3421 gcccgtttga gacggaacag gaattccagc gcacttccgg tcagaatggg acgcgccctg
     3481 aaacgcgcgg gccttggccg gttcagaatg aaccagccca ataggcccga tccgagaatc
     3541 acgagaaaga cgccgagctc ggccatagcc cctccctgtg tagaaaaagg caactaatgt
     3601 aacacttgca acagggaggt gtgaagaaag ttacaatttt gccggcgctc ccggcgagct
     3661 cgggtcaggt atttctcagc agagcggcgt cgaacagccg ctgctgttcc atcgcgcgca
     3721 tgatcacggt ccgcagttca ttgagcggag caacctccgt ctgctcgatg cgagccgatg
     3781 gacgttcgac gtcccagtcg ccatagatga agccggccgg acgccggttg accgtgagcg
     3841 ggaggaccat gaagctgcgc gcagtcggga aggcatcctt ccaccagcgc ggcagcttcg
     3901 gcgcgaaggc gggatcgagc gcgttttcca cgaagatcat cttgtcgttc gcaagcgccg
     3961 catggaagac atccggctgg taggcgtcgc cgaaggacaa ttgggacagg tgctcctgca
     4021 cgtcgtcgcc gaagccgaga taggcgacgt atcgcaggcg ctcgggccgt cgcaggaaca
     4081 ccaggcatcg gctgaagcgc agaccctgat aaaccgtctc cagccccatg gtcagcagt
//
LOCUS       DATLGF010000495         5064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_203133, whole genome shotgun sequence.
ACCESSION   DATLGF010000495 DATLGF010000000
VERSION     DATLGF010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203133"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VM406_15365"
     CDS             <1..501
                     /locus_tag="VM406_15365"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVL77392.1"
                     /translation="RVTSPNAGVLGIGRGVLSVVRAIPDILYALLFVAAVGVGPLPGI
                     AALILFNIGVIAKLLSETVDGVDPGPIEAGRAAGATRAQTVRWGVLPQVMPNYIAYSL
                     YVFELNIRASTVLGIVGAGGIGGALFSAFQRFDYDFVLTILATIIAIIVLGELLANYV
                     KRAFHV"
     gene            494..1321
                     /gene="phnE"
                     /locus_tag="VM406_15370"
     CDS             494..1321
                     /gene="phnE"
                     /locus_tag="VM406_15370"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HVL77393.1"
                     /translation="MSDATLAGASAPIERRTWQRHTPAQRLFRFSVWLVIAVAVVQSL
                     RTIEVIPEFLYDAPEQMVDMMERMWPVDWAHYPSGVHPALVETLHIATLGTLLSIVLA
                     IPFGLLVAGNLTRSKPLNLLARIVLVSSRSVNSLVWALLFVAVFGPGAVAGTLAIAFR
                     SIGFVGKLIGEAIEEMPRGPIEALQAAGASKAAEIWYGYWPQLKPAFWSIVLLRWDIN
                     VRESAVLGIVGAGGIGMALDTAINLFQWDRVAMVLIAIFAVVIVAEIVVTQIRKRVL"
     gene            1321..2163
                     /locus_tag="VM406_15375"
     CDS             1321..2163
                     /locus_tag="VM406_15375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HVL77394.1"
                     /translation="MRALTEWPLEARRATRGVLTDIDDTLTTHGQLTPAVLDALSRLR
                     AVGFLLVAVTGRPTYWALPLLRLCGLHAVIAENGASAFWLDAAGRQQALFYADMQTRR
                     RHREELEAFAGMLSRRFPEVTVADDAAMRVGDLAFDIGETIAPLPPERVEQVLGFIRA
                     HGFHATASSIHAHASKAPFSKQAMSERVLREVFGIADDDARATFVFVGDSGNDASMFA
                     HYPHSIGVANIARYLAVLPVAPAYLTREASGAGFVELAQCLLEARASEPDCAYGRGES
                     AARA"
     gene            complement(2146..2691)
                     /locus_tag="VM406_15380"
     CDS             complement(2146..2691)
                     /locus_tag="VM406_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HVL77395.1"
                     /translation="MALKATIFKADLQIADMDRHYYADHTLTIARHPSETDERMMVRL
                     LAFALNADEALAFGRGLSTEDEPDLWRKDLTGAITTWIDVGLPDERRITKACGRSEQV
                     LVYTYGSTAGVWWKQVGPRLERLDKVRVMHIEPAQTVALAALAERTMRLQCTIQDAQV
                     WLTGARGAVEISPVTLSGARG"
     gene            2811..3386
                     /locus_tag="VM406_15385"
     CDS             2811..3386
                     /locus_tag="VM406_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HVL77396.1"
                     /translation="MSGTPSREAMEQEIVERTRHWLEAAVIGLNLCPFAKSVYVKNQV
                     RFAVSFAEDAAALHQDLIRELQFLQSSDPEALDTTLLIHPFVLNDFLDYNDFLDIADA
                     AVVDLELDGVLQVASFHPDYQFADTDADDITNFTNRAPYPILHLLRESSVERAVEAFP
                     EASSIFERNMETMRSLGRQGWETLMESRKAS"
     gene            3620..4015
                     /locus_tag="VM406_15390"
     CDS             3620..4015
                     /locus_tag="VM406_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77397.1"
                     /translation="MTTQSGNNMTSNRLADQVNYDPNNLLDALIEKLNLKNDAALSRA
                     LEVAPPVISKIRHRRLPVGASLLIRMHEVSDLSIKELRTLMGDRRDKFRISDKQFKPK
                     APTAGATGLAAGGETAAQLDDPGAETGTE"
     gene            complement(4129..5031)
                     /gene="cysM"
                     /locus_tag="VM406_15395"
     CDS             complement(4129..5031)
                     /gene="cysM"
                     /locus_tag="VM406_15395"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HVL77398.1"
                     /translation="MEYLTIEDTIGNTPLVQLKRLPGEAAARRNNVILGKLEGNNPAG
                     SVKDRPALSMIKHAEARGEIKPGDTLIEATSGNTGIALAMAAAMRGYKMVLLMPENLS
                     EERRQSMAAYGAQIVLTPKAGGMEYARDLATQMQKEGKGIILDQFANPDNPRAHYETT
                     GPEIWRDTEGRVTHFVSAMGTTGTIVGVSRYLKEQNPAVRIIGAQPEEGSQIPGIRKW
                     PEAYLPKIFDKSCVDQIEQVSQAAAEQMARRMAVEEGIFCGISAAGACEVALRISETV
                     ENATIVFIVCDRGDRYLSTGVFPA"
BASE COUNT          873 a         1654 c         1605 g          932 t
ORIGIN      
        1 cgtgtgacca gccccaacgc cggcgtgctg ggcatcggcc gaggcgtgct gagcgtcgtc
       61 cgcgcgatcc ccgacatcct ctatgcgctg ctcttcgtcg ccgccgtcgg tgtcggtccg
      121 ctgcccggca ttgccgcgct catcctcttc aacatcgggg tcatcgccaa gctgctgtcg
      181 gagacggtcg acggcgtcga cccgggcccg atcgaggccg gcagggccgc cggcgcgacg
      241 cgcgcgcaga ccgtccggtg gggggtgttg ccgcaggtga tgcccaacta catcgcctat
      301 tcgctctacg tcttcgagct caacatccgc gcctcgacgg tgctcggcat cgtcggcgcc
      361 ggcggcatcg gcggcgcgct gttctccgcg ttccagcgct tcgactacga cttcgtcctg
      421 acgatactcg caaccatcat cgcgatcatc gtgctcggcg aactgctcgc caattacgtg
      481 aaaagggcat tccatgtctg acgccacgct tgccggcgcc agcgcaccga tcgagcggcg
      541 cacctggcag cgccacacgc cggcccaacg gctgttccgc ttctccgtct ggctcgtgat
      601 cgcggtggct gtcgtgcagt cgctgcgcac catcgaagtc atcccggaat tcctgtacga
      661 cgccccggaa cagatggtcg acatgatgga gcgcatgtgg ccggtggact gggcgcacta
      721 cccgagcggc gtgcatccgg ccttggttga gacgctccat atcgccacgc tgggcacgct
      781 gctgtcgatc gtgctggcga ttccattcgg cctgctggtg gccggcaatc tcacgcgtag
      841 caagccgctg aacctgctcg cgcgcatcgt gctcgtgtca agccgctcgg tgaactcgct
      901 ggtgtgggcg ctgctgttcg ttgcggtgtt cggccccggc gcggtagccg gcacgctggc
      961 gatcgcgttc cgttcgatcg gcttcgtcgg caagctgatc ggcgaggcga tcgaagaaat
     1021 gccgcgcgga ccgatcgagg cgctgcaggc ggccggcgcg agcaaggcgg ccgagatctg
     1081 gtacggctac tggccgcagc tcaagccggc attctggtcg atcgtgctgc tgcgctggga
     1141 catcaatgtg cgtgaatctg cggtgctggg catcgtcggc gccggcggga tcggcatggc
     1201 gctggatacc gcgatcaacc tgttccagtg ggatcgcgtg gcgatggtgc tgatcgcaat
     1261 tttcgcggtg gtgatcgtgg cggagatcgt cgtcacccag atccgcaagc gcgtgctctg
     1321 atgcgggcgc tgaccgaatg gccgctcgag gcgcggcgcg ccacgcgcgg cgtgctgacc
     1381 gacatcgacg acaccctgac cacgcacggc cagctgacgc ccgcggtgct ggacgccctc
     1441 tcccgcctgc gcgccgtcgg ctttctgctc gtcgccgtca ccggccgccc gacctactgg
     1501 gcactgccgc tcttgcgtct gtgcgggctg catgcggtca tcgccgaaaa cggcgcgagc
     1561 gcgttctggc ttgatgccgc cggaagacag caggccctct tctacgcgga tatgcagacc
     1621 cggcgccggc accgggagga gctggaagcg tttgctggca tgctgagccg tcgcttcccg
     1681 gaagtgacgg tggccgatga cgcggcaatg cgcgtcggcg atctggcctt cgatatcggc
     1741 gagacgatcg caccgctgcc gccagaacgt gtcgagcagg tgctggggtt catccgcgcc
     1801 cacggctttc atgcaacggc gagcagcatc cacgcgcacg cgtcgaaagc gccgttctcc
     1861 aagcaggcga tgagcgaacg cgtattgcgc gaggtgttcg gcattgccga cgacgacgcc
     1921 cgggcaacgt tcgtcttcgt cggcgattcg ggcaatgatg cgtcgatgtt cgcgcactat
     1981 ccgcactcga tcggcgtcgc caatatcgcg cgttaccttg cagtcctgcc ggtcgcaccg
     2041 gcctacctca cccgcgaagc ctccggcgcc ggctttgtgg aactcgcgca gtgcctgctc
     2101 gaggcgcgcg catccgagcc ggactgcgcg tacgggcgcg gcgaatcagc cgcgcgcgcc
     2161 tgacagcgtc acgggcgaaa tctccaccgc gcccctcgct ccggtcaacc acacctgcgc
     2221 atcctggatc gtgcattgca gccgcatcgt gcgctcggcg agggcggcca gcgccacggt
     2281 ctgggccggc tcgatgtgca tgacgcgcac cttgtccagg cgctcgagcc gtggccccac
     2341 ctgcttccac catacgccgg cggtcgaccc gtaggtgtat accagcacct gttcggaccg
     2401 gccgcaggcc ttcgtgatcc tccgctcgtc cgggaggccc acgtcgatcc acgtcgtgat
     2461 ggcgccggtc aggtccttgc gccacagatc cggctcgtcc tcggtgctca gcccgcgccc
     2521 gaaggcaagc gcctcatcgg cattcagcgc aaacgccagc agtcttacca tcatgcgttc
     2581 gtcggtttcg gacgggtggc gggcgatggt cagcgtgtga tcggcgtaat agtgccggtc
     2641 catgtcggcg atctgcagat cggccttgaa gatggttgcc ttgagggcca tataggaaaa
     2701 atcgggggaa gagtcgtatc gaatgggcga agaggcgcaa gtgtagcgca agccggcaca
     2761 cctccggccg gcagcgccca gccgcagcca ctacccacaa aggaagcaga atgagcggaa
     2821 caccgagccg cgaggcaatg gagcaggaaa tcgtggagcg gacgcggcac tggctggagg
     2881 cggccgtcat cggcctgaac ctttgccctt ttgccaagtc ggtctatgtg aagaaccagg
     2941 tacgcttcgc ggtcagtttc gccgaggacg cggccgcgct gcatcaggac ctgatacggg
     3001 aattgcagtt tctgcaatcc tccgatcccg aggcattgga caccacgctg ttgatccacc
     3061 ctttcgtgct gaacgatttc ctcgactaca acgatttcct cgatatcgct gacgctgccg
     3121 tggtggatct ggaactggac ggggtgctgc aggtggcgag cttccatccc gattaccagt
     3181 tcgccgacac ggatgccgac gacatcacga acttcaccaa ccgcgcgccc tatccgatcc
     3241 tgcacctgct gcgcgaatcc agcgtcgagc gcgcggtcga agcctttccg gaagcaagca
     3301 gcattttcga gcgcaacatg gaaaccatgc gttcgctggg aaggcaggga tgggagacgc
     3361 tgatggaaag ccgtaaagcc tcctgatacc cctccggggg ccgttgcggg ggctcaagtc
     3421 cgcgcgcccg cctctcgcta gaatgcggga ttcggcgccc gcaaccgcgc cgcagcatgg
     3481 ccgagtgttg ccattccagt tactcgaaaa agcgagaaat tggcgggcgg ctccgggtga
     3541 aaggggaagt aacagttttt cgaaagaatt cggcgaactg ctctgtacaa cgaacttgca
     3601 gtatggaatt attgagacta tgactactca atcaggcaat aacatgacca gcaaccgact
     3661 cgccgatcaa gtcaattacg atcccaataa cctgctcgat gccttgatcg aaaaactcaa
     3721 tctgaagaac gacgcggcgc tgtcgcgcgc gctcgaggtc gcgcctccag tcatcagcaa
     3781 gatccgtcat cgccgcctgc cggttggagc gtcgctgttg atccgcatgc acgaagtcag
     3841 cgacctgagc atcaaggaat tgcgcacgct gatgggcgat cgccgcgaca agttccggat
     3901 cagcgacaag cagttcaaac cgaaggcgcc gacggcaggt gctacaggcc tggcagcagg
     3961 cggcgagacc gcagcacagc tggacgatcc gggagccgaa accggcaccg agtaagttgc
     4021 cacccaagaa gccctaacaa aacgccgatg gcaaggcgcg ccgacgcaga cagtacgtgt
     4081 agtacggcaa ggagccgcaa cgctgcgagc ggattcttgt tagggccact aagcgggaaa
     4141 cacgccggtc gacagataac ggtcgccacg atcgcagacg atgaacacga tcgtggcgtt
     4201 ctcgacggtt tccgaaatgc gcagcgccac ctcgcacgcg cccgcggctg aaatgccgca
     4261 gaaaatcccc tcctccaccg ccatgcgccg cgccatctgc tccgccgcag cctggctgac
     4321 ctgctcgatc tggtccacgc aggatttgtc gaagatcttc ggcaaatagg cttccggcca
     4381 tttgcgaatg cccgggatct gcgagccttc ctcgggctgg gcgccaatga tgcgcaccgc
     4441 cggattctgc tctttcaggt agcgggaaac gcccacgata gtgccggtgg tgcccatcgc
     4501 gctgacgaag tgggtcacac gtccctcggt atcgcgccag atctccgggc cggtggtttc
     4561 gtagtgcgcg cggggattat caggattggc gaactggtcg aggatgatgc ccttgccttc
     4621 cttttgcatc tgcgtggcga gatcgcgcgc atattccatc ccgccggcct tgggtgtgag
     4681 cacgatctgc gcgccgtagg ccgccatgct ctggcgacgc tcctcgctca ggttctccgg
     4741 catcagcagc accatcttgt agccgcgcat cgccgccgcc atcgccagcg cgatgccggt
     4801 gttgccgctg gtggcttcga tcagcgtatc gccgggcttg atctcgccgc gcgcttccgc
     4861 atgcttgatc atcgacagcg ccggcctgtc cttcacggag cctgccggat tgtttccctc
     4921 cagcttgccg agaatgacgt tgttgcgccg cgccgcagct tcgccgggga gccgcttgag
     4981 ctgcaccaac ggcgtattgc cgatcgtatc ttcgatggtg aggtattcca ttgcctgccg
     5041 tctctgctgg gaaaggcatc attg
//
LOCUS       DATLGF010000496         4905 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_185728, whole genome shotgun sequence.
ACCESSION   DATLGF010000496 DATLGF010000000
VERSION     DATLGF010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185728"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VM406_15400"
     CDS             complement(<1..229)
                     /locus_tag="VM406_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77399.1"
                     /translation="MNAPLTSGQRALLENISLDDKFTLERGRAFITGTQALVRLPMMQ
                     RQRDVRAGLNTAGFITGYRGSPLGNVDMTAMK"
     gene            516..818
                     /locus_tag="VM406_15405"
     CDS             516..818
                     /locus_tag="VM406_15405"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HVL77400.1"
                     /translation="MRSTILLMIASATFGSSLAIAQSALVHVPTEKKVPHALSKFTER
                     FMAADKNGDGALSREEAQAAGLHRIVDNFDRLDADGDGKVTLDEVRTLLKQQRPTI"
     gene            complement(977..1861)
                     /gene="phhA"
                     /locus_tag="VM406_15410"
     CDS             complement(977..1861)
                     /gene="phhA"
                     /locus_tag="VM406_15410"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HVL77401.1"
                     /translation="MNTTVEQADFFKTLEEKSDGGKLRGDYSQCDARYVVKQNWETYT
                     PEQHELWRRLYQRQAKLLPGRACDVFIESLRKMDASAEIPRFERTSEALYKATGWEIV
                     AVPGLVPELTFFEHLANRRFPVTVWLRDPEEFDYIVEPDVFHDFFGHVPLLFNPVFAD
                     YMQQYGQGGLKALELGGLDQLARLYWYTVEFGLIRSEQGLRVYGAGILSSGGEIEYCL
                     TSPQPRRIALQAERTMRTLYKIDSYQETYFVIDDFQQLFDQTAPDFTPIYERLKQQPV
                     LAANTLLPGEINIAPNSR"
     gene            2035..2526
                     /locus_tag="VM406_15415"
     CDS             2035..2526
                     /locus_tag="VM406_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HVL77402.1"
                     /translation="MPRINLDKTDRRILAILQENGRLSNQEIADRVSLSPSPCLRRIR
                     NLEDLGVIRQYVALLDPEKIGLGLLAYVNVRLEKHSDAAAGGQRSPRADFAAAVAAWP
                     EVVACYAMTGEMDFLLRVHVEDMEHFSHFMMDTLLKHSAVLDVKSSFALQRIKDTTAL
                     PLV"
     gene            complement(2537..3163)
                     /locus_tag="VM406_15420"
     CDS             complement(2537..3163)
                     /locus_tag="VM406_15420"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVL77403.1"
                     /translation="MTRRPAAPAVERNREQTTARILAAVGEVLARDGFAALGVNAIAK
                     EAGVDKVLIYRYFGGLPELLAAWAQSGRFWPGVDEILGADPDAFFALSAPERFARFFD
                     LFIDSLRQRPITLEIMVAELVERNELTAVLEAEREAWGQRAVQMLGGEAFAARPDLQG
                     LALLLVAGVQYLVLRSRKIRIYGGVDIQSDEGWNALKSSLRAAAYRLF"
     gene            complement(3160..4326)
                     /locus_tag="VM406_15425"
     CDS             complement(3160..4326)
                     /locus_tag="VM406_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation pathway signal
                     protein"
                     /protein_id="HVL77404.1"
                     /translation="MNRRNFIRVAGGGVVLAAGGLAGCSTTYPEEAVAAWRGPSPSAD
                     VRRWALGYAILAPNSHNLQPWLVDLRDPDAIVLHVDRERLLPMTDPWYRQIMVSQGTF
                     LEALAIALRQRGLEPKFELFPQGEPGRFELDGRPVARITWQPGAAPDPLFAQLLKRHT
                     AKGGYDTGARVDPRVLAALAESLQDNAVQYGYTVEREKLSALSRLALDASKVELDTPR
                     TILESVRLTRVGPAEIARHRDGISVNNRIARLADMLGAFDRDAPLQPGSAGYRQTLAQ
                     FEEQTQSAMGFVWLSTATAQHLRAGAVRSAEVRAGRAYMRLQLKATELGLQMHPMSQA
                     LQEFDEMRPHYMRLHRLLLGAQPEQQTLQMFCRVGYGRPSQPAPRRGVDAIIRA"
     gene            complement(4405..>4905)
                     /locus_tag="VM406_15430"
     CDS             complement(4405..>4905)
                     /locus_tag="VM406_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL77405.1"
                     /translation="VITRYVQKLDFARDSVFGIYDQDFRLVGVGHLAFLPREAIAVLR
                     EASEKQQVAELGVSVLPEVRGEGIGTRLFVRAAIHCRNRDVDTLHMKCLARNEAMMHL
                     ARKAGMEIHVGCGEANAYLKLRLADPLSVLREAIEEQYAALDYTRQSNFHRPRRRCRA
                     LPGEPE"
BASE COUNT          874 a         1618 c         1522 g          891 t
ORIGIN      
        1 ccttcatcgc agtcatgtcc acattgccga gtggggagcc gcggtagccg gtaatgaagc
       61 cggcggtgtt gagcccggcg cgcacgtcac gctgacgctg catcatcggc aggcgtacca
      121 gcgcctgtgt cccggtgatg aacgcgcggc cgcgctcgag ggtgaacttg tcgtccaggg
      181 aaatgttttc cagcagcgcg cgctgcccgg aagtcagagg tgcgttcaat tcagtctcca
      241 gccaaaaatt ttgtctttgg gtgcgatgca ttcgctgtca aacgggcaag ccttgtgagc
      301 ccaccgtcgc tctaattttg acagtctgcc cactagcata cgcgtgcttt tctctccatg
      361 cacggcgcag cacgcgccat gctgtcgggg gcggttcgga ggtgcgtcgg caggtgctgt
      421 aacagcgcgt aaatgccatg gtgcaagact ctgccgctcc ttagagtcgt tctcaggtgc
      481 cgcgcccgcg acgcctgttt cctcgagttc ccgccatgcg ctccaccatc ctgctcatga
      541 tcgcttccgc caccttcggc agcagccttg cgatcgccca gtcggcactg gtccacgttc
      601 ctacggaaaa gaaggttccg catgcgctgt caaagttcac ggaacgcttc atggcggcag
      661 acaagaatgg cgacggtgcg ctatcccgcg aagaggctca ggccgcgggc ctgcaccgca
      721 tcgtcgataa cttcgaccgg ctcgacgccg acggcgacgg caaggtcacg cttgacgaag
      781 tgcgcacgct gctcaagcag cagcgcccga cgatctaagg ctacgctgaa caagtcctcg
      841 cgcgtcgcgc atcccgaact cgggcggcct gcggcgttgc gaatactcgc gatagctcca
      901 ctatcgctgc gtttcgcgcc ttgcatccca tcccgatttc gggcgcgcgc catcgcgggg
      961 cttatccagc gtagccttac cgggaattcg gcgcgatgtt gatctcgccc ggcagcagcg
     1021 tgttcgccgc cagcaccggc tgctgcttca agcgctcgta gatcggcgtg aagtccggcg
     1081 cggtctggtc gaacagctgc tggaagtcgt cgatcacgaa ataggtctcc tggtacgaat
     1141 cgatcttgta cagcgtgcgc atcgtgcgct cggcctgcag cgcaatgcgg cgcggctgcg
     1201 ggctggtcag gcaatactcg atttcgccac cggacgacag gatgccggcg ccatacacgc
     1261 gcagcccctg ctcggagcgg atcagcccga actccaccgt gtaccagtac aagcgcgcca
     1321 actgatccag cccgcccagt tccagcgcct tcagcccgcc ctgcccgtac tgctgcatgt
     1381 agtcggcaaa caccggattg aacagcagcg gcacatgacc gaagaaatcg tggaagacgt
     1441 ccggttcgac gatgtagtcg aactcctcgg ggtcgcgcag ccagacggtc accgggaaac
     1501 ggcggttggc caggtgctcg aagaaggtga gttccggaac caggcccggc accgcgacga
     1561 tctcccagcc ggtcgccttg tagagcgctt ccgaggtgcg ctcgaaacgc gggatctcgg
     1621 ccgacgcgtc catcttccgc aggctctcga tgaagacatc gcaggcgcgg cccggcagca
     1681 gcttcgcctg gcgctggtac aggcggcgcc acagctcatg ctgttcaggg gtgtaggtct
     1741 cccagttctg cttgacgacg taacgcgcgt cgcactggct gtaatcgccg cgcagcttgc
     1801 caccgtcgga cttttcctcc agcgtcttga aaaaatcggc ttgttctacc gtggtgttca
     1861 tggctgtctc caactcttta ctgtgatgga tctcctttcg gagctcgctc ggagatcgaa
     1921 aataaagctc aaaacagtat agacagacag gcacgacatt aaattgccaa aactgcccgc
     1981 gaataccgga atttggcaat atcattgcgc gtacactcgc ttcggagcct gtttatgcca
     2041 agaatcaacc tcgacaagac cgatcgcagg attcttgcga tcctgcagga gaacgggcgc
     2101 ctgtccaacc aggagatcgc cgaccgcgtc tcgctgtcgc cttcgccctg cctgcgccgc
     2161 atccgcaacc tggaagacct cggcgtaatc cggcagtacg tcgcgctgct cgatccggaa
     2221 aagatcgggc tgggcctgct ggcctacgtg aacgtgcgcc tggaaaagca cagcgacgcg
     2281 gcagccggcg ggcagcgctc gccgcgcgcg gacttcgccg ccgcggtggc ggcctggccg
     2341 gaggtcgtgg cctgctatgc aatgacgggg gagatggatt tcctgttgcg ggtgcatgtg
     2401 gaagacatgg agcatttctc ccacttcatg atggacacgc tgctcaagca ttcggcggtg
     2461 ctggatgtga agtcgagctt cgcgctgcag cgcatcaagg acacgaccgc gctgccgctg
     2521 gtgtgagcat gcatcgtcag aacaggcggt aggccgcagc gcgcagcgag gatttcaacg
     2581 cattccagcc ctcgtcgctc tggatgtcca caccgccgta gatgcggatc tttcggctgc
     2641 gcaggaccag gtactgcacg ccagcgacca gcagcagcgc caggccctgc aggtccggcc
     2701 gcgcggcgaa ggcttccccg ccgagcatct gcacggcgcg ctgcccccac gcctcgcgct
     2761 cggcttcgag caccgcagtc agctcgttgc gctcgaccag ttcggccacc atgatctcca
     2821 gcgtgatcgg gcgctggcgc aggctgtcga tgaacaggtc gaagaaccgg gcgaatcgct
     2881 ccggcgcgga cagtgcaaag aaagcgtcgg ggtccgcccc caggatctcg tccacgccgg
     2941 gccagaagcg cccgctttgc gcccatgcgg caagcagttc gggcaggccg ccgaagtagc
     3001 ggtagatcag taccttgtcg acccccgcct ccttggcaat ggcattgacg cccagtgcgg
     3061 caaagccgtc gcgcgccagc acctcgccga cggcggccag gatgcgcgcg gtggtctgtt
     3121 cacgattgcg ttcgacggca ggggcagcgg ggcggcgagt cacgcgcgga tgatagcatc
     3181 cacccctcgt cgcggcgccg gctgggacgg cctgccatag cccacccggc agaacatctg
     3241 caacgtttgc tgttccggct gcgcgccgag cagcagccgg tgcaacctca tgtagtgcgg
     3301 ccgcatctcg tcgaattcct gcagcgcctg gctcatggga tgcatctgca gacccagttc
     3361 ggtcgccttg agctgcaggc gcatgtaggc acgcccggcc ctgacctcgg cactgcgcac
     3421 cgcgccggcg cgcagatgtt gcgcggtcgc ggtggagagc cagacgaatc ccatcgcgct
     3481 ttgcgtttgc tcctcgaatt gcgccagcgt ctggcggtag ccggcgctgc ccggctgcaa
     3541 aggcgcgtcg cgatcgaatg cccccagcat gtcggcaagc cgcgcgatgc ggttgttgac
     3601 gctgattccg tcgcggtgcc gcgcgatttc ggcaggcccc acgcgcgtca ggcgcacgct
     3661 ttccagtatg gtgcgcggcg tgtccagctc caccttcgag gcatcgaggg cgagacgact
     3721 cagggccgaa agcttttccc gttccaccgt gtagccatat tggaccgcat tgtcctgcag
     3781 tgactcggcc agcgccgcca gcacgcgcgg gtcgacgcgc gcgcccgtgt cataccctcc
     3841 cttggccgta tgtcttttca gcagttgggc aaacaggggg tccggcgccg cgccgggctg
     3901 ccacgtgatg cgtgcgaccg gccggccatc gagctcgaag cgacccggct cgccctgtgg
     3961 aaacagttcg aatttaggct ccaggccgcg ctggcgcagc gcgatggcga gcgcttcaag
     4021 gaacgtgccc tggctgacca tgatctggcg ataccacgga tccgtcatcg gcagcaggcg
     4081 ttccctgtcg acatgaagaa cgatagcgtc cgggtcgcgc agatcgacca gccatggctg
     4141 caggttatgc gagttcggcg cgagaatcgc gtagccgagc gcccaccgcc gcacatcagc
     4201 ggagggactg gggccgcgcc atgcggcgac cgcttcctct ggataggtcg ttgaacagcc
     4261 cgcaagcccc cctgcggcca ggaccacacc tccaccagcc acccgaatga agtttctgcg
     4321 attcatgcca cccccttgtc actgtatagt gacatgatag tcaccgaatg gtgacatgtc
     4381 aaacgggaaa tcgcatgccc agacctattc cggttcgccg ggtaaggcgc ggcaccttct
     4441 gcgcgggcgg tggaagttgg actggcgggt atagtcaagc gctgcgtact gctcctcgat
     4501 cgcctcgcgc agcacgctca gaggatcggc gaggcgcagc ttcagatagg cgttggcctc
     4561 gccgcagccg acatgtatct ccatgccggc cttgcgggcg aggtgcatca tcgcctcgtt
     4621 gcgtgcaagg cacttcatat ggagcgtatc cacatcccgg ttgcggcagt ggatggcagc
     4681 acgcacaaac agccgcgttc cgattccctc gccgcgcacc tccggcagga ccgacacccc
     4741 gagttccgcc acctgctgct tctcgctcgc ctccctcagg accgcaatag cctcccgcgg
     4801 caggaacgcg agatggccga cgcctaccag cctgaagtct tggtcgtaga tgccgaacac
     4861 cgaatctcgc gcgaaatcca gtttctgcac atagcgcgtg atgac
//
LOCUS       DATLGF010000497        17804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_78792,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000497 DATLGF010000000
VERSION     DATLGF010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 17804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17804
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78792"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VM406_15435"
     CDS             <1..554
                     /locus_tag="VM406_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL77406.1"
                     /translation="PLPAVTRAFDIGTGTGVLAAVLARRGVEQVVATDQDPRALACAR
                     ENIARLGLGGQVQVMQADLFPPGRAMLAVCNPPWLPARPSSPIEYAVYDPDSRMLRGF
                     LNGLADHLEPGGEGWLILSDIAEHLGLRSRAELLAMIDGAGLTVLGRLDARPRHPKAG
                     DPDDPLYQARKKEVTSLWRLAAK"
     gene            complement(594..1514)
                     /locus_tag="VM406_15440"
     CDS             complement(594..1514)
                     /locus_tag="VM406_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL77407.1"
                     /translation="MKKLPDLEAWAIFAKVAETGSFARAAAELSVSQATVSKAITRLE
                     ARMKTVLLHRTPRRLSLTGSGNAALQRATRILEDGEAVEAEIAEQSNSLRGLIRVAAP
                     MSFGIERLSPLLPDFMSMHPDVALDIQFNDELVDLVANRFDLALRISRMTDSSLLARR
                     LCSVRVLLVGAPAYFARHGRPKHPHDLAKHRALLYTYTRGGTSWRFHHKRHGEFAQAM
                     PSNLQANNAEALVPALRAGLGLALQPEFLAWHDVQSGVLETVMDDWQVEPIALHIVTP
                     PGRNRPARVQALINYLAERFASEPWANLEG"
     gene            1669..2373
                     /locus_tag="VM406_15445"
     CDS             1669..2373
                     /locus_tag="VM406_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HVL77408.1"
                     /translation="MIERRPFASLGGAHHGWLDAKHHFSFAEYHDPARVHWGALRVWN
                     DDTIQPRTGFPPHPHADMEIITYVREGAITHQDNMGNKGRTVAGDVQVMSAGTGIRHS
                     EYNLEPDITRIFQIWIVPDRRGEPPSWGSKPFPKSDRSGRFSTLASGLEGDEDALPIR
                     ADARVLGATLKAGETAEYRFSGKGRHGYLVPAIGTVEINGITLEARDGAAIKDEEIVR
                     VTAREDAELVLVDTAA"
     gene            2520..3209
                     /locus_tag="VM406_15450"
     CDS             2520..3209
                     /locus_tag="VM406_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HVL77409.1"
                     /translation="MSTYLEVLTPQNSQLIFIDQQPQMAFGVQSIDRQLLKNNVVALA
                     KAAKVFNIPTTITTVETDGFSGNTFPELLAVFPENKLLERTSMNSWDDQNVRDALADG
                     AAQGRKKIVVSGLWTEVCNITFALSCLRDTDYTIYMVADASGGTSVDAHKYAMDRMVQ
                     AGVVPVTWQQVLLEWQRDWARRETYDAVLAIVKEHSGAYGMGVDYAYTMVHKAPERVR
                     HGERIGPNPAK"
     gene            3417..5312
                     /locus_tag="VM406_15455"
     CDS             3417..5312
                     /locus_tag="VM406_15455"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HVL77410.1"
                     /translation="MADSFPDIIFHHARITTLDRGNPVASAVAIKDGKFLAVGSDAQI
                     LALAGAGTRLVNLKHRCVLPGLFDNHTHVVRGGLNYNLELRWDGVRSLADAMEMLRRQ
                     VAITPAPQWVRVVGGFTEHQFVERRLPTIAEINTVAPDTPVFLLHLYDRALLNGAALR
                     AVGYTKDTPDPPGGEITRDAAGNPTGLLLAKPNALILYATLAKGPKLPFDYQVNSTRH
                     FMRELNRLGVTGVIDAGGGFQNYPEDYAVIQKLADEGQMTVRLAYNLFTQKPKAEKDD
                     FLKWTSSVRYKQGNDYFRHNGAGEMLVFSAADFEDFRQPRPDMPPQMEGELEEVVRIL
                     AQNKWPWRLHATYDETIARALDVFEKVNRDIPLQGLNWFFDHAETISDKSIDRIAALG
                     GGIAVQHRMAYQGEYFVERYGARAAEATPPVARVLEKGVKVSAGTDATRVASYNPWVA
                     LSWMVTGKTLGGLRLYPQRNCLDRETALRMLTENVAWFSNEEGRRGRIEPGQFADLIV
                     PAKDYFSVPEDEISFLASDLTVVGGRIVYGDGDFASLDDNPLPPAMPDWSPTRIFKGY
                     GAWGEPEGAGRHSLHPARYQAIASCGCASGCGLHGHDHARAWDAKLPVSDMRGFFGAL
                     GCSCWAV"
     gene            5341..6942
                     /locus_tag="VM406_15460"
     CDS             5341..6942
                     /locus_tag="VM406_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL77411.1"
                     /translation="MMQAPQTVSAPSSSFAPLRQRLFLVLWLATIIGNTGSFIRDVAS
                     AWLMTDLSPSPTAVALVQAAATLPVFLLAIPAGVLADILDRRKFLIAIQLLLASVSIS
                     LMLLSAFGLQSVTTLVAFTFLGGVGAALMAPTWQAIVPELVEKKDLKSAVGLNSLGVN
                     ISRAIGPAMGGLILASMGAAFTYGVDVLSYVFVVTVLLWWRRPSAAQDVLAERFAGAF
                     RAGLRYVRASRALHVVLARAFFFFALASSVWALLPLVARQLLGGGASFYGILLGAVGM
                     GAIVGAIIMPTLRQRLSADGLLLLAALVTALVMAFLSFAPPQWAAVVSLLMLGAAWIT
                     ALTTLNGVAQAILPNWVRGRSLAVYLTVFNGAMAAGSLVWGTVAERIGVPFTLLVAAA
                     GLVAVGLVAHRLKLPKGELDLVPSNHWPEPLTAAPVEHDRGPVLILIEYRIAAEDRGA
                     FLKALFELSAERRRDGAYAWGVSEDAADPGLMLEWFEVESWAEHLRQHHRVSKADADV
                     QAEVLSFHRGEGRPVVRHFLACTRP"
     gene            7099..7335
                     /locus_tag="VM406_15465"
     CDS             7099..7335
                     /locus_tag="VM406_15465"
                     /inference="COORDINATES: protein motif:HMM:NF021415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2116 family Zn-ribbon domain-containing
                     protein"
                     /protein_id="HVL77412.1"
                     /translation="MSDEADLAAETIELSRMAAIEACRRQPALPAKGSCWFCDEALPP
                     NQKFCDADCASDYELEQAAWARAGRGKPSDLYAD"
     gene            7438..8610
                     /locus_tag="VM406_15470"
     CDS             7438..8610
                     /locus_tag="VM406_15470"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVL77413.1"
                     /translation="MSSTSNAFLVGALFCVLMLLVLSSLFRSGLPGIRELFLANLLGG
                     IALVLYAFGREVPPLVGYEVANGLYAASAAAVLAGYRRFLGERVSWPLLIAGVGLVVA
                     AIALFHYRIDSFALRTTTIAVFQSALCIAIIVSIFAARHRWRSRYPYYFAAAMAGLIA
                     VAHITRSLVHLQRTDEITSLLQPSGWNLFFVSAGTFVLPVLTIALVMMVHDAMMARAE
                     HAANRDFLTGAWSRRAFFELGERELSRGRRHARRLSLLLLDIDHFKAINDNWGHAAGD
                     QVLIDTVARAGAVLRNGDFFARIGGEEFGVLLPETEGVEAERVAERLRAALDHTLIVH
                     QGRRKTASVSCTVSIGFAVQGRGETLHDILRRADAALYEAKQAGRNRVVAAADGGR"
     gene            complement(8676..11351)
                     /locus_tag="VM406_15475"
     CDS             complement(8676..11351)
                     /locus_tag="VM406_15475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HVL77414.1"
                     /translation="MENHYLTPLFAPRSIAVFAGDPNQSGNHPAHGRQIIAQLSDGGY
                     GGPVTFLDIGMRGTLSDLAHSRADLGIIALPNDQLQAALEVAARIQCKTVLIISSGVS
                     AAQAVQLYAIARRHGIHLLGPNSSGFQTPRLSLNASTLGPLAQQGPLGLISQSGALTT
                     AILDWAVKNRVGFSGVVSLGRDTALDLWQVLEYFAADRATQSILVYLEGIRDARRFMS
                     ALRNAANVKPVIVLKAGRKPAGSSAALTHSGTIVGSDDVFDAALQRAGAVRVRSFVQL
                     FSAAKCLASRYLPVGKRLAVISNGGGPGVLAADWANDLGLELATLPAAEAARLAPQLS
                     PLAQLESLIDLGEEADAAHYRAAVTACAASNAVDGMLVIQSPKPHLDTEAIAKALAEL
                     QPIGKPLITCWMGDERAAEPRKILARAAIPTFRTPEAAVDAFNNIASFHQNQQLLYQT
                     PPPMSPLAKPDVEGARILVESVLAERRKTLTEMESKALLSAFHIPITKTILTRSAGEA
                     ILIANQLGYPVALKIDSPDITHKSDIGGVALNLHNAAAVRDAWQDMMDTVARRCPDAK
                     LNGITIQNMSGKPQGRELYIGVASDEPFGPVITFGAGGTMVELIGDRAIELPPLNQFL
                     AQRLIERARAADTLGPWRGAPPAQREALEHILLRVSEMVCALPQLVAMDINPVIVDHN
                     GAIAVDARIVVGPAPGTPESYPHLAILPYPADFEQTWPMRGGGRYTVRAIHPDDAEML
                     QDFVRRMSPESRYNRFVSTMRELSVKMLARFTLIDYDREMALVAVLRERKPGRDGALV
                     DSERIIAVSRYVTNPDSVSCEFSLAVADEFCGQGIGSRMMLSIMEFARRKGLTRIEGL
                     VLTTNEPMLKLMRSLEFEITRYEEDPDFRLCVRQL"
     gene            complement(11418..12347)
                     /locus_tag="VM406_15480"
     CDS             complement(11418..12347)
                     /locus_tag="VM406_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HVL77415.1"
                     /translation="MLPAYISHTDCHKHEMGALHPECPQRLDAIHDHFLAKGLLDAMQ
                     AYDAPRATEAQLGRAHALLHVHEIAARVPPEDYAAIDPDTWMNPHTYDAALRAAGAAV
                     LATDLVLEGKAPVAFCNVRPPGHHAGRGFASGFCFFNNVVVGMRHALDVHNLERVALV
                     DFDVHHGNGSEDILCDDPRTLMCSTFERDLYPFSGTEPMGPNMVNIPLAARSGSEAFR
                     KAVQAHWLPALEAFRPQMIFVSAGFDAHRDDDLGNLALVEADYAWVTRTIMDIAHRHA
                     GGRVVSCLEGGYELHALARSAAAHVKVLIGVDA"
     gene            12835..13932
                     /locus_tag="VM406_15485"
     CDS             12835..13932
                     /locus_tag="VM406_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HVL77416.1"
                     /translation="MMFTTRDPISLTRTVLLGLFLVGLFILSYQVLRLFIVPVAWALI
                     LAYVTWPVYVHVRAAIGGSPTASAVVMTLLLTTAFVLPLLWLIGLLQNEFVLAFKAVS
                     TYLNDGPPLLPAFIRDIPWLGEFLQQLIERMAIDPEAMRKEMLQWAQGWVGHVTDLIG
                     GVGRNAAKFAMVVVTVLFVYRDGESLLKQVRKVSRRFLGARANAYLAAIGATTTAVLY
                     GLLLTAIAQGTLAGIGYWAAGIRAPALFGALTALIALLPFGAPFVWGTLSVWLLVQGE
                     TWAGIGLLLWGMLVVSWVDNLIRPLVISSATKIPFLLVMFAVLGGLAAFGLVGMFLGP
                     IVIAVLLAVWREWVDAEPPAPPPPPEEDTPV"
     gene            complement(13954..14292)
                     /locus_tag="VM406_15490"
     CDS             complement(13954..14292)
                     /locus_tag="VM406_15490"
                     /inference="COORDINATES: protein motif:HMM:NF022592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HVL77417.1"
                     /translation="MKGTMQILGMRGAVLPLPREDAVLELTRGQMLRLKNAKGTTIRV
                     VAGAVWVTQDHDARDLVLATGDDFTCDRHGLAVAVPLTDDGARLVLGDARTSARRALA
                     WLPLGRPRLA"
     gene            14419..15333
                     /locus_tag="VM406_15495"
     CDS             14419..15333
                     /locus_tag="VM406_15495"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL77418.1"
                     /translation="MDRLPEMMTFAKVVETHSFSAAALALGTSKSVVSKQVSALEAAL
                     GVRLLNRTTRRMSLTEVGAAYYEHCARIAREIEAAAQTVSQLQAEPRGVLRLTSPVIF
                     ASLHLAPALHSFMQRHEHVEVELNAVDRVVDIIEEGYDLAIRITGNPPQNMVARKISD
                     VRWATCAAPSYIARHGAPAIPQDLLRHACLTYEGIPALRGGDWRYRVAGRELSVRVGG
                     RVRVNNSEVLLQMALDGAGIVLFPTYIVGPHLQSGRLVEVLRDSVANPDMALVATYPP
                     NRYMQPKVRAFIDHLLAHFGPQPYWDRY"
     gene            complement(15335..15712)
                     /locus_tag="VM406_15500"
     CDS             complement(15335..15712)
                     /locus_tag="VM406_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010112296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVL77419.1"
                     /translation="MPVLALDHVNIRVAPELLETVLSFYLNVVGLREGFRPPFGSTGH
                     WLYAGAHAVIHLSVLRPNESPPRPGGTVDHIAFHCRDLEATCARLHALGQPYRRTEVP
                     HTRQHQLFLRDPAGNGVELNFDA"
     gene            16150..16530
                     /locus_tag="VM406_15505"
     CDS             16150..16530
                     /locus_tag="VM406_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HVL77420.1"
                     /translation="MHIFTYGSLMFSEVWQRVVPGSHASVPARLPGYRRSAIRGVSYP
                     GIAADDSAQVDGRLYLDVAAEDLAALDRFEGGEYARVAVSVVLEDGARFEAQAYVYLY
                     PDKLTGAVWDPGHFDCAHFLRFEA"
     gene            16584..17333
                     /gene="pgl"
                     /locus_tag="VM406_15510"
     CDS             16584..17333
                     /gene="pgl"
                     /locus_tag="VM406_15510"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HVL77421.1"
                     /translation="MLELTDIASEDAAAEALAGAVASDLQAALERKPRALLLVSGGRS
                     PLPFFAALRAQPLPWERIDVSLVDERAVAPDHPDSNAALVAQHLLTGFASAARFIPLV
                     HAGDPQAQRDPWQLAVHAAQRANANADLAAADAVVLGMGTDGHTASLFPDSPHWKVAS
                     ESRDRYVPLQPGAAPHARVGLSLHALAGQRTGYLWTGGAAKSATLARLTRLAGDAAQG
                     RPQHEVLRQAGPLAMLIADPGFTLRVFHFPE"
     gene            17605..>17804
                     /locus_tag="VM406_15515"
     CDS             17605..>17804
                     /locus_tag="VM406_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVL77422.1"
                     /translation="MSTIISSALGDVSDAKRFSLLIANVRDYAIYLLDSQGIVSSWNA
                     GAQRFKGYQAHEILGTHFSVFYT"
BASE COUNT         2907 a         5821 c         5954 g         3122 t
ORIGIN      
        1 ccccgcttcc ggccgtgacc cgcgcgttcg atatcggcac cgggaccggc gtgctcgcag
       61 cggtgctggc ccggcgcggc gtggaacagg tggtggcgac cgaccaggac ccgcgcgcgc
      121 tcgcctgcgc gcgcgagaac atcgcgcgcc tcgggctggg cgggcaggtg caggtgatgc
      181 aggccgatct gtttccgcct ggccgggcga tgctggcggt gtgcaatccg ccctggctgc
      241 cggcgcgtcc tagttcgccg atcgagtacg cggtctacga tcccgacagc cgcatgctgc
      301 gcggttttct gaacgggctc gccgaccatc tcgaaccggg tggcgagggc tggctgatcc
      361 tgtccgacat tgccgagcac ctggggctgc gctcgcgcgc cgaactgctt gcgatgattg
      421 acggcgccgg ccttacggtg ctcggccgcc tggacgcaag accgcgccac ccgaaagccg
      481 gcgacccgga cgacccgctg taccaggcgc gcaagaagga agtgacttcg ctctggcgcc
      541 tggcggcgaa atgagacccg ggagaaggcc gcgcccgcag tgcctggttg aacttatccc
      601 tcgaggttcg cccacggctc gcttgcgaag cgttccgcca gatagttgat cagggcctgt
      661 acgcgcgccg gccggttgcg acccggcggc gtcacgatgt gcaaggcgat gggttccact
      721 tgccaatcgt ccatcacggt ttccagcacc cccgactgca cgtcatgcca ggcgaggaat
      781 tccggctgca atgccagtcc caacccggcc cgcagggccg gcaccagcgc ttcggcattg
      841 ttggcctgca ggttcgaagg catggcctgg gcaaactcgc catgccgctt gtgatgaaac
      901 cgccagctcg taccgccgcg cgtataggtg tagagcaggg cgcgatgttt cgccaggtcg
      961 tgtggatgct tgggacggcc atgccgcgcg aaataggcag gcgccccgac cagcaacacg
     1021 cgcacgctgc aaaggcgccg ggccagaagg ctggaatccg tcatcctgga aatgcgcaac
     1081 gcgagatcga accggttagc taccaggtcg accagttcat cgttgaactg aatgtccagc
     1141 gccacgtcgg gatgcatcga catgaaatcg ggtagcagcg gcgagaggcg ttctatgccg
     1201 aacgacatcg gggctgcaac gcggatcagc ccgcgcaggc tgttcgattg ttcggcgatc
     1261 tccgcttcca ccgcttcgcc atcctcgagg atgcgcgtgg cccgttgcag tgcggcattg
     1321 ccgctcccgg tcagcgacag gcggcgcggc gtacgatgga gcagcacggt tttcatgcgc
     1381 gcctcgagcc gcgtgatggc cttcgacacc gtcgcttgcg aaacggaaag ttcggcggcg
     1441 gcccgggcga atgaaccggt ctcggcaact ttggcaaaga ttgcccatgc ttcgaggtct
     1501 ggtaattttt tcatcgtttt tcatggtagg cagattgccg gatgagcaca agccgtccgg
     1561 ctgacatgca acaaatggaa agcatgaaat tccattcttt ccattctcaa catgtcgaag
     1621 cgggcgcaca ctgcatacat cgccaccaga tgttttcagg agaacgccat gatcgaacgt
     1681 cgtcctttcg caagcctcgg cggagcccac cacggctggc tcgatgccaa gcaccacttc
     1741 tcctttgccg aataccacga tccggcccgg gtgcattggg gcgcattgcg ggtctggaac
     1801 gacgacacga tccagccccg taccggcttc ccgccgcatc cgcacgcgga catggagatc
     1861 atcacctacg tgcgcgaagg cgccatcacc caccaggaca acatgggcaa caagggccgc
     1921 acggtggccg gagatgtgca ggtgatgagc gccggcaccg gcattcgcca ttcggaatac
     1981 aacctggagc ccgacatcac gcgcatcttc cagatctgga tcgttccgga ccggcgcggc
     2041 gagccgccgt cctggggctc caagccgttt cccaagagcg atcgttccgg caggttctca
     2101 acgctggcca gtggcttaga aggtgacgag gacgcgctgc cgatccgcgc cgacgcgcgt
     2161 gtgctgggtg ccacgctcaa ggcgggggaa accgcggagt accgcttttc cggcaagggc
     2221 cgccacggct acctggtacc ggccatcggc accgtcgaga tcaacggcat tacgctggag
     2281 gcgcgcgatg gtgccgcgat caaggacgag gagatcgtcc gcgtcaccgc ccgcgaggat
     2341 gccgaactgg tcctggtcga cacagccgcc taagcatttc ctggtgcgag ggatgcctgc
     2401 cgcatccccc gcaccaggcc cgcgccgcgc tcgtcaggcc atggtccgga tccattctgt
     2461 acgcactcgc attgcacgcc accgtttcca accgttccat acctgaggga gaaacccgga
     2521 tgtccacgta cctcgaagtt ctgacgccgc aaaacagcca actcatcttc atcgaccagc
     2581 agccgcaaat ggccttcggc gtgcagtcga tcgatcgcca gctcttgaag aacaacgtcg
     2641 tggcgctggc caaggccgca aaggtgttca atatcccgac caccattacc accgtcgaga
     2701 ccgacggttt ctccggaaac acgtttcccg aactgctcgc cgtgttcccc gagaacaagc
     2761 tcctcgagcg cacctcgatg aattcctggg acgaccagaa cgtgcgcgat gcgctggccg
     2821 acggcgccgc acagggacgc aagaagatcg tcgtttcggg gctttggaca gaggtgtgca
     2881 atattacttt cgcactctcc tgcctcaggg ataccgacta cacgatttac atggtcgccg
     2941 acgcatccgg gggcacctcc gtcgatgcgc acaagtacgc gatggaccgc atggtgcagg
     3001 cgggcgtcgt gccggtcacc tggcagcagg tgctgctcga gtggcagcgc gactgggcgc
     3061 gcagggaaac ctacgacgct gtgctggcga tcgtgaagga gcattccggt gcctacggca
     3121 tgggagtcga ctatgcgtac acgatggttc acaaggcgcc cgagcgcgtt cggcacggcg
     3181 aacgcatcgg ccccaatccc gcgaagtaag gaaaccgacc atgccgcgtc ttcgcgcagt
     3241 ttcgtgggaa gaacgaaggt gagcaacgac gtggctgcca agggatctgc cgccagatcg
     3301 accgcgggcg gacccaccgc gcgcgcatcg cgatcggacg acagcgcatc tgacaaccgg
     3361 ccggatcgcc gccgagtccg gatacggcat taaccatggc agacggagca cagatcatgg
     3421 ccgattcctt ccccgatatc attttccatc acgcccggat cacgacgctg gatcgcggca
     3481 accccgtggc gagcgccgtg gcgatcaagg acggcaagtt cctggcggtg ggctccgacg
     3541 cgcagatcct ggccctggcc ggtgccggca cccggcttgt caacctcaag cacaggtgcg
     3601 tgctgcccgg cctgttcgac aaccacaccc acgtggtgcg cggcggcctc aactacaacc
     3661 tggagctgcg ctgggacggt gtgcgctcgc tggccgacgc gatggaaatg ctgcgccgcc
     3721 aggtggcgat caccccagcg ccgcagtggg tgcgcgtggt cggcggcttc accgagcacc
     3781 agttcgtcga aaggcgcctg ccgacgatcg cggaaatcaa cacggtggcg cccgataccc
     3841 ccgtcttcct gctgcacctg tacgaccgcg cgctgctcaa cggtgccgcg ctgcgcgcgg
     3901 tgggctacac caaggacacg cccgacccgc cgggcggcga gatcacgcgc gacgccgccg
     3961 gcaacccgac cggcctgttg cttgccaagc ccaacgccct gattctctac gccacgctgg
     4021 ccaaggggcc gaagctgccg ttcgactacc aggtcaattc gacccgtcac ttcatgcgcg
     4081 aactcaaccg cctgggggtg accggcgtga tcgacgccgg cggcggcttc cagaactacc
     4141 cggaagacta cgcggtgatc cagaagctgg ccgacgaggg ccagatgacg gtacggctgg
     4201 cctacaacct gtttacgcag aagcccaagg cggaaaagga cgacttcctc aagtggacct
     4261 cgtcggtccg gtacaagcag ggcaacgact actttcgcca caacggcgcc ggcgagatgc
     4321 tcgtgttctc ggccgccgat ttcgaggact tccgccaacc ccgccccgac atgccgccgc
     4381 agatggaagg cgagctggag gaagtggtgc gcatccttgc gcagaacaaa tggccgtggc
     4441 ggctgcatgc gacctacgat gaaaccattg cgcgcgcctt ggacgtgttc gagaaggtca
     4501 accgtgatat cccgctgcaa ggattgaact ggttcttcga ccacgccgaa accatctccg
     4561 acaagtcgat cgaccgcatc gcggcgctgg gcggcggcat cgccgtgcaa caccgcatgg
     4621 cctaccaggg tgaatacttc gtcgagcgct acggcgcccg ggcggccgag gccacaccgc
     4681 cggtggctcg cgtgctggaa aaaggggtga aggtttcggc cggcaccgat gccacgcgcg
     4741 tcgcctccta caacccctgg gtggcactgt cgtggatggt gaccggcaag accctcggcg
     4801 gcctgcgcct gtacccgcag cgcaactgcc tggaccgcga gacggcgctg cgcatgctga
     4861 ccgagaacgt ggcgtggttc tccaacgagg aaggccggcg cggccgcatc gagccgggcc
     4921 agttcgccga cctgatcgtg ccggccaagg actacttctc ggtccccgaa gacgagatct
     4981 ctttccttgc ctccgacctg accgtggtcg gcggccgcat cgtctacggc gatggcgatt
     5041 tcgccagcct cgacgacaac ccgctgccgc cggcgatgcc ggactggtcg cccacgcgca
     5101 tcttcaaggg ctatggagcc tggggcgagc cggagggcgc gggccggcac tcgctgcacc
     5161 cggcacgcta tcaggcgatc gcctcatgcg gctgcgccag tggctgcggc ctgcacggcc
     5221 acgaccacgc tcgtgcctgg gacgccaagc tgccggtgtc ggatatgcgg ggcttcttcg
     5281 gcgccctggg ctgctcgtgc tgggcggtgt gaggtcgacg cataaagaac gggagacacc
     5341 atgatgcaag caccgcaaac ggtgtccgca ccttcgagca gctttgctcc attgcggcaa
     5401 aggctgttcc tcgtgctgtg gctggccacg atcatcggca acaccggcag ctttatccgg
     5461 gacgtggcca gcgcatggct gatgaccgac ctgtcgcctt ccccgacggc cgtggcgctg
     5521 gtgcaggcag cggccacact gccggtcttc ctgctggcga tccccgccgg cgtgctggcc
     5581 gacatcctcg accggcgcaa attcctcatc gctatccagt tgctgctggc cagcgtcagc
     5641 atcagtctga tgctgctgtc ggccttcggc ctgcaatccg tgaccacgct ggtcgcgttc
     5701 acattcctcg gcggcgtcgg cgcggcgctg atggcgccga cctggcaggc catcgtgccc
     5761 gaactcgtcg agaagaaaga tctcaagagc gcggtcgggc tcaattcgct cggtgtgaac
     5821 atctcccggg ccatcgggcc cgcaatgggc ggattgatcc tggccagcat gggggcggca
     5881 ttcacctatg gcgtcgatgt cctcagttac gtatttgtcg tcacggtcct gctgtggtgg
     5941 cggcggccaa gcgccgcgca ggatgtgctc gccgaacgtt tcgcaggcgc ctttcgcgcc
     6001 gggttgcgtt atgtgcgcgc cagccgcgcg ctccacgtgg tactggcgcg cgccttcttt
     6061 ttcttcgccc tggccagttc ggtctgggcg ctgctgccgc tggtcgcgcg ccagctgctc
     6121 ggtggcggcg ccagtttcta cggcatcctg ctcggcgcgg tcggcatggg ggcgatcgtc
     6181 ggcgccatca tcatgccgac gctgcgccaa cgcctgagcg ccgatggact gctgctgctg
     6241 gccgcattgg tcaccgcgct ggtcatggcc ttcctgtcct tcgcaccgcc gcagtgggcg
     6301 gcagtggtca gcctgttgat gctcggtgct gcctggatca ccgcgctgac caccctgaac
     6361 ggcgtggcac aggccatact gccgaactgg gttcgcggcc gctcgctggc ggtctacctg
     6421 accgtcttca acggcgccat ggccgcaggc agcctggtct ggggaacggt agccgagcgc
     6481 atcggcgtgc cgttcacgct attggttgcg gctgccggtc tggtcgcggt tggactggtc
     6541 gcccatcggt tgaagctgcc aaagggcgag cttgacctgg tgccgtccaa ccactggccc
     6601 gagcccctga ccgctgcgcc ggtagagcat gatcgcggcc cggtgctgat cctgatcgaa
     6661 taccggattg cggcggagga tcgaggcgct ttcctgaaag cgctcttcga attgtcagcc
     6721 gaacgccggc gcgatggcgc ctacgcctgg ggcgtcagcg aggatgcagc cgatcctggc
     6781 ctgatgctcg aatggttcga ggtcgagtcc tgggcggaac acttgcgcca gcatcatcgg
     6841 gtctccaagg ccgacgccga cgtccaggcg gaagtcctga gtttccatcg cggcgagggc
     6901 cggcccgtgg tgcggcactt cctggcatgt acccgcccct aggacgtgca ggcgtgcgca
     6961 ggcttccctc gtttgatcac gcttaaagct gcgttttggc gcggcgtttt cctacatgcc
     7021 cgaacggcgg attccgatat gcaacgggat gaggcgtgga tatagtcctt ccaacgcaaa
     7081 catcaaccgg agtcagccat gagtgacgaa gccgatctcg ccgccgagac gattgaactg
     7141 agcaggatgg ccgccatcga agcatgccgg cgtcaaccgg cattgccggc caagggttct
     7201 tgctggtttt gcgatgaagc cctgccgccg aaccagaagt tctgcgatgc cgattgcgca
     7261 tccgactacg aactggaaca ggccgcctgg gcgcgcgccg gacgcggcaa gccgagcgat
     7321 ctctacgcgg attagtcgtt gaagcattga cgggctgcat cgccggtcga tgcggcccgt
     7381 ttatactacg gcggttccgc ccgtgacccg agccgggtgg cggcttcgcg atccctcatg
     7441 tcgagcactt ccaacgcttt tctggtcggc gcgctgttct gcgtgctgat gcttctggtt
     7501 ctcagttccc tgtttcgtag cggcctcccc ggcatccgcg agctttttct cgccaacttg
     7561 ctgggcggca tcgcgctggt gctgtacgcg ttcgggcgcg aggtgccgcc gctggtcggc
     7621 tatgaagtgg ccaacggcct ctacgcggca tccgcggcag ctgtgctggc cggctaccgc
     7681 cgcttcctcg gcgagcgcgt gtcgtggccg ctgctcatcg ccggcgtggg actggtggtc
     7741 gcggccatcg cgctgttcca ttaccggatc gactcgttcg cgctgcgcac cacgacgatt
     7801 gcggtgttcc agtcggcgct gtgcattgcc atcatcgtca gcatctttgc cgcgcgtcac
     7861 cgctggcgtt cccgctatcc gtactacttc gccgccgcga tggcgggact gatcgccgtc
     7921 gcccacatca cgagaagcct ggtccacctg cagcgcacgg atgagatcac ttcgctgctg
     7981 cagccttccg gctggaacct gttcttcgtg tccgccggca ccttcgtgct gccggtgctg
     8041 accatcgccc ttgtgatgat ggtccacgac gcgatgatgg cgcgcgccga gcacgcggcc
     8101 aatcgcgatt tcctgaccgg cgcctggtcg cgccgcgcgt tcttcgagct gggcgagcgc
     8161 gaactctcgc gcgggcgccg ccatgcacgc cggctcagcc tgcttctgct cgacatcgac
     8221 cacttcaagg cgatcaacga caactgggga catgcggcgg gcgaccaggt gctcatcgac
     8281 acggtggcgc gcgccggcgc ggtgctgcgc aacggcgatt tcttcgcccg catcggcggc
     8341 gaggagttcg gcgtgctgct gccggagacc gaaggcgtgg aggcggagcg ggtcgccgaa
     8401 cgcctgcgcg cggcgctcga tcacacgctg atcgtgcacc agggccggcg caagaccgca
     8461 tcggtgagct gcacggtcag catcggcttc gcggtgcagg ggcgcggcga aaccctgcac
     8521 gacattctgc ggcgcgccga cgcggcgctg tacgaggcca agcaggcggg gcgcaaccgg
     8581 gtcgtggcgg cggcggacgg cggacgttga aggtcggccg cgccgaaggt tttcgatgag
     8641 gccctcggca tggcgctgcg agcgatctct tgtccttaca actgccgcac gcacagccgg
     8701 aaatccgggt cttcctcgta gcgcgtgatc tcgaactcca ggctgcgcat cagcttgagc
     8761 atcggttcat tggttgtcag taccagcccc tcgatgcgcg tgaggccctt gcgccgtgcg
     8821 aactccatga tcgagagcat catgcgcgag ccgatgccct ggccgcagaa ctcgtcggcc
     8881 actgccagcg agaattcgca ggacaccgaa tccggattgg tcacgtagcg cgacaccgca
     8941 atgatgcgct cgctgtcgac cagcgcgccg tcgcgtcccg gcttgcgttc gcgcagcacg
     9001 gccacaaggg ccatctcgcg gtcgtagtcg atcagcgtga agcgcgcgag catcttcaca
     9061 gacagctcgc gcatcgtcga gacgaagcgg ttgtagcggc tctcgggcga catgcggcgc
     9121 acgaagtcct gcagcatctc ggcgtcgtcc ggatggatgg cgcgcacggt gtagcggccg
     9181 ccgccgcgca tcggccaggt ctgctcgaaa tcggccgggt agggcaggat ggccaggtga
     9241 gggtaggact ccggcgtgcc gggcgcgggc ccgaccacga tgcgcgcgtc gaccgcgatc
     9301 gcaccgttgt ggtcgacgat gaccggattg atgtccatcg ccaccagctg cggcagcgcg
     9361 cacaccattt ccgaaacgcg cagcaggata tgctcgagcg cctcgcgctg cgccggtggc
     9421 gcgccgcgcc atgggccgag cgtgtcggcc gcgcgtgcgc gctcgatcag ccgctgggcg
     9481 aggaactggt tcagcggcgg cagctcgatc gcgcggtcgc cgatcagctc gaccatggtg
     9541 ccgccggcgc cgaaggtgat caccgggccg aacggttcgt cgctggccac gccgatgtac
     9601 agctcgcgcc cctgcggctt gccggacatg ttctggatcg tgatgccgtt gagcttagca
     9661 tccggacagc ggcgcgcgac agtgtccatc atgtcctgcc aggcgtcacg cacggcggcg
     9721 gcattgtgca ggttcaatgc cacgccgccg atgtcggact tgtgcgtgat gtcgggcgaa
     9781 tcgatcttca acgccaccgg atagccgagc tggttggcga tcaggatcgc ctcgccggcc
     9841 gagcgcgtca ggatggtctt cgtgatcggg atgtggaagg ccgacagcaa ggccttggac
     9901 tccatctcgg tcagcgtctt gcgccgttcg gccagcacgc tttcgaccag gatgcgcgcg
     9961 ccttcgacat cgggcttggc cagcggcgac atcggcggcg gcgtctggta cagcagctgc
    10021 tggttctggt ggaacgaggc gatgttgttg aacgcgtcga ccgccgcttc cggcgtgcgg
    10081 aaggtcggaa tcgccgcgcg cgcgaggatc ttgcgcggct cggcggcgcg ctcgtcgccc
    10141 atccagcagg tgatcagcgg cttgccgatc ggctgcagct ccgccagcgc tttcgcgatc
    10201 gcttcggtgt ccagatgcgg cttgggcgac tggatcacca gcatgccgtc gaccgcgttg
    10261 ctggccgcgc aggcggtcac cgcggcgcgg tagtgggcgg cgtcggcttc ctcgccgagg
    10321 tcgatcagcg actccagctg ggccagcggc gacaactggg gcgcgagcct ggcggcttcg
    10381 gccgcgggca gggtggccag ctcgagaccg aggtcgttgg cccagtcggc cgccagcacg
    10441 ccgggcccgc cgccattcga gatcacggcc aggcgcttgc ccacgggcag gtagcgcgaa
    10501 gccaggcatt tcgccgccga gaacagctgc acgaaggaac gtacccgcac cgcaccggcg
    10561 cgctgcagcg cggcgtcgaa cacgtcgtcg ctgccgacga tggtgccgga gtgggtcaat
    10621 gcggcgctcg atccggccgg cttgcgcccg gccttgagca cgatgaccgg cttgacgttg
    10681 gccgcattgc gcagcgcgct catgaaacgg cgcgcgtcgc gtatgccttc caggtagacc
    10741 aggatgcttt gggtggcgcg gtcggcggcg aagtattcga gcacctgcca caggtccagc
    10801 gcggtgtcgc ggccgagcga gacgacgccg gagaaaccta cgcggttctt gactgcccaa
    10861 tccaggatcg cggtggtcag tgcgccggac tgcgagatca gccccagcgg tccctgctgg
    10921 gccagcgggc ccagcgtgct cgcattaagc gaaaggcgcg gggtctgaaa gccggaactg
    10981 ttggggccga gcaagtggat gccatgacgg cgtgcaatcg cgtagagctg aacggcctgc
    11041 gctgcggaaa cgccgctgga aatgatcagg accgtcttgc actggatgcg cgcggccact
    11101 tccagcgcag cctgcagctg atcgttgggc agcgcaatga tgcccaggtc cgcgcgggaa
    11161 tgagcgagat cgctgagcgt gccacgcatg ccgatgtcga ggaaggtgac cgggccgcca
    11221 tagccgccgt cgctgagctg cgcgatgatc tggcgcccat gggcaggatg gttcccggac
    11281 tgattcgggt cgccggcgaa cacggcgatc gagcgggggg cgaacagcgg ggtcaggtaa
    11341 tggttttcca tgcggaatcc gaagcgtgcg gcgcgtgtgc ggggctgcgc ggcccctgaa
    11401 acagtccata acagactcta ggcatcgacg ccgatcagaa ccttcacgtg cgccgccgcg
    11461 ctgcgtgcca gcgcatggag ctcgtagccg ccttccaggc atgaaaccac acggccgccg
    11521 gcatggcgat gagcgatgtc catgattgtg cgcgtgaccc atgcgtaatc ggcctcgacc
    11581 agcgcgagat tgccgagatc gtcgtcgcga tgcgcatcaa agccggccga cacgaagatc
    11641 atctgagggc ggaacgcctc cagcgcaggc agccagtgcg cttggactgc cttgcggaag
    11701 gcctcgcttc cggagcgggc ggcgagcggg atgttgacca tgttcggacc catcggctcc
    11761 gtgccggaga acgggtacag atcgcgttcg aaggtcgaac acatcagcgt gcgcggatcg
    11821 tcgcagagga tgtcctcgct gccgttgccg tgatggacat cgaaatccac cagggccacg
    11881 cgctccaggt tgtgcacgtc cagcgcgtgc cgcatgccga ccacgacatt gttgaagaag
    11941 cagaagcccg aggcgaagcc gcgtccggca tggtggccgg ggggacgcac attgcagaag
    12001 gcaaccggag ctttcccttc cagcaccagg tcggtggcga gaaccgccgc cccggccgcg
    12061 cgcaacgcgg cgtcgtaggt gtgggggttc atccaggtat ccgggtcgat cgccgcatag
    12121 tcttccggcg gcaccctggc cgcgatctca tgcacgtgca gcagcgcatg cgcgcgtccc
    12181 agttgcgcct cagtcgcacg cggcgcatcg taggcctgca tggcgtcgag caggcccttg
    12241 gccaggaaat ggtcgtggat cgcgtccaga cgctgcggac attcagggtg cagcgcaccc
    12301 atttcgtgct tgtggcaatc ggtgtgggaa atataggcgg gcagcatgcg gcgcaagacg
    12361 gatgggcgat agaacggctc agcccattgt acacagcggc atcgcgcagg gaggtcggcg
    12421 cttgccgcgc ggcggaggcg gtgcgtcgat tcttgactca gatcaaatgg cggggcgggt
    12481 cgcgcgacgc tgttgcagcc atgcgcgaac ggcgacggtt ctgcgcaatc tccatttctg
    12541 ttttcggccg ccacgcagaa tgaaatggtt cgaatgcggt ggagccggcg cggatgtttc
    12601 gccggaccgg cccccaacgt gacaggaagg cagatgtctc cacgatttgc ggcctgcagc
    12661 ggccagcgcc actttgtacc gcgagcccgc acgggtgacg gcaagcgggc tgcggccatt
    12721 taccgcctgc gcgctgcgcc tatagtcccg ccaatcgcat gtcacgcaga cgcgagccgg
    12781 ctcgcaatgc gcgcgccatc actgcgcgtg ctggtgaact gttcgggcta ggccatgatg
    12841 tttactacac gggatccgat atccctgaca cgcaccgtcc tgcttggcct gttcctggtc
    12901 gggctgttca tcctgtccta ccaggtgctc agactgttca tcgtgcctgt ggcctgggcg
    12961 ctgatcctcg cgtatgtcac ctggcccgtc tatgtgcacg tgcgcgcggc catcggcggc
    13021 agtccgacag ccagcgcggt ggtgatgacg ctgctgttga ccaccgcctt cgtgctcccg
    13081 ctcttgtggc tgatcggcct gctgcagaac gaattcgtgc tcgccttcaa ggccgtcagc
    13141 acttatctca acgacgggcc gccgcttctg cccgcattca tacgcgacat cccatggctg
    13201 ggcgagttcc tgcagcagct gatcgagcgc atggcgatcg atcccgaggc gatgcgcaag
    13261 gaaatgctgc agtgggcgca gggttgggtc ggccacgtga ccgacctgat cggcggcgtg
    13321 ggacgcaatg cggcgaagtt cgcgatggtg gtcgtcaccg ttttgttcgt ctaccgcgac
    13381 ggcgaaagtc tgctcaagca ggtgcgcaag gtttcgcgcc gcttcctcgg tgcgcgcgcg
    13441 aacgcctatc ttgccgcgat cggcgccacc accacggccg tgctgtatgg gctgctcctg
    13501 accgccatcg cccagggcac gctggcgggc atcggctact gggcggccgg cattcgggcg
    13561 ccggcactgt tcggtgcgct gacggcgctg atcgcgctgc tgccgttcgg tgcgccgttc
    13621 gtctggggca cgctgtctgt ctggctgctg gtgcagggcg agacctgggc cggcatcggc
    13681 cttttgctgt ggggcatgct ggtggtcagc tgggtcgaca acctgatccg gccgctggtc
    13741 atcagcagtg cgaccaagat ccctttcctg ctggtgatgt ttgcggtgct cggcggcctg
    13801 gccgcattcg ggctggtcgg catgttcctc gggccgatcg tgatcgccgt gctgctggcg
    13861 gtctggcgcg aatgggtcga tgccgagccg ccggcgccgc cgccgccgcc ggaggaggac
    13921 acgccggtct gagcggccga tctgaatacc ccgttatgcc agtcgcggcc gccccaaggg
    13981 cagccaggcc agcgccctgc gcgcgctggt gcgcgcgtcg cccagcacca ggcgcgcacc
    14041 gtcgtcggtc agcggcaccg ccaccgcgag gccatggcga tcacaggtga agtcgtcgcc
    14101 ggtcgccagc accaggtcgc gcgcatcatg atcctgggtc acccagacgg cgcctgcgac
    14161 cacgcggatc gtcgttccct tcgcgttctt caggcgcagc atctggccgc gcgtcagttc
    14221 gagcactgcg tcctcgcgcg gcagcggcag taccgccccg cgcatgccga gaatctgcat
    14281 ggttcctttc atgatccgct ccttgaggtg agggacgctt gtggcgccct tgcacgtcag
    14341 tattggccgg tgcgggctgg tgcacaatcc ctggataagg aataagattg ttcgcaaaag
    14401 agaacaatgg gagcgagtat ggatcgactg ccggaaatga tgaccttcgc caaggtcgtc
    14461 gaaacccaca gcttttccgc cgccgcgctt gccctcggca cgtcgaagtc ggtcgtcagc
    14521 aaacaggtca gcgcgctcga ggccgcgctc ggcgtacgcc tgctcaaccg caccacgcga
    14581 cgcatgagcc tgaccgaggt cggcgccgcg tattacgagc actgcgcgcg catcgcccgt
    14641 gagatcgagg cggccgcgca aaccgtctcg cagctgcagg ccgagccgcg cggcgtgttg
    14701 cggctgacgt cgccggtgat cttcgcctcg ctgcacctgg cgccggcgtt gcacagcttc
    14761 atgcagcgtc atgaacacgt cgaagtggaa ctgaacgcgg tcgatcgggt ggtggacatc
    14821 atcgaggaag gctacgacct cgcgatccgc atcaccggca atccgccgca gaacatggtg
    14881 gcgcgcaaga tttccgacgt gcgctgggcc acctgcgcgg cgccgtcata catcgcgcgc
    14941 cacggcgccc ccgccatccc gcaggacctg ctgcggcatg cctgcctcac ttacgagggc
    15001 attccggcgc tgcgcggcgg cgactggcgc taccgggtgg cgggccgcga gctgagcgtg
    15061 cgcgtcggcg gtcgggtacg agtgaacaat tcggaagtgc tgctgcagat ggcgctggac
    15121 ggcgccggca tcgtgctgtt tcccacctac atcgtcggcc cgcacctgca gagcggccgg
    15181 ctggtcgaag tgctcaggga cagcgtcgcc aatcctgaca tggcactggt cgccacctac
    15241 ccgccgaacc gctacatgca gccgaaggtg cgcgcgttca tcgaccacct gcttgcgcat
    15301 ttcgggccgc agccgtattg ggaccggtac tgaatcaggc gtcgaagttc agctccacgc
    15361 cgttgccggc cggatcgcgc aggaacagct gatgctggcg ggtgtgcggc acctccgtgc
    15421 gccggtaggg ttgaccgagc gcatgcaggc gcgcgcaggt cgcctccagg tcccggcaat
    15481 ggaaggcgat gtgatcgacg gtgccgcccg ggcggggagg cgattcgttc gggcgcagca
    15541 cggagaggtg gatgacagcg tgcgcgccag cgtaaagcca gtggccggta ctgccaaacg
    15601 gcggtcgaaa gccttccctg aggccgacca cattcagata gaagctcagg accgtttcaa
    15661 gcagttccgg tgcgacgcgg atattgacgt ggtccagtgc aaggacgggc atggcggttc
    15721 gaggcggatg gagcgactga ttgtatcgct tgaaggcgca aacgtcgcgc ggtccggcca
    15781 tgctgggttg ccttcggcgc gaaaatcaag acggtctata tccgcctccg ccgttacctg
    15841 atgtttcgcg aatccagtag cacgctgaac gcggtgcgca tccgcccgtg ctggcacgcc
    15901 tgcacgcaaa gggaacgggc tgcaaggaag attcggcgag cagcccgtac cttccctgca
    15961 cacgagcatc gggccctggg ccgggccgtc gtcaatatcg gggttgaagg ggggctgctg
    16021 ctccatttga ctgtgcggca atgaaaagtt cgctgactgc ttcagcgctt atctcgcgaa
    16081 ccgggacgtg tcttgcgaaa atggcaggca tcttgctatc gcgagggtct gcgcacaggc
    16141 tcggaatcca tgcacatctt tacctacggc tccctgatgt tttccgaggt ctggcagcgc
    16201 gtcgtgcccg gcagccatgc gagcgtaccc gcccggcttc ccggttatcg gcgttcggcg
    16261 atccgcggag tcagctatcc gggcatcgcg gccgatgaca gcgcgcaggt ggatggccgt
    16321 ctgtatctgg acgttgccgc agaggatctg gcggcgctcg accgtttcga gggcggcgag
    16381 tacgcgcgtg ttgcagtgtc ggtcgtgctc gaggacggcg ctcggttcga ggcgcaggcc
    16441 tacgtctacc tgtatcctga caagctgacc ggcgcggtct gggatccggg ccatttcgac
    16501 tgcgcacact tcctgcgctt cgaggcatag cggccgcctc gacgccgtat ctgccgccgg
    16561 ttcggccggc atgaggagca ttgatgttgg aattgacgga tattgcgagc gaagacgccg
    16621 cggcggaggc gctggccggg gcggtcgcgt cagacctgca ggccgcattg gagcgcaagc
    16681 cgcgcgcgtt gctgctcgtg tccggcggtc gcagcccgct gcctttcttt gccgcgctgc
    16741 gcgcgcagcc gctgccctgg gagcgcatcg atgtctcgct ggtcgacgag cgcgcggtcg
    16801 cgccggacca tcccgactcc aacgccgctc tggttgcgca acacctgctg acagggttcg
    16861 cgtccgcggc ccgcttcatt ccgctggtcc acgccggcga tccccaggcg caacgcgacc
    16921 catggcagct tgccgtgcac gcggcgcagc gcgccaacgc gaatgccgac ctcgccgcag
    16981 cggatgcggt cgtcctcggc atgggcaccg acgggcatac ggcctcgctg ttccccgact
    17041 cgccgcactg gaaggtggcg agcgagagcc gggaccgcta tgtgccgctg cagccggggg
    17101 cagccccgca tgcgcgggta gggctgtcgc tgcatgcgct ggcggggcag cggacgggtt
    17161 acctctggac gggcggcgcg gccaagtccg cgacgctcgc gcggttgacg cgactcgcgg
    17221 gagacgccgc tcagggtcga ccgcagcatg aggtgctgcg ccaggccggc ccgctcgcga
    17281 tgctgatcgc agatcccggc ttcacgctgc gcgtgtttca cttccccgaa tgacggacga
    17341 tcgaaccgtt cagaggcatg gcgactgcag ggggttattt cccgtgatca agctttgcgc
    17401 gagggcaaac ttttgagaat cggcgcggtt ccgtgaagcc gcgccgcgca tactagacca
    17461 agtgcgcgaa ccgagcgggc atgcagcagc gcatgtccgc ccggcgtatt cctgctatcg
    17521 ttttaccttc gagacgcgcg ccgcgccgct acggagcatt gtccggggcg gtgtttcggc
    17581 cgttccgtca aggccaatcc ctacatgagc acgatcatct catccgcgct tggagacgtc
    17641 agtgacgcca agcgcttcag cctcctgatt gccaacgtca gggactacgc gatctacctg
    17701 ctggattccc aggggatcgt cagcagctgg aatgccggtg cgcagcgctt caagggctat
    17761 caggcgcacg agattctcgg cacccatttt tcggtcttct atac
//
LOCUS       DATLGF010000498         5643 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_107091, whole genome shotgun sequence.
ACCESSION   DATLGF010000498 DATLGF010000000
VERSION     DATLGF010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107091"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..745)
                     /locus_tag="VM406_15520"
     CDS             complement(89..745)
                     /locus_tag="VM406_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVL77423.1"
                     /translation="MTSKEQEPDVETGVVGRNPARTRQAIIDAATEEFATLGLGGARI
                     DSIAARAGLNKRLLYYYFGNKDALFLAVLERAYENIRNAERALQLDELDPVEAIRQLV
                     SFTWRYYIAHPEFLTLLNSENLHQAAHLKTSERIQEMNSPLIETLDRILEKGRASGLF
                     RAGVDPLQLYISIAGLSFFYLSNRHTLSTVFGRDLRHPKAEAERLSHMTDLVLGYLLR
                     "
     gene            complement(842..1708)
                     /locus_tag="VM406_15525"
     CDS             complement(842..1708)
                     /locus_tag="VM406_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HVL77424.1"
                     /translation="MRDFTPDHRWLSINTATVRAQWKLDRIIEECAQRGIRAISPWRD
                     QVAEIGLDKISAMVRDAGLELSGYCRGGMFPASTQQGRQRALDDNRRAVDEARELNAA
                     CLVLVVGGLPGGLAGKPEHKDIAAARNDVRDGIAATLDYARTVGMPLAIEPLHPMYAG
                     DRACVNTMEQALDLCDLLDPQRSGGLGVALDVYHVWWDPKLEDQIRRAGRERLLAFHV
                     CDWLLPTRDLLNDRGMMGDGVIDIPKIRGWVEAQGFAGYSEVEIFSTENWWKRDGGEV
                     LDTCIARHRSVV"
     gene            complement(1960..3147)
                     /locus_tag="VM406_15530"
     CDS             complement(1960..3147)
                     /locus_tag="VM406_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HVL77425.1"
                     /translation="MSLTLRLPAADGSLQPYTLSGRAAIAPPAAPAFNRIAYSAAHVV
                     ADPTAAVDPWLAAAIDWDATIAYRRRLWSMGLGVAEAMDTAQRGMGLDWSTSLELIRR
                     SLDAARDFPNAIVASGCGTDHLAPEAAQSVDDVIRAYEQQMEAIEALGGRLILMASRA
                     LARVARGPADYERVYGHLLQQAKQPVILHWLGDMFDPALAGYWGTKDLDAAMDVALGI
                     IEANAAKVDGIKISLLDKDKEIAMRRRLPAGVRMYTGDDFNYAELIAGDGEGKQPNHR
                     QSDALLGIFDAIAPAASAALSALAAGNEARFHEILAPTVPLSRHIFRAPTRFYKTGVV
                     FMAWINGHQDHFVMVGGQQSTRSLPHFAELLRLADAAGLIEQPELAAWRMRSLLAMHG
                     IEG"
     gene            complement(3155..4318)
                     /locus_tag="VM406_15535"
     CDS             complement(3155..4318)
                     /locus_tag="VM406_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HVL77426.1"
                     /translation="MSTQRLGLIMHGVTGRMGMNQHLIRSIVAIRNQGGVTLSDGSKV
                     MPDPILIGRNAEKIEALARAHGISRWGTNLDEALANKGDTVFFDAGTTQMRPTLLAQA
                     IRAGKHVYCEKPIATNLNEAVEICRLAQKSGLKHGAVQDKLFLPGLRKLDMLRRSGFF
                     GRMLAVRIEFGYWVFEGDLQPIQRPSWNYRKEDGGGMILDMMCHWRYVLDNLFGEVQS
                     VSCLGATHIPKRWDESGRPYEATADDAAYATLELKGHNGEHVIAQVNSSWATRVKRDD
                     LVTFHVDGTHGSAQATLTQCFSQSRVNTPRPVWNPDQKQTMNFPAQWQEVPDTQVYDN
                     GFKVQWEDFIRHVVEDAPYRYTLPEGAKGVQLVEDALQSWQERRWVDVKPLPL"
     gene            complement(4352..5320)
                     /locus_tag="VM406_15540"
     CDS             complement(4352..5320)
                     /locus_tag="VM406_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVL77427.1"
                     /translation="MKSVRTLGLGLIVGLASFSAVAQNAYPSKPIRVIVPFTAGSTTD
                     LVARVVTEKLSTSMGQPVIVENRGGAGGTIGAAQVAQAAPDGYTVLIHSASHTVSPST
                     YSKLTYDAIGDFAGVTPLATLPNVLVVAPSKNMRSLKDLLDHAKANPGKMNFASAGQG
                     SATHLNAEKFRTQAKIDAVHIPFKGSPEAVTEVMTGRVDYYFSPVAPVLPFLSDGRLV
                     PLAVGSPNRAALLPNVPTTSEAGVPGSEFNFWIGMMVPAKTPRDIVSKLNAEVKKALA
                     SPDVQERLAKLGAEGWHLSPEQWDQYLKDDVRTNAQIVKAAGIEPQ"
BASE COUNT          868 a         1784 c         1933 g         1058 t
ORIGIN      
        1 ggaataaacg ggaggcaagt gattcaggca gcgtgccacg cctggcccga tcgcctggcc
       61 cgatcgcctg gccgtgcgca ctggccgctc agcgcagcag gtagccgagc acgagatcgg
      121 tcatgtgcga caggcgctcg gcctcggcct ttggatggcg caggtcgcgg ccgaacacgg
      181 tggacagcgt gtgacggttg gacaggtaga aaaacgacag cccggcgatc gagatgtaga
      241 gctgcagcgg gtcgacgccg gcgcggaaca atccgctcgc cctgcccttt tcgaggatgc
      301 ggtccagtgt ttcgatcagc ggcgagttca tctcctggat gcgctcggac gtcttcaggt
      361 gcgcggcctg gtgcaggttt tcgctgttga gcagggtaag gaattcaggg tgggcaatgt
      421 agtagcgcca ggtgaacgac accagttggc ggatcgcttc aaccgggtcg agttcatcga
      481 gctgcagcgc gcgctcggca ttgcgaatgt tttcgtaggc gcgctcgagc acggccagga
      541 acagcgcgtc cttgttgccg aagtagtagt agagcaggcg cttgttcagg ccggcgcgcg
      601 cggcgatcga atcgatgcgg gcgccgccca ggccgagggt cgcgaactct tcggtggccg
      661 cgtcgatgat ggcctggcgc gtgcgggcag ggttgcggcc gaccacaccg gtttcgacat
      721 cgggttcctg ttccttgctg gtcatgcgca tggcctggat tgcgggggaa ggtcggatgt
      781 taatggaaat cggggcgggt gcaaggcagg aaagtcggtt ttcgggcgtt gatgcaggaa
      841 cctagaccac gctgcggtgg cgcgcgatgc aggtgtcgag cacttcgccg ccgtcgcgct
      901 tccaccagtt ctccgtcgaa aagatttcca cttcgctgta gccggcaaag ccctgcgcct
      961 cgacccagcc gcggatcttc gggatgtcga tcacgccgtc gcccatcatg ccgcggtcgt
     1021 tgagcaggtc gcgcgtgggc aaaagccagt cgcagacatg gaaggcaagc aggcgttcgc
     1081 gaccggcgcg gcggatctgg tcttcgagtt tcggatccca ccagacgtgg tagacgtcga
     1141 gcgccacgcc gaggccgccg ctgcgctgcg ggtcgagcag atcgcacaga tcgagcgcct
     1201 gctccatcgt gtttacgcag gcgcggtcgc ccgcatacat cgggtgcagc ggctcgatcg
     1261 ccagcggcat cccgaccgtg cgcgcatagt cgagggtcgc ggcgatgccg tcgcgcacat
     1321 cgttgcgggc ggcggcgatg tccttgtgtt cgggcttgcc ggccaagccg ccggggaggc
     1381 cgccgacgac cagcaccaag caggccgcgt tcagctctcg cgcctcgtcg accgcgcggc
     1441 ggttgtcgtc gagcgcgcgc tggcggcctt gctgcgtgga tgcagggaac atgccgccgc
     1501 gacagtagcc ggacagctcc agcccggcgt cgcgcaccat cgcgctgatc ttgtccaggc
     1561 cgatctcggc gacctggtcg cgccacggcg agatcgcacg gatgccacgc tgcgcgcatt
     1621 cttcgatgat gcggtccagc ttccactggg cgcgcacggt ggcggtgttg atcgacagcc
     1681 agcggtggtc gggggtgaag tctctcatgg gtgttttggt tgggtgatgg gggtgccggg
     1741 gcgtcgcaac ttggcatttc tgaatgaatc gctgcgacgg ctgcatcgtc cactgcggac
     1801 agccccttcc cccctttcag ggggaaggtt gggtaggggg tgcggagctc gaatcggcgt
     1861 ctgccttaat gcgggctccg cacccccacc ctacgccggg cgcccctagc cctctccctg
     1921 aaagggggag ggaacggtcc gtagggtgct ccgatccggt cagccctcga tgccatgcat
     1981 cgcaagcagg ctgcgcatcc gccacgccgc cagttccggc tgttcgatca agcctgccgc
     2041 atcggccagc cgcagcagtt cggcgaagtg cggcagcgag cgcgtgctct gctggccgcc
     2101 gaccatcacg aaatggtcct ggtggccgtt gatccatgcc atgaacacca cgccggtctt
     2161 gtagaagcgg gtcggcgcgc ggaagatgtg gcgcgacagc ggcacggtcg gcgcgaggat
     2221 ctcgtggaag cgcgcctcgt tgcccgctgc gagcgccgac agtgccgcac tggcagcggg
     2281 cgcgatcgca tcgaagatgc cgagcagcgc gtcgctctgg cgatggttcg gctgcttgcc
     2341 ctcgccgtct cctgcaatca gctcggcata gttgaaatcg tcgccggtgt acatgcgcac
     2401 gccggccgga agacggcggc gcatcgcgat ttccttgtcc ttgtcgagca gggagatctt
     2461 gatgccgtcc accttggccg cgttcgcttc gatgatgccc agcgcgacat ccattgccgc
     2521 atccaggtcc ttcgtgcccc agtagccggc gagcgccgga tcgaacatgt cgccgagcca
     2581 gtgcaggatc accggctgct tcgcctgttg cagcagatgg ccgtacacgc gctcgtagtc
     2641 ggccggtccc cgggccacgc gggcaagcgc gcggctggcc atcaggatca gccggccgcc
     2701 gagcgcttcg atcgcttcca tctgctgttc ataggcgcgg atcacgtcgt ccacgctttg
     2761 cgccgcttcc ggcgcgaggt ggtcggtccc gcagcccgac gcaacgatgg cgttcgggaa
     2821 gtcgcgggcg gcgtcgagcg agcggcggat cagttccagc gatgtgctcc agtccaggcc
     2881 catgccgcgc tgggccgtgt ccatcgcctc ggccacgccg aggcccatgc tccacaggcg
     2941 gcgtcggtag gcgatggtcg cgtcccagtc aatcgccgcg gcgagccagg ggtcgacggc
     3001 cgcggtcggg tcggcgacca cgtgcgcagc tgaatacgca atgcggttga acgcgggcgc
     3061 ggccggcggc gcgatcgccg cgcggccgga cagcgtgtag ggttgcaggc tgccatcggc
     3121 agcgggcagg cgcagtgtca ggctcatggc gacctcagag cggcagcggt ttgacgtcga
     3181 cccagcggcg ctcctgccaa ctctgcagcg cgtcctcgac cagctgcacg cccttcgcgc
     3241 cttccggcag cgtgtagcgg tagggcgcgt cttccaccac gtggcggatg aagtcctccc
     3301 actgcacctt gaagccgttg tcgtagacct gggtgtccgg cacttcctgc cactgggcgg
     3361 ggaagttcat cgtctgcttc tggtccggat tccagaccgg gcgcggggta ttcacccggg
     3421 actggctgaa gcactgcgtg agcgtggcct gggccgaacc gtgggtgccg tcgacgtgga
     3481 aggtgaccag gtcgtcgcgc ttgacgcggg tggcccagga gctgttgacc tgggcgatca
     3541 cgtgctcgcc gttgtgaccc ttcagctcca gcgtcgcata ggcggcgtcg tcagcggtgg
     3601 cttcatacgg cctgccggat tcgtcccagc gcttcgggat gtgggtcgcc cccaggcagg
     3661 acaccgactg cacctcgccg aacaggttgt ccagcacata gcgccagtgg cacatcatgt
     3721 cgaggatcat gccgccgccg tcttccttgc ggtaattcca cgacgggcgc tggatcggct
     3781 gcagatcgcc ttcgaacacc cagtagccga actcgatgcg caccgccagc atgcggccga
     3841 agaagcccga gcggcgcagc atgtcgagct tgcgcaggcc gggcaggaac agcttgtcct
     3901 gcaccgcgcc gtgcttgagg cccgatttct gcgcgaggcg gcagatttcc accgcctcgt
     3961 tcaggttggt cgcgatcggc ttttcgcagt agacgtgctt gccggcgcga atcgcctgcg
     4021 cgagcagcgt cggacgcatc tgggtggtgc cggcgtcgaa gaagaccgtg tcgcccttgt
     4081 tggcgagcgc ttcgtccagg ttcgtgcccc agcgcgagat gccatgggcg cgcgccagtg
     4141 cctcgatctt ctcggcattg cggccgatca ggatcgggtc gggcatcacc ttgctgccgt
     4201 ccgagagcgt gacgccgccc tggttgcgga tcgccacgat cgagcggatc aggtgctggt
     4261 tcatgcccat gcgcccggtc acgccgtgca tgatgaggcc gagtcgttgt gtgctcatgt
     4321 tgcttttcct ggggttgtct tttgtcgggt gttattgcgg ctcgatgccg gcagccttga
     4381 cgatctgcgc gttggtgcgc acgtcgtcct tcaggtactg gtcccactgc tcgggcgaca
     4441 ggtgccagcc ttcggcgccg agcttggcga ggcgctcctg cacgtcgggc gaagcgagcg
     4501 ccttctttac ttccgcgttg agcttgctga cgatgtcacg cggggtcttg gcgggaacca
     4561 tcatgccgat ccagaagttg aattccgagc ccggcacgcc ggcctcgctg gtggtcggca
     4621 cgttcggcag caaggcggca cggttcggcg atccgaccgc aagcggcacc agccggccgt
     4681 cgctgaggaa gggcagcacc ggcgcgaccg gcgagaagta atagtcgacg cggccggtca
     4741 tcacttcggt caccgcttcc ggcgagccct tgaacggaat gtgcaccgcg tcgatcttcg
     4801 cctgggtacg gaacttctcg gcgttcagat gggtggcgct gccctgtccg gccgaagcga
     4861 agttcatctt gccgggattg gccttcgcat ggtcgagcag gtccttcagc gagcgcatgt
     4921 tcttgctcgg cgcgaccacc agcacgttcg gcagcgtcgc cagcggggtc acgccggcga
     4981 aatcgccgat cgcgtcgtag gtgagcttcg agtaggtcga cgggctgacc gtgtgcgagg
     5041 cggagtgaat cagtaccgtg tagccgtccg gcgcggcctg ggcaacctgg gcggcgccga
     5101 tggtgccgcc tgccccgccg cggttttcca cgatcaccgg ctggcccatg ctggtgctga
     5161 gcttttccgt gactacacgc gcgacaaggt cggttgtgct gcctgcggta aacggcacga
     5221 tcacgcggat cggcttgctc ggataggcgt tctgcgcaac ggctgaaaaa ctggccaggc
     5281 ccacgatcag gcccagaccc agagtccgga cggatttcat cgatgtctcc tcatcttgta
     5341 ttggaagaat gcggctaggt gctgctgtgg atcgctgttg ctcgtcaatt ctttattcac
     5401 cgtttggtga ataaacagat gctaggatat gcacatcgat gcgtcaaggg tccgttttcg
     5461 cgcaactctc gccaattttt cgattgttaa ttcaccattc ggataattat ggtgactggt
     5521 gtaaattcga gggcccgcat cgccgccacg cccggctcgg cgcgcccctc caaccagacg
     5581 aggaattcat gaccagcccg atcgatcccc gccgcctgct gctcgacatg tacgccagcg
     5641 cgg
//
LOCUS       DATLGF010000499         6388 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_78823,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000499 DATLGF010000000
VERSION     DATLGF010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6388
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78823"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1128
                     /locus_tag="VM406_15545"
     CDS             157..1128
                     /locus_tag="VM406_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVL77428.1"
                     /translation="MKLSTFAKSALLAAALAAGAAAHAQNYPVKPITFIVTYPPGGTV
                     DTVARIIAPKLSARLGQPVIVDNRGGAGGIIGGSVVAKSAPDGYTLMLDASNHSQNVA
                     LRGRMPFDTVKDFAPVSLLARVPNLIVATPGFPAKTVQELIAQAKAKPGAIEYASAGN
                     GSSPHLAGELFNMMAGTEMLHVPYKGGGPAMIDVMAGQVPLLFASAGSAMSHVKSGKL
                     RPIAVAAPQRIAALPEVPTATEAGPPGYVMYEWNAVFAPAGTPRPIIDRLANEFSAVL
                     KDPEVAARLEGIGMEVIGSTPEELDRFRKAEIEKWTDLGKKRKIRLD"
     gene            complement(1222..1297)
                     /locus_tag="VM406_15550"
     tRNA            complement(1222..1297)
                     /locus_tag="VM406_15550"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1262..1264),aa:Ala,seq:cgc)
     gene            1473..1655
                     /locus_tag="VM406_15555"
     CDS             1473..1655
                     /locus_tag="VM406_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF904 domain-containing protein"
                     /protein_id="HVL77429.1"
                     /translation="MQLAELTQSLRRENADLRLSLATKSAENADLSRRIDEAYQRVAA
                     LLEKLPKSDEANEEAV"
     gene            1652..1963
                     /locus_tag="VM406_15560"
     CDS             1652..1963
                     /locus_tag="VM406_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HVL77430.1"
                     /translation="MIQIDVSIMGTAYKLACREGEEAALRQAVQYLDERMCAIRDAGK
                     IKGNDRIAVMAALGIAAELLATKSQDGPFAGMALAEIKQKISAMHEVIDAALTSQENL
                     F"
     gene            2010..2188
                     /gene="ssrS"
                     /locus_tag="VM406_15565"
     ncRNA           2010..2188
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VM406_15565"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            2261..2719
                     /locus_tag="VM406_15570"
     CDS             2261..2719
                     /locus_tag="VM406_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HVL77431.1"
                     /translation="MQYWLMKSEPDEVSIDDALDAPNRIVPWTGVRNYQARNFMRDAM
                     QVGDGVLFYHSSCAEPGIAGIAEVASAPYPDPTQFDSRSKYYDPKSTPEQPRWVLVDV
                     RALRKTRLLSLPELREVPELAEMQVLRRGNRLSITRVTPAEWRCIIGLLK"
     gene            2737..3540
                     /locus_tag="VM406_15575"
     CDS             2737..3540
                     /locus_tag="VM406_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVL77432.1"
                     /translation="MDLYLVAMLLALGAAVGFAAGLLGIGGGMLLVPFMTMLLTARGF
                     ASEHIVHMAIATSLATIMFTSLSSVRAHHKRGAVLWPVAKLLAPGILIGAWIGPWLAA
                     QMNASMLALFFALFVAFSGTQMLLDRKPAPTRELPKAPGMFAAGGVIGVLSGIVGAGG
                     GFVSVPFMTWCNVKIHNAVATSAALGFPIALAGTLSYIYNGWSVPDLPAGALGFIYLP
                     ALVVIALASVATAPLGAKTAHALPVKTLKKVFAVLLYALAAYMLYKGLQ"
     gene            complement(3569..5077)
                     /locus_tag="VM406_15580"
     CDS             complement(3569..5077)
                     /locus_tag="VM406_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA synthase"
                     /protein_id="HVL77433.1"
                     /translation="MNANLYDLFASRFPADRNTCCIETHDGLYYSWNDLDRASAKLAN
                     LLAGLKLPPDARVAVQVEKSPEALLLYLATLRAGLVYLPLNTAYRAAEIEYFIDNAEP
                     AVVVCSPQNFGWVSQLAFKAGVKHVFTLGEDRQGSLLTRAALQSSRFETVARKPDDLA
                     AILYTSGTTGRSKGAMLTHENLASNARVLHDYWRWQDGDVLLHALPLFHVHGLFVASH
                     GALFNGSKMIFLPKFDSAEVLRQLPRATVFMGVPTYYVRLLADPAFTREACANMRLFV
                     SGSAPLLTETFNTFRERSGHTILERYGMSETTMLVSNPYDGERRAGTVGFPLPGVEVR
                     VVDGEGRPCRQGEIGDIQVRGPNVFKGYWRMPEKTAEEFTADGFFKTGDVGRFDADDY
                     LSIVGRSKDLIISGGYNVYPKEIESVIDEMDGVVESAVVGVPHPDFGEAVTAVIVPKP
                     SAARDEAGVIAYLKARIANFKVPKRVHFVDELPRNTMGKVQKNLLRERFGMG"
     gene            complement(5079..5852)
                     /locus_tag="VM406_15585"
     CDS             complement(5079..5852)
                     /locus_tag="VM406_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVL77434.1"
                     /translation="MSSVNVEVRGAIAYVTLSNPGKLNALSANMWRDLASAFKRLAED
                     APLRCIVVRGADGNFAAGADIEEFEQLRRTPEDGIRYHTELVGGALEAIASCPHPTVA
                     AIEGACVGGGLEIACACDLRIAAPSARFGIPINRLGFALAPLELQGLLALAGPAVARE
                     MLLEGRVFDAEEAKEKGLLHRIADDVHAEADATAQRIAHGAPLAARANKALIRRLTAS
                     AAPLSERELQDAFACLSSDDYHEGVQAFLEKRPPRFTGR"
     gene            complement(5855..>6388)
                     /gene="lgt"
                     /locus_tag="VM406_15590"
     CDS             complement(5855..>6388)
                     /gene="lgt"
                     /locus_tag="VM406_15590"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HVL77435.1"
                     /translation="LEIFAIWKGGMSFHGGFIGVLVGMWLWARRAGRTTIEVYDFIAP
                     LVPLGYTAGRIGNFINAELPGRPADPSLPWAMVWPNVDSIPRHPSPIYQALVDGLLVF
                     VIVWIYARKARPPLAVGAMFVGLYGAARFFTEYFRTPDYEVQFAGLTISAGQMLSLPM
                     IAFGILVMLYHARRRTA"
BASE COUNT         1157 a         2053 c         2084 g         1094 t
ORIGIN      
        1 ccagcatcgg cacctggtcg actaccagtc gaccagaacg gccctggaca gctggataga
       61 cgacgaagca acgcggcgca tcgtgctctg cgatacgcct gcctcgtttt tcaaattttg
      121 aagacagccg gctgaaccgg cacaatggag atacagatga agctcagcac tttcgcaaag
      181 tccgcgctgc ttgcggctgc cctggccgcc ggcgccgccg cccacgccca gaactatccg
      241 gtcaagccga tcaccttcat cgtcacctat ccgccgggcg gaaccgtcga tacggtcgcg
      301 cgcatcatcg cgcccaagct gtcggcccgc ctcggccagc cggtcatcgt cgacaaccgc
      361 ggcggcgctg gcggcatcat cggcggcagc gtcgtcgcca agagcgcgcc ggacggctac
      421 acgctgatgc tggacgcttc caatcattcg cagaatgtcg cgctgcgcgg ccgcatgccg
      481 ttcgatacgg tgaaggattt cgcgccggtc tcgctgcttg cccgggtgcc caacctgatc
      541 gtggctacgc ccggctttcc ggcaaaaacg gtgcaggaac tgatcgccca ggccaaggcc
      601 aagccgggcg caatcgaata cgcatccgcg ggcaacggct cctcgcccca cctcgccggc
      661 gaactgttca acatgatggc gggcaccgag atgctgcacg tcccgtacaa gggcggcggc
      721 ccggcgatga tcgacgtgat ggcgggccag gtcccgctgc tgttcgccag cgcgggatcg
      781 gcgatgtcgc acgtgaagag cggaaagctg cgtccgatcg ccgtcgcggc cccgcagcgc
      841 atcgccgcgc ttcccgaggt gcccacggcc accgaagccg gcccgccggg ctacgtgatg
      901 tacgaatgga acgcggtgtt tgcgccggcc ggcaccccgc gtccgatcat cgatcgcctg
      961 gcgaacgaat tcagcgcggt gctgaaagac ccggaagttg ccgcccgcct cgagggaatc
     1021 ggcatggaag tgatcggttc gaccccggaa gaactggacc gcttccgcaa ggcggagatc
     1081 gagaagtgga ccgatctcgg caagaagcgc aagatccgtc tcgactgagc ggtagcggaa
     1141 ggcgggaaga atatcgctga gtcaggggga gggcgcggca cgcgctttcc actgacaagc
     1201 acgcggcgat gaagggttca ttggtggagc ggaggaggat cgaactcccg accttcgcat
     1261 tgcgaacgcg acgctctccc agctgagcta ccgccccatg ggcagagatt ctattgactc
     1321 gcgtgcacct gcgcaagctc tcgcgcaaca gtcggcgaga tgacctgagg aatgttgccc
     1381 gagtgattga tccgatggcg taaaattgcc ggcgacgccc atcagtcacg gatgacatga
     1441 tttccgactt ccaacagctt tccgaaaagg tcatgcaact ggccgaactc acgcagtcgc
     1501 tgcgccggga gaacgccgac ctgcgcctgt cgctcgcgac caagagcgcc gagaacgccg
     1561 acctgtcgcg ccgcatcgac gaggcttacc agcgggtcgc ggcgctgctg gagaagctgc
     1621 cgaagtcgga tgaagccaac gaggaggccg tatgatccag atcgacgtct ccatcatggg
     1681 caccgcctac aagctcgcct gccgcgaggg cgaggaggcg gcgctgcgcc aggccgtgca
     1741 gtatctggac gagcgcatgt gcgcgatccg cgacgccggc aagatcaagg ggaacgaccg
     1801 catcgccgtg atggcggcgc tcggcatcgc ggccgaactg ctggcgacga agtcgcagga
     1861 tgggcccttc gccggcatgg cgctcgccga aatcaagcag aaaatttcgg cgatgcatga
     1921 agtgatcgac gcggctctca cgtcccagga aaatctgttc tgacgaccgg cgaaatgctt
     1981 tgacagccct ttcgattggg gtacacttag ggctcctgcc gtgttcgcga ttgccatata
     2041 ttccttgaac cattcacgtg cataggttgc ggaatctgtt tgatgggcga gtttcgtcgc
     2101 tagtccgatg aacccgaaat tgaactactg tgaccacctt gaaccgtaag gttcgggatg
     2161 ccggcaagga cggcacaggc ggggctgaac acacgagcgg ctgcatgaag atgcagccgc
     2221 tcttttttgt gcgcgcggct ttcgcaggag ggatacgacg atgcagtact ggctgatgaa
     2281 atccgaaccc gacgaagtca gcatcgacga cgcgctggac gcgccgaacc gcatcgtgcc
     2341 ctggaccggc gtgcgcaact accaggcgcg caatttcatg cgcgacgcga tgcaggtcgg
     2401 cgacggcgtg ctgttctacc actcgagctg cgccgagccc ggcatcgcag gcatcgccga
     2461 agtcgcgagc gcgccatatc ccgacccgac ccagttcgac tcgcgcagca agtactacga
     2521 tccgaagtcg acgccggaac aaccacgctg ggtgctggtc gacgtgcgcg cgctgcgcaa
     2581 gacccgcctg ctgagtctgc cggaactgcg cgaagtgccg gaactcgccg agatgcaggt
     2641 gctcaggcgc ggcaaccggc tttcgatcac ccgcgtcacg cctgcggagt ggaggtgtat
     2701 catcggcctc ctcaaataat ttcctgaagg catttcatgg atctttatct tgtcgcgatg
     2761 ctgctcgcgc tcggcgcggc ggtcggtttt gccgcaggcc tgctgggcat cggcggcggc
     2821 atgctgctgg tgccgttcat gacgatgctg ctcaccgcgc gcggtttcgc gagcgagcac
     2881 atcgtccaca tggcgatcgc gacctcgctg gcgaccatca tgttcacctc gctgtcttcg
     2941 gtgcgcgcgc atcacaagcg cggcgcggtg ctgtggccgg tggcgaagct gctcgcgccc
     3001 ggcatcctga tcggcgcctg gatcggcccc tggctggccg cgcagatgaa cgcctcgatg
     3061 ctggcgctgt tcttcgcgct gttcgtcgcc ttctccggca cacagatgct cctcgaccgc
     3121 aagccggcgc ccacgcgcga attgccgaag gcaccgggga tgttcgcggc cggcggcgtc
     3181 atcggcgtgc tgtcggggat cgtcggggcg ggcggcggtt tcgtgtccgt accgttcatg
     3241 acctggtgca acgtgaagat tcataacgcg gtcgccacca gcgcggcgct cggcttcccg
     3301 atcgcgctgg cgggcacgct ctcctacatc tacaacggct ggtcggtgcc ggacctgcct
     3361 gccggcgcgc tcggcttcat ctatctgccg gcgctggtcg tgatcgcact ggcgagcgtg
     3421 gcgacggcgc cgctgggcgc gaagaccgcg catgcattgc cggtgaagac gctgaagaag
     3481 gtatttgcgg tgctgctgta cgcgctggcg gcctacatgc tgtacaaggg cctgcagtaa
     3541 tcgggcgtta cctcatgccg cttcgggatc atcccatccc gaagcgctcc cgaagcagat
     3601 tcttctgcac cttgcccatc gtgttgcgcg gcagttcgtc gacgaagtgc acgcgcttgg
     3661 gcaccttgaa gttcgcaatc cgcgccttca ggtaggcaat cacgccggcc tcgtcgcgcg
     3721 cggcgcttgg cttcggcacg atgaccgcgg tgaccgcttc gccgaaatcc ggatgcggaa
     3781 cgccgaccac cgccgactcc accacaccat ccatctcgtc gatcaccgat tcgatttcct
     3841 tcggatagac gttgtagccg ccggagatga tcaggtcctt gctgcggccg acgatggaaa
     3901 gatagtcatc cgcatcgaaa cgtccgacgt cgccggtctt gaagaaaccg tcggcggtga
     3961 attcttctgc ggtcttctcc ggcatgcgcc agtagccctt gaacacgttc ggcccgcgca
     4021 cctggatgtc gccgatctcg ccttgccggc aggggcggcc ctcgccgtcg acgacgcgca
     4081 cctccacgcc cggcaatgga aagccgaccg tccccgcacg ccgttcgccg tcatagggat
     4141 tcgagaccag catcgtggtc tcgctcatcc cgtagcgctc gaggattgta tggccgctgc
     4201 gttcacggaa ggtattgaac gtctcggtca gcagcggcgc cgatccggag acgaacaggc
     4261 gcatgttcgc gcaggcctcg cgcgtgaatg cggggtcggc gaggaggcgc acgtagtagg
     4321 tcggcacgcc catgaagacg gtcgcgcgcg gcagctggcg cagcacctcg gcgctgtcga
     4381 atttcggcag gaagatcatc ttgctgccgt tgaagagcgc gccgtgcgag gcgacgaaca
     4441 ggccgtggac atgaaacagc ggcagtgcat gcagcagcac gtcgccatcc tgccatctcc
     4501 agtaatcgtg cagcacgcgc gcgttcgacg cgaggttttc atgcgtgagc atcgcaccct
     4561 tgctgcgccc ggtggtgccg gaggtgtaca ggattgcggc gaggtcgtcc ggcttgcgcg
     4621 caaccgtctc gaaacggctc gactgcagcg cggcgcgggt gagcaggctg ccctggcggt
     4681 cttcgccgag ggtaaacaca tgcttgacgc cggccttgaa cgcaagctgc gacacccagc
     4741 cgaagttctg cggtgaacac acgaccacag caggctcggc attgtcgatg aaatattcga
     4801 tctcggcggc gcgatacgcg gtattgagcg gcaggtagac caggccggcg cgcagcgtgg
     4861 ccagatacag cagcagcgct tccggcgact tttcaacctg cacggcgacg cgcgcatcgg
     4921 gcggcagctt cagcccggcc aagaggttcg caagcttcgc actggcccgg tcgagatcgt
     4981 tccacgagta gtacaggccg tcgtgggttt cgatgcagca ggtgttgcgg tcggcgggga
     5041 agcgggatgc gaagaggtcg tagagattgg cgttcatgtc agcggccggt gaagcgggga
     5101 gggcgttttt cgaggaaggc ttgcacgcct tcatggtaat cgtcgctcga caggcaggcg
     5161 aatgcatcct gcagttcacg ttcggagagc ggggccgcgg atgcggtcag gcgccggatc
     5221 agcgccttgt tcgcgcgggc cgcgaggggc gcgccatgcg caatccgttg cgctgtcgca
     5281 tcggcttccg catgcacgtc atcggcgatc cggtgcagca ggcccttctc cttggcctct
     5341 tccgcatcga acacgcggcc ttcgagcagc atttcgcgcg cgacggcggg ccccgccagt
     5401 gcgagcaggc cctgcaattc cagcggcgcg agcgcgaacc ccaagcggtt gatcggaatc
     5461 ccgaagcgcg ccgagggcgc agcgatgcgc aggtcgcagg cgcaggcgat ttccagtccg
     5521 ccgcccacgc aggcgccctc gatggccgcc accgtcggat gcggacagga tgcgatcgcc
     5581 tccagtgccc cgccgaccag ttccgtgtgg tagcggatgc cgtcttcggg ggtgcggcgc
     5641 agctgttcga actcctcgat atcggcgccg gcggcgaagt tcccgtcggc gccgcgcacg
     5701 acgatgcagc gcagcggcgc gtcttcggca aggcgcttga aggcgctggc gagatcgcgc
     5761 cacatgttcg ccgaaagcgc attgagcttg cccggattgg acagcgtcac atacgcgatc
     5821 gcgccccgga cttccacatt tactgaggac atgctcaggc ggtgcggcgg cgcgcgtgat
     5881 acagcatcac gaggatcccg aatgcgatca tcggcagcga cagcatctgg ccggcggaaa
     5941 tcgtgaggcc cgcgaactgc acctcatagt cgggcgtgcg gaagtattcg gtgaagaagc
     6001 gcgcggcgcc gtacaggccg acgaacatcg ctcccactgc aagcggcggc cgcgccttgc
     6061 gcgcgtagat ccatacgatc acaaagacca gcagaccatc caccagcgcc tggtagatcg
     6121 gcgacggatg acgcggaatg ctgtccacat tcggccagac catcgcccag ggcagcgaag
     6181 gatcggccgg tcggccgggc agttcggcat tgatgaaatt gccgatgcgt cccgctgtgt
     6241 agccgagcgg aaccagcggt gcgatgaaat catagacttc gatcgtggtc ctgccggcac
     6301 gccgcgccca tagccacatc ccgacgagca cgccgatgaa gccgccatgg aaggacatcc
     6361 cgcccttcca gatcgcgaag atctcgag
//
LOCUS       DATLGF010000500         5601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_218888, whole genome shotgun sequence.
ACCESSION   DATLGF010000500 DATLGF010000000
VERSION     DATLGF010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218888"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..1137
                     /gene="rfbB"
                     /locus_tag="VM406_15595"
     CDS             76..1137
                     /gene="rfbB"
                     /locus_tag="VM406_15595"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011886366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HVL77436.1"
                     /translation="MTLLVTGGAGFIGSNFVLDWLAGSDEPVVNLDKLSYAGNLDNLA
                     SLEHDPRHIFVRGDIGDSALVLELLNQHRPRAVLNFAAESHVDRSIDSPAAFIETNVV
                     GTFRLLESVRTYWRDLDDAARAAFRFLHVSTDEVFGSLEPNEPAFSERHQYQPNSPYS
                     ASKAGADHLVRAWHHTYGLPVLTTNCSNNYGPFHFPEKLIPLVIHNALAGKPLPIYGD
                     GKQVRDWLYVGDHCNAIRRVLQAGAPGETYNVGGHSEKTNLEVVETICAILDEIRPRE
                     DGESYRAQIRFVQDRPGHDRRYAIDTSKIERELGWRPSESFETGIRRTVQWFLDNGHW
                     VERATSGAYREWTERQYGL"
     gene            1134..2036
                     /gene="rfbD"
                     /locus_tag="VM406_15600"
     CDS             1134..2036
                     /gene="rfbD"
                     /locus_tag="VM406_15600"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HVL77437.1"
                     /translation="MKILLLGKDGQVGWELQRALAPLGTLVAAGRAQADLGEPERVAD
                     FIRAQAPDLIVNAAAYTAVDKAETDAHTAFRINAEAAGVIAAEAARAGAWLVHYSTDY
                     VFDGEQSAPYTETDAVNPLSVYGRSKLEGENLIRAHCPRHLIFRTCWVHAARGGNFAK
                     TMLRLATEREELKVVCDQFGAPTSAELIADVTALAVHRLLAQDDPAALAGTYHLAAAG
                     TTSWHGYAQYVLEQARERGMPVRVAPDSLLPIPAADYPTPARRPRNSMLDTTRLQQAF
                     QLQLPDWRHHVARLVAELAERGKP"
     gene            2033..2914
                     /gene="rfbA"
                     /locus_tag="VM406_15605"
     CDS             2033..2914
                     /gene="rfbA"
                     /locus_tag="VM406_15605"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HVL77438.1"
                     /translation="MTRKGIILAGGSGTRLHPATLAISKQLLPVFDKPMIYYPLSTLM
                     LAGIRDVLIISTPQDVPRFEQLLGDGAQWGMRLQYAVQPSPDGIAQAFVLGAEFLGDS
                     PAALILGDNIFHGHDLPAVLSSANARADGATVFAYHVDDPQRYGVVGFDANGMAVSLE
                     EKPAQPKSRYAVTGLYFYDNEVVELARTLKPSARGELEITDLNRIYLERAKLHVEIIG
                     RGYAWLDTGTHESLLEASQFIATLENRQGLKVSCPEEIAYRQKWIAAADLETLARRMA
                     NNRYGQYLLRLLDDKVF"
     gene            2914..3459
                     /gene="rfbC"
                     /locus_tag="VM406_15610"
     CDS             2914..3459
                     /gene="rfbC"
                     /locus_tag="VM406_15610"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HVL77439.1"
                     /translation="MQTTPLAIPDVLLIEPKVFGDARGHFFESYNQRRFEEALGRSVQ
                     FVQDNQSRSLRKVLRGLHYQIRQPQGKLVRVIHGEIFDVAVDLRRASPTFGKWVGAVL
                     SAENRHQIWIPEGFAHGFVTLSEQAEVLYKTTDYWAQEHERCIAWNDPDLAIDWPFAG
                     IPTLSAKDAAGVSFAGAETFD"
     gene            3666..4550
                     /locus_tag="VM406_15615"
     CDS             3666..4550
                     /locus_tag="VM406_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HVL77440.1"
                     /translation="MQSTRYVNISAYKFVTFDDTVEKRPLFREKCEELQLKGTILLSP
                     EGINLFLAGLREQIDAFLAWLRSDPRFADLEPKESLSDNQPFTKMLVKLKREIITMKN
                     PLIRPEDGRAPAVDAKTLKRWLDQGHDDNGRPVVMVDTRNAFEVDVGTFENTIDYRID
                     KFSEFPEVIARQKDELADKTVVTFCTGGIRCEKAAIHMQNVGFNSVYQLEGGILKYFE
                     EVGGAHYRGDCFVFDYRTALSPSLEPTETKQCFACRAVVTPQEQLSPWYVAGESCPHC
                     KKMRPGHNATTAATPAAD"
     gene            4588..5481
                     /gene="gluQRS"
                     /locus_tag="VM406_15620"
     CDS             4588..5481
                     /gene="gluQRS"
                     /locus_tag="VM406_15620"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HVL77441.1"
                     /translation="MTGYVGRFAPSPTGPLHLGSLVAALASYLDARLHRGRWLVRIED
                     VDEARTVPGAAEAILACLQRFGMHWDGEVLWQSARTSRYEAAFERLGEHAYPCGCTRR
                     EIADSRIAVASDGAAVYPGTCRGGLAPGKSARAFRLRVPDGEASTITFEDRWMGRVTQ
                     RLDREVGDFVLRRADGFWAYQLAVVVDDAEQGVTHVVRGADLLDSTPRQIYLQRLLGY
                     PTPAYLHVPVVVNAEGEKLSKQTGAAALDPDEPLPQLMRAARFLNLDVGEPGSLPEFW
                     RHAVPAWAAALASRSSARSGG"
BASE COUNT         1036 a         1949 c         1732 g          884 t
ORIGIN      
        1 ctccccgcct cgtataatgt gcccctgcca cccacgcggc ccgacccgcg caaagaacaa
       61 cccagcaact gcctcatgac cctactcgtt accggcggcg ccggattcat cggctcgaat
      121 ttcgtcctcg actggctggc cggctcggat gaaccggtcg tcaacctcga caagctctcc
      181 tacgccggca acctggacaa tcttgcaagc ctcgagcacg acccgcgaca catcttcgtg
      241 cgcggcgaca tcggcgattc cgcgctggtg ctcgaattgc tgaaccagca tcgcccgcgc
      301 gcggtgctca atttcgccgc cgaaagccat gtggaccgct cgatcgactc gccggccgcc
      361 ttcatcgaaa ccaatgtcgt cggcaccttt cgcctgctgg aatcggtccg cacctactgg
      421 cgcgatctcg acgacgccgc gcgcgccgcg ttccgcttcc tgcacgtttc caccgacgaa
      481 gtgttcggct cgctcgagcc gaacgagccg gccttcagcg agcgtcatca gtaccagccc
      541 aacagcccct actccgcaag caaggccggc gccgatcact tggtgcgcgc ctggcaccac
      601 acctacggct tgccggtcct gacgacgaac tgctcgaaca actacggccc tttccacttc
      661 cctgaaaagc tgatcccgct ggtgatccac aatgcgctgg ccggcaagcc gctgccgatc
      721 tacggcgacg gcaagcaggt gcgcgactgg ctctatgtcg gcgatcactg caacgcgatc
      781 cgccgcgtgc tgcaagcggg cgcgccgggc gagacctata acgtcggcgg gcacagcgaa
      841 aagaccaatc tggaagtggt ggaaaccatc tgcgccatcc tcgacgaaat ccgcccgcgc
      901 gaggacggcg agagctaccg cgcgcagatc cgcttcgtgc aggaccgccc cggccacgac
      961 cgccgatacg cgatcgacac gtcgaagatc gaacgcgaac tcggctggcg cccaagcgaa
     1021 agtttcgaga ccggcatccg ccgcacggtg caatggttcc tcgacaacgg acactgggtg
     1081 gagcgcgcca cctccggcgc ctatcgcgag tggacggaaa ggcagtacgg cctgtgaaga
     1141 tcctcctgct cggaaaggac ggccaggtcg gctgggagct gcagcgcgcg ctggcgccgc
     1201 tgggcacgct ggtagcagcc ggtcgcgccc aggcggacct gggcgaaccg gaacgcgtgg
     1261 ccgatttcat ccgcgcacag gcgcctgact tgatcgtgaa cgcggcggcc tacaccgccg
     1321 tcgacaaggc cgaaaccgac gcgcacaccg cattccggat caatgccgaa gccgcgggcg
     1381 tgatcgccgc cgaggcggcc cgcgccggcg catggctggt ccactattcg accgactacg
     1441 tgttcgacgg cgaacagtcc gcgccctata ccgaaaccga tgccgtcaac ccgctctcgg
     1501 tctacggccg cagcaagctc gaaggcgaga acctgatccg cgcgcactgc ccgcgccatc
     1561 tgattttccg cacctgctgg gtgcatgcgg cgcgcggcgg caatttcgcg aagaccatgc
     1621 tgcgcctggc gaccgagcgc gaggaactga aggtcgtgtg cgaccagttc ggcgcgccca
     1681 ccagcgccga gctgatcgcc gacgtgaccg cgctggccgt gcaccggctg ctggcacagg
     1741 acgaccccgc cgcgctcgcc ggcacctatc atctcgccgc cgccggcacc accagctggc
     1801 acggctatgc gcagtacgtg ctcgagcagg cgcgcgaacg cggcatgccg gtacgcgtcg
     1861 cgcccgacag tctgctgccg attccggcgg cggactaccc gacgccggcg cgccgtccgc
     1921 gcaattcgat gctcgacacc acgcgcctgc aacaggcgtt ccagctccag cttcccgact
     1981 ggcgccacca cgtcgccagg ctggtcgccg aactcgccga acgaggaaaa ccatgacgcg
     2041 caaaggcatc atcctggccg gcggcagcgg cacccgcctg cacccggcca cgctggcgat
     2101 ctccaagcag ctactgccgg tgttcgacaa gccgatgatc tactacccgc tcagcacgct
     2161 gatgctggcc gggatacgcg atgtgctgat catctccacg ccgcaggatg tgccgcgctt
     2221 cgaacagctg ctcggcgacg gcgcgcaatg gggcatgcgc ctgcagtacg cggtgcagcc
     2281 ttcgccggac ggcatcgccc aggccttcgt gctcggcgcc gaattccttg gcgacagccc
     2341 ggccgcgctc atcctcggcg acaacatctt ccatggccac gacctgcccg cggtgctctc
     2401 ttccgccaat gcacgcgccg acggcgccac cgtgttcgcc taccacgtcg acgacccgca
     2461 gcgctatggc gtggtcggct tcgacgccaa cggcatggcg gtcagcctcg aggaaaaacc
     2521 ggcacagccg aaatcgcgct atgccgtgac cggactttat ttctacgaca acgaagtcgt
     2581 cgaactcgcg cgcacgctca agccgtccgc acgcggcgaa ctcgagatca ccgacctgaa
     2641 ccggatctac ctcgagcgcg cgaaactgca tgtggaaatc atcggccgcg gctacgcctg
     2701 gctcgatacc ggcacgcacg aatcgctgct cgaagccagc cagttcattg cgacgctgga
     2761 aaaccgccaa ggcctgaaag tgtcctgccc cgaggaaatc gcctaccgcc agaagtggat
     2821 agccgcggcc gacctcgaga cgctcgcgcg ccgcatggcc aacaaccgct acggccagta
     2881 tctgctgcgc ctgctcgacg acaaggtgtt ctgatgcaaa ccaccccgct tgcgatccct
     2941 gacgtgctgc tgatcgagcc gaaggtgttc ggcgatgcgc gcggccattt cttcgaaagc
     3001 tacaaccagc gccgcttcga ggaagcgctg ggccgttccg tgcagttcgt gcaggacaac
     3061 cagtcgcgct cgctgcgcaa ggtgctgcgc ggcctgcact accagatccg ccagccgcag
     3121 ggaaagctgg tgcgcgtgat ccatggcgag atcttcgacg tcgcggtcga cctgcgccgc
     3181 gcgtcgccga ccttcggaaa atgggtcggc gccgtgctca gcgcggagaa ccggcatcag
     3241 atctggattc ccgaaggctt cgcgcacggc ttcgtcacgc tctcagaaca ggccgaggtg
     3301 ctgtacaaga ccaccgacta ctgggcgcag gagcacgagc gctgcatcgc ctggaacgac
     3361 ccggatctcg cgatcgactg gccgttcgcc ggtatcccga ccctgtccgc caaggacgcg
     3421 gccggggtgt cgttcgcggg cgccgaaacc ttcgattagc gtggcaggcg catataattg
     3481 cgccgaacca tgccggtagc agcgactcac accggcgtac caagaacaaa ccccttcccc
     3541 ctcccagggg aaggttggga tgggggtaca gagccccaac aggcggcgct gccccaatga
     3601 gcttcgcacc ccctccctaa ccctccccct gacaggggga gggaacgctt ccataccgac
     3661 atccgatgca atccacccgc tacgtcaaca tctccgccta caaattcgtc accttcgacg
     3721 acaccgttga aaagcgcccg ctgttccgcg agaagtgcga ggaactgcag ctcaagggca
     3781 cgatcctgct cagcccggaa ggcatcaacc tcttcctcgc cggcctgcgc gagcagatcg
     3841 atgcattcct tgcatggctg cgcagcgatc cgcgctttgc cgatctggaa ccgaaggaaa
     3901 gcctgtccga caaccaaccc ttcaccaaga tgctggtcaa gctcaagcgc gagatcatca
     3961 cgatgaagaa tccgctgatc cgccccgagg acggccgcgc gccggcggtg gacgcgaaga
     4021 ccctgaagcg ctggctcgac caaggccatg acgacaacgg caggccggtc gtcatggtcg
     4081 acacgcgcaa cgcgttcgaa gtggacgtcg gtacctttga gaacaccatc gattaccgca
     4141 tcgacaagtt cagcgaattc ccggaagtga tcgcccggca aaaggacgaa ctggccgaca
     4201 agaccgtggt caccttctgc acgggcggca ttcgctgcga gaaagccgcg atccacatgc
     4261 agaacgtcgg cttcaacagc gtgtaccagc tcgaaggggg gatcctgaag tacttcgagg
     4321 aagtgggcgg tgcgcactac cggggcgact gcttcgtctt cgactaccgc accgcgctca
     4381 gtccgagcct ggagccgacc gagacgaagc agtgcttcgc ctgccgggcg gtggtgacgc
     4441 cgcaggagca gttgtcgccg tggtatgtgg cgggggaatc gtgtccgcac tgcaagaaga
     4501 tgcggcctgg ccacaatgcg actactgctg ccactcccgc ggcagactga tgaacaagcg
     4561 ccgggccgcg cgaaccggag tgcaagcatg actggctatg tcggtcgatt cgccccatcg
     4621 cctaccggcc cgttgcacct aggctcgctc gtggccgcgc tggccagtta tctcgatgcg
     4681 cggctacacc ggggacgctg gctcgtgcgc atcgaggatg tcgatgaagc gcgtaccgtg
     4741 cccggcgccg ccgaagcgat cctcgcttgc ctgcaacgct tcggcatgca ttgggatggc
     4801 gaagttctct ggcaaagtgc ccgcacgtcg cgctatgaag cggcgttcga acggctcggc
     4861 gaacacgcct acccttgcgg ctgcacccgg cgcgaaatcg ccgattcacg cattgcggta
     4921 gcgagcgacg gcgccgccgt ctatccgggc acctgccgcg gcggactggc tccgggaaag
     4981 tcggcgcgcg cgttccggct gcgggtgcct gacggcgagg ccagcacgat caccttcgag
     5041 gatcggtgga tggggcgggt cacgcagcga ctggatcgcg aagtcggcga tttcgtgctc
     5101 aggcgcgccg acggtttctg ggcctaccag cttgcggtcg tcgtggatga tgccgaacag
     5161 ggcgtgaccc acgtcgtgcg cggtgcggac ctgctggact cgacgcctcg gcagatctac
     5221 ctgcaacgcc tcctcgggta tccgacgccc gcatacctgc acgtgccggt cgtcgtcaat
     5281 gccgaaggcg agaaactatc caagcagact ggggccgcgg cgctggatcc tgatgaaccg
     5341 ttgccgcaat tgatgcgggc tgcccggttc ctgaacctcg atgtgggaga gccggggtcg
     5401 ctgccggaat tctggaggca tgctgtgccg gcatgggcgg cggcactggc gtcccgcagc
     5461 agcgcgagat caggcggctg agtcgctgcc gcaggatacc gggcgggaaa gcggtcggtt
     5521 caacgcttcg gcacgccgcc gagaagcgcc gccaccttgc ttttctgcgg cttcacaggc
     5581 gggacctgct tgccatcctc t
//
LOCUS       DATLGF010000501         3094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_203237, whole genome shotgun sequence.
ACCESSION   DATLGF010000501 DATLGF010000000
VERSION     DATLGF010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203237"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1265)
                     /gene="leuC"
                     /locus_tag="VM406_15625"
     CDS             complement(<1..1265)
                     /gene="leuC"
                     /locus_tag="VM406_15625"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HVL77442.1"
                     /translation="MARTLYDKLWDAHVVHTEEDGTAVLYIDRHLVHEVTSPQAFEGL
                     KLAGRKPWRISANLAVADHNVPTTDRAEGIADPVSRLQVETLDANTREYGLTYFSMRD
                     KRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGCLAHGIGTSEVEHVLATQTLLA
                     KKSKSMLVQVDGTLPAGVTAKDIVLAVIGKIGTAGGTGYAIEFAGSTIRGLSMEGRMT
                     VCNMAIEAGARAGMVAVDDTTINYVKGRPFSPVGPHWDRAVEYWRTLHSDPGAKFDMV
                     VTLNAAEIKPQVTWGTSPEMVVAIDDRVPDPDKEKDAVKRDAMEKALVYMGLQPNTAI
                     SDIRIDKVFIGSCTNSRIEDLRAAAAVVRGKYRASNVKLAMVVPGSGLVKEQAEREGL
                     DKIFKDAGFEWREPGCSMCLAMNADRLEPG"
     gene            complement(1389..2213)
                     /locus_tag="VM406_15630"
     CDS             complement(1389..2213)
                     /locus_tag="VM406_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HVL77443.1"
                     /translation="MKTIELMKRVVLAVSLVMLAACETVQTTQGGVVGVERTQRMAVA
                     PEAIEQAATQQYGEVIAQARQKGVLNRNAAQVERVRAISQRLIPQTVHFRPDAKRWNW
                     EVNVLTSNEVNAWCMPGGKIAVYTGLIERLNATDDELAAVIGHEIAHALREHARERAS
                     EQMVASGVIAVGSALLGLGDIGQKGAEFAYMGLLGLPNSRRHEVEADRIGVELAARAG
                     YDPRAAISLWQKMGQIGGADIPAFLSTHPPRAERLNDLTVYSERVMPLYQEARRSR"
     gene            complement(2210..>3094)
                     /locus_tag="VM406_15635"
     CDS             complement(2210..>3094)
                     /locus_tag="VM406_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL77444.1"
                     /translation="AHFFFLMVALNLFTSAQGPLSEALMLSEMRGDLTHYGRLRLWGS
                     VGFILSVSLAGPLLDRHGVEAMPWIALSLLALVTAASLRMRESRPLHGARHAVSVASL
                     LRRRDVQAFFVSTFLMLAAHAALYVYYSLYLAQIGYSNTVIGLMWSLGVVVEIVFFFY
                     QTPLFRRFGVARLMVASLVLAVARFLLIGFGAESIALLLLAQVLHAATFGVHHSASIA
                     TLQRWFSGPLQARGQALYTSISYGLGGSLGALLLSPLWDTFGAPAVYLVASVLAAGGA
                     IAAVLCYRWQAVQTEENA"
BASE COUNT          551 a         1040 c          991 g          512 t
ORIGIN      
        1 cccggctcca gccggtcggc gttcatcgcc aggcacatcg agcagcccgg ttcgcgccac
       61 tcgaagccgg cgtccttgaa gatcttgtcc agcccctcgc gctcggcctg ctccttgacc
      121 agcccggaac cgggcaccac catcgccagc ttcacgttcg aggcgcggta cttgccgcgc
      181 acgaccgcgg ccgccgcgcg cagatcctcg atgcgggagt tcgtgcagga accgatgaat
      241 accttgtcga tgcggatgtc cgagatcgcg gtgttcggct gcaggcccat gtagaccagc
      301 gccttctcca tcgcgtcgcg cttgacggcg tctttttcct tgtcgggatc gggcacgcgg
      361 tcgtcgatcg ccaccaccat ttccggcgac gtgccccatg tgacctgcgg cttgatttcg
      421 gccgcgttca gcgttacgac catgtcgaac ttcgcacccg gatcggagtg cagcgtccgc
      481 cagtactcga ccgcgcggtc ccagtgcgga ccgaccgggg agaacggccg ccccttcaca
      541 tagttgattg tggtgtcgtc gaccgcgacc atgccggcgc gcgcgcccgc ctcgatcgcc
      601 atgttgcaca cggtcatgcg gccttccatc gacagcccgc ggatcgtgga accggcgaac
      661 tcgatcgcgt agccggtacc gccggccgtg ccgatctttc caatcaccgc cagcacgatg
      721 tccttggcgg tcacgccggc cggcagggtg ccgtcgacct ggaccagcat ggatttggat
      781 ttcttcgcca gcagcgtctg tgtggcgagc acgtgctcga cctcggaggt gccgatgccg
      841 tgggccaggc agccgaacgc gccatgggtg gaggtgtgcg agtcgccgca gaccacggtc
      901 atgccgggca gcgtcgcgcc ctgctccggc ccgatcacgt ggacgatgcc ctggcgcttg
      961 tcgcgcatgc tgaagtaggt caggccgtat tcacgggtat tggcgtccag ggtttcgacc
     1021 tgcaggcgcg agaccggatc ggcgatcccc tcggcgcggt cggtggtcgg cacgttgtgg
     1081 tcggccaccg cgagattggc agagatacgc cacggcttgc gcccggccag cttcaggccc
     1141 tcgaacgcct gcgggctcgt gacttcgtga acgagatgac gatcgatgta gaggacggcg
     1201 gtaccgtctt cctcggtgtg gacgacatgc gcgtcccaga gtttgtcgta aagcgtgcgg
     1261 gccatggcaa agagcgtgcg tgctgcgaaa acagggcgta atcccgggaa agaactcccg
     1321 attatgccat agcagcacgc cggcacggcg ggcacgagaa acgtgcccgg ccacggcccc
     1381 ggaaaggatc atcgggagcg gcgcgcttcc tggtagagcg gcatcacgcg ctcggaatac
     1441 acggtgaggt cattgagccg ctcggcgcgc ggcgggtggg tcgagaggaa ggccgggatg
     1501 tcggccccgc cgatctgtcc cattttctgc cacagcgaga tcgccgcacg cgggtcgtag
     1561 ccggcgcgcg ccgccagttc gacgccgatg cggtccgcct ccacttcatg gcggcgcgaa
     1621 ttcggcaaac cgagcaggcc catgtaggcg aactcggcgc ccttctggcc tatgtctccc
     1681 aggcccagca gggccgaacc gaccgcgatc acgccgcttg cgaccatctg ttccgacgcc
     1741 cgttcgcgcg catgctcgcg cagcgcatga gcgatttcat gtccgatcac cgccgccagt
     1801 tcgtcatcgg tcgcattcag gcgttcgatc aagccggtgt agaccgcgat cttgcctcct
     1861 ggcatgcacc aggcattgac ctcgttggag gtcagcacgt tcacctccca gttccagcgc
     1921 ttcgcatcgg ggcgaaagtg cacggtctgc ggaatcaggc gctggctgat cgcgcggacc
     1981 cgctcgactt gcgcggcgtt gcggttcaac acccccttct ggcgcgcctg ggcgatcacc
     2041 tcgccgtatt gttgcgtggc tgcctgctcg atcgcctccg gagccactgc catgcgctgg
     2101 gtgcgctcga cgccgaccac cccgccctgc gtcgtctgta cggtttcgca ggcggcaagc
     2161 atcaccagtg acaccgccag gactactcgc ttcatgagct caatcgtttt catgcgttct
     2221 cctcggtctg aaccgcctgc cagcggtaac acaagactgc ggcaatggca ccgcccgcag
     2281 ccaggacgga tgccaccaga tagactgcgg gcgcaccgaa tgtatcccag agcggactca
     2341 gcagcaacgc gcccagggag ccgccgaggc catatgaaat gctcgtgtaa agcgcctgcc
     2401 cccgcgcttg cagcgggccg gaaaaccagc gctgaagcgt ggcgatcgac gccgaatggt
     2461 gcacgccgaa ggttgccgcg tgcaatacct gggcaagcag caatagcgcg atggactccg
     2521 caccgaagcc gatcagcagg aagcgcgcca ccgccagcac caggctggcc accatcagcc
     2581 tggccacgcc gaagcgccgg aacagcgggg tctggtagaa gaaaaagaca atctcgacca
     2641 ccacgcccag cgaccacatc aaacctatca cggtattgct gtagccgatt tgcgccaagt
     2701 acagcgagta atacacatac agcgccgcat gcgctgccag catcaggaag gtcgacacga
     2761 agaatgcctg gacgtcgcgc cggcgcagga ggctggccac cgataccgca tgccgtgcgc
     2821 cgtgcagcgg ccgggactcg cgcattcgca ggctggccgc cgttacgagc gcgaggagtg
     2881 agagtgcaat ccatggcatc gcctcgaccc cgtgacggtc gagcaggggt cccgcgagcg
     2941 agacggaaag gatgaatccg accgagcccc aaaggcgcag gcggccgtaa tgggtcaggt
     3001 cgccgcgcat ctccgacagc atcagcgctt cggagagcgg gccctgggcg cttgtgaaca
     3061 aattcagcgc caccatgagg aagaagaaat gcgc
//
LOCUS       DATLGF010000502         3871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_219031, whole genome shotgun sequence.
ACCESSION   DATLGF010000502 DATLGF010000000
VERSION     DATLGF010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219031"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /gene="hscB"
                     /locus_tag="VM406_15640"
     CDS             complement(<1..431)
                     /gene="hscB"
                     /locus_tag="VM406_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018057880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HVL77445.1"
                     /translation="MQNHFELFHLPQQFTIDTAALDAAYREVQNRVHPDKFAGATDTE
                     KRVAMQWATRANEAYQTLKNPFKRAAYLCELNGVDLQTESNTAMPPAFLMQQMEWREA
                     LDDARSGRDLDALEALEADVRATRQQQIAQIGEQLDRGDYEA"
     gene            complement(505..828)
                     /gene="iscA"
                     /locus_tag="VM406_15645"
     CDS             complement(505..828)
                     /gene="iscA"
                     /locus_tag="VM406_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HVL77446.1"
                     /translation="MAITLTEKAAKHINRYIERRGKGLGLRFGVRTTGCSGLAYKLEY
                     VDEAAPEDQVFESHGVKVFVDPKSLPYIDGTELDFAREGLNEGFKFSNPNVKDECGCG
                     ESFRI"
     gene            complement(886..1287)
                     /gene="iscU"
                     /locus_tag="VM406_15650"
     CDS             complement(886..1287)
                     /gene="iscU"
                     /locus_tag="VM406_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HVL77447.1"
                     /translation="MSYSEKVLDHYENPRNVGVFEKGDDTVGTGMVGAPACGDVMRLQ
                     IKVGANGVIEDAKFKTYGCGSAIASSSLVTEWVKGKTLDEALSIKNTQIAEELALPPV
                     KIHCSILAEDAIKAAVQDYKAKHGTEATQAA"
     gene            complement(1318..2571)
                     /locus_tag="VM406_15655"
     CDS             complement(1318..2571)
                     /locus_tag="VM406_15655"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HVL77448.1"
                     /translation="MNAPLEKSLIDTIKAPHFPIYMDYSATTPIDPRVADKMIPYLRE
                     QFGNPASRSHMYGWSAEKAVEEARAHVAALVNADPREIIWTSGATEGNNLALKGAAHF
                     YKTKGKHIITVKTEHKAVLDTVRELERQGFEATYLQPQDNGLITLQQLEEAIRPDTIL
                     VSVMLVNNEIGVIQPVPEIGELCRAKGIIFHCDAAQATGKVHIDLQKWKVDLMTFTAH
                     KTYGPKGVGALYVRRKPRVRIEAQMHGGGHERGLRSGTLPVHQIVGMGEAFRIAKEEM
                     DEELGRIKALRDRLAKGLLEIEEVYVNGDMEHRVPHNLNVSFNYVEGESLIMAIKDIA
                     VSSGSACTSASLEPSYVLRALGRSDELAHSSIRFTIGRFTTEDDIDFAVNLIKTKVAK
                     LRELSPLWDMYKDGIDLSTIQWAAH"
     gene            complement(2590..3063)
                     /gene="iscR"
                     /locus_tag="VM406_15660"
     CDS             complement(2590..3063)
                     /gene="iscR"
                     /locus_tag="VM406_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HVL77449.1"
                     /translation="MRLTTKGRFAVTAMIDLALRQDNGPVTLSGISQRQEISLSYLEQ
                     LFGKLRRHEIVDSVRGPGGGYMLARRAQDVTVADIIIAVDEPIDATQCGGKENCGSGS
                     HEHGTRCMTHDLWATLNAKMVEYLDSVSLQDLVDQQRQKQAEHNVVLMPRPHVAA"
     gene            complement(3307..>3871)
                     /locus_tag="VM406_15665"
     CDS             complement(3307..>3871)
                     /locus_tag="VM406_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77450.1"
                     /translation="VEGTATVPREPDAMREEPRFTSRRARLSYQPLPGLVLQFSRGTW
                     SRIDQLVAEGQIRRTSLSATYTRNLTGGEWLTTLAWGRNAPRGGTSLNGYLAETSVRL
                     AGVHTAFARFEQVGSGPLYDPDTPFRMAAKVNKLSLGYYQDMYQRGANRIGLGVVASR
                     HLLSSAQEASIGRDPSSVMFFMRLRMH"
BASE COUNT          646 a         1238 c         1184 g          803 t
ORIGIN      
        1 gcctcgtagt cgcctcggtc aagctgctcg ccgatctgcg cgatctgctg ttggcgggtc
       61 gcgcgcacat cggcttcgag cgcttccagc gcatcgagat cgcggccgga ccgggcatca
      121 tcgagcgcct cgcgccattc catttgctgc atcaggaacg ccggcggcat cgcggtgttg
      181 gattcggtct gcaggtcgac gccgttcagt tcgcacagat aggcggcgcg tttgaacggg
      241 ttcttcagcg tctgatacgc ctcgttggca cgcgtggccc attgcatcgc cacccgcttt
      301 tcggtatccg tggctcccgc gaacttgtcc ggatgcacgc ggttctgcac ttcccggtac
      361 gcggcatcga gcgcggccgt gtcgatcgtg aattgctgcg gcaggtggaa cagttcgaag
      421 tggttctgca tggctaaaat aaaagcctgt ccgactgcaa caggcttgtt tgaatccgct
      481 ccagtcggga cgaattccga ttgttcagat gcggaagctc tccccgcaac cgcactcgtc
      541 ctttacgttc gggttgctga acttgaatcc ttcgttcaag ccctcgcgcg caaagtcgag
      601 ctcggtaccg tcgatatacg gcaggctctt cggatcgacg aagaccttca cgccgtgcga
      661 ctcgaacacc tgatcctccg gcgccgcctc gtcgacatac tcgagcttgt acgccagacc
      721 cgagcagccg gtcgtgcgca cgccgaagcg caggccgagg cccttgccgc gccgttcgat
      781 gtagcggttg atgtgcttgg ccgctttttc ggtgagtgtg attgccatgg tttcctgttg
      841 ctttggctat gcgcctgcgt cggggcggac gaacgcggcg aagaatcagg ccgcctgtgt
      901 cgcttccgtc ccgtgcttgg ccttgtaatc ctgcacggcg gccttgattg catcctcggc
      961 cagaatcgag cagtgaatct tcaccggcgg cagcgccagt tcttccgcaa tctgcgtgtt
     1021 cttgatggac agcgcctcgt cgagcgtctt gcccttcacc cattcggtca ccagcgagga
     1081 cgacgcgatc gccgaaccgc aaccgtaggt cttgaacttg gcgtcttcga tcacgccatt
     1141 cgcgccgacc ttgatctgca ggcgcatcac gtcgccgcac gccggcgcgc caaccatccc
     1201 ggtgccgacc gtgtcgtcgc ccttctcgaa gacgccgacg ttgcggggat tttcatagtg
     1261 gtcgagaact ttttccgagt aagacatgtt tgctgctcct tatagctatc ggcgcgctca
     1321 atgcgctgcc cactggatcg tcgacaggtc gatcccatcc ttgtacatat cccacagcgg
     1381 cgacagctcg cgcagcttcg ccaccttggt cttgatcagg ttcacggcga aatcgatgtc
     1441 atcctccgtc gtgaaccggc cgatcgtgaa gcggatcgag ctgtgcgcca gctcgtcgct
     1501 gcggcccagt gccctcagca cataagatgg ttccaggctc gccgaagtac aagcggaacc
     1561 ggaagatacc gcgatgtcct tgatcgccat gatcagcgac tcgccttcga cgtagttgaa
     1621 gctcacgttc aggttgtgcg gaacgcgatg ctccatgtcg ccattgacat agacctcctc
     1681 gatctcgagc aggcctttcg ccaggcggtc gcgcagggcc ttgatgcggc cgagctcctc
     1741 gtccatttct tccttggcga tccggaacgc ctcgcccatg ccgacgatct ggtgcaccgg
     1801 cagtgtgccg gagcgcaggc cgcgctcgtg cccgccgcca tgcatctgcg cctcgatgcg
     1861 cacgcgcggc ttgcggcgca cgtacagcgc gccgaccccc ttcggaccat aggtcttgtg
     1921 ggcggtgaag gtcatcaggt cgaccttcca cttctgcagg tcgatgtgca ccttgccggt
     1981 cgcctgagcg gcgtcgcaat ggaagatgat gcccttggcg cggcacagtt cgccgatttc
     2041 cggtaccggc tggatcacgc cgatctcgtt gttgaccagc atcaccgaca ccaggatcgt
     2101 gtccgggcgg atcgcctctt ccagctgctg cagcgtgatc aacccgttgt cctgcggctg
     2161 caggtaggtg gcctcgaagc cctggcgctc cagttcgcgc acggtgtcga gcaccgcctt
     2221 gtgctcggtc ttgaccgtga tgatgtgctt gcccttggtc ttgtagaagt gggcggcgcc
     2281 cttcagcgcg aggttgttgc cctcggtcgc gcccgaagtc cagatgatct cgcgcggatc
     2341 cgcgttgacc agcgccgcca catgggcacg cgcctcttcg accgccttct cggcgctcca
     2401 gccgtacata tggctacgcg atgccggatt gccgaactgc tcgcgcaggt acggaatcat
     2461 cttgtcggcc acgcggggat cgatcggcgt ggtcgccgaa tagtccatgt agatcgggaa
     2521 atgcggcgcc ttgatcgtgt cgatcaggct tttctccagg ggggcgttca tcgttctcta
     2581 tagctccagt caggcggcga cgtgcgggcg aggcatcagc accacgttgt gttcggcttg
     2641 cttctgccgc tgctgatcca cgagatcctg cagcgacacg gaatccagat attcgaccat
     2701 cttcgcgttc agcgtggccc acaggtcatg cgtcatgcag cgcgttccat gctcgtggct
     2761 gccactgccg cagttctcct tgccgccgca ctgcgtcgca tcgatcggct cgtcgacggc
     2821 gatgatgatg tcggcgacgg tgacatcctg cgcgcgccgc gcaagcatgt agccgccgcc
     2881 cgggccgcgc acggaatcca cgatctcgtg ccggcgcagc ttgccgaaca actgctcgag
     2941 gtaagacagc gaaatctcct gacgctggct gatcccggaa agcgtgacag gaccattgtc
     3001 ctggcgcagc gcgagatcga tcatcgcagt gacggcaaag cggccttttg tagtcagacg
     3061 catggtgact cttcccttga tgacgggccc aaccgaacga acatcgggaa atacccgaag
     3121 atttttgtcg agtatatcaa agttgagtga attcgtcagg tattccgccg ggcgggaaat
     3181 gtttcggcga ggaaaatggc tattatttca cactgcgccg caacaaggcg ggacgggagc
     3241 gattcgcccg cccgcagggc ctttgcgtga gggcaggccg accaaatatc ggcaccggag
     3301 gccggttcag tgcatgcgca ggcgcatgaa gaacatgacc gagctcggat cgcgaccaat
     3361 actcgcctcc tgcgccgagc taagcaggtg tcggctcgcc acgactccca ggccgatacg
     3421 gttggcgccg cgctggtaca tatcctggta gtagccgaga ctcagcttgt tcaccttggc
     3481 cgccatgcgg aacggcgtgt ccggatcata gaggggaccg ctgccgacct gctcaaagcg
     3541 ggcgaacgcc gtatgcacac cagccaggcg cacgctggtt tcggcaagat agccgttcag
     3601 ggaggtgccg ccgcgcggtg cattccttcc ccaggcaagc gtggtcaacc actcgccccc
     3661 ggtcaggttg cgggtatagg tcgctgacag gctggtgcgc cggatttgcc cctcggccac
     3721 cagctggtcg atgcggctcc aggtcccgcg gctgaactgc agcaccaatc ccggcagcgg
     3781 ttggtaggaa aggcgggcgc ggcgggaagt gaagcgcggc tcctcccgca tcgcgtccgg
     3841 ctcgcggggg accgtcgccg ttccttcgac g
//
LOCUS       DATLGF010000503         5113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607 k141_15565,
            whole genome shotgun sequence.
ACCESSION   DATLGF010000503 DATLGF010000000
VERSION     DATLGF010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15565"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VM406_15670"
     CDS             <1..1062
                     /locus_tag="VM406_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HVL77451.1"
                     /translation="AVDVCGLTMLPPDVNLSDYRFTPVGEPGKKATRIRYGLGAVKGS
                     GQNAIEAIIAARESGGPFQDLFDFVKRVDKKQINRRTIEALIRAGAMDCFGVDRGVLM
                     ASVAYAMECAEQAQASANQVSLFGGDDSDLEPPPEYAKVPPWSEKQKLTEEKQALGFY
                     LSGHLFDAYATEVRRFARTKLSSLEPSREPRLLAGIISAVRTQMTQRGKMVIVTLDDG
                     TAVVEVTVYNELYDPNRNLFKEDEFLAVHGKVSEDRFSGGLRVSAEKVMDIAAARVAF
                     AKALRLSMNGQADARRLRELLEPFQQTGTGCPIVLQYTKNGALGELRLSDDWRVRADD
                     ALRAKLGEWLSAENVWFEY"
     gene            complement(1234..2475)
                     /locus_tag="VM406_15675"
     CDS             complement(1234..2475)
                     /locus_tag="VM406_15675"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HVL77452.1"
                     /translation="MHLHSFLATPIPVLDRFAKVCLVFAIFFFPLGHAPAYVFIGLTI
                     LAWLAAGGYIERAQALRGSAFAWAALILYGLFWAGLAYTNADTYYIGIELRKYAKLLF
                     MLVAITLLQEDRWRKRALAAFALAMLITLILSMLSIVWHIPGLKAEVGNRVVFKDHII
                     QNLMMAFFVLIMLVASRTAERTSIRLACLALAVLATVNILGFVAGRTGYVALFAILAV
                     FIACYLPARQRWFFALLALLLGLAAFQFSDTFRQRVETAFSEYRQRNTVEATSVGARL
                     DMIEKSIHLIQERPVMGWGTGAYPEQYCRLQPSQEMCAIGGPHPHNQFLAFGVQFGMV
                     GIFAYLAFLASAVQRSRTYPVPQKVLAWGLIAALLVDSLLHAPLFLVGEALFFTLMLA
                     VVLAPEPRSRAIPAGVPIRQS"
     gene            complement(2534..3412)
                     /locus_tag="VM406_15680"
     CDS             complement(2534..3412)
                     /locus_tag="VM406_15680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVL77453.1"
                     /translation="MKHPPRASDVTVVIVTYNSAHCIAGLAPGLASMPHVTVVDNASN
                     DDTVAEVERLIPQAKLVRNPRNLGFGAANNLALREVTTPFALLLNPDCLPEHAALAGL
                     LETAQRFPEAAIIAPHLLGRSGKLELNYRWSGLHWTSRGPAAEGPCCVGFACGAVMLF
                     NMEVMREVGLFDEDFFLYYEDEDLCHRVLAARRQIIVAPHVTVQHLSRGSVRGNSPLR
                     AEFLRGFHHAQSKLIFTAKHRNAGRTARLRWKTLALALATLPPRLLVPAPRHLARLLG
                     RIWGLCVFKRAPSRNA"
     gene            3679..4383
                     /locus_tag="VM406_15685"
     CDS             3679..4383
                     /locus_tag="VM406_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVL77454.1"
                     /translation="MKWGTKYGPEYVNRLYGMVRRHLSGEFRFVCLTDRSEGIRPEVT
                     CLPIPDLRLPDGLPERGWKKLTTFEADLHGLQGTALFLDLDVVICDSIDCFFDYPGEF
                     LIIHDWKRPWRVTGNSSVYRFRIGAHADVLSQFRSTQAEVRKRFRNEQAYLSDVLHKQ
                     GKLAYWPRDWCSSYKYHCIPTWPTNYWRAPSIPAGTRVIIFHGEVNPPDALEGRRNRA
                     LRFVRRAPWIADFWRE"
     gene            4435..>5113
                     /locus_tag="VM406_15690"
     CDS             4435..>5113
                     /locus_tag="VM406_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HVL77455.1"
                     /translation="MVAPYKGRLAIALLGMILTAATEPMLVAMMKVLLDHGFAADRPF
                     PLWLVPVGLIGVFVLRGVSTFTTTYMMTWVSTRLLNLLRQAIFNRMLDVPLGFNATHP
                     VGKVINAMMFEVQQIVDMIKDVFTSIIRDTLTVIGLMAYLLYLNWRLTLITLVLLPLI
                     ALIVRATGKRLRRLNTTYLGINAELTQVIEETTRAQQVIKIFGGQHYERARFAERAER
                     LRGYAMRM"
BASE COUNT          940 a         1586 c         1688 g          899 t
ORIGIN      
        1 gcggtagacg tgtgcgggct gacgatgctg ccgccggacg tgaacctgtc cgactaccgc
       61 ttcacgccgg tcggcgagcc cggcaagaag gccacccgca tccgctacgg gctgggcgcg
      121 gtgaagggct cgggccagaa cgcgatcgag gccatcatcg ccgcgcgcga atccggcggt
      181 ccgttccagg acctgttcga tttcgtcaag cgcgtggaca agaagcagat caaccggcgc
      241 acgatcgaag cgctgatccg ggccggcgcg atggattgct tcggcgtgga ccgtggcgtg
      301 ctgatggcga gcgtcgccta tgcaatggaa tgcgcggagc aggcccaggc atcggccaac
      361 caggtcagcc tgttcggcgg cgacgattcc gacctcgagc cgccgcccga gtatgcgaag
      421 gtgccgccat ggagcgagaa gcagaagctc accgaggaga agcaggcgct gggcttctat
      481 ctttccggcc acctgttcga cgcctacgcg accgaagtgc gccgcttcgc ccgcaccaag
      541 ctctccagcc tggagccgtc gcgcgagccg cgcctgctcg cgggcatcat cagcgcggta
      601 cgcacgcaga tgacccagcg cggcaagatg gtgatcgtca cgctcgacga cggcactgca
      661 gtcgtggaag tgacggtcta caacgaactc tatgatccga accgtaacct gttcaaggag
      721 gacgagttcc tcgcggtgca cggcaaggtg tcggaggacc gtttctccgg cggcctgcgc
      781 gtatcggccg agaaggtgat ggacatcgcc gccgcgcgcg tggcattcgc aaaggcgctg
      841 cggctgtcga tgaacggcca ggccgacgcg cgccgcctgc gcgaactgct cgagcctttc
      901 cagcagaccg gcaccggttg ccctatcgtg ctgcagtaca cgaagaacgg cgcgctcggg
      961 gagttgcggc tgtcggacga ctggcgcgtg cgcgcggacg atgcgctgcg tgcgaagctg
     1021 ggggagtggc tgagcgcgga gaatgtgtgg ttcgagtatt gatgcgccgt tgtgcgtatc
     1081 ggctccgccg gtcaggaacg cctttcggtc tttagtactg gtgcgggcgt tcgaactggg
     1141 ttcccgcgtg cgcgggaacg acatggcacg agaaagcgct taactgaacg gcattcctcc
     1201 gccgggaggc tgaagctctt ctgttcgccg tacttacgat tgtcggatcg gcacgcctgc
     1261 cggaatggcg cggctgcgcg gttctggtgc gaggacgacg gccagcatca gcgtgaagaa
     1321 cagagcctcg ccgaccagga acagcggcgc atgcagcaat gagtcgacca gcagtgccgc
     1381 gatcaagccc catgcaagca ctttctgcgg gaccggataa gtccgcgacc gctgtactgc
     1441 gctggccagg aaggcgagat aggcgaaaat gccgaccatg ccgaactgca ccccgaacgc
     1501 gaggaattga ttgtgcggat gcgggccgcc gatcgcacac atttcctgcg acggctggag
     1561 ccggcagtat tgttccggat atgcgcccgt tccccagccc atgaccggac gttcctggat
     1621 caggtggatc gacttctcga tcatatcgag gcgggcgccg accgaggtcg cctcgacggt
     1681 attgcgctgc cgatactcgc tgaacgctgt ttccacgcgc tgcctgaacg tatccgagaa
     1741 ctgaaatgcg gccaagccca gtagcagcgc gagcagggcg aagaaccagc gctggcgcgc
     1801 cggcaaatag caggcgatga ataccgcgag aattgcgaac agtgcgacgt atccggttct
     1861 gcctgcgacg aatcccaaga tgttgacggt agcgagtacc gccaatgcca ggcacgcgag
     1921 ccggatactc gtcctttctg cggtgcggct cgccacgagc atgatcagga cgaagaacgc
     1981 catcatcagg ttctggatga tgtggtcctt gaacacgacg cggttgccga cctctgcctt
     2041 caggccggga atatgccaga cgatgctgag catcgacagt atgagtgtga tcaacatggc
     2101 cagtgcgaaa gcggccagtg cacgcttgcg ccacctgtcc tcctgcagca gcgtgatcgc
     2161 caccagcatg aacagcagct tcgcgtattt ccgcagctcg atcccgatgt agtacgtgtc
     2221 ggcgttggtg tatgcgagcc ccgcccagaa cagtccgtac aggatgagcg cggcccaggc
     2281 gaacgcgctt ccccggaggg cttgagcgcg ttctatgtat ccccccgctg ccagccatgc
     2341 aagtatggtc agaccgataa agacataggc aggggcgtgc cccagcggga aaaagaagat
     2401 tgcgaagacc aggcagacct tggcgaaccg gtcgagaaca ggaattggtg tggcgaggaa
     2461 ggaatgcagg tgcaacgtag tctttcggaa ttcaattgcg cgttcacgcg cggggtctgc
     2521 aagattcggg cattcaggcg ttacgggaag gtgcgcgctt gaacacgcac aggccccaga
     2581 tgcggccgag cagtcgcgcg agatggcgcg gcgccggcac cagcagacgc ggtggcaggg
     2641 ttgccagcgc cagcgctagc gttttccacc gcaggcgcgc ggtgcggccc gcgttgcgat
     2701 gcttggcagt gaagatcagc ttcgactgcg catgatgaaa gccgcgcagg aattccgcgc
     2761 gcagcgggct gttgccgcgc accgagccgc gcgacaggtg ctgcacggtg acatgcggcg
     2821 cgacgatgat ctgcctgcgc gcggccagca cccgatgaca caggtcctcg tcctcgtagt
     2881 acaggaagaa atcctcgtcg aacaggccga cctcgcgcat gacttccatg ttgaacaaca
     2941 tcactgcgcc gcaggcgaag ccgacgcagc acggtccttc tgcggccggg ccgcgcgagg
     3001 tccagtgcag gccggaccag cggtagttga gctcgagctt gcccgagcgg ccgagcaggt
     3061 ggggggcgat gatcgccgcc tcggggaacc gttgcgccgt ctcgagcaga ccggcgagcg
     3121 cggcatgctc cggcaggcaa tcgggattca gcagcagtgc gaacggcgtt gtcacctcgc
     3181 gcagcgccag gttgttggcg gcgccaaagc cgaggttgcg tggattgcgg acgagttttg
     3241 cttgggggat caggcgctcg acttccgcga cggtgtcgtc gttgctggcg ttgtcgacca
     3301 cggtcacatg cggcatcgag gccagcccgg gagcgagtcc tgcaatgcaa tgcgcgctgt
     3361 tgtaagtcac gatgacgacg gtgacgtcgc tcgcccgggg cgggtgtttc atgtcggtgg
     3421 cgatgttctt cctgcaagaa cccgtcgcga tggggtatga gtggcgggga attctatcat
     3481 cgcttggcgt gcgctcgcgg cccgccagag ctggcggcgc gttgcgcaag gccgggcaga
     3541 cggggataat cctcaggttt tggcctggcc agccggcgcc gcgcaagcaa cgattgccga
     3601 ggcgacaaga tttgaccaca cggagcagat ttgacgacat ccagcaccgc cggcatcggc
     3661 gaccgcatca tcctctgcat gaaatggggc accaagtacg gtcccgagta cgtcaaccgc
     3721 ctgtacggga tggtgcggcg ccatctgagc ggggagttcc gcttcgtctg cctgaccgac
     3781 cgcagcgagg gcatccgccc ggaggtcacc tgcctgccga ttcccgacct gagactgccc
     3841 gacgggcttc ccgagcgcgg ctggaagaag ctcaccacct tcgaggccga cctgcacggc
     3901 ctgcagggca cggcgctgtt cctggatctg gacgtcgtca tctgcgacag catcgattgc
     3961 ttcttcgact atccgggcga attcctgatc atccacgact ggaagcgtcc ctggcgtgtt
     4021 accggcaatt cctctgtgta tcgtttccgg atcggcgcgc atgctgacgt gctttcgcag
     4081 ttccgctcga cccaggccga ggtgcgcaag cgattccgca acgagcaggc ctacctgtct
     4141 gacgtgctgc acaagcaggg caagctcgcc tactggccgc gcgactggtg cagcagctac
     4201 aagtaccact gcattccgac atggccgacg aattactggc gcgccccatc gattccggcc
     4261 ggcacgcgcg tcatcatctt ccacggcgag gtgaatccgc cggatgcgct ggaaggccgg
     4321 cgcaaccgcg cgttgcgctt cgtgcgccgc gcgccgtgga tcgccgattt ctggcgcgag
     4381 tgacggtgcg ctaacgtgaa gaaactgtct ccccacttgt cacgcctgtt cgcaatggtc
     4441 gcgccgtaca aggggcggtt ggctatcgca ctgctcggca tgatcctgac ggccgcgacc
     4501 gagccgatgc tggtcgcgat gatgaaggtt ctgctcgatc acggcttcgc ggccgaccgg
     4561 ccgttcccgc tctggctggt gccggtcggc ctgatcggcg tgttcgtgct gcgcggcgtg
     4621 tcgaccttca ccaccactta catgatgacc tgggtatcga cccggctgct caatctgctt
     4681 cgccaggcga tcttcaaccg catgctcgac gtgcccctgg gtttcaacgc cacccatcca
     4741 gtcggcaagg tcatcaatgc gatgatgttc gaggtgcagc agatcgtcga catgatcaag
     4801 gatgtgttca cctcgatcat tcgcgacacg ctgaccgtga ttggcctgat ggcctacctg
     4861 ctttacctga actggcggct cacgctgatc acgctggtgc tgctgccgct gatcgcgctg
     4921 atcgtgcgcg cgaccggcaa gcggctgcgc cggctcaata cgacctatct cggcatcaac
     4981 gccgaactga cccaggtgat cgaggaaacc acccgcgccc agcaggtgat caagatcttc
     5041 ggcggccagc attacgagag ggcacgcttc gccgagcgcg ccgagcgcct gcgcggctat
     5101 gcgatgcgca tga
//
LOCUS       DATLGF010000504         2954 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_219099, whole genome shotgun sequence.
ACCESSION   DATLGF010000504 DATLGF010000000
VERSION     DATLGF010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219099"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VM406_15695"
     CDS             <1..675
                     /locus_tag="VM406_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007736321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HVL77456.1"
                     /translation="ERATLTNMTAELGGFTGIVAPDAETVRFLKERRGIDFSLEEWMR
                     SDPGARYAGEICIDCTALSPMVAAPGDPGNGIPLAELGQRVKLDIAYGGSCTAGKRED
                     FDHYHEVLGWAAARGLRVPEGVRLYLQFGTTAVRDYCIEKGYLRAFELVGAELLQPAC
                     GACANCGPGSSTDSGQVTVSAINRNFPGRSGPGQVWLASPPTVAASAIAGELVSFAEL
                     RASHSH"
     gene            706..1689
                     /locus_tag="VM406_15700"
     CDS             706..1689
                     /locus_tag="VM406_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVL77457.1"
                     /translation="MNIRSLARCRVAGLLALALALPALPAAAQLGEKPVRMIVPLTAG
                     STVDIVARLMGNSLSTSLGSPVIVENLVGAGGVTGTAQLVKAPKDGMTLAMVSSNHVI
                     NPSIYKSVPFDSLGDITPISVIGTVPLVLVAHPSVPAQNLKELVALVKAKPGKINYGS
                     AGNGSVLHLAGELLKSEAGGLDMMHVPYRGTGPLVTDLMGGQVDIGFVSVTAAASQIR
                     SGRLRAIGVSTRTRSAILPDVPTLAEQGLPNYSFDAWLAVIGPAGLPKETVNRTHAAL
                     MSALKSAEVREGLATQGVALIGTTPDEARQFFKTELDKHAALVKQSGATLD"
     gene            1692..2201
                     /locus_tag="VM406_15705"
     CDS             1692..2201
                     /locus_tag="VM406_15705"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HVL77458.1"
                     /translation="MNGDRFSTADLHDRHGAGGGLQVVSPGLRAFGAMRACAGRIATV
                     SGAEGDPPRLSEVLRTPGQGRVLVAEGGGVAARHALVGEKMAALAIENGWAGIVLDGL
                     VRDVAVLRVLPIAVFALGSIPSRPAWAETVRMRHDLPLRFLDALFEPGHWLYADEDGI
                     VLAPHALGA"
     gene            complement(2453..2767)
                     /locus_tag="VM406_15710"
                     /pseudo
     CDS             complement(2453..2767)
                     /locus_tag="VM406_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748302.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
BASE COUNT          503 a          942 c          968 g          541 t
ORIGIN      
        1 gaacgagcca cgctgaccaa catgaccgcc gagctgggcg gtttcactgg catcgtcgcg
       61 cccgacgccg aaaccgtgcg ctttcttaag gaacgccgcg gcatcgactt cagcctggaa
      121 gagtggatgc gcagcgatcc cggagcgcgt tatgccggcg agatctgcat cgactgcacg
      181 gcgttgtcgc cgatggtcgc cgcgcccggc gatccaggca acggcatccc gctggccgag
      241 ctcggccaac gcgtgaaact ggatatcgcc tacggcggct cgtgcactgc cggcaagcgc
      301 gaggatttcg accattatca cgaggtgctg ggctgggcgg cggctcgcgg tctgcgcgtg
      361 cctgaaggtg tgaggcttta tctgcagttc ggcaccaccg ccgtacgcga ttactgcatc
      421 gaaaaaggct acctgcgggc gttcgagctg gtcggggccg agctccttca acctgcttgc
      481 ggcgcctgcg cgaactgcgg tccgggctcg tccaccgatt cggggcaggt gacggtgagc
      541 gcgatcaacc ggaatttccc cggccgctct ggccctggcc aggtctggct tgccagtccg
      601 cccacggttg cggccagcgc catcgcggga gaactcgttt cgttcgccga gctgcgcgca
      661 agccattccc actgagtttc actaaataac gacaaggaga caaacatgaa catccgatcg
      721 ctcgcccgct gccgcgtcgc cggcttgctt gcgctcgccc tcgcactgcc ggcgctgcct
      781 gcggccgccc aactcggcga aaagccggta cgcatgatcg tgccgctgac ggccggctct
      841 accgttgaca tcgttgcgcg tctgatgggc aacagcctgt cgacttcgct gggcagcccg
      901 gtaatcgtcg agaacctcgt gggggcgggc ggggtgaccg gcaccgcgca gctggtgaag
      961 gcgccgaagg acgggatgac gctggccatg gtgtcgtcca atcacgtgat caatcccagt
     1021 atctacaagt ctgtgccgtt cgactcgctt ggggacatta cgccgatttc ggtgatcggc
     1081 acggtgccgc tggtgctggt cgcccatcct tccgttccgg cgcagaacct gaaggaactg
     1141 gtggcgctgg tgaaggccaa gccgggcaaa atcaactacg gctcggccgg caatggcagc
     1201 gtgctgcacc tggctggcga gctgctgaag agcgaagccg gcggtctcga catgatgcat
     1261 gtgccgtatc gcggtaccgg cccattggtc accgacctga tgggcggcca ggtggacatc
     1321 ggcttcgtct cggtcaccgc ggcggcgtcg cagattcgca gcggacgtct gcgcgcgatt
     1381 ggcgtctcga ccaggacgcg ctcggccatc cttcccgatg tgccgaccct ggccgagcag
     1441 ggcttgccga actacagctt cgacgcatgg ttggccgtca tcggccctgc cggcctgcca
     1501 aaggaaacgg tgaaccggac ccatgcggcg ctgatgtcgg cactcaagag cgccgaggtc
     1561 agggaaggtc ttgccacgca aggcgtcgcg ctgatcggca ccacgccgga tgaagcgcgc
     1621 cagttcttca agacagaact cgacaagcat gccgctctgg tcaagcagtc cggcgccaca
     1681 ttggattgaa cgtgaacggg gaccggttct cgacagccga cctacacgac cgccatggtg
     1741 ctggcggcgg gctgcaggtg gtctccccgg gcctgcgtgc gtttggcgcg atgagagctt
     1801 gcgcgggcag gatcgccacc gtgtccggtg ccgaaggcga tccgccccgc ttgtcggagg
     1861 tactgcgcac cccggggcag gggcgcgtgc tggtggcgga gggcggcggc gtcgcggcgc
     1921 ggcatgcgct ggtcggcgag aagatggcgg ctttggccat cgagaacggc tgggccggca
     1981 tcgtgctgga tggcctggtc cgggatgtgg ccgtcttgcg cgtcttgccg atcgcggtgt
     2041 ttgcgctggg cagtattccc tcccggcccg cctgggcaga aaccgtgcgc atgcgccacg
     2101 acttgccgct gcgcttcctt gatgcgctgt tcgaaccggg ccactggctg tatgccgacg
     2161 aggatgggat cgtattggcg ccgcatgcgc tgggcgcctg acgaattccg gcgcgccgcg
     2221 catcgcacgc ggcgtgccgg aaatccgtta agcctggttc gtcttcttcc ggcaactact
     2281 cagcccgaat ggctgttagg ccaagtagaa gccgaccgca ccggttgcca gcgggactaa
     2341 tccaatccat ccccaaggtt ctaccgcacc atcatcgtcg ccaccaccaa gcccgcgccc
     2401 accccgatcc gcgccgagca gtcgatgccg cctgtggtgt gagaaaaacc aggagtgcat
     2461 taagcggcag cttcaggtag cgcacgtcat tgccttccgg cggcggctcg ccgcgcgcat
     2521 attgacctgt atcgacggca ggatcagcaa cggcatttcc agcgtggcat cgcgttcgtt
     2581 gcgcatgcac acgaaatcct gttcgctcac gccttcgtgc acgtggatgt tgcgcgcgct
     2641 gctcggcgac ggcgctcact cgcgccggat cgcgcccgct cggcgagtag tcattgcaca
     2701 tgtacaggcg cgtatgcggc ggcaggctca gcaatcggtg gatcgagggg tacaagtttg
     2761 ttccggcact cgaccgggga cagagccttc cgtaccgatc gtggcaagcc gttcaaatgc
     2821 gaatgatcgt taacgtcagt tagtccgcac gtgatagcaa gtcaacaatg tcgactccaa
     2881 gcacgttagc tatccgttca ataacggcca gcgaactatt ccgtccgccg gattcaagct
     2941 gtgaaatata tgtt
//
LOCUS       DATLGF010000505         2404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_235727, whole genome shotgun sequence.
ACCESSION   DATLGF010000505 DATLGF010000000
VERSION     DATLGF010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235727"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1807)
                     /locus_tag="VM406_15715"
     CDS             complement(<1..1807)
                     /locus_tag="VM406_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139763.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HVL77459.1"
                     /translation="MTDLSVAQKLTEYKCQNKVRFVTAASLFDGHDASINIMRRILMA
                     NGAEVIHLGHNRSVEEIVTAALQEDAQGIAISSYQGGHVEYFKYMIDLLKQRGGAHIK
                     VFGGGGGVIVPSEIADLHAYGVARIFSPEDGQRLGLTGMILSMIQACDIDLSTYAPTS
                     LEPLRQGEVADRQRPLAQLITALENNKADPALTKELHTAADTITTPVLGITGTGGAGK
                     SSLTDELIRRLRLDQDDALNIAVISIDPSRRKSGGALLGDRIRMNAINPWGGQQRVFM
                     RSLATREAGSEISQALPDVVAACKVAGFDLVIVETSGIGQGDAAIVPHVDVSMYVMTP
                     EFGAASQLEKIDMLDFADFVAINKFDRKGAQDALRDVAKQYQRNRELFSRRPDEMPVY
                     GTQASRFNDDGVTALYQGVLPKLAELGLKTRPGKLAPVNVRHSSGKNAIVPPARTRYL
                     AEIADTVRGYHGKVKKQVALARERQHLREAQRMLSAAGKDASFDELIAERDAALDPSA
                     RKLLAMWPDMQKAYSGDEYVVKIRDKEIRTRLTYKTLSGNVIRKVSLPKYEDHGEILR
                     WLMLENVPGSFPFTAGVFAFKRENEDPTRMFAGEGD"
BASE COUNT          365 a          780 c          789 g          470 t
ORIGIN      
        1 cgtcaccttc gccggcaaac atgcgggtcg gatcctcgtt ttcgcgcttg aatgcgaaca
       61 cgccggcggt gaacgggaac gagcccggca cgttctccag catcagccag cgcaggatct
      121 cgccgtggtc ttcgtacttc ggcagcgata ccttgcggat cacattgccg gagagggtct
      181 tgtaggtcag gcgggtgcgg atttccttgt cgcggatctt caccacgtac tcgtcgccgg
      241 aatacgcctt ctgcatgtcg ggccacatcg ccagcagctt gcgcgcggac gggtcgagcg
      301 ctgcgtcgcg ctcggcgatc agctcatcga acgaggcatc cttgccggcc gcgctcagca
      361 tgcgctgcgc ttcccgcagg tgctggcgct cgcgcgcgag cgcgacctgc ttcttcacct
      421 tgccgtgata gccgcgcacc gtgtcggcaa tctcggccag atagcgggtg cgcgccggcg
      481 gcacgatcgc gttcttgccg gaagaatggc gcacattgac cggcgccagc ttgcccgggc
      541 gcgtcttcag gccgagttcg gccagcttcg gcagcacgcc ctggtacagc gcggtcacgc
      601 cgtcgtcgtt aaagcgcgag gcctgggtgc cgtagaccgg catctcgtcc ggacgcctgg
      661 aaaacagctc gcggttgcgc tggtattgct tggccacgtc gcgcagcgca tcctgcgcgc
      721 ccttgcggtc gaacttgttg atcgcgacga aatcggcgaa atcgagcatg tcgatcttct
      781 cgagctgcga tgccgcaccg aattccggcg tcatcacata catcgatacg tccacatgcg
      841 gcacgatcgc cgcgtcgccc tggccgatgc ccgaggtttc cacgatcacg aggtcgaagc
      901 cggccacctt gcaggcggcg accacgtccg gcagcgcctg ggaaatctcg gagcccgcct
      961 cgcgcgtggc gagcgagcgc atgaacacgc gctgctggcc gccccacggg ttgatcgcgt
     1021 tcatgcggat gcggtcgccg agcagcgcgc cgccggactt gcgccgcgac ggatcgatcg
     1081 agatcaccgc gatgttgagc gcatcgtcct ggtccaggcg caggcggcgg atcagttcgt
     1141 cggtcagcga ggatttgccg gcgccgccgg tcccggtgat gccgagcacc ggtgtcgtga
     1201 tggtatcggc cgccgtgtgc agttccttcg tcagcgctgg atcggccttg ttgttctcca
     1261 gcgcggtgat cagctgggcg agcggacgct ggcgatcggc cacctcgccc tggcgaagcg
     1321 gctcgagcga ggtcggcgca taggtggaaa ggtcgatgtc gcacgcctgg atcatcgaca
     1381 ggatcatgcc ggtcaggccc agcctttgcc cgtcttccgg gctgaagatg cgcgcgacgc
     1441 catacgcgtg caggtcggcg atctcggacg gtacgatcac gccgccgccg ccgccgaaca
     1501 ccttgatgtg cgcaccgccg cgttgcttga gcaggtcgat catgtacttg aagtactcga
     1561 catggccgcc ctggtagctg gagatcgcga tgccctgggc atcctcctgc agcgcggcgg
     1621 tcacgatttc ctcgaccgag cggttgtggc ccagatggat cacttcggcg ccgtttgcca
     1681 tcaggatgcg gcgcatgatg ttgatcgacg cgtcgtggcc gtcgaacagg gaggcggcgg
     1741 tgacgaagcg caccttgttc tggcacttgt attcggtgag cttctgggca acggaaaggt
     1801 cggtcatggt cgtctcgtcc tcgggttctg ggctggttcg cccggatttt gattttgatt
     1861 acgtttacgt taacgtcaat ctaacatagg gatgaagttc acgtgtgtcg tgggggtgac
     1921 aatcgcggcg gcggctgtcc ctgcagcctg tcgtcccttg cacttgtcgt cccgaacgca
     1981 gtgagggacc tgatccgtct gggcggccac ttcggccccc gcggcctcga tgcatccgga
     2041 tgaggtccat cgccttgctc ggaacgacag ggtggtggtt gttccaaggt acttgtcgtc
     2101 ccgaacgcag tgagggcggc ccgcgtcagg gacctcgtcc gtttgcgccg ctccctctgc
     2161 cggcgtggcc gcgaagcagc cggatgaggt tcctcgctat gctcggaaga caagctgggg
     2221 gctaaccgcc tcacgccgcg tgcggccagc ccttctgcgc ggaaaaacgc gccatcagcg
     2281 tctccagctc gcgccggtac tgctgcaccg ccttgtcctt cacgtgcccg aagccgcgca
     2341 ccttctccgg caggctcgcg atctcgaccg ccagcgcatg attctccacg gacaggttct
     2401 gcag
//
LOCUS       DATLGF010000506         6058 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_107745, whole genome shotgun sequence.
ACCESSION   DATLGF010000506 DATLGF010000000
VERSION     DATLGF010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107745"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..1045)
                     /locus_tag="VM406_15720"
     CDS             complement(233..1045)
                     /locus_tag="VM406_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL77460.1"
                     /translation="MNTRSEPATPSSFVTITRRDGPADLEYRWVGLPHGESAHPVVVF
                     LHEGLGSVSMWHDFPQRFCAAHGLTGLVYSRFGYGQSSVRPRDAALAPRYLHEQAHED
                     LPALLRTLEIRRPWLFGHSDGGSIALLYASRFPEHVSGIVVLAPHIFVEENTVAGLRA
                     ARAAFENGTLTQRLARHHRDVDSVFHGWNDAWLSPAFHGWNIEAEVARIRCPVLAVQG
                     ENDEYATLEQIEGIRRLCPQTRLLVLPGCGHIPHRERAEEVIDAAGAFITGG"
     gene            1636..2241
                     /locus_tag="VM406_15725"
     CDS             1636..2241
                     /locus_tag="VM406_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVL77461.1"
                     /translation="MKVYGDIRSGNCYKVKLLMSLLDIAHEWEHVSILDGQTRQPAFS
                     AMNPNAKIPVLEIEPGVFLSESNAILNYLADGTPYLPSDRLQRARVLQWQFFEQYSHE
                     PYIAVARYISKYLGLPEERRAEFLSKQEGGHKALRVMEQQLAQTSFLTGAVYTIADIS
                     LYAYTHVAQEGGFELSGYPAITAWLERVAAHPKHVTMQQFI"
     gene            complement(2343..2669)
                     /locus_tag="VM406_15730"
     CDS             complement(2343..2669)
                     /locus_tag="VM406_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77462.1"
                     /translation="MQPLQVKDEFPGIAQRVEEVTHMCQITSGLPDELRSVLDELAQE
                     TRHAKAMLREEETGNRIIQCIDRLEKLSDRAMQACSQDSSIDQRLQNAVRHAHDALSD
                     LKHRLH"
     gene            2809..3840
                     /locus_tag="VM406_15735"
     CDS             2809..3840
                     /locus_tag="VM406_15735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HVL77463.1"
                     /translation="MEQEYAGEQEEGGAGVLRRSRIETLAIFRALQLGDMLCAVPALR
                     ALRAALPDTRITLIGLPWAEQFASRYRSYIDDFIAFPGHPDLPEQPVQHERLDDFALA
                     VRERHFDLALQLHGSGEISNRIVSTFGAKACAGYKPKGMPGSSLHYLDFEEGGHESLR
                     LLRLVAFLGAPMQGEHLEFPLFAQDDAELRDSGVARTLVERSYFCIHPGARMRDKCWP
                     LARFAEVADRLCDEFRLQAVITGSGQERDLAEALAAQMRNRPVIAAAPISIGAMAALM
                     SRSRLLICNDTGVSHIAAGLRLPSVVIFSKSDMERWAPLDAELHRCVWDPEGQQGAAV
                     LAHARVLLR"
     gene            complement(3848..4462)
                     /locus_tag="VM406_15740"
     CDS             complement(3848..4462)
                     /locus_tag="VM406_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77464.1"
                     /translation="MPVDVPYSSQWSDPTWTRAIVEEQADPCEDPSWTVRGFSNRDEY
                     RFWARRTCGLACLESMLGYWRLPAPDRANLLRSALARKVYEVSEDGTVRGLIYRPFAD
                     WLQEDHGLRVEVLGEVTLGQLLAKIPGEGFGMASVSPRIRYPTEPCASPGGHLVLIIS
                     SNADEVIFHNPSGIPPYQSHARVRRSDFERFFANRGMVISRPRG"
     gene            complement(4524..5042)
                     /locus_tag="VM406_15745"
     CDS             complement(4524..5042)
                     /locus_tag="VM406_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HVL77465.1"
                     /translation="MSALTVDRFDLQLLAELQRDGKATNSALGEKIHLSTSQVSRRVQ
                     RLEEAHVIDHYAAILEPAAIGLDVMAFTEVTLDRQSTTSSEKFEREVANLPQVLECYS
                     LAGAADYMLRIVAPDLASFSDFMTRHLLRMPGITNVKSTITLQKIKQVHALPLEHVMQ
                     PTESRKRIFYSQ"
     gene            5172..5474
                     /locus_tag="VM406_15750"
     CDS             5172..5474
                     /locus_tag="VM406_15750"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HVL77466.1"
                     /translation="MEKFDSARMAEAMKGLPQWRLDEGRGAIARDFSFKDFAEAFAFM
                     SQVALVAEKRDHHPEWSNVYNKVSIVLTTHDAGGLTQRDVDLAAYADAAYARFGKS"
     gene            5489..>6058
                     /locus_tag="VM406_15755"
     CDS             5489..>6058
                     /locus_tag="VM406_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVL77467.1"
                     /translation="MDFQDQTVLVTGAAGNLGQAVARAFAQRGAKLVLVDRRREALER
                     VFGADNESQILAAVDLLDAAQVAGAVDEAVGRFGAIDVVCNIAGGFDMGPAVHETTDE
                     MWNRLFDLNARTVLQVARAAVPKMKERGRGRIVNVAANAAQKGAAGMGAYCASKDVVV
                     RMTESMAAELREHGINVNCVMPSIIDTPEN"
BASE COUNT         1117 a         1899 c         1934 g         1108 t
ORIGIN      
        1 gtacaggggg gtgcgcttca tccggactcc gacgcaacgg ttgcgccaat ttacaggggt
       61 ccgggcaaac cgttgtcggg gagcagggac cgcgccttgg ctaccagatt cgacagaacg
      121 gcagggttgc ggttgcattt gccgtgatga cagatgcgac cgcctgcctt agatcaatgt
      181 gaggcggata tttcccacgg gggcgggtcg gcaaaatgcg cgcgggcttc gctcagccgc
      241 cggtgatgaa cgctccggcg gcatcgatca cttcctcggc ccgctcgcgg tggggaatgt
      301 gcccgcagcc aggcagcacg agcaagcggg tttgcggaca caggcgacgg atcccttcta
      361 tctgttcgag cgttgcgtac tcatcgttct cgccttgcac tgcgaggaca gggcagcgga
      421 tgcgcgcgac ctccgcttcg atgttccacc cgtggaaggc cgggctgagc caggcgtcgt
      481 tccagccgtg aaacacgctg tcgacgtcgc gatggtggcg cgcgagccgt tgcgtcagcg
      541 tgccgttttc gaacgcggcg cgcgcggcac gcaggccggc caccgtattc tcttcgacga
      601 aaatatgcgg ggcgagtacc acgatgccac tgacgtgttc cgggaaccgg gacgcataca
      661 gcagcgcgat cgatccgccg tcgctgtggc cgaacagcca gggccgccgg atctcgagcg
      721 tcctgagaag cgccggcaaa tcctcatgcg cctgctcgtg caggtagcgc ggcgcgagcg
      781 ccgcatcgcg cgggcgtacg ctcgactgtc catagccgaa gcgtgagtag accaggcccg
      841 tcagcccgtg cgcggcgcag aagcgctgcg ggaagtcatg ccacatcgag accgagccca
      901 gtccctcgtg caggaatacc acgaccggat gtgccgactc gccatgcggc aggccgaccc
      961 agcggtattc gaggtcggca gggccgtcgc gccgggtaat ggtgacgaag ctggaaggcg
     1021 ttgcgggttc gctgcgggtg ttcatctgtt agcggttcct gatcatgcgc gccatcgtac
     1081 cgctgcaaaa gggcgagcag gacaaagcgg ctgtcggatt ttgacgtaaa gaaataaaac
     1141 gcgccaaaac gcttactgac tgcaagttta tttggtatgg agccggaaaa cacgcaaaaa
     1201 agctcgttca acatgtacgt ttttgcttgc acctccttac aaatcgtgta aagattcccc
     1261 ccacaaaaaa tccactgaag gatgggaaga aggagacgcg aggcccgcag gtgcaaacct
     1321 tgcgggcctt tttattgggc ctttttattg ccgccgcccg cctgatataa atggcgtgta
     1381 tatcgggtat cacaacaata aagcggacat ccgaaggggc gcccgatata gttacacctg
     1441 tctcctccaa ctcttctgga attggattcg gcccgaaacc gcaaggtctc gggccttttt
     1501 ttttgccttt gcgcgccggc cgacctgccg cttcacatgc gtggcgctga cggcgcgacg
     1561 ccgcgattct catttgcgcg tccgcgcccg ccatggcagc atggcacgca ccttttacgg
     1621 aaaggacggc gcatcatgaa ggtctacggc gatatccgct cgggcaactg ctacaaggtc
     1681 aagctgctga tgtccctgct ggacatcgca cacgaatggg agcacgtcag catcctcgac
     1741 ggccagacgc ggcagcctgc gttttcggca atgaacccga acgcgaagat ccctgtgctg
     1801 gaaatcgagc caggggtatt cctgtccgaa tcgaacgcaa ttctgaacta tctcgccgac
     1861 ggcacgcctt acctgcccag cgaccgcttg cagcgggccc gcgtgctgca gtggcagttc
     1921 ttcgagcagt acagccacga gccctacatt gcggtcgcgc gctacatctc caaatacctg
     1981 ggcctcccgg aggagcgtcg cgccgaattt ctctcgaagc aggaaggcgg ccacaaggcg
     2041 ctgcgtgtga tggaacagca gctcgcgcag acgtccttcc tcaccggcgc cgtttacacg
     2101 attgccgaca tttcgctgta cgcctacacc cacgtggcgc aggagggcgg cttcgaactg
     2161 agcggatatc ccgccatcac cgcatggctt gaacgggtgg ccgcgcaccc gaagcacgtg
     2221 acgatgcagc agttcatcta gagcgaggct gttctcgccc gctgcacgca caagccccgg
     2281 accctggccc aacgggaggc cgatcagcca gccgacaggg ccgggcggcg cggctacacc
     2341 ggtcaatgca gccggtgctt cagatccgaa agcgcatcat gcgcatggcg caccgcattc
     2401 tgcaggcgct gatcgatgct gctgtcctgg ctgcaggcct gcattgctcg atcgctcagc
     2461 ttttccagcc ggtcaatgca ctggatgatg cgattgccgg tttcttcctc gcgcagcatc
     2521 gccttcgcgt gccgggtttc ctgcgccagt tcgtccagca cgctgcgcag ttcatccggc
     2581 agaccgctgg tgatctggca catatgcgtc acttcctcga cgcgctgcgc gatcccggga
     2641 aattcatcct tcacttgcaa tggttgcatc gcttgcctct tcggaaagtc ccacagaccc
     2701 ggttgagctt cacaatctac tgatagttcc ttagcaacga tcaagtgcgc acgctcgccg
     2761 cacccctatc atcgcaagct gacacttttc gacagggcgt gcaacgacat ggagcaggag
     2821 tatgccggcg agcaagaaga gggcggcgcg ggcgtgttgc gccgctcgcg gatcgagacg
     2881 ctcgcaatat tccgcgcgct gcaactgggc gacatgctgt gcgcggtccc ggccctgcgt
     2941 gcactgcgcg cggccctgcc cgacacccgc atcacgctga tcggcctgcc gtgggccgaa
     3001 cagttcgcat cgcgttaccg cagctacatc gacgacttca tcgccttccc cggacatccg
     3061 gacctgccgg aacagccggt tcagcacgag cgtctggacg acttcgcgct ggcggtgcgc
     3121 gagcgccatt tcgaccttgc cctgcagttg cacggcagcg gcgaaatcag caaccgcatc
     3181 gtgagcactt tcggcgcgaa ggcctgcgcc ggatacaagc ccaagggcat gccgggcagc
     3241 agcctgcatt acctcgattt cgaggagggc gggcacgagt cgctgcgcct gttgcggctg
     3301 gtcgccttcc tcggggcgcc gatgcagggc gagcacctgg agtttcccct ctttgcacag
     3361 gatgacgccg agctgcgcga cagcggcgtt gcacgcaccc tggtggagcg cagctacttc
     3421 tgtattcacc caggagcgcg catgcgcgac aagtgctggc cgctggcccg ctttgccgag
     3481 gtggccgacc ggctgtgcga cgaattccgc ctgcaggcgg tcattaccgg ctccggacag
     3541 gagcgcgatc tcgccgaggc gctcgccgcg cagatgcgca accgtccggt gatcgctgcg
     3601 gcgccaatct ccatcggtgc gatggcggcg ctgatgagcc gctcgcgcct gctgatctgc
     3661 aatgacaccg gcgtgtcgca catcgcggcc ggcctgcgcc tgcccagcgt ggtcatcttc
     3721 agcaagtccg acatggagcg ctgggcgccg ctggatgcgg aactgcaccg ctgcgtgtgg
     3781 gacccggaag ggcagcaggg cgcggcggtg ctcgcgcacg cgcgtgtgct gctgcgctag
     3841 cgcgcggtta accgcggggc cgcgaaatga ccatgcctct gttggcgaag aagcgttcga
     3901 aatcgcttcg ccgcactctt gcgtgggact gatacggcgg aatgcctgag ggattgtgga
     3961 agatgacctc gtcggcattc gaggagatga tcagtaccag atgcccgcct ggtgatgcac
     4021 agggctcggt tgggtacctg atgcggggac tgacagaggc catgccgaag ccctcgcccg
     4081 gtattttcgc cagcagttgc cccagtgtga cctcgccaag gacctcgacc cggagaccat
     4141 ggtcttcttg aagccaatcc gcgaacggtc gatagatcag cccgcgtacc gtcccgtctt
     4201 ccgacacctc atacaccttg cgggcaaggg cggatcgaag caggtttgct cggtcaggtg
     4261 ccggcaagcg ccagtagccg agcatggatt ccaggcaggc gagaccgcag gtccgtctcg
     4321 cccagaagcg gtactcgtcc ctgttgctga acccgcggac ggtccagctc ggatcttcgc
     4381 aagggtccgc ctgctcctcg acgatggccc tggtccaggt cgggtccgac cattgggagg
     4441 aatagggaac atccaccggc atcgcgacac ctttgagcta gcaaacgatg ccagcttaac
     4501 agagtagtcc ggcggtcccc tcgctactgc gaatagaaga tccgcttgcg actctcggtc
     4561 ggctgcatca cgtgctcgag cggcagcgcg tgcacctgct tgatcttttg cagcgtaatg
     4621 gtcgacttga cgttggtgat gcccggcatg cgcagcagat ggcgcgtcat gaaatccgaa
     4681 aacgaggcca ggtcgggcgc gacgatgcgc agcatgtagt cggcggcccc tgccagcgag
     4741 tagcattcca gcacctgcgg caggttcgcg acctctcgtt cgaacttctc gctcgaagtc
     4801 gtgctctggc ggtccagcgt gacttcggta aacgccatca cgtccaggcc gatcgccgcc
     4861 ggttcgagta tcgccgcgta atgatcgatc acatgcgctt cctcaagccg ctgcacgcgc
     4921 cggctgacct gcgacgtgga caggtgtatt ttttcgccga gcgcgctatt ggttgccttg
     4981 ccgtcgcgct gcagttcggc aagcagctgc agatcgaagc gatccacggt gagtgcggaa
     5041 atttttccca tgtttggccc aatgtttgca cattttgtgc gcatcctacc actcgccgcg
     5101 gacagaatga gcacaccgtg cgggcgcacg ggaatacact ttttgcaatt gagaacatgg
     5161 aaggagcaac gatggagaag ttcgattcag ccaggatggc cgaggcgatg aaagggctgc
     5221 cgcagtggcg cctcgacgag gggcgcggtg cgatcgcgcg tgatttcagc ttcaaggact
     5281 tcgccgaggc gttcgccttc atgagccagg tcgcgctggt cgcggaaaag cgcgatcacc
     5341 acccggagtg gtcgaacgtc tacaacaagg tttcgatcgt gctgaccacc catgacgccg
     5401 gcggcctgac gcagcgcgac gttgatctcg ccgcctatgc cgacgccgcc tatgcgcgct
     5461 tcggcaagag ctgaaagagg agaacgacgt ggacttccag gaccagacag tgttggtgac
     5521 cggcgcggcc ggcaatctcg gccaggcggt cgcgcgcgcg ttcgcgcagc gcggtgcgaa
     5581 actggtgctt gtggaccggc gccgcgaggc ccttgagcgc gtgttcggtg cggataacga
     5641 aagccagata ctggcggccg tcgacctgct cgatgcggcg caggtggccg gcgcggtgga
     5701 tgaggcggtt ggccgcttcg gcgcgatcga cgtcgtctgc aacatcgccg gcggtttcga
     5761 catgggcccc gccgtgcacg agacgacgga cgagatgtgg aaccgtttgt tcgacttgaa
     5821 tgcccgcacc gtgctgcagg tggcgcgcgc ggccgtgccg aagatgaagg aacgcgggcg
     5881 cggcaggatc gtcaacgtcg ccgccaacgc cgcccagaag ggcgcggccg gcatgggcgc
     5941 gtactgcgcc tccaaggacg tggtcgtgcg catgaccgaa tcgatggcgg ccgagctgcg
     6001 cgagcacggc atcaacgtca actgcgtgat gccgagcatc atcgacacac cggagaac
//
LOCUS       DATLGF010000507         7517 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_203629, whole genome shotgun sequence.
ACCESSION   DATLGF010000507 DATLGF010000000
VERSION     DATLGF010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 7517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7517
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203629"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VM406_15760"
     CDS             <1..540
                     /locus_tag="VM406_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HVL77468.1"
                     /translation="ILAALIGVQASGRGRFVDISMTDTVFAHNVMPLVAVNNFGRPAP
                     PGGDLLTGGVPCYNVYRTSDGRYMAVGALELKFWESCCDVLERPDLKPRHWSRGQRVG
                     GEDAKAVKAELDAIFARRTLAEWTEQFAAADCCVTPILRMDEAIAHPLFQARAMIAQR
                     EHPTEGSYWATAPAVKFQA"
     gene            548..1384
                     /locus_tag="VM406_15765"
     CDS             548..1384
                     /locus_tag="VM406_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HVL77469.1"
                     /translation="MNIPSLNIVGCGRLGRTLGRLWHEAGVFRVQDILTRSLPGACHA
                     AGFIGSGRPVGRMEDLRCADVWLVATGDSEIESCCAHLAAARLFSAGNTVLHCSGALS
                     SEALRAAANAGAATASMHPIKSFADPARTAATFAGTWVGAEGHAEALALLEPAFTRIG
                     ARVVAIEAEHKRIYHAASVFASNYLVTLLDVARQAYIKAGVPPETAMQVMEPLVRGSV
                     DNVFALGTEQALTGPVARGDVATVVSQYRAVKQWDRRCGALYKQLAKLTRDLAARAKH
                     KR"
     gene            complement(1445..1747)
                     /locus_tag="VM406_15770"
     CDS             complement(1445..1747)
                     /locus_tag="VM406_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77470.1"
                     /translation="MRLSILGFFLLSYMLAVPALADSGVRDAVRMSHLQNSAPGQMER
                     RGAREFRIPEVSGNGQLADDAVAEGVRRSGKLSLEERRALRRQIDEAGHDIYSSNR"
     gene            1998..2750
                     /gene="ompR"
                     /locus_tag="VM406_15775"
     CDS             1998..2750
                     /gene="ompR"
                     /locus_tag="VM406_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HVL77471.1"
                     /translation="MNTTNPHSNTPAHHGKILVVDDDLRLRDLLRRYLSEQGFNVVTA
                     ENAQAMNKLWIRERYDLLVLDLMLPGEDGLSICRRLRGANDQTPIIMLTAKGEDVDRI
                     VGLEMGADDYLPKPFNPRELVARINAVLRRKGPDEIPGAPSETPQTFAFGDFVLDLGT
                     RTLRKNGTTISLTTGEFSVLKVFARHARQPLSREKLMELARGREYEVFDRSLDVQISR
                     LRKLIEPDPSNPMFIQTVWGLGYVFIPDGQSR"
     gene            2764..4116
                     /locus_tag="VM406_15780"
     CDS             2764..4116
                     /locus_tag="VM406_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL77472.1"
                     /translation="MTPRIGKRLGWLTSGLFWRTFFLLAFLITASLVTWVTSFRAVER
                     EPRAQQVAAQVISIVTVTRAALTHSAPDLRRELLFDLASNEGIRIYPLEASDVVVPPA
                     PGGIMPMVEADVKARLGAETKFASRVNDVAGFWVSFHIDDDEYWLRLDRERIERWSNI
                     QWLGLASTTLVLSLLGAVFISRLINQPLSRITSATHEIAKGRFPAPLPEAGPTEIREA
                     NRSFNQMVADLNRVEADRAVILAGISHDLRTPLARMQLEVEMANLPEDAREGMRSDLT
                     QMDAIIGQFLEYAKPASNAQLASVDLSGLLHECAREVARLPDVCVTTAIDPDVNVFGN
                     ATDLKRVISNLLENARRYGKAEGTSQAQIELACKTENGRAVIEVADNGPGVPQEDIER
                     LLRPFTRLDAARGQANGAGLGLAIVDRIVKRHHGSLRLANREQGGLLIRVSIPITGAL
                     "
     gene            4230..4433
                     /locus_tag="VM406_15785"
     CDS             4230..4433
                     /locus_tag="VM406_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77473.1"
                     /translation="MPASPPSGGSDKPAAPHLKPGDEAYAGTPGTGEDLCEVCGGTGI
                     RQDGSPCPNCEGTGRVTRGIGGA"
     gene            complement(4434..4928)
                     /gene="ispF"
                     /locus_tag="VM406_15790"
     CDS             complement(4434..4928)
                     /gene="ispF"
                     /locus_tag="VM406_15790"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HVL77474.1"
                     /translation="MSTTTLAFRIGQGYDCHALVPGRKLIVGGVEIPHPHGLLGHSDA
                     DVLLHALTDALLGAAGLGDIGAHFPDTDARFAGADSRVLLRTAAERVGQAGYLVGNVD
                     ATVIAQAPKMAPHIPAMVNNIAADLGIEAAQVNVKAKTNERLGYLGREEGMAAQAVVL
                     LLRR"
     gene            complement(4925..5623)
                     /gene="ispD"
                     /locus_tag="VM406_15795"
     CDS             complement(4925..5623)
                     /gene="ispD"
                     /locus_tag="VM406_15795"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HVL77475.1"
                     /translation="MTASRHFALIPAAGIGARLGAETPKQYLPIAGKPMLLHVLETFA
                     AVEAVDHTWVVVGRDDGYIDALMEASATLATRVTVLRCGGTSRQRSVQNGLAAIRAEV
                     GDDDRILVHDAARPGLTPELVERLIAALRDDPVGGLLALPVVDTLKRSDAQARAEATV
                     PREHLWAAQTPQMFPYALLRRALEQAAGVTDEASAIEALGLRPRLVEGSPRNFKVTLP
                     HDVALAELHLKASR"
     gene            5749..>7517
                     /locus_tag="VM406_15800"
     CDS             5749..>7517
                     /locus_tag="VM406_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HVL77476.1"
                     /translation="MSFDLKKNLPKPGQRFALPSLCGSADAFVLARAAQELKGRGQML
                     TVVTANATDAQRLLDEIAWFAADEENPLRCHLLPDWETLPYDAFSPHQDLVSERLATL
                     HEVSSGACDVLLVPATTALLRMAPPSFLAAYTFFFKQGETLDEAKLRQQLTLAGYSHV
                     AQVMSPGEYSVRGGLIDLFPMGSPLPYRLDLFGDTIETIRTFDADTQRSLYPVREVRL
                     LPGREFPMDEAARTAFRSRWREEFEGDPSKSPIYKDIGNGVPAAGIEYYLPLFFEQTA
                     TLLEYLPDDAAFALIGDVDAAIKRFWSDTQSRHKFLKADRERPLLEPHRLFLTDEDFF
                     SLLKPRARWVMQSGEATSEICAPLPNIAVNRRTEDPLVNLRSFLLQTDKRVLICAESG
                     GRRETLQQYFNEFDLNLPVCEGFADFLTGSARAMLGIAPLHAGFQLDASLGDIAFITE
                     TELYAGTGRRAGRRKQEAVTQVESMVRDLSELKVGDPVVHINHGIGRYMGLVGMDLGE
                     GETEFLHLEYAKETKLYVPVSQLHVISRYSGASPDDAPLHTLGSGQWEKAKRKAAQQV
                     RDTAAELLNLYARRAARQGHVFE"
BASE COUNT         1296 a         2540 c         2433 g         1248 t
ORIGIN      
        1 atcctggcgg ccttgatcgg cgtgcaggcc agcggtcgcg gccgcttcgt cgacatctcg
       61 atgacggaca cggtgttcgc gcacaacgtg atgccgctgg tcgcggtcaa caacttcggg
      121 cgccccgccc cgcccggcgg cgacctgctg accggcggcg tgccctgcta caacgtctac
      181 cgcaccagcg acggccgcta catggccgtc ggcgcgctcg agctgaaatt ctgggaatcc
      241 tgctgcgacg tgcttgagcg tccggacctc aagccacggc attggagccg cgggcagcgc
      301 gtcggcggcg aggatgcaaa ggcggtgaaa gccgagctgg acgcgatctt cgcgcgccgc
      361 acgctggcgg aatggaccga gcagttcgcg gccgccgact gctgcgtgac gcctatcctg
      421 cgcatggacg aagcgatcgc ccatccgctg ttccaagcgc gcgcaatgat cgcgcaacgc
      481 gagcatccga ccgagggcag ctactgggcc accgcgccgg cggtgaagtt ccaggcgtga
      541 cacggcggtg aacattccct cgctcaacat cgtcggctgc ggccgcctgg gccgcacgtt
      601 ggggcgcctc tggcatgaag ccggcgtctt ccgcgtccag gacatcctga cgcgctcgct
      661 ccccggcgcc tgtcacgccg cgggcttcat cggcagcgga cgcccggtgg ggcgcatgga
      721 ggacctgcgc tgtgcggacg tctggctggt tgcgaccggc gacagcgaga tcgagtcatg
      781 ctgcgctcat ctggctgccg caaggctctt ctccgccggc aataccgtgc tccactgcag
      841 cggcgctttg tcgtcggagg cgctgcgcgc cgccgccaac gccggcgcgg ccaccgccag
      901 catgcatccg atcaagagct ttgccgaccc ggcgcgtacc gccgcgacct tcgccggcac
      961 ctgggtcggc gcggagggtc atgccgaggc cctggcactg ctggagccgg ctttcacgcg
     1021 aatcggcgcg cgggtcgtcg cgatcgaggc cgagcacaag cgcatctatc acgccgcatc
     1081 cgtgttcgcc tccaactacc tggtgacttt actcgatgtc gcccggcagg cttacatcaa
     1141 ggccggtgtg ccgccggaga ccgcgatgca ggtgatggaa ccgctggtgc gcggcagcgt
     1201 ggacaacgtg ttcgcgctcg gcaccgagca agcgctgacc ggtccggtcg cgcgcggcga
     1261 tgtcgccact gtcgtcagcc agtaccgggc cgtgaagcaa tgggatcgtc gctgtggtgc
     1321 gctgtacaag cagctggcga agctgacaag ggacctcgcc gcaagggcga agcacaagcg
     1381 ttgaagtgca ttgaagcttc gttgcgccgg cagctgtcgt gggcgggggg cgatcgaacg
     1441 aaaactacct gttgctggag tagatgtcgt gaccggcttc gtcgatctgg cgccgcagcg
     1501 cgcggcgctc ttccagcgac agcttgccgg aacggcgcac cccctcggcg acggcatcat
     1561 cggccagctg accattgccg ctcacttccg ggatccggaa ttcacgtgca ccacgccgct
     1621 ccatttggcc tggcgccgaa ttttgcagat gcgacatgcg cactgcgtcg cgcacccccg
     1681 agtctgccag ggcgggaacg gccagcatgt aggacaacag gaagaaaccg agaatagata
     1741 gtcgcatgct cttatgccga ctaagtcgcc ttggaaggcg ccggacggcg gatcatcggg
     1801 gttgctgcgg gaaaacagca gagagacaac gggaacacga cgcgccgctg cgggacttcc
     1861 gctcgtgaaa cgcgccggat tcaagtgtat gcttcatcct ccgggtgcag aacgcccctg
     1921 acgtaaagtt tgtaacagcc tgtaaaggct tgcgcaacgt ctcttgccgg caagatcgtc
     1981 gacgttacca tagcgccatg aatacgacaa atccacatag caacactccc gcccatcacg
     2041 gcaagatcct ggtcgtcgat gacgacctcc gcctgcgcga cctgctgcgc cgctacctgt
     2101 ccgaacaggg cttcaacgtg gtcacggccg agaacgccca ggccatgaac aagctctgga
     2161 tccgcgagcg atacgatctg ctcgtgctcg atctgatgct gcctggcgaa gacggcctgt
     2221 ccatctgccg ccggctgcgt ggtgcgaacg accagacccc gatcatcatg ctgacagcaa
     2281 aaggcgagga cgttgatcgc atcgtcggcc tggagatggg tgccgacgac tacctcccaa
     2341 agccattcaa tccacgcgaa ctggtggcgc gcatcaacgc ggtgctgcgt cgcaagggac
     2401 cggacgagat tcctggcgcg ccgtcggaaa cgccgcagac cttcgcgttc ggcgacttcg
     2461 tgctcgacct tggcacgcgc acgctgcgca agaacggcac cacgatctcg ctgaccaccg
     2521 gcgaattctc cgtgctcaag gtctttgccc gccatgcgcg ccagccgctg tcgcgcgaga
     2581 aattgatgga actggcgcgc ggccgcgagt atgaagtgtt cgaccgcagt ctcgatgtcc
     2641 agatctcgcg cctgcgcaag ctgatcgaac cggacccgtc caacccgatg ttcatccaga
     2701 cggtatgggg tctgggctat gttttcattc cagacggcca gtcgcgctga ctccgcgccc
     2761 accttgactc cccgcatcgg caagcgcctg ggctggctca cgagcggcct gttctggcgc
     2821 acgttcttcc tgcttgcctt cctgatcacc gcaagcctcg tcacctgggt gacgagcttt
     2881 cgggcagtcg agcgcgaacc ccgcgcacag caggtggcgg cccaggtcat ctccatcgtc
     2941 acggtcaccc gcgccgcgct gacccactcg gcgcctgact tgcgccgcga actgctgttc
     3001 gacctggcca gcaacgaagg catccgcatc tacccgcttg aagcgagcga cgtggtcgtg
     3061 ccgcccgcgc ccggcggcat catgccgatg gtggaggcgg atgtgaaggc acggctcggg
     3121 gcggaaacca agttcgcctc gcgagtcaac gacgtggccg gtttctgggt cagtttccat
     3181 atcgatgatg acgagtactg gctgcgcctg gaccgcgagc gtatcgagcg ctggtccaat
     3241 atccagtggc tcggccttgc cagcaccacg ctggtgctgt ccttgctcgg ggccgtgttc
     3301 atttcgcggc tgatcaacca gccgctgtcg cgcatcacct cagccaccca tgaaattgcc
     3361 aagggccgct ttcccgcgcc gctgcccgaa gcaggcccga cggagatccg cgaggccaac
     3421 cgcagcttca accagatggt ggccgacctc aatcgcgtcg aagctgaccg cgccgtgatc
     3481 ctggccggca tctcgcacga cctgcgtacc ccgctggcac gcatgcagct cgaagtcgag
     3541 atggcaaacc ttccggaaga cgcgcgcgag ggcatgcgct cggacctgac ccagatggac
     3601 gcgatcatcg gacagttcct tgagtatgcg aagccggcga gcaatgcaca gcttgccagc
     3661 gtggacctga gcgggctgct gcacgagtgc gcgcgcgaag tggcgcgcct gccggatgtt
     3721 tgcgtgacga ccgccatcga tcctgacgtg aacgtgttcg gcaacgcgac ggatctcaag
     3781 cgcgtgatca gcaacctgct ggaaaacgcg cgccgctacg gcaaggcgga gggcaccagc
     3841 caggcccaga tcgagcttgc ctgcaaaacc gagaacggcc gcgcggtcat tgaagtggcg
     3901 gacaacggcc ccggtgtgcc ccaggaagac atcgaacgct tgctgcggcc gttcacccgg
     3961 ctcgacgccg cccgcggaca ggcgaacggc gcaggcctgg gattggcgat cgtcgaccgt
     4021 atcgtcaagc gccatcatgg cagcctgcgc ctggccaatc gagaacaagg cggacttctg
     4081 atccgggtct cgatcccgat taccggcgcg ctctgacccg gcccggcagc aggcgcgcgt
     4141 cttgccggaa gcaaataccg gttcccccct gaacttcgct gcgccgatcc aatctatcgg
     4201 ccattgccat ttcgttcaca ggaggtccca tgccagcctc tccaccatcc gggggcagcg
     4261 acaagccggc ggcaccgcac ctgaaaccgg gcgatgaagc ctatgcgggg acgccgggca
     4321 ccggcgagga tctgtgcgaa gtctgcggcg ggaccggaat ccgccaggac ggcagccctt
     4381 gtccgaactg cgagggaacc ggccgcgtga cgcgcggcat cggcggcgcc tgatcagcgg
     4441 cgcagcagca gcaccacggc ctgcgccgcc atgccctcct cgcgccccag atagcctagc
     4501 cgttcattgg tcttggcttt cacattgacc tgtgcggcct cgattccgag gtcggcggcg
     4561 atgttgttca ccatcgccgg aatatgcggt gccatcttcg gcgcctgcgc gatcactgtt
     4621 gcatcgacat tgccgaccag atagccggct tgccccaccc gctcggccgc cgtgcgcaac
     4681 agcacgcgcg aatcggcgcc ggcgaagcgg gcatcggtgt ctgggaaatg ggcgccgatg
     4741 tctcccagtc cggcggcgcc cagcagcgcg tcggtcagcg cgtgcagcag tacgtcggca
     4801 tcggaatgac cgagcaggcc gtgagggtgc gggatttcga cgccgccgac gatcagcttg
     4861 cgcccgggaa cgagggcgtg gcagtcgtag ccctgcccta tgcgaaaagc gagtgtcgtt
     4921 gtgctcatcg ggaagctttc agatggagtt cggccagcgc gacatcgtgc ggcagcgtga
     4981 ccttgaagtt gcgcgggctg ccctcgacca gccgcggcct gagtccgagc gcctcgatcg
     5041 cgctggcctc gtcggtgacc cctgccgcct gttccagcgc gcggcgcagc agcgcatacg
     5101 ggaacatctg cggcgtctgc gcggcccaca ggtgctcgcg cggcacggtc gcctccgcgc
     5161 gcgcctgggc atcgctgcgc ttcaaggtgt cgaccaccgg cagtgcgagc agtccgccga
     5221 ccggatcgtc gcgcagcgcc gcgatcagac gctcgacgag ttctggcgtg aggcccggac
     5281 gggcggcatc gtgcaccagg atgcgatcgt cgtcgccgac ctcggcgcgg attgccgcca
     5341 gcccgttctg cacggaccgc tgccggctcg tgccgccgca gcgcagcacg gtcaccctcg
     5401 tcgcaagcgt ggcggacgcc tccatcagcg cgtcgatgta gccatcgtca cggccgacga
     5461 cgacccaggt atggtcgacg gcttcgaccg cggcgaaggt ctcgagcaca tgcagcagca
     5521 tcggcttgcc ggcaatcggg aggtattgtt tcggggtttc agcgccgagg cgagcgccga
     5581 tgccggcggc gggaatcagg gcgaaatggc gggatgcggt catcgggagg caggcggggg
     5641 ggcggaaatc aggaagccgc ccgtatataa tcgggggtta ttttctcaca gtcgaccgcg
     5701 cggattttcc cctgttccgc cgcctcccgg catgcgagtg cccgtctgat gtccttcgat
     5761 ctcaagaaaa atcttcccaa acccggtcag cgtttcgcac tgccgtccct gtgcggttcg
     5821 gcggacgctt tcgtgctcgc gcgcgccgcg caggaactga aaggccgcgg acagatgctg
     5881 accgtcgtca ccgcgaacgc gaccgacgcc cagcgcctgc tcgacgagat cgcatggttc
     5941 gcggccgacg aggaaaaccc gctgcgctgc catctgctgc cggactggga aaccctgccc
     6001 tacgatgcgt tctcgccgca ccaggacctg gtatccgagc gcctcgccac cctgcatgaa
     6061 gtcagcagcg gcgcctgcga cgtgctgctg gtgccggcca ccaccgcgct gctgcgcatg
     6121 gcgccgccgt cgttcctggc cgcctacact tttttcttca agcagggcga gacgctcgac
     6181 gaggcgaaac tcaggcagca gctgacactg gcaggctaca gccatgtcgc ccaggtgatg
     6241 tcgccgggcg agtattcagt gcggggcggc ctgatcgacc tcttcccgat gggttcgccc
     6301 ctgccctacc ggctcgacct gttcggcgac accatcgaga ccatccgcac cttcgacgcc
     6361 gacacccagc gctcgctgta cccggtgcgc gaggtacgcc ttttgccggg ccgcgaattc
     6421 ccgatggacg aagcggcgcg caccgcgttt cgcagccgct ggcgcgagga gttcgagggc
     6481 gatccgagca agtcgccgat ctacaaggac atcggcaacg gcgtgccggc ggccggcatc
     6541 gagtactacc tgccgctgtt cttcgagcag actgcaaccc tgctcgagta cctgccggac
     6601 gacgccgcct tcgcgctgat cggcgacgtc gatgccgcga tcaagcgctt ctggagcgat
     6661 acccagtcgc gccataaatt cctgaaagcc gaccgcgaac gcccgctgct cgagccgcat
     6721 cgcctgttcc tgaccgacga ggacttcttc agcttgctga agccgcgtgc gcgctgggtg
     6781 atgcaatcgg gcgaagcaac atcggagatc tgcgctccgc tgccgaacat cgcggtgaac
     6841 cggcgcaccg aggatccgct cgtcaacctg cgttccttcc tgctgcagac ggacaagcgc
     6901 gtgctgatct gcgccgagtc gggcggacgg cgcgagacgc tgcagcaata cttcaacgaa
     6961 ttcgacctca accttccggt ctgcgaaggc tttgccgatt tcctcaccgg cagcgccagg
     7021 gccatgctcg ggatcgcgcc gctgcatgcg ggcttccagc ttgatgcatc gctcggcgat
     7081 atcgccttca tcaccgaaac cgagctgtat gccggcaccg gacgccgggc ggggcggcgc
     7141 aagcaggaag cggtgaccca ggtcgaatcg atggtgcgcg acctgtccga gctgaaggtc
     7201 ggtgatcctg tcgtgcacat caaccacggc atcggccgct acatgggcct ggtcggcatg
     7261 gacctgggcg aaggcgagac cgagttcctg cacctggaat acgcgaagga gaccaagctg
     7321 tatgtgccgg tctcgcagct gcacgtgatt tcccgctatt ccggcgcctc gccagacgac
     7381 gcgccgctgc atacgctcgg ctcgggccag tgggagaagg cgaagaggaa ggcagcccag
     7441 caggtgcgcg acaccgcagc cgaactgctg aacctgtatg cgcgccgtgc cgcgcgccag
     7501 ggccatgtgt tcgagta
//
LOCUS       DATLGF010000508         9289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_107855, whole genome shotgun sequence.
ACCESSION   DATLGF010000508 DATLGF010000000
VERSION     DATLGF010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 9289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9289
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107855"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VM406_15805"
     CDS             complement(<1..286)
                     /locus_tag="VM406_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HVL77477.1"
                     /translation="MKNDTPALVKSALQDVFERQHAASRAHAPLPLARRLEALDTLLN
                     GVLQWEQRLIDALHADFGNRSSHETQLLELFPLVDEIRHIKRNLRRWMRPR"
     gene            complement(283..1941)
                     /locus_tag="VM406_15810"
     CDS             complement(283..1941)
                     /locus_tag="VM406_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HVL77478.1"
                     /translation="MHYDYDYDYDFVVIGSGFGGSVSALRLVEKDYRVAVVEQGKRWT
                     ADNMPKNNWDVARWVWRPALGMRGFFSMQFFRHVVVLHGNAVGGGSIVYANTLLVPPD
                     TVWRQGSWAGLNDWERVMPAHYATARRMLGVTTNRRLGPADLKLREMAALVGAEDSFY
                     PTEVGIFFGKDEDPPGSAYADPYFGGEGPARNSCTACGGCMVGCRHNAKNSLDKNYLY
                     LAEKKGAALLAETRVVDVRPLAAREDGSAGYEVQTVSLAGDTRGAKRRLTCRGVVFAA
                     SSLGTQELLFRLKEKRSLPRVSDALGRHVRTNAESLIGIRFPGTRTDLSEGVAIGSGV
                     YIDEHTHIEAVRYPKGSDSMGMLSTVMTLGRPGPTRILTWLATLARLLVTRPISTLRV
                     LSPFGSARESIIFLCMQTLDGHLTMRLKRRWFWPFSKQLATHGERIPTFIPAANDFAM
                     KAAQATGGVAQTSITEILLNIPMTAHCMGGAAMAASPADGVCDGKNRVFGYHNMYICD
                     GSMLGANLGVNPSLTITALTEHAMSHVPPKSAQRWDATGTEVPA"
     gene            complement(2129..3376)
                     /locus_tag="VM406_15815"
     CDS             complement(2129..3376)
                     /locus_tag="VM406_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HVL77479.1"
                     /translation="MQTRRYPLAAILLLALLLSGCSRAPVSGQLVDFVSGEPIEGAHV
                     TLAQRGWGVSGAQLVWDRSYAASARTQADGRFTLSLPAPLLAGRRARLEVAHNGYQRL
                     TEVAAHAGAALRLQAMPVPPQSVPGGLATFGLLADGQPFGWSFVENRPTADPALADLF
                     PEHVRRAPLGLALAAPGGGVQLLTQQAQGIATASYGHLLRYAARAPDSGYRRHVELDG
                     NTTATLFVRTRSDRYAKLAFDPQRLSSIQGKVDGLDQPVAYAVQLPFAYNPWPGRDLP
                     FDPVSAVRSVDPLLAAAAAELPVDGKPSAAARHIELRTVDERGNPVDRMSMLLEPDLP
                     RAFECPDARFQYRDVVLGYGSDGLPRVRLSVHGAGFVHHHAPVLVSRRLPAMLRFEQY
                     GSAPRPVSYVLQLREVDGPATGC"
     gene            3708..4415
                     /locus_tag="VM406_15820"
     CDS             3708..4415
                     /locus_tag="VM406_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77480.1"
                     /translation="MARRKSLSQAPLRADQPSAETAASTGSQEGAASRASSIRVAIVL
                     SALVGGVFCVLADLMQKSDASAVEHLGRKLGETFSLVQGATLLATLTVLVLAVAVPLI
                     FESSTKTSAFYLGASVLAILMTMTPYEPPSGFQTLPNSVEVLLSIETADGKPANGARV
                     TLRDAGGTQIISRSRLPKSDLRFFQDEGVYRLTVELAGYEVATERLVLKEGQPPQTMK
                     VKLQPSDKPRILQRILQ"
     gene            complement(4438..4890)
                     /locus_tag="VM406_15825"
     CDS             complement(4438..4890)
                     /locus_tag="VM406_15825"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF014590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HVL77481.1"
                     /translation="MGKRGESWVAAQAILFIAFITVPQVAGPWPYDAVFLFTGWVLFV
                     LGLVLTLWSARALGRSLTPFPRPLEHAELVTTGPYRLVRHPIYLGVLLVALGISLVTA
                     SPLRLGLTLILFVFFDRKAAREERWLEEKFPQYPAYRSRVKRLIPWVY"
     gene            4907..5059
                     /locus_tag="VM406_15830"
     CDS             4907..5059
                     /locus_tag="VM406_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77482.1"
                     /translation="MHLSVNDRQMDAEVPASRMRGAGCAELFQIRHTFRHFEAALLAF
                     SQREIE"
     gene            5175..6302
                     /locus_tag="VM406_15835"
     CDS             5175..6302
                     /locus_tag="VM406_15835"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL77483.1"
                     /translation="MNIKHVFVACIAAGMAAGACADEIRIGIVSGMTGPIAETAEQIA
                     AVSRGYVDMINARGGINGSRLALVIKDDQYDPRKTAPLVEEAIEQDKVVALVNSAGTS
                     QTIGVMQSRVLNRHKVPLIGVFSGSGAIRGPGSEEIFHTRLTYHDEVLKIARLASTLG
                     LKKVAVLHQDDAFGEAILKSVAMAEKEYGLEVIAKAAYQPGTKDFAAQARQIVKARPQ
                     AIFLMSVPDATYQFMKAYDAPHGASQIYALSFVTPKMLAETAGEAKARGVGISQVVPN
                     PNATSMPLVKDFNAFLSSPWGKGIDASPVTLEGYLNVRLAVEAIRAAGPRPTGEKVMQ
                     SLAAMRNHQIGGFPIDFSAGRRSGSSYLDIAVVGRNAKLMY"
     gene            6607..7284
                     /locus_tag="VM406_15840"
     CDS             6607..7284
                     /locus_tag="VM406_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77484.1"
                     /translation="MRKTKLLLGALTAAFALNADAVVSVPMAQLPYTNPQQVRSMLQD
                     LDRKFSWDKTRRLIEKYVPPEIDVIGHPRGWLSNRTVCYENRTAAHCRAALSQILYTM
                     ELKEELGWTRMRIITFEQIPYSDPAPLDAIIATLDYGNGPPAMTHEQATAALEPYLAP
                     TLIVDAPAESSFNFNRAQCLYGQYANLPNPAPYFVYCHAYAVELSAILRENMSRQRAV
                     SANPFSR"
     gene            7511..8716
                     /locus_tag="VM406_15845"
     CDS             7511..8716
                     /locus_tag="VM406_15845"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL77485.1"
                     /translation="MIAKTEKTTVLLLASAQALFQTAAVMVMTISALVGFALAPNKAL
                     ATLPIALMTLSTAAMMIPAALLMRRYGRKAGFITGAVFGSIAGLLAASAVVAGSFWVF
                     VAANMLVGAYQAFAQYYRFAAADAASADFKSRAVSWVIAGGVVAAVAGPNIARFTEGI
                     GPAPFAASFLAMTALGLAAIAIISRLSLAREAAADSAGVAERPLREIARQPVFLTAVA
                     ISAVGFAVMIMVMTATPLAMRHHGHDTGSAATVIQWHVLGMFVPSFFTGNLIRRFGVL
                     RIASAGALIIIAHIGIALSGAAFLHFLSGLILLGIGWNFLFIAGTTLLTDAYRPSERF
                     KAQATHDFVMFGAVATASFSSGALLDAFGWTAVNLAALPFLVLILVAIAGYGIASRRA
                     PLAHRSNAR"
     gene            8798..>9289
                     /locus_tag="VM406_15850"
     CDS             8798..>9289
                     /locus_tag="VM406_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77486.1"
                     /translation="MELHAHQIALFETSPPFNRRMRPLVAVKNLGTTIGARGWFAILL
                     WPLLAALIAAVGWLATLRQIEAIEGEGRQAIEARARADADSYAMQLKHTVEQIDQVIL
                     RLKYEREVPHVPVDLKREREYGIFPENQHVYANIYDAGGTLVTSSTQRSAFDVSRLPY
                     FVLH"
BASE COUNT         1674 a         2986 c         3035 g         1594 t
ORIGIN      
        1 ggcgcggacg catccagcgc cgcaggttgc gcttgatgtg ccggatttcg tcgaccagcg
       61 ggaacagttc gagcagctgc gtctcgtgcg acgaacgatt gccgaagtcc gcatgcagcg
      121 cgtcgatcag gcgctgctcc cattgcagga ctccgttcag cagcgtgtcg agcgcctcga
      181 gccgccgggc gaggggcagc ggcgcgtgcg cgcgcgatgc ggcgtgctgg cgttcgaaga
      241 catcctgcag ggcgcttttc acaagtgccg gcgtgtcatt cttcatgcag gtacctccgt
      301 gccggttgca tcccaacgct gtgcgctctt cggggggaca tggctcatcg cgtgttcggt
      361 aagggcggtg atggtcaggc tgggattgac gccgaggttc gcgccgagca tcgatccgtc
      421 gcagatgtac atgttgtgat agccgaacac gcggttcttc ccgtcgcaca ccccatcggc
      481 cggcgatgcc gccatcgccg cgccgcccat gcaatgcgcc gtcatcggga tgttcagcag
      541 gatttcagtg atcgaggtct gcgcgactcc gccggttgcc tgcgccgcct tcattgcaaa
      601 atcgttcgcc gcaggaatga aggtcggaat gcgttcgccg tgcgtggcca gctgcttcga
      661 aaacggccag aaccagcgcc gtttcaggcg catggtcagg tgtccgtcga gcgtctgcat
      721 gcacaggaag atgatggatt cgcgcgccga accgaacggt gagagcacac gcagcgtcga
      781 aatcggcctg gtcacgagca gccgggccag ggtcgcaagc caggtgagaa tgcgtgtcgg
      841 ccccggccgt ccgagcgtca tcaccgtgga gagcattccc atgctgtcgg agcctttcgg
      901 atagcggacc gcttcgatat gcgtatgctc gtcgatgtag acgcccgagc cgatcgccac
      961 gccctcggac agatctgtcc tggttccggg gaaccggatg ccgatcaggg actcggcatt
     1021 ggtgcgcaca tgccttccga gagcgtccga gacgcgcggc agcgaacgct tttccttcag
     1081 ccggaacagc agttcctgcg tgccgagcga ggaggcggcg aataccacgc cgcgacaggt
     1141 gaggcgccgt ttcgcgccgc gtgtgtcgcc agcgagcgag acggtctgca cttcatagcc
     1201 ggccgatccg tcttcccggg ctgccagcgg ccgcacatcc acgacccggg tttcagccag
     1261 taacgctgcg cccttctttt cggcgaggta gaggtagttc ttgtccagcg agttcttcgc
     1321 gttgtggcgg cagccgacca tgcagccgcc gcaggcggta catgaattac gcgcagggcc
     1381 ctcgccgccg aagtaggggt cggcgtaggc gcttcccggc gggtcttcgt ccttgccgaa
     1441 gaagatgccg acttcggtgg gatagaagct gtcttcggcg ccgacgagcg ccgccatctc
     1501 ccgcaacttc aggtcggccg gcccgagccg gcgattggtg gtcacaccca gcatgcggcg
     1561 cgcggtcgcg taatgcgcgg gcatgacccg ttcccaatcg ttgagtccgg cccagctgcc
     1621 ctggcgccac accgtgtcgg gagggacgag cagggtgttg gcataaacaa tcgagccgcc
     1681 gcccaccgca ttgccatgaa ggaccacgac atgacggaag aactgcatgc tgaaaaagcc
     1741 gcgcatgccc agtgcggggc gccagaccca gcgcgcgaca tcccaattgt tctttggcat
     1801 gttgtccgcg gtccagcgct ttccctgttc gaccaccgcg acacggtagt ccttttcgac
     1861 cagacgcagc gccgaaacgc tgccgccgaa gccggagccg attacaacga agtcgtagtc
     1921 gtagtcgtag tcgtagtgca tctgaaatac cgtcgtgggt gaagatgcct ggagggctgc
     1981 agacgtcctg gaacgctgcg tcgaaaatag taacaggtgc aggtgctttg ccgcgtgttc
     2041 aggcctgcgc cgggcgagtg ctcgcgcctc aggggggcgt gttgagcacc ggctcgggaa
     2101 acgcttcttg gccgcacccg ggcgcgcatc agcagcccgt tgcggggccg tccacctctc
     2161 gcaactgcag cacgtaagag accgggcgcg gcgcactgcc atattgttcg aagcgcagca
     2221 tggccggaag gcgccggctc accaggaccg gcgcgtgatg gtggacgaag ccggcgccgt
     2281 gcacactcag ccgtacccgc ggcaggccgt cgctgccgta gcccagcacc acgtcccggt
     2341 actggaagcg cgcgtccggg cactcgaagg cgcggggcaa atccggttcg agcagcatcg
     2401 acatccggtc gaccgggttc cctcgctcgt cgactgtgcg cagttcaatg tggcgcgcag
     2461 ccgcagacgg cttaccgtcg accggaagct cggccgccgc ggcggccagc agcggatcga
     2521 ccgaacggac cgccgagacc ggatcgaacg gcagatcccg cccgggccag ggattgtagg
     2581 cgaatggcag ttgcacggcg taggcaaccg gctgatccag gccgtccacc ttcccttgga
     2641 tcgacgacag acgctgggga tcgaacgcga gctttgcgta ccgatcgctg cgggtgcgca
     2701 caaacagggt ggcggtcgtg ttcccgtcca gctccacgtg acgacggtag ccgctgtccg
     2761 gcgcgcgcgc cgcatagcgc agaagatgcc cgtaggaggc ggtggcgatg ccctgagcct
     2821 gctgcgtgag cagctgcacg cctcctcctg gcgccgcgag cgccaacccg aggggcgcgc
     2881 gcctgacatg ctcggggaag agatcagcga gggcggggtc ggcagtcgga cggttttcga
     2941 cgaagctcca cccgaaaggc tgcccgtcgg ccagcaggcc gaaagtcgcc aggccgccgg
     3001 gcacgctttg cggcggcacg ggcatggctt gcaggcgcag tgcggcgccc gcatgggctg
     3061 ccacttcggt cagacgctga tagccgttgt gcgcgacctc cagacgtgca cggcgcccgg
     3121 caagcaaggg tgcgggcagc gagagcgtga agcggccgtc ggcctgcgta cgcgcgctgg
     3181 cggcgtagct gcggtcccat accagctgag cgccggagac gccccagccg cgctgcgcga
     3241 gcgtgacatg cgcgccctcg atcggctcac cgctgacaaa gtcgaccagc tggccgctta
     3301 ccggcgcgcg gctgcagccg gacaggagca acgcaagcag aaggattgcg gctaacgggt
     3361 aacggcgggt ctgcatgtgc acagggcggc gatggaagat ctgcgcggac ttgatgatcg
     3421 cgaagcatac caagagtcga tgtggccctg aacctgtcgg tccgcatctc ttcccggcgc
     3481 gcacgcacac cgcgcatgcg cgcggtacgc cgcaaaccgc atttgacggg ccacaaaccg
     3541 accgcgcaat ccgccttgcg ttgactgcac ctcggccgat gctgagacga ccgatgtcga
     3601 tttttccagg ccctcctttc cgccccacag cctgcaagcc ctgaatgcgc atcgcacctt
     3661 cggcgcagat gtccggcccg cattcattcc gcagcaagga ggcaatcatg gctcgtcgca
     3721 aatccctgtc ccaggcgccg ttgcgcgctg atcaaccgtc cgctgaaacc gcggcgtcca
     3781 cgggttcaca agaaggcgcg gcatcgcggg catcgtcgat ccgcgttgcg atcgtgctgt
     3841 ccgcgctcgt cggcggcgtc ttctgcgtgc tcgccgacct gatgcagaag tccgatgcgt
     3901 cggcggtcga acacctcggc cgcaaactcg gcgagacgtt ctccctggtc cagggcgcca
     3961 cactgctggc gacgctgacg gtgctcgtgc tggcagtggc tgtgcccctg atcttcgagt
     4021 ccagcaccaa gaccagtgcg ttttatcttg gcgcgagcgt cctcgcgatc ctgatgacga
     4081 tgacgcccta cgagccgcca tccggcttcc agaccttgcc caactcggtc gaagtcctgc
     4141 tctcgatcga gacggcggac ggaaagccgg cgaacggtgc gcgggtaacg ctgcgcgacg
     4201 ccggcggcac gcagatcatc tcgcgcagca ggctgccgaa atcggacctg cgcttcttcc
     4261 aggacgaagg cgtctatcgg ctgactgtcg aactggccgg ctacgaggtc gcgacagagc
     4321 gcctggtgct gaaggaaggt cagccgccgc agacgatgaa ggtcaagttg cagccatcgg
     4381 acaagccgcg gatcctgcag cgcattcttc aatagcgcgt ggccgtgcgc aatttgttca
     4441 gtacacccag ggaatcaagc gcttcacccg gcttcggtaa gccggatact gcggaaattt
     4501 ctcttcgagc cagcgctcct cgcgcgcggc cttgcggtcg aagaagacga acaggatgag
     4561 tgtgagcccg aggcgcagcg ggctcgccgt caccagcgag ataccgagcg caaccagcag
     4621 gacgccaagg tagatcggat ggcgcacgag gcggtaaggg ccggtggtga cgagttccgc
     4681 atgttcgaga ggacggggaa acggcgtcag cgagcggccc agtgcgcggg cgctccacag
     4741 ggtgagcacc aggcccagca cgaacagaac ccatcccgtg aacaggaata ccgcgtcata
     4801 cggccagggg ccggcgacct gaggcaccgt gatgaaggca atgaacagga tggcctgcgc
     4861 ggcgacccag gattctcctc gctttcccat ctcatacccc cgtttcatgc atctgtccgt
     4921 gaatgaccgg caaatggatg ccgaagtgcc ggcatcgcgc atgcgcggcg caggttgcgc
     4981 cgagctgttt caaattcgcc acactttccg gcatttcgag gcagccctct tggctttttc
     5041 tcaacgggaa attgaatgag agcatgccgg acggtagatt gcacgtgctg accgttgcag
     5101 gaaaagcaat ggaagcggtc catcacaacg ataaaacggg cctgaacggc gggctcgcaa
     5161 ctggaggaga aggcatgaac atcaagcacg ttttcgtggc atgcatcgcg gccggcatgg
     5221 cggccggcgc ttgcgcggac gagatccgca tcggcatcgt ctccggcatg accggcccga
     5281 tcgccgaaac cgcggaacaa attgccgccg tctcgcgcgg ctacgtggac atgatcaacg
     5341 cccggggcgg catcaacggc agccgtcttg cgctcgtcat caaggacgac cagtacgatc
     5401 cgaggaagac ggcgccgctg gtcgaggagg cgatcgagca ggacaaggtg gtcgcgctgg
     5461 tcaacagcgc cggcaccagc cagaccatcg gcgtgatgca gagccgcgtg ctgaaccggc
     5521 acaaggtgcc gctgatcggc gtgttcagcg gctcgggcgc gatacgcgga cccggctcgg
     5581 aggaaatctt ccacacccgg ctgacctacc acgatgaagt gctgaagatc gcgcgcctgg
     5641 cgtccacgct cggactgaaa aaggtcgcgg tcctgcacca ggacgacgcg ttcggagagg
     5701 ccatcctcaa gagcgtggcg atggcggaga aggagtacgg tctggaggtc atcgccaagg
     5761 cggcctacca gcccggaacg aaggacttcg ccgcgcaggc gcgccagatc gtcaaggccc
     5821 gtccgcaggc catcttcctg atgagcgtgc ccgacgcgac ctaccagttc atgaaagcct
     5881 atgacgcgcc gcacggcgcg tcgcagatct acgcgctgtc cttcgtgacg ccgaagatgc
     5941 tggccgagac cgctggcgag gccaaggcgc gcggcgtcgg catctcgcaa gtggtgccca
     6001 acccgaacgc cacctcgatg ccgctggtca aggacttcaa cgccttcctc agcagcccgt
     6061 ggggcaaggg catcgacgca agtccggtca cgctcgaagg ctatttgaat gtccggctgg
     6121 cagttgaggc gatcagggcg gcagggccgc gtcccaccgg cgagaaggtc atgcagagcc
     6181 tggcggccat gcggaaccat cagatcggag gctttccgat tgatttctcg gccgggcggc
     6241 gcagcggctc gagctatctc gacatcgccg tagtcgggcg caacgcgaaa ctgatgtact
     6301 agtgacccga gtcattaatt cgttgatgaa aaccgatgaa atcgacccca tgcggcgttg
     6361 ccaatcctcg cgatagtccc actatcgctg cggttggcgc ctggcctggc gccgattgca
     6421 tcaattttct tattcaacaa actcgcgact caggtcacta aagctgcggg gccgttcacg
     6481 gcccctgcgc ttgccaagcg gcgacacctg gacgatgcgc tgcgccgctt gcgtcagcgc
     6541 aaagggcagc gatggcgttt tcctacagtt gcggaacatg ttgtcgcaac ccgccgggaa
     6601 aacgccatgc gcaagacgaa gctgttgctg ggtgccctga ctgccgcatt tgccctcaac
     6661 gccgatgccg tcgtctcggt gccgatggcc cagctgcctt acacgaatcc gcagcaggtt
     6721 cgcagcatgc tgcaggatct cgaccgcaaa ttttcctggg acaagacccg gcgtctgatc
     6781 gagaagtacg tgccgccgga gatcgacgtc atcggccatc cgcgcggctg gctgtccaac
     6841 cgtaccgtct gctacgagaa ccggacagcc gcgcattgcc gcgcggcgct aagccagatc
     6901 ctgtacacca tggagctcaa ggaagagctc ggctggacgc gcatgcgcat catcacgttc
     6961 gagcagatcc cgtatagcga tcccgccccg ctcgacgcga tcatcgccac cctcgattac
     7021 ggcaacggcc caccggcgat gacgcacgag caggcgacag cggctctcga accgtacctg
     7081 gccccgaccc tgatcgtcga cgccccggcg gaaagcagct tcaacttcaa ccgcgcgcaa
     7141 tgcctctacg gccaatacgc gaacctgccc aatccggcgc cgtatttcgt ctactgccat
     7201 gcgtacgcgg tggaactgtc cgccatcctg cgcgagaaca tgtcgaggca acgggcagtg
     7261 tcggccaatc cgttttcacg ttaggcgggc cgcctgaccg caggcgcggc ccgccggcat
     7321 cattgcgcca cgacactggc cttcaattgt ctccggccga ttggtgccgc caccgggcgg
     7381 ctcggcagca ccactctagg aatcgttaga atgatgttac gttcggcgca ttggaggagc
     7441 gatgcgtccg gacaagcgca ttgatcggac gaactggtcc gacaaggtca tcgttatcca
     7501 ggagacgcct gtgattgcca agacggaaaa gacaacggtg ctgctgctgg cgagcgcgca
     7561 ggcgctcttt cagacggccg cggtgatggt gatgacgatc tccgcgctgg tcggcttcgc
     7621 gctggcgccg aacaaggcgc tggcgacgct gccgattgcg ctgatgacgc tctcgaccgc
     7681 cgcgatgatg attccggcgg cgctgttgat gcgccgctac ggcagaaagg ccgggttcat
     7741 caccggcgcc gtgttcggtt cgattgccgg cctgctggcc gcgtcggcgg tcgtggcagg
     7801 atcgttctgg gtgttcgtgg ccgccaacat gctggtcggg gcgtatcagg cctttgcgca
     7861 gtactaccgc ttcgctgcgg cggatgcagc cagcgccgat ttcaagagcc gtgcggtatc
     7921 gtgggtgatc gccggcggcg tggtggccgc ggtcgcgggg ccgaacatcg cgcggttcac
     7981 cgagggaatc ggcccggcgc cgtttgcggc gtcgttcctg gcgatgacgg cgctcggact
     8041 ggcggcgata gccatcatct cgcgcctgtc gctggccagg gaagcggcgg cagacagtgc
     8101 cggcgtggct gaacgtccgc tgcgcgagat cgcccgacaa cctgtcttcc tgacggcagt
     8161 tgcgatatcg gcggtcggat tcgcggtcat gatcatggtg atgaccgcca cgccgctggc
     8221 gatgcggcac cacggtcacg acacgggttc agccgccacc gtcatccagt ggcatgtgct
     8281 cggcatgttc gtgccgtcct tcttcactgg caacctgatc cgccgcttcg gcgtgctgcg
     8341 catcgcatcg gccggcgcgc tgatcatcat cgcgcacatc ggcatcgcgc tgtccggcgc
     8401 cgccttcctg catttcctgt ccgggctcat cctgctcgga atcggctgga acttcctgtt
     8461 catcgccggg accacgctgc tgaccgacgc ataccggccc agcgagcgtt tcaaggcgca
     8521 ggcaacccat gatttcgtga tgttcggtgc ggtcgcaacc gcctcgttct cgtccggagc
     8581 cttgctcgat gccttcggct ggacggcggt taacctggcg gcgctgccgt tcctggttct
     8641 gatcctggtt gcgatagcag gctatggaat cgcaagccgg cgcgcgccgc ttgcgcaccg
     8701 gtcgaacgcg cggtgacccg ccgaaacgac gccaccggtt ccggggtggc agtcggagct
     8761 gtggccgcag tgggggacgg aggccgaatc gggccgcatg gaacttcatg cgcatcaaat
     8821 tgctctgttc gaaaccagtc ctccgttcaa cagacgtatg cgccccttgg tcgccgtaaa
     8881 aaaccttggc acgaccatag gtgcgcgtgg ctggttcgcc attctccttt ggcccctgct
     8941 cgccgcgttg atcgcggcgg tcggctggct cgcgacgctc aggcagatcg aagcgatcga
     9001 gggcgagggg aggcaggcga tagaggcgcg cgcgagagcc gatgccgact cttacgcgat
     9061 gcagctcaag cacacggtcg agcaaatcga ccaggtcatt ctgcgcctca agtacgaacg
     9121 ggaagtgccg cacgttcctg tcgatcttaa acgtgaacga gaatacggaa tctttccgga
     9181 gaaccagcac gtatacgcga atatctatga cgccggcggg accctggtaa ccagcagcac
     9241 gcaacggagc gccttcgacg tatcgcgcct gccgtatttc gtgctgcac
//
LOCUS       DATLGF010000509         6547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_219557, whole genome shotgun sequence.
ACCESSION   DATLGF010000509 DATLGF010000000
VERSION     DATLGF010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219557"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1869
                     /locus_tag="VM406_15855"
     CDS             <1..1869
                     /locus_tag="VM406_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL77487.1"
                     /translation="ELDLPMNPRTRARRGAARIAALMVAVAIVAGVYATAEHRMRQDI
                     HAAGARQLQIIALDLESLLERFESLPAAIAFHPNLARLLADPRNAQLAGQLNQTLQSI
                     QQLSKVSAIYLMDAEGNTLVSSNWDTAQTFVGRNFAFRPYFRDAIEGRAGHFYGIGSV
                     TSQPAYFIAQPVYPDGARAPLAKPAGVIAVRISLTEFEQSWRSSEDPIALADRIGVVF
                     LSNRPEWRYHSMRQIDEGDRRYLAGTLQYVGKSILPISALPKEQQAGFGGHVSQQVGR
                     LGWQLMLFPSQQRAARGAGYAALAAALLLSLVALGWWANHQRARRLEEGRASRRALQQ
                     AAEELDRKIAQRTEELLQANRHLEARYATLQQTEQMLRTAQNELVQAGKLAMLGQMAA
                     GVTHELNQPLAAIRAFADNAVTFLQRGRTDAASDNLSQISAAAARMGAIIAQLKGFAR
                     KSRDTVATVDLASIIHASAALLDSEFRRHQAALAIEIDEALPVTGDAVRMEQVLINLL
                     RNALDAVENGAERNVALHARRDEAYAVVTIRDSGPGITEEVRQHLFEPFFTTKPSGKG
                     LGLGLAISSSIVQAMNGKLSARNHPQGGAEFELDLPLAPGDPCTAGAEEGKRVANI"
     gene            1853..3184
                     /locus_tag="VM406_15860"
     CDS             1853..3184
                     /locus_tag="VM406_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HVL77488.1"
                     /translation="MLQTFEAVLVEDEQTLRHATAQTLELGGFTVHACSSAEEARGWL
                     HEDFAGVLVTDVRLPGRSGLELLADVVALDSELPVILVTGHGDVSMAVEAMRAGAYDF
                     VEKPFAAEGLLETVRRAQEKRRLVLDNRRLKAAWSAHPDMPRLIGESAPIERVRKLIR
                     AIGPTSADVLINGQTGTGKEVVARHLHAASGRKGPFVAINCGALPESVFESEIFGHEA
                     GAFTSAQKRRIGKLEYANGGTVFLDEIETMPLVLQIKLLRVLQERRLERLGGNDPIDV
                     DCRIIAASKADLLQLAAEGRFREDLYYRIGVVSIDLPRLAERREDIPLLLAHFAQGAA
                     LRHHRPVPTWSAAQMAQWQARDWPGNVRELRNFADRLVLGVIGSVAAEPRDEGSSLPQ
                     RVDAYERMLVAEALGDHAGNVAAAAEALGVPKKTLYDKLKKYQLGSSRAQE"
     gene            3256..4065
                     /gene="ccsA"
                     /locus_tag="VM406_15865"
     CDS             3256..4065
                     /gene="ccsA"
                     /locus_tag="VM406_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HVL77489.1"
                     /translation="MHTTFFVLAALLYGVGALLPSRQRTAISIVTAAGWLLHGAALGL
                     DFIGPAALRVGFAAVLSATLWISVAAYWLENRNMALDGMRVLVLPSAALTVLLPLAFP
                     GTVVPLEGRSILFPWHVAIAILAYSTLTIAAFHAVLMALQESRLHGHSARASRNWLGL
                     AIDRLPALLTMERLLFRLIALGFVLLTLTVFSGVLFSEYLFGTAFKWDHKNVFTLLSW
                     LLFGILLAGRVWRGWRGKTALRFTLTGFSTLLLAYVGTRFVLEVVLMRGSA"
     gene            4062..4301
                     /locus_tag="VM406_15870"
     CDS             4062..4301
                     /locus_tag="VM406_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HVL77490.1"
                     /translation="MKFLLWALLIAAVVLWVLHVRRSATPGEGRDGRRAGDGKQAEPM
                     VQCARCGVHVPVSESVGDQSGRVFCSSEHRRLGCD"
     gene            4371..6038
                     /locus_tag="VM406_15875"
     CDS             4371..6038
                     /locus_tag="VM406_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVL77491.1"
                     /translation="MLNAAVPLPRGTRDTFWRSLQTFGITRIVITVLLLLYLTYNPGK
                     EARTLAVALGWELGAIYLAAALGFALVSLYVRQHFMAQLLAQLTVDIAMITKLYVTAG
                     GASSGMAILYLFPLAGCAILAPLVLALFTVSVVTLILLGESGYQMLRSAGAASTSQAG
                     LYGAAFFAAAYMVNRLAAHLIRQEDLAAKRGRELHLQEAINRLVVADMNEGVLVVGPK
                     TEVMTGNPAAARMLGQPTLHTGFRLREHAGLAPIVQALKSWKSGFPDSGQVDGSCFVM
                     VKSEPASGSSDEAHGPQYPLAAHLKLRFMKVETGEPNEERSVIFMRDVSEIENQAQQL
                     KLASMGRLTASIAHEVRNPLSAISHAASLLREDVVDPTHARLLAIVTENALRLDRMIE
                     DILKLSRKAQVHDKPLQLGDVLDGIVSEFCAAQSLDPSVVSIEDMRGYRVHFDPLHLR
                     EIVVNLLSNAMRYASGGPGSIRVRARRPRKGRFEIHVQDDGTPITPQVRAHLFEPFYT
                     TSSKGTGLGLYVARELCLNNGAMLDYEYRSDPGSAEPTGRFVITFAN"
     gene            6048..>6547
                     /locus_tag="VM406_15880"
     CDS             6048..>6547
                     /locus_tag="VM406_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL77492.1"
                     /translation="MTSPRILVIDDEADLRELLELTLLKMGLEVSCAATLAEAYAALD
                     HEEFSLVLTDMRLPDGLGIDLVRELSSRPRHPPVAVITAYGSADNAVAALKAGAFDYL
                     SKPLRLEELRLMVQSALRLGKDAGAPGTRAKSGGESARLIGESDVMRDLRTQIQRLAR
                     SMAPVA"
BASE COUNT         1096 a         2110 c         2169 g         1172 t
ORIGIN      
        1 gaactggatc tgccgatgaa cccccgcacg cgcgccaggc ggggcgctgc ccgcattgcg
       61 gcgctcatgg tcgctgtcgc catcgtggcc ggcgtctatg cgacagccga acacaggatg
      121 cgccaggaca ttcatgccgc cggcgctcgg caactccaga tcattgcgct ggacctggag
      181 tccttgctgg aacgcttcga gagcctgccg gcagcgattg cctttcaccc gaacctggcg
      241 cgcctgctcg ccgatccgcg caatgcgcag ctggccggcc agctgaacca gacgctgcag
      301 tcgatccagc agctgtccaa ggtcagcgcc atctatctga tggatgccga gggcaacacc
      361 ctggtgtcga gcaactggga caccgcgcaa accttcgtcg gccgcaattt tgcatttcgc
      421 ccctactttc gtgatgcgat cgagggacgc gccggtcact tctacggtat cggcagcgtc
      481 accagccagc ctgcctattt cattgcccag cccgtctacc cggacggcgc gcgcgcgccc
      541 cttgcgaagc cggccggcgt gattgcggtg cgtatcagcc tgacggaatt cgagcagagc
      601 tggcgcagca gcgaggatcc gatcgcgctg gccgaccgga tcggagtggt cttcctcagc
      661 aaccgtcccg agtggcggta tcacagcatg cgtcagatcg acgaaggcga ccgccgatac
      721 ctggcaggca cgctccaata tgtgggcaag agcatacttc cgatttccgc gctgccgaag
      781 gaacagcaag ccggtttcgg cgggcacgtg tcgcagcagg tggggcggct cggatggcag
      841 ctgatgctgt ttccatcgca gcagcgcgct gcgcgcggag ccgggtatgc ggcgctcgcg
      901 gcggcgctgc tgttgtcgct ggtcgccttg ggatggtggg ccaatcatca gcgcgcgcgc
      961 cggctcgaag aaggccgggc ctcgcggcgt gcattgcaac aggcggcgga agagctggac
     1021 cgcaagatcg cccagcgtac cgaggaactg ctgcaggcaa accgccacct cgaagcgcgt
     1081 tacgcgacgc tgcagcagac cgagcagatg ctgcgcaccg cacagaatga actggtgcag
     1141 gccgggaagc tggcgatgct gggccagatg gccgccggcg tcacgcatga actgaaccag
     1201 cctcttgcgg caatccgcgc gtttgccgat aacgcggtca ccttcctgca gcgcgggcgc
     1261 accgacgccg ccagcgacaa cctgagccag atcagcgcgg cggcggcgcg catgggagcg
     1321 atcatcgccc agctgaaggg atttgcgcgc aagagccgcg acaccgtggc gacagtcgat
     1381 ctcgccagca tcatccatgc ttcggcggcg ctcctcgata gcgagttccg ccgccaccaa
     1441 gcggcgctcg caatcgagat cgacgaagcc ctgccggtga cgggcgatgc cgtgcggatg
     1501 gagcaggtgc tcatcaacct tttgcgaaac gctcttgatg cagtggaaaa tggcgccgag
     1561 cgcaacgtgg ccttgcacgc gcgccgcgac gaagcgtacg cggtggtgac gattcgcgac
     1621 agcggacccg gcattaccga ggaggtgcgc cagcatctgt tcgaaccgtt cttcacgacc
     1681 aagccgtccg gcaagggcct gggcctcgga ctggcaatct cgtcgtcgat cgtgcaggca
     1741 atgaatggaa aactgagcgc gcgcaatcac ccgcaaggcg gggcggaatt cgaattggac
     1801 ctgccgttgg cgccgggcga tccgtgcacg gcgggagcgg aggaaggaaa acgtgttgca
     1861 aacatttgag gcggtgctgg tcgaagacga gcagaccttg cgccatgcga ctgcgcagac
     1921 gctggaattg ggcggcttca ccgtccatgc ctgttccagc gccgaagagg cccgcggctg
     1981 gctgcacgag gatttcgctg gcgtgctggt cactgacgtg cgcctgcccg gacgctccgg
     2041 cctcgagttg ctcgccgacg tggtggcgct cgattccgag ctgcccgtca tcctggtaac
     2101 cgggcatggc gacgtcagca tggcggtgga ggcgatgcgc gccggtgcct acgacttcgt
     2161 ggagaaaccg ttcgccgcgg aaggcttgct cgaaaccgtg cgccgcgccc aggaaaagcg
     2221 ccgcctggta ctggacaacc ggcgcctgaa ggcggcctgg agcgcccatc cggacatgcc
     2281 gaggctgatc ggtgaatccg ctccgatcga gcgggtgcgc aagctgatcc gtgccatcgg
     2341 acccaccagc gccgacgtgc tgatcaatgg acagaccggc accggcaagg aagtggtcgc
     2401 gcgccacctg catgcggcga gcgggcgcaa gggcccgttc gtggcgatca actgcggcgc
     2461 gctgccggaa tcggtgttcg agagcgagat tttcggccac gaggccggcg cgtttaccag
     2521 tgcgcagaag cggcgtatcg gcaaactcga gtacgccaat ggcggcacgg tgttcctcga
     2581 cgagatcgag accatgccgc tggtcctgca aatcaagctg ttgcgcgtgc tgcaggagcg
     2641 gcgcctggag cgcctcggtg gaaacgatcc gatcgacgtc gactgccgca tcattgcagc
     2701 gagcaaggca gacctcctgc aactggccgc cgaagggcga ttccgcgagg acctgtacta
     2761 ccggatcggc gtggtgagca tagacctgcc gcgcctggct gaacggcgcg aggacatacc
     2821 gctattgctg gctcacttcg cacaaggcgc ggcgctgcgt caccaccggc cggttccgac
     2881 gtggagcgcc gcccagatgg cgcaatggca ggcgcgcgac tggcccggaa atgtgcgcga
     2941 gttgcgcaat ttcgccgacc gtctggttct cggcgtgatc ggatcggtgg cagccgagcc
     3001 cagggacgag ggaagcagtc ttcctcagcg cgtcgatgcg tacgagcgca tgctggttgc
     3061 cgaagcgttg ggggatcatg ccggcaacgt cgcggcagcg gcggaagcgc tcggcgttcc
     3121 aaagaagacg ctgtatgaca agctgaaaaa atatcagctc ggcagcagtc gcgcgcagga
     3181 atagggcgca acgagggtct cgggaaccgt gcaccggtgg tgtacactcg tggcctgctt
     3241 tcccacttcg tgacgatgca caccacgttt ttcgtccttg ccgctcttct ttatggcgtc
     3301 ggcgcgctct tgccgtcaag gcaacgcacc gccatttcca tcgtcacagc tgcagggtgg
     3361 ctgttgcatg gcgccgcgct cggcctggat ttcatcgggc cggccgcgct gcgcgtcggc
     3421 ttcgctgcgg tcctgtcggc cacgctctgg atctcggtgg ccgcctactg gctggaaaac
     3481 cgcaacatgg cgctggacgg gatgcgcgtg ctcgtgctgc cttccgcggc gctgaccgtc
     3541 ctgctgccgc ttgcatttcc cggcaccgta gttcccctgg aggggcgctc gatcttgttt
     3601 ccctggcacg tggcgattgc catccttgca tacagcaccc tcaccatcgc ggctttccac
     3661 gcggtgctga tggcgctcca ggagtcgcgc ttgcatgggc attccgcccg cgccagtcgc
     3721 aactggctcg ggcttgcgat cgatcgcctt cccgcgctgc tgacgatgga acgcttgctg
     3781 ttccgcttga tcgcactggg tttcgttctg ctgacgctca ccgtgttttc cggggtgctg
     3841 ttttcggaat atctgttcgg cacggcgttc aagtgggatc acaagaatgt cttcacgctc
     3901 ctgtcatggc tcctgttcgg catcctcctc gccggtcgcg tgtggcgggg ctggcgcggc
     3961 aagacggcat tgcgcttcac gctgaccggc ttttcgaccc tgctgctggc ctacgtcgga
     4021 acgcgcttcg tgctggaggt ggtgctgatg agaggcagcg catgaagttt cttttgtggg
     4081 cgctgctcat cgccgctgtc gtgctgtggg tcttgcatgt gcgcaggtcg gcgaccccgg
     4141 gcgaggggcg agacggtcgt cgggcgggtg acgggaagca ggccgagccg atggtgcagt
     4201 gcgcgcgctg cggtgttcat gtacctgtct cggaatcggt cggcgaccag tctggccgcg
     4261 tgttctgctc cagcgagcac cgccgtctcg gctgcgactg agatgcgcac cgtagtgacc
     4321 ttctccacga tgcttgcccc tggccgtgcc tcttggcgca ggacatgcac atgttgaacg
     4381 cggccgttcc cttgccgcgg ggcacgcgtg acacgttctg gcgttcgctg cagaccttcg
     4441 gcatcacgcg catcgtcatc acggtgctgc ttctgctcta cctcacctac aatccgggca
     4501 aggaggcgcg caccctcgcc gtcgcactcg gatgggagct cggcgccatc tacctggccg
     4561 cggcgctcgg cttcgcactg gtttccctgt atgtgcgcca gcacttcatg gcccagctgc
     4621 tcgcccagct caccgtcgat atcgcgatga tcaccaagct gtacgtcacg gcgggcggcg
     4681 cgagcagcgg catggcgatc ctctatctgt ttccgctggc cggctgcgcg attctcgccc
     4741 cgcttgtgct ggcattgttc accgtgtccg ttgtcacact gatcctgctc ggcgagagcg
     4801 gctatcagat gctgcgctcg gccggtgcgg caagtacctc ccaggcaggg ctgtatggcg
     4861 cggcattctt cgccgcggcc tacatggtga accgcctggc cgcgcacctg atccggcagg
     4921 aggatctggc ggcgaagcgc gggcgcgaac tgcatttgca ggaagcgatc aaccgcctcg
     4981 tggtcgccga catgaacgag ggcgtactcg tcgtcgggcc gaagaccgag gtgatgaccg
     5041 gcaatcccgc cgccgcgcgc atgctcggcc agcctacgct gcacacagga ttcaggttgc
     5101 gcgagcacgc cggacttgcg ccgatcgtgc aagccttgaa atcctggaaa tcgggatttc
     5161 ccgattcggg gcaggtcgac ggaagctgtt tcgtgatggt caagtccgag ccggcgtcgg
     5221 gctcctcaga cgaggcgcat ggtccgcaat acccgcttgc ggcccacctg aaacttcgct
     5281 tcatgaaagt ggagaccggc gaaccgaacg aagagcgcag cgtgattttc atgcgcgacg
     5341 tgagcgagat cgagaatcag gcgcagcagc tcaaacttgc gtcgatgggc cgcctgacgg
     5401 ccagcatcgc gcacgaagtg cgcaatccgc tctcggcgat atcgcatgcg gcgtcgttgc
     5461 tgcgtgaaga cgtcgtcgat cccacccacg cacgcctgct ggcaatcgtg accgagaacg
     5521 cgttgcgcct ggacaggatg atcgaggaca tcctgaagct gtcgcgcaag gcgcaggtgc
     5581 atgacaagcc gttgcagctg ggagacgtgc tggacgggat cgtgtccgag ttctgcgccg
     5641 cgcagtcact cgacccgtcg gtcgtttcga tcgaggacat gcgcgggtac cgggtgcact
     5701 tcgatcccct gcacctgcgc gagatcgtcg tcaatctgct atcgaatgcc atgcgttacg
     5761 cgagcggcgg tcccggatcg atccgcgttc gtgcccggcg tccccgcaag ggtcgcttcg
     5821 agattcacgt gcaggacgac ggcacgccga tcacgccgca ggtgcgcgcg catctgttcg
     5881 aaccctttta cacgacgtcg agcaagggga ccggcctggg cctctacgtg gcccgcgagc
     5941 tgtgcctgaa caacggcgcc atgctcgact acgaataccg ctccgatccc ggcagcgccg
     6001 aacccaccgg acgtttcgtc atcacgtttg ccaactagcc tgccatcatg acttcgcccc
     6061 gcatccttgt catcgacgac gaagccgact tgcgcgaact gcttgaactg acactgctca
     6121 agatgggcct ggaggtgagc tgcgcggcga cactggccga ggcatatgcg gcgctcgacc
     6181 acgaggagtt ctcgctggta ctgaccgaca tgcgactgcc ggacggcctg ggcatcgacc
     6241 tggtgcgcga attgtcgtcc aggccccggc atccgccggt cgccgtcatt actgcctacg
     6301 gcagcgccga caacgccgtg gccgcgctga aggcgggagc gttcgactac ctgagcaagc
     6361 cgctgcggct ggaggagctg cggctcatgg tccagtcggc gctgcgcctg ggcaaggacg
     6421 ccggcgcgcc cggaacgcgc gccaagtccg gcggcgagag cgcccggctg atcggcgaat
     6481 ccgacgtcat gcgcgacttg cggacgcaga tccagcgcct cgcgcgcagc atggcgccgg
     6541 ttgccat
//
LOCUS       DATLGF010000510        11703 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_186142, whole genome shotgun sequence.
ACCESSION   DATLGF010000510 DATLGF010000000
VERSION     DATLGF010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 11703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11703
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186142"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VM406_15885"
     CDS             complement(<1..837)
                     /locus_tag="VM406_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HVL77493.1"
                     /translation="MLKRYLAAFAALTAAAGATRAQDLQTVDQAIEAALSPVSDFIAG
                     IIFYSVPVAGTEFPMIVGWLIIAGTIFTLYFGFHQFRGFGHAIALTRGDYSDKHKREP
                     GETSHFQALSMALSGTVGLGNIAGVAVAVSIGGAGATFWMILAGLLGMASKFTECTLG
                     LKYRNHNPDGSVSGGPMYYLSKGLAERGMAGLGKVLAVVFAICCIGGAVGGGNMFQAN
                     QAYQQVVNVTGGADSILAGRGWVFGLILASVVALVIIGGVRSIARVTSRVVPFMAILY
                     VGT"
     gene            complement(1203..1691)
                     /locus_tag="VM406_15890"
     CDS             complement(1203..1691)
                     /locus_tag="VM406_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL77494.1"
                     /translation="MEHQAAFRQARASDLPAIVAMLADDALGAARERVSDPLPESYAT
                     AFAHIDADPNNELIVAERDGIVVAMMQLTYIPSLSYQGSWRALIESVRVHGSMRGRGL
                     GHALIHHAVERARARGCGMVQLTTHKSRTDAHRFYQDLGFVASHEGMKRMLDTPARAQ
                     DV"
     gene            1882..3015
                     /locus_tag="VM406_15895"
     CDS             1882..3015
                     /locus_tag="VM406_15895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL77495.1"
                     /translation="MMGYKHGSAVVASAHVGLARAADDADVEVKVYENSIPPFAEREM
                     ERLYGSFYASMPQLEIEGSTRGVSTWVASRHGEIIELWLYRIEGREVRVLNEAITVTA
                     AAVRRFADTVFAGSAALDAICFNAIYPALDGLALPCQRVHCTDDTVIVLPRNEEAWLA
                     ALGKTTRKNMRRYMQRFTEAFPGYRYRVYERDAIPEAHVRAIIAFNRARMTAKGKVSG
                     VDAEQEARILKLVRRCGLIGVATIDDRIVGGAIVYQVGANYFSYLRAHDPAYNDYRLG
                     LIGGYELVRTCIGRGGRELHLMWGREPHKLLLGGEQRELDRVVVYRSRLRALRHLHRI
                     AHTAWASRTRAARAWLTELAARREGPIGHLLHSARGVVRRLRR"
     gene            complement(3038..5071)
                     /locus_tag="VM406_15900"
     CDS             complement(3038..5071)
                     /locus_tag="VM406_15900"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HVL77496.1"
                     /translation="MEHRILQVALDTPLDTLFDYRWCGEDGEPEPQAGQLVLVPFGRR
                     ESVGLVVRVGDASTLPVDKLKDVMAVRRQLPPVSPHWLGLCAFAADYYQRPLGEVALP
                     ALPKNLRTATTVSLDRALKKLAGSEHGDACCPSQPPVLNPEQQAAVDALAEARGFAAM
                     LLYGVTGSGKTEVYLQAAARVLAAAPPDEPTQILILVPEINLTPQLEQNIRARFPDVP
                     LATLHSGLAEGERLRHWLAAHLGHARIILGTRLAILASLPHLKLIVVDEEHDPSYKQQ
                     EGLRYSARDLAVWRARQLGIPIVLGSATPSLETWHHAGSGRYRRLELRRRASRAAVLP
                     AVRLIDTERDKAGEGLTATLAAALKLRLERGEQSLLYLNRRGYAPVLSCDACGWISNC
                     PRCTAFMVLHKPEHRLRCHHCSLQQRIPRSCPTCGNVDLQPLGRGTQRIEESLQQLFP
                     QARILRIDADSTRRKGSAQAAFDSVHRGEVDILIGTQMVAKGHDFRNLTLVGVLNPDT
                     ALFSHDYRAAERLFAQLMQVSGRAGRAGQKEGGSASEVLIQTRYPQHPLYGALIAHDY
                     DRFAQGLLEERHAAGLPPFVYQALLRAEARELQAALDFLTDAASCLEHPGIVIHEPIP
                     MTITRVANVERAQLLVESASRPALQAFLKEWMRLLRAMKARARWSLEVDPVDI"
     gene            complement(5708..6802)
                     /gene="hemE"
                     /locus_tag="VM406_15905"
     CDS             complement(5708..6802)
                     /gene="hemE"
                     /locus_tag="VM406_15905"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HVL77497.1"
                     /translation="MPNNFPPLKNDTFLRALLREPTDYTPMWLMRQAGRYLPEYCATR
                     KRAGSFLGLAKNPDFATEVTLQPLDRYALDAAILFSDILTVPDAMGLGLYFAEGEGPK
                     FERPLRDEQAVLALKTPEPGSLQYVFDAVTQIRTELGGRVPLIGFSGSPWTLACYMVE
                     GGGSDDFRRVKTMLYQRPDLMHHILKTNAEAVGDYLNAQIEAGAQAVMIFDTWGGVLA
                     DGIYQQFSLAYMRDVLARLKREHDGARIPVIVFTKGGGLWLEEIAAIGTDAVGLDWTV
                     NLGRAKARVGDKVALQGNLDPNVLFAGPEQIRAEVRKLLASYGAPGHGHVFNLGHGIS
                     QFTPPDAVSVLAEEVHTAGRALRAESAAPA"
     gene            complement(6905..7399)
                     /locus_tag="VM406_15910"
     CDS             complement(6905..7399)
                     /locus_tag="VM406_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77498.1"
                     /translation="MEFLFNPTFWETASYVVTVVGLPFAIFLFFYEQRKERDNEDEEA
                     YQLLQNAYNEFLEIVLANPDLQLRSTTMRADMTDEQRERALIIFEMLISLFERAYIVS
                     YDDNLSGVALRRWNSWDDYMREWCRRDDFYFLLPHLLRGEDPDFAAYIRRVADEEQST
                     KLLG"
     gene            complement(7572..8315)
                     /locus_tag="VM406_15915"
     CDS             complement(7572..8315)
                     /locus_tag="VM406_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020693810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HVL77499.1"
                     /translation="MRDYLPELVALPLLPLLLAQGRRTRRITPRLPEAPGATDGIAGR
                     HFNAAPLRMLAFGESPVAGVGVQSHEEAITGCLAQALAARLRRPVHWEARGQNGATVR
                     DAMGSLLPHVAAQPQDLVLVAFGVNDTTAFRSAGQWRQDVRDLLDALEARCAPRLMLV
                     SGVPPMGRFPALPQPLRAVMGLKSASLDRVLRALVRTRANTLHVPLPSGLDGPGLMAH
                     DGYHSSAAGCSAWAAVLADSCAAAFGHSD"
     gene            complement(8322..8759)
                     /locus_tag="VM406_15920"
     CDS             complement(8322..8759)
                     /locus_tag="VM406_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HVL77500.1"
                     /translation="MTVPTDISRLLKDSRTIAVVGLSAHPDRPSYEVAAYMQAQGYRI
                     IPVNPTYAGAHILGEHCYATLTLAAQVVSAEAGKIDIVDCFRRPEHIAAVVDEAIAIR
                     AGSVWMQLGIEDAVAAERARAAGLEVVMDKCIKIEHAALVAAR"
     gene            9156..11051
                     /locus_tag="VM406_15925"
     CDS             9156..11051
                     /locus_tag="VM406_15925"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HVL77501.1"
                     /translation="MSPLCLPRAAHKRLPFAFHVLLLALLQPPLSQANQLNILPSEIV
                     KAETRWRFSENATLDEEQWVQRRALSDEGAVQIGRQRFSFNTELEDFSILEAYTLKAD
                     GRKLLVHPDEVLVQRGLAVGGTAVSLPSVEMHQLTYPDVQKGDSVVWRYRKKSMRTAL
                     PGWASAARFAYPWESVLQEQVRIESPQALRLHVFAAGYDARKNQEDGLDVWIIEGKHQ
                     AFPIEQNWADSLQWLPRVYATTLPDHEAWALRFAEPIRQKSETTPAVRALAQRITKGH
                     ATSHEKARAVYDWIRSNIRYVAVHAGVGGYVPHEVEDILRKRYGDCKDQTLLMIALLK
                     AVGIEAAPALLYTAPEYALPELPVGTDHVIVYIPELDLFADATASTIPFGSLPWNAAD
                     KPAVVAMAEGSHRMRTPAFRADDNRLIVKSVWQIARDGSADAVIEVEGSGNAATLLQD
                     RLQQIPAGMGSVAVQRILETSRLSGRGFIHFPAVQRERQQQSMRLDLENVRNLIVDPA
                     AGSLAPHPALNLPLYILSNMGNYAAEARRHPMTCTPVRLREDFELNLDPGFDILRIPK
                     DAREEHADGIVFEARYTVNGNRLSGWRELILDHGRHSCNVDQYKERRAVMQRIDQHLR
                     GSVLYTQ"
     gene            complement(11166..11588)
                     /locus_tag="VM406_15930"
     CDS             complement(11166..11588)
                     /locus_tag="VM406_15930"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HVL77502.1"
                     /translation="MANTIHVDVVSAEEEIFAGEAEFVALPGEAGELGIYPKHTPLIT
                     RIRPGAVRIKVPGQEEDEFVFVAGGILEVQPNAVTVLADTAIRGHDLDEAKASEAKKL
                     AEEAMANNESSIDYARAQAELAAAVAQLAAIQKLRQKR"
BASE COUNT         2129 a         3775 c         3762 g         2037 t
ORIGIN      
        1 ggtgcccaca tacaggatcg ccatgaacgg caccacgcgc gaggtcacgc gggcgatcga
       61 gcgtacgccg ccgatgatga ccagcgccac caccgaggcg aggatcaggc cgaacaccca
      121 gccgcggccg gccaggatgc tgtcggcccc gccggtgaca ttgaccacct gctgataggc
      181 ctggttggcc tggaacatgt tgccgccgcc caccgcgccg ccgatgcagc agatcgcgaa
      241 aaccaccgcg agcaccttgc cgaggccggc catgccgcgc tcggccagcc ctttcgacag
      301 gtagtacatc ggtccgcccg acaccgagcc gtcgggattg tggttccggt atttcagacc
      361 cagcgtgcac tcggtgaact tggacgccat gccgagcagg ccggcgagga tcatccagaa
      421 ggtcgcaccg gcgccgccta tgctcacggc caccgcaacg ccggcgatgt tgcccaggcc
      481 gaccgtaccg gaaagcgcca tcgacagggc ctggaaatgc gaggtctcgc cgggttcgcg
      541 cttgtgcttg tccgagtagt cgccgcgcgt caacgcgatg gcatgaccaa agccgcggaa
      601 ctggtgaaag ccgaagtaca gcgtgaagat cgtgccggcg atgatcagcc agcctacgat
      661 catcggaaac tccgtaccgg caaccggcac ggaatagaag atgatgccgg cgatgaaatc
      721 cgataccggg gataaagccg cttcgattgc ttggtctacc gtctgcaagt cctgggcacg
      781 tgtggcgcct gcagccgccg tcaatgcggc gaatgccgca aggtatcgct tcaacatggt
      841 gtctcctcct aataggtcag cccgcacgct caagctgatt gtcgaaccgg aatgcagaaa
      901 atcgtgttga ccttccgcag cggatcgggc agttccgcga gatcgatcga gaagccgccg
      961 ttcacgtgca gccgatgcgg cggcatgcac aaccttcgga agttgcaaca agcgtgccac
     1021 cgctggtcat tcgggaaacc aagcgaagtg gaggggaagg aagcgcgggc tgtatcgaat
     1081 ggaagacgtt tgggcgtcac gcaccgcgaa cgagcaggcg aaatcgcccg tcgttgcgaa
     1141 gggccgacgc ggtgtagcga agcggtgaca gcgtacgtcg gcgcatacgc tgctgcgcaa
     1201 gctcaaacat cctgtgcccg ggcgggtgtg tcgagcatgc gtttcatgcc ctcgtgcgaa
     1261 gcgacgaagc cgaggtcctg gtagaagcgg tgggcgtcgg tgcgcgactt gtgcgtggtg
     1321 agctgaacca tcccgcagcc gcgcgcgcgg gcacgctcga ctgcatggtg gatcagcgcg
     1381 tggcctagcc cccgaccgcg catggagccg tgcacgcgga cggattcgat gagcgcacgc
     1441 cagctgccct ggtagctcag cgaaggaata taggtcagct gcatcatggc aacgacgatg
     1501 ccgtcccgct ctgccacgat cagctcgttg ttcgggtcgg catcgatatg cgcaaatgcc
     1561 gttgcatagg actcgggaag cgggtcgctc acgcgctcgc gcgcggcgcc gagggcatca
     1621 tcggcgagca tcgcaacgat agccggcagg tcggacgcgc gggcctggcg aaaggcggct
     1681 tggtgttcca tcgtgttccc cttcatgcga tgccggaagt atagcgggcg gccgcgtgcc
     1741 cggcagaatg cttgcgtccg gccaaaagac atggagacgg cctgaaccat gggcgcttgc
     1801 tgcccctctt ccagatggag ttccggttgc gatgcgacgg catccggttt cacaacgggt
     1861 gccaggcatg atggggtatg catgatggga tacaagcacg gcagcgccgt cgttgcatcc
     1921 gcgcatgtgg gtttggcgcg ggcagcggat gacgccgacg ttgaagtcaa ggtgtacgag
     1981 aacagcattc ctccgttcgc tgagcgcgaa atggagcgac tgtacggcag cttctatgca
     2041 tcgatgcccc agctcgagat cgaaggctcg acccgcgggg tgagcacctg ggtcgcgagc
     2101 cggcatggcg aaatcatcga actgtggctg taccgcatcg aaggacgcga ggtgcgggtg
     2161 ctcaatgaag ccatcaccgt gacggccgcc gcggtgcgcc gtttcgccga tacggtgttc
     2221 gccggatcgg ctgcgttgga cgcgatctgc ttcaatgcga tctacccggc cctggacggg
     2281 ctggccctgc cctgccagcg tgtccactgc accgacgata ccgtgatcgt gctgcccagg
     2341 aacgaagaag cttggctcgc cgcgctgggc aagaccacgc gcaaaaacat gcgccgctac
     2401 atgcagcgct tcaccgaagc cttcccgggc taccgctatc gcgtctacga acgcgacgca
     2461 atcccggaag cgcatgtgcg cgcgatcatt gcgttcaacc gcgcgcgcat gactgccaag
     2521 ggcaaggtct ccggcgtcga cgccgagcag gaggcgcgca tcctgaagct ggtgcggcgc
     2581 tgcggcctga tcggcgtggc gacgatcgac gacaggatcg tcggcggcgc catcgtctac
     2641 caggtcggcg cgaactattt ctcgtacctg cgcgcgcacg atccagcata taacgactac
     2701 cgcctcggcc tgatcggcgg ctacgagctg gtgcgtacct gcatcggccg gggcggacgc
     2761 gagctgcact tgatgtgggg acgcgagccg cacaagctcc tgctcggcgg cgagcagcgc
     2821 gaactggacc gcgtcgtcgt ctaccgctcg cgcctgcgcg cgctgcgtca cctgcaccgc
     2881 atcgcacaca ccgcttgggc gagccggacg cgggcggcac gcgcctggct gaccgaactg
     2941 gcggcgcggc gcgaaggccc gatcgggcat ctgctgcaca gtgctcgcgg cgtcgtccga
     3001 cggctgcggc gctaggcgtt cggtggcggt actacggtca gatatcgacc ggatccacct
     3061 cgagcgacca gcgcgcgcgc gccttcatcg cgcgcaacag ccgcatccac tccttgagaa
     3121 aggcctgcag cgccggccgt gacgccgact cgaccagaag ttgcgcgcgc tcgacgtttg
     3181 ccacacgggt gatcgtcatc ggtatcggct catggatcac gatgcccgga tgctcgaggc
     3241 aggacgcggc gtcggtcagg aaatcgagcg ccgcctgcag ctcgcgcgct tccgcgcgca
     3301 gcagcgcctg gtacacgaac ggcggcaggc ccgccgcatg gcgctcctcc agcagtcctt
     3361 gcgcaaagcg gtcatagtcg tgcgcgatca gcgcgccata cagcggatgc tgcggatagc
     3421 gggtctgaat cagcacctcg ctggcgctgc ccccctcctt ctgcccggcc cggccggccc
     3481 ggcccgatac ctgcatcaac tgggcgaaca ggcgctcggc cgcgcggtaa tcgtgcgaga
     3541 acagcgcggt gtcgggattg agcaccccca ccagcgtcaa gttgcggaag tcatggccct
     3601 tggcgaccat ctgcgtgccg atcaggatgt cgacttcgcc gcgatgcacg ctgtcgaacg
     3661 cagcttgggc gctgcctttc ctgcgcgtgg aatcggcatc gatgcgcagg atgcgcgcct
     3721 gcggaaacag ctgctgcaga ctttcctcga tacgctgggt gccgcgtccc agcggctgca
     3781 ggtcgacgtt gccgcaggtc gggcaggaac gcgggatacg ctgctggagg ctgcaatggt
     3841 ggcagcgcag cctgtgctcg ggcttgtgca ggaccatgaa ggcggtgcag cgcgggcagt
     3901 tggaaatcca tccgcaggcg tcgcaggaaa gcaccggcgc atagccgcgc cggttcaggt
     3961 aaagcagcga ctgctcgccg cgctccaacc gcagcttgag cgcggcggcc agcgtcgcgg
     4021 tcaatccttc gcccgccttg tcgcgctcgg tgtcgatcag ccgcaccgcc gggaggaccg
     4081 ccgcgcgcga ggcccgccgg cgcagctcga ggcggcggta gcgcccgctc ccggcatggt
     4141 gccaggtctc cagcgacggc gtggccgagc cgagcacgat aggaatgccg agctggcgcg
     4201 cgcgccacac tgcgaggtcg cgtgccgaat agcgcaagcc ttcctgctgc ttgtaagacg
     4261 ggtcgtgctc ctcgtcgacg acgatcagct tcaggtgcgg cagcgacgcc aggatcgcca
     4321 gccgcgtgcc gaggatgatg cgcgcgtggc caaggtgcgc ggccagccag tggcgcaggc
     4381 gctccccttc ggccaggccg ctgtgcaggg tcgccagtgg cacgtccgga aagcgcgcgc
     4441 ggatgttctg ttcgagctgc ggcgtcaggt tgatttccgg cacaaggatc aggatctgcg
     4501 tcggctcgtc cggaggtgcc gcggcgagca ccctggctgc cgcctgcagg tagacctcgg
     4561 tcttgccgct gcctgtgacg ccgtacaaca gcatcgcggc gaaaccgcgc gcctcggcca
     4621 aggcatcgac ggcagcttgc tgctccggat tgagcaccgg cggctgcgac gggcagcagg
     4681 catcgccatg ctcgcttccc gccagctttt tcagtgcgcg gtccagcgaa acggtggtcg
     4741 cggtgcgcag gttcttcggc aacgccggca acgccacctc gccaagcgga cgctggtagt
     4801 agtcggccgc gaaggcgcac aagcccagcc aatgcggcga taccggcggc agttggcgcc
     4861 ggaccgccat cacatccttg agcttatcga cgggcagcgt gctcgcgtcg ccgacgcgga
     4921 cgaccagccc cacgctctcg cggcggccaa acggcaccag gaccagctgg ccagcctgcg
     4981 gctcgggctc tccgtcctcg ccgcaccagc ggtagtcaaa caaggtatcg agcggcgtat
     5041 cgagcgcgac ttgaagaatg cgatgttcca cgacttaatg cgaatcttcg gagtgtgttc
     5101 taaggccgcg attccaaagg actttttccc ctatccacaa aagctgtgga taactttgtg
     5161 gagaaatgcg gcgtacccat tggaaacgct cgatttcatg cgggtttcga cagattgccc
     5221 gtttgaaaag caaattttcg ccccttttaa atcaagcact tagctgcatc gattgggatg
     5281 ccacaaagtt ggtgtgagaa attgtttccc gcgctcatgg cgaatcagca agtatttcgc
     5341 tgtgaacaag tgggatccca cccttgcctt cgcctggcaa ttccttccgc accgcctcgg
     5401 aaacttcaag tgaatcctca aaatgtctca caagtgcccg attttgcagc ggctttttca
     5461 agggcatgcg agcttgtgga taactttgtg aacatccgcc gagcaactgc ggctgccgtc
     5521 gacatggtct cgcagcaggg cagcgctctg gcgcattgac gctggataaa tccgtgaatt
     5581 ttcaatgact tagatggtct gccggggtgc cgccccacgc gcccccccga gcagtgccat
     5641 tgcgccgcac aactgcggtt ctgtgaataa agagagggtg acggtccgat tctgtacaac
     5701 aagagagtca ggccggcgca gcactttccg ctcgcagcgc acgcccggcc gtgtggactt
     5761 cctcggccag caccgacacc gcatccggcg gcgtgaactg cgaaatgcca tggccaaggt
     5821 tgaacacgtg accatggccg ggcgcgccgt atgaagccag cagcttgcgc acctccgcgc
     5881 ggatctgctc ggggccggca aacagcacat tcgggtcgag gttgccctgt agcgcgacct
     5941 tgtcgcccac gcgcgccttt gcgcgtccaa gattgaccgt ccagtccagg ccgacagcat
     6001 ccgtgccgat ggcggcgatt tcttccagcc acagcccgcc gcccttcgtg aatacgatca
     6061 ccgggatgcg cgcgccgtca tgctcgcgct tgaggcgggc aagcacgtcg cgcatgtagg
     6121 ccagcgagaa ttgctggtag atgccgtcgg ccagcacgcc gccccaggta tcgaagatca
     6181 tcaccgcctg ggccccggcc tcgatctggg cattcagata atcgccaacg gcctcggcat
     6241 tcgtcttcag gatgtggtgc atcaggtccg ggcgctgata cagcatggtc ttgacgcggc
     6301 ggaagtcgtc cgatccgcca ccctcgacca tgtagcaggc cagcgtccac gggctgccgg
     6361 aaaagccgat cagcggtacg cggccaccca gctcggtgcg gatctgcgtg accgcgtcga
     6421 acacgtactg caggctgccc ggctcgggcg tcttcaacgc gagcactgcc tgctcatcgc
     6481 gcagcgggcg ctcgaacttc ggcccctcgc cttcggcgaa gtacagcccc aggcccatcg
     6541 cgtcgggcac ggtcaggatg tcggaaaaca ggattgccgc atcgagcgcg taccggtcca
     6601 gcggctgcag cgtgacttcg gtggcaaagt ccgggttctt cgcaaggccg aggaaggagc
     6661 cggcgcgctt gcgtgttgca caatattcgg gcaggtagcg cccggcctgc cgcatcagcc
     6721 acattggcgt gtagtcggtc ggctcgcgca gcagcgcgcg caggaaggtg tcgtttttca
     6781 gcgggggaaa gttgttcggc atggtcggct ctcgatcaat ccgacattat cgccgatggc
     6841 gccgactcag tccctgcacc ggcggcttga catcgcgcgc cgatcatcct gatcccgcaa
     6901 aacactagcc gagcagcttg gtcgactgct cctcgtctgc gacgcgacgg atataggcgg
     6961 cgaaatccgg atcctccccg cgcagcagat gtggcagcag gaaatagaag tcgtcgcgcc
     7021 ggcaccattc gcgcatgtag tcgtcccacg aattccagcg gcgcagcgcc acccccgaga
     7081 ggttgtcatc ataggaaacg atgtacgcgc gctcgaacag cgagatcagc atctcgaaaa
     7141 tgatcaaggc gcgctcgcgc tgctcatccg tcatgtcggc ccgcatcgtg gtgctgcgca
     7201 gctgcagatc aggattggca agcacgattt cgaggaattc gttgtaggcg ttctgcaaga
     7261 gctggtaggc ctcctcgtcc tcgttgtcac gctccttgcg ctgctcgtag aagaacagaa
     7321 agatcgcgaa cggcaggccg acgaccgtca cgacatagct ggcggtttcc cagaaggtgg
     7381 gattgaaaag aaattccatg attgttcttg taggcgcccg gtaggggcga ttgcaaagct
     7441 accacgcgcg cacgcgcgcc ggccagctag aagccccaac aaaaggcggc tggcggcgtt
     7501 gcgccccctt gccgtactac acgtactgtc tgcggcggcg tgccttgcca tcggcatttt
     7561 gtttgggctt cttagtcaga gtggccgaag gcggcggcgc atgaatcggc caggacggcg
     7621 gcccatgcgc tgcagccggc agccgacgag tggtaaccgt cgtgcgccat caggccggga
     7681 ccgtcgaggc cggacggcag cggaacgtgc agcgtattgg cgcgggtccg caccagtgcg
     7741 cgcagcacgc gatccaggct ggcggacttc aggcccatca ccgcccgcag cggctgcggc
     7801 agcgccggaa aacgccccat cggcggcacg cccgacacca gcatcaggcg cggcgcacag
     7861 cgcgcctcca gcgcgtcgag cagatccctg acgtcctggc gccactgccc ggcgctgcga
     7921 aacgccgtcg tatcgttcac gccgaatgca acgaggacca ggtcctgcgg ttgcgccgcc
     7981 acgtgcggca gcaggcttcc catcgcgtcg cgcaccgtgg cgccgttctg gccacgcgcc
     8041 tcccagtgga ccggacgcct caagcgcgcg gccagagcct gcgcgaggca tccggtaatc
     8101 gcctcctcgt gcgactgcac acccaccccc gccaccggcg actcgccgaa tgccagcatg
     8161 cgcagcggcg cggcgttgaa gtggcgtccg gcgatcccgt cagtcgcgcc cggcgcctcc
     8221 ggcaggcgcg gcgtgatgcg gcgcgtacgg cggccctgcg cgagcaacag cggcagcaag
     8281 ggcagcgcga ccagttccgg caggtagtcg cgcatcgccg ttcagcgggc ggcgaccagc
     8341 gccgcgtgtt cgatcttgat gcacttgtcc atcacgacct ccagcccggc tgcgcgcgcc
     8401 ctctccgctg caaccgcatc ctctatgcca agctgcatcc aaacgctgcc ggcgcggatc
     8461 gcgatcgcct cgtccaccac cgcagcgatg tgctcggggc gccggaagca gtccacgata
     8521 tcgatcttcc ccgcctccgc cgacacgacc tgcgccgcca gggtgagcgt ggcgtagcaa
     8581 tgctcaccga gaatgtgagc gcccgcatac gttggattca ccggaatgat ccggtacccc
     8641 tgcgcctgca tgtaggcggc gacttcataa ctggggcgat ccgggtgggc ggacagtccg
     8701 acgaccgcaa tcgtgcggga gtccttgagc aggcgggaga tgtcggtggg tacggtcatg
     8761 ttgctccaaa aattgagtgc ttaagctgga atgccattcg ctcaagcgct tgcccaagtc
     8821 ggttgcgccc gggcggcgcc cgccctttcg gcggaacgga aattccaggg cggcggacct
     8881 gcttcagggc gctcgacggg cgaagacaat tcggggccag ttgcgtgcgc cggcagcatt
     8941 tcagccgagg cttcccgtgg attttagcct ttccagcttg ccaaggtatt gcagcaatct
     9001 tgacgctgcg gccccgtcag gagaggtcat ccgtttatta gccgggtgac aatcacgctt
     9061 ccgtgcgtgt tatctttttc gcatttttgg cgggcgatca cgccaggccc gacgctgcgt
     9121 cccactacca tttcgattgg atgaggattt tgtccgtgag ccccctttgc ctgcctcgag
     9181 cggcgcacaa gcgcctgccg tttgcatttc atgtgctgct gctggcgctg cttcagccgc
     9241 cactcagtca agcaaaccaa ctgaacatcc tgcccagcga gatcgtcaag gccgaaacgc
     9301 gttggcgctt ctccgagaac gcgacgctgg atgaagagca atgggttcag cgtcgcgcgc
     9361 tcagcgatga gggcgcggtg cagatcggcc gccagcgctt ttcattcaat accgagctgg
     9421 aggatttcag cattctcgag gcatacacgc tcaaggcgga tggccggaag ctgctggtac
     9481 acccggatga ggtgctggtg cagcgtggcc tggcagtcgg agggacagcc gtaagtttgc
     9541 ctagcgtgga gatgcatcag ctgacctatc ccgacgtgca gaaaggcgac agcgtcgtct
     9601 ggcgctaccg gaaaaaaagc atgcggaccg cgctgccggg ctgggccagt gcggccaggt
     9661 ttgcctatcc atgggagagc gtactccagg agcaggtacg catcgagtct ccgcaagcac
     9721 tgaggctgca tgtctttgcc gccggttacg acgctcgcaa gaaccaggag gacggtttgg
     9781 acgtctggat catcgaggga aagcaccagg ctttcccgat tgagcagaac tgggctgact
     9841 cgctgcagtg gctgcccagg gtgtatgcca caaccctgcc ggatcatgag gcctgggccc
     9901 tgcgattcgc cgagcctatc cggcagaaaa gcgaaaccac gccggctgtc cgcgcactgg
     9961 cgcagcggat taccaagggc catgctacca gccacgagaa ggcgcgcgcg gtgtacgact
    10021 ggatccgttc gaatatccgc tacgtcgctg tgcatgccgg ggtcgggggc tacgttcccc
    10081 atgaggtgga agacatcctc cgcaaacgct atggcgattg caaggaccag actctattga
    10141 tgattgccct gctcaaggcg gtcggcatcg aggccgcgcc cgcgttgctg tacaccgccc
    10201 ccgaatacgc gctccctgag ctgcccgtcg gcactgacca tgtgatcgtc tacattccgg
    10261 aactcgactt gtttgccgat gctacggcga gcacaatccc gttcggttcg cttccctgga
    10321 acgccgccga caagccggcc gtcgtcgcga tggccgaagg ctcgcaccgc atgcgcacgc
    10381 cggcgtttcg cgcagacgac aaccgtctga tagtcaaatc cgtatggcag attgcccgtg
    10441 atggatcggc cgatgcggtg atcgaggtgg aaggctcggg caacgcagcg acgctgcttc
    10501 aggatcggct gcagcaaatc cccgcaggca tgggaagcgt cgcggtgcag cgcattcttg
    10561 aaacgagcag gcttagtgga cgcggcttca tccattttcc agccgtgcag cgcgagcgcc
    10621 agcagcaatc gatgcgattg gacctggaga atgtgcgcaa cctgatcgtc gatccagcag
    10681 caggcagcct cgcgccccac cctgcattga atctgcccct gtacatcctg agcaacatgg
    10741 ggaactacgc ggcggaggcc cggcgccatc cgatgacctg cacgcctgtg cgccttcgtg
    10801 aggacttcga gctcaacctg gatccaggct tcgatatact tcgcatcccc aaggacgcgc
    10861 gggaggagca cgccgacggg atcgtctttg aagcgcgata taccgtgaac ggcaatcggc
    10921 tgagcggatg gcgagagctc atccttgacc atggacgcca ttcttgcaat gtcgaccagt
    10981 acaaggaacg ccgcgcggtg atgcaacgca tcgatcagca cttgcgcggc agcgtgctct
    11041 atacgcaata gcgcacgccg gcgacggctg caggttcaga ggcgcggcgg ttcacgtccg
    11101 cgccatgaaa aaaaggcagc cgaagctgcc tttcgatcgt agcctttccg gcttgccaac
    11161 gcgttctagc gcttttgacg cagcttctga atcgcagcca attgcgcgac cgccgcggcc
    11221 agttcagcct gcgcgcgcgc gtagtcgatc gacgattcgt tgttcgccat cgcttcctcg
    11281 gccagcttct tcgcttcgct cgccttcgcc tcgtccagat cgtgaccgcg gatcgcggta
    11341 tcggccagga ccgtcacggc gttcggttgc acttcgagga tgccgcccgc gacgaacacg
    11401 aactcgtctt cttcctggcc cggcaccttg atgcgtaccg cacccggacg gatgcgcgtg
    11461 atcagcggcg tgtgcttcgg gtagatgccc agctcgcccg cttcacccgg caacgcgacg
    11521 aactcggctt cgccggcgaa gatttcttct tcggcggaga cgacgtctac gtggatagtg
    11581 tttgccatga tggaaccttt ttgttcgctt gaagtttcag ggcaggccgg caatccggcc
    11641 taccgagcct gatacggttc cccgcgccgc cggcgcaagg aaccgaagcg ctcttactga
    11701 agc
//
LOCUS       DATLGF010000511         5402 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_203994, whole genome shotgun sequence.
ACCESSION   DATLGF010000511 DATLGF010000000
VERSION     DATLGF010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5402
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203994"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1920)
                     /gene="mrdA"
                     /locus_tag="VM406_15935"
     CDS             complement(<1..1920)
                     /gene="mrdA"
                     /locus_tag="VM406_15935"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HVL77503.1"
                     /translation="MTEFKNTEKELHSFRMRLSIAAVLVLICFGLLFARFTWLQMFKY
                     EDYADRAENNRISVLPVPPNRGLIVDRNGVVLARNFSAYTLEITPSKLRQPLDAVIDE
                     LATIVEIQPRDRRRFRRLLEESKRFESVPIRTRLSDEEVARFASQRYRFPGVDIQARL
                     FRQYPHGATASHVIGYIGRISQRDAERISKSDDAANYIGTDYIGKEGVEKRYESVLHG
                     TTGWEEVEVTAGGRAVRTLSRTASSPGHNLILSIDIELQKIIEEAFGDRRGALVAIEP
                     ATGDVLAYVSMPTYDPNLFVEGIDQQSWDFLNNSIDRPLLNRPLIGTYPPGSTYKPFV
                     ALAGLELGKRTPKSAIADIGFFMFGNHRFSDNKPGGHGMVDLYKSLVVSCNTYYYQLA
                     NDLGVDAMHDFMKPFGFGQRTGIDLDNERVGVLPSTHWKRTAYKRPEQQRWYAGETIS
                     VGIGQGYNSFTPLQLAHATANLANNGVVMRPHLVKIIEHGVTKERELTVPKESYRIPL
                     KQENIDAVKRGLVGVTTEGTGARLFANAPYTSAGKTGTAQAVGLRRGEKYDAKKMAEH
                     LRDHSLYMAFAPAENPRIALAVIVENAGFGSAAAAPIAKIAFDYFLLGKRPGDPPTPA
                     AAQGAGARPAAPAPQA"
     gene            complement(1940..2452)
                     /gene="mreD"
                     /locus_tag="VM406_15940"
     CDS             complement(1940..2452)
                     /gene="mreD"
                     /locus_tag="VM406_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HVL77504.1"
                     /translation="MNRPHYILLPVNPLFITGSLVVAFMLNLLPWGHWYGVPDFVALV
                     LVFWSIHQPRRVGIGIAFLMGLLMDVHDATLLGENALSYTLLSYFAIMVHRRMLWFRL
                     LGQALHVLPLLLMMQTVQLVVRVAVTGNFPGWLYFSQSLVAAAIWPIVTMILLAPQRR
                     AINRDETRPI"
     gene            complement(2452..3447)
                     /gene="mreC"
                     /locus_tag="VM406_15945"
     CDS             complement(2452..3447)
                     /gene="mreC"
                     /locus_tag="VM406_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HVL77505.1"
                     /translation="MEYSPPPLFKQGASARAKVVIFSLIALILLVADSRMRSLGAIRQ
                     AVGTVLYPMQVVALLPRDAAYAVAGYFSTVSLLQNENQQMRAARTADAIQLQQVQQLL
                     AENAHLRGLLEASERLEVNTIMTEILYDARDAFTRKIVVDRGTQHGVATGQPVIDESG
                     VVGQVTRVFPFTSEVTLLTDRDQAIPVQVVRSGLRSIAYGQGRSGALDLRFMPVNADI
                     QNGDILVTSGLDGVYPPGLAVAKVTKVETRSNDAFARIVCQPLAGIDRNRQLLILLTS
                     SEQFPSRPEPEGQTRERAARQRPAHSVPPATNPPQQTPAPLPAATPPSTPTPGAT"
     gene            complement(3609..4652)
                     /locus_tag="VM406_15950"
     CDS             complement(3609..4652)
                     /locus_tag="VM406_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HVL77506.1"
                     /translation="MFGFLRSYFSNDLAIDLGTANTLIYVRNMGIVLDEPSVVAIRQQ
                     GGPNGKKTIQAVGKEAKQMLGKVPGNIEAIRPMKDGVIADFTVTEQMLKQFIRMVHDS
                     KLFKPSPRIIICVPCGSTQVERRAIRESALGAGASQVYLIEEPMAAAIGAGLPVSDAT
                     GSMVVDIGGGTTEVGIISLGGMVYKGSVRVGGDKFDEAIVNYIRRNYGMLIGEQTAEL
                     IKKEIGSAFPGSEIKEMEVKGRNLSEGIPRSFTISSNEILEALTDPLNNIVSAVKNAL
                     EQTPPELGADIAEKGMMLTGGGALLRDLDRLLMEETGLPVIVAEDPLTCVVRGSGMAL
                     ERMDKLGSIFSYE"
     gene            4905..5204
                     /gene="gatC"
                     /locus_tag="VM406_15955"
     CDS             4905..5204
                     /gene="gatC"
                     /locus_tag="VM406_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HVL77507.1"
                     /translation="MSLNLSDVKRLARLAQLDLNDEQGEKTLAKLNGIFDLVEQMRAV
                     DTAGVEPLSHPIAAYQDVSLRLREDAVTEPDRRDDYQKVAPATEDGLYLVPKVIE"
     gene            5214..>5402
                     /locus_tag="VM406_15960"
     CDS             5214..>5402
                     /locus_tag="VM406_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit A"
                     /protein_id="HVL77508.1"
                     /translation="MHKHTLKQLSAQLQARQVSASELAQHYLKRIEQSDLNAFLHVDA
                     ELTMEQAAEADRRIAAGDT"
BASE COUNT          948 a         1716 c         1774 g          964 t
ORIGIN      
        1 cgcctgcggg gccggcgctg ccggacgtgc gccggccccc tgcgccgcag ccggcgtcgg
       61 cggatcgccc gggcgcttgc caagcaggaa atagtcgaat gcgatcttcg cgatcggcgc
      121 agccgcggcc gaaccgaagc cggcgttctc cacgatcacc gccagcgcga tgcgcgggtt
      181 ttcggccggc gcgaaggcca tgtagagcga gtggtcgcgc agatgctcgg ccatcttttt
      241 cgcatcgtac ttctcgccgc ggcgaaggcc caccgcctgc gcggtgccgg tcttgccggc
      301 cgaggtgtac ggcgcgttcg cgaacagccg cgcgccggtg ccttcggtgg tcacgccgac
      361 cagcccgcgc ttgaccgcgt cgatgttctc ctgcttgagc ggaatgcggt agctctcctt
      421 cggcacggtc agctcgcgct ccttcgtcac gccatgctcg atgatcttca ccaggtgcgg
      481 ccgcatcacc acgccgttgt tggcgaggtt ggcggtcgcg tgcgccagct gcagcggggt
      541 aaacgagttg tatccctggc cgataccgac cgagatcgtc tcgccggcat accagcgctg
      601 ctgctcgggc cgcttgtagg cggtacgctt ccagtgggtg gagggcagca cgccgacgcg
      661 ctcgttgtcc agatcgatgc ccgtgcgctg gccgaagccg aacggtttca tgaagtcgtg
      721 catggcatcg acaccaaggt cgttggccag ctggtagtag taggtattgc aggagacgac
      781 cagcgacttg tacaggtcca ccatgccgtg gccgcccggc ttgttgtcgg agaaccggtg
      841 attgccgaac atgaagaagc caatgtcggc gatcgccgat ttcggcgtgc gcttgcccag
      901 ttcgagaccg gcgagcgcga cgaagggctt gtaggtcgag ccgggcgggt aggtgccgat
      961 cagcggacgg ttcaacagcg gccggtcgat cgaattgttg aggaagtccc agctctgctg
     1021 gtcgatgcct tcaacgaaca ggttcgggtc gtaggtcggc atcgagacgt aggcgagcac
     1081 gtcgccggta gctggctcga tcgcgaccag tgcgccgcga cggtcgccga atgcttcctc
     1141 gatgatcttc tgcagctcga tgtcgatcga gagaatcagg ttgtgaccgg gcgacgatgc
     1201 ggtacgcgac agcgtgcgca ccgcgcgtcc gcctgccgtc acctcgacct cttcccagcc
     1261 ggtggtgcca tgcaggacgc tttcatagcg cttctccacg ccttccttgc cgatgtagtc
     1321 ggtgccgatg taattggccg catcgtcgga cttgctgatg cgctcggcgt cgcgctggct
     1381 gatgcggccg atgtagccta tcacatgcga cgcggtcgcg ccatgcgggt actggcggaa
     1441 caggcgggcc tggatgtcca cgccggggaa gcggtagcgc tgcgacgcga aacgcgcgac
     1501 ttcctcgtcc gacaggcggg tgcggatcgg cacgctttcg aagcgcttcg attcctccag
     1561 caggcggcgg aagcgacgcc tgtcgcgcgg ctggatctcg acgatggtgg ccagttcgtc
     1621 aatcactgca tcgagcggct ggcgcagctt cgaaggcgtg atttccagcg tataggcgga
     1681 gaagttgcgc gcgaggacca cgccgttgcg gtcgacgatc aggccgcggt tcggcggtac
     1741 cggcagtacc gagatgcggt tgttctcggc ccggtccgcg taatcctcgt acttgaacat
     1801 ctgcagccag gtgaaacgcg caaacagcag tccgaagcag atcagcacga gcaccgcggc
     1861 gatcgacaag cgcatgcgga agctgtgcag ttccttttcg gtgttcttga actcggtcat
     1921 ggaagtcgga cgtcgcggtt cagatcggcc gtgtctcgtc ccggttgatc gcgcgccgct
     1981 gcggggcgag caggatcatc gtgacgatcg gccagatcgc ggcggcgacc aggctttgcg
     2041 agaagtacag ccagccgggg aagttgccgg tcaccgccac gcgcacgacc agctggaccg
     2101 tctgcatcat caacagcagc ggcagtacat gcagcgcctg gccgagcagg cggaaccaca
     2161 gcatgcgccg atggaccatg atcgcgaagt aggacagcag cgtatacgac agcgcgttct
     2221 cgccgagcag cgtggcgtcg tgcacgtcca tcaggagacc catcaggaag gcgatcccga
     2281 tgcccacgcg gcgcggctgg tgaatgctcc agaacaccag gacgagggcg acgaagtccg
     2341 gcacgccgta ccagtgcccc cagggcagga ggttcagcat gaacgcgacg accaggctgc
     2401 cggtgatgaa cagcggattg accggcagca ggatgtagtg ggggcggttc atcacgtcgc
     2461 tcctggcgtc ggcgtcgaag ggggcgtcgc ggcgggcagc ggggccggag tttgctgcgg
     2521 cggattggtt gcgggcggca cgctgtgcgc cggtcgctgg cgggctgcgc gctcgcgcgt
     2581 ctgcccctcc ggctcgggac gcgacgggaa ttgttcggac gatgtgagca ggatcagcag
     2641 ctgccggttg cggtcgatcc cggccagcgg ctggcagacg atgcgggcga aggcatcgtt
     2701 ggaacgggtt tcgaccttgg tcaccttggc cacagccagc cccggaggat agacgccgtc
     2761 gaggccggaa gtcaccagga tgtcgccatt ctggatgtcg gcgttgaccg gcatgaagcg
     2821 cagatcgagc gcgccggagc ggccctggcc gtaggcgatg ctgcgcaggc cgctgcgcac
     2881 gacctgcacc ggaattgcct ggtcgcggtc ggtcagcagc gtcacttcgg acgtgaacgg
     2941 gaaaacacgg gtgacctggc cgaccacccc gctttcgtcg atgaccggct gccccgttgc
     3001 gacgccgtgc tgcgtgccgc gatcgaccac gatcttgcgc gtgaacgcgt cgcgggcgtc
     3061 gtacaggatc tcggtcatga tcgtattgac ttccagccgc tcgctggctt cgagcagccc
     3121 gcgcaggtgg gcgttctcgg ccagaagctg ctgcacctgc tgaagctgta tcgcgtcggc
     3181 ggtacgcgcc gcgcgcatct gctggttttc gttctgcagc aaggagacgg tggagaagta
     3241 gccggcgacg gcataggcgg cgtcgcgcgg cagcagcgca acgacctgca tcgggtagag
     3301 gacggtgccg actgcctggc ggattgcgcc aagcgagcgc atgcgcgaat cggcgaccag
     3361 caggatcagg gcgatcagcg agaagatcac cactttcgcg cgggcggagg cgccctgttt
     3421 gaaaagcggc ggagggctgt attccattta ctgcctgttc aagaaatggc gttgacccgc
     3481 tccatgacct cggagccagt gtcgttcaat gaaaattcct caggacgccg gctcgcgccg
     3541 gcacacgctg cccgctcatc ccatttttca agatgccgcc cgaacgaata cgcgcctgcc
     3601 tgtgccgctt attcgtacga gaagatcgaa cccagcttgt ccatgcgctc gagcgccatg
     3661 ccggagccgc gcaccacaca ggtcagcgga tcctcggcga cgatcaccgg caggccggtt
     3721 tcttccatca ggaggcggtc aaggtcgcgc agcagcgcgc cgccgccggt gagcatcatg
     3781 cccttctcgg cgatgtcggc gcccagttca ggcggggtct gctcgagcgc gttcttgacg
     3841 gcggagacga tgttgttcag cgggtcggtc agcgcttcaa ggatctcgtt ggacgagatc
     3901 gtgaacgagc gcgggatgcc ttcggacagg ttgcggccct tgacttccat ctccttgatc
     3961 tcggagccgg ggaaggcgga gccgatttcc ttcttgatca gttcggcggt ctgttcgccg
     4021 atcagcatgc cgtagttgcg gcggatgtag ttgacgatgg cttcatcgaa cttgtcgccg
     4081 ccgacgcgca ccgaaccctt gtacaccatg ccgccgagcg aaatgatgcc cacttcggtg
     4141 gtgccgccgc cgatgtcgac caccatcgag ccggtggcgt ccgagaccgg caggccggca
     4201 ccgatcgcgg ccgccatcgg ttcttcgatc aaataaacct gggaggcgcc ggcgccgagc
     4261 gccgattcgc ggatcgcgcg gcgttcgacc tgggtcgaac cgcagggcac gcagatgatg
     4321 atgcgggggg acggcttgaa cagcttggag tcgtgcacca tgcggatgaa ctgcttcagc
     4381 atctgctctg tgacggtgaa gtcggcgatc acgccatcct tcatcgggcg gatcgcctcg
     4441 atgttgccgg gcaccttgcc cagcatctgc ttggcttcct tgccgacggc ctggatcgtc
     4501 ttcttgccgt tggggccgcc ctgctggcgg atcgccacca ccgagggttc gtccaacacg
     4561 atgcccatgt tgcgcacata gatcagcgtg ttcgccgtac ccaggtcgat cgccaggtca
     4621 ttcgaaaaat aactgcgtag aaatccaaac atgtatcgtc ccgattgcgc cgccccggcg
     4681 cactttgaaa taggtggtga acggtctcgc gccccttgcc tgcaggcctt gcagacccgg
     4741 ctggcagccg cacattctac cttataatct cgccgagatt agccgacgga aacctcagaa
     4801 ataagggctg aacgacccgt ttttgcaagc atttggcagc acaagaatgc ttttggcaaa
     4861 cccgcatccg cgcgtccgaa cccttgtcca tttcactact cgacatgtca ctcaacctct
     4921 ccgatgtgaa gcgcctggcc cgcctcgcac agctcgacct gaacgatgaa caaggcgaga
     4981 aaacgctcgc caagctcaat ggcatcttcg acctcgtcga gcagatgcgc gccgtcgata
     5041 ctgccggcgt ggaaccgctg tcccatccga tcgccgctta ccaggacgtc tcgctgcgcc
     5101 tgcgcgaaga cgccgtcacc gaaccggacc gccgcgacga ctaccagaag gtcgcccccg
     5161 ccaccgagga cgggctgtac ctggtcccga aggtcatcga ataacgcatc gccatgcaca
     5221 agcacaccct caagcaactc tccgcccaat tgcaggccag acaggtctcg gccagcgaac
     5281 tggcccagca ttatctgaag cgcatcgaac agagcgacct caacgccttc ctgcacgtcg
     5341 acgccgaact gaccatggag caggccgccg aggccgaccg ccgcatcgcc gccggcgaca
     5401 cc
//
LOCUS       DATLGF010000512         9277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_157859, whole genome shotgun sequence.
ACCESSION   DATLGF010000512 DATLGF010000000
VERSION     DATLGF010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 9277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9277
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157859"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..159
                     /locus_tag="VM406_15965"
     CDS             <1..159
                     /locus_tag="VM406_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HVL77509.1"
                     /translation="QISGGMLPKISSALDAAKSGVNTVHIIDGRIEHSLLLEVLTEQA
                     FGTMIRSH"
     gene            complement(272..1204)
                     /locus_tag="VM406_15970"
     CDS             complement(272..1204)
                     /locus_tag="VM406_15970"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HVL77510.1"
                     /translation="MKLWMTALLSAALLAPAHAGNDAAAAVTIQAGRDASVAAPTRGA
                     LYRVRHEGTVSYLFGTIHVGTPAHMVLDAEVERALMQSRRLVLELDVRDDAPVHRALE
                     KHGLYSPGTGIDRHLDADTLRLLKDALERFGLAYEAVQGMKPWLVANLLQGLELDRNG
                     FRRHYGAEYNLIAFAPGHAVLELETAEYQMALFDGMSAAAQEEYLRETLQDLQAGRAL
                     RKARELIDAWASADRDTLARLWQESLTEGSRTAEFTHRVLLDERNPLMADKVEILLKE
                     RESSFVAVGLLHLIGKNGLPALLRQRGYSVERVY"
     gene            1251..1988
                     /locus_tag="VM406_15975"
     CDS             1251..1988
                     /locus_tag="VM406_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HVL77511.1"
                     /translation="MTRPIWLFDLDNTLHNASQAVFPAINANMNAYMARVLGSDGTPA
                     APDVVNAMRLAYWKRYGATLLGMVKYHQVKMEEFLIEAHRFDDLPAMLRAERGLARLL
                     KRLPGRKVLLTNAPRRYSREVLHHFGLHRHFARHIPIEAMHVHRQLRPKPSRHLLRKL
                     MRREGAAAHRCILVEDTLDNLKAAKMLGMRTVWVTQYLASNPHLRPVPADRPLPAFSK
                     RPVYVDVKVKSVTQLPAALHRLRIRTR"
     gene            1993..2562
                     /gene="slmA"
                     /locus_tag="VM406_15980"
     CDS             1993..2562
                     /gene="slmA"
                     /locus_tag="VM406_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HVL77512.1"
                     /translation="MATTKPGERKLQILQMLAQMLEQPKGEKVTTAALAARLDVSEAA
                     LYRHFASKAQMFEGLIEFIESTVFGLINQINEQQESGLAQAQAMAGMLLGFAERNPGM
                     TRVLIGDALVNEDERLQARMNQFVERVEMAFRQSLRLAETQGDADEVDAGARASMITS
                     FVVGRWHRYAKSGFRQKPTEQAQIGFLMR"
     gene            2913..3791
                     /locus_tag="VM406_15985"
     CDS             2913..3791
                     /locus_tag="VM406_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVL77513.1"
                     /translation="MSSAKPFPVFAFATLLIGGMAIGFAGIFMRLSDVNPLASAFWRM
                     ALAAPILWAWAFAVRRQDAAAGKRTDFSKALVLAGVHFAADMALWHLSLHYTTVANAT
                     LLSNFAPIFIALWLWLAYRVKFARIFIIGMVLALVGAALLVGPNAAGGPDKLLGDALG
                     LSSAIFYAAYQLVIKDARNDYSTARLMAWSTTVTGLALLPFALLAPGPFFPAALIGWL
                     PLLGLALVAQIGGQTVIAYAFAHLPASLSSVSLLIQPLTAAVAAWLIFQEAILPVQML
                     GGALLLWGIYLAKKGS"
     gene            3877..5685
                     /gene="pabB"
                     /locus_tag="VM406_15990"
     CDS             3877..5685
                     /gene="pabB"
                     /locus_tag="VM406_15990"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HVL77514.1"
                     /translation="MSPDACFALLDDSSAETRRSRLYTGLVRTLACYTARSLPALLDD
                     MQHELAAGRFAVGLFEYELGARMHGVLPDDAAASPLAQILLFSSCQLLDATETDQWLR
                     AHDDGAPAGVAGIRASVDAQAFSDAISRIHAWIEAGDTYQINYTFRLHFDAYGTPLAL
                     YRRLRARQPVPYGALIALPDGKAVLSLSPELFVRHDGGVLTARPMKGTAPACGDANED
                     ARRAAALAADPKNRAENLMIVDLLRNDLGRVAEFGSVRVPQLFQVERFASVLQMTSTV
                     EARLQQGTTLAQIFAALYPCGSITGAPKRRTMQIINALESEPRGIYTGAIGWFEAPED
                     GLATGDFCLSVPIRTLVLDAPAQGVRRGRLGVGAGIVHDSVAADEYEECRLKARFLTG
                     LPASFALIETILVQARRCRHLDLHLARLRESAAYFGFAFDALRIRDAIDAACAELPAD
                     GAFRARLCVEHDGACEVQTGALAPLDVPVKLVLANEKVDSSDLFLRHKTTQRSRYDAA
                     WREAERQGAFDMLFFNERGELTEGARSNVFVRLGGRWYTPPLSAGVLPGVMRSVLLAD
                     PAWNASERTLTRADLGQAEEIIVCNALRGALPGTLV"
     gene            complement(5665..6567)
                     /locus_tag="VM406_15995"
     CDS             complement(5665..6567)
                     /locus_tag="VM406_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVL77515.1"
                     /translation="MSAADVVKLVLLSAIWGGSFIFLRVAVPEFGPVLTAFLRTALAA
                     AVLYGFARATGVRLHWRENVKPYAAVGLCAVAIPFCAFSFAALHLPAAHMAVLNSTAP
                     LFGALLSVIWLSERLTLRKLAGLMLGVVGVAILVGAGALELGVSTLLASAACLVGSSA
                     YAVSSIVVKKTGRPGGIHPIAMAAASLIAGGIILAPAAPLAVPAELPSALAFACVLGM
                     AVLSSGLAQALFIPLIVKVGPTRAMSASFLIPLFSMLWGALFLDERVGPSTLVGAMVV
                     LLAMALVLTAPRSEAVAVLDERTR"
     gene            complement(6654..6851)
                     /locus_tag="VM406_16000"
     CDS             complement(6654..6851)
                     /locus_tag="VM406_16000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77516.1"
                     /translation="MALASSIDVLLRAMRILRATTQGARKQQPGCRGARRASPFDLHL
                     VFSCCDYSTRREWTGYVVSTG"
     gene            complement(6981..7733)
                     /gene="moeB"
                     /locus_tag="VM406_16005"
     CDS             complement(6981..7733)
                     /gene="moeB"
                     /locus_tag="VM406_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HVL77517.1"
                     /translation="MNDNQLLRYSRHILLDEIGIEGQERLLAAHALVIGAGGLGSPAA
                     YYLASAGIGKITLVDHDSVDLTNLQRQILHTTERVGQPKAQSGKQTLAQINPEVEVEA
                     LIERADAKRLGELVAGASVVLDCSDNFDTRHAVNRACVSHRVPLVSGAAIRFDAQVSV
                     FDVRQPDAPCYACLFPPDQAFEEVQCSTMGVFAPLVGIIGATQAAEALKIVAGVGESL
                     AGRLLLLDARSMEWTGIRVMRNDGCPVCSASR"
     gene            7826..8548
                     /locus_tag="VM406_16010"
     CDS             7826..8548
                     /locus_tag="VM406_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77518.1"
                     /translation="MVQRDSPRHLRALLLGPGLVIFLWALWAFIAFVLFDRDAHGLVI
                     VGVTLVLFFIVVLLLRRRGWADWRTGLYAGLFIGLLVLMYMIPIRGALPPDAHALNQR
                     ANAAHADRYDYAASIFDEVVARYVAPTREYILQPHRIFLVKDTAYFWNAQGGYVPSHI
                     QAQLYRKLLLASGRFAEQEVELRTGGCVNSPHGYVVIHHPEREIFADLWAADQFEEYR
                     FGQRVQMPGCDGVVAEPEGEPA"
     gene            8627..>9277
                     /locus_tag="VM406_16015"
     CDS             8627..>9277
                     /locus_tag="VM406_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77519.1"
                     /translation="MPANKKTTRLLAPVLLCAAIALAACGGGGHEDGHHDDAYHIDTA
                     GRLVIAADAAASVRVYDLDSSQVHATHTLANPASALYTSPGGRYAVVMQQNQDAVQFV
                     DGGIWQEDHGDHMHDYKAASNMMAWTLAGPRPSHFDVQHGKQAAIFMDGRGDAVPPQY
                     STVRLITDASIAGGSVTAGLDLDYAVHGLAEPRDDVLLVAHRATDAPDALPTHLNVY"
BASE COUNT         1549 a         3133 c         3013 g         1582 t
ORIGIN      
        1 cagatctccg gcggcatgct gccgaagatc tcgtcggcgc tggacgcggc caagtcgggt
       61 gtcaacaccg tgcatatcat tgacggccgc atcgagcatt cgctgctgct ggaagtgctg
      121 accgagcagg cgttcgggac gatgatccgg tcgcactgag ccgatttcat ctgcagctca
      181 ggacgacagc ttgtgaagca gccgtcatcc tgagcgccgg cgaaggatcc cttgcgcccg
      241 accttcgcgg caaaccagcc gcattccgca ttcaatacac ccgttccacc gaatacccgc
      301 gctgcctcag cagggccggc agcccgttct tgccgatcag gtgcagcagt ccgacggcga
      361 cgaagctgct ttctcgctcc ttcagcagaa tttcgacctt gtccgccatc agcggattgc
      421 gctcgtcgag cagtacccgg tgcgtgaact cggcggtgcg gctgccttcg gtcagcgact
      481 cctgccaaag cctggcgagc gtgtcgcggt ccgcactcgc ccaggcatcg atcagctcgc
      541 gtgccttgcg cagcgcgcgt cccgcctgca gatcctgcag cgtctcgcgc aggtattcct
      601 cctgcgcggc cgcgctcatg ccgtcgaaca aggccatctg gtactcggcc gtctcaagtt
      661 ccagcaccgc gtggcccggc gcaaaggcga tcaggttgta ctccgcgccg tagtggcggc
      721 gaaagccgtt gcggtccagt tccaggccct gcagcagatt ggcgaccagc catggtttca
      781 ttccttgcac cgcttcgtag gcgaggccga agcgctccag cgcgtctttc agcagccgca
      841 gggtgtcggc gtcgagatgc cggtctatgc ccgtccccgg cgaatagagt ccgtgcttct
      901 cgagcgcgcg atgtaccggc gcgtcgtcac gcacgtcaag ttcgagcacc agccggcggc
      961 tctgcatcag cgcccgctcg acttccgcat caagaaccat gtgagccggc gtgccgacgt
     1021 ggatggtgcc gaacaggtag ctgaccgtgc cttcgtggcg cacgcggtac agcgccccgc
     1081 gcgtaggtgc cgcgacgctc gcatcccggc cggcttgtat agtgacagcc gctgctgcat
     1141 cattgcccgc gtgcgccggc gccagcagcg cggccgacaa cagcgccgtc atccataatt
     1201 tcatgttttc ctccccgccc gtccatctcg ggccgccgag aatctagcag atgacccgac
     1261 cgatttggtt gttcgacctt gacaacacgc tgcacaatgc ctcgcaggct gtgttcccgg
     1321 cgattaacgc caacatgaat gcctacatgg cgcgcgtgct tggcagcgac ggcacgccgg
     1381 ccgcgccgga cgtggtcaat gcgatgcggc tggcgtactg gaaacgctac ggcgcgacgc
     1441 tgctgggcat ggtcaagtat caccaggtga agatggagga gttcctgatc gaggcccatc
     1501 gcttcgatga cctgccggcg atgctgcgcg ccgagcgcgg gctggcgcgc ctgctcaagc
     1561 ggctgcccgg ccgcaaggtc ctgctcacca atgcgccacg ccggtattcg cgcgaggtgc
     1621 tgcaccactt cgggctgcat cgccacttcg cgcgccacat tccgatcgag gccatgcacg
     1681 tgcaccgcca gctgcggccc aagccgtcgc gccatctgct gcgcaagctg atgcggcgcg
     1741 aaggcgccgc cgcgcaccgc tgcatcctgg tcgaagacac gctcgacaac ctgaaggcgg
     1801 cgaagatgct tggcatgcgc acggtgtggg taacccagta cctcgcgtcc aatccccatt
     1861 tgcgcccggt tccggcggat cgtccgctgc cggcgttttc caagcgcccc gtttatgtgg
     1921 atgtcaaagt aaaatcggtc acacaattgc ccgcggcgct gcatcggctg cgcatacgaa
     1981 ccagataaca ccatggcaac gacaaaaccc ggggagcgca agctgcagat tctccagatg
     2041 ctcgcgcaga tgctggagca gccgaaagga gagaaggtca cgaccgcggc gctggccgca
     2101 cggctggacg tctccgaggc ggcgctgtat cgccacttcg cgagcaaggc ccagatgttc
     2161 gagggcctga tcgaattcat cgagtccacc gtgttcggac tcatcaacca gatcaacgag
     2221 cagcaggaaa gcggcctcgc gcaggcccag gcgatggccg gcatgctgct cggtttcgcc
     2281 gagcgcaatc ccggcatgac gcgcgtgctg atcggcgatg cgctggttaa cgaggatgag
     2341 cggctgcagg cgcgtatgaa ccagttcgtc gaacgcgtcg agatggcctt tcgccagtca
     2401 ctgcgcctgg ccgagaccca gggcgacgcc gacgaagtcg acgccggcgc gcgcgcgtcg
     2461 atgatcacca gcttcgtggt cggccgctgg caccgctacg ccaagagcgg gttccggcag
     2521 aagccgaccg agcaggcgca gatcggcttt ctgatgcgct gagtgcaagt ccttttgcga
     2581 cgccctcgaa tacagtgcgt tgccccccgc tttgtcatcc tgagccgcag gcgaaggatc
     2641 tcatccgctt gatcaagcgg cgtacggcat ggcaagggat gaggtccttc gctggcgctc
     2701 aggacgacag gaggtcggtg tcatcgcccc caccctgtca tcctgagccg caggcgaagg
     2761 atctcatccg ctcgatcaag cggcgcccgc tatggcaatg gatgaggtcc taggctgcgt
     2821 tcaggacgac aaggctgccg cagtcttgct ggcgcgtctg ccaacccgcc aattccccgt
     2881 tacactccgt tctttttgcg ctgccgcgct ccatgtcctc cgccaaacct ttccccgttt
     2941 ttgcctttgc cacgctcctg atcggcggca tggccattgg cttcgccggc atcttcatgc
     3001 gcctgtccga tgtcaatccg ctcgcgtcgg ccttctggcg catggcgctg gcggcgccaa
     3061 tcctgtgggc ttgggcattt gcggtgcgca ggcaggatgc ggcggccggc aagcgcacgg
     3121 acttcagcaa ggcgctggtg ctggccggcg tgcacttcgc cgccgacatg gcgctgtggc
     3181 acctgtcgct gcattacacg acggtcgcca atgccacgct gctgtcgaac ttcgcgccta
     3241 tcttcatcgc gctctggctc tggctggcct accgggtcaa gttcgcccgc atcttcatca
     3301 tcggcatggt gctggctctt gtcggcgcgg cgctgctggt ggggccgaat gcggcgggcg
     3361 ggccggacaa gctgctcggc gacgcgctcg gcctgtccag cgcaatcttc tatgccgctt
     3421 accaattggt gatcaaggac gcgcgcaacg actactcgac cgcgcgcctg atggcctgga
     3481 gcacgacggt cacagggctt gcgctgctgc cgttcgcgct gctcgcgccc ggtccgttct
     3541 tcccggccgc gctgatcgga tggctgccgc tgctgggcct cgcgctggtc gcccagatcg
     3601 gcgggcagac ggtgatcgcc tacgcattcg cgcatctgcc cgcctcgcta tcctcggtca
     3661 gcctgctgat ccagccgctg accgcggcgg tggccgcctg gctgattttc caggaggcga
     3721 tcctgccggt gcagatgctg ggcggcgcgc tgctgctgtg ggggatttac ctcgcgaaga
     3781 aggggagctg agccgggctt gagccaggct tacaatacgg ggctggcctc gccgccctcg
     3841 ttctcgccat cccgccaacc caccgtgccg cacgccattt cgcccgacgc ctgctttgcc
     3901 ttgctggacg acagcagcgc agaaacgcgc cgctcgcgcc tgtacacagg cctggtgcgc
     3961 acgctcgcct gctacaccgc tcggtcattg ccggccctgc tggacgacat gcagcatgag
     4021 ctcgccgccg gccgttttgc ggtcggattg ttcgagtacg aactcggcgc gcgcatgcac
     4081 ggggtactgc ccgacgatgc ggccgcgtcg ccgctcgcac agatcctgct gttttcctca
     4141 tgccagttgc tggatgcaac agaaaccgac caatggctgc gcgcgcatga tgatggcgcg
     4201 ccggccggcg tggccggcat ccgtgcatcg gtcgacgcgc aagcgttcag cgacgcgatt
     4261 tcgcgcatcc atgcctggat cgaggccggc gacacctacc agatcaacta cacgttcagg
     4321 ctgcatttcg atgcctatgg cacgccgctc gcgctctatc gcaggctgcg cgcgcgccag
     4381 ccggttccct atggcgcgct gatcgcgctg ccggacggca aagcggtact gtcgctgtcg
     4441 cccgaactgt tcgtgcgcca tgacggcggc gtgctcaccg cccgcccgat gaagggcacc
     4501 gcccccgcct gcggcgacgc aaacgaggac gcaaggcggg cggccgcact ggcggccgat
     4561 ccgaagaacc gcgccgagaa cctgatgatc gtcgacctgt tgcgcaacga tcttgggcgc
     4621 gtggccgagt tcggttcggt gcgcgtgccg cagctgttcc aggtcgaacg ctttgccagc
     4681 gtgctgcaga tgacctcgac cgtggaagcg cggctgcagc agggcacgac gctcgcgcag
     4741 atcttcgccg cgctgtatcc gtgcgggtcg atcaccggcg cgcccaagcg caggacgatg
     4801 cagatcatca acgcgctgga gtccgagccg cgcgggatct acacgggcgc gatcggctgg
     4861 ttcgaagcgc cggaagatgg tctggcgacc ggcgacttct gcctgtcggt gccgatccgc
     4921 acgctcgtcc tcgacgcgcc ggcgcagggc gtacggcgcg gccggctcgg cgtcggcgcc
     4981 ggcatcgtgc atgacagcgt cgcggccgac gagtacgaag agtgccggct caaggcccgc
     5041 ttcctgaccg gattgcccgc aagcttcgca ctgatcgaga ccatccttgt gcaggcacgg
     5101 cgctgccgcc acctcgatct gcatctcgcg cggctgcgcg agtcggccgc gtacttcggc
     5161 tttgctttcg acgcgctgcg catacgcgac gcaatcgatg cagcctgtgc ggaactgccc
     5221 gcagatggcg ccttccgggc gcggctctgc gtggagcatg acggcgcgtg cgaagtgcaa
     5281 accggggcgc ttgcgccgct ggatgttccg gtcaaactgg tgctggcgaa cgagaaggtc
     5341 gattcgtccg acttgttcct gcggcacaag acgacgcagc gcagccgcta cgacgcggca
     5401 tggcgggaag ccgagcgcca gggagcgttc gacatgctgt tcttcaacga gaggggcgag
     5461 ctaaccgagg gcgcacgcag caatgtgttc gtgcgcctgg gcggccgctg gtacacgccg
     5521 ccgctgtcgg ccggcgtgct gcccggcgtc atgcgcagcg tgctgctggc agatccggcg
     5581 tggaatgcga gcgaacgcac gctcactcgc gccgatctcg gtcaggcaga ggaaatcatc
     5641 gtctgcaacg cgctgcgcgg cgccctaccg ggtacgctcg tctagcacgg cgaccgcctc
     5701 gctgcgcggc gcggtgagca ccagcgccat cgccagcagc acgaccatcg cgccgaccag
     5761 ggtcgacggg ccgacccgct cgtccaggaa aagcgcaccc cacagcatgc tgaacagcgg
     5821 aatcaggaag gacgccgaca ttgcgcgtgt cggcccgact ttcacgatca gcggaatgaa
     5881 cagcgcctgc gccagtcccg acgagagcac cgccatgccg agcacgcagg cgaacgccag
     5941 cgccgaaggc aattcggctg gcacggcgag tggagcggca ggcgcaagaa tgatgccgcc
     6001 agcaatcagc gaggccgccg ccatcgcgat cggatggatg ccgcccgggc gtccggtctt
     6061 cttcaccacg atgctcgata ccgcataggc gctcgagccg acgaggcagg ccgcactcgc
     6121 aagcagcgtc gagacgccta gttccaacgc gcccgcgccg accagaatcg caacaccgac
     6181 caccccgagc atgagcccgg ccagcttgcg cagcgtgagc cgttccgaca gccagatcac
     6241 cgacagcagc gcaccgaaca gcggcgccgt ggagttgagt accgccatgt gcgcggccgg
     6301 cagatgcagc gcggcgaacg agaatgcgca gaacggaatc gccaccgcgc acagtcccac
     6361 cgctgcatag ggcttgacgt tctcgcgcca gtgcaggcgc acgccggttg cgcgggcgaa
     6421 tccgtacagc acggccgcgg ccagcgcggt gcgcaggaag gccgtcagca ccggtccgaa
     6481 ctcgggaacg gcgacgcgca ggaagatgaa ggatccaccc cagattgcgg aaagaaggac
     6541 gagcttgacg acgtcggcgg cggacatgag ctggatggtg gcgaagggcc ggtcgattct
     6601 agcagcaggt ttgtcgccgc gcagaccggc ttgcgatcgc gggtcctgcc gcgtcatccg
     6661 gttgatacaa cataaccggt ccattccctg cgcgtcgaat aatcgcagca ggagaaaaca
     6721 agatgaagat caaagggaga ggcgcgccgt gcgccgcggc atcctggctg ctgcttgcga
     6781 gcgccctggg tggttgcgcg cagtatccgc atcgcgcgca gcagcacgtc gatgctgctg
     6841 gccagcgcca tttcgaagcc tacctgcagt accgcgccag agcaggccgg gaaggaatcg
     6901 tcgcgtccgg cagctccgcg ctcgacgcgt caggacacgc cggcgattcg cgcacgccta
     6961 tgcaattcag cgcgcattct tcaccgtgac gcggagcaga ccgggcagcc gtcgtttcgc
     7021 atcaccctga tgccggtcca ctccatgctg cgcgcatcga gcagcagcag gcgaccggcc
     7081 agcgactccc cgacacctgc gacgatcttc agcgcctcgg ccgcctgcgt cgcgccgatg
     7141 atgccgacca gaggcgcgaa cactcccatg gtcgagcact gcacttcctc gaaagcctga
     7201 tccggcggga acaggcaggc gtagcagggc gcgtcgggct ggcgcacatc gaatacggag
     7261 acctgtgcat cgaagcggat cgcggcgccc gacaccagcg gcacgcggtg cgatacgcag
     7321 gcgcggttga cggcgtgccg cgtgtcgaag ttgtcgctgc agtcgagcac cacgctggcg
     7381 ccggcaacga gttctccgag ccgcttcgcg tccgcccgct cgatcagcgc ctccacctcc
     7441 acttccggat tgatctgcgc gagtgtctgc ttgcccgact gcgctttcgg ctggccgacg
     7501 cgctcggtcg tgtgcaggat ctgacgctgc aggttggtca ggtccacgct gtcatggtcg
     7561 accagcgtga tcttgccgat gccggccgat gcgaggtagt aggcggcagg cgagccgagc
     7621 ccgcctgccc cgatcacgag cgcatgggcg gccagcaggc gctcctggcc ttcgatgccg
     7681 atctcgtcga gcaggatgtg gcgggaatag cggagcagtt ggttgtcgtt cattgacgca
     7741 gggaatgaag cgggaggtgg ctagcttacc acccgggcac tgatcttttc gccgcgagac
     7801 cggtcgatag ggcaggaggt gtgacatggt gcagcgtgat tcaccgcgcc atctgcgcgc
     7861 gctgttgctc gggccggggc tggtgatctt cctgtgggca ttgtgggcgt tcatcgcgtt
     7921 cgtgctgttt gaccgcgatg cccacgggct ggtgatcgtg ggggtcaccc tggtcctgtt
     7981 tttcatcgtc gtgctgttgc tgcgccggcg cggttgggcg gactggcgca cgggcttgta
     8041 cgccggactg ttcatcggcc tgctggtgct gatgtacatg atcccgatcc gtggcgcctt
     8101 gccgcccgac gcgcatgcgc tgaaccagcg cgccaacgcc gcgcacgcgg atcgctatga
     8161 ctacgcggcc tcgatcttcg acgaggtggt cgcgcgctac gttgcgccga cgcgcgaata
     8221 catcctccag ccgcaccgca tcttcctcgt caaggacacc gcctatttct ggaacgccca
     8281 aggcggctac gtaccctcgc acatccaggc gcagctctac cgcaagctgc tgctggccag
     8341 cgggcggttc gctgagcagg aagtcgagct gagaaccggc ggctgtgtga actcgccgca
     8401 cggctatgtg gtgatccatc accccgagcg ggaaatcttc gccgacctgt gggcggccga
     8461 ccagttcgag gagtaccggt tcgggcagcg ggtgcaaatg cccggctgcg acggagtcgt
     8521 cgccgagccc gaaggcgagc cggcgtgagc gcgatcaccg cattggttca tggcaaatgg
     8581 agtgtgataa tataacatca catttcatcc tgttaatgga gatgccgtgc cagccaacaa
     8641 gaaaacgacc cgcctgcttg cccccgtact gctctgcgcg gccattgccc ttgcagcgtg
     8701 cggcggcggc ggtcacgaag atgggcacca cgacgacgcc taccacatcg acaccgcagg
     8761 ccgcctcgtg attgcggccg acgccgccgc cagcgtgcgc gtctatgacc tggacagcag
     8821 ccaggtgcac gccacccaca cgctcgccaa tccggcctcc gccctctaca caagtcccgg
     8881 cggccgctat gcggtggtca tgcagcagaa ccaggatgcg gtccagttcg ttgatggcgg
     8941 catctggcag gaagatcatg gcgaccacat gcatgactac aaggcggcct cgaacatgat
     9001 ggcgtggacc cttgccggcc cgcgtccctc gcatttcgac gtgcagcatg gcaagcaggc
     9061 ggcgatcttc atggacggcc gcggcgacgc cgtgccaccg caatattcga cggtacgctt
     9121 gatcaccgac gcgtcgatcg ccggcggcag cgtgacggcc ggcctggacc tcgactacgc
     9181 ggtccatggc ctcgccgaac cgcgcgacga cgtgctgctc gtcgcgcacc gcgccacgga
     9241 tgcgccggat gcgctgccga cccacctgaa cgtgtac
//
LOCUS       DATLGF010000513         2063 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_124115, whole genome shotgun sequence.
ACCESSION   DATLGF010000513 DATLGF010000000
VERSION     DATLGF010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124115"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VM406_16020"
     CDS             complement(<1..269)
                     /locus_tag="VM406_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HVL77520.1"
                     /translation="MLEEVFTRFGMQEIHSDTIERQLAEAGDRLVCIFCWGEDCFNCE
                     VAKKAMLAKPEAIEKLELVWMHGNVYADRALGLKFGLHGVPVFVY"
     gene            complement(333..860)
                     /locus_tag="VM406_16025"
     CDS             complement(333..860)
                     /locus_tag="VM406_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006760961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HVL77521.1"
                     /translation="MEFLNAIKSQIPDYAKDIRLNLDSVVARSSLDPADAVGAMLASA
                     FSLRSKTLMDAILNSGQLSETDANAALTAAALMGMNNVWYPYVEMAEDNDLKTQRAEL
                     RMNAYATHGGIDRKRFEIFALASSIIGRCHFCIQSHYKLLKDSGLTATQLRDIGRIAA
                     VATAAAQVLSAQGAT"
     gene            complement(928..1476)
                     /locus_tag="VM406_16030"
     CDS             complement(928..1476)
                     /locus_tag="VM406_16030"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HVL77522.1"
                     /translation="MKTVGDKLESFSVTGVKPGFNHHEENGQSAFEPITEKSFPGKWK
                     VIYFYPKDFTFVCPTEIVGFAQLAKDFEDRDAVLLAGSTDNEFAKLGWRREHKDLNGL
                     NHWQFADTTGSLVDQLGVREKSAGVALRATFIVDPDNTIQHVSVNNLNVGRSPQEVLR
                     LLDGLQTDELCPCNREVGGKTL"
     gene            complement(1660..>2063)
                     /locus_tag="VM406_16035"
     CDS             complement(1660..>2063)
                     /locus_tag="VM406_16035"
                     /inference="COORDINATES: protein motif:HMM:NF017833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HVL77523.1"
                     /translation="FMGVMVLLITSQLLMGRDSFWLPNWLVRRSVSRSKLDKALHLLR
                     PPARWIDRGVRPRLCALTGPRGAWCIALTCTLLGLSMPPMEIVPFSVNVVGLILATFG
                     LSLIGRDGLLALAAFILTGLLAAGLTWYALG"
BASE COUNT          389 a          620 c          658 g          396 t
ORIGIN      
        1 tagtacacga acactggcac cccgtgcagg ccgaacttga ggccgagcgc gcggtcggcg
       61 tagacattcc cgtgcatcca gaccagttcg agcttttcga ttgcttccgg cttggcgagc
      121 atggccttct tcgccacctc gcagttgaag caatcctcgc cccagcagaa gatgcatacg
      181 aggcgatcgc cggcttcggc aagctggcgt tcgatcgtat cgctgtgtat ttcctgcatg
      241 ccgaaacggg taaaaacttc ttcgagcatc gtatcgactc caaaaaaaaa cgcggcccgc
      301 ggccgcgttc atacagggac gccgggcttc gctcaggtcg cgccttgcgc ggacagcacc
      361 tgcgccgcag ccgtcgccac cgcggcaatg cggccgatgt cgcgcagctg ggtggcggtc
      421 agcccgctat ccttcagcag cttgtagtgc gactggatgc agaagtggca gcggccgatg
      481 atcgacgagg ccagcgcaaa aatctcgaaa cgcttgcggt cgatgccgcc gtgcgtcgca
      541 tacgcgttca tccgcagttc cgcgcgctgg gtcttcaggt cgttgtcttc agccatttcg
      601 acatacggat accagacatt gttcatgccc atcagcgcgg ccgcggtcag cgcggcattg
      661 gcgtcggttt ccgacagttg gcccgaattc aggatggcgt ccatcagcgt cttggagcgc
      721 agcgagaacg ccgaggccag cattgcgccg acggcgtccg ccggatccag gctggagcgc
      781 gccaccactg aatcgaggtt cagccggatg tccttggcgt agtcggggat ctgcgacttg
      841 atcgcattca ggaattccat acgtgctcct tgagatcagg gagagagcgt tgccgcgccg
      901 gcggcgcggc aacgcagggg aggggactta cagggtcttg ccgccgactt cgcggttgca
      961 cgggcacagt tcgtcggtct gcaggccgtc cagcaggcgc agcacttcct gcgggctgcg
     1021 gcccacgttc aggttgttca cagacacgtg ctggatcgtg ttgtccggat cgacgatgaa
     1081 ggtcgcgcgc agggccacgc cggcgctctt ctcgcgcacg ccgagctggt cgacgaggga
     1141 gccggtggtg tcggcgaact gccagtggtt cagaccgttc aggtccttgt gctcacggcg
     1201 ccagccgagc ttggcgaatt cgttgtcggt cgaaccggcc agcagcacgg cgtcgcggtc
     1261 ttcgaagtcc ttggcgagct gggcgaaacc gacgatttcg gtcgggcaga cgaaggtgaa
     1321 gtccttcgga tagaaataga tgaccttcca cttgccgggg aaagattttt cggtgatggg
     1381 ttcaaacgcc gattggccgt tttcttcgtg gtggttgaag ccgggcttga cgccggtaac
     1441 ggagaacgat tcgagcttat cacctacggt tttcatgtat tcactcctgt tgtgatgtgg
     1501 ttgcgggtaa tgacaggaca gagcataacg aacgcggttc tatttgtaaa agtgattatc
     1561 tttatgatcg acatttacaa aagctatgct gcagcccgtt ctcgcaagcg ccgatgatgc
     1621 gtgtcacgct gttcacgatg gttgcgctgg atcaatcttt cagccgagcg cgtaccaggt
     1681 caggccggcc gcgagcagac cggtcaggat gaaggccgcg agcgcgagca ggccgtcgcg
     1741 gccgatcagc gagagcccga aggtcgccag gatcaggccc accacattga cgctgaacgg
     1801 cacgatttcc atcggcggca tcgacagccc gagcagcgtg caggtcagtg cgatgcacca
     1861 cgccccccgt ggcccggtca gcgcacacag gcgagggcgc acgccgcggt cgatccagcg
     1921 cgcaggcggt cgcagcaagt gcagcgcctt gtccagcttg ctgcgcgaca ccgagcggcg
     1981 caccagccag ttcggcagcc agaaactgtc gcgtcccatc agcagctgcg aggtaatcag
     2041 cagcaccatc acgcccatga agg
//
LOCUS       DATLGF010000514        10542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_220512, whole genome shotgun sequence.
ACCESSION   DATLGF010000514 DATLGF010000000
VERSION     DATLGF010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 10542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10542
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220512"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /locus_tag="VM406_16040"
     CDS             complement(<1..1379)
                     /locus_tag="VM406_16040"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HVL77524.1"
                     /translation="MKNLFRFPALLAGAYGLGRSLILALIVSLLAFFGAFQSADLRLY
                     DFFVRHTPELSPVQRQVVLLEGHAPFFERGGVDWLRLVDELVSRGAEQVVFSVRPEGS
                     DAMLSALSAHSKVVLGAPVDQPSPQADREPGQAQSALPAAAIPGALNGMHRWQDTRHR
                     GNTESIPALEAVAARRLGAEVPDSGPYLIDFRHPASGFGRIALDEALAGQLASSLVKG
                     RVVLVGPAAERTHRTYATPREDARGEISALEYHAYALDALLRGTAIAMPPRFADVGLL
                     LVLGALCFLAFQPMTFRKAVAVASGVALATLGLAWSMLALHMHIPVVGALLMLGATLL
                     AVFQSKTERQNEALERMVMETHLASTQSGGNNQGARDAFWPHAAAMIDQILPLNRMVL
                     LERMGQGKHLREVYSLRCQIDTVAERRRDFERAPYRNAVDKDAAIEVSGYLKAGTDDE
                     QQFLMPLAWG"
     gene            complement(1597..2577)
                     /locus_tag="VM406_16045"
     CDS             complement(1597..2577)
                     /locus_tag="VM406_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77525.1"
                     /translation="MFLICRAARACFAGISPMGLAIHIAWRRTPHMQCRLPGFFNNTY
                     RSQAMKKNLLLTGALVLLAGCGGGEYDIGTVAVHGTVQSCDAASGANARVVLPYGEDQ
                     SFETTTDADGNFVLDVDADAIPRVSQVALGVYGESDACQPGELLVAGLAAAAKTKRVD
                     LPAKKLEDLKPAHFVMSSPGRPLIHLGDDQYRGPANSKLQVATHGVQIENLIGTFTQD
                     FKDRFNTAYIEFSARGMQEESMLCATHYENSIGLVATGGGLAEPVIRTVRPDASSPNG
                     NFTRYSIPIDLTGIPAGASVSLVASSGKCEAGGTDHDDFELSGLLVRFAH"
     gene            complement(2979..4439)
                     /gene="tldD"
                     /locus_tag="VM406_16050"
     CDS             complement(2979..4439)
                     /gene="tldD"
                     /locus_tag="VM406_16050"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HVL77526.1"
                     /translation="MNLFEPNLQTLGIAREILLAPFGLDETHLSRAMAAIFAHRVDYA
                     DLYFQFSKNEGWSLEEGIVKTGSFSIDQGVGVRAVSGDKTAFAYSDDISERALLDAAA
                     ATRTIARQGAGKVKVASSIRPAGGRSLYLPHDPLTSLESTAKVRLLERLERIARAKDP
                     RVVQVMASLAGEYDVVMVVRSDGVLAADVRPLVRVSITVIAEQNGRREMGSAGGGGRY
                     NYAYFTDEILEKYATDAVNSALVNLEARPAPAGQFTVVLGSGWPGVLLHEAVGHGLEG
                     DFNRKGSSTFAGRIGERVAARGVTVVDDGTLADRRGSLNIDDEGNPTQCTTLIEDGIL
                     KGYIQDTMNARLMKMPVTGNARRESFAHLPMPRMTNTYMLAGDREPEEIIASVKNGLY
                     AVNFGGGQVDITNGKFVFSASEAYMIEDGKISYPVKGATLIGNGPEVLNRVSMIGRDM
                     RLDSGIGVCGKEGQSVPVGVGQPTLRIDGMTVGGTA"
     gene            complement(4541..5347)
                     /locus_tag="VM406_16055"
     CDS             complement(4541..5347)
                     /locus_tag="VM406_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HVL77527.1"
                     /translation="MNAPTRIAAVQMVSTPEVEQNFETAQQLIGEAAQQGARLVLLPE
                     YWPVMGMQESDKVRHAEQFGDGPIQRFMAEIAERHRIWLIGGTLPMVSPEQDKVLNTT
                     MVYNERGEHVIRYDKIHLFSFTKGEESYDEARTIVHGSKVASFESPWGRVGLSVCYDL
                     RFPELYRAMGDCSLIVMPAAFTYTTGRAHWEVLLRARAIENQCYVLAAAQGGHHPNGR
                     RTWGHSMLVDPWGEIKDVRPEGAGIVIGDLDPAHLARVRESLPALRHRRL"
     gene            complement(5352..9563)
                     /locus_tag="VM406_16060"
     CDS             complement(5352..9563)
                     /locus_tag="VM406_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HVL77528.1"
                     /translation="MSDPVQAQKAAPSVFLRCWHILRGCWRILHHFFGRTFSFLLAAA
                     IVAYFAFCALVLGIRYVVLPNIDAYKPQIEALASEAIGNPVTIGALNASWSGLHPRLA
                     LDSVVIHGHDGKPALSLPSVAATLSWWTIAAGELRLNSLEIDRPDLEIARDAQGRLHV
                     AGIQLAAGQQSDGRGADWVLSQREIVIRGGRLRWIDNLRGAPELVLDKVDFVLHNRWR
                     RHRFALHAQPPDGLAAPLDIRARFEHPPFARRISDVSRWKGEMYVDLRDTDLAAWRAW
                     IDYPIDMESGHGSVRAWLDFERAKVADFTADLGLANVVARLQPDLEPLRLKEVSGRIA
                     LREEISPGNDDNTPTFGTNGHAVALTDFSLRTEDGLYLPRTTISESYTPAARGRPART
                     EIQARMVDLETLANFATRLPLPADQRRLLADLAPRGVVRDFSAQWHGVYPAVSAYNVR
                     GQFTGLSLKAQPARPAVAGAAGRPGQAAVPAIPGFANLSGRIEASERGGMLSLASEDV
                     VLDTPGYFAQAAMPFERLAAQANWSFQDRNQLVLDLQRFDFAQEGVSGSLSGKHVLNL
                     DAKGGESPGTIDLSGQIARLEVDRVRRYLPLQTPEHLHEWLSGALLGGHAEDVRIRVK
                     GDLAQFPFATLAARDKPKGEFSVSGRIVNGSLNYAPWGHSPDGKKPEWPIIDQINGRI
                     AFDRNRMDIFADSARSSGTQLSRVKVVIPDLLADEPLLTVDGEAAGPLQNFVRFTHDS
                     PVGQWIDNFTETTRATGSARLALQLQLPLAHMEDAKVRGTLQFAGNTVHLFEPLPPLS
                     GASGALEFNEKGFSLPGVRANFLGGPVSLNGGTQRDGSILVRADGAMSAAGVRQAYAM
                     PMTSRATDRITGGTRYLATVRVRDGNPSVMVESNLVGLGLDFPEPLRKPAQDSLPLRF
                     EMVQSPASDSLLLRDQIRLSLGTMIQAHYEREKPATPQAAWRVVSGGIGVNAPAPEPD
                     SGVYANVNVPSLDLDAWRRAMSSALGPAPQGDGARRSQALSIEQYVEPEVLAAQAREL
                     LIMDRKLNNVVVGASHQDGVWQANIDSDQVSGYLSWNEPRSGRGMGRVTARLTSLVIP
                     EAAKSGVADLLEAPGSTEQLPAVDIVAENFELFGKRFGRLTLQARNARGPEGREWRIN
                     KLALTNPDGELQATGKWSGRAAASRSTLDYSLSIVDAGRLLDRFGFPKLLRGGKGRME
                     GNISWDGPPVSLDVESLSGRIKLDVASGQFLKVDPAAQGAAKLLGVLSLQSLPRRLAL
                     DFRDVFSEGFAFDGVNAVATISNGVARTENFKMRGVSATVLIDGTADIAKEDQNLHVV
                     VIPEINAGAASVVYGLAVNPAIGVGTFLAQLFLREPLMRAFTFEYEVKGPWKEPVVTR
                     LARRNGADAAQDTNGELAIKLEANG"
     gene            9863..>10542
                     /locus_tag="VM406_16065"
     CDS             9863..>10542
                     /locus_tag="VM406_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamine synthetase
                     adenylyltransferase/deadenyltransferase"
                     /protein_id="HVL77529.1"
                     /translation="MPLHRAMRRVRNLVVSALIERDLSGRADLDEVLEAISAFADFAV
                     QRHVAALTEEMRAAHGTPVGAESGEPQELIVLGMGKLGGRELNVSSDIDLIFVYPEDG
                     ETQTTAPGQRPLSNHEFFARLGKKLIAALSEITEDGFTFRVDMALRPNGASGPLAASF
                     GMVEEYLIVQGREWERYAWVKARALTGRPQDIAALEQIVQPFVYRRYLDFGAIEAMRN
                     MHAQIRAEV"
BASE COUNT         1869 a         3469 c         3397 g         1807 t
ORIGIN      
        1 ccccaggcca gcggcatcag gaattgctgc tcatcgtccg tgcccgcctt cagatagccg
       61 gagacctcga tcgccgcatc cttgtccacg gcattgcggt acggtgcccg ttcgaaatcg
      121 cgccggcgtt ccgcgaccgt atcgatctgg cagcgcaggg aatagacttc gcgcagatgt
      181 tttccctgcc ccatgcgctc gagcagcacc atgcgattga gcgggaggat ctggtcaatc
      241 atcgcggcag cgtgcggcca gaacgcgtcg cgcgcgccct ggttgtttcc gccggactgc
      301 gtgctcgcaa gatgcgtctc catcaccatc cgctccagcg cctcgttctg gcgctcggtc
      361 ttgctttgaa agacggcaag cagcgtcgcg cccagcatca ggagcgcgcc caccacaggt
      421 atgtgcatat gcagcgccag catcgaccag gccaggccga gcgtggccag ggcgacgccg
      481 gaagccaccg cgacggcctt cctgaaagtc atgggctgga acgcgaggaa gcacagggcg
      541 cctaatacca gcagcaggcc cacgtccgcg aaacggggag gcatggcaat cgcggtaccg
      601 cgcaacaggg catcgagcgc ataggcgtga tactccagcg ccgagatttc accgcgcgca
      661 tcttcacgtg gagttgcata ggtccggtgc gtacgttcgg ccgcaggccc caccagcacg
      721 acgcgccctt tcaccaggga tgacgccagc tggcccgcca gcgcctcgtc caatgcgatg
      781 cgtccgaagc cgcttgccgg atggcggaaa tcgatcaggt aaggcccgct gtccggaacc
      841 tcggcgccga ggcgccgcgc tgccaccgct tccagggcgg ggatggattc ggtgttgccg
      901 cgatgccgtg tgtcctgcca gcgatgcatg ccattgagcg cgccgggaat cgcagccgca
      961 ggcagggcag actgggcttg cccgggctca cgatcagcct gaggcgacgg ctgatccacg
     1021 ggcgcaccga gtacgacttt cgaatgcgcg ctgagcgcac tcagcatggc atcgcttccc
     1081 tccggccgaa cgctgaacac gacctgttcg gcaccgcggg aaaccagttc gtcgaccagg
     1141 cgcaaccagt cgaccccacc cctctcgaag aacggtgcat gcccctccag cagcaccact
     1201 tgccgctgca cgggtgacag ctcgggagta tggcgaacga agaaatcgta gaggcgcaga
     1261 tcggcggact ggaatgcgcc gaagaacgca agcaatgaaa cgatgagagc aaggatcagg
     1321 cttcgcccaa ggccgtatgc gccggccagc aaggcgggaa atcgaaacaa gttcttcatc
     1381 tgcgtgggga ggtcaggtta ggctgtgacg cgcgtgcgca gtcaaggccg agacggttgg
     1441 cgctgccggt gacagaacgc tggcagctgc attgctagtt gttctatgcg aattgccgaa
     1501 ggccgttgta gcgtccggca aaaattgaat cggacgcttg ggggaacgac gtcgggccga
     1561 gctcacccgg acatcgttcc ctgcccttgc tgcgccttaa tgcgcgaaac gaacgagcaa
     1621 gccgctcaac tcgaaatcat cgtggtcggt gcctccggcc tcgcacttgc cggaagatgc
     1681 gaccaggctg acgctggcgc ctgccgggat gccggtgaga tcgatgggaa tgctgtaacg
     1741 ggtgaagttg ccgttcgggc tgctggcatc cggacgcacg gtgcgaatga caggctcggc
     1801 aagtccgccg ccggtggcga ccaggccgat actgttctca tagtgcgtcg cgcacagcat
     1861 cgactcttcc tgcatgccgc gcgcggaaaa ctcgatataa gccgtgttga agcgatcctt
     1921 gaaatcttgc gtgaaggtgc cgatcaggtt ctcgatctgc acgccgtgcg tggccacctg
     1981 gagcttggag ttggcggggc cgcgatactg gtcgtcgccg aggtggatca agggacgacc
     2041 gggcgaggac atgacgaaat gagcgggctt cagatcctcc agcttcttgg cgggcaggtc
     2101 gacgcgcttc gttttggcag ccgcggccag gccggctaca aggagctcac ccggctggca
     2161 ggcgtcggac tcgccgtaga cgccgagcgc aacctgagac acgcgcggga tcgcatccgc
     2221 atccacatca agaacgaagt tgccatcggc atccgtggtg gtttcaaagg attgatcctc
     2281 cccgtatgga agcaccacgc gcgcgttcgc gccactggcg gcgtcgcagg attgcacagt
     2341 gccgtgaacg gctacggttc cgatgtcgta ctcgccacct ccacatccgg ccagcagtac
     2401 caacgcccct gtcaacagta aatttttttt catcgcttga gatcggtaag tattgttaaa
     2461 gaagcccggc aatcgacatt gcatgtgcgg tgtccgccgc catgcaatgt gtattgccag
     2521 ccccatcggg cttatccctg cgaagcatgc tcgtgccgcg cggcaaatca ggaacaagag
     2581 ggcgtattgt tttcgctcct ttcctaattg ctatttgctg ttgcgaaaaa ttacctggct
     2641 aacattgcgc tgaccgttac tacaagaact tccgccgccc gcgatggtcg gcgatgcgaa
     2701 gtcgcgtgtt tcagtgagct cgtcgcgcaa gtcgtgcgca gtgtgcgcag catgcgccgg
     2761 acgatggagg cgggagacgc gaatgccgtt cgggcccggg gtgtttgaag cgccagaacg
     2821 aattgaacga acgggcgaca ccgtgcaatg ccgccctgaa tcaagaaagg atgggtcgct
     2881 aatggcgagc gttccacgcg gcaggcaccg cgtggttgcg ccggcctagt tgattactgg
     2941 ccgggaggag aggagctgtc gttgtgttga ggccctgctc aggcggttcc gccaaccgtc
     3001 atgccgtcta tgcgcagcgt cggctgcccg acgccgaccg gcacgctctg cccttccttg
     3061 ccgcacacgc ctatgccgct gtccagccgc atgtcgcggc cgatcatcga aacccggttc
     3121 agcacctcgg gaccgttgcc gataagggtc gcacccttga ccgggtagct gatcttgcca
     3181 tcctcgatca tgtaggcctc gctggcggaa aacacgaact tgccgttggt gatgtcgacc
     3241 tgcccgccgc cgaagttgac cgcatacagg ccgttcttca ccgaggcaat gatctcttcc
     3301 ggttccctgt cgccggcgag catgtaggta ttcgtcatgc gcggcatagg caggtgggca
     3361 aacgattcgc ggcgcgcatt tccggtcacc ggcatcttca tcaggcgcgc gttcatcgtg
     3421 tcctggatat agcccttcag gatgccgtcc tcgatcaacg tcgtgcactg ggtcgggttg
     3481 ccctcgtcgt cgatgttgag cgacccgcgt cggtcggcca gcgtgccatc gtcaaccacg
     3541 gtgacgcccc ttgcagccac gcgctcgccg atgcggccgg cgaacgtgct ggaacccttg
     3601 cggttgaaat cgccctccag accgtgaccg accgcttcat gcagcagcac gcccggccag
     3661 cctgacccga gcacgacggt gaactgtccg gccggcgccg ggcgcgcctc caggttcacc
     3721 agcgccgaat tgacggcgtc ggtcgcatat ttctccagta tctcgtcggt gaaatacgca
     3781 tagttgtagc gcccgccgcc gccggccgaa cccatttcgc gccgaccgtt ctgctcggca
     3841 atcacggtga tcgatacgcg caccagcgga cgcacgtcgg cagccagcac gccatcgctg
     3901 cgcaccacca tcacgacgtc gtactcgccg gcaaggctgg ccatcacctg gaccacgcgg
     3961 ggatccttcg cacgcgcgat gcgttccagc cgctccagca gccgcacctt ggccgtggac
     4021 tcgagcgagg tcaacggatc gtgcggcagg tacagtgaac gtccgccggc ggggcggatc
     4081 gaggatgcga ccttgacctt gcccgcccct tgccgcgcga tcgtgcgcgt ggccgccgcc
     4141 gcatccagca gcgcgcgctc ggagatgtca tcggagtagg caaacgcggt cttgtcgccc
     4201 gacacggcgc gcacgccgac accctggtcg atcgagaagc tgccggtctt gacgatgcct
     4261 tcctccagac tccagccctc gttcttgctg aactgaaagt agaggtcggc gtagtcgacg
     4321 cggtgcgcga agatcgcagc catggcccgg ctcagatgcg tttcatccag gccgaatggc
     4381 gcaagcagga tttcgcgggc aatgcccagg gtttgcagat tcggttcgaa caaattcatg
     4441 gcgcgctcgg atgcggcggc gcatggccgc ccgatggagg gatgatggga tgaagggatt
     4501 ctagaacatc gaccgccgca gcgccggccg gcggcgcgcg tcagagccgg cgatgccgca
     4561 gcgcggggag gctttcgcgc acccgcgcca gatgggccgg gtcgaggtcg ccgatgacga
     4621 tgccggcgcc ttccggccgc acatccttga tctcgcccca gggatcgacc agcatgctgt
     4681 gtccccaggt gcggcgacca ttcggatgat gcccaccctg cgcggcggcc agcacgtagc
     4741 actggttctc gatcgcgcgt gcgcgcagca gcacttccca gtgcgcgcgg ccggtggtgt
     4801 aggtgaacgc ggccggcatc acgatcaggc tgcagtcgcc catcgctcga tagagctccg
     4861 gaaagcgcag gtcgtagcag acggacagcc ccacccttcc ccagggcgat tcaaagctcg
     4921 ccaccttgct cccatgcacg atggtgcgcg cctcgtcgta ggactcctcg cccttggtga
     4981 aactgaacag gtggatcttg tcgtagcgga tcacgtgttc gccgcgctcg ttgtagacca
     5041 tcgtcgtgtt gagcaccttg tcctgctcgg gcgacaccat cggcagcgtg ccgccgatca
     5101 gccagatgcg atggcgctcg gcgatctcgg ccatgaagcg ctggatcgga ccgtcgccga
     5161 actgctcggc atggcgcacc ttgtcggatt cctgcatgcc catcacgggc cagtattcgg
     5221 gcagcaggac cagccgggcg ccctgctgcg ccgcctcgcc gatcagttgc tgcgcggttt
     5281 cgaaattctg ttccacctcg ggggtggaaa ccatctgaac tgctgcaatt cgtgtcggtg
     5341 cattcatcgc gctatccgtt cgcttccagt ttgatcgcca gttccccgtt cgtatcctgc
     5401 gcagcgtccg cgccgttgcg gcgtgcaagc cgggtcacca ccggttcctt ccacggccct
     5461 ttcacctcgt actcgaacgt gaaggcccgc atcagcggct cgcgcaggaa cagctgcgca
     5521 aggaaggtgc cgaccccgat cgccggattg accgcaaggc cgtaaacgac cgaggccgca
     5581 ccggcgttga tctcggggat caccaccaca tgcagattct ggtcttcttt tgcgatgtct
     5641 gccgtgccat cgatcagaac ggttgccgat acaccgcgca tcttgaagtt ctcggtccgt
     5701 gccaccccgt tcgaaatggt ggcgacggcg ttgacgccgt cgaacgcgaa accctcggaa
     5761 aacacgtcgc gaaaatcgag cgcgagccgg cgcggcagcg actgcaggct gagcacgccg
     5821 agcagcttcg ccgcaccctg agcagcaggg tcgaccttga ggaactggcc gcttgcaaca
     5881 tcgagcttga tgcgcccgga caagctctcc acgtcgagcg acaccggcgg cccgtcccaa
     5941 ctgatgttgc cctccattct gccttttccg ccgcgcagca gcttcggaaa accgaagcgg
     6001 tcgagcaggc ggccggcatc aacaatggaa agcgaataat cgagggtgga gcggcttgcc
     6061 gccgcccgtc cggaccattt accggtggcc tgcaactcgc cgtcgggatt ggtgagtgcg
     6121 agcttgttga tgcgccactc gcgcccttcc ggtccgcgcg cattccttgc ctgcagcgtc
     6181 agccggccaa agcgcttgcc gaacaattcg aagttctcgg cgacgatgtc caccgccggc
     6241 aactgctcgg tactgccggg cgcctcgagc aggtcggcca cgccggactt ggccgcctcc
     6301 gggatgacga gcgaggtcag gcgggccgtg acccggccca tcccgcggcc ggagcgcggc
     6361 tcgttccagc tcaggtagcc ggagacctga tcggagtcga tgttcgcctg ccatacgcca
     6421 tcctgatggg aggcgccgac caccacattg ttcagcttgc ggtccatgat cagcagctcg
     6481 cgcgcctggg cggccagcac ttccggttcc acatattgct cgatcgacag cgcctggctg
     6541 cggcgcgcgc cgtcgccctg cggggcggga ccgagagcgg aagacatcgc gcgccgccat
     6601 gcgtccaggt ccaggctggg cacgttgacg ttcgcataca caccgctgtc cggctcgggc
     6661 gccggcgcat tcacgccgat gccgccgctg actacccgcc atgcagcctg gggagtggcc
     6721 ggcttctcgc gctcgtagtg cgcctggatc atcgtgccca gcgacagccg gatctggtcg
     6781 cgcagcagca gcgaatcgct ggccggcgac tgcaccatct cgaaacgcaa gggcaggctg
     6841 tcctgcgccg gtttgcgcag gggctcggga aaatccaggc cgaggccgac gaggttggac
     6901 tccaccatga ccgacggatt gccgtcgcgc acgcgcacgg tcgcaagata gcgggtgccg
     6961 ccggtgatgc ggtcggtcgc ccggctggtc atcggcatcg catacgcctg gcgcacgccg
     7021 gcggccgaca ttgcgccgtc ggcccgtacc agaatcgaac catcgcgctg cgtcccgccg
     7081 ttaagcgaga ccgggccgcc gaggaaattc gcgcgcacgc ccggcaatga aaatcccttt
     7141 tcattgaact ccagcgcacc cgaggcgccg gacagcggcg gcaggggctc gaacagatgc
     7201 acggtgttgc cggcaaactg cagcgtgccg cgcaccttcg catcttccat gtgcgccagc
     7261 ggaagctgca gctgcagggc aaggcgggcc gagccggtcg cgcgcgtggt ttcggtgaaa
     7321 ttgtcgatcc actggcccac cgggctgtcg tgggtgaagc gcacgaagtt ctgcaacggt
     7381 ccggccgctt cgccgtcgac ggtgagcaag ggctcgtcgg caagcaggtc cggaatcacc
     7441 actttcacgc gcgatagctg ggtacccgac gaacgcgcgc tgtcggcgaa gatgtccatg
     7501 cgattgcggt cgaacgcgat cctgccgttg atctggtcga tgatgggcca ttccggtttc
     7561 ttgccatcgg gggaatggcc ccagggcgcg taattcaggc tcccgttgac gatacggccg
     7621 ctgacgctga actccccctt gggcttgtcc ctggcggcca gcgtggcgaa aggaaactgc
     7681 gccaggtcgc ccttgacgcg gatgcgcaca tcttccgcat gaccacccag cagcgcaccg
     7741 ctcagccatt catgcagatg ctcgggcgtc tgcaacggca ggtagcgccg gacccggtcc
     7801 acctccagcc gggcgatctg gccgctcagg tcgatggtgc ccggcgattc tccgcccttc
     7861 gcgtcgagat tgagcacatg cttgccgctc agcgagccgc tgactccctc ctgcgcgaaa
     7921 tcgaagcgct gcaggtcgag cacgagctgg ttgcgatcct gaaagctcca gttcgcctgg
     7981 gccgcgaggc gctcaaacgg catcgctgcc tgcgcgaagt accccggcgt gtcgaggacc
     8041 acatcctcgg acgcgaggga cagcatgccg ccgcgctcgc tggcttcgat ccgcccggac
     8101 agattcgcaa agcccggaat cgccggcacg gccgcctgtc ccggcctgcc cgccgcgccg
     8161 gccacggcag gccgcgccgg ctgcgccttc agcgaaagcc cggtgaactg gccgcgtacg
     8221 ttataggcgg agaccgccgg atagacaccg tgccattgcg cggaaaagtc gcgcaccacg
     8281 ccgcgcggcg caagatcggc aagcagccgg cgctgatcgg caggcagcgg caggcgcgtg
     8341 gcaaagttcg ccagggtctc caggtccacc atgcgcgcct ggatctcggt acgcgccggt
     8401 cgcccgcgcg ccgccggcgt gtagctttcg ctgatggtcg tgcgcgggag gtaaagaccg
     8461 tcctcggtgc gcagcgagaa gtcggtcagc gcgaccgcat gtccgttggt gccgaaggtc
     8521 ggcgtgttgt cgtcgttgcc gggactgatt tcctcgcgca gcgcgatgcg cccgctgact
     8581 tccttgagcc gcagcggctc caggtccggc tgcaggcgcg ccaccacatt ggccagaccg
     8641 agatcggcgg tgaagtcggc caccttcgcg cgctcgaaat ccagccaggc gcgcaccgaa
     8701 ccgtggcccg actccatgtc gatcggataa tcgatccagg cgcgccatgc tgccagatcg
     8761 gtatcgcgca gatcgacata catctcgccc ttccatcggc tgacgtccga gatgcgccgc
     8821 gcgaacggcg gatgctcgaa gcgcgcgcgg atgtcgagcg gcgcggcgag cccgtccggc
     8881 ggctgcgcat gcagcgcaaa gcgatggcgg cgccagcggt tgtgcagcac gaagtcgacc
     8941 ttgtcgagca ccagttccgg cgcgccgcgc aggttgtcga tccagcgcag gcgccccccg
     9001 cggatcacga tttcccgctg cgacagcacc cagtccgcgc cccggccgtc ggactgctgc
     9061 ccggcggcca gctgtatgcc ggcgacatgg agccgtccct gggcgtcgcg cgcaatctcc
     9121 aggtccggcc tgtcgatctc caggctgttc aggcgcagtt cgccggcggc gatggtccac
     9181 cacgagagcg tggcggcgac cgacggcaag ctcagcgcag gcttgccgtc gtggccatga
     9241 atgacgacgg aatcgagcgc gaggcggggg tgcaggccgg accacgacgc gttcagcgca
     9301 ccgatcgtga caggattgcc gatcgcctcc gacgcgagtg cctcgatctg cggcttgtag
     9361 gcgtcgatgt tcggcaacac cacatagcga atgccgagca ccagcgcgca gaatgcaaaa
     9421 taggcaacaa tcgcagcagc gagaaggaac gagaacgtgc gcccgaagaa gtgatgcagg
     9481 atgcgccagc atccgcgcaa gatgtgccag cagcgcagga acaccgaagg cgccgctttc
     9541 tgtgcttgta cgggatcgga catcaaagag acaacggcgg cgtagaatag gtcgtcaaaa
     9601 aacttgcaga aggatagcac cggctcgtgc cgcgcatgct tgcacttcga aaaacggaat
     9661 cgtgcacgct cgtgcacgcg gcaccatcaa ccttcccgac accgtgactc catcccttac
     9721 gacaagccat attgcctcgc ggttctacgc tcgctggctc aatgcagatc cggcccgtgc
     9781 cggcatggtg gcggaactcg cgcgattatc gatcacgaaa gaccgcttcg gtttgctgtt
     9841 acaaagagaa accgatgccg gcatgccgct gcatcgtgcg atgcggcggg tacgcaatct
     9901 tgttgtttcg gccctgatcg agcgcgacct gtcgggacgc gcggacctcg acgaggtgct
     9961 ggaggcgatc agcgcgttcg ccgacttcgc ggtccagcgc catgtcgccg cgctcaccga
    10021 ggagatgcgt gcggcgcacg gcacgccggt cggcgccgag tcgggcgagc cgcaggaact
    10081 gatcgtgctg ggcatgggca agctgggcgg gcgcgagttg aacgtctcct ctgacatcga
    10141 cctgatcttc gtgtatccgg aagacgggga gacgcagacg actgcacccg gccagcggcc
    10201 gttgtcgaat catgaattct tcgcgcgcct tggcaagaag ctcatcgccg cgctgtcgga
    10261 gatcaccgag gacggcttca cgttccgggt tgacatggcc ttgcgcccga acggcgcctc
    10321 cgggccgctg gccgcaagct tcggcatggt cgaggaatac ctgatcgtgc agggacgcga
    10381 atgggaacgt tatgcgtggg tcaaggcgcg cgcgctgacc ggccggccgc aggacatcgc
    10441 cgcgctcgag cagatcgtgc agcccttcgt ctaccgccgc tacctcgact tcggcgcgat
    10501 cgaggccatg cgcaacatgc atgcgcagat ccgcgccgag gt
//
LOCUS       DATLGF010000515         3116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_124155, whole genome shotgun sequence.
ACCESSION   DATLGF010000515 DATLGF010000000
VERSION     DATLGF010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124155"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VM406_16070"
     CDS             <1..402
                     /locus_tag="VM406_16070"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007737187.1"
                     /note="Has polymerase, DNA-binding and 3'-5' exonuclease
                     activities. In Aeropyrum pernix this protein is sensitive
                     to aphidicolin and stable at 95#C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HVL77530.1"
                     /translation="ELYLRIFRGEPYRDYVRDYVDRTLAGGFDDRLVYRKRLRRTLAD
                     YQRNVPPHVRAARIADDYNRSQRRPLQYQNGGWIRYVMTVAGPEPLETRRAPIDYDHY
                     LTRQLQPVADAILPFVGDDFARLTSRQQILF"
     gene            497..835
                     /locus_tag="VM406_16075"
     CDS             497..835
                     /locus_tag="VM406_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HVL77531.1"
                     /translation="MKRLAILAMFVATQAAAHHGWSEYDASRPMELTGTIEGSGYEHP
                     HGYVRLKTADRTWTVVLAPPTRMENRGLSREMLGKGNRATVHGYPNRSKEDELRAEHI
                     TIGDKTVALR"
     gene            835..1323
                     /locus_tag="VM406_16080"
     CDS             835..1323
                     /locus_tag="VM406_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77532.1"
                     /translation="MEQDAGALAWLEATALSQAMRGSLWLYPIVEIVHIVGFVMLVGA
                     VAMFDVRLLGFSRSIPVRALARHLLPWSLAGLALIVPAGLMMFSAHPHDFVDSTVFRI
                     KLVLIALAGVNALLFHARAYRSVDAWNSMVAAPPAARVHALASIALWVAVISCGRLLA
                     YT"
     gene            1505..1963
                     /locus_tag="VM406_16085"
     CDS             1505..1963
                     /locus_tag="VM406_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVL77533.1"
                     /translation="MKRISEVMTPDVTVVRPDDKLQQAARIMRDEDIGALPVCDGKRL
                     VGMITDRDIAIRAVAEGKAADQCLVSEVMTNQVLWCYEDQNVGEVLQQMAHQQIRRIP
                     VINRNMELVGIVAIGDLATRQSQPVDAAMEDISAPFQPPPRTTGKQPSRH"
     gene            2137..>3116
                     /locus_tag="VM406_16090"
     CDS             2137..>3116
                     /locus_tag="VM406_16090"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HVL77534.1"
                     /translation="MLPSSGYGVTNATEALHQMQFERRDLNPSDVLIDILYCGVCHSD
                     LHTVRNEWGNTVYPVVPGHEIVGRVAAVGDQVSKFRVGDIAAVGCMVDSCQHCSACAE
                     GLEQYCENGFVGTYNGEEMHTGGMTYGGYARNIVVTEKFVLRVPGNLDPAATAPLLCA
                     GITTYSPLRQWKVGPGQKVGIVGLGGLGHMGIKIAHAMGAHVVLFTTSPGKAEDARRL
                     GADEVVVSRNAAEMKAHRNSFDFILNTVAAPHDLDAFLTLLKRDGTMTLVGAPPSPHP
                     SPQVFNLIMARRRLAGSLIGGIAETQEMLEFCAEHGIVADIEKIQIQEIN"
BASE COUNT          568 a         1029 c          996 g          523 t
ORIGIN      
        1 gagctgtatc tgcgcatctt caggggcgag ccctaccgcg actacgtgcg cgactacgtc
       61 gatcgcacgc tggccggcgg gttcgacgac aggctcgtgt accgcaagcg cttgcgccga
      121 acgctcgccg actaccagcg caacgtgccg ccgcatgtgc gcgccgcgcg catcgccgac
      181 gactacaacc gcagccagcg ccgtccactc caataccaga atggcggatg gatccgttat
      241 gtgatgacgg tggccggacc cgaaccactg gaaacgcggc gtgcgccgat cgactacgac
      301 cattacctga cacgccagct gcaaccggtg gccgacgcga tcctgccgtt cgtcggcgac
      361 gatttcgcgc ggctgacgag ccggcagcag atcctgttct gaacgattcg ccaaccgcca
      421 cgccacgccc cggtccgacg tagaatggtt tcatcctgcc cggattcatt gcctgcccac
      481 agcaacggag atgcgcatga aacgccttgc aatcctcgcg atgttcgtcg ccacacaggc
      541 cgcagcccac catgggtgga gcgagtacga cgccagccgg ccgatggagc tgaccggcac
      601 catagaaggt tccggttatg aacacccgca cggctacgtg cgcctgaaaa ccgccgaccg
      661 cacctggacc gtcgtcctcg ctccgccgac gcgcatggaa aaccgcggtc tgagccggga
      721 aatgctcggc aagggaaacc gcgccacggt gcacggctac ccgaaccgga gcaaggaaga
      781 cgagctgcgc gccgagcaca tcacgatcgg cgacaagacc gtagcactgc gctgatggag
      841 caggatgcgg gcgcgctggc ctggctggag gcgacggcgc tgtcgcaggc gatgcgcggc
      901 agcctgtggc tctacccgat cgtcgagatc gtgcacatcg tcggcttcgt gatgctggtt
      961 ggcgcggtgg cgatgttcga cgtccgcctg ctcggctttt cgcgctccat cccggtgcgc
     1021 gccctggcgc gccaccttct accgtggtcg ctcgccggcc tcgccctcat cgtgccggcg
     1081 gggctgatga tgttttccgc gcatccgcat gacttcgtcg acagcacggt gttccggatc
     1141 aagctcgtcc tcatcgcgct cgccggtgtg aacgcgctgc tgttccatgc gcgcgcgtac
     1201 cgctcggttg atgcatggaa cagcatggtc gccgcgccgc cggcagcgcg agtgcacgcg
     1261 ctggcttcca ttgcgctgtg ggtggcggtg atcagctgcg gacgactgct cgcctatacc
     1321 tgagcaaggg caaccatgtg tgctcgacgc gaccattgcc gccccgcttc gtcccggtgc
     1381 gcggcgcacc gcgcgcatcg ctgcgtcgct tgccgatatt caccctcgat gccggacagg
     1441 aaacttggca tccgtatgcc gctcaaatcc ttcccgcaga tagctacttt tccaggagac
     1501 gcacatgaaa aggatttcgg aagtgatgac gcctgatgtc acggtcgtca gaccggacga
     1561 caagttgcag caggccgcaa ggatcatgcg cgacgaagac atcggcgccc tgccggtgtg
     1621 cgacggcaag cgcctggtcg gcatgatcac cgaccgcgac atcgcgatac gcgcggtggc
     1681 ggaaggcaag gcggccgatc agtgcctggt ttcggaagtg atgacgaacc aggtcctgtg
     1741 gtgctacgag gaccagaacg tcggcgaggt gctgcagcag atggcgcacc agcagatccg
     1801 caggattccg gtgatcaatc gcaacatgga actggtcggg atagtggcga tcggcgacct
     1861 ggcgacgcgg cagtcccagc cggtcgatgc cgcgatggaa gacatctcgg caccgttcca
     1921 gccgccgccg cgcacaacag gcaagcaacc ctcgcggcac tgaggcttgc cggcgccgcg
     1981 tcgaaagggg ccgtttcgaa acatcggcac gccaggcgcg gcgcgtcgtc cggcgaatcg
     2041 cctggggacg gatggcgcaa agcttgcttg caatacgctg ttcgttcttc ggatttgcgc
     2101 gagttcgcaa tcccctcacc gctcaggagc aaaacgatgc tgccctcttc cggatacggc
     2161 gtgaccaatg cgaccgaggc gctgcaccag atgcagttcg agcggcggga cctgaacccg
     2221 agcgacgttc tgatcgacat cctctactgc ggcgtgtgcc actcggacct gcacacggta
     2281 cgcaatgaat ggggcaacac cgtctacccg gtcgtgcccg ggcacgaaat cgtcggccgc
     2341 gtcgccgcgg tgggcgacca ggtcagcaag ttcagggtcg gcgacatcgc cgcggtcgga
     2401 tgcatggtcg attcctgcca gcattgcagc gcctgcgcgg aaggcctgga gcagtactgc
     2461 gagaacggtt tcgtcggcac ttacaacggc gaggagatgc acaccggcgg catgacctac
     2521 ggcggctatg cgcgcaacat cgtggtgacc gaaaaattcg tgctgcgcgt tccgggcaac
     2581 ctcgatccgg ccgccaccgc gccgctgctg tgcgccggca tcaccaccta ctcgccgctg
     2641 cgccagtgga aggtcggccc gggccagaag gtgggcatcg tcggactcgg cggactggga
     2701 cacatgggga tcaagattgc ccatgcgatg ggcgcccacg tggtgctgtt caccacttcc
     2761 ccgggcaagg ctgaggatgc gcgccgactg ggcgcggatg aggtcgtggt ctcgcgcaac
     2821 gcggccgaga tgaaggcgca tcgcaattct ttcgatttca ttctcaatac cgtcgccgcc
     2881 ccgcatgatc tcgatgcctt cctcacgctg ctcaagcgcg acggaacgat gaccctggtc
     2941 ggcgccccgc cctcgccgca tccgtccccg caggtattca atctgatcat ggcgcgtcgg
     3001 cgccttgcgg gctcgctgat cggcggcatc gccgaaaccc aggaaatgct cgaattctgc
     3061 gcggagcacg gcatcgtggc cgacatcgag aagattcaga tccaggagat caacga
//
LOCUS       DATLGF010000516         3042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_220698, whole genome shotgun sequence.
ACCESSION   DATLGF010000516 DATLGF010000000
VERSION     DATLGF010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220698"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1855)
                     /gene="gcvP"
                     /locus_tag="VM406_16095"
     CDS             complement(<1..1855)
                     /gene="gcvP"
                     /locus_tag="VM406_16095"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HVL77535.1"
                     /translation="MVQKRAALHELEQHTDFIERHIGPDAQERRAMLAELGFESMDAF
                     ISRVIPASIVRDDALALGEAKSEPEVLDELRRIASKNKVYKSFIGMGYYDTHTPPVIL
                     RNLLENPAWYTAYTPYQPEISQGRLEALLNFQTMISDLTGMEIANASLLDEATAAAEA
                     MAFCQRVAKSKSRVFFVSKDCLPQTIDVVRTRAEPIGVEVVVGDHRADLGSQDCFGVL
                     LQYPAVDGEVVDYAATVEQVHAKGALVVVAADLLALTLLKPPGEFGADVAVGTAQRFG
                     VPLGYGGPHAAYFATRDANKRVMPGRVVGVSIDSHGDTAYRLALQTREQHIRREKATS
                     NVCTAQVLLANIASMYAVYHGPQGLKTIAERVHRLTATLVEGLRRLDIGVRAASFFDT
                     IVVRTGSRTADVHAAARSRMMNLREIDGENVGISLDETTTRADIEALWAVLGGSQALP
                     AFDACEAAAGTHIPAALVRTSAYLTHPVFNTHHSETQMLRYLRALADKDLALDRTMIP
                     LGSCTMKLNATTEMIPVTWKEFGAMHPFAPRDQAQGYQEMIAELEQMLATCTGYDAVS
                     LQPNAGSQGEYAGLLAIRAYHKSRGEGHRNVCLIPSSAHGTNPATAQMAGMQ"
     gene            complement(1947..2321)
                     /gene="gcvH"
                     /locus_tag="VM406_16100"
     CDS             complement(1947..2321)
                     /gene="gcvH"
                     /locus_tag="VM406_16100"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HVL77536.1"
                     /translation="MSTIHYTQEHEWLRVEDDGSVTVGITDYAQDHLGDLVYVQLPEP
                     GRAYAKGEEAAVIESVKTAGEILMPADGTVIEANGVLGDEPGKVNEDPMGAGWFFKFK
                     PAGDLGGFMDEAGYKAYIESLG"
     gene            complement(2598..2876)
                     /locus_tag="VM406_16105"
     CDS             complement(2598..2876)
                     /locus_tag="VM406_16105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77537.1"
                     /translation="MHLMTLGVLAVILIPLALYAQMQIGSHTRGAGRVALTRLLLLAV
                     GLAFGWVVSANAVEPAERFLVFVAGFGMVHVPAAVILFIKKRRGSGRT"
BASE COUNT          522 a          971 c          974 g          575 t
ORIGIN      
        1 cctgcatgcc ggccatctgg gccgtcgccg ggttggtgcc gtgcgcggag ctcgggatca
       61 ggcagacgtt gcggtgccct tccccgcggc tcttgtgata cgcgcggatc gcgagcaggc
      121 cggcgtattc gccttgcgag ccggcgttcg gctgcaggga gaccgcgtca tagccggtgc
      181 aggtggccag catctgctcc agctcggcga tcatttcctg gtagccctgc gcctgatcgc
      241 gcggcgcgaa cggatgcatc gcgccgaact ccttccaggt caccggaatc atctcggtgg
      301 ttgcgttgag cttcatcgtg caggagccca gcggaatcat cgtgcggtcg agtgcgagat
      361 ccttgtcggc cagcgcgcgc agatagcgca gcatctgggt ttccgagtgg tgcgtgttga
      421 ataccggatg cgtgagatag gcgctggtgc gcacgagcgc ggcaggaatg tgtgtgccgg
      481 ccgccgcctc gcaggcgtca aacgcgggca gtgcctggct gccgccgagc accgcccaca
      541 acgcctcgat gtcggcgcgc gtcgtggtct cgtccagcga gatgccgacg ttctcgccgt
      601 cgatctcgcg caggttcatc atgcggctgc gcgcggccgc atgcacgtcg gcggtacggc
      661 tgccggtgcg cacgacgatg gtgtcgaaga acgacgccgc gcgcacgccg atgtcgagac
      721 ggcgcaggcc ctcgacgagc gtggcggtca ggcggtgcac ccgctcggcg atcgtcttca
      781 gcccctgcgg tccatggtag acggcgtaca tgctggcaat gttggccagc agcacctggg
      841 cggtgcagac gttcgacgtc gctttctcgc ggcggatgtg ctgctcgcgc gtctgcaacg
      901 cgagacggta cgcggtgtcg ccgtggctgt cgatcgacac gccgacgacg cggccgggca
      961 tcacgcgctt gttcgcatcg cgcgtcgcga aataggccgc atgcgggccg ccgtagccaa
     1021 gcggcacgcc gaagcgctgc gcggtgccga cggccacgtc ggcaccgaac tcgcccggcg
     1081 gcttcagcag cgtcaaggcg agcagatcgg cggccaccac gaccagcgcg cccttcgcat
     1141 gcacctgttc gaccgtcgcc gcgtagtcga cgacctcgcc gtcgaccgcc ggatactgca
     1201 gcaacacgcc aaagcaatcc tgggagccaa ggtcggcccg atgatcgccg accacgacct
     1261 caacgccgat cggctcggcg cgcgtacgca cgacatcgat ggtctgcggc aggcaatcct
     1321 tcgatacgaa gaacacccgg ctcttgctct tggcgacgcg ctggcagaag gccatcgcct
     1381 cggcggcggc ggtcgcctcg tccagcaatg aggcgtttgc aatctccatg ccggtcaggt
     1441 ccgaaatcat ggtctggaag ttcagcagcg cttccaggcg tccctgcgaa atctccggct
     1501 ggtacggcgt ataggcggtg taccaggccg gattctccag caggttgcgc aggatgaccg
     1561 gcggcgtatg ggtgtcgtaa taccccatgc cgatgaagga cttgtagacc ttgttcttcg
     1621 acgcgatcct gcgcagttcg tccagcacct cgggctcgct cttcgcttcg cccagcgcca
     1681 gcgcatcgtc acgcacgatg gaagcgggaa tcacgcggct gatgaatgca tccatcgatt
     1741 cgaatccgag ttcggccagc atcgccctgc gctcttgcgc gtcggggccg atgtggcgct
     1801 cgatgaaatc ggtgtgctgc tccagttcgt gcaaagcagc gcgtttctga accatggtgt
     1861 cctctcgaag caaatgatga atgggccttt tggccggaac tgcgcaatgc gtgaagcggg
     1921 acccgcagtc ccgctgacga tgggcctcag cccagggatt cgatgtaggc cttgtaaccg
     1981 gcttcatcca tgaagccgcc gagatcgccg gccggcttga acttgaagaa ccagcccgct
     2041 cccattggat cttcgttgac cttgccaggt tcgtcgccga gcacgccgtt ggcctcgatt
     2101 accgtgccgt cggccggcat caggatttcg ccggcggtct tgaccgattc gatgaccgcc
     2161 gcctcctcgc ccttggcgta cgctcggccg ggctcgggca gctggacata gaccaggtca
     2221 cccaggtggt cctgcgcgta gtcggtgatg ccgaccgtga ccgagccgtc gtcttcgacg
     2281 cgcagccatt catgctcctg ggtgtaatgg atcgtgctca tgctttctct cctgtggcag
     2341 gttagttgtt gtttgtgttg gtactgcggc ccgcccgaat ggcggacttg tcgcccggcg
     2401 acgcggcctt tgagcccctg gctgtccagg gcgcgattgt agagcgatcg cgttttccgg
     2461 tgcattcaaa ccatcatgtc agattttcat ttcaaaaaca gaaatggcgc cgggtttgaa
     2521 aaaacaaaac tggcatggac tcgacttttg tttctaaaaa gcggacgact tcggcggttt
     2581 gcgccgctcc atacccctca tgtccgtccg gagccgcgtc gcttcttgat gaacaaaatg
     2641 accgccgcag ggacatgcac catgccgaat cccgccacga acacgaggaa gcgctcggcc
     2701 ggctcgaccg cattggcgga taccacccac ccgaacgcga gtccaaccgc gagcagcagc
     2761 agtcgcgtca gcgcgacgcg cccggccccg cgcgtatgac tgccgatctg catctgggca
     2821 tacaaggcca aggggatcag gatgacagca agtacgccta gcgtcatgag atgcatgact
     2881 ccctccatca cagtgtcgat gcgatatcga ttgctctttc gcagaagagg ttgcatcccg
     2941 tcgaacgttc gggtcgacac caggacctat ccgataggtg gatccctgga gaggttcaga
     3001 tggcgggaac aaggccgcgg cgaacaggca tattgcttgc gc
//
LOCUS       DATLGF010000517        13976 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_205620, whole genome shotgun sequence.
ACCESSION   DATLGF010000517 DATLGF010000000
VERSION     DATLGF010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 13976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13976
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205620"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="VM406_16110"
     CDS             complement(<1..933)
                     /locus_tag="VM406_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010107262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HVL77538.1"
                     /translation="MSKLFSPISLGKLELANRIAIAPMCQYSARDGMATNWHLIHLGH
                     LAHSGAGILFLEATAVEPEGRISAADLGLWSDATEQALGQVLRAVREHSAMPIAIQLA
                     HAGRKASTRVPWEGGHSVPSSEGGWRTVAPSALPFLEQDETPLALDNNGLQRVRAAFA
                     DAARRADRLGIDAIEVHAAHGYLLHQFLSPLANRREDGYGGTLENRMRFPLEVFDAVR
                     ASFNANKPVGVRISATDWVEGGWDVEQSAAFAQELKKRGCDFIDVSSGGVSPAQQIKL
                     GPGYQVPFAERIRAAAGMPTIAVGLITEAEQAQQI"
     gene            1054..1317
                     /locus_tag="VM406_16115"
     CDS             1054..1317
                     /locus_tag="VM406_16115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77539.1"
                     /translation="MTKRKMPEDDMQEPTSSQGEDSKSVTPKDVGSSTASAKDVKERK
                     VASDDPEERIEEQLDDAVEMTFPASDPIAIPDPDKAERHHPRP"
     gene            complement(1333..2409)
                     /locus_tag="VM406_16120"
     CDS             complement(1333..2409)
                     /locus_tag="VM406_16120"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HVL77540.1"
                     /translation="MTHFNVENLNVTSFNAMPTPEDLHARLPLSEAAGVTVTRGREAL
                     RNILDRRDPRLFVVVGPCSIHDPVAGLDYARRLKTLQEEVQDTLLLVMRVYFEKPRTT
                     TGWKGFINDPDMDDSFRVDRGMELARRFLLDVCELGLPTATEALDPISPQYLGDLIAW
                     TAIGARTTESQTHREMSSGLSTPVGFKNGTDGDIGIAINAILSASQPHAFLGINGQGR
                     VSIVRTSGNRYGHVVLRGGDGRPNYDTVSVAMAEQALAKAGIAANIVVDCSHANSFKN
                     PELQPLVMADVVNQVRLGNTSLAGVMIESNIVAGRQAIPEDLSQLKYGCSVTDGCVGW
                     ETTQKMIRDADAMLRGVLGARRQR"
     gene            2600..4837
                     /locus_tag="VM406_16125"
     CDS             2600..4837
                     /locus_tag="VM406_16125"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HVL77541.1"
                     /translation="MNPKSRTASPPSFASRGARRADVLDSLVQLASRQANAQIDAFSA
                     RLANALLAQSQASRDADEARLSFMYGNLLKENQYAFFHLASQRLEERLRAAVDALHDK
                     GVAAPVVEKAELSLVPYEEMEQEVLLGSASRPYEAENEAVLDTLNRRLAVLLERDVLA
                     SRSNPFRPEVFLDAIVATWTEFLPEGGPDFPIMPLLRPELFLDLGEILRALDAALAAR
                     GVEDHAPHGGAIRKARQAGVQADADPAALSNQLKRLFALDAPPASAAPGPAFAPAGSD
                     LMPQLPQPALPVSAQLLGMLADLQRQGGWAQRPAFAPAGEGHARMPIDDPVQPESGAE
                     QPAPPAGQRSQLRQLKRQLPHGALSRHDETTIDLLGTVFDTIFEDPHIPSEMKDLIGL
                     LQIPVLKAALIEKEFFYQEAHPARRLIELLSRSSIGWDSSKGRDDPLYQAIKRNVDRV
                     QQDFAHELGVFSDVLSDLEAHIEREEAAASEALAAPINQALKTERMRTATKAAENDVA
                     VRLSTGEVATFVEAFLEKRWVPILALAYTVKDEKPQLLAHAVQTMDDLIWSVKPKVTA
                     EERKELIGKLPELLAALNKWIGLMKWDDAERMQFFADLAECHASIVRAPLELSPQRQV
                     EVAVEAAKRAAEKRAAQRANLPEPVLDEHVETVETLERGVWLNFTQPDGGAKKVKLAW
                     VSPLRSLYIFSTSSKEEAFSLSAEALAQAFREGRAGIVVADRFVDRALSRALAGQGAE
                     VALAV"
     gene            complement(5128..5955)
                     /gene="nudC"
                     /locus_tag="VM406_16130"
     CDS             complement(5128..5955)
                     /gene="nudC"
                     /locus_tag="VM406_16130"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HVL77542.1"
                     /translation="MLRTPDSFLALLEPHSDPAPATFIFRDGELLVRDEDLALPSDPA
                     LALRDWDATLHAVGLLDEKYCRAAWVARSTEPAPGYSFRGLRALFGAIDDAVLGVAGR
                     AFQIVEWARTHRYCGVCGTANETVAGERCLRCPSCGHGAYPRISPAMMVLVRRDDHIL
                     LARHAGPRAVRFSPLAGFLEPGESIEEAVHREVMEEVGLKVTNLRYFGSQSWPFPHSL
                     MIAFTADYAGGEIRLQEDEIVEARWFGPQDEMPPYAQGISISGALIEANWPGRRRPE"
     gene            complement(6001..7269)
                     /locus_tag="VM406_16135"
     CDS             complement(6001..7269)
                     /locus_tag="VM406_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVL77543.1"
                     /translation="MKRFLLALLTVAVLAIGLVLWLGLREDDSHPSTTPDVASAELVA
                     RGAYLARAGNCIGCHTARGGQPYAGGRAIQTPFGAIYASNITPDRETGIGAWSADDFW
                     RALHNGKSRDGSLLYPAFPFPNYSRVTRADSDAIYAYLQSVPAVRQINRAPELDFPFN
                     QRWLLVGWRALYFRPEVYQPQPAQSAEWNRGAYLVQGLGHCSACHTARNVFGAQRQEL
                     SGGIIPMVNWYATSLTGEHGAREWPLEDLIAFMGTGVSMQGSASGPMAEVVRDSLQHL
                     QDDDLHAIAVYLKSLPPDEVTPSAEQPLPGPEHKAFLQRGARVYEKHCVDCHGADGRG
                     EPPHFPPLAGNRSVLVPVPVNPIRLLVHGGYPPSTEGNPYPFGMPPFGNLLTDEEAAA
                     VLSYIRNAWGNRASVVASPQINRLRPVMID"
     gene            complement(7266..7895)
                     /locus_tag="VM406_16140"
     CDS             complement(7266..7895)
                     /locus_tag="VM406_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HVL77544.1"
                     /translation="MEKRVAPCMACHGEQGRAAPDGYYPRIAGKPQGYLYNQLVNFRD
                     GRRQQYPLMIYMVQHLSDDYLMEMARFFAEQDLPYPPPQPVPANAGVMERGRQLAVQG
                     DAARRIPACVACHGAKLTGVAPSVPGVLGLPRDYLVAQFGAWRIGTRRAHAPDCMGQV
                     AREMSAEDINAVSTWLAAQPLPAEPKAEGSFPAPLPLACGSVGQGGQPR"
     gene            8178..8675
                     /locus_tag="VM406_16145"
     CDS             8178..8675
                     /locus_tag="VM406_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVL77545.1"
                     /translation="MSSLQRISPCLWFDDQAEEAARLYTSIFPNSRIGRVTRYGEAGR
                     EFHRKPPGSVMTVEFVLDGQPFTALNGGPVFKFNEAISFQIDCEDQGEVDFYWARLGE
                     GGDPAAQQCGWLKDRFGVSWQVVPAMLPELLDGGDNAAGQRVMQALLKMKKLDIETLR
                     RAREA"
     gene            8796..9824
                     /locus_tag="VM406_16150"
     CDS             8796..9824
                     /locus_tag="VM406_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HVL77546.1"
                     /translation="MHIYFSDEHRLHKGEMEFFRGELVPCFEKPERADFVLAALTEHA
                     IGPVHAPDAFPLEAIERVHAPRYLRFLERAWGMWSALGQTRDAIPAVWPIRGFRDDVE
                     PDNFIAQLGLYSYDSGSPFTAGTWQAARRAADCALAAQAAIAGGERAAFALARPPGHH
                     AGPDFFGGYCFINNAAVAAQAFRDQGAGRVAILDVDYHHGNGTQSIFYRRADVFTQSI
                     HGDPRTEYPFYLGHADETGEGEGDGFNANYPLPAGSSNALWFEALGAATARIRAYAPD
                     ALVVSLGVDTYAGDPISKFRLDQPEFLRIGKEIAALGLPTLFVMEGGYAVAEIGYNVA
                     AVLQGFQD"
     gene            9867..10511
                     /locus_tag="VM406_16155"
     CDS             9867..10511
                     /locus_tag="VM406_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HVL77547.1"
                     /translation="MYRIPTIMLDFETTGLSPAAGARITEVAALRIENGRIVDRFVSL
                     VNCCVDVPPFITALTGITQQMVDRAPCVSQVVPELLRFIGEDALAAHNASFDEKFLLA
                     ECAMLGTAPRHAHLLCSLKLSRRLLPGLRSYGLSRLSSSLGIAFSGDAHRAEADAEVS
                     AKVLLHIGEQLRRSYGFDDVGPDLLARVGSLSAARVPAFLEREAGQRTSAPHAC"
     gene            10712..12199
                     /locus_tag="VM406_16160"
     CDS             10712..12199
                     /locus_tag="VM406_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77548.1"
                     /translation="MIKPPLRGLTAAARSPFVLAILLAASTAAAAPVSLPSYNVDASR
                     TSVSGLSSGGFMAAQLHVAYSATFKAGAGIVAGGPYYCAQGSLTTATGPCMAATTWSK
                     PATSTLISTTNSWASQGVIDPTSNLTNSRVYLFSGSLDSTVRPLVMDEANTYYRNYVN
                     HANVFYKKDIAAEHAMVTDYFGNSCSTKASPYINNCNFDLAGEILKWIYGSLNAKNNG
                     SLGGSFIEFNQAEFISNPTSRGMANTGWMYVPANCAAGQSCRLHVALHGCQQDPTKIQ
                     DRYYRNTGYNKWADTNNIIVLYPQTAPSSTGNPNGCWDWWGYDDPNYAKKSGRQMLAI
                     KGMVDRITTGGAPAPGAPTNLAVTGTTANSVSLSWNAVSGASGYHVYRNGTRVTSSAV
                     AATSYTDSGLASGTTYSYAVSAMVSGTESALSATVSATTTSSGHACTQTTSSNYTHVT
                     AGRAYNSGGYALAKGSNQNMGLNNTFYTTTLAQTAPGYYVIGNCP"
     gene            12243..12758
                     /locus_tag="VM406_16165"
     CDS             12243..12758
                     /locus_tag="VM406_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77549.1"
                     /translation="MKTASLAVALSAAALCAWGTWDQLATRHEPVRLLARPATGLAPV
                     PAVGAAALPHVGEGSLVLFERDASGALRIGRHTRERLEELARSLPYDLSHAEWQAIDE
                     FAAAGLDEPAASQARRIVREHLAARQAPSPGRAAPATAHPEPMTESLLALRPTPPPEQ
                     AVMRFPGMGDE"
     gene            12780..>13976
                     /locus_tag="VM406_16170"
     CDS             12780..>13976
                     /locus_tag="VM406_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HVL77550.1"
                     /translation="MKRFKHSTIASAAMLALMASGAASAVVTLPRYNVSFDNVSVSGI
                     SSGGYMAAQFHFAYSKTVKKGAGVIAGGPVYCSQGNVNIATGPCMADTGSRNLPYLIS
                     VVNTWSGNGYIDPTSNLANAKVYLFSGTIDSTVKQPVMNDLRTMYGNYVSSANITYKN
                     NLAAEHAIVTDYYGSACSYKGSPYINNCNYDTAGEILKWIYGALNAKNTGTLGGSFVN
                     FNQRHYWGNADPTNHGMANDGWAYVPANCAAGQPCRLHVAFHGCQQNAATIGDKFYRN
                     TGYNRWADTNNIIVLYPQAHATFANPNGCWDWWGYDDVNFPGKSGGQMVAIKTMIDRI
                     VSGNAAAPYTCSSWYASNYSHVVNGRAYASNGNAYAVNSNQYLGYYSTAAYTNVRRTS
                     AGYYAYG"
BASE COUNT         2449 a         4664 c         4583 g         2280 t
ORIGIN      
        1 gatctgctgc gcctgttcgg cttcggtgat cagtcctacc gcgattgtcg gcatgcccgc
       61 cgccgcgcgg atgcgttcgg cgaacggcac ctggtagccg gggccgagct tgatctgctg
      121 ggccggcgat acgccgcccg atgagacgtc gatgaagtcg caaccgcgct tcttcagctc
      181 ctgcgcgaat gccgcgcttt gctcgacatc ccacccgcct tcgacccagt cggtcgccga
      241 gatgcgcaca ccgaccggtt tgttcgcgtt gaagctggcg cgcaccgcgt cgaatacctc
      301 gagcggaaag cgcatgcggt tctccagcgt gccgccgtag ccgtcctcgc ggcggttcgc
      361 aagcggcgac aggaactggt gcagcaggta gccgtgcgcg gcatgcacct cgatggcgtc
      421 gatgccgagg cggtcggccc gccgtgccgc gtcggcgaag gcggcgcgca cgcgctgcag
      481 gccgttgttg tcgagcgcca gcggcgtctc gtcctgttcg aggaagggca gggcggaggg
      541 cgccacagtg cgccagccgc cttcggacga cggcaccgag tgcccgcctt cccaaggcac
      601 ccgggtcgaa gccttgcgcc cggcgtgggc cagctggatg gcaatcggca tcgccgagtg
      661 ttcgcgcacc gcgcgcaaca cctggccaag ggcttgctcg gtcgcgtcgg accacagccc
      721 gaggtcggcg gccgaaatgc ggccttccgg ctcgaccgcg gtcgcctcca ggaacaggat
      781 gccggcgccg gagtgcgcca gatggccgag atggatcagg tgccagttgg tggccatgcc
      841 gtcgcgcgcc gaatactggc acatcggtgc gatggcaatg cggttggcaa gctcaagctt
      901 gccgagggag atcggggaga acagtttgct catggtcgta tcgttcttgg gacagacgat
      961 ttaaccactt ttgagatttc cctgcccggg ggaacccgcg cgcacaagca gccgtcttag
     1021 caacactacg actacacgct cacggaggaa gtaatgacca agcgcaagat gcccgaagac
     1081 gatatgcagg agccgacttc gtcgcagggc gaggattcta agtctgtgac gccgaaggat
     1141 gtcggctcgt cgactgcttc ggccaaggac gtaaaggaac gcaaggtcgc atcggacgat
     1201 ccggaagaac ggatcgagga acagctcgac gatgcggtcg agatgacctt cccggcgagc
     1261 gatccgatcg ccattccgga tccggacaag gccgagcggc accaccctcg gccgtgatgc
     1321 gtgcacacgg agttagcgct ggcggcgtgc cccaagcacg ccgcgcagca tcgcatcggc
     1381 gtcgcggatc atcttctgcg tcgtctccca ccccacgcag ccgtcggtga ccgaacagcc
     1441 gtacttcagc tgcgacaaat cttccggaat cgcctgcctg ccggccacga tgttcgactc
     1501 gatcatcacg ccggccagcg acgtgttgcc aaggcggacc tgattgacca cgtcggccat
     1561 caccagtggc tgcagctccg gattcttgaa actgttcgcg tgcgagcagt cgaccacgat
     1621 gttggccgca atgccggctt tcgccagcgc ctgctcggcc atcgccaccg ataccgtgtc
     1681 gtagttcggc cgcccgtcgc cgccgcgtag cacgacatgg ccgtagcgat tgccgctggt
     1741 gcgcacgatc gacacgcgtc cctgcccgtt gatgccaagg aaggcgtgcg gctgcgaggc
     1801 cgacaggatc gcattgatcg cgatgccgat atcgccgtcg gtgccgttct tgaagccgac
     1861 cggcgtcgac aggccggacg acatctcgcg atgggtctgg gactcggtcg tgcgcgcgcc
     1921 gatcgcggtc caggcgatca ggtcgccgag atactgcggc gagatcgggt ccagcgcctc
     1981 ggtggccgtg ggcaggccca gctcgcagac gtcgagcagg aagcggcgtg ccagttccat
     2041 gccgcggtcg acgcggaacg agtcatccat gtccggatcg ttgatgaatc ccttccagcc
     2101 ggtggtcgtg cgcggcttct cgaagtacac gcgcatcacc agcagcagcg tgtcctgcac
     2161 ctcttcctgc agcgtcttca ggcgccgcgc gtaatccagc ccggcgaccg gatcgtggat
     2221 cgagcacggc ccgactacga cgaaaaggcg cgggtcgcgt cggtcgagaa tgttgcgcag
     2281 tgcttcgcgg ccccgggtga cggttacgcc ggcggcttcc gacagcggca gccgggcatg
     2341 caggtcttcc ggcgtgggca tcgcgttgaa tgaggtgacg ttgaggtttt cgacgttgaa
     2401 gtgggtcatg acgaactgcg cggcggctgg atggaaagga atgcaacagt gtaccggctg
     2461 ccgcggcaaa tcgccccgac gcgtcggtcc tagaaattag ttgccatacg gaaataagtg
     2521 catacaatga agcatcgtgc cctcgccgcg aggcgggtac gcgaaccctt ctgcatccgg
     2581 ccagtccgcc cgtacgcgca tgaacccgaa atctcgcacg gcttctcccc cgtctttcgc
     2641 atcgcgcggc gcgcggcgcg cggacgtgct ggacagcctc gttcagctgg cgtcgcggca
     2701 ggccaacgcg cagatcgatg cattttcggc acgcctcgcc aacgctctgc tggcgcagtc
     2761 gcaggcgagc cgcgacgccg acgaagcgcg cctgtccttc atgtacggca atctgctgaa
     2821 ggagaaccag tacgcgttct tccacctcgc atcgcaacgg ctggaagaac gtttgcgtgc
     2881 cgcggtcgat gcgctgcacg acaagggcgt ggcggctccg gtcgtggaaa aggcggaact
     2941 gtcgctggtg ccctacgagg agatggagca ggaggtcctg ctgggcagtg ccagccggcc
     3001 gtatgaagcc gaaaacgaag ccgtgctgga cacgctgaac cggcgtctgg cggtgttgct
     3061 ggagcgcgac gtgctggcgt cgcgcagcaa cccgttccgg cccgaggtct tcctcgacgc
     3121 gatcgtcgcc acctggaccg aattcctgcc cgagggcggg ccggatttcc cgatcatgcc
     3181 gctgctgcgg cccgaactgt ttctcgatct tggagagatc ctgcgcgcgc tggatgccgc
     3241 gctggcggcc aggggcgtcg aggaccatgc cccgcatggc ggcgcgatcc ggaaggcgcg
     3301 gcaggcgggc gtccaggctg atgccgatcc cgctgcgctg tccaatcagc tcaagcgcct
     3361 gttcgcgctg gacgcgccac ccgcatccgc ggctccgggg cccgccttcg cgccggccgg
     3421 aagcgacctg atgccccaac tgccgcagcc ggcgctgccg gtcagtgcgc aattgctcgg
     3481 catgcttgca gacctgcagc ggcagggcgg ctgggcccag cgtccggcct tcgcccccgc
     3541 tggcgaggga catgcgcgga tgccgatcga cgatcccgta cagcctgaaa gcggcgcgga
     3601 gcagcctgcg ccacccgccg ggcagcgttc gcagctcagg cagctcaagc gacaattgcc
     3661 gcacggcgcg ctgtcccgcc atgacgaaac cacgatcgat ctgctcggca ccgtcttcga
     3721 caccatcttc gaggacccgc acatcccttc cgagatgaag gatctgatcg ggctgctgca
     3781 gattccggtg ctcaaggccg cgctgatcga gaaggaattc ttctaccagg aagcccatcc
     3841 ggcgcggcgc ctgatcgagc tattgagccg gtcgagcata ggctgggaca gcagcaaggg
     3901 acgcgacgat ccgctgtacc aggcgatcaa gcgcaatgtg gaccgtgtcc agcaggattt
     3961 cgcgcacgag ctgggcgtgt tctcagacgt gctctcggac ctggaagcgc atatcgaaag
     4021 ggaagaggct gccgcgtccg aggcgctcgc cgcgccgatc aaccaggcct tgaagaccga
     4081 gcgcatgcgt acggcgacga aggccgcgga aaacgacgtg gcggtgcgtc tgtcgaccgg
     4141 cgaagtggcg accttcgtcg aggccttcct cgagaaacgc tgggtgccca tccttgcgct
     4201 ggcctatacg gtgaaggacg aaaagccgca actgctcgcg catgcggtgc agaccatgga
     4261 cgacctgatc tggagcgtga agccgaaggt cacggccgag gaacgcaagg agctgatcgg
     4321 caagcttccg gaactgctcg ccgcgctcaa caaatggatc ggtctgatga aatgggacga
     4381 tgccgaacgc atgcagttct tcgccgacct ggccgaatgc cacgcctcta tcgtgcgcgc
     4441 gccgctcgaa ctgtcgccgc aacgccaggt cgaggtggca gtggaggcgg ccaaacgcgc
     4501 tgccgagaaa cgcgcggcgc agcgcgccaa ccttcctgag ccggtgctcg acgagcatgt
     4561 cgagacggtc gagacgctcg agcgcggcgt ctggctgaat ttcacccagc ccgacggcgg
     4621 cgcaaagaag gtcaagctgg cttgggtcag cccgctgcgc agcctgtaca ttttctccac
     4681 cagcagcaag gaagaggcct tctcgctatc ggccgaggcg ctcgcccagg cctttcgcga
     4741 gggccgcgcg ggcatcgtcg tggccgaccg tttcgtcgac cgcgcgctgt cgcgtgcgct
     4801 tgccgggcag ggcgccgagg tggcgctggc ggtgtagcgc gattgcggga ttgagcggtc
     4861 tgcctcggag gcgccgccaa cttgtctgct cgcggcgctc ccggacgatt caggaatcgc
     4921 ttcatgaacc gtttcacgaa tcgtttcacg aatcgtttca cgaaaaggcg caggcaggcg
     4981 gttttccccg ttgattcctc gttccggaat cgtttcacct aacagtgcaa ggattcaggt
     5041 cgtagccggg cagcctaagg ccgcattgcg tgaaaccggc acttcatcac gctgacaacc
     5101 cgcttcgcgc aaaatacacg cgccgcacta ttccggcctg cgccggcccg gccagttggc
     5161 ctcgatcagc gcgccggaaa tcgaaatccc ttgcgcgtac ggcggcatct cgtcctgcgg
     5221 gccgaaccag cgcgcctcga cgatttcgtc ctcctgcagg cggatctcgc cgcccgcgta
     5281 gtccgcggtg aacgcgatca tcagcgagtg cggaaacggc caggactggc tgccgaagta
     5341 gcgcaggttg gtgaccttca ggccgacctc ttccatcacc tcccggtgca ccgcctcctc
     5401 gattgattcg ccgggctcaa ggaagccggc cagcggggaa aagcgcaccg cgcgcgggcc
     5461 ggcatggcgc gcgagcagga tatggtcgtc gcgccgcacc agcaccatca ttgcgggcga
     5521 gatgcgcggg taggccccat gtccgcagga cgggcagcgc aggcagcgtt cgccggccac
     5581 cgtctcgttc gccgtgccgc acacgccgca atagcgatgt gtgcgcgccc attcgacgat
     5641 ctggaacgcg cggccggcca cgccgagcac cgcgtcgtcg atggcgccga acagcgcgcg
     5701 caggccgcgg aagctgtagc cgggcgccgg ctcggtggag cgcgccaccc aggcggcgcg
     5761 gcagtacttc tcgtcgagca ggccgaccgc atgcaaggtg gcatcccagt cgcgcagcgc
     5821 cagcgcggga tcggacggca aggccaggtc ttcgtcgcgc accagcagtt cgccgtcgcg
     5881 gaagatgaac gtggccggcg ccggatcgct gtgcggttcc agcagggcaa gaaaggagtc
     5941 gggcgtgcgc agcatgggga attccggtaa tggccgatgg ctgactatag cagcgccatg
     6001 tcagtcgatc attaccggcc gcaagcggtt gatctgcggg ctggcgacca ccgacgcccg
     6061 gttgccccag gcattgcgga tgtagctgag cactgcggcg gcctcctcgt cggtcagcag
     6121 gttgccgaac ggcggcatgc cgaacgggta ggggttgcct tcggtgctgg gcggatagcc
     6181 gccatgcacc agcaggcgga tcggattgac cggcaccggc accagcaccg agcggttgcc
     6241 cgccagcggc gggaaatgcg gcggttcgcc gcggccgtcg gcgccgtggc agtcgacgca
     6301 gtgcttctcg tacacgcgcg cgccacgctg caggaaggcc ttgtgttccg gtccgggcag
     6361 cggctgctca gcggacggcg tcacctcgtc cggcggcagg ctcttcaggt agaccgcgat
     6421 cgcgtgcagg tcgtcgtcct gcaggtgctg caggctgtcg cgcaccacct ccgccatcgg
     6481 acccgaggcc gaaccctgca tcgacacgcc cgtgcccatg aaggcgatca gatcctccag
     6541 cggccattct cgcgcgccgt gctcgccggt tagcgaggtc gcataccagt tcaccatcgg
     6601 aatgatgccg ccgctcagtt cctggcgctg cgcgccgaac acgttgcgtg ccgtatggca
     6661 ggcgctgcaa tggccgagtc cctgcaccag ataggcgccg cggttccact cggcgctctg
     6721 cgccggctgc ggctggtaga cctcgggtcg gaaatacagt gcgcgccagc cgaccagcag
     6781 ccagcgctgg ttgaacggga aatccagttc cggcgcgcgg ttgatctggc gcaccgccgg
     6841 gacgctctgc aggtaggcgt agatcgcatc cgagtcggca cgcgtgacgc ggctgtagtt
     6901 cgggaacggg aaggccggat acagcagcga gccgtcgcgc gacttgccgt tgtgcagcgc
     6961 gcgccagaag tcgtcggcac tccatgcgcc gatgccggtc tcgcggtccg gcgtgatgtt
     7021 ggatgcatag atcgcgccga acggtgtctg gattgcgcgc ccgcctgcat acggctggcc
     7081 gccgcgcgcg gtgtggcagc cgatgcagtt gccggcgcgc gcaaggtagg cgccgcgcgc
     7141 gaccagctcc gcgctggcta cgtccggcgt ggtggacgga tggctgtcgt cttcgcgcag
     7201 gccgagccac agcaccaggc cgattgccag gacggcgacc gtcagcagag cgagcaggaa
     7261 gcgtttcatc gcggctgtcc tccctggccg acactgccgc aggccagcgg caggggcgcg
     7321 ggaaagctgc cctcggcctt tggctcggcg ggcagcggct gcgccgcgag ccaggtcgag
     7381 accgcattga tgtcctccgc gctcatttcg cgcgcaacct ggcccatgca gtcgggcgcg
     7441 tgcgcgcgcc gtgtaccgat gcgccaggct ccgaactggg cgacgaggta gtcgcgcggc
     7501 aggccgagca cgccgggaac cgagggcgcc acgccggtca gtttcgcgcc gtggcaggcc
     7561 acgcaggcgg ggatgcggcg cgcggcatca ccctgcacgg caagctggcg gccgcgttcc
     7621 atcacgccgg cattggcggg cacgggctgc ggcggcggat agggcaggtc ctgctcggcg
     7681 aagaagcgcg ccatttccat caggtagtca tccgacaggt gctgcaccat gtagatcatc
     7741 agcggatatt gctggcgccg gccgtcgcgg aaattcacca gctggttgta caggtagcct
     7801 tgcggcttgc cggcgatgcg cgggtagtag ccgtccggcg cggcgcggcc ctgctcgcca
     7861 tggcaggcca tgcacggcgc gacgcgcttt tccatcgctt cgatgcgggc ggacgccgtg
     7921 gcgggcgcaa ttgacaggac gatcagcagc ggccagatgg acggcaggcg cttggggggc
     7981 gtgaggcggg gaatgaatcg catggcaagg ccgatatgac aagcccatga ttatccggcg
     8041 aaacacgaga cgctgcaggt cgcatcggct tgcgcgcgcg cccgccatat gagttcgcac
     8101 aaaatccgtt ccggaatgtc gatgtgcgtg gccagtggac gactacgctg catcagtgac
     8161 aagaaaggag atgcgcaatg tccagcctgc aacggatttc cccctgcctg tggttcgacg
     8221 accaggccga ggaggcggcc cgcctctata cctcgatctt tccgaattcc cgcatcggcc
     8281 gcgtcacccg ctatggcgag gcggggcgcg aattccaccg caagccgccc ggctcggtga
     8341 tgacggtcga gttcgtgctc gacggccagc ctttcaccgc cctgaacggc ggaccggtgt
     8401 tcaagttcaa tgaagcgatc tcgttccaga tcgactgcga ggaccagggc gaggtcgatt
     8461 tttactgggc aaggctgggc gagggcggcg atcccgctgc ccagcagtgc ggctggctga
     8521 aagaccgctt cggcgtgtcg tggcaggtgg tgccggccat gctgcccgaa ctgctcgatg
     8581 gcggcgacaa tgcggccggc cagcgcgtga tgcaggcgct gctcaagatg aagaagctgg
     8641 atatcgagac gctgcgccgg gcacgcgagg cgtagtgcgc aagcccttgc gcgccggcgc
     8701 ccgggccgac gttgactcgc agcaaaaggc ggtgttgcca agtcggaagt gctggcgtac
     8761 tgtcatcagc ttaccttcct gacaggcggc cgaccatgca catctacttc agcgacgaac
     8821 accgcctcca caagggcgag atggaattct tccgcggcga actggtgcca tgcttcgaaa
     8881 agcccgagcg cgccgatttc gtgctggccg cgctcaccga gcatgcgatc gggccggtgc
     8941 atgcgcccga tgccttcccg ctcgaggcga tcgagcgtgt gcatgcgccg cgctacctgc
     9001 gcttcctcga gcgcgcctgg ggcatgtgga gcgcgctcgg gcagacgcgc gatgcgattc
     9061 ccgcggtgtg gccgatccgc ggctttcgcg acgatgtcga gccggacaac ttcatcgccc
     9121 agctcggcct gtattcgtat gacagcggat cgccgttcac cgcaggcacc tggcaggccg
     9181 cgcgccgcgc ggccgactgc gcgctcgcgg cgcaggcggc gattgccggg ggcgagcggg
     9241 ccgcgttcgc actcgcgcgc ccgcccggcc accatgccgg ccccgacttc tttggcggct
     9301 actgcttcat caacaatgcc gccgtcgccg cccaggcctt tcgcgaccag ggcgccggcc
     9361 gcgtggcgat cctcgatgtc gattaccacc acggcaacgg cacccagtcg attttctacc
     9421 ggcgcgccga cgtcttcacg cagtcgatcc acggcgaccc gcgtaccgag tacccgttct
     9481 atctcggcca tgccgacgaa accggggagg gggagggcga cggcttcaac gccaactatc
     9541 cgctgccggc gggcagcagc aacgcgctct ggttcgaagc gctcggcgcg gcgaccgcgc
     9601 gcatccgcgc ctacgcgccg gacgcgctgg tggtgtcgct tggcgtggac acttatgccg
     9661 gcgacccgat ttcgaagttc cgcctggacc agccggagtt cctgcgcatc ggcaaggaaa
     9721 tcgccgctct cggtctgccc acgctgttcg tgatggaggg cggctatgcg gttgcggaga
     9781 tcgggtacaa cgtcgcggcg gtgttgcaag gttttcagga ctagcaatgc tgcacacggc
     9841 aactgcgaac gccgtagaat cgccccatgt atcgcattcc taccatcatg ctcgacttcg
     9901 agaccaccgg cctgtcgccc gcggccggtg cgcgcatcac cgaagttgcg gccctgcgca
     9961 tcgagaacgg gcgcatcgtc gaccgcttcg tttccctggt caattgctgc gtcgacgtgc
    10021 cgcccttcat caccgcgctg accggcatca cccagcagat ggtcgaccgc gcgccctgcg
    10081 tgtcgcaggt tgtgccggag ctgctgcgct tcattggcga agatgcgctg gccgcgcaca
    10141 acgcgagctt cgacgaaaaa ttcctccttg ccgaatgcgc aatgctcgga accgcgccgc
    10201 ggcatgcgca cctgttatgt tccttgaagc tgtcgcgccg gctgctgccc ggcttgcgca
    10261 gctacggctt gtcccggctg tcgtcgtcgc tgggcattgc gttctcgggc gacgcccacc
    10321 gtgccgaggc ggacgccgaa gtcagcgcca aggtcctgct gcacatcggg gaacagctgc
    10381 gccgcagcta tggctttgac gacgtcgggc cggacttgct ggcacgggtg gggagcctca
    10441 gcgccgcgcg tgtgcccgca tttctcgagc gcgaggccgg ccagcgcacc agtgcaccgc
    10501 atgcttgctg aagcatccgg tcgtcatata ccgccggttt atccgcttct gtaacttccg
    10561 attctgatcc cttgatgcgg caactaccgt agaaatacgg tcgccattgc gctttacatt
    10621 tcgcgtccta gtatccagat gccaccggcg tcccaggccg gagccctggc ggcatgaccg
    10681 gcaggccaag aagaacatca ggagacattt cgtgatcaag ccccccctgc gcggcctcac
    10741 tgcggccgct cgctccccgt ttgtccttgc aatcctgctt gccgccagca ccgccgctgc
    10801 ggcgccggta tccctgccgt cctacaacgt cgatgccagc cgcacctccg tgtccggatt
    10861 gtcctccggc ggattcatgg ccgctcagct gcatgtcgcg tactcggcta ccttcaaggc
    10921 cggcgccggc atcgtcgccg gcgggcccta ctactgcgcg cagggcagcc tcacgacagc
    10981 gaccgggcca tgcatggccg cgacgacctg gagcaagccg gcgacgtcga cgctgatctc
    11041 cacgaccaac agctgggcca gccagggcgt gatcgacccg accagcaatc tcaccaattc
    11101 ccgcgtctac ctgttctccg gctcgctcga cagcaccgtg cgcccgctgg tgatggacga
    11161 ggcgaacact tactaccgca attacgtcaa ccacgcgaac gtcttctaca agaaggacat
    11221 cgcggccgag cacgcgatgg tgaccgacta cttcggaaac agctgcagca ccaaggccag
    11281 cccctacatc aacaactgca atttcgatct ggccggcgag atcctgaagt ggatctacgg
    11341 ctcgctcaac gcgaagaaca acggcagcct cggaggcagc ttcatcgagt tcaaccaggc
    11401 ggagttcatc agcaatccga cctcgcgcgg aatggccaac accggctgga tgtacgtgcc
    11461 ggcgaattgc gcggccggac agagctgccg gctgcacgtt gcgctgcatg gctgccagca
    11521 ggacccgacc aagatccagg atcgctacta ccgcaacacc ggctacaaca aatgggccga
    11581 taccaacaac atcatcgtgc tctacccgca gaccgcgccg tcgagcaccg gcaacccgaa
    11641 cggctgctgg gactggtggg gttatgacga tccgaactat gcgaagaaga gcgggcgcca
    11701 gatgctggcc atcaagggca tggtcgaccg catcacgacc ggcggcgcgc cggctccggg
    11761 cgcgcccacc aatctggccg tcaccggcac caccgcgaac tcggtctcgc tgtcctggaa
    11821 tgcggtcagc ggcgcatccg gctaccacgt ctaccgcaac ggcacgcgcg tgacttcctc
    11881 ggcagtggcg gcaacgagct acaccgacag cgggctggca tccgggacaa cctactcgta
    11941 tgcggtgtcg gcgatggtgt ccggcaccga gagtgcgctc tcggccacgg tcagcgccac
    12001 cacgacctcg agcgggcatg catgcacaca aaccacgtca agcaactaca cgcatgtgac
    12061 cgccgggcgc gcctacaaca gtggcggcta tgcactggcc aagggttcca accagaacat
    12121 gggcttgaac aacacgttct acaccaccac gctggcccag accgcgccgg gctactacgt
    12181 gatcggcaac tgcccctgaa cggcacagca cccccgcccg ggggtgcctc aaggagcaag
    12241 gcatgaagac agccagtctc gccgtcgcgc tgtcggccgc ggcgctgtgc gcctggggta
    12301 catgggatca gctcgcaacg cgtcacgaac cggtgcggct gctcgcacgt ccagcgaccg
    12361 ggctggcacc ggtgccggcc gtgggcgcgg cggcgcttcc gcatgttggc gaaggcagcc
    12421 tcgttctgtt tgagcgcgat gcgtcgggcg cgctgcgcat tggccgtcac acgcgcgagc
    12481 ggctcgagga gctcgcccgc tcgctgccgt acgacctgag ccatgcggaa tggcaggcaa
    12541 tcgacgagtt cgcggcagcc ggcctagacg aacctgcggc aagccaggca cgccgcatcg
    12601 tccgtgaaca cctcgccgca cggcaggcgc cgtcgccggg acgagccgcg cctgcaacgg
    12661 cgcatcccga gccgatgacc gagagcctgc tcgcactgcg tccgacgcca ccgccggagc
    12721 aggcagtcat gcgctttccc ggcatgggcg acgaataaag aaaacaacga ggagacacca
    12781 tgaaacgttt caagcacagc accatcgcat cggccgccat gctggccctg atggcctccg
    12841 gcgccgcgtc cgccgtcgtc acgctgccgc gctacaacgt cagcttcgac aatgtgtcgg
    12901 tatccggcat ctcgtccggc ggctacatgg ccgcgcaatt ccatttcgcc tactcgaaaa
    12961 cggtgaagaa aggggccggc gtgatcgctg gcggtccggt ttactgctcg caggggaacg
    13021 tgaacattgc gaccgggccg tgcatggccg acaccgggtc gcgcaatctg ccctatctga
    13081 tctcggtggt gaacacatgg tcgggcaacg gttacatcga cccgacttcg aatcttgcga
    13141 acgcgaaggt ctacctgttc tccggcacca tcgattcgac cgtcaagcag ccggtgatga
    13201 acgacttgcg cacgatgtac ggcaactacg tcagcagcgc caacatcacc tacaagaaca
    13261 acctcgcggc cgaacacgcg atcgtcaccg actactacgg cagtgcctgc agctacaagg
    13321 gcagcccgta catcaacaac tgcaactacg ataccgccgg cgagatcctg aaatggatct
    13381 atggggcgct caatgcgaag aacaccggaa cgctgggcgg cagcttcgtc aatttcaacc
    13441 agcgccacta ctggggcaat gccgatccga ccaaccacgg gatggccaat gacggctggg
    13501 cttacgtccc ggccaactgc gcggccggcc agccctgtcg gctgcatgtc gctttccatg
    13561 gctgccagca gaacgccgcg acgatcggcg acaagttcta ccgcaacacc ggctacaacc
    13621 gctgggccga caccaacaac atcatcgtgc tgtatccgca ggcccacgcg accttcgcga
    13681 atccgaacgg ctgctgggac tggtggggct atgacgatgt gaacttcccc ggcaagagcg
    13741 gcgggcagat ggtcgcgatc aagaccatga tcgaccgcat cgtcagcggc aacgccgctg
    13801 cgccgtacac ctgctcaagc tggtatgcca gcaattattc gcacgtggtc aacggccgcg
    13861 cgtatgcatc caacggcaat gcgtacgcgg tgaattcgaa ccagtacctc gggtattact
    13921 cgacggccgc atacacgaac gtgcgccgca cgtcggccgg gtattacgcg tatggg
//
LOCUS       DATLGF010000518         8898 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_109065, whole genome shotgun sequence.
ACCESSION   DATLGF010000518 DATLGF010000000
VERSION     DATLGF010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 8898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8898
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109065"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VM406_16175"
     CDS             complement(<1..559)
                     /locus_tag="VM406_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVL77551.1"
                     /translation="MSLIENIIRPEIRALSAYHVPDASGFIKLDAMENPYLLPEDMRA
                     ELGQRLADVALNRYPVPSYETLKKKIGARLGVPPGFGLILGNGSDELIALLSIACANP
                     GAKVLAPTPGFVMYSMSARFSGMQFVGVPLKADFSLDMPAMLAAIAEHRPAITYLAYP
                     NNPSGNLYDADDMVRIIEAIGETGIV"
     gene            complement(583..1353)
                     /locus_tag="VM406_16180"
     CDS             complement(583..1353)
                     /locus_tag="VM406_16180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HVL77552.1"
                     /translation="MSDWLDRVFCEDALDGLTRVPDGAVDLLIADPPYGLGKDYGNDS
                     DKLDAEAYLQWTERWVDLALPKLKTNGSLYIFLTWRYSPEIFVMLKKRMTMLNEIIWD
                     RRVPSMGGSTRRFSSVHDTIGFFAKAKDYYFDLDAVRIPYDAQTKKARSRSIFVGAKW
                     LEIGYNPKDVWSVSRLHRVHRERADHPTQKPLEVVERMIKASCPPGGVVLDPFMGSGT
                     TAVAARRCGRHYAGFELNPDYCDLIDVRLAALESPEAA"
     gene            complement(1366..2682)
                     /gene="hisD"
                     /locus_tag="VM406_16185"
     CDS             complement(1366..2682)
                     /gene="hisD"
                     /locus_tag="VM406_16185"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HVL77553.1"
                     /translation="MTITIRKLDSSVPGFTEQLHAVLAFEAAEDEAIDRAAAQILADV
                     KARGDAAVLEYTNRFDKLDARSVAALEIPQAQLRAALEGLAPARRTALQTAAERVRAY
                     HERQKRETGSEGFTYTEADGTVLGQKVTPLDRVGIYVPGGKAAYPSSVLMNAIPAKVA
                     GVQEVIMVVPTPAGVKNELVLAAAAIAGVDRVFTIGGAQAVGALAHGTATIPQVDKIV
                     GPGNAYVAAAKRRVFGVVGIDMIAGPSEILVLCDGTTDPDWVAMDLFSQAEHDELAQS
                     ILVCPDAAYIEAVEASINRQIEAMPRKDVIRTSLTNRGALVKVRDMDEACEIANLIAA
                     EHLEISADNPKQWADKVRHAGAMFLGRFASESLGDYCAGPNHVLPTSRTARFSSPLGV
                     YDFQKRSSVIQVSEAGAQTLGVVAAELAYGEGLQAHAMSAEYRLKR"
     gene            complement(2687..3340)
                     /gene="hisG"
                     /locus_tag="VM406_16190"
     CDS             complement(2687..3340)
                     /gene="hisG"
                     /locus_tag="VM406_16190"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HVL77554.1"
                     /translation="MSQQLTLALSKGRIFEETLPLLQAAGITVTEDPEKSRKLILPTN
                     DPGVRVIIVRASDVPTYVQYGAADFGVAGKDVLMEHGGEGLYQPIDLNIAKCRLSVAV
                     PAGFDYANAVRQGARLRVVTKYVQCAREHFAAKGVHVDLIKLYGSMELGPLVGLSDAI
                     VDLVSTGGTLRANNLVEVEHIMEISSRLIVNQAALKLKRERLQPILEAFEKASAQAA"
     gene            complement(3340..4590)
                     /gene="murA"
                     /locus_tag="VM406_16195"
     CDS             complement(3340..4590)
                     /gene="murA"
                     /locus_tag="VM406_16195"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HVL77555.1"
                     /translation="MDKLLITGGKRLSGEIAISGAKNAALPILCAGLLTADTIELTNV
                     PQLQDVNTMLKLLRQMGLRAEQDGDRMMLSGRAIENCEAPYEMVKTMRASILVLGPLL
                     ARFGEAKVSLPGGCAIGSRPVDQHIKGLQAMGAEIHIEAGYIHAKAKRLKGTRVVTDM
                     ITVTGTENLLMAATLAEGETVLENAAREPEVTDLANLLAAMGAQIDGIGTDRLVVQGV
                     PSLHGASHRVVADRIETGTFLCAVAATGGDVTLTNTRGDLLDVALDKLREAGAILTSG
                     PDWIRVQMAARPKAVSFRTTEYPGFPTDMQAQFMALNCIAEGSSRITETIFENRFMHV
                     QELNRLGAAIDIDGHTAIVQGVDRLVGAPVMATDLRASASLVIAGLAAQGQTLVDRIY
                     HLDRGYDRMEVKLAGVGAEIERVK"
     gene            complement(4590..4826)
                     /locus_tag="VM406_16200"
     CDS             complement(4590..4826)
                     /locus_tag="VM406_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HVL77556.1"
                     /translation="MLPTPEQIKGYIEAGLNCTHVEVEGDGQHFNAVIVSPDFAGKRP
                     IQRHQLVYAALGDRMRAEIHALSMKTLTPEEFLQ"
     gene            complement(4856..5611)
                     /locus_tag="VM406_16205"
     CDS             complement(4856..5611)
                     /locus_tag="VM406_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL77557.1"
                     /translation="MVGFRTLFYKEILRFWKVATQTITAPILTAMLYLLIFGHVLEEH
                     VQVFPGVSYTAFLVPGLVMMSVLQNAFANSSSSLIQSKITGNLVFVLLPPLSHWELYG
                     AYVLASVARGLAVGLGVFLITGFFADLSFVAPWWIAAFAILGAAILGTMGLIAGIWAE
                     KFDQLAAFQNFLIMPATFLSGVFYSIHSLPPFWQTVSHFNPFFYMIDGFRYGFFGHSD
                     VDPARSFVIVAVFFTLLGSLAIALLKSGYKLRH"
     gene            complement(5620..6531)
                     /locus_tag="VM406_16210"
     CDS             complement(5620..6531)
                     /locus_tag="VM406_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL77558.1"
                     /translation="MAALEIVNVEKRYKALQALGGVSLRVEQGEFFGLLGPNGAGKTT
                     LISIVAGLNRADAGSVSIHGFDVVRDYREARQRLGVVPQELVFDPFFTVRETLRMQSG
                     YFGLKKNDDWIDEVMANLDLTNKADTNMRALSGGMKRRVLVAQALVHRPPVIVLDEPT
                     AGVDVELRQTLWKFISRLNREGHTIVLTTHYLEEAQALCNRVAMLKAGRVVALDTTAA
                     LIKRISGSQLIVRLSRGSLPDGLKHLVSHPEELVSGHRYTLRVNEYTEVEPILATLRT
                     AGVVIEDMQLQQADLEDVFIQIMEGEK"
     gene            complement(6627..6890)
                     /locus_tag="VM406_16215"
     CDS             complement(6627..6890)
                     /locus_tag="VM406_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HVL77559.1"
                     /translation="MFRPPLTLTVNNAGLALEAGLRAIEEGQTEIDLGELAAVDSAAV
                     ATLLAWQRAARARGAALRFANVPANLLGLAALYGVDELLRPVP"
     gene            complement(6894..7544)
                     /locus_tag="VM406_16220"
     CDS             complement(6894..7544)
                     /locus_tag="VM406_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL77560.1"
                     /translation="MKSIKQFWTLFALCLGLVAALPASAQTQAETPDALIKRISHQVL
                     DAARTDPQIQAGNHRRVMEVVEEHIFPHVDFQRMTALAVGRFWREATPEQQKRLTEEF
                     RTLLVYTYSGAISQARDQRIEFRPFRADPADNEVEVRSQIVQPRGEPVQLNYRLQRTQ
                     AGWKIYDVSVLGAWLVESYKNTFASEIGKGGIDGLINTLAERNKRLAASAARGSRS"
     gene            complement(7585..8289)
                     /locus_tag="VM406_16225"
     CDS             complement(7585..8289)
                     /locus_tag="VM406_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HVL77561.1"
                     /translation="MMRMLRLCAPLLALLAAGCATTHPDDPFEHYNRVAFGFNRAVDD
                     IALRPAAEVYTRLPSFMQTGVYNFFGNLQDVNTAANNFLQGKWRAGISDVTRVGLNTS
                     LGIAGLFDVASEAGLQKNYEDFGQTLGKWGMESGPYVVLPLLGPSTLRDTLATPVDFQ
                     LDPWYYKRPARYRNAGVVLRTVDQRATLLDASTLLEEAALDRYQFVRDAYLQRRRNQV
                     YDGDPPPLPRAAENDE"
     gene            complement(8286..8756)
                     /gene="mlaD"
                     /locus_tag="VM406_16230"
     CDS             complement(8286..8756)
                     /gene="mlaD"
                     /locus_tag="VM406_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HVL77562.1"
                     /translation="MQRKSIDIWVGLFVLLGAIALFFLAMKAGNMSTFTFQETYAVNA
                     RFDNIGGLKPRAPVKSAGVVVGRVGDIRFDDNTFLAVVTLNLDSRYQFPRDSSAKILT
                     AGLLGEQYIGIEPGGDTENLAQGDRISMTQSAIVLENLIGQFLFSKAAEAGEKQ"
     gene            complement(8801..>8898)
                     /locus_tag="VM406_16235"
     CDS             complement(8801..>8898)
                     /locus_tag="VM406_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL77563.1"
                     /translation="EGVSRATTRTVVIASLVVLWLDFLLTALMFN"
BASE COUNT         1551 a         2845 c         2916 g         1586 t
ORIGIN      
        1 ccacgatgcc ggtctcgccg atggcctcga tgatgcgcac catgtcgtcg gcgtcgtaca
       61 ggttgccgct gggattgttc ggataggcca gataggtgat cgccgggcga tgctcggcga
      121 tcgctgccag catcgccggc atgtcgagcg aaaaatctgc tttcagcggt acgccgacga
      181 actgcattcc cgaaaacctg gccgacatcg agtacatgac gaagcccggt gtcggcgcca
      241 gcaccttggc gcccggattc gcgcaggcga tcgagagcag cgcgatcagt tcgtcggagc
      301 cgttgccgag aatgaggccg aaaccgggcg gcacgccgag cctggccccg atctttttct
      361 tcagcgtctc gtacgagggc accgggtagc ggttgagcgc cacgtcggcc agccgctgcc
      421 ccagttccgc gcgcatgtcc tccggcagca ggtacgggtt ctccatcgcg tcgagcttga
      481 tgaatccgga ggcgtccggc acgtggtacg cggacagcgc gcggatctcg ggacggatga
      541 tgttctcgat cagtgacatg gcaggtctcg ttggtagtag cgtcaggccg cctcggggct
      601 ttcgagggcg gccaggcgca cgtcgatcag gtcgcagtag tccgggttca gctcgaagcc
      661 cgcgtagtgg cggccgcagc gccgcgccgc caccgcggtg gtgccgctgc ccatgaacgg
      721 atcgagcacc acgcccccgg gcgggcagga tgccttgatc atccgctcga caacctccag
      781 cggcttctgc gtgggatgat cggccctctc ccggtgcacg cggtgcaagc gcgacacgct
      841 ccacacatcc ttggggttgt agccgatttc cagccatttc gcgccgacga aaatcgagcg
      901 cgaacgcgcc ttcttggtct gggcgtcgta cggaatgcgc acggcgtcga gatcgaaata
      961 ataatccttc gccttcgcga agaagccgat cgtgtcgtgc accgaggaaa acctgcgggt
     1021 gctgccgccc atcgacggca cccggcgatc ccagatgatt tcgttgagca tcgtcatgcg
     1081 cttcttcagc atcacgaaga tctcgggcga atagcgccag gtcaggaaga tgtacaggct
     1141 gccattggtc ttgagcttgg gcagcgcgag atcgacccag cgctcggtcc attgcaggta
     1201 ggcctcggca tcgagcttgt cggaatcgtt gccgtagtcc ttgcccaggc cgtatggcgg
     1261 atcggcgatc agcaggtcta ccgccccgtc cggcacgcgc gtgaggccat ccagcgcatc
     1321 ctcgcagaac acgcggtcca gccagtcact catcgcggcc gcgcttcatc gcttgagccg
     1381 gtattcggcc gacatcgcat gcgcctgcag gccctcgcca taggcgagtt cggcggcaac
     1441 aacgcccagc gtctgcgcgc cggcttcgct gacctggatg acggacgagc gcttctggaa
     1501 atcgtagacg cccagcggcg acgagaaacg cgcggtgcgc gacgtcggca gcacatggtt
     1561 cgggcccgcg cagtaatcgc cgagcgactc ggacgcgaag cgcccgagga acatcgcgcc
     1621 ggcgtgacgc accttgtcgg cccattgctt cgggttgtcg gcggaaatct cgagatgttc
     1681 ggcggcgatc aggttggcga tctcgcaggc ttcgtccatg tcgcgcacct tgaccagcgc
     1741 gccgcggttg gtcagcgaag tgcggatcac gtccttgcgc ggcatcgcct cgatctggcg
     1801 gttgatgctc gcttcgaccg cctcgatgta ggcggcatcc ggacagacca ggatggattg
     1861 cgcaagctcg tcgtgctcgg cctgggagaa cagatccatg gcgacccagt ccgggtcggt
     1921 cgtgccgtcg cacagcacca ggatctccga gggcccggcg atcatgtcga tgccgaccac
     1981 gccgaacacg cggcgcttgg cagcggcgac ataggcgttg ccgggaccga cgatcttgtc
     2041 gacttgcggg atggtggcgg taccatgggc gagcgcgccg acggcctggg cgccgccgat
     2101 cgtaaacacc cgatcgacgc cggcgatcgc cgcggcggcc agcaccagtt cgttcttcac
     2161 gccggccggc gtcggcacga ccatgatgac ttcctgcacg ccggcgacct tggccgggat
     2221 cgcgttcatc agcaccgacg acggataggc ggccttgccg cccggcacgt agatgccgac
     2281 acgatcgagc ggcgtgactt tctggccaag caccgtgccg tccgcttcgg tatacgtaaa
     2341 gccttcgctg ccagtctcgc gcttctggcg ctcatggtag gcgcgcacgc gctcggccgc
     2401 ggtctgcagc gcggtacgac gggccggggc cagcccttcg agcgccgcgc gcaactgcgc
     2461 ctgcgggatc tccagcgcgg cgacgctgcg cgcatccagc ttgtcgaacc ggttcgtgta
     2521 ttcgagcacg gcggcgtcgc cgcgcgcctt cacgtcggcg aggatctgcg cggccgcgcg
     2581 atcgatcgcc tcgtcctccg ctgcctcgaa cgccagcacc gcgtgcagtt gctccgtaaa
     2641 accgggtacc gaggagtcga gtttgcggat cgtgatcgtc atcgtgtcat gcagcctgcg
     2701 cagaggcttt ctcaaatgct tctagtatcg gctgcaagcg ttcgcgcttg agcttcaagg
     2761 cggcctgatt gacgatcagg cgcgaggaaa tctccatgat gtgctctacc tcgaccaggt
     2821 tgttcgcgcg cagcgtgccg ccggtgctga ccaggtcgac gatggcgtcg gacagcccga
     2881 ccagcggccc gagctccatc gagccgtaca gcttgatgag gtccacgtgc acgcccttgg
     2941 ccgcgaagtg ctcgcgcgcg cactgcacat acttggtcac gacgcgcagg cgcgcgccct
     3001 ggcgcaccgc gttcgcgtag tcgaagccgg ccggcacggc gaccgacagg cggcatttcg
     3061 cgatgttcag gtcgatcggc tggtacagcc cttcaccacc gtgctccatc agcacgtcct
     3121 tgccggccac gccgaaatcc gccgcgccgt actgcacgta ggtcggcaca tctgacgcgc
     3181 gtacgatgat cacacgcacg cccggatcat tggtcggcag aatcagcttg cgcgacttct
     3241 ccgggtcttc ggtgacggtg atgccggccg cctgcagcag cggcagggtc tcctcgaata
     3301 tccgcccctt ggacagcgca agcgtcagtt gctggctcat tacttcaccc gctcgatctc
     3361 ggcaccgacg cccgccagct tgacctccat gcggtcatag ccgcgatcga ggtgatagat
     3421 ccgatcgacc agggtctgtc cctgcgcggc caggccggcg atcaccagcg aggccgacgc
     3481 gcgcaggtcg gtcgccatca ccggcgcgcc gaccagccgg tcgacaccct gcacgatcgc
     3541 ggtgtgaccg tcgatgtcaa tcgcggcgcc gaggcgattg agctcctgca catgcatgaa
     3601 gcggttttcg aaaatcgttt cagtaatgcg gctgctgcct tcggcgatgc agttgagcgc
     3661 catgaattgc gcctgcatgt cggtcgggaa gcccgggtat tcggtggtgc ggaagctgac
     3721 cgccttcgga cgcgcggcca tctgcacgcg gatccagtcg ggtccggagg tgaggatcgc
     3781 gcctgcttcg cgcagcttgt cgagcgcgac atcgagcagg tcgccgcggg tattggtcag
     3841 cgtgacatca ccgccggtgg cggcgaccgc gcacaggaag gtgccggtct cgatgcggtc
     3901 ggcgaccacc ctgtggctcg cgccatgcag gctgggtaca ccctgcacga ccaggcgatc
     3961 ggtgccgatg ccgtcgatct gcgcgcccat tgcggccagc aggttggcca ggtcggtcac
     4021 ctccggctcg cgcgcggcgt tttccagcac cgtctcgccc tcggccagcg tggcggccat
     4081 cagcaggttt tcggtgccgg tgaccgtgat catgtcggtc accacgcggg tgcccttgag
     4141 ccgcttggcc ttcgcgtgga tgtagccggc ttcgatgtgg atctctgcgc ccatcgcctg
     4201 caggcccttg atgtgctggt cgacgggccg cgagccgatc gcgcagccgc cgggcagcga
     4261 caccttggct tcgccgaagc gcgccagcag cggcccgagc acgagaatgg aagcgcgcat
     4321 cgtcttcacc atctcgtacg gcgcttcgca gttctcgatt gcgcgcccgg acagcatcat
     4381 gcggtcgcca tcctgctcgg cgcgcagacc catctgccgc agcagcttca gcatcgtatt
     4441 gacgtcctgc agctgcggaa cgttggtcag ttctatcgta tcggcagtga gcaggcccgc
     4501 gcagaggata ggcagcgcgg cattcttggc gcccgagatg gcgatttcgc cggacaggcg
     4561 tttgccgccg gtgatgagca gtttgtccat tattgcagga actcttccgg ggtcagggtt
     4621 ttcatcgaca gcgcgtgaat ttcggcgcgc atgcgatcac cgagcgccgc atacacgagc
     4681 tggtggcgct ggatcggccg cttgccggcg aaatccggcg agacgatgac ggcattgaaa
     4741 tgctggccgt cgccttcgac ttcaacatgg gtgcagttga ggccggcctc gatatagccc
     4801 ttgatctgct caggggtagg caacatggtg gagctcctgt ctgtgaagcg tgcggtcagt
     4861 ggcgcagctt gtagccgctc ttgagcagcg cgatcgcaag gctgcccagc agcgtgaaga
     4921 atacggcgac gatgacgaag ctacgggcgg gatcgacgtc cgaatgaccg aagaagccgt
     4981 agcggaagcc gtcaatcata taaaaaaacg gattgaagtg cgacaccgtc tgccagaacg
     5041 gcggcaatga gtggatcgaa taaaacacac ccgacaggaa ggtggccggc atgatcagga
     5101 agttctggaa cgcagcgagc tggtcgaact tctccgccca gatgccggcg atcaggccca
     5161 tggtgccgag gatggcggcg ccgaggatcg cgaatgcggc gatccaccat ggcgccacga
     5221 aggagagatc cgcgaagaaa ccggtgatca ggaacacgcc caggccaacg gcgagaccgc
     5281 gcgccaccga ggccagcaca taggcgccat acagctccca atgggacagc ggcggcagca
     5341 gcacgaagac caggttgccg gtgatcttcg actggatcag cgacgacgaa ctgttggcga
     5401 aggcgttctg cagcacgctc atcatcacca gccccggcac gaggaaagcg gtatagctga
     5461 cgccggggaa gacctgcaca tgttcttcca gcacatggcc gaagatcagc aggtacagca
     5521 tcgccgtcag gatcggtgcg gtgatcgtct gcgtggcaac cttccagaag cgcaggatct
     5581 ccttgtagaa cagcgtgcgg aagccgacca tgccaccggt catttttcac cctccatgat
     5641 ctggatgaac acgtcctcca ggtcggcctg ctgcaactgc atgtcttcga tgacgacgcc
     5701 ggcggtgcgc agcgtggcga gtatcggttc gacttcggtg tactcgttca cccgcagggt
     5761 gtaacggtgg ccggacacca gttcctcggg atgcgagacc aggtgcttca ggccgtccgg
     5821 caggctgccg cgcgacaggc gcacgatgag ctgggaaccg gagatgcgct tgatcagtgc
     5881 cgcagtcgta tccagggcaa ccacccggcc cgccttcaac atcgccaccc ggttgcacag
     5941 ggcctgcgct tcttccaggt agtgcgtggt cagcacgatg gtgtggcctt cgcggttcag
     6001 gcgcgagatg aatttccaca gcgtctggcg cagttccacg tcgacacccg cggttggctc
     6061 gtcgagcacg atcaccggcg gtcggtgcac cagcgcctgg gcgacgagga cgcgtcgctt
     6121 catgccgccg gacagcgcgc gcatgttggt gtcggccttg ttggtcaggt cgagattggc
     6181 catcacctcg tcgatccagt cgtcgttctt cttcagtccg aaataaccgg actgcatgcg
     6241 cagcgtctcg cgcaccgtga agaacggatc gaacaccagt tcctgcggca cgacgcccag
     6301 ccgctggcgc gcttcgcggt agtcgcgcac cacgtcgaag ccgtggatgc tgacactgcc
     6361 ggcatcagcg cggttgagcc cggcgacgat agagatcagc gtcgtcttcc ccgcgccgtt
     6421 ggggccgagc agaccgaaga actccccctg ctcgacgcgc agggacacgc caccaagggc
     6481 ttgcagcgcc ttgtagcgtt tttcgacgtt gacgatctct agggcagcca tgcggcgcaa
     6541 atttcgggaa gggaagccgg cgcaccgagg tgtgcgccct tggggcgaac cggagattat
     6601 aggagatttg ccggcagtca gccgactcag gggaccggcc gcaggagttc atccacgccg
     6661 tacagagcgg ctaggccgag caggttggcg ggcacgttgg caaacctcaa cgcggcgccg
     6721 cgggcgcgcg cagcccgctg ccaggcgagc aaggttgcga ctgctgcgga atcgacggcg
     6781 gccagctcac cgaggtcgat ctcggtctgg ccctcttcga tcgcgcgcag cccggcttca
     6841 agtgccaggc ccgcattgtt caccgtcagc gtaagcggag gacggaacag cattcaggaa
     6901 cgcgacccgc gcgccgcact ggctgccagg cgcttgttgc gctcggccag cgtattgatc
     6961 aggccgtcga tgccgccctt gccgatctcg gaggcgaacg tgttcttgta actctcgacc
     7021 agccaggcgc cgagcacgct cacgtcgtag attttccagc ccgcctgggt tcgttgcagc
     7081 cggtagttca gctggaccgg ctcgccgcgc ggctgcacga tctgggaacg cacttccacc
     7141 tcgttgtcag ccggatcggc gcggaacggg cggaactcga tgcgctggtc acgcgcctgg
     7201 ctgatcgcgc cggagtaggt gtagaccagc agcgtgcgga actcttcggt cagacgcttc
     7261 tgctgctcgg gcgtcgcctc gcgccagaag cggccgaccg ccagggcggt catgcgctgg
     7321 aaatcgacgt gcgggaagat gtgctcctcg accacttcca tgacgcgccg gtggttgccg
     7381 gcctggatct gcgggtcagt gcgcgcggcg tcgagcacct ggtggctgat gcgcttgatc
     7441 agcgcgtcgg gtgtctccgc ctgcgtttgg gcgctggccg gcaaggcggc caccaggccc
     7501 aggcagagtg caaagagcgt ccagaattgc ttgatggatt tcataattct ctcggattga
     7561 aacgggttgt gcagcgccgg cgtgtcattc gtcattctcg gcggcgcgag gcagcggcgg
     7621 cggatcgccg tcatagacct ggttgcggcg gcgctgcaga taggcatcgc ggacgaactg
     7681 gtaacgatcg agcgcggcct cctccagcag ggtcgacgca tccagcaggg tagcacgctg
     7741 gtcgaccgtg cgcaggacga cgccggcgtt gcggtagcgc gccggccgct tgtagtacca
     7801 cgggtccagc tggaaatcga ccggcgtggc gagcgtgtcg cgcagcgtcg acggcccgag
     7861 caagggaagc accacgtacg gccccgattc catgccccac ttgcccagcg tctggccgaa
     7921 atcctcgtaa ttcttttgca agccggcttc gctggcgaca tcgaacaacc cggcgatgcc
     7981 tagcgaggta ttcaggccga cgcgcgtcac atccgatatg cccgcacgcc attttccctg
     8041 gaggaaattg ttcgcggcgg tgtttacgtc ttggaggttg ccgaagaagt tgtatacacc
     8101 ggtctgcatg aaggacggca ggcgggtgta cacctcggcg gcgggccgca aagcgatatc
     8161 gtcgacggcg cggttgaacc cgaacgcgac ccggttgtag tgctcgaacg ggtcgtccgg
     8221 gtgggtcgtg gcgcagccgg cggcgagcaa ggccaatagc ggcgcgcaca ggcgcagcat
     8281 gcgcatcatt gcttttcccc ggcttcggcg gccttgctga acaggaactg gccgatcagg
     8341 ttttcaagca cgatggccga ctgggtcatc gagatcctgt cgccctgcgc aagattttcc
     8401 gtatccccgc ccggctcgat gccgatgtac tgttcgccaa gcagtccggc ggtgagaatc
     8461 ttggcggaac tgtccctcgg aaactggtag cgcgaatcca gattgagcgt aaccaccgcg
     8521 agaaaggtgt tgtcatcgaa gcggatgtcg cccacgcgcc ccaccactac ccctgcgctc
     8581 ttgaccggcg cgcgcggctt caggccgccg atgttgtcga agcgggcatt gaccgcgtat
     8641 gtttcctgga aggtgaacgt actcatgttc ccggccttca tcgcgaggaa aaacagggcg
     8701 atcgcgccca gcagcacgaa cagtcccacc cagatgtcta tcgatttgcg ttgcatgatt
     8761 ctcttctacc agaggcttgc ggcacctgaa tgggccgccg tcaattgaac atcagtgcag
     8821 tcagcaggaa atccagccac agcacgacca gcgaagcgat gacaacggtt cgcgtggttg
     8881 cgcgcgacac accttccg
//
LOCUS       DATLGF010000519         4106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_236809, whole genome shotgun sequence.
ACCESSION   DATLGF010000519 DATLGF010000000
VERSION     DATLGF010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236809"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..762)
                     /locus_tag="VM406_16240"
     CDS             complement(67..762)
                     /locus_tag="VM406_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HVL77564.1"
                     /translation="MRSLAPLARFVAALSFGFMALGVSAAPDNPQSGVDYRTLDKPQP
                     TESGNRVEVIEFFWYSCPHCNSLEPSLNDWVKKQGDTIAFRRVPIAFRESMMPQQKLY
                     YTLEAMGNLETMHPKVFHTIHVQRRPLDSETAIVNWVVSQGIDKQKFLDVFNSFGVQS
                     KVRRAQQLQQAYQVDGVPMLAVDGRYVTSPAIISSSIGNRPEPVLYAATFQVMDALVA
                     RAAKDKRAAAPAK"
     gene            complement(831..1433)
                     /locus_tag="VM406_16245"
     CDS             complement(831..1433)
                     /locus_tag="VM406_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HVL77565.1"
                     /translation="MKQNRQRGGTLLGIVIGLLLGLGIALAVAMVVTKTPIPFTDKSG
                     RQKAAEPTARQSADPNQPMYRRRGAAPETAEVAPPVNTAAPLPAAQQEVRVAPAQPIE
                     PGQAVIIEQSATERAAAQEEKWQYYLQAGAFREMHDAESMRAKLALLGVEARVTERHA
                     DTGLLFRVRAGPFDQLEAMNRVRTKLTDNGIDAAVVRISR"
     gene            complement(1480..3216)
                     /gene="argS"
                     /locus_tag="VM406_16250"
     CDS             complement(1480..3216)
                     /gene="argS"
                     /locus_tag="VM406_16250"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HVL77566.1"
                     /translation="MLAFQKQQILDLFQAALAPVIAGTSLQPSLSLERPRDPAHGDIA
                     CNIAMQIAKPLKKNPREVAQQIVSAVLDNPARADLIDSAEIAGPGFINLRVSAVAKQA
                     VVKVVQQQGAQFGRGNAGAGRKVMVEFVSANPTGPLHVGHGRQGALGDALSALFEAQG
                     YAVTREFYYNDAGAQIGNLAYSVQARSRGHKPGDAHWPESAYNGDYIADIAADFLAKK
                     TVAASDGAPITASGDPNDLESIRMFAVAYLRREQDLDLQAFGVKFDNYYLESSLYHDG
                     KVEAAVEALVKAGKTYELDGALWLKTTDYGDDKDRVMKKGDGSYTYFVPDVAYHVTKW
                     QRGYQKVINVQGSDHHGTIARVRAGLQALGLGIPQGYPDYVLHKMVTVMKNGEEVKIS
                     KRAGSYVTVRDLIEWSAGDAPVNEQGERDITRGRDAVRFFLISRKADTEFTFDVDLAL
                     SQSDENPVYYVQYAHARICSVFAQWNGDEQALNDVDLSPLTSPREAALLARLAEYPEM
                     LQKALDELGPHQVAFYLRDLAGELHSYYNAERVLVDDESLRMARLALLQATRQVLRNG
                     LALIGVSAPARM"
     gene            complement(3326..>4106)
                     /locus_tag="VM406_16255"
     CDS             complement(3326..>4106)
                     /locus_tag="VM406_16255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL77567.1"
                     /translation="VLVDAARFEASLLNLLVNARDAMPAGGTVTISTANVDLGPGEVG
                     RLAPGSYVRVTVKDSGTGMPPEVLDRAFEPFFTTKEVGKGTGLGLSQVYGFIAQSGGE
                     VQVRSKEGKGTTVSMFLPANTGGTASEGKGGTATAAAAQKDVVLVVDDDPDLLDIGAE
                     LFRNLGYEVLTARDAAQATSVLRERSDIGILFSDVVMPGDKSGIELARAARSQRPDLR
                     VILASGYPLQALKAAHGELNEFTLMAKPYRLGDLVNTLRAA"
BASE COUNT          659 a         1350 c         1340 g          757 t
ORIGIN      
        1 aaaatccgcc accctcgcgg atggcggact tcattatcgc cgaatcgccg tggctgccgt
       61 gccggcttac ttcgcgggcg cggcggcgcg cttgtccttc gcggcgcgcg cgaccagcgc
      121 gtccatcacc tggaaggtcg ccgcgtacag gaccggctcc ggacggttgc cgatgctgct
      181 gctgatgatc gccggtgacg tcacatagcg gccgtcgacc gccagcatcg gcacgccatc
      241 gacctggtag gcctgttgca gctgctgcgc acggcgcacc ttgctctgca cgccgaagga
      301 attgaacaca tcgaggaact tctgcttgtc gatgccctgc gacacgaccc agttcacgat
      361 cgcggtttcg ctgtcgagcg ggcggcgctg cacatggatc gtatggaaca ccttcggatg
      421 catggtttcc aggttgccca tcgcctcaag cgtgtagtac agcttttgct gcggcatcat
      481 cgattcgcgg aacgcgatcg gcacgcgcct gaacgcgatg gtgtcgccct gcttcttcac
      541 ccagtcgttc agcgacggtt ccagcgagtt gcagtgcggg caggaatacc agaagaattc
      601 gatgacttcg acgcgattgc cggattcggt cggctgcggc ttgtcgagcg tgcggtagtc
      661 gacgccgctt tgcggattgt cgggggcggc cgagacgccc agggccatga agccgaaact
      721 gagggcggcg acgaaccggg cgaggggagc gagtgaacgc atatgcttcc tttggttgcc
      781 tgaatggtta tcgaaagtga gtgacgggca gatcgatcgg atcgatcgaa tcagcgcgag
      841 atgcggacca cggcggcatc gatgccgtta tcggtcagct tcgtgcgcac gcgattcatc
      901 gcctcgagct ggtcgaacgg gccggcgcgc acgcgaaaca gcaggccggt gtccgcatgg
      961 cgctcggtca cgcgcgcctc cacgccgagc agggccagct tcgcccgcat gctctccgcg
     1021 tcgtgcatct cgcggaacgc gcccgcctgc agatagtact gccacttttc ctcctgcgct
     1081 gccgcgcgtt cggtggccga ctgctcgatg atcaccgcct ggccgggctc aatcggctgc
     1141 gctggcgcaa cacgcacttc ctgttgcgcc gccggcaacg gggctgcggt attgaccggg
     1201 ggcgcaactt ccgccgtttc cggcgcggcg ccgcgccggc ggtacatcgg ctgattcggg
     1261 tcggcactct gacgtgcagt cggttccgcc gccttctggc ggcccgactt gtcggtaaac
     1321 ggaatcggcg tcttcgtgac caccatcgcc accgccagcg cgatgccaag accgagcaac
     1381 agtccgatca caataccgag cagcgtaccg ccgcgctgcc tgttctgctt cattgaatta
     1441 cctcttggtt acagctgata catgcgccta catcgcgcat cacatccgcg ccggcgccga
     1501 cacgccgatc agcgcgagcc cgttgcgcag aacctggcgc gtggcctgca gcaatgccag
     1561 gcgcgccatc cgcaacgatt cgtcgtcgac cagcacgcgt tcggcgttgt agtagctgtg
     1621 cagctcgccg gccagatcgc gcagatagaa cgctacctga tgcgggccga gttcgtcgag
     1681 cgccttctgt aacatttccg gatattccgc gagcctggcc agcagcgccg cctcccgcgg
     1741 tgaagtcaac ggagacagat cgacatcgtt caatgcctgt tcgtcaccat tccactgcgc
     1801 aaataccgag cagatgcgcg cgtgcgcgta ctgcacgtag tacaccgggt tctcgtcgga
     1861 ctgcgacagt gccagatcga catcgaaggt gaactcggtg tcggccttgc gcgagatgag
     1921 gaagaagcgc accgcatcgc gcccgcgcgt gatgtcgcgc tcgccctgct cgttgaccgg
     1981 agcgtccccc gccgaccatt cgatcaggtc gcgcacggtg acgtaggagc cggcgcgctt
     2041 ggagattttc acctcctcgc cgttcttcat cacggtcacc atcttgtgca gcacgtaatc
     2101 cggatacccc tgcggaatgc cgaggcccag cgcctgcaga ccggcgcgca cgcgcgcgat
     2161 ggtgccgtgg tggtcgctgc cctgcacgtt gatcaccttc tgatagccgc gctgccactt
     2221 ggtcacgtga taggcgacat ccggcacgaa atacgtgtag gagccgtcgc ccttcttcat
     2281 cacgcggtcc ttgtcgtcgc cgtagtcggt ggttttcagc cacagcgcgc cgtcgagctc
     2341 gtaggtcttg ccggccttga ccagcgcctc gaccgccgcc tcgaccttgc catcgtggta
     2401 cagcgaggat tcgagatagt agttgtcgaa cttcacgccg aaggcctgca ggtccaggtc
     2461 ctgctcgcgg cgcaggtagg cgaccgcgaa catgcggatc gactccaggt cgttcgggtc
     2521 gccgctggcg gtgatcggcg cgccgtcgct ggcggcgacc gtcttcttgg cgaggaagtc
     2581 ggcggcaatg tcggcgatgt agtcgccgtt gtaggcggac tcgggccagt gcgcgtcgcc
     2641 cggcttgtgg ccgcgcgagc gcgcctgtac cgagtaagcg aggttgccga tctgcgcgcc
     2701 ggcgtcgttg tagtagaact cgcgcgtgac cgcgtagccc tgggcctcga acagggccga
     2761 cagcgcatcg ccgagcgcgc cctggcggcc gtggcccaca tgcagcgggc cggtcgggtt
     2821 ggccgacacg aattcgacca tcaccttcct tccggcgccg gcattgccgc ggccgaactg
     2881 cgcgccctgc tgctgcacga ccttgacgac cgcctgcttc gccacggcgg acacacgcag
     2941 gttgatgaat cccggtccgg cgatctcggc gctgtcgatc aggtcagcgc gcgccgggtt
     3001 gtcgagcacg gcactgacga tctgctgcgc gacttcgcgc ggattcttct tcagcggctt
     3061 ggcgatctgc atcgcgatgt tgcaggcgat gtcgccgtgc gccggatcac gcggccgttc
     3121 gagcgaaaga ctgggttgca ggctggtgcc ggcgatcacc ggcgcaagcg cggcctggaa
     3181 caggtcgaga atctgttgtt tctggaatgc gagcatggaa acgtcgatgc cggccgcccg
     3241 ggccggctgc tggatggagg atgggcagca ttataccggg cgggtacgtc gcttcgcggc
     3301 aagtcggcgc ccggcggcta ggcggctagg cggcgcgcag cgtgttgacc aggtcgccca
     3361 gccgataggg ttttgccatc agcgtgaatt cgttcagctc gccgtgcgcg gccttcagtg
     3421 cctgcagcgg atagccggac gcgaggatca cccgcaagtc cggcctctgc gaacgggccg
     3481 cgcgcgcgag ttcgatgccg ctcttgtcgc ccggcatcac gacgtcggag aacagaatgc
     3541 cgatatcgct gcgctcgcgc agtaccgacg tcgcctgtgc cgcgtcgcgc gcggtcagca
     3601 cttcatagcc gagattgcga aacagctccg cgccgatgtc cagcaggtca gggtcgtcat
     3661 cgacgacgag tacgacgtcc ttctgcgcgg cggcggccgt ggccgtgccg cccttgcctt
     3721 cactggctgt cccgccggtg ttggcgggaa ggaacatgga aaccgtggtt cccttgccct
     3781 ccttgctcct gacctgcact tcgcccccgg actgcgcaat gaagccgtac acctggctca
     3841 agccgagtcc ggttcccttg ccgacctcct tggtggtgaa gaatggttcg aaggcccggt
     3901 ccaacacctc cggaggcata ccggtgccgg aatccttcac ggtgacccgg acatacgagc
     3961 cgggagccag ccggccgact tcgcccggac cgaggtcgac attggcggtg gagatcgtca
     4021 cagtgccgcc ggcgggcatc gcatcgcgtg cattgaccag aaggttgagc agggaggcct
     4081 cgaaacgggc cgcgtccacc aggacc
//
LOCUS       DATLGF010000520         2505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_221296, whole genome shotgun sequence.
ACCESSION   DATLGF010000520 DATLGF010000000
VERSION     DATLGF010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221296"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /gene="era"
                     /locus_tag="VM406_16260"
     CDS             <1..571
                     /gene="era"
                     /locus_tag="VM406_16260"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HVL77568.1"
                     /translation="VLDLLPKNVPVVLVINKSDRIKDKAVMMPFAQQVAAAHPFAAIV
                     PVSAKQRFQLAELEREIEKLLPVNPPMFGEDDITDRSERFLAAEIVREKVFRFVGDEL
                     PYTSTVVIEKFEEEGRLRRVFAAILVERDSHKAMVIGNKGAKLKEISTQARLDMEKLF
                     DGPVYLEIWVKVKSGWADNEAGLRAYGYE"
     gene            613..1419
                     /gene="recO"
                     /locus_tag="VM406_16265"
     CDS             613..1419
                     /gene="recO"
                     /locus_tag="VM406_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HVL77569.1"
                     /translation="MSASASQGTFRSENGSPAAPSAAPRPSTRNRSDVRVADQPAFVL
                     HSYPYKETSLIVDLFSRDYGRVALVAKGAKRPHSKLRGVLQTFQPLSVGWSGKGEVRT
                     LTSADWIGGLLPLEKSALLCGFYLNELLVKLLAREDPHPALFDHYVATLNQLAHGEPA
                     PIVLRRFERALLKETGVGGDFTVCRSSGEPVHPEAIYVVDPEQGPRPARGSDNWPRVA
                     GKTLLDMEAEDYGDPATQAQSKQLMRFLLAHHLAGIPLNTRQILIDLMQL"
     gene            1436..2203
                     /gene="pdxJ"
                     /locus_tag="VM406_16270"
     CDS             1436..2203
                     /gene="pdxJ"
                     /locus_tag="VM406_16270"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HVL77570.1"
                     /translation="MSLLNPSSPVIDLGVNIDHVATVRNARGAPYPDPVAAALLAEEA
                     GADCITLHLREDRRHIRDADVEALRPQLRTRMNLEAAVTPEMLDFACRIKPEDVCLVP
                     ERREELTTEGGLDVVGRFADVKAACSQLQRSGIRVSLFVDPDPVQIRAAAEAGAPVIE
                     IHTGRYAHAEGEAAVQAELERVTSAVMEGRRYGMKVNAGHGLHYTNVQAIAAIAEIEE
                     LNIGHAIVAHALFVGWQNAIKEMKAIMVAARLGLHKK"
     gene            2200..>2505
                     /gene="acpS"
                     /locus_tag="VM406_16275"
     CDS             2200..>2505
                     /gene="acpS"
                     /locus_tag="VM406_16275"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HVL77571.1"
                     /translation="MIYGIGTDIVQIARIEAALARHGERFAERILGAEELIKFRRRRE
                     KVEARGVRFLATRFAAKEAFSKAIGLGMNMPMTWRAMQTLNAPSGKPVAVTSGVLKEF
                     "
BASE COUNT          468 a          765 c          823 g          449 t
ORIGIN      
        1 ggtgctcgac cttctcccga agaatgttcc ggtggtgctc gtcatcaaca agtccgaccg
       61 gatcaaggac aaggcggtga tgatgccgtt tgcccagcag gtggcggccg cgcatccgtt
      121 tgccgccatc gtcccggtgt ccgcgaagca acgcttccag cttgcagagc tcgagcgcga
      181 gatcgaaaag ctcctgccgg tcaacccgcc gatgttcggc gaggacgaca ttaccgatcg
      241 cagcgaacgc tttctggcgg cggaaatcgt acgtgaaaag gtgtttcggt tcgtcggcga
      301 cgaattgccc tatacaagta ccgtcgtgat cgagaaattc gaggaagagg ggcgtctgcg
      361 ccgcgtattc gcggcgatcc tggtcgaacg cgacagtcac aaggccatgg tcatcggcaa
      421 caagggggcg aagctgaagg aaatttcgac gcaggcgcgc ctggacatgg agaagctgtt
      481 cgacggaccg gtctatctcg agatctgggt caaggtcaag tccggttggg ccgacaatga
      541 agccggattg cgcgcctacg gctacgagta acggccgcat tcgcgcctcc cacgtgttca
      601 ttcgatcgcg ccatgtctgc gtccgcatcc cagggcactt tccggtctga gaacgggtca
      661 cccgctgcac cgtcggcggc gccgcgaccg tccacgcgca accgaagcga tgtgcgagtc
      721 gccgatcaac cagccttcgt gctccacagc tatccgtaca aggaaaccag cctgattgtc
      781 gacctgttct cgcgcgacta cgggcgggtc gcgctcgtgg cgaagggggc taagcgtccc
      841 cactcgaagc tgcgcggcgt gctgcagacc ttccagccct tgtccgtagg ctggagcggc
      901 aagggggaag tgcgcacatt gacgtcggcc gactggatcg gcggcctgtt gccgctggag
      961 aaatcggcgc tgctgtgcgg tttttacctg aacgaactgc tcgtcaagct gctcgcgcgc
     1021 gaggatccgc acccagcctt gttcgaccac tatgtcgcca cattgaatca gctcgcgcat
     1081 ggcgaaccgg cgcctatcgt cttgcgtcgt ttcgagcggg cactgctgaa ggaaaccggc
     1141 gtcggcggcg atttcacggt gtgccgcagc agtggcgagc cggtgcatcc cgaggcaatc
     1201 tacgtggtcg atcccgagca gggcccgcgt ccggcgaggg ggagcgacaa ctggccgcgg
     1261 gttgccggca agacgctgct ggacatggaa gcggaggatt acggcgatcc cgccacccag
     1321 gctcagagca agcagctgat gcgttttctg cttgcgcatc atctggccgg cattcccctg
     1381 aatacgcgcc agattctgat agatttgatg cagttataaa ccttcctccc gcgccatgag
     1441 ccttctgaat ccttcctccc cggtcatcga tctgggtgtc aatatcgatc acgtcgcgac
     1501 agtgcgcaac gcgcgcggcg ctccctatcc cgatccggtg gccgccgcct tgctggcgga
     1561 ggaggccggt gccgattgca tcaccttgca tttgcgcgaa gaccgccgtc atatccgcga
     1621 tgcggacgtg gaggcgctgc gcccgcagct gcgcacgcgc atgaacctgg aggcggcggt
     1681 cacgccggaa atgctggact tcgcctgccg catcaagccc gaggacgtct gcctggtgcc
     1741 ggaacggcgt gaagagctga ccaccgaagg cgggctcgac gtggtcgggc gtttcgccga
     1801 cgtgaaggcg gcctgcagcc agctgcagcg ctccggcatc cgggtgagcc tgttcgtcga
     1861 ccccgatccg gttcagatcc gggccgcggc tgaggctggc gcgccggtca tcgaaatcca
     1921 caccggacgc tatgcgcatg ccgaaggaga ggcggcggtc caagccgaac tcgaacgcgt
     1981 caccagcgcg gtgatggagg ggcggcgcta cgggatgaag gtcaatgccg gccatggcct
     2041 gcattacacc aatgtccagg cgatcgccgc gattgccgag atcgaggagc tcaatatcgg
     2101 tcacgcgatc gtggcgcatg cgctgttcgt cggctggcag aacgcgatca aggaaatgaa
     2161 ggcgatcatg gtcgcggccc gtttgggact gcacaagaaa tgatttacgg gatcggtacc
     2221 gacatcgtgc agatcgcgcg catcgaggca gcgcttgccc gccatggcga gaggtttgcc
     2281 gagcgcatcc tgggggcgga ggaactgatc aaattccgtc ggcggcgcga gaaagtggaa
     2341 gcgcgcggcg tgcgcttcct cgccacgcgt tttgccgcaa aggaagcttt ctcgaaggcg
     2401 attggcctcg ggatgaacat gccgatgacc tggcgcgcga tgcagacgct caatgcgccc
     2461 agcggaaagc cggttgcggt gaccagcggc gtcctcaagg aattc
//
LOCUS       DATLGF010000521         6033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_124477, whole genome shotgun sequence.
ACCESSION   DATLGF010000521 DATLGF010000000
VERSION     DATLGF010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124477"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VM406_16280"
     CDS             <1..189
                     /locus_tag="VM406_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HVL77572.1"
                     /translation="VLKEMLEGLGATVTEEMAPFEPESGAYGGGHHHHHGDHDHHHHH
                     PLAPIPLRQKIHRPSDKS"
     gene            192..872
                     /locus_tag="VM406_16285"
     CDS             192..872
                     /locus_tag="VM406_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HVL77573.1"
                     /translation="MQPAALFHLLQLASPSLPIGAYSYSQGLEAALEQGLVKDQASAR
                     AWIADALHEVVARFEAPILWRLMQGFAQRDAAVVTLWTERFLAARDTAEFRAETVQMG
                     FSLGKLVQELQLGDEAMQAILQAQPEVPLPTALAYAMVALDVPHEAALSGALFSWAEN
                     QVLVCVKSVPLGQVAGQRMLLSLQPDLERAAATAQLLEDDALSNWSPGLSLLSMQHEV
                     QYSRLYRS"
     gene            891..1511
                     /gene="ureG"
                     /locus_tag="VM406_16290"
     CDS             891..1511
                     /gene="ureG"
                     /locus_tag="VM406_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HVL77574.1"
                     /translation="MKNPLRVGIGGPVGSGKTALCEMLCKSMRDHYDMAVITNDIYTK
                     EDMEILLRAEALPAERLMGVETGGCPHTAIREDASINLEAIARMSADFPDLDLILVES
                     GGDNLAATFSPELSDLTIYVIDVAGGEKIPRKGGPGITRSDLLIINKTDLAPYVGANL
                     DVMASDAKKMRGDKPFVFTNLRKGEGVDMVIAFIRREGLLDDVKKR"
     gene            complement(1609..2667)
                     /locus_tag="VM406_16295"
     CDS             complement(1609..2667)
                     /locus_tag="VM406_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HVL77575.1"
                     /translation="MNNPAFQQKSFFALLITVSIAFIWVLLPFYGAVFWGTVLAILFT
                     PFYRQLLAGMRGRRNLAALTTLFLVLVIVIFPMSVITMSLVKEVNLVVQMIRTGQLDF
                     GAYFLQIINALPPWAVEILDRYELGTIYAIRERLSEAALQGSQLFASQALNIGQNTFH
                     FVVSFAIMLYLLFFLLRDGATLSARIRHAIPLSEEHKRLLISKFTTVIRATVKGNIVV
                     AVTQGALGGLIFWILGIQGALLWAVLMAFLSLLPAVGASLIWGPVAIYFLVTGAVWQG
                     VVLIAFGVFVIGLVDNILRPILVGKDTKLPDYVVLISTLGGMALFGLNGFVIGPLIAA
                     LFIAAWDLLASTREARAE"
     gene            complement(2875..4002)
                     /locus_tag="VM406_16300"
     CDS             complement(2875..4002)
                     /locus_tag="VM406_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HVL77576.1"
                     /translation="MSPWSAAQPGAPAAQIDTPALLLDLDAFDRNLARMQAALAGSGT
                     RLRPHAKSHKCPEIARRQIAAGAVGICCQKLAEAAVFVNAGIEDVLITNQVVGPAKLQ
                     RLAELATRARIGVLVDHARQAQDLSAAAHARGARVDVYVEVDVGAGRCGVRADQAGAL
                     AAQIVALPALRFAGLHCYHGSAQHLRRPEERQAAIAAAAAHARAARDAVEAAGIPVGI
                     ITGAGTGSFVHERDSGVYNELQPGSYIFMDRDYADNTAGPDDIRFEHALFVKTTVQSR
                     PVPGRAVVDAGLKASSVDSGMPAPWQQPDVGYLKASDEHGVLGGDDGALPAMGESLLL
                     VPGHCDPTVNLYDELVCVRGLASGAGTVEAVWKIEARGALL"
     gene            4204..4965
                     /locus_tag="VM406_16305"
     CDS             4204..4965
                     /locus_tag="VM406_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HVL77577.1"
                     /translation="MNASVLLTNLVAALLLPPLNAVLLVLLGLMLRRRLPRVGVALVL
                     LALAALTVFSTFAGARWLHGPLERTHPPLQAPHLHDAQAIVLLGGGRIEELAEYGGVD
                     IPFPTAFLRMHYAARLHRQTGLPVLASGGVPDGAGESEAAMMARMLHEDYAVPVQWLE
                     ERSNNTAQNAKYTAAILLPQGLRRILLVTDAMHMARATAVFERAGFEVVPAPTARLSE
                     GPLHALDLVPNGEGLRRSHYALRERIGQLWYRLRH"
     gene            5720..>6033
                     /locus_tag="VM406_16310"
     CDS             5720..>6033
                     /locus_tag="VM406_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglutaminylglutamine amidotransferase"
                     /protein_id="HVL77578.1"
                     /translation="MRAVLDMTDAQKRRGPNGEGIFSLGARCFGHRRLSIMDLSQRAH
                     QPFVDNTLGMGIVFNGAIYNHHELRAELREKGYQFASNGDTEVIIKAWHAWGPKALDR
                     FYG"
BASE COUNT         1087 a         1967 c         1982 g          997 t
ORIGIN      
        1 gtactcaagg aaatgctgga aggcctgggc gcaaccgtga ccgaggagat ggccccgttc
       61 gagcccgagt ccggcgccta tggcggcggc catcaccacc accacggtga ccacgatcat
      121 caccaccatc atccgctggc gccaatcccg ctgcgccaga agatccaccg tccgtccgac
      181 aagtcatgag catgcagccg gccgcgctgt tccacctgct gcaactggcc agtccgtcgc
      241 tgccgatcgg cgcctacagc tactcgcaag gactggaggc ggcgctcgag caaggcttgg
      301 tgaaggacca ggccagcgcg cgcgcatgga ttgcggatgc cttgcatgaa gtggtggcgc
      361 gtttcgaagc accgattctg tggcggctga tgcagggctt cgcccagcgc gacgcggcgg
      421 tcgtcacgct gtggaccgag cgcttcctcg ccgcgcgcga caccgccgag tttcgcgccg
      481 agaccgtgca gatgggtttc tcgctgggca agctggtgca ggaactgcaa ctgggcgacg
      541 aagccatgca agccatcctg caggcccagc cggaagtccc gctgccgacc gcgttggcct
      601 atgcgatggt ggcgctggac gtgccacacg aggcggcgct gagcggcgcg ctgttctcgt
      661 gggcggagaa ccaggtactg gtctgcgtga aatccgtgcc gctcgggcag gtggccgggc
      721 agcgcatgct gctgtccttg cagccggacc tggagcgcgc cgccgccact gcgcagttgc
      781 tggaggacga cgccttgtcc aactggtcac ccggcctgtc gctgctctca atgcagcacg
      841 aagtgcaata cagccgtctt taccgttcct gaaagattga aggaagaacc atgaaaaatc
      901 ccctgcgcgt cggcatcggc ggcccggtcg gctccggcaa gaccgccttg tgcgagatgc
      961 tgtgcaagtc catgcgcgac cactacgaca tggccgtcat caccaacgac atctacacga
     1021 aggaggacat ggagatcctg ctgcgcgccg aagccctgcc ggccgagcgg ctgatggggg
     1081 tcgagaccgg tggctgtccg cacacggcca tccgcgagga tgcttcgatc aacctggagg
     1141 cgatcgcgcg catgagcgcg gatttcccgg acctcgacct gatcctggtc gagtccggcg
     1201 gggacaacct ggccgcgacc ttcagcccgg agctgtcgga tctgacaatc tacgtgatcg
     1261 acgtggccgg cggcgagaag attccgcgca agggtgggcc gggcatcacc cgctcggatc
     1321 ttctgatcat caacaagacc gacctcgcgc cctacgtggg cgccaacctt gacgtgatgg
     1381 ccagcgatgc gaagaaaatg cgcggcgaca agccgttcgt gttcaccaat ctcaggaagg
     1441 gtgagggagt agacatggtg atcgccttca tccggcgcga aggtttgctg gatgacgtga
     1501 agaagaggtg agccgagcct ctggctcgcc cggccatgcg cttgccgttg tcaccctggg
     1561 ccgcgggcga gcaacccgat tgcatccatc acctctcttc gttggacctt actccgcgcg
     1621 cgcttcccgc gtcgacgcca gcagatccca ggccgcgatg aacagggcgg cgatcagcgg
     1681 cccgatgacg aatccgttca aaccgaacag cgccatgccg ccgagcgtgg aaatcagcac
     1741 gacgtaatcc ggcagcttgg tgtccttgcc gaccaggata ggacgcagga tgttgtcgac
     1801 cagcccgatc acgaacacgc cgaacgcgat cagcaccacg ccctgccaga ccgcgccggt
     1861 gacgaggaag tagattgcaa ccgggcccca gatcaggctc gcccccaccg ccggcagcaa
     1921 cgacaggaag gccatcagca ccgcccacag gagcgcgccc tggatgccga gtatccagaa
     1981 gatcaggccg ccgagcgcgc cctgggtgac cgcgaccacg atgttgccct tgacggtggc
     2041 ccggattacg gtcgtgaact tgctgatcag caggcgcttg tgttcctcag acagcgggat
     2101 cgcgtggcgg atgcgcgccg acagcgtggc gccgtcacgc agcaggaaga acagcaggta
     2161 cagcatgatt gcgaagctga ccacgaagtg gaaggtgttc tgcccgatgt tgagcgcctg
     2221 cgaggcgaac agctggctgc cctgcagcgc ggcttcagac aggcgttcgc ggatcgcgta
     2281 gatcgtgccc agctcgtagc ggtcgaggat ctcgaccgcc cacggcggca gcgcattgat
     2341 gatctgcagg aagtaggcgc cgaaatcgag ctggccggtc cggatcatct ggacgacaag
     2401 gttgacttcc ttgaccagcg acatcgtgat caccgacatc gggaagatca cgatgacaag
     2461 cacaaggaac agcgtggtca gcgccgcgag attgcgccgt ccgcgcatgc cggccagcag
     2521 ctgccggtag aacggcgtaa acaggatggc aagcaccgtg ccccagaata ccgccccgta
     2581 gaacggcagc agcacccaga tgaaggcgat ggaaaccgtg atcagcagcg caaaaaagct
     2641 cttttgctgg aaggcgggat tgttcatcgt gattgtgagt gagtgtcgga gcgttgtacc
     2701 acgctcccaa tgccgcgatc aattcggaga aacggggagg atatagccgt cgcgccggcg
     2761 cgcggcgggg gccggtgtcg ttcacatcat gtcatggaat agccgctggg ccccggcttt
     2821 cgccggggcg acgcggtccg gatgtcgtca tcgcgctcgc tccgctggcg acggctaaag
     2881 aagcgcccct ctggcctcga tcttccacac cgcctccacc gttcccgctc cgcttgccag
     2941 gccgcgcacg cagaccagtt cgtcgtacag gttcaccgtc gggtcgcagt ggccaggcac
     3001 cagcagcagg ctctcgccca tcgccggcaa cgcgccgtcg tcgccgccga gcacgccatg
     3061 ctcgtcggag gccttcaggt agccgacgtc gggctgctgc cacggcgccg gcatgccgga
     3121 atccacgctc gacgccttaa gtccggcgtc caccaccgca cggccgggga cggggcgact
     3181 ctgcaccgtg gtcttaacga acagcgcatg ttcgaagcgt atgtcgtcag gaccggcggt
     3241 gttgtccgcg taatcccggt ccatgaagat ataggaaccc ggttgcagtt cgttgtagac
     3301 accggaatcg cgctcatgca cgaagcttcc ggttcctgcg ccggtgatga ttccgaccgg
     3361 gatgccggcc gcctcgaccg cgtcgcgcgc ggcacgcgca tgtgccgcgg cagcggcgat
     3421 ggccgcttgc cgctcctcgg gccggcgcag gtgctgggcg ctgccgtggt agcagtgcag
     3481 acccgcaaag cgcagcgcgg gcagcgcaac aatctgcgcg gccagcgcgc ctgcttgatc
     3541 cgcgcgcacg ccgcagcggc ctgcgcccac gtccacctcg acatagacgt caacgcgcgc
     3601 accgcgcgca tgcgccgccg cgctcaaatc ctgcgcctgg cgcgcgtggt cgacgagcac
     3661 gccgatgcgt gcgcgggtgg ccagttccgc aagacgctgc agcttcgccg gtccaaccac
     3721 ctggttggtg atcagcacgt cctcgatgcc cgcattcacg aacaccgcgg cctcggcgag
     3781 cttctggcag cagatcccga ccgcgcctgc ggcgatctgg cgccgggcga tttccgggca
     3841 tttgtggctt ttcgcatgcg gccgcaggcg cgtgccactg cccgccagcg cggcctgcat
     3901 gcgcgcgagg ttgcggtcga aggcgtcgag atcgagcagg agcgcggggg tgtcgatctg
     3961 cgcggcgggc gcgccgggtt gggctgcgga ccaggggctc atcgcaaaga ggggaagagt
     4021 ggccaaagcg ctcattgtag tgccgccggg gaaagggggc gcagggcgaa ttgccgtacg
     4081 gtgatgcaat gccgccagcc agatcgacgg ccgccatctc acccggtgcg cggaggcgga
     4141 aagaactggg cagtctgcag actgaacggg atttgggcgc gtggcaaagt tatcattccg
     4201 cgcatgaacg cttccgtgct tctcactaac ctcgtcgccg ccttgctgct gcccccgctg
     4261 aacgcagtgc tgctggtgtt gctcggtctc atgctgcgcc ggcgcctgcc gcgggtcggc
     4321 gtggcgctcg tgctgcttgc gctggccgcc ctgaccgtgt tcagcacctt tgccggcgcg
     4381 cgatggttgc acgggccgct cgagcgcacg catccgccgc tgcaggcgcc gcatctgcat
     4441 gatgcgcagg cgatcgtgct gctgggcggc gggcgcatcg aagagctcgc cgagtatggc
     4501 ggggtcgaca ttccctttcc cacggccttc ctgcgcatgc attacgcggc gcgccttcat
     4561 cggcagacgg gactgccggt gctggcgagc ggcggcgtgc ccgatggtgc gggggaatcc
     4621 gaggctgcga tgatggcgcg catgctccac gaggattacg cagtgccggt gcaatggctg
     4681 gaagagcggt cgaacaacac tgcgcagaat gcgaagtaca cggccgcaat cctgttgccg
     4741 caaggcctga ggcggatcct tctcgttacc gatgcgatgc acatggcgcg cgcgacggcc
     4801 gttttcgagc gcgccggctt cgaggtcgtg ccggcgccga ccgcgcggct gtcggagggc
     4861 cccttgcacg cgctcgacct ggtgccgaac ggagaaggcc tgcgccgttc acactacgcc
     4921 ctgcgcgagc ggatcggcca actctggtac cggctgcgcc attgatccgc gcaccggact
     4981 ggtgctgcaa cttttccgag catgtcgatt gtgctaggat aacggctcgt taactcgatt
     5041 gatgccaacg gcatccaaca tcaagggctt actcatcgtt gtaggccctt ttgtttttca
     5101 ggctacgctg aataagtccc gcgatggcgg gcgcccgaaa tcgggatggg ctgcaaggcg
     5161 cacaccgcag cgatagcggt gctatcgcga ggatttgcaa cgccgcagac cgcccgagtt
     5221 cgggatgcgc gacgcgcggg gacttgttca gcgtagcctc ctactcaccg caaggcaccc
     5281 aggtgccggt accgcgggtc gcattgcagt acgcgacctt tgttactaac cacggactga
     5341 aaaacgcagc tgcttgagct gcctgccgtg cccacggcaa tggtccgaac cttgagcaaa
     5401 tcgcatgaca ggcgcgaacc gcgcgggacc gcgcacgccc ctgcatggca cgaccgctcg
     5461 acaagcgccc cgcgctttcg cgccgccatc cggccggtgc atgaccgtgc accgaaacag
     5521 ggaacaaaag gacaactttt tcctctacca gaggtagccc tttcttccca tccgcctccg
     5581 ggcgaaacct gcatgccggt gcggccttga tatatcgacg cgtcccggtg gcagggccgg
     5641 ccgcgcagtc tcctggggag gttaacgatc catgtgtggt attgccggag aactacgttt
     5701 caacaacgag cctgcgaatg tgcgcgcagt gctcgacatg acagacgcgc agaagcgtcg
     5761 cgggccgaac ggcgaaggca tcttcagtct cggcgcgcgc tgcttcggac accgccggct
     5821 gtccatcatg gacttgagcc agcgcgccca ccagccattc gtcgacaaca cgctcggcat
     5881 gggcatcgtc ttcaacggcg cgatctacaa ccatcacgag ttgcgtgcgg aactgcgcga
     5941 gaagggctac cagttcgcat ccaacggcga caccgaggtg atcatcaagg cctggcacgc
     6001 gtgggggccg aaggcgctgg accgcttcta cgg
//
LOCUS       DATLGF010000522         3584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_109321, whole genome shotgun sequence.
ACCESSION   DATLGF010000522 DATLGF010000000
VERSION     DATLGF010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109321"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="VM406_16315"
     CDS             complement(<1..131)
                     /locus_tag="VM406_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL77579.1"
                     /translation="MNLKEVEAFRAVMLTGSMTAAAQQMHTSQPNISRLIARLEKSLG
                     "
     gene            239..1363
                     /locus_tag="VM406_16320"
     CDS             239..1363
                     /locus_tag="VM406_16320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HVL77580.1"
                     /translation="MKTEAADVIVIGGGIHGCSSAYHLASRGARVVLLEADYCGRHAS
                     GVNAGGVRTLGRPVPEIPLALASRELWHRLRELIGDDGNFEASGQLKVAENEGEIAIL
                     KARVDLLASHGFMHEVLVDRETVRELIPSITPTVAAGIWVKDDGHALPYRAVTAFRRA
                     AERCGARVYEQTAVARIERVGTRWKVGTAHGAFEATHVVNAAGAWAANLAAQVDEQVP
                     AKPGGLMLMVTQRVAPFIKPVLGAAVRPLSFKQFDNGTVVIGGALECSVDFEDKHAEL
                     DFSRLSRSARIVTDLFPFLKHVGINRAWSGVEAFTPDKLPVIGPSRKAEGLVHAFGFS
                     ASGFQLGPAVGKMVSELILDGRSSMALDAFAVDRFGVVSG"
     gene            complement(1526..1888)
                     /locus_tag="VM406_16325"
     CDS             complement(1526..1888)
                     /locus_tag="VM406_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HVL77581.1"
                     /translation="MTDITRHDVGARMSEIAVFNNVVYLAGQVAEVNPDAGITRQTEE
                     VLGHIDRLLAKAGTDKSRILQCQIYLKDIADIAAMNAVWDQWVAGGNTPPRATVQAEL
                     ADSRWRIEVVVCAALPVA"
     gene            complement(2146..2499)
                     /locus_tag="VM406_16330"
     CDS             complement(2146..2499)
                     /locus_tag="VM406_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HVL77582.1"
                     /translation="MSTITRLETNQRMSRVVIHNGVVAVGGLTAGDPSQDIKGQTRQV
                     LEKIEGWLAKAGTDKTKLLTAQIWLKDIERDFAGMNEVWDAWAPEGAAPTRATCEAKL
                     ARPELLVEIIVTAAV"
     gene            complement(2742..>3584)
                     /locus_tag="VM406_16335"
     CDS             complement(2742..>3584)
                     /locus_tag="VM406_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006405338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HVL77583.1"
                     /translation="IVDTTDFSDYRRALKHLGGALAGWRYLQKGLMLMRTLKKSGVPF
                     YKSATQLAVEGKDAATALSFEAGGQRHRIDSKLILLHQGVVPNTQFTWALRAKHVWDD
                     AQLCWAPEVDEWGELNIHGIHVAGDGRGIGGAVAAALQGELAGLAVAFGERRIAAEER
                     DRLAAPARAALRDNLNIRPFLDALYRPKLENRVPADDVIVCRCEEVTAGAVREYVGLG
                     CVGPNQTKSFGRCGMGPCQGRMCGLTVTEVIAEARQVSREEVGYYRIRPPIKPITLAE
                     LAGD"
BASE COUNT          569 a         1124 c         1215 g          676 t
ORIGIN      
        1 cccagtgact tttcaaggcg cgcgatcagc cggctgatgt ttggctgcga ggtatgcatc
       61 tgctgcgcgg ccgccgtcat cgatccggtc agcatgaccg cgcgaaacgc ctcgacttcc
      121 ttcaggttca ggttcctgcc catatcactc ctgcatggtg actgttcgaa atgctatttg
      181 acctccgcgc ccggcgctgc cgatactcgc gtgatattcg acttggcagg cacggcagat
      241 gaaaacagaa gcggcggacg tgatcgtcat cggcggcggc atccacggat gctcgagcgc
      301 ataccacctc gcttcgcgcg gcgcacgcgt ggtgctgctg gaggccgact attgcgggcg
      361 tcatgcatcc ggggtgaatg ccggcggcgt gcgcacgctg ggccgcccgg ttccggaaat
      421 cccgctcgcg ctggcctcgc gcgaactgtg gcaccgcctg cgcgagctga tcggcgacga
      481 cggcaacttc gaggcgagcg gccagctgaa ggtggccgag aacgaaggcg agatcgccat
      541 cctgaaggcg cgcgtggact tgctggcgtc gcacggcttc atgcacgagg tgctggtcga
      601 tcgcgagacg gtgcgcgaac tgattccttc gatcacgccg actgtggccg ccggcatctg
      661 ggtcaaggat gacggccatg cgctgccgta ccgtgcggtc accgcgtttc gccgcgccgc
      721 cgagcgctgc ggggcgcgcg tgtacgagca gactgcggtt gcacgcatcg aacgggtcgg
      781 cacgcgctgg aaggtcggca ccgcgcacgg cgcgttcgag gcgacacatg tggtcaacgc
      841 ggccggagcc tgggcggcga atctggcggc tcaggtggac gagcaggttc ccgccaagcc
      901 ggggggactg atgctgatgg tgacgcagcg cgtcgcgccc ttcatcaagc cagtgcttgg
      961 cgccgccgtg cgcccgctgt cgttcaagca gttcgacaac ggcacggtag tgatcggcgg
     1021 cgcgctggag tgcagtgtcg atttcgagga caagcacgcg gagctggact tctcgcggct
     1081 gtcgcgcagc gcgcgcatcg tcaccgacct gtttcccttc ctcaagcatg tcggcatcaa
     1141 ccgcgcctgg tccggcgtgg aggcctttac gcccgacaag cttccggtga tcggtccgag
     1201 caggaaggcc gagggactgg tgcatgcgtt cgggttctcc gcgagcgggt ttcagttggg
     1261 gccggcggtg gggaagatgg tgtcggagct gattctcgat ggacgatcga gcatggcgct
     1321 ggatgcgttt gcggtggatc ggttcggggt agtgtcgggg tgagatcggt tgaaccaggg
     1381 cgagcctgac aaaaacttgt cattccgagc gcagcgagga atctccgttc tgtgacccga
     1441 tgagatccct cgctgcgctc gggatgacaa tgtttagagt tatcgccgtg tgcgccgtgc
     1501 ccgccgtggt tcaatcaacc aaacctcaag ccaccggcaa ggccgcgcaa acgaccactt
     1561 cgatgcgcca gcgcgaatcg gcaagctccg cctgcaccgt cgcacggggc ggggtgttgc
     1621 cgcctgccac ccactggtcc cagacagcgt tcatcgcggc gatgtcggca atgtccttca
     1681 ggtagatctg gcactgcagg atgcggctct tgtcggtgcc tgccttggcg agcaggcggt
     1741 cgatgtggcc cagcacttcc tcggtctgcc tggtgatgcc ggcatccggg ttcacttcgg
     1801 ccacctggcc ggcgaggtag accacattgt tgaagacggc gatttccgac atgcgggcgc
     1861 cgacgtcatg gcgggtgatg tctgtcatgc gtttcaattc ctttttgttt ttgggcggat
     1921 acgtccgcgg actggcgtta agttgggcga atgcttcact tgagcaagta cttcacttgg
     1981 gcgattactc tcgctatgtc gttcccgcgc aggcgggagc ccagttcaga cattggcaga
     2041 tctgcgagcg ccggaactgg gttcccgcct gcgcgggaac gacagggcaa gggttttcgc
     2101 ttttctgaac agcattcgtc cagcagacgc tccgcactcc ctacatcaaa ccgcagccgt
     2161 gacgatgatc tcgaccagca gttccggacg tgcgagcttc gcttcgcagg tggcgcgggt
     2221 cggcgccgca ccttcgggcg cccaggcatc ccacacttcg ttcatgccgg cgaagtcgcg
     2281 ctcgatgtcc ttcagccaga tctgcgcggt cagcagcttg gtcttgtcgg tgccggcctt
     2341 ggcgagccag ccctcgattt tttccagcac ctggcgggtc tgacccttga tgtcctgcga
     2401 cggatcgcct gcggtcaggc cgccgactgc gacgacgccg ttgtgaatca ccacgcggct
     2461 catgcgctgg ttggtttcga ggcgggtaat ggtgctcatc gattctcctt ggtaaaagca
     2521 aagcgccgcc gcgatgggcg cggcgcaggt gggcccgggt gaacgggcgg ttcagggcta
     2581 atgcagttca gataagcatc caaggtatca gtcatcccgg cgaaagccgg gatccagcgg
     2641 cgtcactacg tacatggaag acgctgggcc ccggctttcg ccggggcgac cactgaataa
     2701 cgaagacttg actgaacggc attggcggtt tagcgcagcc tttagtcgcc ggccagttcg
     2761 gccagcgtga tcggcttgat cggcggacgg atgcggtagt agccgacttc ctcgcgcgag
     2821 acctggcgcg cttcggcgat gacctcggtc accgtcaggc cgcacatgcg gccctggcag
     2881 ggacccatgc cgcagcgccc gaaggacttg gtctggttcg gaccgacgca gcccaggccg
     2941 acgtactcgc gcaccgcgcc ggcggtgact tcctcgcagc ggcagacgat gacgtcgtcg
     3001 gccggcacgc ggttctccag cttcggccgg tacagcgcgt cgaggaaggg gcggatgttc
     3061 aggttatcgc gcagcgccgc gcgggcaggg gcggcgaggc ggtcgcgctc ttcggctgcg
     3121 atcctgcgtt cgccgaacgc gaccgcaagc ccggccagct cgccctgcag cgcggccgca
     3181 accgcaccgc cgattccgcg gccgtcaccg gccacgtgga tgccgtggat gttcagctcg
     3241 ccccattcgt cgacctcggg cgcccagcac agctgcgcgt cgtcccagac gtgctttgcg
     3301 cgcagcgccc aggtgaactg ggtgttcggc accacgccct ggtgcagcag gatcagtttc
     3361 gaatcgatgc gatggcgctg gccgccggcc tcgaagctga gcgcggtggc agcgtccttg
     3421 ccttcgacgg caagctgcgt cgcgctcttg tagaacggta cgccgctctt cttcagcgtg
     3481 cgcatcagca tcaggccctt ctgcaggtag cgccagccgg ccagcgcgcc gcccaggtgc
     3541 ttgagcgcgc ggcggtagtc ggagaaatca gtggtgtcga cgat
//
LOCUS       DATLGF010000523         2179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_124850, whole genome shotgun sequence.
ACCESSION   DATLGF010000523 DATLGF010000000
VERSION     DATLGF010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124850"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1523
                     /gene="glgP"
                     /locus_tag="VM406_16340"
     CDS             <1..1523
                     /gene="glgP"
                     /locus_tag="VM406_16340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HVL77584.1"
                     /translation="GFLALGREDVTNTKQGFSMAVLAIRLADSYNAVSELHGEVSRKM
                     WHNIWPDVPPQEVPIQHVTNGIHVRTWLAPDMVYTLDRYLGSRWQMDPTDQAVWEAVE
                     QIPNEELWRAHERCRERLVSWARQMLREQLIRRGAPSDEVSAADEVLDPEALTIGFAR
                     RFASYKRGALLLRDPERLRRLVEDSKRPIQFIFAGKAHPADNEGKELIRAIVQFSRQP
                     SLRRHFVFLENYDMNIARYLVQGVDVWLNTPRRGLEASGTSGMKAAANGVPNCSILDG
                     WWVEGYSPDVGWAIGRGETYSDSSFQDHVESQSLYDLLEKRIVPSFYDRGVDNIPREW
                     IQRMKNCMRKLAPVFNTNRMVREYAEKFYIPSMQRGQKLAENGLAGAVALAQAKDELR
                     HKWGGIRIVGVHTSGNGHYKVGDRMQVEALVDLPDLKPDDIRVQLYAGTMNAAGEIED
                     PQVLNMSHAREMAPGRHLFTGTLECRVSGRQGFALRIVPGKVDMATPFEPGLIIWN"
     gene            1593..>2179
                     /locus_tag="VM406_16345"
     CDS             1593..>2179
                     /locus_tag="VM406_16345"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HVL77585.1"
                     /translation="MPFIRRSRPWQRIAGSATSQEIYLNRRSFLAALGAGAIGAGVAL
                     AAEGDTISAASSRPGVARKSKFELGRELTDATVAARYNNFYEFGIGKLDPARRAHSLK
                     TTSWTIEIAGLVKQPRTLDLDDLLQRMPLEERLYRFRCVEAWALAAPWVGFPLQLLID
                     QVEPLSSAKFVRFVTADRPDQMPGIKEQPWLPWPY"
BASE COUNT          398 a          687 c          714 g          380 t
ORIGIN      
        1 aaggattcct cgccctcggc cgcgaggatg tgaccaatac caagcagggt ttttcgatgg
       61 cggtgctggc gatccggctg gccgacagct acaacgcggt gagcgaactg catggcgagg
      121 tgtcgcgcaa gatgtggcac aacatctggc ccgatgtccc gccgcaggaa gtgccgattc
      181 agcacgtcac caacggcatt cacgtgcgca cgtggctggc gccggacatg gtttacactc
      241 tggaccgcta cctcggcagc cggtggcaga tggatccgac cgaccaggcg gtgtgggaag
      301 cggtcgagca gattccgaac gaggagctgt ggcgcgccca tgagcgctgc cgcgagcgcc
      361 tggtgagctg ggcgcggcag atgttgcgcg agcagttgat ccgacgcgga gcacccagcg
      421 acgaggtgag cgcggccgac gaggtgctcg atccggaagc cctgacgatc ggctttgccc
      481 gtcgctttgc ctcgtacaag cgaggtgcgc tgctgctgcg cgatccggag cggctgcggc
      541 gtttggttga ggacagcaag cgtcccatcc agttcatctt cgccggcaag gctcatccag
      601 ccgacaacga gggcaaggag ctgatccgcg cgatcgtgca gttttcacgg cagccttcgt
      661 tgcggcggca cttcgtcttt ctcgaaaact acgacatgaa catcgcccgc tacctggtgc
      721 agggcgtgga cgtgtggctg aatacccctc gccgcggact tgaagccagc ggcaccagcg
      781 gcatgaaggc cgccgccaac ggcgtgccca actgctccat cctcgatggc tggtgggtcg
      841 aaggttactc gcctgatgtc ggctgggcga tcggccgcgg ggaaacctac agcgacagca
      901 gtttccagga ccacgtcgag agccagtcgc tgtacgacct gcttgaaaag cggatcgtgc
      961 cgtcgttcta cgatcgcggc gtggacaaca ttccgcgcga atggattcag cggatgaaga
     1021 actgcatgcg caagctggcg cccgtcttca acaccaatcg gatggtgcgg gagtatgcgg
     1081 agaagttcta catcccttcg atgcagcgcg ggcagaagct ggccgagaac ggtttggccg
     1141 gggcagtcgc gctggcgcag gcgaaggatg aactgcgcca caagtggggc ggcatccgca
     1201 tcgtcggcgt ccacacgagc ggcaacggcc actacaaggt tggcgaccgc atgcaggtcg
     1261 aagcgctggt cgatctgccg gatctgaagc ccgacgacat ccgcgtgcag ctctacgccg
     1321 gcacgatgaa cgccgccggt gaaatcgaag acccgcaggt gctgaacatg tcgcatgcgc
     1381 gcgagatggc gccggggcgg cacctgttca ccggcacgct cgagtgccgc gtgagtggcc
     1441 ggcagggatt cgccctgaga atcgttcccg ggaaggtcga tatggcgacg ccgttcgagc
     1501 ccggcttgat catctggaac tgacattcac tgccgatcac gctgcggtgc gcgaaccgtg
     1561 catttcacac cccctgtccg taccattggc gcatgccttt cattcgacgt tcgcgcccct
     1621 ggcagcgcat cgcaggcagc gcgacatctc aggagattta cctcaaccgc cgctccttcc
     1681 tcgccgcact tggcgccggc gcgattggag cgggcgtagc gctggccgcg gaaggcgaca
     1741 cgatttctgc cgcgtcgagt cggcctggcg ttgcacgcaa aagcaaattc gagctcggcc
     1801 gcgagctcac cgacgccacc gttgccgccc gctacaacaa cttctacgaa ttcggcatcg
     1861 gcaagcttga tccggcgcgg cgcgctcact cgctgaaaac cacgtcctgg acgatcgaga
     1921 tcgccggcct ggtcaaacag ccgcgcacgc tggatctgga tgatctgctt cagcgcatgc
     1981 cgctggaaga gcggctgtat cgctttcgct gcgtcgaggc gtgggcgctg gcggcgccat
     2041 gggtaggctt cccgctccag ctcctcatcg accaggtcga gccgctcagc tccgcaaagt
     2101 tcgtgcgctt cgtcacggct gatcgcccgg accagatgcc gggtatcaag gaacagccgt
     2161 ggctgccgtg gccttactg
//
LOCUS       DATLGF010000524         5465 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_124873, whole genome shotgun sequence.
ACCESSION   DATLGF010000524 DATLGF010000000
VERSION     DATLGF010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124873"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(542..1459)
                     /locus_tag="VM406_16350"
     CDS             complement(542..1459)
                     /locus_tag="VM406_16350"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL77586.1"
                     /translation="MDDRVLRAFCDIAENGSLTRAAAILGEKQSALSRRVAALEDELG
                     GRLFYRTGRGMTLTDLGERLLPRIQSILAETDALVRDAQGERDSPTGVVEIGVVPALS
                     QPLVSGLCKRLRVRYPGIRLRAFEGYSGQVEEWLANGKVELAVCNSYGRGMLPGAQLL
                     LQSGVALVGPHQMLDGIGSETAFPALRDVPLVLPTRPNVLVSKLVDIAARHRFTLQIA
                     LEAGTGSLIRDAVQHVGLCTVLPLVIAERDFRAPDYVISRLIKPSLQQKAWLLASTQR
                     PVSRAARLVQRMISDIAAQAAYERLVQDH"
     gene            1545..2759
                     /locus_tag="VM406_16355"
     CDS             1545..2759
                     /locus_tag="VM406_16355"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HVL77587.1"
                     /translation="MPTDTAARSGPLSGLKVVDLSTVVAGPFAAGLLADFGADVIKVE
                     LPGHGDALRALAPHKDGVPLWWKVTNRNKKGVTLDLRREEARPLLARLIAEADVLVEN
                     FRPGTLDGWGITKDWLHGHNPRLTILRVTGFGQDGPYANRPGFARVFESMSGFTQICG
                     EEGGPPLHLGYPISDAVGGLFGALGVLAALLRLKTHPEDGGQEIDCSVTEAMLRVLEF
                     LPIEYDQLGKVRTRSGNRSQYAAPGNIYQTRDGRWASIAASTQSIFERLCDALDLREL
                     LDDERFATNRMRVVHRDEVDRIVGGAIAQLTLAELGERFERYEVGFSPIYDIADVFAD
                     PHFAARNTLVKVTDSELGEVRMQGVVPRFSDTPGAVHAAGPALGEHNAGVWTSLGLSE
                     DEVAALREQRVI"
     gene            2839..3807
                     /locus_tag="VM406_16360"
     CDS             2839..3807
                     /locus_tag="VM406_16360"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVL77588.1"
                     /translation="MKALLVRLAVVMVAMWGAMAPAQAQGSKVTRIVVGFPPGQATDL
                     VARLIAERLTVSLGHSVIVENKPGQGGSIALAQLAKSPADGSVLSLSALAAYVVNPYL
                     YPTVRYDSVKDLAPIGMVAELPMLLVVHPSVPAHTLPELIAYAKANPEALAHSSSGNG
                     TLSHLMMEDLKRRAGITILHTPYQGSAPAMTDLVAGRVQVGLDTIAATQSFVKDGKLR
                     LIAAGTPRRLAAFPEAPTVAEQGFEGFEAAAWIALSAPAGTPLEVRTKINDAVVAALN
                     TPEFQERLAALGAVPRPGTVEEFSAYLRSEQQRWKSIVERSGARVD"
     gene            3812..4954
                     /locus_tag="VM406_16365"
     CDS             3812..4954
                     /locus_tag="VM406_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HVL77589.1"
                     /translation="MGALSGVRVLEFEAIGPAPFCGMMLADMGADVLLVDRPGDAREG
                     FGRERWYDVMFRGRRSLALDLKSEAAVETVLMLAEKADVLIEGYRPGVMERLGLGPDI
                     LMARNPRLVYGRVTGWGQDGPLAQRAGHDINYLALSGVLHAIGREGGAPVPPLNLVAD
                     FGGGGMALAFGVASALLQARASGRGQVVDAAMTEGAGLLATMISGMQAAGRWNEARGT
                     NLLDSGAPWYDCYETLDGRHMAVGAIEPKFYAQLLQGLGLAQEALPAQRDRAGWPVLR
                     ERFAAAFRGKTLDEWAQVFDDSDACVTPVLTFAEARRHPHMVARRAHGEIGAIVQPVP
                     APRMARTPSRVAGAPPRRGEGAREALQDWQLSPEQLARLAQLGVAA"
     gene            4991..>5465
                     /locus_tag="VM406_16370"
     CDS             4991..>5465
                     /locus_tag="VM406_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HVL77590.1"
                     /translation="MFKALLLEQEGKQTIAQLVEMDEARLPEGDVMVRVEYSTLNYKD
                     GLAITGKSPVVRAWPMVPGIDFAGTVETSTSERFKAGDKVVLNGWGVGELHWGGLAEK
                     ARVPAEYLIGLPDTLSTRDAMAIGTAGYTAMLAAQALERHGVMPQSGPVLVTGANG"
BASE COUNT          920 a         1741 c         1926 g          878 t
ORIGIN      
        1 ggaagatgcc tgccgcaaag atcaatgcga gcacgacgta gccgaggctg aggagcaagg
       61 cttcccgtac cgagatttcc cgggcgccac ggtgcaggac gccgaggtcg aaagccagca
      121 gtgccagcac gaagatgttg aagcctatcc aggcccagat catgtccacg ttgaatcttt
      181 ccggttgaat gagaggcaat atgatcggcc atggtacgcc tttcgccggt gcttcttaca
      241 tcgccgattt cattagtctg ggttcgaaaa aacaggaatg agggggcatc atgttgtcgt
      301 cccgaacgtc gtgagggcgg cccgcgccag ggatctcatc ggctcggcaa gcgaggacgc
      361 aaggcaatcg gataagattc ctcgcttcgc tcggaacgac aggcttgggg gcagtcgcca
      421 aaattgctgt cttcccgaac gcagtgaggg cggcccgcgt cagggatctc atcggccagg
      481 catgcgacgc cacgcagcga gcggacgatt tcggaaacac ggaccgtgcg cggcaacacg
      541 ttcagtgatc ctgcacaagc cgctcatacg ccgcctgtgc cgcgatgtcc gagatcatcc
      601 gctgcaccag ccgcgccgca cgcgataccg gacgttgcgt ggacgctagc agccatgcct
      661 tctgctgcag cgacggcttg atcaatcgcg agatcacgta gtcgggcgcc cggaaatcgc
      721 gctcggcgat gaccagcggc agcaccgtgc acaggccgac atgctggacc gcgtcgcgga
      781 tcagggagcc ggtaccggcc tccagcgcga tctgcaaggt gaagcggtga cgggcggcga
      841 tgtcgacgag cttcgaaaca agcacgttcg gccgggtcgg caataccagc ggcacgtcac
      901 gcagcgccgg gaatgccgtt tcgctgccga tgccatccag catctggtgc ggcccgacca
      961 gtgcgacacc ggactgcagc agcagctgcg ccccgggcag catgccgcgg ccataactgt
     1021 tgcagaccgc gagctcgacc ttgccgttcg ccagccactc ctccacctgc ccgctgtagc
     1081 cttcgaatgc gcgcaggcgt atccctggat accgcacccg cagacgcttg cacagcccgc
     1141 tgaccaaggg ctgcgacagc gccggcacga cgccgatttc gaccacgccg gtgggactgt
     1201 cccgctctcc ctgcgcatcg cgcaccagcg cgtcggtctc ggcgaggatc gactggatgc
     1261 gcggcagcag gcgttcgccg agatcggtca gcgtcatgcc gcgcccggta cggtagaaca
     1321 ggcggccgcc cagctcatcc tcgagcgccg ccaccctgcg gctgagcgcc gactgcttct
     1381 cccccaggat cgcagcggca cgggtgaggc tgccgttttc cgcgatgtcg caaaaggccc
     1441 gcagcacgcg gtcgtccatg tctcctccgg tatgcagaat ctgcaaagct ggtttatctg
     1501 atttgatgat accggatcgg caaagcggcg cttatagtgg ttccatgcct accgataccg
     1561 cagcccgcag cgggcctctc tccggattga aggtcgtgga cctgtccacc gtggtcgccg
     1621 ggccgttcgc cgccggcctg cttgccgatt tcggcgccga tgtgatcaag gtcgagctgc
     1681 ccggccacgg cgacgcgttg cgcgcgctgg cgccacacaa ggacggtgtg ccgctgtggt
     1741 ggaaggtcac gaaccggaac aagaaaggcg tgacgctgga cctgcgccgc gaggaagcgc
     1801 ggccgctgct ggcgcggctc atcgccgagg ccgacgtact ggtcgagaac ttccggcccg
     1861 gcacgctgga tggctggggc atcacgaagg attggctgca tggccacaat ccgcgcctca
     1921 cgatcctgcg tgtgaccggc ttcgggcagg acggtcctta cgcgaaccgg cccggctttg
     1981 cgcgtgtgtt cgagtcgatg agcggcttca cccagatctg cggcgaggaa ggcggcccgc
     2041 cgctgcacct cggctacccg atctcggatg cggtgggcgg actgttcggc gcgctcggcg
     2101 tgctggccgc gctgctgcgc ttgaagacgc acccggagga cggtggccag gaaatcgact
     2161 gctccgtcac cgaggcgatg ctgcgcgtgc tggagttcct gccgatcgag tacgaccagc
     2221 tcggcaaggt gcgcacccgc tcgggcaacc gcagccagta cgcagcgccc ggaaacatct
     2281 atcagacgcg cgacggacgc tgggcgtcga tcgccgcgtc cacccagagc atctttgagc
     2341 gcctgtgcga cgcgctggac ctgcgcgagc tgctggacga cgagcgcttt gcgacaaacc
     2401 gcatgcgcgt cgtgcaccgc gacgaagtcg atcgcatcgt tggcggcgcg atcgcgcagc
     2461 ttacgctggc cgaactgggc gagcgctttg aacggtacga ggtcggtttc tcgccgatct
     2521 acgacatcgc cgacgtgttc gccgatccgc acttcgcggc gcgcaacacg ctggtcaagg
     2581 tgacggacag cgagctcggc gaagtcagga tgcagggcgt ggtgccgcgc ttttcggata
     2641 cgccgggcgc ggtgcatgcg gccgggcctg cgctgggtga gcataacgcc ggcgtgtgga
     2701 cgtcgctcgg gctgagcgag gacgaggtgg ccgcgctgcg cgagcaaaga gtgatctgag
     2761 taggaaggac gcaatcatcg tcgacgaatc gggacaaaga gcccgaagcg acaattcaac
     2821 gagagaagga gacaagcgat gaaggcatta ctggtcaggc tggcggtggt gatggtggcg
     2881 atgtggggcg caatggcgcc cgcgcaggcg cagggatcga aggtaacccg catcgtggtc
     2941 ggcttcccgc ccggccaggc gaccgacctg gtggcgcgcc tgatcgccga acgcctcacc
     3001 gtgtcgctcg ggcattcggt gatcgtcgag aacaagccgg gccagggcgg cagcatcgcc
     3061 ctcgcccagc ttgcgaagtc gccggccgac ggcagcgtgc tgagcttgtc cgcactggcc
     3121 gcctacgtcg tcaatcccta tctgtacccg accgtgcgct acgacagcgt gaaggacctt
     3181 gcgcccatcg gcatggtggc ggagctgccg atgctgctgg tcgtgcatcc gtcggtgccc
     3241 gcgcacacgc tgccggagct gattgcgtac gcaaaggcca atccggaggc gctcgcgcat
     3301 tcctcgtccg gcaacggcac cctctcccac ctgatgatgg aggacctgaa gcggcgcgcc
     3361 ggtatcacga ttctgcacac accatatcag ggcagcgccc ccgcgatgac cgatctggtc
     3421 gccgggcgcg tgcaggtcgg gctggacacg atcgcggcca cgcagtcctt cgtcaaggat
     3481 ggcaagctgc ggctgatcgc ggccggcaca ccgcgcaggc ttgcggcctt cccggaggcg
     3541 cccacggtgg ccgaacaggg cttcgaaggc ttcgaggcgg cggcctggat cgcgctgtcc
     3601 gcgccggccg gtacgccgtt ggaggtgcgc acgaagatca acgatgcggt ggttgccgcg
     3661 ctgaacacgc ctgagttcca ggaacgcctg gcggcactcg gcgcggtgcc gcgcccgggc
     3721 acggtggagg aattcagcgc ctacctgcgc agcgagcagc agcgctggaa gagcatcgtc
     3781 gaacgctccg gcgcgcgggt ggactgagcg catgggagcg ctttccggcg tgcgcgtgct
     3841 cgaattcgag gcgatcgggc ccgccccctt ttgcggcatg atgctggccg acatgggcgc
     3901 cgacgtgcta ctcgtggatc ggcccgggga tgcgcgcgaa ggctttggcc gcgagcgctg
     3961 gtatgacgtg atgtttcgcg gacgccgctc gctggccctg gacctgaagt ccgaagcggc
     4021 cgtcgaaacg gtgctgatgc tggcggaaaa ggccgacgtg ctgattgaag gttaccggcc
     4081 cggcgtgatg gagcggctcg gtctggggcc ggacatcctg atggcgcgca atccccggct
     4141 ggtctacggg cgcgtgaccg gctggggaca ggacgggccg cttgcacagc gggccggaca
     4201 tgacatcaac tacctggcgc tgtccggcgt cctgcacgcg ataggtcgtg aaggtggtgc
     4261 gccggtgccg ccgctcaatc tcgtcgccga cttcggcggc ggcggcatgg cgctggcgtt
     4321 cggcgtcgcc tcggcgctgc tgcaagcgcg ggcgtccggc cgcggacagg ttgtcgacgc
     4381 tgcgatgacg gaaggcgccg gcctgctggc gacgatgatc tcaggcatgc aggcggcggg
     4441 ccgctggaac gaggcgcgcg gtaccaacct gctcgattcc ggcgcgccct ggtacgactg
     4501 ctacgagacc ctcgacggcc gccatatggc cgtcggcgcg atcgagccga agttctacgc
     4561 gcagctgctg caggggctgg gactggcgca ggaggcgctg ccggcccagc gtgatcgcgc
     4621 gggctggccg gtgttgcgtg agcgtttcgc cgctgcgttc cgcggcaaga cgctggatga
     4681 atgggcgcag gtgttcgacg acagcgatgc ctgtgtgacg ccggtgctga ctttcgcgga
     4741 ggcacgccgg catccgcata tggtggcgcg gcgcgcccac ggcgagatcg gcgcgattgt
     4801 tcagcccgta cccgcgccgc gcatggcgcg cactccgtcc cgtgtcgccg gcgcgccgcc
     4861 tcggcgcgga gaaggtgcgc gcgaggcgct gcaggactgg cagctgagtc cggagcagct
     4921 cgcgcggctc gcacagctgg gtgtggccgc ctgacaattt ctcaagcagg tttttaccgg
     4981 gaggatacga atgttcaagg ctttgttgct ggagcaggaa ggcaagcaga ccatcgcgca
     5041 actggtcgag atggatgagg cgcggctgcc cgagggcgat gtcatggtac gcgtcgagta
     5101 ttcgacgctc aactacaagg acgggctcgc gatcaccggc aagagcccgg tggtgcgggc
     5161 atggccgatg gtgcccggca tcgactttgc cggaaccgtg gaaaccagta cgagcgagcg
     5221 cttcaaggcg ggcgacaagg tggtgctgaa cggctggggc gtcggcgagt tgcattgggg
     5281 cgggctggcc gaaaaggcgc gggttccggc cgaatacctg atcggcttgc cggacacgct
     5341 gagcacccgc gacgcgatgg ccatcggcac cgccggctat accgcgatgc tggccgcgca
     5401 ggcgctcgag cgtcatggcg taatgccgca atcgggaccg gtgctggtta ccggcgcgaa
     5461 cggcg
//
LOCUS       DATLGF010000525         5425 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_109739, whole genome shotgun sequence.
ACCESSION   DATLGF010000525 DATLGF010000000
VERSION     DATLGF010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109739"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..952
                     /gene="minD"
                     /locus_tag="VM406_16375"
     CDS             137..952
                     /gene="minD"
                     /locus_tag="VM406_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HVL77591.1"
                     /translation="MAKIIVVTSGKGGVGKTTSSASFASGLALRGHKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVINKEATLNQALIKDKHCDNLFVLPASQTRDKDALTEAGV
                     ERVLNDLAGMGFEYIICDSPAGIEHGAVMALTFADEAVVVTNPEVSSVRDSDRILGII
                     QAKSRRALHGQEPVKEHLLITRYMPKRVEAGEMLSYTDVQEILRIPLIGIIPESESVL
                     HASNQGTPAIHMDGSDVSEAYKDVVARFLGNEVPLRFTAYEKPGLFQRLFKGK"
     gene            954..1217
                     /gene="minE"
                     /locus_tag="VM406_16380"
     CDS             954..1217
                     /gene="minE"
                     /locus_tag="VM406_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HVL77592.1"
                     /translation="MSLLSFLFNNKPKSASAAKERLQVIIARERNGRDGPDFLPALHK
                     ELIEVISKYAKVDPEDIKISLDRQGNLEVLDVNVVLPDVVPGK"
     gene            complement(1334..1660)
                     /locus_tag="VM406_16385"
     CDS             complement(1334..1660)
                     /locus_tag="VM406_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77593.1"
                     /translation="MLRGNEIQQQYSQVEAAIHQAAQRCESTGSVPMDLKDSIQRLEQ
                     QTSQARDVILSQDENRIRQCIDDMEEIGDQAKAACENAGQLDPQLRDAVMQAHQQLSN
                     LKHQLH"
     gene            1896..3989
                     /locus_tag="VM406_16390"
     CDS             1896..3989
                     /locus_tag="VM406_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HVL77594.1"
                     /translation="MSRAEMEKLGWDSCDVILVTGDAYVDHPSFGMALVGRLLEKQGF
                     RVGIISQPDWHSAEPFRALGKPNLYFGITAGNMDSMINRYTSDRKIRSEDAYTPNGDP
                     NKRPDRAVIVYAQRAREAFPDANIVIGSIEASLRRIAQYDYWSDKVRRSVLPDSKADI
                     LLYGNAERALVALTHRLAAGEAIRNIRDLRGTAFMVPAGWKPAPDWSEANSTRYDTPG
                     RIEPHPDPYEMKAKEPATCGTDAKPAAQPVVMLSREERQALLKEQRAKTVVRLPSYEA
                     VKDDPVLYAHASRVLHLESNPGNARALVQAHGDRDVWLNPPPLPLAMDEMDGVYDLQY
                     ARSPHPAYGRARIPAWEMIRFSVNIMRGCFGGCTFCSITEHEGRIIQSRSERSILREI
                     ELVRDKTKGFTGTISDLGGPTANMYRLACKDKKIEESCRRLSCVYPTICSNLNTDHSK
                     LIQLYRKARAIPGIKKILVSSGLRYDLAVKSPEYVKELVSHHVGGLLKIAPEHSEEGP
                     LTKMMKPGMGSYYKFKEMFERYSKEAGKEQYLIPYFIAAHPGTTDEDMLNLAVWLKQN
                     NFQLDQVQTFLPTPMAMATAMYHGRKNPLHKISADSEAVETVRSGKIRRLHKAFLRYH
                     DPENWPILREALVRMGRSDLIGNSEHHLVPRSDGAPAARMRKPVGGKAARPAAPRPIL
                     PQKGKRPMPQKNGRK"
     gene            4260..4403
                     /locus_tag="VM406_16395"
     CDS             4260..4403
                     /locus_tag="VM406_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77595.1"
                     /translation="MNDLTSERTDTAISRGFNFNKLQAALCIASSSAVSLGLIYLAVT
                     RLF"
     gene            complement(4675..>5425)
                     /locus_tag="VM406_16400"
     CDS             complement(4675..>5425)
                     /locus_tag="VM406_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77596.1"
                     /translation="LQAIPAIRSAFIPLADDGRDSRWSKPFSLIKADFEPWLSFYAGY
                     CDSRLFLAGSSVETALVPDYPVQALFSLRALDARLLHEPLVQIDAPVEYFDASRAPRA
                     GRVAARSESLARAADAMHRSQLRRAVERGRDMGNALHEALLGNGLSNLIGAWEKRHGR
                     KPNPHELHLMLTTGRAEPVKQAPPVVEAQAPARPAPPATRPAAAAASAYQETATSTEG
                     DAPAARSKWWYLFHAAGLVAAGAMLYILAAA"
BASE COUNT         1056 a         1779 c         1652 g          938 t
ORIGIN      
        1 cctatcgcac cttcgacggc ggcctgcccg ccgaagtggc aaagaaatgc gcgcaggtca
       61 ggcttgcggg cgaccggatc gatgtgatat ccattcgcgc ctgaaacgtt tcaaggaata
      121 caagaaagga gtttgtgtgg caaagatcat cgttgttacg tccggcaagg gcggcgtggg
      181 caagaccacc tcgagcgcca gctttgcctc cggccttgcg ctgcgcgggc acaagaccgc
      241 tgtcatcgac ttcgacgtcg gcctgcgcaa cctcgacctg atcatgggct gcgagcgccg
      301 cgtcgtctac gacctgatca acgtgatcaa caaggaagcc acgctcaacc aggcgctgat
      361 caaggacaag cactgcgaca acctgttcgt gctgccggct tcccagaccc gcgacaagga
      421 tgcgttgacc gaggccggcg tcgagcgcgt gctgaacgac ctggcaggca tgggcttcga
      481 atacatcatc tgcgactcac ccgccggcat cgagcacggc gcggtgatgg cgctgacctt
      541 cgccgacgag gctgtcgtcg tcaccaaccc ggaagtctcg tcggtgcgcg actcggaccg
      601 cattctcggc atcatccagg cgaagtcgcg ccgtgcactg cacggccagg agccggtcaa
      661 ggagcacctg ctgatcaccc gctacatgcc caagcgcgtg gaagccggcg aaatgctgtc
      721 ctacaccgac gtgcaggaaa tcctgcgcat tccgctgatc ggcatcatcc cggagtccga
      781 gtctgtgctg catgcgtcga accagggcac gccggccatc cacatggatg gcagcgacgt
      841 gtccgaggcc tacaaggacg tggtggcccg cttcctcggc aacgaagtgc cgctgcgctt
      901 caccgcctat gaaaagccgg ggctgttcca gcgactgttc aaggggaagt gacatgtcat
      961 tgctttcctt cctgttcaac aacaagccga aaagcgccag cgccgcgaag gaacgcctgc
     1021 aggtgatcat cgcgcgcgag cgcaacgggc gcgacggccc ggacttcctg cccgcgctgc
     1081 acaaggaact gatcgaggtg atttcgaagt acgcgaaggt cgacccggaa gacatcaaga
     1141 tctcgctcga ccgccagggc aacctcgaag tgctggatgt gaacgtggtg ctgccggacg
     1201 tggtgccggg gaagtaaggg gccggcacca ggactgaacc cggggaagag ccccgaacat
     1261 gtcgttcccg cgcaggcggg aacgacaagc acgaaactga agcttgagcg gacgccttgc
     1321 gaccgctccc gcatcaatgc agctgatgct tcagattcga caactgctgg tgtgcctgca
     1381 tcaccgcatc gcgcagttgc ggatccagtt gtccggcatt ctcgcaggcc gccttggcct
     1441 ggtcgccgat ctcttccatg tcgtcgatgc actgccggat gcgattctca tcctgcgaca
     1501 ggatcacgtc gcgcgcctgc gaagtctgct gctcgagccg ttggatcgaa tccttcagat
     1561 ccatcggcac cgaaccggtc gactcgcaac gctgcgcagc ctggtgaatg gccgcttcga
     1621 cctggctgta ctgctgctga atctcgttgc ctcttaacat gtggcacctc ctcgctgttg
     1681 gttgaaggtc aacaggacct gttggatgag cggcgggatc agcgagttcc acccgccccg
     1741 cggcaacttg cggccggatt ttctgtccca cttcgttagc cctttttcgc atacccgttt
     1801 ccggtacaat ccgccgcacc atgcagaccc ccgcccacct gttctcctat ccgccccatc
     1861 gccccgacta cacgcagcct gtgccgttcc tgccaatgtc gcgcgcggaa atggagaagc
     1921 tcggctggga cagctgcgac gtgatccttg tgaccggcga cgcctatgta gaccatccga
     1981 gcttcggcat ggcgctggtc gggcgcctgc tggaaaagca gggtttccgc gtcggcatca
     2041 tcagccagcc ggactggcat tctgcggagc cgtttcgtgc cctcggcaag ccgaacctgt
     2101 atttcggcat caccgccggc aacatggatt cgatgatcaa tcgctacacc tcggaccgca
     2161 agatccgctc cgaggacgcg tacacgccga acggcgatcc gaacaagcgc cccgaccgcg
     2221 cggtgatcgt ctacgcgcag cgcgcgcgcg aggccttccc ggatgcgaac atcgtcatcg
     2281 gcagcatcga agccagcctg cgccgcatcg cccagtacga ctactggtcg gacaaggtgc
     2341 gccgctcggt gctgcccgat tcgaaggccg acatcctcct gtacggcaat gcagaacgcg
     2401 cgctggtcgc actcacgcac cggctggccg ccggcgaagc gatcaggaac atccgcgacc
     2461 tgcgcggcac cgccttcatg gtgcccgccg gttggaagcc cgcgcccgat tggagcgagg
     2521 caaactcgac ccgctacgat acgccgggcc ggatcgagcc gcatcccgat ccgtacgaga
     2581 tgaaggcgaa ggagcccgcc acctgcggca ccgacgccaa gccggccgcg cagccggtgg
     2641 tgatgctctc gcgcgaggaa cgccaggccc tgttgaagga gcagcgcgcc aagaccgtgg
     2701 tgcgcctgcc gtcctacgag gcggtcaagg acgatccggt gctgtacgcg cacgcgtcgc
     2761 gcgtgctgca cctggaatcg aaccccggca atgcgcgcgc attggtgcag gcgcacggcg
     2821 accgcgacgt gtggctcaat ccgccgccgc tgccgctggc catggacgag atggacggcg
     2881 tctacgacct gcagtatgcg cgctcacccc atcccgccta cggccgtgca cgcattccgg
     2941 cctgggagat gatccgcttc tcggtcaaca tcatgcgcgg ctgtttcggc ggctgcacgt
     3001 tctgctcgat caccgagcac gagggccgca tcatccagag ccgttccgag cgttcgatcc
     3061 tgcgcgagat cgaactggtg cgcgacaaga ccaagggctt caccggtacg atctcggacc
     3121 tcggcgggcc gaccgcgaac atgtatcgcc tcgcctgcaa ggacaagaag atcgaggaat
     3181 cctgccgccg cttgtcctgc gtgtacccga ccatctgcag caacctgaac accgatcatt
     3241 cgaagctgat ccagctgtac cgcaaggcgc gcgcgatccc cggcatcaag aagatcctgg
     3301 tgagttccgg cctgcgctac gacctggcgg tgaagtcgcc cgagtacgtg aaggaactgg
     3361 tgagccacca tgtcggcggc ctgctgaaga tcgcgcccga gcattccgag gaaggcccgc
     3421 tgacgaagat gatgaagccg ggcatgggct cgtactacaa gttcaaggaa atgttcgagc
     3481 gctactcgaa ggaagcggga aaggagcagt acctgatccc ctacttcatc gccgcgcatc
     3541 cgggcacgac cgacgaggac atgctgaacc tcgcggtctg gctcaagcag aacaacttcc
     3601 agctcgatca ggtgcagacc ttcctgccga cgccgatggc gatggcgacc gcgatgtacc
     3661 acgggcgcaa gaacccgctg cacaagattt cggcggattc ggaagcggtg gaaacggtgc
     3721 gcagcggcaa gatccgacgc ctgcacaagg ccttcctgcg ctatcacgat ccggagaact
     3781 ggccgatcct gcgtgaagcg ctggtgcgca tgggcaggag cgatctgatc ggcaacagcg
     3841 agcaccacct ggtgccgcgc tctgacgggg cgccggccgc gcgcatgcgc aagccagtcg
     3901 gcggcaaggc ggcgcggccc gcagcaccgc gcccgattct gccgcaaaaa ggaaaacggc
     3961 cgatgccgca gaagaacggg agaaagtagc gagaaccgat attcgcgccg ggaatatcag
     4021 gtatgagaac aataaagcag acaagggtcc tgccgctcgc tatagttaca cctgtctcct
     4081 ccaactcttc ttgatttgga tttacccgca agccgcaagg cctgcgggtt ttttttttgg
     4141 cgagcgtccg gcagttccgc tcattcaagt tttcttacaa aaaagttgct gaaccaacta
     4201 ttgctttgcg ttatcgggtg tttacaatgg tcaataccgt tgaccccctt ggagccgtaa
     4261 tgaacgacct caccagcgag cgcaccgaca ccgcgatatc gcgcggcttc aatttcaaca
     4321 aactgcaggc cgcgctctgc attgccagct cctccgccgt cagccttggc ctgatctacc
     4381 tggccgtcac ccgcctcttc taagaagccc ccaacaaaaa gccgcttgcg gcattgcgcc
     4441 tccttgccgc actacacgta ctgtgtgcct cggcgcgaca tgccatcggc attttgtttg
     4501 gacttccaag tccgcgcacg catcgcgcat tcaaagcgga cctcggatcc aaatgccttc
     4561 cactgaatcg atttccttcg ctatgtcgcc ccctgcgcgg gggcgacagg cttgaagcaa
     4621 tcgcttcagt gaacagccat tgactcctcc gattttccca accgcgtact tcgtctacgc
     4681 agccgcaagg atgtaaagca tcgcgccggc cgcgaccagg ccggccgcgt ggaacagata
     4741 ccaccacttc gagcgggcgg ccggggcatc gccttccgtg ctcgttgccg tctcctggta
     4801 agcactggca gcggcggccg ccgggcgcgt tgccggcggg gccggccgcg cgggtgcctg
     4861 cgcctcgacg acgggcgggg cctgtttgac gggctcggca cgcccggtcg tcagcatcag
     4921 gtgcaattca tgcgggttcg gtttgcggcc atgccgcttc tcccacgcgc cgatcagatt
     4981 cgacaatcca tttccaagca gcgcctcgtg caacgcattg cccatgtcgc gcccgcgttc
     5041 gacggcacgc cgcaactggc tgcgatgcat cgcatcggcg gcccgggcga gggattcgct
     5101 gcgagcggcc acgcggcccg cgcggggagc gcggctggcg tcgaaatatt cgaccggcgc
     5161 gtcgatctgc accagcggct catgaagcag acgcgcatcc agcgcgcgca atgagaacag
     5221 tgcctggacc ggataatcag gcaccaatgc ggtctcgacc gaggaaccgg ccaggaacag
     5281 gcgcgagtcg caatagccgg cgtagaacga gagccagggt tcgaaatcgg ccttgatcag
     5341 cgagaagggt ttgctccagc ggctgtcccg gccatcgtcg gcaagcggaa tgaacgcgga
     5401 gcggatggcg ggaatggcct gcagc
//
LOCUS       DATLGF010000526        20938 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_125109, whole genome shotgun sequence.
ACCESSION   DATLGF010000526 DATLGF010000000
VERSION     DATLGF010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 20938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20938
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125109"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="VM406_16405"
     CDS             complement(<1..1039)
                     /locus_tag="VM406_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase N-4"
                     /protein_id="HVL77597.1"
                     /translation="MTDISLADYAAFLKQKIKMASFKGPTVDPENLHPILFPHQRDIV
                     RWAVQGGNRAIFAKFGLGKSVMQCEWLRQIIGAAGGMGLIVCPLGVRQELIRDAAMLG
                     MDVRFIRSAAEIDPAHPFYITNYETVRDGKLDPRLFTACSLDEASVLRSFGSKTYQEF
                     LPLFDGVQFKLVNTATPSPNRFKELIHYAGFLGVMDTGQALTRFFQRDSEKAGNLTLY
                     PHKEQEFWLWVSSWAVFVQYPSDLGYSDEGYVLPELEVVYHEVPTDYTKAGADRDGQA
                     LMFQDPALGLSAAATEKRDSMPARVAKVAEIVNASPDDHFILWHDLEAERHAIQEALP
                     EVVSVWGSQDLE"
     gene            complement(1036..1632)
                     /locus_tag="VM406_16410"
     CDS             complement(1036..1632)
                     /locus_tag="VM406_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HVL77598.1"
                     /translation="MTQALIVRDRSVEVSPAEREVATKVLEQAIRGVDEENHSRWRRF
                     LRHVFGMEHGEIVEVGTRIPRSGPFHRFHMAIEQAVFDAQERFSDFEQFRNWIKIGAG
                     HVDWVPGAKGGIVPLPKSISYARLEEQEMRTFHANMLAFLRGPHAAPYLWRHLGADKA
                     SEMMNTVLSGFDDSHQKQNRHQGQLAPASHQPTIRGSE"
     gene            complement(1634..1876)
                     /locus_tag="VM406_16415"
     CDS             complement(1634..1876)
                     /locus_tag="VM406_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77599.1"
                     /translation="MAQQIVTRDDIAARARAAFREGRDLSDNPFPWHSAAYVTWNEEW
                     LRLASAESAAARAHHPQLGTVPLDAAQALDLAQSGV"
     gene            complement(2006..2206)
                     /locus_tag="VM406_16420"
     CDS             complement(2006..2206)
                     /locus_tag="VM406_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003270305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77600.1"
                     /translation="MDDKKLIEDLGGPAKVAEQLGYDKAQGGVQRVHNWMTRGIPAKV
                     KLERPDLFLPGFKRSEDPEPAH"
     gene            2240..2986
                     /locus_tag="VM406_16425"
     CDS             2240..2986
                     /locus_tag="VM406_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HVL77601.1"
                     /translation="MLRENANTVLNNILFSSLKMPPMHDTMRRLYRAAEQIREVKGPA
                     ALARLLNVSAQTVHNWESRGVSRAGLLAAQERIGCNATWLESGVGDMVRATIAIESAP
                     PGRADSIEIVQYKEVMGSMGNGLVLRDQPGEIQSWRVSSEWVQKNVPHNTGVQNLCIV
                     TGFGPSMRPLFNPGDPLLVDRGVTKADRDAIYFFRVGDEGFIKHLQRVPGTGLVAISE
                     NKIYKDWVITPDMDFEVFGLVLKVWRGEDF"
     gene            2996..3472
                     /locus_tag="VM406_16430"
     CDS             2996..3472
                     /locus_tag="VM406_16430"
                     /inference="COORDINATES: protein motif:HMM:NF022214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 family protein"
                     /protein_id="HVL77602.1"
                     /translation="MHSYLSGVGGWLAFLIFTLFAGPVIGFGNLYGQMTDAVAQNPQL
                     HSYTEWAQYKLATWTVFAASAGLSVSAGYRLWKVHTAESVRFAILALWLIGPVARVLY
                     TSVGIWAFGVGVTPEDVGTVAAAGIVASLWTAYLLRSGRVRNTYGTSAAPALPANR"
     gene            3906..4244
                     /locus_tag="VM406_16435"
     CDS             3906..4244
                     /locus_tag="VM406_16435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77603.1"
                     /translation="MFTQLNPTHTAQALYDRAQGNSDVLISKRAREIAADLRKAIPTD
                     DAQAAEVGTEMLGWLDGVAELSRLTAAGDAPGLLALLKPVHDKAVAKVAEYRAIDEFE
                     ALTPTDFLPD"
     gene            4283..4420
                     /locus_tag="VM406_16440"
     CDS             4283..4420
                     /locus_tag="VM406_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77604.1"
                     /translation="MKRYRLTVKTESAQYEYSAIQKSAVEALLDAIDLFGLCAVTVRP
                     A"
     gene            4417..4629
                     /locus_tag="VM406_16445"
     CDS             4417..4629
                     /locus_tag="VM406_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77605.1"
                     /translation="MRAHSRPQRDVGDITLRDLFAGLRRWWLKRAERHYLICADVELQ
                     RMREAQMNIAYYQKRAAMARSARLSD"
     gene            4649..5467
                     /gene="bet"
                     /locus_tag="VM406_16450"
     CDS             4649..5467
                     /gene="bet"
                     /locus_tag="VM406_16450"
                     /inference="COORDINATES: protein motif:HMM:NF015776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HVL77606.1"
                     /translation="MSNLAVMPANDKTSLAMSEEDLIDVLQSSLYPGAALNSIKMVIG
                     YCKAANLDPMQKPVHIVPMWDKNAKQMRDVIMPGVGLYRTQAARSNALAGISEPEFGP
                     MVEFELDGLKVTVPEWCRVVVKRMLAGGAIAEFAAIEYWIENYATAGKDSVQPNAMWK
                     KRPRGQLAKCAQAQALRMAFPEMVGSAPTADEMEGKHLDAPEVDITPRRTEQAAPQLG
                     YCTPEKFSENQAAWRQLIVSGKKSPKDLIATLETKTRLTEDQKLTIDSWSHEND"
     gene            5482..7155
                     /locus_tag="VM406_16455"
     CDS             5482..7155
                     /locus_tag="VM406_16455"
                     /inference="COORDINATES: protein motif:HMM:NF021128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HVL77607.1"
                     /translation="MQTHTLIQGSDAWHAYRASHFNASDAPAMLGCSPYKTRSQLLHE
                     VATGTSREVDATTQRLFDDGHRFEALARPLAENIIGEELYPVVGSNGHYSASFDGLTM
                     DERIAFEHKSLNEDLRAAMQEGCTGTDLPKHYRVQMEQQCMVSGCERVLFMASRWNGD
                     ELIEERHCWYESDPDLRAELVAGWAQFEVDVAAYVAPEVQVEAVGRTPESLPALRIEV
                     TGLVTASNLEQFKSHALAVFGAINRDLQTDQDFADAEKTVKWCGDVEDRLKAAKAHAL
                     AQTESIDVLFRAIDDITAEARATRLELEKLVKARKEAVREEIRQAAVTALREHYNSIN
                     TTLGMGVVLGMPAAFGADVAAAMKGKKTITSLRDAADTTLANAKIDANANADRVRRNI
                     AILEGHKEHAFLLADAAQLAVSKDHETLGLIVKQRIADHQAAEAKRLEAERERIAAEE
                     RAKAEAKVRAEQAAREKAEREATAAAQRQQAMLEAAQRRREFESTEKKEEQPTPANVQ
                     TLPARQTRPTDAQIIEVLALHFRVHESKVLEWILDMDLDTAGQKLAASF"
     gene            7168..7617
                     /locus_tag="VM406_16460"
     CDS             7168..7617
                     /locus_tag="VM406_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77608.1"
                     /translation="MDKFKYKGKAEIKHLNVRKEGPEEDKVLAIDIKLQCTTSANMLD
                     FFHEGMKEVLFTDAGAKKNLMLKPIGFMNTVLNCDFELLGQRYGGVEVGKFQLEPKDG
                     NQVVMQFSISIQPTGDEVARISEFVMDEIDITIEPQPELDFGEKKAA"
     gene            7711..8223
                     /locus_tag="VM406_16465"
     CDS             7711..8223
                     /locus_tag="VM406_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HVL77609.1"
                     /translation="MKFEIKSRFTGTVLFSLECGSLKLCVEAAVKSGADLRGANLGDA
                     NLYGANLRGANLYGANLRGADLYGANLGDANLGDADLGGANLYGRKLSGSRPLFQLGP
                     IGSRNDYLLSFVTDQGVMIRAGCFFGTRNEFEAAINETHGDNEYGQEYRAALALIDKH
                     AELWPAIAEV"
     gene            8226..8738
                     /locus_tag="VM406_16470"
     CDS             8226..8738
                     /locus_tag="VM406_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77610.1"
                     /translation="METTLNKIRECGPCADGWEKLLRTLGKTKADDEPLSIITILDSN
                     GLDDALWCLRAVSGHDREIRLYAVWCARQVQHLMQDQRSLNALDVAERHANGQASVEE
                     LVAAGAAAWDAGAAAWDAAWAAARDAAGAARAAAWAAGDDRAAAWAAARDAQEKELRR
                     ICSKMEAVNG"
     gene            8731..9438
                     /locus_tag="VM406_16475"
     CDS             8731..9438
                     /locus_tag="VM406_16475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77611.1"
                     /translation="MDNREAVTDAEMMALFNEIRIRESSWSHSKEQSAVVLKFGQELL
                     AKAQQPSEEELAELCRGFGWRADGDKRILLDRIAHHIRDMKASIGAYRHMLEQPSETV
                     TWNAAIEAAAKVCVEQHKRFAEAIAGGAAYGSMECEKAIHSLMRPAAQEAEKPTEDHE
                     ADRWRYGVSIQRAAQEAAREQPADERKDAIPELRNIIGAHVQGDFAVHVEFRSCRAAS
                     EFAAIVSRALAEKGEHQ"
     gene            9435..9704
                     /locus_tag="VM406_16480"
     CDS             9435..9704
                     /locus_tag="VM406_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77612.1"
                     /translation="MNEDPILVCDGPNAGEIAARLRHHQALRNVQIIGAGLAAGLPID
                     MVTPVCTPLADPKPRQLTEQDRERMAAAEAKRQRRAAKRMAKEQA"
     gene            9701..11392
                     /locus_tag="VM406_16485"
     CDS             9701..11392
                     /locus_tag="VM406_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77613.1"
                     /translation="MTTTPQEALRKALEKELPSLLGPAFIRAGVRAHDGLIDQLSSAF
                     AQHLSRALAQQAAQEPQPVAWSETGALTVLNMTGDGPYGADDVAETIAFLNAAEIVRG
                     NIVSAAAPAKPADIEQAHRWATELAVSMARKFYPEAPQFEPLPDLVGVISQIDNMTTG
                     LVRAKPAENDFPAYMNALLTCKQPEVVRAARVVMAWYQGEELPVPPQISRVDADYWLD
                     NRAAILEAIERAGYRLTSNAQSFRLDPVAKPAEQEKACPTCRGNDMDAPCAYPSKGMP
                     GCLRDKRLAEQQAPATEQAPPLPEHVDVIYSPDGNGAAIEVYTVDQLRAYAEQYAAWK
                     IAALRSEQAPAFQECRHCGWECRPNTTAAKRWYPLEQAPAAQTQEQEASEAFQRGVSF
                     GANAAKVELMHLLAAQSQPSTAQEQAPAGEVPYAQKLELALAECRDAFPIPAPGSEIE
                     GWWIPAMGDPLEVPGYVKACVSTPPAVPPLEAKVIEEIRIKQGLLEWNRSRPEPVIKI
                     GMEYGVAGKVLALISAAPAAPQTKEQPKEQQESKLSKAIKAARGGDAPTNFKGYA"
     gene            11402..11791
                     /locus_tag="VM406_16490"
     CDS             11402..11791
                     /locus_tag="VM406_16490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77614.1"
                     /translation="MTKDIDLDALQAQAEVARKEADGWDEQAYPGPWQTMREQSDAIL
                     SLIARIRELESRAAPESAGAWQPKFEAIAHEQEQLAIKFCTEIATSRPDPVRLIEMAE
                     ALYLAERNDPVIGAIRADGKEGEQACG"
     gene            11782..12186
                     /locus_tag="VM406_16495"
     CDS             11782..12186
                     /locus_tag="VM406_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HVL77615.1"
                     /translation="MRLTKAQREQVRQKFGGKCAYCGCDLPERWHADHFESVQRKLDI
                     RGGRLVSTSEMHKPENDTIDNFMPSCPPCNISKHSMPLEAWRGWLAGHINSLNAYHPI
                     YRLAKAYGLIQETGAAVVFYFERAGSAPQGKE"
     gene            12189..12518
                     /locus_tag="VM406_16500"
     CDS             12189..12518
                     /locus_tag="VM406_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVL77616.1"
                     /translation="MDVHRCPACNGEPAKPTLAFFNTGENWRKHYAAEVTLPCQVCKA
                     TGMVSGWVLLRYHKGRKHKADRVERGETLFHAAKRIGVSSAELSAYEHGFADLPERAA
                     AIGAGKE"
     gene            12522..12752
                     /locus_tag="VM406_16505"
     CDS             12522..12752
                     /locus_tag="VM406_16505"
                     /inference="COORDINATES: protein motif:HMM:NF025352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HVL77617.1"
                     /translation="MSAYLSAQELADMIGCEPNSYACMRRYLQRNGWPFEPNLRGFPK
                     VSRAYHDARMAGQTAEQAAPVAGEPDFSMFSL"
     gene            12749..13846
                     /locus_tag="VM406_16510"
     CDS             12749..13846
                     /locus_tag="VM406_16510"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVL77618.1"
                     /translation="MIGRRKSPDGLPFRLYPNIGKRTTSYGYKLPDGKWAFRLSAPTT
                     DKDAVARIRAEAIERANVLNGVAPSTGPTEDLIRAYFAWQKGLPEDSEDRKAAGTLIE
                     NEREAKRLIKTFGRVKPSQIRPVHVYKYLAGRAKEEAPAKGNKEIALLSAVLEFGRRR
                     GELEDNPCRGIKYNKTRPRQKYVEAADVALMMKVARARGGSYLILALCLQVAYLTVSR
                     PDEMRALMRQAVKDEGVVVAVGKRKKGQVQRYKLIEWSPDLRAAVEEALSLQRTSSLY
                     VFGNTSGQVYSRSGFNTILSRLMGYCEKQAVEDQVTFTRFTLADMRPTAVTDRMQEGD
                     ARIIDATGHSDGRMVAKVYDRRRTRAAKATK"
     gene            complement(14172..14297)
                     /locus_tag="VM406_16515"
     CDS             complement(14172..14297)
                     /locus_tag="VM406_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77619.1"
                     /translation="MRSRDVIEGGAPPDRTRPEQCDQRSPKKDRMKRMTTIRPMM"
     gene            15086..17620
                     /locus_tag="VM406_16520"
     CDS             15086..17620
                     /locus_tag="VM406_16520"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL77620.1"
                     /translation="MTVAGANLLTGGGEVRSLMRSLDWSASPLGAPRDWPAALRMTTH
                     LMLDSGFPMFLAWGTDLCFLYNDAYIDILGRKHPAALGRPFQEIWAEIWPDISPLIAR
                     ALAGETTYAENMPLAMLRKGHMEQTWFTFSYSPLRDEAGQIRGMLCACTETTQQVLAD
                     RRQVFQLALGDRLRGLDEPEEIMAAAVEALGQELVAARVGYAEIDVPTDSIHVDRDWT
                     APPRLAIGGRVFTLTRFGPALVELLRSGTTLVIDDVQTDPRCEPYRPDYAGIGAAAAL
                     CVPLIRNGVLSAILYVHEAQPRHWSGHDIRLVQDVADRIWSMVERARAEERRRRAEAA
                     LHAQLEAERNRLRSLFEQAPSFMAVLRGPNHVFELANAAYLRLIGTRDLIGKPARQVL
                     PDVEGQPFFDLLDRVYRTGEPYFENEARLMLQPVQGEPQIERFIDFVYQPLTDASGNV
                     SGIFVDGYDVTERVHAFAALREADQRKDEFLAMLAHELRNPLAPISAAAQLLRISGND
                     PDRVRKTSEVIARQADHMTGLVNDLLDVSRVTRGMVKLDFQVQDLRDVVRAALEQTRA
                     LLEAKGHAVALDFGADCLHAPQVCGDRVRLVQIVSNLLNNAAKYTPDGGRITLRLQVS
                     AGEASLTVSDTGIGIHAELLPHIFEPFTQAERTPDRSQGGLGLGLALVRRLAELHGGS
                     VSASSPGPNAGSSFTLRLPRVRGTRVVKDAAGPAGAPARRVVPLRIMVVDDNEDAAHS
                     LAMLLGAEGHQVSTAARADAALALAREQAPQVCLLDIGLPGMDGYELARRLRALSQTR
                     DAMLIALTGYGQPQDRARSFEAGFDHHLVKPAEIRRLLDLVSRRAA"
     gene            18032..18430
                     /locus_tag="VM406_16525"
     CDS             18032..18430
                     /locus_tag="VM406_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HVL77621.1"
                     /translation="MENTNYTTSQTTGNGSAATRDAANKLYSDAKGAAQRTGETLRSE
                     LSVLKQDLDDLMSRSSSMSDEELSRAHAQLMAKFSSLRYAARGFADEATRQWTRGMET
                     TNQYVKEKPMQSVGLAVGTGLLLGLLFSRR"
     gene            18493..18861
                     /locus_tag="VM406_16530"
     CDS             18493..18861
                     /locus_tag="VM406_16530"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HVL77622.1"
                     /translation="MFTALHKSKQLYLITLDRVTDYIDLLKVEMKIREYQLAFRMAGF
                     AVALLFGLLATIFLGLAIIISFWDTAFRALAAWGVVILYGGVAGVSLYYCVKHFRSPP
                     LGGMLRNELQRDINVIKENL"
     gene            18858..19355
                     /locus_tag="VM406_16535"
     CDS             18858..19355
                     /locus_tag="VM406_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77623.1"
                     /translation="MSTVQSLEQERQAILDRMQMRRETYRRMLADGEDPRMLAETHEQ
                     YRQGHGDGRAGESAANRAAHADPRSMKRFAAAGVGDDSTVYTYRRVNDSFPRTRVMQV
                     VAEHPVLCALGAAAVLAIGPRRIMRTVATSSAAIGAVAQSPTAMDMIGRVLTLAGAVA
                     QRRNG"
     gene            19497..19652
                     /locus_tag="VM406_16540"
     CDS             19497..19652
                     /locus_tag="VM406_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HVL77624.1"
                     /translation="MKKIISVLSVLVMSIGVSACNTMAGMGQDIERGGERMQGAAERQ
                     QQEMQRR"
     gene            19715..20002
                     /locus_tag="VM406_16545"
     CDS             19715..20002
                     /locus_tag="VM406_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL77625.1"
                     /translation="MKERSAHVSYLQGRRWRCWRRWRHADDEFPGVVERMPEGKTGNW
                     IIGGRKVVVRQRTELDEDRGPFRVGACVEVEYDDREVEELETKQPERCQGR"
     gene            complement(20017..>20938)
                     /locus_tag="VM406_16550"
     CDS             complement(20017..>20938)
                     /locus_tag="VM406_16550"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HVL77626.1"
                     /translation="FGGVLFAVLLRAPANWLTEHASLGEGAALGLSVVAILGGLIGML
                     YLFAFPLAEQVGSLIETLPQAMARLRAWLGDYRWARPLQPLVVELSHIRLDTLTLGRA
                     TWILSSTAAALGGALVVIFIGIYLAAQPRLYQRGIMHLLPRRRRPRAYEVMDEIGVAL
                     RWWLVGRLATMTLVGVAAGTGLWLLGVPYAFTLGVLTGVLEFIPYVGPILGAAVPVLI
                     AFNIDPELGLYVVLLYIGIQALEGYLLTPLIEQRTVSLPPALVIFATILLAALTGPLG
                     VVLASPLTASLIVAVRLLYVEDVVEQPLKT"
BASE COUNT         4240 a         6608 c         6625 g         3465 t
ORIGIN      
        1 cttccaagtc ttgcgagccc cacacgctca ccacttccgg cagcgcctcc tggatcgcat
       61 ggcgctcggc ttccaggtcg tgccagagaa tgaagtgatc gtccggtgag gcgttgacga
      121 tctcggccac ctttgccacg cgcgccggca tcgagtcgcg cttctcggtg gccgctgccg
      181 acaggcccag agccgggtct tgaaacatca gcgcctggcc gtctcggtcg gcgccggcct
      241 tcgtgtagtc ggtcggcacc tcgtgataga cgacttccag ctcgggcagt acatagccct
      301 cgtcggaata gccgaggtcg ctcgggtact gcacgaacac cgcccaggaa ctcacccaca
      361 gccagaactc ctgttctttg tgcgggtaca gggtcaggtt gccggccttc tcggaatcgc
      421 gctggaagaa ccgggtcaag gcctggccgg tgtccatcac gcccaggaag ccggcgtagt
      481 ggatcagttc cttgaaacgg ttcgggctgg gcgtggcggt gttgaccagc ttgaactgca
      541 cgccgtcgaa cagcggcaag aactcctgat aggtcttgga gccgaacgag cgcagcacgc
      601 tcgcctcatc caggctgcag gcggtgaaca ggcgcggatc gagcttgccg tcgcgcaccg
      661 tctcatagtt ggtgatgtaa aagggatggg ccgggtcgat ctcggcggcc gagcggatga
      721 agcgcacgtc catgcccagc atggccgcgt cgcggatcag ttcttgccgc acgccgagcg
      781 ggcagacgat caagcccatg ccgccggccg cgccgatgat ctggcgcagc cactcgcact
      841 gcatcaccga cttgcccagg ccgaacttgg cgaagatcgc ccggttcccg ccctggactg
      901 cccagcgcac gatgtcgcgc tggtgcggga acaggatcgg gtgcaggttt tcaggatcga
      961 cggtcggccc cttgaagctg gccatcttga tcttctgctt caggaacgcg gcgtaatccg
     1021 ctagactgat gtcagtcatt cactacctct gatagtgggt tgatgagaag ccggcgccag
     1081 ttgcccctgg tgccggtttt gtttttggtg ggagtcgtcg aagccggaga gaacggtatt
     1141 catcatctcg gaggctttgt ccgcgccgag gtggcgccac aggtagggcg cggcatgcgg
     1201 cccgcgcagg aaggccagca tgttggcgtg gaaggtgcgc atctcctgct cttccaggcg
     1261 cgcgtagctg atcgacttgg gcagcgggac gatgccgccc ttggcgcccg gcacccagtc
     1321 cacatggccg gcgccgatct tgatccagtt gcggaactgc tcgaagtcgg aaaagcgctc
     1381 ctgcgcatcg aacaccgcct gctcgatcgc catgtgaaag cggtgaaagg ggccggagcg
     1441 gggaatgcgc gtgccgacct cgacaatctc gccgtgctcc atgccgaaca catggcgcag
     1501 gaagcggcgc cagcgggaat ggttctcttc atcgaccccg cgaatcgcct gttcgagcac
     1561 cttggtggcg acctcgcgct cggccggcga gacctcgacc gagcggtcgc gcacgatcag
     1621 ggcttgcgtc atctcaaacc cccgactgcg cgagatcaag cgcctgcgca gcatcaagcg
     1681 gcacagtgcc aagctgggga tggtgtgcgc gcgcggcggc cgattcggcg ctagccaggc
     1741 gcaaccactc ttcgttccat gtcacatagg cggccgagtg ccaggggaac gggttatccg
     1801 acagatcccg cccttcccgg aacgctgcgc gcgcccgcgc ggcgatgtca tctcgcgtca
     1861 cgatctgctg agccatccct tcccccctgt tgttgattct ttgcaatgct tttgtttccg
     1921 tcgcgaatat gcttgcgggc cggaaaccca cttctggccg aacgaatcca gccgcccatt
     1981 cctgggaggc tggatcaacc tgcgcttagt gcgcgggctc cggatcttcc gatcgcttaa
     2041 agccgggcag gaagaggtct ggccgttcca acttgacctt ggcggggatg ccgcgcgtca
     2101 tccagttatg gacgcgctgc acaccgccct gcgccttgtc gtagcccagt tgctcggcga
     2161 ccttggccgg cccgcccagg tcttcgatca gttttttgtc atccatagtc ttggttccct
     2221 tcaacaccaa ttaaacacca tgttgagaga aaacgcaaac accgtgttga acaacatttt
     2281 gtttagctca ctcaagatgc cgcccatgca tgacacgatg aggcggctgt accgagctgc
     2341 agaacaaata agggaagtaa aggggccggc ggcgcttgct cgcctgctga acgtctctgc
     2401 gcaaaccgtc cataactggg agagccgcgg cgtctcgcgc gccgggctgt tggccgctca
     2461 agagcgcatt ggctgcaatg cgacttggct ggagagtggc gttggcgata tggtccgagc
     2521 cacgatcgct attgagtccg ccccgcccgg gcgcgcggat tcaatcgaga tagttcagta
     2581 caaggaagta atgggatcga tgggaaacgg cctggtcctg cgcgatcagc caggcgaaat
     2641 tcagagttgg cgcgtgtcgt cggaatgggt gcagaagaac gtcccgcaca acactggtgt
     2701 gcagaacctg tgcattgtca cggggttcgg cccgtcgatg cggccactat tcaatcccgg
     2761 cgatccgcta ctggttgacc gcggcgtcac caaggcggac cgggacgcga tctatttctt
     2821 ccgcgtcggc gacgaggggt tcatcaagca ccttcagcgc gtgccgggca ccggcctagt
     2881 agcaatatcc gagaacaaga tttacaagga ttgggtcatc acgccggaca tggatttcga
     2941 agtgtttggg ctcgtgttga aggtgtggcg cggcgaagat ttctaagggg ggaacatgca
     3001 ttcatatctt tccggcgtag gtggctggct tgcgtttttg atattcacgt tgtttgccgg
     3061 cccagtcatt ggcttcggga acctgtatgg acaaatgaca gatgccgtcg cacagaatcc
     3121 gcagcttcat agctacaccg aatgggctca atacaaattg gcgacatgga cggtgtttgc
     3181 cgcatcggct ggccttagcg tttccgccgg ctatcgactc tggaaggtcc acacggcgga
     3241 aagcgtccgg tttgccatcc ttgccctgtg gcttattggg cccgtggcgc gggttctcta
     3301 taccagcgtc ggcatttggg catttggagt cggggtaacg ccagaagatg tcggcactgt
     3361 agcagcggcc ggaatcgtcg cgtctctttg gaccgcatat cttctgcgat ctggccgagt
     3421 gcggaacaca tatggcacca gcgcagcgcc ggcattgcct gctaatcgct agcgccatcg
     3481 cgtggtctgc tgcattcccg ctctcgccac caggtgatcg gcatcgccga cagcgggaaa
     3541 caccaatgac gcaatcggcc ttatcacgcc ttcagcccgg cgaactctgc atcgtggttc
     3601 tgcaagacgg gacgcagcgc gaaggcgctt ggtcaccgga tgatcggagc ttcactttta
     3661 cagacggcaa gggctccggc accgtcccgg cgtgcgatgt ctacgaatgg tggccggcaa
     3721 gcgtccggtt ctgattgatc tccgcccatc cccacccgct ccggcgggtt ttttttcgcc
     3781 ctccgcatct cactacggta cgcataccgt agttgttccg tcgcggaatt aaacatagtg
     3841 tttgcaattc gtttaaacgt ggtgtttaat acgcacatca acaccacgaa ctcggaagca
     3901 acaccatgtt cacccagctc aaccccaccc acacagcgca agccctctac gaccgcgccc
     3961 aaggcaacag cgatgtgctg atttccaagc gtgcgcgcga gattgccgct gatcttcgca
     4021 aggcgatccc gaccgacgac gcgcaagccg cggaagtggg cacggaaatg ctcggctggc
     4081 tggacggcgt ggccgaactc agccgcctga ctgccgcagg cgatgccccc ggcctgctcg
     4141 ccctgctcaa gccggtgcac gacaaggcgg tcgccaaggt cgccgaatac cgtgcgatcg
     4201 acgaattcga ggccttgacc ccgaccgatt tcctgccgga ctgaccggcc cttttttcaa
     4261 gctagcacag ggagccagaa caatgaagcg ttatcgcctg accgtcaaaa ccgaatccgc
     4321 gcagtacgag tacagcgcca tccagaaaag cgctgtcgag gcgctgctcg atgccatcga
     4381 tctgttcggt ctgtgcgccg tgaccgtgag gccggcatga gggcccattc caggccgcag
     4441 cgcgacgtgg gcgacatcac cctgcgcgac ctgtttgccg gcctgcgccg ctggtggctc
     4501 aagcgcgccg aacgccacta cctgatctgt gcggacgttg aactgcagcg catgcgtgaa
     4561 gcgcagatga acatcgcgta ttaccagaaa cgcgcagcca tggcccgctc ggcccggctg
     4621 tcggattgaa cacaaaccgg agaaccgcat gagcaacctg gccgtcatgc ccgccaatga
     4681 caaaacctcc ctcgcaatga gcgaggaaga cctgatcgac gtgctgcagt cgagcctgta
     4741 tcccggtgcc gcactcaaca gcatcaagat ggtgatcggc tactgcaagg ctgccaatct
     4801 cgatccgatg caaaagcctg ttcacatcgt gccgatgtgg gacaagaacg ccaagcagat
     4861 gcgcgacgtg atcatgccgg gcgtaggtct ctaccgcaca caggccgcgc gcagcaatgc
     4921 gctggccggc atcagcgagc cggagttcgg cccgatggtc gagttcgaac tggatggcct
     4981 gaaggtcacg gttccggaat ggtgccgggt cgtggtcaag cgcatgctcg ccggcggcgc
     5041 gatcgccgag ttcgcggcca tcgaatattg gatcgagaac tacgccacgg ccggcaagga
     5101 cagcgtgcag cccaatgcca tgtggaagaa gcgcccgcgc ggccagctcg ccaagtgcgc
     5161 ccaagcccag gcactgcgca tggccttccc ggagatggtg ggttcagccc ccactgcgga
     5221 cgaaatggaa ggcaagcacc tagacgcgcc tgaagtcgac atcacgcccc gccgcaccga
     5281 acaggccgcc ccccagcttg gctactgcac gccggagaag ttctccgaga accaagccgc
     5341 atggcgccaa ctgatcgtca gcggcaagaa gtcgcccaag gacctgatcg ccacgctgga
     5401 aaccaagacc cgattgaccg aagaccagaa gctgaccatc gacagctggt cccacgaaaa
     5461 cgattgaagg gagaaccgac catgcaaacc cataccctga ttcaaggcag cgatgcctgg
     5521 catgcctacc gcgccagcca cttcaatgcc agcgacgccc cggccatgtt gggctgctca
     5581 ccctacaaga ctcgaagcca actgctgcac gaggttgcaa ccggcaccag ccgagaagtc
     5641 gacgcgacca cgcagcgcct gttcgatgac ggccaccgct tcgaagcttt ggcgcgcccg
     5701 ctggccgaga acatcatcgg cgaagagctt tatccggtag ttggctccaa cgggcactac
     5761 agcgcatctt tcgacggcct gaccatggac gagcgcatcg cgttcgagca caagagcctg
     5821 aacgaagacc tgcgcgcggc gatgcaggag ggctgcaccg gcaccgacct gccgaagcac
     5881 tatcgcgtcc agatggaaca gcagtgcatg gtctccggct gcgagcgcgt gcttttcatg
     5941 gcgtcccggt ggaacggaga tgaactgatc gaggaacgcc actgctggta cgaatcggat
     6001 cctgatctgc gggcagaact ggttgccggc tgggcccagt tcgaagtgga tgtcgccgcc
     6061 tacgtcgcgc ccgaagtgca ggtcgaagcg gtcggccgca ctcccgaatc cctgcccgcc
     6121 ctgcgcatcg aggtcaccgg cctggttacc gcctccaacc tcgagcagtt caagtcgcat
     6181 gcgctggcgg tgttcggcgc gatcaatcgc gatctgcaga ccgaccagga cttcgccgat
     6241 gccgagaaga ccgtcaagtg gtgcggcgac gtggaagacc gcctgaaggc cgccaaggct
     6301 cacgcgctgg cgcagaccga aagcatcgac gtgctgttcc gcgcgatcga cgacatcacc
     6361 gccgaggcgc gcgcgacccg cctggaactt gaaaagctgg tcaaggcccg caaggaagcg
     6421 gtgcgcgaag agatccggca agctgcggtg accgcactgc gcgagcacta caacagcatc
     6481 aacaccactc tcgggatggg tgtggtgctg ggcatgccgg ccgccttcgg tgccgatgtt
     6541 gccgcagcca tgaagggcaa gaagacgatc accagcttgc gcgatgccgc agacaccacg
     6601 ctggcgaacg ccaagatcga cgccaatgcg aatgccgacc gcgtgcgccg caacatcgca
     6661 atcctcgaag gccacaagga acacgccttc ctgctcgccg atgcagccca actggccgtc
     6721 agcaaggacc atgaaacgct gggcctgatc gtcaagcagc gcatcgccga tcaccaggcc
     6781 gcagaggcca agcgcctgga agccgaacgc gagcgcatcg ccgcagagga acgcgccaag
     6841 gccgaggcca aggttcgcgc cgagcaggcg gcgcgcgaga aggccgaacg ggaggccacg
     6901 gccgctgccc agcgccagca ggccatgctc gaagcagcgc agcgccgccg cgagttcgag
     6961 tccactgaga agaaggaaga gcagccgaca cccgccaacg tgcagacgct gcccgcgcgc
     7021 cagacccgcc ccaccgacgc ccagatcatc gaagtccttg ccctgcactt ccgcgtgcac
     7081 gagtcgaagg tccttgaatg gattctcgat atggatctcg ataccgctgg ccagaagctg
     7141 gccgcttcgt tctgatcagg ctcagacatg gacaagttca aatacaaggg caaggccgag
     7201 atcaagcatc tgaacgtgcg caaggaaggc ccggaagaag acaaggtgtt ggccatcgac
     7261 atcaagctcc agtgcacgac aagcgccaac atgctcgatt tcttccatga gggcatgaag
     7321 gaagtgctgt tcacggacgc cggcgcaaag aagaacctga tgctcaagcc gattggcttc
     7381 atgaacaccg tcctgaactg cgatttcgag ctgctgggcc agcgttacgg cggggtcgag
     7441 gtcggcaagt tccagctgga gccgaaggac ggaaaccagg tggtgatgca gttctcaatc
     7501 agcatccagc ccacaggtga cgaggtggcg cgcatctcgg aattcgtgat ggacgagatc
     7561 gacatcacga tcgaaccaca gccagaactg gatttcggcg agaagaaggc cgcatgacat
     7621 gtctcctgct tcttgctgtc gtgatcgcgg cagtgctggc aagcgccagc gacaactgga
     7681 acgacgaaca tcattgaggg agaagaacaa atgaaattcg aaatcaaatc gcgcttcact
     7741 ggcacagttc tgttttcgct tgaatgcgga agcctcaagc tgtgcgtgga ggcggcagta
     7801 aaaagcggcg cggacctgcg cggcgcgaac ctgggcgacg cgaacctgta cggcgcgaac
     7861 ctgcgcggcg cgaacctgta cggcgcgaac ctgcgcggcg cggacctgta cggcgcgaac
     7921 ctgggcgacg cgaacctggg cgacgcggac ctgggcggcg cgaacctgta cggaagaaaa
     7981 ctttctggtt ctcgcccgct ttttcagctc ggcccaatcg gatcgcgcaa tgactacctg
     8041 ctgtccttcg tcaccgatca gggtgtgatg atccgcgcag gctgcttctt cggcacgcgg
     8101 aacgagtttg aggcagctat caatgaaacg cacggcgata acgaatacgg gcaagagtac
     8161 cgcgctgcgc tggcactaat cgacaagcat gcagagttgt ggcctgctat agcggaggta
     8221 taagtatgga aaccacacta aacaaaatcc gcgagtgcgg tccgtgcgcg gacggctggg
     8281 aaaagctgtt gcgcacactc ggcaaaacga aggcagacga cgagccgctg tcgatcatta
     8341 ctatcctcga cagcaatggg ttggacgatg cgctgtggtg cctgcgtgca gtgagcgggc
     8401 atgaccggga aatccgactg tatgcagtct ggtgcgcgcg gcaagtgcag catctgatgc
     8461 aggaccagcg cagcttgaac gcgctcgatg ttgcagagcg acatgcgaat ggacaggcgt
     8521 cagtcgagga actggtcgcc gccggggccg ccgcctggga cgccggggcc gccgcctggg
     8581 acgccgcctg ggccgccgcc agggacgccg ccggggccgc cagggccgcc gcctgggccg
     8641 ccggggacga cagggccgcc gcctgggccg ccgccaggga cgctcaggaa aaagagctta
     8701 ggcgcatctg ctcgaaaatg gaggcggtga atggataaca gagaagcggt aacggatgcc
     8761 gaaatgatgg cactgttcaa cgagatacgg atcagggaat cctcttggtc ccactcgaaa
     8821 gagcagagcg cagtcgtgct caagttcggg caagaactgc tcgccaaagc gcagcagccg
     8881 agcgaagaag aactggcaga actgtgccga ggcttcggct ggcgggcgga tggcgataag
     8941 cgaattctgc ttgaccgcat tgcgcaccac atccgcgaca tgaaagccag catcggcgca
     9001 taccggcaca tgctggagca gccgagcgag acggtcacat ggaacgcggc gatagaagcg
     9061 gcggcgaagg tgtgcgtgga gcagcacaag agatttgctg aagcaatcgc tggtggcgct
     9121 gcttacggct cgatggagtg cgagaaggcc atccactctc tcatgcgccc cgccgcgcag
     9181 gaagccgaga agccgaccga ggaccacgaa gctgaccgct ggcgatatgg ggtaagcatt
     9241 caacgcgccg cgcaggaagc cgcccgagag cagccagccg acgagcggaa ggatgcgatc
     9301 cccgagctgc gcaacatcat cggcgcgcat gtgcagggag atttcgcggt gcatgtcgag
     9361 ttccgatcct gccgcgccgc atctgaattt gccgcaatag tcagccgcgc ccttgcagag
     9421 aaaggagagc accagtgaat gaagatccga ttctggtctg cgatggcccg aacgcaggag
     9481 aaatcgccgc ccgcctccga caccatcaag cgctgcgcaa cgtgcagatc atcggcgctg
     9541 ggctggctgc aggcctgccg atcgacatgg tgacgccggt atgcactccg ctggccgatc
     9601 cgaagccgcg ccaacttacc gaacaggacc gcgagcgcat ggccgctgcg gaagcgaaac
     9661 ggcagcgccg cgctgcgaaa cgcatggcga aggagcaggc atgaccacca caccacaaga
     9721 agcactgcgc aaggcgctgg aaaaagaact gccaagcttg cttgggccgg catttatcag
     9781 ggctggagta cgggctcatg atggactcat tgaccagctt tcgtccgcat tcgcccagca
     9841 cctgagcaga gcgcttgcac agcaggcagc gcaggagccg cagccggtgg cgtggagcga
     9901 aacgggagcg ctgaccgtgc tcaacatgac cggagatggc ccttacggcg ctgatgacgt
     9961 tgccgagacc atcgcattcc tgaacgctgc cgagatagtg cgcgggaaca ttgtcagcgc
    10021 tgccgcccct gccaagcctg ccgacataga gcaggcgcac cgatgggcga ccgagcttgc
    10081 cgtctcgatg gcgcgcaagt tctacccgga agccccgcag ttcgagccgt tgcctgatct
    10141 ggtcggcgtg atttcgcaga tcgacaacat gacgaccggg cttgtgcgtg ccaagcctgc
    10201 cgagaacgac tttccggcct acatgaatgc gctgctgacc tgtaagcagc ccgaagtagt
    10261 cagagccgct cgcgtcgtca tggcgtggta tcagggcgaa gaattgcctg tgccgcctca
    10321 aatcagccgc gtcgatgcgg attactggct ggacaaccgc gccgcgatcc tcgaagccat
    10381 cgagcgcgcc gggtatcgtc tgacaagcaa tgcgcagagt ttccggcttg atcctgttgc
    10441 caagcctgcc gagcaggaga aggcatgtcc gacatgccga ggcaatgaca tggacgcacc
    10501 atgcgcctat ccaagcaagg gaatgcccgg ctgcctgcgg gataagcgct tggccgagca
    10561 gcaagcgcca gctactgagc aagcgccgcc gctgccggaa cacgtagatg tcatctattc
    10621 tccggacggc aacggtgcgg caattgaggt ctacaccgtc gaccaactcc gcgcctacgc
    10681 cgagcaatac gccgcatgga agattgccgc cctccggtct gagcaagcgc cagccttcca
    10741 agagtgccgc cattgcggct gggagtgcag acccaacacg acagcagcaa agcgctggta
    10801 tccgcttgag caagcgccag cagcgcagac acaggagcag gaggcaagcg aagcgtttca
    10861 gcgtggcgtc tcattcggcg cgaacgcggc aaaggtggag ctgatgcact tgctcgccgc
    10921 tcagtctcag ccatccacgg cgcaagagca agcgccagcg ggagaagtgc cgtatgcgca
    10981 gaagctagaa cttgcgctgg ccgagtgccg cgacgccttc ccgatccccg cgcccggatc
    11041 agagattgag ggatggtgga ttcctgcgat gggcgacccg cttgaggtgc caggctacgt
    11101 caaggcgtgc gtctccaccc cgccagcagt gccgcccctt gaggcaaagg tcatagagga
    11161 aatccggatc aagcaaggtt tgctggagtg gaaccgctca cggcccgagc cggtaatcaa
    11221 gatcggcatg gaatacggcg tcgccgggaa ggtgctcgcg cttatctctg ccgctcctgc
    11281 agcaccacag accaaggagc agccgaagga gcagcaggaa tcgaagctct ccaaggcgat
    11341 caaggcagca cgaggcggtg atgctcctac gaacttcaaa ggctacgcgt aaggaaaccc
    11401 catgaccaaa gacatcgatc tggacgcatt gcaggcgcaa gccgaagtag cgcgtaagga
    11461 agccgatgga tgggacgagc aagcctatcc cggaccgtgg cagacaatgc gcgagcagtc
    11521 ggacgccatc ctctccctga ttgcccgcat ccgtgagttg gagagtaggg ccgcaccaga
    11581 gagcgcgggg gcgtggcagc cgaagttcga ggccatcgcg cacgaacaag agcaactcgc
    11641 catcaagttc tgcaccgaga ttgccacaag ccggcccgac cccgtgcgac tgatcgaaat
    11701 ggccgaagcg ctgtatctgg ccgagcggaa cgaccctgtg atcggggcta tccgggcaga
    11761 tgggaaggaa ggagaacagg cgtgcggttg accaaagcac agcgcgaaca ggtcaggcag
    11821 aagttcggcg gcaagtgcgc atactgcggc tgcgatcttc cggagcgctg gcatgcggat
    11881 cattttgagt cggtgcagcg caagctggac atacgtggcg ggcgtcttgt ttcgaccagc
    11941 gaaatgcaca agccggagaa cgacaccatc gacaacttca tgccgtcttg cccgccgtgc
    12001 aatatcagca agcactcgat gccgcttgaa gcatggcgcg gatggctggc cgggcacatc
    12061 aattcgctca atgcctacca cccgatctac aggcttgcaa aggcatacgg gctgattcag
    12121 gaaaccgggg ccgctgtcgt gttctatttc gagcgcgccg ggtccgctcc ccaagggaag
    12181 gagtaagcat ggacgtacat cgttgtccag cctgtaatgg cgagccggcg aagccgacac
    12241 tggcgttctt caatacgggc gagaactggc gcaagcacta cgcggcagaa gtgaccttgc
    12301 catgtcaggt gtgcaaagcc actggcatgg tcagcggctg ggtgctgctg cgctaccaca
    12361 agggccgcaa gcacaaggct gaccgcgtag agcgcggcga gacattgttc catgccgcca
    12421 agcgcatcgg cgtgtcgtcc gcagaattga gcgcatacga gcacggcttc gcagatttac
    12481 cggagcgcgc tgcggctatc ggtgcaggga aggagtaagc agtgagcgca tatctttctg
    12541 cgcaggaact ggccgacatg atcggctgcg aaccgaacag ctatgcctgc atgcgccgct
    12601 acctgcagcg caacggctgg ccgtttgaac cgaacctgcg cggcttcccc aaggtctcgc
    12661 gcgcgtatca tgacgccagg atggcagggc agacagccga gcaggccgcg ccagtcgcgg
    12721 gcgaacctga tttttcgatg ttctccctat gattggtcgc cgcaaatccc cggacggctt
    12781 gccgttccgc ctgtacccga acatcggcaa gcgcaccacc agctacggct acaagctgcc
    12841 ggacgggaaa tgggccttcc gtctttctgc tccgaccacg gacaaggacg cggtggcacg
    12901 catccgcgcc gaggcgatcg agcgcgccaa cgtcctgaac ggcgtggcgc cgagtaccgg
    12961 gccgaccgaa gacctgatcc gcgcctactt cgcctggcag aaggggctgc cggaagacag
    13021 cgaagaccgc aaggcggccg ggaccctgat cgagaacgag cgcgaggcca agcgcctgat
    13081 caagaccttc ggccgcgtga agccgtcgca gatccgcccg gttcacgtct acaagtacct
    13141 ggccggccgc gccaaggaag aagccccggc caagggaaac aaggagatcg cgctgctgtc
    13201 agccgtgctg gaattcggcc ggcgccgcgg cgagctcgaa gacaatccgt gccgcggcat
    13261 caagtacaac aagacccgcc cgcgccagaa gtacgtggaa gcggctgacg tggcgctgat
    13321 gatgaaagtc gcccgcgcgc gcgggggctc ctacctgatc ctggcactgt gcctgcaggt
    13381 ggcttatctg accgtgagcc ggccggacga gatgcgcgcg ctgatgcgcc aggcggtcaa
    13441 ggatgaaggg gtagtcgtcg ccgtcggcaa acgcaagaag ggacaggttc agcgttacaa
    13501 gctgatcgag tggtcgccgg acctgcgcgc cgccgtggaa gaagccctga gcctgcagcg
    13561 cacgtccagc ctgtacgtgt tcggcaacac ctccggccag gtctacagca gaagcggctt
    13621 caacaccata ctgagccgct tgatgggcta ctgcgaaaag caggctgtcg aggatcaggt
    13681 cacgttcacc cgctttaccc tggccgacat gcgccccact gcggtgaccg accgcatgca
    13741 ggaaggcgat gccaggatca tcgatgccac cggccacagc gacgggcgga tggtggcgaa
    13801 ggtgtacgac cggcgccgca cgcgcgcggc caaggcgacg aaatgaccgg taaaatcatt
    13861 ggaagatccc gaaacacccc gcgcattcat gcgggtttcg gcgcgcatca tcttccaaaa
    13921 ttcaagcgtg ctctgccaat ggaatcagag acttacggcg gtttgcgctc aggattgtga
    13981 ttcctgtcgt cgtgggttcg agtcccatcg gccaccccac cggatacaaa aaaggcccag
    14041 tcttcagact gggccttttg ctttttgaca gcgccacgcc ggatgagaaa taacaaacct
    14101 atgaacttgg atcatggaga aatgccgaag aaataaccat ccatttcgag gcatcttatg
    14161 aactccatca tctacatcat cggcctgatc gtcgtcatcc tcttcatcct gtccttcttc
    14221 ggactgcgct gatcgcactg ctcggggcgc gtgcggtcag gcggtgcgcc gccttcgatc
    14281 acatcgcgcg atcgcacgtg tctctcatgg cgaacaaagc gtgctgagga agacccgatt
    14341 gccggatcgc cgaggtcgac ggattcgtcg gccgaagcgc caaggagagg tctgaatctc
    14401 agatcgtgca cgcgcgccgc gcataccgtg ctcgtgtgct ttgcctctcg gttccgttct
    14461 tcggaccggt acgtgccctc ttcgtgagcg gccatccgcg gcgtgatgag agcgctcccg
    14521 gctttcgatc gcgcccgctc gcacgaggcg cagtgttgcc acgagttaca tcgagcccca
    14581 cgtacagtta cccacggaat gcgtgggcaa gtctgtggac aagtcgtgga ctgtttccca
    14641 agtccttgat ccgaaaggaa ccggcgccgt tgatcaaaat caacgcaagg cgcgggatgc
    14701 aagcccccgg acggattgcc aacgcggcac taacccggat ggtcgatgtc ttccaattgc
    14761 agaagcacgc tgacgaacac gctgacgatc agcgcggcgg tgatcactcc cagaactgat
    14821 atcacggcgg ttaccatggc ggcctccttg ctggctgtcg aacgtcatta cagcactggc
    14881 tccggagaag actcttgcgt tacgtcacca tctcccgggc aagttgacga gcgtcaacaa
    14941 tgccacgctc tcttgcttgc atgtttatac ttgccgccta gttccacgcg gccatcccgc
    15001 tccttgcctg cgcaagccgc ctgaccaacc catccgagga aagatgcagg gccgcccttc
    15061 cccttccgct cccaccgagg gaaccatgac cgtcgccggg gcgaacctgc tgaccggcgg
    15121 cggcgaggtg cgctcgctga tgcgctcgct cgactggtca gcatcgccgc ttggcgcgcc
    15181 gcgcgactgg ccggccgcgt tgcgcatgac cacccacctg atgctcgatt cgggcttccc
    15241 gatgtttctg gcatggggaa cggacctgtg ctttctctac aacgacgcct acatcgatat
    15301 ccttggccgc aagcacccgg ccgcgctggg gcggcctttc caggaaatat gggcggaaat
    15361 ctggccggat atcagcccgc tcatcgctcg cgcgctggcc ggcgagacga cctacgccga
    15421 gaacatgccg ctcgccatgc tgcgcaaggg gcacatggaa cagacgtggt ttacgttctc
    15481 gtattcgccg ctgcgcgacg aggcgggaca aatcaggggc atgctctgcg cgtgcaccga
    15541 aaccacgcag caggtgctgg ccgatcgccg tcaggtattc cagctcgcac tgggcgaccg
    15601 cctgcgcgga ctcgacgagc cggaagagat catggcggcg gcggtcgagg ccctggggca
    15661 ggagctggtg gccgccaggg tcggctatgc cgaaatcgac gtcccgaccg acagcatcca
    15721 cgtcgaccgt gactggacgg ccccgccgag gctggccatc ggcgggaggg tcttcacgct
    15781 cacccgtttc gggcctgcgc tcgtcgagct gctgcgcagt ggtaccacgc tggtcatcga
    15841 cgacgtgcaa acggatccgc gctgcgagcc gtaccggccg gattacgcgg gcatcggcgc
    15901 agcggcggcg ctgtgcgtgc cgctgatccg caatggcgtg ctgagcgcca ttctgtatgt
    15961 gcatgaggcg cagccgcggc actggagcgg gcacgatatc cggctggttc aggacgtggc
    16021 cgaccgcatc tggtccatgg tcgaacgcgc gcgcgccgag gagcggcgcc ggcgcgccga
    16081 ggccgcgctg cacgcgcagc tggaggcaga gcgcaatcgt ttgcgcagcc tgttcgagca
    16141 ggcacccagc ttcatggcgg tgctgcgcgg acccaatcac gtattcgaac tggccaacgc
    16201 cgcctatctg cgcctgatcg gcactcgcga tctgatcggc aagccggcac ggcaagtcct
    16261 gccggacgtg gaaggccaac cgttcttcga tctgcttgac cgcgtatacc gaaccggcga
    16321 gccctacttc gaaaatgagg cgcgcctgat gcttcagcct gtgcaaggcg aaccgcagat
    16381 cgagcgcttc atcgatttcg tgtaccagcc gctgaccgat gcaagcggca acgtgtcagg
    16441 catcttcgtc gacggctacg acgtgaccga gcgcgtgcac gcgttcgccg ccctgcgcga
    16501 ggcggatcag cgcaaggacg agttcctcgc aatgctcgcg cacgagctgc gcaatccgct
    16561 ggcgccgatc agcgctgcag cgcaactgct gcgcatctcc ggcaacgacc cggatcgcgt
    16621 gcgcaagacc agcgaagtca ttgcgcgcca ggccgatcac atgacagggc tcgtcaacga
    16681 tctgctcgac gtgtcgcgcg tgacgcgcgg catggtcaag ctcgatttcc aggtgcagga
    16741 tctgcgcgac gtggtgcgcg cggcactcga gcagacccgc gccttgctcg aggcgaaggg
    16801 ccatgcggtc gcgctggact tcggcgctga ctgcctgcac gcgccccagg tgtgcgggga
    16861 ccgcgtgcgg ctcgtccaga tcgtcagcaa tctgctcaac aatgctgcga agtacacgcc
    16921 ggacggcggg cgcattacac tccgcctgca agtttcggcc ggtgaagcga gcctgaccgt
    16981 cagtgatacc ggcatcggca tccatgccga actgctgccg cacatcttcg aaccgttcac
    17041 gcaggccgag cgcacgccgg atcgctcgca gggcggactg gggctgggac ttgcgttggt
    17101 caggcggctg gcagagctgc atggcggcag cgtgtccgcg agcagccccg gcccgaatgc
    17161 gggcagcagt ttcacactgc ggctgccgcg cgtgcgcggt acgcgcgtgg tcaaggatgc
    17221 ggctggccca gccggtgcgc ccgcgcgccg tgtggttccg ctgcgcatca tggtcgtcga
    17281 cgacaatgaa gacgccgccc acagcctggc catgctgctg ggcgcggaag gccaccaggt
    17341 gagcaccgcg gcgcgcgccg atgccgcgct ggcgctcgcc cgcgagcagg ccccccaggt
    17401 ttgcctgctc gacatcggcc tgcccggaat ggacggctat gaactggcgc ggcgcctgcg
    17461 cgcgctgtcc cagacgcgcg acgcgatgct gatcgcgctg accggctatg gacagccgca
    17521 ggaccgggct cgctcgttcg aagccggttt cgaccatcac ctcgtgaaac cggccgagat
    17581 ccggcgactg ctcgatctcg tctcccgccg cgccgcctga gctgcggcgt tgcgggcagg
    17641 cggaccaccc gcccatcgcc ttcgtccccc ttctcccttc cttcttcgat tccacgcggc
    17701 accgcgtggt cgcgtgcagt ccggaaagtc tcgatttggg gcacggctgt aaaaccttca
    17761 tggagaaatt acatccaatt tgcatgaagt gcgccgatgt ttccctgcgc acctcttctg
    17821 gcggcccgcg tgcgcatggc acaacgcttg ctgatcggtg aacgcaataa cacgcgtgcg
    17881 ccgcgtccgt gtcgcctgaa cgcggccggc caggccgcgc atcgcccgtt tcagggcgtc
    17941 gccgtagcag ccacatcgcg gaatcgttga aacgacggac gcgccgtgcg tcgttctcat
    18001 ttcttcaacc atcatgacca aggagcgata catggaaaac acgaactaca cgacctccca
    18061 gaccaccggc aacggcagcg cggcaaccag agatgcggcg aacaaacttt acagcgacgc
    18121 caagggcgcc gcgcagcgca ccggcgagac gctgcgcagc gaactgtcgg tgttgaaaca
    18181 ggacctggac gacctcatga gccgctcgtc gagcatgagc gacgaggaac tttcccgcgc
    18241 gcatgcccag ctgatggcca agttcagctc gctgcgctat gccgcgcgcg gattcgcgga
    18301 cgaagccacg cgccagtgga cgcgcggaat ggaaacgacc aaccagtacg tgaaggaaaa
    18361 gcccatgcag tcggtcggtc tggcggtcgg caccggtctc ctgctcggcc tgctgttctc
    18421 gcgccgctga ccgggagcgc ggcccgcccg ggccgccgcc tgactccacg aacaacacaa
    18481 ggattcgacg cgatgtttac cgccctgcac aagtccaagc agctctacct catcacgctc
    18541 gatcgcgtaa ccgattacat cgacctgctg aaggtcgaga tgaagatacg cgaataccag
    18601 ctggccttcc gcatggccgg cttcgccgtc gccctgctgt tcggcctgct tgcgacgatc
    18661 ttcctcggcc tggcgatcat catcagcttc tgggatacgg ccttcagggc gctcgcggcg
    18721 tggggtgtcg tcattctcta cggcggggtg gccggcgtga gcctctacta ctgcgtcaaa
    18781 catttccgct cgccgccgct gggcgggatg ctgcgcaatg aattgcaacg cgatatcaac
    18841 gtgatcaagg aaaacctatg agcaccgttc aatccctgga gcaggaaagg caggcgatcc
    18901 tcgaccgcat gcagatgcgg cgcgagactt accggcgcat gctggccgac ggcgaggacc
    18961 cgcgcatgct ggccgaaacg cacgaacagt accggcaagg ccacggcgac ggccgtgccg
    19021 gcgaatcggc ggccaaccgt gcggcgcacg ccgatccgcg cagcatgaag cgcttcgccg
    19081 cagccggtgt cggcgacgac tccaccgtct acacctatcg ccgcgtgaat gacagctttc
    19141 cgcgcacgcg ggtgatgcag gtggtggccg agcatccggt gctgtgtgca ctgggcgcgg
    19201 cggccgtgct ggccatcggc ccgcgccgca tcatgcgcac ggtcgcaacc agcagcgcag
    19261 ccatcggcgc ggtcgcccaa agccccaccg cgatggacat gatcgggcgc gtgctcacgc
    19321 tggccggtgc ggtcgcacag cggcgcaacg gctgaaatgc cagcggcgac atgtaacgca
    19381 atgttgctcc gagtggcatg gagtttgcag gacgtaagac aaggaagccc gaaacaagag
    19441 tgcccggttt cggcgaccga atgacagcca aatacttatc aatgaaagga gaagccatga
    19501 aaaaaatcat ttccgtactg agcgtgctgg tgatgtcgat cggcgtgtcc gcttgcaaca
    19561 cgatggccgg catgggccag gacatcgaac gcggcggcga gcgcatgcag ggtgccgccg
    19621 agcgccagca acaggagatg cagcgccgct gagcaccgct ccatgatttg gaaaaaccgg
    19681 ccatgcgccg gttttttttt gaactgcatc gcagatgaaa gaaaggagcg cacatgtctc
    19741 atatctacaa ggccggcgat ggcgctgctg gcgccgctgg cgccatgcgg atgacgagtt
    19801 ccccggcgtc gtcgagcgca tgccggaggg caagaccgga aactggatca tcggcggcag
    19861 gaaagtggtg gtgaggcagc gaaccgagct ggacgaggat cgcgggccgt ttcgcgtcgg
    19921 cgcgtgcgtg gaggtcgagt acgacgaccg cgaagtcgag gagctggaaa ccaagcagcc
    19981 cgagcgctgc cagggacgtt gaacagcatc agcggctcag gtcttcagcg gctgctcgac
    20041 cacgtcttcc acgtacagca gtcgcaccgc cacgatgaga ctcgcggtca gcggcgaggc
    20101 cagcaccacg ccgagcgggc cggtcagcgc cgccagcagg atggtggcga aaatgacgag
    20161 cgcgggcggc aacgacaccg tacgctgttc gatcagcggc gtgagcaggt agccttcgag
    20221 tgcctggatg ccgatgtaca gcaacaccac gtacaggccc agctccggat cgatgttgaa
    20281 ggcgatcagc accgggaccg cggcgccgag tatcggcccg acgtatggaa tgaattccag
    20341 cacgccggtg agcacgccga gcgtaaacgc atacggcacg ccgagcagcc acagcccggt
    20401 tccggccgcc acgccgacca gcgtcatcgt tgccaggcgc ccgaccagcc accagcgcag
    20461 ggcgaccccg atctcgtcca tcacctcgta cgcgcggggc cggcggcggc gcggcagcag
    20521 gtgcatgatg ccgcgctggt acaggcgcgg ctgcgcggcc agatagatgc cgatgaagat
    20581 caccaccagc gcgccgccga gcgccgccgc cgtcgatgaa aggatccagg tcgcgcgtcc
    20641 gagcgtgagc gtgtcgagcc ggatgtgcga cagctccacg accagcggct gcagcggccg
    20701 cgcccacctg tagtcaccca gccaggcacg caagcgcgcc atggcttgcg gcagtgtttc
    20761 aatcagcgag ccgacctgct cggccagagg gaacgcgaac agatacagca tgccgatcag
    20821 cccgccgagt atcgcgacga ctgacaggcc cagcgccgcc ccctcgccga gcgacgcatg
    20881 ttcggtgagc cagttggcgg gagcgcgcag cagcacggcg aacagcacgc cgccgaac
//
LOCUS       DATLGF010000527         4507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_110017, whole genome shotgun sequence.
ACCESSION   DATLGF010000527 DATLGF010000000
VERSION     DATLGF010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110017"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1634)
                     /locus_tag="VM406_16555"
     CDS             complement(<1..1634)
                     /locus_tag="VM406_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HVL77627.1"
                     /translation="MRRYAVSSPEPPANQGTRDDWATIKTLFPYLWQYKWRVMLALAC
                     LVAAKLANVGVPLVLKELIDSLTIAPGDPAALLVLPVGVLVAYGLLRLGTTVFTELRE
                     FVFAKVTQRAVRTVALQVFRHLHNLSLRFHLNRQTGGMTRDIERGTRGISSLVSYTLY
                     SILPTLVEITLVIGYLLLNYDIWFAAITFGALLTYIVFTVVVTEWRTNFRRTMNELDS
                     KANVRAIDSLINYETVKYFGNEEYEARRYDQSMQNWEAAAVKSQTSLSLLNSGQAMII
                     AIAVTLILWRATQGVIDGTMSLGDLVLVNAFMIQLYIPMNFLGVVYREIKQSLADMER
                     LFALLDQHKEVADAPGAKPLAVRGAEVRFSHVDFSYEPKRQILFDVDFTIPAGTTTAV
                     VGHSGSGKSTLSRLLFRFYDVNRGSITIDGQDLRDVTQASLRRAIGIVPQDTVLFNDT
                     IEYNIAYGRPEASREEIIAAAKAAYIHEFIESLPDGYATMVGERGLKLSGGEKQRVAI
                     ARTLLKNPAILVFDEATSALDSRAEQAIQAQLKEIAR"
     gene            1722..2129
                     /locus_tag="VM406_16560"
     CDS             1722..2129
                     /locus_tag="VM406_16560"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HVL77628.1"
                     /translation="MSSTQQTTLPEGKMPQLRVMPMPADANVHGDVFGGWIMAQVDIA
                     GSLPAVRRANGRVATIAVNSFLFKQPVFVGDLLSFYADIVKVGTTSITVNVEVYAERN
                     RLQVETVKVTEATLTYVATGEDRKPRPVPPLME"
     gene            complement(2349..4031)
                     /locus_tag="VM406_16565"
     CDS             complement(2349..4031)
                     /locus_tag="VM406_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HVL77629.1"
                     /translation="MEKFWLKSYPEGIPAEIDPSQYQSLVHLMEESFQKFANRNAYVC
                     MDKFMTYGELDLYSKQLAAWLQSRGLPKGARVAIMMPNVLQYPVALAAVLRAGYTVVN
                     VNPLYTPRELEHQMKDSGAEAIIILENFAHTLEKVISHTKIKHVVVASMGEMLGGVKG
                     MIVNLVVRRVKKMVPPFSLPNATRFKQALSEGARMSLKPVKLTHEDVAFLQYTGGTTG
                     VSKGATLLHRNVIANVLQNDAWAQPALAKEPKVDPLTIVCALPLYHIFALTACCLMGT
                     RWGAMNILIPNPRDIPGFVKELSKYKFNLLPAVNTLYNALLNNPDFAKLDFSMLKICN
                     GGGMAVQKAVAERWLAVTGCPIVEGYGLSETSPTATINPADATEFSGSIGLPIPSTEI
                     AILDDDGNKLPIGQNGEIAIRGPQVMAGYWNRPDETAKVMTADGFFKTGDIGTIDERG
                     FVRIVDRKKDMILVSGFNVYPNEVEGIVAEHPGVLEVACVGVPDAHSGEAVKLFVVRK
                     DPSLTADQLMEYCKQNLTGYKKPKYIEFREELPKTNVGKILRRELRDEKKAA"
     gene            complement(4227..>4507)
                     /locus_tag="VM406_16570"
     CDS             complement(4227..>4507)
                     /locus_tag="VM406_16570"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HVL77630.1"
                     /translation="YQELKQAWGGYSGYDRWFSEPLSNAHLSAIATYHDFVPAFRALL
                     AHEGDPARFYAAVKRLATLNKEDRHRQLAALAAPPTMAVHETGGPAPH"
BASE COUNT          841 a         1385 c         1412 g          869 t
ORIGIN      
        1 cgggcgattt ccttcaactg cgcctggatc gcctgctccg cacgggaatc aagagccgac
       61 gtggcctcat cgaagaccag gatcgccgga ttcttcagca aggtccgggc gatcgccacg
      121 cgctgctttt cgccgccgga caacttcagg ccgcgctcgc cgaccatggt cgcatagccg
      181 tcgggcaggc tttcgatgaa ttcatggatg taggccgcct ttgctgccgc aatgatctct
      241 tcccggctcg cttccggccg gccatatgca atgttgtact cgatcgtgtc gttgaacagc
      301 accgtgtcct ggggcacgat gccgattgcg cgccgcagcg aagcctgggt cacgtcgcgc
      361 aggtcctgac catcgatcgt gatgctgccg cgattgacgt catagaagcg aaacagcagg
      421 cgcgacagcg tcgacttccc tgagccgctg tggccgacca ccgcggtggt ggtcccggcg
      481 ggaatcgtga agtccacatc gaacaggatc tgtcgcttcg gttcatagct gaaatcgacg
      541 tgggagaaac gcacttcggc gccgcgcacg gccaagggct tcgctcccgg cgcgtccgct
      601 acttccttgt gctggtcgag cagcgcgaac aggcgctcca tgtcggccag gctctgcttg
      661 atttcacgat agaccacgcc gaggaaattc atcggaatgt agagctggat catgaacgcg
      721 ttgaccagaa cgagatcgcc gagactcatc gtgccgtcga tcactccctg ggtcgcgcgc
      781 cacaggatca aggtgacggc gatcgcaatg atcatcgcct gcccgctgtt aagcagcgac
      841 agcgaagtct gcgacttgac cgcggcggcc tcccagttct gcatgctctg gtcgtaacgc
      901 cgcgcctcgt actcttcgtt cccgaagtac ttgaccgtct cgtagttgat cagtgaatcg
      961 atcgcgcgca cattcgcttt ggaatcgagc tcgttcatcg tgcgccggaa gttcgtgcgc
     1021 cactcggtga ccacgacggt gaacacaatg taggtcagca aggcgccgaa ggtgatcgcg
     1081 gcgaaccaga tgtcgtagtt cagcagcagg tagccgatca ccagcgtgat ctcgaccagc
     1141 gtgggcagga tgctgtagag cgtgtatgac accagcgacg agatgccgcg cgtaccgcgc
     1201 tcgatgtcgc gcgtcatgcc gccggtctgg cggttcaggt gaaagcgcag cgaaagattg
     1261 tgcaggtggc gaaacacctg cagcgcaacc gtgcgcaccg cccgttgcgt aaccttggcg
     1321 aacacgaact cgcgcagttc cgtgaacacc gtcgtgccaa gccggagcaa gccgtaggcg
     1381 accaggacgc cgaccggcaa aacgagcaaa gccgcgggat cgcccggcgc gatcgtcagg
     1441 ctgtcgatca gctccttgag gaccagcggc acaccgacgt tggcaagctt ggccgcaacc
     1501 aggcaggcca aggcgagcat cacccgccac ttgtattgcc aaagataggg aaacagggtc
     1561 ttgatggtcg cccagtcgtc gcgcgtgcct tgattggcag gcggctccgg ggaagaaacg
     1621 gcgtaacggc gcatgaatgg caatcgcgat ggtttatgct tgccgattgt agccgagggg
     1681 cccgaaggcc accgtccacc accaccgcca ggagattgcc tatgagttcc acccagcaga
     1741 ccaccttgcc cgaagggaaa atgccacagt tgcgcgtgat gccgatgccg gccgatgcca
     1801 atgtgcatgg cgacgtgttc ggcggctgga tcatggccca ggtggacatt gccggctcgc
     1861 tgccggcagt gcggcgggca aatggccggg tggcgacgat tgcggtgaac tccttcctgt
     1921 tcaagcaacc ggtgttcgtc ggggatctgc tgtcgttcta cgccgacatc gtgaaggtgg
     1981 gcaccacctc gatcacggtc aacgtcgagg tgtatgccga gcgcaaccgg ctgcaggtgg
     2041 aaacggtgaa ggtgacggaa gccacgctca cctacgtcgc gaccggcgag gatcgcaagc
     2101 cgcgcccggt gccgccgcta atggagtgaa ataacggcct gggacgggag aggctgttcg
     2161 cttcagcgac cgcgcgatgc ctgtcgcccc gcgcaggcgg gggcccaatc cgaacgtgtg
     2221 cagctccgct gaagcctgcg ctgggtcccc gccttcgcgg ggacgacatg gcaagggcaa
     2281 tcgcgctagc gcacgcaatc gctttacagc gctgatgccc gaccggcgtt tcttcgacag
     2341 gaacagcttc aggcggcctt cttctcgtcg cgtaactcgc gccgcagaat cttgccgaca
     2401 ttcgtcttcg gcaactcttc acggaactcg atgtacttcg gcttcttgta gcccgtcagg
     2461 ttttgcttgc agtactccat cagctgatcg gccgtcagcg acggatcctt gcgcaccacg
     2521 aacagcttga ccgcctcgcc ggagtgcgca tcgggaacgc cgacgcaagc cacttcgagc
     2581 acgcccggat gctccgcgac gatgccttcc acctcgtttg gatacacgtt gaagccggac
     2641 accaggatca tgtccttctt gcggtcgacg atgcgcacga agccgcgctc gtcgatggtg
     2701 ccgatgtcac cggtcttgaa gaaaccgtcg gccgtcatga ccttggcggt ttcgtccggc
     2761 cggttccagt agcccgccat cacctgcggg ccgcgaatgg cgatttcgcc gttctgtccg
     2821 atcgggagct tgttgccgtc gtcgtccagg atcgcaatct cggtggacgg gatcggcagg
     2881 ccgatggagc cggagaactc ggtcgcgtcc gccgggttga tggtcgccgt cggagaggtc
     2941 tcggacaagc cgtagccttc aacgatcggg cagccggtca ctgccagcca gcgctcggcc
     3001 accgccttct gcaccgccat gccaccgccg ttgcagatct tcagcatgga gaaatcgagc
     3061 ttcgcgaagt cggggttgtt gagcagcgcg ttgtacagcg tattgactgc gggcagcaga
     3121 ttgaacttgt acttcgacag ctccttcacg aagccgggaa tgtcgcgcgg attcggaatc
     3181 aggatgttca tcgcgcccca gcgggtaccc atcaggcagc aggcggtcag cgcgaagatg
     3241 tgatacagcg gcagcgcaca aacgatggtc agcggatcga ccttcggctc cttcgccagc
     3301 gccggttggg cccaggcatc gttctgcagc acgttggcga tcacattgcg gtggagcagc
     3361 gtggcaccct tcgatacacc ggtggtgccg ccggtgtact ggaggaaggc gacatcctcg
     3421 tgagtgagct tgaccggctt gagcgacatg cgcgcgccct cggacaaggc ctgcttgaag
     3481 cgggtggcgt tgggcagcga gaacggcggc accattttct tcacgcggcg caccaccagg
     3541 ttgacgatca tgcccttcac gccgcccagc atctcgccca tgctggctac cacgacgtgc
     3601 ttgatcttcg tgtgggagat caccttctcg agcgtatgcg cgaagttctc cagaatgatg
     3661 atcgcttcgg cgcccgagtc cttcatctgg tgctcgagtt cgcgcggcgt gtacagcgga
     3721 ttgacgttga ccaccgtgta cccggcgcgc agcaccgcgg cgagcgcgac cggatactgc
     3781 agcacgttgg gcatcatgat cgccacacgc gcccctttcg gaaggccacg gctctgcagc
     3841 caagcggcca gctgtttcga atacaggtcc agttcgccgt acgtcatgaa cttgtccatg
     3901 catacgtagg cgttacggtt cgcgaacttc tggaaggact cctccatcag gtgtaccagc
     3961 gactggtact gcgaaggatc gatctctgcg gggatgccct cggggtagct cttcagccaa
     4021 aatttttcca tgtctgcctc tagcctctga ttgttgtgat gtgggatagc aaaaataatc
     4081 gaacgatggt tctatttttt ggatagatgc tatcggggct agaggttcag tgcaagtctt
     4141 ttggtcacat gcggtggcgc tttgcgagcg tcgcatcgag gcaggttgcg gcgcacaaaa
     4201 aaagcggccc gcaggccgct cggacatcaa tgcggcgcgg ggccgcccgt ttcatgcacc
     4261 gccatcgtcg gcggcgccgc gagcgccgcg agctggcgat gacgatcttc cttgttcagg
     4321 gtggccaggc gcttgaccgc cgcatagaag cgcgcgggat ccccctcatg cgccagcagc
     4381 gcccggaacg cgggcacaaa gtcgtgatag gtggcgattg cgctcagatg cgcgttcgac
     4441 agcggttcgg aaaaccagcg gtcatagccg ctgtagccgc cccatgcctg ctttagttcc
     4501 tgatagt
//
LOCUS       DATLGF010000528         2066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_110288, whole genome shotgun sequence.
ACCESSION   DATLGF010000528 DATLGF010000000
VERSION     DATLGF010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110288"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VM406_16575"
     CDS             <1..920
                     /locus_tag="VM406_16575"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HVL77631.1"
                     /translation="YIDGGKPRNLIVVREGDNFKASEVDAALERRVEMHSGEIRSSLF
                     AATDAAQIPDSVAMQIVDMFATNINFASDLRRGDRFNVVYETFWQNGEFVRAGRVLAG
                     EFINAGKRYQSVWFEEPGTSGGYYTFDGKALKKAFLKYPLEFSRISSGFSLRVHPISG
                     QWRRHQGVDFAAPTGTPIRAAADGVIDFIGVQGGYGNFIVIKHWNNITTAYAHMSRFA
                     SGLRKGAKVSQGEVIGYVGTTGWSTGPHLHYEFRVNNVPRDPMSVEIPNAQPLTGAEL
                     QRFRTVAAEMTHRFALLNQQEEAIKVAAR"
     gene            1019..>2066
                     /locus_tag="VM406_16580"
     CDS             1019..>2066
                     /locus_tag="VM406_16580"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HVL77632.1"
                     /translation="MSGTSMDGVDAALASFSESDGRLVVRTVASASRPFPSSLQTELH
                     ALQAPGDNEVHREALAANQLARLYAETALAAVKEAGIEPAAIRALGAHGQTIRHRPEL
                     CYTRQIGNPALLAELTGIDVVADLRSRDIAAGGQGAPLVPAFHYEVFGDPAATRVVVN
                     IGGIANISVLAPGQSVIGFDTGPGNVLLDYWCMRHLGQAYDADGAWAASGRVLPDLLQ
                     IMLAEPYLDLPPPKSTGRDLFHAGWLETRLAAAAAGAPGDVQATLTEYTATTIARAIL
                     RYARGTSEVYVCGGGACNATLLAALTRNLEQRDAPIRVDTTAALGVEPQLVEALAFAW
                     LAHRHVARLPGNLPS"
BASE COUNT          351 a          736 c          645 g          334 t
ORIGIN      
        1 cctatatcga cggcggcaag ccgcgcaacc tgatcgtcgt gcgcgaaggc gacaacttca
       61 aggcaagtga agtagacgcg gcgctcgagc gccgcgtcga gatgcactcc ggcgagatcc
      121 gctcctcgct gttcgccgcg accgacgccg cgcagattcc ggacagcgtc gcaatgcaga
      181 tcgtcgacat gttcgccacc aacatcaact ttgcgtccga ccttcgccgc ggcgaccgct
      241 tcaatgtcgt ctatgaaacc ttctggcaga acggtgagtt cgtgcgcgcc ggacgcgtgc
      301 tggctggcga attcatcaac gccggcaagc gctaccagtc ggtctggttc gaggaaccgg
      361 gcaccagcgg cggctactac acgttcgacg gcaaggcgct gaagaaggcc ttcctcaagt
      421 atccgttgga gttttcccgg atctcgtccg gcttctcgct gcgcgtgcac ccgatctcgg
      481 gccagtggcg ccgccaccag ggcgtggact ttgccgcgcc caccggcacc ccgatccggg
      541 cggccgcgga tggcgtgatc gacttcatcg gcgtgcaggg cggttacggc aatttcatcg
      601 tgatcaagca ctggaacaac atcaccaccg cctacgcgca catgagccgg ttcgccagcg
      661 gactgcgcaa gggcgccaag gtgagccagg gcgaggtcat cggctatgtc ggcaccaccg
      721 gctggtcgac cggcccccac ctgcattacg agttccgtgt gaacaacgtg ccgcgcgatc
      781 cgatgtcagt tgagattcca aacgcccagc cgctcaccgg cgccgaactg cagcgcttcc
      841 gcacagtcgc agccgagatg acccaccgct tcgcactgct caaccagcag gaagaggcga
      901 tcaaggtcgc ggcgcgttga cctgtttcgg gaccggtccc aagccggtcc cgtccctccc
      961 agcaatcctc tcccccggct cttcgatgga cgcccacgct cctctcttcc tcggcctgat
     1021 gtcgggcaca agcatggacg gcgtcgacgc cgccctcgct tcgttttccg aaagcgacgg
     1081 acgcctggtc gtccgcaccg tggccagcgc ctcgcgcccc ttcccctcgt cgctgcagac
     1141 cgaactccat gcgctgcagg ctcccggcga caacgaagtg cacagggaag cacttgccgc
     1201 caatcagctg gcgcgcctgt acgcggagac cgcgctcgcg gcggtcaagg aagccggcat
     1261 cgagcctgct gcgatacggg cgctcggcgc ccacggccag acaatccgcc atcgcccgga
     1321 actgtgctac acccggcaga tcggtaaccc ggcgctgctc gccgaactga ccggcatcga
     1381 tgtggtcgcc gacttgcgca gccgcgacat tgcggccgga ggccagggcg caccgctggt
     1441 gccggcgttt cattacgaag tgttcggcga tcccgctgcc acgcgggtcg tggtcaacat
     1501 cggcggcatc gccaacatca gcgtgctggc gcccgggcag tccgtgatcg gattcgatac
     1561 cgggccgggc aatgtcctgc tcgattactg gtgcatgcgc cacctcggcc aggcctatga
     1621 tgcggacggc gcctgggcgg caagcgggcg ggtgctgccc gacctgctgc aaatcatgct
     1681 ggcggagccc tatctggacc tgcctccgcc caagagcacg gggcgcgatc tgttccacgc
     1741 cggttggctc gagacccggc ttgccgcggc agcggctggc gcaccggggg atgtccaggc
     1801 aaccctgacc gaatacacgg ccacgacgat cgcccgcgcg atcctgcgtt atgcgcgggg
     1861 caccagcgaa gtctatgtat gcggaggcgg cgcatgcaac gccacgctgc ttgccgcgct
     1921 cacccgcaat ctcgagcaac gcgacgcccc catacgcgtc gacaccacgg ccgcactggg
     1981 tgtggagccg caactggtcg aagcgctcgc cttcgcctgg ctcgcccacc gtcatgtggc
     2041 acgcctgccg ggcaacctgc cgagcg
//
LOCUS       DATLGF010000529         4938 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_110586, whole genome shotgun sequence.
ACCESSION   DATLGF010000529 DATLGF010000000
VERSION     DATLGF010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110586"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..401)
                     /locus_tag="VM406_16585"
     CDS             complement(63..401)
                     /locus_tag="VM406_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HVL77633.1"
                     /translation="MLKKLALAAVAAWMMVGAATAQVDVNKADVVALDGIKGIGPSTS
                     KAILEERGKGGEYKSWSDFESRVKGVGGKKAAALSQAGLTVNGQARPADTKTGDAGAK
                     AATKASPERK"
     gene            complement(505..1434)
                     /gene="rfaE1"
                     /locus_tag="VM406_16590"
     CDS             complement(505..1434)
                     /gene="rfaE1"
                     /locus_tag="VM406_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HVL77634.1"
                     /translation="MNPVLPAFSNARVLVAGDVMLDRYWFGDVSRISPEAPVPVVRVE
                     RCEERLGGAANVARNIVSLGATAGLLGVVGQDEAGDTLLSLIDALRVQGLLYRDPAIS
                     TVVKLRVIGRQQQLLRIDFEEPPSATVLREKLSQFTSLLPRYDVVVLSDYAKGSLVDV
                     ADMISAARKLGKCTLVDPKGEDFSRYRGASILTPNKGELREVVGSWKSEDELANKAQR
                     LRAELELDALLLTRSEEGMSLYTQGAIRHFPALAREVYDVSGAGDTVIATLAVMLGAG
                     MPLPDAVSLANRAGGIVVGKLGTATVSRDELLP"
     gene            complement(1431..2804)
                     /locus_tag="VM406_16595"
     CDS             complement(1431..2804)
                     /locus_tag="VM406_16595"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011660457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HVL77635.1"
                     /translation="MKISVIGTGYVGLVTGVCLAELGHDVMCVDVDQDKIDLLREGCV
                     PIHESGLAELLRRNRRARRLHFSSDIDTGVRHGEVLFIAVGTPAAPDGSANLCSVFDA
                     AQSIAAAMDTFRLIVIKSTVPVGTAERVGELVRKGLEERALDCSFCVLSNPEFLKEGV
                     AVRDFMAPDRIVIGCPEGEAGERARAMMREVYAGLDPAGSRCLWMAPRAAEFTKYAAN
                     AMLATRVSFINEMANLADAMGVDIEAVRCGIGADRRIGAAFLQAGCGYGGSCLPKDVQ
                     ALRHTARQAGLATPLLDAVDAVNARQKRILFDKIVRRFGSDLQGRCFAMWGLTFKPNT
                     DDMREAPSRVLIRLLLDSGAAISMYDPVAMAEAQRVLYQDHDGFYDLQQRLRFGAGPL
                     QVLDGADALVIATDWQEFRQVDPAQISMQLKSPVVFDGRNLYAPAAAAAAGLEYYAIG
                     RSAARPS"
     gene            complement(2838..4013)
                     /gene="lapB"
                     /locus_tag="VM406_16600"
     CDS             complement(2838..4013)
                     /gene="lapB"
                     /locus_tag="VM406_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HVL77636.1"
                     /translation="MEFETWWLLAIPVFFGLGWIAARVDIKQLVSESRTLPRGYFKGL
                     NLLLNDQPDKAIDAFIEIVTLDPETIELHFALGNLFRRRGEIERAIRVHQNLLARADL
                     PTEYKEQSQFELGQDYLKAGLLDRAEETFNLLVDTRYGAQARRALLEIYQREKEWMRA
                     IEAARALQESGAGGRQKEIAQFYCELAQDALVHTDTTRALELLDQALSADRHNVRATI
                     LGGDVHRACGDMEAALHAWQRVEHQSVPHVALVAQRLMDGYRTVGRAQEGVNLLKNYL
                     AEAPSIDLLEVVFKAVLEQDGVEAANQLVSNELRRSPTLLGLDKLLEARLMNAPPEVV
                     PELSFVKNLVHGYTHKLARYQCSHCGFKARQFYWQCPGCSQWETYPPRRTEELNVIS"
     gene            complement(4047..4355)
                     /locus_tag="VM406_16605"
     CDS             complement(4047..4355)
                     /locus_tag="VM406_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HVL77637.1"
                     /translation="MKYVFRIVAVLLFLVFFGFALKNTQDVALEFFWGYELRGPLVLL
                     LLGFFVAGAALGILAMLPTVFRHRRDLSQHKQRLAVYEQQQMRRQSTTPPSADSIVNP
                     "
     gene            complement(4518..4823)
                     /locus_tag="VM406_16610"
     CDS             complement(4518..4823)
                     /locus_tag="VM406_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HVL77638.1"
                     /translation="MTKSELIARLAERYPQLVAKDADFAVKTILDAMTDALSRGQRIE
                     IRGFGSFALNSRPPRIGRNPKSGDKVMVPEKRVPHFKPGKDLRERVDAMVGQPIKED"
BASE COUNT          923 a         1624 c         1511 g          880 t
ORIGIN      
        1 gtaatcggag tggccgatcg ggtcggcgag gcaaatgccc cgcatacgcg gggcattttc
       61 gtctacttgc gctccggcga cgctttcgtc gccgccttgg cgcccgcgtc ccccgtcttc
      121 gtatccgccg gccgcgcctg accgttgacc gtcaggccgg cctgggacag cgcagccgcc
      181 ttcttgccgc cgacgccctt gacgcggctc tcgaagtccg accagctctt gtactcgccc
      241 cccttgcccc gctcttccag aatggccttg gaggtggaag ggccgatgcc cttgatgcca
      301 tccagggcca cgacatcggc cttgttcacg tcgacctgcg ccgtcgcggc accgaccatc
      361 atccaggccg caacagcggc gagggctagc ttcttcagca tgaatttctc ctgttcgtca
      421 aaaaatggac aatttcccga tgattgcctg aatgacaatc atcggggcga aattaccatt
      481 ttgataatta tttgacaaca ggaatcaagg cagcagctca tcccggctga ccgtcgcggt
      541 accaagcttg ccgacgacga ttccacctgc ccggttggcc agggatacgg cgtccggcag
      601 cggcatgccg gcgccaagca tgaccgcgag tgtggcaatc acggtgtcgc ccgctccgga
      661 gacgtcgtag acttcgcgcg ccagggccgg gaagtgacgg atcgctccct gcgtgtagag
      721 cgacatgccc tcctcagacc gggtcagaag cagcgcatcc agttccagct cggcgcgcag
      781 gcgctgggcc ttgttcgcaa gttcgtcttc gctcttccag gaaccgacca cctcgcgcag
      841 ctcccccttg ttcggcgtga ggatgctggc gccgcggtac cgcgaaaaat cctccccctt
      901 cggatccacc agcgtgcact tgcccagctt gcgcgccgcg gaaatcatgt cggccacgtc
      961 gaccaggctg cctttcgcat agtcggacag gacaaccacg tcgtagcgcg gcagcaatga
     1021 cgtgaactgg ctgagcttct cgcgcaggac cgtggccgac ggcggttcct cgaaatcaat
     1081 gcgcagcaac tgctgctgac ggccgatcac gcgcagcttc accacggtcg aaattgccgg
     1141 atcgcggtac agcaagcctt gcacgcgcag tgcatcgatc aagctgagca gcgtgtcgcc
     1201 cgcctcgtcc tggccgacca cacccagcag tccggccgtg gccccgagcg agacgatatt
     1261 tcgcgcgaca ttggcggcgc cgcccagccg ctcctcgcag cgttcgaccc gcaccaccgg
     1321 taccggcgcc tctggagaaa tgcggctgac gtcgccgaac cagtagcgat caagcatgac
     1381 gtctccggcc accagcacgc gcgcgttcga gaaggcgggc aataccggat tcatgatggc
     1441 ctcgctgcgg accggccgat cgcgtaatac tccaacccgg cagccgcggc agcagcgggc
     1501 gcatagaggt tgcggccgtc gaagacgacc ggcgacttca gctgcatgga aatctgtgcc
     1561 gggtcgacct ggcggaactc ctgccagtcg gtcgcaatga caagagcgtc cgcgccgtca
     1621 agcacttgca gcggaccggc gccgaagcgc aggcgctgct gcagatcgta gaagccgtca
     1681 tggtcctggt acagcacccg ctgcgcttcg gccatcgcaa ccggatcgta catcgagata
     1741 gcggcaccac tgtcgagcag caggcggatc agcacgcgtg acggcgcctc gcgcatgtcg
     1801 tcggtattcg gcttgaacgt gagcccccac atcgcaaagc accgcccttg cagatcgctg
     1861 ccgaagcgac gcacgatctt gtcgaagagg atgcgcttct gcctcgcatt gaccgcgtcc
     1921 accgcgtcga gaagcggcgt ggcaagtccc gcctggcggg cggtatggcg caatgcctgg
     1981 acgtcctttg gcagacagga gccaccgtag ccgcatccgg cctgaaggaa ggcggcccct
     2041 atcctgcgat cggcgccgat gccgcaccgc accgcctcga tgtccacccc catcgcgtcg
     2101 gccaggttcg ccatctcgtt gatgaacgac acccgcgtcg caagcatcgc gttggccgcg
     2161 tacttggtga actcggcggc gcggggcgcc atccagaggc agcgggagcc cgccgggtca
     2221 aggcccgcgt acacttcgcg catcatcgcg cgcgcgcgct ccccggcctc gccctccgga
     2281 cagccgatga cgatgcggtc aggcgccatg aaatcgcgca ccgccacccc ctccttcagg
     2341 aactcgggat tggacaggac gcagaatgaa cagtccagag cgcgttcttc gagacctttt
     2401 cgcaccagct cgccgacccg ttcagcggtg ccgaccggga ccgtggactt gatcacgatc
     2461 aggcggaacg tgtccatcgc ggcggcaatg gattgcgccg cgtcgaacac gctgcacagg
     2521 ttcgcggagc cgtccggcgc ggccggggtg ccgacggcga tgaagagcac ctcgccgtgc
     2581 cttacgccgg tatcgatgtc ggaggaaaaa tgcagacggc gtgcgcgccg gttgcgcctg
     2641 aggagctcgg caagcccgct ttcatggatc ggaacgcagc cttcgcgcag caggtcgatc
     2701 ttgtcctgat cgacgtcgac gcacatgaca tcgtggccca gctccgccag gcacactcct
     2761 gtgacaagtc cgacgtagcc agttccgatg accgatattt tcatgcgcgc acgatccgtg
     2821 atcgccgcgc ccgcacatca gctgatgacg ttcagctcct cggtccggcg cggcggatag
     2881 gtttcccatt ggctgcagcc ggggcattgc cagtagaact gccgtgcctt gaagccgcag
     2941 tggctgcact gatagcgagc cagcttgtgg gtatagccgt gcacaaggtt cttgacgaac
     3001 gacagttcgg gaacgacctc gggcggcgcg ttcatcagac gcgcctcgag cagcttgtcc
     3061 agtccaagca gcgtcggcga acgccgcaac tcgttactga ccagctgatt ggccgcctcc
     3121 acgccatcct gctcaagaac cgccttgaac acgacttcca acaaatcaat ggagggagct
     3181 tcggcaagat agttctttag caagttgacg ccttcttgag cgcggccaac agttcggtag
     3241 ccatccatga gccgctgggc gacgagggcc acgtgcggca cgctctgatg ctcgacgcgc
     3301 tgccacgcgt gcagcgcggc ctccatgtcg ccgcaggcgc gatgcacatc gccgcccagg
     3361 atcgtggcgc gcacgttgtg ccggtccgcg ctcagcgcct ggtcgagcag ttcgagcgcg
     3421 cgcgtggtat cggtatgcac gagcgcatcc tgggcaagct cgcaatagaa ctgcgcgatt
     3481 tccttctggc ggccccctgc acccgactcc tgcagcgcgc gcgccgcctc gatcgcacgc
     3541 atccactcct tctcgcgttg atagatttca agcagcgcac gccgcgcctg ggcgccgtag
     3601 cgggtatcca ccaacaggtt gaaggtttcc tctgcgcggt cgagcagccc ggccttcagg
     3661 taatcctggc caagttcgaa ttgcgactgc tccttgtact cagtcggcag gtccgcgcgc
     3721 gcaagcaggt tctggtggac gcgaatcgcg cgctcgatct cgccgcggcg ccggaacagg
     3781 ttgcccagcg caaaatgcag ttcgatagtt tccggatcga gcgtgacgat ttcgatgaag
     3841 gcatctatcg ccttgtccgg ctggtcattg agaaggaggt ttagtccctt gaagtaacca
     3901 cggggcagtg tccgggactc ggaaaccagc tgcttgatgt cgacccgggc ggcaatccag
     3961 ccgagtccga agaaaacagg gatggcaagc agccaccaag tttcaaattc catgagaagg
     4021 tattgatgtg ttgtcggcgc ggcagctcaa gggttgacga tactgtccgc cgaaggcggg
     4081 gttgtgctct ggcggcgcat ctgctgctgt tcgtacacgg ccagccgctg cttgtgctgc
     4141 gagagatcgc gccggtgccg gaacacggtc ggaagcatgg ccagaatgcc gagcgccgcg
     4201 ccggccacaa aaaaaccgag cagcagcagg accagcgggc cgcgcaactc gtatccccag
     4261 aaaaattcca gcgccacgtc ctgggtgttc ttcagcgcaa atccgaaaaa gacgaggaag
     4321 agaaggactg caacgattcg gaagacatat ttcatgacgc aaccttcttg atgaggagct
     4381 ggaacgttgg tgcgcggcgg gggctggcgc tggcgcgaaa cggcattgta cgtgaaaaaa
     4441 aacggggact tgaccggcgg cacaggcgta aaaaaacggc atccgaagat gccgtttctt
     4501 gcccatctga aaataaccta gtcttccttg atcggctggc ccaccatcgc gtccacccgt
     4561 tcgcgcaagt ccttgcccgg cttgaagtgg ggcacccgct tctcgggaac catcaccttg
     4621 tcgccggact tcggattacg gccgatccga ggcggccggc tgttcagcgc aaagctgccg
     4681 aacccgcgga tctcgatgcg ctgtccgcgg gacagcgcat cggtcatcgc atcgaggatg
     4741 gtcttgaccg cgaaatccgc atcctttgcc accagctgcg gataacgctc ggccagacga
     4801 gcaatcagct ccgacttagt cattgacggc tcgttgctta ttgcttgctg tccagcttgg
     4861 ccttgagcag tgcgcccagg ctggtggtgc ccgaagcggc agccgagtcg gccgacatct
     4921 tctgcatcgc ttcctgcg
//
LOCUS       DATLGF010000530         4027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_110753, whole genome shotgun sequence.
ACCESSION   DATLGF010000530 DATLGF010000000
VERSION     DATLGF010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110753"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1282)
                     /locus_tag="VM406_16615"
     CDS             complement(<1..1282)
                     /locus_tag="VM406_16615"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HVL77639.1"
                     /translation="MSPQNRPEAGAAGLVLGVVGCGVMGRGIAQIAALGGAEVRMCDS
                     QAGAADAALTTLQQTFAKLAEKGKLDAADARDAAARVQPCGDLAELAGCHVVVEAIVE
                     NLDAKKTLFANLEQVVDAETILATNTSSLSVTAIAAGLRHPARVAGFHFFNPVPLMKI
                     VEVIAGFHTAPQVTDYLLALGRRWGHTAVRASDTPGFIVNHAGRGYGTEGLRLLAEGV
                     ASIATLDEILRDTAGFRLGPCELLDLTGLDVSHPVMESVYRQYYEEPRFRPQVLTRQM
                     LVAGALGRKTGHGFYRYDADGRKIAAPEPGQAPTAMPETVWLSGERPDLAERVGPMIA
                     AAGVSVESGARPSERALCIVTPLGEDATACCMRENLDARRTVAVESLFDTSRRRVVMT
                     TPATSAETLAMARGLFGATGIPVSVIRDSTGLVTQ"
     gene            1657..2484
                     /locus_tag="VM406_16620"
     CDS             1657..2484
                     /locus_tag="VM406_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HVL77640.1"
                     /translation="MAREQDTQELDDEEAQAVPAKPSNAKEDRHFVTALARGLEILSC
                     FRSGEKLLGNQELADRAKLPKSTVSRLTYTLTKLGYLQYDEDVGKYRLGTASLALGSA
                     MLSKLDIRQYARPYMQELADFTHATVSLGMRDRLSMIYVENCRSQSALTLRLDVGARI
                     PIAQTAMGRAYLAVCPVSERNDILERVRELDELKWPSIAQGVERAVEEYRTLGVCTSF
                     GDWQPDVNAIAIAFKPAHGSSILSVNCGGPSFKLSPEFLLQEARPRLIALADQLRQI"
     gene            2817..3992
                     /locus_tag="VM406_16625"
     CDS             2817..3992
                     /locus_tag="VM406_16625"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HVL77641.1"
                     /translation="MSEVVIVAAGRTAVGKFGGTLAKTPAADLGAHVIRQLLARTGIA
                     PDMVSEVIMGQVLTAGVGQNPARQAVIKAGLPDMVPGMTINKVCGSGLKATHLAAQAI
                     LCGDAEVVIAGGQENMSAAPHALNGSRDGFRMGDAKLVDTMIVDGLWDVYNQYHMGIT
                     AENVAKKYGVSREEQDAFAAASQNKAEAAQKEGRFKDEIIPFEIPSKKGAIVFDTDEF
                     IKHGVTAESLATLRPAFDKSGSVTAGNASGINDGAAAVIMMSARKADQLGLKPMARIK
                     AYSSAGVDPTIMGMGPVPASQLCLKKAGWSPQDLDLMEINEAFAAQAIGVNKEMGWDT
                     SKINVNGGAIAIGHPIGASGCRILVTLLHEMIRRDAKRGLASLCIGGGMGVALAVER"
BASE COUNT          719 a         1364 c         1279 g          665 t
ORIGIN      
        1 gctgggtgac caatccagtg ctgtcacgga tcacagagac cggaatgccg gtcgcgccga
       61 acagcccgcg cgccatcgcc agcgtctcgg cgctggttgc cggcgtcgtc atcaccaccc
      121 ggcgccggct cgtatcgaac aggctttcga ctgcgaccgt gcggcgcgca tcaaggtttt
      181 cgcgcatgca gcaggcggtc gcgtcctcgc cgagcggcgt cacgatgcac agcgcccgct
      241 cgctcggacg cgcaccggat tccacgctta cgccggccgc ggcgatcatc ggccccacgc
      301 gctcggccag gtccgggcgt tccccgctca gccagacggt ttccggcatc gcagtcggcg
      361 cttgcccggg ttcgggagcg gcgatcttcc tgccgtcggc gtcgtagcga tagaagccgt
      421 gcccggtctt gcgcccgagc gcgccggcca ccagcatctg gcgcgtcagc acctgcggcc
      481 ggaagcgcgg ctcctcgtag tactgccggt acaccgattc catcaccgga tgcgaaacat
      541 ccagtccggt caggtcgagc agttcgcacg ggccgaggcg gaaaccggcc gtgtcgcgca
      601 ggatctcgtc gagcgtcgcg atcgatgcca ccccctcggc cagcaggcgc aggccctcgg
      661 tgccgtagcc gcgccccgcg tggttgacga tgaagccggg cgtgtcgctg gcgcgcaccg
      721 cggtgtggcc ccagcggcga ccgagcgcga gcaggtaatc cgtcacctgc ggcgcggtat
      781 gaaagccggc aatcacctcg acgatcttca tcagcggaac cgggttgaag aaatgaaagc
      841 cggccacgcg cgcgggatgg cgcagcccgg cggcaatggc ggtgaccgac agcgaggagg
      901 tgttggtggc gagaatcgtc tccgcatcca ccacctgttc caggttcgcg aacaaggtct
      961 tcttcgcatc gaggttttcg acgatggcct cgaccaccac gtgacaaccg gccagttcgg
     1021 ccaggtcccc gcagggctgg acgcgcgccg ccgcgtcgcg cgcatcggcc gcatccagct
     1081 tgcccttttc cgcaagcttc gcgaacgtct gctgcaaggt cgtcagcgct gcgtcggccg
     1141 cgccggcctg gctgtcgcac atcctgacct ctgcgccacc cagcgccgcg atctgcgcaa
     1201 tgccgcgccc catcacgccg caaccgacca cgcccagcac caggccggcg gcgcccgctt
     1261 ccgggcggtt ttgcggactc attcctttct cctttgataa ttctgcacag cagaataaaa
     1321 tttcatttat cggaactcta agtatttgcc tgcgctctgt caaggcatcc aggccgggcc
     1381 catgcggacg ctcagaaaac tggccatggg accggacgtc gcggaattcc ggcatggcgt
     1441 gacgggaaac cactgtgcgg aatgcaaggc tcacaaatgc tttgcctttc gcgcctcgct
     1501 atgtcagtat cgaccgcagt gcagaacccg gatcaggtta gcgacggctt tgcacgccgc
     1561 ccttgattag ggataatgcg cggcgcaggg cagccaagcg atcgcatgcc cattccatca
     1621 tgcaggaccc gcgcctgcac actaagaaga accagcatgg cccgagagca agatacccag
     1681 gaattggacg atgaagaggc gcaggcggtg cccgccaagc cgtcgaacgc gaaggaggac
     1741 cgccacttcg tcaccgcgct cgcacgcggg ctcgagatcc tgtcctgctt tcgctccggc
     1801 gagaagctgc tcggcaacca ggagctggcc gaccgggcga agctgccaaa gtcgaccgtg
     1861 tcgcgcctga cctatacgct gaccaagctc ggctatctgc agtacgacga ggatgtcggc
     1921 aagtaccggc tcggcaccgc cagcctggcg ctcggcagcg cgatgctgtc caagctcgac
     1981 atccgccagt acgcgcgccc ctacatgcag gaactggcgg atttcaccca cgccaccgtt
     2041 tcgctcggca tgcgcgaccg cctgtcgatg atttacgtcg agaactgccg cagccagtcc
     2101 gcgctgacct tgcgcctgga cgtcggcgcc cgcattccga tcgcccagac cgcgatgggc
     2161 cgcgcctacc tggccgtgtg cccggtcagc gagcgcaacg acatcctcga gcgggtgcgc
     2221 gaactcgacg agctgaagtg gccgtcgatt gcgcaggggg tggaacgggc ggtggaggaa
     2281 taccgcacgc tcggcgtgtg cacctcgttc ggcgactggc agcccgacgt gaatgcgatt
     2341 gcgattgcct tcaagccggc ccacggcagc tccatccttt cggtgaactg cggcgggccc
     2401 tcgttcaagc tttctcccga gttccttctc caggaggcgc gtccgcgcct gattgcactg
     2461 gccgaccagc tgcgccagat atagggtctg ccggcaccca cgaaccgggt gttggcaggg
     2521 cctggttctt tctccgccgg cgcggcgctg acgggcaccc tgccgggtgc cggttttcat
     2581 tcgctttcat gatcgttttt gctatagtcg gctcatgctc gccgacccgt cagcggaacg
     2641 ttcgcccgca atattcgccg acctttcccg gtcctccgac ggttgcgctg gatcaagcgc
     2701 tgcactgtcc ggcccgtcta agatgcgcgc tttcccagtc gttgtccccg gccgacgcgc
     2761 acagatcacg catccctccc acacctcttc agcccatcca cccacggaga atcgaaatga
     2821 gtgaagtcgt catcgtcgcc gccggacgca cggcagtcgg aaaattcgga ggcaccctgg
     2881 ccaagacgcc ggccgcggat ctcggtgccc atgtaatcag gcagctgctc gccaggaccg
     2941 gtatcgcacc tgacatggtc agtgaagtga tcatggggca ggtcctgacc gcgggagtgg
     3001 ggcagaaccc ggcgcgccag gcagtgatca aggccggtct gcccgacatg gtgccgggta
     3061 tgaccatcaa caaggtgtgc ggctccgggc tgaaggccac ccatctggcc gcccaggcaa
     3121 tcctgtgcgg cgacgcagag gtcgttatcg ccggcgggca ggaaaacatg agcgcggccc
     3181 cgcacgcgct caacggctcg cgcgacggct tccggatggg ggacgcgaaa ctggtcgaca
     3241 cgatgatcgt ggacggcctg tgggacgtct acaaccagta ccacatgggc atcactgccg
     3301 agaacgtcgc gaagaaatac ggcgtctcgc gcgaggagca ggacgcgttc gccgcggcct
     3361 cgcagaacaa ggccgaggcc gcgcagaagg aggggcgttt caaggacgag atcatcccgt
     3421 tcgaaattcc atccaagaag ggcgcgatcg tgttcgacac cgacgaattc atcaagcacg
     3481 gcgtgaccgc cgaatcgctc gcaacgctgc gtccggcctt cgacaagagc ggcagcgtca
     3541 ccgcagggaa cgcgtccggc atcaacgacg gcgccgccgc ggtcatcatg atgtcggccc
     3601 gcaaggccga ccaactcggc ttgaagccga tggcgcgcat caaggcgtat tcctcggccg
     3661 gcgtggaccc gaccatcatg ggcatgggtc cggtgccggc atcccagctg tgcctgaaga
     3721 aggccggctg gtcgccccag gatctggatc tgatggagat caacgaagcc ttcgccgccc
     3781 aggcgatcgg cgtgaacaag gagatgggct gggacacctc gaagatcaac gtgaacggcg
     3841 gtgcgatcgc gatcggccac ccgatcggcg cgtcgggctg ccgcattctc gtcaccttgc
     3901 tgcatgaaat gatccgccgc gatgccaagc gcggactggc cagcctttgc atcggcggag
     3961 gcatgggcgt tgcactggca gtcgagcgct gactcgcgtc ggcgcactac ttgatgacac
     4021 ctagaac
//
LOCUS       DATLGF010000531         5994 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_126408, whole genome shotgun sequence.
ACCESSION   DATLGF010000531 DATLGF010000000
VERSION     DATLGF010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126408"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VM406_16630"
     CDS             complement(<1..655)
                     /locus_tag="VM406_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL77642.1"
                     /translation="MASLSVLRSLGLKAALAVLVAVTAGQAVAQAAKAPTKVRYEEVV
                     RSILYVPAYVALSQGYFKDAGLDVQMKTSQGTDKGMAALLSGSADIVLIGPEATVYVQ
                     NTESPVKPRIFSGLTATDGFILVSRSGEPFDWNKLKGKTIMAWRPGSNPDVYLETALR
                     KHGLDPKKDVTIMNNLGPAARAGAWMAGQADYAIFLEPEAGTLEREGKAYSVASIGAE
                     "
     gene            complement(1192..3630)
                     /locus_tag="VM406_16635"
     CDS             complement(1192..3630)
                     /locus_tag="VM406_16635"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HVL77643.1"
                     /translation="MASERRPESPWTVSYDHYDPDTERRREVVLALGNGVYVTRSTPV
                     DARPGPTHYPGTYRAGCYERIPGRVADTEDPTDSLANLPNWTRLDFRIDGGPWFSLDA
                     VEILDYAHWLDLKAGIATRSVLFLDAAGRRTRLCEERLVSMADPALCALRVALTPQDW
                     SGRIELRSTIDAEVRNTNVQRYIDYEQRHLRDPVWHRLSGNEGMVLSGTLHSGVDIAM
                     AFRTVIDRDDALADATAQDGGIEHRAELAVQPGHGVAIEKHVAICTSLDPEADEAGRR
                     AAAALVRARGFAPMHADHEQAWNRIWQRMGIEAEDPELARFLYLHAFHVMQTVSPLSA
                     PLDVGIPARGWHGEGYRGHIFWDELFVLPFLNFRFPELAREALMYRHRRLDAARDAAR
                     KAGWRGAMYPWRSARDGGEVTPRRQKNLLSGAWMEDHTWRQHHIGAAIAFNTWQYYLV
                     TGDREFLLQCGADMMLEIARFWASIASPGGEDNSYDITGVVGPDEYHNAYPGRSEAGL
                     DNNAYTNLMAAWTLVHALRMLDHLESAERTALLERLGIDEEELRHWDHVSRNLRVRFL
                     DDGVIAQFEGFERLKPFSKDVLPPELEDKRLDWALHKIGQSADDYQLSKQADTLTLFY
                     LIPYHEAAELLQRLGCEFDRDRLMRTVEYYLDRTTHRSSLSATVYAGALAGEDLDRSW
                     EFYRQALHTDLEGRKGESIAEGVHLAAMGGTLDVLQRRYLGVRPTEFGLCIEPNLPRQ
                     LGVVRLGIWFRGAHLELESRGDRVCVRASAGNAAAVPVMRDGVQVMLEPGGEMEFEAV
                     SPALDGESGRAAQG"
     gene            complement(3620..4588)
                     /locus_tag="VM406_16640"
     CDS             complement(3620..4588)
                     /locus_tag="VM406_16640"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HVL77644.1"
                     /translation="MNEERRGHVLALGSINADFQMRVARRPEISETLIGKDFLRLGGG
                     KSANVALIARRLGLPSLLLAHVGDDDLAEQALAPLRDAGVDISGVSSIAGEATGVAFI
                     LVPPDGKKGIVMCANANDAWRQADLDAVRTAMRGAPEDSVLVTNGEIPAVAVEEGMRS
                     ARSRGITTVLDPSPADKVGDALLRLADVVTPNSGEAETLTGIACDTPQAARRAAQQLM
                     QRGASAAAVKLADGGCLFADADGWVHIGTTPVRVVDTTGAGDAFAGAFASLVARGASW
                     REAARFAVAAAHLAVGGYGSQPSYPEFEPIRELAAALPIMEGAHGE"
     gene            complement(4601..5611)
                     /locus_tag="VM406_16645"
     CDS             complement(4601..5611)
                     /locus_tag="VM406_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL77645.1"
                     /translation="MKPLAIQARACLRGLLLLTCVLQLGACASLTQPGPADRQAIAGK
                     TFVVTGASSGIGRAVAHELAASGANIVLAARRAEVLDEVAGEVRRVGGTPLVVVTDVS
                     RAQEMERLAQAAIGHFGRIDVWINNAGVGALGRFEDVPARDHARVIDVNVNGVIYGSH
                     AALRQFIAQGRGTLVNISSVQGQIPTPYQSSYAASKAAVLGLGRALNQELRLARRDRI
                     RVVTVLPWAVDTPFFEHAANYTGRAARSIAYDDPTMVARAIVWVSLHPRDEFPVGWKA
                     GGAYAGHNLLPALTERVAADVEHATQIESGPPAPPTSGNLHQPVRGGATVEGGLRSHS
                     AR"
     gene            complement(5704..>5994)
                     /locus_tag="VM406_16650"
     CDS             complement(5704..>5994)
                     /locus_tag="VM406_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVL77646.1"
                     /translation="LIPSSSHYDVTHMTTAVAMVRQGLGITVLPRLAVPELNVTGMVC
                     KPLKDASARRTIGLLHRRDRGLSPACQVFSEKVRAAILQVESSLPPLRAAPR"
BASE COUNT          978 a         2067 c         1922 g         1027 t
ORIGIN      
        1 cttcggcgcc gatcgaagcc acggagtaag ccttgccttc gcgctccagc gtgccggcct
       61 cgggctcgag gaagatcgcg tagtcggcct gtccggccat ccacgcgccg gctcgcgcgg
      121 ccggtcccag gttgttcatg atcgtgacgt ccttcttcgg atcgaggcca tgcttgcgca
      181 acgccgtctc cagatagaca tcgggattcg aacccggccg ccaggccatg atggtcttgc
      241 ccttgagctt gttccagtcg aacggctcgc cgctgcgtga aacgaggatg aaaccatcgg
      301 tcgcggtaag gccggagaag atgcgcggct tgaccggcga ctcggtgttc tgcacgtaga
      361 ccgtcgcttc cggcccgatc aggacaatgt ccgcactgcc ggacagcagt gcggccatgc
      421 ccttgtcggt gccctgcgag gtcttcatct ggacatccag tcccgcatcc ttgaaatagc
      481 cctgagagag cgcgacatag gccggcacgt agagaatcga tcgcaccacc tcctcgtagc
      541 gcaccttggt cggcgccttg gcagcctgcg ccaccgcttg ccccgccgtg accgccacca
      601 atacggccag agccgccttc agacctagac ttctgagaac actgagcgat gccatcgcaa
      661 ctccttcagg ttatgttgac gggaaaaggc tgtgcacgtg agtccgggat ggtgaatatc
      721 agatattggt atactttata caggctctgg aacttgtcaa ccaacttcgt cgcacagaag
      781 ccaagggcgc atccttgcgg cgtttccaag gggtctccct taggaacctg cccgcttccg
      841 gtgcggcgcc gcaccatcct tcacacgccc cgcaccacaa gcaaacagat tttctgtata
      901 cagtattctt ctgctccgct cgtgcacggc cgcatgcgaa aacgcaagtc ccatgcctga
      961 aagcatcgca tcgggcgatc ggtgactatt gatgaggatc aattgcggag ggtgtgggga
     1021 gatcgctggt ccgggccctc gggatgatgt gtgccacttc ccctccgcgt cctcaccatg
     1081 cgagccccag cgatgtcgtc cccgcgcagg cggggaccca gctccgcagt atgcagagac
     1141 gacgacgaag ccagtaccgt gggcccgtcg ccgcgtgcgc ggctgtgcgc atcacccctg
     1201 cgcggcgcgc ccgctctccc cgtccagcgc cggcgatacc gcttcgaact ccatctcgcc
     1261 gccgggctcc agcatgacct gcaccccgtc gcgcatcacc ggcaccgcag cagcgttgcc
     1321 ggcactggcg cgcacgcaga cccgatcgcc gcggctttcg agttccagat gcgcgccgcg
     1381 aaaccagatc ccgagccgta ctacgccaag ctgcctcggc agattcggtt cgatgcacaa
     1441 gccaaattcg gtcggacgca cgccgaggta acggcgctgc agcacgtcca gcgtgccgcc
     1501 catggccgca aggtgcacgc cttcggcaat gctctccccc ttccgcccct ctaggtcggt
     1561 gtgcagggcc tgcctgtaga actcccacga gcgatccaga tcctcgcccg ccagcgcgcc
     1621 ggcatacacc gttgccgaca ggctggagcg atgggtcgtg cgatccagat agtactcgac
     1681 cgtgcgcatc agccggtcgc gatcaaactc gcaacccagc cgctggagca gctcggccgc
     1741 ctcgtggtag gggatcaggt agaacagggt cagcgtgtcg gcctgcttgc tgagctggta
     1801 gtcgtccgcg ctttggccga tcttgtgcag tgcccagtcc agccgcttgt cttccagctc
     1861 tggcggcagc acgtccttgc tgaacggttt caggcgctcg aagccctcga actgggcaat
     1921 gacgccgtca tccagaaagc gcacgcgcag attgcggctc acatgatccc agtgccgcaa
     1981 ctcctcctcg tctatgccga gccgctcaag caaggcggtg cgctcggcgg attcgaggtg
     2041 gtcgagcatg cgcagcgcat gcaccagtgt ccaggccgcc atcaggttcg tgtaggcgtt
     2101 gttgtcgagg ccggcctcgc tgcgtccggg ataggcgttg tgatattcgt cgggcccgac
     2161 cacgcccgtg atgtcgtagc tgttatcttc gccgccagga gacgcgatgc tggcccagaa
     2221 ccgggcgatc tccaacatca tgtcggcgcc gcattgcagg agaaattcac gatcgccggt
     2281 gacaaggtag tactgccagg tattgaaggc gatcgccgcg ccgatatgat gctggcgcca
     2341 cgtgtggtcc tccatccatg cgcccgaaag caggttcttc tgccgccgcg gcgtgacttc
     2401 gccgccgtcg cgcgcgctgc gccacggata catcgcgccg cgccagccgg ccttgcgggc
     2461 cgcgtcgcgc gccgcatcga ggcgacggtg gcggtacatc aatgcttcgc gagccagctc
     2521 ggggaaccgg aagttcagga atgggagcac gaacagctcg tcccagaaga tatgcccgcg
     2581 atagccttcg ccgtgccagc cgcgcgccgg aatgccgaca tcaagcggtg cactcagcgg
     2641 cgagacggtc tgcatcacgt ggaatgcgtg caggtacagg aaccgggcca gctccgggtc
     2701 ctcggcttcg attcccatgc gctgccagat gcggttccat gcctgttcgt ggtcggcgtg
     2761 catcggtgcg aagccgcggg cgcggaccag cgcggctgcc gcgcgccggc cggcctcgtc
     2821 ggcctcaggg tcgagcgagg tgcagatcgc gacatgcttt tcgatcgcca cgccgtgtcc
     2881 cggctgcacc gcgagctccg cgcggtgctc gatcccgcca tcctgtgctg tcgcatcagc
     2941 cagtgcatca tcgcggtcga tcactgtcct gaacgccatc gcgatgtcca caccgctgtg
     3001 cagcgtgccg ctcaggacca tgccctcgtt tccggacagt cggtgccaaa ccgggtcgcg
     3061 caggtggcgc tgttcgtaat cgatatatcg ctggacgttg gtgttgcgca cctcggcgtc
     3121 gatggtggaa cgcagctcga tgcggccgga ccagtcctgc ggtgtgagcg cgacacgcaa
     3181 ggcgcacagc gccggatcgg ccatgctcac cagtcgttcc tcgcagaggc gggtgcgccg
     3241 acccgccgca tcgaggaaga ggacgctgcg cgtcgcgatg ccggctttca ggtcgagcca
     3301 gtgcgcgtag tcgaggatct cgaccgcgtc gagcgaaaac cagggtccgc cgtcgatgcg
     3361 gaagtcgagc cgcgtccagt tcggcaggtt cgccaggctg tccgtcgggt cctcggtatc
     3421 cgccaccctg cccggtatgc gctcgtagca acccgcgcgg taggtgcctg gatagtgagt
     3481 cggtccgggc ctggcatcga ccggcgtgct gcgtgtcacg tacacgccat ttcccagcgc
     3541 gagcaccact tcgcgtcggc gctcggtgtc gggatcgtag tggtcgtagc tgactgtcca
     3601 tggggactcg gggcgtcgct cactcgccat gcgcgccctc cataatcggc agagccgccg
     3661 ccagttcccg gatcggctcg aattccggat aggacggctg cgagccgtag ccaccgacgg
     3721 ccagatgggc ggcggcgacg gcgaaccgtg ccgcttcgcg ccagcttgcg ccgcgcgcga
     3781 cgaggctggc aaaggcgccg gcaaacgcgt caccggcgcc ggtcgtgtcg accacgcgca
     3841 caggcgtcgt tccgatatgc acccagccgt ccgcatcagc gaacaggcaa ccgccgtccg
     3901 ccagcttcac cgctgcggct gatgcgccgc gctgcatcag ctgctgcgcg gcgcgtcgcg
     3961 ccgcctgcgg cgtatcgcac gcgattccgg tcagggtttc agcttcgccg ctgttgggcg
     4021 tgaccacgtc ggccaggcgc agcagcgcat cgccgacctt gtcggccggc gagggatcga
     4081 gcacggtcgt gatcccgcgg ctgcgcgcgc ttcgcatgcc ctcctcgacc gccactgcag
     4141 gaatctcgcc gttcgtcacc agcaccgaat cctcgggcgc gccgcgcatc gccgtgcgca
     4201 ccgcatcgag atccgcctgc cgccatgcgt cgttggcatt ggcgcacatc acgatcccct
     4261 tcttgccgtc gggcggcacc aggatgaagg ccacgccggt cgcctcgcct gcgatgctgc
     4321 tgaccccgga tatgtcgacg cccgcatcgc gcagcggggc cagcgcctgc tcggccagat
     4381 cgtcgtcgcc gacgtgtgcg agcagcagtg aaggcaggcc gagacggcgc gcaatcaagg
     4441 ccacgttggc cgacttgccg ccgcccaggc gcaggaaatc cttgccgatc agggtctcgc
     4501 tgatctccgg ccggcgcgca acgcgcatct ggaaatcggc gttgatgctg cccagcgcca
     4561 gcacatgtcc tctccgttct tcgttcatca cactccttta ttaccgggct gaatggctgc
     4621 gcagcccgcc ttcgaccgtc gcgccgccgc gcaccggctg gtgcaggttt cctgaagtgg
     4681 gcggggccgg cggcccgctc tcgatctgcg tcgcatgctc gacgtcggcg gcgacgcgct
     4741 cggtcagcgc cggcagcagg ttgtggccgg cgtaggcgcc gccggccttc cagccgaccg
     4801 ggaactcgtc gcgcggatgc agcgacaccc agacgatggc gcgcgcaacc atggttggat
     4861 cgtcataggc gatcgaacgc gccgctcgtc cggtgtagtt ggccgcatgc tcgaaaaacg
     4921 gcgtgtccac cgcccagggc agcacggtca cgacccgtat ccgatcccgg cgcgccagcc
     4981 gcagttcctg attcagcgca cgcccgaggc ccaggacggc cgccttcgaa gccgcatagc
     5041 tgctctggta cggcgtcgga atctgcccct gcacggaact gatgttgacc agcgtgccgc
     5101 ggccttgggc aatgaactgg cgcagcgcgg catggctgcc gtagatcacg ccgttgacgt
     5161 tgacgtcgat cacccgcgca tgatcgcgag ccggcacgtc ctcgaagcgt ccgagcgcgc
     5221 cgacgccggc gttgttgatc cagacgtcga tgcggccgaa gtggccgatg gctgcctgcg
     5281 cgaggcgctc catttcctgg gcccggctga catcggtgac caccaccaac ggtgttccgc
     5341 ccacccgcct cacctccccg gcaacctcgt ccagcacctc ggcacgtctt gccgccagca
     5401 caatgttggc gccggaagcc gccaattcgt gcgcgacggc gcggccgatt ccgctggacg
     5461 cgcccgtcac gacgaaagtc ttgccggcaa tggcctgacg gtcggccggg ccgggctggg
     5521 tgagggaggc gcaggcccca agctgcagga cgcaggtgag cagcagcagc ccgcgcagac
     5581 aggcccgcgc ctgtatcgcc aaaggcttca tatcgcctcc cgcagggaaa aatctccgct
     5641 aaagcaaacc gcattcccat tgacaaatgg acagctggcg aacaggaaag ggccggtggg
     5701 cgttcagcgg ggggccgcac gcagtggcgg caggctgctc tccacctgca ggatcgcggc
     5761 gcggaccttt tcagagaaca cctggcatgc cggcgacagc ccccggtcgc gccggtgcag
     5821 caagccgatg gtgcggcgtg cgctcgcatc cttcagcggc ttgcacacca tgcccgtcac
     5881 attgagttcc ggcacagcca ggcgtggcag cacggtgatg ccgaggcctt ggcgcaccat
     5941 ggccactgcg gtggtcatgt gcgtcacgtc gtagtgcgag gagctgggta tgag
//
LOCUS       DATLGF010000532         3334 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Noviherbaspirillum sp. isolate SMAG_U13607
            k141_189695, whole genome shotgun sequence.
ACCESSION   DATLGF010000532 DATLGF010000000
VERSION     DATLGF010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939489
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Noviherbaspirillum sp. (soil metagenome)
  ORGANISM  Noviherbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Noviherbaspirillum.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:14:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,301
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Noviherbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189695"
                     /isolate="SMAG_U13607"
                     /isolation_source="soil"
                     /db_xref="taxon:1926288"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VM406_16655"
     CDS             complement(<1..602)
                     /locus_tag="VM406_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HVL77647.1"
                     /translation="MAAVTHTRRMHGVAPEPERPMRVVARDLQAMEVLAEHNHPWGQL
                     TYAVEGVVRVTAGNSTWIVPPQRAIWVPPLLSHEVTTLERARLRALYVLAEVAPFQDG
                     GCKVLEVSPLLREAIVALADAEPASLRERRLAELLLDELARLPTLPIALPLPADKRLK
                     ALCEKLIADPASTETLDDWAEQVGASARTLARLFERELGM"
     gene            711..1958
                     /locus_tag="VM406_16660"
     CDS             711..1958
                     /locus_tag="VM406_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL77648.1"
                     /translation="MSASTASRPPSTFASDSKVITLVGVAHAVSHFFHLILAPLFPWL
                     KDDFGVSYAELGMLMTVFFVVSGVGQALAGFVVDRVGARAVLFAGLGLLGVSALVLSQ
                     AQNYWMLLFGAAIAGLGNSVFHPADFTLLNKRVSTTRLGHAFSVHGITGNLGWVAAPI
                     FLIPLTELFGWRAALAGAALIPAAVLLLLYLNRGLLEGGEARPRHAAGPAAADAHALA
                     FLRLPGVWMCMLFFFIIAMALGGIQNFAVPALRDMYGVTLAAATAGYTAFMLASAVGM
                     VLGGFIAARATRHERTIAIAFAASGMFALLIASGLLHGLAAVALMAAVGFGSGVAGPS
                     RDLMIRDAAPANATGRVYGVVYSGLDVGLAGAPLMFGALMDAGQPAWLFVCVGILNCA
                     AVLAAVGVGTRTVRVPAATQAAG"
     gene            1997..2491
                     /gene="rraA"
                     /locus_tag="VM406_16665"
     CDS             1997..2491
                     /gene="rraA"
                     /locus_tag="VM406_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HVL77649.1"
                     /translation="MSFATCDLCDNHEDKLGDGTLAVLPPVFQKFGKRTAFSGRAATL
                     KVFEDNVLVRAALETPGEGRVLVVDGGGSLRCALVGGNLGALAEKNGWAGIIVNGCIR
                     DSDEINACDIGVRALATHPQRSVRKGVGERELRVLIAGTAVYPGDWIYADADGVLISR
                     QALA"
     gene            2913..>3334
                     /locus_tag="VM406_16670"
     CDS             2913..>3334
                     /locus_tag="VM406_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HVL77650.1"
                     /translation="MTMATREEQIKALEKDWAENPRWKGIKRNYTAEDVVRLRGSKQI
                     EYTLAKEGAEKLWAKVNGGAKKGYVNAFGAITAGQAMQQAKAGLEAVYLSGWQVAADG
                     NTSETMYPDQSLYAYDSVPTMVRRINNTFKRADEIQWSR"
BASE COUNT          534 a         1076 c         1074 g          650 t
ORIGIN      
        1 ctcattccca gttcgcgctc gaaaagacgc gccagcgtgc gcgccgaggc cccgacctgc
       61 tcggcccagt cgtccagcgt ctcggtcgag gcaggatcgg cgatcagctt ttcgcacagc
      121 gctttcaagc gcttgtcggc aggaaggggc agggcgatcg gcaaggtcgg caggcgtgcg
      181 agctcgtcga gcagcagttc ggccagccgc cgttcgcgca ggctcgcagg ctccgcgtcg
      241 gccagcgcga cgatggcctc gcgcagcagc ggcgagactt ccagcacctt gcagcccccg
      301 tcctggaacg gagcaacttc cgccagcacg tacaaggcgc gcagccgcgc ccgctccagc
      361 gtcgtcactt catggctgag cagcggcggc acccagatcg cgcgctgcgg cgggacgatc
      421 caggtgctgt tgccggcggt cacgcgcacg acgccttcga ccgcatacgt cagctgaccc
      481 cagggatggt tgtgttcggc cagcacctcc atggcctgca ggtcgcgcgc gacgacccgc
      541 atcggccgct cgggctccgg cgcgacaccg tgcatgcgac gggtatgggt aacggcagcc
      601 attgctcgtc tccgggtttg gcggtttttc gataaagttt gtcgctctat cttaaagcag
      661 acggcgggcc tttgcgtaca cttgcgccat ccaatcactg ttcgtccgcc atgtccgcat
      721 ccaccgcctc ccgaccgccg tccacgtttg ccagcgattc caaggtgatc accttggtcg
      781 gtgtcgccca cgccgtctcc cacttcttcc acctgatact cgcgccgctg ttcccttggc
      841 tgaaggacga tttcggcgtc tcctatgccg agctcggcat gctgatgacg gtgttcttcg
      901 tggtatcggg tgtcggccag gcgcttgccg gcttcgtggt cgaccgcgtc ggcgcgcgtg
      961 ccgttctgtt tgccggactg ggtctgctcg gcgtgtcggc gcttgtgctg tcgcaggcgc
     1021 agaactactg gatgctgctc ttcggtgccg cgatcgccgg tctcggcaac agtgtcttcc
     1081 accctgccga tttcacgctg ctcaacaagc gcgtgtccac aacgcgcctt ggacacgctt
     1141 tctcggtgca tggcattacc ggcaatctgg gatgggtcgc cgcgccgatt ttcctgatcc
     1201 cgctcaccga gctgttcggc tggcgcgcgg cgctcgccgg ggcggcgttg atcccggcag
     1261 ccgtactgct cttgctttac ctgaatcgcg gtctgctgga aggcggcgag gccaggccgc
     1321 gccatgccgc cggaccggcc gccgcggatg cgcacgcact cgccttcctg cggcttccgg
     1381 gggtgtggat gtgcatgctg ttcttcttca ttatcgcgat ggcgttgggc ggcatccaga
     1441 atttcgcagt gcccgcactg cgcgacatgt acggcgtgac gctcgcggcc gcaaccgccg
     1501 gctataccgc cttcatgctc gcgtcggcgg tgggcatggt gctcggaggc ttcatcgccg
     1561 cacgggcaac acgccatgaa cgcacgattg cgatcgcgtt tgctgcctcg ggcatgttcg
     1621 cgctgctgat cgccagcggg ttgctgcacg gtcttgccgc ggtcgcgctg atggccgcag
     1681 tcggcttcgg ttccggcgtc gccgggcctt cgcgcgacct gatgatccgc gacgccgcgc
     1741 cggcgaatgc gaccggccgc gtgtacggcg tcgtgtactc gggtctggac gtgggcctgg
     1801 cgggcgcacc gttgatgttc ggcgcactga tggatgccgg ccagcctgcc tggctgttcg
     1861 tctgcgtggg cattctcaat tgcgcagcgg tgctggccgc ggtcggcgtg ggcacccgta
     1921 ccgtgcgcgt gccggccgcg acgcaggccg cgggctgagg cacaatctgc catttgttca
     1981 acgaatggag actccgatgt cctttgccac ctgcgacctg tgcgacaacc atgaagacaa
     2041 gctgggcgac ggcacgcttg ccgtgctgcc tccggtgttc cagaagttcg gcaagcgcac
     2101 cgcgttctcc gggcgcgccg cgacgctgaa ggtcttcgag gacaacgtgc tggtgcgcgc
     2161 tgcactggag acgccgggcg agggccgcgt gctggtggtc gacggcggcg gcagcctgcg
     2221 ctgcgcgctg gtcggcggca atctcggcgc gctcgcggag aaaaacggtt gggccgggat
     2281 catcgtcaac ggctgcatcc gcgattccga cgaaatcaac gcctgcgaca tcggtgtgcg
     2341 ggcactcgcc acgcatccgc agcgaagcgt gcgcaagggc gtaggcgaac gcgagctgcg
     2401 cgtgctgatc gccggtacgg ccgtatatcc cggcgactgg atctatgccg atgccgacgg
     2461 cgttctcata tcgcgtcagg cgcttgcgtg agttccccag gaggccacgc cgcctccatg
     2521 aacacggcgg cgcttcgacc gccgccgctc gcgcgccatg cgcacgttga gggctttccg
     2581 gatctgaatg gagtggagaa gcccgcgttg cgtcccgctt gcttccctcc acgtgcagga
     2641 ctgcttgcgc tttcacaggc gcgtcggatg ctatttcgtg atgcaaaaga tgcgtttcat
     2701 aatgtgaaag cgcctacggc agtgcgtcgg ggtccgattt cattttaagg aaaaacgttc
     2761 cgtatcatga aaatacggat ataagtattt gattttaagc gataaaaaaa tacttatatg
     2821 tcttatataa gaccttgttg ccccgcaaaa aactccctat catttctcca tcggtttccg
     2881 cttttttctt ttcacctcgc atcgacagga gaatgaccat ggcaactcgc gaagagcaaa
     2941 tcaaggccct tgagaaggac tgggctgaaa acccgcgctg gaagggcatc aagcgcaact
     3001 acaccgcgga agacgtggtg cgcctgcgcg gctccaagca gatcgagtac acgctggcca
     3061 aggaaggcgc cgagaagctg tgggccaagg tcaacggcgg cgcgaagaag ggctatgtga
     3121 acgcgttcgg cgcgatcacc gcaggccagg cgatgcagca ggccaaggcc ggtctggaag
     3181 ccgtctacct gtccggctgg caggttgcag cggacggcaa cacctccgaa acgatgtacc
     3241 cggaccagtc gctgtatgcg tacgactcgg ttccgaccat ggtccgccgc atcaacaaca
     3301 ccttcaagcg cgccgacgag atccagtggt cgcg
//
